LOCUS       DASUVS010000001         5290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_218432, whole genome shotgun sequence.
ACCESSION   DASUVS010000001 DASUVS010000000
VERSION     DASUVS010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218432"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VF182_00005"
     CDS             <1..950
                     /locus_tag="VF182_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6435482.1"
                     /translation="IHSHVDCLSFPLARFASVPTVSTMHGRLDVKELLPVYRHYKDQP
                     VISISHDQRRPLPELNWVGTIYHGLPRNLLKFRSEPGDYLAFLGRLSPEKRPDLAIEI
                     ARRSGIPLKIAAKIDRADREYFETVIRPLLSAPGIEFIGEINEAQKQDFLGRALALLF
                     PVDWPEPFGLVMIEALACGTPVIARPCGSVPEVLRQGISGFIESSIDDLVQAVHKVGD
                     IPRRTCRDEFERRFTAEVMADNYEQVYYQLADMRRMTQGAAVRTKRAIHDAALAREAT
                     RHWHRTPEAKSLDTRRKSEAPVLTLFSNCDERDPTNLDS"
     gene            complement(1271..1909)
                     /locus_tag="VF182_00010"
     CDS             complement(1271..1909)
                     /locus_tag="VF182_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX6435483.1"
                     /translation="MTRTLGLQLALLLIVSGCRSERFDTIRNIGSVGDTIVCFGDSLT
                     EGVGAKPGDAYPTVLSRLLGMRVLNAGRRGDTTAQALERIHASVLSKNPRLVILLLGG
                     NDFLRQVPVRETQMNLKEIVRQIQGHGAMVAIAGIKLGFFTDEYGPLFEETAAELGAV
                     YIPQVMKGIFNDAKLRSDQIHPNSAGYRLMAERIAERVKPLLDEADRLRGRL"
     gene            complement(2638..2886)
                     /locus_tag="VF182_00015"
     CDS             complement(2638..2886)
                     /locus_tag="VF182_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012141599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435484.1"
                     /translation="MVDDGFKTVLWVLAAVFFVSACAHIKEAGRTIGHTTRDVTREIG
                     HGTRDVAKEIGQGTRDAANTVGDGVKKVVKPSTPPAEY"
     gene            3139..4779
                     /locus_tag="VF182_00020"
     CDS             3139..4779
                     /locus_tag="VF182_00020"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX6435485.1"
                     /translation="MSAINLRSAHAQPSNGNIVRFLEQATFGPTPLLIAHLEQVGIEA
                     YLNAQYTAPMADYPELEFWPQMRPAGCTGDCQRDNYTYYQLQRHFFTNALYGADQLRQ
                     RIAFALSQILVTSQVDVPLPSWMRTYQQLLYKSAFGNFRELLYQITVNPTMGRFLDML
                     NNRCQTRTPPDVNICRNGLNSQPNENYAREILQLFSIGTFMLNQDGTRQLDANGDPIP
                     TYDQETVEEFARVFTGWILAPAIAGPSSAGGTVPNYRDPMRVRLDSQGREDYHDKGAK
                     QLLAGSRLPGGQSQSAELGAAIDNIAFHPNVAPFIGKQLIQHLVTSNPSPAYVARISA
                     VFASNANSPTQLFEVARAILLDPEARGDSVDAAAQPNYGKLREPVQFITNLLRAFGAT
                     SDGVLNTLNIGGSSIGSGDMGQDVFNAPSVFSFYPPTVRVPGENALGPQFALFSSLTS
                     LRRANFAQRIIFSNIPAAANRPTGTSIDLSSWDSLANDPDRLIEALNHLLLHGVMTAE
                     MHDAVKVAVESIPASNSRLRVRTAIYLIATSSQYQVQR"
     gene            4793..>5290
                     /locus_tag="VF182_00025"
     CDS             4793..>5290
                     /locus_tag="VF182_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435486.1"
                     /translation="MAMTRRKFLSRSLGALGAAMLGIERFGLLNALAQSADYRALVCV
                     FLNGGNDSGNMIIPYDDYPAYVAARGSSGLAIEQSSLLRTGSVPGIGAEFGFHPSLTA
                     LHDLWAEGKLAVACNVGTLVEPTTRDAYRNRTVRVPLNLFSHSDQINQWQTSVSDSAS
                     PSGWGG"
BASE COUNT         1213 a         1479 c         1372 g         1226 t
ORIGIN      
        1 tcattcattc gcatgttgac tgcctcagtt tccctctggc gcgatttgcc tcggtgccga
       61 ccgtatcgac catgcatggc cgtcttgatg tcaaggagct cttgccggtc tatcgacact
      121 ataaagatca gccggttatc tcgatcagcc acgatcagcg ccgtcccctc cctgagttga
      181 attgggtcgg caccatctat catggcttgc cgcgcaattt gttaaagttc cgctccgaac
      241 caggcgacta tttggctttt ttggggaggc tgtctccgga gaagcggccg gacctggcca
      301 tcgagattgc ccgtcgctca ggaataccac ttaagatcgc agcgaagatc gatcgagccg
      361 atcgcgaata cttcgagact gttatcaggc cgttgctatc ggcccccggc atagaattca
      421 tcggagaaat aaacgaggca cagaagcagg atttccttgg aagagcgctc gctttgctct
      481 tcccagtgga ttggcccgag cctttcgggc tggtgatgat cgaggcacta gcttgcggta
      541 ctcccgtcat tgccaggcca tgtggctccg taccggaagt tctccgtcag ggaatcagcg
      601 ggtttatcga atcctctatc gatgatctgg ttcaggcagt acacaaagtc ggtgatatcc
      661 cgcgccgcac atgccgcgac gaattcgaaa ggcgctttac cgccgaagtc atggccgaca
      721 actacgaaca ggtttactac caactggccg acatgcgccg catgacgcaa ggagccgccg
      781 tacgaacgaa acgtgcaatc cacgatgcgg cgcttgcccg agaagccacc cgacattggc
      841 accgaacgcc ggaagctaag agtttggaca cacgccggaa gagcgaagcg ccggttctga
      901 cgctattttc aaattgtgac gagagggatc cgacgaatct cgatagttag gttaggtcgc
      961 tgtaacccca acgtggaggg atgaacatgt cgaattcgga agtcactgct aaaacgagtg
     1021 aattactcga ttctttgaag ccctatggaa catatcctcg gaatccgggc aatgagggct
     1081 ccgcatcttc cagttcggaa ttcactgtgg gtgaactcta cgggtgcgtt tgtcatcggc
     1141 cgttcgatga acttgcacgg tcgcggcttg gatcgattcc cgccagtggc ggcctacaac
     1201 tcacagagta gtcgccgggg ttgtgcggga ttctttgatc cagttacttg catctaggga
     1261 gacattgcag ctacaaccgg cctcttaaac ggtccgcttc atccaacagc ggtttaaccc
     1321 tttcggcaat acgctcggcc atcaaacggt aaccggcgct gttggggtga atctgatcgc
     1381 ttctgagttt tgcatcattg aaaatacctt tcatcacctg cgggatatag actgcgccga
     1441 gttccgccgc tgtctcctcg aagagggggc catactcatc agtaaagaaa ccgagtttga
     1501 tgccggcgat tgccaccatc gcgccgtgac cttgtatttg ccggacgatt tcttttaagt
     1561 tcatttgagt ctccctaacg gggacttgac gtaaaaaatc atttccaccg agaagcaaaa
     1621 tcaccaggcg gggattcttg cttaaaaccg aagcatggat tcgttctagc gcttgcgcgg
     1681 tggtatcgcc gcggcgaccg gcattcagca cccgcattcc caacaggcgc gacaatacag
     1741 ttggataagc gtcccccggc ttggcgccaa ccccttccgt gaggctgtca ccgaagcaga
     1801 caatggtatc gccaacggag ccgatgttgc ggatggtatc aaatcgctca ctgcggcatc
     1861 cggaaacaat caggagtagg gcaagctgta atccgagagt acgagtcatc tagaatcgtt
     1921 aatcgggaga cgcagttcgg cccatccgaa cgtatacgct tcgccatgca ggtgccggac
     1981 gatcgagcgc cctaaacgat cggcataaat ctccaatgca gcttagaaaa tggatggctc
     2041 gggtcgcgca gtttgcctcc tagacatcag ctgatccttc gtcttcgcga ttctttttgc
     2101 ggattgtatt tttactgctg gaagtcccaa ccttttggct agccgggcgc atgaagagca
     2161 tacaaccata ttgagcagaa caacgcccgc ctcttccgtg attacgcgat agagtttttg
     2221 ctttgtaatg tgattgcagc gacggcaagt aaactccatt aattatctcc ttgcaatgcg
     2281 cgctaaacgc gcttgatgat cgctaattga attctcagtg tgtgaagagt aaaaacgaag
     2341 gttacagagc aatagcagaa cgggcaagga aaaaatcgcc gcgatagttt agtaaatcct
     2401 ggcttatcca caatgccatg tgcataagct gttcataaca cctgtttact ctggctagac
     2461 aatagctgca tcgaattgcc taaaccatga gctaacgcaa aaaattttat ttttcgcgat
     2521 atcgggatcg tccgcaaagt tacatccaat tcttacacgc tcgacaaaac gcaatgatgg
     2581 cgcctcagcc gaagtctttt cggtgcttag ggaccgtacg ctcggcacgt gagatcctta
     2641 atattctgcg ggcggggttg atggcttcac gaccttcttg acgccatcgc caaccgtatt
     2701 ggccgcatcg cgtgttccct gcccgatttc tttggcaacg tcacgagtgc cgtgaccaat
     2761 ctctcgagtt acatcccgag ttgtgtgacc gattgttcga cccgcttctt taatgtgagc
     2821 acacgccgat acaaaaaata ccgctgcaag aacccataga acggtcttaa aaccatcgtc
     2881 aaccatttgc gcctccgtcg ctgccgctaa tccggagcat tgtatcatag ttggaaaatc
     2941 cgtgatggca gagaactcca gctgcatcct gaggtgtcgg ggtctggcat gaacgcagat
     3001 tcggcaagct aaggaaaata ccttgacgaa aatcaacttg cattgctata taaaaccgca
     3061 attctgcccg gaggaattat caagatacgt ttgttgcgca tcggtgtagt ctcatctttg
     3121 atgatgtgcc tgttgcttgt gagcgcaatc aacctacgtt ccgctcatgc gcaaccaagt
     3181 aacggcaaca tcgtgcgctt tcttgagcag gcaacctttg gcccaactcc gctactgatc
     3241 gcacatcttg agcaggtagg aatcgaggca tatctcaacg cgcagtacac ggctccgatg
     3301 gcggattacc cggagctgga gttttggccg caaatgcgcc cggcgggctg cacaggtgat
     3361 tgtcaacgag acaattacac ctattatcag cttcagcgcc atttcttcac caacgccctg
     3421 tatggtgcgg atcaactgcg ccagagaatt gcttttgctc taagccagat cctcgtaacc
     3481 tcacaggttg acgtcccact gccgagttgg atgcgaacct atcagcaact cctctataaa
     3541 agcgcgttcg gtaacttccg cgaactgctc taccagatta ccgtgaaccc gacgatgggc
     3601 cgtttcctcg atatgttgaa caatcgatgc cagacgcgaa cgccacctga cgtcaatatt
     3661 tgtcgaaacg ggcttaactc tcagcccaat gaaaactatg cgcgcgagat cttgcagctc
     3721 ttctcgatcg gaacattcat gctgaatcaa gacggcacgc ggcaactcga cgccaacggc
     3781 gatccgatcc ccacttacga ccaagagacc gttgaagaat tcgcgcgggt ttttacgggt
     3841 tggatcctcg cgccagcgat cgccgggcct tccagtgccg gcgggacggt gcccaattac
     3901 cgcgacccga tgcgggtgcg gctcgactcg cagggccgcg aggactatca cgataaaggc
     3961 gctaaacaac tgctagcggg ctctcgattg ccagggggcc agtcgcaaag cgccgaactc
     4021 ggcgccgcca tcgacaacat cgcatttcat ccaaatgtcg ctcctttcat aggtaaacag
     4081 ctgatccagc atcttgtaac cagtaaccca agtcctgcct atgtcgcgcg catctccgcc
     4141 gtattcgcgt ccaacgccaa ttcacccacg cagctcttcg aggtggcccg agctattctt
     4201 ctcgatcctg aagcccgggg cgactctgtt gacgctgctg cccagccgaa ttacggcaag
     4261 ttgcgcgagc cagtgcagtt catcaccaat ctcttgcgtg cgttcggcgc caccagcgac
     4321 ggcgttctga atacgctcaa tatcggcggc tcgtcgatcg gatcgggcga catgggtcag
     4381 gatgtattca atgcgccatc ggtgtttagt ttttacccgc cgacggtcag ggtaccggga
     4441 gagaacgcgc tggggccgca gtttgcgctg ttctcttcgt tgacttcgct aaggcgcgcc
     4501 aatttcgcgc agagaattat cttttcaaac attcccgccg ctgctaatcg cccgaccggg
     4561 acatcgatcg atctttcttc atgggattca ttagcgaacg atcctgaccg gctaatcgaa
     4621 gctctgaatc acttattgct ccatggtgtg atgaccgccg aaatgcatga cgccgtgaaa
     4681 gttgccgtgg agagcatccc cgcaagcaac tcgcgcctgc gcgtgcgcac ggccatttac
     4741 ctgatagcca cctcgtcgca gtatcaagtg cagcgctaag aaaggaaaaa acatggcaat
     4801 gacacgccgc aaattcctaa gccggtcttt aggagcgttg ggcgccgcca tgctgggcat
     4861 tgagcgtttt ggtttgctga acgcgcttgc ccaatcggct gattatcgcg cgctggtatg
     4921 cgtctttctc aacggcggca acgactccgg taacatgatc attccctacg acgattatcc
     4981 ggcctatgtg gccgctcgag gatcgagcgg gctcgcgata gagcagtcaa gcttattacg
     5041 aaccggtagc gttccaggca tcggtgcaga atttgggttt catccgagct tgacggccct
     5101 gcacgatctc tgggccgaag gcaaactcgc tgttgcctgc aatgtaggga ctctggtcga
     5161 gccgactacc cgcgatgctt acaggaaccg aacagtccgg gtgccgctca atctcttctc
     5221 ccattccgat cagatcaacc aatggcaaac ctcggtgtca gatagtgcca gcccgtcggg
     5281 gtggggagga
//
LOCUS       DASUVS010000002         8595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1746747, whole genome shotgun sequence.
ACCESSION   DASUVS010000002 DASUVS010000000
VERSION     DASUVS010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8595
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1746747"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VF182_00030"
     CDS             <1..590
                     /locus_tag="VF182_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6435487.1"
                     /translation="EEACARVKKEYPDVAVLKRPSNRPFATFGAHDPEGNYFDLTQEG
                     MENRRDVYVEQAREDPRRIHHIKLRVMNAKAIAAFYRDLFELKEEEKALEDPNYYLTD
                     GKVTLIVAPWKMTDFEGAGIDRPGLEHLGFKVESVDLVKKQLAALRESDPSMRERIVS
                     EPKEGERRLALIASCRHSQHVFSDPDGVFIDVTEN"
     gene            complement(749..2251)
                     /locus_tag="VF182_00035"
     CDS             complement(749..2251)
                     /locus_tag="VF182_00035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX6435488.1"
                     /translation="MQYRNFDHLRTMVRAVSLLLFAVIFFGCTSDIIPATGERRYLGY
                     TWQQETELGKEASKQVAALFGVYRDPKLERYVNEVGNRVLATSHLRRPGAEEQFQKTP
                     VTFTVLDSPIINAMALPGGYIYVTRGMLSHLNNEDELASVLGHEIGHVSARHAARQAW
                     QQQLGQGLLLGGAILGQGLGLPAQEILNVGGMAAQLLFLRYSREDELESDQLSVEYAS
                     LAGYDPAEVISFFQTLNRIQEKEGQGMPNFLSTHPNPGDRVQRIKELTDRRTVERPPQ
                     PTVDRYLNAIEGLVIGEDPRQGFVEGSVFYHPELRFRFPVPRGFKVVNQPTQVVMVES
                     QNRAILGFTSTPEKSVESAAAKFINQPGLRVIQRTPMRSNGLPALLVIGDGQMEKGQM
                     VRLMVYYIEYRGAVYQFLGYSAPQAFSTFQREFMQTMQGFGELQDSRVASKEPVRLAL
                     ERISRQARFQDLMPKNLPAPFTLEDVAIMNQVQLNDEIRPGRTIKLPRAR"
     gene            complement(2464..2688)
                     /locus_tag="VF182_00040"
     CDS             complement(2464..2688)
                     /locus_tag="VF182_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435489.1"
                     /translation="MKKLILLLLLGSLGLAGCEFVAGAATGALATGAGYEINAKRQMD
                     RLDDDYRHERISRRDYEHRRNQIERGSIVY"
     gene            2899..3633
                     /locus_tag="VF182_00045"
     CDS             2899..3633
                     /locus_tag="VF182_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435490.1"
                     /translation="MENPMFWLISLIAGIIIYRLLLRRQLTPKARVTAMLRRYRALEN
                     TGLSERDSLLQLLLTRKDWEKLPRPFLAAIVSRLGSKEDLMRFISLSEDYGYHRDRYP
                     ALAKRIALDTAMAEIACLFSRFGFRLHAQARYKEAEFVQKLALRLQPHAYFTNLPLAA
                     TYYGTGRHAEALPLFKQGFSQLRSFNAGSDLDKHQLAPVNCLTPDEEPEKLWQKYREM
                     YDACLNSTGGTPMAPLTFVMVMELLS"
     gene            3842..4834
                     /locus_tag="VF182_00050"
     CDS             3842..4834
                     /locus_tag="VF182_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435491.1"
                     /translation="MKTRSICLLVLCFVLLLPCSRPAKAAAPHKITMTSGSASERDGA
                     VYVAQDQGFFRKHGVDLSFVQVRNGPVAMSALSSGETQFHWGSVSGANLGAIAEGADL
                     VFVAGFINRLSGMFVVNPKIKTPAELKGKSIGVNSLSGGGWIFSMLMLDYWGLVPERD
                     KIQFRTLGEQAVMAQGVLNGTVDASFLGYTFGKMLEGKGFTVLADSEKLPIPYQGSGL
                     MARRSFVTSSPAAVDSVLRALLDSVAFIRNPDNKNQVTKSLAKALRFRRIEDAEDGYR
                     TMVNLYDRKIYPSVDGIRNVIRLLGANNEKIRRLKAEELIEASAVRRMEKEGRF"
     gene            5073..6176
                     /locus_tag="VF182_00055"
     CDS             5073..6176
                     /locus_tag="VF182_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6435492.1"
                     /translation="MTQFNPIRVGLIGAGGRWGPRAHAPALKGVPETKLYAVCTAHAD
                     TAQAAADKLGVTRAYGDDKQLNADAEVEAVAVAVRVPAHYELTKNAIQAGKHVFCEWP
                     LGANTKEAEELAALARKRDVRTMVGLQRRGSPAYLYLRELIEQDYVGQVLAVNMTLMT
                     SGVLTRTSDRTWQRDVTLGANTLTITFGHVFDAMCMVVGELAEVSALISTQVPQWFET
                     DTKKYVDVTSPDNIAVHGRLDNGAIVNACCGVHPYHGSGHRLEIYGREGTLVMIGGGE
                     GGGERQRKIMGGRKEDKALQELTVPERFKWVPEAVRNDGPAYEVGQMWAKFAEAIRTG
                     SATKTDFDHAVRRHRSLDAIVRASETGQRQKIA"
     gene            6402..7862
                     /locus_tag="VF182_00060"
     CDS             6402..7862
                     /locus_tag="VF182_00060"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6435493.1"
                     /translation="MENRAAEIHDALKHPVIDGDGHWMEPIPIFLEYLREAGGAGAVD
                     EIRALWQRNQAWYRATWPERQHKRLRRTIWWGVTSNTLDKATALLPALLNERLPELGI
                     DFALIYPSFGLSINGIAQDNLHRAAVRAYNMMTADMFAPFAARFAPVAMIPSHTPDEA
                     LEELEYAVRERGFKAIMLRGNEERAIPSAAEGIDRQKAAWYIDTIALDSPYDYEPLWR
                     RCVELGVAVTQHAGSGRWQDRASISNFTYNHVGHFAESNHAFARGVFLGGLARRYPSL
                     NFGFMEGGVSWACQLCLDLIEHWEKRRRDGLQYPGATSIAEMQRLIDRYGDGRLKANA
                     AAIMNNLDAFRPECSLEELGRPEHVADDFEAAGINSKEDVRAVFSNNFYFGCEADDRA
                     TLWAFDPRMGVRLRPVFSSDFTHFDVPDFRQVIPEAFELVEKGFVTEQDFREFTFSNA
                     ARLHTRNNPDFFKGTVVEQAVAHELGLKTPLTAAKG"
     gene            8184..>8595
                     /locus_tag="VF182_00065"
     CDS             8184..>8595
                     /locus_tag="VF182_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX6435494.1"
                     /translation="MKFSDLSIDEIRHQCLDGQKPVSPQILNKLRRDPRQGARKLLEL
                     LKRRYEQERAERIRLQNMLNFERVLWKSGIRTVAGVDEVGVGPLAGPVVAAAVVFPAN
                     TKLDGIDDSKQLEPEQRTNLAARIREHATAIGVGS"
BASE COUNT         1927 a         2349 c         2466 g         1853 t
ORIGIN      
        1 tggaggaagc ctgcgcgcgg gttaaaaagg aataccccga tgtagccgtg ttgaagcgtc
       61 cttccaaccg gccgtttgcc accttcggcg ctcatgatcc cgagggcaat tacttcgatc
      121 tcacccagga aggcatggag aatcgccgcg atgtctacgt cgagcaagct cgcgaggacc
      181 cgagacggat tcatcatatc aagttgcgcg tcatgaatgc gaaggcgatc gcggcctttt
      241 accgcgactt gttcgagtta aaggaagaag aaaaggcgct ggaggatccc aattactatc
      301 tcaccgatgg caaggttacc ttgatcgtcg cgccctggaa gatgaccgac ttcgaaggcg
      361 cgggcatcga tcggcctgga ttagaacact tgggcttcaa agtagaaagc gtcgacttgg
      421 tcaagaaaca actcgccgct ttgcgcgagt cggatccgag catgcgcgaa agaatcgtca
      481 gcgagcctaa ggagggagag cgccgtttgg cgttaatcgc ctcgtgccgc cacagccagc
      541 acgtgttctc ggatcccgac ggagtcttta tcgatgtcac ggagaactaa cacgggatcg
      601 taagcttcat tcaatagccc ggcgcgcctg gggagagggc gcgggtgaaa cgtagaatca
      661 aaactcgccg cgcacactgc ggaccgacca gccttgttcg cgcgacatct gtactcgccc
      721 gggaaaagcc aaaaccgaca acctgctgct aacgcgctct tggtaactta atcgtccttc
      781 ctggtcggat ttcgtcattc aactgaacct gattcatgat cgccacatct tcaagcgtaa
      841 acggtgcggg taagttcttt ggcatcagat cctgaaatcg cgcttgtcgc gaaatacgct
      901 cgagcgcgag gcggaccggt tctttgctcg caacccgcga gtcttggagt tcgccgaacc
      961 cctgcatcgt ttgcataaat tccctctgaa acgtgctgaa ggcttgaggg gcgctgtagc
     1021 cgagaaactg gtaaaccgcg ccgcgatatt caatatagta gaccatcagc ctaaccatct
     1081 ggcctttttc catctggccg tcgccgatca ccaacagtgc gggtaagcca ttggagcgca
     1141 tcggggtccg ctggatgacc cgcaacccgg gctgattaat gaatttagca gcggcggatt
     1201 cgacgctttt ctccggggtc gaagtgaagc cgaggatggc tcggttctgg ctctcgacca
     1261 ttaccacctg cgtcggttga ttgaccactt tgaaaccgcg tggcaccgga aaacggaaac
     1321 gcaattcggg atggtaaaag acgctacctt ccacaaagcc ctgccgcggg tcctcaccga
     1381 tcaccaagcc ttcgatggcg tttaaataac ggtccaccgt tggctgcggc ggtcgttcga
     1441 cagtgcggcg atcggtcaat tctttgatgc gttgaacgcg gtcgcctgga ttggggtgcg
     1501 tggaaaggaa gttgggcatg ccttgtcctt ccttttcttg tatccgattg agcgtctgaa
     1561 agaaggagat cacttctgcc gggtcatagc ccgccagtga agcgtactcg acactcaact
     1621 gatccgattc caactcatcc tcgcggctgt agcgaaggaa caggagctgc gcggccatgc
     1681 cgccaacatt cagaatttcc tgcgccggaa ggccaagccc ctggcctaag atggcgccgc
     1741 cgagcagtag accttggcct aattgttgtt gccatgcctg gcgcgctgcg tggcgcgcgc
     1801 tgacatggcc gatctcgtgc cccaataccg acgcgagctc atcctcgttg ttaaggtgac
     1861 tgagcattcc tcgagtgaca tagatgtacc cgccgggcag agccatggcg tttatgatcg
     1921 ggctgtcgag caccgtaaag gtgaccggtg ttttttgaaa ctgctcctcg gctccgggcc
     1981 ggcgcaggtg gctggttgcg agaacccggt tgccgacctc gttgacgtag cgctccagtt
     2041 taggatcgcg gtaaacaccg aacaatgccg caacttgctt gctggcttcc ttgccaagct
     2101 cggtttcctg ttgccaggta taacccagat agcgcctttc accggttgcg ggaattatgt
     2161 cagacgtgca gccgaaaaag atgaccgcaa acagcaaaag cgaaactgcc ctcaccatcg
     2221 tccgaagatg atcgaaattt ctatactgca tcctcctcct ccttttggct cgcgctgctt
     2281 tccagctcac gatcacaacc ggcgcgcctg cgattcttta gatagacgta gctcgccgtg
     2341 agaccgaagc tccgggcaga gtagattggc cctatgagta atatacactc tccgcgcggc
     2401 gccggaatag aacgcacatt cagatgagaa agaagcagga gaattaggag gcaaccgacg
     2461 aactcaatac acgattgaac ccctctcgat ttgatttctg cgatgttcat aatcgcggcg
     2521 gcttatgcgc tcgtggcggt aatcatcatc gagcctgtcc atctgtcttt tggcattgat
     2581 ttcatatccc gcgccagtgg ccagagcacc ggtagcggct ccagcgacaa actcacagcc
     2641 agccagacct aaacttccga gaagcaacag taagatcagc tttttcatga ttcctcctga
     2701 tctgcttaga ctcatttgct cggtttttgt taacaccggg gaggagtcgt tgccgtagaa
     2761 tgcggcgagc aacgatccga tatagcggat ttatcgaatg atcatcggcg catttgtacc
     2821 accattccaa attccagttc ttttgatctg tcttgacgcg gctgggtgtt gcgtgttttt
     2881 catcaggagg aggtcagaat ggaaaatccg atgttctggc tcatttcttt aattgcggga
     2941 atcattatct accggctatt actccggcgg cagctcactc ccaaggcaag agtcaccgct
     3001 atgctgcggc gctatcgtgc tttggaaaac accgggctct cggaacgcga ttctcttttg
     3061 caacttttat tgactcgcaa ggattgggaa aaactacctc gcccttttct cgcagccatc
     3121 gtctcccggc ttggttccaa ggaagacctg atgcgtttca tttcgctgtc tgaagattac
     3181 ggttaccatc gagaccgtta tccggcgctt gcgaagagaa tcgctttgga cacggcgatg
     3241 gcagagattg cttgtctgtt ttctcgtttt ggtttccggc tgcacgcgca ggcgcggtat
     3301 aaagaggcgg agtttgtgca gaagctcgcg ctacgattac aaccgcacgc ctacttcacg
     3361 aatcttccat tggcggcgac ctattatgga acaggccgcc acgccgaagc tctgccactt
     3421 ttcaaacagg gctttagcca gcttcgaagc tttaacgccg gcagcgattt ggacaaacac
     3481 caattagcgc cggtaaactg tctgacgcct gatgaggaac ccgaaaaact gtggcaaaaa
     3541 taccgcgaaa tgtatgacgc gtgccttaac tcaaccggag gaacacccat ggcgccgctg
     3601 accttcgtaa tggtcatgga gttactatcg tagaggcggg actgcggatt agcatcctct
     3661 ctcgttcgca tgcgctaaat tccacgatac gccttcgggg ctattcccct cgcggccaag
     3721 acggccaagc tgagctgcga gtcttgccgt gaaccgctgg ctgacatcac ttcattcagc
     3781 gcttgcggca gaataaactc tttgccaaga tcggcttcga tcacaaatgg caggtggatt
     3841 catgaaaact cgatcgattt gtcttctcgt tttgtgtttt gttctcctcc ttccttgcag
     3901 ccggcctgct aaagccgctg cgccacacaa aatcaccatg acctcgggat ccgccagcga
     3961 gcgcgatggc gcggtttatg tagctcagga ccagggcttc ttccgcaaac acggcgttga
     4021 cttgagtttc gtccaggttc gtaatgggcc ggtggcgatg tcggcattga gctcgggcga
     4081 aactcaattt cactggggtt cggtttcggg cgctaacctg ggcgcgattg ccgaaggagc
     4141 cgatctggtt tttgtcgcag gatttatcaa tcgcctctca ggcatgttcg tggtcaatcc
     4201 aaaaatcaaa acgcccgcgg agttgaaggg caagagtatc ggggtcaaca gcttgagcgg
     4261 cggcggatgg atcttctcga tgttgatgct ggattactgg ggattggtcc cggagcgtga
     4321 caaaattcaa tttagaacgt tgggcgagca ggccgtgatg gctcagggcg tgctcaacgg
     4381 caccgtcgat gcgtcgtttc ttgggtacac cttcggcaag atgctcgagg gcaaggggtt
     4441 caccgtcctc gccgacagcg aaaaattacc aataccctat cagggctcag ggctgatggc
     4501 gcgacggagc tttgtcacat cgtctcccgc cgcagtcgac agtgtgctca gagcgttact
     4561 ggatagcgtc gcttttatcc gcaatcctga taacaagaac caggttacca agagtctcgc
     4621 caaagcgctg cgtttcagac gcatcgaaga tgccgaagat ggctatcgca cgatggtcaa
     4681 cctttacgac cgtaagattt atcccagtgt cgacggcatt cgtaacgtca tccgtctact
     4741 gggggccaac aacgagaaga ttcgccgcct taaagcggaa gagttaatcg aggccagcgc
     4801 ggtgcgcagg atggagaagg aaggacggtt ttagcggttg gcattatcgt tatcgtggtt
     4861 agccaagaaa ttcgatgcga ttgtctggct cgtcctgtgt ttcgctgccg agctacaact
     4921 cacgctatct tgaagtttag caacgtaacg cgcgcgtcta gctcagactt ttttctaagt
     4981 ttggtataac gtaagagtct gaactggcgc tgtgacatct ttgcggtttg cgttgcgttg
     5041 cgccgataat ttcaatttac aagagggaaa cgatgaccca attcaatcca atccgcgtcg
     5101 gactcattgg agcagggggg agatgggggc cgcgggcgca tgcgccggca ttaaagggcg
     5161 tgcccgaaac caagctctat gccgtgtgca cggcgcatgc ggataccgcg caggcagcgg
     5221 ccgataaatt gggcgtgacg cgcgcttacg gcgacgataa acagctaaac gccgatgccg
     5281 aagtcgaagc ggtggcggtc gcagtgcgcg tcccggcgca ctatgagctt acgaaaaacg
     5341 ccattcaagc aggcaagcac gttttctgcg aatggcctct aggcgcaaat accaaagagg
     5401 ccgaagagct tgcggcgctg gcgcgcaagc gagacgttcg caccatggtc ggcttgcaac
     5461 gtcgcgggtc gcccgcttat ctgtacctgc gcgagctgat cgagcaagat tacgtcggac
     5521 aggtgctggc ggtgaacatg acgctcatga ccagcggcgt gttgacccgc acctccgatc
     5581 ggacctggca gcgcgacgtc actctaggcg caaacacgct gacaatcaca ttcggccacg
     5641 tgtttgacgc catgtgcatg gtggtgggcg agcttgctga agtctctgcc ttgatcagca
     5701 cgcaggtccc gcaatggttc gagaccgaca ccaaaaaata tgtggacgtc acctcacccg
     5761 acaacatcgc ggttcacggc cgcttggaca acggcgccat cgtcaatgcc tgctgcggcg
     5821 tgcatccgta tcatggcagc gggcacaggc tggagatcta tggcagggag ggcacactgg
     5881 tgatgatcgg cggtggcgag ggtggcgggg agcggcagcg taagatcatg ggcggacgga
     5941 aagaggataa ggcgctccag gaattgacgg tgccggagcg attcaagtgg gtgccggaag
     6001 ccgtgcgcaa cgatggtccg gcctacgaag tcggacagat gtgggccaag tttgccgagg
     6061 cgatacgcac cggctcagct acgaaaaccg acttcgatca cgcagtgcgc cggcaccggt
     6121 ccttggacgc gatcgtacgc gcttcagaga cgggccaacg acagaagatt gcctaacggc
     6181 acagttcgag acgttccaaa agttccaatc gttccacttg ttcaagaggg tttagacgtt
     6241 ccagtgcttt ttgctcacaa caagtccatt cgattggtta tgaaacgatt ggaacaattt
     6301 gaacggagcg cagcggtgga acgttggaac tattctcaac gattgacgac ttcctggcca
     6361 gccgctaaag agaaaagagc ctagcaagag gagcatcgac catggaaaat cgcgcggcag
     6421 aaatacacga tgcgctcaag catccggtga tcgacggcga tggccactgg atggagccga
     6481 ttccgatctt tctcgaatac ctgcgcgaag cgggcggcgc tggagctgtc gatgaaatca
     6541 gagcgttgtg gcagcgcaat caagcctggt atcgagccac ctggccggaa cgccagcaca
     6601 aacgcctgcg ccgtacgatt tggtggggcg tcaccagcaa tacgctcgac aaagccaccg
     6661 cgctgctgcc ggctttgctc aatgagcgtc tgcccgaatt gggcatcgat ttcgcgctga
     6721 tttatcctag cttcggactg agcattaacg gcattgccca ggacaacctg cacagggctg
     6781 cggtccgcgc ctacaacatg atgacagctg atatgttcgc cccgttcgcc gcaagatttg
     6841 cgccggtagc gatgatcccg tcccatacgc cggacgaagc tctcgaagag ttggagtacg
     6901 ctgtccgaga gcgagggttc aaagcgatca tgttgcgggg aaacgaggaa cgagcgatcc
     6961 caagcgcagc ggagggaatc gaccggcaga aggccgcctg gtacatcgac accatcgctc
     7021 tggacagccc ctacgactat gagccgctct ggcggcgctg cgtcgagctc ggcgtggccg
     7081 tgacccaaca cgccggcagc ggccgctggc aggatcgcgc ctcgatcagc aacttcacct
     7141 acaatcacgt cggccacttc gccgaatcaa accacgcctt cgcccgcggt gtctttctgg
     7201 gtgggctggc gcgccgctat ccgagcctca atttcggctt catggaaggc ggcgtaagct
     7261 gggcctgtca gttgtgtctc gacttgatcg aacactggga gaagcgccgt cgcgacgggt
     7321 tgcaataccc gggcgcgacc agcatcgctg agatgcagcg gctgatcgac cggtatggcg
     7381 acggacgact aaaagccaac gcggccgcga tcatgaacaa cttggacgca ttccggccgg
     7441 agtgcagcct cgaagagctg ggccggcccg agcatgttgc cgatgatttt gaagccgcag
     7501 gaatcaactc aaaggaggat gtccgcgcgg tattctctaa caacttttac ttcggctgcg
     7561 aggcggacga tcgcgcgacg ctttgggcct tcgatccgcg catgggcgtg cgccttcgac
     7621 cggtgttcag ctccgacttc acgcatttcg acgtgcccga tttcaggcaa gtgattcctg
     7681 aagcgttcga gctggtcgaa aagggcttcg tgaccgagca ggatttccgg gagtttacgt
     7741 ttagtaacgc cgcgcgcctg cacacgcgca acaatccgga ttttttcaag gggacggtcg
     7801 ttgagcaagc ggtggcccat gagcttggac tcaagacgcc gttgacagca gcgaaaggct
     7861 aagagactgt actatcatcg aatcatgagg actggagtga tggagtcatc tctgatggac
     7921 ctagaccgaa actcaattcc aataggacga atcattttgt ggccgtctga tcgattgaca
     7981 gccccgaaaa tcactattca gcaaacggct catcggagta tttgaatccg gttacgttga
     8041 tttccggaat caaatagttt acttccgcaa gacatgccgg agtgtgccac aaggtgtccg
     8101 cgggcagaac tgaaaccttc gttgcgcatc ggcattaaaa tctttggatc aactagagtt
     8161 cgatctcaga gcaattcccg gcgatgaaat tcagcgattt atcgatcgac gagatccggc
     8221 atcaatgtct cgatgggcag aagccagtca gtccgcagat tctcaacaaa ttgcgtcggg
     8281 atccgcgcca aggcgcgcgc aagctcctcg agcttctcaa aagacgctac gagcaagagc
     8341 gcgccgagcg aatacggctg caaaacatgc taaatttcga gcgagtactg tggaagtccg
     8401 gcatccgcac ggttgcgggg gtggacgaag tcggagtcgg accgttggcc gggccggtgg
     8461 tcgccgcggc ggtggttttt ccagccaaca caaagcttga cggaatcgat gattccaagc
     8521 agctcgaacc ggaacagcga actaatttgg cggcgaggat tcgtgaacat gcaaccgcta
     8581 tcggagtggg ctccg
//
LOCUS       DASUVS010000003         4770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1213, whole genome shotgun sequence.
ACCESSION   DASUVS010000003 DASUVS010000000
VERSION     DASUVS010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VF182_00070"
     CDS             complement(<1..717)
                     /locus_tag="VF182_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6435495.1"
                     /translation="MPRPRIIEWPEQARICVTFIIPWEVWPENFATRESLQRHGTHTI
                     PPVNAVFKQNLAAVTEREYGDRVGIWRIMDMFDRHGLKVTFLMNGLKVEQFPEQCREF
                     NSHGHEFSSESYEHEYSFMYTREQERESIQKTVAAFEKVLGQKPTGYLSPGHASTPHT
                     LELVAEAGFIWWADPLNSDNPYSVDSKGRKVVVVPYNVPGCNDYSTYGAGRTPRDLLQ
                     IMKDQFDYLYWEGEQGAPKFF"
     gene            complement(1039..1623)
                     /locus_tag="VF182_00075"
     CDS             complement(1039..1623)
                     /locus_tag="VF182_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX6435496.1"
                     /translation="MEHANSRDFSPTDQVEKPTEILSNEHRIIERVLSAVEKLAKGPV
                     AALEPWKKSLDFFRHFADECHHFKEEELLFPLMEEHGIPIEGGPIGMMLVEHEEGRSY
                     VRSMLAALSLVEAGNESAKESLVSSAQAYCRLLREHIEKEDEILFRMADEVISADQQK
                     KLLALFAEHEAKQVGAGIHKKYLDVVLELEGTVA"
     gene            complement(1654..2871)
                     /locus_tag="VF182_00080"
     CDS             complement(1654..2871)
                     /locus_tag="VF182_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04053 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HEX6435497.1"
                     /translation="MADVACIVLPPSTMDFNQRPFTVVWETTRACDLACVHCRAEANS
                     QRSAGELTFDEAQTLIQGIKAFGAPYPLFILTGGDPAKRSDIFDIIAYAHGEGLRVAM
                     TPSATPLITRDAVRRFAAAGLVRLALSLDGKDPLTHDSFRRVAGSFDRTLQILDWCRE
                     FGLETQVHTTVTRHVLDELPAIAETIGERGIKLWALFLLIAVGRAARPEVRRLNLSAR
                     QFESLFHWLYDLDKSASFDVTPREGYHYRRVLLQRRAAEAGITVENLLREVEKKHSTP
                     ADVRTSPKATRVLRAPLGVNDGKGIAFISHRGDVQPSGFLNLVGGNIRREPLTSIYRN
                     SPVFLRLRDSSLIKGKCGVCEFKNICGGSRSRAYAITGNPMHSDPFCAYQPAAWRAAA
                     NVKAEREEQPASI"
     gene            complement(2990..4087)
                     /locus_tag="VF182_00085"
     CDS             complement(2990..4087)
                     /locus_tag="VF182_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435498.1"
                     /translation="MKVNPKCGRFVLMGLAGLSLLAGLWAGIARMGWFLPVPNSQFIV
                     IHGPLMVVGFLGTLIGLERAVALQRWWPYGIPVLAGLSALAGLAGGPPELTASLAAVA
                     SILMVSVFLTLYRQYPSEHFIIMALSAVAWLVGNVLWFTEQAIFVFVPWWVGFLVLMI
                     AGERLELSRLRRPTPLIRGAFHASVAVILIGLVCSYFDFPTALRLVGVGLLTLALWLL
                     RYDLVWQSARQPGLPRFMATSLIAGYVWLAVGGILWMVFAQFFSAGPRYDAMLHAIFL
                     GFVFSMIFAHAPIILPTITGLALPFQNIFYLHAGLLHLSLLLRIAGDLSPAPSLQQWA
                     GLLNAGAILLFLLNNVRAVRLGASARSIRKI"
     gene            complement(4519..>4770)
                     /locus_tag="VF182_00090"
     CDS             complement(4519..>4770)
                     /locus_tag="VF182_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEX6435499.1"
                     /translation="VFWRGSFGRTLAIKLILVAAILGISGFHDFFLGPRASAAWESNP
                     ASAETVRLRRQAVKLGRLNLLLALTAIVLGVMLVRGVPW"
BASE COUNT         1129 a         1356 c         1304 g          981 t
ORIGIN      
        1 aaagaacttg ggcgcaccct gctcgccttc ccagtagaga taatcgaact ggtctttcat
       61 gatctgcaag agatctctgg gcgttcgtcc cgcgccatag gtcgagtagt cattacagcc
      121 cggaacattg tagggaacaa cgacgacttt tcgccctttg gaatccaccg agtaggggtt
      181 gtcggaattg agcggatcgg cccaccagat aaagcccgcc tccgctacca attccagagt
      241 atgcggtgtc gaagcatgcc ccggtgacag gtagccggtc ggcttctgcc ccagaacctt
      301 ttcaaaagcg gcgacggttt tttgaatcga ctcccgctct tgctcgcgcg tgtacataaa
      361 agaatactca tgctcatagc tttcagagga gaactcgtgt ccgtgagaat tgaactctct
      421 gcactgctcg ggaaactgct cgaccttcag gccgttcatt aaaaaggtga ctttcagacc
      481 gtggcggtcg aacatatcca tgatgcgcca gatgccaaca cgatcgccgt attcccgttc
      541 ggtgactgcg gcgagatttt gcttaaatac cgcattcacg ggtgggatcg tgtgcgtgcc
      601 gtgacgctgt aacgactcgc gtgtggcgaa attctctggc cagacttccc atgggatgat
      661 gaacgtaacg caaattctgg cttgctcggg ccattcgatg attctcggtc gcggcataaa
      721 gcctcctaca tgaccttttg gcgtattagt gtgactggtt cccggtgatt tccgtgacgc
      781 tcattgcggc gcagggcagg cgcaggtgct atgtcaaaga ctgcgaatta ccgttacacg
      841 gtatcggcaa gagcgcctat cgccagagcc caaagatcaa gccttccggg aacgggagat
      901 gaaggagcct gttggaaaag ccttccacgg aaagcatgaa attgcagtta agacaatcgt
      961 gagcatccac ggttctccgc gggccaattc aaataaccta gaacaaagat tggtggcgga
     1021 taaacacccg aagcggcctt atgccaccgt gccttccaat tccagcacga cgtctaaata
     1081 ttttttatgg attcccgcgc ctacttgttt ggcctcgtgc tcagcgaaaa gcgccaaaag
     1141 ctttttctgt tggtcggctg aaatcacttc gtccgccatg cgaaagagga tctcgtcttc
     1201 tttttcaata tgctcgcgca gtagacggca gtaggcttga gcgctgctga caagactctc
     1261 tttagcagac tcgttgcctg cttccaccaa cgagagcgcc gcgagcatgg accgcacata
     1321 ggatcttcct tcctcgtgtt ccaccagcat catgccgatg ggcccgccct ctatgggaat
     1381 accgtgctcc tccataagag gaaacaacag ctcttcctcc ttgaagtgat ggcactcatc
     1441 ggcgaagtgg cggaagaaat caagcgactt cttccaaggc tccaatgcgg ccaccggtcc
     1501 tttcgcaagc ttttctaccg cgctgagcac gcgctcgata atgcggtgct cattgctgag
     1561 aatctctgtt ggcttctcca cctgatcggt tgggctaaaa tcccgcgaat ttgcgtgttc
     1621 catctgatca tctccttagg ggtacgtcgg tctctatatg cttgccggct gctcttcacg
     1681 ttcggctttg acgttggccg cggcccgcca agcagcaggc tggtaagcac agaatggatc
     1741 gctatgcatc ggattgccgg tgattgcgta ggcccgcgat cggctgccgc cgcagatgtt
     1801 cttgaactca catacgccgc acttgccttt gatcagagag gagtcgcgca agcgcaggaa
     1861 tacgggcgaa ttccgataga tgctagttaa cggttcgcgc cggatattgc cgccgacgag
     1921 attgagaaaa ccgctcggtt gcacatctcc tcgatgcgat atgaaagcga tgcccttgcc
     1981 atcattcacg ccaagcggag cgcgaaggac acgggtcgct ttcggggagg tcctaacatc
     2041 tgccggtgtc gaatgctttt tttccacctc gcggagcaaa ttctccacgg tgatgcccgc
     2101 ctcggccgcc cgtctttgca gcagaacgcg gcggtagtga tagccttccc gcggtgtgac
     2161 atcgaatgac gcggacttat ccaggtcata gagccaatgg aacaacgatt cgaactggcg
     2221 cgcgctgaga ttgaggcggc gcacttccgg ccgcgccgcc ctgcccaccg cgatcaagag
     2281 aaaaagcgcc cacagcttga tgccgcgttc cccgatcgtt tcggcgattg cgggtagctc
     2341 gtccagcacg tgacgggtga ctgtggtgtg aacctgcgtt tccagaccga actctcgaca
     2401 ccagtcaagg atttgcaggg tgcgatcaaa cgagcccgcg acacggcgaa aactatcgtg
     2461 ggtcagcgga tccttgccat ccaagctgag cgccaagcga accagcccgg cagcggcaaa
     2521 acgcctgact gcgtcccggg taatcagcgg cgtcgccgat ggcgtcattg cgacacgaag
     2581 cccttcccca tgcgcatagg cgatgatgtc gaaaatatct gagcgcttgg ccggatcgcc
     2641 gccggtcaga ataaatagcg gatacggcgc gccgaatgct ttgatgcctt gtataagtgt
     2701 ttgcgcttcg tcgaacgtaa gctctccggc gcttctttga gaattcgctt cggcacggca
     2761 atgcacgcag gcaagatcgc aggcgcgagt ggtctcccaa accacggtga agggacgctg
     2821 gttaaaatcc attgtcgaag gaggcagcac gatacatgcc acgtcagcca tgcggaagat
     2881 acacgctagg ggagcgctct ccggcggctt gctgaagcag ccggtttgtt atgaccggga
     2941 ataaagcgcg agccgtttct tgtttccgag aaatcggctg ctgagggatt tagatttttc
     3001 gaatcgaacg agcgcttgcg cccaagcgca ccgcgcgcac attgttgagc aagaaaagta
     3061 agatcgcgcc ggcattgagc aaaccagccc attgttgcag agaaggcgct ggtgaaagat
     3121 ctccggcgat tcgtaaaagc agtgataggt gcaataaacc ggcgtgcaga taaaaaatat
     3181 tttggaacgg caaggctagt ccagtgatcg tcggcaatat gatcggcgcg tgggcaaaga
     3241 tcatcgagaa cacgaaaccc aaaaagattg cgtgcaacat cgcgtcataa cgtgggccgg
     3301 cgctgaagaa ttgcgcaaac accatccaga gaattcctcc taccgccaac cacacgtagc
     3361 cggcgatcag agacgtcgcc atgaaacgcg gcaagcctgg ttgacgggcg ctctgccaga
     3421 ccagatcgta acgcagcagc cagagcgcca gcgtaagcag cccgacgccg accagccgca
     3481 gagccgtagg aaaatcgaaa taggaacaga caagtccaat gagtatgact gccacagagg
     3541 cgtgaaaggc gccacgaatc aacggcgtag gtcgccggag gcgcgacagc tccagtcttt
     3601 cacccgcgat catgagcacc agaaatccga cccaccaggg gacaaacacg aagatcgcct
     3661 gttcggtaaa ccagagcaca ttgccaacca accaggcaac ggcgctcagc gccatgatga
     3721 tgaaatgctc ggaagggtat tgacgataga gcgtgaggaa caccgaaacc atgagtatgc
     3781 tcgctaccgc ggccagtgac gcggttagtt cgggcggtcc accggccagc cctgcaaggg
     3841 cgctcaaacc ggccaacacc ggaatgccgt agggccacca gcgctgcaac gccaccgccc
     3901 gctcgaggcc gatcaaggtg ccgagaaaac cgaccaccat cagcggtccg tggattacta
     3961 taaattgact gttcggcacc ggcaggaacc agcccatgcg agcgatgccg gcccagaggc
     4021 cggctagcag tgagagccca gctaaaccca tgaggacgaa gcggccgcat tttggattta
     4081 ccttcatcgt tcaactaacc ctaatccgac atcattctaa gcccgcgagg ccagcgaggc
     4141 ctacagccgt gggcaatggg atagtgcctt gaaggccgcc ccgcattccc ctaatggtga
     4201 tgtttcgtac gtcgcggcct tagagtcaat tgcgcgttcg agtgcccacg gcttccggtc
     4261 tcgcggtcct cgctcactgc tgacgatgtc aactattgtc gggttagggc caactttacg
     4321 ctgacggcga gtcagaataa atccgaaatc cgaatatcga aatccgaaac aaacttaaaa
     4381 ttccaagcgc gataaagatt tctcggattc accgcgaagt gacagttacg ttacacgccg
     4441 tggactggcc catttgtttg catcattcgg ctgttcattc gaaattgttt cgaatttcga
     4501 gctcgtaatt ccattgctct accagggcac gccgcgcacc agcatgacgc ccaggacaat
     4561 tgcggttaat gccagcagca gattgagccg gcccaatttt accgcttggc gcctcagtcg
     4621 aactgtctcg gctgaagcgg gattagattc ccacgccgca gacgctcgag gccctaaaaa
     4681 aaaatcgtga aaaccactga tccccagaat cgccgcgacg agtatcagct ttatagcaag
     4741 cgtgcgtcca aaagagcctc gccaaaaaac
//
LOCUS       DASUVS010000004        13492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_438284, whole genome shotgun sequence.
ACCESSION   DASUVS010000004 DASUVS010000000
VERSION     DASUVS010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13492
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438284"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..811)
                     /locus_tag="VF182_00095"
     CDS             complement(263..811)
                     /locus_tag="VF182_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6435500.1"
                     /translation="MRVPEITNREDLPEDQREFYDSIMASRKRIGAPYNFLLHAPDLA
                     ARTAHLIGYALFNTDFPEDVKELAICTVAREMDCSFEWAAHAEDALKAGVRQECVEII
                     QNRKAPAGLSEREAEVVRYVQELLRPPHRITPATFDALRKRIGVPRLVELTGIIGAYV
                     GLACSLNAFAVEAPPGQPILPF"
     gene            complement(1092..2294)
                     /locus_tag="VF182_00100"
     CDS             complement(1092..2294)
                     /locus_tag="VF182_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6435501.1"
                     /translation="MMTSAQVFTAVDQVASDEEIDRLLVDLLRYPSPQTERLEDDPQL
                     KKFIAQMVAPRLAELTGSAGAIDAMGNLIWRLGDASAETGKGLLLMGYAMTFPAGSMK
                     QPFSGALVDGTVFGAATQCALGRGACEQKGALAAMIYAAAIVARTQLSLRAPLILAVS
                     LAGETGRHDAVQFILENDAISAHSAIIGLATGNRICLGNKGRIDIEIIVRGKSAHSSM
                     PEQGINAVDGFRRVLERLDRLPLKAAHHGLGNATVTVTQVTSGPNISHTIPDVCRAVL
                     DRRLLPGEDPDAAFREIQEALKDLRAWSITFTRGAFMYPSEIGRDAAIVAAIDRATRE
                     LTKKESELFYSPAALDAGFLNRNGIETVMFGPGDLRLAHTDQEAVPLQEIRDAARIYA
                     ATALQLLG"
     gene            complement(2317..3489)
                     /locus_tag="VF182_00105"
     CDS             complement(2317..3489)
                     /locus_tag="VF182_00105"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX6435502.1"
                     /translation="MAAATFVFNNPRVERIVSGPGSIEKLSGEIGRLEGTRAMIVISP
                     SVAKTLLLDRIRAELGAKCVAVFDEVKPHSPTESIAKAVESARRARIDVLVSAGGGSA
                     IDTAKGVAALLAEGGSLPRLSVRFTPPNEKEVPPMPAAKLPHIAIPTTLSGGEYSYSA
                     GISEGGKKYIIADPKLAPRVVLLDPEAAATAPARLLAASGMNALAHCVEAMYSTETQP
                     LTDAYCLRGIGLIARDLPRAVENSRDLEALSQVQVAACLSGMGVYSAWTGIHHAIVHV
                     IGGRYKAPHAEIHALMLPYAMRWNLDATVEAHARMAREIGIEGADENALAAAVPEHVF
                     TLNRRMDLPLRLRELGVPREGLRQLASDALDDYSIHTNPKPVTAAEQVLEVLEQAW"
     gene            complement(3499..4557)
                     /locus_tag="VF182_00110"
     CDS             complement(3499..4557)
                     /locus_tag="VF182_00110"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6435503.1"
                     /translation="MPVKILKSLLVRFRGLTTACAVLLIALGVNDQPAHGAAPFYEGK
                     TIRIIVGTSPGGGYDTYTRLIARHFSNHIPGKPAIIVDNMPGAGGLVSANHLFKVAKP
                     DGLTIGHFVGGQFLQQLLGKPGIEFDALKFEYVGVPAQDNFVFGVSKTTGITDIEQWL
                     ASKTAVKFGAIAAGDGTYDIPKVAEIALGLPIQVVSGYKGTAPIRLAFNSDEVGGLSN
                     SWQSFRSTWRKELDNGEVIIVLQVSAKPHPDLPKVPLTMSIAKTDEARKLMQAVAQAH
                     GAAVRPYVLPPGTPKDRVEILRTAFMETVKDPELLKEAAKANLEINPGSGEDLERNVK
                     ELLRLDPPLVARLREILK"
     gene            4855..5592
                     /locus_tag="VF182_00115"
     CDS             4855..5592
                     /locus_tag="VF182_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX6435504.1"
                     /translation="MKLAELHWPEVEKLDREKIVALIPVGSMEQHGPHLPFQVDVFCA
                     SRLAEDLEKKIPDVLLVPPLWAGVSAHHMDFPGSITLRAKVFIDVLHDICGSLHHHGF
                     RRIVLLNGHGGNRSSLEVLGQELFVEFGLTANTLAYWDLVPDLVKSLKKTQSIGMGHS
                     GELETSLMLYLAPELVDRQAIPAGVLGVEPPGPTSGIKRYVNMKEHSAAGVIGMPSAA
                     APEIGKELYQGALNALEKAVRVLQSDH"
     gene            complement(5610..6644)
                     /locus_tag="VF182_00120"
     CDS             complement(5610..6644)
                     /locus_tag="VF182_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6435505.1"
                     /translation="MKKSTLHRRLILVCAMFSPFFLVLCRSANGQETALIEAARKEGK
                     VTIFGSLQDEVMKEIQGSFDKKYPGVKSVYWRASTTAVMDRAINEFRTGKVSWDIFFT
                     GVDAMEIMRNEGMFLKYQTPAARNFDPSYHHDFYSPNYRSSIIGFVYNTRYIKPSDAP
                     KSYWDYVDPKWDGKTTMSDPTSHTSMAKWLSSLHLVLGDRSKEDEYAQRLAASRPLLH
                     RSLTPAVESIANGEKPLGIAYIKYVCDMGKAGAPLDYVRLPAYLGESNYIAIGSKSAN
                     VNAAKLWIDHWYSRDSMEALAKDCEFVNLRGVHPPLKDADKIKFVEEIQRSEGDYKQL
                     GQKYAKFFKK"
     gene            complement(7565..8059)
                     /locus_tag="VF182_00125"
     CDS             complement(7565..8059)
                     /locus_tag="VF182_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435506.1"
                     /translation="MQNGKTLIWLAIGFLSALMLSDSALADSGRSHREGRSAIRESHR
                     DVQKNRAELRQDFAELRRDRAELRSDIRRGAPRHEIARGKSEIRRDVREIERDRRELR
                     RDQSELRSNLRKSGYDNHWYRGGNQRMYGTWNDRDRWDRRRSGWFNSRDSRYSWWNND
                     RFGR"
     gene            complement(8614..9360)
                     /locus_tag="VF182_00130"
     CDS             complement(8614..9360)
                     /locus_tag="VF182_00130"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6435507.1"
                     /translation="MDRATFREQLLVVMEGKDHWAWPAFTSGQVPKDLLHHHLEQEYA
                     TYVRDFPVMVGRAYVRCPIALVRRSLAENLYEEETGGLVAGSPHPELFLEYARGLGMD
                     LKRFANVSLLPAAKNYRQYLDNATQHYGWEIAAAVVTIFVEGSKDERRAIESTVAVPP
                     PPLDEHPLVKHYGLPIQHLALTKAHRQVEGNHRAAAWAAILDHVKPTRRGTVVRAMQE
                     ALAGWLSYRDAVAEICGVDCNGHNYKTAIA"
     gene            complement(9617..10969)
                     /gene="gor"
                     /locus_tag="VF182_00135"
     CDS             complement(9617..10969)
                     /gene="gor"
                     /locus_tag="VF182_00135"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEX6435508.1"
                     /translation="MAQYDYDLFTIGAGSGGVRGSRVAASFGAKVAVAEESALGGTCV
                     NVGCIPKKLLVYASHYSDDFSDAAGFGWTTGPQSLDWAKLIANKDSEIYRLNGVYRKL
                     LDDAGVQIIEGHAQVVDPHTVAVDGKRYTTKYILIAVGSWPSIPIFPGSQHVITSNEA
                     FYLPELPDKVVVVGGGYIGVEFAGIFHGLGAKTAQLYRGELFLRGFDNDIRLTLADEM
                     LKRGIDLRFNTGIANIEKHGEILRATLIDGSVIDANQILYATGRVPKTRRLGLENAGV
                     ELRQSGAVVVDPYLRSSVESIYAIGDCTDRLMLTPVAIAEGTAVANTLFNNRPIMLDY
                     ENVATAIFSTPNCGTVGLTEEQARRREFEVDVYMGTFRPLRHTMTGSSEKTMMKLVVD
                     HVSDRVLGCHMVGANAGEIIQGFAVALNCGATKTQFDTTIGIHPTAAEEFVTMRTKTR
                     "
     gene            11079..12368
                     /locus_tag="VF182_00140"
     CDS             11079..12368
                     /locus_tag="VF182_00140"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6435509.1"
                     /translation="MPSLARSLFLPTMPPSLFRSTSTGSRRLLAAVSALRHRNYRLYW
                     FGQLFSVLAQNMEAVAQSWLVLEITNSPLLLGLTGLTFSIPTIALTLLGGAVADRTDR
                     KRIMICSQIGSFVMFLLLAILVVNELVVLWHVMTAAFFSGCFRAFDRPSRMALLPLMV
                     PKQDIPNAVAIGGTIWQLNKLVGPALAGMLIYLVSIGATYLFCCAASGTAILLWLGIR
                     LDRQRITATAGGLLQNMLDGLNFIRKNEIYYSFIGMTFFNSVFGMSYLILMPVFARDI
                     LFVGSEGFGLLQSAGGAGALCGVLLVAYFGQARGKGKQALSGALVFGMLLIVFAYSNS
                     YRLSLAMACVLGMASQFYMTTIHAILQVNLPDQLRGRVMGIHGLAWELMPVGGMIGGV
                     IAEVNGAPSAVAFGGAMVAGMALVVAVFLPSIRRLEQ"
     gene            12403..13386
                     /locus_tag="VF182_00145"
     CDS             12403..13386
                     /locus_tag="VF182_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEX6435510.1"
                     /translation="MAEKKAKKKSGTARKRRKLAGKSTGLTPTELVGIAPAVIESLER
                     AVEEDGGRVISSYREPFGGQWLIMASLPIQQVEPTPYQRNLSDTHVRKLETVIAKLGR
                     FLDPIIVVRGEPNQDGVRYWTPNGNHRLSALRTLGAKSVTAIVVPETSTAYKILALNT
                     EKSHNLRERAMEVIKMYQELAMLDGADEESYALEFEDAALITLGLCYLEQGRFSGGAY
                     HPILRRVDGFLRKPLQAALEIRRERSKMLMDLDKKVCERVDMLKKRGLTSPYLKSFVV
                     ARINPFRFRPKDVEPLSYNEVLERMIKAVDKFSPEKIRMEDLAKSGGAPEE"
BASE COUNT         2961 a         3787 c         3648 g         3096 t
ORIGIN      
        1 atcgtgacga agatatatga agtccctatg aaaggagtgc aggaggatcg cagcggttca
       61 aaagttcaac cgttcaatgt tcaacgactg ataacaactt cgatgttgaa attccaaaac
      121 ttcgaaacga cactcagcaa cttgacgtac cggaattgat ttttaaatgt aggtttattg
      181 gcttggctgg ttagctgaga ataatcagcg aatacatcga ggctcaaagc cttgctcagt
      241 ccctccagct cgcagcgatt tcctagaacg gcagaatcgg ttggcccggc ggcgcttcca
      301 ccgcgaaggc attgagcgag catgcaagcc ccacataggc gccgatgatt cccgtcagct
      361 cgactaatcg aggaacaccg atgcgttttc ttaacgcatc gaacgttgcc ggcgttatcc
      421 ggtgcggcgg ccggagaagc tcctggacgt aacgcaccac ctctgcctcg cgttcactga
      481 gtccggccgg cgccttgcga ttttggatga tttcgacgca ttcctgccga actcccgctt
      541 tgagcgcgtc ctccgcatgc gcggcccact cgaacgaaca gtccatctcc cgggccacag
      601 tacagatcgc cagctccttt acgtcctcgg gaaagtccgt gttgaaaagc gcatagccga
      661 tcaggtgcgc ggtccgcgcc gccaggtcgg gagcatgaag caggaaatta tagggagcgc
      721 cgatccgctt tcggctggcc ataatcgaat cgtaaaattc tcgctggtct tccggaaggt
      781 cttctcggtt agtgatctcg ggtactctca cgatcgcctc ctgcgaaggt tttcatcccg
      841 cgaaatgtgt cgctcagcac cctcgaatgg tctcgcattt tgatcgaatc gaaccttcac
      901 tcgggttcga gttcggttca acagtgcgct ccgttcaaac gtccgaacta gagaacacgg
      961 agatttcgcg cgccagacgc taagcacccc gggtcggata aacatcgttc gtcttcgttg
     1021 cctctgcgtt tttgcgcgag gcattcggac cttcgtgtcc ttcgcgcctt cgtggtgagt
     1081 ccgtattctc gctatccgag cagctgcagc gcagtcgcag cataaatcct cgccgcgtca
     1141 cgaatctctt gcagcgggac ggcctcttga tccgtgtgtg ccaagcgcaa atcgccggga
     1201 ccgaacataa ccgtttcgat gccgttgcgg ttgagaaatc cggcatccag agcggcgggc
     1261 gaataaaaca attccgattc cttcttggtc aactcgcgag tggcgcgatc gattgccgcc
     1321 acgatggcgg cgtctctgcc gatttccgag ggatacatga aagcgccgcg tgtgaacgtg
     1381 atggaccagg cgcgcaaatc cttgagcgct tcctgaattt ctcgaaatgc cgcatcagga
     1441 tcctcaccgg gtaacagcct gcgatcgagc acggcgcggc aaacatccgg gatcgtatgg
     1501 cttatattgg gtccgctggt aacttgtgtc accgtcaccg tggcgttgcc gagaccatga
     1561 tgagcggcct tgagcggcag tcgatccaac cgttccagca cccggcgaaa tccgtccacg
     1621 gcgttgatgc cttgttcggg catgctgctg tgggccgact tgccgcgcac gataatctcg
     1681 atatcgatcc gccccttgtt gccgagacag attcgattcc ccgttgccag accgatgatg
     1741 gcagagtgtg cgctgatcgc atcgttttcc agaataaatt gtaccgcgtc gtgacggccg
     1801 gtctcgcccg ccaagctcac cgcgagaatt agcggcgcgc gcaagcttaa ttgtgttcgc
     1861 gctacaatcg ccgcggcgta gatcattgcc gccagtgctc ccttttgttc gcatgcgccc
     1921 cggccgagag cgcactgcgt cgccgcgccg aacaccgttc cgtcgacgag cgcgccggag
     1981 aacggttgtt tcatcgaacc cgcaggaaag gtcatggcgt agcccatcag cagcaatccc
     2041 ttgccggttt cagcgctcgc atcgcccagg cgccagatca agttgcccat ggcatcgatt
     2101 gcgccggcgc ttccggtcaa ctccgccaaa cgcggcgcga ccatttgcgc gataaacttt
     2161 tttaactgag gatcgtcttc gagccgttcg gtctggggtg aggggtaacg caagagatcg
     2221 acaagcagcc ggtcgatctc ttcgtcactg gcaacttgat cgacggcagt gaatacttgc
     2281 gcggaagtca tcatgatcga cggctgcggc gcgccatcac caggcttgct ctaatacttc
     2341 gagaacttgc tcggcggcgg ttaccggttt gggattggtg tgaatactgt aatcatccaa
     2401 agcatcgctg gcgagctgtc tgagaccctc gcgcggcaca cccagctcac gcagtctgag
     2461 cggcagatcc atcctccggt tcagggtgaa gacgtgctca ggaacggcgg cagcgagcgc
     2521 gttttcatcg gcgccttcga tgccgatctc tcgcgccatc cgcgcatgcg cctctaccgt
     2581 agcgtcgaga ttccagcgca tggcgtacgg cagcatcaaa gcatggatct cggcgtgcgg
     2641 cgctttatag cggccgccga tcacgtgaac gatcgcatga tgaatgccgg tccaagcgct
     2701 gtaaaccccc atacccgaga gacaagctgc tacctgaacc tgactcaagg cttcgaggtc
     2761 ccgggaattt tccacggcgc gcggcagatc gcgcgcaatc agcccgatac ccctgagaca
     2821 gtaagcgtcg gtaagcggct gcgtttcagt tgagtacatc gcctcgacgc aatgcgctag
     2881 cgcgttcatc cccgaagctg cgagcaatct tgccggggcg gtcgccgccg cctccggatc
     2941 gagaagcacc acgcgcggcg caagtttagg atcggcgata atatacttct tgccgccttc
     3001 gctgatacct gccgagtaac tgtactctcc gccggaaaga gtcgtcggaa tcgcgatatg
     3061 cggcagcttc gcggccggca tcggcggaac ttctttctcg ttgggcgggg taaagcgcac
     3121 gctgagacgc ggcaacgagc cgccttcggc cagcagagcc gcgacaccct tggcggtatc
     3181 gatagcgctt ccgccaccgg cactgaccag cacatcgatg cgcgcccggc gcgcgctttc
     3241 gaccgctttg gcaatcgact ccgtcggcga atgcggtttg acctcatcga acaccgctac
     3301 gcatttagct cccaattcgg ccctgatccg atcgagcaag agcgttttgg caaccgacgg
     3361 cgagatcacg atcatggcgc gggttccctc gagcctgccg atctctccgg ataatttttc
     3421 aatcgatccc ggcccggaaa cgatgcgctc gacccgcgga ttgttaaaaa caaaagttgc
     3481 agccgccatt ttgtccgcct attttaaaat ttctctcagc cgggcgacca gaggcgggtc
     3541 aagacgaaga agctctttga cattgcgctc gagatcttcg cccgaccccg gattgatttc
     3601 caaattggct ttggccgcct ccttgagcaa ttccggatct ttgacagtct ccataaaagc
     3661 cgtgcgcagg atctccacgc gatccttagg cgtccctgga ggcaacacgt aaggacgcac
     3721 cgcggcgcca tgagcttggg ccactgcctg catcagtttt cgcgcctcgt ctgtcttggc
     3781 gatactcata gtcaaaggca ccttgggaag atccggatgg ggcttggcac taacctgaag
     3841 cacaatgatc acctcgccgt tatcaagctc tttcctccac gtcgaacgaa acgactgcca
     3901 cgaattgctc aggccgccga cctcgtcgct gttgaatgcc aagcgaatcg gcgcggtccc
     3961 tttataaccc gaaaccacct ggatcggcag gccgagagcg atttcggcaa ccttgggaat
     4021 atcataggtg ccgtcaccgg ctgctatcgc gccgaacttg accgctgtct tcgatgccaa
     4081 ccattgctcg atgtctgtga tgcccgtggt cttggagact ccaaaaacaa aattgtcctg
     4141 agccggtacc ccgacgtatt cgaatttgag cgcatcgaat tcaatgccgg gcttgccgag
     4201 gagctgttga agaaattgtc cgccaacgaa atggccgatg gtcaggccgt cgggtttggc
     4261 taccttgaag agatggttcg ccgagaccaa tccgcccgcg ccaggcatgt tatcaacgat
     4321 gatggcaggt ttgccgggaa tatgattgct gaaatggcgg gcaatcaggc gtgtgtaggt
     4381 atcgtacccg ccgcctggcg aggtcccgac gattatacga attgtttttc cttcgtagaa
     4441 cggcgccgcg ccatgagcgg gttggtcatt gacacccagc gcaatcagca gaacggcgca
     4501 agcggtcgtt aaaccccgga acctcactag caaacttttc aaaattttga ctggcatttg
     4561 tttcattgct cctttcgcaa tgttttttca agagcaaaac gagctgctac gacgtagccg
     4621 tgattgcaaa caccgcattg ccgaacaacc gacaccaacc caacttttcg aaagcacttg
     4681 tcgtacattc cgccgtctac gacgagccgc tcgtgctgtc aagggatacc ttcacatctc
     4741 acaagcaatc tcagttaggc gtcaaccaaa ggagcacctt caatggtgat ctcttgccag
     4801 ggtgatagct gatttgctac caatcacgaa acgtttagca ggagagtgac caacatgaaa
     4861 ctcgctgaac tgcactggcc ggaagttgaa aagctcgaca gagaaaaaat agtcgccttg
     4921 attccggtgg ggtccatgga gcaacatggt ccgcacctgc cctttcaggt cgatgttttt
     4981 tgcgcctcgc gcctggccga ggatctcgaa aagaaaattc cggacgtcct gctggtgcca
     5041 ccgctttggg cgggtgtttc ggcgcaccat atggatttcc cgggctctat cactttgcgc
     5101 gccaaagtct tcatcgacgt tttacatgac atctgcggtt cgctacatca tcacggcttc
     5161 cgccggatcg tgctattaaa tggccacggc ggcaatcgct ctagcctgga ggtgctggga
     5221 caagaactct tcgtcgagtt cggcctgacc gccaacacgt tggcttattg ggacttggtg
     5281 ccggatctag tcaagtcgct caagaaaacc caatcgatcg gcatgggtca ttcgggggaa
     5341 ctcgaaacat cgctgatgct ttatctcgcg cccgaattgg tcgatcggca agctatcccg
     5401 gcgggtgttc tcggtgtcga gccgcccggt cccacgagcg ggatcaaacg ttacgtcaat
     5461 atgaaggagc attcggcagc aggcgtgatc ggcatgccgt cggcggccgc tcccgagatc
     5521 ggcaaagagc tctatcaggg cgcgctaaac gcgctcgaga aggcggttcg cgtccttcag
     5581 tctgaccatt gattaaagcg cagcgcgttt cattttttga aaaactttgc atatttctgg
     5641 ccgagctgct tgtaatcgcc ttcgctgcgc tggatctctt cgacgaattt gattttgtcg
     5701 gcgtctttca acggcggatg aactccgcgc aaattgacga attcgcaatc cttggcgagc
     5761 gcttccatgc tatctcgact gtaccaatgg tcgatccata attttgccgc gttaacgttg
     5821 gccgacttgg agccgattgc gatgtagttg ctttctccca ggtacgccgg cagccgtacg
     5881 tagtccaaag gagcgccggc ttttcccata tcgcaaacat attttatgta tgcgatgccg
     5941 agcggcttct caccgttggc gatgctttcc acggccggtg tcagggagcg gtgcagcagc
     6001 ggccggctcg cggccaggcg ctgagcgtat tcgtcttctt tgcttctgtc gcccaacacc
     6061 aaatgcaaac tcgagagcca tttggccatg gaggtgtgcg aggtcggatc ggacatcgtg
     6121 gtcttcccgt cccatttcgg atcgacatag tcccagtagg atttaggcgc gtcggacggc
     6181 ttgatatagc gggtgttgta gacaaagccg ataatcgagc tgcgataatt ggggctatag
     6241 aaatcgtgat gatacgaagg atcgaagttc ctggccgccg gcgtttgata tttgagaaac
     6301 atcccctcgt ttctcatgat ctccatcgca tccaccccgg taaagaaaat atcccaacta
     6361 accttgccgg tgcggaattc attgatcgcc cgatccatga ccgcggtggt tgatgcacgc
     6421 cagtagacgc ttttgacgcc tggatatttt ttatcgaagc tcccctggat ttctttcatg
     6481 acctcgtctt ggagagagcc aaaaatagtg actttgccct cctttcgcgc ggcctcgatc
     6541 agcgctgttt cctgtccgtt agccgagcgg caaagaacca agaaaaacgg cgaaaacatg
     6601 gcacagacca ggatcagtct tcgatgaagt gtgcttttct tcataataat ctcctttgat
     6661 tgggtcaaat actacaacgc catatcgagt aaccggctga ctttttccgc tatagccgcg
     6721 cctgcaggaa agcgactatg atccggtgat cgttctgaat tgtccgccaa ctggttgaac
     6781 ttgcgacccc attccagctg cggcacgatg tggagttgct ccggcgcttc ctgcagccag
     6841 cgaacgtcct tgtgtactgt ccaataggcc tagcttctgc gcagaatctc ctgcgcttct
     6901 ttggaaagat aaaaatcggc cagcagaacc gccgcatgcg gattcgggag cctcgtgata
     6961 gtagccgtta agggatcaaa aaagagcagt ccgttttttt aatctcgatc ctattcaagt
     7021 ccatgcaacg accgccaatc tatactccac ttattacata tattgtccag aaccggattg
     7081 cccgaccgct ggcatattcg gtgatctatt gcgctcatcg cagcgcgctc tgatcacgat
     7141 cgacgttgcg ttgtggtttc gccatccagt caaagccgat gaaaaaacat aatgaccgag
     7201 ctcggcaagc ttatctgcag tagatgaaga acgtgccagg ctggattgaa agctggagag
     7261 gaaaacgaaa gtggaaaaga aagacgcggg ggcgagcgtg tcgcgcagaa ggcgacatca
     7321 agcgaaggag cgaataaaac ttctaaagcg gaatcgcctt ttgagaaacc ccagtgttcg
     7381 gatttgatac aatgatatta tgatcgcaga atcttagagc ctttggagcg tgaccgatgg
     7441 cacgttaaca gggtcagcac ctctgaacct aatcgatggt gtgttgagag gcggcttgag
     7501 ccgcctctca acagtgaacc tggaataatt ctaattgaga agcgcacaat tcaactacaa
     7561 atcactaacg accgaaacga tcattgttcc accagctgta gcggctgtca cggctattaa
     7621 accatccact ccggcgccga tcccaacgat cgcgatcgtt ccaagtacca tacattctct
     7681 ggttcccgcc cctataccaa tgattgtcat agccggactt gcgtagatta ctgcgcagtt
     7741 cagattggtc acgtctcagt tcgcgccggt ctcgctcgat ttctctcaca tcgcgcctga
     7801 tttcggattt gccccgggca atctcgtgtc gcggtgctcc ccggcgtatg tcgctgcgca
     7861 gctcggcgcg atcacgccta agttcagcga agtcctgccg caactccgcc cgattctttt
     7921 gaacatcacg gtgactctcc cggattgcgc tgcgcccttc ccgatgcgac cgtccgctat
     7981 cggccaaagc agaatcactg agcattaatg ctgaaagaaa gccaatggca agccagatca
     8041 aggttttccc attttgcatg attttccctc ccttgcgcct tagacgcagg tataaagcga
     8101 atgttaatcg ccgacgaaaa cgatccggca gatcatttga tacggatcta cgttcgctga
     8161 cgggacaact agacatgcct tatcagccgc cgagccaaga acgttgctcg ataagaacgt
     8221 cacgatgctt gcgtcctcct gacagcaaat attttcaagg aaggctcagc acgcgacggt
     8281 actgagcgat caaaacacta agcttcccac tcatttggcg tagaatgtcg cgctagctaa
     8341 tggcccgaaa gtttcaatcg atctccgaac ggatcggagg gtgaccctgc tgatagcagt
     8401 acatttaggc gcattcaaat cgcagcggga gcggagcacc aaccatgaat ccgatgcaga
     8461 tctaggcaag agctatgcgg gaaatcagga taaaagaaga gtaaaattca agtcgctgaa
     8521 tcggttctga cactcggcgg tgcaaacatt catgtcatta cggattgttt gcgttgcgcg
     8581 gtcgaaccgc gcattacatg acgagtaggt acgttaggct atggcagttt tgtagttatg
     8641 tccgttgcaa tcaacgccgc agatctcggc cactgcatct cgataggata accagcccgc
     8701 caatgcttct tgcatggctc tgacaaccgt gccacgcctc gtcggtttta cgtgatctag
     8761 aatcgcggcc catgcagcgg cccgatggtt tccctcaacc tgccggtgcg ctttggtgag
     8821 agctaaatgt tggatcggca ggccgtaatg cttgacgagc ggatgctcat ctaatggagg
     8881 tggcggaacg gctaccgtcg attcgatcgc gcgccgttcg tccttcgacc cttcgacaaa
     8941 gatggtgaca actgctgcgg cgatctccca accataatgc tgagtcgcgt tatcgaggta
     9001 ctgccgatag ttctttgcgg ccgggagcag cgatacattg gcgaagcgct ttaagtccat
     9061 tcctaggccg cgtgcatatt caagaaacag ctcagggtgc gggctgccgg cgaccagccc
     9121 accggtctct tcttcgtaaa gattttctgc gagactgcgc cgaactaggg caatcgggca
     9181 gcgcacgtaa gcgcgcccga ccatcacggg aaaatcgcgg acataagtcg catattcctg
     9241 ttcgaggtga tgatggagaa gatctttcgg aacctgccct gaggtaaatg ccggccaagc
     9301 ccaatggtcc ttgccttcca tcacaactag gagctgttcg cgaaaagttg ctcgatccat
     9361 gttgctcccc cttgcgaata atcttcgcaa gggtggcgac agcaactacc atgccgccca
     9421 agccggatcc gtagttgtat ctattcgcgg atttcgcagt ccaatagcat ggttgtcgat
     9481 gtttgtccga taatctctac ggggcgaatg ggtcgaaaat tcaaccgaga gttgagccgg
     9541 gcacggacga gaaatgcgtc agaatcgaga tgctcgcggg cagatacgcc actgaagacc
     9601 gattcggtat tcaacgctat cgtgttttgg ttcgcattgt gacgaactct tccgcggctg
     9661 tcggatggat tccgatagtc gtgtcgaact gtgtcttggt cgcaccacag ttgagcgcta
     9721 cggcaaatcc ctgaataatc tcgccggcat tggctccgac catatggcag ccaagcaccc
     9781 ggtcgctcac atgatcgacg acgagtttca tcatcgtctt ttccgagctt ccggtcatgg
     9841 tatgccgcaa tggtctgaac gtgcccatgt acacatcgac ctcgaattca cgacggcgcg
     9901 cctgctcttc ggttaaaccg actgtgccgc aattaggcgt gctgaaaatc gcagtcgcga
     9961 cattttcata atccagcata atcggtctgt tgttgaacag cgtattcgcc acggccgtac
    10021 cctcggcgat ggcaactgga gtgagcatca agcggtccgt gcaatcaccg atggcgtaga
    10081 tactctcaac gctcgagcgc aagtacggat ccacgactac cgcgccgctc tgtctcaact
    10141 cgacaccggc gttttccaga cccaaacggc gagtcttagg cacgcggccg gtggcataga
    10201 gaatctgatt ggcgtcgatt acgctgccat cgatgagggt ggcgcgcaat atttcgccat
    10261 gcttttcgat gttggcgata ccggtgttaa aacgtagatc gatccctcgc ttgagcatct
    10321 catccgcgag ggtaaggcga atatcattgt cgaagccgcg cagaaacaac tcgccgcgat
    10381 acagctgcgc cgtctttgcg cccagcccat ggaagattcc tgcgaactcg actccgatgt
    10441 acccgccgcc gacgacaact actttatccg gcagctcggg aaggtaaaaa gcttcgttag
    10501 aggtgattac gtgttgggat ccgggaaata tcgggataga cggccaactg cccacggcga
    10561 tgaggatata ctttgtggtg tatcgcttgc cgtcgaccgc gaccgtatgc gggtctacga
    10621 cttgagcgtg accttcgatt atctgtacac cggcatcatc gagcagttta cggtaaacac
    10681 cattgagccg gtaaatctcg ctatccttgt tggcaatcag tttcgcccaa tcgaggctct
    10741 gcggccccgt tgtccagccg aaaccggcag cgtcgctaaa gtcgtccgaa taatgagagg
    10801 cgtaaaccag aagctttttc gggatacagc cgacattcac gcaggtgccg ccgagagcgc
    10861 tttcttcggc aacggccacc ttcgcgccaa acgatgcggc aacgcgactg ccccggacac
    10921 cacctgagcc ggctccgatg gtaaacaaat catagtcgta ttgagccatg aaaaccgcct
    10981 ttcaaggatc tagttatgat aaaaggtcca ttcccattcc tcaacagtaa gactgttcac
    11041 cggaatcggc tttgacactg tacaggattt gaacgagaat gccgtcactt gctagatcct
    11101 tgtttttgcc gacgatgcca ccctctttat tccggagcac ctctaccgga agccgtcgtt
    11161 tactcgcggc tgtgagcgct ctgcgccatc gcaactatcg tctctactgg ttcgggcagc
    11221 tcttttccgt gctggcgcaa aacatggaag ctgtcgccca gagttggctc gtgcttgaaa
    11281 tcaccaactc gccgctacta ctcggtctaa ccggattaac cttctcgatt cctaccatcg
    11341 cgctgacact tctcggcgga gcggtggccg atcgcaccga ccgcaagcgg attatgattt
    11401 gctcgcagat cggatcgttt gtgatgtttt tattattggc gattctcgtc gtaaatgaac
    11461 tggtggtgct ttggcacgtc atgactgcgg ccttcttctc aggttgtttc agggctttcg
    11521 accggccgag ccggatggct ctcctgccgc tgatggtgcc gaagcaagat attccgaatg
    11581 ccgtcgcaat cggcggcacc atctggcaat taaacaagct ggtcggcccg gcgctggcgg
    11641 gaatgctgat atatttggtc agtatcggcg cgacttatct gttctgctgt gccgcttcag
    11701 gaaccgccat cttactctgg ctcggcatcc gcctggatcg ccagcggatc accgccactg
    11761 cgggtggcct tctgcaaaat atgctggacg ggttaaattt cattcgcaaa aatgaaatct
    11821 attacagctt catcggcatg acgtttttca acagcgtttt cggcatgtct tacttgattt
    11881 tgatgccggt gtttgcgcgc gatatcctgt tcgtcggctc cgaaggcttc ggtctcctgc
    11941 agagcgcggg cggggccggc gcgctttgcg gagttttact agtcgcctat ttcggtcagg
    12001 cgcgcggcaa gggtaaacag gcactcagcg gcgccctggt cttcggcatg ctgctgattg
    12061 tctttgcata ttccaattcg tataggctgt cgctcgcaat ggcgtgcgtg ctggggatgg
    12121 cgagccaatt ctacatgacg acgattcatg caattctgca agtcaaccta cccgaccaat
    12181 tacgcgggcg cgtgatgggg attcatggat tggcctggga gctaatgcct gtgggcggca
    12241 tgatcggcgg ggtcatcgct gaggttaacg gcgcgccatc ggcggtggca tttggcggcg
    12301 ctatggttgc gggaatggca ctggttgtgg ccgtattcct gccgagcatc cggcggttgg
    12361 aacagtaatg ttagttgtgt taagcaagcc gaacacactg caatggcgga gaagaaggcg
    12421 aaaaagaaat ccggcacagc ccgtaagcgt cgcaagctcg ccgggaaatc caccggatta
    12481 acgccgacgg aacttgtcgg catagctccc gccgttatcg aaagtctgga acgcgccgtt
    12541 gaggaagacg gcggcagagt gatcagcagt tatcgcgaac cgtttggcgg ccaatggttg
    12601 atcatggctt cgttgcccat ccagcaagtc gaaccaacgc cctatcaacg caatctttcc
    12661 gatactcacg tgcgcaagct cgaaaccgtc attgcgaaac tcggccgctt cctcgatccg
    12721 atcatagtcg tgcgcggcga accgaatcaa gacggagttc gctattggac tcccaacggc
    12781 aatcatcggc tatcggcgct gcgcactttg ggcgccaagt cggtcactgc gatagtagtg
    12841 ccggaaactt caaccgctta caaaattctc gccttgaaca cggaaaaaag tcacaacctc
    12901 cgagaacgag ccatggaagt gataaagatg tatcaggagc tggctatgct cgatggagcg
    12961 gacgaagaaa gctatgcgct cgagtttgaa gacgcggcgc tcatcacctt gggattgtgt
    13021 tatcttgaac aaggccgctt tagcggcggc gcataccacc cgattcttcg gcgcgttgat
    13081 ggtttcctaa ggaagccttt gcaagcagca ttggaaatcc gccgcgaacg ttccaagatg
    13141 ttgatggatt tggacaagaa ggtttgtgaa agagtcgata tgcttaagaa gcgaggtcta
    13201 accagtccct accttaagag cttcgttgtg gcgcgcatca atccattccg cttccggcca
    13261 aaagacgtgg agccgctcag ctataacgaa gtgctggaac gaatgatcaa agctgtcgat
    13321 aaattcagcc cggaaaagat caggatggaa gatttggcga agtcaggcgg cgcgcctgag
    13381 gaataacgtg cggattgcgg cattcagaac ttttgctagt ggcgacctca ttcccggtga
    13441 atcttgcgaa aacggcagcg gcgacttctc tgctttcctc gatgtttcgc tg
//
LOCUS       DASUVS010000005         3969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1311958, whole genome shotgun sequence.
ACCESSION   DASUVS010000005 DASUVS010000000
VERSION     DASUVS010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311958"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VF182_00150"
     CDS             <1..504
                     /locus_tag="VF182_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435511.1"
                     /translation="VGDGGPAARVAALSSGVIHATMFTPPSDKISEKAGMSILTRLDV
                     ANVGAGINTTPGFIQKNRPQALAFLRGYMEGIHYLKANKDESLKIFSKYVRNPDLGIM
                     AYLYEEISTRAEKDLRPQGEAVRALLDLAALDFPHAKKLSEKDNWDLSLIDEIQRSGF
                     VDQLYRK"
     gene            623..2344
                     /locus_tag="VF182_00155"
     CDS             623..2344
                     /locus_tag="VF182_00155"
                     /inference="COORDINATES: protein motif:HMM:NF006203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX6435512.1"
                     /translation="MEFNKTVTLPVETVGQAYLEVLRARGIKYFFGNSGTDFGPIIDA
                     LAKLAADKKEFPSPITVAHEFVAVSMAQGYAMITGEPQVVMVHVIVGTGNASAAVMNA
                     SRLNVPMIFSAGRTPLTEEGLTGSRSNFIHWGQESFDQGSLLREFTRWDYELRNGQQV
                     ETVVDRALEMTLGTPQGPVYLTLPREVLAQRMDSITIHPYRKSREEPLQPSAESIRRT
                     AEILRGATNPLIITGRLGSDPTAVSAMVSFAEALVAPVVAPASPFVSMPNTHDYHLGV
                     SSAPYIKDADVVVVVESDVPWYPAQGKPPETAKVIQIARDPNYIGVPIRSFPKDISIG
                     GDPKLALQLLAEELRKGPRNENALSERAERIRARHKKYREDVKQKAEKASGHRPISHA
                     WLAYCVDQVRDRDTIVLSDHGIGNEVLNLTEPETYFGGSPAGGLGWGIGGGLGMKLAR
                     PEKTFIVTVGDGTYMFNNPTACHLVSQSYGLPILTIICNNAIWHASKAATQQVMPDGW
                     AVSTGNFPLCDLTPSPRYEKIVEAHDGYGEMVDDPQQVLPALQRALKIVKEEKRQAVL
                     NVICGSH"
     gene            complement(2456..2629)
                     /locus_tag="VF182_00160"
     CDS             complement(2456..2629)
                     /locus_tag="VF182_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435513.1"
                     /translation="MRNRDWLHHKRRALYQIGLFVLERVVRNAGRGDQKAEGAGDSDI
                     GATYYLIGLRPST"
     gene            2689..3846
                     /locus_tag="VF182_00165"
     CDS             2689..3846
                     /locus_tag="VF182_00165"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6435514.1"
                     /translation="MLVIDSDGHLHEPFYLFDRYMEKEFFSMRPRVIGLLDDPREEGR
                     WFAEGKIVPRLPFTKGVGGGGFKYQTPRHKEMKAKDLTLDDIEGRLQDMEQLGVDYQI
                     AYPTALAWVFDLENKELAAAVCRAYNNYVAEQCSKASKRLGGVALVPIQDPPAAVAEA
                     TRAVKKLGLVAVTIPGLVGNKPIHSPEFLPFFRAMNDLDVPIGFHAFTGMNYTPWADC
                     FDDFFSTHITAMPFSMMVAMMSVARMGLLKNLPNLRCAFLEVGASWILYWAWWVGRHI
                     EHTVGLRADGRNEAGVWGRQPYELPETVHEPLEDIREGRMTSGFEVDEDLRLLIDKLG
                     TKTLMYASDYPHGDMEWGRVQAVQANQDLTQEEKAALLGGNAMRFHKLNFE"
BASE COUNT          919 a         1058 c         1158 g          834 t
ORIGIN      
        1 gtcggcgacg gcggcccggc ggcgcgcgta gcggccctgt cgagtggcgt gattcacgcg
       61 accatgttca cgccgccatc ggacaagatc tccgagaagg cgggcatgag cattcttacc
      121 cggctggatg tcgccaacgt cggcgccggc atcaatacga cgccgggatt tattcagaaa
      181 aaccggcctc aagcgctggc ctttctgcgc ggttatatgg aagggattca ctacttgaag
      241 gcgaacaaag acgagagcct gaaaattttt tccaagtatg tgcgcaaccc cgacctcggt
      301 atcatggctt atctctacga agagatttcc acgcgggcgg aaaaagatct gcggccgcaa
      361 ggcgaagccg tccgcgcgct gctcgatctc gccgccctcg attttcccca tgcaaagaaa
      421 ctatccgaga aagacaactg ggatctgagc ctcatcgacg aaattcagcg ctcgggcttt
      481 gtcgatcagc tgtatcggaa gtaaatggga agctaacagg agtgaagtgt aaataataag
      541 gaatcaagcc gggccaaatc cgtgtccgct tcctggtcct aattccgttc actcgtcaaa
      601 atgacaaaag cgaggagtcc ccatggaatt taacaagacc gtaactctac ccgtcgaaac
      661 cgtcggccag gcctatttag aagttctgcg cgcgcggggt atcaaatatt ttttcggcaa
      721 cagcggaact gacttcggcc cgatcatcga tgcgttggcc aaactggcgg cggacaagaa
      781 agaatttccc agtccgatta cggttgccca cgagttcgtc gccgtgagca tggcgcaggg
      841 ctatgcgatg atcaccggtg agccgcaggt cgtcatggtt cacgtgatcg tcggcacggg
      901 aaacgcttcg gccgcggtga tgaatgcgtc gcggctgaat gtgccgatga ttttttccgc
      961 gggacggaca ccgctcaccg aagaagggct gaccggcagc cggagcaatt tcatccactg
     1021 gggccaggaa tccttcgatc agggcagttt gctgcgcgag ttcacccgct gggattacga
     1081 gctgcgcaac ggtcagcaag tggaaacagt ggtcgatcgc gcgctagaaa tgacgctggg
     1141 cacgccgcaa gggccggtgt acttgaccct gccgcgcgaa gtcctggcgc agaggatgga
     1201 ctcgatcacc atccatccgt atcggaaaag cagagaagag ccgttgcagc cgagcgcaga
     1261 atcgatcagg cgtaccgcag agatcctgcg cggtgcgacc aatccgttga tcatcaccgg
     1321 gcggctgggc tccgatccga cggcggtgag cgcgatggtt tcctttgcgg aggcgttggt
     1381 tgcgcccgta gtcgcgccgg caagtccgtt cgtgagcatg cccaacacgc acgactatca
     1441 ccttggcgtg agttcggcgc cttatatcaa agacgccgat gtggtcgtgg tcgtcgaaag
     1501 cgatgtgccc tggtatccag ctcagggaaa gccgccggag acggccaagg tcatccagat
     1561 cgcgcgcgac ccgaactaca tcggcgtgcc gatccgcagc tttccgaaag atatttccat
     1621 cggcggcgat cccaagctgg cgctccaatt gctggcagaa gaattacgaa aaggaccgcg
     1681 taatgagaat gcgctttcgg agcgcgccga gcgcattcgc gccaggcata agaaatatcg
     1741 tgaggacgtg aagcaaaaag ccgagaaggc gagcgggcac cggccgatca gccacgcctg
     1801 gctggcgtac tgcgtcgatc aggtgcgcga ccgcgatacc atcgtgttga gcgatcatgg
     1861 catcggcaac gaagtgctca acttgactga accggaaacc tatttcggcg gctcgcccgc
     1921 cggaggattg ggttggggca tcggcggcgg tttgggcatg aagctcgcgc gcccggaaaa
     1981 aactttcatc gttaccgtcg gtgatggaac ctacatgttc aacaatccga ccgcctgcca
     2041 cctagtgtcg caatcttacg gtctgccgat cctgacgatc atctgtaaca atgcgatctg
     2101 gcacgccagc aaagcggcga cacagcaggt gatgccggac ggctgggcgg tcagcacggg
     2161 caatttcccg ctgtgcgatc tcacgccatc gccgcgttat gaaaaaatcg tcgaagcgca
     2221 cgacggttac ggcgagatgg tcgacgatcc gcagcaagtc ctaccggcgc tgcagcgggc
     2281 gctcaaaatc gtcaaagagg agaaacgcca ggcggtgctg aacgtgattt gcggcagtca
     2341 ctgacggtgg cgagtttatt cccggtcggc cgttgaaacc ggtccggcgc cttcgtgtcg
     2401 aggtcgcgcc agtgcggcga tcgatgtcct caccgcgcag caccgatcat gcgtttcatg
     2461 tcgaaggtcg caagccaata agatagtagg ttgcgccgat gtccgaatcg ccggcgccct
     2521 ccgctttttg atctcctcgc ccggcattgc ggaccactcg ctcgaggacg aacagaccta
     2581 tttgatagag cgcccgtcgc ttatgatgca accaatctcg atttcgcatt gacacgcctg
     2641 taggcacttt gttaatcgca gggaatcttg gacaaggcag gttaaaaaat gctggttatt
     2701 gattcagacg gtcatttgca cgagcccttc tatctcttcg accggtacat ggaaaaggaa
     2761 tttttctcta tgcgaccgcg ggttatcggt ctcctcgatg atccacggga ggaagggcgg
     2821 tggtttgcag aagggaaaat tgttccccgc ctacctttta caaaaggggt tggcggcggc
     2881 ggcttcaaat atcagacacc ccggcataag gaaatgaaag ccaaagacct tacgctcgat
     2941 gacatagagg gcagacttca ggacatggag caattaggag tcgattatca aatcgcctat
     3001 cccacggctt tagcatgggt gtttgatctt gagaacaagg aactggctgc tgctgtttgt
     3061 cgagcctaca acaattacgt tgcggagcag tgctctaaag cttcaaaaag gctcggtggc
     3121 gttgcgttag taccgattca ggatcctcca gcagcagtcg cggaagcaac aagagcagtc
     3181 aaaaaattag ggctcgtcgc cgttaccata ccagggttgg ttgggaacaa gccgatacac
     3241 tcgccggaat ttctaccgtt cttccgggcg atgaacgatt tggatgtacc aattggcttc
     3301 catgcgttca ccgggatgaa ctacactcct tgggcagact gtttcgatga cttcttttcg
     3361 actcatatca ccgcgatgcc tttttcgatg atggtggcaa tgatgtcagt ggcgcgaatg
     3421 ggactgctta agaatcttcc aaatcttcgg tgcgcttttc tcgaggtagg agcaagttgg
     3481 attctatatt gggcctggtg ggtcggcagg catatcgagc atacggtggg actacgcgcc
     3541 gatggtcgca atgaggccgg ggtgtgggga cgccaaccgt acgaattgcc cgagactgta
     3601 cacgaacctt tagaagacat ccgcgaggga cggatgacaa gcggttttga agtggatgag
     3661 gatctgcgcc tgctaatcga caagttagga accaagactc ttatgtatgc aagcgactat
     3721 ccacatggcg acatggagtg gggccgagtg caagcagtac aagccaacca ggacttaact
     3781 caggaggaga aagctgcgct gctcggcggc aatgcaatgc gttttcacaa actgaacttt
     3841 gaatagatca tacttcaaat cgcctgtgtc cggagatcca caatgctcaa cgtgaagcat
     3901 gtttttttac cgttttttgt tctggctctc gcgctatcgt cctatgctca agaaacaaag
     3961 ctagttgat
//
LOCUS       DASUVS010000006        11471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1093540, whole genome shotgun sequence.
ACCESSION   DASUVS010000006 DASUVS010000000
VERSION     DASUVS010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11471
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093540"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4725)
                     /locus_tag="VF182_00170"
     CDS             complement(<1..4725)
                     /locus_tag="VF182_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX6435515.1"
                     /translation="MKLPFRQKLLRKKHSEAIALSQLEEPIRAELFSSERLEQHGESL
                     AVAQRVTMTPRKGRPLLPRVVDNGRVLLESYRTIAQAVREERAITPAAEWLVDNYHII
                     DEQLREIHDDLPSGYYRELPKLAEGHLQGYPRVFGLAWAFIAHTDSRFDPEMLRRFVR
                     AYQRVQPLTIGELWAVAITLRVVLLENLRRLAERMVHERAARQEADAVADRLLGVGGS
                     SIERAATVLRPFEGASLSTAFAVQLVQRLRDQDPVIMPALRWLEQRLAAQGTTADEIV
                     RVEHQRQAAMNVTVRNVITSMRLISAFDWAEFFESVSLVDAILWIDTDFAAPDFVTRD
                     RYRRAIEELSRGSPHDEREVARLAVFQAKRSRGTAQEVDTATDRRADPGYYLIANGRV
                     AFEQKLGFRMPIKTWLRRAYLTNATSRYLATIAIVSGLILTLFLFYSGAAEVGVVSLL
                     IVGLLALVPASDLAIAFVNREVTELLGPRVLPRLALRDGVPADLRTLVVVPTFLIDQV
                     QIAEQIERLEVHYLANSEGDLRFALVSDWTDAPHENMPGDDELLAAAVDGIAQLNRRH
                     GFAADGSERFFLFHRWRRWNESERNWIGWERKRGKLHELNRLLRGATDTTFVPTGGRA
                     PIAPAAVRYVITLDADTRLPRGTACRLIGTMAHPLNRPRFDPGVGRVVEGYALLQPRV
                     TPTLPTDREGTLFQRISSGPAGIDPYASAVSDVYQDLFGEGSYIGKGIYDVDAFEAAL
                     VGRVPENALLSHDLFEGIFARAGLVTDIEFFEEFPSHYEVAAVRQHRWARGDWQLLPW
                     IMGRAPDPSGAPIPLIGRWKMLDNLRRTLFAPAAFLTLIAGWTLPFAVPWIWTTFVLA
                     CIAIPSLLQVLIGAIPRRRGISKRSHARAVGTDLVLAAAHVALTVTLLAHQAWLMTDA
                     IVRTVTRVYVTRRKLLEWVTAAQAKSGINLSLSSCYRRMNSTVALAVAVGILVVLFSP
                     EVWPIAAPFVFLWALAPLVAYWTSLPPRAAAAQPLSEDDVHTLRLTARRTWRFFETFV
                     GPEDHALPPDNFQEEPIPVVAHRTSPTNLGLYLLSILAAHDFGWIGTVAAIERLEATL
                     GTMNRLERFRGHFYNWYDTRDLRPLDPKYISSVDSGNLAGHLIALGQGCQEIGEHPLL
                     GPQTLSGIADALLILRQLARGIVDGRRTQTVTRKQLDEALDALTAALSPVPSTPGDWV
                     ARFTELGVRARTVADIARTLTAERGDSAEGELGAWAEGVRAAIESHARDLDAAMPWAR
                     LVYSKAPLNRTMVAEEDLVSTTMARFFLSVPTLADAPDRCESAKRELTMLRERVAKDR
                     AVQSDTLARIDAIIESLGRSAEACGALAGRLSALIQRTNTIFEEMKFGFLFDGTRKLF
                     SIGYRVSDNSLDPNCYDLLASEARLTSFIAIAKGDVPSAHWFHLGRALTPVDRGSALV
                     SWSGSMFEYLMPMLVMHSPSGSLIGQTCFLVVRRQRKYGSERGVPWGVSESAYNARDL
                     ELTYQYSNFGVPGLGLKRGLSEDVVVAPYATALAAMVDPKTAAENFLRLTEAGASGRY
                     GFYEALDYTVTRVPENQAVAVVH"
     gene            5513..5659
                     /locus_tag="VF182_00175"
     CDS             5513..5659
                     /locus_tag="VF182_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435516.1"
                     /translation="MPPLTEVEQNLQAHMIEKQRLAESIYCYVFTERSSRIPTYPPAF
                     APRP"
     gene            6114..6611
                     /locus_tag="VF182_00180"
     CDS             6114..6611
                     /locus_tag="VF182_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX6435517.1"
                     /translation="MAEKNKEKEMKAVSPWRPFRDLTSWDRDMERIMEDFFGRRMRPW
                     WPERWSRTEEMEVKPPIVDVFEEKDDIVVKAELPGIEKDNIEVNLTDHTLTIKGEKKK
                     EEEVKEEDYYRAERSYGSFIRSLDLPRDVRPDQVKASFKNGILEVRMPKTEEAKAKEI
                     KVKVE"
     gene            6669..7571
                     /locus_tag="VF182_00185"
     CDS             6669..7571
                     /locus_tag="VF182_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6435518.1"
                     /translation="MYSKILVPLDGSKTAENVLPLARSFARALQIPVELLGVIDIVEM
                     AYHVSTDRVPMIRSMIDDASRRSAEYLEGIAQNFPTGTTRWTVQTGSAAEAIINSAAT
                     NKQTLIALATHGRSGLNRWLLGSVAEKILRGGSNPTLVVRARHDKNPSWAMATLKRII
                     VPLDGSEMSEGILPYVKELAKQLDLEIKLIRLYGGLSAAGDGFYNPHQLDALTGRLRE
                     ETVAYLESKTDEMKQQGLNRISFAAKEGLEPDEILAIARETPDTMIAMCTHGRSGVKR
                     WALGSVTERVVRHSDNPVLVVRGS"
     gene            7601..8464
                     /locus_tag="VF182_00190"
     CDS             7601..8464
                     /locus_tag="VF182_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6435519.1"
                     /translation="MYSKILVPLDGSRTSEPILLYARLFADAYGIPVELLRVNDPDAR
                     PPFWPSESSGRYLKKIADEYFSGRASTSAEAGAPAAFIVDHAKTDPSCLIAMATHGLS
                     GARRWLLGSVTSKVVQTATNALLLIRPSHNFDASRPVRMDQIFVPLDGSALAEKALPH
                     ACALAKRLNPEIQLLRVYSLPMDAYVVADGVIARGPAQYREELAKQAQDYLEGKVAAL
                     RADGLERVVGIALEGDAASEIIDLASKTPDSLIAMSTHGRSGIGRWVLGSVAEKILQH
                     AHNPLLLIRAN"
     gene            8602..9027
                     /locus_tag="VF182_00195"
     CDS             8602..9027
                     /locus_tag="VF182_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6435520.1"
                     /translation="MLKTILAPTDFSEVSASGVRYACQLAKDVGAEIIIFNVDVLDES
                     NRVDKREIEQHKRRLAEFVTEKVPDAAVGLNMRQLVDAGQPFGAIVDCAEKEAVDLIV
                     MSSHGRSGLSRMLIGSVTDKILRGAPCPVLVVPLEQKKS"
     gene            complement(9077..9553)
                     /locus_tag="VF182_00200"
     CDS             complement(9077..9553)
                     /locus_tag="VF182_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6435521.1"
                     /translation="MKKPKAGTVSEIMMGSPVTLAPNDTLDLANDVISLGRIRHIPVL
                     ENGKLVGLISERDLMGAASDRIFGLKRATRSALLKTQLIKNIMKKRVSTVTPDTPVEV
                     AARVMAEKKIGCLPVLSNGALVGLVTTTNVLRYFGSKVTRKRHPSPPFRQARSAAK"
     gene            complement(9733..9999)
                     /locus_tag="VF182_00205"
     CDS             complement(9733..9999)
                     /locus_tag="VF182_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435522.1"
                     /translation="MDGFLAPKETLTRKRVEEILHEQQYSYFEQKVTLLEFRDDSLLL
                     KISFLMKYKNRMIVIEEKEPIVLSFSDFKVEDWGDGIKKIADVN"
     gene            complement(10071..10502)
                     /locus_tag="VF182_00210"
     CDS             complement(10071..10502)
                     /locus_tag="VF182_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6435523.1"
                     /translation="MKFNAINVNDVMSKDPLTVSASDTVGQAEELMYENHYRQLPVVE
                     NKQLIGIVTDRDIRSFLAQSSLLEPEKRGKALRTKVTDIMTAKPLTLSPENDLREAVE
                     LLLEEKIGGVPVVDETEGLVGIVTYVDVLRCFLDRLEDEQA"
     gene            10778..11242
                     /locus_tag="VF182_00215"
     CDS             10778..11242
                     /locus_tag="VF182_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6435524.1"
                     /translation="MDPIKKILAPTDLSDLSSAGLRYALEMGRSRGAEVIVYHVIDLG
                     DKWADRREESGPVREMLERHRRMLERFLWKNFPEEMNLTEVRQVVEFGGAVENIIEKA
                     EGERVDRIVMSTHGRTGIDHLLIGSVSEKVIARAPCPVLIVPAPRRNLARAA"
     gene            complement(11252..>11471)
                     /locus_tag="VF182_00220"
     CDS             complement(11252..>11471)
                     /locus_tag="VF182_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEX6435525.1"
                     /translation="AFVAAGFEHSVANMYFIPLGILLRENSDAANAELLTWGGLIANL
                     VPVTLGNIFGGGVMVALVYYFIYRHHAQ"
BASE COUNT         2599 a         3206 c         3286 g         2380 t
ORIGIN      
        1 gtgtacgacg gccactgctt ggttctcggg aactcgcgta accgtgtaat cgagcgcctc
       61 gtaaaagcca tagcggccgc tcgcgccggc ctcggtaaga cgcaggaaat tctctgccgc
      121 cgttttcggg tcaaccatcg ccgccagcgc cgttgcgtaa ggcgcgacga ccacatcttc
      181 actcaggccg cgtttgaggc ctaggcccgg tacgccaaaa ttcgagtact ggtaggtcag
      241 ctccagatcg cgtgcattgt acgcggattc cgaaaccccc cacggaacgc cacgctcgct
      301 gccatatttc ctctggcggc gcacgacgag gaaacaggtc tgacctatga gactgcccga
      361 cggcgagtgc atcacgagca tcggcatgag gtactcgaac attgacccgg accacgaaac
      421 cagcgcagac ccccgatcca ccggtgtcaa agcgcgaccg agatggaacc aatgggccga
      481 aggtacgtca cccttcgcga tcgcgatgaa gctagtaagt cgcgcctcag aggccagcag
      541 atcgtaacaa ttcggatcga ggctattgtc ggaaacgcga tagccgatcg agaagagttt
      601 acgcgtgcca tcgaaaagaa agccgaattt catctcctcg aatatcgtgt tcgtgcgctg
      661 gatgagagcc gaaaggcgcc cggccagcgc tccacaggct tcggcagaac gtccgagact
      721 ctctatgatc gcatcgattc gggcgagggt atcactctgt acggcgcgat ctttcgccac
      781 gcgttcgcgc agcatcgtga gttcgcgttt ggcgctttcg cagcggtccg gcgcatcggc
      841 taaagtcggg actgagagaa aaaagcgcgc cattgtcgta gaaaccaaat cttcttcagc
      901 gaccatcgtg cgattcaaag gagccttgct atagacaagc cgcgcccaag gcattgccgc
      961 atcgagatcg cgtgcatgac tttcgatggc cgctcgcact ccctccgccc atgcgcccaa
     1021 ctccccctcc gcgctgtcac ctcgttccgc ggtcaaggtg cgggcaatgt ccgccaccgt
     1081 gcgagcgcgg acgccgagct cagtaaagcg cgcgacccaa tcacctggag tgctaggaac
     1141 cggactgagc gcagcggtca aagcgtcgag tgcttcgtcc aactgtttcc gggtgaccgt
     1201 ttgggtgcgc cggccgtcaa cgatcccgcg cgccaattgg cggaggatga gcagggcatc
     1261 ggcgatcccc gagagcgtct gcgggccaag cagcgggtgc tcgccgatct cctggcagcc
     1321 ctgtcccagc gcgatgaggt ggccggcgag gtttccacta tcgaccgagg atatgtattt
     1381 aggatctagc ggacgaaggt cacgcgtatc ataccagtta taaaagtggc cccgaaaacg
     1441 ctcgaggcgg ttcatggtac cgagggtcgc ttcgagccgc tcgattgcgg ccaccgtacc
     1501 gatccacccg aaatcgtgcg cggcgaggat ggagagcaga tacagcccga ggttggtggg
     1561 cgacgtgcga tgggccacga ccggaatcgg ctcttcctga aagttgtcgg gcggtagcgc
     1621 gtggtcttcc gggccgacga aggtctcgaa gaagcgccag gtccgccggg cggttaagcg
     1681 cagcgtatga acgtcatcct ccgagagagg ttgagccgcc gccgcgcgcg gcggcaggct
     1741 agtccaataa gccaccaggg gcgctaacgc ccagagaaaa acgaacggtg ccgcaatcgg
     1801 ccacacttcc ggtgaaaaga gcacaacaag gatgcctacg gcgaccgcga gcgcaaccgt
     1861 gctgttcatg cgccgatagc agctgctgag gctgaggttg atcccggatt tggcttgcgc
     1921 tgccgtcacc cactcgagaa gctttcggcg tgtcacatag acccgcgtca cagtccggac
     1981 gatcgcgtcg gtcatcagcc aggcctggtg ggcaagtaat gtcaccgtca gtgcgacatg
     2041 cgcggcggca agtaccaaat ccgtgccgac cgctcgcgca tggctgcgtt ttgaaatgcc
     2101 gcggcgacgc gggatcgcgc caatcaatac ctgcaaaagc gaagggatag cgatacatgc
     2161 gagtacgaat gtcgtccaga tccaaggcac agcgaacggc agcgtccaac cagcaatcaa
     2221 agtcagaaaa gccgccggag cgaaaagtgt gcgccggaga ttatcaagca ttttccaccg
     2281 gccgatcagc ggaatcggag caccgctcgg gtccggtgcg cgtcccataa tccatggcag
     2341 caactgccag tcaccccgtg cccagcgatg ctgacgcacc gccgcaacct cgtagtggga
     2401 gggaaactcc tcgaagaact cgatatcggt gacgagtccc gcgcgcgcaa agatgccttc
     2461 aaagaggtca tgacttagta aagcgttctc cggcactctg ccgacgaggg cggcctcaaa
     2521 tgcgtcgaca tcataaatcc ctttgcctat gtaggaacct tcgccgaaaa gatcctgata
     2581 gacgtccgag acggcagagg cgtaagggtc gatgccagcg gggcccgagg agatcctctg
     2641 aaagagagta ccctcgcgat cggtcggaag agtcggtgtg acgcgcggtt gaagcaaggc
     2701 gtagccctca acgactcggc cgacaccggg gtcaaaccgg ggccggttga ggggatgcgc
     2761 cattgtgccg atcaatcgac aagcggtccc ccttggcaac cgagtgtcgg cgtctagagt
     2821 aatgacgtag cggacagcag caggcgctat aggcgctcta ccacccgttg gcacgaaggt
     2881 ggtgtcagtc gcgccgcgca gcaaccgatt cagttcatga agctttccac gtttacgttc
     2941 ccagccaatc caattgcgct cgctctcatt ccaacggcgc cagcggtgga aaaggaaaaa
     3001 tcgttcgctt ccatccgcgg cgaacccatg acggcggttc agttgcgcga taccgtcgac
     3061 ggccgcggcc aacagttcgt cgtctcccgg catattttcg tgcggcgcgt ctgtccagtc
     3121 ggagacgagg gcaaaacgaa gatcgccctc ggaattcgcc aggtagtgca cctccagccg
     3181 ctcaatctgc tccgcaatct gaacttggtc tatcaggaag gttgggacga caaccagggt
     3241 gcgtagatcc gctggcactc catcacggag cgcaagccta ggcagcaccc ttggcccgag
     3301 caactccgta acctcgcgat tgacgaatgc gatcgccaga tccgaagccg gcacgagagc
     3361 gaggagtccg acgattaaca gactgacaac cccaacttcc gctgcgccgg aatagaacag
     3421 gaacaaagtc aggatgaggc cgctgacaat cgcgatggtt gcaagatacc gggacgtcgc
     3481 gttcgtaaga taggcgcggc gcagccacgt cttgatcggc atgcgaaagc cgagtttctg
     3541 ctcgaaggcg actcgcccgt tcgcgatcag gtaatagccg ggatccgcgc ggcgatccgt
     3601 cgctgtgtcc acctcctgcg ctgtgccgcg ggagcgtttc gcttgaaaaa ctgccagccg
     3661 ggcaacttca cgctcatcgt gcggcgagcc ccgcgagagt tcttcgatgg cgcgccggta
     3721 acgatctcgt gtcacgaagt ccggagcggc aaaatcggtg tcgatccaca gtatggcgtc
     3781 caccagactg acgctctcaa agaattccgc ccagtcgaag gcggaaatca gacgcatgct
     3841 ggtgatcacg ttgcgcacgg tcacgttcat cgcagcttgc cgttggtgct cgacccggac
     3901 aatctcatcc gccgtcgtgc cctgcgccgc caggcgttgc tcgagccagc gcagcgccgg
     3961 catgatcacc gggtcctgat cgcgcagtcg ctggacaagc tgtacggcga aggctgtcga
     4021 caatgaggcg ccctcgaacg gccggagcac cgtcgccgct cgctcgattg aactaccgcc
     4081 aactccgagc aggcggtcgg ccaccgcgtc ggcttcctgg cgcgccgccc gttcatgcac
     4141 catgcgctcc gccaggcgcc ggaggttctc gagcagcacc acgcgcagcg taatcgctac
     4201 tgcccagagc tcgccaatcg ttaatggttg cacgcgctgg taggcgcgca cgaagcgacg
     4261 cagcatctcc gggtcaaacc ggctgtccgt atgggcgatg aaggcccagg cgagcccaaa
     4321 gacgcgcgga taaccctgca aatgaccctc ggccagctta ggcagttctc ggtaatagcc
     4381 ggacggcagg tcatcgtgaa tctcgcgcaa ttgttcgtcg atgatgtgat aattgtcgac
     4441 cagccactcg gccgcaggtg tgatggcacg ctcctcgcgg actgcctggg cgattgtacg
     4501 atacgattca agcagcactc gcccgttgtc gacgacccgc ggcaacagcg gacgaccctt
     4561 gcgcggcgtc atcgtgacgc gctgcgccac agcgaggctt tcgccgtgct gctcgagacg
     4621 ctcgctgcta aagagttcgg cccgaatcgg ttcttcgagc tgtgaaagtg cgatggcttc
     4681 tgaatgtttt ttgcgtaata atttctgacg gaatggcaat ttcaatccat atacctcacg
     4741 cctattatcg cgcgcgtcgg gcgcataaac acgctatcaa gttcggcagt cgtgaggaat
     4801 atgcagggca gggaatgttg aacaaacgcg attctggggc tttttttgtc gtggccaaca
     4861 agccgtagct cggcaatcat agcgctctcc tttgcgctcc actccgaata agggacccct
     4921 atttgcttca ccagtcgcct tcgtcgccgc agccgtctct catcgttggc cccatttcgt
     4981 ataaacggag ctcagcggaa tatctccctc acggtggatc gcgacaagca ccggttccct
     5041 tcaggcgaga gcatgatcta cgcgctccca atgaagtaga ccatctcgtc ttgatcgaca
     5101 gcttacttga tagcaacttg cgctatcggg tttaaccgtc gtcttcgcag attcgttacg
     5161 cgtgcgaatg gcgatcgtgc cgacgctgcg atcgcgttag cagcgcgttc agaatcacca
     5221 tcaacagggt taagagcact aaatagaaga atagactttc agttaacata ctccacctcc
     5281 gcttcggaga agttacaatt gaagcaagcg caaggcggat gccatctcgc ggccgccgta
     5341 ggaccgccgc ggcgtgacaa atgcctgtgc gatgcgcgcg ttacatgaca gcagtaatcc
     5401 gccgcactaa agctattccc agctatgaga aagataatgg caaggtcgag aagacgccgc
     5461 ccatgtcgat aagtcgctgc ggggcgatcc gaaaaacacg ggacagcaac gcatgcctcc
     5521 attaactgaa gtcgaacaga atcttcaggc ccacatgatc gagaagcaac ggctcgccga
     5581 gtctatttac tgttacgtct ttaccgagcg ctcatcgaga atcccgacct atcctccggc
     5641 cttcgcgccg cgcccgtaat ccggcatgaa aagcgtatca ccgacgaggc aaacgggacg
     5701 gtttatttga aatcccattg aacgcgtttt gagacgcgag cgacctcttc aatcttcgaa
     5761 cccgaaactt acgaccaaca gagttcaact gcgagcagga gcgatggaaa gtgcacgaca
     5821 ttgaggccgg gcgatttcgt tacgcgcgat ttcctgattt taaatcgcca cggtttgact
     5881 ttatggtctg ctcttcgctc acaatgtttg ttggtcaaaa catgaacgcg ttattgacgg
     5941 cgcagttatc cgggacacgg caatcgcaga actgggatac tgcaaattcg tttttttcag
     6001 tactgggaag ctgcgactta aaacgtgctg cattgttggc tccttcgctg gcatatcggt
     6061 tgcggtagca tttgttgaag cagccaaata agacaaagaa ggagggcaaa ataatggcag
     6121 agaagaataa agaaaaagaa atgaaggcgg tgtctccatg gcgacccttc agggatctca
     6181 ccagctggga cagagatatg gagagaatca tggaggattt cttcggcagg cggatgcggc
     6241 cgtggtggcc ggagcgatgg tctagaactg aggaaatgga ggtaaaaccc ccgatcgttg
     6301 atgtcttcga agagaaggac gatattgtcg ttaaggccga gcttcccggc atcgaaaaag
     6361 acaatatcga ggtgaatctg acggatcaca cgctgaccat caaaggcgaa aagaaaaaag
     6421 aagaagaggt gaaagaagaa gactattacc gcgccgagcg ttcgtacggg agctttataa
     6481 ggtcgttgga tctgccaaga gatgttcgcc cagaccaggt gaaagcctct ttcaagaacg
     6541 gcattctgga agtaaggatg cccaagaccg aagaggcgaa ggccaaagaa atcaaagtga
     6601 aagtcgagta gcgaaacggc gcggaagcgt gggggcaagc ggattttgac gagtggcgag
     6661 gaggaagcat gtacagcaaa atactagtgc cgttagacgg atcgaagacg gcagaaaacg
     6721 tgttgccttt ggctcgttcg tttgcccgcg ctcttcaaat ccccgtggag ttgctgggcg
     6781 ttatcgatat agtcgagatg gcataccacg tttccaccga tcgggttccg atgattcgct
     6841 cgatgattga cgatgccagc cgaagatccg ccgagtattt ggaaggaatt gcccagaatt
     6901 ttccgaccgg cacgacgcgt tggacggtcc agaccggaag tgctgccgag gctattatca
     6961 attctgctgc cacaaataaa caaacgctaa tcgcgctggc aacccatggt cgctccggtc
     7021 tcaatcgctg gttgctcggc agcgtcgcgg aaaagatttt gcgaggcggc tccaatccga
     7081 cactggtggt tcgagctcgg cacgacaaga atccgagctg ggcaatggcc acgttgaagc
     7141 gaattattgt tccactcgat ggctccgaga tgtccgaggg tattttgccg tatgtcaaag
     7201 agctggcgaa acagcttgat ctggaaataa agctgatccg cctctacggc gggttatcgg
     7261 ccgccggcga tggtttttat aatccccatc agttagatgc gttgactggc aggctccgtg
     7321 aagaaaccgt ggcatatctc gaaagtaaaa cagacgagat gaagcaacag gggcttaaca
     7381 ggatctcctt cgcagctaag gagggtctgg aaccagacga aattctggcg attgcgcggg
     7441 aaaccccaga tacgatgatc gctatgtgca cgcatgggcg ttccggcgtg aagcgctggg
     7501 cgctaggcag cgttaccgaa cgggtcgtgc gccattccga taacccggtt ttggttgtgc
     7561 gggggtcttg atcaactgcc tctcgggagg gcgccgaacg atgtacagca aaattctcgt
     7621 gcctctggat gggtcacgta cttcggagcc gattttgctc tatgcccgtt tgtttgccga
     7681 tgcgtatggc atccccgtgg agttgttgcg cgtcaacgat ccagacgcgc gtccgccgtt
     7741 ttggccgtcc gaaagcagcg ggcgctatct aaagaaaatt gccgatgaat atttctccgg
     7801 tcgcgctagc accagcgctg aagccggcgc accggccgcg tttattgtcg atcatgccaa
     7861 gaccgatcct tcgtgcctga tcgccatggc gacacatggc ctctccggag cgcggcgttg
     7921 gctgctcggc agcgtgacca gcaaagtcgt tcaaaccgcg acgaatgctc tgctgctgat
     7981 ccggcccagc cataacttcg atgcttcgag gccggtcagg atggatcaaa tatttgtccc
     8041 gctcgatgga tccgctttag cggagaaagc cctgccgcat gcgtgcgcgc tcgccaaaag
     8101 actgaacccc gagattcaat tgctgcgtgt ttattcgcta ccgatggacg cctacgtcgt
     8161 tgccgatggc gtaatcgctc gcggtccggc gcagtatcgc gaggaattgg ccaagcaagc
     8221 gcaggattac cttgaaggca aagttgcagc cctcagggcg gacgggttgg agcgcgtcgt
     8281 cgggatcgcg ctcgaaggcg atgcggccag cgaaattatt gatcttgcat ctaaaacgcc
     8341 ggacagttta attgcgatgt cgacgcacgg ccggtctgga attggccggt gggtcctggg
     8401 aagtgtagcg gagaagattc ttcagcatgc gcacaacccc ttactgctga ttcgagcgaa
     8461 ttgaaagtca ctgaaaccgc ccaagcgctg caccggcgcc ttgcgcgtgc cgaggcaatc
     8521 ctgatgatat tctccggatc aattcggctg gcgtgaagag tcgatctttg tgcagctgaa
     8581 agaaaggatc tttccatgtc aatgttgaag acaatactgg cgccgacgga tttttccgag
     8641 gtctcagcct ccggcgttcg ctacgcgtgc caattggcga aagatgtagg cgctgagatt
     8701 atcatcttca atgtggatgt attggacgag agcaaccggg tcgacaagcg cgagatcgaa
     8761 caacacaaac gtcggctcgc cgaatttgtc accgagaaag tccccgacgc ggcggttggg
     8821 ctgaacatgc gccaactcgt ggatgctggc cagccgttcg gcgccattgt cgactgcgct
     8881 gaaaaggagg cggtcgactt aatcgtcatg tcttcgcacg gccgcagcgg cttatcgcgc
     8941 atgctgatcg gcagcgttac ggataagatc ttgcgtggag cgccgtgtcc ggtcctggtc
     9001 gtgccattgg aacaaaagaa aagctaacga caaaactgta cccttacttt cctcctcggt
     9061 tggcgctgcg ggcggattac ttcgccgccg atcgagcctg ccggaatggc ggcgacggat
     9121 ggcgctttct ggtgacctta cttccaaagt aacgtaggac gttggtagtg gtgactagtc
     9181 ccaccagcgc tccgttactg agtaccggca gacaaccgat cttcttctcc gccatcaccc
     9241 gggcggcaac ttcaactgga gtatcgggcg tcaccgtact aacccttttt ttcataatgt
     9301 tcttaatcaa ctgagtcttg agcaaagccg atctggttgc ccgcttaaga ccaaagatgc
     9361 ggtctgacgc ggctcccatc agatctcgct cagagatcaa gcctaccaac ttgccgtttt
     9421 cgagcaccgg gatatgacgg atgcgcccaa gagaaatcac gtcattggcc agatctagag
     9481 tatcgtttgg ggccaacgtt acaggcgacc ccatcataat ctcgctgaca gttcctgcct
     9541 tcggcttttt cataggagat tctaagcctt tctttcgcgg cggtggcgaa accattcgtg
     9601 ccgtcttgcc acgggtggag cactgtccgt gccgatcttc atgagaattt tctttgcgag
     9661 aaccgggaaa cgcaagttct tccggttccg ccgcatttgc gcagaaataa tctccccaag
     9721 cattttagtc cgctaattca catccgcgat cttcttgatt ccatcccccc aatcttcaac
     9781 tttaaagtcg gagaacgaaa gcacgatcgg ctctttttcc tcgatcacga tcatccggtt
     9841 tttgtacttc atcaggaagg agattttgag caataacgag tcgtcgcgaa attcgagaag
     9901 cgtgaccttt tgctcgaaat aggagtattg ctgctcgtgc aatatctctt cgactcgttt
     9961 tcgggtaagc gtctctttag gagctaaaaa accgtccatc ttccctccac attcctgttt
    10021 gccggcgttt ctgaagtttc aatctcgcaa tccttccttt cagagcgttc tcatgcctgc
    10081 tcatcctcca aacggtcgag aaaacagcgc aggacgtcca cataggtgac gatgccgacg
    10141 aggccttcgg tttcatcgac gacgggaacg ccgccgatct tttcttcgag cagcaattcg
    10201 acggcttcgc ggagatcgtt ctcaggggat agtgtcagcg gcttggcggt cattatgtca
    10261 gtgaccttgg ttctgagggc tttgccgcgc ttctcaggct caagaagcga tgattgagca
    10321 aggaaagaac ggatgtcgcg atccgtgacg atgccaatga gctgcttatt ttcgaccaca
    10381 ggtagctgtc gataatggtt ctcgtacatt agctcttcgg cttgtcctac cgtgtctgaa
    10441 gcactcacgg tcaaagggtc cttggacatc acatcgttta catttatcgc attaaatttc
    10501 atgatgattc acttcatcct tttatccggt atcgcacaat ttctaggcag cacagggtat
    10561 gccaagtacg ttaagataaa tgggggtcat gcgcccaccc gtagcggcat cgatcactaa
    10621 atttttccct tccatgcgaa aaaatcctat cgcgattcca agcaatgcga gacaaaagtt
    10681 tgttcgcgta agaaatagct actttctcca ataggagcag cggcatagtt attgcgctta
    10741 tcctattcat cgttcgactc aagtcaggag gttttgcatg gatccaataa agaaaattct
    10801 cgcgccgacg gacctctcgg acctttcaag cgcgggtttg cgttatgcgc tggagatggg
    10861 gcgatcacgc ggcgcagagg taatcgtcta tcacgtcatc gacctaggcg acaagtgggc
    10921 tgacaggcgt gaggagtccg ggccagtgcg tgaaatgctt gagcgccata gacgcatgct
    10981 ggaaagattt ctatggaaga actttccgga ggagatgaat ctaaccgaag tgcggcaggt
    11041 tgttgagttc ggcggggccg tcgagaacat catcgaaaag gccgaaggtg agcgggtgga
    11101 taggatcgtt atgtcgaccc atgggagaac cggcatcgat cacctgctga tcggcagcgt
    11161 ttccgagaag gtcatcgcgc gagccccatg tccggtcttg attgtgccgg cgcccaggcg
    11221 caatctcgca agggctgcat aattggcgca atcattgggc gtgatggcgg tagataaagt
    11281 aataaaccaa cgctaccatc acgccgcccc cgaatatgtt gcccaatgtg acgggtacca
    11341 agttagcgat caagccgccc caggttagca gttccgcatt tgccgcgtca gagttctctc
    11401 gtaatagaat cccgagaggt atgaaataca tgttggccac gctgtgctcg aaacctgcgg
    11461 cgacaaacgc g
//
LOCUS       DASUVS010000007         8980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_875316, whole genome shotgun sequence.
ACCESSION   DASUVS010000007 DASUVS010000000
VERSION     DASUVS010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8980
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875316"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VF182_00225"
     CDS             complement(<1..565)
                     /locus_tag="VF182_00225"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6435526.1"
                     /translation="MFEDLRGFLAHLESQKQLLRVKDEVDVKYEIAAGMRKTSDIEGP
                     ALLFENVRGFRGWRVLGGLFATRKLVALGLGVPQEQMLERYLTLEDKRIPPEVISDAP
                     CKEIRWTGDQVDLSKLPIVTHAGKDCGPYVTIGVQIGKNPDNGIRNLSIHRMLVLGKD
                     RLSLWAPADHHLGRMILMAEEKNKGLEV"
     gene            complement(680..1663)
                     /locus_tag="VF182_00230"
     CDS             complement(680..1663)
                     /locus_tag="VF182_00230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6435527.1"
                     /translation="MRKLPIKWGISLPNRGALFGLTDVDELIETAKLAEHSGVFESVW
                     FGDSLIHKPRLEAITMLAAVATHTKKVRLGTICMASFPVRHPVLLAIQWASLDQISNG
                     RTLLGVCIGGGHEGELRAFGVKREERVGRMKEGIELLRQIWSDKEVMHKGKYYTLEGY
                     NIVPKPVQKPPPIWIAVSPEREQAGDKLVDQAMRRVGALADGYITMGVTAEELGKRLN
                     VIEQSAKETGKDLSNFEVAIHGMVNINDDKHAAYAQAKDYFNHYYGPAYPPESLIKVW
                     LAHGPPKDCAQLIQQWIDMGITTPVLRFTAKDQLEQVSRFIDEVVPLLRLN"
     gene            1960..3393
                     /locus_tag="VF182_00235"
     CDS             1960..3393
                     /locus_tag="VF182_00235"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6435528.1"
                     /translation="MSKDFRSYLDELLEKRPKDVVVVDKQVDPRYEASAIVEKFERKN
                     EFPLVFFKNIKGSQIPLVINLGATYERLALSLGSPSVPQMVRDLAHREHNPVPVKEIS
                     PKEAPCKEVILKGEAADLDLLPVLTLNEGDAGAYVNAAAMICRERGTGAVNVGIYRHQ
                     KQGKRQLGLMVNPANHANYVRAEYEDHNEPMEVALAIGHHPALNMAAVSKQAGVGSEL
                     EIAGAFLGEALEVVKAETVNLMVPARAEIIIEGIVPPKKRHYEGPFGEWPHYYYKEGE
                     QPYIEVTAITMRKNPIYQCIHSAHFEHNIIGAAPRLGSLLKRIQEVVPSTTGVNMPIS
                     GAARSHCYISVKKRAEGDPKQAALAAIVTDPNIKHVIVVDDDVDVFNETQVLWALAMR
                     FQADRDLVIIPNIIGSHLNPTAYGYNRLEKGPMETKLIFDATKPLPPYHFPKEAQAPD
                     EVLNKIDLARDTRPYEPGKDDKAITGG"
     gene            3398..4297
                     /locus_tag="VF182_00240"
     CDS             3398..4297
                     /locus_tag="VF182_00240"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013020807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6435529.1"
                     /translation="MVQNVQAVQDLQNVEELPGGLSGLNVVNDINQAKSLVEIQGVSK
                     SFHKTVKESTTEIKALADVSLSIRENEFVSIIGPSGCGKTTLLKMIDGLLPYDSGRIL
                     IDGRQVSAPGPDRAVVFQAFALLPWRTVLANVEFSLELRRLPKEERTQTAREYLSRVG
                     LADFENHYPHELSGGMQQRAGLARALAVNPAILLMDEPFGAVDAQTRQLLQEELLELW
                     QSQRKTVIFITHSMDEAVYLSDRVVVMTPRPGKVAEILDVPLPRPRVSEAVRRDAKFV
                     DLTNYIWESLKKAMENHPIEQPF"
     gene            4281..5036
                     /locus_tag="VF182_00245"
     CDS             4281..5036
                     /locus_tag="VF182_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6435530.1"
                     /translation="MNNRFSANLLSILSLAVLWEAVGRAMDSTLIPPLTQIGAAWWKL
                     LSSGKLLANLGMSLTTLAIGFCLAAFVGVVLGVLMGRFRAIEHFFDLYVNALMSAPTT
                     AFVPVLILWFGLGMESRIAVVFLFAIFVIIINTMTGVKQVDTVLLEMARSFGAREREI
                     FFKIMLPAALPAIMAGLRLGMGRAVKGMVTAEMLLTLTGIGAMIMQYGSSFATDSLFA
                     VILTILLVALLAMKAVHWIDRRLTGWKAEIAIE"
     gene            5060..6043
                     /locus_tag="VF182_00250"
     CDS             5060..6043
                     /locus_tag="VF182_00250"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435531.1"
                     /translation="MGRLSREILGLLTILTFGLLPAGQAQAQKKVRFAYPSSADMGDI
                     PSLLAWEQLKKQGIEVTPTFFPKTDLAVQAVVAGEADIGSAAGIAVVKAVESGLNIRI
                     IAEQVRNEWQLVTPVAFKDPKQLNGKRVAYHAPITVTEALVKWMAQHYKITPNWMIIP
                     GSDVRAEALMRGQLDATPAEIGDVLNILSAKPGQFHVLVSYAKTFPQLIGSMYFARAD
                     YVQKNAATVESVLEAILRAHRDADERPASVKENALRLLPETKPELVDAIAATYKELKI
                     WDVNGGAGKERGEASIKFFEESGLLKKGAVSVNQSFATAPLEKVLAKIGRK"
     gene            6046..6789
                     /locus_tag="VF182_00255"
     CDS             6046..6789
                     /locus_tag="VF182_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6435532.1"
                     /translation="MKASTLRIISLSSLAAIWEIAGRSGNAHLLPPLSQVISVWAELL
                     VSGQLFQAIAVSFQALAIGFFISVVFGVPLGLLMGRFRRLESFLDVYMTALLAVPMIS
                     FIPFLVIAFGLGLQSRVWIVFLFAFVIIVINTTAGVRSVDPTLTEMARSFGARESELF
                     LKIILPAAMPMIMAGIRLGMGRAVLGMVTGEMILSVVGFGAMLMTFSASFNSSALFAT
                     ILTIVLLAILLLALIQYLDRRLMPWRAAA"
     gene            6917..7924
                     /locus_tag="VF182_00260"
     CDS             6917..7924
                     /locus_tag="VF182_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435533.1"
                     /translation="MINLFVRSFIAFLCLPLVLPMSYSFVSAQSQSERLPVAYTVIAP
                     TQATLWTAKELGFFAKNGLDVELVLLVGAPLAVTALVSGETPIVHAGASAVVTSNLQG
                     SGAVLVAGGANRFPYVLFVTDEIKRVEDLRGKKFGVSRIGSADNAAAITVLNRFGIKE
                     TDLTYVQAGSIPARLAAMQTNALQATLLQAPETLKAKEIGMRPLLDFTKLDVEWQQNG
                     VATTRDYIKKKPETVRRFIRAFVEGSAYNLNNPKGAQKILQKYLAIKDDKSVQESYNE
                     IVAKLTLKVPYPTEPGLQLYLDQLKIKNPKAAQAKPSDFTDVSFLKELETSGFIDRLY
                     K"
     gene            complement(8042..8677)
                     /locus_tag="VF182_00265"
     CDS             complement(8042..8677)
                     /locus_tag="VF182_00265"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HEX6435534.1"
                     /translation="MKIISETIVKSNSGQTFEVKKGQIIRVIGESTADYVVFNLRNVK
                     ERFDQARTKVDQGKIFVTTGDVLVSKFNNVMQTIVKDTYRGTHDMEKGMCSTSFYKKW
                     GDEIFKIYGGVWKKLGRKKVVAPKHGCWENLARALKPHGVPMEDVPSPLNIFQTMVIN
                     AKTGSMRYSMTRPRPGGDMMDLRSEMDCLVGISACPEGGRGKDLKIVIYKN"
BASE COUNT         1997 a         2521 c         2424 g         2038 t
ORIGIN      
        1 ccacttctaa gcctttgttc ttttcctcag ccatcaggat catccggcca aggtgatgat
       61 cggctggcgc ccagagagac aggcgatctt taccgagcac gagcatgcgg tgaatcgata
      121 aattgcgaat gccgttgtcg ggattcttgc cgatctgcac gccgattgta acgtaaggtc
      181 cgcagtcttt gccggcgtgg gtgacgatcg gcaatttgga caaatcgact tgatcgccgg
      241 tccatcttat ttctttacag ggcgcatccg aaataacctc gggtggaatg cgcttgtcct
      301 cgagagtcag gtaacgctcg agcatctgct cttgcggcac cccgagaccg agagccacca
      361 attttctcgt cgcaaagagt ccacccagga cgcgccagcc gcggaagcct ctaacatttt
      421 caaatagaag ggccgggcct tcgatatcgg aggtcttgcg catcccagcg gcgatctcgt
      481 acttgacatc gacttcatct ttgacgcgga gtaactgctt ttggctttcc agatgcgcca
      541 gaaatcctct aagatcttca aacatggcgg ttcctctata agattggagt actggagtgt
      601 tgaagcgatg ggtttcggca ccggatcatt ccattgcttc actactgcat cactccagtc
      661 tttatcaatc atcgtgccgt cagttcaacc gcaacaacgg caccacctcg tcgataaatc
      721 tgctcacctg ctcgagctga tcctttgccg taaatcgaag taccggtgtg gtgatgccca
      781 tgtcgatcca ttgttgaatc aattgggcac agtctttggg tgggccatgc gcaagccaaa
      841 cttttatcaa gctttccggc ggataggccg ggccgtaata atgattgaag taatcttttg
      901 cttgggcgta ggctgcgtgt ttatcgtcgt tgatattgac catgccatga atcgccactt
      961 cgaagttgga caggtctttg ccggtctcct tggcggattg ctcaataaca tttaaccgct
     1021 tgcccagctc ttcagccgta actcccatgg taatgtagcc atcggcgagc gcgcccaccc
     1081 ggcgcattgc ttgatcaacg agcttatcgc cggcttgctc gcgctccggg ctgacggcga
     1141 tccaaatcgg cggcggcttc tgcaccggtt taggcacgat gttgtagcct tccagcgtat
     1201 agtacttgcc cttgtgcatg acctctttgt cgctccagat ttgccgcagc aactcgatcc
     1261 cttccttcat gcgccccacc cgttcctcac gcttgacacc gaaggccctg agctccccct
     1321 catgaccgcc gccgatgcac acgcccagca gagttctacc atttgagatc tgatcgagtg
     1381 aagcccactg aatcgcgagc agaaccggat ggcgcacggg aaaagaagcc atgcagattg
     1441 tgccgagtcg aacttttttg gtgtgagtcg ccaccgctgc gagcatcgtg atcgcttcca
     1501 gccgcggctt gtgaatcaag ctgtcgccga accagacgga ctcgaagaca ccggaatgct
     1561 cggccagttt ggctgtttcg atcagctcat cgacatcggt gagcccgaac aacgcgccgc
     1621 gattcggaag actgataccc cattttatcg gcagctttcg catcctgctt ccctgtccac
     1681 tgttaagaga acgccaatga gctgtcaagc aaccgtccgg taatgcattg gttcatcttc
     1741 acacaaattt gtaggggcaa cccccggtgg tggccctaag cagggcaggc atagcgcctg
     1801 cacctatata agacgccgtc gggacaaatg cacagacctc aggcagcgtt cggcttgaca
     1861 ggcgcgtgcg attgcgcgca tagcataaat gcatcagccg ggtgaccagc ggtcactggc
     1921 cgcattgacg atcgaaagct aagccggagg tgagccaaaa tgtcaaaaga ttttcgcagt
     1981 tatctggacg agctattgga aaagcgcccc aaggatgtcg tcgtcgtcga taagcaagtc
     2041 gatccgcgat acgaagccag cgcgatcgtc gagaagtttg aacgaaaaaa tgaattccct
     2101 ttggtttttt tcaagaacat caagggctcg cagattccct tggttatcaa tctcggcgcc
     2161 acctatgaac gcctggcact atcgctgggc tcgccatcgg tgccgcagat ggtccgagat
     2221 ttagcgcatc gcgagcacaa cccggttccg gtcaaagaga tttctcccaa agaagcgcct
     2281 tgcaaagaag taattctgaa aggcgaagct gccgatctcg atcttctacc ggttctgact
     2341 ctcaacgaag gcgatgccgg cgcctacgta aatgctgctg cgatgatttg ccgggagcgc
     2401 ggcacaggcg cggtcaatgt cggcatctac cgtcaccaaa agcagggtaa aagacagctc
     2461 ggcctgatgg tgaacccggc caatcacgcg aactacgtcc gggccgaata cgaagatcat
     2521 aacgaaccga tggaagtcgc tctcgccatc ggccatcatc ccgcgttgaa tatggcggca
     2581 gtcagcaaac aagccggcgt cggctcggaa ctggagatcg ccggggcatt tttgggcgag
     2641 gcgcttgaag tcgtcaaggc cgagacggtg aacttgatgg tgccggcccg cgccgagatc
     2701 attatcgaag gtatcgtgcc gccgaaaaag cgtcattacg aaggcccctt cggcgaatgg
     2761 ccgcattatt actataagga aggcgagcag ccgtacatcg aagtgaccgc catcaccatg
     2821 cggaagaatc cgatctatca gtgcatccac agcgcgcact tcgagcacaa tatcatcggc
     2881 gccgcgcctc gattgggcag cctgctcaag cgcattcagg aagtcgtgcc gagcaccacc
     2941 ggtgtcaata tgccgatatc tggcgcggcc cggtcgcatt gttacatctc ggtcaagaag
     3001 agagctgaag gtgatcccaa acaggcggcc ctcgcggcca tcgttaccga tccaaatatc
     3061 aaacacgtga ttgtcgtcga cgacgatgtc gatgtgttca acgaaaccca agtgctttgg
     3121 gccttggcta tgcgctttca agcggatcgc gacctggtaa tcattcccaa catcatcggt
     3181 tcgcatttaa atcccaccgc gtacggttac aatcgtctgg agaagggtcc catggaaacc
     3241 aaattgatct tcgatgccac caagccgctt ccgccgtacc atttccccaa agaagctcag
     3301 gcgcctgatg aagtgctcaa caagatcgat cttgcgcggg atacgcggcc gtacgagcca
     3361 ggcaaagacg ataaggccat cacgggcgga tgaagcaatg gttcaaaacg ttcaagcagt
     3421 tcaagacctt caaaacgtcg aagaactgcc cggaggttta tccggcttga acgtcgtgaa
     3481 cgatataaac caagcgaagt ctttggttga gatccagggg gtttcgaaga gctttcacaa
     3541 aaccgtaaaa gagagcacga cggagattaa ggcgttggcg gatgtgagtc tttcgatccg
     3601 cgagaacgag tttgttagca tcatcgggcc gagcggctgc ggcaaaacca ccctgcttaa
     3661 aatgattgac ggcctgctcc cgtacgatag cggcaggatt ctcatcgacg gcaggcaggt
     3721 gagcgcgccg ggacccgacc gtgccgtcgt atttcaagcc tttgctctgt tgccctggcg
     3781 taccgtcctc gccaacgtcg aattcagcct cgaattgcga cgattgccaa aagaggaacg
     3841 gacacaaacc gcgcgcgagt atctaagccg ggtgggtttg gccgacttcg aaaatcatta
     3901 cccccacgag ctctccggcg gcatgcagca acgcgccggc ttggcccgcg ctctggcagt
     3961 caatccggcc atcctgctga tggacgagcc tttcggcgct gtcgacgccc aaacgcgcca
     4021 actcttacaa gaagaactgt tggagctctg gcagagccag cgcaagactg tgatcttcat
     4081 cacccacagc atggatgaag cggtctatct ttcggaccgg gtcgtggtga tgactccgcg
     4141 tcccggaaaa gtggcggaaa ttctcgatgt gccgctgccg cggccgcgtg tctccgaagc
     4201 ggtccgacgc gatgccaaat tcgtcgactt gaccaattac atttgggaga gtctcaagaa
     4261 ggcgatggag aaccacccta ttgaacaacc gttttagcgc taatcttctg tcgattcttt
     4321 ccctcgcggt cttatgggag gccgtcgggc gagcgatgga ttcgaccttg attccgccat
     4381 taacgcaaat cggcgccgca tggtggaagc tgttgagcag cggcaaactg ctagccaatc
     4441 tcggcatgag cctcacgacg ctcgccatcg gcttctgcct tgccgcattc gtcggcgttg
     4501 ttctcggcgt cctcatggga cgttttcgcg ccatcgaaca tttctttgat ctctatgtaa
     4561 acgccctcat gtcagcgccg accactgcat tcgtaccggt gcttatcctg tggttcggac
     4621 tcggaatgga atcgcgcatc gccgtggttt ttcttttcgc catattcgtc attatcatca
     4681 acaccatgac cggcgtgaaa caggtggata ccgttctgct ggagatggcg cgctccttcg
     4741 gcgcgagaga aagagaaatc tttttcaaga tcatgctccc cgcggccctg ccggcgatta
     4801 tggccggtct tcgattgggt atgggacgag ccgtcaaggg catggtaact gcggagatgc
     4861 tgctcacgct taccggcatc ggcgccatga tcatgcaata tggatcatcg ttcgccaccg
     4921 actcactctt cgctgtcatt cttacaattt tgctggtggc gctgctggcc atgaaagcgg
     4981 tgcactggat agaccgccgg ctaaccggat ggaaggcaga aatcgctatc gaataggtcg
     5041 tgaaatacga ggggggcata tgggcagatt aagccgcgaa attctgggac tgttgacgat
     5101 tctgactttc ggattgcttc cggccggcca ggcgcaagcc caaaagaagg tgcgattcgc
     5161 ttatccctca tcggcggata tgggagacat tccttcgtta ctcgcctggg agcagctcaa
     5221 gaaacagggg atagaagtga cgccgacatt ctttcctaaa accgatttgg ccgttcaggc
     5281 cgtggttgcc ggcgaagccg atatcggttc cgcggcgggt atcgcggttg tgaaggcagt
     5341 cgagagcggc ctgaatatcc ggatcatcgc cgagcaggtg cgtaacgaat ggcagctggt
     5401 cacgccggta gcttttaagg atcccaaaca attgaacggc aagcgggtcg cttaccacgc
     5461 gccgattacg gttaccgaag cgctcgtcaa atggatggcg caacactaca agattacacc
     5521 caattggatg atcatcccgg gatccgacgt gcgcgcagaa gccttgatgc gcgggcaatt
     5581 ggatgcaacg ccggcggaga taggcgacgt attaaacatc cttagcgcga agcccggtca
     5641 gtttcacgtg ctggtctcct atgcgaaaac ctttccacag ctcatagggt cgatgtattt
     5701 cgcgcgcgcc gattacgtgc aaaagaacgc tgccacggtt gaaagcgtac tcgaggcgat
     5761 tctcagggcg catcgcgacg ccgacgagcg cccggcgtcg gtcaaagaaa atgctttgcg
     5821 actactgccc gaaaccaaac cggagctcgt cgatgccatt gccgccactt acaaagagct
     5881 aaaaatttgg gatgtcaacg gcggcgccgg aaaggagcgc ggcgaggcga gcatcaaatt
     5941 tttcgaggaa tccggactac tcaaaaaagg cgccgtaagc gtcaatcaat ccttcgccac
     6001 ggcaccgttg gaaaaagtgc tggcaaagat cggacgcaaa taagaatgaa ggcgagcacg
     6061 ctcagaatca tctcgctctc gagcctcgcc gcgatctggg aaattgccgg gcgcagcggc
     6121 aacgctcatc tgctgccgcc gctgtcgcaa gtcatcagcg tttgggcgga actgctcgtc
     6181 tccgggcagc tctttcaagc gattgcggta agctttcagg cgcttgctat cggttttttt
     6241 atttccgtag tgttcggcgt gccgcttgga ctgctcatgg gccgcttccg gcggctcgaa
     6301 tcgttcctcg acgtttacat gacggcgctg cttgctgtgc cgatgatttc gtttatcccg
     6361 tttctagtga ttgctttcgg tttaggtctc cagtcccgtg tttggatcgt tttcctcttc
     6421 gcctttgtga ttatcgtgat caataccacc gctggcgtgc gcagtgtcga tccgaccttg
     6481 actgagatgg ctcgctcctt cggcgcccgc gaaagcgagt tatttttaaa gattattctg
     6541 cccgctgcca tgccgatgat tatggcgggc attcgcctcg gcatgggccg tgcggtgctc
     6601 ggcatggtaa cgggagaaat gattctttcc gtcgtcgggt tcggggccat gctgatgact
     6661 tttagcgcct cgttcaattc gagcgcgctt ttcgcgacca tcctcacgat cgtcttgctg
     6721 gcgatcctat tattggcatt gattcaatat ctcgatcggc gcctcatgcc ctggcgcgca
     6781 gcggcctaac catggggcag gcgtcaaaga cctgttcacg ccattgatat cgccggaagc
     6841 ttaagatatt tgcccgccta ggcgacatca tcaccttgac actagccgct gaagttttgt
     6901 ccagaggaga atcgtcatga taaatctctt tgtccgatca ttcattgcat ttctttgcct
     6961 gcccttggtc ctgccgatga gctactcatt cgtcagcgcg caaagtcaat cggaaagact
     7021 ccccgtcgcc tataccgtca tcgctccgac gcaagcgacc ttgtggaccg ccaaggaact
     7081 cggcttcttc gccaagaacg ggctcgatgt cgaactggtt ctcctggtcg gcgcgccgct
     7141 tgccgtcacc gccctggtct ccggggaaac tccgatcgtt cacgccggcg cctccgctgt
     7201 tgttaccagc aacctgcaag gatcaggcgc ggtgttggtt gccggcggag ccaatcgctt
     7261 tccctacgtg ctttttgtca ccgatgaaat caaacgggtg gaagatctga gaggtaagaa
     7321 attcggcgtg agccgcatcg gcagcgcgga caatgcggcg gcgatcacgg tgctgaacag
     7381 attcggcata aaggaaaccg acctgacgta cgtgcaagcc ggctcgattc cggcgcgcct
     7441 ggcggcgatg caaaccaacg cgctgcaagc gacactgttg caggcgcccg agacgctaaa
     7501 agccaaagag atcggcatgc gccccttgct cgatttcacc aagctcgatg tcgaatggca
     7561 gcaaaacggc gtggcgacga ctcgggatta tatcaagaaa aaacccgaaa cagtgcgccg
     7621 ctttatcaga gctttcgtcg agggcagcgc ttacaatttg aataatccca aaggcgcgca
     7681 gaagatcttg cagaaatatc tcgcgatcaa agatgataag tccgtgcaag aatcttataa
     7741 cgagatcgtc gccaagctaa cgctcaaagt tccctatccc accgagcccg gcctccagct
     7801 ctacctcgac cagttgaaga tcaagaatcc caaagccgcg caagccaagc cgagcgattt
     7861 taccgacgtc agctttttga aagaactgga aaccagcggg tttatcgaca gattatataa
     7921 atgaagatag ctccaatcgt tccattcgtt tcatccccct cccttcaacc tctcccgcga
     7981 cgcgggggag gaaagaggtg ggggtttcaa cgcagcgcga gcggttgaac ggcttgaacc
     8041 atcagttctt gtagatgact attttgaggt ccttcccccg cccgccttcc ggacaagcgc
     8101 tgatgccgac caggcaatcc atttcggaac gtagatccat catgtcgccg ccgggccgcg
     8161 gccgcgtcat cgaataacgc atgctgccag tcttggcgtt aatcaccatt gtctgaaaga
     8221 tattgagcgg gcttggcacg tcctccatcg gcacaccgtg cggcttcaaa gccctggcga
     8281 gattttccca gcagccgtgc ttcggcgcaa cgactttttt tcttcccaat tttttccaaa
     8341 cgccgccata tatcttgaaa atttcatcgc cccatttttt gtagaaagac gtgctgcaca
     8401 tgcctttctc catgtcgtga gtgccgcggt aagtgtcttt gacgatggtt tgcatgacgt
     8461 tgttaaattt cgagaccaac acgtccccgg tcgtcacgaa aattttcccc tgatcgacct
     8521 tggtccgcgc ttggtcgaag cgctctttga cgttacgtag attgaacacg acataatcgg
     8581 cggtggactc gccgatgacc ctaatgatct ggcctttttt gacttcaaaa gtttggccgc
     8641 tgttgctctt gacgattgtt tcgctgatga ttttcatcgc ttcctcccga atatctacat
     8701 tcttgccacg ttagctgatc gagcgcggca aacgcaagaa tacaggtgcg aaaagccgca
     8761 aagattgacc ccgcttgcgg tgagacatat aagcaagagt caattatctt aaggagcttt
     8821 caatgatcaa cggtttcaaa gtctacgatg ccgatgcgca tgcgatcatg agcccggcaa
     8881 tgtggcgtga cctgccggaa gatttcatcc ggcgccggcc tcgagcggtg cggatcatcg
     8941 acgataaaga tctggggcgc tggaacacgg gctggctgat
//
LOCUS       DASUVS010000008         9031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_2653, whole genome shotgun sequence.
ACCESSION   DASUVS010000008 DASUVS010000000
VERSION     DASUVS010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9031
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2653"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VF182_00270"
     CDS             complement(<1..749)
                     /locus_tag="VF182_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX6435535.1"
                     /translation="MKSHDNPLGSNAAESDSIILCSTSQGYSVRATPLRITRDVVVFE
                     VYNPYSILQLSEVLATFRIIVNDQPIYSGRATVSNLVNTGIMLVCEASLEEGWSDIDL
                     LSLVSQPSKLRADFRSFLQQCERINSIQPQYKVVVADMQNYFTELKRWLEHVELGIRS
                     LPSVDRSKTESSVINELAPNLLPSIDSLFGRFEETAADVPIQLLPSHRNYAKRQLHPH
                     LLCSPFAYRTYHKPLGYAGDYEMVNMILRDP"
     gene            complement(901..976)
                     /locus_tag="VF182_00275"
     tRNA            complement(901..976)
                     /locus_tag="VF182_00275"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(941..943),aa:Thr,seq:cgt)
     gene            complement(977..1282)
                     /locus_tag="VF182_00280"
     CDS             complement(977..1282)
                     /locus_tag="VF182_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435536.1"
                     /translation="MKETAQQVLNYLGGSPLVTLVVALVAGWTASKTVASEWRSGFIL
                     WLFVGVAGLFLSQFVLLFSGLQDYIEGISEFRLLFDLIAAYFASFLIAALIHFVRPL"
     gene            complement(1279..1908)
                     /gene="thiE"
                     /locus_tag="VF182_00285"
     CDS             complement(1279..1908)
                     /gene="thiE"
                     /locus_tag="VF182_00285"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX6435537.1"
                     /translation="MPKPGFNLYLITDRTQTKGRDLLWLIEQALDGGVKAIQLREKDL
                     AGRDLFLLAEKARNLCQNYGSSLFVNDRLDIALAVSATGVQLGNASVPIALARQLLGA
                     QKMIGVSTHSLSEALEAQEQGADFVLFGPVYFTPSKAGYGEPQGLNKLKEIVEKISIP
                     VYAVGGIKLANILDIRSTGVSGIALISGIMSSDDPKRATSELLRMIGSA"
     gene            complement(1919..2692)
                     /locus_tag="VF182_00290"
     CDS             complement(1919..2692)
                     /locus_tag="VF182_00290"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX6435538.1"
                     /translation="MQDVFNLGEKVYQSRLIVGTGKYKNFEETKKAIEVSGAEIVTVA
                     VRRVNITDPNKENLLDYLSPKKYTILPNTAGCYTAEDAVRTCRLAREAGVGKMVKLEV
                     IGDDKTLYPDVPATLEAAKILVKEGFIVLPYITDDPITAQRLQDIGCAAVMPLAAPIG
                     SGLGIRNPYNIRIILEQASVPVIVDAGVGTASDAAIAMELGCHGVLMNTAIAGARDPI
                     LMAEAMRLGIEAGRKAYLAGRIGKKLYATASSPLEGVLA"
     gene            complement(2697..2915)
                     /gene="thiS"
                     /locus_tag="VF182_00295"
     CDS             complement(2697..2915)
                     /gene="thiS"
                     /locus_tag="VF182_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX6435539.1"
                     /translation="MNTVFFMKIHINGEQKEIAHGLNIAGLLDELQIRPGRVVVELNR
                     TIISREVQESTSLKDGDVLEIVHFVGGG"
     regulatory      complement(2988..3096)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3252..4196)
                     /locus_tag="VF182_00300"
     CDS             complement(3252..4196)
                     /locus_tag="VF182_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX6435540.1"
                     /translation="MRFVTFTRKNEQRIGLIGPQDHIIDLAEVNRRFLKNGNASYLTS
                     MQAFIEAGSRAIQVARQAERYVAGKTVADQKKLAGVGALLRPNQARIIAPIPVPKKNV
                     VMLGINYREHVDEGARARSLEIKYPDYPVFFTKPSTSVIGQFGKVINHKATEKLDWEV
                     ELAVVIGRRGRDIPKEKAYDYVFGYTVCLDMTARELQRQHGQWFKGKSLDTFCPMGPW
                     IVHKSALRDPQQVQLVCRVNGEVMQDGNTRDMIFDIPTTIESLSQGMTLEPGDMISTG
                     TPSGVGFARVPPVFLKPGDKVEGEIEGIGVLEVQIVAP"
     gene            4933..6300
                     /gene="holA"
                     /locus_tag="VF182_00305"
     CDS             4933..6300
                     /gene="holA"
                     /locus_tag="VF182_00305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX6435541.1"
                     /translation="MKGNFDTFLTDVQKGDGPALVLLFGDDLEVQSACKSLIDVVVPP
                     DQREFNLERFDGRSALWDQIESSLLTPPFLPGKKLVWIENAPYFFSRENKTELGKRII
                     QLWRDDKKDDAGKLLVDLIALEGWSQKQWEQLEPSRDTAVFELLQAKDQDDKEATDAL
                     VAYCKSRGLELKEARESEENAIRKLLNGGLPSWDFLLLTTSQVDRRTRLYKQFEENGA
                     IFFLGLERDRYGKISRDKLREFIDRQLSLSKKRVEPQARELILLRAGDDVRTLQQELD
                     KLLLYIGDDTVIRADNVETIFADQSEGWIFDLTRFIAVRNATAALCQLNRLIAQGDHP
                     LKLLSTLAAEVRKLLSARQILDSDLRGRWKPGMNYKQFEHAIVDPGATLLTRSPYADY
                     LCFQRAETFSFAELLSYLHSIHDTDLRLKSSGANPKLVMERLIFQMCLGAPAVPSDAG
                     RRLAL"
     gene            6297..6833
                     /locus_tag="VF182_00310"
     CDS             6297..6833
                     /locus_tag="VF182_00310"
                     /inference="COORDINATES: protein motif:HMM:NF019509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEX6435542.1"
                     /translation="MKPVLLLIAGVILFTPIASANGAMAVQTCFSPQGKCAGHIVREI
                     ENAKRELLVAVYAFTSDELAGALTKAKKRGILVQVIIDRDFDSRNYNSKGKFLEAQRI
                     PVRRLPGSQAANSTREPGLMHQKFAVVDRQIVLTGSYNWTQSADSINDENLLFFRDAG
                     PLAEEYRRIFLRLWERNS"
     gene            6830..7357
                     /locus_tag="VF182_00315"
     CDS             6830..7357
                     /locus_tag="VF182_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435543.1"
                     /translation="MKEFSSKQAISLIAAFVAGFFTLFLYQRWNPLVGKVDEATTMDV
                     AALQSSSDLPSFAVTFVHLPAIEQPAEPPLLQALEVEKIRSLTGKPARVRGRIFRVGH
                     SVKSNTYFLNFGPSQSALTAVIFAPAADLFEQAKLPPKGFDGKAVEVRGQIKDHPQYG
                     LEIVLDDPSQIRILK"
     gene            7589..8824
                     /locus_tag="VF182_00320"
     CDS             7589..8824
                     /locus_tag="VF182_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEX6435544.1"
                     /translation="MTATGLLIAYALLLNHAVWAFSPDDDLSARALLLMDAKTGKILY
                     QKNAHVPLPPASTTKVLTAVLALESQKRSSDRLIVSKAATRVPASKLYLKPGQTVSIE
                     DLLYGVLLASANDASVVLAEGIGGSVERFAEIMTKKAHDLGAINTHFSNPHGLTAPDH
                     YSTAHDLALIFRYAMKNPTFRQIVHTKMSSVRSLSPGKKSARTRLISVRNHNRLLWNY
                     DGALGGKTGYTFAAQKCYVGAVERNGVTLIVSLLGSRDLWGDTKRLLEYGFDNYQALN
                     TLTPAAVTRSITAERVTRDTAKLSALVVMPPEETAPKSSTESYVVQVGSFREQLRAEG
                     LLRAFAAKGFEAFVEKTAVSDRGETSYRVRLGPYAELSEAEQIRQELLKEGGHSAIIL
                     PYSIISEDEAVGGRIEPPL"
BASE COUNT         2226 a         2331 c         2288 g         2186 t
ORIGIN      
        1 tttggatctc tcaggatcat gttcaccatc tcataatcgc cggcataacc taagggcttg
       61 tggtaggtgc ggtaggcgaa gggggagcat agtagatggg gatgaagctg ccgcttagca
      121 tagtttctat ggctcggtaa aagttgtatc ggaacgtcag cggcggtttc ttcaaaacgt
      181 ccgaatagtg agtcgatgga gggaagaagg ttgggcgcca attcgttaat caccgaagat
      241 tcggttttcg aacggtcaac cgacggcaac gatcggatac ctaattcaac gtgctcgagc
      301 cagcgtttga gctctgtgaa atagttctgc atatccgcga ccaccacttt gtattgcggt
      361 tgaatgctgt taattctctc gcattgttgg agaaaactcc ggaagtcagc gcgcaatttg
      421 ctcggctgag ataccaggct gagaagatcg atgtctgacc agccttcttc gaggctagcc
      481 tcgcagacaa gcatgatacc tgtattaacg aggttactga cggttgcccg acccgaataa
      541 atcggttgat cattaacaat gattctaaag gtcgctaaaa cctcggagag ctgaagaatg
      601 ctgtaaggat tgtagacttc gaaaaccact acgtcgcgtg ttatgcggag cggggtcgct
      661 ctgacgctat agccctggct ggtcgaacag aggatgatgc tgtcgctctc ggctgcatta
      721 gacccgagcg ggttgtcgtg actcttcata gacagagcgg cactcgcgtc aaaagaatcg
      781 attcctttta ttgctacgct tacttttcgt caatagcatc ggaatcgcag ttccggcaaa
      841 tttaagatag ccaagcggcg gaagctcgat ggaatcaaag cgcagattcc gccgcgaaac
      901 tggagccacc cgccggagtc gaaccggcga cctactgatt acgaatcagt tgctctacca
      961 actgagctag ggtggcctag aggggtctta cgaaatggat cagagccgcg attaagaatg
     1021 atgcgaaata ggcggcgatt agatcaaaca agagtcggaa ttcggagatg ccctcaatat
     1081 aatcctgcag accggaaaaa agcaacacaa actggctcag gaataatccg gccacaccta
     1141 cgaacagcca taagataaaa ccagaacgcc actcgcttgc gaccgttttg ctggcggtcc
     1201 agccggctac taatgctacg accaaggtca cgagcggcga tcctccgaga taattcagga
     1261 cttgctgcgc agtctctttc acgcgcttcc gatcattcgc aaaagctcgc ttgtcgctct
     1321 tttcggatca tcggaactca tgatcccgga aatgagcgct atcccggaaa cacccgttga
     1381 tctgatatcg agaatatttg ccaacttaat cccgccgaca gcataaaccg gtattgagat
     1441 tttctccact atttccttca gtttatttaa cccctgaggc tcgccatagc cggctttgga
     1501 cggggtaaaa taaaccggtc cgaataagac gaaatcggcg ccctgctctt gcgcttccaa
     1561 ggcttcgcta agagaatgcg tcgacacgcc gatcattttt tgcgctccca acagttgtcg
     1621 cgcaagcgcg atgggtaccg aggcgtttcc caactgtact ccggttgcgc tcaccgctag
     1681 cgcaatatcg agacgatcat tgacaaacaa cgaggaaccg taattttgac aaagatttct
     1741 cgctttctcg gccaacaaga acagatcccg gccagccaaa tctttttccc ttagttgtat
     1801 ggctttaacg ccgccgtcca gagcctgctc gatgagccag agtaaatcac gccctttggt
     1861 ctgggttcga tcggtgatca gatacaagtt gaaaccgggc ttcggcactt gggctggctc
     1921 acgccaaaac gccctcgagg ggactggatg cggtggcgta gagtttcttg ccgatacggc
     1981 ccgcgagata cgctttgcgt ccggcttcga taccgagccg catcgcttct gccattagga
     2041 tcggatctcg tgcgcccgca atggctgtgt tcatcaaaac gccatggcaa cctaattcca
     2101 tcgcgatcgc cgcgtccgaa gcagttccaa cgcccgcatc aacaatcact gggacactgg
     2161 cttgttcgag aatgatacga atattataag ggttgcggat gcccaagccg gaaccgatgg
     2221 gcgcagccaa cggcatgact gcggcacatc caatgtcttg aagtctctga gccgtgatcg
     2281 gatcgtcggt tatataagga agcacgatga atccttcctt taccagaatc ttggcggctt
     2341 ccagagtagc gggcacgtct ggatacagag ttttatcatc gccgatcact tccagcttga
     2401 ccatctttcc aactcccgct tctcgagcta atcggcatgt gcgaacggca tcttcggcgg
     2461 tgtaacagcc ggcagtgttt gggagaatcg tgtatttttt tggactaaga tagtcgagca
     2521 gattctcttt gttaggatcg gtgatgttca cccgccgtac tgcaactgta acgatctccg
     2581 cgccggagac ttcgatggcc tttttggtct cttcaaagtt tttgtacttc ccggtcccaa
     2641 cgatcagtcg tgactggtaa accttttcac ccaaattaaa tacgtcttgc atagctttag
     2701 cctccaccaa caaagtggac aatctcgagg acgtctccat ctttcaacga tgtcgattcc
     2761 tgcacctcac gtgaaatgat tgtgcgatta agctccacca caacgcggcc aggccgaatt
     2821 tgtaactcgt ccagtagccc cgcaatattg agcccgtggg cgatttcttt ctgttctccg
     2881 ttaatatgga tcttcataaa aaagaccgta ttcacttcga ccaccggaag gtggttggga
     2941 atacggtctt aagtttgtac cgtccggatt tcaagcatcc aaacggaacc atgactttca
     3001 cgcttcccta cgccggcgca aaccggatca ggttcgaagg gttgcctgcg ctggcaggct
     3061 ctcagtccga ttaagcagac acccctagct tgcttgaacg attaacagag cgagaaaacc
     3121 tagtcaactc cttaagacag tcgaagtgtt cttgtaactc aagtttaaag atcaccgatg
     3181 cccggccctg ctaagggatg ttcaatctgg agcaatagct ttccttgagc taagcagcgg
     3241 atatgcccca cttagggcgc tacgatctgg acttctagaa ctcctatacc ttcgatctct
     3301 ccctcaacct tatctcccgg ctttagaaac actggcggaa cgcgagcaaa tcctacaccc
     3361 gaaggagttc ctgtgctgat catgtctccc ggctccagcg tcataccctg cgacagcgat
     3421 tctatggttg tgggtatatc gaagatcatg tcacgtgtgt tgccgtcttg cattacttca
     3481 ccattgacac ggcagacaag ctgcacttgc tgcggatccc gaagggcgct cttatgaacg
     3541 atccagggtc ccatcggaca aaaagtatcc aacgatttac ccttgaacca ctgcccatgc
     3601 tgacgttgta gttcacgtgc ggtcatatcg agacatacgg tataaccgaa aacatagtcg
     3661 taagctttct ctttagggat atcccggccc cgcctgccga tcacgacggc cagttcgact
     3721 tcccaatcga gtttttcagt ggctttatga ttgattacct tgccgaactg tccgatgacg
     3781 ctagtcgatg gcttagtgaa gaagaccggg taatctggat attttatttc aagcgaccgc
     3841 gctctcgcgc cttcgtcaac gtgttcccgg taattgatgc cgagcatgac cacgtttttc
     3901 ttcggcaccg ggatgggcgc aataattcta gcctgattag gccgtagtaa cgctccgaca
     3961 ccagcgagtt tcttttgatc cgcgacggtt ttgccggcaa cgtatctttc ggcctgacgc
     4021 gcaacctgaa ttgctctaga gccggcttcg atgaaagctt gcatgcttgt cagataggac
     4081 gcattgccgt ttttcaaaaa ccggcgattg acttccgcga gatcgatgat gtgatcttgc
     4141 ggtcctatta gcccaatccg ttgttcattc tttctcgtaa aagtcacaaa gcgcatagtt
     4201 gcctccgagt gaatcgtttt ggaaagtttt tagctgaagg tggagaagct agactgggtt
     4261 gcactgtttc catgttccat tgtcgcagga ttcgatgttg ctcctctgga cggagttaga
     4321 ggatcggaga atccatttcg ctccgaaagc gttctgccgt gaggcacgct ctatcaaagt
     4381 tgctgactca atactccagg ggctctctcg tctaagtgat ggcattgaac caggactttg
     4441 acgtatcgat ggcgaaacca aatcgaacca gctataggag aggggctgaa gattgtcaag
     4501 caagcgttgt ctgtgacaaa tcccagctaa tccgccccta gggggctcta ggcgtatcaa
     4561 ttctgaaaaa tttgcctgaa cactccggca gtgatatttt tcgatcatga aaggtcgcag
     4621 agatagccgg ccgacaatgc gcgtcggtga ccgtattttg atcaacaagg gaatctcaaa
     4681 gaaagtactc attatcgccc gagttgagat tcctcattcc aacgaaagaa ttttcgtttc
     4741 taaatctggc gggaaataca caatgggtct caaggtgtgg aagtcagatt atgcttggag
     4801 tgagaaaaac cagatgtggg ttcccaagca ttaacctact tcctatagat ttctccaatc
     4861 tggccgtaat ttagatcccg cgtaacgcca aaattgagcg ggcgagaagc tttagctcaa
     4921 gcaggctata caatgaaagg caatttcgac acttttctga ccgatgtcca aaagggtgat
     4981 ggaccggcct tggtgcttct cttcggcgat gacttggagg tacaatccgc ctgcaagtcg
     5041 ctgatcgatg ttgtcgttcc cccggaccaa agagaattca atctcgagcg gttcgatggc
     5101 cgctcagcgt tatgggatca gattgaatcc tcgcttttga ctcccccgtt cttgcctgga
     5161 aagaagttag tttggataga aaatgcgccg tacttttttt cccgcgagaa caaaaccgaa
     5221 ctcggcaaaa gaataattca gctgtggagg gatgacaaaa aagatgacgc aggcaagcta
     5281 ttagttgatc ttatagcgct cgagggctgg agccaaaaac aatgggaaca gctcgaaccg
     5341 tccagggaca cagcggtatt tgagctgtta caggcaaagg atcaggacga taaggaagca
     5401 accgacgcgt tggtcgcata ctgtaagagc agaggacttg agcttaaaga agcccgtgaa
     5461 tccgaagaaa acgcaattcg aaaactattg aacggaggtt tgccgtcctg ggattttttg
     5521 ctcctcacta catcccaagt ggaccggcga acgcgacttt acaagcaatt tgaagaaaac
     5581 ggcgcaatat tctttcttgg tctggagcgt gatcgttatg gcaagatcag ccgagacaag
     5641 ctgcgggagt tcattgaccg gcaattgagc ttatctaaga agagggttga accacaagcc
     5701 cgcgagttga tcttacttcg tgccggggat gatgtacgca cgctccagca ggagctcgac
     5761 aaattgttgc tctacatcgg cgatgatacc gttattcgcg ccgataacgt tgagacaatc
     5821 ttcgcagatc aaagtgaggg ttggatcttc gatctgacca ggtttattgc cgtgcgcaac
     5881 gctacagcgg ctctctgcca gctaaatcgg ctgatcgcgc aaggtgatca cccgctcaaa
     5941 ctcctgagca ccctcgcggc tgaagtgcgc aagcttttat ctgcgcgaca aatactcgat
     6001 agtgatttgc gcggccggtg gaagccgggt atgaactaca agcaatttga gcacgcgatc
     6061 gtcgatccag gggcgacatt actgacgcgc agtccctatg cagattactt atgctttcaa
     6121 cgagctgaaa cattctcatt tgcagagttg ctttcatacc tccacagtat tcacgatacg
     6181 gatctacgct tgaaatcgag cggagctaac ccgaaactcg tcatggaacg attgattttt
     6241 cagatgtgtt tgggagcgcc ggcggtgcca tccgatgccg ggcgcaggct ggcgctatga
     6301 agccggttct cctgttgatt gccggtgtga ttctctttac gccaattgcc tctgccaacg
     6361 gcgccatggc tgttcaaact tgtttcagtc cacaaggcaa gtgcgctggc catattgtac
     6421 gggaaattga gaatgcgaag agggaacttc tggtagccgt ttacgccttc actagcgacg
     6481 agttagccgg cgcgctgaca aaggccaaga agcgaggcat tctggttcag gtcatcatcg
     6541 atcgagactt cgactcgcgc aattacaact cgaaaggcaa atttctagaa gcccaaagaa
     6601 ttccggtacg ccgtcttccc ggcagccagg ccgccaattc gacacgagaa cctgggctga
     6661 tgcaccagaa attcgccgtg gtagaccggc aaatcgtatt aacagggtct tataattgga
     6721 cccaatccgc cgacagcatc aacgatgaga atcttctgtt ttttcgtgat gctgggcccc
     6781 tcgcagagga gtatcggcgg atttttctcc gcttgtggga gagaaattct tgaaagaatt
     6841 ctcctcaaaa caagcaattt cactgatagc agcatttgtc gcgggcttct tcaccctatt
     6901 tctctatcaa cgctggaatc ccctggttgg aaaagtggac gaagccacga cgatggatgt
     6961 ggcggcgctt caatcgtcct ctgatcttcc aagtttcgcc gtcacgttcg tgcatttgcc
     7021 cgctattgaa cagcccgccg agccgcctct gttacaagcg ctcgaggttg aaaagatccg
     7081 tagcctaact gggaaacctg cgcgggttcg cggccgcatt tttcgagtcg gccattcagt
     7141 caaaagcaac acttactttc tcaatttcgg gccttcccag tccgcactaa ccgcagtcat
     7201 atttgcgccg gccgcggatc tcttcgagca agctaagctg ccgccgaaag ggtttgatgg
     7261 gaaggctgtg gaggtgcgtg gtcagataaa agatcatccg caatacgggt tggaaatcgt
     7321 tttggatgat ccgtcacaga tcaggattct aaaataatcg cgaatcgtaa ccggctaaat
     7381 ggcacatcga cgctgtcggg gcctctcagg cagctcccca aaccgtacgt cgcgttcaac
     7441 tattcatgaa cagtagacta atgggtaaat ttccagtgac aaagagctac cccaacgtcg
     7501 ccaaccgtga tataagacgg ttttatttag cgctcagtga agaagccgcg aaagagaggc
     7561 caagaagctg aaaaaattca acttacatat gaccgccacg ggtttgctaa tcgcctacgc
     7621 actactgctc aatcatgccg tctgggcgtt ttctccggac gacgatttat ccgcacgcgc
     7681 attgcttttg atggacgcga aaacagggaa aatcctctat cagaaaaatg cccatgttcc
     7741 gctgccacca gcgagcacga ccaaggttct tactgcagtt ctagcccttg agagtcagaa
     7801 acgatccagt gatcgactaa tcgtctccaa agctgccacc cgggtgcctg cctccaagct
     7861 atacctgaag ccgggacaaa cagtcagtat cgaagacctg ctttatggtg tgctgctggc
     7921 ttccgcgaat gacgccagtg tcgtattggc cgaggggatc ggaggatcgg ttgaaagatt
     7981 cgccgaaatc atgacgaaaa aggcacacga cctcggtgcg atcaatacgc actttagtaa
     8041 tccgcatgga ctgaccgcgc ccgaccatta ctccaccgct catgatctgg cattgatttt
     8101 cagatatgcg atgaaaaatc cgacttttcg ccaaattgtc cataccaaga tgagttctgt
     8161 gagatcgttg tcaccgggaa agaagagcgc ccggacccgg cttatttccg tgcgcaatca
     8221 caaccgtctt ctgtggaact acgatggcgc cctaggcggc aaaacagggt atacgtttgc
     8281 cgctcaaaaa tgctatgtcg gcgcggttga gcgaaatggt gtaaccctga tcgtctcatt
     8341 actcggctct cgcgatcttt ggggcgatac caaacgcctt ctcgaatacg gcttcgacaa
     8401 ttatcaggcg ctcaatactc tgacgcctgc cgcagttacg cgatccataa ctgccgagcg
     8461 agtcactcgg gacacggcaa agttatcggc cctggtcgta atgccgccgg aggagacagc
     8521 gcccaaatcg tctaccgaga gctatgttgt gcaagtcggc tcatttcgcg agcaactcag
     8581 agcagaaggt ctgctgaggg cgttcgcagc aaaaggtttc gaggcttttg tcgaaaaaac
     8641 agctgtaagc gacagaggtg agacctctta tcgtgtccga ttgggtcctt atgccgaact
     8701 gtcggaagcg gagcagatcc gtcaagaact gttaaaagaa ggcggccatt cggccatcat
     8761 cctgccctac tcgatcatct cggaagacga ggccgtcggc ggccggatag agccgccgct
     8821 gtagtttata cctccacaat cattccatct tctgccattg cgcaggagat ttcggctcgc
     8881 cgagcaagca tgtcgcggcc catgtgagtc agtatgattc gagcgcagct taatcttggc
     8941 agttgttctc gcagctgcaa gtaattaatg tggttcgagg tttcccgatc gtaataggta
     9001 cactcacaga taaaaacatc gaccgcgtgg g
//
LOCUS       DASUVS010000009        15560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_876463, whole genome shotgun sequence.
ACCESSION   DASUVS010000009 DASUVS010000000
VERSION     DASUVS010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 15560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15560
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876463"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /gene="phoU"
                     /locus_tag="VF182_00325"
     CDS             <1..610
                     /gene="phoU"
                     /locus_tag="VF182_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX6435545.1"
                     /translation="LVEDQIQRSIKSLVDRNSKLAEVIIERDHEVNRLDVGIDELCIR
                     LLALHQPAGRDLRFITTGLKITTDLERIGDMAVNICERALELNQEPQLKPYIDIPRMA
                     QIAQRMIQQSLDAFVREDTDLALRVCKDDSEVDQLNAQIFREIITFMIENPQTISRAT
                     KISSVSKYLERIADHATNIAEMVVFMVKGKSIRHMKELPPSV"
     gene            613..1335
                     /locus_tag="VF182_00330"
     CDS             613..1335
                     /locus_tag="VF182_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6435546.1"
                     /translation="MSATGQTILVVEDEPDIRKLINYNLAQDRFKVLEAEDGEQALKV
                     LQQETPNLVILDLMLPGLSGLELCKILRDRQDTSRLPILMLTAKTGEADKVVGLEMGA
                     DDYLSKPFSPREMVARVRAILRRTDSSASPPEITPFYHKGALKIDFTTYEVFVRAQQV
                     KLTLKEFELLRFLVHNPNRVLSRDQLLDRVWGGETFVTPRTVDVYIRRLRKAVEKDDR
                     EPKWILTLRGVGYKFDEKALAE"
     gene            1313..3118
                     /locus_tag="VF182_00335"
     CDS             1313..3118
                     /locus_tag="VF182_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6435547.1"
                     /translation="MKRLWQSSLIFKFFLSYLALITLLFSSFYFYSSTIIRRAYVSSL
                     SDRLEREARLLATTTPWSFDNQALDLLSRKLARQLGARITIIGSDGRVHGDSGEPSSG
                     MENHASRPEVVEALATGTGESVRYSTTTGNDLIYRAVLQSQGSDQRIIRLAIPLSDIE
                     EEIDSLRLTLLVAFVGFSSLGLLLSYGFSRQLSARIKRLADFARQVAQGIFPRNFFAK
                     STHDELDALERHLSEMSESIRENVEQVVAEKEKASSILRCMMEGVLVLDPKGHVVVIN
                     QQAERMFHLPEDGDSRGALILEISRHPEMRNILRDVLAFDFTKGPYRKEVELDQGRWF
                     RVNAVSFRMDQSRDSGSVLVFHDITEIKQVEILRADFVANVSHELRTPLTAIRGYAET
                     LRQTPPDDPVDAQHFLSIIEKHAERLSRLTEDLLTLSDLESGKIELDFTPIDIARLVQ
                     SVFEMFSDQANRKQVTLERVLEPGLPPVLGDLDRLQQLFINLVDNAVKYTPINGTVTF
                     KAQRAMIDKSIDGVEVSIADTGTGIPEEAIPRLTERFYRVDKARSRDLGGTGLGLAIV
                     KHIAQAHGARLKIESTLHKGTAISVLLPAVAEASP"
     gene            3518..4189
                     /locus_tag="VF182_00340"
     CDS             3518..4189
                     /locus_tag="VF182_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HEX6435548.1"
                     /translation="MTISEAYCYIESFTSIHFSMFNFAPHKEKFFDLFKQAAENALAG
                     ARALKEMLEHYDNPQDSWKKIKDLEHEGDRITHRTIRSLNQTFLTPIDAEDIHALITA
                     LDNVMDAIEAAASRMILFRIDQPTAEAKELSDIIVTSTEQLVKAVSHMPRLDDIDEYC
                     IEINRLENTADDIYRKAISRLFENGTPPLDVIKWLDVYEILESATDRCEDVANTLETV
                     GLKNS"
     gene            4211..5209
                     /locus_tag="VF182_00345"
     CDS             4211..5209
                     /locus_tag="VF182_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX6435549.1"
                     /translation="MLDGNLLLFLVLGLILAAEFVNGWTDSPNAIATVVSTRVLSPYQ
                     ALIMATVLNAIGAMSGTAVAATIGKDIVRPEIINLTTVAAAMIGIIFWSTLAWYYGLP
                     TSESHALIAGLTGAGLATAGPESLLWTGWSKVLIGLIFSSFLGFGGGLLLMGFLYRAL
                     AHSRPGKVRRIFGRAQIVSAAFMAFSHGSNDGQKFIGVFTLALLLGGILPEFRVPVWV
                     ILLCAVTMGVGTAVGGWRIVRTMGLRLTKLEPVHGFAAETAAAVTIEVATRLGIPVST
                     TQTINTAIIGVGATRRLSAVRWGVTFEIVTAWILTFPVCGMIGWLATKILMFFEAV"
     gene            complement(5233..5526)
                     /gene="bamE"
                     /locus_tag="VF182_00350"
     CDS             complement(5233..5526)
                     /gene="bamE"
                     /locus_tag="VF182_00350"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEX6435550.1"
                     /translation="MGRIIPGIFVSLILSGCFYQGRNFDISPVRNIKNDVTTQREIYA
                     DFGEPLRRGLENGLETWVYGYQYYELGQLRESKDLYVVFNKNNTVRSYSFTSR"
     gene            complement(5534..6154)
                     /gene="recR"
                     /locus_tag="VF182_00355"
     CDS             complement(5534..6154)
                     /gene="recR"
                     /locus_tag="VF182_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX6435551.1"
                     /translation="MSTYPAPLARLVQELSKLPGIGEKTAARLAFHMLKGKKDDVFNL
                     ADSIGKLRKEMGLCRICFGFSEVDPQSGETSVCMICRNPERDQRSICVVEEPSDLIAV
                     ERSQEFRGLYHVLHGTISPLDGIGPDALRIKELLGRIKSEVVQEIIVATNPTMDGEAT
                     ALYLSKVIKPLGVAVTRIARGLPMGGDLEYTDAVTLGKALEGRREI"
     gene            complement(6151..6477)
                     /locus_tag="VF182_00360"
     CDS             complement(6151..6477)
                     /locus_tag="VF182_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX6435552.1"
                     /translation="MAKGIGGMGNLLKQAQEMQSRMAKIQEELAQKTVDGSAGGGMVR
                     VTVNGQLALTAIKIDPTVINSEEKEMLEDLIMAAINDGMRKARDMASSEMSKITGGLK
                     IPGLSV"
     gene            complement(6520..8190)
                     /gene="dnaX"
                     /locus_tag="VF182_00365"
     CDS             complement(6520..8190)
                     /gene="dnaX"
                     /locus_tag="VF182_00365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX6435553.1"
                     /translation="MSYLVLARKWRPQNFEDVSGQSHITRTLQNAIRAGRIAHAYLFT
                     GVRGVGKTTAARILAKALNCAQGPTPTPCNECVQCTEIIQGSCIDVIEIDGASNRGID
                     EVRQIIENVRYRPARCAFKIYIIDEVHQVTKDAFNALLKTLEEPPASVKFILATTEPH
                     RLPETILSRCQRFDFRRISLKEIVQRLSDISAREGLKISQGALIAVAREADGSMRDAQ
                     SLLEQVLACFGADQQTGEVDEVLLQEVLGLAERRMLFDISESVIGGDGKRCLELMAQA
                     AEQGRDFTRLAKDLVEHFRNLMVARLCAQQKQKNLAEEARSELLDLPDQEVVALGSQA
                     AKVSVETLLDYLELMSAGEDEVARAANPRFLLETLLMRLTMLTKTLPISQLIERLTVL
                     EKRLVSEAQGTPAEMKTGVPRISSMVDSNPSEATALPAASLVIPEDKEQFWHAFVSFV
                     AKEKKLLASHLESGCALAIAPESLTIGIAEKHHLSYLQDLENMTLLRSFAKRFFSREV
                     TVQLTQLSAAPAKSQQESFASAETGSEMVKEALRIFGGSIKGVRTDNS"
     gene            complement(8298..8395)
                     /gene="ffs"
                     /locus_tag="VF182_00370"
     ncRNA           complement(8298..8395)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VF182_00370"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8483..8573)
                     /locus_tag="VF182_00375"
     tRNA            complement(8483..8573)
                     /locus_tag="VF182_00375"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8537..8539),aa:Ser,seq:gga)
     gene            8990..9151
                     /locus_tag="VF182_00380"
     CDS             8990..9151
                     /locus_tag="VF182_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435554.1"
                     /translation="MNIIVGHCVGTVRYGYSLLSLEQEEDCLFVPPLQLAMVGKREYL
                     PALDVDRNS"
     gene            complement(9228..9599)
                     /locus_tag="VF182_00385"
     CDS             complement(9228..9599)
                     /locus_tag="VF182_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6435555.1"
                     /translation="MFKPMTKILVVENHADMREILVRILELMGFTAITSKNGREGVEK
                     ALAEKPDLIVMDIMMPEISGWEATRILRSNPETKDVPVLAATVLFRPTDLQRCINVGC
                     NDYIVKPFTVKQLRRKIERLI"
     gene            10267..10785
                     /gene="tadA"
                     /locus_tag="VF182_00390"
     CDS             10267..10785
                     /gene="tadA"
                     /locus_tag="VF182_00390"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX6435556.1"
                     /translation="MPQILTTTFSSDASLMKLALQQARDASERGEVPVGAIIVHNDQI
                     IGRGSNRREELQSPIAHAEIIALDEASRTLGYWRLTECDLYVTLEPCIMCAGAILQGR
                     LRRLIFGCLDPKAGAVMSLFNLCDDRRLNHRLTVTGGVLAEECANVLSEFFNLVREQK
                     RSTPCGEVAEPG"
     gene            10762..10853
                     /locus_tag="VF182_00395"
     tRNA            10762..10853
                     /locus_tag="VF182_00395"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10798..10800,aa:Ser,seq:cga)
     gene            11558..11935
                     /locus_tag="VF182_00400"
     CDS             11558..11935
                     /locus_tag="VF182_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6435557.1"
                     /translation="MKILVVEDNAAVRDLLARILERIGYVPVLASHGKEGLAKAISER
                     PNLIIMDMRMPIMDGWATARALRANPDTKNIPILATTALSQSHQLKTCLDAGCNGYIV
                     KPFSVRTLQRKIKKLLAAPIAEV"
     gene            12465..12641
                     /locus_tag="VF182_00405"
     CDS             12465..12641
                     /locus_tag="VF182_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435558.1"
                     /translation="MTPEDKLRLEELGKFGISIADREWIADIIRRQDEEIERLKIRLN
                     MLLPKIRGQGGRVA"
     gene            complement(13685..13840)
                     /locus_tag="VF182_00410"
     CDS             complement(13685..13840)
                     /locus_tag="VF182_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435559.1"
                     /translation="MISHSTVTYACHIGARALLPHSACESTASFGPDIAAQPGGGFDV
                     IAIIESF"
     gene            14202..14345
                     /locus_tag="VF182_00415"
     CDS             14202..14345
                     /locus_tag="VF182_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435560.1"
                     /translation="MVETVLTGTARAPLQPAKLFNQLTMVQQFDLFAVNRENAEIEPS
                     KLL"
BASE COUNT         3687 a         4097 c         3861 g         3915 t
ORIGIN      
        1 cttagtggag gatcagattc aaaggtcaat caaatctctg gttgaccgga attccaaatt
       61 agccgaggtg attatcgagc gagatcatga agtgaatcgc ctcgatgttg gcattgatga
      121 actctgcatc cgcttgctcg ctcttcatca gccggccgga cgagaccttc gtttcatcac
      181 caccgggctg aagatcacca cggacttgga gcggatcggc gacatggcgg ttaatatctg
      241 cgagcgagcg ttagagctca atcaggagcc gcagctgaag ccgtatatcg atataccgcg
      301 catggcgcag attgcccagc gtatgatcca gcaaagtctt gacgcttttg ttcgcgagga
      361 caccgatctc gcgctccggg tttgtaagga cgacagcgaa gtcgatcagt taaatgcgca
      421 gatattccga gaaatcatca cctttatgat cgagaatccc cagaccatta gcagagcgac
      481 aaagatcagc tcagtttcaa aatatctcga acgcatcgcc gatcacgcca ctaacattgc
      541 cgagatggtt gtgttcatgg tgaagggcaa gagcatccgt cacatgaagg aattaccgcc
      601 ttcggtttga taatgagcgc caccggtcaa accattctcg ttgtcgaaga cgaaccggat
      661 attcgcaagc tgataaacta caacctggcg caagaccggt ttaaggtgct cgaggcggag
      721 gatggagaac aagcgctgaa agttctacag caggaaacac caaatctggt gatccttgat
      781 ctgatgttac ctgggctctc cggtctggag ctatgcaaaa tcttgcgcga cagacaggat
      841 acatcccgcc tgccgattct tatgctcacc gcgaaaaccg gagaggctga taaggttgtc
      901 ggtttggaaa tgggcgcgga cgactacctg tcaaaaccgt ttagcccgcg tgaaatggta
      961 gcgcgggttc gagccatcct gcgccgcacg gactcttccg cctcaccccc ggaaatcaca
     1021 ccgttttatc ataagggcgc attgaagatc gactttacga cttatgaggt tttcgttcga
     1081 gcccaacaag ttaaattgac gcttaaggag ttcgagcttc tacggttttt agtccacaat
     1141 ccgaatcggg tgctaagccg cgatcaattg cttgatcgag tttggggcgg tgaaacattc
     1201 gtcaccccta gaaccgtcga tgtctatatt cgtcgattgc gtaaagcggt ggagaaagat
     1261 gatcgtgaac ccaagtggat tttgactctg cgaggggtgg gctataaatt cgatgaaaag
     1321 gctttggcag agtagcctta tcttcaaatt ttttctcagt tacctagccc tcataaccct
     1381 gctgtttagc agcttctact tttattccag cacgattatc agaagagcgt acgtttcctc
     1441 ccttagcgac aggttggagc gtgaagctcg tctgctcgcg acgactacgc cctggtcatt
     1501 cgacaaccaa gcactcgacc tcctctcacg aaagttagct cgccaattag gcgctcgcat
     1561 caccattatc ggcagcgatg gacgagtcca cggagattcg ggggaaccat cgtccggcat
     1621 ggagaatcac gcttcgaggc cggaagttgt cgaagctctc gcgaccggga ctggcgagtc
     1681 ggtgcgttac agcacaacaa ccgggaatga tttgatatac cgggccgtcc tacaatctca
     1741 gggtagcgat caaaggatta tccggctcgc cattccgctc agcgatatcg aggaagagat
     1801 agattctttg cgcctgaccc tgcttgtcgc ctttgtcgga ttctcgagct tgggcttgct
     1861 tttgtcctat ggattctcac gacagttgag tgcgcgtatc aaacgcctgg cggattttgc
     1921 gcgacaagtc gcgcagggaa tttttccgcg aaactttttt gccaaatcca cgcacgacga
     1981 gcttgacgca ttggagcgcc acctgagcga aatgagcgaa agcattcgcg aaaacgttga
     2041 gcaggttgtc gcggaaaaag aaaaggcaag ctcgatcttg cgctgcatga tggagggggt
     2101 tctcgttctt gatcccaagg gacacgtggt ggttatcaat cagcaggcgg agcgaatgtt
     2161 tcacctgccc gaagacggtg atagtcgcgg cgcattgatt ttggagatat cgcgccaccc
     2221 tgagatgaga aacatccttc gcgacgtact cgcttttgat ttcaccaaag gaccctaccg
     2281 caaagaagtg gaattggatc aaggcagatg gtttcgtgtc aacgccgtga gttttaggat
     2341 ggatcagagc cgcgactctg gctccgtact ggtattccat gacatcactg aaatcaaaca
     2401 agtcgagatc ctgcgcgccg atttcgtcgc caatgtttca cacgaactgc gcacgccgct
     2461 gacagcgatt cgcggctatg ctgagactct tcgtcagact ccgccggacg atcctgtcga
     2521 cgcacaacac tttctgagta tcatcgaaaa gcacgccgaa cgccttagtc gtctgacgga
     2581 agacttactg acgctctcgg acctcgaatc gggcaaaata gaattggact tcactccgat
     2641 cgatatcgca cgtttggtac aaagcgtttt cgaaatgttt tcggaccaag ccaataggaa
     2701 gcaggttaca ttagagcggg tcctcgaacc gggacttccg cccgtgctcg gcgacttgga
     2761 ccgtttgcag caacttttta tcaatctcgt agacaatgca gtgaagtata cgccgatcaa
     2821 cggtacagtg acattcaaag ctcaacgagc catgatcgac aagagtatcg atggcgttga
     2881 agtttcgatt gccgatacgg gtaccggaat acccgaggag gctattccgc ggcttacgga
     2941 aagattttac cgcgtcgata aagcgcgatc ccgggatttg ggcggcaccg gactaggcct
     3001 cgccattgtc aaacacattg cccaggcgca tggagcacgc ctgaagatcg aaagcacact
     3061 tcacaagggc acggcgattt ccgtgttgct gcccgcggtc gcggaagcat cgccatagag
     3121 gttattctcc tctgcacagg caactcgtgt cgccgccaaa tcaactagac tccgctcgct
     3181 cgctgccacc aacggctcac gcatctgacc atcggtactc acatgaacca ctttaatacg
     3241 ctgatactaa cagtgaaaac gaagtaggaa tcgacatttc ccatcaatac tccagcgcat
     3301 cgactaagta tcgatcgacg aagtgattta tatcctgcga tagtcatgcg gagtgtgcga
     3361 tggtgcaacg gaggctgaac gggaaatcgt ccgctgagtg gtccaacatt ggctctagct
     3421 aatgatgaac aggttcttcc atattcggca cgctgaggac gacattcgct gacctgttgc
     3481 tcaaaccact aacgcaaggc aaactgcaga actaatcttg acaatcagtg aagcctactg
     3541 ctacatagaa agcttcacca gcatccattt ctctatgttc aactttgctc cccacaaaga
     3601 aaagtttttt gatcttttca agcaagccgc ggaaaacgcc ctcgctgggg cgcgagcgct
     3661 caaagagatg cttgaacatt acgataatcc acaagactct tggaaaaaaa tcaaagactt
     3721 ggagcatgaa ggagaccgga ttacacatcg gacaatccgc agcttaaacc aaacttttct
     3781 cactccgatc gatgcagaag acatccatgc gctgattacc gctttggata acgtaatgga
     3841 cgctatcgaa gctgctgcct cgcgcatgat cctttttcgt attgaccagc ctactgccga
     3901 agctaaagaa ctgagcgaca tcatcgtgac gtccacggaa caactcgtca aagcggtttc
     3961 gcatatgccg cgattggacg atatcgatga gtactgcatt gagatcaacc ggcttgaaaa
     4021 caccgcagat gacatctatc gcaaagccat tagtcggctc tttgaaaacg gaacacctcc
     4081 tctggatgtc atcaaatggc tcgatgttta cgaaatcctt gaatccgcga cggatcgctg
     4141 cgaagacgtc gcgaacactc tcgagaccgt cggtctgaaa aactcctagc ctcctccggc
     4201 agccctcaac atgctcgacg gcaacctgtt gctctttctg gtcctgggac tcattctggc
     4261 cgccgaattc gtcaacggct ggaccgactc gccgaatgct atcgccacgg ttgtctcgac
     4321 acgagtgctt tcgccttacc aggctctgat tatggcaacg gtgctcaatg ccataggcgc
     4381 catgtcgggc actgcagtcg cggcaaccat tggcaaagat atcgtccgtc cggaaatcat
     4441 caacctcacc actgtggcgg cagctatgat cggaatcatc ttttggagca cgctggcctg
     4501 gtactacggg ttaccgacga gcgagagtca tgctctcatc gccggcttaa ccggcgccgg
     4561 actcgccacc gccggacctg aaagcctgct ctggacgggc tggagcaagg ttctaatcgg
     4621 cctgatattt tcttcctttt tgggttttgg tggcggcctg ttgctgatgg gctttttata
     4681 ccgcgcgttg gctcacagcc ggcccggaaa agtccggcgc atctttggac gagcccaaat
     4741 tgtttcggca gcgttcatgg catttagcca tggttccaac gacgggcaga aattcatcgg
     4801 cgtgtttact ctggctctct tacttggcgg gattttacct gaatttaggg tccccgtttg
     4861 ggtgatctta ctttgtgccg tcaccatggg agtaggtacc gcggttgggg gatggcgcat
     4921 tgttcggact atgggactgc gcctcaccaa gcttgaaccg gttcatgggt tcgcagccga
     4981 aactgccgct gcggtcacca tcgaggtggc gactcggctc gggatcccag tcagtacaac
     5041 tcaaaccatc aacaccgcca tcatcggcgt cggtgccact cggaggcttt cggccgtgcg
     5101 ctggggggtg acgttcgaaa tcgtcaccgc gtggatactg acatttccgg tctgcggtat
     5161 gattggctgg ttggcgacaa aaatattgat gttttttgag gcggtctaat aaccgctccc
     5221 ccacccaaga aactatcgtg aagtgaacga gtaacttctg accgtattat tcttgttgaa
     5281 gaccacgtac agatctttcg actcgcgcag ttgtccgagt tcgtaatatt gatatccgta
     5341 cacccaagtt tccaggccgt tttctaaacc tcttcttaac ggctcaccaa agtctgcata
     5401 aatctccctc tgagtcgtga catcgttttt gatattcctc accggcgaga tatcgaagtt
     5461 gcgaccctga taaaaacatc cggatagtat caacgaaacg aaaatacctg gaatgatgcg
     5521 ccccatgtta cttctatatc tctcgccgac cttcaagagc tttgccaagc gtcaccgcgt
     5581 cggtatactc aagatcgccg cccattggca gcccgcgggc aatccgggtt accgcaacgc
     5641 ccaacggctt gatcactttg gataaataaa gcgcggtcgc ctcgccatcc atcgtcggat
     5701 tagtggcgac gataatttcc tgaaccactt cgctcttgat tcttccgagc aattctttga
     5761 tacgtaacgc atccggtccg ataccgtcca acggggagat ggttccatga agcacgtgat
     5821 agagcccgcg aaattcctgc gatcgttcta ccgcaatcaa atccgacggc tcttcaacga
     5881 cacagatact ccgctggtct cgttccggat ttcggcaaat catgcagacg ctagtttcgc
     5941 cgctttgcgg atcgacctcg ctaaagccga agcaaatccg gcagagcccc atctctttgc
     6001 gcaacttgcc gatactatcg gcaagattga acacatcatc cttctttcct tttaacatgt
     6061 ggaatgccag gcgagccgcc gttttttccc caattccagg aagcttggat agttcttgaa
     6121 ccaagcgggc aaggggcgcg ggataagtgc tcatacggat agtcccggga ttttaagacc
     6181 tcccgtgatc ttactcatct cgctagaagc catatcacgg gccttacgca tgccatcgtt
     6241 aatggcagcc ataataagat cctcgagcat ctctttttct tcggaattga ttacggttgg
     6301 atcgatctta atcgccgtaa gtgcaagctg accattcacg gtcacccgga ccatgccccc
     6361 gccagccgat ccatcgaccg ttttttgtgc cagctcctcc tgaatttttg ccatacgcga
     6421 ctgcatttcc tgggcttgct taagcaaatt acccattccg ccgattcctt tagccatgga
     6481 tcctcctaca aaattggtaa aaacgactct ccccggctgt caactgttat cggtccgaac
     6541 ccctttgatc gagccgccga aaatacgcag ggcttcctta accatttcac tgccggtctc
     6601 agcgcttgcg aaggactcct gctgagactt ggcaggcgcg gcgctcagct gggtcaattg
     6661 gaccgtcacc tcgcgcgaaa agaaacgttt cgcaaaactt ctcaagagag tcatgttctc
     6721 aagatcctgg aggtaactaa gatggtgctt ctcggcaatg cctatggtca aagattcggg
     6781 agcaatggcg agagcgcagc cggactccaa atgggacgcc aatagcttct tctctttggc
     6841 gacaaacgag acaaacgcgt gccagaactg ctctttatcc tcgggaatca caagcgatgc
     6901 agcggggagc gcagtcgctt cgctgggatt tgaatcgacc atactagaga tacgggggac
     6961 tccggtcttc atttccgcag gagtcccctg cgcctcgctg actaagcgtt tctcgagtac
     7021 tgtgagccgt tcgatcagtt gactaatcgg aagagtttta gtcagcatag ttaatcgcat
     7081 tagcagcgtc tctaaaagga aacgaggatt ggcagcgcgt gcaacctcgt cctcccctgc
     7141 tgacatgagt tcgaggtaat ccagtagggt ttcaacagac actttagccg cttgagagcc
     7201 caacgcgacg acctcttgat ccggtaaatc gagaagctct gatcgcgcct cctccgccag
     7261 attcttttgt ttctgttgtg cgcatagccg agcaaccatc aaatttcgaa aatgctccac
     7321 caaatctttt gccaaccgag taaagtctcg tccctgctcg gcagcttgcg ccatcaattc
     7381 cagacatcgt ttcccgtcgc cgccgataac cgattccgag atgtcaaaca gcatgcgcct
     7441 ttcggcgagg cctagaacct cctgcagcag tacctcatcc acttcccctg tttgctgatc
     7501 tgctccaaag caggccaata cttgctcgag cagagactga gcgtctcgca tgcttccgtc
     7561 tgcctcccga gccaccgcga tcaatgcgcc ttggctaatc ttgagacctt ctctggcgga
     7621 aatgtcactt agccgttgaa cgatttcttt tagagatatc cgccgaaaat caaaccgttg
     7681 gcagcgagac aaaattgtct cgggcagccg atgcggctcg gtggtagcca gtataaattt
     7741 taccgaggct ggtggctctt cgagagtttt caagagcgcg ttgaaagcgt ctttagtaac
     7801 ctggtgaacc tcatcgataa tgtaaatttt gaacgcgcag cgtgccggtc gatagcgaac
     7861 gttttcaatg atctggcgaa cctcatcgat cccgcgattg gaggcgccgt cgatctcaat
     7921 aacatcgata cagcttccct gaatgatttc tgtgcattga acgcattcgt tacatggggt
     7981 tggagtcgga ccctgagcgc aattcaatgc cttggcgaga attctagctg cggttgtttt
     8041 gccgacgccg cgaaccccgg taaacaaata agcgtgagct atacggcccg ctcgtatggc
     8101 attttgtagg gtgcgagtga tatgactttg ccccgagaca tcttcaaagt tctgaggacg
     8161 ccacttacgc gcgaggacta gataagacat gtggtttcct tcaagatttt accgcagatc
     8221 gaaagtcaga tcgatcaaca cagacgcgat ctaaggtttt gctaagactt agcgaagacg
     8281 ctgggatgcc cattgctgac agccaggcta ccctgtggca caggggaatt ccggtaccgc
     8341 tgcttccttc cggacctgac ggggttcgcg gctccttctt gcacaggacc taactatcag
     8401 caatcgacat ccgattcgga aaatagcgtc atcctgaacg ctatagctgg tcttctgctt
     8461 ccatcgcgcg catcagctcc tatggcggag agggggggat tcgaaccccc ggtactcttt
     8521 tggagtacac acgatttcca gtcgtgcacc ttcaaccgct cggtcacctc tccttctcgt
     8581 atgcattcat ctagttagga cgagagaaac gcgtgcgcac gcaagaaact accacagtaa
     8641 ggtttaattt tccataaagc taatctgcga gtatcgaaat tgtcacccgg agagactagg
     8701 ttttcatcgg ttcgcggtta ccacgttctt tactcttcga gtaattgagg cctgcagata
     8761 cgctactcag ctggcgcgtg cgcgttgagg aactgcgcct gattatacgc ccggcaaagt
     8821 cctcgcttaa agtatacgac taagaacgcc gagcattttc aaaaaccgac agacgggact
     8881 cacgattgat tccgtcatga ctccaattcg catcttgtct aataacaaat tctcgctgga
     8941 agtatgcttg cgtcactttc tactgaatat cggaactttg gaaatttcca tgaacatcat
     9001 tgtaggccat tgcgtgggga cagtgcgcta tggatatagc ctgttgagtc tggaacagga
     9061 agaggactgc ctctttgtgc cgccgttaca attggccatg gtaggtaagc gcgagtatct
     9121 accggccctg gatgttgatc ggaatagcta acgggctatt ggggcatctg ctgcgattga
     9181 tagtatggta acagctcata cctgggtgag aatcgagagc tgccctgtca gatgaggcgc
     9241 tcaattttcc tacgaagctg tttcaccgta aatggcttga cgatatagtc gttacaccca
     9301 acgttaatgc atctctgaag atcggttggc cggaataaca ccgttgctgc caagaccgga
     9361 acatccttcg tttcgggatt gctgcgcagg attcgagtcg cctcccatcc cgaaatttct
     9421 ggcatcataa tgtccatcac gatcagatcg ggtttctcag ccaaagcctt ctcaacaccc
     9481 tccctgccgt ttttcgaagt gatcgcggtg aaacccatca gttcaagtat tcttacgaga
     9541 atctcacgca tatccgcgtg attttcgaca accagaattt tcgtcattgg cttaaacaaa
     9601 ataaacgcca gtgggtgaat gatgtagggc cggtaatgtc gtacagctcg ggcctctgcc
     9661 cctgcctctc gcactcattc accaaaaagg taaaagtccc ttacattcgc tcgccccagg
     9721 caagagatcc agtcaatgcc ggattataga acatgatgtg cgtgccgcat tgaaatcgat
     9781 tgcttagcct gctgtaaagc tgccgcatca gcagcgttct atattggggg ggctggttca
     9841 ttggatgctt gcgcccgggt ttcttgagct gggtttaggc agttcatgcc tgcaattttg
     9901 gcgcatttcc cgacgacgtt tcttgataca gccacactgc actgatgcta tggcatcttc
     9961 gttactggtg acgccagccg gacaataaca cttaatgatc ccgccgaaga tgatgcaggc
    10021 gaacacatcg cacttccgca ggtattgtgc aaactggcta gtgaccgatt gcgaggaaaa
    10081 aggaccgtga cgccaacttg ggtgcattca cttgctgggt gcagggatca ccagcaacgc
    10141 cgaatgatgt cgtgcaggga cttctgctcg atagcggaca tttattcaag atgatccgac
    10201 ttcaagcata gtactaagtc cacgcaattt attctcgacc atttctgtgc tagcgtttag
    10261 cttctcatgc cgcaaatttt aacgaccact ttttcgagtg acgcttcctt gatgaagctc
    10321 gcattacaac aagcgcgtga tgcaagtgaa agaggcgaag ttcccgttgg cgctattata
    10381 gttcacaatg atcagattat cggcaggggc tccaatcgta gggaagaact gcaaagcccg
    10441 atcgctcatg ctgaaatcat cgccctcgat gaggcttcgc gaacactcgg ttattggcgc
    10501 cttacagagt gtgatcttta tgtcacactg gaaccctgca tcatgtgtgc aggcgcaatt
    10561 ctccagggac ggttgcgccg actcatattc ggttgtttag atcccaaggc cggcgctgta
    10621 atgtcgcttt ttaatctgtg cgatgatcgg cgacttaatc accgattaac agtcacaggt
    10681 ggagtcttag ctgaagagtg tgctaatgtt ctgagtgaat tctttaatct tgtgagagaa
    10741 cagaaaagaa gcacaccatg cggagaggtg gccgagcccg gctgaaggcg cttgactcga
    10801 aatcaagttt acccgaaagg gtaacgtggg ttcaaatccc accctctccg ccagatcatt
    10861 tctcttatcc agcaaagagt gagcccgttt ctcgattgtt ggcaaaacgt ctgtgaaaga
    10921 aaaatcaaat tcccctgtgc ccctcgaaga gtgccctttt gtgtaagaaa tgtgaaaaga
    10981 acttttgaac ccatgttaag ccgtttctcg ctttagcggt acaaccatcc gattctcagc
    11041 ttctttcctg aagtgctctt gaacgcgatt tatcgacccg gtgaggtcgt cattactggt
    11101 tattccgtat gcgataaatc gagtccgttt caggcgctaa gtgcatccct tcgttctcgc
    11161 ttaggcccgc tcgcctgaag tttctaaccg ctgatcggcg cattcgtgag gaactaactc
    11221 cgccatgctg caataattgg atctttgttg ccgggaagcg ccgcgctgaa catttctaag
    11281 aatacgaccg gaggcttgtc aatttgttgg cgtgccgtgt caacgattga ttgctgagac
    11341 ctgtcgtcgg agatgcccat acgtgccctt tgtagaaaat ccatgacatg tccgtccggt
    11401 ctcatttttg cccctcaaat catcgtggag gcaccatgtt aagcgatttg attatacgga
    11461 tggcgacggt gctcgcagtg atatccacaa tggtcattta gcttttcctg tagcttgatt
    11521 gccatgggta aaggatcaga acggataacg gcgagcaatg aagattttgg tcgttgaaga
    11581 taacgcggct gtgcgcgatc tattagctcg catattagaa agaatcggct atgttcccgt
    11641 cttagcgagt cacggtaaag aaggtttagc gaaggctatc tcagagaggc cgaacttaat
    11701 tattatggac atgaggatgc cgataatgga tggctgggca acagcgagag cccttcgcgc
    11761 gaatcccgac acgaagaata ttccgatttt ggcaacgacc gcgctgtctc aatcacatca
    11821 actaaaaacc tgcttggatg cgggctgcaa cggctacatc gtaaagccgt ttagcgttcg
    11881 gactctccag cgcaaaatta aaaaactgct agccgctccc atcgctgaag tatagagtgc
    11941 cttgatcatg cttactgtca tcagaaaatg cccatagaca ccttcataaa ggaatcttag
    12001 gacggatttg gcttagaatc agaattgtgt tatatttgag caacctatgg gtaatttaca
    12061 cgacttcttt gcaagttacg gtaaattcat cttactgatc ggtgcattga aggtcatctt
    12121 tctcgaccct cgccttctac tcactgctct gagtcggaac gttatttcga gtatgtttgc
    12181 ttaacagtgt gccgaagatg tggaacccca tattttcatc ttctcactcg cggttgttag
    12241 gttcgctcgg aagaacaaag cgtctggtcc ataccgtgtc atcgccgtcg agaggcatca
    12301 gccaggagtt cgttccgata ttgtttggga cgcgatggtt gcaccagcat tcgttgttgt
    12361 atccgtggcg gtcttcttca caaaggcgcc taggtccaat agaaactcgg gcaataagct
    12421 gaaggcgcaa aaggctatgc agttcggatt taagcggagg acatatgaca ccggaagata
    12481 aacttcggct tgaggaactg ggaaagtttg gaatcagcat cgcggatcgc gaatggattg
    12541 cggatataat taggcggcag gacgaagaga tagagcggct aaaaataagg cttaatatgc
    12601 tgttgcctaa gattagggga cagggcggca gggtcgcata aagcatataa ttttttgcgc
    12661 taagtcactg ctttcgaatt acctgaaaca ggtctccgcg caaaaaactt ataatctgta
    12721 ttcacgacaa ctgtttttta acggtcctgg cttcgcgttc ggttggattc ggtccaccac
    12781 gcacgaaaaa gcatcagcaa caagaaaagc gcgagtggcg cttaatctct gaggtggtgg
    12841 tagacaatca cgatccgaac ttttttttca gaaaaggtca atcagtagtt gttccttgtt
    12901 ttaggttcaa gctcaccgat cgcagcattc aattacccat agatagagtg atcgaaccaa
    12961 cgcctcatac cgcgctcgcg ctaacgtccg tgtcacaacg atgtgattct acaaacataa
    13021 cgtgctcgat ccggctttct cgcgttccct accgaaattg gtgaatgatc gcgcctcttc
    13081 caggcccgac gcagtaaaac ctcgcctgcg gcaaatctaa ccctatcgcg gccattctcg
    13141 cgcgctttcc gcctgctgcg cgggttggat tcttcccttg tgccgcttcc ctttgcttgc
    13201 gcccaagata gattcctctc acgatcgcca acactcatcg cacatcccga tattgtttcg
    13261 cgtctgccgt ccacattcca cggcagttct tcatagagtg cctcacatat ggctaccact
    13321 tgatcgaccc ggcgtcattg tgctatcaac cgcgccctcg ttgtgcagcg acaacccgcc
    13381 ttcctttggc tacctgtcaa ggcgctgcgc tgacgaggcc cccggaggcg atcgggtaga
    13441 tctacgaggt ttacttattt cgatcgttat gggcttaggt ttttgggctt ttatcttggt
    13501 ctcgttatcc ttgctgatac agtaagccgc gagcccattt tgcattacgg agcaaacatc
    13561 atgatggttc tattgttcaa tttgctcatc gcggcggcag tgactcttgc agctacagga
    13621 gtaatcgctt ttatccttac caacgtcatt ttcggatggt tccaaaagag ccgatcgacc
    13681 atgttcaaaa actctcgatg atggctatta cgtcgaaccc gccgccaggc tgcgcggcaa
    13741 tatcaggccc gaagctcgcc gtgctctcgc acgcgctgtg aggcaagagt gctctcgcgc
    13801 cgatgtggca ggcgtaagtc accgtgctat gcgaaatcac tagctctcca tcatctacga
    13861 attgcccggc ctaggggtcc gcctcgctgt accagccaag caataggtgc tgccggtgca
    13921 tgagccacgg aaaacttgcg cctccaggtg atacctcaat ccacggactg gctgacgaat
    13981 accgccacta tttggacgcc attttgacct tgtgcggtaa ccgacgccca gggctcagtg
    14041 agagttaagc cgataaagat cgccagggag ccggcaagag cggatgactc gcagtgtgaa
    14101 agcccgcgtt gaatccggca ttagcggcgc tgtcctcgtc gtttaagcag ccaactcacg
    14161 cgggccacgc tgttggcgat atcatcactt agccgtggcg gatggtcgaa accgtcctta
    14221 ccggtacggc cagggcgccg ctccagccgg cgaagctctt caatcagctt actatggtcc
    14281 agcaattcga tttgtttgcc gttaaccgtg agaatgccga gattgagccg agtaagctct
    14341 tgtgacggat ggcctcgatg atgtcgggtg ttttcttgga tgcttaggaa gcatttggaa
    14401 ctggtaacct caattcctcc tgaatttgtc gtccttgcaa agaaagatag ttgtgatatt
    14461 aacccacggc attttttgcg tggacatcgg cccccagctg gatcggagaa gcaatgaagc
    14521 cattcctcgc gcatatctcg gatatcttaa atagatgtca aacgtgtctc agcgtaacag
    14581 cgcagcgatc cgcggaagcg cggaggtatc ggcaagacat cctaatcatc tccgcagcag
    14641 tgcttgtggt tctagttctt ttctttattg gtgcatacgt gatcaccagg ttttaagcag
    14701 gctgccttcg ctgcatttag tcgtccggag gaaaaccgca atactcaaaa tcatcaaggc
    14761 ccaaaagcac tgctcaaacg acgtcaggag actctggtta tctcagtgtt ggtgatcttg
    14821 atcttgggct ttatttcctc gctgcatact tttttatttt gacccttccg ttgtagcgtg
    14881 tatggatctt ttctttgagt tgatcattgc ggctgtcgcg ttcattcttt tcgcaaaggt
    14941 gatcttgctc ctctggggtt caccatgatc tacttcagca atttcacggc cgccgctttc
    15001 gctcgatgat tgcgccgctg aaaaggtcat caaagatgcc catagtgatt tctcgtttag
    15061 gcggtcggat tctttaagcg tgggtgcttc ttaaacctct tggaagacgc agcgcttgtc
    15121 actcttatag tgcttattgt tgtcacgtta tcttaactta ccctgttcgt actcaacccg
    15181 aaccgataac ctacttcagt aggccagcaa cgccgtcacc ttcgccatcc tgtcatcggt
    15241 ctatacgcga ttgtgctaag atctagtttg ttccctgctc tagccccgca gtctttgaac
    15301 tcataacgat accccttgcg accacacctc aacccgacag tcaccgctaa ttcaccacct
    15361 ggacaccgct tcgtcaccga tctggctacc gcttagatga tttgaattac ttaacggtca
    15421 ccacagtcac gactgaaaaa agttagagag agtctctatg tgtttgaatc cctaatggag
    15481 tgcgcccttg caactggaga catttcactg agatttgagg gagaggcgac cggcagtttt
    15541 tgcggttgtt tgaattgcca
//
LOCUS       DASUVS010000010        65419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_221493, whole genome shotgun sequence.
ACCESSION   DASUVS010000010 DASUVS010000000
VERSION     DASUVS010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 65419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65419
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221493"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(661..1385)
                     /locus_tag="VF182_00420"
                     /pseudo
     CDS             complement(661..1385)
                     /locus_tag="VF182_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1695..5393)
                     /gene="metH"
                     /locus_tag="VF182_00425"
     CDS             complement(1695..5393)
                     /gene="metH"
                     /locus_tag="VF182_00425"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX6435561.1"
                     /translation="MTETEKTLRELLAHRILVLDGAMGTMIQAHRLDEEAFRGQRFAN
                     HSRDLRGCNDVLCLTQSELITEIHLQYLQAGADIVETNTFNSTSVSMADYKLEADVYD
                     INVAGSRAAKKAVEAMIGKDPSRPRFVAGAIGPTNRTCSISTDVHSAATRGVTYEELV
                     QAYYDQARGLIDGGADILLVETIFDTLNAKAAFFAIATLFEERKLELPLMASVTFIQP
                     GSNRGVTGQTVEAFWNSISHVPLLSVGMNCALGPKEMRPLIEELSRIAPVYISSHPNA
                     GLPDPLLPTGFPETPQSLAPQLREWAENGWLNVVGGCCGTTPAHIASIAEAVEGLPPH
                     VPPAPEPYLRLSGLESVTIRPESNFVNIGERTNVTGSPVFAKLILSGDYEKAVSVAHQ
                     QVEGGAQIIDINMDEGMLDSKAAMEKFLRLIAGESEIARVPVMVDSSRWEVIEAGLKC
                     LQGKGIVNSLSMKEGEEKFIAQAKRVRRYGAALIVMAFDERGQADTYERKIEVCARSY
                     KILTEQVGFPPQDIIFDPNILTVGTGIEEHNSYAVNFIEATRWIKKNLPLAKVSGGVS
                     NISFSFRGNNPVREAMHSAFLYHAIKAGMDMGIVNPGMLAVYEEIPKDLLELVEDVLL
                     NRRPDATERLVTFAETYKQAGKTAVKDDGWRGGTVEERLSHALVKGLTDFIDQDVEEA
                     RRKYDKPLSVIEGPLMGGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAYLTPFLEEEK
                     KSAKEVDKRIKLVFATVKGDVHDIGKNIVGVVLNCNNYDVIDLGVMVPADKIIDTAIE
                     EKADIIGLSGLITPSLDEMVHVAAEMDRRGLVIPLLIGGATTSKRHTAVKIAPSYKHE
                     TIHVIDASRAVPVVGALISPEGRQALSEKNRREQQEIRTQFENRVGTQLLTYEEAARR
                     RFITQWDKVPIPVPEFIGARTLRDFPLSELVPFIDWSPFFHTWEMRGRYPDLLNDPVR
                     GPAAQELFDNARELLSEIVEQKLFSAHAVYGFYPANSEGDDIVLYTDATRKQELKRLH
                     TLRQQKESQRGKPQLALADFIAPRDSGVLDCIGAFAVTAGHGTQELVERFEKDHDQYN
                     SIMAKALADRLAEAFAEYLHKRARCEWGFGKDEQLNTQDLVAERYRGIRPAPGYPAQP
                     DHTEKPIIFDLLKVSDATGIALTESFAMFPAASVCGLYFAHPESRYFAVSSIGKDQVE
                     SYAARKGMKVPEVERWLASILGYDPGKR"
     gene            complement(5393..6373)
                     /locus_tag="VF182_00430"
     CDS             complement(5393..6373)
                     /locus_tag="VF182_00430"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6435562.1"
                     /translation="MDIIRTIKALGDPLRLRVLAAVAQEELTVGEVQEVVASVQSSVS
                     RNLAILREAGFIQDRKEGTNVYFSARRDMPAPAQELFKSLRERIGDLPQAKGDRARLE
                     NCLRRRLSRSRDYFETVAGDWERIRKSYFDDRVASLAIEKLLPRNMVLADVGCGTGSL
                     TFELARFAGKVIGVDLSQEMLRRARELANQKKVTNVDLRLGDALKLPIPTHSVDAAFC
                     VMVLHFLADPEKAIAELCRIVRPCGSIILVDLVQHKQEWMREQMKHRWLGFDRDAIED
                     AFRANGVKGVEYELTGSYAGEKMVRNGNQPVEIFVARAGLAKLPISRKAK"
     gene            complement(6613..7212)
                     /locus_tag="VF182_00435"
     CDS             complement(6613..7212)
                     /locus_tag="VF182_00435"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6435563.1"
                     /translation="MASDDQTRWDRQYSSSAGADQPSDYLRQILESDAWHISRGLALD
                     VAAGKGRNAILLAEMGFSVVGIDISTVALEEARRRADEKSLEITWLQADLEHIELLTG
                     AYDLIVNFNYLQRSLFPQFRRALRPQGHVIFETYLIDQQAIGHPKNPAYLLDHNELLD
                     HNELLDHFRSLRVLCYREGQYSDRGEPAFRAGIVAQKIR"
     gene            complement(7362..8129)
                     /locus_tag="VF182_00440"
     CDS             complement(7362..8129)
                     /locus_tag="VF182_00440"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX6435564.1"
                     /translation="MKPQCHPSFAERVDSLVDWLYPPRCRACSGSIQGRDREYFCSIC
                     WPQIQLVAHPFCTICGRPFPDSSGDDHACGVCLSRNPQFARAHSWACYPREETTEHPL
                     RQVIQKFKYGRKVALGKPLGRLMAHGCEEFLLTCKAETIIPVPLHPRRLRWRGFNQSV
                     LLARQIGRAYNLPLDCFALYRKMDTPPQTQLPEEDRRKNMRGAFALSGKTAVKGKCLL
                     LVDDVYTSGATVNECSRVLKQGGARAVFVLTLARAVY"
     gene            complement(8200..9759)
                     /gene="gpmI"
                     /locus_tag="VF182_00445"
     CDS             complement(8200..9759)
                     /gene="gpmI"
                     /locus_tag="VF182_00445"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX6435565.1"
                     /translation="MKGNIVATNQPVILVVLDGFGINPKKEGNAIANASMPNMDRLLR
                     NYPSSSLSMSGLDVGLPEGQMGNSEVGHMILGAGRIVYQDLTLIHKDIDEGNFGNNPI
                     ILDALKNSKAGAGRLHLMGLLGDGGVHSHQRHMEALIDMARCEKLGRVYLHLFLDGRD
                     TPPNSAEQFILDLNEKLKAWPDVKIATLIGRYYAMDRDKRWDRVEKAYLCLTEGAGKL
                     ADSPLEAIRNSYKEGVTDEFVLPTVIRSAVPEGLIRDGDGVIFFNFRADRARELTRAL
                     IDADFKEFPRTRCVKLATYTTMTQYDETFRVPVAYPPRELRKILGEVASQHGLKQLRI
                     AETEKYAHVTYFFNGGEEKEFPGEQRILIPSPKDVPTYEFKPEMSARQVTEALVKKFT
                     EEHINLVIANFANADMVGHTGNFEASVKACEVIDECLGKVVDAALSQKGRVVITADHG
                     NIEQLIDYDTGMPHTAHTINRVPVILVDEERKRSRIKEGTAIDVAPTVLQLLELPQPS
                     EMTGHSLIIDT"
     gene            complement(9818..10231)
                     /gene="rsfS"
                     /locus_tag="VF182_00450"
     CDS             complement(9818..10231)
                     /gene="rsfS"
                     /locus_tag="VF182_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX6435566.1"
                     /translation="MNKPAEKTSKERALTLVGAALDKKASQLVLLEVREFTSIADYFV
                     ICSGRSDRQVQSIAQGIHEAAREAEIAVVSVEGTSRGHWVLMDFADVIVHVFYQPVRE
                     FYDLDGLWADAPKVQLPEPYSTLVHQFQIAAEQHP"
     gene            complement(10228..10881)
                     /gene="nadD"
                     /locus_tag="VF182_00455"
     CDS             complement(10228..10881)
                     /gene="nadD"
                     /locus_tag="VF182_00455"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX6435567.1"
                     /translation="MRIGLFGGTFDPIHWGHLRSAEEVREAFDLDRVFFIPASIPPHK
                     RGQTRTPARHRLAMVRLAVAKNSRFTVSTVEIARPGVSYSIDTIRYFAEDQRKGDSLY
                     FIIGLDAFAEIGTWKDFEKIFPFCNFIVTSRPGNGYASPLRGTSIAVRRLFCYDFKRK
                     IYTHRCGTSLHFLKLTDFAVSASDIRERVRYGKSIRYLVPSSVEAYIENHGLYKRKK"
     gene            complement(11030..12310)
                     /locus_tag="VF182_00460"
     CDS             complement(11030..12310)
                     /locus_tag="VF182_00460"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX6435568.1"
                     /translation="MKKSTLANASFAEQAIQLGRQAKAAARRLAVLSSEDKNQALQLM
                     ADGLEAQSAFLIDENQHDLNAAKKAKITGAVLDRIALTSSRVQSMAKGLREIAALPDP
                     VREIVKMSRRPNGLQVGKMRIPLGVIGIIYEARPNVTADAAALCLKSGNAVILRGGSE
                     AHNSNQAIGKVLQTACGVTAVPKHAIQVVESTDRALVHELLQLESYIDLIIPRGGEEL
                     IRAVVANSKVPVIKHYKGVCHIYVDVDAELDMAERICLNAKVQRPSVCNSMETLLVHQ
                     AVASKFLPALIAKYQAAGVEIRGCDKTSALVPDIKKANEEDWTTEYLDLILAVRIVKD
                     MDQAIAHIEQYGSEHTESIITTNYRRSREFIDRVNSSAVMVNASTRFNDGGELGLGAE
                     IGISTSKIHAFGPMGLEELTTTKFIVFGDGQIRE"
     gene            complement(12307..13443)
                     /gene="proB"
                     /locus_tag="VF182_00465"
     CDS             complement(12307..13443)
                     /gene="proB"
                     /locus_tag="VF182_00465"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX6435569.1"
                     /translation="MTQRDHKKSIVRRARRVVVKIGSQILSSSTGIEEARLEALVSEL
                     AALHDQGKEIVVVSSGAVAAGMTRLRRKERPQSIPEKQALAAVGQIRLMALYERAFGK
                     FSKNVAQVLLTHEDLANRQRYLNAKHTFQMLLASSIISIVNENDTVAVEEMKFGDNDH
                     LSALVATLLEADLLLILSDVAGVYDRDPRLDSNAVIIPLIEDIKDIKSKVIGSSRSVF
                     GTGGMATKIAAAEEAAAAGIPTVITSGLIAGSIAGVFDPNEEVGTVILPDSNRLTNRK
                     HWIAFNLKPAGEVVVDSGAHEALVRKKTSLLPSGVREVRGSFGVGECVRCVDLDGREF
                     ARGLINYSALELNQIKGLHTSRIEQALGYKPFDEIIHRDDLVLL"
     gene            complement(13424..14467)
                     /gene="obgE"
                     /locus_tag="VF182_00470"
     CDS             complement(13424..14467)
                     /gene="obgE"
                     /locus_tag="VF182_00470"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX6435570.1"
                     /translation="MKFIDETKIRVIGGDGGRGCVSFHREKFISRGGPDGGNGGTGGD
                     VIAVADPQLTTLLDLRYHKECKAGRGQHGMGSDCHGRRGDDCIVKVPVGTIVRDTRTG
                     ETIGDLDKAGDRIVLAAGGRGGKGNAHFVSSTNRSPRFAQPGEPGEDRELEIELRLLA
                     DVGIIGVPNAGKSTLISVISAVRPKIADYPFTTLVPNLGVVSYNEGKSFVVADIPGLI
                     EGAHHGHGLGDKFLRHVTRTSVLIHLLDGSRIDPKRPLADWSTINRELELFDEDLARK
                     PQVVVANKIDIPEARENAKLIEEKLPDPYRPLYLISAVTGEGVRTLMQKVGMHVERAK
                     RVKEEEAGDAARS"
     gene            complement(14637..14894)
                     /gene="rpmA"
                     /locus_tag="VF182_00475"
     CDS             complement(14637..14894)
                     /gene="rpmA"
                     /locus_tag="VF182_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006919250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX6435571.1"
                     /translation="MAHKKAGGSSRNGRDSKGQRRGVKVFGGETVRAGNIIIRQLGTR
                     IHPGRNVGMGRDFTLFALIDGVVNFDRLDRSRKRVNVLPVQ"
     gene            complement(14919..15233)
                     /gene="rplU"
                     /locus_tag="VF182_00480"
     CDS             complement(14919..15233)
                     /gene="rplU"
                     /locus_tag="VF182_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX6435572.1"
                     /translation="MKYAVIKTGGKQYRVSEGDLVKIEKLTGEVGEKITLGDVLFIGG
                     NGDVRIGSPLLENASVTGEIVSQAKAKKVLVFKKKRRKSYSRQRGHRQHQTTLKITGI
                     DG"
     gene            15430..15909
                     /locus_tag="VF182_00485"
     CDS             15430..15909
                     /locus_tag="VF182_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX6435573.1"
                     /translation="MPDFKAMAELRYQIRKFLRFSENAARQAGIEPQQHQLLLAIRGL
                     PDAASPTIGVLAERMQLQHHSTVELVDRLVDRNFLCRLRSTNDRRQVLVKLTHDGENF
                     LEKLSLHHLEELQSAGPTFVKVLNSLLADSLIDNPNSASEVAKETVTDKPRKDRNHG"
     gene            15902..16750
                     /locus_tag="VF182_00490"
     CDS             15902..16750
                     /locus_tag="VF182_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6435574.1"
                     /translation="MADQSAEELLLDVRVAEASRLYKFYLNNEQKILGTASVVTFLVA
                     WELVGNVLQLINPMFMSAPSLIFKAAIDMFSSGEIYNDLYVSGIELFWGYVLSAVVAV
                     PFGIMVGWYKRASYIFDPFINAMNATPRVALLPLVIIWLGIGILSKVGIIFLGAVFPI
                     LINTRDGVKTTPYNLLTAARSFGASEWMLFKTVVLPSTIPFILTGMRLGLGRAIVGVM
                     VGELYAATAGIGFMITVAGATFQTDKVFVGVLIFALTGMIGMEVLTRIERRFDKWRPK
                     VGAQEI"
     gene            16908..17504
                     /locus_tag="VF182_00495"
     CDS             16908..17504
                     /locus_tag="VF182_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX6435575.1"
                     /translation="MRNLTSGFYLLRSSMILLVLTATGCGYNTLQSLDEEVKGAWGEV
                     QNQYQRRADLVPNLVATVKGAAKFEQDTLTQVIEARSNATATKIDPETLNNPEAFKKF
                     EQAQNQLSSALSRLLVVVERYPELKANQNFQNLQAQLEGTENRIAVARKRYVERVAQY
                     NTAVRSFPTNLTAKFLGLNVRPSFSADEAAQKPPQVKF"
     gene            17568..18344
                     /locus_tag="VF182_00500"
     CDS             17568..18344
                     /locus_tag="VF182_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX6435576.1"
                     /translation="MSTPHKFAFFLFLLCLLSTRAYPLDVPPLRGRVNDYAKVITADQ
                     TRKLEDQLAQFERDSGHQVAVLTIPSLEGDSLEDFSIRVAENWKIGKKGFDNGVILIV
                     VPKDRRLRLEVGYGLEGVLPDAIANQIISQYIIPRFRAGDFSSGIVAGTDAVLRVIKN
                     EPLPEADRQKPARPSGSGFGSLTGLLLLLGFIAVMSMASNTNRRRQGLWTTRGRRPPP
                     IFWGGGGFGGGGFGGGGGFGGGGFSGGGGGFGGGGASGSW"
     gene            18344..18973
                     /locus_tag="VF182_00505"
     CDS             18344..18973
                     /locus_tag="VF182_00505"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX6435577.1"
                     /translation="MMRPDKFLTEAERERIHQAVIAAESKTSGEIVPMVVTSSARYNE
                     IELYGIVSGMFLGMLLGWLWHDPWGSYRLNVWPAVGATLGFLIARIPAVKRGLTSSSR
                     VEEAVNNRCMAAFTEHGLHYTRDHTGILILVSLLEHRVEVLADRGINEKVPPGTWNEI
                     VEILTAGLKSRHAGDAFCKAVERCGEILASHFPRQADDRDELPNRLVSD"
     gene            complement(18989..21046)
                     /locus_tag="VF182_00510"
     CDS             complement(18989..21046)
                     /locus_tag="VF182_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX6435578.1"
                     /translation="MTSFQRRPVENVLSAKSVAIVGASPKGRWPMGIFRNLKKAYTGK
                     IFLINPNYQEIENYPCYPNLAALPEVPEELLILIPTRAVLGTLEEATKLGTKSATIYT
                     AGFGEGDDPKGKERAQAMKDLCERTGLVICGPNCMGSYSLSEGLWTFPTSTPLLKKGP
                     VGLIFQSGGSLGNWIKGATERGIGFTYAVSSGNEISLDLVDYLSFMVDDPGTKVILLM
                     AEGIRRPNEFMALAHEALNKNKPILVVKLGRSEMGKRQAISHTGALAGADEVFDAVCN
                     RLGLVRCPTLEDLTEMTLAFMAGRYPRGSRAAIVVNSGGMKGLICDHAEELNTNLAQL
                     SDKTKEAVRPLIPPELAVENPLECGVAGFGDEQGFINIVKLHAEDDAVDLLAIHGELP
                     RFPEKREATLFKNLAAATDKPILAFARSTYSCTDESRTFQEQAGIPFLQAIKPTLRAL
                     AGLGLYGERKRLGVPTLPEANGNRDELEGDKLNALLQSCGIGLPKQFLVGTAAEAGLK
                     SKEIGFPVALKLVAAEIVHKTESGAVVLGLKSPEEVVAEGQKLLSKTLGKGHLLVQEM
                     VQGTEVLLGARMDPQYGPFLMVGLGGILVEVLKDVAIRLLPVDEAEARRMLQELRGSK
                     VLQGVRGQGPRDIDALVKAMIGLSDIFAAHRNHLADLEINPLMVRAQGRGAAAVDVRL
                     VRK"
     gene            complement(21171..21725)
                     /locus_tag="VF182_00515"
     CDS             complement(21171..21725)
                     /locus_tag="VF182_00515"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX6435579.1"
                     /translation="MEISKIFLNGRIAVKIGDITKEQVDAIVNAANGTLMGGGGVDGA
                     IHRAGGPEILKQCKEIRAKEFPQGLPTGEVVMTTAGRMPAKHVIHTVGPVYGRGGGEK
                     AALLASCYRNCLRLAVEHGLKTIAFPAVSTGIYGYPLSEAAKVSSETIEEFLGSNASL
                     EQVRLLFFSVDDADAFLKNQAFTY"
     gene            complement(21853..23541)
                     /locus_tag="VF182_00520"
     CDS             complement(21853..23541)
                     /locus_tag="VF182_00520"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6435580.1"
                     /translation="MFDLLIKGGRIYDGSGMPSFSGDVAVAGGKIAAIGRLDGPARRT
                     LNVEGLAVTPGFIDPHTHLDAQLLWDPLGTSSCFHGVTSAVVGNCGLSLAPCKPDGRE
                     AVIKSFVRVEAISRKVLEQGIKWKWVSTGEYLDALGSHLGINVAALIGHIAVRHYVMG
                     EDAVEREATPEEIARMQQLVREGIAAGAVGFSTNQNPRHMREDGKPVASRVASHQELL
                     ALLEVLGDMNTGVVQLSGGGVDARGRIAYASELARATGRPVLWQSISHSWTRPDHWKG
                     MLESTARVFREEGLPIYAMTQAKPFENRYTLLDAQCFDEFPTWRAAMFMPVEQRQTIF
                     ADAEARQKMRAEVNSPEPSVFPKRWDVIYVDEAKLPKHKSLQGRTLEEIARSRGKDPL
                     DVFLDIALEENLETRFSHVTTQGDPNAVCEILKNPFVIIGQSDAGAHMGYDARFGYCT
                     TFLGRWVRDNGIMSLEEAISKLTFQVASIFGLRDRGLLRSGLAADITVFDPHTVDTMA
                     PEYVQDLPGNERRMIQRATGVHHTLVNGEVVIENGAVTGTYPGQVLRNTPAPRD"
     gene            complement(23677..24417)
                     /locus_tag="VF182_00525"
     CDS             complement(23677..24417)
                     /locus_tag="VF182_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX6435581.1"
                     /translation="MGTETFLVRTMSDTVETDIQRVLTITTEILPKYGLHPLPFERLR
                     KIADGPFDLFLIPLVFADEYKEDTNLIFNEKKRLEIRTQALEIARKHSFDTKPPNFVP
                     GIEESLKSTKSAGLRNVLLTTGGRRYQHHAMETFGIGKYFAEIVDRDETYYAKEQGLY
                     YLFREHKLPSLRLILLSGTASYIRAGNNVDGCKVAGNHVEVVTIALSNGYSYNDEETL
                     MAAKPNMLIHSQDQLLPLLKENGLVPGA"
     gene            complement(24563..24844)
                     /locus_tag="VF182_00530"
     CDS             complement(24563..24844)
                     /locus_tag="VF182_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX6435582.1"
                     /translation="MEPTSFLVSVSEEEIRRERRKARELRASQWWKRKRALGLCHYCG
                     GKFPPAILTMDHLVPIIRGGKSTKGNLVPSCKKCNSERRYRLPFEESPG"
     gene            25162..25542
                     /locus_tag="VF182_00535"
     CDS             25162..25542
                     /locus_tag="VF182_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6435583.1"
                     /translation="MSREIVQTNEAPKAIGPYAQAIKANGFVYTAGQIAIRPDSGELV
                     DGGIAAQTRQVFANLAAVLKAAGTSMDQVVKATVFLRDMTDFAAMNEVYAEHLGKATP
                     ARSTVAVTELPRGALIEIDLIAEV"
     gene            complement(25558..26142)
                     /locus_tag="VF182_00540"
     CDS             complement(25558..26142)
                     /locus_tag="VF182_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX6435584.1"
                     /translation="MRKERAEVVRCPQCEVPMKPVSVRANPGSLIELDQCGQCGGIWC
                     DKWELFPIQADEAARLEPADEKLLRLPLVLQKEIFCPRCTARMSAINEPSLGADVQFR
                     RCFKCDGIWLKRGQFTRYKNHQEKVRSKNIGAKTTGKKLPETDGDEKSWVVTGTKGMF
                     AYPPSEETIEAGSDSLTNIVETVLQKLLQMVSRT"
     gene            complement(26123..27058)
                     /locus_tag="VF182_00545"
     CDS             complement(26123..27058)
                     /locus_tag="VF182_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX6435585.1"
                     /translation="MFCFLFFFSLIGLSIDYLYLDAFGARGASFPIATLIALCISVTV
                     AYTGYRQGSKLILSSVGAEPLDIQIPEHRELHNIVKEMALASGCRMPKVYVTYDPAPN
                     AFATGRNEESASICVTTGLLTLLGREETQGVVAHEMAHIKNNDTSVMLLITVLLGGVV
                     LLSDWAQRSICGARPVRRPNAQALFAIPAVLLIILSPLISRLLAMAVSRQREYLADAT
                     AVEFTRNPQGLANALEKIRDAGMPFQKATRGTAHLFFINPLRRRTDGSDGKLADLLSS
                     HPPINRRIMLLYQMGALPRPAAQFGVETIDAQGTR"
     gene            complement(27137..27700)
                     /locus_tag="VF182_00550"
     CDS             complement(27137..27700)
                     /locus_tag="VF182_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX6435586.1"
                     /translation="MTWLFWAVIFLIAVWLIWTYNGLVALRHQIENAWKQIDVQLKRR
                     YDLIPNLVETVKGYMQHEQDTLQKVIDARSRAMTSTGIKENAEAQTALTQSLGRMFAL
                     MENYPDLKANQNVLRLQEELTTTENQIAFARQYYNDLVMRYNMKQEVFPSNMIASFFR
                     FKPSEFFSAPETERAAPRVSLKLSLLF"
     gene            complement(27697..28725)
                     /locus_tag="VF182_00555"
     CDS             complement(27697..28725)
                     /locus_tag="VF182_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEX6435587.1"
                     /translation="MIEKPLEQMGLRDLREIARRTMPAEAWHHFNGAAETKATFHRNP
                     RAFRQYMFRQRIFHDITDPDIAIELFGHKLPIPAITAPVGSFSLIGKDAEREVAEGTD
                     RVGAMMFVSQAAKFNPRDWRNAAKSPFVFMAYMNRGREEVTEYARLAQDLGFAAVGIT
                     MDTVRPVKIGDEVPLSTKDGKPRKGHKSSPKDIEWLKQQVSLPVIVKGIMSAEDAHDA
                     VNAGADALVVSNHGGRILDFNRAALEVLPEVVAAVGAKVPVLLDSGIRSGGDIVKAIA
                     LGARAVLVGRPIAWGVGAFGARGVARVFAILAEEMQRVLTMTGVASLGDLTQSILVRD
                     ELAGRWGR"
     gene            complement(28730..29581)
                     /locus_tag="VF182_00560"
     CDS             complement(28730..29581)
                     /locus_tag="VF182_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6435588.1"
                     /translation="MIKPIALSATHMECRNLRESMAVLIDLLAFEKIAEKAGEAILKH
                     PNTHWLLFLHEAGPDAPTKQMHNHWGVRVASIDEVDRAYEYLNAHKAQYKLGAIGKPL
                     WNHGSYSCYFVEPGTNGWEIESYEVVNRKESAKAKAGGVRGPHWTSPFPEERFPGRGY
                     VPQGFTHGTLVARDMNVNRRFYTEVLSLEVHQANDHVIYVKHPDTKTYIVCAERKEFK
                     VFSPNFRNTLSVKSNEAVKKAHRLFSEQGDSLGVTELFPIDENSSSNSFFFRDPGTNC
                     WEIASQN"
     gene            29889..30917
                     /locus_tag="VF182_00565"
     CDS             29889..30917
                     /locus_tag="VF182_00565"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6435589.1"
                     /translation="MRLLFRGAVLIMICVLPNAPAATFAQESFYQGKTIRIIVGTSAG
                     GGFDAYTRTLARHFGKHIPGQPAVVVENMPGAGHLIAANHMYNVAKPDGLTIGHFHGG
                     WFLYQLFKRSGLEFDATKYEFLGSPIRETRACALTKASGITSVERWLAAKTPVKIGGI
                     GGGAPDDIARMLAATTALPVQLVAGYKGTSEIRLAAESGELAGGCWTWDSIRSTWSKG
                     IQSGEAVVVLQLVSKPHRDLPKVPLAQNLAISEEARQLLKAGIQDTAEFYRPYVAPPR
                     TPKQRIETLRQAFDATMKDGEFLADAQKANLEIEPITGQEMERLVSGIFKLDPALAAK
                     LKSILNPS"
     gene            30964..31989
                     /locus_tag="VF182_00570"
     CDS             30964..31989
                     /locus_tag="VF182_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6435590.1"
                     /translation="MQKLLLALSMVLLGGFAAESDHALAQANFFEGKAIRLVVGFTAG
                     GGYDAYTRTLARHMGKHIPGNPSMIVENMPGAGSMISANYTFKAARPDGLTIGHFIGG
                     LFLQQLLGKPGIEFDAAKFQFVGVPAQDNFVIGLAKSTGITDVESWMASKQVVKFGGV
                     ASGSGSDDFPNILKATLGLPIQLVSGYKGTADVRLAFNSGEVAGLSNSWESTKSTWRK
                     ELDSGELKLILQATLKPHPEHAKLPMALNYAKTEEAKRLISTVARVHGPTVRPYVLPP
                     NTPADRVQTLRQAFMATMKDPEFLAEAKKANLDINPEDGATLEQNVKEILQLEPALVA
                     KLKEFLK"
     gene            32119..32337
                     /locus_tag="VF182_00575"
     CDS             32119..32337
                     /locus_tag="VF182_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX6435591.1"
                     /translation="MKVSVDRTKCEGYARCVQAAPKVFQLDNKMIAEVVDLQGDSDDK
                     ILLAARLCPTKAIAVEEEGTGKKLFPVE"
     gene            complement(32389..33111)
                     /locus_tag="VF182_00580"
     CDS             complement(32389..33111)
                     /locus_tag="VF182_00580"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435592.1"
                     /translation="MPYDCWRGMVGMIKPTKGSGSLVELIKMLPDGIGVIPLFNNVRH
                     GKIEEFQSAIPAYEEKIAELAQDQVDLIHPAGTPPFMLLGYKGEAEIIQRWERRFGIP
                     IFTSGTNQVAAMKALKIKKFVGIGYDFDDTSIVAKYFTDAGFEVLELGKLPGPWEEVG
                     RLSSKDIYFQAKRLFLRHPDADGIYFQGGKLRILDIVEELEQDLGVPVIHPGVTQCWE
                     IQRRLHVRVPRSGYGRLLSELP"
     gene            complement(33729..35117)
                     /locus_tag="VF182_00585"
     CDS             complement(33729..35117)
                     /locus_tag="VF182_00585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX6435593.1"
                     /translation="MSFRKSPLTDIGTHSAKLKANDLKSREKFNAYRFADVQLPKAVV
                     VFFAITITVLAFLSINNYFRGSTKDYAKWFLIGETVLKGGDIYAPRGDGTFQFMYPPT
                     AAVLFAIPSYFGELPLIIVLVLLYSVAWVASSLLSVSLVTGNAARAQSILYLLPGICS
                     LPYVTENYILGQPNLLLLAFMLGAFACLRSGREWSAGALVALATAIKAFPVLAIGYLI
                     YRRHWKATLSASVFLFLLVVALPSPFRGFERNLEDLNTWTSGMFRYDNQSISQREQRG
                     FSWANHSLVAVAHRLLRPVNAHRKQDQQLFVNIADLDFKYVTIVVVLVGTALCVFYLF
                     CMPSYPERTKESDALEYAMLLLLILIFTPLAFTYFFVWLLYPLVVALCIVLRAPQGSL
                     QRIAGWSWFFVSVLFLCFTFPLAAFRAVQAAGSTLLACLLLLGGIGWKLRAETRNTLK
                     PKLTHEAKTVCI"
     gene            complement(35120..35866)
                     /locus_tag="VF182_00590"
     CDS             complement(35120..35866)
                     /locus_tag="VF182_00590"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6435594.1"
                     /translation="MRLENKVAVITGGAQGIGRAIAMGMAREGARVVVADLQSEKACS
                     VADEVKVLGGDASGFEVNVADELSVKKLAEATFANFGRIDILVNDAGVYLKSSVVDMS
                     EADWDRTLDINLGGNFLCCRAFVPAMREQKSGRIISMASGIGHYGMKQFSHYAASKAA
                     IIGFVKSLARELGPDGITVNAICPGSANTAMPRGHRSEEEVMQRLHSTPLPHVLEPED
                     IAGPVVFLATDAAAYITGQCYNINCGTYMF"
     gene            complement(35863..36606)
                     /locus_tag="VF182_00595"
     CDS             complement(35863..36606)
                     /locus_tag="VF182_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6435595.1"
                     /translation="MDQIALIVGGGGGIGGAVAHRMAAAGITVAVADYDRAAAEQVRH
                     EILAARGKADAFSVDVTQAGEVKAIVAEVLSRHRRIDVLANIAGGSIYTKRIEEFTWN
                     EWKQVIDSNLKGTFLMCREVAPVMQKQKSGRILNTASNYGVTGSALRAPYAAAKAGII
                     TFSKSLALELAPDGILVNTVAPGPTDTPRVMEKESPAARRERWYAAIPLGRTGQPGDL
                     AEMYYFLTTPESAAITGQTLHVNGGVVMP"
     gene            complement(36784..37224)
                     /gene="mscL"
                     /locus_tag="VF182_00600"
     CDS             complement(36784..37224)
                     /gene="mscL"
                     /locus_tag="VF182_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018977839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX6435596.1"
                     /translation="MWKEFKEFIARGNVIDLAVAVIIGAAFGKIVTSLVDGILMPPLG
                     LLLGKVDFASLFYVLDSSKGIPVSLQDAKAKGVPVIAYGNFINDVIYFLIIAFVIFLL
                     IRMINSMKRKEAEAPTAKDCPYCLSTIPVKATRCAHCTVDLKAA"
     gene            complement(37363..39171)
                     /locus_tag="VF182_00605"
     CDS             complement(37363..39171)
                     /locus_tag="VF182_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6435597.1"
                     /translation="MSLFSASAVAQNSWSSLGQLFFRRVGEVGCRTFLKIQRCGSFED
                     KSWRDCGAMVQSALLGLHELRVAKGDNVAIMGDNSLPWLCADLATLAGGFPNVVISPN
                     VSDLTLLKILAHSRCRAIFVDAVSAPRLLAIKAQLPNLSLVVVLDGSAEELRGCISFG
                     ELLERGRRSHISELDRLLQAVQANDLATIMYTSGSTGEPKGVMRTHNNLLANFASRGE
                     IPLSKAEELTIIVLSLNHLFGRFGFLKSAVTGRTTAIMEAPEQNLDLAVIQGLRPTAM
                     SVVPRVMERLWNAILDRGVIRELWERIEKLDRDDRLMGSDDHAVGEIAATRTNLRQAA
                     QQALGGRVKYITYGGAAMPPRILRFFDLIGVPLIGSYGSTECGGVTLCGVGNVKPGSL
                     GTPFPNVELRLADDGELLVRGPTVMPGYFDDPQATCEAFDADGWFRTGDLGKIDNDGS
                     LFIIGRKKDVFNCADGSNINPGTIEALLEDDSFVRQAILVGDHRPFIAALIVPERPNI
                     AAALNRMETTLSDSDLEGALRQRVDLINTKLDRYEQIRKIAVIPNDFPKQLRSITALQ
                     KIKIDRQAVGERYRAEIEAIYSCGAEELQVPASESS"
     gene            complement(39168..40541)
                     /locus_tag="VF182_00610"
     CDS             complement(39168..40541)
                     /locus_tag="VF182_00610"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HEX6435598.1"
                     /translation="MSERAVGVAFSVPYEIASVRHLRQTSLQERERALKACGSNCELL
                     PEDAIYVDLCTDSGVSALSTAQIAAGNGSEALEPAMGMATEASRAFASLSMQYRKFFG
                     FPYLVPTTQGRAAERIWAKLHVKPGTVVPGNLLFPSTRMHIESNGGRVIDVIGDGAHN
                     LQSEDPFKGDLDIEKLDAVFREHGPQQVSCIYIELSVNACGGHPVSLGNLKKVKAMAA
                     ANGVPLFLDACRILENSYLVRQREAGYHDRSIREIVLETCDSADGCTMSAMKDLLVPM
                     GGMIGSRDPVSFQKATLQSFLDGAQPPASAMQMIGTALEEIFAADAYVTSRVEQVNYL
                     WRRLRERIPLVHPPAGHAVYIDLAAFLPHVAPENHRAEALAAFLYYISGVRVTKGAPP
                     APAQTARGIELLRLAVPARKYVQGHIDDVAEAVLYAYAHRHEIRGLKRIEAPQRSKYQ
                     PYHFEPL"
     gene            complement(40584..41429)
                     /gene="cutA"
                     /locus_tag="VF182_00615"
     CDS             complement(40584..41429)
                     /gene="cutA"
                     /locus_tag="VF182_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HEX6435599.1"
                     /translation="MPELIVVYVTAGSPEEADRLARSLVDERLAACVNRIKSVQSVYR
                     WQGKVEDSEEELLIIKTSRDRFAVLEKRVRELHSYSVPEVIALPVIEGSSGYLKWLKE
                     ELTPGTRPRSDGIRTVREVSAGGVMYRKHSDGIQIALIHVRNRWGLPKGHVEEGERIE
                     ETALREVREETGLEGRLVKKLGDIRYAYRDKTKEGEPIRIYKRVHFFLLRYLKGDVRD
                     HDHEVDEARWFPMDQVIKNLKFATERKMVHRALNILSASDHQRIKPVLEQEPEDEPVE
                     QKLPR"
     gene            41563..42294
                     /locus_tag="VF182_00620"
     CDS             41563..42294
                     /locus_tag="VF182_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX6435600.1"
                     /translation="MMQSKEEIRQKVWSVMQREKVARFPGAQGRIPNFAGAEACAPLL
                     AEKSYWKRAKALKANPDSPQRAIRHKALTEGKIVYMAVPRLRHEKPFVELDPGKLDCS
                     PYVASSIQGAAKFGRLVGVNQMKKIDLIICGSVAVNCQGARVGKGGGYSDLEFALLTE
                     NKKVSAKTPIVTSVHALQILDEDLPMIEHDIPLSAIVTPGTIIEIDPAFPRPEGIYWR
                     MLPPEKIQAIPILKVAAATRDAATA"
     gene            42350..42736
                     /gene="hisI"
                     /locus_tag="VF182_00625"
                     /pseudo
     CDS             42350..42736
                     /gene="hisI"
                     /locus_tag="VF182_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174544.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
     gene            42774..43673
                     /gene="hisG"
                     /locus_tag="VF182_00630"
     CDS             42774..43673
                     /gene="hisG"
                     /locus_tag="VF182_00630"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX6435601.1"
                     /translation="MKAGKELKLGIPKGSLEEMTIALFRKSGWKISTTSRSYFPSIDD
                     DTLRCSLARPQEMSRYVESGTLDVAITGRDWVMENGSDVQLVCEMVYSKTSFRPTRWV
                     LAVPQDSPIQKIEELGGKRISTEIVNYTKRYFAEREIPVEVEFSWGATEAKAAEGLVD
                     AIVEVTETGSTIRAHGLKIIHELMESCPQLIANREAWQDSWKREKIEQIKMLLLGALS
                     AENKVGIKLNVPETYLEKVVELIPSITAPTVANLHPSPNLKGVKWYSVESVLEESKVR
                     DLIPKLIQNGAVGIIEYPLNKII"
     gene            complement(43734..45101)
                     /locus_tag="VF182_00635"
     CDS             complement(43734..45101)
                     /locus_tag="VF182_00635"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HEX6435602.1"
                     /translation="MAAKIELMETVLRDAHQCLLATRMRTEDMLPIAAQLDDIGYWSL
                     ETWGGATFDATLRFLKEDPWERLRRLRAAMPKTRFQMLLRGQNVVGYKNYPDDIVQSF
                     VGRAATNGIDVFRIFDAMNDVRNMKTAIEAALNTGKLVEGTVCYTISPVHSPDYFLRV
                     AEKLADMGVQIICVKDMAGMLAPYAAYEIIKKIKSRIPLPLHLHSHCTAGLSPMNYMM
                     AIEAGADILDTAIAPLSQGASQPATEQLVAALRDTPYDTGLDLTKLWKIADYFYGVRK
                     NYAEFESPVNNQIKTDVLVSQIPGGMLSNLVAQLRQQKAEDKLDAVLAEMPHVRKDLG
                     YPPLVTPTSQIVGSQAALNVMTGKRYSVIAMETRNYVMGLYGEPPGQISEEMKNQVLR
                     KKQPISCRPADLLKPGLDQARKEAGSLARSEEDVLSYALFPEIAKEYFLHRDGTGERQ
                     QATGA"
     gene            complement(45127..45678)
                     /locus_tag="VF182_00640"
     CDS             complement(45127..45678)
                     /locus_tag="VF182_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435603.1"
                     /translation="MAAEATKKRKGTALLAVMDENCSSCAGSPLCEAHCPVDECINLV
                     YEELPQGGLKPYRVMVDNDKCIGCQMCYSDDLTKVQQHKETGEIFYEYGSRFYDSNRN
                     PVPPDAVPKRFQLQLIGTESEDRLDKKICPWDAIKMYEFDDGLEVSQFFYDITKLRKI
                     DGGYVIDDAEKERLEEKQTELYE"
     gene            complement(45668..46492)
                     /locus_tag="VF182_00645"
     CDS             complement(45668..46492)
                     /locus_tag="VF182_00645"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6435604.1"
                     /translation="MLRGWLVGLAVLAWVGSSLAAETTSPLVKQKQWEEARNYLAEGK
                     AAEAKAVFEALLRRYPQEADLHLFLGITLLRLRDPQAAEASVRKAILLDPDHVEARTL
                     LAWIESEIRSDFDAAIAEYKTVVELKPDLPQAYNNLGVAFKRKGDLAAAADNFNEALE
                     RKPDFSAALNNRGWTFAEQDRWSEARRDFEQALKINPQDDGALYGLSQALREVRDYAG
                     AQEVLGQLISRSPNFVYWLEWGRIGLIRYWWVLLAIALGFFFKGRFKKARVEAHGG"
     gene            complement(46493..46678)
                     /locus_tag="VF182_00650"
     CDS             complement(46493..46678)
                     /locus_tag="VF182_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435605.1"
                     /translation="MDEASGLQNFLAIVTKPDNIPIVGMLILVIFFSWIGLREAFKND
                     KLIEQGKEDQIPNEMWK"
     gene            complement(46767..48428)
                     /locus_tag="VF182_00655"
     CDS             complement(46767..48428)
                     /locus_tag="VF182_00655"
                     /inference="COORDINATES: protein motif:HMM:NF018032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEX6435606.1"
                     /translation="MFLVLSNENLPFLRHAGQKGSLESMEDLGTDILILHEGHEDEYG
                     DDEEDIVMDEDHPMWRSERITLNSVGIDIGSSTSHLIFSRLTLRRQGMALSSRFVVVN
                     REMLHESPILLTPYVDKTTIDTNKLNDFIHDSYRKANLTPSDIDTGAIIVTGEAAKKK
                     NAEAIAALFSAEAGKFVCATAGHNLESILAAYGSGAVGMTHHEGGDFTVMNVDIGGGT
                     SKIAIAQGGKVIDTCAMEVGARLIAMDENGRINRLEDTALKVAKMAGVKLALGASLSE
                     EDKEKFAQVLCDSLFAVLERGPISSQAQDLLLTPNLEYKDPIHAVMFSGGVSEFVYGY
                     EKRNLGDLGVHLGRRVRARANQLAGGKIPLRPAEVRIRATVIGASQYTVQVSGNTIYL
                     SHPELLPLRNLQVITPEFEQSEAITSADIAAAVQKALQRFDIMDSDRAVALAIRWELG
                     PSYPLIRMLAEGLVDAMKEHVDQGQPMVLVFDADIAKLMGNIIERELIPGAGIISIDG
                     IDLKDFDFIDIGQELPDAKAVPVVIKSLIFRHNEQRRGHHHHHHH"
     gene            48514..51825
                     /locus_tag="VF182_00660"
     CDS             48514..51825
                     /locus_tag="VF182_00660"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HEX6435607.1"
                     /translation="MGQEEFQELSSFLKRFIRRFKFLKGIEGLCVTAICALLLLAVGP
                     GVDQIKNLFPYAPLVYSILTAAILLGLILWTVIQLARRFSPEHAARYVEQKQPKLRNN
                     LINSLQLYPQIVSAQAAPGFSAPMVLALLRTTRKEVSGLKLNELLDTRRVRSSLRLFV
                     LLFIPVLGMVLFNPSWVGQTFSLLTRPLDHMPPSETALEVNPQGVRVARGVPVTIEAT
                     ASGVIPKSLDLITWAGSNDRGELVGEEKMAMESLGEGKFRVKLARLERTVSYRAVSGA
                     FSSPVYSAEAIDPPEIGNVQLTLYPPAYTGLGATSSTSGNVEGLKGSNLRIAATTTKD
                     VVKAEIVLADGKQVPLKIEGRKLQGNLVLFQSQQYQILVEDRYGFRNSPISYDLKVKP
                     DGFPTIDLLRPTEDLEINGDEILTLEYSARDDFGLAEVTLSVKVGERQEKIRLQKDDA
                     RRIILRDQYRWDLGRLALREGEDALVNLEVTDNDTVTGPKLGSSRAIRLRLKNIKGEH
                     QQVAEMVRELNNRMVDLLGDHLEKPMPEENKADRGSDMNQKFEQNLAEALKQTEEVMR
                     RTEKDRLSDYATWSDLEALKRNLQFTKEELLKKQEQAQSAEENLRARDEISSELERMS
                     LLSEDISKRLKAQEMASTAQDLARSQERLMDSLEKLQSGDKNLDTILKQLSELTKSLA
                     ALQQAMSQMAQQLPDDFMNMENMRSLGFNEMFSALDEIRKKLMQGDIEGARQLARELF
                     NQMAQMVASLQNSQRSAMASSMGRMQGEMSRSTNELQQIAREQQEILLNTEDINKSAL
                     NEHDNALKDKLDRFLENARNELGKLTELFPDREGPDDPEAAAKLDDATMNQLLKNLIA
                     KLLSKDFPGYGEGDGLARKELAKKRDPSQAERARRAESTLDKLKSELNSLMGQPMQAL
                     KESDQKQLRDLANRQGVLRERTESLHEKLESLFQLFPSLDPKIVQNIGEAGKSMNNAN
                     DRLGQLDSHGAVPPERDALDRLSQSQQQMQSAMQQMAQRGQLGTMPMTRLFRQGRFMP
                     GGMLTPLPGMPQFPEFEVDSGFTGLDTEKFRLPGKEEYKAPRNFREEILESLKQGVPP
                     QLKDQIERYFKNLSE"
     gene            51870..53420
                     /locus_tag="VF182_00665"
     CDS             51870..53420
                     /locus_tag="VF182_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6435608.1"
                     /translation="MLGLTAQLLAAEVEDKFKDGQELLDAWRISQAEELAAKSVRDNP
                     KSADALGFDARVKFYQGRYQEALVSIDRALAIDSKEKQRQGLKLLTQFTVDVIKTLKR
                     YESAHFVLYLDEKRDGILATHALDALEKSYQAVGAELGYYPKDKVRVEVAPDAPSFNA
                     ISTLSLRDIEETGAVGICKFNKLMIISPRALSLGYRWVDSLSHEYLHYAIVGLTNNQA
                     PIWLHEGMARFYETRWRKPVPPKDAAEDYLTPANQTLLVQALEKNQFVGFKKMEPSLI
                     HLETPEQVQLAYAEAASAIDYINQSKGKAGVRELLATLNETSTPEAISKVLGLSFDDF
                     ESQWKNFLKQKGLKEIEGTRVRRLKVKKDQNEDQEIVELKEIQSAVARNRTHLGDQLS
                     NKGRTVAAANEYQRALEASPHSPIILNKLGRVLIQMNRFPEALNPLKKALDLDPDNGG
                     TYVQLGRIYHATKKFQESKAALEEAIQINPFNPVIFKLLTEVYASLGEKEKAQQAKAS
                     LDKLLGAS"
     gene            53432..54460
                     /locus_tag="VF182_00670"
     CDS             53432..54460
                     /locus_tag="VF182_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX6435609.1"
                     /translation="MDAAKIHQHEIAEIEEFAAKRDTMLGEIRKVIIGQDRVIEEVLI
                     ALFARGHCLLVGVPGLAKTLLISTLAEILDLDFNRIQFTPDLMPSDITGTDILQEDPS
                     TGRRQFQFLKGPIFTNILLADEINRTPPKTQAALLQSMQEYKVTAGGTTFPLDLPFFV
                     LATQNPIEQEGTYPLPEAQLDRFMLNIEIGYPEFEDEVQIVLQTTSNEKPSPRKVMQG
                     AAILRYQELVRRVPVSPFVVSYAVALSQSSRPQAGDAPQFVRDYVEWGAGPRASQYLI
                     LGAKARTILQGRYAVSIEDIQALAPSVLRHRVVPNYKAQGEGLSSVDIIQRLLSEVKP
                     PKDGKTAL"
     gene            complement(54905..55852)
                     /locus_tag="VF182_00675"
     CDS             complement(54905..55852)
                     /locus_tag="VF182_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017309096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEX6435610.1"
                     /translation="MNASLDDRSEAWPSLSLDAWRETYAALHLWTQIVGKIRLSLSPW
                     VNHSWHTTLYVTATGLTTSPIPYGTRSFQIDFNFLEHQLRLRSSDGCTAALALEPQSV
                     ASFYGHLMEEMHKLDINVRIHRRPNEIAEPIPFDQDVCWRPYDADYAGRFWRILQQAD
                     RVLKIFRSQFVGKCSPVHFFWGAADLAVTRFSGRKAPQHPGGVPNLPDWVTQEAYSHE
                     VSSCGFWPGSGPVPYAAFYSYAYPEPAGFSKAAVNPQGAFYSDDLSEFVLPYDRVRES
                     ESPDEMLLEFLQTTYEAAANLAEWDRAALERRAHPKSGK"
     gene            complement(56317..56790)
                     /gene="rplQ"
                     /locus_tag="VF182_00680"
     CDS             complement(56317..56790)
                     /gene="rplQ"
                     /locus_tag="VF182_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX6435611.1"
                     /translation="MRHLNSGRKLSRNSSHRWAMMRNMVTSLLREEKIQTTDPKAKEL
                     RRWVDRVITLGKEGTLHARRQALAIIQDKTVVRKLFETIATRFQNRPGGYTRIIKIGR
                     RHGDAAPVSLIELVASADEAAEKTSGKDKKAKKKSGKTARKASAPRRRNAQEAST"
     gene            complement(56793..57812)
                     /locus_tag="VF182_00685"
     CDS             complement(56793..57812)
                     /locus_tag="VF182_00685"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX6435612.1"
                     /translation="MYRHWTDLIKPKQLEVDEKTLTSTYGKFYAEPFERGFGQTIGNS
                     LRRILLSSLMGASIVAVRMKGILHEFSTIPGVTEDVTDIILNLKEVRLRLNDGEQHTL
                     KVEAKGPATVLAKDITGSANVEILNPEQKIATLSRDAKLDMELIAKLGRGYVPAERNR
                     EEGAPVDTVFIDAIFSPVQKVNFTVTNARLGQHTDYDRVVFEVVTDGSVKPDDAVAYA
                     AKILQDQLSIFVNFQDEPQQERRDDRPSIPLNDNLFRSVDELEFSVRSQNCLQNADIK
                     YIGELVQKSEQEMLKTKNFGHKSLNEIKEILREMGLELGMKIDHFPPREEIESRRRAD
                     EKETA"
     gene            complement(57846..58472)
                     /gene="rpsD"
                     /locus_tag="VF182_00690"
     CDS             complement(57846..58472)
                     /gene="rpsD"
                     /locus_tag="VF182_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX6435613.1"
                     /translation="MARYTESVCRLCRRENLKLFLKGERCYTDKCAIERRNYPPGEHG
                     QGRPKFSEYSIQLREKQKVKRIYRLMESQFRRTFDQASRIKGITGETLLVFLERRLDN
                     VTYRLGFASSRAEARLLVRHGHILVNGKRLNIPSYQVRVGDVISVKEQSRQMARILTA
                     IEGVQRRGVPDWAELDRDNCSGRIRILPTRSDITMPINEKLIVELYSK"
     gene            complement(58494..58949)
                     /gene="rpsK"
                     /locus_tag="VF182_00695"
     CDS             complement(58494..58949)
                     /gene="rpsK"
                     /locus_tag="VF182_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX6435614.1"
                     /translation="MAKVEDTADEKQGKKTETPEDQKGADRAARKKRGRKNISEGVVH
                     IHSTFNNTIVTITDYQGNVISWSSAGSMGFKGSRKGTPFAAQQAADNAAKKAMDHGLR
                     TVQIFVRGPGAGRESALRALQSAGINISLIKDVTPIPHNGCRPPKRRRV"
     gene            complement(58970..59353)
                     /gene="rpsM"
                     /locus_tag="VF182_00700"
     CDS             complement(58970..59353)
                     /gene="rpsM"
                     /locus_tag="VF182_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX6435615.1"
                     /translation="MARLAGVDLPRNKRMEVALTYIYGIGRNLSREILNQAGISLDLK
                     SDDLADDDVVKIRQLIDQNYKVEGDLRRDVSANLRRHIEMGSYRGHRHRRNLPVHGQR
                     THTNARTRKGPRKTVAGKKTAPSKG"
     gene            complement(59380..59493)
                     /gene="rpmJ"
                     /locus_tag="VF182_00705"
     CDS             complement(59380..59493)
                     /gene="rpmJ"
                     /locus_tag="VF182_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX6435616.1"
                     /translation="MKVRASVKKICNKCKVIRRKGVVRVLCENSKHKQRQG"
     gene            complement(59507..59725)
                     /gene="infA"
                     /locus_tag="VF182_00710"
     CDS             complement(59507..59725)
                     /gene="infA"
                     /locus_tag="VF182_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX6435617.1"
                     /translation="MPKEDAIEVMGTVLETLPNAMFRVQLENGHRVLAHISGKMRMHY
                     IKILPGDKVKIELSPYDLARGRIIYRER"
     gene            complement(59757..60515)
                     /gene="map"
                     /locus_tag="VF182_00715"
     CDS             complement(59757..60515)
                     /gene="map"
                     /locus_tag="VF182_00715"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX6435618.1"
                     /translation="MISLKSAREIEIMRRANLIVAEVLQELKAKIAPGVTTLDLDRIA
                     EESTLKKNAIPAFKGYSVGGRVFPRCLCASINEEIVHGIPSNRTLKEGDIVGLDYGVI
                     YEGFYGDSAITVGVGKVSEEARRLMEITEKSLYEGINQLYEGKRLGDLGAAVQMTAEG
                     AGYSVVRAFVGHGIGKRLHEEPPVPNYGEPDRGLRFKEGMVLAIEPMVNIGGPEVEIK
                     ADGWTAVTKDGSLAAHFEHSVAVTKDGPYILSQL"
     gene            complement(60512..61174)
                     /locus_tag="VF182_00720"
     CDS             complement(60512..61174)
                     /locus_tag="VF182_00720"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX6435619.1"
                     /translation="MASVRVVLLGPPGAGKGTQAKLLRERFEACQISTGDILRKAVAD
                     KTPLGAEAESYLNRGELVPDDVIVRLVGERLKQRDCAQGFVLDGFPRTLPQAESLEEI
                     LKAMALPLDRVLSIQVPQQIIVQRLAGRRNCRDCGALYHMNFDPPRNDETCDRCNGRL
                     QQRDDDREETITTRLRVYETQTAPLVNYYGQRGNLRKIDGVGKVEDIQKRIIDALARP
                     AN"
     gene            complement(61178..62485)
                     /gene="secY"
                     /locus_tag="VF182_00725"
     CDS             complement(61178..62485)
                     /gene="secY"
                     /locus_tag="VF182_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX6435620.1"
                     /translation="MLSGFQNASRIPELRKRLLFTLAMLAVYRLGVHVPTPGIDRQAL
                     AAFFEQYKGTIFGLFNLFSGGALEQFSVFSLGIMPYISASIILQLLTVVFPYLERLSK
                     EGEAGRRKITQYTRYGTIVLAVIQGTMISLGLAGTQTGGQPIVLRADLSFHIMTVITL
                     ASGTAFIMWLGEQITERGVGNGISLIIFAGIIARLPSAITTTFQFIREGELNVLVALF
                     MLVLIVAVVGAIIFVERGQRRIPVQYAKRVVGRKMYGGQSSHLPLKVNTANVIPVIFA
                     SSILIFPATMAQFAQYPWVQAAATYLSPGQWLHDLVFALLIIFFAFFYTAVVFNPTDV
                     AENMKKFGGYIPGIRPGQKTADYIERVLSRITLSGAIYLCIVALLPTILVSQFNVPFY
                     FGGTALLIVVGVALDTVAQMESHLITRNYEGFMKRGRLRGRRG"
     gene            complement(62478..62930)
                     /gene="rplO"
                     /locus_tag="VF182_00730"
     CDS             complement(62478..62930)
                     /gene="rplO"
                     /locus_tag="VF182_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX6435621.1"
                     /translation="MKLDDLRPAPGSTKKQKRVGRGDGSGHGKTACRGHKGQRARAGG
                     GTRPGFEGGQMPLQRRLPKRGFNNPFKVEVAIVNLDQLEALSADSEINPELLSARGLV
                     HGKNRRIKILGDGALTKSLTIRAHSFSAKAKEKIEAAGGRAELITTHA"
     gene            complement(62932..63429)
                     /gene="rpsE"
                     /locus_tag="VF182_00735"
     CDS             complement(62932..63429)
                     /gene="rpsE"
                     /locus_tag="VF182_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX6435622.1"
                     /translation="MERIDPHGLELKEKVVHISRVAKVVKGGRRFSFSALIVVGDNRS
                     VVGCGMGKANEVPEAIRKGLEQAKKNLIRVPVMSGTIPFEVTGRFGAGYVILKPASDG
                     TGVIAGGGVRAVVEAAGIQNVLTKCLGSNNPHNMVKATVEALRDLRAPEEIAERRGKT
                     LAEIR"
     gene            complement(63455..63820)
                     /gene="rplR"
                     /locus_tag="VF182_00740"
     CDS             complement(63455..63820)
                     /gene="rplR"
                     /locus_tag="VF182_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX6435623.1"
                     /translation="MATKRKNIGRIRRQQRVRKKVLGTDARPRVCVYKSSRHIYAQVI
                     SDAKGVTLASASTLSAGLPDEIKSMKGVEAAKRVGLNLAKVCKEKNINRVVFDRNGFL
                     FHGRVKAVADGAREGGLEF"
     gene            complement(63839..64387)
                     /gene="rplF"
                     /locus_tag="VF182_00745"
     CDS             complement(63839..64387)
                     /gene="rplF"
                     /locus_tag="VF182_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX6435624.1"
                     /translation="MSRIGKLPITVPSGVKIAVDGDAVRLEGPKGKLTAAIPSGISVK
                     VDGTVVHVERGEEVRRIRALHGLTRKLIANMAEGVSKGFNRILDINGVGYRAEVKGQE
                     IHMTLGYSHPVVFPLPPGVTATIERQVIITLTGADRQLIGETAAKIRSLREPEPYKGK
                     GIKYREEVIKRKAGKAVGSAGK"
     gene            complement(64404..64808)
                     /gene="rpsH"
                     /locus_tag="VF182_00750"
     CDS             complement(64404..64808)
                     /gene="rpsH"
                     /locus_tag="VF182_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX6435625.1"
                     /translation="MGMTDPIADMLTRIRNASRARHQRVAVPWSRVKENIAKVFVEEG
                     YVKEYKKVKAAEGAGEELIIQLKFDRDNRPIILGLKRVSRPSLKVYVGAQQLTPIRKG
                     LGINVLSTSKGILVDREAQRANVGGEVLCSVW"
     gene            complement(64822..65007)
                     /locus_tag="VF182_00755"
     CDS             complement(64822..65007)
                     /locus_tag="VF182_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX6435626.1"
                     /translation="MAKKCLRIKAERPNKFKVREYNRCPLCGRSRAFYRRFRMCRLCL
                     RDHARKGHIPGLIKASW"
     gene            complement(65029..>65419)
                     /gene="rplE"
                     /locus_tag="VF182_00760"
     CDS             complement(65029..>65419)
                     /gene="rplE"
                     /locus_tag="VF182_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011434164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX6435627.1"
                     /translation="LDSTVAEITAIAGQKPVITRAKKAIANFKLREGVPIGCMVTLRR
                     DRMYEFLDRLIHVALPRVRDFKGVSDRSFDGRGNYSLGLREQIIFPEINPDKVEKVRG
                     MTVTITTTAKTDPEGRALLKQLGMPFG"
BASE COUNT        14961 a        18481 c        16963 g        15014 t
ORIGIN      
        1 acattatggc aacgaaaaaa gcgagatcca cgatcagacg ttatcctaac ccaagaagcg
       61 tttgcacttg cgctcataat ggggacggac cgcactctca gcatcgcgga acgaatggac
      121 acggagcgtg tctcgtcaaa ggatgtgatt gcaccaactt tacctggtct ggtttcctcc
      181 cggaataccg tcgagcgtac gaggaaaata gaactgacta aatggtgaac tcgtttgcta
      241 gtttgcggtc ggagcgtgaa agcaatcagt cccaacgcct caaatcgtta atgacggctg
      301 tcggccggga gactgcggaa ggggctttca cctcgtcttg gaaagaatta ttcgcccgag
      361 cgcggcgacc gctctctctg ccatattctc gccaccgtct cgagaagcga cgctacgcgc
      421 cgggttagat tgcgacgtca agcggagttg gcaaccggcg ctgccggcta ggacggttgt
      481 aactccaagg tcgcccgcat catccccaga gaatgcacaa gttccgcggt cttccgctcc
      541 agcgcctcct tggcatcgat cagttcgcgt tcaggcgcgt tgtcgcgccc ggaggatgct
      601 tctggcgttc tttagcgcaa ccgagcgtag cagtttttct tcgttgtcct ctgccgccat
      661 ttagcgctcc atcatctctg acgcgagtgc tgctgtgccg agcacgcggg ctcggatctt
      721 ggcaaacgcc gtgtcgcggc tggcggacgt gtcgtcgctg aaggcgaaaa cccgcagtca
      781 tcagtggcgc ccagttgttc aacggatatg tattccgcgg cctcaaggat ccgatcccgg
      841 acttcttccg gagttctatg cgtaaatcta tggttgcaat cacgccaata aaaatccgct
      901 gatccggttt catataatcg cgaataatct tctgaacatg agcgcggtcc ttttcaccgg
      961 ccaaagcgat gtaaaaatta cccacctgca gttcgaaaag gcttggcagc agctcggcgg
     1021 cactcgcgca taaagcacgg cagcatcgag atatgtgtcg gcatacctct tatagcgaaa
     1081 aggtcctgat gtgagtcggt gcatccggcg ggtgtggccc gtagcgaacg ggatcttgat
     1141 accatcgggt gcgataatgg gaaggtcgtg aacggagtac gtgcagaagt tgcgataact
     1201 ttctctgttc gcgcgtcgct gataaccggc gaccctgtcg cctcgaagcg ttcgatggca
     1261 tcccgaattg ccgcatcata gagctcatcc aacttgggat ccccattatc ccctgctgtc
     1321 gcgactgcct cgatcaactt aaccggtctt ggaatactgc caatcggctc ggttgggata
     1381 gtcatcctgg gcctctttat gagacacagc tatcgacgca cccggcccag aagtgttcgc
     1441 agcagcgcat ttcacgaatt ggcacggtac aagcctacca aattaaataa cgaaccttca
     1501 gttcggcatt ttataaggct taacgcaatc aactctcaat gatagttccg cagcgcgagt
     1561 gcagagacgg acacgagcta gggacctctc aagtcattgg gaaattaacg gctatctgat
     1621 agtgaaagcg aagcgcgaaa actaggtcgt cccatcccag ctgctggtat ggccgccgaa
     1681 agcgattagc ttgcttaccg ttttcccgga tcgtaaccta gaatcgatgc aagccaacgc
     1741 tccacttccg gaactttcat tccttttcgc gccgcatagg attccacctg atccttaccg
     1801 atggagctga cggcgaaata gcgtgattcc ggatgggcga aatagaggcc gcacaccgat
     1861 gcggcgggaa acatggcaaa actttcggta agcgcgatac cagtggcatc gctcactttg
     1921 agcaggtcaa aaatgatcgg cttctcagtg tgatccggtt gagccgggta accaggcgcc
     1981 ggacggatac cgcgatatct ttctgcaact aagtcctggg tgttcagctg ttcgtccttg
     2041 ccgaagcccc attcgcagcg cgcccgttta tgcaggtatt ccgcaaacgc ttcagccaaa
     2101 cgatcggcaa gggccttggc catgatggag ttatattgat cgtgatcctt ctcgaaacgc
     2161 tcaaccagtt cctgggttcc atggccggcg gttacggcaa aggcgccgat gcaatcgagc
     2221 actccgctgt cgcgcggagc gatgaagtca gcaagagcca gctgcggctt gccacgctga
     2281 ctttcttttt gctggcgaag ggtgtgaagc cgctttagtt cctgtttacg agtcgcatct
     2341 gtgtaaagca caatgtcgtc gccttcgctg ttggccgggt aaaaaccata aaccgcgtgc
     2401 gctgagaaca atttttgctc gacgatttcc gacaataatt ctcgcgcatt atcgaacaac
     2461 tcctgcgccg ccggtccgcg caccgggtcg ttcaacaaat cagggtagcg gccgcgcatc
     2521 tcccaggtat gaaaaaacgg cgaccaatcg ataaacggca ccagctctga caaaggaaaa
     2581 tctctaagcg tgcgcgcgcc gatgaattcc ggcaccggga taggaacttt atcccattga
     2641 gtgatgaacc ggcgccgagc cgcttcttca taggtcagca actgcgtgcc gacgcggttc
     2701 tcgaactgag tgcggatttc ctgttgctcg cgccgattct tttcacttag tgcctgtcgc
     2761 ccctcggggc tgatcaaggc gccgaccacc ggcactgccc gcgaggcatc gattacgtga
     2821 attgtttcat gcttgtacga tggcgcgatc tttactgccg tgtgtcgctt gctggtggtc
     2881 gctccgccga taagcagcgg aataaccaga ccgcgccggt ccatctcggc ggcaacgtgg
     2941 accatctcat ccagagacgg cgtgatcaaa ccgctcaagc cgatgatgtc cgccttttct
     3001 tctatcgccg tatcgataat cttatctgct ggaaccatga cgcccaaatc gatgacgtca
     3061 taattgttac agttgagcac tacgccaacg atgtttttgc cgatatcgtg gacgtcgcct
     3121 ttgaccgtcg cgaaaaccag cttgattcgt ttgtcgactt ctttggctga ttttttttct
     3181 tcttccagga aaggcgtaag gtacgcgacg gctttcttca tgacccgagc gctctttacc
     3241 acttgcggca gaaacatttt gccggatccg aacagatcgc cgacgacgtt catgccgccc
     3301 atgagcggcc cctcgatcac cgacaaaggc ttgtcatatt tgcgccgcgc ttcttccaca
     3361 tcctgatcga tgaagtcggt gagacctttc accagagcat gcgataaacg ctcttctacg
     3421 gtaccgccgc gccagccatc gtctttgacc gcggtcttac ctgcctgttt gtaggtctcg
     3481 gcaaaagtta ccaagcgttc agtggcatcg gggcgacgat ttaacaagac atcttccacc
     3541 aattcaagca ggtccttggg tatttcttca tagaccgcca acatgcccgg attgacgatt
     3601 cccatatcca ttccggcctt gatggcgtga tacaagaatg ccgaatgcat cgcttcacgg
     3661 actggattgt tgcctctgaa agagaaggaa atattgctca ccccgccgct gaccttggcc
     3721 agcggcaggt tctttttgat ccatcgcgtc gcctctatga aattgacggc gtagctgttg
     3781 tgctcttcga taccggtgcc gactgtcagt atgttcgggt cgaaaatgat gtcctgcggc
     3841 gggaagccaa cctgttcggt cagaatcttg tacgagcggg cgcaaacctc gatcttgcgc
     3901 tcataggtat cggcctgccc ccgttcgtcg aacgccatga cgattagcgc cgcgccatag
     3961 cgacgaaccc gtttggcctg cgcgataaac ttttcctcgc cttctttcat gctgagagag
     4021 ttgacgatgc ctttgccctg tagacattta agccccgctt cgatcacctc ccatctcgaa
     4081 ctgtcgacca tcactggcac tcgggcgatt tccgattcgc ccgcaatcaa ccggagaaac
     4141 ttctccatgg ccgctttgga atcgagcatg ccctcatcca tattgatgtc gatgatttgg
     4201 gcccctccct ccacctgctg gtgcgcgacg ctgacggcct tctcgtagtc gccggacaag
     4261 atcagcttgg cgaacaccgg cgatccggtg acgttggtcc gttcgccgat attaacgaag
     4321 ttggactcag gccggatggt aacactctcc aggccgctca agcgaagata tggctcgggc
     4381 gccggcggga cgtgcggcgg caatccttcc acggcttcgg caatacttgc gatgtgcgca
     4441 ggcgtagtgc cacagcaacc gccaacgacg ttcagccagc cgttctcggc ccattcgcgg
     4501 agttgcggcg caaggctctg cggggtctcc ggaaaaccgg tcggcaacag cggatcgggt
     4561 agaccggcat tagggtggct gctgatgtaa acgggcgcga tccgggataa ttcttcgatc
     4621 agcgggcgca tctctttggg acctaaagcg caattcatgc cgacgctcaa caagggtacg
     4681 tgggagatag agttccagaa agcttcgacg gtttgtcctg taacgccgcg gttgctgccg
     4741 ggctgaataa aagtgacgga tgccatcagg ggcaactcaa gctttcgctc ctcgaacaaa
     4801 gttgcgatgg cgaagaaagc cgctttcgca ttgagggtgt caaagatggt ttcgaccaag
     4861 aggatatcgg cgccgccgtc tatcaatccg cgagcctgat cataataagc ctgcaccaat
     4921 tcctcgtagg tcaccccgcg cgtcgccgcg ctgtgcacat ccgtcgagat tgaacaggtg
     4981 cgattggtcg ggccgattgc gcctgcgacg aatcgtggcc gggaaggatc cttcccaatc
     5041 atcgcttcga ccgccttctt tgccgcccgg gagcccgcga cattgatgtc gtaaacatcg
     5101 gcttccagct tgtaatccgc catggaaacc gaagtcgaat tgaaggtatt ggtttcgacg
     5161 atgtcggcgc ccgcttgcag atactgcaga tgaatttcag tgatcaattc gctctgagtc
     5221 agacagagca cgtcattaca acctcttaga tcgcgagaat ggtttgcgaa ccgttgaccg
     5281 cgaaatgcct cctcatcgag cctgtgcgcc tggatcatag tgcccatggc gccatcaaga
     5341 accagtattc tgtgggctag cagttcgcgc aatgtttttt cagtctcggt catcacttcg
     5401 ccttccttga gattggtagc tttgccaagc ctgcacgggc cacgaaaatc tccaccggtt
     5461 gattcccgtt gcgaaccatc ttttcaccgg cataagaacc cgtcagctca tactcgacgc
     5521 ctttcacccc gttagcacgg aatgcatctt caatcgcatc ccgatcaaag ccaagccaac
     5581 gatgcttcat ttgctcgcgc atccattctt gcttgtgttg caccaggtcg accaggataa
     5641 tcgatccaca tgggcgcacg atccgacaaa gttccgcaat cgctttttct ggatccgcta
     5701 gaaaatgcag caccatgacg cagaatgcgg catccacgct atgcgtcggg atcggcagct
     5761 tcaacgcatc gccgagacgc aagtcgacat ttgttacctt tttttggttg gccagttcac
     5821 gagcgcgacg taacatctct tgcgacagat cgacaccgat cactttcccg gcaaatcgcg
     5881 ccagttcaaa cgtgaggctt ccggtgccgc agccaacgtc ggccaacacc atgtttcggg
     5941 gcagcagctt ttcaatcgct aacgaggcga cccgatcgtc gaaataactt ttacgaattc
     6001 tttcccagtc gccggctacg gtttcgaaat agtcacgcga gcggctcagg cgccggcgaa
     6061 gacagttctc aagccgagcg cgatcgcctt tggcttgcgg caaatctcca atccgctcgc
     6121 gtaatgactt gaatagttcc tgggccggcg cgggcatatc gcggcgggcc gagaaataaa
     6181 cgttggtgcc ttcctttcgg tcctgaatga aacccgcttc gcgcagaatc gccagattgc
     6241 gcgacacgga agactgcacc gaagcgacga cttcctgcac ttccccgacg gtcaactctt
     6301 cttgggcaac ggcggcaaga acgcgcaagc gcaacggatc ccccaaagct ttgatagtgc
     6361 gaatgatatc catagctgcg gcttatagat atcgcaatat atagattatt agatattcac
     6421 gcaagacgat gcgataaaat ggcgcgaccg agccgctctg ccgtatacgt cggcgatcga
     6481 tcgtatcatt gaccgtaaag gagcgcatcg tgcgctccgg gagtacacgg cgggaaccgg
     6541 ccgtggagag gcataattcg atcgtagcta ggggcccttg cttaaactgg taagagcttc
     6601 gagagacaag agctatcgaa tcttctgagc gacaataccg gcgcgaaacg ccggctctcc
     6661 acggtcgctg tactgcccct cgcgatagca gagaacgcgc aagctacgaa agtgatccag
     6721 cagttcgttg tgatccagca gttcgttgtg atccagcaag taggcgggat ttttcggatg
     6781 gccaatcgcc tgctggtcga tcaggtaggt ttcaaaaata acatggccct ggggtctgag
     6841 cgctcttcgg aactgaggaa acaaagatcg ctgcagataa ttgaagttca cgatcagatc
     6901 gtaggcgccc gtgagaagtt caatgtgctc caagtcggcc tgcagccagg ttatctcaag
     6961 tgacttctcg tctgcgcgcc ggcgcgcctc ttcaagtgcg acggtcgaaa tgtcgatgcc
     7021 aactacgcta aaacccattt cggccagcaa aatcgcgtta cgaccttttc ccgccgcgac
     7081 atctagggct agtccgcggg agatgtgcca ggcatcactc tcgagaatct gccttagata
     7141 atccgatggc tgatcggcgc cagccgatga cgaatattga cggtcccatc tggtttgatc
     7201 atcagaagcc ataatagatt tgaagatgtt ccagtggtcc caatcgttgc aatggttcca
     7261 atggctcaag tacgaaaaga aggaaacgga tcaattttgt tcttggtatc gcaacgactg
     7321 caacgtgtcg aacctctgga acgaccggac cagtttcact atcaatacac tgcgcgcgcg
     7381 agagtaagca caaaaaccgc ccgcgcgccg ccctgtttca acacccggct gcactcgttc
     7441 acggtggcgc ccgaagtata cacgtcatcg actagcagca agcatttacc cttcactgcg
     7501 gtttttcccg acagggcaaa cgcgccgcgc atgtttttgc gtcggtcttc ttccggcaat
     7561 tgagtttgcg gcggcgtgtc catttttcgg taaagcgcaa agcaatctag cggaagattg
     7621 taggcgcgac caatctggcg cgccagcaac acggattgat tgaagccgcg ccagcgcagt
     7681 cgcctggggt gcagcggcac aggaataatc gtttctgcct tgcatgttag aagaaattct
     7741 tcacagccgt gcgccatcaa ccgtcccaaa ggcttgccta gggcgacttt gcgtccgtat
     7801 ttaaattttt gaatcacctg acggagcgga tgctctgtcg tttcttctct cggataacag
     7861 gcccaggagt gcgcccgggc aaactgtgga ttgcgcgaaa ggcacactcc gcaggcatga
     7921 tcatctccgc tcgaatctgg aaatggccgg ccacaaatcg tacagaacgg atgcgcaaca
     7981 agctgaattt gcggccaaca gatggaacag aaatattctc ggtcacgtcc ctggatcgag
     8041 ccgctgcacg cgcggcagcg cggaggataa agccaatcca ccagcgagtc cacgcgctcg
     8101 gcaaaagatg gatggcattg aggtttcatg gcgccgccac tctacaaaac gatctcacaa
     8161 tcaagcaagg ccgtcgtgaa agcagtgatt ggaatcctgt taagtatcaa tgatcagcga
     8221 gtgaccggtc atttctgaag gttgaggcaa ttcaagaagc tgcaacactg taggcgccac
     8281 atcgatcgcc gttccttcct tgatacggct tcgtttgcgt tcctcatcaa ccaggatgac
     8341 cggcactcgg ttgatcgtgt gggcggtgtg cggcattcct gtatcgtagt cgattagctg
     8401 ctcgatattg ccgtgatctg cggtgataac gactctcccc ttctggctca gcgccgcgtc
     8461 tactacttta ccgaggcatt cgtcgatgac ctcgcaggct tttaccgaag cctcgaaatt
     8521 acccgtgtgt ccgaccatat cggcattggc aaaattggca atcactaggt tgatatgttc
     8581 ctcggtaaac ttcttcacca gagcctcagt aacctgacgg gcactcatct cgggtttaaa
     8641 ctcatacgtc gggacatctt tcggcgaggg aatcagaatg cgttgttcgc cggggaattc
     8701 tttctcttcg ccgccattga agaagtacgt cacgtgggcg tatttctctg tttcggcgat
     8761 ccgtagctgc ttgagcccgt gctggctggc aacctcgccg agaatctttc gcaattcccg
     8821 gggtggatag gctaccggca ccctgaacgt ctcgtcgtat tgggtcatag ttgtgtacgt
     8881 cgcgagtttc acacatcgcg tgcgggggaa ctccttaaag tcagcatcga tcagcgcccg
     8941 ggtgagctca cgtgcgcgat cggcgcggaa attgaagaat atgacaccgt cgccgtccct
     9001 gatcagtcct tcgggaaccg cgctccggat caccgtgggt aaaacgaatt cgtcggttac
     9061 gccctctttg tagctgttac gaatagcttc cagcggcgaa tccgcgagct tgccggcgcc
     9121 ttcggtaaga cagagataag ccttttctac tcggtcccaa cgtttatccc gatccatcgc
     9181 gtaataacgg ccaatgagag tggcgatctt tacatccggc catgctttca acttctcatt
     9241 cagatcaaga atgaactgct cggcgctgtt gggcggcgtg tcgcgcccgt caaggaagag
     9301 atgcagatat acgcgtccga gcttttcgca tcgggccata tcgatgagcg cttccatatg
     9361 acgctggtgg ctgtgaacgc ccccatcgcc gaggaggccc atcaagtgga ggcgtccagc
     9421 gccggctttg gagtttttta aggcatccaa gatgattgga ttattgccaa aattgccctc
     9481 atcgatgtcc ttgtgaatca gcgtcaagtc ttgataaacg atccggccgg cgccgagaat
     9541 catatgccct acttcggagt tgcccatctg gccctcggga agccccacat ccagaccaga
     9601 catagaaagg ctcgagcttg gatagttccg caacaacctg tccatattcg gcatcgacgc
     9661 attagcgatt gcgttgcctt ctttttttgg gttgatgcca aagccgtcta agacgacgag
     9721 aataactggc tgattggtcg ccacaatatt tcctttcatc tctcccgccg cttgctcgcc
     9781 atcgcggcaa gcggcagccg aggggagctt ggaagagtta cggatgctgc tcagcggcga
     9841 tctgaaactg gtgaaccaga gtcgagtaag gttcgggcaa ttggactttc ggcgcgtcgg
     9901 cccaaagtcc gtctaggtca tagaactcgc gcacaggctg gtagaaaaca tgaacgatca
     9961 cgtcggcaaa atccatcaac acccaatgcc ctcggcttgt accctcgacg gaaacgacgg
    10021 caatctccgc ttcacgcgca gcctcgtgaa tcccctgggc gatactttgg acctgacggt
    10081 cggatcggcc tgagcaaatg acaaagtaat cagcgatcga agtgaattct ctaacttcta
    10141 acaagacaag ttgtgatgcc ttcttatcca atgccgcgcc aaccaaggtc agggctctct
    10201 ccttcgaagt tttttctgcc ggcttgttca tttttttctt ttgtacaacc catgattttc
    10261 gatgtaggcc tcgacgctgg aaggcaccag atagcggatc gacttgccgt accgtacccg
    10321 ttcgcgaata tccgaggccg atacggcaaa gtcggtgagt tttaaaaaat ggagcgacgt
    10381 accacaccta tgcgtgtaaa tttttctttt aaaatcataa caaaacagcc tacgcacagc
    10441 aatggaagtt ccccgcagcg gactggcata tccattccct ggacgggacg ttacaatgaa
    10501 attacaaaag ggaaagatct tttcaaaatc cttccaggtg ccgatttcgg caaatgcatc
    10561 caaaccgata ataaaataga gcgagtcgcc cttacgttga tcttcggcga agtatcggat
    10621 ggtgtcgatc gagtaggaaa cgcccggcct ggcaatttcg acagtggaaa ccgtgaagcg
    10681 ggaattttta gctaccgcca agcgcaccat cgcgagccga tggcgcgccg gagtccgggt
    10741 ttgccctctc ttgtgcggtg gaatcgaagc gggaatgaaa aacacgcgat cgagatcgaa
    10801 ggcttctctc acctcctccg cgctgcgcag atggccccaa tggatcggat cgaaggtgcc
    10861 gccgaagagg ccgatcctca tggaaactcg atatcgcctt tcccggaagc ggcccaagaa
    10921 attttttgtg caagcggcgc aaagatttgg caatcacaag cgtctcgatg actgatttcg
    10981 aacaacctgt ttggcgctgg actaggcaaa taaatgattt tcatgacgat cattcgcgaa
    11041 tctgcccatc cccgaacaca atgaacttgg ttgtcgtcag ttcctctaaa cccatcggtc
    11101 caaaagcgtg aatcttgctc gtactgatac cgatctcggc gccgagaccg agctcgccgc
    11161 cgtcattgaa gcgagtcgaa gcattgacca tcaccgccga agagttgacc cggtcgatga
    11221 actctcggga tcttcgataa ttggtcgtaa tgatcgattc ggtatgttcc gagccgtact
    11281 gttcaatgtg cgcaatcgcc tggtccatgt ccttgacgat tcggacggca agaatcagat
    11341 caagatactc agtcgtccaa tcctcttcgt tagccttctt gatgtcggga accaacgcgc
    11401 tggttttatc gcagccgcga atctctaccc ccgcagcctg atattttgcg atcagcgccg
    11461 gcagaaattt cgatgcgacg gcttggtgga ccaacagagt ctccatcgag ttacaaaccg
    11521 atggccgttg aaccttggcg tttagacaga tccgttcggc catatcgagc tcggcatcga
    11581 catcaacata gatatgacaa acgcctttgt agtgcttgat gaccggtact ttagaattcg
    11641 ccacaaccgc gcgaatcagt tcctcgccac ctcgcggaat aatcagatcg atgtacgact
    11701 ccaattgcag caactcgtga accaacgcac gatctgtgga ttcgaccact tggatagcat
    11761 gtttcggcac cgccgttacg ccgcaagcgg tttgcagcac tttgccgatt gcctggtttg
    11821 agttatgcgc ctcacttcca cccctcagga taaccgcgtt tccggacttc aaacacagcg
    11881 ccgcggcatc ggcggttaca ttgggacggg cctcatagat gatcccgata acacctaaag
    11941 gaatgcgcat ctttccgact tgcaagccat tggggcggcg cgacatctta actatttcgc
    12001 gcaccggatc cggcaacgcc gcaatttcgc gcagaccttt ggccatcgat tgtacgcggc
    12061 tcgaagtcag cgcaattcga tccagaaccg cgccggtgat ctttgctttc tttgcagcat
    12121 ttagatcatg ctgattctca tcaattaaaa atgcgctttg agcctcgagt ccgtccgcca
    12181 tgagttgcag cgcctgattt ttatcctcgg aggaaagcac cgcgaggcgg cgcgccgcag
    12241 ccttggcttg ccggccgagc tggattgctt gttcggcgaa tgaggcattc gccaatgtgc
    12301 ttttcttcac agcaacacca gatcgtcgcg atggataatc tcgtcaaatg gcttgtagcc
    12361 gagagcttgt tcaatcctgc ttgtatgcaa tcctttgatt tgattcaact ccaaagcgct
    12421 gtaattgatc aaaccgcggg caaactcccg cccgtcaaga tccacgcaac gcacgcactc
    12481 accgacccca aaagagccgc gcacctctct cactccggaa ggcagcagac tggtcttctt
    12541 tcgcaccaga gcttcatgag ctcccgaatc gacgacgacc tcacctgcgg gctttaaatt
    12601 gaaagcaatc caatgcttgc gattggttaa gcgattacta tcgggaagga taacggttcc
    12661 gacttcttcg ttcgggtcga atacgccggc gatcgaaccc gcgatcaatc cgctggttat
    12721 caccgtgggg atgccggcgg cggctgcttc ttcggcggcg gcaattttgg tcgccatgcc
    12781 gccggtgcca aagacgcttc tgctcgagcc gatcactttg gatttgatat ctttgatgtc
    12841 ttcaatcagc ggaatgatta ccgcattgga atctagtcgc ggatcacggt catacactcc
    12901 tgcgacatca cttagaataa gcaatagatc ggcttcgagc agcgtcgcca ctagcgccga
    12961 gagatgatcg ttatcgccaa acttcatttc ctccacggca actgtgtcgt tttcattgac
    13021 gatggagatg atcgacgatg ccagcaacat ttgaaaggta tgtttggcat taagataccg
    13081 ttgccgattg gcgagatctt cgtgggtgag cagaacctgc gcgacgttct tgctgaactt
    13141 gccgaaggcg cgctcgtata acgccatcaa cctgatttgt ccaacggctg caagcgcctg
    13201 tttctcggga atgctctgcg gccgctcttt gcgacggagc cgagtcatgc cggctgccac
    13261 ggccccggaa ctgaccacga cgatctcttt gccttgatca tggagtgccg ccagctcgga
    13321 tactaaggct tcgaggcggg cttcctctat gccggttgag gaagaaagga tctggctgcc
    13381 gatcttgacc acaacccggc gcgcccggcg cacaattgat ttcttatgat cgcgctgcgt
    13441 caccggcttc ttcttcctta acgcgcttgg ctcgctccac atgcatccca accttttgca
    13501 tcagcgtccg tactccctcg ccggtcacgg cggagattag atacagcggc cgatatggat
    13561 ccggcaattt ttcttcgatc aattttgcgt tctcccttgc ctcgggaata tcgattttgt
    13621 ttgcgacgac gacttgtggt tttctcgcca gatcctcgtc gaagagttcc aattcccgat
    13681 tgatggtgga ccagtcggct agcggccttt tcggatcaat tcgtgaccca tcgagcaaat
    13741 gaatcaacac actcgtgcgg gtcacatgtc ttaaaaactt gtcccccaag ccatgtccgt
    13801 ggtgggcgcc ttcgatcaat cccggaatat cggcaaccac gaaactcttc ccttcattgt
    13861 agctgaccac accgagattg ggcaccaggg tcgtaaacgg gtagtcggca attttcggcc
    13921 gcactgcaga aataaccgag atcaaagtgg acttaccggc attgggaact ccaataattc
    13981 caacgtcggc caatagccgc aattcgatct ccaactcgcg atcctcacca ggttcacccg
    14041 gctgggcgaa ccgcgggcta cgattggtcg atgaaacgaa atgcgcattg cctttgccgc
    14101 ctcgcccgcc agcggcgagc acaatacggt cgccggcctt atccagatca ccgatagtct
    14161 cgccggtccg ggtatcgcgg acaatcgttc ccaccggaac tttcacgata cagtcatcgc
    14221 cgcgacgtcc atgacagtcg ctgcccatcc cgtgctggcc gcgaccagcc ttgcactcct
    14281 tatgatagcg tagatccagc aaagtcgtga gctgtggatc cgcaaccgca atgacatcgc
    14341 caccggtccc gccattgcca ccgtcggggc cgccgcgact gatgaacttt tcgcgatgaa
    14401 agctgacgca accacgtccg ccatcgccgc cgatgacccg tatttttgtc tcgtcgatga
    14461 atttcatcac gctgaaagta aacgtaagcc gcagtttaat cagtccggtc tacatcaata
    14521 cgttcgcttt tctcctcccc gcttctactt tacccattct caaatcactg caccatgttt
    14581 atcatgggca aaaaaaaagc ccctttcggg gctttcggtt caatgcaggc gatttcctac
    14641 tgcacaggca gcacgtttac ccgtttgcgc gaacggtcga gccggtcgaa gttcaccacg
    14701 ccatcgatca acgcaaacaa agtaaagtcc cggcccatgc ccacgtttcg gcccgggtga
    14761 atgcgggtgc cgagttggcg tataatgata ttaccggcac gcacggtttc accgccaaac
    14821 actttgacac cgcgtcgttg ccctttgcta tcgcgcccgt tacgggaact cccaccagct
    14881 tttttgtgcg ccatatcgtg tcctactctc tagatcttct aaccatcgat tccggtgatc
    14941 ttaagcgtgg tctgatgctg ccgatgacct cgctggcgac tataactctt acgccgcttc
    15001 tttttgaaaa ccagaacctt cttcgctttg gcttggctga caatctcgcc ggttacagag
    15061 gcgttctcca gcagcggcga accgattctg acgtcgccgt tacctccgat aaacagcaca
    15121 tcaccgagag ttatcttttc gccgacttcc ccagtaagct tctcaatctt tacgagatcc
    15181 ccttcagata cccggtactg cttcccgcca gttttgatga ctgcatattt catgatctta
    15241 ccaaaagatt tttgtaacgg agattccagc caaagtcaat aatatccggt cacgaggacg
    15301 cttgcggtag cccttgggat aacgcgcacc aaaaactttt ttagacccta gttgacaaag
    15361 gctcgacagt taaatataca tcgcaatgcg atagtatcgt aacacgatat tgtttgatga
    15421 tggtgatccg tgcccgattt taaagcaatg gcggaactac ggtatcaaat acgtaagttc
    15481 ctgagattca gcgaaaatgc cgcacgccaa gccgggatcg aaccgcaaca gcaccaattg
    15541 cttttggcca ttaggggatt accggatgcg gcaagtccga ctatcggagt gctggccgag
    15601 agaatgcagc tgcagcacca tagcacggtg gaacttgtgg accggctggt agaccgaaat
    15661 ttcttgtgcc gattacgatc gaccaacgac cgccggcagg tcttggtcaa gctaacgcac
    15721 gatggagaaa attttctcga gaagctttca ctgcaccact tggaagaact tcagtcagcc
    15781 ggaccgacct tcgtcaaggt ccttaatagc ctgctcgccg atagtctcat cgacaatccc
    15841 aactccgcct ctgaagtagc gaaggaaacc gtgaccgata aaccacgaaa ggatagaaac
    15901 catggctgat caatcggctg aagaactatt gctcgacgta agggttgccg aagcgtcaag
    15961 actttacaag ttctatttaa ataacgaaca gaaaatactc ggcacagcga gtgtggtgac
    16021 gttcttagtg gcatgggagc tcgttggaaa tgttcttcaa ttgatcaatc ccatgttcat
    16081 gagcgcgcct tcgctgatct tcaaggccgc gatcgacatg ttttcctccg gcgagatata
    16141 caacgatctc tatgtcagcg gtatcgaact cttctgggga tatgttctct cggctgtcgt
    16201 cgccgtcccg tttggcatca tggtgggctg gtacaagaga gcgagctata tctttgaccc
    16261 tttcatcaat gccatgaacg cgacccctcg agtggcgctt ttgcctctgg tgattatctg
    16321 gttaggaatc ggcatacttt cgaaggtcgg cattatcttt ctaggcgccg ttttcccgat
    16381 cttgatcaat acccgcgatg gagtcaaaac cactccgtat aaccttctca ctgcggccag
    16441 gagttttggc gcttcggagt ggatgctgtt caaaacagtg gtgttgcctt cgacaattcc
    16501 ttttatcctg accggtatgc gactcgggct gggccgggcc atcgtcggag tcatggtggg
    16561 cgagctttac gcggctaccg ccggcattgg ctttatgatc accgttgccg gcgccacttt
    16621 tcagaccgac aaggtcttcg tcggtgtgct gatcttcgcc ttgaccggta tgatcggaat
    16681 ggaggtactc acccggatcg agcggcgttt cgacaagtgg cgccccaaag tgggagcgca
    16741 ggagatctag atccaaactt aggtatttgg ttcgcgtgct tcaaaaggcc cctctagcga
    16801 ggggcctttt ttttgggacc ggggatgtgc tcccgccagg cctcttgatg cccgtccagc
    16861 cgtttgccat tgtgaaagct ggattctgac tcacgaaagg acctcacatg cgcaatctca
    16921 catcaggctt ttatcttctt cgttcgtcca tgatccttct agtcctaacc gcaaccgggt
    16981 gcggttataa taccctccag agtctggacg aagaagtgaa aggcgcctgg ggagaagttc
    17041 agaatcagta tcagcgccgc gccgatctcg tgcccaacct ggtcgcgacc gttaaaggag
    17101 cggcgaaatt tgaacaggat accctgacac aggtgatcga agcccgaagt aacgcgacag
    17161 cgaccaagat cgatccagag acgctcaaca atcctgaagc attcaaaaag ttcgagcagg
    17221 cgcaaaacca gctctctagc gcgttatctc gtttactcgt ggtggtggaa cgatatccag
    17281 agctgaaggc aaaccaaaat ttccaaaact tgcaagcgca gctggaaggc accgaaaacc
    17341 ggattgcggt ggctcgcaaa cgttatgtcg aacgcgtggc tcaatacaat actgctgtgc
    17401 gttcgtttcc cactaatctc acggcaaagt tcttgggctt aaatgttcgg ccgtcgttca
    17461 gcgccgacga agccgcgcaa aagccgccac aggtaaaatt ctaggccgta cagcgaatca
    17521 gtaccttgcg ccaagatcct catccacgga taccgcgacg cggtcatttg tccacgccgc
    17581 ataagtttgc ttttttcctc ttcttgcttt gcctgctatc gactcgagcc tatccgctgg
    17641 atgtgccgcc gctgcgcggg cgcgtgaacg attacgccaa agttattaca gcggatcaaa
    17701 ctcgcaagtt ggaagatcaa cttgcgcaat ttgaacgcga tagcggtcat caggtggcag
    17761 ttttaacaat cccatcgctg gagggcgact cgcttgagga cttcagtatt cgcgttgccg
    17821 aaaattggaa aatcggcaag aaaggcttcg acaacggtgt cattctgatt gtcgtgccga
    17881 aagatcgccg actgcgcctg gaggtcggat acggattgga gggcgtgttg cccgacgcca
    17941 tcgcgaacca gatcatcagt caatacatca taccacgttt ccgcgccggc gatttctcga
    18001 gcggcattgt cgcaggtacg gacgctgtgc tgagggtcat caagaacgaa ccgctcccgg
    18061 aagctgaccg gcagaaaccg gcgagacctt ctggatcagg gttcgggtcg ctcactggct
    18121 tgctgctgct cctggggttc atagctgtca tgtcgatggc ctccaacacc aaccgccgaa
    18181 gacaaggctt atggacaacc cgcggcaggc gccccccgcc gatcttttgg ggtggcggcg
    18241 gttttggcgg aggtggattc gggggcggcg gaggctttgg cggtggcgga tttagtggtg
    18301 gtgggggcgg attcggcggt ggcggagctt ccggaagttg gtgatgatgc gtccagacaa
    18361 gtttcttact gaagcagagc gggaacgcat tcaccaggcg gtgatcgcgg cggagtccaa
    18421 gacttccggc gaaattgtgc cgatggttgt cacctccagc gcgcgctaca atgaaatcga
    18481 actgtacggg atcgtcagcg ggatgtttct cggaatgctt ttaggctggc tctggcacga
    18541 tccctgggga tcctatcgtc tcaatgtctg gccggccgtc ggcgccactc tcggcttttt
    18601 gatcgctcgc atccctgcag tgaagcgcgg gctgacatcg agcagtcgag tcgaagaagc
    18661 tgtaaacaat cgttgtatgg ctgcattcac cgagcacggc ctgcattaca cacgcgacca
    18721 caccggcatc ttaatcctgg tgtcgttatt ggagcatcga gtcgaagttc tagccgaccg
    18781 cggaatcaac gagaaggttc cgccgggcac gtggaacgaa attgtcgaga ttctcactgc
    18841 aggcttgaag tcacgccatg ccggcgatgc tttctgcaaa gctgtcgagc gttgcggcga
    18901 gattctagcc agtcactttc cgcgccaagc cgacgatcgc gacgaacttc ctaataggct
    18961 cgttagcgat tgatgtgctt gcggatagtt actttcgaac caagcgcacg tccaccgcgg
    19021 ctgcgccgcg accctgcgcc cgcaccatca acggattaat ctctaaatcg gctagatgat
    19081 ttcgatgcgc ggcaaaaata tccgacaagc cgatcatcgc tttcaccaaa gcgtcgatat
    19141 cgcgcgggcc ttggccgcga accccctgaa gcaccttgga gccgcgcagc tcttgtaaca
    19201 tccggcgcgc ttctgcttca tcgaccggca gcagccttat cgcgacgtct ttcaagactt
    19261 cgacgagaat gccgcccaag cccaccatca aaaacggacc gtattgcgga tccatgcgcg
    19321 cgcccaacag cacctccgtg ccctgcacca tttcctgaac cagcaggtgg cctttgccta
    19381 gcgttttgga tagaagcttc tgtccttcag cgaccacttc ctcgggactc ttcagaccca
    19441 ataccacggc acccgattcg gtcttatgaa cgatttccgc cgcgaccaac ttcaacgcca
    19501 ccggaaaccc aatctctttc gacttcaatc ccgcttcagc cgcggttcct accagaaatt
    19561 gctttggcag tccgatgccg cagctctgca agagggcatt gagtttgtcg ccttccaact
    19621 cgtcccgatt gccattggcc tccggcagag tcggcacccc gagccgtttg cgttcgccgt
    19681 ataaacccaa gcccgcgagc gctcgcaacg tcggtttgat cgcctgcaga aatggaatcc
    19741 cagcttgttc ctgaaacgtt cgactttcgt cggtacagct ataggtcgaa cgggcgaaag
    19801 cgaggatcgg cttgtcagtg gcggcggcaa gatttttgaa caaagtcgct tccctcttct
    19861 caggaaaacg cggcaactcg ccgtgaatcg caagtaaatc gacagcatcg tcctccgcgt
    19921 gcagcttgac gatattgatg aagccctgct catcgccaaa tccggcaacg ccgcactcga
    19981 gcggattttc gacggcgagc tccggcggga tcagcggacg cacggcctct ttagtcttgt
    20041 cactcaattg ggctagattg gtgttgagtt cttcggcgtg atcgcagatc aggcctttca
    20101 tgccacccga gttgaccacg atcgcggcgc gactgccgcg aggataacgg ccggccatga
    20161 acgctagtgt catctccgtg agatcttcca gagtcgggca acggaccagg ccgagccggt
    20221 tgcataccgc atcgaatact tcatcggctc ccgccaatgc tccggtgtgc gagatggcct
    20281 gccgcttgcc catttccgag cgcccgagct taacgacgag aatcggcttg ttcttgttaa
    20341 gagcctcatg cgccagagcc atgaattcgt tggggcggcg gatgccctcc gccatcaata
    20401 agataacctt ggtccctgga tcgtccacca tgaacgagag atagtccacc agatcgaggc
    20461 tgatttcatt gccgctggaa acggcataag taaaaccgat gccgcgctcg gtggcgcctt
    20521 taatccaatt acccagggag ccgccgctct gaaagatcag acccacggga cctttcttta
    20581 gcaatggcgt tgaagtagga aatgtccaga ggccttcgct taaagagtac gagcccatgc
    20641 aatttggccc gcaaatgacc aaaccggtgc gctcgcacaa atccttcatt gcttgggcgc
    20701 gttccttgcc cttgggatcg tcgccttcgc caaaaccggc agtataaatc gtggctgatt
    20761 tggtgccgag cttggttgct tcttccaaag tgcccaacac cgctcgagtc ggaatcagaa
    20821 tcagcagttc ctcgggaacc tcgggcagcg ccgcgagatt gggataacaa ggataatttt
    20881 caatctcctg atagttggga ttgatcaaaa agatctttcc cgtgtaagcc ttcttcaggt
    20941 tgcgaaaaat tcccatcggc caacgccctt tcggtgatgc gccgacgata gctactgatt
    21001 tggcagagag aacattctcg accggcctgc gctggaatga agtcatacga ttccttgcta
    21061 gcttaaatta gattttccta tctcacgttc taaacgttgc tgcaacacag gtgaagcgat
    21121 agcgctctct caaccgctca tgagcggaag gaagcactgc gattccggcg ctaataggta
    21181 aaagcctggt ttttcaaaaa cgcatcggcg tcatccacgc taaagaaaag cagccgaact
    21241 tgttcgagcg aagcattaga tccaagaaat tcttcgatcg tctcggatga caccttggct
    21301 gcttcgctga gcggataacc gtagataccc gtcgataccg cggggaaggc gatagtcttg
    21361 agcccgtgct cgaccgccag acggagacag tttcggtagc atgaagccag caatgctgcc
    21421 ttctcgccgc ctcctcttcc gtaaaccggc ccgaccgtat gaatcacatg cttagcaggc
    21481 attctgcccg ccgtggtcat aaccacctct ccggtcggca gcccttgggg aaattccttg
    21541 gcccggattt ctttgcactg cttcaatatc tcaggaccgc ctgctcgatg aatcgcgcca
    21601 tcgacgccgc caccgcccat taaagtgccg ttggcggcat tgacgatcgc atcgacctgt
    21661 tccttggtga tgtcgccgat cttgaccgca attcgaccgt taagaaaaat ttttgaaatc
    21721 tccattaatc tttcccgcgt atcccctcca tcgccgagca gctaaacgaa atttttccgg
    21781 tctggaattt aggccaaaag acggcgacag aagcgcttac cagccgcggt ttgtcccacc
    21841 aagcttcgac gatcaatccc gtggcgcggg cgtgttccgc aacacttgtc ccggataggt
    21901 acctgtcacg gcgccgttct cgatgacaac ttccccattg accagcgtgt gatgcacacc
    21961 ggtggctcgt tggatcattc gcctctcgtt gcccgggaga tcctggacat attcgggcgc
    22021 catcgtatcg acggtatggg gatcgaacac cgtgatatcg gcggcaagtc cggatctcaa
    22081 caagccgcga tcgcgcaagc cgaatatcga cgccacctgg aaggttagct tcgagatcgc
    22141 ttcctccaag ctcataattc cgttgtcgcg cacccaccga cccaagaatg tggtgcagta
    22201 gccgaacctc gcatcatagc ccatgtgcgc gccggcgtcg gattgaccaa tgatgacgaa
    22261 tggatttttg aggatctcac agactgcgtt ggggtctcct tgcgtggtta cgtgcgagaa
    22321 gcgcgtttcg agattttctt ccaatgcgat atccaggaac acatccagcg ggtcttttcc
    22381 ccggctccgc gcaatttcct cgagagttcg accttgcaga gatttatgct tcggcagttt
    22441 cgcttcgtcg acatagatga cgtcccaacg cttggggaaa actgatggct ctggcgagtt
    22501 tacttcagcg cgcatttttt gccgcgcttc ggcgtctgca aaaatcgtct gcctttgctc
    22561 gaccggcata aacatcgcgg cgcgccaggt tggaaattca tcgaagcatt gcgcatcgag
    22621 cagagtataa cggttctcga acggtttggc ctgagtcatc gcatagattg gcaatccctc
    22681 ttcacgaaaa actctggcgg tggattcaag cattcctttc caatgatccg gccgggtcca
    22741 actatggctg atgctctgcc acaataccgg gcggccggtg gcgcgcgcta gttccgatgc
    22801 ataggcaatt cgtccgcgcg catccacacc accaccgctc aactgaacca ccccggtatt
    22861 catatcgccg agaacttcca acaaagccaa gagttcttga tgggacgcta cccggctagc
    22921 taccggcttg ccgtcttcgc gcatgtggcg cgggttctga ttggtcgaga agcccaccgc
    22981 gccggcggcg atgccctcgc gcaccaactg ctgcatacgg gcgatttcct ccggtgtcgc
    23041 ttctcgctcg accgcatctt ctcccatgac gtagtgccgc acggcgatgt ggccgatcag
    23101 tgcggcgaca ttgatgccaa gatgggagcc aagcgcatcc aggtattcgc cggtggatac
    23161 ccacttccat ttgatcccct gttcgagaac cttacggcta atggcctcga cccggacaaa
    23221 acttttaatc accgcctcgc ggccatcggg cttgcatggc gccaggctca ggccgcaatt
    23281 tcccaccacc gctgaggtga cgccgtgaaa acacgacgac gtgcctaatg gatcccatag
    23341 caactgcgcg tcaagatgtg tatgagggtc gataaatcca ggcgtcaccg ccaggccttc
    23401 gacattcaac gtacgcctgg ccggcccatc aagacggccg attgcggcga tctttcctcc
    23461 cgccacagcg acatcgccgc taaagctggg catgccagac ccgtcataaa ttcttccacc
    23521 cttgattagg agatcgaaca tggtgtctcc ttcaatatca cagccggtag agacattaat
    23581 tactcgaggg cagcgtcagt gattcatccg caatagccca ggagcccctc aaagggcaga
    23641 gtcaacggtt tgtgcgaaac ggcctaagct catctcctat gccccaggca cgagcccgtt
    23701 ctctttcaat aggggcaaaa gctgatcctg actatgaatc agcatgttcg gtttggccgc
    23761 catcaatgtt tcttcgtcgt tgtaggagta tccgttagag agcgcaatcg tgacgacttc
    23821 gacgtggttg ccggcgactt tacatccatc gacgttgttt ccggcgcgga tgtaggatgc
    23881 ggtgccactg agcaatatca atctcaagct cggcagtttg tgttctctaa acaagtagta
    23941 aagcccctgt tctttggcgt agtaggtctc atcgcgatcg acgatctcag caaagtattt
    24001 gccaatgcca aaagtctcca tcgcgtggtg ttgatagcgc cttccgcccg tagtcaatag
    24061 cacattgcgc agcccggcag acttagtact tttcagcgac tcctcgattc cgggaacgaa
    24121 gttgggaggt ttggtgtcga agctgtgctt ccgagcgatc tccagcgcct gggtgcgtat
    24181 ctccaaacgc tttttttcat tgaagatcag attggtgtcc tctttgtact catcggcgaa
    24241 aaccaaaggg atcaaaaaca aatcaaacgg cccgtcggca atcttacgca aacgttcaaa
    24301 cggcagcgga tgcagtccgt acttgggcag aatctccgtg gtaattgtca gaaccctttg
    24361 aatgtcggtc tccaccgtgt cggacatcgt tcgaaccaaa aaggtctcgg ttcccatgga
    24421 atgcctccag accagccgat actattcgaa gccaactgag ctggcaagcg agtttaccgt
    24481 gttcgtatta ccatcggaaa aagcagcgcc acatggcgac ataggagaac gcgcagctca
    24541 tccctgaatg gcaactattt tcctagccgg gactttcttc gaagggaagc cggtatctgc
    24601 gttcggagtt gcattttttg caactaggca caagattgcc tttggtggat ttaccgcctc
    24661 gaatgatcgg cacgagatga tccatggtca aaattgccgg cgggaatttg ccgccgcaat
    24721 agtggcagag gccaagggct cgcttgcgct tccaccactg gctcgcacgc agctctcgag
    24781 cctttcggcg ctcgcggcga atttcttctt cactgaccga gactagaaac gaagtcggtt
    24841 ccatgcggat gataaaacta cctgtaccat cgtttaatat tcacgaaata gacggcaaaa
    24901 gcgattgcgg ccagaaaata gccggccgcc tgaaagagtg cccagccatc gccggattgt
    24961 agatatcgcg ataactgcca gaatgaaaag ccgaagaaga aaatgatcga ggtggcaatc
    25021 agtattcgat gggcggtaat caatctcatg ggattagtga aacaccacgc tcgcagcaac
    25081 tgcaaggaag cggattgaac attgcttgcc tcgatcagga tacatcgtat gcacttggaa
    25141 cgttattatc aggagaaaat catgagtcga gaaatagtcc aaaccaatga agctccaaaa
    25201 gcaatcggcc cttatgccca ggcgattaaa gccaacggtt ttgtctacac cgccgggcaa
    25261 atcgctattc gccctgatag cggcgagctg gtcgacggcg gcatcgcggc gcaaacgcgc
    25321 caagtctttg ccaatctcgc cgcggtttta aaagcggcgg gaacatcgat ggatcaggtg
    25381 gtcaaggcaa cggtattcct gcgcgacatg accgattttg ccgcaatgaa tgaagtctat
    25441 gccgagcatc tcggtaaggc tactccagcc aggtcaacgg tagccgtgac ggaactacct
    25501 cggggagcgc tgatcgagat cgatctgatt gcagaggtgt agggcgagaa cgatctgcta
    25561 agtgcgcgag accatctgca ggagtttctg tagcaccgtc tccacaatgt tagtcaaaga
    25621 atcagatccg gcttcgatgg tttcttcgct aggcgggtat gcgaacatcc ccttggtgcc
    25681 ggtgaccacc caggattttt cgtcgccatc ggtttcgggt agtttcttgc cggttgtctt
    25741 ggcaccgata tttttgctgc gaactttttc ctggtggttc ttgtagcgtg tgaactgccc
    25801 gcgtttaagc cagatcccgt cgcacttgaa acaacgtcgg aactgcacat cggctcccag
    25861 cgaaggttca ttgatcgccg acattcgcgc ggtacagcgc gggcaaaaaa tttccttttg
    25921 caaaaccagc ggcaaccgga ggagtttctc gtcggcgggt tccagacgcg ccgcttcatc
    25981 ggcttggatc ggaaagagtt cccatttgtc gcaccagata ccgccgcatt ggccgcactg
    26041 atccagttcg atcagcgagc ccggattggc gcgcacgctg accggcttca tcggcacttc
    26101 gcattgcggg catcgcacca cttcagcgcg ttccttgcgc atcgatggtt tcaactccga
    26161 actgcgctgc aggtcgcggc aaagctccca tctgatacag cagcatgatt cgccgattga
    26221 tcggcggatg gctgcttaat agatcagcga gtttcccgtc gctaccatcg gtgcgccggc
    26281 gcagcgggtt gatgaaaaaa agatgggcgg tgccgcgcgt cgccttttga aacggcatcc
    26341 cggcatcgcg gattttttcc agtgcgtttg ccaaaccctg cggattgcgg gtgaactcga
    26401 cggcggtggc atcggccaaa tactcgcgct gccgtgacac cgccatcgcc aagaggcgcg
    26461 agatcaacgg agagaggatg atcagcagca ccgccggaat cgcaaacaac gcctgagcgt
    26521 ttgggcgacg cacaggccgc gcgccacaga ttgagcgctg agcccaatca gagagcagaa
    26581 ccacaccgcc caaaaggacg gtgatcagca gcatgaccga tgtgtcattg tttttgatat
    26641 gcgccatttc atgggcgacc acaccttgag tttcctcgcg gccgagcaag gtcaacagtc
    26701 ccgtggtaac gcaaatcgat gcggattctt cattgcggcc ggtggcaaat gcgttgggcg
    26761 caggatcgta agtgacatag actttcggca tgcggcagcc tgaggccaac gccatttctt
    26821 tgacaatatt gtgcagttcg cgatgctcgg gtatctggat atcgagtggt tcagcgccga
    26881 ccgaggacag aatcaacttg ctcccctggc gataacctgt gtacgccacc gtcaccgaaa
    26941 tacataaggc gatcagtgta gctatcggaa acgaagcccc gcgcgcgcca aacgcgtcca
    27001 gatagagata atcgatagag agtcctatga gggaaaaaaa gaacaaaaag caaaacacga
    27061 tgaacgcact gatccggcga ttgcgggttt gacgttcagc gaaatctcgc gacttgagaa
    27121 tacccagttt gactgcctaa aagagaaggg acaacttaag actcacgcgc ggcgccgctc
    27181 gttccgtttc gggagcggag aaaaactcgc tcggcttgaa ccggaagaag ctcgcgatca
    27241 tgttcgacgg aaacacttcc tgcttcatgt tatagcgcat caccagatcg ttatagtatt
    27301 ggcgggcaaa ggcgatttga ttttcagtgg tcgtcaattc ttcctgtagc cggagcacgt
    27361 tttggttcgc tttgagatcg ggatagtttt ccatcaacgc gaacattcgc cccagcgact
    27421 gcgttaacgc agtctgcgct tcagcatttt ctttgattcc ggtagaggtc atggctctgc
    27481 ttcgcgcatc gatgactttt tgcaatgtat cctgttcgtg ttgcatgtag cccttgaccg
    27541 tttcgacgag attcggaatc aagtcataac gccgtttaag ctgcacgtcg atctgcttcc
    27601 aggcattctc gatctgatgc cgcagcgcca cgagaccgtt gtaagtccaa atcagccaaa
    27661 ctgcgatcag aaaaataacg gcccagaaaa gccacgtcat cgtccccacc tcccagccaa
    27721 ctcatcgcga accagtatcg attgagtcaa gtcaccaagg gaggcgacac cggtcattgt
    27781 cagaacccgc tgcatctcct cggctagtat tgcgaaaacc cgcgcgaccc cgcgcgcgcc
    27841 gaaagcgccg acaccccaag ctatgggacg tcccaccagc accgcccgcg ccccgagcgc
    27901 aatcgcttta acgatgtcgc cgccgctgcg aattccgcta tcgagcagca ccggcacctt
    27961 agcgccgacc gcggcaacca cttcaggcaa cacttccaag gccgcgcgat tgaaatccag
    28021 aattctgccg ccgtgattag acacgaccag agcatcggcg cccgcattaa ccgcatcatg
    28081 agcatcctcc gcgctcataa tgcccttgac gatcaccggc aatgacactt gctgctttag
    28141 ccattcgatg tctttgggcg aagacttgtg ccctttcctc ggcttaccgt ccttggtgga
    28201 gagaggcact tcatcgccga tcttaaccgg ccgcaccgtg tccatggtga ttcccactgc
    28261 cgcaaagccg agatcctgcg caagcctggc atactccgtg acttcttctc ggccgcggtt
    28321 catataagcc atgaacacga acggcgactt cgccgcgttg cgccaatccc gcgggttgaa
    28381 tttagccgcc tggctgacaa acatcatggc acccacccga tcagtgcctt cggcgacttc
    28441 ccgttccgcg tccttgccaa tcagactgaa actgcccacc ggcgcggtga tcgccgggat
    28501 cggcagcttg tggccaaaga gttcgatcgc aatgtccgga tcggtgatgt cgtggaaaat
    28561 cctttgccgg aacatgtact gacggaatgc acggggattt cgatgaaatg tcgccttggt
    28621 ttcagcggcg ccgttgaaat gatgccaagc ctcagccggc atggttcggc gggcgatctc
    28681 gcggagatcg cggagtccca tttgttccag tggtttttca atcatggatt tagttttgtg
    28741 acgcgatttc ccagcagttg gtgccagggt ctcgaaaaaa gaaggaattt gacgagctgt
    28801 tctcatcgat cgggaacaac tcagtgacac ctagactgtc tccctgttcc gagaacaggc
    28861 gatgtgcctt tttgaccgct tcgttcgatt tgacgctcaa agtattgcga aagttgggcg
    28921 agaaaacttt gaactccttg cgctcggcac aaacgatgta tgttttggta tctggatgct
    28981 tgacgtaaat cacatggtcg ttggcttgat gaacctccaa gctcaacact tcggtgtaaa
    29041 atctacgatt gacgttcatg tcgcgcgcta ccagcgtacc gtgagtaaat ccctgaggaa
    29101 catagcctcg gcctggaaaa cgctcttccg ggaatgggct cgtccagtgc gggccgcgca
    29161 cgccgcctgc ttttgctttg gccgattctt tgcggtttac gacttcataa gattcgattt
    29221 cccagccatt ggttcccggc tcaacgaagt agcaggagta tgatccgtga ttccatagag
    29281 gcttgccgat agcgcccaat ttgtactgag ccttatgcgc gtttagatat tcgtaagccc
    29341 tgtcgacttc gtcgatggac gccacgcgca ccccccaatg attatgcatc tgtttggtcg
    29401 gcgcatccgg accggcctca tgaagaaaca gcagccagtg cgtgtttgga tgcttcagta
    29461 tcgcttcacc cgccttctcg gcaatctttt caaaagccag aagatcgatc agcacagcca
    29521 tggattctcg caaattgcgg cattccatgt gagttgccga aagcgcgatg ggcttgatca
    29581 tgcccttctc tcctgtggat tccatttgat cgctcgttgc cttaccaaac atgtttatga
    29641 aaatttacag cggtatgact accctgtcaa acgagggatt cccgagcgat tccaaatggc
    29701 tcatgctgtt aagcgaagca cccacggttc tgatgaccgt gcggtaagtc atcccgagca
    29761 taccggttgc ttcagtattc aaccatcact cgcaccactg gactgttcat cccgtgaaga
    29821 acaatcagct tgacacaaag ttttcttttc catagattcc gcgcagcttg gcggtgagag
    29881 ggggcagcat gaggttgcta ttccgggggg cagttctgat catgatttgt gttctgccaa
    29941 acgcgccggc agcgacgttc gcgcaagaat ccttttacca ggggaaaacc atccgcatta
    30001 tcgtcggcac ttcggccggc ggcggcttcg acgcctacac gcggaccttg gcgcgccatt
    30061 tcggcaaaca tattccgggt cagcccgcag tcgtagtgga gaacatgccc ggcgccggcc
    30121 acctgattgc cgccaatcat atgtataacg tcgcaaaacc ggatgggctc accatcggcc
    30181 attttcatgg aggctggttt ctctatcagc ttttcaagcg ctcaggactc gaattcgacg
    30241 ctacgaaata cgaatttctc ggatcgccga tcagggaaac ccgcgcttgc gcccttacca
    30301 aggcaagcgg tatcaccagc gtagagcgat ggctggcggc caaaacgccg gtgaagatcg
    30361 gcggtatcgg tggcggcgct cctgatgaca tcgcgcgtat gcttgccgcg accacggcac
    30421 tgccggtcca actcgttgcc ggttacaaag gaacatcgga gattcggctg gcggccgaga
    30481 gcggggaact cgccggcggc tgctggacgt gggattcgat tcgctcgacg tggagtaaag
    30541 ggatccaaag cggtgaagcg gtcgtcgtgc tccagctcgt atctaaacca caccgcgacc
    30601 tgcctaaggt accgctagcc caaaatctcg cgatttcgga agaagcgcgc cagctgctca
    30661 aggccggcat ccaggacacc gcggagttct atcgtccgta cgtggcgccg ccgcgcaccc
    30721 ccaagcagcg gatcgagact ctgcgccaag ctttcgacgc gacgatgaaa gacggcgaat
    30781 ttctcgcgga tgcgcaaaaa gctaacctcg agattgaacc gattaccggg caggagatgg
    30841 aacggctggt atcgggtatc ttcaagctgg atccggcatt ggcggccaag ctcaaaagta
    30901 ttttgaatcc aagctaacgc gccatcgttc ataaacatta cgtcgtatcc ggagggaggg
    30961 tctatgcaga aattactgct cgcgctatcg atggtgcttc tcggcggttt cgccgccgaa
    31021 tcggatcacg cattggcgca ggcaaatttt tttgagggca aagccatccg gttagtcgtc
    31081 ggcttcaccg caggcggcgg ctacgacgcc tacacccgga ccctcgcccg tcatatgggg
    31141 aagcatatcc cgggaaatcc cagcatgatc gtcgaaaata tgcctggcgc cggcagcatg
    31201 atttcagcaa actacacctt taaagccgcg cggccggacg ggcttaccat cggccatttc
    31261 attggtgggc tctttcttca gcagctgcta ggcaaaccgg ggattgaatt cgacgccgct
    31321 aagtttcagt tcgtcggcgt tcccgcccaa gacaacttcg ttatcggatt agccaagtcc
    31381 accggcatca cagacgtcga atcctggatg gcatccaaac aggtggtgaa attcggcggg
    31441 gtcgcctcgg ggtccggctc cgacgatttc ccgaacatcc tcaaagcgac gcttggcctg
    31501 ccgattcaac ttgtctccgg ctacaaaggc acggccgacg ttcgtctcgc gttcaacagc
    31561 ggcgaagttg ccggcctcag caactcctgg gaatcgacca agtcgacgtg gcgcaaggag
    31621 ctggacagcg gcgaattgaa gctgatactt caggcaacgc tcaagccgca tcccgaacat
    31681 gcgaagctgc cgatggcgct caactatgcg aagaccgaag aggcgaagag actgatctca
    31741 acggtcgcgc gcgtgcacgg ccctacggtg cggccgtacg tgctgccgcc caatacgccg
    31801 gcggatcgag tgcagacact gcgccaggca ttcatggcga cgatgaaaga cccggagttt
    31861 ctcgcggaag ccaaaaaggc caacctagac atcaatcccg aagacggcgc gaccctcgag
    31921 caaaacgtca aggagattct gcaactcgag cctgctctcg tcgccaaatt gaaagagttt
    31981 ctgaagtagc tggacatacg agaagaaaaa gcgctcggaa cggttctccg tgctaacagc
    32041 ccgacggtgc gcctgttatt tgtcacgcac cagaccatcg catgcgctcg agcttcttga
    32101 atgcctagag gaggtcttat gaaagtcagc gtggacagaa ccaagtgtga agggtacgca
    32161 agatgcgtgc aggcagcccc taaagttttt cagctggata acaagatgat cgctgaagtt
    32221 gttgatctcc agggcgatag tgatgacaag atccttcttg ccgccaggct gtgtcctacc
    32281 aaagcgattg ccgtggagga ggaaggaacg ggtaagaagc tgtttccggt agaatgacgc
    32341 gtcatgcgga aatagatgct tggagcgctg aagcacggca gcccgggact acggcaactc
    32401 cgatagcaga cggccgtagc cgctgcgcgg aaccctgaca tggagacgtc tctgaatctc
    32461 ccaacattgc gtcacgccgg gatggatcac cggcacgccc aaatcctgct ctagctcttc
    32521 gacaatatcg agaatgcgta gcttgccgcc ttgaaagtag atgccatccg catccggatg
    32581 acgaagaaac aaacgtttgg cttggaaata aatgtccttc gacgaaagcc ggccgacttc
    32641 ttcccaaggt cccgggagct ttccgagttc gagaacttcg aacccggcgt cggtgaaata
    32701 tttcgccacg atgctggtat catcgaagtc atagccgatg ccgacaaact tcttgatctt
    32761 cagcgctttc atcgcggcga cctgatttgt gccggaagta aaaataggaa tgccgaatcg
    32821 tcgttcccat cgctggatga tttccgcttc gcctttgtaa cctagcagca taaacggcgg
    32881 cgtgcccgcc ggatgaatca aatcgacttg gtcttgagcg agttcagcga ttttctcttc
    32941 gtaagcggga atggcgcttt ggaactcttc aattttgccg tggcgaacat tgttaaacag
    33001 aggaatgact ccgataccgt cgggcagcat tttgattaat tcaaccaatg atcccgaacc
    33061 tttggtcggc ttgatcattc caaccatacc gcgccagcaa tcgtaaggca taaatatctc
    33121 tccgagtttg ttagagtttg gtcacctagt ctttctgtaa atccttgcat cgggagcact
    33181 taattccgga ctccgcatag agatgaacac cagatgagag tcgagggcga gcctgaggag
    33241 cgaaggcata gaagcgaata gttccaatgc cgggtatggt gcttggtccg gaatgacaac
    33301 ttcctcaggc gttcgctaaa gcactggagc ttgcagtctc gctatcagcc ggcacctatt
    33361 gtcttcgctc cgcagggctt gccctcgatt tcctgtgtac tccgcgttga tagctcaggc
    33421 ttattcgaag atctcgccac ccatttaaac cgcacaagtc ggctcagatg cacaggtagg
    33481 tgaaccgttc gccgagcgac aacctgcttt cgccgaacta aggctgcgcg agacctatcg
    33541 ggatctcgct acacggagcg cttgcatcgg gcatctagaa aaacttactt caaacgagtc
    33601 ttcggcttcg ccatcgccac tgattctgat cacaagaggc cgaaggtttt cgacttcgcg
    33661 atacgttcga tgacagcggt gatgctgcac tctttcgccg cattcgatct ttactcatcg
    33721 gctcggattt aaatgcaaac ggtttttgct tcatgagtca acttaggctt caaagtgttg
    33781 cgagtctcag ctctaagctt ccagccgatt ccccccaaga gaagcagaca tgccagcagc
    33841 gtactcccgg ccgcctgcac agcccgaaaa gcagccagcg gaaacgtgaa gcaaagaaag
    33901 agaacgctta cgaagaacca gctccagcct gcgatccgct gcaacgatcc ctgcggcgcg
    33961 cgcaatacga tacacagggc cacgaccaac ggatagagaa gccatacaaa aaaataggtg
    34021 aacgcaagag gcgtgaaaat caggatcaac aaaagaagca tggcgtactc aagcgcatcc
    34081 gactctttcg tccgttctgg gtaagacggc atgcagaata agtaaaacac gcacagcgcg
    34141 gttccgacca acacgaccac gatcgtgaca tacttaaaat ctaagtctgc aatattaacg
    34201 aacagttgtt gatcctgctt gcggtgagcg ttgacgggac ggagcagccg gtgggcgacc
    34261 gcaaccagag agtgattggc ccagctaaat ccgcgctgct cacgctgcga gatcgactga
    34321 ttgtcgtagc ggaacattcc cgaagtccag gtattaaggt cctcaagatt gcgctcgaag
    34381 ccacgaaacg gcgaaggaag cgcaaccaca agcaagaaca gaaataccga agccgaaaga
    34441 gtagctttcc agtgtcttcg atatattaaa tagccgatgg cgagcacggg aaaggccttg
    34501 atcgcggtgg cgagcgccac cagagctccg gcactccatt ccctaccgct gcgcaagcag
    34561 gcgaaagcgc caagcatgaa cgcaaggaga agcaggttgg gttgcccgag gatataattt
    34621 tctgtcacgt agggaagaga acagatcccc ggcaggaggt agagtataga ttgcgcgcgc
    34681 gcggcgttgc cggtaaccag ggacaccgac aataacgagg acgcgaccca tgcaaccgag
    34741 tacaagagca ccagaacgat gatcagcggc agctctccga aatagctcgg aatcgcgaaa
    34801 agcaccgcgg ccgtgggagg atacatgaat tggaatgttc cgtcgcctct cggcgcgtaa
    34861 atatcgccac ccttcagcac cgtctctccg atcaaaaacc atttggcata atctttagtg
    34921 ctgccgcgga aataattgtt gattgacaag aacgcgagaa ccgtgatggt gatagcaaag
    34981 aagacgacaa cggctttagg gagttgcacg tcggcaaatc ggtaggcgtt gaacttttcc
    35041 cgggacttca gatcgttggc tttcaacttt gcagagtgag taccgatatc agtaagcggc
    35101 gacttcctga acgacacggt cagaacatgt aggttccgca attgatgtta tagcattgac
    35161 cggtgatata ggctgcggca tcagtggcga gaaacacaac cggtccggca atgtcttccg
    35221 gctccagaac gtgcggcaat ggggtagaat gcaagcgctg catgacttcc tcttcagatc
    35281 gatggccacg cggcatcgcc gtattggcag aacccggaca gatcgcattg acggtgattc
    35341 catccggccc gagctcgcgc gctaaggatt tgacgaaccc gataatcgcc gctttcgagg
    35401 cggcgtagtg cgagaactgt ttcatgccgt agtggccaat gccagaggcc atgctaatga
    35461 tccggccgct tttttgctcc cgcatggccg gcacgaacgc gcgacagcaa agaaaatttc
    35521 cacccagatt gatatccagc gtgcgatccc aatccgcctc gctcatatcg acaaccgatg
    35581 atttaagata aaccccggca tcgttgacca ggatatcgat tcgaccgaaa ttagcgaacg
    35641 tcgcctcggc cagtttcttc acggaaagtt catcggcaac attgacttcg aaccctgacg
    35701 cgtcgccgcc aagtaccttc acctcgtcgg caaccgaaca tgctttttcc gattgcaagt
    35761 cggcgacaac aactcttgcg ccctctcgcg ccattcccat ggcgatagcc cgaccgattc
    35821 cttgagcccc accggttatc accgcgactt tgttttcaag cctcatggca tcaccacgcc
    35881 gccgttgacg tgaagggtct gtccggtgat tgcggcgctc tcgggtgtcg tcagaaaata
    35941 gtacatctcg gcgagatcgc cgggctgtcc ggttcggccc aatggaatcg ccgcatacca
    36001 gcgctcccgt cgagctgcag gagattcttt ttccatcact cgcggcgtat ccgtcggacc
    36061 cggcgccacc gtattaacga gaatcccgtc cggcgccaat tccagcgcga gacttttcga
    36121 aaacgtgata atccctgctt tagcggcggc ataaggcgcg cgcagcgccg atcccgttac
    36181 cccgtagtta gaagcggtat tgaggattct gccgctcttt tgcttctgca tcacgggagc
    36241 tacttcacgg cacatgagga atgtgccttt tagattgctg tcaatcacct gcttccattc
    36301 gttccaggtg aactcctcaa tccgctttgt gtagatgctg ccgccggcga tgttggcgag
    36361 cacgtcgatg cgcctgtggc gtgaaaggac ctcagcaact atggctttga cttcacctgc
    36421 ttgagtaacg tccacagaga aagcatcggc cttgccgcgc gcggcgagga tttcgtgcct
    36481 cacttgctcc gcggccgccc ggtcataatc agcgacggcc acggtgatgc ccgccgctgc
    36541 catccgatga gcgaccgcgc cgccgattcc accacctcca ccgactatta acgctatctg
    36601 atccatgctt tttgctactt caacattgct tgcacgaccc tactgattat ctgacgttgg
    36661 cgtaagcacc ttcgaagttc ccataacctc tttgatggaa ttggtgagat agacaaggcg
    36721 gtggaacaaa cggttcttga gcggccggtc ggctcgtttt tcaccccgat cgttcctgat
    36781 tatctatgct gcttttaaat ccaccgtgca gtgagcacag cgggtggctt tgaccggaat
    36841 ggtcgataag cagtacgggc aatcctttgc ggtgggagcc tcggcctctt ttctcttcat
    36901 gctattgatc attcggatca gcaggaagat gacgaatgca atgatcaaaa aatagataac
    36961 atcattgata aaattaccgt aggcaatcac cgggacgcct ttggctttgg cgtcctgaag
    37021 ggagacgggt atgcccttcg aactatcgag cacatagaac aggctagcga aatcaacttt
    37081 gccgagcagc aatcctagcg gcggcatcag tatgccatct acgagagagg tgacgatttt
    37141 gccaaaagcc gcgccaataa taactgcgac tgccagatca ataacgttgc cgcgcgcgat
    37201 aaattcttta aattctttcc acatttttat tcctcctcaa gcccgccagg ttcacttgga
    37261 taagcaggtt gatgaaattc agaacgggtc acggcatata aagtttatcc gagcagcccc
    37321 gatagaattt cgtaactata tccttcaggt gacgtagcag tgtcaagaag attctgacgc
    37381 aggcacttgt aattcttcgg cgccgcacga gtaaatcgcc tcgatctcgg cacggtatct
    37441 ctccccgacg gcttgccgat ctatcttgat cttttgtaat gccgtaatgc tgcgcagctg
    37501 cttgggaaag tcgttcggaa tgaccgcgat ctttcgaatc tgctcgtaac gatccagttt
    37561 agtattgatc aagtccacgc gctgccgtag cgccccttcg agatcgctat cgctcaatgt
    37621 cgtttccatt cgattcaggg cagccgctat gttgggccgc tcgggtacga tcagagcagc
    37681 gataaaaggt cgatggtcgc ccactaggat cgcctgccga acgaacgagt catcttccag
    37741 cagcgcctcg atagtcccgg gattgatatt cgagccgtca gcacaattaa aaacatcctt
    37801 cttcctgccg ataataaaca gagacccgtc attatcgatt tttcccaaat cacccgtgcg
    37861 aaaccagcca tccgcatcga aagcttcaca cgtcgcttgc ggatcatcga agtacccggg
    37921 cattaccgtg gggccgcgga ccagaagttc gccgtcatct gccaagcgca gctcgacgtt
    37981 gggaaacggt gtgcctagac tccctggttt gacgttgccg acgccgcaca gagtcacgcc
    38041 accgcactca gtggagccat acgaaccgat tagaggcact ccgatcagat cgaagaaccg
    38101 caggatgcgt ggcggcatcg ccgccccgcc gtaggtgata tacttcaccc tcccgcctaa
    38161 agcctgttgg gcggcctgtc gcaggtttgt tctcgtcgct gcaatctcac caacagcatg
    38221 atcgtcggag cccatcagcc ggtcgtctcg atcgagtttt tcgatgcgct cccacaactc
    38281 acgaatgact cccctatcta gaatagcgtt ccacagccgt tccatgactc ttggaacgac
    38341 tgacatcgcg gtcggcctca aaccttgaat aaccgctaaa tctagattct gttccggagc
    38401 ctccatgatt gcagtggttc gaccggtaac cgcgctcttg agaaaaccga aacggccgaa
    38461 gagatgattg agactcagga cgataatcgt cagttcttca gccttgctta agggaatctc
    38521 gccgcggctg gcgaaatttg caagcaggtt attgtgagtt cgcatcacgc ctttcggctc
    38581 accggtggat cccgaagtgt acatgatggt cgctaaatcg tttgcctgaa ctgcttgcag
    38641 cagtcgatcc aattcgctga tgtggctgcg tcggcctcgc tccagcagct cgccaaatga
    38701 tatgcagccg cgcagctctt cggcgctgcc gtctaaaacg acaacaagcg aaagattagg
    38761 caattgcgct ttaatagcga gcagccttgg ggcagaaaca gcatcgacaa agattgcgcg
    38821 gcaccgagaa tgcgccagaa tcttcaacaa ggtaagatcc gaaacgttcg gcgagatcac
    38881 cacgttcggg aaccctccgg ccaacgttgc caggtccgcg cacagccagg gcaaactgtt
    38941 gtcgcccata atcgcgacat tatccccttt agccacccgc aattcgtgca gacccaataa
    39001 ggcgctttga accatcgcgc cgcaatcgcg ccatgactta tcttcgaagc tgccgcaccg
    39061 ttgtatcttt agaaaggtgc ggcagccaac ctcgccaacc cggcgaaaaa acagttgacc
    39121 taagctcgac caagagttct gcgcaacggc tgatgccgaa aataaactca taacggctcg
    39181 aagtgataag gctggtattt ggatctttga ggagcttcga tccgcttcaa tcctctaatt
    39241 tcgtgacggt gggcgtacgc atacaatacg gcttcggcaa catcgtcgat atgcccctgt
    39301 acatacttgc gcgccggcac cgctagccga agcagctcga tgccgcgcgc cgtctgggcg
    39361 ggcgccggcg gtgcgccttt ggtcactcga acgccagaga tatagtagag aaatgcggct
    39421 aaggcttcgg cgcgatgatt ctcgggagcg acatgcggca gaaacgcagc gaggtcgata
    39481 tacacagcgt gaccggcggg cggatgcacc aggggaattc tctccctgag cctgcgccaa
    39541 aggtagttaa cctgctccac ccggctggtt acatatgcat ctgcggcgaa gatctcttcc
    39601 agtgccgtgc cgatcatttg cattgcgctt gccggcggtt gcgcgccgtc gagaaaactc
    39661 tgcagggtcg ccttctgaaa gctaaccgga tcgcggctgc cgatcatacc gcccatggga
    39721 accaacaaat ccttcatggc gctcatggta caaccgtctg ccgagtcgca cgtctccaaa
    39781 acaatttcgc gaatggagcg atcgtgatat ccggcctcgc gttgtcggac aagatagcta
    39841 ttctcgagga tacggcaggc atcgagaaat agcggcacgc cgttggctgc ggccattgcc
    39901 ttaaccttct tgagattgcc gagagagacc ggatggccgc cgcacgcgtt gacgctgagc
    39961 tcgatataga tgcagctgac ctgttgcggg ccgtgctcac ggaaaaccgc gtcaagtttt
    40021 tcgatgtcga gatcgccttt gaaggggtct tctgattgga gattatgggc gccatcgccg
    40081 atgacatcga tcaccctgcc gccgttggac tcgatatgca ttcgcgtcga gggaaacagc
    40141 agattgccgg gaacaacagt acccggctta acatgaagtt ttgcccatat gcgctcagcg
    40201 gcgcgtccct gggtggtcgg aactaagtag ggaaatccga agaacttgcg gtactgcatc
    40261 gacagagaag caaaggctcg actcgcttca gtcgccatgc ccattgccgg ttccaacgcc
    40321 tcgctgccgt ttccagctgc aatttgggcc gtgctcagcg cgctcacacc gctatcggtg
    40381 cagagatcga catagattgc atcttccggc agcagctcac aattagagcc gcaggctttg
    40441 agcgcgcgct cacgctcctg caggctggtc tggcgcaaat gacgaaccga agcgatctcg
    40501 tatgggaccg agaacgcaac gccgaccgct cgttcactca tttataggaa actccgttgt
    40561 tccgccggta aatcgtcgac gattcagcgg gggagttttt gctcgactgg ttcatcttcc
    40621 ggttcctgtt cgagcaccgg cttaatccgc tggtggtcac ttgccgacag gatgttcagc
    40681 gcccgatgaa ccatctttcg ttcggtggca aactttaaat ttttgatcac ctggtccatg
    40741 ggaaaccaac gcgcctcatc aacttcgtga tcatgatcgc gaacgtcgcc cttcaagtag
    40801 cgcagcagaa aaaaatgaac tcgtttataa atgcggatcg gctcgccttc tttggttttg
    40861 tcacggtagg cgtagcggat atcgccgagt ttttttacca ggcgcccttc caacccagtc
    40921 tcttctcgca cttcgcgcaa ggcggtctct tcgatgcgct cgccttcttc aacgtgacct
    40981 ttgggcaagc cccaacgatt gcgcacgtgg atcaatgcaa tctgaattcc atcactgtgc
    41041 ttgcgataca tgacgccgcc ggccgatacc tcgcgaactg ttcgtatgcc atcgctgcgg
    41101 ggccgcgtcc caggggtaag ctcctccttg agccatttaa gatagccgct gctgccttcg
    41161 attaccggca gcgcgatcac ttccggaacc gagtaactgt ggagttctcg aacgcgtttc
    41221 tccaggacgg cgaagcggtc ccggctcgtt ttgataatca ggagctcttc ttcgctgtct
    41281 tctacttttc cctgccatcg ataaaccgac tgcaccgact tgatccgatt gacacaagcg
    41341 gcaagccgct cgtcaaccag cgaacgcgcc agccgatccg cctcttcggg cgatccagcc
    41401 gtcacataaa caacgatgag ctctggcatt actatctacc ttacaaccgc ctttcacacc
    41461 cagtcaatcc gctcgtccgc gccgttgtag cggcacgatt cgcagcccgg cggggtggcc
    41521 attctatgct cttgtcatct gtcgcaggcg gattaattta atatgatgca atcgaaagag
    41581 gagatccgtc agaaagtttg gtccgtcatg cagcgtgaaa aagtggcgcg ctttcccggc
    41641 gcgcaaggaa gaatacccaa ctttgccggc gccgaagctt gcgcgcccct gctcgcggaa
    41701 aaatcctatt ggaagcgcgc caaagcgttg aaagccaatc cggactcacc gcaaagagcg
    41761 attcggcaca aagctcttac cgaaggcaaa atcgtctata tggcggtgcc gcgcctgcgc
    41821 catgagaaac catttgtcga gctcgatcca gggaaacttg attgttcccc ttacgttgcc
    41881 tcctcgattc agggtgcggc caaattcggc agattggtcg gcgttaatca aatgaaaaag
    41941 atcgacctga tcatctgcgg ctcggttgcc gtcaattgcc agggcgctcg agtcggtaaa
    42001 ggcggcggct attcggactt ggaattcgcc ttgttgacgg agaacaaaaa ggtttccgca
    42061 aagacgccga tcgtcacttc ggttcatgcg ttacagatcc tcgacgaaga cctgccgatg
    42121 atcgagcacg atatcccgct cagcgccatc gtcacgccag gcactatcat cgaaattgat
    42181 ccggcttttc cacggcccga aggcatttat tggcgaatgc tgccaccaga gaagatccag
    42241 gctattccga ttctcaaagt tgctgcggcg actcgcgatg cggctaccgc gtgatgtctc
    42301 atttgaagtc cggcggggcc tatgttaact caatgaattc gaggaaaaat cgatgcaaag
    42361 gatagatttc gccaaaggcg atgggctggt ccccgtgatc gtgcaggatt atgaatcgcg
    42421 cgacgtgttg atggttgctt atatgaatga agaagcttgg gagcaaacgc aaaagacggg
    42481 gaaagcgcac tattacagcc gttcccgtaa agggctctgg ttcaaaggcg agcagtcggg
    42541 caacttccaa gtagtaaaaa acgccttcat cgactgcgac aacgatacga ttttgctgca
    42601 ggtcaatcag atcggcggcg ctgcctgcca cgaaggattc cgcagctgct tctttcgcca
    42661 gcgctccggc ggcgagtggg aagtttgcgc cgaacgggtt tttgatccga aagaggttta
    42721 caaaaaatca gtatgagcac ggtgcaactg ttcattacag aggtccgacg ctgatgaagg
    42781 ccggcaaaga attgaagctg ggcattccga aggggagcct cgaggaaatg accatcgcgc
    42841 tcttcagaaa atccggctgg aagatttcga cgacgagccg cagttatttt ccttccatcg
    42901 atgatgatac tctgcgctgc agcctagcgc gtccccagga aatgtcacgt tatgtcgagt
    42961 ccgggactct ggatgtcgcc attaccggca gagattgggt catggagaac ggttccgacg
    43021 ttcagctcgt ctgcgagatg gtttattcga agaccagctt tcgtccgacc cgctgggtgt
    43081 tagccgtgcc gcaggattcg ccgatccaaa agatagaaga gctcggcggt aaacggatct
    43141 ctactgaaat cgtgaactat accaagcgct attttgccga aagagaaatc cctgtcgaag
    43201 tcgagttctc ctggggcgcc accgaggcca aggctgccga aggtttagtc gacgccattg
    43261 ttgaagtgac cgaaaccggc agcacgatcc gcgcccacgg gctcaagatt atccatgagc
    43321 taatggagtc ctgtcctcaa ctgatcgcca atcgggaggc atggcaggac tcgtggaaaa
    43381 gagagaagat cgagcaaatc aaaatgttac tgctgggcgc attgtccgcg gaaaacaaag
    43441 ttggtattaa gctcaacgtg ccggaaacct acctggaaaa agtggtcgag ctgatcccga
    43501 gcatcactgc tccgacagtg gccaacctgc acccatcgcc caatctcaag ggcgtaaagt
    43561 ggtattcggt ggaaagcgtc ctcgaagaat cgaaggtgcg cgatctgatt cctaagctca
    43621 ttcagaacgg cgctgtgggc attatcgaat acccgctgaa taaaattatc tagtcgcaag
    43681 acgtgacgcc agggaaaggc gcgcccgaga tgtagggcga ccagcaggtc gccctacgcg
    43741 cctgtggcct gttgcctctc tcctgtcccg tcccgatgca gaaaatactc cttggcgatc
    43801 tcgggaaaga gcgcatagct cagcacgtcc tcttcgctcc gcgccaggct gccagcttcc
    43861 ttgcgcgcct gatcgagccc cggcttcaac aggtctgccg gtcggcagct gatgggctgc
    43921 ttctttcgca ggacttgatt tttcatctcc tcgctgatct gccccggcgg ctcgccgtaa
    43981 agtcccatca catagttgcg cgtctccatc gcaataactg agtagcgctt gccggtcatc
    44041 acgttcagcg ccgcctggga gcctacgatc tgactggtcg gagtgaccaa cggcggatag
    44101 ccgagatcct tgcgcacgtg cggcatttcg gctagcacgg catcgagctt atcttcggct
    44161 ttctgctggc gcaactgggc aaccaggttg gagagcatgc ctcctgggat ctgcgatacc
    44221 agcacatcgg tcttgatctg attgtttacc gggctctcga attccgcgta atttttgcgt
    44281 acgccgtaga aataatcggc gattttccaa agcttggtca agtccagccc ggtgtcgtag
    44341 ggagtatctc gaagcgccgc gacgagttgt tcggtggcag gctgagaggc accctgggat
    44401 aacggcgcga ttgcagtatc gaggatgtcg gcccccgctt cgatcgccat catgtaattc
    44461 atcggcgaca gacccgccgt acagtgagaa tgcagatgca gcggcagcgg aatgcgagat
    44521 ttgatcttct tgatgatttc gtaagccgca taaggtgcga gcatgccggc catgtctttc
    44581 acgcagataa tctgcacgcc catgtccgcc agcttctcgg caacacgcag gaaatagtcg
    44641 ggagaatgca ccgggctgat agtatagcag accgtcccct cgactaattt gccggtgttg
    44701 agcgctgcct cgatagcggt cttcatgttg cgcacatcgt tcatcgcgtc gaagatgcga
    44761 aacacatcga tgccgttggt cgccgcccgg ccgacgaacg actgcacgat atcatcaggg
    44821 taattcttgt aaccgaccac gttctgcccg cgcagcagca tctgaaagcg cgtcttcggc
    44881 atggcggccc gaagcctgcg caaacgctcc cagggatctt ccttgagaaa cctcagcgtg
    44941 gcgtcgaagg tggcgccgcc ccaggtctcc agggaccaat aaccgatatc atcgagctgc
    45001 gcggcgatcg gcagcatgtc ctccgtgcgc atgcgcgtag caagcagaca ctggtgcgca
    45061 tctcgcagga ccgtttccat aagttctatt ttcgctgcca taggcttcac tgatgaggcg
    45121 cttgagttac tcgtacaact cagtttgctt ttcttcgaga cgttcttttt cagcgtcatc
    45181 gatgacatag ccgccatcga tttttctaag cttggtgatg tcataaaaaa actgggagac
    45241 ttccaaacca tcatcgaact cgtacatttt gatggcgtcc caagggcaga ttttcttgtc
    45301 aagccggtct tccgattccg tgccgatcag ctgaagctga aagcgtttgg gtacagcgtc
    45361 aggcggcacc gggttacggt tgctgtcgta aaaacggctg ccgtattcat agaagatctc
    45421 gccggtctct ttgtgctgct gcaccttggt caaatcgtcg ctataacaca tttgacagcc
    45481 gatgcatttg tcattgtcga ccatgacccg ataaggtttg aggccgccct ggggcagctc
    45541 ttcataaacc agattgatgc actcatcgac cggacaatgc gcctcgcaca aaggtgaacc
    45601 cgcgcaactc gagcagttct catccatcac cgcaagtagt gccgtgcctt tgcgcttttt
    45661 ggttgcttca gccgccatga gcttcgaccc tcgctttctt gaatcgacct ttgaaaaaga
    45721 atcccaatgc aatcgccaac aaaacccacc agtatcgaat cagcccgatg cgcccccact
    45781 ccagccaata gacgaaatta ggagagcgag atatgagctg gcctaagacc tcttgcgccc
    45841 cggcataatc cctcacttcg cgcaacgcct gcgaaagacc ataaagcgcg ccgtcatctt
    45901 gcggattaat tttaagcgcc tgttcgaagt cccggcgcgc ttcgctccat ctatcctgtt
    45961 cggcgaacgt ccagccccgg ttgttcagag ccgcgctaaa atccggcttt cgttccaacg
    46021 cctcattgaa attgtcggct gcggcggcaa gatcgccttt tcgtttaaaa gccactccaa
    46081 gattgttgta cgcttgggga agatcgggtt ttagttcgac gaccgtcttg tactccgcaa
    46141 tcgcagcatc gaaatcgcta cgaatttcgg attcgatcca ggccagcagt gttcgcgctt
    46201 caacatgatc ggggtcaaga agaatcgcct ttcgcaccga cgcttcggcg gcttgcgggt
    46261 cgcggagtct cagcagcgtt atacccaaaa agagatgaag gtcagcttcc tgcggatatc
    46321 gcctgagtaa agcctcaaaa accgctttgg cttctgcggc tttcccctcc gccagataat
    46381 tccgcgcctc ctcccactgc ttttgcttga cgagaggaga agttgtttca gcagctaaag
    46441 atgaacccac ccacgcgagg acggccaacc caacgagcca gcctctcagc atctacttcc
    46501 acatttcatt ggggatttgg tcttctttac cctgctcgat gagcttatcg tttttaaagg
    46561 cctcgcgcag tccgatccaa ctaaaaaaaa ttactaggat cagcattccg acaattggaa
    46621 tgttatccgg cttggttacg atagcgagaa aattctgcaa cccgcttgct tcgtccataa
    46681 aagttaaaca cctgtaaaat aaagataatt taaaagttat gcgatattgt tcccaatgtc
    46741 aacgtcgcca ctgcggcaat cccggttcag tgatgatgat ggtgatggtg gccgcgcctc
    46801 tgctcgttat ggcgaaagat caaggatttg atcacaaccg ggacagcttt tgcatccggc
    46861 aattcttgac cgatatcgat gaagtcgaag tccttgagat cgatgccgtc gatcgaaatg
    46921 atgcccgccc cagggatgag ctcgcgttcg ataatgtttc ccatcaactt ggcaatatcg
    46981 gcgtcaaaga ccaagaccat cggctgtccc tggtccacgt gctccttcat ggcgtcgaca
    47041 agcccttcgg ccagcatgcg aatgagcgga taggacgggc caagctccca gcggatcgcc
    47101 agcgcaacgg cgcgatcgga atccataata tcaaaacgct gcagggcctt ctgaacagcg
    47161 gcggcaatat cggcggatgt gatggcttcg ctttgctcaa actccggcgt aatcacttgc
    47221 aggttgcgca acggcagcaa ttccggatga gaaagataga tggtattgcc gctgacctgg
    47281 acggtgtact gcgacgctcc gataaccgta gcgcgaatgc gtacttcggc cggtcgcaat
    47341 gggattttac cgccggctag ctgattggca cgggcgcgca cccgtcggcc taagtgaaca
    47401 ccaagatccc ccagatttcg cttttcgtaa ccatagacaa actcggacac tccacctgaa
    47461 aacatcaccg catgaatcgg atctttatat tcaaggttgg gggtcaaaag cagatcttgc
    47521 gcctgcgacg agatcggccc gcgttcgagc actgcgaata gcgagtcaca cagcacctgg
    47581 gcaaacttct ccttgtcttc ttcgctcagg ctagcgccga gagcgagctt cacaccggcc
    47641 atcttggcta ctttgagcgc ggtgtcctcc agtcgattga tccggccgtt ctcatccatc
    47701 gcgatcaggc gcgcgccgac ttccatagcg caagtgtcga tgacctttcc cccctgggcg
    47761 attgcgattt tgctggtgcc gccgccgatg tctacattca tgacggtgaa gtcgccgccc
    47821 tcatgatggg tcatgccgac tgcaccggaa ccgtatgcgg ccaggatcga ctcgaggtta
    47881 tgtccagcgg tggcacaaac gaacttaccc gcctctgcgg agaaaagcgc ggcaatcgcc
    47941 tcggcatttt tcttcttcgc cgcttctccg gtaacaatga tcgcgcctgt gtcgatatcg
    48001 ctgggcgtca gattagcttt acggtacgaa tcatggatga agtcattcaa cttattggta
    48061 tcaatggtcg ttttgtcgac gtatggggtt aagaggatcg gcgattcatg gagcatctct
    48121 cgattgacca ccacgaaacg gctcgataac gccatgccct gtcgacgcaa ggtcaggcgg
    48181 gaaaaaatca gatgtgacgt cgacgaaccg atgtcgatgc cgaccgaatt aagcgtaatt
    48241 cgctcggagc gccacatcgg atgatcttca tccatgacga tgtcttcttc atcatcaccg
    48301 tattcatcct cgtgcccctc gtgcaaaatg agtatgtccg tcccaagatc ttccatcgat
    48361 tctagcgatc ctttctgacc cgcatggcgc aaaaatggca agttttcatt gcttaacaca
    48421 agaaacatcg tcttgccact gactgaaaaa cagacttatc agcacgatag acaacccatg
    48481 ggatcggcga taagatgagc cccggcgtca ttcatgggtc aagaggaatt tcaagaactc
    48541 tcctcattct tgaagcgctt tatccggcgc ttcaagtttc ttaaggggat cgaaggcctc
    48601 tgtgtgacgg ccatctgcgc gctgctgcta ctggcggtcg ggccgggtgt cgatcagatt
    48661 aaaaatttat ttccttacgc gccgctggtc tacagcattc tcaccgcggc aatcttgttg
    48721 ggcttgattc tttggaccgt cattcaactc gcacggcgat tttcaccaga gcatgcagcg
    48781 cgctacgtcg agcaaaagca gcccaaactg cgtaataatc taatcaactc tttacaactc
    48841 tatccgcaga tagtcagcgc tcaagctgca ccgggatttt ccgcgccgat ggtgctggcg
    48901 ctgctacgca ccacccgcaa agaagtctcc ggactcaagc tcaacgaatt gctcgacacg
    48961 cgccgggtca ggagcagtct gcgcctgttt gttcttctct tcattccagt gctcgggatg
    49021 gtccttttca acccttcgtg ggttggccag acatttagct tgttgacccg tcccttggat
    49081 cacatgccgc cgtctgaaac agccctagaa gtcaatcctc aaggcgtacg cgtcgcgcgc
    49141 ggcgtgccgg tgaccattga ggcaacagct tcgggagtga ttccgaaatc tctggatctc
    49201 atcacctggg ccggcagcaa tgatcgcggc gagcttgttg gtgaggaaaa aatggcgatg
    49261 gagagtcttg gagaaggtaa gttccgggtc aagttggctc gtttggagag aactgtgagc
    49321 tatcgcgccg tgagcggcgc gttttcttcg ccggtttact ccgccgaagc gatcgatccg
    49381 ccggagatcg gcaacgtgca gctgactttg tatccgccgg cgtacaccgg gctcggcgca
    49441 acctcctcga ccagcggcaa cgtcgaagga ctcaaggggt ctaacctgag gattgctgcg
    49501 actaccacga aggacgtcgt caaagcggaa atcgttcttg ctgatggcaa gcaagttccg
    49561 ctcaagatcg agggacgcaa gctgcaaggt aatctggtcc tctttcagtc acagcagtat
    49621 caaatcctgg tggaggatcg ttatggcttt cggaattcgc cgatatctta tgatctgaag
    49681 gttaaacccg atggttttcc caccatcgac ctgctccgcc ccactgaaga cttggagatc
    49741 aatggcgacg aaatcctgac gcttgaatac agcgcccgtg acgatttcgg tcttgctgaa
    49801 gtgacgctat cggtcaaagt cggagagcgc caagagaaga ttcgactgca gaaagatgac
    49861 gcccgaagga taatcctgcg tgaccaatac cggtgggacc tcggccggct ggcattgcgc
    49921 gagggagagg acgcgttggt caatcttgag gtcaccgaca atgacactgt taccggacca
    49981 aagctgggct cgtcgcgcgc gatcaggctg cggcttaaga atatcaaagg cgagcatcag
    50041 caggttgccg aaatggtccg ggaacttaac aatcgaatgg tggacctgct cggtgatcac
    50101 ttggaaaagc cgatgcccga agaaaacaaa gccgatcgcg gctccgacat gaaccaaaaa
    50161 ttcgagcaga atctggccga agcattgaag caaaccgaag aagtaatgcg cagaaccgaa
    50221 aaggaccggc tttccgacta cgccacctgg tccgatctcg aagcattgaa gcgaaacctg
    50281 cagttcacca aggaagaatt gctcaagaaa caagagcagg ctcagtctgc tgaggaaaac
    50341 ttgagagccc gcgatgagat atcgtccgaa ctggagcgca tgtcgctgct ttcggaagac
    50401 atcagcaaac ggctcaaggc gcaggagatg gcctcgacag cgcaggacct ggcgcgcagt
    50461 caagagcgcc tcatggattc tttggaaaaa cttcagagcg gcgataagaa cctggatacg
    50521 attctcaagc agctttccga gctcaccaag tcactggccg cgctgcagca agccatgtcg
    50581 cagatggcgc agcagctgcc ggatgatttc atgaacatgg aaaatatgcg cagcttaggc
    50641 ttcaacgaaa tgttttcggc gctcgacgaa atccgcaaaa agctcatgca aggcgacatc
    50701 gagggagcgc gccagctcgc tcgggaattg ttcaaccaga tggcgcaaat ggtagcctcc
    50761 ttgcagaatt ctcaacgctc ggcgatggct tcgagcatgg ggcgtatgca aggcgagatg
    50821 tcgcgctcga ccaacgaact gcagcagatc gcgcgcgaac aacaggaaat acttttgaat
    50881 acggaagata tcaataaatc ggcgttaaac gagcatgata atgctttgaa ggacaaactg
    50941 gatcgatttt tggaaaacgc caggaacgaa ttaggaaaat tgaccgagct atttccagat
    51001 cgcgagggac cggacgatcc cgaagccgcc gccaagctcg atgacgcgac gatgaatcaa
    51061 cttcttaaaa acctgatcgc aaagctcctg agcaaggact ttccaggcta tggtgaaggc
    51121 gacgggctgg cccgtaagga gctggcaaaa aagcgcgatc catcacaggc ggagagagcg
    51181 cggcgcgctg aaagtacctt ggacaaactg aagtccgagc taaacagttt gatgggccaa
    51241 ccgatgcagg cgctcaagga atccgatcaa aagcaattgc gcgatctcgc caatcgtcaa
    51301 ggagttttgc gcgaacgcac ggaatctttg cacgagaagc ttgagtcact ttttcaactc
    51361 ttcccgtccc tcgatccaaa aatcgtccag aatatcggcg aagccgggaa atccatgaac
    51421 aatgccaacg accgtctcgg ccagcttgat tcccatggcg cggttccgcc tgaacgtgat
    51481 gcgctggacc ggctttcgca atcgcaacaa cagatgcaat cggcaatgca acaaatggct
    51541 cagcgcggcc aactcggcac catgccgatg actcgcttat tccggcaagg ccgcttcatg
    51601 ccgggaggca tgctgacgcc gctgccgggc atgccgcagt ttccggagtt tgaagttgac
    51661 agcggtttta ccggactgga taccgagaag tttcgtcttc ccggaaagga agaatacaaa
    51721 gctccgcgta attttcggga agaaattctg gaatctctca aacagggcgt tccccctcag
    51781 ctaaaggatc agatcgaacg ctatttcaaa aatctctccg agtaatggcc cctttaaaac
    51841 atcgacccgt attatcagcg atcgttatta tgctcggttt aacggcgcag ctgctcgccg
    51901 ccgaggtcga ggacaaattc aaggacggcc aagagctcct cgacgcctgg cgcattagcc
    51961 aagccgaaga actcgccgcg aagtcagtac gtgacaaccc taagtccgcc gatgcgctgg
    52021 gtttcgatgc ccgggtaaaa ttttaccaag ggcggtatca ggaggctttg gtgtccatcg
    52081 accgggcatt ggccattgat tccaaagaaa aacagcgcca aggattgaag ctgctaaccc
    52141 agttcaccgt cgacgtgatc aaaacgctca agcgttacga gagcgcccat ttcgtgcttt
    52201 acctcgatga aaaacgcgac ggaatattag caacgcatgc ccttgatgct ttggaaaaga
    52261 gctaccaggc ggtaggcgcg gaactaggtt actaccccaa agataaagtc cgcgtcgagg
    52321 tcgcgcccga tgctccgagc ttcaacgcca tctcaactct ctcgttgcgc gacatcgagg
    52381 aaacgggcgc ggtgggcatc tgtaagttca ataagctcat gatcatctcg ccgcgcgcgc
    52441 taagtctcgg ctatcgttgg gtcgattcct taagccatga gtacctccat tatgcgatcg
    52501 tcggcctcac caacaatcag gctccgattt ggctgcacga gggaatggcg cgcttttacg
    52561 aaacccgctg gcgcaaaccg gtgccgccca aagacgcagc cgaggactac ctgacgccgg
    52621 ccaatcaaac cttgctggtg caagctctgg aaaagaacca attcgtcggt ttcaagaaga
    52681 tggaaccctc gctgatccat ttggagacac ccgagcaagt tcaactggct tatgccgagg
    52741 ccgcatcggc gatcgactac atcaaccaaa gcaagggcaa ggccggcgta cgcgagctct
    52801 tggcgacact caatgaaaca tcgacacccg aagcgatttc gaaggtgttg ggcctgtctt
    52861 tcgatgattt cgaatcgcag tggaaaaatt tcctcaagca aaaggggctt aaggaaatcg
    52921 aaggcacccg cgtgcgcagg ctgaaagtta aaaaagatca gaacgaagat caagaaatcg
    52981 tcgagctcaa ggagatccaa tcagcggtcg cgcgtaaccg gactcatctt ggcgaccaac
    53041 tatccaataa gggacgaacc gtagcggcgg ccaacgaata ccagcgcgcc ttggaggcaa
    53101 gcccgcactc tccgattatt cttaacaagc tcggacgggt tttgatccag atgaatcgct
    53161 tccccgaggc gctgaatccc ttgaagaaag cgcttgattt ggatccggac aacggcggca
    53221 cctacgttca actcggccgc atttaccacg cgaccaaaaa atttcaagag agtaaggctg
    53281 cgctcgaaga ggcaattcaa atcaaccctt tcaatccggt tatttttaag ctactgaccg
    53341 aagtctacgc atccttgggc gagaaggaaa aagcgcagca ggcaaaggct tcgctcgaca
    53401 agttactcgg cgcgagctga aagggacctc gatggacgcg gctaaaattc atcaacatga
    53461 aattgccgag atcgaagaat tcgctgctaa acgcgacacg atgctcggcg aaattcgcaa
    53521 ggtcatcatc ggtcaagata gagtcattga agaggtttta atcgcgctgt tcgcccgcgg
    53581 gcactgcctg ttggtcgggg ttccgggtct agccaagacg ctgttgatca gcaccttggc
    53641 ggagatcctg gatctcgatt tcaaccggat tcagtttacg cccgacttaa tgccctcgga
    53701 catcaccggg accgatattc tacaagaaga tccatcgacc ggcaggcggc agttccaatt
    53761 tctcaaaggg ccgatattca cgaacatttt actggcggat gagattaacc gcactccgcc
    53821 caagactcaa gccgcgctat tgcagtccat gcaggaatat aaagtcaccg ccggaggcac
    53881 aacctttccg ctggatctgc cgttttttgt cttggcaact cagaacccca tcgaacagga
    53941 aggcacctat ccgttgcccg aagcgcaact cgaccggttt atgctgaata tcgaaatagg
    54001 ttatcccgaa ttcgaagacg aagtgcagat cgttctgcaa acgacttcca acgaaaaacc
    54061 gtcgccgcgc aaggttatgc aaggcgctgc gatcctgcgc tatcaggaac tcgtcaggcg
    54121 cgttccggtc tcgccgtttg tggtctctta cgccgtggcg ctatcgcaaa gctcacggcc
    54181 gcaagctggc gacgcaccgc agttcgtcag agactatgtg gagtggggcg ctggaccgag
    54241 agcttcgcag tatctgatcc ttggcgccaa ggcgcgcacc attctccaag gtcgctacgc
    54301 cgtgtccata gaagacattc aagcgctcgc gccttcggtt ctacgccatc gagtcgtgcc
    54361 aaactacaag gctcaaggcg aaggattgtc gtccgtagat atcatccagc gtctccttag
    54421 cgaagtgaaa ccgcccaagg acgggaaaac ggcgctgtga agacatggca gaaagtgtcg
    54481 ttcgcctcgt cgagcgacaa ccgttctacc agtgaattga tcaacaagcg ttttaaacga
    54541 tctgcttgta accgttctct gtcggtttgc gggaaaggga taataggaag accaaatcac
    54601 ccggcgcctg cgaacatcct tatgcgctct cgcgagttct cagttaacgt tctgattgca
    54661 caatctggcc gtcctctggt accagctcac cagaagcaac cgattagaat gtctttccag
    54721 cctcgcccgt gtagaagacc ctatccgaag gcgcgatcag cgaaatctcg ctaaaccggg
    54781 ctgctcgcga gccgatcata ctgaacgatt cctccgcgcg ttcaaatgat tggtagcact
    54841 ggctgggtct cgatcttagt tgatgggaag cgttacgacc gaatgagagc agcgcctgat
    54901 tgagttactt tccgctcttc ggatgcgcgc ggcgctcgag cgcggcgcga tcccattctg
    54961 ctagattggc ggctgcttca taggtggtct gcagaaactc gagtaacatc tcgtcaggcg
    55021 attccgactc acggacgcgg tcgtatggca aaacgaattc gctgagatcg tcactgtaaa
    55081 aggccccttg gggattcaca gccgctttag aaaagccggc gggctccgga taggcgtagg
    55141 aataaaatgc agcatacggc acgggtcctg agcccggcca gaaaccgcag ctactaactt
    55201 cgtgcgagta ggcttcttgg gtcacccagt cggggaggtt cggaacgccg cctgggtgtt
    55261 gaggagcttt tcggcccgaa aatcgtgtga ctgccagatc ggcagcgccc caaaagaagt
    55321 gcactggact gcatttgcca acgaattgcg agcgaaaaat ttttaacaca cgatctgctt
    55381 gttggagaat gcgccagaaa cggccggcgt aatcggcatc gtacggtcgc caacaaacat
    55441 cctgatcgaa gggtatcggt tcggcaatct cattgggtct tcgatggatg cgcacgttga
    55501 tatcgagctt gtgcatctct tccataaggt gcccatagaa agaggcaacc gattggggct
    55561 caagcgctag cgcagcagtg cagccatcgc tagagcgcag cctaagctga tgctcgagga
    55621 aattgaagtc gatctgaaag cttctggtcc cgtacggtat cggagaagtc gtcagccccg
    55681 tggctgtcac atacaaagtc gtatgccatg aatggttcac ccaaggactc agtgagaggc
    55741 ggatcttgcc gacgatttgc gtccataagt gtaatgctgc gtaggtctca cgccatgcat
    55801 cgagagacaa actcggccat gcctctgaac ggtcatcaag ggaggcgttc atagcttctc
    55861 aacgtcattt gcgttccact cttcgtgtgc cgggatccat ttgtcggaga tgtcctctct
    55921 ccgggatcat agcaaaactc tactcgtcgg taccagatcc actgctcggc cggcattcgg
    55981 cctctacgca tgcccctagc cggtcttagt ccgatggcgg cgcgaagctc gacccggtct
    56041 aacatgacca ctcagcacca gctacccagt gtgcataccc ccatctacgc agcgccgcgg
    56101 ttatgaagat tgcagaacaa cgtcggttgc catctcactt cctacgtcgc ccgtcgtctc
    56161 gactgcgagc agcttgagtt ctatgtaaac ctatcaaacg cgagcgggga tttgagggct
    56221 acacacaagt ctgctgagat gaaaagcggc aaaaaaccga agtccgatcg agttcaaact
    56281 catcacactc cgctgagctt catccactgc caaactttaa gtggaagctt cctgagcgtt
    56341 cctgcgccgg ggggcgcttg cttttctcgc cgtcttgccg cttttcttct tcgctttctt
    56401 gtctttgccg ctcgtctttt cggccgcttc gtcggcgctt gcaaccagct ctatcaagga
    56461 caccggcgcc gcatcgccat gccgccggcc aattttaatg attcgagtat aacctccggg
    56521 gcgattctgg aaccgggtgg cgattgtctc gaacagctta cggaccaccg tcttatcctg
    56581 aatgatggcg agcgcttggc gcctggcatg gagagtaccc tctttgccaa gcgtaatcac
    56641 acggtccacc cagcgtctga gctctttggc cttgggatcg gtggtttgta tcttctcttc
    56701 acgcaacaga gaagtcacca tattgcgcat cattgcccag cggtggctcg aattgcggct
    56761 gagctttctt cctgaattaa gatgtcgcat aactatgcgg tctctttctc gtcggcacgg
    56821 cgtctgctct cgatctcctc acgaggtgga aaatggtcga ttttcattcc caattcgagg
    56881 cccatctcgc gcaggatctc tttgatctca ttgagcgact tgtgaccaaa attcttggtt
    56941 ttgagcatct cctgctcaga cttctgtacc aattctccga tgtatttgat atcggcgttc
    57001 tgtaaacagt tttgcgagcg cacggaaaat tcgagctcat caacgctgcg aaagaggtta
    57061 tcgttgagcg ggatcgatgg ccggtcatca cgccgttctt gctgcggctc atcttgaaag
    57121 ttaacgaaaa tactcaactg atcctgcagg atctttgcgg catacgccac tgcatcatcg
    57181 ggcttgacgc tgccgtcggt taccacttca aagacgactc ggtcataatc cgtgtgctgc
    57241 ccaaggcggg cattcgtaac agtaaaattt accttctgaa ccggcgaaaa aatcgcgtcg
    57301 atgaacaccg tgtctaccgg cgctccctct tctctgttcc gttccgccgg aacatagccc
    57361 cgccccagct tcgcgatcaa ctccatatcg agcttagcgt ctctggaaag cgtggcgatt
    57421 ttttgttccg ggttgagaat ttcgacattg gcagatccgg ttatgtcctt agccaacaca
    57481 gtagccggac ccttggcttc gactttcaac gtgtgttgct cgccatcatt gagtcgcaac
    57541 ctgacttcct tgagattcaa aatgatgtcg gtgacgtctt ccgtgactcc gggaatcgtc
    57601 gagaactcat gaagaattcc tttcatccgg acggcgacga tcgaagcgcc catcaaggat
    57661 gataacagaa ttcttcgtag cgaattgcca atggtctggc caaaaccgcg ttcgaagggc
    57721 tctgcataaa acttcccata agttgaagtt aaagtctttt cgtccacctc caactgcttt
    57781 ggtttaatca aatctgtcca atgcctatac atagtagaca tagtttctcc cctgcattca
    57841 gaagcttact tcgaatacaa ttcgacgatc agcttctcgt taatcggcat ggtaatatcg
    57901 ctgcgggtgg gaagaatcct gattcttcca ctgcaattgt ctcggtctag ttccgcccaa
    57961 tccggcacgc ctcgtcgctg cacgccttca atggctgtta gaatgcgagc catctgacgg
    58021 ctttgctcct tgaccgagat cacgtcgccc actcgaacct ggtaagatgg gatattcagc
    58081 cgcttgccgt tgaccagtat atgtccatgc cgtaccagca gcctggcttc cgctctagaa
    58141 ctggcaaatc ccaaacgata cgtgacattg tctaagcggc gctctaaaaa caccaacaac
    58201 gtctcacctg taataccttt gattcggcta gcctgatcaa aggtcctgcg gaactgactt
    58261 tccatgagac gatagatgcg cttaaccttc tgcttctcgc gcagctgaat actatactcc
    58321 gaaaatttgg gtctgccttg accgtgttcc cccggcggat agttgcgtcg ttcgatagcg
    58381 catttatcgg tgtaacagcg ctcacctttc agaaataact ttaggttctc gcgcctacac
    58441 aaccggcaaa cagattctgt gtatctagcc aatccttcct cctattttcg ttatcaaacg
    58501 cgtctgcgtt tcggcgggcg gcaaccatta tggggaatcg gagttacgtc cttgatcaag
    58561 ctgatgttga tgccagccga ttgcaatgcc cgtagagcgg attcgcgtcc cgctccggga
    58621 ccccgcacaa aaatttgaac cgtccgcaag ccgtggtcca tggctttttt ggcggcgttg
    58681 tcggccgctt gttgcgccgc aaacggagtg cctttgcggg agcccttaaa gcccatgctg
    58741 ccggcgctcg accacgaaat aacattgcct tgatagtccg tgatcgtgac aatcgtattg
    58801 ttgaatgtcg aatggatgtg aacaacgcct tcggaaatgt tcttgcgccc gcgcttcttg
    58861 cgcgcagcgc ggtcggcgcc cttttggtcc tctggggtct cggtcttttt gccctgtttt
    58921 tcgtccgcgg tatcttcgac tttcgccatt tgattactcc tacaatttac taaccttttg
    58981 acggagcggt tttcttaccg gcgacagtct ttcgcggacc ttttctggtt cgcgcgttgg
    59041 tatgggttct ctggccatgc accggaagat ttctacgatg tcgatggccc cggtaagagc
    59101 ccatttcaat atggcgcctg aggttcgccg acacatcccg tcgcagatcg ccttcaactt
    59161 tgtaattctg atctatcaac tgacgaatct taaccacatc atcatcggca agatcgtcac
    59221 tttttaaatc gagcgagatt ccggcctggt ttaatatctc tcgagataaa ttgcgcccga
    59281 tcccatagat gtaggtaagc gcgacttcca tccgtttgtt tcttgggaga tcgacccccg
    59341 ctaaacgcgc cataatttct ccaatcaaat ccttaaaaac tagccttgcc tctgcttgtg
    59401 tttgctattc tcacatagaa cgcggacaac gcccttgcgc cgaatgactt tacatttgtt
    59461 acaaatcttt ttaaccgaag ctctgacctt cacgatagct cctgttctaa cgctcgcgat
    59521 agatgattct tccacgcgcc aaatcatagg gcgacaattc gattttcacc ttgtcaccag
    59581 gaagaatctt gatgtaatgc attcgcatct taccggatat gtgggccaag accctatgcc
    59641 cgttttccaa ctggacccga aacatggcgt tgggtaaggt ttccaagacg gttcccatca
    59701 cttcgatggc atcttctttt ggcataaact atcgcctagc tgctaatcac ctcagactat
    59761 agctggctca gaatataggg accgtctttg gtgacggcga ccgaatgttc gaaatgggcc
    59821 gctaagctac cgtctttggt gaccgcagtc cagccgtcgg ctttaatttc gacttcaggt
    59881 ccgccaatgt ttaccatagg ttcaatggcc agaaccattc cttctttaaa ccgcaatccg
    59941 cgatcaggtt caccgtaatt tggcaccggc ggctcttcat gcagccgttt tccgatgccg
    60001 tgcccaacga atgctcgaac tactgaatag cctgccccct cggccgtcat ttggactgcg
    60061 gcaccaagat cgcccagcct cttgccttca tataattggt tgatgccctc gtacaatgac
    60121 ttttcggtga tctccatcag gcgcctggct tcctcgctga cttttccgac gccgacagtt
    60181 atcgcagagt cgccataaaa accctcgtaa atgacaccgt agtcgaggcc gacgatgtct
    60241 ccttccttaa gcgtacgatt cgacggtatc ccatgaacta tttcttcgtt tatcgatgcg
    60301 cacaggcaac gaggaaagac ccgtccgccg acggaataac ccttgaaggc cgggatggcg
    60361 ttttttttca aagtcgattc ttccgcgatt ctatccagat cgagtgttgt cactcccggg
    60421 gcgatctttg ccttcagctc ctgtaagact tcggctacaa tcagattcgc tcttctcata
    60481 atctcgattt ctcgagctga tttgagcgaa atcaattggc cggcctcgca agtgcgtcga
    60541 taattcgctt ctgaatatct tccactttcc cgacaccatc aattttccgc aaattaccgc
    60601 gctgcccgta gtaattaacc agaggcgcag tctgggtttc gtatacccgc aggcgagtcg
    60661 tgatggtctc ttcgcgatca tcatctcgtt gctgcaaacg accattacaa cggtcgcagg
    60721 tctcgtcatt gcggggtgga tcaaagttca tatggtaaag agcgccgcag tctcggcaat
    60781 ttcgccgtcc cgctaggcgc tgaactataa tctgctgggg tacttgaatg gacaataccc
    60841 gatccaatgg cagtgccatt gccttcaaga tctcttcaag gctttcagcc tgcggcaggg
    60901 ttctgggaaa gccatcgaga acaaatcctt gggcgcagtc tctttgtttt agccgttcac
    60961 caactaagcg gactataaca tcatcgggaa ctagctcgcc gcgattgaga tagctctcgg
    61021 cttccgcgcc aagcggcgtt ttgtcggcca cagcttttct taggatgtcg cctgtagata
    61081 tttgacaggc ctcgaatcgc tcgcgcaaga gcttcgcctg ggtccctttt cctgcgcccg
    61141 gcgggcctag caaaacgaca cgtacactgg ccaagcctta gccccttctt ccccgcagtc
    61201 gtcctcgctt cataaatccc tcatagttgc gggtaatcaa gtggctctcc atttgcgcca
    61261 cagtatcaag cgccacgccg acaacgatca ataaggcggt cccaccgaag tagaacggga
    61321 cgttaaactg gcttactaag atcgtcggca gcaaggctac aatacacagg tagattgccc
    61381 cgctcagagt gattcgagaa agcacccttt cgatatagtc ggcggtcttc tggccgggtc
    61441 gaattcccgg aatatagcca ccgaatttct tcatattttc cgcgacatcg gttggattga
    61501 acacaaccgc agtatagaaa aatgcaaaga aaatgatcag cagtgcgaaa accaaatcgt
    61561 gcaaccactg tcccgggcta agataggtcg ctgcagcttg aacccaggga tactgggcaa
    61621 attgcgccat agttgctgga aagatcagta ttgaagaagc aaaaatgacc ggaataacgt
    61681 tggccgtgtt caccttcaac ggtagatgag aactctggcc accatacatt tttcggccga
    61741 caacccgctt cgcatactga accggaatac gccgctgtcc ccgctcgaca aagataattg
    61801 cgccgacaac ggctacgatc aaaaccagca tgaagagcgc cactaagacg ttcaactcac
    61861 cttcccggat aaactggaag gtcgtcgtga tcgcagaggg aagacgcgca ataatgccgg
    61921 caaagataat cagcgatatg ccattaccca cgccccgctc ggtgatttgt tcccccaacc
    61981 acatgataaa agctgttccg gacgcgagtg taatcactgt catgatgtga aaactcaaat
    62041 cggcacgcaa cacaatcggc tgaccgccgg tttgcgtccc ggccaacccc aagctgatca
    62101 tagttccttg gatgaccgcg agcacgatgg tcccatagcg ggtatattgc gtgattttgc
    62161 gccgccctgc ttcaccctct tttgacaatc gttcgaggta aggaaaaacc accgtcaaca
    62221 attgaaggat aatagacgcg ctaatgtagg gcataatgcc gagcgaaaat accgaaaatt
    62281 gttcaagcgc gccgccggaa aaaagattga ataatccgaa aatcgtgcct ttatactgtt
    62341 caaagaacgc cgctaaggct tggcggtcga ttcccggcgt cggcacatgg acgccaagac
    62401 gatagactgc gagcatcgcc agcgtaaaca acaaacgttt gcgcaactct ggtatgcgcg
    62461 aggcgttttg aaaaccgcta agcatgagtt gttatcagtt cggcccttcc gccggctgct
    62521 tcgatttttt ccttggcctt ggccgagaag ctatgcgctc tgatcgtcaa acttttcgta
    62581 agcgcaccgt cgccgaggat cttaatccgg cgattcttcc catgcaccag tcctcttgct
    62641 gacagtagct ccggattgat ctcgctgtcc gctgataatg cttccaactg atccagattt
    62701 acgatcgcca cctcgacttt gaagggattg ttaaatcctc gtttcggcag acgcctttgt
    62761 agtggcatct ggccgccttc aaaccctgga cgagttccac cgccggcacg ggcgcgttgg
    62821 cctttgtggc cccgacaagc ggtttttcca tgaccggaac cgtcgcctcg ccccacacgt
    62881 ttttgctttt ttgtcgagcc gggagccggc cttagatcat caagtttcat cttaacggat
    62941 ctccgccaat gtttttccgc gccgttcagc aatctcctcc ggcgctctta aatcgcgcaa
    63001 agcttcgaca gtagctttaa ccatattgtg cggattgttg gaacctaagc acttagtcag
    63061 cacattctga attccggccg cttcaaccac ggcgcgaact ccaccgccgg caatgacgcc
    63121 ggtaccatca gaagcgggtt tgaggataac gtacccggca ccgaatcttc cggtcacctc
    63181 gaaggggata gtgcctgaca tcactggaac cctgatcaga ttcttttttg cttgttccaa
    63241 gcctttgcga attgcttccg gcacttcgtt ggccttgccc attccgcagc cgaccacaga
    63301 gcgattgtcg ccaactacga tcagcgcgct aaagctgaac cgccggccac ctttaaccac
    63361 cttggcgaca cggctaatgt gaacaacctt ttccttgagt tctaatccat gcggatcaat
    63421 gcgttccaaa gacacctcac ctgtgcaggc tgcgctaaaa ttctaaacca ccttcgcgcg
    63481 ctccatcagc cacggctttg acgcgcccgt ggaagagaaa cccattacga tcaaagacaa
    63541 cacgattgat gttcttttct ttacaaactt tcgccaaatt caaaccgact cgcttagcgg
    63601 cttccacacc cttcatgctc ttgatttcat ctggcaagcc ggcggaaagc gtcgaagcgg
    63661 atgctaaagt cactcccttg gcatccgata tcacttgagc gtagatgtgc ctactgcttt
    63721 tatagacaca gacacgcggg cgagcatcgg tgcccaaaac tttttttcgg acgcgctgtt
    63781 gtcgccgtat ccgcccaata ttctttcgct tggttgccat ctctgtaaac ctcgggagct
    63841 atttgccagc cgaacccact gccttaccgg ccttacgctt gataacctct tcgcgatact
    63901 taatcccctt ccctttgtaa ggctcaggct ctcgaagact tctgatcttt gccgcagtct
    63961 cccctatcaa ctgtcgatca gcaccggtta aagtgatgat aacctgccgt tcgatcgtag
    64021 ctgtgacgcc tggtggcaac ggaaaaacca ctggatgaga gtaccccagc gtcatatgaa
    64081 tctcttggcc tttaacctcg gcgcgataac cgacgccatt aatatcgagt atgcgattaa
    64141 atcccttgct gacaccttcg gccatattgg cgatcaattt tcgggtgaga ccgtgcagtg
    64201 ctctgattct ccgcacttct tcaccgcgtt ccacatgaac aaccgtccca tcaactttga
    64261 ccgaaatccc cgacggaatc gccgccgtga gctttccctt cggtccttcc agccgaacgg
    64321 catctccatc tactgcgatt ttaacgcctg aaggcacggt gataggtaat tttccgatgc
    64381 gtgacacgtt tacctcttac aggctaccaa accgaacaga gaacctcgcc gccgacattg
    64441 gcgcgttggg cttctcggtc gactaaaatt cctttagaag tcgagagtac gttgatgccc
    64501 agccccttgc gaatcggggt taactgttgt gcgccaacgt acaccttcaa gcttgggcga
    64561 cttactcgct ttaatcctaa tatgatcggc cgattatctc gatcgaactt caattgaatg
    64621 attagctctt caccggcgcc ttcggccgcc ttaactttct tgtattcctt tacgtaaccc
    64681 tcttcaacga acacctttgc gatattttct ttgacccgcg accaaggcac ggccaccctt
    64741 tgatggcgcg ctctcgaggc gttgcggatt cgtgtcagca tgtctgcgat tggatcggtc
    64801 attcccatat tgctctccgt cctaccagct ggccttaatc aggccgggaa tgtggccctt
    64861 gcgggcgtga tctctgagac aaagtcggca catgcgaaag cggcggtaaa acgcccgaga
    64921 tcgtccgcaa agcgggcaac gattatactc tcggacttta aacttatttg gtctttccgc
    64981 tttgattctt aaacacttct tggccaatgg agtcccccta ctcgtcaatc aaccgaacgg
    65041 cattcctaac tgcttgagta acgcccttcc ctcaggatcg gtctttgccg tcgtggttat
    65101 tgtgacggtc atccctcgga ccttttcaac tttgtcagga ttaatttcgg gaaaaatgat
    65161 ctgttctcta agacccaacg aataatttcc ccgtccgtca aacgagcggt cggaaacgcc
    65221 tttgaagtcc cgcactctgg gtagagccac gtgaatgagg cggtcgagga actcatacat
    65281 ccgatctcgg cgcaacgtca ccatgcaacc gatcggcact ccttcacgca atttgaagtt
    65341 tgctatggcc ttcttcgccc gtgtaatcac cggcttctga cccgcgatag cggtaatctc
    65401 cgcgaccgta gaatccaag
//
LOCUS       DASUVS010000011         4563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1750185, whole genome shotgun sequence.
ACCESSION   DASUVS010000011 DASUVS010000000
VERSION     DASUVS010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750185"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="VF182_00765"
     CDS             <1..1483
                     /locus_tag="VF182_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX6435628.1"
                     /translation="GLGEAGEGRNITHQQKKLNERELREFRTRFDIPLPDEEVVETPF
                     YRPPLDSSETQYLLERRKQLRGFVPERKIRAAPLKTPEAGEYGEFLKGSAEHAMSTTM
                     ATVRILTKLLRHEEIGKRIVPIIPDEARTFGMDSLFRQIGIYSPKGQLYEPVDRETLL
                     YYNEAKDGQILEEGITEAGAMSSFIAAGTSYANLATNMIPFYFYYSMFGFQRIGDLIW
                     AAADSKSKGFLLGATAGRTTLNGEGLQHQDGHSHLLASTVPTLLTYDAAFAYEIAVII
                     RDGLKRMYADGEEYFYYLTLYNESYPMPGMPEGVEDGIVRGLYRFKAAPKGKAHKAHL
                     FGSGPLLREALRAQEILAEQFDVSADVWSATSYKLLRGDALRTKRWNMLHPTAAPKKS
                     YLESLLQNEEGVFIAVSDYLKMVPDQIAPWVPGGLTTLGTDGFGRSDTRSNLRRFFEV
                     DAELTTVATLYALYQKGLLPAQSVEAALKKLEIDPEKSFPFYL"
     gene            1507..2811
                     /locus_tag="VF182_00770"
     CDS             1507..2811
                     /locus_tag="VF182_00770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX6435629.1"
                     /translation="MDVKLPQLAEGVESGTVVNILVSEGQAIKKDQAFMELETQKAVG
                     SIPSPGSGTVTKVHVKEGMEVSVGQVLISIESGVKTAASEATNAAPSTSVNRGQSKAA
                     PAATMDAAAERAPGHKSAESFQYQSKSGLAPPAPPSVRKIAHELGIDLTRVRGSEAGG
                     RIRMADVRAYIKELQDRHQPDMPGQPAPAAPKEVIDFSKWGPVHREKLSPLRRTVSRR
                     MFESWTTIPKINQFDEADVTATLSLREKHGSAYERNGVRLTLTSFILLALARALKKHP
                     MANASLDETAGELILKDYCHIGVAVDTDAGLIVPVLRDVDKKPLLQLSRELQDLTRRT
                     RERKVGLEELQGGTFTISNQGSIGGSHFTPIIYAPQVAILGVGQGQEKPIVIDGKIAV
                     RNMLPLCLAYDHRVLDGANAVRFLKDVIVALQTFDEADVILN"
     gene            2818..4230
                     /gene="lpdA"
                     /locus_tag="VF182_00775"
     CDS             2818..4230
                     /gene="lpdA"
                     /locus_tag="VF182_00775"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX6435630.1"
                     /translation="MEPINTEIVVVGAGPGGYTAAFYAADNGKKVTLVEQSSRLGGIC
                     LNVGCIPSKALLHATEILREAKESGNRGIGFGPAQVNLDQLRSWKDSILDKLGQGIKG
                     LAQKRGVQILHGRGHFEDSQTLRVETSEGQKFIRYEKAIIAVGSKPAMPSAFDLGNKR
                     VMTSTEALEIQDIPHDLLVVGGGYIGMELGTVYAALGSNVVVLEALPSILTGVDPDLV
                     RPVTRIAQKAFKEIRLNTKVLKMATAGKQIKVTTQKDKEQREELYDRVLVSVGRAPNY
                     SDLGLENTQVTKDDKGFIQCNPQQQTDDPKIYAIGDVNGGALLAHRASKEAKIAVEAI
                     LGQASAFDNVIIPAVVYTNPEVAWCGLTENEARDKGIEVKVSKFPWGASGRALTLDRP
                     DGLTKLLIEPESERLLGVGIVGVGAGELISEGVLAVEMGATARDIADSVHPHPTLSET
                     LMEAAEAFYGTATHTISRRD"
     gene            4273..>4563
                     /locus_tag="VF182_00780"
     CDS             4273..>4563
                     /locus_tag="VF182_00780"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX6435631.1"
                     /translation="MTGRPPRLFLIDGSSYIYRAFFALPPAINSAGFPTNATYGFISF
                     MMKLLKRHQPKYLAVALDAGRETFRNQMFVEYKGNRRQAPADLVLQLPDIRRV"
BASE COUNT         1137 a         1201 c         1250 g          975 t
ORIGIN      
        1 cggtttgggc gaggcaggag aaggccgcaa catcactcac cagcaaaaga agctcaacga
       61 acgggagctg cgcgaatttc gcacgcgttt cgatatcccg ctgccggatg aagaagttgt
      121 agaaaccccc ttctatcgtc cgcctctgga tagttctgaa acgcagtacc tacttgaacg
      181 aagaaaacag ttgcgcggct ttgtcccgga acggaaaatc agagccgcac cacttaagac
      241 acccgaagcg ggcgagtacg gagagttcct caagggctca gcagaacacg caatgtcgac
      301 aaccatggcg acggtaagaa tccttacaaa gctcttgcgc catgaggaga ttggcaagcg
      361 tattgtgccg atcatccccg atgaagctag aacgttcggt atggactccc tcttccggca
      421 aatcgggatc tactcaccga aaggccaact gtacgagccg gtcgatcggg agaccctgct
      481 ctattataac gaagccaaag atgggcagat ccttgaagaa ggaatcaccg aagcgggcgc
      541 gatgtcgtcg tttattgccg ccggaacatc ttacgcgaat ctggcgacca acatgatccc
      601 cttttacttc tattattcga tgttcggttt tcaacggatt ggcgacttaa tttgggcggc
      661 ggccgacagc aagagcaagg gctttctatt aggcgccacc gcaggccgca cgacgctaaa
      721 cggtgagggc ctgcaacatc aagacggtca cagccacctc ttagccagca cggtgccaac
      781 acttttgacc tatgatgcgg cgtttgccta tgaaatcgcc gtgatcattc gcgatggtct
      841 taaacgcatg tatgccgatg gcgaggaata cttctattat ctgaccctat ataatgagag
      901 ttacccgatg cccgggatgc ctgaaggggt ggaagacggt attgtcaggg gactctaccg
      961 tttcaaagca gcccctaaag gaaaggcgca caaggcgcat ctattcggca gtggcccatt
     1021 gcttcgtgaa gctttgcgcg cgcaggaaat tctcgctgaa caattcgacg tttcagcaga
     1081 cgtatggagc gccactagtt acaagctact caggggcgat gcgcttcgca ccaagcgctg
     1141 gaatatgctc catccaactg cagcgcccaa gaagtcctat ttggaatccc tactacaaaa
     1201 cgaagagggt gtttttatcg ccgtatcgga ttacctgaaa atggtcccgg atcagatagc
     1261 gccgtgggta ccgggcggcc tgacaactct cggcacggat ggttttggac gcagcgatac
     1321 ccgaagcaat ttgaggcggt tcttcgaagt cgacgctgaa cttaccacgg tggccaccct
     1381 ctacgctctg taccaaaagg gcctcttgcc ggctcagtcc gtcgaggccg cgctcaaaaa
     1441 gcttgagatc gatcctgaaa aatcgtttcc gttttatctt taagccgaat aatttaccaa
     1501 acaaatatgg acgtcaagct tccgcaactc gctgagggtg tcgaatccgg aactgtggtt
     1561 aacattcttg tgtccgaagg ccaggcgatt aagaaggatc aggctttcat ggagctggag
     1621 actcagaagg cggtcggctc gataccatcg cccggtagcg gaacggttac caaagttcac
     1681 gtcaaagaag ggatggaggt ttcggttggc caagtcctga tttccatcga gagtggtgtg
     1741 aagacagcgg ccagtgaagc gacgaatgca gcgccgagca cgtctgttaa ccggggacaa
     1801 tctaaggccg caccggctgc aacgatggat gcagctgccg aacgtgcgcc gggacataag
     1861 tcggccgaaa gctttcagta tcagtcgaag tcgggattag caccaccggc gcctccctcg
     1921 gttcggaaga tcgctcacga acttggcatc gacctgacga gagttcgcgg cagcgaagcc
     1981 ggcggaagaa tccggatggc cgacgtgcgc gcgtacataa aagagctcca agaccgccat
     2041 caacccgaca tgcccggcca gcctgctccg gccgctccga aggaggtcat cgatttttca
     2101 aagtggggtc cggttcaccg cgagaagtta tctccattgc gacgaactgt cagccggcgg
     2161 atgttcgagt cctggacaac cattccgaag atcaatcaat tcgacgaggc agatgtcacc
     2221 gcaacccttt cgcttcgtga gaaacatggc tcggcttatg aaagaaatgg ggtccgcctg
     2281 actttgactt ccttcattct cttggccctg gcacgggcgt tgaaaaaaca tcccatggcg
     2341 aacgcgagtc tcgatgaaac agccggagaa ctaatattaa aggactattg tcatatcggc
     2401 gttgcggtcg acaccgatgc cgggttaatt gtccccgttc tacgagatgt ggataagaaa
     2461 cccttgttgc agctatctcg cgagcttcag gatttaacgc gacggacccg cgagcgaaag
     2521 gttgggctag aagaactgca aggcggcacc tttaccattt ccaatcaagg gagtatcggc
     2581 ggaagccatt tcacaccaat tatctacgcg ccccaagttg caattctcgg tgtgggtcag
     2641 gggcaggaaa agccgatcgt catagacgga aaaatagccg taaggaatat gctgccgttg
     2701 tgcttggcgt atgatcatcg cgttctcgac ggcgccaatg ctgtgcgttt cttgaaagat
     2761 gtcatcgttg cgctgcaaac tttcgatgaa gcggacgtga tactaaattg aattactatg
     2821 gaaccgatca acactgagat cgtggttgtg ggtgccgggc ctggcggtta cacggcagcc
     2881 ttctatgcgg cagataacgg gaaaaaggtc accttggtcg aacaaagctc gcgtctcggc
     2941 gggatctgtc taaacgttgg atgcatcccg tcgaaggcgt tgttacatgc caccgaaatt
     3001 ctccgggaag ccaaagagtc aggcaatcgt ggcatcggat tcggccctgc gcaggttaat
     3061 cttgatcaac tgcgcagttg gaaagattcg attttggata aattagggca aggaataaag
     3121 ggattggcac aaaagcgcgg cgttcaaata ctccacggcc gcggccattt cgaggattca
     3181 cagactctga gggtcgaaac atccgaaggc caaaagttta ttcgttatga gaaagctatt
     3241 atcgctgtag gttcaaagcc ggcgatgcct tcggcgttcg atttgggcaa caagcgcgtc
     3301 atgacatcaa cggaggcgtt ggaaatccaa gatattccac acgacttatt ggtggtcggg
     3361 ggcggttaca tcggcatgga attaggaacc gtttatgccg cgcttggcag caatgttgtc
     3421 gtgcttgagg cattgccatc tattctgacc ggcgtcgacc ccgatctagt tcggccggtg
     3481 actcgaatcg cccaaaaagc gttcaaagaa atccgcctca acaccaaggt cttgaagatg
     3541 gccaccgcag gaaaacaaat caaagtgacg acgcaaaaag ataaggaaca gcgcgaagaa
     3601 ctctatgatc gggtcctggt tagcgtcggt cgcgccccca actacagtga ccttggtctt
     3661 gagaacacac aagtaaccaa agacgacaaa ggcttcattc agtgcaatcc gcaacaacaa
     3721 acagatgatc ccaagatcta cgcgatcggc gatgtcaatg gcggcgcatt actggcgcac
     3781 cgcgcatcca aagaggccaa gattgcggtt gaggcaattc tcgggcaagc gagcgcgttc
     3841 gataacgtca tcattcccgc ggtcgtttac actaatcctg aagttgcctg gtgcggcttg
     3901 acagagaacg aagccagaga caagggcatc gaagtgaaag tatctaaatt tccgtggggc
     3961 gcttccggca gagcgctcac gcttgatcgc ccggacggtc tcaccaagct gctgatcgag
     4021 ccagaaagcg aaaggcttct cggcgtgggc attgtcggag tcggcgccgg tgagttgatc
     4081 agcgaaggag tgctagccgt tgaaatgggc gcaaccgcaa gggatatcgc tgattcggtt
     4141 catccgcacc ccacgctttc ggagaccttg atggaggcgg cggaagcctt ctacggcacc
     4201 gccacgcata ccatttcgcg ccgagattaa ctcagtcgtc cctcgtttcg gataccgcga
     4261 gaagcatcac tcatgaccgg tcggccaccc cggctttttc tcatagacgg cagctcttat
     4321 atttatcgag cttttttcgc tttaccccca gcgattaact ccgcaggttt tcccaccaac
     4381 gcgacgtatg gcttcatcag cttcatgatg aagctcctta agcgccatca gccaaagtac
     4441 ctcgcagtgg ctctcgatgc cggccgagaa acttttcgca accagatgtt cgtcgaatat
     4501 aaaggtaatc ggcggcaagc tccggccgat ctggttctgc aacttccgga tatccgcagg
     4561 gtg
//
LOCUS       DASUVS010000012         5006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1532298, whole genome shotgun sequence.
ACCESSION   DASUVS010000012 DASUVS010000000
VERSION     DASUVS010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532298"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..1312
                     /locus_tag="VF182_00785"
     CDS             299..1312
                     /locus_tag="VF182_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6435632.1"
                     /translation="MKLMKLYVAIIVSLVCCAGFAARSAAQSAFHAKAKEEGRVLIYT
                     SLAASDNKPFQAAFEQAYPGIKLEIYRASGTTILQKILTESRAGAHLADAVLTEGTVL
                     HALKDNKLLVQFDSPERKAFDARFKDKEGFWTDVYPTVHSIPYNTKLVARKDLPRRYT
                     DLLDPKWKGKLAANRNNYMVIAAMLDLYGKEKGEDFIRKLAQQEPQVRSGGTLTATLV
                     GAGEMPLAFMVYANNVENVKESGSPVDWVRVEEPLYAESHPIAIMAKAPHPNAARLLA
                     EFAISKNGQQLISNLGKVPGRSDVQPKIGVDRAKLRMISPEDEAKTAHYQKWFDDLFG
                     KGS"
     gene            2099..3124
                     /locus_tag="VF182_00790"
     CDS             2099..3124
                     /locus_tag="VF182_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6435633.1"
                     /translation="MKLCRTVLRLTLSWALCSLFGLTVPVKSHSQDIVEGAKKEGRFL
                     LYTSLTLQEIDEVAGPFTKKYPFLKVESFRGNALQLLQKITTEARAGRPQADVAQFNS
                     FEAWQLQKLGLLQSYKSPEAEKFPDNFKDPDGYWTTLYMNYLVLGYNPTIVSAADVPK
                     TWDDLLHPRWKGEKFALDRDNAVWYGGLALYWGKEKAAKFMKALASQQPTMRKGHTLI
                     ANLMSSGEFPLGLVYAHRVEEMKSKGMTTIEWVALEPIVATPIVIGIAKNAPSSNAAK
                     LFVDFFLSKEGQVISQKQQFRVPANPDMPPVSAKLDAKNLKISLINRQLADSYQEYDK
                     EYQALFK"
     gene            complement(3176..4669)
                     /locus_tag="VF182_00795"
     CDS             complement(3176..4669)
                     /locus_tag="VF182_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX6435634.1"
                     /translation="MGQATAPFQIVEATIDSIHGAFKSGQLTCRQLVQTYIDRIDAVD
                     KAGPAINSIIAVNSTALAEADRLDDAYRNSGPIGPLHGIPVILKDQIDAQGMATTLGS
                     VLFKDFYPDKDAFIVEKMRHAGAVILGKATLGELGGGDTHGSLFGSTKNPYALERTVG
                     GSSGGSAASVSANLAALGVGQEGLASIRRPSTWNSIVGMRTTAGIVSRAGSYSGWPQK
                     AGTLGPMARTVKDLTLLLDIIAGYDSEDPLTARGFGHIPESFTAFLDKDGLRGARIGI
                     LREPMGYNSEPDSDDFKQVGEVFDRALGELQAGGAELVDPIVIPKLNQLLARRADNPT
                     AGEEAFRIYYGRNSHPPFGSRAEAMASPEFAKVFPGAKNRLKTASDAAKYYEYLIACD
                     ELMTNVLKVMADNRLDAIVHKSVEHQPTLIRDGVNPPHVNHRGAPHLNTFLIFVPTIA
                     VPAGFTRDGLPAGISFLGRPYADGQMIRLAYAYEQATHHRRPPASTN"
BASE COUNT         1201 a         1386 c         1289 g         1130 t
ORIGIN      
        1 gacacgtttc gaaccgagtg cagcatttta ctttctctcg tcgtgagtgt gtgaaacgct
       61 ggaacgattg gacgtttgga acaaccgacg ttgaacgttt gaaacttgaa ctttgaacgg
      121 ctgtttacta ttgcggcgcc ggaacatctg aatcacgata ctggaagaga cgctgagtgt
      181 cggcacacgc cgcttctacc atgccgttgg cgatataggc aacgccgaat tgacacaagc
      241 cggtgctttg gtatcgtcat cagcgcaaga gtcatttttc aagaaggaaa tcgccatcat
      301 gaagttgatg aaactatacg tcgctatcat cgtcagcttg gtatgttgcg ccggcttcgc
      361 cgcgcgcagc gccgcgcaaa gcgcgtttca cgccaaggcg aaagaagagg gacgggtgct
      421 catctatact tccctggccg ccagcgataa caaaccgttc caggccgcgt tcgaacaggc
      481 gtacccgggg atcaagttgg agatctatcg cgcttccggc acgacgattc tacaaaaaat
      541 tctgaccgaa agccgcgccg gagcccacct cgccgatgcg gtcctgacgg aaggcaccgt
      601 cttgcacgcg ttgaaggaca acaaattact cgtgcaattc gactcccccg agcgtaaagc
      661 gttcgatgcc cgcttcaaag acaaagaggg attctggacc gacgtctacc caacggtgca
      721 ttcgattccc tataacacca agctcgtcgc ccggaaggat ctgccaaggc gttacaccga
      781 tctgttggac ccgaaatgga aagggaagct cgcagccaat cgcaacaact acatggtgat
      841 cgccgccatg ctcgatttat acggtaagga gaaaggtgaa gattttatcc gcaagctggc
      901 gcaacaagag ccgcaggttc gctcaggggg aacgctcaca gccaccctgg tcggcgccgg
      961 agaaatgccg ctggcgttca tggtctacgc caacaatgtc gaaaacgtca aggaatcggg
     1021 cagccccgtt gattgggtac gagtcgaaga gccgctttat gccgaatccc atcccatcgc
     1081 catcatggcc aaagcgccac accccaacgc ggcccggctg ctcgcggaat tcgccatttc
     1141 caaaaacggg cagcagctga tcagcaatct cgggaaagtc ccgggacgaa gcgacgtcca
     1201 gcccaagatc ggtgtcgatc gcgcaaaatt gcgcatgatt tcgcccgaag acgaggcgaa
     1261 aaccgcccac tatcaaaaat ggttcgatga tttgtttggc aaaggtagtt gatggacgcg
     1321 cccgcttggg cttttgaacc gaagggaatg ataaagggcc gcgttgtaat ttgatcggac
     1381 caagctgtgt tgttaaatca tcgatacttt cgttcatgaa gagctttgcc cagcttagcg
     1441 ttttaatttt ctcagtgaag tcaaagccgt aagcttcatg cgattcgcgc atgacgtagc
     1501 tcccctcact ccgatcaaat cgcgccgcat attttgattc taagctgtcg cttacctgtc
     1561 aacaaatccc aggctctcaa tgtgatcgcg tcgagtcgtc aacccgttct ccaaccgttt
     1621 gctcgttcca ctcccgatgt tctctttgaa aatcttgcag atttaccaag ccgcccaatt
     1681 cgcacaggtt tcgcaaatca actatcgggt aacgcttggc ctgatctcgg atttcaaaaa
     1741 gccactctcc tcgcactaac caagttgact tacagcgacg cgcacgacgt tccgatcgat
     1801 agagaccaga cgcgatgcaa gtgctcgtcg gctgcaacgg cgggcgtgat aacactcttt
     1861 tcagaaagac ccatttttcg cactaagctg cttgtagtcc aaagccgacc tgaaccccac
     1921 ctatgcttta ttaactcaat tatgtttcgt tagcgccaca tcagcgacgc acgactgctg
     1981 ccagcttcaa caaacagatt gactgattgt ttcgacggac gtataccaag cccattcggc
     2041 gattgcctcg agtcaactgt ctccgttctg cgcgataacg tctgaaaagg aaaaacatat
     2101 gaagctctgc cggacggttc tccgcctcac cctatcctgg gctttgtgct ccctcttcgg
     2161 acttactgta ccagtcaaaa gccactcaca agatatcgtc gagggcgcaa agaaggaggg
     2221 caggtttctt ttgtatacct cgttgactct ccaagagatt gatgaagttg ccggtccatt
     2281 tacgaaaaag tatccgtttt tgaaagtcga gtcgtttcgc ggcaacgcct tacaacttct
     2341 tcagaaaatt accaccgaag cacgcgccgg gcggccccaa gccgatgtcg cccaattcaa
     2401 cagctttgaa gcgtggcagt tgcagaagct cgggctgtta caaagctaca agtcgcctga
     2461 agcagaaaaa tttcccgaca acttcaagga cccggacgga tattggacca ctctctacat
     2521 gaattatctc gtgttgggtt ataacccaac catcgtatcc gcagccgacg tgccaaagac
     2581 gtgggacgat cttttacatc cccgatggaa aggcgagaaa ttcgcgctcg accgcgataa
     2641 tgccgtctgg tacggcggcc tggcgcttta ttggggaaaa gaaaaggcag caaagttcat
     2701 gaaagcgctg gctagccaac aaccgaccat gcgcaaggga catacgttga ttgccaatct
     2761 tatgtcgagc ggcgagtttc cgctcggact agtctacgcg catcgcgttg aggagatgaa
     2821 atccaaagga atgaccacga tcgagtgggt tgcgctcgaa cccatcgtgg cgacaccgat
     2881 tgtaatcggc atagcgaaaa atgcgccgag ttcgaacgcc gccaagttgt tcgttgactt
     2941 ttttctgtcg aaagagggtc aagtaatctc tcagaagcag caattcagag tgcccgcaaa
     3001 cccggatatg cctcccgttt ccgccaaact cgacgcaaag aatctaaaga tttctctgat
     3061 caaccggcag ctagcggata gctaccagga gtacgacaag gaataccaag cgctttttaa
     3121 atagctcgat gcaaatattt gggccgcatg aaatcccttc tgttcgaagc tccggttaat
     3181 ttgtgctggc aggtgggcgg cgatggtggg tcgcctgctc gtaggcgtac gcgagcctaa
     3241 tcatttgacc gtcggcgtaa gggcgtccga gaaagctgat tcccgctgga agcccgtcgc
     3301 gcgtgaatcc cgcgggaacg gcgatcgtcg gcacaaatat caaaaacgtg tttaggtgcg
     3361 gcgctcctcg atgattaacg tgaggcgggt taacaccatc acggatcaag gtcggctggt
     3421 gttctacgga tttatgaaca atggcgtcca gcctgttgtc cgccatcacc tttaagacgt
     3481 tggtcattag ctcatcgcag gcgatgaggt attcatagta ttttgccgcg tcagaagccg
     3541 tcttgagacg attctttgcg ccgggaaaca ctttggcaaa ctcgggcgat gccatcgcct
     3601 cggcacggga cccaaacggc gggtggctgt tccgaccgta atagattctg aacgcttctt
     3661 ctcctgctgt gggattgtcc gcgcgccgag ccaacaattg gttgagcttc ggaatcacga
     3721 taggatcgac aagttcggcg ccgccggctt gtagctcacc aagtgcacga tcgaagacct
     3781 caccgacttg cttgaaatcg tctgagtcag gttcagaatt gtagcccatc ggctcgcgca
     3841 atatgccgat gcgtgcgcct ctcagaccat ccttgtcgag aaaagcagta aagctctctg
     3901 gaatgtgacc aaagccccta gcggttaaag ggtcttcgga atcgtagcca gcgataatgt
     3961 ccagcagtaa cgtcaaatct ttaaccgtgc gggccatggg tccgagagtg ccggctttct
     4021 ggggccaacc ggaatagctt ccggcgcggc tcacaattcc cgcagtggtg cgcattccca
     4081 ctatggagtt ccacgtcgac gggcgacgta tcgaagccaa accttcctgc ccgacaccca
     4141 gagccgcgag gtttgccgaa acgctggcgg cagacccgcc cgaggagccg ccaacggtgc
     4201 gctctaaagc ataaggattt tttgttgatc caaacagcga accatgggta tcaccgccac
     4261 ccagctcacc cagcgtcgcc tttcccaata tgaccgctcc cgcgtgcctc atcttttcta
     4321 cgatgaacgc gtctttgtcc gggtagaagt ctttgaacaa caccgatccc aaggttgtcg
     4381 ccattccctg cgcatcgatc tggtccttca ggatcaccgg gattccatgc agtggaccga
     4441 tcggaccgga attcctatag gcgtcgtcca aacgatccgc ttcggcgagc gccgttgagt
     4501 tgacggcgat gatcgaattg atagccggac cggctttgtc caccgcgtcg atacgatcga
     4561 tatacgtttg gacgagctgg cggcaggtca gctggccgga tttaaaagcg ccgtggatgc
     4621 tatcgatagt tgcttcgaca atctgaaacg gcgctgttgc ttgccccatg ttcgccccct
     4681 tgcagagaat ctaatcttgc cgaaaccata tggcaggcca aaatagatgt ctacctcttt
     4741 gccgccaatt tcactgcggc gaaccgtcag gcatgagttg ttgacttaac gcccagccga
     4801 cgctgcagct tcaattccaa gttgatatct cgtttctcga cgtctgctgt atgcaaccgg
     4861 ctaatcaccg caatcttgcg cccgccgcga cggaacacgc atcacgaaca cgagcgtgtt
     4921 ttagataccc gcggtgagct tgctgtcgtt tttaattttt aggaactagg atgtctcgta
     4981 aacgactgac caagtttggc gataat
//
LOCUS       DASUVS010000013         4144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1096010, whole genome shotgun sequence.
ACCESSION   DASUVS010000013 DASUVS010000000
VERSION     DASUVS010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096010"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VF182_00800"
     CDS             <1..266
                     /locus_tag="VF182_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435635.1"
                     /translation="GFCKKGEGKDFVKNGRITIGGQLPLNTHGGLLSQAHLEGQLHIT
                     EAVKQLRGNEVEPERQVANAQVGIVSGHGGSLAMHATLILGAI"
     gene            263..694
                     /locus_tag="VF182_00805"
     CDS             263..694
                     /locus_tag="VF182_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEX6435636.1"
                     /translation="MSKDERGRASRRGEPYLNEPEAKPFWAACREHKLTAQRCSSCKK
                     IFGFPPQALCPHCLATEHEWVALSGKGTVYSQIAYHRAWHPAYQDKIPYNVSLVDLQE
                     GGRLVTNVIGCAPEEVKIGMPVEVVFEDFPEYTLPKFRPLK"
     gene            complement(699..1781)
                     /locus_tag="VF182_00810"
     CDS             complement(699..1781)
                     /locus_tag="VF182_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435637.1"
                     /translation="MKTGRWTKCMFVGFFMLGPVSMVGAADKAFYEGKTVTVLINYAA
                     GGPTDVEGRIIAKHLAKHIPGKPSVVLQNMGGGGGVSATNYLGEVAKPDGLTVGYFTG
                     ALFRFQLKEGTRVDASKYPFIGAVESVSVSYIRSDVAPGIKRAEDVVKAQRFKAGGLS
                     NGSSKDVRFRLSFDLLGLPYDYVTGYSSSSTARLAVQRNEVQYHDETLPGYRTQVESQ
                     MVQTGMVTPLWYTDLVTPDGEVKTSADVPELLPFTQFYRKVMGKSPSGVKYEALKAAN
                     MSSTNMQRTILLSPGSPEAAAAAMRQAVEALSRDEEFLAEAKKVMRFQPRFQLGDEGI
                     QLYKQVSDISPEIVTFLKDYVAEQKK"
     gene            3258..3743
                     /locus_tag="VF182_00815"
     CDS             3258..3743
                     /locus_tag="VF182_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435638.1"
                     /translation="MADSILVALDETQRIHRFMAHLKATARQGDKIIFLIRTTISTWG
                     DVETHLPMVETGNITAREYRGSAWRFNIEQEKQKAERDLELVCQALRRKGINAEVRMY
                     AGGLSKAVRECAKAGATRLVMMPANYPRIQAVKAFLAALKLFNHHIPAVALLPPRYIE
                     R"
     gene            complement(3873..>4144)
                     /locus_tag="VF182_00820"
     CDS             complement(3873..>4144)
                     /locus_tag="VF182_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX6435639.1"
                     /translation="TGKPRPLRAPFYGLRVVPGITFTMGGILVNGRAEVLNEDEKPIH
                     GLYAAGDAIGGLMGGYRGGYTGGLMQALVTGILAGESASQAALES"
BASE COUNT          925 a         1161 c         1115 g          943 t
ORIGIN      
        1 atggcttttg caaaaagggt gaaggcaagg attttgtcaa aaacggccgc atcaccatcg
       61 gcggccagtt gccgctcaac acccacggtg ggttgttgtc gcaggcccat ctcgaaggtc
      121 agctgcatat caccgaagcg gttaaacaat tgcgcggcaa cgaagtggaa ccggagcgcc
      181 aggtggccaa cgcccaagtc ggtatcgtta gcggccacgg cggcagttta gcaatgcatg
      241 caacgttaat cctgggggca atatgagcaa agacgaacgc gggcgcgctt cgcgccgagg
      301 cgaaccctat ctgaacgagc cagaagctaa acccttttgg gcggcctgtc gggagcataa
      361 actgaccgcg cagcgctgct cctcatgcaa gaaaattttc ggatttccgc cgcaggcact
      421 ctgtccgcat tgcctggcaa cggagcacga gtgggtcgcg ctgagcggca aaggcacggt
      481 ctactctcag atcgcctacc atcgggcgtg gcacccggcc tatcaggaca aaattcccta
      541 caacgtgtcg ttggtcgacc tgcaagaagg aggtcgattg gtcactaacg tcatcggctg
      601 cgccccggag gaggtcaaaa tcggcatgcc ggtggaagtg gtcttcgagg acttcccgga
      661 atacacgctg ccgaaattca ggccgctgaa gtagcggatt atttcttttg ctccgcgacg
      721 taatccttca agaacgtgac gatctccggc gagatgtcgc taacctgctt gtagagttgg
      781 atgccttcgt cgccgagctg gaatctcggc tggaagcgca tcaccttttt ggcttcggcg
      841 agaaactctt cgtccctcga cagcgcctcg acggcttgcc gcatcgcggc ggcggctgct
      901 tccggagaac cgggcgaaag cagaatcgtc cgctgcatgt tggtgctgga catattggcc
      961 gccttcagcg cctcgtactt gacgccggac ggcgacttgc ccatcacttt tcggtaaaac
     1021 tgcgtgaacg gcagaagctc cggaacgtcc gcgctggtct tcacctcgcc gtcgggagtg
     1081 acgagatccg tataccacaa cggtgtaacc atgcccgtct ggaccatctg cgactccacc
     1141 tgagtgcgat agcccggcag cgtttcatcg tgatactgaa cttcgttgcg ctgtaccgcc
     1201 agccgcgccg tcgagctgct gctgtagccg gtcacgtagt cgtacggcag cccgaggaga
     1261 tcgaatgaaa ggcggaaacg cacgtccttg gagctgccgt tgctcaaacc tcctgccttg
     1321 aaacgctgcg ctttcacgac atcctccgcg cgcttgatgc cgggcgccac gtcggatctg
     1381 atgtatgaga ccgaaacgct ctcgacggcg ccgatgaacg gatatttcga tgcgtccacg
     1441 cgcgttcctt ctttcaactg gaatcggaac agcgcgccgg tgaaatagcc gacggtgagg
     1501 ccatccggtt tggcaacctc gccgagataa ttcgtcgcgc tgactccccc gccgccaccc
     1561 atgttctgca gcacgacgga aggcttcccc ggtatatgct tggccaaatg tttagcgata
     1621 atccgtcctt cgacgtcggt cggacctccg gcggcatagt taatgagcac ggttacggtc
     1681 ttgccctcgt aaaaagcctt gtcggccgcg ccgaccatgg atacggggcc gagcatgaaa
     1741 aagccgacga acatacattt tgtccagcga cctgttttca ttgtagttct ccttatcagt
     1801 taggaagtcc gaaaaagccg ctctttaaca tactccggtg tgtgcaatgt tctattggaa
     1861 actgcatgtc tagcaggccg ccgaaaaaga cctatatgct tcgttgcctc tcaatcgcgt
     1921 cgggcaggtt cgtgttcgtg atacctgttc gtgttcgttc ggaattctca atcacgtttg
     1981 cacggacacg agcacgagga tccgcctcac actaggattt tttgagcagc ctgcaagagg
     2041 cttttttcag caccctgctt tctgtccttc cgtgaagagg cgcatcaggc actagaacac
     2101 gcgtggacgt gtcattctta gctggttgtg gacaagttat gatgttcaac tactaagaat
     2161 attaaacagt atattaatgg tctgtagaaa acatagttgc gcgacattta cccttaaacc
     2221 aacacctcgt caaccaattt taacccgcaa tgtttttaat atacctggtc caccgccatt
     2281 cagttctgta accacgctca tgccgttgac gggttgtcat ttatcaaggg ggacgctgaa
     2341 gcattggctt ttgatgcggg agttttggcg cggtaatcaa tattgaatcg tcgcattgtt
     2401 atggttcgat ggaaactttt ctcgcaaatg ccaagcgatt gcttcgtcaa aaggatattt
     2461 tctttacgct gatttttgca acgaaactga tgttgcgctt ttgcacagtc agattgagaa
     2521 gccgggcatg aagattctca aacgggaaga tattacagcc aacgtctcag gtcattggat
     2581 ttggacgatc aacgaaagct gctgcttatt cgagctctgt ttcggaattg gctgttgaag
     2641 ccatttcaag aattcgccgg tgtaaaaggt tcgaagatcc attcccggct tccagagtgg
     2701 caggatgatt tatttgcatt atgttctgca gaaatgattt ccagccagag ctcgttccgt
     2761 gacagagcct ccaatcttta actgaatgca ttagccgggc aactctcctc ccgcttacaa
     2821 agttgtttcg gatcttttag cggaaagtcc gaccgagcca acggcgccgg ccatggcgct
     2881 cggcagtaca cctcggcggc cgtgcgcggc gcgccagatc gagatctagg agtcgatacg
     2941 ccgtcgtcaa tccagccgat gattcactcc ccgtgcttgc ctagagactg gtcctgctgg
     3001 cagccgaatc gcgggacatg cagagtttcg gttgccgtca actgtatacg atcggatcgc
     3061 tgcgtaaact gaaggataca tcgggcaagc tattttcttc gcttcttgct tccaggcgca
     3121 agataacctc acgaatcaga cgtgatatgg ctcccacccg aatcgcggga acggcacgga
     3181 gcttgcgcta ccgctcgctt atccaccctg tgtaaacccg gcacgcactc ctcgaacgac
     3241 ctactcaagg aggcaaaatg gccgactcta ttctagttgc tcttgacgaa acccagcgca
     3301 tccaccgctt catggctcac ctaaaagcga ccgctcggca gggtgacaaa atcatctttc
     3361 taatacgcac tacgatatcg acctggggcg acgtggaaac tcatctgccc atggtcgaaa
     3421 ccggcaacat caccgcgcgg gaataccggg gaagcgcatg gcgattcaac atcgagcaag
     3481 aaaagcaaaa ggccgaacgc gacttggagt tggtgtgcca agcgctccgg cgcaagggga
     3541 ttaatgcaga ggtcaggatg tatgcaggcg ggttgagcaa agcggtgcga gaatgcgcaa
     3601 aagccggcgc tactcgctta gttatgatgc cggcgaatta cccaaggatc caagcggtaa
     3661 aggctttttt agctgctctg aaattattta accatcacat cccggcggta gcgttgctgc
     3721 ctcccaggta tatcgagcga tgatgtctga cagctgcttg gatgaaagct gcgcctgggc
     3781 gaattccgtt ccacacatct cacttaccga atgtggccga gccaatatcg cataacactc
     3841 gtgtgctctc gggtagtgac ttctcggtca atttaactct ccaaggccgc ctgcgatgcg
     3901 ctttctccgg ccaagattcc cgtcaccagc gcttgcatca aaccgccggt gtagccgccg
     3961 cgatagccgc ccatcagccc gccgatggcg tccccggcag cgtataggcc gtgaatcggc
     4021 ttctcgtctt cgttcaaaac ttcggcgcgt ccattcacca ggatgccgcc catggtgaaa
     4081 gtaatgccgg gaacgactcg caaaccatag aatggcgcgc gcagcggtct cggcttgccg
     4141 gtgc
//
LOCUS       DASUVS010000014        14158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_877615, whole genome shotgun sequence.
ACCESSION   DASUVS010000014 DASUVS010000000
VERSION     DASUVS010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14158
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877615"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VF182_00825"
     CDS             complement(<1..664)
                     /locus_tag="VF182_00825"
                     /inference="COORDINATES: protein motif:HMM:NF018699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HEX6435640.1"
                     /translation="MDAKQLFDNGDLRGAISQLTADVKANPLDQRNRIFLFELLCFMG
                     DSQRAERQLDAVAQVSGDATVEIGIGIYRGILRAEASRRQLFQGQSRQPKFLTEPPPY
                     TLFHLKALSDIIERHFNEAELSLKESSDLRKSLHGQIDASYFDDFKDGDDLIAPFLEV
                     IIGADYLWLPLEDLVRLEIRPPRTLRDLLWIPAKVELREQQTIDVFIPVQYYGSSEHA
                     EDP"
     gene            complement(783..2927)
                     /locus_tag="VF182_00830"
     CDS             complement(783..2927)
                     /locus_tag="VF182_00830"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX6435641.1"
                     /translation="MKYCPLCGKNYGDETEFCEVDGAILRISGKKDPYLGTVIRGRYQ
                     VLSKLGEGGMGTVYLAEQVSVGRKVAIKFLLGNYASDDAFISRFRREARLAASLNHRN
                     IVILYDFDQANDGTLFIVMEYLSGQKLSDVIRRDGPLEISRAARLGLQIADGLDAAHR
                     AGVIHRDIKPDNIMVAGADASEEIKLMDFGIARLRDPGNASQITRAGLIVGTPAYMSP
                     EQVEGGEVSDKTDIYALGVVLYEMLSGSVPFRAATPGAVLIKQMQEMPVPLRKLRREL
                     PAAVERAVMQALEKEPQKRQNNMGEVVEQLRNAEEQLRGKGTRAKEPVSGWDRIGQAF
                     RRPDEKRPVRFEPPPEPLPIEPTNARTILETAGPQWAPTQALSLDDLDQPKPPVSTFR
                     RSIVMLALLIVAGVVGTVFYLNGPALISQSFTPKQTTSISPPAPELVSLYVTSDRRNL
                     APKERMTLTAMGKFSDGREQHISSGLKWHSSNSSVITIDSEGRAEAQNEGIAELSAEL
                     KERVSPPVTLVVKAEKPTTLTPTIPTRAPAPSPSLRSLVVVSVKRELQANERMILRAK
                     GQYSDGKEIDINDGVLWQSSDERIASIDSRGRVTAHRSGQVKLNASYEGVASPAISLV
                     VRDPVSFEPEVPAESRQKPQQEKPEKPRAPDIRENLRTATVFWEQGKYSEAMIELEHA
                     LRVAPESKEARSLRIRVMKAWEAEKELNSKKP"
     gene            3637..7026
                     /locus_tag="VF182_00835"
     CDS             3637..7026
                     /locus_tag="VF182_00835"
                     /inference="COORDINATES: protein motif:HMM:NF025689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein IcmF/TssM N-terminal
                     domain-containing protein"
                     /protein_id="HEX6435642.1"
                     /translation="MAFFLKHVEGSKTGQVESFDRDIIRIGRQIDNDLAFDPRRDASV
                     SGYHAEISREGESYFVRDLQSRNGTFVNSRKIDSPIQLEDGDLLQFSGRGPKVVFTTR
                     DPSLPTQFAFKEPPRSAPTEILSAPVSDPQQKPVSKSGLLAKLVAVSIGISCLAAAFA
                     VGFHLGYPWWALLAAGAGVSLLIAAAYLFFRFWRHRRIRRQEAEAAHQEREANLGRGR
                     KNNLEDLKRKWTEVVRALRQSNLQKTGDNALDALPWIIVIGESGSGKSALIKGGGPQS
                     SVLSTASEGPTQNCDWWFFDKLVVLDLAGQYLHQAKQSETATEWQELLDLLKNNRRRE
                     PINGVIVAVSADSLASAPVEKLKEQAAHARERLDEVSQSLGVKFPVYLTVTKCDLIVG
                     FNEFWAGLPDKANAQAAGEVNPDPLHNSDPARFFDKAFHRICERVDRIRLAQIVEEER
                     ETAAQRMFLFPAELKSLQVPLKAFVDVLFRPSSYRDVPFLRGFFLTSGRQSGAPTSRL
                     SRLLGYRYAPAQAASATRDFFLRDLYSTILPSDRNLAMRTAVGRERHRLRWAAGLIAV
                     LAISLVLCIVFTVSFTNNWMELTQLDVTPCTSLSALRGSIGEALRPLDNCRQNISSLD
                     VQPEWKRIASNFGLRQTPRIVPALQQRFLPAFTSAIINPLEKHVDQALARGSGASMVV
                     GAITQRALLMTRCEQSSSCGDLQSANPLSYRVMLSIADPELKGGDVEIERFRRTHEAY
                     LVWQSDSNVFVETRLNDVRRIKDWLARGNLKEESIIESARAQFQPVRATTFWGVGVPL
                     EVESAYTARAWQEGIDPLISGLKKLAADQADVSESVKGFEASYRGRALRQWGEFLAAF
                     SQPERVPIQSQLTRELVNLISAADSPYIKVIDEANANLSVILGDTWQRNELPAWASTL
                     KQFVALKTKVAQAQKSAKSNGKPADAETEAGGYLTIYLEALGQLRGEITTFEKSFKSS
                     QKAFEEGEPSAKATHPILRATWALSMLRNSIGAPEETDRLFWMLLSRPLSLAWRGTLA
                     ESGKYLQQQWEGLLLEIRDYDPGSKGSKIMNFVNQQAGAFLTRQSGRWASRRLLDQSF
                     PFTEGFVQYLSRLRFDAMNPTIFRTEPAFRSEPPFTIVRSS"
     gene            7044..8384
                     /gene="tssK"
                     /locus_tag="VF182_00840"
     CDS             7044..8384
                     /gene="tssK"
                     /locus_tag="VF182_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEX6435643.1"
                     /translation="MSRARSVVWSEGLFLTPHLFQQADRHYESLLNFRLKPLAPYLWG
                     VSELEVDRAGLPNGFFTLYRCSGIMPDGLPVQIPDEDFAPEVRSVKDLFPASAESLDV
                     YLTIPARQADSINFHLENGTGGRARRYQMETVRVSDETTEGNECEIPVAKKNFQITLG
                     GESLEGKVWLKIAQLGRTASGVVSLDESYVAPSIAIAASDSLVGILHRILEVLAAKST
                     ALSQHRRHITEFGSSDIANFWLLHTVNSYVPVLAHFNASPNHHPEQLYLFLVQLVGQL
                     STFALQTDARDVPRYDHENLFETFNELEEKIRFLLETIIPAKYVIIPLEKTPELWYVG
                     QIHDDRLLKSAQFYLAVNAQVPPARLIEGVPSQSKISSPDELGALIGRAVPGVVLAHE
                     PVPPNAIPVKPGFKYFHLHTQGRWWDTISKARRLALYLPDEFPELKLELVAVKE"
     gene            8615..9748
                     /gene="tssA"
                     /locus_tag="VF182_00845"
     CDS             8615..9748
                     /gene="tssA"
                     /locus_tag="VF182_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HEX6435644.1"
                     /translation="MPSSAVLDLEALLVPIPGNKPAGESVRYAGVYDAIQEARRADDD
                     LNKGEWQTETKVADWRAVINLATEALGTKCKDLQIGTWLVEALCKRHGFAGLREGLNL
                     LRELHEQFWDGLYPQIEDGDLEFRAGPLNWMNEKLPASIREIALTGGNTLYAWNQWDE
                     SRQVDNLGRQNPEAMQAAIAEGKITGEQFDKIVQATDRAFFESLFEDLSQCKEQLSRL
                     EKTVDEKFGPDAPSLIKVRNAIDDCHQVVGVILKKKREEDPAYKPESDSAILKAQTTP
                     IPIGREMDRGNSHDNGSSMAISANTSSAGEPRNREEAFQRLAVLAAYLKRLEPQHPVT
                     YLLERAVRWTKMPLDQWLGEVVSNQDVLNQLRETLGIKDRDST"
     gene            9863..10402
                     /gene="tssB"
                     /locus_tag="VF182_00850"
     CDS             9863..10402
                     /gene="tssB"
                     /locus_tag="VF182_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HEX6435645.1"
                     /translation="MARESIQSKLGRVRPPRVHITYDVEVGDAIQLKELPFVVGVLAD
                     LSGRPDEALPPVKDRKFVEIDRDNFDAVLKGMKPRLAYRVDNKLADDDSKLGVELRFN
                     SMDDFEPEQVVNQVEPLRKLAEARKKLSDLMAKMDGNDRLVELMDEVIQNTDALQKLS
                     KEAGPGIAEGAKDSVSKEK"
     gene            10404..11900
                     /gene="tssC"
                     /locus_tag="VF182_00855"
     CDS             10404..11900
                     /gene="tssC"
                     /locus_tag="VF182_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEX6435646.1"
                     /translation="MAERSSQAKAKLSIAEEVQKEGSLLDTIVAESRIARSDTERTRA
                     KDLIGEFVQQVLDGAVVVKKDLETAIAARIAALDEAISAQLNEVMHAPEFQKLEGSWR
                     GLHYLVHQSETGTMMKIRVMNVSKDDLRKDLERAAEFDQSALFKKVYEEEYGTFGGAP
                     FGALIGDYEFGRHPQDLSLLEQISHVAAGAHAPFVAAASPGLLNLDSYTDLTGPRDLA
                     KIFDTVEYAKWKSFRDSEDSRYVGLTLPRTLARLPYGPETVPVESFNFKEEVDGKDHS
                     KYLWSNAAYAFGARLTDSFAKYGWCAAIRGVEGGGLVEGLPTHTFSTDEGEVALKCPT
                     EIAITDRREKELSDLGLIPLVHCKGTDYAAFFGAQSCQKAKKYDTDAANANARLSTQL
                     QYMMAVSRFAHYLKSMMRDKIGSFMSRTDCEKFLNRWIMNYVTEDDNASATVKAQYPL
                     REARVEVAEVPGKPGVYRAIAFLRPHFQLDELTVSLRLVAELPGSARK"
     gene            11968..12456
                     /locus_tag="VF182_00860"
     CDS             11968..12456
                     /locus_tag="VF182_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HEX6435647.1"
                     /translation="MAAVDYFLKLKGVDGESTDHKHKGEIDVESFSWGETNSGTHTGG
                     GGGGAGKVVMQDFHFVMKVSKASPKLMLACATGEHIGEGTLVCRKAGTEQQEFLTVKM
                     TDLLVSSYQTGGSGHSDIVPTDQVSLNYAKIEFEYKEQKPDGSLGGAVKAGYDLKANK
                     KV"
     gene            complement(13141..13380)
                     /locus_tag="VF182_00865"
     CDS             complement(13141..13380)
                     /locus_tag="VF182_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435648.1"
                     /translation="MPEQDGTGTIVDDGIRFKGGEQANHYIDLEPARLRSYFDGSSVF
                     DLTDSMTGAWHRITISTSQRDPDTRRRVQQGEVHL"
     gene            13478..>14158
                     /locus_tag="VF182_00870"
     CDS             13478..>14158
                     /locus_tag="VF182_00870"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6435649.1"
                     /translation="MNSQLTAYDIVPYTGHALAQAHPDRLATIATLFGMKPAEITRAR
                     VLELGCGDGLNLTSVALSLPGAECIGIDRAAAGIRKGQERAQVLGLDNVELRQLDIMD
                     VGESLGRFDFIIAHGIYSWVPEAARDKLLAICKQNLADNGVAYVSYNTYPGGHMRDMA
                     REMMRFHVSEFNDPKQRVTQARSLIKFIIDAVSEPDLYRMVLQKELDRITEFADSSLF
                     HDDLSEYNA"
BASE COUNT         3272 a         3638 c         3936 g         3312 t
ORIGIN      
        1 cgggatcctc agcatgctca ctcgaaccgt aatactgcac gggtatgaaa acatcaattg
       61 tttgctgctc acgcaattca accttggcgg gaatccaaag aagatctctt aaggttcgtg
      121 gtggccgaat ctcgagccgg acaagatctt cgagcggcag ccacagataa tccgctccta
      181 tgataacttc taaaaaagga gctatgagat cgtcgccgtc tttgaaatca tcgaagtagc
      241 tagcatcaat ttgaccgtgc aagctcttcc tgagatcgct gctctcttta agcgataatt
      301 cagcttcatt gaaatgccgc tcgattatgt cggagagcgc tttcagatgg aacagcgtgt
      361 aaggaggcgg ttcagtcaaa aactttggtt gccggctctg cccttgaaat agttgacggc
      421 ggctagcttc ggcgcggaga atacctcgat agatcccaat gccaatctcg accgtagcat
      481 cgccgctaac ctgagccaca gcgtcgagct ggcgttccgc tcgctgggaa tcacccatga
      541 agcacaacaa ttcaaaaagg aaaattcggt tacgctggtc gagtgggttc gcctttacat
      601 ctgcggtgag ttggctgatg gctccgcgca gatcgccatt gtcgaagagc tgcttcgcat
      661 ccatcgtcag tgaccctcca cgctgtctga ccttgaactt cgactcctct gatcgtttta
      721 gggtgtcatc atggattccg ggtttggagc ggttgcaaca agcggtacag agtcgctcaa
      781 gcttagggct ttttagagtt gagttctttt tcagcctccc aggctttcat cactctgatc
      841 cgcagagatc gtgcctcctt actctccgga gctaccctga gcgcatgctc gagctcaatc
      901 atggcttcac tgtactttcc ttgttcccaa aaaaccgtcg cggttctcag attttctctg
      961 atgtcaggcg ctcgcggttt ctccggtttc tcttgttgtg gtttttgccg agactcggcg
     1021 ggaacctcgg gctcaaagct taccggatct cttacgacaa gactgatcgc cgggctggcg
     1081 actccttcgt aactcgcatt cagttttacc tggccactgc gatgagccgt cacccgtcct
     1141 ctagaatcaa tcgacgcgat ccgctcatcg ctgctttgcc agagaacccc atcgttgatg
     1201 tcaatctctt tgccatctga atattggcct tttgccctca gaatcatgcg ctcgttggct
     1261 tgtagctcgc gcttgactga aacaaccacg agcgagcgaa gactcggcga aggcgcaggc
     1321 gcccgcgtcg ggattgtcgg agtcagggtt gttggcttct ccgctttaac aacaagcgtt
     1381 acagggggac tgactcgttc tttaagctcc gcgctgagct ccgctatgcc ttcgttttga
     1441 gcttctgccc ggccttcaga atcgatggtg ataacggaac tattgctgct gtgccatttc
     1501 aatccgcttg agatgtgctg ctcccttccg tcagagaatt ttcccatagc ggtaagcgtc
     1561 atgcgttctt taggagcgag atttcgccta tcggaagtca cgtagagaga gaccaattca
     1621 ggcgcggggg gagaaatgga agtagtttgt tttggcgtga aagactggct tatcagcgcc
     1681 gggccgttca agtaaaagac agttccgact acgccggcta ctatcaaaag ggcgagcatc
     1741 acgatcgatc ggcggaacgt ggagacagga ggcttaggct gatccaaatc atcaagcgat
     1801 agagcctggg taggagccca ctgcgggccc gctgtctcga gtattgtcct ggcgttggtg
     1861 ggctctattg gcaacggttc cggcggcggt tcaaaacgta caggccgctt ttcatcgggc
     1921 ctcctaaacg cttgacctat ccggtcccac cccgatacgg gctctttagc tcttgtaccc
     1981 ttacctctta actgttcttc cgcgttacgc agctgctcga cgacctcacc catattattc
     2041 tgacgctttt gcggttcctt ctccaaagcc tgcatcaccg ccctttctac ggctgctggt
     2101 aattcgcgcc gtagcttacg cagcggaacc ggcatctctt gcatctgctt gattaaaacc
     2161 gccccgggag ttgcagccct aaaaggcaca ctgccactga gcatctcata aagaactacg
     2221 cccaaagcat agatgtcagt cttgtcactg acttctccgc cctctacctg ttccggcgac
     2281 atgtaagccg gcgtcccgac aattaggcct gcgcgggtta tctgactggc gttcccggga
     2341 tcacggagtc tcgcaatccc aaaatccatc agcttgatct cttccgaagc gtctgctcca
     2401 gcaaccataa tattgtccgg cttgatatct ctgtgaatca caccggcacg atgggctgca
     2461 tctaatccgt cggcgatctg caggcccagc cgagcggccc ggcttatctc tagaggcccg
     2521 tcgcgccgaa ttacatcgct cagcttctgg ccgctcaagt actccatgac aataaatagc
     2581 gtgccgtcgt tcgcttgatc aaaatcatag aggatcacga tattgcggtg attcaacgag
     2641 gctgcgagcc gagcttcgcg gcggaatcgg ctgataaacg cgtcgtcgct ggcataattg
     2701 cctagcagga actttatcgc gaccttgcga ccgacgctga cttgctcggc caggtagacg
     2761 gttcccatgc ctccttcgcc gagtttgcta agcacctgat agcggcctct gatgacagtg
     2821 ccgaggtagg gatctttctt tccgctaatt ctaaggatcg ctccgtccac ttcgcagaac
     2881 tctgtttcgt ctccgtaatt cttaccgcac agcggacagt acttcattta attctctacc
     2941 tgtaattctt tacttgcggc gatggatcaa cgcctagtac ctctgtcaac aacgttgaca
     3001 gaagctcttc catagagata actgtgcaaa acctttgccc gctttatagt gagcggctct
     3061 gagccgctca ggacagctat tcgcgcgcgc ccgtgaacgt gacataacca ggtgtgagct
     3121 ttaagttccg aacagatcgg cctacaaaag cattcgacag ggactgactt cttttagttt
     3181 tagcgactta tacaccggaa cgggtggact ctgtcaaggc gacactaacg aattacccgg
     3241 ctgtacattg ccgcgagctc tcgctattga cgtgaaccag gcagctctgc agcagtaaaa
     3301 ttaaggtaag agcaaccttc cgtattaaag acggaaagcg attgccgata cggtccggaa
     3361 cttgaatacc acgtcaagcg cgagtattca ccggattgga gctatgtcga agttagaagt
     3421 ggactcttgt cgtcggctca acgtactatc ggtgacggta atgattgcga tctaagcgac
     3481 aggcggttca tccaagctca acctcatgcg atgccattgg gggaaaacag ttgaatgagt
     3541 gttggcgcgc gaagtcaact tgactgcggt gaactagtct gactagaatc cgcgacacag
     3601 ttacctaaag ctgaatcttc attgtaacgt gagccgatgg cattcttcct aaaacatgtc
     3661 gaaggaagca agactggcca ggttgagtca ttcgatcgag acatcatccg catcggtagg
     3721 caaattgaca acgatctcgc gtttgatccg cgcagggatg catcggtgtc gggataccat
     3781 gcagaaatat ctcgggaagg cgaatcttat ttcgtcagag atctgcaaag tcgaaacgga
     3841 accttcgtca actcgcggaa aatcgatagc cctattcaac tggaggacgg cgatctccta
     3901 cagttctccg gccggggccc caaagtagtc tttaccacgc gcgatccctc gttaccgact
     3961 caattcgcgt tcaaggagcc gcctcgaagc gctccgacag aaattttatc ggcgccggtt
     4021 agcgatcctc agcagaaacc ggtgagtaaa tccggattgc tggcgaagtt ggtcgctgtc
     4081 agtatcggga tctcctgctt ggcggctgct ttcgcagtgg gatttcactt gggttatccc
     4141 tggtgggctt tgcttgcagc tggagccggc gtatcgctcc tgatcgcggc agcgtacctg
     4201 ttttttcggt tttggcggca ccgtcgaatc cgccggcaag aagcggaagc ggcgcaccaa
     4261 gaaagagaag cgaatctagg acgcggccgc aagaacaact tagaagactt gaaacgcaag
     4321 tggactgaag tcgtacgcgc gctgcgacag tcgaatcttc aaaaaacagg ggataacgcg
     4381 ctggatgcgt tgccctggat tatcgtcatc ggcgagtcag gttccggaaa atcagcttta
     4441 ataaaaggcg gggggccgca atcttcggta cttagcacgg cttctgaagg tccaacacag
     4501 aattgcgatt ggtggttttt tgacaagctt gtggttcttg atcttgcggg acagtacctt
     4561 catcaggcca agcaaagcga aacggctacc gaatggcaag agctgttgga cttgctcaaa
     4621 aataatcggc ggcgagagcc gatcaacggc gttatcgttg ccgtatcggc tgattcgctg
     4681 gcttcggccc cggttgagaa gctcaaggaa caggcggctc atgcgcgcga gcgcctggat
     4741 gaggtttcac aatcgcttgg agttaaattc ccagtttatc tcaccgtgac taagtgcgat
     4801 ctgatagttg gcttcaatga gttctgggcc gggctcccgg acaaagccaa tgcgcaggcc
     4861 gccggcgagg tgaatcccga tcccctccac aactccgatc cggcgcgatt tttcgacaag
     4921 gcgttccatc gtatatgcga gagggtcgac cgtatccggc tggcgcagat tgtcgaagaa
     4981 gagcgcgaaa ctgcggctca acgaatgttt ctttttccgg ctgaactgaa gagcttgcaa
     5041 gttccgttaa aagctttcgt ggatgttctg tttcggccga gttcgtaccg cgacgttcct
     5101 ttcttgcgag gattttttct taccagtggc cgccaatcag gagcaccgac gtcgcgcctc
     5161 tcacgcctgc ttggctaccg atatgctcct gcacaagcgg caagcgcaac ccgggacttc
     5221 ttcctgcgcg atctctattc cacgatcctt cccagcgacc gtaatctggc tatgcgcacg
     5281 gcggtgggcc gcgagcgcca ccgtcttcgt tgggctgccg gtttgattgc cgtgctggcg
     5341 atatcgctgg tcctgtgcat cgtttttacc gtgtcgttta caaataattg gatggagtta
     5401 actcagttgg atgtgacacc ttgcacgagt ttgtcggcat tgcggggttc catcggcgag
     5461 gccttgcgac cgctcgataa ttgccggcaa aacatttctt cgcttgatgt gcagccggaa
     5521 tggaaacgga ttgcctccaa tttcggccta aggcaaacgc ctcggatcgt gccagcgctg
     5581 cagcaacgat ttctgccggc attcactagc gcgatcatca atccacttga aaaacatgtc
     5641 gaccaagctc tcgcgagagg ttcgggcgca tccatggtgg ttggcgctat cacgcagcgg
     5701 gctcttctta tgactagatg tgagcagagt agctcatgcg gtgatttgca gagcgcgaat
     5761 ccgctgagct atcgggtgat gttgtctatc gctgaccccg agttgaaggg cggtgacgtt
     5821 gaaatcgagc gttttcgtcg gactcacgaa gcctatttgg tctggcaatc ggattcgaac
     5881 gtatttgtcg aaacccggct gaatgacgtc cgaagaataa aggactggtt ggctcgggga
     5941 aatctaaaag aggagtcgat tatcgagtcc gctagagcgc agtttcaacc ggtgcgtgca
     6001 accacctttt ggggtgtcgg tgtaccgctg gaggtggagt ccgcctacac agcacgggca
     6061 tggcaagagg gaatcgatcc gcttatctca ggcctgaaga aattggctgc ggaccaggcg
     6121 gatgtcagtg agtccgtaaa aggattcgaa gctagttatc gaggccgggc tttgcggcag
     6181 tggggagagt ttttggcggc gttttcacag ccggaaagag ttcctatcca gagccaattg
     6241 actcgcgaac tggtcaattt aatctcggcc gcagactcgc cttacattaa agtgattgat
     6301 gaggctaatg ccaatctgag cgtgattctc ggtgatacct ggcagcgtaa cgagcttccg
     6361 gcatgggcgt cgacgttgaa acagttcgta gccctgaaga caaaagttgc tcaggctcag
     6421 aaatccgcca agagcaacgg caagcctgca gacgcagaaa cggaagcagg aggatacctg
     6481 acaatctacc tggaagcgct cggccagttg cgcggcgaaa tcaccacctt tgagaaaagt
     6541 ttcaagtcgt cgcagaaagc ctttgaagag ggtgagccca gcgccaaagc gactcatcca
     6601 atattgcgcg caacatgggc gctatcaatg ttgcggaatt cgatcggcgc tcccgaagaa
     6661 acggatcgcc tattctggat gttgttgtca cgtccactct cgttggcatg gcgggggacc
     6721 ttggcggagt ccggcaagta tctgcagcaa caatgggagg ggttactcct cgaaatcagg
     6781 gattatgatc caggatccaa gggcagcaaa atcatgaact ttgtcaatca gcaggcgggc
     6841 gcgtttctga ctcggcagag cggcagatgg gcctcgcgaa gactgctgga ccaaagtttt
     6901 ccttttacgg aaggattcgt gcagtactta tcgcgcttac ggttcgacgc catgaatccc
     6961 accatttttc gtaccgaacc agcgtttcgg tccgaaccac cttttacaat tgtccgaagt
     7021 tcttagttgt aggagttgac tagatgagcc gagccagaag tgtcgtttgg agtgaagggc
     7081 tctttttaac gccgcacctg tttcagcagg ccgaccggca ttacgaaagc ttgctcaatt
     7141 ttcggttaaa acctctcgcg ccgtatcttt ggggagtatc cgagctggag gtagaccgtg
     7201 ccgggttgcc caacggcttt tttaccctat ataggtgcag cggcatcatg cccgacggac
     7261 tcccggtgca gattcctgat gaggactttg ctcccgaggt ccgatcggtc aaagatctgt
     7321 ttccggcgtc ggcggaatcc cttgatgttt atctaacgat tccagctcga caggccgact
     7381 cgatcaattt tcacctggaa aacggaacag gcggccgagc gcggcggtat caaatggaaa
     7441 cagtccgggt ttcagatgaa actaccgagg ggaacgagtg tgaaataccg gtagccaaaa
     7501 aaaactttca aattacattg ggcggcgaat cactggaggg caaagtttgg cttaaaatcg
     7561 cgcagctggg acgcacggct tccggggtgg tctcgctgga cgaatcgtat gtcgcgccat
     7621 cgatagccat tgccgcttcg gattcattgg tcggcatcct tcaccgtatt ctcgaagtgc
     7681 tggccgcgaa gagcactgct ctttcgcaac accgtcgaca tatcaccgag ttcggctctt
     7741 ccgatattgc caatttttgg ctgttgcaca cggttaactc ctacgttcca gttctagcgc
     7801 acttcaacgc gtcacctaat caccaccccg agcaactcta cttgttcctg gtgcaactgg
     7861 tggggcagct tagcacgttt gctcttcaga cagacgccag ggatgtgccc cgttatgatc
     7921 atgaaaatct ttttgaaacg ttcaacgagt tagaggaaaa aattcggttt cttctggaaa
     7981 cgatcattcc ggccaaatac gtcattatac cgttagagaa aactccggag ctttggtatg
     8041 tcggccaaat tcatgacgac cggctcttaa agagcgctca gttctatctt gccgtgaatg
     8101 cccaagtccc gcccgcgaga ttgatcgaag gcgtcccgtc acagtcaaag atcagttctc
     8161 cggatgagct cggcgcgttg atcggcagag ccgtcccagg cgtcgtgctc gctcatgaac
     8221 ccgttccccc taatgctatt cccgtaaagc ccggatttaa atattttcat ctgcataccc
     8281 aggggcgctg gtgggatacg atatctaagg cgagaagatt agcgttgtac ttgccggacg
     8341 agtttcccga attgaagctc gaattggtgg cggtgaagga gtagccggcg atgcatttta
     8401 tcgcttgtgc tcgacggggc tgtaaacaag ggcgacgatt ggtgtatctc gtggtataca
     8461 gggcgggcat taaattttcc tgaccatgta tttgaaacga gcgatttagc ggattttaga
     8521 cttcgtcata attggcgtag tgattgctgt tttcgcagtt gagagttgag tcgcttcgga
     8581 gtatgtgacg gaagtctagc aaggagactt tcttatgccc tcgtctgcgg tcttggacct
     8641 cgaagctttg cttgtaccga ttcctgggaa caagccggca ggtgagtcgg tgcgttacgc
     8701 cggcgtttac gacgccatcc aggaggcgcg ccgcgcggat gatgacctga acaagggaga
     8761 atggcagacc gaaacgaaag tggcggattg gcgtgctgta atcaatctcg ctacagaagc
     8821 gctcggaacc aagtgcaagg atctccagat cggcacgtgg ctcgttgaag cgctctgcaa
     8881 aaggcacggc tttgccggtt tgcgagaggg cctcaatttg ctgcgggagc tgcacgagca
     8941 gttctgggat gggctttacc cgcagattga agatggcgac ctcgaatttc gtgccggtcc
     9001 actgaattgg atgaatgaaa aattgccggc gtcgatccgt gaaatcgcct tgaccggtgg
     9061 gaatacgttg tacgcctgga atcaatggga tgagtcaaga caggtcgata acttaggacg
     9121 gcagaatccc gaagccatgc aagcagccat tgccgagggg aaaatcaccg gtgagcaatt
     9181 cgataagatc gtccaggcaa cggaccgggc attttttgag agcctgttcg aggatttgtc
     9241 tcaatgcaaa gaacaattaa gccgcctcga gaagacggtg gacgaaaagt tcggtcctga
     9301 cgctcccagc ttaatcaaag tgagaaacgc catcgatgat tgtcatcagg ttgtcggcgt
     9361 catcctcaaa aaaaagcgag aggaggatcc cgcttataag ccggagtccg acagtgccat
     9421 cttaaaggcg cagaccactc caatcccaat cggtagagaa atggatagag gcaatagcca
     9481 tgataatggt agctcgatgg ccatttcggc aaacacaagc tcggccggcg agccgcgcaa
     9541 tcgcgaagag gcctttcaac gcttagcggt cctagcggca tatttgaaac ggcttgaacc
     9601 gcagcacccg gttacctatc tgctcgagcg cgcggtccga tggactaaaa tgcccttgga
     9661 ccagtggctc ggcgaggttg tgagcaatca agatgtgctc aaccagttgc gtgagacatt
     9721 gggcattaag gaccgagata gcacttgaca ctgcttgcga ccccggagta atttcgcgcc
     9781 agattcccac ggcattgaag tccagcaaat atcaatggca gtctaggcgt cacagttgtg
     9841 gcgcaaatac gaaggagggc taatggccag agaaagtata cagagcaaac tgggtcgggt
     9901 ccggccgccg cgcgtgcaca tcacatacga tgtagaagtg ggagacgcca ttcagttaaa
     9961 ggagcttccg ttcgtcgtcg gagtattagc ggacttatcg ggacgacctg atgaggcgct
    10021 tcctcccgta aaagatcgaa agttcgtcga gatcgatcgt gataacttcg acgcggtttt
    10081 aaaaggcatg aagccgcgcc tggcctaccg tgtggataac aaactcgccg atgatgatag
    10141 caagctcggt gtcgagctgc gtttcaacag catggacgac ttcgagccgg agcaggtggt
    10201 caatcaggtc gaacctctgc gcaagctcgc tgaggctcgg aagaagctct ctgatctcat
    10261 ggccaaaatg gacggcaacg accgtttagt cgaactcatg gatgaagtca tccagaatac
    10321 cgacgcttta cagaagttaa gtaaagaagc cggaccgggc attgctgagg gcgcgaagga
    10381 ctccgttagc aaggagaaat aaaatggcag aaagaagtag tcaggcaaaa gctaaactgt
    10441 ccatcgccga agaagtacaa aaagaaggca gtcttttaga tacaatcgtt gccgaaagtc
    10501 ggatcgcgcg cagtgatacc gagcgcacgc gggccaagga tcttatcggc gagtttgtcc
    10561 agcaggtctt ggatggcgct gtcgttgtca agaaggatct tgaaactgcc attgccgcga
    10621 ggatcgccgc gttggacgag gcaatctccg ctcaactcaa cgaagtcatg catgcgccgg
    10681 aatttcagaa gctcgaaggt tcttggcgcg ggctgcacta tttggttcac cagagtgaga
    10741 ccggcacgat gatgaagatc cgcgtcatga acgtctccaa agacgaccta cgcaaggatc
    10801 tcgaacgagc cgctgagttc gatcagagcg ctctgttcaa gaaggtctac gaagaggaat
    10861 atgggacgtt tggcggcgcc ccattcggcg cgctcatcgg tgactatgag tttggccggc
    10921 atcctcagga cctgtcgctc ctggagcaga tctcgcacgt cgccgccggc gctcatgctc
    10981 cgttcgtcgc ggctgcctcc cccggcctgc ttaaccttga tagctatacg gatctgactg
    11041 ggccgcggga tctagctaag atttttgaca cggtggagta tgcgaagtgg aaatcgtttc
    11101 gtgattcgga agattcgcga tacgtcggac tcacactgcc aaggaccctg gcgcggcttc
    11161 cttacggccc cgaaacggtg ccggtggagt cttttaactt taaagaagaa gtggatggca
    11221 aagaccacag caaatatttg tggagcaatg ccgcgtacgc attcggcgcg cgcttgacgg
    11281 attctttcgc caagtacgga tggtgcgccg cgatccgtgg agtggaggga ggtggtctcg
    11341 tcgagggatt gccgactcat acgtttagca ctgacgaagg agaagtggcg ctgaaatgtc
    11401 ctacggaaat cgcgattacg gatcggcgcg aaaaagaact ttcggatctt ggattaattc
    11461 cgctcgtgca ctgcaagggg acggattacg ccgcattctt cggtgctcag tcgtgtcaga
    11521 aggcaaagaa atacgacacc gacgcggcta atgccaatgc gcgtctctcg acgcaactgc
    11581 agtacatgat ggcagtgtcg cgcttcgctc attacctgaa atcgatgatg cgcgataaga
    11641 ttggcagctt catgtcgcgc acggattgcg aaaaattcct taaccgttgg atcatgaatt
    11701 atgtcaccga agacgacaac gcatccgcaa cagtcaaagc acaataccct ctgagagagg
    11761 cccgtgtgga agtggccgag gtgccgggca agccgggtgt ctatcgcgcg atcgcatttt
    11821 tgaggcctca tttccagttg gatgagctga cggtttcgtt gcggttagtt gccgagttgc
    11881 cgggttcggc gaggaagtaa gacattgaag cctgacgaaa ggcgaatagg tgataacaac
    11941 aactattaac ctgtgacgga ggtcgacatg gcggcagtag actattttct gaaactcaaa
    12001 ggggtcgatg gagagtcaac cgaccacaag cataaaggcg agattgatgt ggagtctttc
    12061 tcttggggcg aaaccaattc gggtacgcac acgggcgggg gtggcggagg tgccggcaag
    12121 gttgttatgc aggacttcca tttcgtcatg aaagttagca aggcgagccc aaagctcatg
    12181 ctggcttgtg ccacgggtga gcacatcggg gaagggacgc tggtttgtcg taaggcggga
    12241 acagagcagc aagaattcct aaccgtaaaa atgacggact tactggtttc ctcttatcag
    12301 accggtggtt cagggcatag tgatatcgtt ccgactgatc aagtatctct caactacgcc
    12361 aagatagaat tcgaatataa agaacagaag ccagacggct cgctcggcgg cgctgtcaaa
    12421 gccggatacg atctaaaagc gaataagaag gtataacgcc ttcaccggat agcgcattat
    12481 ctcatgtcgg ctcgtgtctc gttgctccta tgtagacctg ctatggtgtt ctccgtttaa
    12541 gcagggaagg atatctcggg gcggctgaac aatgtcgggt aggcctttcg agagattacg
    12601 aagttcctgg tcggccaccg ctgagatagt attttccgaa catgatgttt cgccatagtt
    12661 ggccggtcca gtaacgacgg cagcgaaacc gcttcgagct tacaattcga ctccatagat
    12721 cgtcttctct agcacaagcg acatgaagcg aagtcgagac cgcttctgga gcctacgggg
    12781 tgttaacgcc gtgcggggtt gttactgctt cgcaagaatt cggctgcctt cgtgactgtt
    12841 tgtacatact cgatcagttt ctggttaaac gcctttattc cgcgggacgt ctttggcgcg
    12901 acaacgatgt tgcttcaagg cgagtacaaa ggcttgacgg ctttgagcgg gccgcctcac
    12961 tatctcacag atcaacgaga ttttagtaat gatctacgcg cctcgtcgcg catgcactcg
    13021 tttgtaaagg ttgatttgac agcttcagag ccggttttaa gccagcggca tcgatgcgat
    13081 gatctcattg aatgcgatcc ggtcagcggg gaagtgttgc gaaagcgccg gggtagcacg
    13141 tcaaagatga acttctcctt gctggacacg acggcgagta tccggatcgc gctgggatgt
    13201 cgatatggtg attcgatgcc acgcgccagt catggaatcg gtgagatcga atacagagga
    13261 accatcgaaa tagctccgga ggcgggcagg ttcgagatcg atataatgat ttgcttgttc
    13321 cccgcctttg aagcgtatgc cgtcatcaac gatggtccca gtgccatctt gttccggcac
    13381 gcaccaccag ctggagtcct cggtctcgga cctccgcgtg gggcgaagcg ccgcatccgg
    13441 tcacgattgg acggtgaact tttcaagcga tcaaacgatg aacagtcaat taacggcata
    13501 tgacatcgtg ccgtatacag ggcatgcgct tgcgcaagcg caccctgatc gactggcaac
    13561 gatcgcgacg ctctttggaa tgaagcctgc ggagattacc cgtgctcgcg tgctcgagct
    13621 cggttgtggt gatggtctca acctgacttc ggtcgcattg agcttacccg gagcggaatg
    13681 tatcggcatc gaccgcgctg cggcaggaat ccgcaaagga caggagcgcg cgcaggttct
    13741 cggtcttgat aatgtcgagc ttcgccaact cgacatcatg gatgtcggag agagtctggg
    13801 ccgcttcgat tttattattg cccatggcat ttattcctgg gtcccggaag cagcgcgcga
    13861 caagctcctg gcgatttgca agcagaacct ggccgacaat ggagtggctt acgtgagcta
    13921 caacacctat ccgggcggcc acatgcggga tatggcccgc gaaatgatgc gctttcacgt
    13981 aagcgagttt aacgatccca agcagcgcgt cactcaggct cgctcgctga ttaaattcat
    14041 tatagatgcc gtcagcgagc cggatctata ccgcatggtt ctgcaaaaag agctggatcg
    14101 gattacagag tttgccgaca gctcgctttt tcatgacgac ttatccgagt acaacgcg
//
LOCUS       DASUVS010000015        10923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_441565, whole genome shotgun sequence.
ACCESSION   DASUVS010000015 DASUVS010000000
VERSION     DASUVS010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10923
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441565"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..114
                     /locus_tag="VF182_00875"
     CDS             <1..114
                     /locus_tag="VF182_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX6435650.1"
                     /translation="GFDPASASAPFVATLVDVTGLIIYFTVATLILRGSLL"
     gene            complement(166..564)
                     /locus_tag="VF182_00880"
     CDS             complement(166..564)
                     /locus_tag="VF182_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6435651.1"
                     /translation="MIKTMGVYHIGIPCNDIDRAVKFYTEILGMTIAKLNRDDMGKGL
                     SRADLRCGDSMVVLFQRPKALDGEFIEREGATHHAFHVTREDFELAKQKMKDWGVRIH
                     STPAVDRPSGSGFYFFDSEGNLLQLYAPPK"
     gene            complement(757..2991)
                     /locus_tag="VF182_00885"
     CDS             complement(757..2991)
                     /locus_tag="VF182_00885"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6435652.1"
                     /translation="MTQVVGRPTPRIEGELKVTGRALYSADVRLPDILWGKVLRSPIS
                     SGRIKKIHTDKARQMSGVKAVITGEDVAGLRIGRCIYDTPILADGLVRFIGEKVAAVA
                     AETRHAAEQALELIEVEYDEMEPLVDPLAAIKDDAPILHPDLLSYKGLPVPVEQVSNV
                     FAYLKWGKGDIETGFRQADVIVENTFTTQVTHQSYLEPHACVVSADSDGGANIWSCSK
                     TPFAVRNQLSNCIGIDKEKLVFHPMHIGGDFGGKGGFMDVPVAYFLSLKSGQPVKMVM
                     EYGEELTAGNPRHAAIIRAKTGVKKDGAIVAHHLDFIFDSGAYGSMKPAGYLIGASTC
                     AGPYKIANCLIEERMVYTNKIPCGHMRAPGDPQGFFANESQMDEVARKLGMDPAEFKR
                     KNLMHDGDETPIGGHVSHIRGVEALEKAIEMSGYSKPRPKNIGRGLAFSEWSPSGGEG
                     NVFVKIEEDGKVKVSSPVVDQGAGVLTVIVECVGQELGVPAGEIQLAQLDSTTVPSDG
                     GVGGSRATRVYGNASYEAGVKARQEIFAAAAAKLKVPAEELELADSQVIHRKSKRTMS
                     FADVIKTKGSPISVKGYYKSSEKSHDASVSAQIAEVQVDPETGQVILKQMVSAHTTGK
                     VINPLMHQGQIDGGIVFGLGYALQEEILFDNGKIVTTNFGEFKIPNIMDIPPLKTSVI
                     ENVPHGPGPYNSLAIGEVANTPTAAAVANAVADACGVRITDLPLTAEKVYQALRGSHK
                     IKLA"
     gene            complement(3280..3759)
                     /locus_tag="VF182_00890"
     CDS             complement(3280..3759)
                     /locus_tag="VF182_00890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX6435653.1"
                     /translation="MAIEPQELRRVMGHFATGVTVITTQDKDGNPNGLTANAFMSLSL
                     DPPLVLISVDKSAQCYACFNLQNGFTVNFLSEEQEDVSRRFATKGIDKFAGLQWHPGA
                     NGVAILDAALGYVECKITQCYDGGDHTIVVGEIVNANATDRRPLLFFKGKYQRLPDS"
     gene            4054..4617
                     /locus_tag="VF182_00895"
     CDS             4054..4617
                     /locus_tag="VF182_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435654.1"
                     /translation="MAVQERKAESSFDRYIAEVRAVWGDGKDPQLPHKVAALMEKLFA
                     STSAEDSWMAQIIREGKPSRELYRDAEHGFIQMGHVHQPGHGNTPHDHGPCWVVYGAF
                     SGLTEITKYKRTDDGSQPGRATLKKDELHRLSPGTVKPYLPGEIHSTHEVEGPGVVFR
                     FLSYDLDKIERSRYNMEKETIISVPAH"
     gene            4628..5341
                     /locus_tag="VF182_00900"
     CDS             4628..5341
                     /locus_tag="VF182_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6435655.1"
                     /translation="MSVGNIIFEQIRSSGCLSYLLGCPEKKKCVVIDPELDKTDEYIE
                     LAKFFSSELLYAIDTHTHADHNSACKILREKHGIKVIMHEATDAPYIDLAVKDGDLIH
                     FGNESIKVIYTPGHTQDAMCLLCRDRVFTGDTLLIGGCGRTDLPGGSAEQQYESLRKL
                     SALGDEVRVYPGHDYREAVSTLGDEKQKNTRMLFPSKQEFVQFMTSRKPPLPRKIEGA
                     LEWNRTPIQGTQRGMGEGI"
     gene            5607..7463
                     /gene="recQ"
                     /locus_tag="VF182_00905"
     CDS             5607..7463
                     /gene="recQ"
                     /locus_tag="VF182_00905"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEX6435656.1"
                     /translation="MNRSRSWSTADVLATIRRYWGYSELRPLQEEAMRAALDGRDSLV
                     VMPTGGGKSLCYQVPAELAQRTDIVVSPLISLMKDQVDGLRECGYAAAALYSGMPLHA
                     IQETEEQLAAGRYRLVFVAPERLLTARFLQLIAPLKVRAFAIDEAHCISQWGHDFRPE
                     YRQLAELKTRFPRASIHAYTATATQRVRADIIEQLRLSKPVVLVGTFDRPNLVYRIIP
                     RIDGRAQVLEALRRHPGQAAIVYCLSRNDTESMAEALKTSGLRAASYHAGMDADARRH
                     TQEAFSSETLDVVAATVAFGMGIDRSDVRCVIHAAMPKSIEHYQQETGRAGRDGLEAE
                     CVLLYSAADVLRWESLIEKSAAESNPASESIAAARELLAQMRGLCTTVHCRHRALSEY
                     FGQDYSKSQCGACDVCFGEVQGLTDATLAAQKILSCIARAGQQFGAEHIVDVLLGAQT
                     ERVRRWGHDKLTTYSLMKGTDRKSVTNMIYQLLDEGLLERTRDERPVLKLNDASWSVL
                     RSQRTVQLLQPKTKVRKSRPEEESWKNVDSDLFASLRSLRRELAAERGVPAYVLFSDA
                     TLRDMARAKPRSAAALLSIRGVGERKLADLGERFLEVIAKHCRENRLSVDVS"
     gene            complement(7538..7999)
                     /locus_tag="VF182_00910"
     CDS             complement(7538..7999)
                     /locus_tag="VF182_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6435657.1"
                     /translation="MEIEKKLKELGFEVPTPPTPVANYIASVQVGNILFVGGNTGTVN
                     GKRKYTGKVGDAVTVEQAYEMAQFCALNHLAFIKVALGDLDRVERIVKVTGYVNVAPG
                     FKDMPKVINGESDLYVKLWGERGRHARAAIGVASLGGDAPVETEMIVQVKD"
     gene            complement(8066..9175)
                     /locus_tag="VF182_00915"
     CDS             complement(8066..9175)
                     /locus_tag="VF182_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6435658.1"
                     /translation="MATVKVNNLRKSFGNVCAVAGISFEVEQGEFVTLLGGSGCGKTT
                     TLRLIAGLEENDEGEIVLADQVVSNPAAGKFVAPEKRKLGMVFQSYAIWPHMTVFENI
                     AFPLKVRRLPPDQIREQVEEVLTMVGLPELGSRMASRLSGGQQQRVAIARALACNPSI
                     LLMDEPLSNLDAKLRERMRFELREMQTRFGIATLYVTHDQAEAMVLSDRIIVMNEGRI
                     EQVGTPWDIYEHPQSEFVSDFIGVANLLEAVVEESQAEGCRLKVLGNDRTITIDRQVA
                     NATQALLLIRPENVQLGTFQELPAENTWPTKVELAAYFGDHREYVLRDGDLTIRVKTG
                     PKFVFQRGDSIGAHFSPENIVPVPKHGNPRANAQM"
     gene            complement(9192..>10923)
                     /locus_tag="VF182_00920"
     CDS             complement(9192..>10923)
                     /locus_tag="VF182_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6435659.1"
                     /translation="LKNFVIRLRTVLATGLLILVAFLVLPPFLMLFVGSIWGAPPGMP
                     GRLSLSAYIDAFSSPYTYKVIANSFFLSFVKTTLATIWGVVLAWLVTRTDVPLRRTME
                     VLIPVQFFIPAIFNALAYLMLLNPHSGIVNQLFMGAFGLESAPFNLYSYTGMIFVMAL
                     GSTAFIFLLTYGAFRALDPSLEESARTCGAGTLGTLVRVTLPLVAPAIGGAFILSFIQ
                     GIEAFEVPVLIGTPAGIYVFTNEIYRAITFFEPARYGVATALGITIVLLTFAMVYVQW
                     RIQGDRQYSTITGKGYNPRLVRLKHWRWPAFIFGALYIVLAVVLPIGQLLLSSLQSNV
                     GVLTFESFTLKHYYQAFRDDMVLRALYNTLILAVLAAVGGVTLSVLIAYVTTRLNFWG
                     KRWLELLSWLPWTLPGIVLATGMLWAYLTIPGLWIFYGSLWILFIAYIIKGLPLGVRA
                     LSGTMVQVHQELEDCARVHGASWLRTLWSVVLRLIRPGMLSGAIFFVFIAIRDLSTPI
                     LLYGYGTEVLSIVLLQFWAEKPPQVVSVLAIIMLAVLTGFSAIQRVLLIEKERPEAIE
                     GRVPPEIGRL"
BASE COUNT         2497 a         3105 c         2869 g         2452 t
ORIGIN      
        1 ggtttcgacc cggccagcgc ctcggcgccg ttcgtcgcca cccttgtaga tgtcaccgga
       61 ttgatcattt atttcacggt cgcgaccctg attttgcgag gcagtctctt gtgaaacgcc
      121 gagcgcgttg agatccaacg gcgtgtccca gcacgtttta atcagctatt ttggcggcgc
      181 atagagctgc agcagattgc cttcgctgtc aaagaagtaa aaaccgctgc cgctcggacg
      241 gtcgacggcc ggcgtgctgt gaattcggac gccccaatcc ttcattttct gcttggccaa
      301 ttcaaaatcc tcacgagtta catggaacgc atggtgtgtc gccccttcgc gttcgatgaa
      361 ttcaccatct agtgctttcg gtctctgaaa caacacgacc atactgtcgc cgcagcgaag
      421 atcggcgcgc gagagccctt tgcccatgtc gtcgcgattg agcttcgcta tggtcatgcc
      481 gaggatctcg gtgtaaaatt tcaccgctcg atcgatatcg ttacacggaa ttccaatgtg
      541 ataaacaccc attgtcttta tcatcagcac ctcctgatcc gaagattagc attgacccag
      601 ccgcgccgag gatcacgact cgggaccggc agatttaggc cggcgctacc cagacggtca
      661 aatcgagccc tgtcgctgag aggggcgtgt cgcgtatcgg gattctgcag cgcgattcca
      721 ccctacccga ggcgtcactt cctgcgctac ttatctctaa gccaacttta ttttgtgact
      781 tcctcgaagc gcttgataga ccttctcggc tgttagcggc aggtcagtga ttctcacccc
      841 gcaggcgtca gccaccgcat tggccacggc ggcagccgtc ggcgtattgg ctacctcgcc
      901 gatcgccaga ctgttgtatg gacccgggcc atgcggcaca ttttcaatca ccgaagtttt
      961 taacggcggg atgtccatga tgttgggtat tttgaattcg ccgaaattgg tggtcactat
     1021 cttaccgttg tcgaaaagaa tttcttcttg caacgcataa cccaaaccga acacaatccc
     1081 gccatcgatc tgcccctgat gcatcaacgg attaatcacc ttgcctgtgg tgtgcgccga
     1141 taccatctgc ttgaggatca cctgtcctgt ttcaggatcg acttgcactt cggcgatttg
     1201 cgctgagacc gacgcatcat gcgatttttc cgagctcttg tagtagccct tgactgagat
     1261 cggcgagccc ttggttttaa tcacatcagc aaaactcatc gtgcgtttcg atttacggtg
     1321 aatcacttgg gaatcggcaa gctcgagctc ttctgccgga acctttagct ttgccgcagc
     1381 cgcagcaaat atttcttgcc gcgccttcac ccctgcttca taggacgcgt tgccgtagac
     1441 ccttgtggcg cggctgccgc cgacaccacc atcgctggga acggttgtac tgtcgagctg
     1501 cgccagctgg atttcaccgg ccggcacacc gagttcctgg ccgacgcact cgacaataac
     1561 cgtcagaacg ccggcgccct gatccaccac cggcgaggag acttttacct tgccgtcttc
     1621 ttcgattttg acgaagacgt tgccttcgcc gccgctcggg ctccactctg aaaaagccag
     1681 accgcgcccg atattttttg gccggggctt gctgtatccg gacatctcga tcgctttctc
     1741 gagcgcttcc acacctctga tatgtgacac gtggccaccg atcggagttt cgtcgccgtc
     1801 gtgcatcaaa tttttgcgct tgaactcggc cggatccatg ccgagcttgc gcgccacctc
     1861 atccatctgg ctttcgttgg cgaagaatcc ctggggatcg ccgggcgcgc gcatgtgccc
     1921 gcagggaatc ttattggtgt aaaccatccg ttcttcgatc aaacagttgg cgatcttgta
     1981 cggcccagcg caagtcgatg cgccgatcaa ataccccgcc ggcttcatcg atccgtaggc
     2041 gccgctatcg aagatgaaat caagatgatg ggcgacgata gcgccgtctt ttttcacgcc
     2101 ggttttggcc ctgatgatcg cggcatgacg cggatttccg gcagtgagct cttcgccgta
     2161 ttccatcacc attttcaccg gctggccgct cttgagcgaa aggaaatagg caaccggtac
     2221 atccatgaac ccgcccttgc cgccaaagtc gccgccgata tgcatcggat gaaacaccag
     2281 cttttcctta tcgataccaa tgcaattgga gagctggttg cgaaccgcga acggtgtctt
     2341 actgcacgac caaatgttgg cgccgccgtc cgaatcggcg ctgacgacgc aagcgtgcgg
     2401 ctcgagatag gactgatgcg tgacctgggt cgtgaaggtg ttttcgacga tcacgtcggc
     2461 ttgcctgaag cctgtttcga tatcgccctt cccccacttt aaataagcaa agacgttgct
     2521 gacttgttcc accggcacag gaaggccttt ataggaaagc agatccgggt ggagaatcgg
     2581 cgcgtcatct tttattgccg cgagcggatc gaccaaaggt tccatttcat cgtattcaac
     2641 ttcgatcagc tcgagggcct gctccgccgc atgtctggtc tcggcggcaa ccgccgcaac
     2701 tttttcaccg atgaatcgca ccaacccatc cgcgagaatt ggggtgtcgt aaatgcatct
     2761 gccgattctc aatccagcca catcctctcc ggtaatcacc gctttgacgc ccgacatctg
     2821 gcgcgcctta tcggtatgga tcttcttgat ccgaccggag gaaatcgggc tccgcaaaac
     2881 tttgccccaa agaatatccg gcagcctcac atcggcgctg taaagcgctc gtccggtcac
     2941 ctttaactca ccctcgatgc gcggtgttgg ccgccctaca acctgggtca ttacttacct
     3001 ccaagaccgt tcttcaccag tggctggttc atacagggtc atttctagac tcgaatgggt
     3061 tccgttgtcc agcgatagcg gagagggctt tcgctgaaca cgctgatcga tgcagacaac
     3121 tctttcggaa tcgatgcgga acgatgtttc tggcgcagtt tattcaaact cgagtcgagc
     3181 gctcaaaccg gtgatcagaa aactttctac agcatggccg ctgagcatga ttgctcggat
     3241 ggagaacttt ccaatgacga ggaatcgccg gagctgaagc taagaatcag gcaggcgctg
     3301 gtactttcct ttgaaaaaaa gcagtggccg tctgtccgtg gcattggcgt tgacgatttc
     3361 accgacaacg atggtatggt cgccgccgtc gtagcactga gtaattttgc actcgacata
     3421 gccgagcgcc gcatcgagaa tcgctacccc attcgcgccg ggatgccact gcaggccggc
     3481 aaacttatcg atccctttgg tcgcaaagcg acgcgacaca tcttcctgct cttcgctgag
     3541 aaaatttacc gtaaaaccgt tttgcaaatt gaagcatgca tagcactgcg cgcttttgtc
     3601 aacgctgatc aagactagcg gcggatctaa tgacagggac atgaaagcgt tggctgtgag
     3661 tccgttcggg tttccgtctt tgtcctgagt cgtaatcacg gtaacaccag tggcaaagtg
     3721 acccattacc cgccgcagct cttgtggttc gatcgccata attgctttga tgctcgggaa
     3781 ttttttaact gctaccagag tcaacctgat gctgtcaatc agggtcggtc gaaatagcgt
     3841 atcgtgttcg tagaaacgtg atcgagaatt ccgaacgaat acgaacacgt aacacaaaaa
     3901 cggatcgaac gaagcgatcg atgcttcgac aagctcattg tggaccgaat gatccgttta
     3961 tcatgagcac agtttaagaa tgcatctttt cagcaggatg ctggacgatt cctcacggaa
     4021 catggtaaag atgaaattca aaggaggtgc ccaatggccg tacaagaacg aaaagcggag
     4081 agctcgtttg acagatatat cgccgaagtt cgcgccgtct ggggcgatgg caaagatccc
     4141 cagctgccgc acaaggtcgc ggcattgatg gaaaagttgt ttgccagcac cagcgccgag
     4201 gattcttgga tggcgcagat cattcgagag ggcaagccgt cgcgcgagct ctatcgagat
     4261 gctgagcacg ggttcatcca aatgggccat gtgcaccaac caggacacgg taatacgccg
     4321 cacgatcatg gtccatgctg ggtggtttac ggcgctttca gcggccttac tgaaatcacc
     4381 aaatacaaac gcaccgacga cggctcacag ccaggcagag caactcttaa aaaagatgaa
     4441 ctccatcggc tatctcccgg gacggtcaaa ccctatctgc ccggcgaaat tcattcgaca
     4501 catgaggtcg aaggccctgg cgtggtattt cgatttctca gctacgattt agataaaatc
     4561 gagcgcagcc gctacaacat ggagaaagag accattattt ccgttcctgc tcactgaaag
     4621 gttgaacatg agcgttggca atatcatctt cgagcagatc aggtcgagcg gctgcctatc
     4681 gtatctcctg ggttgccctg agaaaaaaaa gtgcgtcgtc atcgatcctg agctcgacaa
     4741 gaccgacgag tatatcgagc tggctaagtt tttttcttcg gaactgcttt acgccatcga
     4801 cacccatacc catgccgacc ataactccgc ctgcaagatc ctgcgcgaga agcatggcat
     4861 caaggtgatc atgcacgaag ccaccgatgc gccctatatc gacctggcgg tcaaagatgg
     4921 agatcttatt cattttggca acgagtcgat caaagtgata tacacccccg gacacactca
     4981 agacgccatg tgccttctat gccgcgaccg cgtttttacc ggcgatacgc tcctcatcgg
     5041 cggctgcggc aggaccgact tacccggcgg cagcgccgag caacaatacg agagcttgcg
     5101 caagctgagc gctctcggcg acgaagttcg ggtttatccc ggccatgact atcgcgaagc
     5161 cgtttcaact ctgggcgatg aaaagcaaaa aaacacgcgc atgctgtttc cgagcaaaca
     5221 ggagtttgtg cagttcatga cctcgcgcaa accaccgttg ccgcgaaaaa tcgaaggcgc
     5281 tctcgaatgg aaccgcacgc ctatccaagg cacccagagg ggtatgggcg aaggaattta
     5341 gcaggctgtg aaaaagactc ctatgcttcg ttgcgttcgc cagactcgct tcaacgtact
     5401 gaagcatacg cctcagctca tcgaacttcg cgcgcctcgc ctatgagatc tttgagcagc
     5461 ctgctagtct ttgagaagtc gtaacagcat ctgagaatca tcatcacttt gctgtaagag
     5521 ctgtccccgt tctctcgaat tgtttttggt gacttctggt acctttttag catctcgttg
     5581 attgatttct atccttccga aaaaacatga accggtccag aagctggtcg acagctgacg
     5641 tcctcgccac gattcgccgc tactggggat actccgagct acgcccattg caggaggaag
     5701 cgatgcgggc cgctctcgat ggccgcgatt cgctggtcgt catgcctaca ggaggcggca
     5761 agtcgttgtg ctatcaagtt cccgccgagc ttgcccaacg caccgatatt gtggtctcgc
     5821 cgcttatctc cttgatgaag gatcaggtcg acggcctgag ggagtgcggc tatgcggcgg
     5881 cggcactcta cagcggcatg cctctgcacg caatccagga aaccgaagag cagcttgccg
     5941 ccggacgtta tcggttggtg tttgtcgcgc ctgaacggct attgacggcg cgatttctcc
     6001 aactcatcgc gccgctcaaa gttcgcgcct tcgccatcga tgaggcccac tgtatcagcc
     6061 aatggggtca tgacttccgc ccggaatatc gccagctggc cgaactcaag acgcgttttc
     6121 cgcgcgcgag cattcacgca tacaccgcga ccgccacgca gcgcgtgcgc gccgacataa
     6181 tcgagcagct tcggcttagt aagccagtgg tgctggtcgg cactttcgat cgtcccaatc
     6241 tggtctaccg gatcattcca cgcatcgatg gacgggctca ggtgctcgaa gcgttgcggc
     6301 gtcatcccgg gcaagcggca atcgtctact gcctgagcag gaacgatact gaatccatgg
     6361 ccgaggcgct aaagaccagc ggcttgcgcg cggcttctta ccacgccggt atggacgcag
     6421 atgcgcggcg ccatacccag gaggcgtttt cctcagaaac gttggacgtg gtcgcggcga
     6481 cggtcgcctt cgggatgggg atcgatcgca gcgacgtgcg ctgcgtcatt cacgccgcca
     6541 tgcccaaatc catcgagcat tatcagcagg agaccggccg cgccgggcgc gacggcttgg
     6601 aagcggaatg cgttctccta tattcggcgg ctgacgtgct ccgttgggaa tcgctgatcg
     6661 aaaagagcgc tgccgagtcc aacccagcct cggagagcat cgccgcagcg cgagaactcc
     6721 tcgcacagat gcgcgggtta tgcaccactg ttcattgccg gcaccgtgcc ttgtcggaat
     6781 acttcggcca agattattcc aaatcgcaat gcggtgcctg tgacgtctgc ttcggcgagg
     6841 tccaaggttt aaccgatgcg acgcttgcgg cgcagaaaat tttgtcctgc atagctcgag
     6901 caggacagca attcggcgcc gaacacattg tcgacgtgct cctgggtgcg caaaccgaac
     6961 gggtgcgacg ctggggtcat gacaaactga ccacctattc tttgatgaag gggactgacc
     7021 gcaagtcggt caccaacatg atttatcagc ttctcgatga gggtctgctc gaacgaaccc
     7081 gcgatgagcg gccggttctc aaactcaacg atgcgtcgtg gtcggtacta cgcagccagc
     7141 gcacggttca actgctgcag ccaaaaacca aggttcgtaa gagccgtcct gaagaagagt
     7201 cttggaagaa tgttgacagt gatctgttcg cgagcctgcg gagtctacgg cgcgaactcg
     7261 ccgccgagcg cggcgttccg gcatacgtgt tatttagcga tgccaccctg cgtgacatgg
     7321 cgcgcgcaaa accacgatca gccgctgcgc tgctgagcat ccgcggcgtc ggtgaacgca
     7381 agctggccga cctgggggag cgttttctcg aagtgattgc gaagcattgt cgagaaaacc
     7441 gcttgtcggt ggatgtcagt tgagtttccg gaaaccaacg cgtgaacatg ccgtggaaaa
     7501 gcgtcgccgc atccaggcat ttttgcgttt tgccggccta atcttttact tggacgatca
     7561 tctcagtttc caccggagca tcgccgccaa gagacgccac gccgatggct gctcgagcat
     7621 gccgaccgcg ctcaccccat agcttaacgt atagatcgga ctcgccgttg atgactttag
     7681 gcatgtcctt gaatccgggc gcaacgttta cgtagccggt gaccttaaca atgcgctcga
     7741 cgcgatcaag atcgcccagt gcgaccttga taaacgccag gtgattgagg gcgcagaatt
     7801 gcgccatctc gtaggcctgc tcgactgtaa ccgcatcgcc gactttaccc gtgtacttcc
     7861 tttttccgtt gacggtgccc gtattgccgc ccacaaacag gatattgccg acctgtacac
     7921 tcgcgatata gtttgccacc ggcgtgggtg gtgtcggcac ctcaaaacca agttctttta
     7981 gctttttttc aatttccatc tttcctcctg tatcaagtta gttgagaatt tcctttggta
     8041 tttcgttagt aagctaatcc aatttttaca tttgcgcgtt cgcccgtgga tttccgtgtt
     8101 ttggcacggg cacaatattc tccggcgaga aatgggcgcc gatcgaatct cctctctgaa
     8161 aaacgaactt cggccccgtc ttgactcgga ttgtcaaatc accatcgcgg agaacatatt
     8221 ctcggtggtc gccgaaatag gccgccagct ccactttcgt tggccacgtg ttttccgcag
     8281 gcaattcttg aaatgttccc agctgaacat tttccggccg aatcagcagc aatgcctgtg
     8341 tcgcgttggc gacctgtcgg tcgatggtta ttgtccgatc gttccccagc actttcagtc
     8401 gacacccctc cgcctgcgac tcttcgacga ccgcctccag taaattggcc acgccgataa
     8461 aatcgctgac aaattccgac tgcggatgct cgtatatgtc ccacggtgta ccgacctgtt
     8521 cgatacgtcc ttcgttcatg acgatgattc gatccgaaag caccatagcc tcggcttgat
     8581 cgtgagtcac gtaaagggtc gcgataccga agcgagtttg catttcccga agttcaaatc
     8641 gcattcgttc ccggagcttg gcgtcaagat tactaagggg ctcatccatg agaagaatcg
     8701 aaggattgca cgctaacgcg cgcgcaatgg ctacgcgctg ttgctggccg ccgcttagcc
     8761 ggctagccat tctactcccg agctccggta agccgaccat cgtgagaacc tcttcgactt
     8821 gctcgcggat ttgatcagga ggtagccgac gaacctttaa ggggaatgca atgttctcaa
     8881 aaaccgtcat gtggggccag atagcgtagg actgaaagac cattccgagt tttcgttttt
     8941 ccggtgccac aaacttgccc gcggcggggt tgcttacgac ttggtcggct aaaacaattt
     9001 ccccttcatc gttctcctcc agtcccgcga ttaaccgcaa cgtggtcgtc ttgccgcatc
     9061 cgcttcctcc cagaagtgta acaaactcgc cctgctcaac ttcgaagctt atgcccgcca
     9121 cggcacaaac gttaccgaag gattttcgta gattatttac tttgaccgtc gccatagctt
     9181 tacaaagttt tttagagccg gccgatttcc ggagggactc taccctcgat cgcctccggg
     9241 cgctctttct cgatcaacaa aactcgttga atagcagaaa agcccgttaa cacagccaac
     9301 atgataatcg ccaacacact gacaacctgc ggcggttttt ccgcccaaaa ttgcagcaaa
     9361 acgatgctga gcacctcggt gccatatccg tacagcagaa tcggcgtact aagatcacgg
     9421 atagcgatga agacaaagaa gattgcgccg gagagcatgc cgggtctgat caagcgcagt
     9481 acgacagacc aaagcgttcg cagccaactt gcaccatgga cccgagcaca atcctcaagt
     9541 tcttggtgaa cttgcaccat cgtgccactg agggcacgga cgccgagcgg tagccctttg
     9601 atgatatatg caatgaatag aatccaaagc gacccataga agatccacag tccgggaatc
     9661 gtgagatacg cccatagcat accggtagcc agcacgatcc ccggaagcgt ccacggtagc
     9721 cagctcagca gttcgagcca tcgtttgccc caaaaattca ggcgagtcgt cacgtaggcg
     9781 ataagcacgg acaaggtgac gccgcctaca gccgcgagga ccgcgagtat gagagtgtta
     9841 tagagcgctc gcaaaaccat gtcatctcga aatgcttgat agtagtgttt gagcgtgaag
     9901 ctctcgaacg tgagcacgcc gacgttcgac tggagcgatg aaagcagaag ttggccgata
     9961 ggcaaaacaa ccgcaagtac gatatacagt gctccgaata taaaagcggg ccatctccag
    10021 tgttttagac gcacgagcct gggattgtag cctttaccgg ttatggtgga gtactggcga
    10081 tcgccttgaa tgcgccattg cacatagacc atggcgaacg tcagtaacac aatagttatt
    10141 ccgagagccg ttgcgacgcc gtaccgcgcc ggctcaaaaa aggttatggc gcggtatatt
    10201 tcattcgtga acacatagat tcctgcgggt gtgccgatta agacaggcac ctcaaacgcc
    10261 tcgatgcctt gaatgaacga caagatgaaa gcgccgccaa tagccggagc gacaagcgga
    10321 agcgtcactc gtacgagcgt tcccaaagtg cctgcgccgc acgtacgcgc gctttcctca
    10381 agggacggat ctaacgctcg aaacgctcca taagttagga ggaagatgaa cgccgtggac
    10441 cctagcgcca tgacaaaaat catcccggta tacgaataaa ggttgaaagg cgccgattcc
    10501 aaaccgaacg cgcccatgaa cagttgattg acgatgccgc tgtgaggatt gagcagcatg
    10561 agataagcca gagcgttgaa gatcgccgga ataaagaact gaaccggtat gagcacttcc
    10621 atagtgcggc gcaacggaac atcggtccga gtaaccagcc atgcgagaac aactccccaa
    10681 atggtggcca gggtggtctt cacaaacgaa aggaagaaac tattggcaat gactttatag
    10741 gtgtacggcg aactgaacgc atcgatatag gccgagagcg aaagccggcc gggcatacct
    10801 ggaggcgccc cccagatgct accgacgaac aacatcagaa aaggcggcaa gactagaaag
    10861 gcaaccaaga tcaatagacc ggtagctagt acggttctca ggcgaatcac gaagtttttc
    10921 aaa
//
LOCUS       DASUVS010000016         6853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_4806, whole genome shotgun sequence.
ACCESSION   DASUVS010000016 DASUVS010000000
VERSION     DASUVS010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4806"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1090
                     /locus_tag="VF182_00925"
     CDS             38..1090
                     /locus_tag="VF182_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6435660.1"
                     /translation="MTSNFDRSAEATLHLAEESPRPRVGFLGVGWIGRHRLQSILECG
                     AVEIVAVADFDGAVARAAAGHIPGAVAVPSLDALLESELDGVVIATPTALHAEQATAA
                     LEKHTAVFCQKPLGRSADETARVIRAARRANRLLAVDLSYRFVRGIQKIKQMISAGEI
                     GAVYVADLVFHNAYGPDKAWYYDRKFSGGGCLIDLGIHLIDLALWMLDFPEVQNVSSR
                     LFSQGRPLQQLPGALEDYTLARLDLASGATVNLACSWRLPAGRDAVIRASFFGPAGGL
                     SIQNVGGSFYDFCAQRLQGTNRVILDQPPDPWGGRAAVAWSERLRADKSFDAQIERAY
                     PVAEVIDEIYRQNIAG"
     gene            1109..2239
                     /locus_tag="VF182_00930"
     CDS             1109..2239
                     /locus_tag="VF182_00930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6435661.1"
                     /translation="MLLPMRPILMTADPIGGVWQYALELCRQLAESDVNVVLATMGRR
                     LERSERLSVCGLQNVELCESAHKLEWMREPWSDVKAAGQWLLDLESRFRPSVIHLNQY
                     SHGAISWKVPALVVGHSCVYSWFEAVKRAPPGAEWADYVQAVRLGLQGADLVTAPSRS
                     MLASLIKHYGPFVAAEPINNGCSGARFIPGSKQDFIFTAGRLWDEAKNIGILNSVCGK
                     ISWPIYAAGESVAPDGTPALVDGPILLGRLDAEMLTQWFSRAAIFILPARYEPFGLSA
                     LEAALSGCALVLGDTESLREIWDDAALFVGPEDPEHIRETLVGLIANPSRREDFARRA
                     RKRAERFTPDRMARAYLEVYAKLLQAQPSVLFEEMTRAEYRS"
     gene            2236..3375
                     /locus_tag="VF182_00935"
     CDS             2236..3375
                     /locus_tag="VF182_00935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6435662.1"
                     /translation="MNIALFYHSLVSDWNHGNAHFLRGIAWELQRRGHTLRIFEPAGG
                     WSLTNLSREHGQAPLDLFAKIYPGLRSTFYDPATLDLDKALDRIELVIVHEWNDPAWV
                     GRIGAHRRTRGDYRLLFHDTHHRSVTEPEKMAAYDLADYDGALVFGQAIAEIYRSRNW
                     ARTVWVWHEAADTRVFMPFSDMQKEGDLVWIGNWGDEERSAELHEFLLEPVKALGLKA
                     RVYGVRYPEDALQALSDAGIEYGGWLANFEVPAVLARFRCTVHIPRRPYVSALPGVPT
                     IRPFEALACGIPLVCAPWEDSEHLFSPGRDYLIARDSAAMKRQLQRLLVDRQLAGALS
                     AHGRQTILHRHTCAHRVDELIDIYHAIDAPIPSGQAMRGASEKRL"
     gene            3372..4445
                     /locus_tag="VF182_00940"
     CDS             3372..4445
                     /locus_tag="VF182_00940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6435663.1"
                     /translation="MKISLFGSSLLSAYWNGAATYYRGMLSALFARGHQIVFYEPDAW
                     DRQTHRDLTDTPYAQTVVYPVDRDSSVVEMVKKAARESDLLIKASGVGVFDALLEQAV
                     IDLRRAGTMAAFWDVDAAATLERIGQDAADPFRALIPLYDLILTYGGGDAVVQSYRRL
                     GAQRCFPIYNALDPSTHYPAPPDPNYDGALSFLGNRLPDRESRVSEFFFKAAQANPRM
                     RFVLGGSGWEDTARAYPNVAYLGHVYTAAHNAFNSSPMAVLNVCRESMARCGYSPPTR
                     LFEVAGAAGCLITDAWQGIENFLEPGRECLVASDGDEVGEHLRVLTQVQAKQIGQAAL
                     RRIVGEHTYAHRAAELESLLQDL"
     gene            4442..5521
                     /locus_tag="VF182_00945"
     CDS             4442..5521
                     /locus_tag="VF182_00945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6435664.1"
                     /translation="MNTDPLNIVILGLTITSSWGNGHATTYRALVRGLEQRGHQVLFL
                     ERDMPWYAAARDQGDARQGRTVLYRSLEQLKKRFHGAVRDADLVVVGSFVPEGIELGE
                     WVVETARGVTAFYDIDTPVTLADVERGDSFYLSAALIREYQLYLSFTGGPILKRLERQ
                     YGAPMARTLYCAVDADLYRPLALPKRWDLGYMGTYSADRQQVLEELLLEPAGRCPDAR
                     MVVAGPQYPGSIEWPANVERIEHLAPCAHPAFYNQQAFTLNLTRANMIHAGYSPSIRL
                     FEAAACGCPIISDWWEGLGDFFKFDEEILVADSPEKTLEYLFGLSAQERELIGQRARD
                     RVLSGHTAPRRAAELEGYARQLLGS"
     gene            5604..6128
                     /locus_tag="VF182_00950"
     CDS             5604..6128
                     /locus_tag="VF182_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435665.1"
                     /translation="MGVRRYSNLVEINATPSRLSPSAFVLDNQLPELPLDEPLPEEPL
                     LDPVLAPLLEEPPEDEPLPPVPDDLPVEESPAPNELPDEDPLEDDLSLDGLRPDEEAP
                     LPDELFSLEEAPLDDDPLPEASTPNALAVLSSSRPVACIPLSFWKARRACCVFGPMTP
                     SAGPGSCPLFFSAS"
BASE COUNT         1290 a         1957 c         2056 g         1550 t
ORIGIN      
        1 ccccgggtgc tttgcatcaa caacacggaa ggaatgaatg actagcaact tcgatcgatc
       61 ggccgaagcc accttgcacc tggctgagga atccccacgg ccgcgagttg gatttctcgg
      121 cgtcggctgg atcggccgcc atcgcctgca atcgattctc gaatgcggcg cggtcgaaat
      181 cgtcgccgtg gccgatttcg atggtgccgt cgcccgcgcg gctgccgggc atattcccgg
      241 agcggtggcc gtgccgtcgc ttgacgcgct gctcgaatcc gagctcgacg gcgtggtgat
      301 cgcgacaccg acggcgttac acgccgagca agctacggcg gccctggaga agcacacggc
      361 cgttttttgt caaaagcctt tggggcgaag cgccgacgag acggcgcgag ttattcgagc
      421 tgcgcggcgg gcgaaccggc tgttggccgt cgatctgtct tatcgctttg tccgtggtat
      481 tcaaaaaatc aaacagatga tcagcgcagg ggagattggc gccgtgtatg tcgctgatct
      541 tgtgtttcat aatgcctatg gaccagacaa ggcgtggtat tacgaccgca agttttccgg
      601 cggcgggtgc ctcatcgacc tcggcattca tctcatcgat ctggcattat ggatgctcga
      661 tttccctgaa gttcagaacg tctcgagccg actgttcagc cagggcagac cgctgcagca
      721 attgccggga gctctcgaag attacacgct ggcgcgcctg gatctggcga gcggcgcgac
      781 cgtcaatctt gcctgttcct ggagattgcc ggcgggacgc gacgctgtga ttcgcgcatc
      841 tttctttggc cccgcaggtg gcttgtccat tcaaaacgtc ggcggctcat tttacgattt
      901 ttgcgcccag cggcttcaag gcactaaccg agtgattctc gaccagcctc cagacccatg
      961 gggcggccgg gccgcagtgg catggagcga gcggttgcgc gccgataaaa gcttcgatgc
     1021 gcagatcgaa cgagcttacc cggtggctga agttatcgat gaaatttacc ggcagaacat
     1081 tgccggctga gggagggaaa tcccgcgagt gttgttaccg atgcggccga ttctgatgac
     1141 cgccgatccc atcggcggag tttggcaata cgcgttggag ctttgccggc aattggccga
     1201 gagcgacgtc aacgtcgtgc tggcaaccat gggcaggcgc ctagagcgga gcgaacggct
     1261 ttcagtttgc ggtttacaga acgttgagtt gtgtgagagc gcccacaagc tcgagtggat
     1321 gagggagcca tggagcgatg tcaaagccgc cgggcaatgg ctgctcgatc ttgaatcgcg
     1381 ctttcgaccg agcgtgatcc atctgaatca atacagccat ggcgccattt cctggaaggt
     1441 tccagctttg gtggtcggcc actcgtgtgt ttactcgtgg tttgaagccg tcaaacgcgc
     1501 gcctcccggc gcggaatggg cagactacgt gcaggctgtg cgattgggtc tccaaggcgc
     1561 cgatctagtc accgcgccga gccgctcgat gttggcatcc ttgatcaagc attatggacc
     1621 gtttgtcgct gcagagccga tcaacaacgg ctgcagtggc gcccgcttta ttccggggag
     1681 caagcaggac ttcatattta cggccggtcg cctttgggac gaggccaaga acatcggtat
     1741 cctgaactcc gtctgcggaa agatttcctg gccgatttat gccgctggag agagcgtcgc
     1801 gccggacggc acaccggcgc tggtcgatgg gccgatttta ttaggccgcc tcgatgcgga
     1861 aatgctcaca cagtggttta gccgcgcagc gatttttatt ttgccggcgc gctacgagcc
     1921 atttggttta tcagcattgg aggcggcgct ctctggctgc gccctggtgt tgggagacac
     1981 tgaaagtctg cgggagattt gggatgacgc cgctcttttt gttgggccgg aagatcccga
     2041 gcacattcga gagacgctcg tcggattgat agcaaatcca tcccggcgcg aggacttcgc
     2101 ccgacgcgcg agaaaacgcg cggagcgttt tacgccggat cgaatggccc gggcctatct
     2161 cgaggtctat gcgaaactgc tgcaagcgca accaagcgtg ctcttcgaag aaatgacgcg
     2221 cgccgaatac cgatcatgaa tatcgctctt ttttaccatt cgctggtttc cgattggaac
     2281 catggcaatg cgcacttctt gcgcggcatc gcctgggaac ttcagcggcg cggccatacg
     2341 ctgcgaattt tcgaaccagc cggcggctgg agcttgacga atttatcgcg agagcacggc
     2401 caagcgccgc tcgatctctt cgctaaaata taccccggct tgcgcagcac cttttacgat
     2461 ccggcaactc tcgatctcga caaggccctg gaccgcatcg aactcgtgat cgttcacgaa
     2521 tggaatgacc cggcctgggt cgggcgaatc ggcgcgcacc gcagaacccg cggcgattac
     2581 cggttattgt ttcatgacac gcaccatcga tcggtgaccg agcccgagaa aatggcggcc
     2641 tacgatttgg cggactacga tggcgctctt gttttcggtc aagccatcgc cgagatctat
     2701 cgttcccgca actgggctcg aaccgtttgg gtgtggcatg aagcggccga cacgagagtt
     2761 ttcatgccgt tctccgatat gcaaaaagag ggcgatctgg tttggatcgg caactggggc
     2821 gatgaagagc gttcggccga attgcatgaa tttcttctcg agccggtaaa agcgctgggt
     2881 ctcaaggcgc gcgtttacgg cgtccgctat cccgaagatg cgttgcaagc gctttcagat
     2941 gccggcatcg aatacggcgg ctggttggca aattttgaag tccccgccgt actggctcgc
     3001 ttccgatgca cggttcacat cccgcgccgg ccttacgtca gcgctttgcc gggcgttcca
     3061 accattcgtc cgttcgaagc gctggcctgc gggattcctc tggtttgcgc gccctgggaa
     3121 gacagcgaac atctattcag ccccggaaga gattatctga tcgcccggga tagcgccgcc
     3181 atgaagcggc aattgcagcg cctgctcgtt gatcgtcaac tggccggcgc attatctgcg
     3241 cacgggcgcc agacaatcct ccaccgtcat acctgcgccc atcgcgtgga cgaactgatc
     3301 gatatctatc atgcgatcga tgctccgatc ccgtccggtc aggccatgcg cggcgcgtcc
     3361 gagaagcggc tatgaaaatc tcgctttttg gctcgagcct gctgtcggcg tattggaacg
     3421 gcgcggcaac gtattaccgg ggaatgttaa gcgcgctttt tgcgcgaggc catcagattg
     3481 ttttttatga gcccgatgct tgggatcgcc agactcatcg ggatctcacc gacactccct
     3541 atgcgcaaac ggtggtgtat ccggttgaca gagactcgag cgtcgtcgaa atggtcaaga
     3601 aggccgcccg tgaatcggat ctgctcatca aggcgagcgg cgtgggcgtt ttcgatgcgc
     3661 tgctggaaca ggccgttatc gatttgcgcc gcgccggcac catggctgct ttttgggatg
     3721 tcgatgccgc ggcgacactg gaacgaattg gccaggatgc tgccgatcct tttcgcgcgc
     3781 tgattccact ctatgatctg atccttacct atggcggtgg cgatgcggtg gtgcagagct
     3841 atcgtcgcct gggcgcgcag cggtgtttcc cgatttacaa tgccttggat ccaagcacac
     3901 attatcccgc gccgcctgat cctaactatg acggcgcgtt gagcttcctc ggcaatcgcc
     3961 tgcccgatcg cgagtcgcgg gtatctgaat tttttttcaa ggcggcccaa gccaatccgc
     4021 gcatgcgctt tgtccttggc ggcagcggtt gggaggatac ggcgcgagcc tatcccaatg
     4081 ttgcctatct tggccacgtt tataccgcgg cacataacgc cttcaattca tcgccgatgg
     4141 cggtcttgaa tgtttgccgt gaaagcatgg cgcgctgtgg ctattctcca ccgacccgcc
     4201 tgttcgaggt tgccggagcc gccggctgtc tgattaccga cgcatggcag ggcatcgaaa
     4261 attttctcga accaggccgc gaatgtttgg ttgccagcga tggcgatgaa gtcggcgagc
     4321 acctgcgcgt tttgacccag gtccaagcta aacagattgg tcaagctgct ttgcgccgaa
     4381 tcgtcggcga gcatacttat gcgcaccgcg ccgctgagct ggagtcgttg ctgcaggatc
     4441 tatgaatacc gatccgctca atatcgtgat cctgggtctt acgatcactt cgtcctgggg
     4501 caatggacat gcgacgactt accgggctct ggtgcgcggg ctcgaacagc gcgggcatca
     4561 ggttttattt ctcgagcgcg atatgccctg gtatgccgcg gcgcgcgatc aaggcgatgc
     4621 gcgccagggc cgaacggttc tttaccgcag tttggagcaa ctcaagaaga gatttcacgg
     4681 tgcggttcgc gatgccgatc tggtcgtcgt gggctcgttt gtgccggagg gaattgagct
     4741 cggcgagtgg gttgtggaaa ctgcccgcgg ggtgacggcc ttttacgata tcgacacgcc
     4801 ggtgactctg gctgatgttg agcgcgggga ttccttttac ctttccgccg cattgatccg
     4861 tgaataccag ctatatctct cctttaccgg cggcccgatt ctaaagcgat tggaacggca
     4921 gtatggcgcg cccatggcgc gaacgctgta ttgcgccgtc gatgccgatt tataccggcc
     4981 cctggcgctg cctaagcgct gggacttggg atatatgggc acttacagcg ccgatcgcca
     5041 acaggttcta gaagagttgc tgctcgagcc ggccggtcgc tgccctgatg cgcgtatggt
     5101 ggtcgccggc cctcagtatc ccggctctat cgagtggccc gccaacgtcg agcgcatcga
     5161 gcatctggcg ccgtgcgcgc atccggcttt ttacaaccag caagcgttta cccttaatct
     5221 gacgcgggcc aacatgattc acgccggtta ttcaccgagt attcgcttgt ttgaagcggc
     5281 tgcctgcggg tgtccgatca tcagcgattg gtgggagggg ctgggtgatt ttttcaaatt
     5341 cgacgaggag attctcgtcg ctgattcacc ggaaaagact ctcgaatacc ttttcggtct
     5401 aagcgcgcaa gagcgtgaac tgatcggtca acgagcccgc gaccgcgtgc tatcgggtca
     5461 tacggcgccg cgccgggccg cagagctcga aggctatgcc cgccagctcc tcggctcgta
     5521 acctcgttgg gagacactgc tgagcagatg cgaacgcaag aacagggagg cctttcgtgt
     5581 gacctccctg ttcaataggc tgattgggcg tccgccgata ctcgaatcta gtagagataa
     5641 acgcaacgcc atcgcgctta tcgccttctg ccttcgtgct cgacaatcag ctgccggagc
     5701 taccgctcga tgagccactc ccggaggaac cgctgctcga tccggtgctc gcaccgctct
     5761 tggaagaacc gccggaggat gagccgctgc cgccggttcc cgatgatctg cccgtcgaag
     5821 aatccccggc ccccaatgag ctgccggatg aagacccgct ggaggatgac ttgtcgctgg
     5881 acgggctccg ccccgacgag gaagcgccac ttccggacga gcttttttcg cttgaggaag
     5941 ctccgctgga cgacgacccg ctcccggaag catctacgcc caatgcgctt gcggtcttat
     6001 cgtcgagccg tccggttgct tgcataccgc tttctttctg gaaggcgcgc agggcttgct
     6061 gcgttttcgg tcccatgacg ccgtctgccg gtcctggatc gtgcccctta ttttttagcg
     6121 cttcctgaac ctgcttgatg ttttcactgg attgtccgct cattccgcgc gcgctcggtt
     6181 gaccactagc gctgccgctt cgcatcgcgc tgccagaacg acttgagcct gtggcagagc
     6241 cgctttcctg gctcgaactc gaggacgatg cgctgcttcc cttttggctc gccggcttat
     6301 ccgaactttc ctgtcctgaa ggccctgtcg atccgccgga gcctttcgtc tgtgaccaca
     6361 ccaggttgcc gttgagtgcg atcgtaccgc taaaaataat gcctgataaa attaatagtt
     6421 ttcgctgcat ccgttcttct cctttctgtg aattgtggcg ttgcgtccgt ttatgtttgt
     6481 gcaacggata tgccaggcgt ttcgacgaaa ttgcgttgca ttctcaacgg cgcctttggg
     6541 gaaggcggaa tgcgagcgcg gattctgtcc caaacgcgaa taaagccgat gagagcgtct
     6601 tccgcaactg ggacgagtaa actagataaa ctagtctaca ttgacaatga ggcgaagagc
     6661 gcgatgggtc cttgcctgca tgatcgtgac ttgctggtaa tggtcagatt gtgaggaaat
     6721 cggcaagcgc cgaggcataa tcggtggatc ggtgcgttta ctgaagggaa atagccgtca
     6781 ccctctcggc gatttagtga gcagtagcga ataaggaact tggccgaaca atccagctag
     6841 gaacgcatat cgc
//
LOCUS       DASUVS010000017        13948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_5301, whole genome shotgun sequence.
ACCESSION   DASUVS010000017 DASUVS010000000
VERSION     DASUVS010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13948
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5301"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            414..638
                     /locus_tag="VF182_00955"
     CDS             414..638
                     /locus_tag="VF182_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435666.1"
                     /translation="MKNYILLLALLAVTQSSCAFLGGAATGAVAAGAGYEIQAKRQMD
                     RLDEDFKNRRISREEYEDRRQQIQKGSIIY"
     gene            647..1339
                     /locus_tag="VF182_00960"
     CDS             647..1339
                     /locus_tag="VF182_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX6435667.1"
                     /translation="MKNSITQIRIIPVAILVTLLSGCVIADEAAPRADTRNDDRGQPS
                     KTRQLDARQADRFYRIMTPLLKNMNNPKSPKEVKIGIVDESEINAANAGGGEFYVTTG
                     LLEKASDEQLRVIMAHEIAHDDLGHVAKLQGLGAGLNIGVILLEQILPGSSAVTPIAG
                     SLIARGYSRNEEYAADRHGVEILQRAGYSKDVLINALNWVTRTSGGGGGGGFLSTHPA
                     TEDRIAQLQKLR"
     gene            1354..1968
                     /locus_tag="VF182_00965"
     CDS             1354..1968
                     /locus_tag="VF182_00965"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEX6435668.1"
                     /translation="MKPQPVITTPRAIFTLAAVSAMPMIYSGCGDLALLGRPALDARS
                     RRENAEFIGTVEEVDHSRGELYLRTQGGQSQIVTYTNRTQIILDGEETAASAIERGDI
                     IEVRMHGSADGRAVADFIRVRERGQTGTTSIEGAVERVLSERSVVELRTSAGALISVY
                     LPQNSPDIIQDEFSRLRSGDFVRFRGSFLGENRFESTGILTHPD"
     gene            1984..2121
                     /locus_tag="VF182_00970"
     CDS             1984..2121
                     /locus_tag="VF182_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HEX6435669.1"
                     /translation="MQFYISLQPLIALIAGILILLIPRLLNYVVALYLIITGVLGLTR
                     M"
     gene            2216..2380
                     /locus_tag="VF182_00975"
     CDS             2216..2380
                     /locus_tag="VF182_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006340381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX6435670.1"
                     /translation="MLSWAITFLIIGLIAGLLGLTGIAGAATQIAWILFVVFLVLFVV
                     SLIMGRRSSV"
     gene            2449..3162
                     /locus_tag="VF182_00980"
     CDS             2449..3162
                     /locus_tag="VF182_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6435671.1"
                     /translation="MNSFPILVAEDNQDLLNLIVKYLELCGWRAAAANTRENFWLQLE
                     RQRPELILLDVTLHVSEATSLLNELKSDGRFTDIPVIAMTANGSMRQKNLCIAAGCDR
                     VLAKPFHLDQLRRLVRCFASNHKNEQKTGGIIMKKPIKHFAAVALLAVALFGCQAMTG
                     RTAGQNVDDARISTAVQTTLTSDKASNFTRIDVDTTNGVVYLNGTVQSAEQKARAEQL
                     AGRVDGVKKVVNNLQVGKS"
     gene            complement(3351..3524)
                     /locus_tag="VF182_00985"
     CDS             complement(3351..3524)
                     /locus_tag="VF182_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435672.1"
                     /translation="MEQPVYAFDCWRLEPRTRLWREQTVRLASPAVVWVVDRRREADL
                     ADPMFRVYAKVAH"
     gene            3523..4668
                     /locus_tag="VF182_00990"
     CDS             3523..4668
                     /locus_tag="VF182_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6435673.1"
                     /translation="MKPAGQEGSEPRQDEDKADLQDLLPTQRALDIRSVSLTGLFVLA
                     TFYTLYFARDFILPILLAWILSSLLAPIVRLFKRVRIPEPLSALFIILALLGILSYGV
                     YSLSDPVAAWIHRAPQVLTDVRAKLQNFIQPVAQVQQTTKEIDKMTSLGKDEEAPAVE
                     LKKPGMGEVLLRRAKDFLLGGGVMLILLYFLLASGDLFLLKLVKILPTMENKKVAVEI
                     YRHIEKDVSTYLTVVTLIYIGFGCVIGSAMYFLGMPNPWLWGVMAAVLGYIPYLGALV
                     GLSTVTVVAILTFDDIYRILSVPVIYFTADTIQANLIFPMALGRRLALNPVVIFIWLI
                     FCGWIWGITGALVAVPLLAIIKIISDQVEQLAPIGEFLGSESKAASP"
     gene            complement(4700..8497)
                     /locus_tag="VF182_00995"
     CDS             complement(4700..8497)
                     /locus_tag="VF182_00995"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX6435674.1"
                     /translation="MSRLLAILYRIALRLGFLLLLLIIAAAIITHTDRFREFVRDKLV
                     TAINDTIVGSLSLGEIDGSLWGNVILHDVVVRHEGDEILAVHELTISYSLFPLIWGRL
                     HIFQLAGSRPTLRLRRDEDNQLNIVQAFSPKQPQSEEPSGLVLSLNSVSLREGHIELR
                     LTGPQPQNYTLENVIVDGRIDARPSTLTIDLSHVAAQLNSPGLPELKIDGALAYRETD
                     GTDRLELANFVVTTGDSRVKLDGKIADLQDTNLDMKIVVERLAPHDVGRVFADWPIRQ
                     DVVGSITVNGPLNALSFDIDLAAAGAKLAGNLELDAAGETPGYRGKINLSGFDLARLL
                     GRNDIGGVASAALEGGGKGLSMADLDAKGVIRIQNAGVGAWALGEFSMDGRMHESVAQ
                     INGNLKGKMGLASWQGEVSLKEKPAYQLSLGVENLDIEKAAGDQKQITGKISLKGTIK
                     GAGLTPSDMVASTDLKILPSTIGPVEVQQGSVIANLAQQRLQILEATARTEDAMLTVQ
                     GDVGLDPKQNGKLQYVVRSANLTPWLVLANQKGSGSIELTGRASGNIADLRSQGTMKL
                     AGLRLQNTTTKSGNIDFDLRLAGTQSLPQGTMKIRLDEIQAGIRLQRLDGSIGLSTKK
                     NYELGIDLKAVDQAKRTHILTGQLDYQPQLMVARLDRVGLSLDDGVWQLSRPATIMIR
                     GDNFDIDGLLLRNRVQQVAINGRFATSGKQDLAVTVEGFPISTVSALLPKPLPMTGTL
                     AVRAQVTGTAADPEINASANLTDSSIGGQSYGGIIGDIAYRSRTADIKLTVRQNADHV
                     LTANGTIPLILRWSPQWQAEATGDMNLRVQSSGLSIAFLNAFSGDAAQSIAGIIALDV
                     TAQGPVTKPILRGTFQLRDGGVALKPLGVQVAAINADGKFDGQTVSLRQISARAKDGR
                     LNGSGILSLKDYSPEDFKISLALSRWPVIETQRYRATVAGTIEVQGPLGKPSLKGQIE
                     VLNAEVRPDLAFLSRGRRPLTRDETIVIVRGRTLEEAQPQENKTARPGENELFKNARV
                     DLSVVVPNQAWIRHPEANVELSGKLRVVKEAGKEMTLVGTITVVRGWIGFQGRRFNLS
                     RGSVEFRGDETLNPALDIVAQHRLQQYQVEVIVTGTLDKPVLTLRSDPTLEQADILAL
                     LLFGKPLNTLNRNEQNTMQQSAAEMAGGFAASKVAGAVSEALGLDRLGLDIGEIDAGG
                     GQVGVRRYIGDRTYVSISQDLSGEAGQEVSIEYQIGPDWKITTSTSTTGSSGVGILWH
                     KRY"
     gene            complement(8499..10325)
                     /gene="bamA"
                     /locus_tag="VF182_01000"
     CDS             complement(8499..10325)
                     /gene="bamA"
                     /locus_tag="VF182_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX6435675.1"
                     /translation="MKFIFCLFSALAVWLLLMCRVMAIQLEQLDSAREWRVDKIKISG
                     NQRFSDSQLLAEMVTQARPWYGLWEERPLFDTVTFATDLDRLRRFYQSEGYYKNLITH
                     DIDPDDTRGLVSLNIYIEENSPVVVSQVLIEVNAESPEHPPLPEKFPFTPGEIFREQK
                     YQDAELFLRNHLMDNGHAHTQTQRRAEVDLEQDQAVVRYSAQPGPKAVFDETEIKGSE
                     QVEPYLIRRELAYQPGEMFSLKKIRESRQQILALDLFSAIAINPRESAEKSTVVPMEV
                     QVTEKPPREISLGFGYSTEEEFRVSLDWRHQNWLGDGRRLSIRGKYSSILVSGAIDFI
                     QPHFFTRQTQAGLNLRHDQEDEETFKRNVSRFSGRLEHRFSPRLLSFLGYRVEYNQLS
                     DVADATPQALGEIRTKGILTGPSLGLVWNSTDDLFKPSQGEIFSFNADQGGGIWGGPY
                     EFFKLTGEAKKYFPIGWDTIFAGRLKLGVADAIGSKKNYPLFERFFAGGQSSVRGYGR
                     RRLGPLNSDDEPLGGLSLIEGSVEVRRPVWRELGGALFLDFGQVSLKSFDIPVNDLQF
                     STGFGLSYTTPVGPLRLDIGFPFNPPRGDRAWQIHFSVGAAF"
     gene            complement(10728..11099)
                     /locus_tag="VF182_01005"
     CDS             complement(10728..11099)
                     /locus_tag="VF182_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HEX6435676.1"
                     /translation="MNAIGARLWAIPEGYIPGWSNGPEPEFTSHETVCLLNTADQDAH
                     VVITVFFSDREPAGPYAITVAARRTKHVRFNDLRDPEKIPLATDFASVIESDIPIVVQ
                     HTRLDSRQAENALLSTIAYRE"
     gene            11456..13066
                     /locus_tag="VF182_01010"
     CDS             11456..13066
                     /locus_tag="VF182_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX6435677.1"
                     /translation="MTVNEPAIQSRACAPPLDESILVALYNGIPEAVIVIEFGSRRIV
                     YWNDGASLLFGYSAQEILQQTTERLYLDKNWFDRIYQTSTPEIEQSGCWRGEWQYRRR
                     DGSLFAAEATCTLLRTDKGAYVVKVIREVTERKEAEKIILELNQRLAQRVVEQTSELM
                     SKTEELQHEEAQRKQVEQILGNLLRNITNYAVLITDADGRVVDWNRTATRILGRESAD
                     MTDQRLSVFFADEEGRQALIQKALEEASDQGTFRDYQLLVRKDGSRFYARVIVFALRD
                     NADGLQGFLILLRDDSQERNLREKLREKEHLAAIGTAASMLAHEIGNPLNGISTTVQL
                     LERFISREGIKAPAHMLATLKDLKSEIDRLTSLLNEFKTIARPQRLNVQPVNLASVIQ
                     ETISGLDMDGKGDVQIVWDCAPDLPPIRGDAAKLKQAFLNLFKNAVEAMPYGGTLSTK
                     GYLSAGSICIDISDTGIGIPDDLKVFDLFSSTKPHGTGLGLFIVQQIILAHDGVVSYA
                     SKVGKGTTFHLTLPGSTTPEGVADTRRN"
     gene            complement(13116..13547)
                     /locus_tag="VF182_01015"
     CDS             complement(13116..13547)
                     /locus_tag="VF182_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEX6435678.1"
                     /translation="MNNRLAKELGATLRRKRSSLLLQEIVGTQNEVEPALEDRESELE
                     ESAQKDRMTRLESSLTERGQILLRQIDEALERIDAGTFGECERCGNDIGPGRLKAMPT
                     AALCIDCATALEKRQRTLGANGDTERLPFKDDELDRALGSE"
BASE COUNT         3100 a         3754 c         3782 g         3312 t
ORIGIN      
        1 aaaaggcttc tcacccgtag tgatgagaag cctttttgca ttctgcagcg acagacttgc
       61 taattctcca gagttcgatc gttacaacgc gcacccgtac gctattgttc ggctggcgcg
      121 aatcggttct tctcactcgc attctaaaaa ccttcgcact aacatatagc cccccaattc
      181 gtctcgactg tgattcagaa ttgcgcgtaa tgaagggaat catcagcacc gtcccacaac
      241 tgcgccgcga ggcgttcgaa ctgttctact ttcaagacga acgagaatcg ggcgcctagt
      301 tttcgttctt atgcatcgtg gcttccttga attgccgggt agaatgcgca gccgtggcac
      361 atccattgct ctttgtacct ttcaacatcg ggtttaaacg aaaggaagct tcaatgaaaa
      421 actacatact attgctggca ttactcgcgg tgactcagtc gagttgtgcg tttctcggtg
      481 gagcggcaac gggagcggtg gccgccggcg caggttatga gatacaggcg aaacgacaaa
      541 tggatcgatt ggacgaagat ttcaaaaata gaaggatatc gcgcgaggag tatgaggacc
      601 gtcgacagca gatccaaaaa ggatcgatca tttattagag attctgatga agaattcgat
      661 aacccaaata cgtatcattc cggttgcgat cttagtaacc ttgctctcgg gttgcgtgat
      721 tgcggacgag gcggcgccgc gggccgatac caggaacgat gatcgcggtc agccgagcaa
      781 gacgcggcag cttgatgcgc ggcaagccga ccggttttac cggattatga cgccgctgct
      841 taaaaatatg aacaacccga agagtccgaa agaggtcaaa atcggcatcg tcgatgaatc
      901 tgaaatcaat gcggcgaacg ctggtggcgg cgagttttac gttaccaccg gactattgga
      961 gaaggccagc gacgaacaat tgcgcgtcat catggcccat gaaatcgcgc atgacgacct
     1021 cggtcatgtg gctaagcttc aggggttagg cgcgggattg aacataggag tgatcctttt
     1081 agagcaaatt ctcccgggca gcagcgcggt gacgccgatt gcggggagcc tcatagctcg
     1141 gggctatagc cggaacgaag agtatgccgc cgatcggcat ggtgtcgaaa ttttgcaacg
     1201 cgccggatac tcgaaagatg ttttaatcaa cgcgctaaac tgggtcactc gcacatcggg
     1261 tggcggcggt ggcggcggct ttctttccac ccatccagcc acggaagatc gcatcgcgca
     1321 gttacaaaag ttgcgataac agaggacgcg cgcgtgaaac cacaacccgt aataaccacg
     1381 cctcgcgcta ttttcacgct agctgcagtt tcggcaatgc ccatgatcta ctcgggctgt
     1441 ggagatcttg cgttgttggg gagacccgcg ctcgacgcgc gctcccggcg cgagaacgcc
     1501 gaatttatcg ggacggtgga ggaggtggat catagccgcg gcgagcttta tttacgcacc
     1561 caaggagggc agagccagat cgttacctac acaaaccgca cccagatcat cctcgatgga
     1621 gaggaaactg cggccagtgc gatcgagcgc ggtgacatca tcgaggttcg gatgcatgga
     1681 agcgccgacg ggcgcgcggt ggcggacttc atccgtgtgc gtgaacgggg acagaccggc
     1741 accactagca ttgaaggagc ggttgagcgg gtgctcagtg aacggagtgt agtcgagctg
     1801 cgtacttccg cgggcgcttt aatcagcgtg tacctgccgc agaattctcc ggacatcatt
     1861 caggatgagt ttagccggct gcgctccggc gattttgttc gctttcgagg cagtttcttg
     1921 ggcgagaacc ggtttgaatc aaccggcatc ctcacacatc ccgactgacg aaggagaatt
     1981 atcatgcagt tttacatttc cttgcagccg ttgatcgcct tgattgccgg aattctcatc
     2041 ttgttgattc cgaggctgct gaattacgtg gtggccttat atttaataat taccggagtt
     2101 cttgggctga cgagaatgta gagaagaaaa gctgtgcaac cgtacaagaa gatcgagaca
     2161 aaatgcttgc tgattgtctt tttaaccgtg ggttaatgcg aaggaggata aggccatgct
     2221 aagttgggca attacttttt tgattatcgg cttaatcgca gggcttctag gacttaccgg
     2281 gatcgcggga gcggcgacac aaatcgcctg gatcctgttc gtagtgttcc tggttctgtt
     2341 tgtcgtcagc ttgataatgg gtcgtcgctc aagcgtttga ggtgaccata cctggaccgg
     2401 agtatgtttg cgctgtgccc gagcttggga ctgccgaggt gaactatcgt gaattctttt
     2461 cccatcctcg tcgccgagga caatcaggat cttctcaatc tgatcgtgaa gtacttggag
     2521 ctttgcggtt ggcgcgcagc ggcggcaaac acccgggaga atttttggct acagcttgag
     2581 cgacaaaggc ccgaattgat actacttgac gtgacgcttc acgtcagcga agcgacttcg
     2641 ttgcttaatg aacttaaaag cgatgggcgc tttaccgata ttccggtgat agccatgacg
     2701 gcgaacggct cgatgcgcca gaaaaatctt tgtatcgccg ccggatgtga tcgggtattg
     2761 gccaagccgt ttcacttgga tcaactgcgg cggcttgtcc gatgttttgc tagcaatcac
     2821 aagaacgaac aaaaaacagg agggatcata atgaagaagc cgatcaagca ctttgccgca
     2881 gtagcgctct tggctgtcgc acttttcggg tgtcaagcga tgaccgggcg aaccgcggga
     2941 caaaacgtcg acgatgcgcg gatctcgact gcggttcaga cgaccttgac gtccgacaag
     3001 gcatcgaatt ttacccgtat cgatgtcgat actacaaacg gcgtggttta cttgaacggc
     3061 acggtacaaa gcgccgaaca gaaggcgcgt gccgagcagt tagcgggtcg tgtcgacggt
     3121 gttaagaagg ttgttaataa cctgcaggtg ggcaaatctt agaatcgttg cgtcaaactg
     3181 gatctactca tctccaggtt cgggcggctt gctgatggcg gcaacgatgg acaacaatca
     3241 tgtgagacgg cgagcaagcc gagcaatccg atccgtgctc catcgggagt cagttaagcc
     3301 accgcccgcg cccaggcgag aaccgccaaa ccggcgcaag aagccgcccg tcaatgagcc
     3361 accttcgcgt aaacccggaa catcggatcc gcaagatccg cctcccgccg gcgatccacc
     3421 acccaaacga ccgccggaga agctaagcgc actgtttgtt cccgccacag tcgggttctg
     3481 ggctcgagcc gccagcagtc gaaagcatag acgggctgtt ccatgaagcc cgccgggcag
     3541 gaaggatcgg agcctcgcca ggatgaggac aaggccgatc tccaggactt gctgcccact
     3601 caacgtgctc tggatatccg ttcggtttcg ctcaccgggc ttttcgtact cgccaccttt
     3661 tacacactct attttgcgcg cgattttatt ctgccgatac tgctggcgtg gattttgagc
     3721 tctctcttgg cgcccattgt tcgcctcttt aaacgtgtgc gtatccctga acctttgagc
     3781 gcgctgttca tcatcttggc gttattgggg atattatctt acggcgttta ttctttatca
     3841 gatcccgttg cagcctggat tcatagagca ccgcaggtac ttaccgatgt ccgcgccaag
     3901 ctgcagaact tcattcaacc ggtcgcgcag gtgcagcaga ccaccaagga aattgacaaa
     3961 atgacgagcc tcggaaagga cgaggaagcg cccgcagtag aattaaaaaa acccgggatg
     4021 ggtgaagtcc ttttaaggcg cgctaaggat ttcttactcg gcggcggtgt gatgctgatc
     4081 ttgctttact ttttgctggc ttcgggagat ctgttcttgt tgaaactagt taaaatcctg
     4141 ccaacaatgg aaaacaagaa agtggctgtc gaaatttacc gacacatcga aaaagacgta
     4201 tccacctatc tgacagttgt aactctcatc tacatcggtt tcgggtgtgt cattggatct
     4261 gcgatgtatt ttctcggcat gcccaatcct tggttgtggg gcgtgatggc ggctgtcctt
     4321 gggtacatcc cttacctggg cgcgctagtc ggactcagta cggttactgt cgtggcgatc
     4381 ctcacatttg acgatatcta tagaatttta tcggttccgg tgatctactt taccgccgat
     4441 acgatccagg ccaatttgat cttccctatg gcgttaggcc ggagactggc tctcaacccc
     4501 gtagttattt ttatttggct gattttctgc ggttggatct ggggtatcac cggtgcttta
     4561 gtggcggtgc cccttctggc gattattaaa atcatctccg accaggtcga acaacttgcg
     4621 cccatcggcg aattcctcgg cagcgagagc aaggccgctt cgccctaacg tggcacgccc
     4681 aggcaatgtg aagaaggggt taataccgtt tgtgccagag aatgccaacg ccgcttgatc
     4741 ccgtggtgga cgtcgaagtg gtgatcttcc aatcgggtcc gatctggtat tctatcgaga
     4801 cttcctgtcc ggcttctccc gacaaatcct gtgagatgga aacataggtg cggtcgccta
     4861 tgtatcggcg gacgccgacc tgacctccgc cggcatcgat ctcaccgatg tccagtccta
     4921 atctgtccag tcccagcgcc tcagaaaccg cccctgcaac ttttgacgcc gcaaacccgc
     4981 ccgccatctc ggccgcactt tgctgcattg tgttctgttc gtttcggttc aaagtattaa
     5041 gcggcttgcc aaagagcagg agcgccaaaa tatcggcttg ttccaatgtc ggatcgctac
     5101 gtagcgtcag aacaggttta tccaaagtgc ccgtgacgat gacttcaact tggtattgct
     5161 gtaaacggtg ctgcgcgaca atgtcgagcg cgggattcag agtttcatcg cccctaaatt
     5221 ccaccgagcc gcggcttaga ttaaagcgtc tgccttgaaa accgatccat cctctcacga
     5281 ctgtaatcgt ccccaccaga gtcatctctt tgccagcttc tttaaccaca cgtagttttc
     5341 cgcttagctc cacgttggct tccgggtgtc ggatccacgc ctgattcggc acgaccactg
     5401 acaaatccac tctggcgttc ttaaaaagtt cattctcgcc tggtcgcgcg gttttgttct
     5461 cttggggctg cgcctcctcg agggttcgac ctcggacgat gacgatggtt tcgtcgcgcg
     5521 tcagcggtct cctaccacga cttagaaaag ctaagtctgg gcgcacttcg gcattgagga
     5581 cttcgatctg ccccttcaat gaaggctttc ccagaggccc ctgcacttcg atagtgcccg
     5641 ccaccgttgc ccggtatcgc tgtgtttcta tcaccggcca acgggaaagg gccagggaaa
     5701 tcttgaagtc ctcggggcta taatctttca gagataggat accgctgccg tttagacggc
     5761 cgtctttggc gcgggccgaa atctggcgca gcgaaacagt ttgtccgtcg aattttccgt
     5821 ccgcattgat ggcggcaacc tgcacgccaa gcggcttaag cgcgacgcct ccgtctcgca
     5881 gctgaaatgt gccgcggagt atcggtttgg tcaccgggcc ttgggcagtg acatctaagg
     5941 cgatgatacc agcgatactc tgcgccgcat cgccactgaa cgcgtttaaa aaagcgatgc
     6001 tcaaaccact tgattgaact cgcaggttca tatcgccggt agcttccgct tgccactggg
     6061 ggctccatct cagaataagc ggaatcgtgc cgtttgcggt caacacatga tcggcgttct
     6121 ggcgcacggt caacttgata tcggcggtgc gagatctgta ggcgatatca cctataatgc
     6181 cgccatagct ctgtccgccg atgctactat cggtcagatt cgctgatgcg ttgatttccg
     6241 ggtccgccgc cgttccggtg acctgagcac gcactgccaa agtcccggtc atagggagcg
     6301 gtttcggtaa caatgccgag actgtgctga tcgggaaccc ttccaccgtg accgcgagat
     6361 cctgtttacc gctggtggcg aatcggccgt tgatcgctac ctgttggacg cgatttctta
     6421 gcagcaaacc gtcgatgtcg aagttatcgc cgcgtatcat gatcgttgcc ggtcgggaga
     6481 gctgccaaac tccatcatcg aggctcaagc cgaccctatc cagtcgagcg accatcagtt
     6541 gcggctgata gtccaattgc cccgtgagaa tatgcgtgcg cttggcctga tccacggctt
     6601 tcaagtcgat cccgagctca taattctttt tagtggagag tccgatggag ccgtcgagtc
     6661 tttgcagccg gattcctgct tggatctcat cgagtcgaat cttcatggtt ccttgcggca
     6721 gggactgcgt accggcgagg cggaggtcaa aatcaatgtt gccgctcttg gttgtcgtat
     6781 tctgcagccg taatcccgca agcttcattg tgccttgtga ccggaggtcg gcaatattgc
     6841 cgctggcgcg gccggtcagc tcgatggaac ctgacccttt ctgattcgct aaaacgagcc
     6901 acggcgttag gttcgcggag cgaacaacat actgcagctt gccgttttgt tttggatcga
     6961 gaccaacatc gccttgcacc gtaagcattg catcctcagt cctggccgtt gcctcgagaa
     7021 tctgaagtcg ctgctgtgcg aggttggcaa tcacagaacc ttgttgcact tccaccggcc
     7081 cgatcgtcga tggcaagatc tttaggtccg tgctggcgac catgtcgctg ggagtgagtc
     7141 cggcgccttt gatagtgcct ttgaggctga tcttacctgt aatttgcttc tgatcgcccg
     7201 ctgctttttc tatatcgaga ttttccacgc caagagagag ttggtacgca ggtttttcct
     7261 tgagagaaac ttcgccttgc catgaagcga gacccatttt gccctttaag tttccattga
     7321 tctgcgcaac gctctcatgc attcggccat ccatggaaaa ttctcccagg gcccaagctc
     7381 caacaccggc attctggatc cgaatcacgc ctttggcatc gagatcagcc atcgaaagac
     7441 ccttgccgcc gccctcaaga gccgcgctcg caacgccgcc gatatcgttc ctgccaagca
     7501 atcttgctag atcaaaaccg cttaggttaa tctttccgcg ataacccggt gtttctccgg
     7561 ccgcgtcgag ctcaagattc cccgccaatt tagcgccggc cgccgccaaa tcgatatcaa
     7621 aagacagcgc attgagcggt ccgttgactg tgatagaccc gactacgtct tgcctgatcg
     7681 gccagtcggc aaaaacacgg ccaacatcgt gcggggcaag ccgttcaacg acgattttca
     7741 tgtccaagtt ggtgtcctgc aggtcggcga ttttgccatc gagtttgact cgcgagtctc
     7801 cggtggtaac tacgaaattt gccaattcaa gtctgtctgt accgtcagtt tcacggtacg
     7861 ccaaggcgcc atcgatcttc agctcaggta agcccggcga attgagttgc gcggccacgt
     7921 gactgaggtc gatagtgagc gtactagggc gagcatcgat ccggccgtcg acaatgacgt
     7981 tctctagcgt gtagttttgc ggttgcggac ccgtaagtct cagttcgatg tgtccttctc
     8041 gtaaactgac ggagttcaag gacaaaacga gccctgaggg ttcttcgctc tgtggctgct
     8101 ttggggaaaa agcttgcacg atgttcagct gattatcttc atcacgtctc aaacgaagcg
     8161 tcggccggct gcctgcgagt tgaaaaatgt gcaagcgtcc ccatatcagt ggaaatagtg
     8221 agtaggaaat cgtcagttcg tgcaccgcga gaatttcatc gccttcgtga cgtacgacga
     8281 catcgtgcag aataacattg ccccagagag agccgtcgat ttctcctaaa ctgagggagc
     8341 ccacgatcgt atcgttgatc gccgtcacca gtttatcgcg gacaaattca cgaaaccggt
     8401 cggtgtgagt gatgattgcc gccgcgatga tcaagagcag cagcaaaaac ccgagtctca
     8461 gggctatgcg atacaaaatg gccaacaatc tgctcattct aaaaggctgc ccctacactg
     8521 aagtgaatct gccaagcacg gtcgccgcgc gggggattga aaggaaaacc gatatctagc
     8581 cgcaatggac ccacaggcgt ggtatagctt aggccgaagc ccgttgagaa ttggagatcg
     8641 ttgacgggaa tatcaaatga tttcaacgaa acctggccga agtcgaggaa cagggcgccc
     8701 cctaattccc gccaaaccgg acgacgcact tccaccgaac cttcaatcaa gctgagaccg
     8761 ccgagcggtt cgtcgtcact gttgagaggg ccgaggcggc gcctgccgta accgcggaca
     8821 ctgctctggc cgccagcaaa gaagcgctca aataacggat agttcttttt tgagccgata
     8881 gcgtcggcga ctcctaactt taaacgtccg gcaaagatgg tgtcccagcc tatggggaag
     8941 tattttttcg cttcgcctgt tagtttgaag aattcatagg gaccacccca gatgccgccg
     9001 ccttgatccg cgttgaagga aaagatctca ccttggcttg gtttgaataa gtcatcagtg
     9061 ctgttccaaa ccagccccaa agaaggaccg gtcaagatac ctttcgttct gatctctccc
     9121 aaagcttgtg gcgtcgcgtc cgccacatca ctaagctgat tgtactcgac gcgatagccc
     9181 aagaagctaa gaagccttgg cgagaagcga tgctcaagac ggccggaaaa tcgacttacg
     9241 ttgcgtttga aggtttcctc gtcttcctga tcgtggcgca gatttagtcc ggcctgggtc
     9301 tgccgtgtaa aaaagtgagg ctgaataaaa tcgattgctc cactgacgag gatggaagag
     9361 tatttgcctc gaatcgacaa tcgccttccg tcgcccagcc agttttgatg acgccaatcc
     9421 agtgaaacgc ggaattcctc ctctgtgctg taaccgaatc cgagactgat ctcgcgtggc
     9481 ggcttttcag taacttgaac ttccatcgga accaccgttg acttttcggc actctcccgg
     9541 ggattgattg cgatggcgct gaacaaatcc aacgccagga tttgctggcg tgattcccgg
     9601 atctttttta gcgagaacat ttcgccgggc tggtaagcca gctctctccg gatcaaatag
     9661 ggttcgacct gctcgcttcc cttgatctcg gtttcatcaa acacagcctt gggtccgggc
     9721 tgcgccgagt agcggacaac agcctgatct tgttccagat ctacctcggc gcggcgttgt
     9781 gtctgagtgt gcgcatggcc gttgtccatc agatgattac gcaagaagag ctccgcgtcc
     9841 tgatacttct gttcccggaa aatctcgccg ggggtgaagg gaaatttctc gggcaaaggc
     9901 ggatgctctg gcgattcagc gttaacttct ataagcacct gtgagaccac gaccggactg
     9961 ttctcttcga tgtaaatgtt gagactcacg aggccgcgag tgtcatcagg atcgatgtcg
    10021 tgagttatga ggtttttgta ataaccctcc gactgataaa agcgcctcag ccgatcgagg
    10081 tccgtcgcaa acgtaaccgt gtcgaaaagc ggccgctctt cccacaaccc gtaccagggg
    10141 cgtgcctggg tcaccatttc ggcgagaagt tggctatcgg aaaaacgctg gtttcctgaa
    10201 atctttattt tgtccacccg ccactcgcgc gccgaatcga gctgttcgag ttgaatggcc
    10261 atgacgcggc acatcaacag tagccacacc gccagtgcag agaacaaaca gaatatgaat
    10321 ttcatttacc gatctcgaat atccagttct caactaacat cctcatcaac gcaagcgtga
    10381 attgttcgtt cccgtgcctt tgcttctcca ggcttatcgc tccatcggta tacaagcgtc
    10441 catcagcttg gagattcggg ttcatttagg tttgcaccct tcgtccgccg gctcaatagg
    10501 ctcctaagtc ggagcaccat cggcaccctg acagaattgg atctacccgt gcctttctcg
    10561 ttcgaggctt cgcttgatca ttgcagtgtg tctggatgat tcggagctgg aaactgggcg
    10621 gctctgaaag cgcgacgatg cggcaacggt gctgagaacg tggtcgtgta ttcttacttc
    10681 tattcgagtt gatgtagcaa tggctaatcg gaatcggtgg gcatggctta ctctcggtag
    10741 gcgatcgtgc tgagtaacgc gttctctgcc tgacgcgagt caaggcgtgt atgctggacc
    10801 acgatcggta tgtcggattc gatgacgctg gcaaaatcgg tagccagggg aattttttcc
    10861 ggatctctca aatcgttaaa ccggacgtgc tttgtccggc gcgcggccac cgtaatcgcg
    10921 tacggcccgg caggttcgcg atcagagaag aagacggtaa tcacgacgtg agcgtcttga
    10981 tccgcggtgt tgagcaagca gaccgtctca tggctggtga attcgggttc gggaccgttg
    11041 ctccagccgg gaatatagcc ctccgggatc gcccaaagcc ttgctccgat tgcgttcatc
    11101 tgtgtctcct agtttggttg cccgggccgt gagccggctt taagtttccg ccggtatctg
    11161 cgctctggcg tagcgaaccc tttgttcggc tcgacgattt cgaacgggaa ttccctcgcg
    11221 aggccgtcga cgctataacg caaccaacat gccaatagca tttagcgagt gcgtttcagg
    11281 aaacgcgaat gctgtccagc tgtggaattc accgcagatt acgttccggg tgtgagacgt
    11341 cctaggagtg tgctaagctt tgatattgat ttatcgccga gattagttgc atgataagca
    11401 gagaccgtag tcggctggct atgcggatta aaggtcacgg tagagataag gcgcgatgac
    11461 agtcaacgaa cccgcaattc aaagccgagc atgtgcgcca ccgctcgacg agagcatcct
    11521 ggtagcgctc tacaacggaa ttcctgaagc ggtaattgtt atcgaattcg gttcgcgccg
    11581 tatcgtctac tggaacgacg gcgcgtcctt gttgtttggc tattcggctc aagagatctt
    11641 gcagcagacc accgaacgac tctatctcga caaaaactgg ttcgatcgta tctaccaaac
    11701 ctccactcct gaaatcgagc aaagcggctg ttggcgcggt gaatggcaat atcgccggcg
    11761 cgacggatcg cttttcgccg cggaggcgac ctgcacgctt ctgcgaacgg ataaaggcgc
    11821 ctatgtggtc aaggttattc gtgaggttac cgagcgcaag gaagcggaga agattattct
    11881 cgaattgaat cagcgcttgg cgcagcgcgt cgtcgaacaa acatccgagt taatgtcaaa
    11941 aaccgaggag ctccagcatg aggaggcgca acgtaaacag gttgagcaaa tcctcggcaa
    12001 tttgctccgc aacatcacca attacgcggt tctgataacg gatgccgatg gccgcgtcgt
    12061 tgactggaac cgcaccgcaa cgcgcatcct gggccgcgag agtgccgaca tgacagacca
    12121 gcggctgtcg gttttctttg ccgatgaaga aggacggcag gcgttgatac aaaaggcgct
    12181 cgaggaggcg agtgatcaag gaacgtttcg cgattaccaa ctgctggtgc ggaaagacgg
    12241 ctcacgattt tatgccaggg tcattgtctt tgcgctgcgt gacaatgccg acggcttgca
    12301 gggatttctg attctcctgc gcgatgactc acaggagcgt aacctgagag agaagttacg
    12361 cgaaaaggaa catctggcgg cgattggaac cgcggcatcc atgctggctc atgaaatcgg
    12421 taatccgctg aacggcattt ccaccaccgt gcaactgctt gagcggttta tcagccgaga
    12481 ggggatcaaa gcgccggcgc acatgctcgc gacgttgaaa gacttgaaaa gcgagatcga
    12541 ccggcttact tcgctattga atgaattcaa aactatcgcg cgccctcaaa gacttaacgt
    12601 tcaaccggtg aacctcgcca gcgtcattca agaaacgatt tcaggattgg atatggacgg
    12661 caaaggggac gtgcagattg tttgggactg tgcaccggac ctgccgccca tccgcggtga
    12721 cgcggccaag ctcaagcagg catttctcaa tctttttaaa aatgccgtgg aggccatgcc
    12781 atacggcggc actctctcta caaagggata cctaagcgcc ggctcgatct gtatcgacat
    12841 cagtgacacc gggatcggta tccccgatga tctaaaggtc tttgatctgt tcagctcgac
    12901 aaagcctcac ggtaccggtt tggggttgtt cattgtgcag caaattattc ttgcgcacga
    12961 cggcgtggtt tcttacgcca gcaaagtagg caaggggacg acctttcact tgaccttacc
    13021 tggaagcaca acaccggaag gggttgccga cacccgccgt aactagcttc gggggtatca
    13081 ccacaaaagt gatccccctt acagccatct cgctgttact cggagcccaa ggcgcggtcg
    13141 agttcatcgt ctttgaatgg cagccgctcg gtatctccgt tggcccctaa tgtacgttgt
    13201 ctcttctcca atgcggttgc gcaatcgatg caaagcgccg ctgtcggcat tgccttgagc
    13261 cgaccagggc ctatatcatt gccgcatcgt tcacactcgc caaaagttcc cgcatcgatt
    13321 cgctccaaag cctcatcgat ttgccgcagc aatatctgcc cgcgctctgt gaggctactt
    13381 tccaaacgag tcatgcgatc cttttgagcg ctctcctcga gttcgctttc acgatcttct
    13441 agcgccggct caacttcatt ctgcgtaccg acgatttcct gcaacaacag cgaagagcgt
    13501 ttgcgccgca gagtcgcgcc gagttcctta gccaatcgat tgttcatttg aagttctccc
    13561 ttagtcatcg taacgaaaga atcgccaccg gcatgccgac atgagtgcga tatcgtgaca
    13621 atcatcctgc gcatttgttt tacctcagat cacgctagcc agcgcggccg agtcgcccgc
    13681 gctgactctg gccgaggggc gctgaggcat aatctagcac cgctttaaag cgtcaggttg
    13741 gtgtgcaaag cgaaggccaa gtgtagtaac cggactgatc tcggttgagt gaagaagcgc
    13801 cgagtggatt ggttcgcgat gggctatcac tttttccggc gtcctatagt tggcaatcta
    13861 atcgaggcag tcgtcccgtc cctgggaaga gagtctctaa gcctacaatt gtctgtctgc
    13921 agttctcaat ttactgccgc tcaccgct
//
LOCUS       DASUVS010000018         3569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_223906, whole genome shotgun sequence.
ACCESSION   DASUVS010000018 DASUVS010000000
VERSION     DASUVS010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223906"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            317..640
                     /locus_tag="VF182_01020"
     CDS             317..640
                     /locus_tag="VF182_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435679.1"
                     /translation="MKRFLTLAIAFLFMATVAIAAEPKDYQVTGPVLDVKEDVIVVQK
                     GDEKWEIGRDKGTKIKGGDLKKGSRVTVHYKMTASSIDVKDGGDAKSGTKTKSDDKKA
                     ASKTK"
     gene            899..1804
                     /locus_tag="VF182_01025"
     CDS             899..1804
                     /locus_tag="VF182_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435680.1"
                     /translation="MNVIYPAISGINAPLWYATEQNIFPKYGLDVELIYIPTALQVTR
                     VVLTGDSQIAFSGGAPVVNAVLSGADLVFIGGVANVPAFYMMALPEIKSVADLKGKTV
                     GVTRFGSSTDFALRYLVQKNGFNPERDLNILQLGGMPELAAALSKRLIVAAPLSAPTH
                     IRARAAGAQPLLDMAKAGVYFPHTAVITRRAYLKTNRDTALNFFRGYSEGLQRFVQDK
                     NAAKKIIQKYTRDTAEDIIEATYQYAVDYVVRPPYPNREGIVETLKLSQNPEAKKANP
                     DNFLDSSIVKTLEDEGFLRQIGMQK"
     gene            complement(1944..3044)
                     /locus_tag="VF182_01030"
     CDS             complement(1944..3044)
                     /locus_tag="VF182_01030"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6435681.1"
                     /translation="MMILNNEEVGALLSMKNCLRHLEAAYRELAEGSAVNRPRSDLYL
                     PSTGSGGVYCFKTMEGALSRERVVALRLNSDVIKWEEKGGRIIKDKVPAAPGNKWVGL
                     VLLFSAETGEPLAIFPDGIIQGIRVAASSALAARFLARPEATVLGMLGSGWQARAHAQ
                     AMCEVRDIEQILVHSPTKANRESFAAEIEKQLGVPVHPVTSGEAVADRADILVAATNS
                     VSRVVPPDWLKPGMHLTCVKITELGDETLRKADRLVIHTRKWAPDNYITGFNDEKVEA
                     HDPIDLLIESAKRTSFTPKQPFWLNAPELKDVVAGKLPGRQSPEEITCFNNNIGLGIQ
                     FAALGKAVFDDATAKGLGREIPTDWFLETVHP"
BASE COUNT          824 a          990 c          899 g          856 t
ORIGIN      
        1 atcgagcttt ggattacacg ttaacagtgg cggggttctt ttcagtacct aactccccag
       61 tctttttagg cccgaacatg ttcgtcctgt tgcccgcatc aatattactt ttcatccgtc
      121 caatattaga gatttgtttc tcacaagttc attattccac ctcttaccgg acttagctat
      181 agagtgatct cggcaacgag catggtcctc actcagcggt tgaaggcgca cgataagcgt
      241 tgtcacgttg actgcaatat gggatagagt atagccatcg caaagctcac taaactcaaa
      301 caaggaggat ttgacgatga agcgttttct aactttagcg attgcatttt tatttatggc
      361 gacggtagct atcgccgctg aaccaaaaga ttaccaggtt accggaccgg ttctcgacgt
      421 gaaggaggac gtcattgtcg ttcagaaggg tgacgaaaaa tgggagattg gtcgcgacaa
      481 aggcaccaag atcaaagggg gcgacctcaa gaaaggttca agagtaaccg tccattacaa
      541 aatgaccgcg tcgagcatcg atgtcaaaga cggcggcgat gctaagagcg gcaccaagac
      601 caagagtgac gataagaagg cggccagcaa aaccaagtag cttgatgcga taattttgag
      661 gtgttttctt cataggatct ccgaaccggg ccggcaacgg cccggtttac tttcactttc
      721 cccgcttacc atgcatttac cgtgtaacat gcctttgtct caccactacc atgcttgaat
      781 tttgaataat gattgggggt ttcacatggt ctttcgagcg atgaagtcct ctatcctagc
      841 cgccacactg ctcatgtgcc tgacagcaag ctcggcttgg ggccagacac gaacgaaatt
      901 gaacgttatc tacccagcga tcagcgggat taacgctccg ctctggtatg ccaccgagca
      961 aaatattttt ccaaagtacg gcctcgacgt tgaactgatc tacatcccga ccgcgcttca
     1021 agttacccga gtcgttctga ccggcgactc ccaaatagcc ttttcaggag gcgcgccagt
     1081 ggtcaacgca gtgcttagcg gcgccgacct ggtcttcatc ggcggcgtgg ccaacgttcc
     1141 ggccttctac atgatggctc ttcctgaaat taaaagcgtc gcggacctga agggaaaaac
     1201 ggtcggcgtt acccggttcg gttcgtcaac ggatttcgcc ctacgctatc tcgtgcaaaa
     1261 gaatggcttc aatccggaaa gagatctcaa catacttcaa ctaggcggca tgcccgaact
     1321 tgcggcggcg ctttcgaaac ggctcatcgt cgcagcgccg ttgtccgcgc ccactcacat
     1381 ccgtgcccgc gccgccggcg cccagcccct gctcgatatg gccaaggccg gagtctactt
     1441 tccccacacc gcggtcatca ccaggcgggc ttatctgaaa accaatcgcg acacggcgct
     1501 gaattttttt cgtggttatt ccgaaggtct gcaacgattc gttcaggaca aaaacgccgc
     1561 aaaaaagatc atccagaaat acactcgcga taccgccgaa gatattatcg aagcgactta
     1621 tcaatacgcc gtcgattatg tagtgcgccc gccctatccc aatcgcgagg gcattgtcga
     1681 aactctcaag ctaagccaga atcccgaagc aaaaaaagcc aacccggata attttctcga
     1741 cagcagcatc gtcaagacac ttgaagacga aggatttctg cggcaaatcg gcatgcagaa
     1801 gtaatcttgg cttccaccac gaagtgtgtt gctgagtcga ttatcccgga acgaaactct
     1861 gcctactcgg tgcccctgcg gtaactatct cttaacttcg tcctcttagc gaccttcgtg
     1921 gtgaatccta gctttgtctc gttttaggga tgaaccgtct ccaaaaacca atcggttgga
     1981 atctctctgc ctaatccttt agctgtggca tcgtcgaaaa ccgccttgcc cagcgccgcg
     2041 aactgaattc cgagcccgat attgttattg aaacaggtga tctcttcagg cgattggcgg
     2101 cccggcagct tgccggcgac cacatccttc aactccggcg cgtttagcca gaacggttgc
     2161 tttggagtga agcttgttcg tttcgcgctc tcgatcaaaa gatcgatcgg gtcatgcgct
     2221 tcgactttct cgtcgttaaa accggtgatg taattatccg gcgcccattt tcgcgtgtga
     2281 atgacaaggc gatcggcctt gcgtagggtc tcgtcgccaa gctcggtgat tttcacacag
     2341 gtgagatgca tgcccggttt tagccaatcg ggcggaacca ctctcgaaac cgaattggtc
     2401 gccgccacca gaatgtcggc gcgatcggca acggcttcgc cggatgtaac cgggtggact
     2461 ggaacgccga gttgtttttc gatttcggcg gcgaagctct ctctgttagc tttcgtcggg
     2521 ctgtgaacca gtatttgctc gatgtcacga acttcacaca tggcttgcgc atgagcgcgc
     2581 gcctgccacc cggatccaag catgccgaga accgtggcct ctggccgagc taaaaagcgc
     2641 gccgcaagag cgctgctcgc cgcaactcga ataccttgaa taatgccatc gggaaaaatc
     2701 gccaggggct ctccggtctc ggctgaaaat agcaacacca gtccgaccca tttattccct
     2761 ggcgcagccg gcactttgtc tttgatgatg cgcccgcctt tttcctccca tttgataacg
     2821 tccgagttaa ggcgcaaagc caccaccctc tcccgactga gcgcaccctc catggttttg
     2881 aaacaataaa ctccgccgct gccggtgctt ggcagataaa gatcgctgcg cggtcgattg
     2941 acagcagatc cttccgccag ctcccgatac gctgcttcca ggtgccgcag gcaatttttc
     3001 atcgacaata gcgcgcccac ttcttcgtta ttcaagatca tcatggctgt atcactatct
     3061 ccttccgttg cagttcttat cgcgaagccg gagctcgcag tcaagcggat tcagcggcgg
     3121 attccacggt acaattggca ggaatgacca gagcgctctt gaagcttcac gttcccaggt
     3181 tgcgggtgct tgtcacacaa ctatagggcg gaggagattt cttgttgact aatctagttg
     3241 gccggacaac tcgaattttc caccagacaa tcgagaagtg aaatgagatt gcgttgatga
     3301 gaatttcatc catccgatgg aaatctttaa tactgttgga tcggcgctag cctatggcta
     3361 tcgggcaccc gccgggtctg cctaggtggc atcgatatcc gggcgcgcct gttagcgcgt
     3421 ttgtcgaaaa tacgggtcct gcgatcgcgg tgcaaaacac cgtggcgccg cggagcagtg
     3481 attggcgcca atgatgtttg cagtgcggcg aaaggacggc ttggtcttgc tccatttccg
     3541 tccagcaccg tattggacag ttttctgac
//
LOCUS       DASUVS010000019         4602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_878920, whole genome shotgun sequence.
ACCESSION   DASUVS010000019 DASUVS010000000
VERSION     DASUVS010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878920"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VF182_01035"
     CDS             <1..347
                     /locus_tag="VF182_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEX6435682.1"
                     /translation="EKPQLARMLRGKVSLQRKYNLTSIPIRSTKDILRAEEEFFIKVW
                     YGRKQSAAAYRAQGTPEYIIKGMLKAKRDAEKLYGKKNLGPWDDFEWGMLSGKLSALR
                     WVLGDEWDMLDT"
     gene            complement(439..1566)
                     /locus_tag="VF182_01040"
     CDS             complement(439..1566)
                     /locus_tag="VF182_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435683.1"
                     /translation="MTAPKPVTVWAVAVLRPALSLVLNNKRCAAFLMHIMAAVAFSKS
                     CMIIFLPLLWSVVLSHGSLFAAELEKVRVGLSSISATHGALWVAEEKGLFKKYGLDPE
                     VIVIGGGGARGVSALIAGDIQFVTAAGDSVISAALRGADAVMIAGILNKGVQRVMTRS
                     EIKTPADLIGKRVGVTRYGAASHLVLLMMLKKWGISSDKVRVLQVESSSAMVVNLDKG
                     GIDAAVLTIPSNFVAEDKGYRVLADLGDMDIYYLNMMLDTTRAFLRSRRDTVVKFTKA
                     FVEGIAYFKKNKNESIAVLKKKLRSDRGGDDFLARSYDVYASNQYEQVPYPSIEGVKT
                     VLEFLAQENPKARGADPNSFIDNSIIKELEASGFIRDLYMK"
     gene            complement(1707..2861)
                     /locus_tag="VF182_01045"
     CDS             complement(1707..2861)
                     /locus_tag="VF182_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6435684.1"
                     /translation="MDFDLPEELQMLKDTVRKFVDKELIPIEMHTIENMELKPDIRER
                     LEKKTRDMGLWMFDVPEEYGGGGLGSLAQVLVWEELSRTVALPSRGQGIFGPEVRPIL
                     YALNPDQKKRFMDPVLRGEKKLCFMQTEPDAGSDPASMKTRAVRQGDHYIINGTKRFI
                     TGAGDADIGQLMAVTDPAKGAHGGISCFMVDMKTPGLTLVTRYKTMMGEEPWHITFDD
                     MKVPAENLVGKEGEGFKLAQKWLGIGRLKHGARALGVAERCIEMGASYSKQRITFGKP
                     LSERQGITFKLADSYVELHAARLMVYQAAWKNDQKKDIRNEAYMAKLFADEMSFRVVD
                     RVLQIHGGIGLTLDLPLAKWFVDQRSRMITEGASEVMRMVIAREVLKKYN"
     gene            complement(3003..3821)
                     /locus_tag="VF182_01050"
     CDS             complement(3003..3821)
                     /locus_tag="VF182_01050"
                     /EC_number="4.1.2.61"
                     /inference="COORDINATES: protein motif:HMM:NF006588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxycinnamoyl CoA hydratase/lyase"
                     /protein_id="HEX6435685.1"
                     /translation="MAEYQTLLVDKNDGVTTITLNRPEKRNAMSPQLHRDMYDLLTDL
                     RYDKDTRVIVITGAGESFCAGQDLKQYFIEMDSQPARVRDEVREKSRQWRNEILRKMP
                     QPVIAKINGWVFGGGFTVVTGCDIAIAADDVQLGLSEVNFGHFPGGEVTIVLTEHLQP
                     KHGLYYALTGKTMTAKEAERIGLITKAVPRADLDKEVTELANNLKEKAPSALNAVKEA
                     WYYTFYSNPDVAYEISNLISDKAKQAIGGRPGLQQFARKEYRPGLGSYKFEEKK"
     gene            complement(4190..>4602)
                     /locus_tag="VF182_01055"
     CDS             complement(4190..>4602)
                     /locus_tag="VF182_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX6435686.1"
                     /translation="QLRLLEMKMHMISGGAIHELMAAGEMPLSVSIFRNHVLAGQAKG
                     APTEWVPLDLNATNAGGVALPSNSNNPYAALLLADFLLSPEGQKIFEEKFRFATSTKD
                     YGFKRWYPEKGLTTEQYEKFDDRWKKLLRDITRK"
BASE COUNT         1046 a         1275 c         1199 g         1082 t
ORIGIN      
        1 gggagaaacc acagctcgct cggatgcttc ggggcaaggt aagcctgcaa agaaagtaca
       61 atctgacgag cattccaatt agaagcacta aagatattct gcgtgcagag gaagagttct
      121 ttatcaaagt ttggtacgga aggaagcaga gtgctgcagc ttatagagcg caaggaacgc
      181 cggaatacat catcaaaggt atgctcaaag ccaagcggga cgccgaaaag ctctatggca
      241 agaaaaatct cggtccatgg gacgatttcg agtggggaat gcttagcgga aagctctcag
      301 cccttcgttg ggtgcttgga gatgaatggg acatgcttga tacatgagag tcatccgtta
      361 cggtgcttgg tggaactgac taggcacagg ccgaagcaag agcaaagctg tctaaatttt
      421 gaaagcgtct gtggagcttc atttcatgta aagatcgcgg atgaatccgc tggcttcgag
      481 ttctttgatg atcgaattat cgatgaacga gttgggatcg gcgccgcggg ctttgggatt
      541 ttcctgggca agaaactcca gcactgtttt gactccttcg atcgaaggat acggcacttg
      601 ttcatattgg ttcgatgcat agacatcgta cgaccgagcg agaaaatcat cgccgccccg
      661 gtcgctgcgc aacttctttt tcaataccgc aatgctttca ttcttgttct tcttgaaata
      721 ggcgatgcct tcgacaaagg ctttggtgaa tttcacgacc gtgtcgcgac ggctgcgtag
      781 aaaggctcgg gtcgtgtcca gcatcatgtt gaggtaatag atatccatgt cgcccaaatc
      841 cgcgagcact cgataacctt tatcttcggc gacaaagttt gaaggtatgg tcagcaccgc
      901 cgcgtcgata ccgcctttgt ccagattgac caccatggcc gatgacgact cgacttggag
      961 cacccgaacc ttgtcggagc tgattcccca tttcttaagc atcatcagca ataccaggtg
     1021 cgatgctgcg ccgtagcgcg taaccccgac tctttttcca atcaggtctg ccggcgtctt
     1081 aatctccgac cgggtcatta ccctctgcac gcccttattg aggatgccgg cgatcatcac
     1141 cgcatcggcg ccgcgcagcg cggcgctaat caccgagtcg cccgctgcgg tgacaaactg
     1201 tatatcgccg gctatcaacg cgctcacgcc tcgggcaccg ccgccgccaa taacgattac
     1261 ctcgggatcg agaccgtact tcttaaacaa gcccttttcc tcggcgaccc agagggcgcc
     1321 atgagtggcg ctaattgaac tcaagccgac gcgcactttt tccaactcgg cggcgaagag
     1381 tgagccatgg ctcagaacca ccgaccacag caaaggaagg aaaatgatca tacaggactt
     1441 ggagaacgcg actgcggcca tgatgtgcat caagaaagca gcacagcgct tgttattgag
     1501 aactaacgat agcgcagggc gcaacacagc tacggcccaa acagtcaccg gtttaggagc
     1561 cgtcaccccc gaatgttttc atcgggggtc cagttccgga gcgcctggat tcccggcaaa
     1621 accgtccgga atgacggaca tcgaacaaga gaatatcaat aggccaaagt gtgagaaatg
     1681 catggagcag cttaaacgtt gagggattaa ttgtatttct ttaaaacctc gcgcgcgatg
     1741 accatgcgca tgacttcgga agccccttcc gtaatcatgc ggctgcgctg atcgacgaac
     1801 catttcgcca gcggcaaatc caaggtaagc cctatgccgc catggatctg caagacccgg
     1861 tcgacgacac gaaacgacat ctcgtcggcg aagagttttg ccatgtaggc ttcgttgcgg
     1921 atgtctttct tctggtcgtt cttccaggca gcctgataaa ccatcaagcg cgccgcgtgc
     1981 agctcgacgt aggaatcggc cagcttgaat gtgatgcctt gccgttcgga caggggcttg
     2041 ccgaatgtga ttcgttgctt ggaatagctc gcgcccatct cgatacagcg ttcggcgact
     2101 cccaatgcgc gcgcgccgtg ctttaagcgg ccgatcccta gccatttttg cgccagcttg
     2161 aagccctcgc cttctttgcc gacaagattc tcggccggga ctttcatatc gtcgaacgtg
     2221 atatgccagg gttcctcgcc catcatggtt ttgtatcggg taacgagcgt tagacccggc
     2281 gtcttcatat ccaccatgaa gcaggaaatg ccgccatgcg cgcctttggc cggatcggtg
     2341 acggccatca attggccgat gtcggcatcg cccgcgccgg tgatgaagcg cttggtgccg
     2401 ttgatgatgt agtgatcgcc ctggcgcacg gcgcgggttt tcatcgaagc aggatcagag
     2461 ccggcgtccg gttcggtctg catgaagcag agtttcttct cgccgcgcag aaccggatcc
     2521 atgaaacgtt ttttctgatc cggattgagc gcatagagga tcggacgcac ttcgggcccg
     2581 aagataccct ggccccgcga cggcagcgcc accgtgcgcg acagctcttc ccagactaat
     2641 acctgcgcta aactgccgag cccgccgccg ccgtactcct ccggcacatc gaacatccac
     2701 agacccatat ctctggtttt tttctccaag cgctcgcgaa tgtccggttt gagctccata
     2761 ttctcgatcg tatgcatctc gatcggaatc agttccttgt cgacgaactt gcgcaccgta
     2821 tctttcaaca tctgaagttc ttcaggtaaa tcaaaatcca tgcatgtcct ccggcacaac
     2881 gctgctacgt tcaatcgctg cgctcttcct acttctcctt ttttaatcct cccccgcacc
     2941 gcgggggagg aaagaagtgg gggctgcaac cattggaact gcttgaacgg tcggaactcc
     3001 cgttattttt tctcttcgaa tttataagac ccgagccccg gtctgtattc cttgcgcgca
     3061 aactgctgca atccaggccg gccaccgatg gcttgcttgg ctttatcgga aatcaggttg
     3121 gaaatttcgt aggcgacgtc gggattggag tagaaggtgt agtaccaggc ctctttgacg
     3181 gcgttgagcg ccgacggcgc cttttctttg agattgtttg cgagctccgt cacctctttg
     3241 tctagatcgg cgcggggcac cgccttggtg atgagaccga tgcgctcggc ttctttggca
     3301 gtcatagttt ttccagtcag cgcataataa aggccgtgct tgggctgtaa atgctcggtc
     3361 aatacgatgg tcacctcgcc gcccgggaaa tggccgaaat tgacctcgga aagcccgagc
     3421 tgtacgtcgt cagccgcgat cgcgatgtca cacccggtga caacggtaaa gcctccgccg
     3481 aatacccagc cgttgatctt cgcgatcacc ggctgcggca tcttacgaag aatctcgttg
     3541 cgccattgac gcgacttttc ccgtacctcg tcgcgcaccc gcgccggctg ggagtccatt
     3601 tcaatgaagt attgtttgag atcttgcccc gcgcaaaaac tctcgccggc tccggtgatg
     3661 acaatcactc gggtgtcttt gtcgtaacgc aggtccgtaa gcaaatcgta catgtcgcga
     3721 tgcaactgcg ggctcatcgc attgcgcttc tccggacggt tcaaggtaat cgtcgttact
     3781 ccgtcgttct tatcgactag caaagtctgg tattccgcca tcatcatctc ctttagctat
     3841 ttatgatctg gattggttct caattgttac taggacaaac tgtctttgtg agctctatcc
     3901 acttaacaca agcctgtcaa atgagcgcaa gcattgctga ccgactaaaa taaactgcct
     3961 tgcagcgagc tttgaggcat cagaagagag caataaaaat tttcccaaaa catcaccaaa
     4021 catgcgggaa tgacggagta acgattcacc attacgcggc aagcggcagg gaattaaccc
     4081 ggagcgatta aagttcgact caattgcgac gtcgtgcgcg gatgtcgtga gaggttaaac
     4141 gatcatcgaa tctgtcggat tagaatgtcg cgccgcaaac gaccaaaatt cacttccgag
     4201 tgatatcgcg cagcagcttc ttccagcgat cgtcgaactt ctcgtactgt tcggtcgtga
     4261 ggcccttctc gggataccat cgcttgaagc cgtagtcttt cgtcgatgtc gcaaagcgaa
     4321 atttctcttc gaagatcttt tgcccttccg gactgagcag aaaatcggcc agcaacaatg
     4381 ccgcatacgg attgttgctg ttgctcggca aggcaacgcc gccggcattg gtcgcattta
     4441 aatccagagg aacccattca gtcggcgcgc ccttggcttg tccggccaaa acgtgattac
     4501 gaaatatcga gaccgacaat ggcatttccc ccgctgccat caattcatgg atcgcgccgc
     4561 ccgagatcat gtgcatcttc atttccaaaa gccggagctg ct
//
LOCUS       DASUVS010000020        17557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_5667, whole genome shotgun sequence.
ACCESSION   DASUVS010000020 DASUVS010000000
VERSION     DASUVS010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 17557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17557
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5667"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VF182_01060"
     CDS             <1..663
                     /locus_tag="VF182_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006921955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEX6435687.1"
                     /translation="GRGWRVHRMTDPGVYRALTALLLLSPQTPMLFQGQEFAASTPFA
                     YFADHEGKLAEKVKEGRREFLHQFPSLAAEAMQREIPDPTDPRVFASCKLDFSERSRH
                     HEIYALHQDLLRLRRADPAFNSQEPGALDGAVLGARAWVLRFFAPEGKDRLLLINLGI
                     DIMLGPVPEPLLAPPFGAIWQVLWSSENPRYGGAGALPLENENGWLVIGKSAVVLADS
                     PK"
     gene            660..2684
                     /locus_tag="VF182_01065"
     CDS             660..2684
                     /locus_tag="VF182_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX6435688.1"
                     /translation="MTPPLVRTLTDVPQIKLEPPELTHHPEWLVTNGLGGYASGTVSG
                     AMTRRYHGLLIAAFPAPLGRMLLLSDLNEEVHFPNGRFVQLSTRYGDSEPTARLIEFR
                     LEMGLPVWRYQIDDWMLEKRLYLPYRQNTVFITYKPLACPDHIRLKLRPLIQYRGHSD
                     DVDAQPADPYVLTVLEDQYQISTGNLPPLRLLPYGKGEFVIDRKKIEKVRYLREEQRG
                     YHVIGELWSPGTFVLQAAAGEESTLVASAQSWEIMRAMRPGEALAAERDRRTRLITSA
                     DNRAHSGFAAELVLAADQFITVPAGRLADQTRTHAAGDEMRTVIAGYHWFTDWGRDTM
                     ISLEGLTLVTRRFLEAGWILRNFAKSIRHGLIPNLFPEGSREGLYHTADATLWFFHAI
                     ERYVQATGDRWTLRFLLPKLIEIFEAHIRGTLFGISVDPQDGLLRQGEEGYQLTWMDA
                     KVGDWVVTPRRGKAVEIDALWYNALRLLQKWVEEEEDRKLAREIGAWADKARNSFNKK
                     FWCADKGYLYDVIDGPHGNDAACRPNQLLSISLDHPILESDRWKPVLRTVQERLLTPF
                     GLRSLAATHADYKTRYFGDIRARDAAYHQGTVWAWLIGPFIDAWLKVYPEDRSGAHEF
                     LRGFIPQMNEHGIGTISEVFDAEQPWTPHGCIAQAWSVAEVLRCWIKTAP"
     gene            2955..3386
                     /locus_tag="VF182_01070"
     CDS             2955..3386
                     /locus_tag="VF182_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6435689.1"
                     /translation="MQIRDIMTRDVEVVSIDAPVREAAAKMKQLDVGAIPVCDGQKLT
                     GVLTDRDIAVRLAAEGRDPSETRVSEIMTRDLYYCFEDQDVEEAATVMEAGQIRRLPI
                     LDHDRQLVGIVSLGDISVRSDEKEAAGEALEGISEPAAPKR"
     gene            3640..4530
                     /locus_tag="VF182_01075"
     CDS             3640..4530
                     /locus_tag="VF182_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX6435690.1"
                     /translation="MARSSKAAPKKKTRSASKPAGRALWKGSINFGLVNIPVALYPAE
                     TSQSLDLDMLDRRDFSPIKYQRINKKTGKEVPWDQIVKGFEYEKGEYVTLTDEELRRA
                     NVEATQSVDILDFVGAAEISPVYFDKPYYLEPQKNGRRAYMLLREVLTKSNKVAIARV
                     IIRTREHLAAVIPQGPVLILNLLRFQHELRDSKGLDIPETGRKGRAISANELRMAERL
                     IDAMSAPWNPAKYRDEYREDVENLIEKKIKSGRPRTVSGGGKASARKTGGKVVDIMSL
                     LQRSVVQAQKKDNAARRKAS"
     gene            4783..6795
                     /gene="ligD"
                     /locus_tag="VF182_01080"
     CDS             4783..6795
                     /gene="ligD"
                     /locus_tag="VF182_01080"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX6435691.1"
                     /translation="MKRNNPNTTARADSEQEADLRGTRRKAIKKSPPSPVARARKSRL
                     PDFVAPQLATLVDRVPQGDEWLHEIKFDGYRILCRISNDEICLITRNRQDWTERLSFL
                     AQAAKELPVDHVLLDGEVVALNENGTSNFQLLQNSLSTHTSNNLAYYVFDLLHVDGKD
                     LMPLPLLMRKEALAEIVKAKSSSSAIRYSEHWLGQGDRLYDEACRSGLEGVISKRADQ
                     PYRSGRSRDWVKTKCVQNQEFVIGGFTDPSGSRAGLGALLVGVHDERGRLIYAGKVGT
                     GFTRQSLGELRSRLDPLVSKAAPFVNPPRGAEARGVHWVKPELIGAVSFAEWTSDNML
                     RHPSFQGLREDKPPVPITRERISGTNNSIASNPDAAVEVAGIKLTHPDRILYPEQGIT
                     KRELALYFEQSADWILPHVAGRPLTLVRCPEGRSKECFYQRHVTDSASEWIRPVPIKE
                     KGKLVNYVAVDNLQGLIALVQMGVLELHTWGCTIDNIDRPDRLIFDLDPDPEISWKPL
                     TRAAESLRAYLDDFGLSSFVKTTGGKGLHVVVPLVPKSDWGTAKEFSKRVAQQMVRRA
                     PERYTATMSKAKRGGKIYIDYLRNAKTATAVSAYSTRARAGAPVSMPVGWDELANDVR
                     GEYFNLRNTAERLSRSGKGPWHGYETARRAITAAMIKQVQSEDGSD"
     gene            6830..7555
                     /locus_tag="VF182_01085"
     CDS             6830..7555
                     /locus_tag="VF182_01085"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6435692.1"
                     /translation="MKDTVLFLTGAAFGAGMMYALDPDAGRRRRALTRDQLVRARIKT
                     RETASATARDLLNRSYGVMAGARSRLFQGEVDDQVIEQRVRSKFGYVVRNPAAIEARV
                     DSGRVSLIGAVRADEVDQLIAEVSAVSGVRAVENHLDVHRNADHVPELQGGDKPKPSP
                     RRLDIYHRRWSPATRLLIGISGAAIVYAVARPHFLWSALLSLGLVGYASAGADAGRAL
                     LPRRTKGKQLNALYDPLSTGWSS"
     gene            7713..9458
                     /locus_tag="VF182_01090"
     CDS             7713..9458
                     /locus_tag="VF182_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6435693.1"
                     /translation="MITAADVLIEAIQDWGVDVIFGLPGDGINGIMEALRKRQEQIRF
                     IQVRHEEAAAFMACAYAKYTGKLGVCLATSGPGGIHLLNGLYDAKLDGQPVLAITGHH
                     YHDLIDTHAQQDVDLDRVFMDVTVYNTRIMGPAHVEQVTHLACRTALSYRGVAHINFP
                     VDLQEQEIKEHSKRNIPGHTSTIFSSKSGLPEEKDLRAAAEVLNAGKKIAIMAGQGAL
                     AATEELEELAGTLAAPIIKPLLGKAAVPDDSPFTTGGVGLLGTKPSQEALEDCDTLLM
                     IGTSFPYIEFLPKPGQARGVQIDINGARIGLRYPVEVGLVGDSRLTLRQLIPMLKRNE
                     ERSFLKKAQDGMIEWWKLMEERGTRSDKPMKPQVVAWELGKRLTSNAIVSADSGTIAT
                     WFARQIRVQRGQKYSLSGTLASMANGLPYTIAAQIAYPERQCIGFVGDGGFSMLMAEF
                     ATCVKYRLPVKIVVIKNNVLGMIKWEQMVFLGNPEYGCELQPIDFAAFARACGGSGYT
                     IEEPASCGAILDQALAMPGPVIIDAVVDPFVPPMPPKITLTQASHFAEALARGEPNRS
                     KIAWTVLSDRVRELI"
     gene            complement(9450..9659)
                     /locus_tag="VF182_01095"
     CDS             complement(9450..9659)
                     /locus_tag="VF182_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435694.1"
                     /translation="MNDYGVLFIQARDRFLAELCQRIIDRARQMRPGKLLGRQNFNEM
                     CAFIDSLLQLVAAYNDLRHRPSSSN"
     gene            9811..10194
                     /locus_tag="VF182_01100"
     CDS             9811..10194
                     /locus_tag="VF182_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6435695.1"
                     /translation="MGHGSDAGRSADMSARRTCWAGRRASGGAGLRSFTGGQRKWHRT
                     RRAGTRDWETDRTASAEQLMDPAPTTLRPSDPLEKAKQMLGESDRGAVLVTDSDGKLL
                     GAFFGANDDQEPGRKQELPDTEVWS"
     gene            10286..11155
                     /locus_tag="VF182_01105"
     CDS             10286..11155
                     /locus_tag="VF182_01105"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX6435696.1"
                     /translation="MKRAPRAGWINGLFVIGEFCLLLWLEHRRPLRRSVEPKLRRGAR
                     NLSVAAVSGLALQATERPLIGALVAIVERRRWGLLKRWSMPRRVEAALAVALMDYTLY
                     LWHVLMHRSHWLWRLHLAHHVDLDLDASTALRFHFSELLVSIAWRAGQVALIGVSPPA
                     YSAWQKLLMFSILFHHSNVDLPIELERWLSRVIVTPRLHGIHHSIVRDETDSNWSSGL
                     TLWDWLHGTLKLNVPQQAIIIGVPAYQRSEDVSLPKIIMMPFTRQPPSWQMPENGEPR
                     REALPAPANCLAQ"
     gene            12099..12839
                     /locus_tag="VF182_01110"
     CDS             12099..12839
                     /locus_tag="VF182_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX6435697.1"
                     /translation="MISIIIPARNDAVALQHTLDRLQQLTGIAGVQILVAASGDLGST
                     ERAVAGRAQLIRPARSSRSALMNTAADVASGEILFFLHADSFPPPDALERINSLLQNP
                     KILGGAFEHRFVETSWSLRAISWINRRRYRLTRNYYGDQGIFVRASVFRNLGGYRDLG
                     LMEDLDLSQRLKRSGRTVLIRSPLVTSGRRFIDRGPWRTFAFIVWLLFLYTLRLDTQR
                     YARRWQGPPDGVPGTRWIRSDGESNEVR"
     gene            12826..13080
                     /locus_tag="VF182_01115"
     CDS             12826..13080
                     /locus_tag="VF182_01115"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6435698.1"
                     /translation="MRLGEGAVLITGSTGFIGRHVTRRLYYSRRRVIALGRSQGGVSG
                     TKRLESLVGKPAGGNGLQVIESDLTQPSGGLRSSEITRLR"
     gene            13344..13724
                     /locus_tag="VF182_01120"
     CDS             13344..13724
                     /locus_tag="VF182_01120"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HEX6435699.1"
                     /translation="MVQGLTGFVKERSLNASHFTAIGAFNSAVLGFFDREEQDYKRIP
                     IREHVEVLSLVGDVALRGREPQLHAHVVVGKADGTAHSGHILQAYVWPTLEVVLTESP
                     DYLCRATDDETGLALIDLSDVKSG"
     gene            13844..14203
                     /locus_tag="VF182_01125"
     CDS             13844..14203
                     /locus_tag="VF182_01125"
                     /inference="COORDINATES: protein motif:HMM:NF015720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HEX6435700.1"
                     /translation="MKRLVWSNLLLGLWLMVSPFALVFANQRIFGVLWEDLILGFGIA
                     TFSLCRIVSRRKGEIVLADWVVMVLGCLTLINPFLYSYNNAPIAKWNNLAIGGTVFVM
                     AVYQDWKDEKATTPRNC"
     gene            complement(14304..16094)
                     /locus_tag="VF182_01130"
     CDS             complement(14304..16094)
                     /locus_tag="VF182_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX6435701.1"
                     /translation="MPSRIEDYAMIGDCHTAALIARDGSIDWLCFPRFDGSACFSALL
                     GNEEHGRWLISPAGTIKRIQRQYRPGTLVLETDYETADGAVTLIDCMPPRSREPDLVR
                     MVVGRRGQVRMQMQLIIRFDYGSIVPWVRRTEYGIRAVGGPDTLDLQSAVPLRGENYT
                     TEAQFTVAEGERVPFVLMWHPSHESTHSAIDAEQVIMHTEGWWKDWSDRCTYSGPWRE
                     AVLRSLITLKALTYAPTGGIVAAPTTSLPEQLGGVRNWDYRYCWVRDATFSLYALMLA
                     GYTDEACAWREWLIRAVAGKPSQLNIMYGLAGERRLTEMELAWLPGYEGAAPVRTGNA
                     AWQQFQLDVYGELMDTMHVARRAGLDPNENAWRVQLALMDFLESCWRHPDEGIWEMRG
                     PRRHFTHSKIMAWVAIDRMIKGVERFGLEGPVERWRALRETMQQEILHNGFDRERNTF
                     VQYYGGKDLDASLLMIPLVGFLPASDPRVQGTVEAIERELVIDGFVLRYPTKEHVDGL
                     PPGEAAFLACTFWLADNFAMLGRKEDALKLFERLLELRNDVGLLSEEYDPGTRRLLGN
                     FPQAFSHISLVNTAYNLSRVTGPAKDRQQS"
     gene            complement(16486..16692)
                     /locus_tag="VF182_01135"
     CDS             complement(16486..16692)
                     /locus_tag="VF182_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435702.1"
                     /translation="MEPSRIRAVDVFDRIQRGEETVLIDTRSPKVWAASDQKLPGALR
                     IPTDEIELHTHKLLRDRLIVTYCS"
     gene            17165..>17557
                     /locus_tag="VF182_01140"
     CDS             17165..>17557
                     /locus_tag="VF182_01140"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX6435703.1"
                     /translation="MLRSANELKGYTIEATDGDIGEVIQFYFDDEKWTIRYLVADTGG
                     WLMGRRVLISPAALGKVDWNSRKLHVNMNKERVENSPGVDTDRPVSRQHSLDGYQKAW
                     SYSYELIILDVRMPGLLGTEIAAALKQKN"
BASE COUNT         3819 a         4799 c         5144 g         3795 t
ORIGIN      
        1 ggccggggct ggcgcgtcca ccgcatgacc gaccccggcg tctaccgcgc gctgaccgct
       61 ttattgcttc tctcaccgca aacgccgatg ttgtttcaag ggcaggagtt cgccgcttca
      121 actccgtttg cgtattttgc cgatcacgaa ggcaagctcg ctgaaaaagt aaaagagggc
      181 aggcgcgaat tcttgcatca gtttcccagc ctcgccgcag aggcaatgca acgggaaatt
      241 cccgacccca cggatccgcg agtttttgcc tcgtgcaagc tcgacttttc ggaacgaagt
      301 cgccaccacg agatctatgc gctgcatcaa gatcttcttc gtctccggcg cgccgaccct
      361 gcctttaact cgcaagagcc gggggcattg gacggagccg tgctcggcgc tcgcgcgtgg
      421 gtattgagat tctttgcgcc ggaggggaaa gatcggctgc tgctgatcaa ccttgggatc
      481 gatatcatgc ttggcccggt tccggagccg ctgcttgctc ctccgttcgg cgcgatctgg
      541 caggttctat ggtccagtga gaacccgcgc tatggcggcg ccggcgcgtt accgctggaa
      601 aatgaaaacg gctggttggt cattggcaag agcgccgttg tcctggcgga ttctcccaaa
      661 tgactcctcc tcttgtgcgt acgctcaccg acgtcccgca gataaagctc gagccaccgg
      721 agcttactca tcatcccgaa tggctcgtga ccaatgggct tggcggttat gcctcaggaa
      781 cagtatccgg ggctatgacg cgacgctatc acggtctgtt gatcgccgcg tttccggcgc
      841 ccctcggaag aatgctcctg ttaagcgatc tcaacgaaga ggttcacttt cccaacggcc
      901 gctttgtcca gctgagtacc cgctatggtg attcggaacc tactgcaaga ttgattgagt
      961 ttcgactcga aatgggtttg ccggtttggc gttatcaaat cgatgattgg atgctggaaa
     1021 agcggcttta cctgccgtac cgccagaata cggttttcat cacctacaaa ccattggctt
     1081 gccccgatca tattcgactc aagctgcggc cactgattca gtatcgcggc catagcgatg
     1141 acgtcgatgc gcagccggcg gacccttacg ttctaaccgt attggaagat caatatcaga
     1201 tatcgacggg aaacctgccg ccgttacgcc tgctaccgta cggtaaaggc gaattcgtca
     1261 tcgatcgaaa aaagatcgag aaggtacgtt acttgcgcga agaacagcgc ggctatcatg
     1321 ttattggcga gctctggagc cccggcacct ttgtcctcca ggcagccgcc ggcgaggaga
     1381 gcacgctagt cgcatcggcg cagtcctggg aaatcatgcg cgctatgcgc cccggcgaag
     1441 cgctcgcagc ggaacgcgat cgccgcaccc gcctgattac atccgccgac aacagagctc
     1501 attcggggtt cgccgccgag ttggtgctgg cggcggatca attcatcact gtgccggcgg
     1561 gccgtctagc cgatcagacg cgaacccatg ctgcaggcga cgaaatgcgc actgtgatcg
     1621 ctggttatca ttggttcacc gactggggac gagacaccat gatcagtttg gagggcttga
     1681 cgcttgtcac ccgtcgtttt ctcgaagccg gctggattct gcgcaatttc gccaaaagta
     1741 ttcgacacgg gctgatcccc aatctatttc cagagggaag ccgtgaaggc ctttaccata
     1801 ccgccgatgc gactttatgg ttttttcatg caatcgagcg ttacgttcaa gccacgggcg
     1861 atcgctggac tcttagattc ctgttaccca aactgattga aattttcgag gcgcatatcc
     1921 gtggaactct gttcggcatc agcgtcgatc cgcaggacgg gctattgcgc caaggcgaag
     1981 aaggttacca actcacgtgg atggacgcta aagttggcga ttgggtggtc acgccgcgcc
     2041 gtgggaaggc ggttgaaatc gatgcactct ggtacaacgc cctgcggctt ctgcagaaat
     2101 gggtcgaaga ggaagaagac cggaagttgg cgcgtgagat aggagcttgg gccgacaaag
     2161 ctagaaactc attcaataaa aaattttggt gcgccgacaa gggttatctt tacgacgtca
     2221 tcgatggtcc tcatggcaat gatgctgcct gccggccgaa tcaattgctc tccatctctc
     2281 tggatcatcc gattctcgaa tcagaccgat ggaagccggt tctgagaacc gttcaagagc
     2341 gtcttctcac tccgttcggc ctgcgctcgc tcgccgcaac tcatgcggat tacaagactc
     2401 gatatttcgg cgacattcgc gcgcgcgacg cggcctatca tcaaggaacg gtctgggcct
     2461 ggttgattgg cccgtttatc gatgcctggc tcaaagttta tcccgaagac cgatccgggg
     2521 ctcacgaatt tttgcgaggt tttattccgc agatgaatga gcacggcatc ggtacgatca
     2581 gcgaggtttt cgacgcggaa caaccatgga ccccgcacgg ttgtatcgcc caggcgtgga
     2641 gcgttgctga ggtgctgcgc tgctggatca agacagctcc atgatcgtgt tcaaagcaga
     2701 agttgaaagt ggaagttgaa ataacttcga tgccgaaatc gtcagccaat gtaccgcggc
     2761 ggcagtattc agctttccgt ttcagacttc ctcatttcat ccctgtcccg ctatcgcagg
     2821 attcactgcc cgcttgtccc agaattagaa tacagccagc caagctgttg gcattcgcag
     2881 cgaatatggc agcataaact tctgtttttt ggcatatgct ttgcggcctt ctttcgggaa
     2941 ggaggtgttt ccctatgcag atcagagaca tcatgacgag agacgtcgaa gtcgtgtcga
     3001 tcgatgctcc ggtgcgcgaa gccgcggcga aaatgaaaca gctcgacgtc ggcgccatcc
     3061 ccgtgtgcga cggacagaaa ttaaccggtg tgctaacgga tcgtgacatc gcggtgcgtc
     3121 tggctgcgga gggacgcgat ccgtcggaaa ctcgggtgtc cgagattatg actcgggatc
     3181 tttattattg cttcgaggat caagatgtcg aagaagcggc tacggtaatg gaagctggcc
     3241 agatccggcg gctgccgata ctcgatcacg accggcaact ggtcgggatt gtttctctgg
     3301 gcgatatctc agttcgatcg gatgaaaaag aggcagcggg cgaagccctc gagggaatct
     3361 cagagccggc ggcgccgaaa cgatgatggg tcacaactct gttgtggcgt tgcacagatc
     3421 tttttagcgc gactacacag gctggcaagg agccaatttc aactcaatca tcgagaagga
     3481 tacatatgac tgctgatcgg cccaagcgtg ataaaaaact gggctccggg cgcgttcgta
     3541 cgtcgggtga aaggacgcgc ggcgagaccg tcaaagcaaa acccaaggat gaacacgcct
     3601 gcagaactaa aaacctgaaa ccgcgccggg tttagcagga tggctcgttc aagtaaagcg
     3661 gctcccaaga agaagacacg gtcggcaagc aagcctgccg ggcgcgccct gtggaaaggc
     3721 tcgattaatt tcggcctggt gaatataccc gtggcgcttt atcccgctga aacatcgcaa
     3781 tcgttggatt tagatatgct tgatcggcgt gacttctcgc cgatcaagta tcaacgcatc
     3841 aacaaaaaga ccggcaagga agttccctgg gatcagatcg tgaagggttt cgagtacgaa
     3901 aaaggcgaat acgtgactct tactgacgag gaattgcgcc gcgccaatgt cgaggcaact
     3961 caatcggtag atattctcga tttcgtcgga gccgccgaga tcagcccggt ctattttgac
     4021 aagccgtact acttggaacc gcaaaaaaac gggcggcgcg cctacatgct cttacgtgaa
     4081 gtgttaacca aatccaataa agtcgccatc gccagagtga tcattcgcac ccgcgagcat
     4141 ctcgccgcgg tgatcccgca gggccctgtg ctgattctca acttgcttcg gtttcaacat
     4201 gaactgcgcg atagcaaagg gctggatatt ccggagacgg gccgcaaagg gcgggccatc
     4261 agcgccaacg aactgcggat ggctgagcgt ttgatcgacg ccatgtcggc gccctggaat
     4321 ccggcaaagt atcgcgacga ataccgtgaa gacgtggaga atctgattga aaagaaaatc
     4381 aaaagcggcc ggccaagaac ggtctcaggc ggcggtaaag cctcggcgcg taaaaccggc
     4441 ggcaaggtgg ttgacataat gagtttgctc cagcgcagcg tggttcaggc tcagaagaag
     4501 gataatgctg cacggcgtaa ggcgagctga agtctccgcg caaaatgaaa gatgaaaact
     4561 gaaagcgacc gaggcccgtt ttaaacgaag aatcggggct tcgataggtg ttaatgatgc
     4621 ggctcggccg gaattggcta tcccaggcct ctttttttga cgccgccggc agatgtcagt
     4681 tggatcctac tcactggatc tgaaggtctt gaagcgcgtg taagcgcata gaacaaagca
     4741 cttgcggctc atttgtggcg gcacgttttg ctctcccgaa cgatgaaacg caataatccc
     4801 aatacgacag cgagagcgga ttccgaacag gaagccgacc tgcgcggcac gcggcgcaaa
     4861 gcgatcaaaa aatctccccc atcgccggtc gcccgagcgc ggaagtctcg gctgccggac
     4921 ttcgtggcgc cgcagttggc aacattggtg gatcgcgtgc cccagggcga cgagtggctg
     4981 cacgagataa agttcgatgg ctaccgaatc ctgtgccgga ttagcaatga cgagatttgc
     5041 ttgatcacgc gtaaccggca ggattggacc gaacggctga gtttcttggc gcaggctgcg
     5101 aaagaactgc cggtcgatca cgtgctcctc gacggcgaag tcgtggctct caatgaaaac
     5161 ggcaccagta actttcagct tctgcaaaat tcgctttcga cccataccag caacaactta
     5221 gcctattacg ttttcgattt gcttcatgtg gatggcaagg atttgatgcc gctgccgttg
     5281 ctgatgcgca aagaagctct ggctgagatc gtcaaggcga aatcgtcttc ctccgcgatc
     5341 cgttacagcg aacattggct cggccagggc gatcgcctgt acgatgaagc ttgtcgctcc
     5401 gggctcgagg gggtgatttc taaaagagcc gaccagcctt atcgttcggg ccgcagtcgc
     5461 gactgggtca aaaccaagtg tgtccagaat caagagtttg tcatcggcgg cttcaccgat
     5521 ccgtcgggat cgcgcgccgg gctcggcgct cttctcgtcg gggttcacga tgagcgcggc
     5581 cgtttgattt atgccggcaa ggtcggcacc ggattcaccc ggcaaagtct cggtgaattg
     5641 cgcagtcgac tggacccgct cgtttccaaa gcagcgccgt ttgtcaatcc accccgaggc
     5701 gcggaggcaa gaggcgtgca ttgggtcaag ccggaactca tcggcgccgt ctcgtttgcg
     5761 gaatggacca gcgataacat gttgcgtcat ccgtcatttc aaggattgcg cgaagacaaa
     5821 ccgccggtac cgatcacgcg ggagcgcata tcaggaacaa acaattccat tgcgtccaac
     5881 cctgacgcag ccgtagaggt cgccggcatt aagctgactc atcccgatcg cattctctat
     5941 ccagagcagg gcatcaccaa acgtgagctc gctctttact tcgagcaaag cgccgattgg
     6001 attctgcccc atgttgcagg caggccattg acgcttgtcc gctgcccgga aggccgaagc
     6061 aaggagtgct tttatcagcg gcacgtcacc gactcggcca gcgaatggat ccgtccggtg
     6121 ccgatcaagg aaaaaggaaa gctggtgaat tatgtggcgg tggataatct ccagggactg
     6181 atcgcgctgg tgcaaatggg agtgctggag ctgcacactt ggggctgcac catcgataat
     6241 atcgatcgac ccgatcgtct gatcttcgat ctggatccgg acccggagat ttcttggaaa
     6301 cccctcacgc gggcggcgga gtcattgcgc gcctatcttg atgacttcgg attatcgagc
     6361 ttcgtcaaaa ccaccggcgg caagggactt cacgttgttg ttccgcttgt cccgaagagc
     6421 gactggggca ctgccaaaga attttccaag cgggttgcgc agcagatggt gcgccgagcg
     6481 ccggagcgtt ataccgcgac tatgtcgaag gccaagcgcg ggggaaaaat ttacatcgac
     6541 tatctacgga acgccaagac cgcaacagcc gtctccgcct attcgactcg ggcgcgagcc
     6601 ggagcgccgg tgtccatgcc ggtcgggtgg gacgagctag cgaacgatgt tcgtggagag
     6661 tatttcaatt tgcgcaatac agccgagcgc ttgtcccggt ctgggaaagg tccatggcac
     6721 ggatatgaaa ccgcgcgtcg cgcgatcaca gcggcgatga tcaaacaagt gcaatcggag
     6781 gatggcagcg attagctcgc cgatcgcaag aagaagtgaa ggaggtcgta tgaaagacac
     6841 tgtgttattt ctcaccggcg ccgcgtttgg agccggtatg atgtatgcgc tcgatcccga
     6901 tgccggccgc cgtcgccgcg ccttaacacg cgaccaatta gtcagagctc ggatcaagac
     6961 ccgcgaaacc gcatcggcta ccgcccggga tctcctgaat cgcagctatg gcgtgatggc
     7021 tggggcccgc tcccgcttat ttcaaggcga ggtcgacgat caagtgatcg agcaaagagt
     7081 tcgctcgaag tttggctatg tggtgcgcaa tcctgcggcc atcgaagccc gcgtcgactc
     7141 gggacgggtg agtttaatcg gtgcggtgcg cgctgatgaa gtcgatcaac tgattgccga
     7201 ggtctccgcc gtaagcggcg ttcgcgccgt cgagaatcat ttggatgtgc atcgtaacgc
     7261 ggatcatgtg ccggaacttc aaggcggcga taaacctaaa ccaagtccgc ggcggcttga
     7321 catctatcac cgccgctggt cgccggcgac gcgcctgcta atcgggattt ccggcgcggc
     7381 gatagtctat gccgttgccc ggccgcattt tctctggagc gctttattat cgttggggct
     7441 ggtcggttac gcctcggccg gcgctgatgc gggccgcgcg ctgttgccgc gccgtaccaa
     7501 aggaaaacag cttaatgcgc tgtatgatcc tctgagcacg ggctggagta gttgagcatc
     7561 tcgcctggga ttgatttatg tttgatgatg cggatgggaa cttccagtac ataagtctgc
     7621 cgtagaggtc ggtttgacct gcccctacaa ggcgtttgcg ccggcgcaag catctgcgtc
     7681 gtttgcgtcc tcagctctag aagggaagga tcatgattac cgctgccgat gttcttatcg
     7741 aagccattca ggattgggga gtcgatgtta ttttcggcct gcccggcgat ggaatcaacg
     7801 gcattatgga ggcgttgcgc aaacggcagg agcagatccg gtttattcag gtgcgtcacg
     7861 aagaagcggc tgcgttcatg gcctgcgctt atgccaaata caccggcaag ctcggcgtct
     7921 gcttggcaac ttcgggccct ggcggcatcc acttactgaa cggactttat gatgccaagc
     7981 tcgacggcca accggtgctg gcgatcaccg gccatcacta tcatgatctg atcgatactc
     8041 atgcgcagca ggacgtcgac ctcgaccgcg tgttcatgga cgtaaccgtt tacaacacgc
     8101 ggatcatggg accggcgcat gtcgagcagg tgactcatct agcatgccgc accgcgctca
     8161 gttatcgggg cgtggcccat atcaacttcc cggtcgacct gcaggagcag gaaatcaagg
     8221 agcactcaaa gcgcaacatt ccggggcata cgtcaacaat attctcgagc aaatccggtt
     8281 taccggaaga aaaagatttg cgcgccgccg ctgaggttct caatgccggc aagaaaatcg
     8341 cgattatggc ggggcagggc gcgttggcgg ctaccgagga gctggaagaa ctagccggca
     8401 ctctggccgc gccgattatc aaaccactac tgggcaaagc ggcagtgccc gacgatagcc
     8461 cgtttaccac tgggggagtc ggcctgctcg gcaccaagcc gtcacaggag gcgttggagg
     8521 actgcgacac cttgttgatg atcggcacgt cgtttccgta tatcgagttc ctacccaagc
     8581 cggggcaagc gcgcggggtg cagatcgaca tcaatggggc gcgcatcggc ctgcgctatc
     8641 cagtcgaagt ggggctggtc ggagacagcc gtctcacctt gaggcagttg attcccatgc
     8701 tgaaacgcaa cgaagagcgg agctttctaa aaaaggccca ggacggaatg atcgagtggt
     8761 ggaagctgat ggaagagcga ggcacccgaa gcgataagcc gatgaagccc caggtggtcg
     8821 cctgggagct gggcaagaga ttaacatcga acgccattgt ctcggccgat tcaggaacaa
     8881 tcgcaacctg gtttgcgcgc cagattcggg tgcaacgcgg ccagaagtat tcgttatcgg
     8941 gtaccctcgc gagcatggcc aatgggctgc cttatacgat cgccgcgcag attgcttacc
     9001 ccgagcgaca atgcatcggg tttgtcggcg atggcgggtt ttcgatgtta atggccgagt
     9061 tcgctacatg cgtaaagtat cgcctgccgg ttaaaattgt cgtgatcaag aataacgttt
     9121 tgggcatgat caaatgggag caaatggttt tcctgggcaa tcctgaatac ggctgcgagc
     9181 tgcaaccgat cgatttcgcc gctttcgcgc gcgcctgcgg cggctccggc tatactattg
     9241 aagagcccgc ctcgtgcggc gctattctcg accaagctct ggcgatgccg gggccggtca
     9301 tcatcgatgc ggtggtggat cctttcgtgc cgccgatgcc gccgaaaatc actttgacgc
     9361 aggcgagtca ttttgccgaa gccctcgcgc gcggcgaacc gaaccgatcg aagattgcct
     9421 ggacggttct atcggacaga gtgcgcgagc taatttgaag aggagggccg atgccgtaga
     9481 tcattataag cagcgacgag ctgcaacagc gagtcgatga aggcgcacat ctcgttgaag
     9541 ttttgccggc caaggagttt gcccgggcgc atctgccgcg cgcgatcaat aatccgctgg
     9601 caaagctcag ccagaaaacg gtcgcgagcc tggataaaaa gaacaccata atcgttcatt
     9661 gctatgactg ccagtgagac ctggctccta gagccgcgtg gcggctcgag agtttcggct
     9721 tcgaaaacgt cttctactat atacccggac gctcgattgg ctggcgcatg gcttgccggc
     9781 agtgagagag aatcaaagcc aggaatccat atgggtcatg gatcggatgc gggtcgaagt
     9841 gccgacatgt ccgctcgacg aacctgttgg gcaggccggc gcgcgagcgg aggagcgggg
     9901 ctgcgaagtt tcaccggtgg tcaacgaaaa tggcatcgta ctcggcgtgc cggtacacgc
     9961 gattgggaaa ccgaccgcac cgcatcggcg gaacagctca tggatcccgc gccgacgact
    10021 ctgcgcccca gcgatccact tgaaaaagcc aagcagatgc tgggcgagag cgatcgaggc
    10081 gctgttcttg tcaccgattc agatggaaaa ctgctgggcg ccttctttgg cgctaacgat
    10141 gatcaagagc ccggccgcaa acaggagctg cccgacaccg aagtgtggtc ttgatggagc
    10201 gcctatgcag tcagctcgat ccagccatcg atcagcaaat cttcacaggc gcggcgtgat
    10261 ctaacatcgg ggaaggcatc ttctgttgaa acgcgcgccg cgagctggat ggatcaacgg
    10321 gttattcgtg atcggcgaat tttgtctgct gctctggctc gagcatcgac ggccgctgcg
    10381 ccggtcggtt gaaccgaagc tcaggcgcgg cgcaaggaat ctttcagtgg cggcagtgag
    10441 cgggctggcg ttgcaagcca ctgagcggcc gcttatcggc gcgctcgttg ctattgtcga
    10501 acgccgccgg tgggggctgc tcaagcggtg gtcgatgccg cgccgggtcg aagcggcgct
    10561 ggcggtggcg ttgatggatt acacgcttta tctctggcat gtgctcatgc atcggtcgca
    10621 ctggctgtgg cggctgcatc ttgctcacca tgtcgatctc gatctcgacg cctcgacggc
    10681 gctgcgcttt catttcagcg agctactggt ttcgattgcc tggcgcgccg gccaggtggc
    10741 gctgatcggg gtttctccgc ccgcttactc cgcctggcag aaactattga tgttttcgat
    10801 cctgttccac cattcgaacg tcgatctacc gatcgagctt gagcgttggc tcagccgggt
    10861 tatcgtcacg ccgcgtttgc acggcattca tcattcgatc gttcgggacg agaccgactc
    10921 caactggtcg agcggattga cgttgtggga ctggttacat ggaaccctga agctcaacgt
    10981 gccgcagcaa gcaatcatca tcggcgtccc tgcgtaccag cggagcgaag acgtgagcct
    11041 accgaaaatt atcatgatgc cgttcacgag gcagccgccc agctggcaaa tgccggaaaa
    11101 tggagagccc agacgagaag ctttgccggc acctgcaaac tgtttggcgc aatgaaaaat
    11161 cggtatacga acagagatca tcgtttaaac tttagaccga gtctttgcca tgaaccttga
    11221 attactcgaa acccacagct tactgtgcag cacctacgcc aacagctcca gcgagaacac
    11281 acttccgatc tgaagcggcc ccgcagtatc atagcttggt cgcgggtcgg tatggcaagc
    11341 atgatcgcgg tgactttgtt gcagaccggc atctcaagca tcttcccgat cggccttttg
    11401 gcaacttcga ttccgatcgg gtgaactcgt cggataagct tatcgactag gagcgtcgga
    11461 tggctcgatt tcgcttgcga gtctagcggc caacattcga ttgcgccatt tggcggcgcc
    11521 aatcgcgtgc agagcaacct tgggttccgc tcgtcgcggc agtgaaagct gcggtgaggt
    11581 gctggcaatg agttggtttt ctatcagatg ccggtaaagg aaatgcgtga tgcggctact
    11641 gcattatctg cgcgcgggca aacaccgaca tcgcggctct tacgataccc gagccgcgaa
    11701 aagccatcgc tgttttgatc ggtgaaaacg gagtgaaact acaaaagcgc agtgatcgaa
    11761 ttaagcaccc atcgctgtga cacgacggag gagagatgaa aagacctgtg gagccggacc
    11821 cagtacggcc cgaaaaaacc tcgatgtcga acgcggagcc gataacagtg agagtgaaga
    11881 acgcccgcgc aggcaaaacc gcggcaggcg cgggtccggg ccggcgcgcg agccctcgac
    11941 gcgaaaagat cgcggccgtt cgtaattgaa gtttcccatg gaccaagcga ttcattccag
    12001 atcgtcaaca accgggggaa gttttccctt gcgaacgtct tccccactaa ctcacgcacc
    12061 tgaaggctgg ctcttacctc gcctagaaag tgtgtccgtt gatctcgatt attattcccg
    12121 cgcgcaatga tgccgttgcc ttgcaacaca ccctcgatcg tttgcagcag ttgactggga
    12181 tcgccggcgt ccaaatcctt gtagccgcgt cgggtgactt gggatctacc gaacgagcgg
    12241 tagcgggccg cgcgcaactg attcggccgg cgcgttcgag ccgttcggct ctgatgaaca
    12301 cggccgccga tgttgcaagc ggcgaaatcc tgtttttcct tcatgcggac tctttccccc
    12361 cgcccgatgc gctggagcgg ataaactcgc tgctgcaaaa cccaaaaatc ctcggcggcg
    12421 ccttcgagca ccgctttgtc gaaacaagct ggagcctgcg cgcgatcagt tggatcaacc
    12481 ggcggcgcta ccgcctcacg cgcaactact atggtgatca gggaatcttc gtgcgcgcaa
    12541 gcgtttttcg aaatctcggc ggctatcgcg atcttggctt gatggaggat ctcgatctga
    12601 gccagcgctt gaagcgcagc ggccggacgg tgttgattcg cagcccgctg gtgacttccg
    12661 gccggcgatt tatcgatcgt ggcccgtggc gtacgttcgc ttttatcgtc tggttgcttt
    12721 ttctttatac gctgcggctt gatacgcaac gatacgcgcg ccgttggcaa gggccgcccg
    12781 atggagttcc cggcactcgg tggatccgat ccgatggaga gtcgaatgag gttaggtgaa
    12841 ggcgccgtcc tgatcaccgg aagcaccggc tttatcggcc gccatgtcac gcgccgcctg
    12901 tactacagtc gacgacgggt gattgcgctc ggccgcagcc agggaggtgt ctcggggaca
    12961 aagcgattag aaagtcttgt gggcaaaccg gctggcggaa acggcctcca agtgattgag
    13021 agcgatctca cgcagccgag cggtggtctt cggtcgtctg agatcacgcg cctgcgctaa
    13081 cgtgcaaacg gtcattcatt gtgccggcga caccagtttc tttccacaaa cgacagcggc
    13141 ctcacgcgca atcttcatcg atgcgcctct ggcgttgctc caggcgctct ttccggggcg
    13201 gcttgagcgc tggtgccaga tttccaccgc gtttgtttgc ggccggcgtg gcggcgccgt
    13261 cgacaggagg tctcgatgaa aagcaagttg atttatgacg ggtcgcagaa aacccttgtg
    13321 ctggtattcg aaagggcgat gaattggtcc aagggctcac tggattcgtt aaggagcgca
    13381 gcctgaacgc gagtcatttt acggccatcg gagcattcaa tagtgccgtc ctcggttttt
    13441 tcgacagaga ggagcaagac tacaaaagga ttccaatcag agagcatgtg gaggttcttt
    13501 ctctagtcgg cgatgtagcg ctgcgcggca gagaaccgca gcttcatgcg cacgtggtcg
    13561 taggcaaggc cgatgggacg gctcatagcg gccatattct tcaagcctac gtgtggccta
    13621 ctctggaagt ggtgctgact gagtcaccgg attacctgtg ccgcgcaacc gatgatgaga
    13681 cggggctggc cctgatcgat ctatccgacg tgaagagtgg atgaccgtta ccttttgtct
    13741 ctcctacatg gccggcaaat ttttgatagt attaatagtg aggacaagtt gtggcggctg
    13801 ccggccgtcc gcttcctttg aataccgtgg gaggtgactg gatgtgaaac gtttggtttg
    13861 gtcaaaccta ttattaggat tgtggctaat ggtttcgccg tttgctctgg tattcgcaaa
    13921 tcagcgcatc ttcggcgtac tttgggaaga cttgatactg gggttcggca tcgcgacctt
    13981 ttcgctctgc cgcatcgtgt cccggcgcaa gggcgagatt gttttggccg actgggtggt
    14041 aatggtgctt ggttgtctga ccctgatcaa tccgttttta tacagttaca acaatgcgcc
    14101 aatcgccaaa tggaataatc ttgccattgg tgggacggtc tttgttatgg ctgtgtatca
    14161 agactggaag gacgaaaagg ctacgacacc tcgtaattgc taatttcgag aaaacactaa
    14221 aacaggcatc aaccaattta tcggtggcag ggagtcatgc acgaagcgat tgccttgagc
    14281 gaaggccaat ccaatcgggc ttatcagctc tgttggcggt ccttggccgg ccctgttact
    14341 cgcgaaagat tataagccgt attcaccagt gagatgtggc tgaatgcttg gggaaaatta
    14401 ccgaggagcc gtcgtgttcc cggatcgtat tcttcggaca gcaaccccac gtcattgcgc
    14461 agctctaaaa gccgctcgaa gagttttaaa gcgtcctctt tccggccgag catcgcaaaa
    14521 ttatccgcca gccagaaagt gcaggcgaga aaggcggctt cccctggcgg caggccgtcg
    14581 acgtgttctt ttgtcggata gcgcaagacg aaaccatcga ttaccagctc gcgctcgata
    14641 gcctcaaccg tgccttgtac gcgcgggtcg cttgccggca aaaaacccac gagcggtatc
    14701 atcaagagac tagcatcgag atctttccca ccgtaatatt gcacgaaagt attgcgctcg
    14761 cgatcgaagc cgttgtgcaa gatttcttgt tgcatggtct cgcgaagggc gcgccagcgt
    14821 tccaccggcc cttccagacc aaagcgctcg actcccttga tcatgcggtc gatggcgacc
    14881 caggccatga ttttggagtg cgtgaaatgc cggcgcgggc cgcgcatctc ccagattcct
    14941 tcatcaggat ggcgccagca ggattcgaga aaatccatca gcgcgagctg gacccgccag
    15001 gcgttttcat tgggatccag tccggcgcgg cgcgccacgt gcatcgtgtc catcaactcg
    15061 ccgtagacat ccaactggaa ttgctgccat gccgcgttgc cggtccggac cggagcggcg
    15121 ccctcgtagc cgggcagcca ggctagctcc atctcggtca acctgcgctc gccggccaag
    15181 ccgtacataa tattgagctg cgacggtttg ccggctaccg cacggatcag ccactctcgc
    15241 catgcgcacg cttcatcggt ataaccggcc agcatcaaag cgtacaacga aaacgtcgca
    15301 tcccgtaccc aacagtaacg atagtcccag ttgcgaacgc cgccgagctg ttcgggcagc
    15361 gaagtggtcg gcgccgcgac gatgccgccg gtaggcgcat aggtgagcgc tttgagcgtt
    15421 atcagcgatc gcaaaacggc ttcgcgccat ggcccagagt aggtgcagcg gtccgaccag
    15481 tctttccacc agccttcggt gtgcatgatg acttgctcgg catcgatggc tgaatgcgtc
    15541 gattcatgtg agggatgcca catcagcaca aagggaactc gctctccttc agccaccgta
    15601 aactgagctt cggtcgtgta attttcgccg cgcagtggca ccgcgctctg caggtccaag
    15661 gtatcggggc cacccaccgc gcggatccca tactcggtac ggcgcaccca gggcacaatc
    15721 gagccgtagt caaaacgtat gatgagctgc atttgcatgc gcacttggcc gcgtctgccc
    15781 acgaccatcc gcaccagatc cggttctcga ctccgcggcg gcatgcaatc gattaaggtg
    15841 acagcgccgt cggcggtctc ataatcagtt tcgagcacca gggttcctgg gcgatattgc
    15901 cgctggatcc gtttgatcgt gcctgccgga gaaattaacc agcggccatg ctcttcattc
    15961 ccgagcagcg ccgagaaaca ggcgctacca tcgaagcgtg gaaaacagag ccaatcgatc
    16021 gatccatcgc gcgcgatcaa agcggcggtg tggcaatcgc cgatcatagc gtagtcttca
    16081 attcgtgatg gcatcagtaa ccattccggg aaactgccgg tcagcttccc actgcgttgc
    16141 gcgttggttg ctccaattcg tgttctgtcg tgagtacttg ttccttcgaa tttgcggaaa
    16201 tcgtgatgag agacggcgcc tgtgcgactc aaccttgaga ccccattgag ccgcgcgcaa
    16261 ggtcgtaaac gcactattct actttactgc ctaaactaca gtgagaaaag atagtagctg
    16321 gaagccagat attttttaag gcaacgccga ggtaatggga ggcttacttt ccgtgggata
    16381 gcccgctttg tgccatgcct cgaatcctcc gtccaagacc gtcacgtctt tgaagccgac
    16441 atctcttagt cttcgtgccg cacgggcact cgaggcctct tgcggtcaag agcaataagt
    16501 aacgatcaat cggtcgcgca atagcttgtg ggtatgaagc tcgatttcat ccgtagggat
    16561 tcgcaaggct cccggcagtt tctgatcgga tgcagcccaa acctttgggc tcctggtatc
    16621 gatcaaaaca gtttcctccc cgcgctggat tcgatcgaaa acgtcaaccg ctcgaattct
    16681 cgaaggttcc atctcttacc tccgtaggtt tactctaggc tgagttgctt aaataactag
    16741 ctcgactggt tgcgcactat ttttaaaagg gttgcgcctc ttaaaagatc atgtcgcaat
    16801 cacaatgcca tggggctcct gttaaagcag caatcggtta ctgaccgcgc tcagagtctg
    16861 gctcgttatc ccccttggcg acttggttat gaaagtgtga ttccgacgat caatgagttt
    16921 attgtcgggg taacaatgat cgcgtttgca atgacgcgtg gaaaagcgcg cggtaaattt
    16981 ggcggctggc tggggcagtg tggtgaagag ggaactccaa gccagcgcta gaactcgttt
    17041 caagatgtcc cgacgctagt cgaccaggaa tggaatgcga atcgcgccaa ccgcatggcc
    17101 gacaagcgac gattcccggg tggcactgcg cttgctcgtt acgtaagcct aaggaggtga
    17161 tcacatgctt agaagcgcca atgagctcaa gggttatacg attgaggcga ccgacgggga
    17221 catcggcgaa gtgattcagt tttatttcga cgatgagaaa tggactatca gatatttggt
    17281 ggcggatacc ggcggttggc tgatgggaag aagagttttg atctcgcccg ctgctctcgg
    17341 caaagttgat tggaattcac gaaaactgca cgtgaacatg aacaaggagc gggtcgaaaa
    17401 tagtcccggc gtcgataccg atcggccggt ttcgcgccag cattcgctgg atgggtatca
    17461 aaaagcgtgg tcctattcct atgagctgat tattcttgat gtgcgtatgc cgggactctt
    17521 aggcaccgag attgccgcgg cgctaaagca gaaaaat
//
LOCUS       DASUVS010000021        10089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_442739, whole genome shotgun sequence.
ACCESSION   DASUVS010000021 DASUVS010000000
VERSION     DASUVS010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10089
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442739"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VF182_01145"
     CDS             complement(<1..453)
                     /locus_tag="VF182_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435704.1"
                     /translation="MKRVSFPQFLTCLMMLMPAIPALGAEGQAEKLRVAISSISTSQV
                     NIWVPFDTGFFKKHGLDIELVYISGAPVGAAALLSGEVAISQGGVVGSITSNMKGSGT
                     YIILGGADRFPYQLVVPPTIRQISELKGKRFAVSRIGSADHTATLFVLP"
     gene            complement(541..1473)
                     /locus_tag="VF182_01150"
     CDS             complement(541..1473)
                     /locus_tag="VF182_01150"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6435705.1"
                     /translation="MVCTLLTQRFVPAPRNTRESPASQFHLGSGEDWAAFVEKAGIDN
                     SVLFTSEGLSVGFIQLPAYAVSICRSYNDYVYERFASVSPRLKPMALIPMQDVPAAAQ
                     ELRRAVKELGFPGAMIPSTGLPLHLGHQLYWPIYEEAERLDCALAVHGGPARGMGLDT
                     FTSMVDAHVLHHPVPLMMALVSFIYDGVFDRFPNLRLGLMEGGCGWLPCVLDRMKRDQ
                     DHYQHFSGPRLHPVEYIAGGRVLIGVEDSEETLSYLVKRIGAQVFAYASDYPHEVDFV
                     AAKHEIQEMTERSDLTDEEKQAILGGNAKRFYKL"
     gene            1922..2563
                     /locus_tag="VF182_01155"
     CDS             1922..2563
                     /locus_tag="VF182_01155"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6435706.1"
                     /translation="MERTQFIIVRHGQTQWNFRGVRQGHLDSDLTERGIAQANALATR
                     LAREHFTRLYSSDLGRALRTAQIIAASTGHEIVTDARLRERHLGVFQGLSGDEIKARY
                     PEEHRLHRSVGPDYVIPGGESVRQQVARNVAYLTEIARQHAGETLVVVTHGGVLSGLF
                     RHTFSIPFDAPRRFEFTNAGLNVFSYRQGDWFLQTWGDTSHLCDVGTVDGDDP"
     gene            complement(2804..4264)
                     /locus_tag="VF182_01160"
     CDS             complement(2804..4264)
                     /locus_tag="VF182_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435707.1"
                     /translation="MSEIRFIDTTVRDGNQSLWALNMRVGAMLPIAEPMDQAGFESME
                     FFLSVMFKKYVREHKEDPWEWVREGTKRIKKTRLRYHGGMHSAFEKTPHCILKLLIER
                     LVSYGLTVTRTSNSWNDFADFKEEIADLKKSGMDTVANLIYSVSPRHTDEYYARKAKE
                     AAASNPWRICFKDVGGLLTPERARTLIPIVLKSAGEIPVEFHAHCNSGQAPLCYLEGV
                     KLGMRILHTAVPPLANGSSQPSIFNIAHNLRALGHTPMVDLKPLEAVEKHFTAVAKKS
                     GLPIGKPVEYDQSQYLHQVPGGMISNMRHQLKIVGMEHKMEAALEEAAHVRKDFGYPI
                     MVTPLSQYVGTQAAINVLLGERYKEVPDQVIQYALGFWGKEAVEVMDPDIRDKILSRP
                     RAKEWTHWEQPEPTLHQVRQKLGVHLSDEELILRFFAGDDYVNALPDGGKPHEHLNGD
                     LPLVKLIEQLSQRKGSNQIFIKRPGFSVRMEKRTTV"
     gene            4441..4860
                     /locus_tag="VF182_01165"
     CDS             4441..4860
                     /locus_tag="VF182_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX6435708.1"
                     /translation="MRSYRKELWFNTPTRVAFVNITGQVQGCIRESGVNDGLVLVNAM
                     HITASVFINDDESGLHHDYGVWLEKLAPHEPLKQYRHNDTGEDNADAHIKRQVMGREA
                     VVAITDGRLDFGPWEQIFYGEFDGKRRKRVLVKIIGE"
     gene            complement(4847..5752)
                     /locus_tag="VF182_01170"
     CDS             complement(4847..5752)
                     /locus_tag="VF182_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6435709.1"
                     /translation="MIEPVADLFRVTLLGTGAPPPLLGRFGPSTLIEAGDQKFIFDAG
                     RGAMQRLHQLGIPFADITGLLLTHHHSDHLVGFADLWLTGWIGRPWGSRKTPLHVWGP
                     HGTNQMMEHLPLAFAVDLRVRSRSYPMAGAKLESEEIKEGVVYSQNGIIITAFEVDHG
                     SEELPAFGYRIDYRGRSAVLSGDTTFNENLINHARGADLVVHEITAVSGTAAESPEQL
                     KRIGSNHTTPEQAGEVFARVKPKLAVYNHLLLFGGATAEDLIPATRKRYSGPLLVGED
                     LMRFDIGENIEAQLFHDSEASPTHR"
     gene            5791..7161
                     /gene="rlmD"
                     /locus_tag="VF182_01175"
     CDS             5791..7161
                     /gene="rlmD"
                     /locus_tag="VF182_01175"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX6435710.1"
                     /translation="MPVNLSSLEEFVQLTRVSLAGIVGSVKNNFNSGSPPASRTRSER
                     RSKSFKMRHEMRLEPAQPRRNQTSADGAYKSPCPHYPYCIGCPLSHVPYPEQLLKKRH
                     IVAGALARYPSLLDAEVPPVAAAPHRLGYRARIKLVVRSTRGEIATGLYVPGSHRVID
                     ISSCPVHPRPVNQVLQYLKKKILELGIEPYDERDDSGQLRYLDFRYSFAQRELSVTLV
                     TRHGDFPQGGALARSLSRKFSFINGVIQNVNPQRGNVIWGDRYRIISGRDTLLEQVGP
                     VTLGFPAGVFSQANPSTAKMLYETINAMAALNARETVLDLYCGVGPISLYLAGTARLV
                     WGVDDSSLSIDTAKQNARRNGISNCRFFEGDVAEKVAEAKKTLGQIDLIVLNPPRKGV
                     QPEAMEAMLAVNAPKLIYVSCNPDSLARDLDKLLRRHYRVRRLQPFDMFPQTEQVETV
                     VSLEKS"
     gene            7226..7669
                     /locus_tag="VF182_01180"
     CDS             7226..7669
                     /locus_tag="VF182_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6435711.1"
                     /translation="MAELKVDGVVHWSIPVNNLEEAEKFYGEILGLEPKGRLGGSGMS
                     CFKAGDHNILLCVRNETTVRTPQQDNRLHHAFMVSPEMWERGCRLFQKNGVKIAEPIV
                     YREKGHFTGRELYFLDPSGNMLELCDPTWKPGMPTPSYEEIVGTN"
     gene            8017..8991
                     /locus_tag="VF182_01185"
     CDS             8017..8991
                     /locus_tag="VF182_01185"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX6435712.1"
                     /translation="MLDDFLQILPEVIFEAAEKLGGRCTGRFYALNALENRVYDIEME
                     EGPHVVVKFYRPGRWSRAMIQAEHDFLKALDESEIPVVRPLTDANGQTINETNQVMFT
                     IFPKRPGRLEPELTTEQLMRLGKFLARLHNVGATVKAASRLRLDPQTYGRDPLKFLKD
                     GHFVPMELASRFETIATQICDAITPRFEGVEYVLLHGDCHSGNILWNRDDPYFIDFDD
                     MLYAPPVQDIWMLTGADDDYGREQRRILLQAYEQMRTFDIRTMHLIEPLRSLRMIHFA
                     AWIARRWEDSAFKAAFPDFGTEHYWQDQIEALALQLEKCENHTSDIYR"
     gene            9428..>10089
                     /locus_tag="VF182_01190"
     CDS             9428..>10089
                     /locus_tag="VF182_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435713.1"
                     /translation="MAFRKNLLALSFVLVWIGCASVTVAGAQLDSVTIGFSSFSGFYG
                     PLWLAVEDGLGKKYGVDLRAVYAGRVRPQQLLASGETPLVIASGSGAMTSHVLGVKDQ
                     VIVALLSSKMGGGIFSKSEIKRPEDLKGKLIATGRPGALNDVLVRYVLTRKWGLSPDR
                     DVKLMPIGEPPLMLQALERGVVDATSLSVPASFLAKSKGFFELVNYDALGLSYPQHAV
                     TTL"
BASE COUNT         2236 a         2720 c         2886 g         2247 t
ORIGIN      
        1 gggcaggacg aacagggttg ccgtgtgatc ggcggaaccg attcgcgaaa cggcaaagcg
       61 cttgcctttt aattccgaga tctgcctgat ggttggcgga accaccagtt ggtagggaaa
      121 gcggtcggcg cccccgagga tgatataggt cccggagcct ttcatgttgc tggtaataga
      181 gcctacgacg ccgccctggc tgatggcgac ttcgccggac aaaagggcgg cggcgcccac
      241 cggcgcgcca ctgatataga ccagctcgat gtcgaggccg tgtttcttaa agaagcctgt
      301 gtcgaacggc acccaaatgt tgacttggct ggtgctgatc gatgagatgg caacgcggag
      361 cttttcggcc tggccttcgg cgccaagcgc aggaatcgcc ggcatgagca tcatgagaca
      421 ggtcaagaac tgcgggaagg aaactctttt catcgggctc ctccttgact cggccgttcg
      481 agccgttcaa acaattcacg ccgttcaaga agttaagtca ggcgctctcg ccgctcagac
      541 tcacagtttg taaaatctct tggcgttgcc gcctaagatc gcttgcttct cctcgtccgt
      601 aagatcggat cgctccgtca tctcctgaat ctcatgcttc gccgcgacaa agtctacttc
      661 atgcggatag tccgaggcgt atgcaaatac ttgcgcgccg atgcgcttga ccaagtaaga
      721 caaagtctcc tcactgtctt caaccccgat cagcacgcgg ccgccggcga tgtattccac
      781 cggatgaagt ctcgggccgc tgaaatgttg ataatgatct tggtcgcgtt tcatacgatc
      841 gagcacgcaa ggcagccagc cgcagccgcc ttccatgagg ccgaggcgga gattgggaaa
      901 ccggtcgaaa acgccgtcgt agataaacga caccagtgcc atcatcagcg gcaccgggtg
      961 atgcagcacg tgagcgtcga ccattgatgt gaaagtatcg agtcccatgc cgcgcgcagg
     1021 accgccgtgc acggctagcg cgcagtcaag tctttccgct tcttcgtaga tcggccagta
     1081 aagctggtgg ccgaggtgca gcggcagtcc tgtagatgga atcatggctc cgggaaatcc
     1141 gagttctttc accgcgcgac gaagttcttg cgccgccgcc ggaacatcct gcatcggaat
     1201 caacgccatg ggtttgagac gaggactgac gcttgcgaat cgctcgtaga cgtaatcgtt
     1261 gtacgatcta cagatggaga cagcgtaggc gggcagctgg ataaaaccca cggaaagtcc
     1321 ttcggaggta aacagcacgc tgttatcgat ccctgccttt tcgacaaatg cggcccagtc
     1381 ttcgcccgat cctaggtgaa attggctggc gggtgactct cgtgtgtttc ttggcgccgg
     1441 cacgaatcgc tgagttagga gcgtgcagac catcgaggga cgggaacacg ccttcacgat
     1501 tgcggtagga gtgcagcgcg tggtctttcg gacctgcttc catgaactca gccatttcgg
     1561 cgatcgattc gaggatgtga ccgtcgcagt cggtcacgag aatttcagtt ctgacatata
     1621 ggataagctt cctcggtaaa agtaattcac cgttacaata gcggccgctt acctgtcaac
     1681 aggaaatagt ctcgactgag ttccatccga caagacgata tgcagttcgc tgacagaggg
     1741 agttggctca agccaaagca gtggcagatt cattggaatc agccaatcgg agggcgcggc
     1801 tcagcggagc tacatcggct cgccggaaat gaagcgacaa atctcccatc cggcgcgact
     1861 cataacattt gacattagta cgggcaggtg agactaagca tgaaccgcga ggttacaatc
     1921 gatggagcga actcaattta tcattgtgcg gcacgggcag acgcagtgga attttcgggg
     1981 cgtacggcag ggccacctcg acagcgatct gacggaaaga ggcattgctc aggccaatgc
     2041 attggctact cgcttggcgc gcgaacattt tacccgactt tacagcagcg atctgggacg
     2101 cgctctgcgc acggctcaaa tcattgccgc ttctaccggg catgagatcg tgaccgatgc
     2161 tcgcctacgc gagcggcacc ttggagtttt ccagggactt agcggcgacg agatcaaagc
     2221 caggtatccc gaagaacatc gcctgcatcg ttccgtcggc ccggattatg tgattcccgg
     2281 cggtgaaagt gtccgtcaac aagtcgcgcg caatgtggct tacctgacag aaattgcccg
     2341 gcagcatgcc ggtgagacgt tagtcgtggt cacccacgga ggcgtgctca gcggcctttt
     2401 ccgtcacacc ttttcgattc cgttcgatgc gccgcgccga tttgaattca ccaacgcggg
     2461 gttgaacgtg ttcagttatc gacagggcga ctggttcctg cagacttggg gagataccag
     2521 ccatctttgc gatgtgggta cggtggacgg cgacgatcct tagcacgcct gcacgctggt
     2581 gaaaacgtcc agctgctgcg ttgcgctcgt ccgactccgc tccggcgtct aacgcgagta
     2641 cgtctacgct tgccaaactt cgcgcgtctt gtatctgaac gtttgaccag cgtgcccaac
     2701 catcgaaaac gctaggcgct catcgtgatc gtgtccgtgc aaacgtgatc gagaactccg
     2761 aacgaacacg aacatgcgag cacgaagtcc gttcagcgaa gtgctagacg gttgtgcgtt
     2821 tttccatcct gaccgaaaag ccggggcgct tgatgaagat ttgattcgag cctttacgtt
     2881 ggctgagctg ctcgatcagc tttaccaaag gcagatctcc gttcagatgc tcgtgcggct
     2941 tgccgccgtc cggcagcgca ttgacgtagt cgtcgccggc gaaaaagcgc aagatcaact
     3001 cttcatcgga gagatgaacg cctaacttct gcctgacttg atgcagagtc ggttccggtt
     3061 gttcccagtg agtccattct ttggcgcgtg gacggcttaa aattttgtct ctgatatcgg
     3121 gatccatgac ctcgaccgct tctttgcccc aaaagcccag cgcgtattga atcacctgat
     3181 cgggcacctc tttatagcgc tcgccgagga gcacgttgat cgcggcttgg gtgccgacgt
     3241 actgtgacag cggagtcacc ataatcggat agccgaaatc ctttcgaacg tgagcggctt
     3301 cctcgagcgc ggcttccatc ttgtgctcca tgccgacgat cttgagctgg tggcgcatgt
     3361 tcgagatcat gccgcccgga acctgatgca gatattgtga ttgatcgtac tcgacgggtt
     3421 tgccgatcgg caagccggat ttcttagcca cggcggtgaa atgtttttcg acggcttcaa
     3481 gcggtttcaa atccaccatc ggggtatggc cgagagcgcg caggttatgg gcgatgttga
     3541 aaatcgacgg ctgcgacgag ccattggcca gcggcggcac cgcggtatga agaattctca
     3601 tgcccagttt gacgccttcg agatagcata acggcgcctg gccgctgttg cagtgggcgt
     3661 gaaattccac cggaatctct cccgcgctct tgagaacgat cgggatcaaa gtccgggctc
     3721 gctccggcgt gagcaagccg ccgacgtcct taaagcagat tcgccatgga ttgctcgctg
     3781 ccgcttcctt ggcttttcgg gcgtaatatt catcggtgtg acgaggggag accgagtaga
     3841 taagattggc gacggtatcc atgccgcttt tcttcaagtc ggcaatttct tctttgaagt
     3901 cagcgaagtc gttccaggag tttgaggttc gagtgaccgt aaggccatag gagaccagcc
     3961 gttcgatcaa cagcttgaga atgcagtgcg gggttttctc gaaggcgctg tgcatccctc
     4021 catgatagcg caggcgagtt ttcttgatgc gcttggtgcc ttcgcgcacc cattcccacg
     4081 ggtcctcctt gtgctcgcgc acatacttct tgaacataac gctcaagaag aactccatgg
     4141 attcgaagcc ggcctgatcc atgggctcgg cgatcggcag catggcgccg acgcgcatat
     4201 taagggccca gaggctttgg ttgccgtcac gcacagtcgt gtcgataaat cggatctcgc
     4261 tcatctgacc tccggaattt tagaatggtt caatccgttc aaatggttca aaacgttcaa
     4321 gccgttcggc tgccttgttt cggatctcca ggactactcg tggtgctggg tgtacctagc
     4381 cttccatagt ttgtataaat tcttgagggc tagatcaaac cgatcgttgg gagtgaattc
     4441 atgagaagct atcgaaagga actgtggttc aatacgccga cccgcgtcgc gttcgttaat
     4501 atcacggggc aagtgcaggg atgcattcga gaaagcggtg ttaacgacgg tttggtcctg
     4561 gtgaacgcca tgcatattac cgcttcggtt ttcatcaacg atgacgaatc cggcctgcac
     4621 catgactatg gtgtctggct ggaaaaattg gcgccgcacg aaccgctcaa gcagtatcgt
     4681 cataacgata ccggcgaaga taatgccgac gcgcacatca agcggcaggt gatggggcgg
     4741 gaagcggttg tcgccatcac cgacggtcgc ttggatttcg gtccctggga gcagatcttc
     4801 tatggcgagt tcgatggcaa acggcgcaaa cgggtgctgg tcaaaattat cggtgagtag
     4861 gagaagcttc tgaatcatga aatagctgcg cttcgatatt ttcgccgata tcgaatcgca
     4921 tcaggtcttc tccgacaagc agcggaccgg aataccgctt gcgcgtggcc gggatcaaat
     4981 cttcggcggt cgcgccgccg aagagcaaga gatggttgta aacggccagt ttcggtttga
     5041 cgcgggcaaa gacctcaccg gcttgctccg gagtggtatg attgctgccg attcgtttca
     5101 attgctcggg actctcggcg gcagtgccgc taacggcagt gatttcgtga accaccaggt
     5161 cggcgccgcg cgcatgattg atcaagtttt cattgaaagt cgtgtctcct gagagcacag
     5221 cggaacgtcc tcgataatcg attcgatagc cgaaggccgg caactcttcg ctgccatgat
     5281 cgacctcgaa tgccgtgatg attatcccgt tctgcgaata gacaacgcct tctttaatct
     5341 cctctgattc gagtttggcg ccggccatcg gatagcttct gctgcgaacg cgcagatcga
     5401 cggcgaatgc caacggtaaa tgttccatca tctgattcgt gccgtgaggt ccccagacat
     5461 ggagcggagt tttacggctt ccccaaggac ggccgatcca gccggtcagc cagagatcgg
     5521 cgaaaccaac caggtgatcg gaatgatgat gtgtgagcaa caaaccggtg atatcggcaa
     5581 acgggatgcc gagctgatgc agccgctgca tggcgccgcg cccggcatcg aagataaatt
     5641 tttgatctcc ggcttcgatc aaggtgctcg gcccgaagcg gcccaacagc ggaggcggcg
     5701 ctccggttcc cagcaaggtc acccggaaca aatctgcaac aggttctatc atgagtcctc
     5761 gcaaggatga tttcgccgcg ggaaacggca atgccagtca atctatcatc gctagaagaa
     5821 tttgtgcaat tgacccgggt gagcctggcc ggtatcgttg gatcagtgaa aaataacttt
     5881 aatagcggtt caccaccggc cagccggacg agatcagagc gccgttcgaa atcattcaag
     5941 atgcggcacg agatgcgctt ggagccggcg cagccgcgca ggaatcagac gtcggcggac
     6001 ggagcctata aatctccgtg cccgcactat ccctactgca tcggttgtcc gttgagtcat
     6061 gttccttacc cggagcagct gcttaagaag cgccatatcg tcgccggtgc gctcgcccgc
     6121 tatccgtcgc tgttggacgc tgaagtgcca cccgtggcgg cagcgccgca ccggctgggc
     6181 tatcgagcgc gcataaaact agttgtgcgc agcacccgcg gcgaaatcgc caccgggctc
     6241 tacgtgccgg gcagccatcg tgtgatcgat atctcttcgt gcccggtaca tccccggccg
     6301 gtgaatcagg tgcttcaata tctcaagaag aagattctcg agctgggcat cgagccttac
     6361 gatgagagag acgacagcgg gcagctgcgc tatttggatt ttcgctacag cttcgctcag
     6421 cgcgagttga gtgtgacgtt agtgacccgg cacggggatt ttcctcaagg tggcgcactg
     6481 gcccgttcgc tgagccgcaa attttccttc atcaacgggg taatccagaa cgtcaacccg
     6541 cagcgaggta acgtgatctg gggagaccgt taccggatca tctccggtcg cgacactttg
     6601 ctcgagcagg tcggccctgt gactcttgga tttcccgccg gggtattttc acaggcaaac
     6661 ccatctacgg cgaaaatgct ttacgagacg ataaacgcaa tggcggcgtt aaacgcccgc
     6721 gaaacggtgc tcgatctgta ttgcggtgtg ggaccgatct cgctctatct cgccggcacc
     6781 gcccgtctgg tttggggagt cgatgacagc tcgctttcga tcgacaccgc caaacagaat
     6841 gcgcgcagaa atggcatttc gaattgccgt ttcttcgagg gcgacgtggc agaaaaagtc
     6901 gccgaagcca aaaaaacttt gggtcaaatt gatctaatag tgctcaatcc gccgcgcaag
     6961 ggggttcagc cggaggccat ggaagcgatg ctcgcggtga atgctcctaa attgatttac
     7021 gtgtcgtgca accccgacag cctggcgcgc gacctcgata aactgctgag gcgacattac
     7081 cgcgtgcggc ggctgcagcc attcgatatg ttcccgcaaa ccgagcaagt ggaaacggtg
     7141 gtgtcgctgg agaaaagtta agtggtggtt gcaaaatctt agttcgttgt gattctattg
     7201 ctcaatcgac ggtcgggagg tgattatggc tgaattgaag gttgacggcg tggtccactg
     7261 gtcgattccg gtgaataatt tagaggaggc cgagaaattt tatggcgaga ttctcggttt
     7321 ggaacccaag ggacgtttgg gcgggtcggg gatgtcctgc tttaaagccg gggatcacaa
     7381 catattgcta tgcgtccgca acgagacaac cgtacgaacg ccgcaacagg acaatcggct
     7441 ccatcacgcc tttatggtga gtcccgaaat gtgggagaga ggatgccggc tgtttcagaa
     7501 aaatggcgtc aagatcgccg agccgatcgt gtatcgggag aagggtcact tcaccggtcg
     7561 cgaattatac ttcctcgatc cgagcggcaa tatgttggag ctgtgcgatc cgacctggaa
     7621 gcccggcatg cccaccccga gctacgaaga aatagtcggg acgaactaaa tatcttccca
     7681 tagcgcaaat tggcgccgcc tcttgccctt tcttccggcg gcgccaccag ctccccctac
     7741 gagagaaccg ccaacggaag acggcgtctc caacatgcat cgacgcgagc gattatatga
     7801 gcgcttccgg ataacgcgac ttgagcacaa gcgcaggggg ttacggcttg aacgccttga
     7861 acggcggcag ccaattggat tgaactgttg aacggtttag cgggcgccat tttcctgcga
     7921 gctgatcatg atctgcttta gtccggcagt gtcgtcgagt tgcccaccag ggctcatgtg
     7981 caaggagaat gcactcgtcg aggagaaaac catgctatgc tagacgattt cctgcagatt
     8041 cttccagagg tgattttcga ggccgccgag aagttaggcg gccgttgcac cggccgcttc
     8101 tacgcgctca atgcgctgga aaatcgtgtg tatgacatcg agatggaaga aggcccgcac
     8161 gtggtcgtca aattctaccg gcccggccgc tggagtcgcg ccatgattca ggccgagcac
     8221 gattttttga aggccttaga cgaaagtgag attccggtcg tccgcccgct cacggacgct
     8281 aacgggcaaa ccattaatga aactaatcaa gtgatgttca caatttttcc gaagcgtccg
     8341 ggacgactgg agcccgaact gacgacggaa cagctcatgc gcttaggaaa attcttggct
     8401 cgtcttcata acgttggggc gacggtaaaa gccgcgagcc ggttacggct tgatcctcaa
     8461 acctatgggc gagacccttt gaaattttta aaagacggac acttcgttcc gatggaactc
     8521 gcgagtcgat tcgaaaccat cgccacgcag atttgcgacg caatcactcc tcgcttcgag
     8581 ggagtcgagt acgttctgct gcacggagat tgccactccg gtaacattct atggaataga
     8641 gacgatccgt acttcatcga tttcgatgac atgctctatg cgcctcccgt tcaagacatc
     8701 tggatgctca cgggcgcaga cgacgactac gggagagagc agaggcgtat cctgctccag
     8761 gcatacgaac agatgcgaac tttcgacatc agaacgatgc atctcatcga gccgttgcgg
     8821 tcgcttcgca tgattcattt tgccgcgtgg atcgcgcgcc ggtgggaaga cagcgctttc
     8881 aaagcggcat ttcccgactt tggtaccgag cactattggc aagatcaaat cgaagcgctc
     8941 gcgctgcagt tagaaaagtg cgagaaccac acgagcgata tctaccgttg aacgtattcg
     9001 ttctatctat caatggccag tgaacacacg catcacttcg cctcgctgat ttcgaatcct
     9061 aagctgcgta gcaacgtgac tgtttcttct gactgcctac tgatttgacg caccaccgac
     9121 tgcagcgcgt cgaccgcatc gaccgcaaat cgcctgggcc aagctgcgat cgctgcggga
     9181 cggagcggta atcgtgcgcg aaaatcttaa cagtgaatcc aaaagtaccc gtgcgcggcg
     9241 aacaaaacaa aaattaagaa agtctagaaa taaaagtctg accgttgctt ctgcgaagcg
     9301 gccccacgat cttatgcctc gcttaaagag tcggttatgg gcatcgagcg atccgcaacg
     9361 cggtcgtctc acgatgtcgg aactgatttt ttatcatgtc gatttatgat aacgctcgaa
     9421 caatactatg gcgtttagga aaaatctgct cgctctgagc ttcgttcttg tctggattgg
     9481 ttgcgcgtcg gtcaccgtcg ccggcgcaca gctcgacagc gttactatcg ggttttcttc
     9541 cttttcagga ttttacggtc cgttgtggct cgccgtcgaa gatggtttgg gtaaaaagta
     9601 cggagtggac ctgagagccg tctacgcagg cagagtccgt ccccagcaat tgctcgccag
     9661 cggggagact cccttggtaa ttgcgtcggg aagcggcgcc atgacttcac acgttctcgg
     9721 cgtgaaggac caggtgatcg tggcgctgct ttccagcaaa atgggcggcg gcatattctc
     9781 caaaagcgaa attaaaagac ccgaagacct gaagggcaag ctgattgcaa ccggacgccc
     9841 aggcgctctt aacgacgttc tggttcgtta tgtgttgacg cgcaaatggg gactctctcc
     9901 cgatcgagat gtaaagctga tgccgattgg agagccgccg ctcatgctac aagctttgga
     9961 acggggcgtt gtcgatgcaa catcgttgtc ggttccggcg agctttctgg ctaagagcaa
    10021 gggatttttt gagctggtca attatgatgc cctggggctc agctaccccc agcatgccgt
    10081 aaccaccct
//
LOCUS       DASUVS010000022         3056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_443492, whole genome shotgun sequence.
ACCESSION   DASUVS010000022 DASUVS010000000
VERSION     DASUVS010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443492"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..283)
                     /locus_tag="VF182_01195"
     CDS             complement(119..283)
                     /locus_tag="VF182_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009724009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435714.1"
                     /translation="MATFKRLSVSVFAGILLLGGLAACDRKGPMERAGEKVDRSVERA
                     GDKVERATDK"
     gene            808..1725
                     /locus_tag="VF182_01200"
     CDS             808..1725
                     /locus_tag="VF182_01200"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435715.1"
                     /translation="MTFLFLAFIALKAPAAERFYISIPGPTLSYVPLYYGQEKGFFSR
                     DGLDLQILVVRGIVGVSSLMSGEIDVTCHAGSGFSAALRGLPLKIISVTRDRPIHELV
                     VGSFASPTELKGKSIAVGSLEGTAAIMTRRILQAKGLDPQKDVTLLSMDTPARLQSLL
                     TGKVAGAMMTPPSTYLAVDQGFKVFGRGRDYMRYLQTGVVATDTHIKQKRERLVRFLS
                     AWNRALEFYQENPELMIPYIQKRLGVKDASMAKRMYDDDAPFIISNGTLSGDAVREIV
                     DIGREALRIKEPIATEKIFDFTLAGEAAR"
     gene            complement(1883..2929)
                     /locus_tag="VF182_01205"
     CDS             complement(1883..2929)
                     /locus_tag="VF182_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6435716.1"
                     /translation="MKILTPALLFVFLINSPAWTQSVRPSSPAEIAGFSGSERERLLF
                     DGAKREGKLVWYTTLAAVQNKQIAATFEGKYPGVKVEIYRAGSSALAQRLLNEAQARR
                     HIADAIETTLPGLLTFRDNQLLLPYTSPYLTAFPEESREKGPANLVYWIIDRESYIGF
                     AYNKNLLSAGEVPQNFSGLLKPELRDKLGLAADDTGARSIGAMIKVKGEEFVRKLKEQ
                     NIKAYPMAGSALTQLVASGEVPASPSQFYSATHVAAKKGAPVAWVPMDINVVGAGGAA
                     VYANSQRPHAALLFVDFLLSPEGQKLLEDLDFGSARKDYGFKRWYPEKEGTLAQYEQA
                     LDRWHKLVREITFK"
BASE COUNT          706 a          840 c          793 g          717 t
ORIGIN      
        1 aatacaggga agaagtgaga gatcagcagc tactggaatg gcgaaagcgt cggggacgat
       61 caggcttagg agtcgatgcg gcgcgaattc gcgccgcatc tacggcctat tcatctggtt
      121 acttatccgt ggctctctcc actttgtctc ctgctcgttc aaccgatcta tcgacttttt
      181 caccggctcg ttccatcggc cccttccgat cgcaagcggc taatccgcca agcaatagga
      241 ttcccgcgaa caccgatact gataatcttt taaaagttgc catgagatgt cctcctctag
      301 atgtttatgg cgccacaagc cgcctcactg cggcgagaag caaccacccg tgctacatcc
      361 gcagcttcca tgccacaccg taaaataaag cgatatcaaa acctagcaag gcattattcg
      421 tttcgattgt ggggcatcgt caaaaattga gacgccgagc tatcggccgg gaagcttctt
      481 tgtcccgaaa gtcagacaaa ggggctcaga ggtgagacag ttaaggcatc gtgttctaga
      541 agctggctcc ggcaacaacg ggacatcaga ctagcgcggc aagactgttg aaccaagtaa
      601 ccggagcggt tcatctgttt agcttgtctt cgtttcctaa cagacttcaa cccgccaggt
      661 attacttcgg cagtatccgt gttatcgtcc gagccgttca taattgccaa acgggagaac
      721 acagacatgt atccaccaag tctgtcttgc ggcgaaagct cttcaccagc acggcttcgt
      781 catcccattt tgaggcgagt cgttgctttg acgtttcttt tccttgcgtt catagcgcta
      841 aaagcgccgg ccgcggagcg cttttatatt agcatccccg gtccgacttt gagctatgtt
      901 cctctctatt acggacaaga gaaaggattc ttctcgcggg acggcttgga cctgcagatc
      961 ctggtggtgc gcggtattgt cggcgtcagc agtctcatgt ccggcgagat tgacgtcacc
     1021 tgccatgcag gcagcggctt ttccgccgca ttgcgcggtt tgcccttaaa gattatatcc
     1081 gtgacccgcg atcggccgat tcatgaactt gtcgtcggct catttgcttc accgacagag
     1141 ctcaaaggaa aatcgatcgc cgtcggttcg ttagagggca ccgctgccat catgacaagg
     1201 cgcatcctgc aggccaaagg ccttgatcca caaaaggatg tcacgctgtt gagcatggat
     1261 acgccggcgc ggctgcaatc gttactgacc ggtaaagtcg cgggagcgat gatgactcct
     1321 ccatcgacct atcttgccgt ggaccaaggg ttcaaggtgt tcggccgcgg tcgcgactac
     1381 atgcgttacc tgcaaacggg cgtggtcgcc accgatacac acatcaagca gaaacgtgaa
     1441 cggctggtcc gctttctgag cgcgtggaac cgcgcgctag aattctacca ggaaaacccg
     1501 gagctcatga ttccctacat ccaaaaaagg ctcggcgtca aagacgcatc gatggccaag
     1561 agaatgtacg atgatgacgc gccatttatc atatccaatg gcaccctcag cggcgatgcc
     1621 gtcagggaga ttgtcgacat cggcagggaa gccttgcgaa taaaagaacc gatagccacg
     1681 gaaaaaattt tcgacttcac cttagccggc gaagcagcga ggtaggaggg tctgacaggc
     1741 cgatgacgag cggcgcgcga gatcttacaa gccggcttct tacgtgatct acggtggatt
     1801 gcgcaagccg gctgattatt cacctctata ctgaaaaagt tttgattgat cggcaagcaa
     1861 cgcagttgtg acccgcgtca cgctatttaa acgtgatttc cctgaccagt ttgtgccatc
     1921 ggtccaacgc ttgctcatac tgagcaagcg ttccctcttt ttccggatac cagcgtttaa
     1981 atccataatc cttgcgcgcg ctgccgaagt ccaagtcttc gagcagtttc tgcccttccg
     2041 gactcagcag aaaatccacg aaaaggagcg ccgcatgggg cctttgacta ttggcatata
     2101 ccgccgctcc tccagcgccg acgacattta tatccatggg gacccaggcc actggggcgc
     2161 ccttcttggc ggccacatgc gtcgcgctat aaaattgact gggcgatgca ggcacctccc
     2221 ctgaagccac cagctgggtc agagcgctgc cggccatagg atacgctttg atattttgtt
     2281 ccttcagttt cctgacaaac tcctcgcctt tgactttgat cattgcgcca atgctgcggg
     2341 cgccggtgtc atcggccgct aaaccgagtt tatcacggag ctcaggtttc agcagaccgg
     2401 agaaattctg cggcacctcg cctgcgctta gaaggttttt gttataggca aatccgatat
     2461 acgactcccg atcaatgatc caataaacca gattcgccgg ccctttctct cttgattcct
     2521 cgggaaaagc cgtcaggtat ggtgaagtgt aagggagtaa cagctggttg tcgcggaaag
     2581 tcaataatcc cggcagtgtc gtttctatgg catcggcgat atggcgccgg gcctgagctt
     2641 cattcaataa ccgttgggcc aacgcggaag atccagcgcg gtagatttca acttttacac
     2701 cgggatattt gccttcgaag gtagcggcga tttgcttgtt ttggaccgcg gccagagtcg
     2761 tgtaccagac cagtttgcct tcgcgtttag cgccatcgaa cagcagacgc tcacgctccg
     2821 atccgctaaa accagcaatc tcggccggag aagacggcct cacggactga gtccaggccg
     2881 gtgagtttat caggaaaacg aaaagtaagg cgggggtaag gatcttcaaa tcgaactccc
     2941 ttcattcgga aataggttct gtcttctccg acgtattgcc cgattactct ccagagtttt
     3001 tccgaggaca tttatcaatg ttcatacgcg gccggctgta aatgaatata agcgcc
//
LOCUS       DASUVS010000023         7263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_443579, whole genome shotgun sequence.
ACCESSION   DASUVS010000023 DASUVS010000000
VERSION     DASUVS010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443579"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1136
                     /locus_tag="VF182_01210"
     CDS             138..1136
                     /locus_tag="VF182_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435717.1"
                     /translation="MKAIGRSLIIVALIVLVSHSGASAQALKRIRIGYPSLSFRQSNV
                     WVATEMGLFKKYGLDVEPIFLRGGQTATLALVSGDPPIVNIGTVVQAGLQGHPLVLVA
                     AVETQYDQIVFVRPGITKLEELKGKNFGISGFGAATHYASTILVKHLKLESKDLPLVP
                     AGPDAERLAALLSGKVDATFFSSSAAPVARKAGLTELLQIADLGVEVQGNGFATSREY
                     IKTNRETVKSALKGFVEAIYFIYANKKDAQKVFAKYMRTDNPAVLEESYEGYINTIPK
                     KPYPTLKGIQFMLDMLAPQLPQAKNAKPEQFVDLSFLQELEKEGFFNEMAKRYPSK"
     gene            1184..1573
                     /locus_tag="VF182_01215"
     CDS             1184..1573
                     /locus_tag="VF182_01215"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6435718.1"
                     /translation="MATKTIQPESLNDPRPRYSQGIQAEGGRLLFIAGQTASDKNGNV
                     VGKGDIKAQTRQVFDNIKAVLQAAGGSLDNLVMTTTYITDRSYREGYNEVRRGLYKKD
                     PPTSTLLIVSGLANEDYMIEIAGIAVI"
     gene            1725..2726
                     /locus_tag="VF182_01220"
     CDS             1725..2726
                     /locus_tag="VF182_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEX6435719.1"
                     /translation="MRVTVLDDYQKALASTPAIKRLQQRAEVQILTEKLPDEGALVRA
                     LAGSRAVIPIRERTRFNAPVLNQLADLELISQTGNHVYHVDMEAATAAGIIVAMAPGG
                     NSTTEMAFGLMLAVMRRIPQSDQAMRRGEWPLVLGYVLKGKTLGILGLGKIGTEVAAI
                     GRAFGMNVIAWGPTLTAERAGKSQATYMGLEEVLKNADIVSIHLKLSEQSKNLLNEER
                     LRLMKKSAYLVNTARGAIIDELALVKVLREGAISGAALDVFVEEPLPPNHPLLQFSNV
                     VLTPHLGWPTDSGFEGFAENAVANILDYMDGKLTRVINPEALDRRASRSNPGRIIPL"
     gene            complement(2713..3969)
                     /locus_tag="VF182_01225"
     CDS             complement(2713..3969)
                     /locus_tag="VF182_01225"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX6435720.1"
                     /translation="MLTGAGLAMPGLFTQSSCAPLRIGSDRDTGLSLCYVAGDVTPDG
                     AIVWLRAEPGSEVMLHYSNDRQFSRFASAGPFRVDEKADYTVKIELQKLDPGTTYFYY
                     AAVTGKKPGYLASFLTAPAPADAAKVSFCFSGDSRESYQPFTIMNAVRAQRPNFFLHL
                     GDTIYADFGGTARKLSDFWAKYRANRNDPATQLCFFETSTYVVWDDHEVVNNFFAGNR
                     LVPTGRKAFFDYWPIRRHPSDEDRIYRSFRWGKALELFLLDTRQYRDPGTRTMLGTAQ
                     KDWLFKGLAASSAMFKFVATSVPMAGGGIDRWDGYPEERAELLRLIRDKRITGVVFLS
                     ADLHYAAVTSIPKSKGLKDITAGPLAAPLNRVTDGYASRFDFFLAENFNFAKITVDPA
                     VKPAQALVEFIDQDNAVFHTTRIKAV"
     gene            complement(4000..4506)
                     /locus_tag="VF182_01230"
     CDS             complement(4000..4506)
                     /locus_tag="VF182_01230"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX6435721.1"
                     /translation="MDTSTIVMLAAAILSLIAIYWKSPQAANDGLTATGALIIEITPR
                     MIAAFTLAGLIQAVVPQEVIVRWMGHGSGGKGILIGMALGSVTPGGPMTHFPVVASLY
                     KVGIGVGPLIAYLTAWSLFGLQRVIMWEIPFLGTKIVAVRFAVSFFFPVVAGWLCELI
                     WNKLQLQV"
     gene            4891..6174
                     /locus_tag="VF182_01235"
     CDS             4891..6174
                     /locus_tag="VF182_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6435722.1"
                     /translation="MTIDLEAANKVLAQIDRDELAQLGCDLTSIPSPTGQEKAIGEFI
                     LNWFHANGLKAVRQDVEFDRPNAVGIVRGDGTGLSLGFNGHTDTSFTGTAEDARMVAN
                     MEPESELKGKIVGNKVQGLGISNMKGGVAAFMMAGKALKKSQVKLKGDVILAAVVGEI
                     SRTPIGPWQTKEYRGEGAGTRHLLTHGMHSDYAVVADGSDLNIVWTQTGVVQVKIQTF
                     GKAEAAWGSNRSTHPMEKMNAIVKMTRIIEAVERWGAEFENKYVYNSPTGPLYPKVNI
                     GGIEGGAPYRPNYFPGVCTIYVDVRMPPQVRPVIIKYELETALNRLGLDYDMEIYKSL
                     LGHEGKNVEPLVKSAEEVYQHLFKEKIKPEAADRASIWTDTNVYNELGIPAIKIGPRG
                     RRIGPRNEEIEIDVMVKAAQIYALMALDICSRERA"
     gene            6480..6737
                     /locus_tag="VF182_01240"
     CDS             6480..6737
                     /locus_tag="VF182_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435723.1"
                     /translation="MAKSHDHNITLCLRYNLSLSGFNQQNAIQDSTSESEDPQNEDVR
                     WVGPAYRVNFQSSYGVGISFVCGSIEVDTVDRTCPEESVYA"
     gene            6805..>7263
                     /locus_tag="VF182_01245"
     CDS             6805..>7263
                     /locus_tag="VF182_01245"
                     /inference="COORDINATES: protein motif:HMM:NF042699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6602 domain-containing protein"
                     /protein_id="HEX6435724.1"
                     /translation="MTNSEHAFLSLPAYWDGIAEALSARLSAAKKYLRHPVAGFSAEG
                     YYIDLLREYVPKRYAVESGFVINAAGNRSHHLDIIVADTFHIPPLCSEPTYRVFAAES
                     VCAVIEVTTSPRGRDKGISKLEQDIAKLAQVRTLCQEREYFDVQPVLVGKK"
BASE COUNT         1804 a         2008 c         1986 g         1465 t
ORIGIN      
        1 acaaaggcaa aaaggtgccg tcttacaccg aatatgtcga caatacgttc gtggaagaag
       61 cgcagaagct ggctgctgtt aagtagcgcc tcgattcgac tttgaatgac tggcgcatga
      121 cggaaggaag ataggacatg aaagcaatcg ggcgatcttt gataatcgtt gctttgatcg
      181 tactcgttag ccatagcggc gcgagcgctc aagctctaaa gagaattcgt atcggctatc
      241 cgtcgttgag cttcaggcaa agcaacgtct gggtggccac agaaatgggc ttgttcaaga
      301 agtacggcct cgacgtcgag ccgatctttc tccggggcgg ccaaaccgcg accctggctt
      361 tggtttccgg cgatccgccg atcgtgaata tcggcacggt ggtgcaggca ggtctccaag
      421 gccatccgct ggtgttggtt gccgcggtcg aaacccagta cgaccagatc gttttcgtcc
      481 gacccggcat aaccaaactc gaagaactca aagggaaaaa cttcggtatc agcggatttg
      541 gcgcggccac gcattacgcc tcgaccattc tggttaagca cctgaagctc gagtccaagg
      601 atcttccgct ggtgccggcc ggacccgatg ccgagcgcct ggccgccctg ctttcgggaa
      661 aggtcgatgc cacctttttc agctcttcgg ccgcgcccgt ggcaagaaaa gccggattga
      721 ccgagctgct gcaaatcgcc gatctaggag tcgaagttca gggcaacgga ttcgcaacct
      781 cgcgcgagta catcaagact aatcgggaaa ctgtaaaatc ggcattgaaa ggtttcgtcg
      841 aagccattta ttttatttac gccaacaaga aagacgcgca gaaagttttc gccaagtata
      901 tgcgcaccga caatcccgcc gtgctcgaag agtcctacga aggctatatc aacacgattc
      961 cgaaaaagcc ttacccgact ctcaaaggaa ttcagttcat gctcgacatg cttgcgccgc
     1021 agctgccgca ggcgaaaaat gccaagcccg aacagttcgt cgatctgagt tttttgcagg
     1081 aactggaaaa agaagggttc ttcaacgaaa tggcgaagcg gtatccaagc aaatagcaag
     1141 cttccgaacg tctaaacaag tcgcgcacaa ggaggagtga tttatggcaa ctaaaaccat
     1201 ccagccggaa tctttgaacg atccgcggcc gcgttatagc cagggaatac aagcagaggg
     1261 cggaaggctg cttttcatcg ccggccaaac cgcttcggat aaaaacggca atgtcgtcgg
     1321 caagggggac atcaaagctc aaacccgcca ggtgttcgat aatatcaaag cagtcttaca
     1381 agccgcggga ggctcgctcg acaatctggt gatgaccacc acctatatta cggatcgaag
     1441 ctaccgggaa ggatataacg aagtccgccg cgggctctat aaaaaagacc cgccgaccag
     1501 cacgcttcta atcgtcagcg gcctggccaa cgaagactac atgatcgaga tcgccggaat
     1561 cgccgtcatc tagcagtttt atgaaaaact ctgaatgcag gctgctcaaa aagatctcga
     1621 aggcgagtcg cgcgaatcac aaggatgaaa gcgtaaatct gaaagcggaa ttcagtttac
     1681 agctttcata attcgtcttt tccgcagcct gccagtttaa ggctatgaga gttacagttc
     1741 tcgacgatta ccagaaggcg cttgcaagca cgccggcgat caaacgcctg cagcagcgcg
     1801 ccgaggtgca aatcctgact gagaaattac cggacgaggg cgccctagtt cgcgccctcg
     1861 ccggctcgcg cgcggtgatt ccgattcgcg agcgcactcg attcaacgcg cctgtgttaa
     1921 accaattggc ggatcttgaa ttgatctctc aaaccggcaa tcacgtttat cacgtcgaca
     1981 tggaagcggc gaccgcagcg ggcatcatcg tcgccatggc gccggggggc aacagcacga
     2041 ccgagatggc cttcggtttg atgttggcgg tgatgcgccg gattccgcaa agcgatcaag
     2101 cgatgagacg aggcgagtgg ccgctggtgc tcggctatgt tctaaagggc aagacactgg
     2161 gaattttagg tctaggaaag atcggcaccg aggtcgccgc gatcgggcgc gctttcggca
     2221 tgaacgtcat cgcctggggg ccgaccttga cggccgaacg cgccggcaaa tcacaggcga
     2281 cctacatggg cctggaagaa gtcctgaaaa acgccgatat cgtttcgatc cacctaaaac
     2341 tttcggagca aagtaagaac ctgctgaacg aagagcggct tcgcttgatg aaaaaatccg
     2401 cttatctggt caacaccgcg cggggcgcca tcatcgatga gctggcgcta gtgaaagtat
     2461 tacgcgaagg cgctatttct ggcgccgctc tcgacgtctt cgtcgaagaa ccgcttccgc
     2521 cgaaccaccc gctcctacaa tttagcaacg tcgtactaac tccgcatctc ggctggccaa
     2581 ccgattccgg ttttgaaggc ttcgctgaaa acgccgtcgc caacattctc gattacatgg
     2641 acgggaaatt gacgcgggtc atcaatcctg aagcgctcga tcgtcgcgcg tcacgcagta
     2701 accccggccg gatcataccg ctttgatcct ggtggtatga aacactgcat tatcttgatc
     2761 gataaattcg actaacgcct gggccggttt aaccgctgga tccacggtta tttttgcaaa
     2821 gttaaaattc tcggcgagaa aaaaatcgaa acgggacgca tagccgtcgg tcactcgatt
     2881 caagggcgcc gccaatgggc cggcggtaat atccttgaga cccttgcttt tgggaatgct
     2941 cgtcaccgct gcataatgaa gatcggcgct caggaaaacc acgccggtta tgcgcttatc
     3001 tctgattaac cgcagcagct ccgcccgttc ctccgggtag ccgtcccaac gatcaatccc
     3061 gccaccagcc ataggcacgg aagtcgccac gaacttaaac atcgcggaag aggccgccag
     3121 ccctttaaac agccaatcct tctgcgccgt ccctagcatg gtccgagttc ccggatcccg
     3181 gtactggcgc gtgtccaaca gaaataattc caaggccttg ccccaacgaa acgagcgata
     3241 gatccgatcc tcatcgctgg ggtggcggcg gatcggccag tagtcaaaga acgcctttct
     3301 cccggtcggc accagccgat tgccggcaaa aaaattattc accacttcgt gatcatccca
     3361 aacgacgtag gtgctggttt cgaagaaaca gagctgcgta gcgggatcgt tgcgattggc
     3421 gcggtacttc gcccagaaat cactcaactt ccgcgcggta ccgccgaaat cggcatagat
     3481 ggtgtcgccc agatgaagaa aaaaattagg tcgctgcgcg cgcaccgcgt tcatgatggt
     3541 aaacggttga tagctttccc gactatcacc gctgaaacaa aatgaaacct tggcagcatc
     3601 ggccggagcc ggcgcagtta gaaagctggc gagatatccc ggctttttac cggtgactgc
     3661 ggcgtaataa aagtaagtcg tcccgggatc gagcttctgc aattcgatct ttacagtata
     3721 gtcggccttc tcatccaccc ggaatgggcc ggcggacgcg aagcgactaa attgacgatc
     3781 gttactatag tgcagcatca cctcgctgcc cggctcggcg cgcagccaaa cgatcgcgcc
     3841 atccggtgtt acgtcgccag ccacatagca gagtgacaaa ccggtatcgc gatcactgcc
     3901 aatacggagg ggagcgcacg atgactgggt gaagagcccg ggcatggcca agcccgcgcc
     3961 ggtcagcatt cgaagaaatt ctctgcgcct caccttggat taaacctgga gctgaagttt
     4021 gttccagatc aactcgcaga gccacccggc aaccaccggg aaaaaaaagc tcacggcgaa
     4081 gcgcaccgcg acgatcttcg ttccgagaaa aggaatctcc cacatgatca cacgttgcag
     4141 gccgaagaga gaccaggcgg tgagataagc gatcagaggc ccgacgccaa tgcctacctt
     4201 atagagcgac gccaccacgg gaaaatgcgt catcggcccg cccggcgtga cgctccccag
     4261 ggccatgccg atcagaattc ctttgccgcc cgatccatgc cccatccacc gaacgatgac
     4321 ttcctgcggg accaccgcct gaatcaagcc agccagtgtg aacgcggcga tcatgcgcgg
     4381 agttatctcg atgatcaatg ctccggtcgc ggttagacca tcgtttgccg cttgcggcga
     4441 cttccagtag atagcgatca gcgacaggat cgcagccgcc agcatgacaa tggtggacgt
     4501 gtccatagat ctccttagac tcaggcggaa tttctctcgg gtgggcattg atgaagtttt
     4561 gaccggacgt aaagcgtagc ctgcaacttt aaacagcaag cgataatttt tcaagcagac
     4621 cgtttcgcaa cgagcatttg acttgccggc gtcactatag tctttatagt ttccaatagg
     4681 ctgctgaaaa agcctgatag gcttcgttgc acctcaaaac gcttcgccaa actcgtgttc
     4741 gtggtacgtg ttcgtgtccg ttctggaatc cgcgatcacg tttgcacgga cacgatcacg
     4801 aggatgcggt cctcgccgtg aatcttgtgg agcagcctat gggcagagtt cttagcagac
     4861 tgacagtaat tgaattagaa ggagaccgaa gtgaccattg atttagaagc ggcaaacaaa
     4921 gtgttagcgc aaatcgatcg cgatgagctg gcccagctgg gctgcgatct caccagcatt
     4981 cccagcccta ccggccagga aaaagccatc ggcgaattca ttttgaattg gtttcacgcc
     5041 aacggcctca aagccgtgcg acaagacgtc gaattcgatc ggcccaatgc cgtaggcatc
     5101 gtcagaggcg acggcacggg attaagcctg ggcttcaacg gccataccga taccagcttt
     5161 accggaacgg cggaagacgc gcgcatggtc gccaacatgg agccggaatc agagctgaaa
     5221 ggcaaaattg tcggcaacaa agtacagggc ctgggcatct ccaatatgaa aggcggcgtc
     5281 gcagcgttta tgatggccgg caaagcgctg aagaaaagtc aggtcaagct taaaggcgac
     5341 gtcatcctcg cggcagtggt cggcgaaatc tcgcgaactc ccattgggcc gtggcaaacc
     5401 aaagaatacc gcggcgaagg cgccggcacc aggcatctgc tgacccatgg catgcattcg
     5461 gactatgccg tagtcgccga tggctcggat ttaaacatcg tctggacgca aaccggtgtg
     5521 gtgcaagtga agattcaaac cttcggcaaa gccgaggccg catggggctc caaccgttcg
     5581 actcatccga tggaaaagat gaacgccatc gtcaagatga ccaggatcat cgaagcggtt
     5641 gaacgctggg gcgccgagtt cgagaacaaa tacgtctaca actcccctac cggcccgctg
     5701 tatcccaagg tcaatatcgg cggcattgaa ggcggcgcgc cgtaccggcc caactatttt
     5761 cccggcgtct gtacgatcta cgtcgatgtg cgcatgccgc cgcaagtcag accggtgatc
     5821 atcaagtatg aattggagac ggcgttgaac cgcctcgggc tggactacga catggagatt
     5881 tacaagtcgc tgctcggcca tgaaggcaag aatgtcgaac cgctggtgaa atcagccgaa
     5941 gaagtttatc agcatctgtt caaagaaaag atcaaacccg aggccgccga tcgcgccagc
     6001 atctggaccg acaccaacgt ctataacgag ctcggcattc ccgccatcaa gatcggtccg
     6061 cgcggccgcc gcatcggccc gagaaatgaa gaaatcgaaa tagatgtgat ggtgaaggct
     6121 gcgcaaatct atgcgctgat ggcactggat atttgcagcc gggaacgggc gtaggacgct
     6181 ttataccgct tcactttcgg cgaagatcag ctttggcggc ttatgggcgg cacatcgcac
     6241 tggcatagcg ttaggcgtga tccgaaagcc atgatggaat tcctcaaccg aaccgatgtg
     6301 gtaaaggatc acggaaaagc taactgagat cttcgggcgt tggccttcgt tccatgattc
     6361 agaggtcatc aggatccggt tggagcaccg tgggcgagat aagtgggagg ggccggtcgc
     6421 gtacgtcagc gtccatgtgt tcgagggtta tcgccaaaac gaggcgtcta ccgggatcaa
     6481 tggcgaaatc acacgatcat aacatcacgc tttgcctccg ttataacctg tctctgagtg
     6541 gcttcaatca gcagaacgcg atacaggatt ccacgtccga gtccgaagac ccgcaaaacg
     6601 aagacgtgag gtgggtgggt ccggcgtacc gagttaactt tcaatcatcc tatggcgtag
     6661 gcatttcgtt tgtctgtggc tctatcgaag tcgacacggt ggaccgaacc tgtcccgaag
     6721 agagcgttta cgcctaacaa ttgggttgag atggccgcgg cggacggtgc cgctcaccca
     6781 aacgttagac gccgagacac atcgatgaca aattccgaac acgcatttct ctcgttgccg
     6841 gcctactggg acggcattgc agaggcattg agtgcccggc tttcagccgc caagaaatat
     6901 ctacgtcacc ctgttgcggg atttagcgct gagggttact acattgactt acttcgtgaa
     6961 tatgtcccga agcgctacgc tgtggaatct ggcttcgtca tcaacgccgc tggtaacaga
     7021 agccaccatc tcgacatcat cgtcgccgac acgtttcaca ttccccccct ttgctcagaa
     7081 ccgacgtacc gtgtatttgc cgctgaatct gtgtgcgcgg tcatcgaggt cacaacatct
     7141 ccacgcggcc gcgataaagg catcagcaaa ctagagcaag atatcgctaa gttggcgcag
     7201 gtccgaactt tgtgccagga gcgcgagtac ttcgatgttc agccagtcct ggtcggaaag
     7261 aaa
//
LOCUS       DASUVS010000024        14967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1317199, whole genome shotgun sequence.
ACCESSION   DASUVS010000024 DASUVS010000000
VERSION     DASUVS010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14967
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317199"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..357)
                     /locus_tag="VF182_01250"
     CDS             complement(160..357)
                     /locus_tag="VF182_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX6435725.1"
                     /translation="MFGLGMQELLIILAIALVIFGPSKLPQIGSGLGKAIRDFKKGVS
                     SDEDEDSIKQAKDENSKDLHR"
     gene            complement(366..1802)
                     /locus_tag="VF182_01255"
     CDS             complement(366..1802)
                     /locus_tag="VF182_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX6435726.1"
                     /translation="MLPFQFYFQLGLGLAMAYFLPSIAAHGDWSSSAAAASLSSLESR
                     PPSFAAIAKRTMPIVVNISTTAQRAPRGTSGDPIDEFFNRFFGDSQPRDNSQRSLGSG
                     ILISKDGEILTSYHVVKNADAIKVRLADQSEFDARLIGKDDRTDLALIKIRKTNVPLP
                     FARLGTSSRLDVGDWVMAIGNPFGLEHTVTAGIVSAKGRVIGAGPYDNFIQTDASINP
                     GNSGGPLINAVGEVVGVNSAIFSQSGGNVGIGFAIPIDLAKKITDQLRKNGKVVRGWL
                     GIRAQAVSQQAASSSGLSHFAGEIQTVTEVTENSPAAEAGIRVGDVIIEFNGKPLSKN
                     PDLRTMIADTAPGHKITLKIVREKLERVASVRIGELPDDGDGSQQVEARDPELGLRVQ
                     RITPESSRRLGLSSIKGVLVLEVQSGSPAEQVGLEPADVIREVNQKPVNNVKDFERAV
                     RQGRRGERILLLVQRGDNAVFFALKRKS"
     gene            1946..3298
                     /gene="mgtE"
                     /locus_tag="VF182_01260"
     CDS             1946..3298
                     /gene="mgtE"
                     /locus_tag="VF182_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX6435727.1"
                     /translation="MKSTPIDYDTVREMLRTATPDRILRFIGKSHPADIALLFKGLDP
                     AEVRQFFDILFSARRAAKTLTELPPELLPDILALIDDEKIARVIVRADPDDAVTIIDS
                     LTEERRESVLSLLDPERREEVRELISYPEGTVGRIMNTDFMALAPTITAQGAIDKIRE
                     RGELASFFYLYVVDQENKLVGVVPIRNLVIAPRDRTLSEMMIADPLKGDVFMDQEEAA
                     RIVAKYELLALPIVDEGGRLEGIITVDDVIDIINEESTEDMYKMAGLAEEDRVFTPIS
                     RSVKMRLPWTMLNLLTTGLAASVVTIFQGTITELVALVTFMPIVAGVGGNGATQTATV
                     IIRGIALGELEFSSAWKAIVKQVSVNVCLAVAAGAVIALIAALWQGNPVLGFVLALAM
                     ILNLGLVAGFSGAVIPLLLKAMKFDPALGSGIVVTGLTDAFGFLSFLGLATLFIHYLT
                     "
     gene            complement(3295..4269)
                     /locus_tag="VF182_01265"
     CDS             complement(3295..4269)
                     /locus_tag="VF182_01265"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX6435728.1"
                     /translation="MKNRNRIVIFILKLVVSVGLLGYIITRIDGEQFAHTLASANFSY
                     IALALVIYLGTQVVSALRWTVLARPLGIRTPYKDLTAYYFIGMFFNLFAPSTVGGDVS
                     RVYYLAREENRDQHKRASGSTIQAAVSVLMDRGIGMVVLIWLGALGLLLFPEYEVPRA
                     IRALTFSLSVAFLIGGLLLPLVRRVLPEDAHPLITKLRVILTSYRKGWRVIPQAITLS
                     FMVHLVQAWMHVIMGLALHLSLPLSFCIIVYPLVGTFSALPISVNGFGLREGGYIFLL
                     ALIGIDSEEGIAFGLLLFLIVAVDSLIGGILFLVKKDSVAAGAVSAEV"
     gene            complement(4266..4928)
                     /gene="purN"
                     /locus_tag="VF182_01270"
     CDS             complement(4266..4928)
                     /gene="purN"
                     /locus_tag="VF182_01270"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX6435729.1"
                     /translation="MVRQLSIGVLISGNGTNLQAIIDAIEARALDAKIAVVLSNKADA
                     YGLARAQKHSLYTAVLDHKSFFSREAYDQAIVDILRRCGVELVVLAGFMRLLSPPFIK
                     AYSNRIMNIHPALLPAFPGLRVQKKALEHGVRFSGCTVHFVNDECDEGPIIMQAVVPV
                     FQDDSETALAARILKQEHRIYPRAIQLYAQGKLHISGRRVMVDDVPKDEKQVLIQPAC
                     EE"
     gene            complement(4939..5961)
                     /gene="purM"
                     /locus_tag="VF182_01275"
     CDS             complement(4939..5961)
                     /gene="purM"
                     /locus_tag="VF182_01275"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX6435730.1"
                     /translation="MTYAAAGVSIEAGDELVRRIGRVAKKTKLPGLLAGVGGFSALFD
                     LKARRYHQPVLVSSTDGVGTKLKLAFATGIHETVGIDLVAMSVNDILTQGAEPLFFLD
                     YFVCGKLDVVIAEAVIRGIAQGCKQSGCILIGGETAEHPGDFPDGEYDLAGFVVGALE
                     KKKILNPKAIVPGDLLIGLRSSGLHSNGYSLARKILLSHGGLKLGQRLPELGRTLGEE
                     LLAPTRIYAKTMRNLLAKSSIKGAAHITGGGIVGNLPRVLPQGVRALIHRDSWPQPPI
                     FGMIAKIGAVSRDEMDRTFNNGLGMILVVGKKAADGAMRSLRQSGEKPMVIGEIRKGP
                     RGADII"
     gene            complement(6056..6850)
                     /locus_tag="VF182_01280"
     CDS             complement(6056..6850)
                     /locus_tag="VF182_01280"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6435731.1"
                     /translation="MPPLIAVDNASRVFTSGAKIVTALERISFEIRAGEFVSIVGPSG
                     CGKSTLLKILSGLLPASSGKILISGRSVDQPLENVGMVFQAPVLLKWRPVLGNILLPV
                     EFAKRNVASHMERARSLINLVGLQGFEEMYPHQLSGGMQQRVSLCRALVTDPQLLLMD
                     EPFGALDAMTRDELDLELLRLWEEKKKTVLFVTHSIQEAVFLSDRVFVMSARPGRLVE
                     VLSIDLTRPRTLEMMSSVHFGEYTLRIRSLLASAMGTPEAIAGSTA"
     gene            complement(6939..7961)
                     /locus_tag="VF182_01285"
     CDS             complement(6939..7961)
                     /locus_tag="VF182_01285"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435732.1"
                     /translation="MTRVDIEFSIHRVAAAVLSALMAMITFSFNAVAAQPTPANIRMD
                     FIIGGKHAPWYVALEKGFYAKRGLAATVQAGSGSADTVRTIAAGGADFGFADISTAIV
                     ARSRGTGVQVVGQLGYVAATILWREDSAIKGIKDLQGKSWAISAGQAQWFLMPAYCRI
                     NGIDFKSIKIQETAAPIQPAALATKKSDFIIMFRASNDEVAELAAAKVGTKLNRVFMK
                     DTGLDIYGSGLVAKEEDIKKRPDFVRAYVEGTMEGLRYTRDNQEEALKILLKQKPELD
                     QALTTLQLRNALNEVMLPAESVQSGLGYMKPDIMDKTVRITNEYFDVARKVTAKEVFT
                     NQFIKR"
     gene            complement(7979..8755)
                     /locus_tag="VF182_01290"
     CDS             complement(7979..8755)
                     /locus_tag="VF182_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6435733.1"
                     /translation="MKASTRNIIEPIVFFLALLILWEILVEIIQVPSFILPPPRDLWI
                     SFIQKLPVIGHHAWITFIEAFGGFALSLLLGIAFAVAVVYSPHLQNTIYPLIVILYAM
                     PKSAFAPLMVIWVGYGLMSKIAIAFLVAFFPIVVNTVVGLKEVEPELLELARINRGSQ
                     FDVFKKIRLPNSLPYLFAGIKVAIVLSVTGAIVAEFVAANEGLGYLVLQANYSLDTAF
                     ALVILLILAVLSLGLFWLVEIIQRKLAPWSAEVRQVEGSF"
     gene            complement(8976..10136)
                     /locus_tag="VF182_01295"
     CDS             complement(8976..10136)
                     /locus_tag="VF182_01295"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6435734.1"
                     /translation="MSVDVSIRAVRKISEISQVQWDALIANGSPFLKWHWLDSLEQSG
                     CVNDDTGWLPHHLVVERAGKVVAACPMYLKLHSMGEFVFDYEWAETAHHVGIQYYPKM
                     LVGVPFTPVTGNRFLTGAGTDRSALITIMGQALAKIAVDNKISSVHVNFCSDDEVAAL
                     EQIGFIARRGIQFHWQNHKFHSFDDYLGSFRSDRRNKIKRERREVAQRGISIRAIEGE
                     ELTHKHMRTMFRLYKGHIDRLYYGRQYLTQEFFDQLHLRFASNICLMLAEGDGKIIAG
                     TFNIRDDTAMYGRYWGAFQDQPFLHFNVCYYSAIEHCITMGLQRFEAGAGGSFKQLRG
                     LDPEHTTSMHYIVDGRFRRAVERFLKQERELIRQKRDVLLERSQLKRELEEP"
     gene            10285..10728
                     /locus_tag="VF182_01300"
     CDS             10285..10728
                     /locus_tag="VF182_01300"
                     /inference="COORDINATES: protein motif:HMM:NF018391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEX6435735.1"
                     /translation="MVIYNLLCKKKHGFEAWFPSFEEYQKQAEQKLISCPTCGTTKVE
                     KVPHACAVHVKKEQPAPPAKTAAQTPPAPPSPAEFKEMLLRVHHYVKQNFEDVGPRFA
                     QEARQIHQGKAEERPIYGTATVTEAAELADEDIPHVVLPKPDLDS"
     gene            11015..12097
                     /locus_tag="VF182_01305"
     CDS             11015..12097
                     /locus_tag="VF182_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6435736.1"
                     /translation="MLLINNQTVEQILDMKGCMAALETGYRDLIDERAVYRGRYDLFV
                     PNDDPKLMYRWGTMEGASRSFETFAIRMKSDMLEWPEGKTVEKYCIEPGTFCGIVMVF
                     STRNGEPLAIINDGVIQHMRVGGCAGLGVKYLSREDSKVVGIFGAGGMARTYLLAFNE
                     VRKIAEVRVYSPTKKNRDIFAQEMGEKLSIKIIPVERPENAVRGCDIVATCTDSIQVV
                     VDDPSWIEPGMHLTCVKSNEWNPEIVKNSDLVIKMGRATINLDVGMRRIGGEAAVVAG
                     NEEEIKRIANPRVDIFSGDFPVLTDVMSGKLKGRTDRNQVTFFANSGTQGLQFASTAG
                     YVVREAKRRGLGQEIPTQWFLQDIRD"
     gene            complement(12559..13788)
                     /locus_tag="VF182_01310"
     CDS             complement(12559..13788)
                     /locus_tag="VF182_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435737.1"
                     /translation="MKAAVSDDQTVVAREAQRAEHEIEGLKANIAGLEAKLRETEDIV
                     HRKEAASQKMEENLNAEIRDLQNVVRTKEEALERRESEINVLNSKIDVMAGQVSHLEL
                     AIEHARGEAASKNQQAEQVIDGLKTNISALEARLRETEDTIHKKDIASRKMEDSLAAE
                     IRDLESLLKTKEEALQGRQSEVNDLKSKMDALVDQVTHLELTNGHAKETAASKAHQAE
                     QVIEDLKMKIVTLQAQLSQTEQIVAGLVANGRVPDTESTIKQSCEDRNQDIDLDVHVE
                     PHTNGIKHDTAAALIDIQAPQTSGTAGREPMETDVERSTSFEFQAIGATSTITEAASQ
                     TVSPYAFDRMIAEFSELSNVMRSIASLIVRDHVRAFGESMEEFPQERFPELVASLSKT
                     ISEEKLKTDFCQRFSKM"
     gene            complement(14397..>14967)
                     /locus_tag="VF182_01315"
     CDS             complement(14397..>14967)
                     /locus_tag="VF182_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6435738.1"
                     /translation="ERGGLGLGLTLVRSLVELHGGNVEARSEGLGRGSEFVVRIPIGE
                     GDPVVEDQDGAVRPNAQTVGEKGRRILVVDDLAIQAKSLAILLQLRGHDVRIAHNGAD
                     ALEILHEFPAEVALIDIGLPGMDGYGLAGRIREQPQFSKTVLIAQTGWAMENDRLRSR
                     EAGFDYHLAKPIDHGRLHEILTESPQTDT"
BASE COUNT         3512 a         4175 c         3845 g         3435 t
ORIGIN      
        1 aacggccaac attttggtaa tggcgcccca gccaaaaaca cacaccactg agtcaaccag
       61 agctgtattc tgcatacccc ttgattactc aagcgtagcc cggcattccc gaatcgccaa
      121 aacaatcgac aggggagaaa cctggagtgc cggaatttgc taacgatgaa gatccttgga
      181 gttttcgtcc ttagcttgct tgatggaatc ctcgtcctcg tcgctgctca ctcctttttt
      241 gaagtcgcga atggctttgc ccaagccact gccgatctgc ggcagcttgc tgggaccaaa
      301 aatgaccagc gcgattgcca agatgattaa aagctcctgc attccaagtc cgaacataaa
      361 ccctcctagc tcttacgctt caaagcgaag aatacagcgt tatctcctcg ttgaacgaga
      421 agcaagattc gctccccacg tctgccctga cgcacggcgc gttcgaagtc tttgacattg
      481 ttgaccggct tttggttaac ctcacgaatc acatccgcag gctctaagcc tacctgttcc
      541 gcagggctgc cggattgcac ttccagaacc aaaaccccct ttatggagct cagtcccaga
      601 cggcgcgaac tttccggagt gatgcgttga acccgtaatc ccaactccgg gtcacgcgct
      661 tccacctgct ggctgccatc accatcatcc ggcaactcgc caatgcggac ggaggcgacc
      721 cgctcgagtt tctcgcgaac aatcttcaac gttatcttgt gcccgggcgc ggtgtcggcg
      781 atcattgtgc gaagatcggg atttttcgaa agcggctttc cgttgaactc aataattaca
      841 tcaccgaccc tgatgcccgc ttctgccgct gggctatttt ccgtaacctc ggtaactgtt
      901 tgtatctctc cggcaaaatg agacagaccc gatgaagatg ctgcttgctg tgaaacggct
      961 tgcgcgcgaa tcccgagcca gccacgcacc actttgccgt tctttcggag ttgatcggtg
     1021 atctttttgg ccagatcgat gggaatggca aagccgatgc cgacgttgcc gccactctga
     1081 ctaaaaatag cgctgttcac tcccacaact tcaccgacag cattgatcag cgggccaccg
     1141 ctattgccgg gattgatcga tgcatcggtc tgaatgaaat tatcataagg tccagcgccg
     1201 atgacccgtc ccttggcgct gacaattccc gccgtcaccg tatgctccaa gccgaacgga
     1261 ttaccaatcg ccatgaccca atcacccaca tccaatcggc tcgaggtgcc gagccgagcg
     1321 aacggaagag gaacattggt cttgcgaatc ttgatcaacg ctaaatccgt tctgtcgtcc
     1381 ttgccaatga ggcgtgcgtc gaattcagat tggtcggcaa gccttacttt aatggcgtcg
     1441 gcgttcttga cgacgtgata gctggtcaaa atctcgccat ccttactgat cagaataccg
     1501 gacccgaggc tcctctggct gttgtctcga ggctgggagt cgccaaagaa acgattgaag
     1561 aactcatcaa tcggatcgcc ggaagtgccg cgcggagcgc gttgtgctgt cgtcgagatg
     1621 ttgacgacga tgggcatggt ccgtttggcg atggcggcaa acgatggcgg gcgactttca
     1681 agcgatgaca aactagccgc agcagcgcta gacgaccaat cgccgtgcgc ggcgattgag
     1741 ggcaggaaat atgccatagc caggcccaaa cctagctgga aatagaattg gaacggcaac
     1801 atgcttttct cgcttgcgcg gccaaagtga aactcacgat agcagatttt tatcctccct
     1861 ataagggttc caattggtta gaatcatttg caaatcgata taccaatggt taagaagaga
     1921 cgcaaagtcg cgatccaaga acaagatgaa atccactccg atcgattacg acacggttcg
     1981 agagatgttg cgcactgcga cgccggatcg catcttgcgc ttcatcggca aaagccaccc
     2041 cgccgacata gcgctgctct tcaaggggct ggaccccgcc gaggtgcggc aatttttcga
     2101 catccttttt tccgcccggc gagccgccaa aactctcacc gaactaccgc ctgaattatt
     2161 gcccgacatc ctggcactga tcgatgatga gaagatcgcc cgcgtgatcg tgcgcgccga
     2221 tcccgatgat gcggtgacaa ttatcgacag cctaacggaa gagcgccgtg aatctgtctt
     2281 gtcgttactg gaccccgagc gccgcgagga agtccgcgag ctcatcagct atccggaagg
     2341 caccgtcggc agaatcatga acacggattt tatggctctg gcgccaacca ttacggctca
     2401 aggcgccatt gataaaatcc gtgaacgcgg cgagcttgcg agtttttttt acctctacgt
     2461 ggtcgatcag gagaacaagt tagtcggtgt cgtgccgatt cgcaatttag tcatagctcc
     2521 ccgcgatcgc acactgagtg agatgatgat cgccgatccg ttaaaaggcg acgtcttcat
     2581 ggaccaggaa gaagcggcgc gaattgtggc gaagtacgag cttctggcct tgccgatcgt
     2641 cgatgaaggc ggtcgattgg aaggcatcat caccgtcgat gacgtcatcg atatcatcaa
     2701 cgaggaaagc accgaagaca tgtacaagat ggccggttta gccgaggagg accgagtttt
     2761 cacgccgatc tcccggtcgg ttaagatgcg cctgccctgg acgatgctga atttgctcac
     2821 aactgggctg gcagcatcag tggtgacgat ctttcaaggc acaatcacgg aacttgtagc
     2881 cctggtgacg tttatgccga tcgtcgccgg cgtcggcggc aacggtgcaa cccaaactgc
     2941 gacagtgatt atccgtggca ttgcgctcgg cgagcttgag ttctcgtccg cctggaaagc
     3001 gatcgtcaaa caggtttcgg tcaatgtttg tctggctgtt gcggccggcg ccgtgatcgc
     3061 tctgatcgcg gctttgtggc agggaaatcc agttctggga ttcgtgcttg ctttggcgat
     3121 gatactgaat ttgggattgg tcgccgggtt ctcgggagcg gtcatccccc tgctgctcaa
     3181 ggcgatgaaa ttcgatccgg cgctgggctc cgggatcgtc gtcaccggcc ttacggacgc
     3241 gtttggcttt ctttcatttc tcggcctcgc gaccttgttt attcattatc tgacttaaac
     3301 ctcagcgctg accgcccctg cggctacaga atctttcttg accagaaaaa gtatcccgcc
     3361 gattaaactg tccaccgcaa cgatgagaaa taacaataag ccaaaggcga taccctcctc
     3421 ggaatcgata ccgattaacg cgagcaaaaa aatataaccg ccttcgcgca agccaaaacc
     3481 gttaacgctg atcggcagcg ccgaaaaggt tcccaccaat ggataaacga taatgcaaaa
     3541 ggaaagcggc agactcaggt gcagggccag gcccatgatc acatgcatcc atgcttgaac
     3601 caggtgaacc atgaacgaaa gcgtaatcgc ctggggaatg acgcgccaac cttttcggta
     3661 actcgtgaga ataacccgca acttggtgat caacggatga gcatcctcag gcagcacgcg
     3721 ccgaaccagc ggcagcagca aaccgccgat caaaaacgcc acagacaaag agaacgtcag
     3781 cgcccgaatc gcacgaggaa cctcatattc aggaaacaag aggagaccaa gtgcgccaag
     3841 ccagatgagc accaccatgc cgatgccccg atccatcaac accgagacgg cggcttgaat
     3901 ggtgcttccg gatgcgcgct tgtgctggtc tctgttctcc tcccgggcaa gataataaac
     3961 acggctgacg tctcccccaa ccgtgctggg cgcgaacaga ttaaaaaaca tgccaataaa
     4021 atagtaagcg gtgagatcct tgtacggcgt cctaatgccc agggggcggg ccaggacagt
     4081 ccatctcagc gcgctgacca cttgggtgcc gagatatatc accagggcca atgcgatgta
     4141 ggaaaaattc gctgatgcca gagtatgggc aaattgctcg ccgtcgatgc gggttatgat
     4201 ataaccgaga agcccgacgc tgaccacgag cttgagaata aagatgacga tgcggttgcg
     4261 gttcttcatt cttcacaagc gggttggata agaacctgct tctcgtcctt cggcacatcg
     4321 tcgaccatca ctctacggcc agaaatgtga agtttccctt gggcataaag ctgaatcgcg
     4381 cgcggataaa tccgatgttc ctgtttgagg atgcgggcag cgagcgcggt ttcgctatca
     4441 tcctgaaaga ccggcaccac cgcttgcata atgatggggc cctcgtcgca ttcatcattg
     4501 acgaagtgaa ccgtgcaacc ggagaaccgc acgccatgtt ccaacgcttt tttctgaacg
     4561 cgcagcccgg gaaacgccgg cagcaatgcc ggatggatat tcataatacg attggaatag
     4621 gctttgatga acgggggcga caacagccgc atgaaaccgg ctagcacaac gagctcgacg
     4681 ccgcagcgcc gaagaatatc cactatcgcc tgatcgtatg cttcgcggct gaaaaacgat
     4741 ttatgatcca acactgctgt gtaaagactg tgcttctggg ctcgcgccag gccataagcg
     4801 tccgctttgt tgctcagcac gaccgcgatc ttagcatcga gcgcgcgcgc ttcgatagcg
     4861 tcaatgatgg cctgaagatt ggtgccgttg cctgaaatga gaacgcctat cgaaagctgc
     4921 cgtaccataa tctgtaggtt atatgatgtc cgcgccgcgc gggcctttgc ggatctcgcc
     4981 gatcaccatc ggcttttcgc cgctttgcct gagcgaacgc atggctccat ccgcggcttt
     5041 tttgccgaca actaaaatca tacccaaacc gttgttgaaa gtccggtcca tctcgtcacg
     5101 agatactgcg cctatcttgg ctatcatgcc aaatattggc ggctggggcc agctgtcccg
     5161 atggataagc gcgcgcactc cttggggcaa gacacgcggc aaattgccga cgattccgcc
     5221 accggttata tgcgccgcgc ctttgatgga cgacttagcc aacagattgc gcatggtctt
     5281 ggcgtaaatc cgcgtcggcg ctagaagctc ctcgcccaga gttcggccga gctcgggcag
     5341 tcgttgacca agtttcaggc cgccatgcga cagcaggatc ttgcgggcta atgaatagcc
     5401 attgctgtga agcccgctgg aacgaagtcc gatgagaaga tccccgggaa caatcgcttt
     5461 gggattgaga attttctttt tttccaacgc gccgaccacg aatcccgcca gatcgtactc
     5521 gccgtccggg aaatccccgg gatgctcagc ggtttcgcca ccgatcagta tgcagcctga
     5581 ttgcttgcaa ccttgagcga tgccacgaat gacagcctcg gcgataacga cgtcgagttt
     5641 gccgcaaacg aagtaatcca agaaaaacag tggctcggcc ccttgcgtca aaatatcatt
     5701 tacactcatc gccaccagat cgatgccgac ggtttcatga ataccagtgg cgaaagcaag
     5761 cttgagcttg gtgccaacac cgtcggtcga ggataccagg acaggttggt ggtagcgccg
     5821 ggctttcaaa tcgaaaagcg cgctgaaacc gccgacgccg gccagcaatc ccggcagttt
     5881 tgtttttttg gcaaccctcc cgatgcggcg caccagctca tcgcctgcct cgatgctgac
     5941 acccgccgcc gcgtaggtca ttgctcgttt cttggccaaa gaagtactgt ccgttctgcg
     6001 ttggtttgga gatattgaat atcgccgaat gaggccgcaa cgccgagagg gaaaatcatg
     6061 cggtggaacc ggcaatcgcc tctggcgttc ccatcgcgga ggcgagcaag cttctgattc
     6121 tcaatgtgta ctcaccgaag tgcacgctgc tcatcatctc cagagttcgc ggtcgggtca
     6181 gatcaatcga gagcacttcc accagcctgc ctggcctcgc agacataacg aagacgcggt
     6241 ccgacaaaaa caccgcttct tgaatgctgt gagtaacaaa cagaacagtt ttcttctttt
     6301 cttcccacaa tctcagtagc tcgagatcca attcgtcgcg ggtcatcgca tcgagagcgc
     6361 caaaaggctc atccatgagg agcaattgcg gatcggtcac taaagctcgg cacagtgaaa
     6421 cgcgctgctg catgccgccc gagagctgat gcgggtacat ttcctcgaac ccctgtagtc
     6481 cgactaaatt gatcaaagaa cgagccctct ccatgtggct ggcaacgttg cgcttggcaa
     6541 actccactgg caataaaatg tttcccaata ccgggcgcca ttttaacaac accggagcct
     6601 gaaataccat gccgacgttt tccaagggtt ggtcgacgga tctaccgctg atcagaattt
     6661 taccggatga cgcgggaagg agtcccgaga gaattttcag caaggtgctc ttgccgcagc
     6721 cgctgggccc tacgatggag acgaattcgc cggcgcgaat ttcgaaagaa atgcgctcta
     6781 aggcagtgac gatcttagcg ccgctggtaa acaccctgga ggcattgtca acggcgatta
     6841 gcggcggcat tcggacccaa actgagcgat gcaagtattt gtaagcgaat cgctacggcg
     6901 ttaagagtgg ctattcggag ttgaactacg gcgttttact atcttttgat aaactgattg
     6961 gtgaaaactt cctttgcggt aaccttgcgc gccacgtcga aatattcatt agtgatgcga
     7021 actgtcttat ccataatatc cggtttcatg taacccaagc cggactgcac cgattccgcc
     7081 ggcaacatga cctcgttaag agcatttctc aactgcagcg tcgtgagggc ttgatccaac
     7141 tcgggtttct gcttcagcag gatctttagc gcctcctctt ggttatcacg ggtgtagcgc
     7201 aatccttcca tggtgccttc aacataggcc ctgacgaaat caggccgttt tttgatatcc
     7261 tcttctttgg ccaccaaacc gctgccgtaa atgtccaggc cggtatcttt catgaaaacc
     7321 cgattcaact tggttccaac tttggctgct gccaattccg ccacctcgtc attggaagcg
     7381 cggaacatga tgataaaatc cgatttcttg gtcgcaagcg cggccggttg gatcggcgcc
     7441 gcggtctcct gaatcttgat agatttgaaa tcgatgccgt tgattcgaca ataagccggc
     7501 attagaaacc actgtgcctg cccggcgctg atggcccacg acttgccttg cagatcctta
     7561 attcccttta tcgcagaatc ttcgcgccac aagatggtag cagcgacata acccagctgg
     7621 cccaccacct gaacgccggt tccgcgggat cgcgcgacta tcgctgttga aatatcggca
     7681 aatccaaaat cagcccctcc ggccgctatc gtccgaacag tatccgccga acccgacccg
     7741 gcttggacgg tcgcggcaag accgcgcttg gcataaaaac ctttctcgag cgcgacgtac
     7801 catggcgcat gttttccgcc gatgataaag tccattctga tattggccgg tgtcggttgc
     7861 gcggctacgg cgttgaagct gaaagtgatc atcgccatga gcgcgcttaa aaccgctgcg
     7921 gcaacgcggt gaatgctaaa ctcaatgtca accctcgtca tacgctccac ctccgacgtc
     7981 agaaagaacc ttccacctga cgcacctcag cgctccacgg cgccaacttt ctttgaatga
     8041 tttcgaccag ccaaaaaagt cccagggaca gcaccgctaa aatcagcaaa ataaccaggg
     8101 caaatgctgt atccaaactg taattagctt gcaaaactag atagcccaaa ccttcatttg
     8161 cggcgacaaa ctcggcgaca atcgccccag tcaccgaaag cacgatcgcc actttgattc
     8221 cggcgaatag gtagggaagc gagttaggga gacgaatctt tttgaagaca tcgaattgag
     8281 aaccacgatt gatacgcgcc agctccaaca gttcaggctc gacttctttc aacccgacaa
     8341 cggtattcac cacaatcgga aaaaaggcaa cgaggaacgc aatggcaatt ttagacatca
     8401 acccgtatcc cacccatatc accatcaatg gcgcgaacgc gctctttggc attgcataga
     8461 gtattacgat gaggggataa atcgtattct gtagatgcgg cgaatagacc accgcgacgg
     8521 caaaagcaat ccccaacaat agagataagg cgaagccgcc aaaggcttcg ataaaagtga
     8581 tccaggcatg atggccgata accggcagct tttgtataaa ggagatccag agatcgcgcg
     8641 gcggcggcag aatgaaggaa ggaacctgaa taatctcgac cagaatttcc caaagaatca
     8701 gaagcgccag gaaaaatact atcggttcga tgatgttcct tgtactagcc ttcacgtccc
     8761 caactccgaa gccgacgtta aaggaaagct cctaccctgt caaggaaatg gcccgaacta
     8821 tggagctgaa aatctggttg caggctgctc aaattacccg gaggccgccc gaataactcg
     8881 tgttcgtggc cgtgaaaacg tgatccagac tccgaacgaa cacgaacacg gatttaaggg
     8941 gaaacaaacg gcatgtgagg gccttcgccg ggctgctaag gctcttccag ttcgcgcttg
     9001 agctggctgc gctcgagcag gacatcgcgt ttctggcgaa tcagctcgcg ctcttgtttt
     9061 aggaagcgct cgactgcacg acgaaatcga ccgtcgacaa tgtaatgcat gctggtggtg
     9121 tgctcggggt cgagaccgcg aagttgtttg aagctaccgc cggcgccggc ttcgaatcgc
     9181 tgcagtccca tggtgatgca gtgctcgatg gctgagtagt aacaaacatt gaagtggaga
     9241 aaaggctggt cttggaacgc gccccagtat cgcccataca tggccgtatc atctctgatg
     9301 ttaaaggttc cagcgataat cttgccgtcg ccttcggcca acatcaagca gatattgctc
     9361 gcgaacctta ggtgaagttg gtcaaaaaat tcctgggtga gatattggcg gccataatac
     9421 agtcgatcga tgtggccttt gtaaagacga aacatggtgc gcatatgttt atgtgtcagc
     9481 tcctcgccct cgatcgcacg gattgagatt ccccgctggg cgacttcccg ccgttcgcgc
     9541 ttgatcttgt tgcggcgatc gctgcggaag ctgccgagat aatcgtcaaa ggagtgaaac
     9601 ttatgatttt gccagtgaaa ctggatgccg cgccgggcga tgaatccaat ttgctcgaga
     9661 gccgccactt catcatccga gcagaagtta acgtgaactg aagaaatctt gttgtcgacg
     9721 gcaatcttgg ccagcgcttg ccccatgatc gtaattaaag cgcttcgatc ggttccggcg
     9781 cccgtcaaaa agcgattacc ggtcaccggc gtaaacggta ccccgacgag cattttggga
     9841 tagtactgaa ttccgacgtg atgggccgtc tcagcccact cataatcgaa gacgaactcc
     9901 cccatactgt gcagcttcag gtacatggga caggctgcaa cgacctttcc ggcgcgttcc
     9961 accaccagat gatggggcag ccagccagtg tcatcgttga cgcaaccgga ttgttccaag
    10021 ctgtccagcc agtgccattt taagaaaggc gagccgttgg ctatcaaagc gtcccattgc
    10081 acttgcgata tttccgagat cttgcgaaca gccctgatcg acacgtccac gctcatgaag
    10141 ccgactctat catgattgct gggaccagca agatttcgga gcttctggtg gaagcgctgt
    10201 atccattttg ttgatctcag ccgccttgac ttcatcccga aagatccttt aaatggttac
    10261 tctctagatg gcggacttaa gtttatggtg atttacaatc ttctctgcaa gaaaaaacac
    10321 ggttttgaag cctggtttcc tagtttcgag gagtatcaga aacaagccga gcaaaaactg
    10381 atttcctgtc ctacttgcgg tacgacaaaa gtcgaaaagg tgccgcatgc ctgcgctgta
    10441 catgtaaaaa aggagcagcc agctccgccg gccaagaccg cggcccaaac accgcccgca
    10501 ccgccgtctc cggctgaatt caaggagatg ctccttagag ttcatcacta cgtcaaacag
    10561 aatttcgagg atgtcgggcc acgatttgca caagaggcgc ggcagattca ccaggggaaa
    10621 gctgaggagc ggccgattta cggcacggct acggtcactg aggcagcaga gctcgccgat
    10681 gaagacatcc cccatgtagt tcttcccaaa cccgatttgg acagctagca ggccgctcaa
    10741 agagtctcat atgcttcgtt ccgctcgccc gactcacttc aacgtactga agcgtacgcc
    10801 tcagctcatc gaacatcgcg cgcctcggct ctgagtctct ttgagcagcc tataatcata
    10861 gttaaagcta cagcagtctc agtgactgtc gttagatccg gtttctttta ctccattcaa
    10921 gtaaaccctt cgcttacagt cggactcccc ttgcattgcc ggagcttctg cgaaatagag
    10981 aaagcttatt cctgcaactt cgacaggagg cgtcatgctg ctgatcaaca atcaaaccgt
    11041 cgagcagatt ctggacatga agggttgcat ggccgcgttg gaaaccggct atcgtgacct
    11101 catcgatgaa cgcgcagtct accgcggccg ttacgacctc ttcgtgccca acgatgaccc
    11161 gaagttgatg tatcgctggg gcaccatgga aggagcctcc agaagtttcg aaacgttcgc
    11221 cattcgcatg aaatccgaca tgctggaatg gcctgagggt aaaacggttg agaaatattg
    11281 catcgagccc ggaaccttct gcggcatcgt catggtattt tccactcgca acggcgaacc
    11341 tttggcaatc atcaatgacg gcgtcatcca gcatatgcgc gtcggtggct gcgccggctt
    11401 gggcgtgaag tatctatcgc gcgaagattc caaagtagtc ggcatcttcg gcgcaggcgg
    11461 aatggcgcga acttatctgc tcgctttcaa tgaggtcagg aaaattgccg aggtacgggt
    11521 ttacagcccg acaaaaaaga atcgtgacat ttttgcccaa gaaatgggtg aaaaattgtc
    11581 gatcaaaatc attccagtag aacgcccgga aaatgccgtg cgcggttgcg atatcgtcgc
    11641 cacctgcacc gattccattc aagttgtcgt cgatgaccct tcgtggatcg agcccggcat
    11701 gcacctcacc tgtgttaaat ccaacgaatg gaaccccgag atcgtcaaga actccgatct
    11761 cgtgatcaaa atgggccggg ccacgatcaa cctcgacgtc ggcatgcggc gcatcggcgg
    11821 cgaagccgcc gtggtcgccg gcaatgagga agagatcaaa agaatcgcca acccccgggt
    11881 cgatattttt tccggcgact ttcccgtgct cacggatgtc atgtccggca aactcaaagg
    11941 acgcaccgat cgcaatcagg tgaccttctt tgcgaacagc ggaactcagg ggttgcaatt
    12001 tgcttctact gccggctacg tcgttcgtga ggcaaaacgg cgcggcctcg gccaggagat
    12061 acccacccag tggtttcttc aggacatcag agactagctc tacatgcgga cgacgagacg
    12121 gccgactcct tagcgccact gcgaccgacg ttctcggctt tattggtgcc ataatcgtga
    12181 tccgacaaaa ttaaaaacca ataaactttc tctaatttga cagtgctctc cgtgatcagg
    12241 atgcgtagag tagccaccga gaaacgagat ggcggatgga agacgattcc caaatgatga
    12301 gagcctggaa cttagtttgg ggaccgcggc agcctatgcg atcctggcag gcatacaact
    12361 ggagttctgc acagcttgga cgagagtttc acaaccaccg agagctttcc gatagcgcgg
    12421 cgtcgatggt ggacggacgc ctgaggctag ttattccatg attgttagtt gtcgctggag
    12481 tgctggaagg aacgatcggg tttcccacgc aggccctgga gccgatcact tcgaatagac
    12541 agaacttgta atcggtgctt acattttgga aaatcgctgg cagaaatcgg ttttcagttt
    12601 ttcctcagat atggttttgc tgagagatgc aaccaactct ggaaaccgct cctgcggaaa
    12661 ttcctccatc gattctccga aggccctcac gtgatcacgc acaatcagag atgctatgct
    12721 gcgcatgaca ttgctgagct cactgaactc agcgatcatg cggtcgaaag catacggcga
    12781 cacggtttgc gaagcagctt ccgttatcgt ggacgtggct ccaatagcct gaaactcgaa
    12841 ggaggtcgat ctctctacgt cggtttccat cggctcgcgt ccggctgtgc cgctagtttg
    12901 cggcgcctga atatcaatca aagctgctgc tgtatcgtgc tttatcccgt tcgtgtgggg
    12961 ttctacgtgg acatctagat cgatgtcctg attccgatct tcgcatgatt gtttgatggt
    13021 cgattccgta tccgggaccc gaccgttcgc aacaagtcca gccacaattt gttccgtctg
    13081 gctaagttgg gcctgcagcg taacgatctt catcttgaga tcctcgataa cctgctcggc
    13141 ttgatgggcc ttactcgccg ccgtttcttt tgcgtgccca ttcgtcagct ccagatgcgt
    13201 tacctgatct accaaagcat ccatcttgga tttgagatcg tttacttcgg attgtcgacc
    13261 ctgcagagct tcttctttcg tcttcaatag actttctaaa tcgcggatct cggcggcgag
    13321 actgtcttcc atctttcggc tggcaatgtc ttttttatga atggtgtctt ccgtctctct
    13381 cagtctagcc tccagcgcgc tgatgtttgt cttgaggccg tcgataacct gctcggcttg
    13441 ctgattctta ctcgcagcct ctcctcttgc gtgctcgata gccaactcca aatggctgac
    13501 ctgcccggcc atgacatcta ttttcgagtt gagtacgttt atctcggatt cccggcgttc
    13561 cagagcttct tccttcgttc tcaccacatt ttgtaaatcg cggatttcgg cattaaggtt
    13621 ttcttccatc ttttggctgg cagcttcttt tctatggacg atgtcctcgg tctctcgaag
    13681 cttagcctcc agccctgcga tgtttgcttt cagaccctca atctcgtgct cggcacgttg
    13741 cgcctcacgc gccaccacgg tttgatcgtc gctaaccgcc gccttcaaac taccgattcg
    13801 gtcgaccaag attttctcga gctccccgat cgcatcgttt aaactgccgt cgctatcttt
    13861 tacccgcgcg gaaatcatga ggtgcggttt ccgccggtgt agagtccctt gcactacgcc
    13921 tctgactgat tgcattactt tggtcatatt tcctccgagc gtaagtgtcc aaacttttgt
    13981 cactgcttgt caataaaatt ctctacgtta caactgggca gccacatttc tgggatcaac
    14041 tggactgtgt ctcatggaac accatggcat ttcttgctgc cctttgttca tttaagccgt
    14101 cctgatgcca tatcatcgat gccggtgaca cgagacagta ctacaaacag ttcacatacg
    14161 cgttcaactt tcgttggatt cgtgtcgctt cttccaggat tacggctatc ggcgtcgcgc
    14221 tttggtgtgg acggcacgaa tgcaacgtcg agcaggaaga taacaccggt taacgaaacc
    14281 gagcttgacg atgaaacctt ttcatgtcga gcaaacacag caggggcttc aatcgcggca
    14341 gtcagctggc cgccggtcca cctggccggt actcctgtca ccgtttagga gctcggctaa
    14401 gtgtcggttt gtggggattc cgtcaggatt tcgtgtaggc ggccgtgatc gataggcttg
    14461 gccaggtgat aatcgaagcc agcctctcga gagcggaggc ggtcattttc cattgcccag
    14521 ccagtctgcg cgatcaatac cgttttgctg aattgcggct gctctcggat tcggccggca
    14581 aggccgtagc cgtccatacc aggtaaaccg atgtctatca gcgctacctc cgcggggaac
    14641 tcgtgcagta tttccagcgc atctgcgccg ttatgtgcga tacgcacatc atgccccctc
    14701 agttgaagga gaatggctaa actcttcgcc tggattgcga ggtcatcgac gaccagaatc
    14761 cgacggcctt tttcgccgac agtttgtgca ttcggtcgta cggcaccatc ctgatcttcg
    14821 accacgggat cgccttcgcc aattggtatt cggacaacaa attcgcttcc acgacctaag
    14881 ccctcgcttc ttgcctctac attaccgccg tgcagctcaa caagactgcg caccaatgtc
    14941 agtccgagac caagaccgcc tcgttcg
//
LOCUS       DASUVS010000025         8124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_444058, whole genome shotgun sequence.
ACCESSION   DASUVS010000025 DASUVS010000000
VERSION     DASUVS010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8124
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444058"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..585
                     /locus_tag="VF182_01320"
     CDS             247..585
                     /locus_tag="VF182_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435739.1"
                     /translation="MKISELGFCGFNHITENNNLLMALKQTRDKSPERRKPRACPECG
                     STEVIPIVHGIPTPALQKLIDEDKAIIADREEWEGMSEWYCKACGCDWSGEWRRFKMP
                     GGVNATRVKK"
     gene            531..1589
                     /locus_tag="VF182_01325"
     CDS             531..1589
                     /locus_tag="VF182_01325"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435740.1"
                     /translation="MAQIQNARRRQRDPGKEITRRVNRHDAVIFTLLILVSAANSLRP
                     PTAFSQSTSDKRASAAIIYEAIAATNAPLWLMEDARLFEKYRLNGKVIHARGAVPVQA
                     LVSNSVEFGAFGGPSAVAAVLAGSDLVLIAAKGNFAVMSIWVRQDSPIRTIADLKGKT
                     VGVTRAGSATHTIARLALRSGGIGDNEVKYLHHPGLPEAFVSLDRALVDASFGSPPRP
                     GFRELVDLSSLKIPFLQGAIEVQRSFLHNQRDTALNLLRAYVESIKLAKERPELIIAS
                     IAKRMRVPVELVRPAYQPHANVFEAIPYVRKDSLQAILDLYPKDQLRNAALEKLVDNS
                     LLQQLEDNGFIKELYSKN"
     gene            complement(1738..2394)
                     /locus_tag="VF182_01330"
     CDS             complement(1738..2394)
                     /locus_tag="VF182_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435741.1"
                     /translation="MKTYKTHYLLFLIVLSACSSSKTVSPGMTLLTGLNGYHDEMEEL
                     EGQEARWPERQRIGNTIRTTYLTTMGGSKEFNRLVELDLKRREYRIALRANSLRPDRA
                     AEVKQELVGIDAEIAALGTLVKKQVAARSMPEPEPQPQQVIEGVATIGLLTMAIDEFS
                     PSLPRYAAVPSVQVGGYTVIDQRKFTIVKTPEGQTFHCTTVLVQEEGAGINCGALAGK
                     "
     gene            2784..3878
                     /locus_tag="VF182_01335"
     CDS             2784..3878
                     /locus_tag="VF182_01335"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6435742.1"
                     /translation="MSIPTEQKIIDGDGHVVEDIATIWKYMPEQYVGRSFSDARGKSP
                     FPPIDHMHTANRHFTPRGAFANVGREGWELFLDDVGIGTTVLYTSAGLAFGKIVSRDW
                     AIELARAYNNWIHDTYVSKSPRFKAMGLVPLQEPAEAMIELRRIVRDLGFCGAMLPSM
                     GANMPHLGSEKYWPIYGEAERLGCAIAIHGGAHEGLLMDDLSPYAPVNALGHPMGQMI
                     AFAGIIFNGIFDKFPGLRIGFMEAGSAWLLTCLERFTGSWESHVQFDPRGRFLQLRKG
                     EKIIDYVCRHIDEGRIFLGVEGEELTIAEALRIVGNKPFVYSTDYPHEVDADTCKHEL
                     EELRENPEITAADKEAILFRNAQRFYQLTQ"
     gene            3946..5064
                     /locus_tag="VF182_01340"
     CDS             3946..5064
                     /locus_tag="VF182_01340"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6435743.1"
                     /translation="MSNTKHKIIDGDGHVVEDHSAISARMPEDYRGRFGSGRSPYPPN
                     DHLHAANAHFLPPGAFAKVGREGWLEFMEDVGLDSAVLYPTNGLSFGRVISKDWAIEL
                     ARAYNDWMHDEYISKSSRFQALGLIPLQEPPEAVKELRRIVKELGFCGAMLPSTGPMQ
                     SQNHLGDERYWPIYEEADRLGCAIGIHGGVHDHMGLDDMSPYAPVNALGHPFGQMVNF
                     AGILFNGVFDKYPNARFGFMEAGAGWFVNCVERFERSWNSHVQYDPRARFLKLRDKES
                     ITDYIKRHVDEDRIFIGVEGDELTLPFAVSIVGNKPFIFSSDFPHEVNNDTCKAELEE
                     LNENKRLTEDDKDAVRYRNAERFYGLRPNEGNSRPRSN"
     gene            5099..6103
                     /locus_tag="VF182_01345"
     CDS             5099..6103
                     /locus_tag="VF182_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435744.1"
                     /translation="MKSKCAGAGIHFILVLTTLFLELVASAGSHAQSLTTFRIANGTS
                     GETPAVLWVGVDQGFYRKHGLNVETIFMRSGPLAMSALASADVQMVFTSSNNVLNVAA
                     GGMDAAVIGTVVGRPEGDFMARPEIKKPEELRGKQLAIQSIGGGGWANNMLALDYLGL
                     DPERDNIRFIVLGDQPSRIQALETGRAQASWMGSTFSAPLKKKGYTVLLDLARAPISY
                     LGTSLVARKSAVRQEPKLYESMLKGTIDAMRFFMKPENKPAVMQSMARVLRLPRVDDA
                     ENGYNALLSVYTNDMRPKVEGVKKIHSILARTNPKLQKLKSEEIIDDSWIRKIVESGY
                     "
     gene            complement(6253..6444)
                     /locus_tag="VF182_01350"
     CDS             complement(6253..6444)
                     /locus_tag="VF182_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020405533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6435745.1"
                     /translation="MPEQLMTLEQVAKYLNVDKFTVYRLLADRELPAFKVGNQWRFKK
                     KLIENWLRKNSNLQKKRSQ"
     gene            complement(6687..7034)
                     /locus_tag="VF182_01355"
     CDS             complement(6687..7034)
                     /locus_tag="VF182_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6435746.1"
                     /translation="MDIQRINPREWNCSATVLGDLVFLSGTVADNKSLPMKGQTEEVL
                     RKIDAVLAKAGTNKSRILSATVYMADAALKDEMNEAWMAWVDRSNLPTRTAVGAALTP
                     GTLVEITVCAAKS"
     gene            complement(7086..>8124)
                     /locus_tag="VF182_01360"
     CDS             complement(7086..>8124)
                     /locus_tag="VF182_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX6435747.1"
                     /translation="AIIMGKLHMSSLPDGSLPVPRNPWNTEHITGGSSTGSGAAVSGG
                     LCLGSLGEDTAGSIRNPSAFCGISGLKATYGRVSRYGLAPLSWSLDHCGPMARVVEDT
                     AHILEAVAGHDAKDPTSSSVPVAEYAGALREDVRGMTVGVPRDFIDECAARTDPIVIK
                     LVNRAIEDLKKLGARLEEVKVPTLNLATIANAVIYYNEFWAAHKSDAASVLKNGAAQR
                     RARIYLGLLTGSADYIQAQRVRSRVRREFAEVFEKVDCLALPCQAGPAPLVKEVGPID
                     TLFKHVVPEYHGPFNLTGLPTLSVPCGFSDSHLPIALQLVGKPFDEVTILRAGYTYQQ
                     SAKWYEQRPPL"
BASE COUNT         1869 a         2300 c         2172 g         1783 t
ORIGIN      
        1 ccatcagccg ggaactcgcc gatgtcaaag caaagctact cgtcgatgtt gccaaagagg
       61 tgcgcaagtc gatttagtat cacctagaag ttcgttgcag attcttagat gctttcgtcg
      121 tcattcccgt gaaaacggga atccagatcc tccctgtctc tccccttctg taagggggag
      181 actaaagagg gggtcgattc cgggctgctt actccggacc gtgatccgta atagcgccgc
      241 aagacgttga agatttcgga acttggcttc tgcggcttca atcacatcac tgaaaacaat
      301 aatttactca tggcgctaaa acaaacccgc gataaaagtc ccgaacgtag aaaaccccgc
      361 gcgtgtcccg aatgcggctc caccgaagtg attcctatcg ttcacggcat tcccacgccg
      421 gcactgcaga aactcatcga cgaagacaag gcgatcatcg ccgatcgcga agaatgggaa
      481 ggcatgagcg agtggtactg caaagcttgc ggctgcgatt ggagcggcga atggcgcaga
      541 ttcaaaatgc ccggcggcgt caacgcgacc cgggtaaaga aataactcgc cgcgtgaata
      601 gacacgacgc ggtcattttt actctgctga ttcttgtgtc cgcagcaaat tctctgcggc
      661 ctcccacggc atttagccaa agcacgtccg ataagagagc ttcggccgca atcatctacg
      721 aagctatcgc ggcaacgaac gcgccattat ggctcatgga agacgcacgg cttttcgaaa
      781 aataccgcct gaacggcaag gtcatccatg cccgtggcgc ggtaccggtc caggcgctcg
      841 tcagcaactc ggttgaattc ggcgcgttcg gcggaccttc cgctgtggct gcggttctgg
      901 ccggcagcga tcttgtattg atcgccgcca agggcaattt cgcggttatg tccatttggg
      961 tgcgtcaaga ctcgccgatt agaaccatcg ccgatttgaa gggcaaaact gtgggcgtta
     1021 cccgcgcagg ttccgccacc cacaccatcg ctcgcttagc cttgagatcc ggcgggatcg
     1081 gtgacaacga agtcaaatac ctgcaccatc ccggcttacc ggaagctttt gtttcactcg
     1141 atcgagctct ggtggacgcg agcttcggct caccgccgcg tccgggattt cgcgagctgg
     1201 tcgacctgtc ctcgctaaaa ataccgttcc tgcaaggcgc catcgaggta cagcgcagtt
     1261 ttctccacaa ccagcgcgat acggcgctta atcttctacg ggcatacgtc gagagcatca
     1321 agcttgcgaa agagagaccc gagctcatca tcgcttccat cgcaaaacgc atgagagtcc
     1381 ccgttgagct cgtgcggccg gcatatcaac cgcacgcaaa tgttttcgaa gcgattccct
     1441 atgtccgaaa agattcgctg caagccattc tcgatcttta tcccaaagac cagcttcgca
     1501 acgcagcgtt ggaaaagctc gtcgacaaca gcttgttgca gcagctcgaa gacaacggct
     1561 ttatcaaaga actttattct aagaattgat tcttgttcga gcggacgagc gattaagcac
     1621 tgacgtgttg acgtcagggt gctcagagac attcacatgt ccattgctca acgccgatcc
     1681 tggaatgctg atacacaaac acaagcagct tcaagtttat aaacagatgc aggcggtcta
     1741 tttaccggca agcgcgccgc agttaatccc cgctccctct tcttgcacca aaaccgttgt
     1801 gcagtgaaaa gtctggcctt ccggcgtttt tacaatcgta aactttcttt gatcgatgac
     1861 ggtatagccg cccacctgta cgcttgggac ggcagcgtac cgcgggaggc tcggagagaa
     1921 ctcgtcgata gccatcgtca gcaaaccgat cgtggcgaca ccttcaatca cctgctgcgg
     1981 ctgcggctcc ggctcgggca tactccgcgc ggcaacctgt tttttgacaa gcgtcccgag
     2041 cgcggcgatc tccgcatcga tcccgactaa ctcctgcttg acctccgcgg cgcgatccgg
     2101 tcgcaacgaa ttggccctta aagcgatccg atactctctt cttttgaggt cgagttccac
     2161 cagccgatta aactccttgg aaccgcccat ggtcgtcaga taagttgtcc tgatggtatt
     2221 gccaattctc tgtcgctccg gccatcgagc ctcctgcccc tcgagctctt ccatctcgtc
     2281 gtgataacca ttcaagccag tgagtagggt catgccggga gagacggttt tagaggacga
     2341 acacgcactg agcacgatca aaaacaataa gtagtgggtt ttgtaagttt tcatccgagt
     2401 ccttcccctg tcgttcgagc gacgggtggc attttaacac gagataaaat cggttgtgag
     2461 attttctcaa atcgcgataa aaacgcggtc gtgcaaggcg tctaacgtct ggaccggtgc
     2521 ctgctttcgt tataaagaaa gcatgaagcg cagcgcggta gtttgcggcg gtcagaggtg
     2581 actacgagaa ttaaccaaga atcgtcaccc ccgaatgtct taatcggggg tccagttccg
     2641 atctcacctg gattcccgat aagaacattc gggaatgacg catctgagag gtccaggaat
     2701 aatatcttac ggcgcaaggt gcgcgggatt aacgcagcga tcggaacgag caacaatttc
     2761 gcgaagtagt gaaggaagaa ccaatgagca tacccactga acagaaaatc attgatggcg
     2821 acggccacgt tgtcgaagac atcgccacca tctggaaata catgcccgag cagtacgttg
     2881 gcaggagttt ttcagatgcc cgcggcaaga gtccttttcc acccatcgac catatgcaca
     2941 ccgccaatcg ccatttcacc ccgcgcggcg cgtttgccaa cgtcggccgc gaaggttggg
     3001 agttgtttct cgatgatgtc ggcatcggca cgaccgtgct ctacaccagc gcgggactgg
     3061 ctttcggcaa aatcgtcagc cgtgattggg ccatcgagct agcccgggcc tacaacaatt
     3121 ggatccacga tacctacgtc tccaagagcc cgcgtttcaa agccatggga ctcgttccat
     3181 tacaagaacc cgccgaagcg atgatcgaat tgcgacgcat tgttcgcgat ctgggttttt
     3241 gcggcgcgat gttgccgtcg atgggcgcga atatgccgca cctgggatcc gagaaatact
     3301 ggcctatcta tggtgaggcc gaacgccttg gctgcgccat cgccatacac ggcggcgccc
     3361 atgagggctt gctcatggac gatctaagcc cctatgcgcc cgtcaacgca ctgggccacc
     3421 ccatgggaca gatgatcgca ttcgccggta ttatctttaa cggcatcttc gacaaatttc
     3481 ccggattgcg aatcggcttc atggaggcag gttcggcatg gctgctgacc tgtctcgagc
     3541 gcttcaccgg ctcctgggaa agccatgttc aattcgatcc tcgcggccgt ttcctccaac
     3601 ttagaaaagg cgaaaaaatt atcgactacg tctgccggca catcgatgaa ggacgcattt
     3661 ttctcggcgt cgaaggcgag gaattgacca tcgcggaagc gctacgcatt gtcggcaaca
     3721 agccgtttgt ttattcaacc gactatcctc acgaagtaga cgccgatacc tgcaaacacg
     3781 aactggaaga attgcgcgag aaccctgaga tcacggccgc cgacaaagaa gccatcctgt
     3841 ttcggaatgc gcaacgcttt tatcagttaa cgcagtgaat cgtacggccg ggtttaaacc
     3901 cgccgatggc cgcacgatca tgacattcag gctgaggatc cgaacatgag caacacaaaa
     3961 cataaaataa tcgatggcga cggacacgtt gtcgaagatc actccgccat ctcggcgcgc
     4021 atgcccgaag attatcgcgg ccgtttcggc tcgggtcgtt caccgtatcc acccaacgat
     4081 catcttcatg ccgccaacgc ccattttctc ccgcccggcg ccttcgccaa ggttggccgc
     4141 gaaggctggc tcgaattcat ggaggatgtc ggcctcgaca gcgccgtgtt gtacccgacc
     4201 aatggattgt cctttggacg ggtgatcagc aaagactggg cgatagaatt ggcgcgcgcc
     4261 tataacgact ggatgcatga tgaatacatc agcaagagtt cccgcttcca agccttggga
     4321 ttgattccgc tccaggaacc gccggaagcc gtgaaggaac ttcgccgcat cgtcaaagag
     4381 ctcggcttct gcggcgccat gctgccgagc accggcccga tgcaaagcca gaatcaccta
     4441 ggcgatgagc gctactggcc gatctacgaa gaggccgatc gcttgggctg cgccattggc
     4501 atccacggcg gcgttcacga tcacatgggc ctcgatgaca tgagtcctta cgctccggtc
     4561 aacgcgctgg gccatccatt cggtcagatg gtgaacttcg ccggtattct cttcaacggt
     4621 gtcttcgaca agtatcccaa cgcgcgcttc ggcttcatgg aagccggcgc cggctggttc
     4681 gtcaactgcg tggaacggtt tgaacgatcc tggaacagcc acgtacagta cgatccgcgc
     4741 gctcgctttt taaaacttcg cgacaaggaa tcgattaccg attacatcaa acgccatgtc
     4801 gatgaggacc ggatcttcat cggtgttgaa ggggatgaat tgacgctgcc cttcgccgtc
     4861 agcatcgtcg gcaacaagcc atttattttt tcgtcggatt ttccccacga ggtcaacaac
     4921 gatacctgca aggcggagct cgaagaattg aatgagaata agcggcttac tgaagacgac
     4981 aaggacgcag ttcgctaccg caacgcggaa cgtttctatg gcttgcggcc caatgaaggc
     5041 aactcaagac ctcgatcgaa ctgagccaaa agaatcccga tccattgtgg agtcaacctt
     5101 gaaaagtaaa tgcgcagggg caggcatcca ttttatcctc gtattgacca ccttgtttct
     5161 cgaactcgtt gccagcgccg gatcgcacgc gcagagcctc accacgtttc gcatcgccaa
     5221 cggcacttca ggcgaaactc cggcggttct ttgggtcggc gtcgatcaag ggttttaccg
     5281 caaacacggc ctcaatgtgg aaacgatctt catgcgcagc gggccgctgg cgatgtcggc
     5341 gcttgcctca gccgacgttc aaatggtttt taccagttcc aacaatgtcc tgaatgtggc
     5401 ggccggaggc atggatgctg cggtgatagg cacggttgtc ggccggcccg aaggcgactt
     5461 tatggcgcga ccagagatca aaaagcccga agagcttaga ggcaagcaac tcgccatcca
     5521 aagcatcggc ggcggtggtt gggccaacaa tatgctcgcg ctcgattatc tcggtttaga
     5581 tcccgaacgc gacaacatcc gattcatcgt tctcggcgat caaccatcgc gaattcaggc
     5641 gctcgaaacc gggcgcgccc aagcttcatg gatgggctcg acgtttagcg cgccgctcaa
     5701 aaagaagggg tacacggttc tgctcgattt ggcgcgggcg cccatatcgt acctgggaac
     5761 gtctttggtc gcgcgcaaga gcgcggttcg acaggagccc aagctatacg aatcgatgct
     5821 caaaggcacg atcgatgcga tgcgattttt tatgaagccg gaaaataagc ccgcggtcat
     5881 gcagagcatg gcgagggtgc tgcgtcttcc acgggtcgac gacgctgaga acggctacaa
     5941 tgcgctgttg tcagtctaca ccaacgacat gagacccaag gtcgaaggcg taaagaaaat
     6001 ccattcgata ctggcgcgca ccaatccgaa gctgcagaaa cttaaatcgg aagagatcat
     6061 cgacgattca tggattcgca aaatagttga aagtggctat tagctgctcg ggtatctact
     6121 aggacgtcgc agaaattacc gctcgaacat gaactgagaa gagcttcgat tgcagctgca
     6181 atccagggtc agtctaaaca tcggcgactg atctgccgct tcggcgagga aagaacgaat
     6241 cggggaatgt cgctactgag aacgcttttt ctggagattg gaatttttcc tgagccagtt
     6301 ctcgatcaac ttctttttaa agcgccactg gttaccgact ttgaaagccg gcaactctct
     6361 atcggctaac aagcgataaa ctgtgaactt atcgacattg agatatttgg cgacctgctc
     6421 tagcgtcatc agttgttctg gcatgttcgt agccctcttt ggaccttatc tgatcccgat
     6481 ggcgtagctc cacgtaaacg cttagagacc catatttagg gagcgtcaaa tattctaaat
     6541 ggcagataag gtcaaggccc cggggggacg aacgtcgcgt agtcaccctc gtctgttaga
     6601 tgagctatcc ggtgtaagaa ccatttacac ttctctccgg tacggtgaac ggcacggcat
     6661 cgggtcgatg ccgtgcctct gacggactag gatttcgccg cgcacacggt gatctcgacc
     6721 agagtgcccg gcgtgagggc cgcgcccacc gccgtcctcg tcggcaggtt cgacctatcg
     6781 acccaggcca tccaggcttc gttcatttcg tccttaagcg cggcatccgc catatagacc
     6841 gtagcgctga gaattcgaga tttgttggtg cccgccttgg ccagcaccgc atcgatcttg
     6901 cgcaacacct cttctgtctg tcctttcatc ggcaacgact tgttgtcggc tacggttccc
     6961 gaaaggaata ccaaatctcc taagactgta gcgctacagt tccattcgcg tggattaatc
     7021 cgttgtatgt ccataatatg ctttgctcct ctttagtttt tgaactgctt gaacggttga
     7081 acttttcaaa gtggcggacg ctgctcgtac cacttcgcac tttgctggta ggtgtacccc
     7141 gctcgcaaga tcgtcacttc atcgaacggt ttgccgacga gttgtagagc gatcggcaaa
     7201 tgactgtctg agaatccgca aggaaccgac aatgtcggca gtccggtcaa gttaaatggc
     7261 ccgtggtatt cgggaaccac atgtttgaac aatgtgtcga tgggtccgac ctctttgacc
     7321 agcggcgccg gacccgcctg gcatggcaac gccaggcaat ccactttttc gaagacctcg
     7381 gcaaattccc ttcggacgcg actcctaacg cgctgcgcct gaatgtaatc cgctgaaccg
     7441 gttagaagtc ctagatagat tcgcgcgcgg cgttgcgcag cgccgttctt cagcaccgag
     7501 gcggcgtcgc tcttatgcgc ggcccagaat tcgttgtaat aaataacggc gttggcgatc
     7561 gtcgcaagat tcaaggtcgg aactttgact tcctcgaggc gcgcgcccaa ttttttcaaa
     7621 tcttcaatgg ctcggttgac cagtttgatg acgatgggat cggtgcgcgc tgcgcattca
     7681 tcgataaagt ctcgcggcac gccgacggtc attcctctga cgtcttcgcg caatgcaccg
     7741 gcatattcgg ccaccgggac actgctcgaa gtcggatctt tggcatcatg accggcaacc
     7801 gcctccagta tatgggcggt atcttcgacc acacgcgcca tgggaccgca atgatccaat
     7861 gaccaactca gcggcgctaa gccgtaacgg ctcacccggc cgtaggtcgc ctttaagccg
     7921 gagatgccgc agaaagccga ggggttgcga atcgagccgg cggtatcttc gccgagagag
     7981 cccagacaga gtccgccgga gacggcagcg ccggaacccg tgctcgaccc gccggtgatg
     8041 tgttcggtgt tccacggatt gcgcggcacc ggcagcgacc cgtcaggcaa actgctcatg
     8101 tgaagcttgc ccatgatgat cgcg
//
LOCUS       DASUVS010000026        54055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_7235, whole genome shotgun sequence.
ACCESSION   DASUVS010000026 DASUVS010000000
VERSION     DASUVS010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 54055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54055
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7235"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VF182_01365"
     CDS             <1..474
                     /locus_tag="VF182_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435748.1"
                     /translation="VFPTKADIHTPEITSSHLKGMAHYFGADLVGVVKLPAKKRSPSP
                     LPQAADGKIEEEYPFAVVCIVRAEYHPRSAPGIGGQVPVQNGLFITFVLSAWIRELGF
                     RATVAQETNGEDLAAAAGLGKINGNGRLVTPEYGADVHVADIILTDLPLAPDGEK"
     gene            672..1334
                     /locus_tag="VF182_01370"
     CDS             672..1334
                     /locus_tag="VF182_01370"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX6435749.1"
                     /translation="MPYVITSLCTNDGACVDVCPVACIHTTPGAPQFYIDPEVCIDCE
                     QCEIVCPVDAIFKDIDIPAEHQAAIEVNAAFFRKNKAVAGPVSFDTAWEMIRAAHDYA
                     QIMGLSVTTAVVDEAGGPIALGRMDNADPVTAELAFNKAYTAAAFHVATAELAPQGRQ
                     ARFRSLTISHRGRIMPVGGAIPIIDGVTIVGAVGVAGASRPEQDILCCRAALAVIEGH
                     GH"
     gene            1353..1661
                     /locus_tag="VF182_01375"
     CDS             1353..1661
                     /locus_tag="VF182_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435750.1"
                     /translation="MYLVQLLLPLYDNLNARFPPEAFDRVRQELTEHFGGVTAFLRSP
                     AQGFWRIAENAINRDEVVIFEVMTDELNRQWWDGYRADLEQRFRQIELVIRASSIERL
                     "
     gene            1963..3462
                     /locus_tag="VF182_01380"
     CDS             1963..3462
                     /locus_tag="VF182_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435751.1"
                     /translation="MVRHRRPFTIVYMKEIRFVDTTLRDGQQSLWAYGMRTGMIVPIA
                     EHIDDAGFEAIELGGPVELPKCVRELREDPWERYRLVFARIKKTPLRVIHGTRSGFAI
                     YPHAAHQLYDRCMGDIGIKQVRISDSWNDPADWQWRVKQARAAGLEPIINLIYTISPR
                     HSDEYYAQKTREALKLNVFRVCLKDPGGILTPERTRTLARAVLQSAKDTMAELHTHCT
                     TGLGSLCCLEAIEAGMTSINTAIPPLADGSSNPSIFNVARNAKAMGYAPIIDEEPIHA
                     VAAHFTAIAKREKLTIGKPPEYDYAQYIHQIPGGMISNLRHQLRLVGMEDKLERTLDE
                     AAQVRAEFGYPIMVTPLSQFVGSQAAINVIVGERYKEVTDQTIEYALGIWGKEGGALM
                     DPDIKDKILSRRRADEIGKRAVPQMSLQEFRRKFGGSGVSDEEVLMRFFTSKEDVDTM
                     RAAGPARVYPNGGNPLLHLLEELTRTTNRSSISVRHGDVSVHLKRNTAS"
     gene            3687..4334
                     /locus_tag="VF182_01385"
     CDS             3687..4334
                     /locus_tag="VF182_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435752.1"
                     /translation="MEKFKVSYSLKLGLALGLSLLLGCVAGPTLHETRRGESLPGPDA
                     TLQPGEIRAEVVQVTPNRNEIQVRADDNRSRVLRYDPGRTRVTYQGRDYSVENLEAGD
                     VIAYQSAGRDSTTIDTIRVQQPVQARAASTPGRPGSSLTRGEVLEGTVDRVDYDRGIF
                     DVRTRDRGTITVALPFNARPSDVDAFRRLRNGDSVRLEGEFVNRDNFQVSSISNR"
     gene            complement(4364..4792)
                     /locus_tag="VF182_01390"
     CDS             complement(4364..4792)
                     /locus_tag="VF182_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6435753.1"
                     /translation="MAYDIKKVDVWAGEIADRPGGLGATVEALSNAGANLEFLISRRA
                     PDRPGTGVVFLTPVKGTKQRNAAQSAGLSTTDSLHSVRVEGPDKPGLGTQLTRALSDA
                     GINLRGVSAAALGRRMVTYLAFDNARDADNAIRALRKALK"
     gene            complement(4946..6310)
                     /locus_tag="VF182_01395"
     CDS             complement(4946..6310)
                     /locus_tag="VF182_01395"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6435754.1"
                     /translation="MEATAQIARFVVDTEFQKIPAQAVKLAKTAMLDCLGVALAGSKE
                     SSARICAEIVRQEHAKEESSVIGQGFKSTAIHAAFANGTAAHALDFDHSFTLMGQPTA
                     PIIPAIISLGESLGASGRRCVEAYVAGFETITKLVMSLRQRSEDGWHPPSSFGSFGAG
                     VACAKLQGLNQSQVEMTLGTAASMASGLVCNFGTMSKPLHVGLGARNGVVASKLAQSG
                     FTANNQAIETGLGFYEVFYPGAEPDKRPLEELGKIYELINSGIRIKPYPCGGLTHPAI
                     DGVLEFKTKHGITAEMIESIDVGVARHTFERIVFRVPENGLQGKFSMPYLLARAIIDG
                     RLFLDAFTDSAVKDKNVLRLAEKIQMHLEADLKPTALGSRPCKVTIRLRDGRSFSRQI
                     DYAKGSREAPMTDDELKQKFADCAREALDDKSVERITDYVEHLEALDDIRPLCQLLIG
                     PNRS"
     gene            complement(6355..7413)
                     /locus_tag="VF182_01400"
     CDS             complement(6355..7413)
                     /locus_tag="VF182_01400"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6435755.1"
                     /translation="MTRHRSRVIAGLILTVALAVLPLFDSAVFAQADFYKGKTIRIMV
                     GSTPGSFYDLWGRLLARYWGKQIPGNPEVIVQNMPGAGSLTATNYVYAVAKPDGLSMV
                     LPNNSIYIEQLVERKEVQFDLRKFHWIGSASQDSIIFYMRADTPFKTVGDIVKAKEPP
                     SCGGSGTTSSDYLVAKILELTLGANINSVLGYPGGNEADLAVEKGEIACRAHTLAAHF
                     GREPFNSWHKKGFDRHLLQTGRKRDTRAAEAPTIYEILEEFKVPASNRRIVQAMLSGG
                     EFGRPFLLTPGTPPERVKILRESFVKVLKDPELLEDAKKAKMEVDYTSGEELEALIKE
                     VLDQPPEIVQQVRKFMAK"
     gene            complement(7607..8332)
                     /locus_tag="VF182_01405"
     CDS             complement(7607..8332)
                     /locus_tag="VF182_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6435756.1"
                     /translation="MADYILKRKDADVTTLTLNRPDIGNRQDDATLAQLAQMIDDAAK
                     ESRLIVFKAAGEDFCLGREAMGKPGPALEAYQMRERAETIFDLYESFRRSKVPIIGVV
                     QGRAAGLGCALAALCDITVASDKARFQLPEMAHHIMPTIAMSALVDRVPRKAATYLIY
                     STQEIDANKALMFGIISDVVPAAELDSAVGKLLERLKQTPLPALMAVKEYGRSAFNMN
                     TQAATDFARNLHATVNTFSGMKP"
     gene            complement(8416..9630)
                     /locus_tag="VF182_01410"
     CDS             complement(8416..9630)
                     /locus_tag="VF182_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX6435757.1"
                     /translation="MTLLPRFYHVALARPFWTGATFRAILRCLLSGRVIAGRELTELK
                     GCISEELGVQNAILCGSGSLALELALRSCDVKSADEVVVPTFCCSSVIAPILAVGACP
                     VLADVGDELNLTARTVEPALTKRTRAIIVPHLFGNPAEIKSIIDLTRGTDIRVIDDAA
                     QALGASLDGRILGTFGNAGILSFGTAKVCSGLGGGVLISRHREVFDQDAEIELAAADL
                     SLALGSLSSTLIWHRWRRWSVPLHEALHGSSPTDPESLPPPYQKESMSNLKAAVASSL
                     MKSLQANLAARRARVRAYHGLLGNEDLLLVSHREGSACLTQVIRVLPKSTGHDPASDL
                     IASLRGAGFEVQGSYIPIHFLAPYQQLAVRSFPHAEKIWTDLVELPCEPGVSFDQVEQ
                     IASIVKQTFRCS"
     gene            complement(9891..10265)
                     /locus_tag="VF182_01415"
     CDS             complement(9891..10265)
                     /locus_tag="VF182_01415"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6435758.1"
                     /translation="MRVLKISELTEKPMDTATPVPGWSGGNVSRTRQAIIGETDSEDF
                     RCNVVNFRQGATTGWHVHDCDQILVITAGRGSVANDKEQREITVGDVVHIKAGERHWH
                     GANADTPMSHITVTKAGSQSKH"
     gene            10615..11589
                     /locus_tag="VF182_01420"
     CDS             10615..11589
                     /locus_tag="VF182_01420"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX6435759.1"
                     /translation="MKTSDLTYIARTFAQWFDSEHFDPAEINDVGDVDRINWLRTSPF
                     VILHVGCLAVFWVGWSPLAVQTALAMYLIRMFAVTGIYHRYFAHRTFRTSRWAQFAFA
                     VLGASSAQRGPLWWAAHHRNHHRESDLQADPHSPVQYGFLRSHAGWFMCNRYYATQYK
                     RIQDFARFPELVWLNRFDKAVPFALGFALYLTGAWLERSAPFLGVTGLQLFVWGFVVS
                     TTVLFHATASINSLAHLIGSRRYDTKDESRNNFLLALITLGEGWHNNHHQHMGCTRQG
                     FYWWEIDLTYYVLKLMSWTGLIWDLKPVPARAYNPSEQLPIKRIASTV"
     gene            complement(11692..12048)
                     /locus_tag="VF182_01425"
     CDS             complement(11692..12048)
                     /locus_tag="VF182_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435760.1"
                     /translation="MKAGRLSAAGESRSCPHCKATILKSSVSCPLCRHVLKFGSAAAE
                     AASSPTECPLSVEGTINHPGSGEALEYFILMEVRDESGKLVSRQSLGVGAIDKTQKRI
                     FTLRVEMSSTGMPPAA"
     gene            complement(12045..12548)
                     /locus_tag="VF182_01430"
     CDS             complement(12045..12548)
                     /locus_tag="VF182_01430"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX6435761.1"
                     /translation="MALWNQPAQEDNGEKPHVEHTAPIAPVAPSHAPREHTSKDRRES
                     VFGPGVAIEGKIEGDADLRIAGKFKGDIQVKGDVNIERGAHLSAKISAVTVTIAGEVE
                     GNVVASAQVKLLESGQLIGDLKATTLTVAAGSRMRGHVEFGWSEAQAAKFINGRAPEN
                     AKNGTNQ"
     gene            13106..14188
                     /locus_tag="VF182_01435"
     CDS             13106..14188
                     /locus_tag="VF182_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007260895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX6435762.1"
                     /translation="MTEVKATLHRVRVEVPLLSEQITTSVLFARVETDQGISGYGLTR
                     GTQRFALREFINRELAPFLRGKNPLETERIWQQLYATFNPRGQTGMWSSAVSAIDIAL
                     WDIKGKRYNEPVWRLLGGARNPVQSYVTFGLKQYSTEQLIEVAQQVVDQGEKRLKMVV
                     AVDPENPRVDAARVRAVRQAVGDEVELMIDANYLFSFNRALELCKLVEPYKITWFEEP
                     VYQNDALLLADLRRHTSIPIAAGQNEGHRYRHRELIVNRGVDIAQPNVCSVGGYTEAV
                     KVAALAQAFNLPIANGGGWPHHNMHLQAAMSNGWRVEFHFEMWGVGDKIYREPPAPDQ
                     GWITLPETPGLGLEPRWDALKDFEEK"
     gene            complement(14296..15498)
                     /locus_tag="VF182_01440"
     CDS             complement(14296..15498)
                     /locus_tag="VF182_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435763.1"
                     /translation="MGRAQKLIEENAKEGAVLNQRWYTLKRFGQYQDAIFSASHVRFY
                     GGLEKDVRAHLRRDYTSLVALEKDQEPGDIRLFSAKRESAPHDLVIIGQNGREVALKT
                     VVRLIYLAGFADAERQRKYRDALRSFTESLTVFLSSRSARQEFIGFFGKHGISSPDAV
                     LIGFIGETRALLKAQGIHDPELHSDESLRINWFKNANGRKVLLVSIDYNRIYASRAGD
                     LIEAIFAISGPSPPAIVFLGSGGAIEEPSIVGQIVTPTMVLNGDPLPLVQDKGVLVHL
                     IRNKAIGHASIKTAHASVENVVVETTRWAERMKSNRVRTVDQELFHIVKAVNSTANIQ
                     KFEMYVGILVTDNVSSNIDGNPDKTLEHAEETITKTADIRREFLKNILTELHILEPDE
                     IRRWQQRR"
     gene            complement(15711..16400)
                     /locus_tag="VF182_01445"
     CDS             complement(15711..16400)
                     /locus_tag="VF182_01445"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX6435764.1"
                     /translation="MMIIPASFFLMGSDEGPENERPRHQVWLDSFALAKFPVTNGEYK
                     IYVADSQAVPPPFWSERMFADPEQPVVGVSWHDAVAYCAWLRARTGKAFRLPSEAEWE
                     KGARGLRQNTLYPWGDESPCQRPYAGYDTLTGGPQRVGMNEPNDLGLYDMSEGVHEWC
                     SDYYDYRYYSYSPDKNPQGPPSGLRRASRGGSWRHRIKFSRCAARSSLPPSFRYADYG
                     FRLAWSVTESV"
     gene            16527..17570
                     /locus_tag="VF182_01450"
     CDS             16527..17570
                     /locus_tag="VF182_01450"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6435765.1"
                     /translation="MRISNIMAVALLTSISLFSGSAAHAQGTSFYKDKSIRIIVGFTA
                     GGLYDQYARILSRHMPKYIPGNPNIIVQNMPGAGSLTATNYVYSIAKPDGLTLGMIGS
                     GIYLDQMLGRKEASFDVTKLAWLGSVDQRDLVLYMKADSQWKSMDDILNISEPAKCGA
                     TGTSDLTTIMVNILEETLGAKFNNVRGYPGGVEIDLAIEKGEIQCRGTGITTHFAREP
                     YFTWHKSGFDRHLVQTGAKKDSRLPEAPTLSELMDKRKTSAISRNVARAMLVSATLGR
                     PLVGTPGIPPERIKVLREAYLKAFAEPEVVQEAKKNRLDLETLPGAEVETQIREVMNQ
                     PKEVIERVKKLSE"
     gene            17631..17888
                     /locus_tag="VF182_01455"
     CDS             17631..17888
                     /locus_tag="VF182_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435766.1"
                     /translation="MEIHIKMAPAQYKRLRNSYPIDSPVRDLIDNARRLDHAIEGVLF
                     AGYTIVCNEDQARIFLETARQCCPEVIPHIEQAIKLAQAGR"
     gene            complement(18009..18908)
                     /locus_tag="VF182_01460"
     CDS             complement(18009..18908)
                     /locus_tag="VF182_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HEX6435767.1"
                     /translation="MRRISHYHKIFLNVMPNATPAVIVHGGAGPIRDDSLPERLDGCK
                     AAALAAWDILAQNGSAVDAAEAAVMVLEDNPLFNAGTGSTLNQIGNVEMDAAIMDGDM
                     LRVGAVAALQRIKNPIKLARRVMEDGRHVILGGEGALKFAREIGFPECSPESLIVEHE
                     RKRWAEKHGTVGCVVLDANGRIAVATSTGGIFDKLPGRIGDSPLPGCGTYADDRGGVS
                     CTGLGEAIIRVVLGKTAVGFISEGVDPNEAARKALELLEAKTGGQAGLIMIDCRGRIG
                     YARNTTHMPVCYIKENQSVTIDS"
     gene            complement(18946..19572)
                     /locus_tag="VF182_01465"
     CDS             complement(18946..19572)
                     /locus_tag="VF182_01465"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX6435768.1"
                     /translation="MKLFDISLSLSPETIHWVTGTPLQLVERKRMSRGDTNNSSSIHT
                     SVHAGTHVDAPFHFVPEGVTIESLPVDLFIGPARVCELEPGSHITADDIDKLAIRGED
                     RVLFKTRNSQLLHKGVYDASFAAFSVDGAKALVDAGVRLVGLDYLSAAHADEQVPVHR
                     AFLDYGVVLLEGVDLSQVPPGQYELICPPVKLAGSDGAPCRAILRELN"
     gene            complement(19632..20507)
                     /locus_tag="VF182_01470"
     CDS             complement(19632..20507)
                     /locus_tag="VF182_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6435769.1"
                     /translation="MTSDGTAQLLALITSVLYASALVSARAGLRYSTPTTVTLFSILM
                     QNLLLWSAVFVSGGVHAVPMAGVLLFSLVGIFQLGVRLLAYTGVEKIGASRSSALQSI
                     SPLVSASIAVTILGEQPSLLIVLGTVLVVAGIVLVSWKPEQQLAAFRRWHLLLPLGAA
                     FLTGMNHPIRRYVLAMANEPLFFSALMGTVSLAGFSIYLAVSPQSQKLVWDRRAFWPF
                     MGTGLFETFSILCIITAISMGRVVVVAPIAASYPIWALIEARIFLRQMEPINPKTLLG
                     ILSVVAGNAAIHLGR"
     gene            complement(20785..21351)
                     /locus_tag="VF182_01475"
     CDS             complement(20785..21351)
                     /locus_tag="VF182_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013045210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6435770.1"
                     /translation="MMKRDEMLKAFARQRKDEIVVAVYMAAQEITHIAPNDLNYTFTG
                     AMGQGSSHALGLALGRPDRRIIVFDGDGSLLMNLGSLVTIANQAPKNLVHCLCENGTY
                     ETNGAVPIPRAGQIRFTDLAKTAGYRNTYELSDVAEWERELPRILNEDGPIFVDLKVE
                     PGEAYPENFPRLYSHEYRERFRRALAAS"
     gene            complement(21354..21863)
                     /locus_tag="VF182_01480"
     CDS             complement(21354..21863)
                     /locus_tag="VF182_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HEX6435771.1"
                     /translation="MADYPLTAAAIASEIKRAGIHFVVALPDRVTSQHLLKGMLSDGD
                     FHVVQVCKEDEGVSVCSGLYAAGKRSVLMMQYTGLLDSINSIRGIGVEGKFPVCMVVG
                     LLGKEPDVAPTKSKKYGVKIIEPILDVMGIEHHLVERDADAKKIGPAIDKAFAELFPV
                     AVLIGREPR"
     gene            complement(22003..23514)
                     /locus_tag="VF182_01485"
     CDS             complement(22003..23514)
                     /locus_tag="VF182_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6435772.1"
                     /translation="MPDKYDVIIVGGGSAGLVAANRLTEDPNRKVLLLEAGPDPQPLP
                     ELVAEASNQTRLLLESDYVAMFPTQRHLDGSTYYALAGRIMGGGSSVNVMSVLRPQRY
                     DLDQWVRAGNPDWSYETCLPFMKRIESDQDFPDSPIHGNDGPLYVKRHYTLDMPASPP
                     VRAFIDAAYSMGLPKCPDLNVPEPYGVCASPYNIKNGKRQSTTIAYLDPARGRPNLTI
                     IAEATVLSLQLSGKKADGVRYEKNGQVHLAQGGQILLTAGVYGSPQILMLSGIGQSDQ
                     LKKHGIPVVHELKGIGENYQDHPVVFMTFEGPKESKEDWVVPRFRLIIRKNPISEAAN
                     FHINMRPPTEVSGLKRMMPVSAHLLEQRNRGRVFLQSADPQEQLGIDSCMLEHPEDLK
                     AMIEAMRFIDELVHKEGTREFYGPLIQPGPGEDWGKFARSTYDSYHHGVGTCKMGPAS
                     DNMAVVDQRLRVHGIANLWVGDASVMPVVSRANTNLTSIMIGERLADFVQEVA"
     gene            complement(23539..23997)
                     /locus_tag="VF182_01490"
     CDS             complement(23539..23997)
                     /locus_tag="VF182_01490"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX6435773.1"
                     /translation="MIFEGKIDLDVPAVKTWDFLIDINKFSSCLPGLQEVKQIDDKTF
                     DGVIAANVGPISGKFSFRAMIVESNPPEQMMVKTEGTDSVTKSTVNADMLVDLQEPAA
                     NATMMNYRADIKIKGRLGILGDMVLRATATLILQEFTRRLREGLGATAKA"
     gene            24070..24279
                     /locus_tag="VF182_01495"
     CDS             24070..24279
                     /locus_tag="VF182_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435774.1"
                     /translation="MKAAQGKLNVSGRKAVCDIRDLNILPGLKLAFGSRKWDEQEPVN
                     VSQTCLHQAAKPSTMPTERWRTLDS"
     gene            complement(24326..26551)
                     /locus_tag="VF182_01500"
     CDS             complement(24326..26551)
                     /locus_tag="VF182_01500"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6435775.1"
                     /translation="MAASKQVVGNPTPRVEGELKVSGKAKYAVDVTLPGMLWGKLYRS
                     PIASGKITRIDVSKAEALPGVHAVVTGKDCTGLRIGRRLYDMPILADGEVRFIGEKVA
                     AVAADNESIAEEALNLIEVEYEETEPLLDPVDAMKSGAKLLHPDVMHYKGLPKALEAP
                     SNEFVYVTWKKGEIETGFRQADIIVENTFTTPVVHHAYIEPHSCVANVAPDGSAEIWA
                     CSKVPYAIREQVATALQIEQEKLVINPCFIGGDFGGKGDFMDVAVTYLLSKKAGQPVK
                     LVMDYDEELVAGNPRHASIIRVKTGVKKDGTIVAHHMDFVFDSGAYGAFKPNAFLNGP
                     HGSAGPYNIPHCLIEEHMVYTNKIPCGHMRSPGDPQGFFANESQMDLVAKRLGMDPLK
                     FRQKNLMHDGDVDPIGEEIAYIKTEETLAKALEDAGFNRPKPKNVGRGMAFVQWLANG
                     GFGTVALSLEEDGMVTISSAMLDQGAGTYTVLAEIVAEELKIPISQIRTQSLDTDEGT
                     KDSGVGGSRATRVYGNAGYEAALKAVEAIKQAAAEQLGTSPDQITLAKGAALHPRMER
                     RLSYSDLVKAKGAPIRVEATYNNNKKVHEASMCVQIAEVEVDPETGQVQLKKLTSTHN
                     TGTVLNPLMHQGQIEGGAMTGIGYALMEHVMINDGKVATANFGEYKIPTIADVPVFKS
                     SVSERPKGPGPYDSMPIGETSNVPTAAAIANAVEDACGVRIKSLPITAEKIYEALHGE
                     K"
     gene            27068..33025
                     /locus_tag="VF182_01505"
                     /pseudo
     CDS             27068..33025
                     /locus_tag="VF182_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018327319.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
     gene            33057..35174
                     /locus_tag="VF182_01510"
     CDS             33057..35174
                     /locus_tag="VF182_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6435776.1"
                     /translation="MNEATANILVVDDDIKTLKAMEALLAGPGRNISTASSGTEALRL
                     LLREEFALILMDVRLPVMDGFETAALIRQRERFRYTPIIFISAIDTLESDVFRGATSG
                     AVDYLFKPVVPQILQAKVSVFVDLFHKNEQLKLQAIRQSEERFRMVVESLQDYAVFMM
                     DPSGRVSTWNLGAERIVGWSQHEAIGKLFGAFYTPEDEEKGLPGQALKESAGAGRYED
                     EGWRRRKDGSRFWANVVVTALVDDHGELVGFSAIFRDLTARKKTEEQLKRLNSDLEER
                     YVEKAAELSHTIGEREKLQEQLLQVQKMESIGTLAGGIAHDINNILNIISGYANLIMQ
                     SSEGKDKNSEHVEVIIETVERGVSLVRQLLAMARKAETKFEHVRVTGVLQKLQGIIND
                     TFPKQIEVIWDLQPEMPSIMADPHQIHQAVFNLCLNARDAMPDGGKLIVSTEIVDGAE
                     LRKISQEAKEELYCCVSVKDTGVGMSLSTKNRIFEPFFTTKRQGEGTGLGLAVVYGIV
                     GKHGGIIDVESEPDRGASFRLFLPIPKNQTAASDPQAEQPRRKNEKVTADGELILFVD
                     DEVHQLRLMQIHLQRAGYKVLTAADGAEAVDMHYRHKHEIAAVILDFGLPKLNGWEAY
                     RKMKEADPDIKAIFATGFIASQLETELAKQELSGVIMKPYQLDEVLEKIDNAIKSKGV
                     KPVLDTRSIDDRPSFATTAKNRS"
     gene            complement(35413..35544)
                     /locus_tag="VF182_01515"
     CDS             complement(35413..35544)
                     /locus_tag="VF182_01515"
                     /inference="COORDINATES: protein motif:HMM:NF039620.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEX6435777.1"
                     /translation="MDFMLLGAISMASLIAGLFFLRFWKDTGDRFFLFFAVSFLIEA"
     gene            complement(35647..35817)
                     /locus_tag="VF182_01520"
     CDS             complement(35647..35817)
                     /locus_tag="VF182_01520"
                     /inference="COORDINATES: protein motif:HMM:NF039620.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEX6435778.1"
                     /translation="MIAGVFYTPCTVTAFFCFCLLLRAYYRSRYKLLLWGSVCFLGLT
                     LNNGLLATDKWL"
     gene            36473..37039
                     /locus_tag="VF182_01525"
     CDS             36473..37039
                     /locus_tag="VF182_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6435779.1"
                     /translation="MEPQTFYRKKHNSDLWHFCSNCTNWPIADFDATERGAPPSGDFC
                     GECIDKQHQGTCEQTIGAQPNAPIARKKILIIDDQPDVRRVLTISLRHLGYETCEASD
                     GSAGIRMSLMEHPDLIVMDLSLPDSSGLETAKRIKTNSQTSHIPIVACSGWKSDEILE
                     QAAEAGIVEFLGKPISPELLAEVIEKLT"
     gene            complement(37124..37199)
                     /locus_tag="VF182_01530"
     tRNA            complement(37124..37199)
                     /locus_tag="VF182_01530"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37164..37166),aa:Asn,seq:gtt)
     gene            complement(37391..37864)
                     /locus_tag="VF182_01535"
     CDS             complement(37391..37864)
                     /locus_tag="VF182_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HEX6435780.1"
                     /translation="MSNPTPLELRFAADVMLGRLAKWLRVLGQDVIYGRHLSGAGLIR
                     VARQENRMILTRDRRLRCQPQQSLFIQSDNFREQLHQVVSTFHLIRNNHLFKRCLICN
                     SSLQPRPKASVKGAVPEYVFASQEEFFWCADCRRIYWPATHHQKMLAELKNLQLD"
     gene            37923..39386
                     /locus_tag="VF182_01540"
     CDS             37923..39386
                     /locus_tag="VF182_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX6435781.1"
                     /translation="MISKQSIGLAIVAFWCVMNLLLIKRQLWAPPPPVFVRATEVLAE
                     GTNEWWGVYYHGEKIGFSSQRIEPNQNGYHLVDQSVLQLNLLGTTQRASTRLAMDVDR
                     EWVLKDFDFELLSNDVRFQSGGKVIAGQLHLEIESAGHTSKQTVALRQPPYLAAALKP
                     MVASQQLEPGKEHFFTIFDPATLAHHITSMVVEAREHILLGDQKVPAIRLRQKFKGIS
                     VVSWIDGNGRTLKEESPGGFALRQEAPSEAKSFADSRAVPLDLIARASITVGDSISEP
                     QTKRKLRLRLAGIDAAKFALDGERQKFSEGVLGIEREKVSPSDTYKLPMKNHRFTSFL
                     QPTAFLQTDHPSIRNHAARILGAETDAHRAVLRLKKWVYNEIKKQPTISIPNALQVLH
                     TKTGDCNEHTVLFNALARAAGIPARTVVGLVYLRGAFYYHAWSEVWLGKWVSLDSVLD
                     QFPADVTHVKFIEGEIDRQLDMLQLIGNLRIEVLGQE"
     gene            39502..40263
                     /locus_tag="VF182_01545"
     CDS             39502..40263
                     /locus_tag="VF182_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6435782.1"
                     /translation="MIRIINLRKQYGRLAAVDNLNIEVAPGEIFGFLGPNGAGKTTTI
                     RVMMGILRASSGRVILGGHDVAQEPQQAKAIAGFIPDRPFIYEKLSGKEFLTFIAKLH
                     RMESARLRRRIDELLEYFELANWQDELVEGFSHGMKQRLVLCAALVHEPRILIVDEPM
                     VGLDPKGARTIKDLFRSLAKAGTAVFLSTHSISVAEEVCHRIGIIQKGHLIASGTMAD
                     IYGLARGRDSNLEDVFLELTQQQDDLTRLPRERQL"
     gene            40260..41951
                     /locus_tag="VF182_01550"
     CDS             40260..41951
                     /locus_tag="VF182_01550"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435783.1"
                     /translation="MSTLLHLLSPKWLTWRNDFHRGRARSGRRWSLLALGMCFWIGTF
                     YVIRRVLIYFQSVHELGPALAYQLLLIILLTFLSMLLFSNLITALSAFFLARDLDLII
                     SMPLSQDGFYYSRLIMTAVNSSWMVLLFSLPIFAAYGTVFGGGFIFYLWLLLCLPLFL
                     IIPAAFGVLATHLFVYFLPAKRIRDILFFIGLFAFILIYFLFRFSQPERLVRPESFGH
                     FVQFLTAMETPSSPYLPSSWSAEILAGTLFKRSTDQAFFFALLASYGLFLPLVTSWVS
                     GAVYLDGRSKAQESNQGRRKLNWLDLLIDLLTRPFSAVMRAILIKDIKTFMRDSSQWS
                     QVFLLVALIVVYLYNFKVLPLDRSPMPAGTLRTIVAFVNLALAGFVLSAVAVRFAFPA
                     VSLEGNAFWLLQSSPISLRTLLWSKFWLNLLPLLFLGELLVFFSNLMLGVPNWMMVLS
                     LVTIFLMTVGITAICVGVGALYPKFAYDHVAEIPTSFGGAICMIFSIGFIGLIVMIEA
                     WPIYLLTSRSLALGGSHTPIEWLIAPSLISAIAVTVVAVVVPIRKSLRSLEAMGA"
     gene            42126..42809
                     /locus_tag="VF182_01555"
     CDS             42126..42809
                     /locus_tag="VF182_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6435784.1"
                     /translation="MELIHTLYEPAGKGPHPTILTLHGRGANALDLLGLAPHLCGGRL
                     LMICPQGPLETPIGPDASGYAWYPMSMGGPPDIASILMSRQKLQQFLDECLAHYPIDA
                     KKLAILGFSQGGVMAYSLALSAPDRFAALVALSSWLPPELVPHLSHGNSAQDLPTLVQ
                     HGTQDSMIEVERARQSVETLRQARIPLTYREYEMGHEIRPRSLSDLSAWLDEKVLSPI
                     ALANETRRD"
     gene            complement(42806..43684)
                     /gene="htpX"
                     /locus_tag="VF182_01560"
     CDS             complement(42806..43684)
                     /gene="htpX"
                     /locus_tag="VF182_01560"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX6435785.1"
                     /translation="MNMFRTTVLLALMTALLVWVGSVLGGQQGAVIAFVIAAAMNFFS
                     YWFSDKIVIKMYQGQEITPQDDPELYGIVQDLTQRAGLPMPKVYMLPQETPNAFATGR
                     NPQHAAVAVTAGIRRILDKRELAGVLGHELAHVSNRDILVSTIAATLAGAISYVAQMA
                     QWAAIFGGGHRDDREGGGNMISLLVMMIVAPLAALLIQMAVSRSREYGADVGGAKTTG
                     DPLALASALRKLHLGSQHIPLETSQATASATAHMFIVNPLTGGGFSALFSTHPPMEDR
                     IARLEAMARDHSYMRR"
     gene            43838..44707
                     /locus_tag="VF182_01565"
     CDS             43838..44707
                     /locus_tag="VF182_01565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX6435786.1"
                     /translation="MQIGVVFPQTEIGTDAGVIREFGQAAEQLGYHHILAYDHVLGAN
                     PASRPDWRPPYTHQSTFHEPFVLFGYLAALTKTIELVPGVLILPQRQTALVAKQAAAL
                     DVLSGGRLRLGIGIGWNPVEYEALGENFSNRGQRSEEQVQLLRLLWTREILSFDGRWH
                     KISDAGINPLPVQRPIPIWFGGGHDRVLRRLARMGDGWFPQLPPERCRAAIEKINAYA
                     REAGRDPETIGIEGRMTISAASAGTWNEQVKAWKEVGATYLGVNTMNSGFSNPAAHID
                     AIRNFREATKQLW"
     gene            complement(44721..45872)
                     /locus_tag="VF182_01570"
     CDS             complement(44721..45872)
                     /locus_tag="VF182_01570"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX6435787.1"
                     /translation="MIDCRRWGVLHRLQERVYPSRVMIRAFSCFLIVTLMLSSLLSGC
                     SPIYFLRAAYEEGRILWRRQPIADFLATPDLHYDTKEKLTTVLAVRDYAQNVLNLNVA
                     GSYASYSYVDRPDLTYIVIAAPQTELRPYTWWFLVVGHVPYKGFFSKSEAQSEAERLD
                     KQGYDTTIRTSAAFSTLGWFDDPLLSHLLRFDKIALSEIVFHELFHNTLYVKGAGAFN
                     ESAANFIGHRATIDFFGQRFGYESAEFRRAVQLWQEELEFAGFIEELIINLSDLYDRN
                     IAKEDKLRLRNEVFARSQSEWARRTATRPEHRFRGFSQQPLNNAVVMHYGVYLKNLQL
                     FESLYELEGGSLVRTINALEQSTQNGGEPFGALRSWMNKHKTGMIEQAG"
     gene            complement(45865..47682)
                     /locus_tag="VF182_01575"
     CDS             complement(45865..47682)
                     /locus_tag="VF182_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6435788.1"
                     /translation="MAEPSQQEEILGKAYDLRLIRRLWRYIRPYKRLFFFAMLLLPLQ
                     QAFGLAQPYLMKVGIDQYIAHGDLSGLQAVALLFLGAIVGETIMIFFHYYLTMAVAQR
                     CLADLRVDIFSHVQKLPMAYFDRNPVGRLVTRMTTDVDVLQEMFSSGVMTLISDFVMV
                     VWIIGIMFYLHAELTLVSLALIPPMALAINFFRIKARQTYRLIRERIARINAYLGEAL
                     SGMAIIQLFAREEKSYREFDDLNAAHRDAYHLSNLYEAALYSMVEAAGSVSVALLLWY
                     GGGEVVHGIIGIGTVVAFKEYIHRFFVPLRDFSQKYAVMQSAMSSAERIFQLLDTPVT
                     IDSPKKAVIPKPFRGEVVFDDVWFHYKADDPVLKGVSFRIEPGEKIAVVGATGSGKTT
                     TIKLLNRFYDVQKGSIQVSGVDVRDWDLQALRRHIGVVLQDVFLFSGDVRTNLALGDR
                     SVPLERIENAARLTNADIFIRRLGGGFSADVRERGSNFSAGQRQLLALARVLVFEPEI
                     LVMDEATSSVDTETEALIQDALERVMRDRTCLLIAHRLSTIRNADRIIVFHRGEVREF
                     GSHSQLMEKQGIYSRLYQLQYEREELRLPVQETEPKIYD"
     gene            complement(47683..49422)
                     /locus_tag="VF182_01580"
     CDS             complement(47683..49422)
                     /locus_tag="VF182_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6435789.1"
                     /translation="MGRLKTYLWRYWRRYLLGGLCLFGTATLVMWIPWWIREAVRILE
                     QGGALSDVTYYAAVIGLAALAQGIIRTCSRALIFNAGRNVEYDLRNDLFAHLQKLPLS
                     FYHARRTGDLMSRVINDISAVRVMLGPGVLNFANAPLYYVYGLALMLSMDVRMTLAAL
                     APFPLLMYAAAKFRGRIMKSSLEVQQQMSALSSHVQENLSGMHVVKAYAQEKYQTQQF
                     VALNEEFRIKSLEMARMRGIVTPLMQGINGLTVVIVIWYGGVRVARGDLLVADIVAFI
                     AYLNVLAWPTAAFGWMLSLVERGRAAMKRLEEVLNTQPAIASLPGALPLAELKKGIEF
                     REVSFAYEHQSNGHAILDNISFSLPIGKTVALVGRIGSGKSTLAQLVPRLFDASSGEV
                     LVDGQDVRKLSLRDLRKQLGYVPQEPFLFSTSLGRNLSFGRDDVSDQDLQRAVRIARL
                     ERDLENFPQGLDTVVGERGVTLSGGQKQRATLARALAIDPPVLILDDCLSSVDAQTEA
                     EILQGLRSILKEKTCLIISHRISAVKDADEILVFEDGRILERGSHHQLVERGGLYAEL
                     HQQQRLAEELEQI"
     gene            49535..51370
                     /gene="typA"
                     /locus_tag="VF182_01585"
     CDS             49535..51370
                     /gene="typA"
                     /locus_tag="VF182_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX6435790.1"
                     /translation="MTQTQNRRDDLRNIAIIAHVDHGKTTLLDAMLHQSGIFRANEHV
                     AERVMDSMDLERERGITIMAKNTTIHYGNIKINIVDTPGHADFGGEVERTLAMVDGVM
                     LLVDASEGPLPQTRFVLKKALESKLPPIICINKIDRPDARVAEVLDEIVDLFIDLDAT
                     EDQLDFPVVYTNARAGTATRDLKRPGETLKPLFELIVQALPGPNVDPAAVTQFQANNL
                     DYSDYVGRLAVGRVKNGTLTPGHYTLCRVDGKQELVKVTQVYGWQGLKRVEIPKTEGG
                     DIAAIAGIEDINIGDTITDRENPHPLPPLRIDEPTIAMVFNANNSPWSGREGEFVTSR
                     KLRERLFFEQRRNVSLRVEETDLPDAFRVTGRGEFALAIVIETMRREGYELQVSKPMV
                     VIREIDGVTHEPMELLVIDVPEDFIGVVSQLLAVRKGKMTKMIHAGSSRVRLEFAVPS
                     RGLIGFRSRFLTDTRGTGIMNALFNGWAPWHGHIQSRTNGAMVADREGPTTPYAVFHL
                     QERGVLFIAPGTKVYEGMIVGEYSRENDLNVNICREKKLTNIRAAGKDENILISPHRE
                     MGLEEGIEWIADDELVEVTPESIRLRKKILRQADRPRRRQEKDDE"
     gene            complement(51383..51745)
                     /locus_tag="VF182_01590"
     CDS             complement(51383..51745)
                     /locus_tag="VF182_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6435791.1"
                     /translation="MANQIVWCDIPVSELDRAVKFYSAVLGREVKRQDLPGMTIGVLP
                     HNDGEVGGCLLQSAEEKPSAQGVMIYLDANGRLDDAIAAVTAHGGKILQPKHAIGPFG
                     FRCIILDSEGNRLALHSD"
     gene            complement(51900..52142)
                     /locus_tag="VF182_01595"
     CDS             complement(51900..52142)
                     /locus_tag="VF182_01595"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX6435792.1"
                     /translation="MSHRLLLYSRAGCCLCEEMKTVIRRVAESTPLELDEIDIDGSAG
                     LKQKYGEEVPVLFINGRKAFKYRLTAKQLAARLKSS"
     gene            complement(52139..>54055)
                     /locus_tag="VF182_01600"
     CDS             complement(52139..>54055)
                     /locus_tag="VF182_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HEX6435793.1"
                     /translation="ASVQDGEVRIVKAGVDASLAQIRRWIDSGMGDGIWLEAELRNVA
                     IRLQGERALPLDAVQGRIVLNNDMITLSDISGNYGNSRLNSMAGTYSLRERALNLQAL
                     ADLDLAEVREQLLLGVFLTQAKILTDIQELNGRARASVTLNRIKEAPPQITGRLAFDN
                     AQVRKEDFLLNQGQGDIIFTGTEIKAEKIRAAISGSPVEIAFVVRDYASQDGVFDLSV
                     NSTGLKSGIVTRLLLSSGSVTDPGIVRGSVRYQGSLSNKDKRSFTGILDLVNVQLATP
                     PLLQPIRALNGNINIDESGIDFKNLRGLLVGFPSAANGRWRYAEQPQLVFDFTAPSLD
                     ITYLLTQLDAESSEFYANLQAQGRIAVANGKIKAIDFGDLKSDVTLDRRVWRLTNLTA
                     RAAGGAVQAKTTIDDRGDSLSIRADSQVRGVPVQSFLRWFDLTTTEMSGTVNLTGSLE
                     TAGNDDAERKRNMNGAFNLKIEDGIIHRMRILVQILNLLDLSRWFTLQLPDLAKDGIR
                     FRSIAGDFKVNKGVYYTQNLVVDSDDLRMTGTGRIDAPKDDIDFIVAVRPFAGIDTVI
                     NHIPLLGRGIAAIKNSFLVASFNIKGPIEEPTITPAPLGTLSEWFWGVLGIPKNAIGL
                     GENPKRDDNKAAPP"
BASE COUNT        12598 a        15046 c        14492 g        11919 t
ORIGIN      
        1 gtttttccga ccaaggccga tatccacacg ccggagatta cctcgagcca tctcaaaggc
       61 atggcgcatt acttcggcgc ggatttggtg ggcgttgtaa aattaccggc gaaaaagcgg
      121 tcaccctcgc ctctcccgca agcagccgac ggtaagatcg aagaagagta cccattcgca
      181 gtcgtctgca tagtgcgcgc tgagtaccat ccacgcagcg cccccggtat cggcgggcaa
      241 gtgcccgtgc agaacgggct gttcattact tttgtgctga gcgcgtggat aagggagctc
      301 ggctttcgcg ccaccgtcgc acaggaaact aatggcgagg acctcgctgc ggccgctgga
      361 ttgggcaaga tcaacggcaa cggccggtta gtgactcctg agtacggcgc cgatgttcat
      421 gtagccgata tcattctgac tgatctgcca ctggcgccag acggtgaaaa atgagtgcct
      481 ttcaagtagt tgaaaccctt cagtgcagaa aacgcgagtt gccccctaga cctccgatgg
      541 gctattcgta aaaactctat ctgaagttta ggtttcgctt ttgagcaagt tgcgggcacg
      601 ctctatgatt tcccaactac ttgaacattt tgatcggctt gaacgatctg aagtctgaga
      661 ggaacgattc agtgccctac gtgatcacca gtctatgcac caatgacgga gcgtgcgttg
      721 atgtctgtcc ggtcgcctgc attcatacga caccgggtgc gccgcaattc tacatcgatc
      781 ctgaagtgtg tatcgattgc gagcaatgcg aaatcgtctg cccggtggac gcaatcttca
      841 aagacatcga tatcccggcc gagcatcaag cggcgatcga agtgaacgcc gcctttttcc
      901 gtaagaacaa ggcggtcgcc ggtccggttt cattcgatac cgcttgggag atgatccgcg
      961 cggcgcatga ttatgcccag atcatgggat tgtcggtgac aaccgcggtg gtcgatgaag
     1021 cgggcgggcc gattgcgctg gggcgaatgg ataatgcgga tccggtgacg gcggaactag
     1081 cttttaacaa agcttacacc gccgccgcct ttcacgttgc caccgccgaa ttggcgccac
     1141 aagggcggca ggcgcgcttt cgcagcctga cgatatccca tcgcggtcgc attatgcccg
     1201 tgggaggagc tattccgatc atcgacggcg taaccattgt cggcgccgtc ggggtggccg
     1261 gcgcaagccg gcccgagcag gacattctct gttgccgtgc cgccctcgcc gttatcgaag
     1321 gccacggtca ttgaacacta acgagcatgg ccatgtacct cgttcaacta ctcttaccgc
     1381 tgtacgataa tttgaacgcc cgctttccgc ctgaggcttt cgatcgtgtg cggcaagagc
     1441 taaccgagca tttcggtggg gtcacggctt ttctgcgttc accagctcaa gggttctggc
     1501 ggatagctga gaacgcaatc aatcgcgatg aggtggttat cttcgaagtg atgaccgacg
     1561 aactaaaccg gcaatggtgg gatggatacc gagcggatct ggaacagcgc ttcaggcaaa
     1621 tcgaactggt gatccgggca agctcgatcg agaggctcta acggcagttt gttagagctg
     1681 gaacatctta cctgaatcat cagacacggc ttttcacctt ttttcgcttt cttgtcggcc
     1741 ctctcctccg ttaaagttcg gggacaacgc agatgccaaa ttggcttggc cggaaacagc
     1801 aatcctgcaa atctcgtctt accgttgcat taacagacgg ggattcatct cgaacgtgag
     1861 agacaccaaa tcaagcgagg gtaacgcttg aaccaaccgt ccatgcatca gtagaaacat
     1921 gggtatcacc acccatagaa accctaaacg gcagacaacc tcttggttcg gcatcgaaga
     1981 ccatttacta tcgtttacat gaaagaaatc cgctttgtcg acacgacgtt gcgcgacggc
     2041 cagcagagtc tttgggcgta cggcatgcgc actggaatga tcgttccaat cgccgagcac
     2101 atcgatgatg ccggctttga agcaatcgag ctcggcggtc ccgtggagct gccgaaatgc
     2161 gttcgcgaac tgcgcgaaga cccttgggag cgttaccggc tggtgttcgc tcgcatcaag
     2221 aaaacgccgc ttcgggtgat tcacgggacg cgtagtggtt tcgccatcta cccgcatgcc
     2281 gcacatcaac tgtacgatcg ctgcatgggc gatatcggga ttaagcaggt gcgtatctcg
     2341 gattcgtgga atgaccccgc cgactggcaa tggcgcgtca aacaagcgcg ggctgccgga
     2401 ctcgagccga tcatcaatct catctacaca atttcgccgc ggcacagcga cgaatactat
     2461 gcgcaaaaaa ctcgcgaagc actgaaactg aacgtattca gggtgtgctt gaaggatccc
     2521 ggcgggatcc ttacaccgga acgcacccgc accctggccc gcgcagttct ccaaagcgcc
     2581 aaagacacca tggcggagtt acacacgcac tgtaccaccg ggctcggttc gctttgctgt
     2641 ctggaagcga tcgaggcggg tatgacatcg atcaacacgg cgattcctcc gctggccgac
     2701 ggctcttcga acccgtcgat tttcaacgtt gccagaaacg cgaaggccat gggctacgcg
     2761 ccgatcatcg atgaagaacc cattcacgca gtggcggcgc atttcacggc catcgccaag
     2821 cgagagaagt tgaccatcgg caagccgccg gaatatgact acgcccaata catccaccaa
     2881 attcccggcg gcatgatttc aaatttgcgt caccagctgc gtctagtcgg catggaagac
     2941 aaactggagc ggaccctgga tgaagccgct caagtgcgag ctgaatttgg ctatccaatc
     3001 atggtcacgc cgctctccca gtttgtcggc agtcaagccg cgatcaatgt aatcgtcggc
     3061 gagcgataca aggaagtgac cgatcagacc atcgagtacg ccttgggtat ctggggaaag
     3121 gaaggcggcg cattgatgga tccggacata aaggacaaaa tcctcagccg gcgccgcgcc
     3181 gacgaaatcg gcaaacgtgc ggtgccgcag atgtcgctgc aggagttccg gcgaaaattt
     3241 ggcggatcag gcgtatccga tgaggaagtt ctaatgcgct tttttaccag caaggaagac
     3301 gtcgatacca tgcgcgcggc gggccctgcc cgcgtctacc ccaacggcgg aaatccgctt
     3361 ttgcatctgc tcgaagaact cacgagaaca accaatcgct ccagcatatc ggttcggcac
     3421 ggcgacgtct cggtgcattt gaaaagaaac acagcctcgt aaaatcttct acacgcaact
     3481 acaccgcgcg ggctcttcgt tatccgaacc gcctcgataa ttactccgcc ctgggacagg
     3541 ctcctataag aatgtgccgt cagcggcaca gcatcttctc atcgctaatc gctttggcaa
     3601 acacctgaga gcagttcatg gtgattcatt cttggcacag actttggttt gtagaagggc
     3661 caggagctac tgcaaggagg tatgtcatgg aaaagttcaa ggtttcttac tcgctcaaac
     3721 ttgggctggc attgggattg agcctgctgc tcggatgcgt ggcaggacca accttgcatg
     3781 aaacgcggcg tggcgagagt ctgccagggc cggacgcgac tttacagccc ggtgaaatta
     3841 gagccgaagt ggttcaggtt acgcccaatc gaaacgaaat ccaggttcga gccgatgata
     3901 atcgcagccg agtattgaga tacgatcccg gcagaacgcg ggtcacctat cagggccgcg
     3961 attactctgt cgagaatctt gaggccgggg atgttatcgc ctatcaaagt gccggtcgcg
     4021 atagcacaac aatcgatacg atccgcgtcc agcaacccgt tcaagcgcgt gcggcgtcaa
     4081 ctcccggccg gcccggatcg agtctaacca ggggtgaagt gctcgaaggc accgtcgata
     4141 gagtcgatta cgatcgcggc atcttcgatg tcaggacccg tgatcggggc acgatcacgg
     4201 tcgctttgcc tttcaacgcc cggcccagtg acgtagatgc tttccgccgc ctgcgcaacg
     4261 gcgacagcgt caggttggag ggagagttcg ttaatcgcga taacttccaa gtctcgtcga
     4321 tcagcaatcg ctagccgcta ggctggcgat tgctccgctg agatcatttc agcgcttttc
     4381 gcaatgcgcg gatcgcattg tcggcgtccc ttgcgttatc gaacgccagg taagtcacca
     4441 tccgccgtcc aagcgccgcg gccgatacgc cgcgcaggtt tattcctgca tcactgagcg
     4501 cgcgggtcag ttgagttccc aggccaggtt tgtccggtcc ttcaacccga accgaatgga
     4561 ggctatccgt ggtgctgagg ccggcgctct gagccgcatt cctctgcttg gttcctttta
     4621 ccggcgttag gaaaaccacc ccggtaccgg gacggtcggg cgcgcgacgc gaaatgagga
     4681 actccaaatt cgcgcctgca ttgctcaagg cctccaccgt cgcaccaagc ccacccggcc
     4741 gatcggcgat ctccccggcc catacatcta ctttcttgat gtcatatgcc atggtcaatc
     4801 tcctgtgatt tgtgtttgtc caaactagcc gctcatacca ccaggcgaaa caggctgtca
     4861 aattgcaact catcagcacc cgagtagaat ctcgtgaagg gcggccagca cgatttccta
     4921 gcgctgcact tcgagaactg tcgagctaag atctgtttgg tccgattaac aactggcaca
     4981 gcggccgaat atcgtctaga gcttccaaat gttcgacgta atcggtgatg cgttcgacag
     5041 atttgtcatc gagcgcttca cgggcgcaat cggcgaattt ctgtttgagt tcgtcgtcgg
     5101 tcattggagc ttcgcggctg cctttggcgt aatcgatctg ccgggaaaaa ctccggccat
     5161 cgcgcagacg aatggtaact ttgcatggac ggctacctaa agccgtcggt tttagatcgg
     5221 cctcaaggtg catctgaatc ttttcagcca gacgcaaaac attcttatct ttgactgcgg
     5281 aatcggtgaa tgcgtcaagg aagagccttc cgtcgatgat tgcgcgcgcc agcagataag
     5341 gcatggagaa tttcccctgc aaaccgtttt ccggcacgcg aaagacaatt ctttcaaacg
     5401 tatgccgcgc cacgccgaca tcgatggact cgatcatctc agcggtaatc ccatgcttgg
     5461 ttttgaactc gagcacgccg tctatggcgg gatgggtcag gccaccgcag ggataaggct
     5521 tgatcctaat gccgctgttg atcaattcat aaatcttccc caactcttcg agcgggcgct
     5581 tgtccggctc tgctccagga taaaacacct cgtaaaaacc gagtccggtt tcgattgcct
     5641 gattgttggc ggtgaaaccg gattgcgcca gcttggatgc caccactcca tttctcgcgc
     5701 caagcccgac gtgcaaaggt ttggacatcg tgccgaaatt acacaccaag ccactggcca
     5761 tcgaagccgc tgtgccgagc gtcatttcta cttgagattg gttgagcccc tgcagcttcg
     5821 cgcaggctac gccggcgccg aaggaaccaa aacttgaggg gggatgccag ccgtcctcgg
     5881 agcgctgccg cagcgacatg actagtttcg taatcgtctc gaaccctgcg acataagcct
     5941 caacgcaacg gcgcccgctg gcgccaagcg attctcctag agatataatc gccggaatga
     6001 tcggcgccgt gggctggccc atcagcgtaa agctgtgatc gaagtccagc gcatgcgccg
     6061 ctgtgccatt ggcgaaagcc gcatgaattg ccgtagactt aaatccctgt ccaatgacgc
     6121 tggactcttc ttttgcatgt tcctggcgaa cgatctcggc gcagatccgc gcgctcgatt
     6181 ctttgctgcc cgccaatgcc actccaaggc aatcgagcat cgctgttttc gccagcttca
     6241 ccgcttgcgc cggaatcttt tggaattcag tgtcgactac gaaccgggca atttgcgccg
     6301 tcgcctccat gacaccctcc gatttttgtt tgcttcctgc tgttttgccg acgtttattt
     6361 ggccataaat tttctgactt gctgcacgat ctcgggcggt tgatccaaaa cctccttgat
     6421 caaagcttcc aattcctcgc cggaagtgta atcgacttcc atcttggctt ttttggcgtc
     6481 ctctaacagc tctggatctt tcaagacctt gacgaaggat tcgcgcagaa tcttcactcg
     6541 ctccggcggc gtgcccggtg tcagcaaaaa cgggcggccg aactcaccgc cagacagcat
     6601 ggcttggacg atgcgacgat tgctggcggg aactttgaat tcctcgagga tctcgtagat
     6661 tgtcggcgct tccgccgcgc gcgtatcacg tttgcgtccg gtctgcagca aatggcgatc
     6721 gaaccccttc ttgtgccagc tattgaacgg ctcgcggccg aaatgggccg ccaaggtatg
     6781 agcgcggcac gcgatctcgc ctttctccac tgccaaatcc gcctcattgc cgccggggta
     6841 gcctaaaaca gaattgatgt tcgcgcccaa cgtcagttcc aaaattttag ccaccaggta
     6901 atcggaactg gtggtcccgg aaccgccgca gctgggcggc tctttcgctt tgacgatgtc
     6961 gccgacggtt ttgaacggcg tgtcggcgcg catgtagaag ataatcgaat cctgcgatgc
     7021 cgaaccgatc cagtgaaact ttcgcaagtc gaattgcact tcctttcgct cgacgagttg
     7081 ctcgatatag atgctgttgt tcggcaacac catgctcaag ccgtccggtt tagccacggc
     7141 gtacacgtag ttcgtggcgg tgagcgagcc cgcgcccggc atgttttgca cgatcacctc
     7201 gggattgccg ggaatttgtt tgccccaata gcgcgcgagc aggcgtcccc ataggtcata
     7261 aaaacttccc ggcgtcgatc ccaccataat gcggatggtt ttgcccttgt agaagtcagc
     7321 ctgtgcgaat accgcagagt caaacagtgg cagcacagcc aaggcgacgg ttaaaatgag
     7381 ccctgcaata actcttgaac ggtgacgcgt cacaaaacct ccacgaatcg ggagctgcgc
     7441 gagattattc gatggcgtga caatgtgtca aatgaagaac ttgttcctat ttagctgaca
     7501 tcatgagctt gcggccgccg gagttcatgc gtgcttgagc gaccgcgatc gatcctcatc
     7561 atgagagctc cgagttgcca cacacggtcc gaatccctgc ctgcttttac ggtttcattc
     7621 ccgaaaaggt attcaccgta gcgtgaagat tgcgagcgaa gtcggtggcg gcctgggtgt
     7681 tcatattgaa agcagaccgg ccatactcct tgaccgccat caaggcaggc agcggcgtct
     7741 gtttcagccg ctccagtaac ttgccgacgg cggagtcgag ttccgcggcg ggcacgacat
     7801 cgctgataat accaaacata agcgccttgt tggcatcgat ctcctgggtt gaatagatca
     7861 ggtaagttgc cgctttgcgc ggcacgcgat cgaccagcgc cgacatcgcg atggtcggca
     7921 tgatatggtg cgccatctcc ggtaactgaa aacgcgcctt atcgctggca acagtgatat
     7981 cgcacagcgc tgccagcgcg cagcccaacc cggcggcgcg cccttgaaca acgccgatga
     8041 tcggcacttt ggaacgacgg aacgattcat acagatcgaa gatcgtctcg gctctttcgc
     8101 gcatctgata agcttccagc gccggaccag gtttgcccat cgcttcacgt cccaggcaaa
     8161 agtcctcgcc ggcggctttg aatacgatca gccgcgactc ctttgcggca tcgtcaatca
     8221 tctgagcgag ctgcgccaag gtggcatcgt cttgccggtt gccgatgtca ggccggttca
     8281 aagtgagggt ggttacgtcc gcgtcttttc gcttgagaat gtagtctgcc atagtcagtg
     8341 cctccataac ttcaatgaac ccgactctag aagcctcaac tgcattttgt caacggaatc
     8401 ttccggtgat cccgatcatg aacacctgaa cgtttgcttc acgatcgaag cgatttgctc
     8461 gacctgatca aaactgacgc cgggctcgca aggcagctcg acgagatccg tccagatttt
     8521 ttccgcatgg ggaaacgacc gaaccgccag ctgctggtaa ggcgcaagga aatggatcgg
     8581 gatataactt ccttgaactt cgaacccggc accgcggagc gatgcgatca gatccgaggc
     8641 tggatcgtgc ccggtggact tgggcagcac tcgaatgacc tgagtcagac aggcggaacc
     8701 ttcccggtgg gacaccaaga gtaaatcttc gtttccaagc aatccgtgat acgcccgaac
     8761 gcgcgcccgg cgcgcggcca gattcgcttg caaacttttc atgagactcg aggcaaccgc
     8821 agctttgaga ttggacatgg attctttttg atatggcggc ggcagtgact caggatcagt
     8881 tggtgatgaa ccgtgtaagg cttcatgcag cggcactgac cagcgcctcc agcgatgcca
     8941 gatcagtgtc gatgaaagac ttcccagcgc gagagaaaga tcagcagctg ccaattcaat
     9001 ttcggcatct tggtcgaata cttctcggtg ccgcgatatg agcacaccgc cgccaagacc
     9061 ggagcaaact ttcgcagtgc caaaacttaa aatgccggca ttcccgaacg ttccgagaat
     9121 ccgcccatct aagctcgcgc ccagcgcctg cgcggcgtca tcgatgaccc ggatgtccgt
     9181 tccccgtgtt agatcgataa tggattttat ctccgcagga tttccaaaga gatgcggcac
     9241 gatgattgcc cgggttctct tggtcagcgc cggctcgacc gttctcgccg tcagattgag
     9301 ttcatctccg acatcggcca acacgggaca cgcgcccacc gctaagatcg gcgcgataac
     9361 gctgctgcag caaaacgtcg gcaccaccac ttcatcggct gatttcacgt cacaggatcg
     9421 caatgcaagt tcgagggcga ggcttcccga tccgcacaga atcgcatttt ggacgcccag
     9481 ttcttcgctg atgcaacctt tgagctcggt aagctcccgc ccagcgataa cccggcccga
     9541 gagtaagcaa cgaaggattg cccggaaggt tgcgccagtc cagaatggcc ttgcgagagc
     9601 tacgtgataa aaccggggaa gcaaagtcac gtgaagaatc tatcgaactg gatggctgcg
     9661 ggttcgaacc ggctcaacgg cggctagcgc taagaacaag cggatttcct gtgacgctga
     9721 ccaaataatc agcatccccg ccagcgccgg ccgacaaact tgtttaagtg cggcgccgca
     9781 gcaactccgc tagcagccgg caaaaactgt tcggctccga tttcgatagt aatgcgtgct
     9841 cgcgatcgag gttttcagcg catagtgtga cgcagattcc gacacgcgag tcagtgtttc
     9901 gattgactgc cggctttcgt cactgtgata tgtgacatcg gcgtatctgc attggcgcca
     9961 tgccaatgcc gttcacccgc cttgatgtgc acgacatcgc caactgtgat ctcacgctgc
    10021 tctttgtcgt ttgctacaga accgcgtccc gcagttatca ccaaaatctg atcgcaatcg
    10081 tgaacgtgcc accccgtcgt agcgccctgg cgaaagttga cgacattaca ccggaaatcc
    10141 tcagaatcag tttcaccaat aatcgcctgg cgggtacggc tcacatttcc tcccgaccat
    10201 ccgggaacag gtgttgccgt gtccatgggc ttttccgtta actctgaaat tttgagtaca
    10261 cgcattgacg cctccatata gtcgattagt tcgcataggc gaagcagcaa gtcacaacta
    10321 caccgccgct aactgattcg tcaagaaagc tatcggcaat tattcaagct gatcttctgc
    10381 tatgagtcgg ccccatctgc atgatgcaag cttattgctt cggccgttgc aggcgccaca
    10441 cctccgtcgc aacctaaaaa gatcgacaat tagcgacaat cccgtcgact acaagttttg
    10501 acatcacggc acgctggccg atcgttccgg gattctcgaa ttttttagaa ccatcctcat
    10561 tttcttagca aaagattgaa gctttcaccg gctcatgtta atagtgtccg gttaatgaaa
    10621 acgtcagatt taacttatat agctcgcacg ttcgcacaat ggtttgattc tgagcatttc
    10681 gacccagctg agattaacga tgttggcgat gtcgaccgga tcaactggct ccgcacctcg
    10741 ccgtttgtga tcttacacgt cggctgcttg gcggtgttct gggtcggctg gagccctctt
    10801 gcggtgcaga cggcgctggc catgtacctg attcggatgt tcgccgtaac cggcatttac
    10861 catcgctatt ttgcacatag aacgtttcgt acttcacgtt gggctcagtt cgcgtttgcg
    10921 gttctcggag cctcctccgc ccagcgcggt cctctttggt gggcagcgca tcaccgaaac
    10981 catcatcgcg agtcggactt gcaagccgat ccgcactcgc cggttcaata tggctttctc
    11041 cgctctcacg ctggctggtt tatgtgcaac cgctactatg cgactcaata caagaggatc
    11101 caggactttg cccgctttcc tgaactggtc tggttgaatc gcttcgacaa agccgtgcca
    11161 tttgcgttgg ggttcgctct ctacctgacc ggcgcttggt tggagcgatc agcgcccttc
    11221 ctgggtgtca ccggacttca attattcgtt tggggtttcg tggtcagcac cacggtgttg
    11281 tttcatgcca ccgccagcat caattccctc gcgcatctca tcggcagccg tcgatacgac
    11341 accaaagatg agagtcgcaa taacttctta ctggcgttga ttacactcgg ggaaggttgg
    11401 cacaacaacc atcatcagca tatgggttgt acccgacagg gtttctactg gtgggaaatc
    11461 gatttgacgt actatgtttt gaagcttatg tcgtggaccg gactgatctg ggatttaaag
    11521 ccggtaccgg ctagagctta caatccttca gaacagctac cgataaagag aatcgcttcg
    11581 accgtctgac ccagccgttt accaagccgg tcccaaatgt aactcgagag tttgaacgaa
    11641 attcagattg ccgcgatgaa ctagccgaac atggcgaaac atctcttgtg gtcaggcagc
    11701 cggcggcatg cctgtggaag acatctccac cctcagggta aaaattcgtt tttgcgtttt
    11761 atcgattgct ccgacaccaa gactctgccg cgaaaccaat tttccggatt cgtcacgaac
    11821 ttccatcaag ataaagtatt caagcgcctc gcctgatcct gggtgattga tggtgccttc
    11881 taccgagagc gggcactccg tgggactgga agccgcttct gccgcggcgg agccgaactt
    11941 gagcacgtgc cggcaaagcg ggcacgaaac cgagcttttc aggatcgtcg ctttgcaatg
    12001 cgggcagctg cgggattctc ccgcagctga aagacgtccc gctttcattg attggttcca
    12061 ttcttcgcat tctcgggagc ccggccatta ataaactttg ccgcctgtgc ctcgctccag
    12121 ccgaactcta catgaccgcg catccgcgaa ccggcggcta ctgtcaatgt cgtcgctttg
    12181 agatcgccga tcagctggcc ggattccagc agctttacct gcgcgctagc gaccacgttg
    12241 ccttctacct cgcccgctat cgtgacggtt accgcgctga ttttcgccga cagatgcgcg
    12301 cccctttcga tattcacgtc gcctttgact tggatatcac ctttgaactt gccggcaatg
    12361 cgcaagtcgg catcaccttc gatctttccc tcgatcgcga ccccgggtcc aaatacggat
    12421 tcgcgacgat ccttggaggt atgttcacgc ggcgcgtggc tcggtgctac cggagcgatg
    12481 ggcgcggtgt gctcgacatg cggcttctca ccattatctt cttgagcggg ttgattccaa
    12541 agggccatag aacctcctta atgtttgatg acagcggacg acaattatga actgaagctt
    12601 gagcgagaac ttttccaaac cgacgcaatg gaggtgccag ctgtcaattg caggacagtt
    12661 gggcgtcccg gaagcgcaga tcggccgtga actcggttca ggcgaggcac gcatggagac
    12721 ttttgggagc gtgaggtcgc gttgcctacg gaagctccat gcaaacccgt tcagggtgtc
    12781 agtaaaaacg tggcgcacgg aggttggcgg atgcttcaca aatgtcgccg gaaaactaga
    12841 tttgtacaca ggccgcgcgg catccgcttt ttgctaatta caagatttct aactatggat
    12901 cttcaatctt gcgaatgaga atttttcgat tctaaagccg cgcacaatcg cgcgaataga
    12961 tagttaatga aatcttgcgg tcgcgatcca ggtttgacaa ccaacgaaca gagctgtacc
    13021 gctagccgta tccgcgggat tgaatgtgga ttctcacgaa actttacggc tctgactcac
    13081 tatcgaagag gaggtcgatc tgaaaatcac cgaagtcaaa gcaaccttgc atagagttcg
    13141 tgtcgaagtg ccgttgctgt cggagcagat tactacttcg gtgcttttcg ccagggttga
    13201 aacggaccaa ggtataagcg gttacgggct tacacgcggt acgcaacgat ttgccctgag
    13261 agaattcatc aatcgcgagc tcgcgccgtt cctgcgtggt aaaaatccgt tggagaccga
    13321 acgaatctgg cagcagctgt acgccacctt taatccacgc gggcaaaccg gcatgtggag
    13381 ttcggcggta agcgccatcg atatcgcgct ttgggacatc aaagggaaaa ggtataacga
    13441 gccggtctgg cgcttgcttg gcggcgcccg taatccagtg cagtcgtacg tcaccttcgg
    13501 gctcaaacaa tacagcacag aacagttgat tgaggtcgcc caacaggtag ttgatcaagg
    13561 ggagaagcga ctgaaaatgg tcgttgccgt ggatccggaa aatcctcgtg tcgatgcggc
    13621 gcgcgtccgc gctgtgcgtc aggcggttgg cgatgaagtg gaattgatga tcgatgccaa
    13681 ttaccttttc tcatttaatc gcgcgctgga gctttgcaaa ctggttgagc cttataagat
    13741 cacctggttt gaagaaccgg tttatcagaa tgacgcgctg ctgctcgctg acttgcgccg
    13801 acatacatcg atcccgatcg ccgccggaca aaacgaaggt catcgatatc gtcatcgaga
    13861 actgattgtc aatcgtggtg tcgacatcgc gcagcccaac gtttgttcgg tgggcggtta
    13921 tactgaagca gtgaaggttg ctgcgctggc gcaagcgttc aatttaccca tcgccaacgg
    13981 cggtggttgg ccgcaccaca acatgcatct gcaggcggcg atgtcgaacg gctggcgagt
    14041 cgagtttcac ttcgaaatgt ggggtgtcgg cgacaagatc tacagagagc cgcctgcgcc
    14101 ggatcaaggc tggattacgc tgccggaaac acccggctta ggattggaac cgcgctggga
    14161 tgcgctgaaa gattttgaag aaaaatagcc ggagcccaac atacgctctg cggagccacg
    14221 tcacgcacgg cgatagggga tagttctcca gcgcttattt tatcgcagaa cgcgatgaac
    14281 gacaccggca gcagcctatc gcctctgctg ccatcttctg atctcatccg gctctaagat
    14341 atggagctcg gttaaaatat ttttcaagaa ctctctccgg atatcagcgg ttttcgtaat
    14401 tgtttcttca gcatgctcca gggtcttatc cgggtttcca tcgatgttgg aagagacgtt
    14461 atctgtgact aagattccta catacatttc gaacttctga atgttggctg ttgagttgac
    14521 cgccttcaca atatgaaaca actcctgatc aacagttctc actcgattgc ttttcatcct
    14581 ctcggcccat cgagttgtct cgaccacaac gttctcaacc gatgcgtggg ccgttttaat
    14641 gctggcatgg cctatcgctt tgttgcgaat cagatgtacc agaacaccct tgtcctggac
    14701 tagtggaagc ggatctccgt tcagcaccat cgtaggagtg actatctggc cgacaatact
    14761 cggttcctct atcgctcccc cgctgcccag aaaaacgatc gccggcggac tgggcccgga
    14821 gattgcaaag atcgcctcga tcaaatcgcc tgctcggctg gcatagatcc ggttgtaatc
    14881 gattgatacc aggagaacct tccggccatt ggcgtttttg aaccagttta tccgcaacga
    14941 ttcatctgaa tggagttccg gatcgtgaat cccttgagct ttcaacagcg cgcgagtctc
    15001 tccgataaat ccaattagaa ccgcgtcggg actgctaatg ccgtgcttac cgaaaaagcc
    15061 gatgaattct tgccgtgcgc tacgtgaaga gagaaagact gtgagtgatt ctgtgaacga
    15121 ccgcagcgca tcccggtatt tccgctggcg ctcggcatcg gcaaaaccgg cgaggtagat
    15181 caagcggacg accgtcttta gagcgacctc tctcccattc tgtccgataa tgacgagatc
    15241 atgcggcgct gattcgcgct ttgcggagaa cagccgaata tccccgggct cttgatcctt
    15301 ctccaacgca acaagagacg tgtagtccct tcgcaagtgt gctcgaacgt ccttttcgag
    15361 accgccatag aatcgaacgt gggatgcgct aaagatcgcg tcttgatatt ggccaaaacg
    15421 cttcagcgta taccatcgct gattcagcac cgctccttct tttgcattct cctcgataag
    15481 tttctgtgct cgtcccacga actcgtccgt tcgatcctcg aacgtaacat taggctcagt
    15541 gagagctgtg atggcatttg attgatcatc gcgagacgcg cgatgggtgt cgattcgcgc
    15601 cgaaccgcat gaaagcgaaa aaatgacaag gagcgcagtt aacgtagccg cgtaaggttt
    15661 catggtatgc gcgggccgcc gcttctctta tacaacgacg tccggcgagc tcaaacagac
    15721 tccgtgacgc tccacgccaa acgaaacccg taatcggcgt agcgaaaact cggcggcaga
    15781 gagctgcgcg ccgcacagcg gctaaacttg atgcggtgac gccatgaacc tccgcgcgac
    15841 gcgcgtctta accctgaggg aggcccctga ggattcttgt ccggagaata gctgtaatag
    15901 cgataatcat agtagtcgct gcaccactca tgaacgcctt cgctcatgtc gtataatccc
    15961 aaatcattgg gctcgttcat gccgacccgc tgcggtcctc cagtgagcgt atcgtaaccg
    16021 gcgtagggtc tttggcaagg tgattcatcg ccccaagggt acagcgtgtt ttgccgcagg
    16081 ccgcgcgcgc ccttttccca ttcggcttcg ctcggcaagc ggaaagcttt accggtcctc
    16141 gccctcaacc aagcgcagta agctaccgca tcatgccagc tgaccccaac caccggctgc
    16201 tcggggtctg caaacattcg ctccgaccaa aacggcggcg gcacagcctg gctatccgcg
    16261 acgtatattt tgtactctcc gttggtcacc ggaaattttg ccaatgcgaa cgaatcgagc
    16321 caaacctgat ggcgcggcct ctcgttttcc ggcccttcgt cgcttcccat caaaaagaag
    16381 cttgccggaa tgatcatcat ctcaggtagc gcctgaacca tcacgatcag attagatttg
    16441 cagaattggt gttgtcaatg aggtcatact tattgcacaa gcttcgcgga ttgcctataa
    16501 gcagttgtca aaggacgaaa gatggcatga gaattagcaa tataatggca gtggcgctgc
    16561 tcacttcgat ttctttattt tctggcagcg cggcacacgc tcaagggacg tcattctaca
    16621 aagataaatc catccgcatc attgtcggtt ttaccgcggg tggtctctac gatcagtacg
    16681 cgcgcattct ctcccgccat atgcccaaat acattccggg aaatccaaat atcatcgtac
    16741 agaatatgcc aggtgcgggt tccctgaccg cgaccaacta cgtttatagc atcgctaagc
    16801 ccgatggctt gacgttgggc atgataggga gcggcatcta tctcgaccag atgttgggcc
    16861 ggaaagaagc gagcttcgat gtcactaaat tagcttggct cggcagcgtc gatcaaagag
    16921 acctggtgct ttacatgaaa gccgactcgc agtggaaatc aatggacgac attctcaaca
    16981 tcagcgagcc tgccaaatgc ggcgcaaccg ggacatcaga tttgacgaca atcatggtca
    17041 atatccttga agagaccctg ggcgccaaat tcaataacgt tagaggctat ccgggtggcg
    17101 ttgaaatcga tttggcgatt gaaaaaggcg agatccaatg ccggggcacc ggcattacca
    17161 cccactttgc ccgggagccg tatttcacct ggcacaaatc cgggttcgac aggcacctag
    17221 tgcagaccgg agccaaaaaa gactcgagac tgcccgaggc gccgacgttg tccgaattaa
    17281 tggacaagag gaaaacgtct gccatcagcc gtaatgtcgc ccgcgcgatg ctggtctccg
    17341 ccactttggg acgtcccctg gttggtactc cgggcatccc gccggagcgg atcaaagttc
    17401 tgcgtgaagc ttatctcaag gctttcgccg aaccggaggt cgttcaggaa gccaagaaga
    17461 accggcttga tctcgaaact cttcccggcg ccgaagtcga aacgcagatt cgcgaggtga
    17521 tgaatcaacc taaggaagtc atcgaacgag tgaagaagct ttctgagtga ttgctagcta
    17581 tctaaaggct ggcaactatt tttgttaacg gcggaccgat gaggagcgcc atggaaatac
    17641 atatcaaaat ggctccggcg caatataagc ggttgcgtaa ctcctatccc atcgactctc
    17701 ccgtccgtga tctcattgac aatgccagac gtctggatca tgccatcgag ggcgtgctgt
    17761 ttgccggcta cacaattgtg tgtaatgagg accaagcgcg gattttttta gagaccgcgc
    17821 ggcaatgctg ccccgaggta atcccacaca tagagcaagc aatcaaactc gcccaagcgg
    17881 gccgttagaa atcgcattct aatcggctaa gctgcctagt tcgcccgaac agacggaaga
    17941 acacgaacag cgtccggatc catcagagac cgcccggcga agtcatgcga gtcaatgatt
    18001 agcggccctc agctatcgat ggttacgctc tggttctctt tgatatagca aacaggcata
    18061 tgggtcgtgt tgcgagcgta gccgattcgt ccgcggcaat cgatcatgat cagaccggct
    18121 tggcctcctg tttttgcctc caacagttcc agagctttcc gcgcggcctc atttggatcg
    18181 accccttccg agatgaagcc gacggcagtt ttgcccagga caacgcgaat aatcgcttca
    18241 cccaatcccg tgcacgaaac cccaccacga tcgtcagcgt atgttccgca gccgggcaac
    18301 ggcgaatcgc caatgcgacc cggcagctta tcgaaaatcc cgccagtgga agtagccaca
    18361 gcgatccttc cgttagcgtc caacactacg caccccaccg tgccgtgctt ctctgcccaa
    18421 cgttttctct cgtgttcgac aattagcgat tccggagaac attcgggaaa gccaatttcc
    18481 cgcgcaaact tgagggcgcc ctcgcccccc aggatcacat gccggccatc ctccataact
    18541 cgacgcgcca gctttatagg gttcttaatt cgttgtaacg ctgccacagc gccaacacgc
    18601 aacatatcgc cgtccatgat cgccgcatcc atctccacat tgccaatttg attgagcgtc
    18661 gaaccggtac cggcattgaa gagcggatta tcttcgagaa ccatcacagc ggcttccgcc
    18721 gcgtccactg ccgatccgtt ctgcgcaaga atatcccacg ccgccagcgc cgctgctttg
    18781 catccgtcga gccgctctgg cagcgaatcg tcccggatcg gaccggcgcc gccatgaacg
    18841 attacggctg gtgtggcatt gggcataacg ttcaaaaaaa tcttgtggta atgggagatc
    18901 ctacgcacga catcgatttt ttagcgttga aaatagcgcg cgttgtcaat tcagttctct
    18961 gaggatcgcg cggcagggcg cgccgtccga tcccgcgagt ttcaccggcg ggcaaataag
    19021 ctcatactgg ccgggcggaa cctgcgatag atcgacgccc tcgagaagca cgactccata
    19081 atcgaggaat gctcgatgaa ccggaacctg ttcatcggca tgagccgctg aaagatagtc
    19141 cagaccaacg agacgaacgc ctgcgtcgac caatgccttg gcgccgtcaa ctgaaaatgc
    19201 ggcgaaactc gcatcataga cgcccttgtg cagtagctgc gagttgcgcg ttttgaacaa
    19261 gactcggtcc tcgcctctta tcgcgagttt gtcgatatcg tctgctgtta tatgactgcc
    19321 gggttcgagc tcacaaaccc tggccggtcc aatgaataga tccaccggca gcgattcgat
    19381 cgtgaccccc tccggtacga aatggaaagg cgcgtcgaca tgggtcccgg catgcacgct
    19441 ggtgtggatc gacgatgaat tattcgtgtc accgcggctc atacgcttgc gctcgaccaa
    19501 ttgcagcggg gttccggtga cccagtgaat tgtctcaggg gaaaggctaa gagagatatc
    19561 gaagagtttc acggcaccac ctcctatcgt gcggatctta cgtgacacca tccacgctca
    19621 caagtatgaa ctcatctgcc cagatggatg gccgcattgc cggcaaccac gctcaatatg
    19681 ccgaggagag ttttcggatt gatcggttcc atctgtcgca gaaaaattcg tgcttcaatg
    19741 agcgcccaaa tcgggtagct ggcggcgatc ggcgcgacca ccacgacccg gcccatgctg
    19801 atggctgtga taatgcaaag aatcgagaag gtttcaaata gacccgtgcc catgaacggc
    19861 caaaaagccc tgcggtccca aaccagcttt tgcgactgtg gcgacaccgc aaggtagatc
    19921 gaaaatccag ctagagagac agtgcccatc agcgcggaaa aaaacagcgg ctcgttggcc
    19981 atggcgagaa cgtaacgacg aatcggatgg ttcatgccgg tcaggaaagc ggctccgagc
    20041 ggcagcaaca ggtgccagcg acgaaatgcc gcaagctgtt gctcgggctt ccaagaaacc
    20101 agcacgatgc cggcaaccac caacactgtc cccaaaacaa tcaaaagaga gggttgttcg
    20161 ccgagaatcg tcaccgcaat gctggcgctg accagcggac tgatcgactg aagcgcgctg
    20221 ctgcgcgaag cgccgatctt ttccacgccg gtgtaggcaa gaagtcgaac acccaattga
    20281 aaaattccca ccaaactgaa caaaagaact cctgccatgg gtaccgcatg cacaccgccg
    20341 ctgacaaaga ccgccgacca cagcagcagg ttctgcataa gaatcgaaaa cagggtcact
    20401 gtggtcgggg tcgagtaacg caggccggca cgcgcggaga ccagcgcgga agcgtatagc
    20461 accgaagtaa tcaaagccag cagttgcgcc gtgccgtcgg aggtcataac caggtgccat
    20521 tcgcgataac agaagatcgc cgtgatgaga ctgaaggcag tttgaattcc tcgagcctct
    20581 gctcgggccc gagactctta tattggttta aagatgcttt gaggcaaacg gttctctaga
    20641 agatctcggt agattcttcg tatgcggcaa ctcgattttt tccgaacagg cgactctggt
    20701 gaacgttccc ctggattgcg gccctaaacc ctcagaggca acagaatcca aggtaagcgc
    20761 ttggagcgca ttgaacagct cttttcagct ggctgcgagc gcgcggcgaa atcgttcacg
    20821 gtattcgtgg ctgtaaagcc gggggaaatt ctcggggtac gcttcgccgg gttcgacttt
    20881 caaatcgaca aagatcggcc cgtcttcgtt taagatgcgc ggcaactcgc gctcccattc
    20941 ggcaacatcg gacaattcgt aggtgtttcg gtatcctgcg gttttggcaa gatcggtaaa
    21001 tcgaatttgt ccggcgcgcg gtatcggcac agcgccgttg gtttcgtagg tgccgttttc
    21061 gcaaaggcaa tgaactagat tctttggcgc ttgattggcg atggtgacca agctgccgag
    21121 gttcatcagc aaactgccgt ctccatcgaa aacgatgatc cgccggtccg ggcgcccaag
    21181 ggccaaaccg agggcatggg atgaaccctg ccccatcgcg ccggtgaatg tataattaag
    21241 atcattgggc gcaatatgcg taatctcttg cgcggccatg tagaccgcca cgacgatttc
    21301 atcttttcgt tggcgcgcga aggctttgag catttcatcg cgtttcatca tcgctagcgc
    21361 ggctcccttc caattagaac ggctacagga aacaattcag cgaaggcttt gtcgatggcc
    21421 gggccgattt ttttcgcgtc ggcgtcgcgt tcaaccaggt ggtgttcgat tcccataacg
    21481 tcaaggatcg gctcgatgat cttcactcca tacttcttgg actttgtcgg cgccacgtcc
    21541 ggttctttgc ccagtagccc gacaaccatg cagaccggaa atttcccttc gacgccgatg
    21601 ccgcggatcg agttgatcga atcgagtaaa cccgtgtact gcatcatcag aaccgaacgc
    21661 ttgccagcgg cgtaaagccc gctgcacacc gagacacctt cgtcttcctt gcagacctga
    21721 acgacatgaa agtcaccgtc actcagcatg cctttgagaa gatgttgact ggtgacacgg
    21781 tccggtaagg cgaccacgaa gtgaatgccg gcgcgtttga tttcgctggc gatagctgca
    21841 gccgtcaatg ggtaatcggc catggagcct cccgggatag cgcttatgcg cgttcaaacc
    21901 gttcaagcag ttccagccgt tcaaaatctt cggaaaagat tttgatctga aacgatttga
    21961 acctttggaa cggcttgaac gttttgaacc aggtttttat tctcatgcga cttcctgaac
    22021 aaaatcggcc agtcgctcac cgatcatgat cgaggttaga ttcgtattgg cgcgactcac
    22081 caccggcatg accgaagcgt ctccaaccca aagattggca atcccatgaa cacgtaatct
    22141 ttgatcgacc accgccatgt tgtcggatgc tggtcccatc ttgcacgtgc cgacgccgtg
    22201 gtgatagctg tcgtaggtcg agcgcgcgaa cttgccccaa tcttcgccag gccctggttg
    22261 aatcaaagga ccgtagaatt cacgggtccc ttctttgtgg acaagctcat cgatgaaccg
    22321 catggcttcg atcatcgcct taagatcttc gggatgttcg agcatgcagg aatcaatgcc
    22381 gagctgctcc tgtgggtcgg cgctctgcag aaaaacccgg ccgcgatttc gctgctcgag
    22441 caagtgcgcg gaaaccggca tcatgcgctt aagaccgctg acctcggtcg gcggccgcat
    22501 gttgatgtga aaattggccg cctcactgat tgggttctta cggatgatca ggcggaagcg
    22561 cggaacgacc caatcttctt tggactcttt aggcccttcg aacgtcataa acaccaccgg
    22621 atggtcctga taattttcgc cgattccttt cagctcatgg accaccggaa tgccatgttt
    22681 ttttaactgg tcactttgac cgattcccga gagcatcaga atctgcggcg acccatacac
    22741 cccggcagtg agcaggattt gcccgccctg cgccaggtgc acctggccat tcttctcata
    22801 gcgcacgcca tcggctttct tgcccgagag ctgcagagaa aggactgtgg cctcagcgat
    22861 gatcgtcaaa ttgggccggc cccgcgccgg atcgagatag gcgatggtcg tcgactggcg
    22921 tttgccgttt ttaatattgt acggcgaagc gcaaacaccg tacggctccg gcacgttgag
    22981 atcaggacat ttcggcaagc ccatactata agcagcgtca atgaatgcgc ggaccggagg
    23041 cgacgccggc atatcgagcg tataatgccg cttgacatag agcgggccgt cattaccgtg
    23101 gatcggactg tcgggaaagt cctggtccga ttcaatccgt ttcatgaacg gcaagcaggt
    23161 ctcgtaggac caatcgggat tgcccgcccg cacccattga tcaagatcat agcgctgcgg
    23221 gcgcaacacc gacatcacat ttaccgaaga gccgccgccc ataatccggc cggcaagagc
    23281 atagtaagtg ctgccatcca ggtgccgctg cgtcggaaac atcgcgacgt aatctgattc
    23341 aagcaatagc cgcgtctgat tagaagcctc ggcgacaagc tctggtaggg gctgcgggtc
    23401 aggcccggct tccaacaata aaaccttgcg attcggatct tccgtaagcc ggtttgcggc
    23461 caccaagccg gcggagcctc cgccgacgat aataacgtca tacttatctg gcatcgaaac
    23521 tcttcctccg tcgcgcgctc atgctttagc tgtcgcgccc agaccctcgc gcaggcgcct
    23581 ggtaaactct tgcaatatca atgtcgctgt agcgcgtaaa accatatccc cgaggatccc
    23641 gagccgtcct ttgatcttga tgtcagcgcg gtagttcatc atcgttgcat ttgccgccgg
    23701 ctcttggaga tccacgagca tatccgcatt gaccgtgctc ttggtcaccg aatcggtgcc
    23761 ttcggttttc accatcattt gttccggcgg gttactttca acgatcattg cgcgaaaaga
    23821 aaacttgccc gagatcgggc caacattggc cgcaataacc ccgtcgaagg ttttgtcatc
    23881 gatttgtttg acttcctgca gtccgggcag acacgatgag aatttattga tgtcgatcag
    23941 gaagtcccac gtcttcaccg caggcacatc aagatcgatc tttccctcaa aaatcatcct
    24001 tgctctcaac cggaaacagc gccgatctta tgtgacaaaa aaactcctga caagagatga
    24061 aggcgaagaa tgaaagcggc acagggaaag ctaaatgtgt ccggccgaaa ggccgtatgc
    24121 gatatccgtg acttgaatat tctccctggt ttgaagctcg ccttcggttc ccggaaatgg
    24181 gacgagcagg agccggtcaa cgtgagtcag acctgcctac atcaggctgc taaaccatcc
    24241 acgatgccga cggagcgctg gcgcactctc gactcgtaaa cgatccagct ctccgaattc
    24301 gatgctccgg agaaccggta tgaaactact tctcgccgtg cagagcttcg taaatctttt
    24361 ctgcggtaat cggcaggctt ttgatgcgca caccgcacgc gtcttccacg gcgttggcaa
    24421 tggccgcggc agtgggaaca ttggatgttt caccgatcgg catgctgtcg taaggcccgg
    24481 gccccttggg tcgttcactc acggagctct taaacaccgg cacatcggcg atggtgggaa
    24541 tcttgtactc accaaaattt gcggttgcta ccttgccgtc attaatcatg acatgttcca
    24601 ttaatgcgta accgattcca gtcatggcgc cgccttcgat ctgcccttga tgcatcaacg
    24661 gattgagcac cgtgccggtg ttatgggtgg aagtaagttt cttcagctgc acctggccgg
    24721 tttccggatc gacctccact tcggcaatct gcacgcacat ggaagcttca tgaactttct
    24781 tgttgttgtt gtaagtcgct tccacccgaa tcggcgcgcc cttggctttg accagatcgc
    24841 tgtaggaaag ccggcgttcc atacgcgggt gcaaggcagc gcctttggcg agcgtgatct
    24901 gatccggcga tgttcccaac tgttcggcgg cggcctgctt aatcgcttcc actgctttga
    24961 gagcggcctc ataaccggca ttgccataga ctcgcgtggc ccggctacca ccgactccgc
    25021 tgtccttagt gccctcgtcg gtgtccaacg attgggttct gatctgactg ataggaattt
    25081 ttagctcttc cgcgacaatc tcggccagca cggtgtaggt gccggcgcct tgatcgagca
    25141 tggcggaaga aattgttacc ataccgtcct cttcgagact caaagcgacc gttccgaaac
    25201 cgccattggc gagccactgc acaaacgcca tgccacggcc gacgttcttc ggcttcggcc
    25261 gattaaaacc agcatcctcc agcgccttgg cgagcgtttc ttcggtcttg atgtaggcga
    25321 tttcctcgcc gatgggatcg acatcgccat catgcatgag attcttctgc cggaacttaa
    25381 ggggatccat gccgagcctt ttggcgacga ggtccatctg gctttcattg gcgaaaaatc
    25441 cctgcggatc gccgggagag cgcatgtggc cgcatggtat tttgttggtg tagaccatgt
    25501 gctcttcgat cagacagtgc gggatgttgt aagggccggc cgagccgtgc ggcccgttga
    25561 gaaaggcgtt cggtttaaac gcgccgtatg cgccgctatc gaaaacaaag tccatatggt
    25621 gcgcgacgat ggttccgtct tttttgacac cggtcttgac ccggatgatc gacgcatgtc
    25681 gaggatttcc ggcaaccagc tcctcgtcat agtccattac caacttcacc ggctggccgg
    25741 cttttttcga cagcagatag gtcacagcga catccataaa gtcgcctttg ccgccgaagt
    25801 ctccgccgat gaaacacgga ttgatcacca acttttcctg ctcgatctgc agcgcagtgg
    25861 cgacctgctc gcggattgca taaggaacct tgctgcacgc ccagatttct gccgagccat
    25921 ctggagctac attggctacg caggaatgag gttcgatata ggcatgatgc accaccggcg
    25981 tggtaaatgt gttctcaacg atgatatcgg cctggcgaaa accggtttct atctcgccct
    26041 ttttccaggt gacataaacg aactcgttag acggcgcctc tagcgccttc ggcagacctt
    26101 tgtagtgcat gacatccggg tgcaacaact ttgcgccgga cttcatggcg tcgacaggat
    26161 ccaaaagcgg ttccgtttcc tcgtattcga cttcgatgag attgagcgcc tcctcggcga
    26221 tgctctcatt atccgccgcc accgcggcaa ctttctcgcc gatgaaccgc acttcgccgt
    26281 ctgcgaggat cggcatatcg tagagccgcc ggccgatccg cagcccagtg caatcttttc
    26341 cggtcacgac ggcgtggaca ccagggagcg cttctgcctt gctgacatca atccttgtta
    26401 tcttcccgga tgcgatcggg cttctataga gcttgcccca caacattccc ggcagggtta
    26461 catcgaccgc atacttggcc ttgccgctga cctttaactc gccctccacc ctcggtgttg
    26521 gattgcccac aacctgcttc gacgctgcca ttaagctacc tccatcttct caaccaattg
    26581 atagtatcct aaaataattc ccgcacggcc gccgctgatg aacttgccta ggcattagaa
    26641 ggattagctg actgaggcgg gcgtggggtt attggaagaa tgtaggggaa aacagctcga
    26701 tctgcaacta cgtaaactca aagtgaactc aaatcttacg cgacttagcg atgtgtcgaa
    26761 aaagcaccag gacgagcgca gcaggcgttg gatgtgagca gctttgccgc ggtaagagag
    26821 aatcgtgatt gatgctccga tcgccctagg ggcgcccata actgtagccg ctggcgcgat
    26881 taatcgttgc accggctttc taattacaat aaccttgcgt ttcagccctt tgcctcaatc
    26941 aggacttttc atttcaacag gaacggcatt gccgaacact aacatttcag aattgtcctc
    27001 tgcccaaatt ccgcccgctt gacgtctcga ttatctatcg gcgcgccata cctacaggag
    27061 accgatgatg gctaaaagaa tggtttctcg ggatgaaagc cacgtgctgg attcgaaact
    27121 gctgctacag gtgcttacct cagtgaagaa gggcgatttt agcgcccggc ttcccggcaa
    27181 ctggactggg gtcgatggca agatcgccga taccatcaac gatatcatag agatgatgtc
    27241 ggatagcacc aaggaaatgg agcgagtcag ccgggtggta ggcaaagagg gacgattgtc
    27301 acaacgagcg ttgcttccag cagcagccgg ttcatggagg agcagggtca atgcggtgaa
    27361 ccaattgatt gatgacctgg tccgtccaac aaccgaaatg gcccgggtta tcggggccgt
    27421 ggccaagggc gatctaaccc agaccgtggc gctggacgcc gaaggccagc cgctgcgagg
    27481 cgaatttctc cgcaccgcta gaatggtcaa tgccatggtg aaacagctcg atggttttgc
    27541 cgctgaagtc acgcgtgtcg ctcgagaggt gggcactgaa ggcaaactcg ggggccaagc
    27601 tcaggttcga gatgtcagcg gggtatggaa agatttgacc gagagcgtga atatgatggc
    27661 cggtaatctg actgcgcagg tgcgcaatat cgccgacgtc accatcgccg tcgccaacgg
    27721 cgatctatcc aaaaaaatca ccgtcgatgt gcgcggcgag atcctacagc tcaaagacac
    27781 cataaacacc atggtggacc aactgagaag ctttgccgcc gaagtcaccc gggtggcgcg
    27841 cgaggtcggc accgacggca agctcggcgg ccaggccgtc gttcccggag tcgccggtac
    27901 ctggaaggac ttgaccgaca gcgtcaacgc catggcgagc aatttgacct cgcaggtgcg
    27961 gaatattgcc gatgtcacaa ttgcggtcgc caatggcgat ctatcgagaa aaatcaccgt
    28021 cgacgtcaaa ggagagattc tccagcttaa agacacgatt aacaccatgg tcgatcagtt
    28081 gcgcagcttt gctgccgaag tcacccgggt cgcgcgtgag gtcggcacgg aaggaaagct
    28141 gggcggccag gccgtcgttc ccggagtcgc cggcacctgg aaagacttga ccgacagcgt
    28201 caacttgatg gcgggcaatc tcacggacca ggttagaaac atcgctgatg tcaccatcgc
    28261 agtcgccaac ggcgacctgt ccaagaagat caccgtcgac gtgcgcggtg agattctcca
    28321 gctcaaagaa gccatcaata ccatggtcga tcagttgcgc agctttgccg ccgaagtcac
    28381 ccgggtcgcg cgtgaggtcg gcacagaagg aaagctgggc ggccaggcgc aggttcgcga
    28441 cgtcagcggc gtttggaaag atcttactga gaacgtcaac atcatggccg gaaacttgac
    28501 ggcccaggtg cgaaacatcg ccgacgtgac cattgccgtc gccaacggcg atttgtccaa
    28561 aaaaatcacc gtcgacgtgc acggtgagat tctccagctc aaagaagcca tcaataccat
    28621 ggtcgatcag ttaaacgcct tcgccgccga ggtgacacgc gttgcgcgcg aggtcggcac
    28681 cgatggcaag ttgggcggcc aggccgtcgt tcccggagtc gccggcacct ggaaggactt
    28741 gaccgacagc gtgaatttca tggccggcaa cttaaccgcg caggtgcgca atattgctga
    28801 tgtgaccatt gccgtcgcca acggcgactt gtccaaaaaa atcaccgtcg atgtgcacgg
    28861 tgagattctc cagcttaagg aagccatcaa taccatggtc gatcagctaa acgcctttgc
    28921 cgccgaagtg acacgcgtgg cgcgcgaggt cggcaccgat ggcaagttgg gtggccaggc
    28981 cttggttccc ggagtcgccg gcacctggaa ggatctgact gacagcgtca atgccatggc
    29041 gagcaacctg acctctcaag tgcgaaacat cgccgatgta accattgccg tagccaacgg
    29101 cgacttgtcc cgaaagataa ccgtcgatgt caaaggtgaa atccttcagc tcaaagacac
    29161 catcaatacc atggtcgatc agctaaacgc ttttgccgcc gaggtgacac gcgttgcgcg
    29221 cgaagtcggc accgagggta agctcggcgg tcaggctcag gttcgcgatg tcagcggcgt
    29281 ctggaaagat ttgaccgaca gcgtcaattt catggccggt aacttgaccg cgcaggtgcg
    29341 caatatcgcc gatgtcacca ttgccgtcgc caacggcgac ttgtcaaaga aaatcaccgt
    29401 cgatgtgcgc ggggaaatcc tccagctcaa agaagccatc aataccatgg tcgatcagct
    29461 aaacgctttt gccgccgagg tgacacgcgt tgcgcgcgag gtcggcaccg atggcaagct
    29521 tggcggacag gccctggttc ccggtgttgc cggcacctgg aaggacttga ctgatagcgt
    29581 caatttcatg gctggcaatt taaccgcgca ggtgaggaat atcgccgatg tcaccattgc
    29641 ggtggccaat ggcgatttgt ccaaaaagat taccgtcgac gtccaaggcg aaatcctcca
    29701 gcttaaagaa gccatcaaca ccatggtcga tcagctgcgc agcttcgccg ccgaggtcac
    29761 acgcgtcgcg cgtgaagtcg gcaccgaagg aaagctgggc gggcaggcct tggttcccgg
    29821 ggtcgccggt acctggaagg acctgactga taacgttaac gtcatggccg caaacctgac
    29881 tgcgcaagtg cgcggcatcg tcaaagtggt taccgccgtc gctaacggca gcctcaaaga
    29941 aaagcttaca gtcgaggcta agggtgaagt cgcggcgttg gccgaaacca tcaacaacat
    30001 gaccgatacc ttggccatct tcgcagaaca ggtcaccacc gtcgctcgcg aggtcggggt
    30061 tgaagggcgc ttgggcggac aggccaacgt gcccggcgct gccggcatct ggaaggattt
    30121 gaccgacaac gtgaacttgt tggcggcgaa tcttactact caggtacgcg ccattgccga
    30181 tgtcgccacc gcagtcacca agggagatct tacgcgttcg atccaggtcg cggcccgcgg
    30241 tgaggtcgcc gagctcaaag ataatatcaa tgcgatgatc cagaacctgc gcgcgaccac
    30301 cgagcagaac accgagcagg actggctcaa gaccaatctc gcgaaattca ctcgcatgat
    30361 gcaaggccag cgcgatctta cgaccgtggg ccagatgctg ctttcggagc ttgcgccgct
    30421 ggtcagcgcg cagtacggcg cgatttatca gatcgaagga agcaacggcg actcgcggct
    30481 gaaaatgctt gccagctatg cccgcgcggc gactcagaat gcggcagcgg aactgcccat
    30541 cggggtcggc ctggtgggtc agtgcgctat agagaaacgg cgcattttgc tctcggaagt
    30601 tccatctgat tacgttcaaa ttacttccgg cctcggccgg gcccgcccgc gcaacctgat
    30661 cgtgctgccg gttctgttcg aagcagagac caaagccgtc atcgagctgg cctcactcgc
    30721 tgaattcaca tcgacccatt tgacctttct cgcccagctc accgagagca tcggcatcgt
    30781 cctcaatacc atcgaggcaa cgatgcaaac cgaaggtctc ttgcagcaat cgcagaaact
    30841 tgccggcgaa ctacaaaccc agcagaagga attgcagcaa accaatgaag agttggcaac
    30901 caaagctcaa cagctggccg agcagaacgc cgaagtggag cgcaagaatc aggagattga
    30961 acaggcgcgt cacgctcttg aagagaaagc cgccgaactg gcgctggcgt caaaatacaa
    31021 atcggaattt ctcgccaaca tgtcgcacga attgcgcacg ccgctcaatt caattctcat
    31081 cctcgcgcag cagctgacat ccaatcccga gcggaatctt aacgcaaagc aggttgagtt
    31141 cgcccgtaac atccacgcct cgggcggcga cctgctgaac ttgatcagcg acattctcga
    31201 tctttccaaa atcgaatccg gcaccgtcag cgtcgaacct gaggagattc tcttcagcaa
    31261 agtgcaggaa gcggtggagc gaagcttccg gcatgtggcg gaagcgcgac accttacctt
    31321 caaaatcgaa agccataacg aactgcccca gggcatgacc accgatctca aacggctcca
    31381 gcaggtgctg aagaatctgc tctccaatgc gttcaaattc accacgcacg gaagcgttgt
    31441 cttccgcatg aaagtggctg aggcgggctg gagcccggaa cacccgatac tcaaaaacag
    31501 ttcgagggtg atcgcgttcg aagtcagcga cacgggtatc ggcattccgg ccgacaagca
    31561 gagaatcatc ttcgaggctt tccaccaagc cgatgccggc accagccgcc gttacggcgg
    31621 caccggtctt ggcttggcca tcagccgaga gctcgcgaca ttattgggcg gagagatccg
    31681 gcttatcagc aacgtcaacg agggcagtag cttcacgctg tatctgccgg aaaactattt
    31741 tgcctcagcg tcgggggact ttcccgcaag atgggctaca tttactccgg cgcccctggt
    31801 agtgcagcac tcgctcaagc ctgaagagat cgccgacgat cgcgaaacga ttttaccgaa
    31861 cgacgcggtg gtcctgatta ttgaagatga tccgcgcttt gccggactct tattggattt
    31921 ggtgcgcgag cgaggcttca aaggcgtagt taccaacaag ggatcaatcg cgggcaattt
    31981 ggcgcgcgaa ttcaatccag ttgccgtgct attggatatc ttcctgcccg acatgctcgg
    32041 atggacggtg ttgagcaatc taaaacaaga ctcggccacg cgccacatcc cggtgcaaat
    32101 cgtcagcgtc gaagaagaac gcatccaagg actgggtcac ggcgccttct cctatctgat
    32161 caaaccggcc accagcgggg agttggagat cgccattgac cgggtcttgg gctatgccag
    32221 ggcggaccgc aaggagctac taatcgtcga agacaacgcg gtggaaaggc aaagtatcgt
    32281 tgaactgctg tcccacgata aggtcgaaat cactgcggcg cacagcggcg gcgaggcttg
    32341 gaacctgttg cgctcaaaaa aattcgactg catggtactc gacttgaagc tgccggacat
    32401 caccggcttc gagttgctgg agcgcattcg ccgtgaacgg gatctgcgag atttgccgat
    32461 cgtcgtcttt accggcaaag acttaaccga ggaggatgaa ctgcaattgg cgcaaatggc
    32521 cgagagcgtg gtcgtcaagg gcgtccaatc tcccgagcgt ttgctcgatg aaacatcctt
    32581 gttcctgcac ctggtaacca ccgaactcgc gcctgccaag caacagatgc tcgaacggct
    32641 gcgccacagc gacgaggcgc tcagaggtaa gacggtgctg atcgtggatg atgacgtgcg
    32701 caatattttt gctttgacca gcttgctcga acagcacggg gtgcgcgtga tcagcgcgga
    32761 gaccggtaac gaggctattt cgctactcga tcgggaggaa gtgattgacg ccgtgctgat
    32821 ggatatcatg atgccggaca tggacggtta tgaaaccatg ggccttatcc ggaaaagcac
    32881 aaagcacagg ctactgccta ttctcgcgct cacagctaag gccatgaaag gcgaccggga
    32941 aaaatgcctg gaagcaggcg cttcggacta tatcgccaag ccggtcaaca cggaggaact
    33001 gctcggatta ctacgcatct ggcttttcgg caaacagcca cattctggag tcggctatga
    33061 acgaagcaac ggcgaacatc ctcgtagttg acgacgatat aaagacgctc aaggcgatgg
    33121 aggcgcttct cgccggcccg gggcgaaata tctctacggc ttcgtcgggt acggaggcgc
    33181 ttcgcctgtt actgcgagag gagtttgcgc tgattctgat ggacgtccgc ctaccagtga
    33241 tggacgggtt tgagactgcc gcgctaattc gacagagaga aaggtttcgc tacaccccga
    33301 tcatttttat ctctgccatc gataccttgg agtcggacgt ttttcgagga gccaccagcg
    33361 gggccgttga ctaccttttc aaaccggttg ttccgcaaat tcttcaggcc aaggtttcgg
    33421 ttttcgtcga cctgttccat aaaaacgagc agctaaaact gcaagcgatc cggcaaagcg
    33481 aggagcgctt tcgaatggtg gtcgaaagct tgcaggatta cgcggttttt atgatggatc
    33541 cgagcggccg ggtttcgacc tggaatctcg gcgccgagcg gattgtcggc tggagccagc
    33601 atgaagcgat tggcaaattg ttcggcgcct tctacacgcc ggaagacgag gaaaagggtc
    33661 tgcctggcca ggcattgaaa gagtcggcgg gcgcgggacg ctatgaagac gagggctggc
    33721 gccggcgcaa ggatgggtca cgcttttggg ctaatgtcgt ggtcaccgcg ctggtcgatg
    33781 atcacggcga gctcgtcggt ttttcagcta tcttccgtga tctcactgct cgcaagaaga
    33841 ccgaagaaca gcttaaacga ctcaactccg atctcgaaga acgctatgtg gagaaggcgg
    33901 ccgagctatc gcataccatc ggtgaacggg aaaagctgca agagcagctt ctccaagtcc
    33961 agaaaatgga aagcatcggt actttggccg gcggaattgc ccatgacatc aacaacatcc
    34021 tcaacatcat ctccggctac gccaatttga ttatgcaaag tagcgagggc aaagacaaaa
    34081 actccgagca tgtagaagtg attatagaaa cggtcgaacg aggcgtttct ttagtgcggc
    34141 agctcttggc catggcgcgc aaagctgaaa ccaaattcga acacgttcga gtcacaggtg
    34201 tcttgcagaa gctgcaaggc atcattaatg atacttttcc taaacagatc gaagtcatct
    34261 gggatctaca accggaaatg ccgtcgatca tggccgaccc tcatcaaatt catcaggcgg
    34321 ttttcaacct gtgcctgaat gcgcgcgatg ccatgccgga tggtggaaaa ctgatcgttt
    34381 ccaccgaaat agttgacgga gccgagctgc gtaagatttc tcaagaagcg aaagaggaac
    34441 tatactgctg cgtcagcgtg aaagacaccg gggtgggcat gagcctgtcg actaaaaatc
    34501 gcatttttga accgttcttt acgaccaaac gccagggaga gggaaccggt ttgggtctag
    34561 cagtagtcta cgggatcgtc ggtaaacacg gtggtattat cgatgttgaa agcgagccgg
    34621 atcgcggcgc aagctttcgc ctctttttgc cgattccgaa aaatcaaacc gcagcaagcg
    34681 acccccaagc ggaacagccg cgcaggaaaa atgagaaggt aacggccgac ggtgaattga
    34741 ttcttttcgt tgacgacgaa gtgcaccagc tacggttgat gcagatacac ctgcagcgag
    34801 cggggtacaa agtattgaca gcggccgatg gagctgaagc tgtcgatatg cactaccgac
    34861 acaagcatga gatcgcagca gtgatccttg atttcggatt gccgaagctg aacgggtggg
    34921 aagcttatcg aaaaatgaaa gaagccgatc ccgatatcaa ggcgattttt gccacgggat
    34981 ttatcgcttc tcagcttgaa acggaattgg ctaagcagga actgagcggc gttatcatga
    35041 agccctatca gctcgacgaa gtcttggaaa aaatcgacaa cgcgatcaaa agcaaaggtg
    35101 ttaaacctgt tctagatacc cgctcaatag atgatcgccc ttcgttcgcc acaaccgcca
    35161 aaaatcgttc ctgaccgcac acctcagact caggtgcagg atgcgctgac ctccaacatt
    35221 gagtcatgcg acagctcgtg ccgcttgggt tgtctcactg atggtcgata gacgaatctt
    35281 tcccggaaga actattgctc atgttttttt gaatgatcgc aatcaggatc aggagaaagg
    35341 agaggaatcg aacaaaatag aagaatgggc gggcctcgtt gggatcatca gagaatccca
    35401 gcgcggcgcg atttacgctt caatcaaaaa agaaacagca aagaaaagaa agaagcggtc
    35461 tcccgtatct ttccaaaagc gaagaaagaa caatcccgct atcagtgaag ccatggatat
    35521 agcgcccaat aacatgaaat ccatacgccg ctccctcaat ccatgtccaa ataattccaa
    35581 ataaaagaac catcatagca accagcgcaa cgaccaggcg ccaagtaaaa agatccacct
    35641 ccggacctaa agccatttat ctgtggctaa caatccgtta ttgagggtca aacccaagaa
    35701 gcacacactg ccccagagca gcaacttgta ccggctgcga taataggccc gcagcagcaa
    35761 acaaaaacaa aaaaatgcgg tcaccgtaca aggcgtatag aaaacaccgg cgatcattca
    35821 tttgtccttt ctaagtctaa aagcatcggc gaattcttga actctgtttt tgggcttggt
    35881 gtgaatcagg tttgtaatag gaccgagatg cttggcgtag attacggcta agaagtcgac
    35941 catttgttgc catgaggcgt cgccagctgg tatcgataca cagggtcctc ctcccgcgct
    36001 atcgtcacag agccatctgc atcgaggcgt gctagtaatt cggccgtttg ttttcaccga
    36061 tgtagaggcg gttggccaca gcttcacaac tccattacgc atctggtttg ctgcgaagaa
    36121 gcagcaacgc ttccagataa gcgaccgatt cgattctctc cggaataaat tgcttaactt
    36181 cgttcgggat cgggtcgcga tttatgcagt gatcggtgga tgaagttaat agcgtatgac
    36241 cggctgagtt gtaaacactg ggctggaaac aaagaacctg ggttcacttg atctcgagta
    36301 agcaattgaa ctctcttatg aagacaacat agcactgccc tgttcaagcg ttgcttgatt
    36361 gttacaggca ctaacacctt ttacaaacgg ccacgtcaaa ggtctcatga gggaaacttg
    36421 tcgacaacgg ttgtcaggat taccggaacg gcatttgcga atctggcggc cagtggaacc
    36481 gcaaactttt taccgcaaga aacacaatag cgatctttgg catttttgct ccaattgtac
    36541 gaattggccg attgcggatt ttgacgccac cgaacgcggt gctccaccgt ccggtgattt
    36601 ctgtggtgaa tgtatcgata agcaacatca aggaacgtgc gagcagacta taggagcaca
    36661 gccaaatgca ccgattgctc gcaagaagat cctgatcatc gacgatcaac ctgacgtccg
    36721 gcgggtgctt acgataagct tacgccatct aggctatgag acatgcgaag catccgacgg
    36781 cagcgccggc atccgaatga gcctaatgga acatcccgat ttgatcgtga tggatctttc
    36841 gttacccgac tccagcggtt tggaaaccgc caaacggatt aagacaaatt cgcagacgag
    36901 tcatattccg atcgttgcct gttccggttg gaagagcgat gagatccttg aacaagcggc
    36961 ggaagctggg atcgtagaat ttttaggcaa gccgatctcc ccggagctac ttgccgaagt
    37021 catcgaaaaa cttacctgaa ataagacacg gcatgatcaa tccgaacctg cacgcaaaac
    37081 gcccttcgac agatgtcgaa gggcgtttct cttaggtcta agctggctcc ccgggaggga
    37141 ttcgaacccc cgaccaattg gttaacagcc aactgctcta ccgactgagc taccggggaa
    37201 tagtagattg cttttcaccc gcccgaacac cgccacggcc gcgtctcatc gcaagctgtt
    37261 tgaaccggtt aaacccgggt gaacagatca tttaattagc acacgaatgc agcgaattcc
    37321 aggcaggaag actatcctgt gtccaaacct cgagagtgca aggcttcgca ggtcaatcca
    37381 acgaatgaga ttagtctaac tgcagatttt ttaattccgc cagcattttt tggtgatggg
    37441 ttgcgggcca gtagatacgg cggcaatcgg cgcaccaaaa aaattcctct tggcttgcaa
    37501 agacgtattc cggcaccgct cctttgacgg aagctttcgg tcgcggctgc agagaagaat
    37561 tgcaaataag acagcgcttg aagagatggt tgtttcgaat cagatgaaat gtgctgacca
    37621 cctgatgcag ctgttcgcga aaattatcgc tttgtataaa cagagactgc tgcggttggc
    37681 aacgcaatcg gcggtcacgg gtgagaatca tcctgttttc ttggcgtgcc acacggatta
    37741 aaccggcgcc tgagagatgc cgaccataaa tcacatcctg tcctaacacc ctgagccatt
    37801 tcgcaagcct cccgagcatg acgtcggcgg cgaatctcaa ttcaagcgga gttgggttgg
    37861 acatctgctg gcatttagtt gaactgattt gcctttccat ttaccatagc gtgttttacc
    37921 gcatgatctc taaacaatcc attggccttg ctatcgttgc gttctggtgc gtgatgaacc
    37981 tgttgctcat caaacgccag ctctgggcgc caccgccgcc agttttcgtc agagcgacgg
    38041 aagttcttgc agaagggacg aacgaatggt ggggagttta ctaccacggt gagaaaatcg
    38101 gctttagctc acagagaatt gaaccgaacc agaacggcta tcacttggtc gatcaatcgg
    38161 tgttgcaatt gaatctgtta ggaacgacgc aacgcgcttc gacccggctg gcgatggacg
    38221 tggatagaga atgggtgctt aaagatttcg attttgagct actatcaaac gacgtccgtt
    38281 tccaatccgg gggcaaggtg attgcaggcc agctacacct tgaaatcgag tcggccggcc
    38341 atacctcgaa gcagactgtc gcgttgcgcc aaccgcctta cttagccgca gctttgaaac
    38401 ctatggtggc aagccagcag ttggagcccg gcaaagaaca tttcttcaca atctttgacc
    38461 ctgcgactct ggcgcaccac atcacttcga tggttgtcga agcacgcgag cacattctcc
    38521 tcggcgacca aaaagtgccg gcgatccgat tgcgtcaaaa attcaaaggg atctctgtcg
    38581 tttcatggat cgatggaaac ggccgcacgc tgaaggaaga aagccccggc gggtttgctt
    38641 tacggcaaga agctccgtca gaagcgaaga gcttcgctga ttcccgcgcc gttcccttgg
    38701 atctgattgc acgggcttcg atcaccgttg gcgattcgat ttccgagcct caaacgaaac
    38761 gcaagttgcg actgagattg gcgggaatcg acgcggccaa gttcgcattg gatggggagc
    38821 gccagaagtt tagtgaaggt gtgttgggaa tcgagcgaga gaaagtaagt ccgtccgaca
    38881 cttacaaact cccgatgaag aaccatcggt tcacctcgtt tttacaaccc acggcatttc
    38941 tacagaccga tcatccatcg atccgcaacc atgccgcgcg aatcctcggc gcggaaaccg
    39001 acgcgcacag agccgtactg cgtctaaaga aatgggtcta caacgaaatc aaaaagcagc
    39061 ccaccatcag tatccctaac gctctccaag tactgcacac taaaacgggg gattgcaacg
    39121 aacatacggt gctattcaac gcgctcgcgc gcgccgcggg cattccagct agaacggtag
    39181 ttggcttggt ctatctgcgc ggcgcatttt actaccatgc gtggtccgaa gtgtggctcg
    39241 gcaaatgggt atcactagat tcggtgctcg accaatttcc cgctgatgtc actcacgtca
    39301 aattcattga aggcgaaatc gaccggcagt tagacatgct acagttgatc ggcaatctca
    39361 gaatagaggt tttgggacaa gaatgatttc aataaagtcg taggacgctt tcaaacctca
    39421 ttgcgcaaat gactccaaac caattaatcc gccgccttgt acggcatgga cttccgcaaa
    39481 aatagattcg acttgctcgc tatgattcgc ataatcaatt tacgcaagca gtatggccgc
    39541 ctggctgctg tagataatct caacatcgaa gttgctccgg gagaaatctt cggcttcttg
    39601 gggcccaacg gcgccggcaa gacgacgacc atccgggtga tgatgggtat cttgagagcg
    39661 agctcgggcc gagtgattct cggcggccac gatgtcgcac aagagccgca gcaagccaaa
    39721 gcgattgccg gattcatccc cgatcgcccg tttatttatg aaaaactcag cggcaaagaa
    39781 tttcttacgt tcatcgcgaa gctgcaccgc atggaatcgg ctcgattgag acgcaggatc
    39841 gacgagctat tggaatattt cgaattggcc aattggcagg acgagttggt ggaagggttt
    39901 tcacacggca tgaagcagcg cctggtcttg tgcgcggcgc tagttcatga gccgcgcatt
    39961 ctcattgtcg acgagccgat ggtcggcttg gatcccaagg gcgcgcgcac cattaaggac
    40021 ctatttcgtt cattggcgaa ggccggcacc gcggtttttt tatcgacaca tagcatcagc
    40081 gtggccgagg aagtatgcca ccgcatcgga attattcaga agggccacct gatagctagc
    40141 ggcacaatgg cggatatcta cggattggcc cgcggacgag acagtaactt ggaagatgtt
    40201 ttcctcgagc tgacccagca gcaggacgac ttaactcgat tgccccggga gcggcagcta
    40261 tgagcaccct tctgcacctc ctctcgccca agtggttgac ttggagaaac gactttcatc
    40321 gcggccgagc tcggtcgggc cggcgctggt cactcctggc cctggggatg tgtttctgga
    40381 tcggcacgtt ctatgtgatt cgtcgcgtgc tcatatattt tcaatcggtg catgagctcg
    40441 ggccggccct ggcctatcaa ctcttattga tcattctgct gacctttctg tcgatgctgc
    40501 tgttcagcaa tctcataaca gcgctctcgg cgttttttct cgcgcgcgat ctggacttga
    40561 tcatctctat gccgctttca caggatggtt tctattattc gcgtttgatt atgacggcag
    40621 ttaactcatc gtggatggtg ctacttttca gtttgccgat cttcgccgcc tacggcacgg
    40681 tcttcggcgg cggattcatc ttttacctgt ggttgttgtt gtgtctgccg ctgttcttga
    40741 ttattcccgc agcattcggg gttttggcta cgcacctctt cgtctatttt cttcccgcca
    40801 agcgcatccg cgatatactt tttttcatcg gcctgtttgc cttcattttg atttattttc
    40861 tatttcggtt ttcccagccg gaaagattag tgcggcccga aagcttcgga cacttcgtcc
    40921 aattcctaac cgccatggaa acaccatctt cgccttatct gccgagcagt tggtcggctg
    40981 aaatcctcgc tggaactcta tttaaacgct ccacggacca ggcgttcttc tttgcgttgc
    41041 tggcgagcta tgggttgttt ttaccgctcg taacgtcatg ggtttcgggc gcggtatacc
    41101 tggacggccg gtccaaggca caagaatcga accagggtcg gcgaaaactg aattggctcg
    41161 atctcttgat cgatctactg actcgtccgt tttcagcagt gatgcgagct attttgatca
    41221 aggacatcaa aactttcatg cgtgatagca gtcaatggtc tcaggtattc cttctagtcg
    41281 cactgattgt cgtctacctc tacaatttca aagtattgcc gctcgatcga tcgcccatgc
    41341 cggccggaac cttgagaacc atagttgcct tcgtcaactt ggccttggcc ggcttcgtcc
    41401 tcagcgcggt ggccgtgcgc tttgcgttcc cggcagtaag cctcgaaggc aatgcgtttt
    41461 ggctcttaca gagctcaccg atatcgctta ggacgctgct ctggagtaag ttttggctaa
    41521 acttattgcc acttcttttt ctcggtgaat tactggtctt tttcagcaat ctgatgctgg
    41581 gtgtgccgaa ctggatgatg gtcttatcgc tggtcacgat ctttctgatg acggtcggta
    41641 ttactgcgat ttgcgtgggc gtcggcgcac tctatcccaa attcgcctac gatcacgtgg
    41701 ccgaaatccc gaccagcttc ggcggtgcga tctgcatgat cttcagcatc ggcttcatcg
    41761 gcctcatagt tatgatagaa gcgtggccga tttatctact gaccagccga tcactcgcgc
    41821 ttggaggatc ccacactccg atagagtggt tgattgcccc ttctctgata agcgccatcg
    41881 ccgtgaccgt cgtcgccgtg gtagtgccga tcaggaaatc tcttagaagc ctggaagcta
    41941 tgggagccta ggagccgtag gttctgccgt agcgtgtccg gacagatcaa tttcggcatt
    42001 gaatttagaa atgaaccagt aacctcggcg cggtgaacaa aagctctggg catcaatgcg
    42061 catttgcaga acacggatgt ctcgataaac tatagccgat ggaagacaaa gctccgaggt
    42121 caccaatgga actgatccat acgctttacg agcccgcggg aaagggcccg catccaacta
    42181 ttctgacatt gcacggcaga ggcgccaacg ccctcgatct gctaggtctc gccccacatc
    42241 tctgcggcgg ccgattgctt atgatctgcc cgcaaggtcc tttggaaacc cctatcggac
    42301 cggacgccag cggttacgct tggtatccca tgagcatggg aggcccgccg gatatcgcgt
    42361 cgatcctaat gtcgcggcaa aagctacagc agtttctcga cgagtgtctt gcgcattatc
    42421 cgatcgacgc aaaaaaattg gcaatcctcg gctttagcca aggcggcgta atggcgtaca
    42481 gcctggctct ctcggccccg gatcgcttcg ccgccttggt cgcgctaagt agctggctgc
    42541 cgccggagct ggtgccgcac ttgtcgcacg gcaactcagc gcaggatttg ccgacgttgg
    42601 ttcaacacgg cactcaggat tcaatgatcg aggtcgaacg agcaaggcag tctgtggaga
    42661 cactgcgtca agcacggatt ccgctgacgt atagagaata cgagatgggc catgaaatca
    42721 ggccgagaag cctttcggac ctctcggcct ggttagatga gaaggtactc tcgccgatcg
    42781 cactggcgaa tgagacgcgc agagattaac ggcgcatgta gctgtgatcc cgggccatcg
    42841 cctcaagacg ggcgattcga tcttccatcg gcggatgcgt gctaaacagc gccgagaatc
    42901 ctccgccggt cagcgggtta acgataaaca tatgcgcggt cgcgctggcc gtagcctggc
    42961 tggtctcaag agggatgtgc tgtgagccga ggtgaagctt gcgcaacgcg ctggctaacg
    43021 cgagcggatc tccagtggtt tttgcgccgc caacatccgc tccgtactcc cgcgatcgcg
    43081 acaccgccat ttgaatcagc agcgccgcca acggcgcaac gatcatcatc accagcaggc
    43141 tgatcatatt accgccgccc tcacgatcat ctctatgacc gccgccaaag atagccgccc
    43201 attgggccat ctgcgccacg tagctgatgg cgccggccag ggttgcggcg atagtgctga
    43261 cgagaatgtc acgattggac acgtgcgcca gttcatgacc taaaacgccg gcaagctcgc
    43321 gcttatcgag aatgcgacgg atccccgccg tgaccgccac cgcggcatgc tgcggattac
    43381 gaccggtggc aaatgcgttc ggtgtctcct gcggcaacat ataaaccttg ggcatcggca
    43441 aaccagcccg ctgcgtgaga tcctggacaa tgccgtaaag ttccggatca tcctgcggcg
    43501 tgatctcttg gccttgatac attttgatta cgatcttatc actgaaccaa taactgaaaa
    43561 aattcatcgc agcagcgatg acaaaggcga ttactgctcc ctgttgaccg cctaggacgc
    43621 taccgaccca gaccagcagc gccgtcatca atgcgagtag aaccgttgta cgaaacatgt
    43681 tcataagttc ccctttgccg tatcgtacgg tttgattagg tgatgtcaag tacaaagcac
    43741 gcagtgtaat gtgcgtacga actagacaaa aggcaagagc cgagctagat aaaatcaaac
    43801 ggatcaatgg tttatcagtt cgggaacgga gaagacaatg caaatagggg tggtatttcc
    43861 gcaaacggaa atcggcacgg atgcaggtgt tatacgggag tttggacagg ctgctgaaca
    43921 gcttggctat caccatatcc tggcatacga ccatgtttta ggcgccaatc cggcgagccg
    43981 cccggattgg cgtccccctt acacgcacca aagcacgttt cacgaaccgt tcgttctgtt
    44041 cggttatctg gcagcattga ccaagacgat cgagttagtg cccggggttc ttattttgcc
    44101 gcagcgacaa accgccctgg tcgccaaaca ggcagcggct ctggacgttt tgagcggtgg
    44161 ccgcttgcgc ctcggtattg gcatcggttg gaatccggtt gaatatgagg cgctcggcga
    44221 aaattttagt aatcgcggtc agcgctcgga agaacaggtt caattgctgc gtctactctg
    44281 gacgcgcgag atcctcagct tcgatggtcg ttggcacaag atcagcgatg ccggaatcaa
    44341 tccactgccg gttcaacggc cgattccgat ctggttcggc ggtggtcacg accgggtgct
    44401 gcggcgttta gcccggatgg gtgacggctg gtttccccaa ctacctcccg agagatgccg
    44461 cgcggccatc gagaagatta acgcttatgc gcgtgaagct ggacgagatc cggaaactat
    44521 cggcattgag ggtcgaatga cgattagcgc cgcatcggcc gggacatgga atgaacaggt
    44581 caaagcctgg aaagaggtgg gggcaacgta tcttggcgtg aacaccatga actcgggatt
    44641 tagcaaccct gcggctcata tcgacgcgat ccggaatttt agggaagcaa caaaacagct
    44701 ttggtaagcg tttctctgcg tcacccggcc tgctcgatca ttcctgtctt atgcttgttc
    44761 atccaggaac gcagggcgcc aaaaggctcg ccgccatttt gcgtagattg ctctaaagca
    44821 ttgatcgtcc gcacgagact accaccttcg agctcatata aagattcaaa taactgcagg
    44881 ttctttagat aaacgccgta atgcatcacc actgcattat tgagcggctg ttgggaaaac
    44941 ccgcgaaaac gatgctccgg tcgggtcgca gtgcgtcggg cccattcgga ttgactgcgt
    45001 gcgaatactt cgttacgcaa acgcagctta tcttctttcg ctatgttgcg gtcatacaag
    45061 tctgaaagat tgatgatgag ttcttcgata aacccggcaa attcaagctc ttcctgccag
    45121 agttgaactg cccttcgaaa ttccgcgctt tcatagccga accgttgacc gaaaaaatcg
    45181 atggttgccc gatggccgat aaaattagcg gcactctcgt taaatgcgcc ggcacctttt
    45241 acatacaacg tattatgaaa gagctcgtga aaaactatct cgctgagcgc gatcttgtcg
    45301 aatcttaaaa gatgcgagag cagcggatcg tcaaaccacc cgagggtgct aaacgccgcc
    45361 gaagttcgaa tcgtcgtgtc atagccttgc ttatcaagcc gttcagcctc cgattgcgct
    45421 tcgcttttgg agaaaaaacc tttgtaaggc acgtgaccga caactagaaa ccaccaggta
    45481 tagggccgca gttcggtttg gggggcggcg atgacaatgt aagtcaaatc cgggcggtca
    45541 acataagagt aactggcgta actgccggcc acgttaagat tcagaacgtt ctgggcgtaa
    45601 tcgcgcactg ccaggacggt cgtgagcttt tctttggtat cgtaatgtag gtccggcgtg
    45661 gccaagaaat ccgcgattgg ctgccgccgc cacagtattc gtccttcctc gtaagcagcg
    45721 cgaagaaaat aaatcggcga acaacctgag agcaacgagc tcaacatcaa tgtcacgata
    45781 aggaagcagg aaaatgcccg gatcataacg cggctggggt aaacacgctc ttgcaggcga
    45841 tgcaaaactc cccatcgccg gcaatcaatc atagattttc ggctccgttt cttgaaccgg
    45901 cagtctcaac tcctcgcgct catattgcag ctgatataaa cgactataaa tcccttgctt
    45961 ctccatcagc tgtgaatgag aaccgaattc gcgcacttcg ccgcgatgaa acacgataat
    46021 gcggtcggca ttgcggatag tcgagagccg atgcgcaatc aataaacaag tgcgatcgcg
    46081 cataacccgc tccagggcat cctgtatcaa tgcttcggtc tcggtatcga cacttgaggt
    46141 cgcctcatcc atcaccagga tttccggctc gaataccagc acccgtgcga gtgcgagcag
    46201 ttgccgttgc ccagcggaaa aattgctgcc gcgctctcgc acgtcggcgc taaatcctcc
    46261 gccaagccgc cgtataaaga tatcggcatt ggtcaatcga gccgcgtttt caatccgctc
    46321 cagcggcacc gaacggtcgc cgagcgcgag attggtgcgg acatctccgg agaaaagaaa
    46381 cacgtcttgc aagacaacac caatatgtct tcgcagcgcc tgcaaatccc agtctcggac
    46441 atcgacgccg ctaacttgga tcgatccttt ctgtacgtca taaaaacgat tgagtagctt
    46501 gatagtggtt gttttccccg agcctgtcgc tccgacgacg gcgatttttt ctccgggttc
    46561 gatgcggaaa gacactccct tgaggaccgg gtcgtcggct ttatagtgaa accagacgtc
    46621 atcaaacacc acttcacccc ggaacggctt gggtatgacg gctttcttcg gactatcaat
    46681 cgtaaccggc gtatccagaa gctgaaaaat tctttccgcg gacgacatgg cagactgcat
    46741 gactgcatac ttctgactga aatcgcgcaa tggaacaaag aatcggtgga tgtattcctt
    46801 gaaagccact accgtgccga tcccgatgat gccatgcaca acttcgccgc ctccatacca
    46861 aagcagcaaa gccacgctca ccgaaccggc ggcttcgacc atcgagtaaa gcgcggcttc
    46921 ataaagattg gagagatgat aagcatcacg atgagcggca ttgagatcgt cgaactcgcg
    46981 atagctcttc tcctcacggg caaacagttg aatgatggcc atacctgaga gcgcctcgcc
    47041 caggtaggca ttgatccggg cgatccgctc gcgaatcaaa cggtatgtct ggcgcgcttt
    47101 gattcgaaaa aagtttatgg ccagagccat tggcgggatc agcgccaatg aaaccaacgt
    47161 caattccgca tgcagataga acatgatacc gatgatccat acaaccatga cgaaatcgga
    47221 gatcagggtc atcaccccgg aagaaaacat ctcctgtaga acatcgacgt ccgtcgtcat
    47281 gcgcgtgact aaccgtccga ccgggttgcg atcgaaataa gccatcggca acttctgcac
    47341 gtgtgagaat atgtctaccc ggaggtcggc cagacagcgc tgcgccacag ccatggtcag
    47401 gtagtaatga aaaaaaatca tgatggtctc gccgacgatg gctcccaaaa aaagcagggc
    47461 gacggcttgc aaacctgata aatcgccatg ggcaatgtat tgatcaatgc cgactttcat
    47521 caggtacggt tgcgccaggc cgaaggcttg ctgtagcggc aacagcaaca tggcgaagaa
    47581 aaacaggcgt ttgtaaggcc ttatgtatcg ccacagacgc cggatcagtc gcagatcgta
    47641 agccttgccg agaatttctt cctgctggga gggctcggcc acttagatct gctccaactc
    47701 ctcggccaag cgttgctgct gatggagttc cgcataaagg ccgccacgct ccaccagttg
    47761 atggtggcta ccccgttcga gaatgcgacc gtcctcaaaa acgaggattt cgtcggcatc
    47821 ctttacggcg gaaatccgat gggaaatgat cagacaggtt ttctctttca atatcgaccg
    47881 cagtccttgc agaatctcag cttcggtctg ggcatcaaca ctcgataagc agtcatcgag
    47941 aataagcacc ggaggatcga tggccagtgc gcgcgccaaa gtcgcccgct gtttctgacc
    48001 accggaaagc gtaacgccgc gttcaccgac cacggtgtcg agcccctgcg ggaaattttc
    48061 caggtcgcgt tcgagccggg caatgcgcac ggcccgttgc aaatcctggt cagaaacatc
    48121 gtctcggccg aagctaagat tgcgcccgag ggaagtcgaa aacagaaagg gctcttgagg
    48181 aacatagccc aattgtttgc gcagatcgcg cagcgacagc ttacgaacat cctgaccgtc
    48241 cacgagaacc tcgccggatg acgcgtcgaa cagccgcggc acgagctgcg ctagagtgct
    48301 ctttccggaa ccgatccggc cgaccagagc gacggttttt cctatcggca acgagaagct
    48361 gatgttgtcc agaatcgcgt gaccattcga ctggtgctcg taggcaaagg aaacctcgcg
    48421 gaactcgatt cccttcttta gctccgcaag cggcagcgcg ccaggcaaac tggcaatcgc
    48481 cggctgagtg ttgagaactt cctcgagccg cttcatcgcc gcacggccgc gctccaccag
    48541 cgacaacatc catccgaagg cggcagtcgg ccacgccaag acgtttagat aagcgatgaa
    48601 ggcgacgatg tccgccacca gtaaatcgcc ccgcgcgacg cgcacgccgc cataccagat
    48661 cacgatcaca actgtcagac cgttgatgcc ctgcatgagc ggtgtgacga tgccgcgcat
    48721 tcgcgccatc tccaaactct tgatgcgaaa ttcttcattg agtgcgacaa attgctgggt
    48781 ctgatacttt tcctgagcgt aggccttcac cacatgcatt ccactaagat tctcctgcac
    48841 atggctgctg agcgcggaca tctgctgctg aacctcgagt gaggacttca tgatccggcc
    48901 gcgaaacttg gccgcggcgt acataagcag tgggaacggt gccaacgccg ccaaggtcat
    48961 acgcacgtcc atcgagagca tcaaggcaag accatacaca taatagagcg gcgcgttggc
    49021 gaaattgagc actcccgggc cgagcatcac gcgcacggca gagatatcgt tgatcactcg
    49081 cgacatcaaa tcaccggtac gacgcgcgtg atagaacgac aagggcaatt tctgcagatg
    49141 ggcgaataga tcgttgcgca aatcatactc gacattgcgc ccggcattga agatcagcgc
    49201 ccgggagcag gtccgtatga tcccctgcgc cagcgcggcc agaccgatca cagcagcgta
    49261 ataggtgacg tcactgagcg cgccgccctg ctcgagaata cgcacggcct cgcggatcca
    49321 ccaaggaatc cacatcacca aggttgcggt gccaaacaag cacaacccgc ccaggagata
    49381 gcggcgccag taacgccaaa ggtaagtctt aagccgcccc atttaaatga tcaaaatact
    49441 ctagggggta agccttatca aggaattgtc gaagtcactg cggagtcgga ccattattgc
    49501 ttgacaatac caagtcgctc actaataaag atgtatgacg caaactcaga atcgtcgtga
    49561 tgaccttcgc aacatcgcta tcatcgctca cgtcgatcac ggcaaaacaa ctctcttaga
    49621 cgcgatgttg caccaaagcg gcatctttcg cgccaacgaa cacgtggccg agcgagtgat
    49681 ggactccatg gacctggaac gagaacgcgg catcaccatc atggccaaga acacgaccat
    49741 tcattacggc aatatcaaaa tcaacatcgt cgataccccg ggacatgccg acttcggcgg
    49801 cgaggtcgag cgcaccttgg ccatggtcga tggcgtgatg ctattggtcg atgcctccga
    49861 agggccgctg ccacaaaccc gtttcgtatt gaagaaggcg ctcgaatcca agttgccacc
    49921 aatcatctgc atcaataaga tcgatcgccc ggatgcgcgt gttgccgagg ttctcgatga
    49981 aattgtcgat ttgttcatcg acttggatgc aaccgaagat caactcgact ttcccgtggt
    50041 ctataccaat gctcgggcgg gcacggccac gcgcgatctg aagcggcccg gcgaaactct
    50101 gaaacctttg tttgagttga ttgtgcaagc attgccggga cccaacgtcg accccgcagc
    50161 cgtcacacag tttcaagcca acaacttaga ctatagcgac tatgtcgggc gactggcagt
    50221 aggcagggtc aaaaacggca cgttgactcc cggtcattat acgctgtgcc gggtagacgg
    50281 caagcaagaa ctcgtcaagg taactcaggt ttatggctgg caaggattga aacgggtcga
    50341 gattccaaaa actgaagggg gcgacatcgc tgccatcgcc ggcattgaag acatcaatat
    50401 cggtgacacg atcaccgatc gcgaaaaccc ccacccctta ccgccgctgc gtatcgatga
    50461 gccgaccatc gccatggttt tcaacgccaa caattcgccc tggtcgggcc gggaaggcga
    50521 gttcgtgacg tcgcgcaagt tgcgcgaacg gcttttcttc gagcaacgcc gcaacgtcag
    50581 cctgcgcgtc gaggaaacag acctacccga tgcgttcaga gttaccggcc gcggtgaatt
    50641 tgccctagcc atcgtcatcg aaacgatgcg tcgtgaaggt tacgagcttc aggtctcgaa
    50701 acctatggtg gtcattcgag aaatcgatgg cgtcacccat gaaccgatgg aactgctggt
    50761 catcgacgtg cccgaggatt ttatcggcgt cgtatcgcag ttgctggctg tccgaaaagg
    50821 caagatgacc aaaatgatcc atgccggatc gagccgggtg cgtttggaat tcgccgttcc
    50881 gtcgcgcggc ctcatcggtt ttcgctctcg ttttttgacc gacactcgcg gcaccggtat
    50941 tatgaacgcg ctcttcaatg gctgggcgcc atggcacggc cacattcaat cgcgaaccaa
    51001 cggcgccatg gtagcggacc gcgaaggtcc aacgactccc tacgctgttt ttcaccttca
    51061 ggagcgtggc gtgctgttta ttgcgcccgg cacgaaggtt tacgaaggaa tgatcgtcgg
    51121 cgagtattca cgggagaacg acctcaatgt gaatatctgc cgggaaaaaa agctcacgaa
    51181 cattcgagcc gccggcaaag acgaaaacat cctgatctcg ccgcaccgcg aaatgggttt
    51241 ggaagaagga atcgaatgga tcgccgatga tgaactggtc gaagtgacac cggaatcgat
    51301 ccgcctgcgc aagaagatcc tcagacaggc cgatcgaccc aggcgccgac aggagaagga
    51361 cgacgagtga agccgccgta gttcagtcgg agtgtaaggc cagccggttt ccctcgctat
    51421 ccaagatgat acaacgaaat ccgaacggtc cgatggcatg ttttggctgg agaattttgc
    51481 cgccgtgagc agtcactgct gcaatcgcat cgtccaatcg cccgttggca tcgaggtaga
    51541 tcatgacacc ttgggcggac ggcttctctt ccgcactttg caataagcac ccacccacct
    51601 caccgtcgtt atgcggcagc acaccaatgg tcattccggg caagtcctgc cttttgactt
    51661 ctctgccgag tacagcggag tagaatttta cggctcggtc cagctcggaa actggaatat
    51721 cacaccaaac aatttgattg gccatgagct cttcctttga agaatctata attcaagtcg
    51781 gtttcgtgaa gtgcgttact ttctttttcg ttctaaatca cttatagcct gcgctcgcaa
    51841 gagatgaaag cctgcagccc atttgtcgag atcgcaaaac acttaaactc caggagcgac
    51901 tagctggact tcaatcgggc agctagttgc ttcgcggtca gccggtattt gaatgctttg
    51961 cggccgttga tgaacaacac aggaacctct tccccgtact tttgtttgag accagcggag
    52021 ccatcgatgt cgatttcgtc aagctcgagc ggagtgcttt cggctacacg tcgaattaca
    52081 gttttcatct cctcacacag acaacagccg gctcgagagt acaagagcaa tcgatggctc
    52141 atggtggcgc cgccttgtta tcatctcgct tggggttctc acccaagcca atcgcgttct
    52201 tcggtatgcc gagaaccccc caaaaccatt ctgataaggt gccgagcggc gccggcgtaa
    52261 tcgtgggctc ctcaatcgga cctttgatat taaagctcgc cactaagaac gagttcttta
    52321 tcgcggcgat accacggcca agaaggggta tgtgattgat cacggtatcg atccctgcaa
    52381 acggtcgcac ggcgacgatg aaatcgatgt cgtctttcgg cgcgtcaatt ctcccggtgc
    52441 cggtcatgcg cagatcatcg ctatcgacca ccagattttg ggtgtaataa acccctttgt
    52501 tcactttgaa atcgccggcg atggagcgaa agcggatacc atccttggcc aagtcgggaa
    52561 gttgcaaggt aaaccatcgg gataagtcaa gtagatttaa aatctgcacc agaatccgca
    52621 tacggtggat aatcccgtcc tcgatcttga gattgaaagc gccgttcata ttccttttac
    52681 gttcggcatc gtcgttgccg gcggtctcaa ggcttccagt taagtttacc gttccgctca
    52741 tttcggtcgt ggtcagatcg aaccaacgga gaaacgactg aaccgggact cctcgaacct
    52801 gagaatccgc acgtatgctc aagctatcgc cacgatcatc aatggtcgtc ttagcctgca
    52861 cggcgccccc cgctgcgcgc gctgtaagat ttgtcagacg ccacacccgg cggtcaaggg
    52921 tgacatcact ctttaaatca ccgaagtcga tcgccttgat ctttccgttc gcgacagcga
    52981 tgcgaccttg agcctgcaga ttagcgtaga actcactcga ttcggcatcg agctgtgtca
    53041 gcaagtaagt gatgtccaag cttggcgcag tgaagtcgaa gaccagctgc ggttgctcgg
    53101 cgtagcgcca gcgcccgttg gcagcgctgg gaaatccaac gagcaagccc cggaggttct
    53161 taaaatcgat tccgctctca tcgatgttta tgtttccatt caaggcgcga atcggctgca
    53221 ataacggtgg agtggcgagc tgaacgttaa ccaaatccaa aatgccggta aagctccgtt
    53281 tgtccttgtt gctcaacgat ccttggtatc gcaccgaacc gcggacgata ccgggatcag
    53341 tgaccgatcc cgacgagagc aagagccgcg tcactatacc ggatttcaag cccgtcgagt
    53401 tgacgctgag atcgaaaacg ccgtcttgtg aggcgtaatc cctcactaca aaagcgatct
    53461 cgaccggcga tccggatatc gccgcgcgga ttttttcggc ttttatctct gttccggtga
    53521 aaatgatgtc gccctgcccc tgattcagaa gaaagtcttc cttgcgcacc tgcgcattat
    53581 cgaaggcgag cctgccggtg atttgtggcg gcgcctcttt gatacggttt aaagttaccg
    53641 aagctcgagc tctgccgttg agttcttgaa tatccgtaag gatcttcgcc tgcgtcagaa
    53701 agacacctaa caacagttgc tcccgaactt ccgcaagatc caggtcggcg agagcttgca
    53761 agttaagcgc acgctcgcgc agtgagtacg tcccagccat gctgtttaat cgcgaattac
    53821 cgtaatttcc gctgatatcg ctaagggtaa tcatatcgtt gtttagaact atccggcctt
    53881 gaaccgcgtc caggggcaga gctcgttcac cttggagcct gatggcgacg ttgcgcagct
    53941 ccgcctctaa ccagatgcca tcacccatgc cggagtctat ccaacggcgt atctgcgcaa
    54001 ggctcgcatc gacaccggcc ttcacgatac gcacctcccc atcttgaacg cttgc
//
LOCUS       DASUVS010000027         6336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_663613, whole genome shotgun sequence.
ACCESSION   DASUVS010000027 DASUVS010000000
VERSION     DASUVS010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6336
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663613"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VF182_01605"
     CDS             complement(<1..498)
                     /locus_tag="VF182_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6435794.1"
                     /translation="MAVDNLDLEVPTGICFGLLGPNGAGKTTTLRMIYGVTQPASGTI
                     LVFGMDISRNSRAVRARLGVTLQQNVLIEALSPKENLRVFGRYHLLQEPELSRRVEEL
                     IDFLELRSHADVPVKRLSGGFQRRLAIALSLINGPELLILDEPTTGLDPAVRLALWSR
                     IRELRA"
     gene            859..2058
                     /locus_tag="VF182_01610"
     CDS             859..2058
                     /locus_tag="VF182_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435795.1"
                     /translation="MSLDLMKEKGIGLDEQRFNWRDIAGMPTSKLDSDAFTRVRIILM
                     NGIESEALRFQHACARMNKELQIALARVRRIEQHQQTMVNWLLPADLSPLETTIGFEQ
                     VAIEVTASVAEHEPDPYLAQVFRFGLLEDFDHMYRFSALMDRVTGCDANNILQSYTDI
                     LPGRPTAVEHRAPEDDLREPYDRKKAAPISKLNAGTIMAGEHQTHDYYMTIGPMFADP
                     IARQLYAEIASIEEQHVTQYESIIDPEETWLEKWLLHEATEIYNYYSCLQYESHPKVK
                     EIWQRFLDYELGHFHYVLDLFKKFEKRDPAEIIPDSLPEPIEYRSHREFVRKTLSEEV
                     DLRALKTQFIDKDDEGADAASVIYREQMNSKGSPSETVAAGYQWKPGTELAEETPDIK
                     KLQTKVA"
     gene            2079..2957
                     /locus_tag="VF182_01615"
     CDS             2079..2957
                     /locus_tag="VF182_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX6435796.1"
                     /translation="METHEMDVQSNSKQESLGNRTGIMTNPELSIEMIEGANQTVPSS
                     QGDATELASVKAEYIEEAVPIGSSPVMVEVSEDGDSVGRFDGLAVLLDKLGERLAFER
                     QGTRLYEGFLQKCESITMDDASGPSIEELRHICEEELEHFHLLQNAITALGGDATVQT
                     PSADVAGVLSHGVMQVVSDPRTTIPQTLQAMLTAELVDNDGWQMLQDLAAELGQDDLE
                     EQCRRAYEEEQEHLEKVREWLTSMTMDEASGSTEELDASAEEEDTQPKRSIKSSTRSR
                     KSKNASASKSKKKKKR"
     gene            complement(2979..3614)
                     /locus_tag="VF182_01620"
     CDS             complement(2979..3614)
                     /locus_tag="VF182_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435797.1"
                     /translation="MNRVFLLSPANCNGVRAGWILRPEARSELAQRLRTPEGVSLAEV
                     FSFLSALYFRGKLAYARAFAQPPVPLSGVLLITPTAGLVPDGALVRMSTLRGFSRVPI
                     SIKNRRYRSALLRGVKQLAREIGPDCEVVLLGSIATGKYLDILNQALGDQLRVPAEFV
                     GLGDMSRGALLLRCVKEQRELNYISVAVASERVGRRALAPEAVQPSVETIQ"
     gene            complement(3611..5323)
                     /gene="polX"
                     /locus_tag="VF182_01625"
     CDS             complement(3611..5323)
                     /gene="polX"
                     /locus_tag="VF182_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX6435798.1"
                     /translation="MQDRFYIAGQLRQIGRLFSVMGENPFKIRAYERAARALENITGD
                     FDARIKERRLTEIEGIGKALAAVIEEIYHSGESSLLQQLTDELPPGAAELNQVPGLTL
                     KKIIALHETLGIKSVDDLKTACEKGLVRAVKGFGQKAEANVLAGIEKLQHRDDRILIS
                     HARADAEPLLQYLRGSSEIACCEVAGALRRCKETVRRISIVAASTDPKTVTERFLRYP
                     LFTRSDEAGTGHCVARLARGLEVEIHIVTPTGYAAALHYWTGSKDHYSALVDRARLQG
                     ITMSAEGLRGSTGKALKVETEANIYRRLGLEYIAPELRENQGEIEAAATGSLPKTVVV
                     EDIQGMIHCHTTYSDGQNTVEEMARAAEALGMHYITFTDHSPSASYAGGVKIDRLRAQ
                     WEEIDRVQERVRIKLLKGTESDILEDGLLDYPDYILEKFDVIIASIHTRHKMNADQMT
                     ARLLRALKLPVFKIWGHPLGRLIESRPPFDCHMDEVLDAVAASRAAIEVNGDPRRLDL
                     EPRWIRAARRRGIKFVVSTDAHSIRNLENLPYGVAMARRGWLSRDDILNSLPVEKFME
                     AVHP"
     gene            complement(5397..5918)
                     /locus_tag="VF182_01630"
     CDS             complement(5397..5918)
                     /locus_tag="VF182_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435799.1"
                     /translation="MKIIRQHPLLLGVIFALGVLLGVGIMSWRPDDQSARMGYRDLSN
                     DELSEKGVTMARALRALAREMMATDEKLRSRCDRENELAKTEQAKNQLRQSCNDESLK
                     ATQNFLAQYEDKYRAEVLILREEMLRRLPELRQKAAPPVLASHPTNALGLGEVASTLE
                     VLAKLLPKMPSKA"
BASE COUNT         1541 a         1765 c         1696 g         1334 t
ORIGIN      
        1 tgcgcgcagc tcccggatgc gcgaccacag cgcaaggcga actgccgggt cgagacccgt
       61 cgtcggctca tcgagaatca gcagttcagg gccgttgatc agagacaaag cgatggcaag
      121 tcgacgctga aagcctccgg acagccgttt caccggaaca tcagcatgag agcgaagctc
      181 gagaaaatcg atcagttctt caacgcgccg cgatagctcc ggctcttgca acagatgata
      241 ccggccgaag acccggaggt tttcttttgg ggacaacgcc tcgatcaaaa cgttctgttg
      301 taacgtaacc ccaagccggg cgcgcacagc tcgggaattc cggctgatat ccatgccgaa
      361 aaccaggatg gtaccgctcg cgggttgggt gacgccgtaa atcatccgca acgtagtggt
      421 tttcccagcc ccattgggcc ccagcaaacc gaagcaaatc ccagtcggca cttccaggtc
      481 gagattgtcg actgccacac ggtcgccgaa ctgctttgaa acggcgtgca tctgtataac
      541 gggcgcctgt gaagggatca tgcagaacca tcgctgataa atatctctta aatctgacct
      601 atcttcgctc gaaccacaag gcggcgacta cgcaggacgg cccagatatc aatcaactac
      661 ggaacagtct taagagaaat gtcgcaacgc ggagactaag gtatcggccc aacacgtttt
      721 ttatactggc acctcgcttg cacaaattgc acgatagctt ctcgttcgca aatttctgca
      781 caattgaatg ttctgtaaac gacgtcccta aagcggctga ctaactacgg caacggttcc
      841 acatgaaagg agagtggtat gagtttagac ctgatgaagg aaaaaggcat tggcttggat
      901 gaacaaaggt tcaattggcg cgatatcgcc gggatgccga caagcaaact cgacagcgat
      961 gcttttaccc gggtgcggat tattctgatg aacggtatcg aatccgaggc gctgcggttt
     1021 caacacgcct gcgcccgcat gaataaagaa ttgcagattg ccttggcgcg agtgcggcgc
     1081 atcgaacaac atcaacaaac catggtcaac tggttgttac ccgccgatct ttcgccgttg
     1141 gaaaccacca tcggcttcga acaggtggct atcgaagtga ctgcgagcgt ggcggaacac
     1201 gaacccgacc cttacctggc gcaggttttt cgcttcgggt tgcttgagga tttcgatcat
     1261 atgtatcgct tctccgcctt gatggaccga gttacgggtt gcgatgccaa taatattctg
     1321 cagagttata cggacattct gccggggcga cccactgccg tggaacaccg cgcgccggaa
     1381 gacgatttac gcgaacctta cgatcgcaag aaggcggcgc cgatcagcaa gctcaacgcc
     1441 gggacgatta tggccggcga gcatcaaaca catgactatt acatgaccat tgggccgatg
     1501 tttgccgacc ccatcgccag gcaattgtat gccgaaatcg cttccatcga agaacaacac
     1561 gtcacccagt atgaatcgat tatcgaccct gaggagacct ggctggagaa atggctgctg
     1621 cacgaagcca ccgagattta taattactac agctgtctac aatatgagag ccatccaaaa
     1681 gtaaaggaaa tctggcaacg gtttctcgat tacgagttag gacacttcca ttatgttctc
     1741 gatttgttca aaaaatttga aaagcgtgac ccagccgaga tcatccccga ttcattgccc
     1801 gaacccatcg aataccgcag ccatcgcgaa ttcgtacgca aaactctgag cgaggaagtc
     1861 gatttacgcg cgctgaagac tcaattcatc gacaaagatg acgagggcgc cgatgcggcg
     1921 tcagtgatct accgcgagca gatgaattca aaaggctcgc cgtccgaaac cgtggccgcc
     1981 ggctaccaat ggaagccagg taccgagttg gccgaggaaa ctcccgatat caaaaagctg
     2041 caaaccaaag ttgcataagc aaaccaggag accatcatat ggaaacgcat gaaatggacg
     2101 ttcagtctaa ttcgaaacag gaaagtctcg gtaatcgtac cggcatcatg accaatccag
     2161 aactgagtat cgaaatgatc gagggagcca accagacggt gccgagctca caaggcgatg
     2221 ctacggagct cgcatcggtt aaggccgagt atatagagga ggctgtgccg attggctcat
     2281 cgccggtaat ggtggaagtc tctgaagacg gcgacagcgt cggacgcttt gatgggttag
     2341 ccgttctgct cgacaaatta ggcgagcggc tggcgttcga gagacaaggc actcgcctct
     2401 acgaggggtt cttacaaaaa tgtgagtcga tcactatgga tgatgcctcg gggccttcta
     2461 tcgaagaact gcgccacata tgcgaggagg aactggagca ttttcacctg cttcaaaacg
     2521 ctatcaccgc tttaggcggg gatgcgacgg tccagacgcc gtcagcggat gtcgctgggg
     2581 tattgagcca cggcgtcatg caggtagtca gtgatccgcg cacgacgatt ccgcagacat
     2641 tgcaggcgat gctcacggca gagctggtcg ataacgacgg ctggcagatg ctccaagatc
     2701 tcgccgcaga gcttggtcaa gacgatctcg aagagcagtg ccgcagagcg tacgaagagg
     2761 aacaggagca cctcgagaaa gttcgcgagt ggctgacaag catgacgatg gacgaagctt
     2821 caggatcgac ggaagagctc gatgcttcgg cagaggaaga agatacccag ccgaagcgat
     2881 cgatcaaatc gtcgacacgg tcgcgcaaaa gcaagaacgc gagtgcatct aaaagcaaga
     2941 agaagaaaaa aagatagcac tattcagagc gccggtactc actgtatggt ttcaactgac
     3001 ggttgcactg cttccggcgc gagggcgcgt cgcccgacgc gctcggaagc aactgcgacc
     3061 gagatatagt ttaactcccg ctgttcttta acacaccgaa gcaggagcgc gccgcggctc
     3121 atatcaccga gaccaacaaa ctcggcagga accctcaatt gatcgccgag cgcttgattg
     3181 aggatgtcca ggtattttcc ggtcgcgatg ctgccgagca gaaccacttc acagtccgga
     3241 ccaatttcac gggcaagttg cttgacccct cgcaggagag cgctccggta gcggcgattc
     3301 ttgatactga ttggaacacg gctaaagccg cgcaatgtcg acatccgcac caacgctccg
     3361 tcaggcacca aacccgcagt cggagtaatc aatagaactc ccgataacgg gaccggcggc
     3421 tgggcaaacg cccgcgcata ggcaagcttg ccgcggaaat aaagcgcgct aagaaaactg
     3481 aagacttcgg cgagagacac tccttccgga gtgcgcaagc gctgggcgag ctcggaacgg
     3541 gcttcgggcc ggagaatcca gccggcccgc actccgttgc agttggcagg cgaaagtaaa
     3601 aaaactcgat tcacgggtgg accgcttcca taaacttttc gaccggcaag ctatttagaa
     3661 tatcatcgcg cgacaaccag ccgcggcgcg ccatggccac tccgtacggt aggttctcca
     3721 gatttctaat cgaatgcgca tccgtcgaca cgacgaattt gattccacgc cgccgtgcgg
     3781 cgcgaatcca gcgcggctcg agatcgagac gacgcgggtc accgttgact tctatcgccg
     3841 ctcgagaagc ggcaaccgca tcgagcactt catccatgtg gcaatcgaac ggcgggcgcg
     3901 actcaatcag ccggccaagc ggatgtcccc agattttaaa taccggcagt ttcagcgcgc
     3961 gcaacaaacg tgcggtcatc tgatcggcgt tcatcttgtg gcgtgtgtgg atgctggcga
     4021 tgatgacatc aaacttttcc aaaatgtagt ccggataatc gagcaagcca tcttcgagaa
     4081 tgtccgactc ggttcctttt aacaacttga tccgtacccg ctcttgaacc cgatcgattt
     4141 cctcccactg tgcccgcaag cgatcgatct ttactccgcc ggcataggac gcacttggag
     4201 aatggtccgt gaaagtgatg taatgcatac ccagggcctc ggcggctcgc gccatttctt
     4261 caaccgtgtt ttgcccatcg gaatacgtcg tatggcagtg aatcatgcct tgaatgtcct
     4321 cgacgacaac cgttttgggc aagctacccg tggcggcagc ctcgatctcg ccttgattct
     4381 cgcgcagctc cggcgctatg tattccagtc ccaagcgccg gtaaatattg gcttcggttt
     4441 caaccttcaa cgctttacct gtggaaccgc gcaaaccttc ggcactcatg gtaatacctt
     4501 gcaaccgcgc gcgatctacc agagcggaat agtgatcctt cgatccggtc caataatgca
     4561 aagcggcggc gtaccctgtc ggcgtcacga tgtggatctc gacctccagt cctctggcga
     4621 gacgcgcgac gcaatgccct gtgccggctt catcggatcg ggtgaataat gggtagcgca
     4681 gaaaacgttc ggtgacggtt ttcggatctg tgctggcggc aactatgctg atccgccgca
     4741 cagtttcttt gcagcgccgc agcgcgccgg caacctcaca gcaagcaatt tccgaggagc
     4801 ctcgcaaata ttgaagcagc ggttcggcat ccgcccgcgc atgcgagatg agaattcggt
     4861 catcgcgatg ctgcaacttc tcgattcccg caagaacgtt cgcttcagct ttttgaccga
     4921 aaccttttac agcgcgaaca agccccttct cacaggctgt tttcagatca tcaacgctct
     4981 tgatccccaa agtttcgtga agcgcgatga tctttttaag ggtgagcccc ggaacttgat
     5041 tgagctcggc ggcgcccgga ggcagctcat cggttaactg ctgcaataga gagctctcgc
     5101 cggagtggta gatttcctcg ataacggctg ccaaagcttt gccgatcccc tcgatctcgg
     5161 ttaagcgccg ctctttgatc cgcgcatcga agtcgcccgt gatgttttcc aacgctcggg
     5221 ccgcccgctc ataggctcgt attttaaatg ggttctcgcc catgacgctg aacaaccgac
     5281 cgatctgcct caattgtccg gctatataaa atctatcctg cattggaggg actgctccgg
     5341 atttccatac agattaaccg ccgagctctg acggggcaat agggcttcga ctaggattaa
     5401 gctttagacg gcattttggg tagcagctta gcaagtacct ccagggtgga ggcgacttcc
     5461 cctaaaccca aagcattggt agggtgactc gctagaacgg gtggcgcggc tttttgtctg
     5521 agttccggta gtctgcgcag catctcttcg cggagtatca gcacctcggc tctatatttg
     5581 tcctcgtatt gggcaagaaa gttttgcgtg gctttgagcg attcatcatt gcacgattgc
     5641 cggagttgat ttttggcttg ttcagtttta gccaactcgt tttctctatc gcagcggctt
     5701 cgaagctttt catccgtagc catcatttct ctggcgagcg cgcgtaacgc acgcgccatt
     5761 gtgactcctt tttcggacag ctcatcgttc gacagatctc gatagcccat ccgggccgac
     5821 tgatcatccg gccgccagct catgataccc acccctagaa gcacgccaag ggcgaaaatt
     5881 accccgagca gcaacggatg ctgtcggatg attttcatct gacttgtgaa actgataacg
     5941 acactccgga cggcacatcc cctttttacc catcgttggt agggagtcaa tgcatgccac
     6001 acgtggtgca gccggaatat ccaggatact cagtcagctc gagagacgtt gaccggcaac
     6061 ttcaaagccc ggcaagctcc caccggaact ctaattcacc gtctctcttt cattgggcag
     6121 tcgtaatgca aacccaccgc aattggaggc gcaacgtcgc gtttctgcat ttcaaagtgg
     6181 aagcatcatc taacctagga aagcccagcg gtggagacga acctctggct ccacggcgcc
     6241 ggagaccggt cggtatcagg cggctcaagt tatcattaaa tacccaacac ccgtgcgtgc
     6301 tgctgcacca acggaaaagg cgggcgatgg taccat
//
LOCUS       DASUVS010000028        18166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_663801, whole genome shotgun sequence.
ACCESSION   DASUVS010000028 DASUVS010000000
VERSION     DASUVS010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 18166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18166
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663801"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(345..1430)
                     /locus_tag="VF182_01635"
     CDS             complement(345..1430)
                     /locus_tag="VF182_01635"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6435800.1"
                     /translation="MSDCRAAVITAHNQPLTIERVPIPDLEPGALLVKIAASTLCGTD
                     VHRWHGPLGDGDTLPIITGHEPCGYIEEINGERTDIFGQPVKRGDRIVWSYVACGSCY
                     YCSVALQPCICPGRASWGHNRSDQYPYLLGSCAEYMYVPPACLIIKVPDEVSSASAAA
                     SACAYRTVMHGFDRLGAIKSNETVVIQGSGPLGNFATAVAKDHGAKKVLVIGAPAARL
                     EVTRRMGADGVLNLEEVTDAKDRRQWVHDHTDGRGADVVIQVANNMAVPEGLTLLRGG
                     GRYLNIGAGGKADIAVERLPQEMTLVTVRSAEPRHWLQAIDFLASRRDRFPFEAMITA
                     HYDLEHVNEALQAMASYQVVKAVIDLN"
     gene            complement(1581..2666)
                     /locus_tag="VF182_01640"
     CDS             complement(1581..2666)
                     /locus_tag="VF182_01640"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6435801.1"
                     /translation="MSSSLATRIIDGDGHIIEDHAGIIAHMETPYREIANRKGIVFPP
                     LDHLHTGRAVETPPQRDQRPNVGPKGWLDFLDDVGIDWTVLYPTTALAYGKIVSLDYA
                     VAAARAYNDWLCETYIKYNPRFKGMAIIPMQDPEEAAKELRRAVTELGMLGAMMPSNG
                     LPQPLGAKAYWPIYAEADRLGCCLAIHGGAHDRFGMDHMNMYVPVHALGHPWGLTFNC
                     ANIVYNGIFDRFPRVRIAFLEGGVAWLLLLLERLHASHETHFQYIPPSEFGIREEDDP
                     SKYIKKQIQEGRFFLGIETEELTLPFAIKIVGNSPFLYSSDFPHEVTNESCKHDLGEL
                     MESEEITAADKAAMLYKNAENFYRLPL"
     gene            complement(2846..3937)
                     /locus_tag="VF182_01645"
     CDS             complement(2846..3937)
                     /locus_tag="VF182_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6435802.1"
                     /translation="MAVSIKPLIVVEDDSFLRLIQLVLDPTAPRELFEAFAHFCAHDV
                     PDFRGWCEQASARVRHLYPADVRLVRDCGELLANIPGANALVVEALPAGAHEIAAAGG
                     TLRLVQRYGTLTRNIDEAACRRAGIKIKTLRRRANISTAEQALALMLALARQLNQNAN
                     LISIEQIEAAGYTPTQYDRARTPNGNWARVTGLKTLFARQLGIIGLGEIGRELALRAA
                     ALGMRITYHQRHRLTSAEEQHYQASYSSRTQLLETSDCVSLHLPRGAETRGIIGRAEL
                     ENIKPGALLINVSQPSLIDRAALLDALESGRLGGFAMDNFYEEPGDPDDPLIKFRNVI
                     ITPHLAGSPRSNSLDDIAELLDNLANGLS"
     gene            4116..4367
                     /locus_tag="VF182_01650"
     CDS             4116..4367
                     /locus_tag="VF182_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435803.1"
                     /translation="MAEETAEQKLARLEAENQALRQQVERKPGQLRLKVSEKGGLSLY
                     GLGRFPVTLYKEQWVKLLDHAEEIRSFLKDNDKQLKAKE"
     gene            4462..5535
                     /locus_tag="VF182_01655"
     CDS             4462..5535
                     /locus_tag="VF182_01655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX6435804.1"
                     /translation="MSSSSMPINLFDFEAIAKERLPKGEYDYIAGGATDEISVKRNRR
                     AFESWALRPRVLRDVSALDLSTTVLGTKVNLPVLIAPCGGHKKAHPEGEFATYRAAAA
                     RGTIMAVSANSNSSFEELAKAASGHLWLQLYPFRDRNLTKSWLDRAKEAGYDAICVTL
                     DSQWPPKRERNIRNNYRRTRGINYPAQPLAPATAPRPAGAEGGSDPAATWKDFEWIKS
                     QTSLPVVAKGIMTGEDVELCAEIGIDGVIVSNHGGRHLDNTLATVEVVAETVAAAKGK
                     LEIFLDGGIRRGADVVKALALGAKAVFIGRPLFWGLAVNGEQGVVRVLEILREEMEIT
                     MAKCGRPTIASIDSSVMVKVPRL"
     gene            complement(5802..6722)
                     /locus_tag="VF182_01660"
     CDS             complement(5802..6722)
                     /locus_tag="VF182_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6435805.1"
                     /translation="MNRALLIGTSGYSYPGPPPAGWAGAFYPEPKPKRFDELHYYSQI
                     FNTVEINSTFYRPPSITTTTAWAKKTSSDFTFALKLWQKFTHPVKIGRKITDEKWQPP
                     TQDDIDQFRAGIEPIAEAGKLGALLLQYPTSFHCTPENMERVERTLQVFYDYPKVVEL
                     RHNSWDEKSEEIKALLEANRAGSVLIDEPKFSSSIRQGLEIVGDFFYFRAHGRNAKAW
                     WRPKESWERYDYLYSRREIKKIAERIESATSAPGIKKGFAFFNNHARANAAANAIMLY
                     QELGLRPKAMPGEAMIAKFPELANVEPSST"
     gene            complement(6956..10408)
                     /locus_tag="VF182_01665"
     CDS             complement(6956..10408)
                     /locus_tag="VF182_01665"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX6435806.1"
                     /translation="MRIRSHLLFLVLGTLLPILAFSAAMTAVFWREQRQVLEQRFLDR
                     VRGMTVALDREIDGNIRSLQMLAESPHLQFNDLLSFHEQIARALSTQSTWANIILSDP
                     ASGKQVINLRYPFGTRLSETTLDKTDLAAVVKSGRPFVPPILKGRAGARYTTAIVVPA
                     KTGRGRQYTLVAVIDPASWLRFLSNYPVATDATLTLLDQNGIIIARTLNSERWVGQPP
                     SKALSAEARKAPEGSSITQGLEGQWFYTAHSRSKLAGWTLASGVPKEGVEAALRQSVL
                     LMVAGVAATAILAITLAVVLGRRIARPISELAHTAQALASGERVSAHWPTEVAEVDDV
                     RAAFSSAADRLRAKDSALRDSEERFRSMADTAPVLLWLSDVDQQCTYINKPWLDFTGR
                     RFEQELGEGWTESIYPEDRRRCVETYRASFQRRERFEIEYRLRRADGEYRWVLTRGAP
                     RVTDTGELIGYVGSCVDITERKRVEESLRESEARLRLAMQTGKVGVWDWDIAANRVVW
                     TESLYAIHGVRPDEFVTTIEAFATLLHPEDRDLVLHSLQRALEHDAPYELEFRAIRPD
                     GQIIWLFTNAMVLRNAGEPFRMLGATMDITQRKLAEETVLRLLRISERLNSTLDVDTL
                     LDVLVDEAIRIVDGESGVSGLYTPDGMVCRKYLQKGKAVPFDYCWRPSEGLPGHIISE
                     KLPYLTNEADGEPRTAHEVYARFGVRSALSIPILNFQRELIGFFEIHNKTSAGGFTDL
                     DKRTLVAISEAAATAIQNSLAYRRVQEAEESLRDVDRRKDEYLAMLGHELRNPLGVIS
                     ISAQLLRRQEPSSPELAELRDIVDRQVEHMTRLLDDLLDVSRISRGQMKLNQTLCDFS
                     SLVRQTMQDYRSKLETNNLHLEIDIPDRPMWVMGDRTRLAQTISNILHNADKFTGAGG
                     IITVALQEEAAAKQVILTVHDTGIGMEPELLARVFEPFTQGDHGIDRRPGGLGLGLPL
                     VKGLIELHGGQVRASSAGIGRGFQITIRLPLRDQAAPTARRRSSASVHPTARRILVIE
                     DNRSAARTMNVYLTRQGHTVEAAHTGSEGLQAARRFQPDVILCDIGLPEFDGYKVARQ
                     LRQDPELNRVYLIGISGYGQEHDKERAWQAGFNAYLVKPVDMSELESLLVKFQTDASI
                     CGMM"
     gene            10643..12031
                     /locus_tag="VF182_01670"
     CDS             10643..12031
                     /locus_tag="VF182_01670"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6435807.1"
                     /translation="MSEVVNNLAHIPAGNFSENTSSFFLIEMTQKRVDVRESLRSSFK
                     DGIFAAFMAGVTEYYATPLALFLGATVQQVGLISAVPQLLASVSQFLAVRIIHWIGGR
                     LKLLVRLVLCQALLIFCIAILPWLETARRVELLIVLLILAAVCGGLAGPAWGSLITDY
                     IPSSKRGRYFGWRNRTVGAVTLVSIILSGLVLYWFGEISYRAGFCLLFGSAALARFAS
                     GYFISRMDEPPHRSDPATDFTFLMFIARFRESNFLRFVVFSACLTFATYLSAPFFAVF
                     MLRDLQFSYLTYMGLQVCSSFASLVALPLWGRHADLVGNVRVLRLSSFLAALIPAFWL
                     ISHDPAYLMLVQIWGGFAWSGVTLSAANFIYDAVTPQKRVRCIAYFNVINGAALFLGS
                     ALGGLLGSQLPPLLGYGLLSLFAVSCFCRLGFYLLLARSFREVRPTQKASTRELLFSV
                     VGIRPLIGRSQE"
     gene            complement(12086..13147)
                     /locus_tag="VF182_01675"
     CDS             complement(12086..13147)
                     /locus_tag="VF182_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6435808.1"
                     /translation="MTLILSNDDIQKAMTVKESLAVMEESYREQAASRAVNRPTCHSY
                     LPHSLPNSTYSFKSVDGGIGKYGVLALRVTSDVVQEQTINNTVRLEKIPLAGKGLFLG
                     LVQLFSAETGELLAIMPDGFVQQTRVGVTSALAMKFMARKNSKTLGLIGSGGQARAHY
                     RYMTAVLPIEKVKVFSPNREHCRNFTEQMSKETGVAGQAVNSPEEAVKGCDIICSATN
                     SSRPVVNIEWLEPGMHYNSIREFETDIGALEKCDVVAIHTQFGGIYHYQPPGIVEDMP
                     GVRREKPRDWSRYPEICDIIAGKVIGRANDRQITFFLNNVGTGVQFAAMGYCAFKAAK
                     EHGLGHEIPSEWFLQDIKP"
     gene            13817..14902
                     /locus_tag="VF182_01680"
     CDS             13817..14902
                     /locus_tag="VF182_01680"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6435809.1"
                     /translation="MTLILNNDEIESVFTLEQCFDVLEPALRDLGIGSAVSMPRQDLL
                     VPGPLDGSFHGLKTSCASLPRSGVTTMRLTSDILTWPVVGGRQRRVKVPLAVGSKYVG
                     LVFVFSTATGELLAILPDGFIQAIRVGVTNALSAKYMARPDSYVMAVYGSGWQARPAV
                     LAMCKVLPISEVRVFSPTKSNCERFVAEMQQSIDARIIAVSSPEEAAQNADVVSLATN
                     ALDPFFPASWLGPGMHVTTVRPSEMMLEALLRCDRIAVSTREAAQLFTLPGQESAVPE
                     FGKGDYGRAELEKTAADWRDKAELSEIIAGKVKGRDKDSDVTCMLNHLGLGLQFSAAA
                     ARVYELAREKGLGRELPTEWFSQNEHS"
     gene            complement(15210..15929)
                     /locus_tag="VF182_01685"
     CDS             complement(15210..15929)
                     /locus_tag="VF182_01685"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435810.1"
                     /translation="MYGWRAKIGRISPSPETVGAEEWRRSMPEGVCLVETRTLLYDVT
                     VDGLSETAEQVERAALELASAEVDVILQAGTAIAFFRGFGHDRELSRRIHAATGIQAT
                     TSLTAVVEALRALEIQRPAIATPYLTDIDARLVEVLEKSGFEVAAIRGMGLKKSIDMG
                     KVTPEETYRLAREVVRSAPKADGILISCGNLRTFEAIEALEIDTGLPVVTSNQAGLWQ
                     ALRMTGIRDRLTSLGRLLRES"
     gene            complement(15931..16920)
                     /locus_tag="VF182_01690"
     CDS             complement(15931..16920)
                     /locus_tag="VF182_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX6435811.1"
                     /translation="MRAVRIHELGGPDVLRYEEAPAPQPKAGEVLVRVAAAGVNYLDI
                     YYRSGFHWGGHHGRALPYTPGAEAAGTVVDLGANVTEIKIGDRVAYGISNGYGSYAEL
                     HAVPAWHLYKIPQSIAFEDAAAIMLQGMTAHYLTHSTYRVKPKDTVLIHAAAGGTGLL
                     LVQIAKMRGARVFGTVSSDEKARLAQAAGADRTINYTEQDFAAEIRELTDGQGVNAVY
                     DSVGKATFEESLDCLAPLGTLVIFGQASGPVPPFDTSILNAKGSLTLARPSLTHNVAS
                     HADVLWRAGDLFNWLAEGKLAIRIGGIYPLSQAADAHRLLESRTSAGKILLLP"
     gene            complement(17083..17820)
                     /locus_tag="VF182_01695"
     CDS             complement(17083..17820)
                     /locus_tag="VF182_01695"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX6435812.1"
                     /translation="MTTLLAPKGSTSMRIVDLSMTVQECDSAPFAPDENYFKLRPIIK
                     WEDKGFVSNVVQMTVHVGTHIDSPHHFFRDKPSIEQLPLEPMVGQAIILDLTAKGVAR
                     AVITPEDLSEAEKKLQQQGIEIPQGGTLFLRTDWPKGHDTTDPQWWNDSPYLNKEAAQ
                     WIVAKKPAVVGFDFAQEEKGNDYQSADQILESAMRVHRTILPRVIFQIENLINLDQIG
                     SKAQVIALPVKWKTESAPARVIALLDN"
     gene            complement(17844..>18166)
                     /locus_tag="VF182_01700"
     CDS             complement(17844..>18166)
                     /locus_tag="VF182_01700"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435813.1"
                     /translation="DQIITAVMKRVCLVQNPRLRSVGNLGCQAEVEIQLNSGKTYRKL
                     AAVGKGHPKKPLTREEIQEKFYECAGERIPKKRAKRFVEKLWSIERVDALGPWLRSLR
                     FPRG"
BASE COUNT         3917 a         4938 c         5004 g         4307 t
ORIGIN      
        1 atccgaatag ctagttgtcg aggccgggtc gacggctacc gattttagtc gtcggcccgc
       61 ttaattctct gaagtattga ttccctataa gcgggactct tgtgtgccgt ctttatcaac
      121 taacgacccc gcctaattct aatctcgacc ttattcgaga atgtagattt tgaaacggct
      181 gctcgtggca gccgatgcga acaacatccg atggtgcgca tctacataag tttaacggcg
      241 cggccaattc gctattaaga tcacttggag ccagaagtca gctgaaaaaa aatcagcggc
      301 aaaaggtaaa agcgctactc tgtggcagag gtgaagctgt catctcagtt gagatcgatt
      361 acggctttta caacctggta gctcgccatc gcttgcagcg cctcgtttac atgttcgaga
      421 tcgtaatgtg cggtaatcat cgcctcgaac ggaaaccgat ctcgccgcga cgccaaaaaa
      481 tctatggctt ggagccagtg gcgcggttcg gcggatctga cggttactag cgtcatctct
      541 tgcggcaggc gctcaaccgc aatatcggct ttgccgccgg cgccgatgtt caagtagcgc
      601 ccgccgccgc gaagcagggt caatccttcc ggcacggcca tattattggc cacctgaata
      661 actacgtcag cgccacggcc gtcggtgtga tcgtgcaccc attggcgccg gtctttggcg
      721 tcggtgactt cctctaaatt cagcactcca tccgcgccca tgcgccgggt aacttccaat
      781 cgagccgcgg gcgcgcctat gaccaagact tttttcgcgc catgatcttt ggcgactgcg
      841 gtcgcaaagt tacctaatgg cccgctgcct tgaatcacca cggtctcgtt gctcttgatt
      901 gcgcccagcc ggtcaaaacc atgcatcacg gtccggtagg cgcaggcgga agcggccgcg
      961 gaagcggatg agacctcgtc gggcactttg ataatcagac acgcaggcgg cacatacatg
     1021 tattcggcgc agctacccag gagataagga tactgatcac tgcggttatg cccccatgag
     1081 gcgcgccctg gacagatgca aggttgcaaa gcgacgctgc agtagtagca gcttccgcaa
     1141 gcgacataac tccagacgat gcgatcgccg cgcttgaccg gttggccgaa aatatcggtg
     1201 cgttctccgt tgatttcttc gatgtaaccg cagggttcat ggccggtgat aatcggcagg
     1261 gtgtccccat cgcctaatgg tccatgccag cggtgcacgt cggtgccgca cagtgtagag
     1321 gcggcgattt tgaccagcag cgctccgggc tcaagatccg gaatcggcac ccgctcaatc
     1381 gtcaacggtt gattatgggc agtgataacg gcggcgcgac aatcgctcat gaacaataaa
     1441 cctccagctt agtggcaata cgtgatcgat tacgaaaaaa tcccgctgtg gcataccgat
     1501 agaaccgccc gtattcagcg caaactcaga agagcttcgc cgtaattgga ccaacttcag
     1561 gcactcgctg cgtctcagtt ctacagcggc agtctgtaga agttttcggc gttcttgtac
     1621 agcattgcgg ctttatctgc cgcggtgatt tcttcgctct ccatcagctc gccaagatca
     1681 tgcttgcagc tttcgttggt tacctcatgg ggaaaatcag aggagtagag aaatggcgag
     1741 ttgccgacaa tcttgatggc aaaaggaaga gtcagttcct cggtttcgat gcctaagaaa
     1801 aagcgccctt cctggatttg ttttttgatg tatttgctgg gatcgtcttc ttctcgtatg
     1861 ccgaattcgg atggcgggat atactgaaaa tgggtttcat gcgatgcatg aagccgttcc
     1921 aacaggagca gcagccaagc caccccccct tcgagaaatg cgatccgcac tcttgggaat
     1981 cggtcgaaga taccgttgta aacgatgttg gcgcagttga aggtcaatcc ccaagggtga
     2041 cctaatgcat gaaccggcac atacatgttc atgtgatcca tgccgaaacg atcgtgcgcg
     2101 ccgccatgga tggcaagaca gcagccaagt cggtcggcct cggcataaat cggccagtac
     2161 gccttggcgc ctaacggctg cggcaaacca ttagagggca tcatggcgcc caacattccc
     2221 agctcggtca ccgcccggcg cagctctttg gcggcttctt ccggatcttg cattggaatg
     2281 atcgccatcc ccttaaagcg cggattgtat ttgatgtagg tttcgcaaag ccaatcgttg
     2341 taagcgcgcg ccgcggcaac cgcatagtcg agactgacga tcttgccgta agccagagct
     2401 gtggtcgggt aaagcaccgt ccagtcgatc ccgacatcgt cgaggaagtc gagccaaccc
     2461 ttgggtccaa cgttcgggcg ctgatcgcgc tgcggcggtg tttccaccgc ccggccggta
     2521 tgaagatgat ccagtggcgg aaatacgatg cccttgcggt ttgcgatttc ccggtaggga
     2581 gtttccatat gggcgataat ccccgcatgg tcttcgataa tatgaccgtc gccgtcaata
     2641 attctggttg ccagtgagct gctcatgcgt cctcctgtaa atcaccaaat ataacaatca
     2701 gcgctgtcga aaagcgcaac gcctaaaaac gacaggtttt tcgaacaacg gagtccaatc
     2761 ttttgagcct tatatataaa ccggagttaa gttgcgtatc aagttcgtgc ttttctaatt
     2821 cgaagttccg ccatcaaagc actggctatg acaagccgtt cgctagattg tccagcaact
     2881 ctgctatgtc gtcgagtgaa ttcgacctgg gtgaaccggc caggtgagga gtgattatga
     2941 catttcgaaa ttttatcagc ggatcatcgg gatctcccgg ttcttcataa aaattatcca
     3001 ttgcgaaacc gccgagccgc ccagactcta gagcatccaa cagagcggcg cggtcgatga
     3061 gtgaaggctg tgacacattg atgagcaatg cgcccggttt gatgttttca agttcggctc
     3121 ggccgatgat gccgcgagtt tccgctccac ggggtagatg caggctcacg cagtccgatg
     3181 tttctagaag ttgcgtccgg gaggaatagc ttgcctgata gtgctgttct tctgccgacg
     3241 tgagacgatg gcgctggtga taggtgatgc gcatacctag cgccgctgcc cgtagcgcaa
     3301 gctcccggcc aatttcaccc aagccgatga tgcctaattg ccgagcaaaa agggttttga
     3361 gtccggtgac tcgcgcccag ttcccgttgg gcgttcgcgc tcggtcgtac tgcgtcggtg
     3421 tatagccggc tgcctcgatc tgctcgatgc tgatcaaatt ggcgttctga ttgagctggc
     3481 gcgccaaagc aagcatcagt gcgagcgctt gctcggccgt ggagatattg gcgcggcgcc
     3541 tgagcgtttt tattttaata ccagcgcgtc ggcacgcggc ttcatcgatg tttcgagtaa
     3601 gggtgccata tctctgcacg agacgcaggg tgccgcccgc tgcggcaatc tcgtgcgcgc
     3661 cggccggcaa cgcttcgacc accagcgcgt ttgctccagg aatatttgcc agcaattcgc
     3721 cgcaatcgcg caccagccgg acatcggccg ggtacaaatg acgcacacgg gcgctggctt
     3781 gctcgcacca accgcgaaaa tccggcacat catgggcgca gaaatgagca aaggcctcga
     3841 atagctcgcg gggcgcagtt gggtcgagaa cgagctggat cagccttaga aacgaatcgt
     3901 cctcaacgac gattaacggc ttgattgaaa cggccatcgc ttggtcctta tgcgtatccg
     3961 aatgttaacc aacctcgcgg cgctcgtgaa cacaggaaag agaatgtcga tctcgcgttc
     4021 tttgccgctt tgcgataaga aattgttctt aagacaagag caacgattct gacattgcag
     4081 gacaaaaaat cccggcaaaa actgttggag ggattatggc agaagaaaca gctgagcaga
     4141 agctggcgcg gcttgaggcg gagaatcagg cgctgcgtca acaggtggag cgaaagcccg
     4201 gtcaattgcg cctaaaggtc agcgaaaaag gcggtctgtc gctttacgga ctcggcaggt
     4261 tccctgtgac tctatacaag gagcagtggg tgaagcttct tgatcatgcc gaggagatcc
     4321 gatcctttct caaggacaac gacaaacaac taaaagccaa agaatagcgg gtttgaacag
     4381 aatagattaa tcctacagac gcaagcctcg ttaccactgc aaatcagttc cgcgaacggc
     4441 agagtttact tggagggtga gatgagcagc agttcgatgc cgatcaacct ttttgatttt
     4501 gaagcgattg ccaaggagcg cctgccgaaa ggggaatacg actatattgc cggcggcgcc
     4561 accgacgaga tcagcgttaa acgaaatcgc cgcgctttcg agtcttgggc actgcgcccg
     4621 cgggtgttgc gcgatgtgag cgcgcttgac ctttcgacga ccgtgctcgg aaccaaggtg
     4681 aacctgccgg tgctgatcgc gccgtgcggc gggcataaaa aggctcatcc ggagggcgag
     4741 ttcgcgacct accgcgccgc cgcggcccgg ggcacgatta tggcggttag cgccaattca
     4801 aactcatcct tcgaagaatt ggccaaagcc gcctccgggc atctgtggct gcagctctat
     4861 ccatttcgtg atcggaacct gactaaaagc tggctcgatc gcgctaaaga agcgggctat
     4921 gacgcaattt gtgtcactct ggattctcag tggccgccca agcgagagcg aaatattcgc
     4981 aacaattatc gaagaactcg aggcattaat tatccggcac aaccgctagc gccggccact
     5041 gcgccgcgac ctgccggcgc cgaaggagga tcggatccgg ccgccacttg gaaagacttc
     5101 gaatggatca aatcgcaaac cagcctgccg gtcgtcgcca aaggcatcat gacgggcgag
     5161 gacgtcgagt tgtgcgcgga aataggcatc gatggagtga tcgtatcaaa tcacggcggg
     5221 cgtcatctcg acaatactct ggcaaccgtg gaagtcgttg ctgaaacagt tgccgccgca
     5281 aaaggaaaat tggaaatttt tctcgacggc ggcatcaggc gcggcgccga tgtggtgaaa
     5341 gccctggctt tgggagccaa ggcggtcttc attggccgtc cgcttttttg gggtctggct
     5401 gtcaatggcg agcagggagt agtgcgagta ttggaaatac ttcgggaaga gatggaaatc
     5461 actatggcta aatgcggccg cccgacaatt gcgagcattg attcgagcgt gatggtcaag
     5521 gtgccgcggc tgtaacaccg tatatcgagc gccgctgggt gagtagaaag aacggagatg
     5581 ccgtcaaacg aattgaatct ttcgactatt aataatacct gttaacctct ctgctttcgc
     5641 ttcgtctaat cttttagaac cgatgctcca gcgcacgaat ctatcgcaag gtgagacgca
     5701 gcatgtttgg attcttattg ttggccgtcg tcttgatcta tgtagcgatc tcctatcgac
     5761 ccgctcgcaa gctaaacacc gatcgccggc atgcgcatcg atcaagtaga tgatggctcg
     5821 acgttcgcaa gctccgggaa tttagcgatc attgcttcac cgggcatcgc tttcgggcgg
     5881 agcccgagtt cctgatagag catgatggca ttagcagccg cgttggcgcg ggcgtgatta
     5941 ttgaaaaatg caaacccctt ttttatcccc ggcgcgcttg tcgcgctctc gattcgttcg
     6001 gcaatctttt taatttcccg gcgagaatat agatagtcat aacgctccca ggattccttc
     6061 ggcctccacc acgccttggc gttgcggccg tgagcgcgga agtaaaaaaa atcgccgaca
     6121 atctcaaggc cctggcgaat ggaagatgaa aatttcggct cgtcgatcag gacgctgccg
     6181 gctctattgg cttccaacag ggctttgatc tcctcgcttt tctcatccca actattatgc
     6241 cggagctcga ccacttttgg ataatcataa aatacttgca gcgttctctc gactcgctcc
     6301 atgttctcgg gcgtgcagtg aaagctcgtc ggatactgca gcagaagcgc cccgagctta
     6361 cccgcctcag cgatgggttc gatgccggcg cggaattgat cgatatcgtc ctgcgtcgga
     6421 ggctgccatt tttcatcggt tatttttcgc ccaatcttta ccggatgagt aaacttctgc
     6481 cacagcttca aggcaaaggt gaagtcggat gaagtcttct tcgcccatgc tgtagtagtc
     6541 gtgatcgagg gaggccggta gaacgtggaa ttgatttcga ctgtgttaaa tatctgtgaa
     6601 tagtagtgga gttcatcgaa gcgtttaggc tttggctcgg gataaaacgc gccggcccaa
     6661 cccgcgggcg gtggtcctgg gtatgaataa cctgaggttc cgattagaag agcccgattc
     6721 acgattctga ctgtcttttt gacggagacg ccctttatct cctttcatac tgacgactct
     6781 gggtatggat tcaatagggg agcagaaggc catagggcag ggcctggaac tgatcagagc
     6841 gtctgacatc tactacgtct cgtatagaag caaaggcgag atctcttgcg agaaaccaaa
     6901 agactgcggt tgataaacga acgcctaacg gcccgtgcag cgcgttggat acacatcaca
     6961 tcatgccgca tatactcgca tccgtttgaa actttaccag caaactttcg agttccgaca
     7021 tatcaacggg ctttaccaga taagcattga agccagcctg ccaggcgcgc tccttatcgt
     7081 gttcctgtcc gtaacctgaa attccgatca gatacacgcg attgagttcc ggatcttggc
     7141 ggagctgccg ggcgactttg taaccgtcaa attccggcag gccgatgtcg cacaatatga
     7201 cgtcaggttg gaatcgtcgc gcggcttgga gaccttcgct cccagtgtgc gccgcttcca
     7261 cagtatggcc ttgacgggtc aggtacacgt tcattgtcct tgccgccgac cgattgtcct
     7321 cgatcaccag gatccggcgg gcggtagggt gaacagaggc agagctgcgc cgtctggctg
     7381 tcggcgcggc ttgatctcga agtggcagcc ggatggtgat ctggaatcca cgccctatgc
     7441 cagcacttga agcgcgcacc tgaccgccat gcagctcgat caggcccttt accagcggca
     7501 atcccaatcc caatccgccc ggacgccgat cgataccatg atcaccctga gtgaatggct
     7561 caaaaacgcg cgcgaggagt tctggttcca tgccgatccc tgtgtcatgc acagtcagaa
     7621 taacctgttt ggcggcagct tcttcctgga gcgcaacggt tatgattccg ccggcgccgg
     7681 tgaatttatc tgcattatgc aggatattgc tgatggtttg cgccaacctg gtccgatctc
     7741 ccatgaccca catcggccgg tcgggtatat cgatctcgag atgcaggttg ttcgtttcaa
     7801 gcttggagcg atagtcctgc atggtttgac gcacaagcga ggaaaaatca cagagagttt
     7861 ggttgagttt catttggccg cgagagattc gtgatacatc aagtaagtca tcaagcagcc
     7921 gggtcatatg ttccacctgc cgatcaacaa tatcgcggag ctcggccagt tccggagatg
     7981 acggttcctg cctgcgcagc aactgtgcgc tgatgcttat gactccgagc gggttccgta
     8041 gctcatggcc caacatcgcc aggtactcat ctttgcggcg atcgacatca cgcaaggact
     8101 cctcggcttc ctgcactctt cgataggcca agctattctg gattgcagtt gcggcggcct
     8161 ccgagatcgc caccaaggtg cgtttgtcca gatccgtaaa gccgccggcg ctggttttat
     8221 tatggatctc gaaaaaaccg atgagctcgc gctgaaagtt tagaataggt atcgacaggg
     8281 cgctgcgcac tccaaatcga gcgtaaacct cgtgcgcggt ccttggctcc ccgtcggcct
     8341 cattggtgag atacggtagt ttctcgctga tgatgtgtcc gggcaatcct tcggatgggc
     8401 gccaacaata atcgaagggg accgctttgc ccttctgtaa atacttgcgg cagaccatcc
     8461 cgtccggcgt gtagaggccg gaaacgccgc tctcgccgtc aacgatccgg atcgcctcgt
     8521 caactaaaac atcgagcagt gtatcgacat cgagtgtgga gttgagccgc tcgctgatac
     8581 gaagcagcct gagcaccgtc tcttcggcca gtttgcgttg cgtgatatcc atggttgcgc
     8641 cgagcatacg gaacggttct ccggcgtttc gcaataccat cgcgttggta aacagccata
     8701 tgatctgccc atccggtcga atcgcccgga attcgagctc gtagggcgcg tcgtgctcca
     8761 gagcgcgttg aagactgtgc aaaaccaaat cgcggtcctc cggatggagc agcgtggcga
     8821 aggcttcgat ggtggtaacg aactcatcgg gccttacgcc gtgaatggca tagagagatt
     8881 cggtccagac cacccggttg gccgcaatgt cccaatccca tacaccgact ttaccggttt
     8941 gcatcgccag ccgcaggcgc gcctcacttt cacgcagaga ttcctcgacg cgtttgcgtt
     9001 cggtgatatc gacgcatgag ccgacgtaac cgatgagctc gccggtatcg gtcactcgcg
     9061 gcgccccacg agtcaagacc cagcggtact caccgtcggc ccgccgcagc cggtattcta
     9121 tttcgaagcg ctcgcggcgc tgaaacgacg cgcgataagt ttcaacacag cgccggcgat
     9181 cttccgggta aatgctctcc gtccagccct cgcccagctc ttgttcaaat cttcgaccgg
     9241 taaaatcgag ccagggctta ttgatatagg tgcattgttg atcgacatcc gataaccaga
     9301 gcagcacggg cgctgtgtcg gccatgctgc ggaagcgctc ctcgctatcc cgcagcgctg
     9361 aatctttagc tcgcaaccgg tctgccgcgg aggagaacgc agctcgaaca tcgtcgacct
     9421 ccgcaacttc ggtgggccaa tgggcactca cgcgttcacc gctggccaaa gcttgagcgg
     9481 tgtgcgccag ctcagaaatc ggccgagcga tccgtctgcc caagacgaca gcaagcgtga
     9541 tcgctaaaat tgcagttgcc gcgactccgg caaccatcag cagcaccgat tgacgcagtg
     9601 ctgcttcaac gccttctttg ggaacccccg aagccaaggt ccaccctgcg agcttcgaac
     9661 ggctatgcgc cgtatagaac cattggccct ccaatccttg agttatggat gagccttcag
     9721 gcgccttgcg cgcttcggcg ctcagcgcct tggagggagg ttgaccgacc catcgttctg
     9781 aatttagggt gcgagcgatg atgatcccgt tttgatccag cagggtcagt gttgcatcgg
     9841 tggccactgg gtagttggac agaaaacgta accagctcgc cggatcgatg acggccacca
     9901 acgtatattg acggccacgg cctgttttgg ctggaacaac aatcgctgtc gtgtagcgtg
     9961 cccccgcacg tccctttaaa atcggcggca cgaacggacg ccctgatttt acaacggctg
    10021 caagatcagt cttatccaac gtggtctccg acaaccgcgt gccgaatgga tatctgagat
    10081 tgatcacctg cttgccggag gcgggatcgc tcaggataat attggcccag gtgctctgcg
    10141 tcgagagagc acgcgcgatc tgttcgtgaa agctaagtag atcattgaat tggagatggg
    10201 gggattccgc cagcatctgg agcgaacgaa tgttgccatc gatctctcga tccagcgcga
    10261 cggtcattcc tcgcacgcgg tccagaaagc gctgctcgag aacctgacgt tgttcacgcc
    10321 agaagaccgc ggtcatcgcg gcggaaaaag ccaggatcgg aaggagggtg ccgagtacaa
    10381 gaaagaggag gtgcgaacga attctcatgg tgccggatga ctgatgatca ttcgcttacg
    10441 atcgttcgca acctgtaaca ttatctcgaa gcgctgcaaa gagaggtcgg gcattctcag
    10501 ctgcatcata tgcgggcaga gtggcgccgg cttgagattc tgtgctgctc gttctctcgc
    10561 cattgtcgtt ccccgccata cctggttcca tttcactccg agctggaaac taatgccgcc
    10621 ttcaatcgca ttgtaaatcc acttgtcaga agttgtaaat aacctggcgc acattccagc
    10681 tggcaatttc tccgaaaata cctcatcctt ttttctaatc gagatgacgc aaaaaagagt
    10741 cgacgttaga gagagcctac ggtcgtcgtt taaggacggg atctttgccg ccttcatggc
    10801 cggcgtcacc gaatattatg caaccccatt agcgcttttt ttaggcgcca cggttcaaca
    10861 ggtaggcttg atcagtgcgg ttccgcagct gctcgcatca gtctcgcagt ttcttgcggt
    10921 ccgaataatc cattggattg gcggccggct taaactgctc gtgcgtttgg ttctatgtca
    10981 agcgttgttg attttttgca tagccattct gccctggctt gaaaccgcgc gacgtgtgga
    11041 gttgttgatt gttctgttga ttttggccgc ggtctgcggc ggcttggctg ggccggcctg
    11101 gggtagcttg atcaccgatt atatcccttc gagtaaaaga ggccgatact tcggctggcg
    11161 caatcggacg gtaggcgcgg tgactttagt tagcatcatc ttatcggggc tggtcctgta
    11221 ttggttcggt gaaatatctt accgggcggg tttttgcctc ctgttcggct cggccgcact
    11281 ggcgcgcttc gcttccggtt attttatcag tcgcatggac gagccgccgc atagaagcga
    11341 cccggcaacc gattttacgt tcttgatgtt cattgctcga tttagggaga gtaacttttt
    11401 gagattcgtg gtttttagcg cctgccttac gtttgcgact tatctatcgg cgccgttttt
    11461 cgccgtcttc atgctgcgcg atctgcaatt cagttatcta acctacatgg gtcttcaggt
    11521 ctgttcctca ttcgcttcgc tggtcgcgct gccgctctgg ggacgacacg cggatctcgt
    11581 cggtaatgtc cgggtgctgc gcctgagttc gttcctagcc gcgctcattc cggcgttttg
    11641 gcttatttct cacgacccgg cttatctgat gttggtccaa atctggggcg ggtttgcttg
    11701 gagcggggta acattgagtg ccgccaattt catttacgac gccgtgactc cgcaaaaacg
    11761 cgtccgctgc attgcgtact tcaacgttat caatggcgct gcgctttttc ttggctccgc
    11821 tctcggcggt cttcttggct ctcaactgcc gccactgcta ggttacggct tgctcagcct
    11881 ttttgctgtt tcgtgcttct gcaggctagg tttttacctg ttgctggcgc gttcctttcg
    11941 cgaggttaga ccgacgcaga aggcgtccac cagagaactt ctcttcagtg tcgtcggcat
    12001 ccgtccgttg atcggccgat cgcaggaata gcatcgttaa ccgacaagcc atctgctcaa
    12061 gcgtgtgctg aaggctggtt aatctctacg gtttgatgtc ttggaggaac cactccgagg
    12121 ggatttcatg acctaagccg tgctcttttg ccgccttgaa tgcgcagtag cccatggcgg
    12181 caaattgaac gccggtgccg acgttattca aaaagaacgt gatctgccgg tcgtttgctc
    12241 tgccaatgac ttttccggcg atgatgtcgc agatttccgg atagcgcgac caatcgcgcg
    12301 gcttttcacg gcgcacaccc ggcatgtcct cgacgatgcc gggcggttga taatgataaa
    12361 tgccgccgaa ttgtgtatgg atggcaacca cgtcgcattt ttccaaagcg ccaatgtctg
    12421 tctcgaattc acgaattgag ttgtagtgca tgccgggctc gagccattcg atgttgacca
    12481 cgggtcgaga ggaattggtc gcgctgcata tgatgtcgca gccttttacc gcttcttcag
    12541 gactgttaac cgcttggccc gcaaccccgg tttctttgga catttgttcc gtgaaatttc
    12601 tacagtgctc gcggttgggg ctaaagactt ttaccttttc gatcggtagt acggccgtca
    12661 tgtaacggta gtgtgcgcgc gcctggccgc cggagccgat caatcccaga gtcttggagt
    12721 ttttgcgcgc catgaatttc atcgccaacg cgctggtgac gccgacacgg gtctgctgga
    12781 caaagccgtc cggcatgatc gctagaagct cgcccgtttc ggcgctgaag agttgtacca
    12841 gaccgagaaa caaacctttg ccggcaagtg gaatcttttc caatcgaaca gtgttgttga
    12901 tcgtttgctc ctggactacg tcggaggtaa cccgcaaagc caggacgccg tacttgccga
    12961 tgcccccgtc caccgactta aagctgtaag tcgaattagg cagcgagtgg ggcaaatagg
    13021 agtggcaagt gggacgattg acggcgcggc tggcagcttg ttcgcgataa gactcctcca
    13081 taacagcaag gctttccttg accgtcatgg ctttttgaat gtcatcattg ctgagaatca
    13141 gggtcacgat ttttcctcca cgcgtcgtta ttttaacgtt atgacttacc cgatctgatt
    13201 cggcttgtcc aattgctgtc ttttgatgtc gagttcgctg ccaagatttc ggtttgatcc
    13261 tgtgcaaccg cgcgcgttat gctgcaagca tgtcctttaa gatttccgtt caggtaaaaa
    13321 cgcaagcgcg gaaagaaaac atcatcaaaa tctcgcaaga ggagtatcaa gtctcagtac
    13381 atgctgcacc gattgcaggg aaggccaacc aatctataat cgacctgctg gcgaaatatt
    13441 tctcggtccc gaaatcctct gtgaagctcc ttcgcggaca atcttcgcga aaaaagttat
    13501 tcgagattgg gtaacgaaca cccgagcctt tggttcggcc cttcgatagt tcgttatcga
    13561 tgctcttcca ccacttacgc gcacgcctaa gagaggctca atgcaagttt taactaaact
    13621 cgccccgcca tgacccgtca cgcgtattgc gtttgcgcaa cggttaaaac cggcttattg
    13681 aagttgctta aagattaacg ataaagcatg tgggaagcga aaccatagga tttacgccgt
    13741 tacattattg gaatcatggg ccactgatgc gatgctccgg cagcattggc tgcccaaaat
    13801 ataatattca aattacatga ctctcattct gaacaatgat gagatcgaaa gcgtgttcac
    13861 gctcgagcaa tgctttgacg tgttggagcc ggcgttacgc gatctcggta ttggcagtgc
    13921 ggttagtatg ccccgacagg atctattggt gcccgggccg cttgatggta gtttccacgg
    13981 cctgaaaaca tcttgcgctt ccttgccgag atcaggtgtg acgacgatgc ggcttacttc
    14041 cgatattctc acctggccgg ttgttggcgg tcggcagcgt cgcgtaaagg ttcctttagc
    14101 ggtgggctcc aagtatgtcg gtctggtgtt tgtctttagc acggccaccg gagaactctt
    14161 ggcgatcctt ccggacggct tcattcaggc cattcgcgtc ggtgttacca atgcgctttc
    14221 tgcgaaatac atggcgcggc ccgattccta tgttatggcg gtctatggct cgggttggca
    14281 agcgcgcccg gctgtgctcg ctatgtgcaa ggtcttgcct atcagcgagg ttcgggtttt
    14341 tagtccaacg aaaagcaatt gcgagaggtt cgtcgctgaa atgcagcaat cgatcgacgc
    14401 cagaatcatc gcggtttcat ccccggaaga agccgcccaa aatgccgatg tggtttcttt
    14461 agcgaccaat gccctcgatc ctttttttcc ggcctcatgg cttgggccag gaatgcatgt
    14521 caccaccgta cggcccagcg agatgatgct ggaagcgttg ttgcgttgtg atcgtatcgc
    14581 tgtttcgaca cgggaagcgg ctcaactctt tacgttgccc ggtcaggaga gcgcggttcc
    14641 ggaatttggc aagggcgact acggtcgcgc ggagctagag aaaacggctg cggactggcg
    14701 cgacaaagca gaactttcag aaataattgc cggcaaagtc aaaggccggg ataaagactc
    14761 ggacgtcact tgcatgctca atcatttggg actcggcctt cagttctcgg ccgcagcggc
    14821 gagagtgtac gagcttgcgc gagagaaggg cttgggaaga gagttgccaa cagagtggtt
    14881 ttcacagaat gagcactcct gaacgtcgag actgatgaaa aagcccgtct gcttggttgc
    14941 actcgccccg cctcggtgca acgtatttag ccctacatct tcgcgcttcg acatgcgcgt
    15001 ctcgcatgtg aaccttagat tatgatcagt ctggatgagc actagacccg aattttgcaa
    15061 ttcagcgtgg cacagcgatc aagggacgaa ccttccggaa cgaaggcaaa aggtctactc
    15121 agtgcggaat cggggctgaa aaacctcctc aaaaacgaag gcccttttca gagacctgct
    15181 agaggttgtc gaaatatata aagcacttat cacgattctc taagaagccg gccgagagaa
    15241 gttaaccggt ctcttattcc cgtcattcgc agcgcttgcc ataggcctgc ttgattactg
    15301 gttacaaccg gcaagccggt atcgatttcg agcgcttcga tcgcttcaaa agtccggaga
    15361 ttaccgcacg agatcagaat cccatcagcc ttcggcgctg aacgaacgac ctctcgagcc
    15421 aggcggtagg tttcctcggg tgtcactttg cccatatcga tactcttctt gaggcccatg
    15481 ccgcgaatcg ccgccacttc gaacccggac ttttccaaga cttcaaccaa tcgagcatcg
    15541 atgtccgtca ggtaaggggt tgcgatcgcc gggcgctgaa tttcgagtgc gcgcagcgcc
    15601 tcgaccacgg cggtaagcga ggtggtcgcc tggatgccgg ttgcggcatg gatacgccgg
    15661 cttaattcgc gatcgtggcc gaagccgcgg aagaacgcga tggcggtgcc ggcctgtaag
    15721 attacatcga cttcggcgga agctagctcc aaggccgcgc gttccacctg ctcggcggtt
    15781 tcgctgaggc cgtccacagt gacatcgtaa agcagcgtgc gcgtttcgac gaggcagacg
    15841 ccttcgggca tggaacggcg ccattcctca gcgcccacgg tttcaggaga aggggagatg
    15901 cgtccgatct ttgcgcgcca accgtacata ttagggaagc aggaggatct tgccggcgga
    15961 tgttcgtgat tctaacaggc ggtgcgcatc cgcggcttgg gaaagcgggt agattcctcc
    16021 aatcctaatc gcaagttttc cttccgccag ccagttaaat agatcgccgg cgcgccaaag
    16081 cacgtcggca tggctggcga cgttgtgtgt caggctgggc cgcgccaacg tcagcgatcc
    16141 cttggcgttt agaatcgaag tgtcgaacgg cggcactggg ccgctagctt ggccgaaaat
    16201 aaccagggtg cccaacgggg cgaggcagtc aaggctctct tcaaatgtag ccttaccaac
    16261 tgaatcatag accgcattga caccttggcc gtcggttaat tccctaatct cggcggcgaa
    16321 atcctgttcg gtataattga tcgtccgatc ggcgccggcc gcttgggcaa gccgtgcttt
    16381 ctcatcgctc gagaccgtgc cgaagaccct cgctccgcgc atctttgcta tttgcactaa
    16441 caacaatcca gttccgccgg ccgcggcgtg gatgagaacc gtgtccttgg gttttactcg
    16501 ataagtgctg tgcgtgagat aatgagccgt catgccttgc agcatgatcg ctgccgcgtc
    16561 ttcaaatgcg atcgactgcg ggatcttgta aaggtgccag gccgggaccg cgtgcaactc
    16621 ggcgtacgag ccgtaaccat tcgagatgcc gtaggcgaca cgatcaccga tctttatctc
    16681 ggtaacattc gcgcctaagt cgacgacagt tccggcggcc tcggcgccag gagtataagg
    16741 gagcgcacgg ccatgatggc caccccagtg aaaccctgaa cgataataga tgtcgagata
    16801 gttgaccccg gcggcagcga ctctgaccaa aacctcgccg gctttcggtt gtggcgcagg
    16861 agcttcttca tagcggagca catccgggcc gccgagctca tgtatacgga ctgctctcat
    16921 caaccattta acacgctgct gaaatgcccc atatgcttcg ttgggctcaa tcgcatcgca
    16981 tcgacgtact gaagagtacg ccttcgctcg tcgattttcc ggccgcctcg tctacgaggt
    17041 cttttgagca gcccgatatc agagtctctc ataacgttgc gcttaattgt ccagaagcgc
    17101 gatcacccgc gccggcgccg attcggtttt ccactttacc ggcagcgcaa taacttgcgc
    17161 cttggagccg atctgatcga gattgataag attctcgatt tgaaaaatca ctctcggaag
    17221 aatggtccgg tgcacgcgca tggcgctctc aagaatttga tcggcgcttt gataatcatt
    17281 tcccttttct tcctgcgcaa aatcaaaacc gactaccgcg ggcttcttcg ccacgatcca
    17341 ttgggcggcc tccttattta aatatggcga atcgttccac cattgagggt ccgtcgtatc
    17401 gtggcctttg ggccagtcgg tcctgagaaa aagcgtgccg ccttgcggaa tctcaatgcc
    17461 ttgttgctgg agcttcttct cggcttcgct tagatcctcg ggcgtaatca cggcgcgggc
    17521 aactcccttt gcggttaggt ccagtatgat tgcctggccg accatcggtt cgagcggaag
    17581 ttgctcgata gaaggcttgt cgcgaaaaaa atgatgcggc gagtcgatgt gggtgccaac
    17641 gtgaacggtc atctgtacga cattggaaac aaaccctttg tcttcccatt tgataatcgg
    17701 tctgagctta aaatagtttt catcgggagc aaacggtgct gagtcgcatt cttggacggt
    17761 catgctgaga tcgacgatgc gcatgctagt tgatcctttc ggcgcgagta atgttgtcat
    17821 atttctccgt ataccggctt aagtcaaccg cgcgggaatc gcagggatct aagccatggc
    17881 cccagcgcat caactctttc aatggaccag agcttctcga caaacctctt cgctcgcttt
    17941 ttcggaatcc gttcgccggc acattcgtag aatttctctt gaatctcttc tcgtgttaga
    18001 ggcttctttg gatgaccttt gcccacagcg gcgagttttc ggtaagtttt cccgctattc
    18061 aattgaattt cgacctcggc ctggcagccg agattgccca ctgaccggag cctcggattt
    18121 tgcacgagac agactcgctt catcacggcg gtaattattt gatcta
//
LOCUS       DASUVS010000029        13394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_8472, whole genome shotgun sequence.
ACCESSION   DASUVS010000029 DASUVS010000000
VERSION     DASUVS010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13394
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8472"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VF182_01705"
     CDS             complement(<1..269)
                     /locus_tag="VF182_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX6435814.1"
                     /translation="MLSAIVLLTTERDKTKRVAEALADTEGVSEVYSVAGRFDLAAIV
                     RASDNDQLADIVTEHVRALPGITSSETLIAFRVYSRHDLERMFSIG"
     gene            complement(323..946)
                     /locus_tag="VF182_01710"
     CDS             complement(323..946)
                     /locus_tag="VF182_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEX6435815.1"
                     /translation="MAAVLLLGLGATAPSRRIVLFLGNSITAGYGLELSQAYPALIQQ
                     RINQEGLNFRVINAGQSGDTSAGGLARMDWLLKNKVDVLVLELGGNDGLRGLPVEVVR
                     KNLQAIIDHARKRYPQIKIVVAGMKMPPNMGGQYSRAFEAMFPDLAKKNNAALIPFIL
                     EDVGGVRQMNLPDGIHPTAKGHEIVAENVWKVLAPVLRSIESPVTRS"
     gene            1094..1807
                     /locus_tag="VF182_01715"
     CDS             1094..1807
                     /locus_tag="VF182_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6435816.1"
                     /translation="MKKTILSVEHLNKLFKSGDQSVTILHDISFAIEEGSACAIVGPS
                     GSGKTTLIAICAGLERPTEGTVVLNGTAMHSASEEELSHVRNHLIGFVFQSFQLLPSL
                     TALENVMVPAEIRGENHARGRAEDLLRQVGLAARTHHYPIQLSGGEQQRVAIARAFMN
                     HPRILFADEPTGNLDSDTAREIVRLIFQLNAQAGTTLVLVTHDRELTEQANRVISLKG
                     GRVLSDTRKETGASHVVNA"
     gene            1824..4328
                     /locus_tag="VF182_01720"
     CDS             1824..4328
                     /locus_tag="VF182_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX6435817.1"
                     /translation="MALRDGRRRIKPMLLAMSCVVLGVAAIVASFSFRDNLQTSVSAR
                     AKSLLGADLVIQSREPFSPEAEDLLQSIGGEQSRQIGFSSMAFFPESGKSRLVEVRAI
                     HGNFPYYGALETEPMSARQDFQSGTNAFVDETVMLQFNAQIGDRLRIGEQEFRIAAKL
                     LKIPGETLAFSLLNPRIYVPLDHFQEAQLIQKGSRVNYRVFFKLPPEMNPDEIAQRLG
                     PELTRLRLESDTVSRRMATISRFTDNLSRYLQLAVFIAVLLAGVGVASAIHVYVKEKT
                     ASVAVLRCIGATPMETLYVYLVQAVLIALAGSMLGAVLGAGVQTLLLVVLKDFLPVDA
                     VISYSISGILAGIGTGLGTTLLFALIPIVSLRKISPLFALRASYESPARARDPVLWAI
                     YLFILVVVFAFALSTTRQWLHGLSFTAAILAAFGLLAFISRGVSMLLKRFMPYFLPFP
                     WRQGLSSLYRPNNQTTAVMLSIGLGAFLLITLYSVRNMLVNQVAERGGRGDANMVLFD
                     VQKDQRQDLAALLRSFKLDSHEEVPIVTLRLAAIKNRPVEEIRDDTNSRIPRWALRRE
                     YRSTYRSYLAGTEKVIEGTWRGKVSADTQPIPISLEKGIAENLGVGLGDELEFELQGV
                     SLPTQVASIREVDWQRVQPNFFAVFPEGVLETAPQFYAVVTRTDSNEVSADLQRAVVE
                     RFPNVSMIDLTLVLNTLDSILGRVASAVRFVAMFTILTGVAVLISAVLSSRSQRLKES
                     ILMKTLGAPRQQIITAIVAEYLFLGAISCATAAMLAALATWGLSFYFLGTIASVSWTP
                     VIVIGLIVTGATVLAGVIGCWGMFGRSTLEAFRLEA"
     gene            complement(4372..4764)
                     /locus_tag="VF182_01725"
     CDS             complement(4372..4764)
                     /locus_tag="VF182_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435818.1"
                     /translation="MHPEIIKEKDTGAIFRIKEHKGCGIRAASYQIRPDKWVPEACFS
                     VYTEAGWRQLWLQSFAHCFSMPDFTCSNKIEADNWAFRMARTVIDKTLPEFDKHEPPA
                     RVKSHLLTCFSKIWGIARHPFSTVYREH"
     gene            5007..5291
                     /locus_tag="VF182_01730"
     CDS             5007..5291
                     /locus_tag="VF182_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435819.1"
                     /translation="MNVPLGRWFQVEAYYKCAADNTGRVIFWQDAQLLFDVANLSTRY
                     GNGDCQWSLNSYSDSITPSPASFYIDDASISSTRLNLIGLAAPTDLKVYQ"
     gene            5432..6226
                     /locus_tag="VF182_01735"
     CDS             5432..6226
                     /locus_tag="VF182_01735"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6435820.1"
                     /translation="MLSLRRISKVFGATDDGSTSLPAVRAVSFEVRDGEFFSIIGPSG
                     CGKSTLLRIIGGLLRTSDGELTVAGELVAGPHPSVAMVFQEESTFPWRTTLANVEFGL
                     QMRGVPEQRRRKTARDMIDLVGLGGFEQRYPSELSGGMKQRVAIARALVLEPKILLMD
                     EPFGALDEQTRIILGEELLKIRDQLRQTIILVTHNINESVQLSDRVMVMTARPGQVKT
                     IVDIDLPHPRDSSIIASERFGRLVALVWSALREESIKSFKQTEQRH"
     gene            6239..7246
                     /locus_tag="VF182_01740"
     CDS             6239..7246
                     /locus_tag="VF182_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435821.1"
                     /translation="MKKRRLVVRQNVTSVLSVLLILFGVTSPAWAAAARTKIVVVVPH
                     RILFTVALPIYIAQEKGFYKENNIDVDAVFTRGGGENVQAVVSGDAQIGLGTGTLAVI
                     SAFVKKAPIKIAAAEITGIDAFWYVQSNTTIRKMDELAGKKIAYSRPGSSTHMAALAI
                     ADQIKAKGLKPLEPVSLGGIPEVYTAVRTGQSDAGWSVPPFQLDLVDKGVLRVVVRGD
                     EITAMKEITPRVHFLNNDFAAKNPEAVRGFFRAQQRALDYMFEHKEDTAKIWTRRAEM
                     KYPESAVLKTWDFYSRAAMTLKPIRGLEATIEDGIRNKFLTQPLTQAEIKSLIDLSFL
                     P"
     gene            7573..8268
                     /locus_tag="VF182_01745"
     CDS             7573..8268
                     /locus_tag="VF182_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6435822.1"
                     /translation="MVVIAESAVRLGWISSFFLAAPTRAAIVLWEQIVHGNALRLTGI
                     TIFEIAACLCIATIVGVFAGFLLWRYKLIGNSYEMFLAALFSSPVILAYPIFLVTFGR
                     TSAAVIALSSIFGLIPIIINTKGAFHNVNPILLQVGASMSLNRKQMFRYILVPAVAPA
                     IFSGFRLALTYILKSVLGLEYVAQVGGVGMWLSDAAFKFEVEEVYAGLLGTIVLSVVF
                     LYAINRIEVFIRK"
     gene            8346..9152
                     /locus_tag="VF182_01750"
     CDS             8346..9152
                     /locus_tag="VF182_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6435823.1"
                     /translation="MRPNNTLFPAQIQTPFSVQALTLVIVIAIWELVGRTGILFDELF
                     PPMLEILQSLWRFVTTPLLLPHLKASVYEVGGALAFAMLTAIPLGILWGSRGSWLEIV
                     EPLILYAAVVPKIVIFPVFILFLGIDVQSKLAVGAIAAFFPISLLTIAGMREVKKVYV
                     DVARTAGASALQIAIYVYLPAIAGQVFTGIRLGMGAAVTGALLAETKIAKAGLGFMIV
                     EYYGQFRIADMYSLLLLIFILAAIANWTLKFLFSILSPLSRASADPGMYF"
     gene            complement(9241..10296)
                     /locus_tag="VF182_01755"
     CDS             complement(9241..10296)
                     /locus_tag="VF182_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435824.1"
                     /translation="MTIPQHNTDSSEFSMGHSISVRRSWIFALVLVLAIFFVAYPSPA
                     QETRRLRAGYTSLSGNMLPLWAARDGGYFKKYGLDFDLISMPSGNEGMSALIAGELEF
                     LAIAGSTTTSAAVGGADVVALATTVERLVAFLVAAPSIQKPEDLKGKSVGISRFGTSI
                     DTGARMAIQHYGLEPIKDVSIVQIGAVASILSAMRGGRIQAAILTYPSIIQARREGYR
                     ELLDIASLGMPYASTGITVRRSYMQQRRDLVTNYVKAIVEAIARVKKDKPFAVEVMSR
                     YLRTTDKEVLEGTYDISVTKYLKRVPFPTVEAFRSVVDELALLNPKAKGQDPKRFYDD
                     SILRELEKSGFISALGG"
     gene            complement(10541..11653)
                     /locus_tag="VF182_01760"
     CDS             complement(10541..11653)
                     /locus_tag="VF182_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6435825.1"
                     /translation="MASRPIIVVEDDRFLRLVQIILDPATPTERLSAFTHFMAHDEPN
                     FKQWCQRLRARLGEIYPAEVRLVADQGDLHPSLSGAHVVIVEGFAIGEKEIVAAGGSL
                     KIVQKYGTIATRIDAAACQQAGVRVLTIRRRANIATAEHAFALILALARKITDTANLI
                     SVTQLRSAGYSPTHFDRGQTPNGNWARINRLRTLYNQQLGVMGLGEIGREVAQRATAF
                     GMRVVYWQRHRLEPDEERRYQVTYCSFDELLATSDCISLHMPGGEGTQGIIGRRELGM
                     IKPGALLINVSQPQLIDRAALIQTLAAGRLSGFGLDTFYDEPGHAHDPLLGFRNVLIT
                     PHLAGSPRFNALQDFEEMLLDIDQTLRNRTEQQTTC"
     gene            complement(11659..12201)
                     /locus_tag="VF182_01765"
     CDS             complement(11659..12201)
                     /locus_tag="VF182_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6435826.1"
                     /translation="MARVRLIDENDAVEHSDLIRKIKGARGGRLINIYRLMIHSPSLA
                     SAWFDLNQAVRYGTEIDGQSREIAVIRVALLNDVEYVLRAHGPAYALKEGLTPAQVEA
                     LADWRASHLFNGKQRALLAYTDAMTREIDVPDPVFDEVRSHFTERQTVELTLLIGAYN
                     MLTRFLKALKVDPEPTSAKS"
     gene            complement(12297..13160)
                     /locus_tag="VF182_01770"
     CDS             complement(12297..13160)
                     /locus_tag="VF182_01770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6435827.1"
                     /translation="MIFPQVSAADVAAVAAHYDDLDLFYRTIWGTHVHHGYWRTGKES
                     TEEAVENLTHLVADRAGIKSGTCVCDVGCGYGGSAKIFAEDYGARVIGITVSKKQYEL
                     AQLFHAGLADVDFMNCDGLANGLPTGAFDAVVSIESSEHMPDKPAFFTEVSRLLRPGG
                     RFVVVAWLAREQPGAAETKYLLEPICAEGRLPSMASAPEYEEMLAAAGFEDLGFEDLT
                     CKVKKTWRVCALRCLTRISTHQFLRKRFFDSTFPNRMFAKTVLRIWLAYTMGSMRYGL
                     FAGRKPWVDKL"
     gene            complement(13179..>13394)
                     /locus_tag="VF182_01775"
     CDS             complement(13179..>13394)
                     /locus_tag="VF182_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435828.1"
                     /translation="RHVLEGAHAANSELLVEDTYPTIDGLKNTLEVQAATDPRAAKFK
                     AEDMVELRFVDEMRKSGFVDKLYGRKK"
BASE COUNT         2928 a         3661 c         3644 g         3161 t
ORIGIN      
        1 ccgatagaaa acatgcgctc gagatcatgt cgtgaataaa cgcggaaggc gatcagggtc
       61 tcggagctgg tgatacccgg caatgcgcgg acatgttcgg tgacaatgtc ggcgagctga
      121 tcattgtctg aagcacggac gattgcggcc agatcaaagc ggcccgccac cgaataaacc
      181 tcgctaactc cttcggtgtc ggcaagcgct tccgcaactc tcttggtttt atctctttct
      241 gtggttagca acacgattgc ggacagcata agtggcctcc ttgatcgata actgcgcacg
      301 actgttggga atcaatttcg tatcagctgc gagttaccgg cgattcgatt gagcgcagca
      361 ccggcgccaa gaccttccaa acgttctccg cgacaatttc atgccccttg gcagtgggat
      421 gaataccatc gggaaggttc atctggcgca cgccgccgac gtcttctaaa atgaatggaa
      481 tcagcgcggc attattcttc tttgcaaggt cgggaaacat cgcctcgaac gcgcggctat
      541 actggccgcc catattgggc ggcatcttca tgcccgcaac gacgatcttg atctgcggat
      601 accgctttct agcgtgatcg atgatcgctt ggagattctt gcggacaact tccacaggca
      661 aaccacgcaa accatcattg ccgcccagct cgagcaccag gacgtcgact ttgttcttca
      721 gcagccaatc cattctggcc aagccgccgg cgctggtatc gccgctttga cccgcgttga
      781 taactctgaa attgagtcct tcttggttga tccgctgctg aatcagcgcc ggataggctt
      841 gcgacaattc aagtccgtag ccggcggtga tgctgttgcc gagaaacaga acgatacgcc
      901 gggagggcgc tgtagcgccg agcccgagga gcagaacagc tgccaagcac ggcaaccata
      961 gcggcgttcc aaatgaggcg cgttgtgctg agcccgttaa aattctagac tgaatgctaa
     1021 ctgatcgatc gatgccctcg cgattcatcc tgccattcct tactccgacg attccaatac
     1081 gaaaaaactg gccatgaaaa aaactatatt aagcgtagag catttgaaca agctttttaa
     1141 gagcggtgat cagtcggtaa ccattcttca cgacatttcc ttcgcaatcg aagagggcag
     1201 tgcctgcgct atcgtcggtc cttccggcag cggcaagacg accttgatcg ccatttgcgc
     1261 cggcctcgag cggccgaccg agggaactgt ggtgttaaac ggcactgcca tgcattcggc
     1321 gagcgaagaa gaattatccc acgttcgaaa ccatcttatc ggatttgttt tccaaagctt
     1381 tcaactgctt ccttcgctca ccgctctgga aaatgtcatg gtgcccgctg aaatccgcgg
     1441 tgaaaatcac gcgcgcggtc gcgccgaaga tctgctgcgt caggttggcc tcgccgctcg
     1501 gacgcatcac tatccgattc agctctccgg cggcgagcag cagcgcgtgg ccattgcccg
     1561 cgcattcatg aatcatccaa gaattctttt tgccgacgaa ccaaccggca atctcgacag
     1621 cgacaccgcc agagagatcg ttcggctgat ttttcagctc aacgcccaag ccggaacaac
     1681 cctggtactg gtaacccatg accgggagct gaccgagcaa gccaatcggg tcatcagctt
     1741 gaaaggcgga agagtgcttt cggacacgcg caaggagacc ggggcgtccc atgtcgtcaa
     1801 tgcttagagg ttggatttgg aggatggctc tcagggatgg ccggcgccgg attaaaccga
     1861 tgctgctggc catgtcgtgc gttgtcctgg gagtcgccgc gatcgtcgcg tcattctcgt
     1921 tccgcgacaa tctccagacc agcgtgagtg cccgggccaa gagtttgctg ggcgcggatc
     1981 tggtgattca aagccgcgaa ccgttctcgc cagaagccga ggacttgctc caatcgatcg
     2041 gcggcgaaca gtcgcggcaa atcggttttt cctcgatggc cttttttccc gaaagcggga
     2101 aatcccggct ggtggaagtg cgtgcgattc acggcaattt cccctactac ggcgctctgg
     2161 agaccgagcc catgtcggca cgccaggatt ttcaaagcgg caccaatgcc ttcgtcgatg
     2221 aaacggtcat gttgcagttc aacgcgcaaa ttggcgatcg gctgcgcatc ggcgagcaag
     2281 agttccggat cgccgcgaag ctgttgaaaa tacccggcga gacgttggct ttctcgctgc
     2341 tcaatccgcg aatctatgtg cccctggatc attttcaaga agcgcaattg atccaaaaag
     2401 gtagccgggt caactatcgg gtttttttca agctgccccc ggaaatgaat cccgatgaaa
     2461 tcgcccaacg tctcggtccg gaactcacgc gcctgcgtct ggaaagtgac accgtgagcc
     2521 ggcgaatggc gacgatctcg aggtttacgg ataacctctc gcgctatttg cagctcgcag
     2581 tatttatcgc ggttcttctt gccggagtcg gcgtcgccag cgctattcat gtctacgtca
     2641 aggaaaagac cgcatcggtg gcggtgttgc ggtgcattgg cgccacgccg atggaaactc
     2701 tctatgttta cctggttcaa gcggtgctga ttgcgctcgc cggatccatg ctcggagcag
     2761 tgctgggtgc cggcgtacaa actctgctgc tggtcgttct taaggatttc ttgccggtgg
     2821 acgcggtgat ctcgtacagc atcagcggaa ttttggctgg catcggcacc ggcctcggaa
     2881 cgacgcttct gtttgccctg atcccgatcg tgtcactgag aaaaatctcg ccattgtttg
     2941 cgttgcgcgc ttcctacgaa agccctgccc gcgctcgcga tcctgttctc tgggcgattt
     3001 atctgtttat tcttgtcgtc gttttcgctt ttgcgttgtc cacgactcgg cagtggttgc
     3061 acggcttgag cttcaccgca gcgattctcg cggcttttgg tttgctcgcc ttcatctctc
     3121 gcggcgtatc gatgctgtta aagaggttta tgccctactt tctaccgttt ccatggcgtc
     3181 aagggctttc gagcctgtac cggcccaata atcaaaccac tgcggtcatg ctctccatcg
     3241 gtctcggcgc attcctattg attacgcttt acagcgttcg caacatgttg gttaaccaag
     3301 tggcggaacg gggtggccgg ggcgacgcca acatggtgct cttcgatgtg caaaaggatc
     3361 aacgtcagga cctcgccgcg ctgttgaggt ccttcaagct cgatagtcat gaagaagtgc
     3421 cgatcgtaac cctgcgactc gccgccatca aaaatcgccc cgtggaagag attcgcgacg
     3481 acacaaactc cagaattccc cgatgggccc tgcggcgtga atatcgatcc acctatcgga
     3541 gctatttggc cggtacggaa aaggtcatcg aaggaacctg gcgcggaaag gtttctgccg
     3601 acactcagcc gatcccgatt tctttagaaa aaggcatcgc cgaaaacctg ggcgttggct
     3661 tgggcgatga actggagttt gagctgcagg gcgtttcttt gccgacccaa gtggccagca
     3721 ttcgcgaggt cgactggcaa cgagtccaac ccaacttttt cgcggttttt cccgagggcg
     3781 tgctggaaac cgcgccgcaa ttttatgcgg tcgtgacccg caccgattcg aacgaagttt
     3841 ccgccgactt gcagcgcgca gtggtggaac gctttccaaa cgtatcgatg attgatctaa
     3901 ccttggtcct caacacgttg gattccattt tgggtcgggt agcaagcgcg gttcgcttcg
     3961 tcgccatgtt tacgattctc accggtgtgg cggtgttgat cagcgcggtg ttgagcagcc
     4021 gtagtcagcg gttgaaagag agcatcttga tgaagacact gggcgcgccg cgacagcaga
     4081 taatcactgc cattgtcgcc gaatacctgt ttctgggcgc gatctcatgc gctaccgcgg
     4141 cgatgttagc agccctggca acctgggggc tcagttttta ttttcttggc accatcgcca
     4201 gcgtttcatg gacgcctgtc attgtcatcg gcctgatagt tacaggggca acggtgcttg
     4261 ccggtgtaat cggatgctgg ggtatgtttg ggcgctcgac cctggaggct ttcaggctag
     4321 aagcttgatg tccggttagt gtcaccgttg cgcaggaccg aatggagcgc ctcagtgttc
     4381 cctatagaca gtcgaaaacg ggtggcgagc gatgccccag attttgctga agcaggtgag
     4441 caggtgggac ttcacccggg ccggtggctc atgtttatcg aattcgggta aggttttatc
     4501 gatcacggtt cgagccatgc ggaaagccca gttatcggcc tcgatcttat tgctgcaggt
     4561 gaagtccggc atcgaaaagc aatgcgcaaa gctctgcagc caaagttgcc gccaacccgc
     4621 ttcagtgtag actgaaaagc atgcctccgg cacccatttg tccggcctga tctgatagga
     4681 agccgcacga atgccacagc ctttgtgttc tttgatgcga aagatcgctc cggtatcttt
     4741 ctctttgatt atttctggat gcatgggcga tctccccgtc ggtgaatcga ttactgttcc
     4801 ggccacagct ttagaattta ggtaagaatc ggcaaaagct ataccaggta attctgacgc
     4861 ctggtggcga gagaatgcgc taaaaccgtc atcgaagagc gcaaaaatgt tcgtttcttc
     4921 atctttaaca tcggcaatcg ttcggacggc aacagtattt ttatctctac gattggcaac
     4981 ggcgcgcttc tagcactcag acaaaggtga atgtgccgtt aggccgctgg ttccaagttg
     5041 aagcttatta caaatgcgct gccgacaata ctggccgggt gatcttttgg caggatgcgc
     5101 agctcttgtt cgacgtggcc aacttatcca cccgctatgg aaatggtgat tgccaatgga
     5161 gcctgaatag ttattcggac agcattacgc ccagtccggc ttcattttat attgacgatg
     5221 ccagtatctc gtcgactcgt ttaaatctca tcgggctcgc agctccgacc gacttgaagg
     5281 tctaccaata actatctccc atttttctac tgttctccct ctcttggtag tttccgagtt
     5341 ctgagactcg gcactcacaa cttgtcgatt ccagtcagat caagggaaag cccttgaccc
     5401 ccaacattga tctgggataa ctgcctccta gatgctgagc cttcgtagaa taagcaaggt
     5461 ctttggcgcg accgatgacg gttcgacttc gctgccggcg gtccgagcgg tatcgttcga
     5521 agttcgtgac ggcgaattct tctcgatcat cggtccatcc gggtgcggca agtcgactct
     5581 gttgagaatc atcggcggat tactgcgcac ctccgacggc gaattaaccg tggctggaga
     5641 actggttgcc ggacctcatc cgtcggtcgc catggtgttt caagaggaat ccacctttcc
     5701 ctggcggact accttggcca acgtcgagtt cggcttgcaa atgcgcgggg ttcccgagca
     5761 acgacgtcgc aagacggcgc gcgatatgat cgatcttgtc ggtctcggcg gcttcgagca
     5821 acgctacccc tccgagctgt ccggcggcat gaagcagcgt gtcgctattg caagggctct
     5881 cgtcttggag cctaagatcc tccttatgga cgagcccttc ggcgctctag acgagcaaac
     5941 aagaatcatt cttggcgagg aacttctcaa gatccgtgat cagctccgcc agaccatcat
     6001 cctggtaacc cataacatca acgagtcggt gcagctctcg gatcgggtga tggtgatgac
     6061 cgcccgcccg ggacaggtca aaacaatcgt tgatatcgac ttaccccatc ctcgagattc
     6121 ctcaataatc gcctcggaac gatttggccg cttggtcgcg ctagtttgga gcgcgctcag
     6181 ggaggaatcg atcaagagct tcaaacagac ggaacaacga cactgaggag gtcaagtgat
     6241 gaagaagcgg cgcttagtcg taagacaaaa tgtcacgtcg gtgttatcgg ttctgctcat
     6301 tcttttcgga gtcacttctc ctgcatgggc cgcggcggcg cgcaccaaaa tcgtcgtcgt
     6361 ggttcctcat cggattctgt ttacggtcgc gctgccgatt tacatcgccc aagagaaagg
     6421 tttttacaaa gaaaacaaca tcgacgttga tgcggttttc acacgcggcg gcggcgagaa
     6481 cgttcaagca gtcgtctcag gcgatgctca gataggatta ggcaccggga cgctggccgt
     6541 gatcagcgct ttcgtgaaga aagcgccgat caagatcgcc gctgctgaga tcaccggcat
     6601 cgacgccttt tggtacgtgc aaagcaatac aacgatccgc aagatggacg agcttgcggg
     6661 caagaaaatt gcctacagcc gtccggggtc gtcgactcac atggcggcgc ttgcgattgc
     6721 cgatcaaatc aaagccaaag gactcaaacc cttagaaccg gtgtcgttag gcggcatacc
     6781 ggaagtttat acggcggtgc gcaccgggca aagcgacgcc ggctggtcgg tcccgccttt
     6841 tcagttggac ctggtggata aaggcgtgct gcgcgtcgtc gtgaggggcg acgagatcac
     6901 ggcgatgaaa gagatcactc cccgggtgca tttcctcaat aatgatttcg cggcgaaaaa
     6961 tcctgaggcg gttcgcggct tttttcgagc gcaacaacgc gcgctcgact atatgttcga
     7021 acacaaagag gacaccgcga agatctggac gcgccgggca gagatgaagt acccggaaag
     7081 cgccgtgctc aaaacctggg atttctacag ccgggcggct atgactctga aaccaattcg
     7141 cggacttgaa gctaccatcg aagacggaat caggaacaaa tttttgacgc agccgctgac
     7201 ccaggcggag atcaaaagcc tgattgacct aagcttcttg ccctaaggct tgtgttcacg
     7261 atgaaccgag cgaagctgca tatttcatct cacggtttta agcccttgac gcagtttgaa
     7321 ttgattccgc agcgggctga ggtattggaa cttggcgatg agattagggc gtcacccccg
     7381 aatgttctta tcgggggtgc agctccgaac tgcctggatt cccgcttaaa gcctgcggga
     7441 atgacgactt cgatagggcg atttaccctt gacgcagcga gctgcagggg agcgatccag
     7501 accgattgaa cggagtttgt tacgcctgaa accacgagag tcaccgcttg gcgtctgggg
     7561 attatcttag gcgtggtcgt catcgccgag tccgcggtgc gactggggtg gatcagcagt
     7621 ttttttctcg ccgctccgac cagggcagcg atcgtgctct gggagcagat cgtccacggc
     7681 aacgcgctca ggttaacggg catcaccatc ttcgagatcg cggcttgctt gtgtatcgcg
     7741 acaatcgtcg gagtgttcgc cggcttctta ctctggcgtt acaaactcat cggcaatagt
     7801 tacgagatgt ttctcgccgc gctgttctcc tcgccggtga ttcttgccta tccgatattt
     7861 ctcgtcacct tcggccggac cagcgcagcc gtgatcgcgc tatcatcgat tttcggtttg
     7921 attccgatca ttatcaatac caaaggagcc ttccataacg tcaatccgat cttgctgcag
     7981 gtcggcgcca gcatgagcct caaccgcaag cagatgtttc ggtatattct cgtgccagcc
     8041 gtggcgccgg caatcttcag cggctttaga ctggcgctca cttacattct taaatccgtt
     8101 ctcggtctcg aatacgttgc gcaggtcggc ggcgtcggca tgtggttgtc cgacgcggct
     8161 ttcaagttcg aagttgaaga ggtctatgcg ggcttgttgg ggacgatcgt gctctcagtt
     8221 gtatttcttt atgcgatcaa tcgtattgag gtttttatac ggaaataacc gtagattcct
     8281 cacggaatcg tgcatcgcgg acttcgcgtc atgcattcgc actatctcaa ctaagaatga
     8341 ctaacatgcg gccaaacaac actttgttcc ccgcacagat ccaaaccccg tttagcgttc
     8401 aagcgctgac actcgtcatt gtcatcgcga tttgggaact cgtcggccgc accggcatac
     8461 tctttgacga actattccca cccatgcttg aaatccttca atccctctgg aggttcgtca
     8521 ctaccccgct gctgttgccg cacctcaaag ccagtgtcta tgaagtcggc ggcgcgctcg
     8581 cgtttgcgat gcttaccgcg attccgctcg ggattctctg gggtagccgg ggcagctggc
     8641 tggaaatcgt cgaaccgttg atcctttatg ccgcggtggt ccccaagatc gtgatctttc
     8701 cggtttttat tttgttcttg ggaatcgacg ttcagtccaa gcttgcggtg ggcgctatcg
     8761 cggcgttctt tccgatttcg ctgctgacaa ttgccgggat gcgcgaagtc aagaaagttt
     8821 acgtcgatgt cgcgcgcact gccggcgcaa gcgctttgca aatcgcgatc tacgtttatt
     8881 tgccggcgat cgccggtcag gtttttaccg gcataagact cggcatgggt gcggcggtga
     8941 ccggcgcgct tttggcggaa accaagattg ccaaagcggg attgggtttt atgatcgtcg
     9001 agtattacgg ccaattccgc atcgccgata tgtattcgct attgctgttg atcttcatcc
     9061 tcgctgcgat cgcaaactgg acattgaaat ttctgttctc gatcttgtcg ccgctttcgc
     9121 gcgccagcgc ggatccggga atgtacttct aatcagggat gaaatctgaa ggcgaaaagg
     9181 tgaatcgaag gcggcgtgtt tgaaccgaac gttgaactgt tgaacataga acagttacta
     9241 tcacccaccg agcgcgctga tgaacccgct cttctcgagt tctctcagga tgctgtcatc
     9301 gtagaatcgt tttgggtctt gtccctttgc tttagggttg agcagcgcta gttcgtcgac
     9361 gaccgaacga aaggcttcga ccgttggaaa gggaacacgc ttgagatact ttgtcacaga
     9421 aatatcgtag gttccttcga gcacttcctt atcggtcgtg cgtaagtacc tgctcatgac
     9481 ctcgacggca aacggtttat ctttcttgac tcgtgcgatg gcttcgacaa tcgccttgac
     9541 atagttagtt accaggtccc ggcgttgttg catgtaactg cgccggacgg taattccggt
     9601 ggacgcgtac ggcattccga gcgaggcgat gtcgagtaat tcacggtagc cttcgcgccg
     9661 cgcttgtatg atcgaaggat aagttaaaat cgccgcttga atacggccgc cgcgcatggc
     9721 gctgaggatc gatgcgacag cgccgatttg cacaatcgaa acgtccttga tcggttccag
     9781 gccgtagtgc tgaattgcca tccgcgcgcc ggtatcgatg gaggtaccga agcggctgat
     9841 acccaccgat tttcctttga ggtcttccgg cttttgtatc gatggagccg ccaccagaaa
     9901 tgccaccaga cgctctaccg tcgtggcaag agcgacgaca tcggcgccgc cgacagcggc
     9961 cgatgtggtc gtgcttccgg caatagccag gaattccaat tcacctgcga tcaacgcgct
    10021 cattccctcg ttgccgctcg gcattgaaat gagatcgaaa tcgagtccgt attttttgaa
    10081 atagccgccg tcgcgcgccg cccacagcgg caacatattc cccgaaagcg aggtatagcc
    10141 ggcgcgaagc cgccgtgtct cctgagccgg agaaggatac gcaacaaaga agattgcaag
    10201 gacgagcacc agcgcgaaaa tccaggaacg gcgaacactg atcgaatgac ccatggaaaa
    10261 ctcggagcta tcggtgttgt gttgcggaat tgtcatcgaa aagcctccat gggcgcagaa
    10321 gatgtgaaac gaaacttcta ctgtcttagg tttgctgccc cggcgtgagc aaatacccgc
    10381 tgcgagcaag ctcccctcta aatcttgacg atccgcaatg agacgttgtg cgactatcaa
    10441 acaaacggct gttaaggtca actcgttcac tgcttaccaa gattccagag taagacggct
    10501 tcgcaggatc tattataaaa cgtatctttg ccggatcggg tcaacaggtt gtttgctgct
    10561 ctgtcctatt cctaagcgtc tgatcaatat caaggagcat ctcttcaaag tcctggagag
    10621 cgttgaagcg cggtgacccg gcgagatgcg gggtgatcag cacattgcga aagccgagaa
    10681 gtggatcgtg cgcgtggccg ggctcatcgt aaaaagtatc caagccaaaa ccgcttaaac
    10741 gtcctgcggc gagagtttgg atcaaggccg cgcgatcgat tagctgaggt tgcgacacgt
    10801 tgatcaataa ggctcccggt ttaatcatac cgagttcacg ccggccgatg atcccttgcg
    10861 tgccttcgcc gcctggcata tgcagactga tgcagtctga agtcgcaagc aactcgtcga
    10921 agctgcagta ggtaacctga taacggcgct cctcatctgg ctctaaccga tggcgttgcc
    10981 agtagacaac gcgcatcccg aacgcggtcg cccgctgcgc cacttctctg ccgatttcgc
    11041 caagacccat cactcccaac tgttggttgt agagagttcg caaacgattg atgcgcgccc
    11101 aatttccatt gggagtttgg cctcgatcga aatgcgtcgg tgaataaccg gcgctgcgca
    11161 gctgggtgac gctgatcagg ttggctgtat cggttatctt gcgagctagg gccaaaatca
    11221 aagcaaacgc atgttcggca gtggcgatgt ttgcccgccg tctaatagta agcacgcgca
    11281 cgcccgcctg ttggcaggca gccgcgtcga tacgagtcgc aatcgtgccg tacttttgaa
    11341 caatcttaag cgaaccgccc gcagcgacaa tttctttctc cccaatcgca aaaccctcga
    11401 cgattacgac atgagcgcct gaaagacttg gatgaagatc gccttgatcc gccaccaggc
    11461 gaacctcggc cggataaatt tcgccgagcc gcgcccgcag gcgttgacac cattgcttaa
    11521 aattgggttc atcatgggcc atgaaatgag tgaaagcaga cagccgttcg gtaggcgttg
    11581 ctggatcgag gatgatctgt accagacgga gaaaacgatc gtcttcgaca acaataatcg
    11641 gacgagaagc catcgagctt agctttttgc gctagtgggt tccggatcga ctttgagcgc
    11701 tttaaggaat cgggtcaaca tgttgtaggc gccgatcagc aatgtcagct cgaccgtttg
    11761 gcgttctgtg aaatgagacc ttacttcatc gaaaactgga tcaggcacat cgatttcacg
    11821 ggtcattgca tccgtataag caagcagggc acgctgtttg ccattgaaca gatgcgaagc
    11881 ccgccagtca gccagtgcct ccacttgcgc cggtgtgagc ccttccttca atgcgtaagc
    11941 cggcccgtgg gcacgcaaca cgtactcgac gtcgttgaga agggcaaccc ggattaccgc
    12001 gatctcgcgg ctttgaccgt cgatttcggt gccatagcgt accgcttggt tcaaatcgaa
    12061 ccaggcgctg gccagcgaag gactatggat cattagacga tagatattga tcagacgccc
    12121 accgcgcgct cctttgattt tacggatcaa gtcggaatgc tctactgcat cgttctcatc
    12181 gattaatcgt acccgcgcca taaagttcct ttaaaaagaa ttcaaatcct ctcatctgta
    12241 agttgtgctg gataagggta actcaacatc ccgctgcaag cttgaaatct ccggatttac
    12301 agcttatcca cccaaggttt acgtccggcg aaaagaccat aacgcatact gcccatcgta
    12361 tacgcaagcc agatgcgtag caccgttttt gcgaacattc ggttgggaaa ggtcgaatcg
    12421 aaaaagcgct ttcgtaaaaa ctgatgcgtg ctgattctcg tcaggcaacg gagcgcgcag
    12481 acgcgccagg tttttttcac tttgcaagtt aaatcctcga atccaagatc ttcaaagccg
    12541 gctgcggcca gcatctcttc atattccggg gccgaagcca tgctcgggag ccgcccttcg
    12601 gcgcagatgg gctccagcaa atatttcgtt tcggctgcgc caggttgctc tcgtgctaac
    12661 caggctacta cgacaaagcg cccgcccgga cgcaataagc ggctgacttc agtgaaaaac
    12721 gccggcttat ccggcatgtg ctcagaactt tcgatagaga ccactgcgtc aaaggcgccg
    12781 gtcggcaagc cgttagcaag cccgtcacaa ttcatgaaat cgacatcggc taaaccggca
    12841 tggaatagtt gagcgagctc gtattgtttt tttgaaacag taattccgat gacacgagcg
    12901 ccgtaatcct cggcgaaaat tttcgccgat ccgccatagc cacagccgac atcgcaaaca
    12961 caagtcccgg atttgatgcc ggcgcggtcg gcgacgagat gggtcagatt ctcgaccgct
    13021 tcttcagtgc tttcttttcc agttcgccaa tagccatgat gaacatgggt gccccagatg
    13081 gtgcgataga agagatcgag atcgtcatag tgcgcggcga ccgctgcaac gtccgcggcc
    13141 gagacctgag gaaagatcat cctgtgacct aacctaagtt acttttttcg gccgtagagc
    13201 ttatcgacaa agccgctctt tctcatttcg tcaacgaaac gcagctcgac catatcttca
    13261 gccttgaatt tggcggcgcg cgggtcggtc gcggcctgaa cctccaaggt atttttgagt
    13321 ccatcaatag tcggataggt atcctcgacg agcagctcgg aattggccgc atgagctcct
    13381 tccaagacat gacg
//
LOCUS       DASUVS010000030         3494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_227276, whole genome shotgun sequence.
ACCESSION   DASUVS010000030 DASUVS010000000
VERSION     DASUVS010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227276"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..1352
                     /locus_tag="VF182_01780"
     CDS             312..1352
                     /locus_tag="VF182_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435829.1"
                     /translation="MQHSSDFPSFYLFLRAGLKAAAFFLVATLLCASPLRAQALKKVP
                     FPFSPIGLNCLPWFVAKDARIFEKHGIDFDPVFIGASSALFNAMLSGAADVAGSGGPA
                     VISNILKGGDIIHITAMVPRFTQSIMVKSEIKKPEDMAGKKIGVSRLGTVTHFALQTL
                     IDGYGIKNVTILQMGGQPEALAGLSRGSVDGAVFSPPYNFQLKKQGYHELASPSDLAK
                     LTPFITNGIVARRSVAEKNNDTVIKTIKGTAEAIKIIQTDREYTKKVMSKWMAMSDAE
                     MVEQSYRFATENYAKEGMVPEAALRGMVKQMVQSNLADPQMASATPLTAYYDNRYVEE
                     VKRSGFFDQLWR"
     gene            complement(1493..2776)
                     /locus_tag="VF182_01785"
     CDS             complement(1493..2776)
                     /locus_tag="VF182_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1736..1738),aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HEX6435830.1"
                     /translation="MRIAHYLNQFFAGVGGEEKAGMPLEARAGAVGPGRLFEQLIGGE
                     AKVVLTLLCGDNYAVENQEQMVAAAVQKIHQANADMLIAGPCFQAGRYGMAAGALCSA
                     VQSQLNIPVLAAMSEENPGTDLYREVMYIVDSGANASKMREAAGKMASLARKLMDNRP
                     IGLPKEENYFPRGFIRDRFVEQTAGQRLVDMVLAKVHGEPFESEMAPTTFLPVPMPPG
                     LKDLTRAKLLLITDGGLVPKGNPDNIEGSAATRWGSYKIDGCDDLKGEDYEISHGGYD
                     PQFVRDDPDRLVPLDVMRELEQSGAIGELHEEFLSTSGLANPLSNTRRMGREMAERVK
                     RLGIDGVILTSTUGTSTRSGAAITTELEKAGIPTVQITSAMPIAKMVGCNRLVLGNGI
                     VHVVGDAKLSTDQEKEVRRQLVRKALKALQADKAE"
     gene            complement(2967..>3494)
                     /locus_tag="VF182_01790"
     CDS             complement(2967..>3494)
                     /locus_tag="VF182_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HEX6435831.1"
                     /translation="GGAETYFYQNHPVITELYRRHHARELNFAGTVATIASADNFDRE
                     RNCRFAANLVRTLLKADAAILTKFGGGVPHTDLSETARLLEAMGIKTAVQVTDLARDQ
                     RVESALLFNFPEVNAIVCIGGNSTRWKVPRVERVIAASAELKEVLGGPLQLESLNVVG
                     VANQQGASRLRAMVY"
BASE COUNT          793 a         1013 c          924 g          764 t
ORIGIN      
        1 ccagcgcccg gaggtcatcg aacgattgaa aaaagtgttg ggaaactgag tcgcgactca
       61 gcacgaattc tcacgttaga aagattcccc gtccttcaaa gagactcaga taaactccgt
      121 gagatatctt gggctatgta atcgcgagag cattgacaat agattagttc aaacggtgaa
      181 tgccttccaa acgtgcaacc tgtttcaaga cggacaccca tcctggcatc gcgtgcttct
      241 taatttggat tcgtcactgg cgagcggaat gtactagacc agcaaccgtc acaaagagag
      301 gaggatccga gatgcaacac tcgtctgatt ttccatcttt ttacttgttt ctgagggccg
      361 gtctcaaagc cgctgccttc tttctagtcg ccacattgct ttgcgccagt ccgctccggg
      421 cgcaggctct caagaaagtt cctttcccat tttcgcccat cggtctgaat tgtcttccgt
      481 ggtttgtcgc caaggacgca cgcatttttg agaaacacgg aattgacttt gacccggtgt
      541 ttatcggcgc ctcttcggcg ctgttcaacg ccatgttatc aggcgcggcg gatgtggccg
      601 gatcgggcgg acccgcggtg atatcaaaca tcctaaaggg cggagatatc atccatatta
      661 ctgcgatggt gccacgtttt acacagtcga tcatggtgaa gtccgaaatc aaaaaaccag
      721 aagatatggc aggcaaaaaa atcggtgtga gccggctcgg caccgtaacg cattttgccc
      781 tgcaaacttt gatcgatggt tacggcatta agaacgtgac gattctccaa atgggcggtc
      841 agccggaagc cttggcgggc ttgagccgcg gctcggtgga cggtgcggtg ttctcaccgc
      901 cgtataactt tcagcttaag aagcagggtt accatgagct cgcttcacca agcgatctcg
      961 cgaagctcac cccgtttatc actaacggta tcgtcgcgcg ccggtcggtt gccgaaaaaa
     1021 ataatgacac cgtgattaag acaatcaagg gcacggcgga ggcgataaaa atcatccaga
     1081 cggacaggga atacactaaa aaggtgatga gtaaatggat ggccatgagt gacgccgaga
     1141 tggtggaaca atcgtatcgc ttcgcgaccg agaattatgc caaggaaggc atggtccccg
     1201 aggcggcgtt gcgcggtatg gtcaagcaga tggtgcagag caatctcgcc gatccgcaaa
     1261 tggcatcggc cacgccgctg accgcgtatt acgacaatcg ctacgtcgaa gaggtgaaaa
     1321 ggtcaggatt cttcgatcaa ttgtggagat aacgtcaaga ctttggtcgc gttgctacta
     1381 gaaccgaccg gaaggtctcg ccgggaaaac ctcggcgatc ggcaggcttc gcccgcttta
     1441 acaccagacg cactgctaca tacccccacc aaccttccat catcggcaaa cattactctg
     1501 ctttgtctgc ctggagcgcc ttgagcgcct tgcgaaccaa ctgccggcgc acttccttct
     1561 cctgatccgt agataacttg gcatcaccca ccacgtggac gataccattg ccgagcacga
     1621 gccgattgca acccaccatc ttggcaatcg gcatcgccga agtaatctgc acggtgggaa
     1681 tccccgcctt ttccagctcc gtggtgatcg cagcgccgct gcgagtgctc gtccctcaag
     1741 tggaggtcaa tatcaccccg tcgataccga gccgtttgac cctctcggcc atctcccggc
     1801 ccatgcgccg cgtattcgac agcggattgg ccaatcccga agtcgaaaga aattcctcgt
     1861 gtagttcgcc gatggcgccg gattgttcga gctcgcgcat aacgtcgagc ggtacgaggc
     1921 gatcgggatc gtcccgaaca aattgcggat catagccgcc gtgcgaaatt tcataatctt
     1981 cgcccttgag gtcatcgcag ccgtcgatct tatacgagcc ccaacgggtt gccgccgagc
     2041 cctcaatgtt atccggattg ccttttggaa ccagcccgcc atcggtgatc aacagcaact
     2101 ttgcccgcgt gagatccttc agtcccggcg gcatgggcac gggcaaaaat gtcgtgggcg
     2161 ccatttccga ttcgaacggc tccccgtgca ctttcgccag caccatgtcg acgagtcgct
     2221 gtcccgccgt ttgctcgaca aatcgatcgc ggatgaagcc gcgaggaaaa tagttttctt
     2281 ctttgggaag gccgatgggc cgattatcca tcagtttgcg cgccaggctc gccatcttcc
     2341 ctgctgcttc gcgcatcttg gacgcattgg cgcccgagtc gacgatgtac atcacttccc
     2401 ggtaaagatc ggttcccgga ttttcttcgc tcatcgctgc cagcacggga atgttcaact
     2461 gcgactgcac tgccgagcag agcgcaccgg ccgccatgcc atagcggccg gcttgaaagc
     2521 acggcccggc aattaacata tcggcgttcg cttgatgaat cttctgaaca gcggctgcga
     2581 ccatctgctc ctggttctcg accgcgtaat tgtcgccgca cagcagggta agcaccacct
     2641 tcgcctcgcc gccaatcagc tgctcgaata gtcttccagg gccaaccgcg cctgcacggg
     2701 cttcgagcgg catgccggct ttttcttccc cgccgactcc ggcaaagaac tggtttaggt
     2761 aatgtgcgat tcgcatgggc agtctcccta tctcgcaaaa tcagttcaaa acgctcagct
     2821 caaaagtgcc agtttctgaa aaaccctgat cacagagtgg tcaaaaagac tcagaggcta
     2881 ggcaagctaa ttacaaggat gaaaacggaa atctgaaagc ggaattcagt tttcagattt
     2941 cataattcat cctttctagc agcctgttaa taaaccatag cgcggagccg tgacgctccc
     3001 tgctgattgg cgacgccaac cacgtttagc gattcgagct gcaaaggccc gccgagcact
     3061 tccttcaact ccgccgaagc ggcgatgact cgctccacac gcggcacctt ccagcgggtg
     3121 ctgttgccgc cgatgcacac gatcgcattc acttccggaa aattgaacag cagcgccgat
     3181 tccaccctct gatcgcgcgc aagatcggta acctgcacgg cggttttgat gcccattgct
     3241 tccaacaagc gcgcggtttc cgacagatcc gtatgcggaa cgccgccgcc gaattttgtc
     3301 aggatcgccg catccgcttt gagaagcgta cggacgaggt tggcggcgaa gcggcagtta
     3361 cgctcgcgat cgaagttgtc ggccgatgca atcgtcgcga cggtgccggc gaagttgagc
     3421 tcgcgcgcat gatggcgccg gtaaagctcg gttatcaccg gatgattctg gtaaaaataa
     3481 gtctccgcgc cgcc
//
LOCUS       DASUVS010000031        72982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_8602, whole genome shotgun sequence.
ACCESSION   DASUVS010000031 DASUVS010000000
VERSION     DASUVS010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 72982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72982
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8602"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VF182_01795"
     CDS             complement(<1..426)
                     /locus_tag="VF182_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX6435832.1"
                     /translation="MRRVLRRRRTRRVLLFLTVGFFLTSTTLYAQADKELIAKGQYLF
                     AIAGGCACHTVPKETYHTGGRAFPIPFGTVFSTNITQDKETGLGDWSERQIYDAINAG
                     RRRDGSRMVPVMPYEAYSGMAQEDLKALIAYMKTLKPVKK"
     gene            complement(430..1359)
                     /locus_tag="VF182_01800"
     CDS             complement(430..1359)
                     /locus_tag="VF182_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEX6435833.1"
                     /translation="MRVAVMGTGAVGGYFGAKLAAAGDDVVFIAPQRHLPALRRKGLR
                     IESPSGDLQVRNGFFTDSPADAGMVDLVLFCVKSYDTAGAAAALAPMLTERTAILSLQ
                     NGIDNPDKLAKLWGSERVLAGVVYIGAQMSAPGVIQHSSGGKIVLGPINDHSTDTALI
                     VEQAISRGGIPCVISAAIEQVQWAKLLWNAAFCAISCLTRATVREIVESAALSNLARL
                     CMKEVQAAARTRGIDLKPELLDETMEFSRGLGDFKPSMLQDLEAGKPLECDALNGIVT
                     RLLEQAGEQAPINRVFHATLSFLDKKLREEALR"
     gene            1475..1672
                     /locus_tag="VF182_01805"
     CDS             1475..1672
                     /locus_tag="VF182_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435834.1"
                     /translation="MEKIDPQHDHLKLEQYCPGQQVMFRGKTYTIQRRTTLASGDAAI
                     VLQGDKEQFVIGADRFFAGVK"
     gene            1720..2712
                     /locus_tag="VF182_01810"
     CDS             1720..2712
                     /locus_tag="VF182_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX6435835.1"
                     /translation="MGKIRLTLACWDYDRTRALQDGSVEVEGIDLTYLPLRVEETFWR
                     MLRYGEFDAAEMSTGSYLMSRDKGAPKLIAIPVFPSKTFRHSCIYINTDSGIRKPADM
                     AGKRVGVPEYQITMATWARGVLQHEYGVSPEKMKWFTGGEEHPGREDKVRHNLPAKID
                     IRPIPAEKTLSGMLEAGEIDAMISAHMPSPFVKRHPKVQRLIPNFREVEQDYFRRTKI
                     FPIMHTVVIREEVYEKNPWIAQSLFKAFDESKKLCQESMYEFSALKYMLAWSIDEMEK
                     EREVLGDDPWAYGLEPNRHVLETLVQYTYEQGLISKPLDVDSLFAKSTLEEFKI"
     gene            2739..3257
                     /locus_tag="VF182_01815"
     CDS             2739..3257
                     /locus_tag="VF182_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435836.1"
                     /translation="MADEKNETLEESKWWIDRGPVKEREKPLGPVPGIIEKWAYELLR
                     GYNQDQAAKNLVDINSRLLKELTVAPGEWGYFSMDVGTYPPEKLAKIKNGLPLPPGAR
                     GLIVDAGYSVRVVITGEPVGERYLDINFTDAPALDYWVDGQLVRERVFSNVGEALKRI
                     RADLPKYLKKTE"
     gene            complement(3337..4515)
                     /gene="ribB"
                     /locus_tag="VF182_01820"
     CDS             complement(3337..4515)
                     /gene="ribB"
                     /locus_tag="VF182_01820"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX6435837.1"
                     /translation="MPLATIEEAIEDIRRGKMVILMDDKDRENEGDLCMAAEKVTPQA
                     INFMATHGRGLICLTLTEERVRLLNLSMMVSENTSPFGTAFTVSIDSVLGITTGISAA
                     DRAKTILDSIDDDAKPQDLVTPGHIFPLRARNGGVLVRAGQTEGSVDLARLAGLKPAG
                     VICEIMKDDGTMARQPDLMRFARKHKLKVVTIADLIQYRLNYDSLVYQAASAPLPTRM
                     GGQFQAIVYNTHVDQSEHLALIKGNISPKEETLVRVHTKYVPGDVFGFELLNTGRVIQ
                     SSMEIIAREGKGVILYLQPEHKGLTPAMVTYPTVHGKQQKNINLSFIYRADFKEYGIG
                     AQILRDLGVRKMRLLTNNKKHLVGLRGYGLEVTSLMPIPRDNPLREAAKQKRTKAKNH
                     "
     gene            5180..5650
                     /locus_tag="VF182_01825"
     CDS             5180..5650
                     /locus_tag="VF182_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435838.1"
                     /translation="MIAKKRFEAQISNNVSNNHSQGRSPFWVKSPGQSNAASEEKAFA
                     VFQSDILIPEQFLATYQRTFRLDPEKVLMLAVLQDAVTCFQENITATCKRKRALYQEA
                     EEWFIAEDKSYLFSFENICEALGFDVSYLRQGLLRWKDSAGETQSSQPVTRAAG"
     gene            5854..6888
                     /locus_tag="VF182_01830"
     CDS             5854..6888
                     /locus_tag="VF182_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEX6435839.1"
                     /translation="MKVGLIGPRGAGKTTIFNMLTGLNAQVGGFAGKEEVHLGVIKVP
                     DPRIDKLSQIFKPRKTTYAEIRFTDFPPSQGEDNLKSNSAMVAQMREVDAIALVLGNY
                     EPDAKPLRELNHLLTEMILADLAIIENRRSRLKKEKARPLEESLLQRAAAALESEESL
                     RNLTFTPDEESLLAGFGFLSRKPLLVLLNQSEDQAGQELDSAYVEELKRRGLQGLALA
                     AKAEMEIAQLDEADRIAFLKEIGIEEPARDRFIRASYRLLDLISFFTSGEDEVRAWTI
                     TQGTVARKAAGKIHSDIERGFIRAEVVAYEDFIAFGSESKCKEAGKLRLEGKDYVVKD
                     GDIVHFRFAV"
     gene            7142..8584
                     /locus_tag="VF182_01835"
     CDS             7142..8584
                     /locus_tag="VF182_01835"
                     /inference="COORDINATES: protein motif:HMM:NF004801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase family protein"
                     /protein_id="HEX6435840.1"
                     /translation="MKTLVQGGFVVGFNGTQHEIIKDGIVVYDGSRIEFVGKDYSQAV
                     DRKIDARGCLVSPGFIDTHFHSGINASDYILNDSTKLDFFAANYLAHGGPTRDGAPSA
                     HLKDVDVGQRFSLIHLLKSGVTTTFEIGGPGGEPERFVDMVDEVGIRTATGPSYKNVS
                     FFHDREGRLEYDWDETRGSEGLKKAVQFAKQFDGACNGRMTAMLFPGHVDSCTPGLLK
                     QTKKAASDAGVGIQIHATINLFEFHTVMQRERCTPIELLQKIGFLDPQVSLSHCIFIS
                     GHSWLAYPYGNDLKIIADSGASVAYSPLKYLKLGITMESFDRYLNAGINMGIGTDTFP
                     KDILSDMRYAALASRVADKSFMSGHPRDVFNAATLGGAKMLGRNDLGRLEKGAKADIA
                     IVNLRDLTFGAVRDPIRSLMENAVSRDVRTVIVDGETLVDNYKYLRLNEEELLDRVQA
                     KGEQVWDSVPKWHWTGKSIDEVVPPSFKMK"
     gene            8844..9554
                     /locus_tag="VF182_01840"
     CDS             8844..9554
                     /locus_tag="VF182_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6435841.1"
                     /translation="MSELSELKKKLAMSSRMLFNAGLVDYSGHISARIPGSDHLVILP
                     HPVSRATVKPDDMVITDFDGKLIEGNYKAPSEVNMHARAYKARNDIQSVAHLHNHMVA
                     TLSMVDKPFYPASSNPGAFFGPGRLPVYMDPALIHTIEQGDAVAKTLGTADAVMLRGH
                     GSMVVAHSIEWVFAGCVDLEEAASRFYKASLLGPVMIYNEDETQRVMKQRRKDSVVQK
                     IWDHNAAKTELAGLMNGI"
     gene            9826..10224
                     /locus_tag="VF182_01845"
     CDS             9826..10224
                     /locus_tag="VF182_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435842.1"
                     /translation="MYDDFLARILEPDVFLPAQFYGTGGLSRQLDGEKRLMIAILKDA
                     VECLNKYRGARSSLGSTQYQNALEWVQDEGTDWLFSFNNICDLLGFDPDYLRKVLIKK
                     EARHANPVRGKIYSFPIGSGAGGGSPAARS"
     gene            complement(10204..11511)
                     /locus_tag="VF182_01850"
     CDS             complement(10204..11511)
                     /locus_tag="VF182_01850"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX6435843.1"
                     /translation="MGPPSEDEETRISREFRREAKKHFTYINNPEIERYIESIGQRIL
                     SVMGPQPFEYRFFVIADPQLNAFAVPGGSVFFYSGLIERAKSTSEIAGVMGHEIVHVK
                     GRHMARHSGPDAISLLSLVGMLLLARSGSGAQAAGVVGQAVSATRQIAYSRQLEMEAD
                     TLGVRYMTAAGYDPAGSLGFLKTLDQERSLNPIDVPAYMLTHPVTQERIANVQLVIRS
                     LGDVQSKSESLDQLKKIQVLIRLERQETDKVIDEYQKLVAQSPSQVMALHLLALAQHK
                     KGQLSLAKTNYEKALALNPEDPGLCRDLGRLYTASGDFGAARTTLDRAIAMEPKEPLN
                     YLFLGELLEKQNDLRGAAGAYLNAQQLSPLWDKPPARLGIVYGNLDRLGDAYYYRARS
                     LLLQDEDERAMAAFEKAIKILGENSARGQTAKEELNNLRARRR"
     gene            11735..14194
                     /locus_tag="VF182_01855"
     CDS             11735..14194
                     /locus_tag="VF182_01855"
                     /inference="COORDINATES: protein motif:HMM:NF025038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6435844.1"
                     /translation="MAHKFEVPAGKLSWHCDLSYLPFTCTAEMTPLEDFIGQDRAIRA
                     IQFGLGVNKPGFNIFVTGLTGTGKTSIVKAFLKKAMAERAPVDANAESPADWCYVYNF
                     TDADRPQIIKMRRGWGRVLKADMEQLIQYLQREGRRVFEGDEFARNRQSLLQELQQKQ
                     QGIMEPLMVEAEKSGFALRMTPSGVALLPIKEGKPMQEADYLALPPEQKKALEDKRGE
                     IGKRVEETLREGKKVEREINQRLEELEKKAGEYLVAMPIAEMREKYRDNLKLLRYFDG
                     VRDHILSSLHRFKAAEPDSAAAQLLPLQMAQPATDPFLPYRVNVIVDNSAIQGPPIIV
                     ETNPSYHNLFGVVEKKPVVGGYVTDFTLIKAGSMSKANGGYIVLYDRDVLANTGVWEA
                     LLRVIKNRELRIEEPAAFFGWAPPQGVRPEPIPTDTKVIMIGDPHLYRALASTDPDFR
                     ETFKVKADFDFQVDRSQQNIIAFACFISDYCSREGLRHFDSSGAARVIEHCSRMVEDQ
                     EKLSTRFSDVVDLLIESDYWAGREKAELVSAKHVEQAIVEKTFRLNLLEKRLQELITD
                     GTILVEVQGSEIGQINGLAVYQMGDFSFGKPSRITAKTYMGRGGLINIEREAKLSGKS
                     HDKGVLILSGYLGGKYAQEKPLSLSASICFEQSYDGVDGDSASSAELYAILSSLSGVP
                     LKQNIAVTGSVNQNGEIQAIGGINRKIEGFYDVCRVKGLTGDQGVLMPESNLRNLMLR
                     ADVVKAAEDEKFHIYAVQSIDEGIEVLTGVAAGARNTAGKFPAGSINALVEERLTHFA
                     ERQRQINAAGVRHDPSLTTET"
     gene            14397..15557
                     /gene="acd"
                     /locus_tag="VF182_01860"
     CDS             14397..15557
                     /gene="acd"
                     /locus_tag="VF182_01860"
                     /EC_number="1.3.99.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaryl-CoA dehydrogenase Acd"
                     /protein_id="HEX6435845.1"
                     /translation="MDFDLTDELVAVRDLAREFAEKEVAPTAAKDDREHAFRGDLVKK
                     MGELGFYGSVIPEKYGGNGMGFLAMVLITEEIARVHSAMRVAINMQIGPALALLQFGT
                     EEQKQQWIPPLVQGESIGCFAITEADAGSDVAAMRTAATRDGDHYVINGTKLWISNGP
                     VADGGLLYAYTDRSQKHRGMTAFYADLRLPGVTRKPLETMGAHCSPLGELTFENFRLP
                     LGNRLGAEGDGFKICMWQLNQTRLNCAAGALGLARAAKEAAINYAKERTQFGQKIGEF
                     QMNQDLIAQMVVHEEAARLLVYRAAWLADQKKPNNLETSFGKYTAAEAAAFAADTAMK
                     ILGAYGYSTEFPVERYYRDAKSYQIVEGSSNVQKMIIAQDALGYRQANRGAS"
     gene            complement(15558..17072)
                     /locus_tag="VF182_01865"
     CDS             complement(15558..17072)
                     /locus_tag="VF182_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX6435846.1"
                     /translation="MTGKTLMVQGTGSSVGKSVLVTALCRIFKQRGLKVAPFKAQNMS
                     LNSFATVDGGEIGRAQAVQAEASGIPASIDMNPVLLKPEADNCSQIIVGGKPLTRVSA
                     ADFPQLKSFLWTAISKSLDRLRERFDVVIIEGAGSPAEINLRDRDLVNMEVALYCDSP
                     VLLAADIDRGGVFASLIGTMQLLEESEKEHIKAFIINKFRGDISLLKPGLDWLENRTG
                     VPVAGVIPYYHDIYIPEEDSMPVEKRRAMKQQRAVDIDVAVIALPHIANFDDFDPLER
                     EAGVGLRYVEAADDLGDPNLIILPGTKTTVSDLAYLKENGRDREILRQVAGGTPIIGI
                     CGGYQMLGLAIHDVDGIESAVPTITGLSLLPVTSRFLTVKATRQISARVLVDRGLFAK
                     AKDFTVRGYEIHMGITTGSSMLNPFEVTQRGSQPCADLEGCMSPDGNVLGTYIHGLFH
                     NDDFRWALLRNLAEKKGKDFSPIIGSFSIDEQYDRLAAHVRDSIDMGLIQRLIA"
     gene            complement(17099..17908)
                     /locus_tag="VF182_01870"
     CDS             complement(17099..17908)
                     /locus_tag="VF182_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6435847.1"
                     /translation="MNRRVAIQAQELSFSYANEPVLTNISFSIGAGELAALIGPNGSG
                     KTTLLRILLGHLRPSAGEVLLDSRRLQVYAPKQLAKTIAYVSQQTGLSFPLTAFELVS
                     LGRFPHASRFRFSSVDNLAVRRALEATHSLHLSERTFTTLSGGEQQKVLIARALAQSA
                     GILLLDEPTVHLDLYHQLQILSALKRQCVEQQTTVLTVLHDVNLISLFADKALLLGSG
                     QLCACGAAAEVITETNIKEFLGVDMKAAEDAETGLRFFIPRDPFRSTVEQT"
     gene            complement(17909..18886)
                     /locus_tag="VF182_01875"
     CDS             complement(17909..18886)
                     /locus_tag="VF182_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6435848.1"
                     /translation="MKLKKPSWLLIGIAVFLSFVLGISVGPMPISFFEMSATDRSILL
                     NLRLPRSLSAFFVGGGLALAGAIFQGLFRNPLADSYLLGVSSGASLGAAAAVVLGLAS
                     PVASFAAISFFAFLGSLMALGLVFYLGHLNGGFSIFRLLLSGLGVGLFLSSLVAIFML
                     VAGEELKSLIFFFLGGFSSSSWDTTWVSLGLVPLALIFACYFASELNVLLLGEETASS
                     SGIRTDRIQLLYAILAALLTSISVSVAGLIGFVGLIVPHMIRLIFGADHRTLFPLTFF
                     SGGVFLVLCDTAARIIIAPAELPVGIVTSLLGVPIFLYLIGKEPAGYTF"
     gene            complement(18883..19839)
                     /locus_tag="VF182_01880"
     CDS             complement(18883..19839)
                     /locus_tag="VF182_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX6435849.1"
                     /translation="MRTRPPLHNVGLRTATIAVALFTMLLLKGSSAVAAERIVIDDLG
                     FKVAIKQNVQRIVSLVPTNSEMVCLLVCDRLKGGTRYDESPQELKSRVDHKKIDIVGG
                     GFDANLEKIVRLQPDLILTNGPSQQRFAVPLKNMGYPVLSLWPHDMTSLRKDFLLLGQ
                     LLGREHKAEQILDETQRKLLEIEASARNHRRQRVYLQMWTEPLITVGRSSFPHWLVAA
                     AGGINIFGDMPFDSGQISLESIIQRNPEVLIFLGDQKPFAERIKTQAGWSVIDGVRNE
                     RFCFIEERDIRRSVQFVNGLKKIHGCLVSDTRNASRPAPEAN"
     gene            complement(19841..21901)
                     /locus_tag="VF182_01885"
     CDS             complement(19841..21901)
                     /locus_tag="VF182_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX6435850.1"
                     /translation="MKALFCFVVLVLFAAALPAAEPTDKTLPPVLVTATRLRDIDQPV
                     AQIPGKVIVITTEEIEELGASTVQEVLQHQTGIVLYDAIGNEFQASVDLRGFNGQPVP
                     GTSVFVDGVRVNEPDFNSINFDLIPIESIERLEILPGTATVFGRNALGGVINITTKRG
                     RRDRPHIGFDLGAGSYARQRYSLSSDGPLALKNLDYLLNVSRELSDGFRDGTGARISR
                     LFGKAGYQFSDSTDATLSYTHVQDHLKQAGSLPGSFLRAGRRRHNLTPGDFADSNLDL
                     IGLNVRQKLPAAFSLAVNGFLRNHDQQIFVRGLSSNSNLDTDTLSAGGTIQISHEGTI
                     LAKKNVAATGLEYGHNRFETTSASAFICCPGDFITEQSTRENVVGVFFTNSLNIFDSL
                     ILNAGFRYDWDRLNFTGKTDPTLSGTKEFKRFSPKTGLTYTPVENLSLYFNYSQGVRV
                     PTVQEIFALGPFGSNLNLEPMRSRNFETGFKAKPAEWLEAAISLFYTPVRDEILFIAT
                     DPLNPFSGRNENISRTLRQGVEVSFKLRPDKWIDAFINYTLTKATFETDFFIPGINAV
                     DPPRPVTKGDELPLVPRHRVGLGVNVRPLDRLTFSLFGNYVGEQYQLRDEPNAAKKLA
                     DYFVLNSRIAYEWREWLAHVTLNNLTDRKYSTSGILVGAPFNESFRVPAPGINVFAGL
                     SFRY"
     regulatory      complement(21927..22283)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     regulatory      complement(22439..22639)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(22726..23619)
                     /locus_tag="VF182_01890"
     CDS             complement(22726..23619)
                     /locus_tag="VF182_01890"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX6435851.1"
                     /translation="MSDLSEMEVFIPLFPVVPVFVLITIGFCFAHWKKITLFPITELI
                     VYLGTPSLAFTSLASKPILLNDIFVLAAGVLLIFAGVGLLIRLYFSLFRFSSRGFALP
                     VLFMNAGNMGIPLALFAFGQAGMQRATLMFVIITLLQYSLGIYLLSGRGNWIEIFRLP
                     LIYATFAGLMLNLNRMTIPDLLFQPIAMLGQATIPIMLVSLGYRLHEFQSFKWRHAVG
                     GALVRICGGFLTASIVVQLIAAEGVNRQVLLLYGALPAAVINFVLTEKYGKDPELAAS
                     IVVLSTFISVVTIPTVFWLIL"
     gene            complement(23620..24330)
                     /locus_tag="VF182_01895"
     CDS             complement(23620..24330)
                     /locus_tag="VF182_01895"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX6435852.1"
                     /translation="MKSIVAALKFLTILSRLIHSQPTPAVVGTASVLFPVVGLVIGVS
                     LVLLARALQNYLDAELLSTTIVAFLVLVTGGVHLEGLKKTFDAPALRQPAAGSNSSSS
                     AVGIIAIVFVILFKIKSLDILEERLTVALLLIPVMARWTMLVFIYGSYRHSEGEAGII
                     AANMRFWHFVFATIATLAPATYFLGRTALWIGLCLSILALFCRALFHKRTGALTKDNF
                     GAVVEASEALSLVLLATL"
     gene            24565..26994
                     /gene="gyrB"
                     /locus_tag="VF182_01900"
     CDS             24565..26994
                     /gene="gyrB"
                     /locus_tag="VF182_01900"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX6435853.1"
                     /translation="MAQSKPPQQNDYKADNIKVLEGLEAVRKRPGMYIGDTSERGLHH
                     LVYEVVDNSIDEALAGHCDNIEVTIHIDNSVTVVDNGRGIPVDIHSTEGVSAAEVVLT
                     KLHAGGKFDKSSYKVSGGLHGVGISVVNALSESLEIEIKRDGRVYQQSYRRGVPQDRL
                     KEVGRSKERGTKVTFTPDTEIFETVEFSFDILSQRLRELAFLNSGVKITIEDERSQKK
                     HEFLYKGGLLSFVEHLNRAKTAIHPKVVHFAGERDGVEVEIAMQWNDGYSENVFSFAN
                     NINTAEGGTHLIGFKSALTRTVNSYAIDIGQLKKDDANLQGEDVREGLTAVVSVKVPE
                     PQFEGQTKTKLGNSEVKGIVEALVNEKFSSYLSEHPADAKKIISKGVEAARVREATRK
                     AKDLARRKGALDSGSLPGKLADCQERDPALSEIYLVEGDSAGGSAKQGRDRRNQAILP
                     LKGKILNVEKARFDKMLSSQEIRLMITALGTGVKEDYDSSKLRYHSIIIMTDADVDGS
                     HIRTLLLTFFYRQMPDLVEKGHLFIAQPPLYKIKRGKQERYLKDEATLEDHLIELGTE
                     NIRVRATNDGNGLAGQSLQSFVKKVARWEKLMALITRKRKNRAVVEGLLLEEQFSEDT
                     LKDEKALRALESRLVSYINLTAPDQAPVSCALEEDPEHNCFKLICATRTNGSRSQTII
                     DREFLISAEYKEIKRLFSELTSIGVPPYAVENGEQKAQLRSLTEVLRYIMEGGKKGQY
                     IQRYKGLGEMNPDQLWETTMNPETRILLQVRVEDAVEANDIFSTLMGDEVEPRRKFIE
                     DHALTVKNLDV"
     gene            27076..29529
                     /gene="gyrA"
                     /locus_tag="VF182_01905"
     CDS             27076..29529
                     /gene="gyrA"
                     /locus_tag="VF182_01905"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011936952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX6435854.1"
                     /translation="MEATFKSQKVPVYIEDEMRQSYMDYAMSVIVGRALPDVRDGLKP
                     VHRRVLYAMYDMGNTWDKPYKKSARLVGDIIGKYHPHGDTAVYDTIVRMAQDFVMRYP
                     FIDGQGNFGSVDGDSPAAMRYTEIRMARLAEEILADIEKDTVDFIPNYDDSLKEPAVL
                     PSRIPNLLVNGASGIAVGMATNIPPHNIGEIVDGLVAMVENPGITIKELMKYIPGPDF
                     PTAGFIHGKEPIVQAYSDGKGIVQMRGKAFTETLKRTGKEQIIISEIPFMVNKVRLIE
                     QIAALVQEKKIDGISDLRDESDREGMRIVIELKRDAVPEVIINQLYKHTSLQESFGIN
                     MLAIVDGRPKLLNLRDVLRAFLDHRKEVVTRRTAFDLRKAEERLHILEGLRIALDNLD
                     AVITLIRNANDPKAAKDGLVASFGLSELQAQAILDMRLQRLTGLERDKILQEHKETVE
                     LIAKLLAILADEKEIYKIIVTELKDIKEKFGDERRTQIVARSEEISIEDMIVDEEMAV
                     TISHEGYIKRNPVTLYRAQRRGGKGKIGTTTKEEDFVEYLIIASMHSYILFFTTTGKV
                     YWVKVHELPQASRAAKGKPIVNLLNLEPGERVSAFLSVREFVEGRFVVFATKKGAVKK
                     TDLMLYSNPRASGIRAIGLEDGDEVIGVRLTDGQQELILSTLDGQSIRFKEDQVRPTG
                     RGTYGVLGMRLDSGDAVVSMEMLNRGASILTVSEKGYGKRTEMEEYRLQSRGGKGIIT
                     MKATEKTGRIVGGQQVTDDDQLMLVTNNGKIIRLRMRDIRVIGRNTQGVRLIDVEEGE
                     RVVSLARLAEKEEDEEEPE"
     gene            29612..30619
                     /locus_tag="VF182_01910"
     CDS             29612..30619
                     /locus_tag="VF182_01910"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX6435855.1"
                     /translation="MGKLISIIGAGGWGTALATVLGQKRARVNLWCHGAQTYRELKET
                     RQNRSYLSEIMIPDTVNVTRSLVEAVTDAELVCCVVPSHAVRPVISEAASHINSKAVI
                     VCGTKGLEEETSKTMGEVLLEIFGEGAAQRLAFISGPTFAIEVARGLPAALTVAAYSE
                     NIAAEIQALLSTSSLRAYTSKDVIGVQMGGVIKNVIAIAAGISDGLGLGHNARAALIT
                     RGLAEMTRLAIRMGAEPLTLAGLPGVGDLLLTCTGGLSRNRQVGLQIGAGKSLEEISH
                     GSRMVAEGVRNARSVYFLAQKLGVEMPIVEQMYRILHEGKKPAEGVQQLMQRSLKAER
                     A"
     gene            30827..30988
                     /gene="rpmH"
                     /locus_tag="VF182_01915"
     CDS             30827..30988
                     /gene="rpmH"
                     /locus_tag="VF182_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX6435856.1"
                     /translation="MKRTYQPSNIKRKRTHGFLARMETPGGRNVLRRRRAKGRKKLTV
                     TVPPKRART"
     gene            30991..31356
                     /gene="rnpA"
                     /locus_tag="VF182_01920"
     CDS             30991..31356
                     /gene="rnpA"
                     /locus_tag="VF182_01920"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX6435857.1"
                     /translation="MPIAVGAGFPKQARLRKRPEFIHLSRSGKKQHTANFVIITRETD
                     RSEARLGITVSSKVGNAVVRNRMKRLLRECFRQWNHQIVPARDILIIAKPGAAKLSFL
                     QVASEISRYVTDSRICKRS"
     gene            31377..31574
                     /gene="yidD"
                     /locus_tag="VF182_01925"
     CDS             31377..31574
                     /gene="yidD"
                     /locus_tag="VF182_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX6435858.1"
                     /translation="MIGILGVYHRFISPFLPQACRFFPSCSVYVAQSIYKYGLIKGAS
                     LGVKRLSRCHPWHPGGYDPVH"
     gene            31586..33202
                     /gene="yidC"
                     /locus_tag="VF182_01930"
     CDS             31586..33202
                     /gene="yidC"
                     /locus_tag="VF182_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX6435859.1"
                     /translation="MEKRAFLAIALSLLVLVLYQEWISRQYPAPPPQSPAEQKTEAGK
                     AQTADSVTPLPPVEIKPVPTPAGKPIEDIRIETDNYIALFTTHGGRLKSFKFKHYHAT
                     SDPNSPPFEMIATVPGVAYPLGVKWSSASPADDASVIYGVDGGDLKLSGEAKGTLTFQ
                     GTAPDGATVTKKLTFSGAQYPIELEISARSANGSPLAPATLLTAPDQHGKPNPEAPFE
                     GLLTVVDNKIKREHVTDELRKGKNFSGQLSWAGFGFTYFMMALVPENGAGYRVDVQQS
                     GPALILTVAEPTGKPNPSQYTLFVGPKELSILESIGKGMEKAIDFGYFGFISIPFLYV
                     LHFFHQFTRSYGIDIILLTVLLKLLTAPLTHKSYASMKQMQKLQPQMERLKEKYKDDR
                     EKLNKEIMELYRRNGVNPLGGCLPMVVQFPVFIGLYNALYIPIELRHAPFLWINDLSK
                     PDWQSLPFEFAGWHLGVPVLTILMGASMFMQQWMTPSAGDPNQRKLMLMMPLIFTFMF
                     VTFPAGLTIYWLVNNVLSIAQQWWINRSVK"
     gene            33224..34036
                     /gene="jag"
                     /locus_tag="VF182_01935"
     CDS             33224..34036
                     /gene="jag"
                     /locus_tag="VF182_01935"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEX6435860.1"
                     /translation="MDYVETEGDSIDQAIENALTSLGVERERISIEILSEGKRGILGF
                     GTQKARVRASLRRPAVETNHLEPAAEPDVVASISPEPLSDAETAAIGEKAKVVLSEIL
                     KRMGIEAAVQTKSGDKADEIVLEVISDDSGLLIGRKGQTLEALQYLVTRIAGDRQGSE
                     GPHILVDIENYRQRRKKSLEDMALRLGEKAKRQRKTVTVDALSAADRRIVHAALQDDP
                     WITTKSLGQGSYRRLLIIPEGDRKRKEELKSVDKQGSRSQSNIDRPGPARTS"
     gene            34130..35224
                     /gene="ispG"
                     /locus_tag="VF182_01940"
                     /gene_synonym="gcpE"
     CDS             34130..35224
                     /gene="ispG"
                     /locus_tag="VF182_01940"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX6435861.1"
                     /translation="MKRRATRQIQLGKVKVGGDAPITVQSMTKTDTSDVRATVHQIWS
                     LEAAGCDIVRVAVPVRAAAEQLAEIKKKIRIPLVADIHFNYKLALIALEQGVDGLRLN
                     PGNIGARRYVEEVVKLAAERKTPIRIGVNAGSLEKDLLDKAGGPTVEGMVESGLRHIR
                     ILEDLNFSQIKISLKASDPLMMIEAYRMLADKVDYPFHLGVTEAGTPTVGTIKSAVGL
                     GTLLAEGIGDTIRVSLAADPVEEVRVGAEILKSLGLKKEGLTFVACPSCGRADVDLVG
                     LAKAVEERMIPYNNLNIHVAVMGCEVNGPGEARAADLGVAGGKGIGLLFKKGQVIRKV
                     PEARIVDALMEEVQKFAEEKKAEQSAPADD"
     gene            35266..36999
                     /locus_tag="VF182_01945"
     CDS             35266..36999
                     /locus_tag="VF182_01945"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX6435862.1"
                     /translation="MRWSQILIPTLKEDPSDAEVISHKLLVRGGFIRQVSRGIYDYFP
                     LALKVIRKIENIVREEMDRAGAQELLMPIASPAELWQESGRWDVYGKELIRFKDRNDR
                     DFCLGPTHEEIVTDLVRRAVRSYRELPFNLYQIQTKFRDEVRPRFGLMRGREFIMKDA
                     YSFHTDIDDCKREYENMVVAYRRIFDRCGLKYRPVEADTGAIGGSLSHEFQVLAESGE
                     DAIVSCNNCDYAANLEKAAVRPQRAAARDLNIEAPGLQKVSTPGKKSVAEVAEFLKLA
                     PEKFIKTMIYQVNGDELVAALVRGDHEINQVKLQAALGCREIALASETAVVAATGVTP
                     GYLGPIGLKLRTIADLSVQSMRGAVTGANEPDAHYIEVDQERDFTPTTFADIRLALAG
                     DHCPRCDQGRLEAHRGIEVGQVFYLGKKYSELMGATYLDAEGRERPIEMGCYGIGISR
                     LVAAAVEQNHDDNGIIWPLSIAPFQVLLLPINYTDNATREAADVLYGELRQRGLEVLL
                     DDRDERPGVKFKDGDLIGIPLRITIGAKGLQKGSLELRWRREGKSIDIPVTGAAQTIH
                     SHLSKSFQQTT"
     gene            36996..37769
                     /gene="pyrF"
                     /locus_tag="VF182_01950"
     CDS             36996..37769
                     /gene="pyrF"
                     /locus_tag="VF182_01950"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX6435863.1"
                     /translation="MTSKLELLRARRPTRERLIVALDVDDLNQAKDLVQALASEVGMF
                     KVGKQLFTHAGPQAVRLIQELGGEVFLDLKFHDIPNTVAKAAVEATRMGVKMFNVHAS
                     GSLEMMRMTVKEVRRVCRQEKRRRPIMLAVTVLTSLNQDDLKRVGIDGRVAAQVVRLA
                     LLTNEAGMDGVVASPHEVRAIRDCCGRRFIIVTPGIRPADSSRNDQQRIMTPAAAVKA
                     SVDYIVVGRPILEAKDPVAAARSIVTEMKSSSKEPVGVS"
     gene            complement(37819..38619)
                     /gene="thiD"
                     /locus_tag="VF182_01955"
     CDS             complement(37819..38619)
                     /gene="thiD"
                     /locus_tag="VF182_01955"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX6435864.1"
                     /translation="MKTQIYKALTIAGSDSGAGAGIQADLKTFAALGVYGTSAITAIT
                     AQNTVGVTAIVELSPKIVAAQIDAVIDDIGAHAVKTGMLANAAIIECIAEKLRAHKLA
                     NVVVDPVMVATSGDLLLEKNAVSALRSQLLPIATVVTPNILEANQLAGLTIANARDIR
                     RAAQRIVDSGAQSVVIKGGHRRGPATDLFFDGKKFRQFRTPRIRTIHTHGTGCTLSAA
                     IAAYLAKGEKLESAIMHAKTYITAAIRKGFPVGSGHGPVHHFFRYWGK"
     gene            38973..39110
                     /locus_tag="VF182_01960"
     CDS             38973..39110
                     /locus_tag="VF182_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435865.1"
                     /translation="MNEFLLLALVAIYLTISYPPKSKQPNSNDSEWTRRLFAFIGKKR
                     A"
     gene            39398..41113
                     /locus_tag="VF182_01965"
     CDS             39398..41113
                     /locus_tag="VF182_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEX6435866.1"
                     /translation="MSLKEKASPASFLNYTPVPLAFGTSGLRGLVKDITDLEAYINVK
                     GALRYLLSIGDMAAPRKVVLAGDLRPSTDRIMRACMQAMLDCGCSVENAGKIPTPALI
                     SRAMASRCAGVMVSGSHIPFDRNGIKINKSIGEILKADEPGILREVERVREEEYNRTA
                     ATSAFNADGMLKQAPNLPPLDRRAEEAYVRRYTDSFNNNGLSGLRVLIYQHSAVGRDI
                     LPRILTELGAAVIAAGRSDRFIPIDTENISGEQLDDLEKMISGVEADGQPFEAVLSTD
                     GDSDRPLVVAVLPTAQVPRGGRRVRFLPGDLVGIITAEYLGADAVAVPVSANDAVERR
                     MDARGILLRKTKIGSPYVVAALDEIRLTGKRGRVVGWEANGGFLTGSDIRLAAGTLSA
                     LPTRDSTLPILANLFAAAERNISLAELWNLLPARFGCAGLVDNFPLAASQAILTKLIP
                     PGGLIEVEFDAAGDVFDRSTAANTAAPLAPSLARDWRHAKMTLNRFFTPAQGFDDIAR
                     INVLDGVRIYFRNGDVAHVRPSGNAPQLRLYANSDSQARAEEIRELALREPDGILRQL
                     ERAFT"
     gene            complement(41140..42183)
                     /locus_tag="VF182_01970"
     CDS             complement(41140..42183)
                     /locus_tag="VF182_01970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6435867.1"
                     /translation="MRFGTFSYNQARPWVSEKQAFEELLEQILLTERLGFDEAWFAEH
                     HHSDYGLIASPNLMIASLAHRTKRLRLGNLVNVLPLYDPMRLAEECGMLDILTGGRLN
                     VGLGRGVPRDDLKHGLDRDTAQARFEEGIEIMMRSWTEETFSYSGKAWNYVDIVCRPR
                     PLQRPHPPIYYGATSPDSPAMVARRGWNLALSRQPLANCAQAIRSYRDERTKHKNLSG
                     NGDAIMVRDIYVAETDEQAWEEAVPQLVRFWQLATDNVWRGDSVSADDLPKFTERFPY
                     FPGGLTVKRLDQWGTSLIGSPQTVLAKARAMIETAKPDSLVGMFSFGGLSHDQVTRSI
                     ELFATKVRPALGL"
     gene            complement(42385..42864)
                     /locus_tag="VF182_01975"
     CDS             complement(42385..42864)
                     /locus_tag="VF182_01975"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX6435868.1"
                     /translation="MKSKGHFYFGTFLILVSGYAIYAASQWSFKTGFFPLAVAIPLLG
                     LTLLHCYLELFGAPERNKGPAVEAEFSNEVPPEVARRRVITMFSWMAAFILAVYLVGF
                     PWTVPLFVFFFLKFQSEVSWTKSIALTGITGMLFYGLFQWLVRIQFEAGAIQSWLGM"
     gene            complement(42864..44369)
                     /locus_tag="VF182_01980"
     CDS             complement(42864..44369)
                     /locus_tag="VF182_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6435869.1"
                     /translation="MFEALIDGLSLVLQWKAFSLMLLGMGLGFCVGLLPGIGGAATLA
                     LMIPFVFKMSPAEAFAFLLGMHSVAATTGDITSILFGVPGEGLSAATVVDGYPMAKNG
                     EAGRALGAALMSSLVGALIGAVALALAIPVVRPLVLTFGSPELFFLSMIGIACITSLS
                     GLGLRGQIRGFAMGLFGLLLSTIGQERQSGSLRFDMGLMYLWEGLDLVPVLVGIFAIP
                     ELVDLAVRGTSIAGDRPAENLGRGVMEGIKDTFRHFWLVVRCSAVGVFVGVMPGAGGG
                     VAQWMAYAHAVQSAKDSKDRERFGKGDVRGVLGPGAANNSKEGGDLVPTIAFGVPGSG
                     AMAILLGAFMIMGLVPGPDMLSKHLAVTYSMVWTLAIANIITVLLSLAVLNQLAKVTY
                     IRGGVIIPFVLLLVFVGSYTANGQLSDLIVTFCFGVLGYLMVIWGWPRPPLVLGLVLG
                     KLVETYLFISVARYGFDWLTHPLVIVLMALMVLVIVYPFLREKPAWSGDGD"
     gene            44627..45430
                     /locus_tag="VF182_01985"
     CDS             44627..45430
                     /locus_tag="VF182_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435870.1"
                     /translation="MAKLVLAAGVPHPPRLVYEMAQAPGKTRGEALMKQVREQVEKAE
                     PDLIIEVDSDHFVNFFYNNLPSFCVGMAEEAEGPQETWCPMPRYTVRGNVPLAKALFK
                     YGVHNRFDLAAAHELRLDHSLVVPLHFLNPGMELPVVPIYTNGFASPLPLASRCLALG
                     RMLRKFIEAWEGKERIALIASGCFAMDVGGPLRGWTDTEWAETISSLLLKGRYRTLAH
                     RATEDRINAAGNNSGELLNWITVSGAVGRTRPLFLEGDEGNGYAVWKFE"
     gene            45614..45931
                     /locus_tag="VF182_01990"
     CDS             45614..45931
                     /locus_tag="VF182_01990"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435871.1"
                     /translation="MSVFQVNKLLYLTDNDPELRKRMLADPEAVLREFRLTAEERDAL
                     TSGAVGKLYQMGVHTFLLNHLYRYQLFGVNRDNYLERIREGMPYDPRFELGNMPVQRF
                     INK"
     gene            complement(46061..47074)
                     /locus_tag="VF182_01995"
     CDS             complement(46061..47074)
                     /locus_tag="VF182_01995"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6435872.1"
                     /translation="MRYHKALRALTCLSWLVVCVAPAAGQANFYEGKTINVVIGAKSG
                     SLAIAAQIVAHHLGKHIPGKPAVIVQQMPGAAHLLATNSVFNSAKPDGLTILAANPNV
                     AIAQLSKVEQVRFDVRKFQWLGSSGADGSAFSIRSDLPYKTFDELKKADRELVAGTTG
                     PGSNAHDIPLLLKEFAGVKLKLVSGYPANSDVLLAIERKEVDSWSALATTIKLAADRG
                     VVRPLVRGRVAIKGFENLPVDEDLATSPLGKSLMAIKSIPQSIGRAFAAAPGTPADRV
                     ATLRDALQKAVNDPELIAEAQKAKIDVSFISHEQVIKDFTALLNQTPETLKEMGKYIK
                     AES"
     gene            complement(47312..47935)
                     /locus_tag="VF182_02000"
     CDS             complement(47312..47935)
                     /locus_tag="VF182_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6435873.1"
                     /translation="MKRYDCLRAIAPQFGEELVVTNIGAVRHEWQALRPHPANYHLQN
                     LGLTSSMALGLALALPHRKVIAFDGDGSLLLNLGALATMANQHPKNLIHVVFDNECYE
                     SSRGAPTATAGQADLAAIARGAGFANVITARTMDEFEKGFLRALKSNDLYFILAKVEA
                     GAGDVPAAALDSQENKYLFVRYIEKSENLKILTSTLSRASDPAPKSK"
     gene            complement(47940..48440)
                     /locus_tag="VF182_02005"
     CDS             complement(47940..48440)
                     /locus_tag="VF182_02005"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6435874.1"
                     /translation="MKREVAAEIVDSLKEAGIDFVATLTEANLHDLVMALGEDTEIHH
                     VPVTREEEGIGICAGAYLGGKKPAMVMMNAGFLLSANALATVCIHPGIPVLMLIGHSG
                     GVGEENAGHATVGSLTEPVLQAMHILYEKVGCFDDIRPTIRDSQILARSSRRPTAIVL
                     NKQALR"
     gene            complement(48450..49442)
                     /locus_tag="VF182_02010"
     CDS             complement(48450..49442)
                     /locus_tag="VF182_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435875.1"
                     /translation="MHGRWLLGIVVLLTFHHQFALLVPVAAQELRRVRVAVTSPGMTN
                     LSLYIADRRGFFKEQQIFNEIIVMRSAARQIQALVAGSIELSAQAPDPLIRAVERGAD
                     LTMLSGLANSAAYDLVADKKIRSIEELRGTTLGVSGINSSSTLLLQKMLSAHGLSYPR
                     DYTLVEVGGTSDRLAAIKTGATSAGVLNPPISYVAVEQGFKILGELRDYIPAVQFTAL
                     SARRPWIYQNPKLTEDYLLALLRANRYVYENRSGTLTIIRDFFKVQSDHAERVYEYWV
                     RDKVIPADGAMTVPGTEVVLDILEQLGDFKGKIRPKAEKYIDVELLNRARKRMQ"
     gene            complement(49606..50220)
                     /locus_tag="VF182_02015"
     CDS             complement(49606..50220)
                     /locus_tag="VF182_02015"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX6435876.1"
                     /translation="MAMSLEDRIDSTHGMLIVVDMQNDFCHRQGAACKRGRDMDFVQN
                     MIPRLIRLVEQARAHNFPICFVRTSGNQWTNSPIWTEFKNPDLLACAEGSWGAEFHAG
                     LEPRPGEMIVTKHRYSAFIGTDLDMLLRARGVKSLLVTGVGTGMCVFHTLTIGFMLDY
                     YITLVEDCSATTYGPGAHNEAVALVRKHYGRIASSDEIIAHWRD"
     gene            complement(50230..51240)
                     /locus_tag="VF182_02020"
     CDS             complement(50230..51240)
                     /locus_tag="VF182_02020"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435877.1"
                     /translation="MTHVLFKSLAGVSTAGLILSCWLLSPAVAADRLRIGLSSFTPIN
                     AAVWIAEEKGLFKKYGIDPEVIVVGGASAGGVSSLIAGDIQFLTGGGGAVINAALNGA
                     DVVMVASIVNKGVQRVMARADLKRPEDLKGKRIGVTRLGASSHMVLLLMLRAWKMNST
                     DVQAIQVGSSPAMMAALEKGGIDAAVLTEPTFFIAEDLGYRVLADLADMDIYYLHSMI
                     DTTRSYLRSHHDLATRFIKAYVEGIAYFKKNRAESVEVMKKKLRTAPAQTKYLERSYA
                     LYASGYFENAPYPSLKGASSVLEFLAKDNPRARSADPKSVIDASIVKELDDSGFIKKL
                     YE"
     gene            complement(52083..52895)
                     /locus_tag="VF182_02025"
     CDS             complement(52083..52895)
                     /locus_tag="VF182_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6435878.1"
                     /translation="MSDNKLIIADINETEGIAWLTLNRPEKKNALSIVLLSELAGILR
                     SLANNDKIRCIVTSGAGGTYSSGRDLYDMRGQENRRRTRGIGGVAEIVDIMRKLPQVT
                     IAVVRGWCLGGGLAMINGHDIVIAADTAQFGMPEVIRGSYGATATPTLFHAGIPFKKA
                     FFISLTGRNLNGIEAERIGLISQVVPEKELDSYIDTLVKEMASRNGATLENAKIAAYM
                     QKDLSFEMALKADDLVSHRMRYYTNPLSDVEGYLHSQKGGGTVKYVKPEDKH"
     gene            complement(52992..53999)
                     /locus_tag="VF182_02030"
     CDS             complement(52992..53999)
                     /locus_tag="VF182_02030"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6435879.1"
                     /translation="MDFTVIDADAHHLDGPAYKNYLPERFRIRSGPYFPAFGWDIFLN
                     DTTGRKPATPQEYCKDLDVEKIGDAVAYPSNALSIGLVRELDLVIELAKAYNNWACDF
                     CKQSGNRVKYAAVIAPQVVPEAVKEIRRSVQELGAVAVMVPTYVQSGMDLGQPEFDPI
                     YAEAQELGVPIGFHATAQVSQGNWRFHKYLGVHMTSHPFEQMLTIVAIISNGVLDRFP
                     KLKVAFLEAGVGWLPYWMERFDEKYLRRKSEMEPLKMLPSEYVKNNRCYFTCEGEESA
                     LPLVIEQFGDQCMMYASDYPHWDMEWPHSVSQVVRRKDISDGTKQRILADNAKAFYRL
                     S"
     gene            complement(54128..55822)
                     /locus_tag="VF182_02035"
     CDS             complement(54128..55822)
                     /locus_tag="VF182_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6435880.1"
                     /translation="MFAMMMRNPAQWLFLLTSVTLAYLVLPPFFFILHTSLVTDRGLQ
                     AGSFTIQHFTNIIDSLGDVKTLLANSMIFSVGSAGIALVYGTVLAWLAERSNAPFRKL
                     AYVSAYVSFAIPGIIKVVGWIMLLGPKAGILNAPAVAWTGQPLFNIFSMSGMILVESF
                     LWIPIVFLLMSTPFRSMDPSLEEAATTAGSSGWQVFRRVTFPLALPSVLAVLILTFIR
                     SLEAFEIPALIGIPAGVEVLTTKIYLQIKGGLIPKYGEASAYSIILIVLVGLGLIPYY
                     RITSKTYKFTTISGKNFRPNRIELGKWRWLGGFLMLALPLLQFVPIAAIAWSSFLPFA
                     QVPSRKAFSMLTLNNYATAFADSSIIHSVLNSLTVSISSATVAVLITFFAAWLIVRAS
                     IKWRWLLDQMAMLPLVFPGIVMGIAILKMYLILPVPVYGTIWILVLAFIARYLPYGIR
                     FSHSALLSLHKELEEGATTSGASWFQMVRHVVVPLIMPALLAGWIYIFLITFKELSIA
                     LLLYSPGSQVVAVTIWELWDNGHVGELAAFSLIITIGTVIVGSIFLNIAHRYGLQD"
     gene            complement(55955..57001)
                     /locus_tag="VF182_02040"
     CDS             complement(55955..57001)
                     /locus_tag="VF182_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6435881.1"
                     /translation="MKILGVLLLVFMLFPATLRAQARKPATISELVTYNGADREQVLY
                     DGAKNEGKLTWYTSLAGDSYKAMVKAFESKYSGVKIEAYRVSGSDITTRMFEESKAKR
                     FIADTVETTEGNLMFLRDAFLLRPYHSPHFASYPEEAKEKGERGLYFWAIARESYIGF
                     AYNKNLLSKGAVPKNFEGLLHPDLKGRMGVSLSDPIYKVIGAMLKTKGQEFVRKLKVQ
                     EIALHTIIPPALLDLIASGEVLASPGIFRNHVLVAVAKGAPVEWVPLDLVPTNVGGAA
                     IAAQPPHPHAALLMADYLLGAEGQALLAKYQYGSATKDLGFKRWRPDHGISTEKYEKE
                     LERWEKLLKEVSRR"
     gene            complement(57325..58419)
                     /locus_tag="VF182_02045"
     CDS             complement(57325..58419)
                     /locus_tag="VF182_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435882.1"
                     /translation="MRTNNLRLFFRLIGCSFFIAAWTQAFAAEKAPYYQGKTLTIVIN
                     FAAGGPTDIEGRVFGKHLGRHIPGQPAVVIQNMGGGGGITGVNYIGEVAKPDGLTAGY
                     FTGSLFHQQTKDPGLRVDLGKFGFITGVHGVTVSYIRADTPPGIKKPADFVKAERFRA
                     AGLGASSSKDVRFRLSFDLLGLKYDYVTGYSSSSTARLAVQRNEAQYHDETLPAYRAT
                     VEPQMVKTGEVTPLYYTDLVSPDGEVIVSPDVPELLPFTHYYRQVFGKMPSGIKYEAL
                     KAANMSSTNMTRLIMLPPNSPAETIAILRQAIVSLSKDSDFNAEAMKAMRFQPRFEVG
                     EQGERLFQKVSQTPPEIVSFIRQYIDQVNK"
     gene            complement(58878..59999)
                     /locus_tag="VF182_02050"
     CDS             complement(58878..59999)
                     /locus_tag="VF182_02050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6435883.1"
                     /translation="MDLSLTEDQEMLKKVAADFVKAEVPAHQMTQWYKNKNTFQPQLI
                     KKAAEMGWLGMMLPEEHGGAGLSVMDCAVVFEELGRGPVPGPLFTSGILSAQMIFEAC
                     TDEQRKSLLPSLCKGEAIVVPAITDKAAYWGPEAVETRLTKLSDRYIMSGTKRFVFDA
                     EAATSFLCAARSEEGKVVFVLVDAKSPGITITPHVGFFISVAEVRFDRVIVSPSHLLG
                     SSGASWAILEAALEKTLPVLSAYQVGATQEVFDMTCEYTRTRVVFGQPIGRFQRVQDH
                     CVDISIHLDQARWATYEAIWRIDSGMPARAAVHESKAVASDGYYHGTNYAHMVWAGPG
                     TDYGHPMMAHSVLAHTLYQYLGTPGHHKRLMMDALYPRG"
     gene            complement(60126..61340)
                     /locus_tag="VF182_02055"
     CDS             complement(60126..61340)
                     /locus_tag="VF182_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6435884.1"
                     /translation="MDFNVSADPPHVVEFRKEVAAWMAEQFAGHEQLRWSASWSTRED
                     QDEYKFRRGLAEKLGAKGWLFPIYPKKYGGADLTIDHQMVLETELFKYGLNLSHVFYT
                     LARIVAPVLIRWGTEEQKSEFLEPITRAQICVWQVLTEPHGGSDIANCQTKAIRDGDV
                     YVVNGQKVMVGHHLPPDYLWTLVCTTPGGKRHENLAWLHVPANLPGITIQHMHMMMGI
                     KNSVFFDNVRVPAKYLIGGENNGWKVASTHMELEHGGDGRIAADPLIERVVEYCRNTH
                     IDGKPIMEDPRVRDLIAEMTIELNTLRRFNQLIYWRRFAKKPHPYGGPQQRYYQRMLR
                     LRNGERLQAIMGADALEQNLSVNEVVDFEYAVRTGPGQLHGGGTLDTDRLIFARRLGL
                     GRPTTEKAPETD"
     gene            complement(61507..62673)
                     /locus_tag="VF182_02060"
     CDS             complement(61507..62673)
                     /locus_tag="VF182_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX6435885.1"
                     /translation="MEGFVCIDVSGHVAGPYAGSLLGDLGCDVIKVELPGGGDTHRGR
                     NPKYEGYGPSFRALNRNKKSVTLDLGQKKGREILFKLLTTADIFLENFRPPTRTKLGL
                     DYQDLAKINPKLVHCSISGYGQSGPYRDKPGFDTIGQALSGMMSLVTDLDDPKVAGIS
                     FVDHCAGIFSVHGITAALLARVKTGIGQFIDVSLLQVATAFIESHVADYLNGGEAVSR
                     ENFPKGRIYSLLDCDKKPFMVHLSGHNQAWEGLLEAAELDSLIGDPRFASRKQRTDRH
                     EEIVKILRQQFAGKARDHWLRRLDANSIPNAPINTIQEVFENPQVKHLGIPRQITHPQ
                     MGVTQLVGSPINLSGTPARFYRPAPLLGEHTEEVLGQLGYDKTALKELRNDRVI"
     gene            complement(63111..64118)
                     /locus_tag="VF182_02065"
     CDS             complement(63111..64118)
                     /locus_tag="VF182_02065"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435886.1"
                     /translation="MVISTVFTSKRGIGVLSLILLAALVCGVAHAADRIRIGYSGVTV
                     SNAMLWVTDEGKLFHKNGIDPQILYLRTTLGQTAMIAGEVDMCVYSGSLLSSARLQGA
                     DVVMVTSFLDKPLYRLVVRPEIKTVADLKGKRLGITRFGTVTDSMTRLLVGKLGLDPD
                     KDVSYIQVGDVPILLASLTAGKTIDGAIIQPPYYLKALASGMRVLVNMQEMDIPVQQT
                     GLNTTDKFISKNPDLVRRVVKSLIEGIHLMRTDPTVAKRALSRRMQIKDEKEIDDTYL
                     LLKSFVPIKPYPTLDGFKTIFDDLSKRVPAAKTANPKEFVNTRFIEELDRSGYIDSLY
                     K"
     gene            complement(64090..65076)
                     /locus_tag="VF182_02070"
     CDS             complement(64090..65076)
                     /locus_tag="VF182_02070"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435887.1"
                     /translation="MITAKRRIVFPFIFVLLFLSDTSLAADRLRISYSGPSISNALLW
                     VSKEGKLFEKNGLDVEVIYLAGSLGQSALIAGETQFAVYTGLLMTPARLQGADVTMVV
                     SFLDHLLSRLVVRPDIRSVADLRGKRLGITRFGTASDFGMRLMVSKLGLNPDKDVSIL
                     QIGDNPTRLVALQAKSIDGAIFDPPEYRKAVEAGGRVMFNLEETIIPYQHAGLVTTGK
                     YISSRPDITRRVVKSIVEGAALLRKEPLIAKRALSARLHSKNESELAETYQLLNGFTR
                     PKPYPSLDGFRAILSSLMNRMPAAKTADPKDFSEPKFIEELDQSGYIDSLYK"
     gene            complement(65340..66392)
                     /locus_tag="VF182_02075"
     CDS             complement(65340..66392)
                     /locus_tag="VF182_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435888.1"
                     /translation="MKQQAFQSERIEIDDELWAANDFFEAKGWSDGLPIVPPTEDRVA
                     RMLSATKRKADEVIGTVPPKWASATVEKIAINAVMAGCKPEYMPVLIAAVEAITDPKL
                     NLYALQATTGGPAVMLIINGPVRHRIQVNGGYNALGEGWRANATIGRCIRLIQRNIGG
                     SYPETTCKATLGWPGKYSICMGENEEASPWEPLHVERGFDRNSSTVTAISADGSVRAS
                     DLDSTTAEGVLTNFAQKMDGPSGPEAIMVICPEHAKIIANDGFSKKDVQQFVWQRAAY
                     RMKDLPEETFNQRVKRWSHRNLTRESIIPVTDHPEDIIVVVAGGDGSQSQYIHVWGQS
                     TPEGGSTRSVTKAVGE"
     gene            complement(66703..66987)
                     /locus_tag="VF182_02080"
     CDS             complement(66703..66987)
                     /locus_tag="VF182_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435889.1"
                     /translation="MIVLSPEGKARTSSERIAALPRFADLNGKTVGLLDNSKPNNDML
                     QARFEQLLRQNCDVAGVVRRRKLTAQQGAPKAYLDDLAAHADFVISGLGD"
     gene            67418..68005
                     /locus_tag="VF182_02085"
     CDS             67418..68005
                     /locus_tag="VF182_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435890.1"
                     /translation="MQVLDTVSIEDLVALPPTIFGVIRWKKYIGRPQGDLCSGFNIRV
                     EEHTATQFRAVGGGKYEPIPGTGIWKDVVDAVSCRELADEGDYHKLSFQVWGVHLNAF
                     PDGRYRITPTLKGTWSRPANPVLFFGSRTIEPQSFSVVLKKDDQIQSVEFELVQRPRP
                     LGTILSAAVVGAIVGSALQSARPNRWLSARSGPGS"
     gene            68130..69668
                     /locus_tag="VF182_02090"
     CDS             68130..69668
                     /locus_tag="VF182_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6435891.1"
                     /translation="MLGSIRPALLTLVIVLTIAYAPAYAQSPAALLQQAADAMGGLSA
                     LGALKTQVIESEGKQFDASSTARPLGPTRQITTFRYTLTRDLTRPRLRLVWEGQSLGS
                     KQSSRFVEIIDGSIGMLQEGGNGAKQTRLHPGRLATRVREEHRNPAKLVLIGLNNKSL
                     RHRGDALLDGKRQAVLSFTENDEEFRVYVDRETRLPAQTEILEDDPLEGDSSYTLRYG
                     DWRKVDGVMLPFSLRYELNGNTLQEEQIKSIRHNAAIPADMFAVPDSVRREKTDAMPI
                     ASQWILRRVAGNVSYQDMGRPAVVEWAQLAPGVHKIQGSSHATILVEMRDYLVAVEGP
                     LYEARTAPVVKSIKERFPGKPIRYAIPTHHHLDHAGGIRAFMAEGAAIVVPFNARAFY
                     EKVARSAHTRNPDSLERNKRAVIIEAFGGGHRVLTDGERQIAVYPLPTSHAEDLVVVY
                     LPKEKLLIEADHISPRNGDVRPGPRVKEFVQQFDKLNLDVATIVGIHGDQASLEATRG
                     AAKK"
     gene            69772..70122
                     /locus_tag="VF182_02095"
     CDS             69772..70122
                     /locus_tag="VF182_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435892.1"
                     /translation="MPVDTSDDLITGGSFSHDTHDLLSIASIYMACVGLGLIFAPQRF
                     DVGAVPTDPSAALIAYLRLFGSPFLGIAVLNWMARSAGALHGAQCHHRWQHCRIRRYS
                     CVGSMGDWPAARGH"
     gene            complement(70359..71297)
                     /locus_tag="VF182_02100"
     CDS             complement(70359..71297)
                     /locus_tag="VF182_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX6435893.1"
                     /translation="MESSDLRVFEAVARLGSITAAADELHTVQSNVTARIRALEDQLG
                     TLLFRRHSRGVVLTRAGERLLPYAVQISQLLKDAVHVVGESGPPHGNLQIGSLESTAG
                     VRLPPILSAYRRKYPDVNLMLTIGTTGDLIEEVLACRLEGAFVAGPVKRDEIVETQVF
                     TEEMVLVTSAKFRSFYEALRSPQGVNILVLRAGCSYRSRLERLLKEAGVTSFGQLEFG
                     TIDGVFGCAREGLGITMMPRSVSEHASQNAELGIHPLPRRDALVPTMFIRRKDAVLSA
                     ALGRFVECALACATAAAARTRSKRKTKRPAKLFKIA"
     gene            71470..72549
                     /locus_tag="VF182_02105"
     CDS             71470..72549
                     /locus_tag="VF182_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF561 domain-containing protein"
                     /protein_id="HEX6435894.1"
                     /translation="MSIRTALTKRLGMTYPIFQAPLAGGGDTPALVAAVCEAGALGFI
                     GAAYLTPEQIGEAAHKVRTQTSRPFGINLFAPVPMPDPPENPRPSLERVAPFFAELGL
                     EPPVVPAPARDSFNQQLIAALESGAAVFSFTVGILPKGAMDAIKRRGMFLIGTATTVE
                     EAIVLEKAGVDAIVTQGSEAGGHRATFGADFDAGLVGTISLVPQVVDAVTVPVIASGG
                     IMDGRGIAAALALGASAVQLGTAFLTCDEAGVPEVYKKAILGAREDQTRLTRAFSGRP
                     ARGIVNRFMLEVESSASADAILPFPLQNQLTRPLRNAAAKQGRAEFLSLWAGQGVRLA
                     QRQAAALLVETLATETERVIQRLAN"
BASE COUNT        16903 a        19602 c        20190 g        16287 t
ORIGIN      
        1 ttttttcacc ggtttcaaag tcttcatgta ggcgatcagc gctttgagat cttcctgcgc
       61 cattcccgaa taggcctcat agggcattac agggaccatc ctgctgccgt cgcgccttct
      121 cccggcgttg atagcgtcgt agatttgccg ctcgctccaa tcgcccaggc ctgtttcctt
      181 gtcctgggtg atgtttgtgc tgaacaccgt gccaaatgga ataggaaagg cgcggccgcc
      241 ggtgtggtag gtttctttgg ggacagtatg acaggcgcaa cccccggcga tggcgaatag
      301 gtactggcct ttcgcgatca gctctttatc ggcttgggcg tagagtgtag ttgatgttag
      361 aaaaaaaccg acggttagga ataatagaac tcgcctcgtc ctccgccttc gtaatactcg
      421 tcgcatgttt taacgtaaag cctcctcgcg aagtttttta tccaagaaac tcaaggtagc
      481 atgaaacact ctgttgatcg gcgcctgctc tccggcctgc tcgagcaaac gcgtgacgat
      541 gccgttaagc gcgtcgcatt cgagcggctt tcctgcttcg agatcctgca gcatcgatgg
      601 cttaaaatca cccaagcccc tggagaactc cattgtttca tccaacagtt ccggcttcag
      661 gtcgatgccg cgggtgcgcg ccgcggcttg aacctctttc atgcagaggc gggcgagatt
      721 gctgagagct gccgattcga cgatctcacg aaccgtggcg cgggttaagc acgaaatagc
      781 gcagaacgcc gcattccaaa gcagcttcgc ccactgaact tgctcgattg cggcgctgat
      841 gacgcatggg atgccacccc gcgatatggc ttgctcgaca attagagcgg tgtccgtact
      901 gtgatcgttt atgggtccga gaacgatttt tccaccggac gaatgctgga tcacgccagg
      961 ggccgacatc tgagcgccaa tgtaaacgac gccggccaag actctctcgc tgccccagag
     1021 tttggctagc ttgtcgggat tatcgatgcc attctgcagc gaaagaatgg ccgttctctc
     1081 ggtcaacatc ggtgccagcg ccgctgccgc tccggccgtg tcgtaagact tgacacagaa
     1141 gagaaccagg tcaaccatac cggcgtcagc cggcgagtcg gtgaaaaacc catttcgcac
     1201 ctgtagatcg ccgcttgggc tttcgatgcg caatcctttc cggcgcagcg cgggcaggtg
     1261 tctttgtggg gcgatgaata cgacatcgtc gccggcagcc gcaagtttgg cgccgaagta
     1321 tccgcccacc gctcctgtgc ccataaccgc aactcgcatg tttgtccctg cgcgtgtccg
     1381 actgtattct cgcctttggt cgatgtcaat ttcacctcat ttcaaagtgc ctcgatttac
     1441 gctttgatcg tgaatgtgtt atgagaattc aactatggaa aagatcgatc cgcagcacga
     1501 tcatttgaaa cttgagcagt attgtcccgg gcagcaagtc atgtttagag gaaaaacgta
     1561 tactatccag cgccggacca cccttgcttc gggcgacgcg gcgatcgtgc tgcagggcga
     1621 taaagagcag ttcgtgattg gcgcagatcg gtttttcgcc ggagttaagt aacccggggc
     1681 acccgaagtc aatcgtttac cagtcaatag gagaacagca tgggaaaaat ccgcctgaca
     1741 ctggcctgtt gggattatga tcgcacccgc gcgctgcaag atggcagcgt cgaagtcgaa
     1801 ggcatagatt tgacttattt acccttgcgc gtcgaagaga ctttttggcg aatgctgcgt
     1861 tacggagaat tcgatgcggc ggaaatgtcg acaggctctt accttatgtc gcgcgacaaa
     1921 ggcgcgccta aattgattgc catcccggtc tttccgtcca agacatttcg ccattcatgt
     1981 atttatatca acaccgattc cggcatcagg aagcccgccg atatggcggg gaaaagagtc
     2041 ggggtccccg agtatcagat caccatggct acctgggcgc gaggcgtcct gcagcacgaa
     2101 tacggagttt ctccggaaaa gatgaaatgg ttcaccggcg gcgaagaaca tcccggtcga
     2161 gaggataagg tgaggcataa cttgccagcc aaaatcgata tccgaccgat tccggcggag
     2221 aagacgctct cgggcatgtt ggaagcaggc gagatcgatg ccatgatttc agcgcatatg
     2281 ccatcgccgt tcgtaaaacg tcatcccaaa gtgcagcggt tgatacccaa ctttcgtgag
     2341 gtcgagcaag actatttccg ccgcaccaag atctttccga tcatgcacac ggtggtgatt
     2401 cgcgaggaag tttacgagaa aaatccttgg atcgcgcaga gcctgttcaa ggccttcgat
     2461 gaatccaaga agctatgtca ggagagcatg tacgaattct cggcgctcaa atatatgctg
     2521 gcgtggtcca tcgatgagat ggaaaaagag cgcgaagtcc taggtgatga tccctgggcc
     2581 tacggcttgg aacccaatcg ccatgttctt gaaacccttg tgcagtacac ctacgagcag
     2641 ggtctgatca gcaagccttt ggatgtcgat tcgctttttg ccaaaagtac gctggaggaa
     2701 ttcaaaattt agcgggcgcg gtaaccacgg cgacttgtat ggcagacgaa aagaacgaaa
     2761 ccctcgaaga aagcaaatgg tggatcgacc gcggcccggt caaggagcgg gaaaagccgc
     2821 tcggccccgt gccgggaatt atcgagaaat gggcctacga gctgctcagg ggttacaatc
     2881 aggatcaggc ggcgaagaac ctcgtcgata tcaactcccg gctgcttaaa gaattaacgg
     2941 tggcccctgg ggaatggggc tacttctcga tggacgtcgg aacctatccg ccggagaaat
     3001 tggcaaaaat caagaatggc ttgccgttac ctcccggcgc ccgcgggctg atcgtggatg
     3061 ccggctattc agtacgggtt gtcatcaccg gcgagcccgt gggggagcgc tacctggaca
     3121 tcaattttac cgatgcgccg gcccttgatt attgggtgga tggacaattg gttcgagagc
     3181 gggttttctc aaacgtcggc gaggcgctca agagaattcg cgccgactta ccaaagtacc
     3241 tcaagaaaac tgaatagcta tcgtcataga tgcggcaggc tgggatacca cagacaattc
     3301 cttgttccga ttagtggact cgagccgtta atgagcttaa tgattcttgg ctttagttcg
     3361 tttttgcttg gcggcttccc gaagcgggtt atcacgcgga atcggcatca gcgatgtcac
     3421 ttcgagcccg tagccgcgta gtccgaccaa gtgcttcttg ttattggtaa ggagccgcat
     3481 cttacgcacg cctaaatcgc gcaagatctg cgcgccgatg ccatactctt tgaagtctgc
     3541 ccgatagatg aacgaaagat ttatgttctt ttgttgctta ccgtgaaccg tcggataagt
     3601 aaccatcgcc ggagttagcc ccttgtgctc aggttgcaaa taaagaatta cgcctttgcc
     3661 ttctctggcg atgatctcca ttgagctctg aatgaccctg ccggtgttta acagctcgaa
     3721 gccgaaaaca tcgcccggca cgtatttcgt gtgaactcgc actaaagtct cttctttggg
     3781 agatatattg cctttgatca aggcaaggtg ctcgctttga tcgacgtggg tattatatac
     3841 aatggcctga aactgcccgc ccatacgcgt cggcagcggc gcgctcgccg cctgataaac
     3901 cagagaatca taattaagtc gatattggat caggtccgca atcgttacta ccttcagctt
     3961 atgcttgcgc gcgaacctca tcaaatccgg ttggcgggcc atggtaccgt cgtctttcat
     4021 gatttcgcag atgactccgg cgggttttag gccggctaag cgcgccaagt caaccgagcc
     4081 ctcggtctga ccagctcgta ccagaacgcc accgttgcgc gcgcgcaacg gaaaaatatg
     4141 tccgggcgtc accagatcct gcggcttggc gtcgtcatca atcgaatcga ggatagtctt
     4201 ggcacggtcc gctgccgata ttccggtggt gataccgagc acggagtcga tcgagaccgt
     4261 gaaggcggtg ccgaacggag aggtattttc cgataccatc attgaaaggt tcaacaatct
     4321 caccctttcc tcagttaggg tgagacaaat aagtccccgg ccgtgagttg ccatgaaatt
     4381 tattgcctgg ggagtgactt tttccgccgc catgcacaag tcaccttcat tctctcgatc
     4441 tttgtcatcc atcaggatga ccattttccc acggcgaata tcttcgatcg cttcttctat
     4501 agtagccaga ggcatagcgg caataactct agcatctcag cttgtgccgt caaatagtcg
     4561 tggctctacc caagcggtct ggaatagtgc cgttaactac ttgtcgccag aaacagctta
     4621 agggcctgtt gactttcgat cgatcaatgt tagcgtaggg ttggcaaaat accttgagat
     4681 ccacctattt gattaagaaa acccttatgg tgacggcgat cctgatactt ttcacgtgcg
     4741 gggtttttgc cataagccta gcatacgatc cggcttcgga acggccatcg tacaccaccg
     4801 tctcgcccaa tcacgggtct ggcgacggtt taatcggaac cgatacatct gctctgagac
     4861 tcgcagttat ctgtctgagc ggtgatgttc cgcgctcggc acagccaaat tttcaaactt
     4921 acaggattaa ttacgtccga gttgtcgatc cagttaagtt gttctttttc ttagagaacc
     4981 acgtgcccaa ggtttccctt cagattctcc agtcagtgct gttgatttga cttttctcgt
     5041 ttaactgttg tcttgctcga tttagccccg actccgacgg gctatccgtg gcgtcgttat
     5101 tgtgctttat ccgtctgctg tcagacgcag gcgtgatcgc cacgaaacac tggttttatt
     5161 ctaaaatgga ggttttcccg tgattgcgaa aaaacgattc gaggcacaaa tttcgaataa
     5221 tgtttcgaat aatcatagcc aaggtcgatc gcctttttgg gtcaagagtc ccggacaatc
     5281 caatgccgcc tctgaagaaa aggcgtttgc tgtatttcaa tctgacattc tcattcctga
     5341 gcagtttctt gccacttatc agaggacctt tcgacttgac cctgagaagg tgctcatgct
     5401 ggcagttttg caggacgctg ttacgtgctt tcaggagaat attacagcaa cgtgcaagcg
     5461 taaacgggcg ctttaccaag aggcggagga atggtttatt gcagaggaca aatcatatct
     5521 tttctctttt gagaacatct gcgaggccct gggctttgat gtcagttact tgcggcaggg
     5581 attgctgcgc tggaaggaca gtgcgggaga gacacaaagt agccaaccgg taactcgcgc
     5641 ggcgggctag caaacatcag gattaagcga ttgtcagatc gctgatcaga tactgaagct
     5701 ggacagagcg gccgattgac gcaagaaaat ccccgctctg cccagcgcat caggaccgcc
     5761 cggatagtgt cattggcaat tcatcgggct tactcttttc cgacaaactg ctagcgcgca
     5821 tctaaccgtc catcattcga gggatcaggc aatatgaaag taggattgat cgggccgcgc
     5881 ggcgccggta agacgaccat tttcaatatg ctgaccgggt taaatgctca ggtcggcggt
     5941 tttgccggca aggaagaagt tcatttgggc gtcatcaaag ttcctgatcc ccgcatcgac
     6001 aagctctcgc agattttcaa acctagaaaa acaacgtatg ccgagattcg tttcactgac
     6061 tttccaccaa gtcaggggga agataatcta aagtccaaca gtgcaatggt ggcacagatg
     6121 cgcgaggtcg acgccatcgc tttggtcctg ggcaattatg agcccgatgc caaacctctt
     6181 agggaactta atcatctgct cacggaaatg attctggccg atctcgctat catagaaaat
     6241 cgccgctccc gtcttaagaa agaaaaagcc cggccgcttg aagagagcct gttgcagcgc
     6301 gcggcggctg ctctggaaag cgaagaaagc ctgcgaaact tgacgttcac tcccgatgaa
     6361 gagagtttgc ttgcgggttt cgggttttta agccgtaagc ctctgcttgt gctgctgaac
     6421 cagtcggaag accaagctgg gcaagagctg gattctgctt atgtagaaga gttgaaacgg
     6481 cgcggcttgc agggcctggc gctggcggcc aaggccgaga tggagatcgc tcaattggac
     6541 gaagccgatc gaattgcgtt ccttaaagag attggcatag aagaaccggc gcgagaccgg
     6601 tttattcgcg cctcttatcg cctgctcgat ctgattagtt tctttacctc cggagaggac
     6661 gaagtccgag cctggaccat tactcaagga accgtggcgc gcaaagccgc tggcaagatc
     6721 cacagcgata tcgagcgcgg cttcattcgc gctgaagttg tcgcctatga agacttcatc
     6781 gcgtttggta gcgagtccaa atgcaaggag gccggcaagc tgcgtctcga aggcaaagac
     6841 tatgtcgtca aggatggtga catcgttcac ttccgctttg ccgtttaatc agtagttgaa
     6901 tcgctattgg gccactcatt tacaaatcgt tctcggaaat ctcaggatca gaaacaacgc
     6961 cactgataac tgcaaaaacg cagaccgtag caaattctcc actaccatca ggagctcacc
     7021 acctaaccct ccgggccaaa gaacctagca acgattctca tcacagctca gactctagct
     7081 tgtcttagcc accttctttt gttagtgctg ccggtgtccg aaggataacg aaaggttaac
     7141 catgaaaact ttagttcaag gcggattcgt ggtcgggttt aatgggacac aacacgagat
     7201 catcaaggac ggtatcgttg tctatgatgg cagtcgaatc gaatttgtcg gcaaagacta
     7261 ctctcaagct gtagacagaa agatcgacgc cagaggctgc ctggtttcac cgggcttcat
     7321 cgacactcat ttccattcgg ggatcaatgc cagcgattac attttgaacg attcaaccaa
     7381 gctggatttt tttgccgcca attatctagc tcacggcggg cctacccgtg acggcgctcc
     7441 ttccgctcac ttaaaggatg tcgatgttgg ccaacgtttt tcgttgatcc atctattaaa
     7501 aagcggagtg acgactactt tcgagatcgg cggtccgggc ggagagccgg agcggttcgt
     7561 cgacatggtc gacgaggtcg gcatccgaac ggcgacaggc ccgagctaca agaacgtcag
     7621 tttttttcac gaccgcgagg gccgcttgga atacgactgg gacgaaactc gcggcagcga
     7681 agggttaaaa aaagcggtac agttcgcaaa gcaatttgac ggggcttgca acgggcgcat
     7741 gaccgctatg ttgtttccgg ggcacgtcga ctcctgcaca cctggtctgc tcaaacaaac
     7801 caagaaagcg gcaagcgatg ccggtgtcgg aatccagatt catgcgacga tcaatctatt
     7861 tgaattccat acggtgatgc aacgggaacg ctgcacgcct atcgagttgc tgcaaaagat
     7921 cggcttcctg gatccgcagg tttctctttc ccattgtatt ttcatcagcg gccatagctg
     7981 gcttgcctat ccgtatggca atgatctcaa gattattgcc gattccggcg cttcggtcgc
     8041 ttacagcccg ctcaaatacc tgaagctagg tatcaccatg gagtccttcg accggtatct
     8101 taatgccggg atcaatatgg gaattggaac cgacactttc cccaaggata ttctctcgga
     8161 tatgcgttac gctgcgctcg cgtcgcgcgt cgcggataag agttttatgt cgggccaccc
     8221 gcgagacgtc ttcaatgcgg cgacactagg cggggccaag atgctcggcc gcaacgatct
     8281 gggaagactg gaaaaagggg cgaaagccga tattgccatc gtcaatctgc gcgatctcac
     8341 atttggagcg gtgcgcgatc cgattcgatc gttgatggaa aatgccgtca gccgtgatgt
     8401 gagaaccgtc atcgtcgatg gcgaaacact ggtggataat tacaagtacc ttcgcctgaa
     8461 tgaggaggag ctgctcgaca gagttcaggc aaaaggggaa caagtctggg acagtgttcc
     8521 caaatggcat tggacgggaa agagtattga tgaagtagtg ccgccgagct ttaaaatgaa
     8581 atagccgtta cgaacccagc taggcgacct agccagaaca gatcatcact tgtacgcggc
     8641 aggagctctg gtcgccgaag accagcagcg catattgccc gctagacatc gataatcgtc
     8701 gcagtttagc ggtcaaacct caattctatt tcaaacccgt tccacccaga atcgcgtgcg
     8761 cgctcagcct cagtcagctt cggttgaagt atcaattgag gaaagatata ggacagacaa
     8821 cagtccaact gaaggagtat atcgtgtccg aattatcaga gctcaaaaaa aagctggcga
     8881 tgtcatcacg catgttgttt aacgccggat tagtcgatta cagcggtcac atcagcgcgc
     8941 gtattcccgg ttcggatcac cttgtcattc tgccacaccc ggtcagtcgc gctaccgtga
     9001 aaccggacga tatggtgatc acggacttcg atggcaaatt gatcgaaggc aactacaaag
     9061 cgccctcgga ggttaacatg cacgcgcgcg cctataaagc ccgtaacgat atccaaagcg
     9121 tcgctcatct gcacaatcat atggttgcca ccctaagcat ggtcgacaaa ccgttctatc
     9181 cggcatcttc taatcccggc gcattttttg gcccggggcg cttgcctgtt tacatggatc
     9241 cagcgctgat tcatacgatc gagcagggtg acgcagtggc aaaaacgctg gggacggcgg
     9301 acgctgtcat gctgcgcgga catggctcga tggtagtcgc ccactctatc gaatgggtat
     9361 ttgccggatg tgtggatttg gaagaagccg catcgcggtt ctataaagct tcgttgctgg
     9421 gacccgtcat gatttacaac gaggacgaga cccaaagggt catgaaacag cggcgcaaag
     9481 actcggtggt tcagaaaatc tgggatcaca atgctgctaa gacagagctc gccggcctga
     9541 tgaacgggat ctaaacacca gtaagatcgc cggtttcgaa gcgcatcgtg ggattgtgat
     9601 agggcggagc ccgaaggccg gatttatctt tctatgctcc agatgatgtc gtccttcccc
     9661 gcccttcgag gtgaatggga gctcaactgg gcagtaaatc tgctcttaac tttactggca
     9721 gcgctgcgtt atagtccttg gcgactttat gatgatattt gttgcgcgga agaaactcgc
     9781 gtgatatagc tggcgattca atttcatcca ggagtgtttt cgcatatgta cgatgatttt
     9841 cttgcaagaa tccttgagcc tgatgttttt ctgccggctc aattctatgg caccggaggc
     9901 ctgtcacgtc agctcgacgg tgagaaacgt ttgatgattg ctattttgaa ggacgccgtc
     9961 gagtgtctca ataagtaccg cggcgcgcga agtagtttag gaagcactca gtatcagaat
    10021 gccttggaat gggtccagga tgaaggcacc gactggctgt tttcgttcaa caacatttgc
    10081 gatcttctgg ggtttgatcc cgactatctt cggaaggtgc tgatcaaaaa agaagctaga
    10141 cacgcaaatc ccgtccgtgg gaagatctat tcttttccca tcggcagcgg agccggtgga
    10201 ggctcaccgg cggcgcgctc gtaaattatt cagctcttct tttgcagtct gcccgcgtgc
    10261 agagttttcg ccgaggatct tgattgcctt ttcaaaagcg gccatggccc gctcgtcttc
    10321 gtcctgtagc aacaaggacc gagccctata gtaataggcg tctccaagcc gatctaggtt
    10381 cccgtagaca atgccaagcc gggcaggcgg cttatcccat aatggtgaga gctgctgagc
    10441 gttcaggtat gcgcctgccg ctccccgtaa gtcattttgt ttttcaagta gctctccgag
    10501 aaacaaataa ttaagcggtt ccttgggctc catggcgatg gcacgatcaa gtgtagtacg
    10561 ggccgcgccg aaatcgcccg acgcggtgta taggcggccg agatcgcgac acaaaccggg
    10621 gtcttccgga ttgagtgcca gcgccttctc gtaattcgtc ttcgctagtg aaagctggcc
    10681 ctttttatgc tgcgccagcg ctagaagatg aagcgccata acttgtgacg gagactgcgc
    10741 gaccaatttt tgatattcgt cgatcacctt gtcagtctct tgccgctcta accgaataag
    10801 aacctgtatc tttttaagct gatcgagact ttcggattta ctctgaacat cgccgagtga
    10861 gcgaatcacc aattgaacgt tagcaatccg ttcctgggtt actggatgcg tcagcatgta
    10921 ggccggcacg tctatcggat ttaaagatcg ttcctgatct aaggtcttga gaaagccaag
    10981 cgaacccgca gggtcatagc cggccgctgt catatagcgc acacccaaag tgtcggcttc
    11041 catctccaat tgccggctat atgcgatttg acgagtagca gaaacggctt ggccaacgac
    11101 gccggcagct tgtgcgccgg agccgctccg ggccaacaga agcattccca ccaaactcaa
    11161 aagtgaaatc gcatcggggc cggagtggcg cgccatgtga cgccctttga cgtgaacaat
    11221 ttcatgtccc atgactccgg cgatttcaga cgtgcttttc gcccgttcga taaggccgga
    11281 gtaaaagaaa accgaacccc caggcaccgc aaacgcgttt agctgggggt cggcgataac
    11341 aaagaaccga tactcgaaag gttgcgggcc cattacggag agaatccgtt ggccgatgct
    11401 ttcgatatac cgttcaatct caggattgtt gatgtacgta aaatgtttct ttgcctcacg
    11461 ccgaaattca cggctgatcc gtgtctcctc atcttcactg ggaggaccca cactcggaag
    11521 cggtgagcga taagaagcgc agcctgtaac gaagaggcaa attaccaaca accaggacca
    11581 cagctgctgg catcgacggt acctgcaggt ttgaatcact cgaagagtat agtgaggcgg
    11641 cggagggggt tcaacacgtc gtctcggcaa tgtgagcagt tgacgctgcc ggatggagcc
    11701 catattgtga cacgaccacc acctcgaggt attaatggcg cataaattcg aagttccggc
    11761 cgggaagcta tcctggcatt gcgatctttc ctatctgccc tttacgtgca cggccgagat
    11821 gacgccgttg gaggatttta tcggacagga tcgggcaatt cgcgcgatac aattcggcct
    11881 cggggtcaac aaaccgggct tcaatatttt tgtgaccggg cttactggaa ccggcaaaac
    11941 cagtatcgtc aaagcctttc tcaaaaaagc aatggcagag cgcgctcccg tcgacgccaa
    12001 tgccgaatcg cctgcggact ggtgctatgt ctataacttt accgatgcgg atcgtccgca
    12061 gattatcaag atgcgccggg gctggggtag ggtcctgaag gccgacatgg aacagctgat
    12121 tcaatatttg caacgggaag gcagaagggt gtttgaaggg gacgaattcg cgcgcaatcg
    12181 ccagtcacta ttacaagagt tgcagcaaaa gcaacaaggc atcatggagc cgttgatggt
    12241 cgaggccgag aaaagcggct tcgcactgcg aatgactccg tccggggtcg cgctgctgcc
    12301 gatcaaagaa ggcaaaccga tgcaagaggc cgattacctc gcgttgccgc ctgaacagaa
    12361 gaaggccctc gaggacaagc gcggtgaaat cggcaagaga gtcgaagaga ctttgcgcga
    12421 aggaaagaaa gtagaacgcg agatcaacca aaggcttgaa gaactggaaa aaaaagctgg
    12481 cgaatatcta gtggcgatgc cgatagctga gatgagagaa aagtatcgtg ataatctgaa
    12541 actgttacgg tatttcgacg gcgtgcgcga ccatatctta agcagtttgc atcgcttcaa
    12601 ggcagccgag cctgattcgg ccgcagcgca gttgctgcct ctccagatgg cgcaaccggc
    12661 aacggacccg ttccttcctt atcgggtgaa tgtcatcgtg gataacagcg ccattcaagg
    12721 tccgccgatc attgtcgaga ccaatcccag ttatcacaat cttttcggtg tagttgaaaa
    12781 gaagcccgtg gtgggcggtt acgtgactga tttcacgctc atcaaagccg gttctatgag
    12841 caaagccaac ggcggctaca tcgttcttta tgatcgtgat gtcctggcca acacgggtgt
    12901 ctgggaagcg ctgctgcggg ttatcaaaaa tcgtgagctg cggatcgagg agccggcagc
    12961 cttttttggt tgggcgccgc cccagggagt aaggccggag ccgatcccta ccgataccaa
    13021 agtcattatg atcggcgatc ctcacctgta ccgcgctctg gccagcactg atccggattt
    13081 ccgtgagacc tttaaagtca aggcggattt tgactttcaa gttgaccgtt cgcaacaaaa
    13141 catcatcgcg ttcgcttgtt ttatcagcga ctactgtagc cgcgaaggct tgcgccattt
    13201 cgatagcagc ggggcggccc gagtgatcga gcattgctcc cgaatggtcg aagatcagga
    13261 aaagctctct actcgtttta gcgatgtcgt cgacctgcta atcgaatcgg actattgggc
    13321 gggaagagaa aaagccgaac tcgtatcagc caagcacgtc gagcaagcca tcgttgagaa
    13381 gaccttccgt ttgaacctgc tggagaagcg cctgcaggaa ttgattactg acgggacgat
    13441 cctggtagag gttcagggct ccgagatcgg acaaatcaat ggcttggcgg tgtaccagat
    13501 gggcgatttc agttttggca agccgtctcg catcacggcg aaaacttaca tggggcgagg
    13561 cggcctcatc aatatcgaac gcgaagccaa actcagcggc aaaagccacg ataaaggagt
    13621 tctgatcctg agcggctatc tcggcggcaa gtatgctcag gaaaagccct tatcgctttc
    13681 ggcaagcatc tgtttcgaac agtcgtatga cggcgttgat ggcgacagcg cttcctccgc
    13741 cgagctctat gcgattctct cgagcctttc gggggtgccg ttgaaacaga atattgcggt
    13801 taccggctca gttaatcaga acggggaaat tcaagcgatt ggcggcatta accgtaagat
    13861 cgaaggtttt tacgatgtct gtcgggtaaa aggcctgact ggggaccaag gcgtgctcat
    13921 gccggaatcg aacttacgca acttgatgtt gcgcgccgac gttgtcaaag cagcggaaga
    13981 cgaaaagttc cacatctatg cggtgcaatc gatcgacgaa ggcattgaag tgctcaccgg
    14041 tgttgccgcc ggggcacgca ataccgcagg taagtttcca gcgggatcga tcaacgcgct
    14101 ggttgaagaa aggctcacgc acttcgccga acggcagcgg cagatcaacg cagccggagt
    14161 tcgacacgat ccttcgctaa cgaccgaaac ctaagcgagc ctcgaacgtt gtttggcgca
    14221 tgggttcgca ggcaggaatt ctgagttgcg atcacttcgg gtaacactaa aaagtttaag
    14281 ggccgcagcc gaagagattg gtgaacgcgg gtttccgtgt ttgccctgga gaattgatgc
    14341 agaagaaggc agccgggaac agccaatgag cattagcctt cgtaatggag gaaaaaatgg
    14401 attttgactt aaccgatgag ttagttgcgg tacgcgatct ggcgcgggag ttcgctgaaa
    14461 aagaagtggc tccgacagcc gccaaggacg atcgagagca tgcttttagg ggcgacctgg
    14521 taaagaagat gggtgagctc ggattttacg gcagcgtgat tccggaaaaa tatggcggca
    14581 acggcatggg ttttctcgcg atggtgctga taacagaaga aattgctcgc gtccatagcg
    14641 ccatgagagt tgccatcaat atgcaaatcg gtccagcgct cgcgttgctg cagtttggta
    14701 ccgaagagca gaagcaacaa tggattccgc cgctagtgca aggggagagc atcggttgct
    14761 ttgccatcac cgaggctgac gccggatcgg atgtggcggc gatgcgcacg gcggcgacac
    14821 gagacggcga tcactatgtc atcaacggca ccaagctctg gatttcgaac ggaccggttg
    14881 ccgatggcgg cttgctctac gcctataccg atcgttcgca aaaacatcgc ggcatgaccg
    14941 cgttttatgc cgatctgcgc ctgccgggcg tgactcgcaa gcccctcgag accatgggcg
    15001 cccattgttc acccctcggg gagcttacct ttgaaaattt ccgcttgcct ctaggtaatc
    15061 gtctcggcgc tgaaggagac ggctttaaga tctgcatgtg gcagttaaat cagacccggc
    15121 tcaactgcgc cgccggcgcg ttgggtctcg cccgcgccgc caaagaagcc gccatcaact
    15181 atgcgaaaga gcgcacccag ttcggccaga agatcggcga gtttcaaatg aaccaggacc
    15241 tgatcgcgca gatggtggtg catgaagaag cggcgcggct gctcgtgtat cgcgcggcct
    15301 ggcttgccga ccaaaaaaag ccgaacaatc tcgaaacttc gttcggaaag tacaccgccg
    15361 cggaagctgc ggcatttgcc gccgatacag ctatgaaaat tctcggcgct tacggctatt
    15421 ccacggaatt tcctgtcgag cgttactatc gagatgccaa gtcgtatcaa atcgtcgaag
    15481 gctcctccaa cgtgcagaaa atgatcatcg ctcaagacgc gctgggatat cggcaggcaa
    15541 atcgcggagc ttcatgatta cgcgatcaat cgttggatca gacccatatc gatactgtcg
    15601 cgcacatgcg cggcgagccg atcgtattgt tcatcaatcg agaacgagcc aataatcggc
    15661 gagaaatcct tgcctttctt ctcggcaagg ttgcgcaaaa gggcccaacg aaaatcatcg
    15721 ttgtgaaaaa gcccgtgaat gtatgtgccc agcacattgc catcggggct catgcagcct
    15781 tcgagatccg cgcatggttg agacccgcgt tgagtcactt cgaaagggtt aagcatgctt
    15841 gatcccgtcg ttatccccat atgaatctcg tatccccgca ccgtaaaatc ttttgctttg
    15901 gcaaacagac ctcgatccac cagcacacga gcactgatct gccgtgtcgc tttcaccgtt
    15961 agaaagcgac tggtcaccgg caagagggat aaacccgtga tcgttggtac cgccgattcg
    16021 atgccatcga catcatggat cgctagcccc agcatttgat agccgccgca aattcctatt
    16081 atcggtgtgc ctcctgcgac ctgccggaga atctctcgat cccgtccgtt ttccttcaaa
    16141 taagctaaat cgctgactgt ggttttggtg cctggcaata tgatcaagtt cggatcgcca
    16201 agatcatccg cggcttcgac gtaacgcagg ccaacgccgg cttcccgttc caatggatcg
    16261 aagtcgtcga aattagcgat gtgaggaagc gcgatcacgg cgacgtcgat gtcgactgcg
    16321 cgttgttgtt tcatcgctcg gcgcttttct acaggcatgg aatcttcctc tggaatgtaa
    16381 atatcgtggt agtagggaat aacgcctgca accggcacgc cggttcgatt ctctaaccag
    16441 tccaacccag gctttagcag tgagatgtcg ccgcgaaatt tgttgatgat gaatgctttg
    16501 atatgttctt tctcggattc ttcgagcaat tgcatggttc ctattaggga ggcgaaaacg
    16561 ccaccgcggt cgatgtccgc ggcgagaagc acgggagagt cgcaatacag cgccacttcc
    16621 atattgacca gatcccgatc ccggagattg atttcagccg ggcttccggc tccttcaatg
    16681 ataacaacgt cgaagcgctc gcgcaggcga tcaagcgatt tgctgatggc cgtccacaaa
    16741 aaagacttga gctggggaaa atccgcagcg gaaactctcg tcagcggttt gcctccaacg
    16801 atgatctgcg agcaattgtc agcttcgggt ttgagcagaa ccggattcat gtcgatgctt
    16861 gccggaatcc cagaggcctc ggcttgcact gcctgtgcac ggccgatctc tccgccgtcc
    16921 acggtagcaa acgaattcag ggacatgttt tgcgctttga acggcgcgac ctttagtcct
    16981 cgttgtttga aaatccggca cagcgcggtg accagcacgc tctttccaac agacgatccg
    17041 gtcccttgaa ccatcaacgt tttgcctgtc atatgagtac cactgttctg ctttctagtt
    17101 aggtttgttc gactgtagag cggaacggat cgcgtggaat gaagaagcgt aaaccggttt
    17161 cggcatcttc ggcggccttc atgtctacgc ctaagaattc cttgatgttt gtctcagtga
    17221 tgacttcggc agcggctccg caggcgcaaa gctgtcctga gccaagcagc agcgccttat
    17281 ccgcgaatag ggaaatcaga ttgacatcat gtaaaacggt caggacggtg gtctgctgct
    17341 cgacgcactg tctcttcagg gcggacagaa tctgcagctg atggtaaaga tccaggtgaa
    17401 ccgtcggctc atccagcaga aggatgccgg cagactgcgc caacgctcgc gcgatcaaga
    17461 ccttctgctg ctccccaccg ctcaatgtcg tgaaggttcg ctcactgaga tgaagcgagt
    17521 gggttgcctc tagtgctctt ctcactgcta aattgtccac gctcgagaat ctgaagcggg
    17581 aggcgtgggg gaagcgaccc agcgatacca gttcaaatgc ggtgagcgga aaactgagcc
    17641 cggtctgctg cgaaacatag gcaattgttt ttgccagctg ttttggcgca tagacctgca
    17701 atctgcggct gtcgagcaat acctcaccgg cactgggtcg cagatgccca agaaggattc
    17761 gaagcagggt ggtttttccg gaaccgttcg ggccgatcag cgccgccagc tcaccggcgc
    17821 cgattgaaaa cgaaatgttt gttaaaacgg gctcgttcgc gtagctaaaa gaaagctcct
    17881 gtgcctgaat ggcgactctg cgattcattt agaaggtgta acccgcgggt tctttgccga
    17941 tgaggtaaag aaagataggc acgccgagca atgaagtaac gatgcccact ggcagttcag
    18001 cgggagctat gatgatccgc gcagcggtgt cgcacaggac tagaaagaca cccccggaaa
    18061 aaaaggttag cgggaacaaa gttctatgat cggcgccgaa gatgaggcgg atcatatgag
    18121 gcacgatcag gccgacgaag ccgatcaatc cggcgacgga aaccgaaatc gaagttaaca
    18181 gcgccgcaag gatagcgtag agcaactgaa tccggtcagt gcgaattcca ctgctgctgg
    18241 ccgtctcctc acctaaaagc aaaacgttga gctcggaggc gaaatagcag gcgaagatca
    18301 gcgccagcgg cactaatcct agtgaaaccc aggttgtatc ccaactcgaa ctggaaaagc
    18361 cgccaagaaa gaagaagatc agcgatttca gctcttcacc ggcgacgagc atgaagatgg
    18421 ccaccagtga agaaagaaag agaccgacac ctagacccga gagaagcagt cggaagatcg
    18481 agaagccgcc gttaaggtgg cccaagtaaa acaccaatcc caacgccatg agcgaaccga
    18541 gaaatgcaaa aaaactgata gctgcgaaac tggctactgg agaagcgagt cccaacacca
    18601 cggcggctgc ggctcccagg gacgcgccgg atgagacgcc gagcagataa ctgtcggcca
    18661 gtggatttcg aaacaggccc tgaaagatgg ccccggctag cgccagtcct ccgccaacga
    18721 aaaaagctga aaggctccgg ggcagacgca agttcaacaa aattgaacgg tccgttgcag
    18781 acatttcaaa aaaagaaatg ggcatcggtc caacactaat tccgagcacg aacgagagaa
    18841 atacggcaat gccgatcagc aaccagctcg gtttcttcag cttcaatttg cctccggcgc
    18901 cggccgtgac gcgtttctag tgtcactaac caggcagccg tgaatcttct tgagcccgtt
    18961 tacaaattga acactgcgtc tgatgtcccg ctcctcgata aaacagaacc gctcgttccg
    19021 gactccgtca atgacgctcc agcccgcttg tgttttaatt cgttcggcga acggtttctg
    19081 atcaccgagg aatatcagca cttcgggatt gcgctggata atcgattcaa ggctaatttg
    19141 ccccgagtcg aagggcatgt cgccaaaaat gttaatgcct ccagcggcgg caaccagcca
    19201 gtgaggaaaa gaggaccgtc ccaccgtgat caacggctcg gtccacatct gcaggtacac
    19261 ccgttgtctc cgatgattcc tggcgctggc ctcgatttcg agaagttttc tttgcgtctc
    19321 atcgagtatt tgctccgcct tgtgctctcg tccgagcagt tgcccgagga gcaagaagtc
    19381 cttgcgcaga ctggtcatat catgaggcca gagactcaac accgggtaac ccatattctt
    19441 cagtggcaca gcaaatctct gctggctcgg gccattcgtc agaatcaaat ccggctggag
    19501 tcgaacgatt ttttcgagat tggcgtcgaa tcctccgccg acaatatcga tcttcttgtg
    19561 atcaacccgg cttttcagct cttggggact ttcgtcgtaa cgggtgccgc ctttcagtct
    19621 atcgcaaacc agcaggcata ccatctcgga attcgtcggc actaaagaca cgatccgttg
    19681 gacattttgt ttgatcgcca ctttgaagcc gagatcgtca attacgatcc gctctgccgc
    19741 aaccgcagaa gaccctttta acaatagcat tgtaaacagc gcgaccgcga tggttgcggt
    19801 tctcaagccg acattgtgaa gcggcgggcg agttcgcatt tcagtaacgg aacgaaagac
    19861 cggcaaagac attgatgcca ggcgcgggaa cacggaaaga ttcgttgaac ggcgctccga
    19921 ccaagattcc tgaggtggag tattttcggt cggtaagatt gttcaaagtc acgtgcgcca
    19981 gccattcgcg ccattcgtag gcgatgcggc tgttcagcac aaaataatcg gcgagttttt
    20041 ttgccgcatt cggttcgtct cgcaattggt actgctcgcc cacataattg ccgaacaagg
    20101 agaacgttag ccgatctaaa ggacggacat tcacacccag gccgacccga tgacgcggca
    20161 ccagtggtag ctcgtcacct ttcgtgaccg gacggggggg atcgacagcg ttgatgccag
    20221 gaatgaagaa atctgtttca aaggttgcct tggtcagcgt gtaattgatg aacgcatcga
    20281 tccatttatc cggacgcagc ttgaacgaaa cttccacgcc ttggcgcagg gttctactga
    20341 tattttcatt acggccggaa aatgggttta gggggtcggt ggcgataaat aggatttcgt
    20401 cccgcaccgg cgtgtaaaac agggatatcg cggcttccag ccattcagct ggttttgcct
    20461 tgaatcccgt ctcaaaattg cggcttctca tcggctccag gttcaggttt gaaccgaacg
    20521 gccccaacgc gaagatttcc tggacagtgg gtactcgaac tccctgtgag taattaaaat
    20581 aaaggcttaa attctcaacc ggagtatagg tcaaaccggt cttagggcta aatcgtttga
    20641 attccttggt tccgctcaga gtcggatcgg tcttaccggt gaaattcaga cggtcccagt
    20701 catagcggaa cccagcgttg aggatcagag aatcgaaaat gttcaagcta ttggtaaaaa
    20761 aaactccgac aacgttctct cgggtcgatt gctcggtaat gaagtcgcct ggacagcaga
    20821 taaacgcgga agcgctggta gtttcaaaac gattgtggcc gtactcgagc ccggttgcgg
    20881 caacattctt tttcgccagg atagttcctt catggctgat ttgaatggtg ccgcctgccg
    20941 agagggtgtc agtgtctaga tttgaattgc ttgaaagccc gcgtacgaag atttgctgat
    21001 catggttgcg caaaaatcca ttgacagcca gcgagaatgc cgctggcagc ttttgacgga
    21061 cgttcaatcc gatcagatcg agattggaat ccgcaaaatc cccgggggtc aggttgtgcc
    21121 gacgccgacc cgctcggagg aagctgcctg gcagtgaccc cgcttgcttt agatgatcct
    21181 ggacgtgagt gtaggagagg gtcgcatcgg tcgagtcgct gaactggtaa ccggctttgc
    21241 caaatagccg gctaattctg gcgccggtgc cgtcgcgaaa accgtcgctg agctcgcggc
    21301 taacattaag cagataatcc aaattcttga gcgccagcgg cccgtcgcta ctcaatgaat
    21361 atctttgtcg agcgtagctt ccggcgccga ggtcgaaacc gatatgcggg cggtctcgac
    21421 ggccgcgctt cgtggtaatg ttgatgaccc cgcccaacgc attgcgtccg aaaaccgtcg
    21481 ctgtgcccgg aagaatttct aaacgctcga tactctcgat tggaatcaaa tcaaagttga
    21541 tgctgttgaa atccggctca ttgacgcgaa cgccgtcgac aaacacactg gtaccgggga
    21601 ctggctggcc attgaaaccg cgtagatcga cgcttgcctg aaactcgttg ccgatggcat
    21661 cgtaaagaac tattcctgtt tgatgctgaa gcacttcctg aaccgtactt gcgcccaact
    21721 cctcgatctc ttctgtggtg atgactatta ctttgccggg tatctgggca accggctggt
    21781 cgatgtcccg tagtcgcgtt gcagtgacta aaaccggcgg aagagttttg tcagttggtt
    21841 cggcagccgg gagtgcggct gcgaaaagta ctaatacaac aaaacaaaat agcgctttca
    21901 tgaaaacctc cttttcccgc gaaaggggat tgttgcggca gcacggcagg tctcctggct
    21961 tgagcgtcaa acctacttgc cgcgccttcc catccgcgac tttggatttt agatctccga
    22021 ttttggattg aaccaaagtc aaaaacctaa attcatgaat cgattcggac agtggctcgc
    22081 gatgcggcgt tcgtagctct tacagttgcg gggcagcaga ggactttccc ctcgccgcga
    22141 caccaccatt ttggattttg gatcgccgat tttggattca atctcaaatc caaaatctaa
    22201 aatcgaaaat cgctggtcgg gtatcgtggg aggaagagtc acctctttcc ctatagccgg
    22261 ctgcggttaa tggatgtttt tactggctcc ttcctaattc aaatgggatc cagtcttctt
    22321 gcgcgcgcga acggagaaag gcccggctgt ttcgaattaa aaacagaagg tggctccagt
    22381 tcggggcgga gactagttcg ggattaacaa tacacaacct ttctctctct gtcggagagt
    22441 tcggtgtaag gctctaggag gtctcctggc ttacgggttg gagaggcttt cctcgcctat
    22501 ctttcacgcc ttcccatcct tgtgcgatcg cacaggacag tggctgccgg tgaaagatct
    22561 tacccgatta cagtggcggg accgcgccgg attcgcaccg gcttccgttg atcttagagc
    22621 caaacttatg tcgcggaaat tacctgctga gctggagatt gtcaattcga aattgcgaat
    22681 tgaatccgct gagaggctgg ccatcgacca ccgagactgc aatcatcaca ggattaacca
    22741 aaacacggtt gggatcgtaa ccaccgaaat aaaagtgctc agcacaacga tggacgcagc
    22801 gagctccggg tccttgccat acttttccgt gagcacgaaa ttaatcactg ctgccgggag
    22861 agcgccgtaa agcagcaaca cctggcgatt gaccccttca gcagcgatca attgaacgac
    22921 gatagatgct gtcagaaatc cgccgcagat tcgaacgaga gcgccgccta cggcatggcg
    22981 ccatttaaac gactggaatt catggaggcg gtaccccaag ctaacaagca tgatcggaat
    23041 ggttgcctgc ccaagcatgg ctattggttg aaagagcagg tccggtatgg tcattcggtt
    23101 aagattcaac attaaaccgg caaaggttgc atagatcaaa ggcagacgaa aaatttcgat
    23161 ccaattgcca cgcccgctca gaaggtaaat gcccaatgaa tactgaagca acgtaatgat
    23221 cacaaacatc aacgtggcgc gttgcatgcc cgcttggccg aaggcgaaca atgccagtgg
    23281 aatgcccata ttgcccgcat tcatgaacaa cacaggtaga gcaaagccgc gcgagctaaa
    23341 acggaacaat gagaagtaga gccggatcag taatcctaca ccagcgaaga tcagaagaac
    23401 gcccgctgct aggacaaaaa tatcgtttaa caagattggt ttactggcca aagaggtaaa
    23461 cgccagtgaa ggcgttccca aatagacgat gagttccgtg attggaaata gagtgatttt
    23521 cttccagtgt gcgaagcaaa agccgatggt gattagcaca aaaaccggaa caacagggaa
    23581 gaggggaatg aagacttcca tctcggatag gtctgacaat tagagtgttg ccaacagcac
    23641 caggctcaag gcttcgctcg cttcgacgac ggcgccgaag ttatccttag ttagcgcgcc
    23701 ggttcgcttg tggaacagag ctcgacaaaa cagggcgaga attgaaagac aaagcccgat
    23761 ccacagggcg gtccggccaa ggaagtaagt tgccggcgcg agtgtcgcaa tggttgcaaa
    23821 cacaaaatgc cagaatctca tgttggccgc gataatgccg gcttcccctt cactgtgtcg
    23881 atatgaaccg tagataaaaa ccagcatcgt ccagcgcgcc attacaggga ttagtaataa
    23941 agcgacagtg agcctctctt ctaggatatc cagcgacttg attttgaaaa gtatcacgaa
    24001 gacaatcgcg ataatcccaa ccgcgctgct agatgagttg ctccccgccg ctggttgtct
    24061 caacgctggc gcatcgaacg ttttttttaa tccttctaag tgaacaccgc cggtcaccag
    24121 taccagaaaa gcgactatcg tcgtgctgag aagttctgca tccaaatagt tttgaagtgc
    24181 gcgcgcgagc agcaccaggc tcacgccaat cactaaaccg accacaggga agagcaccga
    24241 tgctgtacca accacggccg gggttggttg tgagtgtata agcctgctta aaatcgtaag
    24301 aaattttaac gcagcgacaa tgcttttcat ccgcttcctt ttctcttaga gatgtggcgc
    24361 tgtcaatcga tgggcaattt cgcacgtccc attggagttt ccctaaaatc gctcgcaagt
    24421 taaaatatta gtcaattaaa tcaagacctt aaggggcggt tttagcttta cttttgcgcc
    24481 cgattcaggt actatgttgt tagtctcaaa tcgggtctaa ccccagaact ttccaggcaa
    24541 tatttcagtt gatatcaggt gaaaatggct caatcaaaac cacctcagca aaacgactat
    24601 aaagccgaca atataaaagt ccttgaaggc cttgaagcgg tgagaaaacg ccctggaatg
    24661 tatatcgggg acaccagtga acgcggcttg catcacttgg tttatgaggt ggtcgacaat
    24721 tctatcgacg aagctttggc cggccactgc gataacatcg aagttaccat tcatattgat
    24781 aatagtgtga ctgtggttga caacggtcgc ggcattcccg tcgacattca ttcgaccgag
    24841 ggagtttcgg ctgccgaagt cgttctaacc aaactccatg caggcggaaa gttcgataaa
    24901 tcctcatata aggtttcggg aggtcttcat ggcgtcggca tttcagttgt caatgcactg
    24961 tccgaaagtc tcgaaattga gatcaaacgc gacggcagag tttatcaaca gagctatcga
    25021 cgcggcgtgc cgcaggaccg tcttaaagag gtcgggagat cgaaggaacg tggcaccaag
    25081 gttacgttta cgccggatac cgagatcttt gaaaccgttg agtttagctt cgacattctt
    25141 tcccagaggc tgcgcgagct cgcattctta aatagcggcg taaagattac tatcgaagac
    25201 gagcgcagcc aaaagaagca cgagtttctc tataaaggcg ggcttctctc tttcgttgag
    25261 catctcaatc gcgccaaaac cgctattcat ccaaaggttg tccacttcgc cggcgaaagg
    25321 gatggggttg aggtcgaaat cgccatgcag tggaacgatg gctattcaga aaatgtcttt
    25381 tcctttgcca ataacatcaa caccgccgag ggtggaaccc atctgatcgg ctttaagtcg
    25441 gcattgacac gcactgtaaa cagctacgcc atcgacatcg ggcagctgaa aaaagacgat
    25501 gcaaatctgc agggcgaaga cgttcgcgag ggtcttaccg cggtggtgag cgtcaaagtc
    25561 cctgagccgc agttcgaagg gcaaactaaa acaaagttgg gcaatagcga ggtcaagggg
    25621 atcgtcgagg ccctcgtaaa cgagaagttc tccagctacc tcagcgagca tcccgcggac
    25681 gccaagaaga taatttccaa gggtgttgag gcagcccggg tgcgggaggc gacccgcaag
    25741 gccaaagatc tggcgcgacg caagggcgcg ctggattccg ggtcattgcc cggaaagctc
    25801 gccgattgtc aggagagaga tccggcattg agcgagattt atctcgtgga gggagattcc
    25861 gccggcggtt ccgcgaagca gggacgagac cggcgcaacc aagcgattct gcctctcaaa
    25921 ggaaagattc tcaacgtcga gaaagcgcgc ttcgataaga tgctgtcatc gcaggaaatt
    25981 cggctgatga tcaccgcgct tgggaccggc gttaaagaag attatgactc ctctaagctg
    26041 cgctaccact cgatcatcat catgaccgat gcggatgtcg acggctcgca cattcgcact
    26101 ttgctgctga cgtttttcta tcgccagatg cccgatttgg tcgaaaaggg acatttgttc
    26161 atcgctcaac caccgcttta caaaataaag cgcggcaaac aggaacgtta tctcaaagat
    26221 gaagcgaccc tggaagatca tttgatcgag ctcggcaccg agaatattcg cgttcgtgcc
    26281 accaatgacg gcaacgggtt agccggtcag tcgctgcaga gctttgtcaa gaaagtcgcc
    26341 cgttgggaaa agctgatggc tctgattacg cgaaaaagaa aaaatcgggc ggttgtcgaa
    26401 ggtctcctcc tcgaagagca gttcagcgaa gacacattaa aagacgagaa agcgctgcgc
    26461 gctttggaaa gccggctggt atcttacata aaccttacgg ctccggatca agcgccggta
    26521 tcgtgcgccc tggaagaaga cccggaacat aattgcttca agctgatctg cgccactcga
    26581 acaaacggtt caaggtctca aactattatc gatcgcgagt ttctgattag cgctgagtat
    26641 aaagaaatca aaaggctgtt cagcgagttg acgagtatcg gcgtgccgcc ttacgcggtg
    26701 gaaaacggcg aacagaaggc ccagcttcgc tctcttactg aggttctaag gtacattatg
    26761 gagggcggca aaaaggggca gtatatccag cgctataaag gcctgggcga gatgaatccg
    26821 gatcaactct gggaaaccac gatgaatccg gaaacacgga tcctgttaca ggttagagtc
    26881 gaggatgcgg tagaagccaa cgacatcttt tcgactttga tgggcgatga agtcgagcct
    26941 cgacgcaaat tcatcgagga tcacgctctc acagtaaaaa acctcgacgt ctagttcaaa
    27001 accagatatt cgggcgcggc cagacgatct ttagaacaaa cggcagcgcg cagctctcta
    27061 cttcccgact aacttatgga agcgacattt aaatcgcaaa aagtacctgt ctatatcgaa
    27121 gacgagatgc ggcagtccta catggactac gctatgagcg tcattgtcgg ccgggcattg
    27181 cccgacgtcc gtgacggact caagccggtt catcggcgag tgctctacgc tatgtacgac
    27241 atgggcaata cctgggacaa gccgtataag aaatctgccc ggctcgtcgg agatatcatc
    27301 ggcaagtatc atccgcatgg cgacacggcg gtctacgaca cgatcgtccg gatggcgcag
    27361 gactttgtca tgcgttaccc gtttatagat ggccaaggta atttcgggtc ggtggacggc
    27421 gactccccgg cggcgatgcg ctacaccgaa atccgaatgg ctcgtctggc agaagagatt
    27481 ctcgcggata ttgaaaaaga caccgtcgat ttcattccca actatgatga ttctctgaaa
    27541 gaaccggcgg tgctgccgtc gcgcattcct aatcttctgg tcaatggcgc ctccgggatc
    27601 gcggttggaa tggcgactaa tattccgcct cacaatatcg gcgaaatcgt cgatggattg
    27661 gttgcaatgg tggagaatcc tgggatcacc atcaaagagt tgatgaagta tattcccggg
    27721 ccggattttc caaccgcggg cttcatccac ggcaaagaac cgatcgttca agcttacagc
    27781 gacggcaaag gcatcgtgca gatgcgtggc aaagcgttca ccgaaacgct caaacgcacc
    27841 ggaaaagaac agattatcat cagcgaaatc cctttcatgg tcaacaaagt gcggctcatt
    27901 gagcagattg ccgccttggt ccaggagaaa aaaatcgacg gtatctccga tttgcgcgat
    27961 gagtcggacc gtgagggcat gcgcatcgtc attgagctga agcgcgacgc cgtgcccgag
    28021 gttattatca atcagctgta taagcatact tcgctgcaag agtccttcgg gatcaatatg
    28081 ctggcgatcg tcgacggaag gccaaagctt ctcaacctcc gcgatgtcct ccgggcattt
    28141 ttggatcacc gtaaagaggt cgtcacccgg cgcaccgctt tcgatcttcg aaaagccgaa
    28201 gaacgcctgc atattttaga aggtctgagg attgcgctgg acaacctcga tgcggtgatc
    28261 acgctcatcc gtaacgctaa cgatccgaag gcggcgaaag acggcttggt ggcgagcttc
    28321 gggctatcgg aacttcaggc gcaagcgatt ctcgacatgc gcttgcagag attgaccggc
    28381 cttgagcgcg ataagattct ccaggagcat aaggaaacgg tggaactcat cgccaagctg
    28441 ctggcgattt tggccgatga aaaggagatt tataaaatca ttgtcaccga gctcaaagat
    28501 atcaaagaaa aattcggcga cgagcggcga acgcagatcg ttgcccgctc ggaagagatt
    28561 tccatcgagg atatgatcgt cgatgaagag atggccgtaa ccatctcgca cgagggttac
    28621 atcaagcgca acccggtgac tttataccgg gcgcagcggc gcggcggcaa aggaaagatc
    28681 ggcacgacga ccaaagagga agatttcgtc gagtacttaa tcatcgcctc gatgcactcc
    28741 tatattctgt tttttacgac caccggcaag gtctactggg tcaaagtcca cgaactgcca
    28801 caggccagcc gggctgcaaa gggaaagccc atcgttaact tgcttaatct ggaaccgggt
    28861 gaaagggtct cggcttttct ttcggttcgt gaattcgtcg agggccggtt cgttgttttc
    28921 gcgacgaaga agggcgccgt caaaaaaacc gatctaatgt tgtattccaa tccgcgagcg
    28981 tccggaatac gggccatcgg cctggaagac ggcgacgagg ttatcggagt gcggcttact
    29041 gatgggcagc aagagcttat tctgtcgacc ttggatggcc agtccatccg cttcaaagaa
    29101 gatcaggtcc gtccgactgg gcgcggaact tatggcgtct tgggtatgcg tcttgattcg
    29161 ggcgacgccg tggtcagtat ggagatgctc aatcgcggcg ccagcatttt gactgtctcg
    29221 gaaaagggtt atggcaagcg cacggagatg gaggagtatc gtctccaatc ccgcggcggc
    29281 aagggtatca tcacgatgaa ggccactgaa aagactggcc gcatcgtcgg cggacaacaa
    29341 gttaccgacg acgatcagct catgcttgtg accaacaatg gtaaaatcat tcgcctgcgc
    29401 atgagggata tccgggttat cggacgcaat acacaagggg tgcgcctgat cgatgtcgag
    29461 gagggcgagc gagtggtctc gctggcgcgg ctagccgaaa aagaggaaga cgaggaagag
    29521 ccggaataga tttttcccgg accggccggt tcatctgaac gcctgatcga tcgagtcccc
    29581 cctttaaaac tgcccgactt atcaccaaat tatgggaaag ctgattagca ttatcggcgc
    29641 tggcggttgg ggcaccgcgt tggccaccgt cttggggcag aagcgggccc gcgtaaactt
    29701 gtggtgtcat ggcgcacaga cctatcgtga gctaaaggaa acgaggcaga accgcagtta
    29761 cttatccgag attatgatcc cggatacggt gaacgtgacc cgctcattgg ttgaggcagt
    29821 gacggatgcg gaactcgtct gctgcgtcgt gccgtcgcac gcggtacggc cggtcatcag
    29881 cgaggccgcc tctcatatca attcgaaagc ggtgatcgtc tgcggcacca aaggattgga
    29941 agaagaaact tctaaaacga tgggcgaggt tttactcgaa atcttcggag agggcgccgc
    30001 acaaagacta gcattcatct ccgggccgac ctttgcaatc gaggtcgctc gaggattgcc
    30061 cgcggcgctt accgtagccg cgtattccga aaacatcgcc gccgaaatcc aagcacttct
    30121 gagcacctcg agcctacggg cctatacctc gaaagacgtg attggcgtgc aaatgggcgg
    30181 agtgatcaaa aatgtaatcg caattgctgc gggtatcagt gacggcttgg gattagggca
    30241 taatgcacgc gcggcattga ttactcgagg cctagctgaa atgacgcgat tggccattag
    30301 aatgggagcc gagccgttga ccctggctgg tttgccggga gtcggcgatc tgctcctcac
    30361 ctgcaccggt gggttgagcc gaaatcgtca ggtcgggttg caaatcggcg ccggcaagag
    30421 ccttgaagaa ataagccacg ggtcgcggat ggtggcggaa ggcgttcgca atgctcgatc
    30481 ggtctatttc ttggcccaga agctgggcgt tgaaatgccg attgtcgagc aaatgtaccg
    30541 aatccttcat gaaggcaaga agcctgcaga gggcgttcag caactaatgc agcgttccct
    30601 taaggctgaa cgggcctaaa ttgacaatgc caagactgta tgattcaaat agcttagccg
    30661 cgaaaagtcc actctggttg gtagtgcccg atcgcgtttc gctcatgttt ttcgttcaat
    30721 ctgccgtgaa ttgtttttta tgccgagcgg atttttctga tgcggatgga accaactgtg
    30781 gccattcttc taatgtcctg atttagtcaa cggagcaaaa atcgtcatga agagaactta
    30841 tcaaccaagc aatatcaaac gaaaacggac acacggattt ttggctcgga tggagactcc
    30901 cggtggccgt aacgtgctca gacgccgtcg cgccaaggga agaaaaaaac tgacggtaac
    30961 cgtgccgccg aaacgagcca gaacctagac atgccgattg cggtcggagc gggttttcct
    31021 aaacaagccc gcctcaggaa acgaccggaa ttcatccatc tctcgcgctc cggtaaaaag
    31081 caacacaccg ccaacttcgt aatcattacg agagagaccg atcgatccga agcccgcttg
    31141 ggaattaccg ttagttcgaa ggtgggcaat gcggtggttc gcaatcgaat gaagcgattg
    31201 ctacgggagt gctttcgaca gtggaatcat caaatcgtgc cggcccggga tatactgatc
    31261 atagctaaac cgggagcggc caagctctcg ttcttacagg tggcgagcga aatcagtcgg
    31321 tacgttaccg atagccggat ttgcaagcgc tcatgatagg attttttccc caggtcatga
    31381 tcggtatctt gggtgtctat caccgattca tttcgccatt cttgccgcaa gcatgtcgct
    31441 tcttcccaag ttgttctgtt tacgtcgcac aatcgattta caaatatgga ttgataaagg
    31501 gagcatcgct tggcgtgaaa cggcttagcc gttgtcatcc ctggcacccg ggcggctacg
    31561 atccggttca ttaaataatc ttataatgga aaaacgcgct tttttagcga tagctctttc
    31621 tttattggtt ctggtgcttt atcaggaatg gattagccgg caatacccgg cccctcctcc
    31681 gcaatctccg gcagagcaga agacagaagc cggaaaggct caaactgctg attcggttac
    31741 tccactaccg cccgtcgaga tcaagccggt gcccacaccg gcgggaaaac cgatcgaaga
    31801 cattcgcatc gaaaccgaca actacattgc tctgtttacc acccatggcg ggcgcctgaa
    31861 aagttttaaa ttcaagcact atcacgctac ttcagatccc aacagcccgc cgttcgagat
    31921 gattgcgacg gtaccgggag tagcttatcc cttaggagtt aagtggagca gcgcgtcgcc
    31981 cgcggatgat gcatcggtta tctatggagt tgatggcggc gaccttaaac tcagcggcga
    32041 agccaaagga acgcttacct tccagggaac cgcgccggat ggagcgaccg tcaccaagaa
    32101 gctgactttt tccggagcgc agtacccgat cgaactggag attagcgcca gatctgcaaa
    32161 tggcagccct ctggcgcccg cgactttgct cactgctccc gatcagcatg ggaaacccaa
    32221 tcccgaggcg ccgttcgagg ggcttctcac cgttgtcgat aataagatca agcgcgaaca
    32281 cgtcaccgat gagctgcgga aaggtaaaaa tttttccggc cagctttcct gggctggatt
    32341 cggatttacc tatttcatga tggcgctggt gccggagaat ggcgcaggct accgagtcga
    32401 cgtgcaacag tccgggcccg ctctgatact cacagtcgct gagccaaccg gcaagcccaa
    32461 ccctagtcag tataccttgt ttgtcggccc gaaagagttg agcattttgg agtccattgg
    32521 caaggggatg gaaaaggcca tcgactttgg ctacttcgga ttcatctcaa ttccgttcct
    32581 atatgtcctg catttttttc atcagtttac ccgcagttat ggaatcgaca tcattcttct
    32641 aacggtgctg ctcaagttat taactgcgcc gctgacgcac aagagctatg cttccatgaa
    32701 gcagatgcaa aagcttcagc cacaaatgga acggttgaaa gaaaaatata aggacgatcg
    32761 ggaaaagctg aacaaggaga tcatggaatt gtaccggcgc aacggggtca accctctcgg
    32821 aggttgcttg ccgatggtcg ttcagtttcc agtttttatc ggtctctaca acgcgctcta
    32881 catacccatc gagctgcgcc atgcgccgtt tctgtggatc aacgatcttt ccaagcccga
    32941 ctggcaatcg ctgccattcg agtttgccgg ttggcatctg ggcgttcccg tgcttacgat
    33001 actgatgggg gcgagcatgt tcatgcaaca gtggatgacc ccatcagcgg gagacccgaa
    33061 tcagcgaaag ctgatgctga tgatgccgct gatatttacc tttatgttcg taacttttcc
    33121 ggcggggcta actatctact ggctagtcaa caacgttctt tcgatcgccc aacaatggtg
    33181 gattaatcgc agcgtgaagt aggccgttag cgaggtacat tgtatggatt acgtggaaac
    33241 cgaaggggac agcatcgacc aggcaataga aaacgccctg accagtctcg gggttgagcg
    33301 tgagagaatc agcatcgaga tattgtcgga aggaaaacgc ggtattcttg gttttggcac
    33361 ccaaaaggcg cgggttcggg cgtcgctaag aagaccggcc gttgaaacga accacctcga
    33421 gccagcagcc gagcctgacg ttgtcgccag tatatcgccc gagcccttaa gtgacgcgga
    33481 aacagccgcc attggcgaaa aggccaaagt tgttctgagt gagatcttga aacgaatggg
    33541 gatcgaggcg gcggtgcaga caaaatccgg ggacaaagcg gacgagatcg ttctggaagt
    33601 gatctctgat gatagcggcc tgctgatcgg tcgcaaaggt caaactctgg aagctttgca
    33661 gtacctagtg acacgcatcg ccggtgaccg gcaaggaagc gaagggccgc acatcctcgt
    33721 cgatatcgaa aattaccggc aacgccggaa aaaatcgctc gaagacatgg cgttgcgcct
    33781 cggtgaaaaa gccaaacgac aacgaaaaac ggtaacggtg gatgctctaa gcgcggctga
    33841 tcggcgcatt gttcatgccg cgctgcagga cgatccatgg attacgacca aaagtcttgg
    33901 ccaagggtcc tatcgacggc tgctcataat tccggagggg gatcgtaagc ggaaagaaga
    33961 gttgaagtcg gtcgacaaac aaggttcgag atctcagagc aacattgaca ggcccggccc
    34021 tgcccgaacc tcctgaaacg ttcgctgact cacgcttgcc tctgttcgcc accgcaacgt
    34081 tcaatctttg attgaccgct cggtgtcagt ttcctatagt gaacccgtga tcaaacgtcg
    34141 cgcaacaaga cagattcagc ttggtaaggt caaggtgggc ggagacgcgc cgattaccgt
    34201 gcagtcaatg accaagacgg acacgtccga tgtccgcgcc accgtgcacc aaatatggtc
    34261 cctggaggcg gcgggttgcg atatcgtccg cgttgccgtg ccagtgcgcg cggcagcgga
    34321 acagctcgcc gagattaaaa agaaaatccg cattcctcta gtggccgata ttcacttcaa
    34381 ctataaacta gcgctgattg ccttggagca gggtgtcgat ggtttacgcc tcaaccccgg
    34441 caatattggc gcgcgccgct atgtcgagga ggtggtcaag ctggccgccg agcgcaagac
    34501 tccgattcgt atcggggtca atgccgggtc tttggaaaaa gatttactag ataaagccgg
    34561 cggtccaacc gtcgagggca tggtcgagag cgggttgcgc catatccgca tattagagga
    34621 tttgaatttc tcccagatca agatttcact taaagcgtca gatccgctga tgatgatcga
    34681 agcctaccga atgctcgccg ataaagtgga ttatccgttt catttgggtg tgactgaagc
    34741 gggtacgccg acggttggca ccatcaaatc cgcggtgggc ttaggaactc tactcgctga
    34801 gggcattggt gatacgatac gcgtttccct ggcggccgat cccgtcgaag aagtgcgggt
    34861 cggcgccgag attctaaaat ccttgggtct gaaaaaagaa ggtttgacct tcgtcgcttg
    34921 tccttcctgc ggtcgagcgg atgtcgatct cgtgggcctc gccaaagcag ttgaagaacg
    34981 gatgattcct tacaacaacc tgaatatcca cgtcgcggtc atgggctgcg aggtcaatgg
    35041 cccgggtgaa gctcgtgctg cagatctggg ggttgccggc ggcaaaggca tcggcctgct
    35101 gttcaagaag ggtcaggtaa tccgcaaggt gcccgaagct cgcattgtcg atgccctcat
    35161 ggaagaagtg cagaagttcg ccgaagagaa aaaggctgag cagtctgccc cggccgacga
    35221 ttagccggtt cgtccaacag tctcaccttt ggtcccaagg attatatgcg ctggagtcaa
    35281 atactcattc caaccctcaa ggaagatccg tccgacgcgg aagttatcag ccacaagctg
    35341 ctcgtgcgcg gcggctttat ccgccaagtc agccgcggca tctacgacta tttcccgctc
    35401 gctttaaaag tcattcgcaa gatagaaaac atcgtcagag aagaaatgga tcgcgccggc
    35461 gcgcaggagc tattgatgcc gatcgcttcg cccgccgaac tctggcaaga aagcggccgc
    35521 tgggacgttt acggcaagga actgattcgc ttcaaagatc gcaacgaccg agacttttgc
    35581 ttgggcccga cccacgagga aattgttacc gacctggtgc gccgcgccgt gcgatcgtat
    35641 cgggagttgc cgtttaatct ttatcaaatc caaaccaaat ttcgcgatga agtgcggccg
    35701 cgcttcggcc ttatgcgcgg acgagagttc atcatgaagg acgcctatag ctttcacacc
    35761 gacattgacg actgcaagcg cgaatacgag aacatggttg tggcctaccg gcgcattttc
    35821 gatcgctgcg ggctaaagta ccggcctgtg gaagccgaca ccggcgctat cggcggcagc
    35881 ctgtcccatg aattccaagt cctcgcagaa tcaggcgaag acgccattgt gagctgtaat
    35941 aattgcgact acgcagcgaa ccttgaaaaa gccgcagtca ggccccagcg agcggcagcg
    36001 cgcgatctta acatagaggc gcctggtctt cagaaggtct cgactccggg aaaaaagagc
    36061 gttgccgagg tcgctgaatt cctcaagctt gcgcccgaga agtttatcaa gacaatgatc
    36121 tatcaagtca acggtgatga actggtagcg gctctggtgc gcggcgacca tgagatcaac
    36181 caggtcaaac tacaagcggc gctgggctgc cgtgaaatcg cgcttgccag tgaaacagcc
    36241 gttgtcgcgg caaccggtgt caccccgggc tatctaggac ccatcggctt gaaactgcga
    36301 accattgccg atctttccgt tcagtcgatg cgcggcgcgg tgacgggagc caacgaaccc
    36361 gacgctcact atatcgaggt cgatcaggag cgtgatttta ccccgacgac ttttgccgat
    36421 atcagactgg cgctagccgg agaccattgc ccccgctgcg accaaggccg gctcgaagcc
    36481 caccgcggca tcgaagtggg acaggtgttt tatctcggaa aaaagtatag cgaattgatg
    36541 ggcgctactt atttagatgc cgaaggccgc gagcgtccta tcgaaatggg ttgctatggt
    36601 atcggcatca gccgcttggt cgccgccgct gtcgagcaga atcatgatga caatggcatt
    36661 atctggccgt tatcgattgc accgttccaa gttttactac tgccgatcaa ctatacggac
    36721 aatgcgacga gagaagcggc cgacgtgttg tatggggaat tgcgccagcg cgggctcgaa
    36781 gtgcttctcg acgaccggga tgaacggccc ggagtcaaat tcaaagacgg tgatctaatc
    36841 ggaatccctt tgaggattac catcggcgcc aaaggattgc agaaaggcag tctcgaattg
    36901 cgctggcgtc gtgaaggcaa atcgatagac ataccggtta ctggagccgc acaaacgatt
    36961 cacagccatc tcagcaaatc gttccagcaa acgacatgac ctccaaactg gaactgctcc
    37021 gcgcacggcg gccgacgcgc gagcgtctga ttgtcgctct cgatgtcgac gatctcaatc
    37081 aggccaagga cttggtgcaa gcgcttgcca gtgaagtggg catgttcaaa gtcggcaagc
    37141 agctgttcac tcatgctggg ccgcaagcgg tgcgcctgat tcaagagctt ggcggcgagg
    37201 tttttctcga tcttaaattt cacgatattc ccaatacggt agccaaagcg gccgtggagg
    37261 ctactcgcat gggcgtcaaa atgttcaacg ttcatgcttc cggcagtctc gaaatgatgc
    37321 gcatgaccgt caaagaagta cggcgcgtat gccggcagga aaagcgacgc aggccgatca
    37381 tgctggccgt gacggtgttg accagcctca atcaagacga tctcaaacgg gtaggcatcg
    37441 acgggcgtgt ggctgctcag gtggtgcgct tggcgttgtt aaccaacgaa gcggggatgg
    37501 acggcgtggt tgcgtctccg cacgaggtcc gagctattcg ggattgctgc ggccggcgtt
    37561 ttatcatcgt tactcccggc atccgtccgg cggacagtag ccgaaacgat cagcagcgca
    37621 tcatgacgcc tgcggctgcc gtcaaagcca gcgtcgacta tattgtcgtc ggacgtccta
    37681 ttctcgaggc caaagatcca gttgccgccg ctcgctcgat cgtcaccgag atgaaatcct
    37741 ccagtaagga acctgtcggc gtcagctgat tggaatctcg tcagcgattc acgtcgcttg
    37801 ctggatttgc gcccgacatt attttcccca ataacgaaag aagtgatgca ctggaccatg
    37861 gccgcttccg accggaaagc ctttgcggat ggctgcggtg atgtaggttt tggcatgcat
    37921 gatagcgctc tccagcttct cacctttggc tagataggcc gcaatagcgg ctgacagtgt
    37981 gcaaccggtg ccgtgggtat gaatcgttcg gatacgcgga gtccgaaact ggcggaattt
    38041 tttgccatcg aagaataaat cggtggccgg tccgcgccgg tggccgccct tgatcacgac
    38101 actctgggcg cccgagtcga cgattcgttg cgccgcccgg cggatgtctc tggcattggc
    38161 aatcgtcaag ccggccaatt ggtttgcctc gaggatgttc ggcgtgacca cggtcgcgat
    38221 cgggagcagt tgcgaacgaa gcgcgctgac ggcgtttttt tcgagcagca ggtctcccga
    38281 agtcgccacc atcaccggat cgaccactac gttggccagc ttgtgggcgc gaagcttttc
    38341 cgcgatgcat tcaatgatcg ccgcattggc taacatgccg gtctttaccg cgtgggcgcc
    38401 gatgtcatcg atgaccgcat cgatttgcgc cgcgacaatt tttggactga gctcgacaat
    38461 ggcggtcact cccacagtat tttgcgcggt aatcgcggta atcgccgaag tcccataaac
    38521 tcccagcgcg gcaaacgttt tcaaatcagc ttggattccg gcgccggctc ccgagtcgga
    38581 gccggcgatg gttagagctt tgtagatttg cgttttcatt gatcggcatc gtagcaaagc
    38641 tcacaggaag ttacgatgca tgtgaaagga gcttcagcat agccggatgc gcgattcaaa
    38701 cccacctcaa tctgcgtgac gctcggggca cggagccgac gcagctcgat tgatatgaac
    38761 ctttttgttc aatcgttcgt aaacattttc tacgaacggc aacttttgtt ccattcgggt
    38821 cgaacacgtt agatacaaat cctaaatttt atgcggttaa ttcgcagtcc tgtttcctgc
    38881 agctcctggc acatgcgttg cttttacgag ccagtaagag gtcatcgagt caaactgcac
    38941 gactgtcaaa cgaacgaaaa aggttgtggt ttatgaacga atttttactc ctggcattgg
    39001 tcgccatcta tctcacaatc tcatatccgc ccaagtccaa acagcccaac agcaacgata
    39061 gcgaatggac gcggcgattg ttcgctttta tcggaaagaa acgcgcttga ccttgccctc
    39121 gggtttgagt cagcgagatc gtggtatcat acggattcga tagaccaaga aagctccagc
    39181 ttccctccgt tctgaatcct gttcttaatt ccatctttcg ttttgctgca gactaggttc
    39241 cctgatatga gccgatgagc gatccggagt tctccggact cctgagattc atcttagcgg
    39301 caaaaaggtc tatctaatag gtgaggcaaa ataggtgagg caaacggcta atcagccgcg
    39361 gcctgaacgg gtgcgctggt gagcgggaat tctaccaatg tcgctaaagg aaaaagcttc
    39421 tccagctagc tttctcaact atacgccggt gccgctggcc tttggcacca gcggcttacg
    39481 cggtctggtc aaagacatta ccgatctcga ggcttacatc aatgtcaaag gtgcgctgcg
    39541 atacctcctg agcattggcg acatggcggc accccgcaaa gtcgttctgg ctggtgatct
    39601 gcgcccgagc accgatcgca ttatgagagc ctgtatgcag gcgatgcttg actgcggttg
    39661 ttctgtcgag aatgccggca agattccgac gccggcgttg atttcacgcg cgatggccag
    39721 ccggtgtgcg ggggtgatgg taagcggcag ccatatccca ttcgatcgta acggcattaa
    39781 aatcaacaag agcattgggg aaatactcaa agcagacgag ccgggcattc ttcgtgaggt
    39841 ggagcgcgtc cgggaggaag agtataaccg gacggcggcg acatcagcat tcaatgccga
    39901 cgggatgctc aagcaagcgc cgaatttacc gccactcgat cgccgggcgg aggaagctta
    39961 cgtccgccgt tacacggata gctttaacaa caacggcctg tcaggactgc gcgtgcttat
    40021 ttatcagcac tcggccgttg gccgtgacat tttgcctcgt atactgaccg aactaggcgc
    40081 cgcagtgatt gccgccggcc gcagcgatcg gttcatcccc atagacaccg aaaacatcag
    40141 cggcgaacag ttggatgatc tcgaaaaaat gatttccggc gttgaagccg atggccagcc
    40201 atttgaggct gttttatcca cagacggtga ttcggatcgg cccttggtgg tcgccgtgct
    40261 gcctaccgcc caagttcctc ggggcggtag gcgggtgcgg tttttgcccg gcgatctcgt
    40321 aggcatcatc actgccgaat atctcggagc ggatgcggtg gcggtgccgg tcagcgccaa
    40381 cgacgcggtg gagcgaagaa tggacgcgcg cggcattctg ctgcgtaaga caaaaatcgg
    40441 ctcgccctac gttgttgcgg cgcttgacga aatccgacta acgggaaagc gcgggcgagt
    40501 tgtcggctgg gaagccaatg gggggttttt aaccggcagc gatattagat tagccgcggg
    40561 cacactgagc gcgctgccga cgcgcgattc gacactaccg atcctggcca atctattcgc
    40621 cgcagccgag cgaaacatca gcctggccga attgtggaac ctgctgccgg ctcggttcgg
    40681 ctgcgccggt ttggtcgaca attttcctct ggcggcgagc caggcaattc tcacaaaact
    40741 gattccgccc ggcggattga tcgaagttga attcgacgcc gcgggagacg ttttcgatcg
    40801 cagcaccgcc gccaacaccg ctgcgccgct tgccccatct ctggcaagag attggcggca
    40861 cgccaaaatg accttgaacc gtttcttcac ccccgctcag ggcttcgatg acatcgctcg
    40921 gattaatgta ctcgacggcg tgcgcattta cttccgcaac ggcgatgttg cgcacgtgag
    40981 accatcgggg aacgcgccac agctacggct ctatgccaac agcgactccc aagcacgcgc
    41041 cgaggaaatc cgcgagctcg ctttgcgtga accggatggc attttgcgcc aattggagcg
    41101 agcgtttacc tgatgcgcca gcatggccgt tttttgccct tacaatccta gcgccggtct
    41161 caccttggtc gcaaacagct cgatggaccg ggtcacttga tcatgactta agccgccgaa
    41221 gctgaacatt cccactaggc tgtcgggctt tgcggtttca atcattgccc gcgctttggc
    41281 tagcaccgtc tgcggactgc cgatcagtga cgtgccccat tgatccaggc gcttgaccgt
    41341 cagcccgccg ggaaaatagg gaaaccgttc ggtaaacttc ggcaagtcgt ccgctgaaac
    41401 cgaatcaccg cgccagacat tatcggtagc cagctgccaa aaacgaacga gttgcggcac
    41461 cgcttcttcc cacgcctgtt cgtcggtttc cgcaacgtag atatcgcgca ccatgatggc
    41521 atcgccgttg cccgataaat ttttatgttt ggtccgttcg tcccgatagg atcggatggc
    41581 ctgggcacag ttggcgagcg gttggcgtga aagcgccagg ttccaaccgc gccgcgccac
    41641 catggcaggg ctgtcgggcg aagtggcgcc ataatagatc ggcggatgcg gacgctgcag
    41701 aggtcggggg cggcaaacga tatcgacgta attccaagcc ttaccgctgt aactgaaagt
    41761 ctcttcggtc caggagcgca tcatgatctc gattccttct tcgaagcgcg cctgcgccgt
    41821 gtcacggtcg agtccatgct ttaagtcatc acggggcacc ccccgtccca acccgacatt
    41881 caaccttccg cccgtgagga tgtccaacat gccgcactct tccgcgaggc gcatgggatc
    41941 gtatagcggc aggacgttaa ccagattgcc gagccgcagt cgtttggtcc ggtgcgccaa
    42001 tgaggcgatc atcaggttgg gtgacgcaat caggccgtag tccgagtggt ggtgttcggc
    42061 aaaccatgct tcatcaaagc cgagccgttc ggtcaagaga atttgttcga gcagttcttc
    42121 aaacgcttgt ttctccgaaa cccatggccg tgcctgatta taactaaatg tcccaaatcg
    42181 catccctgct cctcccgaga tgatcttgtg tgcaaatcta cccgcatcac ttcagtgaaa
    42241 ctcacccgtg cgcatctatc aagcggattc cggcttagcg gagtctacaa aaccgcgcgg
    42301 cgaacaggcc gccggccaat gtcgtgccca atctggatct gcccatcgca tcgccgctat
    42361 cgcaccgtca cttttccaat gcgatcacat gccgagccag ctctgaattg cgccggcctc
    42421 aaactgtatt cgcacgagcc attgaaagag cccataaaaa agcatcccgg tgattcccgt
    42481 cagagcgatc gacttggtcc agctcacttc gctttggaat tttagaaaaa agaacacaaa
    42541 cagcggaaca gtccacggga atcctactag atagaccgcc aggataaacg cggccatcca
    42601 ggagaacatc gtaataaccc gccggcgcgc aacttcgggc gggacttcat tggaaaactc
    42661 cgcctcgacc gcggggcctt tgttcctttc aggcgcgcca aacagttcca aatagcaatg
    42721 cagaagggtt aagccgagga gcgggatggc tacggctaag gggaagaaac cagttttaaa
    42781 cgaccactgc gatgcggcat aaatggcgta ccccgagacc aaaataagga aagtgccaaa
    42841 ataaaaatga cctttggact tcatcaatca ccatcgccgg accaagcagg cttctcgcgc
    42901 agaaagggat aaacgatcac caacaccatc agcgccatca aaactatcac gagcggatgg
    42961 gtcagccagt caaatccgta gcgcgccacc gaaatgaata gataagtttc caccagtttt
    43021 cccagaacaa gtcccaaaac gagtggcggg cgcggccagc cccaaatgac catcagatag
    43081 ccgagcacgc cgaagcagaa agtcacgatc aaatcggaga gttgtccatt ggcggtgtag
    43141 ctgccgacga agaccagtaa taatacaaac gggatgatga caccgcctct tatgtaagtt
    43201 accttggcga gttggttgag aacagccagc gacaggagga cggtgatgat attggcgatc
    43261 gctaatgtcc agaccatcga gtaggttacc gcgagatgtt tgctcaacat atcgggcccg
    43321 ggcaccagcc ccatgatcat aaatgcgccg agcaggattg ccatggctcc gctgccgggc
    43381 acgccgaatg cgatggtcgg cacgagatcg ccgccttctt tcgagttgtt cgccgctccc
    43441 ggcccgagca cgccgcgtac atcgcctttg ccgaagcgtt cccgatcctt ggaatctttc
    43501 gcgctctgca cggcgtgagc gtaggccatc cactgcgcca caccgccgcc cgctccgggc
    43561 atcacgccga cgaaaactcc cactgcggaa caacgaacca cgagccaaaa atgccgaaag
    43621 gtatctttga tgccttccat gactccacgc ccgaggtttt ccgcgggtcg atccccggca
    43681 atcgaagtgc cgcgcacggc cagatcgacg agctccggaa tcgcaaaaat tcccaccagc
    43741 accggcacca gatcgagacc ttcccagaga tacatcaatc ccatgtcaaa acggagcgag
    43801 ccggactgtc gctcctgacc tatggtggaa agcagaagac cgaaaagccc catcgcgaag
    43861 ccgcgaatct gcccgcgcaa acccaaaccg ctgagcgacg tgatgcaggc gatgccgatc
    43921 atcgagagga agaacagctc cggagaaccg aatgtcagaa ccaacgggcg gacgacgggg
    43981 atggctaatg ccagagcaac ggcgccgatg agcgcgccta ccagagaact catcagcgcg
    44041 gcgcccagcg cccggccggc ttcgccgttt ttcgccattg ggtaaccatc gactacagtg
    44101 gccgccgaga gaccttcgcc cggaacacca aaaagaatcg acgtgatatc gccagtcgtg
    44161 gccgcgacag aatgcatgcc gagtagaaat gcgaacgcct cggccgggct cattttaaat
    44221 acgaacggaa tcatcaacgc caaagtcgcc gcgccgccaa taccgggcag caggccgacg
    44281 caaaatccga ggcccatacc gaggagcatc aagctgaaag ctttccactg cagcacgagg
    44341 gacagaccgt cgatgagagc ctcgaacatg gttcagctac tccaattctc ttaccagagc
    44401 gcacttacta gcctaaagcg caaacgtttg cttcaggcac aacttgcggc gcgactatag
    44461 tgaatgacca acgacacgtc aaacaaaacc taagtttgaa cggttcaagc aggagaggtc
    44521 gcttaggata caatcgaaat acgtgtcagc caaaatcaca ccggagttat caaaattatt
    44581 gaccccgatc tcacgcgaat gttaagactc ggaagaggag cacaccatgg caaaactggt
    44641 tttagcagcg ggagttccgc atccgccgcg gctggtctac gagatggcgc aagcgccggg
    44701 aaaaacgcgc ggagaagcgc tcatgaagca ggttcgcgaa caggtggaaa aggccgagcc
    44761 cgatttgatc atcgaggttg acagcgatca tttcgtgaac tttttctata acaacttacc
    44821 atcgttttgt gtgggcatgg cggaggaagc cgaaggcccg caggagactt ggtgcccgat
    44881 gcctcgttat acggtgcgcg gcaacgtgcc gttggccaag gcactgttta aatacggggt
    44941 ccataatcga ttcgatcttg ccgccgccca cgagctgcgt ctggatcact cgttggtggt
    45001 gccgctgcac tttctcaatc cagggatgga gctcccggtg gtgccgatct ataccaacgg
    45061 ctttgcatcg cccttgccgt tggcgagtcg atgcttggct ctgggccgca tgctgcggaa
    45121 gtttatcgaa gcgtgggaag gcaaagagcg gatcgcgctc atcgccagcg gctgtttcgc
    45181 tatggatgtg ggcgggccgc tgcgcggctg gaccgataca gaatgggcgg aaacgatttc
    45241 ctcgctgctg ctcaaaggac gttaccgaac cttggctcat cgcgccaccg aagatcgcat
    45301 caacgccgcc ggcaacaata gcggcgagct gctcaactgg atcaccgtta gcggcgcggt
    45361 cggcaggacc cggccgctgt ttttggaagg cgacgagggc aacggttatg cggtgtggaa
    45421 gttcgagtga ggttgattat tgttaatttc gtcgtcggtg acttcgcgtt tctcgatcac
    45481 tcgattcctt gcgcggcagg gggaggaatg aggtgtgagt taaggatagg cttgtcatgg
    45541 taacctatgc gctcgaacag cggtgtaaga cgaagccagc agcagggtgc cagactgaag
    45601 aaagagcatt acgatgagcg tctttcaagt caacaaactt ttatatctga ccgacaacga
    45661 cccggagctt cgcaaacgca tgttagcgga tccggaagcg gtcctgaggg agttccgatt
    45721 gacggcggag gaacgtgatg cgttgacttc aggcgcggtg gggaaacttt accagatggg
    45781 ggttcatacc tttcttctca atcatctcta tcgctaccag ctcttcggcg taaaccgcga
    45841 taactatcta gagcgcatcc gcgaaggcat gccttacgat ccgcgcttcg aactgggcaa
    45901 tatgccggtg cagcgattca tcaacaaata aactcattta gcgtagcttg tccgaacggc
    45961 ctgaaccggt gaaccttgca gcacacggca acgcttcgct caaaagttaa aaccgttcaa
    46021 gccgttcaat accttagaac agagatcgtg attcgcgccg tcagctttcc gccttgatat
    46081 acttgcccat ttctttcaaa gtttcaggcg tttggttgag tagcgcggtg aaatctttga
    46141 tcacttgctc gtgcgatatg aaactgacgt cgatcttcgc cttctgcgct tcggcgataa
    46201 gttccggatc gttgactgcc ttctgaagag cgtcgcgcaa tgtcgccacc cgatcggccg
    46261 gagtaccggg agctgcggca aaggcgcggc cgatggactg gggtatcgat ttgatcgcca
    46321 ttaaggattt tcccagcggg ctggtagcca gatcttcgtc cactggcaga ttctcaaacc
    46381 ccttgatcgc taccctgccg cgcaccagcg gccgaactac gccgcggtcg gcggcaagtt
    46441 tgatggttgt cgccaacgcc gaccatgagt cgacctcctt gcgctcgatg gctaataaca
    46501 catcgctgtt ggctgggtag ccggagacga gttttagctt gaccccggcg aattctttta
    46561 gcagcaacgg aatatcgtgg gcgttggatc cgggtccggt ggtgccggca accaactccc
    46621 ggtcagcctt tttcaactca tcgaaagtct tgtaagggag atccgatcgg atcgaaaaag
    46681 cactcccgtc cgccccggaa gaaccgagcc actgaaactt ccgcacatca aagcgaacct
    46741 gttccacttt cgacagttgc gcgatcgcca cgttgggatt ggcggcaagg atagtcagac
    46801 cgtcgggctt ggcgctgttg aagacgctgt tggttgccag caggtgcgcc gcgccgggca
    46861 tctgctgaac gatgacggcc ggtttgccgg gaatatgctt gcccagatgg tgcgcgacga
    46921 tttgggccgc gatggcaagg ctgccgctct tggcgccgat cacaacattg attgtttttc
    46981 cctcgtagaa gttggcttga cccgccgccg gcgcgacgca gaccaccaac caactcaaac
    47041 aggtgagagc tctcaatgcc ttatggtatc tcatagagtt tcctccaacg tttgctttcc
    47101 cgtaaacttt tccggcggtc atctcagggt ttctaccctg tcctttaacc gccgcaaggc
    47161 tatttgattg tgggtgttgg agtcaagcga gttttttgtg gatgaaagcc cgcgaggcgg
    47221 gatgccgtgg aaagctagtc attctagcat ggcagtgaag cccaatgtca tccggcaggg
    47281 ttgagaacgc gcactggcgg aattagcgaa attattttga cttcggggct ggatcgctgg
    47341 cgcgcgacaa ggtcgaagtg agaattttca ggttttcgct tttctcgatg tagcgcacga
    47401 acaggtactt gttttcctgc gaatccaaag ccgccgccgg gacgtcgccc gcgcccgctt
    47461 cgacttttgc caggatgaag taaagatcat tgctcttcaa ggcgcgtaag aatccttttt
    47521 cgaattcatc catggttctg gcggtaatca cattggcaaa gccggcgccg cgcgcaattg
    47581 ccgccaggtc agcctgcccg gctgttgcgg tcggggcgcc gcgcgaggat tcgtaacatt
    47641 cattgtcgaa gaccacgtga atcaaattct tcggatgctg attggccatg gttgctaggg
    47701 caccaaggtt tagcagcaaa gagccgtcgc catcgaaggc gatgaccttg cggtgcggca
    47761 gcgccaaagc cagaccgagt gccatggagg aggtgagtcc gaggttttgt agatggtagt
    47821 tggccggatg gggcctcagc gcttgccatt cgtggcgcac agcgccgata ttggtgacca
    47881 ccagttcttc gccgaattgc ggcgcgatgg cgcgaagaca atcgtatcgt ttcatcagac
    47941 tacctcagag cttgtttgtt gagcacaatg gcggtgggtc gccgcgagga gcgcgccagg
    48001 atctgactat cgcgaatcgt tggacggata tcgtcaaagc agccgacttt ttcatagaga
    48061 atatgcatcg cttgcaacac tggctcggtt aaacttccga cggttgcatg accggcgttt
    48121 tcttcgccga cgccgccgct gtgaccgatc agcatcaata ctgggatgcc tggatgaata
    48181 cagaccgtcg ccaaagcatt ggcggacaag aggaagccgg cgttcatcat caccatcgcc
    48241 ggttttttgc cgcccagata agcgcccgcg caaatgccga tcccttcttc ctcgcgagtg
    48301 accggcacat gatggatttc cgtatcctcg cccaaagcca tcaccaggtc atgcaaattt
    48361 gcttcggtta aggttgcgac gaagtcgatt ccggcttctt taagagaatc gacgatctcc
    48421 gccgctactt ctcgtttcat atcttcctcc tactgcattc gtttgcgtgc tcggttgagt
    48481 agctcgacgt cgatgtattt ctcggctttg ggcctgatct tgcctttgaa atcacctaac
    48541 tgctcgagga tgtcgagtac gacttcggtc ccgggaaccg tcattgcgcc atccgcgggg
    48601 atcactttat cgcgcaccca atactcataa actcgttccg catgatcgga ttgaactttg
    48661 aaaaaatcgc ggatgatcgt caacgttccg gagcggtttt cgtacacata gcggttggcg
    48721 cgcagcagag caagtaagta gtcctcggtg agtttgggat tttgatagat ccatggccgt
    48781 cgagccgaca gggcggtgaa ctgcacggct ggaatataat ctctcagctc acccaggatc
    48841 ttgaagccct gctcgactgc gacgtagctg atcggcggat tgagcacccc cgccgaggtg
    48901 gctccggtct tgatggctgc caggcgatcg ctggtgccgc cgacttccac gagtgtatag
    48961 tcgcgcggat aactaaggcc atgggcgctt agcatcttct gcagtagaag tgtgctcgag
    49021 gaattaattc cggaaactcc caatgtcgtg ccgcgcagct cttcgattga gcgaattttt
    49081 ttatcggcga ccagatcgta cgctgccgag ttggccaggc cggacagcat tgtcaagtca
    49141 gcgccgcgct cgaccgcgcg aatcagcgga tcgggagcct gcgcgctgag ttcgatagaa
    49201 ccggcgacca aagcttgaat ctgccgcgcc gctgaacgca tgactatgat ctcgttgaag
    49261 atctgttgtt ctttgaagaa accgcgccga tcggcaatgt aaagactcag attggtcatc
    49321 cccggcgaag tcaccgcgac gcggacgcgc cgcagttctt gcgcggcgac gggtacgagc
    49381 aaggcgaatt ggtgatggaa ggttaggaga acaacgatcc caagaagcca gcgtccatgc
    49441 atacggcaat tcctgctttg ctatactcaa gccaaggcga tgacgcaaga gatttatatg
    49501 tgtgtgccgt cgcgcgcgct ggtttttcga agcagtttct accggtataa gaaagatatg
    49561 gccaggcgct gtgatcaagc cgttctacgg ctctaaggtt ggcgattaat cccgccagtg
    49621 cgcgatgatc tcgtcagatg aagcgatacg cccatagtgt ttccgcacca gcgcgaccgc
    49681 ctcattatgc gctcctggcc cgtaggtggt ggcggagcag tcttcaacca gggtgatgta
    49741 ataatccagc atgaagccga tcgttagggt atgaaacacg cacatgccgg tgccgactcc
    49801 ggtcaccagc aaactcttca ctccgcgagc gcgcaagagc atgtccagat cggtgccaat
    49861 aaacgcgctg taacggtgtt tcgtcacgat catctcgccc ggtcgcggtt ctaaaccggc
    49921 gtgaaattcg gcgccccaac tgccttcggc gcaagcgagc aagtctgggt ttttgaactc
    49981 ggtccagatc ggtgagttag tccattgatt gccggatgtg cgcacgaagc aaattggaaa
    50041 gttgtgcgcg cgggcttgct ccaccagtcg aatgagccgg ggaatcatat tctgcacgaa
    50101 atccatgtcg cgcccccgct tgcaggcggc accttgacga tgacagaagt cattctgcat
    50161 atcgacgaca atcagcatgc cgtgagttga atcgatgcga tcttcaaggg acattgccat
    50221 ttccacctcc tattcgtaga gcttcttgat aaaaccgctg tcgtccagtt ccttgacgat
    50281 gctggcgtca atcaccgact ttggatcggc gctccgggcg cgcggattgt ccttggcgag
    50341 aaattcgagg acgctgctgg ctcctttaag cgaagggtac ggcgcattct cgaagtagcc
    50401 ggaagcataa agcgcgtacg agcgttccag atattttgtc tgcgccggcg cagtgcgaag
    50461 tttcttcttc atgacctcga ccgattcggc ccgatttttc ttgaagtagg caatcccctc
    50521 gacatacgcc ttgatgaagc gagtggcgag gtcgtggtgt gatcgcaggt aggagcgggt
    50581 cgtgtcgatc atgctgtgca ggtaatagat atccatatcg gcgaggtcgg cgagcacgcg
    50641 gtaaccaaga tcttcggcga tgaaaaaagt cggctctgtc aggaccgccg catcgatgcc
    50701 gcccttctcc agagccgcca tcatcgccgg agacgagcca acttgaatgg cttgcacgtc
    50761 agtggaattc attttccagg cacgcaacat cagcagcaga accatatgcg acgatgcgcc
    50821 taatcgggtg acgccgatgc gtttgccttt gagatcttcg gggcgcttca gatcagcgcg
    50881 cgccatcact ctctgaacgc ctttattgac aatggaggca accattacga catcggcgcc
    50941 gttgagcgcg gcattgataa cagcgccgcc gccgccggtc agaaattgaa tgtctccggc
    51001 aatcaatgag ctgacgccac ccgcggaagc gccgccgacg acgatcactt cgggatcgat
    51061 gccgtacttc ttgaatagcc ccttctcttc ggcgatccac acggcagcgt tgatcggagt
    51121 aaacgagctc aagccgatgc gcaggcgatc ggcagccacc gcggggctta aaagccagca
    51181 cgacaggatg agtcctgcgg tgctgacacc cgcgagagat ttaaagagca catgagtcat
    51241 gatcgtcacc tccgcattaa atcacgctct accctgtgac ccgtaggcgg tcttatcact
    51301 ccgcggaaaa tgcgtccact attgcattat ggttacccac ctactccgct tgcctgattt
    51361 cgaaatgcgc gtctcaagcc ggcgtggcgc gttcgccata tgcatcctgt tcaatgcgaa
    51421 tctgggggga acttactggt aatacactga agaaaatagt tctcggacct aatgctcgaa
    51481 tccgtgctca agataagcag caatgtgatt tggacgatgt gcgaatccaa gtgtagggga
    51541 ttcgcagcgt catctaaggc gacggcttaa gacgcgcgcg ctgagaagcg cgaagcggct
    51601 ttctcaaacc ccttgttagc tcacgcgttt gctctggcag gtcttctaat cggatacgcc
    51661 cggcttttaa gcttcttcga gcttgttcga ctcgctcaag gaagcgcgga tcgttctcaa
    51721 gcttgtaatc aaaccagtcg ttgaccatat cggagattga acgatccgat gccgcggcct
    51781 tcaaacgtag ggcgcgttaa acgtcgggct cgaagtaaac agtagcgcgt tttttgatta
    51841 tccgtagtga cattccggcg ttctgacgtc gaaacgtcaa gacgctcgac actatctgac
    51901 tactagttgt agcgaccacg ggttatcggc agcttatgct cgacagttct ttgtggattt
    51961 caaataatta ctgtgcccca aaccgtgtta cttgccaacg tcaatcacgc ctgtccagaa
    52021 atgccgcggc tatggcgtct tatcgcactc gtgtcggaac ccctcggccg catcgccgtg
    52081 catcagtgtt tatcttccgg cttgacgtat ttgactgtgc cgccgccttt ttgcgaatgc
    52141 agataacctt cgacgtcgct caatggattg gtgtagtagc gcatgcggtg cgacaccaaa
    52201 tcatccgctt tcaaagccat ttcaaaagaa agatctttct gcatataggc tgcgatcttg
    52261 gcgttctcta aagttgcgcc gttgcggctg gccatttcct tcaccaaggt gtcgatgtat
    52321 gagtcgagct ctttttccgg cacgacctga gatatcaagc cgatccgttc ggcttcgatc
    52381 ccgtttagat ttcgcccggt aagtgaaatg aagaaggctt tcttgaacgg aatgcctgcg
    52441 tggaacaatg tcggggtggc ggtggcgccg tagctgccgc ggatgacttc cggcatgccg
    52501 aactgggcgg tatcggcggc gataacgata tcgtggccat tgatcatcgc taggccgccg
    52561 ccaagacacc agcctctgac tacggcgatc gttacctgcg gcagtttgcg catgatgtcg
    52621 acgatttccg caaccccacc gatgccgcga gtacggcgac ggttttcctg gcctctcata
    52681 tcgtaaagat cgcgacccga agagtacgtg ccaccggcac cgctggtgac gatgcagcgg
    52741 atcttgtcgt tgttggccag agagcgaaga atgccggcaa gctcgctcag tagaacgatg
    52801 ctcagcgcgt tttttttctc cggccggttg agagtcaacc aggcaatgcc ctcggtttcg
    52861 ttaatgtcgg cgatgatcag tttgttgtcg gacataaaaa ctccttcgta agatcgttca
    52921 acgttcaatg tcaacggtta ggtcgttcaa aacgttcaag ccgttcaaga tgttcgcccg
    52981 ttcggccgga ctcaagagag gcggtaaaag gctttcgcgt tatccgcaag gattctttgt
    53041 ttggttccgt ccgaaatgtc tttacgccgg actacttggc tgacgctgtg cggccattcc
    53101 atgtcccagt gcggataatc cgaggcatac atcatgcact gatcgccaaa ctgttcgatt
    53161 accaacggca atgccgactc ctcgccttcg caggtgaaat agcagcggtt gtttttaacg
    53221 tactcgctgg gcaacatctt gagcggctcc atctcggatt tgcgtctgag atatttttca
    53281 tcgaagcgct ccatccaata ggggagccag ccgacgccgg cttcgagaaa cgcgactttc
    53341 aatttcggaa agcggtccag cacgccattg gaaatgatcg ccacaatcgt cagcatctgc
    53401 tcgaacggat gggaggtcat gtgcacgcca agatatttgt gaaagcgcca gttgccttgc
    53461 gagacctgcg cggtggcgtg aaagccgata ggcacaccga gctcctgagc ttccgcatag
    53521 atgggatcga actccggttg gcccaggtcc atacccgatt gcacgtaagt cggcaccatc
    53581 acggctaccg caccgagttc ttgcacactg cggcggatct ccttcaccgc ttcaggcaca
    53641 acttgcggcg cgatcacggc agcgtattta actcgattgc cgctctgctt gcagaaatcg
    53701 caagcccagt tgttgtatgc cttggccaac tcaatcacca agtcgagctc gcgtaccagg
    53761 ccgatggaca gcgcgttgct aggataagcg acggcatcgc cgatcttctc cacatccaga
    53821 tccttgcagt actcctgggg cgtggccggc ttcctgccgg ttgtatcgtt aagaaagata
    53881 tcccagccga aggcagggaa atacggcccg cttcttatgc gaaaacgttc cggcaaatag
    53941 ttcttataag cgggcccatc gaggtgatgc gcatcggcgt cgatcacagt aaagtccacc
    54001 aattaacctc ctaaactatc ggctgttcaa aatgtcaaat tgtttcaaac cgttcaaaac
    54061 cttaggcctt gaccatcccg gggtgccttg gactctctga gattgaacgc ttgaacggtc
    54121 ttatctttta atcctgcagc ccatagcgat gcgcgatgtt gagaaagatc gaaccgacga
    54181 tcacagtgcc gatggtgatg attaggctga aggccgccaa ctcgccgaca tggccgttgt
    54241 cccatagctc ccagatggtg accgcaacca cttgcgatcc gggcgagtag agcaacagtg
    54301 caatggataa ttccttaaac gtaattagaa agatataaat ccagccggcg agcagcgcgg
    54361 gcataatcag cggcacgacc acgtgccgga ccatttggaa ccagctggcc ccgctggtag
    54421 tggcgccctc ctcgagttct ttatgcaggc ttaaaagcgc cgaatggctg aagcggattc
    54481 cataaggcag atagcgcgcg atgaatgcca gcaccaggat ccagatcgtg ccataaaccg
    54541 gcaccggcag gatgagatac atcttaagaa tcgctatgcc catgacaatg ccggggaaaa
    54601 ccagcggcag catggccatc tggtccagta gccaacgcca tttgattgac gcgcggacga
    54661 tcaaccaggc ggcaaagaag gtaatgagca cggcaaccgt cgccgagctg atgctcaccg
    54721 tcaagctgtt cagcaccgag tgaatgatac tgctatcggc aaacgcggtg gcataattgt
    54781 tgagcgtcaa catcgagaat gctttgcgcg acggcacttg agcgaacggc aggaacgagg
    54841 accaggcgat ggccgcgatg ggcacgaact ggagcaacgg taaagccagc atcaagaagc
    54901 cgcccaacca gcgccatttg ccgagctcga tacggttcgg gcgaaagttt ttgccgctga
    54961 tggtggtgaa tttataagtc ttactggtga tgcggtaata ggggatcagg cccaggccca
    55021 ctagaacgat gagaatgatg gaataggcgc tggcttcgcc atacttggga atgagcccgc
    55081 cttttatttg caaatagatt ttcgtcgtga gcacttcgac gccggcggga ataccgatca
    55141 gcgccgggat ctcgaaagct tcgagcgagc ggatgaaagt cagtatcaga actgccaaga
    55201 cgctgggcaa tgccagcgga aaagtcaccc gccggaaaac ctgccagccg ctgctacctg
    55261 ccgtggtggc ggcttcttcc agcgatggat ccatggaacg aaacggcgtc gacatcagca
    55321 ggaacacgat cggaatccac agaaaacttt cgaccaggat cattccactc attgagaata
    55381 tattgaacaa aggttggccg gtccaggcaa cggccggagc attgagaatt cccgccttgg
    55441 gccccaggag catgatccag ccgacgactt tgatgatgcc cggaatggca aacgatacgt
    55501 aggcggaaac gtaggcgagc ttgcgaaacg gcgcattgct gcgctcggcc agccaagcca
    55561 ataccgttcc ataaaccaga gcgatgccgg cgctgccgac cgagaagatc atcgaattgg
    55621 ccagcagagt tttaacgtcg cccaaggaat cgatgatatt ggtaaaatgc tggatagtga
    55681 aactacccgc ttgcaggccg cggtccgtca ccaggctggt gtgcaaaatg aagaagaacg
    55741 gcggcagcac caggtaggct aaggtcacgc tggttaaaag aaacagccac tgggctggat
    55801 tgcgcatcat catggcaaac ataggcaggc cctcttaggc tcctagagtt tctttttcct
    55861 tcttcctatt tcaatggttg agaaggagca acttgagtta acgaccactg ctttgcgtct
    55921 ctcctcgcta attgaagtgg aaccgctgag cgcattacct gcggctgact tctttgagca
    55981 gtttctccca acgctcgagt tctttttcgt acttctcggt gctgattcca tggtccggcc
    56041 gccagcgttt gaaaccgaga tccttggtgg cgctgccata ctggtacttt gccagaagcg
    56101 cttgcccttc ggctcccaag agataatccg ccatcagcag cgcggcgtga ggatgcggcg
    56161 gttgcgcggc aattgcggcg ccgcccacat tagttggcac gagatccaac ggcacccact
    56221 ccaccggcgc gcctttggcg accgcaacca gcacgtgatt acgaaagata cccggcgagg
    56281 ctaatacttc gcccgaggcg atcaggtcga gcaaggccgg cggaataatc gtatgcagcg
    56341 cgatctcctg gaccttgagt ttccggacaa actcctggcc cttggtcttt agcatggcgc
    56401 cgatcacttt gtagatcgga tcgctcagcg atacacccat gcgccctttg aggtcaggat
    56461 gaagcagccc ttcgaaattt ttcggcactg ctcccttgga gagcaggttc ttgttatagg
    56521 caaagccgat atacgattca cgggcgatcg cccaaaaata cagaccccgc tcacccttct
    56581 ctttggcttc ttccggatac gaggcaaaat ggggcgaatg atagggccgc aggagaaaag
    56641 catcgcgcag gaacataaga ttgccttcgg tggtttccac cgtgtcggca atgaatcttt
    56701 tcgctttcga ttcctcgaac attcttgtag tgatgtccga gcccgagact cgataggctt
    56761 cgatcttgac gccggaatac ttgctttcga atgctttgac catcgctttg taagagtcac
    56821 ctgccagtga ggtataccag gtcagcttgc cctcgttttt tgcgccgtcg tacaaaacct
    56881 gttcacgatc cgcgccgttg taagtcacca gctccgaaat cgtcgccggc ttgcgggctt
    56941 gtgcgcgcag tgtcgcggga aacagcataa aaaccaacag caaaacaccc agtattttca
    57001 ttgaccctac ttgcctcccc ttacggttct tacctctcac ttgccaaact gacaagcgcc
    57061 atgcgctgtg ttcctatgaa ttgtcgtaag ctcggtatgc ttccgagtcc ctgccagcgg
    57121 cgtgattgct cgcgcctaac agaactgggc cgaagcttcc agccaatctg cggctgccaa
    57181 atctacggcc catcgagggt gaatgcaata ctgctcggtg agggccggac ggatggcaag
    57241 ggcggcggtc ggacctatgt tcggcaaaga acagatcttt gccgggcata ggtgaatttg
    57301 cgcctgctgt cgaggaattg aagattactt gttcacctgg tctatgtact gccggatgaa
    57361 actgacgatc tcaggcggtg tttgtgaaac tttttgaaac aaccgctcgc cctgctcgcc
    57421 gacttcgaaa cgcggttgaa aacgcatcgc cttcatcgcc tcggcattga agtcggaatc
    57481 tttggagagg gacacaatag cctgcctgag gatcgcgatg gtttcggcgg gcgagtttgg
    57541 cggcaacatg atcaggcgcg tcatgttcgt gctcgacatg ttggccgctt tgagcgcttc
    57601 atactttatt cccgagggca ttttaccaaa cacttgccgg taatagtggg tgaagggcaa
    57661 caactccggc acgtccgggc tgacgatgac ttctccatcg ggcgaaacca aatcggtgta
    57721 gtaaagcggg gtcacttcac cggttttaac catctgcggt tcgacggtgg cccggtaagc
    57781 gggaagagtt tcgtcgtgat attgggcctc attgcgttga acggccaatc gagcggttga
    57841 gctggaactg taacccgtca cataatcata cttgagcccg agcaggtcga acgagagccg
    57901 gaagcgcacg tctttcgagc tgctcgcgcc cagccccgcg gccctaaacc gttccgcttt
    57961 cacgaagtct gccggtttct tgattcccgg cggtgtgtca gctcgtatat aagacaccgt
    58021 aacgccgtgc acaccggtga taaacccgaa cttgccgaga tcgacacgga gccccgggtc
    58081 cttcgtttgc tggtgaaaca ggctgccggt gaagtagccg gccgtcagcc catccggttt
    58141 ggcgacttct ccgatataat tgactcctgt gatgccgccg ccaccgccca tgttttgaat
    58201 cacgactgcg ggctgccccg ggatatgtct gcccagatgc ttgccgaaaa ctcgcccttc
    58261 gatatccgta gggccgccgg cggcaaagtt gatcacgatg gttaaagtct ttccctgata
    58321 atagggcgcc ttttcagccg cgaatgcttg ggtccatgcc gcaatgaaaa aagagcagcc
    58381 gataagccgg aagaacagtc gcaagttatt cgttctcact gcgattcctt tcgtgatgag
    58441 ttgatccaca agagcgctaa tgttcacaac aaggtacggc tatagagctg gtatgatttt
    58501 atcgcctgaa tacctctaaa tttcttgccc gcattactac cgcaaccggc ttcgggttgt
    58561 ctatctcttt tgttgaggtg atcaagggac agccggcggt cattattcgg cttgctgctg
    58621 aacctcacga gggcgagagc agtttactca tccaaacttg attgggaagc taaccattct
    58681 tgccagtgat cgcattggaa cacgccggcc cgcgtggtgg aaattatttt ccattggtca
    58741 tgacgaacag cagcggaatc cttcgacagc agtcagaaag actaaacgag ccctcaaaat
    58801 ggcagatgag atgctcttcg attgtaccgc tcggctatat tcaaacccac aggctcagat
    58861 tgaagaattc agacggctta gcctcttgga tacaacgcat ccatcatcaa tcgtttgtgg
    58921 tgcccgggag tgccgagata ctggtaaagc gtgtgggcta gcacgctatg cgccatcatc
    58981 ggatggccat aatcggttcc cggtccggcc cacaccatgt gcgcatagtt ggtgccgtga
    59041 taatagccat cgctcgcaac ggccttggac tcgtgaaccg cggcgcgcgc cggcatgccg
    59101 ctatcgatgc gccagattgc ttcgtaagtc gcccagcgcg cctgatcgag atgaattgaa
    59161 atatcgacgc aatggtcttg cacccgctgg aagcgcccga tcggttggcc gaaaaccacc
    59221 cgggtgcggg tatactcgca ggtcatgtcg aaaacttctt gcgtggcccc cacttgatag
    59281 gcggatagaa ccggcaaggt tttctcgagg gccgcttcca agatagccca actcgcgccg
    59341 ctcgacccga gcaaatggct cggcgatacg atcactcggt cgaaacgcac ctcggcgacc
    59401 gagataaaaa agcccacgtg aggcgtgatc gtgattccgg ggctcttcgc atcgaccagc
    59461 acgaaaacga cttttccttc ttcgctgcgg gcggcgcaaa gaaatgaggt tgcggcttcg
    59521 gcatcgaaga caaagcgttt ggtcccgctc atgatgtagc ggtcggaaag cttggtgagg
    59581 cgcgtttcga cggcttccgg tccccagtaa gccgctttat cggtaatcgc cggcacgacg
    59641 atcgcttcgc ctttgcatag cgatggcaac aaggattttc tttgctcgtc ggtgcacgct
    59701 tcaaaaatca tctgcgcgct gagaatgccg gatgtgaaaa gcggccccgg caccgggccg
    59761 cgcccgagct cttcgaaaac cacggcgcaa tccatcaccg acaagccggc gccgccatgt
    59821 tcttccggca gcatcatgcc gagccagccc atctcggcgg cttttttgat cagctgcggc
    59881 tgaaaggtgt ttttgttctt gtaccattgg gtcatctggt gcgccggcac ttcggccttg
    59941 acaaaatccg ccgcaacctt tttcaacatt tcttgatctt cggtcaatga taagtccatc
    60001 gtctgtctcc tattggaaca aatggaacga ttggaacttt tggaacgagc agttgctgtt
    60061 ttgctccagc cgttccagtc gttccaaacg ttcgacatgt gggtacgaac agttttgcat
    60121 gtggtttaat ccgtttccgg cgccttctcc gtggtcggcc ggcccaggcc gagacggcgg
    60181 gcgaagatca agcggtcggt atccaatgtg ccgccgccgt gcaactgtcc cgggcccgtg
    60241 cgcacggcgt attcgaaatc gacgacttcg ttgaccgata aattttgctc caacgcgtct
    60301 gcgcccatga tcgcttgcag gcgttcgccg ttacgcaggc gcagcatacg ctggtagtaa
    60361 cgctgctgtg gcccgccgta gggatgcggt ttctttgcga agcggcgcca atagatgagc
    60421 tggttgaagc gcctgagcgt gttcaactcg atggtcatct cggcaatcag atcgcgcacg
    60481 cgcggatctt ccatgatcgg tttgccgtcg atatgcgtat tgcggcagta ttcgaccact
    60541 ctttcgatca gaggatccgc ggcgatccgg ccgtcgcccc catgttcgag ctccatgtgg
    60601 gtgctggcga ctttccagcc gttgttctcg ccaccgatca aatatttcgc cggcacccgg
    60661 acattgtcga agaagacaga gttcttgatg cccatcatca tatgcatgtg ctggatcgtg
    60721 atacccggaa gatttgccgg gacgtggagc caggcaaggt tttcatggcg cttgccaccg
    60781 ggcgtggtgc agaccagcgt ccagagataa tccggcggca gatgatggcc gaccatgact
    60841 ttctggccgt tgacgacata aacatcgccg tcgcgaatcg ccttggtctg gcaattggcg
    60901 atgtcgctgc cgccgtgcgg ctcggtgagc acctgccaga cgcaaatttg cgcccgggtg
    60961 atcggctcga gaaattcgct tttttgctct tcggttcccc agcggatcag caccggcgcg
    61021 acaatacgcg ccaaggtgta gaatacgtgc gagagattga gcccatactt gaacagctcg
    61081 gtctcgagca ccatctgatg atcgatggtc aggtcggcgc cgccgtattt tttgggatag
    61141 atcggaaaca accatccttt ggcgccgagc ttttcggcca gcccgcggcg aaacttatat
    61201 tcgtcttggt cctcgcgggt cgaccacgag gccgaccagc gcagctgctc gtggccggca
    61261 aactgttcgg ccatccaggc ggcaacttct tttctgaatt caacgacgtg gggtggatcg
    61321 gcagagacat taaaatccat atatttctcc ttttttaggg cgtcccaaaa cgcccacctg
    61381 ctttgttgcg ctcgtcccgt tcgcttcagc gtacctccag agtacgcttc cgctcacggt
    61441 ccgtcgcgcg ccgcgcatct ggacgttttt gaacgcccta gtatattttc acccgagcaa
    61501 aaccaattaa atcactcgat cattgcggag ctctttgaga gctgtcttgt catatcccaa
    61561 ttgtcccagc acttcttcgg tgtgttcgcc gagcagcggc gcgggtcgat aaaatcgcgc
    61621 cggcgttccg gatagattga tcgggctgcc gaccagctgg gtcacgccca tttgcgggtg
    61681 cgtaatctgc cggggaatac ccaggtgctt cacctgcgga ttctcgaata cctcctggat
    61741 ggtgttgatc ggcgcgttgg gaatgctgtt ggcgtccaat cgccgcaacc agtgatcgcg
    61801 cgctttcccg gcaaactgct ggcggagaat cttgacgatc tcctcgtgcc ggtcggtgcg
    61861 ctgcttgcgg gaagcaaagc gcggatcgcc gatcaatgaa tccagctccg ccgcttcgag
    61921 caatccttcc cacgcctggt tgtgccccga gaggtgaacc atgaagggct tcttgtcgca
    61981 gtccagcaga ctatagatcc gccccttggg aaaattctca cgcgagaccg cttcgccgcc
    62041 gtttagataa tcggccacgt gcgattcgat gaaagcggtg gccacctgca gaagcgacac
    62101 gtcgatgaac tggcctatgc cggttttcac gcgtgccaga agggcggccg taatgccgtg
    62161 aacagagaag atgccggcgc agtggtccac gaacgagatg ccggcgactt tcggatcatc
    62221 gagatccgtg accaagctca tcatgccgct gagcgcctga ccgattgtgt cgaatccggg
    62281 cttgtcgcga tagggaccgc tctggccgta gccggagatc gaacaatgaa ccagtttggg
    62341 attgatcttc gccaggtctt gatagtcgag ccctagttta gttctcgttg gcgggcggaa
    62401 attttccaga aagatgtcag ccgtggtgag cagcttaaac aaaatctcgc ggcctttttt
    62461 ctgccccaga tccagtgtga cgcttttctt gttccgatta agcgcgcgaa agctcggccc
    62521 atagccttcg tattttggat tgcgcccgcg gtgggtatcg ccgccgccag gcagttcgac
    62581 tttgatgacg tcacagccga gatcgcccaa gaggcttccg gcatagggcc cggccacgtg
    62641 accgctaaca tcgatacaaa caaaaccttc caaggctcca gaaatcatgc gcgttttaaa
    62701 aatttcaagc tcaagcgatt catctatcgc aatcgagaat tgttttccac cgccaggcca
    62761 agcacgatcg ttaagatcag gcgagtttcc gacttctatt gcgaaatgtt aacgatggcg
    62821 gctgtacatt tgccgcgaag aatgtgccat cacaatcgtg cagcgggaaa cacctaaatc
    62881 ttcgagctcg agatactgaa ttttagcgcc atacttcatg agaccaggtc agcgccgagt
    62941 atgcctcagg aacccgggat gatcctcgac ctgatcggcg ttcacgattt gttcgccaac
    63001 atttgtcgct cggcgaatct tgcctttgaa ccgagccctc cggcatacag ggctgcactc
    63061 agcccggcca tgcgccgccg ggaatgacga tgtcatacgg attcatgacg ctacttgtag
    63121 aggctgtcga tgtagccgga gcggtcaagc tcttcgataa accgcgtgtt gacaaattcc
    63181 ttcggattag cggtttttgc cgcgggaact cttttcgaca ggtcgtcgaa gatcgtctta
    63241 aaaccatcga gcgtcggata gggcttgatc ggcacgaaac tcttgagcag caagtacgta
    63301 tcgtcgatct ctttttcatc tttgatctgc atgcgcctcg acaacgcacg ctttgccaca
    63361 gttggatcag tacgcatcag atgaatccct tcgatcagcg acttcacaac gcggcgcacg
    63421 aggtcaggat ttttcgagat aaatttatca gtcgtgttca ggccggtttg ctgtacaggg
    63481 atatccatct cctgcatatt caccaaaact cgcataccgg acgcaagggc ttttagataa
    63541 taaggcggct gaatgatcgc tccgtctatg gtcttgcccg cggtgagaga cgctagaagg
    63601 atcgggacat cgccgacctg aatataggag acgtccttat ccggatccaa tcctagtttc
    63661 ccaaccagga gccgggtcat cgaatccgta acggtgccga aacgcgtgat cccgagtctc
    63721 ttacccttga gatccgcaac agtcttgatc tccgggcgca ctaccagccg gtagaggggc
    63781 ttgtcgagaa agctggtcac catgactacg tcggcaccct gcagcctagc ggatgaaagt
    63841 aaactgccgg agtacacgca catgtccact tcacctgcaa tcattgccgt ctggccaagt
    63901 gtcgtccgca ggtagaggat ttgcgggtcg ataccgtttt tgtgaaatag cttgccctcg
    63961 tcggtgaccc atagcatcgc gttgctgacc gtcacgccgg aatagccaat gcggatcctg
    64021 tcggcggcat gtgctactcc gcacaccagc gcagccagca gaatcaaaga tagtacgccg
    64081 atgccgcgtt tacttgtaaa gactgtcgat ataaccactt tgatcaagct cctcgatgaa
    64141 ttttggctcg gaaaaatcct tgggatcggc cgttttcgcg gcaggcattc tgttcatcag
    64201 actgctcagg atcgcgcgga atccgtccag gctgggatag ggtttcggtc gggtgaatcc
    64261 attcaacaat tggtacgtct ctgccaattc gctttcgttc ttgctgtgca gacgtgcaga
    64321 caaggcgcgt ttggctatga ggggttcttt ccgcagcagc gctgcgcctt ccactatgga
    64381 ttttacaacg cgccgggtga tatccggtcg gctgctgata tattttccgg tggttacgag
    64441 gccggcatgt tggtagggaa tgatcgtttc ttcaagatta aacatgacac ggccaccggc
    64501 ttcgacggcc tttctatatt ccggcggatc gaagatcgcg ccatcgatgc tcttggcttg
    64561 aagggcaact aggcgagtcg gattgtcgcc aatttggaga atggaaacgt ccttgtcagg
    64621 attcaggcct agtttcgaaa ccattaaccg catgccaaaa tccgaggctg tgccgaaacg
    64681 agtgatgcca agtcgttttc ccctgagatc cgcgaccgac ctgatgtcgg gccggacaac
    64741 gaggcgggac aacaaatggt ccaggaaact tacgaccatg gtaacatccg ctccttgcag
    64801 ccgagccggt gtcatcagca gtccggtgta cacggcgaat tgagtttctc cggcaatcaa
    64861 cgcgctttgt cccaaggaac ccgcgagata gataacctcc acatccaatc cgtttttctc
    64921 gaagagtttc ccttccttgc ttacccaaag aagggcgttg ctgatcgagg gtcctgaata
    64981 actgatacga agcctgtcgg cggcgaggct tgtgtcgctc aagaagagca ggacaaatat
    65041 gaatggaaaa acaatccttc ttttggcggt gatcataaac tcgatagctc cccggttcgc
    65101 aaccacggct gaaggtcgct gcggaattga atgatgccaa ggctgataat gttgcggctt
    65161 agcgccagag gtaaaagctg ggcatgactc tgaacaaaca acaatcggcg tgccagctat
    65221 ttgacagata tcgttgtacg ttggtcgata tgcataacgc gaacgacacc tacgaacgcg
    65281 cggtcaatcg aactaaagtt cagcaacgat agcgatctgt agccttcaca caatctcggc
    65341 tattcaccga cggccttggt caccgagcgt gtcgagccgc cttcgggagt gctctgaccc
    65401 cacacgtgga tatactgcga ttgagagccg tctccgcccg ctacgaccac gataatatcc
    65461 tcagggtgat cggtgacagg aatgatactc tcacgggtaa gatttcggtg cgaccaacgc
    65521 ttgactcttt gattaaacgt ttcctcaggc aaatccttca tgcggtaggc tgctcgctgc
    65581 caaacaaact gctgcacatc tttcttactg aagccgtcat tggcaatgat ttttgcgtgc
    65641 tccgggcaga tcaccatgat cgcttcaggc cccgaaggtc cgtccatctt ttgagcaaaa
    65701 ttagtcagca cgccctctgc ggtggtgctg tcgagatcgg aagcgcgcac ggagccgtcg
    65761 gcgctgatcg ccgtgaccgt gctcgaattc ctgtcaaaac cgcgttcgac atgcagcggc
    65821 tcccacggac tagcctcttc gttctcaccc atgcagatgg agtacttgcc aggccaacct
    65881 agggtcgcct tgcaggtcgt ctctggatac gagccgccga tgttccgctg gatcagacga
    65941 atgcaacggc caatggttgc gttggcccgc cagccctcgc cgagcgcatt gtagccgccg
    66001 ttgacctgga tccgatgccg cacgggtccg ttgatgatca gcatcaccgc cggcccgccg
    66061 gtcgttgctt gaagtgcata gaggttcaac ttcgggtcgg tgatcgcttc cactgcagcg
    66121 atgaggacgg gcatgtattc gggcttgcaa cccgccatga cagcgttgat ggcaattttc
    66181 tccacggtgg ccgaggccca cttcggcggc actgtgccga tgacctcgtc cgctttgcgc
    66241 ttggtagcgg agagcatccg cgcgacgcga tcttccgtcg gcggaacgat gggcagtccg
    66301 tcactccaac ccttcgcttc aaaaaaatcg ttggcagccc agagttcatc gtcaatttca
    66361 atccgctccg actgaaatgc ttgttgtttc atgttcctta ttcccaacgt atgcgaacta
    66421 gaattacgaa agcgctcgag agtctccatt tgaggcttgg cgcgcatcga gtaaggcttc
    66481 gagcagcttc tcgaagacgg cgtcagcacg ctttttgact tcttcggctt tgaccgtgcc
    66541 gatcggatgc tgcacgatca caatcggcga atcgggagca ccgaaagctt cgcgctccga
    66601 cttcgccagc gcctcaaagg gatgagagat aacggtgacg gcgagccggc cgcgtttttg
    66661 tagttcaatg gaatcgtgga tactccacga tgtgcaggac cctcaatctc ccaatccgct
    66721 gatgacgaag tcggcgtgag cggcgagatc gtcgaggtac gctttgggcg cgccttgctg
    66781 ggcggtaagt ttgcggcgtc ttacaacgcc tgccacatcg caattctgtc tgagtaactg
    66841 ctcaaaacgt gcttgaagca tatcgttgtt gggcttgctg ttgtccaaca aacccaccgt
    66901 cttgccgtta agatctgcaa agcgtggcag cgcggcaatc cgttccgatg atgttcgcgc
    66961 ttttccttcc ggacttaata caatcatttg tttctccttg ttcggttcga tcgcattctt
    67021 tgacctattc tgagcggatt ctgtctcgtc gaaatcgact cttattggga ttttaccctc
    67081 tgtctaatgc ctatttgaat gatggttgtt gaattttttc cgataacgag acatcgatgc
    67141 gattctgctg cccgccctcg caactgggat aaccggcagg cgcgcgaacg ctggatacag
    67201 cttcctttat cgcaagcaaa aaatgttgtc taccggcgca atcactgtgc gctgcgcgct
    67261 cgggcgcgaa gaacttcatg cctggatgac cgcttgatca ccggcagccg cgcgggtggt
    67321 atgtttgatg agcagttgtc gtttatacga tctgtaaatt cttgttgcct gattgtttga
    67381 agatgtgaaa tattctgggc tcagaggagg gaagacgatg caagtgcttg atacagtatc
    67441 gattgaggat ctcgtggcgc taccgccgac gatctttggc gtcatccgat ggaagaaata
    67501 catcggcaga ccacaaggtg atctttgcag cggtttcaat attcgtgtgg aagagcatac
    67561 ggccactcag ttccgtgctg ttggcggtgg gaagtacgag ccgattcccg gcacgggtat
    67621 ctggaaagat gtggtggatg ccgtttcttg ccgtgagtta gcggatgaag gcgattacca
    67681 caagctcagc tttcaagtct ggggcgtcca tttgaatgct tttcccgacg ggcgatatcg
    67741 aatcacgccg actctgaaag gcacctggag ccggcccgcc aacccggttt tgttttttgg
    67801 ctctcgcact atcgagccgc agagcttctc tgtcgtgctc aagaaggatg atcaaattca
    67861 aagcgtcgag tttgaactcg tgcaacgtcc ccggccgttg ggaaccatct tgagcgcggc
    67921 cgttgtaggc gcgattgtcg gctcggcgct gcaaagtgca cggccgaata ggtggttgtc
    67981 ggccagatca ggaccgggta gctgagcggt ttctccaaag tatttccgat gcgaagaagt
    68041 ggaatttgtc ggctggtcag gacttgcaca tctccttccc aaagagtctc ggcttcattc
    68101 taataaaccg acatgccacg gggggtatta tgttagggag cattcgtccg gcgcttctga
    68161 ctctggtcat tgtgcttacg attgcgtacg cacccgcgta tgcgcagtcc ccggccgcgc
    68221 tgctccagca ggcggccgac gccatgggcg ggttatcagc gctgggcgcg ctaaagaccc
    68281 aagtgattga aagcgaaggc aaacagttcg atgcttcttc cacggcccga ccactcggtc
    68341 cgacccggca aatcaccacg tttcgctaca ccctgactcg cgatctcacc cgacctcggt
    68401 tgcggcttgt ttgggagggg caaagcctgg gcagcaagca aagcagccgc tttgttgaaa
    68461 tcatcgacgg ctccattggg atgctgcagg aaggtggaaa cggcgccaag caaacccgcc
    68521 tgcatcccgg gcgcttggca acgcgcgtac gcgaggagca ccgcaatccg gcaaagctcg
    68581 tcctgatcgg gttgaacaac aagagtttgc ggcaccgcgg cgacgccctg ctcgacggca
    68641 aacgccaggc ggttttgtcc tttactgaaa acgacgagga gtttcgcgtc tacgtcgatc
    68701 gcgagacgcg gctgcctgcg caaaccgaaa ttctcgagga cgatccgctc gaaggcgaca
    68761 gcagctatac attgcgctac ggcgattggc gtaaagtcga cggcgtgatg ctgccgttta
    68821 gtttgcgcta cgagctcaac ggcaacacgc tgcaggagga gcagattaaa tcgattaggc
    68881 acaatgcggc aattcccgcc gacatgttcg ccgttccgga ctccgtgcgc cgcgaaaaaa
    68941 ccgatgccat gccgatcgcc tcgcaatgga ttctgcgtcg cgtcgcgggc aacgtcagtt
    69001 atcaggatat gggccgccct gccgtagtgg aatgggcgca actcgcgccc ggcgtgcaca
    69061 agatccaggg cagctcccat gcgacgatcc tggtcgagat gcgcgattat ttggtcgctg
    69121 tcgaagggcc gctgtacgag gcccgcaccg cgccggtggt caagtcgatc aaagagcggt
    69181 tcccaggtaa gccgatccgt tatgcgatcc cgacccatca tcatctcgac cacgctggcg
    69241 gcattcgcgc gtttatggcc gagggggcgg cgatagtcgt gccgttcaac gcccgggcgt
    69301 tttatgagaa ggtcgcgcgc tccgcgcata cgcgcaaccc cgacagtctg gagcgcaaca
    69361 aacgggctgt gatcattgaa gcgttcggcg gcggccacag agttctcacc gacggcgagc
    69421 gccagatcgc ggtgtatccg ctcccgacgt cccatgcaga agacctggtc gtggtctatc
    69481 tgcccaagga gaaattgctt atcgaagccg accacatcag cccgcgaaac ggcgatgtga
    69541 gaccgggccc ccgggtcaag gaatttgtcc aacagttcga caagctcaat ctcgatgtcg
    69601 caacgatcgt cggcatccat ggcgatcagg cgagtcttga ggctactcga ggagcagcca
    69661 agaagtaggt ggaccatccc ggcctgggtt cgcttgggaa gcagtggagt tttccagcta
    69721 ccgaacaacg tccccatcca tcatcccatt attcaacgga aagcccctcc tgtgcctgtg
    69781 gatacttcag acgatttgat taccggagga agcttttccc atgacactca cgatctgctc
    69841 agcatcgcct cgatctacat ggcttgtgtc gggctcggat taatctttgc gcctcagcgc
    69901 tttgacgtcg gcgccgttcc caccgacccg tctgccgcgc tgatcgccta tctgcggctt
    69961 ttcggcagcc cctttctcgg gattgcggtt cttaactgga tggcgcggag cgcgggtgcc
    70021 ctccacggcg cgcaatgcca tcatcgttgg caacattgtc ggattcgccg ctatagctgc
    70081 gttggatcta tgggggattg gccagcggcg cgcggccatt aacaaaggtg ttcgcgatga
    70141 ttcatctgct tttcgccgtt gccttcatct gggtagggcg aatgagcatg tcaggcagta
    70201 gggagaagaa ataaggacgt ccttgactca gtggatcagc agtcctcagg gcattgtcgc
    70261 agatgactag ctaggcacaa cctcgccgtc tcctcatcca gatcggatga atgattagaa
    70321 ctgagcatct ggagaaggcc tcggcatcgt gcacggcatc aagcgatctt gaacagtttc
    70381 gctggtcttt tggtctttcg tttcgatcgt gttctagccg ctgcggcagt cgcgcatgcg
    70441 agcgcgcact cgacaaatcg acccaaggca gcagatagta ccgcgtcttt gcggcgaatg
    70501 aacatcgtcg gcacgagagc gtcgcgccga ggcaacggat ggatacccaa ctcggcgttt
    70561 tgcgacgcgt gttccgacac cgaacggggc atcatggtga tgcctagacc ttctctggcg
    70621 cagccaaaga cgccgtcgat cgtgccgaat tcgagttggc cgaaagaggt gacgcctgcc
    70681 tccttcaaca gccgctcaag acggcttcgg taagaacatc ccgcccgcaa gaccaggatg
    70741 tttactccct gcggagatcg gagcgcctca tagaacgagc ggaatttcgc cgacgtcacc
    70801 agcaccattt cttcggtaaa gacttgtgtc tcaacgattt catcgcgctt caccgggcca
    70861 gccacgaaag ctccttccaa tcggcacgca agcacttctt cgatgagatc gccggtcgtg
    70921 ccgatagtga gcatgagatt cacatcggga tattttcgcc ggtaggcaga taagatcgga
    70981 ggcaagcgaa cgcccgcggt ggattcaagc gagccgatct gaagattgcc gtggggcgga
    71041 ccgctctccc ccacgacgtg gactgcgtct tttagcagct gggaaatttg gacggcatag
    71101 ggcaggagcc gctcgcccgc ccgcgtcagg acgactccgc ggctgtggcg gcgaaaaagg
    71161 agggtgccga gctggtcttc gagcgcgcgg attcgtgccg tgacgtttga ttgcaccgtg
    71221 tgcagctcgt cggccgctgc ggtaatgctg cccaggcggg cgacggcttc aaacaccctg
    71281 agatcgctgc tttccatgca tgcctcttga tccgactata acacgatcaa accggcgctc
    71341 gatatcactg ctgcagatag cagtcatcac aacaaatcat tttgattttc ctccgagccg
    71401 atttacatca attaagagaa gcgacgatag cgagaatagt tctcctagaa attctgttgg
    71461 aggtgtcgca tgtcgatccg aacggctttg accaaaagat taggaatgac ttatccgatt
    71521 ttccaggcgc cgcttgccgg tggcggcgat acgccggcgc tggtagccgc tgtttgcgag
    71581 gcgggcgcgc taggtttcat cggcgcggcc tatctgaccc cagaacagat cggtgaagct
    71641 gcacataagg tacggacgca aacgtcaaga ccctttggca taaacctatt tgccccggtg
    71701 ccgatgccgg atccacctga aaatccgcgc ccgtccctcg agcgtgtcgc gccgttcttc
    71761 gccgagctgg gcttggagcc tccggttgtt cccgcgccgg cgcgcgattc attcaaccaa
    71821 caactgatag ctgctctcga aagcggcgct gccgtgttca gtttcaccgt cggcattctg
    71881 ccgaaaggtg ccatggacgc gataaagcgg cgtggcatgt ttctgattgg tacggcgaca
    71941 acggtcgagg aggcgatcgt gcttgagaag gcgggcgtcg atgcaatcgt gacccaaggg
    72001 agcgaagccg gcggtcaccg ggcaacgttc ggcgccgatt tcgatgccgg tctagttggc
    72061 acgatttcgc ttgtgcctca ggtggtcgac gccgtaaccg tgccggtgat cgcgtcgggc
    72121 ggcatcatgg acggccgcgg catcgccgca gcactggctc ttggcgcaag cgcggttcaa
    72181 ctcggcaccg cttttctgac ctgtgatgag gccggcgtgc cggaggtcta taagaaagca
    72241 atcctgggtg cgcgcgagga ccagaccagg ctgacgcgtg ctttctccgg ccgccctgcg
    72301 cgaggtattg tcaaccgctt catgttggaa gtagaaagta gcgctagcgc tgacgcaatt
    72361 ctaccgtttc cattgcagaa tcagctgacg cggcccttgc gcaacgcggc cgcgaagcaa
    72421 gggcgcgcgg aatttctgtc gctgtgggcc ggacaaggcg tccgtctggc gcagcgccaa
    72481 gcggcggcgc tacttgtgga aacgctggca acggagacag aaagggttat ccagcggctg
    72541 gcaaactgag cgctaggcgc aggcgagcac gctcatggac gtatgcgtat cgagccacag
    72601 agcgcgtctg tcgtgctcaa gaaggatgat catattcaga gcgtggagct tgaactcgtg
    72661 caacgtcccc gccatgggac cgatcttgag cgcggccgtt gtaggcgcga tcgtcggctc
    72721 tgcgctgcaa agtgcacggc cggataggtg gttgtcggcc ggatcaggac cgggtagctg
    72781 aggggtttct ccaaagtatt tccgatgcga agaagtggag ttgtcggcta gtcaggactt
    72841 gcacatctcc ttcccaaaga gcctcggctt cattctaaca aaacgacatg ccacgggggg
    72901 tattatgtta gggagcattc gtccggcgct tctgactctg gtcattgtgc ttacgattgc
    72961 gtacgcaccc gggtatgcgc ag
//
LOCUS       DASUVS010000032         6599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_227648, whole genome shotgun sequence.
ACCESSION   DASUVS010000032 DASUVS010000000
VERSION     DASUVS010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227648"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="VF182_02110"
     CDS             complement(<1..1373)
                     /locus_tag="VF182_02110"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6435895.1"
                     /translation="MNNSDVIVRMLEQAEVRWVFGVPSGPVLPLIDSLRRSSIEFILT
                     ASETSAGFMASVIGYLTGRPGICVSTVGPGATNLTTGVGCAWLDRAPVLALTCNVPSA
                     WLGRRIQMRIDHQALFKPITKRTFSFTDGKVSEILADALAIAIAEPPGPVHLDLPEDT
                     ALMSGAEKWDQPDEAKLLPDRSEALAQTISEALTKSQRPLVVTGLTFARHDSSDKLLQ
                     FLQRQKIPFVSTLHGKGFLPESHPGWAGVIGRARRTDIKRFTDQADLIIAVGYDPIEM
                     NYEEWVGETPVMHISTEAAEDDGGIAFVWNQACNLNAAVAAIAKVPPVANLWSVEDWR
                     EHRVSFDRALRPAGSQFAAHHVLDILRENLSPNAILAYDVGAHTHQIASQWRTDCPKS
                     LLATNGWSSMGYGMPAAYAAKLIFPDREVVAVVGDGGFQMTAGELSLARRLRLRVPVI
                     VLNDGWLG"
     gene            complement(1381..2181)
                     /locus_tag="VF182_02115"
     CDS             complement(1381..2181)
                     /locus_tag="VF182_02115"
                     /inference="COORDINATES: protein motif:HMM:NF009825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEX6435896.1"
                     /translation="MKTIGIVGCGAIGRALVAAAESGSLPVRIAGITSRTEQHAVEFL
                     SRFKSPPPYLSLPELIGAADLLVEAAGGSVVPALAKDVFAAGKDLMVISVGALLDHPE
                     IIESSRRTGCRLYLPSGAIAGLDGIKSACVGRIEHVTMTTRKPPDGLEGAPHLVRNHI
                     SLAGLAEEMEVFSGTTREACKGFPANVNVSAAVSLAGIGPDRTRIRILAVPGLKRNCH
                     DIEIEGEFGRLLMHIENVPSENPKTGKLTVMSIIRALQDVVDPVRIGN"
     gene            complement(2514..2942)
                     /locus_tag="VF182_02120"
     CDS             complement(2514..2942)
                     /locus_tag="VF182_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEX6435897.1"
                     /translation="MIEITSLPALNAALNSLSAVLLLTGYRFIRAGNRSAHRASMLAA
                     CASSTLFLISYLTYHFHAGSVAFKGQGLIRAVYFTILISHTILATAVVPLAIITLIRG
                     LQENFIKHRRIARWTFPIWMYVSVTGVIVYWMLYWLVPSR"
     gene            complement(2939..3217)
                     /locus_tag="VF182_02125"
     CDS             complement(2939..3217)
                     /locus_tag="VF182_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX6435898.1"
                     /translation="MATTTHTHPNYMGIFWVLLVLTVVEVGLTMIGLPKLLLGSLLVI
                     LAIWKAALVAMYFMHLKFERRTLAMIAIVPFVLCLFLILMLLPDLRPV"
     gene            complement(3234..3842)
                     /locus_tag="VF182_02130"
     CDS             complement(3234..3842)
                     /locus_tag="VF182_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HEX6435899.1"
                     /translation="MSEVAAMHPAVEPAESPLTPESWGKLGMWNFLAGDAMSFGGLIV
                     GYGILRYTSSNWPPPSEILGINLTALMTLLLIISSVTMVLALAAIQSGDKPRFKKFMG
                     FTILGGALFLGCQAYEWTHLIHETLPHHKLSFSTHLFATTFFVLTGFHGLHVTGGVIY
                     NSVVLALVSSGRWGPNNVEIAGLYWHFVDLVWILIFTFVYLL"
     gene            complement(3839..4117)
                     /locus_tag="VF182_02135"
     CDS             complement(3839..4117)
                     /locus_tag="VF182_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435900.1"
                     /translation="MKKILSAACLVVLAPALAHACAVCLTGAGDDSVSEAFNLSVLFL
                     MAAPYTVVGGIVGGLIYMNRAKAPDVQTEPDGDTTTPLATIQKESATR"
     gene            complement(4182..4844)
                     /locus_tag="VF182_02140"
     CDS             complement(4182..4844)
                     /locus_tag="VF182_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX6435901.1"
                     /translation="MARSSTARRLDEAPPAPATRLEFGGGDPPVPPVRPNAAIASNAW
                     LATVMFLGAEAMFFAGLIGAFIVFRIGSAIWPPPFQPRLPIGITGVNTIILLASAITM
                     HMARRAVRNGATGRLRRLLAVTVALGGLFLLIQGYEWARMIHFGLTVSSSIYGGLFYT
                     LIGFHAFHVAGALAWLIVVFVRATRGHFSSQKNTGLQICAMYWTFVVALWPILYGLVY
                     LY"
     gene            complement(4932..5063)
                     /locus_tag="VF182_02145"
     CDS             complement(4932..5063)
                     /locus_tag="VF182_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435902.1"
                     /translation="MTKTMNSIFSIRENQAKNRRLGFVLAAAVVLYIGAIIAFIIVY"
     gene            complement(5060..5965)
                     /gene="cyoE"
                     /locus_tag="VF182_02150"
     CDS             complement(5060..5965)
                     /gene="cyoE"
                     /locus_tag="VF182_02150"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX6435903.1"
                     /translation="MNSDTQTFELPTTRVIRRVADFCELAKPRVVLMVLITAFVGFYV
                     GSADLPQYSKLLKMLLGTALAAGGTLALNQFIERRSDALMARTRHRPIPDGRLTPAEA
                     FWFGFAGTIAGLAYLAIAVNLISAGITALITVTYLFLYTPMKRKSPLCLIIGAVPGAL
                     PPVIGWVAARGTLDIDAWVLFAILFLWQVPHTLAIAWLYREDYAKAGIQFLPVIEPGG
                     SSTNRQIISHCAALLAVSLLPTLLGLAGAVYFVVAFVLGIGFLVSGVRLAMESNLVGA
                     RRLLYASLIYLPVLLLMMALDRVPL"
     gene            complement(5962..>6599)
                     /locus_tag="VF182_02155"
     CDS             complement(5962..>6599)
                     /locus_tag="VF182_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435904.1"
                     /translation="WLGMLALALVVIQGVLGGLRVTLVEHSLAIVHASLAQAFFALTV
                     SLALFTSPEWREKVAEPPLADYGRLRRLAAVTTGLIYFQVFFGAVLRHTGLRLDAHLL
                     FAVLVTLHVVLLAVRIFKEHTGDGRLTRPALLLSAMLLLQLFLGTVSYLSKFTTLLSI
                     PIDATVFLTTTHLAVGALMLVTSLIVTLQLYRYPTLSNSAGQEVFTEQYSL"
BASE COUNT         1690 a         1907 c         1771 g         1231 t
ORIGIN      
        1 cctagccagc cgtcgttcag aacgatcacc ggcacccgca agcgcaagcg gcgagcaagc
       61 gatagttcgc ccgcggtcat ctgaaagccg ccgtcgccta ccaccgcaac aacttctcgg
      121 tcagggaata tcagcttggc cgcgtacgcc gcgggcatgc cgtagcccat ggaggaccag
      181 ccattggtcg caagcaacga cttgggacaa tcggtgcgcc attgactggc gatctgatgc
      241 gtgtgcgcgc caacgtcgta ggcgagaatg gcattcggtg agaggttctc acgcaagata
      301 tccaacacat gatgcgcggc aaactggctg ccggcgggac gcagcgcccg atcgaacgag
      361 acgcgatgct cgcgccaatc ctcgacagac cacaggttgg caaccggcgg aacctttgcg
      421 atcgccgcaa ctgccgcatt gagattacaa gcctgattcc agacaaacgc gataccgcca
      481 tcatcctcag ctgcttcggt actgatgtgc atgaccggag tttcgccaac ccattcctcg
      541 taattcattt cgatcggatc gtaacccact gcgatgatca ggtcggcctg atcagtgaaa
      601 cgtttgatgt cggttcggcg cgcgcgcccg atcacgcccg cccaaccggg atgactttcc
      661 gggagaaagc ctttgccatg aagcgtcgac acaaacggaa tcttttgtcg ttggagaaac
      721 tgcaaaagct tatccgaact gtcatggcga gcgaaagtaa gtccggtgac caccagcggc
      781 cgctgcgatt tagtgagcgc ctctgaaatc gtttgagcga gcgcttcgga acgatccgga
      841 aggagtttcg cttcatccgg ttgatcccac ttttcggctc cactcatcag agcggtgtcc
      901 tcgggcaaat ccagatgaac cggtccgggc ggttcggcga tggcaatcgc cagagcatcg
      961 gcgagtattt cgctgacctt gccgtctgta aaagaaaaag tcctcttggt gatgggcttg
     1021 aataaggctt gatgatcgat gcgcatctga atgcggcggc ccagccaggc gctcggaaca
     1081 ttgcatgtca acgccagcac cggcgcacgg tcgagccagg cgcaaccaac gccggtcgtg
     1141 agattggtgg ctccggggcc cacggtggaa acacagatgc ccgggcggcc ggtcagatat
     1201 ccaatcacgg atgccatgaa tcctgccgac gtttcgctcg ccgtcaggat gaattcgatg
     1261 gaacttcgcc gcaacgagtc gatcagcgga agaaccggac cgctgggcac tccgaaaacc
     1321 cagcgcacct ccgcttgctc gagcatccgg acgatgacat cgctattgtt catgggggat
     1381 ctaatttcca atgcgcacgg gatcgacgac gtcctgcagc gcgcgaatga tggacatcac
     1441 ggttaatttt ccggttttcg gattctcgct gggcacattt tcgatgtgca tcagcaagcg
     1501 gccaaactcg ccttcgattt cgatatcatg gcaattccgc ttcaatccgg ggacggcaag
     1561 gatgcggatt cgcgttcgat caggaccgat gccggccagg ctcaccgccg ccgaaacgtt
     1621 cacgttcgcg gggaagcctt tgcacgcctc gcgcgtcgtg ccggagaaca cttccatctc
     1681 ttcagcaagt ccggcaagag agatgtggtt tcgcacaaga tgcggcgcgc cttcaagccc
     1741 gtcaggcggc ttgcgcgtgg tcatcgtgac gtgctcgatc cgccccacac aagcggattt
     1801 gatcccgtcc agaccggcga tagctccgga ggggagatac agccggcaac cggtgcggcg
     1861 ggagctttcg atgatctccg gatgatcgag tagagcgcca acactgatca ccatgagatc
     1921 cttaccagcg gcaaatacat ccttggctag tgcaggcacg accgatccgc ctgcggcttc
     1981 gacgagcaaa tcggcagctc cgatcaactc gggcaaggac aggtacggtg gagggctttt
     2041 gaacctcgac agaaactcaa cggcatgctg ctccgttcgg ctggtaatcc cggcaatgcg
     2101 cacaggtaaa ctaccacttt ccgcggccgc aaccagcgcg cgaccaatgg cgccgcaacc
     2161 gacaattcca atagttttca cgaaaccctc cccaatgtcg gaatcaagga tcttgatgcg
     2221 ccggctcacc tgaaatgcgc atgcggattt ctgatgaaca ggactcgtcg tcccataata
     2281 atcctgcgca aagccgatca cgttcaatct gagcagccgg taagcttccg ttgctacgca
     2341 acggccctca atgttagggc gatctcagcc tagaaatgtg tcccggccca cccatcaaag
     2401 agagatgaac tgcttggttt aagttcgctt ctcaggcgtt gccggaatca tctctcgact
     2461 ctcatctcta ctgcggatgc cgcgctgaat acaaccgccg cctgagcagg gcgtcagcgt
     2521 gacggcacca gccaatagag catccagtac acgattacgc ctgtcacaga aacatacatc
     2581 catatgggaa aggtccagcg ggcgatccga cgatgcttga tgaagttttc ttgcaggcct
     2641 cgaatcaagg tgatgatcgc cagcggcaca accgcggtgg ccaggatggt atgcgaaatc
     2701 aagatcgtaa agtacacggc ccggatcaag ccttgtcctt tgaaagccac tgaaccggca
     2761 tggaaatggt aagtcaaata ggaaatcaga aacagggtcg aggaggcgca cgctgcgagc
     2821 atgctggcgc ggtgcgccga tcggtttcca gctctgataa atcgatagcc cgttagcaac
     2881 agaacggcac tgagtgaatt tagcgcggcg ttaagcgccg gcaacgaagt tatttcaatc
     2941 aaacgggacg gagatcgggc agcagcatca aaatcagaaa caaacagagg acaaacggaa
     3001 cgattgcgat cattgcgaga gttcttcgtt cgaacttcag atgcatgaaa tacatcgcca
     3061 ccagcgcggc tttccatatt gccaggatca cgagcagcga acccaacaaa agcttcggca
     3121 gtcctatcat ggtaagccct acctccacca ccgtgagcac cagaagcacc caaaaaattc
     3181 ccatgtaatt gggatgcgta tgcgttgtcg tagccatgtg tttcccctag aacttacagc
     3241 agatagacaa acgtgaagat tagaatccac accaggtcga caaagtgcca gtacagaccg
     3301 gcaatctcga cgttattggg gccccatcga ccgctgctca ccaacgcaag caccacggag
     3361 ttgtaaatga cgccgccggt tacatgcagc ccgtggaaac cggttagaac gaaaaatgtc
     3421 gtggcaaata agtgagtcga aaaagaaagt ttatggtgag gcagtgtttc atggatcaag
     3481 tgcgtccact cataggcttg acagccgaga aagagcgcgc cgccgagaat tgtaaacccc
     3541 ataaactttt tgaatctggg cttgtcgccg gactggatcg cggccaacgc caagaccatc
     3601 gtcacactgc tgataatcag cagcaaagtc atcagcgctg tcaaattaat gcccagaatt
     3661 tccgatggcg gcggccaatt cgaactcgta taacggagga taccgtagcc gacgatcaac
     3721 ccaccgaaag acatcgcatc gccggctaga aaattccaca ttccgagttt gccccagctc
     3781 tcgggagtca gaggcgactc agcgggctca accgcgggat gcatcgcagc aacttcactc
     3841 atcttgttgc actctccttc tgaatcgttg ctaaaggtgt tgtggtgtcg ccatcgggtt
     3901 cggtttgaac atccggagcc ttggctcggt tcatataaat caggccaccg acaatcccgc
     3961 cgacaacggt atagggcgcc gccatcaaga acaacacgct caagttgaag gcttccgaga
     4021 ccgagtcatc acctgcgccg gtcaggcaaa ctgcgcaagc atgcgccagc gcaggggcca
     4081 aaaccaccag gcaagcagcc gataaaattt ttttcatcaa tacactcgtc tatttaacag
     4141 ccgaagctaa cggatcattt cttcgctgtc aacaaaagcg atcaatacaa atacaccaag
     4201 ccataaagaa tcggccacag ggcgacaaca aacgtccaat acatggcgca gatttgcaaa
     4261 ccggtgtttt tttgactaga gaagtgaccc cgtgtggccc gcacaaacac cacaattaac
     4321 caagccaatg cgccggccac atgaaaggcg tgaaatccta tcaaggtata aaataaaccg
     4381 ccgtagatgc tgctcgaaac cgtcaacccg aagtgaatca tgcgagccca ctcgtatccc
     4441 tggatcagca ggaaaagacc accgagcgcg acagtcactg ccagcagacg ccttaggcga
     4501 ccggtggctc cgttacgaac cgctcgacgc gccatatgca tggttatcgc gctggcaagc
     4561 aggatgatcg tattcacacc ggtgataccg atcggcagcc tgggttgaaa tggtggcggc
     4621 catatcgcac tgccgattcg aaacacgatg aaagcgccga tgagcccggc gaaaaacatc
     4681 gcctcagcgc caagaaacat gacggttgcc agccaggcat tgctggcaat tgccgcattc
     4741 ggccgcaccg gaggaaccgg cggatcaccg ccgccaaact cgagccgggt tgccggagca
     4801 ggcggagctt catcgagccg tcgtgcggtc gaacttcgtg ccataagcgt cgccaacggt
     4861 ctttctcagt aagctatcac aaccggaaga gttgattcct gtttccaacc agctcctggc
     4921 gcttaccttt ctcaatagac aatgatgaag gcgataatcg cgccgatata gagaaccact
     4981 gccgccgcca agacaaaccc gagacgccgg ttcttagcct gattttcacg aattgaaaaa
     5041 atactattca tggtcttagt catagcggca cccggtccaa cgccatcatc aacaaaagca
     5101 ccggcagata aatcaatgag gcgtagagta gccggcgagc gcccaccaaa ttcgattcca
     5161 ttgccaaacg gaccccacta acaagaaatc caatgcccag aacgaaagca accacaaaat
     5221 aaactgcgcc cgccagacct aaaagggtcg gcaatagact gacggccagc aaggcggcgc
     5281 agtgagaaat gatctgccga ttagtgctgg agcctccagg ctcaatgacc ggcaaaaatt
     5341 gaatccctgc cttggcatag tcttcacggt acagccaagc aatcgcaagt gtatggggca
     5401 cctgccagag aaatagaatc gcaaacaata cccaggcatc gatatccaga gtgcctcgcg
     5461 cggcgaccca gccgatcacc gggggcagtg cgccgggcac cgcgccgata atcaagcaca
     5521 gcgggctttt ccgcttcatc ggtgtataca agaacagata agtaaccgtg atcagcgcgg
     5581 taattcctgc gctgatgaga ttgacggcta tggccaggta ggcaagcccc gcaatcgttc
     5641 ccgcgaagcc gaaccaaaaa gcctctgccg gcgtgagccg cccgtccgga atcggtcgat
     5701 ggcgcgtccg ggccatcaaa gcgtcgcttc gccgctcgat aaactggttc agcgccaatg
     5761 taccacccgc cgccagcgcg gtgccgagca gcatcttaag tagcttggag tactgcggca
     5821 gatcagccga gccgacataa aatccgacaa acgccgtgat cagcaccatc agcacaaccc
     5881 gcggcttagc aagctcgcag aaatcggcga cccggcgaat cacacgcgtg gttggcaact
     5941 cgaatgtttg ggtgtccgag ttcatagtga atattgctct gtaaaaactt cttgcccggc
     6001 ggagttcgat agagtcggat accggtaaag ttgcaacgtc actatcagac ttgtcacgag
     6061 catcagagcg ccaacggcta aatgcgtcgt ggtaagaaac accgtcgcat cgatgggtat
     6121 gcttagaaga gtggtaaatt tgctcagata cgaaacggta cccaaaaata attgcaacaa
     6181 caacattgcg ctgagcagga gcgcggggcg ggtaagccgg ccgtcgcccg tgtgttcttt
     6241 gaaaatccgt acggcaagaa gcacaacgtg caacgttacc agcacggcaa acaacaaatg
     6301 ggcatcgagg cgcaggccgg tatgccgcag cacggcgccg aagaacacct ggaagtagat
     6361 gagtccggtt gtcacggcag caagccggcg caagcgtccg taatcggcta gcggcggttc
     6421 ggcaaccttc tcgcgccatt ctggagaggt aaacagagcc aggctgaccg taagcgcgaa
     6481 gaaagcttga gccaatgaag cgtgaacaat cgccagggag tgctctacca acgtcacccg
     6541 taaaccgccg agaacgcctt gtatcacgac tagcgccagc gcgagcatcc ccagccaac
//
LOCUS       DASUVS010000033         5444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1319671, whole genome shotgun sequence.
ACCESSION   DASUVS010000033 DASUVS010000000
VERSION     DASUVS010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319671"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(408..1559)
                     /locus_tag="VF182_02160"
     CDS             complement(408..1559)
                     /locus_tag="VF182_02160"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6435905.1"
                     /translation="MADYKLISADSHVNEVAATWERVRRQYGERAPELVWNPSAKEYG
                     PYLKIRDWETSLEGKDRESCAMEFVGMAIGGLGVGSVVGRSSERASEFLKNFRFEDWP
                     GPWEPNARLKDMDRDGVEVDILYASHLRHVYGLSIKDEPFFRAIAQSYNDWLMEYCSV
                     APNRLVGLPVLSILNIDGAVEDLAFYAKQGAKGFMIASSTPIGMTYSERKFDRLWASA
                     QDADVPLCLHTTTGAWKKAKFHHPRIRTFIRGEGEIQTSLLEMIYGGVFDRFPRLKIV
                     AAEWDIGWVAHMVAKLKDHDPKTGLKLAPADYFRRNIWFTFENDRAGVLTTPLYGADR
                     FLWASDYPHGATTWPDSQAIVDQQFEGISEETKRKVTRQNAIDLYKLSL"
     gene            complement(1586..2764)
                     /locus_tag="VF182_02165"
     CDS             complement(1586..2764)
                     /locus_tag="VF182_02165"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6435906.1"
                     /translation="MWKSFKVIDADAHMHEPKDMWERYVEAKYKDRVPKVAYMDGTFM
                     VYEPDGKIIPKGQIESRPPQTAWNALEEKYGEAYHKWWSPEIRLKDMDRHGWDIQVLL
                     PTGSNGNFGPRAALKDSEIGAALCRAYNNWAYDYCSASPQRLKFIAVIISTNIDEMLA
                     EGRRAVEKLGAVSLRNILLPKGRWLHEPEYDALWSLASDLDVPVAVHGETRNQRFQPF
                     RELVIEEENTSQKTPHHDAMTLRGLNHAMGFPYDNMATLGQFIFTGILERFPKLRLAI
                     LESNAGWAPFWLGRMDVHTHGRHGVMGKPEHLSMLPSEYFKRQCTVACDSDEAALKYV
                     ADFLNGENIVWNTDYPHADGMEPARALPDFDAHPISQEHKRKILWDNAVKLYGRRLIS
                     "
     gene            complement(2918..3823)
                     /locus_tag="VF182_02170"
     CDS             complement(2918..3823)
                     /locus_tag="VF182_02170"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX6435907.1"
                     /translation="MTLHQLKIFQVVSRHRNITKASKELRISQPSVFKQVKLLEESYR
                     TKLYKKIGRGIELTEQGQLFEREVEEILRRIDSLEKNFGVRSAAPAAGRLMIGASHGP
                     SASLIPSLLANFKKMHPLVQVTLRTKDSLGIERLVAESGGIELGVVTNLSRMADSARL
                     HIEPCRAEEIVFFVSAKHVPVKRKKLTLAELARLPLIVKQRSQSKTLDLLKQIEQQGF
                     ELNVFMECESAEAVKLAVMKNLGVGVLYRDHLKAEIRRGDLKVLSISDLKPTAIESFI
                     IYNRNRPISPNAQEFLELLRKSQHH"
     gene            4052..5104
                     /locus_tag="VF182_02175"
     CDS             4052..5104
                     /locus_tag="VF182_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6435908.1"
                     /translation="MTLIAIRIIAAVAMWFLPMPTRAAEIGSIALYRGADRQARLEKA
                     AHAEGSIVWYTSIALGESSRYVESFEKRYPSIKVNLVRSNSSRMAQRYGAEYQAGTYL
                     ADVLDTGDTTIEFFRRKGMLQPYYSPLAEKFDKRFVQNENFWVANRATMTVLGYNTRL
                     VKPAEAPARYEDVLDPKWKDKISLEREDSQWFMALFEHWGEQKAKAFFHRLRGQNPRI
                     RTGHTLQAQLIAAGEDALSPDTHSGGIAAARRAGAPVEWLNLEPVVAANNVAALARNA
                     PHPNAAMLFLDFMLSKDGAQQILRDVNRIPTHPEVLPNPPRLREGFNFIVIDPAKYND
                     GIERYGKLWREWVLQE"
BASE COUNT         1197 a         1524 c         1430 g         1293 t
ORIGIN      
        1 tgcaaataac ctacgacctg agtaacgctg agaaggcgct gccggcaagg gtgagaaaaa
       61 gtatcgaaga gagcgggagc ctgctgccat gacgatgccc ctttgattcc cggcagtgcg
      121 tcgagcccgt tccaatcggt atcgaagttc ttaccgcgac cttcacagct tcggatcagt
      181 tgctcctacg gcgcggcagt gtgagctacc aatctctcgt gagtttggcc agcgcgaaag
      241 gcgagctaaa actaagtgac gcgaccggaa atgtcaaaag taagatttga cgcctgtcgg
      301 tccccccttg acctagcttt tggaccgagc tgagcgccca aagagtgccc cagtgatgtc
      361 atgggggaca gcgctgcgcg gggagatcaa agcagacgcg gcttgtatca gagcgataat
      421 ttgtacaagt cgatggcgtt ctgccgcgtt actttacgct tcgtctcttc tgagatgccc
      481 tcgaattgtt gatccacaat cgcttgtgaa tccggccaag ttgtagcgcc gtgcggatag
      541 tcgctcgccc acaaaaaccg atcggcgcca tagagaggcg tggtcagaac cccggcccgg
      601 tcgttctcaa acgtgaacca gatgtttctg cgaaagtaat ccgcgggcgc cagtttcagt
      661 ccagttttcg gatcgtggtc cttgagtttg gcgaccatgt gagcgaccca gccgatgtcc
      721 cattcggcag cgacaatttt caagcgcggg aaccgatcga aaacaccgcc gtagatcatc
      781 tcgagaagcg aggtctgaat ttcaccttcg ccgcgaatga aggtgcgaat tctcggatga
      841 tgaaacttcg ctttcttcca cgcgcccgtg gttgtatgaa gacagagcgg cacatcggca
      901 tcctgggccg aggcccacaa ccgatcaaac tttctttcac tgtaagtcat tccgatgggc
      961 gttgacgatg cgatcatgaa acccttcgct ccttgcttcg cgtaaaacgc caggtcctca
     1021 accgcaccgt cgatgttcag aatggaaagt accggcagac cgacgagccg gttcggcgcc
     1081 acgctgcaat attccatcag ccagtcgtta taggattgcg cgatggcgcg gaaaaacggc
     1141 tcatctttta tgctcaaacc gtaaacgtgg cgcaggtgac tcgcatacaa aatatcgact
     1201 tcaacaccgt cccggtccat atctttgagt cgcgcgttcg gttcccatgg ccccggccaa
     1261 tcttcgaagc gaaagttctt gagaaattcg cttgcgcgct ccgaacttcg cccaacgacg
     1321 ctgccaacgc ctagaccgcc aatcgccatc ccgacgaact ccatcgcgca ggactcacgg
     1381 tctttaccct cgaggcttgt ttcccaatcg cgaatcttta aatacgggcc gtattctttt
     1441 gcgctcgggt tccacacgag ctcgggggcc cgttcgccgt attgccggcg cacccgctcc
     1501 caagtcgcag cgacttcgtt gacgtgggaa tctgcggaga tcagtttgta atccgccatt
     1561 tggcagccct cccgattcaa tcaagttagc ttatcaagcg ccgtccgtag agcttcaccg
     1621 cattgtccca gagaatcttg cgtttgtgtt cttgcgaaat tggatgtgcg tcgaaatccg
     1681 gcagcgccct cgctggttcc attccatccg cgtgcggata gtcggtattc catacgatgt
     1741 tttcgccgtt caaaaagtcc gcgacgtact tgagcgccgc ttcgtcgcta tcgcaagcca
     1801 ccgtgcattg ccgcttgaaa tattcgctcg gcagcatcga cagatgctca ggttttccca
     1861 ttacgccgtg gcgaccgtgc gtgtggacat ccatacgacc gagccaaaag ggtgcccagc
     1921 cggcattgga ttcaaggatc gccagcctga gcttgggaaa gcgctctaga ataccggtga
     1981 agataaactg accaagtgtt gccatgttgt cataaggaaa tcccatcgca tgattgagtc
     2041 cgcgcagtgt catcgcatcg tggtgcggcg tcttttgact agtgttttcc tcttcgatga
     2101 ctaactcgcg aaacggctga aaccgttggt tgcgagtttc gccatgaacc gcgaccggca
     2161 cgtccaagtc cgatgcgagc gaccacagag catcgtactc gggctcatgc agccacctgc
     2221 ctttgggcaa aagaatgttt cttaacgaaa ccgctccgag cttctcgacc gcccttcggc
     2281 cctctgcgag catctcatcg atattggttg agatgatgac cgcaatgaac ttgagccgtt
     2341 gcggggaggc gctgcaataa tcgtaagccc agttattgta ggcgcggcac aacgctgcgc
     2401 caatttcact gtccttcagc gccgcgcgcg ggccgaagtt gccattgctt cccgtgggta
     2461 ggagaacctg aatatcccac ccgtggcgat ccatgtcttt aagacgaatc tcgggcgacc
     2521 accatttgtg ataggcttcg ccgtatttct cttccaatgc attccatgcc gtctgcggag
     2581 ggcggctttc aatttggccc ttgggaatga tcttcccatc gggctcataa accataaatg
     2641 tgccgtccat gtacgctact ttgggaaccc ggtctttgta tttggcctcg acataacgct
     2701 cccacatgtc cttgggttcg tgcatatgcg cgtcggcatc gataaccttg aatgatttcc
     2761 acatagggca tctccttcgc tagttctcgc ctgcaaaaat agatgcgggc accggccctg
     2821 tccaatactt ttttgtcagc agaaactata gacgttcgtc tattttggcg gtcggatgtg
     2881 gcttgacccg atgcagttga agcgagacag gttaccgcta atggtgttgc gattttctca
     2941 gcagctcaag aaattcctgg gcattcgggg agatcggtcg gtttcggttg tagatgatga
     3001 acgattcgat ggcggtcggt ttaagatcgg aaatgctcag gactttgaga tcgcctcttc
     3061 taatctccgc tttcaagtga tcgcggtaaa gcactcccac gcccagattt ttcatgacgg
     3121 cgagtttaac ggcctccgca gactcacatt ccataaaaac gtttagctca aacccctgct
     3181 gctcgatctg cttgagcagg tcgagagtct tggactggct tctctgcttg acgatcaagg
     3241 gcagtcgcgc caattcggcc aacgtcaact tttttctttt gaccggcaca tgcttggcgg
     3301 agacaaaaaa cacaatctcc tcggctcggc agggctcaat gtgaagccgg gccgaatccg
     3361 ccattcgaga gagattggtt accactccaa gctcgatccc gccggactcg gcaacaaggc
     3421 gctcgatgcc gagactgtct ttggttctaa gcgttacctg aactagcgga tgcatctttt
     3481 taaaatttgc cagtagcgaa ggtatcaaag aggccgatgg tccatgcgag gcgccgatca
     3541 tcaaccggcc tgccgcaggc gccgcggagc gtactccgaa gttcttttcc aggctgtcaa
     3601 tgcggcggag aatctcttcg acttcgcgct cgaaaagctg accctgttcg gtcagctcta
     3661 tgcctcgacc gatctttttg tagagtttgg tcctgtagga ctcctccagc aactttactt
     3721 gtttgaacac ggagggttgg ctgatacgca gctctttcga agctttcgtg atgttgcggt
     3781 gccgggacac cacctggaaa atttttagtt gatgcaaggt catggcgttc gcttctcgta
     3841 gtaaagcgcg cgtttacgcg atcattatca gccggtgcct tcccgatcaa tatacaaaca
     3901 tctatagcta cgtcatgaca aaaaagtagt ggacgcaaga cagctcctag ccttatctta
     3961 cgccacatcc acaaagcaca tcggttttga catcgtcaat cgcgtggccg cgcgcgcaag
     4021 cgaaatccag agagtgagga cgcaaacgat catgacgctc atcgccatca gaattatcgc
     4081 tgccgtcgcc atgtggtttt tacccatgcc tacacgcgcg gcagagatcg gatcgattgc
     4141 tctctatcgc ggcgccgatc ggcaggccag actggaaaag gcggcgcacg ctgaagggtc
     4201 gatcgtttgg tacacttcca tcgcattggg cgaatcatcg cgctatgtcg aatcgttcga
     4261 gaagcgctat ccatctatca aggtcaatct cgttcgctcg aattcatcca gaatggcaca
     4321 gcgctacggc gccgaatatc aggctgggac ttacctggcg gacgttctcg acaccggcga
     4381 taccaccatt gaattttttc gccgcaaggg aatgctccag ccgtactact cgccgttggc
     4441 agagaagttc gataagcgat ttgtgcaaaa cgaaaatttc tgggttgcga accgggcgac
     4501 aatgacggtg ctcggctaca acacgcgtct cgtcaaacct gcggaagcac cggctcgcta
     4561 tgaagacgtg ctcgatccca agtggaagga caagatttca ttagagcgcg aagattccca
     4621 atggttcatg gcgctgtttg aacattgggg cgaacagaag gccaaggcgt tctttcacag
     4681 gctgcgcgga cagaatccac gaattcgcac tggccacacg ctccaggcgc agcttatagc
     4741 ggccggtgaa gacgctcttt cccccgacac acacagcggc ggaattgccg ccgcgcgccg
     4801 tgctggcgcg ccggtcgagt ggctgaatct cgagcccgtc gtggcggcca acaacgtcgc
     4861 cgcgttggcg agaaacgcgc cccatcccaa tgcggcgatg ctttttcttg atttcatgtt
     4921 gagcaaagac ggcgctcagc agattcttcg cgacgtgaat cgcattccca cgcatccaga
     4981 ggttttacct aatcctcctc gattgcgtga gggattcaat tttatcgtga tcgatcccgc
     5041 gaaatacaac gacggcattg aacgttacgg aaaactgtgg cgcgagtggg tgctgcaaga
     5101 atagatcgtt cctgcaccgc agtggatttt tgcctagcgt cagcccaaag agattgacac
     5161 tactcctcga cgacttgttg caccgccagc cgattgaact actggaccac cgttccatct
     5221 gctttggatc gatcctgtgg ccatccacat ctgctatgga tcatcaaggt tagttttcca
     5281 tttcggcata tagaaatgca gcgatatccg tgctggcgag cgtcgatgcc gctgcacggc
     5341 tttaatttct tcacaggttg tttcaacgca atgagacatg aggggtcagg tctttactat
     5401 tgctcttcgg tcgcattctt aatcaggcga ccactgccgg ttga
//
LOCUS       DASUVS010000034         7679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_9072, whole genome shotgun sequence.
ACCESSION   DASUVS010000034 DASUVS010000000
VERSION     DASUVS010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7679
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9072"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /gene="pgk"
                     /locus_tag="VF182_02180"
     CDS             complement(<1..363)
                     /gene="pgk"
                     /locus_tag="VF182_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX6435909.1"
                     /translation="MICGIDQIDISGKKVFLRVDFNVPLANGKITEPHRIESAVPTIR
                     LIMEKSKKVIIASHLGRPSGRKDPKFSLAPVRAYLEQSLGQTVVLAPDSIGSEVEKLA
                     LDPSSRVILLENLRFHAEE"
     gene            516..1208
                     /gene="tolQ"
                     /locus_tag="VF182_02185"
     CDS             516..1208
                     /gene="tolQ"
                     /locus_tag="VF182_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX6435910.1"
                     /translation="MPQSHGILDLVRGSGIVVQTILYLLVLFSVVGWGIIAFKFRQMR
                     RAKKESQKFIEIFWERRNLSSIHDASRELKSSPVGQVFRSGYEELVRVSRSKKDSPAG
                     DNLTTELGGVDNVSRAMKRATSVEITKLEKHCTFLATTASAAPFIGLFGTVWGIMNAF
                     MGLSTTHSSSIQAVAPGIAEALIATAVGLAAAIPALMAYNYFVQQIKVLAVEMDNFSH
                     EFLNIAERHFFK"
     gene            1223..1645
                     /gene="tolR"
                     /locus_tag="VF182_02190"
     CDS             1223..1645
                     /gene="tolR"
                     /locus_tag="VF182_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEX6435911.1"
                     /translation="MAMDTSSSRDSTTISQINVTPLVDVMLVLLVIFMVTAPIIQQGV
                     QVNLPQAKAAAIPGTDDLLVVSIAKNGRIYLNDNVMTLDELGAKMRAIRTNFADRQVY
                     VRADQDVRYGLVMQTIAELKQAGIERVGMVTRPTLEDG"
     gene            1648..2676
                     /gene="tolA"
                     /locus_tag="VF182_02195"
     CDS             1648..2676
                     /gene="tolA"
                     /locus_tag="VF182_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HEX6435912.1"
                     /translation="MAGESNSTSTASGHEPLTDDRLSNWLLFSFLLHGAILIALIFSP
                     YFPSNTRPATPVYTVDLVGGEKIGGNRSGTELNPAASPKDFPKKVRSEPPAPAPEIKQ
                     SKPKVQEKSEKAVKAETPKPEKSEKLEKPEKVVKPTEKAPAHEDQVALKKTPVKKEEP
                     KKEELKKPEPRSEPAKESKEIKNTAAPEGGAMDKVRERLIQSALDRVKSRAESTQKAA
                     GTSKAGEVLSVGPGEGDGATGLGQGGRGGGTAVRGAEFFMYQGKIVNTIKENWAWPGQ
                     KGSLKALVRFSIKDNGEITGLKIAEPSGDASFDESILRALRKSSPLPPPPESYRKDFA
                     QVEMNFQP"
     gene            2690..3997
                     /gene="tolB"
                     /locus_tag="VF182_02200"
     CDS             2690..3997
                     /gene="tolB"
                     /locus_tag="VF182_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEX6435913.1"
                     /translation="MKQKITSAVIFYLLMASASYAQVRLDVIGPGMPKYPIAISPLKN
                     AGNGADPGRISEGVADAIVYDLDLSGWFRVLDRATYIEHPQRSGDTLETIDFKDWSTI
                     GAVGLVKGNFSVQGDEVTVELRLFDVTQARQTIGKRYTGRARDFRRIAHKFADEIINQ
                     FTGVPGIFNTRIAYTSTGGGRFKEIHLAHLDGSEKYQVTNNRTINLSPSWTPDGKSIL
                     YTSYKERNPSLYLFELFSGKDLKFSSRTGLNLGGKWSPDGQHVAVSLERGGNTDIYLL
                     EKSGDILRRLTEDTSIDVSPSWSPDGRQLVFVSDRSGSPQLYIMDVASGKSRRLTYGG
                     SYNTSPDWSPAGDRIAFISRVGGRFSIFSIPVGGGEPTRLTTNAFDNEDPAWSPDGRF
                     IVFSSNRAGRYHLYVMQATGENQRRLTGSGGDDTKPSWSPRLE"
     gene            4049..4654
                     /gene="pal"
                     /locus_tag="VF182_02205"
     CDS             4049..4654
                     /gene="pal"
                     /locus_tag="VF182_02205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX6435914.1"
                     /translation="MGDFRMLCYGIVAAFILLSGGCAPSAVKPDDGSVGSTGSSSVTP
                     SGSDGQSAGDGRAGATKESNVPSTSSSLDQLKAGKPPVTSASSPLKDVLFEFDSYDLR
                     GNARDVLRSNADWLKSNPSARIEVEGHCDERGTSEYNLALGAKRSQTAKDYLVSLGIS
                     PERVSTISYGEEIPVCTEHNENCWRQNRRARFVVVPSRPAS"
     gene            4691..5527
                     /gene="ybgF"
                     /locus_tag="VF182_02210"
     CDS             4691..5527
                     /gene="ybgF"
                     /locus_tag="VF182_02210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEX6435915.1"
                     /translation="MLIHRIALIAFFSVGVTACAQPQQIELLEREQRRIRSDHATIHN
                     DVESMRSSLADTRANMQQLQREFSALKERIEETRYQVGRQIGQSSRDGDQRVKDLEAR
                     VAKLTEAFKEQDGQLKAREEELRELQKNTRQQQPVAGDTSAEKSGISAAENETVRSDY
                     EVAWRALEKKDYRLAITRFKEFLQKHPKSKLANQAQYWIGESHYALREFDQAILEFDA
                     VRSRYPQADKVPAALLKQGFAFAELGEKVNARLILQEVVEKYAQSPEAVQAKQKLRSL
                     ES"
     gene            5623..6567
                     /locus_tag="VF182_02215"
     CDS             5623..6567
                     /locus_tag="VF182_02215"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX6435916.1"
                     /translation="MEVFRDIDDFHRSDLKTVVTMGNFDGIHLGHQALVSNAVTEARR
                     WRTECMVFTFEPHPLKLLAPDRAPQMIVNHQDKMDMLEALGVDAVVVQTFDRDFANIE
                     AKDFVRRYLVEHLNLSKIWLGRDLRFGRARKGDAEDLIRWGRELGFGVGIVEPILIQG
                     KRISSSRIRQLIQEGRVDEVRPMLGRYHFISGRVVRGQRRGRNIGFPTANLSAATELL
                     PQDGIYATILQFQGRRRLSVSSVGHNPTFGEGLHTIEAYIFDFAQDIYGEPVKLSFVQ
                     RIREERKFAQIEDLVTQIHQDVLAARAIFDQMNLGYEN"
     gene            6567..7601
                     /locus_tag="VF182_02220"
     CDS             6567..7601
                     /locus_tag="VF182_02220"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX6435917.1"
                     /translation="MDSSIAVQQTMVGEKASGMRLDLFLRQHLINEAERLGLTRSRMQ
                     KLIEDGTITVNGAPTRSSRRLRPSDSVEVRTIPNNIETKLRPEDVVLDVIYEDRDCIV
                     INKAPGMVVHPASGRISGTLVNALLHHCPELKEIGADGRPGIVHRLDKDTSGVMVVAK
                     NHRSLQILAQQFKDRTVQKEYLALVWGKFNSDSGIIDRPIGRHRYQRKKMSCRFADSR
                     KREALTEWNVERCLELRADSRSTRWVSLCRLRPKTGRTHQIRVHLAELGHPVIGDKVY
                     GRLAKPDVKNSTTLALLYDFPRQALHAEKLEITHPTTGYRIQFKAALYNDIYEVINEA
                     EKWRRPIDRG"
BASE COUNT         1962 a         1847 c         2073 g         1797 t
ORIGIN      
        1 ctcctcggca tggaagcgca gattttcaag tagaataact cgcgagcttg gatcgagggc
       61 gagtttctca acctctgagc caatagaatc gggtgccaga actactgttt gtcccagact
      121 ctgttccaga tacgctcgca caggtgcgag gctgaatttc ggatcctttc taccgcttgg
      181 acggcccaga tgcgaggcga tgattacttt cttcgacttc tccatgatca atcggatggt
      241 cggcaccgca ctttcgatcc gatgtggctc ggtgattttg ccgttggcga gaggcacatt
      301 aaaatcgacg cgtaagaata ctttctttcc tgagatgtcg atttgatcaa tgccgcaaat
      361 cataatgatg cccctgattg accgtttaat gtcgagaaat ccgtatttac taattgacta
      421 ataacagagg ttataatatt ttttcaaata gcatttcaaa atccaacatg ttttctttct
      481 ggtcgctcca tcttttagcc caaaatggct cgtttgtgcc gcaatcgcat gggattctcg
      541 atctcgtccg ggggtcaggg attgtcgttc aaacgattct ttacctgctg gttctattct
      601 cggttgtcgg gtggggtatt atcgcattca agttccggca gatgcgcaga gctaagaaag
      661 agtcacagaa attcatcgaa attttctggg aaagacgcaa cttgtcctca atacacgacg
      721 ccagtcgcga attgaaatcc agtcctgtag gccaggtctt tcgctcaggt tatgaagaac
      781 tcgtccgtgt atcacgctca aagaaggatt cccctgccgg cgataacttg acgaccgagt
      841 tgggcggcgt tgataatgtt tccagggcta tgaaacgggc gaccagtgtc gaaataacca
      901 aactcgaaaa acactgcacg tttcttgcca caaccgccag cgcagcgccg ttcataggac
      961 tttttggcac cgtatggggg attatgaatg cgttcatggg cctgagcacg acacattcat
     1021 cgagcattca agcggtggcg cccggcatcg ccgaggcttt gatcgccaca gcggtaggtt
     1081 tggctgctgc cattccggcc ctcatggcct acaactactt cgtccaacag atcaaggtgc
     1141 tggctgttga gatggacaat ttttctcatg agtttctcaa tatagctgag cgccacttct
     1201 tcaagtaata aaagaggttc agatggcaat ggatacctcc tcgtcgcgtg acagcacgac
     1261 gatttcgcag attaacgtta caccgcttgt tgacgttatg ttggtgctgc tggtcatttt
     1321 catggtgacc gcacccatta ttcaacaagg agtacaggtt aatttgccgc aagctaaagc
     1381 ggctgccatt ccgggaaccg atgacctgct cgtcgtcagc attgctaaga atggtaggat
     1441 ctatttaaat gataacgtca tgaccttgga tgaattgggc gctaagatgc gcgcgattcg
     1501 taccaatttc gccgataggc aagtttacgt cagggcggac caggacgttc gctatggtct
     1561 cgtcatgcaa accatagcgg agttaaagca agccggtatc gaacgagtgg gaatggtgac
     1621 tcgtccgacg ttagaagacg gatagcaatg gcaggtgagt ccaacagcac aagtactgcg
     1681 tcaggacatg agccgcttac agacgatcgg ctgtctaact ggttgctttt ctcgttcctg
     1741 ttacatgggg cgattctgat cgcgctgatt ttctcgccct atttcccttc caatacgcgt
     1801 cccgcgacac cggtttatac cgtcgactta gtcggaggag aaaagatcgg cggcaaccga
     1861 tccggcaccg aacttaatcc tgccgcgagc cctaaagatt ttcctaaaaa ggttagatcc
     1921 gaacccccag ccccggctcc tgaaatcaag cagtccaaac cgaaggtgca ggaaaaatcg
     1981 gagaaggctg tgaaagctga gacccccaag ccggaaaaaa gtgagaaact ggaaaagcct
     2041 gagaaagtag tcaaacccac tgagaaggcg cctgctcacg aggaccaggt agcgctaaag
     2101 aagacgccgg tgaaaaagga agaacctaaa aaggaagaac ttaaaaagcc cgaacctaga
     2161 agtgagccgg ccaaagagtc taaggagatc aaaaataccg cggcgccgga aggaggcgct
     2221 atggataaag ttcgtgaaag actcattcag tccgcgctcg atcgagtgaa aagccgtgct
     2281 gaaagcacac agaaagccgc tggcacttcc aaagcgggag aggttctcag tgtcggtcca
     2341 ggagaagggg acggcgccac cggattgggg caaggtggtc ggggcggtgg gacggcggtt
     2401 agaggtgctg aatttttcat gtaccaaggt aaaatcgtta acacgatcaa ggaaaactgg
     2461 gcatggccgg ggcagaaggg aagtttaaag gccttggtca gattcagcat caaggacaat
     2521 ggggaaatta ccgggttaaa aatagctgaa ccatctggcg acgcttcgtt cgacgagtcg
     2581 atcttgcgcg ccttgagaaa atccagtccc ctccctccgc cacctgaatc gtaccggaag
     2641 gacttcgcgc aggtagaaat gaatttccag ccgtagtttg agggatgatg tgaagcaaaa
     2701 aattacttca gcggttattt tctatctgct catggcctca gccagctatg cgcaagtgcg
     2761 tctagacgtc attggtccgg ggatgccgaa gtatccgata gccatttcgc cgctcaaaaa
     2821 tgccggcaat ggtgccgatc caggccgtat atcggaaggg gttgccgacg ctattgttta
     2881 tgatcttgat ctctccggtt ggtttcgagt tttagatcga gcaacttaca tcgaacatcc
     2941 gcagcgcagt ggtgacacgc tggaaacaat tgatttcaaa gactggtcaa cgattggcgc
     3001 tgtgggactg gtaaaaggca acttttctgt ccaaggcgat gaagttaccg tagagcttcg
     3061 gctgttcgac gttactcaag ccagacaaac gattggcaag aggtacaccg ggcgcgcacg
     3121 ggattttcgc cgcatcgcgc acaaatttgc cgacgaaatc attaaccagt tcaccggcgt
     3181 gccgggcatc tttaatacac gtatcgctta tacatccacc ggtggtggtc gtttcaagga
     3241 gattcacctt gcccatctcg acggcagcga gaagtatcag gttactaaca atcgcacgat
     3301 caatttgtcg ccgtcgtgga caccggacgg aaaatccatc ctctacacct cttacaagga
     3361 gagaaatccg agtctttatc tgttcgaact gtttagcggc aaagacttaa aattttccag
     3421 ccgaaccgga ctcaatctcg gtggcaaatg gtcgcccgat ggtcaacatg tggcggtttc
     3481 actcgagcgc ggcggaaata ccgatattta cctgttggaa aagagtggcg atattctgcg
     3541 ccggctcaca gaagatacct ctatcgatgt ttcgccctca tggtcgccag acggtcggca
     3601 actcgttttc gtgtcggacc gcagcgggtc gccgcaactc tatattatgg atgttgccag
     3661 cggcaaatcc cggcgcttga cctacggcgg gagttacaat acttcaccgg actggtcccc
     3721 ggcgggcgat cggatcgcct ttataagccg ggtcggcggt cggttttcaa tttttagcat
     3781 ccccgtcggt ggcggggaac cgacgcgact taccacgaat gcattcgata atgaggatcc
     3841 ggcctggtca cccgatgggc gcttcattgt attctcctca aaccgtgctg gccggtatca
     3901 cttatacgtg atgcaagcca cgggtgaaaa tcaacgtaga ttgacaggtt ctgggggaga
     3961 tgatacaaag ccgagctggt ctcctcggtt agaataacct cttcaatttc aaatcgattc
     4021 tgcgcaagtt ctcagaaaag gagttacgat gggcgatttt agaatgctgt gctacggtat
     4081 cgttgctgct tttattttac tgagcggagg atgcgctcca tctgctgtta agcctgacga
     4141 tggaagtgtc ggttccacag gatcatcatc tgttactcct tcgggatctg atggccagag
     4201 tgccggagat gggcgagctg gcgctacaaa ggaatccaat gttccttcta cttcgtcgag
     4261 tctagatcag ctgaaggcgg ggaaaccccc cgtcacctct gcttcaagcc cattaaaaga
     4321 tgtcctattc gaattcgaca gctacgatct caggggaaat gctcgagatg tactcaggag
     4381 taatgcagat tggcttaaga gcaatccgtc ggctcgcatt gaggtggaag ggcattgcga
     4441 tgaacgaggg acaagcgaat ataacctggc gcttggagct aaacggtccc aaaccgcgaa
     4501 agattatctc gtgagcctag gcatttctcc cgagcgcgta tcgacgatca gttatggcga
     4561 agagattccg gtgtgcacgg aacacaatga aaactgctgg cgccagaatc ggcgtgcgcg
     4621 tttcgtcgtg gtcccgagtc gaccagcatc ctaatagctg cggctagtca cgttcgcgag
     4681 gtcagacaag atgctaattc atcgaatcgc cttaatagct tttttttctg tcggcgtaac
     4741 tgcttgtgcc caaccgcagc aaattgagtt gctggagcgt gaacaacggc gaattcgcag
     4801 cgatcatgcc acaattcaca acgacgtgga gtctatgcgc tcaagcctgg ccgataccag
     4861 agcgaatatg cagcagctac aacgtgagtt tagcgcgctc aaagaaagaa tcgaagagac
     4921 acgctatcaa gtgggtcggc agattgggca atccagccga gatggcgatc agcgcgtcaa
     4981 agacttggaa gcgcgtgtcg caaaattaac cgaagcattc aaagaacagg acggccagct
     5041 aaaagcgcgc gaggaagaac taagggagct tcaaaaaaat actcggcagc agcagcctgt
     5101 ggcaggcgat acttctgcgg aaaaatcggg aatctcagct gcagaaaacg agacagtgag
     5161 aagcgactac gaagtcgcgt ggcgagcttt ggagaaaaag gattaccggc ttgcgattac
     5221 ccgcttcaag gaatttctcc aaaagcatcc gaaaagcaag ttggcgaatc aggctcaata
     5281 ctggatcggc gaaagtcact atgctttgcg ggaattcgac caagcgattc ttgagtttga
     5341 cgcggtccgc agccgatacc cgcaagcgga caaggtgccc gccgctcttc tgaaacaagg
     5401 cttcgcattc gcggaactgg gtgaaaaggt aaatgctcga ttgatcctgc aggaagtggt
     5461 cgagaagtac gcacaatcgc cggaagcggt gcaagcgaag caaaaactaa gatcactcga
     5521 gtcataattc gaccgccctg gtgatgcacc ctcttgtttt cggttgactg cgaagcaatc
     5581 gatatttacg cacctattcg gcgagaccgg aactcgagag atatggaagt tttccgcgac
     5641 atcgatgatt ttcatcgatc tgatttgaag accgtcgtca ctatggggaa ttttgacggc
     5701 atccatctcg gtcatcaggc cctcgttagc aatgccgtga cagaagccag acgatggcgc
     5761 accgaatgta tggtttttac tttcgagccg cacccattga aattgctcgc gcccgaccgt
     5821 gccccgcaga tgattgtaaa ccatcaggac aagatggaca tgctagaggc gctcggcgtc
     5881 gatgccgttg tcgtgcagac gtttgatcgc gatttcgcga atattgaagc taaagatttt
     5941 gtgcgacgct atcttgttga acatcttaat ctgtccaaga tctggttggg gagagactta
     6001 agattcggcc gtgctcgtaa gggagacgcg gaggacctga ttcgttgggg tcgtgaactt
     6061 ggttttggtg tcggtatcgt tgagcccatt ctcattcagg gaaaacggat aagcagttcg
     6121 cgaatccggc aattgatcca agaaggtcgc gtggatgagg tccggccgat gctcggccgc
     6181 taccacttta tttccggcag ggttgttagg ggtcagcgcc gtggccgaaa cattggtttc
     6241 ccaacagcta atttgtcggc cgctaccgag cttcttcctc aagatggtat ctacgcaacc
     6301 atcctgcaat ttcaagggcg gcgccggttg agcgtgagca gcgtgggtca taatcctaca
     6361 tttggggaag ggttgcatac tatagaggct tatatttttg actttgcaca ggacatctac
     6421 ggcgaaccgg taaagcttag ttttgttcag aggattaggg aagagcggaa attcgctcaa
     6481 atagaagatc ttgtgactca gatacaccag gatgtcctcg cggcacgtgc gatcttcgat
     6541 cagatgaatc tcgggtacga gaattgatgg attcatcgat tgctgttcaa cagactatgg
     6601 ttggggagaa agcatccggc atgcgtctgg acctctttct caggcagcat ctgattaatg
     6661 aagctgagcg tcttggcttg actcgctcgc ggatgcagaa gttgatcgaa gatgggacca
     6721 taaccgttaa tggggcgcca acgagatcga gtaggcgcct cagacccagc gattcggtcg
     6781 aggtacgtac aattccaaat aatatagaaa caaaattgag gccggaagat gtggtcctcg
     6841 acgttattta tgaagatcgc gactgcattg ttatcaataa ggcgcccggg atggtggtgc
     6901 atccagcttc tgggaggata agtggaactc tggttaacgc gctattgcat cattgtcctg
     6961 agctaaaaga aatcggcgcc gatggccgcc cgggaattgt tcatcgactg gacaaagata
     7021 cttctggagt aatggtcgtt gcgaaaaatc atcgatcgtt acagatcttg gcgcagcagt
     7081 tcaaagaccg gacggttcag aaggagtacc ttgccctggt ttggggtaag tttaatagtg
     7141 acagcggcat cattgatcgc ccaatcggtc gccatcgata ccagcggaag aagatgtcct
     7201 gccgtttcgc cgattcaaga aagcgcgaag ccttaaccga gtggaatgtt gaaagatgct
     7261 tggaactaag agccgattcg agaagcacca gatgggtcag cttatgtcga ctcagaccca
     7321 aaactggacg gacacaccag attcgcgtcc acctggcgga acttggtcat cccgtgatcg
     7381 gcgataaggt ttacgggcgg cttgccaagc ctgacgtgaa gaatagtacg accttggcgt
     7441 tgttgtatga ttttcctcgt caggcgctgc acgcggaaaa acttgaaatt acccatccta
     7501 ccactggtta ccgcatccag ttcaaagcgg ctctctacaa cgacatatac gaagtcataa
     7561 atgaagcaga gaaatggcgt cgtccgatcg atcggggttg acaagcaagc tggtttccaa
     7621 taggatgggt gacgttagaa ccaattcagc gtttctgaat cgacccccac aataaaaat
//
LOCUS       DASUVS010000035         4042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_9139, whole genome shotgun sequence.
ACCESSION   DASUVS010000035 DASUVS010000000
VERSION     DASUVS010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9139"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1009..1170)
                     /locus_tag="VF182_02225"
     CDS             complement(1009..1170)
                     /locus_tag="VF182_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435918.1"
                     /translation="MVTADSKCPKCGNGIMKLFPETKPLIPEPGESELRARCTACSYE
                     DTVTIRSDD"
     gene            1260..1964
                     /locus_tag="VF182_02230"
     CDS             1260..1964
                     /locus_tag="VF182_02230"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX6435919.1"
                     /translation="MTAIMCARFTASFEFREIKIRFRLQSDLPSFARRYNIAPSQEVP
                     LIVQNGGVNELKRMRWGLVRSWASDRSIANRMINVRAETISEKSSLRRLIERRRCLIP
                     ADGFYEWRREGNNKIPVWFHLKKKEPFAFAGLWDISRDVKGEMLHSFTIITTVPNTLL
                     RGIHNRMPVMFDPLQGKQWLDPRLSTRAGDIAAVLAPFPSELMESHDVSPLVNKPEFD
                     SADCIIPISDAQLRLV"
     gene            2666..2896
                     /locus_tag="VF182_02235"
     CDS             2666..2896
                     /locus_tag="VF182_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX6435920.1"
                     /translation="MRHFLATHRRVLALHGLSPEYEWVRLSGKGKVYSFVTYCRARHP
                     SHEDKVPYNVSLVDLDEGPRLSATSSRARRIK"
     gene            complement(3014..>4042)
                     /locus_tag="VF182_02240"
     CDS             complement(3014..>4042)
                     /locus_tag="VF182_02240"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6435921.1"
                     /translation="IDADAHVVESERTWDYLEGGDRRYRPVPVTVEMPLDKKRNFWII
                     GGRLIGGRDNIGKDTSQESREMADIEARLKHMDEIGTGIQVLYPTLFLRPWTDQPEIE
                     QALSRSYNRWLSDIWHKAKDRLRWAVHLPLMTMDKALDELRWASGNGACAVFMRGLET
                     RHRLHDPYFFPLYEEASRLNMPVGVHSGVGNFAVSEAFGGDPFREAKLGVIGAFHAMI
                     MRGIPERFPRLRIGAIEVSAQWIPYLVHDISLRLPKFLGKEPNPDVLRDSSQLFVACQ
                     TDDDLPYVLKYSGENSLMIGSDYGHADNASELLALNRLKANGEIDSSVIDKILYDNPA
                     RFYGLSIA"
BASE COUNT          928 a         1042 c         1083 g          989 t
ORIGIN      
        1 ttttttattg cttaactcca gatttggcga taagcgttgg gcatcccaag cacatattcg
       61 ccgagccgga atctactgac gtgctatatc aactgcggtt caggcgtgga gaaaaatgct
      121 acaaacgatt ctggtcgtcg aagatgaggc tcctaatcta cgattaatct cttatttttt
      181 agagcagcaa ggttattttg tgctccaggc gcgagatggc ttggaagcta tggagctaat
      241 gcgtcagttc agagttgata ttgtcctgtc tgatctcaga atgcccagga tggatggtct
      301 agctcttacg cgccacatcc tttcgagatt gcccagtacg cccgtgttag tgatgactgc
      361 ttacgcggga gaagatatca aatccctatc agaactccgg gtgccagtcc tgaataagcc
      421 gtttaggctg gatctgttaa actcggagat taaatcggtg cttggctccg gcaggatcac
      481 tggctgttaa gtctacagct ctcacgcgga gagcggatcc caacgtgtaa gttgtcgact
      541 cgatctccac aggtacatgc ctgcgcctaa attctgcaca ttttgcattg tgaaacttgc
      601 ctcgaagaac cggcggcgct tcatatgatg cggcatcgcc gaagcaccta acgaccacac
      661 cacgatggat tgggattttg gtactggcga tcgcacggat gatttaccga caagcgaatc
      721 cgatctgatg attccgctga gcgcgttggt tcacgatttt ggcatttttg ggcctctgga
      781 ggttcaggct gctgcggcgt aaagacgtag tcgtttaatc ggattccaag catgttgttg
      841 atgaggtgaa tagcgcgatt aacccaaaat ggacggtcct atcgggattt cggctgagat
      901 gctcgttgac attcaatact gctgcgacgg accgccctgt tgttctcgtg ttctgcagga
      961 tggtctcacc atcgctaagc acaaatctta atgtaggagt ggtcgcgtct aatcgtccga
     1021 gcggatcgtc accgtatcct cgtaactgca agcggtgcag cgggcgcgca actcgctctc
     1081 gcccggttcc ggaatcagag gctttgtctc gggaaacagt ttcatgatgc cattgccgca
     1141 ttttggacac ttactatctg ctgtaaccat ctgagttctc cttcgttgat agccgaacta
     1201 ctgtgtagag gttaaacaat ctcctgcact gatgaaaggt atcgccatgc atgctataga
     1261 tgactgccat tatgtgtgca cgctttaccg cttcattcga gtttcgtgaa atcaaaatcc
     1321 gatttaggct ccagagcgat ctgccttcct ttgcccgccg ctataatatc gcgccatcgc
     1381 aggaagtgcc cctcatagtt cagaacggcg gtgtgaacga gcttaagcgc atgcgctggg
     1441 gcctggttcg gtcatgggct tcagatcgat cgattgccaa ccgcatgatc aatgtgcgtg
     1501 ccgaaacgat tagcgaaaag tctagcttgc gccggctaat cgaacgccgg cgctgtctga
     1561 ttccggcgga tggcttttat gaatggcgcc gcgagggaaa taacaaaatt ccggtttggt
     1621 ttcacctgaa gaaaaaggag ccgttcgcgt ttgccggcct ttgggatata tcgcgcgacg
     1681 tcaaagggga gatgctgcac agttttacaa ttatcacgac cgtgccaaac acgttgttgc
     1741 gcggcattca caaccgaatg ccggtgatgt ttgatccgct tcagggaaag cagtggctgg
     1801 atccgcgtct cagtacccgc gccggggata ttgctgcggt gcttgcaccg tttccttccg
     1861 agctaatgga atctcatgat gtctcgccat tggttaataa gcctgagttc gactctgcgg
     1921 attgcatcat tccgatatcg gatgcgcagt tacggcttgt ctaagcccat ccggtagcgg
     1981 ctcgaattct tctaaggcac actaccgaat cctgtgaggc gtccgtttgt gaccgattgc
     2041 tttaccagct atggtgaacc gctgcggccg aatgatcttc gtgacaaacg atttcgacag
     2101 ccatcgagag ccgattttta gatacgcaaa ataaaagaat cacgcatgag gatggaggca
     2161 tcaatgaagc agaatgcgaa acgcaacttg caacgccgca agcggcatgc ggctcgactt
     2221 gctaagcgag agtatttcag gaaaaagaaa gaggctccgg agcgcaacgc ttaatcggcg
     2281 atcggctgcg tgagccatgc ggtgtttgtt cgagacgcag gaaatggatc acgctgtcga
     2341 gcagggctta agatttgcga cgcgcgcgct gctttaaagt ttcataatca tctttgagaa
     2401 cctcccgatc catcaatcgg taagcagccg gccgaagtaa tcacattctt tgatactttc
     2461 gatcgtcgat cagcacgcga tgttagcttt ttacgaaatg tgattgtacg caaaaccagt
     2521 ggaaaactaa ctggctagag tatgaagtca tggagtactg ggatcatgag tacgtttttc
     2581 gatccataaa tcttgactgt tctttgttga gggtacccat gcgtgaaatc agcggccggt
     2641 acgccattgc cgggcgctgc ggcgcgtgcg gcacttttta gctacccacc gcagggttct
     2701 tgccctgcac ggcctttctc cggagtacga gtgggttcgg ctcagcggca aagggaaagt
     2761 ctattcgttc gtcacctatt gccgggcccg gcacccgtcc cacgaggaca aggtgcctta
     2821 caacgtttcg ttggtggatc tcgacgaagg accgcgcttg tcagcaacgt catcgagggc
     2881 acgccgaatc aagtgacaat cggtatgccg gtggaagtgg cttacgagga ccacgaaggc
     2941 tacacattgc caaatttcgc ccgatgcgtt gagttgttgc cccggatagc tgcagcggtt
     3001 cttctactga ggctcaagca atcgacaaac catagaaccg cgctgggttg tcgtagagaa
     3061 ttttatcgat cacagaggaa tcgatttcgc cgttcgcttt aagcctattc aaagccagca
     3121 gttcactggc attgtcggca tggccgtagt cagagccgat catcagactg ttttcaccgg
     3181 aatacttcag cacgtacggc agatcatcat cggtctggca ggcaacgaac agctggctgc
     3241 tatcgcgcag cacatcggga ttgggttctt ttccgagaaa tttcggcaac cgcaggctga
     3301 tgtcatgaac caagtacgga atccattgcg cggaaacttc gatggcgccg atgcgaagac
     3361 gggggaaacg ttccgggatg ccgcgcatga tcatagcgtg aaaggcgcca atcactccga
     3421 gcttggcttc gcgaaaagga tcaccgccga aagcttcgga gaccgcgaag tttcccaccc
     3481 ctgagtgaac gccaaccggc atgttgagcc ggcttgcttc ctcgtacagc ggaaagaaat
     3541 agggatcgtg caacctatgc cttgtttcga gtccgcgcat gaaaacagcg caggcgccgt
     3601 ttccggaggc ccagcgaagc tcgtcgagcg ctttgtccat tgtcatcaac ggtagatgca
     3661 ctgcccaacg caaccgatcc ttggctttat gccagatgtc agaaagccag cgattataag
     3721 agcggctcag cgcttgctcg atctccggtt ggtccgtcca cgggcgtaaa aagagagtcg
     3781 ggtagagcac ctgaatcccc gtgccgatct cgtccatgtg ctttaggcgc gcctcgatgt
     3841 cggccatctc ccgagactct tgagacgtat ccttaccgat gttatcgcgc ccgccaatta
     3901 ggcggccgcc gataatccag aagtttctct tcttatccaa aggcatttcg acggtaacag
     3961 gcaccggccg gtagcgccgg tcgccacctt ccaaatagtc ccaagttcgt tcgctctcaa
     4021 ctacatgagc atcagcatcg at
//
LOCUS       DASUVS010000036         4571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_446270, whole genome shotgun sequence.
ACCESSION   DASUVS010000036 DASUVS010000000
VERSION     DASUVS010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446270"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1252)
                     /locus_tag="VF182_02245"
     CDS             complement(158..1252)
                     /locus_tag="VF182_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6435922.1"
                     /translation="MNANVGAIVRLSLQRIRCAAVTLAVLLLANLAPLQAEAQEKDWN
                     AVIAAAKKEGKVVVYGTSSFREILDKAKPIFEERYGIKVEALTGRAREVGERVQTEVR
                     TKRQNADLLQAGADITLPNVFRADALDRWLPPSLKVVHPDILKMIGAPQLPFTPHYVN
                     LRSILINRNRVSAKDEPKSWKDLANPKWKGAILMDDPRSAGAGHALFVGTLLHPELGE
                     DFHRKLAQNKPVFLGAGTYQQIASRVAQGEFAIGFPVDAEAVVEYAGSPVKWLPLPEG
                     ATHSIMATSLVKNAPRPNAAKLLLEFTLSEEFQSIAAAQNVTPARKGVKAAREEWSID
                     KVRLLPFAGTDSREERDKLYRLSEAIYGVR"
     gene            complement(1451..4285)
                     /locus_tag="VF182_02250"
     CDS             complement(1451..4285)
                     /locus_tag="VF182_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6435923.1"
                     /translation="MNDTRTPLHPAAANLERASGGLAHFPAVEKWDDWVEYDTAQWPR
                     KIEKHCRIIPTICFNCEAACGLVAIVEKDSHRIRRFEGNPEHPGSRGRNCAKGPATLN
                     QVTDPERIKYPLRRVGKRGEGKWERVSWDEVLDDIATRVRKALHEERRNEIMYHVGRP
                     GHELLYHQRILHSWGIDGHNSHTNVCSAGARAGYALWSGIDRPSPDHANARFMLLLSS
                     HLETGHYFNPHAQRIIEGKERGAKICVIDTRLSNTASKADYWLSSWPGSEAAILLALC
                     NVLLQEDLFDRDFVRRWINWEEYLRHEHPDKPQTFLSFTSVLKHLYADYTPEFAAKEA
                     GVDPRVLVEVAHEVARAGSAFSAHVWRNAAAGNLGGWQVARALQFLVVLVGAVGTPGG
                     TAPNAANKYIPAPPLMPPPAKVWNELLLPREYPLAFFEMSFLLPHFIKEGRGKLAMYF
                     TRVYNPVWTNPDGMSWIEMLTDENKVERHACLTPTWSETAWFADYVLPMGHGSERHDL
                     MSQETQAARWIGFRQPVLRVALEKEGKKFDLTYQAHAEAGLGEVWEEDEFWIELSWRV
                     DPDGSLGIRKYFESPYEPGKKMTLDDYYRWIFENSIPGLPAAAAEQGLTPLEYMRKFG
                     AFLIEDNVYNVHETGLKPQDMEGATVDPATSIVAKNGVALGVEVDGKAYAGFPTPSRK
                     LEFYSKTLKDWSWPEYAIPGYIKSHVHPDNIDFNAGEMLLLPTFRLPTLIHTRSGNAK
                     WLYEISHRNPIWLNSKDAEVLDVKTGDLLKINTEIGYFVDKVWVTEGIKPGVIACSHH
                     LGRWRLQEKSGGERWSTALVDLKQTEPGKWLMRHVHGVQPFTSEDPDSERIFWQDGGV
                     HQNLTFPVQPDFISGQHCWHQKVRVTKPGAGDKYGDVFVDTNKSHEVYKKWLLKTRPA
                     PGPDNLRRPYWLPRSYKPALEAYYLKKDEG"
     gene            complement(4298..>4571)
                     /locus_tag="VF182_02255"
     CDS             complement(4298..>4571)
                     /locus_tag="VF182_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435924.1"
                     /translation="LISVGMIFAELFLKHGADDAVRAGELLTKGALRKSFWVFVIGLG
                     TILPILVILLPIGSVFPNVIAAILALFGLWMYEHLWIKAGQALPLS"
BASE COUNT         1013 a         1261 c         1219 g         1078 t
ORIGIN      
        1 acatcagaaa aggcggcaag actagaaagg caaccaagat caagagaccg gtagccagta
       61 cggttctcag gcgaatcacg aagtttttca aaaagtaccg gcatcaacag aattcaaaaa
      121 actactctgt tgatcacacg gtcctgagaa cgccgattta gcggacgccg taaatggctt
      181 cggacaagcg atacaatttg tctcgttctt ctcgagagtc ggtgcccgcg aaggggagca
      241 atcggacctt gtcaattgac cattcctcgc gggccgcttt cacgcctttc ctggcgggag
      301 taacattctg cgcggcggca atgctctgaa actcttccga gagcgtgaac tccagaagca
      361 actttgcggc gtttggcctg ggagcattct ttaccaaaga agtggccata atgctgtgcg
      421 tcgctccttc aggcaggggt agccatttca ctggagaacc cgcgtactcc actacagctt
      481 cggcatccac cggaaagccg atggcaaatt cgccttgggc cacgcgcgaa gcgatctgtt
      541 gataggtgcc ggcgccgaga aacaccggct tgttttgagc gagcttgcgg tggaaatctt
      601 ccccgagttc cgggtgaagt agtgtgccga caaatagcgc gtgacctgcg ccggcggagc
      661 gcggatcgtc catcaggatt gcgcccttcc atttgggatt agcaaggtcc ttccaagact
      721 ttggctcgtc tttggcagag acacggttcc gatttatcag tatcgatctt aagttcacgt
      781 aatgcggtgt aaacggtaac tgcggcgcgc cgatcatctt caagatatcc ggatgcacga
      841 ccttaaggga cggcggcagc catcgatcca gagcatcggc acggaacaca ttgggcagtg
      901 tgatgtcagc ccccgcctga agcaagtcgg cgttctgccg tttggtccgg acttctgttt
      961 gtacccgctc tccgacttct ctggcgcggc cggtgagagc ttcgactttg attccatagc
     1021 gctcttcaaa aataggtttt gccttatcga gaatctcccg gaacgagctg gttccgtaaa
     1081 caaccacttt gccttccttt ttggctgcag cgatcacggc attccagtct ttttcctgag
     1141 cttcggcctg gagcggggct agattagcca gcagtaggac cgccagcgtt acggcggcac
     1201 aacggattcg ctgaaggctt aatctcacaa tggcacccac gttcgcattc atttgcttat
     1261 ttcctctcga cacgtttaac ggagatttag gcgcgctcac aaatatatga atagggtaca
     1321 accttcaata attacccact gaaataaatt aacccaagag cctgagatgt taagccgaac
     1381 gcgaaactgt gtcatggaca cagccggact ttgtgatctt tgcgcgcttt ggtgcgaaat
     1441 tttttgctgt tcatccttca tcctttttta aataataggc ctctaacgcc ggcttgtatg
     1501 agcggggcag ccagtaaggg cgccgcaggt tgtccggtcc cggtgccggg cgggttttca
     1561 gcagccactt tttgtagacc tcatgggatt tgttggtgtc gacgaaaaca tcgccgtact
     1621 tgtcaccggc gccaggtttc gtcacgcgca ccttttgatg ccagcagtgc tggccgctga
     1681 tgaagtccgg ctgtaccgga aaggtcagat tctgatgcac gccgccgtct tgccaaaaaa
     1741 ttctttccga atcgggatcc tcgcttgtga aaggttggac gccgtgaacg tggcgcatca
     1801 accatttgcc cggttctgtt tgcttcagat cgactaacgc ggtggaccag cgttcgccgc
     1861 cggatttttc ttgcagtcgc cagcggccga gatgatgcga gcacgcgatt acgccgggct
     1921 tgattccttc cgtcacccag actttgtcga caaagtagcc aatttcggtg ttgatcttaa
     1981 gaagatcgcc ggtcttaaca tcaagaactt ccgcatcttt ggaattgagc cagatcgggt
     2041 tgcgatggga aatctcgtac aaccatttgg cgttaccgga acgggtatgg atcagggtcg
     2101 gcaaccggaa tgtcggcaac agcaacatct caccggcgtt aaaatcgata ttgtcggggt
     2161 gaacgtggct tttgatataa cccggtattg catattccgg ccagctccaa tcttttaatg
     2221 ttttcgaata gaactccaat tttcgcgaag gcgtcgggaa accggcgtac gccttgccgt
     2281 ctacttctac gcccagagca acgccattct ttgcgacaat gctggttgcc ggatcgacgg
     2341 ttgctccttc catgtcttgc ggtttgagcc ctgtctcgtg aacattataa acattgtctt
     2401 cgatcaagaa ggcgccgaat ttacgcatgt actcgagcgg cgtcaaccct tgttctgcgg
     2461 cggccgccgg aaggccggga atgctgttct caaaaatcca ccgatagtaa tcgtccagcg
     2521 tcatcttttt acccggctcg tacggtgatt cgaaatattt tcgaataccg agagatccgt
     2581 cgggatcaac ccgccatgag agttcgatcc aaaattcgtc ttcttcccaa acctcaccga
     2641 ggccggcttc ggcgtgcgcc tggtaagtca gatcaaattt tttgccttct ttttctaaag
     2701 ccactcttaa cacaggttgg cgaaaaccaa tccagcgggc ggcctgggtt tcctgggaca
     2761 tcaaatcatg gcgttcggat ccatggccca tgggcagaac gtaatcggca aaccaggcag
     2821 tctcgctcca cgttggcgtc agacatgcat gacgttcgac tttattttca tcggtaagca
     2881 tctcgatcca actcatgccg tcgggattgg tccaaaccgg attgtagacg cgggtaaagt
     2941 acatcgccag tttgccgcga ccttctttga tgaaatgggg caggagaaaa ctcatttcga
     3001 aaaacgccag aggatactcc cgcggcaata gcagctcatt ccagactttt gccggcggcg
     3061 gcatcagcgg cggcgccggg atgtacttgt tggcggcgtt tggcgctgta ccaccgggtg
     3121 ttccgacagc cccgacgagc accaccaaaa attgtagcgc gcgcgccacc tgccaaccgc
     3181 cgaggttgcc ggcagcggca ttgcgccaca catgcgccga aaaagccgag cccgcccgag
     3241 cgacttcatg ggctacttcg acgagaactc gcggatcgac accagcttct ttagcggcaa
     3301 actcgggggt atagtctgcg tagagatgct tcaagaccga tgtaaacgac agaaacgtct
     3361 gcggcttgtc aggatgctca tggcgcaaat actcttccca attaatccat ctcctgacaa
     3421 agtcacgatc gaagagatct tcctgcagga gaacattgca cagcgcgaga agaatcgccg
     3481 cctcgctgcc cggccatgat gacagccagt aatctgcttt cgacgccgta ttggataggc
     3541 gtgtatcgat cacacagatc ttggcgccac gctccttgcc ttcgatgata cgctgggcgt
     3601 gcggattgaa ataatgtccg gtttcaagat gagagctgag caacagcata aagcgagcat
     3661 tggcgtgatc cggcgaaggc cggtcgatgc ccgaccacaa ggcatagccg gcgcgagcgc
     3721 cggctgagca gacgttggtg tggctgttat ggccgtcgat gccccacgaa tgtaagatcc
     3781 tctgatggta gagcaattcg tgtcccggtc tgccgacgtg gtacataatt tcgttgcggc
     3841 gctcttcgtg aagcgctttg cgcacgcgag tcgcaatgtc atcgagaacc tcgtcccatg
     3901 agacccgttc ccatttgcct tcgccccgtt tgccgacccg cctgagcgga tacttgatgc
     3961 gctccggatc ggtcacttgg ttaagcgttg ccgggccttt ggcgcagttg cgtcctctgc
     4021 tgcccggatg ttcggggttg ccttcgaagc gtcgaatacg atggctgtct ttctcgacga
     4081 tggcgactag accacaagcc gcctcacaat tgaaacagat ggtcggaatg atccggcaat
     4141 gtttctcaat cttgcgcggc cattgagcgg tgtcgtattc cacccaatca tcccatttct
     4201 cgacagcggg aaaatgggcc agtccgccgc ttgcgcgttc gaggttggct gcagccggat
     4261 gaagtggggt ccgagtgtca ttcatttgaa atccttctta gcttaatggt agcgcctggc
     4321 ccgccttgat ccacagatgc tcgtacatcc acaagccaaa cagcgccaaa atcgcggcga
     4381 tcacatttgg gaacaccgaa cctatgggca ataggatcac cagtatcggc aggatggtgc
     4441 cgagcccgat cacgaatacc caaaatgatt ttcgcagcgc tcctttggtc aacaattcgc
     4501 ccgctcgcac tgcatcgtcg gcgccatgct tcaaaaacag ctcggcaaaa atcatcccga
     4561 cgctgatgag c
//
LOCUS       DASUVS010000037        11064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_883585, whole genome shotgun sequence.
ACCESSION   DASUVS010000037 DASUVS010000000
VERSION     DASUVS010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11064
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883585"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1111)
                     /locus_tag="VF182_02260"
     CDS             complement(2..1111)
                     /locus_tag="VF182_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX6435925.1"
                     /translation="MSLPPKLNPPTRTLLGPGPSPVSSRVLKALALPVVGHLDPEFLK
                     IMDQNMAMLREVFQTKNRLALPMSGTGSAGMETCFVNVVEPGDSVLIGVNGVFGTRMV
                     DVAERCGAQVETVQAEWGSPLDPQQFRDALAKKKYKLTAVVHAETSTGVLQPLDEIAR
                     LVHENGSLLLVDTVTSLGGAPVRVDELGIDICYSGTQKCLGCPPGLSPVTFNERAIEV
                     IHNRKRKVQSWYLDLSMIEKYWGSERVYHHTAPISMNYALHEALRILLEEGLDASWQR
                     HRQVHETFVREMHKLELEPAVAESFRAPMINTVKIPERIDDAKVRQRLYDEFNIEIGA
                     GLGPLKGKVWRVGLMGHGSRVENVELLAKALKQLI"
     gene            complement(1132..2019)
                     /locus_tag="VF182_02265"
     CDS             complement(1132..2019)
                     /locus_tag="VF182_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX6435926.1"
                     /translation="MKICHYDNNQAGVIVEDKVYNIGDALIKAGLARNGYTMLEIIDA
                     LANNSKAMQVTREASENGSGIPVNSVKLLAPITNPGSLWAAAANYRAHQKEMIERMGS
                     ADRVTKSKDELMAEFFLKTTSSIIGPGDTVILPKISKLVDFECELCAVIGKHARKVTE
                     EQALDYIFGYTICWDISQRDPWGKGVQNTRNIRKGFDTFSALGPWIVTRDDIDEPQNL
                     TIKVLQNGKEAMTAHTSDMICGLRDHIRFLTSCLTLRPGDLITTGTPAGVSKLNDGDH
                     LHGTIEKIGSMELHVKAEE"
     gene            complement(2245..3285)
                     /locus_tag="VF182_02270"
     CDS             complement(2245..3285)
                     /locus_tag="VF182_02270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6435927.1"
                     /translation="MDRYSMKFGAHYLPTYVPDLDGSLPEYYRTMFSQMEEMDQLGYD
                     QVWMTEHHFAYYGGTVPHPPTFMAAVARTTKQIRLGVAINVLPLHNPIDIAESYAMVD
                     VISNGRLDFGVGKGSESHEYRKFGVDQQEATGRMYEGTEVILQAWSDKPVSFRGEFFK
                     YENVPVFPKPVQRPYPRFWVGCARSEDSFRWAGEKNFHLMTLPYIYREPDVLPKFVKS
                     YRDAQAQAGHDFTQTDVLGKFHIYVSGSLDQAIREAEPYLNNYFDVHAVADPDRKEQG
                     LLTQRDARTQLAEGFVLAGDPQRVVDTIRKWRDEVGLTTISGTFHFGGMPQELALKNI
                     RLFAEKVMPELK"
     gene            3869..4318
                     /locus_tag="VF182_02275"
     CDS             3869..4318
                     /locus_tag="VF182_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435928.1"
                     /translation="MILQHWKSLVASDPVDTGLAQSQFTRAPSAEEKLMLAVLENAIE
                     QFQEYALAEDPKGKALLREVEEWLWDKDSDWFFSFESICETLKFSPDYLRQGLVLWKK
                     AKIQGREITTRQQPTVRRPTKAVAAMSRLQGRYGSQCPRSDQFRLDN"
     gene            complement(4387..4692)
                     /locus_tag="VF182_02280"
     CDS             complement(4387..4692)
                     /locus_tag="VF182_02280"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEX6435929.1"
                     /translation="MIGEFGHLWERLPGILKTIVVIPAGYVLDLLDTPTSWMVRPMIA
                     VATLNLIGVRLHSPHMPVIRGQVILGSAVSLYFTLTVAAALAGYFLAIVAANIAIFF"
     gene            5253..6341
                     /locus_tag="VF182_02285"
     CDS             5253..6341
                     /locus_tag="VF182_02285"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6435930.1"
                     /translation="MPTIDSDAHVVESEHTWDFMESADQKYRPMIVRPRGENGGEYWF
                     IDGKIRGLVRIVMTAQEMAEVSSRTGRDMRTPRETREMENVQARLDHMDELGIDVQVL
                     YPTIFIEQITDKPEWEIAICKGYNRWLADIYRQGRGRLRWICVLPLLDINASLVELKF
                     CQQNGACGVFMRGIEGHRLITDPYFYPLYEEMSRLNMAVGVHVGNGNPQMLDLVAQYN
                     GGGSFWKFRIPVIGAFHSVMMSEVPTLFPQLRFHWAEAAAQWIPYVVKDLQRRWATQG
                     KELPENPLREYRQFVSCQTDDDVDYVMKYSGEDNLTIGTDYGHNDQSTEVEALRNLKT
                     AGMISATQYEKITYHNPKALFAIGENAV"
     gene            6562..7074
                     /locus_tag="VF182_02290"
     CDS             6562..7074
                     /locus_tag="VF182_02290"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6435931.1"
                     /translation="MAINSKAFERQEQEKVEYDWGTLWPKYHKEKMNWATGFRTKGDV
                     DILFLSGSTGRDPFEDGPCRTPEEERSGKGKVVGGIKEQTRATLEDIKASLEQMGATL
                     KNIVMFRYFLRRREDVFDMRDEMYKFFEEHEPDLRQHPRPATLLRDVGIDLPDMRIEI
                     EAWAVVPRKK"
     gene            7231..8034
                     /locus_tag="VF182_02295"
     CDS             7231..8034
                     /locus_tag="VF182_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6435932.1"
                     /translation="MAEIQHLAICTKNNRRLARFYQLIFGLEESWNRFQNSPYAFYMS
                     DGYLNLNCLQIRSGSSYNRIVDGQEIMPDVGINHIGFTVKSVNEIERRLASLDPPVKL
                     VASPQDGRYEEWRITDPDGIVFEIAEGGWEAGGEKRLPAIRYVGIRSEDPERLASFYQ
                     SALPIRETSRVTDRIGAAGAIFLSAGKTSLGLLKKTSASKNGIERLGFQVSSVDAIER
                     RLRNAPPYLYPGEPEVKVMAARSEDSGKSLYLKDPDGNIVELSEEGWIT"
     gene            8041..9048
                     /locus_tag="VF182_02300"
     CDS             8041..9048
                     /locus_tag="VF182_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435933.1"
                     /translation="MADPSITLACRNYDGTNAIIRGALRPQGMDLQVKEVNDVVKMFS
                     GMFRGEYDVAEMSLAEMIYYLTRDRCDFIGIPVFPSRLFRHSFMLSNRMAGICGPGDL
                     TGKKIGGLRWVQTAFIWLRGMLVDEHNLSAKDTDWYVSALHHWHDKGPEEKITPRDGS
                     VAKILTGEGSDEYETSCRALVDGKIDVLMTTENRKYDWLAADPRVRPLFPNVREAEIS
                     YYKKAGIIPIMHVLVARRSVVEGYPQLPAKLFELFCQAKKLGREWMRSVPSLNLAWKN
                     QYLDEERGLFEGRDPWAYGLKANFFALTKFLSYCDAQGITARPITPYDLFTPSTWELS
                     E"
     gene            complement(9052..10014)
                     /locus_tag="VF182_02305"
     CDS             complement(9052..10014)
                     /locus_tag="VF182_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435934.1"
                     /translation="MKLLFFVLAVSAALFNSAYTFGQALETVRIGIPSPSLSVLALRT
                     AQVKNLFREEGLEVSLIQLATNVTITALTTKEVDYATASVAAMRSAISGLPVKTVLFV
                     VRRPLHVLVARPEISSPQELRGKIVSIGAYNDLTDFVLRAILARYNVDREKDIKNLVI
                     SGSGSRLTALLTGTIDGAILPPPFNIEAEKKGFKRLASGSDVYEGGITGLSTHTDKLK
                     DNPDQIRKMIRALLKSHSFLKSNKTESIKLISDWLKMDSSVASASYDMYLNALSDDGL
                     VNDRALMFDIARAREAMKIKEEVPLSRVVDFSLVKESSGRPSNR"
     gene            10143..>11064
                     /locus_tag="VF182_02310"
     CDS             10143..>11064
                     /locus_tag="VF182_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435935.1"
                     /translation="MIAIQTHLFRIYLLAVLFSCLPLTFAAAAERVVMASLSPGLFEF
                     PIEIATMRGFFKDEGLEVVKVQMQPQVAVMALVAGDVEYSLSWGSALRAAITGVPLRV
                     VAGIAARPLHVLMARPEIQSGKDLKDKRIGVDSFAGTMEYLSRVAAKHYGLDPNRDIK
                     VLVTGPSPNRVAALKANLIDATPIDIAFAVKAEEEGLKRLINLADIVDLPISGVAVLE
                     KKLATDRAQVKKVLRAVLRGTRFMKENRQETLKMMANFLAITPAQADRAYNASIRSFT
                     DDGMISDKGLGLNLELTKERLKIKKDVPLSQ"
BASE COUNT         2679 a         2820 c         2930 g         2635 t
ORIGIN      
        1 attagattaa ctgtttcagc gccttcgcca ataactcgac attttccact cgcgagccat
       61 gccccatcaa acccacacgc cagactttgc ctttgagcgg tccaaggccc gcgccgatct
      121 cgatattgaa ttcatcgtac aaacgctgac gcactttagc atcatcgatg cgctctggaa
      181 tttttaccgt attgatcatt ggcgcccgaa acgattcggc tactgccggt tcaagttcca
      241 gcttgtgcat ttctcgaaca aacgtttcat gcacttgccg atgacgctgc caacttgcgt
      301 cgagaccttc ttcgaggaga atacgaagcg cttcatgcaa tgcataattc atcgagatgg
      361 gcgcagtatg atgatagact cgctcgctgc cccagtattt ctcaatcatc gatagatcga
      421 gataccagct ttgaacctta cgtttccgat tatgaattac ttcgatggcg cgctcgttga
      481 aagtgaccgg tgacagtccg ggcgggcaac ccaggcattt ttgtgtgccg ctgtagcaga
      541 tatcaatacc taactcatcg acgcgcactg gagcgccgcc cagagaagtt acagtatcaa
      601 ctagcagcaa gctgccgttc tcatgcacca gtcttgcgat ttcatccagc ggttgcagaa
      661 ctccggttga tgtttcggcg tgaaccaccg ccgtcagttt gtacttcttc ttcgctaatg
      721 catcgcggaa ttgttgtggg tcaaggggtg agccccactc agcttggacc gtctccactt
      781 gggcgccgca acgttcagca acatcgacca tgcgcgtgcc gaaaacgccg ttgactccga
      841 tcaaaaccga atcgcctggc tcgacgacat tgacgaagca agtttccata cccgcgctgc
      901 ccgtaccgga catgggcagc gcgaggcgat ttttggtttg aaacacctcc cggagcattg
      961 ccatgttttg atccatgatc tttaagaact ccggatcgag atgtcccacg accggtaatg
     1021 caagcgcttt caggactctc gaagaaactg gactgggacc gggtccaagc aatgtgcgag
     1081 tggggggatt aagtttcggt ggtaaagaca taattacctt gcaatctgcg cttactcctc
     1141 cgcctttaca tggagttcca ttgagccgat tttctcaatc gtgccgtgga gatggtcccc
     1201 atcgttgagc ttgctgacgc cggcaggtgt gccggtggtg atcagatcac ccgggcggag
     1261 cgtgagacag ctggtgagaa aacggatatg atcgcgcaac ccgcaaatca tatcgctggt
     1321 atgcgccgtc atcgcttctt tgccgttttg taaaactttt attgtcagat tttgcggttc
     1381 atcgatatca tcccgggtca cgatccatgg acccaaagcg ctgaatgtat cgaaaccttt
     1441 gcggatattg cgggtgttct gcacgccctt gccccaagga tcgcgctggc tgatgtccca
     1501 gcagattgta taaccaaaga tataatcgag cgcctgttct tcggtcactt tacgtgcgtg
     1561 cttaccgatg acggcgcaga gctcgcactc aaaatcaacg agcttggaaa ttttcggcaa
     1621 aattaccgtg tcaccaggac cgatgatgga cgacgtcgtc ttcagaaaaa actcggccat
     1681 cagctcgtct ttgcttttgg tgactcggtc tgcgctgccc atacgctcga tcatctcttt
     1741 ttggtgcgcc cggtaatttg ctgccgcagc ccacaatgat cctgggttgg taatgggcgc
     1801 caaaagcttc accgaattga ctggaatccc tgagccgttt tccgaagcct ctcgtgttac
     1861 ctgcatcgct ttagaattat tggccaatgc atcgatgatt tcgagcatcg tgtaaccgtt
     1921 acgagcaagt ccagccttaa tcaaggcatc gccaatattg tagactttgt cttcgacgat
     1981 aacgccggct tgattgttat cgtagtgaca gattttcatc cgatcctccg atccgcgatg
     2041 cgcggtagat ccgcttgcag cttcaacgtc tcatatctga aggtcccagc aaagtccaat
     2101 gatgggcctc accaaaggct gcaccgttaa ctgccgcagg agctcatgac agcattcaag
     2161 tcgactgctg agcggatatt gaaaaaacaa agctcagggc gggagctcga gcgttgaata
     2221 acgggcctga gacacgactg atttctactt caactcgggc atgacctttt cagcaaagag
     2281 gcggatattt ttcaaggcaa gttcttgcgg catgccgccg aaatgaaacg tgccggaaat
     2341 ggtcgtcagg ccaacttcgt cgcgccattt gcgaatcgta tccactaccc gctgcggatc
     2401 ccccgccagc acaaagcctt cggccagttg agtcctcgca tcccgctgag ttaacaggcc
     2461 ttgctccttc cgatccgggt ccgctaccgc atgaacgtca aaatagttat tgagataggg
     2521 ctctgcctcg cgaatcgctt gatccaggct gcccgacaca taaatatgaa acttccccag
     2581 tacatcggtt tgggtgaagt catgcccggc ttgcgcctgt gcatcgcgat aacttttgac
     2641 gaacttcggc aacacatcgg gctcgcgata gatataaggt agcgtcatca agtgaaagtt
     2701 tttctcaccc gcccaacgga atgaatcttc gctgcgggcg caacctaccc agaatcttgg
     2761 atagggacgc tgcaccggct ttgggaaaac cggcacattc tcgtatttaa aaaattcgcc
     2821 gcgaaaacta actggtttgt cggaccacgc ctgaagaatg acctcggttc cttcatacat
     2881 gcgtcccgtt gcttcttgtt ggtcgacgcc gaattttcga tattcatggg attcgctgcc
     2941 cttgcccacg ccaaaatcca atcgcccgtt tgagataaca tcgaccatgg cgtaggactc
     3001 ggcaatgtca atcggattat gcagcggcag gacgttaatc gccactccga ggcgaatctg
     3061 cttggttgtg cgcgcaactg ccgccatgaa cgttggagga tgaggcaccg tacctccata
     3121 atatgcaaaa tgatgttcgg tcatccaaac ttgatcgtaa cccagctgat ccatttcctc
     3181 catttggctg aacattgttc gataatattc gggaagagaa ccatcgagat cgggaacata
     3241 ggtaggtaaa taatgcgccc cgaatttcat tgagtagcga tccatgaatt acgattcctc
     3301 caagtgaaac cagcattaaa gctaaatata taggttgggt gaacttcgga gtcaacgatc
     3361 tgttgaggcc gcgttaaacc tttcttgtag gctgcggctc aggaaaagca aattcctgtg
     3421 acttctcacg ataagactga caaaaccaac ctcattcatc taacattggc agcgatgacc
     3481 tccacctgac agaccgacct ggaccataac ggcgtacctt ccttaaatct tcactctgga
     3541 agagggcgcc gtagtccctc gaaattcttc aataacttgc atcgggcata tgaggcaaat
     3601 atgccggtga cttttgtcat accgagttct cttttttcat gagtaggaga gtaagctgcg
     3661 cggcacatcg gttgcgctag ctccgaacga aggcttgatc caggaaattt cctgaccagg
     3721 ttttcactca caggcatggg caggcagagc acccgtaaca ttacatcggg cgagctctcc
     3781 agcgatcgag tctctcctgc ccgcctgaag acacattcga gacgagagca ttataaattt
     3841 gcggcactgc cgcttgagga cctagtacat gattcttcaa cactggaagt cccttgttgc
     3901 gagcgatccg gtggataccg gcctggcgca gagtcagttc accagagccc caagtgcaga
     3961 agaaaagctt atgctcgcgg tgttggaaaa tgcgattgaa cagttccagg agtatgccct
     4021 ggctgaagat cccaagggga aagcgttgct tcgtgaggtg gaggagtggt tgtgggacaa
     4081 ggacagtgac tggttcttct ccttcgaaag catctgcgaa accttgaagt tctcacctga
     4141 ttatctgcgc caagggttgg tcctttggaa gaaggctaag atccaaggtc gtgaaatcac
     4201 gacgcgtcag cagccaacgg tgcggcggcc aaccaaagct gttgctgcaa tgtcgagatt
     4261 gcagggcagg tacggttcgc aatgcccgcg ctctgaccaa ttccgcctcg ataactgagc
     4321 agatatatcc ctatatccgt taatcatcga atacctggcc ccgcatcaat gcgcccagcg
     4381 cgcctatcaa aaaaaaatcg cgatgttcgc ggcaacgatc gcgaggaagt aacccgccag
     4441 cgcagcggcg acagtaagag taaaatagag cgacactgct gaaccaagaa ttacttgacc
     4501 tcggatgacg ggcatatggg gcgagtgcag tcgtacaccg atcaaattca acgttgcaac
     4561 ggcgatcatg ggccgaacca tccacgaagt cggcgtgtca agcaggtcaa gtacatatcc
     4621 cgccggaata acgacgattg tcttaagaat gccaggaagt cgctcccaca aatggccaaa
     4681 ctctccaatc actactctta ctgctcacgt ttgttcttcc tcgaccgtaa tagaaagtca
     4741 ccgtagactt ggaagctgat taaccggtca ctgcctttag gaccgacaag gcttggagat
     4801 cttttagcag tggtcttgtc gacggctatc tctcacttca ttcgtaaaac gggctcacct
     4861 ggctggccaa atggctgcat gaattccctc cgagtaattc gccatcacct tttttaacga
     4921 gtgtgctctt aacttctttt tcgatgaata ccggaggaga acgccggagg aaatgagtcg
     4981 ctttgcatga gccgccgcag atcgcgtaac tgaaagctgg ttttccagct ctcgttaaca
     5041 gcctatcgat ggcactagag gggccgaacc tcatgtaggc gtccagttgc tgtttagctc
     5101 tgattgtttc ttgactccat gtaaatcaaa gaataagggt gcatctaagc gatgccgaac
     5161 ggcggggaag ccccgcgaga gagcaggcgt taatgtaaga ctctgccggt tcgtttgtgg
     5221 ttgattcgtt tctaaaaggt gaggtatctg tcatgccgac catcgattct gacgctcatg
     5281 tagttgagtc agagcatacc tgggacttca tggagtcggc cgaccagaaa tatcgaccga
     5341 tgatagtccg gcctcgcggt gaaaacggtg gagaatactg gtttatcgat gggaagatac
     5401 gcggcctggt gcgcatcgtg atgacggcgc aggagatggc ggaggtttcg tctcgaaccg
     5461 gccgtgatat gcggacgccg cgcgagaccc gcgagatgga gaacgttcag gcccgactcg
     5521 atcatatgga cgagctggga atcgatgttc aggtgcttta tccgacgatt tttatcgagc
     5581 aaatcaccga taagcccgaa tgggaaatcg ctatttgcaa gggctacaat cgctggttag
     5641 ccgatattta ccggcaaggt cgaggacggc tgcgctggat ctgtgtcttg cccctgctcg
     5701 atatcaacgc ttcactggtc gaacttaaat tttgccagca gaacggcgcc tgtggcgtgt
     5761 ttatgcgtgg catcgaaggc catcggctta tcactgatcc ctatttctat ccgctgtacg
     5821 aggagatgag ccgtctcaac atggcggtgg gagtgcacgt cggtaatggc aacccgcaga
     5881 tgctggattt ggtagcgcaa tataacggcg gcggcagttt ctggaaattt cgcatacccg
     5941 tcatcggcgc atttcattcg gtaatgatga gcgaggtgcc gactttgttt ccgcagctcc
     6001 gttttcattg ggccgaagcc gccgcccaat ggataccgta cgttgtcaag gatttgcagc
     6061 gccgctgggc aacgcagggg aaggaactgc cggaaaaccc gctgagggaa tatcgtcaat
     6121 tcgtatcgtg ccaaaccgat gacgatgtcg attatgtcat gaagtactca ggagaagata
     6181 atcttacgat cggcaccgac tacggccaca atgatcaatc caccgaggtc gaagctttgc
     6241 gcaatttgaa gacggccggc atgatcagcg caactcaata cgagaaaatc acctatcata
     6301 atccaaaggc gcttttcgct attggtgaga atgcagtgtg aggactcact tgcttcttga
     6361 ttcgaaaagt cgaacccgat tatgacggca cgaactgatt catattcctc ggcgacgaat
     6421 caactcgttg ctgcagctgt aaccgtaaag cgggcttagt tggatctaag atccgtaaca
     6481 actctgtcca atttgcaatt ctgccgattt ccaggattta gtagatcgat aaattatcta
     6541 ccagaaaagg ggatcttcac catggctata aactcgaagg cattcgaaag gcaagaacaa
     6601 gaaaaagtcg aatatgattg gggcacgctc tggcccaaat accataaaga gaagatgaat
     6661 tgggcgacgg ggtttaggac caaaggcgac gttgacattc ttttcctctc agggtcgacg
     6721 ggtagagatc cgttcgaaga tggcccgtgt agaacgcctg aggaggaacg atcaggcaaa
     6781 ggcaaggtgg tgggcggcat caaggaacag acccgagcga cactcgaaga catcaaggct
     6841 agtttagagc agatgggcgc tactctgaaa aatatcgtca tgttccgtta cttcctacgc
     6901 aggcgagaag acgttttcga catgcgtgat gagatgtaca agttttttga agagcatgag
     6961 cctgatctca ggcagcatcc cagaccggcg acattgctca gagacgtagg gatcgatctg
     7021 ccggatatgc ggatcgagat cgaagcctgg gctgttgtac ctagaaaaaa atagtttggc
     7081 tgtgactgca ccgaacgtgc tgtgtggata tctcgcgtat ttcaaacaat cgatgccacg
     7141 tgtgaagcgg tagcctctat tcacacgcga gagcgtgggt ttctgaactt ttgcttggtc
     7201 gtccgcagct ggtgatttgg aggagctttt atggctgaga ttcagcattt ggcgatttgt
     7261 actaagaaca atcggcgcct ggcgagattc tatcagctga tatttggact cgaagagtct
     7321 tggaatcgtt tccagaacag cccttacgct ttttacatga gcgatggata tctaaatctc
     7381 aattgcctgc aaattcgatc cggctcatcg tacaaccgca tcgttgacgg acaggagatc
     7441 atgccggatg tgggcatcaa ccacatcggc ttcaccgtca agagcgtcaa tgagattgag
     7501 cggagactgg catcgctaga ccctccggtg aaacttgtcg ccagtccgca ggatgggcgc
     7561 tatgaagagt ggcgcatcac ggatccggac gggattgttt tcgaaattgc cgagggaggc
     7621 tgggaagcag gaggcgagaa aagactccct gcgatcaggt atgttggtat tcgcagcgag
     7681 gatccggaga gacttgccag cttctatcaa tcggccctgc cgatcaggga aaccagcaga
     7741 gtgaccgacc gaatcggcgc agcaggggcc atctttctct cagcgggcaa gacctcttta
     7801 ggattactaa agaaaacgag cgccagcaaa aatggcatcg aaagactagg ttttcaggtt
     7861 agtagcgttg atgcaattga gcgaagactg cgtaacgcgc ctccgtatct ttatccaggt
     7921 gagccggagg ttaaggtcat ggcggctcgt tcagaggatt ccggaaaatc actctatcta
     7981 aaagatcccg atggcaacat cgtcgagctc tccgaggaag gctggatcac ttgagatcga
     8041 atggctgatc cttccataac cctcgcctgc cgcaactacg acggtaccaa cgccatcatc
     8101 cgtggggcgc tcagacctca aggcatggac ctgcaggtca aagaagtcaa cgacgtcgtc
     8161 aagatgtttt caggcatgtt ccggggcgag tacgacgtcg ctgagatgtc gcttgccgaa
     8221 atgatctatt atctgacccg agatcgatgt gattttatcg gcatccccgt ctttccgtcg
     8281 agactatttc ggcatagctt tatgctctcc aaccggatgg cgggcatatg cgggccggga
     8341 gatttaaccg gcaaaaagat tggcggtctt cgctgggtcc aaaccgcttt catctggctt
     8401 cgtggcatgt tggtcgacga gcataacctg tcagcaaaag acacggattg gtatgtctcg
     8461 gctcttcatc actggcatga caaaggtccc gaagaaaaga tcacgccgcg tgacggttcg
     8521 gtcgcaaaga ttctgacagg agaagggagc gacgagtacg agacgagttg tcgcgctctc
     8581 gtcgatggaa agatcgatgt gctcatgact acggaaaatc gaaagtacga ttggcttgcg
     8641 gcggatccgc gcgtgaggcc attgtttccc aacgtccgag aagcggaaat aagttactat
     8701 aagaaggccg gaatcattcc gatcatgcat gtcctggttg cgcgccgctc agtggtcgaa
     8761 gggtatccgc aactgccggc aaaactcttc gagctttttt gccaggccaa aaagctcggc
     8821 cgcgagtgga tgcggtcggt tccgagtttg aatctggcct ggaagaacca gtatctcgat
     8881 gaggagcgcg ggcttttcga ggggcgtgat ccctgggctt atggattgaa agcgaatttt
     8941 tttgcgctga ctaagttcct ttcgtactgt gacgcgcagg gaataactgc aagaccgatc
     9001 acgccatatg atcttttcac tcccagcacg tgggagctta gcgaatgaga gttagcggtt
     9061 ggaaggtcgc cccgagcttt ctttgaccag cgagaagtcg acaactctag acaacggcac
     9121 ttcctctttg atcttcatcg cttcgcgtgc ccgtgcgata tcgaacatga gtgcacggtc
     9181 attgaccagt ccgtcatcac ttaaagcgtt caagtacatg tcgtaagaag cgctggccac
     9241 agacgaatcc atcttgagcc aatcggaaat caatttgatc gattccgttt tgttgctttt
     9301 gagaaacgag tggcttttca gtaatgcgcg aatcattttt ctgatctgat cggggttgtc
     9361 cttcaattta tcggtatgcg tgctcaaacc tgtaataccg ccctcgtaaa cgtcggatcc
     9421 ggacgccaag cgtttgaatc ccttcttttc ggcctcgata ttgaaaggcg gcggcaatat
     9481 cgctccatcg atcgttccgg tgaggagcgc ggtcaatcgt gagccgcttc cggaaatgac
     9541 gagatttttg atgtcctttt ctcgatctac attatagcgc gcgagaatgg cgcgtaggac
     9601 gaagtcggtg aggtcgttat aagcgccgat gctgacgatt ttgccgcgca gttcttgggg
     9661 agagcttatt tctggtcggg ctaccaaaac atgcaacggt cggcgcacga cgaacagtac
     9721 cgttttaacc ggcaatcccg agatcgcgct gcgcatggcg gcgaccgacg ccgtggcgta
     9781 gtcgacttcc ttggtggtca gtgccgtaat cgtgacgttg gtcgccagct ggataagaga
     9841 tacctccaat ccttcttcgc gaaaaagatt tttcacttga gctgttcgaa gcgcgaggac
     9901 agagagagaa ggactgggta taccgattcg gacggtttcc agtgcctgcc cgaaggtata
     9961 agcggaattg aagagagcgg cgctgacggc gaggacaaag aaaagcagtt tcaccacagc
    10021 accgattttt atactgcgca gatgctgtct gtcaaaagga gacacggtaa aaaaactagg
    10081 atataggcaa tatgtgtcgt aggtagccgc cattattttg tactcgcggc agcgggcgcc
    10141 caatgatcgc tattcaaacc catctatttc ggatctatct cctagccgtt ctgttctcgt
    10201 gtctaccctt gacgttcgcc gcagccgctg agcgtgtggt gatggccagt ctcagtcccg
    10261 gcctatttga gtttcccatc gagatcgcca ccatgcgcgg gtttttcaaa gatgaagggc
    10321 tcgaagtcgt caaagttcaa atgcagcctc aagtcgccgt gatggcgctc gtcgccggcg
    10381 acgtcgaata ttctctttcg tggggatcgg ctcttcgcgc ggcgattacc ggagtgccgt
    10441 tgcgagtggt cgccgggatt gccgctcgac cgcttcatgt cctgatggcg cggccggaaa
    10501 tccaatccgg caaagatctc aaggacaaaa ggatcggcgt cgactcattt gccggtacga
    10561 tggaatacct gtcccgcgtg gcggccaagc actatgggct tgatcccaat cgcgacatca
    10621 aggtgctggt taccggcccg agtcccaatc gcgtcgctgc tctaaaagca aatctcatcg
    10681 acgctacacc gattgacatc gcatttgccg ttaaggcaga ggaagaagga ttgaagcgcc
    10741 tgatcaactt agccgatatc gtcgatctgc cgatctccgg cgtcgccgta ctggaaaaaa
    10801 aattggccac ggatcgagct caggtcaaaa aggtgctgcg agccgtgctt cgaggcacgc
    10861 ggttcatgaa agagaatcgt caggaaacac tcaagatgat ggccaacttt ctcgccatca
    10921 cgcccgcgca agcggaccga gcctataacg cctcgattcg atcttttaca gacgacggca
    10981 tgatctccga taaagggttg ggtcttaatc tggagttaac caaggaacgg ctcaagataa
    11041 aaaaagatgt tcctctcagt caga
//
LOCUS       DASUVS010000038        14771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_447666, whole genome shotgun sequence.
ACCESSION   DASUVS010000038 DASUVS010000000
VERSION     DASUVS010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14771
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447666"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            435..1013
                     /locus_tag="VF182_02315"
     CDS             435..1013
                     /locus_tag="VF182_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX6435936.1"
                     /translation="MTQNLDGLKVAILVAEGFEQVELTEPKKALESAGAQTRIVSPVK
                     DKVQGWNHFDKADRFTVDMPLSEADARDFDALLLPGGVANPDQLRIIPEAIKFVREFF
                     SANKPVAAICHGPWPLIETGIVRGRTMTSWPSLKTDLINAGAKWVDQEVVTDDGLVTS
                     RRPDDIPAFNRKMIEAFAEGKRKHEQSAARAS"
     gene            1560..2432
                     /locus_tag="VF182_02320"
     CDS             1560..2432
                     /locus_tag="VF182_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX6435937.1"
                     /translation="MEYFEPKSVDEALSLLGKHGAEAKVIAGGTDVMVDIKYKEEPGC
                     LINIKKIPGLTGIRENGGSLRIGPLATIREIETSAIVREKLPVLWEASHQFASLQVRN
                     TATIGGNICRASPSGETLAPLLVLEATADCAFSDGQRNQPFMTFFQGPGKSVLGGKGL
                     LTEIDVPYPAVGSKGAYLKHAVRGAMDIAMVGVAVLITPDAAKSNIQDVRIGLGAVAP
                     TPVRAPKTEALLRGKPLTAALLKEAGALAATEASPIDDQRSSAENRRWIVEALTRRGL
                     EQTWKAATGKEIAS"
     gene            2429..2905
                     /locus_tag="VF182_02325"
     CDS             2429..2905
                     /locus_tag="VF182_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6435938.1"
                     /translation="MSVTIATKINGQSVTASADASTSLLEFLRDTLEMKGSKLCCNTG
                     ECGACTVIYNGKPVNSCVTMAADAIGAEIVTIEGLADGDKLHPVQQAFIDTGAVQCGY
                     CTPGYIMSVKALLDRTTKPTPADIEEAVSGNICRCTGYNKIVDAIHLAADRIARRA"
     gene            2909..5197
                     /locus_tag="VF182_02330"
     CDS             2909..5197
                     /locus_tag="VF182_02330"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6435939.1"
                     /translation="MAKAEFKVVGTSPARMGGIERVVGKGIYGIDLMLKDQLHGAILR
                     SQYAHAKIVSIDTSEAKKIPGVHAIITAADAPNVRYGRSYMDRHILARDKVRFMGDPV
                     AAVAAESPAIAKRALKRIKVVYEPLPVALDPEEATKPEAPSLHEDMPLPKTIPADAGL
                     KNICGHAVVHVGDAEKAMAEADIVVDEVYETKMVHPQYLEPRIAAAQMEPNGRLTVWA
                     NAQAPFPVRQEVAKMLGLSLNNVRVISAGLGGGFGGKGSGITSGAAVEPICGLLAIKA
                     KRPVMIVLDKAEETISTTIRSGARMYIKTGVKKDGTIVARTGKVFYDAGGYSGFGVQA
                     GGRCTNMLGGWYLMPNCHIDGYSVYTNKQVCGPVRGPGGPQAAFAVESHMDSIAAKLG
                     MDPIEFRLKNVPKAGDKIVGVPKLRDVSLGETIRIAKEKIGWGKVKLEKNQGIGLATG
                     SWIESAGPGGGAIVKVNEDGSVTVHIGKIDMGTIPGFGIPMIVAEELGIPVSDVTVVN
                     VDTDASPWDAGTVGSRGILVSGTASRLAAIDARNQIFKMAARQLEASPDDLEIVDKQI
                     RVKGTPAKAVALATVATNAHNVIGEVIGRGYCDNLELMAEEKAHGSSQPFTAHACIVE
                     VSPDTGNVKILKYVAVHDIGQPIHQAAVEGQIEGAAAMSIGQALCEQVVFDKNGRTLN
                     PSFVDYLMPTINMMPRIETTLVSGYPGAGPYGAKGAGEIACVPPMACIANAIFNAIGV
                     RIYKLPLSPENVLRGLKAAGKA"
     gene            complement(5506..5970)
                     /locus_tag="VF182_02335"
     CDS             complement(5506..5970)
                     /locus_tag="VF182_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014652742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6435940.1"
                     /translation="MEIERKLAELGIEVPAPPLPAAHYIPYVQVGNLLFVGGNTGRLN
                     DQPHLYAGKVGDRVTLEQAYQMARHCALNHLAIIKSAVGDLDRVVRFVKLLGFVNVAP
                     GFTKMPQVVNGASDLFIEIWGERGQHARAAVGVASLANEAPVETEVIVQIRD"
     gene            complement(6387..6935)
                     /locus_tag="VF182_02340"
     CDS             complement(6387..6935)
                     /locus_tag="VF182_02340"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX6435941.1"
                     /translation="MSVIRSKIILGSVVLACLTVFGCRNAEEIATRDELPDINKQLRA
                     YVTIASTQVRTGPGPQFRATGEVPPRAKVHVVGRDGDWVLIVSKKGNIPGYIEMASVK
                     PGEGEESASGEPTVGGKYEILANTRLHSGPGLHYPVVAEVSKGTIINVVEEDKGWIKV
                     ESKRGKQPGYVEANLAKPAPDQ"
     gene            complement(7026..7445)
                     /locus_tag="VF182_02345"
     CDS             complement(7026..7445)
                     /locus_tag="VF182_02345"
                     /inference="COORDINATES: protein motif:HMM:NF020489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonyl-tRNA synthetase editing
                     domain-containing protein"
                     /protein_id="HEX6435942.1"
                     /translation="MRLLCFQAKRFRWKTHSKTLPEIPDLDVEREIADGVVIFVQAEA
                     TDTSEQHRDSIRRKGVKHIKWLANKRELKNVVLHSFTHLGGDTAEPRFAEALIQSIAD
                     RLNQSGYHVQITPFGYFCEWDLSVYGESLAKVWKEIH"
     gene            complement(7458..8027)
                     /locus_tag="VF182_02350"
     CDS             complement(7458..8027)
                     /locus_tag="VF182_02350"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX6435943.1"
                     /translation="MILLIDNYDSFTYNLYHYLGELGADVKVYLNNKITLDQIAELKP
                     EKIVISPGPCTPKEAGISCAVIRRFGAQIPTLGVCLGHQAIGAAFGGEIIRAPSIMHG
                     KLSEVAHDSQTIFRSIKNPFAAMRYHSLVINRQSLPEDLTVSAQTPDGVIMAVRHKKM
                     PVEGVQFHPESILAEEGKKLLQNFLEYYR"
     gene            complement(8024..9511)
                     /gene="trpE"
                     /locus_tag="VF182_02355"
     CDS             complement(8024..9511)
                     /gene="trpE"
                     /locus_tag="VF182_02355"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX6435944.1"
                     /translation="MIHPTFEEFCRLAEQGNLIPVYQELLMDLETPLSFFKRLERDRY
                     AFLLESVEGSERWARYSFLGSRPRLVFKSRGKQVEIIEDGKKRKLEAEMPLESLKTLV
                     GNYRPVTVPGVPPFFGGALGYVAYDAVEQFHGIANNKNHPFDAPEIFFLFVQTLIAFD
                     NLKHTIKIIDNVHLETGTNLETAYAEATANIRKIISSLQKKPRSIEPRELSDGKAQKQ
                     FRSNLDPDGFQKAVIRAKEYIQAGDIIQTVLCQRLETETVADAFEIYRALRFINPSPY
                     MFYLELEDLRIIGSSPETMVRLSGDTIELRPIAGTRPRGATPEEEQALEADLLGDPKE
                     RAEHIMLVDLGRNDVGRIAEIGTVEVNELMGIERYSHVIHIVSNVRGKLAADKSPFDL
                     FVSAFPAGTVSGAPKIRAMQIISELEPEKRGLYAGAIGYFGYNGNLDTCIVIRTIVMK
                     GSKIFINAGAGIVADSDPDMEYQETLNKARAMFRAVELAEQWSKA"
     gene            9894..10673
                     /locus_tag="VF182_02360"
     CDS             9894..10673
                     /locus_tag="VF182_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX6435945.1"
                     /translation="MVRREKSNYVIQSVSHALDVLEQFNGNVDEIGVTELSKRLKLHK
                     NNVFRLLATLEARGYIEQNKISENYRLGLKCLQLGQTFIHQMGLLLQARATLEDLAKS
                     AKESAFVAIRKGAGIVPLDFVEPARPVRVVSFLGSVLPPHCTAVGKVYLAFESDGGVS
                     LGDLDPLKRYNDKTITDTRGLAQQVKETGEIGYAIDDGEFINEISSIAVPVRDYTRSL
                     VGALAIVGPSDRLNLEKLRNDIAPLILGAGRDLSRRLGYAG"
     gene            10903..12897
                     /locus_tag="VF182_02365"
     CDS             10903..12897
                     /locus_tag="VF182_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX6435946.1"
                     /translation="MLTALEKIFFLLFLAASLYYGGRRVYDVYRSIARGKPDSRFDQL
                     PRRFIDALLNVVLQRSVFRTRPVVSFFHALVFYGFVFYFLVNLVDVLEGYLTLHGRGG
                     LWNLFNLVADVLTTAVLIGILGLMIRRFLVRPEDFDFPGNVPVREGVRQGIRRDSTIV
                     SVFILFHVGSRLMYKASQLAVEGTDPWQPVSTTFAALFSGTAPSSLEVLQHFFWWGAL
                     GSILVFIPYFPRTKHIHLLMAPINLAFKKKDPGVLQPMDFENEEVFGASKLEEFAWPR
                     LMDAYSCIMCNRCQDVCPATSTGKALSPAAILINERFELNDLFPKFAAGAESPRPLLE
                     FALNEEAAWACTTCNACIEVCPVGNEQMLHIIDVRRERVLSAAEFPNGLKNTFNQMET
                     AGNPWGIAAAERLVWAKDLPFPVPTIDQNANPDVLYWVGCAVSFDPRAQKIARNMAEI
                     LHSAGLNWAVLGKTEKCTGDTARRTGNEYLFAQMADENVAALNAVKAKVIVTTCPHCF
                     HTIGKEYPQFGGNYVVKHHTEFIDELIREGKLKVESNSAASVTYHDPCYLGRHNGIFD
                     QPRSLIEMTGANLIEPDRTRNNSFCCGAGGGQFWKEEEKGKERVSTNRFRELKQTGAQ
                     TVATGCPFCLRMLTEETAKEDGKGPEVFDIAELVTKNIRK"
     gene            12902..13366
                     /locus_tag="VF182_02370"
     CDS             12902..13366
                     /locus_tag="VF182_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX6435947.1"
                     /translation="MSWKSRARALALTSLFTTFSQACQAQPEVTIESAKGAVGFQVEI
                     AATPEKRERGLMYRRELPPDHGMLFIFPAEKVNSFWMKNTPIPLDMIFISRERKIVGI
                     VHETVPFSLDGRSVSAPSQYVLEINGGLSRRYGFKAGDSVRFDDVRPESVEE"
     gene            complement(13401..14468)
                     /locus_tag="VF182_02375"
     CDS             complement(13401..14468)
                     /locus_tag="VF182_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435948.1"
                     /translation="MAQKKIVHVVGTGTIGEPLIGILCTFKEQFGVDEVTFHKRTPLL
                     TDRSKVMVLCNRGAQLCVDKERRQKFIEMGMTPTYEAEEAIERASVIIDCTPVGNDNK
                     EKIYNKYANNGRGFIAQGSEYGFGKMYARGINDKALIKGEDQFVQVVSCNTHNLAVLI
                     DTIALGPEKEDNLEESRFVCMRRANDISQEGDFIPAPEAGKHDDTEFGTHQGRDAHYL
                     FKTLGMDLNIYSSALKLNTQYMHTIHFDLKLRRGIDKDQLMRRLQSNGRVAMTNKKSS
                     ASVFSFGRDHGLFGRILNQTVIVTQSLAMRGDREIIGTCFTPQDGNSLFSSLAATMWF
                     LYPDSYEDKLSPVREYFFNEI"
BASE COUNT         3342 a         3900 c         4161 g         3368 t
ORIGIN      
        1 ggcgacatgt cagatcaaca gtttgccgat gatgccgaat ccgtccggag ggatctgaac
       61 aaactgaaga gcttaattga gaaataagct tcaacgaacg cgaggatgcc gtcgctggga
      121 gctgaaattc gaatcggaga agcgggggaa aacactcgcg agcagcatat gcgggtgatc
      181 tgagagcatc gcctggccaa atcggggagc aaaggtgaga gctacaaggc gtttcgcctc
      241 agagatgatc ggtacttcac atctcctgta tcgcggctgg gatggtacgt gtgtttggtt
      301 agcgatttag ttacatagct cccatcgatg cctgaaacgt tgaggtattg cgaggcacgc
      361 tgcttgcaaa aacaaaaact tgagtcaggt tctacgatca gtcaggcagc tatttacgtt
      421 gaggaggatt gcccatgaca caaaatctgg acggactaaa ggttgccatt cttgttgccg
      481 aaggattcga gcaagtcgaa ctcaccgagc ccaagaaggc gcttgaaagc gccggcgcgc
      541 agacacggat tgtttcccca gtcaaagata aggttcaggg gtggaaccat tttgacaagg
      601 ctgaccggtt tacagtggat atgccgctca gcgaggcgga tgcgcgcgat tttgatgcgc
      661 tgctcttgcc cggtggtgtt gccaatcccg atcagttaag gatcataccg gaagccatca
      721 aatttgttcg agagtttttt tccgccaata aacctgttgc cgcgatctgt catggtccat
      781 ggccgctgat cgagacgggc attgtgcgag gccggacgat gacgtcttgg ccatcgctga
      841 aaaccgactt gatcaatgcc ggcgccaaat gggtcgatca ggaggtggtc acggacgacg
      901 gactggtcac gagccgtagg cccgatgata tcccggcatt caatcgaaag atgatcgaag
      961 cgttcgctga aggcaagcgc aagcatgagc aaagcgccgc gagagcgtca tagcgccaaa
     1021 taactacctg catcgtccaa tctagagggt tgtgagagtt ttgaggttgt ccgaagttca
     1081 atggattcat ggccatgacg ttgttgcaga ggcttcggtc tgaagacttc gttcccaggg
     1141 ttcgcggagt aaaataaacg atgctactct aattcttcac ccggtcatct ctgataccta
     1201 cattgaccgc atgcaataac tctgggcgca gccctgggga ataggcagct caaggaaata
     1261 gccgtttgtg gcgcagcagc catttacatc cgtttgaagc ccatgtggca agaggggaag
     1321 aagaagcggt gctgttgcca ggcggtgagt gaccgaactc ggcactcact tctccgggcc
     1381 gacttttgta gctgtttgcg ttcgcattcc tgatcggttt tcttaccttg tcccttgaca
     1441 gttgcaccgc tgggctaata tgaaactgac agatcgcccg agtgacgagc tgatctgttg
     1501 agtgctgtag agattgaaag ctgactatat cacctcaagt aaattatccg ggggaactga
     1561 tggaatattt tgaaccgaag tcggtcgacg aggctttatc tttactcggt aaacacggcg
     1621 ccgaagctaa ggtgattgcc ggcggcaccg acgtaatggt ggacataaaa tacaaggaag
     1681 agccgggctg cctcatcaac attaaaaaga tcccgggact caccggtata cgcgaaaatg
     1741 gtggcagctt gcgcatcggc ccattggcga cgatccgcga gatcgagacc agcgcgatcg
     1801 tgcgggaaaa actgccggtc ctctgggaag cgtcgcacca gttcgcgtcc ttacaggtgc
     1861 gcaacaccgc caccatcggc ggcaacatct gccgcgcctc gccttcgggc gaaaccttgg
     1921 cgcctttact ggtgctcgaa gcaacggccg actgcgcttt ttcggacggg caaagaaacc
     1981 aaccgttcat gacatttttt caaggaccgg gaaagtctgt tcttggcggg aaaggtctgc
     2041 tgactgaaat cgacgtgccc tatcccgccg tcggcagcaa aggcgcgtat ctgaagcatg
     2101 cggtgcgcgg cgcgatggat attgcgatgg tcggcgtggc ggtattgatc acgccggacg
     2161 ccgccaagag caatatacaa gatgtccgca tcggcttggg tgcggtggcg cccacccccg
     2221 tgcgagcgcc taagaccgaa gccttgctgc gcggcaagcc attgaccgcc gcgctgctaa
     2281 aggaagccgg cgcgttggcc gcgaccgaag cgagtccgat cgacgatcag cgcagtagtg
     2341 ccgagaaccg gcgttggatt gtcgaagcgc tgacgcgccg cggcttggaa caaacctgga
     2401 aagctgccac cggcaaggag atcgcatcat gagcgttact atagctacca aaatcaatgg
     2461 acaatcggtg actgccagcg ccgacgcatc gacttcgctc ttagagtttc tgcgcgacac
     2521 tttggaaatg aaaggcagca agctctgctg caacaccggc gagtgcggcg cctgtacggt
     2581 gatttacaac ggcaagcccg tcaactcgtg cgtgaccatg gccgccgatg ctatcggcgc
     2641 cgagatcgtt accatcgagg gattggcgga cggcgataaa ttgcatcctg tgcaacaggc
     2701 gttcatcgac accggcgcgg tgcagtgcgg ctactgcacc cctggctaca tcatgtcggt
     2761 gaaggcgctg ctcgaccgca ccaccaagcc gacgccggcg gacattgaag aggcggtctc
     2821 cggaaatatt tgccgttgta ccggctacaa caagatcgtc gacgcgattc atctcgccgc
     2881 cgatcgcatc gcgaggaggg cgtaagccat ggcaaaagct gaattcaaag tcgtcggcac
     2941 aagtccagcc agaatgggcg gcatcgagcg cgttgtcggt aagggcatct atggcatcga
     3001 cttgatgctc aaggatcaat tgcacggcgc gatcctgcgc agccaatatg cgcatgcgaa
     3061 aattgtcagc attgacacca gtgaagcgaa gaagatcccc ggcgttcatg ccatcatcac
     3121 cgcggcggat gcgccaaacg tgcgttacgg ccgcagctac atggatcgtc acattctcgc
     3181 gcgcgacaaa gtgcgcttta tgggcgaccc ggtagctgcg gtggccgccg aatcgccggc
     3241 aattgccaaa cgggcgctca agagaatcaa agtcgtctac gagccgttgc cggtggcgct
     3301 cgatcccgaa gaggcgacca aacccgaggc gccgagcttg cacgaagaca tgccgctgcc
     3361 gaaaaccatc cccgctgacg ccgggcttaa aaatatttgc ggccacgctg tggttcatgt
     3421 tggcgatgcc gaaaaggcaa tggccgaagc ggatattgtc gtcgatgaag tttatgaaac
     3481 caagatggtc catccgcagt atctggaacc gcgcatcgcc gcggcgcaga tggaaccgaa
     3541 tggccgcctc accgtctggg ccaacgccca ggcgccattc ccggtacgcc aggaggttgc
     3601 gaagatgctg ggactgtcgc tcaacaatgt gcgcgtgatc tccgccggct taggcggcgg
     3661 cttcggcggc aagggcagcg ggatcacttc gggcgcagcc gtcgagccga tctgtggcct
     3721 gttagcgatt aaagctaaac ggccggtgat gatcgtcctc gataaagccg aagaaaccat
     3781 ttcgacgacg atacgttccg gggcgcgcat gtacatcaag accggcgtca aaaaagacgg
     3841 gactattgtc gcccgcacgg gcaaagtatt ttacgacgcg gggggttact ccggtttcgg
     3901 cgtgcaggcc ggcggccgct gtaccaacat gctcggcggc tggtacttga tgcccaattg
     3961 ccatatcgac ggctacagcg tttataccaa caagcaggtc tgcggccccg tacgcggccc
     4021 tggtggtccg caagcggcgt ttgcagtcga gtcgcacatg gactccatcg ccgccaagct
     4081 gggtatggac ccgattgaat ttcgtctcaa gaacgttcct aaagccgggg acaagatcgt
     4141 cggcgtgccc aagctgcgcg acgtttcgct gggtgaaacc atccgcatcg ccaaggagaa
     4201 gatcggctgg ggcaaagtaa agctggaaaa gaatcaaggc atcgggctgg ctaccgggtc
     4261 atggatcgag agcgccggcc ccggcggtgg cgccatcgtg aaagtgaacg aagacggctc
     4321 ggtcacagtc catatcggca agatcgatat gggcacgatt cccggcttcg gtattcccat
     4381 gatcgtcgcc gaagaactcg gtattccggt aagcgacgtc accgtagtca atgtcgacac
     4441 cgacgcgagc ccgtgggacg ccggcaccgt cggcagccgc ggaattttgg tttccggcac
     4501 cgcatcacgg ctcgctgcga tcgacgcgcg caatcagatc ttcaaaatgg ccgcccgcca
     4561 gctggaagcg agcccggacg atctggaaat cgtcgacaag cagatccggg tgaaaggcac
     4621 gcccgccaaa gcggtagcgc tggcgaccgt tgcgaccaat gcgcataacg tcatcggcga
     4681 agtgatcgga cgcggctact gcgataatct cgaattgatg gccgaagaaa aagcgcacgg
     4741 gagttcgcag ccgtttaccg cgcatgcgtg catcgtcgaa gtcagtccgg acaccggcaa
     4801 tgtgaagatt ctgaaatacg tcgccgtcca cgacatcggc caaccgatcc atcaagccgc
     4861 cgtcgaaggt cagatcgaag gcgctgccgc aatgagcatc ggccaggcgc tctgtgaaca
     4921 agtcgtcttc gacaaaaatg gaagaacctt gaatccgtca ttcgtggatt acttgatgcc
     4981 gacgatcaac atgatgccgc gcatcgaaac aactctcgta tccggctatc cgggggcggg
     5041 tccctacggc gctaaaggcg ccggcgaaat tgcctgtgtt ccgccgatgg cgtgcatcgc
     5101 caacgcgatc ttcaacgcta ttggcgtgcg catttacaag ttgccgctga gccccgagaa
     5161 tgttttgcgg ggattgaaag ccgcgggtaa ggcgtagctt gcggcaacga tgtgaccgga
     5221 tcctcgccac agttcacatg agcccatcta atcgagcgat tcgtaggatt agtttgggaa
     5281 taaacgcgcc ggcgtccgta gggtagcggt tgcgcaggca atcgaggact gaacgaaagc
     5341 ttagactgca gattctggtg atttgcgcgg ggtgctgatc cacctgggtt tcagtctact
     5401 gagactccgc tggttgctcg atcaacgtgg tctgctaccg gtgccaatga tcatacactg
     5461 acctgcagct gactccatgg catgtgcaga ttagaacggc aatgcctaat ctctgatctg
     5521 cacgatcacc tcggtttcca ccggagcttc attagcgaga gaggcaactc cgactgcggc
     5581 gcgggcgtgc tgccctcgtt cgccccagat ttcgataaac agatcggacg cgccattcac
     5641 gacttgcggc attttggtaa aacctggcgc aacgtttaca aagccgagca atttgacaaa
     5701 gcgcaccacc cgatcgagat cgcccaccgc cgacttgatg attgccagat ggttgagggc
     5761 gcaatggcgc gccatctgat aggcctgttc cagggttacg cgatcgccga ctttgccggc
     5821 atataggtgc ggttggtcat tgagcctgcc ggtattaccg ccgacgaaca agaggttacc
     5881 gacctgaacg tagggaatgt aatgagccgc gggcagcggt ggtgccggta cttcgatgcc
     5941 gagttcggct aacttcctct caatctccat aaaccatctc cttccgcgca gatatgcgtg
     6001 cgattctatg cgagaaaccg gcgcaagaac aagtgtcaac aggcgggaga gccgagcccg
     6061 aggccttgaa tgaatgtctt attggtttcg aactggccag gtggcgcgca actgcgcgct
     6121 gggcgccatt ggccgccctc gcgtcgtgac ggctagccgc aattacgagt cagcacgata
     6181 ccgtcaatga aggagcgagc tgacgagctg gttcgattcg catttgcagc accataccag
     6241 agaggcgatg ttgcgagcga cggcggcgtc gttgtcaaag acaggcaggc gcttcgattg
     6301 cgatcagcga cgcgagcaac ctccggatcc ttacctaggt ttgtgtttac gggtcgcgca
     6361 gaaaagaagg tcggcctcct tcggatttat tgatcgggcg ccggtttggc taaattcgct
     6421 tcgacgtagc caggctgctt gcctcgtttt gactctacct ttatccagcc tttgtcttct
     6481 tcaacgacgt tgataatcgt cccctttgac acctccgcaa ccaccgggta gtgaagtccg
     6541 ggtccgctat gaagtcgggt atttgcgagg atttcatact ttccaccaac cgtcggttcg
     6601 ccgcttgcgc tctcttcacc ttcacccggt ttgacagagg ccatttcgat gtaacctggg
     6661 atgttgcctt tcttggacac gatcagcacc cagtctccat ctctgccgac cacgtgaacc
     6721 ttggctcgcg gcggcacttc gccagtggct ctgaactgcg gcccggggcc ggtgcggact
     6781 tgcgtgcttg caatggtcac ataagcgcgt agctgcttat tgatatccgg aagctcgtcc
     6841 cgagtcgcga tctcctcggc gtttctgcag ccgaaaacag ttaaacaggc tagtacgacc
     6901 gaccctaaaa taatcttact tcttatcacg ctcatcgtgc tcctccgatt ttttagtgac
     6961 cgattatagc acgtttcgaa caaagcggcc gaccgcctgg cgggagaaga gtcacccagc
     7021 gatggctaat gtatttcttt ccaaactttg gcgaggcttt caccataaac cgataaatcc
     7081 cactcgcaga aataaccgaa tggggtgatt tgaacgtgat aaccgctctg gttgagccgg
     7141 tccgcgatgc tttgaataag cgcctccgca aagcgcggtt ctgcagtgtc gccgccgagg
     7201 tgagtaaatg aatggagaac aacgtttttc agttcccgtt tattcgccag ccatttgatg
     7261 tgcttgaccc ctttgcgtcg gatcgagtcg cgatgttgct ctgaagtatc ggtagcttcg
     7321 gcttggacga aaataactac gccatcggca atttctcgct cgacgtcgag atccgggatt
     7381 tcgggcaggg ttttagaatg agttttccaa cgaaaacgtt tggcttgaaa acacagaagt
     7441 ctcatgacaa tcgaggatta tcgatagtac tctaaaaaat tttgcaggag ttttttgcct
     7501 tcctctgcaa gtatcgattc gggatgaaac tggacgcctt cgaccggcat ttttttatgc
     7561 cgcaccgcca tgatgacgcc atccggagtt tgcgcgctga cggttagatc ttccggcagg
     7621 ctttgtcgat tgattaccag cgagtgataa cgcattgcgg caaacggatt tttgatgctg
     7681 cggaaaatgg tttgcgaatc atgagctact tccgagagct tgccatgcat gatcgagggt
     7741 gctcggataa tctcgccgcc aaacgccgct ccgatggcct ggtggcccag gcagacgccc
     7801 agcgtcggta tttgggcgcc gaaacgccgg attacggcac aggaaatacc tgcttcttta
     7861 ggcgtgcagg gtccgggaga gatgacaatc ttttccggct ttagttcggc tatctggtcc
     7921 aaagtgattt tgttgttcaa ataaaccttc acatcggccc cgagctctcc caggtaatgg
     7981 taaaggttgt aagtaaatga atcgtaattg tcgatgagca ggatcatgcc ttgctccact
     8041 gttcggcgag ctccacggcg cgaaacatcg cgcgcgcctt gttgagcgtc tcctgatatt
     8101 ccatgtcggg gtcggagtcg gcgacaatcc cggctccggc gttgatgaat attttgctgc
     8161 ctttcatgac aatggtccga atgacgatgc aggtatccag attcccgtta tagccaaagt
     8221 atccgatggc gcctgcatag aggccgcgtt tctccggctc cagctcgctg ataatttgca
     8281 tggcgcgaat tttcggcgct ccggaaaccg ttccggcagg aaacgccgag acaaacaggt
     8341 caaacgggct cttatcagca gcgagcttgc cgcgtacgtt cgaaacgatg tgaatgacat
     8401 gagagtagcg ttcaataccc ataagctcgt tgacttcaac agtgccgatc tcggcgatgc
     8461 gcccgacatc gtttcgtcct agatcgacca acatgatgtg ctcagcccgc tcttttgggt
     8521 cgccaagcag gtcggcttca agcgcctgtt cttcttccgg tgtggcgccg cgcgggcggg
     8581 ttccggcaat gggccggagc tcgatggtat cgccgctcag gcgcaccatt gtttcaggcg
     8641 acgagccgat gatccgtaaa tcctccagct cgagatagaa catgtagggc gaaggattga
     8701 taaagcgcag agcccgatag atttcgaagg catcggcaac cgtttcggtt tcgagcctct
     8761 gacacaggac tgtttgaatg atatcgcctg cctggatgta ttccttggcc ctgataaccg
     8821 ctttctgaaa accgtccggg tcgagattgg agcgaaactg cttctgcgct ttgccgtcgc
     8881 tcagctcacg gggctcgatg ctgcgaggtt ttttttgcag cgacgagata attttgcgga
     8941 tattggcagt cgcttccgcg taagcggttt cgaggttggt tccggtttct aaatggacgt
     9001 tgtcgatgat cttgatggta tgtttcaggt tatcgaaggc gatcagagtt tggacgaaca
     9061 gaaagaagat ctccggcgcg tcgaacggat ggtttttgtt gttggcgatg ccgtgaaatt
     9121 gctcgaccgc atcgtaggca acatagccca gggcgccgcc gaagaacggg gggacacccg
     9181 gcaccgtaac aggtcgataa ttccccacaa gcgttttcag cgactcgaga ggcatttcgg
     9241 cttcaagctt tcttttttta ccatcttcga tgatttcgac ttgtttgcct cgacttttaa
     9301 aaacgagccg aggccggctg cccaaaaaag agtaacgcgc ccaacgttcg ctgccttcaa
     9361 cgctttcgag cagaaaagcg tagcgatccc gttcgagccg cttgaagaac gagagcggcg
     9421 tttccaaatc catcagcaat tcctgataaa ccggaatcag gttgccctgc tccgccaacc
     9481 ggcaaaattc ttcaaatgtc ggatgaatca taggtctcac aaacaaaaag ggccataagc
     9541 taccgcctac ggccctcctc aatattttat ttactgacgg aaattagcca caggccaatc
     9601 ggagcgactg ctgcctgggc caccagcttt tcaccaacac aacaaattcc gacggctcag
     9661 tcatggcgac gagtgtgccc tattgtctta tatcggtcaa gacgagccga taggaggtcc
     9721 gagtcgtctg cattcagccg gcagcagctc ctagcgagcg ctaaagaatt cgcagatgcg
     9781 acttgctttc gttgacaagg tccggctggc gatataggtt aaaacgcttt tttggaacgg
     9841 cccgtagagt ctattcgggc agcacccatg gacacaatga ggcaggagga gacatggttc
     9901 gtcgggagaa atctaattac gtgattcaat ccgtatcgca cgcccttgat gttctcgagc
     9961 agttcaacgg aaacgtcgat gagatcggcg ttaccgagtt gagcaaacga ttgaagcttc
    10021 ataagaacaa cgtttttcgt ttgctggcca cgctcgaggc gcgcggttac atcgaacaga
    10081 acaagatctc ggaaaattat cgcctggggc tcaagtgtct gcagctcggg caaacgttca
    10141 ttcatcagat gggtttgttg ttgcaggccc gcgcaactct tgaagacctg gcgaaatcag
    10201 ctaaagagag cgccttcgtg gcgatcagaa aaggcgccgg catcgtccct ctggactttg
    10261 tcgaaccagc gcggccggtg cgagttgttt cgtttttggg ctcggtcctg ccgccgcatt
    10321 gcactgcggt cggtaaggtc tatctcgctt ttgagtcgga tggaggcgta tcgcttggcg
    10381 acctcgatcc tctgaaacgc tacaacgaca agacgattac cgataccaga ggattggccc
    10441 agcaggtcaa agaaaccggc gagatcggtt atgccatcga tgatggcgag tttatcaacg
    10501 agatcagttc gattgccgtc ccggtgagag attacaccag aagcttggtc ggcgctttgg
    10561 ctattgtcgg accgagtgat cgattaaacc tggagaagct ccgaaacgac atcgcgccat
    10621 taattctcgg tgccggccgc gatctttcaa gacggttggg gtacgccgga taatgcgcta
    10681 accggatgat gcgctaatgg gattggctgc tccgcgactc ttcagaaact ccgctcgttg
    10741 attcataaat agtgcttcct gtagagtcag tcttatttgc tcgactgagt ttgcggagtg
    10801 ggtcggcgca cttgccggtc gttgaccagc ggcgcctgct caggtcgtct gcttcattca
    10861 attcgagaaa gcaaacggag accggaggtt gctaaagcac taatgcttac ggcgctcgaa
    10921 aaaatatttt tccttctatt cttggccgca tcgctctact acggcgggcg ccgagtctat
    10981 gacgtttatc gaagcatcgc tcgaggcaag cccgatagcc ggttcgatca attacccagg
    11041 cggttcattg acgctctgtt gaatgtcgtt cttcagcgct cggttttcag gacaaggccg
    11101 gtggtttctt tcttccacgc gctggtgttc tacggcttcg ttttctattt tctggtcaat
    11161 ttagtcgacg tcctcgaggg ttatctaact ttgcacggcc gcggcggttt gtggaacttg
    11221 tttaatctag tcgccgatgt tctaaccacc gctgtcctga tcggcattct cggtttgatg
    11281 atccggcgct ttctggtccg ccctgaagat ttcgattttc ctggcaacgt gccggtgcgg
    11341 gagggagttc gccaaggaat ccgccgcgat tcgacgattg tttcggtttt catccttttt
    11401 catgtgggca gccgtttaat gtacaaagcc tcccagcttg ccgtcgaagg caccgatccg
    11461 tggcaaccgg tgagcacaac atttgcggct ctattttccg ggacagctcc cagctcgctt
    11521 gaggtgctcc agcatttctt ttggtggggc gcattggggt cgatcctggt ttttattccg
    11581 tactttccgc gcaccaaaca cattcatcta ttgatggcgc cgatcaattt agcattcaag
    11641 aaaaaggatc ccggcgtatt gcagcccatg gattttgaga acgaggaagt attcggcgcg
    11701 tccaaactgg aggaattcgc ctggccgcgc ttgatggatg cgtacagctg catcatgtgt
    11761 aaccgctgtc aggatgtttg tccggcgacc agcaccggca aagcgcttag cccggcagcg
    11821 atattaatca acgaaagatt cgagctgaac gatcttttcc cgaaattcgc tgccggggca
    11881 gaaagcccgc gaccgttgct cgagttcgcc ctcaacgaag aggcggcttg ggcttgcacc
    11941 acgtgcaacg cttgtatcga ggtctgtccc gtcggcaatg aacagatgct gcacattatc
    12001 gacgtccggc gtgaacgcgt gttgagcgcc gctgaattcc cgaatggcct taaaaacacg
    12061 ttcaatcaaa tggagacggc agggaaccca tgggggattg cagcggcgga gcgtctggtt
    12121 tgggcgaagg acttgccgtt cccggttccg acaattgatc agaatgccaa tccggacgtg
    12181 ctgtattggg ttggctgcgc ggtctcgttc gacccgcgcg cacaaaaaat cgctcgcaac
    12241 atggcggaga ttctccattc cgccggcttg aactgggccg tccttgggaa gaccgaaaag
    12301 tgcaccggcg acaccgcgcg acggaccggc aacgaatatc tgtttgccca aatggccgac
    12361 gagaacgtgg ccgcgctcaa tgcagttaaa gccaaggtta tcgtcacgac ctgtccccac
    12421 tgctttcata caattggtaa agaatacccg cagttcggtg gtaactacgt agtaaaacat
    12481 cataccgagt tcatcgatga gttgatccgc gagggcaagc tcaaggtgga gtcgaactcg
    12541 gctgcctcgg taacctatca cgacccttgc tatctgggcc ggcacaatgg aattttcgat
    12601 cagcctcgtt cgttgatcga aatgacaggg gccaatctca tcgagcctga taggactcgc
    12661 aacaacagct tttgttgcgg cgcaggtggt ggacagtttt ggaaagagga agagaaaggc
    12721 aaggagaggg tgagcaccaa tcgctttcgt gagctcaagc aaaccggcgc gcaaactgtg
    12781 gcgaccggat gtccattttg tctgcgaatg cttaccgaag agacagcgaa agaagacggc
    12841 aaggggccgg aggtcttcga tattgcagaa ctggttacaa aaaatatccg aaaatagacc
    12901 cgtgtcctgg aaaagtcgcg ctagagcttt ggctttgacc tcgctattta caacattttc
    12961 acaagcttgt caggcgcaac ctgaagttac tattgaaagc gctaaaggag ctgtcggttt
    13021 tcaagttgag atcgccgcga ctccggagaa gcgcgagcgt ggtctgatgt atcggcgcga
    13081 gctgccgccg gaccacggca tgttgtttat ctttccagcc gagaaagtaa attcgttctg
    13141 gatgaagaac acgccgatcc cgttggatat gattttcatt agccgcgaaa gaaaaatcgt
    13201 cggtatcgtc catgagacgg tgccattttc tttggatggc cgttcggtat cggctccgag
    13261 tcagtacgtg cttgaaatca acggcggact ttcacgccgg tacggcttta aagccgggga
    13321 ttccgtcagg tttgatgatg tgaggccgga gagcgtggag gaatagaatg gcgccgagct
    13381 gcgcggttct ctgccacggg ttaaatttcg ttgaagaaat attcccgaac cgggctcagc
    13441 ttgtcctcat aagagtccgg atacaaaaac cacatggttg ccgccaagct actgaatagc
    13501 gaattaccgt cctgcggcgt aaagcaagtg ccgatgattt cgcggtctcc tctcatcgcc
    13561 aagctctgcg tgacaattac cgtttggttc aaaatacggc cgaacagccc atgatcccgg
    13621 ccgaacgaga ataccgaagc ggacgatttc ttgttggtca ttgcgaccct accgttggac
    13681 tgcagtcgcc gcatcaactg atctttgtcg atgccgcggc gtaatttgag gtcgaaatga
    13741 atcgtgtgca tatactgggt gtttaacttg agagccgagg aatagatatt cagatccatg
    13801 ccgagagttt tgaatagata atgcgcatct ctgccctggt gagtcccgaa ctcggtatca
    13861 tcgtgtttgc ctgcttccgg cgccgggata aaatcacctt cctgactgat gtcattagcg
    13921 cggcgcatgc agacaaagcg gctctcttct agattatctt ccttttccgg tcctaacgcg
    13981 atggtgtcga tcagcacggc caagttgtgg gtattacagc ttacaacctg gacaaactgg
    14041 tcctctcctt tgatcaaagc tttgtcattg atgccacgcg cgtacatctt gccgaagcca
    14101 tactcactcc cttgagcgat aaaaccgcgc ccgttgttag cgtacttatt ataaattttc
    14161 tctttattgt cgttgccaac cggcgtgcag tcgataataa cgctggcgcg ctcaatggct
    14221 tcctcagctt cataggtcgg cgtcataccc atttcgatga acttttgtcg ccgctcttta
    14281 tcgacgcaaa gctgcgcgcc tcgattgcat aggaccatga ccttcgaacg gtcggtgaga
    14341 agcggtgtcc gcttgtgaaa cgtcacttca tctacaccga actgctcctt gaaggtgcag
    14401 agaattccga tcaagggttc gccgatagtt ccggtgccga ccacatgaac gatttttttc
    14461 tgtgccattt gtcccgccta agttttgatg gagcggattc taacccatac gggtggaacg
    14521 atcaaccgag gagtttgagc cgtttttcga cggagcaggt cagttgaacg ctagacaaat
    14581 gtctttaaag caaaggtttg aggtggtaca aattgtttag ggagattgat atggcaaatg
    14641 agatagttcc tgcgaaatct tttttcggac agaactttgg cattgattcc aaagatctag
    14701 acaaggttct ggcgactgcg ctggcaaaga aagccgacta tgcagacctc tactttgaat
    14761 atcgcaagaa c
//
LOCUS       DASUVS010000039         8701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_10927, whole genome shotgun sequence.
ACCESSION   DASUVS010000039 DASUVS010000000
VERSION     DASUVS010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8701
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10927"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="VF182_02380"
     CDS             complement(<1..1431)
                     /locus_tag="VF182_02380"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6435949.1"
                     /translation="MSKNLSSTSFLSLLFCFVLAPAVWAQTRPIAIVGGTLIDGTGRA
                     TVEDAVVVFQDGRIQDVGKRGEVAVPAKAQTIDAKGKTILPGLIDGHCHYWEWIGELH
                     LAYGVTTCPDINNSPTEWIIAQKEGIQKGKIRGPRLWISGHALDGPRPQGMPEQRWQR
                     GSIIVRTEDEARKAVQEHVNKGMDGFKFLERIAPNVAKAAVEEARRSGKPVIGHSVNM
                     FDAIDAGYTSIEHSWAVMFTTIRDEKKRFDVDMARMTGKVGTVEAHTNMEPDQFDAII
                     KAMIEKDVHWSPAWGTQFRALSPRAAEMKRRELAVLKNPGLSYLPAYNAESIENYFAN
                     FEKMAPEKRDQLNAAYKMVQDFARRYVAAGGKIHSGSDPGSYLPAYGVHAELELMIDA
                     GFSPLVAIQSASLNVAQAWGKDKDYGSVEKGKVADLVVVRGNVSNDISATQNVETVFM
                     DGKSIDTSFHPNYRNPIPRPLSDRFVT"
     gene            2020..2442
                     /locus_tag="VF182_02385"
     CDS             2020..2442
                     /locus_tag="VF182_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6435950.1"
                     /translation="MADESKPEVVAVRSGVGIDSRQKIPYFVGVSEATTGARGLSLQY
                     VIIPPAGAAVPHIHQGYETAIYVLSGRVETTYGPGLRKSIVSGPGEFLYIAEGVPHQA
                     RNLSDTESAVGIVARNDANEQERVMPYDPSTDQTQPAS"
     gene            2841..3854
                     /locus_tag="VF182_02390"
     CDS             2841..3854
                     /locus_tag="VF182_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435951.1"
                     /translation="MRVFFALVLPILLVVPTLSSTTLALERHIHARAGGSSAQQLPLI
                     VAKDLGLFEKYGLDVDLLEIRGGSLLMQALIGQSVNSADVGAQAPIRAILSGANVVIT
                     GGLLNKTLYKFVTRKEIRKPSDLRGKKIGIVNFGGANEFNILMALKAWGMPPDSVKLL
                     PSGGSLARLAAMEVEGGLDGTIIPYGEAAMAAKKGFNILADLSELMEESPEKVFIADR
                     SFIAKKRDNAKRFFQGVSESTYILRSQPELKGKIVPIIGKWLRIPAKLAEEAYDIHHK
                     VFAFPPRVGRQGLQDVLEIIQREARRAKDDLKLSRFVDESTLDELEKEGFFKMLTEKS
                     SSK"
     gene            3969..4844
                     /locus_tag="VF182_02395"
     CDS             3969..4844
                     /locus_tag="VF182_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6435952.1"
                     /translation="MTSRIKHIGIMSQMPNRLFKFYECLFDFRTDVRTPAEERERAKQ
                     IGYKILNSGRVAGAFARTVIASDGNIGVAFNQRRPGYPGGLDHFGMTVDDIGEVLEQI
                     KEKYPSVGCVKRPPGRPFVDYSSHDPEGNIFDIAEPGKKQMVGVFSEAEAKRPRHIKH
                     FTIRAMNPEALAGFYINVFGFKEEEKALEDPNFYLTDGTVTMVLAPYKISDYLGTEHK
                     RPGFDHIGFEVEDLQGFKDDVELLAKHDPEFLAPKPANVESEYQVIMDLLRSCRYGKH
                     QMCDPEGNLVDIAVV"
     gene            5463..5660
                     /locus_tag="VF182_02400"
     CDS             5463..5660
                     /locus_tag="VF182_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435953.1"
                     /translation="MLSKAGYINNLSLCDEYRTVKDAVVEVGTWEGGMIAGIAALLGN
                     GRALYLFDSFHCLPAATEHDA"
     gene            complement(6098..8191)
                     /locus_tag="VF182_02405"
     CDS             complement(6098..8191)
                     /locus_tag="VF182_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX6435954.1"
                     /translation="MSDPTYRIPTTGLYLETDGTLVAKPKSGAPVVDATEAVAAKLTA
                     AYVGKGLQLMSGSVEAGDGVGEALKELGDAAEGIVLVGDVIELGLKIAELMGAFPHKK
                     DPVIAKLERIDAHLNSIEDLVLASWADSRRQQLAILRAHSAASLQIAMDYAEQNRPQS
                     AVWASKIAQADYNSLFAVKVFTESGVEGGFWLRPFSLKANGLSQVGMTGTPYYNQSWA
                     HFHPDRQQILTDPGKPVWDYRFTLPATVYAMVSRLAVLKAVAPRSLQTGESGCREMRG
                     YVRFLRAVVQRIQNGMWATQELPTDQYSRFLFGWNGWSPVAAAQIDSGYGFARAVFAY
                     EWSTLYPPDPAMWPAGLSEPFEQGRTPDEVRALANENINRTGSHWWSLVWRDIGMLAM
                     CRIISDLDRACTPRVFWRLIGDAQKRLIMASRDVTPARVASGLAHLTSAGDPADDSAR
                     TFRLYESLRSGNPAVRQALIRATNELLSLTPPEEPPSRPVPAGWWRGEDDARDAVGGN
                     DGRVVGSVRFVAGRDGHAFAFSEGGYVEIRNARVLEPDTITVTAWVRRDAPPSEPAYL
                     LSKGANACIAASYALYTGQTSGLLFYVSDGRVINYSADAGPGIWDNSWHFVAGSYDGE
                     FVRLFVDGREVGTPTPAVVEEIGYRLPDGDSFYLGAYRGTCESRFDEGRLDEVRLFDR
                     ALTAAEIVALYTVES"
BASE COUNT         1959 a         2448 c         2369 g         1925 t
ORIGIN      
        1 cgtcacgaat cggtctgaca acggccgcgg aatcgggttg cgatagttcg ggtgaaacga
       61 cgtatcgatg gattttccat ccatgaacac ggtttcgaca ttctgagtgg cggaaatatc
      121 gttcgagaca ttgcctcgga cgaccacaag atcggcgact ttgccttttt ccacgctgcc
      181 gtaatctttg tccttgcccc aagcctgagc gacattcaag ctggcggatt gaatcgccac
      241 cagcggactg aagcctgcgt cgatcatgag ctcgagttcc gcatgcacac cgtaagccgg
      301 taagtaactt ccaggatcgg agcccgagtg aatctttccg ccagcggcca catagcggcg
      361 cgcaaaatcc tgcaccatct tgtatgcggc gttcagttga tcgcgctttt ccggcgccat
      421 cttttcaaaa ttggcaaagt aattttctat cgattcggcg ttgtacgccg gaagataact
      481 cagtcccggg ttcttgagga ctgcgagctc gcgccttttc atctccgcgg ctctgggcga
      541 aagcgcgcga aactgcgtgc cccaagccgg actccaatgg acatcttttt cgatcatggc
      601 cttgatgatt gcatcgaatt gatcgggctc catattggta tgggcctcca ccgttccgac
      661 cttgccggtc atacgcgcca tatcgacatc aaaacgcttc ttttcatcgc gaatggttgt
      721 gaacatcacc gcccacgagt gctcgatcga cgtgtacccg gcgtcgatgg catcaaacat
      781 gttcaccgaa tgaccgatca ccggcttgcc tgaccggcgc gcttcttcga cagcggcttt
      841 ggcgacattg ggtgcaatcc gctcaagaaa cttaaagccg tccatgcctt tgttgacatg
      901 ttcctggacc gctttgcgcg cctcgtcttc agtgcggacg ataatgctgc cgcgctgcca
      961 gcgttgctcc ggcatgccct gcggccgcgg tccatcgagc gcatggccgg agatccacag
     1021 acgcggcccg cgaatctttc ccttctgaat gccttccttc tgcgcgatga tccattctgt
     1081 cgggctgttg ttgatgtcgg gacaagtggt cacgccataa gcgagatgga gctcgccgat
     1141 ccattcccaa taatggcagt gcccatcgat cagtcccggc aagatggtct tgcccttggc
     1201 atcgatggtc tgagccttgg ccggaaccgc gacttctcct ctcttgccaa cgtcttgaat
     1261 gcggccatct tgaaagacaa ccactgcgtc ttcgacggtg gcgcggcccg tgccatcgat
     1321 caaggtgccg ccgacaatcg cgatcggtcg agtttgcgcc caaactgccg gcgccaggac
     1381 gaagcagaag agcaggctta gaaaactcgt tgaggataaa ttttttgaca taccgcactc
     1441 ctattcaggt agcgtttgaa ttatcgagaa cacatacgct ttggggcata atccctactc
     1501 aacaagctaa tcctcttggt tccccgcgca gatcgatttc tggttcagat cacggctaag
     1561 caggctaata gcacaggcaa agcgacacgg acaaattttt attttctaag aagcccggcc
     1621 taatatggct ctgtttcgat tgccggctgc tcgcgacgat gaagacttca cgataagcaa
     1681 aaaggcgcct cgatgctcca tagtcgaacg atgagagaag aagctccggt gccaaacttc
     1741 tgttctgaat atcatcgttt cgctttcctt ctcaatgcct gcgtgtacgc acctcgctca
     1801 gcaaatcgac ataattacta tgagcacgaa ggcacgcaca gcagcgtctc tccatccgtt
     1861 caagcttgat ttgcccctcg tttcgcgaac tcctatccaa cgcggccacc aagcagtttt
     1921 cgggtatctt tttcgccaac gacttcacgc ggggaagcgc ttgcgctcgt agccgcgtgg
     1981 cgtaggataa cttcagctta ttattcgcag gagttaaaaa tggcagacga atctaaaccg
     2041 gaagttgtcg ctgtccgtag cggggtcggc atcgactcgc gccagaagat tccttatttc
     2101 gtgggcgttt cggaagcgac caccggcgcg cgcggactct ctctgcagta cgtgatcatt
     2161 ccacccgcgg gcgctgcagt gccgcatatt caccaaggct acgagacagc catatacgtc
     2221 ttgtcaggac gggtggagac aacctatggg ccaggattgc gcaaaagcat tgttagtggg
     2281 ccgggtgaat ttttgtacat cgccgagggc gttccacatc aagcgcgtaa cttgagcgat
     2341 acggagtctg cggttggcat tgtcgccaga aacgacgcca acgaacaaga gcgggtcatg
     2401 ccatacgatc ccagcacgga tcaaacacaa cccgcgtcct gagtcttaga tgatagtgtt
     2461 aatgtctgtg ttggtaccgg tcgctaaaag ataccagaat gaccaaggaa ggtggcggtc
     2521 ggccgcggcg ggatttcctt atagattgct ttcggcttaa ccgccaactc gagtgatctt
     2581 ctaagccgat ttgtattgct atgatttcta cgtgggcgcc tgagagaagc ttcatgaggg
     2641 gaaaataaat ttctgcccaa ttgtcgacat aacatatagt acttcacttc aaactcttcc
     2701 aatacgcggt aattcgctgt cttcgtcttg aacggttgga acctttgaat cgccggaaca
     2761 tctggaacga acggcttttt agccgttact gataggcggg aactgttgac aaccggttca
     2821 ccggcaaatt atatcgcaat atgcgagtct tcttcgcttt ggtcttaccc attctgctgg
     2881 ttgttccaac cctttcatca actacgctcg cgttagagcg ccatattcat gcccgcgccg
     2941 gaggttcgag cgcccagcaa cttccgttga tcgtcgccaa agatcttggg ctatttgaga
     3001 agtacggctt ggacgtcgat ctactcgaga tccgcggcgg ttcacttttg atgcaggcgc
     3061 taatcggcca gagcgtaaat tccgccgacg tcggcgctca ggcgccgatt cgcgccattc
     3121 tgtccggcgc gaacgtcgtg atcaccggcg gacttctcaa caaaacgctc tacaagttcg
     3181 tgacccgcaa agagatccgc aagccatcgg atctccgcgg caaaaaaatc ggcatcgtca
     3241 atttcggcgg agccaatgaa tttaacattc tgatggcgct caaggcatgg ggcatgccgc
     3301 cggattccgt gaagctgcta ccctcgggtg gcagcctggc tagactcgca gccatggagg
     3361 tcgagggagg actcgatggg acgatcatcc cctacggcga agcggctatg gcagctaaaa
     3421 aaggcttcaa catcttggca gacttaagcg agctcatgga agaatcgccc gagaaggtat
     3481 tcatcgccga ccgctccttc atcgccaaga aacgcgacaa tgccaaacgg tttttccagg
     3541 gggtgagcga atcgacgtac atattgagaa gccagcccga gctaaaagga aaaattgttc
     3601 cgatcatcgg aaagtggctg cgaattccgg caaagctcgc tgaggaagca tacgatatac
     3661 atcacaaagt cttcgctttt cctccgaggg tcgggcgcca gggcctgcaa gacgttttgg
     3721 agatcattca acgcgaagcc aggcgcgcta aagatgattt gaaattgagt cgctttgtcg
     3781 acgagagcac actggacgag ctggagaaag aaggtttttt caagatgctc acggaaaaat
     3841 cctcgagcaa ataaggcggt ccgactgcaa cacacttcac caaaacaaaa ccatcatgat
     3901 gcttgtgatt aggcgaaata gatggaatca atacactggg gtgatcaacg aagaaagaga
     3961 ggtttgacgt gacatcgaga atcaaacata tcggaatcat gagtcagatg ccgaatcggc
     4021 tgttcaaatt ttacgaatgt ctttttgatt ttagaacaga cgtgcgcacc cccgccgaag
     4081 agcgtgagcg cgccaaacag attggctaca aaatattgaa ctccggccgg gtggccggcg
     4141 cattcgctcg gacagtcatc gccagcgacg gcaacatcgg cgtcgcgttc aatcagcgac
     4201 gtcccggtta tcctggcggg ctcgatcatt tcggcatgac cgtggatgac atcggcgaag
     4261 tactcgagca gatcaaggaa aaatatccgt cagtcggttg tgtcaaacgt cctccgggcc
     4321 gtccctttgt cgattacagc agtcatgatc cggaaggaaa tattttcgat atcgccgagc
     4381 cagggaaaaa gcagatggtc ggcgtattca gcgaggcgga agccaaaaga cctcgtcaca
     4441 tcaagcattt cacgatccgc gccatgaacc ccgaagcgtt ggcgggattt tacataaacg
     4501 tctttggctt caaagaagaa gagaaggcgc tggaggatcc gaatttttat ctcaccgacg
     4561 gcacagtgac gatggtgctt gcgccttaca aaatctccga ttacctcggc acggaacaca
     4621 agcgaccggg attcgatcac atcggttttg aagtcgaaga tttgcaggga ttcaaagatg
     4681 acgtcgagct actggcgaaa cacgaccctg aatttctcgc gccgaagcca gccaacgtgg
     4741 aatccgagta ccaggtcatc atggatctgc ttcgtagctg tcgctatgga aagcaccaaa
     4801 tgtgtgaccc ggaaggaaac ctggtggata tcgccgtcgt ctaaatctcg gaatttatcg
     4861 cgcaattcgg attagctcat cggtttttat tagttcctgt tattgatttt ctatccttgc
     4921 gccgtgtggc cgagtcaaag cttccggccg ctcagagagc ttcaatatca taatgctcga
     4981 catagccgac gaagcttgaa cgtttgaccg agctagcgcg cgacaatgcg acgaactaac
     5041 caaagaaggc gtggcctgta taaaaccacg aacatctcga tctcaacgtc agaaaagtaa
     5101 tttaaaaagt gatagtgcgc tgcatcgagc tgtattgcca acctgctgcc ttgagcggtg
     5161 ttgctgtacc tgctgcatga tatttgctcg catcattttc agatagatct gggcgatccc
     5221 tggcagggtt gatttacaaa accacgctac agatgcgctc tcaattcatt cggaagtgtt
     5281 attattatgt tgactcgctc actcgataat gtccgtggca cgctggctgc cagctcgcgc
     5341 tggagtgcat ttaaaaagcg ccacccggcc atgttcaatg ggctggcgta cggataccga
     5401 gcttaccgtt cttacaagct gcttcattac ggcgtattca tccgcgtttc aaggcctgca
     5461 gtatgctgtc taaagcgggt tacattaata acctgtcgct ctgcgatgag taccgcacgg
     5521 tcaaggacgc ggtggttgaa gtgggtacat gggaaggcgg catgatcgcc gggattgccg
     5581 cgcttcttgg caacggccgt gccttatact tgtttgacag ctttcattgc ttgccggcgg
     5641 cgaccgaaca tgatgcgtag ccttccggat gctcggcggt ctcctggcaa tcggacctaa
     5701 aaaaacccga gtttgccgat atcgccgaag agaagagcct caaggtggat gtacgatatg
     5761 cgcaagaggc catgaaatta agcggcgttt ctcaaggcgg attttctatc gaagcataag
     5821 ctcgcagacg cgattcacca gaaccgtaac ggctatccgt ccattctcaa acattaggag
     5881 ctgcgacata cttagatcac tggcgccatc ccgtagaacg ttccggcttg acggttgatc
     5941 tcggatcatt ggttccgctt ggcgggctac tcgctggacc taagaattcc gctgacagtt
     6001 ggatcggcgc caccgcagtc tggtgacaag cggtcttgat cataaaaacc aagaatttgc
     6061 gaattccggg agattgccca ggaagccccc ggccatgcta gctttccact gtatagagcg
     6121 ccacaatctc cgcggcagtc agcgctcggt cgaacagacg cacctcatcg agccttccct
     6181 cgtcgaaccg cgactcgcac gtgccgcgat aagcaccgag ataaaatgag tctccgtccg
     6241 gtagccgata tccgatctct tcaaccacgg caggcgtcgg ggtgcccacc tcccggccgt
     6301 cgacaaacaa cctcacgaac tcgccatcat acgatcccgc gacgaaatgc cacgaattgt
     6361 cccagattcc cggcccggcg tccgccgaat agttaatgac ccggccgtcg gagacataaa
     6421 acaagaggcc gctcgtctgg ccggtgtaga gcgcgtagga ggccgcgatg caagcgttgg
     6481 cgcccttgga gagtaggtag gcgggttctg acggcggcgc gtcgcggcga acccaggcag
     6541 tcaccgtaat cgtgtccggc tcgagaaccc gagcgttgcg tatttccacg tagccgccct
     6601 cggagaacgc gaatgcgtgg ccgtcgcgcc cggcaacgaa gcgcacgctg ccaacgaccc
     6661 ggccgtcgtt tccgccgacc gcatccctcg catcgtcttc gccgcgccac caccccgcgg
     6721 gcacgggtcg ggacggcggc tcttcggggg gagtcagcga cagaagctca ttggtcgccc
     6781 gaatgagcgc ctgccgaact gcgggatttc ccgaccgcag cgattcatag aggcggaacg
     6841 tgcgcgccga atcgtcagcc gggtcgcccg cagatgtcag gtgcgccagc ccgctcgcca
     6901 ccctcgcagg cgtcacgtct ctggatgcca tgatcagtct tttctgcgca tcgccgatca
     6961 agcgccagaa cacgcgcggc gtgcaggccc ggtccagatc cgagatgatg cggcacatgg
     7021 cgagcatgcc gatgtcgcgc cacaccagag accaccaatg cgatcccgtc ctgttgatgt
     7081 tttcgttggc gagagcgcgc acttcatccg gcgtgcgccc ctgctcgaac ggttcggaaa
     7141 gacctgccgg ccacattgcc ggatcggggg gatataacgt cgaccactcg taggcgaaca
     7201 ccgcccgcgc gaaaccgtag ccgctatcga tctgcgcggc tgccaccggc gaccacccgt
     7261 tccagccgaa cagaaaccgg ctgtattgat cggtgggaag ttcctgggta gcccacatgc
     7321 cgttttgaat gcgttggacg acggcgcgga gaaagcgcac atagccgcgc atctcgcggc
     7381 atcccgactc ccccgtctgc aacgagcgcg gcgcgaccgc cttcagcacg gcgagccggc
     7441 tgaccatcgc gtacacggtc gccggcagtg taaaacggta atcccacacc ggctttccgg
     7501 gatcagtgag gatctgctgg cggtccgggt ggaaatgcgc ccagctctgg ttgtaataag
     7561 gcgtgccggt cattccgacc tggctcagcc cgttggcctt caggctgaac ggacgtagcc
     7621 agaacccgcc ctcgacgccg ctttcggtaa agaccttaac ggcgaaaagc gaattgtaat
     7681 ccgcctgggc gatcttgctc gcccacaccg cgctctgcgg gcggttctgc tccgcgtaat
     7741 ccatcgcgat ctgcaaggat gctgcggaat gcgcccgcag gatcgcaagc tgctggcgcc
     7801 gcgagtcggc ccaggacgcg aggaccagat cctcgatgct gtttagatgg gcatcgatgc
     7861 gctcgagctt ggcaatgacc gggtccttct tatgcgggaa agctcccatc aactcggcga
     7921 tcttgaggcc gagctcgatg acgtcgccga ccagcacgat gccttctgcc gcatcgccca
     7981 attctttcag cgcttcgcct actccatccc ctgcctcaac gctaccgctc atgagctgca
     8041 ggcccttgcc gacgtacgct gcggtcagtt tagcggcaac tgcctcggtg gcgtcgacca
     8101 cgggagcgcc gctcttcggt ttcgcgacga gcgtgccgtc ggtctcgaga tagagaccgg
     8161 ttgtgggaat gcggtatgtc ggatcgctca ttgtagctaa tactccactg ctaaagattt
     8221 cgctctgcgc gttccgtacg attaagccgg gctgttcttc gcgaggctcg attcgactat
     8281 aggaaagcga gcctgtcaag tgaaaatagc cggcggagcg gatgaattta cgcagaagct
     8341 tcttaagcgt gccggtagac tgaaatcagc gagctgatga agatctctta tctcgggatt
     8401 tggaattcga gttggcgagt gacctgccta cgatcgtgac acgtttcgaa ccgagtgcag
     8461 cattttactt tctctcgtcg tgagtgtgtg aaacgctgga acaattggac gtttggaaca
     8521 accgagaatt tgacgttgaa cgtttgaaac ttgaactttg aacggctgtt tactattgcg
     8581 gcgacgaaac atctgaatca cgatctggaa gagacgctga ttgtcggcac acgccgcttc
     8641 taccatgccg ttggcgatac aggcaacgcc gaattgacac aagccggtgc tttggtatcg
     8701 t
//
LOCUS       DASUVS010000040        13716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_449710, whole genome shotgun sequence.
ACCESSION   DASUVS010000040 DASUVS010000000
VERSION     DASUVS010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13716
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449710"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VF182_02410"
     CDS             complement(<1..479)
                     /locus_tag="VF182_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal
                     protein"
                     /protein_id="HEX6435955.1"
                     /translation="MKFHFVKTRLFVALIISAIAFDAAMAAAVMAASASQIDRDAAQA
                     LASLYKNVPAAKALGDKSVGVLVFPSIVKGGFIIAGQYGDGALRKNGKSVAYYRSLAA
                     SYGFQAGIQAYGYVLFFMDDESLQYLDNSGGFELGTGPSLVVLDEGFGKNFSTTTLK"
     gene            complement(587..973)
                     /locus_tag="VF182_02415"
     CDS             complement(587..973)
                     /locus_tag="VF182_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435956.1"
                     /translation="MKLIICAAAILLSMQTVSAADFDGSKKLICATTLAMDCVAGREC
                     IKGPPAEIGAPKFLSIDFAQKTVGGPKRSTAIVFMDKGDDQITLQGNELGFGWTIALG
                     QDDGDIVATLVNRDGAFLLFGSCTPL"
     gene            complement(998..1468)
                     /locus_tag="VF182_02420"
     CDS             complement(998..1468)
                     /locus_tag="VF182_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEX6435957.1"
                     /translation="MKTRSLLVIQLVSLALTVPAAAQQPIVYPAKGQSAQQQKKDDAD
                     CYAWAKQTTGIDPAVVASTPPAQQTGPATGGGQRVRGAARGALGGAAIGAIAGDAGTG
                     AGVGAVVGTMAGGRQARQQQAAQNQQSVSQHQDLINTHYRAFSACMEGRGYTIK"
     gene            complement(1519..2001)
                     /locus_tag="VF182_02425"
     CDS             complement(1519..2001)
                     /locus_tag="VF182_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435958.1"
                     /translation="MKPTNLVSVALLALFMGYAAGSWAQDKPASNLEIIHEKLKADKK
                     LIVAKYMELTESEAQKFWPVYDEYQKDLQRSNERLQSLLESYAADYKGKSLTDEKAKK
                     LLDEWIALEQDEGKRRGAFAPKVLQALPVRKAARYLQIENEFRILLRYDLAATVPLVQ
                     "
     gene            complement(2060..2887)
                     /locus_tag="VF182_02430"
     CDS             complement(2060..2887)
                     /locus_tag="VF182_02430"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEX6435959.1"
                     /translation="MKTAIRVTVLVLALMFAGSVKSSMAAGEALIPVEIEAPRVAGAA
                     VGFLPDYEGSDDYTFGIAPAFRYTFQKQERYIQLLANELSFNVLNHPNFRLGPVFTYH
                     FGRNNQIDDRQVKRMKEIDGTIEGGIFADYVFRLSQNPRHRFIVGGLGQYDLGGESHG
                     WKFTGSARYFHPVSTPIDLFVTTQLNFATKDYMDTYFAVDSEDSTRSGLPHYTAGGGA
                     KDWALSGGLLFYLSRSWVTTAAVRYARLFSHAADSPIVDDRGSENQFFGGVGVAYMW"
     gene            complement(3531..4649)
                     /gene="ydiK"
                     /locus_tag="VF182_02435"
     CDS             complement(3531..4649)
                     /gene="ydiK"
                     /locus_tag="VF182_02435"
                     /inference="COORDINATES: protein motif:HMM:NF008216.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HEX6435960.1"
                     /translation="MSADKRIAQLGPSQHDTARNVLAVLFIVALIGSSLWILRPFLGA
                     MVWAAAIVVATWPLMISLQALLWGKRALAVTVMTLLLLCVLVVPLTFAVGTIVSNVDE
                     VVAWAKSLATFTMPALPEWIADLPLVGPRTAELWEGVAASGIQELTAKAAPYAGIFIG
                     WFLAQVGNVGLLLVQLLLTIILAAVMYAKGELVALRVLRFGQRLGGAGGENAVRLAGQ
                     TIRGVALGIVVTALAQTLIGGLGLGIAHVPYAAVLTVAMFLLAIAQIGVVPVLGCAMA
                     WLYWTGESGWGTFMLVWTIIVGGMDNFLRPILIRKGADLPLLLIFAGVVGGLLAFGLI
                     GIFIGPVVLAVADKLLTAWIDRDKLAFEDDERQEPAGE"
     gene            complement(4665..5747)
                     /locus_tag="VF182_02440"
     CDS             complement(4665..5747)
                     /locus_tag="VF182_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435961.1"
                     /translation="MYRKRFSIIALVTIVLSGCASIGPGTVSRDRFDYISAISDSWKS
                     QMLLNLVKLRYGDAPVFLDIASVINQYGIEGSFGFSGNWTQNAQPPWPYGALYGVLGS
                     GRYAERPTITYSPLTGEKFARSLMTPIPPTAILNFLQGGYPVDLVMRLAVHSINGIRS
                     RYGGGARTRDADPEFYTLLEKLRNIQQSGDLGLRVRKTGEQTSTLVVFSKKANAEIAA
                     ARAEVRELLGLDPKAEEFSVVYGSVATSDREIALLTRSMLEILIDLASYIEVPAASTA
                     ERRTFPTPAAEMVNGAPMAPLIRIHSSPENPGDAFAAVPYQRDWYWIDDKDLASKRLF
                     SFIMFLFTLTEPGDRQGAPIITVPVG"
     gene            complement(5851..6918)
                     /locus_tag="VF182_02445"
     CDS             complement(5851..6918)
                     /locus_tag="VF182_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435962.1"
                     /translation="MATDISSEQRLWLFSRRVDFWLASGGAAAALIAAALVIFWHGDR
                     EIDALDFVLSEFHLGATYHAVLQRRLWRRLPFDVLLVPLAIVAITYTLSLNGQTLLLT
                     SIAMCAAIWHRGRQSLGVARFYQRIVGGPASPAHHRLFQGAIYLPMVAAFLAYNHLAP
                     LGYEGEPYLALNLGAEVTSIALIAAAAWVIAYLAWTFCQSRNRRLPIVSQNSGIQLHV
                     GELWVVLAHAVGFGSGYVLGASNISFLLVLAVHHEVQYLYFAYAMSRRLGWSQRATKN
                     ETDRAIGGSCCTALFKREGGFRSELRHAACFIVWPVIGFGGAVGGSWLALAWLAPLGV
                     GGLFCHYWLDGRIWTRRSLPA"
     gene            complement(7103..8314)
                     /locus_tag="VF182_02450"
     CDS             complement(7103..8314)
                     /locus_tag="VF182_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435963.1"
                     /translation="MNQEGSTSNTNLRPEPPDFSLVLGGPLFQLLRRSHLSGDALELW
                     RQRILGITLVAWLPLLALSALEGQALGGRAAVPFLLDVEAHVRFLIALPLLIVAELVV
                     HQRMRLIVRQFLERNLVPGSALPCFDAAIKSAFRLRNSLIAEVLLVALVYIVGVLIFW
                     RHYVALATSTWYALPSGEGLQFSLAGKWYAYVSLPLYQFLLVRWYYRIFIWMRFLWQV
                     SRIQLSLVPTHPDRVGGLGFLANTVYAFMPLAVAHGAMLAGPIANRIFYLGASLPEWK
                     VEIAVLVIFLLCLVIGPLLVFAPQLAQTKRIGNREYGTLAERYVREFDNKWLRGGAPA
                     GEPLVGSADIQSLADIVNSFEVVRTMQIAPITRDAFIRLVVSTLLPLAPLLLTMMPLE
                     ELLRKLLGILF"
     gene            complement(9063..10550)
                     /locus_tag="VF182_02455"
     CDS             complement(9063..10550)
                     /locus_tag="VF182_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012595408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX6435964.1"
                     /translation="MASTTYPSLYQINTRVWLTELSRKHGKRATLDYIPDAELDGIKE
                     MGFDWIWFLSVWQTGPAAQAISRASPEWRKDFEQTLPDLREEDIAGSGFAIQDYTVHR
                     DLGGPAALARLRQRMQQCGLKLMLDFVPNHMAPDHPWADDLPDYFVHGSESDLARAPQ
                     NYCRMQTKHGQLVLAYGRDPYFDGWPDTLQLNYGNPDLQEAMIAELSKIAAQCDGVRC
                     DMAMLILPEVFERTWGIQAESFWPKAIRRVREKFPAFTFMAEVYWDMEWTMQQQGFDY
                     AYDKRLYDRLREGHARPVREHFHAGLDYQNKLARFLENHDEPRAAATFTPEIHRAAAV
                     ITFLSPALRFFHQGQFEARTKRISPHLVRAPDEPVDQSLNEFYDRLLAVLRLPVIRGG
                     EWQLLQPVRAWEGNWTWDCFLAFAWSGSDEERVLVVVNYAPNQSQCYVRLPFSGLRDH
                     RWQLRDLLGDAQYDRDGSDLEARGLYVDMRPWQAQVFSTSKFDKN"
     gene            complement(10645..11025)
                     /locus_tag="VF182_02460"
     CDS             complement(10645..11025)
                     /locus_tag="VF182_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HEX6435965.1"
                     /translation="MNEPNRVTLGVLLLSVLTLYGCEDLVIRDTSSLLAPRFQRSEIV
                     GLVAGFGTTFAAVPDLIAMLKRRSSAGMNPRMAGIMGVFQIAWVYYGLLIVSRPVVVW
                     NIIGVVINFVNVGAYLYFVRKARL"
     gene            complement(11145..>13716)
                     /locus_tag="VF182_02465"
     CDS             complement(11145..>13716)
                     /locus_tag="VF182_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEX6435966.1"
                     /translation="RWGEDGLGGICDEKQRLCFALALWNERDPILKERLFGLTNSEGN
                     HGEDVKEYYFYLDSTPTHSYMKYLYKYPQREYPYRDLVETNRRRSREEFEYELLDTGI
                     FNDDRYFDVFVEYAKESPEDILVRITVHNRGPEAARVRVLPTLWFRNTWSWGEDQSKP
                     SLREVGPGAIRATHHELGDYWLFCEGVPELLFTENDSNAARLWGESNATPYVKDAFHE
                     YLISKRSAAVNPAKLGTKAAAHYILDVPGGGNKTVRLRLVGAELKNPFAGFEQTVKSR
                     IADADEFYERISSNSLNEDERRVHRQALAGMLWSKQYYYFDLDQWLLEHRSHPLMESP
                     RNGGRNAEWFHMLNSDVISMPDKWEYPWYAAWDLAFHTISLSLVDFDFAKEQLLLMLR
                     GLYFHPNGQIPAYEWNFSDVNPPVHAWATLFLYKTEQTLGRADLRFLERSFQGLMLNF
                     NWWVNRKDPAGRNVFAGGFLGLDNIGVFDRSAPLPTGGFLEQADGTAWMAFYCQTMAE
                     MALILTEYDPMYEDMAFKFLQHFMWIAYAMDRIGEHHDEMWDEQDGFFYDLLRLPDGR
                     AMRLKVRSLVGLLPLCASTVFPPKADERFPRLTEMIALFRKRHPELVSHVAPTSDGYI
                     GYKGRRMLSILNKQKLERVLAYLLDENEFLGSHGIRSLSRYHLEHPFVFQVGGQEYKV
                     HYLPGESNTGMFGGNSNWRGPVWMPVNALIIRALLNLYSFYGNEFKVQCPTGSGRYMT
                     LFEVAQEISRRLAGTFLRDPNGRRPVYGGTAKFQDDPHWRDLILFYEYFHGDNGAGLG
                     ASHQTGWTGLIARLIDIFGRIEAKDVELDREQLIDRVVREQVGGEKRDGKEGEAMRDP
                     "
BASE COUNT         3198 a         4035 c         3669 g         2814 t
ORIGIN      
        1 ctcttgaggg ttgtcgtcga aaaattttta ccgaagccct cgtccaggac caccaggctt
       61 ggcccggttc ccaattcgaa tccgccgctg ttgtcaagat attgaagcga ctcgtcgtcc
      121 atgaagaaca acacatagcc atacgcctga atgcccgcct ggaaaccgta cgaagcggcc
      181 agagagcgat agtaggccac gctcttgccg tttttgcgca gggcgccgtc gccatattgg
      241 ccggcaatga tgaagcctcc cttgacgatg ctcgggaata ccagcactcc cactgattta
      301 tcccccaagg ctttggctgc cggaacgttc ttatacaggc tggccaaggc ctgtgcggca
      361 tcgcggtcaa tctggcttgc gctcgcggcc atcacggccg ccgccattgc ggcgtcgaat
      421 gcaattgcgc tgattatcag cgcgacaaat agacgtgttt ttacaaaatg gaatttcatc
      481 atgtctcctt cagtacaact atggagtgcg agcttgccca tgagagtagg tttacaggcc
      541 cttgggcgtg tatgaatgct cgcaaccgtg attgctcgag cacgacctat aacggcgtgc
      601 aagagccgaa caggaggaaa gctccgtcac gattcaccaa ggtggcaact atatcaccgt
      661 cgtcttgacc caaggcaatg gtccagccaa atccaagctc gttgccctga agcgtgatct
      721 ggtcgtcgcc cttgtccata aacacgattg cagtacttcg ctttggccct ccgacagtct
      781 tctgcgcgaa atcgatgctc agaaacttgg gcgcgccgat ttcagccgga ggtcctttga
      841 tacattcccg gccggcaacg cagtccatcg caagcgttgt tgcgcaaatc agttttttcg
      901 atccgtcgaa gtcggctgcg gacacagtct gcatgctcag taatatcgcc gcagcgcaaa
      961 taataagttt cataggtaac ctcccggcgc gctcatctca cttgatggtg tatccgcgac
     1021 cttccatgca tgcgctgaaa gctcggtaat gagtgttgat caagtcttga tgctgagata
     1081 cggattgttg attctgcgcc gcctgctgtt ggcgcgcttg cctgccgccg gccatggtgc
     1141 cgacaaccgc gccaactccg gcccccgtgc ccgcatcgcc ggcaatggcg ccgatggcag
     1201 cgccgccaag ggctccgcgc gcagcgccgc gaactcgctg gccgccgcct gtggctggac
     1261 ccgtctgctg ggctggtgga gtcgatgcga ctaccgctgg atcgatacca gttgtctgct
     1321 ttgcccaagc gtagcaatcc gcatcatctt ttttttgctg ttgagcgctc tggcccttgg
     1381 ctgggtagac aatcggctgt tgggccgccg cgggaactgt caatgccaag cttacaagct
     1441 gaatcacaag taaactccgg gtcttcatgt gtcctcctat agctcgatag ccgtcaccgg
     1501 cacccgtgct tcgatgctct actgcacgag cggcaccgtg gccgccagat cgtagcgcag
     1561 caggatacga aattcgtttt cgatctgcag gtagcgcgcc gcttttctca ccggcaacgc
     1621 ttgcagcact tttggagcaa aagctccccg gcgctttccc tcgtcttgct cgagcgcaat
     1681 ccactcatcc agcagtttct tagccttttc atcggtcagc gacttgcctt tgtaatccgc
     1741 cgcataactc tcgagtaagc tttgcaaccg ctcattgctc ctttgcagat ccttctggta
     1801 ttcgtcgtag accggccaga acttctgcgc ttccgattcc gtcaattcca tgtactttgc
     1861 gacgatcagc tttttatccg ccttgagttt ttcatggata atttccaaat tactggccgg
     1921 cttgtcctgc gcccaggaac cggcggcgta acccatgaac agcgccagca gtgcgacact
     1981 aaccaaatta gtcggtttca tctgtccctc ttatatccat ccgcaaatcg atccctttaa
     2041 gcaaagccca gtcgctttcc taccacatgt aagcgacgcc tactccacca aagaactgat
     2101 tctcggaacc ccgatcatcg acaatcggac tgtcggcagc atgactgaat aaccgagcgt
     2161 accgaaccgc tgcggtggtt acccatgaac ggctcaaata gaacagcagg ccgcccgaga
     2221 gggcccaatc ttttgcgcca ccgccagcgg tgtaatgggg cagtcccgat cgggtcgagt
     2281 cttccgaatc cacagcgaag taggtatcca tgtaatcctt ggttgcaaag ttgagctgag
     2341 tcgtgacaaa aagatcgatg ggagtggata ccgggtgaaa ataacgagct gatccggtaa
     2401 atttccagcc atggctttcg ccgcccaaat cgtattggcc cagcccaccg acgatgaacc
     2461 gatgacgcgg attctgggat agccgaaaaa cgtaatcggc aaaaatgcct ccctcgatcg
     2521 ttccgtcgat ttctttcatg cgcttgactt gtcgatcatc aatctgatta ttccggccaa
     2581 aatgataggt gaaaaccggt cccaatctaa agttcggatg attcaacaca ttgaacgaca
     2641 gctcgttggc cagtagctgt atgtaacgct cctgcttctg gaatgtgtag cggaaggcgg
     2701 gcgcgatgcc gaacgtgtag tcgtctgatc cctcataatc cggtaggaag ccgacggccg
     2761 ctccggccac ccgaggagct tcgatttcca ccggtattag cgcctcgcct gcggccatcg
     2821 aagatttaac cgagcctgcg aacattagcg cgagtacaag gacagttact cgtattgctg
     2881 ttttcatttt cttttccttt ctacgtttca aaacgtttgg cactgttatt tttgttcaaa
     2941 ggagtgagcg ccctattgag gtctgccgcg tcactttgtg gtgtcttctg gatcaccttt
     3001 acggatttgt gggaaacggc aagcgtgttc tcatttcgca ttttgtcatg cggcacgctc
     3061 gaaaaagttt tgcctttcag cttctctctg acaatcccaa atgactcatc ggcttatctc
     3121 cgtctatgga tcgttttgtc ccgaaggaga tgaatggtcg cagtagtagc tctgccaaac
     3181 ccactataac taattgcaac accattcggc ttcgcatcga tctccctatt atcaaaattc
     3241 atggataatc gacacatcta aattatttat gatcaccact gatagcagaa tacccagccg
     3301 gacggatatt ggcctttggt ccaataccaa aacagcttgg atgcgcggaa cacctatcga
     3361 aacgtctctc gcaggtactg gagcaatcgc ggcaaaagcg caagtttcgg tggcgaacgg
     3421 ggcaatggac aggcacgtcg ggagtcgacc aagagtacaa tgaacgacta tgagtttcga
     3481 gggtagcata acgcggggcg aaacagcata tcggcagccg ttctgagttc ttactctccc
     3541 gcgggttcct gccgttcgtc gtcttcgaaa gccagcttgt cgcggtcaat ccacgccgtg
     3601 agcagcttgt ccgccaccgc caataccacc gggccgatga agataccgat gagaccaaag
     3661 gccagaagac cgccgacgac gccggcaaag ataagcagca acggcaagtc cgcgcccttt
     3721 ctgatcagga tcggacgcaa aaaattatcc atgccgccga ctataatcgt ccagaccagc
     3781 atgaatgttc cccaaccgga ttcacccgtc caatagagcc acgccatcgc gcacccaagc
     3841 accggcacca ctccgatttg cgcgatcgcc aaaagaaaca tcgctacggt caacacggcg
     3901 gcgtagggca catgcgcaat ccccaagcca agtccgccaa taagagtttg ggctagtgcg
     3961 gtcaccacaa tgccgagcgc aacgccgcga atcgtctggc cggcgagccg gacggcgttt
     4021 tccccgcccg cccctccgag ccgttgcccg aagcgcagca cgcgcaacgc cacaagctcg
     4081 cccttggcat acataaccgc ggcgagaatg atggttagca aaagctgcac taacaacagt
     4141 ccgacgttgc ccacctgggc aagaaaccat ccaatgaata tcccggcgta gggcgccgct
     4201 ttggcggtga gttcttgtat tccagaggca gcgacacctt cccacagttc tgccgttctc
     4261 gggccgacca acggcaaatc ggcaatccat tcgggtaaag cgggcatcgt gaaggtggcc
     4321 agcgattttg cccaggcaac aacctcatcg acattggaga caatcgtccc aacggcgaag
     4381 gtcagcggta cgaccagcac acacagcagt aagagtgtca tcacagtaac agcgagggcg
     4441 cgtttgcccc acagcaacgc ttgtaatgaa atcatcagcg gccaggtggc cacgacaatc
     4501 gccgccgccc agaccatggc tccgaggaat gggcgcaaga tccagagtga agatccgatt
     4561 agggcgacaa tgaaaagcac ggcgagcacg ttacgcgcag tgtcgtgctg acttggaccg
     4621 agctgcgcta ttcgtttatc ggcactcata atcaggtcgg cacgctatcc gactggaacc
     4681 gttataatcg gggcgccttg tctgtcgccg ggttcagtca gggtgaaaag aaacatgata
     4741 aaagaaaaca gtcgcttcga agcaagatct ttatcatcga tccagtacca gtctcgctga
     4801 taagggacgg cggcaaaggc atccccgggg ttttcaggag agctgtgaat cctgatgagt
     4861 ggcgccatcg gagcgccgtt gaccatctcg gccgccggcg tcggaaaggt gcgccgttcg
     4921 gccgtgctcg ccgccggaac ttcgatgtaa gacgccagat cgatcaaaat ttcgagcatg
     4981 gaccgggtca ggagagcaat ttctctatcg ctcgttgcca cggaaccata aaccacgctg
     5041 aactcctccg ccttgggatc cagccccaag agctcgcgca cttcggcacg agccgccgcg
     5101 atctctgcgt tggccttttt gctgaaaacg accaatgtcg acgtttgctc accggttttc
     5161 cttacccgca acccgagatc gccggattgt tgaatgttcc tcaacttctc cagcaacgtg
     5221 tagaattccg gatcggcatc gcgcgttcgc gccccgccgc cgtacctact gcggatcccg
     5281 ttgatgctat gcacggcgag ccgcattacc agatccaccg gatatccgcc ctgaagaaag
     5341 ttgagaatgg ccgtaggcgg aatcggcgtc atcagactgc gcgcgaactt ttcaccagtc
     5401 aacggagaat acgtaatagt cggccgctcg gcgtatcgtc ccgaacccaa cacgccataa
     5461 agggcgccgt agggccatgg aggctgagcg ttctgcgtcc aattgccgga aaatccgaac
     5521 gacccctcga tgccatattg gttgatcacg gaggcgatgt cgagaaacac cggcgcatcg
     5581 ccgtagcgca gtttgaccag attgagcagc atttgcgatt tccacgaatc agagattgca
     5641 gaaatatagt cgaagcgatc gcgcgagacc gtccccgggc caatgctcgc gcagccgctg
     5701 aggacaatgg tcaccaacgc tatgattgag aatcttttcc gatacattga attccctttt
     5761 gcagttgtca tgtttcacgg gtcaggaaag tcctgaaact cggaacctaa acattccagc
     5821 gagcgaagtt gtcagctgac atcgagattc tcacgcaggc aatgaccgcc gcgtccaaat
     5881 gcggccatcg agccaatagt gacaaaagag cccgccgaca ccgaggggag cgagccatgc
     5941 gagtgccagc caactaccgc caactgctcc gccgaaaccg atcaccggcc acacgataaa
     6001 gcaagccgca tgtctgagct cggatcgaaa accaccttct cttttgaata aagcagtgca
     6061 acaagaaccg ccgattgcgc ggtccgtctc gttcttcgtc gctctctgac tccagccgag
     6121 gcgccgactc atagcatagg cgaagtagag atactgcact tcatgatgaa cggcgagcac
     6181 caacagaaag gagatgtttg acgcgccaag cacataaccg ctgccgaagc ccacggcgtg
     6241 cgcgagaacc acccaaagct cgccgacatg aagctggatg ccgctatttt gcgatacgat
     6301 cggcaaacgc ctattgcggc tttgacaaaa tgtccacgca agatacgcga tcacccaagc
     6361 cgctgccgcg atcaacgcaa tcgaggtaac ttccgcccca agattcagcg ccaagtacgg
     6421 ctctccttca tatcccagcg gcgccagatg attataggca aggaaagccg ccaccatcgg
     6481 cagataaatg gctccctgga acagacgatg atgagccggc gaagccggcc cgcccacgat
     6541 gcgctggtaa aaacgcgcga cgccgaggct ttgccggccg cggtgccaaa tagcggcgca
     6601 catcgcgatg gaggtaagca agagagtttg gccgttcagc gatagcgtat aggtgatggc
     6661 cacgatcgcc aacggcacaa gcagcacatc gaaaggaaga cggcgccaca gccggcgctg
     6721 taaaacggca tggtaggtcg cccccaaatg gaactccgat aacacaaaat cgagcgcatc
     6781 gatttcccga tcgccgtgcc agaaaatgac cagcgctgcc gcaatcaagg cggccgcggc
     6841 tccgcccgag gccagccaaa agtcgacccg acgtgaaaac agccagaggc gctgctccga
     6901 agaaatgtca gttgccatcg acgtcctaat tagaatccga aatcggaaaa tccaaatcag
     6961 aaaaattcac aatacctaat gggcttgaag ttcgtcccag gttgcttcga ggatttagat
     7021 tgtttcgatt agcgattgtc ggctcggagt tttcttactc ctgacgtctc tcgtccccgg
     7081 gatcgtttta gttggcttca tactagaaca aaatcccaag tagcttccgc agcagttctt
     7141 ccagcggcat catcgtgagc agcagcggcg caagcggcaa gagtgtcgag accacaaggc
     7201 gaatgaaagc gtccctggta atcggcgcga tttgcatcgt ccgaaccact tcgaaactat
     7261 taactatatc cgcaagcgac tggatgtcgg cgctgccgac caacggttca ccggccggcg
     7321 cgccgccgcg cagccacttg ttatcgaact cgcgaacgta acgctcagcg agggtgccgt
     7381 actcgcgatt gcctatgcgc ttggtttgag caagctgggg agcgaagacc agcagcggac
     7441 cgatgaccag gcacaggaga aagatgacca agacggcaat ctcgaccttc cactccggca
     7501 aagaggcccc gaggtagaag atacgattgg cgatcggtcc ggcaagcatg gcaccgtgcg
     7561 ccaccgccag cggcatgaag gcatacacgg tgttggcgag gaatcccagg ccaccgacgc
     7621 ggtcgggatg agtcggaacg aggctcaact ggatccgtga aacctgccag agaaagcgca
     7681 tccagataaa tatccgataa taccaacgca ccaggagaaa ctggtagagc ggcaaactga
     7741 cgtacgcata ccactttccg gcaagcgaga actgcaaacc ttcgcctgac ggcaacgcat
     7801 accaggtgga ggtcgcgaga gccacgtaat ggcgccagaa aatcagaact cccacaatgt
     7861 aaaccaacgc gaccagcaag acctcggcga ttagcgagtt gcgcagccga aacgccgact
     7921 tgatcgccgc atcgaagcac ggcagagcgc ttcccggaac tagattacgc tcaagaaatt
     7981 gtctgacgat gagccgcatg cgctgatgca cgacgagttc ggcaacgatc agcaacggca
     8041 aggcgatcag aaatcggacg tgcgcttcca cgtccaaaag aaacggcacc gctgcacggc
     8101 cgcccagcgc ctgtccttcc aaagcggaca gtgcaagcag cggcaaccaa gcgaccagcg
     8161 taataccaag gatgcgttgc cgccataact ccaacgcgtc tcccgagaga tgagagcggc
     8221 gaagaagctg gaacagcggt ccgcccagta cgagcgagaa gtccggcggt tccgggcgca
     8281 agttagtgtt cgacgttgac ccttcttggt tcatatcgcc acaccgcgaa tggcaaccct
     8341 taaattagcc gcgtctcaac ggcgccgatt ggccatggga gcatatcggg ttcgcagcag
     8401 cttcctctat tggactttag tccaatggcg atgacgccat gggaggcgtg aaatcgaacg
     8461 cggaaaaaca aactcaaggc aaaatgctac cgcgtccctc atatcggcgt ttgggcccga
     8521 cactgaagct tgcaggaatt gaagtgccgg ctcgttttcg gctcacggcg cgtcgatagc
     8581 aacataccgg gcgagagagc gctgcttcct gtgatcaggc aaataatcaa cggcgctaga
     8641 atacgctgga gctaaacccg gattccgcag ttcaaaaaga cacggctctc aagcggcgag
     8701 ccctccagag cgaatgcaat gggccagtgg cttggcgcgg cctgtaatcg tcggtgtgca
     8761 gtgaactccg tggaaaaaat tacgagccct ggaccatgca ccgtaggcag cctggattgt
     8821 tcgcttctgt gccttcgctc ctccggactc gccccttgat cagtacacat cgttaaccgc
     8881 tactgcggaa ccggagctga atgacacggt gcgccgaaac atcgccgcaa acggttcgac
     8941 tgccgacttc gatgctccgc gtatgccaat ccaggctgtt ttttgttcac gcaaagacgc
     9001 gctggcccgt caattaaaac aatgctgcgg caaccagggc acagagaagg tcaccgaact
     9061 cattagttct tgtcaaattt cgacgttgaa aaaacctgcg cttgccacgg gcgcatatcc
     9121 acgtacagcc cgcgtgcttc gaggtcgctg ccgtctcggt cgtactgcgc gtcgcccaac
     9181 aagtcccgta gctgccagcg gtgatcccgc aggcccgaga aaggaagccg cacgtagcac
     9241 tggctctggt ttggagcata attcaccact acgagcacgc gttcttcgtc cgaaccggac
     9301 catgcaaacg cgaggaaaca atcccaggtc cagtttccct cccaagcgcg aacaggctgg
     9361 agcagttgcc attcgccacc ccgtataacc ggaaggcgaa gcacggcaag taggcggtca
     9421 tagaactcgt tcaggctttg atcgacgggc tcgtccggcg cgcggaccaa atggggcgag
     9481 atccgttttg ttcgcgcttc gaattgccct tgatgaaaga agcgcagcgc cggagacaga
     9541 aaggtaataa cggccgccgc tcggtggatt tcgggagtaa acgtggccgc cgctcgtggt
     9601 tcgtcgtggt tctcgagaaa tcgagcgagc ttgttctgat aatccaagcc agcgtgaaaa
     9661 tgctcgcgga ccgggcgcgc atgcccttcg cgcagacgat catagagccg tttgtcatag
     9721 gcgtaatcaa agccctgctg ctgcatcgtc cattccatgt cccagtaaac ttcggccatg
     9781 aacgtgaatg caggaaattt ttcgcgcacg cgccggatcg ctttcggcca gaaggactcc
     9841 gcctggatgc cccaagttcg ttcgaagact tccggcagaa tcaacatcgc catatcgcag
     9901 cgcacgccgt cgcattgagc cgcgatcttc gatagctcgg cgatcatcgc ttcctgcaga
     9961 tcagggttgc cgtagttcag ctgcagagta tcgggccagc cgtcaaaata tgggtcgcgc
    10021 ccgtaggcga gcaccagctg gccgtgtttc gtttgcatgc ggcagtagtt ctgcggtgcg
    10081 cgagccaaat cactctcgct gccatggaca aagtagtccg gaaggtcgtc ggcccacggg
    10141 tgatccgggg ccatgtggtt cggaacaaaa tcgagcatca acttcagtcc gcattgctgc
    10201 atccgctgtc gcaaccgtgc gagcgccgcg ggcccaccca gatcgcggtg tacggtgtag
    10261 tcttgaatag cgaatccgga gcccgcgatg tcttcttctc gcaagtccgg cagcgtctgt
    10321 tcaaagtcct tgcgccactc gggactcgcg cgcgagatcg cctgcgccgc tggtccggtc
    10381 tgccacacgc tcaagaacca gatccaatca aatcccatct ctttaatgcc atccaattca
    10441 gcgtcaggaa tatagtccaa ggttgcccgc ttaccgtgtt tccgggaaag ctcggtcagc
    10501 caaacgcggg tgttgatctg gtagagcgaa ggataggttg ttgatgccac aattggtatt
    10561 gctcaaagat tgctactgct tccagacact gtccgggaaa gactgaatag ataacgtcgg
    10621 cgaacgggct tgttcactgg ccgcttacag ccttgctttg cgaacaaaat atagatacgc
    10681 gccgacattc acgaaattga tcactacacc gattatgttc cagacgacca ccggccgtga
    10741 aacaatcaac aaaccgtagt aaacccaggc gatttgaaaa acgcccatga tgcccgccat
    10801 cctcgggttc atcccagcgc ttgagcgccg cttgagcatt gcgatcaagt cgggtacggc
    10861 ggcaaaggtc gttccaaatc cggccaccaa gccaacgatc tcagaccgct gaaaccgggg
    10921 ggcaagcaag ctcgaggtgt cgcgtatcac caggtcctca cagccataaa gtgtaagaac
    10981 gctcagcaag agaacaccaa gcgtcactct gttgggctca ttcatattca cttccgcaac
    11041 ttctatagcg gctcctaatt accttgtgct ctcaggctct gcctgctcat aagtttgcag
    11101 acaggttcga acgataagca ttgatgttca tctgacatgg ggtttcacgg atcgcgcatt
    11161 gcctctcctt ccttcccgtc cctcttttct ccgcccactt gctcgcgcac tacccggtcg
    11221 ataagttgtt cgcggtccaa ctccacatct ttcgcttcga tgcgtccgaa gatatcgatg
    11281 agacgcgcga tcaagccggt ccatccggtc tggtggctgg cgccaagacc cgcgccgttg
    11341 tcgccgtgga agtattcgta aaatagaatc agatcgcgcc agtgcggatc gtcctgaaac
    11401 ttagccgttc caccgtaaac gggtcggcgt ccatttgggt cgcgcaggaa agtgcccgcc
    11461 agacgacgcg agatttcctg cgccacttca aatagcgtca tgtaccgccc cgaaccggtc
    11521 ggacactgaa ctttgaattc attgccgtag aaactgtaca ggttcaacag cgcccgaatg
    11581 atcagcgcat taaccggcat ccacacgggg ccgcgccaat tggaattgcc gccgaacatg
    11641 ccggtatttg attcgcccgg caggtagtga accttgtatt cctgaccccc gacctgaaac
    11701 acaaacggat gttccaggtg atagcgcgat agagaacgga tgccatgaga gccgagaaac
    11761 tcattctcgt cgagcaggta ggcgagcacg cgctcgagct tctgcttgtt gaggatcgac
    11821 agcatacgcc gccctttgta gccgatgtaa ccatcgctgg tcggggccac atgggacacc
    11881 aactcgggat ggcgtttgcg gaacagggcg atcatctccg tcagccttgg gaaacgttca
    11941 tcggccttgg gcgggaacac cgtagacgca cagagcggca gcaatcctac caacgaacgt
    12001 accttcaagc gcattgcccg cccgtcgggc agccgcaata aatcgtagaa gaagccgtct
    12061 tgttcgtccc acatctcgtc gtgatgctcg ccgatgcggt ccatcgcgta agcgatccac
    12121 ataaaatgtt ggaggaactt aaaggccata tcttcgtaca tgggatcgta ttcggtcaga
    12181 atgagggcca tctccgccat ggtttggcag taaaacgcca tccaggccgt cccgtccgcc
    12241 tgctccaaaa agccgcccgt gggaagtggg gcgctgcgat cgaagacacc aatgttgtcc
    12301 aatcccagaa agccgccggc gaagacattg cgcccggcgg gatccttacg attcacccac
    12361 cagttgaagt tcagcatcag cccctggaac gatcgttcca gaaaacgcaa atctgcacgg
    12421 ccgagggtct gctcggtctt gtagagaaac agagttgccc aggcgtgcac cggcggattt
    12481 acatcgctga agttccactc gtaggcggga atttgtccgt tggggtgaaa atagagaccg
    12541 cgcagcatca gcagcagctg ctccttggcg aaatcgaagt ccaccaggga aagcgaaatc
    12601 gtgtggaaag ccaggtccca ggccgcatac caggggtact cccacttgtc gggcattgag
    12661 atcacatcgc tgttgagcat gtgaaaccat tccgcattgc gcccgccgtt acggggcgat
    12721 tccatgagcg gatgactcct gtgctcgagc agccattgat ccagatcgaa gtagtagtac
    12781 tgcttggacc agagcattcc cgccagcgcc tgacggtgca cccgtcgctc gtcttcgttc
    12841 aaagagttcg atgaaatgcg ctcgtagaat tcgtcggcat cggcaatccg gcttttgact
    12901 gtttgctcga agccggcaaa cggattcttg agctcggcgc caacgaggcg cagccggaca
    12961 gtcttgtttc caccgccggg cacgtcgagt atatagtgag ccgcagcctt ggtaccgagc
    13021 ttagcgggat tgaccgccgc actacgtttc gagataaggt actcgtggaa ggcatccttg
    13081 acgtagggcg tagcgttgga ctcgccccaa agacgggccg cattactgtc attctcagtg
    13141 aagagcaact ccgggacgcc ctcgcaaaac agccagtaat cgccaagctc atggtgggta
    13201 gcgcgaatcg cgccgggccc cacttcccgt aacgacggct tcgactgatc ctcgccccac
    13261 gaccaggtgt tgcggaacca gagtgtcggc aggacacgaa ctcgcgccgc ttcaggcccg
    13321 cgattatgca ccgtaatgcg gacgagaatg tcttcggggc tctccttggc gtattccacg
    13381 aagacgtcga agtaccggtc gtcgttgaat atgccggtat caagcagctc gtactcaaat
    13441 tcttcacgcg accgtcttcg gttggtctcg accaggtccc tgtacggata ctcgcgctgc
    13501 gggtacttgt ataggtattt catgtacgaa tgagtgggcg tgctatcgag gtaaaagtaa
    13561 tactctttga cgtcctcgcc gtggtttccc tcgctgttgg tgagcccgaa caagcgctct
    13621 ttgaggatcg gatcccgctc gttccacaaa gccagcgcaa aacacagccg ttgcttttca
    13681 tcgcagattc cgccgagccc gtcctcgccc caccga
//
LOCUS       DASUVS010000041        18855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_13614, whole genome shotgun sequence.
ACCESSION   DASUVS010000041 DASUVS010000000
VERSION     DASUVS010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 18855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18855
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13614"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="VF182_02470"
     CDS             complement(<1..1520)
                     /locus_tag="VF182_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX6435967.1"
                     /translation="MTHYTFPQLLRHDFAGYNIPKFRQDLLAGLTVAAVALPLALAFG
                     VASGANASAGLITAILAGLIIGGLSGAPYQISGPTGAMSAVLIVLAEKHGLSGIWFAG
                     MLAGIFLLVIGMARLGRFIAFVPAPVIAGFTSGIALIIAIGQIDNLLGVQSAPASSAA
                     LKLFGYFTTSLNPDWHALTLGAVVILMMVFWPKSWSVRFPGSLSGIILATVVNAAFGW
                     PVGVIGDIPKTLLLEQRLTWDLIPWAQLQVFVAPALAITALGAVESLLCGAVASNMTG
                     IRLQANQELIAQGIGNAIIPFFGGVPATAAIARSSVGIKSGGRTRMVSIVHALGLLAS
                     MFLLGPIMATIPLAALAGVLMVTAWHMNEWEAIHYIFTHRFKTAMITFLVTMVATVVL
                     DLTEAILLGAFISGAIFLNQSASIDIDVQEVDPEKLRKRGIENAGTCRHVRVAYLTGP
                     LFFAATSNFNEAFARFERTHALILSMRGVPLIDTSGLQAMTALFYKLNKQGITLSVA"
     gene            complement(1785..3074)
                     /locus_tag="VF182_02475"
     CDS             complement(1785..3074)
                     /locus_tag="VF182_02475"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015176220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX6435968.1"
                     /translation="MKRVLAIILGGGAGTRLYPLTKLRAKPAVPLAGKYRLIDIPISN
                     CINSEIQRIYVLTQFNSASLNRHITRTYGMAGFQQGFVEVLAAQQTPENPNWFEGTAD
                     AVRKYCWLFAEWDVDQYLILSGDHLYRMNYADFIRRHRETRADVTLAVIPVEEHGASD
                     FGLMKIDREGRVQSFSEKPKGEGLKAMQVDTGALGLAADKASTLPYIASMGIYVFERQ
                     VLFDLLQESPDRTDFGKEILPAALKNHNVQAYLFKDYWEDIGTIEAFYNANLALTQQP
                     SPPFSFYDEKAPIYTRSRYLPPTKLLDCNVVESMVGEGCILRECTVKRSVIGIRSRIE
                     TGCVIDNVLLMGSDFYESPQERLQTVESGKVPLGIGANTTIRRAIIDKNARIGRNVRM
                     VNQDHVQEGNREDLGFVIRNGIIVIIKNATIPDGTII"
     gene            complement(3360..4226)
                     /locus_tag="VF182_02480"
     CDS             complement(3360..4226)
                     /locus_tag="VF182_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6435969.1"
                     /translation="MIYLLLAIIGSGLIPVLFRGFADWRVNVFWAIPINYLTCVIAGG
                     VWGGHPLALSTLASAPWLWLAMLQGGILAINFFLLAYTAQRVGVSVAALASRLSVAIP
                     SILAFLFYGDSFSAAKIAGLAAALLSLYLCTASTEKSATTRPLGLKLLPLLVFLTFGC
                     YFIVIKYAQSHYLSEASFHDYVMAGFLFGFLTALAICSARRLLIFKQFRLVHVVAGLI
                     LGVVNYVAVYTLIKALALEGWESSQLYPMYSVGVVAVSTGLAMLLFNEKLSNIRKVGL
                     VVGVIAVALLNR"
     gene            complement(4448..5797)
                     /locus_tag="VF182_02485"
     CDS             complement(4448..5797)
                     /locus_tag="VF182_02485"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6435970.1"
                     /translation="MSELIITNALIVPMTQEKRSFHGYAQVKDGVITAVAAGAPVAVK
                     SDELIDADGCVLLPGLVNAHTHLYQVLLRAVWEDLPLMPWLKRIYGAARVLRPEHFYT
                     GSLLGCVEAIRSGVTTVCEHNFLNPHPECAFESVRAIQDSGLRSIFARTIMDTGEIVP
                     DCTRETPENAFARIEAVLARHKNQERLGFMTGPNTPPINTTPDLLKEIRQFADAAGIG
                     ISAHVAESKSVLECAQNEHGAKGVVEFLHRFGIPGNNSIFAHAVHISEEEIAILKQTG
                     TSVSHNPVSNMMLGDGVAPVVEMLRQGVNVALGTDGAASNHSQDLFDTMKAASLLQKV
                     HHQDAGIIEPYAVLRMATAGGAAALGLASLCGTVEVGKRADLILVDLETTHVQPINDL
                     FSQIVHCAKASDVRTVIVDGEILMRNRQLKRHDAEKLLSDAKHGNRDLMDRVNQLSF"
     gene            6129..6536
                     /locus_tag="VF182_02490"
     CDS             6129..6536
                     /locus_tag="VF182_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6435971.1"
                     /translation="MSQETKVFKYRTPQFDGVKKTLQVCNSDLMKVQVQVVKDGGENN
                     LHSHTGDDAFWYVISGAVKFYGEGDEVIGEFNKGEGILIPRGFKYWFESSSSEPLEIL
                     RVTAKDQKVENQRVDHSAQKQWMVDQNTFGTRT"
     gene            6601..6918
                     /locus_tag="VF182_02495"
     CDS             6601..6918
                     /locus_tag="VF182_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435972.1"
                     /translation="MLGDFGAFCVICDNFTGQCFWVRRLLFPPSMEFICQHCDEVATG
                     EKYRVISEENGLVFLNMVVCRSCQQQAKQLGLHAERITYSDRSHSRHSPPADRLMVGH
                     SHS"
     gene            complement(7060..7938)
                     /locus_tag="VF182_02500"
     CDS             complement(7060..7938)
                     /locus_tag="VF182_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX6435973.1"
                     /translation="MKDIEASHMGTKFFYWIVTALAIYLFPAGLIAAEKERPIDVIED
                     NSFLIEEAYNQEPGVVQHIFTAQYVNDSRRRGWEFNFTQEWPIFSQNHQFSYTIPSYH
                     LVETGDRQNGLGDVLLNYRYQLLKEEESELAFAPRFSLILPSGSRRRGTGHGVVGYEW
                     GLPFSKKINGQFALHGNFGLSYLPKARAPLESGGLSSKHSLVSYNLGASAIYAVSRRV
                     HLMLEWLGDFEESIDDNGGTRRTFEPVLSPGVRAAIVDQPMLQIIAGVAAPIGLNRKA
                     DNYGALMYLSIEHDLF"
     gene            complement(8050..9054)
                     /locus_tag="VF182_02505"
     CDS             complement(8050..9054)
                     /locus_tag="VF182_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435974.1"
                     /translation="MNRIKIGSGLVIAALLILRWSPPFAHGAELEKLNVCYSSIAATS
                     VSTWIPKEAGIYQKYGLDVNTVYVSGAQAISTLVSGDTQIVQGSGAAAVLSRLSGADV
                     TIIGTTINVVPMSLVTTPDIARVQDLKGKTFGVSRFGSLTDIGLRKAVSEFGLDAATD
                     IKMIQTGGVPEILLFMQQGVIKGGLISSPTLERAKELGYKELLNLGEIKYRYPGTALV
                     TTGAFIKSRPQTLNRFLKATLEGIKYAKANPDFTVQVLGKYTRNTDTRLLNSAFKSYV
                     LGYIRNVPTITQPEMESVLEDIALRNPKAKGADPKQFYDLAPLEQLIKEGFVKELYPG
                     "
     gene            complement(9363..9563)
                     /locus_tag="VF182_02510"
     CDS             complement(9363..9563)
                     /locus_tag="VF182_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435975.1"
                     /translation="METSETAEYIQISCENSTYNAAVCHRCGTKIFPVELLDAHLDRH
                     QIKDLYLEGELKKLQFAMARMR"
     gene            9674..10918
                     /locus_tag="VF182_02515"
     CDS             9674..10918
                     /locus_tag="VF182_02515"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF004127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6435976.1"
                     /translation="MNQEKLEDAETQRLQLSSNVHSTSTIDIFVLPFDSKLMIDPSPI
                     PSDNVVFSETQAGRFTLGYIKLNNPRALNALTLAMFREIEATLLRWRAREDLVCVVLH
                     SDSEKAFCAGGDVKALVSSLQAGSDLDSGAQYFTAEYLVDYLIHIYSKPLLCWADGIA
                     MGGGIGIMNGASYRAVTERTLMAMPEITIGLFPDVGGTYFLNRLPENLGLFLALTGAR
                     FDGRDAVAIGMADGVIASDKKSQALDGLGNLEWKSDAAYDKEILRRHLSRFAESPTSS
                     ESAVMQRLETIRSLTEQSSIEAVDAALRGWFGKDEWIKSAIAGYFSGSPTSAKAIFRQ
                     INEGKKLNLKQVFLREWDMALNFCARSDFREGVRARLIDKDQKPQWRPPVLGQVQEAE
                     VERLFAKQHGQPDLLSQRFAEL"
     gene            11053..12057
                     /locus_tag="VF182_02520"
     CDS             11053..12057
                     /locus_tag="VF182_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6435977.1"
                     /translation="MWLSALLVGLLMLFSRETGFSQDAKLIEAAKKEGGKVIVYTSME
                     TFTADAIKAAFEKKTGLQMEYWRGGSTEVLDRSLAEFRVGKPLFDVVAMTGDHMHLLA
                     KEGVFVKYQSPSFKGFAKDAIDPNLGARYRNVLYGVIYNKANIKAAEAPKTLEDVVKP
                     EYRGKLVMPHPVNHTLTTQWLASLDKIMPKPRAEKFIRDLAAAKPIFVESIVPAADRV
                     GTGETPIGITFARFVLTYNKQGANLDYVRDYNMLGDGQYISLGSKAPRPNAGKAFIDF
                     FLDEESMTIQAKTGEFVNRQGIYPPLADADKIKFVQMYPFSKEDYEIKKKEYQKIFLQ
                     "
     gene            12169..13242
                     /locus_tag="VF182_02525"
     CDS             12169..13242
                     /locus_tag="VF182_02525"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6435978.1"
                     /translation="MADLVIDADGHIMEQHKDLFDHIKGNFGEMNWHSSWPLFDADGW
                     QRGLARKGKREDPDAEAWIRFQNEHGIDMAVLYPTSALGLGMIQLPAWASALAQGYND
                     WLYHRFTSQTPRLKGVALLAPQDPKAAAAELRRCVKDLGFCAGLLPSVTNNRTPLYGS
                     PLFHEIYDEAERLNVPLTIHGGVSQNLGLDRVASFLEAHMLEHPVGLFLQITNMVFQG
                     VFQEFPKLRIAYLEAGAGWVPFMMDRMEEDYEKFAARLAPNLKSPPSTFFKSGNIFVT
                     MEVEERTAPYIPELVHPDVIMWASDFPHERERDQFGGDLPHLRARKDLSDEFKNKMLF
                     DNPVRFYRLSEGDIAAARKAKGN"
     gene            complement(13361..14350)
                     /locus_tag="VF182_02530"
     CDS             complement(13361..14350)
                     /locus_tag="VF182_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435979.1"
                     /translation="MRRLVAVVFLSLFAMSSVVRGIARAAEPIRIAYSSVNPHALLVW
                     IAEKRGLYAKHGLSSTLVYVPGGSVAIQALVSGDIDLAQLTGPPGVIANMKGADVVYI
                     AMTDDKMGYQLVSRQEVKKPAELKGKRLGISRFGSSSDFSTKVLLRKIGVDPKDVTIL
                     QIGNETQRLVALKSGGIDGSIFNAPFGAEAKKLNFNILADAGALNIPYFNTGMCGSTQ
                     MMQKNEARMLSFLRAYLEAIKIFKTEPEYTLKALSQFSKVNDQELLKEAYEYNRARIP
                     DVPYPSLVAMQAVVETLADSEPKAVKADPRKFINDRFLKKLEDEGFVQKLFGR"
     gene            complement(14368..15411)
                     /locus_tag="VF182_02535"
     CDS             complement(14368..15411)
                     /locus_tag="VF182_02535"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6435980.1"
                     /translation="MAHFPVIDTDGHVLERQSDIRKYLEPPWNKRPTPLWPGDQPWDN
                     DMMDSFEMASQWKALSAAEQVERWHAIMDEHDIEKAICFPTGSGNIAKNQEVPYQIAV
                     ARASNTHFAKEYNDRSDRLSCVGCLPMRLPAAAAAELTRAVEELGLKGFEVLPTGLPL
                     ALGDSFYDPIYAQAEKLGAVLGIHGTRNTSRELGTSGLATFSEVHSYAFPVGILLQFT
                     SMVCQGLPIRFPKLKLAFLEIGATWLPYYLDRLDEHWEKRGKYEMPQLKNKPSDIVRQ
                     SKMYFSVEPGESQLAATVNYVGAEHFLYASDIPHWDNEFPENLIDLRNHRDLSEEVKR
                     KILHDNAKELFAL"
     gene            15811..16281
                     /locus_tag="VF182_02540"
     CDS             15811..16281
                     /locus_tag="VF182_02540"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6435981.1"
                     /translation="MGLHVKSSDVTEIKRVQSGEAGTGSMVVRKGYGNECSLMIATRA
                     PGYHTKPHQHESEQINYILEGEIWFFVEDKGFHCKKGDFQRIPANTVHWAWNRSENEA
                     MVAEAHAPALIGGRAGEYAVGLFDDGEKPQVRGPGVNQFVPFDQQKAEAKYASK"
     gene            16316..17269
                     /locus_tag="VF182_02545"
     CDS             16316..17269
                     /locus_tag="VF182_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435982.1"
                     /translation="MSFLCGLLSIQKLSWAQDLRRIHYGTTNSTSHLPVWVAKDAGYF
                     AKNGLNVEPVHIRGGALITMGIMSGQLQFSGAGAESVVAARIEGGDVVLLACPVYSDP
                     VYLMARPEIKSAAELKGKSTAVTRLGSTTHFYLRAALRHVGLDSEKDLTILQLGAGPE
                     IVSALETGRIAAAALTTRYALPYLERGWPMLVDLSTTDLVYPSSCVASSRNFVKTEPQ
                     VVENFLKAYVDAIQLIVKDPPSAEKSFIRWLRERDPMVIKKSVNAYVRLFKTKPYVPD
                     KGIENVLTDLATRRAIPKEFIGRPELFRDNAPLEKALSSRP"
     gene            17266..18243
                     /locus_tag="VF182_02550"
     CDS             17266..18243
                     /locus_tag="VF182_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435983.1"
                     /translation="MRYQSFIVLLTLVCAFFRTYQAVDAQELRRILYGTSASPSHLPV
                     WVAKDAGYFEKYGFNVEPVQIRGGSLITLAIITGSLQFSGAGAESVIAARAAGADVIL
                     LACPVDVDPVYLITRPEIKTAAELKGHASAVTRYGSTTHFYLRTALRHVGLNPDRDMT
                     ILQLGAGPEMVAALETGRIAAAALTARYAIPFLQRNWPVLVDLSATDLIYPSSCVASS
                     RAFMRAEPKTTEDFLRAYVAGIQLIKRDHAFAEKSLSKWLRENDPVILKKSIQAYAKL
                     FKRAPFVSDKGIDNVVKDLVSMRPEFKEYIGWPEPFRENGPLQSALGRR"
     gene            complement(18245..18613)
                     /locus_tag="VF182_02555"
     CDS             complement(18245..18613)
                     /locus_tag="VF182_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6435984.1"
                     /translation="MGFRVNHLHLKTPDPRKTADFYVEFAGAKIVSERAGPDGRKTFR
                     LDLHGVPLNITDYLSDQKLEQHYGLEHVAIDADNFAAEVAKIKAAGIKILEERVLPDG
                     RHVCFFEGPEGVRLEFLEMR"
BASE COUNT         4511 a         5174 c         4945 g         4225 t
ORIGIN      
        1 gccacgctta gagtgatgcc ttgcttgttg agcttataaa ataacgccgt catggcttgc
       61 aggccggaag tgtcgatcaa aggcaccccg cgcatcgaaa gaataagcgc atgagtgcgt
      121 tcgaatcggg caaaggcttc gttgaaattg ctggtagccg cgaagaacag aggcccggtt
      181 aggtacgcga ctctaacatg ccggcaagtg ccggcgttct cgatgcctcg cttgcgcaac
      241 ttctccgggt ccacttcttg aacatcgata tcgatgctcg cgctctgatt taaaaatatc
      301 gcgcccgata taaacgctcc aagtaaaatc gcttccgtca aatccagaac aaccgtggcc
      361 accatggtga ccaggaacgt aatcatggcg gttttaaaac ggtgagtgaa gatatagtga
      421 atcgcttccc attcattcat gtgccatgcc gtgaccatca ggacgccggc gagggcggct
      481 agcggaatgg ttgccataat cggtccgagc aaaaacatgg aggccaatag ccctagggcg
      541 tgcacaatgc tgaccatccg cgtccggccg ccggacttga ttccaacact actgcgggca
      601 atggccgcag tagccggcac gccgccgaaa aatgggatga ttgcgttgcc gatgccttgc
      661 gcaatcagtt cctgattagc ctgcaaacga attcccgtca tgttgctcgc cactgcgccg
      721 cacagcaaac tctccaccgc gcccaatgcg gtaatcgcca gagccggcgc gacaaacact
      781 tgtagttgag cccaggggat taggtcccat gtcaaccgtt gttcgagcaa cagagttttt
      841 ggaatatcgc ctatgacccc gaccggccac ccgaacgccg cattgacaac tgttgcgagg
      901 atgattcccg agagagaccc cggaaagcga acgctccaag actttggcca gaagaccatc
      961 atgaggatca ccacggcccc gagcgtgaga gcatgccaat ccggattcag gctggtagtg
     1021 aaataaccaa acagctttaa agccgcagaa ctcgcgggcg ctgattgcac gccgagaaga
     1081 ttatcgattt gcccgattgc aatgatgagc gcaatgccgg aggtaaagcc ggcgatgacg
     1141 ggggcgggaa caaatgcgat aaagcgacct aaccgggcca taccgatgac caacaagaag
     1201 atgccggcca gcatcccggc aaaccagatc cctgataacc cgtgtttttc cgctaatacg
     1261 atcagcaccg cgctcatcgc ccccgtcgga cctgaaatct gatagggagc ccctgaaagg
     1321 ccgccaatga tcaagcctgc caatatcgcg gtgatcaaac ccgcgctggc attcgcacct
     1381 gacgcgacac cgaacgccag cgccagtggc aatgcgactg cagcgactgt caagccagca
     1441 agtagatcct ggcggaattt tggaatgtta taaccggcga aatcatgcct gaggagttga
     1501 ggaaatgtat agtgcgtcat ctgatgatgc cttaaaatgc ctcctgttgc ggagcccgag
     1561 ttcgcacgtg cgctcgcgcg cggcaaacca gcggttatat tgttgaatag atgattggat
     1621 gggtcgctag ccgtttacgt ggctgatatt ataggcgcgc ccgagccact gatcaaggtt
     1681 gctctacgaa tttcgcacac gccggaaagt ttatactaaa ggaaaatagt ggaatgaaaa
     1741 tgactttctg cttgtccggg gaaatctata ataatctgcg gcggctagat aatcgtgccg
     1801 tcaggaatcg ttgcattctt gatgatcacg ataataccgt tgcgaatgac gaagccaaga
     1861 tcctcgcgat taccttcctg cacgtgatcc tggttgacca tccggacgtt ccggccgata
     1921 cgcgcgtttt tatcgatgat ggctcgtcga atggtcgtgt tggctccaat ccccaacggc
     1981 acctttccac tctccaccgt ctgtaaccgt tcttgcggcg attcgtagaa gtctgatccc
     2041 attaacaaaa cattgtcgat gacgcagccg gtttcgatgc gcgatcgaat tccgattacc
     2101 gagcgcttta ccgtacattc ccgaaggatg cagccctcgc cgaccatgga ctcgacaacg
     2161 ttgcaatcga gcaacttcgt cggcggcaga tagcgcgacc gagtatagat gggcgccttt
     2221 tcatcataaa agctgaatgg cgggctcggc tgctgcgtga gcgccagatt cgcgttgtaa
     2281 aaggcttcga tggttccaat atcttcccaa taatctttaa agagataggc ctgcacgttg
     2341 tgatttttca gagcagccgg aaggatttcc ttgccgaaat ccgtccgatc aggggattct
     2401 tgcagcagat cgaacagaac ctgcctctcg aacacataaa tccccattga agcaatgtag
     2461 ggaagcgtgc tggctttatc cgccgcgagc ccgagcgcgc cggtgtcaac ctgcatcgcc
     2521 ttgagaccct cccctttggg cttttcgcta aaagactgca cacgtccttc cctgtcgatc
     2581 ttcatcaaac cgaagtccga ggccccatgt tcctccacag ggataacggc aagagtcacg
     2641 tcggctctag tttcgcgatg gcgacgaatg aaatccgcgt aattcatccg ataaagatga
     2701 tcgcctgata aaatcaggta ctgatcgaca tcccattcgg caaataacca gcaatatttc
     2761 cgcaccgcgt ccgcggtgcc ctcgaaccag tttgggtttt ccggcgtctg ctgcgcagcc
     2821 aagacttcga cgaaaccttg ctggaatccg gccatgccat aggttcgcgt aatatgccgg
     2881 ttgagcgagg ccgaattgaa ctgggttaat acatagatcc gttggatctc cgagttgatg
     2941 cagttgctga tggggatgtc gatcaagcga tacttgccgg caagaggaac ggccggcttg
     3001 gcgcgaagtt ttgtcaaagg atacagccgg gttccggcgc ctccgcccaa gatgatggct
     3061 aatactcttt tcacgaggaa ctcatccttg ccttacacta gtcgcaaatg ctctcatgtc
     3121 aaccattcca acccgataaa gattacttta gccaattcga accaattctc tcatgacgcg
     3181 cacagccgat cgatgaaatc gctttttatc gatcgcatgc actaagcggg cggtcagcaa
     3241 atcttcccgg ttcaccgccc gagctttatt ttcttcaacg cgaactccct caacggcgaa
     3301 ccgtacgccg ccgcttaggg atttaccggt ggaccgccac agcactgaag cgccccagat
     3361 caacggttga gcagcgccac cgcgatcaca cccacaacca atccgacttt tctgatgttc
     3421 gataattttt cattgaaaag cagcatcgcg agtccggtgc tcacagcgac cactccgacg
     3481 ctgtacatcg ggtatagctg agagctttcc caaccttcca gcgctagcgc cttaatcagg
     3541 gtgtaaactg ccacataatt caccaccccg agaatcagcc cggcgacaac atgaaccaga
     3601 cggaattgtt tgaaaatgag caagcggcgc gcggaacaga tcgccagagc ggtcaaaaag
     3661 ccaaaaagaa aacccgccat cacataatca tgaaacgatg cctcactcag atagtgggat
     3721 tgggcgtact tgatgacaat gaaataacaa ccgaaggtca gaaatactaa cagcggaaga
     3781 agcttcagcc caagcgggcg cgttgtcgcc gacttttctg tcgatgcggt gcacaaatac
     3841 agagacagca gcgccgctgc caagccggcg attttcgcgg cactaaatga gtcgccataa
     3901 aataggaatg ccaaaatcga aggtatggcc accgacagac ggctcgccag ggcggcgacc
     3961 gaaacaccga ctcgctgcgc ggtgtacgcc agcaggaaaa aattgattgc cagtatgcca
     4021 ccctggagca tcgccagcca aagccatggc gcgcttgcca acgtgcttag agctaatgga
     4081 tggccgcccc agacgccgcc ggcgatcacg catgtcaaat agttgatcgg aatcgcccag
     4141 aacacgttga cccgccagtc ggcaaagccg cggaacagta cggggatcaa acccgatccg
     4201 ataatcgcca agagcaaata aatcatcgtc tagagtacgc cacggaaaac tctcatgtgc
     4261 ttggttaaac tatcggctcg cgtcggtgta tcgggtagcg tgttcgcctg gggattaatc
     4321 cggatgacgt ttcttcactg acaattatga cttctcagga ggcgcgccga tggttcagag
     4381 tagcaatttt cgcgtcggtt tcagacctgg cggcctcgct ttccttagtg tcgcgaaggc
     4441 agcgcaccta gaacgacaac tggttcacac ggtccatcaa gtcacggttg ccgtgcttgg
     4501 catcggacaa taacttttcc gcatcgtgac gcttgagttg gcgattgcgc atcagtatct
     4561 caccgtcgac gatcacggtc cgcacgtcac ttgccttagc acagtgaacg atctggctga
     4621 aaagatcatt gatcggctgg acatgagtag tttccaaatc gaccagaatc aaatcggcgc
     4681 gcttgccgac ctcgactgtg ccacacagcg aggctagacc aagagcggca gcgccacccg
     4741 cagtcgccat tcgcaacacc gcatacggct cgattatgcc ggcgtcttga tgatgaacct
     4801 tttgcaacag cgacgcggcc ttcatagtgt cgaacaggtc ttgcgaatga ttgctggccg
     4861 ccccatccgt gcccagcgcg acattgacac cctggcgcaa catctccacg accggtgcga
     4921 caccatcacc gagcatcata ttgctcaccg gattgtgcga taccgaggtg ccggtttgct
     4981 tgagtatggc gatctcttct tcgctgatgt gaacggcatg agcgaaaatc gaattatttc
     5041 cgggaatgcc gaagcgatgc aggaactcca ccaccccttt tgcaccgtgt tcgttctgcg
     5101 cgcattccaa caccgacttt gactcggcga catgggcgct gataccgatc ccggccgcat
     5161 ctgcgaattg tcggatctct ttgagcagat ccggcgtcgt attgatcggc ggtgtattgg
     5221 gcccggtcat aaaaccgagc ctctcttggt ttttatggcg cgcaagcacg gcttcgattc
     5281 gggcgaatgc attttccggt gtctcgcgcg tgcagtccgg cacaatttct ccagtgtcca
     5341 taatcgtccg ggcaaaaatt gaccgcagcc ccgaatcttg aatcgcccgc accgattcga
     5401 acgcgcactc cggatgggga ttaaggaaat tatgttcaca aaccgtagtc acgccgctgc
     5461 gaatcgcctc aacgcaaccc agcagcgaac cggtatagaa atgttccggc cgtaacactc
     5521 gcgcagcgcc ataaatacgc ttcagccaag gcatcagcgg cagatcttcc cagactgccc
     5581 gcaacaacac ttgataaaga tgggtatggg cgttgacgag accaggaagg agaacgcagc
     5641 catcggcgtc gattagctca tctgatttta ccgcaacagg cgctcccgcg gcgacggcgg
     5701 ttataacccc gtctttcacc tgcgcatagc catgaaatga cctcttctcc tgcgtcatcg
     5761 gcacaatcaa agcgttagta ataatgagct cgctcacttc tctgcacctt tcccgagccg
     5821 tcactagaaa tggctcaatt tagttcccgg ctggatccga agccgtggtg gttgccgcat
     5881 catgccgcta tgcgatactc gaacaatgcc tgacgaccgt tgcttcagcg ctggagttgg
     5941 cgacatcttt cgctccaagc aaggtttacg agtgacgaaa agtatacgcg gccagcacag
     6001 tcgtcaacac ctcggagcag tcaaggtcca agcagactag ctcttgacga aacatctgtc
     6061 cggggaataa gctcggaaac agttcgccgt ggtctcccac ggtgaaaaat aaacaggcga
     6121 ggtgatccat gtcgcaggaa acgaaagtct ttaaatatcg gacgccgcaa ttcgacggag
     6181 tcaaaaaaac cctgcaagtc tgcaacagcg atctaatgaa ggtccaagtg caggtggtaa
     6241 aggatggcgg cgagaacaac ttacactcgc ataccgggga cgatgctttc tggtacgtga
     6301 tcagcggcgc cgtaaagttt tatggcgagg gcgatgaagt tattggtgaa tttaataaag
     6361 gcgaaggcat cttgattcca cgcggattca agtactggtt tgaaagctcg tcgagcgaac
     6421 cgttggaaat tcttcgtgtc actgccaagg atcaaaaggt cgagaatcaa cgagttgacc
     6481 actcagccca aaaacaatgg atggttgatc aaaatacttt cgggacgcgg acgtaaacca
     6541 ggagccactt cgtctctaaa acgcaacatg atccatcgaa cgttttctca gtgatgggac
     6601 atgcttgggg attttggggc cttctgtgtc atttgcgata atttcacggg acagtgtttt
     6661 tgggtacggc gcttgctttt tccgccaagc atggaattca tttgccaaca ttgcgacgaa
     6721 gttgccacag gcgagaagta ccgggtgatc agtgaggaaa acggcctggt atttctaaac
     6781 atggtcgttt gccgctcttg ccagcagcag gccaaacaac tcggtttaca tgccgaacga
     6841 atcacctata gtgatcgttc tcattcgcgc cactccccgc ccgccgatcg attgatggtc
     6901 gggcattcgc attcatagtc ctggctttgc cgcttgcggt ctgagtagcc ggaatatccg
     6961 ttccagttac tcatccgtga gccgggcggc gtgctctcgt gtaagtagct agggccagcg
     7021 tcgtgagtgt ctgcctactt caagtcagaa gcaagccact cagaacaggt cgtgctcgat
     7081 tgagagatac atcagagcac cgtagttgtc tgctttccgg ttgagaccga tcggggcggc
     7141 aaccccggcg attatttgca gcatcggctg atcgacaatt gctgcacgca cgcccggaga
     7201 taagacgggc tcgaacgttc gtcgggtacc gccgttatcg tcgatgcttt cttcaaaatc
     7261 gccgagccat tccaacataa gatgcacacg gcgtgagacc gcgtaaatcg cgctggcgcc
     7321 caggttgtac gataccagtg agtgcttgga tgagagaccg ccgctctcta gaggcgcccg
     7381 agctttgggc aagtagctca atccgaagtt gccgtgcaag gcgaactgcc cgttaatctt
     7441 tttgctgaaa ggcaaacccc attcatagcc gacaactcca tgcccggtcc cccgcctgcg
     7501 gctgccggat ggcagaatca ggctgaatct aggggcgaaa gcaagttcgg attcctcctc
     7561 tttcaagagt tgatagcggt aattgagcaa gacgtcgccg agaccatttt gccgatcccc
     7621 ggtttcaacc aggtgatagg atgggatcgt gtaagaaaat tgatggtttt gcgaaaaaat
     7681 cggccactcc tgagtgaaat taaactccca gccccgtcgc cgcgaatcat tgacgtactg
     7741 cgccgtaaaa atatgttgaa ccacgccggg ttcttgattg tacgcctcct cgatcagaaa
     7801 actattgtcc tcgataacgt caatgggtct ttctttctca gccgcgataa gacctgccgg
     7861 aaatagataa atcgccagtg ccgttacgat ccagtagaag aacttagtgc ccatatggga
     7921 agcttcaata tctttcaagc aaagcctcaa gccggccgcg cgattggtat ccagcgtggc
     7981 tggagttgaa cctgtgttgt cggaaggcaa ttgtcacaac ttcccacaac gaagatggca
     8041 tgttgcgctc tatcccggat agagttcttt aacgaatcct tccttaatca actgctcgag
     8101 cggcgcgaga tcgtaaaatt gtttaggatc ggcgccttta gccttgggat ttcgcaacgc
     8161 aatatcttcc aacaccgatt ccatctctgg ttgggttatg gtcggcacat ttcggatgta
     8221 gccgagcaca taactcttaa acgcgctatt caagagcctc gtgtcggtgt tgcgcgtgta
     8281 tttgcccaaa acttggacgg tgaaatcagg attggctttg gcgtatttga ttccttcaag
     8341 cgttgctttg agaaatcggt tcagcgtctg cggccgactt ttaatgaagg cgccagtggt
     8401 caccagagcc gtcccaggat agcggtactt gatctcgcca aggttcaata attctttgta
     8461 tccgagttct ttggctctct cgagagtcgg tgacgagatc aacccgcctt tgatgacgcc
     8521 ttgctgcatg aacagcaaaa tctccggcac gccgccggtt tgaatcatct tgatgtcagt
     8581 cgccgcatcg aggccaaact cactgacggc ttttctcaag ccaatgtcgg ttaacgagcc
     8641 gaagcggctt actccgaatg ttttgccctt gagatcctgc actcgagcga tatcgggagt
     8701 cgtcacgaga ctcatcggca caacattgat agtcgtgcca atgatggtta catccgcgcc
     8761 agataatcgg gagagaactg cggcagcccc cgagccttgt acgatctgcg tgtcgccgga
     8821 aaccagcgtg gagatcgcct gagcgcccga gacatagacc gtgtttacgt caaggccgta
     8881 cttctgataa atcccggctt ctttcggtat ccaggtggaa accgatgtcg cggctatcga
     8941 tgaataacaa acgttaagct tttcgagttc ggcgccatgc gcaaagggcg ggctccatcg
     9001 taagatcaac agcgcggcga tgaccaaccc gctaccaatt ttaatccgat tcatcggaat
     9061 actctctaat gattcatggg acgcaaccat tcatcagcgg cgaccatagg aaaggcaccg
     9121 gatgatgtca agatattcgg cacaatcgtt cgcgatttct cacatggaga atacaggcgg
     9181 ggattcgccg agcagcgctc ggcaaacgag ggaatgacct acgatggtga tcgccgaaga
     9241 gagaattact gacgacttga tttggaaaag ggtgtcccca gcctggggac acccttgggt
     9301 gctagccaca aactcgagct gaggaggtaa agcccgagct gtgctccggg aacgggatgc
     9361 cgctatcgca ttcgcgccat ggcgaattgc aacttcttga gctcgccctc aagatagagg
     9421 tctttaattt ggtgccggtc gagatgcgcg tctaggagtt ccaccgggaa aatcttggta
     9481 ccacatcgat ggcagactgc agcattatag gtgctgttct cgcagctgat ctgaatatat
     9541 tcggccgtct ctgatgtttc catttgtccc tctctgtcgt gcatttacca tgcgtttcca
     9601 gtgaagaagc aatcaacatg ccgcgattca aaaattagta ggcgcgttct aagacactga
     9661 ttttcttttg aaaatgaacc aagagaaatt agaagacgct gagacccagc gactacagct
     9721 atcaagcaat gtccactcaa cgtctaccat tgacattttt gttctgccgt tcgatagcaa
     9781 gttgatgatc gatccgtcgc cgataccatc cgacaacgtg gtgttctcag aaactcaggc
     9841 aggtcgtttc actctgggtt acatcaaact aaacaatccg cgggcgctaa acgcgctcac
     9901 cctcgcgatg ttccgcgaga tcgaagcgac tttgctgaga tggcgcgcgc gagaagatct
     9961 ggtctgcgtc gttttacact ccgattccga gaaagccttt tgcgccggcg gcgacgttaa
    10021 ggcactggta tcatcgctcc aggcgggatc ggatctcgac tcgggcgctc aatatttcac
    10081 ggcagaatac ttagtagatt atctcatcca catctattcc aagccgctcc tctgctgggc
    10141 tgacggaatc gccatgggcg gcggtatcgg tatcatgaac ggagcaagct accgggcggt
    10201 caccgagcgc acgctgatgg ccatgcctga gatcaccata gggctgtttc cagacgtagg
    10261 cgggacctat ttcctaaatc ggttgccgga aaatttggga ttgttccttg cgctgaccgg
    10321 cgctcgcttt gacgggcgag atgccgtcgc catcggcatg gccgacggcg tcatcgcatc
    10381 ggacaagaaa tcgcaagcgc tggatggatt aggaaatctc gaatggaaat cagatgcggc
    10441 ttatgacaaa gaaattttgc gacgtcatct ctcgcgcttt gccgaatcgc caacgagcag
    10501 cgaatccgcg gtcatgcagc ggcttgagac cattcgatcg ttgaccgaac aatccagtat
    10561 tgaagctgtc gatgccgcgt tacgtggttg gttcggtaaa gacgaatgga tcaaaagcgc
    10621 gatcgccggt tattttagcg gttcaccgac atcggcaaag gcgatctttc gacagatcaa
    10681 cgaaggcaaa aagctcaatt tgaaacaggt ctttctgcgc gaatgggata tggctttgaa
    10741 tttttgcgcg cgctccgatt ttcgcgaagg tgtcagagct cgcctaatcg ataaagatca
    10801 gaagccgcaa tggcgaccgc cggttctcgg ccaagtccaa gaggccgagg tcgagcggct
    10861 ctttgctaaa cagcatggcc agcctgatct tctgtcgcaa agatttgccg aattatagtg
    10921 ggccatatca gtgtgatggg tcacagaaac tatcatttag cttccgcatg caaccgcctt
    10981 gaccggatca atacggctga aatataaccg tcaagagaga attccagcaa aattacgcaa
    11041 aggagtttca cgatgtggct ttcggctctg ttagtcggct tgctcatgct cttctcgaga
    11101 gagaccggtt tctcccaaga tgccaagttg atcgaagccg caaaaaagga aggcggcaag
    11161 gtgatcgtct acacatccat ggaaaccttc accgccgatg cgattaaagc ggctttcgag
    11221 aaaaaaaccg ggctgcaaat ggaatactgg cgcggcggct caaccgaagt tctcgatcgc
    11281 tctctggctg agtttcgcgt cggcaaaccc ctgttcgatg tggtcgcaat gaccggcgat
    11341 cacatgcacc tcttagccaa ggaaggcgtg ttcgtaaaat accaatcacc ttcgttcaaa
    11401 ggcttcgcta aagacgcaat tgacccaaat ctcggagctc gttaccgaaa cgtgctctac
    11461 ggggtgatat acaacaaagc caacatcaaa gctgccgaag cgccaaaaac actggaggat
    11521 gtcgtcaagc cggaataccg cggcaagctg gtcatgcccc atccggtgaa ccacacattg
    11581 accacccaat ggctcgccag tttggacaag attatgccta aacccagggc ggaaaagttc
    11641 atccgtgatt tagcggctgc aaaaccaata tttgtcgaat ccattgtgcc cgccgccgat
    11701 cgggtcggca ccggtgaaac acccattggg attactttcg cgcgcttcgt cttgacctat
    11761 aacaaacagg gcgccaatct cgattatgtc cgcgactaca atatgctcgg cgacggtcag
    11821 tacatcagcc ttggatccaa ggctccgcgg ccgaatgccg gcaaagcttt catcgatttc
    11881 tttctcgacg aagagagcat gacgattcag gcaaagaccg gcgagttcgt caatcgccaa
    11941 ggtatttatc cgcctctggc ggatgccgat aaaattaagt tcgtgcagat gtacccgttc
    12001 agcaaagaag attacgaaat caaaaagaaa gaatatcaga agatttttct acaatgagcg
    12061 ctcgacccat ggcgcgcgaa tagatattct gaaaccagtt tctcagccgg tgttatccgg
    12121 ccgaatagtc ttgcaagagt tttaacgcat caattcagga ggcacccaat ggcggatttg
    12181 gtcatcgatg cagacggaca catcatggag cagcacaagg acctcttcga ccacatcaaa
    12241 ggaaacttcg gcgagatgaa ctggcattcc agttggccac tgttcgacgc cgatggctgg
    12301 cagcgcggct tggcgcgcaa aggcaagcgc gaagatcccg acgccgaggc ttggattcgc
    12361 ttccaaaatg agcatggcat cgatatggca gttctctacc cgacatcggc cctggggctg
    12421 ggtatgatcc aactgccggc atgggcttcg gcgctggccc agggatataa cgattggctt
    12481 tatcaccgct ttaccagcca aacgccgcgt ctcaaaggtg ttgcgctgct tgcgccgcaa
    12541 gatccgaaag cggccgccgc tgagttgcgt cgttgcgtca aagatttagg gttttgcgcc
    12601 gggctgttgc cctcggtcac caacaatcgt actccgctct acggttcgcc gctgtttcac
    12661 gagatctacg atgaggcgga gcgtctcaat gtgccgctta ccattcatgg cggcgtcagc
    12721 caaaacctcg gcctggatcg cgtcgccagc tttttggaag cgcatatgct cgagcatccc
    12781 gtggggctat ttttgcaaat caccaacatg gtcttccaag gggtatttca ggagtttccc
    12841 aaacttcgta ttgcctatct cgaagcgggc gcgggttggg tgccgttcat gatggaccgc
    12901 atggaagaag attacgaaaa atttgccgcg cgcctggcgc ccaatcttaa atcaccgccc
    12961 agcacgtttt ttaaatccgg caatatcttc gtcaccatgg aagtcgaaga gcgcaccgcg
    13021 ccctacattc cggagctggt tcatccggat gtaatcatgt gggcatcgga ctttccgcac
    13081 gagcgcgaac gcgaccaatt cggcggcgac ctccctcacc tgagagcccg taaggacctg
    13141 tccgatgaat tcaagaacaa gatgctgttc gacaacccag tgcgttttta ccgcctgagc
    13201 gaaggagaca tcgcagcagc cagaaaggca aaaggcaatt agccgtcgta aaaaggggac
    13261 cggctcaagg ttcggttcgg gcgatagcgc cgccaccagc gagcgtgttt cagacctgcc
    13321 caacccctca gcggcctaat tccgcgcggc acgcgcagtg ttaccgtcca aacagtttct
    13381 gcacgaagcc ttcgtcttcg agctttttta aaaaccggtc attgatgaat ttccgagggt
    13441 ccgccttaac tgccttcggc tcactatccg cgagcgtttc cacgactgcc tgcatcgcta
    13501 ctaacgatgg ataaggtaca tcggggattc gcgctctatt atactcgtat gcttctttaa
    13561 gcaattcctg atcgtttacc ttgctgaatt gcgaaagcgc tttcaaggtg tattccggct
    13621 cggtcttgaa aatcttgatt gcctctaaat aggcccgtaa gaaactcaac attcgtgctt
    13681 cgtttttctg catcatttga gtactgccac acattcccgt attgaagtag ggaatgttaa
    13741 gcgcaccggc atccgcgaga atgttgaaat ttagtttctt cgcctcggcg ccgaacggcg
    13801 cattaaaaat ggaaccgtcg atgccgccgg acttgagcgc caccagccgc tgggtttcgt
    13861 tgccgatctg gagaatggtc acatcctttg gatcaacgcc aatttttcga agcaagacct
    13921 tggtgctgaa atcagaagag gagccaaacc ggctgatgcc taatcgtttt ccctttagct
    13981 ctgcaggttt cttgacttcc tggcggctga ccagttgata acccattttg tcatcggtca
    14041 tggcgatgta gacaacatcc gcgcctttca tattggcgat cacgccggga gggccagtta
    14101 gttgagccaa gtcgatgtct ccggacacca gcgcttgaat tgcgaccgag ccgccgggaa
    14161 cgtaaacaag cgtggatgag aggccgtgtt tggcgtacag ccctcgtttc tctgcgatcc
    14221 aaaccagtaa ggcatgtggg ttcacggagc tgtaggcgat ccgaatcggc tcggcagcgc
    14281 gggcgatgcc gcgcacaacc gatgacatag caaacaggga taaaaatact accgcaacaa
    14341 gtcgtctcat gagcacctcg gcgatctcta tagagcgaac agctccttgg cgttgtcgtg
    14401 aagaatcttt cgcttgacct cctcggacaa gtcccgatga ttgcgtaaat cgatcaaatt
    14461 ttccgggaat tcgttgtccc aatgcggaat gtccgaagcg taaagaaaat gctcggcgcc
    14521 gacatagttg accgtggcag cgagctgcga ttcgccgggc tcgacactga aatacatctt
    14581 cgattgccgg acaatatcgc tgggcttgtt cttgagctgc ggcatttcgt acttgccgcg
    14641 cttctcccaa tgttcgtcca gccgatccag gtaatagggc aaccaagtcg cgccgatctc
    14701 cagaaaggcg agcttcagtt tggggaaacg gatcggcagg ccctggcaaa ccatgctggt
    14761 aaattgcagg agtatcccaa ccggaaaggc gtaggagtga acctccgaga aagttgccag
    14821 tccggacgtg ccgagctcgc gtgatgtatt gcgggtgcca tgaatgccga ggaccgcgcc
    14881 gagcttttca gcctgggcat agatcggatc gtagaaacta tctccgagcg ccagcggcaa
    14941 acccgtcggc aagacctcaa atccttttag gcccagctct tccactgcac gggtcaactc
    15001 tgcggcggct gctgcgggca agcgcatcgg caaacagccg acgcaagaca gacgatccga
    15061 gcggtcgttg tactctttcg cgaaatgtgt gttgctcgct cgtgcgacgg cgatttggta
    15121 cggcacttcc tgatttttcg cgatattccc ggaccccgtg ggaaagcaga tcgccttctc
    15181 gatatcgtgc tcgtccatga tcgcgtgcca acgctccact tgctcggcgg cgctgagtgc
    15241 tttccactgg ctggccatct cgaatgagtc catcatgtca ttatcccagg gctggtcacc
    15301 gggccataac ggggtcggac gtttattcca cggcggctca agatactttc tgatatcgga
    15361 ctgccgttct aagacatggc catcggtatc gattaccgga aagtgcgcca taaacccctc
    15421 ctgagtaaac ctatgggtaa gaaaccttta gtccggttca aagggatgtt caatggctga
    15481 gcacggacaa tttttatgta atcctcggtg tcgaggcgat gcgacaaagc gcgttcccgc
    15541 gtccgtgttg tccggatgat tgtgcgacat ttctctgagt gtgctgtcac aggtggttga
    15601 tcaaagactc gcgaaagtga ccctcgttag aacgctgttc atacgaaatt gccgcactca
    15661 gcagcgtttt ttcacggtac aaggcttggt tctcacgccg ctcatgtcaa tctagaaacc
    15721 actctccagc accacaccca tccttgacga gattgcccgc atgacatacg acaagggaga
    15781 aacgctcagt tctcattagg agggaatcac atgggtttac acgtcaaaag ctcggacgta
    15841 accgaaataa agcgcgtcca aagcggcgag gcgggtaccg gatcgatggt tgttcgcaaa
    15901 gggtatggga acgagtgcag cctgatgatc gcgactcgtg cgcccggcta tcacaccaaa
    15961 ccgcatcagc atgagtccga gcagattaac tatatcctcg agggtgagat ctggttcttt
    16021 gttgaagata aaggtttcca ctgtaaaaaa ggcgacttcc agcgtatccc ggccaatacc
    16081 gttcattggg cttggaatcg gtccgagaac gaggcgatgg tagcagaagc gcacgccccg
    16141 gcattgatcg gtggccgtgc cggtgagtac gccgtgggat tatttgacga cggagagaag
    16201 ccccaggttc gcggtccggg cgtcaaccag ttcgtgcctt tcgaccagca gaaagccgaa
    16261 gctaaatacg ctagcaaata attaaagcgc catgctttgg aaagtttcat tgatcgtgag
    16321 ctttctttgt ggtctgctgt cgatccagaa actaagctgg gctcaggacc tccgccgcat
    16381 ccactacggc accactaact ccacctctca cctgcccgtc tgggttgcta aggatgcggg
    16441 ttacttcgcc aaaaacggcc tgaacgtcga gcccgtccac atacgtggcg gcgcattgat
    16501 caccatgggc atcatgagcg gccagctaca attttccggg gccggcgccg agtccgtagt
    16561 cgcagcgcgc atcgagggcg gcgatgtcgt gttgctggct tgcccggtat actccgatcc
    16621 ggtttatttg atggcccggc ctgagataaa gtcagccgcc gaactcaaag gcaaatccac
    16681 tgcggtcact cgtctcggct cgacaacgca tttctatctc cgggccgcgc tccgccatgt
    16741 cggtctcgat tcggaaaagg atctaaccat actgcaactc ggtgccggac cggagatcgt
    16801 cagcgccttg gagacaggtc gcatcgccgc cgctgctctg accacccgtt acgcgctccc
    16861 ctacctggaa cgcggttggc cgatgctcgt cgatctcagc accaccgact tggtatatcc
    16921 atcgtcttgc gttgcgagca gccggaactt cgtcaagact gaaccccaag tggtcgagaa
    16981 ctttctcaag gcctacgttg acgcaataca gctcatcgtc aaagatccgc cttccgcaga
    17041 gaagtcattc attagatggt tacgcgaacg agatccgatg gtgattaaaa aatctgtgaa
    17101 cgcttacgtc cggctgttta agaccaagcc ctacgtaccc gacaagggaa tagaaaatgt
    17161 gctcacagat cttgcaactc ggcgagccat accgaaggag tttattggcc gtccggaact
    17221 tttccgagac aacgcaccac tagaaaaagc cttatcgtcg cgaccatgag gtatcaatcc
    17281 ttcatagtct tgctgacctt ggtgtgcgct ttcttccgaa cctaccaggc cgtagacgct
    17341 caggaactta gacggattct ttatggcacc agtgcatcgc catcgcacct gccggtttgg
    17401 gtagcgaagg acgctggtta cttcgaaaag tacggtttca atgtcgagcc cgtgcaaatt
    17461 cgcggtggat cgctgatcac tctcgcaatc atcactggca gcttgcagtt ctccggtgcc
    17521 ggcgctgaat cggtgattgc cgctcgcgcg gcgggcgcag acgtgatcct gctagcctgc
    17581 ccggtcgatg tagatccggt gtatttgatt acccgtccgg aaataaaaac ggcggcggag
    17641 ctcaaaggac atgccagcgc cgtgacccgc tacggctcga ccactcattt ttatctcaga
    17701 acagcgctcc ggcatgtcgg tcttaatccg gaccgggaca tgaccattct acagctcggc
    17761 gccggccctg agatggttgc ggccctggaa acggggcgca tcgccgccgc ggctctaacc
    17821 gctcggtatg caattccatt tctgcagcgc aattggccgg tactggttga tctgagcgca
    17881 acggatctca tctacccgtc atcttgcgtc gccagcagcc gggccttcat gagagccgag
    17941 cccaagacca ccgaggactt tctccgagct tacgtcgcgg gaatccaatt gatcaagcgc
    18001 gatcacgctt ttgcggaaaa atctttatcg aaatggctga gagaaaacga ccccgtgata
    18061 ctcaagaaat cgatccaagc ctacgcaaaa ctgttcaagc gagctccatt cgtatcggat
    18121 aaagggattg ataacgtcgt caaagacctg gtcagcatgc ggccggagtt taaggaatat
    18181 atcggctggc ctgaaccgtt ccgtgagaac ggtccgctgc aaagtgcgct gggccgccgc
    18241 tgagctaacg catctccaaa aactccagcc gaacgccttc cggtccctcg aaaaaacaaa
    18301 cgtgacgacc gtcgggtagc acgcgctctt cgaggatttt aatccccgcc gccttgatct
    18361 tggcgacttc cgcggcaaag ttgtccgcat cgatggcgac atgctccaaa ccataatgct
    18421 gctccagttt ttgatccgac aaatagtcgg tgatgttgag cggcacgccg tgcaaatcca
    18481 gacgaaaagt tttccgtccg tccggacctg cgcgctctga gacgatcttg gcgccggcga
    18541 actcgacgta aaaatctgcc gtcttacgcg ggtccggcgt tttgagatgc aaatgattta
    18601 ctctgaaacc catgactacc tccgtgatcg gccgaaacgt tgaccgcttt ctactcttgc
    18661 cgacttcagt attgcaagcc agagaaatat tcaagcctca ggccacatga cgtgatcatc
    18721 agaaagttcg ctgcgctgaa gctatacgag gacgcgggtt cttccaggac gaccctgact
    18781 catgtaaggt cggcaaattg actcccctgc tatgtcggga aggattcgat tacgcagtcg
    18841 ttaagctaaa ctcga
//
LOCUS       DASUVS010000042        11284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_233134, whole genome shotgun sequence.
ACCESSION   DASUVS010000042 DASUVS010000000
VERSION     DASUVS010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11284
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233134"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VF182_02560"
     CDS             complement(<1..276)
                     /locus_tag="VF182_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435985.1"
                     /translation="MKQTFVSAVAILLTLALFTPIDLHAEKIRVGSGGFTPLHSIIWA
                     AHDQGVFKRYGFDVEYLALNSGTLGAQTLLSNEVQFLFSTGALAVTAN"
     gene            745..1128
                     /locus_tag="VF182_02565"
     CDS             745..1128
                     /locus_tag="VF182_02565"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011849779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6435986.1"
                     /translation="MPKKEIVQVKLAAANPNLSPATRFGNLVFVAGQTGRHPITGEVG
                     RDVREQTRYALERIKMILETAGTSLDNVLTVLTHLTKREDLAAYNEEYANYFPTNKPA
                     RTTVEAMLNFPELLVEITVTACIPE"
     gene            complement(1753..2892)
                     /locus_tag="VF182_02570"
     CDS             complement(1753..2892)
                     /locus_tag="VF182_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6435987.1"
                     /translation="MQRLEERLRTSSYAAAVDFSFVLEYWASYPMKKENKRSGFSFGR
                     PKRPAINAPFSLIVVAVIFIAAIGAAARAADQVTTAYQSVSIGSSTPLWIAKDAGFFE
                     RQGLDVKIVFVEGSPRTMQTLIAGESQIVESTGPAVLNARAAGSPVVIVAGYVNVMPY
                     YLIVDKSINSPGDLKGKVGANHIPGTAADTVMRIGLKALGLDPDRDVSLRAVGNTPMR
                     LQAMSAGFAQFMVAQDLELQQAKRLGYKVLVDYVANKTPFPMGGAVTTQRYVKEQRDT
                     VLRYVKALAQALHYLKTNREGSLTIISRYARFQNAEVMGAAFDSAQRLYSEIPLPPTE
                     GFDLVARELAQRNPKLRDFDTSSVVDLQFVRELEQSGFLKSLKQN"
     gene            3076..3249
                     /locus_tag="VF182_02575"
     CDS             3076..3249
                     /locus_tag="VF182_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435988.1"
                     /translation="MSAPAPTIWKEYRLLLYSAVIGIVGGLGAQLFVWLLNSAQRLFL
                     VGFAAINHLSQEL"
     gene            3297..3770
                     /locus_tag="VF182_02580"
     CDS             3297..3770
                     /locus_tag="VF182_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEX6435989.1"
                     /translation="MIAFIVERAVSAGFKYPLLYEAQVELRSDLPTHLESMLKATFAV
                     LERGPLVDLRNVTFPHLASLLRHGTPIPIHGGQGSLVTANIADNSQIAGRTIAEVFER
                     FPQLLAVAIIRDQRIELPRGSTALESGDQLLVVGSETKSIETFTRVATNSLYACG"
     gene            complement(3861..4973)
                     /locus_tag="VF182_02585"
     CDS             complement(3861..4973)
                     /locus_tag="VF182_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6435990.1"
                     /translation="MSRGVLVVTALILAALVGAAAAQTSQGQAPEWDKILAAAKKEDK
                     VVVSIPPSRKLRRSVEVAFPRRYGIGVEFVSVRGSASIQKMISEAKTRIPYVDLHVGS
                     AESAITRLLAEKALEPLEPYFVLPEVKDPKQWWGGHMWMDNAKSFIYAFAAYQTVSLW
                     CNPNEYTPAEFQSFDDLLNPKLQGKIGISDPRTPGSGNSMWSHMLSVKGEEYLKKLVA
                     QKLFVTRDPRLLGENLSSGKIAVTLGIGYSELLPFIKAGVSVVPLSYPKEGFYATSGQ
                     GNLMVIKNPPHPNAAKVFANWLLGRDGQEIFGRSMSVASRRLEVDTQWLKEFGVIGSK
                     DSLTVEQFYRLENQSEEKFYKHRELGAAAARRLLGS"
     gene            5403..6404
                     /locus_tag="VF182_02590"
     CDS             5403..6404
                     /locus_tag="VF182_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6435991.1"
                     /translation="MKAEISVFSFHNSCSVSPWAVFNRLLPLLLIASIAHTVGCTRQF
                     PRTQVTTIKAANPAELQRYLLSRKPDVAQFRFRGPFAMVERRDLEIPLDSDLTVTADL
                     YLCAAANKVPLVIVLHGNNNSKEDHAFQAMHLASWGMHGLAVDLPKRGPWIGNGRILA
                     KLVDTIHRTPQLIDGRIDGDRIILVGHSFGATAVATALGEGAPALGGVLLDPTAIGRG
                     LSQLLTRITVPVMVIGADEDIRPARNRGPFYRFIPRAVGEISIRDTVHEDAQYPNQNT
                     LRTFEDDPDDTEEAQIAFVSALTASAFSLAATGDIDYAWNSFENALKNGFFFNGRRK"
     gene            6410..7318
                     /locus_tag="VF182_02595"
     CDS             6410..7318
                     /locus_tag="VF182_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435992.1"
                     /translation="MRTVSSSVLGWQIAGARLAVLMIIVVQALELRAGWSQESVPRIN
                     EEFLEQERIYRSRGAEGRRVYTTDRGLSRYADLLPSTFCDGLARLGSSDRWLDIGAGQ
                     AQAILDYYLPENAAAPAENCRGSGSKARAVAISIEDRRTENWKQQVAGLSDDRIRYLY
                     GKRLRQYSPEELGKFQIITDVYGGFTYTEDLSQFVGKVLGLLEVGGSFYTLATGVHLE
                     DGKDKLGTLYLTELEDAFGRALKMCSWLKKISCVQVTCESKTDLKRPTQLIKIEKICS
                     DTSVPSMKLVEFEAGYPPARCFQLDP"
     gene            7396..8262
                     /locus_tag="VF182_02600"
     CDS             7396..8262
                     /locus_tag="VF182_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009160800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6435993.1"
                     /translation="MKTEISAVIFHISSFLMSSLLLAILYLLGTASFAAANTFDDALA
                     VYERGNYVQAMKLFRELAEKGHQWAQRRVGLMYAEGKGVPQDYHEAVKWYGLAAAQGN
                     VPAQYSLGLAYENGQGVSQDYQEAVKWYRIAAAREDEWAQTRLGRIYAEGKGVPRDYQ
                     EAVKWYRLAAAQGYAPAEHSMGVAYEKGQGVPQDYREAVKWYRLAAARGNELAQVDLG
                     MMYTKGRGVAQDLVRAHMWFDLAAAASTGNSRDTATENRDRIASKMTTEQIGTAQEMA
                     RRCRESRLKNCD"
     gene            complement(8642..10435)
                     /locus_tag="VF182_02605"
     CDS             complement(8642..10435)
                     /locus_tag="VF182_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX6435994.1"
                     /translation="MNDTTETTQDRLADFTRDRRVIVLALMALIIGLLSALTATGLVW
                     LIAVITNLAFYGQFSSVLQSPVDHHLGYLVIAVPIIGGLMIGLMARYGSEKIRGHGIP
                     EALEAILFGRSRMEPKIAVLKPVSSAISIGTGGPFGAEGPIIMTGGAIGSLFAQFFRL
                     SSVERRVLLVAGAAGGMSAIFASPVAAALLAIELLLFEWRPRSFIPVAIAAAAAAALR
                     VPLLGPGPIFPVHAHLALNGEGLLFAFFVGTVAGLGSGLLTNLVYACEDLFGKLPIHW
                     MWWPAIGGLCVGIGGLIEPNVLGVGYETIHDLLKGNIVGTALLGILIGKSLVWSIALG
                     SGTSGGVLAPLLMMGAALGGGMANFIPLGDSGLWALIGMAAMMGGTMRSPLTAMIFTV
                     ELTQDWNLLPGLLVGCIAAHGVTVLLLRRSILTEKIARRGYHVMREYGVDPLTMVRVG
                     EVMDRNPPIIASNTPLVELSDRIALRDPEVSRHEGLLIVDKRRHLVGIVTRGDVVRAL
                     EQNGNGDTTVLSAGSQDLVVTFPDELLFEAASKMLHNDVGRLPVVSRRDPRHIVGYLG
                     RSNLMAGHLRQLEEEHVRERRFALAKRTADV"
BASE COUNT         2576 a         3143 c         3196 g         2369 t
ORIGIN      
        1 attggcggta acggccaaag caccggtaga gaaaagaaac tggacttcat tggagagtag
       61 agtttgtgcc cccaacgtcc cgctgtttaa agctaggtat tcgacgtcga agccgtatct
      121 tttaaacacg ccttgatcgt gagccgccca gataatcgag tgaaggggtg taaagccgcc
      181 gctgccgaca cggatttttt ctgcatgtaa atcaataggc gtgaataacg ccagggtcag
      241 tagaatggcg accgccgaga caaatgtttg tttcacggga actcctacag aagacaagcc
      301 tgtccctctg ctgtccggac ttcatacgaa gcatttatat ccggtcgaac caagagaagc
      361 tccgggatct ggcaagagaa cgtagcatga aagacatcag cccttcaaag ttcaacggtc
      421 aaatcttcag gatcaaacgt tcggttgtcc acatctccga ctgtttcaat ggtgcacaca
      481 ctaacgacga gcgaagttga aatgttgcat tctgttcaag accagtgttc gtttacgatt
      541 gtatcaaaat gttcaaggtt ggcgggcgaa gccgccaaga gcgtttcagc aagaaacgct
      601 atcgcgtttg gctcggatta cggattcgaa tggagatgct agcatgaacg cgatccaaaa
      661 tggtccgctt cgttacgttc aaatatagaa aaggatcggt gcaaatagat ccttcattaa
      721 ttagctttcg ctaaaggaga ccagatgcct aagaaagaaa ttgttcaggt gaagcttgcc
      781 gcggcaaatc cgaatctctc accagcgaca cgtttcggta atctggtgtt cgtggccggc
      841 caaaccgggc ggcatccgat taccggcgag gtaggcagag acgttcgcga acagacccgg
      901 tacgcccttg aacgcatcaa gatgattttg gaaaccgcgg ggacctcgct cgataatgtt
      961 ctgaccgtgt tgacgcatct aaccaaacgt gaggatcttg cagcctacaa cgaggagtac
     1021 gccaattatt tcccgaccaa caaaccagcc agaactactg tcgaagcaat gttgaacttc
     1081 cccgagcttt tggttgagat aaccgtcaca gcgtgcattc ccgaataggg cagtcctatc
     1141 ttagaagatg tccgatcgtt aactcacact gctgagccat gttgccccac ggttgaaata
     1201 ctacagatat tattgccgtc cttcggtaaa cgagtggcat ttagacacca agagatatgg
     1261 caggacttta ctgaaaaaag gtttttgcac cggtagcgga gtggcggctg aaatcggaac
     1321 cgtggacttt cgtggtcaat cccgatggga ttgtcgatgc aaagttcgag tggattggtc
     1381 actaacgacg aattgaaaac cgcactgcaa aaagttccaa actaaatcta tgcgcagaag
     1441 cgggcatgtt aacatacaac agaggaggaa aaagaatgat aagagcgatt gttccggcgc
     1501 tagaactccg cttgacgcac tctcgcaaaa tggaagttcc cggactccat tttgtccgaa
     1561 tgcgctcaat cgagaagtga ggggtcagaa gaattctcag aacgatctta gcgcaacgaa
     1621 cgcgaccgcg aagaaattgg atgcgctgtt ccgacacatg tgccaggcag ttcgctcgtc
     1681 aaagcagagc aattataagg gcaaagcttt gatccggagt ttcactgcga aaaccatcct
     1741 cggctcgccg gactagttct gctttaggct cttcaaaaag ccgctttgct cgagttctct
     1801 tacaaactgc aggtccacga cagagcttgt gtcgaaatcg cgtagctttg gattgcgctg
     1861 ggcaagttcc ctggcaacga gatcgaaacc ttccgttggt ggcaaaggaa tctcgctgta
     1921 cagacgctgg gcagaatcga aagcggctcc catcacctca gcgttctgga aacgcgcgta
     1981 gcgggagata atcgtcagcg agccttctct gttcgtcttc aaatagtgca acgcctgagc
     2041 cagggctttt acgtagcgca gcaccgtgtc acgctgctct ttcacgtagc gctgagtcgt
     2101 cacggcgccg cccatcggaa agggggtttt gttggccacg taatccacga gaactttgta
     2161 accgaggcgc ttcgcctgtt ggagttccag atcctgagcg accataaact gagcgaagcc
     2221 cgccgacata gcctgcaaac gcatcggcgt gttgccgacg gcgcggagtg acacatcccg
     2281 atcgggatcc agcccaagcg ctttaagacc gatgcgcata acggtgtcag ccgcggttcc
     2341 gggtatatga ttagcgccga ctttgccttt caggtccccc ggagaattga tcgacttgtc
     2401 gacgataaga tagtacggca tcacattgac gtaaccggcg acgatgacca cgggcgagcc
     2461 ggccgcgcgg gcgttgagca ccgccgggcc ggtggattcg acaatctgcg attctccggc
     2521 gatgagcgtc tgcatagtgc gggggcttcc ctcgacaaag acaattttca catcgagccc
     2581 ttgccgctca aagaaccccg catccttggc gatccacaat ggggtggagc ttccgatcga
     2641 cacggattga tacgccgtcg tcacctgatc ggctgcacgt gcagccgcac cgatcgcggc
     2701 gataaaaatg accgccacaa cgatcaagct aaaaggggcg tttatggccg gccgcttggg
     2761 ccgtccgaaa gaaaaacctg atctcttgtt ttcttttttc attggatatg aggcccaata
     2821 ctccaagacg aaagaaaagt ctactgctgc tgcatagctc gatgtcctga gccgttcctc
     2881 caagcgttgc acgccgctcc ccctctcaga taagttattc cagacgcgtc gaaagatcga
     2941 ctctgttgta tcgagaccca gcggccggtc cagcatttta cccgaagctc acctagatcc
     3001 cgttcgtcga tgctggagct tcccggtcat cccgccaaat gagggcaagc attcaggttc
     3061 cttgctgatt tacgcatgag cgcacccgcg cccactatct ggaaagaata ccggctgcta
     3121 ctgtactcgg ccgtgatcgg catcgtcggt ggattgggcg cccagctttt cgtttggctt
     3181 ctgaactcgg cacaacgtct tttcttggtt ggatttgccg ctatcaacca cctgagccag
     3241 gaactttgaa tcccgaggcc gtcataggac cgtggggttt gtggctggtg tcgaccatga
     3301 tcgctttcat tgtggagcga gccgtttccg ccgggttcaa gtatccgctc ttgtacgagg
     3361 ctcaggtcga gctaagatcc gatttgccga ctcacttgga aagtatgctc aaggcgacgt
     3421 ttgcagtgct cgagcgcggt cctctcgttg acctgcggaa cgttaccttc ccgcatttgg
     3481 catccttgct ccgccacggt acgccgatac ccattcacgg tggccaaggc agtctcgtga
     3541 ccgcgaatat cgccgacaac tcgcagatcg ccggccgcac aatagcggaa gtgttcgagc
     3601 gctttcccca gctgctcgcc gtcgcgatta tccgcgatca gcgcattgag ctgccgcgcg
     3661 gttccaccgc tctcgaaagt ggagatcaat tgctcgtagt cggcagcgaa acgaaaagca
     3721 tcgagacctt tacgcgtgtg gctacgaaca gcctgtatgc ctgcgggtag tcgcacagcg
     3781 cgccacgaag gggccgcaac gtgagtcatg cgtagaggag ggtgtgaatc cgaaacagcc
     3841 acggctgtat cggccctcca tcaggagccc aatagcctgc gcgccgccgc ggcgcccagc
     3901 tccctgtgtt tgtagaattt ttcctcggac tggttttcca ggcggtaaaa ctgctcgacg
     3961 gtgagcgagt ccttcgatcc gatcaccccg aactctttga gccattgcgt gtcgacctcg
     4021 agacgccggc tggcgacgct catgctccgg ccgaaaattt cttggccgtc ccgacctaat
     4081 agccaattgg cgaacacctt ggctgcgttg ggatgaggcg ggtttttgat gaccatcaga
     4141 ttgccttgac cgctggtggc gtagaatcct tccttcggat aggacagggg cacgaccgac
     4201 acgccggctt tgataaacgg cagcagctcg gaataaccga tccccaaggt gacggcgatt
     4261 ttcccgctgc ttagattctc gcctagtaag cggggatccc gcgtgacaaa aagcttctgc
     4321 gcaaccaact ttttcaggta ctcttcgcct ttgaccgaaa gcatgtgcga ccacatcgaa
     4381 ttgcccgatc ccggcgtgcg cggatcgctg atgccgatct tgccctgcaa cttggggttc
     4441 aagagatcgt cgaacgattg aaactccgcc ggcgtgtact cgttcggatt gcaccacagg
     4501 ctcacggtct gataggcggc aaatgcataa atgaagcttt tggcgttgtc catccacatg
     4561 tggccgcccc accactgttt gggatctttg acttccggta ggacgaaata aggctccaac
     4621 ggttccaatg ccttttcggc cagcaacctg gtaatggcgg actcggcgct accgacatgg
     4681 agatcgacgt acgggattcg agtcttggct tcgctgatca ttttttgaat gctcgcgctg
     4741 ccccgcaccg acacgaactc aacgccgatg ccataacggc gcggaaacgc aacctcaacg
     4801 ctcctacgga gcttacgact cgggggaatc gagacaacca ccttgtcttc cttcttggcc
     4861 gctgcgagaa tcttgtccca ctcgggggcc tggccctggc tggtttgagc cgctgcggcg
     4921 ccaacgaggg ctgccaaaat cagggccgta accaccaaga ctccgcgact catgcgggct
     4981 ctcctctgaa ctcgagcaat catcaaaaaa gtttctccgt taacgtcatg gccggcttgc
     5041 gtttgcccgc cgatacaagc ctaaggagtc gaaagcggcc cggaaacacc ggccctgtgc
     5101 cgcgcgccct attgcgggca gatatggtcc gcgatggact tctcattatg ttatgtcatc
     5161 ttttcgatca agggctgaaa tacgaaaact ggattggcca atggtggtgg ttgccggcag
     5221 tacgtccgag aatgatttag gaccgagtct cgactgttga ccaactcacc caaacgcgct
     5281 gttccttacc cgatacgaat gagcttgata ttcatgcgtg ccgagtgttg aaaaactttg
     5341 accaaggtgg ttcaaaacgg tccggatgca agagacggaa gatgatggat gaaagcggaa
     5401 ggatgaaagc agaaatttcc gttttcagct ttcataattc atgttccgta agtccgtggg
     5461 ccgttttcaa ccgcctgttg cccctgctgc tgattgcctc gatcgcgcat acggttggtt
     5521 gtactcgcca gtttccgagg acccaagtta caaccataaa ggcagcaaat cccgccgagc
     5581 tccagcgcta tctattgtcc cgcaagcctg acgtagcgca attcaggttt cgcgggccct
     5641 tcgccatggt cgagcgcagg gacctggaaa ttcccctaga ttccgatttg accgtgacag
     5701 cggacttata cctgtgcgcc gcggccaaca aggtgcctct ggtcatcgtg ttgcacggca
     5761 acaacaattc caaggaggac catgccttcc aagcgatgca cttggcctcc tggggaatgc
     5821 atggcctcgc cgtcgacttg cccaaacgcg gcccgtggat cggaaacggc aggattcttg
     5881 ccaagctcgt cgacaccatt catcgaacgc cgcagctcat cgacggccga atagacgggg
     5941 acaggatcat tctcgtcggg cactcattcg gcgccaccgc cgtggccacc gctttagggg
     6001 aaggcgcgcc ggcgctgggc ggtgtcttgc tcgatcccac cgcgatcggt cgggggttgt
     6061 cgcagttgct aacgcggatc accgttccag tgatggtgat cggcgcggac gaggacatcc
     6121 ggcccgcccg caatcgcggg ccattttacc gcttcattcc acgtgccgtc ggagagatct
     6181 cgatacggga tacggtgcac gaagatgcac agtatccgaa ccagaacacg ctgcgcacgt
     6241 tcgaggatga ccccgatgac acggaggagg cgcagattgc tttcgtcagc gcgctcactg
     6301 cctccgcctt tagccttgcg gccaccggcg acatagacta tgcctggaac agctttgaaa
     6361 acgctttaaa gaatggcttt ttttttaacg ggaggaggaa gtagtcgtca tgcggacagt
     6421 tagttcgtcg gtgctaggat ggcagatcgc gggagcccgc ctggcggttc tgatgattat
     6481 cgtggtgcag gcgcttgagc tgcgtgctgg ctggtcgcag gaatccgttc cacggatcaa
     6541 cgaggaattc ctcgagcaag aaagaatcta ccgcagccgg ggcgccgagg gtcgcagggt
     6601 ctacaccacc gaccgggggc tctcgcgcta tgcggacctt cttccctcca ccttctgcga
     6661 cggtctcgcc aggctgggca gctcagaccg atggctcgat atcggagcgg gtcaagcgca
     6721 ggccatccta gactactacc tacccgaaaa tgccgcggcg ccggcggaaa actgccgcgg
     6781 gtccggctcc aaggcgcgtg cggtcgcgat ctccatcgaa gaccgccgga ccgagaactg
     6841 gaaacagcag gtcgccggcc tcagcgatga ccgtatacgt tatctttacg gcaagcgcct
     6901 gcggcaatat tcgcccgagg agcttggaaa attccagatc attaccgacg tgtacggcgg
     6961 tttcacctat accgaggact tatcccagtt cgtaggcaag gtactggggt tgctggaggt
     7021 cggtggtagc ttctacacgc tggcgaccgg cgtccactta gaggatggca aggataaact
     7081 cggcaccttg tatctgaccg agctggagga tgccttcggc cgcgccctga aaatgtgctc
     7141 ctggttgaag aagatctcct gcgtgcaagt cacctgcgag tcgaagaccg acctgaaaag
     7201 acctacccag ctaataaaaa tcgaaaagat ttgcagcgac acttcggtcc cgagcatgaa
     7261 attagtggag ttcgaggcgg gctatcctcc cgcaaggtgt ttccaattgg acccatagtc
     7321 cgcgtgcaac tgattccagg ctgggcgaaa aagttcaaaa tgcgaggcac gaaagacaag
     7381 gatgaaagcg gaaggatgaa aacggaaata tctgctgtca tttttcatat ttcatccttt
     7441 ctgatgagca gcctgctgct cgcgatcctt tatttgctgg gcaccgctag ttttgcagca
     7501 gcaaacactt ttgatgacgc tctcgctgtg tacgagcgtg gcaactatgt tcaggcgatg
     7561 aaactctttc gcgagcttgc cgagaaggga caccaatggg cgcagcggag agtaggtttg
     7621 atgtatgcag aggggaaagg cgtgccgcag gattatcacg aggctgtaaa gtggtatggc
     7681 ctcgcggcgg cgcagggaaa tgtaccggcg cagtatagtc tcggtctggc atatgaaaac
     7741 gggcaaggcg tgtcgcagga ttatcaggaa gccgtgaagt ggtatcgcat cgccgcagcg
     7801 cgggaagatg aatgggccca gacgaggctg ggtaggatct acgcagaggg gaaaggcgtg
     7861 ccgcgggatt atcaggaagc ggtaaagtgg tatcgcctcg cggcagcgca gggatatgca
     7921 ccggcagagc acagtatggg tgtggcgtat gagaagggcc aaggcgtgcc gcaggattat
     7981 cgggaagccg tcaagtggta tcgtctcgcg gcagcgcgag gaaacgaatt ggcgcaggtc
     8041 gatctcggta tgatgtatac caaggggaga ggcgtggcgc aggacctcgt gcgtgcgcat
     8101 atgtggtttg atctcgctgc cgcagcatcg actggcaatt ctcgagatac ggcgacagag
     8161 aatcgagata gaattgcgtc aaaaatgaca accgagcaga ttggaaccgc gcaggagatg
     8221 gcacgacgct gtcgggagtc taggcttaag aactgcgatt gaacaatggg tattgcgtta
     8281 ctagatgata ccgcccttcg tccacgcagc ccatgctttc gcggaaatca cgtactaagc
     8341 tcctgaatat ataatcggag ctcggtcaat aggtgaccgg ttacctggct gaaactagct
     8401 ctgcacggtc agatgcggtg acatgcccgt tataacctat cgcgaccttc tcccagtact
     8461 ggaagctgcg agagccatag ctcagttctc gtatctcaga attttcgccg ggtcatgggt
     8521 agaagaaaat taatttggta tgcgcgcttc accattatcc aacggccgga ataatgtttg
     8581 atgaccccga acgtttcatg agcgacccac aaattaccgg ccgactgcta tagatctcgc
     8641 ttcaaacgtc agccgtccgc tttgccaacg cgaagcgcct ctcgcggacg tgctcttcct
     8701 ccagctggcg cagatgccct gccatgaggt tgctacggcc gagatagccg acgatgtgac
     8761 ggggatcgcg ccgactcacg accggtagtc tcccgacatc gttgtgcagc atcttgctcg
     8821 ccgcctcgaa gagaagctcg tcggggaagg tcaccacaag gtcctggctg ccggctgaaa
     8881 gtacggtggt atcaccattg ccgttttgct ccaatgcgcg caccacgtcg ccacgcgtga
     8941 cgatgccgac gagatgtcgc cgcttgtcga caatcagcaa tccttcatgc cggcttacct
     9001 caggatcacg gagagcgatg cgatcggaaa gctcgactag aggcgtgttc gaagcgatga
     9061 tcggcggatt tctgtccatc acctcaccga cgcgaaccat tgtgagcggg tccacgccgt
     9121 actcgcgcat gacgtggtaa ccccgccggg cgattttctc ggtgagaatg gagcggcgca
     9181 gcaacaaaac cgtgaccccg tgcgccgcaa tgcagccgac gagcaggccc ggaagaagat
     9241 tccagtcctg ggtcagctcc acagtaaaaa tcatcgcagt cagaggcgaa cgcatggtcc
     9301 cgcccatcat ggcggccatg ccgatcaaag cccaaaggcc gctatcaccc agcggaatga
     9361 agttcgccat accaccgccg agggccgcgc ccatcatcag cagcggcgcg agcacgcccc
     9421 ccgaagttcc cgagccgagg gcgatagacc agacgagcga cttgccgatc aaaatgccga
     9481 gcagcgcggt gccgacaatg tttcctttca ggagatcgtg gattgtttcg tagccgacgc
     9541 cgaggacgtt tggctcgatg agaccgccga tgccgacgca caggccgccg atcgcaggcc
     9601 accacatcca gtgaatcgga agtttcccga acagatcctc gcaggcgtag acaagattcg
     9661 tcaggagccc cgagcccagg ccggcgacgg tgccgacaaa aaatgcgaac aggagtcctt
     9721 cgccattcag agcgagatgc gcatgaaccg gaaaaatcgg tccaggaccg agcaggggaa
     9781 cacgcagagc cgcagcggcc gcagccgcga tggccaccgg aatgaacgag cgcggtcgcc
     9841 attcgaaaag cagcaactcg atagccagca atgcggcggc gacgggtgat gcaaagatcg
     9901 ctgacattcc gcctgcggcg ccggcaacca acagcactct gcgctcgact gaagaaaggc
     9961 ggaagaactg cgcgaacagc gatccgatgg cgccacccgt catgatgatc ggcccttcgg
    10021 caccgaacgg tccaccggtg ccgatcgaga tcgccgagga gaccggcttc agaaccgcga
    10081 tcttcggctc catgcgactt cggccgaaga gaatcgcttc gagtgcttcg ggaatgccgt
    10141 gcccgcgaat tttctccgag ccgtagcgtg ccatcaggcc gatcatcagc ccgccgataa
    10201 tcggcacagc gatcacgaga tagccgaggt ggtggtcaac cggagattgc aacaccgagg
    10261 agaactgccc gtagaacgcc aggttcgtaa tcaccgctat cagccacacc aaccccgtgg
    10321 cggtaagtgc gctcagaagg ccgatgatca gcgccattaa cgcgagcacg atcacacgcc
    10381 ggtcgcgcgt aaagtcggca aggcggtctt gagttgtttc ggttgtgtcg ttcatggtgt
    10441 ctcatttcac gcgacaaagc acgtcgtcgt taaagttgat atttctcttc gccaacagaa
    10501 tccgacggtg gatcggcggg gagatggagg gcggtgcgct caggcaacca gcggctcaat
    10561 actaaaatat atcgtgttac gatataaatt tcaaagccag caatcgaagg cggtgcctgc
    10621 gaatgaacgg tagactccga tactcagcct tgacctctag gtgacctgaa acaaatcaca
    10681 cgccgatgcg catcggcgtg cgctcgctct gaatgcttgt tgcgcgaagg gtgctctacg
    10741 tgaagtttgc gtcgtccttt gcgccgacgc aaaagaaggc caacgaaatg gcgttggcgt
    10801 aatggtcgtt tatcgaggaa gaaaattcgg cattcggtcg gcgctggcgc tctcgcgtgc
    10861 ttggcagctg cgagaacggt gccccgcgcg gcgcgcgttt ttattcagcc gaatgccgat
    10921 gtatcgaaaa gtctcgcccg ggatcatggg gtcctgggac gagagatccg gtcccgtaag
    10981 gtaaatgttt ctgccgtctc cgtcggaact cggacccatc ggaatggctc cgagttccgg
    11041 tctttgcgct gattggcctc ctggccgttc ccttagcgag tagctgcatg ctcgcgcaat
    11101 cacgcctagc cttcaccagc agattagtat ggtcaggttt ctaattaccc acttgctttc
    11161 aatccgaggc tggctttcgc ggcattcccg cgacggcagg acgctgcccg cttatttcgc
    11221 cccaagcaac ttcgaccaac ttgtgaggtc gtagctccgc aagctgcttg agattccgac
    11281 aatc
//
LOCUS       DASUVS010000043         3147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_233528, whole genome shotgun sequence.
ACCESSION   DASUVS010000043 DASUVS010000000
VERSION     DASUVS010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233528"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(322..813)
                     /locus_tag="VF182_02610"
     CDS             complement(322..813)
                     /locus_tag="VF182_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6435995.1"
                     /translation="MDRRGNGPNPGIGQFRSGSCGESPKWLGGLCALGSFAGALQFSE
                     RFERFEEFARDRRVVAHVHLPVLGGHGHEADGDGAVRVEVLGAILDELPRAAALDIGG
                     GVEDFRLEATRAQAAPVSVGKPQDESVFGGVAWLERVAEAPENAGILVLVFFGEDDES
                     GRG"
     gene            914..2107
                     /locus_tag="VF182_02615"
     CDS             914..2107
                     /locus_tag="VF182_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEX6435996.1"
                     /translation="MSRVTSIFSQILQLYPRLEFEAAVKYHKAERHSRGFSCWGQFIA
                     MLFCQLGRAHSLREITQGLAASEGKLRHLGLQETPKRSTLAYANANRSWELYQTIFEI
                     LYRRCSATASARQQKFSFTHKLLSIDSTVITVCTSLFGWANYKQRKGALKLHLLLDHD
                     GYVPKFAVITDGKESDISVALEMEFEPGTMLVFDRGYIRYGWWAGLSKKGVWFVSRLR
                     ENAKYEVIDSRLLPPDPDPKEKRTRVRADRRIVFPEQKQQKDRPEFRLVEIVTEEGEV
                     LEFITNAHHLEALTVAGVYKDRWQIETFFRSLKQLLKIKTFVGTNENAVRIQIWTALI
                     AMLILRYLKLRASFNWSLSNLLALLRQQLFVHRDLWGWLDKPFEPPPEVADAQPLLWP
                     EMAGN"
     gene            2409..>3147
                     /locus_tag="VF182_02620"
     CDS             2409..>3147
                     /locus_tag="VF182_02620"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX6435997.1"
                     /translation="MSSLRLDSRDGLIKGGAKGPAIVSGNSAQSRLIQMVAHAAQPSM
                     PPGKKLPDDDIAALRAWIDAGAPWPKEGGALEVKAPTWWSFKPPVRPDVPASAKPIDH
                     FVTANLNGLTPSGRASKATLVKRAYYDLHGLPPTFEQSQKFINDTSPDAWDKLIDKLL
                     ASPRYGEKWGRHWLDLVRYGDTAGFEQDPYILDAWRYRDYVIESFNADKPYDRFVKEQ
                     IAGDELWPDDPKSRMGTGYFTVGTNRDM"
BASE COUNT          703 a          954 c          882 g          608 t
ORIGIN      
        1 gctccacaac cccccgcgtc tgcgtccgct cataataccc ccacttgtcc attcacaagc
       61 gatcgcaaac tatatttcgc gtacttacaa ccacttactc aaacaccccg cctccagccg
      121 cactttggcg tgcgatgatc tcggcaggag cgagcgccgc tcatcatcac ccggcgctcg
      181 ctcttctgtt ttttgcaaat cggaactact catatgccat cagaacgaca actcgccgcc
      241 aaccgccgca acggcgccaa aggcggacct aaaaccgacg ccggcaagga acgctgccgg
      301 acaaatagca tcaaacacgg tctaacctcg tccactctcg tcgtcctccc cgaagaacac
      361 gagcacgagt atgccggcgt tctccggggc ttccgcgact cgttccagcc atgcgacccc
      421 gccgaagacg ctctcgtctt gcggcttgcc cacgctcact ggcgcagcct gcgctctcgt
      481 cgcttcgaga cggaaatcct caacaccacc gccaatgtcc agcgccgccg cgcgcggaag
      541 ttcgtcgaga attgccccga gcacctcgac ccgcacggcg ccatcgccgt cggcttcatg
      601 accatgcccg ccgagcactg gcagatgtac atgcgctacg acacggcgat ctcgcgcgaa
      661 ttcttcaaaa cgctcgaagc gctcactaaa ctgcagagcg cccgcaaacg agccgagcgc
      721 acacaacccg cccaaccact tcggactttc gccgcaggag ccggagcgga actgtccgat
      781 tccgggattg ggtccgtttc cacgcctgtc caaattaggt ttttccgaga gaggaaaagc
      841 aaaagcggag cttctgatag ggtgattagg ttccagcaag aactaaattc accgaaccgg
      901 agtggctccg ctcatgtcca gggtaacaag catcttcagc cagattctgc agctgtatcc
      961 acgattggag tttgaagcgg cagtcaagta ccacaaggcg gaacgccaca gccgcgggtt
     1021 tagctgctgg ggacagttca ttgcgatgct gttctgtcag ctgggacggg cgcattcgct
     1081 gcgcgagatc actcagggtc tggcagccag tgaagggaag ctacgacatc tgggtttgca
     1141 ggaaacgccg aagcgctcca ccttggccta cgccaatgcc aatcggtcgt gggagcttta
     1201 tcagacgatc ttcgaaattc tgtatcgccg gtgtagcgcg acggccagcg cgcgccaaca
     1261 gaagttctcc ttcactcaca aactgctttc catcgattcg acggtgatca cggtatgcac
     1321 ctcgctgttt gggtgggcca actacaagca gcgcaaagga gcgctgaaat tgcatctgct
     1381 actggaccat gacggctatg tgcccaagtt cgccgtcatc accgacggta aagaatcgga
     1441 tatctcggtg gcattggaga tggagtttga gcccggaacc atgctggtgt ttgatcgcgg
     1501 ctacattcgc tacggttggt gggcgggcct gagcaagaaa ggtgtctggt ttgtgtcacg
     1561 gctgcgcgag aacgctaaat atgaagtgat cgatagccgg ctgttgccgc ctgaccccga
     1621 cccgaaagag aaacgaacga gagtgagggc ggaccgtcgg attgtgtttc cggaacaaaa
     1681 gcagcaaaag gatcggccgg agtttcggct ggtggagatc gtcaccgagg agggcgaggt
     1741 gctggagttc atcaccaatg ctcatcatct agaggcactc acggtggcgg gagtatacaa
     1801 agaccgctgg caaatcgaga ccttcttccg cagcctcaag cagctattga agattaagac
     1861 ctttgttggg actaacgaga atgccgttcg cattcagatc tggacggccc tgattgcgat
     1921 gctgatcctg cgctacctga agttgcgtgc cagcttcaac tggagcttat ccaatctgct
     1981 ggcgctgtta aggcagcagc tgtttgtgca ccgcgatctc tgggggtggc tcgacaaacc
     2041 atttgaacca ccgccggagg tggcggacgc gcaaccgttg ttgtggccgg agatggcggg
     2101 caactaagtt ggacagcagc cccagcgaaa aaaggaccct ggatcacgaa aagccaaaaa
     2161 tgcacgagca actgcaatgg aatcaacatc gccaaggagg gtcacccggc taatttggac
     2221 agcattggtc cgtttcgcag aacggcggta tcgcatcaac tgtcatttcg gcgataataa
     2281 tcactaccat gagtcggctc ggactcctcg ctattcttgc gtctgcgctg tttgcccagg
     2341 gcgattccca gcgtggaatc gaagttctcc gcgcgaactg tacatcgtgc cacgggtcgg
     2401 cgatgcagat gtcgagcctt cggcttgact cccgcgatgg gttgatcaaa ggcggagcca
     2461 aagggccggc gatcgtcagc ggaaactccg cccaaagccg gctgattcaa atggtggcgc
     2521 atgccgctca gccttcgatg ccgcccggca agaagctgcc ggacgacgat atcgccgcgc
     2581 ttcgcgcctg gatcgacgcg ggggcgccct ggcccaagga aggcggcgct ctggaagtca
     2641 aggctccgac gtggtggtcc ttcaaaccgc ccgttcggcc ggatgttccc gcgtccgcga
     2701 agccgatcga tcactttgtc actgcaaacc tcaacggcct gacaccttct ggccgagcct
     2761 ccaaagcgac gctggtcaag cgagcctact acgacttgca cggactgccg cccaccttcg
     2821 agcagtcgca gaaattcatc aacgacacct ctccggacgc atgggacaag ctaatcgaca
     2881 agttactggc gtccccgcga tatggcgaga aatggggccg ccattggctt gatctcgtcc
     2941 gttatggaga tactgcggga ttcgagcaag acccgtacat cctcgacgcg tggcgctatc
     3001 gcgactacgt gatcgaaagc ttcaatgccg acaagccata cgaccggttt gtcaaagagc
     3061 agatcgccgg cgacgagctt tggcccgacg atccgaagtc gcgcatgggc accggttact
     3121 tcaccgtcgg aaccaaccgc gacatgc
//
LOCUS       DASUVS010000044        10082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1762967, whole genome shotgun sequence.
ACCESSION   DASUVS010000044 DASUVS010000000
VERSION     DASUVS010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10082
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762967"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="VF182_02625"
     CDS             complement(<1..1318)
                     /locus_tag="VF182_02625"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6435998.1"
                     /translation="METHSQNYSYYLLALTLLVFFTGLGGRDLWAPVEPRYGEIVRIM
                     FAKGEWVVPTVNGDFYTDKPILYFWLALIASKIAGGVNEWTVRLPSALAGIGFILGTY
                     FTGRDFFSPRVGLIAGAVLATSVRVIWEARWAHIDMLFGFLFLLTIYFGARSLLSKGG
                     KREILWAYVFMGLAVLAKGLIGIVLPALLLAAFAIVRRDWRLIVDAKLELGIPIFLVI
                     AAPWFYLVNSATDGKWLADFIYIHHVERYIDGMGHRQPFYYYLTTLPADFMPWTIFAI
                     PAVVAYFPCRRLMQRPIPLFFSLCFLSVFLFFSISDTKRDLYLLPLMPTLALLVGNYI
                     EDLTEGRSESALYRWLSQFYFGAVAILGVALPIVAWTVRRETFWISLPVALVLLIGGV
                     VTVILIRKRRPLQAVTAVAWLIVALMVSGSLWILPYVNQFKSHRPFS"
     gene            complement(1573..2352)
                     /locus_tag="VF182_02630"
     CDS             complement(1573..2352)
                     /locus_tag="VF182_02630"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX6435999.1"
                     /translation="MDGMVPATRLAIEKGSRKARSLSIAVGNFFFQHRNRLFPLIFLL
                     AALTLRPSIMFGSPLADRLLAASGMIIVLFGELVRLLTIGFEYIHRGGKEGRVYAGRL
                     VQGGVFGLVRNPMYIGNVLIAVGMTVYLRSPLAYAVLIPLFMFLYQALIAAEEAYLCD
                     RFGSDYEEYCAKVNRFLPRLEHIPEAFSGMRFDWRRSVRKDLGTVVGLTMGLILIPLW
                     RTYHLDGWATMLSRIPFSLGLSLALTLGYLVLLRLKKSNRL"
     gene            complement(2357..3082)
                     /gene="cmoA"
                     /locus_tag="VF182_02635"
     CDS             complement(2357..3082)
                     /gene="cmoA"
                     /locus_tag="VF182_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HEX6436000.1"
                     /translation="MAHDYLFSEKRDVIRDFDFGKDTAVVFDDMLDRSVPFYSEVQRM
                     IGEIALDYAVEGTQLYDLGCSTCNAFVKLDPLLPPNIRFVGVDFSNEMLDRAREKIKQ
                     HGMRRDYQLICADLNEDIEITNASVVIINLTLQFIRPVRRHRVISNIARGMTPGSCLL
                     VVEKVLSKDPKINRSFIKYYYDFKQRNGYSQLEIAQKREALENVLIPYRLEEDFELFL
                     SSGFSFCDLFFKWYNFCGMIAVK"
     gene            complement(3108..4982)
                     /locus_tag="VF182_02640"
     CDS             complement(3108..4982)
                     /locus_tag="VF182_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6436001.1"
                     /translation="MPNHNSNREGLGILQLNRRLSSRLFPGSLAPKKFVVLGTLGGFL
                     GNIITAGINGVRLLRAAAEAHAPVLHIILLSALCGFLYFPYLGSTAFFDKGEPREALA
                     VQDIVRRGEWLFPLKRATDIPSKPPLFHWSAAVSSKIIGRLDEFTIRFPSALYATLGV
                     LLVYVFARKLFGAQVALLGGAILGTSLVYSNQAVTARVDMTLCFFVTLSLVLFYSLYQ
                     RFLTGGLWYYLFYLTLGIGVLAKGPLGILLPALVIASFIALKKRWDLFFKFSCHPGLI
                     LTLLVAVGWYAIALIRGGEGFMDRQLLQENIERFYGGSGHSHPLYYYIPYLFFGGLPW
                     SLFLAFLVWDLFKKRSSLGDDILFLSLWALVMFAFFSISMGKRPVYLLPLYPALSMLM
                     AVWLYHYKALSPRTFGFRSVAMLAGVVGVLLLIISLGALWNPDAGWFFASVESFLKSK
                     DRANLILAEDSFAGFGRSFTLLALIGALLWFSFARWLWAGRPMTATPPLILISFLVAF
                     VSRGMVMPVLAEAKSYRSFIEQVDQRLKPGDELYLFGEFFNSDSVVFYHGGPIETIDQ
                     LPPGLATNIGSGNNYIIMAKRSWEEIKRYNQSLPGPILSSGSTGPEGDAPLVLIQMRG
                     "
     gene            complement(4982..6766)
                     /locus_tag="VF182_02645"
     CDS             complement(4982..6766)
                     /locus_tag="VF182_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6436002.1"
                     /translation="MDIATGYSPVDRVTSAKSFDPHTWWFIAISLFCLVVLFSDLGGA
                     ALFEPDEGRNAEVAREVLLLDDWITPHYDFIPRLDKPAFFFGLVALAFKVFGLSEWAA
                     RLPSVLAALGCLSLTYIVARSVFGRWAALWSVLILLTSVEFFALSRVVILDMVLTMFI
                     TLSLVCFWLGHRATGARRKFLVLLMYASMGAATLVKGPIGFVLPAAVIFFYLLLSGKW
                     ALLREMEVSLGVPLFLILAGSWYVAVEFRNPGYLHHFLYEENFARFTSTQFNRSGPWY
                     YFFMVLAAGFFPWTLLLPMTLADFRKRPPAEGHLFLSLWMALPFIVFSLSASKLPHYI
                     LPLYPPLAILVGASVAKAYTAAPSAKTSWVLSLPAITFFLVALLPALLFLPSDFLPKK
                     IQPQIYAVLPRTSVLFTAAAAALLLGLAAYKKGLWRREFYVYGSTAVGFALFILVAEP
                     ITASVSLTRSSKQLAEAAASVIHQGDQLVLYEKYLSSLPFYLRIQQPIWVVWSGDKSK
                     VLGSDYIAKKRPEPAAGYGKVLYIYEEFTNLSRASKSRLVVFVDDRAFDALPIAGGAP
                     TKLLDVGGISLVTNHPEPRLLSLSRWTQ"
     gene            complement(7510..8901)
                     /locus_tag="VF182_02650"
     CDS             complement(7510..8901)
                     /locus_tag="VF182_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6436003.1"
                     /translation="MPSSSRKRFHKTLGFRLTFWYSAIFILSSLTLSVVSFAFVFSTM
                     RDNRKAIQLKLAEYRSLADTDGVQALAKTLAEQRNPSRRTTFFVRVIAPGNKTVLLSN
                     PRLWEEFDFLRFDDRQPEYQWSYFPSKRDTGLLEVTSAQLANDYILEVGKSIQDREDI
                     LQRFRDTLFATMIPMILIGLGGGAFLAYRALRPIRALIGTTRSIVETGRMDLRVPESK
                     HQDELNELVRLFNNMLERIERLIRGMKDALDNVAHDLRTPMTRLRGTAEMCLQAPAKA
                     EHYKESLATCVEESDRILTLLNSIMDVSEAETGIMRLKLETVNLTKLIREMVELYHYV
                     AEDSDVVVSVDCPEDFHLTADYIRMRQVIANLLDNAIKYNRVGGRVIISGRVEGPHAV
                     VSFRDTGIGIPAEEVAVIWDRLYRGDKSRSQPGLGLGLSVVKAIVRAHRGDVEVRSEP
                     GIGSEFIIRLPAA"
     gene            complement(8882..9556)
                     /locus_tag="VF182_02655"
     CDS             complement(8882..9556)
                     /locus_tag="VF182_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005983179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6436004.1"
                     /translation="MRILVVEDDKKIASLVVRGMKQAGFAVDHAENGEDALHLALSEP
                     YDAAIIDVMLPKLDGLTIIERMRERKILTPVIVLSAKRSVDDRVRGLQTGGDDYLVKP
                     FSFAELLARVHALIRRASHEAEPSRLIIGELTLNLLTREVTRAGKKIDLQPREFSLLE
                     YLMRNSGRVVSKTMIIEHVWGYNFDPQTNVVDVLVSRLRNKVDRDFEDQLIHTHRGVG
                     YALKLS"
BASE COUNT         2675 a         2696 c         2642 g         2069 t
ORIGIN      
        1 gtgaaaacgg ccggtgcgac ttgaactgat tcacgtaggg caggatccag agcgagccgg
       61 ataccatgag cgcgacgatg agccatgcta cggcggtaac cgcctgcaaa ggccggcgtt
      121 tgcgaatcaa aattaccgta acaactccac cgataagcaa aaccagcgcg acgggcaggc
      181 ttatccagaa ggtttccctg cgaacagtcc aggcgacgat tggcagcgct acaccgagga
      241 tagccacggc gccgaagtaa aattggctca accatcgata aagtgcgctc tccgatcgac
      301 cttccgtcaa atcttcgatg tagttgccga ccagtaaagc caaggtgggc atcagcggca
      361 ggagatataa gtcacgctta gtatcggaga tgctgaagaa cagaaaaacc gacaggaagc
      421 atagcgaaaa aaacagcggt atgggccgct gcataagtcg ccggcaagga aagtaagcga
      481 ccacggcggg aatggcgaag atggtccacg gcataaagtc cgccggcaaa gtcgtcaaat
      541 aataatagaa gggctgccga tggcccatcc catcgatata gcgttcgaca tgatgaatgt
      601 aaataaaatc cgcaagccat tttccatcgg tcgcgctgtt gaccagataa aaccatggcg
      661 ccgcgatcac gaggaatatc ggaatgccga gttccagctt tgcatcgacg atgaggcgcc
      721 aatcacgccg gacaatcgca aatgccgcga gcaaaagcgc cggcaagaca ataccgatca
      781 accctttggc gagaaccgcc agacccataa agacgtaggc ccagagaatc tcgcgttttc
      841 cacccttact tagaagagaa cgagcgccga agtaaatcgt caggagaaac aagaatccaa
      901 acagcatgtc gatgtgcgcc cagcgcgctt cccatatgac ccgcaccgaa gtcgccaaaa
      961 ccgcgcccgc gatcaatccg actcgcggac tgaagaagtc gcggccggta aaataggttc
     1021 ccaagataaa gccgatgccg gcaagagcgg atggcagacg cacggtccat tcgtttaccc
     1081 cgccggcgat tttcgatgca atcagcgcca gccagaaata aaggatgggc ttgtcggtat
     1141 agaaatcgcc attgaccgtc ggtaccaccc attcaccttt ggcaaacatg atccggacga
     1201 tttcgccata gcgcggctcc acaggcgccc ataaatcacg gccgcccaat cctgtgaaaa
     1261 acaccaacag ggttaaagca agcaagtagt atgagtagtt ctgactgtgt gtttccaagc
     1321 tgcgcaccct gtgtgttgtg atagcgataa gcaaatatcg gagaagtcag cggacaaacg
     1381 gcgcttcggg ccggcaactg cttggctgaa tggtctggcg cctatccatc atttctctcc
     1441 cacccgatcg gagactgccc tctatgtcga ttccgcagcg tcgtatcttc gatgacccgg
     1501 cataaaggtg atgtcgacgc ggcagcaacg acctcggccg cggctttctt cttttctccg
     1561 gccgagaact ttttataagc gattgctctt tttgagcctg agaagaacca ggtagcccag
     1621 cgttagagca agagataacc ccagggagaa aggaatcctc gatagcatcg tcgcccaacc
     1681 atcaaggtga taagttcgcc agagcggtat caggatcagc cccatggtca atccgacaac
     1741 cgtgcccaaa tctttcctga cactgcgtct ccaatcaaag cgcatgcctg aaaacgcctc
     1801 gggaatgtgc tccaaccgcg gtagaaagcg attgaccttc gcgcaatatt cttcgtaatc
     1861 gctgccgaac ctgtcacaca ggtaagcttc ctccgctgcg atcaaggcct gatacaggaa
     1921 cataaagagc gggattagaa ctgcatatgc gagcggcgac cttagatata ctgtcatgcc
     1981 gaccgcaatc agcacgttgc cgatatacat cggattgcgc accaggccga acacccctcc
     2041 ctgaaccagc cgccctgcat acacgcgtcc ttctttgccg ccacgatgaa tatattcgaa
     2101 gccgatggtt agcagccgaa caagttctcc gaacaacacg atgatcatgc cgctcgcggc
     2161 gagtaaccga tcggcaagcg ggcttccgaa catgatgctc ggcctgagag tcagagccgc
     2221 gagaagaaag ataagcggaa aaagacgatt gcggtgctgg aagaagaagt tgcctaccgc
     2281 gatacttaac gaacgcgcct ttcgagaacc cttttctatc gctaaccggg tcgccggaac
     2341 catgccatcc ataaaattat ttgaccgcaa tcatcccgca gaagttatac cacttgaaga
     2401 aaagatcgca gaaactgaat ccgctgctca gaaacagttc gaaatcctct tcgagcctgt
     2461 agggtattag aacgttttcc agggcttcgc gtttctgggc gatttcgagc tggctgtaac
     2521 cattgcgctg tttgaaatcg taatagtact tgatgaacga gcgattgatc ttaggatctt
     2581 tgctgagcac cttttcgaca accaggaggc aactgcccgg agtcattcct ctagcgatat
     2641 tgctgatgac ccgatggcgg cgcacgggcc ggataaattg cagcgtcagg ttgataatga
     2701 ccaccgaggc gttggtgatt tcaatgtcct cgttaagatc ggcgcaaatg agctggtaat
     2761 ctcgcctcat cccgtgctgc tttatctttt cccgcgcacg atcgagcatc tcattggaaa
     2821 agtcgacgcc gacaaagcgg atgttcggtg gaagaagcgg gtctaacttt acaaacgcgt
     2881 tgcaggtcga gcagccaaga tcgtaaagct gggtcccttc caccgcgtag tccagggcga
     2941 tttctccgat catgcgctgc acttcggaat agaacggcac cgaacggtcc agcatgtcat
     3001 caaaaacgac cgccgtatct ttgccgaaat cgaaatctct gattacgtct cttttctcgc
     3061 tgaacaaata atcgtgagcc ataaagtact ctcctgataa tttgtattta tcctcgcatc
     3121 tgaatcagaa ccaaaggcgc gtcgccttcc ggacccgtac ttccgcttga taaaatcggc
     3181 ccggggagac tttggttata gcgtttaatt tcttcccagc ttcgtttggc catgatgatg
     3241 tagttgttac cggagccaat gttagttgcg agccctggcg gtaattggtc gatcgtttcg
     3301 atgggaccgc catgataaaa gaccacagaa tcgctattga agaattctcc aaataggtag
     3361 agttcgtcgc ccggcttgag ccgctgatcc acctgttcga tgaacgagcg atagcttttt
     3421 gcttctgcga gaaccggcat gaccataccc cgacttacga acgcaaccag aaacgatatc
     3481 aatatcagtg gcggcgtcgc cgtcatcggc cgaccagccc acagccagcg agcgaatgaa
     3541 aaccaaagaa gcgcacctat cagcgcgaga agcgtaaatg accggccgaa gccggcgaaa
     3601 gagtcctcgg caaggatcag attggcgcgg tctttcgatt tgagaaaact ctcgacggac
     3661 gcgaagaacc acccggcatc agggttccac aaggcgccca aactgatgat cagcagtaga
     3721 acaccgacta ctccggcgag catcgcaacc gagcgaaaac cgaaagttcg cggcgatagc
     3781 gctttgtagt gatacaacca aactgccatt aacatggaca gagcgggata cagtggcagg
     3841 agatacaccg ggcgcttgcc catcgagatg gaaaaaaacg cgaacatgac cagcgcccag
     3901 aggctcaaaa aaagaatatc atcgccaagc gaagatctct ttttgaacaa atcccaaacc
     3961 aggaatgcta agaacagact ccatggcagc ccgccaaaaa aaagatatgg aatgtagtag
     4021 taaagcggat ggctatggcc cgaaccgcca tagaatcgct cgatattttc ctggagaagt
     4081 tggcgatcca taaatccttc tccaccacgg ataagcgcga ttgcatacca gccgacagct
     4141 acgagcaacg tcaaaatcag cccgggatga caagagaact tgaaaaagag gtcccatctt
     4201 ttcttgagcg ctataaacga agcgatgaca agcgcaggca gcaatatacc cagcggccct
     4261 tttgccagaa cgcctatgcc aagcgtcaga tagaaaagat agtaccagag tccgccggtc
     4321 aggaagcgct gataaagcga atagaaaagc accaggctga gggtcacaaa aaaacacaag
     4381 gtcatatcga cacgcgcagt gaccgcctgg ttggaataga ccaggctcgt ccccagaatc
     4441 gcgccgccga gaagagcgac ctgtgcccca aaaagtttgc gggcaaaaac gtagacgagc
     4501 aaaacaccca aggttgcgta taaggccgag ggaaagcgga ttgtgaattc gtcaagccgg
     4561 ccgatgattt tggatgaaac cgccgccgac caatgaaata gcggtggctt ggacggaatg
     4621 tctgttgccc tcttcagagg aaacagccat tcgccgcgtc gcacaatatc ctgcacggct
     4681 agcgcttctc ttggctcgcc cttgtcgaag aaggcagtac tgccgagata cggaaagtac
     4741 aagaatccgc acaacgcgct gaggagaatg atatggagaa caggagcgtg cgcttcggcc
     4801 gccgcgcgta aaagcctaac gccgttgatg ccggcagtga ttatattccc taggaacccg
     4861 ccgagggtcc cgagtacgac aaattttttg ggcgccagac ttcccggaaa caaccgcgac
     4921 gagagccgcc tgtttaactg taagatcccg agaccctctc gattggaatt atgattcggc
     4981 actactgggt ccacctgctc aaagaaagga gcctaggctc gggatgatta gtcaccagag
     5041 agatgccgcc aacgtctaag agcttcgtgg gtgcgccacc cgctatcggc agcgcgtcga
     5101 aggcgcgatc gtcgacaaac acaaccagcc gacttttcga cgctcgcgac agatttgtga
     5161 attcctcata gatgtaaagc actttcccat atccggcggc cggttcgggc cgcttcttag
     5221 caatgtaatc gctgcccagg actttgctct tgtctcccga ccaaaccacc caaatgggtt
     5281 gttggatacg aagataaaaa ggcaggctgc tcaaatattt ttcatatagc accaactgat
     5341 ctccctggtg gatcactgaa gctgcggcct cggccagttg cttggacgat cgagtcagag
     5401 acactgaagc cgtgatcggt tccgcaacca ggatgaatag ggcgaaaccc acagcagtgc
     5461 tcccatagac ataaaattcc ctgcgccaaa gtcctttctt gtaagctgcg aggcccaaca
     5521 ggagcgccgc cgccgcggca gtaaacagaa cggaagttct tggcagaacg gcataaatct
     5581 ggggttgaat cttcttcggc aaaaaatcag acggcagaaa caatagagcc ggtagcaagg
     5641 caaccaagaa aaaagtaatg gccggaagcg aaagaaccca gctcgttttt gcggaaggtg
     5701 cggcggtata agccttggcg accgaggctc ccaccaaaat agccagcggg ggatataagg
     5761 gcagaatgta atggggcaac ttagatgctg aaagcgaaaa aacaataaat ggcaaagcca
     5821 tccacaagct cagaaacagg tgtccttccg ctggcgggcg cttacggaaa tcggccagtg
     5881 tcatcggaag cagaagcgtc cacgggaaaa acccggcggc taaaaccata aagaagtaat
     5941 accagggtcc gctgcgattg aattgcgtgg aggtaaaccg agcgaagttt tcctcataca
     6001 agaagtggtg caggtatcca ggattacgga actcgaccgc aacataccag gacccggcca
     6061 aaatcagaaa gagcggtacg cctagcgata cctccatttc tcgcagcaga gcccattttc
     6121 ctgataacag aagatagaaa aagatcaccg cagccggaag gacaaaaccg atcgggcctt
     6181 ttaccagcgt cgctgccccc atggaagcat acattaggag aaccaggaac ttccgccggg
     6241 cgccggtggc gcgatgaccc aaccaaaaac aaaccagaga aagggttata aacattgtca
     6301 gcaccatgtc gaggatcacc actcgggaga gcgcaaaaaa ctcgacactg gtcagcagaa
     6361 tcaacacgct ccagagcgcc gcccatcgtc caaaaacgga tcgcgcaaca atataggtga
     6421 gtgaaagaca gccgagcgca gccaacaccg aaggcaggcg ggccgcccat tcggataagc
     6481 cgaagacctt gaaggcaaga gctaccagcc caaaaaagaa agcgggctta tccaggcgtg
     6541 gaatgaagtc gtaatgcggc gtaatccaat cgtcaagcag caggacctct ctggcaactt
     6601 ccgcgtttct cccttcgtcc ggctcgaaca acgccgcgcc tcccaaatca gagaacagaa
     6661 caaccagaca aaagagagag atcgcgataa accaccacgt atgggggtca aagctcttgg
     6721 cgctcgtgac tcgatcaacg ggagaatatc cggtagctat atccatacga tgtcactcgg
     6781 tgagattcca atgagtttta gtccaagatg ttgtcttctg ttttcttcat gcggtgcgaa
     6841 accggagcac gcctcgagtt aaaggaagtt atgcgccggt atcatcgtta ggcgctcggt
     6901 gttcataggg ttggctcgtc catgtaatcg gtaatcgatg ttagttcgtg accccagcat
     6961 tcttgtcatc cgccgcatct gaatagttca ccttgacttc tcgccccacc aaagctctga
     7021 gtgcgacccg ttcttcttga cgggattgcg gagcattgaa cttcccgagc tcagcctcgc
     7081 tgttctcgcc cgggtaggtc catcgagcct acgacgattt cattgcagca atggctgcac
     7141 tctataatct gcctggtttg cttaatcgaa ataccgaata gattaacttc tcggcacatc
     7201 tccgatgtat ggcaatgacc tttcaagcgg cagccaggac attcatagtc aggagccggc
     7261 tgcgtgaagt cgcaacgatg ccatccaagg agtgcgagct ttttgccgtt gttattgcta
     7321 atcatcgaat cgtacgtgat cgctcgccag tcatgggttc gattcacgtt cactcgtact
     7381 gagtaaatcc gttcccaccg gtcatactgc gactggatcg aagatagtgc agaaaccttg
     7441 cccgaacatg tcggcaagat tacagttttg taaggttgga aaggagacga taaacagtag
     7501 gcggggagat caagcggcag gtaggcgaat gatgaactcg gaacctatgc caggttcgct
     7561 gcgaacctcg acatctcctc gatgggcgcg gacgatggct ttgacgacgc tcaagcccag
     7621 gccgagtccg ggctgtgagc ggcttttatc gccccggtag agacggtccc atatcaccgc
     7681 aacttcctcg gcggggatgc caatgcctgt gtcccgaaag cttacaaccg cgtgaggtcc
     7741 ttcgacacgg ccgctgatta tgacgcggcc accgacccgg ttgtatttaa tcgcgttatc
     7801 caacagattg gcgatcactt gccgcatacg gatgtagtcg gcggtgaggt ggaaatcttc
     7861 ggggcagtcc accgatacaa cgacatcgct atcctccgcg acgtaatggt acaactccac
     7921 catctcacga atgagcttgg taagattcac ggtttcgagc ttcagccgca tgatccccgt
     7981 ttcggcctcc gacacgtcca tgatgctgtt tagcaacgtg aggatccgat ccgattcctc
     8041 gacgcacgtt gccaacgact ctttgtagtg ctcggccttc gccggcgctt gtaagcacat
     8101 ctcggctgtg cctcgcaacc gggtcatagg agtacgaaga tcgtgggcga cattatccag
     8161 cgcgtctttc attcctctga tcagccgttc gattctctcg agcatattgt taaaaagtcg
     8221 aaccagttcg ttcaattcgt cctgatgctt gctctcaggc acacggagat ccatccggcc
     8281 ggtctcgacg atagaacgtg tcgtaccgat aagcgcgcga atcgggcgca aggcgcgata
     8341 cgccaaaaaa gctcctccgc ccaagccaat caggatcatc ggtatcatcg tcgcgaacag
     8401 cgtatcccgg aatcgttgca gaatgtcttc gcgatcttga atgctctttc cgacctccaa
     8461 gatataatcg ttggcgagtt gcgctgatgt gacttccagg agacctgtgt cgcgtttgga
     8521 aggaaaatac gaccattggt attcgggctg ccgatcgtcg aaacgcagga aatcgaactc
     8581 ctcccaaagc cgcgggttac tcagcaatac ggtcttattg ccgggagcga taacgcggac
     8641 gaagaaagtg gtgcgccggc tgggattgcg ttgctcggcg agagtcttag ccaatgcctg
     8701 aactccgtcg gtgtcagcta gagaacggta ttccgcgagc ttcaactgaa tcgccttgcg
     8761 attatcgcgc atggttgaga agacgaaggc gaaagaaacg acggagagag tcaaagagct
     8821 caggataaat atcgccgaat accagaacgt aaggcgaaag ccgagcgtct tatgaaagcg
     8881 cttacgagag cttgagggca tagccgactc cgcggtgagt gtgaatcagt tggtcttcga
     8941 aatcacggtc tactttgttg cgcagtctgg atacaagcac gtcgacaacg ttggtttgag
     9001 ggtcgaaatt gtagccccac acatgctcga tgatcatggt cttggaaaca actctcccgc
     9061 tattccgcat caggtattcg agcaaagaaa actcgcgcgg ttgcaagtcg atcttcttac
     9121 ccgctcgggt aacctcccga gtcaaaaggt tcaatgtgag ctccccgatg attaatcggg
     9181 acggctcggc ttcgtgactt gcgcggcgaa tcagcgcatg cacgcgggct agaagttcgg
     9241 cgaacgagaa tggtttgaca agataatcgt ctccacccgt ctggagcccg cgcacccgat
     9301 cgtcaaccga gcgcttcgcg cttaatacaa tcaccggcgt caggattttt ctttcgcgca
     9361 tgcgttcgat gatagtcaac ccgtcgagct taggcagcat cacatcgatt atcgcagcgt
     9421 catacggttc cgaaagagcg aggtgaagcg cgtcttcgcc gttttcggca tgatccacgg
     9481 caaatcctgc ctgtttcata cccctcacaa ccaacgaggc gatcttctta tcgtcttcaa
     9541 ccaccaaaat tcgcatggga ttgccggagc taaatcgttc tgtccatcgt tatgctgacc
     9601 gggaggcaaa ctcaaaccag tgtgtcgtgg cagcggtgga gaaaagtttg tctgctaatt
     9661 gtcgctagcc ttcaagaatg taatgtctgt gaaagggaat ggcaacaatt cgagtttatg
     9721 cttaatgcgc tcaaggcagc ggattaagag aatggcaccg cagagacaca gagtgcgcag
     9781 ggacaataat cattcgcacc ggcgtccgtc cgcgcctcgg cggtgaaaga tgagagcgga
     9841 ttggaggcaa ctggctttat aggctcgggg tatagcaaag attaaggtcg ttacatacgg
     9901 taaaagatga aactgctgat gaagattttt atcagcttgg caatgttagc tgtggcgctg
     9961 cgcctggtcg acatcaatga gctgaagcaa agcctgttaa gcattccacc gcggactctg
    10021 ggcgcggtgg tggttatctt ttttttcggc cagctactga gttcctacaa gtggtggctc
    10081 ct
//
LOCUS       DASUVS010000045        11731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1108050, whole genome shotgun sequence.
ACCESSION   DASUVS010000045 DASUVS010000000
VERSION     DASUVS010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11731
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108050"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VF182_02660"
     CDS             complement(<1..545)
                     /locus_tag="VF182_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6436005.1"
                     /translation="MKLKFCLFILVICFVSISRARAQDPQLVEAARKEGKVVWHTSLA
                     LPSSTAISHYFQGKYKGIEVEVHRNGSQRVLQRFMQESTAGLKNADIVHTSDAGHFEL
                     LKDKGLLLKFTPQSVAGFPDGFKDKTGFYYGMRATLSVIAHNPKIVAEKDAPQTWKDL
                     LNPKWAGKMVTAHPGYSGIIMT"
     gene            complement(597..1967)
                     /locus_tag="VF182_02665"
     CDS             complement(597..1967)
                     /locus_tag="VF182_02665"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6436006.1"
                     /translation="MEPYSQAIGRFVHGLSIGDIPPSIQKKAKLVYLDTLGVALASST
                     MDFGGMVTRVAQALGGAPNSRLIGCANKVAAANAVLANGTLAHGLDYDDTLEEAIVHT
                     GCCAGMTALAVGEEVGADGRSVLEAAVAGTEVMCKIGIVAPGKFHARGFHPTALCSTF
                     GAAAAAGKLYGLELSQWTDAFGLCGSQSSGIIEYLADGTWTKRMHPGWAAHGGVIATL
                     LAREGFRGPGKVFEGTHGFFQSFGGPAEYRFEKLLELGKIWETPRLTFKSYPCGSISH
                     PYMDCALKLKQKYAAAPEQIVEVVCRTAQGPVPRLWEPLADKQRPASSYGAKFSLPYC
                     IAVMLIRGRAGLQEFTDNAIRDPSVLALAKKVRYELDPSIDYPRHFSGHVKMRLLDGR
                     ILEENQPHPRGGLEDPLPPEEIESKFRANAQLRLPPARVDQIMAAVNGLEELPKIASL
                     TELLKP"
     gene            complement(1943..3412)
                     /locus_tag="VF182_02670"
     CDS             complement(1943..3412)
                     /locus_tag="VF182_02670"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6436007.1"
                     /translation="MGLTQEVANYVFKASYKDMPTEVIPLARGFILDGLGVALAGSTD
                     ECSRIVQAHVREFGGREECAVLGTAFSAPAAKAALANGVAGHAMDYDDTQLPTSKEAV
                     YGLLTHPTTPVLAAILAVGEKQKISGAEFLLAYILGVEIECRIADSINPRHYQSGFHS
                     TATIGGLGAVMAVGKILRLKENILIQALGICASMASGLRENFGTMTKPLHAGRAAENG
                     VTAALLARSGFTAASNILEAQRGFYRAMGGGYDESKIAGRFGRPYFMQEPGISIKPYP
                     SGSLSHPAQDLILDLVKEHDLHASDIESLEVGTNSNVPNALIYPMPKTALEAKFSIPF
                     CMAIAVLERKAGIAQFVDGKVRQKKIVELMKRVRLYVDDELEGLGFDQVRSRIRIKLH
                     DGRTIEGRYDVARGHPQKPMSWTELAEKFKECASLALPAENAAHVIALVARLEELKSL
                     NSLIGALRSSKARKKIPRRFMQASLKGSKEHKWNRTRKL"
     regulatory      3673..3782
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3840..4601
                     /locus_tag="VF182_02675"
     CDS             3840..4601
                     /locus_tag="VF182_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEX6436008.1"
                     /translation="MSLSLTTEQIERYSRQILVPDFGGKGQIRLRQGRVLIIGAGGLG
                     SPSALYLAAAGVGTLGLIDADDVELSNLQRQILHATADLGKAKVESGKETLTQLNPDV
                     DVIAYPARLGSDNIDGLFLQYQFIIDGSDNFETKFLVNDAAVRLRKAYSHAGIVRWQG
                     QTMTVISGRTACYRCLFKEPPPPAEILNCQQSGIVGAVAGTIGSIQATEAIKYLLGFE
                     EGLLTDRLLTYDARAMKFHQVQVTKDPGCHACSGM"
     gene            4657..5916
                     /gene="thrC"
                     /locus_tag="VF182_02680"
     CDS             4657..5916
                     /gene="thrC"
                     /locus_tag="VF182_02680"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX6436009.1"
                     /translation="MSFVKGLQCRECGQDYPKEPLHVCETCFGPLEIVYDYDAIGKNI
                     SREKIAAREKNLWRYRELLPIDGEPQVGMYSGFTPLVKAHRLAEALGVKELYIKDDSV
                     NHPTFSYKDRVVSVAISKAIEFGFETVSCASTGNLANSVAAHAAKAGLNCYVFVPDGL
                     EQGKIIGSAIYGPKTIAIEGNYDDVNRLCSEIGDKYGWAFVNVNLRPYYSEGAKTHAY
                     EVAEQLGWKLPKHIVVASAGGTILPKLAKAFKELVKVGLVQDTGCKIYSAQAAGCAPI
                     IKALHNGTDLISPVKPNTIASSIAIGNPADGYYVVHAVKDSGGWGDSVTDDEIVEGIK
                     LLARTEGIFTEPAGGTEVAVTRKLIQKGRIPRDESIVISITGNGYKTLESVSRAVEQP
                     YTIKAKLENFDQLYQHLNPSFSKAAVS"
     gene            6007..6285
                     /locus_tag="VF182_02685"
     CDS             6007..6285
                     /locus_tag="VF182_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEX6436010.1"
                     /translation="MSVRVRVPTPLRKFTQGVDEVNAQGNNIRSIVEDLEKNYPGIKE
                     RICDETGKVRRFVNVYVNGDDIRFLQNLETALKEGDNISIVPAIAGGR"
     gene            6363..7304
                     /locus_tag="VF182_02690"
     CDS             6363..7304
                     /locus_tag="VF182_02690"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase"
                     /protein_id="HEX6436011.1"
                     /translation="MGSVLDLIGNTPLLEIRKITEGLPAGVRIFAKLEGFNPGGSVKD
                     RPALRMIQEGIRSGILTGDKVILDSTSGNTGIGLALIGTVLGYRVELVIPANVSIERK
                     QIIQAYGAKITFSDPLEGSDGAIRLSREILRANPDRYFKPDQYFNPMNPQAHYETTGP
                     EVYRQTDGKITHFVAGIGTGGTIMGTGRYLKQMNADIQVIAVEPDDALHGLEGLKHMA
                     SSIVPGIYHEEQLDHKIPVATEDAYAMVYRLSQEEGILVGQSSGAAMFASLKVARKLH
                     SGIIVTVFPDFGDKYLSTSLWVGWRDRMAVGNLKFSI"
     gene            7308..7565
                     /locus_tag="VF182_02695"
     CDS             7308..7565
                     /locus_tag="VF182_02695"
                     /inference="COORDINATES: protein motif:HMM:NF020940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HEX6436012.1"
                     /translation="MKKKREAVRQKIYLTFPRTLVREPVLSSMAKQFDVVFNIRGSTV
                     TAEMGLMALEINGAQAEVNKAVAWLKEKGVTVDSIEKNVVE"
     gene            complement(7566..8027)
                     /locus_tag="VF182_02700"
     CDS             complement(7566..8027)
                     /locus_tag="VF182_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436013.1"
                     /translation="MTRLRNLLKASLLSSLLLLAVSAEGAENGSKPVQVKIGTILASN
                     ESDEFDARLKPMERQLKVMKYRSYRLLKEDSQSVPWNDNATFEIPGGRSLAVSPQDSK
                     DKQIALKVRLTEGTKPLLDTTVRLQGRGYFLLGGPPHEGGALVISISASAQ"
     gene            complement(7984..8526)
                     /locus_tag="VF182_02705"
     CDS             complement(7984..8526)
                     /locus_tag="VF182_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6436014.1"
                     /translation="MSNCETTREILGAWLDGELVPAQAEACRLHVDACAACREERHKL
                     ERLHSTMQTVLSSSASKVQFDPFWQGVQQRIAQQSKWYDELGDWIRGIFTAPRLAWAV
                     PAVIVIVLAGLSIDSFLPNAKLGMQPDNLATVESIDAYGRNVALLHEGETNTTVIWLY
                     QPQESENDSSTEPSESKPSF"
     gene            complement(8519..9076)
                     /locus_tag="VF182_02710"
     CDS             complement(8519..9076)
                     /locus_tag="VF182_02710"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6436015.1"
                     /translation="MELSDWQLVQKCQAGDIASFQELVSRYHQKVYMVIFGLLRNRDD
                     AMEVAQESFFRAYRKITSFHGQSSFYTWLYRIAVNMSIDHQRRQKRSPLEFRESMDEV
                     FEQQNEVAKDPFSDVHDRELREKMLSAIDDLTPEHKAVIVLRTLEGMSYKDIGEILGC
                     SEGTVMSRLHYARKKLQNKLGAFYE"
     gene            9230..10273
                     /locus_tag="VF182_02715"
     CDS             9230..10273
                     /locus_tag="VF182_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX6436016.1"
                     /translation="MLVKCPSCATTYKVADEILTGTAPAFRCSRCKHMFEGDLDASVE
                     AVPLRAESESKIQGEDEGELAFVFEAEDQDPRDDRDQDGINPPPPTHFPPPDPVDSLV
                     EQETQRWSVPAEIKAEEPFTISGDPRSQQIEGKPDRHEDGKRARKPAEEVLPTMPTSN
                     ISSLEPYRDQQTSTLPYLSLFALLILFFSLATAFQYAYPKVLDGWMKKVPFIGAAIAR
                     NDRLKEGVALQSLRGNYQVIQGNREIFVVTGVAMNQNPVVIREVRIGGETFGSDEKSI
                     EQQEMWIGNAISAKIIRGMTAQDISDLQRLKPLKTFEIPPGDSVPFTIVFLKPAKAVK
                     KFTATVMTAEEET"
     gene            complement(10275..10475)
                     /locus_tag="VF182_02720"
     CDS             complement(10275..10475)
                     /locus_tag="VF182_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX6436017.1"
                     /translation="MFGLGLGELVIVLLILLILFNRRLPDLGDSLGKGVRKFRKSLKE
                     ADEIDITPDDATSKDRPAKKSS"
     gene            10571..11386
                     /locus_tag="VF182_02725"
     CDS             10571..11386
                     /locus_tag="VF182_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX6436018.1"
                     /translation="MEHASRSLATFFAASLALHLAVLLLWSQTSTRPVPQENVIVSLL
                     PAEEQPKAPAPSQSSQVPPQAPTRNFRSPAIIAKKNSPAVKRSAPEPARAPRKEAELK
                     KQQEYREPDSEPVPAKEPPRPPEKVEENLSITERRLPTMRELLPPANWSSSANNGDEA
                     IALNTRDPQYITYFTSIKRNIEFVWEYPEPALRYGLQGKLLLQFTILASGELESAQII
                     RSSGSNLLDHEALRAMKAAAPFRPIPPWIDKKKITITASFEYLDNRLNYRVTP"
     gene            complement(11435..>11731)
                     /locus_tag="VF182_02730"
     CDS             complement(11435..>11731)
                     /locus_tag="VF182_02730"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020154082.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX6436019.1"
                     /translation="VSTLDIAKRLLDYGFHPPTIYFPLVVSGALMIEPTETETPETLD
                     QFIEAMIAIDRESRETPDLVKNAPHTTPVRRLDEAKAARKPVLRWERERPEEEA"
BASE COUNT         2804 a         3208 c         3066 g         2653 t
ORIGIN      
        1 gtcataataa tgccgctgta gccggggtga gcggtgacca tcttaccggc ccatttggga
       61 tttaaaagat ccttccaggt ttgcggcgca tctttttccg caacgatttt cggattatga
      121 gcgatgactg aaagcgtcgc cctcatgcca tagtaaaagc cggtcttatc cttgaagccg
      181 tcgggaaaac cggcaacgct ctggggagtg aacttcagca gcaatccttt gtctttcaac
      241 agctcaaagt gaccggcgtc cgaagtatgc acgatatcgg cgttcttcaa tcccgccgtc
      301 gactcctgca tgaatctttg caaaacccgc tgcgagccgt tacggtgcac ttccacttcg
      361 atgcctttgt atttaccttg aaagtaatgc gagattgcgg tcgaagaggg caacgctaaa
      421 gacgtatgcc aaacgacctt cccttctttt cgcgccgcct cgacaagctg aggatcttgc
      481 gcgcgagctc gggaaattga aacgaaacat atgacaagaa tgaacaagca gaattttagt
      541 ttcatgatcc gcgccctcct tactgatctc gattcatggg gtgagcctac tcctattcaa
      601 ggtttcaaca actcggtcag tgatgcgatt ttcggcagct cttccagacc gttgaccgcc
      661 gccatgatct gatccaccct tgccggcggt aatcgcagct gcgcattcgc ccggaacttg
      721 ctttcgatct cctccggcgg cagtggatct tccaagccgc cccgcgggtg gggctgattc
      781 tcttccaaga tgcggccgtc gaggagacgc atcttcacgt gccccgaaaa atggcgcggg
      841 tagtcgatgc tcggatcgag ctcatagcgg actttctttg ccagggccag cacggaggga
      901 tcccggatgg cattatcggt aaattcttga agcccggctc ggccgcgaat gagcatgacc
      961 gcaatacaat aaggcaagct gaattttgcg ccataggagc ttgccggtcg ctgtttgtcg
     1021 gcaagcggct cccacaatcg aggcacggga ccctgcgccg tgcggcagac aacttcaaca
     1081 atctgttccg gcgcggctgc atacttctgc ttcagcttga gcgcgcaatc catgtagggg
     1141 tgggatatgg aaccgcacgg ataggacttg aacgtgagcc gcggcgtttc ccaaatcttc
     1201 cccaattcga gcaacttctc gaaacggtat tcggcgggcc cgccaaaaga ctgaaaaaac
     1261 ccatgggtac cctcgaaaac cttgcccgga ccgcgaaacc cttcacgagc gagcagagtg
     1321 gcgatcactc cgccgtgggc cgcccaaccg ggatgcattc gttttgtcca ggtgccgtca
     1381 gctagatatt cgatgatacc cgagctctgg ctgccacaga gtccaaacgc atcggtccat
     1441 tgacttagct caagaccgta caatttcccc gccgctgccg cagcgccaaa cgtggaacac
     1501 aatgcagtgg gatgaaaccc gcgcgcgtgg aattttcccg gagcgacaat gccgattttg
     1561 cacatgactt cggtcccggc aaccgcagct tccaaaaccg atcggccatc cgcgccaacc
     1621 tcctcgccca ccgccagagc tgtcatgccg gcgcagcaac cggtgtgcac gatggcttct
     1681 tccagtgtgt catcgtaatc gaggccgtgc gccaacgtcc cgttggcgag caccgcgttg
     1741 gccgcagcaa ccttgttggc gcaaccgatt aatcggctgt tcggcgcccc cccgagagct
     1801 tgcgccactc ttgtaaccat accgccgaaa tccattgtcg acgaggccag ggccacacca
     1861 agtgtatcga gatagaccag cttggctttt ttttgaatgc ttggaggaat atcgccgatg
     1921 ctcaagccgt ggacgaatcg tcctatagct tgcgagtacg gttccacttg tgctccttac
     1981 tgcccttaag gctcgcttgc ataaagcgtc gtgggatttt ctttcgggcc tttgagctcc
     2041 ttagcgcgcc aatcagtgaa ttcaagcttt taagctcttc cagccgcgcc acgagagcga
     2101 tgacatgtgc ggcattttcc gccggcagcg caaggctcgc acattctttg aacttttcgg
     2161 ccaactcagt ccagctcatg ggtttttgcg gatgaccacg cgcgacatca tagcgccctt
     2221 cgatcgtccg gccgtcatgg agcttaatgc gaatgcgcga gcgcacctga tcgaaaccca
     2281 aaccttccag ctcatcatcg acgtaaagcc tgacccgttt catcagttca acgatttttt
     2341 tctgccgtac tttaccatca acgaattggg caattccggc ttttcgctcc aggacggcaa
     2401 tcgccatgca gaaaggaatg ctgaactttg cttccagcgc cgtcttcggc atcggataga
     2461 ttaaggcgtt cggtacattg gagttcgtgc cgacctcaag agattcaata tcagacgcgt
     2521 gcagatcgtg ctctttgacc agatcgagaa tcaagtcctg ggcgggatga gataaggatc
     2581 cagaggggta cggtttgatg gagattccgg gctcttgcat aaaataggga cggccaaacc
     2641 ggccggcgat tttgctctcg tcgtagccgc cgcccatggc gcgatagaac cctcgctgcg
     2701 cctcgagaat gttgctggcg gctgtaaaac cgctccgggc caacaaagcc gctgttacac
     2761 cgttctcggc cgcacgcccg gcatggagcg gtttagtcat ggtgccaaag ttctcgcgca
     2821 atcccgacgc catgctggca cagataccga gggcttgaat tagaatattt tccttcaacc
     2881 gcaaaatttt ccccaccgcc atgacggccc caagtccgcc gatggtcgcg gtggaatgaa
     2941 accctgattg ataatggcgc ggattgatcg agtcggcgat gcggcactcg atttcgactc
     3001 caaggatgta tgccagcagg aactccgcgc cggagatctt ttgcttctcg ccgacggcca
     3061 aaatagccgc cagaaccggc gtcgtcggat gcgtcagcaa cccatacacc gcctctttgg
     3121 acgtggggag ctgcgtatca tcgtagtcca ttgcatgacc ggcaacacca ttggctaacg
     3181 cagctttggc ggccggcgcc gagaaggcgg ttccgagcac cgcgcactct tccctgccgc
     3241 cgaactcccg gacatgcgct tgcacgatgc gcgagcattc atccgtggac cctgccagag
     3301 ccacaccgag tccgtcgaga atgaagcccc gcgccaacgg gattacttcc gtaggcatat
     3361 ctttgtagct cgccttaaaa acgtaattcg ctacttcttg tgtcagaccc accgaagctc
     3421 cttgtctcca tccaattcat tcaataaatg gacaagactg atatcagaac tgaaaggctt
     3481 tttcaccttc tttttcaaag tgcaaccgtg acacagaggc ccctccgatt tcgtcagccg
     3541 ctaaccgttt ctctgattaa ttgggcgaga gggcccggac gtttctgttt tgctggttcg
     3601 gcattctgag gctccgcctg gttacttgac actccgaatc gtctacgata ggttcacggc
     3661 taaatattct gaaccttatt cagagtggtg gagggatcgg cccttcgaag ccacagcaac
     3721 cggttcccaa aaggaacgct ggtgctaatt ccaacccccg cgacgcgggg gaaagataag
     3781 gtgaggtgat ttaaaacctc ttcttatcga cagaagaggt ttttttttgg ccagaagtta
     3841 tgagcctcag cctgacaaca gaacagattg aacgctacag ccgtcaaata cttgtgccgg
     3901 acttcggcgg caaaggacag atacgcctgc gccagggtcg agttctgatc atcggcgccg
     3961 gaggcttggg ctcgcccagc gcgctgtact tagccgcggc cggggtgggt acgctagggc
     4021 tcatcgatgc cgatgacgtc gaactatcga acctgcagcg tcagattctc catgccaccg
     4081 ccgatctggg caaggcaaag gtagaatccg gcaaagagac acttacgcag ctcaatcccg
     4141 acgtcgacgt gattgcgtat cctgcaagac ttggttccga caatatagac ggactttttt
     4201 tacaatatca gtttattatc gacggcagtg ataattttga gactaaattc ctggtcaacg
     4261 atgctgcggt gcgcttgaga aaagcttatt cacatgccgg gatcgtcaga tggcagggcc
     4321 aaaccatgac ggtgatttcc ggcagaactg cttgttaccg ctgcctcttc aaagagccgc
     4381 cgccacccgc agaaatcttg aattgccagc aatccggcat tgtcggcgcc gttgccggca
     4441 ccattggttc gattcaagcc acggaggcca tcaagtatct tctcggcttc gaagaagggc
     4501 tgttgacgga tcgcctgctt acttacgacg ccagagccat gaagtttcat caggtacagg
     4561 tcacaaaaga cccgggttgc cacgcatgca gcgggatgta atcgaccatt cgccgattta
     4621 gaaatgattc agagtgatga ccagaaagga aggttcatga gttttgtcaa aggcctccaa
     4681 tgtagagagt gtggtcaaga ttaccccaag gaaccgctgc acgtctgcga gacctgcttt
     4741 gggccgttgg agatcgtcta cgactatgac gcaatcggca agaacatcag ccgcgaaaaa
     4801 atcgctgccc gcgaaaaaaa tctttggcgc taccgcgaac tgctgccgat cgacggagag
     4861 ccgcaggttg ggatgtactc gggtttcacg ccgttggtca aagcccatcg attggcagaa
     4921 gccttaggag tcaaagagct ttacatcaaa gatgattcgg tcaatcaccc gaccttttcg
     4981 tataaggatc gtgtcgtctc cgtggctatc tccaaggcta tcgaattcgg cttcgaaacc
     5041 gtctcgtgcg cttcgaccgg taatcttgcc aattcggttg cggcccacgc ggccaaagcg
     5101 gggctcaatt gctatgtttt tgttcccgat ggtctggagc agggaaagat tatcggctcg
     5161 gcaatttacg gtccgaagac cattgccatc gaaggaaact acgatgacgt caatcgcctt
     5221 tgcagcgaaa tcggcgacaa gtacggctgg gcgttcgtca atgtaaatct acgtccttat
     5281 tattcggaag gcgcaaaaac acacgcttat gaggttgccg aacaactggg ttggaagctc
     5341 cctaaacaca tcgtcgttgc ctccgcgggc ggaaccattc tgcccaagct agccaaagca
     5401 tttaaagaac tcgtcaaagt gggtttagtg caagatactg gttgcaaaat ttactcggcg
     5461 caggccgctg gatgcgctcc gatcatcaaa gcgcttcata acggcacaga tttgataagc
     5521 ccggtgaagc ccaatacgat cgccagttcc attgcgattg gcaatccggc tgacggctat
     5581 tatgtggttc acgcggtcaa agactccggc gggtggggtg acagtgtcac ggacgacgaa
     5641 atcgttgaag ggatcaaact gctcgcaagg actgaaggaa tctttaccga gccggcggga
     5701 ggaacggaag tcgcggtaac caggaagctc atccaaaaag gacgaattcc gcgggatgaa
     5761 tcgattgtca ttagcatcac cggtaatggt tacaaaactc tcgagtcagt atcgcgcgcc
     5821 gtcgagcagc cctacacgat caaagccaag ttggaaaatt tcgatcagct ctatcaacat
     5881 ctaaatccgt cgttttcaaa agcggcagtt agctaaattc attgccgccg cgtggcttgc
     5941 atggttccgg gccacggact atgttagcct aggcttggac gccggaatgc tttcaggagg
     6001 atggttatgt cggttcgagt acgtgttcct accccgctgc gcaaattcac tcaaggggtt
     6061 gatgaggtca acgcgcaagg aaataatatt cggtccattg tcgaagacct agaaaaaaac
     6121 tatccgggaa tcaaagaacg tatctgcgac gagaccggca aagtacgccg attcgtaaac
     6181 gtgtatgtca atggtgacga cattcgtttt ttgcaaaacc tggagacggc tcttaaagag
     6241 ggcgacaaca tctctatcgt tcccgccatt gcgggagggc gttaaagaat acgcttcgag
     6301 agctggtgaa agccaatcac aatttgcggc tgcttcacgc gctttcgcgg gagcccaaag
     6361 agatcgggtc ggtcctcgat cttatcggca acacgccgct gctggaaatc cgcaagatca
     6421 ccgaaggctt gcctgctgga gttcgcatat ttgccaagtt ggaaggattt aatcccggcg
     6481 gctcggtgaa agatcgcccg gccttgcgaa tgattcaaga aggaattcgc tcgggaatcc
     6541 taacgggcga caaggtcatc ctcgattcaa cctccggcaa caccggcatc ggcttggcgt
     6601 tgatcggtac ggtgttggga tatcgcgtcg agctggtgat tccggccaac gtcagtatcg
     6661 agcgaaagca gattattcag gcttacggcg cgaaaataac cttcagcgat ccgctggaag
     6721 gctccgatgg cgcgatccga ctaagccgtg aaatcttgcg cgccaatccc gatcgctact
     6781 tcaaaccgga tcaatatttt aatcccatga atcctcaggc ccactacgaa accactggac
     6841 cggaagtcta tcgccaaacc gatggcaaga tcactcactt cgtcgcaggt atcggcaccg
     6901 gcggaaccat aatgggcacg ggtcgctatc ttaaacagat gaacgcagac atacaggtta
     6961 tcgccgtcga acccgacgat gccttacacg gtctcgaggg gcttaagcat atggccagct
     7021 caatcgtgcc cggcatctat catgaagagc agctggacca taagattccg gtcgccaccg
     7081 aggatgccta tgcgatggta taccggttga gtcaagaaga agggatttta gtcgggcaat
     7141 catcgggcgc ggcaatgttt gccagcctga aagtagcgcg caagctgcat tccggtatca
     7201 tcgtcacggt gtttcctgat ttcggcgata agtacctatc caccagcctc tgggtgggtt
     7261 ggcgtgatcg aatggcagtt ggcaatctga agttctcaat ctaaccaatg aagaagaagc
     7321 gagaggcagt ccgccaaaaa atctacctca cttttccgag gactctcgtg cgagaaccgg
     7381 tattgtcttc aatggccaag caatttgacg tggtctttaa tattcgcggc tcgactgtaa
     7441 ccgccgaaat gggattaatg gcgctggaaa taaacggcgc gcaagccgaa gtaaacaagg
     7501 ccgttgcctg gctcaaggaa aagggagtga cggtcgattc gattgaaaaa aatgtcgtcg
     7561 agtagttatt gcgcagaggc ggaaattgaa atcaccaagg ctccgccctc atgcggcggc
     7621 ccgcctaaca gaaaataacc acggccttgg agccgcaccg tcgtgtccaa caaaggctta
     7681 gtgccctcgg tcaaacgaac cttcaaagcg atctgcttat cttttgagtc ttgaggcgag
     7741 acggcaagcg acctaccacc gggaatttcg aaggtggcat tatcgttcca tggaaccgac
     7801 tggctgtctt ccttcagcag gcgataagaa cgatacttca tcaccttcaa ctgcctttcc
     7861 attggcttga ggcgcgcatc gaattcatcg ctctcgtttg acgctagaat ggtgccgatt
     7921 tttacctgaa ccggctttga accattctct gcgccctcgg cggacaccgc cagcaacaac
     7981 aggctagaaa gaaggcttgc tttcagaagg ttccgtagac gagtcattct cgctttcttg
     8041 aggttggtac agccaaatca ccgtcgtgtt ggtttcaccc tcgtgtagca gggcgacatt
     8101 gcgcccatag gcatcaatcg attcaaccgt agccaagtta tcaggctgca tgccaagttt
     8161 tgcattgggc aagaacgaat caatggacaa tcctgcgagc acaatgacaa tcacggcagg
     8221 gaccgcccaa gcgagcctag gggcggtgaa gatgcctcga atccaatcgc cgagttcgtc
     8281 ataccatttg ctctgctgcg caatgcgttg ctgcacgcct tgccaaaacg gatcgaattg
     8341 aaccttcgag gcgctcgagc tcaacaccgt ttgcatagtg gaatgaagac gttccaattt
     8401 gtgccgctct tcccgacaag cggcgcatgc gtcaacgtgg agccggcacg cctcggcttg
     8461 cgccgggaca agttcgccat cgagccaggc tccgaggatt tctcgggtag tttcacagtt
     8521 actcataaaa ggcgccaagt ttattttgaa gttttttgcg ggcatagtgc aggcgactca
     8581 tcaccgttcc ttcggaacag ccgagaatct ccccgatgtc tttgtatgac atgccttcca
     8641 gcgtcctaag gacgatgacg gctttgtgtt cgggcgtcaa gtcatctatt gccgacagca
     8701 tcttctctct cagctcgcga tcgtgaacat ctgaaaacgg gtctttggca acttcgttct
     8761 gctgttcaaa tacctcatcc atactctcgc gaaactccag aggactcctc ttttgccgcc
     8821 gctggtgatc aatggacatg tttaccgcta tccggtacag ccaggtgtaa aaagaggatt
     8881 gcccgtgaaa gctggtgatc ttgcgataag cgcgaaaaaa cgactcttgc gcgacctcca
     8941 tggcatcgtc ccggtttcgt agtaagccaa agatgaccat gtagaccttc tgatgatatc
     9001 gcgaaaccag ttcttgaaag cttgcaatat ctccagcttg acacttctgg accagctgcc
     9061 agtcacttag ctccacattg accctgcctg tttgacgttt tccacaacct tttattcagc
     9121 cgaaacaaaa cgtgatatca aaaccttgaa gttttcgcga attggcctag aatacccctg
     9181 atatctctcc attccttcga tctacttttc acttataaag gaagcattga tgctggttaa
     9241 atgtccgagc tgcgcaacca cctacaaggt ggctgacgaa attttgacgg gcaccgctcc
     9301 cgcctttcgt tgttctcgct gcaaacacat gttcgaaggt gacctggacg cgtctgttga
     9361 ggcggtgccg ctgcgcgccg agagcgaatc aaaaattcag ggtgaagatg aaggcgagtt
     9421 ggctttcgta tttgaagcag aagatcagga cccaagggac gatcgcgacc aagacggaat
     9481 caatcctccc ccaccaaccc attttccgcc gccggatccg gtcgattcgc tggtggaaca
     9541 ggaaacgcaa cggtggtccg taccggccga aattaaagca gaagaacctt tcacaatttc
     9601 tggcgatccg agatcgcagc agattgaagg gaaaccggat cgccatgaag atggtaagcg
     9661 agcacgaaaa cctgccgaag aggttttgcc aacgatgccg accagcaaca tctcgagtct
     9721 cgagccgtac cgggatcagc aaacttcgac actaccttat ctttccttgt tcgctttgtt
     9781 gatcctgttc ttttcattag cgactgcgtt tcaatacgcc tatcccaaag ttttggacgg
     9841 ctggatgaaa aaggttcctt tcatcggcgc ggctatcgcc agaaatgacc gcttgaagga
     9901 gggcgtagcc ttacaatcgc tgcgcggcaa ctatcaggtg atacagggga atcgagaaat
     9961 cttcgtggtg acgggtgtag cgatgaatca gaatccggtt gtcatccgag aagtccgcat
    10021 tggcggcgaa acctttggct cggacgagaa atcaatcgaa cagcaggaaa tgtggatcgg
    10081 caatgcgatc tcggcaaaaa ttattcgggg catgaccgcc caagatatct cagacctgca
    10141 acgtttgaaa ccgcttaaaa ccttcgaaat tccgcccggc gattcagtac ctttcacgat
    10201 tgtttttcta aagccggcaa aagccgtcaa gaaatttact gcgacagtaa tgacagccga
    10261 agaagagacc taatctagga ggattttttt gcgggcctgt ctttgctcgt ggcgtcatcg
    10321 ggcgtaatat cgatttcgtc ggcctctttg agggatttgc gaaattttcg gacgcctttg
    10381 cccaaactat cgcccaagtc gggcaggcgg cggttaaaca ggataagcag gatgaggaga
    10441 acgatcacca actctccgag ccccagacca aacatagatc ctcctattct gttgggcatt
    10501 atttcaccaa tcgatttcat tgcaaccacg agatttgctt agacaaaggt tgccgcagta
    10561 acatacggtt gtggaacacg cttctcgatc actggcgacg ttcttcgccg cgtctttagc
    10621 tctccatctg gcggtcctgc tgctctggtc acaaacctcc acgcgccctg ttccgcagga
    10681 aaatgtcatc gtgtcgctgc tgccggctga ggagcagccc aaagcgccgg cgccgagcca
    10741 atcaagccag gtaccgccgc aggcgccgac ccgaaacttc cggtctcccg cgatcatcgc
    10801 gaaaaaaaat tcacctgcag tgaaaagatc ggcccctgaa ccggcgcgag ctccgagaaa
    10861 ggaagcggag ttaaaaaagc agcaagaata cagagaaccg gattctgagc cggtccctgc
    10921 gaaggaaccg cccaggccgc cggaaaaggt tgaagaaaat ctgtcgataa ccgagcgcag
    10981 actacccacc atgcgggagt tgttgccgcc tgcgaattgg tcttcgtccg ctaataacgg
    11041 cgatgaggca atcgcgctga acactcgaga cccccagtac atcacttatt ttaccagcat
    11101 caaacgcaat atcgagtttg tgtgggaata cccggagccc gccctcaggt acggattaca
    11161 aggaaagcta ctgctgcaat ttaccatttt ggctagtggg gagttggaaa gcgcgcaaat
    11221 cattcgttcc tcagggtcga atttgctcga tcacgaggcc ttgcgcgcaa tgaaagcggc
    11281 ggcaccattc agaccgattc cgccctggat cgacaagaaa aaaattacga tcactgcaag
    11341 cttcgagtac ctcgacaacc gcctcaacta tcgagtgacg ccttgattca cacccgcaag
    11401 ccgctcgtcg gacgagacgt tcagaatgta gagattaggc ttcttcttcc ggccgctccc
    11461 gctcccagcg aagcaccggc tttctggcgg ctttagcttc gtcgagtcga cggaccggag
    11521 tggtatgggg agcattcttt actagatcgg gagtttcgcg agattcccgg tcgatcgcga
    11581 tcattgcttc gatgaactga tcgagcgttt cgggcgtttc ggtttcagtt ggttcgatca
    11641 tcaacgctcc cgaaactacc aggggaaaat agatggtcgg cgggtgaaat ccataatcca
    11701 gcaagcgctt ggcgatatcc agagtcgaga c
//
LOCUS       DASUVS010000046         3617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_16081, whole genome shotgun sequence.
ACCESSION   DASUVS010000046 DASUVS010000000
VERSION     DASUVS010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16081"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VF182_02735"
     CDS             <1..325
                     /locus_tag="VF182_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013749105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436020.1"
                     /translation="QAKNAILAVLGADLYMKRVVIVDHDVDIFNDRQVNWAIATRCQP
                     DRDITIITNSRGSDLDPSTKEDGNTAKWGVDATAKPSLAAFTPRHRIPRDVWHRIDMK
                     DILGQ"
     gene            complement(475..1818)
                     /gene="ltrA"
                     /locus_tag="VF182_02740"
     CDS             complement(475..1818)
                     /gene="ltrA"
                     /locus_tag="VF182_02740"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEX6436021.1"
                     /translation="MSDKTGQSESQAPAVPGRARQGAETADRDRSWVKATVWSERMLA
                     ALDNGVKGGKWFSLIDKVYRKETLRAGWHKVKENAGAAGVDGQSVKQFAARAEIYLEE
                     LEQALKSGEYRPQPIRRVEIPKGGGRMRPLGIPAVKDRIVQTAVKLVIEPIFEREFED
                     SSYGFRPERSAKDALRQIDGLIQEGYTHVVDADLESFFETLEHPTLMRRIEERISDGR
                     VLELIGGFLSQDIIQGMKRWTPVGGTPQGAVISPLLANIYLHPLDRQMKQQGYRMVRY
                     ADDFVVLCRSQEQAQAALSAVRAWTEQNGLQLNADKTHVGDCRQAGQGFEFLGYRFEV
                     GRRWVRKKSLLAMRERIRQKTRRTRGDSLACIIADLNPVLRGWFHYFKHAQPWVHQLT
                     DSFVRRRLRTILRKQDKRPGMGKCRDDHLRWPNTFFAAQGLFTLTTARALARQSR"
     gene            complement(2280..3437)
                     /locus_tag="VF182_02745"
     CDS             complement(2280..3437)
                     /locus_tag="VF182_02745"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6436022.1"
                     /translation="MWNGMKVIDADAHMHEPEYLWERYVEPKFRDRVPKVAFMDGVFM
                     VYEPDGKIIPKGDLQLRPPPSAWAVLEEKYGEAYRQWWSSEIRLKDMDRYGWDIQVLL
                     PTGNNGNFAYRVALKDVELGAAMCRAYNNWCHDYCSADPKRLKFVAVLPGSDVGEMVK
                     EARRAVTELGAVSVRNPFLPEGRYLHEAEYDALWQLASELDFPISAHGEYRQRRFHPF
                     RQLEGERRGDTELALRGLDHALGFPCDNMTTLGHFIFTGILERFPKLRLAILESNAGW
                     LPFWLGRMDDHTHGRNSVMGKSEKLRMLPSEYFMRQCVISCDSDERALKYVVQDLNGE
                     NIVWNTDYPHPDAIDPKRALPDLDAQPISNEAKRKILWDNAIKLFGPRILN"
BASE COUNT          706 a         1175 c          933 g          803 t
ORIGIN      
        1 gcaagcgaag aatgcaatcc tggcggtgct cggcgctgat ctgtacatga agcgcgttgt
       61 catcgtcgat cacgacgttg acattttcaa cgatcggcag gtcaactggg caatcgcgac
      121 acgctgccaa ccggaccggg atatcaccat catcacgaat agccgcggct ccgacctcga
      181 tccatccacg aaagaagacg gcaacaccgc aaaatggggc gtcgacgcca ccgccaaacc
      241 gtccctcgcc gcgttcacgc cgagacaccg catcccgcgc gacgtatggc accggatcga
      301 catgaaagac atcctcggcc aataaactgt gcaaccgaaa tacaatagtc caacataaac
      361 gttgtcgctt gactcggatt cgcctgatag gggtcgggaa cggctgtcgc cgtccccgcc
      421 ctccgaaccg tgcgtgcggt tctcccgcac acggctctcc agtcggtagt ttcctcatcg
      481 ggattggcgc gctaacgcac gggccgtagt taaggtgaac agtccttgcg ctgcgaagaa
      541 agtattgggc caccgaagat ggtcatcacg acattttccc atccccggtc gtttgtcttg
      601 cttgcgcaag atcgtacgta gcctgcgacg aacaaaacta tcggtcaact gatgtaccca
      661 gggttgcgca tgcttgaagt agtgaaacca gccacgaagc acggggttga gatccgcgat
      721 gatgcaggcc aagctgtccc cacgcgtgcg ccgtgttttc tggcgtatcc gctcgcgcat
      781 cgccaaaaga cttttctttc tgacccaacg ccgcccgact tcgaagcggt agcccaaaaa
      841 ctcaaaacct tgcccagctt gccggcaatc tcccacgtgg gtcttgtcgg cattgagctg
      901 aagcccgttt tgttccgtcc aggctctcac tgcgctcagc gccgcttgag cttgttcttg
      961 actgcggcac agcaccacaa aatcatcggc gtagcgaacc atccgataac cctgctgttt
     1021 catctgtcgg tccagcggat gaagatagat gttggccaaa agcgggctga tcaccgctcc
     1081 ttgcggcgtt cctcccacgg gcgtccagcg tttcatccct tggataatat cctgactcag
     1141 aaacccaccg atcagctcaa gcactcgccc gtcgctgatc cgctcttcga tccggcgcat
     1201 cagagttgga tgctccaggg tctcgaagaa gctctccaag tcggcgtcca ccacatgggt
     1261 gtagccttcc tggatcagtc catcgatctg gcgaagcgcg tctttggcgc tgcgctcggg
     1321 acgaaaaccg taactggagt cctcaaactc tcgctcgaag atcggttcga tcaccagctt
     1381 cactgccgtc tgcacgatgc gatctttgac cgcaggaatt cccaatgggc gcatcctgcc
     1441 gccccctttg ggtatctcca cgcgccgaat cggctgtggc cggtattctc cgctcttaag
     1501 cgcttgctcc agttcttcca gatagatttc cgctcgggcc gcaaactgct tcacactctg
     1561 tccatccact cctgccgctc cggcgttctc tttaactttg tgccaccctg ccctaagcgt
     1621 ctctttgcgg tacaccttgt cgatcaggct aaaccatttc cctcctttga cgccgttgtc
     1681 cagcgccgcc aacatgcgct cgctccatac cgtggctttc acccacgacc ggtctcggtc
     1741 ggcggtttct gcgccttgtc tagctcttcc gggcactgcc ggcgcttggc tttcgctttg
     1801 ccccgttttg tcgctcacgc ttctaccttc ctgcctcccg tccctcggcg ctgttttgct
     1861 tccagcccct ctcgcgggtt ttcttcccgc tgcggtacca tgaaggctct gactcctgca
     1921 ccgctcacct gccgtgcagg tctccccgct tatctcgcca caccttcctg tcgttccgtc
     1981 tccaaccacg tggactgcct ggacatcgct taccaccacg ccagcgtgcc cagcgagttt
     2041 cggacttcgc cctggaatcg caggctcgtc gcagctcccc gccgaatcga gttcgttacc
     2101 ctacggaccg acactttgcc tcgggttgct ctccacctcg cttcacagcg acgcagttac
     2161 ctttggctac ggagtttgtg gcatactccg acacggactt tcaccgtgct gatgtggcgc
     2221 cctcacgggc gcacgattcc cgctaaaaac ctgcgggaat gacggactag aaatacaccc
     2281 tagttcaaaa ttcgcggtcc gaaaagcttg atcgcattat cccacagaat ttttcgcttc
     2341 gcctcgttcg atatcggctg ggcatcgaga tccggcagcg cccgtttcgg gtcgatcgcg
     2401 tcgggatgcg gataatccgt attccacacg atattctccc cgtttaaatc ttgcaccacg
     2461 tatttgagcg cgcgctcgtc gctgtcgcag gaaatcacgc actggcgcat gaagtactcg
     2521 ctcggcagca tgcgcagttt ttccgatttt cccataacgc tattgcgccc atgcgtatga
     2581 tcgtccatcc gcccgagcca aaacggcagc cagccggcat tggattcgag aatcgcgagg
     2641 cgcagcttgg gaaagcgctc gagaatgccg gtgaagataa aatgccccaa ggtggtcata
     2701 ttgtcgcaag gaaagccgag cgcgtgatcc agcccgcgca gcgcgagctc cgtatcaccg
     2761 cggcgctcgc cttcgagttg gcggaaggga tgaaagcggc gctgacgata ttcgccgtgc
     2821 gccgagatcg gaaaatccag ctctgaggcg agctgccaaa gcgcgtcata ttctgcctcg
     2881 tgcaaatagc ggccctcggg aagaaacgga tttcgcaccg agactgcgcc gagctcggtg
     2941 actgcgcggc gcgcttcttt caccatttca ccgacgtccg agccgggcag caccgcaacg
     3001 aatttgagcc gcttcgggtc cgcgctgcag taatcgtggc accaattatt gtaggcccgg
     3061 cacatcgccg ccccgagttc aacgtctttc aaagcgaccc gataggcgaa gttcccattg
     3121 ttccccgtgg gaagcagaac ttgaatgtcc cagccgtagc gatccatgtc cttgagccga
     3181 atctccgacg accaccactg ccggtaggct tcgccgtatt tttcttccaa taccgcccag
     3241 gcgctcggcg gtgggcgcag ctgcagatcg cccttgggaa tgattttgcc gtccggctca
     3301 tagaccataa agacgccgtc catgaaggcg accttcggca cgcgatcacg aaatttcggc
     3361 tcgacatacc gttcccataa atattccggc tcgtgcatat gcgcgtcggc atcgatcact
     3421 ttcattccgt tccacatgag cggctctcct ccagaacgag tcagaaatcg acgttagaag
     3481 attccggatc gaggtgtcaa gcggcagtcg ttttcaggtg actcgtcgag caatcgcgat
     3541 ctcgtgtgct ccgagcgaca gccgtaccgc gcggagctgg gatgcgttga agcgcgattt
     3601 caacatcgtc taactcc
//
LOCUS       DASUVS010000047         4386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_890264, whole genome shotgun sequence.
ACCESSION   DASUVS010000047 DASUVS010000000
VERSION     DASUVS010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890264"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /gene="groL"
                     /locus_tag="VF182_02750"
     CDS             <1..1360
                     /gene="groL"
                     /locus_tag="VF182_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941080.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX6436023.1"
                     /translation="TTTATVLARAIFSEGLKLVAAGHDPMSIKRGIDKAVAKVVEELK
                     SISKPTRDRGEIAQVGTISANNDKTIGDILAESMDKVGKEGVITVEEAKGLETTLDLV
                     EGMRFDRGYLSPYFVTDPERMECVYEDAYILIHEKKISSMKDLLPVLESVAKTGRPLV
                     IIAEEVEGEALATLVVNKIRGILKVAAVKAPGFGDRRKAMLEDIAILSDGKMIAEELG
                     IKLENVTIKDLGRAKRVIIDKDNTTVVEGAGKKPAIEGRISQIRAQIEETTSDYDREK
                     LQERLAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGIVPGGGVALIRS
                     SAGLGNLAVSEDERVGVRIIQKALEEPIRWIAQNAGLDGAVVLDKVKNSKGAFGFNAA
                     TENYEDLVKSGIVDPTKVVRTALQNAASVAGLLITTEAMIADKPEKKKDAPAMPHDHD
                     DY"
     gene            complement(1513..2481)
                     /locus_tag="VF182_02755"
     CDS             complement(1513..2481)
                     /locus_tag="VF182_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX6436024.1"
                     /translation="MELSDPRLFRDLLGQHDQHIKIIQKVLDVKIRVRGSGLEIEGDP
                     LQMELASQIIRQLYGLLEKGYPVYPSDVDYAIRILSGDSRAKLQDIFLDTIYISAHKR
                     TITPKSIAQKAYIDAIRRYDIVFGIGPAGTGKTYLAMAMAVAELMKNNFVRIILTRPA
                     VEAGEKLGFLPGDLAEKVNPYLRPLYDALHDMVDFDRARKLVERGTIEVAPLAFMRGR
                     TLNDSFVILDEAQNTTPEQMKMFLTRLGYGSKAVITGDATQIDLPAGKASGLKEAFRI
                     LKGVHGIRFVNFTEKDVARHHLVQEIITAYERDENQIASADLPDKR"
     gene            complement(2535..2960)
                     /locus_tag="VF182_02760"
     CDS             complement(2535..2960)
                     /locus_tag="VF182_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX6436025.1"
                     /translation="MKVKTSKFTETTQGFCDIIDITAKVTAKVRDAEIDDGLVALFVT
                     GSTASLTTIEHEPGLVQDIKELVEKLIPSERRYHHDDRWGDDNGFSHLRASLFGPSLQ
                     IPIARGRLVLGTWQQIILLDFDNRPRTREIVVQLIGGRE"
     gene            complement(2960..>4386)
                     /locus_tag="VF182_02765"
     CDS             complement(2960..>4386)
                     /locus_tag="VF182_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX6436026.1"
                     /translation="LTEDILRAENISVDQQGFENLMDTQRSRGREARTIAMDVFDQVA
                     VADTVIATEFIGYDQLEAESTVVALYTNGAGKTEAVEGDMIDLLTAVTPFYGESGGQV
                     GDRGIITSARGDLIEVIDTQHPTTQLTVHRGKVKRGRIQVGDKVHLEVDQTNRQKTRL
                     NHSATHILHAVMREQLGSHVRQAGSLVTPERLRFDFNHTGAISDEKLADIETTVNNRI
                     RQDAAVSIEETSFDEALRKGALAFFGDKYGDRVRVVKIGDFSTELCGGTHVHHSGQIG
                     IFKLHAEAGVAAGVRRVEAVTGEGALGLIRRYEDDLRQIGHMLRAGEDGAVERVRKLL
                     QQQKELEKEIERLRAQIGKNQIPEILARKEEINGIDVLITSVDGVDPKQLRELADQLK
                     ERLSNGFIFLASQGDHGVNLVASTSSDLAKRIHAGNIIKEVAPIVGGGGGGRPDFAQA
                     GGKNPARTDEALAEVRKIVSAKLS"
BASE COUNT         1009 a         1174 c         1176 g         1027 t
ORIGIN      
        1 aaccacgacg gccacggttc ttgcgcgtgc catcttcagc gagggactga agcttgttgc
       61 cgccggccac gatcccatga gcatcaagcg cggcatcgat aaagccgttg cgaaagtggt
      121 ggaggagctt aaatccattt cgaaacccac ccgcgatcga ggcgaaatcg cgcaggtcgg
      181 aaccatttct gccaacaacg acaaaacaat cggcgacatt ctggccgaat ccatggacaa
      241 agtcggcaaa gagggcgtga tcacggtgga ggaagccaag ggtttggaaa ctacacttga
      301 tctggtcgaa ggcatgcgat tcgaccgtgg ctatctttcg ccctacttcg tcactgatcc
      361 ggaacgaatg gaatgcgttt atgaagatgc ctacatcctg attcatgaaa aaaagatatc
      421 cagcatgaag gatcttttgc cggtgctgga aagcgttgcc aaaacaggcc ggcctctagt
      481 gatcattgcc gaggaagtcg aaggcgaggc gcttgcaact ctggtcgtca ataaaatccg
      541 aggtattttg aaggtggcag cggtcaaggc cccgggtttt ggcgatcgac gcaaggcgat
      601 gctcgaagac atcgcgattc tgagcgatgg taagatgatt gccgaggagt tgggtatcaa
      661 gctcgagaac gtaaccatca aggatctcgg tcgcgccaaa cgagtgatta tcgataagga
      721 caacactaca gtcgtagaag gggcaggtaa gaaaccagct atcgagggac gtatatcaca
      781 aattcgagcc cagatcgaag aaaccacatc cgactacgac cgcgaaaaac ttcaggaacg
      841 cttggccaaa ttggccggcg gcgttgcagt catcaaggtc ggtgctgcca ccgaagtgga
      901 aatgaaagaa aaaaaggcgc gtgtagaaga tgcgctgcac gcaacccgcg cagcggtcga
      961 agaagggatt gtgcccggcg gcggtgttgc tctgattcga tcctcagcgg gactcggcaa
     1021 ccttgccgtc tcggaagacg agagggtggg tgttcggatt attcaaaagg ctctcgaaga
     1081 accgatccgc tggatcgcac agaacgcggg actcgatggc gcagtggtgt tggataaagt
     1141 aaaaaacagc aagggagcgt tcggcttcaa cgccgccact gaaaattacg aggatctcgt
     1201 aaagtccggt attgttgatc cgaccaaagt tgtgcgcact gcgttgcaaa atgccgcatc
     1261 ggttgccggc ttgctgatta ctactgaagc catgatcgct gacaaaccgg agaagaagaa
     1321 agacgctccg gccatgcctc acgatcacga cgactactaa gctcgcgtac acagatagcg
     1381 acagtcaaag ggcgcccgaa ttcgggcgcc tttttttatg ccgtgtcccc agccgagtcc
     1441 ggcagtatgt cagggagaaa gaaaatgtca ggcgcaactc ccgtctggtc ccaaacggaa
     1501 ggggagccta gtttatcttt tgtccgggag atctgccgag gcaatctggt tctcgtcgcg
     1561 ctcgtaggcg gtgatgatct cttgcactag atgatgacga gcaacatcct tttcggtgaa
     1621 gttgacgaag cgaatgccat gcacaccctt gagaatacga aatgcctcct tcagtcccga
     1681 agccttcccc gcagggaggt cgatctgcgt ggcatcgccg gtgatgactg cctttgagcc
     1741 atagcccagg cgcgtgagaa acatcttcat ctgttcgggg gttgtgttct gcgcttcatc
     1801 gagaattacg aaggaatcat tcaatgttcg tccgcgcata aacgctagcg gcgcaacctc
     1861 gatggttccc cgctcgacca atttccgcgc gcgatcgaaa tctaccatgt cgtgcaacgc
     1921 atcatacagc ggtcgcagat acgggttgac tttctcagct aaatcgccgg gcagaaaacc
     1981 taacttctcg ccggcctcca ccgccggacg agtcaggatg atccgcacga agttgttttt
     2041 catcaactcg gcaacggcca tggccatggc gagataagtt ttgccggtac ctgccggtcc
     2101 gatcccaaat acgatgtcat agcgacgaat cgcatcaatg taagcctttt gcgcaatgct
     2161 ttttggagta atcgtgcgct tgtgcgctga aatatagatc gtgtcgagaa agatatcctg
     2221 tagtttagcc ctgctatcgc cgctgagaat gcgaatggca taatcgacgt cgctgggata
     2281 gaccggataa cccttttcca acaacccgta gagctgacgg ataatttgac tggcgagctc
     2341 catttgcaga gggtcgcctt cgatttcgag gcccgagccg cgcacccgaa ttttaacgtc
     2401 caggaccttc tggataattt tgatatgctg atcatgttgg cccaagagat cgcggaacaa
     2461 gcgtggatcg ctaagttcca aatatgtgtt ttcgcttgat agcgcactag cgatcaatcg
     2521 gttcttctcg tctttcactc cctacctcct atgagctgga cgacgatttc cctggttcgt
     2581 ggccggttat cgaaatcaag aagaatgatt tgctgccacg tgccgagaac taggcggccg
     2641 cgggctatgg gaatctgcag tgacggtcca aatagcgacg ctcgaagatg ggaaaatccg
     2701 ttatcgtcac cccatcgatc gtcatgatga taccgtcttt ccgacggaat caatttctcc
     2761 acgagctctt taatgtcttg aacgagtcct ggctcgtgtt cgatggtcgt cagagaagca
     2821 gtcgagccgg tgacgaaaag agcgaccagg ccgtcgtcga tctcggcgtc tcggactttc
     2881 gctgtcactt tggcggtaat atcgatgatg tcgcaaaaac cctgtgtggt ctcagtgaac
     2941 tttgaggttt ttactttcat caagacagct ttgcgctgac gatttttcta acctcagcca
     3001 acgcttcgtc cgttcgcgct ggattcttgc ccccagcttg cgcgaaatcg ggccggccgc
     3061 cgccgcctcc gccaacaatg ggcgccactt ctttgattat gtttcccgcg tggattcgct
     3121 tggcaagatc gctgctcgtg ctcgccacaa gattgactcc atggtcgcct tgactggcga
     3181 gaaaaataaa gccgttggac agcctttctt tgagttgatc ggcaagctct cgtaactgct
     3241 tcggatcgac tccgtcgacg gaggtgatta agacgtcgat gccgttgatt tcttctttcc
     3301 tggccaagat ttccggaatc tggttcttgc caatttgagc gcgcaaacgc tcaatctcct
     3361 tttctagctc cttttgctgc tgcaatagtt tcctgactcg ctcgaccgcg ccatcttcgc
     3421 cggcgcgcag catatggccg atttgcctta agtcgtcctc atagcgtcgg atcaggccga
     3481 gcgccccttc gccggtaacg gcttcgaccc gtcgcacccc ggcagcaaca cctgcctcgg
     3541 cgtgcagctt gaaaatcccg atttggccag agtggtgaac gtgagtgccg ccgcacaact
     3601 ccgttgaaaa atcgccgatc tttaccaccc gcacccgatc accgtactta tcgccaaaaa
     3661 atgctaacgc gcctttacgc agcgcctcat cgaagcttgt ctcctcgatg gaaactgccg
     3721 catcctgtcg gatacggttg ttgacagtgg tttcgatatc ggcaagtttc tcatcggata
     3781 ttgcaccggt gtggttgaaa tcgaaacgca gccgttccgg cgttaccaga gaaccggctt
     3841 gccgcacatg gctgcctagt tgctctcgca tgaccgcatg aaggatatgt gtagccgagt
     3901 gattcaagcg ggtcttttgg cgatttgttt gatcgacttc tagatgcact ttatcgccga
     3961 cttgaatgcg gcctctcttt actttgccgc gatgaactgt gagttgcgtc gtcggatgtt
     4021 gagtatcgat cacttcgatg aggtcgcctc gcgcgctggt aatgataccc cggtcgccaa
     4081 cctggccgcc ggattcaccg taaaaaggtg tcacagccgt gagtagatcg atcatatcgc
     4141 cttcgacagc ttctgttttc ccggctccgt tagtatatag cgcgacgacg gtcgactctg
     4201 cctcgagttg atcgtagccg atgaattcag ttgctattac cgtgtccgca accgcaacct
     4261 ggtcaaaaac gtccatcgca atcgtccgcg cttcacgacc ccgggaacgt tgtgtgtcca
     4321 tgagattttc gaaaccttgc tgatcgactg aaatattctc ggcgcgaaga atgtcctcag
     4381 tcaggt
//
LOCUS       DASUVS010000048        10337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_17299, whole genome shotgun sequence.
ACCESSION   DASUVS010000048 DASUVS010000000
VERSION     DASUVS010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10337
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17299"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VF182_02770"
     CDS             <1..339
                     /locus_tag="VF182_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX6436027.1"
                     /translation="EDRKRREEIEARNTADSMAYQVERQVSELGDRVPMNERARAEQL
                     ISQIRELVESNSTDVTRLRQLTSDLQQVGYGLAAAASGHDNTGGQGPGGGPSTSGGND
                     DVIDAEFKET"
     gene            381..887
                     /locus_tag="VF182_02775"
     CDS             381..887
                     /locus_tag="VF182_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX6436028.1"
                     /translation="MEAPDKSENRVSDQAEPAAESEAEIARLSEELRRERDALLRTLA
                     DFENYRRRTERDRAAAAQSGKRDVIVSLLAVLDDFDRALQHMDDAPPSVAQGMEAIQR
                     NLIAVLERNGVTRFNSVGEAFDPQWHDAVATVASGEMQPGTIAEELQSGYKWGEELLR
                     PARVRVAQ"
     gene            966..2012
                     /locus_tag="VF182_02780"
     CDS             966..2012
                     /locus_tag="VF182_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011792175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX6436029.1"
                     /translation="MAVEFRDYYQVLGVNKNAAEDEIKSAYRKQARKYHPDVNPGDKS
                     AEEKFKELNEAYEVLSDPEKRKRYDALGPNWKAGEEFRPPPGWQRASRGNFRDMEDVF
                     GGMGGLGGGGSGFSDFFDALFGGPRARRGGRGGASFSMRGRDVEAEIELTLEEAHRGV
                     KRSIALQATEICADCRGSGTKDSSACPTCHGAGMIQRPKTLDVTIPSGVREGAAIRLA
                     GQGEAGAHGGRPGDLLLRVRLRPHRLFDVIGESDLQLELPVAPWEAALGARVSVPTLD
                     GPVEMTLPAGTQAGQRLRLRGQGLNRRAGGRGDQYAKIKIVNPPRLTNTEKEFFERLK
                     SESHFDARELMPNR"
     gene            2009..2323
                     /locus_tag="VF182_02785"
     CDS             2009..2323
                     /locus_tag="VF182_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HEX6436030.1"
                     /translation="MKFIRYEIILCPPEKQPLKLDALAERAGVHPILIQRFIDCGLLE
                     PSGREGGELFFDEETVPRLQMIRRLRDHLGINVAGIAVILDLRDRLKGLQRENAVFRA
                     RL"
     gene            2595..3002
                     /locus_tag="VF182_02790"
     CDS             2595..3002
                     /locus_tag="VF182_02790"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX6436031.1"
                     /translation="MPRVSGAGTGADRIYVTARLNRLLAQAEDEAKQLKDDYISIEHL
                     LLAMMDDTGVAGRLFKEFGLTRERLAGALKSIRGNQRVTSQNPEATYEALARYGRDLS
                     GRRRIRSSIQRPAAEAGDSKRTRDFAWQALAQG"
     gene            2941..3114
                     /locus_tag="VF182_02795"
     CDS             2941..3114
                     /locus_tag="VF182_02795"
                     /inference="COORDINATES: protein motif:HMM:NF021915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436032.1"
                     /translation="MKRAIQKELETSLGKLLLKGEVSDGQTVIVDADETRGISFQVKS
                     GGESQPHIPGRAA"
     gene            3420..3890
                     /locus_tag="VF182_02800"
     CDS             3420..3890
                     /locus_tag="VF182_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX6436033.1"
                     /translation="MFGELMRYNPWEELSSWHRDIDDLFGRFFGHSEASSSLGNWVPR
                     METYRKDNEFVLRFDLPGVNPNDVQVQAEGNVLSISGERKSEENREGYQETSYGKFER
                     TVTLPQGIEADKIAARYEHGVLEIHVPLTAQLAGRKIPVQIEQKENKKVESKAA"
     gene            complement(4025..5932)
                     /gene="glgB"
                     /locus_tag="VF182_02805"
     CDS             complement(4025..5932)
                     /gene="glgB"
                     /locus_tag="VF182_02805"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX6436034.1"
                     /translation="MSNHGFSLITNDDSHLFNEGSHFRLYDKLGAHVVQHAGDSGTYF
                     AVWAPNAEQVSVIGNFNDWDKRRQPLSPKASTGIWEGFVPGIGQGSLYKYHIRSRVSG
                     YRVDKADPFSCFNEIPPRTASIVWDLDYQWNDQDWMAKRQQHNRLDKPMAIYEMHVGS
                     WRRVLEQGNRSLSYRELAAPLAEYLQHLGYTHVEFLPIMDHPFFGSWGYQITGYFAPS
                     GNYGTPQDLMYLIDYLHQHDIGVIFDWVPSHFPTDEHGLAFFDGTHLYEHADPRQGYH
                     PEWKSYIFNYGRSEVQSFLISNAMFWLDRYHVDDLRVDAVASMLYLDYGRRAGEWIPN
                     KYGGHENLEAINFIRRMNQEVYQHCPAIQTIAEESTSWPMVSRPNYLGGLGFGLKWDM
                     GWMHDTLEYMSRDPVHRRYHHNKLTFRMIYAFHENFVLPLSHDEVVYGKGSLLGKMPG
                     DRWQKFANLRALYGYMYAQPGKKMLFMGGEFGQPSEWAHDGSLPWELLSDPMHRGLKD
                     WVSDLNHLYRSDPALHELDCDPGGFEWIDCDDAEHSVISLIRKGKSTKDLILVICNFT
                     PVPRYSYRLGAPRAGIWQELLNSDSARYGGSNMGNLGGVATVPIPLHARPHSLTLTLP
                     PLSVSLFRNSG"
     gene            complement(6019..9387)
                     /gene="treS"
                     /locus_tag="VF182_02810"
     CDS             complement(6019..9387)
                     /gene="treS"
                     /locus_tag="VF182_02810"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX6436035.1"
                     /translation="MSRKPAKSTSFLLDDNPLWYQDAVIYEVHVRAFHDGNGDGIGDF
                     QGLTEKLDYLQDLGVTALWLLPFYVSPLKDDGYDIADYTEIHPVYGTLHDFLLFLRAA
                     HERGLRVITELVINHTSDQHPWFLRARHAKPGSSARDFYVWSDTQERYSEARIIFKDF
                     EPSNWSWDSVAKAYYWHRFYSHQPDLNFDNPEVHEAIFDALDFWLDIGVDGLRLDAVP
                     YLYEREGTNCENLAETHAFLKKLRQHVDSKYKNRMLLAEANQWPEDAVGYFGNGDECH
                     MAFHFPVMPRLFMSLYMEDRFPIIDILDQTPAIPESSQWALFLRNHDELTLEMVTDED
                     RDYMYKVYASDPRARINLGIRRRLAPLLANNRRRVELLNSLLFSLPGTPVIYYGDEIG
                     MGDNIYLGDRNGVRTPMQWSADRNAGFSRANPQRLYLPVIIDPEHHYETVNVDAQQQN
                     PASLLWWMKRLVLLRKNYQAFGRGSIEFLLPDNRKVLVFVRRLGDEIILVVANLSRFV
                     NYVELDLAAYKGMVPVELFGHTRFPSIGELTYFLTLGPHAFYWFKLEPTRVSEARAGV
                     EGFEPPRLEVEGRWEAVLEGRPRTMLERTLPAYLLTCRWFGGKAQQIRSVKNVDVIPF
                     PVESATIYLTTWAVDYIGGTPETYLLPLAYASGDRAFELRQSAPQALIAQLKIEDEAG
                     ESEGILYDAVHEPGFLKSLLSALARGRRYKDAGAELLAHPSRTFRKLVGSTENALEPL
                     LIKRDQSNTSVAYGDRLMFKLFRRITEGTNPDVEIGRFLTDLEFAHVPPLAGHIELRK
                     GRGEPATIGILQGMVANEGDAWRYTQDSLGYYFEEVLTRHVDVGDASLPNGSPVELAH
                     NGAPGMAKELIGTYLGSACLLGQRTGEMHVALASDSKDTAFAPEPFTALYRRSLYQSM
                     RSLADQSLRLLQKRLKRLPDETQRQAAELLTLEAEILKRSRQLVEKKIGGMRIRSHGD
                     YHLGQVLYTGKDFVIIDFEGEPARSITERRLKRSPLRDVAGMLRSFNYAVLTKLRGGA
                     LRPEDAVQLKPWGRYWEFWISVSYLNGYFDVTRQSGLLPKTRDDLNLLMNILLLDKAI
                     YELRYELDNRPDWVAVPIEGILELIKSKTTVGLQ"
     gene            complement(9433..>10337)
                     /locus_tag="VF182_02815"
     CDS             complement(9433..>10337)
                     /locus_tag="VF182_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEX6436036.1"
                     /translation="KRVLLFWCEQGVRIFRVDNPHTKAFRFWQWVIAEVKKDYPETIF
                     LAEAFTRPKVMYRLAKLGFSQSYTYFTWRNTKWELTQYFTELTRTDVVEFFRPNLWPN
                     TPDILSEYLQAGGRAAFMSRLILAATLGANYGIYGPAFELCVNAPREPGSEEYLDSEK
                     YQIKEWNRESPDSLRELIARVNHIRTANPALHRNRSLRFYTVDNDEIICFAKQTEDRS
                     NIIVVAVNLDPHHIQSGWVDLPLAELGLDSAKPFQMHDLLTDARYLWNGPRNYLSLDP
                     ASVPAHVFRVRPNLRREQDFDYFM"
BASE COUNT         2370 a         2838 c         2988 g         2141 t
ORIGIN      
        1 gaggaccgca agcgccgcga agaaatcgaa gcccgtaata cggccgattc gatggcatat
       61 caagtggagc ggcaagtgag cgagcttggt gaccgcgtgc cgatgaacga gagagcccgc
      121 gcggagcaac taatctcgca gatacgggag ttggtggaaa gcaattccac cgatgtgacg
      181 cgactgcggc aactcacgag cgatcttcag caagtcggct atggactcgc tgccgcagct
      241 tccggccatg acaataccgg cggccagggg ccgggaggcg ggccatccac ctcgggcggc
      301 aatgatgacg tgatcgatgc tgagttcaag gaaacttgat tggaaatgga gcttggtccc
      361 aatgtttctc ggggtaaatc atggaagcac ctgataaaag tgaaaaccgg gtgagcgatc
      421 aagcagagcc ggccgccgag tctgaggcgg aaatcgcaag gctcagcgaa gagttgcgac
      481 gcgagcgcga cgcgcttctg cgcaccctgg ctgactttga gaattatcgg cggcgtacag
      541 aacgggatcg cgccgctgcg gcacaaagcg gcaagcgcga tgtcattgtc tcattacttg
      601 cagtgcttga cgatttcgac cgcgccctcc agcacatgga cgatgcgccg ccatccgtag
      661 cgcaagggat ggaggcgatt cagcgcaatc tcattgccgt gcttgagcgt aatggcgtga
      721 cccgattcaa cagcgtgggc gaagccttcg atccgcagtg gcacgatgcc gtcgccacgg
      781 tggcgagcgg tgaaatgcaa ccggggacga tcgccgaaga actccagagc ggctacaagt
      841 ggggcgagga attgctcaga cccgcgcggg tgcgcgtcgc gcaatgaagc ccggcgctct
      901 tcgttgggcc tgatgggtgt ttgattattc aaagccgctt ggaatccaag ctctttgata
      961 actaaatggc agttgaattc agagactact atcaggtgct cggcgtcaac aagaacgccg
     1021 cggaagatga gatcaagtcg gcgtaccgca agcaggcgcg caagtaccac cccgacgtca
     1081 atcccgggga caaatcagca gaagaaaagt tcaaggaact taacgaagcc tacgaggtcc
     1141 tgtcggatcc ggaaaagcgc aagcgttacg acgccctcgg gcctaattgg aaagccggcg
     1201 aagagttcag accccctcct ggctggcagc gggcatcccg aggtaacttc cgcgacatgg
     1261 aggatgtgtt cggcggtatg ggcggccttg gcgggggagg cagcggattc agcgatttct
     1321 tcgatgctct tttcggtggc ccgcgcgctc gtcgcggcgg ccgcggtggc gctagttttt
     1381 cgatgcgcgg ccgcgatgtc gaagccgaaa tcgagctcac gctcgaagag gcgcatcgcg
     1441 gcgtcaagcg cagcatcgcc ctgcaagcca cggaaatctg cgccgactgc cgcggcagcg
     1501 gcacaaagga ttcttcggca tgcccgacct gtcacggcgc tggtatgatt cagcggccga
     1561 agacgctcga tgtaaccatt ccgtcaggag ttcgcgaagg agccgcgatt cgcctggccg
     1621 gacagggcga ggccggcgct cacggcggca ggccggggga tctgctgctg cgcgtgcgcc
     1681 tccgaccgca tcgtctgttc gatgtcattg gcgagagcga tttgcaactg gagctgccgg
     1741 tggcgccctg ggaagctgcc ctgggggcca gggtttcggt gccgacgctc gatgggccgg
     1801 ttgaaatgac ccttcctgcc ggaacccaag ccggtcagcg gctcaggttg cgcggccagg
     1861 gcttgaatcg cagagccggg gggcgcggtg atcaatacgc gaaaatcaaa atcgtcaacc
     1921 caccgcggct gaccaacacg gaaaaagaat tctttgagag actgaaaagc gaatcacatt
     1981 ttgacgcgcg ggagttgatg cccaaccgat gaaatttatc cggtatgaaa ttattctttg
     2041 cccgcccgag aagcagccgt tgaagctcga tgcgcttgcc gaacgcgctg gcgtccaccc
     2101 aattctgatc cagcgtttta tcgattgcgg gctgctcgag ccgagtgggc gtgaaggcgg
     2161 ggaattattc ttcgatgaag aaactgtacc gcgcctgcag atgattcgca ggctgcgcga
     2221 ccatctggga atcaatgttg ccggcattgc cgtcattctg gatttgcgcg atcgcctcaa
     2281 ggggctgcag cgtgaaaacg ccgttttccg agcaaggctg taaaacgatt ccatccgatt
     2341 cgtattcggg gtcgagcgcc agttaaaaaa cggaggccta aaacggatat caaccggcta
     2401 acacaaaagg ctcaggaagc gctcggcacc gctcagtcca acgcggcgcg tttatggtca
     2461 ccagcaggtg gagatcgagc atctgctctc ggcgctgctc gatcaagaag ccgggctgcg
     2521 acctcgatca tttccaaagc caacataacg atgtaaacgg tttcaaacgt cgcctggagc
     2581 aggagcttga acgtatgccg cgggtttccg gtgcgggcac gggcgctgac aggatctacg
     2641 tcaccgcgcg cctgaaccgc ttgttggctc aggcggagga cgaagcgaag cagctcaaag
     2701 acgactacat ttctattgag cacctactcc tggccatgat ggacgacacc ggcgtggcgg
     2761 gcaggctctt caaagaattc ggtcttacgc gcgaacgatt ggcaggcgcg cttaaatcga
     2821 ttcgcggcaa ccagcgggtc acttcgcaga atccggaagc cacttacgaa gctctagccc
     2881 gctacggtcg cgacctatct ggccgccgcc ggatacgatc ctcaatacag cgcccggccg
     2941 ctgaagcggg cgattcaaaa agaactcgag acttcgcttg gcaagctctt gctcaagggt
     3001 gaggttagcg atggccagac cgtcatcgtc gatgcagatg aaacgcgcgg gattagcttc
     3061 caagtgaaat ccggcggaga gtcgcagccg cacattccgg ggagagcagc atagctttcc
     3121 atatatccat aacgacagcc gtggtaagct cgtgctttcc tgagtccgag gagaagatcg
     3181 atctggcgcc gactctcaac tcaattcgtt ggcaagcgcc gcattgtttg ccttacatat
     3241 aacgcgatcg ccgcggcgtg cgcatttcag acataatcat atctgctctt gcagctttgt
     3301 gttgtgagtt ctaagtagct caaaaaaatc tggaattttg ccttatcgga ccttgacaga
     3361 agcccagtcg tcattaaatt tcagtcagcg attcatgata atgaaaggag gtactaagta
     3421 tgttcggtga gttgatgaga tataatccgt gggaggaatt gtcgagctgg catcgtgaca
     3481 tcgacgacct gtttgggcgg ttcttcggac attctgaagc tagttcctct ttgggcaact
     3541 gggtgcctcg gatggagaca taccgcaagg acaatgagtt cgttttacga ttcgatcttc
     3601 cgggtgtaaa ccccaacgac gttcaggtcc aggcggaagg gaatgttctg tcgatctccg
     3661 gggaacgtaa atcggaggag aacagagagg gttatcagga gacttcttac ggcaagttcg
     3721 aacggacagt gactctgccg caaggcatcg aagccgacaa gattgccgcc cgctatgaac
     3781 atggggtgct ggagatccat gtgccactta cggcccagct tgctggacgc aagatcccgg
     3841 ttcagatcga gcaaaaagag aataagaagg tggaatccaa agccgcatga tttagaaccg
     3901 gctttatcac cgccttttcc cggcgctaaa aaggccctcc acaagggggc cttttttatt
     3961 cgtcaacggg ttgttgggcg gacaatcgat cgagctactt ttaaaatcga tgtcgaatag
     4021 ctcctcatcc ggaatttcta aacaaggaaa ccgacagcgg cggcaaggta agggtaagag
     4081 agtgcgggcg cgcatgtagt ggaatcggca cggttgcgac gccgcccaaa ttgcccatgt
     4141 tgctgccgcc ataacgggcg gaatcgctgt tcaacaattc ttgccagatc cctgcccgcg
     4201 gcgctcccaa tcgatagctg taccgtggca ccggggtgaa gttacagatc accagaatca
     4261 aatccttggt cgattttccc ttgcggatga gactaatgac gctgtgctcg gcatcgtcgc
     4321 agtcgatcca ctcaaagccg ccgggatcgc agtcgagctc atgaagtgcc ggatcgctgc
     4381 gatataaatg attgagatcg ctgacccagt ctttcaatcc tcgatgcatg ggatcgctta
     4441 acaactccca cggcaagctg ccgtcgtgcg cccattcgct gggctggccg aactccccgc
     4501 ccataaacag catcttcttg cccggttgcg catacatata gccgtagagc gcgcgcaggt
     4561 tggcaaactt ctgccagcgg tcgccgggca tcttgccgag caaggatcct ttaccgtaga
     4621 ccacctcgtc atgggagagc ggtaagacaa agttctcatg gaacgcataa atcatccgaa
     4681 aggtcagctt gttatggtga tagcgccggt gcaccggatc gcggctcatg tactccaagg
     4741 tgtcgtgcat ccagcccata tcccacttga gcccaaagcc cagaccgccc agataattgg
     4801 gacgtgaaac catcggccac gaagtggact cttcggcgat ggtctggatc gccgggcaat
     4861 gctggtagac ctcctggttc atgcgcctga taaaattaat cgcttccaag ttttcatgac
     4921 cgccgtattt gttcggaatc cattcgcctg cccggcgacc ataatcgaga tagagcatgg
     4981 aggctaccgc atcgacgcgc aaatcatcga catggtaacg gtcgagccag aacatggcat
     5041 tgctgataag aaagctttgc acctcggagc ggccataatt gaagatgtag ctcttccatt
     5101 cgggatgata gccctggcgc gggtcggcat gctcgtatag atgcgtgccg tcaaagaatg
     5161 ccaagccgtg ctcgtcggtg ggaaagtgtg acggcaccca atcaaagata accccgatat
     5221 cgtgctggtg cagataatcg atcaggtaca tcaagtcctg cggtgtgcca tagttgccgg
     5281 acggggcaaa gtagccggtg atctggtatc cccacgagcc gaaaaacggg tggtccataa
     5341 tcggcagaaa ttccacatgg gtatagccca gatgttgaag gtactcggcc aacggcgcgg
     5401 ccagctctcg ataggataat gagcggttac cttgctcgag cacgcggcgc cacgaaccga
     5461 cgtgcatttc gtagatcgcc attggtttgt cgagccggtt atgttgctgg cgcttggcca
     5521 tccagtcctg atcgttccat tgatagtcga gatcccacac aatcgatgcg gttctgggcg
     5581 gaatctcgtt gaagcaagag aaagggtcgg ctttgtcgac gcggtaaccg ctcacgcgcg
     5641 agcgaatgtg atacttgtac aagctgcctt ggccgatacc cggaacgaag ccttcccaga
     5701 tgccggtgct ggctttcggc gaaagcggct ggcgtctctt atcccagtca ttgaaattgc
     5761 cgatgaccga aacctgttcc gcgttgggcg cccagacggc gaagtaagtg ccgctgtcac
     5821 cggcgtgctg caccacgtga gcgccgagtt tgtcatagag acgaaaatgg ctgccctcat
     5881 tgaatagatg gctgtcatcg ttggtgatga ggctgaagcc gtgattggac attattgctt
     5941 aactccttga cagaagcgga cggatcaacg gcagcatcgg ctcgacggca acattcacga
     6001 gatacgaaag gtagaatatt actgcaagcc aacggtcgtc ttgctcttga tcaattccaa
     6061 aattccttcg attggcaccg ccacccaatc gggacgattg tcgagctcgt agcgtaattc
     6121 atagatggcc ttgtccagca acagaatatt catcaatagg ttgagatcgt cgcgggtttt
     6181 gggaagcagc ccagattggc gagtgacatc gaaatacccg tttagatagc tcacggaaat
     6241 ccaaaactcc caatagcgac cccaaggctt gagctgaacc gcatcctcag gccgcaacgc
     6301 gccgccgcgc agcttggtca gcacggcata gttgaacgat cgcagcatac ctgccacgtc
     6361 gcgcagcggc gagcgtttca gacgccgttc ggttatcgac cgcgccggct caccttcgaa
     6421 atcgatgata acgaaatcct tgcccgtata taatacctga ccgagatggt aatccccgtg
     6481 agaacgaatt cgcataccgc cgattttttt ttcgaccagt tgccggctgc gctttaagat
     6541 ctcggcctcg agagtcaaaa gttctgcggc ctgcctctgg gtttcatccg gaagccgctt
     6601 caaacgcttc tgcaagaggc gcagagactg atcagccaaa ctgcgcatgc tctgataaag
     6661 tgagcggcga tacagagcgg taaacggctc cggcgcgaaa gccgtatcct tgctatcgga
     6721 cgcaagcgcc acatgcatct cgccggtacg ctggccgagc aggcaagccg atcccaaata
     6781 agtaccaatc aattctttgg ccatcccggg agctccgttg tgggcgagtt ctaccggcga
     6841 tccattgggc aaactcgcgt cgccaacatc gacgtggcgc gttaaaactt cttcgaagta
     6901 atatcccaag ctgtcctgag tatagcgcca ggcatcgcct tcattcgcta ccattccctg
     6961 caagatgccg atggtagcgg gttcgccgcg gcccttgcgc aactcgatat ggccggccag
     7021 tggcggtaca tgcgcaaatt ccaggtcggt aagaaaccgg ccgatctcaa cgtcgggatt
     7081 ggtcccctcg gtaatgcggc gaaacagttt gaacatcagc cgatcgccat aggctaccga
     7141 cgtgttgctc tgatcgcgtt tgatgagcaa tggttccagc gcattttccg tgcttcccac
     7201 cagtttacgg aacgttctgg acggatgcgc cagcagctcg gcgccggcat ctttgtaacg
     7261 ccgtccccgg gccaaggccg acagcaatga tttgaggaac cctggctcat gcacggcgtc
     7321 gtacaggatt ccttcgcttt cgccggcttc atcttcgatc ttcaactgag cgatgagcgc
     7381 ttgcggcgcc gattggcgca gctcgaaagc ccgatcaccg ctggcgtacg cgagaggaag
     7441 caggtaggtt tccggcgtcc cgccgatgta atccacagcc cacgtcgtca agtaaatggt
     7501 cgccgattcc accgggaatg ggatgacatc gacattcttc accgaccgga tctgctgcgc
     7561 tttgccgccg aaccagcgac aggtcagcag gtaagcggga agagtccgtt cgagcatcgt
     7621 cctcggcctg ccctccagaa cagcttccca cctaccctcc acctccaagc gtggcggctc
     7681 gaagccctca actcccgccc gcgcttccga aacccgcgtc ggttcgagct tgaaccaata
     7741 aaatgcatgc ggccccaaag tgagaaagta agttagttca ccaatcgatg gaaaacgcgt
     7801 gtggccgaag agctccaccg gaaccatgcc cttgtaagcc gccagatcga gctcgacata
     7861 attgacgaaa cgcgacaggt tggccacgac cagaatgatt tcatcgccga gccggcggac
     7921 aaacaccagc actttgcggt tatccggtag aagaaactcg atgcttcccc gtccaaaggc
     7981 ctgataattt ttacgcagca acaccagccg cttcatccac cacaggagcg atgccgggtt
     8041 ctgctgctgg gcatcgacgt tgacggtctc ataatgatgt tccggatcga tgatcacggg
     8101 cagataaagg cgctgcggat tggcgcgaga gaatccagca ttgcggtcag cgctccactg
     8161 catcggggtg cgcacgccgt tgcgatcgcc aaggtaaatg ttatcgccca tgccgatctc
     8221 atcgccgtag taaatcaccg gagtcccagg caatgagaac aacaggctat tgagcagctc
     8281 gacgcggcgg cgattattgg ctaaaagcgg cgccaggcgg cgccggattc cgagattgat
     8341 ccgcgcccgc ggatcgctgg cgtagacttt gtacatgtag tcgcgatctt catcggtcac
     8401 catctcgagg gtcagctcat cgtggtttcg caagaacaac gcccattgac tcgattcggg
     8461 tatggccggc gtctgatcga gaatgtcgat gatcggaaag cgatcctcca tatataacga
     8521 catgaacagg cgcggcatga ccggaaaatg aaacgccatg tggcattcat cgccattgcc
     8581 gaaatacccc actgcgtcct cgggccactg attggcttcg gccaacagca tgcggttttt
     8641 atatttgctg tcgacatgtt ggcgcagctt tttgagaaac gcgtgagtct cggcaagatt
     8701 ttcgcagttg gtgccctcgc gctcgtaaag ataaggcacg gcgtcgaggc gcagcccgtc
     8761 cacgccgata tcgagccaga agtcgagcgc atcgaaaatc gcctcgtgca cctcaggatt
     8821 gtcaaagttc agatccggct gatgtgaata aaaacggtgc cagtaatagg ctttggcgac
     8881 ggaatcccag gaccagttgg acggctcgaa gtctttgaaa atgatccttg cttcggaata
     8941 acgctcctgc gtatcgctcc agacgtaaaa gtcgcgcgcg ctactgccgg gcttggcatg
     9001 ccgcgcccgg agaaaccatg gatgctgatc ggaggtgtga ttgatgacca gctcggtaat
     9061 gacacgcagg ccgcgttcgt gcgccgcgcg caagaagagc agaaagtcgt gcaacgttcc
     9121 atagacggga tggatctcgg tatagtcggc gatgtcgtaa ccgtcgtctt tgagcggcga
     9181 aacatagaag ggcagcagcc acagtgctgt cacgcctaaa tcttgaaggt aatcgagctt
     9241 ttcggtcaat ccctgaaaat cgccgatgcc gtcgccgttg ccgtcgtgaa aggcgcgcac
     9301 gtgcacttcg taaataaccg cgtcctgata ccagagcggg ttgtcgtcga gaagaaacga
     9361 tgtggattta gcgggtttgc ggctcaattt tcgctttgct ctctcttgtt tgctgaaaac
     9421 cgtttgcgtc tgctacatga agtaatcgaa atcctgctcc cgccgcaggt tgggtcgcac
     9481 tcgaaagacg tgcgccggca cgctcgcagg atcgaggctc aagtaattgc gcggcccgtt
     9541 ccacaggtag cgcgcatcgg tcagcagatc gtgcatctgg aatggcttgg ccgaatccag
     9601 accgagttcc gccagcggca aatccaccca gccggattga atgtggtgcg gatcgaggtt
     9661 gacggcaacg acaataatgt tactgcgatc ttcggtttgt ttagcgaaac agataatctc
     9721 gtcattgtcc acggtataaa aacgcaaact gcgattacga tggagcgcgg gatttgccgt
     9781 gcgaatatgg ttgacccgcg cgatcagctc gcgcaggctg tcgggcgatt cgcggttcca
     9841 ttctttaatc tggtattttt cggaatccaa atattcctcg ctgcccggtt cacgcggcgc
     9901 gttgacgcag agctcgaacg ccgggccgta gatcccatag ttggcgccta aagtggcggc
     9961 aagaatcagg cgcgacatga aggccgcgcg cccgccggcc tggagatact ccgagagaat
    10021 atcgggagtg ttcggccaca ggttgggacg aaagaattcg acgacatcgg tccgagttag
    10081 ctcggtgaaa tattgggtca gctcccactt ggtgttgcgc caggtaaaat acgtgtacga
    10141 ctggctgaaa ccgagcttgg ccaggcggta catgactttg ggccgagtga acgcttcggc
    10201 aagaaatatc gtttccgggt aatctttttt gacttcggca atgacccatt gccaaaagcg
    10261 gaaggctttg gtgtgaggat tatcgacccg aaaaatccgc acaccctgtt cgcaccagaa
    10321 taatagaact ctcttca
//
LOCUS       DASUVS010000049         3573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_17368, whole genome shotgun sequence.
ACCESSION   DASUVS010000049 DASUVS010000000
VERSION     DASUVS010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17368"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VF182_02820"
     CDS             <1..1083
                     /locus_tag="VF182_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX6436037.1"
                     /translation="ITAERLRLFDFTLPVRDNVSEIVVMNKADPAPAKLDELAGLEVH
                     VRKTSSYYESLRGLNERFRRAGRPQMKLTLVPDELEDEDLMEMVAAGLVRTIVVDDWK
                     AGLWAEILPKIQLRPELALRTGAKTGWATRKNTPRLKAVLNEFITEHLKGTNRAAIHL
                     ASYQRRFKALHDSTAAPEWRKFEETIAYFEKYGQQYGFDHLMLAAQGYQESRLDQRAR
                     SRVGAIGIMQIMPATGKELNVGDIRNTEPNVHGGAKYMRRLFDQYFKNANFDEQNRAL
                     FAFASYNAGPGRIAKVRAEAKDRRLDPNKWFNNVEIVASNRIGQETVHYVRNIYKYYT
                     AYRLQLDAIAAQRMAKQQIDSKTAKN"
     gene            complement(1515..1898)
                     /locus_tag="VF182_02825"
     CDS             complement(1515..1898)
                     /locus_tag="VF182_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436038.1"
                     /translation="MHIQIKQFSWAEGNYMIIIAQGILNVRSLEQILIKVAAASLARA
                     HCKVLIDFVDASCTIVPTQIDRLFSRRWPGLYPSPSKIALVSPSDDNHHASVSDVSTC
                     LGKHGLRVAVFRDSKAAVDWLADLV"
     gene            2441..3478
                     /locus_tag="VF182_02830"
     CDS             2441..3478
                     /locus_tag="VF182_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX6436039.1"
                     /translation="MAGSKNPMISMSAAAGLLQAIEAAGANPDDVLEAIAMDRAIFSN
                     PDSFIRVSSFARILEEAARLSSDPCFGLHFGESYNPKNIGPLVYVVLNSPTIAAGIEN
                     AARYITIHNEAASASLLVENHRAYLRFLPNELLINSSRQHSEYAMTVTLNTLRLMVGS
                     QWAPQEVHFTHQLQAATPEHFRVFCAPIMFGCSANALVMESDFLKREVPAADPRLYHI
                     LKRYLGGVLKDMPREDDLVAAVRRVIGEAIRDGEPKLFQAAKKIRMSPRRLQRRLKEY
                     GMDFKKLVDDTRRRLALQHIKEEKDSLTQIAFMLGYSDASAFNRAFKRWTGTAPLEYR
                     RTLVKQARTFG"
BASE COUNT          891 a          927 c          950 g          805 t
ORIGIN      
        1 attaccgccg agcggctcag acttttcgat tttactctgc cggtaagaga caatgtctcc
       61 gaaatcgtgg tgatgaacaa ggccgatcca gcaccggcta aacttgacga actcgccgga
      121 ctggaagtcc acgtgcgcaa aaccagcagc tactatgaaa gcctgcgcgg gctgaatgag
      181 cgcttccgac gggccggcag gccgcaaatg aaactaacgc tcgtgcccga tgagctcgag
      241 gacgaggacc tgatggagat ggtggcagca ggcttggtgc ggactatcgt cgttgatgat
      301 tggaaagccg ggctctgggc cgagatcctg cccaaaattc agcttcgccc tgagctggca
      361 ctgcgcacgg gggcgaagac cggctgggcg actcgcaaga acacaccgag gctcaaggca
      421 gtccttaacg aatttatcac cgagcatctt aaaggcacga accgggcggc gatccatctc
      481 gcaagctatc agcggcgttt caaggctctc cacgattcga cggctgctcc tgagtggagg
      541 aaattcgagg agacgatcgc ttattttgag aagtatggac aacagtacgg gttcgatcac
      601 ctgatgctgg cagcgcaagg atatcaggaa tcgcggctgg atcagcgcgc gcgtagccgc
      661 gtcggggcca tcggcattat gcagatcatg cccgcgactg gcaaagagct caatgtaggc
      721 gacatcagga acaccgaacc caatgttcat gggggagcga aatatatgcg gcggttgttt
      781 gatcaatatt ttaagaacgc aaactttgat gagcaaaacc gcgcgctgtt tgctttcgcc
      841 agctacaacg ccgggcccgg ccgcatcgcc aaggtccgcg ccgaggccaa agatcgacga
      901 ttggacccaa acaagtggtt caacaacgtc gaaatcgtcg cgtcgaatcg catcggtcag
      961 gaaaccgttc actatgtccg gaacatctac aaatattata cggcctaccg gctacaactc
     1021 gacgcgatcg cggcgcagcg tatggcaaag caacagatcg acagtaagac ggcaaagaac
     1081 tgaaattttt ccaaacgaag tggcatcatg ccagccagta tgacgatggc gtacgaagag
     1141 gcaatcaatc cattccgctt aagcgaccct aaactgtgtt cccgaacttc tttccttgtc
     1201 tctttcaatt ctcgaatact tgaaggggcg tcggagtaaa gaacgggtcg ctcgccgtaa
     1261 ctcaacagga cggaaaattc ttcggaagct tgcatggggc ggagaattga taccttcctc
     1321 agacactctc ttctccaaac actcggaact cgaagtctgg acggcttcgt gagcaatgcg
     1381 gcgccgtttc tgggatcgac agcgataccg tcgtcggttc gatacaaaaa gcagtcttcg
     1441 aattttacgc ggtgcaagaa aagacgctag gatgaatagg gccatgcatt gccggccaaa
     1501 gatcagaaaa tcgactacac gaggtcggct aaccagtcaa cggcagcttt tgagtcgcga
     1561 aacactgcga cgcgaagacc gtgttttcct aaacatgtgc tcacgtcgga tacactagca
     1621 tggtggttgt catccgacgg agaaaccaac gcaattttgc ttggcgatgg gtataggccc
     1681 ggccagcgcc tggaaaacaa acgatcaatc tgggtgggca caatggtgca gctagcgtcg
     1741 acgaaatcaa ttaaaacctt gcaatgggca cgagccaggc tcgctgctgc aactttgatg
     1801 agaatttgct ctaggctgcg gacgtttaat atcccttgag cgattataat catgtaatta
     1861 ccttctgccc aactgaactg cttgatctga atatgcatga aaaggttcca tcggtgttct
     1921 aggcaaacgc ttcccagtag aaaccacgga aattatctgc gacagtctcg ttcgccgggc
     1981 gtatcgtcca gcaatgtgaa cggaacaccc ctgcggaaaa attcaaactt gcagctgctc
     2041 cgactagaag cggttctggg agcgccgctg tgtacggtgt ctaatgggtg gaccgattgc
     2101 gtggctgcct cttaagccat gcgatcaact ctgtttcaag tacgcccggc gtagccgata
     2161 atgttacacg gcgaagaggc aaatcgtcgt gttttgccaa tcgtctaatc gagtcttcgc
     2221 tcagttcaaa aagtctttcg acgattcgca actgcaacca tcgatttgcg caagttgtca
     2281 tccgagtatt ggatcccgaa gcgaacactt gggtggttcg tggttggtcc cgtagatttc
     2341 gctcgacttt tcttagagtt tgtcatggta aggcttgaag acatcgctgt cttgacatgt
     2401 ggcgcctttt atctgacagc agaaaccatt tagggctttt atggcggggt caaaaaaccc
     2461 gatgatctcc atgtccgcgg cagccggact gctccaggca attgaagcgg ccggcgcgaa
     2521 tcccgatgac gttctcgaag cgatcgcaat ggatagggcg attttttcta acccggatag
     2581 ttttattagg gtttcgtcgt ttgcgcgaat tttggaagaa gctgcccgac tgagctcgga
     2641 tccatgcttc ggactgcatt ttggtgaaag ctacaatccc aagaacatag gtcccttggt
     2701 ctatgtggtt ttgaactcac caacgatcgc ggcaggcatc gaaaacgcag cgcggtatat
     2761 tacgattcac aatgaggcag ccagcgcgtc actcctagtc gaaaaccacc gtgcgtatct
     2821 tagattctta ccgaacgagc tcctgatcaa cagttcgcgc caacatagcg aatacgccat
     2881 gaccgttact cttaatacgt tgagattaat ggttggcagc caatgggcgc cgcaggaggt
     2941 tcatttcacc caccagctcc aggccgccac tccggagcac ttccgcgtgt tctgcgctcc
     3001 gatcatgttt ggctgttctg ccaatgctct tgttatggag agcgattttc tcaagcggga
     3061 ggtgccggca gcggacccga ggctctatca catactgaag cgttatctcg gaggcgtatt
     3121 gaaagatatg ccacgcgaag acgacctcgt tgccgccgtt cgtcgcgtga ttggggaagc
     3181 aatccgggat ggcgagccaa aactttttca agcagcaaaa aagattcgca tgagcccccg
     3241 tcgccttcag aggcgactca aagagtacgg gatggacttt aagaagctgg tcgacgatac
     3301 ccgccggcgt ttggcgctgc agcacatcaa agaagaaaag gactctctta cgcaaattgc
     3361 tttcatgctc ggctactcgg atgccagcgc gttcaatcgc gccttcaagc gttggaccgg
     3421 cacagcaccg ttggaatacc gtcgtactct tgtcaagcaa gcccggacat ttggctaaaa
     3481 caaaccgatg ttgacgggct tcttataaat tcaacgcatt cctgaagtag agtttgaatt
     3541 gctgattccc acttttactt tcgtcctcaa aat
//
LOCUS       DASUVS010000050         6086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_17584, whole genome shotgun sequence.
ACCESSION   DASUVS010000050 DASUVS010000000
VERSION     DASUVS010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17584"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VF182_02835"
     CDS             complement(<1..383)
                     /locus_tag="VF182_02835"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6436040.1"
                     /translation="MAAKRNYIIGVDTGGTFTDVVVLADTGEVWTAKASTTPDDFSRG
                     VMDAVGEAAQVMGVDSSDLLGRTTLFKHGSTVATNALITRSGVKVGLITTRGFEDTTE
                     IMRAIGRVDGLSDDEIKHVTWITKP"
     gene            complement(861..1562)
                     /locus_tag="VF182_02840"
     CDS             complement(861..1562)
                     /locus_tag="VF182_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6436041.1"
                     /translation="MLRVNGIHVYYDAIHALKGVTFSVEAGELVTLLGGNGAGKTTTL
                     KTISGLLRPRRGNIELEGCALDEIDPHEIVRRGVAHVPEGRKVFPRFTVLENLQIGGY
                     SRSMKSLVPDLDFVYGMFPRLHERQKQYAGTLSGGEQQMLAIGRALMAKPKLLLLDEP
                     SMGLAPKIVEQILENIRAINQSGVTVLLVEQNAAMALAISHRGYVLETGEVILSGTSS
                     DLAGNDLVRQAYLGG"
     gene            complement(1549..2328)
                     /locus_tag="VF182_02845"
     CDS             complement(1549..2328)
                     /locus_tag="VF182_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6436042.1"
                     /translation="MALLELRDISKRFGGLQALARIKLDIETGTIASLIGPNGAGKTT
                     LFNILTGVYQPDIGEIRFLDRPLMGLKPDKITKAGISRTFQNIRLFSHMTVLDNVLVG
                     MHSRMHTSFAEILLRTRNFHDRENGARSRAMELIDLTGLSGREGEWAHQLSYGEQRRL
                     EIARALAAAPKLLLLDEPTAGMNSTEAQSLVALFKVLVGKYVEAILLIEHNMRVVMGV
                     SHWVHVLDYGEKIAEGHPDEVRQNPRVIEAYLGAWAEHAQS"
     gene            complement(2328..3575)
                     /locus_tag="VF182_02850"
     CDS             complement(2328..3575)
                     /locus_tag="VF182_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6436043.1"
                     /translation="MSSSLPSLGLLAYIAASSVLAFVFPQSVLAFLLVESSLLLLCYV
                     PAPLKIKIVLAALVLLILIPFLGTFNGYYLEVAIQIGIYVALALGLNIVVGFVGLLNL
                     GFVAFYAIGAYLWAIFGSPQANEFIPGGYFPLSANWFFLFLILSIAVGALTGTLLGLP
                     VLRLRGDYLAIVTLGFAEVVRAMVNNLDKPINITNGPRGISPIVKPPLFFKPWLEFLG
                     LEIADATLYPLYFYFLILLMALLIILITRRLEDSRVGRAWKAIREDELAASAMGVPVV
                     RMKLFAFAVGASFACAVGVLFAAKQVFINPESFTFMESIGVLAMVILGGMGSIPGAIL
                     GATGVTILNLQLLKGLSLWLNGLRQSGAELTLPLLGSYSLSQLPAQFEPAKYERMVFG
                     LILILMMIYRPQGILPPRRNRSL"
     gene            complement(3572..4543)
                     /locus_tag="VF182_02855"
     CDS             complement(3572..4543)
                     /locus_tag="VF182_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008632793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6436044.1"
                     /translation="MLELALEILPQVLLEGLVLGFMYALIALGYTMVYGVLEFINFAH
                     SEIFVLGAFVGVEVLFWLQDAGQLAVLHPAFILSLVIAAGMLISGLAAMALERIAYRP
                     LQGSPRLVALISAIGASFFLQDALRLFESLWRNTFYLNYPTLDVLDQVLPLATTLVVP
                     VKSLMVIAISLLSMGGLYYFVNRTRIGTAIRAVAQDPETASLMGIPVNRVIALTFFVG
                     GAMGGLAGVLFGLHYSLVNPYSGFVPGLKAFTAAVLGGIGNIPGAMLGGMVLGLLEAF
                     AASYLSILTDGAFGAEYKDVFAFLVLITILIFRPRGLLGETVREGRA"
     gene            4729..>6086
                     /locus_tag="VF182_02860"
     CDS             4729..>6086
                     /locus_tag="VF182_02860"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX6436045.1"
                     /translation="MKKALIGSATVVALLIVTVIALPTFVDVGRFKSTYLPWLEETLQ
                     RRVDLGETRLTLLPRPSIKVSQLKISAGPSLRAQDFFTAQEVQLHFNLWPLLRGRFEI
                     TELMLEKPVLTLQTTSARRSGYTDLAGKKSSTAESNSIRPAPKQPGSDAVGLIIPRRL
                     RIKDGQLQIISEQRTPVRIHDIHVSMEEFSSERPFPYRATFNYPGLKTVTLTGELDYQ
                     HEQTKLILHDNCLTVQNLTLPLEGSISNVASMPRFDLVLAGDDVDARALTQIFAVFGL
                     APTDTEISGPIGLRIAVNGPSNNLMTEIQGQFKNVELYGKRGIKGNLNGAILLRLNAA
                     GGADLMKQLQGNGKLSAREGELTHVDLVKKVQRATGAIGFSEHQRREATTFRTLDTEF
                     TLSNGVVDFKRIYLVNPHLELNGGGTMTLGRQALNIGMDATLSPQASSKASRGRTSQL
                     LK"
BASE COUNT         1536 a         1740 c         1597 g         1213 t
ORIGIN      
        1 tcgggtttgg taatccaggt aacgtgtttg atctcgtcat cggataatcc atccacacgg
       61 ccgatggcgc gcatgatctc ggtggtatct tcgaaccccc gagtcgtaat cagacccacc
      121 ttgacgccgc tgcgggtaat caatgcgttg gttgcgaccg tgctgccgtg cttgaacagc
      181 gtggtgcgac cgagaagatc cgaactgtca actcccatga cctgcgcggc ttcgcccacc
      241 gcatccatta cgccgcggga gaagtcgtcc ggcgtggtcg aagcttttgc cgtccaaact
      301 tcgcctgtgt ccgccaagac gacaacatcg gtaaacgtgc cgccggtatc gacgccgata
      361 atgtaatttc tctttgccgc catgaaattc cttccgaagt gaattgtttg cagaaatatg
      421 agcccgagcg tgccttgtca agtgtgagtg aacctgaatt gcgccgctcg aagtgatacg
      481 gcaattgagc ggcgagcccc tccagagcaa aggccatggg cctctggctg atagcgatcc
      541 gcaagctcca gtgctttagt ggacgactga aaaaaatgtc agcccaggac caagcaccat
      601 acacggcatt gaaattgttc acttctgcgc cttcgctcct ccagcctcac ctctcaatcg
      661 gtacgaagtt gctcatctac tatggaattc gggggttgaa tgaagcgcgc ggctaaaagg
      721 cgtaggctgt ctaaaaagtt cacaggcgag gagcgcaaaa tcacaaggat gaaagcggaa
      781 tctgaaagcg gaattcaatt ttcagttttc atctttcata attcatccgt tcaagcctgt
      841 gagatttttc agcagccctg ctagccgccg agataggctt gtcgcactaa atcattgccc
      901 gccagatcgc tcgatgtgcc gctaagaatc acttctcctg tctccagcac ataaccgcga
      961 tgggaaatcg ccaaggccat cgcggcattt tgctcgacca gcagaaccgt gacgccggat
     1021 tggttgatcg cgcgaatgtt ctccaaaatc tgctcgacga tttttggcgc cagtcccatc
     1081 gatggttcat ccaacagcaa caattttggc ttggccatca aggcccgccc gattgccagc
     1141 atctgctgct cgcctccgga cagcgttcct gcgtattgct tctgccgttc gtgcaagcgc
     1201 ggaaacatcc cgtaaacaaa atcgagatca ggcacaagcg acttcatgct ccggctgtat
     1261 ccgccgattt gcaaattctc cagcactgta aaacgaggaa agacttttcg gccctccggc
     1321 acatgagcca ctccgcgccg gacgatctcg tggggatcga tctcatccaa tgcgcagcct
     1381 tccagttcga tgttgccgcg gcgcggccgc agcagtccgg aaatggtctt taaggtggtg
     1441 gtcttgccgg cgccgttgcc gcctaaaagc gtcacgagct cgcccgcttc gaccgagaac
     1501 gtaactccct tgagcgcatg gatggcgtcg taatagacgt ggatgccgtt aactctgagc
     1561 atgctccgcc caggcgccaa ggtaggcttc gatcacccgc ggattttgcc gcacttcgtc
     1621 aggatggccc tcggcgatct tttcaccgta atcgagaaca tggacccaat gcgatacgcc
     1681 catgaccacg cgcatattat gctcgatgag cagaatcgcc tcgacgtatt tgccgaccaa
     1741 aactttgaat aaggcgacca gcgactgcgc ttcagtcgaa ttcatccctg cagtcggctc
     1801 atcgagcaac agcagcttcg gcgctgcggc caaggcgcgc gcgatctcga gccgccgttg
     1861 ttctccgtag gaaagctggt gcgcccactc gccttcacgg ccgctgagtc cggtcaaatc
     1921 aatcagttcc atcgcccggc tgcgggctcc gttctcacga tcgtgaaagt tccgggtacg
     1981 cagcaggatt tcggcaaaac tcgtgtgcat tcgcgaatgc ataccgacta agacgttgtc
     2041 gagaaccgtc atatgcgaga acaggcggat attttgaaac gtccggctta tacccgcctt
     2101 ggtgattttg tcgggtttta gacccattaa aggccggtcg agaaaacgga tttcgccgat
     2161 atccggctga taaaccccgg taaggatgtt gaacagcgtc gtcttgcccg cgccgttggg
     2221 gccgatcagc gacgcgatgg taccggtttc gatgtcgagc ttgatccgcg ccaaagcttg
     2281 tagcccgccg aaccgtttgg aaatatctcg cagctccagc agcgccatta aagtgaccgg
     2341 ttacgtcgcg gcggcaagat accttgcggg cggtaaatca tcatcagaat caagattaga
     2401 ccgaaaacca tccgttcata ttttgccggc tcgaattggg ccggcagctg gcttaatgaa
     2461 tagctcccca aaagcggcaa ggtcaattca gcccctgact ggcgcaggcc gttgagccag
     2521 agcgacaatc ctttgagcaa ctgcaggttg aggatcgtca caccggtggc gccgaggatc
     2581 gcgcctggaa tcgagcccat accgccaagg atcaccatgg ccagaactcc gatggattcc
     2641 atgaaagtaa aactctcggg attgatgaaa acctgcttgg cggcaaacag cactccaacg
     2701 gcgcacgcga acgacgcgcc caccgcaaat gcaaacagct tcatgcgtac gaccggaaca
     2761 cccattgccg acgccgccaa ttcgtcttcc cggatcgcct tccacgcccg cccgacgcgt
     2821 gagtcctcga gacggcgtgt gatgaggatg atgagcagcg ccattaacag gatgaggaag
     2881 taaaaataca gcgggtacaa tgtcgcatcg gcaatttcca accccaaaaa ttccagccag
     2941 ggtttgaaaa acaacggtgg tttaacaatc ggggagatgc ccctgggacc attggtaatg
     3001 ttgatcggct tatcgagatt gttgaccatt gcgcgcacca cctcggcaaa ccccagggtg
     3061 acgattgcca gataatcgcc gcgcaaacgc agcaccggca ggccgagcaa agtgccggtc
     3121 agcgctccta cagcgatact taaaatgaga aacaggaaaa accaattggc cgacaacgga
     3181 aagtatccac cgggaataaa ctcattggct tgcggcgatc cgaagatcgc ccagagataa
     3241 gcgccgatgg cgtaaaacgc aacgaagcct agattgagca agccgacaaa gccgaccacg
     3301 atattcaatc ccaaagcgag cgcgacatag atgccgattt gaatggcgac ttcgagataa
     3361 tagccgttga aggtgcccag gaacggaatc aagattagca gcaccaatgc agccaagact
     3421 atttttatct tgagcggcgc gggcacgtag caaagcagca gcaatgaaga ttccacgagg
     3481 agaaaggcca agacagattg gggaaagaca aaggccagca cggagcttgc ggcgatgtac
     3541 gccaataagc ccaacgacgg cagcgagctg ctcacgcccg gccctcgcgc acggtctcgc
     3601 caagcaaacc tctgggacga aaaatcagaa tcgtgatcag cacaagaaat gcgaagacat
     3661 ccttgtattc ggcgccgaag gcgccgtcgg taagaatcga caaatacgaa gcggcgaatg
     3721 cctccaataa accgagcacc atgccgccta acatcgcgcc gggaatattg ccgatgccac
     3781 ccaggaccgc cgcagtgaaa gccttcagtc ccggcacaaa cccgctgtag ggattgacca
     3841 gactgtaatg aagtccaaac aaaaccccgg ctaaaccacc cattgcgccg cctacaaaga
     3901 atgtgagcgc aatcacgcga ttgaccggaa tgcccatcag cgaggcggtt tccggatcct
     3961 gggcgacagc gcgaatcgcc gtgccgatgc gcgtccgatt gacgaagtag tagaggcctc
     4021 ccatgctcag cagcgagatg gcgatgacca tcagactctt caccggaacc acaagagttg
     4081 ttgccaacgg caacacctgg tcgagcacat ccagagtcgg ataattgagg taaaaggtgt
     4141 tgcgccagag gctttcgaac aaacgcaatg catcctggag aaaaaaagac gcgccgatgg
     4201 cagagatcag cgccaccaga cgcggactac cctgaagcgg acgataggcg atccgctcca
     4261 gagccatggc ggctaggccg ctaatcaaca tgccggcagc gatcaccagg gacaaaatga
     4321 aagccggatg caacactgcc agttgtccgg cgtcctggag ccaaaacagc acctcgacac
     4381 cgacgaacgc gccgagaacg aagatttcac tgtgagcaaa gttgataaat tccaagacac
     4441 catagaccat ggtatagccc agagcgatca acgcatacat gaatcccagt acgagaccct
     4501 ccaacaacac ctgtggaaga atttctagcg ccagttccaa tatgtcagcg gcctttcgaa
     4561 gtttgttacc ctaacaaata ctgcgcttag tggctactct tcgcaacgat ttagccactg
     4621 atgccgtcat tgaatccggg ctgcggtccg tataatgtcc cggaaaatga cgacactcat
     4681 cctagggaac tgcgttgaaa cttgcgtagt cgagccggag acggttttgt gaaaaaagcc
     4741 ttgatcggca gcgccactgt tgttgctctc ttgattgtaa cggttatcgc gttgccgacg
     4801 tttgtcgatg tcggccgctt caaaagcact tatctgccat ggctggaaga aactctgcag
     4861 cggcgagttg acctcggcga gacacgctta actctcctgc caagaccttc gatcaaagtt
     4921 tcgcagctga agatctcggc cggcccaagt cttcgcgccc aagacttctt caccgcgcaa
     4981 gaggttcaac tgcacttcaa tttatggccg ctgctgcgcg gccgtttcga aatcaccgag
     5041 ttgatgctcg aaaagcccgt tttgacactg cagacaacat ccgctcgacg ctctggctat
     5101 actgatctgg cgggcaaaaa gagttcgaca gccgagtcaa atagcatccg tccggcaccc
     5161 aaacagccgg gcagcgacgc cgttggtttg atcattcccc gccggctgcg gatcaaagac
     5221 gggcaattac agattataag cgaacaacga acgcccgtgc gcatccatga tatccacgtt
     5281 tctatggaag agttctcaag cgaaagacct tttccgtacc gcgccacttt taattatcca
     5341 ggcttaaaaa ccgttacatt gaccggagag ctcgattacc agcatgaaca aaccaaactc
     5401 atccttcacg acaattgtct gacggtgcag aatctgaccc tgcccctgga gggaagcatc
     5461 agtaatgtag cctcgatgcc gcgcttcgac ttggttctgg ccggtgatga cgtcgatgcc
     5521 agggcgttga cgcagatatt tgcagttttc gggttagcac cgacagacac cgagatttcc
     5581 ggcccaatag gcttgcgcat tgcggtgaac ggcccgtcga acaatctgat gacagagatc
     5641 caaggccaat tcaaaaatgt ggagttgtat ggcaaacgcg ggatcaaagg aaatctaaac
     5701 ggcgcaattt tgcttcgctt aaatgccgcc ggtggcgccg acctgatgaa gcagttgcaa
     5761 ggaaatggca agctatcggc gcgcgagggc gaattgaccc acgtcgatct cgtaaaaaag
     5821 gttcaacgcg cgaccggcgc catcggcttc tccgagcatc aacggcgcga agccaccaca
     5881 ttcaggaccc tggatacgga atttactttg agcaacgggg tcgtcgactt caaacgaatc
     5941 tacctagtaa acccgcacct ggaactcaat ggcggcggca cgatgaccct cgggcgtcag
     6001 gcgctcaata tcgggatgga tgcaactctc tcccctcagg cttccagtaa agccagccgg
     6061 ggcagaacca gccagcttct caaaaa
//
LOCUS       DASUVS010000051        12343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1111321, whole genome shotgun sequence.
ACCESSION   DASUVS010000051 DASUVS010000000
VERSION     DASUVS010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12343
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111321"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VF182_02865"
     CDS             <1..967
                     /locus_tag="VF182_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6436046.1"
                     /translation="VFFFSSAYGQSEDELVAGAKKEAKVTFWSGMALDDSRLVAAGFE
                     KKYPFIKVDIFRAGGEQIVNRAIAEAGAGQNTSDVLNAFALKVLQERNFLQPYAASEA
                     AAYPAAYKDPKNFWVSLKNNYNVIGYNSRLVSPQEAPRNWEDLLHPRWKDKLGMDDEE
                     YFWYAGMLKHWGEAKGKKYMEALAKQGIQWRNGHNLLVELMGAGEYPVSVVIYPERAE
                     QLKAKGQTVEWVKTTDPILVNMDIMGIAAKAPHPNAAKLFFNYVLSKEAQMILHSRHR
                     ISARTDIPPLMASMEPKKLPLVPLDPAIPTNASVVREFRSVFGLK"
     gene            1138..1845
                     /locus_tag="VF182_02870"
                     /pseudo
     CDS             1138..1845
                     /locus_tag="VF182_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
     gene            2051..2689
                     /locus_tag="VF182_02875"
     CDS             2051..2689
                     /locus_tag="VF182_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEX6436047.1"
                     /translation="MYDIAGKKIPSTLGEIVEPAHTALLIWDMEYAICPNAFNYQDMV
                     PKLKEVSSLARRVGAPVFYSMQTGFDLAKEEAGVWVRIRMKRAKASDPSQLLNQKDDP
                     HDREIIAELKPQPQDIVFQKRRPDAFVGTDFDLMLRSNGIKTIMISGVATEGGVEGTA
                     RSARNLGYDVVILKDCVGSRSRDLHEMALKLMEGSFFDITTAVEIAAIWGEK"
     gene            2742..3716
                     /locus_tag="VF182_02880"
     CDS             2742..3716
                     /locus_tag="VF182_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEX6436048.1"
                     /translation="MKIAVIDDYQNAFRTLKSYPKLSDHEVVVFTDTETDIGKLADRL
                     KDADAVVLTQQRSSFPRALIERLPKLKLIGQTGRAATHVDLDACTEKGIVVSAGGTGN
                     SNATAELTWGLILSALRNLPLEVKRLKEGHWQSTLGIGVNGKTLGIYAYGKIGSIVAA
                     AGKAFGARVVCWGREGSTARAKAAGFEVAESREDFFGRADILSLHLPLNKDTQGIVTR
                     DDLGRMKPTALIVNPSRAGLIAPGALVEALKAGRPGMAAVDVYEKEPVIGADHPLLKM
                     NNVTCTPHLGYVTREGYEEYYAVVVDDILAFAAGKPQNVLNPQAIAKK"
     gene            complement(3891..4952)
                     /locus_tag="VF182_02885"
     CDS             complement(3891..4952)
                     /locus_tag="VF182_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6436049.1"
                     /translation="MSDGHPLVETPELAALHRRLAEYSLGGHWQPREKNPELVAHVWP
                     WSVIYSCLMESGEVVKLGGIDDAAKRRTVQLVNPSLTNKKATTRTLQMSIQLVKAGER
                     AECHRHTAAALRFVVEADGTAYTNVEGEQMLMEPGDLVLTPNWTWHDHFNPGEKNVVW
                     LDVLDIHLSNHLDAVFQENYGEGPAQPVVKADGYCKQSLGAVRPRMKSITNNAALPYT
                     YKWRDTLRALQDIAGAGGNDPHDGVLLEYVHPLTGGPTMPTIGCWIQLLRPGETTKPH
                     RHTSSTIYHVVQGEGLTTVGPKKTAGKELSWSERDCFFVPSWKWHQFQNTSKRDPAII
                     FSVTDRPVLESLGLFREEH"
     gene            complement(5408..6931)
                     /locus_tag="VF182_02890"
     CDS             complement(5408..6931)
                     /locus_tag="VF182_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX6436050.1"
                     /translation="MLAKINSAALYGIDALRVEVEIDLASGLPQLATVGLPEGAVKES
                     KDRIRAAVKNCGYTFPAKRITINLAPADIKKEGSAYDLPMAIGILAAEGSVDKNLIND
                     YFLIGELSLDGAIKPVRGALPIAMAAKREHVKGLILPKHNAPEAAVVSEIEVLGVSDL
                     PEVVEFLNGRRAIERTQVNLEALFRQQSQYSSDFTDVKGQQHVKRALEVAAAGGHNVI
                     MVGPPGSGKTMLANKLPTILPDLTLAEALETTKIHSVMGLLNGSPIIATRPFRYPHHT
                     ISDAGLVGGGTVPKPGEVSLAHNGVLFLDELPEFKRNVLEVMRQPLEDGSITVSRAIG
                     SINYPAGFMLVAAMNPCPCGFFTDPQKECVCTPLQIQRYRSRVSGPLLDRIDIQVEVP
                     ALRYQDLASKDAGESSDSIRQRVNAARAIQLERFKKRNIHGNAQMGSRDIKRHCLLKE
                     DAEKLLENAINRLGLSARAYSRVLKVGRTIADLAGSEEMQSSHIAEAIQYRSLDRRM"
     gene            complement(6973..7227)
                     /locus_tag="VF182_02895"
     CDS             complement(6973..7227)
                     /locus_tag="VF182_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6436051.1"
                     /translation="MLITASRMPLYEYECSQGHRFEAIQKVSDKPLRKCTTCAAAAKR
                     AISRPTLLHNRGVHVFDRVSKDDALRTRPSSLKSFKKDKF"
     gene            7374..8840
                     /gene="guaB"
                     /locus_tag="VF182_02900"
     CDS             7374..8840
                     /gene="guaB"
                     /locus_tag="VF182_02900"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX6436052.1"
                     /translation="MLEQEVLPVGLTFDDVLLIPAESAVLPRDTDVSTRLTERITLNI
                     PLVSAAMDTVTESKTAIAMAQEGGMGIIHRNLPVGIQAGEVEKVKKFESGMISDPITV
                     APDQQISEAKVIMDKYGISGLPVKRDGRLVGILTNRDLRFEKKLDRTVSEVMTKDKLV
                     TVKEGVDLDEAKELLHRHRIEKLLVVDDNFNLKGLITVKDIEKRTQFPSACKDTRGRL
                     RVGAAVGVGADWEERVEALVRAGVDLIAIDTAHGHSKNVLDTIRQIRGRYPDLDLSGG
                     NVATAEGTTALIQAGVNVVKVGVGPGSICTTRVVSGVGVPQVTAIGSCAKAAARHNVP
                     IVADGGIKFSGDITKALAAGADSVMIGSLFAGTEESPGETILYQGRTYKVYRGMGSLG
                     AMKQGSKDRYSQGDVEEAMKLVPEGIEGQVPYKGSLSFNIHQLVGGLRAGMGYLGCRD
                     VATLRAKARFMQITAAGLREGHVHDVFITKEAPNYSVE"
     gene            8837..10369
                     /gene="guaA"
                     /locus_tag="VF182_02905"
     CDS             8837..10369
                     /gene="guaA"
                     /locus_tag="VF182_02905"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX6436053.1"
                     /translation="MILVLDFGSQYTQLIARRIRESHVYCEIHPFSVGLDKIAELNPQ
                     GIILSGGPASVYQENAPKVKPELFDQTVPFLGICYGMGVINLASGGEVARADDREYGP
                     ADLMIDSNADLFYGFKKNDPVRVWMSHGDKMTVIPPGWSSLAHSGNSPIAAFVDPTRQ
                     FFGVQFHPEVAHTPRGKEILDNFVFRICGCKPDWTMEHFIEASVQKIRDQVGTGRVLC
                     ALSGGVDSSVAASLVYRAIKDRLVCVFVNNGLLRQGEAERVCQLFSERFGESFRYVDA
                     SEAFLSDLSGIEDPEIKRKKIGYRFIEVFESEAEKLGEIDFLAQGTLYPDVIESVSFV
                     GPSVTIKSHHNVGGLPAAMKLKLIEPLRELFKDEVRILGKELGLPHEMIHRQPFPGPG
                     LAIRILGPVTEERLRIIRAADTIVWHEMRAARLYEKVWQSFAVLLPVKTVGVMGDERS
                     YENVIALRVVESLDGMTANWVPLPADLLSRISNRIINEVRGVNRVVYDISSKPPATIE
                     WE"
     gene            10414..10842
                     /locus_tag="VF182_02910"
     CDS             10414..10842
                     /locus_tag="VF182_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX6436054.1"
                     /translation="MKEISQVLLFDSDRRLLVYLRDNNPAIPFPNHWDFFGGHLEAGE
                     TPERALVREVKEELGVELKQWKFFRLYHCTRGDAYPNLKHIYWAKIDQSAAQLTLNEG
                     QRLMSIGLEERDRVKFANILAQILEDFIGSGLWPERGLHS"
     gene            10905..>12343
                     /gene="dnaE"
                     /locus_tag="VF182_02915"
     CDS             10905..>12343
                     /gene="dnaE"
                     /locus_tag="VF182_02915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX6436055.1"
                     /translation="MEHSRFVHLHCHTEYSLLDGANKVEKLFKRIKTLKQPAVAMTDH
                     GNMFGAVEFYREAISHGIKPIIGCEIYVAPTSRFEKKGVDRGPKEYNNHLILLAMNGE
                     GYRNLCKLVSLGYMEGFYYKPRIDKELLGELNGGLIALSACLQGEVSQALSYGNVEKA
                     KTAAASYLSIFGDRYYIEVQDNKLAEQEKVNRLLVEMAKDLSIPVVATNDCHYGERDD
                     FHAHDVLLCVQTGKTVTDDNRLKLDTDELYLKSAEEMIGGFDYCPGAVERTLEIAERC
                     NVEMEFGKYHFPNYTPPKEISLDDYLDELARSGLEERLAGVVEPGLRSSYVERLEYEL
                     EIIKRMQFPGYFLIVADFINYAKQNGIPVGPGRGSSAGSLVAYALKITDLDPIRHVLL
                     FERFLNPERRSMPDIDVDFCIRGRAQVIQYVKEKYGADRVAQIATFGTLKAKAAIKDV
                     GRALGFTFAETDMIAKLIPAPKQGFDYPLT"
BASE COUNT         2885 a         3166 c         3512 g         2780 t
ORIGIN      
        1 ggtatttttc ttcagcagcg cttatggcca atccgaggat gagctcgtag ccggagccaa
       61 gaaggaggcg aaagtcacct tctggagcgg catggcgttg gatgactcac gcttggtggc
      121 cgccgggttc gagaaaaaat atccgttcat caaggtcgat attttccggg cgggaggcga
      181 gcagattgtc aatcgcgcga ttgccgaagc gggcgccgga cagaacacat ccgatgtgct
      241 caatgctttc gctttgaagg tgctgcaaga aagaaatttt ttacaaccgt acgccgcctc
      301 cgaagcggcc gcttatccgg cggcctataa ggatcccaag aacttctggg tatcgttgaa
      361 gaacaattac aacgtcatcg gctacaactc gaggctggtc agtcctcagg aagccccgag
      421 aaattgggaa gatctgctgc atccgcgttg gaaagacaag cttggcatgg atgacgagga
      481 gtacttctgg tatgccggaa tgctcaaaca ttggggcgaa gcgaaaggga aaaaatacat
      541 ggaagccctg gccaagcagg gcattcaatg gcggaatgga cacaacttat tggtggaact
      601 gatgggcgcc ggcgagtacc ccgtttcagt ggtgatttac ccggaacgcg ccgaacaact
      661 gaaggccaaa ggccagacgg tcgaatgggt gaaaaccacc gatccgattc tggtcaatat
      721 ggatatcatg ggaattgcgg ctaaagcgcc gcaccctaat gcggcaaagc tattttttaa
      781 ctatgtgctt tccaaagaag cgcagatgat tcttcacagc cgccatcgga tctcggcgcg
      841 caccgacatc ccgccgctga tggcgagcat ggagcccaag aagcttccct tggtgccgct
      901 cgatccggca attccgacga atgcgagcgt cgttcgggaa ttcagatctg tcttcggatt
      961 gaagtaaggt gatcttaatc agacaaccca aatagcaagc taaggatttg cacgtctgct
     1021 ggcgatgata ccttgcgcgc atttagccgg ccgaaaggag aggacgtgac catggaaatc
     1081 acaagtgaca agttcgtcct gttcacccag cctggttgaa gcagttgcct tagaacgaaa
     1141 gagtttcttt cgaaaaaagg catcgaattt tccactatcg acgttttgaa cgacccggcg
     1201 gggcgagagc agttgctcag cttgggggcg cagagtgtgc cggtgctgtc aaaggatggt
     1261 cagttcatct tctgccagaa catggaagac gtggctgaat ttgtcggact gcaaggtact
     1321 ggacacagcc cgctttcgcc gcaagcattg ttcgacaagt ggatcaccgt gctgcgcgca
     1381 gcgcaacgtt acgtacttca gattcccaac ccgcgactga cggagcgcgc cattgaaaat
     1441 cgcgaccgct cgatccggct tcttagccat cacatctttc gcatcggcga agctttttta
     1501 gataccgcgt tggagaatgt cgaataccgc accaacaatg ccaacgtgcc gcccgccgac
     1561 ggaagctgcc tgacgggtgt cgagattgcg gcttatggcg cagggattat cgaacggatc
     1621 gagaattggt ggatccgact cgaagacaaa tcctgccggc aaaaggtcag gacattttat
     1681 ggtacgccgc cgatgcacca actgtttgag cgctcaacct ggcactcggc acagcatgcg
     1741 cggcaaatga tcgccgtgct cgaacgcttc ggtatcgaac ctgacaaccg gttaactgcc
     1801 gaggatcttg ccggactccc tctcccggaa gggatttggg agtagagagc ctcaaagtgt
     1861 ttcaccatga ggtttcaaaa atcttacttc agcttcgtgt aaggtgatgc gcatgacgcc
     1921 aggacgatct catgcttgcg cttctgacat tggtgaattc ccgccgcgca atctcctgtt
     1981 gacgacgagc tttgctttcg actaacttcc agcgatagtt ggtccagtcg aacgataacc
     2041 gaggtgaatc atgtacgaca tcgccggaaa gaaaattccc agcacattag gagagattgt
     2101 cgagcccgcg cacacagctt tgctcatttg ggatatggaa tacgccatct gccccaacgc
     2161 tttcaattac caggacatgg tgcccaagct gaaggaagtg tcctccctgg cgcgccgggt
     2221 tggcgcgccg gttttctatt caatgcagac cggctttgat ctggccaaag aagaagcggg
     2281 agtttgggtg cgcatcagga tgaagcgggc gaaagccagc gatccgagtc aactcttgaa
     2341 ccagaaggac gatccgcacg acagggagat cattgcggag ttgaaacccc agcctcaaga
     2401 catcgttttt caaaagcggc ggccggacgc ctttgtcggg acggatttcg acctgatgct
     2461 cagaagtaac ggcatcaaaa cgattatgat tagcggcgtc gcgaccgaag gcggtgtcga
     2521 aggcacggcg cgaagcgcgc gcaatctcgg ctatgacgta gtgattctga aagactgcgt
     2581 cggctctaga agtcgcgatt tacacgagat ggcgctgaag ctgatggagg ggagcttttt
     2641 cgatatcacc accgcggtcg aaatcgccgc tatctgggga gaaaagtaaa ccctcagagt
     2701 atctaccagc ttcataaaat aagatgaaag ggttgcattc gatgaaaatt gccgtgattg
     2761 acgattatca gaacgcattc cggacattga agtcttaccc gaagctcagt gaccatgagg
     2821 tcgtggtatt taccgataca gaaacggata tcggcaaatt ggccgacaga ctcaaagacg
     2881 ccgatgcggt ggtgctcacg cagcaacgat cgagttttcc gcgggccttg atcgaacgat
     2941 tgccaaagtt gaaactgatc ggtcagactg gacgcgccgc gactcacgtg gatctcgatg
     3001 cctgcaccga gaaggggatc gtggtttccg cgggcggcac cggcaactcg aacgcaaccg
     3061 cagagctgac ctggggactc attctgagcg cgctgcggaa tcttccgctt gaagttaaac
     3121 ggctcaagga gggtcattgg caatcgacgt tgggcatcgg cgtcaatggc aagacgttgg
     3181 gtatttacgc ttacggcaaa atcggcagta tcgttgccgc cgccggcaaa gcattcggcg
     3241 cacgagtggt gtgttggggc cgcgaaggct ccaccgctcg ggccaaggcc gccgggtttg
     3301 aagtagccga gagccgcgaa gacttttttg gccgggcgga tattttgagc ttacatctgc
     3361 cgctcaacaa agacacccaa gggattgtca cccgcgacga tctcggccgt atgaaaccga
     3421 cggcgctgat cgtcaaccca agccgcgcgg ggctgattgc ccccggcgcc ttagtcgaag
     3481 cgctcaaagc cggccgcccg ggtatggctg ccgtggacgt ctacgaaaaa gagccagtga
     3541 tcggcgccga tcatccgctt ctaaaaatga acaatgtcac ctgcacgcct catttaggtt
     3601 atgtcaccag ggaaggttac gaggaatatt acgctgtggt ggtcgatgat attcttgcct
     3661 tcgccgcagg caagccgcaa aacgtgctca atccgcaagc gatcgcgaag aaataatcat
     3721 cccgcgagga ttgcaccgaa gcgttggtga ttggtatatc gatgccgctg aaagacatcg
     3781 atgccgcaat tgcggcagtc actctaacgt taacattcga tggcgcggac ccgatattgg
     3841 aacgaagcgg gcaagcgcaa cggagtttgt gagccttttt cagctgccca ttagtgttct
     3901 tcccggaaca accctaagct ttccaacacc ggccggtccg tgaccgagaa gatgatcgca
     3961 gggtcccgct ttgacgtatt ctgaaactgg tgccatttcc atgacgggac aaagaaacag
     4021 tctcgttcgc tccagctcag ttcttttccg gcggtttttt tcggacccac ggtcgtaaga
     4081 ccttcgccct gaaccacatg ataaatagtg ctgctggtat ggcggtgcgg tttggtagtt
     4141 tcaccaggcc ggagtaactg gatccaacaa ccgatagtcg gcatggtcgg gccaccggtc
     4201 aggggatgaa cgtactcgag gagaactccg tcgtgcgggt cgttgccgcc ggccccggcg
     4261 atgtcttgca gcgcgcgcaa ggtatcgcgc cacttatagg tgtacggcag cgccgcatta
     4321 ttagtgatgc tcttcatccg cggccggacc gcgcccaggc tttgtttgca ataaccatcg
     4381 gctttgacta caggctgggc gggaccttcg ccatagttct cttggaacac cgcatcaaga
     4441 tggttgctca aatgaatatc gagcacgtcg agccagacaa catttttctc tccaggattg
     4501 aagtgatcgt gccacgtcca attgggcgtg agcactagat cgcccggctc catcagcatc
     4561 tgttcgcctt cgacattggt gtacgccgtg ccgtctgcct caaccacgaa tcgcagcgca
     4621 gccgcggtat gtcgatggca ctcggcgcgc tcgccggctt tgaccagttg tatcgacatc
     4681 tgcaaagtgc gggtggtggc ttttttgttg gtcaatgatg gattgaccag ctgaacggtc
     4741 cgacgctttg cagcgtcatc gatgccgccc aacttcacga cttcaccgga ctccatcagg
     4801 catgaataaa tcaccgacca aggccagacg tgagcgacca gctccgggtt cttctcgcgc
     4861 ggttgccagt gacctcccaa cgaatattcc gccaatcgcc ggtgtaaagc tgccagctcg
     4921 ggagtttcaa ccaatggatg tccgtcgctc atgggatttt ctcctgttta gcgaattatc
     4981 gctccgctga agtgcgccga agcttcggta ctctaacgcc aggtaaaata ccgtgtcaaa
     5041 cgcaccagga ttctcccctt caattgtgct gggactgcgt gataattctt agcgggggtt
     5101 gttgtcttgc cgaacacaaa aaacttgaca tggtgcgggg ccttagaggt cgcatcaaga
     5161 aagacctccc actctcgtgt ggagcgcagc cgacgagttg aaggatcgta tggcttacgt
     5221 tttggcgctt cagcgaaggc gacggttccc cggaatgcat gacagaggcg cacagcaaaa
     5281 catcgataat gtggttgacg agatgcgcgt ttagttcatg agatctgcaa gacgtccaga
     5341 ttgactagaa aaaccctggg cagaccaggt aaacggctat tgcaaaggaa atgatcgccc
     5401 gcattcgtta cattcgtcga tccaagctac ggtactgaat cgcttcggcg atgtggctcg
     5461 actgcatttc ctcagagccg gcgagatccg cgatggtgcg gccgactttg agcacacggc
     5521 tgtacgcacg agcgctgagg cccaagcggt tgatggcgtt ttccaggagc ttttcggcat
     5581 cttctttgag caggcaatga cgctttatgt cgcgtgagcc catttgcgca ttgccgtgaa
     5641 tatttctctt tttgaaccgc tctaactgaa tggcgcgcgc ggcattgacc ctttggcgaa
     5701 ttgaatcgga actttcgccg gcgtctttac tggcgagatc ctggtagcgc agcgccggca
     5761 cctcgacttg aatatcgatg cggtcgagca acggcccgga tacccgggaa cggtaacgct
     5821 gaatctgtaa cggcgtgcag acgcactcct tttgcggatc ggtgaaaaat ccgcaggggc
     5881 acggattcat agcggcaacc agcatgaagc cggcggggta attaatcgaa ccgattgctc
     5941 gtgatacggt gatgctgcca tcctcgagcg gctgacgcat aacctcgagg acattgcgtt
     6001 taaactcggg caattcatcg aggaagagca cgccgttgtg cgctagcgaa acttcgccgg
     6061 gcttaggcac ggtcccgccg ccgaccaaac cggcatcgga gatggtatgg tgcgggtaac
     6121 gaaacgggcg ggtggcgatg atcggcgaac cgttgagcaa gcccataacg ctgtggatct
     6181 tggtggtttc aagcgcttcg gcaagtgtca ggtctggcag gattgtcggc agtttatttg
     6241 ccagcatggt cttacccgag ccgggtgggc ccaccataat cacattgtgg cctcccgctg
     6301 cggcaacttc taatgcgcgc ttgacgtgct gctggccttt aacgtcggtg aaatccgagc
     6361 tgtattgcga ttgctggcgg aacagagctt caaggttgac ctgggtgcgc tcaatggcgc
     6421 ggcgaccgtt taaaaactct accacttcag gaaggtcact gacgccaaga acttcgatct
     6481 cgctgacgac tgcggcttca ggcgcgttgt gcttgggcag gatcagccct ttgacatgtt
     6541 ctctttttgc cgccatggca atgggcaacg cgccacgcac aggcttgatc gcgccatcga
     6601 gcgatagctc gcctatgagg aaataatcgt tgattagatt tttatcgacc gaaccttccg
     6661 cggcaagaat accgatggcc atcggcaggt cgtaggccga gccttctttc ttgatatcgg
     6721 ctggcgcaag attgatcgtg atgcgcttgg cgggaaaagt gtagccgcag ttcttgacgg
     6781 cggcgcggat ccgatctttg ctttctttga ccgcgccttc aggcaacccc accgtcgcaa
     6841 gctggggcag gccggaggcc agatcgattt ccacttcgac gcgcaacgcg tcgatgccgt
     6901 atagggccgc gctgttgatc ttcgctagca taaaccgccg gctacgctag caaaggcgcc
     6961 acaggataac aactagaatt tatccttctt gaaggacttg agcgatgatg gccgcgtgcg
     7021 aagcgcgtca tctttgctga cccgatcgaa gacgtggacg ccacgattgt ggagtaatgt
     7081 agggcgagag atcgcccgtt tggcggcggc ggcgcaggtc gtgcacttcc tgagcggctt
     7141 atcgctgact ttttgaatcg cttcgaagcg atggccttga gaacattcgt attcgtaaag
     7201 cggcatcctg ctggcggtaa ttagcatatt aaaaatcgtg tcacaatgaa gcgactagtt
     7261 tgtctgatag aaggtttcct ggtataagat tctatggttg tcgcgggtcg atcgatcatt
     7321 ccacactacc aaccacaggt ctcactgtta ttaatcttgc tcggaggtga gagatgttag
     7381 agcaagaagt cttgccagtc ggattaactt tcgacgacgt cttgttgatt cccgccgaat
     7441 ccgcagtctt gccgcgagac accgatgtct ccacccgtct aacagaacgc attaccctga
     7501 acatccccct cgtcagcgcc gcaatggaca ccgtgacgga atccaagaca gccatcgcga
     7561 tggcgcagga aggcggcatg ggaattatcc atcgaaatct tccggtcggt atccaagcag
     7621 gagaggtgga aaaggttaaa aaattcgaaa gcggcatgat ctccgatccg atcactgtgg
     7681 cgccggacca gcagatttcc gaagccaagg tgatcatgga caagtatggc atttcgggat
     7741 tgccggtgaa gcgcgacggc cggctggtcg ggattctgac gaatcgtgat ctgcgctttg
     7801 aaaagaagct cgaccgcacg gtttccgaag tgatgaccaa agataaattg gttaccgtga
     7861 aagagggggt cgatcttgat gaagcaaaag aacttttgca ccggcatcgg atcgaaaagc
     7921 ttttggtagt tgacgacaac ttcaatctca aaggactaat taccgttaag gatatcgaga
     7981 aacggaccca gtttcccagc gcttgcaaag acacgcgcgg ccgcctgcgg gtcggagcgg
     8041 cggtcggagt aggagcggac tgggaggagc gagtcgaagc cctggtgcgc gcgggcgtgg
     8101 atctcatcgc catcgatacg gcgcacgggc attcaaaaaa tgtcctggat accatccgac
     8161 aaattcgggg acgctacccg gaccttgatc tttccggcgg caatgtcgct acagccgaag
     8221 ggaccacggc tctgattcag gcgggtgtca atgtcgtcaa agtcggcgta ggtccggggt
     8281 cgatttgcac aacccgggtg gtttccggag tcggagtgcc gcaagtgacc gccattggaa
     8341 gttgcgccaa ggccgcggcg cgccataacg tgccgattgt cgccgatgga ggtatcaaat
     8401 tctccggcga catcaccaaa gctttggctg ccggagcgga cagcgtcatg atcggcagcc
     8461 tatttgccgg aaccgaagaa agtcctggag aaacgattct ctatcaggga cgaacctaca
     8521 aagtctatcg tggtatgggt tcgttggggg caatgaagca gggcagcaaa gatcgctata
     8581 gccaaggcga tgttgaagaa gccatgaagc tagtgcccga aggcatcgag ggacaggtgc
     8641 cgtacaaagg ttcgttgtca ttcaatattc atcagttggt tggaggcttg cgcgcgggta
     8701 tgggctatct gggttgccga gatgtcgcca cgcttcgggc taaagccagg tttatgcaga
     8761 tcaccgcggc gggattgaga gaaggccatg ttcatgatgt cttcatcacc aaagaagccc
     8821 ctaactacag cgtcgaatga tcctcgttct tgattttggt tcccaataca cacaactcat
     8881 cgcgcgccgc attcgcgaat cccacgtgta ctgtgagatc catcctttca gtgtcggcct
     8941 cgataaaatt gcggaactaa atcctcaggg aatcattctt tccggcggac cggccagcgt
     9001 ctaccaggaa aacgcaccca aagttaagcc tgagcttttc gatcaaaccg tgccatttct
     9061 aggtatttgc tacggtatgg gagtgatcaa tctcgcctcc ggtggcgagg tggcgcgcgc
     9121 tgatgatcgt gagtacggac ccgctgatct gatgatcgac agcaacgccg acctgtttta
     9181 tgggtttaaa aaaaatgatc cggtgcgggt gtggatgagt catggggata agatgaccgt
     9241 tatccctccc ggttggagca gccttgccca cagcggcaat tcgcccatcg ccgccttcgt
     9301 cgaccccacc cggcagtttt ttggcgtgca gtttcatccc gaagtggcgc atacgccgcg
     9361 aggcaaggaa attctcgata attttgtgtt ccgtatctgt ggttgcaagc ccgactggac
     9421 gatggaacat tttatcgaag cttccgtaca gaagattcgc gatcaggtcg gcacgggacg
     9481 ggttttatgc gccctcagcg gcggggtcga ctcatcggtg gccgctagct tagtttatcg
     9541 ggcgatcaaa gatcggttgg tctgcgtttt cgtcaataat ggtttgttgc ggcagggaga
     9601 agccgagcgc gtttgccaac tattcagcga acgctttggc gagagctttc gatacgtgga
     9661 cgccagtgag gcttttcttt cggatttgag cggtattgaa gatccggaaa taaagcgcaa
     9721 gaaaatcggc tatagattca tcgaagtttt tgaaagcgag gccgagaaat taggcgaaat
     9781 cgattttctc gcccaaggaa cactctatcc ggatgtgatc gaatcggttt cgtttgtcgg
     9841 tccttcggtt actattaaaa gccatcacaa cgtcggtggt ttaccggctg caatgaaact
     9901 caagctcatc gaaccattgc gcgagctgtt caaggatgaa gttcgtattc taggcaaaga
     9961 gcttggattg cctcatgaaa tgattcaccg gcagccgttt cccggacctg gactggcgat
    10021 ccgcattctg ggccccgtaa cggaagagcg gctccggatt attcgcgctg ccgataccat
    10081 cgtctggcat gaaatgcgcg ccgcccggct gtatgaaaaa gtctggcagt cttttgcggt
    10141 tttgttgccg gtaaaaactg tcggagtcat gggtgacgag cgcagctatg aaaatgtcat
    10201 tgctctgcga gtggtcgaaa gtcttgacgg catgactgcc aactgggttc ctttgcccgc
    10261 tgatttgcta tcaagaattt ccaatcggat catcaatgaa gttcgtggag tgaaccgcgt
    10321 tgtctatgat atctcttcaa aacctccggc cactatcgaa tgggaatagc aactcattgc
    10381 gcatggagtt gagcgaccgg tgtccgttcg ttgatgaaag agatttcaca ggttctactg
    10441 ttcgacagcg accgccgctt attggtctac cttcgcgaca ataacccggc cattccgttc
    10501 ccaaatcact gggatttctt tggcggccat ctggaagcag gggagactcc cgaacgggcg
    10561 ttggtgcggg aagtaaaaga agagctcgga gttgaattaa agcagtggaa attttttcgg
    10621 ctttatcatt gcacccgagg agacgcttat cccaatctta aacacattta ttgggcgaag
    10681 atcgatcaat ccgccgcgca gctgacgctt aacgaaggac agaggctgat gagtattgga
    10741 ctcgaagaac gagatcgcgt aaagtttgcg aatatcctcg cgcaaatcct cgaagacttt
    10801 atcggaagcg ggctctggcc ggaacgcgga ctccattcgt gaaacgaaat tgcgtgatcg
    10861 aattgtcggg tccatgccgt ttgtgctaag tcatgattac cgcaatggaa cactctcgtt
    10921 ttgtacatct tcactgtcac accgaataca gtttgctgga tggcgccaac aaggtcgaga
    10981 aactattcaa gcgcataaaa acgctcaagc agccggccgt ggccatgacc gatcacggca
    11041 acatgtttgg cgcggtcgag ttttatcgtg aggcgattag ccacggtatc aagccgatca
    11101 tcggctgcga aatttatgtc gcgccgacta gccggttcga gaaaaaaggc gtcgatcgcg
    11161 gacccaagga gtacaacaat catcttattc tgctggcgat gaacggtgag gggtatcgta
    11221 atctgtgcaa gttggtttct ctcggctaca tggaaggatt ttattacaag ccgcgcatag
    11281 ataaggaatt gctcggtgag cttaacggcg gattgatcgc gctcagcgct tgcctgcaag
    11341 gcgaagtctc ccaggcgctg agctatggca acgttgagaa agccaaaacc gcagcggcga
    11401 gctatttatc gatcttcggt gatcgctact acatagaagt ccaagacaat aaactggcgg
    11461 aacaggagaa ggtcaatcga ttgctggtcg aaatggctaa ggacttatcg atcccggtgg
    11521 tggccactaa cgattgccac tatggcgagc gggatgattt tcacgcgcat gatgttcttc
    11581 tttgcgtgca gaccggcaag accgtgaccg acgataatcg gctcaagctg gacaccgacg
    11641 aactttattt aaaatcagcg gaagaaatga ttggcggctt cgactattgt ccgggggccg
    11701 tagagcgaac tctggagatc gctgaacgct gtaatgtcga aatggagttc ggcaaatatc
    11761 attttccgaa ctatacccct cctaaagaga tcagcctaga cgactatctc gacgagctcg
    11821 cccgtagcgg tctcgaagag aggcttgccg gtgtcgtcga gcctgggctc agaagcagct
    11881 acgtagaacg acttgagtac gagctcgaga ttatcaagag aatgcaattt cccggctatt
    11941 ttctgatcgt ggcggatttt atcaactacg ccaaacagaa cggcatcccg gtgggaccgg
    12001 gacgcggctc atccgccggc agtttggtgg cgtacgcgct caaaattacc gacttggatc
    12061 cgattcggca tgttttgctg ttcgagcgat ttctcaatcc cgagcgccgc tcgatgccgg
    12121 atatcgatgt agatttttgc atccgcggca gggcacaggt gattcagtac gtcaaggaaa
    12181 agtacggcgc ggaccgggtc gcgcagatcg cgactttcgg aactttgaag gccaaggcgg
    12241 ccatcaaaga tgtcggtcgc gcgctgggat ttaccttcgc tgaaaccgat atgattgcga
    12301 aactgattcc cgcgccgaaa caaggatttg attatccact gac
//
LOCUS       DASUVS010000052        18270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_18722, whole genome shotgun sequence.
ACCESSION   DASUVS010000052 DASUVS010000000
VERSION     DASUVS010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 18270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18270
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18722"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="VF182_02920"
     CDS             complement(<1..1423)
                     /locus_tag="VF182_02920"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6436056.1"
                     /translation="MAKREQKATESAGNKPIQDLRQWVERVEEMGDLVRVSEPVSCDE
                     EMSAIGYLVAKQKPSPAIIFDNIKGYESSPFKARSLWNILGPSLQRIALTMEEPPDTP
                     TVELIRRVKDKLKHRTPPREVQQSEAGFFQNTLMGDKIDLTQLPIPKHWPLDGGRYAG
                     TADCVITRDPDTGYLNVGTYRMMLQGKKEAGLYLSPGKDARLHITRSWQMGKPVEVAA
                     AWGIDPLFMVVGSQTFAKNVSEYEFLGGIKGEPIPVVKGKTTDLLLPANAEFMVEGVI
                     KPNSVKSEGPFGEFPGYYGRPEAGCPLVEITAIHYRSNPILTNALMADYPSNEQSGFF
                     AIIRSARIWDDLDKLGVPGIQGVYAHPAGAGGFGMTVISLEQRYAGHAAQALALAAQV
                     PGGAYYTKWIIAVDEDIDPTDMDQVIWAMSSRCNPIDDVDILRNTWSTWLDPTQNPPE
                     KRPYGSKALINACKEHRYLPVFSK"
     gene            complement(1472..2221)
                     /locus_tag="VF182_02925"
     CDS             complement(1472..2221)
                     /locus_tag="VF182_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6436057.1"
                     /translation="MSKSSRKELEELKTKVALGNRIMFHQGLADYHGHVSARIPGTRK
                     FFIKPVLAPLGEISSRDIIMVDIDQYMEICEENYAKAGNQRAITKLKNPPRETMIHAS
                     IYAARADVNSVVHTHQSLATAFSVAGTPILPIYNQAAVFAPETPIFPSPRLIYTIQDG
                     KEVCQTLGNRMAMLLKGHGVIVCGDSLEYATVHAIYLERTAYMQFVASCVGKPSIMPQ
                     KDIDYMKENMTYRAYDGFAYFKSLLPKKGRL"
     gene            2987..3367
                     /locus_tag="VF182_02930"
     CDS             2987..3367
                     /locus_tag="VF182_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436058.1"
                     /translation="MRMMLPPLKDRRTVDRFLSSFFSEYKPLHFKKAISIVCRFYNLK
                     NPRIEWFEYLDWGKTAGKTYEDGQIYLVHPESWKRGRKYYSERRWINTVYHEMGHYVL
                     WADAETKADKFALQMVRGLNHQKS"
     gene            3408..6236
                     /gene="ileS"
                     /locus_tag="VF182_02935"
     CDS             3408..6236
                     /gene="ileS"
                     /locus_tag="VF182_02935"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX6436059.1"
                     /translation="MDYKDTLNLPKTSFPMRANLPQNEPKLVEKWRNDHLYFEILKAN
                     EGQPKFILHDGPPYANGNIHIGHALNKVLKDIIVKYKAMTGHLAPYVPGWDCHGLPIE
                     LQVEKNIGRAKKLAMSKAEVRRLCREYAAKYVSIQREEFRRLGVLADWQEPYLTMAPS
                     YEAQEIRELGKFVASGTLYRKKKPVYWCASCITALAEAEVEYEEHVSSSIYVKFFVKD
                     SKGKFNADDFPTYFAIWTTTPWTLPANQAIAVNPKFIYRRVRTRAGDLIIDEELVPTV
                     MTAIGLEAASYVITEGSWIGAELEGIVCRHPWLDRDSRIVLGEFVTRDQGTGCVHIAP
                     GHGQEDYEVGMRYGLEVMAPVDAEGRFTAEAGDLEGESVFRVDPKIIQKLRDHGALLK
                     EETLPHSYPHCWRCKKPVIFRATEQWFISMDKNGLRDAALKAIEQVQWVPPWGKDRIR
                     GMLESRPDWCISRQRSWGVPIPAVYCKNCNEAILTQDLCEHIAAIVDVEGSDAWFIRP
                     ITELVPAGLACPGCGKNDFFREEDILDVWFDSGVSHAAVVEIDPRLGGRANLYLEGSD
                     QHRGWFHTALLTSLATRGRAPYESVLTHGFTLDGRGRKMSKSLGNVIAPQEITKKSGA
                     EILRLWVAAEDYREDVRISDEILNRLVESYRRLRNTARFLISNLYDFDPEHDIVLPPD
                     LDELDRWILHRTESLLSRCRDAYENYEFHTVFHGLNNFCSVDLSAQYLDIVKDRLYCT
                     GAKTTERRAAQTTLHRILNVLVHLMAPILSFTAEEIWTYMGKNHMSGSIFLSQMPQPD
                     TGMMDRELAGRWDRIFKERAEVLKALEEARTKGIIGHSLDAKIVLSNQNGGPASSLGD
                     LIKSDKSKVQDILIVSQADTAKSIASDRKGLSRYRAELMNCEIAVSKAEGLKCERCWK
                     YDTEVGRDVSHPTVCARCAAVLNSGVAA"
     gene            6233..6730
                     /gene="lspA"
                     /locus_tag="VF182_02940"
     CDS             6233..6730
                     /gene="lspA"
                     /locus_tag="VF182_02940"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX6436060.1"
                     /translation="MSRKARIVSGCLGSIVIADQLTKYVVDRTMPLHYSIPIIDGFFN
                     LTYIRNTGAAFGIFSGSHEVFRLPFLITVSLLAIGFIFMMLKRLRETETGLITALAFI
                     LGGAIGNLIDRIVHGEVVDFLDVYWSDYHWPAFNLADSFITIGVGITIYYLAIVKGDD
                     PFAAK"
     gene            6884..7645
                     /locus_tag="VF182_02945"
     CDS             6884..7645
                     /locus_tag="VF182_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436061.1"
                     /translation="MPSSTSWQATPFKRANTAAQVAAELPTLSLPAPGGRIIFIGVHG
                     GNPPTWEDAEWFDPQQWLPYRVIDLQNQSAVTEIAYHESVMHVTALQIGRYFPNSVTI
                     MIRGDSQVVSDLLDAIYHVGDRILLAGHSFGGRVVEELARDLKRREIVVDALVYIESF
                     WSNGVVPSNVKRALNFYVPAAFTFCRGLEKIKAEDSIGTEAINIAIPNPRGPYGGLCA
                     EHRNIDSDPRVWEGIFDRVITKIDFRNPYSALPPS"
     gene            complement(7596..7919)
                     /locus_tag="VF182_02950"
     CDS             complement(7596..7919)
                     /locus_tag="VF182_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX6436062.1"
                     /translation="MPEPNPPFDEPVVFPVGDILDLHAFQPKDIASAVEEFLDVCRRS
                     GILQARLIHGKGAGVQRAIIRSLLDKHPAVISFQDAPAEAGGWGATIVSLVAVQSTDS
                     GNRSL"
     gene            complement(8020..10455)
                     /locus_tag="VF182_02955"
     CDS             complement(8020..10455)
                     /locus_tag="VF182_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6436063.1"
                     /translation="MHENFAKPLSPVFGPPPPAPVLFGRSGSALYWLTITLLVCGVFF
                     ADLYSPFGVAIPTSYVAALLLVLALPGAREKVLLALLSTALIILDYFLTPSSPSIPGW
                     IPIFNDVLTVTLIWIIAALGLRHRRAQDAMRRSEERFRALVEASSQIVWTADGAGNII
                     EDSPSWRAFTGQTFEQRRGSGWLNAYHPNDQKRVHMQWEDATARKTPLEGHYRLRRHD
                     GQWRWTSVRAVPLLSADGSARGWVGMNTDITERKQAEADTLFLLDLSDCIRLASDPDE
                     LTWAVAVALGEYLQTERSSFIEADPGGGRFSIHRDYHLNLPSLAGTYRLSSLGTKIVS
                     DSQAGHNTVVSDTSRDDRTAIYGEYYRRIGIGAFAATPLLREDRLVSALFVSTAEPHD
                     WSEREVSLIAAAAERTWLAVERLRLNSALTQSEEALRDADRRKDEFLATLAHELRNPL
                     ALVRNVVSLSQASGFSEAQQRWGRDVIDKQVGYLTRLTDDLFDISRITREKLGLHKEQ
                     VELCEIVKTAVDSSRPVIEERRHKITITLPESPVYVEADRVRLTQVVMNLINNAAKYT
                     PDPGQISLTLGLQNDHAVLQVKDTGIGIAPEDLPRLFDLFYQADRSFTRAEGGLGLGL
                     TLVQRILELHGGTVHAFSSGAKQGSEFVVSLPILQNAFQQNSSQDVDHELFATKSRVR
                     RILVADDFPQAAETLGGLLRQEGYEVQIAQDGLEAFQAAERSHPDVIVLDLAMPELDG
                     YDAARKIREQPWGKQVVLIALTGWGQERDRHRTKEAGFDAHLTKPVKYEAILELLGNL
                     ANRQRPGSAAPNV"
     gene            10733..12328
                     /locus_tag="VF182_02960"
     CDS             10733..12328
                     /locus_tag="VF182_02960"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6436064.1"
                     /translation="MARRKYYRDFREHLQELEDREKLIRIRRRIDKDTELMPLVRWQF
                     RGLDEEQRKAFLFENVVDVKGKHYSMPVTVGTLAASTEIYAIGLMCRPEDIHQRWTQA
                     QLSPVEPVKVASGPSQEMVWTGNELLNGHGLDMLPVPISTPGFDNAPYLTSANWITKD
                     IDTGVYNIGNYRGQIKSPDRAGGLFTSQHMGQHWRKCKARGIPLQAAIAIGVIPSIAY
                     AATAKIPYEFDEYRLAGGLAAEAVEVVQCKTVDLLAPSTAEIIIEGTIPTDSLEPEGP
                     FGEYPGYMGHRTNSPYLEVTCITHRRDAIYTALMSQFPPSESSKIKHTGTEKILYKYL
                     RHDSGNAAVLDVALHDEVSGSGQAYCVIRMRNASKAEAWRALNASAGFTGSYAKVCIA
                     VDEDINIHDPAMVNWAICFNVRPDEDILIAKGKSPGLDPSAHRPGMESLEVRMASIGA
                     LLINAVRPWPYTPVSLPRKEFMDRSRSIWDELGLPPLKPRMPWYGYSLGAWTKEEEEE
                     AELAIKGEYFTTGVKQKGQRTKP"
     gene            12430..13185
                     /locus_tag="VF182_02965"
     CDS             12430..13185
                     /locus_tag="VF182_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6436065.1"
                     /translation="MNLDPTLILVGTTLLFAAFVKGASGLGFPLIATPMVALLLDIRI
                     AITILLIPNIVMDIAQVFRDGFPFAVLRRFVWFFLATIAGVFLGTAVLVNLPLWILNF
                     CLGTMILVFVAFNLLKFEFGITPSLENRLSLPVGFISGFLNGLTNAAGPVVAIYLYSL
                     KLGKREFVKSIATIFVFTKMSQLAAVSSWNLFNGSTLMLSLQVTLFVLAGFYAGIKTQ
                     DRINQQTFNRALLIVLFVIGVTLLVRALTQSSS"
     gene            complement(13236..13955)
                     /locus_tag="VF182_02970"
     CDS             complement(13236..13955)
                     /locus_tag="VF182_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007458298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX6436066.1"
                     /translation="MASWDDILTVRDKEVFSMSGYGKRAGFGQRPAVLVIDVNYNFVG
                     DKPEPILDSIKRFRNSCGTEGWDGVHRISELLAECRKKHIPTFYTTGHDDRTNAVAFG
                     RWQAKNSRSTEDMTAIWEKGNDIVEEIAPRGGDIVVRKQKPSAFFGTPLMSMLNEVHA
                     DSVLVAGTTTSGCVRASVIDAFSYNLKVSVIEECVFDRGQASHKINLFDMAMKYADVI
                     SLQETLEYVRGLPDNLFAPAL"
     gene            14188..14664
                     /locus_tag="VF182_02975"
     CDS             14188..14664
                     /locus_tag="VF182_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6436067.1"
                     /translation="MWTDGEVPFVPTPVDVVDRMLEMARVTPADVVYDLGSGDGAIII
                     RAAQKFGVRGVGIEIDADLVRKAQANAYKNKVEHLVEFRVQDALSVDVSEATVVTLYM
                     LPEFNAKLRPILDRQLKPGARVISHDFPIEGWVADRTERVSGDFLHDHTILLFEMR"
     gene            complement(14849..15205)
                     /locus_tag="VF182_02980"
     CDS             complement(14849..15205)
                     /locus_tag="VF182_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEX6436068.1"
                     /translation="MHTGNQSLGRTRILLLAGAMFVTGCAGGSLSTREKGAGVGALGG
                     AAAGGLIGAAVGHPGAGAAIGGAFGLGAGALVGDQLQGQEITNSQQQQQIQRNQAEIT
                     RNRQTIQRLERQRNEY"
     gene            complement(15551..15718)
                     /locus_tag="VF182_02985"
     CDS             complement(15551..15718)
                     /locus_tag="VF182_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436069.1"
                     /translation="MQTDHFVEVMALLNAIVRCFNDNVGKIDMNKLAQPAFAAPAIIA
                     VTMNRMEAKKA"
     gene            complement(16013..17368)
                     /locus_tag="VF182_02990"
     CDS             complement(16013..17368)
                     /locus_tag="VF182_02990"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6436070.1"
                     /translation="MAHPRAVVLVVDDDSATRQGLTALLQSWGYATLDAADAAAALKI
                     CDKEAPHAVITDLMMPGTSGLQFIESLGEQVRQMAVVFVTGQATVDTAVQAIKLGAYD
                     YLPKPLEPPRLRITLEKALKQVAFAREAAALRLKLESPLGSYGALIGKSAPMRQLYQQ
                     ISKIAGTDASVLITGESGTGKELVAQTLHELSPRREATFLPLNCAAISATLMESELFG
                     HEKGAFTNAIERHPGCFEQADDGTLFLDEITEMPLEVQAKFLRVLEERQVRRIGGNAN
                     IPVSVRVVAATNREPASAVKEGKLRQDLFYRLSVFHLEMPPLRQRGDDIPLLGHYFLE
                     FFATKYHRPVPEWAGDYTAALQSHSWPGNVRELRNAMERLSVLSPGPTLTARDFEQFC
                     LKAVVEEKSELTPMSLAEAERQAIEKALAASEGNKTQAARLLGITPKTLSVKLALYGK
                     A"
     gene            complement(17371..>18270)
                     /locus_tag="VF182_02995"
     CDS             complement(17371..>18270)
                     /locus_tag="VF182_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6436071.1"
                     /translation="LLFALGGGIALVSAFLITALYTRRFRIISETLSQAEAGTYAARP
                     PYANEDEVGRSLDLIDRLVMKQRNTVAAPGTAQRLALAARTLAHEVRTPLNALAIHLE
                     LLRNPAQSTDDSGAAVDQQHRSIAALDSGLRQVNRLIRDFTDYSAPVTMEHKPVDVAE
                     VLRTSLEAIGSVCAAQKISIAQARLQGPWQVHGDATRIRQAFDNLLRNAVEAQPDGGA
                     IRITAEKDDSRLIVDINDAGPGVPPEQQAEIFDFGKTTKPSGNGIGLPLSQLIVEAHG
                     GSLTYHSNGHGATFRLILPLESN"
BASE COUNT         4273 a         4724 c         4800 g         4473 t
ORIGIN      
        1 gttttgaaaa gaccggtaga taacggtgct ctttgcaagc attaatcagg gccttcgatc
       61 cgtaagggcg tttctccggt ggattttgag ttggatcgag ccaggtgctc caagtgttgc
      121 ggagaatatc tacgtcatcg atcggattgc agcggctact catcgcccag atcacttgat
      181 ccatatccgt cggatcgatg tcttcatcga ccgcgataat ccatttcgtg taataagcgc
      241 ctccaggtac ttgggcagcc aacgctaagg cttgtgctgc atggccggca tatcgctgtt
      301 cgagactgat taccgtcatg ccgaaacccc cggcgccagc cggatgcgca tagactccct
      361 gaatgcccgg cactccgagt ttgtctaaat cgtcccagat gcgcgccgat cggatgatag
      421 caaaaaaccc actctgctcg ttggacggat aatcggccat taaagcattg gtgagtatcg
      481 gattgctgcg gtaatggata gcggtaattt ccacaagagg gcagccggct tcgggtctgc
      541 cgtaatagcc cggaaattca ccgaatgggc cttcgctctt caccgaatta ggcttgatta
      601 cgccttcaac cataaactca gcatttgccg gcagcagcag atcggttgtc ttacccttga
      661 ccacagggat cggttcgccc ttgatgccgc ctagaaattc atattccgaa acgttcttgg
      721 cgaaggtttg cgatccgaca accataaata gtggatcaat tccccatgcg gcggcgactt
      781 caaccggttt gcccatctgc caagaacggg tgatatgcag gcgcgcatct tttccaggag
      841 aaaggtagag gccggcctct ttcttacctt gaagcatcat gcgataggtg ccgacgttta
      901 gatagccggt atcgggatcc cgggtgatga cgcaatcggc cgttcctgca tagcgaccac
      961 catccaacgg ccaatgcttg gggatcggca gctgagttaa atcgattttg tcacccatta
     1021 aagtgttctg gaaaaagccg gcttcggact gctgcacttc ccggggtggt gtccggtgtt
     1081 tgagcttatc ctttacccgt ctgattaatt ccaccgtagg ggtatcagga ggctcttcca
     1141 ttgtcagcgc gatccgctgc aaacttggtc ctaagatgtt ccagagagag cgcgctttga
     1201 acgggctgct ctcatagccc ttgatgttgt cgaaaatgat tgccggagaa ggtttttgct
     1261 tggcgaccaa gtagccgatg gcgctcatct cttcgtcgca gctaacaggt tcgcttacgc
     1321 gtaccaggtc gcccatttct tcaacccgtt cgacccattg acgcaaatcc tgtattggtt
     1381 tgttccctgc tgattctgtg gctttctgtt cacgtttggc catcgctatt aacctccagt
     1441 gcttttctct cagactaaga aatacgagct gttaaagccg gccttttttc ggcaacagag
     1501 acttaaaata agcgaagcca tcataagcac gataggtcat attttctttc atgtaatcga
     1561 tatccttctg cggcatgatc gatggcttgc cgacgcagct cgcgacaaac tgcatatagg
     1621 cagtacgctc caaatatatc gcgtgaactg tagcatattc caagctgtcg ccgcacacaa
     1681 taacgccatg tcctttgagt aacatcgcca tgcgattgcc cagcgtctgg caaacttctt
     1741 taccgtcttg gatcgtatag ataagccggg gactggggaa tatcggagtt tctggggcga
     1801 aaaccgctgc ttgattgtaa atcggcagga tcggggtgcc cgcaaccgaa aatgccgtcg
     1861 ccagcgattg atgagtatga acgaccgaat taacatccgc gcgagcggca taaatagaag
     1921 catggatcat ggtttcgcga ggcgggttct tgagtttcgt gatcgcacgc tggttcccag
     1981 ctttcgcgta gttctcctcg caaatttcca tgtactggtc gatgtcgacc ataatgatat
     2041 ctctgctcga gatctcgccg agcggcgcca gaacgggttt gataaaaaac tttcgggttc
     2101 cgggaatgcg agcgctgacg tgaccatggt agtctgccaa accctgatga aacataatgc
     2161 ggttgcctaa agctactttg gtttttaatt cttcgagttc tttcctgctg ctcttactca
     2221 tgactctcct tttgatacca gtgattctcg acaatgacgg agaattgcag ttgccgtcac
     2281 tctaacctga agatcagaaa acgcaaatgc gtcgaggtcc cattaagcgc cgtgctacgg
     2341 ataaaggata ggctgcgttt ctctacgttc attccagccc tcacctcaac ttcaggcgat
     2401 ccctaaattc actttcaacg aaatcttgtt caacggcaat catagcgcag cgctgccatc
     2461 cccatccgca cggtgaagtc ttgagccaga gattgtcgtg ccagtgatca aaactgataa
     2521 gcaaactcct tccaactggc ggaaatccaa ttgcggttat tgaatgagca tcggccgtca
     2581 taaatggcaa agcctttaag agccgatcgg tgcaactggt gataatagaa ccgaaaatcc
     2641 ccaagaagga tcacccattt gctagctgtt ccgacaaacg gtataagcac caacccgagc
     2701 ccgtcgggac cgcttgagtg acggatctta cttaaataag ggagctatgc gcttattaat
     2761 ggaggcgcga atagaccaaa gcaggccaac ttacgacagt tcgtcagagt cgcaagagca
     2821 tcatcaaagc ggccgatctc ggcatagctt cggatcgagg gactataata ttgaaaaata
     2881 tcaaccactt acagatttcg aacaattgag ctatttagcc cgccctattt tgatcgccat
     2941 ttttttgtgt tagaaaccta tcactaagtg gccgaggata tgtaccatgc ggatgatgtt
     3001 gcccccgttg aaagaccgtc gaactgtaga ccgtttcctt tcatccttct tcagcgaata
     3061 caagccactg cacttcaaaa aggctatttc gatcgtttgt cgcttttaca acctcaaaaa
     3121 tcctcgaatt gaatggttcg agtatcttga ttggggcaaa actgcgggaa agacgtacga
     3181 ggacgggcag atttatctag tccatcctga aagttggaag cgcggccgaa aatactacag
     3241 cgagcgtcgc tggatcaata ccgtttacca tgagatgggt cattacgtac tttgggcaga
     3301 tgcagaaacg aaggctgata agttcgcgct ccaaatggtc agaggcttaa atcaccagaa
     3361 gtcataatca ctgcgacctg ccgattcttt tcaataacaa ggtaacaatg gactacaaag
     3421 acaccctcaa tttgcccaaa accagttttc cgatgcgggc aaatctcccg cagaatgagc
     3481 ccaaactggt cgaaaaatgg cgaaacgatc atctctattt tgaaatcttg aaggcaaacg
     3541 agggtcaacc gaaattcata ctccatgacg gacctcctta cgcgaacggc aacattcata
     3601 tcggccatgc tctcaacaaa gttcttaaag acatcatcgt taagtacaaa gctatgaccg
     3661 gtcacttggc gccttatgta ccaggttggg actgccatgg tttgccaatc gaactgcaag
     3721 ttgaaaaaaa catcggcaga gcaaaaaagc tcgctatgag caaggccgaa gttcggcggt
     3781 tgtgtcgaga gtacgccgcg aagtacgtct caatccagag agaagaattc agaaggctag
     3841 gagttctagc tgactggcaa gagccgtatc ttactatggc accgtcctat gaagcccaag
     3901 agatccggga attaggtaaa tttgtcgcgt caggcactct gtatcgtaaa aagaaaccgg
     3961 tctactggtg cgcctcctgt atcaccgcgc tcgcggaagc ggaagtcgaa tacgaggagc
     4021 atgtctcttc ttccatctat gtcaaattct tcgtgaaaga ttccaaggga aagtttaacg
     4081 cggacgattt cccaacttac tttgccattt ggacgacgac cccgtggact ctgccggcga
     4141 atcaggcaat cgctgttaat ccgaaattta tctaccgccg ggtaagaacc cgcgcaggag
     4201 acttgatcat tgatgaagaa ttggttccaa ccgtgatgac ggcgattggt ttagaggctg
     4261 ccagctatgt aatcaccgag ggttcgtgga tcggcgcgga gcttgaaggc attgtttgcc
     4321 gtcacccctg gttggatcgt gattcccgga ttgtccttgg cgagtttgtc acgcgcgatc
     4381 aagggaccgg ctgcgttcac atcgcacccg gacatggaca ggaggactat gaagtcggaa
     4441 tgcgttatgg ccttgaggtg atggcgccgg tggatgctga aggtagattt acagccgaag
     4501 ccggtgatct cgagggagaa tcggtcttta gagttgaccc caaaatcatt caaaagttgc
     4561 gtgatcatgg cgcactcctc aaagaggaga ctctgcctca cagttatccg cattgttggc
     4621 gttgtaaaaa acccgttatc tttcgagcca ccgagcagtg gtttatctcg atggataaga
     4681 atgggctcag ggatgcagct ctaaaggcca tcgaacaggt gcagtgggtg ccgccatggg
     4741 gcaaagatcg tattcgaggc atgctcgaaa gtagacccga ctggtgtatt tcgcgccagc
     4801 gctcctgggg agttcccatt ccagcggtgt attgcaagaa ttgcaatgag gctattctca
     4861 cgcaagatct ttgcgaacac atcgctgcta tcgttgatgt ggagggttcc gatgcgtggt
     4921 ttatccggcc catcactgaa ctcgttcccg ccggccttgc ctgtccaggg tgcggcaaaa
     4981 acgatttttt tagagaagaa gatatccttg atgtatggtt tgactcggga gtaagccatg
     5041 cggcagtggt cgaaatcgac ccgcggctcg gcggtcgcgc caatctttat ctggagggaa
     5101 gcgaccaaca tcgcggttgg tttcacacgg ctttgctgac ttccttggcc acccgagggc
     5161 gcgcgccata tgagagcgtg ctcacccatg gcttcaccct agatggcagg ggtcgcaaga
     5221 tgtcgaagtc tttaggcaat gtcattgcgc cgcaggaaat caccaagaaa tcaggcgccg
     5281 aaattttgcg gctctgggtg gcagctgaag attaccgcga ggacgtgcgc atctcagacg
     5341 aaattctcaa tcgactagtg gagtcttacc ggcgtcttag aaacacagca cgttttttga
     5401 tcagcaacct ttacgatttc gatcccgagc acgatatcgt tttgccgccc gacctcgatg
     5461 aacttgatcg ctggatcctg caccggaccg aaagcttgtt aagtcgttgc cgggatgctt
     5521 acgagaacta tgaatttcat actgtgttcc atggtcttaa taacttttgt agcgtcgatc
     5581 ttagcgcgca gtatctcgat attgtcaaag accgtcttta ctgcaccggt gcaaaaacca
     5641 cggagcggcg agcggcccag acgacgcttc atagaatcct gaacgtgctg gtccatctaa
     5701 tggcgcccat tctatcgttc accgcggaag agatttggac atacatgggg aaaaatcata
     5761 tgtccgggag tatttttctc tcgcaaatgc cgcagccgga taccggtatg atggatcgcg
     5821 aacttgctgg cagatgggat cgtattttta aggagcgcgc tgaggtgctc aaggctttgg
     5881 aagaggcgcg aaccaaaggc atcatcggtc attcgctgga tgcaaaaatc gttttgagca
     5941 atcagaacgg cggcccggct tccagccttg gagatctgat caagtccgat aagagcaaag
     6001 tgcaggatat tctaatcgtg tctcaggcgg ataccgccaa atcgatagca tctgatcgca
     6061 agggtttgtc caggtacaga gcggaactaa tgaattgcga aatcgctgtc tcgaaggccg
     6121 agggtctcaa atgcgagcgt tgctggaagt acgacactga ggtcgggcga gatgtcagcc
     6181 atccgaccgt ctgcgcccgt tgtgccgcag ttctgaattc cggagtcgcg gcttgagtcg
     6241 caaagctagg attgtgtccg gctgcctggg atctatcgtc attgccgatc aactaacgaa
     6301 gtatgttgtc gatcggacga tgccgctaca ttactcaatc ccgatcattg atggtttctt
     6361 caaccttacg tacatccgca atacaggtgc tgcattcggt atcttctctg gcagtcatga
     6421 agtttttcgg ttgccgttct tgatcactgt ttcgctgtta gcaatcggct ttatttttat
     6481 gatgttgaag cgtctgcgcg aaacggagac tggtctgatt acagccctcg ccttcattct
     6541 cggcggcgcc atcggcaacc ttattgaccg gatcgttcat ggcgaggttg tcgatttctt
     6601 ggacgtatat tggtcggatt atcattggcc cgccttcaat ttagccgaca gctttataac
     6661 catcggtgtg ggtatcacga tctattatct cgccatagtt aagggcgatg acccgttcgc
     6721 cgcaaaataa ttttctcagt ggggaccgcg tggcttgctc ttagaactgg agctatggct
     6781 agcattgcct aacctgatgg gtctgcatct tagggacatc acactcctat cgctattgct
     6841 cctgcctatc gggacaataa gttgccaggc attgagtcaa actttgccat ccagcacaag
     6901 ctggcaagcg actccgttca agcgcgctaa cacagcggcg caagtcgccg ctgaacttcc
     6961 taccctcagt ctaccggcgc caggcgggag gattatcttt atcggtgtgc atggcggcaa
     7021 cccgccgaca tgggaagacg cggagtggtt tgatccgcag caatggttgc cctatcgggt
     7081 tatcgacctt caaaatcaga gtgcagttac ggagattgct tatcacgagt ccgtcatgca
     7141 tgttaccgca ttacagatag gacgatattt tccgaacagc gtcacgataa tgattcgcgg
     7201 cgatagtcag gtcgttagtg atcttctcga cgctatttac catgttgggg atcgtatact
     7261 actggctggc catagtttcg gtggacgggt ggtcgaagag ctagcgcggg atctaaaacg
     7321 ccgagaaatc gttgtcgacg cattggtcta catcgagagt ttttggtcca acggtgtggt
     7381 acccagcaac gtaaagcgcg cccttaattt ctacgtaccc gcagccttca cattttgtcg
     7441 aggtttggag aagatcaaag cagaggactc aatcggcaca gaagcaatta acatcgccat
     7501 accgaacccg cgtggaccct acggcggtct ctgcgccgaa caccgcaaca tcgacagtga
     7561 tccacgtgtg tgggagggta tctttgaccg cgttattaca aagatcgatt tccggaatcc
     7621 gtactctgca ctgccaccaa gctgacaata gtagcgcccc agcctccggc ttcggctgga
     7681 gcatcttgga aagaaatcac agcaggatgc ttatcgagaa gagatcggat gattgcgcgt
     7741 tgaacgcctg cgcccttccc gtgaatgaga cgcgcttgta gaattcctga ccggcggcat
     7801 acgtccaaga attcctcaac ggcgctggcg atgtccttgg gctggaaagc atgaagatca
     7861 agaatgtctc cgacaggaaa aaccaccggt tcgtcgaaag gagggttagg ctccggcata
     7921 gttcgactca gttccttctc gcgcagttta gatcgcgctt cgggttttca caataaattc
     7981 cccaccttct gcatgcgcat gccgtttcaa ggcaatattt caaacgttag gagcggctga
     8041 tcccggccgc tggcggttcg ccaaattgcc gagcaattct aaaattgctt cgtacttaac
     8101 cggtttcgtc aggtgggcgt caaacccggc ctccttggta cggtgccggt cccgttcttg
     8161 tccccacccg gtcagagcaa tcagcacaac ctgtttgccc cacggctgct cgcggatctt
     8221 tcgcgcggca tcgtagccat ccagttcagg catagccaga tcgagtacga tgacatctgg
     8281 atgtgagcgc tccgcggctt ggaaggcttc aaggccgtcc tgggcaattt gaacctcgta
     8341 tccttcctgt cgtagtaatc cgccgagagt ttcagcagcc tgcggaaagt catcggccac
     8401 taggatgcga cgaacccggc tctttgtggc aaaaagctca tgatcaacat cttggctcga
     8461 gttctgctga aacgcatttt gtagaatggg caaactgacg acaaactcac tgccttgttt
     8521 agctcctgag ctgaacgcgt gcaccgttcc gccgtgaagt tcaaggattc tctgtaccaa
     8581 ggttaaacca aggcccagac cgccttcggc gcgggtaaac gatcggtcag cttgatagaa
     8641 caaatcgaac aaacggggca agtcctcggg agcgattccg atcccggtgt ccttaacctg
     8701 caagacagca tgatcgttct gcagtccaag agttaacgaa atttggcccg gatcaggcgt
     8761 gtatttggcg gcattattga tcaagttcat gaccacttgg gtcaatcgaa cccggtccgc
     8821 ctctacgtag acaggtgatt caggcaaggt aattgtgatc ttgtgccggc gctcttcgat
     8881 tactggacgg cttgaatcta ccgctgtctt aacgatttca caaagctcca cttgttcttt
     8941 atgaaggccg agcttttctc gggtgatacg gctaatatcg aaaagatcat cggtgagccg
     9001 agtcagatat cccacttgct tatcgataac atccctgccc catcgctgtt gggcttcgga
     9061 aaaacccgaa gcctgagata agctgacaac gtttcgcacc agagctagcg gatttcttag
     9121 ttcgtgcgct aaggtcgcca ggaactcgtc cttgcgccga tcggcatcgc ggagcgcctc
     9181 ttcgctttga gttaatgcgc tgtttagccg caatctttct actgctagcc acgtgcgctc
     9241 ggccgcagca gcgataagcg agacctctcg ctcactccaa tcatgcggtt cagctgtaga
     9301 gacaaataac gctgacacca gcctgtcttc tcgcaataga ggggttgccg cgaacgctcc
     9361 gatccctatg cgccgataat attcgccata aatcgcggtt cggtcgtctc gcgaagtatc
     9421 agagacgacc gtattgtgac cggcctgaga atcgctgact atcttggtgc caagagagct
     9481 cagccgataa gttccagcca aagaaggaag attaagatga taatcgcgat gaatactgaa
     9541 tcgaccccct ccagggtcgg cctcgatgaa actggagcgc tcggtttgta aatattcgcc
     9601 aagtgcgacc gccaccgccc aagtcagctc atccggatcg gaggccaggc gaatgcaatc
     9661 actcaggtcg agcagaaaaa gcgtatctgc ctcggcctgt ttacgctcgg tgatatcggt
     9721 attcattccc acccagccac gcgctgatcc atcagccgac aagagcggaa cggctcgtac
     9781 ggaagtccat cgccactgtc catcgtgccg tcgaaggcgg tagtggcctt ccagaggtgt
     9841 ttttcgcgcg gtcgcgtctt cccattgcat atggactctc ttttgatcgt tcggatggta
     9901 cgcattgagc cagccggaac cgcgccgttg ctcgaaagtc tgcccggtaa atgcccgcca
     9961 ggaaggtgag tcttcgatga tattgccggc cccatcggcg gtccaaacaa tctgcgacga
    10021 ggcttctacc agagcacgaa accgttcctc actgcggcgc atggcatctt gcgcgcgcct
    10081 atgacgcaaa cccaaggcgg cgatgatcca gatcagggtc accgtgagca cgtcattgaa
    10141 aatggggatc cacccgggga ttgaaggtga actcggcgtt aaaaaatagt ccaggataat
    10201 gagcgccgta ctaagcaggg caagcagaac cttctcgcga gctcctggca aggccagcac
    10261 taacagtagc gcggctacat agctggtcgg tatggcaaca ccgaatggcg aatataggtc
    10321 ggcgaaaaaa acgccacata cgaggagagt gatcgtcagc caatatagcg ctgaaccaga
    10381 tctgccgaag agaactggcg ctgggggtgg tggcccgaac accggagaaa ggggttttgc
    10441 gaagttttcg tgcataagct gcagccgcta attgttcact ccgacaagct cagtagtgta
    10501 gcaaagacgt cctggatgca aaagcaaaat acgcctggca tttctgcgcc gcatcgacga
    10561 catcggatct tccatcagcc ggcccctgtc atggaggact ttagagcaat cgtaaagagg
    10621 tgatttgatt ccgaacttgg tcctttctgt tcattaaaaa cccgattccc gcatgcttga
    10681 caagcacatc gcgtgcgacg atacatgtca gaagcactct tcaggagaga tcatggcgcg
    10741 ccgaaaatat tatcgtgact tccgcgaaca tctacaggag ctcgaggatc gggaaaaact
    10801 aattcgtatc cgtcgcagga tcgataaaga taccgaattg atgccattgg tgcggtggca
    10861 atttcgcggc ctcgacgaag agcagcgcaa ggctttcctg tttgaaaacg tcgttgatgt
    10921 aaaaggcaag cattattcga tgccggtgac ggtgggaaca ctggccgcga gcaccgagat
    10981 ctatgccatc ggtttgatgt gccggcccga agacatacac cagcgctgga cacaggccca
    11041 gttgagtccg gtcgaaccgg tgaaagtcgc ttcaggccca tctcaggaaa tggtttggac
    11101 cggtaacgag ctgttgaatg gtcatgggtt ggatatgctc ccggtgccga tctctactcc
    11161 aggttttgat aatgcaccct atttgacttc agccaactgg atcaccaaag acattgatac
    11221 cggcgtttac aacatcggca actatcgagg tcaaatcaaa tctccggatc gcgccggcgg
    11281 gttgttcacg tcgcaacata tggggcaaca ctggcgcaag tgcaaagcca ggggtatacc
    11341 gttgcaggcc gcaattgcca tcggtgttat tcccagtatc gcctacgccg cgaccgctaa
    11401 gattccctac gaatttgacg aataccggct tgccggtggc ttggccgcgg aggccgttga
    11461 agtcgtgcaa tgtaagacgg tcgatctttt ggcgccgtct accgccgaga ttatcatcga
    11521 aggaaccatt ccaactgact ctctcgagcc tgagggaccg tttggcgagt accccggtta
    11581 catgggtcat cgaaccaatt ctccctatct ggaagtgact tgcatcacgc atcgtaggga
    11641 cgcaatctat acggcgttga tgagccaatt tccgcccagc gaatcgagca aaataaaaca
    11701 taccggaacc gagaaaatcc tatacaagta cctgcgtcat gatagcggca acgcagcagt
    11761 gcttgacgtg gcgctccacg atgaggtgag cggcagcggt caagcttact gtgtgatcag
    11821 gatgcgtaat gcctcgaaag cggaggcctg gcgtgcgctc aacgcatcgg ccgggtttac
    11881 gggcagctat gcgaaggtct gcattgcggt agatgaggac attaatattc atgatcctgc
    11941 catggtgaat tgggccatct gtttcaacgt tagaccggac gaagacattc ttattgcgaa
    12001 aggaaagtcg ccggggttgg atccttccgc tcatcgaccg gggatggaaa gtcttgaagt
    12061 acggatggca tcgatcggcg ccttgctgat caatgcagtg cgcccgtggc cctacacccc
    12121 ggtctctttg ccgcgcaagg aattcatgga caggtcgaga tccatttggg acgaattggg
    12181 tctcccccca ttgaagccgc ggatgccctg gtatggttac agtctcggtg cctggacaaa
    12241 agaggaagag gaagaagcgg aactggcaat caaaggtgaa tacttcacga caggcgttaa
    12301 gcagaaagga caaagaacca agccgtaacc tgcgacatca gccaacacat ttgagttgct
    12361 gtttgccgat cactgcccac catcttgagt gtttgacata gcgctatccc ggacaactct
    12421 cgatcaatca tgaaccttga tcctacgctg attcttgttg ggacgaccct gctgtttgcc
    12481 gctttcgtca aaggagcttc gggacttggc tttccattga ttgccacacc gatggtggct
    12541 ttgttgctgg atattcggat agccatcacc atcctgctaa ttcctaacat cgtcatggat
    12601 attgctcaag tattccgtga tgggtttcct tttgccgtat tgcgccgctt tgtttggttt
    12661 ttccttgcga ctatcgccgg agtgtttctg ggtaccgcag tcttggtgaa cttgccgcta
    12721 tggatattga atttctgcct cggtaccatg atcttggtat tcgtagcatt caacttgctg
    12781 aagttcgaat tcggaattac acctagtctg gaaaacaggc tttcgctgcc ggtaggattt
    12841 atcagcggct tccttaacgg tctcaccaat gccgccggtc cggtggtggc gatttatctc
    12901 tacagtctta agctgggtaa gcgcgaattt gtcaaatcga tagcgacgat cttcgtgttc
    12961 acaaagatga gccaattggc tgcggtctcg agctggaatt tgtttaacgg ctcaactttg
    13021 atgttgtcat tgcaggttac tcttttcgtt ctcgccggct tttatgcggg tataaaaacc
    13081 caagatcgaa tcaaccagca aaccttcaat cgagctctct tgattgtgtt gtttgttatc
    13141 ggcgtaaccc tgcttgtgcg ggcgctcact cagtcatcat catgagcggc ggcatcgcaa
    13201 tgtggcccgc agttctccga atcatcgggc cagatttata gcgctggagc aaaaagatta
    13261 tccggcaatc cccgaacgta ttcgagtgtt tcctgaagag agatcacatc cgcatatttc
    13321 atcgccatgt caaaaagatt aatcttgtgc gatgcctgac cacgatcgaa cacgcactct
    13381 tcaattaccg agactttcag attataagaa aaggcgtcga taacgctggc gcgaacgcag
    13441 ccgctggttg tcgtgccagc aactaggacg ctgtcggcgt gaacctcgtt tagcatgctc
    13501 atcaaaggtg tgccgaagaa ggcgctgggt ttttgtttgc gcacgacaat gtctcctcct
    13561 cgaggagcga tctcctcgac gatgtcgttg cccttctccc aaatagcggt catatcctcg
    13621 gtcgagcgac tattcttggc ctgccaacgg ccaaaggcaa ccgcattggt tcgatcatca
    13681 tggccggtcg tgtaaaaagt cggtatatgc ttctttctac attcggcaag cagctcgctg
    13741 atccgatgaa cgccatccca accttcggta ccgcaactgt tacgaaaccg tttgattgaa
    13801 tccaaaatcg gctctggctt atcgccaaca aagttataat taacgtcgat caccaaaacc
    13861 gcaggtcttt gtccgaaacc ggcacgttta ccatagccgg acatcgaaaa aacttctttg
    13921 tctctcaccg tcagtatatc gtcccagctt gccatttttt gccccccttg gtcagatact
    13981 ttacggtgtc agatcacaac aaacctttgc ctttgtcaac gactcttcat tccatgcgtg
    14041 ggaaagccat tgacccagcg atcaagtcta ctttataacc agcgagtcga ttcattggat
    14101 agaggggttc aattgcccaa gcgaatcttg ttttcaacag tgtcattcct tttcctactt
    14161 ttagtcctaa atgcttgcgc tatcccagtg tggaccgacg gcgaagtacc atttgtaccg
    14221 acgccggtgg acgtggttga tagaatgctc gagatggcgc gggtgactcc cgccgacgtg
    14281 gtctacgatt taggcagcgg cgacggcgca attatcattc gcgcggccca gaagtttggg
    14341 gttcggggag tcggcatcga gatcgatgcc gaccttgtgc gtaaagctca agccaatgca
    14401 tataaaaaca aggtcgagca tttggtcgag tttcgtgtgc aagatgcctt gtcagtggat
    14461 gtgtctgaag caacggtggt aacgttatac atgttgcctg aattcaacgc caaattgcgg
    14521 ccgattctcg accgtcaact aaaaccgggt gcaagagtca tatcgcatga ctttcctata
    14581 gaaggctggg ttgccgatcg gacagagcgg gtaagcggtg attttctgca cgatcacacg
    14641 attctattat ttgagatgcg ctgatgaccc gaaggagtta tgctgaatga ctaaagatcc
    14701 aagagtgcct ggtctgaaat tcaatggcga tgggtaacgc gtaatctgca cgggcgcggt
    14761 gtgcgttaac ttaatcaatt tcgtaactga ttgctgaggc cacgtcgagc tgggagcact
    14821 cgccgtggct ctcggcatga tcggctgcct aatactcatt ccgctgacgt tccaggcgtt
    14881 gaatagtctg tcggttgcga gtgatttcag cctgatttct ttgaatctgc tgttgctgct
    14941 gagaattggt gatctcctga ccctgcagtt ggtctcccac cagcgctccg gcgcctaggc
    15001 cgaaagctcc gccgattgct gctccggcac cgggatggcc aacggcggcg ccgataagcc
    15061 cccctgccgc ggctcccccc aacgctccga ccccagctcc cttttcacgt gtggataaag
    15121 agccaccggc acaaccggtc acgaacattg ctccggccaa aagcaaaatt cttgttcttc
    15181 ctagagattg gtttcctgtg tgcatgatct tgttcctttc tatgtcacga gcatcccaaa
    15241 aacatttgat cgccttgccg cttcctttat tttttccgca tcggtttgtt ccgagcgctc
    15301 aatgaagggt cccggcgctg tatactctga ctgtcatctg aacgcaccca gccacggtcc
    15361 gggtctgccg accattgcgc gttttggttt gtgcttggtt tgctactgtt cttgctggaa
    15421 atcgcgccat gctgctgagt tggatttttt tctttttcag cggcaaggac agagatggtt
    15481 acattggcca gcagcagaat ggcgactgct agcgccggta tgtagcaggt tgtcttcata
    15541 aaacatttga ttaagctttt ttcgcttcca tcctgttcat agtcacagcg atgatagcgg
    15601 gcgcggcgaa tgccggctgg gcgagcttgt tcatatcaat tttgccaacg ttgtcgttaa
    15661 agcaacgaac aatcgcgttg agtaaggcca tgacttcgac aaaatgatct gtttgcatcg
    15721 atcccccctt agccgtcgag agctatcaac gcaactcaaa tgccagagcg aaaagacatt
    15781 gtaagtgggc tatttacggt tcttagtagc gcttgtggga gggtaaaaaa gtgatggtca
    15841 gggaagaaat acgtgatcaa agatcgattg aatatttaac ccgattgaat gcgtcagcga
    15901 tattaactta tgtccggctg tattctgtca gatcatagcg cgcagcgggt ggatgtcagc
    15961 cgctgggaga gaaagccaaa aagggaccga cacttctgag gtatgaagag gtttatgctt
    16021 tgccgtagag cgcgagcttg acactcagag tcttcggggt gataccgagc aggcgcgcag
    16081 cttgggtttt gttgccttcg cttgcagcta aagccttttc aatggcttga cgttcggctt
    16141 ccgccagaga catgggtgtc agctcagatt tttcttcgac caccgctttg aggcagaact
    16201 gttcaaaatc tctggccgtc aaagtcggcc caggagacaa gactgagaga cgttccatgg
    16261 cattgcgtaa ctctcgcaca ttcccgggcc aggagtgaga ttgcagcgcg gctgtgtaat
    16321 ccccggccca ttccggtacc ggccgatggt atttcgtggc gaagaattcc agaaaataat
    16381 gtccgagcag cggaatatca tcgccacgtt ggcgcaatgg cggcatttca agatgaaaaa
    16441 cgctcagccg ataaaacaga tcctgccgca gcttgccttc cttgaccgcg cttgccggct
    16501 cgcgattggt ggcagccacc actctgaccg agacgggaat atttgcattg ccaccgatac
    16561 gtctcacctg ccgttcttcc aggactcgaa ggaatttggc ctggacttcc agaggcatct
    16621 cagtaatctc gtcgagaaac agtgtgccgt cgtcggcttg ttcgaagcaa cccgggtgtc
    16681 gttcaatggc gttagtgaaa gcgcccttct catggccgaa aagttcgctt tccatcaggg
    16741 ttgccgagat cgccgcacag ttaagcggaa gaaatgtagc ctcgcgcctc ggtgaaagct
    16801 catgcaatgt ttgggcgacg agttctttgc cggtgccgct ttcgccggtg atcaaaaccg
    16861 acgcgtcagt gcccgctatt ttactgattt gctgatagag ctggcgcatg ggtgcagatt
    16921 ttccgatgag cgccccgtaa gaacctaggg gggactcgag tttcaggcgt aatgcagcgg
    16981 cttctctcgc aaacgcaacc tgcttcagcg ccttctcaag tgtgattcgc agtcgcggtg
    17041 gctcgagcgg tttcggtaaa tagtcgtagg cgccgagctt gatcgcttgc actgccgtat
    17101 cgacagttgc ctggccagtg acaaagacaa cggccatttg ccgtacctgt tcacccaatg
    17161 attcgataaa ttgaagtccg ctagtacccg gcatcataag atcggttata acggcatgag
    17221 gtgcttcttt gtcgcaaatt tttagggcgg ctgctgcgtc ggcagcatcg agcgtggcgt
    17281 agccccagct ttgcaggaga gccgttaagc cttggcgcgt tgcggagtca tcatccacca
    17341 caagaacaac ggcgcgcggg tgagccatag tcaattagac tccagcggca ggatcaagcg
    17401 aaacgtggct ccatgaccat tgctgtgata ggtcaatgat ccgccgtgtg cttcgacaat
    17461 caattggctg agaggcaaac caataccatt gcccgacggt ttcgttgttt tgccaaaatc
    17521 gaaaatctcc gcttgttgtt cgggcggaac cccgggccca gcatcattga tatccacgat
    17581 cagtcgagag tcatccttct ctgccgtaat gcggatcgcg ccgccgtccg gctgcgcctc
    17641 gacggcattg cgcagcagat tgtcgaaggc ttgacgtatt cgagtcgcat caccatggac
    17701 ttgccagggg ccttgaagcc ttgcttgggc gatgctgatc ttttgagctg cgcaaactga
    17761 gccaatcgct tccaagctag tcctaaggac ttcggcgaca tccactggct tatgttccat
    17821 agtcacggga gccgagtaat cggtgaaatc tcgtatgagc cgattaactt ggcgcaggcc
    17881 tgaatcgagc gctgcgatgg aacggtgttg ttggtctacg gcggcgcctg aatcgtcggt
    17941 ggattgggcc ggattacgca ggagctcgag atgaattgcc aaagcattca gaggcgttct
    18001 gacctcgtgc gccagagtgc gcgcggccag cgccagccgt tgtgcagtcc cgggtgcggc
    18061 aaccgtgttg cgctgtttca ttaccaagcg atcgatcaga tccaagctcc tgcccacttc
    18121 gtcctcgttg gcatacggcg ggcgagccgc gtaagttccc gcttccgctt ggctgagggt
    18181 ttcactgatg atacgaaaac ggcgcgtata aagcgccgta atgagaaatg cgctgaccaa
    18241 ggcgatgcca ccacccagtg caaaaagcag
//
LOCUS       DASUVS010000053         7844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_892744, whole genome shotgun sequence.
ACCESSION   DASUVS010000053 DASUVS010000000
VERSION     DASUVS010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7844
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892744"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1042)
                     /locus_tag="VF182_03000"
     CDS             complement(68..1042)
                     /locus_tag="VF182_03000"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436072.1"
                     /translation="MKSIVVNCICAAILQLAGWNVSFAQEIHMSYPGLTGESAPLWVA
                     REAGFFKGYGIDAKLVYMEGGRLSIQSLLSNTTQFMAGDAVSALSAIAGGADIVLLAS
                     AKNVLPYVFAVSKDVRRGEDLKGKIIATSQVGGRAGEIARMVIRNMGLDPDKDVNYLA
                     VGGTMARLAALSTGKVHAAPISHTVVPIAEERGLRILQIEPIPLIVDALWTSRKFADE
                     NPLVIQNVLRGYAQAIATIVRNRDKSLEIMRKYMRTSDPRVVQGAYERYREDLDRVPV
                     PSDKAIKTTLEISRRVAPKLVGMDIDRYMYFAPVQKLAAEGFIDKLYK"
     gene            complement(1057..1773)
                     /locus_tag="VF182_03005"
     CDS             complement(1057..1773)
                     /locus_tag="VF182_03005"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436073.1"
                     /translation="MFGWRARIGYLCPSVFEMIAYDFYRIVPPGVGMIGVTCMIDGWQ
                     AEAYRTGLARIKECALELSRRKCDFIIHAGVPLVVSQGNGYEAELIKTIETLTNTPAT
                     TSIVAGMEALKALSVRRIGLVNPYPSELNATVVAFMKAHGFEIDAVVGLGADFTRIGD
                     VSEADVYAAAKKAVRQSPHLEGLYLPCPQFPALDVIDAIERDLGIPAVGHLTSEVWMA
                     FKRLNIRNSVSGFGKLLTMM"
     gene            complement(1803..2045)
                     /locus_tag="VF182_03010"
     CDS             complement(1803..2045)
                     /locus_tag="VF182_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436074.1"
                     /translation="MAKIRAVITEGDRQNGYKRVQVLFGTNYFLEIVENDGRVEFLLG
                     AHHTGFKADASEVNGELQKYISEVQQRHPESAFEQE"
     gene            complement(2062..2778)
                     /locus_tag="VF182_03015"
     CDS             complement(2062..2778)
                     /locus_tag="VF182_03015"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436075.1"
                     /translation="MFGYRARIGYISPSVMELNAYDFYRIVPKGVGLIAVTCMVEGWK
                     EDAYQRALAQVEACARELGRRACDFVIHGGAPLVLSQGKGFETRLLTQLQELSGAPCT
                     TSIVAAMDAFRDLSATRLAVVDPYPADLNAKMTAYLKDWGFEVASLVSLGTSFTESSI
                     ASIADIYRAAKRAIKEGRNAQAVFIPCANFPVIDVIEDIETDFGLPVISNITSQLYVG
                     FKTIGMRERVPGYGKLMRML"
     gene            complement(2817..3554)
                     /locus_tag="VF182_03020"
     CDS             complement(2817..3554)
                     /locus_tag="VF182_03020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6436076.1"
                     /translation="MDRLCIVTGGANGIGAAIVKRLHEDGYHVLFCDVDRENGERLAK
                     DLSRNEHAVSFVPCDIKSDNDVGRMEENVAGLGIQLYALVNNAGIFPRRQFLDISLQE
                     WTDVIATNLTAPFLLAQRLVPLMIPNGDGVVVNIGSGQSFRGDARGVHYTSTKHAIRG
                     LTKSLALALGPNGIRVNAIAPGPTLTSQALQVRSREELLSRGRQLPLGRIGQPNDIAG
                     VVSFLLGSDARYLTGQIIPVNGGSDMP"
     gene            complement(3659..4687)
                     /locus_tag="VF182_03025"
     CDS             complement(3659..4687)
                     /locus_tag="VF182_03025"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX6436077.1"
                     /translation="MKLSNTENGQSMAELNYKAATEPFDNVLHHYWHAVARSQDVTDK
                     PLPVKLLDHPVVLWRADTGVVAFHDLCVHRGTPLSLGWTENGSIVCAYHGWRYGSDGL
                     CKHIPSIPADRGIPAKARAIVYRSAEKYGLIWVCLKDPVADIPAFPPEIDDPSYRWEA
                     YSSHGQWKANAARMIENLLDFSHFTWVHPGILGDPSNALCESSEITPVPGGFQYEIEQ
                     PVNRLKANSASIQRFTVILPLTLFIERRQPDGPEKQTNIYCCTPMSDKETRFFRLSGR
                     NYRDALSDEELNKKHQTIFEQDRVIVEAQRPEELPLDLAEELHLRGPDTAGLEYRRAL
                     REIGVKWS"
     gene            complement(4742..5794)
                     /locus_tag="VF182_03030"
     CDS             complement(4742..5794)
                     /locus_tag="VF182_03030"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6436078.1"
                     /translation="MFGMRVNKIIGSLAAALSIFALIGSAVAQPSKVADFYRGKQLRF
                     ISGYSAGGLFDSATRIMARHMGKFIPGNPNVWVDNMTGAGGLIATNYVAKAAPKDGTV
                     LLNLDGALLRLQAFNNPSAQFDARQFNWLSSPAPDLQVCWVAKESGWTSITQALGGSK
                     ELKLGGLAPGTFPSDTARILQEALGIKIRLVDGFKGVSEVRLATESNEVDGSCSSYEG
                     VKRAFPKELETGEIQVIVQIGETSWPGLEKVANAMDLVKTERAKQLLRVAVIGPNDIN
                     RLFTLPPGVPADRVEALRQAFDATFNDPEYKADVAKARLVLRPISVEKIKEVASSWLD
                     MPDAQKKELQQILQIK"
     gene            complement(5908..6888)
                     /locus_tag="VF182_03035"
     CDS             complement(5908..6888)
                     /locus_tag="VF182_03035"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436079.1"
                     /translation="MIAILITLTFLGFSCPARALAQTPFLHSYSGTSSAQLAIWAAKD
                     LNLFSKYGLDVDFVFIAGGARGMQALVGGSTQSADSDGVAPIHVIHRGGDAVIVAGLN
                     NKPLFKFVTQKEITEPAQLRKKRIGVANFGGSTEFAVTTALKEWNIPREAVTLVAAGG
                     SAVRLAAMEKKGLDATVLPYDHAAAGSKAGLRILADMPTLVPSFPDKVIIMRRSFVQK
                     ERDTVKRFLQGLSEAIYQVSISPEKSMSILSKRLGVKDPKVIEENYNIYGRLFAFPPR
                     VVRKGMDSVLEQIQQQSGDAKSDFEFGRFVDESIIDQLERDGFFKRISQN"
     gene            complement(7148..>7844)
                     /locus_tag="VF182_03040"
     CDS             complement(7148..>7844)
                     /locus_tag="VF182_03040"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HEX6436080.1"
                     /translation="VDVVQCETIDVQVPATSEIVLEGELTAGERRTEGPFGEFPGYYQ
                     AVTEQPVFKLKAITHRRNPIYVTALTGPPTTDNHVMREIVLEAVLYDRIRQICPTVRD
                     VCVTKGGVNLHVVVSIRPTFIAQARDVMLACFSTERIRPKLVTVVDEDIDPRDPVMVE
                     WAMATRFQADRDLTVLPRQVGAPLDPSTPAPRLGAIMGIDATRPFGQEFPEVAVVPGA
                     EDFVIPGWTDRRR"
BASE COUNT         1795 a         2257 c         1960 g         1832 t
ORIGIN      
        1 gttacgattg ccaaatgacg aatacgcgcc ataattatca agcctccctc tctcgtgtat
       61 tttaaattta cttgtacaac ttatcgatga agccttccgc cgccagtttc tgtaccggag
      121 caaaatacat atatcgatca atgtccatgc cgaccagctt cggagccact cgacggctta
      181 tctccagtgt cgtcttgatc gccttgtccg agggtaccgg tactcggtcc aggtcttctc
      241 tgtagcgttc gtaagcgcct tgcaccaccc gcgggtccga ggttctcatg tacttgcgca
      301 taatctccaa ggacttgtcg cggttcctca cgatcgtggc tattgcctga gcataaccgc
      361 gcagcacatt ctggataacc agcgggtttt cgtcggcgaa tttccgtgaa gtccaaaggg
      421 catcgacaat cagcgggatg ggttcgatct gcagaattct aaggcctcgc tcctcagcga
      481 tgggaacaac ggtatgcgag atcggcgccg cgtgcacttt gccggtgctt agagccgcca
      541 gacgggccat ggttccaccg acagccaggt aattcacgtc tttgtcagga tccaaaccca
      601 tattgcggat taccatgcgg gcgatctctc ccgctcgtcc gccgacttgc gaagtcgcga
      661 ttatcttccc tttgagatct tcgccgcgcc gaacgtcctt ggacacggca aacacataag
      721 gcaatacgtt tttagccgaa gccagcaaaa cgatgtccgc accgccggca attgcactca
      781 gagccgagac ggcgtcacct gccataaact gagtcgtgtt cgagagaagg ctttggatcg
      841 ataagcggcc tccttccata tatacgagct tggcatcgat gccatatccc ttgaaaaatc
      901 ccgcttcccg ggcaacccac aacggcgcag attcgccggt cagcccaggg taagacatgt
      961 gaatctcctg agcaaaagaa acattccagc cggccagttg cagtatcgcc gcacagatac
     1021 aattgacgac aatcgatttc acagatcctc cagtgctcac atcatggtca ggagtttgcc
     1081 gaatccggag acggaatttc gtatatttaa tcgcttaaat gccatccaca cctccgacgt
     1141 caaatgacct actgctggaa tgccgaggtc gcgctcgatg gcatcaatga catcgagcgc
     1201 tggaaactgc gggcaaggca aatataaacc ttccaagtga ggcgactgcc tcacggcttt
     1261 tttcgctgcc gcgtaaacat cggcttccga gacatccccg attcgggtga aatcagctcc
     1321 gaggccgacg actgcgtcga tttcaaatcc gtgcgctttc atgaatgcca ctaccgttgc
     1381 gttcaattcc gatggatatg gatttacaag gccgatgcgc cgaacagaaa gagctttcag
     1441 cgcttccata ccggcaacaa tgctggttgt ggccggagta ttggtaagcg tctcgatggt
     1501 tttgatcaac tcagcctcgt aaccatttcc ttgactcaca accagcggga cccctgcgtg
     1561 aatgatgaaa tcacattttc gccggctcaa ctccagcgcg cattctttaa tccgcgccag
     1621 tccggttctg tacgcctcag cctgccagcc gtcgatcatg caggtgaccc cgatcatgcc
     1681 gactccaggc ggaacgatgc gatagaaatc gtaggcgatc atctcgaaca cggagggaca
     1741 aaggtagcct attctggcgc gccaaccgaa catatagttg tacctttcgg gttgtctgtt
     1801 acctattcct gctcgaaggc gctttccgga tgccgctgct gaacttcgct gatatacttt
     1861 tgcagttcgc cattgacttc ggacgcatcc gctttgaatc cggtgtgatg cgctcccagc
     1921 aggaactcaa ctcggccgtc gttttcaaca atttccaaga aatagtttgt gccaaataat
     1981 acctgaactc gcttgtagcc attttgacgg tccccttcgg tgatcaccgc tcttatcttc
     2041 gccatttcct ctcctccttg attaaagcat tcgcatcaac ttgccatatc ccggaactct
     2101 ctcgcgcatt ccaatagttt tgaatcctac atacaattga gaagtgatat tgctgatcac
     2161 cggcagacca aaatccgttt caatatcttc aatgacatca atcaccggga aattcgcgca
     2221 gggtataaag acggcttggg cgtttcggcc ttctttgatc gcgcgtttgg ccgccctata
     2281 gatatcggca atcgatgcga tgctcgactc ggtaaaagac gtccctaggc tgaccaggga
     2341 agcaacctca aagccccagt ccttcagata tgcggtcatc ttggcgttaa ggtcggcggg
     2401 atacggatcg acaaccgcca atcgcgtagc tgaaaggtcg cgaaaagcgt ccatggccgc
     2461 gacaatgctg gtcgtgcacg gagcaccgct gagctcttga agctgggtca gtagtcttgt
     2521 ttcaaatccc ttgccctgac tcaaaaccaa aggagcgccg ccgtgaatca cgaaatcgca
     2581 agcgcggcgg ccaagctccc gggcacatgc ttcaacttgc gccagcgcgc gttgataggc
     2641 atcttccttc cagccctcaa ccatgcaggt gacggcgatc aggcctactc ctttggggac
     2701 gatgcggtag aaatcatagg cgttgagctc catgacagac ggcgaaatgt aacctattct
     2761 ggcgcggtat ccgaacatac aaaactcctc gtaagagtgt tattaactag gctctttcac
     2821 ggcatatccg atccaccgtt aaccggtatg atttgacctg tcaggtatcg agcgtcactt
     2881 cccaagagaa atgacacgac tccggcaatg tcgttaggtt gtccgattcg tcctaagggc
     2941 aattgccgac cacgactcag gagttcctcc cgcgaacgga cttgcagcgc ctgagacgta
     3001 agcgtcggac ccggagcaat ggcattcacg cgaatcccgt taggacccag cgccagcgcc
     3061 agagatttgg ttagaccccg aatcgcgtgt tttgtcgatg tatagtggac gccgcgagca
     3121 tcgcccctga aagactgccc cgatccaata ttaacaacga caccatcgcc gttagggatc
     3181 atcagcggga ctaagcgctg ggctaacaga aaaggtgcgg tcaagttcgt cgctatgaca
     3241 tcagtccact cctgcaggga aatgtccaga aactgtcgtc tgggaaatat tcccgcatta
     3301 ttgacgagcg catagagttg aatcccaagc ccggctacat tctcctccat ccggccgaca
     3361 tcgttgtcgc ttttgatatc acatggaacg aaggacaccg catgctcgtt acgacttaaa
     3421 tccttggcta gccgttctcc attttctctg tccacatcgc aaaacagcac gtggtagccg
     3481 tcttcgtgca gccgcttcac gattgccgcg ccaatgccgt ttgctccgcc ggtaacgata
     3541 catagccgat ccatattatt tccgcatcag ttcaggatgg aatcacttct cagcgtttct
     3601 cggtgatgtt gataactcaa aggcttgcaa gtagaggagc ctggggacgc gacaagactc
     3661 aactccactt cacgccaatt tcgcgcaggg ctcttcgata ctctagaccg gccgtatcag
     3721 gcccgcgcag atgaagctct tccgctaaat ccaggggcag ttcctccggc ctttgagcct
     3781 cgacgatgac gcgatcctgc tcgaagatcg tctgatgttt cttgttgagt tcctcatccg
     3841 aaagcgcgtc tcgataattg cgaccggaca ggcgaaaaaa tctcgtttcc ttatcggaca
     3901 tcggagtgca gcaataaatg ttcgtttgtt tctcgggtcc atccggctga cgcctttcaa
     3961 tgaacaaagt caatggcaga atcaccgtga agcgctgtat cgacgcgcta tttgctttca
     4021 agcggttaac cggctgttcg atctcatact ggaacccccc cggcacgggc gtgatctcgc
     4081 tcgactcgca taaggcgttc gaaggatcgc ccagaattcc cgggtgcacc caagtgaaat
     4141 gcgagaagtc caaaagattc tcgatcatcc gggcggcatt ggccttccat tgcccgtgcg
     4201 acgaatacgc ctcccatcgg tacgacgggt cgtcaatctc cggaggaaac gccgggatgt
     4261 cggcgaccgg atcttttaga catacccaaa tcaacccgta cttttcggcg gagcgataaa
     4321 cgatcgctcg cgccttggct ggtattcccc tatcggccgg tatggagggg atgtgtttgc
     4381 acagtccgtc cgaaccatac cgccagccat ggtaggcgca tacgatactg ccgttttcag
     4441 tccaaccaag agagagaggg gtgccgcggt gtacgcagag atcatgaaag gcaaccacgc
     4501 ctgtatcggc gcgccaaagc accaccggat gatcgagtaa tttcaccggc agcggtttgt
     4561 ccgttacatc ttgcgaacgc gctaccgcat gccaatagtg gtgcagaaca ttgtcgaacg
     4621 gttccgttgc cgccttatag ttaagttcgg ccatcgactg gccgttttcc gtgttcgata
     4681 atttcatcat actctcctaa tcggcaaatt ctcgactgac accagagtca atgcacccgg
     4741 attacttaat ctgcaaaatt tgctgcagct ctttcttttg agcatcaggc atatccagcc
     4801 acgaagaagc tacttcttta atcttctcga ctgaaatcgg tcgcaagacc aagcgggcct
     4861 ttgcgacgtc ggctttgtat tccggatcgt tgaaggtcgc atcgaaagct tgccgaagcg
     4921 cttctactcg atcggccggc actcctggag ggagcgtaaa caaacgattg atatcgttgg
     4981 gaccgatgac ggccacgcga agaagctgct tggctcgctc ggttttaacc aggtccatcg
     5041 cattagccac tttctctaga cccggccagg aagtttcacc gatctggaca atcacttgta
     5101 tttcacctgt ttccaattcc ttcggaaagg ctcgtttgac gccctcgtac gaggagcacg
     5161 aaccgtccac ttcgttgctc tctgtggcaa gtcggacttc ggaaacacct ttgaaaccgt
     5221 ctacaagcct tatcttgatc cccaacgcct cttgaagaat tcgcgcggtg tcggaaggga
     5281 aagtacctgg cgcaagcccc ccgagcttca actctttaga gccgccaaga gcctgggtaa
     5341 tgctggtcca accactttct ttagccaccc aacacacctg gaggtccggc gccggagacg
     5401 aaagccagtt aaattgacga gcatcgaatt gcgccgacgg gttattaaac gcctgtagcc
     5461 tcaatagcgc tccgtcgaga ttcaaaagca ccgttccgtc cttaggcgca gccttggcga
     5521 cgtaattcgt cgcgatcaga ccgcccgcgc ctgtcatatt atcgacccat acgtttgggt
     5581 tgccgggaat aaacttaccc atgtgccgcg ccataatacg agtcgcgcta tcgaagagtc
     5641 cgcccgcaga gtaaccgctg ataaagcgca actgtttgcc gcgataaaaa tcggccacct
     5701 tcgatggttg cgcgacggca ctgccaatca gtgcaaatat cgatagcgcc gcggctaggg
     5761 aaccaattat cttattcact ctcatcccga acataatcac tccttgtatt gcgatcgcga
     5821 cgccactcgg cagatgccgg cagtcatgct cgctacctgt ccatcctgca atcaagctcg
     5881 atgctttgaa gcaaacctag cgagctatta gttttggcta atcctcttaa aaaagccgtc
     5941 tcgctccagt tgatcaatga tactttcatc gacaaaccgt ccgaactcga agtcggactt
     6001 tgcgtcaccg gactgttgct ggatctgctc taaaacacta tccattccct ttcgaacaac
     6061 tcgcggcggg aacgcgaaca gccggccgta tatattgtag ttctcctcga tcactttcgg
     6121 atctttgacg ccgagccgtt tgctcagaat tgacatgctc ttttcaggac tgatcgaaac
     6181 ctggtaaatc gcttcgctca atccttgtag aaatctttta accgtgtcgc gctctttttg
     6241 aacaaacgag cggcgcatga tgatgacctt gtccggaaaa ctgggcacca aagtgggcat
     6301 gtccgctaaa attctcaaac cggctttgct cccggcggcg gcatggtcat aaggaaggac
     6361 ggttgcgtcc aaacctttct tttccattgc cgccaggcgc acggcacttc cgccggcggc
     6421 tacgagggtc acggcctctc tcggaatatt ccattctttt agcgccgtgg tcacggcaaa
     6481 ttctgtcgat ccgccgaagt tagcgacgcc gattcttttc ttgcgcagct gcgcaggctc
     6541 ggtaatttct ttttgagtta cgaatttaaa caaaggcttg ttgttgagac ctgccacgat
     6601 gacggcgtcc ccgccgcgat gaatcacatg gatcggcgcg acgccatcgc tgtcagccga
     6661 ctgagtgctc cctccgacca gggcctgcat gccacgcgcg ccgccggcaa taaacacaaa
     6721 atcaacatcg agtccgtatt tggaaaaaag atttaaatct tttgccgccc agatcgcgag
     6781 ctgcgcgctg ctggttcccg aatagctatg aagaaaaggc gtttgggcga gagcgcgtgc
     6841 cggacaactg aaacctaaaa acgtcaacgt gattagtatt gcgatcactt tctcttcctt
     6901 cctggctcgt ctgttagccg tgcccaatta cggtgtcgcc gcgtcaaacg cgaatgattt
     6961 aaggccagcg ctcgtggctg cgagttttcc gctgctggag atcaggtgca aaaatatttg
     7021 cctattcttc actcacgctc tgcaccccag caaggcagcc ccgagtgcgt ggttttagtt
     7081 tgcgttcaaa gatttaaaaa cacgcgccga cctgatagca gatcgccctt gcggatcgaa
     7141 gcacctctca ccgccggcga tcggtccagc cggggatgac aaaatcttcc gcgccgggaa
     7201 caaccgccac ctcgggaaat tcttgaccaa atggacgcgt tgcgtcgatg cccatgatgg
     7261 cgccgagacg gggcgcggga gtggatggat ccagaggcgc gccgacttgt ctcggcagca
     7321 ccgtgagatc gcgatccgcc tgaaagcgcg tcgccattgc ccattcgacc atgaccggat
     7381 cgcgcggatc gatatcctcg tcaacgaccg tcacgagctt gggccggata cgctcggttg
     7441 aaaaacaagc cagcatgacg tcgcgggctt gggcgataaa agtcggcctg atcgaaacaa
     7501 ctacatgtaa attgacgccg cctttggtga cgcagacgtc gcgcaccgta ggacagatct
     7561 gacgaatccg gtcatacaac acggcttcaa gaacaatttc tctcatgaca tggttatcgg
     7621 tggtcggcgg gccggtgagc gcggtaacgt aaatgggatt tcgccggtgc gtaatcgctt
     7681 tcaatttaaa cacgggctgt tcggtcactg cctggtagta gcctggaaac tcgccgaacg
     7741 ggccttcagt gcggcgctcg ccggcagtca attctccctc gagtacgatc tcgctggtgg
     7801 cgggcacctg gacgtcgatg gtctcgcatt gcacgacatc gacc
//
LOCUS       DASUVS010000054        25066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_675089, whole genome shotgun sequence.
ACCESSION   DASUVS010000054 DASUVS010000000
VERSION     DASUVS010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 25066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25066
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675089"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VF182_03045"
     CDS             complement(<1..730)
                     /locus_tag="VF182_03045"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HEX6436081.1"
                     /translation="MSDIEKKDQLEEYFHAAGKPRERWRVGTEYEKVGIDRSSGKAIP
                     YSGPRGVESILKRLIDRFGWEPEEQDGHIIALSRDNAQITLEPGGQIELSGEPCDSIH
                     CTYAEFTQHIRELLEVSDPLDVVFLGLGMQPVSRLEEIEWVPKKRYRIMGPYMPKVGR
                     LGQRMMKQTATVQANIDYRDEKDAMSKFRTGMGLTPIFISMFANSPICDGQLNGYRSY
                     REHIWTDTDKSRSGMLKFAFSRDVS"
     gene            complement(717..1748)
                     /gene="mtnA"
                     /locus_tag="VF182_03050"
     CDS             complement(717..1748)
                     /gene="mtnA"
                     /locus_tag="VF182_03050"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX6436082.1"
                     /translation="MRVKTIEWKNDQVVMLDQRLLPHKEINRVCRDYKEVAAAITHMV
                     IRGAPAIGVAAAMGVALGALKSKAKNFDEEFNEIVSTLAKTRPTAVNLFWALERMKKV
                     YLENRDRGVEAVKRVLKIEAQNIYKEDIAANKKIGAFGAKLLTDAKHVMTHCNAGALA
                     TAGYGTALGVMRALKDSGQKFEVLVNETRPFLQGARLTAWELKKEKIAATLITDNMSG
                     YLMQTGRVDAVVVGCDRIAANGDVANKIGTYTIAVLARRHGIPFYVAGPTSSIDLNCP
                     TGKDIPIEQRDPKEVSHMFGRPLAPEGMNILNPAFDVTSQELISAIITEKGIIYPPYR
                     QNIENHVRH"
     gene            1815..2057
                     /locus_tag="VF182_03055"
     CDS             1815..2057
                     /locus_tag="VF182_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436083.1"
                     /translation="MDFYHLLGYLSGLLFIKPNMEATSLYRTIALVHCLDAILCSVIA
                     AHSGRRTVGWSIGGLIFGIWALGLLFLLPIKKPAGA"
     gene            complement(2102..2491)
                     /gene="acpS"
                     /locus_tag="VF182_03060"
     CDS             complement(2102..2491)
                     /gene="acpS"
                     /locus_tag="VF182_03060"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX6436084.1"
                     /translation="MIISVGIDLVEVERIRAALEHPRIGIRFRERVYTPREIAYCEKR
                     QRGRFQSYAGRFAAKEAVMKALGRGWGAQVRWLDIEVARANSGKPDVVLHSKTAQLAE
                     KLGIKHWALSITHTDQHGMAYVIAQDE"
     gene            2620..3798
                     /locus_tag="VF182_03065"
     CDS             2620..3798
                     /locus_tag="VF182_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6436085.1"
                     /translation="MIARRTLLFSLMLLFGIPLPATAQKGTIKLAVQAPLSGEQAALG
                     EHIKLGAQLAVEEASKSFKSMGYDLVFVPYDDQAKPEVGVANARNLVADPDVLILVGH
                     FNSGVALPASEVYKDAMLAMISPANTATEITDRGYPNVNRVCGRDDVQGPVGARFAAQ
                     DLKFKSVYIIHDKTLYGQGVADNFRNEAKKLGLNVLGYDGTEERANFAPMIIPLKAKN
                     PDLVYFGGIYHQGGLLLKQMREKGVKSAFMGPDGLDSSEMVKIAGQHVIGSYYTTVAG
                     PPDAYPETAAFAKKFKQRFGKEVESFAMYGYDAAQVGIKAVEQTLQTNGGKKPSRGEV
                     SAAVRKLDNFRGVTGQISFDNKGDPVKAKYFVLQFDKKNYPGKVVKVIEQQAPQAKKS
                     "
     gene            3864..4949
                     /gene="ddlA"
                     /locus_tag="VF182_03070"
     CDS             3864..4949
                     /gene="ddlA"
                     /locus_tag="VF182_03070"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX6436086.1"
                     /translation="MKNKLRVAILFGGRSAEHEISLISARNIVAAMDKSRYEIVAIGI
                     DKQGRWSYDREARLLGDGQKQVIPFNHAQLTGILPGASQSSLIALGGARLKIDVVFPV
                     LHGPFGEDGTVQGLLKLANIPFVGASVLGSAIGMDKDVMKRLLRDARVPIGRFHVYGR
                     SAADRIKFTPLTKDLGQPLFVKPANLGSSVGISKVEKRSELEAAVRNAFRFDNKILIE
                     EFIEGREIECSVIGNDDPIASLPGEIVTGHNFYSYDAKYIDDQGSRLEIPAKLPRAIV
                     KSVREIAIKTYRTLCCEGMARVDFFVKANGQVLVNEINTIPGFTQISMYPKMWAASGI
                     SYPKLIDRLIQLALQRFRQEQKLRTSV"
     gene            complement(5232..6497)
                     /gene="ftsZ"
                     /locus_tag="VF182_03075"
     CDS             complement(5232..6497)
                     /gene="ftsZ"
                     /locus_tag="VF182_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX6436087.1"
                     /translation="MFEFVEEFNLNARIKVIGIGGGGGNAVNTMIGGKLNGVEFLVAN
                     TDAQSLDASRAPVRIQLGGAVTKGLGAGANPEIGRRAALEDQETIKEYLAGSDMIFIT
                     AGMGGGTGTGGAPVIARVAREVGALTVGVVTKPFIFEGKKRMRQAEEGIAELKSSVDT
                     LIVIPNQRLLSIAAKTTTMLEAFHKADEVLLQAVRGISDLIITPGLINLDFADVRTVM
                     AEMGLALMGASTATGENRAIEAAQRAISSPLLEDISIQGARGVLINITGGPDLCLHEV
                     NEAASMIQEEAHDEANIIFGAVIDETMLDEIRITVIATGFGEGKDERKPVITSSGHVS
                     SMNNVNSMNHVTSSVPNVANVNPVKTKKVVHLGTIVDDLDAPTWQRKKPGSEEVETVT
                     LSKSTLQLSTGQEEDDKYDIPTFLRRQMD"
     gene            complement(6541..7770)
                     /gene="ftsA"
                     /locus_tag="VF182_03080"
     CDS             complement(6541..7770)
                     /gene="ftsA"
                     /locus_tag="VF182_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX6436088.1"
                     /translation="MAKKHSIIVGLDIGTSKVCSAVGEMTEHGVEIIGIGSHSSLGMR
                     KGVVIHIDNTVASIKKAVEEAALMAGCEINTVFTGIAGSHIKAFNSHGVVAVKTREVS
                     QRDLDRVLDAAKAVAIPTDQEIVHVLPQDYIIDDQDGIKDPLGMSGVRLEARVHVVTG
                     ATTAMQNVIKCCNRSGLHVADVALMPVACADAVLTEDEKDLGVALADMGSGTTDITIF
                     HDGTVKHTAVLPIGGNHVTNDIAAGLRTPFADAERIKLRYGCAMASMVAEQVRLEVPS
                     SAGKPQRSVSRQILCEIIEPRMDEVFQLIRREIVKSGYEDYLGSGVVMTGGSMLLPGA
                     IEAAELAIGCPVRLGTPRHVGGLVDVITNPVYSSAIGLVLYGIKRQERSFFRVREDAT
                     ILSKVKHRMSDWLSDFF"
     gene            complement(7797..8612)
                     /locus_tag="VF182_03085"
     CDS             complement(7797..8612)
                     /locus_tag="VF182_03085"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX6436089.1"
                     /translation="MQLAVSRKDNRKKDKFQWRRRLMVAGIMIMFVVAAWTVYGWREQ
                     VGLASVAVRRFIFENPYFAVGEIQVRGSDKVRGNEIISMAGLRHGINLWSIDPAKIEK
                     KVSQHPWVRRVLVRREFPRRVVIEVEERRPKAIVAVGKLYYVDADGVLFKEVAQGENV
                     GFPMLTGIRTEELGSFDPAVRRRIQDALRLGDLMAKDSHALSEIHFDAPDRLVVYTIA
                     HPVAIHMGWGDWEGKLQRLDRVMTLWRGKEERLGSLDISFQDRVVTRLRRQPQ"
     gene            complement(8618..9577)
                     /gene="murB"
                     /locus_tag="VF182_03090"
     CDS             complement(8618..9577)
                     /gene="murB"
                     /locus_tag="VF182_03090"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX6436090.1"
                     /translation="MLAMNPKPNETNLVRELQSISGLRVKVSEPLARYTSMKVGGPAD
                     YFVEVESEPALAQLLCLLSLRGMPFRLLGNGSNVLISDLGIEGVLIHLIGAFKNAEWH
                     EQANESWVDAGAGQAVTQLVRQAARRGYGGLEFAEGIPGSVGGALVMNAGAYGSEFEK
                     VVVAVYGMTRQGTPIQLNREEMRFTYRDSHLPAGIVVTRVRLSLHRQESSRVSIKVRE
                     LVIKRKSSQPSGYPNSGSMFRNPPDDYAGRLVEAAGLKGKQIGRAQISPKHGNFIVNL
                     GGASAEDVRRLMELARAEVQAKFGVVLIPEVKLIGRWPSLRVE"
     gene            complement(9661..11031)
                     /gene="murC"
                     /locus_tag="VF182_03095"
     CDS             complement(9661..11031)
                     /gene="murC"
                     /locus_tag="VF182_03095"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX6436091.1"
                     /translation="MLHKKHRVHFVGIGGIGMSGIAEVLLNSGYVVSGSDLHDSDATR
                     RLRKLGAQIFVGHQEDNLTVNPSVVVISTAVKYSNPEVLEARRRQIPVIPRAEMLAEL
                     MRMKYGIAVAGSHGKTTTTSMTAAVLSLAGLDPTMVIGGRVHMLGTNAKMGQGEILVA
                     EADESDGSFLLLTPTIAVVTNIDREHMDFHQTMERLNESFLTFVNKLPFYGLAVLCID
                     NPNVRALLPKVRKRFATYGLSPEADFSVQDLHMKAGGVEFTVLHHGNPLGQLKLQLPG
                     RYSATNALAAVAVAHELEIPFCRTAEALAAFNGIHRRFEIKGQTRDVLIIDDYGHHPV
                     EVRATIGAIRESWKRPLTVIFQPHRYSRTQDLFDDFLTAFEGADRLVLTEIYGAGEDP
                     VPGVTGEALYQAIKRQGHMDVEFIADKTSIAPQLAERLIAGDVVLTLGAGDIYKVGES
                     ILAALA"
     gene            complement(11055..12122)
                     /gene="murG"
                     /locus_tag="VF182_03100"
     CDS             complement(11055..12122)
                     /gene="murG"
                     /locus_tag="VF182_03100"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX6436092.1"
                     /translation="MRVILAGGGTGGHLFPGLAVAREFQRRDAMTEILFVGTDKGIEA
                     RVLPREGFPLELIAVKGLKGRGVRGLWDALYGIPAGLLQSLGILKRFRPDCILGLGGY
                     ASGPLLVAAKLRGIRCAIMEQNLQPGFTNKMLARFVDKIFTSYRETASYLPAQKVLET
                     GTPVRWHRLPNAPKGEKFTLLIFGGSAGAHRINIAVIDALKQMIDLASQFRIIHQTGA
                     ADFPAIGKAYQDLPFDAEALPFIDRMDDAYARADLVLCRAGATTVAELTAFGKAAILI
                     PYPYAIYDHQRWNAQALADKGAAEMILDHELNGAALESRLRAYVADPTKIEPMATAAR
                     AMGKPEAAARIVDECYALAHT"
     gene            complement(12123..13217)
                     /gene="ftsW"
                     /locus_tag="VF182_03105"
     CDS             complement(12123..13217)
                     /gene="ftsW"
                     /locus_tag="VF182_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX6436093.1"
                     /translation="MRDALAVDKWIFFSVVALTALGLTMVLSTSYLYAQERFGDGIYF
                     FRKQSIAAGAGLVGLVIGSLIPPALLRRLAYPLLAVTLVVLVLVLIPGIGLMRGGARR
                     WLMLPGFAFQPGELAKLALVFYLAHSMARKQEKIKTFSVGVLPHLIVAGAVLGVLLLQ
                     PDFGTALILAVLLYLMLFVGGARIPHLLGTGALALPVLAYVMLTAEYRLRRLMTFMDP
                     WRDASSSGFQVIQSLIAFGSGQLWGRGLGESRQKLFYLPEAHTDFVYSVIGEEMGLLG
                     ALAVLALFGVILMRGLRLTAKIEEPFEQYLAFGLTVLLGMQGLVHMGVVMGLMPTKGL
                     VLPFISYGGSALVVNLAEVGLLLGLSRRRL"
     gene            complement(13214..14548)
                     /gene="murD"
                     /locus_tag="VF182_03110"
     CDS             complement(13214..14548)
                     /gene="murD"
                     /locus_tag="VF182_03110"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX6436094.1"
                     /translation="MDLRGKKVMVLGLARTGLATARFLAQQGAVVLVSDLRPAADLQA
                     EVNALAGLPIEYHLGGEDVDWLQGVDMVVPSPGVPMNNFLLENAGQRGVEIISEIELA
                     YRFLPLPLIAVTGTNGKSTTTTLIGEILKANGTKTFVGGNLGMPLIGCGADNWEWGVV
                     EVSSFQLEWVKWFRPRIAVLLNLSEDHLDRYADFAAYASAKQRIFAAQDEPDIAILNR
                     DDSRVWAMRRQLRSQIVSIGSSEVGFGVFAERDAIVWRDGPDEETFSLTAVKIKGVHN
                     VENMMAAIAATKAVGVGHDVIQETLESFPGLEHRLEFVREKDGVRYFNDSKGTNVGAV
                     EKSLASFSEPVILIAGGVDKGGDYGPLQNCMRQRVRRLVLFGAAKDVIAEALGHLTET
                     VIVKDLAAAVADAAAHARPGDVVLLSPACSSFDMFRNYAERGKVFKSLVRDL"
     gene            complement(14563..15639)
                     /gene="mraY"
                     /locus_tag="VF182_03115"
     CDS             complement(14563..15639)
                     /gene="mraY"
                     /locus_tag="VF182_03115"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX6436095.1"
                     /translation="MLYYLLFPLHTTYSGFNVFRYITFRAAMAALVALLISFLLGPRL
                     IRALTQKQIGQHIRDDGPASHAVKAGTPTMGGALILLALTFSTLLLGDIGNRYVLLAL
                     LATVGAGLVGFVDDYLKLTRKNSKGLPPRAKLLGQFLMAFVVAVALYYMPKFSTEISL
                     PFIKNFRPDLGILYVPFAMFVIVGASNAVNLTDGLDGLAIGPVMVAAGTYAVIAYVTG
                     HMKLADYLQIPYVAGVGELSVFCAAVVAAGLGFLWYNAYPAQMFMGDVGSLALGAALG
                     AVAVMTKNEILLIIVGGVFVVEALSVIFQVASYKLRRKRVFLMAPIHHHYELKGWKEP
                     QIIVRFWIIAFICAVIGLSTLKLR"
     gene            complement(15647..17041)
                     /gene="murF"
                     /locus_tag="VF182_03120"
     CDS             complement(15647..17041)
                     /gene="murF"
                     /locus_tag="VF182_03120"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX6436096.1"
                     /translation="MAWTLEEILMATGGRLEKRASASRFDEIVTDSSKVKTGSIFIAL
                     KGERLDGHRFVKDAISRGAGCLVVHQALRAVDLRSVTAVKVPDTLRALGDLAHFRREQ
                     YRPKVLAITGSNGKTTTKEMLAAILQCASLDGQPLQGRLLTTEGNFNNLVGLPLTLLR
                     LRKRHRVVVVEMGTNRPGEIQRLAEIAAPDVALITSVAPAHLEGLSSLAGVAKEKGAL
                     FGGMRAGGTIVVNLDDPRVRRLGARFSGEKITYGEGGQIRAESGLLLDSGAMRFTLRA
                     GHQRAQIRLNFVGQHNIANAVGASAMAYSLGIPLPMIRRGLARVKPYSMRMQLENWRG
                     IGIINDAYNANPASMIAAIKTLAQISSRGQRIAVLGDMFELGKQSQREHRRLGNQLAQ
                     ARLDRAYLLGERAPDVRKGALRGGMKSDQVIVGESHSDIGKRLRAHLKKGDWLLIKGS
                     RGMKMETVLTELKG"
     gene            complement(17044..18582)
                     /locus_tag="VF182_03125"
     CDS             complement(17044..18582)
                     /locus_tag="VF182_03125"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX6436097.1"
                     /translation="MRLRDFLTLQNLAQAEGNLDQEVAGLVYDSRRVAKGDVFFAVPG
                     EQADGHQFLNQAIERGAIAVVVADKAKVPLGVTFARVHDVRAAMGLWAAQFYGHPGRR
                     LKIVGVTGTNGKTTTTYLIESILASAGMEPGVIGTINYRYGGKEMPAHHTTPESLDVH
                     ALMAEMAHQGARSLAMEVSSHALVQERVRGIDFDVGVFTNLSRDHLDYHKSMDEYFVA
                     KSRLFTDYLAASGKSAKAAVINGDDPRGSELIGRAQRAGITTWSYGSSDECDIKPLQV
                     HHSAAGQSGKIKVKDRVIEFSSELIGAANFDNILAAVGVGVALRIPQAAIVDGIKKLR
                     SVPGRLQRVENSRGVVVLVDYAHTPDALEKTLVAVRPLTDAKVLTVFGCGGDRDRGKR
                     PLMGEIAARLSDIVVLTSDNPRSENPESILQEIEAGMQKTGIVRLQTQGTKVDGVHGY
                     GVEADRRKAIGLALTWARAGDVVVIAGKGHEDYQILGAHKIHFDDREVAREILSSQAG
                     ALRA"
     gene            complement(18589..20538)
                     /locus_tag="VF182_03130"
     CDS             complement(18589..20538)
                     /locus_tag="VF182_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX6436098.1"
                     /translation="MRRTPRTSHVRLAVAKILFLAMFLVIGGRAFYLQALQGERLMRL
                     GQRQHLKESTVLPKRGGVYDRGGEALALSLEAQSVYARPGRIQDPTKSARDLARILKI
                     STAEIKERLTSDKPFVWIKRQISPNEAGQIQNLKIEGVGMFHEPHRYYPQGQLAGQVI
                     GFVGRDSEGLEGLELRYNEYIRGETGSSVSERDALGRRVLVEGVEGLQIPAGGDIHLT
                     LDTSVQHLAEKELEASIDKYRAKAGVAIAVEPRTGEILALANYPTFNPNNYSSASPEQ
                     RRNRAVTDSFEPGSTFKTILAAAALEEGIVEQEDLFFCEYGKFSYAGRIIHDSHPHGW
                     LPFSKILQVSSNIGFSKVADKLKKDRFFKYIEKFGFGRVTGIDMPGEAPGLLRRPETW
                     SAIDLATHAFGQGLSATPLQMVMAYAAIANGGFLMRPYVVRRALSPKGEVLVENQPHM
                     VRRVISERTARSLASMLQEVTTEGGTGTMAKVEGFNVAGKTGTAQKVDLVNGGYDAKK
                     RVASFVGFVPADEPRLVLMVLVDEPQSNVYGGVVAAPAFQNIARGALRHFGIAAKTAE
                     PIAAPLLEADIPLRRVPRRNGEAAVDSGATPDFVGLSLREAVEKARDLNVKVKIHGNG
                     YVVKQSPGPGGRWDENGMLVLNLQG"
     gene            complement(20535..20897)
                     /locus_tag="VF182_03135"
     CDS             complement(20535..20897)
                     /locus_tag="VF182_03135"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX6436099.1"
                     /translation="MRTKTASAANLNSPRPVRSVKAKQPNRSPKRKSQRHLIPICALG
                     FCLFILGLVHVWLRLQVVHMGYVLSTTTKLQGRLEQENRELKVELASLTSPDRLEAMA
                     RRRLGLVPPEKGQVIVLP"
     gene            complement(20894..21823)
                     /gene="rsmH"
                     /locus_tag="VF182_03140"
     CDS             complement(20894..21823)
                     /gene="rsmH"
                     /locus_tag="VF182_03140"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX6436100.1"
                     /translation="MKFAHTSVLVQESMGLLKPRAGERYLDGTLGGGGHAEELLLRSS
                     PDGRVLGLDRDEDALQAAAQRLSRFGDRLTLRQANFDQAKEILADINWNQVNGVLLDL
                     GVSSHQFDTPERGFSFQASAPLDMRMDRRQRLTASEIVNSFPEAEVTKIISDYGEEPR
                     ARRIAAAIATERKERRIETTLDLADLVARVKRKRDRAHHPATRTFQALRIAVNREMES
                     LKQFLEYGFELLQPGGRMVIISFHSLEDRLVKTAFRKWSDKCICPPRTLKCECGWSQK
                     AQRLTRKPIIASAEEVRANPRARAAKLRAAVRL"
     gene            complement(21823..22185)
                     /locus_tag="VF182_03145"
     CDS             complement(21823..22185)
                     /locus_tag="VF182_03145"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6436101.1"
                     /translation="MLPQNNIQDISVIDVSGDIDFREMIRIKNVIGGLIEKDRLKVVL
                     NLRAVDHINYLSIGVLLERLKLLRNLNGDLKLAGMSPFLRDIFKVVGMDRFFEDYTSL
                     EDAIESFDDDWEGDGTSH"
     gene            complement(22235..22687)
                     /gene="mraZ"
                     /locus_tag="VF182_03150"
     CDS             complement(22235..22687)
                     /gene="mraZ"
                     /locus_tag="VF182_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX6436102.1"
                     /translation="MFRGSFEHTLDGKGRLSIPSKFRDILLGKGDERIIMTNFVVDGL
                     RCLDVYPIDEWLRLEEQISQRPKFERRMVLFQNFYLGGACECLLDKQGRILIPTSLRK
                     YADLKRDVVLVSALEKFRVWDQETWKQFFADAEGKLMQDPDFLGDLGL"
     gene            complement(22980..23349)
                     /gene="rnpB"
                     /locus_tag="VF182_03155"
     ncRNA           complement(22980..23349)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF182_03155"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(23607..24335)
                     /locus_tag="VF182_03160"
     CDS             complement(23607..24335)
                     /locus_tag="VF182_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEX6436103.1"
                     /translation="MRAQIEILAALQIIDREIREHAAVKQGLLSELQEKQRFIHEKKQ
                     EIETLTAACAAKEKLRQEKDKLLHEESQKATDKRMRMNRIKNVKELQALQREIDQIRQ
                     ANGDVEEELLNMMVEIDGFKGEIKTKQETLLEAQQDWQQKQQALQAQVAAIDEAVNKA
                     SVSRQRIAEEIDASLMSRYELIFSRRGGNAVVEAMSGICQGCYMNIPPQLWNEIIRSE
                     KVNLCPSCQRILHYKAPEEHEKTA"
     gene            complement(24553..>25066)
                     /locus_tag="VF182_03165"
     CDS             complement(24553..>25066)
                     /locus_tag="VF182_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6436104.1"
                     /translation="DVLLRSGAKQLPITEAETFWRNRHAVARRFLANRSQQRARGRDG
                     FHRDWIHVALPASKVLAFRRAAMVIIERHGVDLHESGLWIQPELFSMRLGIEDHGQNQ
                     AQLALQQTVDELLKLAQDIGGSMEYTHGVGVKLAPFMLKEHGYGLEIMRGIKKMLDPN
                     NIMNPGKMAL"
BASE COUNT         5609 a         7380 c         6378 g         5699 t
ORIGIN      
        1 acgaaacgtc tcgcgagaat gcgaacttca gcatcccgga tcggctctta tcggtatccg
       61 tccagatatg ctcacgataa ctgcggtagc cgttcagttg gccgtcgcag atgggcgaat
      121 tggcgaacat tgatatgaaa atcggcgtca gccccatacc ggtgcgaaac ttcgacatcg
      181 catctttttc gtcgcggtaa tcgatattgg cttgaacagt cgcggtctgc ttcatcatgc
      241 gctggcccag cctgccgact ttgggcatat agggccccat gattcggtaa cgttttttgg
      301 gtacccattc gatttcttcc aaacgactta ccggctgcat gcccaatccg aggaaaacaa
      361 catcgagagg atcggaaacc tctaaaagct cgcggatgtg ctgggtgaat tcggcatagg
      421 tgcagtggat gctgtcgcac ggttccccgc taagctcgat ctgcccgccg ggctctagtg
      481 tgatctgcgc gttgtcgcgg ctcaacgcga taatatggcc gtcctgttct tccggctccc
      541 agccaaagcg gtcgatcagt cttttgagaa tcgactcgac gccgcgtggt ccagaataag
      601 gaatggcctt gccgctgctg cgatcgatgc caaccttttc gtactcagta ccgacgcgcc
      661 agcgttcgcg gggtttgccc gccgcatgga agtattcttc gagctggtcc tttttttcaa
      721 tgtctgacat ggttttcgat attctgtcga tacggcggat aaatgattcc cttctcggtg
      781 atgatcgccg agatcaactc ttgtgaagtc acatcaaacg ccggatttag gatattcatc
      841 ccttccggcg cgagcggtct gccgaacata tgcgacactt ccttggggtc gcgttgctcg
      901 atcggaatat ccttccccgt agggcaattc aaatcgattg aagaagtcgg tccggcgaca
      961 tagaacggga tgccgtgtcg ccgcgccaga actgcaatcg tatacgtgcc gatcttattg
     1021 gctacatcac cattggcggc gatccgatcg caaccaacga ctacggcatc gacccgacct
     1081 gtttgcatca ggtatccaga catgttgtcg gtaatcaagg ttgcggcgat cttttccttc
     1141 ttgagctccc atgccgtgag tctggcgccc tgtaaaaacg ggcgggtttc attgactaga
     1201 acttcgaatt tttgaccgga gtctttaagc gcgcgcatca cgcccagcgc cgtaccgtag
     1261 cctgcggtcg ccaatgctcc ggcattacag tgggtcatca catgttttgc atcggtcaac
     1321 agtttggccc cgaaggcgcc tatttttttg ttcgcggcga tatcttcttt atagatgttt
     1381 tgtgcttcga tcttaagcac gcgcttgaca gcttccactc cgcggtcccg gttctctaga
     1441 taaaccttct tcatgcgttc gagcgcccaa aataaattca ccgccgttgg gcgggtcttg
     1501 gccagcgttg atacaatctc attgaactct tcatcaaaat tcttggcctt actctttaaa
     1561 gctcccagcg caacccccat cgcggcagca acgccgatag caggcgcccc acggataacc
     1621 atatgggtga tcgcggcggc tacttctttg tagtcgcggc aaactcgatt gatctccttg
     1681 tgcggcagaa gccgctgatc gagcatgacc acctgatcat tcttccactc aattgttttt
     1741 acacgcatga ctacatttca attttgcgaa gaccgagtat attaggcgca agaagtaagc
     1801 gtcaagggtc actggtggat ttctatcatc tcctcggcta tctctccggc cttctcttta
     1861 tcaagcctaa catggaagca acttcgcttt accgaacgat agcgctggtt cactgtcttg
     1921 atgcgattct ttgttcggtg attgctgctc atagcggcag gcgtacggtt ggctggagca
     1981 tcggcgggct gatctttggg atatgggctt tgggtctact gtttctgttg ccgatcaaga
     2041 aacctgccgg cgcatgaact ctcgatgggc tctcccgcct gcttggtggc tccaattagc
     2101 ttcactcgtc ctgggcgatg acatacgcca tcccgtgttg atcggtatga gtgatcgaca
     2161 atgcccagtg tttgatgccg agcttctccg ctagctgcgc agttttactg tgcagcacaa
     2221 catcaggttt gccgctgtta gcgcgagcaa cttcaatatc gagccaacgc acctgcgctc
     2281 cccaaccccg acccagcgcc ttcatgacgg cttctttggc ggcgaaacgg ccggcgtaac
     2341 tttggaagcg gcctcgttgt cgcttctcgc aataagcaat ttctcgcggc gtatacaccc
     2401 gttcgcgaaa tcgaataccg atccgaggat gttccaaggc ggcgcgaatc cgttcgactt
     2461 caaccaagtc gatgccaaca ctgataatca tggattctca acctaacccc gctgctcttt
     2521 gactgtcaat cactacaagc gcgagcggag agcccaattt cgcattgact ggcgcgcgtt
     2581 gatcgcgtaa attttcccaa aaaggaggga cacacgattg tgattgcccg tcgaactcta
     2641 cttttttcat taatgctgct ctttggtatt ccgcttcccg ccacagcgca gaaaggcacg
     2701 atcaaacttg ccgttcaagc gccgctctca ggtgaacaag cggctttggg tgaacacatc
     2761 aagctcggtg cacagctggc ggttgaagaa gcgtcaaaat cattcaagtc catgggctac
     2821 gacctggtgt ttgttcccta cgatgaccaa gccaaaccgg aagtgggcgt cgccaatgca
     2881 agaaacctgg tcgccgatcc cgatgtcctt atcctggtag gccatttcaa ctctggggtc
     2941 gccctgcctg catccgaagt ctacaaagac gcgatgctgg cgatgatctc gccggccaat
     3001 acggcaactg agattaccga ccgcggctat cccaatgtca atcgcgtttg cggccgcgac
     3061 gatgtccaag gccctgtagg agcgcgcttt gccgcccagg atcttaagtt taaatcggtc
     3121 tatatcattc atgacaaaac cctgtacggg cagggagtgg cggacaattt ccgcaatgaa
     3181 gcgaagaagt tagggctcaa tgttttgggc tacgacggca ctgaagagcg cgcaaacttt
     3241 gccccgatga ttattccctt gaaagcgaaa aatccggact tggtctattt cggtggtatt
     3301 tatcatcagg gtggcttgct attaaaacag atgcgcgaaa agggcgtgaa gtcggcattc
     3361 atgggaccgg acggattgga ttcatcggaa atggttaaga tcgcgggcca gcacgtaatc
     3421 gggtcgtact atacaaccgt cgcggggccg ccggacgcct atcctgagac tgccgctttc
     3481 gccaagaagt tcaagcagcg cttcggcaaa gaagtcgaat cctttgcgat gtacgggtat
     3541 gatgcggctc aagtcggcat caaggctgtt gaacagacgc ttcaaacaaa cggcggtaaa
     3601 aaaccgagcc gtggggaagt ttctgcggca gtgcgaaagc tcgataattt cagaggcgta
     3661 accggtcaga tcagtttcga caataaaggc gatcccgtga aagccaagta cttcgtgctt
     3721 cagttcgata agaagaacta tcccggcaaa gtggtcaaag tcatcgaaca gcaggccccc
     3781 caagctaaaa agtcataact cgtccaacac tccatcacat cgaagcgaat cacgaactgg
     3841 ctcgttttcg gtactaatgc ttcatgaaaa ataagttgcg ggttgccatt ctcttcggcg
     3901 gccgatctgc cgagcacgaa atttcgctaa tttctgcccg caatatcgtc gcggcgatgg
     3961 acaagagcag gtatgagatc gtcgccatcg gcatcgacaa gcaagggcgc tggtcttacg
     4021 acagagaagc tcgtctgctc ggcgacgggc aaaaacaggt cataccgttc aatcatgcgc
     4081 aattgaccgg gatattgccg ggcgcaagcc aatcaagctt aattgcgctt ggcggtgctc
     4141 gtctgaagat cgatgtcgtg ttccccgtgc ttcacggtcc cttcggcgaa gacggcacgg
     4201 tccagggcct acttaaatta gccaacatcc cgtttgtcgg cgccagcgtc ttgggatccg
     4261 caatcggcat ggataaagat gtcatgaaac ggctgcttcg tgatgcaaga gttccgatcg
     4321 gccgatttca tgtctatggc cgtagcgccg cggatcggat taaatttacg ccgttaacaa
     4381 aggacctggg tcaaccgcta ttcgtcaagc cggccaatct cggttcgtcg gttggaatca
     4441 gcaaagtaga gaagcgctcg gaactggaag ccgcagtgag aaatgcgttt cgttttgaca
     4501 ataaaattct gatcgaagaa ttcatcgaag gccgcgaaat cgagtgttcg gtcataggca
     4561 atgacgatcc gatcgcgtcg ctcccgggag aaatcgtaac cggtcataac ttttactcct
     4621 atgatgcaaa gtatatcgac gatcagggat cgcgcctgga gatccccgcc aaattaccga
     4681 gagcaatcgt caaatccgtc cgggagattg ctatcaaaac ttaccgaaca ctatgctgcg
     4741 aaggaatggc ccgggtcgat tttttcgtta aggctaacgg tcaggttctg gtcaacgaga
     4801 tcaacacgat ccctggtttc acccagataa gtatgtatcc gaagatgtgg gcagcaagcg
     4861 ggatttctta ccccaaactg atcgaccggc tcattcagct tgctctgcag cgtttccgtc
     4921 aggagcaaaa acttcggacc agcgtttagt tacccgacca gagcaataaa actcctcaaa
     4981 gcccggacag ccgcgcagct gatcgagacc acgtctatga actggcggtt tacctcttag
     5041 accggcagca ccataagaac cggccatttc aggtgtcgga cgctaccatt gcaacgtcgc
     5101 gacgcgcgcc gcccaaccgt cccactcgag ccgacaccgc accttgatcg gacgaatctt
     5161 cgattcgccg agcggaaccg caccacagca aactaggact tattttctaa gggtcctgcg
     5221 aacgaacccg tttagtccat ctgccgtctc aagaaagtcg gaatgtcata cttgtcatct
     5281 tcctcctgcc cggtgctcaa ctgcaaggtg cttttgctga gcgtcaccgt ttccacttcc
     5341 tcgctgccag gttttttgcg ctgccaggtt ggcgcgtcga gatcgtcaac gatggtcccg
     5401 agatgcacaa ccttcttagt cttgaccggg ttgacgtttg ccacgttagg cacgctgctg
     5461 gtgacgtgat tcatgctatt cacgttgttc atcgaagaga cgtgaccgga gcttgtgatc
     5521 accggtttgc gctcgtcctt gccttctccg aaaccggttg caatgaccgt gatgcggatt
     5581 tcatcgagca tggtttcgtc tataaccgca ccgaaaatga tgttcgcctc gtcgtgagcc
     5641 tcttcctgaa tcattgaagc cgcctcgttc acctcatgca aacagagatc gggcccgccg
     5701 gtgatgttga tcagaacgcc gcgcgcgcct tggatcgaaa tgtcttcgag aaggggactc
     5761 gatattgccc tctgcgccgc ttcgatagcg cgattctccc cggtcgcagt cgaagcgccc
     5821 atcaatgcca agcccatctc cgccatcacc gttctgacat cggcgaaatc aaggttgatc
     5881 aagcccggcg tgataatcag atcagagatc cctctgacgg cttggagcag cacttcatcc
     5941 gctttgtgaa acgcttctaa catcgttgtc gttttggcgg cgatgctcag taatctctga
     6001 ttcggaataa cgatcagggt atcgacgctg ctcttgagct cggcgatgcc ttcttctgcc
     6061 tgccgcatcc gtttcttgcc ttcgaagatg aacggcttcg tcaccacgcc cacggtcaaa
     6121 gcgcccactt cacgggcaac cctggcaatg accggcgcac ctccggttcc cgttccacca
     6181 cccatgccgg cggtaatgaa aatcatgtcg gaaccggcca ggtactcttt gatcgtttcc
     6241 tggtcttcca aagccgcacg ccgtccaatt tccggattag ccccggctcc aagtccctta
     6301 gtcaccgccc caccgagctg aattctaacc ggcgctcgac tggcatccag ggattgcgcg
     6361 tcggtgttgg ctacgagaaa ttcaacaccg ttcagcttac cgccgatcat tgtattcacc
     6421 gcatttccgc cacccccgcc gataccgata accttgatac gggcgttcag attgaactcc
     6481 tcgacaaatt caaacataag ctttcctcct tatattacgg ttaataggaa ctgtttgtgt
     6541 ttagaaaaaa tcacttaacc aatccgacat tcgatgtttt accttcgaca ggatggtcgc
     6601 atcctctcgc acacgaaaaa aactgcgctc ttggcgcttg atcccataga gaaccaaacc
     6661 gatggcgctg gaataaactg gatttgtgat cacgtcgacc aaaccgccga cgtgccttgg
     6721 tgtgcccagc cgtaccggac agccgatggc gagttcggcc gcttcgatgg cgcccggcaa
     6781 caacatcgaa ccgccggtca tcaccacacc ggagccgaga tagtcttcgt agcctgactt
     6841 gacgatctcc cggcggatca actgaaagac ctcgtccatt cgaggttcaa taatctcgca
     6901 caagatttgc cgcgagacgg agcgttgcgg cttaccggca gaactaggaa cttctaatcg
     6961 cacctgttca gcgaccatgg atgccatagc acaaccgtaa cgaagcttga tccgctccgc
     7021 atctgcgaaa ggcgttctaa gaccggcggc gatatcgttg gtcacatgat tcccgccgat
     7081 cggcagaaca gccgtgtgct tgaccgtgcc gtcgtgaaaa atcgtaatat cagtcgtgcc
     7141 acttcccata tccgccaacg ccacgccgag atctttttcg tcctcggtga gtaccgcatc
     7201 ggcgcacgcc accggcatca gtgcgacatc ggcaacatga agcccggagc ggttgcagca
     7261 tttaatgaca ttttgcatgg cggtagtcgc tccggtaaca acgtgaactc tggcttcgag
     7321 tctgaccccg gacatgccaa gcggatcttt gatcccgtct tgatcgtcga tgatgtagtc
     7381 ttgaggcaga acatgaacga tctcctggtc agttggaatc gccaccgctt tggccgcgtc
     7441 caagacccgg tccaggtctc tctgactgac ctccctagtc ttgaccgcga caacgccgtg
     7501 gctattgaag gctttaatgt gacttccagc gatcccggta aatacagtgt tgatttcaca
     7561 gccggccatt aacgctgctt cttcaacagc cttcttaatg gatgccaccg tgttgtcgat
     7621 atggatgaca actcccttgc gcattcccag cgagctatga gagcctatcc caataatttc
     7681 cacgccatgc tcggtcattt caccgacggc gctgcagacc ttggacgtgc cgatatccaa
     7741 tcccacgata atgctgtgtt tcttcgccat acctgtgttg ccgaattgtc tgtttcttat
     7801 tgcggctggc gccgcaaccg ggttaccact cgatcttgaa aacttatatc cagagagccg
     7861 agtctctcct cttttcctcg ccacaatgtc atcacgcgat ctaggcgttg gagcttccct
     7921 tcccaatcgc cccaccccat gtgaatggcc accggatgag cgatcgtata gaccaccaac
     7981 cggtccggcg catcgaagtg gatttccgac agagcgtgcg aatccttagc catcagatcg
     8041 ccgaggcgca aggcgtcctg gatccttcgc cgcaccgcgg gatcgaagga tccgagttct
     8101 tcggtccgaa tgccggtcag catggggaat ccgacatttt ctccttgcgc tacctcttta
     8161 aagagaaccc cgtcggcatc gacatagtat agtttgccga cggcgacgat tgccttgggt
     8221 cgacgctcct ccacttcgat gacaacacgg cgcgggaatt cgcgacgaac cagcactcgc
     8281 cgcacccagg gatgctgcga aaccttcttc tctatttttg ccgggtcgat gctccaaaga
     8341 tttatcccat gccgcaatcc ggccatcgaa ataatctcgt tgccgcgcac cttgtcgctg
     8401 cctcggactt ggatttcgcc cacggcgaag taaggattct cgaaaataaa gcggcgcact
     8461 gccaccgacg ccaaaccgac ctgttcgcgc catccataga cagtccaagc ggcaacgacg
     8521 aacatgatca tgatcccagc caccatcaat cggcggcgcc actggaattt atcttttttc
     8581 cggttgtctt ttcgagaaac tgccaactgc atcgcgctta ctccactcta agcgacggcc
     8641 acctgccgat cagcttcact tcgggaatta agaccacgcc gaactttgct tgaacttcag
     8701 cacgcgccag ctccataagt cgacgcacgt cttcagcgct ggcgccgccg agattgacta
     8761 tgaagttgcc gtgcttcggc gaaatttgcg cgcggccgat ctgctttccc ttgagcccgg
     8821 cagcctcgac taatctcccg gcgtagtcgt ccggcggatt gcgaaacatc gagcccgaat
     8881 tcgggtaacc ggaaggttgg ctgctcttcc gtttgatgac cagctcacga actttgatgc
     8941 tgactctgga cgattcttgc cgatgcaggg acaagcgcac gcgggtgaca acgattcccg
     9001 ctggcaaatg cgaatcacgg taagtaaatc tcatctcttc tcggttaagt tgaatcggag
     9061 ttccttggcg agtcatgccg taaacagcga cgaccacctt ctcgaattcc gacccgtacg
     9121 ctccggcgtt catgaccagc gccccgccga cgctgcccgg gattccctcg gcaaactcca
     9181 gacccccgta accgcgccgg gccgcctgcc gcaccagctg cgtcacggcc tgtcccgcgc
     9241 cggcatctac ccatgactcg tttgcttgtt cgtgccattc ggcattcttg aaagcgccga
     9301 tcagatgaat cagcacgccc tcgataccta gatcgctgat caagacattg ctgccgttgc
     9361 ccaatagccg gaacggcatg ccgcgcaagc tcaacaaaca gagcagttgt gccaacgcgg
     9421 gttcgctttc tacctcgacg aagtaatccg ccgggccgcc cactttcatc gacgtgtaac
     9481 gcgccagcgg ttctgaaact ttcaccctca gtccggaaat cgactggagc tcccgcacca
     9541 ggtttgtttc gttaggcttc ggattcattg ccaacattag aagagctttt cgatgcgttg
     9601 cagcattaaa tcttgaacag ctcctggacg catgaatgat ccgaaggcgc tactcgcggt
     9661 tcatgccaag gctgcaagga tgctttctcc aactttatag atatctccgg ctcccaaagt
     9721 caggacgaca tccccggcaa tcaatcgctc ggccaattgc ggcgcaatgc tcgtcttatc
     9781 ggcgatgaat tcaacatcca tatgtccctg gcgcttgatt gcctggtaga gcgcttcgcc
     9841 ggtaacgccg gggacgggat cttctccagc cccatatatt tcggtcagca ccaaacgatc
     9901 ggcgccttca aacgctgtca gaaaatcgtc aaagagatcc tgagtgcggc tgtatcggtg
     9961 cggctgaaag atgacggtca acggccgttt ccaactttcc cggatggcgc cgatcgtcgc
    10021 gcggacttcc accggatgat gaccgtaatc gtcgatgatc aagacatcac gagtttgccc
    10081 ctttatttcg aagcgtctat ggatgccgtt gaacgccgca agcgcctccg cggtgcggca
    10141 aaaggggatc tccagctcat gagcgacggc gacggcggcc agcgcattgg tcgcgctgta
    10201 gcggccggga agctgcaact tcagctgccc aaggggattg ccgtgatgca gtaccgtaaa
    10261 ttccacgccg cccgctttca tgtgcagatc ctgaacggaa aaatccgctt ccggagatag
    10321 accgtaggtg gcaaaccgtt tgcgcacttt gggcaacagg gcacggacat tgggattgtc
    10381 gatacacaac actgccaaac cgtaaaaagg aagcttgttg acaaatgtta ggaaactttc
    10441 attcaagcgt tccatcgtct ggtgaaaatc catatgctcg cgatcgatat tggtgacgac
    10501 cgcgatggtc ggcgtcagca gcaagaaact gccgtcgctc tcatcggctt cggcaaccag
    10561 gatctcgccc tgccccatct tggcattagt gccgagcata tgaacccggc cgccgattac
    10621 cattgtcggg tcgaggcccg cgagacttag aaccgcagcc gtcatcgacg tcgtggtcgt
    10681 tttgccgtgg ctgccggcca cggcaatgcc gtacttcatg cgcatcaact cggcgagcat
    10741 ttccgctctg gggatgaccg ggatctgccg ccggcgcgcc tccagcactt ccgggttcga
    10801 gtacttaacc gccgtcgaga tcaccactac tgacggattc acggtcagat tgtcttcctg
    10861 atggccgaca aaaatctgcg cgccaagctt gcgcagccgc cgcgtcgcgt cgctgtcatg
    10921 cagatccgaa ccgctcacca cgtatcctga gttcaagagc acctcggcga taccgctcat
    10981 cccgataccg ccaatcccga caaagtgaac tcgatgtttt ttatgcaaca tgaacgactg
    11041 atcactcttt cgcctcaggt atgcgccagc gcgtaacatt catcgacgat gcgcgccgcg
    11101 gcctctggct tgcccatcgc gcgcgccgcc gtggccatcg gctcgatttt tgtcggatcc
    11161 gctacgtaag cccgcagacg agactccagt gctgcgccat tcaattcgtg atcgagaatc
    11221 atctcggcag ctcctttgtc ggcaagcgct tgagcattcc accgttggtg atcgtagatc
    11281 gcgtacgggt acggaattag aatggcggcc ttgccgaacg ccgtgagctc cgctactgtc
    11341 gtagcgcctg ctcggcacag cacgagatcg gcgcgcgcat aagcatcgtc catgcggtcg
    11401 ataaacggca gcgcctccgc gtcaaagggt aaatcttggt aggctttgcc aatggcagga
    11461 aaatccgccg cgccggtctg gtgaataatg cgaaactgcg aggccaaatc aatcatttgc
    11521 ttgagcgcgt caattaccgc gatattgatg cggtgcgccc cggcgctgcc gccgaagatc
    11581 aacagggtga acttttcacc tttgggcgcg ttcggcagcc tgtgccagcg aactggagtt
    11641 ccggtctcca gcaccttttg ggccggcaag taggaagcgg tttcgcggta agaggtaaaa
    11701 attttatcga caaagcgcgc cagcatcttg ttggtaaagc ccggttgaag gttttgttcc
    11761 atgatagcgc agcggatgcc ccgcaacttc gccgccacta gcagcggtcc ggatgcatag
    11821 ccgccgaggc cgagaatgca atccggtcga aacctcttga ggatacccag cgattgcagc
    11881 agccccgcgg ggatcccgta gagggcgtcc cataggccgc gaaccccccg acctttaagt
    11941 cccttcacag cgatcagctc gagagggaag ccctcacgcg gcagcacccg cgcttcaatc
    12001 cctttatcgg tgccgacaaa caagatttcc gtcattgcgt cacgtcgttg aaactcgcgg
    12061 gctacggcca gtcccgggaa cagatgacca ccggttcccc cgccggccag aatcacccgc
    12121 atctataatc tcctgcgcga gagccctaac aataagccta cctccgccag attgacgaca
    12181 agggccgatc cgccgtaact gataaacggc aaaaccaggc ccttggtcgg cataagcccc
    12241 atgaccacgc ccatgtgaac taaaccctgc atccccaaca ggacggttaa gccgaaagcc
    12301 agatactgct cgaacggctc ctcgattttc gccgtcaagc gcaaaccgcg catgaggata
    12361 acgccgaaga gagccaacac agccagggcg ccgagcaaac ccatctcctc gccaatgact
    12421 gagtacacga aatcggtatg cgcctccggt agataaaaga gtttttgccg gctctcccct
    12481 aatccccggc cccacagttg accggaaccg aaggcgatga gcgattgaat aacttgaaaa
    12541 ccgctgctgg atgcgtcgcg ccaaggatcc ataaacgtca tcaagcggcg caaacggtat
    12601 tccgcggtca acatcacata agccaacaca ggaagcgcca acgcgccggt gcccaagaga
    12661 tgcgggatgc gtgcgccgcc gacaaacaac atcaagtaaa gcaagaccgc aagaatcaaa
    12721 gccgtgccga aatccggctg taacaacaga acgccgagaa cagcgccggc gacaatcaga
    12781 tgaggcaaaa caccgactga gaaggttttg atcttttctt gcttgcgcgc cattgaatgc
    12841 gccagataaa agaccaaggc gagctttgcc aactcgccgg gctgaaatgc gaagccgggg
    12901 agcatcaacc accggcgggc gccgccgcgc atgaggccga tacctgggat caaaaccaga
    12961 accaatacaa caagagtcac tgccagcaaa ggataggcaa ggcggcgcaa caacgccggc
    13021 gggatcagcg acccgatcac taagccaacc aggcccgcgc cggcggcgat cgactgcttg
    13081 cgaaaaaaat aaatgccgtc gccaaaccgc tcctgagcat aaagatagct ggtgctgaga
    13141 accatggtca gccccaaagc cgttagcgcg acaaccgaaa agaaaatcca tttatcgacc
    13201 gccagcgcat ctctcataag tctcgcacca agcttttgaa gactttgccc cgttcggcat
    13261 agttgcgaaa catatcgaag ctcgaacacg ccggcgacaa caacaccaca tcaccgggcc
    13321 gcgcatgcgc ggcggcgtcg gccactgccg cggcgagatc ttttacgatc acggtttcgg
    13381 tgaggtggcc gagcgcttcg gcgataacgt ctttggctgc tccgaacaaa accaagcgcc
    13441 gcactcgttg ccgcatgcag ttttgcaagg ggccgtaatc gccgccttta tcgacgccgc
    13501 cggcaatgag aatcaccggc tcggaaaaac tcgccagcga cttttctacc gcgccgacgt
    13561 tcgtcccttt ggagtcgttg aagtagcgaa cgccatcttt ctcgcggaca aactcgagtc
    13621 gatgctcaag gccgggaaaa ctctccaaag tctcttggat gacgtcatgc ccgacaccga
    13681 ccgccttggt cgcagcgatt gcggccatca tgttctcgac gttgtgaacg cccttgatct
    13741 taactgccgt cagcgaaaat gtttcttcgt ccggcccgtc gcgccaaacg atcgcatcac
    13801 gttcggcaaa cacaccgaat ccaacttccg acgagccgat cgagacaatt tgcgaacgca
    13861 attgccgccg catggcccaa acccgcgaat cgtcgcggtt gagaatggca atgtctggtt
    13921 catcttgcgc ggcgaagatt cgttgtttgg cactggcata agcggcaaaa tccgcgtaac
    13981 ggtcgagatg gtcttcgctt agattgagaa gcacggcgat gcgcggacga aaccatttca
    14041 cccattcaag ctgaaagctg ctgacttcca caactcccca ctcccagtta tccgcgccgc
    14101 atccgatcag aggcatgccg agattgccgc cgacaaaagt ttttgttcca tttgccttaa
    14161 gaatctcgcc gatcagtgtc gtcgtcgtgc tttttccatt cgttcccgtg acagcaatca
    14221 gaggcagcgg gagaaagcga taagccaatt cgatctcgct gatgatttcg acgccgcgct
    14281 ggccggcgtt ctcgagcagg aaattgttca ttggcacccc ggggctcggc acaaccatgt
    14341 caacgccttg caaccaatct acatcctcgc cgccgaggtg atattcaatg ggcaaacccg
    14401 caagcgcgtt cacctcggct tgcagatcag ccgccgggcg aagatcgcta accagcacga
    14461 ccgccccttg ctgcgcgaga aaccgcgccg tagccaggcc ggtgcgcgcc aggcccaaaa
    14521 ccatgacttt cttgcccctg agatccattg ttgggtgcca ttttacctga gcttgagcgt
    14581 gctaaggccg attacggcgc aaataaacgc gataatccaa aaacgcacga taatttgcgg
    14641 ctccttccaa cccttgagct cgtaatgatg atgaatgggc gccatcagaa agactcgctt
    14701 gcgtctgagc ttgtaggagg cgacttggaa gatcacggaa agcgcctcca cgacaaagac
    14761 gccgccgacg ataatcaata gaatctcgtt cttggtcatc acagcgacgg cgccgagagc
    14821 cgctccaagc gcgagcgagc ccacatcgcc cataaacatt tgcgcggggt aggcgttata
    14881 ccagagaaat cccaaacctg cggcgaccac tgcggcgcaa aatacgctga gctcacccac
    14941 gccagcgaca taagggattt gcagatagtc ggccaacttc atatgtccgg taacataggc
    15001 gatgaccgcg taggtgccgg cggcgaccat taccggcccg atcgccaatc cgtcgagccc
    15061 gtcggtgaga ttgaccgcgt tggaagcgcc gacgatcaca aacatcgcga aaggcacata
    15121 gagtatgccc agatcgggac gaaaattctt gatgaaaggc agactgatct cggtgctgaa
    15181 cttaggcata tagtaaagcg caacggcgac aacaaaggcc atcaagaatt gacccagcaa
    15241 tttcgcccgt ggcggcaatc ctttgctgtt cttgcgagta agcttcaggt agtcgtccac
    15301 gaatcccacc agtccggcgc ccacggtcgc aagcaacgca agcaacacgt agcggttgcc
    15361 gatatctccc aacagaagcg ttgaaaaggt tagcgccagg agtatcagcg cgccgcccat
    15421 ggtcggcgtt ccagccttga ctgcatgcga ggccggcccg tcatcccgga tgtgctggcc
    15481 gatctgcttt tgtgttaacg ctcggatcaa acgcggtccc aatagaaacg atataagcaa
    15541 cgccaccagc gcggccatcg ccgcccggaa agttatgtag cgaaaaacat tgaagccgga
    15601 atatgtcgtg tgcaatggga acagcaggta gtaaagcaag ttcaatctag cctttcagct
    15661 cggttaaaac tgtttccatt ttcatacccc gcgacccctt gatcaaaagc caatcgcctt
    15721 ttttcaagtg ggcacgcagc cgctttccaa tgtcgctgtg actttcgccg acgataactt
    15781 gatccgactt cataccgccg cgtaatgcac cctttctgac gtccggcgcc cgctcgccta
    15841 gaaggtaagc gcgatccagt cgggcctgcg ccagctggtt gccgagacgc cggtgctcgc
    15901 gttggctttg tttgcccagt tcgaacatgt cgccaagcac ggcaatccgt tggccacggg
    15961 aagagatttg ggccagcgtt ttgatggcgg cgatcatcga tgccggattc gcattatagg
    16021 catcgttgat gatgccgatg ccgcgccagt tctccagctg cattcgcatg ctataaggct
    16081 tgacccttgc caaaccgcgc cgaatcatcg gcagaggtat gcctaagctg taggccatgg
    16141 cagaggcgcc gacggcattg gcaatattat gctggccgac gaaattaagc cgaatctgcg
    16201 cgcgctgatg cccggcgcgc agagtaaatc gcatcgcgcc ggaatccagc aataaaccgg
    16261 attccgcgcg aatctgacca ccctcaccgt aagtgatttt ttcgccgctg aatcttgcac
    16321 ccaggcgccg cacccgggga tcatcgaggt taactacgat agttccgccc gcgcgcatgc
    16381 cgccgaacaa cgcgcccttt tccttggcga ctccggccaa actgctcagc ccttccaaat
    16441 gcgccggcgc caccgaggta ataagcgcca catcgggcgc ggcgatttca gccaagcgtt
    16501 gaatctcgcc ggggcgattg gttcccattt ccacaactac aacccggtgc cttttgcgaa
    16561 gccgaagcaa ggtcaacggc aagcccacca gattattgaa attgccttcg gtggttaaaa
    16621 gccttccctg gagcggttgg ccatccaacg atgcgcactg caaaatggcc gctagcattt
    16681 ccttggttgt ggtctttccg ttcgagccgg taatcgccag caccttgggt cggtattgtt
    16741 cgcgacggaa gtgcgccaga tctcccaggg ctcggagggt atcgggcact ttgacggccg
    16801 taacacttct gagatcgacc gcgcgaagag cttggtgaac cacgagacag ccagctcccc
    16861 ggctgattgc atctttgacg aagcgatggc catcgagcct ctcgccctta agcgcgataa
    16921 aaatagagcc tgttttcact ttactggaat ccgtgacgat ctcatcgaac cggctggcgc
    16981 ttgctcgttt ttccaatctg ccgccggttg ccatgagaat ttcttccagc gtccatgcca
    17041 tactcaagcc cgcaaggcgc ccgcctgaga acttaaaatc tcccgagcta cttcgcggtc
    17101 atcgaaatgg atcttgtgcg ctccaagaat ctgataatcc tcatgccctt ttccggcaat
    17161 cacgaccaca tctcccgccc gcgcccaggt caatgctagg ccaatcgctt tgcggcggtc
    17221 cgcctcgacg ccatacccgt gaacaccgtc aacttttgtt ccttgagttt gaagtctgac
    17281 aataccggtc ttctgcatac ccgcctcgat ttcctggaga atgctttccg ggttctctga
    17341 acgcggattg tcggatgtca aaacgacgat atcactgaga cgtgccgcaa tttcgcccat
    17401 caaaggccgc ttgccgcgat cgcggtcgcc gccgcaacca aaaacagtta aaacttttgc
    17461 atcggtcaga ggccggactg cgacaagagt tttctccaac gcatccggcg tatgagcata
    17521 gtccaccaga accacaaccc cgcgcgagtt ctcgacccgt tgcaagcgcc cgggaacgga
    17581 tcgcaacttc ttgatgccgt cgacaatcgc cgcctgcggg attcgcaacg caacaccgac
    17641 acccacggca gcgagaatgt tatcgaagtt tgcggcgccg atgagctccg atgaaaattc
    17701 gatcactcga tctttgacct tgatttttcc actctgaccg gcggcgctgt gatgcacctg
    17761 cagcggtttg atatcgcact cgtcgctgga gccatagctc caggtggtta tcccggcgcg
    17821 ttgcgctcta ccgataagct cggatcctct gggatcgtcg ccgttgatga cggccgcttt
    17881 cgctgatttg ccgcttgctg ccaggtaatc ggtaaacaac ctgctcttgg cgacgaagta
    17941 ctcatccata ctcttatgat aatccagatg atcgcgtgaa agattggtaa agacaccgac
    18001 gtcgaagtcg atgccgcgaa cccgttcctg gaccaacgca tgggacgaaa cttccatggc
    18061 cagcgaccgg gcaccttgat gcgccatttc ggccatcaga gcatgaacat ctagagattc
    18121 tggtgtcgtg tgatgcgcag gcatttcttt gccgccatag cgatagttga tcgtcccgat
    18181 cactcctggc tccatgcccg ccgaagcaag tatggactcg atcaggtagg tggtcgtggt
    18241 cttcccatta gtcccggtaa ctcccacgat ctttagacgg cgccccgggt gcccgtaaaa
    18301 ctgggcggcc cataaaccca tcgccgctct cacgtcgtgg acacgagcga acgtcactcc
    18361 tagaggtact ttggctttat cggcaacaac gacggcgatg gcgccgcgtt cgatggcttg
    18421 atttaagaat tgatggccat ccgcctgctc gcccggcacg gcgaaaaaca cgtcgccttt
    18481 ggccacccgc cgcgaatcgt aaaccagacc tgctacttct tgatcaagat ttccttcggc
    18541 ttgcgcgagg ttctgcagcg tcagaaaatc tcttagacgc atctcggatc atccttgcag
    18601 gttcaacacc agcatgccgt tttcatccca gcgtcccccg ggacctggtg attgcttcac
    18661 cacatagcca ttgccatgga tttttacttt gacatttaga tcacgcgctt tctccacggc
    18721 ttcgcgcaaa ctgagaccaa cgaaatccgg ggttgctccg ctatcgacag cagcctcgcc
    18781 gttcctcctc ggcacgcgcc gcagcggaat gtccgcctcc agcaggggag cggcaatcgg
    18841 ttccgcggtc ttggcggcaa tgccaaaatg acgcaatgcg ccgcgcgcga tattctgaaa
    18901 tgccggagcg gctacaacac cgccgtagac gttactttgc ggctcatcga ccagaaccat
    18961 caacacaagg cgaggttcgt ctgcaggaac gaacccgacg aaggatgcga cgcgtttttt
    19021 ggcatcatag ccgccattga ccagatccac cttttgcgca gtgcccgtct taccggcaac
    19081 attaaagcct tccactttcg ccatcgttcc ggtgccgccc tcggtcgtaa cctcttggag
    19141 catggaggcc agcgaacgag cggttctttc tgaaatcaca cggcgaacca tatgaggctg
    19201 attttccacc aagacctcgc ccttaggact cagcgcccgc cgaacgacat agggtcgcat
    19261 cagaaatccg ccgttggcga tagccgcata ggccatcacc atttgcagcg gcgtcgctga
    19321 aagcccttga ccgaaggcat gggtcgccaa gtcgatcgct gaccatgttt ccggtcgccg
    19381 cagtaatcct ggagcttccc ccggcatatc aatgccggtc acccgcccga aaccaaactt
    19441 ctcgatgtat ttgaagaaac gatctttttt caatttgtcg gcaaccttgg aaaagccgat
    19501 gttgctcgac acctgaagga tcttggagaa tggcagccaa ccatgcggat ggctatcgtg
    19561 aatgattctg ccggcataac tgaatttgcc gtattcacaa aaaaacaaat cctcttgttc
    19621 gacgattccc tcttcgagcg cggccgctgc gaggattgtc ttgaaggtgg aacccggttc
    19681 aaagctgtcc gtcaccgcac gattgcgccg ctgctcaggc gaggcgctcg aataattatt
    19741 tgggttgaac gtggggtaat tggccaatgc caaaatttca ccggtgcgcg gctcgacggc
    19801 aatcgcaacg cctgccttcg cgcggtattt atcgatggag gcttccagct ctttttctgc
    19861 cagatgctga acggacgtat cgagcgttag gtgaatgtcg ccgcccgcgg ggatttgcaa
    19921 cccttcgacg ccttcgacca gcacacggcg gcccaaggca tctcgctcgg atacagacga
    19981 acccgtttca ccacgaatat attcattgta gcgcagctcc aaaccttcga gcccttcgga
    20041 gtcacgacca acaaagccga tcacctgccc ggcaagctga ccctggggat aatagcgatg
    20101 cggttcgtga aacatgccta cgccttcgat cttgagattc tgaatttgtc ccgcctcatt
    20161 gggcgaaatc tgccgcttga tccaaacaaa aggcttgtcc gacgtaagcc tctctttgat
    20221 ttccgccgtg ctgattttca agattcgcgc tagatcacgg gcgctttttg taggatcctg
    20281 aatccgccca ggccgtgcgt aaaccgactg cgcctcgaga ctcaacgcca gcgcttcgcc
    20341 tccccgatcg tacacgccac ctcgctttgg cagcacggtc gattctttca gatgttggcg
    20401 ctgtcccaag cgcatcagcc tctcgccctg caacgcctgc agatagaatg cgcgcccgcc
    20461 gatcaccagg aacatcgcca gaaaaagaat cttagcgaca gccaagcgaa catggctggt
    20521 ccgcggagtg cgtctcatgg cagtacgata acctgaccct tctcgggagg caccaacccc
    20581 agccggcggc gcgccatcgc ttccaaacga tctggggagg tcaagctggc cagttcaacc
    20641 ttcaactctc ggttttcttg ttcaagccga ccttgcagtt ttgtggtcgt tgaaagcacg
    20701 tagcccatgt gaactacctg taaacgcaac cacacatgaa ccaatcctaa aatgaacaga
    20761 caaaagccca gagcgcagat gggaatgagg tgccgctggc ttttgcgctt cggagaccgg
    20821 ttaggctgct tcgctttaac cgacctaacg gggcggggtg aatttagatt ggcggcgctg
    20881 gccgtttttg ttctcatagt cgcactgccg cgcgcagctt cgccgcacgc gcccgagggt
    20941 tggccctgac ttcttcagcc gatgcgataa ttggtttgcg ggttagtctt tgcgccttct
    21001 ggctccagcc gcactcgcat ttgagagttc tgggcgggca aatacactta tcgctccatt
    21061 tgcgaaaggc tgtttttacc agtcggtctt ccagtgaatg aaatgaaatg atcaccatac
    21121 gccccccagg ctggagcaat tcaaagccgt attccaagaa ctgcttgaga gattccattt
    21181 cccggttcac cgcaattctt agcgcctgaa aggtccgcgt tgccgggtga tgagcgcggt
    21241 cccgctttct cttcactcgc gcgacgaggt ctgccagatc gagcgtagtc tcgatcctcc
    21301 gctccttccg ctctgtcgcg atcgccgcag cgatgcgacg cgcccggggc tcttcgccat
    21361 agtcgctgat gatcttggtt acttccgcct caggaaaaga attgacgatt tcagaagctg
    21421 tcaatcgctg acgacggtcc atgcgcatgt ccaatggagc gctcgcttgg aagctaaaac
    21481 ccctttccgg cgtatcgaac tggtgcgatg aaacgcctaa atccaagagc acaccattta
    21541 cttgattcca atttatatcg gctagaattt ccttcgcctg atcaaaattg gcctggcgaa
    21601 gtgtcagccg atctccgaag cgactcaatc gctgcgctgc tgcctgcaac gcatcctcat
    21661 ccctgtcgag gcctaagact cggccgtcgg gactgctgcg aagaagtagc tcctctgcat
    21721 gcccgccccc gcctaaagtt ccatctagat aacgttctcc tgctctgggc ttgagcagcc
    21781 ccatcgattc ctgaaccaga accgaagtgt gcgcaaactt cattaatgac tagtgccgtc
    21841 accctcccaa tcgtcatcga agctctcgat cgcgtcttca agagaggtat aatcttcgaa
    21901 aaaacggtcc atgccgacaa ctttgaagat atcgcggaga aaaggactca tgccggcaag
    21961 cttaagatca ccattaagat ttctgagtag tttcaatctc tcgagcagga ccccgatact
    22021 caaatagttt atgtgatcga ccgcccggag attaagaacc accttcaggc ggtccttctc
    22081 gatcagtccg ccgataacgt ttttaatgcg gatcatttcg cgaaaatcga tatcgccgga
    22141 aacgtcaatc acggagatat cctgaatgtt attttgcggt agcatttcct attctccttt
    22201 catccagcca cggcgataaa tccgcccagc attctcacaa ccccaggtcg ccgagaaaat
    22261 caggatcttg catcaatttg ccttccgcat cggcgaagaa ctgcttccag gtttcttgat
    22321 cccagacacg aaatttttcg agagctgaaa ccaaaacaac gtcgcgcttg aggtcggcgt
    22381 acttccttaa agaggtaggg atgagaatgc gtccctgttt gtctaaaagg cattcacagg
    22441 ccccgcctaa gtaaaagttt tggaacaaca ccatccgacg ttcgaacttc ggccgctggc
    22501 taatctgctc ttcgagccgt aaccattcgt cgatgggata aacgtcgaga cagcgcaagc
    22561 cgtccacgac gaagttcgtc ataatgatcc gttcatcgcc cttgccgagc aaaatatccc
    22621 ggaatttgga cgggatactt agccgtccct tgccgtcaag agtgtgctca aagctgccgc
    22681 gaaacatatt aagagatcgg ctagaaagct gacttatggg aaattatccc actttctccc
    22741 acctctgcgc cacttataac ttcacgacta cgcgctgtca ataaatttgt taaatttttc
    22801 cagctttttt aagtttatac atccggtttc gtagatcgcg gcgacgccct ataacacgcg
    22861 tgacaagaca atctcgtgcg cttacggctt taaaagaagt ttggcagtgg gatgtcggcc
    22921 cacgaggcga gctagtttga caaaaacagg ggtgcgatac gccggacaga aggagtatga
    22981 gctaaagtgg atcgataagc cggattctgt aatgactctc agaaggagtc atagcgatca
    23041 ttcatcttgc cccaggattg ctcctggggt cgagcgacct tacccgaggg ttatgggcgg
    23101 accaccctac cctcctattt ggtcttgctc caggtggggt ttgccatgcg tccatcatca
    23161 ctgatgaacc ggtgagctct tacctcgcct tttcaacctt accttgccgg gacggcaggc
    23221 ggtatatttt ctgtggtact ttcccccgat cactcggagc cgccgttaac ggccaccttg
    23281 tcctgcggag tccggacttt cctctcctca gactacagaa tcgtgaggag cgatcgctta
    23341 atccacttta gcccgtctga gattgctgaa ggcctcatgg tatctcgatg agccgacagc
    23401 ccgctttgtt cgcttcaggc atacacgaaa agagacgata gcgtccagcc gacgaagaaa
    23461 caatacatgg attcttttgg cttcgatggc agacgggggc attatcagct gacccatgga
    23521 gtctggcctg agcaaccaag acagatacac atgttatctc tcgggagcga tctatggtgc
    23581 aatggcgtag agcccgaaac gacttattag gcggtcttct cgtgctcttc cggggccttg
    23641 tagtgcagga tgcgctgaca gctaggacaa aggttgacct tttcgctccg aatgatctca
    23701 ttccagagct gcggcgggat gttcatataa caaccttgac aaataccgct catagcttcc
    23761 acgaccgcat tgccgccgcg gcgcgaaaag atcaactcat aacgcgacat taggctggcg
    23821 tcgatttctt cggcgatgcg ctgccggctg actgaggctt tgttcaccgc ctcatcaatc
    23881 gcggcgacct gagcttgcaa cgcttgctgt ttttgttgcc aatcctgctg ggcttcaagg
    23941 agcgtttctt gtttggtttt aatctctccc ttgaagccat caatctctac catcatgttt
    24001 agcagttctt cttccacgtc accgtttgcc tgccggatct gatcaatctc ccgctgtaaa
    24061 gcctgtagtt ctttaacgtt tttgatgcga ttcatccgca tccgcttgtc cgttgccttt
    24121 tggctttcct cgtgcagcag tttatccttt tcttgccgaa gcttttcctt tgccgcgcac
    24181 gcggccgtca gggtttcgat ttcttgtttt ttttcatgaa tgaatcgctg tttttcctgg
    24241 agttcgctca gtagcccttg tttgacggcg gcgtgttctc ttatttctcg atcgataatc
    24301 tgtaatgcag ccaaaatctc aatttgtgcg cgcaacttct tctccatcaa tcaacaaaaa
    24361 aaatgcaccc ggttgggtgc agttgttaaa atgcctgttt gtgacttgtg cgttttgaag
    24421 gctggtggtt ctcaatcctc gtctcaactc cacatctaat atttatattg atttttttat
    24481 gttgtcaaag cgactcgacc cctggctgtc acgtcggacc catgagcccg aacagggtga
    24541 tgggctcaca aattagaggg ccatctttcc gggattcatg atgttattgg gatcgagcat
    24601 ttttttaatg ccgcgcatga tttctaaacc gtaaccgtgt tctttcaaca tgaaaggcgc
    24661 aagtttcact cccaccccat gagtgtactc catggaaccg ccgatgtcct gtgccaattt
    24721 gagcaattca tcgactgttt gctgcagcgc cagctgcgcc tgattttggc catgatcttc
    24781 gatgccgagc cgcattgaaa agagctccgg ctggatccag agaccgctct catggagatc
    24841 tactccgtgc cgctcaatga tcaccatggc cgcgcgtctg aaagctagca cctttgaagc
    24901 gggaagcgcc acatggatcc agtcacgatg aaacccgtct cgcccgcgcg cgcgctgctg
    24961 cgagcgattg gccaaaaagc ggcgagctac cgcatgccgg ttgcgccaaa acgtttccgc
    25021 ctcagtgatt ggtaattgct tggcgccgct tcgcaacaag acatcg
//
LOCUS       DASUVS010000055         4051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1767018, whole genome shotgun sequence.
ACCESSION   DASUVS010000055 DASUVS010000000
VERSION     DASUVS010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767018"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1279)
                     /locus_tag="VF182_03170"
     CDS             complement(200..1279)
                     /locus_tag="VF182_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436105.1"
                     /translation="MTPKKTARLVLFTAIISAVWVNLTFSADTPFYRGKTLTVLINFA
                     SGGPTDIEGRLVARFLGKHVPGQPTLVVQNMPGAGGVTGTNYLGEIAKPDGLILGYFT
                     SQFFNLLTADPNLRVDLTKFAYIASIEGVAVAYVRKDVPPGIQEAKDILKAKGLKAGG
                     LSLNSAKDVRFRLQLDILGVPHQYVTGYNSNSDARLAVERNEIQFFTEGTPGYRSQVQ
                     PRMVKEGVVMPLYADELVTVDGEFRPSAEVPDIPSFSQLHQQIFGKLGSGALWETLKT
                     INLTHAMQRYAKVAPNTPPEAVAALRQGFSQMIKDPEFIQEFKRVTRSEPAFQVGGEA
                     DRVIRKLVQAPPEVKDTIRKYTESK"
     gene            1825..2210
                     /locus_tag="VF182_03175"
                     /pseudo
     CDS             1825..2210
                     /locus_tag="VF182_03175"
                     /inference="COORDINATES: protein motif:HMM:NF021516.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2243 domain-containing protein"
     gene            2319..2915
                     /locus_tag="VF182_03180"
     CDS             2319..2915
                     /locus_tag="VF182_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX6436106.1"
                     /translation="MTLSFWFDFASTYSYPAAMRIERLAKERGVPLSWNAFLLSRIFQ
                     AQGWTDSPFNLYPIKGRYMWRDLERICEAQNLPFRRPSQFPRNGLLAARIACRFSRES
                     WTPAFVRAVYYANFAEDQDIADRAVIAACLRFVNQDTETVLHAADSAETKTMLREQTE
                     RAVDLGIFGAPSFIAGEELFWGNDRLEEALSWCQGPKK"
     gene            3243..>4051
                     /locus_tag="VF182_03185"
     CDS             3243..>4051
                     /locus_tag="VF182_03185"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436107.1"
                     /translation="MITKVLWLLATFLLAYVQLDAGEHPAKSPRLGFLFFGSKDQPHL
                     ESFHYGLRDLGYVDGKNIRIEYRYAERSSERLAVLAAELVALNPDVILTTTPAATRAV
                     LQASSKIPIVAIGFDPIAAGLVKSLARPGGNVTGLSSSAGPGMMGKRLDLLKEAFPKI
                     DVVAMLWNPEAGHFAKQVLDGAETGAKSLGLQLQPHEIRNTGDIDRAFEELKSLNPDA
                     LLIPGGAVMTLNSKRLVNLATKLRLPAMYQTRQFVEEGGLMAYGVNYGDLYR"
BASE COUNT          895 a         1093 c         1121 g          942 t
ORIGIN      
        1 gatcgaagcg gccgccaagg agtacagctg atagcgccag cgcgatctat atggaatgag
       61 ccgccttccc accgccgctc agctgtctgc gcagcggcac ccatggataa ggtaggaaat
      121 tgcctacctc gcagatgatg cggatcaggc gattgcttca gccggcgctg agcatgcaac
      181 cttacagaaa actctccaat cacttgctct cggtgtattt tcgaatggta tccttcacct
      241 cgggcggggc ttgaaccagc tttcttatca ccctgtccgc ctcgccgccg acttggaacg
      301 ccggctcaga gcgggtaacc cttttgaact cttggatgaa ttccggatcc ttgatcatct
      361 gcgaaaagcc ctggcgcagc gccgcgaccg cttccggcgg tgtattcgga gcaaccttgg
      421 cgtagcgctg catggcgtgg gtaagattga tagttttcag agtctcccac aacgcgcccg
      481 aaccgagctt gccgaatatc tgctgatgga gctgcgagaa agaaggtata tcgggcacct
      541 cagcgctagg acggaattcc ccatcgacgg ttacgagctc gtctgcatac agcggcatga
      601 cgacgccctc cttcaccatg cgcggttgga cctgcgatcg atacccgggt gtcccttcag
      661 taaagaattg aatctcgtta cgctccacgg ccaggcgcgc atcggagttt gagttgtatc
      721 cggtaacgta ttgatgggga acacctagga tatcgagctg caaccggaaa cgaacatcct
      781 tggcgctgtt gaggctcaag ccgcccgcct tcaaaccctt ggctttaagt atatctttcg
      841 cctcctgaat gccgggcgga acatccttgc gtacatacgc caccgcgact ccctcgatgc
      901 tcgcgatgta ggcgaatttg gtcaggtcga cgcgcaggtt gggatcggca gtcaacagat
      961 taaagaactg tgacgtgaaa tatccgagaa tcaatccgtc gggtttggca atctcgccga
     1021 ggtaattggt gccggtgacg ccgcccgccc cgggcatgtt ctgaacgacg agcgtcggct
     1081 gaccagggac gtgttttccc aggaagcgcg cgaccaatcg tccttcaatg tcagtgggtc
     1141 cgcccgaggc gaaattgatt aagacggtca atgtcttgcc gcgatagaaa ggtgtgtcgg
     1201 ctgaaaaagt aagattcacc cagacggcgc taatgatcgc cgtgaaaagc acaagcctag
     1261 ctgtcttctt cggtgtcatc gtgtctctct ttcgtggtta atgagctctt acattatcag
     1321 gattgttact gcggggtcca cgttgaatgt atcagcctca actcgcgatc ggtcttcatg
     1381 gatagaggcc ttcatcgata gatacaagcg ataaaattga gattgcgcca aaatatgact
     1441 ttttcttcgg accggcaaga tatttaagtt gcctggttgc tattgattca gcagcgagcc
     1501 cgatcgaaag atcccgcttt aaccgcttgc ggcagaatct cgcaactgca acactcgcgt
     1561 ggacagcgga tgctttcgat tcgaaaatcc cttcagatcg gtcgataaga ggacgagtgt
     1621 ttgtcgagga ttaggctgta gatccatgcg aaaaacgcag cgcgcacggt ggcaacggcg
     1681 atccgtcgat tgcgcgactt cccaaggagg agcaatggag taccgatcgg cggctcccac
     1741 gcaaacaaca cgctcaatta gcccgggcat cgtcttgggg ctgggattgg gtggttcata
     1801 gatgggattg cgctgcacca gattgtgcaa tggcataaca tgggatccgc caaggttccg
     1861 ccgatcacga tggaggcgat gcaacaaaac atggcctggg acggctggtt tcacgttgcc
     1921 accttggtgc tcaccatctt cggtgtttat ttgctcttga atcatgcgcg agcgaggcgc
     1981 tattgccgcg tgcgggatca ttcaccggcc aactgttgct cggctggggc gcattcaacc
     2041 tggtcgagga catcgaccat catcttcttg agatccatca cgtccgcgac ctgcccacgc
     2101 atgtgccagt ctatgactgg ctgtttctcg gcattggcgg tgtcggctgc tggtcatcgg
     2161 ctggtggctc gcgcggtcgg aggtggagct tcaccggcca taaggcttaa caggttgctt
     2221 cttgatcaag cgagcggaga attcctgcta tcgagaaacg cgatgatagc ttcgaagata
     2281 ttctcgacat cgtaaacgac taggtatcgg gtagacgaat gacactttcc ttttggttcg
     2341 atttcgccag cacctactca tatcccgcgg cgatgcgaat cgagcgactc gcaaaggagc
     2401 gcggcgtgcc gctatcatgg aatgcctttt tgctcagccg gatttttcag gcgcagggct
     2461 ggaccgactc gccttttaac ttatacccga taaagggccg ttacatgtgg cgtgatttgg
     2521 agcgcatctg cgaagcgcaa aacctgccat tccgccgtcc atcacagttc ccgcgcaacg
     2581 ggctactcgc cgcgagaatc gcttgccgtt tctcaagaga atcctggact ccggcattcg
     2641 ttcgcgcggt ctattacgcg aacttcgccg aagatcaaga catcgccgac cgcgctgtga
     2701 tcgccgcctg tttgagattc gtgaatcagg atacagaaac ggtcctccac gccgcggatt
     2761 cggctgagac aaagaccatg ctaagagagc agacggaacg agccgtggat ctaggcatct
     2821 tcggggcgcc tagcttcatt gccggcgagg aactgttctg gggaaatgat cggttggagg
     2881 aagctttgag ctggtgccaa ggtcctaaga agtaggagat ctgcccgggc ggctcattat
     2941 tacggcgacg gcgcataact aaatacaaag attttattcc cgtcatcaga aaacattcca
     3001 atggttggtc ttaattgttg agctaggttt tgctgaaaag cgagactatc tcgcgaacgc
     3061 gttccattaa ccttacactc gatccgagcg caggcagtct cggtgatgca gctattatag
     3121 cgcagcaaat ctgatactcc tgtgtagtca aacggccacc cgtcttcgct ttcgcgcctt
     3181 ctaaattatt attttcatgg agttcgtttt gtggcatatc cgttgcggta ggagcaatgg
     3241 ctatgatcac aaaggtcctc tggctgctcg ccactttcct tctcgcgtac gttcagctcg
     3301 atgcggggga gcatccggcg aagagtcctc gcctaggatt tctctttttc ggctcgaagg
     3361 accaacctca tctcgaatca tttcactacg gactgcggga tttaggttac gttgacggca
     3421 agaacatccg gatcgaatac cggtacgctg aacggagttc cgagaggctg gcagtgcttg
     3481 cggccgaact cgttgccctc aatcctgacg ttatacttac aactacgcct gccgccactc
     3541 gcgctgttct tcaggcaagt agcaagattc ctatcgtagc catcgggttt gatccgatcg
     3601 ccgccggctt ggtcaaaagc ctggcacgcc cgggcggaaa cgttaccggc ctttcaagca
     3661 gcgctggccc tggaatgatg ggcaagcgcc tggatctgct caaggaggcg ttcccgaaaa
     3721 ttgacgttgt cgcgatgctg tggaatcccg aagctggtca ctttgcaaaa caagttctcg
     3781 acggagcgga aacgggagcc aagtctctcg ggctacaact ccagcctcat gaaatcagaa
     3841 acactggaga catcgaccgg gctttcgaag agttgaaaag tctcaacccc gacgcgctgc
     3901 ttataccggg cggcgcggtc atgaccttaa attcgaaacg gcttgtgaat ctcgccacga
     3961 agcttcgctt gccggcaatg tatcaaacga ggcagtttgt cgaagagggc gggctgatgg
     4021 cctatggagt taattacgga gacctctatc g
//
LOCUS       DASUVS010000056        14228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1767309, whole genome shotgun sequence.
ACCESSION   DASUVS010000056 DASUVS010000000
VERSION     DASUVS010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14228
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767309"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1086
                     /locus_tag="VF182_03190"
     CDS             91..1086
                     /locus_tag="VF182_03190"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436108.1"
                     /translation="MKSALIISALAVLLLVPRQAPGQERLKLIYSQFTMTNSATWFAR
                     EAGLFERHGLNADLIYVDSAPAVQALTAGAAPLATMSGGLAVGPYLNGLDMVMLAGWC
                     NLNGYQLITRPEIRRPEDLRGKVIGIGRYGAAADWGLRLILRKLGIDEHKDVQIVQAG
                     GGGPATRLGAMEAKKLDATVLDSPQTVQARRLGFRTLADGVELGIPFLQGGLVTRRSY
                     VKSNEDTVRRTVRVIVEAIHYAKTNREAALKIMQKYLRVQDREALEDAYESFVIKQFP
                     RAPYATPAAVEIIFQLAADRDPRAKTADPKGFVDMRFVREIEQSGAIDKLYAQTR"
     gene            1349..1489
                     /locus_tag="VF182_03195"
     CDS             1349..1489
                     /locus_tag="VF182_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436109.1"
                     /translation="MAKKATTKKKPAAKKSNAKKSTAKKSSAKKKTPTPKQKEPIPRY
                     PY"
     gene            complement(1621..2034)
                     /locus_tag="VF182_03200"
     CDS             complement(1621..2034)
                     /locus_tag="VF182_03200"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX6436110.1"
                     /translation="MAALTLEMAEKAVKAAQAKAREQGTPMTVTVVDEAGRLVLSARG
                     DGAGFFTPETSRAKAVAAAAFRRSTKEMVRLRDSSPAFWSNVSNVLHGEALPSIGAVP
                     VFSGGKVVGAVGCGGGSGEQDHLCAEAGAAAIANG"
     gene            2470..3414
                     /locus_tag="VF182_03205"
     CDS             2470..3414
                     /locus_tag="VF182_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6436111.1"
                     /translation="MKRVPLYSSSRPSIIGQAPPTKQLEQSQSPDQSRQTPAAQFAKT
                     SGRPRWRNFYIRNERRFLWLVAGGLISVLLVFTYSALKPPREITQEDIRAAVLHTLET
                     NTLPSPVAKAYDVVRSSVVRVNGWGAEGPVDDDNDKSVGSGVVIVDKGTILTNLHVVV
                     GAKRVQVVFADGLKSDATVVAIQPEHDLAVLQAETIPDDLVAATMRSTADLTLGDQVV
                     AVGFPFGIGPSVTAGVVSGLRREFRSPEGKRLLTNLIQFDAAVNPGSSGGPLVTAGGE
                     VVGIVTAILNPTDQRFFVGIGFAVPIENAAAAAGLPPF"
     gene            3420..4421
                     /locus_tag="VF182_03210"
     CDS             3420..4421
                     /locus_tag="VF182_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX6436112.1"
                     /translation="MENKEINRSDIASLMERILYEVKKIIVGQDHFLERVLVAVLAQG
                     HLLVEGVPGLAKTLTVKTLARTMRGSFRRIQFTPDLVPADLVGTRIYNQKSGDFATSL
                     GPVFTNLLLADEINRAPAKVQSALLEVMQERQVTIAGESHHVPEPFLVMATQNPIETE
                     GTYPLPEAQVDRFMMKVLVGYPTEEEEFVIVARVTGTANDVVPVATTDQLNSLRRECQ
                     GVYVDPSLMQYAVRLASATRDPERFGLSDLGKFVSFGASPRASIHLIEGARALAFLRG
                     RGYVLPEDVMDLVSDVFRHRLVLTYEALAEGMTADQLIHRIMQQIPAPEKPLETHVR"
     gene            4575..5588
                     /locus_tag="VF182_03215"
     CDS             4575..5588
                     /locus_tag="VF182_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX6436113.1"
                     /translation="MSVFHKAPNVIPGAVNEDFRPPAARNSGAEDILRRLEWTVIRRL
                     DGLLHGDYQTLFRGFGLDLADLREYQYHDDVRYIDWNVTARLQTPYVRVYNEDREVTA
                     WFLLDLSPSVDFGSNIKKQSISTEFVTMLARLLTRHGNRVGALFYGDSVDTVIPARTG
                     RRHVLDILHRVLSRPKRSDSHGTNLRDFFQIASRIMNRRSLVFVISDFISTPGWAKPL
                     AHLAQRHEIIAVRLYDPLEMELPDLGLLVMQDAETGEQLFVDTHDRGFRRRFAAAAER
                     RETELRSAFSQAGVDTLELSTHDDLVDAILRFADLRKRRSQLAVGGSLPKHLRRSHDF
                     SMA"
     gene            5569..6612
                     /locus_tag="VF182_03220"
     CDS             5569..6612
                     /locus_tag="VF182_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6436114.1"
                     /translation="MTFLWPEMLLLLLLVPVLVGAYFFVLNRKKQAALRYASLSIVKD
                     AMSARQRFRRHIPPLLFLISITAMIFAIARPAALVTLPSQHRTIILAMDVSGSMRAAD
                     VQPSRIAAAQAAAKAFVADQPSNVRIGVVSFAGTASIVQMPTQNREDIIAAIDRFQLQ
                     RATAIGSGIIVSLAAIFPDAGIDVSSFIYGRDGSRGVPLDQSRKTDKPAPKPVPPGSY
                     TSAAIILLTDGQRTTGPSSIEAAQMAADRGVRVFTVGIGTTSGETLGFEGWSMRVRLD
                     EASLKAIANLTHGEYFYAGTANDLKKVYQSLNSRLVLEKKETEITALFSAAAAVTALI
                     SGLLSLLWFNRIL"
     gene            7257..8213
                     /locus_tag="VF182_03225"
     CDS             7257..8213
                     /locus_tag="VF182_03225"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436115.1"
                     /translation="MIGKILWLLAALLLLHVQLAEAQQPKKVPRIGWLAFGRSRADRR
                     QPFIEGLRSLGWVEGENIIIERRYANENYTQLPELAAELVRLKVDVIVVRDSVAIRPA
                     AQSTKTIPIVAVVSGDPVADGLIDSLARPGGNVTGLTNISPQLAGKRLELLKEVVPRA
                     SNVTVLGPGANSDQLRSAAQSLGVRLQELHVQKSDQFVNVFEVAMKGGSNAVIVQPSA
                     LANYHRNEIVRLAAKHRLPAVYGVNWYVTSGGLMSYGPSITGLNHRAAYYVDKILKGA
                     KPAHLPVEQPIRFELFINLKAAKQIDLTIPPNVLARADNVIK"
     gene            8210..8938
                     /locus_tag="VF182_03230"
     CDS             8210..8938
                     /locus_tag="VF182_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436116.1"
                     /translation="MNLPFPSLTNRRSLLLLDVGIACVLLSQCATVGETASSCDRIRI
                     TSNALSLAEAEQYCHYATGERQKVETFWGGTWSEIIHIDVDDRYKISRALTTKTRGFM
                     EMPLTVVRAQRSALLHEIVHVYAPNTNRFLAEGLAAYLQQQLGGNPAFPNFGRDLREL
                     ARHRLSALTSLEALNNVRTPRPLRSVMQEQTAYILAGSFVQFLIGNYGLTMFRNLYEV
                     GSYDLVYSKDLAALEREWRIDLQR"
     gene            9007..9981
                     /locus_tag="VF182_03235"
     CDS             9007..9981
                     /locus_tag="VF182_03235"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436117.1"
                     /translation="MLRACSIITLVFAALTVHLPNVHPATKIPRIGVLLLSDGRGAPM
                     EGFRQGLNELGYVEGQNIFIERRFAKGNRERLSQLAAELVDNRVNLILTAGTAQAQAI
                     RQASTTIPIVLAISGDPVAIGLAASLARPGGNVTGLSMISPELSGKRLELLKEAAPNS
                     SRIGVLWDGMVPENRFDFHTMQVAAASIGLKLESLQIGAADDLEAAFSIAARNRLNAL
                     VVVGGGVLNRQKKTILAFEMRNRLPTMHEQLDFAESGGLMAYGVNIAEMFRRSAAYVD
                     KILRGAKAGELPVEQPKKFELLINLKTAKQIGVMIPPTVLARADKVIK"
     gene            complement(10264..10584)
                     /locus_tag="VF182_03240"
     CDS             complement(10264..10584)
                     /locus_tag="VF182_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX6436118.1"
                     /translation="MKQTCFVCLNVDCQSRGSEELMKELTSQVAEKSIDAEVKSYICF
                     GGCDIGPNIVVHPQKVWYAGVKKEDVPEIVAALASGGTVSRLDSADPALKEIVFSLLD
                     AGVF"
     gene            complement(10769..11245)
                     /locus_tag="VF182_03245"
     CDS             complement(10769..11245)
                     /locus_tag="VF182_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6436119.1"
                     /translation="MSKIRGLSHVVLYVNDLDKMVAFYRDVLGLVEYRENPGRMVFLT
                     PDPDIDDHQIALAKGREGDAKLIGHIAWSVDSPADVKAFYEKFKAAGVPIDHCVSHAY
                     QEMGNTVSCYFLDPEGNCLEIYALVAERDQARINRPLDLDKSVDEIVTQASGLSAA"
     gene            complement(11722..11991)
                     /locus_tag="VF182_03250"
     CDS             complement(11722..11991)
                     /locus_tag="VF182_03250"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX6436120.1"
                     /translation="MWNESGNGHIFVSHLPVAPAGKIYAVWIIAPASAPRYVGAVKTD
                     VTGQGGLHIVAARTDHPVETFAVTLEPEGVTAAPTGPMVLVSKQS"
     gene            12652..13518
                     /locus_tag="VF182_03255"
     CDS             12652..13518
                     /locus_tag="VF182_03255"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX6436121.1"
                     /translation="MESIAASTLTTANEKSRRLRELVRAPEILIMPGAYDVLSALLFA
                     QMGFKAIQGTSGGIAAAVGFPDGEAMSRDQFIELSGNFAAAVSLPFNADGERGYGDEN
                     GVRDTVRALVSRGVAGMNLEDGAEKGKGGLVEVSEQLRKIKAVMDTKRELGSEFFLNA
                     RVDSFHVITDDPKKAVDEAIQRGNAYAEAGADCIFYLFLHSAEIIGRLAKEVKAPISI
                     LAGPQSPNVQQLQDLGVARVSYGSGFTKAAITATRRLAQEILEKGTCNLLKEGMQTPD
                     INALVGRKQKRA"
     gene            13515..14021
                     /locus_tag="VF182_03260"
     CDS             13515..14021
                     /locus_tag="VF182_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008894007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX6436122.1"
                     /translation="MNGRAAVVSLHIAFAGAAPVISLGEIQAIAGKGLEGDRYYLSTG
                     TYSKTRGTGREVTMIEIEAIEALGREYQIDIDAAQARRNIVTRGVALNHLIDREFTVG
                     EAVLRGTRLCEPCAHLEKLTAKGVMRGLVHRGGLRADVVRGGTIRVGDTITVTSEKIV
                     PVSPSANG"
BASE COUNT         3237 a         3910 c         3928 g         3153 t
ORIGIN      
        1 gacaggttat gctttgcgcg tccaaaggat tccccaaggt cgatctaacg tcttttctgc
       61 tcgattagca ctctctttaa ggagtctcct atgaaaagcg cgttgatcat tagcgctctt
      121 gccgtcttac ttttggtccc acggcaagca cccgggcaag agcggcttaa actgatctat
      181 agccaattta ccatgaccaa ctcggcgact tggttcgcgc gcgaggctgg gctgttcgag
      241 cgtcacggct tgaacgcgga cctgatctat gtggactccg ctccggccgt gcaggcgctc
      301 actgccggcg cagcgcctct ggcgaccatg tcaggcggcc ttgcggtcgg gccgtatctc
      361 aacggcttgg acatggtcat gctggcgggc tggtgcaatt tgaacgggta ccagcttatt
      421 actcgtcccg aaattcggcg ccctgaggat ctgcggggaa aagttatcgg catcgggcgc
      481 tacggcgctg ccgcggactg gggtttgcga ttaattcttc gcaagctcgg tatcgatgag
      541 cataaggatg tacagatcgt tcaggcaggc ggcggaggac cggcgactcg tcttggcgcc
      601 atggaagcca aaaagctcga tgccacggtg ctcgattcgc cgcaaaccgt gcaggcgcga
      661 cgcctgggat tccgcaccct ggctgacggc gtcgaactgg gtattccctt tctgcagggc
      721 gggctggtga cgcggcgctc ttacgtcaag tccaatgaag acacggttcg gcgaaccgtt
      781 cgagttattg tcgaagcaat ccattatgct aaaaccaatc gcgaagcagc gctgaagatc
      841 atgcaaaaat atctgcgcgt gcaagatcga gaagccctgg aagacgccta tgaatcgttc
      901 gtcatcaaac agtttccgcg ggcgccgtac gccacgcccg ccgccgtcga gattatcttc
      961 caactcgccg ccgatcgcga cccgcgcgcc aagaccgccg atcccaaggg gttcgtcgac
     1021 atgcgtttcg tccgggaaat tgagcagagc ggcgcaatcg ataaattata tgcccagacg
     1081 cgatagcctc ggcctagctt atagagtgct cggcttcgga aacggtgcga tgcgattttt
     1141 caagtgcggg gttttgccgt tgggccaaca tgccccggga ttactcattg aaagtttttt
     1201 tccgagacgg acaaggttcg caaaatggcc gaacgttaat tttttctatt ctttctgcga
     1261 tgattaaagt attatcaggc acgcataaaa cgtcagttgc ggagctgagc tcacggtaaa
     1321 aacttccgag ataaaggaga acatttgaat ggcgaaaaag gcaaccacta agaaaaagcc
     1381 ggcggcgaaa aaaagtaacg ccaaaaaatc cactgctaag aaatcgagcg ccaagaaaaa
     1441 gacccccaca ccgaagcaga aagagccgat tccgcgctat ccttattaac ggaaaccgtt
     1501 cttcgacacg gattccggac taccgcgacc atccctcatc gcgagtcgag cggcaggaga
     1561 cagaacgggt cctctgctgc tcgattggca aatctgtggt agccggttgc cgaacgcgag
     1621 ttacccgtta gctatagctg cagcgccggc ctcggcacaa aggtgatctt gctcgccgct
     1681 accgccaccg cagccgacag cgccaaccac ttttccgcct gaaaaaactg gcactgcgcc
     1741 tatgctcggc agagcttcgc cgtgcagcac attagagaca ttgctccaga aggcgggcga
     1801 cgaatctctg agacgcacca tttctttggt tgatcgtcga aaggccgctg ccgcaaccgc
     1861 tttggcgcgg gaggtttcag gagtaaaaaa tccggcgcca tcaccgcgcg ccgacagcac
     1921 caagcggccg gcttcatcga caacggtgac cgtcatcggc gttccttgct cgcgcgcttt
     1981 cgcctgcgcc gccttgaccg ccttttctgc catctcaagc gttaatgcag ccatatctat
     2041 cgctcctttc taatttgcgc tccgtgcgtt tgcgattcac gtccctttaa aggagccgtg
     2101 aaacgcgaaa gaaatgcgaa cactccgagg aatcttccga cgatgtccaa gagccttctg
     2161 aatctgctaa aaccctgtca tcccggattt tctttgacct aagcgatata gaatcggagc
     2221 atcgccgctc gtcctttcga attttccgtg gcagcacgcg agtggctcca actactcttc
     2281 tcgaaggcga aagtattcga taggttgagg gtatgtcaaa ttaaacggac ttgcggcagg
     2341 gtcaataaag ggctttcata tcggttggcc ttgggataga tagagatcag cagaccctgg
     2401 ttgcttgcca cgagaagaac ttgacggagc tctattatag atgcttctcg aacctgatgg
     2461 atcgcacaca tgaaaagagt accgctctac agctctagcc ggccgtcgat cattggacag
     2521 gcgcctccga ctaaacaact cgaacaatcg caatccccgg atcaatcgcg ccaaacaccc
     2581 gccgctcaat tcgccaagac gtcaggccgg cctcgatggc gaaacttcta cattcgcaat
     2641 gagcggcggt ttttatggct tgttgccggc gggttgatat cggttcttct ggtatttact
     2701 tactccgccc tcaagccgcc gcgcgagatt acccaggaag atattcgagc tgcggtgctt
     2761 cataccttgg agaccaacac gctgccgtcg ccggtggcga aagcctacga tgtggttcgc
     2821 tcctcggttg tcagagtgaa cgggtgggga gccgagggtc cggtcgatga cgacaatgac
     2881 aagagcgtcg gtagcggagt cgtgatcgtc gacaagggga cgatcctcac aaatcttcac
     2941 gtggtcgtag gcgccaaacg cgtgcaagtg gtgttcgccg acggtctcaa atccgatgca
     3001 acggttgtag ccatacaacc ggagcacgat cttgcggtgc ttcaggcaga gacaataccc
     3061 gatgatctgg tcgcggcgac gatgcgatcg actgccgacc tcacgttagg cgatcaggta
     3121 gtcgccgtag gctttccatt cggcatcggt ccttccgtta ccgcgggcgt ggtgtcaggg
     3181 ttgcgccgcg aatttcgctc tccggaaggc aagcgcttgc taacaaattt gattcagttc
     3241 gatgccgcgg taaatcccgg aagctcgggc gggccacttg tgaccgccgg cggcgaagtc
     3301 gtcggcatcg tcaccgccat tctaaatcct acagaccagc gtttctttgt cgggatcggc
     3361 tttgctgttc ccatcgagaa cgcagcagcg gcagcgggat tgccgccatt ctaaggacaa
     3421 tggaaaacaa agagatcaat cgcagtgaca ttgcttcatt gatggagcga atcctttatg
     3481 aggtaaaaaa gattatcgtc gggcaggatc attttttaga gcgcgtgctg gttgcggtgc
     3541 tcgctcaggg gcacctgctc gtcgaaggcg tcccggggct ggccaagact ctgactgtaa
     3601 agaccctggc cagaaccatg cgcggttcgt tcaggagaat tcagtttacg cccgatcttg
     3661 tgccggccga tctggtggga acccggatct acaatcagaa gagcggcgac ttcgccactt
     3721 cgttgggccc cgttttcacc aatcttttgc tcgccgacga aatcaatcgg gcgccggcga
     3781 aagtgcaaag cgccctgctc gaagtgatgc aggaacgcca ggtcacgatc gccggcgaga
     3841 gccatcatgt ccccgagccg tttctggtga tggcgacgca aaatcccatc gagaccgagg
     3901 gaacctatcc gctgccggaa gcgcaggtcg accggtttat gatgaaagta ctggtgggct
     3961 atcccaccga ggaagaggaa tttgttatcg tcgcaagagt caccggcacg gccaacgatg
     4021 ttgtcccggt ggccacgacc gatcaactga actcgctccg gcgcgagtgc cagggcgtct
     4081 atgtcgatcc gtcactcatg caatacgcgg tgcgcctggc ctcggcgacg cgcgatccgg
     4141 aacgctttgg cctgtctgat ctaggtaagt tcgtctcgtt cggcgcaagc ccccgagcct
     4201 ccatccacct catcgaaggc gcgcgggcgc tggcattctt gcgcgggcgc ggctatgtct
     4261 tgcccgagga tgtaatggat ctcgtgtccg atgtttttcg tcaccgtctg gtgctgactt
     4321 atgaggcgct cgcggaggga atgaccgcgg atcagttgat ccaccggatc atgcagcaga
     4381 taccggcccc ggaaaagcca ctggagaccc atgtcaggtg acatacccgg ggcgaaatgc
     4441 ctggaaggtg atcgtgaatt ctaagcgaag cctagatatg cgcgcgagtc gccggagcgt
     4501 aaatacaaat caacgcggaa gcgctggaag gcgagctccg tttccagctt ccatcatcca
     4561 tcttcatccg atccatgagc gtttttcaca aagcgcctaa tgtaattccc ggcgctgtca
     4621 acgaagactt ccgtcccccc gcagctcgca actccggagc tgaagacatt ctacggcgct
     4681 tggagtggac ggtgattcgt cgtctcgatg gattgctgca cggcgattat caaactttgt
     4741 ttcgcggttt tgggctggac ttggctgatc tgcgcgaata ccaatatcac gacgacgtcc
     4801 gttatatcga ctggaacgtc acagcgcggc tccagacgcc ttatgtccgc gtctacaatg
     4861 aagatcgaga agtgaccgca tggttcctgc tcgacctgag cccgtcagtg gattttggtt
     4921 ccaacattaa gaagcagtcg atctcgacag aattcgtgac aatgctggcg cggctcttaa
     4981 ctcgacacgg caatcgcgtc ggcgcgctat tctacggcga cagcgtggat acggtgattc
     5041 cggcgcggac cggccgccgt cacgtactcg atattctcca tcgcgtgctt tcccggccca
     5101 agcgttccga ctctcatggg accaacctgc gcgatttttt ccagatcgcc tctcgcatca
     5161 tgaatcgacg ctcgctggtc ttcgtgattt ccgatttcat cagcacaccc ggctgggcca
     5221 agccgctggc gcatttggcg cagcggcacg aaatcattgc ggtgcgactt tacgatccac
     5281 tggaaatgga attgccggat ctcgggctgc tggtgatgca ggatgccgaa acgggagaac
     5341 agttattcgt cgatacgcac gaccgcggtt tcagaaggcg cttcgcagcc gcggccgagc
     5401 gccgcgagac cgagcttcgc tccgctttta gccaggccgg cgtcgacact ttggagttgt
     5461 ccacccacga tgatctcgtc gatgcgattc tccgtttcgc cgatctgcgc aagcgccgca
     5521 gccagttggc agtgggcgga agcctgccaa aacatttgag gaggagccat gacttttcta
     5581 tggcctgaaa tgcttttgct tctcctgctg gtgccggttc tggtgggcgc atacttcttt
     5641 gtcttgaatc gcaagaagca agctgccctc agatatgcca gcttgagcat cgtcaaagac
     5701 gcgatgagtg ccaggcagag attccgccgt cacattccac ccttgctgtt tctgatttcc
     5761 atcactgcga tgatatttgc gatcgcccgg ccggcggcgc tggtcaccct gccgtcgcaa
     5821 caccggacta ttattctcgc catggatgtg tcgggcagca tgcgcgctgc cgacgtgcag
     5881 cccagccgga ttgccgcggc acaggcggcg gcgaaagcct tcgttgccga tcaaccctcc
     5941 aacgtgcgca tcggcgtggt gtcctttgct ggaacagcat cgatcgtgca gatgccgacg
     6001 caaaatcgcg aagatattat tgccgccatc gatcgcttcc aacttcaacg cgctaccgcc
     6061 atcgggagcg gcatcattgt ctcgttggcg gcgatctttc ctgatgccgg catcgatgtg
     6121 agctcattca tttacgggcg ggacggttcc cgcggtgtgc cgctcgatca gtctcgcaaa
     6181 acggacaagc cggcgccgaa accggttccg cccggatctt acacttccgc ggcgatcatc
     6241 ttgctcacgg acgggcagcg gacgacgggg ccgagctcta tcgaggcggc tcaaatggcc
     6301 gccgatcgcg gcgttcgtgt ctttacggtg ggaataggaa ctacaagcgg cgagacgctc
     6361 ggcttcgaag gctggtccat gcgggttcgc ctggacgagg cctctctcaa agcgatcgcc
     6421 aacctaacgc acggcgagta tttctatgcg gggacggcaa acgatttgaa gaaagtttac
     6481 cagtcgctca attcacggct ggtgttggag aagaaagaaa ccgagatcac tgctctgttt
     6541 tccgcagccg cagcggtcac tgcgctaatt tccggtttgc tttcgttgct gtggttcaac
     6601 cgaatcctgt agcctcgcac aggcaggcac cgctgaaggg tgcgattgaa tgtcccaaat
     6661 tcgcgccgtg agcaagaaac ccgctcaagg tggtgacagg aatcctcgtg acaccaggtt
     6721 agttgaggca tcgacgaggc gggataggct agccgtcaga cctcccgaca tgaatccgta
     6781 ccactaatat caagttctcg acggacgcga ttacctacga gctgaagtca gccgacgcac
     6841 tgcgcaacgc tcgatgacta gtgctgtcgt cagcgatggc agcaagggac tgcggcagag
     6901 tgggcttgga gttccattgc ggtcgtcaga ttccgcttct acacattggc aatatctgcg
     6961 ttctgccgac agatgacatt gagcgcgcgc actcgatagt ccgtactcgt ttggcgcaag
     7021 ctggagcgac ggctcgcctg gctgcttatt gagacatcga gacgaacgca gcggtaccgc
     7081 ttggcgacgg tagtcggcgt tctggcagca gattgctcgt ccgagcaatt gacgaatcaa
     7141 aactgatact ccgcctgtag tggaagcact acgctctcct aattgtgctc caaagaattc
     7201 ggtgttcata agcttccttt tatggcatat ccgttgctgc gctagaaaga aacgccatga
     7261 tcggaaagat cctctggcta ctcgcggctc tcctcctcct tcacgttcag cttgccgagg
     7321 cgcagcagcc taagaaagta cctcggatcg gttggctcgc ctttggacgt tccagggctg
     7381 atcggcggca gccattcatc gaaggtctac gctccttggg ctgggtcgaa ggagaaaaca
     7441 ttatcatcga acgccgctat gccaacgaaa actatacgca gcttccggag ctagcggccg
     7501 agctagtccg tctcaaagtt gatgttatcg ttgtacggga ctcagtagcg atccggcctg
     7561 cggcgcagtc aactaaaacg attccaatcg tcgccgtcgt cagtggcgat cccgttgccg
     7621 atggcttgat tgatagcctg gcacggccag gggggaacgt caccggtctt acgaatatct
     7681 caccgcagct cgccggcaag cggctagaac tgcttaaaga ggtagttccg cgagcttcga
     7741 acgtgaccgt acttgggccg ggtgccaatt ccgatcagct tcgatctgcg gcgcagtctt
     7801 tgggcgtacg gctccaagag ctccatgtcc aaaaatctga tcagttcgta aatgtctttg
     7861 aagttgctat gaagggaggt tccaacgcag ttattgtgca gccgtctgct cttgctaatt
     7921 accaccgaaa cgaaattgta cgccttgctg ctaagcaccg gttaccggcg gtgtatggcg
     7981 taaactggta tgtcacgagt ggcggcctca tgtcctatgg gccgagcatt accggattga
     8041 atcaccgcgc cgcttactat gtagacaaga tcctgaaggg agcaaagcca gctcatttgc
     8101 ccgtcgagca gccgataagg ttcgagcttt tcataaatct aaaagccgcc aagcagatcg
     8161 atttaacaat tcctcccaac gtgctggcac gagcggataa cgtgatcaaa tgaatttacc
     8221 ttttccatcg ttgacaaacc gccggagctt actcttatta gatgtcggta tcgcatgcgt
     8281 tcttcttagt cagtgtgcca cagtcggtga aactgcctca agttgcgaca ggattcggat
     8341 cacttccaat gctctgtcgc tcgcggaggc ggaacagtat tgtcattatg ccacgggcga
     8401 acgacaaaaa gtggaaacgt tctggggtgg aacatggagt gagataattc acattgatgt
     8461 ggatgatcgt tataaaattt cgagggcatt aaccacgaag acccggggtt tcatggagat
     8521 gccgcttact gtagtgcgag cgcaaagaag cgcgctgctg catgagattg tccatgtata
     8581 tgcgcctaac acgaatagat ttttggccga agggctagcc gcttatcttc aacaacagct
     8641 gggtggaaac cctgcgtttc cgaacttcgg tagggacttg cgggaattag cgcgacatag
     8701 gctttcagca ttaacctcac tggaagcgct aaacaatgta aggacgcccc gccccctgcg
     8761 ttctgtcatg caagagcaga ccgcatacat tcttgccggc tcatttgtcc aatttctcat
     8821 tggaaactat ggcctcacga tgttccgcaa cttatatgag gtgggtagct acgacttggt
     8881 atacagcaag gacctagctg ctttggaaag agaatggcgg attgacctcc agcgataaga
     8941 ttcagcgatc aagctaatca ccgcgacctg cattgcgcat gtaaaattgc gttaaatgga
     9001 ggactgatgc tcagggcgtg cagtatcatc acattagttt tcgccgcact aaccgtacat
     9061 ctgcccaacg tacatccggc cacgaagatt ccgcggatag gagttttgct tctgagtgat
     9121 ggccgaggcg ctccgatgga agggtttcgc caaggcttaa acgaactcgg ctatgttgaa
     9181 gggcagaata tttttattga gcgcagattc gcgaagggaa atcgtgaacg tctttcgcaa
     9241 ctggcagccg aactagtcga caatagggtc aatctgatac ttacggcagg cactgcccag
     9301 gcccaagcta ttcgacaggc aagcactaca attcctatcg ttttagccat cagtggcgac
     9361 ccagtcgcaa tcgggttggc ggcaagcctc gcccggccag gtggtaacgt tacggggctc
     9421 tcgatgatct cgccggaact aagcggaaaa cgattagaac tgctcaaaga ggccgcgccc
     9481 aacagctcgc gtatcggtgt tctatgggac ggcatggttc cagaaaacag gttcgatttt
     9541 catacaatgc aggtcgcggc tgcgagcata gggttgaaac ttgaatctct tcaaatagga
     9601 gcggcagatg atctcgaagc cgccttctcc attgcagctc gtaatcggtt aaacgctttg
     9661 gttgtagttg ggggtggtgt tctcaataga caaaaaaaga cgatattggc ctttgagatg
     9721 agaaatcgac taccgacaat gcacgagcag ttagattttg cagaatccgg tggtctcatg
     9781 gcctacggcg tgaatatagc tgaaatgttt cgccgttctg cagcgtacgt cgataagatt
     9841 ctcagaggcg ccaaagcagg tgagcttcct gtggaacagc ccaagaagtt cgagctgctg
     9901 ataaatctga aaaccgcgaa gcaaatcggt gtgatgattc caccaacggt gctcgcgcgc
     9961 gcggacaagg tgataaagta ggaaacgccc tggcaaagct cctaatcgaa aagagggtca
    10021 tcacggatgc ggagttcaca aaaaagctgc ttgaggtgct atctagaaca tggctctatc
    10081 gggagtatgc caccaggctt aagaacgttc ctgaaaacct tccaaaatgt gccaactccg
    10141 atgctaaaac gatagccctc ccccaacata cccaaaagaa aaaaagcccg gttggccgag
    10201 ctcttcttca atccgtttca cttcgtgttc ttaggcttag cgcctcgcat gccgggagct
    10261 tacctagaat actcctgcat ccagaagcga aaatacgatc tctttcaacg cggggtctgc
    10321 actgtcgagc ctgctgaccg tgccgccgct ggccaaggca gcaactatct ccggcacatc
    10381 ctcttttttg acgccagcgt accagacctt ttgcggatgg acaacgatgt tgggaccgat
    10441 atcacagccg ccaaaacaga tgtaggattt cacctcggca tcaatcgatt tttccgcgac
    10501 ctgactagtc agctccttca tgagctcttc agagccccgg ctctggcaat caacgttgag
    10561 gcagacaaag caagtctgtt tcattagaac tgaattcctt cctggtcttt agttaaccca
    10621 aaagacgcct cgatccaaga ccattgtgtt cgttcaaggg gcggcaagtt tgttcaaggt
    10681 tgaaccgtcc aaccgctcgc ggatgctcgc tggattgaga tggcctgcct gatgaaagcc
    10741 tttgaaattg caacagcaat gcggaatctt aggcggcact gagcccgctg gcctgcgtga
    10801 caatctcatc cacactcttg tctaaatcga gaggccgatt gatgcgcgct tggtcccgct
    10861 ccgccaccag agcatagatc tccagacagt tgccttccgg atcaaggaaa taacaggaga
    10921 cggtatttcc catttcctgg tatgcatggc tgacacagtg gtcgatgggc acacctgcgg
    10981 ccttgaactt ctcataaaat gccttgacgt cggcagggct atcgacgctc cacgcaatgt
    11041 gaccaatgag cttggcgtca ccctcgcgac cttttgccaa agcgatttga tgatcgtcaa
    11101 tgtcggggtc cggcgtgagg aaaaccatgc gaccggggtt ctcccgatac tcgaccagcc
    11161 cgagcacgtc gcggtagaaa gcgaccatct tgtccaggtc gttgacgtag agcacaacat
    11221 ggctcagccc tctgatcttt gacatcggca gcctcctttg tatagttccc atcgattgtt
    11281 cacgacggtt cctagagtga aaacctatcc gatagcttca tcgcatgtca acggtgtgga
    11341 cgctgagtgg cgcagtccat cagacctatt ccatcgtctc aacgtctaaa tcctctcagc
    11401 cgggggtgaa ccctgtgtta caagagacag gaggcccttt tatgccgatc tcacgccagt
    11461 agaaaatttg ctcaatattc tttgctcgac gctctcatcg cgcggcgctc gcggcgcttt
    11521 ggattggcat gtgcggatgg tcgaagcctg ttcagccatt gacaacttga gcgaacctct
    11581 tcggctaaac gtttggaacc attggaacag ctggaacgtc tgcatcattt cataagtgaa
    11641 gtggataact ttatgacttg gatgcaccat ggccagccat aaagaatgtt cgcggaaaag
    11701 atttcgagat gccgctacca tttaagattg cttcgacact aacaccatcg gtccggtggg
    11761 cgcggcggta acgccttcgg gttcgagcgt caccgcgaag gtctccaccg gatgatcggt
    11821 gcgagcggca acgatatgca agccgccttg gccggtgaca tcggtcttta ccgcgccgac
    11881 gtagcgcggc gccgaagctg gcgcgatgat ccagacggca tagatctttc ctgcaggcgc
    11941 aacgggaaga tgacttacaa agatatgacc gttgccggat tcattccaca agaatcgccc
    12001 ggcagcttaa gataagccgg cagaccggga tctccctctt gcgcgacaaa tcagtctttt
    12061 aatggtttat gcacggcgct tcatgacgcg gtttcatggc accggcacac tgacgactcg
    12121 ctggctaccg cttacctctg cagcttttat cgttctcttc ggcatagcgc tgacatggca
    12181 agctctgcgg agcgccggtt ttctggggat cgggatctag ccgacgattg aaccagttcg
    12241 attttagttc acgagtgata atttccgata aatccgtgcc ttcgtcagtg tctttccagc
    12301 ttcaatcttt ttcggcgaag cacaggacgc ggtgccccgg actcccccgt cggcaataaa
    12361 acaactctga aactgtgtga tacgacggta gctctccgaa ctcttgctat attgtgctgt
    12421 tccgccttag gatgcgagct caacaactaa aaatgttcaa gacagtcacc gttcaattcg
    12481 tttaagccgt tgaacgagtt tagaactgca aaaagggttc aaaaggtaag tctttcaaaa
    12541 agcgacgccc tagccatggg gcgaaacaaa aactcggatg atccgaacga gttgaaccgc
    12601 ttgaaccatt tgaacgaatt gaacttttaa aatctaagag gagaagggat tatggaatcg
    12661 attgcagctt caacattaac tacagccaac gagaagagcc gcaggctcag ggaactggtg
    12721 cgcgcgccgg aaattctgat catgcccggg gcctatgacg ttctcagcgc gctgctgttc
    12781 gcgcagatgg gcttcaaggc gattcagggg accagcggcg gcattgctgc agccgtcggt
    12841 tttccggatg gcgaagcgat gagccgcgat cagtttatag agctcagcgg caacttcgcg
    12901 gcggcggtga gcctcccgtt caacgccgac ggtgaacggg gttacggcga cgagaacggc
    12961 gtccgtgata cagtccgcgc tttagtgtcc cggggggtcg cgggtatgaa tctcgaagac
    13021 ggcgcggaga agggcaaagg cggattggtc gaagtatcgg aacaattacg caaaatcaaa
    13081 gccgtcatgg acaccaagcg ggagttgggc agcgagtttt ttctcaatgc gcgtgtcgat
    13141 tctttccacg tgatcaccga cgatccaaag aaagcggttg atgaagcgat ccagcgcggc
    13201 aatgcctacg ccgaagcagg agcggactgc attttttacc tattcctcca ctcggccgag
    13261 attattgggc ggctagccaa agaggtcaaa gcgcccatca gcattctcgc cggtccgcag
    13321 tcccccaacg ttcagcaatt acaagacctg ggcgtcgcgc gggtcagcta cgggtcgggt
    13381 ttcaccaagg ccgcgatcac cgccacgcgg cgattggccc aagagattct cgaaaagggc
    13441 acatgcaatc tgctgaagga agggatgcag acgcccgata tcaacgcgct ggtggggcgc
    13501 aaacagaagc gagcatgaac gggagagccg ccgttgtgtc cctccatatc gcttttgccg
    13561 gggccgcccc agtgatctca cttggggaga tccaggccat tgccggcaag gggctggaag
    13621 gagaccgcta ctatctcagc accggcacct acagcaaaac tcgcggaacc ggccgcgaag
    13681 taaccatgat cgaaatcgaa gccatcgagg ctttaggacg agagtatcag atcgacatcg
    13741 atgcagcgca agccagacgc aacatcgtga ctcgaggcgt cgcgctcaat catttgatcg
    13801 atcgagaatt taccgtcggt gaggcggtgc tacgcggcac caggctgtgc gagccatgcg
    13861 cccatttgga aaaactcacg gccaaagggg ttatgcgggg attggttcat cgcggcggct
    13921 tgcgcgcgga tgtggtacgc ggcggcacta tccgggtcgg agatacgatt accgtgacga
    13981 gcgaaaagat cgtcccagtt tcaccaagtg ctaacggttg aaaagccgca ggttctgcag
    14041 atcccgcgtc cgaaccccga gaatcagcaa gccacatagt acttagacgt cactcgtgca
    14101 ttcccgagta tgaacaacag aacagcgtcg catccgctgc agacgttcca gtaagatgct
    14161 caccctaaac cggcattcgt cgccgagctt atctctccga tattgccgtc gcaaacgcta
    14221 ttttgcgg
//
LOCUS       DASUVS010000057        15454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1112641, whole genome shotgun sequence.
ACCESSION   DASUVS010000057 DASUVS010000000
VERSION     DASUVS010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 15454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15454
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112641"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VF182_03265"
     CDS             complement(<1..771)
                     /locus_tag="VF182_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436123.1"
                     /translation="MRYNTKIRALGIALLLAMSSATISFGAGAELSKLRFAQSGHTAA
                     SWPIYIAQEKKIFEKNGIQFENIIIQGATNTTRAVLSETIPLGRINPDYVIGGIEKGA
                     KVRIIGGNMDKIPYDLMARTEIKNGAELKGKTIGVSTLTGGTTNMIEEVLLKGFKLKP
                     GEYRYLVVGTSPERYAALKSGSVQATFMGPPFNFRAQQDGFTKLITFHEILGPIQFTA
                     DFGHMNYLKSNRNEVVRYLKSMIEATNWLYDTKNKEEAL"
     gene            complement(797..1597)
                     /locus_tag="VF182_03270"
     CDS             complement(797..1597)
                     /locus_tag="VF182_03270"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6436124.1"
                     /translation="MAGPALVLRNLNKAITRPDRDPFVIFRNINLSVSDGEFVSIVGP
                     SGCGKTTLLRVINGLMPHSGGEILIDGRSAQEVDNELLMGFVFQGASLLPWRTSLKNV
                     VLGLEGRNHDSKEAERTAQKFLDLVGLSGFETHYPHELSGGMQQRVNLARALAVNPRI
                     LLMDEPFAALDAQTRNFMQLELLRIWRETKKTVIFVTHMIAEAILLSDRVIVFSHRPG
                     SIRSEVNVPLPRPRTMEMKSDARFLELEQILWKQIEEEVNASGGFSLM"
     gene            complement(1599..2381)
                     /locus_tag="VF182_03275"
     CDS             complement(1599..2381)
                     /locus_tag="VF182_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6436125.1"
                     /translation="MKVRNLKLTSTFLKAGSVIGGLLLWHVLATAVVKDPTLLVSPVV
                     VAVTAYQMLFVTKDLYPHIFASSWIFFYGFVLAILAGVPLGFLMALNTVARDYVNPWM
                     SMLYTTPRIAFAPVLLLWLGIGAGSKIAIVFLGCFFPILINSYYGMRVVNRDYVELAR
                     SFRLSRSALFLKILLPASVPFILAGIRLSVGRGLTGVAIAEWFGAREGLGYLIFFAGQ
                     TLNIPILFVGVAVFAALGIAGFEIIRIIEIYALPWRKEPQGS"
     gene            complement(2401..3171)
                     /locus_tag="VF182_03280"
     CDS             complement(2401..3171)
                     /locus_tag="VF182_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6436126.1"
                     /translation="MPKKEIFRGSLSIIIGLTIWEASTRLLLDNELLIPPPSSVLISF
                     WNLLSTGELNRHLVATLIEFICGFTTACFIGIILGYLMGMYKWFDEIMDPWIATLYSI
                     PIITVVPLIIIWFGIGIFSKVVVVFKITAVAIILNTAAGIKSLDPVWLELAKSLRLSD
                     WQTSYKIRLPGALPFIITGMRLGVGRALLGVIVAELMAANAGLGFLLRDSSETWDSPK
                     LFVTVILLAAMGLVSFNLIKKLEQRMAPWRQSAEWSSE"
     gene            complement(3185..5698)
                     /locus_tag="VF182_03285"
     CDS             complement(3185..5698)
                     /locus_tag="VF182_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436127.1"
                     /translation="MRKSWISALSLSLALGAIDGYPDPVRGQDSQAMIERILNPLPEF
                     DPFEKPPQAPKFFPDEVDKRARELLIDSLTNDKAAIAEHAEFFQTEDRRLQKQHGSST
                     GLSDHAQDLLNNTVDERQRYLAALRQALKNTSSPERKKYLQAIIDEDDLTRADQLTRQ
                     SSVNELGGVFNRLLSSVDLIGVASGNYIGAAAETAIGQAYSLLNTEMSVEERRALTRH
                     MDHLKRYPNDPRNGKIVKDIEELEKKKTAALIRKQLDHSKQAAQKGDIDKAFFYAEVA
                     SYLDGRSRIVQAELQKLSKSYLHRSEQQYQGLSAQAEKPGSTEQQEDVEELLLALSLR
                     DQQQIAQQAATLEKNYAGKPLADSARDASSVALEMQGRHEEAKKIIERLASSAKTPAA
                     QKHAAALLQSPEYNLLATFEAARSERRAESVKYVLLGEDLLKKNLIYAAGAVAAAGPA
                     GAVTLGAANAMMVGNNLINVLSNNPVSAQSVIDAGVSYVRSHPDSVSATEVYRILANT
                     YEEKGMIDKAIAYHELAGTSAEKLSSLKQKAATALLNAATKSKGRGTQEYYLTTLLDH
                     YPESPAAADATKKLAELAKTDNSGLRMSKEFLKENPELFGPTGLGLKASLFDGNTRNM
                     ELADRGVAITGDNQLLIYYQTPSGVRSQSYSLPRQTTERFFVALRQKNHAVALADAGQ
                     RGKGSIGGIKNLPMPIIQGARPNAGESEQEHEDTTFSFVREASSHAPAFPKVLDHEML
                     SENERDPAGKYSLPPIQGSVSASRFSMSGALPAGLWGNQIGIGGDHKGSFAGVQLPIP
                     LLQGFIPVDFMVQGRPGGVSVYPKIHMRDDNGEDQELYK"
     gene            complement(5695..6591)
                     /locus_tag="VF182_03290"
     CDS             complement(5695..6591)
                     /locus_tag="VF182_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436128.1"
                     /translation="MISVTTVLSLLILAGCSSRIASPEARYAAAGDLLDIVKDFQRLA
                     KEDTYRFAIPKDVTGVNIMKATLVRLDDYEKKNAGKFTDIIQFNKALALERLREYANA
                     TVLYRNVSEIGGRLGAEAAKNLEALESFLNILDKPLPTEDPFQYIKGLDEKVAAWNHL
                     IEKHQGTTYEFLARCEEERIDRAKVAFVEANRYRLKDGNQLVILGYGQLITKHRQSKN
                     IYRFIIDFGDFYALLAKEYAAQYDPEGLSFELKAFDQFAKSALKLYTEVVQVDGIVEK
                     IEAQGNIEAMRGLIEKVTRLSR"
     gene            6859..8031
                     /locus_tag="VF182_03295"
     CDS             6859..8031
                     /locus_tag="VF182_03295"
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEX6436129.1"
                     /translation="MKVFLQLLVLPILFASFIVGCVPAAHEESGSAPRDPGATAAASL
                     PEYQGPATRPFVMGFTRWPADLTPEGVAAAQKFADLHGDIMAVMFIGGIPWPEALEGK
                     PYPKDVQDALADRPPSEKKLFLSISPLDRDRKGLAPYWGEKENEPLPKPWDKEPLNSP
                     RVKKAFLNFVLHSVKAMRPDYLAIGVESNVLLSRDSPKWRQLKELHRETYNAVKKIYP
                     KLPVFFTTEVLHYKRLARDAKQSEQEKEVAELMRHSDLFAMSLYPYMSLETPHPLPDN
                     FLDFARRFKKPIAVSESGMTSRDVPLKSYGVSLLGSEAEQRNFTELLLKTASRDRYEF
                     VINFASIDSDRLVARLRPPLDDLARIWAFTGMQTSSGRPKPALPVWDGFLKAKYVR"
     gene            complement(8126..8455)
                     /locus_tag="VF182_03300"
     CDS             complement(8126..8455)
                     /locus_tag="VF182_03300"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEX6436130.1"
                     /translation="MKIVKIAAALKRLKKIGDEIRYLSCFEQKQFSSGLIVFKPSKKP
                     DPKQISHNDRDVICQVLQGCGRLRINGRKIRLAPGTLCHIPKKTPHDFAAGRSGELVL
                     LYLLIKT"
     gene            complement(8588..9562)
                     /locus_tag="VF182_03305"
     CDS             complement(8588..9562)
                     /locus_tag="VF182_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEX6436131.1"
                     /translation="MRLVTRADFDGLVCGALLTKLEAIDAYLFVEPKFMQDGLVEIRA
                     GDIIANLPYHPNCTLWFDHHITNTTPDFARPIVLGKGGFRLAPSAARVVYEYYKELAN
                     QPPIGSGEDKTTRRTKSEIIAFLDSERITYLLHETDRIDAGKLEPPDVLDPRGYVLIS
                     MTTDGRNAGDEPYWLRIIELLREASLEEILRDPEVSRRCQQIRDEQEELQKLLLARTV
                     MKGNVIYVDLRGIQDIPDGNRFLIFTLFPHGNIQVKVADDKQRENTTSISVGYNIFNT
                     TSNVNVGELLSHHGGGGHKVVGSCRVPNDQAERAIREIVAAVTDEEWQ"
     gene            complement(9559..10677)
                     /gene="ald"
                     /locus_tag="VF182_03310"
     CDS             complement(9559..10677)
                     /gene="ald"
                     /locus_tag="VF182_03310"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX6436132.1"
                     /translation="MIIGVPKEIKTEETRVAVTPTGVSGFTARNHKVLVEKGAGLGSA
                     LSDRAYQNAGAILVESAKQVWDESDLIMKVKEPQASEFPLLRPGLILFTYLHLAADEA
                     VTRALLDRKATAIAYETIQLDDGSLPLLTPMSEIAGRISIQVGAWCLQAENGGRGVLL
                     GGASGVRPGKVVIIGAGIAGTAACQVAIGVGADVSILDINPTKLRYVRDILGGHATTL
                     MSNRANLEEEVLEADLVVGSVLIPGAKAPKLISRSLVQRMKPGAAFVDIAIDQGGCAE
                     TSRPTTHRQPIYIEEEVVHYCVTNMPAIVPHTSTYALTNSTLSYGLELANRGFPQALA
                     RNKALAKGLNTFAGKVTHEGVAAAFNLALLPLEEVVRA"
     gene            complement(10913..11278)
                     /locus_tag="VF182_03315"
     CDS             complement(10913..11278)
                     /locus_tag="VF182_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEX6436133.1"
                     /translation="MERFFFAAGAAIAFLGVALGAFAAHSLKSRLPPDMLDIFEVGVR
                     YQMYHAFGLFVVAWASSRWPESNFNPAGWSFIVGIIIFSGSLYLLSLTGIRWLGAITP
                     AGGIAFLIGWAILIWTAGR"
     gene            complement(11340..12815)
                     /locus_tag="VF182_03320"
     CDS             complement(11340..12815)
                     /locus_tag="VF182_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436134.1"
                     /translation="MTIDGIRFVDTTIRDGHQSLWAENMTTGMMLPVAERMDRAGFDA
                     IELISSSHLKKCVRELKEDPWARVKLMSQRITRTPLRLNAGRFSAFDITPKSMYRLFM
                     ERMAANGIREARISEEWNELEGWRWKTEVARDVGINPVLNLIYSVSPKHSDQYFAQRT
                     RDAASLKVNRLCLKDPGGLLTPERVKTLVPIIFENAGGIPVELHTHCTTGLGPICCVE
                     AVKLGIKIVNTALPPLSDGSSNPSLFNVAKNLRALGYQTLIDEELLKPVSEHFTYIAK
                     REGFPIGEPLEYDYTQYQHQVPGGMISNLRHQLRKVGLEHKIDQALEETMQVRAELGY
                     PIMVTPLSQFVGSQAAINVIVGERYKEVTDQIIQYALGYWGREGGELLDAAIKAKILD
                     RPRAREWAQWQPPEPSVAEVRKKMNAEGVSDEELLLRWNLSVEEIDTMRAAGAPKEYL
                     TARQPLVKLIDALTKRTDYRQIVIQKGDMVVSLNRAAASAN"
     gene            complement(12930..13787)
                     /locus_tag="VF182_03325"
     CDS             complement(12930..13787)
                     /locus_tag="VF182_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6436135.1"
                     /translation="MARDIVAIDVHIHLSDETTQKAKGSRTAQMAKYFGRERPPVSID
                     ELADQYRARKMMAVLMNTRDETVTGLTPVPNDHVASAVKKHPDVFLAFGAIDPWTGKL
                     ALDEIRRCKDLGLHGMGELNPARQHFFANDVRFYPLWEECAKLNMPILFHGGMAAAGA
                     GTPGGMGIKLKYSQPIYLDEVAADFPELKIISAHPTWPWTAESLAIARHKANYFIDLS
                     GWAPKYFPAELLHNINTLLQDKAMFGSDWPAIGVERWLEEFQQVNMKPEVRKKIMLDN
                     AVKFFGLTL"
     gene            complement(13961..>15454)
                     /locus_tag="VF182_03330"
     CDS             complement(13961..>15454)
                     /locus_tag="VF182_03330"
                     /inference="COORDINATES: protein motif:HMM:NF014489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436136.1"
                     /translation="ISELQLRSPGGVMDTIHFVDTTIRDGHQSLWAENMTTGMMLPIA
                     KNLDRAGFEGIELISGSHLKKTVRELKEDPWERVRLMAKEITQTPLRVIAGRVNTFEY
                     NPPSMYWLFIERMFANGIREARISDEWNDYEGWKFRVGVCHAVGMKTILNLIYSVSPK
                     HSDEYFAERTRQAASLKPYRLCLKDPGGLLTPERMQTLVPIIFKNADGIPIELHTHCT
                     TGLGPLCCIEGMKLGIRSINTALPPLADDSSNPSLFNVAKNARALGYATAIDEEVLKP
                     VSKHFTAIAKREGFTIGAPVEYDYTQYQHQVPGGMLSNLRHQLRKVGLEDRFPEALEE
                     SIRVRAEFGYPIMVTPLSQFVGSQAAINVIVGERYKEVTDQVIKFALGHAGAEGARDM
                     DPNVKAKILDRARAREWARWQPDDPSAEEIRKRLDAAGVSDQELLLRWILGKEDIDAM
                     RANPRPLEYLNSEQPLVNLLQELAKRTGSRQIHVQTPAFSMTLEKRA"
BASE COUNT         3258 a         4198 c         4362 g         3636 t
ORIGIN      
        1 caatgcttct tccttatttt tcgtgtcgta gagccagttg gttgcctcga tcatggattt
       61 cagatagcgc acgacttcgt ttctgttcga cttcagataa ttcatgtgtc cgaaatccgc
      121 agtaaactga atcggaccga ggatctcatg gaaggtgata agttttgtga agccgtcttg
      181 ttgcgcccgg aagttgaacg gggggcccat gaaagtcgcc tgtaccgaac ctgatttcaa
      241 tgcggcgtag cgctccggcg aggtgccgac gaccaagtat cggtactctc ctggtttgag
      301 tttgaaacct ttgagtagga cttcttctat catgttggtc gtgccgccgg tcaaggtgct
      361 gacgccaatg gttttgcctt tgagctcggc gccatttttg atttcggttc gggccatcaa
      421 atcataggga attttgtcca tattcccgcc gatgatcctg accttggctc ccttttcaat
      481 ccctccgatc acgtaatccg gattgatgcg gccgagagga atggtttcgc tgagtacggc
      541 gcgggtagta ttggtggcgc cctgaatgat gatgttttcg aactggatgc cgtttttctc
      601 gaagattttc ttttcctggg cgatgtaaat cggccaactg gccgccgtgt gcccgctctg
      661 ggcaaatcgg agcttgctca actcggcgcc ggcgccgaaa gagatcgtgg ctgacgacat
      721 cgccaacagg agcgcgatgc cgagcgcgcg aatcttggtg ttatatctca tgcatcctcc
      781 ttaaatttgc gggtgtttac attagcgaaa aaccgccgga agcgtttacc tcttcctcga
      841 tttgtttcca taggatctgc tccagctcga gaaatcgagc gtctgacttc atctccatgg
      901 ttcttggacg cggcagcggc acattgactt cagaacggat gctgcccgga cgatggctga
      961 agacaatgac tcggtctgaa agaagaatgg cttcggcaat catatgggtt acaaagatga
     1021 cggttttttt agtctctctc cagatgcgca gcagttcgag ctgcatgaag ttgcgcgtct
     1081 gcgcatccaa tgcagcgaag ggctcatcca tcagcaaaat tctcggattt accgccagcg
     1141 ctctagcgag attgacccgc tgctgcatgc cgccggagag ctcatgtgga taatgagtct
     1201 cgaaaccgga aaggccgacc agatcgagaa atttctgcgc cgtccgttca gcttccttgg
     1261 agtcgtgatt ccgtccttcc aggccgagaa caacattttt gagactggtg cgccaaggca
     1321 ggagagatgc gccttgaaag acaaagccca tcagcagttc gttgtcgacc tcctgcgcgg
     1381 atctgccgtc gatgagaatt tctccaccgg aatgcggcat gagaccgttg ataacacgca
     1441 gcagggtggt cttaccgcag ccgctgggcc cgacgatgct gacaaattct ccatcgctca
     1501 ccgataggtt gatgtttcta aagatgacaa acgggtcgcg atccggacgg gtgatcgcct
     1561 tgttcaagtt gcgcaatacc agtgctgggc cggccatttc aacttccttg gggctctttt
     1621 ctccagggca gcgcatagat ttcgataatc cggatgattt cgaatcctgc tatcccgagt
     1681 gccgcaaata cggccacgcc gacgaataaa atgggtatgt tgagggtttg gccggcaaaa
     1741 aagatcagat agcccaaacc ctctctcgcg ccaaaccact ccgcgatggc gactccggtc
     1801 aggccgcggc cgacggacag acgtatgccg gccaatatga aaggaaccga cgccggcaaa
     1861 aggattttaa ggaacaatgc cgagcggctc aggcgaaacg agcgggccag ctcgacatag
     1921 tcgcgattaa ccacacgcat accgtaatag gaattgatca gaatgggaaa aaagcaaccg
     1981 agaaagacga tggcgatttt ggagccggcg ccgatgccta gccagagcag cagcaccggc
     2041 gcaaacgcaa ttcgcggcgt cgtataaagc atcgacatcc acggattgac gtaatcgcgc
     2101 gccaccgtat tgagcgccat cagaaaaccc aacggcacgc cggcgagaat agccagcacg
     2161 aagccataga aaaaaatcca tgagctggcg aatatatgag ggtagaggtc cttagtgacg
     2221 aagagcatct gataggcggt gacggctacc accaccggcg ataccagcag ggttgggtct
     2281 ttcacaaccg cggtggccag aacatgccaa agaagcagtc ctccgatgac ggagcccgcc
     2341 tttaggaaag tggacgtcaa tttgaggttg cgtactttca tatcgtagcg acgtctgcaa
     2401 tcactccgaa gaccattcgg cgctctgacg ccagggcgcc atccgctgtt cgagtttctt
     2461 gatcagatta aaactcacaa gtcccatggc ggccagcaat atcacggtga cgaagagctt
     2521 cggactgtcc caggtttccg aggaatcgcg gagcagaaat cccaagcccg cattagcggc
     2581 catgagctcc gcgacgatga ctcccagcag cgcacggcca actccgagtc tcatgccggt
     2641 gatgatgaag ggcagagctc cgggtaagcg gattttgtaa ctcgtctgcc agtcgctcag
     2701 ccgcagcgat ttggctaact cgagccatac tggatccagc gatttaattc ccgcggcggt
     2761 gttcagaata attgccaccg cagtgatctt aaaaactact acgactttgg aaaagatccc
     2821 gataccgaac caaatgatga tcagcggcac gacggtaata atcgggatcg agtaaagcgt
     2881 cgctatccac ggatccatga tttcatcgaa ccatttatac attcccatca aatagccgag
     2941 aatgatcccg atgaagcaag cggtggtaaa gccgcagata aattcgatga gagttgcgac
     3001 aaggtgccgg ttgagctcgc ccgtgctgag gagattccag aacgagatta gcacgctgct
     3061 tggcggcggg atgagtagtt cattatcgag cagcagacga gtcgacgctt cccagatcgt
     3121 caggccgatg atgatcgaca aagatccccg gaaaatttct tttttaggca agagccggct
     3181 catgctactt ataaagctct tgatcttcgc cgttgtcgtc gcgcatgtgg attttaggat
     3241 agaccgagac gccgcccggc ctgccttgca ccatgaaatc gaccgggatg aagccctgca
     3301 acaaaggtat cggtaattgt actccggcaa acgatccctt gtgatcgccg ccgataccga
     3361 tctgatttcc ccagagtccg gcaggtaagg cgccgctcat gctgaaacgg ctggcggaaa
     3421 cacttccttg aatcggcggc aaggaatact tgccggcggg gtcgcgctcg ttttcactga
     3481 gcatttcatg atccagaacc ttgggaaatg caggggcgtg agagctggcc tcgcgcacga
     3541 aagaaaaggt cgtgtcctca tgctcttgtt cagactcgcc ggcgtttggg cgcgcgcctt
     3601 ggatgatcgg catcggcaga tttttgatgc cgccaatgct gcctttgccg cgctggccgg
     3661 catcggccaa tgcgaccgca tggttttttt gccgcagcgc gacaaaaaaa cgttcggtgg
     3721 tctggcgcgg caacgagtag ctttggctgc gcacaccgga aggcgtctga taatagatca
     3781 gaagctgatt atcaccggtg atagcgacgc cgcgatccgc caattccatg ttccgtgtgt
     3841 tgccgtcgaa caacgatgcc ttgaggccga gcccggtcgg gccaaagagt tccggatttt
     3901 ctttcaaaaa ttctttgctc atacgcaaac cgctgttatc ggtcttagcc agttcggcga
     3961 gttttttggt ggcgtctgcg gccgccgggc tttctggata gtgatcgagc agggtcgtca
     4021 gataatactc ctgagttcct ctccctttgc tcttggttgc cgcgttcagc agcgctgttg
     4081 ccgccttctg ctttagtgat gaaagctttt ccgcggacgt tccggcaagc tcatgatagg
     4141 cgatcgcttt atcgatcatg cctttttctt cataagtatt ggcaagaatt ctgtaaactt
     4201 cggtggcgct gactgaatcc gggtgactgc ggacgtagga gacgccggcg tcgatcaccg
     4261 attgcgccga aaccggattg ttgcttagca cattaataag attgttaccg accatcatcg
     4321 cattggcggc gccgagagtg acggctccgg ccggaccggc tgcggcgacg gcgccggcgg
     4381 cataaatcag gttcttttta agcagatcct cgcccagaag gacatacttc accgactcgg
     4441 cgcgtcgttc gctgcgcgcc gcctcgaatg tggcgagcag attgtactcg gggctttgta
     4501 acagcgccgc ggcgtgtttt tgcgccgccg gagtctttgc cgacgaggcg agccgctcga
     4561 taattttttt tgcctcttca tggcggcctt gcatttccag agctaccgat gacgcatccc
     4621 gggcggaatc ggccagcggc ttgccggcat agtttttctc cagagtggcg gcttgctgcg
     4681 cgatttgctg ttgatctctc aagctcagcg ccagcagcag ttcttcgaca tcctcctgtt
     4741 gctcggtcga accgggcttc tcggcttgtg ctgaaagacc ctggtattgc tgctcgctac
     4801 gatgcaggta ggacttcgag agcttctgca gctcggcttg gactattctg gatcgaccat
     4861 ccaaatagga cgcgacctcg gcatagaaga aggccttgtc tatatctccc ttttgcgccg
     4921 cttgctttga atgatccagt tgtttgcgaa tcagcgcggc ggtttttttc ttttcgagct
     4981 cttcgatgtc cttgacgatc ttgccattgc gcggatcgtt gggatagcgc ttgagatgat
     5041 ccatgtggcg cgtcaaagcg cgccgctctt caaccgacat ctcagtgttg aggagcgagt
     5101 aggcctgtcc aatggcggtt tccgcggcgg cgccaatgta atttcccgaa gccacgccga
     5161 tcaggtctac cgagctcaac aaccggttga atacgccacc caactcattg accgagcttt
     5221 gccgggtcaa ttgatcggct cgtgtcagat catcttcatc gataatcgct tgcagatatt
     5281 ttttgcgttc cggcgacgag gtattcttca gagcctggcg cagcgccgcg agataacgct
     5341 ggcgctcatc aaccgtattg ttcagcagat cctgggcgtg gtcggatagt ccggtgctcg
     5401 atccgtgttg tttctgtagc cggcggtctt cggtctgaaa aaactccgcg tgctctgcga
     5461 tcgctgcttt gtcgttggta agcgaatcga tcagcagttc tcgcgcgcgc ttgtcgactt
     5521 catcggggaa aaactttggc gcttgcggcg gcttttcgaa tggatcgaat tcaggcagag
     5581 gattcaaaat gcgctcgatc atcgcttgcg agtcctgccc ccgcacaggg tcggggtaac
     5641 cgtcgatggc gccgagcgcc agcgacaacg acaatgcgct tatccatgat tttctcatcg
     5701 gcttagcctc gtgactttct cgatcaggcc ccgcattgct tcaatgttgc cttgcgcttc
     5761 tatcttttcg acgatgccat cgacttggac cacttctgtg taaagtttaa gcgccgattt
     5821 agcaaattga tcgaacgcct tgagttcaaa gcttaaaccc tcaggatcgt actgggcggc
     5881 gtactctttt gcaagcaatg cgtagaagtc gccaaagtcg atgatgaacc gatagatatt
     5941 cttgctttgc cggtgtttgg tgatcaactg gccgtaaccc agtatgacca gttgattgcc
     6001 gtccttgagc cggtagcgat tggcttcgac gaacgccact ttagcgcgat cgatccgttc
     6061 ctcttcgcag cgggcgagaa attcataagt agtgccttga tgtttttcaa tcaggtgatt
     6121 ccaggcggcg actttttcgt ccaggccttt gatgtactgg aatggatctt cggtgggcag
     6181 gggcttgtcg aggatgttca ggaaagattc cagggcttcg aggttcttgg cagcttcggc
     6241 tcccaagcgc ccgccgattt cgctcacgtt acggtacaga acggtggcat tggcgtactc
     6301 tcggagtcgc tccagcgcca gcgctttgtt gaattgaatg atgtcggtga acttgcccgc
     6361 attctttttt tcatagtcat cgagccggac caaggtggcc ttcatgatgt tcactccggt
     6421 aacgtctttg ggtatggcaa aacggtaggt atcttccttg gccagacgct gaaagtcttt
     6481 gacaatatcg agcaggtctc ccgccgccgc atagcgcgct tcaggcgaag cgattcgcga
     6541 tgaacatccg gcaaggatca atagagagag aaccgtcgtg acggagatca cggagcggaa
     6601 tagtagtcgg aacgatatca aaatattctt tgcacagttt cttcgatgac gggcgccgag
     6661 agatgttgtt cagagtctga cggttgcagt tattttccga agttttcttc ctaagtcaag
     6721 cgagggtaac gcggctcggc tccgttctcg cgcccagatc atccggcgct ttatccggta
     6781 cgcgagaacc ggcgcgcaaa tgctgcaacc ctccgcctct acttgaccgt ctctgcgcaa
     6841 caaatcatag tgatggctat gaaagttttt cttcaactgc ttgtcttgcc gatcctcttc
     6901 gcaagtttca tcgtcggctg cgtgcctgcc gctcacgagg aaagcggcag cgcgcccagg
     6961 gatccggggg ctaccgctgc cgcctccttg cctgagtatc agggccctgc tacgcggccc
     7021 tttgtcatgg gattcacgcg atggccggcg gatctgacgc cggagggagt agcggccgcg
     7081 cagaagtttg ccgatctcca cggcgacata atggctgtga tgttcattgg cggaattcca
     7141 tggcctgaag cgcttgaagg caaaccctat cctaaagatg ttcaggacgc tctcgccgac
     7201 aggccgccga gcgaaaagaa gctgttcttg tcgatctcgc cgctggacag agatcgcaaa
     7261 ggcctggcgc cgtactgggg agaaaaggag aacgagccgt tgcccaagcc gtgggacaaa
     7321 gaaccgctca atagccctcg ggtcaaaaag gcttttttga acttcgtgct gcattctgtg
     7381 aaagccatgc ggcccgacta tttggccatt ggcgtggaat cgaacgtgct gctctcgcgc
     7441 gatagcccca agtggcgcca gctcaaggaa ttgcaccgcg aaacttacaa cgcggtgaag
     7501 aaaatctatc ccaagttgcc ggtatttttt accaccgagg tgctgcatta taagcgcctg
     7561 gcgagggacg ccaagcaatc ggagcaggag aaggaggttg ccgaattgat gcgccacagt
     7621 gatctcttcg ccatgagcct ctatccctat atgagtctgg agacgccgca cccgctgccg
     7681 gacaattttc tcgatttcgc cagacgattc aagaaaccga tcgcggtctc ggaaagcggc
     7741 atgacgtcgc gcgatgtgcc gctaaaaagt tacggcgtca gccttttagg ttcggaagcc
     7801 gaacaaagaa acttcaccga gttgctgctg aaaaccgcca gccgcgacag atatgaattc
     7861 gtcatcaact ttgccagcat cgattccgac cggctggtcg ctcgtttgcg tccgccgctg
     7921 gacgatctgg cgcgcatctg ggcattcacc gggatgcaga caagcagcgg aaggcccaag
     7981 ccggcgctgc cggtctggga cggctttttg aaggcgaagt acgttcgtta acgaggagag
     8041 attccggctc gttagacggt gtcattcgta aacagtcgcg ttgaccggcg gccgaatttt
     8101 cgagaccagc ttcctagttg tacaatcagg tcttgatcaa tagatacaaa agcaccagtt
     8161 ctccgcttct gccggcagcg aaatcgtgcg gcgtcttctt ggggatgtga cagagagtgc
     8221 ctggagcgag tcgaattttc cggccattga ttcgcagtct gccgcagccc tgtaatacct
     8281 ggcaaatgac gtctcgatcg ttgtggctga tctgctttgg atcgggtttc tttgacggtt
     8341 tgaaaacgat caggccactg ctgaactgtt tctgttcgaa gcagctcaga tagcggattt
     8401 catcgccaat cttttttaac cgcttgagcg ccgcggctat tttgacgatt ttcatcgggg
     8461 ctagggctat gactattggc ccacaggcaa tagccagcag tctttgaaac aatgcgagcg
     8521 agattcgact caggaatttt ggctcggtta attgcagcga atcctactgt agcctgttgc
     8581 ctaacggcta ttgccattcc tcatcggtca cagcggcgac gatttctcgg atcgctcgct
     8641 cggcttgatc gttggggacg cggcaggagc cgacgacttt gtggccgccg ccgccgtgat
     8701 gacttaacag ttcgccgacg ttaacgttgg aggtggtatt gaagatattg tagccgacgg
     8761 aaatgctcgt ggtgttttcg cgctgcttgt cgtcggcgac cttgacttga atattgccgt
     8821 gcgggaacag agtgaagatc aaaaaccgat tgccatccgg gatatcttgg atgccgcgca
     8881 gatccacata gatgacgttg cctttcatga ctgtccgcgc cagcaaaagt ttttgtagct
     8941 cttcttgctc atcacggatc tgctggcaac gccggctgac ctcggggtca cgcaggattt
     9001 cttcgaggga agcctcgcgc agcaattcga taatccgtag ccagtagggc tcatcgccgg
     9061 catttcgtcc gtcggtggtc atggaaatca gcacataccc tcgaggatct agaacgtccg
     9121 gcggctccag cttgccggca tcgattcgat cggtttcatg gagcagatag gtgattcttt
     9181 cgctgtcgag aaacgcgatg atttcgctct tggtacggcg ggtggttttg tcttcgccgc
     9241 tgcctatcgg cggttgattg gctaattctt tataatactc gtagaccacg cgcgcggcgc
     9301 ttggcgccaa gcgaaagccc cctttgccga gcacaatcgg tctggcaaag tccggggtgg
     9361 tgttggtgat gtgatggtcg aaccagagag tacagttggg atgatacggc aggttggcga
     9421 tgatgtctcc ggcgcggatt tcgaccaaac catcctgcat aaacttcggc tcgacaaaga
     9481 gataggcatc gatggcttcc aatttcgtca gcagcgcgcc gcagaccagc ccatcgaaat
     9541 ccgcgcgagt gactaatctc atgcgcgaac gacctcctca agcggcagca aagcaagatt
     9601 gaaggcagcc gcgactcctt cgtgcgtcac cttgccggca aacgtgttaa gacctttggc
     9661 caaagctttg ttgcgcgcca gtgcctgcgg gaagccgcga ttggcaagct ccaggccata
     9721 cgacagggtc gaattagtca atgcataagt cgaggtgtgc ggtacgatgg ccggcatatt
     9781 ggtcacacag taatggacga cttcttcttc gatatagatg ggctgacggt gtgtcgtcgg
     9841 ccgtgaagtt tcggcgcagc cgccctgatc gatggcgatg tcgacaaagg cagccccggg
     9901 cttcatgcgt tgcactaacg atcgcgaaat aagtttgggc gccttggcgc ctgggatcag
     9961 cactgagccg actaccagat cggcctcaag gacttcttcc tcaaggttgg cacggttgga
    10021 catcaacgtc gtcgcgtgac cgccgagaat atcacgaacg taacgcagct ttgtcggatt
    10081 gatgtcgaga atgctgacgt cggcgcccac tccaatcgcg acctggcaag ctgccgtccc
    10141 ggcaatgccg gcgccgatga tgactacttt gccgggccgg accccggaag cgccaccgag
    10201 aagaacgccg cgcccgccgt tttccgcctg caggcaccac gcgcctacct ggatcgaaat
    10261 tcgtccggct atttcgctca tcggcgtcag gagcggcaac gagccatcgt ccagctgaat
    10321 agtctcataa gcgatggcgg ttgccttgcg gtcgagcagg gcgcgagtta ccgcttcgtc
    10381 ggcggcgaga tgaagatagg taaaaagaat cagcccgggc cgcagcagcg gaaattccga
    10441 tgcctgcggt tctttgactt tcatgatcag atcggactca tcccagacct gttttgctga
    10501 ttcgacgagg atcgcgcccg cgttctggta ggcgcgatcg ctaagcgcgc tccccagacc
    10561 cgcgcctttt tcgaccagga ccttgtgatt gcgcgccgta aaaccgctga ctccggtggg
    10621 cgtcacggcg acgcgagttt cctcggtctt aatctctttg gggacgccga taatcatctg
    10681 ggtgaaagct actcgatggt atctggagag tcaacgtgaa ggctgctgaa aaggctcata
    10741 tgccttcgga aaggatgaat tatgaaagct gaaaactgaa ttccgctttc atccttgtgc
    10801 tttgcgcccc gcctctgaat ccttttgagc ccctttttga gtcctgctcg agtaaggcgc
    10861 tcggataggg cgtcatactt tgatttttga acaaatgggc tgttgggttc gactatcgcc
    10921 cggctgtcca gatcaggatc gcccagccga tcaggaaggc gatgccgcct gcgggtgtga
    10981 ttgcgccgag ccagcggatc ccggttaagc tcagaagata aagactgccc gagaagataa
    11041 tgataccgac gataaacgac cagcccgccg gattgaagtt gctctcgggc caacgcgatg
    11101 acgcccaggc gacgacgaac agtccgaagg catgatacat ctgataacgg actccgactt
    11161 cgaagatgtc gagcatgtca ggcggcaggc ggcttttcag cgagtgggcg gcgaaagcgc
    11221 caagcgcgac gcctaagaag gcaatggccg cgccggcggc gaagaagaag cgttccataa
    11281 aatgagtgcc ttggtcgtga aacgtgatcg tgttcgtgga aaacagaaaa tgaagatatt
    11341 taattcgcag aagccgccgc gcggtttaat gaaacaacca tatcgccttt ttggatgacg
    11401 atctgacggt aatccgttcg tttggtcagc gcgtcgatca gctttactag cggctgccgt
    11461 gcggtcaggt actcctttgg ggcgccggcg gcgcgcatcg tgtcgatttc ctcgacactc
    11521 agattccaac gcaacagtag ctcttcgtcg gacacgcctt cggcgttcat cttcttgcgc
    11581 acctccgcta ctgacggttc aggcggctgc cactgggccc actctcgggc gcgcggtcgg
    11641 tcgaggatct tggctttgat cgccgcgtcc aacaactcgc cgccttcccg gccccagtaa
    11701 cccagcgcgt attgaatgat ttgatccgtg acttccttgt agcgctcgcc gacgatcacg
    11761 ttgatggcag cttggctgcc gacgaattgc gagagcggcg tgaccatgat cggatacccc
    11821 aattcggcgc gcacctgcat cgtttcttcc agcgcttggt cgatcttgtg ctccaagccg
    11881 actttgcgca gctgatggcg taaattggaa atcattccac ccgggacttg atgctggtat
    11941 tgggtatagt cgtattccaa cggctcgccg atcggaaacc cttcgcgctt cgcgatgtaa
    12001 gtgaaatgtt ccgagaccgg cttcaaaagc tcttcatcga tgagggtctg ataacccaac
    12061 gcgcgcagat ttttggcgac gttaaagagc gaaggattcg atgatccgtc cgataacggc
    12121 ggcagagctg tgttgacgat cttgatgccg agcttgaccg cttcgacaca gcagatcggc
    12181 cccaatccgg tggtgcagtg cgtgtgcaac tcgacgggaa tgccgccggc attctcgaag
    12241 ataatcggca cgagagtttt tacccgttct ggagtgagca agccgccggg atctttgagg
    12301 cacagccggt ttactttgag cgaggccgcg tctcgggttc gctgcgcgaa atattgatcg
    12361 ctgtgcttgg gcgatactga atagatcaga ttgagaaccg gattgatgcc gacgtcgcgc
    12421 gcgacctcgg ttttccagcg ccatccttcc agctcgttcc actcttcgga aatccgcgcc
    12481 tcgcgaatac cgttcgcggc catgcgttcc atgaacagcc ggtacatcga tttgggcgtg
    12541 atgtcgaagg cgctaaaccg cccggcgttc aagcgcagag gcgtgcgggt aatgcgctgg
    12601 ctcatcaact tgacccgagc ccacggatct tccttaagct cgcgcacaca tttctttaga
    12661 tgggaactgg agatcagttc gatggcgtcg aagccggccc ggtccatgcg ctcggccaca
    12721 ggcagcatca tgccggtcgt catgttttcc gcccacaggc tctgatggcc gtcgcggatg
    12781 gtggtatcga cgaatctaat tccgtcaatc gtcacttgat cagaatccct caaagacttt
    12841 tcaccgcaga ggcgcaaagt acgcagagaa ttagaagcga taactcttcc tctgcgctct
    12901 cggcgtctcg gcggtgaatc ctggtttttc tacagcgtca agccgaagaa cttgactgcg
    12961 ttgtcgagca taatcttctt gcgcacttcc ggtttcatat tgacctgctg aaattcttcc
    13021 agccagcgct caacaccgat ggccggccaa tctgagccga acatcgcctt gtcctggagc
    13081 agggtattga tgttatgcag tagctcggcc gggaaatact tgggcgccca gccggacagg
    13141 tcgatgaaat aattggcttt gtggcgcgcg atggctaagc tctccgccgt ccagggccag
    13201 gtcggatggg cgctgatgat cttcaactcc ggaaagtcgg cggcgacttc gtccaggtaa
    13261 atcggctggc tgtatttcaa tttaatgccc atgccgcccg gcgtgccggc tcccgccgcc
    13321 gccatgccgc cgtgaaacag aatcggcata ttgagcttgg cgcattcctc ccaaagcgga
    13381 taaaagcgaa cgtcgttggc aaaaaagtgt tgccgcgccg gattgagctc gcccattcca
    13441 tgcaggccca gatctttaca gcgacggatt tcatccaagg cgagcttgcc ggtccaggga
    13501 tcgatggcgc cgaaggcgag gaacacgtca ggatgtttct tgacggcgct ggcaacatga
    13561 tcgttgggca ccggggttaa accggtgacg gtctcgtccc tcgtgttcat cagaaccgcc
    13621 atcattttgc gcgcgcgata ctgatccgcc aactcgtcga tggaaaccgg cggtcgttca
    13681 cggccgaagt atttcgccat ttgcgcggtg cgcgagcctt tcgctttttg tgtggtttca
    13741 tcggaaagat gaatgtgaac gtcgatagcg acgatgtctc tagccataga gtggtttcct
    13801 ccttattttt gccgttaaga gcggctgcag aatttattat ccatccttat tgcatcggct
    13861 gacctttagt tcaaggttcg atgttcaagg tttaaatatg ggactggttc ggtgtatgcg
    13921 aatcgttgac cttgaagtgt cgaacgttga actttcgcgc tcatgcgcgt ttttccaggg
    13981 tcatcgagaa cgcgggtgtc tgaacgtgta tctgccgact gccggtgcgc ttggccaatt
    14041 cctgaagcag gttgaccagg ggctgttcgg aatttaaata ttccagcggc cgcggattgg
    14101 cgcgcatggc atcgatgtcc tctttgccga ggatccagcg cagcagcaac tcttgatccg
    14161 agacgcccgc tgcatcgagt cttttgcgta tctcttcggc gctcggatcg tcgggctgcc
    14221 agcgcgccca ctctctggcg cgcgcccggt cgagaatctt agccttgacg ttagggtcca
    14281 tatcgcgcgc gccttcggcg ccggcatgac cgagggcgaa cttgattacc tggtcggtga
    14341 cttccttata gcgctcaccg acgatgacat tgatggccgc ttggctgccg acgaattgcg
    14401 atagcggggt gaccatgatc ggatagccga actcggcgcg aactcgaatc gattcctcga
    14461 gcgcttccgg gaagcgatct tccaatccga ccttgcgcaa ctgatgccgc aggttggaaa
    14521 gcatcccgcc cggcacctga tgctggtact gggtatagtc gtattccacc ggcgcgccga
    14581 tggtgaagcc ttctcgctta gcaatcgccg tgaaatgttt cgataccggt tttagcactt
    14641 cttcgtcgat ggccgtggca taacccaacg cccgggcatt cttggcgacg ttgaacagcg
    14701 aaggattgga agagtcatcg gccagcggcg gcagcgcggt attgatggac cgaatgccca
    14761 acttcatgcc ttcgatgcag cacagcggtc ccaaaccggt cgtgcagtgc gtatggagct
    14821 cgatgggaat gccgtcggca ttcttgaaaa taatcggcac cagcgtttgc atgcgctccg
    14881 gagtcaacag cccgccggga tctttgaggc acaggcgata gggtttaagc gaagcggctt
    14941 gccgcgtccg ctcggcgaag tattcatcgc tgtgcttggg cgaaaccgaa tagatcaggt
    15001 tcaaaatagt cttcatgcct acggcatggc agacgccgac tctgaacttc cagccttcgt
    15061 agtcgttcca ttcatccgag atccgcgctt cgcgaatgcc gttggcaaac attcgctcga
    15121 tgaagagcca gtacatcgac ggcggattgt attcaaaggt gttgacccgc ccggcgatga
    15181 cacgcagtgg cgtttgagtg atttcttttg ccatcaatct aacccgctcc cagggatctt
    15241 ccttgagctc acgcacggtc ttcttaaggt gcgacccgga aatcagctcg atcccttcga
    15301 agccggctcg gtccagattc ttggcaatcg gcagcatcat gccggtggtc atattttccg
    15361 cccacaagct ctgatggccg tcgcgaatcg tggtgtcgac aaagtgaatg gtatccataa
    15421 cgcctcccgg cgatctcaat tgaagctcac tgat
//
LOCUS       DASUVS010000058        10707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_239012, whole genome shotgun sequence.
ACCESSION   DASUVS010000058 DASUVS010000000
VERSION     DASUVS010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10707
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239012"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VF182_03335"
     CDS             complement(<1..385)
                     /locus_tag="VF182_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEX6436137.1"
                     /translation="MNTAVVDVIEQKRLNEAREAQIPWKKWGPYLSERQWGTVREDYS
                     DNGNAWDYFSHDQARSRAYRWGEDGLGGLCDEKQRLCFALALWNERDPILKERLFGLT
                     NSEGNHGEDVKEYYFYLDSTPTHSYM"
     gene            652..1092
                     /locus_tag="VF182_03340"
     CDS             652..1092
                     /locus_tag="VF182_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6436138.1"
                     /translation="MNKDDVIGNTYLFRGITADDFSALARIVEQVVFLPGHLVYDVDQ
                     VSDAIIVIQMGTVDIVVKGKQAPIVTMGNGQTLGELAFFQPGKRFATATARERTEVAR
                     IPFDKLRELFAERPTLALLFYQNACTFLAKHVRELASERDRRYF"
     gene            complement(1195..2589)
                     /gene="zwf"
                     /locus_tag="VF182_03345"
     CDS             complement(1195..2589)
                     /gene="zwf"
                     /locus_tag="VF182_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX6436139.1"
                     /translation="MSNAAPADALVFFGATGDLAHKKIFPALQALAQRDNLDIPVIGV
                     AKAGWTLDQLKARARDSVERFGGLNPDAFAQLSRCLRYIDGDYGDAATFQQLRRELAD
                     AKHPLHYLAIPPGLFDIVIHQLGSSGCARGARVVLEKPFGRDLATAQKLNLVVHSHFS
                     EENVFRIDHYLGKNTVQNLLFFRFANSFLEPLWNRQFVESVQITMAESFGIEGRGKFY
                     DQTGAVRDVVQNHLLQVLSNIAMEPPPGLDAEMLRDEKAKVLKGIRPLQPRDVICGQF
                     RGYRDEPGVTVNSRVETFVALRLEINSWRWKGVPFYIRAGKCLPVTATEVLVKLRQPP
                     AVFSEVAPPANYFRFRITPDLMIAVGALVKKASDQPEGQHVELVISEESDPAEMGAYE
                     ELLFDAMRGNSARFARQDYVEQAWRIVDPILGDVDPVYEYEPGTWGPPEVSGLIEPEG
                     GWFNPNFRQRPVKI"
     gene            complement(2941..3441)
                     /locus_tag="VF182_03350"
     CDS             complement(2941..3441)
                     /locus_tag="VF182_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436140.1"
                     /translation="MMPVIILLVGCSSAPPVAFQNYSHRQLHSLRLEDLQQVQFYLSN
                     DVVAQYKDASGTKSLLLPRLTPGVATSEGPNWIKVSFREGGVDVPFVTDPKQSDGRYY
                     IATEVAGSKEFKRISDLPVRSFLYKGVQYNVASGGDAILLFDWQSWNRVVETRKTTGG
                     RQVGGK"
     gene            complement(3700..4854)
                     /locus_tag="VF182_03355"
     CDS             complement(3700..4854)
                     /locus_tag="VF182_03355"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HEX6436141.1"
                     /translation="MGTAHAAAPLRADPKSLERGTPELIKRLRADSYNYFRFVNRSWI
                     ARVCDDFAGDLEGLPVIRLHGDAHVEQFALTQDAWGLDDFDDSASGPAVVDIVRFLGS
                     VELVARRRSWQQDRDRLFDRFVEGYKRGLIEPDHLPPPPDIVGRLRTAAPATRAALLA
                     WGESKMQPLADASMKAVAGGMEAFAQIMLRERRDLGTDYFRIIRAGSVQSGVGSAVTL
                     KIMIRVRGPSDDPADDELLESKKIGDLGGLSCLKTPMVNPTLRIIDGSKQLGRLRHNI
                     LAAGPELMVPEVMARGERLQDWWIRSLDPSYSQIRLTDLRSVGDLAAISYDAGVQLGA
                     GRLQDRTVLLSTYDRKRLLGATGKLEKRYRQKATQLVDDLLQGWREFAQR"
     gene            complement(5102..6805)
                     /gene="aspT"
                     /locus_tag="VF182_03360"
     CDS             complement(5102..6805)
                     /gene="aspT"
                     /locus_tag="VF182_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0070906 - aspartate:alanine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-alanine antiporter"
                     /protein_id="HEX6436142.1"
                     /translation="MDYLIKALQQNPELAIFLALAIGFAIGRVKLGSFSLGTVVGTLF
                     AGVLIGQFDVKVPALVKTVFFDLFLFTTGYKVGPQFFRGLKRDALSQVTLTLVLCTTC
                     LAAALIAAKLFDYDIGTAAGLLAGAFTESTVIGTAGDAINRLAISAEEKAHLLNNIPV
                     AYAVTYLVGTAAVVWFLPKIGPKLMGVNLQEEAKKMQAQVSGESEEQPGIISPSRAFD
                     VRVYCVTNEKLANKSIAEIEAMPQEVRVFIQRIRREGAIIEAEPTTIIHRGDVIAVMA
                     RAEILVERSSVVGPEVTDKQLLDFPLEVLDVVVTNKDLVDKTFKELWTSEPWRMHFRG
                     VFVRKLLRAGAEMPIAMTTQVERGDLLSLVGAKRDIDRAVAAIGYADRSSNATDMIFV
                     GTGIFIGGLVGLLTVTIAGLPITLTASGGALIMGLVFGWLRSVFPAFGRIPEAAMWVF
                     DTVGLSVFIGVVGLSAGPSFIAGLQKSGISLVFVGLVAAVLPHIVTILFGRYVLRMNP
                     VILLGACSGAGTMTAALRAIQDEAQSKLPALGYTVPYAIGNIVLTAWGPVIVALLSIG
                     K"
     gene            complement(7131..8543)
                     /locus_tag="VF182_03365"
     CDS             complement(7131..8543)
                     /locus_tag="VF182_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEX6436143.1"
                     /translation="MVSTQVRIEHDFLGDREVPADAYYGVQTLRGKENFHITGMPISR
                     EPHFVKAFGYVKKAAAMANRDLGVLDAKIADAIIRACDRLIAGEMLDQFVTDFIQGGA
                     GTSTNMNANEVIANLALEILGHEKGEYQYVNPNDHVNFGQSTNDVYPTAFHLGLILRL
                     ESYMEALRQLQAAFFAKAREFDKVLKMGRTHLQDAVPMSLGSEFHGWGTTIGEEVERL
                     ASVRQMLQCVNLGATAIGTTVTAAPGYPALATKHLSDITGIQFCLAEDLIEATSDTGD
                     YVLLSGVIKHTAAKLTKISNDLRMLASGPRCGLNEINLPQMQPGSSIMPGKVNPVIPE
                     VVNQTSFLVIGLDVTVTLAASAGQLQLNVMEPVITFALFASIATMERAVNSLRVHCVE
                     GITANAERTRDMVLNSLGIVTLLKPSLGYRQCAELAREGYETGKSLHDIVVTEKKLLS
                     QQRWDEIFSFENLINPKFEQ"
     gene            complement(8573..9220)
                     /locus_tag="VF182_03370"
     CDS             complement(8573..9220)
                     /locus_tag="VF182_03370"
                     /inference="COORDINATES: protein motif:HMM:NF016704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6436144.1"
                     /translation="MHAIDYLVHFSNILMLAAYSVRDILWLRWFAVAAALTNIPYFLL
                     QGTVLWPPVLWALVFTAINLYQIARLYLERRPVVLSQDEQKLYDLGFRSLRPREFVSL
                     SLVGEWKNGEPGERVLTEGEPVSSLCIPITGSADVHKHGERIGALTPGHIIGTALALT
                     GEPSPVEVTFTEPARYMRWSLPSLRRFMDARPDLRVTLQGLVNRDLARKLERALS"
     gene            complement(9241..9948)
                     /locus_tag="VF182_03375"
     CDS             complement(9241..9948)
                     /locus_tag="VF182_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436145.1"
                     /translation="MIQVFLANRFHGTILMFAAALLAGCTPLYLSDTHTTSTPRSELL
                     DVAALEREPVATLGLLAPAGLQGLNPPLSHALNAALSEASPPIRGIAAHETVNTINEQ
                     GLAGEYGTLMAGFGRGGILERKPLERIGSALSSRYVLLPGLAEFNQVIIDRLSISGWN
                     FIQSRVTTMRLWLQLWDTQTGKILWESAGEVTVATKLLRSEQTVPLDGIAQKLWLRMI
                     QDNLLEGKTGSRFFFSN"
     gene            complement(10048..>10707)
                     /locus_tag="VF182_03380"
     CDS             complement(10048..>10707)
                     /locus_tag="VF182_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEX6436146.1"
                     /translation="VQSWAATELNKMGALSKREITLAVLVLLALVMWIFRGDEVNATT
                     AALIVISLMLILNVVTWDDIVKNSAAWNTLAWFATLIALADGLTRVGFVKWFAETVAG
                     QLTGVTPAIAVAALLFINFFGHYLFASVTAHVTAMIPVLLAVASTIPGVNMHTLALGM
                     CLQLGIMGIITPYASGPSPVYYGSGYLPSADYWRLGAIFGVIFFAVFLVIGVPWMLAI
                     R"
BASE COUNT         2335 a         3090 c         2981 g         2301 t
ORIGIN      
        1 tcatgtacga atgagtgggc gtgctgtcga ggtaaaagta atattctttg acatcttcgc
       61 catggtttcc ctcgctgttg gtgagcccga acaaacgctc tttgaggatc ggatcgcgct
      121 cgttccacaa agccagcgca aaacacagcc gttgcttttc atcgcagagg ccgccgaggc
      181 cgtcctcgcc ccaccgatag gcgcgtgagc gcgcctgatc atgggaaaaa taatcccacg
      241 cattgccatt atcgctgtag tcctcacgca ctgtgcccca ctggcgctcg ctcaggtacg
      301 gtccccattt cttccagggg atttgcgcct cgcgggcttc gttcaaccgt ttttgctcaa
      361 tgacgtcgac gacagcggtg ttcatggttt acccttcctg catcaccaaa agtgatctgt
      421 tcaatccact ccagcaacga atgttgatcc ttcttctaac aagttagcaa aagcgcaggt
      481 attggacttt ggtccaattg attgccaggt ttggggagca tatggtttgt ttcaaacgtt
      541 gcaacgtcga ggagaggacg gatatagtcg acgtcatagg tgaatggtct ggtattgaga
      601 aattctctta caaatccgat tactcaatac aaactagagg gtgacgaact gatgaataaa
      661 gacgacgtta tcgggaatac ttacttgttt cgtgggataa ccgccgatga tttctccgcc
      721 ttggcgagaa ttgttgaaca ggtggttttc ctgccgggcc atcttgttta tgatgttgat
      781 caagtttccg acgccatcat tgttatccag atgggcaccg ttgatattgt cgtcaaggga
      841 aaacaggcgc cgatcgttac gatgggaaat ggccagacct tgggcgagct cgcttttttc
      901 cagcccggga aacgatttgc gactgccacc gcgcgcgagc gcactgaggt cgcgcggata
      961 ccttttgata agctgaggga gttatttgca gaacggccga cgctcgcgct acttttttac
     1021 caaaacgctt gcacgttcct cgccaagcac gttcgagagc tggcctcgga gcgcgatcgg
     1081 cgttacttct agacacctct gtttgcgacc accgtttaga aaaacgctcc gaccggattc
     1141 ttgccacgtc aggagatggt ggctcttttc atcagcctgt tagtataagc gcggttaaat
     1201 cttaacgggt ctttgcctga agttcggatt gaaccacccg ccctcgggtt cgatcaatcc
     1261 actcacctct ggtggccccc atgtgccggg ttcgtactcg taaacaggat caacatcgcc
     1321 cagaatcgga tcgacgattc gccatgcctg ttcgacgtaa tcttgacgcg caaaacgtgc
     1381 cgaatttccc cgcatagcat caaacaagag ttcttcgtaa gcccccatct cagcagggtc
     1441 agattcctcg ctgattacca gctcgacgtg ttgtccttcg ggctggtcgc ttgctttctt
     1501 caccaaagcg ccgaccgcaa tcatcagatc gggtgttatg cggaaacgga agtaattagc
     1561 tggcggcgca acctcggaga aaaccgccgg cggctggcgc aacttgacca gtacttctgt
     1621 cgccgtgacc gggaggcact tgccggccct gatataaaac ggcactccct tccagcgcca
     1681 cgagttgatc tcgagcctta gcgccacaaa ggtttccacc cgtgaattta cggtgacgcc
     1741 cggttcatcg cggtagccgc gaaactgacc gcagataaca tcccgcggct gaagcggacg
     1801 aatccctttc agcacttttg ccttttcatc ccgcagcatc tcggcatcca gacccggcgg
     1861 aggctccatc gcaatgttcg acagaacttg cagcagatgg ttctgcacga cgtcgcgcac
     1921 agcaccggtt tgatcataaa acttgccgcg accctcgatt ccgaaacttt ccgccatcgt
     1981 gatctgcacg ctttcaacga actgccggtt ccacaacggc tcgagaaatg aatttgcgaa
     2041 gcggaagaag agcaagtttt gaaccgtgtt cttgcccaga taatggtcga tgcggaacac
     2101 gttctcttca ctgaagtggc tgtgcaccac caggttcagc ttctgcgccg tagccaaatc
     2161 gcgcccgaag ggcttctcga gcactacacg cgcgccccgg gcgcagcccg acgagccgag
     2221 ctgatgaatg acgatgtcga acaacccggg tggaattgcg aggtaatgca gaggatgctt
     2281 ggcatccgcc aactcgcgcc gtagctgttg aaaagtcgcc gcatcgccat aatcgccgtc
     2341 gatgtaacgt aaacagcggc tcaattgggc gaaagcatcc ggattgaggc cgccgaagcg
     2401 ctcgacgcta tcccgagctc gcgccttcag ttggtcgagc gtccaaccgg ctttggcgac
     2461 gccgataacc gggatgtcga gattgtcgcg ctgggcaagc gcttgaagcg cgggaaatat
     2521 ttttttatgg gcaagatcgc cggttgcgcc gaaaaatacc agcgcatcgg cgggagctgc
     2581 gtttgacatc attttatcct tcgcggtttc aaaggtaggt tcatcggaaa tcctcttcaa
     2641 tgcctcctgt gtcgtacccg cagctcatgg tgcgccggcg aatagaagta gtagcgctca
     2701 tcaacacgac gccgtccaac gtgccgatcg tcagagcgtt cagggccgat ctgatatttc
     2761 atgtttcaag ccctacttcc ccgcggtcga gatctgtccg gcaacatcgc gcgcgggata
     2821 acgcgctggc ggttttgaca tggagcgaaa tctgcgaggg gcaggcgatt cgaacgaggc
     2881 cgggctcgag tcatgttgct tggctaaggt ccaaactagg agcgaactcg gcaagctttt
     2941 tcacttgcct cccacttgtc tgcctcccgt ggttttgcgc gtctcgacga ctctattcca
     3001 gctttgccaa tcgaagagca gaatggcatc gcctccggaa gctacgttgt actgaacgcc
     3061 tttgtaaaga aatgacctca ccggaagatc ggagattcgc ttgaactcct tgctgccggc
     3121 aacctcggtg gcgatataat aacggccgtc gctttgtttc gggtccgtaa caaacgggac
     3181 atcgacgccg ccttctctaa agctcacttt gatccaattg ggcccttcac tggtcgccac
     3241 tccgggagtc aaccgcggca acagtaagga cttcgttcca gaggcatcct tgtattgcgc
     3301 gacaacgtcg ttcgacaagt agaactggac ctgttgcaag tcttctaatc tcaggctgtg
     3361 aagctgtcga tgagaatagt tctgaaaagc aaccggcgga gccgacgagc agccaacgag
     3421 cagaataatg acaggcatca ttgtgagcca gctaaggttc tgtctcctta ccaatcgttt
     3481 agatgagccg acgcatgcgc acaccatctc ccgattccgc gaactcaaac ccgtttcttt
     3541 catttgcatc gcgatatctc ctgtaatttg ctgtagctcc gtgagtctaa atgcagagaa
     3601 attgatccat cccgaattgc gcacccggcc acagtgaaca gcagaacgtg acccgagtcc
     3661 atcgaaacag cgcctcctgg tgcgatctcg ccaatggatt catcgttgtg cgaactcgcg
     3721 ccatccttgg agcaggtcat cgactaattg ggttgccttc tgccggtagc gtttctcgag
     3781 cttgccggtc gcgccgagca gtcgttttcg gtcataggtg ctcagcagca cagttcggtc
     3841 ctgaagtcgt cccgcgccga gctggacacc ggcgtcgtag ctgattgcgg cgagatctcc
     3901 gacagagcgg aggtccgtca aacgaatctg actgtaagac ggatccaggc tgcgaatcca
     3961 ccagtcctgc aatcgttccc cgcgagccat cacctcagga accataagct ccggtccggc
     4021 cgccagtatg ttgtgcctca accgcccgag ctgtttgctt ccgtcgataa tgcggagcgt
     4081 tggattaacc atcggagtct tcaggcaact cagaccgccg agatcgccga tctttttgga
     4141 ttcgaggagc tcatcgtcag ccggatcgtc ggacggcccg cgcacgcgga tcatgatttt
     4201 cagagtcaca gcgctaccga cgccgctttg aaccgagccg gcgcgaatga tgcggaagta
     4261 gtccgtcccg agatcgcggc gctcgcgcaa catgatctgc gcaaacgctt ccatcccgcc
     4321 cgccacggct ttcatcgacg catcggccag cggttgcatt ttgctctcgc cccacgcgag
     4381 caacgctgcg cgggtcgctg gcgccgcggt ccgcagccgg cccacgatgt cgggtggagg
     4441 cggcaaatga tcaggctcta tgagaccccg cttgtatcct tcgacgaaac gatcaaacaa
     4501 cctgtcacga tcctgttgcc aggagcgccg gcgagcgacg agttcgaccg aacccaggaa
     4561 ccgcacgata tcgacaacag ccggtccgct ggcagagtca tcgaagtcgt cgagccccca
     4621 tgcatcttga gtcagcgcaa actgctcgac gtgcgcgtcg ccgtgcaaac gaataactgg
     4681 cagcccttcc agatccccgg cgaagtcgtc gcacactcgt gcgatccagg aacggttgac
     4741 gaatcggaaa taattgtaag agtcggcacg gagccgtttg atcagctcgg gtgtgccgcg
     4801 ttcaagagat ttcgggtcgg cccggagcgg cgccgcggcg tgggccgttc ccattactga
     4861 aacagcgatt acagcgcaca gacaagcgaa gtggcggagt tgttggaagc aatgcatgct
     4921 ctggtttcgt ttcttgcgcg ccggtgcgca ggctcgtatt gcaggacctt agttaactcg
     4981 cgtaggcttc cgccattggg cggttggctt ttatagcgac agcgcgcaca gcgcggtgat
     5041 cacctgccgc agatgatgaa gcgggactct ggctgaatgt tgccggcatc ctgactgctg
     5101 atcactttcc aatcgaaagc aatgcgacga taaccggtcc ccaggcggta agcacgatat
     5161 tgccgatagc atagggaacc gtgtagccca gggccggcag tttgctttgt gcctcgtcct
     5221 gaattgcgcg cagcgccgcg gtcatcgtgc cagcacccga acaagccccg agaaggataa
     5281 ccgggttcat cctcaagaca tatcgaccga ataaaatggt cacaatgtgc ggcagaacag
     5341 ccgccaccaa acctacgaaa accaggctta tgcctgattt ttgcaacccg gcaataaaac
     5401 tcggacccgc gctcaatccg acgactccaa taaagacgct caaccctacg gtgtcgaaaa
     5461 cccacatcgc agcttcggga atgcggccga acgcaggaaa tacagagcgc agccaaccga
     5521 acactaatcc catgatgagg gcgccgccgc tggcggtaag cgtaatcggc agcccggcga
     5581 tcgtgaccgt aagaaggccg acgagcccgc cgatgaaaat gcctgtgccg acaaagatca
     5641 tgtctgtcgc gttgctcgac cggtcggcat aaccgatggc ggccacggcg cgatcgatat
     5701 cgcgtttggc gcccaccagg ctcagtaagt cgccgcgttc aacttgcgtc gtcatggcga
     5761 tgggcatttc ggctcccgcc cgcagcagct tgcgcacaaa gaccccgcga aagtgcatac
     5821 gccagggctc cgacgtccag agttccttga acgttttatc gacaagatcc ttgttcgtca
     5881 cgacgacatc caaaacctca aggggaaagt ccagtagctg tttgtcggtg acttcaggcc
     5941 ccacgacact actgcgctcg accaggattt cggctcttgc cataaccgca atcacatctc
     6001 cgcgatgaat gatcgtggtg ggctcggcct cgataatagc accctcacgg cgaatacgct
     6061 gaataaaaac acgtacctct tgtggcattg cttcgatttc agcgatgctc ttgttggcga
     6121 gtttttcgtt ggtgacgcaa tagacgcgca catcgaaggc acgcgaaggt gatatgatgc
     6181 cgggttgttc ttcgctctcg cccgacacct gcgcctgcat cttcttggcc tcttcttgta
     6241 gattaactcc cattaacttc ggtccaatct tcggcaggaa ccagacgacc gctgcggttc
     6301 cgacaagata ggtaacggca taagccaccg gaatattgtt aagcagatgg gctttttctt
     6361 ctgccgaaat tgcgagacga ttgatcgcgt ctccggctgt accgatcacg gtcgactcgg
     6421 tgaaggcccc ggcgagcaaa ccggctgccg tcccgatatc gtagtcaaag agtttagcgg
     6481 ctatgagggc tgcagcgaga caagtggtgc acagaaccaa cgtgagcgtc acttgagaga
     6541 gcgcatcgcg tttgagcccg cgaaagaatt gcgggcctac tttgtagccg gtggtaaaga
     6601 gaaataaatc gaaaaagacc gtctttacca gcgcagggac tttgacatcg aactggccga
     6661 tcaatactcc agcgaaaaga gtcccgacga cagtgcctaa actgaaactg ccgagcttca
     6721 ctcggccaat tgcaaaaccg atggcgagcg cgagaaaaat ggccaattcc ggattttgct
     6781 gcagggcttt gatcaaataa tccatagttg ctatctcctg atcgttgcgt cctgcgactc
     6841 gaagtgactt gtcacagcga tcgatttcct gttcgacgtg ggcgtccccg tgtaactgcg
     6901 caaactggca agtctttcag gtctgcgccg aacacgtcgc agatgcgcgc gatccagggc
     6961 cggttgacga atcggaaata gtcgagcggg tcagctcgga gtcgctcaat cagctcgggc
     7021 gccgggcgtt cgagagattc cggctgggct cggagcgacc cctgtcctcg cgccacaccc
     7081 atcaacgcag cggagatcac cgtgaaccca tagacaaagt gccttaggcc tcattgttcg
     7141 aacttggggt tgatcaggtt ctcgaaggaa aaaatctcgt cccatctttg ctgactaaga
     7201 agctttttct ctgttacgac gatgtcgtgg agacttttgc ccgtctcgta gccttctcgc
     7261 gcaagctcgg cgcattgccg gtagccaagc gatggcttaa gcagagtcac gatgcccaga
     7321 gagttgagca ccatgtcacg cgtgcgctcg gcgttcgcgg tgatgccctc gacgcaatgc
     7381 acacgcagac tattgaccgc gcgctccatg gtcgcaatcg aggcgaaaag cgcaaaagtg
     7441 atgaccggtt ccatgacgtt gagctgcagc tgaccggcgg acgctgcaag tgtcacggtg
     7501 acgtcgagcc cgatcacgag gaagctcgtc tggttcacga cctcgggaat taccggattt
     7561 accttgccgg gcatgatcga agatcccggt tgcatttgag gcaggttgat ctcgtttaac
     7621 ccgcagcgcg gtcccgaagc cagcatgcgc agatcgttgc ttattttggt gagcttggcc
     7681 gcggtgtgct tgatcacgcc tgataacagc acgtagtcac ccgtgtcgga agtggcctcg
     7741 atcaggtctt cggcgaggca gaactggatg ccggtgatgt cggaaagatg tttcgtcgcc
     7801 agcgcgggat aaccgggcgc tgcggtgacc gtcgtgccga tcgcggtagc gcccaggttt
     7861 acacactgca gcatttgcct cacgctggcg agtcgttcga cttcttcccc tattgttgta
     7921 ccccagccgt gaaactccga gccgagggac atggggaccg cgtcctgtag gtgggtgcgg
     7981 cccatcttga gcaccttgtc gaattcccga gccttggcga agaaggccgc ctgaagttgt
     8041 cgcagcgcct ccatgtagct ttccaagcgg agaatcagcc cgaggtggaa ggcagtcgga
     8101 tagacgtcgt tcgtcgattg tccgaagttt acatggtcgt tcggattgac gtattgatac
     8161 tcacccttct catgacccaa aatttcgaga gcgagattag cgatcacctc gttggcgttc
     8221 atgttggttg aggtgccggc gccgccctga atgaagtcgg tcacgaactg gtcgagcatc
     8281 tcgccggcga tcagccggtc gcacgcgcga atgatggcgt cggcgatctt tgcgtccagc
     8341 acgccgagat cccggttcgc catggctgcg gctttcttga cgtagccaaa cgctttcacg
     8401 aagtggggct cgcgtgatat cggcattccg gtgatgtgaa aattctcctt gccccggagc
     8461 gtctggacgc cgtagtacgc atccgccggc acctcgcggt caccgaggaa atcgtgctct
     8521 attcgaactt gcgtagatac catcggcgcc tctctttttc tgttatgttc tttcacgaca
     8581 atgctcgttc caattttctg gcgagatcgc ggttcacgag accttgcagc gttacgcgca
     8641 agtccggccg cgcgtccatg aaccgccgca ggcttggaag cgaccaacgc atgtaacgcg
     8701 cgggctcggt gaaggtcact tccaccggcg atggttcgcc cgtgagcgcc agcgccgtgc
     8761 cgatgatgtg gcccggtgtc agcgccccga tgcgctcacc atgcttgtga acatctgcgc
     8821 tcccggtgat cgggatgcag agggacgaca ccggctcccc ttcggtcaag accctctccc
     8881 cgggctcgcc attcttccat tcgcccacta acgacagtga aacgaactcg cggggacgaa
     8941 gcgagcggaa tcccagatca tagagctttt gctcgtcttg ggagagcact accggacggc
     9001 gctcgaggta gaggcgcgcg atctgataga gattgatggc ggtaaatacc agtgcccaga
     9061 gaaccggcgg ccagagcacg gtaccctgga ggaggaagta cgggatattg gtgagcgcgg
     9121 cggcaacggc aaaccagcgc agccacagga tgtcgcgcac cgaatacgcc gccagcatca
     9181 gaatattcga aaaatgtacc agatagtcta tagcgtgcat attgatctag tctcctttct
     9241 ctaattgctg aaaaagaatc gcgatccggt tttcccttcc aacaagttat cctggatcat
     9301 gcgcagccac agcttctgcg cgattccgtc aaggggcacg gtctgctccg accttaggag
     9361 ttttgtcgcc acggtgactt ccccagccga ctcccagagg atctttcccg tctgcgtgtc
     9421 ccagagctgc agccatagcc tcatggtagt gactcgactc tggataaagt tccagccaga
     9481 gatcgaaagc cggtcgatga tcacttgatt gaactccgcg agcccaggca gcagcacata
     9541 gcgggaggaa agtgccgatc cgatccgctc taacggcttc cgctccagga tgccgccgcg
     9601 accgaagccc gccatcagag tgccgtactc tccggccagc ccttgttcgt tgatcgtgtt
     9661 gactgtctca tgggccgcaa ttccccggat aggcggggac gcttccgaga gcgccgcgtt
     9721 gagggcgtga gacagaggcg ggttcaagcc ctggagaccg gccggagcga gaaggccgag
     9781 tgtcgccacc ggctcgcgct ccagtgcggc gacatcgaga agctctgatc ttggcgttga
     9841 cgtggtgtgt gtatccgaca agtataatgg tgtgcaacca gccaagagag ctgccgcaaa
     9901 catcaggatt gttccgtgaa atctattggc gaggaaaact tgaatcatga tggactcctc
     9961 tatatttcgg tcaaccgcac cgaaggatcg tctcgacatg gctggaacct tgcggttgtc
    10021 gaggaggtcg cgttcgatcc ggcttcctca ccgtatcgcc agcatccagg gcacgccgat
    10081 gacgagaaat acggcaaaaa agatcacgcc gaaaatcgcg cccaggcgcc agtaatccgc
    10141 cgaaggcaga taaccgctgc cgtaatacac cgggcttggc ccgctcgcgt acggcgtgat
    10201 gatgcccatg attccgagct gcaagcacat gcccagcgcg agcgtatgca tgttgactcc
    10261 gggaatcgtc gacgcgaccg ccagcagcac cgggatcatg gctgtgacgt gggcagtaac
    10321 gctcgcaaac aggtagtgac cgaagaagtt aataaataat agagccgcga ctgctatggc
    10381 tggggtgacg ccggtgagtt gaccggcgac ggtctccgca aaccatttca caaaacccac
    10441 gcgagtgagg ccgtcagcga gcgcgatcaa agtcgcgaac cacgccaacg tgttccaagc
    10501 cgctgaattt ttgacgatgt cgtcccaggt cacgacgttg aggatcagca tcaatgaaat
    10561 gacgatcagc gcagcggtgg tcgcgttcac ttcatctccc cgaaagatcc acatgaccag
    10621 agcgaggagg accagaacgg ccagtgtaat ctcccgttta ctgagcgcgc ccattttgtt
    10681 cagctccgtt gccgcccagg actgaac
//
LOCUS       DASUVS010000059        13861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_676852, whole genome shotgun sequence.
ACCESSION   DASUVS010000059 DASUVS010000000
VERSION     DASUVS010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13861
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676852"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..588)
                     /gene="selD"
                     /locus_tag="VF182_03385"
                     /pseudo
     CDS             complement(1..588)
                     /gene="selD"
                     /locus_tag="VF182_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
     gene            complement(694..2625)
                     /gene="selB"
                     /locus_tag="VF182_03390"
     CDS             complement(694..2625)
                     /gene="selB"
                     /locus_tag="VF182_03390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEX6436147.1"
                     /translation="MPYIIGTAGHIDHGKTSLIKALTGEDTDRLKEEKERGISIDLGF
                     AQLDLGDGNLAGVVDVPGHERFIKNMLAGAHGIDLVLFTVAADDGVMPQTEEHLDIVH
                     LLGIKIAIFVITKADLVSRARIAEVEEEINILTLGTVLEDSPVIAVSSTTGQGLGELK
                     QLISQSLKSIDKPAPGGYFRLPVDRAFVLQGHGVVVTGTARSGEIRTGDRVRCLPGEQ
                     IFRVRSLQVHGQSVDSAGWGQRVALNLTGPDTAEVARGHVICHEKISLTSNRFDAYLE
                     VRPAARKGIKNHQNVRVHLGTAERLGKVIILGERNHIEPKQSAYCQITLAEPILALRG
                     DHFILRDETAQRTLAGGMVINPWARRHKRSDGLENKLEALYSGDITQLTEAFLEESEA
                     FALPLESIYQFLDCREELIRGSIEKMKNVRAVSAEGEKVYTTQAKWQRLKEQISTILK
                     DFHGTHPLVPGMDMEELRGKLSYELSPKVFRLVVDGLIDERLIAKEENLLRLAQHKLQ
                     LGGQDKLLMERIKKILGQQELAPPELKQIEKDVAVPRAKLAEVLRLLERERSVIKVAT
                     DLYFMSDCVDKVKNLLCKHLAEKGEISAATFRDLLGSSRKYTIALLEYFDREGVTLRI
                     GDVRRLKSSSPTEKRGSAH"
     gene            complement(2854..4242)
                     /gene="selA"
                     /locus_tag="VF182_03395"
     CDS             complement(2854..4242)
                     /gene="selA"
                     /locus_tag="VF182_03395"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX6436148.1"
                     /translation="MTEQATNLLRELPSVDRLLKHPRCEALLARYNRDYLIQKCRDAL
                     DLARSEIRQGKSIAGAVVKDEAIAARVEAQIAAESQPGLVRVVNATGTILHTNLGRAL
                     LSQAAIDAVVTVAQSPVNLEYDLTAGKRGRREHTVEKLLIELTGAEAATAVNNNAAAV
                     LLALNSLAEGREVIVSRGELIEIGGAFRIPEIMAKSNAILKEVGSTNRTHPEDYQRAI
                     NEKTALLLKVHTSNYKVVGFTAEVDLAQLVAIGKKHNIPVMEDLGSGAFIDLSRYGLP
                     KEPIVGERIDAGADVVTFSGDKILGGPQAGLLVGKKSLIDQMNKNHLQRALRCGKLTL
                     AALEGTLRRYRQSPDITREIPTLRSFTRSLDEIRESGQELLPRLQSALGGDYRLTLEE
                     STSQIGSGALPTEELPTLVIAIVHETLNANRIAESFRRADPPIIGRIQDDRFLLDLRT
                     IFDAEDLLPNFD"
     gene            4594..5337
                     /locus_tag="VF182_03400"
     CDS             4594..5337
                     /locus_tag="VF182_03400"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX6436149.1"
                     /translation="MHFAFRFRQGMKAAIPIWIAFVPSSVAWGIAAQAHGMRFAEIVV
                     MSAWVYSGPAQFAVLAPLSEGKSSLQVLVAGFLMNLRFLPMSAALAPYFHGVNRFSLL
                     LSSHVVSASSFIVPYLQFQKERQDAGDLELTEPTDGYGNLAFFLGVGASSFLVWVIGS
                     AFGYAVALGFPAGFDEALKFILPGYFAGLLVVEMRGWGMPVVCLAALFSAVPGALVSP
                     GWGWLATATLVAILVWGAEQWKYRASALS"
     gene            5307..5627
                     /locus_tag="VF182_03405"
     CDS             5307..5627
                     /locus_tag="VF182_03405"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX6436150.1"
                     /translation="MEISRIGIILIMGFMAFAVRALPQIILVGRKFPDSLERLLRYLS
                     YAFICSIIGVTLFMTAGKFELHLAPSRALALALAITVAYITRSAVSGMLAGMLLVLIL
                     SWLR"
     gene            complement(5697..6815)
                     /locus_tag="VF182_03410"
     CDS             complement(5697..6815)
                     /locus_tag="VF182_03410"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6436151.1"
                     /translation="MPSMMVATLGIALPEIRQSFSLSEIEGGSLFSVLMIVAAITSSV
                     AGRFADRYGRKTILISGLTLLAVGFGFAGITSSRLLFFLSLGITGLGYGFTPPALYAV
                     MSDLLPNKRGLGASLVSVSYGIGGAIGAVLASRIIAVSDWRAAFVTVGVIGAVIMLLQ
                     LYWIRNIRSIKSAVRGGSFRGSLSLPLLILALAEFIGGSVFWSSAAWSPTLLRSDKAL
                     TLKETGWVMGVLSLANMLGSFCLGALSDKLGRRRVIVLSSFPAALAAFVVFYWLQSLL
                     GLVLGMLVFGVLKASVPALVVALAQEASPPASAGTASGIIMALHYTAGVIAPLIAAKL
                     ISATADIVWAMILCTSVPLVIYGTLIATVRGRSNSQAA"
     gene            complement(6900..7238)
                     /locus_tag="VF182_03415"
     CDS             complement(6900..7238)
                     /locus_tag="VF182_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436152.1"
                     /translation="MRIYTGADNESHFEEKEIDLKFNGDMEVSELEPATGVFFRQAPA
                     NHLNNFHPAPRRQYIITLSGEVEIEIGDGTIKRFGKGDIMLADDSTGRGHITRVVGNQ
                     PRVYVTIPLK"
     gene            7377..8285
                     /locus_tag="VF182_03420"
     CDS             7377..8285
                     /locus_tag="VF182_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX6436153.1"
                     /translation="MPELPDITVYIEALQDRLLNQPLQKIRIASPFILRSFDPPIGAA
                     EGKKVVGFDRIGKRIVFELEDDLFMVFHLMVAGRFHWKKRGAPVPRKFGHAALDFPPG
                     TLLLTEMGTKKRASLHLVRGREHLAEHDPGGLEIFGSDLNAFRAALTDENHTLKRTMT
                     DPHVFSGIGNAYSDEILHRAGLSPIKQTQHLSEVEIERLYRATKEVLREWIKRLREDT
                     GKNFPERVTAFRDEMAVHGKYRKPCPVCGSPVQRIAYAENETNYCAQCQTGGRLLADR
                     SLSRLLKADWPKTLEEMEEGRKARTE"
     gene            complement(8291..9022)
                     /locus_tag="VF182_03425"
     CDS             complement(8291..9022)
                     /locus_tag="VF182_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6436154.1"
                     /translation="MGVTNEQSGTNVHEIADRIYRINTPVVIEGGPGGFSFNQYLIVD
                     DRPLLFHTGPRKMFPLVREAVASVIPVEQLRYIAFSHVEADECGSLNQWLAAAPQSLP
                     LCGSVAALVSIEDLADRSPRGMADGELLSLGEHSVRWFDTPHLPHAWECGFLMEEATK
                     TLLCGDLFTQGGADHPPITKSDILGPSEAFRGAMDYYSHTKNARPMLERLAATQPTTL
                     ACMHGSAWQGDGAALLRALADSLSP"
     gene            complement(9128..10264)
                     /locus_tag="VF182_03430"
     CDS             complement(9128..10264)
                     /locus_tag="VF182_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436155.1"
                     /translation="MTTNGKPPVGISKNIKQIGRMDLPGGGSIVVENGYAYVGHMDPP
                     HGTSIIDARDPKNLKVIAHLEIPEGLHSHKVRVSGDLMLINYERYKARQELDSGLKIF
                     DISNRSKPREIAFFKTHGKGVHRFTFDGRYAYISPTMEGYQGNIAMVLDLKNPEKPEE
                     VCRWWMPGQWTAGGETPTWRGTDTRCHHPIRRDNRLYISYWHGGFAIVDISDMSKPKT
                     ISSLDWSPPYPCPTHTTLPMLNKIMERDWLIVTDEEVGEKLNETHNAFLWVVDITKEE
                     LPLPVATFIVPFEGKSTHLNRFGAHQPAEQVYGNTLYVTWFAGGLRAVDFSNPYIPKE
                     LGYYVPMPGKGQSVVMSNDVFHDKDGKLYLMDRYEGLEILEPQI"
     gene            complement(10462..11103)
                     /locus_tag="VF182_03435"
     CDS             complement(10462..11103)
                     /locus_tag="VF182_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEX6436156.1"
                     /translation="MNVNYFEPKKTGLLFFDILNGYVAAPEPGKQRVLKPWIQNAVRL
                     SKAGRAAGLPVFFAKGNHRPDNATTALLLTDTNNSLTPWPNGEVSKPKMHVIAGDKSS
                     DPLAELDPSPDDYYIVKYRWSSFYQTYLDLALRARGIDTLIISGGSTDVGVASTLFSG
                     RDMDYNMIVASDACGTSHDQRAHDMLMELIFPRMARVRTTEQIIQMIEESRSK"
     gene            complement(11113..11874)
                     /locus_tag="VF182_03440"
     CDS             complement(11113..11874)
                     /locus_tag="VF182_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX6436157.1"
                     /translation="MSLENIRMVMVRPRGSGNIGSVARAMKNTGVPELAIVGKARTKG
                     FWARAMAVHGRDILNNAIFYETLREAVADCRLVVGTTCRAGLYRAHSRSPREVAGEIV
                     AAAATGKAALILGPEDHGLSNEDLKHCQLLINIPADPEYQSLNVAQAAMICLYEIYLA
                     SLGEPMKESLPLARAEDVERLYDRMRTSLLKIGFLDSQNPEHMLFAFRRILGRTGLED
                     RDVRILTGLFRQIEWYAEHGWKVVQEKEKQGIKIR"
     gene            complement(12115..12765)
                     /locus_tag="VF182_03445"
     CDS             complement(12115..12765)
                     /locus_tag="VF182_03445"
                     /inference="COORDINATES: protein motif:HMM:NF025068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HEX6436158.1"
                     /translation="MITRRPIICCILSALLLVSCSAISSPAPERVIRVKVVGDVSLRA
                     RNSRWPEEVRGLVEAASDYYESEFGIRLVSQSVSAWPLEERIPGTPELLARLEQDFPQ
                     TKNHDYDLVIAFTAEGISRYTRAGRPRVDRIGNCQQGLGGYAVVPVSRVVHYTGSQSN
                     PQPEVIALIHEIGHIFGAQHVADAASIMHEDFDYRTEFDAANRAVIERNKLCRFRK"
     gene            complement(12728..>13861)
                     /locus_tag="VF182_03450"
     CDS             complement(12728..>13861)
                     /locus_tag="VF182_03450"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX6436159.1"
                     /translation="IRLAATTLSTVGSTAGTLYAVSTCGSIFGTLLTAFYLIPAAGVR
                     NIIHGLGITLVCLSLFVVPLVRLRGITLARAVTAVTVILGSFMMPWTPGALARIKTLV
                     EKETFYHRIRVEEDDEARYMYFDRTLQSAMNLKDPTALRLIYSRYTSLGVAFRPDAKK
                     MALIGLGGGSIPKKLLKEFPSLEIDAVELDPEVIQIAKTHFNVRESNNLRLHAQDGRM
                     FLSRTPHQYDIIMLDAYFTDAMPFHLATKEFFELAQKKLTPNGIIVANLISAVTGPSG
                     KIARSFVKTQGQIFPQIYVFAARRPDNVSVDTIQNVIVIATRDKQRLDIKEIVKRATS
                     IDKGLFPNPIQDIGIAYFDRPLPDQDVPVLTDDYAPTDNLLHP"
BASE COUNT         2929 a         3779 c         3919 g         3234 t
ORIGIN      
        1 gcgcatgata ctggatgccg tggcgttaag cgtagtcatc gatcgcacgg cttggtcgac
       61 gctttcttcg gcagccttac cttttttgag ggccgtggtg atgatgccgg tgccgagagg
      121 tttggtcagg accaacgcgt ccccttcctg cactcctacg ttgcggtaga ttttatcggg
      181 atgaatggtc ccggttacgg ccagaccgta cttgatttcc tcgtcgatga tgctgtgacc
      241 gccgatgatc actgcgccac tttctttgac cttatcggcg ccgcctttga gaatctctcc
      301 caggatttcg atctccatct tccctttcgg aaaacagacc aggttcaggc atgtcttggg
      361 ctcgccgccc atggcataga cgtcgctgag tgaattggcc gccgagatct gcccgaaggt
      421 atacgggtca tcgacaatcg gcgtgaagaa gtcgactgtg ttgacaatgg caagatccgg
      481 tcgcaggcga aatactccgg cgtcgtcgga agtctccgtg ccgactaaca tgtcagggtg
      541 gttgaacttt ggcagatcgt gcaaaacttg cacgaggtcg gctgggccga gcttagcggc
      601 tcaacccgcg gctttgaccg actgcgttaa tcgaaccgct tttttctttc ccatcatcat
      661 agatcgtgag tgtagttcga tcgaactttc aattcaatga gctgagccgc gcttttccgt
      721 tggcgaagag gatttcaggc gccggacatc gccgatcctc agtgtcacgc cttctcgatc
      781 gaaatattcc agtaacgcaa tcgtatactt ccgacttgat cccagcaggt cacggaatgt
      841 ggcggcggat atttcaccct tttcggcgag atgcttgcaa agcagattct taaccttgtc
      901 gacgcaatca gacatgaaat acagatcggt ggccactttg atcacagacc gctctctttc
      961 gagcagccgc agaacctcgg ccagtttcgc gcgcggcacc gccacgtctt tttcgatctg
     1021 tttcagctcc ggcggcgcga gttcctgttg accaaggatt ttcttgattc gctccatcag
     1081 caacttgtct tgtccgccaa gctgaagttt atgctgcgcc agccgtagca ggttctcttc
     1141 tttagcgatg agccgttcat cgatcaatcc gtcgaccact agccggaaga ccttggggga
     1201 gagctcgtag gagagcttgc cgcgcagctc ttccatatcc atgcccggaa ccagcggatg
     1261 ggttccgtgg aaatccttga ggattgtgga gatttgctct ttgagacgct gccatttcgc
     1321 ctgcgtcgtg tagacttttt cgccttcggc gctaacggcg cgaacgttct tcattttctc
     1381 gatagagccg cggatcagtt cctcgcgaca atcgaggaac tggtaaatgg attccagcgg
     1441 caaggcaaat gcttcgcttt cctccaggaa cgcttcggtt agttgggtga tgtcgccgct
     1501 gtataacgct tcaagtttgt tctccagtcc gtcggatcgt ttatgtcttc tcgcccacgg
     1561 attgattacc atgccgccgg cgagtgtccg ctgagcggtt tcatcgcgga gaatgaagtg
     1621 atcgccgcgc aaagccagga tcggctcggc caatgtaatc tggcagtaag ccgattgttt
     1681 aggctcgata tggttccttt cgcccaggat gatgactttg ccaagtcgtt ccgcggtgcc
     1741 caaatgcacc ctcacgttct gatggttctt gatgcccttg cgtgcggccg gccggacttc
     1801 cagataggcg tcgaagcgat tcgacgtcag cgagattttc tcgtgacaga taacgtgacc
     1861 gcgcgcgact tcggcggtat cgggtccggt gagattcagc gcgactcgtt ggccccagcc
     1921 ggcgctgtca acagattgtc cgtgcacctg aaggctgcgc acgcgaaaaa tctgctctcc
     1981 gggcaggcag cgcacccgat cgccggttct gatctcgccg ctgcgtgcgg tgccggtcac
     2041 caccacgcca tgaccttgca gtacgaaagc gcggtccacc ggaaggcgga agtagccgcc
     2101 tggcgccggt ttgtcgatag acttcaatga ttgagaaata agctgcttga gctcaccgag
     2161 accctggccg gtggttgagg aaaccgcgat gaccggcgaa tcttccaaaa ccgttccgag
     2221 agttaagata ttgatctcct cttcgacttc ggcaatgcgc gcccgcgaaa ccaggtccgc
     2281 tttggtgatc acgaagatgg caatcttgat cccgagcaaa tgaacgatgt ccaggtgttc
     2341 ttcggtctgc ggcatgacgc cgtcatcggc ggcaacggta aagagtacca gatcgatgcc
     2401 gtgcgcgccc gccagcatat tcttgatgaa gcgctcgtgc ccgggaacgt cgaccactcc
     2461 agcgagattg ccgtcgccga gatcgagttg ggcaaagccc aagtcgatcg aaatgccgcg
     2521 ctccttttct tccttgagcc ggtcggtatc ctcgccggtc aatgctttga tcaggctggt
     2581 tttaccgtgg tcgatatgtc cggcggtgcc gatgatgtaa ggcatctttc taaacttttt
     2641 ctcaggctat tgaaaaaggc tcatctgctt cgttgcgctc gatcgatttc gcatcaacgt
     2701 acatgagtac gcttccgctc atcgctcttc gcgcgcctcg catctgaacc tttttgaaca
     2761 gcctgaaagg catttcgttg ctgcttctat aaaatcggct ggatattgtc attcaaattt
     2821 gtgtttatct cttagcgtct ttgcgccagc tgttcagtca aagtttggaa gcaagtcctc
     2881 ggcatcgaag atcgttcgca gatccagcag aaaacgatcg tcttgaatcc tgccgataat
     2941 cggcgggtcg gcgcggcgaa agctttcggc gattcgattg gcgttcagcg tctcatggac
     3001 aatcgcgatc actagagtgg gaagctcttc ggtgggaagc gcgccgctgc cgatctgcga
     3061 agtcgattct tccaaagtta agcggtaatc gccgcccagc gcggactgta atctcggcaa
     3121 cagctcctgg cccgattcgc gaatttcatc gagactgcgg gtaaagcttc tcaaggtcgg
     3181 gatctcccgg gtgatatccg gcgattgacg gtaccggcgc aaagttcctt ccaaggcggc
     3241 caacgtcaat ttgccgcagc gcagcgcgcg ctgcaggtgg tttttgttca tctgatcgat
     3301 aagacttttt ttgccgacca acagccctgc ctgcgggccg ccgagaatct tatcgccgct
     3361 gaaagtgacg acatcggcgc cggcatcgat gcgctcgcca acgatcggct ctttcggcaa
     3421 tccgtaacgg ctgagatcga tgaacgcgcc gctgccaaga tcttccatca ccggaatatt
     3481 gtgttttttg ccgatggcca cgagctgcgc caaatcgact tccgcggtaa atccgacgac
     3541 cttatagttg ctggtatgca ctttcaggag cagcgccgtt ttctcgttga tggctctttg
     3601 gtagtcttcc gggtgtgtgc gattggtgct gccgacctct tttaagatcg cgttgctctt
     3661 ggccatgatc tcgggaatcc gaaacgcgcc gccgatttcg atcagctcgc cgcgtgaaac
     3721 aatgacttcg cggccttcgg ccaagctgtt caaggcgagc agcaccgcag cggcattatt
     3781 attgaccgcc gtggcagctt cggctccagt cagctcgatc agcagctttt caaccgtgtg
     3841 ttcgcgccgg ccgcgctttc ccgcggtaag atcgtattcc aaattcacag gagactgggc
     3901 gacggtgaca accgcgtcta tggcagcctg ggacaagagc gcccgtccga gattggtatg
     3961 cagaatcgtg cccgtggcat tcacgactcg gacgagaccc ggttggctct ccgccgcgat
     4021 ttgcgcttcc actcgcgcag caattgcttc gtctttcacg accgctccgg caatgctttt
     4081 gccttgcctg atttcacttc gcgctagatc gagggcatcg cggcatttct gaatgaggta
     4141 gtcgcggttg tatcgggcca gcagcgcttc gcaacgggga tgcttcagga gccggtccac
     4201 cgagggcagc tcgcggagca ggttcgtcgc ttgttcggtc atgaggggat taaaattcgc
     4261 ataagcgata atcgaattca acatgactgg caggccgctt gaaaaagacc caatgcttcg
     4321 ttgcgctcaa attgcctcgg gcaggttcgt gttcgtgata cgtgttcgta ttcgttcgaa
     4381 attctcgatc acgtttgcac ggactcgagc acgaggatcc gcctcgcgtt tggatctctt
     4441 tgagcagccg gcaaccagag tttttttagc aaccgctagc ggcgcggcac aataaaggat
     4501 tcgcagcggc aaaagatttg aatgagagtt tgggtagcga tcgattgtcg gtctttggtt
     4561 gatctttgaa ccgggctcag ctaagcggtt tcaatgcact tcgcgttccg ttttcgtcag
     4621 ggaatgaaag cggcgattcc gatctggatc gccttcgtgc cgtcgtctgt cgcctggggc
     4681 attgccgcgc aagcgcacgg aatgagattc gcggaaatcg ttgtgatgtc cgcttgggtc
     4741 tactccggtc cggcacaatt cgccgttttg gcgccgctgt cggaaggcaa gtcatcgctg
     4801 caagtgctgg tggcgggctt tctcatgaat ctccgttttc tgccgatgag cgcggccctg
     4861 gcgccatact ttcatggcgt caaccgcttc agtttgctgc tctcttccca tgtcgttagc
     4921 gccagtagtt tcatcgtgcc ttatctgcag tttcaaaagg aacgacagga tgccggtgat
     4981 ttggaattaa ctgagcccac agacggctat ggaaatttgg ccttctttct aggcgtgggc
     5041 gcgagcagct ttttggtttg ggttatcggc agcgcattcg gatatgccgt cgcgctcggc
     5101 ttcccggcgg gatttgacga agccctgaag tttatcctgc caggttattt cgcagggctg
     5161 ttggtggtgg aaatgcgcgg ctggggcatg ccggtcgtct gcctggctgc gctgttttca
     5221 gcggtgcccg gcgctctggt gagtcccggc tggggttggt tggccacggc aacccttgtg
     5281 gcaattttgg tttggggggc agagcagtgg aaatatcgcg catcggcatt atcttgatca
     5341 tgggatttat ggccttcgcc gtgcgcgccc tgccgcagat aattttggtc ggccggaagt
     5401 ttcccgattc cttggagcgg ctgctgcgct atctatccta cgcttttatt tgcagcatca
     5461 tcggagtgac gctctttatg acggctggaa agttcgaatt gcatcttgct ccatcgcgag
     5521 ccctggcatt ggcgctggcg attaccgttg cgtatatcac cagaagcgcc gtcagcggca
     5581 tgcttgctgg tatgttgctg gtgctgattc tctcgtggct tcgctgatgg acctgcggat
     5641 tttggagaag aatcgtttcg agagataccc cgccttggag atcgagcaat tcccaattac
     5701 gccgcctggc tattgctgcg tccccgcacg gttgcgatga gagtgccata aattactagg
     5761 ggaactgaag tgcaaaggat catcgcccag acaatatccg cggtagcgga aatcaatttt
     5821 gccgcaatta acggcgctat cactcccgcg gtgtagtgga gcgccatgat gatgccgctg
     5881 gcagtcccgg cgctggcggg gggcgaagct tcctgcgcca gggcaaccac caaggcaggc
     5941 acggaagcct tgagcacacc gaagacaagc atacccagga ctagtccgag aagcgattgc
     6001 aaccaataga aaaccacaaa cgccgccagc gccgccggaa aagaactcag cacgattacc
     6061 cgccgccggc ccaacttgtc cgataaagcg cccaaacaga acgagcccag catgtttgcc
     6121 aggctcagca cgcccatgac ccagccggtt tctttgagcg tgagtgcttt gtcgctgcgc
     6181 aacaaggtgg ggctccaggc ggccgagctc cagaaaacgg aaccgccaat gaactcggcc
     6241 aaggcaagaa tcaaaagcgg cagcgagaga gagcctctga aagatccgcc gcggacggca
     6301 gacttgatcg agcggatgtt tcggatccag tagagctgca gcaacatgat caccgcgcca
     6361 atgactccga cggtgacaaa ggcggcgcgc cagtccgaga cggcgatgat ccggctcgcc
     6421 aagacagcac cgatggcccc gccgatgcca taagagaccg aaaccaggct agcgccgaga
     6481 ccgcgtttgt ttggcagcag atccgacatg acggcgtaaa gcgccggcgg tgtgaagcca
     6541 taacccaatc cggtgattcc gagagacaaa aaaaacaaga gacgactgga tgtaatgccg
     6601 gcaaagccaa aacccaccgc caaaagggtc aagccggaga tcagtatggt cttgcgaccg
     6661 tagcggtcgg caaaccgacc ggcgacactg ctggtgatgg cggcgacgat catcagaacg
     6721 gagaacaggc tgcctccttc gatttcggaa agtgagaacg attgcctgat ttcaggcaag
     6781 gcgatcccca aggttgccac catcatcgat ggcataaagt tggagcaggg gacgatgcag
     6841 agcgtcaagc ggccacgctg tggcgcttca tctggaatga gattgctgat gggatacctt
     6901 tactttagag gaatggtcac gtagacgcgc ggctggttgc cgaccactcg ggtgatatgg
     6961 ccgcggccgg tgctgtcgtc ggcaagcatg atatcgcctt tgccgaagcg tttgatcgtg
     7021 ccgtcgccga tctcgatctc cacctcgcct gacaaagtga tgatgtactg gcgccgcggc
     7081 gccggatgga agttgttcag gtgattggcc ggagcttggc gaaaaaaaac tcctgtggca
     7141 ggctccagct cggagacttc catatcgcca ttgaacttca gatcgatttc tttttcttcg
     7201 aagtgcgatt cgttgtctgc gccggtgtaa attctcataa tcttcatgag tttggtatgt
     7261 aggagaaacc atgggcaaaa gcaaggcgca gttcgatcag aatttgcaga caaggtcaga
     7321 gtttgacggt ccgcttgtgg actaattgcc gcgcgtggta gtcagaaatt ctaaccatgc
     7381 ccgaacttcc cgacatcacc gtttacatcg aagcccttca agatcgattg ctgaaccagc
     7441 cgctgcagaa aatacgcata gcgagcccat tcatattgcg ctcgttcgat ccgccgatcg
     7501 gggccgcgga aggaaaaaag gtggttgggt ttgaccgtat cggcaagcgc atcgtgttcg
     7561 aattggaaga cgatctcttt atggtctttc accttatggt cgccggccgt ttccattgga
     7621 aaaagcgcgg cgcgccggtg ccgcggaaat tcggccacgc cgcgctggat ttccctcccg
     7681 gtactttact attgacggag atgggcacaa aaaaaagagc gtctcttcat ttggtccgcg
     7741 gcagagaaca cttggccgaa cacgatcctg gaggattgga aatcttcggc agcgatctga
     7801 atgcgttccg cgccgcgctc accgacgaga atcacacgct caagagaacc atgaccgatc
     7861 ctcacgtttt cagcggcatc ggcaacgcct actccgacga aattctccat cgcgccggat
     7921 tatcgccgat caagcagacc caacatctca gcgaggtcga gattgaaaga ctctatcggg
     7981 caaccaaaga agttctacgc gagtggatca agcgattgcg cgaagataca ggcaagaatt
     8041 ttcccgaacg ggttaccgcc tttcgtgatg agatggcggt tcacggcaag tatcgcaagc
     8101 catgcccggt ttgcggttca ccggtgcagc gcatcgctta tgccgaaaac gagaccaact
     8161 actgcgcgca gtgccaaacc ggcgggcgct tgctcgccga tcgttcgctg tcgcgcttgc
     8221 tcaaagccga ctggcccaaa acgttggagg agatggaaga gggaagaaaa gcacgtactg
     8281 agtagctgga ctaaggcgag agtgagtcag ccaaagcgcg caggagtgcg gcgccgtcgc
     8341 cctgccatgc gctgccgtgc atacatgcga gtgtcgtcgg ttgagtcgcg gcaagtctct
     8401 cgagcatcgg ccgcgcattt ttggtgtgcg agtaataatc catcgcgccg cggaaagcct
     8461 cgcttgggcc cagaatgtcc gacttggtga tcggcggatg atcggcgccg ccttgggtga
     8521 agagatcacc gcagagcagc gttttcgtcg cttcttccat caggaagccg cattcccacg
     8581 catgcggcag gtgcggcgta tcgaaccatc tgacggaatg ttcgccgagc gagagcagct
     8641 cgccgtcagc catcccgcgc ggcgagcgat cagcaaggtc ttcaatcgat accagcgccg
     8701 cgacgctgcc gcagagcggc aacgactggg gcgctgccgc aagccattga ttgagcgagc
     8761 cgcattcatc ggcttcgaca tgtgagaaag caatgtaacg cagctgctcc accggtatga
     8821 cgctagcgac cgcctctcga accaagggga acatttttcg cgggcccgtg tgaaagagca
     8881 ggggccgatc gtcaacgatg agatactgat tgaacgagaa gcctcccggt ccgccttcga
     8941 tgacaacggg tgtgttgatg cgataaatcc gatctgcgat ctcatgaacg ttggtgccgg
     9001 actgttcatt ggtaacgccc atttgctagt cctccaattt agtagacggg agctccttcg
     9061 gagccgatcc ctcgacgagt tcagacgatt tgaacggctt gagcgttttg aacgctcgct
     9121 gcgcgaacta gatctgcggc tcgagtatct ccagtccttc atagcggtcc ataagataga
     9181 gcttgccgtc tttgtcgtga aagacatcgt tgctcatgac cactgattga cccttgccgg
     9241 gcatcggcac gtagtagccg agttctttcg ggatatacgg attggagaaa tccaccgcgc
     9301 gaagacctcc ggcgaaccag gttacgtaca atgtattgcc atagacctgc tcggccggtt
     9361 ggtgcgcgcc gaatcggttc agatgggtcg atttaccttc gaaaggcacg ataaatgtcg
     9421 ccaccggcag cggaagttct tctttggtga tatcgaccac ccagagaaat gcgttatggg
     9481 tttcgttgag tttctcgccg acttcctcgt cggtgacgat caaccagtcg cgctccatga
     9541 ttttgttcag catgggcaaa gtcgtgtggg tggggcaagg ataagggggg ctccaatcca
     9601 ggctgctgat cgtcttcggt ttgctcatat ccgagatatc gactatcgcg aagccgccgt
     9661 gccaatagct gatgtaaagc cggttgtccc tgcggattgg gtgatgacag cgcgtgtcgg
     9721 tgccgcgcca ggtcggcgtt tcaccgccgg cagtccactg acccggcatc caccagcggc
     9781 agacttcttc gggcttttca gggtttttca gatccagaac catggcgatg ttgccttgat
     9841 aaccttccat ggttggcgag atgtaagcgt agcggccatc gaacgtaaag cggtgcacgc
     9901 ctttgccgtg ggttttgaag aacgcaattt cacgcggctt ggacctattc gagatatcga
     9961 aaatcttcaa gccggagtcg agctcctgcc tagctttata acgttcatag ttgatgagca
    10021 tgagatcgcc gctcacgcgg actttatgag agtggagtcc ctcaggaatt tcgagatgcg
    10081 ctatgacctt caaattcttc ggatcgcgcg catcgatgat cgacgtgcca tgcggcgggt
    10141 ccatgtgacc gacataggcg taaccgtttt caacgactat ggaacctcca cccggaagat
    10201 ccatgcggcc gatctgtttg atgttcttgc tgatgcccac cggcggtttt ccattggtcg
    10261 tcatccgttt gtctcctttt gtcaggccgt tccaaaaacg ctcaccgtat cagaaaggat
    10321 gaatgatgaa agctgaaagc agaaatttcc gttatcatcg ttccgctttc gtccctgtct
    10381 ttgcgcgcgc ctcgcaacca aacctttttg aacggcctga acaagctttt aataatttag
    10441 atcatttgct tcgagctaat gctatttgct tcgtgactct tcaatcatct gaataatctg
    10501 ctcggtggtg cgcacacgtg ccattcgcgg aaaaatcagt tccatgagca tgtcgtgggc
    10561 gcgctggtca tgcgatgtgc cgcacgcatc actggcgacg atcatgttat agtccatgtc
    10621 ccttcccgaa aacaaagtcg aagcgacgcc gacgtcagtg gagccgcccg aaataattag
    10681 ggtgtctatg ccgcgcgccc gtaacgctag atccaggtag gtttgataaa atgagctcca
    10741 gcgatatttc acgatgtagt aatcgtccgg gctcgggtca agctcggcga gggggtcgga
    10801 gcttttatcg ccggcgataa catgcatttt gggtttgctc acctcgccgt tgggccaggg
    10861 ggtcaacgaa ttgttggtat cggtgagcag cagcgccgtc gtggcattgt caggcctgtg
    10921 attgcccttg gcaaaaaata ccggcaggcc ggcggcgcgg ccggctttgc tgagtctcac
    10981 ggcattttga atccatggtt ttagaacccg ctgcttgccc ggttcgggcg cagcaacata
    11041 gccgttcaag atgtcgaaga agagcagccc agttttcttc ggttcaaaat agttgacgtt
    11101 cacttaagcc tcctaacgaa ttttgatccc ttgcttttct ttctcttgta ccactttcca
    11161 gccgtgctct gcataccatt cgatctgcct aaacagaccg gtcaggatgc gcacgtctcg
    11221 atcttccagc ccggtgcgtc ccaaaatgcg cctgaacgca aacagcatgt gctcagggtt
    11281 ttgcgaatca agaaagccga tttttagtag tgacgtgcgc atccggtcat acaaacgctc
    11341 gacatcttcg gcgcgcgcca gcggcagact ttctttcatc ggttcgccga gagatgcgag
    11401 ataaatctca tagagacaga tcatcgcggc ttgcgccacg ttcaatgact gatattccgg
    11461 atcggcggga atgttgatga gcaattgaca gtgcttcaaa tcttcattgc tgaggccgtg
    11521 gtcctcggga cccaagatta acgccgcttt gccggttgct gccgcggcaa cgatctctcc
    11581 ggcaacctcc cgcgggctcc ggctatgagc gcgatacaaa cctgcgcggc aagtcgtccc
    11641 gaccacgagc ctgcagtcgg caaccgcttc tcggagcgtc tcgtaaaaga tcgcattgtt
    11701 taaaatgtcc cggccatgca cggccatggc tcgcgcccaa aaacccttgg tgcgcgcttt
    11761 gccgacaatg gccaattcag gcacacctgt gttcttcatc gcgcgcgcga cagaaccgat
    11821 gttgcccgag ccgcgcgggc gaaccatcac cattcggatg ttctctagcg acatatacag
    11881 ttagtttgtt acagcacacc cagacaataa gccgttccag ccgttccacc gagattgaag
    11941 ccgttccaac ggttccagcc gtttttgccg aggacaagag ttccaagctg cgacagttcg
    12001 aaacattcag actcagcgat tgtagttacg acaaccggaa cttcttgaac atttggaatc
    12061 tttgggagta ttggaactgt cagaaccacg ggaacgattg gaaccttccg ttgcttattt
    12121 gcgaaaccgg cagagcttgt ttctttcaat cacggcgcga ttggcggcgt caaattcggt
    12181 ccggtagtcg aaatcttcgt gcatgatcga cgccgcgtca gcgacgtgct gggcgccgaa
    12241 gatatggccg atctcgtgga tcagagcgat gacttcaggt tgcggattgc tttgcgagcc
    12301 ggtgtagtga acgactctac tgaccggcac aaccgcatag ccgcccagtc cctgctggca
    12361 gttgccgatt cgatcgaccc gagggcggcc ggctctagta taccggctga ttccttcggc
    12421 agtaaaagcg atgacaagat cataatcgtg gttttttgtc tgtggaaaat cttgttcgag
    12481 cctggccaac aattcggggg tgccgggaat acgctcctcg agcggccacg cagaaacact
    12541 ttggctgacc aaacgaatgc cgaactcact ttcgtagtag tctgaagcgg cttcaaccag
    12601 gccccggact tcctcgggcc agcgggaatt tcttgccctc aggctaacgt cgcccacgac
    12661 cttgacccgg atgacccgtt ccggcgccgg agaactaatc gcggaacatg agacaagcag
    12721 cagtgcgcta aggatgcagc aaattatcgg tcggcgcgta atcatcggtc agcacgggca
    12781 catcttgatc aggcagcggc cggtcgaaat aagcgatgcc gatatcctga atcggattgg
    12841 gaaaaagacc cttatcaatg gacgttgccc gtttgacgat ctctttgatg tcgagtcgct
    12901 gcttgtcgcg ggtcgcgatc acaatgacgt tttgaatagt gtcgacgctg acgttatccg
    12961 ggcgtcgcgc ggcgaagaca taaatctggg gaaaaatttg cccctgcgtt ttcacgaacg
    13021 aacgcgcgat tttgccggag ggaccggtga cggcgctgat caggtttgcg acgatgatgc
    13081 cgttgggagt gagtttcttt tgcgccaatt cgaagaattc cttggtcgcc agatgaaagg
    13141 gcatggcatc ggtaaaatag gcgtcgagca tgatgatgtc gtattgatgc ggcgtccgcg
    13201 acagaaacat gcgtccatcc tgagcgtgca atctcaagtt attgctctcg cgcacgttga
    13261 aatgggtttt ggcgatttgg atcacttcag ggtcgagctc caccgcgtcg atttccaaag
    13321 agggaaattc tttgagcagc ttctttggaa tcgagccacc gcccaacccg atcagcgcca
    13381 tttttttggc atccggccga aatgcgactc ccaaggatgt atagcgcgaa taaatcagtc
    13441 tgagcgcggt cggatccttt agattcatgg cgctttgcaa ggtgcggtcg aagtacatgt
    13501 agcgcgcttc gtcgtcttct tcgacgcgaa tgcgatgata gaaagtttcc ttctcgacga
    13561 gtgtttttat ccgtgccaga gcgccgggtg tccaaggcat catgaaggat ccgagtatga
    13621 cagtgaccgc ggtgaccgcc cgggcaaggg taatgccccg caggcgcacc aaaggtacta
    13681 cgaatagcga caaacaaacc agagtaatcc cgagtccgtg aatgatgttg cgaacaccag
    13741 ccgcagggat cagataaaac gcggtcaaca gggtgccgaa aatactgccg caggtcgaaa
    13801 cggcatagag agtgccggcg gtgctgccga ccgttgaaag tgtcgttgcc gccagccgga
    13861 t
//
LOCUS       DASUVS010000060         4567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_458418, whole genome shotgun sequence.
ACCESSION   DASUVS010000060 DASUVS010000000
VERSION     DASUVS010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458418"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VF182_03455"
     CDS             complement(<1..184)
                     /locus_tag="VF182_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436160.1"
                     /translation="MFDRKALARRAKACICLGVSLVVFAAVAFPRTSRAATRDDLIEA
                     AKKEREVVFYTTTNLEE"
     gene            524..800
                     /locus_tag="VF182_03460"
                     /pseudo
     CDS             524..800
                     /locus_tag="VF182_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012053547.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            810..1223
                     /locus_tag="VF182_03465"
     CDS             810..1223
                     /locus_tag="VF182_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX6436161.1"
                     /translation="MKKNGMRPVPPGEVLNEEFLKPLGLTANALAKAIGVPPNRITAI
                     LKDERGITGDTAIRLGTFFKTSAEFWMNLQITYDLGNAEKALRRGLEKVSKGAGACCH
                     DMEDSRRKLKGLNAFARQTNVGARQRIKDSQDTKA"
     gene            1298..2275
                     /locus_tag="VF182_03470"
     CDS             1298..2275
                     /locus_tag="VF182_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436162.1"
                     /translation="MTETLSAPRKLVFLWLFYLLLPALAHAAAARPIVSVSSPGAGSS
                     YAMPFRIAEEHNFYVDEGLDVRIVSGVRTATSVQMLVGGTVDATQTVGPTTLAAILRG
                     VPLKIVMVFNDKPSYWLYSKKTIRSFADLKGSRISSSSPGSTNDRLLKLVLERNGIDW
                     KKDLQIIYIGTSDVVVRALLSGSIDAAVLSHPGNLIAKEAGYNQLATFENEVGALTGG
                     VAVHESFLSKRQDTAARFLRATLKGLKYFRTNREGSAKIMAKYMSLSHEAALKTHDAS
                     VPFYVSDGMISDDFQDKVLDFELKVIGTDKRISREAVFDFSIMKSLAAR"
     gene            2390..3430
                     /locus_tag="VF182_03475"
     CDS             2390..3430
                     /locus_tag="VF182_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6436163.1"
                     /translation="MAVFLANEDVEQLISMRDCIDSVEQAYGELGMGRAWNNPRIHTY
                     GQARDGATHFLKVFTGTVPALGYSILRIDSTMERNESGHATSRKIGSRSMGWLLLFSV
                     ESAALEAIIEDRALQRMRVGAMTGIAARHMARPNSRTVGIFGSSAQAGPQIEALAAVM
                     KLDTIKVYSPTRANREAFARRMDQTLDCRVVPVDGARDAVAGSDIVVVATNSNEPVFD
                     GGWLEPGTFVSSIVNSDKVLRRRDLDDESFRRAATIVVSTRDQIKNDEPIWLTEGLAS
                     GAVTWDRIWEIGELVCGKVPRREDHEITILKNNGLSVQFAGVGAAVMQRAKERRLGRE
                     LPSYLFEAAYRT"
     gene            3712..4497
                     /locus_tag="VF182_03480"
     CDS             3712..4497
                     /locus_tag="VF182_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6436164.1"
                     /translation="MLELYHGTTSVCAQKVRLTLAEKGLEWQSHLLDLNGDHLTPEYL
                     KINPNGVVPTLVHDGTIIVESTVIIHYLDDLFPGPSLMPRAPLDRTRAHLFKKLMDEY
                     IHPACIVYTFATANRGRFDGLSPEQRAEQYAKMPLQRQAEYKRAGVEEGIGSPAGKEA
                     TKSFMKLLKWIEQSTEKSAWLAGPDYSLADIAAIPYMMRLEMLRLARLWDHKPGVAAW
                     WERAKARPSYDKAFTQWLRPQDWQRYEKMPDPWPDVSKNLAAV"
BASE COUNT         1031 a         1315 c         1324 g          897 t
ORIGIN      
        1 cttcttccag gttggtcgtc gtgtaaaaga ccacctcacg ctctttcttt gccgcctcaa
       61 tgagatcgtc ccgtgtcgcc gcgcgggacg ttcgaggaaa cgcgacggca gcaaacacga
      121 ccagcgaaac accaaggcaa atacatgcct tcgcccgtcg cgctagcgct tttcggtcaa
      181 acatgttgct cccttgtttt cgcagctatt ggctctctct tatttgatct ttccttttcc
      241 ttcaaccgaa acgttcatcg tcgccccgtg cacgtcgagc tttcccgcac agttcggtga
      301 cggtgccacg ggtgtcacac ttgccccctc gactaattcc acggtattag ggaacgatgc
      361 cgtgccaccc gcgcccttca ggctgaagga ttgctctacc acgtaatggc ctgtggcaat
      421 gatgagcaga agatctttct cggcgaaacc gactatcagg ccttcaattc ccgagccact
      481 agaacgtttt acgtaaatcg taaaatctgc gaaattgtct tagatgatcc gaagcttcaa
      541 ggaccggaag acggaggatt ttttcggtgt agagaatgtc aagccgttct caggcttcaa
      601 gaagatggcg gagcgcaagc tggtgatgct ggacagcgcc gtgaaactcg gcgacctagc
      661 agcgagtccg gaaatagatt ggaaaagttg tcgggaaaca gggccggaca atacagtatc
      721 cggatcaacg atcaatggcg gatctgtttt acctggaaag acgacggtcc ctatgacgtt
      781 gaatagtgga ctatcattaa ggagtaacaa tgaagaagaa tggaatgcga ccggtgccgc
      841 cgggtgaagt tctcaatgag gagtttttga aaccgctagg cttgacggca aacgcgctcg
      901 cgaaggcgat cggggtgccg cccaaccgga tcacggcgat cctgaaagat gaaagaggta
      961 tcaccgggga cacggcaatc cggctgggaa catttttcaa aacctcggcg gaattctgga
     1021 tgaacctgca aataacctac gacttgggta acgctgagaa ggcgctgcgg cgagggttag
     1081 aaaaagtatc gaagggagcg ggagcctgct gccatgatat ggaagactca cgacgaaaac
     1141 tgaagggatt gaatgccttt gcgcgccaaa caaatgttgg cgcacggcaa cggatcaaag
     1201 atagtcaaga taccaaagcc tgaccctgtc gcctgccggt cataggatct ggctcagcac
     1261 gccgctgtct aataaaaaag atccgcatgg agactccatg actgagactc tctcggcgcc
     1321 aagaaaactt gtctttctct ggctatttta cctgctgctt cccgcgctcg cccatgctgc
     1381 ggcggcccgc ccgatcgtgt cggtcagcag tcctggagcg ggaagctcct atgccatgcc
     1441 ctttcgcatc gcagaggagc ataactttta cgtcgatgaa gggctcgacg ttcgcatcgt
     1501 gtccggcgtg agaacggcga ccagcgtgca aatgttggtc ggcggaacgg tcgacgcgac
     1561 gcaaaccgtc ggcccgacca cgctcgccgc cattttgcgt ggagtaccgc tcaagatcgt
     1621 catggtattc aacgacaagc cttcctactg gttgtattcg aaaaaaacca tccggagctt
     1681 tgccgacctg aaaggaagca gaatctccag cagctcgccg ggcagcacca acgaccgcct
     1741 gctgaaactt gttctggagc gaaacgggat agactggaag aaggatctac agatcattta
     1801 catcggcacc tccgacgtcg tcgtcagagc cctgctctcc ggatcgatcg atgcggcagt
     1861 cttgagccac ccgggcaatc tgatcgctaa agaagcgggg tacaaccagc ttgcgacttt
     1921 cgaaaatgaa gtcggcgcgc tgaccggcgg cgtcgcggtg cacgagtcgt ttctatcgaa
     1981 gcggcaagat accgcggcga gatttcttcg tgcgacgtta aagggactca aatattttcg
     2041 gacaaaccgt gaaggctccg ccaagatcat ggccaaatac atgagcctgt cgcatgaagc
     2101 cgcgctcaag acccacgatg ccagcgttcc cttctacgtc tccgacggca tgatctccga
     2161 cgatttccag gacaaagtcc tcgatttcga attaaaggtc atcggaaccg acaagcgcat
     2221 ttcgagagag gccgttttcg atttctccat catgaagtcc cttgcagcaa gatgaaacca
     2281 accaactcta cgcgggccaa gtccgtcgaa tgagatgatg agctttcccc cgatggttgt
     2341 tatgctaact tacggtagca actgagcgga tggtgttttc ggaggatcaa tggccgtttt
     2401 tctcgccaac gaagacgtcg aacaattgat ctcgatgcgc gactgcattg acagcgtcga
     2461 gcaggcgtac ggcgaactcg gcatgggccg cgcgtggaac aatccgcgca ttcacacgta
     2521 cggccaggcg cgtgacggcg ccactcactt tctgaaggtc tttaccggga ccgtgccggc
     2581 gctgggctat tcgatcctgc gcatcgactc gacgatggag cgcaacgaaa gcggtcatgc
     2641 gaccagccgc aaaatcggca gccgcagcat gggatggctc ctgctctttt cggtcgaaag
     2701 cgccgccctc gaggcgatca tcgaagatcg agccctgcag cgcatgcggg tcggcgccat
     2761 gaccggcatc gcggcccgac acatggcgcg cccgaacagc cgcaccgtgg gtattttcgg
     2821 ctccagcgcc caggcggggc ctcaaatcga ggcgctcgcg gcggtgatga agctcgacac
     2881 gatcaaagtc tacagcccga cgcgagccaa ccgcgaagca ttcgcccgcc gcatggacca
     2941 aacgctcgac tgccgggtgg tgccggtgga tggggcgcgc gatgccgtcg ccggctccga
     3001 catcgtggtc gtggcgacca attccaacga gccggtgttc gacggcggct ggctcgagcc
     3061 gggaaccttc gtcagctcta tcgtcaacag cgacaaggtg ctgcgccggc gcgatctcga
     3121 cgacgaaagc ttccggcgcg ccgcgacgat cgtggtcagc acgcgcgacc agatcaaaaa
     3181 cgacgagccg atctggctca ccgaaggact cgccagcggc gccgtgacct gggaccgcat
     3241 ctgggaaatc ggcgagctgg tgtgcggcaa ggtgccgcgc cgcgaggacc acgaaatcac
     3301 catcctcaaa aacaacggat tgagcgtgca gttcgccggc gtaggagcgg ccgtaatgca
     3361 acgcgccaag gaacgccggc tcgggcgcga gctgccgtcc tatctgttcg aggccgcgta
     3421 tcgcacatag ccgccgccgc tcatgggcgc ggcagaagtg attcaagcga ctgtcaacaa
     3481 aagatctgac ccctgctctt ctttcagaac tgacgggaag ctcagtgccg aacaccaaga
     3541 cattgcgcat cgcacgttcg ccgctcgggc gaacgacgta ttgcagggat ggtgtttcaa
     3601 gtagatcgag atatgaaagt ctggactatc atttctcctg atctccatgg ttttcattgt
     3661 cggcataacg taagttcatg tggcgctcgt gattcgataa atggaggaat catgctggag
     3721 ctttatcacg gcacgacttc ggtgtgcgcg cagaaggtgc ggctcactct cgcggaaaag
     3781 gggcttgagt ggcagagcca cctgcttgat ttgaacggcg atcatctcac gccggagtat
     3841 ttgaagatca atcccaacgg cgtggtgccg acgctggtgc acgacggcac gatcatcgtc
     3901 gagtccactg tgatcattca ttacctcgac gatctctttc ccggtccgtc gctgatgccg
     3961 cgcgcgccgc tcgaccggac gcgtgcccat cttttcaaaa agctcatgga cgaatacatc
     4021 cacccggcgt gcatcgtcta tacgtttgca acggccaacc gcggccgctt cgacggcttg
     4081 tctcctgaac agcgcgccga gcagtatgcc aagatgccgc tgcagcgcca agccgagtac
     4141 aagcgcgccg gcgtcgagga aggcatcgga tcgccggcag ggaaggaagc gaccaagagc
     4201 ttcatgaagt tgctcaagtg gatcgagcag tcgacggaaa aatccgcctg gctcgccggc
     4261 ccggactatt cgctggccga catcgccgcc attccctaca tgatgcggct ggagatgttg
     4321 cggcttgcac gcctgtggga tcacaagccg ggcgtcgcag cgtggtggga gcgcgccaaa
     4381 gcgcggccgt cctacgacaa ggcgtttacc caatggttgc gcccgcagga ttggcagcgc
     4441 tacgaaaaga tgcccgatcc atggccggat gtgagcaaga atctggcggc agtctgatcg
     4501 agaaactcga cgtagtttag ccgggcgcgc gaacgtcgcc cggcacttcg gcgtcgatgc
     4561 ccatcgc
//
LOCUS       DASUVS010000061         8386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_241128, whole genome shotgun sequence.
ACCESSION   DASUVS010000061 DASUVS010000000
VERSION     DASUVS010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8386
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241128"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VF182_03485"
     CDS             complement(<1..276)
                     /locus_tag="VF182_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436165.1"
                     /translation="MAQTRHSAELFREKYIKTATQTSMYIRGSPHRHSKNIATNSASQ
                     RSVRASPLSLLDSSTASQYSPGSMKQFLGLTTLLLLAVFLFGPAVGAA"
     gene            complement(612..1625)
                     /locus_tag="VF182_03490"
     CDS             complement(612..1625)
                     /locus_tag="VF182_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX6436166.1"
                     /translation="MTRLTLSFISAFNERVDPLMNGTVKAEGIDIVPTYSHPSETFWR
                     QLKFQEFDIAEMSMSSYLIARSRGFDMIALPVFPSRRLFHTELSYRTDSGIKEPGDLV
                     GKRLGVGEYQQTAALWTRGILEHEFGISQFKVHWYMERTEELSHGGATGFTPPPGISF
                     QRIPPDKSMASMLVNNELDAAAISSPWKNMPNVIGRSHRIKGSDGDWSKVKPLFPDRM
                     SEGARLFQKWGFLPVNHAYIIRGDIYKKYPWVAFNLYSGFVKAKQYFNERLLERIPTA
                     LFFGSEYLTTTRQMFGDDPYPYGVKANRKMLTALIDYSHEQGLTSKKLQIEELFAPST
                     LDL"
     gene            complement(1691..2704)
                     /locus_tag="VF182_03495"
     CDS             complement(1691..2704)
                     /locus_tag="VF182_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436167.1"
                     /translation="MTRLTLSFISAFNERVEPLMNGTVQAEGIELIPTYSHPAETFWR
                     QLKFQEFDVAEMSMSSFLIARSQGVDMVALPVFPSRRLFQTEISYHVDSAVGAPGDLA
                     GKRLGVAEYQQTAALWIRGILEHDFAVSQYKIHWYMERSEEMSHGGATGFKPPAGISF
                     NRIAPNKSLASTLLDHELDAAHIASPWVLRANALDRSSRITGSGADWSKIKPLFPDRM
                     AEAERFQKQHGFLPVNHTYIIRGDIYRKHPWVAFNLYNGFVRAKALARERLLESIPSA
                     LFFGPEYLKRTQDILGNDPFPYGIKANEAMLETITGYSHEQGLTPKKINYKEIFAQNT
                     LDL"
     gene            complement(2835..3521)
                     /locus_tag="VF182_03500"
     CDS             complement(2835..3521)
                     /locus_tag="VF182_03500"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6436168.1"
                     /translation="MATSKEFVDQLKQDIQAFPKIRIKHPFLKAICAGTATMDQIRAW
                     AIQDYQFRAAVPRIAMLRYLACNDPEIAQRLWGVVEEETRGMDTGSAGHNELAIRFAN
                     AIGLTQEQLENAELRPSTAAHLYYVELIIHTLPWFVVMSIQIGAEGTFGPAAAAIGHG
                     LIKNYKMKPDDVRFFTVHSEADEDHSSLAEDIAVRYLHSPHLQEQTRKHTFRRMELLY
                     DIWSIDGYEK"
     gene            complement(3615..4331)
                     /locus_tag="VF182_03505"
     CDS             complement(3615..4331)
                     /locus_tag="VF182_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6436169.1"
                     /translation="MSAYRTDVSGLDSSELKELKEKLITACHILDHEGVTDGYGHISV
                     RVPGADAFITISNVSPGCVTDDRLIMQDFAGNYLGGAKTPPNEWPIHACILKARPDVE
                     SVAHTHSKWSTIFSVLPVKLKPMHHYGKFLSAEGPPVYGGIGLVRTVERGNDLAQALG
                     DGPVVLMRAHGDTVVGESIEQVIERTTRLAMLGEWNHLALLHGEPNYLTAEELEVYNA
                     DQRFPMRGWEYFVSRLNGKR"
     gene            4497..5531
                     /locus_tag="VF182_03510"
     CDS             4497..5531
                     /locus_tag="VF182_03510"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436170.1"
                     /translation="MEVYSVGTGGRAMIWMFGLFLALVVYLLPTLSNAQEKFIASYAG
                     FAGFQAPLWAAKDFGFLAKYGVDTDLVMIPGSARGAQALLGGSIHFGQIDGTALISAI
                     NQGADLVFVASSLNRFPFSLVAQKNIREPKDLVGKKVGIVSLGGAHEVSLILALREWN
                     IPRASVTLLASGPAANRLIALASGALDATLLAPPETGEAARMGLSTLAHMTELKAAYF
                     PMNAIATRRSLLEKNRSAVKRFLQAYSEGIHQFMTQKQKSVALLTQRMKQKNPAVVEE
                     TYQYFASTFSSPPRMSHQGMRAAIDMLAQRSPEIKFDTNVSKYLDERLLDELEREGFF
                     EKMDPKMDRK"
     gene            complement(5721..6734)
                     /locus_tag="VF182_03515"
     CDS             complement(5721..6734)
                     /locus_tag="VF182_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436171.1"
                     /translation="MRVSRAASKHLIAATVASVLIFSGVTAFAQQTRLNVGYVGINSD
                     NVIAFIAKETGIFARNGLDVQLIYFGGGSTATMALISGETPITQTAGPGIVNAVLNGS
                     DAVMIAGGVTTLDYWMLSRPEIKTAEQLKGGAVAISRFGSASDFIVRYALQKLGLIPV
                     KEVAIIQVGPLPDRLAAMETKRVQATVLAPPAMYMAQKRGYNIIADIAALGLAYQATG
                     VGTTRKFIREHPDVVRRYVKAQVEAVHRFKTDRESSIRVLSKYLSLKEKDILDKTYDG
                     AIAENKLPAKQYPTLEGIKTILEPLKNDPKAKSAKPEDFVDMRFIKELDDSGYTASLY
                     KEK"
     gene            complement(6864..8111)
                     /locus_tag="VF182_03520"
     CDS             complement(6864..8111)
                     /locus_tag="VF182_03520"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEX6436172.1"
                     /translation="MYSIGRIFSITCALLLNSLMVSVRVFATDSTPRVFIIDGAALTI
                     AKQRTQHKDGILKPALAKLVRDADRALTIEPFSVTRKKIAPPSGDRHDYMSLAPYWWP
                     DPNTPNGLPYVRRDGKINPERYSIADRQNLENTVASVKTLALAYYFTANEDYAKNAAT
                     RLRVWFLDPLTRMNPNLKYAQGIPGRSDGRPAGIIETHNLPELIDAVALLAGSAAWNE
                     AHQRQLQGWFASYLTWLIESTEGRAEAQAENNHGTWYDVQIASFGLFTEKKDLAHRVL
                     GEIPRKRIARQIEPDGSQPLELARADSWRYSVFNLEALFDTASLGDHLGIDLWSFETG
                     DKRSIRQALDRLIPFATGEKQWPLREQSLWQPERLAPLLQRAALRTRDHSYGAALGKL
                     AAPSTDSRERLLYPMDEMASKAN"
     gene            complement(8135..>8386)
                     /locus_tag="VF182_03525"
     CDS             complement(8135..>8386)
                     /locus_tag="VF182_03525"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436173.1"
                     /translation="PLPEGVEQRIARISKAVYRALRIRGYGRIDLRLTPENEVVVLEA
                     NPNPNLDRDDEFAQSAIKGGLSYERLTQRILNLALQAAQ"
BASE COUNT         1775 a         2246 c         2356 g         2009 t
ORIGIN      
        1 ggcggcgccc actgctggcc caaagagaaa aaccgccagc aacaaaagtg tcgttaaccc
       61 gagaaattgt ttcatactcc cgggtgaata ctgtgaagcg gtagaggagt caagcagcga
      121 taaaggcgat gcgcgtacgg atctttgtga cgcgctgttc gtcgctatgt tcttcgaatg
      181 ccggtgcggc gaaccacgta tgtacatcga agtctgcgtc gcggttttga tatatttttc
      241 gcggaacagt tcggcggagt gtcgcgtctg ggccaccgac gtttcagtgt tgggacgttg
      301 gaaatgcatt cgtattagtt ataaccgaaa ttgcacgcat gatttcgctg gcacgtgcat
      361 tgcggaatcg tcgcgcagga ttgtttttat gtcaggccaa caggtctggc ggatagtcaa
      421 aggagggttt atgagcagct taaacaatca gaagggtgca atcggttgga tacttctttg
      481 ggttttaggc attccgatac cgatactttt actcttgttc gtggtccgcg gctgtacctg
      541 atcgcgagta aagatagagt caacaaccat gtgcctcgtt ggtttccgcg cgcatcaacg
      601 aggcactttt tttataggtc aagggtgctg ggcgcgaaca gctcctcaat ttgtaatttc
      661 ttcgacgtca gtccctgttc gtgagaatag tcgatcaacg ctgtcagcat cttccgattg
      721 gctttgacgc catatggata cgggtcgtct ccgaacatct gccgcgtcgt ggtcaagtat
      781 tccgagccga agaacagggc cgttggaatc cgttcaagta atctctcgtt gaagtattgt
      841 tttgctttga caaagcctga gtaaagatta aacgccaccc agggatactt cttgtagata
      901 tcaccgcgga taatgtaggc gtggttaacc ggcagaaatc cccatttttg aaacagcctt
      961 gcgccttcac tcatgcgatc cgggaatagc ggttttactt tgctccagtc gccatcgctt
     1021 cccttgatgc gatgggaacg gccaatgacg ttgggcatat ttttccacgg gctcgagatc
     1081 gccgccgcat ccaattcatt attcaccagc atcgaagcca tgctcttgtc cggcggaatt
     1141 ctctggaacg agatcccggg cggaggcgta aaaccggttg cgccgccatg gcttagttcc
     1201 tccgtccgct ccatgtacca atgaactttg aattgtgata tgccgaattc gtgttcgagt
     1261 atcccgcgtg tccataacgc cgcagtttgc tggtactccc caacccccaa tcgcttgccg
     1321 acgagatcgc ccggttcctt gatgccggag tccgtacgat aggagagttc ggtgtggaac
     1381 aggcgacggc tgggaaacac cggcaacgcg atcatatcga aaccccgcga ccgcgcgatc
     1441 aaatacgatg acatggacat ctcggcaatg tcgaactcct ggaacttcag ttgtcgccaa
     1501 aaagtttccg agggatgcga gtaggttggt acgatatcga tcccctcggc ttttactgtt
     1561 ccattcatca gtggatcgac ccgttcgttg aaagccgata taaaactgag agtcagtctg
     1621 gtcattgttg tcttcctgct gaaacgattg ccttgacgga ttcaagatcg catgagtcaa
     1681 gcgccgcgac tcataagtcg agagtgttct gggcaaatat ctccttgtaa ttgatctttt
     1741 tgggtgtcag cccctgctcg tgggaatagc ccgtaatagt ttcgagcatt gcctcattgg
     1801 ctttgatgcc ataaggaaac ggatcattgc caaggatgtc ttgtgtgcgt tttagatact
     1861 ccggcccgaa gaacagcgcc gatggaatgc tttccagcag cctctcacgc gccagcgcct
     1921 tggctctcac aaaaccgtta tacaggttga aagcgaccca cggatgtttt cgatagatat
     1981 cgccgcggat gatgtaggtg tgattgaccg gtaaaaatcc gtgttgcttc tgaaagcgct
     2041 cggcttcggc catccgatcg gggaaaagcg gcttgatctt gctccaatct gcgccgctgc
     2101 cggtgatgcg gctggagcgg tccagcgcgt tggctcgcag cacccagggg ctggcgatat
     2161 gcgccgcatc caactcgtgg tcgagcagag tcgaggccag actcttgttc ggggcaatgc
     2221 gattgaagga gattcccgcc ggaggcttga agccggtggc gccgccatgg ctcatctcct
     2281 cgctacgttc catgtaccag tgaattttgt attgcgaaac cgcaaaatcg tgttcgagaa
     2341 taccgcgtat ccaaagggcg gccgtttgtt gatactcggc aactcccaat cgctttccgg
     2401 ccaagtcgcc cggcgcccct acagcggaat cgacatggta ggagatctcg gtttgaaaca
     2461 agcggcggct cggaaatacc gggagcgcaa ccatatcgac gccttgtgag cgggcgatca
     2521 gaaacgagga catcgacatt tctgcgacgt cgaactcctg aaacttgagc tgtctccaga
     2581 atgtctccgc cgggtgcgaa taagtcggaa tcagttcgat gccttcggcc tgcaccgtgc
     2641 cgttcatgag tggttcgacc cgttcgttga aagcggagat gaaactcaat gtcagtctag
     2701 tcatctgtca acttcattag ctgatatcct ggcgcggttc gaggcgcact atggattcgt
     2761 cacttagtcg aaatgacatt gcgagccgcg tcatttcgat gacgcgaaag cgatcgagaa
     2821 atccctctgc gacattactt ttcatatccg tcgatggacc agatatcgta gagcagttcc
     2881 atgcggcgaa atgtgtgttt gcgcgtctgc tcctggagat gcggcgaatg gaggtagcgc
     2941 accgcgatgt cttcggccaa gctgctgtgg tcttcgtcag cctcggaatg aacggtgaaa
     3001 aagcgcacat cgtcgggctt cattttgtaa ttctttatca gaccgtgacc gatcgcggcc
     3061 gccgcggggc cgaaggttcc ttcggcgccg atctggatcg acatgacgac aaaccaaggc
     3121 agcgtgtgaa tgatcagttc gacgtaatat aaatgcgccg ccgtggacgg ccgcaactcg
     3181 gcgttttcaa gctgttcttg agtaaggcca atggcattgg caaagcggat tgccagctcg
     3241 ttgtggccgg cgctgcccgt atccatgccg cgggtttcct cctcgaccac accccaaagc
     3301 ctctgcgcga tttccggatc gttgcacgcc aggtagcgca gcatggctat gcgcggcacc
     3361 gcggcgcgga attgataatc ctggatcgcc caggcgcgga tctggtccat tgtcgctgtg
     3421 cccgcgcaaa tggcctttag aaacggatgc ttgatgcgga ttttcggaaa cgcctgaata
     3481 tcctgcttca actgatcaac gaattccttg gatgtggcca tcttgttcct cccactacgt
     3541 ttattgcttt gttggagtaa tggactggtg gagtgttgga aagcccagtc ctccagaaac
     3601 tcatcactcc gattctatct ttttccgttc agccggctaa cgaagtattc ccaaccgcgc
     3661 atcggaaaac gctgatcggc gttgtaaacc tccaattcct cggctgtcaa atagttcggt
     3721 tcgccatgga gcagcgccag atgattccat tctccgagca ttgcaagcct ggtggtgcgc
     3781 tcgataacct gttcgatcga ctcgccgact acggtatcgc cgtgagcgcg catcaacacc
     3841 accgggccgt cgcccaaagc ttgggcgaga tcgttgcctc gctccaccgt acgaaccaag
     3901 ccgatgcccc catacaccgg cggtccttcg gcggacaaaa atttaccgta gtggtgcatg
     3961 ggtttgagct tgaccggcag cacgctgaaa attgtgctcc acttcgaatg tgtgtgcgcg
     4021 acgctctcga catcgggacg cgcttttaaa atgcacgcgt gaattggcca ctcattcggc
     4081 ggcgttttgg cgccgccgag ataattaccg gcgaagtctt gcatgatcag tcgatcgtca
     4141 gtgacacagc cggggctgac gttggaaatg gtgataaacg catctgcgcc gggaacgcgt
     4201 acgctgatgt ggccgtaacc gtcggtcacg ccttcgtgat cgagaatgtg acaggcggtg
     4261 atgagcttct cctttagttc tttgagctcg gaggaatcga ggccggatac atcggtgcga
     4321 tatgcgctca tgaaatctcc ttagagtaat ctccaacaag cctgcgatca gcggttgtgg
     4381 agaaaatcta ttgcgccgcc cgtccacctg tcaagcttcg cttgacaaat aactgccgag
     4441 aattggataa ctcgcagcag cttgcttgac caggaatgtt cgagaagcgg agtcagatgg
     4501 aagtttattc ggtcggtacc ggggggcgcg ctatgatctg gatgtttggg ttgtttctgg
     4561 ctttggtcgt ctacttgctg ccgacgctca gtaatgcgca ggagaaattt atcgccagct
     4621 atgccggatt tgccggattt caggcgcctc tttgggcggc aaaggatttc ggctttctcg
     4681 caaagtatgg cgttgatacc gatcttgtca tgattccggg ttcggcgcgg ggagcacagg
     4741 ctttgctcgg cggcagcatc cacttcggcc agatcgacgg caccgcgctg atctcggcca
     4801 tcaatcaagg cgctgacctg gtgttcgtcg cctcatccct gaatcggttt ccattcagtc
     4861 tggtagcaca gaaaaatatc cgcgagccca aggacctggt cggcaaaaag gtcggcatcg
     4921 tgagtttagg cggcgcacac gaagtgtccc tcatcctggc gcttcgagaa tggaacatcc
     4981 cgcgcgcttc ggtgaccctg ctcgccagcg gtccggcggc gaatcggctg atcgctctgg
     5041 caagcggcgc tttggacgcg acactgctgg cgccgcccga aaccggcgag gccgcgcgca
     5101 tgggattgtc gaccttggcc catatgacgg aacttaaagc ggcctatttc ccgatgaacg
     5161 ccatcgcgac acggcgctcc ttgctcgaaa agaatcgcag cgcggttaaa cgatttctcc
     5221 aggcttatag cgagggaata caccagttca tgacccaaaa acagaaatcc gtggcgcttt
     5281 tgactcaaag gatgaaacaa aaaaatcccg ccgtcgtcga ggagacgtat cagtattttg
     5341 cttcgacgtt ttcttccccg ccgcgcatgt cgcaccaagg gatgcgcgcc gccatcgaca
     5401 tgctggcgca gcgttctccc gaaattaaat tcgataccaa cgtaagtaaa tatctcgacg
     5461 agcgcttgct cgacgaactg gagagagaag gtttctttga aaagatggac ccaaaaatgg
     5521 accggaagta ataggcggca ttcagtccta agcgcctgcg cctatcgatc ttgtccgatg
     5581 tagtgtctat gggaagcgtc ggccggctga tctgcctgtg ttgggaatgg ggagagttaa
     5641 aataagagcg ctgccaccaa gtcggcgata acgaccgttg ctgtcctcac atccggtatt
     5701 tggaaaagcc tcttcgacgc ctacttttct ttgtacaagc tggctgtata gccgctgtca
     5761 tcgagttctt tgataaaccg catatcgaca aagtcttccg gtttggctga tttggctttc
     5821 gggtcattct tcagcggctc caggatggtt ttgatacctt cgagggttgg atactgtttg
     5881 gccggaagct tgttctctgc gatagcgccg tcgtatgttt tgtcgaggat gtccttttct
     5941 ttcaagctta agtattttga tagcacgcgg atgctgctct cccgatcggt cttgaatcga
     6001 tgcaccgcct cgacttgcgc cttcacgtat ctccggacaa cgtccgggtg ctcgcgaatg
     6061 aacttccgcg ttgtcccgac gccggtcgcc tgataggcga ggcccaatgc ggcgatgtcg
     6121 gcaatgatgt tgtagccgcg tttctgggcc atgtacattg ccggtggcgc cagcacggtc
     6181 gcttgcacgc gcttggtttc cattgccgcc aagcggtccg gcaacggacc gacctggata
     6241 atggcgactt cctttaccgg tatgaggcca agtttctgca atgcataacg cacaatgaag
     6301 tcggaagccg acccgaaccg gctgatcgca actgcgccgc cttttagttg ctcggcggtt
     6361 ttgatctcgg ggcgactcag catccagtaa tccaatgtcg taactccacc ggcaatcatg
     6421 acggcatcgg agccgttgag gacggcgtta acgatgccag gcccggctgt ttgcgtgatc
     6481 ggcgtctctc cggaaatgag cgccatggtg gcggtgctgc cgccgccaaa gtagatgagc
     6541 tgcacgtcta gtccattcct ggcgaagata ccggtctctt tcgcaatgaa ggcaatgaca
     6601 ttatcggaat tgatcccgac atagccgacg tttagtcgag tttgttgggc aaaagccgtg
     6661 acgccggaaa agatgagtac ggatgcaacc gtggcggcaa tgagatgttt tgacgctgcc
     6721 cgacttactc gcattcccgt tcctccatgt tcgttgattg gcgtagccct gatacaaaat
     6781 gtagttgaac aagccgtggt gacgcattca atgaaagtgc ggtaagccac ggcattggct
     6841 gaccagatcg aattcctcgg actttagttt gccttcgaag ccatttcatc cattggatat
     6901 aacaggcgct cgcgactatc ggtagacggc gccgctagtt tgccgagggc agctccatag
     6961 ctgtggtccc gggtgcgtag agccgcgcgt tggagaagcg gtgcaaggcg ttcgggctgc
     7021 cagagagatt gctcgcgcag gggccattgc ttttcaccgg tggcaaacgg aatcaaccgg
     7081 tccagggctt ggcggatact ccgtttatcc ccggtctcga agctccagag gtcgatccct
     7141 aaatggtcgc ccagagaagc cgtatcgaat agtgcctcga gattgaatac cgagtagcgc
     7201 cacgaatcgg cacgcgccag ctccagcggc tgactgccat cgggctcaat ttgcctggcg
     7261 attcgcttgc ggggaatctc accgagcacc cggtgagcca aatctttctt ctccgtgaaa
     7321 agaccgaacg aagcgatttg gacgtcgtac catgtgccgt ggttgttttc cgcctgggcc
     7381 tcagcgcgcc cttcagtgct ctcaatcaac caggttagat aggatgcaaa ccacccttgt
     7441 aattgccgtt gatgcgcctc gttccaggct gccgatcccg cgagcagcgc caccgcgtcg
     7501 ataagctccg ggaggttgtg agtttcaatg atacccgcag ggcgtccgtc acttcgtcca
     7561 gggatgccct gagcgtattt caaattcgga ttcattctcg tcagcggatc caaaaaccaa
     7621 actcggagcc tggttgcggc gtttttggcg taatcctcgt ttgccgtgaa atagtacgcc
     7681 agcgccagtg tttttaccga agccaccgtg ttctccagat tttgccgatc ggcgatgctg
     7741 taacgctcgg gattgatttt tccgtcgcgg cgcacatagg gaagaccgtt gggagtattc
     7801 gggtcgggcc accagtacgg cgcaagactc atgtaatcgt gtcgatcgcc gctcggcggc
     7861 gcgatcttct tgcgcgttac cgaaaacggt tcgatggtga gagcgcgatc ggcatcccgg
     7921 acaagctttg ccagtgctgg cttgagaatc ccgtccttat gctgcgtccg ttgctttgcg
     7981 attgtcaggg ccgcgccatc gatgataaaa acgcggggcg tcgagtccgt cgcaaagacg
     8041 cgaacggaaa ccatcagact gtttaagaga agcgcgcagg taatgctgaa tatgcggccg
     8101 atggaataca tgagtccccg tagccttgcg ccagctactg agcggcttga agcgcgagat
     8161 tcaatatgcg ctgggtgagt cgctcgtaac tcagcccgcc cttgattgcc gactgggcaa
     8221 actcgtcgtc gcgatcgaga ttcggattag gattcgcttc cagaacaacc acttcatttt
     8281 cgggtgtcag gcgcaggtcg atgcggccat aaccgcggat gcgcagcgct cgatacaccg
     8341 ctttcgagat gcgcgcaatg cgttgctcga cgccctcggg aagtgg
//
LOCUS       DASUVS010000062        18651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1769871, whole genome shotgun sequence.
ACCESSION   DASUVS010000062 DASUVS010000000
VERSION     DASUVS010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 18651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18651
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769871"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VF182_03530"
     CDS             complement(<1..397)
                     /locus_tag="VF182_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HEX6436174.1"
                     /translation="MNIVVCGKVIPASSVTIEIDSNTKRMRRKGVPHELDPAAASAVE
                     EGLRLTEKHGGTVTLITMGISDATIGIRNALAMGVASAVHILDDAVAGSDTLGTAKIL
                     AAAIKKQPFDLVLCATESSDSYSGIVPGQI"
     gene            complement(502..693)
                     /locus_tag="VF182_03535"
     CDS             complement(502..693)
                     /locus_tag="VF182_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436175.1"
                     /translation="MQPAPNTEALDQQFKEFIQALQKAVGTAMPHLKDDPKIVDQAIA
                     DCKEILDVVLEGDVKEWVS"
     gene            complement(704..1426)
                     /locus_tag="VF182_03540"
     CDS             complement(704..1426)
                     /locus_tag="VF182_03540"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEX6436176.1"
                     /translation="MFTDKIDLKDFEKQDPEYQDLLKRVLAIQADCEIGGPNLYVKNI
                     LPSAPTQLDQLIVARTAAEEMDHYRKMARLAGDIGLDVSYVLSRSNQERYVEAFRGSI
                     DTWEDYAVFGFLIDRVGRFQLEEFIGCTYLPLARIVEDPDVIREEEGHIDFGTNKTAE
                     YAAKGGEAKERIQKAVDYWYVKGLDMFGNSKSYRSERYMQWGLKRRPNAVARQQYKNE
                     VDGLLAKMDLNIPDSNIGRLYT"
     gene            1564..2913
                     /locus_tag="VF182_03545"
     CDS             1564..2913
                     /locus_tag="VF182_03545"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX6436177.1"
                     /translation="MLAPESLALLTDLYQLTMAQAYFNEHKTERATFSLFIRSYPLNR
                     AFFISAGLHDVLKFLEDLAFDRAALEYLSRQKFFTHDFLKFLEELRFTGDVWALPEGR
                     LFFKDEPILEVNAPIIEAQLVETFIINQINLQSLIATKAARCVLAAGKRAVVDFALRR
                     THGIDAGIKVARSSYLAGFAGTSNVLAGKKYEIPTVGTMAHSFVTSFGEESDAFRSYA
                     ASFPENAILLIDTYDTIAGARKAVEVAEEMAARGQRLRGVRIDSGDLAGLAIKVRKIL
                     DEAGFSDVAIIGSGGLDEYDLSEFSRARIPFDSYGVGTKMGTSSDAPWSDMAYKLVEY
                     GDKPVLKRSPGKSSWPGAKQLFRQRNNQGQLERDIVGLRHEGIADTEALLRKVMEGGK
                     IVGTLPTLAQSRAGLAEELGRLADLTKAIRDPVLYPIEFSPELVRLREETERKLIKP"
     gene            complement(3064..4056)
                     /locus_tag="VF182_03550"
     CDS             complement(3064..4056)
                     /locus_tag="VF182_03550"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX6436178.1"
                     /translation="MSGGQRRSVILGTGSELPSKVITNRDLEKMVETSDEWITVRTGI
                     KERRILEEGKGNADMAFHAATRALEDAGLNPDDLDAIIMGTVTADYPFPSSACVLEDM
                     LGARRVFSLDVGAACSGFLNALSVADSFIRLGKIRNALVVGSDALSRLLNWQDRTTCI
                     LFGDGAGAVVLGASDNGNGILSTKLRTDGSYAKTLYVPAGGSLKPANCKTVERNEHTI
                     TMNGKEVFKIAVRSMEDISREALEEAGVGIDQVSLVIPHQANRRIIVALAERLGVSMD
                     KVMVNLEKYGNTSAASIPVALDEAKRQGRIQKDNIVLLNAFGAGFAWGAAVVKF"
     gene            complement(4053..5306)
                     /gene="fabF"
                     /locus_tag="VF182_03555"
     CDS             complement(4053..5306)
                     /gene="fabF"
                     /locus_tag="VF182_03555"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX6436179.1"
                     /translation="MNTAKQRRVVLTGIGLVTPLGTGVEKNWDALITGRSGIRRISRF
                     ANVDAYPSQIAGEVPDFSPADFIEPKEIKKMDLFIQYAVAAAAMAMMDSGLKIDAELA
                     EQVGVIIGVGLCGIDTIESTKEAILQGGPRKISPFFIPKVISNLAPGQIAIRHGAKGI
                     NLTPTSACASGTHAIGEAYHLIRRGLQDAVITGGAESAITPLAVGGFAAMKALSTRNN
                     EPERASRPFDRDRDGFVIAEGSGVLILEERERALERGAKIYAEVVGYAANGDAHHMTA
                     PAPEGEGAARCMRLALKDAGLAPTDVDYINAHGTSTEYNDANETMAIKRVFGEQAAKL
                     AVSSTKSMTGHLLGAAGAVEGVFSALTLYHGLIPPTINYDNPDPQCDLDYVPNQARKA
                     DVKTVLSNSFGFGGTNACVIMRRAE"
     gene            complement(5384..6391)
                     /locus_tag="VF182_03560"
     CDS             complement(5384..6391)
                     /locus_tag="VF182_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX6436180.1"
                     /translation="MIDREFLKHMPGRGHPERPERLRVLLDLAESLDPARFQLLPGRA
                     ASISEIELCHDPKYIKLVESTASLSQYALDGDTITSQDSFAVALHATGGFLRLLDSVA
                     AGESSNGFALVRPPGHHALRSRAMGFCLFNTIAVGAQYLKSAHQAERIAIVDWDVHHG
                     NGTQDAFYDDPSVLFISTHQYPFYPGTGGVAEVGLRQGAGFTINVPLPAGCGDAEYLR
                     VFRDIVIPGIKRFRPQWILVSAGFDPHRQDPLAGMDVTEAGFSQMARAMLQLAAVYCD
                     GRIAFLLEGGYDLIALRSSVAGVLEVLQHSSDGKFPSDVRADKLDPLIRALLRTHDQF
                     H"
     gene            6595..6870
                     /gene="tatA"
                     /locus_tag="VF182_03565"
     CDS             6595..6870
                     /gene="tatA"
                     /locus_tag="VF182_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX6436181.1"
                     /translation="MFGIGMPELLLILALALIVLGPKKLPELARALGKGMAEFRKATD
                     DLKDEFRQLEEEPSESWTQTAKKDDPPLTKKAEVSAASPAPANEPRE"
     gene            6905..7618
                     /gene="tatC"
                     /locus_tag="VF182_03570"
     CDS             6905..7618
                     /gene="tatC"
                     /locus_tag="VF182_03570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX6436182.1"
                     /translation="MPLASHLDELRSRLIRSVLAVAIAFVLCFYFADEIFSALAEPLR
                     SLPIPDLILIGTAVTEAFFTKMKISFFAALIVALPVLLWQGWQFVGPGLYEHEKRYTR
                     SFVLFGSFFFLAGVAFCYGIVLKKGLAFMLKRYQVLQIQPAIQIGDYLSMVARLVLAF
                     GVMFQLPVLTFFLARVGLVDHRLLIHYLGYAVIGMAIISAVLTPPDLISQILLMVPLI
                     LLYGVSIAVAYAARPRKMQ"
     gene            complement(7675..8226)
                     /locus_tag="VF182_03575"
     CDS             complement(7675..8226)
                     /locus_tag="VF182_03575"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HEX6436183.1"
                     /translation="MIPKKCFFTKGVGVHKEKLASFELALRSAGLAYCNLVLVSSIYP
                     PFCKRISKEEGVKLLRPGEIVFCVYDRESTNEPNRLVAASVGVAIPADQEQHGYLSEH
                     HSFGETEEKAGDYAEDLAASMLATTLGIEFDPDTAWDERENLFKMSGKIVRTSNITQS
                     AIGNKDGLWTTVFAACAFVNDDN"
     gene            complement(8295..8798)
                     /locus_tag="VF182_03580"
     CDS             complement(8295..8798)
                     /locus_tag="VF182_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX6436184.1"
                     /translation="MAFIAKLGEQTYTVDIDEVGKSVYKVSIDGNEFLIDGKKTGRTN
                     YSLIVDNRSFEIEVDHMEDEYQVLVDGRNYHVNLLDERRVRVGGTQSGIELQGRQRVS
                     VPMPGKVIAVLVAEGDTVEKGQGLVIVEAMKMENEVRSPISGEVKEIKVKSGETVEGG
                     AVLLIVE"
     gene            complement(8809..10350)
                     /gene="accC"
                     /locus_tag="VF182_03585"
     CDS             complement(8809..10350)
                     /gene="accC"
                     /locus_tag="VF182_03585"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX6436185.1"
                     /translation="MFKRILIANRGEIAVRIARACRELDIETVAVYSEADREALHVKY
                     ADYAYAIGPAPSVQSYLNIDRIIEVAKRSHAEAMHPGYGFLAENSQFAKRCLAEGIVF
                     IGPSPEVIDQMGDKVKARAIMKAAGVPVVPGSDGILTSEEQVLSTLEAIGYPCMLKAV
                     AGGGGKGLRLVRSRREVSSAYRAVGSEAASSFGDPRLYVEKYIEKPRHIEIQILGDNY
                     GRVLHLFDRECSIQRRHQKVIEESPAPGLDDRTRRRIGRIAIQGARAVRYVGAGTLEF
                     LLDQEQNFYFLEMNTRLQVEHAVTERVIGIDLVKAQIEIAAGGYLPWRQRHITQTGHA
                     IECRIYAEDAENDFMPCPGKIEGLRLPEGLGLRNDCGVYEGAEVPIYYDPMIAKLITW
                     GENRVEAILRMRRALREYQVRGIKTNISFHQWILRHPNFMSGKVHTGFIDQEYHSTSK
                     EEVFPHKEIALASAAIAAMHREQESTLGLLARGAAEPSKWRERGKRNALRLQPPFHAR
                     GWRRS"
     gene            complement(10399..11949)
                     /locus_tag="VF182_03590"
     CDS             complement(10399..11949)
                     /locus_tag="VF182_03590"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX6436186.1"
                     /translation="MTINNNFERLRELNQKAEAGGGELRARRQHEQGKLLARERLNVL
                     LDSGSFVELDRLRTHGCTDFQMQQQKFLGDAVVTGYGAIDGRLVYVYSQDFTVFGGSL
                     SGVVAEKICKIMDLAMKNGAPIIGINDSGGARIQEGVLSLAGYGEIFLRNVKASGVIP
                     QISAIMGPCAGGAVYSPAITDFVYMVKTNSYMFITGPDVIKTVTHEEVTKDELGGAET
                     HSAKSGVAHFTANGEKECLLMIRELIAFLPSNNQEDPPFHPTNDDPLRRDKKLRDLVP
                     DNPNRPYDMKDLIGAVIDEGYFYEVQRDFAPNLLIGFARLGGRPVGIVANQPAALAGC
                     LDINASLKGARFIRFCDCFNIPVITFVDVPGFLPGTSQEYDGIIRHGAKLLYAYAEAT
                     VPKITIVTRKAYGGGYIVMSSKHLAGDINLAYPTAEIAVMGPEGAINIVFREALQQAE
                     DSGKARDEMTQEYRNTFANPFKAAEFGYIDSIIYPEDTRPTLIRALEMLKNKQDYNPP
                     KKHGNIPL"
     gene            12130..13326
                     /locus_tag="VF182_03595"
     CDS             12130..13326
                     /locus_tag="VF182_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX6436187.1"
                     /translation="MLQFRSNRTNREHAASLNTADRSLKPDDALLLVASSEGDSNMLY
                     AVGFFVPDPFIFFQHKKKKYVVMSDLEVDRAKRQATVDQVLSLSDYQRKLRKADSPPT
                     MIGILELLFRERGIRSLKVPANFSALLADQLRDKGFNLEITPDPFFPEREIKRNQEVR
                     HITESLHVAKLGLDAGIRMLKKTKTGHDGYLYLNGSKLTSETLKTAVNTTIMAQGWLP
                     SHTIISSGNQCVDPHHEGTGPIKANTSIIFDIFPRSQKTGYFGDLSRTVVRGRASDKL
                     KKIYATVQAGQKIGFDLIRDGVNGKEVHQKILDLFAREGFPTGQIKGRMQGFFHGTGH
                     GLGLDIHEAPRIAPVDATLKSGHVVTVEPGLYYLGVGGVRLEDVVLVTDKGNKNLTDC
                     PQFLEI"
     gene            13393..14145
                     /locus_tag="VF182_03600"
     CDS             13393..14145
                     /locus_tag="VF182_03600"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX6436188.1"
                     /translation="MQQTKISEWLEALDRPNKQMLRAAVEGLIAALPDSPETGRNLLL
                     LLRDPERKNRWPIAYILAHLPQPPDDSLEILLDALDHPDPDVRWAVILLLARLTKEQE
                     SIVQLTTSVIATGTATQRRMVIYYLKELKRRSEAVRQALLAALNDPDPTVRVAAVISL
                     KDQDVDSNGKTTLLNLLMRDADVRVQHAAAITLASLGSPSEEFLSALNHATQSAQEPL
                     KKAALAAQRILQEKKEVRPIRQLTSPVRRTSS"
     gene            complement(14388..15161)
                     /gene="pgeF"
                     /locus_tag="VF182_03605"
     CDS             complement(14388..15161)
                     /gene="pgeF"
                     /locus_tag="VF182_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX6436189.1"
                     /translation="MKINWLKVPQWENDPDLLHGFMGRQGGQSVGAYAGLNVSYRVGD
                     DPKTVSQNVCDMKLAVGIHDGRIVTMKQVHGDHLIEVKGTSVKEAGEADGMVTGARNT
                     FLGVLTADCVPILLLARQQKIVAVVHAGWRGTLKGIAGKTVRLFLESYSVLPSELEVA
                     LGPSIDACCYEIGRDVAQPLIEQWAELTTSSIENRDNKSYLDLRNLNRAILHRAGIPQ
                     SQIFRVGPCTSCAKDEFFSYRREKKETGRQISFIGWRSE"
     gene            15286..>18651
                     /locus_tag="VF182_03610"
     CDS             15286..>18651
                     /locus_tag="VF182_03610"
                     /inference="COORDINATES: protein motif:HMM:NF006386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HEX6436190.1"
                     /translation="MTNGRYIGTLKQVGFFAFFCTQFLGAFNDNFYKIVVSLVALNAA
                     TPESDSLYVDLIAFLFILPSALFSGYAGHLADAYSKRSILVAVKCFEIVIMGMGFAAF
                     LSGYVEPMLVIVFLMGLHSAFFSPAKYGILPEMLPETELSRGNGLLEMSTFMAIILGV
                     SLGGAIYSIWKDQLPLVGLFMVAIAVLGTLTSLKISPVPASGSTRHFNLNPFGEIWHG
                     IKRLYPDKPLRLTVIGISYFWFLGALVQLAMLFFGKELLGLDELHISLLGTFLAIGIG
                     LGSLAAGKLSGDKIELGLVPLGSISMGVCLLLVSYSGSSYFKTSAFLVLLGFSGGLFA
                     VPLNALLQHRSGREEKGQLIATNNFFNTIGIILAALVHWILNSALQLSPDWIIFIIGV
                     LTLIATAIVLVVLPDYFVRLVAWLLTHTLYRIRIAGEENAPVHSPALLVSNHVSFADA
                     LLIGACLPGFVRFMLHRDYYENKWLNWFFRLMKSIPIRATHRRDVVNSLKRARQELEA
                     GHVVCIFAEGAITRTGHILPFKRGFEKVVEGTSVPIIPVHLDQVWGSVFSFHGGRFFW
                     KWPKFFPYPVTVSFGKPLPANATATEVRQAVLELASEAARYRRKPTDLLPAKFVQRAK
                     RRWFSFCMADTTGTELTFGKALIGSMLLCRWVSEHSPKERMVGVMLPASVAGALANLA
                     IMLAGKVPVNINFTAGQDATLSAIKQCGIKTILTSRLFLNKAGLQELPGMVFLEEVRK
                     TFTPALKLVTAAKAAVLPAVYLNRNVTNSAAPDDLATVIFSSGSTGMPKGVMLSHSNI
                     ISNIEGICQVIQFTKKDRIMGILPLFHSFGFTATFWLPLLAGFGVVYHPNPMDAKTIG
                     ETVQKHQATLLISTPTFYAGYLRRCSPEEFASLRYVIAGAEKLREQIARAYKEKYGID
                     LLEGYGCTELAPVVSVNIPDVIDNDEKQIGHKPGSVGHPIPGVAVKVIDPETERPLDC
                     GQEGLLLVKGPNCMLGYLGQPEHTAQVFRNKWYVTGDIATIDEDGFIRITDRMSRFSK
                     IGGEMVPHMRIEEVINEILGSAAAVVTALPDEQRGEKLIAFYTHNGISRDELWNRLNQ
                     STLPKLWIPKREDLFILESIPLLGSGKIDLKQIR"
BASE COUNT         4354 a         5059 c         4703 g         4535 t
ORIGIN      
        1 cgatctggcc gggaactatg ccagaatagc tatcactgct ttctgtggcg cagagaacta
       61 aatcgaacgg ttgtttcttg attgccgcag cgagaatttt tgcggtaccc agggtatcag
      121 aacccgcaac cgcgtcatcc aagatgtgga ctgccgaggc aacccccatg gccaaagcat
      181 tacgtattcc gatagtggca tcactgatgc ccatagtaat gagggttacc gtaccgccgt
      241 gtttttctgt tagacggagg ccttcctcaa ctgcacttgc cgcagcggga tccaactcat
      301 gaggcacacc tttacgccgc attcttttcg tattggaatc aatttcgata gttaccgagc
      361 tagccggaat tactttaccg cagacaacaa tgttcaatgt tcatcttctc ctgaaccacc
      421 ccggggcatg atagacaaag gcgcctaact gctgcattag gcgccttcgt gatttgaatc
      481 tagatgccct aaatcggaag tttaagacac ccactctttc acgtcacctt cgaggacgac
      541 atccaaaatc tctttacaat ccgcgatcgc ctgatcgacg atcttaggat cgtctttaag
      601 atggggcata gccgtaccaa ctgctttttg aagggcctga ataaactctt tgaattgctg
      661 atccaatgcc tcagtattcg gtgctggctg cataatccta cctttacgta taaagacggc
      721 caatattaga atccgggatg ttgagatcca tcttagcaag aagaccatcc acttcatttt
      781 tgtattgctg acgcgcgacc gcattcggtc gccttttcaa tccccactgc atgtaccgtt
      841 cagacctata cgacttactg ttaccaaaca tatcgagccc tttcacgtac cagtagtcaa
      901 ccgccttttg gatacgctct ttcgcttctc cgcccttggc ggcgtactca gcagtcttat
      961 ttgtcccaaa gtcaatgtgc ccctcttctt cccgaatgac gtctggatct tcgacgattc
     1021 gcgccagcgg cagataggtg catccgatga actcctcaag ttgaaagcga cccacgcgat
     1081 cgatcaaaaa tccgaacacc gcataatctt cccacgtatc aatcgaaccg cgaaacgcct
     1141 ccacataccg ttcctggttg gaacggctta acacatatga cacgtcgagg ccgatatccc
     1201 cggctaaccg agccatcttt ctgtagtggt ccatctcctc agcagcggtg cgggcgacaa
     1261 taagctgatc cagctgagtc ggcgccgacg gtaaaatatt cttcacgtaa agattgggcc
     1321 caccgatctc gcagtcggcc tgaatcgcaa gaacccgctt cagtaagtcc tgatactccg
     1381 gatcttgttt ctcaaaatct ttgaggtcaa tcttgtcagt aaacatttgt tcgggcctcc
     1441 catcgctcgc taattatgcg caaatattat agaaattttt aacaaaatgg acttcttaaa
     1501 tcaataagcg ctgcagtccg gctgttctta cgcgtgtaat cgatccagca ggagattgag
     1561 cttatgttag cacctgaatc actagctctc ttgacggatc tttaccaatt gacaatggcg
     1621 caagcttatt tcaacgagca caaaacggag cgagctacct ttagcctttt tatccgatcc
     1681 tatccgctaa acagggcatt cttcatttct gcaggcttac atgatgttct caagtttttg
     1741 gaggaccttg cattcgaccg ggccgctctg gagtatctct ccagacagaa attttttacc
     1801 catgattttt tgaagttctt ggaagaatta cgattcaccg gggacgtttg ggcattgccc
     1861 gaaggtcggc tgttttttaa agacgaaccc atcctggaag tcaatgctcc catcatcgaa
     1921 gcccaactgg tggaaacgtt tatcatcaat cagatcaacc tacagtcact gattgccaca
     1981 aaggcagctc ggtgtgtcct tgccgcgggc aagcgggccg ttgtcgattt cgctctacgc
     2041 cgcacgcatg gaatcgatgc cggtataaaa gtcgcgcggt cgagttatct tgccggattc
     2101 gcaggcacca gcaatgttct agcaggtaag aaatatgaaa ttccaacggt cggaacaatg
     2161 gcccactctt ttgtcaccag cttcggagaa gaaagcgatg cttttcggag ttatgcggca
     2221 agttttccag aaaatgcgat tctgttgatc gacacctacg acacaattgc aggcgcgcgc
     2281 aaggccgtcg aagtcgccga ggagatggct gcccgtggtc agcgactgag aggtgtccgt
     2341 attgatagtg gtgatcttgc cggattggcg atcaaggtac gaaaaatttt ggacgaagct
     2401 ggattttcgg acgttgcaat tatcggaagc ggcggcttag atgaatacga tctgagcgag
     2461 ttctcaagag cgcgaattcc gttcgacagc tacggcgtgg ggacgaagat ggggacctcg
     2521 agcgatgcgc cgtggtcaga catggcctac aaactcgttg aatatggcga taagccggtt
     2581 ttgaaaagaa gccctggtaa atcttcttgg cccggcgcta aacaattgtt tcggcaaaga
     2641 aacaatcaag gacagcttga gagggatatc gtcgggttac gccatgaagg tatcgctgac
     2701 accgaagcgc tgctgagaaa ggtgatggaa ggcggcaaga tagttggaac cctccctaca
     2761 ttagcccaga gccgagcagg tttagccgaa gagcttgggc gccttgcgga tctgacaaaa
     2821 gcaattcgcg atccagttct ttatcccatc gagtttagtc cagaactcgt tcgcctgcgc
     2881 gaagagacgg agcgcaagct aatcaagcct tagccacctc gatcttctac tagtcggaga
     2941 acatcagaac tgttcggaaa ggtcagtccc gatctaaatc atgatgtcaa taatcgtgtg
     3001 ccggtgaaga cggattccgc tttattgcta ccggcgagaa tcgttggaag atgttcgaca
     3061 gaatcaaaac tttaccacgg cagcacccca ggcaaatccg gcaccgaacg cattgagcaa
     3121 aacgatattg tccttctgaa tacggccctg gcgcttggct tcatccaaag ctacgggaat
     3181 cgaggctgca gacgtattgc cgtatttttc gagattgacc atgactttat ccattgagac
     3241 tcccagcctc tcggctaaag ctacgatgat gcgccgattg gcctgatgtg gaatcactaa
     3301 tgacacttgg tcgatgccga caccagcttc ttccagtgcc tcacggctga tgtcttccat
     3361 ggaacgcacc gcgatcttga atacctcttt cccattcata gtaatcgtat gctcgtttcg
     3421 ttccacggtt ttgcaattgg cgggttttag agagcctccc gctgggacat aaagagtttt
     3481 tgcatacgag ccatcggtgc gcagtttcgt gcttaaaata ccgttgccgt tgtcagacgc
     3541 acccaatact acggcacccg ccccatcgcc gaacaatatg caggttgtgc gatcttgcca
     3601 attcagtaac cgactgagag catcagaacc cactacaaga gcattgcgaa tcttgccgag
     3661 ccgaataaaa gaatccgcta ccgataaggc gttgagaaat cccgagcagg cggcgccgac
     3721 atcgagtgaa aaaactcgtc tagctcccag catatcttca agcacgcaag ccgaacttgg
     3781 aaacggataa tccgcagtga ccgttcccat aattatcgcg tccaaatcat cggggttcaa
     3841 acccgcgtcc tcgagggctc tggttgcagc atggaaggcc atatccgcgt tgcccttacc
     3901 ctcttcaagt attcggcgct cctttatccc agtgcggacg gtgatccatt cgtcgctggt
     3961 ctcgaccatc ttctcaagat cgcggtttgt aatgactttg gacggtaatt cagatccggt
     4021 tccgagaatc acactgcgcc gctgtcctcc actcattccg ccctcctcat tatcacacac
     4081 gcattcgtcc cgccgaaccc gaaggaattc gaaagcacgg tctttacatc cgccttacgg
     4141 gcctggtttg gcacgtaatc cagatcgcat tgcggatcgg gattatcata gttgatcgtc
     4201 ggcggaatca gaccgtgata aagggtaagc gctgagaata cgccttcaac agcgcccgcc
     4261 gcaccgagta aatgaccggt catagatttc gtagaactca cggctagctt ggctgcctgt
     4321 tcgccgaaca cccttttgat cgccatggtt tcgttggcgt cgttatactc cgtggaggtt
     4381 ccatgtgcgt tgatgtagtc cacgtcagtg ggcgcgagcc cggcatcttt taatgccagt
     4441 ctcatacagc gagccgcgcc ttcgccctcc ggggccggcg cagtcatatg gtgcgcatcg
     4501 ccgtttgccg catagccaac cacttccgca taaattttcg cgcctcgttc gagagctctc
     4561 tctctttctt cgagaatgag aacaccggag ccttcggcaa tcacgaagcc atccctatct
     4621 cggtcgaagg gacggctcgc gcgttccggc tcattattgc gggtggacag cgccttcatc
     4681 gcggcaaagc cgccaacggc cagcggagtg atagccgact cagcaccccc ggtaatcacg
     4741 gcgtcctgca agccgcgccg gatcaagtga taagcctcgc cgattgcgtg tgtacctgaa
     4801 gcgcatgcgg atgttggtgt taaattgatt cccttggctc cgtggcgaat tgcgatctga
     4861 cccggagcca gattagatat tactttcgga atgaaaaaag gagatatttt ccgcgggccg
     4921 ccctgcagta tggcctcctt ggtactttcg atggtatcga tgccacaaag gccaacacca
     4981 atgatgacac ccacctgctc agccagctcg gcatcaatct tcaagccgct atccatcatc
     5041 gccatcgctg ccgccgccac cgcatactgg atgaaaagat ccatcttctt gatctcttta
     5101 ggctcgatga aatctgcagg agaaaagtca ggcacttccc cggcaatttg tgacggataa
     5161 gcgtcaacat tggcaaatcg gctgatccgt ctgatcccgg atcgcccggt gatcagagca
     5221 tcccaattct tttcaacgcc agttcccagt ggggtgacca aacctatgcc tgttaagacc
     5281 actctgcgtt gtttagctgt attcacgttg tttaccgatc gaaaaaattt aaaccttaac
     5341 ttgaaccatt aaaatagatg agtccgaggt gagggtcaat acttcagtga aattgatcgt
     5401 gcgttcgcaa cagtgcacga atgagaggat ctagtttgtc agcccttaca tcggacggaa
     5461 acttcccgtc gctggagtgt tgaaggactt cgagaacacc ggcaaccgag gatcttagag
     5521 cgatcagatc atagccgcct tcgagcaaaa atgcgatcct gccatcgcaa taaaccgctg
     5581 ccagttgcag catggcgcgc gccatctgcg agaatccagc ttccgttaca tccattcccg
     5641 ccaatgggtc ctggcgatgc ggatcgaacc cagcggatac taaaatccat tgcggtcgga
     5701 aacgtttgat acccggaatg acgatgtctc tgaaaactcg caggtattcc gcgtcaccgc
     5761 agccggcggg cagtggaacg ttaatagtaa aacccgcgcc ctgtctgagt ccgacttctg
     5821 caactccgcc ggtacccggg tagaaaggat attgatgggt cgagataaac aataccgaag
     5881 gatcatcgta gaatgcgtct tgggtgccat tgccgtggtg cacgtcccaa tcaacgatgg
     5941 caatcctctc tgcctgatgg gcacttttca gatactgcgc gccgacagca atggtgttaa
     6001 aaagacaaaa acccatagct ctgcttctga gcgcatggtg tcctgggggc cgcaccagag
     6061 caaacccgtt ggacgattcg ccggcagcaa cggagtccag caatcgcaga aagcctccgg
     6121 tggcatgcaa agcgacagca aaagaatcct gactagtaat agtgtcaccg tccaaagcgt
     6181 actggctcag gctggcggtc gattccacga gcttgatata tttcggatca tgacaaagct
     6241 ctatttccga gatagaggcc gctctgcccg gcaatagctg aaatcgagca ggatcaaggc
     6301 tctcggccag gtccagcaaa acccgcagcc tctccggtcg ttcaggatga ccgcgacctg
     6361 gcatgtgctt gagaaactct cgatcgatca aaatggcgga gcgcctcatt agagtaaact
     6421 agcattcgcc gtcaccgctc acaacgttgc tgacccgcac taattctcca tcccattgcc
     6481 gggggcagtt gttcggacat aggcccgaat ggtaccggca actcgttggc acgacattta
     6541 gctcagttgc caactcatac aaaatcgcat agcctgacgg tggaggatat agagatgttt
     6601 ggcattggga tgccggagct attattgatt ttagctttgg cgctgatcgt tctaggtcct
     6661 aagaaactgc ctgagcttgc ccgcgcgctc ggcaagggaa tggcagagtt tcgaaaggcg
     6721 accgatgacc taaaggacga gttccgtcaa ttggaggagg agccatctga atcgtggact
     6781 cagaccgcta aaaaggacga ccccccgcta accaagaaag ctgaagtgtc ggcggcgtcc
     6841 cctgcgcctg ctaacgaacc acgtgagtaa tggttcagcc aagagacatt tggacaacgt
     6901 ccagatgcct cttgcctccc atctagatga gctccgctca agactgatcc gctccgtcct
     6961 tgctgtcgct atcgctttcg tcctctgctt ttatttcgcc gacgaaattt tttctgctct
     7021 agcagaacca ctccggagcc ttcctatacc cgatttgatt ttaatcggta ccgcggtgac
     7081 cgaagccttc ttcacaaaga tgaagatctc gtttttcgcc gcgctgatcg tcgcgttgcc
     7141 ggtgctgttg tggcaaggct ggcaattcgt cggaccggga ttatacgaac atgagaagcg
     7201 ttatacccgc agcttcgttc tttttggctc gttttttttt ctcgcaggcg tggctttttg
     7261 ttatggcatc gttttaaaga agggcctggc gtttatgcta aagcgatatc aggtgctgca
     7321 gatccagccg gcgattcaga tcggtgacta tctttcgatg gttgccaggc tagtcttggc
     7381 atttggagtg atgtttcagc tgccggtgct tacttttttt cttgctcgtg tagggttggt
     7441 ggatcatcgt ttgttgattc actatcttgg ctacgcagtc atcggcatgg cgatcatctc
     7501 ggcggtgcta accccgcccg atctgatttc gcagatctta ttgatggtgc ccttaatctt
     7561 gctttacggg gtgagcattg ctgtagcata cgccgcgaga ccgcggaaaa tgcagtaaac
     7621 gtatgcgatc ttaatcataa aagcgccgac tgcggcgagt tatgggcagg aaaatcaatt
     7681 gtcgtcgtta acgaaagcgc aggcggcgaa gactgtagtc caaagcccat ctttattgcc
     7741 gatggccgat tgcgttatat tggaagttcg aacgattttg ccggacattt taaacaggtt
     7801 ttccctctca tcccaagcgg tgtcaggatc aaactcaatc cccagcgttg tagccagcat
     7861 actggccgct aaatcttcgg cgtaatcgcc ggctttttct tctgtttctc cgaacgagtg
     7921 gtgctccgaa aggtaaccat gctgctcttg gtctgccgga atagcgacac cgaccgacgc
     7981 cgcgaccagg cggttcggct cgtttgtact ctcacgatcg tagacgcaga aaacgatctc
     8041 tccggggcgc aacagcttga cgccttcctc tttggaaatg cgtttgcaaa aaggtggata
     8101 gatgcttgag accagaacca agttgcagta ggccagaccg gctgatcgca aggccaactc
     8161 gaatgatgcc aacttttcct tatggacgcc aacacccttg gtaaagaagc acttttttgg
     8221 aatcattgac gtgtgtctat aacattaaag aaatttgctg acaacttcga aaactaaatt
     8281 gtttcagttc tttgttattc tacgatcagc aaaactgcgc cgccctccac ggtttcgccg
     8341 gacttcactt tgatctcttt tacctcgcca ctgatcgggc tgcggacctc gttctccatt
     8401 ttcatggctt cgacgatcac caggccctgc cctttttcga cggtatctcc ttcggccacc
     8461 agcacggcga tcaccttgcc gggcatcggc accgaaactc tctggcgtcc ttggagctca
     8521 attccggatt gcgtaccgcc gacacgaacc cgccgctcgt ccagcaaatt gacgtgatag
     8581 ttgcgcccgt ccactaggac ttgatattcg tcttccatgt gatcgacttc gatctcgaac
     8641 gatcgattgt cgacgatcaa cgaataatta gtccgcccgg tttttttccc atcgatcaag
     8701 aattcattgc catcgatcga aaccttataa accgacttgc cgacttcatc gatatcgacc
     8761 gtgtaggtct gctcccccag cttcgcaatg aacgccataa tggtctggct acgaccggcg
     8821 ccaaccccgt gcgtggaatg ggggctgtag acgcagcgca ttgcgcttac cgcgctcgcg
     8881 ccatttcgat ggttcggcag cgcctctggc tagcagccca agcgtgcttt cctgttcgcg
     8941 atgcatggcg gcgatggctg ccgatgccaa cgcaatctct ttatgcggaa agacctcctc
     9001 tttgctggtc gaatgatact cctggtcgat aaatccggta tggactttgc ccgacatgaa
     9061 atttggatgg cgcagaatcc attgatgaaa ggatatgttc gttttgatgc cccggacttg
     9121 atactcacgc agcgcccggc gcatgcgcag aatggcctcc acccggttct caccccaggt
     9181 aatcagcttt gcgatcatcg gatcataata aatcggcacc tcggcgcctt cgtaaacgcc
     9241 acagtcattc ctcaagccaa gtccctccgg taagcgcaag ccttcgattt ttccgggaca
     9301 cggcatgaaa tcgttttccg cgtcctctgc atagatccgg cactcgattg cgtggcccgt
     9361 ttgggtgatg tgacgctgtc gccaaggaag gtatcccccc gcggctatct caatctgtgc
     9421 cttgaccaga tcgatcccga tcactcgttc cgtcacagca tgctctacct gtaaccgggt
     9481 gttcatctca agaaaataga aattctgctc ctgatccagc aggaactcga gcgtgccggc
     9541 gccaacgtat cgaaccgctc gcgctccttg gatagcaatc cgcccgatgc gccgcctagt
     9601 gcgatcgtcc aatcccggag cgggagattc ttcaatcact ttttgatggc gtcgctgaat
     9661 cgagcattcg cgatcgaaga ggtgcaggac ccggccatag ttatctccga ggatttgaat
     9721 ttcgatgtgc cgtggtttct cgatgtactt ttcgacatag agtcgcgggt cgccaaaaga
     9781 tgatgccgcc tccgatccga ccgctcgata ggccgatgag acttcgcgtc gagaacgaac
     9841 cagacgcaaa cccttacctc cgccgccggc caccgccttc aacatacacg gatacccaat
     9901 ggcctctagg gtacttaaga cttgctcttc ggaagtcagg atgccgtcag agcccggaac
     9961 gaccggcact cctgccgcct tcatgatcgc tcgcgccttc actttatcgc ccatctgatc
    10021 aatgacttct ggagacgggc cgatgaagac aattccctcg gccaggcagc gctttgcaaa
    10081 ctggctgttc tccgccaaaa aaccgtagcc cggatgcatt gcctccgcat ggcttctttt
    10141 tgccacctcg atgatgcggt cgatattcaa atagctctgc actgaaggag ccggaccgat
    10201 agcgtatgca tagtcggcat actttacgtg caaggcctcg cgatcggcct ctgaataaac
    10261 cgcaacggtc tcgatatcga gttcccgaca ggctcgggca attcgcaccg cgatttcacc
    10321 gcggttggct atcaagatgc gtttaaacac gtgtgacttg attcatcagc caacggtcaa
    10381 cggggccagc gtaacaatct agagaggaat attcccatgc tttttgggcg ggttatagtc
    10441 ttgtttattt ttcaacatct cgagtgcgcg aatcagcgtg gggcgggtat cctcgggata
    10501 aatgattgaa tcgatgtagc cgaattctgc agccttaaac ggattggcaa acgtgttgcg
    10561 atactcctga gtcatttcat cccgcgcttt gcctgaatcc tcagcctgtt gaagcgcttc
    10621 cctgaaaaca atgttgatcg cgccttccgg acccatgacg gcgatctcag cagtcggata
    10681 agctaaatta atgtcgccag cgagatgctt gctggacatg acgatataac cgccgccgta
    10741 tgctttgcgc gtgacgatgg taatcttggg aacggtggct tcagcatagg cgtaaagcag
    10801 tttcgcgccg tgacggatga tgccgtcata ttcttgcgaa gtgcccggca aaaatcccgg
    10861 cacgtcaacg aaagttatga cgggaatatt gaaacaatcg caaaatcgga tgaagcgcgc
    10921 gcctttgagc gaagcattaa tgtccaagca tccagccagg gctgccggct ggttggctac
    10981 gataccgaca ggacgcccgc ctaaacgagc aaacccgatg agtagattcg gcgcgaaatc
    11041 ccgctgcact tcgtaaaaat agccctcgtc gatgacggcg ccgataagat ctttcatatc
    11101 ataggggcga ttgggattgt ccggaaccaa atcgcgcagc ttcttgtcgc gccgcaatgg
    11161 atcatcattg gtgggatgaa agggaggatc ttcttgattg ttactgggca ggaatgcgat
    11221 cagctcacgg atcatcagta agcattcttt ttcaccattc gcggtaaaat gagctacgcc
    11281 actcttggcg ctgtgggttt cagcgccgcc caattcatcc ttagtgactt cttcgtgagt
    11341 gaccgtcttg ataacatcag gccccgtgat gaacatgtag ctgttggtct ttaccatata
    11401 gacgaagtcg gtaatcgccg gagaataaac cgctcctccg gcgcaaggac ccatgatggc
    11461 tgaaatttgc gggatcaccc cggatgcttt cacgttgcgc aagaaaattt caccgtaacc
    11521 cgcaaggcta agcacgcctt cttggatgcg ggcgccgccg gaatcgttga taccgataat
    11581 gggcgcccca tttttcatcg ccaggtccat aatcttgcaa attttttcgg cgacgactcc
    11641 gctgagactg ccgccaaaaa cggtaaagtc ctgtgagtat acgtagacca ggcgaccatc
    11701 gatggcgcca taaccggtga ctacggcatc accgaggaac ttctgttgct gcatttgaaa
    11761 atcagtgcag ccatgggtgc gcaaacgatc taactcgaca aaactaccgc tgtcgagaag
    11821 aacgttgagg cgctcacgcg caagaagttt tccttgttca tgttgccgcc gggcccgtag
    11881 ctcccctccg ccagcctcgg ccttctgatt caactcacga agtctctcga aattgttgtt
    11941 gatggtcatg aagaaacgtt ccttagccgc gcaagtcagc gggaggcggg gatcacgagt
    12001 tgcagcatca taacaaaagc gcctagtgtg tcaacagaat ttgccgtcgt agcattatct
    12061 ttattgaagt tacatcgcga agttgttagc ctgtcttgct tccagataaa atacgccgag
    12121 actgaaagaa tgctgcaatt cagatccaac cgaaccaacc gtgagcacgc cgcatctttg
    12181 aacacggctg atcgatcttt gaagccggat gatgcgttgc ttctagtcgc gtccagcgaa
    12241 ggcgactcca acatgctcta tgctgttggt ttctttgtcc ctgatccgtt catttttttt
    12301 cagcataaga aaaagaagta cgtggtgatg agcgatctcg aggttgaccg ggcaaagcga
    12361 caagctaccg tcgatcaggt gctttcgctc tccgactatc agcgaaagct gagaaaggcc
    12421 gacagccctc caacaatgat cggtattctt gaactgctgt ttcgcgagcg cggtatacgg
    12481 tcactcaagg taccggcaaa tttttcagcg cttctggcgg atcagctgcg agataaaggc
    12541 ttcaacctcg agattacgcc tgatccgttt tttcctgagc gtgaaatcaa acgcaaccag
    12601 gaagtgcggc acataaccga atcgctgcac gtggcgaaat tggggctcga tgccggcatt
    12661 aggatgctca agaaaaccaa aaccggacac gacggctatc tatatttaaa cggctcgaag
    12721 ctcacatccg aaacactgaa aaccgctgtg aacacgacga tcatggcgca agggtggctg
    12781 cccagccaca cgatcatttc gtccggtaat cagtgcgtcg atcctcatca tgaaggaacc
    12841 ggcccgatca aggcgaacac ctcgattatc ttcgatatct tcccccgctc gcagaaaacc
    12901 ggttacttcg gagatctcag ccggacggtg gtgcgcggcc gggcatcgga caagctcaag
    12961 aagatttatg cgactgtgca ggccggacaa aagatcggct ttgacctgat tcgcgatggc
    13021 gtcaatggca aagaggttca ccaaaagata ctcgatctct ttgcccgtga aggttttccc
    13081 accggacaaa tcaaaggccg catgcaggga ttcttccatg gcacaggaca cgggcttggt
    13141 ctggatatcc atgaggcgcc gcgaatcgcg ccggtcgatg ccacgctcaa gagcgggcat
    13201 gtagtgacgg tagaaccggg actttattac ctcggagttg gcggcgtacg attggaagat
    13261 gtagtgctgg tcaccgataa aggcaataaa aatctcaccg actgtcccca gtttctggaa
    13321 atctaatcgt gacccgtgca ggtctctcac aatctgggcg ggaacttcgc gctctataat
    13381 ctggaaacta ttgtgcaaca aacaaagatt tccgaatggc tggaggcgct cgaccggccg
    13441 aacaagcaga tgctccgcgc cgcggtggaa ggcttgatcg cggctttgcc ggactcacct
    13501 gaaaccggca ggaatctgct gctgctgctg cgcgatccag agcgaaaaaa tcgctggccg
    13561 atcgcctaca ttctcgccca cttgcctcag ccgcccgacg actcattgga aatactgctc
    13621 gatgcattag accatcccga cccggacgtg cgctgggccg ttatcttact gttggcgcgg
    13681 ttgacgaaag aacaagagtc gatagtgcaa cttacgacgt ccgtgattgc caccggcacg
    13741 gcgacccaac ggcgcatggt aatttattac ttgaaagaat tgaaacgtcg gagcgaagct
    13801 gttcgacagg cgctattagc cgcgctgaac gatcctgatc cgacggttag agtcgccgca
    13861 gtgatcagct tgaaagatca ggatgtagat tcaaacggta aaacaactct actgaatttg
    13921 ctgatgcgcg atgcggatgt tcgggtacag cacgccgcag cgatcacgct ggcaagcttg
    13981 ggatcaccgt ccgaggaatt tctatcggct ttgaatcacg ccactcaatc cgcccaggag
    14041 ccgttgaaga aggctgctct agcggcacag cgcattcttc aagaaaaaaa agaggtccgt
    14101 cccatccggc aactaacgtc accggtgaga cggacctcga gttaacttcg gtagactgag
    14161 ggggattgct aggcttcaag ctagaatccc cgtcaaagat ccacttgaag cctaagtctc
    14221 agtcacctcg cagacaaaac aattatcatc aatcatgtct ggaccacctc ctttccctgc
    14281 tgactttcag tttaaggagc aaggtgtctg ctgtcaacag ggatctagcg cagctacgat
    14341 ttttttagac gcgaatgaat tttttagcag cgatttcgac atgaaattta ttcagatcgc
    14401 cagccgataa aactgatctg acggccggtt tctttttttt ctcgtcgata cgagaaaaat
    14461 tcatccttgg cgcagctggt acacggaccg actcgaaaga tttgactttg cgggataccg
    14521 gcgcggtgca gaatggcgcg attcagattg cgcaaatcaa gatagctctt gttgtctctg
    14581 ttctcgatag aagaagttgt cagctcagcc cattgttcga tcagcggctg cgctacgtcc
    14641 cgaccgatct catagcagca cgcgtcaatt gatgggccaa gggcaacttc cagctcggaa
    14701 ggcaacacac tataactttc caagaacaat ctgaccgtct tgccggcaat tcctttcagg
    14761 gttccgcgcc agccggcgtg tacgaccgcg acaatcttct gctgccgagc tagcaaaagg
    14821 atgggcacgc aatccgctgt taacacccca agaaaggtat tccgcgcgcc cgtcaccatg
    14881 ccgtcggcct caccggcttc cttgacgctg gtccctttga cctcgatcag gtgatcgccg
    14941 tgaacctgct tcatagtaac gatgcggcca tcatgaatgc cgacggcgag cttcatatca
    15001 cagacgttct gactgaccgt tttaggatcg tcaccgacgc ggtacgaaac attaagcccg
    15061 gcataagctc cgacgctttg gccaccctgc cgccccataa aaccgtgcag taaatccgga
    15121 tcattttccc attgcgggac tttcaaccaa ttgattttca tacccggtat tatgtttgga
    15181 tcattggcgg accgcaagtc ggtcgagaaa ctccggtcgg ttgttacgct tgcattgtgg
    15241 ccgaatttca ccgaaaaata cccgataatt cttcggcaat cctccatgac aaacggaaga
    15301 tacatcggca cgctcaaaca agttggtttc ttcgcttttt tttgcactca gtttctcggc
    15361 gccttcaacg acaacttcta caaaattgtc gtatccctgg tggcgcttaa tgccgcaacc
    15421 cccgaaagcg atagcttata cgttgacttg atcgcgttcc tctttatcct gccgtccgcg
    15481 cttttctccg gatacgccgg tcatttagcg gatgcgtaca gtaagcgctc gattcttgtc
    15541 gcggtaaagt gcttcgaaat cgtcatcatg gggatgggat ttgccgcttt tttgtccggc
    15601 tatgtcgagc ccatgctggt catcgttttt ctcatgggac ttcattcagc gttcttcagc
    15661 ccggcgaagt acggcattct ccccgagatg ctgcccgaga ctgaactctc gcgcggtaat
    15721 ggcttattgg agatgagcac cttcatggcg atcatcttag gggtttctct tggcggggca
    15781 atctattcga tctggaagga ccagcttcca ttggttggtc tcttcatggt cgcgatcgcg
    15841 gtgctcggaa cactaaccag tttgaagata tctcccgtgc cggcttcggg ctcgaccagg
    15901 catttcaatc tgaatccgtt cggtgaaatc tggcatggca tcaagcggct ttatcccgat
    15961 aagccgcttc gcctgactgt gattgggatt tcctattttt ggtttctcgg agctttggtc
    16021 caactcgcaa tgttgttttt cggcaaagag ttgctcgggc tcgatgaact gcacatcagc
    16081 ctgctgggaa ctttcctcgc cattggcatt ggcctcggca gtttggcagc cggaaagtta
    16141 tcgggtgata agattgagct cggactggtg ccgctgggat cgatttcgat gggagtttgt
    16201 ttattgctgg tctcgtattc cggatcatcg tattttaaga cttcggcgtt tctcgtgctg
    16261 ctgggctttt ccggcggcct cttcgccgtc ccgctcaacg cgctactaca acatcgcagc
    16321 ggtcgcgagg agaagggcca actcatcgcg accaacaact ttttcaatac aattggcatc
    16381 atcttggccg cgcttgttca ctggattttg aatagcgcgc ttcaactatc gcccgattgg
    16441 atcattttca tcatcggagt actcacgctc atcgctacgg ccattgtttt agtggttctt
    16501 ccggactatt tcgtgcgcct cgtcgcctgg ttgttgacgc acacacttta tcgaatacgg
    16561 atcgcgggcg aagaaaacgc tcccgtacac agtccggcgc tgctggtgtc aaatcatgta
    16621 tcgtttgccg atgccctgct gatcggcgcg tgtttgcctg gtttcgttcg tttcatgctg
    16681 catcgcgact attatgaaaa caaatggctc aactggtttt ttcgtctgat gaagtcgatt
    16741 ccgatcaggg caacccatcg ccgcgatgtc gtcaattcgc ttaaacgagc tcgccaggag
    16801 ctcgaggcgg gacacgtggt ttgcattttt gccgaaggcg ccatcacccg caccggccac
    16861 attcttccct tcaagcgcgg cttcgaaaaa gttgtcgagg ggacgagtgt tccgatcatc
    16921 ccggttcact tggaccaagt ctggggcagt gtttttagtt ttcatggcgg gcggttcttc
    16981 tggaaatggc cgaagttctt tccctatccg gtcactgttt cattcggtaa accgctgccc
    17041 gccaatgcta cggcaacgga ggtccgtcaa gcagtcctgg aactcgccag cgaagcggca
    17101 cggtatcgcc gcaaaccgac cgatttatta cccgctaaat tcgtccagag ggctaagcgc
    17161 cggtggtttt ccttctgtat ggccgacacc accgggaccg aacttacctt cggcaaggct
    17221 ttgatcggga gcatgttgct ctgccgctgg gtaagtgagc attcgccgaa agagcggatg
    17281 gtgggagtca tgcttccggc ttccgtcgcc ggcgcgctgg caaacctggc gatcatgctg
    17341 gcaggaaagg tgcccgtcaa tatcaatttc actgccggac aagatgcgac gttatcagcc
    17401 atcaaacagt gtggcatcaa aacaatcctc acctcgagat tatttctgaa caaggcaggc
    17461 ctgcaagaat tgcctgggat ggtttttctg gaagaagtta gaaagacatt caccccggcg
    17521 ctaaaacttg ttaccgccgc caaagcagcg gtattgccgg cggtttattt gaatcggaac
    17581 gtaacgaaca gcgcggcccc tgatgatctc gctacagtga tattctccag cggcagcacc
    17641 gggatgccta agggagtgat gctctcccat tcgaacatca tctccaacat cgaaggtatt
    17701 tgccaggtga ttcaatttac gaaaaaagac cgcatcatgg gcatactgcc attatttcat
    17761 tcgtttggtt tcacggcgac attttggctg ccgctcctgg caggcttcgg cgtggtttat
    17821 caccctaacc cgatggacgc caagaccata ggggaaaccg tccagaagca ccaagcgacc
    17881 ctgctgatca gcaccccgac cttttatgcg ggttacttaa gacgctgcag ccccgaggaa
    17941 tttgccagtc tgcgctatgt tattgccggc gcggagaaac tgcgcgagca gattgccaga
    18001 gcctataaag agaagtatgg aattgatctg ctcgaaggct acggctgcac agaactcgcg
    18061 ccggtagtct cggttaatat tccggacgtg atcgacaatg acgaaaaaca gatcggtcac
    18121 aagccgggca gcgtcggtca tccgattcca ggggtcgcgg tcaaggtgat cgatcctgaa
    18181 acggaacggc ccttagactg cggccaagaa ggtttgcttc tcgttaaagg cccaaactgt
    18241 atgctcggct atctcgggca gcctgagcac acggcgcagg tattccggaa taaatggtat
    18301 gtaaccggcg acatagcgac aatcgacgag gacggcttca tacgaatcac cgatcgcatg
    18361 agtcgtttca gcaagatcgg cggtgaaatg gtcccgcaca tgaggataga agaagttatc
    18421 aatgagattc ttggtagtgc cgcagctgtg gttacagcgc tcccggatga gcaacgtggt
    18481 gagaagctga tcgcttttta cacgcataat ggcattagca gagacgaact ctggaacagg
    18541 ctcaaccaat caacactacc aaaactttgg ataccgaaaa gagaggatct cttcatcctt
    18601 gaatccatac cactgctcgg ctcggggaaa atcgatttga aacaaatcag a
//
LOCUS       DASUVS010000063        46061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_241277, whole genome shotgun sequence.
ACCESSION   DASUVS010000063 DASUVS010000000
VERSION     DASUVS010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 46061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46061
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241277"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..505)
                     /locus_tag="VF182_03615"
     CDS             complement(71..505)
                     /locus_tag="VF182_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX6436191.1"
                     /translation="MDALELLKQDHARVKKLFDRTETADDREQKRIFLQIKTELEIHA
                     QIEENIFYPAMQRHDELKELVAASLKEHHAMKALLQEMATLSAREDFEDKLEELIDNV
                     EHHAEDEEEGKIFPKVRELVSANELQKLGAQLQAAKGHRKAS"
     gene            complement(506..796)
                     /locus_tag="VF182_03620"
     CDS             complement(506..796)
                     /locus_tag="VF182_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436192.1"
                     /translation="MQSAHAPAEEIMKDDIADKPHVTDAQLSLLEQHYELKPRLEFRR
                     EVSRGKPLTVGPAARLSKGVTWQGLAQMSTTRFANGSVNVKTANSIRAVQGG"
     gene            1611..1856
                     /locus_tag="VF182_03625"
     CDS             1611..1856
                     /locus_tag="VF182_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436193.1"
                     /translation="MGKTMEALYNGNVIRVSALRVPHKHQWTFTWVVWKPGTEAGVKT
                     FTDYAGRFDTADDAISRGYDFAMKWIDDGKPDLLEER"
     gene            2120..3208
                     /locus_tag="VF182_03630"
     CDS             2120..3208
                     /locus_tag="VF182_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6436194.1"
                     /translation="MTIFNGELGLQFVTHLANQYTVPHLVRLAGLAADKGFQQIWVND
                     NIRYRSQLVVLTAIASQVPVRLGTAIMVPYFHNPLDVADSLGALSELCEGQEISVGIA
                     RGDLGQSPQHVQPLRPIAMVSETVQLLRQALSGEELSYADYPFLQEFYHLNPRGKFRL
                     AFKPRSSFKFYGGGNGSQSLRMCGRIMDGLISSGTYIPMLKAGRLPGMLATADRAAQE
                     VDRGKRLRKVCELNVSISRDRAKAIEFPKRQVAHSILQWEALGFTAEEYDKMGVSREQ
                     VLSLKRAFEAGATVEEAAKLVTEHMVKTYYAAGRPEEVRDQIIELAGAAGKLGYDHIA
                     FAKLGPDYEEAINLLADQVVPALTGRGL"
     gene            3412..4404
                     /locus_tag="VF182_03635"
     CDS             3412..4404
                     /locus_tag="VF182_03635"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436195.1"
                     /translation="MRKTSFATTLCLSLALIIGTSLQAESKLLSAYISDSPSSSVPAW
                     IAKDAGLFKKYGLDVDLVFIDGSTRGIQSLLAGDLSFTEAVGTSAINGRIAGGDIAIV
                     NGVVNTLPYYIVGKGNIKSREDLKGRTAAVHIPGTAADFALRLALKQVGIPYNQIKAI
                     TVGGGPARIASVIAGQTDFTIVSDAEKIQGERNGLKVVIDMAALKVPFLYTCTVTSRQ
                     MIREQPNAVQRMVEAIAAGVQFFKANKEESIKIMNKYTRGANREILEGAYSAYSQLFV
                     EDTYPTLEGLRNTLEVQSSWDPKAGKAKAEDFVDLRFVDRLRSSGFIDKLYGRK"
     gene            4482..5318
                     /locus_tag="VF182_03640"
     CDS             4482..5318
                     /locus_tag="VF182_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6436196.1"
                     /translation="MLRTYNNVSVPSFMYGTAWKKEATARCVLLAVESGFNAIDTANQ
                     LIHYQEALVGEALKSLEQQGAKRDSLFLQTKFTPAGGQDHRTPYDPSADLTTQVNQSL
                     ASSLNHLATDYIDSYVLHAPFSRRGLGEADWEVWSAMEALYESRKTKMIGVSNIAAGQ
                     LIELCRRANHKPMVVQNRCFAALAWDKQIREICQAEDIIYQGFSLLTANREVLVDSQV
                     QKIARSQGATSAQIIFRFAIQVGMLPLTGTTSQQHMREDLEAERLTLSSDEIELIETI
                     AV"
     gene            complement(5564..7594)
                     /gene="tgt"
                     /locus_tag="VF182_03645"
     CDS             complement(5564..7594)
                     /gene="tgt"
                     /locus_tag="VF182_03645"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX6436197.1"
                     /translation="MKGELNFMLEKQSSGSKARAAKFTTLHGDVQTPIFMPVGTQATV
                     KGQTVESLTAAGARVLLVNTYHLLLRPGMDVLKSFGGIHNFMNWHGPVLSDSGGYQIF
                     SLPQSREINEEGARFQSYVDGQSHLLSPESSIEMQKAIGSDIMMALDVCIPATAPYAQ
                     ARWAMEITHGWAQRSLRARGDSRQALFGIVQGACHADLRRISAEFSSGLAVDGLAIGG
                     LAVGESHHERYDVTELVAGHLPENLPRYLMGVGTPIDILESVHRGVDMFDCIVPSQLA
                     RRGVAFTSHGKLQLRRSVHKFSQEPLDTRCDCRSCRQHSRGYIHHLIKCDELLGWHLL
                     GIHNLTFYQRLMSEMRQSILADVFLAYYETKRSELTRSDEDNPVRRPRSAKASQSTRM
                     GDYEIRTFAEGFSSIRQISSGEIMHSVNAPDAEAHRLYVEQSCLAARLMKQESEINEL
                     VIWDVGLGAAFNAMAVIHCFETSLAKQGAQALRRLRLVSFEWDFDPLRLALTNPSRFP
                     HLRHGAPRRVLESAKWEHTSNLLEWELLEGNFADVMESAKAPKLIFYDPFSYKTNAAL
                     WTVETFSRIFDHCRGEPAELYTYSAATAVRVALLAAGFFVAQGIGTGPKSDTTIAFTT
                     VSGSKDHPLSPRLLGPSWLTRWRRSDAKFATTVRDEDKAAFEKLIESHRQFT"
     gene            complement(7686..7946)
                     /locus_tag="VF182_03650"
     CDS             complement(7686..7946)
                     /locus_tag="VF182_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436198.1"
                     /translation="MDGEELETANWGRSSAGDALRFRSASGVGCEFAWPIIMRGDTKL
                     CAPFNDSLNPADQACAGGENMPNVSFEDAGSCHHEPLERPAQ"
     gene            complement(7928..9550)
                     /locus_tag="VF182_03655"
     CDS             complement(7928..9550)
                     /locus_tag="VF182_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEX6436199.1"
                     /translation="MELATEQTGAARLSEEVARRRTFAIISHPDAGKTTLTEKLLLYG
                     GAVETAGAVRAGRNQRHATSDWMDLEQERGISITASALEIEYQGFRLNLLDTPGHQDF
                     SEDTYRTLMVADSAVMVLDSAKGIEAQTEKLFQVCRMRGIPILTFINKLDHFGRDPLE
                     LMDEIERVLGIATAPMNWPIGMGSTFQGVYDLQHRRVLQFERTERGQWRAPVAVRNLD
                     DPALSALLGEMLGSELRDHIALVTGAGNAFDRRRFLAGELTPVFFGSALNNFGVEPFL
                     EALLNLAPEPSAFEKNGTLLQPTADCFTGFVFKIQANMDPRHRDRMAFLRILSGRFEK
                     DMTAYHPRLEREVRLARAYRIFAREREPVREAFAGDIIGVINPGLFVIGDTVTTGEPA
                     ELIDIPRFPPEHFAVLINQEIVRYKQFHKGLSQLEEEGAVQVMHAAGGARREPILAAV
                     GALQFEVVLSRLRQEYGVEARIERLPFTCARWIKSQSVERMTLGSGVLQCRDQNGDTV
                     VLFASDWHLQYFRKENPQIEVVNVGSPWTAKN"
     gene            complement(9774..10610)
                     /locus_tag="VF182_03660"
     CDS             complement(9774..10610)
                     /locus_tag="VF182_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6436200.1"
                     /translation="MRTLVHLSDLHFGTVRQEIVLPLLSLLERIQPDVVAVSGDLTQR
                     ARKEQFVAAREFLDAIPFPKIVVPGNHDVPLYNLFARFVLKLTRYRRYISDELQPFYS
                     DPEIAVLGINTARSATFKGGRVNSRQVRHMRERLGSFNGVTKIVVTHHPFDLPEAYPD
                     KALVGRARRAMQQLTGLGIDLFLAGHLHIGLSEPTALRFKISGRSPLMIQAGTALSSR
                     VRGEANSFNVIRIQHPNISVSRLSWRADLKVFVSTHADYFRHTDAGWARGSKLDFAEP
                     ED"
     gene            complement(10616..11527)
                     /locus_tag="VF182_03665"
     CDS             complement(10616..11527)
                     /locus_tag="VF182_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX6436201.1"
                     /translation="METDFIVIVNAGARKADDVAVYPQLAEMFTAKGLRAEVQLARSG
                     AEIGELARRAIKVEGSVVVAAGGDGTINAVASQLVGSNKILGILPLGTLNHLAKDLGM
                     PLDLESAVDVIAQQRLAQIDIGEVNGRYFLNNSGLGLYPGIVHEREQQQAQGRSKWLA
                     FLSALLIIIHRYPLLTVRLSTEARELRRRTPMVFVGNNEYQLQGLNMGSRRSLNGGKL
                     FLYVTREMSRWRLLKIGLAALFGRLGDTRDFDAMSVREVSVEARRRRLRVALDGEVVV
                     MRLPLRYCIHPAALRVIVPADQEESGR"
     gene            complement(11806..14673)
                     /gene="treY"
                     /locus_tag="VF182_03670"
     CDS             complement(11806..14673)
                     /gene="treY"
                     /locus_tag="VF182_03670"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016861390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX6436202.1"
                     /translation="MRIPIATYRLQFHRNFTFADAKAVVLYLRDLGISDVYASPIFRA
                     RAGSLHGYDVVDPNEINPELGGAAQFDQLAGEIQRQGMGWLQDIVPNHMAFHSENWML
                     MDVLENGLNSAYANYFDIEWDYPMENMHHKVLAPCLGKFYGQCLEDGEIVLKYDAQGL
                     AVHYFELRLPINIECYSMVLTPSLGALRRKLGRHDPDLIKLLGVLYTLKNLPSSEEAR
                     ERADQIIFVKNMLWELYTTNEEIKNFLDENIARFNGRPGAPETFNALDQLLGQQYYRL
                     SFWKVATEEINYRRFFNINELISLRVEDANAFELCHRLIFRLIRDGRITGLRIDHIDG
                     LYDPAAYLRRIRQNVGEVYVVVEKILEMRENLPDDWLVCGTTGYEFANFVNQLLCDSD
                     HEQKFSELYRRFTGHNVAFADLVSEKKRLIIGRYMAGDVDRLAHLLKRVSSRDRFGGD
                     ITLYGLKRALVEVLTFFPVYRSYISHENFGAEDRNRMQETLSKAKEVNPGLILELSFI
                     GKFLMLEKGDQLSEQEKSQWIDFIMRLQQLTGPLMAKGFEDTTLYIYNRFLSLNEVGG
                     DPSRFGLSPNEFHEFNLRRAQAWPHSLNATSTHDTKRGEDVRARLNVLSELPEDWERH
                     VFAWGEMNRPHKRLVKGREVPDRNDEYFLYQSLTGALPLAVEEHAGFIERIKNYTIKA
                     VREAKVHTGWLKPDTDYENAFLGFIERILEPSKDNAFLGELRQWTRKIARFGALNSLS
                     QTLLKLTCPGAPDLYQGTELWDLHFVDPDNRQPVDFELRDKFLADIKEREKASLKTLL
                     LDLLTHWEDGRIKLFLTYKALNFRRRQTELFQNGNYVPLAVSGAAASRVCAFARHDGA
                     IWSVIAVPRFPTGVFAAENAPLGRQGTWEDTALELPADSPAAWSNVFTGETQPAHKTP
                     NGKKYLYLRDLFSDFPVALLEGSSAASTNAAASLPLEQRLQ"
     gene            complement(14688..16847)
                     /gene="glgX"
                     /locus_tag="VF182_03675"
     CDS             complement(14688..16847)
                     /gene="glgX"
                     /locus_tag="VF182_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX6436203.1"
                     /translation="MKLWPGKPYPLGALWDGAGVNFSLFSENAERVELCLFDSAEGKE
                     SARIPLREKTAHVWHIYLPEVRLGQLYGYRVHGPYEPEQGHRFNPAKLLLDPYAMAIA
                     GTIKWGDELFGYPIGHPDADLAPDGRDSSVGLPKCVVVDPAFSWGNDVAPRTPWHKTL
                     IYELHVKGFTARHPGVPERLRGTYAGLACPAVLEYFHSLGVTAVELMPVHQFVADRQL
                     IDRGLTNYWGYNSIGFFAPDARYSSSGVLGQQVAEFKTLVKTLHREDIEVILDVVYNH
                     TAEGNELGPTLSFRGVDNASYYRLVENDRRYYMDYTGCGNTLNATDPAVLRLIMDSLR
                     YWIQEMHVDGFRFDLASALARELHAVDRLGAFFDIIHQDPVISQVKLIAEPWDLGEGG
                     YQVGNFPALWAEWNGIYRDTVRRFWKGDSGQVGSMAYRLTGSSDLYGTSDRQPHASIN
                     FITAHDGFTLHDLVSYNDKHNEANGEDNRDGHNDNSSWNCGAEGPTDDIAIIELRERQ
                     KRNFLATLLLSQGVPMLLAGDEIGRSQSGNNNGYCQDNEISWVDWNLDPRRQSLLEFT
                     RGLIQLFHDHPVLRRRHFFQGRSIRGTAVKDLSWFRCDGKEMTEEDWNDAENRCVGLI
                     LSGDAVDELDGRGNRITDDTFLFLLNAHHQRVAFMLPGPSAKAQWQVVLDTREGMARR
                     TRRVSKGANYNLEGRSLALFRLFDSHEALPMPLARRR"
     gene            complement(16844..18766)
                     /gene="treZ"
                     /locus_tag="VF182_03680"
     CDS             complement(16844..18766)
                     /gene="treZ"
                     /locus_tag="VF182_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015204977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX6436204.1"
                     /translation="MKSSDDSLHPLSFDSKADANAAIGARCLAGNRCLFLVWARNASA
                     VDLRLLGSSERIVPLVNVGDGYFRAEVENVGPGAQYFYRLNHATERPDPASRFQPEGV
                     HGPSQVVDRSFTWNDEQWFGLPLHKYLVYELHVGAYTEEGTFEAIIPHLAQLSDLGVT
                     AIELMPLAQFPGERNWGYDGAYPFAVQNSYGGPEGLKRLVDACHQRGLAVILDVVYNH
                     IGPEGNYLADYGPYFTSRYKTPWGLALNLDGAASDHVRRFFLDNAQYWISEFHIDALR
                     LDAADQILDHSPMHFLAELSLHIHELRKELNRQVFLFAETAANDVRYIKSRELGGYNL
                     DAQWNDEFHHALRTLLTDERTGYYRDYGEFRHMVKASTEGYVYSGEYSKYRDRRHGSS
                     TRDIPAERFVVFSQNHDQIGNRMLGERLTELVSFEKLKLAAGAVLLSPFIPLLFMGEE
                     YGEEARFQYFISHSDSELIEAVRKGRREEFAAFEWNLEPPDPQDPATFRRAKLNHQLR
                     QEGSHRTLRGFYRELIRLRKTLAPLARLSKAQCEVSAYDKPRVMLMRRWNGDQANLIF
                     YNFSGGEASLAPVIPDGNWRKILDSAASKWDGPGTRLPEILQGGAELSLSISAWSFAL
                     FHAGPIEYNWKEASTL"
     gene            complement(19333..20553)
                     /locus_tag="VF182_03685"
     CDS             complement(19333..20553)
                     /locus_tag="VF182_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6436205.1"
                     /translation="MQSVKHCWRFLAERPVRLTAVLAILLAAVEVFIDWSTWIQLNVS
                     IVYALPLVLAAATRNRRLIWTLALLLISMTFAVYSVQIPAGLFRLNEALFINRVLAAV
                     ALVITAGLLHAWTLAVDAIEVQRRALKYQNERLELAQKQITEKNQELDRRRGEAEEAS
                     RRKTQLLASVSHDIRTPVNSINLIAQLMQRTIEKCGLNSDVLGLTEDLHASAVSLTSL
                     LSEILDLSSIDSGQVHLNESNFSLNELLLEECQQLRPLAQAKGLRLIAEPPPFSIWLR
                     TDQIKLARVLANLVSNAIKFTATGSVTLHVEVTQARGAMIRVRDTGVGIAPANLERIF
                     DEFAQMSDPRRDINKGWGLGLAICRRLIHLMGGNITVESDPNCGSSFTICLPPSSLLD
                     REGENSLKRVSKQA"
     gene            complement(20909..21217)
                     /locus_tag="VF182_03690"
     CDS             complement(20909..21217)
                     /locus_tag="VF182_03690"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6436206.1"
                     /translation="MAALMHYSWPGNIRELENAIEHAFVLCRGQHFGLDDLPQEIGSV
                     GMTDEFIDLNAPGEENLERQRILTTLKFAGGLSEAVRQLGISRTTLRRKLKRIRGLPR
                     "
     gene            complement(21366..21932)
                     /locus_tag="VF182_03695"
                     /pseudo
     CDS             complement(21366..21932)
                     /locus_tag="VF182_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
     gene            22147..23046
                     /locus_tag="VF182_03700"
     CDS             22147..23046
                     /locus_tag="VF182_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6436207.1"
                     /translation="MKTLKRILVGHDLREGGEVAVQSAAALAERCGAALRLVHVVEPL
                     DSYQRISHPLTSPYPVEEIAEKIGARLKALVAGAELAGLETEYEVRTGSSFVELILAQ
                     RAWFADMIVVGGASQPEEPYSPSTSERLMRKADVPVMVAKSRLSTEAKTFLVATDFST
                     GARQAAEQAFMLAENFHGRLVFLHVFDRPSYMVTYVHELGVSLPVPPPTPEQMEPEWE
                     AFLSGLPLEKVEWEKSSIEGHPATAIVRHAEQVKADLVVMGTHGRSGLPYILLGSVAE
                     KVARAASCPVLTIRPEAFQFQMP"
     gene            complement(23146..23400)
                     /locus_tag="VF182_03705"
     CDS             complement(23146..23400)
                     /locus_tag="VF182_03705"
                     /inference="COORDINATES: protein motif:HMM:NF022608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HEX6436208.1"
                     /translation="MTRRCKVGDHVTWNSDAGHVSGKIIKVHENVDDEVYIPTSKEDP
                     RYENRERRPTVSRCSKGGCQKGSAERSSVNCLAEVSAALL"
     gene            complement(23440..25023)
                     /locus_tag="VF182_03710"
     CDS             complement(23440..25023)
                     /locus_tag="VF182_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6436209.1"
                     /translation="MTEVKPELSGPDLVQGIALSTIADGAMLLGHAHGEAALLARRGD
                     ELFAIGNACTHYGAPLNEGLLVEDTVRCPWHHACFSLRTGAAVRAPALNPVSCWRVEQ
                     RDGTVYVRERIKSTGQQATTPAATGVPRSIVVVGGGAAGNAAAEMLRIDGYRGRVTLL
                     SADESLPYDRPNLSKGYLAGSASAESNPLRSTDFYKQNNIDVKLRARVGTIDPASRHV
                     ELVDGSRYAYDALLLATGAEPVRLEIPGGDLPHVHYLRTLADSRVLVDKALAARRAVV
                     IGASFIGLEVAASLRARNLEVTVVGRDTCPMRKVLGADVGNFIRKLHEEHGVTFHFGT
                     TAASIDARQVVLKNGVTLPADLVVIGIGVRPAISLAQEAGLVIDRGIAVDEYLETSIP
                     GIFAAGDIARWPDRLTGERIRIEHWVVAQRQGQTAARNILGRRERFEAVPFFWTEQYD
                     FGLAYVGHAERWDKAEIDGQLDARDCTITYWNGGRKLAVALIHRDLEGLRAEVEFERT
                     IAGLRAADSSSKTIQECHG"
     gene            complement(25059..26183)
                     /gene="tal"
                     /locus_tag="VF182_03715"
     CDS             complement(25059..26183)
                     /gene="tal"
                     /locus_tag="VF182_03715"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX6436210.1"
                     /translation="MKTTQRLHDLHDLGQSLWLDNITRELLTSGTLGRYINDFAVTGV
                     TSNPTIFDKAIRAGRFYDDAIREQTVRGKSGEELFYELALEDLIRAAGLFRPIHDATS
                     GVDGWVSLEVSPLLADDTKGSIKSAVQLHERAQCPNLFIKIPGTREGVPAIEETIFAG
                     VPINVTLLFSREQYTAAAEAYMRGIERRIAAGLDPKVDSVASLFVSRWDVAIKDRVPD
                     RLRNRLGIAIAKRTYKAYRDLLASPRWRKLAGSGARPQRLLWASTGTKDPAASEILYI
                     EALAAPDTINTMPEKTLHAFAEHGQVKGVLPTDGGDAEDVLAEFTREGVHEEMLAADL
                     QSDGIRSFEESWKDLLACVASKSVLVKKRSVQASKEITVT"
     gene            complement(26264..26674)
                     /locus_tag="VF182_03720"
     CDS             complement(26264..26674)
                     /locus_tag="VF182_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436211.1"
                     /translation="MARPMPKPYVVAEEKTDYAHQAYMILKFGFTVAPIIAGLDKFSN
                     LLVDWTQYLTPLVPRWTGIDPAGFMMAIGVIEIIAGILVAVKPKLGALIVTAWLWGII
                     VNLLLVPGYFDVALRDLGLSFGALALARLAKREE"
     gene            27570..28094
                     /locus_tag="VF182_03725"
     CDS             27570..28094
                     /locus_tag="VF182_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436212.1"
                     /translation="MGKAFCRGLVLNGKPVEIAVIQIGSMAAPRLHVTVTGVASGHDL
                     RAGVGETLNRVLGLQADLKGFYRHAAKDARLSILARRFRGVKPPQFPSLLEALVNGIA
                     CQQFSLAAGIQILNRLSEKFGIPVVHAGIRAYTFPRPEDLAVLTPEPLRNLGFSRHKA
                     EAIIELSRAIDSGN"
     gene            28960..29391
                     /locus_tag="VF182_03730"
     CDS             28960..29391
                     /locus_tag="VF182_03730"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX6436213.1"
                     /translation="MPTRKAHARWEGSLKEGNGEVDFGNGLFKGAYSFASRFERGKGT
                     NPEELLGAAHAGCFAMALSLVLGNAGFKPDYVDATAHVTVAPQDGGFKITKSHLVCEA
                     RVPGIEQAVFTEHAQAAKAGCPVSEALAGTEITLDARLVTN"
     gene            29408..29815
                     /locus_tag="VF182_03735"
     CDS             29408..29815
                     /locus_tag="VF182_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX6436214.1"
                     /translation="MIRIKRAYESPARADGRRILVERLWPRGMKKETLEADAWMKEVA
                     PSTELRKWFDHRVERWEKFRQRYRDELDANPDAWQPILDAAERGTVTLLYSAHDTVHN
                     GAVVLRDYLMERVAGRSQRSVRTRARASSRRQA"
     gene            29828..30181
                     /locus_tag="VF182_03740"
     CDS             29828..30181
                     /locus_tag="VF182_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6436215.1"
                     /translation="MSEDEEMYPHATRQIADQRNHLAPGPAAAFQAFSKSVFAEGALP
                     VKTKQLIAVAVAHVTQCPYCIRGHTRAAMRHGATAEEIMEAIWVAAEMRAGGAYAHSA
                     LALDTIAQIQAEKEK"
     gene            30420..32189
                     /locus_tag="VF182_03745"
     CDS             30420..32189
                     /locus_tag="VF182_03745"
                     /EC_number="2.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018495169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC and FAD-binding oxidoreductase
                     domain-containing protein"
                     /protein_id="HEX6436216.1"
                     /translation="MARLVPVNVGLPQDIAWHGETVHTAVWKRAVSGRRMARRLNLDG
                     DGQGDLIGHGGEHRAVFVYQFDCYRYWQRELGRDDFSFGQFGENFTVEGMPDDEVCIG
                     DRYRIGSALFEVTQPRVTCYRVGIRMNEPRMAALLTGSSRPGFYFRVLEEGEVEAGET
                     IVKVTSGAEQMSVAQVNALLYLPGHRREELERALRIPALSGGWKNSFQALLQQELSAR
                     PAGGNPGLSPSDIPLSPPAWRGFRSMRVTAKTRESLDVISLVLEPPDGMPLTAPLPGQ
                     FIVLRLQPQHEAPPLMRSYSLSGAPNAAQYRITIKCGISGAAGTYLDHEIGIGDRLEM
                     SAARGSFTLKPGEHPLVLASAGIGITPVLAILHALAAMRSTREIWWLYAARNRRHHPF
                     ARESRALLRLLPHGRSRIWYSRPTPEDRLGTDFDVRGHLAVTFFDELTIPPEADFYLC
                     GPTEFMRLLSTDLADWGVPPGRIYTEVFGSGESSMPGIVDRHHRLPHSPVGLAGTGPL
                     VSFARSNLSIQWDTKFRSLLELAEACDVPVKWSCRTGVCHRCESGLVAGAVNYRPDPL
                     ESPAEGNVLICCSQPRGDVVIDL"
     gene            32484..33389
                     /locus_tag="VF182_03750"
     CDS             32484..33389
                     /locus_tag="VF182_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6436217.1"
                     /translation="MTMYTRLLIPLDGSDTAETVLPYGRTLARSLEIPVELLGIVDTS
                     VLDNQVFRDTGDSKTIIAQSIRSSEEYLKRIAKTFPADNVRCVVETGKPEDVIIEKAG
                     SDATLTAMATHGRSGINRLLMGSVAEKVLRSAINPLILIRASEEANSEGLAVLKSVIV
                     PLDGSELAETVLSPVVQLANALKLEVILLRAYQVPMNTYAGMEDSYPIDYAKIGGALK
                     DEAQSYLEGKVAELKRNGIEKLSFVISDGSGAGEIIALGRQTPDNLIAMCTHGRSGIK
                     RWVLGSVTEKVVRLCGDPVLIVRAG"
     gene            33516..33887
                     /locus_tag="VF182_03755"
     CDS             33516..33887
                     /locus_tag="VF182_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEX6436218.1"
                     /translation="MIKIKRVYDPPAPRDGFRMLIDRLWPRGLTKEKAYVSEWRKDLA
                     PSDRLRKWFNHDPKKWNEFRRRYHEELRPRAKELQELAQKKRPATITILYGARDQEHN
                     NAVALKELLDELRTGAESGTK"
     gene            34044..35078
                     /locus_tag="VF182_03760"
     CDS             34044..35078
                     /locus_tag="VF182_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX6436219.1"
                     /translation="MSLTQAQAETSFSLKVIPPFRLDLTVWALRRRPENKMDRWDGKT
                     FRRTLMLNGKPAEVAVTQRGSPEEPRLQASVVGVEFGPDTKSGVTETLNWMLGLQVDL
                     KGFYRSAAKDPRLAILAQRFRGVKPPRFPSLLEALVNGIACQQFTLASGIQLLNRLAE
                     DFGRSVIKTGIRVHAFPRAEELAAVKPELLRSLGLNRQKVRAIIELSRAISEGTLDVE
                     GLASLDDDSAVERLSELHGVGRWTAEYVLLRGLGRVEVFPGDDVGARNTLQQWLGLRK
                     PLDYEGVRRLIAKWNPYAGLVYFHMLLFRLNEARYLSCHSNLGRKTQATYPQGWMNPD
                     TLLNVPEAPL"
     gene            complement(35089..35550)
                     /locus_tag="VF182_03765"
     CDS             complement(35089..35550)
                     /locus_tag="VF182_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436220.1"
                     /translation="MQETKVSSGKKSAVLRVRRLAGKRLTFILPAEDETLREFAQESK
                     TGRAAKTLVKEGPLRITLVALKKGTALPSHHVDGPISIQTIRGCLTLTTDRGDMDVPE
                     GNLIALGPGVVHAAKAQEDCAILLTFAMPSPPKIDRRHARDTDEHGKFTGY"
     gene            complement(35685..36308)
                     /locus_tag="VF182_03770"
     CDS             complement(35685..36308)
                     /locus_tag="VF182_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX6436221.1"
                     /translation="MATNEAYQAKQFKLSGLQGISDQTLEMHFKLYEGYVKETNRLTE
                     KISEFLKDGQVDQEEMPAYSELTRRMGFEYNGMVLHEYYFGNMTKGGTGDPGKQSAFY
                     KAAETGFGSYDIWKADFVSIGKMRGVGWAICFQDPKRRRLSNHWVSLHEIGNVAGFEP
                     VLVMDVWEHAFLLDYKPAERPKYIEAFFANIDWKAVEDRLEGNFNKE"
     gene            complement(36398..37066)
                     /locus_tag="VF182_03775"
     CDS             complement(36398..37066)
                     /locus_tag="VF182_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEX6436222.1"
                     /translation="MAPTIRPGQLSEEMIDATLADSFPASDPPSWTLGRDRNEREKTA
                     GAAVTQTSKIHDKEEFIMKANIDLSEEQRGGVVKILNTLLSDEYLLYTKTRNYHWNVT
                     GPQFNDLHKFFEEQYTQLNEVVDQVAERGRTLDGWAFGTMSEFSQHARLKEHPGHYPR
                     AREMIANLVEDHEAVIRQLRSDLETCADKYHDMGTNDFLTGLMEQHEKMAWMLRAFLQ
                     AESV"
     gene            37458..37904
                     /locus_tag="VF182_03780"
     CDS             37458..37904
                     /locus_tag="VF182_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6436223.1"
                     /translation="MRRIVFVLLALVALAGVVAYMTPASGQAAKGTRDELAGKLARRE
                     VQRAQLSIAGREMVQVETLIPAGVESGWHVHPGEEVGYIVEGQVEMRLQGRATVVLGP
                     GDGFLIPPRTPHNARDLGPETGKMLSTYIVETGQPIATFVDPPVQR"
     gene            38190..38639
                     /gene="msrB"
                     /locus_tag="VF182_03785"
     CDS             38190..38639
                     /gene="msrB"
                     /locus_tag="VF182_03785"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX6436224.1"
                     /translation="MTNFGKDPDAISRLSPEQHRVTQQNGTEAPGTGEYLNNKEPGIY
                     VDIVSGEPLFASSDKFESGCGWPSFTKPIEPANVKELRDTTHGMNRTEVRSAHADSHL
                     GHVFPDGPSDRGGLRYCINSASLRFIHRDDMEAQGYGAYLNQVEDVQ"
     gene            38636..39157
                     /gene="msrA"
                     /locus_tag="VF182_03790"
     CDS             38636..39157
                     /gene="msrA"
                     /locus_tag="VF182_03790"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004121324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX6436225.1"
                     /translation="MTEERAVLAGGCFWGVQDLIRSYDGVLSTRVGYTGGTNSHATYR
                     NHPGHAEAVEIIFDPARISYRQILEFFFQIHDPSTRNRQGNDVGTSYRSAIFYTSDEQ
                     KRIAEDTIADVDASGLWPGKVVTEVVPAGDFWEAEPEHQDYLQRYPGGYTCHFVRPNW
                     RVPRRTDEVAKQA"
     gene            39522..40445
                     /locus_tag="VF182_03795"
     CDS             39522..40445
                     /locus_tag="VF182_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX6436226.1"
                     /translation="MAGPILVTGAAGRVGAVGRMVTELLLKQGRAVRAMVRNEDKRAQ
                     ALRDLGAEVVVGDLLDLDSMHRVIAGCETMYFGMSVSDTYLAATVNATAVAKHHGVKA
                     FINMSQMTLSQMSITETTPSPQHKLHWLAEQALNWSGLPVVHVRPTVLLEGFFLIFTA
                     DSVRQSNQIRLPFGEGKTSPIAVEDVAQVLAELLTNPQPHMGKIYHLTGPHSENMHFF
                     AQEYSKALGRTITFEDIPVEVWRVGLLERGLPAHLINHLTTMADLHRAGRYDRMSDDV
                     RTLTGQGPLSVQDFVRKNAAAFTVAPKEANY"
     gene            40463..40945
                     /locus_tag="VF182_03800"
     CDS             40463..40945
                     /locus_tag="VF182_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6130 family protein"
                     /protein_id="HEX6436227.1"
                     /translation="MTNLKHTSLRCAFLLSVTAIVLYAMSAIAQNATDICRPAPVIPL
                     SAGEPAAKIVIDPPLAEPLASRGVAIIHYCAQNLHLVPVFGPNALAVSPRIGHIHVRV
                     DDASWVWADASGNPIILMGLLPGPHKVLIELEDANHRALDKGTVTFVVPEKTAPEKHQ
                     "
     gene            complement(41303..42688)
                     /locus_tag="VF182_03805"
     CDS             complement(41303..42688)
                     /locus_tag="VF182_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEX6436228.1"
                     /translation="MTTTYDAVIIGAGQAGPPLAVRLAGAGRKVAIVERNQFGGTCVN
                     TGCIPTKTLIASARVAHLARRAMEYGVGIGSSVTVDMKRVKERKDAIVRNSNEGLEKW
                     LRGTERLTVYKGHGRFEGARQVRVADQLIEADQIFINVGARANVPALPGLDQVRYLNN
                     SSVMDVDYLPEHLIIVGGSYVGLEFAQMFRRFGSEVTVVEMGPRLIHREDEDVSEAIR
                     EILENESIHIRLDAECIALQNQDDKVVVDLDCSTGEKNVIGSHLLLAVGRTPNTDDLG
                     LEKAGVAIDQGGYVQVDDQLRTNVAGIYALGECNGRGAFTHTSYNDYEIVAANLLNGD
                     RRRVTDRIDAYALYTDPPLGRAGMTEAQVRKSGRKALLATRPMTRVGRAVEKGETQGF
                     MKILVDADTNEILGASLLGIECDEVIHSILDVMYARAPYTVIQRAVHIHPTITELIPT
                     MLGDLKPLDAT"
     gene            complement(43191..43643)
                     /locus_tag="VF182_03810"
     CDS             complement(43191..43643)
                     /locus_tag="VF182_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HEX6436229.1"
                     /translation="MDAEVYPDPRVENFINAHYLPVKIHIKEQPKVFERFGAQWTPTI
                     QVLDPDGSKRHQFEGYLPPDDFLAQLQLGLAHSAFARQQWNEAETAYSDILNTLPQSD
                     AAPEALYWRGVSQYKGSGNPSALQQTAEAFKTRYGDSTWAKKASVWAK"
     gene            complement(43847..44242)
                     /locus_tag="VF182_03815"
     CDS             complement(43847..44242)
                     /locus_tag="VF182_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6436230.1"
                     /translation="MKGYVTNIEDDTLKNKDFRRVLYTGKNSQLVLMSLRPKEEIGEE
                     VHTLDQFIRVEAGSGVAILDGVEHKISDGTAVVIPAGTKHNVINNSDRDELKLYTVYS
                     PPEHRDGTIHKTKADALAREEHFDGKTTE"
     gene            44594..44980
                     /locus_tag="VF182_03820"
     CDS             44594..44980
                     /locus_tag="VF182_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX6436231.1"
                     /translation="MADQKAVLEVVQRFDTAWNRADAEAVAALFAPDGEFVSPSGAIT
                     SARAEIKNLLAGEFQDRLQGTTLTTKVDATKFIKQDAALAKGTFTLRGIDLFLGFETS
                     VSGAFIFRIQKTEGRWMIEKGYILRS"
     gene            complement(45007..45723)
                     /locus_tag="VF182_03825"
     CDS             complement(45007..45723)
                     /locus_tag="VF182_03825"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6436232.1"
                     /translation="MARLPSEATEDHMNARLRGPAFLFDLDGTLVDSVYQHVLAWREA
                     LQASGFELAVWRIHRRIGMSGGLLSNALLRETGHRVSAEEADHLQRLHTEAYARQVSQ
                     IRPLPGARELLAYLSEVGVLFAIATSGRQESARLTLGVLGLSSAVPVITRDQVEHAKP
                     DPDLFLAAAERLGVDVSDAVVVGDSVWDLLAAQRARTLGVGLLSGGYGRDELERAGAY
                     RVYQDPADLLNHLDEVGVRL"
     gene            complement(45780..>46061)
                     /locus_tag="VF182_03830"
     CDS             complement(45780..>46061)
                     /locus_tag="VF182_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012091645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX6436233.1"
                     /translation="FGERPVETDWRLFTTLVRFDAVYVGHFKCNLRRLVDYPKLSRYA
                     RDLFQLPGISGTVNFEHIRKHYYTSHKAINPTGIVPIGPELNFSAGIGK"
BASE COUNT        10257 a        12991 c        12627 g        10186 t
ORIGIN      
        1 ggcgccctcc accaacaacg acgtcaaccg tgtcacatcc acgccgacat gaactcttgc
       61 cctgaactct tcagcttgct ttacggtggc ctttggcggc ctggagttga gcccctagtt
      121 tttgaagttc gttggcgctg acgagctccc ggaccttggg aaagatcttt ccctcttcct
      181 catcttcggc gtggtgttcc acgttatcga tcagctcctc gagtttgtct tcaaaatctt
      241 cacgggccga gagagtagcc atttcctgca ggagcgcttt catagcatgg tgttctttga
      301 gcgacgcggc aaccagttct tttagttcat cgtgcctctg catcgcagga taaaaaatgt
      361 tctcctcgat ctgggcatgg atctccaact cagtcttgat ctgaaggaaa atacgtttct
      421 gctctctgtc atcagcggtt tcagtcctgt cgaataattt ttttactctc gcgtgatcct
      481 gttttaatag ctctaatgca tccatctaac ctccctggac ggctcgaata gagttagcag
      541 ttttcacatt gacgctgccg tttgcgaatc tcgtcgtcga catttgcgcc agaccctgcc
      601 aggtaacgcc tttcgatagg cgcgcggcag gcccgactgt caaaggtttt ccccggctca
      661 cttcacggcg gaattccaac cgcggcttca attcgtaatg ctgctccaac aaacttagct
      721 gagcatcggt gacgtgaggc ttgtcggcga tatcatcctt catgatctct tcagcgggtg
      781 catgggcgct ttgcattgag cggatcccgg gcgaagtcga agcccagcgt ctttccctga
      841 tctggttgat tttcaagtgc cggcggcaaa agcggcgcag cagttccgct gaagatgtcg
      901 gattggtcct cagagctctt ctcctgttcc actcgtttcg aagaaggagg ccgaggagtt
      961 tgcgcttctt cgacggcgtc cggtgacgcc agcgcgcgct gctgcccaat gccagtagcg
     1021 cataaaacag ctcgcctgtt cccgcgaaaa caattagcgc gagtcatcaa tctcgtttga
     1081 ccatactcat tcgaaatacg gggaagatca ccgttgaaac agcaagcgat atgccaaata
     1141 gaaggagagc tcgagtcatt gtctccggta ctcgcattaa tatctcagac cgaatgatgt
     1201 atgacctttc tatatcggcg acgcaaaaat aaataagaca atgttttgac tcctctagta
     1261 aggagccgcc taagagctag tcgaactgct cgcagtgatg tcgcacacga attttctcgc
     1321 gcctcggcag gatagattta gctctctaag agtccgcgat ccgaattgcg cgactgaata
     1381 cgccatcatg taccaggttc ggagttttaa gctctcttat gctcagatct gggacaatca
     1441 acccatgccc gaaccttctc agtaagcatc tccaacgaag tctcggtttc gtacgatctg
     1501 aaacatctcc tatacgtttt tccattatcg gcccacctat ctttttctcg cttctcggag
     1561 tgctagactt catacaaacg taacgagaac gttgtaagcc gatttgacca atggggaaaa
     1621 ccatggaagc tctttacaac ggaaacgtta ttcgggtgag cgcgctacgc gttccgcaca
     1681 agcatcagtg gacatttacg tgggtggtct ggaagcctgg gactgaggcc ggtgtaaaga
     1741 cgtttactga ttacgccggt cgtttcgaca cagcggatga tgccatatcg cgcggctatg
     1801 atttcgcgat gaagtggatc gatgatggca aacccgatct tctcgaagag agataagcgc
     1861 atacttccag ccggctcacc gtttgatctc ggttgaatgg ttaacgggtt ttcgaaaata
     1921 gagctagcgc cagcttcaca tccagatagc gccagggttc accttgtaat ccaactttcc
     1981 ttcctcgaca gagtaaataa gactagtaga actctggaag tcgcctcgct gtaacttcat
     2041 ggcggtcggt gttttcccga atctcttgcc gcaaacagca ccagcatgtc atacatggat
     2101 tgaacgaacg gagagaagca tgacgatttt caacggagag ctcggcctgc aatttgtcac
     2161 ccatttagcc aatcaatata cggtccctca tctggtgcga ctcgctggtc tcgccgcgga
     2221 caagggtttt cagcagatct gggtcaacga taatattcgt taccgcagcc agcttgtcgt
     2281 gctcacagcg attgcatcgc aggtgccggt tcgcctgggc accgccatca tggtgccgta
     2341 ctttcacaat cccttggatg tcgccgactc gctcggcgcg ctttctgagc tgtgcgaagg
     2401 ccaggagata agcgtgggta tcgcccgggg tgatctcggc caatcccccc agcatgttca
     2461 gccgcttcgt cctattgcca tggtcagcga aacagttcag ctgctgcgac aggcattgag
     2521 cggcgaagag ctgagttatg ccgactaccc atttcttcag gagttttacc atctcaatcc
     2581 gaggggaaag tttcgtctcg ctttcaaacc tcgatcgagt tttaagtttt atggcggcgg
     2641 caacggttcg cagtcattgc gaatgtgcgg ccgcatcatg gacggattga tcagctcggg
     2701 aacctatatt cccatgctca aagccggacg gctccccggt atgctcgcga ccgcggatcg
     2761 cgccgcgcag gaagtcgacc gtggaaaacg attgcgcaaa gtatgtgaac ttaacgtctc
     2821 gatttcccgc gaccgtgcta aagcgatcga atttcccaag cgccaggtgg ctcattcaat
     2881 tctgcaatgg gaagctttgg gtttcaccgc cgaagagtac gacaagatgg gcgtaagccg
     2941 ggagcaggtg cttagcctta aaagagcgtt cgaagcggga gccacggtgg aggaagctgc
     3001 taaattggtc accgaacata tggtcaagac ctattacgct gcgggcaggc cggaagaagt
     3061 cagggatcag attatcgaat tggcgggcgc cgcgggaaaa ctcggctacg atcatattgc
     3121 ttttgccaaa ctcggtccgg actatgagga agcgattaat ttactggcgg atcaggtggt
     3181 cccggcatta acgggccggg gactttgagc cgcagcaatg gcaagaacga tttcaatgtt
     3241 tctaacgtgc tcggttcagg ggtggaagtt ttacgggaat cgctgcatca ccgtacatca
     3301 caggccgcta gaagacggta attttattgc attcgcccgg agccttgccc ttaatcgctg
     3361 tgtcactttg aactaagcaa tccggcttca ctcgcatgca atgaggacac gatgagaaaa
     3421 acctctttcg cgacaaccct ctgtttatct cttgcactca ttattggaac aagtctgcag
     3481 gctgaaagta agctcctatc ggcctacatc tccgattcgc caagttcgtc ggttcctgca
     3541 tggatcgcca aggatgcagg cctgttcaag aaatacggtt tggatgtcga cctggtcttc
     3601 atcgacggca gcacccgtgg catacaaagc cttctcgcgg gtgatctgag ctttaccgag
     3661 gcggtgggaa cttcggcgat caacggcaga atcgccgggg gcgatatcgc gattgttaac
     3721 ggtgtggtca atacgttgcc ttattacatc gtcggcaaag gcaacatcaa atcccgtgaa
     3781 gacttgaaag ggagaacggc cgccgtgcac atcccgggaa cggcagccga ttttgctctc
     3841 cgcctggctc tgaaacaggt cggcattcct tataatcaaa tcaaagcgat cactgtgggc
     3901 ggcggtcccg cgcgcattgc ttcggtgatc gcgggtcaga ccgatttcac catcgtcagc
     3961 gatgccgaga agattcaagg agagcgtaac ggcctcaagg tggtgatcga tatggcagct
     4021 ctcaaagtgc cgtttcttta tacttgcacg gtaacgagca ggcaaatgat tcgcgagcaa
     4081 cccaatgcgg tccagcggat ggtcgaggcg attgcagccg gggttcagtt ctttaaagcc
     4141 aacaaagagg aatcgatcaa aatcatgaac aaatatactc gcggcgccaa ccgggagatt
     4201 ctcgaaggag cttattcagc atacagccaa cttttcgtcg aggataccta tcccaccttg
     4261 gaaggattga ggaacactct tgaggtgcaa tcgtcatggg atcccaaagc gggtaaggcc
     4321 aaggcagagg attttgtcga tctgcgcttt gtcgatcgac tgagaagcag cggcttcatc
     4381 gacaaactct acggccgcaa gtaaacggct gataaactgc ggccgccggc cggacttgag
     4441 acgatgcagg acctccaacc taataaggag gacagatcac gatgttgcga acctataata
     4501 acgtaagtgt tccttcgttc atgtatggca cggcgtggaa aaaagaagcc acggcgcgat
     4561 gcgtgttgct ggccgtcgag tcaggcttca acgccatcga caccgccaat caactaatcc
     4621 actaccagga ggctctcgtg ggcgaagcgc tgaagtcgct cgagcagcag ggcgccaagc
     4681 gcgacagcct gtttcttcag accaagttta ctccagccgg gggtcaagac catcggacgc
     4741 cttacgatcc cagcgccgat ctcaccacgc aggtcaacca gtcgcttgcc agctcattga
     4801 atcacctggc tacggactat atcgattcct acgttttaca cgctcccttt tcgcggcgcg
     4861 gtttgggaga agcggactgg gaagtgtggt cggcaatgga agcgctttat gaatcgagga
     4921 aaaccaaaat gatcggcgtt agcaacattg ccgccgggca actcatcgag ctatgccgac
     4981 gggcgaatca taaaccgatg gtggtacaga accgatgttt tgccgcgctc gcatgggaca
     5041 aacagatccg ggaaatctgc caagccgagg acatcatcta tcagggtttt tcgctgttaa
     5101 cggcgaacag ggaagtcctt gtcgattccc aggtacaaaa aatcgccaga agtcaaggcg
     5161 ctacttccgc gcagatcatc ttccgcttcg ccatacaggt cggcatgctg ccgttgaccg
     5221 gaacgacgag ccaacaacac atgagagaag atctggaggc cgaacggctt accctgtcca
     5281 gcgatgagat cgagctgatc gaaaccatcg cggtgtaaat cccctgcgtt tatcttatgt
     5341 cagtacgata gagatcgcat ttagaatcgc accaattctc tgacgttcgc actcctggca
     5401 atgaggactt gcgcgcgaca agttacaaga cacgtggtta tgattctacc tggcactttg
     5461 tcagacatgc ttaggaatta tttcgttaag ctaaggccag cggcggatgt tcctgctgcg
     5521 cgcggcggct ttgaattcct ccgggtccgc ctacgaggat ggtttaagta aattgccgat
     5581 ggctttcgat gagcttctcg aaagcagctt tgtcttcgtc tcgaacagtc gtggcgaatt
     5641 tcgcgtcact ccgtcgccaa cgcgtgagcc acgatggccc taacaatcgc ggcgagagcg
     5701 gatgatcctt cgatccgctc acagtggtaa aagcgatcgt cgtatccgac ttcgggcccg
     5761 tgccgatgcc ttgcgcgaca aaaaagccgg ccgccaggag agctactctc acggcagtcg
     5821 cagccgagta cgtgtagagt tccgccggct cgccccggca atgatcgaaa atccgcgaaa
     5881 acgtctcgac ggtccaaagc gcggcgttgg tcttgtacga gaacggatcg taaaaaataa
     5941 gcttgggagc tttggctgat tccatgacat cggcgaagtt gccctcgagt agttcccact
     6001 ccagcaggtt ggaagtgtgt tcccattttg cgctttcaag aaccctgcgt ggcgcgccgt
     6061 gccgaagatg agggaagcgg ctgggattgg tcagagcaag ccggagcgga tcgaaatccc
     6121 attcgaagct gaccaggcgc aacctgcgaa gcgcctgcgc gccttgtttc gccaagcttg
     6181 tttcaaagca gtgaatcacc gccatggcgt tgaacgcggc gccgaggccg acatcccaga
     6241 tgacgagctc gtttatttcg ctctcctgtt tcattaaccg cgcggcaagg cacgactgct
     6301 ccacatagag tctatgggcc tcggcatccg gagcattcac cgagtgcatg atctcgcccg
     6361 agctgatctg gcggatgctg gagaaacctt cggcaaacgt gcggatttca taatcgccca
     6421 tgcgggtaga ctgcgatgcc ttggcgcttc gcgggcgcct cacgggattg tcttcatcgc
     6481 tgcgcgtgag ctcggatcgt ttggtctcgt aataagccag aaacacatcc gccaaaatac
     6541 tctgtctcat ctcgctcatc aggcgctgat agaaagtcaa attgtgaatc cctagaagat
     6601 gccatcccaa aagttcgtcg cacttgatca gatggtgaat gtagccgcgc gaatgctgtc
     6661 ggcagctgcg gcaatcgcag cgggtatcca gaggttcttg tgaaaactta tgcaccgacc
     6721 tgcgcagctg aagcttgccg tgcgaggtga acgccactcc ccgccgcgcc agttgcgagg
     6781 ggacgataca gtcgaacata tccacgccgc gatgaacgct ctcgagaatg tcgatgggcg
     6841 tgcccacccc cattaaatag cgcgggagat tttccggcag gtgaccggcg accagctccg
     6901 tcacatcgta gcgctcgtga tgagattcgc ctacggccaa acctccaatc gccagcccgt
     6961 caacggcgag cccgcttgag aattcggcgc tgatccttct gagatcggca tggcaggctc
     7021 cctgaacgat gccgaacaga gcttggcgcg aatcgcctct ggcgcgcagc gaacgttggg
     7081 cccacccatg ggtgatctcc attgcccaac gggcctgcgc ataaggcgcc gtcgcaggga
     7141 tgcacacatc cagagccatc atgatgtcgc tgccgatggc tttctgcatc tcgatactcg
     7201 attcgggcga caggagatgc gactggccat cgacataact ttggaaccga gcgccttctt
     7261 cattgatctc tctcgactgc ggcagagaaa aaatctgata tccaccggag tcgctgagca
     7321 ccggcccatg ccagttcatg aaattgtgaa tgccgccgaa gctctttagg acgtccattc
     7381 ctggacgcag caacaggtga tacgtattga ccagaagcac ccgcgcgccc gccgccgtta
     7441 aactctcgac tgtctggcct ttgacggttg cctgagttcc cacgggcatg aagatcggag
     7501 tttgcacatc gccatgaaga gtcgtgaact ttgccgcccg tgccttggat ccactggact
     7561 gcttttccag catgaagttc aattcgcctt tcacgaatcc tgactcccct acatgacaga
     7621 tttaagtgcc gggtctacgg tcttcattgg ccattgcttg tcgatgggtt gatggattgt
     7681 gacgatcact gtgcaggccg ttccagaggc tcgtggtggc aagatcccgc atcttcgaac
     7741 gaaacgttcg gcatgttttc gccgccggcg cacgcctggt cagccggatt aagactgtcg
     7801 ttgaagggcg cgcagagctt ggtgtcgccg cgcattatga tcggccaggc aaattcgcag
     7861 ccgacgccag atgctgaacg gaatcgtaaa gcatcacccg cggacgagcg cccccaattt
     7921 gctgtttcta attcttcgcc gtccatggcg agcccacgtt gaccacttca atttgcgggt
     7981 tctctttgcg aaaatattga agatgccaat cggacgcgaa tagcacgacg gtgtcaccgt
     8041 tttgatcacg gcactgcaat acgccgctgc ccagtgtcat tcgttcgacg ctttggcttt
     8101 tgatccagcg agcgcatgta aatggcaacc gctcgattct ggcctcgacg ccgtactctt
     8161 gccgtaagcg agacaaaacg acctcgaact gtagggctcc cactgccgcg aggatcggct
     8221 cgcggcgggc gccccctgca gcatgcatga cttgcacagc cccctcttcc tccaattgcg
     8281 acaatccctt gtggaactgc ttataacgga cgatctcctg gttgatgaga accgcgaagt
     8341 gttccggcgg aaaacgcgga atgtcgatga gctccgccgg ctctcccgta gttaccgtgt
     8401 cgccaataac gaacagaccc ggattgatga cgccgatgat atcaccggca aacgcttccc
     8461 ggaccggctc ccgttctcgg gcaaagatcc gatatgctcg cgccaggcgc acctcccgtt
     8521 ccaaccgggg atgataggcg gtcatatctt tttcaaaacg accggacaaa atacgcagaa
     8581 aggccatccg atcacgatgc cgcggatcca tgttggcctg gatcttaaaa acaaatccgg
     8641 tgaagcaatc ggcggtgggc tgcagcagag ttccgttctt ctcaaatgcg ctgggctccg
     8701 gcgcgagatt caagagcgcc tccaggaatg gttcgacacc gaaattgttg agcgcgctgc
     8761 caaaaaatac cggcgtgagt tcccctgcca gaaatcgccg ccggtcaaag gcattgcctg
     8821 ctccggtcac aagcgcaatg tgatcacgca gctcactgcc gagcatctcg cccaatagcg
     8881 cagaaagcgc tgggtcgtcg aggtttctga ccgcgaccgg ggcccgccac tgcccgcgct
     8941 cggtcctttc gaactggagc acccggcgat gctgcaggtc atagactcct tggaatgtcg
     9001 atcccatccc gatgggccag ttcatgggcg ctgtcgcgat tcccaatacc cgctcgatct
     9061 cgtccatcag ttccaaagga tcccggccga aatgatccag cttattgatg aaggtcaaaa
     9121 tcggaatgcc gcgcatccgg caaacttgga aaagcttctc tgtttgagct tcgatgcctt
     9181 tggcgctatc gagcaccatg actgcgctat ccgcgaccat gagagtgcga taggtatctt
     9241 cgctgaaatc ctggtgcccg ggtgtatcta aaaggttaag ccggaatcct tgatactcga
     9301 tttccagggc gcttgcagta atcgaaatcc ctcgttcctg ctccaaatcc atccagtctg
     9361 aagtcgcatg gcgctgattt ctacccgcgc gcacggcgcc cgctgtctcc acggcgccgc
     9421 catacaacaa gagtttctct gtgagagtgg tcttgccggc atccggatgc gagatgatcg
     9481 caaaggttcg gcggcgcgcg acctcttctg aaaggcgcgc cgcgccggtt tgttcggttg
     9541 caagttccat agttcctctt cgtgttgatt aacgctggct aggatggttt cgacggacca
     9601 aattcggtcc ggacacgcgg cagaaaagac ctagtgattt cctaagcgtg acggatcaga
     9661 tcagttcatt acattgaaac taagcagtat acagtattct cgcgcgatac gcgattcgag
     9721 gcaggcaatg tgcttccttg cttcgcgtct aaggcagata agtttccgtc gcatcagtct
     9781 tcgggctcgg caaaatccaa tttgctgcca cgcgcccaac ctgcgtcggt gtggcggaaa
     9841 tagtcggcgt gagtcgacac aaacaccttc agatcggctc tccaggatag ccggctcacc
     9901 gagatattgg gatgctgaat acgaatcaca ttgaatgagt ttgcctcgcc ccgcacccgc
     9961 gaagatagag ccgtgcctgc ctggatcatc aatggcgagc gaccactaat cttaaagcgc
    10021 aaagccgtcg gctcgctcag accgatatgc agatggccgg cgagaaacag atcgataccc
    10081 aagccggtaa gctgctgcat cgctctgcgg gcgcgaccga ccaatgcttt atccggatac
    10141 gcctcgggca gatcgaaagg atgatgggtc acgacgattt tggtcacccc attgaaggag
    10201 cccagccgct ctcgcatatg ccgaacctgt cgagagttga cccgcccgcc tttgaaagtt
    10261 gccgaacgcg cggtattgat ccccaatacc gcgatctcag gatcgctata gaacggttga
    10321 agctcatcgc tgatatagcg ccgatatcgg gtgagcttta aaacgaagcg ggcgaacagg
    10381 ttatacagcg ggacgtcgtg gttgccggga acgacgattt tcggaaacgg tatggcatcc
    10441 aagaattccc gggcggcgac aaattgttct ttgcgcgctc gctgcgtcag atcgcctgac
    10501 accgcgacta catccggctg gatccgttcg agcaagctca gcagaggcag gacaatttct
    10561 tgacgcacag tgccgaaatg aagatcggag agatgaacca gagtacgcat agatattagc
    10621 ggccgctttc ttcctggtct gcaggcacga tcacgcgcaa cgcagcgggg tgtatgcaat
    10681 aacggagcgg cagtctcatc accaccactt cgccatccaa cgccactcgc aaacgtctcc
    10741 gccgcgcctc tacggacact tctctgacag acatcgcatc gaagtcacgc gtgtcgccca
    10801 atctgccgaa aagcgcagcc aagccgattt tgagaagccg ccaacgactc atttcgcgcg
    10861 tgacatacaa aaatagtttt ccaccgttga gagatcttcg gctccccata ttcaggccct
    10921 gcaactggta ttcgttatta ccgacaaaga ccatgggcgt gcgacgtctc agttcgcgag
    10981 cttcggtgct gaggcgcact gttaacagcg gatatcgatg aatgataatc agcagggcgg
    11041 acaagaaagc cagccatttg ctccgccctt gcgcttgctg ttgttcgcgc tcatgcacga
    11101 taccggggta aagccccagg ccggaattat tcagaaagta gcggccgttg acctcgccga
    11161 tatcgatctg cgccagccgc tgttgcgcta tgacatccac ggcagattcg agatccagcg
    11221 gcatcccgag atcctttgca agatggttga gcgttccgag aggtaaaatt cccaatatct
    11281 tgttgctacc gacaagttgc gaagcgacag cattaatggt gccgtccccg cctgcggcaa
    11341 cgaccacaga gccttcgacc ttaatggcgc gccgagccag ttcaccgatt tccgcgccgc
    11401 ttcgcgccaa ctggacctcc gctcgcaacc ctttggcagt gaacatttca gccaactgcg
    11461 gatagactgc aacgtcatcg gctttgcgcg cgccagcgtt gacgataaca ataaaatcgg
    11521 tctccatacc tctactgctg cgttctcatg ctcttagtaa atcaacctgg agcatgccct
    11581 gacagcctca gggcggtttc agccgcgacc agctcggagt atctccgacc aagatccaag
    11641 atcgccaaga aagatgattt ccgaacaagc agtcttcgcg cgagcagatt ccgtttcaaa
    11701 ccagctaaat atccgcgttc atcgagtatt ggccgagtcg gcttgtgaag cctcgaaaaa
    11761 actttccatt aaacacgccg caagtcgctc gcgccgggca tggggtcact gcaagcgctg
    11821 ttcgagcggc aaggaagctg cggcgtttgt cgaggccgcg gaactgccct ctagcagcgc
    11881 aaccggaaaa tcggaaaaaa gatccctcaa gtacaagtat ttttttccat taggtgtttt
    11941 atgagcgggc tgcgtttctc cggtgaatac attactccat gccgccgggc tatcggcggg
    12001 cagctcaagc gccgtgtctt cccatgtacc ttgcctcccc aaaggcgcat tctcggcggc
    12061 aaagacaccg gttggaaagc gcggaaccgc aatcacggac caaatggcgc catcatgccg
    12121 ggcgaaggcg cagacccggc tcgcggccgc gccggatacc gcgagcggaa cgtagttgcc
    12181 attttggaac aactctgttt ggcgccgccg aaagttcaac gccttataag tcaaaaacaa
    12241 cttgatccgg ccgtcctccc agtgagtcag aagatccagc agtaaagttt ttagactcgc
    12301 cttctcccgt tctttgatgt cagcgaggaa cttgtctcgc agctcgaagt ccaccggctg
    12361 gcggttatcc gggtcaacga agtgaaggtc ccacaactct gttccttgat agagatcggg
    12421 cgcgcccgga cacgtcaact tgagaagtgt ttgcgacaaa gagttaagcg cgccaaagcg
    12481 cgcgatcttg cgggtccatt ggcgcagctc gccgagaaag gcattgtctt tcgatggctc
    12541 gagaattctt tcaatgaagc cgagaaaagc attttcatag tcggtgtcgg gcttgagcca
    12601 accggtgtgc accttggctt cgcgcaccgc tttgatcgtg taatttttga ttctttcgat
    12661 gaacccggca tgttcctcta cggccagcgg caaggccccg gtcagagact gatagagaaa
    12721 atattcatcg ttgcggtcag gaacttcacg tccttttacc aggcgcttat gcggccggtt
    12781 catctcgccc catgcgaaaa catggcgctc ccagtcctca ggcaattccg acagcacgtt
    12841 gaggcgggcg cgcacgtcct ctcctctctt ggtatcgtgg gttgaagtgg cgttgagact
    12901 atgcggccac gcttgcgccc gccgtaagtt aaattcatga aattcattcg ggctcaagcc
    12961 gaaccggctg ggatcaccgc cgacctcatt gagtgacaag aaacgattgt aaatgtacaa
    13021 ggtggtgtct tcgaagcctt tcgccatcaa cggtccggtt aattgctgca gtcgcatgat
    13081 gaaatcgatc cactggcttt tttcctgctc gcttagctgg tcgccctttt cgagcatgag
    13141 aaatttaccg ataaacgaca gctcaagaat caacccgggg ttcacctcct tggccttgct
    13201 cagggtttcc tgcatgcggt ttcggtcttc agcgccaaag ttttcatggc tgatatagct
    13261 tcgataaacc ggaaaaaatg tcaaaacttc aaccagcgcg cgtttcaagc cataaagcgt
    13321 gatatcgccg ccaaaacggt cccggctcga aactcttttc aacaaatgcg ccagtcgatc
    13381 gacgtcaccg gccatatagc ggccgatgat cagacgtttt ttctcgctga cgagatctgc
    13441 gaacgcaacg ttgtggccgg tgaagcgccg gtacaattcg ctgaacttct gctcgtgatc
    13501 gctatcgcac aatagttgat tgacgaagtt ggcgaattcg tagccggtcg tgccgcatac
    13561 cagccaatca tccggcaggt tctcgcgcat ctccaaaatc ttttcgacga ccacataaac
    13621 ttctccgaca ttttgccgga tgcgcctgag atacgcggct ggatcgtaga gcccatcgat
    13681 atggtcgatc cgcagcccgg tgatccttcc gtcgcgaatc agccgaaaga tcagccgatg
    13741 acagagctcg aatgcattgg catcctctac cctcaatgag atcagctcat tgatattgaa
    13801 gaagcgccgg taattgatct cttcagtggc gactttccag aacgacagcc gataatactg
    13861 ttgcccgagc agttgatcta aagcattgaa cgtctcaggc gcgcccggcc gtccgttgaa
    13921 gcgcgcgata ttttcatcga gaaagttctt gatctcttcg ttggtcgtgt acagctccca
    13981 cagcatgttc ttgacgaaaa tgatttggtc cgcccgctct cttgcttctt cgctggacgg
    14041 cagatttttg agtgtgtaaa gaacacccag aagcttgatc agatcagggt catgacgccc
    14101 caactttcgt cgcaacgcgc cgaggctcgg cgtcaggacc atgctgtagc attctatgtt
    14161 gatcggcagc cgtaactcaa agtaatgcac cgccagtccc tgagcatcat acttcaagac
    14221 aatttcgccg tcttccagac attgaccgta gaatttgccc aggcaaggag ccagaacctt
    14281 gtgatgcatg ttctccatcg ggtaatccca ctcgatgtcg aaataattgg cgtaagccga
    14341 gttcaatcca ttctccaaca cgtccatcag catccagttc tcgctgtgaa aggccatgtg
    14401 gttgggaacg atatcctgga gccagcccat cccctgccgc tgtatctcgc cagctaattg
    14461 atcgaactgc gctgcgccac ccagctcggg gtttatctcg ttcgggtcaa cgacatcgta
    14521 tccatgaagg cttcccgccc gggcgcgaaa aatcggcgaa gcatagacgt cggagatacc
    14581 tagatctcta agatacaaca ctactgcttt tgcgtcggcg aaagtgaagt tccggtggaa
    14641 ctgcaaccgg tatgtggcaa tgggaattcg catgatggag tctttagtta gcgcctgcgc
    14701 gccaacggca tcggtagggc ctcatgactg tcgaataatc taaacaaagc caacgaacgc
    14761 ccctccagat tgtagttggc gcccttgctc acccgccggg tccgccgcgc catcccctcg
    14821 cgagtgtcga gaactacctg ccattgcgct ttcgcgctgg gacccggcag catgaacgcg
    14881 acccgttgat ggtgcgcgtt caacaggaac aagaatgtat cgtcggtgat ccgattgccg
    14941 cggccgtcga gttcgtcgac agcgtcgccc gaaaggatta aaccgacgca gcggttttcc
    15001 gcatcgttcc aatcttcttc cgtcatttct ttgccgtcgc agcggaacca gctcaaatcc
    15061 ttgactgctg tgccacggat actgcgcccc tgaaagaaat ggcgccggcg caaaaccgga
    15121 tgatcgtgaa acaattgaat cagcccgcgc gtaaattcca acaacgattg ccgccgcgga
    15181 tcaagattcc aatcgaccca ggagatctcg ttgtcctggc aataaccatt gttattgccg
    15241 ctttgcgagc ggcctatttc atcgccggcg agaagcatcg gcacgccttg ggataacagc
    15301 agagtggcca aaaagtttct cttctgccgc tctctcaatt cgatgatcgc aatgtcgtcg
    15361 gtggggcctt ccgcgccaca attccagctt gagttgtcgt tgtggccatc gcgattgtct
    15421 tcgccgttgg cttcgttgtg cttgtcgttg taactgacca ggtcgtgcaa agtaaaccca
    15481 tcgtgcgcgg taatgaaatt gatgctcgcg tggggctgac gatcgctggt gccgtacaaa
    15541 tcgctgctgc cggtcaagcg atacgccatg ctgccgactt gtccgctgtc gcctttccag
    15601 aagcggcgca cggtatcgcg gtagataccg ttccattccg cccatagagc cgggaagttg
    15661 cccacctgat atccgccttc gccgagatcc catggttcgg ctatgagttt gacttgagag
    15721 atcaccgggt cctgatgaat aatatcgaag aaggcgccga gccggtccac ggcatgaagc
    15781 tcgcgagcca gagcggaggc caaatcgaaa cgaaacccgt cgacatgcat ctcctgaatc
    15841 caatagcgca aactgtccat gatcaaacgc aggaccgccg gatcagtggc attgagcgta
    15901 ttgccgcagc cggtgtaatc catgtagtaa cgccgatcgt tttccaccag ccgatagtac
    15961 gatgcgttgt cgacgccgcg aaaagacaat gtcggcccca gctcattgcc ttcggcggtg
    16021 tgattgtaaa cgacgtccag aatgacctcg atatcctcgc gatgcagcgt cttgaccagg
    16081 gttttgaatt ccgccacctg ctgccccaac acgccgctgc tcgagtagcg cgcgtcaggg
    16141 gcaaaaaagc cgatggaatt gtaaccccag tagttcgtga ggccccgatc gatcagctgc
    16201 cggtcggcga caaattgatg caccggcatc aactctaccg cagtgacgcc cagagaatga
    16261 aaatattcga gcactgccgg acaggcgagc cccgcatagg tgcctcgtaa ccgctccggc
    16321 actccgggat ggcgcgcggt aaagcccttg acgtgaagct cgtaaatcaa tgtcttgtgc
    16381 catggcgtgc gcggcgccac gtcgttgccc cagctgaacg ccggatctac caccacgcac
    16441 ttcggcagac caacgctgct atcgcgcccg tccggcgcca gatccgcatc gggatgaccg
    16501 atgggataac cgaacagctc atcgccccac ttgatcgttc cggcaatcgc catggcataa
    16561 ggatccaaca gcagcttcgc gggattgaag cgatggcctt gttcgggttc gtaggggcca
    16621 tgcacccgat aaccataaag ctgtccgagg cgaacttcag ggagatagat atgccagaca
    16681 tgcgcggtct tttctcttag cggaatgcgg gcgctctctt taccctctgc cgaatcgaac
    16741 aaacaaagct cgactctttc ggcattctcg gagaacagcg aaaaattgac ccccgcgcca
    16801 tcccatagcg ctccgagtgg ataaggtttg cccggccaga gtttcatagg gtgcttgctt
    16861 ccttccagtt gtactcgatt ggaccggcgt gaaagagcgc gaagctccaa gcgctgatcg
    16921 ataaggacaa ttctgcgccg ccttgcaaaa tttccggcag acgagtgccc ggcccatccc
    16981 acttcgacgc ggcggaatcc aaaatcttcc gccaatttcc gtcaggaata accggcgcga
    17041 gcgaagcctc gccgccactg aaattataaa agatcagatt ggcttgatcg ccattccaac
    17101 gccgcatcaa catgactcgc ggcttgtcgt acgcgctaac ttcgcactga gctttgctca
    17161 agcgcgccag aggcgccagc gttttacgca atctaatcaa ctcacgataa aatccgcgca
    17221 gagttcgatg actgccctcc tggcgcaatt gatgatttaa cttggcacgg cgaaacgtcg
    17281 ctggatcctg gggatccggc ggttcgagat tccattcgaa ggcggcgaat tcctcccgcc
    17341 ggcccttccg taccgcttcg atgagctcgg aatccgaatg gctgataaaa tattgaaatc
    17401 gcgcctcttc accgtactct tcgcccatga ataaaagcgg aatgaaaggc gataacaata
    17461 ccgcgccggc cgccaacttg agcttctcga acgagaccag ctcggtcagt cgctccccca
    17521 gcatgcggtt accgatttga tcgtggtttt gtgaaaagac cacgaagcgc tcggcgggta
    17581 tgtcgcgcgt cgagctgccg tgtctgcggt cgcggtattt cgaatactcg ccggagtata
    17641 cataaccctc cgtggaggct ttgaccatgt ggcgaaactc cccgtagtcg cgatagtaac
    17701 cggtcctctc gtcggtcaag agggtgcgca aagcatgatg aaactcatca ttccattgcg
    17761 cgtcgaggtt gtagccgccg agctcgcgag atttgatgta acggacgtcg ttggcggcgg
    17821 tttcagcaaa tagaaaaacc tgccgattaa gctccttgcg cagttcgtga atatgcaggc
    17881 taagctccgc caggaaatgc atcggcgagt gatcgaggat ctggtcggcc gcgtccaggc
    17941 gcaacgcgtc tatgtgaaat tcactgatcc agtattgcgc gttatcaaga aagaaccggc
    18001 gaacatgatc gcttgcggcg ccgtccagat tgagcgccag gccccatggg gttttgtagc
    18061 gcgaggtgaa atagggaccg taatccgcca ggtaattccc ctccggcccg atatggttgt
    18121 aaacgacatc gaggatcacc gccaaaccgc gctggtgaca ggcatcgaca agacgcttta
    18181 atccctccgg gccgccgtaa gaattttgca ccgcgaatgg ataggcgccg tcgtagcccc
    18241 agtttcgctc gccagggaac tgcgcaagcg gcatcagctc aatcgcggtc actccgagat
    18301 cactgagctg agctaaatga gggatgatcg cctcgaaagt tccttcttct gtataggccc
    18361 cgacatgaag ctcgtagaca agatacttat gcagcggcaa gccaaaccac tgttcatcgt
    18421 tccaggtaaa agatcgatcg actacttgcg acgggccatg gactccttcc ggctgaaatc
    18481 tggaggccgg atccggccgt tcggttgcat gatttagccg gtagaagtac tgcgcgccgg
    18541 ggccaacgtt ttctacctcg gcgcgaaagt aaccatcgcc aacgttcacc agagggacaa
    18601 tccgttcaga cgagccgaga agccgcaagt cgaccgcgga tgcattcctg gcccaaacca
    18661 aaaacaagca gcggttaccg gctaaacagc gcgcgccgat cgcagcgtta gcatcagcct
    18721 tagagtcaaa agagagggga tgaagtgagt catccgagct tttcatgata ataccgataa
    18781 aatctaattt gccgccaggg tcgcgatttc gtcttcaaaa gatctcttga atgtacttgg
    18841 taagtctccc taactcaaag ggcactgaac aaacctgaca atgacagcat attcgccggc
    18901 ggatgagtcc ggctttacgc ggtgtgcccg atacctgcgg aatccacgac tggtcttaac
    18961 atcgtaaaca ccttctatgg tcactacctt aactatggct ttgacgacac acagtttcgc
    19021 gcggtcaccg aggatcgaag cgctcgacgc taaactcgga gaacgacgga taactgttac
    19081 tcacgaagag ttcgcatcgg aggacgatcg ggatgcgtcg gcgagcttga acggtcaggg
    19141 aggaatgttt gtggttatcg aattcttgga aagctacagc tagagcgagt cattgacgtg
    19201 ctggatctct ttcttgtccg gctagcttag agtgaagtat agaaagcgcc taaaataagt
    19261 cgacgaaaca atcggaggtg atttgtgaga tcggagtcac taagaaaagc gtggaaaagg
    19321 catggcgcgt gttcatgcct gtttcgaaac gcgtttcagg gaattctcac cttctcgatc
    19381 gaggagagac gatgggggca gacaaatcgt aaaggaactg ccgcaatttg ggtcgctctc
    19441 gactgtaatg ttgccgccca ttaagtggat cagccggcgg caaatcgcta gtcccaatcc
    19501 ccatcctttg ttgatgtcac gccgcggatc agacatttgg gcgaactcat caaagattcg
    19561 ctccagattc gcgggagcga taccgacacc cgtatcacgg actcggatca tcgcgccgcg
    19621 cgcttgcgtt acctcaacgt gcaatgtcac gctgccggtc gccgtgaatt taatcgcatt
    19681 actcactaga tttgcgagaa cgcgcgcgag cttaatttgg tccgtccgca gccaaataga
    19741 aaacggcggc ggctcagcta tcaaccgcag gcccttggct tgcgccagcg gccgtagctg
    19801 ttggcactcc tccaacaaca gctcgttaag cgagaaattg ctctcgttca aatgaacctg
    19861 acccgaatcg atggaagaga gatcgagaat ttcggagagc aggctcgtaa gcgacacagc
    19921 gctcgcgtga aggtcttctg tcaggcctag aacgtcagaa ttcaacccgc acttttcgat
    19981 cgttcgctgc atcagttgcg cgatcaaatt gatggagtta accggcgtcc ggatatcatg
    20041 cgaaaccgac gcgaggagct gggttttacg gcggctggct tcttcggcct cgccgcggcg
    20101 ccggtcaagt tcctggtttt tttccgtgat ctgcttttgc gccaactcaa gccgttcatt
    20161 ttggtacttc agcgctcgcc gctgcacctc gatggcatcg acggcgagag tccaggcgtg
    20221 aaggagaccg gccgtaataa ccagggctac ggccgcaagc acgcgattta tgaaaagagc
    20281 ctcattcagg cggaagagac ctgcaggaat ctgaacggag tagacggcga aagtcatgga
    20341 gatgaggagc agcgccaatg tccagatgag ccgccggttc cgcgtggccg ccgcgagcac
    20401 cagaggcaga gcgtagacaa ttgaaacgtt gagttgaatc caagtactcc agtcgatgaa
    20461 tacttcgact gccgccagca gaatcgcaag aacagcggtc agccgaacag ggcgctctgc
    20521 gaggaatcgc cagcagtgtt tgacgctttg catttcttct ggagtgtttg cagctcgttt
    20581 gcccttgcaa ttgcgctcat caccgagcca cacgaatgta atgagatgag ttatggagta
    20641 gcgcagcgaa aagtcggtgt caagcaaagt gcatcacgaa gcggcgccga cgagtgacta
    20701 agcacaggcc gcaagaccgc accggcgctc ccgataacgc ttcgctcaca taacggctat
    20761 tgcttcttgt cgaagccgaa cctatagcga agcaacgcca agtcctaccg tcagagtcga
    20821 cagatggtga gctaggcctt ttggctgtag caggcaaaga actgatgtcg ggtgaaacaa
    20881 tcgcagcgtt tcagatgttt tagttgtgtc atctcggtaa gcctcggatc ctcttgagct
    20941 tgcgccgcaa ggtcgtgcgg ctgataccta gctggcgcac cgcttcgctt aatcctccgg
    21001 cgaacttgag tgtggtgagg attctctgcc gctcgagatt ctcttccccc ggagcattta
    21061 gatcgatgaa ctcatctgtc atgccaacgc taccaatctc ttgaggcagg tcatccaagc
    21121 cgaaatgctg acctctgcat aacacgaaag cgtgctcgat ggcgttctcg agctctctaa
    21181 tgttaccggg ccagctataa tgcatcaggg cagccatcgc ttcatgagtg ccaccgagtg
    21241 gagtttttcc ggtttgtcga ttgaacttct cgatgaagtg ctgaaccagc agcgaaatat
    21301 cgtcaacccg ttctctcaag gaaggcaggg tgatgctcac gacttttagc cgatagtaaa
    21361 ggtcatcccg gaagcggccc tctcgcacca gttcccagcg attcttattg ctggcagcga
    21421 tcacgcgcac gtcgactttg ttcgttttac tctcgccgac ccgctcgaac tctttttcct
    21481 gaaggacgcg aagcagtttt acctgtaccg gctggctgac atcgccgatc tcgtccagga
    21541 agattgtgcc gccatcggcc aactcaacgg cccagttttg cgtgtgaggc tccagtgaag
    21601 gcgcctttga tgtgcccgag gagttcactc tcgagaagag tctcgaccaa agacgcgcag
    21661 ttaacggtta caaatggatg atttcgccgc ggactttgct gatggattgc gcgcgctacc
    21721 aattcttttc cggttccact ctcgccgtga acaagcaccg aagcggtgga atccgctacc
    21781 tgctcgatca gcccgtagat ctctctcatt cggtggtttt tcccgacgat ggcgttgaag
    21841 gcggattggc cttttagctg ctcgcgaagc tcatgtattt gctggatatc acgtaacacg
    21901 agaactacgc caccgactcg tccggcgcac atgtgcactc ggacgtcgct ggagctcggc
    21961 ggcggactat cgtgaattag cgcgtgtgtg tgtatgactt cccgccgcgg tcattcacca
    22021 gccgatgggc atgtaggttg cttccgaatg ttctagagaa gagtgtatag acggccacaa
    22081 tacaatctga aacggtcgtt tgtagaaaag ttgtagcgga caattattgc ggaaaagagg
    22141 atctgtatga aaacgctgaa aagaatcctt gttggtcacg acctcaggga aggtggagaa
    22201 gtggctgtac agtcggcagc cgcacttgcg gaacgatgtg gtgctgcgct gcgactggtg
    22261 catgtggtcg agccgctcga ttcctatcaa aggatctctc atccgctgac ttctccttac
    22321 ccggttgagg aaatcgcaga gaaaattgga gcaagactga aagccttggt ggcgggcgcg
    22381 gagcttgccg gcttggaaac ggaatacgag gtgcgcacgg gaagctcttt tgtcgagctt
    22441 atccttgccc agcgcgcctg gttcgctgac atgattgtgg tcggtggcgc atcccaaccg
    22501 gaagagcctt actctcccag taccagcgag cggctgatgc gcaaagccga tgttcccgta
    22561 atggttgcga aaagccgtct cagtactgaa gctaaaacat tcttggtcgc caccgatttc
    22621 tccacaggcg ccagacaggc ggccgagcaa gcgttcatgc tggcagagaa ttttcacggc
    22681 cgtctggttt ttttgcatgt gtttgatcgg ccctcgtata tggttaccta tgtccacgag
    22741 ttaggtgtgt cgctgccggt tccgcctccc acgcctgaac agatggagcc tgaatgggaa
    22801 gctttcctct ccggtttgcc gttagagaaa gtcgagtggg agaaatcttc tatcgaaggc
    22861 catccggcca cagctattgt tcgtcatgca gagcaagtca aagccgatct tgtcgtaatg
    22921 ggtacacacg gcagaagcgg cttaccctat attctcctgg gcagcgtagc cgagaaggta
    22981 gcacgtgcag cgtcttgccc ggtccttacc attaggccgg aggcttttca gttccagatg
    23041 ccatgacggg tgccgagctt aagctgtact ttcctgagct tcaggattcc agactgttct
    23101 taggatcagg atttttcgtg gaatctagct ggcgtgaagt aaagattaaa gtaaggcggc
    23161 ggatacttcc gccagacaat tcactgaaga ccgctcagca gatcctttct gacaaccacc
    23221 ctttgagcat cgcgataccg tgggtcttcg ctctcgattt tcataccgag ggtcttcctt
    23281 gctcgtgggt atatacactt cgtcatcaac attttcgtgc accttaatga tcttgccgct
    23341 gacgtgcccg gcgtccgaat tccaggttac gtgatcgccg accttacatc ttctagtcat
    23401 gttggcttcg actccaaccg cagcgattac gccgatcaat caaccgtggc attcctgaat
    23461 cgttttgctg gaagagtcgg cggctctcaa gccggcgatg gtcctctcga actcgacttc
    23521 cgcccgcagc ccctccaaat cccggtgaat cagcgcgacc gccaatttcc tgcccccgtt
    23581 ccaatacgtg atcgtgcagt ctcgcgcatc aagctgtcca tcgatttcgg ctttatccca
    23641 tcgttcagca tggccgacat aagcgagacc gaaatcgtat tgctcggtcc agaagaacgg
    23701 gacagcctcg aaacgttccc gtcgacccaa tatgttgcgc gcggcagtct gcccctggcg
    23761 ttgcgcaaca acccagtgtt cgatacgaat ccgctcccca gtgagtcggt ccggccaacg
    23821 ggcgatgtcg ccggcggcaa atattccggg aatactcgtt tccaggtact catccaccgc
    23881 gatgccacga tctatgacca gccctgcctc ttgcgccagt gaaatcgccg gtcgcacgcc
    23941 gatgccgatg accacgagat cggccgggag cgttactcca ttcttcaaga cgacctggcg
    24001 cgcgtcaatc gacgcggcgg ttgtgccgaa atgaaacgtc acaccatgtt cttcatggag
    24061 ttttcggatg aaattcccga catcggctcc gaggaccttc ctcatagggc atgtgtctcg
    24121 gccgaccaca gtcacttcga gatttcgcgc tctaagggat gccgcgacct caagtccgat
    24181 gaaactcgcg ccgatcacca ccgcccggcg cgcagccaga gccttatcga caagaacgcg
    24241 gctgtcggcc agcgtacgca agtaatggac gtgcggcagg tcgccgcccg gtatttcgag
    24301 ccgcaccggc tcggcgccag tggcaagcag gagggcatca taagcatacc ggcttccgtc
    24361 cacgagctcg acgtgtcggc tggctggatc gatcgtgccg acgcgcgccc tcagcttcac
    24421 atcgatatta ttctgtttgt agaagtccgt agaccgaagc gggtttgact ccgcggacgc
    24481 actgcccgcg agatagcctt tcgaaagatt gggacggtca taaggcagcg actcatctgc
    24541 gctcagcagg gtgacacggc cgcggtaacc gtctatgcgg agcatctccg cagcggcatt
    24601 gcccgccgcg ccaccaccaa caaccacgat cgaccgcggt acgccggtcg ccgctggtgt
    24661 agtcgcttgc tggcctgtac ttttgattct ttctcgcaca tacaccgtgc cgtctcgctg
    24721 ttcgacgcgc caacaggaga cgggatttaa cgccggcgcg cgcacggccg ccccggtccg
    24781 caagctgaaa caggcgtgat gccagggaca tcgaaccgta tcttcgacaa gcagtccctc
    24841 gttgagcggc gccccgtaat gcgtgcacgc gttaccgatc gcgaacaact cgtcgccccg
    24901 ccgcgcgagc agcgccgctt cgccgtgcgc atggccgagc aacatggcgc cgtcggcaat
    24961 cgtcgaaagt gcgatcccct gcacgagatc aggtccactc aattccggtt ttacttcggt
    25021 cattgaattc tccctacgaa caggcctgag atagcgtctc aggtcactgt tatttcctta
    25081 cttgcttgaa cagacctctt cttcacgaga acgctttttg acgcgacaca cgctaataga
    25141 tccttccagg actcctcgaa agatcggatg ccgtcgctct ggagatcagc ggcaagcatt
    25201 tcctcatgga caccctcccg ggtaaattcg gcgagcacgt cttcggcgtc gccgccatcg
    25261 gtcggcaaca caccttttac ttgaccgtgc tccgcgaagg cgtgcaaggt cttttctggc
    25321 atcgtgttga tcgtatccgg cgccgcgagc gcttcgatgt acaagatctc tgaggcggcc
    25381 gggtccttcg tgccggtgct ggcccagagc aagcgttgcg gccgggctcc ggagccggca
    25441 agttttcgcc aacgcggcga cgccagaagg tcgcgatagg ccttataggt gcgcttggcg
    25501 atcgcaatgc cgagccggtt gcgcagccga tctggaaccc tgtccttgat ggctacgtcc
    25561 cagcggctga cgaagagcga tgccaccgaa tccactttcg gatcaaggcc cgcggcgatg
    25621 cggcgctcga tgccgcgcat ataggcctcg gctgccgcgg tatattgctc ccgcgaaaac
    25681 agcagtgtga cgtttatcgg cacgcccgca aagatcgtct cctcgattgc cggaacgcct
    25741 tcgcgcgtgc ctggaatctt gatgaagaga ttggggcatt gcgcgcgctc atgaagttgt
    25801 acagccgatt tgatgctgcc ctttgtgtca tcggccagca acggagacac ctccaatgaa
    25861 acccagccat caaccccact ggtggcgtcg tggataggac ggaacaggcc ggctgcccgg
    25921 atcaaatcct ctaaggccag ctcatagaac aactcctcgc ccgatttacc ccgaaccgtc
    25981 tgttcacgga tcgcatcgtc gtagaagcgc cccgccctga tcgccttatc gaagatggtt
    26041 ggattggaag ttaccccggt caccgcaaaa tcgttgatgt agcggcccag tgtgccgctg
    26101 gtcagaagct cgcgggtaat attgtcgagc cataggctct ggccgagatc gtgtagatcg
    26161 tgcaatcgtt gcgtcgtctt cattcttcct cccagcgtgc aacgcggcgt ttgctgcgcc
    26221 aagcgtcgag catgagtctt aagattgctt gatgcgggcg gctttattct tcgcgcttgg
    26281 ctaacctggc aagcgcaagc gccccaaaag agagaccgag atcgcgcaac gccacatcaa
    26341 aatacccggg caccagcaac aaatttacaa tgatacccca aagccaggcg gtgacgatca
    26401 aggcgccgag cttcggtttc accgcgacca gaatgccggc aatgatttcg atgactccta
    26461 tggccatcat aaaacccgcc ggatcgatcc cggtccaccg cggcaccagc ggcgtgagat
    26521 attgagtcca gtccaccagc aaattggaaa acttatcgag tccggcgatg atcggtgcga
    26581 cggtgaaccc gaacttcaga atcatataag cctgatgcgc gtagtctgtt ttttcctccg
    26641 cgacgacata aggcttgggc attggacgag ccattttgtt cctcctgcga aagtatttta
    26701 tggatgccgt tcaagcatct atgcagatct caccgcaatg aagatgccaa tgcgaacgag
    26761 aaagttatgt cgtggcagag actagctttt cgcggatcgc tcgcgactga acaatgttgc
    26821 atgaaacatg ctgtaacgtt tcagatgttt cagctgtttc atgccgctga accttgaatc
    26881 tcttgagcct tagcattctg tcgagcattt gcagataaga caggtgcgga gcccgttgcg
    26941 aagcgtagag cacgggttcg tctcaacttt ttctgacgaa ggactgttcc aggtcgatag
    27001 attgggcgag cagcgaacac gcctaagcag gtacatcaca tcgctgatcg agcgcactag
    27061 cccgtactgt cagcgatgtt tttcaccgaa tcgagttcca gcggtgattt acaacaatgc
    27121 cacagtgccc tcacatttgt gcctcatcct tattgcttgt agagaggaga agggtttcga
    27181 tgctttgttt tgaagattgc ctgcccaaag gaatgcattc gttcgagtag cactgccttt
    27241 tagctctccg ggatcatcct tgcgcttacc gatggtcatg gcattgagat tgaaggacta
    27301 aggatgagaa agcatccgtg ctctgagttt gagactgcct gaattagccg gatcagccag
    27361 aaaaaacttt ggccatgctt agagacaaaa tcgggctcgg ataaggtgac gtaccgcaat
    27421 acatcaatcg gctactatgg acaggcaata tgatttgacc cacgcacacg agcggagcag
    27481 tttctcattg aaagtgcttc cacccttccg gcttgatcta acggtctggg cactgcgccg
    27541 ccggccagag aataaaatgg atcgctggga tgggaaaagc attttgcaga gggttggtac
    27601 tcaacggtaa gccggtggaa attgccgtta tccagatagg ctcaatggcc gcgccacgac
    27661 ttcacgtcac cgtaactggc gtagcttccg ggcacgacct gagagcaggt gtcggcgaaa
    27721 cattgaaccg ggtgttgggt ctgcaagcag atctcaaggg cttttatcgg catgctgcaa
    27781 aggatgccag gttgtcgata cttgcccgac gcttccgcgg agttaagccg ccccaattcc
    27841 cttccctgct cgaagccttg gtcaacggca ttgcttgtca acaattcagt ctcgctgccg
    27901 gcattcaaat actcaaccgg ctttccgaga agttcggcat acccgtcgta cacgcaggta
    27961 ttcgcgctta taccttcccc agaccggaag acctcgccgt actcacgccg gagccactgc
    28021 ggaacctcgg cttcagccga cataaagcgg aagcgattat cgagctgagt cgcgctatcg
    28081 attcaggcaa ttagatagag accgcttggt ttctctggat gataagtcgg ccgtcgaaca
    28141 actgtgccgg ctgcgtggcg tggggcgctg gacggcggaa tacgttttgt tgcggggcct
    28201 cggctgattg catctttttc ctggcgacga cgtcggcgcg cggaataact taaaaaaatg
    28261 gcttggcctc aggaaacccc tggattatga cggtgtgcgc cggctgctcg ccaaatgaga
    28321 gtcttacgcc ggacttattt atttccacat gctgctgtac cgccttcatg aaacgggata
    28381 tttgcgcttt cactcaaatg tcgaaggtaa gcgcgacccg aaatcttgac cgtatgagca
    28441 acgtgaagct tcgaaataat ataattggtc gcaccgaagt tagctccaaa agtacctagc
    28501 atgagaaatt cggccgttcg agaactgagc ttcaatccag ttaatattga ccggtgacct
    28561 gaaagtacgc caggagttgt gtttcgcgca tccattatca ttgatcgcat atgcgtgtcg
    28621 cagttgtgga acccgaatgc tcagctgttt tccactctgc cgcgccgggt ttttccgtaa
    28681 aggtgttatc tgccaaggca caaaaattgc gctgtcacgg gtgtgaacac gcggatgccc
    28741 gctgcgaaga aagaaatttc taatcggcgt aggacgacga tagaaaaaca ccggcagcaa
    28801 tacgcggaca gggccgaagt aatataatta taatgctggc ccgctccgat tatcgcgggg
    28861 cgccgcacac tgaaacacgc cggcattgat gacgatgaag atgccggccc ggaagacttc
    28921 accggttaat gaattcaggt tctcttgaaa ggagctgcaa tgccgacacg caaagcgcac
    28981 gcgcggtggg aaggaagcct caaggaaggc aatggcgagg tcgactttgg gaacggtctg
    29041 ttcaagggcg cgtactcgtt cgcatcgcgc ttcgaacgcg gcaagggcac caatcccgaa
    29101 gagctgttgg gcgccgccca tgcgggctgc ttcgcaatgg cgctatcgct ggtattgggc
    29161 aacgcgggct tcaagcccga ttacgtcgat gcgaccgcac atgtcaccgt cgcgccgcag
    29221 gacggcggct ttaagatcac caagagccac ttggtctgcg aggccagggt gcccggcatc
    29281 gaacaggcgg tgtttacgga gcacgcccaa gcagccaagg cgggctgccc ggtatcggaa
    29341 gctctcgccg gcaccgagat cacgctcgac gccaggctcg tgacaaattg accgtgggac
    29401 accgatcatg attcggatca agcgcgcata tgaatccccc gctcgcgcag atggccggcg
    29461 aatcctggtg gaacgtctct ggccgcgcgg catgaagaag gagacccttg aagcggacgc
    29521 gtggatgaag gaagtcgccc ccagcacgga gctacgcaag tggttcgacc atcgggtgga
    29581 gcgctgggag aagtttcggc agcgatatcg ggatgaactg gatgcgaacc ccgacgcctg
    29641 gcaacccatt ctcgacgcgg cggaacgcgg cacggtgacg ctcctgtata gcgcccacga
    29701 cacggttcac aacggcgccg tggtcttacg ggactatctg atggaacgag tagccggacg
    29761 ctctcagcgc agcgttcgaa cacgagcacg agcttcaagc aggcgacaag cgtaaatgga
    29821 ggtatcgatg tcggaagacg aggagatgta tccgcacgcc actcggcaga tcgccgacca
    29881 gcgaaaccat ctggcgcccg ggccggccgc ggccttccag gccttcagca aaagcgtctt
    29941 cgctgaaggt gcgcttcctg tgaagaccaa gcagttgatc gcggttgcgg tcgcgcatgt
    30001 gacgcagtgc ccctattgca tccgcggcca tacccgcgca gcgatgcggc atggggcgac
    30061 ggccgaggag atcatggaag ccatctgggt cgcggcggaa atgcgcgcgg gaggggctta
    30121 cgctcattcg gccctggcgc tggatacgat cgcgcagatc caggcagaaa aagaaaaata
    30181 ggcaaggaaa gatggttgcc agcatcgccg tcaacggcat ggggcgaatc ctgattgtcg
    30241 actgggcgac ggggagttcg ccggcgtcac ctagagccgc cgagcccatt ttcacgcgag
    30301 cttgctgttg acgggcacgc tcttgatcgt tcggacgagg caagtgccta atgagaagca
    30361 cgacgccgat cggctgagcc aagctcatgc gaagctcaaa gggcagggac ggttatggta
    30421 tggcgcggct agtgccggtt aacgtcggcc ttccacaaga tatcgcgtgg catggagaga
    30481 cggtccatac ggcggtgtgg aagcgcgccg tttcgggccg gcgcatggcc cggcggctca
    30541 atctcgacgg cgacggacag ggcgacttga tcggccacgg cggcgaacat cgggcggtct
    30601 ttgtctatca gttcgattgc tatcgctact ggcagcggga acttggccgg gatgacttca
    30661 gcttcggaca gtttggcgag aacttcaccg ttgaaggcat gcccgatgac gaggtgtgca
    30721 tcggcgaccg ctaccggatc gggagcgcgc tattcgaagt gacccagccg cgcgtgacat
    30781 gctaccgcgt cggcatccgg atgaacgagc cccggatggc ggccctgctc accgggagca
    30841 gcaggcccgg cttctacttc cgtgtgcttg aagagggcga ggtcgaggcg ggtgagacga
    30901 tcgttaaagt gacctcgggc gccgagcaga tgagcgtggc gcaagttaac gcgctgctct
    30961 atcttcccgg tcaccgccgc gaagaactcg agcgcgcgct gcgcattccg gcactcagcg
    31021 gcggatggaa gaactcgttt caggctttgc ttcagcaaga gctcagcgcc cgcccggcgg
    31081 gcgggaaccc ggggctttca ccttcagaca tcccgctctc gccccccgcc tggcggggtt
    31141 tccgttccat gcgtgtcacg gcaaaaacgc gtgagagcct cgatgtgatt tcgctcgtgc
    31201 tcgagccgcc ggatgggatg cccctcactg cgccgctgcc ggggcagttc attgtcctgc
    31261 gactgcaacc gcagcacgaa gcgccaccgc tcatgcgcag ctactcgctc tcgggcgcgc
    31321 ccaatgcggc ccaataccgc ataaccatta aatgcgggat tagcggtgcg gccggcactt
    31381 atctcgacca tgagatcggc atcggcgaca ggttggagat gagcgcggca cgaggcagct
    31441 tcacgctcaa accgggcgag cacccgctcg tcctggcgag cgccggcatc ggcatcactc
    31501 cggtgctggc gatactgcac gcgctggccg ccatgcgttc aacacgggaa atctggtggc
    31561 tttacgccgc ccgcaaccgc aggcaccacc cgtttgcccg ggagtcgcgc gccctgctgc
    31621 gcctcctgcc ccatggccgg agccgaatct ggtacagcag accgacgccc gaggatcgac
    31681 ttggcacaga tttcgacgtc cgcggccatc tggcggtaac gttcttcgat gagttgacga
    31741 ttccacccga ggccgatttt tacctgtgcg gccccacgga attcatgcgc ctgctgagca
    31801 cggatctcgc agactggggc gttcctccag ggcgcatcta taccgaagtg ttcggctccg
    31861 gcgaatccag catgcccggc atcgttgatc gccatcatcg acttccgcac tcgcctgtcg
    31921 ggctggcggg gactggcccg ctggtctcgt ttgcgcgcag caatctttcg attcagtggg
    31981 atacgaagtt tcgaagcctg ctggagcttg ccgaagcctg cgatgttccg gtgaaatggt
    32041 cgtgccgcac gggggtgtgc catcgctgcg aaagcggact cgtggccgga gcggtcaact
    32101 accggcccga tccgctcgaa tcacctgccg agggcaacgt gctcatctgc tgctcgcagc
    32161 cgcgcggcga cgtcgtcatc gacctctgag atcaatcatc aacgatgtag aacggcatca
    32221 tcccatgtga cttacgaaac gccacagcgg gtgcgcattt gcgcctcagc tatcgcattt
    32281 agagaaaggc gcttcgctcc tgaccagtgc tgtagcgcaa tggcataatc attgcgttta
    32341 gaggtgtcgg gaaccgagac tgcgagacca agattttgca gggaaaagtc aagccggaat
    32401 tgtaaatcac ccggatcacg cggagacaga aaacagccag gataatctta ccgaaagcat
    32461 cagttcgctt aaggaaaaag agcatgacta tgtacactcg attgttaatt ccgctggacg
    32521 gatcggatac tgctgagacg gtcctgccct acggccgaac tcttgcgcga tcgctcgaaa
    32581 taccggtcga gcttttggga atcgtcgata ccagtgtatt ggacaatcag gtatttcgcg
    32641 acaccggaga ttcgaaaacg atcatagccc aaagcattcg aagcagtgag gagtacctga
    32701 aacgcatagc caaaaccttt cctgccgata acgtaaggtg cgttgtagaa accggcaaac
    32761 cggaagacgt catcattgaa aaagccggat ccgatgcaac gctaacggcc atggccacac
    32821 acggccgttc ggggatcaac cgattgctta tggggagcgt ggcggagaag gtgctgcgaa
    32881 gcgcaatcaa tccgctcatc ctcattcgcg ccagtgaaga agccaatagc gaaggacttg
    32941 ccgtcctaaa atccgtgatc gttcccctcg acggatctga gctggcggaa accgtcctct
    33001 ctcctgtcgt gcagttggcg aacgctttaa aactggaagt cattttgctc cgcgcctacc
    33061 aagtccctat gaacacttac gcgggtatgg aggattccta cccgatcgat tatgcgaaga
    33121 tcggcggcgc gctaaaagac gaagcgcaaa gttacctcga aggcaaagtc gcggaactga
    33181 agcgcaacgg aatcgaaaaa ctctcgttcg tcatatccga cggctccgga gccggtgaga
    33241 tcattgcgct cggccggcaa acccctgaca acctcatagc catgtgcacg cacggacgct
    33301 cggggataaa acgctgggtt ttaggcagcg taaccgaaaa ggtggtacgc ctctgcggcg
    33361 atcctgttct gatcgtccgc gcagggtgag cgtgtcgatt cggttgagcg gctcttaaac
    33421 gccggctgac aacatcatcg cagctatcct tcatcaagat cgtctgttgc cgaatggcag
    33481 cttcgggagc gatgaatcta caacggagac accgcatgat taaaattaaa cgagtttacg
    33541 atccgcctgc accacgggac gggtttcgaa tgcttatcga tcggctttgg ccgcggggac
    33601 tgacgaaaga gaaagcctac gtgagtgagt ggcgcaaaga tttggctccc agcgatcgac
    33661 tgcgaaagtg gttcaaccat gacccgaaga agtggaacga atttagacgg cgctaccacg
    33721 aagagcttcg accaagggca aaagaattgc aggaacttgc acaaaagaaa cgcccggcaa
    33781 cgatcacaat cctttacggc gcgcgagacc aggaacacaa taatgcagtg gctttaaaag
    33841 agctcctcga cgagctgcga acaggtgcgg aatccggcac gaaataaagc gaaggagagg
    33901 cggcttggac gctcggctca aaatccggtg cgaaagaagc ttttcgactt cgagccgaaa
    33961 actttggccc tggttagagg taaaatcggt cgggaaaaaa gtcatgcacc gaaaaatcca
    34021 tcggtactgt ggataaccgc atcatgagtt taacccaagc acaagccgag accagcttct
    34081 cattaaaagt gattccaccg tttcggcttg atctaaccgt ttgggcactg cgccgccggc
    34141 cagagaacaa aatggatcgc tgggacggca agacatttcg cagaacgcta atgctcaacg
    34201 gcaaacccgc ggaagtcgcg gttacgcaga gaggctcgcc tgaggaacca cggctgcaag
    34261 cgtcggtggt aggcgtggag ttcggtcccg atacaaagtc cggagtcacc gagacattga
    34321 actggatgct gggcttgcaa gtggatctga aaggcttcta tcggtccgct gcaaaggatc
    34381 caagactcgc aatacttgcc caacgctttc gtggagttaa gccgccccgc tttccttcat
    34441 tgctcgaggc tttggtgaac ggcattgcct gtcagcagtt cactcttgcc tcgggaattc
    34501 aactgctcaa tcggctggcc gaagacttcg gccgatccgt cataaaaacc ggtattcgcg
    34561 tccatgcctt tccccgtgcg gaagagctcg cagcggtaaa accggagttg ctgcggagcc
    34621 ttgggctcaa tcgacaaaag gtacgggcaa taattgaact gagtcgcgcc atcagcgagg
    34681 gcaccctgga tgtcgagggc ttagcttctc tggatgatga ttcggccgtc gagcgcctga
    34741 gtgagctcca cggcgtgggg cgttggaccg ccgaatacgt tttgctgcgg ggcttaggcc
    34801 gcgtggaggt tttcccaggc gacgatgtgg gcgcccgaaa tactctccaa caatggctcg
    34861 gcctcaggaa gcctctcgat tacgagggcg tgcgccggct gatcgccaaa tggaaccctt
    34921 atgccgggct cgtttatttc catatgctgc tgttccgtct taacgaagcg cgatatttga
    34981 gctgtcactc gaacctcgga cggaaaacac aggccaccta tccccagggt tggatgaacc
    35041 cagacacatt actgaatgta cctgaagctc ctttatagat ctcaaaattt agtagccggt
    35101 gaatttgccg tgttcgtcag tatcccgggc atgcctccga tcgatttttg gcggggacgg
    35161 catcgcaaac gtaagtagta tcgcgcagtc ctcctgcgct tttgcggcgt gcacgacacc
    35221 ggggcccaag gctatcaggt tgccttccgg aacatccatg tcaccccgat cggtcgtgag
    35281 tgttaaacag ccacgaattg tctggatgct gatcggcccg tctacatggt gcgagggaag
    35341 cgcagtgcct ttcttgagag ccacgagtgt aatccgcagg ggtccttcct tgaccagagt
    35401 cttggccgct ctgccggttt tggactcttg ggcaaattct cgcagcgtct cgtcttctgc
    35461 gggcaggatg aaggtaagcc ttttccctgc aaggcggcga actcgaagaa ctgcggattt
    35521 ttttccgctt gacaccttag tctcctgcat aaaacctcct taggactgtc tcaaccaact
    35581 tgtcggcttc tttgcgttgg ctgttaagtg cccgagaata atccgcacat cttcttctgg
    35641 cagagcgcgc ttaattaccc ggcacataac aaccgcaccg agaattattc cttgttgaaa
    35701 tttccttcga gacggtcttc gacggccttc cagtcaatgt tggcgaagaa cgcttcgatg
    35761 tatttgggcc gttccgcggg cttgtaatcg agtaagaacg cgtgttccca tacgtccatt
    35821 accagcaccg gctcgaatcc cgcaacattg ccgatctcat gcaagcttac ccagtgattg
    35881 ctcagccggc ggcgttttgg atcctgaaag cagatcgccc agccgacacc gcgcatctta
    35941 ccgatgctga caaagtcggc tttccaaata tcgtagctgc caaagccggt ctcggccgct
    36001 ttataaaaag ccgactgctt gcccggatca cccgttcccc ctttggtcat gttgccgaag
    36061 tagtactcgt gcagtaccat gccgttgtat tcgaaaccca tacggcgggt gagctctgaa
    36121 taggccggca tctcctcctg atcaacctgg ccatctttta aaaactccga aatcttttct
    36181 gtcagccggt tggtttcttt gacatagccc tcatagagct tgaagtgcat ctcgagggtt
    36241 tgatcggaaa tcccttgaag cccgcttagt ttgaactgct tggcttgata cgcctcattt
    36301 gtcgccatcg ctaacccctc ctttgttttt gaaattctgc gaccgtatag cgtgaagtac
    36361 ccggcatcaa agctggtcca aatgctgaga gcctgggcta gactgactcg gcttgtaaaa
    36421 atgctcttaa catccaggcc attttttcgt gctgttccat caagccggtg agaaaatcgt
    36481 tggtgcccat gtcatgatac ttgtcggcgc aggtctccag atcgctgcgc aattgccgga
    36541 tgactgcctc atggtcctcc acaaggttgg caatcatctc gcgcgccctg ggataatggc
    36601 ccgggtgttc cttgagcctg gcgtgctggg aaaattcgct catggtgccg aatgcccaac
    36661 cgtcaagagt gcgcccgcgt tccgcaacct gatcgacgac ttcattaagc tgggtatatt
    36721 gctcctcgaa aaacttgtgc agatcgttga actgcggtcc ggtgacgttc caatggtagt
    36781 ttcgtgtttt agtgtagaga aggtactcat cagacaacag ggtgttcagg atcttcacaa
    36841 caccgccacg ttgttcctcc gaaaggtcga tatttgcttt cataataaac tcctccttgt
    36901 catgtatttt tgacgtttgc gtgactgcgg cgccggcagt tttttctcgc tcgttacggt
    36961 cgcggcctag tgtccacgac ggcggatcgc tggcgggaaa gctgtccgcc aaagtcgcat
    37021 cgatcatctc ttcagaaagc tgacccggtc taattgtggg tgccatggtt ttctcctttc
    37081 tagtctaacc gacagtttag ctattcatag atgggttgag gtcccctcta aaattcattt
    37141 ttgagccaag tgccagaacg attttagctg ttatgattga cgcagtctta gaacctacta
    37201 cttagcgccg gctactgacg tgtggcaaat actgatgtag gctataacta tgcccgaacc
    37261 aatcaagtag tcggcctcag aaactttgct cgtgtctgaa ggttgtttca gtagatccga
    37321 ggcgaggccc attgctgatc gacagtttgg gactgagttt tcgatgcagg cgtagaggct
    37381 tttgctttca cgttcggctg atccatgatt gccagattag cacggtttta ctgacctata
    37441 ttcgaaggag gcgccgtatg agacggatcg ttttcgtgct gcttgcactt gtagcactag
    37501 ccggagtagt cgcgtacatg acccccgcat ctggacaggc cgctaagggg acacgggacg
    37561 aactggcggg caagcttgcg cggcgcgagg tccaacgcgc gcagttatcg attgcaggtc
    37621 gcgagatggt tcaggtggag acgttgattc cggcgggagt ggagtccgga tggcacgttc
    37681 atccaggaga agaggtcggc tacatcgttg aaggccaggt cgagatgagg ttacagggac
    37741 gtgccaccgt agtattggga ccaggagatg gattcctgat cccgccgcgc actcctcata
    37801 acgcgcgcga cctcggcccg gaaaccggga agatgctgtc gacgtatatc gtcgagacag
    37861 gccagccgat cgcgacgttc gtggacccgc cggttcagag atagatcgcg ctagcgagag
    37921 ccatacgatc ttggaaatcg agcatggccc ctactcactg caactgtcgt gcttgttcct
    37981 acagcgattt atttccgagg gcggcaaact agggacctct taagtaaaag ggtactgctg
    38041 aagcgagtaa gtgcgttgag gatcagtgtg ccactcatcc gtgatggcgt gcttcggcgg
    38101 tgtgcacgat cagtgaaaca ttgctctcgc tggcatcgaa ggcgatgtgt tttttccgac
    38161 ccaatagata agcgaaagga gcattgatca tgacaaattt cggcaaggac cccgacgcga
    38221 tctcaaggct ttcgccggag cagcatcgcg tgactcagca aaacggcacc gaggcccctg
    38281 gaacaggaga atatctgaac aacaaggagc ccggtatcta cgtcgatatt gtgtcgggtg
    38341 agccgctctt cgcatcctca gacaagtttg aatctggatg cggctggcct agcttcacta
    38401 agcccattga acccgcgaac gtaaaggagc tgcgcgacac cacccacggc atgaatcgca
    38461 ccgaggtgcg ctcggcgcac gcggacagcc atctcgggca cgtgtttccc gacggtccga
    38521 gcgaccgcgg cggattgcgc tattgcatca actcagcgtc gctgcggttt atccatcgag
    38581 acgatatgga agcccagggc tatggggctt acttgaacca ggtggaggat gtccaatgac
    38641 tgaggaacgc gctgttcttg ccggcggttg cttctggggc gtgcaggatc ttattcgcag
    38701 ctacgacggg gttctgtcga cacgggtcgg ctacaccggc ggcaccaact cccatgcgac
    38761 gtatcgcaat catcccggac atgcggaagc agtcgagatc atctttgatc ctgcccgtat
    38821 cagttatcgg cagatccttg aattcttctt ccagattcac gacccaagca cgcgcaatcg
    38881 ccagggcaac gacgtcggaa ccagctaccg atctgcgatc ttctacacca gtgacgagca
    38941 gaagcgtatt gccgaagaca ccatcgccga tgttgatgct tcaggcctgt ggccgggcaa
    39001 ggtcgttacg gaagtcgttc cggcaggcga cttctgggag gccgaacccg aacatcagga
    39061 ttatctccag cgatatcccg gcggctacac ctgccacttc gtccggccca actggagagt
    39121 gccgaggcgt accgacgagg ttgcaaagca ggcgtaacag caaatgttca gcaaatcgta
    39181 cgttagagta tgcggcgcaa tgctcggcgc cggtacgtag cacggtacgt gtatttgctc
    39241 ggttgcgggt attagtgcgg caatcacggc tcttgccttc tacttcctga gtgaccttgg
    39301 ccctcacgtg agggcgatcc tcaaggcata cggagccggg gctctcatcg ccatggcagc
    39361 ggaaaccatg atcccggagg cgtttcacaa tgggccccgc tattccggcg ttctcgccgc
    39421 tgccgggttc acagccctaa tccttctcgg caaactcgtt cggttcgcct tgtgagtata
    39481 ccgggacttt cgattcagat taacgcatgg gaggaaacaa tatggcaggt cccattctgg
    39541 tcactggtgc ggcgggccgc gtcggcgccg tcgggcggat ggttacggaa ctgctgttga
    39601 agcaaggccg tgcagtgcgt gcaatggtcc gaaatgaaga caaacgcgcg caggccttgc
    39661 gcgaccttgg tgcggaggtc gtggtcggcg atctgctcga tctcgattcg atgcataggg
    39721 tgattgccgg ctgcgagacg atgtatttcg gcatgtcggt ctcggatacc tacctggccg
    39781 cgactgtcaa tgcgacggcg gtggcgaaac atcacggcgt gaaagcgttc atcaacatgt
    39841 cgcagatgac gctctcacag atgagcatta cggaaaccac tccaagtccg cagcacaagc
    39901 tgcactggct agccgagcaa gcgctgaact ggtccggtct gccggtggtg cacgtccggc
    39961 ccaccgtgct tctcgaaggc ttcttcttga ttttcacagc ggattcggtc aggcagtcaa
    40021 atcagatcag actaccgttc ggcgagggca agacttcgcc gatcgcagtg gaagacgttg
    40081 cacaggtgct cgccgagctg ctcactaatc cgcagccgca catggggaag atctatcacc
    40141 tgacgggtcc gcattcggag aacatgcatt tctttgcgca ggagtattcg aaggcgcttg
    40201 gccgcacgat cacctttgag gatattccgg tcgaggtctg gcgggtcggg ctcctggagc
    40261 gcggcttgcc ggctcacctg ataaatcacc tcacaaccat ggccgatctg caccgtgcgg
    40321 ggcgctacga ccggatgtct gacgacgtgc gcaccctaac gggccaaggg ccgctgagcg
    40381 tgcaggattt cgtcaggaag aatgcagcgg catttaccgt agcgccaaaa gaggcgaact
    40441 actgaacgca tggagaaaga acatgacgaa tctgaaacat acaagcttgc gatgcgcgtt
    40501 tctgttatcg gtcactgcca tcgtgttgta cgcaatgtcc gctatagcgc aaaacgcgac
    40561 ggacatttgc cgacccgccc cggttatccc gctcagcgcc ggtgagcccg cagcgaaaat
    40621 cgttatcgat cctccgctag ccgaaccgct cgcgtcccga ggagtcgcga ttatccacta
    40681 ttgtgctcag aacctgcacc ttgtgccggt gttcggccca aatgctctgg ctgtctcgcc
    40741 gcgcatcgga cacattcatg tgagagtcga tgatgcctcg tgggtgtggg cagatgcgag
    40801 tggtaatcca attatcctga tgggtcttct acccggtccg cacaaagtgc tcatagaatt
    40861 ggaggatgcg aatcaccgcg cccttgacaa aggcacggtc acatttgtcg ttccagaaaa
    40921 gaccgctccc gaaaagcatc agtgaagcgc ctgtctaatg agaggaagaa tggcagacaa
    40981 caccatacgt tcgaaaacgg gaccgggggg cggaatccgc cgcgaatgcc aacctcaaag
    41041 gcgatatcgg tgcccctcga gcattgccag tttgtgcaac tagccggtct gaccgttcac
    41101 tgtcagaact ttgcagaatt attcggccta ggtgatcgat aaagtccacc ggctaagcgc
    41161 cgtcatcgac tatgagtctc catggttgaa cccgaaaaag gcagaccttt atccctaccg
    41221 agtaccaagc ttctagtaca tacatgtcaa cgtcttaaga tccttgttcc atgccaccat
    41281 ataaccaatg tttttgcttc cactaggtag cgtccaacgg cttgagatcg ccgagcatcg
    41341 tcggaatcaa ttccgtgatc gtaggatgga tgtgcaccgc gcgctggatc actgtgtagg
    41401 gcgccctggc atacatcaca tcgagaatcg aatgaatcac ctcatcgcat tcgatgccca
    41461 gtagcgatgc gccgagaatt tcgttagtgt ccgcgtccac cagaattttc atgaatcctt
    41521 gggtctcgcc tttctccacc gctcggccga cccgagtcat aggccgtgtc gcaagtaaag
    41581 ctttgcgccc agactttcgc acttgtgctt cagtcatgcc ggctcgtccc agcggcggat
    41641 ccgtataaag cgcgtaggcg tcgatgcgat cggttacgcg ccgccgatca ccgttcagga
    41701 ggttggcggc aacgatttcg tagtcgttgt aggatgtgtg cgtgaaagct ccccggccgt
    41761 tgcattcgcc cagcgcatag atgccggcca cattggtgcg gagctgatcg tcgacctgaa
    41821 cataaccgcc ctgatcgatc gcgacgccgg ccttttccag gccgagatca tccgtgttgg
    41881 gcgtgcggcc gacggcgaga agcaggtgcg agcctatgac atttttctcg ccagtgctgc
    41941 aatcgagatc gacaacgacc ttatcgtctt gattttgtaa agcgatgcat tcggcatcaa
    42001 gccgaatatg gatgctttcg ttttctagga tctctctaat ggcttcggaa acgtcttcat
    42061 cttcacgatg aatcagccgc ggtcccattt cgacgaccgt gacttcgctg ccgaagcggc
    42121 gaaacatctg agcgaattcc aaacccacat agctgccgcc aacgataatc agatgctcgg
    42181 gaagataatc tacgtccatc acgctcgagt tgttgagata gcgcacctga tcgaggccgg
    42241 ggagcgctgg gacattggcg cgcgcgccga cattgatgaa aatctggtct gcttcgatga
    42301 gttgatcggc gacgcgaacc tggcgcgcgc cttcaaaccg cccatgacct ttatatacgg
    42361 tcagccgttc ggtgcctctg agccacttct ccagaccttc gttggaattg cgtacgatgg
    42421 cgtctttgcg ctcttttacc cgcttcatgt cgaccgtgac cgagctgccg atcccgacgc
    42481 catattccat tgcgcggcgc gccaaatgcg ccacccgggc gctggcgatc aaggttttgg
    42541 tcgggatgca accggtattg acgcacgtgc cgccaaactg gttgcgctcg acgatcgcga
    42601 ctttcctccc ggcgccggcc agccggacgg caagcggcgg ccccgcctgc cccgcgccga
    42661 tgatcactgc gtcataagta gttgtcatta gctatctccg acggctgccc ccatctggcg
    42721 ccaggcgcgc agctattgcg acccagcaaa atctattttt tcagacatag tcgttatcaa
    42781 gctaacatca gcgccgtgtt tcgcagtcag atgttggaat gatgacaact cgtcacccta
    42841 aataaccgtc tccctcgcgc cacggcttcg ctcgagctca tttttagcga ttgttagccc
    42901 taatccgaca atagttcgca ccgtcagcag tgagcgagga cggcgagacc ggaagcagtg
    42961 ggcgccgaac atgcagttga ctctaaggcc tcaacgtacg aaacatcacc cttggggaat
    43021 gcgggccggc cttcaaggca cagtcccatt gcccaccgct gtaggacccg ctggcctcgc
    43081 ggggtaagaa taatatcgga ttacggttag ctgcttaatc acctcataca gtataggaaa
    43141 ttatctattc agtggtcgct gattttgtcc gctaatctgt tgccctctcc ttatttcgcc
    43201 cataccgaag ctttttttgc ccaagtgctg tcaccgtagc gagtcttgaa tgcttctgcg
    43261 gtctgctgca acgcactagg attaccgctg cccttgtact gggaaacacc ccgccagtag
    43321 agcgcttccg gagccgcatc cgattgcgga agtgtgttta aaatgtcgct gtatgcggtc
    43381 tccgcctcgt tccactgctg ccgcgcaaag gccgagtgag ccaggccaag ttgcagttgc
    43441 gccagaaagt cgtccggcgg caaataaccc tcgaactgat ggcgcttgct gccatcgggg
    43501 tctagcactt ggatcgttgg tgtccactgc gcgccgaagc gctcgaatac ttttggttgc
    43561 tccttgatgt ggatctttac ggggaggtaa tgggcattga taaaattctc cacgcgcgga
    43621 tcggggtaaa cctcggcatc cagccgggcg caagctcctc acatcggagt cgcgttaaaa
    43681 tccaccagca gcgggcgctt gctcgatcgc gccttttcca gagcagcatc gatatccttt
    43741 tcccatgcaa ttgacataag cttctccttt tcaatcgggt cccttcatta ctacacggcg
    43801 tccgcgcgcg cttcatccta accggcatag ccgtggcaag actcagctat tcagtcgtct
    43861 tgccgtcaaa atgttcctcg cgcgccagcg catcggcttt ggttttgtga atggttccgt
    43921 cgcggtgctc gggtggagaa taaaccgtat acagcttcaa ttcgtctcga tctgaattat
    43981 tgatgacgtt gtgttttgtg cctgccggga taacgacagc cgtcccgtcc gaaatcttgt
    44041 gctccactcc atcgagaatc gcgacgccgc tgccggcttc gacccggatg aactgatcca
    44101 gcgtatgaac ttcctcgcca atctcctctt ttggccgaag gctcatcaaa accagctgtg
    44161 aatttttccc cgtatataaa actcgcctga aatctttgtt tttcaacgta tcgtcttcaa
    44221 tgttggttac gtaacctttc ataagttgac ctccttcttt gaccctataa ctatcaacca
    44281 accgatcaag catgaaattt atttagctct aagatttctg cgaaccggca tctcactgcc
    44341 tactcatctg ggtgtttctc acgcgctgcg gtgttgtata ttgcgagaca cgcgatgtga
    44401 gatgagccca aaccaatcaa gatcaagagg ctcgcctggg caacgcgatg agtggcgata
    44461 cccttgggaa acaaagtcct agtgacctcg atggtggcat atgtacaaga tcatcgcatc
    44521 aacaacgtta atcacactca cggcctttgc ccccggtgtc caattcgttc gagccggaca
    44581 gccacaggag gtcgtggctg accaaaaagc agtcctggaa gtcgttcaga gattcgatac
    44641 cgcatggaac cgggctgatg cggaagctgt ggctgcgctg tttgcgccgg atggcgagtt
    44701 cgtgagcccc tcgggagcga tcacgagtgc tcgtgccgaa ataaaaaact tgctagccgg
    44761 cgaatttcag gacaggctcc aaggaacaac cctgactacc aaggtcgacg ccaccaaatt
    44821 cataaaacag gacgccgcat tggccaaggg aacatttaca ctcaggggga tcgacctatt
    44881 cctcgggttc gaaacctcgg taagcggcgc gttcattttc aggatccaga aaaccgaagg
    44941 ccgctggatg atcgagaagg gctacatact gcgcagttga cagagccgct tcgagtgacc
    45001 tcggcctcaa agtcggaccc cgacttcatc caggtggttc agcaaatcgg ccgggtcctg
    45061 gtaaacccga taggcgcctg cgcgctcgag ttcatctcgc ccatagccgc cggagagcag
    45121 gcctactcca agggttctgg ctcgttgcgc ggcgagcagg tcccaaacgc tatcgccaac
    45181 gactaccgcg tcgctgacgt cgacgccgag acgctcagcc gccgcgagaa acaaatccgg
    45241 atcgggtttg gcgtgctcga cttgatctcg ggtgatcacc ggcactgcag agctcaaccc
    45301 gagaacgccc aaggtcagac gggcgctctc ttggcgtccg ctggttgcaa tcgcgaacaa
    45361 aacaccgacc tcagacagat acgccagcag ttcccgcgcg ccggggagcg gccgaatctg
    45421 gctcacctga cgggcatagg cttctgtatg gagcctttga agatgatcag cttcttcagc
    45481 gctaactcta tgtccggtct cccgcagtaa tgcgttcgaa agaaggccgc cgctcatgcc
    45541 gatgcgacga tggatacgcc acaccgccag ctcgaatccc gaagcttgca gggcttcgcg
    45601 ccacgcaaga acgtgttgat acacgctatc gacaagagtg ccgtccaggt cgaaaagaaa
    45661 ggccggccca cggagccgcg cgttcatgtg atcttcggtt gcttccgatg gtagccgcgc
    45721 cataagttac tccttcacat cgaatacggc ttggcgtttt cgccttgaag tgtgttcgat
    45781 cacttgccga taccagcaga aaaattcagt tccggcccga tcggaacaat acctgtgggg
    45841 ttaatcgcct tgtgactggt gtaatagtgc ttccgaatgt gttcgaagtt caccgtcccg
    45901 ctgattcccg gtaattggaa cagatcccgg gcgtaacgcg agagcttggg ataatcgacc
    45961 agccggcgca aattgcattt gaagtgaccg acatacacgg catcgaaccg gaccaaggtg
    46021 gtgaacagac gccaatcggt ttcgacgggc cgctcgccaa a
//
LOCUS       DASUVS010000064         3596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1551906, whole genome shotgun sequence.
ACCESSION   DASUVS010000064 DASUVS010000000
VERSION     DASUVS010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551906"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..450)
                     /locus_tag="VF182_03835"
     CDS             complement(304..450)
                     /locus_tag="VF182_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436234.1"
                     /translation="MAIPISRTRASEADRPQQTVGKSLPQFLGLPIIERAIASTAIKA
                     GDES"
     gene            649..1080
                     /locus_tag="VF182_03840"
     CDS             649..1080
                     /locus_tag="VF182_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007942597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX6436235.1"
                     /translation="MILPIWMLVGFAAWTVLLLLATVGVYRWSRILTGRVAIRDFRAD
                     QIEGADWYKRAMRAHANCVENLPVFGAIVFGLYASHVGSATVNALAVAVLLARIMQSL
                     VHVCFIQTNTVTAVRFGFFFAQILSFLWLIGILLSELSGTA"
     gene            complement(1245..1877)
                     /gene="msrA"
                     /locus_tag="VF182_03845"
     CDS             complement(1245..1877)
                     /gene="msrA"
                     /locus_tag="VF182_03845"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX6436236.1"
                     /translation="MRTETLLKPVAKIVAIVLVLGGLAHGAESTSGSAPMAKATFAGG
                     CFWCMEPPYDELDGVISTISGYTGGTKKKPTYEQVSAGTTGHAEAVEITYDPKKISYE
                     KLLDVFWRNIDPLTANAQFCDSGSQYRSAIFYHDSTQKTLAEASKKRVQSRFKQPIVT
                     EIVRAAEFYPAEDYHQDYYKKNPVRYKLYRYGCGRDKRLQELWGDQKNNG"
     gene            2147..2557
                     /locus_tag="VF182_03850"
     CDS             2147..2557
                     /locus_tag="VF182_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX6436237.1"
                     /translation="MKIDGSCHCGFITYEAEIDPQKVGVCHCTDCQSLTGSAFTASVS
                     ADKDAFRLLSGQPKIYVKTAESGAKRAQAFCPQCGSRIYAAAEVDPQSFNLRLGTIRQ
                     RRELRPKTQSWCRSSLDWVMDLGSVPQNAQGRPR"
     gene            complement(2571..>3596)
                     /locus_tag="VF182_03855"
     CDS             complement(2571..>3596)
                     /locus_tag="VF182_03855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX6436238.1"
                     /translation="SQSQMDIQKQFSAGARNGIYWPYLHAPFFTVLLMPLRWFSYVGA
                     FWVWTLFTITLYFVSVVLLIRSGPIRQPPLAASLAVAGAAPVLYWLILTGQTTAIALF
                     IWTMAYRFMQQGRFYWSGFVLGFLSYRAQYLSVILPLLVLRRMWPGIFGFSASFLSLI
                     VLGGLIFSFDSYWQYIEAVGQQAGRIENLTQPLSHYVTAYGFFRQLLPPVWALWGVGL
                     ASSILIYWLWKVWNGVVDFQSGMFDLRYASLLTTTLLLMYHGFVYDLLLLTIPAVLLY
                     QYRSLLSPHYLFVLLFLYLAPYMLLIFRGQFLINPIQPALMFLCFEIYRVSKKLRLRA
                     ADSKYEQ"
BASE COUNT          868 a          943 c          944 g          841 t
ORIGIN      
        1 gatgcgcctg ggcgtgccgt ttttgaagag ctaggcggct actgaaaaaa gcccatatgc
       61 ttcgttgcgc ttgcccgact cgcttcaacg tactgaagcg tacgcctcag ctcatcgaac
      121 ttcgcgcgtc ttgcctctga agtttttgag cagcctgcat attaataagg ccgtccataa
      181 gtaatccggc agtagattta acagttagag cgctttggca atggcattcg gagcagcacg
      241 aacagctgaa tgaattaacg aacctcgaaa gacttaggct cgtgcggagt ttggtgttta
      301 ggtttaggac tcgtcgcctg ctttgatcgc tgttgaggct atcgcccgtt cgataatcgg
      361 caatcccaag aactggggca agctcttacc gaccgtttgc tgcggccggt cagcttcgct
      421 cgctcttgtc cggctgatcg gaattgccat gcactattcg cagtcggcat tttcctgtgc
      481 atcttcgtcg ccacacgaga agattgatcc atcactctca aatccgaagt ttgtgaggag
      541 gtctctggac ggcggattta attttaggat tgaccatgac gctccgcgag cgtcgctcgc
      601 acgcgcattt tatcgcggat ctcatgcgtc acatgatgag gccgcctgat gattttaccc
      661 atatggatgc tggtcggatt cgccgcttgg accgtattgt tgctgctcgc tacagtcggg
      721 gtataccgtt ggagtcggat tctgaccggt cgtgtggcga tacgcgactt ccgcgctgat
      781 cagatcgagg gagcggactg gtacaagcgg gctatgcggg cacatgcaaa ctgcgtcgag
      841 aatctcccgg tttttggcgc gatcgtcttc ggcctgtacg ccagccatgt gggcagcgcc
      901 acggttaatg cgcttgcagt cgcggttctg cttgcccgca tcatgcaatc gctggtgcac
      961 gtatgcttca tccagaccaa cacggtaaca gccgtgcgct tcgggttctt cttcgcgcag
     1021 attctcagtt ttctctggtt gattggaatc ctgctgagcg agctgagtgg aacagcgtaa
     1081 cgtgctccga cgtatcagtg ccgcatgttc aagcgagaac aaagccagtg tcagccttcg
     1141 tgttcaccgc aaccaggagc cgtcggattt tctattcccg gctgatccct cttccgtcac
     1201 gcgtaaaaac tcgacaccgg tcttcagtgg cattgtaacg cacgctaacc gttattcttt
     1261 tgatctcccc aaagctcttg gagccgtttg tcgcggccgc agccatagcg gtaaagcttg
     1321 tagcggaccg ggttcttttt ataatagtcc tgatgataat cttcggcagg atagaattcc
     1381 gcggcgcgaa ctatttcagt cacgatcggc tgtttgaaac gactctggac acgtttcttc
     1441 gaggcttccg caagcgtctt ctgggttgag tcgtgataaa aaatggccga gcgatattgg
     1501 ctgcccgagt cacagaactg cgcattggcc gtcaacggat cgatgttgcg ccaaaacacg
     1561 tcgagcagtt tctcatagct gattttcttt ggatcgtaag tgatctctac cgcttccgcg
     1621 tggccggtag tgccggcgct gacttgctcg taagttggtt tctttttagt gccgccggta
     1681 tatccggaga tcgtagaaat cacgccgtcg agctcatcgt aaggcggttc catacaccag
     1741 aaacagccgc cggcaaacgt tgctttggcc atcggcgcgc tgccggaagt tgactcggcg
     1801 ccatgcgcaa ggcctcccaa aactaaaaca atcgccacaa tttttgccac cggctttaga
     1861 agagtttcag tgcgcatgtc ttcctccgag cagtctccgg cttaacgggt ccatactagt
     1921 ctgcggtaac ccgctctgca atcagacatg aagctagatg gatctccaat gataactgtt
     1981 tgatccttac cccactcgcc atgtgacgaa gttagaatga tccgcgctcg cattcgagac
     2041 tcggcaggcc caactaaaga ttgacacatt cttttctcga cagtagaacc gttctgttct
     2101 gatcgtttga gaaaatgatt aggaatgaca accgtaagga ggagttatga agattgatgg
     2161 tagttgtcac tgtggcttta tcacctatga ggccgaaatc gatccgcaaa aggtcggcgt
     2221 atgtcattgc acggattgcc aaagcctcac gggttcagct tttacggcat ctgtttcggc
     2281 ggacaaggac gctttcaggt tgctcagcgg ccaaccaaaa atttatgtga agaccgcgga
     2341 gagcggcgct aagcgagctc aggctttctg cccgcaatgc ggatcacgga tttatgcggc
     2401 ggccgaggtc gatccgcagt cattcaacct ccggctcgga acgatccgcc aacgacggga
     2461 attgcggccg aaaacacaat cctggtgccg ctcatcgctg gattgggtaa tggatttggg
     2521 ctccgtgccc cagaacgcgc agggcagacc caggtgaacg tacgattgat ctactgctcg
     2581 tatttcgaat ctgccgccct cagccgcaac tttttactga cccggtagat ttcaaagcac
     2641 aagaacataa gcgccggttg gattgggttg atcaagaatt gcccgcgaaa aatcagcagc
     2701 atgtaaggag cgagataaag aaacagcagc acgaataggt aatgaggaga caacaacgat
     2761 ctatactgat acagaagcac ggcggggatg gttaagagca gcagatcata aacgaagccg
     2821 tggtacatga gcagcagtgt tgtcgtgagc aagcttgcat accgaagatc gaacatgccg
     2881 gattgaaaat ccaccacgcc attccatact ttccacaacc agtatatcaa gatagacgat
     2941 gccagcccga cgccccaaag cgcccatacc ggcggcagca gttgccggaa aaatccatac
     3001 gcagtgacat aatgcgataa cggttgtgtg agattttcga tacgacccgc ctgttgaccg
     3061 accgcttcga tatattgcca ataggaatcg aatgagaata tcaaaccacc cagcacgatc
     3121 agcgataaaa atgatgcgct aaatccaaag attcccggcc acattctgcg cagaaccagc
     3181 aatggcagga tgacactcaa atactgggct cggtagctga gaaaacccaa gacaaatcct
     3241 gaccagtaaa atcggccttg ctgcatgaat cgatacgcca tcgtccaaat aaatagagcg
     3301 atcgcggtgg tttgaccagt taagatcagc cagtacagta ccggcgccgc tccagccaca
     3361 gctaagctcg ccgcaagcgg cggttgcctt attggcccgg agcggatcag gagtacgacc
     3421 gatacgaaat aaagggttat cgtaaacaga gtccaaaccc agaacgcgcc gacataagaa
     3481 aaccatctga gcggcatcaa aagcacggta aaaaatggcg cgtgaagata gggccagtag
     3541 attccattac gcgcacccgc gctgaactgc ttttggatgt ccatttgaga ttggct
//
LOCUS       DASUVS010000065         5959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1770080, whole genome shotgun sequence.
ACCESSION   DASUVS010000065 DASUVS010000000
VERSION     DASUVS010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770080"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VF182_03860"
     CDS             <1..309
                     /locus_tag="VF182_03860"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX6436239.1"
                     /translation="CHINELLRSDFDLAKTRYQARFNSEWQQIEMYAIAAQDHRVSFP
                     TVETSFDWRKNEKILVEISRKFDPQRLQEQLRFFDLVPIEHFTDPNRWFSVLLFRKQG
                     "
     gene            complement(384..1028)
                     /locus_tag="VF182_03865"
     CDS             complement(384..1028)
                     /locus_tag="VF182_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX6436240.1"
                     /translation="MAIHLLIKGAMMGFVVAVPVGPLGLLCINRALSMGPWYGLYSGL
                     GIATADALAGGIAALSMTLVSQFLVDHQFAFRLVGGVFLCYLGYEIYYTLPGQPPAQT
                     GRINGLLGTYVATFLLTLSSPVTILSFAAIYAAWHAQSMIGHYGGAAVFAVGVFIGSA
                     SWWVALFLGLTLFRERFNLRLLWWIHRISGGGIAVFGVVLLLSISPLSSILGMP"
     gene            complement(1054..1866)
                     /locus_tag="VF182_03870"
     CDS             complement(1054..1866)
                     /locus_tag="VF182_03870"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX6436241.1"
                     /translation="MSRFFLQQRGLLGLLFLITWIAQTSRTGANDAAEMMRIPAGSFL
                     MGRDDGPADERPQHRITLPEFFIDRLPVTNSQFARFLEAKGSQGSAARQWYDSDDNDA
                     RIHIRAGKWQADLGFENHPVIETSWVGAVAYCDWSGKRLPTEAEWEKSARGTDGRKYS
                     WGNDPPDRARAHFSAGWNDFRPVGSFPKGASPYGVLDLAGNGWEWVSSGYLPYPYDAA
                     DGREDLQRELVRVTRGGGQDSAAEELTTTHRGGHVSRNFRSGHHNIGFRCAR"
     gene            1945..3111
                     /locus_tag="VF182_03875"
     CDS             1945..3111
                     /locus_tag="VF182_03875"
                     /inference="COORDINATES: protein motif:HMM:NF019193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HEX6436242.1"
                     /translation="MRQSPAMRHSQSFRRRGVVEGFFGPVWSMAHRRRLFDFGAARGM
                     NTYLYAPKDDPYHRERWMEPYPQQQWRALLTIIRAAQKRQIDFVYGFHPGKALLFSAE
                     QPLRVLLSKAQRFYDAGVRTFAVLFDDIPSRLEHSADRRRFNHSLARAEGNWLAEIIA
                     RQPPAWADVEWWICPSFYTPDPLLARVFGAFEPHFLENLANYLPPHIPCCWTGPSVVA
                     KKITLAHVRKIAQLVGRPLILWDNYPVNDLSMTDELHIGPLQKRDPHLAQAVYAYLNN
                     PLVQEELSLIPLATCFDYAATPMTYNPEESWNEIVTRTFGAGGSAHWRAIRTFCERAQ
                     RAKRSKHPVRIRSREAKALQDALNYLNAHRQEKWGAEIKPWAEQMERALSTQQQ"
     gene            3175..4605
                     /gene="senA"
                     /locus_tag="VF182_03880"
     CDS             3175..4605
                     /gene="senA"
                     /locus_tag="VF182_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1903257 - selenoneine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine synthase SenA"
                     /protein_id="HEX6436243.1"
                     /translation="MLTTQVGASSRSPFAAMNGAEMIALLREYRSRTRELVADLDERQ
                     LIGPRLATVNPPLWEIGHVGWFQEFWLLRQLRRQTPLLENGDQLYNSTDVAHDVRWEV
                     ALPSREDTWRYLDAVFNRVIEPIEGKSNLSSEELYFYSLTAFHEAMHAEALACTRQSF
                     GYPRPSFMPARTIYENRSEDLFQGDAQIPGGEILVGATAEMPFVFDNEKWAHEVLLRP
                     FRIARAPVSNREFREFVDEGGYFQPKWWSEDGWRWLETGGAPQLENSFAKFFNKPISQ
                     SSQVPAAKAKLEHPLYWRRDPDGHWQERIFDRYVPLQDNLPVVHASWYEAEAFCAWAG
                     RRLPSEVEWETAASAEPTVDGSRLSDTRRLFPWGDTAPTIHEANLDWQNGVVAISDYQ
                     AGDSAFGCRQMIGNIWEWTADNFMAYPGFTPDPYKEYSKPWFGTHKVLRGGSWATSSR
                     LIRNTWRNFYTPDRCDIWAGFRTCAK"
     gene            4615..5571
                     /gene="senB"
                     /locus_tag="VF182_03885"
     CDS             4615..5571
                     /gene="senB"
                     /locus_tag="VF182_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine biosynthesis selenosugar synthase
                     SenB"
                     /protein_id="HEX6436244.1"
                     /translation="MKLELVTPAPLRFNNGNKITALRWAGIFRALGHRVKVSQRYDGK
                     PCDVLIALHARRSYESIRRFYKLYPERPLIVVLTGTDLYRDLRSYRSAQQSLDLATRI
                     VALQKMALAELSESMHPKTRVIYQSAERCFSSYSRDVGDGFKVSVIGHLRKEKDPLRT
                     ALAIRRLPEKSRIMVQHIGRPLNQGLERRARAEMIRNSRYQWLGELPHQKTRQILAQS
                     DITVITSWMEGGSNVLSEALACAVPVIASRISGLMGTLGPDYPGYFAAGDTGALSEML
                     LRAESDRKFYRSLKSICKSLAPLVSPKREVAAWKQLLQELQS"
     gene            complement(5583..>5959)
                     /locus_tag="VF182_03890"
     CDS             complement(5583..>5959)
                     /locus_tag="VF182_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6436245.1"
                     /translation="IFHAVWFLGLLQAASNLGYSAVDWLSLGRYGLYGASMFESFSGG
                     LGTAAFLAFLMNICQKEHATVQYAFLSSIFSLTGRLIGGISGLGAEHYGYGNYFALTF
                     LLSMPAYLLLPWVKPWIREEPS"
BASE COUNT         1402 a         1656 c         1633 g         1268 t
ORIGIN      
        1 tgccatatca acgagctgtt gcggagcgat tttgacctgg cgaaaacgcg ctatcaagca
       61 cgcttcaatt cggaatggca acaaatcgaa atgtacgcca tagccgcgca agatcatcgg
      121 gtaagcttcc ctacagtaga aacatctttc gattggcgga aaaacgaaaa gattcttgtc
      181 gagatcagcc ggaaattcga cccgcagcga ttacaggagc agctaaggtt cttcgatctc
      241 gtaccgattg agcacttcac cgatcccaat cggtggtttt ccgttttgct tttccgaaag
      301 cagggctgat attccacaca ccgaagaacc tggttgtcca cttcggttcg ttagcctgcg
      361 ctcgagctag gctgtccact tggttaaggc atccccaata tcgaactcag gggtgaaata
      421 ctcagcaaca aaacaacgcc gaaaaccgca atgccgccgc ccgagatgcg atggatccac
      481 cataaaagac gcaaattgaa tctctcgcgg aatagcgtta ggccgagaaa caacgccacc
      541 caccacgatg ccgaaccgat aaaaacgccg actgcgaata ccgccgcgcc gccatagtgg
      601 ccaatcatac tctgggcgtg ccacgcggca taaattgcag cgaaagacag gatggtcacg
      661 ggactcgaca aagtcagaag aaacgttgct acgtacgttc cgagaaggcc gttgattctc
      721 ccggtttggg ccggcggctg cccgggaagc gtatagtaaa tctcgtagcc gaggtaacaa
      781 agaaagactc cgccgacgag tcggaaagcg aactgatggt cgactaagaa ctgcgacacc
      841 agagtcatac tcagggcggc aatgcctcca gccaacgcat cggcggtggc gatacccagg
      901 ccggaataaa ggccatacca tggccccatc gacagcgctc gattgatgca caatagacca
      961 agcgggccaa cgggtactgc cacgacaaat cccatcatgg cgcctttgat gagaagatga
     1021 attgccattc cgtatcggcg gttttactct tcgtcagcgc gcgcagcgaa agccaatgtt
     1081 gtgatgccct gaacgaaagt ttcgcgaaac atgaccgcct cgatgggttg tggtcaactc
     1141 ttcggccgcc gaatcctgac cccctcctct ggtgacgcgt acgagttcgc gctgcaagtc
     1201 ttcacggccg tccgccgcat catacggata aggaagatac ccgctgctca cccattccca
     1261 gccgttgccg gcaagatcga gcacgccata agggcttgct cctttcggaa aactccccac
     1321 cggacggaag tcattccatc ctgcgctgaa atgcgcgcgg gcgcgatcgg gcggatcatt
     1381 gccccaggaa tatttgcgcc cgtctgtacc gcgcgccgat ttttcccact ctgcttcggt
     1441 tggcaatcgc ttacccgacc agtcgcagta tgcaactgcg ccgacccacg aagtctcgat
     1501 gacgggatgg ttctcgaagc cgagatcggc ctgccattta ccggcgcgaa tgtgaatacg
     1561 cgcgtcgtta tcatcgctat cgtaccactg gcgggcagcc gaaccttgag atcctttggc
     1621 ctcgaggaat cgggcaaact gcgagtttgt caccggtaac cgatcgatga agaattccgg
     1681 cagagtaatc cggtgttgtg gccgctcgtc agcggggccg tcgtctctgc ccatcagaaa
     1741 tgaacccgca gggattctca tcatttcagc ggcatcgttt gccccggtgc ggctcgtttg
     1801 cgcgatccag gtgatcaaga ataaaagccc gagcaatccc ctttgctgga gaaaaaaacg
     1861 gctcatgctt gcaacataca gcttttgaaa attctaggaa ataccgcgaa acgatcgagc
     1921 taaacttgtc agaggtttaa acctatgcga cagtcccctg caatgcgaca ttcgcaatca
     1981 tttcgccgcc gcggtgttgt cgaaggtttt tttggtccgg tgtggagcat ggcccatcgc
     2041 cgccggctgt tcgattttgg cgcggcccgg ggcatgaaca cttatctcta tgcgcccaag
     2101 gacgacccct atcaccgcga acgctggatg gaaccttatc cgcagcagca atggcgcgcg
     2161 cttttgacga tcattcgcgc agcacaaaag cgccagatcg attttgttta cggctttcat
     2221 ccaggcaagg cacttttgtt tagcgccgag caaccgctgc gcgttctgct atcgaaagcc
     2281 cagcgcttct atgatgcggg cgtgcgtacc ttcgcggtgt tgtttgatga tatcccttcc
     2341 cgattggagc atagcgcgga ccgcaggcgc ttcaaccaca gcctggcgcg agccgaaggg
     2401 aattggctcg cagaaatcat cgctcggcag ccgcctgcat gggcggacgt ggagtggtgg
     2461 atctgtccat cgttttacac gccggatcca ttgctggcgc gcgtgttcgg cgccttcgag
     2521 ccccattttt tggaaaatct cgcgaactac cttccgcccc atattccgtg ctgctggacc
     2581 ggaccttcgg tggtcgcaaa aaaaataacc ctggcgcacg taagaaaaat tgcccagctt
     2641 gtcggccggc cgttgattct ctgggacaac tatccggtga acgatctttc catgaccgac
     2701 gagctccaca taggtccttt gcaaaagcgt gatccgcatc tagcgcaagc tgtttacgcc
     2761 tacctcaaca atccgttggt tcaagaagag ttgagcctta tcccgcttgc cacctgtttt
     2821 gattacgccg caacccccat gacctataat ccggaggaaa gttggaatga gatcgtcacg
     2881 cgaactttcg gtgctggcgg aagtgcgcac tggcgcgcta ttcggacctt ctgcgaaaga
     2941 gctcagcggg ctaaacggtc caagcatccc gtgcggatcc ggagcagaga agcgaaagcg
     3001 ctgcaagatg cactgaatta tttgaatgca catcgtcaag aaaagtgggg cgccgaaatc
     3061 aagccgtggg cagaacagat ggaacgagcg ctcagcacgc aacagcagta ggtggcgcag
     3121 ccatagttcg tcccgaatct tgatcagccg ccgggactgg cgtaatttct aacaatgcta
     3181 accacccaag ttggcgcgtc atcgcgcagc ccatttgccg caatgaatgg cgctgaaatg
     3241 attgcgctgt tgcgagagta tcgttcccgt acccgggagt tggttgcaga ccttgatgag
     3301 cgacaactga tcggacctcg tttagcaacg gtgaacccac cgttgtggga gatcgggcac
     3361 gttggctggt ttcaggagtt ctggctgctg cgtcaactcc ggcggcaaac gcctctgctc
     3421 gagaacggtg atcaactcta caattccacc gatgtggctc atgatgtgcg ttgggaggtc
     3481 gcactgcctt cgcgggagga tacctggcga tatttagatg cagtcttcaa tcgagttatc
     3541 gaacccatcg aaggtaaatc taatttatcc agcgaggagc tttactttta ctctctaacc
     3601 gcatttcacg aagccatgca tgccgaagcg ctggcatgca cacggcaaag cttcggctat
     3661 ccgaggccaa gcttcatgcc cgcccgcacc atctatgaga atcgatctga agatcttttc
     3721 caaggagatg cccaaattcc gggcggcgaa atcttggtcg gcgcgacggc tgagatgccg
     3781 ttcgttttcg acaacgaaaa gtgggcccat gaggtattgc ttcggccgtt ccggatcgcg
     3841 cgagcgcccg tgagcaatag agaatttcga gaattcgtcg atgagggcgg ctatttccag
     3901 ccaaagtggt ggagtgaaga tgggtggcgc tggttggaaa ccggcggcgc gccgcaactc
     3961 gagaactcgt tcgcaaaatt tttcaacaag ccgatcagtc agtcgagcca agtgcctgct
     4021 gcgaaagcga aactcgaaca tccgctgtat tggcgccgcg accctgatgg tcattggcag
     4081 gagcggattt ttgatcgcta cgttccgctg caggacaatc tcccggttgt gcatgccagt
     4141 tggtacgaag ccgaggcgtt ttgcgcttgg gccggacgac gtttaccgag cgaagtagaa
     4201 tgggaaaccg ctgcttcggc agaaccaaca gtggacggtt ccaggttaag cgatactcgg
     4261 cgcctgtttc catggggaga tacagcgccg accattcatg aggcgaatct agattggcag
     4321 aatggcgtcg tcgcgatcag cgactatcaa gcgggcgaca gcgcgtttgg ttgtcgccaa
     4381 atgatcggca acatctggga gtggaccgcc gataacttca tggcgtatcc aggatttacc
     4441 ccggatcctt acaaggaata ctccaagccg tggttcggca cccacaaggt cctgcgcggc
     4501 ggctcctggg cgacatcctc gcggcttatc cgcaatacct ggagaaattt ttatactccg
     4561 gatcgctgcg atatttgggc cggctttcga acctgcgcaa agtgatcgtc cacggtgaaa
     4621 ctcgaactcg tcaccccggc accgttgcgc tttaacaatg gcaacaagat tactgcccta
     4681 cggtgggccg gaatttttag agctcttgga caccgtgtaa aggtatcgca gcgatacgac
     4741 ggcaagcctt gcgatgtcct gatcgcgctc catgccagaa ggagttacga gtcgattcgc
     4801 cgcttttaca aactctatcc cgaacgacct ttgatcgttg ttctaaccgg caccgacctt
     4861 taccgcgatc tccgcagcta ccgcagcgct cagcagtcac tcgacttagc gactcgaatt
     4921 gtcgcgctcc agaagatggc cttggccgag ttgtccgaat ccatgcatcc aaaaacccgg
     4981 gtgatttatc agtccgccga gcgctgtttc agcagctatt ccagggatgt tggagatgga
     5041 ttcaaagttt cagttatcgg acacctgaga aaggagaaag acccgctgcg aaccgcgtta
     5101 gcgatccggc gtttaccgga gaaatcgaga attatggtac agcacatcgg ccggcctctg
     5161 aatcaggggc tcgagcgccg cgcgcgggcc gaaatgatca gaaactcgcg ctaccaatgg
     5221 ctcggggagt tgccgcatca gaagactcgg cagattctag cgcaaagcga tattacggtt
     5281 atcacctcgt ggatggaagg tggctccaat gtattatccg aagcgctggc ctgcgccgtg
     5341 ccggtcatcg cgtcgagaat atcgggactg atggggacct taggacccga ttaccccggc
     5401 tactttgccg ccggggacac gggtgcgttg agcgagatgt tattacgagc cgaaagcgat
     5461 cggaagtttt atcgctcgtt gaaatcgatc tgcaaaagtt tggcgcccct ggtatcgccc
     5521 aagcgtgaag tcgcggcttg gaaacaactt ctacaagagt tgcaatcgta agctgtacag
     5581 ggtcaagaag gctcttcgcg tatccacggt ttcacccagg gcaagagcaa gtaagcgggc
     5641 atcgacaaca agaacgttag agcgaaatag ttgccatagc cgtaatgctc tgcgccgagg
     5701 ccgctgatgc cgccgatcaa gcgtccggta agcgagaaga tcgatgaaag aaaggcatac
     5761 tgcacggttg cgtgctcttt ttgacagatg ttcatcagaa acgccaaaaa tgccgccgtt
     5821 ccgagcccgc cgctgaaaga ctcgaacatg gatgcgccgt aaagaccgta tcttccaagg
     5881 cttaaccagt cgaccgccga ataacccaga ttggaggctg cttggagcaa gcccaaaaac
     5941 catactgcat ggaagattc
//
LOCUS       DASUVS010000066        10826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1334487, whole genome shotgun sequence.
ACCESSION   DASUVS010000066 DASUVS010000000
VERSION     DASUVS010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10826
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334487"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1632
                     /gene="mutS"
                     /locus_tag="VF182_03895"
     CDS             <1..1632
                     /gene="mutS"
                     /locus_tag="VF182_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX6436246.1"
                     /translation="QAGVQALVENYRLREDLKRALQNVQDLERLAARTVAGTASPRDL
                     VAIKQTLLALAELQSLLAGENSDILRALREQLSVLPEIVELISASVVDDPPFSLRDGG
                     FIRQGFDSELDDIRGGRSQAKQWIASFEVAERRRTGINSLKVRYNRVFGYYIEITSSN
                     LKAVPEDYIRKQTLVNGERYITPELKEYETRVLNSQSLLEKLETELLGRVRGQVAAAY
                     PVLKKSSMALASIDVLLSLAEVAESCHWVAPWLDDGLMLTVREGRHPVVEAAIGRGAF
                     VPNDCVIDPNEQQILLLTGPNMAGKSTYMRQIALIAVLAQMGSFVPAREARIGLVDRV
                     FTRIGASDSLARGESTFMIEMKETAAILEHATQRSLILLDEVGRGTSTFDGISIAWAV
                     AESLHERTERPRTLFATHYHELTDLAHTKERIRNYNFAVKEWQGDIIFLRNLVEGAAS
                     RSYGIHVARLAGLPAALIDHAKAILAQLEGGGDECRGRLASMRPSAGEQPLQMALFAS
                     AEDRFREQLRSLDVSTITPVEALNILYKLTEEAKE"
     gene            1655..3001
                     /locus_tag="VF182_03900"
     CDS             1655..3001
                     /locus_tag="VF182_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX6436247.1"
                     /translation="MTIRNLRYIAIATAIVALLGMSLVHFDSFAAAQSNKKARAASPG
                     KSTEVDPPSSRDGGQSSSDELKPASQLSTDPADKSLISSVRFISTARYTRVLLDLSKE
                     AKYEIHRLEGDKSKGLAARTYVDISGARLGTNSKEPLAVGDRLLRQIRVGQYTDNVVR
                     VVLDLADDTGRYDISLLPDLYRLVIHIQPERTGETVKPGADKPVIRATAKTKQPAKTT
                     VSGIRKIVLDPGHGGKDPGAIGVGGVAEKDLVLSIAKKLAVKVKREMGIEVILTRKDD
                     RYVPLEDRTATANAEDADLFVSLHMNASPNLEARGLETYYLDNTSDEASIRLAARENS
                     TSRKNISDLQFILSDMMQNVKLEDSISLAHHLQGAVVSGMSRKMSDVKDLGVKKALFY
                     VLVGARMPSVLVEMFFITNRLEGPAMSDEGHQDAMVDSLLEGIRKYTQGSVATKTL"
     gene            3016..5721
                     /gene="glnD"
                     /locus_tag="VF182_03905"
     CDS             3016..5721
                     /gene="glnD"
                     /locus_tag="VF182_03905"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX6436248.1"
                     /translation="MDFTSLPDSLLETTKDLSDLPGFTRRYVQKARTVLFEKHRAGAG
                     GLEIAAAYSKMMDELICHLFGGISKDFQSRYPTGNPQCAIIAQGGYGRSELNPHSDID
                     LLFIYSWKVSPFVEAVTERLLYTLWDAGLQVGHAIRSISESMRLASTDMKVRTALLDC
                     RYVCGDQTLYNEFEKAVQSRLARKRVSGFVGEKLAENDARHAGYGGSVYLLEPDVKES
                     EGGLRDVHTARWMARVKMQVKDLDELVGKGIISAADVVKLKASHDFLLRVRNELHFST
                     GKHQDQLTFEEQEKVSQALGFQGEGSLKAVEVFMSQYYRHASEISRLTTLVTHRLTDS
                     SWPLIGGMVGLGRTIRDGVRISRDQITITKPEILTQDRGNLMRIFEDTQKHRCSLSHE
                     SRELLREHLHLIDDQFRASAAANLPFFRILKWKEGVYDTLFEMHRAGVLGAFVPEFGR
                     LLCMVLHDAYHIYTVDQHSLRLIQEIERLKAGEFKESLPLLTQLAREAEKIELLYLGL
                     MLHDIGKGFGGGHSERGAVMVRRIAERMRLNVDDGALVEFLVRHHLLMTHTAFRRDLE
                     DEKTICDFAQTMGSVNNLKMLYLLTYADVRGVGPDVWNPWKASLLGELYVKTLTVLED
                     MEKGEFQRQDIRAALGRIQTRVREELLATSSPEEADCFIRVMPERYFLSVTEGEIPAQ
                     FALMQEYKGRGAAVSVEHFPEKDCSTVAICTTDRPGLFASIAGVMAAMRLDILNARIF
                     TASDGRILDVFRVSHGGRSELAMAEQRWSRFSSTLEAVLEGNIDVEQLVEKSQSTLLR
                     KRMPKVLTSIEIDNEASAHYTVVEVYTADRLGVLFRITYTLHQLGLSIHVAKISTNVD
                     QVADVFFVTTERGDKVEDSARIEAIRQTLHASLVPDDERIAAPLH"
     gene            5690..6628
                     /gene="xerD"
                     /locus_tag="VF182_03910"
     CDS             5690..6628
                     /gene="xerD"
                     /locus_tag="VF182_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX6436249.1"
                     /translation="MMKESLHPYIDSFLTMISVEKGLANNTVQAYSRDLSGLAEFVLG
                     QKITRWDAVEAIHLRLFVSTLRARHLSSRSIARQIVTVRRFYRFLETEGLVSNPPVPE
                     FHLSATARKLPHALNGGDIRSLLNQPDPNQILGARDQAMLELLYATGLRVSELVLLKL
                     QQVDFQGDYLTVKGKGSKMRAVPFGKWAREKLLRYIQETRPRLLKGKSSAFLFLNRSG
                     QALSRQGFWKIIRGYAMAAGIDKKVSPHTLRHSFATHLLAGGADLRSVQTMLGHADIS
                     TTQIYTHVDANRLKQAHQRYHPREGGVQRKPKPAPD"
     gene            6732..7403
                     /locus_tag="VF182_03915"
     CDS             6732..7403
                     /locus_tag="VF182_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014029404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX6436250.1"
                     /translation="MEETVRQIAIWALPVLLAIVFHEVAHGWVAYHLGDSTAARMGRL
                     TLNPLAHVDLFGTILIPLLLILSNAPFLFGYAKPVPVNFQALRNPKRDMIWVALAGPV
                     TNFLLAFICVLLLKFLVPMIGKSSPVGMSASLVDFFVPIVFMLERGILINIVLAVFNL
                     LPLPPLDGGRVLVGLLPEPFSTLLARIEPFGFLILLVLLMTRSLSWIIGPPLDFLLKV
                     FLELI"
     gene            7415..8392
                     /gene="trpS"
                     /locus_tag="VF182_03920"
     CDS             7415..8392
                     /gene="trpS"
                     /locus_tag="VF182_03920"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX6436251.1"
                     /translation="METIVSGARPTGRLHLGHLHGALRNWIRLQENYRCYFFVADWHA
                     LTTDYASPQGIKQSTIEMVMDWLSVGLDPARSVLFRQSRVKEHAELHLIYSMITPVPW
                     LERNPTYKEQIKELTGKDLSTYGFLGYPVLQAADITIYKANKVPVGVDQAPHVELTRE
                     IVRRFNQLYRSIFPEPEVLLTETQKLPGLDGRKMSKSYGNAVFLSDPAEEIDRKLSRM
                     MTDPARVKRTDPGEPEKCPAFQLHKIYCTPEEIEFVSTGCRTAGIGCLECKKIMIKHV
                     IEDLAPFREKRAQLEKTPEVVEEVLQAGHQAAQKKASETMSEVRETLGL"
     gene            8389..9159
                     /locus_tag="VF182_03925"
     CDS             8389..9159
                     /locus_tag="VF182_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX6436252.1"
                     /translation="MSTRVQLEIFEGPLDLLLHLIKKNEVSITDIPIAGITEQYLATL
                     ELMETLSLDVAGEFLVMAATLIHIKSRMLLPAGADEEDEDEGDDPRDELVRRLLEYQR
                     FKDAADQLEQREILSRDVFVRSATPVEEAAAPTFRELSVFELLNALRRVIDRLPKDVF
                     HQVELDKVTVREKMTLLLDKLRAQGRVLFEELFNEAKTRMDVVVTFLAMLELVKVRAV
                     RIFQEELTGPIIIEAAASMEQAAELANAVEAEEEQHGT"
     gene            9149..9778
                     /gene="scpB"
                     /locus_tag="VF182_03930"
     CDS             9149..9778
                     /gene="scpB"
                     /locus_tag="VF182_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX6436253.1"
                     /translation="MEREDVKSILESLLFVADGPLTVQRLTEVIEGAGKDDIRSILDE
                     MDNELQDNRRGIRLVEVAGGYQLRTAKANADWVKKFLGGRPARMGRATLETLAIVAYR
                     QPITKAEIEAIRGVDVDGVITTLLERNLVRAVARKDVPGRPFLYGTTPEFLELFNLKD
                     LAQLPTLKEMEEMTLPEIPGDQVSMEQIEDADQPQEAEQPQEAEQEKQD"
     gene            9785..10570
                     /locus_tag="VF182_03935"
     CDS             9785..10570
                     /locus_tag="VF182_03935"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX6436254.1"
                     /translation="MERLQKILARAGLASRREAERWILQGRVTVNGTVVRKMGTQAEP
                     SRDSIRVDGKRIRLATEPVYLALNKPAGFITTLNDPQQRPDITQFLEPLGEKQRVFPV
                     GRLDFNSSGLILLTNDGELAQRLMHPRFGVKKLYHVKLSACPTEIELGRLRKGIRLED
                     GMTAPARVRVLEKLKKNAWVEIEIHEGRNREVRRMFETLGYFVEKLIRVRIGNVELGS
                     LAPGEIRPLMRTEIIKLKRAVGLQDRRSEPERPERRRRAPSRR"
BASE COUNT         2442 a         2871 c         3124 g         2389 t
ORIGIN      
        1 caagccggcg tccaggcgct ggttgaaaat tatcgattgc gcgaggactt gaagagagcc
       61 ttgcagaatg tccaggacct agagcgcctc gccgcccgta ctgtggccgg cacggcatcg
      121 ccgagagatc tggtggcgat caaacagacg ctcctcgcgt tagccgaatt gcaaagctta
      181 ttggccgggg agaacagcga catcttgcgc gcgttgcgcg agcaactttc agttttgccc
      241 gagatcgtcg aactcatcag cgcgtcagtc gtcgatgatc cgcctttttc actgagagat
      301 ggcggtttca tccgccaggg tttcgattcg gagctcgatg atattcgcgg cggccgaagt
      361 caagccaagc aatggatagc gtcatttgaa gtggcggagc ggcgtcgcac cggaatcaat
      421 tcgctcaagg tccgctacaa tcgcgtgttc ggctactata tcgaaataac cagctcgaac
      481 ttaaaagcgg tgcctgagga ttatattcgc aagcagactt tagttaatgg cgaacgctat
      541 atcacgccgg aactaaaaga atacgaaacc agagttctta actcccaaag ccttttagaa
      601 aaattggaaa ccgagttgct cgggcgtgtc cgcgggcaag tcgccgccgc ttatcccgtg
      661 ctgaaaaaat ccagcatggc gctggcttcc atcgacgttt tgctctcgtt ggctgaagtc
      721 gccgaatcgt gccattgggt cgcgccatgg ctagatgacg gattgatgct gacagtgcga
      781 gaaggccgcc atccggtggt ggaagcggcc atcggccgag gcgcttttgt tcccaatgac
      841 tgcgttatcg atccgaacga gcaacagatt ttattgctga cggggcccaa catggcgggc
      901 aagtcaacct acatgcgcca gatcgctttg atcgcggtgc tagcgcagat gggaagcttt
      961 gtcccggcgc gtgaagctcg gatcggcctg gtggatcgtg tgtttactcg aatcggcgct
     1021 agcgattctc tagcccgcgg cgaatcgacc tttatgatcg agatgaagga aaccgctgcc
     1081 atcctggagc atgctacgca gcgcagttta atcctcttgg atgaagtcgg ccgaggcact
     1141 agcacgttcg acgggatctc cattgcctgg gctgtcgcag aatcgctgca cgagcgcacc
     1201 gagcgccccc ggacattatt tgccacccat tatcatgagc tcaccgatct ggcgcatacc
     1261 aaggaacgaa tcagaaatta taacttcgcc gtcaaagagt ggcaggggga cattattttt
     1321 ttaagaaacc tggtggaagg cgcggctagc cgcagctatg gcattcacgt tgcccgcttg
     1381 gccggactgc ccgcggcgct aattgaccat gccaaagcca tcctcgcgca actcgagggc
     1441 ggcggggacg aatgtcgcgg ccgcctagca tctatgaggc catctgccgg tgagcagccc
     1501 ttgcagatgg cactgtttgc aagcgccgag gatcggtttc gcgaacaatt gcgcagtctc
     1561 gacgtttcaa cgatcactcc ggtggaagcg ctcaatattc tttacaaact caccgaagag
     1621 gctaaggaat gattctcatc gcatgaggtg ccggatgacg ataagaaacc tgcgttacat
     1681 tgcgattgca acggctattg tcgctttgct cgggatgtct ttggttcatt tcgattcttt
     1741 cgccgcggct cagagcaata agaaagcccg ggcggcgtcg ccgggtaaat caaccgaagt
     1801 tgaccctccg tcgtcgcgcg acggcgggca gtcgagcagc gatgaattga agcccgcttc
     1861 gcagctttcg accgaccctg cggacaagag cttgatcagt tcagtgcgct tcatctccac
     1921 ggcccgctac actcgcgtgc tgctcgatct ctccaaagag gctaaatatg aaattcaccg
     1981 gctggaggga gacaagtcga aagggctggc ggcgcgaact tacgtcgaca tctcgggggc
     2041 gagactggga accaattcaa aagaaccgct cgccgtgggt gaccggcttt tgcgccagat
     2101 acgcgtcggc cagtacacgg acaatgtggt tcgagtggtt ttggacttag ccgatgatac
     2161 cggccgttat gatatttcat tacttccaga tctttatcga ctggtcatcc atatccagcc
     2221 tgagcgcaca ggtgagacgg tcaaaccagg tgccgataaa ccggtcatca gagcaactgc
     2281 aaaaactaaa cagccggcga agactaccgt gtccggcatc cgcaaaatcg tcttggatcc
     2341 tggccacggc ggtaaggatc cgggcgccat cggtgtcggc ggggttgctg aaaaagattt
     2401 ggttctatcc atcgccaaga aattggcggt caaggtcaaa cgggagatgg gcattgaggt
     2461 catcttgacc cgcaaggacg atcgctacgt tccgctggaa gatcgcaccg ccaccgccaa
     2521 tgccgaggac gccgatctgt ttgtctcgct tcacatgaac gcaagtccaa acctggaagc
     2581 cagaggtttg gaaacctact atctcgacaa caccagcgat gaagcgtcca tccggcttgc
     2641 ggctcgggaa aacagcactt cgcgaaaaaa tatctccgac ctgcagttca tcctgagtga
     2701 catgatgcag aatgtgaagc ttgaggattc catcagtctg gcgcatcacc tccagggcgc
     2761 ggtggtgagc ggcatgtcgc gcaaaatgag cgatgttaaa gacctgggtg ttaagaaagc
     2821 attgttttac gtgctggtcg gggcccggat gccgagcgtt ttagtggaaa tgttttttat
     2881 caccaatcgt cttgaaggcc cggcgatgag tgatgaaggc catcaggacg cgatggtcga
     2941 ttcgctgctc gagggcatcc gtaaatatac tcaaggttcg gtggcgacga agacgttgta
     3001 gaggtaaact attacgtgga cttcacctca ttacctgact ctctgcttga aacgaccaag
     3061 gacctcagcg acttgcctgg gtttacccgt cgctatgtgc agaaagcgcg taccgtcttg
     3121 ttcgaaaagc atcgggccgg cgccggcggt ctggaaatcg ctgctgctta ttcaaaaatg
     3181 atggacgagc tgatttgcca tctgttcggc ggcatcagca aggactttca gagccgttac
     3241 ccgaccggaa atccgcaatg cgcgattatc gcgcagggag gctatggtcg aagcgagctc
     3301 aatccgcact cggatattga tcttttgttt atttactcgt ggaaagtctc gccgttcgtg
     3361 gaggcggtca ccgagaggct cctttatacg ctatgggatg ccgggcttca ggtgggtcat
     3421 gcgattcgca gcatcagcga atcgatgcgc ctggcaagca cggatatgaa agtcaggacg
     3481 gcgctactcg attgcaggta cgtctgcggc gaccaaaccc tctacaatga atttgaaaaa
     3541 gcggtccaga gccggctggc cagaaaacgg gtcagcggtt ttgtcggcga aaagctcgcc
     3601 gagaatgacg ccaggcacgc cggatacggc ggttcggttt accttttgga gccggacgtc
     3661 aaggagagcg agggagggtt gcgggacgtt cataccgccc gttggatggc gcgcgtcaaa
     3721 atgcaggtca aagatctcga tgagctggtt ggcaaaggca tcatcagtgc ggcggatgtc
     3781 gtaaaactga aggcctccca cgatttcctc ttgcgcgtcc gcaatgagct gcatttttcc
     3841 accggtaagc accaagacca attgactttt gaagaacagg agaaggtgag ccaggcgctg
     3901 gggtttcaag gtgaaggcag cctgaaggcg gtggaagtat tcatgagcca atattatcgg
     3961 cacgcttcag aaatcagccg gctcacgacc ctggtcactc atcgactaac cgatagcagc
     4021 tggccgctca tcggcgggat ggtcggtttg gggcgaacca tccgcgacgg agtccgaatc
     4081 tccagagacc aaatcacgat caccaaaccg gagattctta cgcaggatcg cggcaatctg
     4141 atgcgcatct tcgaagatac ccaaaagcat cgctgctcgc tcagccacga gagccgggag
     4201 ctgctgcgcg agcacctgca tttgatcgat gatcagtttc gagcttcggc ggcggccaac
     4261 ctgccttttt ttcggattct caagtggaaa gaaggtgttt acgacacgct gttcgaaatg
     4321 catcgggccg gagtcctcgg cgccttcgtt cccgaattcg gcaggcttct ttgcatggtt
     4381 ttgcacgatg cctatcacat ctacacggtc gaccaacact cgttgcgttt gattcaagaa
     4441 attgagcggc tcaaagccgg cgagttcaag gaatctttgc cgttgctgac gcagttggcg
     4501 cgagaagccg aaaagattga gctgctttat ctcgggctta tgttacacga tatcggcaaa
     4561 ggctttggcg ggggtcattc cgaacggggc gccgtgatgg tgcgccggat cgccgagcgc
     4621 atgcggctca atgtcgatga cggcgcgctg gtggaatttt tggttcgaca ccatctgctg
     4681 atgacgcaca cggcgtttcg ccgcgatctg gaagacgaga aaaccatctg tgacttcgct
     4741 caaacgatgg ggagcgttaa caatctgaag atgctgtacc tgctgaccta tgccgatgtg
     4801 agaggggtcg gtcccgacgt ttggaacccg tggaaggcat cgctcttggg cgaactctac
     4861 gtgaagacgc tgacggtgtt ggaagatatg gagaagggcg agtttcagcg tcaagatatt
     4921 cgcgccgctt tggggcgcat ccaaactcgc gtgcgcgaag aattgttggc gacctcctca
     4981 ccggaagaag ccgattgctt tatccgggtg atgccggagc gttacttttt gtcggtgacc
     5041 gaaggcgaga taccggcgca attcgctctg atgcaggaat acaaaggccg cggcgcggcg
     5101 gtttccgtcg agcactttcc ggaaaaggac tgcagcactg tagccatctg caccaccgac
     5161 cgtcccgggc tatttgcgtc gattgccggg gtgatggcgg cgatgagatt ggatattctc
     5221 aatgctcgga tcttcacggc cagcgatggc cgcatcctcg acgtttttcg agtctcgcac
     5281 ggcgggcgct ccgaactcgc catggccgag cagcgctgga gccgctttag ctcgaccttg
     5341 gaagcggtgc tggagggaaa catcgacgtt gaacaactgg tggaaaaatc gcaatcgacg
     5401 cttttgcgaa aacgcatgcc caaagtgctg acctccatcg agatcgacaa tgaagcctca
     5461 gctcattata cggtcgtgga agtatacacc gccgatcgcc tcggcgtttt gttcaggata
     5521 acctatacgc tgcaccagct tggcctttcg attcatgtgg caaagatctc gaccaacgtc
     5581 gaccaagtgg ccgacgtatt ttttgtcaca actgagcgag gcgacaaagt cgaagactcc
     5641 gcccgcattg aagcgattcg gcagacactc cacgctagcc tcgttccaga tgatgaaaga
     5701 atcgctgcac ccctacattg attcatttct gacgatgatc agtgtggaga aagggcttgc
     5761 caacaacacg gtgcaggcct atagccgcga tctctccgga ctggcggagt tcgtcctcgg
     5821 acagaagata acccgctggg atgccgtgga ggcgattcat cttcgtttgt ttgtgtccac
     5881 tttgcgcgcc cgccatttga gcagtcgctc tatcgcccgg cagattgtca ccgtaaggcg
     5941 tttctatcgt tttctggaaa ctgaaggatt ggtgagtaat cctcccgttc cggaatttca
     6001 tctaagcgct accgcgcgga agttgccgca cgcgcttaac gggggcgata tccgctctct
     6061 cttgaatcaa ccggatccca atcaaatcct cggggcgcgt gatcaagcga tgcttgaact
     6121 tctctatgca acgggtctcc gagtatcgga gttggtgcta ttaaagctcc aacaggtcga
     6181 tttccaaggc gactacctaa cggtcaaagg caagggatcc aaaatgcggg cagtgccgtt
     6241 tggcaaatgg gcgcgagaaa aacttcttcg ctacattcaa gaaacccgac cgcgtttgct
     6301 gaagggcaaa tcgagcgctt ttctatttct caatcgctcc ggtcaagcgc tgagccgcca
     6361 gggattctgg aagataattc ggggttatgc catggctgcc ggcatcgaca agaaagtttc
     6421 gccccacacc ttgcgccatt ctttcgccac ccatctgctg gcaggcggcg ctgatctccg
     6481 ctcggtgcaa acgatgctcg gccatgctga tatatcgacg acgcagatct atactcacgt
     6541 ggatgccaat cgcctcaaac aggcccacca gcgctatcat ccgcgcgaag ggggcgtgca
     6601 gcgtaaaccg aaaccggcgc cggattaggc acaatcctcc acgtaccgga ctcgaaatgt
     6661 tgtcgtttta gcttgtgctt taagaagctt tgccgtagtt ttttaaactg tttctcgaat
     6721 gatgtagagc cttggaagaa accgtaagac agattgcgat ctgggcgctg ccggtattgc
     6781 tggcgatcgt gtttcatgag gttgcgcatg gctgggtggc ttaccacctg ggcgattcga
     6841 ctgccgcgcg gatgggccgc ctcactttaa atccgcttgc ccatgtcgat ttgttcggca
     6901 ctattctgat tcctctgctg ctgattcttt caaatgcgcc atttctgttc ggctacgcca
     6961 agccggtgcc ggtaaatttt caagccctgc gcaatcccaa acgagacatg atctgggtgg
     7021 cgcttgccgg tccggtgacc aattttcttc tggcctttat ttgcgtcttg ctgctcaagt
     7081 ttctcgtgcc catgatcggc aagagctctc cggttggaat gtccgcatcg cttgtcgatt
     7141 tttttgtgcc gatcgttttt atgctcgaaa gaggaatctt gatcaacatc gtgctggccg
     7201 tctttaacct gctgccgctg ccgcctctag acggcgggcg cgtgttggtg ggtttgctgc
     7261 ccgagccttt ttctacgctc ctggcgcgta tcgaaccctt tggctttctt attctactgg
     7321 ttcttctgat gacccgttcg ttgagctgga tcatcggacc tccgctcgat tttttgctaa
     7381 aagttttcct cgaattgatt taggattgga ctatatggaa accatcgtta gcggcgccag
     7441 acctaccggg cgtttgcacc tcggccacct gcacggcgcg ctcaggaact ggattcgctt
     7501 gcaagaaaat tatcgatgct atttctttgt cgccgactgg catgcgctca cgaccgatta
     7561 cgccagccct caaggaatca aacaaagcac cattgaaatg gtcatggact ggcttagcgt
     7621 cgggctcgat ccggcgcgct ccgttctgtt tcgccagtcc cgggtgaaag aacatgccga
     7681 gttgcacttg atttactcga tgattacccc ggtgccgtgg ctcgaacgca atcctacgta
     7741 caaagaacag atcaaggaac tcaccggcaa agacctttcg acgtatggat ttcttggcta
     7801 tccggtactg caggcggcgg atatcacgat ctataaggcc aacaaggttc cggtcggcgt
     7861 agatcaggcg ccgcacgtcg agttgacccg ggaaattgtc cggcgtttca atcaacttta
     7921 tcgttcgata tttcccgaac ccgaggtatt gctcaccgag acgcagaagc tgccgggttt
     7981 ggatggccga aaaatgagca agagttacgg caatgcggtt tttctctccg acccggccga
     8041 ggaaatcgac cgcaagctca gccgtatgat gaccgacccg gcgcgggtca agcgcaccga
     8101 tccgggggag ccggagaagt gtccggcatt tcagctgcac aagatctact gcacgcctga
     8161 agaaatcgaa tttgtcagca ccggatgccg caccgccggc atcggatgtc tcgaatgcaa
     8221 aaagattatg atcaaacatg taatcgaaga tcttgcgcca ttcagggaaa agcgcgcgca
     8281 actggagaaa acccctgaag tggtcgaaga agtgttgcag gccggccatc aagcggcgca
     8341 gaagaaggcg agtgaaacca tgagcgaagt gcgggaaaca ttgggattgt gagcacccga
     8401 gttcagctgg aaatattcga aggtccctta gatttgttgc ttcatttgat taaaaagaat
     8461 gaggtgagca tcaccgacat tcccatcgcc ggtatcaccg agcaatactt ggccacgctg
     8521 gagctgatgg aaacgctgag tctggacgtc gccggcgaat ttttggtgat ggccgctacc
     8581 ctgattcata tcaaatcgcg aatgcttctt ccggcaggcg ccgatgagga ggatgaggac
     8641 gagggcgatg acccgcgcga cgaactggtg cgccggctat tggaatacca gcgcttcaag
     8701 gatgccgcgg atcaactcga acagcgggag atattgagcc gcgacgtgtt tgtccgttca
     8761 gcgacgccgg ttgaagaagc cgctgcgccg actttccgcg aattgtcggt tttcgagcta
     8821 ttaaatgccc tgcggcgagt catcgatcgt ttgccgaagg atgtgtttca ccaggtggaa
     8881 ctcgacaaag tcacggtgcg cgagaagatg actctgctgc tggataaact gcgcgcccag
     8941 ggccgggttt tgtttgagga actgttcaat gaggccaaga cgcgcatgga tgtggtggtg
     9001 acgtttctgg cgatgttgga gctggtcaaa gtgcgcgccg taagaatctt ccaggaagag
     9061 ttgacgggac cgatcattat cgaagccgcc gcctccatgg aacaagccgc ggaactggca
     9121 aatgctgtgg aagccgagga agagcaacat ggaacgtgaa gatgtgaaat ccattctcga
     9181 aagcctgctg tttgtcgccg atggtccttt gacggtgcaa cgtttaaccg aagtcataga
     9241 aggcgccggc aaagacgata ttcgctctat tctcgacgag atggacaacg agttgcaaga
     9301 caatcggcgc ggcatccgtc tggtcgaagt cgccggcgga taccagttgc gcaccgccaa
     9361 ggcgaatgcg gattgggtta aaaaatttct cggcggccgg ccggcgcgaa tgggccgggc
     9421 gacgctggag actttggcga tcgtcgccta tcgccagccc atcaccaagg ctgagatcga
     9481 agcgatccgg ggcgtcgatg tcgacggcgt gatcacaacc ctgctggagc gcaatttggt
     9541 ccgcgccgta gcgcgcaagg atgtgccggg caggcccttt ctatacggga caacgccgga
     9601 gtttttggag ctgttcaatc tcaaggatct ggcgcaactg ccgaccttga aagagatgga
     9661 agagatgacg ttgccggaga ttccgggcga ccaggtttca atggaacaga tcgaagacgc
     9721 ggatcagccg caagaagccg agcagcccca agaagccgag caggagaagc aggattaagc
     9781 cgccatggaa cgcttgcaga aaattctcgc gcgcgcgggt ctcgcttcgc ggcgcgaagc
     9841 ggaacgttgg attctccaag gccgggttac tgtcaacggc accgtggtgc gcaaaatggg
     9901 cactcaggcc gagccttcca gagatagcat ccgcgtcgac ggcaaacgga tcaggcttgc
     9961 cacagaaccg gtttatctgg cgctgaacaa acccgcgggc ttcatcacca ccctcaacga
    10021 tccgcaacag cgccccgata tcactcagtt tctagaaccg ctcggagaaa agcaaagagt
    10081 ctttccggtc ggacgcctgg atttcaactc ctcgggactg atcctgctga ccaatgacgg
    10141 cgagctggcg cagcgcctga tgcacccgcg ctttggcgtc aagaagctct atcacgtcaa
    10201 gctcagtgcg tgtccgactg aaatagagtt aggccggctg cgcaaaggca ttcgcctgga
    10261 agacggcatg acggcgccgg cgcgggtgcg tgtgctcgag aaattaaaga agaacgcctg
    10321 ggtggaaata gaaattcacg aaggccgcaa tcgcgaagtc cgacgcatgt tcgaaaccct
    10381 cgggtatttt gttgaaaaat tgatccgcgt gcgcatcggc aatgtcgaat taggcagcct
    10441 cgcccccggg gaaattcgcc cgttgatgcg gacagaaatc atcaagctaa agcgcgccgt
    10501 tggtcttcaa gataggcggt cagagcccga gaggcctgag agaagaaggc gcgcgccgtc
    10561 tcgtcgttga ctttgccctt agcggtggag tgataatgct tgcggttcgt ttagcgtgcg
    10621 ggcgccgaaa gggcgcgttc cacctcacgt ggaagcatca gagggacgaa ataactgcgg
    10681 ggtagaggta gtcctcgccc gattcgtcaa taaccctgaa gtgcttatcc ttggcggcac
    10741 gcgtattggg aaggatctgg taaatcttcc ggatctcaag gtcttcagcc ccttcgttcc
    10801 gaatgcagat cgcaaactgt tcttct
//
LOCUS       DASUVS010000067         6902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_897135, whole genome shotgun sequence.
ACCESSION   DASUVS010000067 DASUVS010000000
VERSION     DASUVS010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897135"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1389
                     /locus_tag="VF182_03940"
     CDS             199..1389
                     /locus_tag="VF182_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX6436255.1"
                     /translation="MKHSTQRILTTHVGSLARADALLPLLRLKEQGQPYDREELARQV
                     RAAVSDVVRQQVEAGIDIVTDGEQGKSGFFTYVIERFNGFERKPPSPGKEMRPRSAGR
                     EYLAFPDYYAWSERIAAPFGGRSGRGGRDVDICTAPVSYKGHAAVQTDIANLKAAVAG
                     RPHEDVFVPAIASSYVASTFANEYYRSLEEYEQAVSEALREEYQAIVAAGFILQIDDP
                     RLVTYYMMHPELTVEDCRKWAEKRVEAINYSLRGIAPEKVRFHTCYSIDVGPRIHEMD
                     LKDIVDIILKINAGAYSFEAANPRHEHEYHVFERVKLPAGKILIPGVISHTTNLVEHP
                     ELVAERIVRFAKLVGRENVIAGADCGFAASALRFNDTHPSVAWLKFAALAEGARLATK
                     QLWN"
     gene            complement(1529..2527)
                     /locus_tag="VF182_03945"
     CDS             complement(1529..2527)
                     /locus_tag="VF182_03945"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436256.1"
                     /translation="MFCPHSLSGTKQLPILALSVLLVIVSALPSFAADKIRVAFSAVS
                     PTQGVLWVADVGGLLIKNGISAEIVYTRAAIETLVAGEVDFGQMTGSLMSSARLQGAD
                     PVMIAGVQDILEDRLVARPNIKAMEDLRGKRIGVFRFGSASHLRLIYVLPRYGLSNRD
                     VTLLQVGDTPERLIALAGGSIDAALISPPDHLEAQRAGMKILLNLRDLNIAYQGSGLV
                     TTQRVLVRKRDLARRFLKSYVEAIHLVKTNPEISKKAFAKYRQTKDEKRIDDAYQTLR
                     ETVKTKPYPSIEGFKTIIEDASERIPAAKTANPKDFIDVSLLEELDKSGFIDALYR"
     gene            complement(2814..3686)
                     /locus_tag="VF182_03950"
     CDS             complement(2814..3686)
                     /locus_tag="VF182_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436257.1"
                     /translation="MNLEKYIVKARPGVLGGKHEEKRPLRQLSALPVKRYVFINRDSH
                     PDADIYVAIHEAKDLPSPVPDYQVPHCHNTDEFYYFIGDHPDLTGLEGQIIFEGKVHK
                     IVSPACVYIPTGTVHEYKVTAGNGTVTVLFRNRGYTHEDKPFDVSKGERDFARYAGYV
                     FKPEVRSTTEIKYHTDAAPGTRYVFVDGKLKPEANFYTVVRSVTGVQPTQANYVDMHR
                     HNCDTQHIAIGTGPELTGLKIEFQVRDDKMVVESPVGVNIPAGTPHSQRIIEGAGHFF
                     NFVPKSFYNDGLMS"
     gene            complement(3720..4757)
                     /locus_tag="VF182_03955"
     CDS             complement(3720..4757)
                     /locus_tag="VF182_03955"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6436258.1"
                     /translation="MNMQKAVLIILLLLFGPSKISAQSDFFKGKQMKIVVGASAGAAS
                     DLYARVVASHLPKQIPGKPDIIVQNMPGGGSLTAANYVYSVAKPDGLTLGAVTAPIYF
                     AQLLGRKEVQFDWAKFTWIGTPEENDELLFVRSDQPYKNLFDLRKAAEPLRCGASGVG
                     STGFYIPKLIEEIFGARINVVTGYPGAADVDLAVEKNEVHCRGTTISAFFGREPGRTW
                     AKNGFVRFLVQTGDKRNHRLPDTPTMWELMDQEKGTDATKRLARVVLGPGAFGRPILA
                     TPGIPADRVTLLREAYLNMLKDPEFVAEAKKRQWEIAPVSGERLEALAKEVVNQPPDV
                     IERVKKVMGES"
     gene            complement(4761..5678)
                     /locus_tag="VF182_03960"
     CDS             complement(4761..5678)
                     /locus_tag="VF182_03960"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEX6436259.1"
                     /translation="MSPSKKLRQMLGRKNKVLAVLGAPNAFHAKIMEANGIEACFVGT
                     SITGGNYTGQPDLGILSMTECVEFAKWIARAVKIPVILDGDTGHGGIMAVRRLVRECI
                     DAGLAGLRLDDQPLDQKRRTGSAGISIINREDAIMRYRVAVDAKNEIDGDFVIMAQCY
                     ARNAVNGGINELLRRLRLYESEGRVDWVQFESPHSVAEIKRARRVVKGPFSAMKGQLP
                     RALTLEEHAAFGLNAAWYTFLPDLVLKTAAWSFFQEFKDKDMTAWDEFAAAHSGTPFT
                     GGGKLPWDATGMTELSKLEQNYAIKSKRR"
     gene            complement(5675..6823)
                     /locus_tag="VF182_03965"
     CDS             complement(5675..6823)
                     /locus_tag="VF182_03965"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6436260.1"
                     /translation="MARNGYKIFDADTHVRPDAELLEPYMASSARDKLAPYEKYRAKN
                     KDGAVTYLMGERSYKRRLGLSGDAAPQKDEYMSGYKRHQHGKPDPLCERDPAVRIKDM
                     DIEGIDVHLMLPSGWFGCWTTIDDVALECAVYEAYHRWMADYCAVFPERLKGVILVSA
                     RDVTTSLAELRRAAKESWPLAIFVYAPYQFPLDHPELEPIWQAAAEYDLSIALHTFTV
                     MPPYAPGGLDSWDNLWLQRSAAHPWCGQRNMAAIIGAGIMDRYPNIRVGTLEAGHGWL
                     PVWVSRLEEHTKLCPEALPPLEQTIRSYVTGGRYFQSIEVSEGAEITRSVIELLGPDI
                     LMFGSDYPHGESWFPVSVDTVMDWNLKPEHLRKLLWDNAVKYYRRYSA"
BASE COUNT         1450 a         2021 c         1907 g         1524 t
ORIGIN      
        1 agccagggga gtactagcgc tttatgcaag tcagcaaagt ctgatcccgg agcacagctt
       61 cgagcactct cgactgctct ggcaaccatt ctctattaca agttgacact tcctagccca
      121 tgcggctaag tttcacggca atgtcggcgc tttagtgtcc gggttcagcg tcagccaacc
      181 aacagaaggg gaaatgccat gaagcacagc acgcaaagaa ttctcaccac ccacgtgggg
      241 agcctggccc gagccgacgc cctgttgccg cttcttcgac tcaaggaaca aggtcagcct
      301 tacgaccgcg aagagcttgc gcggcaggtc cgtgcggcgg tcagcgacgt ggtgcggcag
      361 caggtcgaag cgggcatcga tattgtcact gacggcgagc agggtaaatc gggtttcttc
      421 acctacgtca tcgaacgatt caacggcttc gaacgtaagc cgccgtctcc gggcaaagag
      481 atgagacccc gctcggcggg cagggagtat ctcgcttttc ccgactacta tgcgtggtcc
      541 gagcgcatcg ccgcaccgtt cggcggtcgc agcggtcgcg gtggccgtga tgtcgatatc
      601 tgcaccgcac ccgtcagcta caagggccat gcggcggtcc agacagacat cgcaaacctc
      661 aaagcggcag tcgcaggccg cccgcacgag gacgtgtttg tgccggcgat cgcttcttcg
      721 tatgtcgcgt ctacttttgc caacgaatac taccgcagcc tcgaggagta cgagcaggca
      781 gtgtccgagg cgctgcgcga agaatatcag gcgattgtcg ccgctggatt tattcttcag
      841 atcgacgatc cgcgcctggt tacttattac atgatgcacc cggagttaac cgtcgaagac
      901 tgccggaagt gggcggaaaa gcgtgtcgaa gcgatcaact actcgctccg cggcatcgcg
      961 cccgagaagg tccgctttca tacctgctat tcgatcgatg tcggtccgcg catccacgaa
     1021 atggacttga aggacatcgt cgacatcatt ctgaaaatta acgccggcgc ctattcgttc
     1081 gaagcggcca acccgcggca cgaacatgaa taccatgtct ttgagcgggt caagctgccc
     1141 gcgggaaaaa tcctcatacc cggtgtgatc agccatacga caaatttagt ggaacacccg
     1201 gaattggtcg ctgaacggat cgtgcggttc gccaagctcg tcggacgcga aaacgtgatc
     1261 gccggcgccg actgcggctt tgccgcttcg gctctcaggt tcaacgatac ccatcccagc
     1321 gtggcatggc taaagtttgc cgcgttggcg gaaggcgccc gattagcgac caaacaatta
     1381 tggaattgag ctaaagaagt ttccttcgac aaaggggtag ggttcaactt ctttcaaccg
     1441 acttcgaatc taaacctgca accacgccgt aaggcgttta cggcttgaac gtagcgcagc
     1501 gcttgagcgg cttgaaccga ttctggatct atcgatagag cgcatcaata aatccagatt
     1561 tgtccaactc ttccagcaaa ctcacgtcga taaaatcctt cgggttcgcc gtcttggccg
     1621 cgggaatccg ctcgcttgca tcttcaatga tggtcttgaa tccctcgatc gacggataag
     1681 gcttggtttt gacggtctcg cgcagagtct ggtaggcgtc atcgatgcgc ttctcatctt
     1741 tggtttgccg atatttggca aaggcctttt tggagatctc gggattggtc ttcaccaggt
     1801 ggatcgcctc gacataggat ttgagaaaac gtcttgcgag atcgcgcttg cggaccagca
     1861 cccgctgggt cgtgaccagc ccactacctt gatacgcaat attcaaatca cgcagattga
     1921 gtaaaatctt catgcctgcc cgctgcgcct ctaaatgatc tggaggcgag ataagggcgg
     1981 cgtcgatgga accgccggct aaggcaatca gccgttccgg agtgtcgccc acctggagca
     2041 gggtgacgtc cctattactc aagccgtaac ggggcagcac gtagattaaa cgtaagtgcg
     2101 aggctgaacc aaagcggaaa acgccgatcc gttttcccct gagatcttcc attgctttga
     2161 tattgggacg cgccaccagc cggtcctcga ggatgtcttg cacgccggcg atcatcactg
     2221 gatcggctcc ttgcaacctc gcagaggaca tgagggagcc cgtcatctgg ccaaaatcga
     2281 cctcacccgc caccaaggtt tcgatcgcgg cgcgtgtgta aacgatttct gcgctgatgc
     2341 cgttctttat taaaagcccg ccgacgtcgg cgacccacag caccccttgg gtcggcgaaa
     2401 ccgccgagaa tgccactctg atcttgtccg cggcaaaact tggcagagct gaaacaatca
     2461 ccagcagaac gctgagtgca agaatcggca attgtttagt gccggataga gaatgcgggc
     2521 agaacatcag ggaccccggt aagttgtctg tgtgttcagc gcagcctgga gattttccga
     2581 caggtgtgtt tcgtcggacg atagtagagt cagtgagcag cgtcaagcac aaatcagaat
     2641 ggtcgttcaa gcgaacgctc cgatcggagc cgtcctccag aatcgtggaa atatcgccgg
     2701 agcggcgttg gtgatccctc ggaaaacccg gttcagtcac ggttccgctg ccttcctcag
     2761 cgcttgcctg gcgctcctga aacgcgctac caaacagaac ggcgagtgcg acttcaactc
     2821 atcaacccgt cattgtaaaa gcttttcggc acaaaattga agaaatggcc cgccccctcg
     2881 atgatgcgct gcgagtgtgg cgtgcctgcg ggaatgttca ctccgaccgg actctcgacg
     2941 accattttat catcgcggac ctggaactcg attttgagtc cggtcaattc aggtccggtg
     3001 ccgatcgcaa tgtgctgcgt gtcgcagttg tgccggtgca tatcgacgta gttcgcttgg
     3061 gtcggttgca cgccggtgac gctgcgcacc acagtgtaaa agttggcctc cggcttcagc
     3121 ttgccgtcga caaaaacata acgcgtccct ggagccgcat cggtatggta cttgatctcc
     3181 gtcgtcgacc gcacttccgg cttaaaaacg taaccggcgt atcgtgcaaa gtcgcgttcg
     3241 cctttactga catcgaacgg cttatcctca tgagtgtaac cgcggttgcg aaataagacc
     3301 gtcacagtgc cgttgccggc agtcaccttg tactcgtgca ctgtccccgt gggaatatag
     3361 acacacgcgg gcgaaacgat cttgtgcact ttcccttcaa agatgatctg accttcgagc
     3421 cccgtgagat ccgggtgatc accgatgaag tagtagaact cgtcggtatt gtgacaatgc
     3481 ggcacctgat agtccggaac cggcgatggc aaatctttag cttcgtggat cgccacgtag
     3541 atatcggcat cggggtgcga atcgcggtta ataaatacgt aacgctttac cggcagcgcg
     3601 ctcaattggc gcagcggccg tttctcctcg tgcttgccgc cgagcacacc gggccgcgct
     3661 ttgacaatgt atttttctaa gttcataaat ctcctcttag gattgcgagc gtatcgatgt
     3721 tacgactcgc ccatgacttt tttcacccgc tcgatgacgt ccggcggctg attgacgact
     3781 tctttggcca aagcctccag gcgctcgccg ctgaccggtg cgatctccca ttgtcgtttc
     3841 tttgcttcgg cgacaaattc aggatctttc agcatgttga gataggcttc gcggagcagc
     3901 gttacacgat cagcagggat gcctggggtc gccaggatcg gccggccgaa agcgcctggt
     3961 ccaagaacga ctcgcgccag ccgtttggtc gcgtccgtgc ccttttcctg gtccatcaac
     4021 tcccacatcg tcggcgtgtc gggcaagcgg tggttgcgct tatcgcccgt ttgcaccagg
     4081 aatctgacaa agccgttctt ggcccaggtt cttccgggct cgcgcccgaa gaaagcgctg
     4141 atggtcgtcc cccggcagtg gacttcgttt ttctctacgg ctaaatccac gtccgcagct
     4201 cccggatagc cggtcaccac attgatcctg gcgccgaaga tttcctcgat caacttgggt
     4261 atgtaaaatc ccgtgctgcc gacacccgaa gcgccgcagc gcagcggttc ggcggccttg
     4321 cgcaaatcga acaggttttt gtacggctga tcggagcgaa caaacaacag ctcgtcgttt
     4381 tcttccggcg tgccgatcca ggtgaacttg gcccaatcga actgaacttc ctttctgcca
     4441 agaagctgcg cgaaataaat cggcgctgtc accgcgccca atgtaaggcc gtccggcttg
     4501 gccacgctat agacatagtt cgcagccgtc agcgatccac cacccggcat attctgcacg
     4561 atgatgtccg gtttgccggg aatctgtttt ggcaggtgac tcgcgaccac gcgggcatac
     4621 agatcgctcg ctgcgccggc ggaggcgccg acgacgattt tcatttgctt gcctttgaaa
     4681 aaatcgctct gggcgctgat tttggagggg ccaaacaaca acaataagat aatcaacact
     4741 gccttttgca tgttcattat ctatctccgt ttcgatttga tcgcatagtt ttgttctaac
     4801 ttgctcaact cggtcatgcc ggtcgcgtcc cagggcaact ttccgccgcc ggtaaacggc
     4861 gtcccgctat gcgccgccgc gaattcatcc catgcagtca tgtctttgtc tttgaattcc
     4921 tgaaaaaaag accaggccgc cgttttcaga accaagtcgg gcagaaaggt gtaccatgcg
     4981 gcattgagcc cgaacgctgc atgctcttcg agcgtgagcg cgcgcggtag ctgccctttc
     5041 atcgccgaga acggcccttt gacgactctg cgggcgcgct tgatctcggc cacagagtga
     5101 ggcgactcga actgcaccca atcgacgcgg ccttcgcttt cgtaaaggcg caaccgccgc
     5161 aacaattcgt tgatgccgcc gttaacggcg ttgcgcgcgt agcactgcgc cataatgacg
     5221 aagtcgccat cgatttcatt cttggcgtcc accgctacac gatagcgcat gatcgcatct
     5281 tcgcggttga tgatggaaat gcccgccgat ccggtccgtc gtttctgatc caacggctgg
     5341 tcgtcaagcc gtaagccggc cagtcccgca tcgatacact cgcgcaccag gcggcgcacc
     5401 gccatgatgc cgccgtgacc ggtatcgccg tcgagaatga ccgggatctt caccgcgcgg
     5461 gcgatccact tggcaaactc cacacattcg gtcatcgaca gaatgccgag gtccggttga
     5521 cccgtatagt tcccgcccgt gatgctcgtg ccgacgaagc acgcttcaat gccgttggcc
     5581 tccatgatct tggcgtgaaa ggcgttgggg gcgccgagta ccgccaggac tttgttcttg
     5641 cggccgagca tctggcgtaa tttcttgctc gggctcatgc cgaataccgc cggtaatatt
     5701 tcactgcgtt gtcccacaaa agtttgcgca gatgctcggg tttcagattc cagtccatca
     5761 ccgtatcgac tgaaaccgga aaccagctct cgccgtgggg gtagtcggaa ccgaacatga
     5821 gaatatccgg ccctaaaagc tcgatcacgc tgcgggtgat ttccgcgcct tcggagactt
     5881 cgatactctg gaaataacga ccgccggtga catagctccg aatcgtttgt tcgagcggcg
     5941 gcaacgcttc gggacagagc ttggtgtgct cttccagccg gctcacccac acgggcagcc
     6001 agccatgtcc cgcttccaag gtgcccacgc gaatattcgg atagcggtcc atgatcccgg
     6061 caccgataat cgcggccata ttgcgctgcc cacaccaggg gtgcgcggcc gaccgctgca
     6121 gccatagatt atcccaggaa tcgagaccgc ctggcgcgta gggaggcatg actgtgaagg
     6181 tgtgtagagc gatgctcaag tcgtattccg ccgcggcttg ccagatcggt tcgagctcgg
     6241 gatgatcgag cgggaactga tacggcgcat agacaaatat ggcaagcggc cagctctcct
     6301 tcgcggcgcg acgcaattcg gcgagcgaag ttgtcacgtc gcgcgcggat accaggatga
     6361 ctcccttcaa gcgttcggga aaaaccgcgc aataatcggc catccagcgg tgataggctt
     6421 cgtaaaccgc gcactcgagc gcaacgtcat cgatggtggt ccagcaaccg aaccaacccg
     6481 aaggcagcat gagatggaca tcgatcccct cgatgtccat gtcctttatg cgaacggcgg
     6541 ggtcgcgctc acagagcgga tcgggcttgc cgtgctgatg tcgcttatag ccgctcatgt
     6601 attcgtcttt ctgcggggcc gcgtcgccgc tcaaacccaa gcgtcgctta tacgagcgct
     6661 cgcccatcaa ataggtcacg gcgccgtctt tattcttggc ccgatacttt tcataaggcg
     6721 ccagcttatc gcgagcggaa ctcgccatat acggttccag cagttccgca tcgggccgaa
     6781 catgtgtgtc cgcatcgaag atcttatagc cattgcgtgc catggttggt tcctcgtttg
     6841 gttagcgcta tcacattcgc tagccgtaaa tctggtagac tgtcaagctc tttttgtcag
     6901 gg
//
LOCUS       DASUVS010000068         9867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1115973, whole genome shotgun sequence.
ACCESSION   DASUVS010000068 DASUVS010000000
VERSION     DASUVS010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9867
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115973"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1323)
                     /locus_tag="VF182_03970"
     CDS             complement(25..1323)
                     /locus_tag="VF182_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX6436261.1"
                     /translation="MNEVVGLDFGTTNSAISIAKLDGTVELAKFKVDNHISPTFRSIL
                     YFDAENLDKDNSPRAVAGPEAITSYLKGRTHGRLIQSMKSYLASHLFSQTNIFNYTFS
                     LEDLIAMIVRELRVAAESQFGKLGKSVVVGRPAHFSGAKEDTDDSFALNRLRSAIEAA
                     GFEQVTFEFEPVAAAYKYQMQLDHEELVLIADFGGGTSDFSLVQLRPCNRSYEGNDSV
                     IIGTDGVGIAGDTFDSKMMRHVVAPRLGLNSRYRSPFGKILAVPFWLYEHLEKWHYLS
                     FLKTKRTMEQLKELSFQALEPDKINALIHVVENDRGYQLYRSVENTKCELSQNAAASF
                     SFEDVPVQITELMTRHDFEGWIAPETQQIADCIDRLLKQCHLSPKDVDTVFMTGGSSF
                     VPAIRSLFEQRFGGAARLRAGEELTSVATGLAVSALRRFS"
     gene            complement(1440..1706)
                     /locus_tag="VF182_03975"
     CDS             complement(1440..1706)
                     /locus_tag="VF182_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6436262.1"
                     /translation="MVTVFILPHGETAWNKLGRVMGRSQVPLGADGIQQIQKIAPLVA
                     TLELDGTYTSLLRGRYNRQCRLGGKPDLQERRIERNRLWRLGIW"
     gene            complement(1779..2948)
                     /gene="moeB"
                     /locus_tag="VF182_03980"
     CDS             complement(1779..2948)
                     /gene="moeB"
                     /locus_tag="VF182_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEX6436263.1"
                     /translation="MAKTFQEIMADARKEIPEVTAQQVNDLLTNNGKGHVLLDVREKD
                     EWREGHLEGALSLPRGFLEIKVETAVPEKNTPIIAYCAGGTRSLLAGKILKEMGYQNV
                     TSMSGGYAAWKNAGYRWVQDFQYTPEQLVRYSRHFLLPEVGEEGQAKLLGAKILLVGA
                     GGLGSPAAYYLAAAGVGTLGIIDNDVVDISNLQRQILHANDRVGTPKVESAKKTLEGL
                     NPDVKVIPYQAKLTSQNIMEILKDYDFVLDGCDNFPTRYLVNDACVLMGKPNVHGSIF
                     QFEGQATVFYPGKGPCYRCLYPEPPPAELAPSCAEAGVLGVLPGLIGVIEALEAIKLI
                     LDKGDSLVGRLLTFNTLTMEINTLRLRRDPNCPMCGDKPTIKELIDYEEFCSLRH"
     gene            complement(3067..3918)
                     /locus_tag="VF182_03985"
     CDS             complement(3067..3918)
                     /locus_tag="VF182_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6436264.1"
                     /translation="MTPARIEIRKFRRFDKVAGASAVFIMIVLLSGFAVPWISPYGSG
                     ELQANSLLEGPSWTHPMGTDALGRDLLTRLLYGARVSMTVGIGTALIALIIGTTYGLI
                     SGFKGGSLDDFMMRLVDIFYGLPDMLIFILLSLLFGKSIGGLLLALGLVTWVRFARIT
                     RGQVLQAKEFVYVEGARAMGAPRRRILLRHILPNILGPILVTLTFSIPAAILAESTLS
                     FIGLGINDPYSGWGTSWGTLAQDGWRAMRAYPHIIFFPAAAIVLTILAFNTLGNALRD
                     LLDPRTR"
     gene            complement(3926..4924)
                     /locus_tag="VF182_03990"
     CDS             complement(3926..4924)
                     /locus_tag="VF182_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6436265.1"
                     /translation="MKKDRPTRGKLYLKRSDIPQPELIAIFMAPFLLKRIFHGVVVLW
                     VVATLTFVLLRLTPGGPFDRERKLPPQVVANLEAKYHLDESFLKQYFRYLTGIVRGDL
                     GPSYKYLDRGVNEIVRDTLPTSALLGALAIAFALAASIPIGLFAAYRRGSWIDRLLMI
                     SASIGISVPHFILGALLIWALALQLNWFHAGRWDALSSAVLPTITLGAAPAAYLSALL
                     RSSLIETLGEDFIRTARAKGIGETGVLLKHALSQSLIPMLTVMGPLAATLLTGSFVVE
                     YVFAIPGMGRFFITAVTDRDYPLIMGVTLIYTALLVSANLIVDLLYGFVDPRIRSQ"
     gene            complement(4896..6365)
                     /locus_tag="VF182_03995"
     CDS             complement(4896..6365)
                     /locus_tag="VF182_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436266.1"
                     /translation="MSEVKFIDTTLRDGQASLWAENMRTGMMLAVAERIDNAGFQAIE
                     LIASSHIKKCVRELKEDPFERIRLVADKIRKTPLRAIGAERISAFELTPDSIVELWFE
                     RLVANGIKQLRISDPANDPTGWRKSVNRAKRNGLGTVLNLIFSVSPKHTDEYYRRKAG
                     EAAALKPDYICLKDPGGLLTPERTAALIRVIQKQAPGFPIELHVHCTTGLGPLCALEA
                     VKLGVQVIDTSVPPLANASSNPSIFNVAQNARALGYSPVIDEEELKSVARDLTFFAKQ
                     EEMPIGVPVEYDYSQYVHQVPGGMISNFHHQLRNLGLIHLLPKILEEAVQVRADLGYP
                     IMVTPFSQFVGSQAAINVIKGERYGQVTDQVIQYATGLWGEEAAAEVDPNIKDKLLSG
                     PRAKEFANWVPPQPTLEEVRDKFGGSSVNDDQLLMYYVVGKDDVDALRHVGGPKQYIS
                     ARTPLLMLLQQLSRRECTQIYIRTKEMTLALEKRPADAR"
     gene            complement(6453..7817)
                     /gene="accC"
                     /locus_tag="VF182_04000"
     CDS             complement(6453..7817)
                     /gene="accC"
                     /locus_tag="VF182_04000"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019961904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX6436267.1"
                     /translation="MSVSRVLVANRGEIAVRIIKACQSLGIETVAAVSEADRESLAAK
                     LSNRSVCIGPPRSIDSYLKTATLVAAAQATGCDALHPGYGFLSERAELAQACADNKLT
                     FVGPSVENITIMGDKLQARQVASSSGVPTVPGSDHARTPEEAAQLAEKVGYPILLKAS
                     AGGGGRGIKLVSNAGEMEMTFKTAAAEAQAAFGNNTLYMERYVGDARHIEVQILGDHL
                     GNVVHLGERDCSLQRRHQKIIEEAPAYALDAEVRAKICEAAATLARSIGYRNAGTIEF
                     IYDNETQEFFFLEMNTRIQVEHPVTEMITGVDLVAQQLLIAGGQPLSLKQTDITFTGH
                     AIECRINAESPQHGFRPCPGRISNWQAPDGAGVRVDSHCYSGYFVPPYYDSLLAKLIV
                     HGANRAEAINKTRQALASFHVAGIDTGIPFLQTVIDDQDYRSGHVNTRWLEKKLAEYS
                     EVQG"
     gene            complement(7814..8326)
                     /gene="accB"
                     /locus_tag="VF182_04005"
     CDS             complement(7814..8326)
                     /gene="accB"
                     /locus_tag="VF182_04005"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX6436268.1"
                     /translation="MELSEDDVLHILKLIDESKFDYFQLEVGELKITVSKGEPIPLNS
                     SPQQVATAPVPSPAPMSAKPAAAPATAAAAPAAQTAPASVEARAAAEGLLTVTAPLLG
                     TFYVAPEPGAPPFTQVGATVTEDTTVGLIEVMKVFNSVRAGVKGTIVEAVAQNGQFVE
                     YGQTLFLVKP"
     gene            complement(8653..>9867)
                     /locus_tag="VF182_04010"
     CDS             complement(8653..>9867)
                     /locus_tag="VF182_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6436269.1"
                     /translation="SEYAYILFDLVNAQEFHEGKLADAGEVGVRALDDVTLEISLRHP
                     ASYFLSLTTFEVTFPQRQDIVERFQSRWTDPEHIVTNGPFTLASWKHENEIQLRANPK
                     YFLGKPAMDQVTMLMVSEKTTALAMYEQGQLDFIDNRSIPTLEKKRIAKQRGFHHVPQ
                     LRGYYYGFATHKKPFNDPRVRKAFAMAIDRDTFPKILQGGEKPASSWIPPGMLAHNSK
                     IGVHFNPPEARRLLREAGYPDGKGFPQVVLAYNTEEDHKLVAEAVQSMWQRNLGVIVR
                     LDNQEWKVYLKNLQTDPPHIFRLGWGADYPDPDNFMKLFTANSGNNNTRWKNPRYDQV
                     IELAARELDNNKRVKLYDEAQKILVETDLPIVPLFWTSESTLLNPRFTGLEFNSMARI
                     DLRHVRPAEAIR"
BASE COUNT         2243 a         2669 c         2690 g         2265 t
ORIGIN      
        1 tgaacagacc gcatcaacaa agaattacga aaatctgcgt agagcactaa cggccagtcc
       61 cgttgcaacg gatgtgagtt cttcacctgc ccgcagccgg gcggcgcctc caaatctctg
      121 ttcaaaaagg cttcttattg cagggacaaa cgaggagccc cctgtcataa aaactgtgtc
      181 gacatcctta ggcgatagat gacactgctt aagtagacga tcgatgcagt cagcaatttg
      241 ctgagtctct ggtgcgatcc atccttcaaa gtcgtgacgt gtcatcagct cggtaatctg
      301 tactgggacg tcctcgaaac tgaatgaagc ggcggcgttt tgagaaagct cacactttgt
      361 attctccacc gacctgtaaa gctgatatcc acgatcattc tcaacaacat ggattaacgc
      421 atttatcttg tctggttcta acgcttgaaa acttagttct ttaagctgtt ccatagttcg
      481 cttagtcttc aagaaggaaa gatagtgcca cttttccaag tgttcgtaga gccaaaatgg
      541 caccgccagt attttgccaa atggagatcg ataacgtgaa tttagtccga gtcttggtgc
      601 gaccacatgg cgcatcattt tgctatcaaa tgtatcaccg gcaatgccga ccccatctgt
      661 tcctatgata acgctgtcgt ttccttcgta gctccgatta caaggccgaa gctgcacaag
      721 agaaaaatcg ctagttccac caccaaaatc agcgatcagc actagttcct catggtcaag
      781 ctgcatctga tacttataag cagccgccac cggctcgaac tcaaacgtaa cttgttcaaa
      841 acccgctgcc tcgattgccg accgaagccg attaagagcg aagctatcat cagtgtcttc
      901 tttagctcca ctgaagtgcg caggtcttcc tactaccacg cttttgccga gcttcccgaa
      961 ctgactctcc gctgcaactc tgagttctcg cacaatcatg gcgataaggt cttccaacga
     1021 aaaagtatag ttgaagatat ttgtctgact aaaaagatga cttgccagat aggacttcat
     1081 ggattgaatt aagcgaccat gagttctacc tttgagataa gatgttatag cctcaggtcc
     1141 ggcgaccgca cgtggactgt tgtctttgtc aagattctcg gcatcaaaat aaagaataga
     1201 tctaaaggtt ggtgaaatgt ggttgtcaac tttgaatttc gccaattcaa cagttccatc
     1261 gagctttgcg atcgaaattg cactgttagt tgttccaaaa tcaagtccca ctacttcatt
     1321 catatcgagc gatcatactt cctaatttac aggttcttgt gttttcaact cacgactatc
     1381 ctaaccgaca agttagccgc gcttaacgtt caactcaaaa aagcagtggc cacagccttc
     1441 taccatatgc ccaatcgcca aaggcgattt cgttcaatcc tacgttcttg caaatcgggt
     1501 ttcccgccga ggcgacattg gcggttgtac cgcccgcgga gcaggctggt gtaggtgcca
     1561 tcgagctcca gcgttgcaac cagcggcgca atcttttgta tttgttggat tccatcggca
     1621 ccgagcggca cttggctccg ccccataacc cgtcctaact tgttccaggc ggtttcacca
     1681 tgagggagta taaatacagt tactatttac attaagctcc aaaaaaaagg gccggcttcg
     1741 cgccggcccc tgctgttaaa tttattggaa aactgaactc aatgtcgcaa actgcagaac
     1801 tcttcgtagt cgatcaattc tttgatcgtc ggcttatcgc cgcacatcgg gcagttggga
     1861 tcgcgccgca gcctcagcgt attgatctcc atcgtcaaag tattgaaggt cagcagccga
     1921 ccgaccaagc tgtcgccttt gtcgagaatg agcttgatcg cttccagcgc ttcgatgaca
     1981 ccgatcaatc ccggtagtac gcccaacact cccgcttcgg cgcaactcgg cgccaactcg
     2041 gccggtggcg gttccgggta caagcaccgg taacaaggcc ctttgcccgg atagaaaacc
     2101 gttgcctggc cttcgaactg aaagatgctg ccatgaacgt ttggcttgcc catcaaaacg
     2161 caagcgtcgt tgaccagata ccgggttggg aaattatcgc aaccgtcaag aacgaaatcg
     2221 tagtctttaa ggatctccat gatattctgt gaggtgagct tcgcctgata ggggatcacc
     2281 ttaacgtcgg gattgagacc ttcaagcgtc tttttcgccg attcgacctt gggtgtgccg
     2341 acccgatcgt tggcgtgcag tatctggcgc tgcaaattgg aaatatcgac gacgtcgttg
     2401 tcgatgatgc ccaaagtacc gactcctgcc gccgccagat aatacgccgc cggcgaacct
     2461 aaaccgccgg cgccgaccaa taagattttt gctccgagga gtttggcttg cccttcctca
     2521 ccgacttccg gcagcagaaa atgccggctg tagcgaacca gttgctccgg cgtatattga
     2581 aaatcttgca cccatctata gccggcgttc ttccacgcgg cgtatcctcc ggacatcgag
     2641 gtaacgtttt gataacccat ttccttcaga attttgcctg ccagaagcga acgggttccg
     2701 ccggcgcagt atgcaatgat cggcgtgttc ttctctggaa ctgcggtttc aactttgatc
     2761 tcgagaaagc cccgtggcag cgaaagcgct ccttcgagat gcccctcccg ccattcatct
     2821 ttttctcgga catcgagcag aacgtggcct ttaccgttgt tggtcaaaag gtcgtttacc
     2881 tgttgcgctg tgacctccgg gatctccttg cgcgcgtcgg ccataatctc ttgaaacgtc
     2941 ttggccatgg gttcctcctt cttggaccta gaaggtctaa gttaagcctg gctaaaaaca
     3001 atgtcaagga agggaggaga aactggactc atctacaaac cacagccggt tcaaagcggg
     3061 ccaggttcat cttgtcctcg gatcgagcag atcgcgcaac gcattaccca atgtattgaa
     3121 tgccaggatc gtcagaacaa ttgcggcggc aggaaaaaaa ataatgtgcg ggtaggcgcg
     3181 catcgcgcgc cagccgtcct gcgcaagggt tccccagcta gtgccccagc cgctatacgg
     3241 atcgttgatt cccaaaccga taaaactcaa ggtggactcc gccaaaattg ccgccgggat
     3301 actgaaggtc aaggtgacga ggatcggccc cagaatattg ggcaaaatat ggcgcagcag
     3361 aatcctgcgc cgcggcgcgc ccatggcgcg cgcgccctcg acgtagacga attccttggc
     3421 ctgcagcact tgccctcggg taatgcgcgc gaagcgcacc caggttacca aaccgagcgc
     3481 caataagagc ccgccgatgc tcttgccgaa cagcaacgat aacaaaataa agatcaacat
     3541 gtcgggcaat ccatagaaga tgtcgacaag ccgcatcata aaatcgtcga gactaccgcc
     3601 tttgaatccc gagatcagtc cataggttgt gccgatgatc aaggcgatca gcgcggttcc
     3661 aattcccacc gtcatcgata ctcgcgcgcc gtacaagagc cgggttaaca ggtcgcgacc
     3721 gagcgcgtcg gtgcccatcg ggtgagtcca cgacggtcct tcgagaaggc tgtttgcttg
     3781 tagctcgcca gaaccgtacg gcgagatcca gggcacggca aatccgctga gcaaaacaat
     3841 catgataaag acagcgctgg cgccggctac tttatcaaag cggcggaatt ttcgaatttc
     3901 gattctagct ggggtcatgc gcgcctcatt ggctgcggat tcgcggatcg acaaatccgt
     3961 aaagcaggtc gacgatgaga ttagcgctga ccaagagagc cgtatagatc agcgtcactc
     4021 ccatgatcaa tggataatct cgatcggtca ctgcggtgat gaagaaacgg cccatgccgg
     4081 gaatcgcaaa gacgtactcg accacgaaag atccggttag aagcgtcgcc gccagcggtc
     4141 ccatgaccgt gagcatggga atcagcgact ggctgagcgc atgcttgagt agcacaccgg
     4201 tctcgccgat gcccttagcg cgagccgtgc ggataaaatc ttcccctagg gtttcaatca
     4261 atgaagagcg cagcagggcc gacagatacg cggcgggcgc ggcgcccagg gtaatagtgg
     4321 gcaggaccgc gctgctaaga gcgtcccagc gaccggcgtg aaaccagttg agctggagag
     4381 ccagcgccca tatcaatagt gcgccgagaa taaagtgagg cacggaaatt ccgatggaag
     4441 ccgagatcat cagcagtcga tcgatccatg agccgcgccg ataggcggca aacaagccga
     4501 tgggaatcga ggcggcaagc gcgaacgcga tggcgagcgc tcccaggagg gcggaggtcg
     4561 gcaaagtatc gcggacaatc tcgttgacgc cgcgatccaa atacttatac gagggtccga
     4621 gatcgccgcg gacgatgccc gtgagatagc ggaaatactg cttcaaaaaa gattcgtcca
     4681 gatgatactt ggcttcgaga ttggcaacga cttgcggtgg aagtttgcgc tctcggtcaa
     4741 aaggcccgcc cggcgtcaag cgtaaaagga caaatgtcag ggtggcgacg acccaaagta
     4801 cgacaacgcc gtggaaaatt cgtttcagca gaaacggcgc catgaatatt gcgatcagtt
     4861 cgggctgcgg aatatcgctt cgcttgaggt agagtttacc gcgcgtcggc cggtcttttt
     4921 tcaagcgcca gggtcatctc tttggttcgt atatagatct gagtgcattc gcgccggcta
     4981 agttgttgca gtagcatgag cagaggtgtt ctagcgctga tgtactgttt cgggccgccg
     5041 acatggcgca acgcatcgac atcgtctttg ccgacgacat aatacatcag caactggtca
     5101 tcgtttacgc tcgagccgcc gaatttgtcg cgaacttcct cgagggtcgg ttggggcggc
     5161 acccaattgg caaattcttt ggcgcgcggg ccgctcagga gtttgtcttt gatattggga
     5221 tcgacttcgg cggctgcttc ttcaccccat aagccagtcg catattgaat tacttgatcg
     5281 gtgacctggc cgtaacgttc tcccttgatc acgttgatgg cggcctggct gccgacaaac
     5341 tgtgaaaacg gcgtgaccat gatgggataa ccgagatcgg cgcgaacctg caccgcctct
     5401 tcgagaatct ttggcaacag atggatcaat ccaagattgc gcagctgatg atgaaagtta
     5461 gagatcatgc cgccgggcac ctgatgaaca tactgggaat agtcgtattc caccggcacg
     5521 ccaatgggca tctcttcctg cttggcgaaa aacgtcagat cacgggccac cgacttgagc
     5581 tcttcttcat cgatcaccgg cgagtaaccc agagcgcggg cattctgggc gacattgaaa
     5641 atggatggat tcgaagaggc gttggccagg ggcggaacgg atgtgtcgat cacctgcacg
     5701 cctagtttca ccgcctcgag cgcgcacagc ggccccaagc cggtggtgca gtgaacatgg
     5761 agctcgatag gaaaaccggg cgcttgcttt tgaatcactc ggatcagagc ggcagtgcgc
     5821 tcaggggtca gcaatccgcc gggatctttt aaacagatgt agtctggttt cagcgccgcg
     5881 gcttcgcctg ccttacgccg gtagtattca tcggtgtgtt tcggtgaaac tgaaaagatt
     5941 aaattcagca ccgtgcccag gccgttgcgc ttggcgcggt tgacggattt gcgccaaccc
     6001 gtcggatcat tggccggatc ggagattcgt agctgcttga tgccgttggc gaccaggcgc
     6061 tcaaaccaga gctcaacgat cgaatccggg gtcaactcga aggcgctgat gcgttcggcg
     6121 ccaatggcgc gcaacggagt tttcctgatt ttatcggcaa ccaggcgaat gcgctcgaag
     6181 ggatcttcct tgagctcgcg cacgcatttt ttgatgtggc tggacgcgat cagctcaatc
     6241 gcttgaaagc cggcattgtc gatccgttcc gctaccgcca acatcatgcc ggtgcgcatg
     6301 ttctccgccc acaggctcgc ctgcccgtcg cgcagagtgg tgtcgatgaa tttcacttca
     6361 ctcatatcta ttaagaaagt tatgaaacat gaaagctgaa aacggaaatg ggaagttctg
     6421 gtcggattct tctcataatt cagctttcat cttcatcctt gcacttcaga gtattctgcc
     6481 aatttttttt caagccagcg ggtattcaca tgaccgctgc gataatcctg gtcgtcgatg
     6541 acggtttgca gaaacgggat tccagtgtcg atgccggcga catggaagga agccagggct
     6601 tgccgggttt tattgattgc ttcagcccga ttggcgccgt ggacgatcag cttggccaat
     6661 aacgaatcat aatagggcgg cacgaagtaa ccggagtagc agtggctgtc gacccggacg
     6721 cccgcgccat ccggagcctg ccaattggag atccgcccgg gacaggggcg gaagccgtgt
     6781 tgcggcgatt cggcgttgat gcggcattcg atggcatggc cggtgaacgt aatatccgtt
     6841 tgttttaagg aaagcggttg gccgccggcg atcaagagtt gttgcgcgac cagatcgacg
     6901 ccggtgatca tttcagtgac cggatgctca acctggattc tggtattcat ctcgaggaag
     6961 aaaaattcct gcgtttcatt atcgtagatg aattcgatgg tgccggcatt gcgatagccg
     7021 atgctgcgag ccaaagtcgc cgcggcttca caaatcttcg cccgcacctc ggcgtccaga
     7081 gcataggccg gcgcctcttc gataatcttt tgatgacggc gctgcagtga gcagtcacgt
     7141 tcacccaggt gaacgacgtt gccgagatgg tcgcccaata tctggacttc gatatgccgt
     7201 gcatcgccaa cgtagcgttc catgtaaagc gtgttgttgc caaacgctgc ttgcgcctct
     7261 gccgcggcgg ttttgaaggt catctccatt tccccggcat tggagaccag cttgatgccc
     7321 cggccgccgc cgccggcgga tgccttgagc aagatgggat agccgacttt ctcggccagc
     7381 tgcgcagctt cctctggagt tctggcatgg tcggatcccg gcacggtagg cactccggag
     7441 gagctggcga cttgcctggc ttgaagcttg tcgcccatga tcgtaatgtt ttcgacactg
     7501 ggcccgacaa atgtcagttt gttatcggcg caggcttgcg cgagctcggc gcgctcggag
     7561 agaaacccgt agcctggatg aagcgcgtcg caaccggtag cctgggcggc cgccaccaag
     7621 gttgcggttt ttaagtaact gtcgatcgaa cgcggcgggc cgatgcagac cgaacgattg
     7681 gataattttg ccgcgagact ttcgcgatcc gcttcggaaa cagcggcaac ggtttcgatc
     7741 cctaaagact ggcaggcctt gatgatacgg acggcgattt cgccgcgatt ggcaacgaga
     7801 acccggctga cgctcatggt ttgacgagaa agagcgtctg accgtattcg acgaactgac
     7861 cattttgagc gacagcttcg acaatggttc ctttgacgcc ggcgcgaacg ctattaaaaa
     7921 ctttcatcac ttcgatgagc cccaccgttg tatcttcggt gacagtggcg ccgacttgag
     7981 tgaacggcgg cgcgccgggc tccggcgcta cataaaaggt cccgagaagc ggggccgtta
     8041 cggtgagcag gccttcggcg gcggcgcgcg cttccacgct ggcgggcgca gtttgtgccg
     8101 ctggcgcggc agccgctgtt gccggagctg cggctggttt cgcgctcatc ggggccggcg
     8161 acggcaccgg cgccgtagcg acctgctgcg gcgaggagtt cagcgggatt ggttctcctt
     8221 tgcttacggt aattttgagc tcgccgactt caagctggaa gtaatcgaac ttggattcat
     8281 cgattagttt caatatatgc agcacatcgt cttcgctgag ttccataccg tctacctttc
     8341 tcggtggtgt tttattgcac tgcggaagga ttccggaagc gctggttcta atggcaccta
     8401 catgatttta tggcgcatgg caacaatagt cgctgcgcgc cggatccaat cgttccagac
     8461 gcttcaatcg ctgcgctcgt tccgccaaac attcatcgac attgtaacgc cacaatccgt
     8521 tgcggcttca gagattttcg tgatcgtgtc cgtgaagaac gtgatcgaga attccgaccg
     8581 aacacgacca cgtaacacga acacgaacgg aaacgagtcg tacgcgcgac ctcgaagcac
     8641 tctccgtagc tgctaacgga tggcctccgc cggccgcacg tggcgcagat cgatgcgcgc
     8701 catggaattg aactcgaggc cggtgaagcg gggattgagc agggtacttt cgctggtcca
     8761 gaagagcggc acgatcggca gatcggtctc gaccagaatc ttttgcgcct cgtcgtaaag
     8821 tttcacccgt ttgttattat ccagctcgcg ggcagcgagc tcgatcactt gatcatagcg
     8881 cggattcttc caacgagtat tattgttgcc gctgttggcg gtaaatagct tcatgaaatt
     8941 gtccgggtcc gggtagtcgg cgccccatcc taaacgaaaa atgtgcggcg gatcggtttg
     9001 cagatttttt aaataaactt tccattcctg attatcgagt ctgacgatca cgcccagatt
     9061 gcgctgccac atgctctgca ccgcctcggc gaccagtttg tgatcttctt cggtattgta
     9121 cgccaacacg acttgaggaa atcctttgcc gtccggatag ccggcctcgc gcagcaggcg
     9181 gcgtgcctcc ggcggattga aatgcacgcc gatctttgag ttgtgcgcca gcatccccgg
     9241 cggaatccaa gaggaagccg gcttttcgcc gccctgaaga attttcggaa aagtgtcgcg
     9301 atcaatggcc atcgcgaatg cttttctgac gcgcggatcg ttaaacggct ttttatgggt
     9361 tgcaaagcca tagtagtagc cgcgcagttg cggcacgtga tgaaaaccgc gttgtttggc
     9421 gatgcgcttt ttttccaacg tcgggatgct gcgattgtcg atgaagtcca actgtccctg
     9481 ctcgtacatc gccaaagccg tggtcttctc gctcaccatc aacatcgtga cttgatccat
     9541 cgccggcttg cccaagaaat atttcggatt ggcgcgcagc tgaatctcgt tctcatgttt
     9601 ccaggaggct agtgtgaagg gaccgttggt gacgatgtgt tccggatcgg tccaacgcga
     9661 ttggaatctt tcaacgatat cctgccgctg cgggaatgtg acttcgaaag tggtgagcga
     9721 gagaaaatac gaagcgggat ggcgtaagct gatttcaaga gtgacgtcat cgagcgcccg
     9781 gacaccgact tcgccggcat cggccagctt gccttcatga aattcctgcg cgttgaccag
     9841 gtcgaaaagg atataggcgt actccga
//
LOCUS       DASUVS010000069        31573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1553200, whole genome shotgun sequence.
ACCESSION   DASUVS010000069 DASUVS010000000
VERSION     DASUVS010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 31573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31573
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553200"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(471..1742)
                     /locus_tag="VF182_04015"
     CDS             complement(471..1742)
                     /locus_tag="VF182_04015"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6436270.1"
                     /translation="MEEQLTNPVEAPTQAIPKRFRFQTFSSLGYLDYRYLWTGTLMMS
                     AGQWVQQVALGWLVYELTGSSVLLGLLNGLRALPFLITGPMAGVAADRMDRRQLMLRT
                     QYVLIGTAFLMAALVGSRWLEVWHIFVFTLITGIGWSFSEPVRQSLIPSVVPKTELAN
                     AIALNSAGFNLMKVLGPAFGGVMIAVFGAAGNFWVQGAAYVVVLFMIQLMHVPPTRAD
                     ALRASALANLKEGFAYVRSTRTVLALMILAYVPRIFAVPYQTLMPVFQKDVLQVGPEG
                     LGMLMAAPGLGAVLAVLTLASLSSRMKRQGLMLITSTMMLGLSLILFSQTTSFPLALI
                     ILVIAGAFQMVFLASTSTMLQLIVPDELRGRVMSLYMLDRGLMPAGALFAGLVAHLIG
                     APLAVTCMGSIVIVLTMLVAWRIPHIRTLES"
     gene            2220..2819
                     /locus_tag="VF182_04020"
     CDS             2220..2819
                     /locus_tag="VF182_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436271.1"
                     /translation="MKKLSKIACLTGVMACLSIQAVVAQTSEKAFDAQWVEYSDHHIS
                     VAFDRIPVADAVQAIREKTGFQFSLPSSADSKFLNLRLERSPLEPAVYSLLSNIGFRN
                     FALIYDEHGRPNRAVVLGAQVDEPAPILADASQAAEPVAQPLNPEERDTLQKELDRWT
                     ELKQEQRGRIEDRLKTLPPSEEREQLVKEYGRRLLGIKK"
     gene            complement(3136..4596)
                     /locus_tag="VF182_04025"
     CDS             complement(3136..4596)
                     /locus_tag="VF182_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEX6436272.1"
                     /translation="MKTPEFEELKSLDLRECATVGDIVSGMRYCAFGARMLGEVANTI
                     RQMIGSNEKQIIIYDGAEESPLGSLLREFVKNKWAQKIVLPSQYAKQKTSGENVIVVG
                     AFSERYADAIYTKAARAIFINPFDMARPGQIKDGYFPDAVFADPRYVIPVIYSALDQW
                     INGRSISVTALIAELNEYGGVATQVSRAAKALQAMVSDDECVRFLTVSGAMTVGKMDL
                     LICDMIEMGFVQAVSSTGALMAHGLVSSIGLKHYKYNPAYDDTALARHKLNRVTDTLE
                     PETNLDTVEKVVGEVIKQFNGVRSISPTLLNKAIGKYLAEHYPKDRGILKSAYLHGVP
                     VFVPAFVDSELGNDLYIHNIRRKRRRKQPIVMDLELDSKLLIELVTKNRRFGIFTIGG
                     GVPRNNVQNVAPLIEIINERLGKIYPERRFTYGVRVCPDRPHFGHLSGCTYSENESWR
                     KAVKDGIYAEVLADATQVWPFLVKYLMERQKPRPNG"
     gene            4980..5729
                     /locus_tag="VF182_04030"
     CDS             4980..5729
                     /locus_tag="VF182_04030"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6436273.1"
                     /translation="MKLSGKVAFITGFGSGLGQAIAVMFAKEGAAVAGTSTTESKGQE
                     TVAMIEKRGGNALFRAGDVGDSEQMQSLIAAAVEQYGGLDIVVNSAGVRTNGSITEIS
                     EQAWDRTLDVNLKGAFVVSRLAIPEMKKRGGGVILHIGARSGMLGQAGRAAYCASKGG
                     LIRLTEAMAMDHAKDKIRVNCICPGPVRTPMVDTSTPEKLARYKTRVPLGRIGEPDDV
                     AYAALYLASDEASYVTGAILPVDAGMRLTGP"
     gene            complement(5765..6391)
                     /locus_tag="VF182_04035"
     CDS             complement(5765..6391)
                     /locus_tag="VF182_04035"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX6436274.1"
                     /translation="MALLGMAAAWRWTPLANQVDIGKITAWAIALRTNPSRVSIILGA
                     YLIGSIISLPITILILATALVFGPLEGIIYSFIGCMIGAGVTYAMGYFLGSDFIRQLI
                     GAKWKALERKITQTGILAVAALRAIPIAPFTVINIISGAFQVPVRDYIFGSLLGLAPG
                     IIVTNLFAHQLQSAIRNPGLGSFVLVAALVVVSILGTIWLRRKFASKD"
     gene            7032..7475
                     /locus_tag="VF182_04040"
     CDS             7032..7475
                     /locus_tag="VF182_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEX6436275.1"
                     /translation="MTLLLRLLLVLTLSSHSAWSGELTAAGLWKTVDDHTGKPRGLIR
                     IVQVSGEYQGKLEKIFPQPGEDPNPKCDKCSGARRDQPVIGMTILWGLTKQGEEYQGG
                     EILDPENGKIYRAKMKLEDGGKTLHVRGFIGISLLGRTQVWMREE"
     gene            7543..7920
                     /locus_tag="VF182_04045"
     CDS             7543..7920
                     /locus_tag="VF182_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HEX6436276.1"
                     /translation="MPEKKTLRRARKAKQEGKAPSTQAGEFVREEIEHVRGGKHGARS
                     TKQAIAIGLSKARREGVKLAPPATGKTSPMTRKKAAADLKAARKKKVTTKKRSRASLK
                     ALRREGRRAASKKSLSRQAKKAR"
     gene            8062..8376
                     /locus_tag="VF182_04050"
     CDS             8062..8376
                     /locus_tag="VF182_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HEX6436277.1"
                     /translation="MKTLVLYSIAAIMYVVGGALMKYSQGITHALPTLGLAALFSAGA
                     LIQARGMRYEQLSSSYIVVLGLEALLAIIVGTLWFGEQLSGRAAAGIAFVVIGIVLLR
                     LQ"
     gene            8463..9590
                     /gene="dnaJ"
                     /locus_tag="VF182_04055"
     CDS             8463..9590
                     /gene="dnaJ"
                     /locus_tag="VF182_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX6436278.1"
                     /translation="MAERNYYQILGVERSASHDEIRKAYRKLARKHHPDINPGNKEAE
                     NKFKEISTAYDVLGDEKKRKLYDEFGEAGLAAGFDADKARSYQQWQQQSAAGAGGGYE
                     FDMDDLSEIFGGLGGLGRRGRSNGQGPMRGEDIESSMDIDFLDAVRGFQTSITLQRPI
                     TCDTCNGSGTRPGSKPIQCPECGGSGSKSVAQGPLQFRQTCPRCRGSGKLPGDPCAVC
                     RGSGRVLRADTIRVNIPPGAEPSKRIRLRGKGEAGVRGGPPGDLYIIPRIRPHPLLTR
                     SGKDLSMDLPITIGEAVRGASVEVPTPMGKLNVKIPAGAQSGQQLRLKGKGVSAHGQS
                     PAGDLYLRLMIRVPQDNKISADVIDRLDQAYAEDVRKDVRL"
     gene            9603..9896
                     /locus_tag="VF182_04060"
     CDS             9603..9896
                     /locus_tag="VF182_04060"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HEX6436279.1"
                     /translation="MARKYFQITEVIKTCKVDKNFVQQLERESVICPVVQRRRKLYPP
                     DQVDRIRIANLLIQEMGVNLEGAEVVLNMREQMIAMQRQFNDVLRLIGHEMKR"
     gene            9952..10626
                     /locus_tag="VF182_04065"
     CDS             9952..10626
                     /locus_tag="VF182_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6436280.1"
                     /translation="MDLELIKSDVVKASHILHREGVAAAFGHLSARIPGTDTFIFPPR
                     MSPALVRADNLLELDVEGNQLSGEGRPNTEFWIHARIYKARPDVQAICHVHPPSCVVL
                     SSLGETIRPLHASGAIFRNDVQVFDRITLIRTRELGDAVASTLGDHGAMLLRGHGVNV
                     ADKDVRRVCVMTLWMEEAANYQLRAMSAGKPRYFTAEELETIYPQVSGEEVSKRVWEY
                     LSSRVN"
     gene            complement(10752..12215)
                     /locus_tag="VF182_04070"
     CDS             complement(10752..12215)
                     /locus_tag="VF182_04070"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEX6436281.1"
                     /translation="MHWHEWGEDPFDLARNQDKPVMLLLTAFWCRFCQRLDEEAFSDN
                     ENLALLNAYFISVRCDNAQRPDIDIRYNRNGWPTIAFMTADGDLLSVTNYLPSEQFGD
                     LLVRIYTAYHEKKDQIRLLHRNAQESGNIRQSMNDPQPDLGAIANIARQILDLADREY
                     GGYGRGQKFIQPQVNDFLLARYEATKESRYLDQVCLTLDRMRESPMRDPEAGGYFRTC
                     SGRDWSRPHREKLLAEQAGVLANCLRAFQNTQLPVYARMAEELIAYLNAHLFDTATGG
                     FFGCEDFLRKEDGERSSDEEFFSILDDSIYIDANAQTVIAYLDAEVILPEPRCRDRAL
                     DALEFLWERCRSRNGAMAHYFDGAAQVAGLLDDQIHMGNALLKAYSTAEDTKYLERAI
                     ALGQFILAQLKNPQGGYYDIEIPGPALAAFRITLIAQNGAAASFFLRLAEATRESRYR
                     DAAVWALAAFTGDFGPYGLDAAGFGRALGEYADLCSS"
     gene            12417..13697
                     /locus_tag="VF182_04075"
     CDS             12417..13697
                     /locus_tag="VF182_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HEX6436282.1"
                     /translation="MQLTLAYHPVSEIRFGSRTRLDDTVLAVDAGELRRLVLEDEALA
                     AVDFDIVRPGDSCRAGPIYDIVEPRAKAPESGADFPGILSAPTTAGLGTTHVLAGSAV
                     SVLAEMSPGEGRSATGRVLEMSGPVVSASDYSSLQHILIIPHTKPELPRQVVLKAYRL
                     AALKVAVQIARCAIDRAPASVRSFPSVGPSLGDRDDLSRVAYAGQIFSRQRKPGVDEP
                     ILYGANTEGMLPVVLHPDEWLDGAVVPSLNSWFGGTETYFYQNHPVVLELYRRHEARE
                     INFVGTIATIAGSDQFDRERHCQAAAHLVKWNLRADGAVLTKYGGGLPHADLAETARL
                     LEGMGIRTAVMVSDSSRDRRVESALLFNFPEVNAIVYNGGNGTQWEVPRVERVIAATP
                     ELAELLAGPMAMEAANIIGVTNQQGASRMRSLVY"
     gene            13739..15025
                     /locus_tag="VF182_04080"
     CDS             13739..15025
                     /locus_tag="VF182_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:14777..14779,aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HEX6436283.1"
                     /translation="MRVVHYLNQFFGGIGAEEHAGAPLQARDGAVGPGRLLEALLGDQ
                     TRIVMTLVCGDNYAVENQQAVTASVVEKMRPLRADLFVAGPCFDAGRYGMAAGALCAA
                     VQAELNIPAITGMNEENPGADLYRDALYIIDSGSNPANMREVLGKMAGLGRKLVQKRA
                     VGLPAEEGYLRRGLLRDQVVKHTAARRLADMLVAKLNGTPFESEMLPVAFQPTPMPAP
                     IKNLSQARVMLITDGGLVPKGNPDKIQGTAATRWGAYSLEGRDDLRGQDYEISHGGYD
                     ARFVQEDPDRLVPLDAMREMEKRGVIGKLHNEFISTSGRVNPLSNTRRLGREMAQKIK
                     RDGVDAVILTSTUGTSTRSGAAITTELEKAGIPVVQITSALPIAKMIGSNRIVLGNGI
                     VHVTGDAKLSPEDEKDLRRRLVQKALDALQSSETQT"
     gene            15174..16037
                     /locus_tag="VF182_04085"
     CDS             15174..16037
                     /locus_tag="VF182_04085"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEX6436284.1"
                     /translation="MEKTANEKRKTLRALLQGPGCSIAPSCNDGIQARLVAWLGFPLV
                     HISGSGQHRALGFADAGMLTLTEMINRAREIVDAVDLPIVSDGETGYGNAVNVVRAMR
                     EFERAGVAAIHIEDQMTPKRAGHEGFDVGLVSKGEFVAKIKAALDTRRDENLVIIARS
                     EAKDSLQERLDRVGACCEAGADAVWLSARTEDEIKAFAKLGKPLVGVPPRQLMTIDRY
                     GELGGRVGCIPTILQVAALHGMRQCLEELKRSGTEARYFKETPGIDDTRKWYTNLGNQ
                     ELKELEAKYSY"
     gene            16088..17443
                     /locus_tag="VF182_04090"
     CDS             16088..17443
                     /locus_tag="VF182_04090"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6436285.1"
                     /translation="MDSVTIVIAEYASISVFEHLTLEAVHAAKQRLIDSVGCAVGAHD
                     CQPAQIGRRLARGQTPGKHAGRVLYYGDRLPVDSAAFINTAMIRHFDFNDRYPGGHPS
                     DCLGALLAMAGAMDIDGKRFLASMIVAYEIFARLSDATMLSRRGWDQGYAIGIATAAG
                     VCNLLKISPAATAHAVEIAAASNLPLRVTRSGELTPWKNAATAYATRNGVFAALLAAE
                     GMSGPANAFEGRNGLFEKVTGPFELAPFPNCGGAYLTPRVQLKYWPIETNGQPAVWAA
                     LELREKAQIHELKEIEVFTSKFTWFEIGSEPEKWDPQTRETADHSLPYIFARTLADGP
                     IKITSFADDAVRDPGLRSLMSKIKVIADDRIEALLPQKTFMRVVATTLDGRQHVVEIA
                     NPLGHPDNPMQDSHVEEKFIALVEPALGKTRCRAALDSWWRLDEATEVSPAMQLLDNN
                     R"
     gene            17577..19013
                     /locus_tag="VF182_04095"
     CDS             17577..19013
                     /locus_tag="VF182_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6436286.1"
                     /translation="MSADQNTFAPAAEGLKTTAAAATFIEQVTAGDLPSEAVRIGTRC
                     LLDGLGLYVAGSQEHSVELLIEEAEHVGGRAEAMLLGRGKTKVPASTAARVLGTAGHA
                     HDWDDSQVSSDPTHVYGLLTHPTIPPLTSAVVMSQRSGNIDGQAFMLAFLAGFEVECK
                     ISEWMLPQHYLRGFHSSGTVGTFGAYATAAKLLGLKGGSLRSGFGIAASFAAGIRCNF
                     GTMTKPLHVGRAAENGVTAALLAARGFTADPDALDGAWGFYAVHGGGVSAEKISQDFG
                     KIWTIVEPGVSVKPYPCGVLTHPTIDLMLKLVTEHEIKPDGIEAVTVYAGTNILKPIR
                     YPIAANHLEAKFSLPAALAMIALARKAGKREFSDEFVGSPAMEAMQRRISTEFDPEIE
                     KRGFDKMRSRIRIDLKDGGKIEGWADERYRGGPENPLSDADLEVKVRSCCEGVLDEKS
                     QTTLIDAAWSIGKIKDAARLMEIINKLP"
     gene            19065..19745
                     /locus_tag="VF182_04100"
     CDS             19065..19745
                     /locus_tag="VF182_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6436287.1"
                     /translation="MIDPIEAIKEKMIMVCKILQKQRVIDGYGHVTARLPDGRILSTP
                     HMPPGKVAVRDLIIIDGQGNKLEGAGEPNGETSMHTSIYKARPDVQSILHYHADELIA
                     VAASGQGIKVLSNAGVHFYRGTPIYDSPLLIRNHQLGDKVAATLSDRSAVLLRGHGGT
                     VVAKNLDQLVRRGLDLVRSAKLQIMASTLGPLKTHSDDECEQMIQGHDGRDAQRRFLD
                     YYISEVVD"
     gene            19986..21773
                     /locus_tag="VF182_04105"
     CDS             19986..21773
                     /locus_tag="VF182_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6436288.1"
                     /translation="MSEASKTDKSAIKKSTQRWQSDVIVDLIKQYDFPYIALNPGASY
                     RGLHDSLVNYGENDPPMLLCNHEKIAVQIAHGYAKATGKPMMAIVHNVVGLLHAPMGI
                     YYAYIDRCPVFIVGATGPMDEKLRRPFIDWIHTAFAQGDVVRGFTKWDYQPGSIHGVP
                     DSFARAYSIMMSEPQGPVYMVYDSAMQESPMKEEMALPKFKVKVPSRVAPEPAVIEEA
                     ADMLMAAENPLLLTEYAARMPIGFDSTVELAETLGASVFDINKRLGFPNQHPLSVSFH
                     GDAFKGVDVVAALDVIDWTRGSHKLNTQTREISVVTAPDCKWIDVGFADIEISKWATD
                     YNKHHNWDLRVLGDSALSLPALTTACRKRIDGNKSMTAKIDERKKAVGERHARQWQKW
                     QEQVKQEWNERPMTESRLAHEVWQVIQNEDWVLTANTLKEWVHKIWNFDRSYRHVGRE
                     LGTGTQIGMSIGVALAHKGSGKLVIDLQPDGDLMFDLGALWMPIKYQIPMLIVMYNNR
                     AYYNDWAHQIHMAHQRGTDPARAYIGMDLEGPPPDFAHIAKGIGWHAEGPIEDPAEVG
                     PAVKRAIAQVKAGKPALVDTIVWRRGENA"
     gene            21895..23682
                     /locus_tag="VF182_04110"
     CDS             21895..23682
                     /locus_tag="VF182_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6436289.1"
                     /translation="MAKPAPKAETIKKSDKRWQSDVIVDMIKRYDFEYIALNPGASYR
                     GLHDSLVNYGENDPPMLLCQHEKIAVQIAHGYARVKGKPMIAIAHNLVGLLHTPMGVY
                     YAYLDRAPVFLIGATGPLAEPKRRPFIDWIHTANVQGEAIRNFVKWDYQPSTVDGVPG
                     AFARAYSVMMSARQGPVYMCYDAGLQEAQLDHDVQMPPPGAIDVPTAPSADPAALEKA
                     ADTLAAANRVAIIADFAARPPHGWNHVVELVETLGASVWDVGSRLNFPTNHPLNLTMD
                     PEGCYKDVDVVLTLDIADFEKPTHTRDISTRTVVSKVQEKATWIDIGFTDIEISKWSM
                     DYARPFYAQQRMTADPVTTVPLLTKLLKARIANSKGLNEKIARRKEAIGKRHAENRAK
                     WSKQAQEHWDAKPMTVPRLALEVWDAIKGEDWVLTTGDLGSWGKKLWDFDRSYRHGGR
                     ELGTGTQIGLSLGVALANKGTGRLVVDLQPDGDLMFDAGALWIAAKYKIPMLIVMFNN
                     RAYYNDWNHQIVMAKNRGTDPSRAHIGMDLYDPDPDFAGLARSMGWYAEGPFETGDDL
                     KPALKRAIEQVKQGKPALIDAVCDRRNHG"
     gene            23877..24527
                     /locus_tag="VF182_04115"
     CDS             23877..24527
                     /locus_tag="VF182_04115"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX6436290.1"
                     /translation="MKGFLVGFAWLMGVTLTGFAALGADSSPAYNWTGFYLGVNGGGG
                     WGHSRHDFEAAGTTTGDYRIRGATVGGTLGANLQAGGLLLGLEGDMNWSDIGGSDSCP
                     NVNFTCRTRNKWLATARGRVGYALDRFLPYITTGAAFGDVHTEIPDFGSSREAEVGWT
                     VGGGLEAGIVRGLSAKMEYLYVDLGKFNCGFSCTATGVDDVKFNAHIVRIGLNYRF"
     gene            24885..25148
                     /locus_tag="VF182_04120"
     CDS             24885..25148
                     /locus_tag="VF182_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436291.1"
                     /translation="MLKLSLTAMAIAGALFKATGFLFVSLMNLVFRPYGGAYLALLIS
                     IYPGYDPVSVPLGLVVGTFYSLLSGALAGLLLGWLYNFFAPKI"
     gene            25528..26205
                     /locus_tag="VF182_04125"
     CDS             25528..26205
                     /locus_tag="VF182_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6436292.1"
                     /translation="MHILVVEDETKVASFIRRGLEASSYSVDVEHDGAAGLKRLLEHD
                     YDLIILDVMLPRLDGLSLMKEIRQRQINIPVLLLTARVTIADKVMGLDLGADDYLTKP
                     FIFEELLARVRALLRRSTSAPALLAAADLRLDPATREVTRGGKRIDLTPKEFALLEFL
                     LRRHDQVLSRAVIAQHVWGVNYDTFTNVIDVYVNYLRNKIDNGFQIKLIHSVRGVGYV
                     LKADSSV"
     gene            26202..27632
                     /locus_tag="VF182_04130"
     CDS             26202..27632
                     /locus_tag="VF182_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6436293.1"
                     /translation="MIGHPSTLRGRLTLWYTGILTATLALLGAISVVLLDRGLRNNID
                     DSLNSVARTIAESVRRPASFGDIEDSLQGLLGPELAERFFQLLDPFGRLDPRVAPPGR
                     SRLPLSPTAHQNAERGQPTWETVVIRPGSNKPFRLLTLPVVRNGATVNIVQVAMSLEN
                     VDAARSRFLLILLILAPVALAASALGGWFLARRALKPVDVMVDAARRIEAEDLSNRLP
                     ALPSDDELGRLATVLNDMLARLEQSFGAVRRFSADAAHELRTPLTIIKGEIEVALRSS
                     QDPAEIRQALRSCLEEVDRLNALMEDLLLMARMDAKALSAPASLVNLTDVVQEAAPAL
                     TELAIRAGNHCTVVATAPLWVHGYESLLFRLVFNLAENAIKYTPRRGTIDVTLEQQGS
                     AAVLQVKDNGAGIAAEAQQHIFDRFYRGDPSREGNGTGLGLALVRSIAELHHGQISVS
                     STLGQGSCFRVVLPRTAAPLSSAPQD"
     gene            27736..29187
                     /locus_tag="VF182_04135"
     CDS             27736..29187
                     /locus_tag="VF182_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX6436294.1"
                     /translation="MVSKKKIIATAWAAALAGGGFAIGGAIQNSSVPVQAGATEPGTA
                     SRASLPAFADLAERVSPAVVNIKVTAVARAGVQDDSFGEEFPFPGFRSPIPQQPREFR
                     REGSGSGFIIRKDGLILTNNHVVEKALEITVTLSGKQQYKAKILGRDPKTDLAVIKID
                     PKAALITASLGNSDELRVGDWVMAIGNPFGLSNTVTSGIVSAKGRTIGAGPYDDFIQT
                     DASINPGNSGGPLFNMAGEVVGINTAIFSQGGGNIGIGFAIPVNLVKNLVPELETKGT
                     VTRGWLGVTVQPMTPELARSFGLDKEYGALVGDVMAQSPAEKAGIKRGDVIVGFNGTK
                     IGDTATLPALVASTPVGKTVPVEILRDGKTHTLKVAIAKLDDQIATLEPEQEKGEWGL
                     TLQNIKPEERTAMNLTGNEGVLVASVVPGSPAARANINAGDVILQVNRASVTSVQQMK
                     DEIAKSKGDKPLLLLLRRADGGTSYAALSRNVG"
     gene            29419..29802
                     /locus_tag="VF182_04140"
     CDS             29419..29802
                     /locus_tag="VF182_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436295.1"
                     /translation="MELKIKLLEAFEESYYLIVIAQGKVGSAALKRLFAEVEKITEPL
                     PDCKVFIDLEEARLNVRREAVHAIVTQLELHSKSRLGKMVVVSSELDRCGRLQHVRDL
                     LCSRGFRVAVFDDTTSAAEWLSEGT"
     gene            30102..30533
                     /locus_tag="VF182_04145"
     CDS             30102..30533
                     /locus_tag="VF182_04145"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX6436296.1"
                     /translation="MDWLLQTTDSLAYVFLRFGLAVTFFFHGTQHAFGWHGGKGMKEQ
                     IANWHNKYHIPVAIGMFGTFVELFTVFAMALGFLVRPAAFFLAIFIATAMFKSHWGHG
                     FFLQQKGGGSGIEYTLALLLMALAVLFGGAGAFSIDRMIIR"
     gene            30641..>31573
                     /locus_tag="VF182_04150"
     CDS             30641..>31573
                     /locus_tag="VF182_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX6436297.1"
                     /translation="MEIGGRKSDLSRRDLVTTTATADDTDESFGPGTLGARYGNVPQS
                     WDFEADVVVIGSGAAGLPAAIRAVESGASVIVVEANYDVGGHAILSGGNVPLGGGTSA
                     QRKFGIEDSPDVVFSDLTDWSIVQPNGWPDYRYNDREVMRAFADHCAPTYEFLVANGV
                     EFKDIAPDNQGAHTTGNSAPRENHAFWKKGAGPESPNARPGTGLIRPLEASARKKGVK
                     FLLNYKMTSLIRETQGARRVVGMTAYYRPRIMPGESAPLKSFRSDGNINSTKPNVNIR
                     AKKAIVVATGGMTSNLHFRRMFDPRLTDVLQVAGE"
BASE COUNT         7164 a         8534 c         9158 g         6717 t
ORIGIN      
        1 gggcgtcgac agccgcttcc cactgtccct ccgcacagag gccctgtaca ccggcatcct
       61 cgtatgcctg caggaccgct tcaatgcatg tgtctcgaat acgtttggcg agatcttgaa
      121 cgtccatata gctcctagag aacgaacgtt tagattatgc cgactattgc gtcggcttca
      181 atattatgac ccgcaactgc ggtatcaatc acgagcgtgt cagcaaattg atgaaaaata
      241 acttggagtc ggcgcatgga tatcgtccct cgcgtattct tgtccgcttc tttgtcgcga
      301 tctcttcgcg aggcgagcag cgacaacctt gcgacccttt ccaggttgaa cgacttggcc
      361 accggcatcc cttacataat ctacctgcgc tgcggttagc gcgtacgcgt aggaatttat
      421 gagctgcttc aaccggtcgc tgccctggca aggaaatgag catgactcgt ctagctctca
      481 agggttctga tatgcgggat gcgccatgct actagcatcg tcaaaacgat gacgatcgat
      541 cccatacaag tcaccgctaa cggcgcgcct atcaggtggg cgacaagtcc cgcaaacaat
      601 gcgcctgccg gcattagtcc gcgatccagc atgtacaggc tcattacgcg gccccgtagc
      661 tcgtccggga ctatgagttg cagcatcgtg ctggtactcg caagaaagac catctgaaat
      721 gcgccggcaa tgactagaat aatcagcgcc agtggaaaag atgtcgtctg ggaaaataag
      781 ataagcgata aaccgagcat catcgtgctt gtgatgagca tcaatccctg ccgtttcatc
      841 ctgctcgata acgatgcaag cgtcagaacc gcgagcacgg ctcccagacc cggcgcagcc
      901 atcagcatac cgagaccttc cgggcccact tgcaacacat ctttttggaa caccggcatc
      961 aaagtttgat atggcacggc aaagatgcga ggaacatagg ccaggatcat cagcgccagc
     1021 acagtccgcg tagacctcac gtaagcgaag ccctctttga gattggccaa tgcggaagcg
     1081 cggagcgcgt ccgcgcgggt cggtgggaca tgcattaact gaatcatgaa tagcacaacg
     1141 acataggccg cgccctgcac ccaaaaattg cccgccgcgc cgaagacggc gatcatcacc
     1201 ccgccgaagg ctggcccaag aaccttcatg agattaaatc cggcggagtt gagcgcaata
     1261 gcattggcga gttcggtttt cggaacgacg ctaggaataa gactttggcg cacgggttca
     1321 ctaaaggacc agcctatccc cgtgatcaag gtaaacacga aaatgtgcca gacctctaac
     1381 catctggatc cgaccagcgc agccatgagg aacgctgttc cgatcagcac gtattgggtg
     1441 cgcagcatca actggcgccg atccatgcgg tccgccgcca caccagccat tggcccagtg
     1501 attagaaacg gcagggcgcg cagcccattc aataatccca gcaataccga agagccggtt
     1561 agttcataca caagccagcc cagcgctacc tgttgcaccc attggccggc gctcatcatc
     1621 agagtcccgg tccaaagata gcggtaatcc aggtagccaa gggacgagaa agtttggaaa
     1681 cggaaacgct tagggatcgc ctgagttgga gcttctacgg ggtttgtgag ttgttcttcc
     1741 aaagatggtt ttcctgatcg aagttgtaat gcaggcgact tcatcattag caaacttcgg
     1801 tttagacagc cagcaaactt cgcggcagcg aactaagcca acgttgtgag aatcaagcgt
     1861 gttcatctaa atagtttttt gagaactccg aactacgttt ctgagaagcc aaagactccg
     1921 attaggaaac gcaaaacata acacgtttgt tcgattaagc cgaggaggaa ccaaacacca
     1981 attcccttcg ataccaagcc gcggcgcggc ggcccatgcc ccagttacat cagaacattt
     2041 ctcccagcct gctagctttg tgcgtcactc ctttgtgcta caaaactgtt ctttttctcc
     2101 catcaggacc ctagcagtgg cggttaaagg cctctcaacg tcagtaattc tatgacgatt
     2161 ttcgattgca caagttcggg gcacaagact tgctctctcg ggctcaaacg aggtaaccga
     2221 tgaaaaaact ttccaaaatt gcatgcctaa ccggggtcat ggcttgtctc agcatccaag
     2281 cagtcgtcgc gcaaacctct gaaaaagctt tcgatgccca gtgggttgaa tatagtgacc
     2341 atcatatttc cgttgcgttc gaccggattc ccgttgccga cgcggttcag gcgatccgag
     2401 agaagaccgg ctttcagttt agcctgccgt catcagcgga tagtaaattc ttgaaccttc
     2461 ggttggagcg ttcgccgcta gagcccgccg tgtattctct tctttctaac attggcttta
     2521 ggaattttgc cctgatttac gacgagcatg ggcgacctaa ccgtgcggtg gttttaggag
     2581 ctcaagttga cgaaccggcg cctatcctgg cggatgccag ccaggcggcc gagcctgtcg
     2641 ctcaacctct caacccggaa gagcgcgaca cactacaaaa agagttggat cgttggaccg
     2701 aactaaaaca ggaacaacga gggaggattg aagaccgctt gaaaaccttg ccgccttcgg
     2761 aagagcgcga gcaactggtt aaagaatacg gccgaaggct tttgggaata aaaaaataat
     2821 cgcctggcgg tcatcgtgac tgctgacgat cgcttgcttg agtcaaccat cgtgtcctcg
     2881 tgcctgttct ctgtttcctc tcaagccctg agaaatatgg tgccgcgcat ccaacacgta
     2941 gaagctaacg ggattgcgct ctcacacgta tccaccccac tgatctccgt catcgaaacg
     3001 atctcgaaga ctcgaggaga cgcaaacagc gctgctcatc tctctccggc tgcaaaagct
     3061 cagaacggct agatctagtc cacatcgggc cagcaaactc tcatctgtca gtttgttgta
     3121 aacgtcagct gatagttatc cgttagggcg cggcttctgt ctctccatga ggtattttac
     3181 gaggaacggc cagacttgag ttgcatccgc taaaacctcg gcatagatgc cgtcctttac
     3241 cgctttacgc catgattcat tttccgaata ggtgcagccg gacagatggc caaaatgagg
     3301 gcggtcagga cacactctga ctccgtaagt aaaccggcgc tcaggataga tcttgcccaa
     3361 gcgctcgttg atgatctcga tcagtggcgc gacgttttgc acgttgtttc gcggcacgcc
     3421 gccgccaatc gtgaaaatgc cgaaccgtct attctttgtc accagctcga ttagcaattt
     3481 gctgtcgagc tctaaatcca tcacgatcgg ttgctttcgg cggcgcttgc gccggatgtt
     3541 gtgaatataa agatcgttgc ccagttccga gtcgacgaaa gcgggcacga acaccggcac
     3601 accgtggagg taagccgatt tgagaatacc gcgatctttt ggataatgct cggcgaggta
     3661 tttgcctatc gctttattta gcagggtcgg gctgatggaa cgaacgccat taaactgctt
     3721 gatgacttcg ccgaccacct tctccaccgt gtccagattg gtttcgggct cgagcgtgtc
     3781 cgtcacccgg ttcagcttgt ggcgcgctag agcggtatca tcgtaagcgg gattgtattt
     3841 gtagtgcttc agtccgatgg acgaaacaag cccgtgagcc atcagcgcac cagttgatga
     3901 aaccgcttga acgaaaccca tttcgatcat atcgcaaatg agcagatcca ttttaccaac
     3961 cgtcatggcg ccgctcacgg tcagaaatcg aacgcattcg tcatcgctca ccatggcttg
     4021 cagcgctttt gccgcccggg agacttgggt cgcaacacca ccgtattcgt tgagctcggc
     4081 aatgagagcg gttacggaga ttgaccggcc gttaatccat tggtccaagg ccgagtaaat
     4141 caccgggatg acatagcgtg gatcagcaaa cacggcatcg ggaaagtaac catccttgat
     4201 ctgcccgggg cgagccatgt cgaacggatt gatgaaaata gcgcgcgccg ccttcgtata
     4261 tatggcatcg gcgtatcttt cggaaaacgc gccgacgacg atgacgtttt cgccgctggt
     4321 tttttgtttg gcatattgag agggcaggac aatcttttga gcccatttgt ttttcacaaa
     4381 ctcgcgcagg agtgagccga gtggggattc ttcggcgcca tcatagatga taatctgctt
     4441 ctcgttcgat ccgatcatct ggcggatcgt gttggccact tctcccagca tcctggcgcc
     4501 gaaagcgcaa tatctcatcc cggagacaat atcgccgacg gtcgcgcact cgcgcaaatc
     4561 caatgacttg agttcttcaa attcaggggt tttcaatcta tttctactgc gccaatttaa
     4621 tccgtgcttc gtaacacggc ggttgcatgc agtcaattgg aactgcgcct tcggcggctt
     4681 agccaaataa gctcaccgcg gcctcatctc aaatgagcct gatcgctgga ccgcagaacg
     4741 atgcggccca gcccttgaag gttttcgaaa atcagggttt gggtaggtat gcgctccggc
     4801 gcagaattta atttgtgccc ggccacataa gatcctcgaa aaatcggcgg tggtttcaaa
     4861 gacgaaactc gccgtagaac agatgttggt gtgcggcggc atatgcgttc tcaagaacaa
     4921 acgagttgac cgcgagaaac ggtggattgt atgctcatct gtgaggagga acatcgatta
     4981 tgaaattgtc cggcaaagtc gcctttatca cgggttttgg ttcagggctc ggacaggcta
     5041 tcgccgtgat gtttgccaaa gagggagcgg ccgtagctgg aacctcgacg accgaatcca
     5101 agggtcaaga aacagttgcg atgatcgaga agcgaggagg aaacgcgctg tttcgcgccg
     5161 gagatgtcgg tgattcagag cagatgcaat cgttgatcgc cgcggccgtc gagcagtacg
     5221 gcggcctcga tatcgttgtc aacagtgccg gagtcagaac caacggaagc attacggaaa
     5281 tatctgagca agcgtgggac cgcacgttgg acgtcaatct gaaaggagcg tttgtcgtct
     5341 cgcggctcgc aataccggaa atgaagaagc gcggtggtgg cgtcattctt catatcggcg
     5401 cccggtccgg catgctcggc caagcagggc gagccgccta ttgtgcttca aagggcggct
     5461 taatcaggtt gactgaggcg atggcgatgg atcacgccaa ggacaagatt agagtgaact
     5521 gcatctgccc gggtccggtg cggacgccga tggtggacac atcgacgcca gagaaattgg
     5581 cccgctataa gacgcgagtg ccgcttggac gcatcggcga gcctgacgac gtggcttatg
     5641 cggcgctgta tctggcatcg gatgaagcat cctatgtgac tggagcgata ttgccggtgg
     5701 acgcgggaat gcgcttgacc gggccgtgat tcgggagagt tctatgctga agcatctgcc
     5761 gtgttcagtc cttgctggcg aatttgcgtc ttaaccagat cgttcccaaa atgctgacga
     5821 ccaccaatgc agccaccagg acaaagctgc ccaatcctgg attacggatc gcgctctgta
     5881 actggtgcgc gaacaggttg gtgacgatta ttccgggcgc cagaccgagt agcgatccaa
     5941 agatgtaatc ccgaacgggt acttgaaacg cgccggagat tatattgatc accgtgaatg
     6001 gcgcgatcgg aatggcgcgc aacgctgcca ccgccaaaat tcccgtttgc gtgatcttcc
     6061 gttccagcgc cttccattta gctccgatca gttgccggat aaaatcgcta ccaagaaaat
     6121 agcccatggc gtaagtcact cctgcgccga tcatgcaccc gataaacgaa taaataatcc
     6181 cctccagcgg gccgaacacc aaggcagtag ctagaatcaa aatcgtgatt ggaagggaaa
     6241 tgatactgcc gatcagatag gcgccgagaa tgattgatac acgcgatggg ttggttcgca
     6301 gcgcgatcgc ccatgcggta attttgccga tgtcgacctg attggccaat ggcgtccagc
     6361 gccatgccgc cgccatcccg agaagcgcca cgagaactcc gccggcgatc catattcggc
     6421 gccgtgagcc ggtcgcggat cgttgaatgt gtgcggtcct cacagtctca tacttgcatg
     6481 aaagcttggg cacccatcag tggctctgac cagcataatg ggtttcccgt tactttcttt
     6541 ataacaaata attgccgcgt gattgagacg gaagcatcag accatcctcc gccagcatcc
     6601 aagcgaacgc agccgacttc tgtctcacct acccgacccg gatactctgc agctgtgccg
     6661 tttcgtggac aaacggattt gaatgaacta aacaaaagcc aatcacgtct caggggagta
     6721 cggataggag cacagcgctg ccttgcggac cggcggttct cgcgatgaga gcagcagatc
     6781 ggcagattcc gcacgccatg gaccggcgga tattagagag aaaattgccg ccggctctaa
     6841 agacagtcag ccagagtttc ccgcgcttat tccgccggaa aaccgaagga cggaaccgcg
     6901 ggcgcagttc gatgatatgg aattgatggt gtctccgcta gctggtttcg cctgcggggt
     6961 atttctgtaa acatgatcaa cggatcatcc tagatcacga gattcactaa taatcggaac
     7021 tcaacttacc catgactctg ctccttcggc ttctgctcgt cctgacgctt tcgtcgcact
     7081 cggcatggag cggcgaacta acagccgctg gcttgtggaa aaccgtcgac gatcacaccg
     7141 gcaagccgcg gggactaatc cgtatcgtcc aggtaagcgg cgaataccag ggtaaattag
     7201 agaaaatatt tccgcagccc ggagaagatc ctaatccaaa gtgcgacaag tgcagcggcg
     7261 cgcgccgcga tcagccggtg atcggcatga cgattttgtg gggtcttacc aagcagggcg
     7321 aggagtatca aggcggggaa attttggatc cagagaacgg caagatttac cgggccaaga
     7381 tgaagctgga agacggcggc aaaaccttgc acgtgcgcgg cttcatcggc atttctttac
     7441 taggtcgaac gcaagtgtgg atgagagagg aatgaggctt ggatgagcta cctactcatg
     7501 aaaattatag ttcgttcgag ctaaatcgct tggaggtgca tcatgcccga gaaaaaaact
     7561 ctcagaagag cgcgcaaagc caaacaggaa gggaaggctc cgtccaccca agcaggggag
     7621 ttcgtccggg aagagattga gcacgttcgc ggtggcaagc acggcgcccg ctcgacaaag
     7681 caggcgatcg cgattggtct atccaaggcg cggcgtgagg gagtaaagct ggcgccgccc
     7741 gctacaggga agacctcgcc aatgaccaga aagaaagctg cggccgatct taaagccgcc
     7801 cggaaaaaga aagtaaccac gaaaaagcgc tcgcgggcat ctttaaaggc gctgcgccgt
     7861 gaaggccggc gcgccgcttc gaaaaaatcg ttatcgcgcc aggccaagaa ggcccgctaa
     7921 acaaggtccg tcgagttcga gctatttcga tggtggagtc gtccgttaca ttcctgaggg
     7981 tctgtttgtt gtttgcggct taaaaccggg ccgggtctca taagtcccca gcggtcaagt
     8041 tgaagttatc cggacagatg aatgaagaca cttgtactct attcgattgc cgcaatcatg
     8101 tatgtcgttg gcggcgcgct gatgaagtat tcgcagggaa taacgcacgc tttgccgaca
     8161 ttgggtctcg ctgcgctgtt cagcgctggg gctttgatac aagcacgcgg catgcgctac
     8221 gaacagctca gctcaagcta catcgtggtg ttagggcttg aagcgctgct ggctatcata
     8281 gtgggaacgc tctggttcgg tgagcaatta tccgggcgcg ctgccgccgg gatcgctttt
     8341 gtagtgatcg gcattgtact tttacgcctg caatagctta cagagaagtc tggaacttca
     8401 gtgtgaattc aagatcgtac aacctcttgc aaccggctac gctgaagata ttctagtgga
     8461 caatggctga acgtaattac tatcaaattc tcggtgtgga acgcagcgcc tcgcatgatg
     8521 aaatccgcaa ggcttatcgc aagctcgccc gcaaacacca tccggatatc aatcccggga
     8581 acaaagaggc cgaaaacaaa ttcaaggaaa tctccaccgc ttacgatgtt ttaggcgacg
     8641 agaagaagcg caagttgtac gatgaattcg gtgaagcggg attggcggct ggtttcgatg
     8701 ccgacaaggc gcgtagctat caacaatggc agcaacagtc cgcggctggg gcgggtggtg
     8761 gttacgaatt cgatatggac gatttgagcg agatctttgg cggattggga ggcttgggca
     8821 gacgcggtcg gagcaacggc caagggccga tgcgtggaga agacatcgag tcgtctatgg
     8881 atatcgattt tctcgatgca gtccgcggtt ttcaaacctc gatcactctg caacggccga
     8941 tcacctgcga tacctgcaat ggctcgggaa ccaggcccgg cagcaagccg atccagtgcc
     9001 cggaatgcgg cggcagcggc agcaaatcgg tggcgcaggg accgctccag tttcgccaga
     9061 cttgtccgcg ctgcaggggc agcggaaaac tgccgggcga tccttgcgcg gtatgccgag
     9121 gttcgggccg agtgctgcgg gccgatacca ttcgggtcaa tattcctccc ggagccgagc
     9181 cttcgaaacg gattcgtttg cgcggcaagg gcgaggccgg agtgagaggc gggccgccgg
     9241 gcgacctcta cattatcccg cgaattcgac cccacccgct gctcacacga tcgggcaagg
     9301 atctatcgat ggacttgccg atcaccatcg gtgaagcggt tcggggagcg agcgtggaag
     9361 tgccgactcc catgggcaag ctcaacgtga aaatacccgc gggcgcccaa agcggtcaac
     9421 aactacgcct caagggcaag ggagtttccg cgcacgggca aagccctgct ggagacctgt
     9481 atttgcggct gatgatccgc gtgccccagg acaataagat ctctgcagac gtgatcgaca
     9541 gactcgatca agcctacgcg gaggacgtgc gcaaagacgt ccgactataa cgagagcgag
     9601 cgatggcaag aaaatatttt caaataacag aggtgatcaa aacctgcaag gtcgacaaaa
     9661 attttgtcca gcagctcgag cgtgaaagcg tcatttgccc ggtcgtgcaa cgaagacgca
     9721 aactctatcc gccggatcag gtggatcgca ttcggattgc caatcttctc atccaagaga
     9781 tgggggtgaa ccttgaaggc gccgaagtgg tcctgaacat gcgcgagcag atgatcgcca
     9841 tgcagcgcca gttcaacgac gtattgcgac tgatcggcca tgaaatgaaa cggtaggcaa
     9901 accgatcatg acatggctga ttgtcattct caaaaatcga gaagttaact catggatctt
     9961 gagctgatca agagtgacgt agtgaaggcc agccacatac tacatcgaga aggtgtcgcc
    10021 gcggctttcg gccacctgag cgcgcgtatt ccgggaaccg atactttcat ttttccgccg
    10081 cgaatgagcc cggctctcgt ccgggccgac aatctgctgg agctggacgt ggaaggcaat
    10141 caactctcgg gtgaaggccg tcccaacact gaattttgga tacatgcgcg gatttacaaa
    10201 gccaggccgg atgttcaggc gatttgccat gttcatccgc cgagctgcgt ggtcttgagc
    10261 tcactgggtg aaacgattcg gcccctgcac gcctcgggtg cgatctttcg aaacgatgtc
    10321 caggtattcg atcgaatcac tctaattagg acgcgcgagc tgggcgatgc ggtggcgagc
    10381 acattaggcg atcacggcgc catgttactg cgcggccacg gtgtcaatgt agcggataag
    10441 gacgtccggc gcgtgtgtgt catgaccttg tggatggagg aagcggcaaa ctaccaactg
    10501 cgagcgatgt ccgcaggcaa accgcgttac ttcaccgccg aggagctcga aaccatttat
    10561 ccccaggtct ccggagaaga ggtgtcaaag cgcgtttggg agtacctcag cagccgggtc
    10621 aattagtagt tcatatcgtt tgcattgtcg acgcgttcca cgggttcgcc tatattacgg
    10681 gtccgaattt ttacctgcta ctaatatcgc gatttaccca gacggtatgg cggcggtgaa
    10741 atccatcgac cttaagaact gcatagatcg gcgtactcgc cgagcgctcg accgaagccc
    10801 gcggcatcca agccgtaggg gccgaagtcg ccggtgaatg cagccagcgc ccagacggcg
    10861 gcgtctcgat atcgactctc tcgtgtcgct tcagcgagcc tcaggaagaa agaagcggct
    10921 gcgccatttt gcgcgatcaa cgtgatgcgg aaagcggcga gagcaggacc cgggatttcg
    10981 atatcgtagt atccgccctg cgggtttttc agctgcgcta gaataaattg accgagcgca
    11041 atcgcgcgct cgagatactt ggtgtcttcg gcagtcgaat acgcttttag cagcgcgttt
    11101 cccatgtgga tttggtcgtc gagtaatccc gcgacctggg ccgcgccatc gaagtagtga
    11161 gccatggcgc cgttgcggct ccggcaacgt tcccacaaaa actctagcgc gtctagcgcg
    11221 cggtcacggc agcgcggttc cgggagaatt acctcggcgt cgagataggc gataaccgtt
    11281 tgggcattgg catcgatgta tatagaatca tccagaatcg aaaaaaattc ttcatcggaa
    11341 gaccgctcgc cgtcctcttt tcgcagaaaa tcctcgcagc cgaaaaagcc gccggtggcg
    11401 gtgtcgaata ggtgtgcgtt caagtaggct atcagttctt cggccatgcg cgcataaacc
    11461 ggtagctgcg tgttttgaaa cgcccgcagg cagttggcca gaacgccggc ttgctccgcg
    11521 agcagtttct cgcgatgcgg ccggctccag tcgcggccgg agcaggtgcg gaaatagccg
    11581 cccgcttcag ggtcgcgcat cgggctctcg cgcatgcgat ccaaggtcag gcacacttgg
    11641 tcgaggtacc gagattcttt ggttgcctcg taacgagcga gcaaaaagtc gttgacctgc
    11701 ggctggatga atttctggcc gcgaccataa ccgccgtatt cccggtcggc aagatcaagt
    11761 atttgtctag cgatgttcgc aattgcgcca agatccggct gtggatcatt catcgattgt
    11821 cgtatgttgc cgctctcttg cgcgtttcga tggagcaaac gaatttgatc tttcttctcg
    11881 tgataagccg tgtaaatgcg aaccaggaga tcgccgaact gctcgctcgg aaggtagtta
    11941 gttacggaga gtaggtcccc atcagcagtc atgaaagcaa tggtcggcca gccgttacga
    12001 ttgtagcgaa tgtcaatgtc gggtcgttgg gcattgtcac agcggaccga aatgaaataa
    12061 gcgttgagga gcgccaaatt ttcgttgtcc gagaatgcct cctcgtccag gcgctggcag
    12121 aagcggcacc agaatgccgt aagaagaagc atcaccggct tgtcctggtt gcgagcgaga
    12181 tcgaaggggt cctcacccca ttcgtgccaa tgcactagat gcgccctatt tggaatgggt
    12241 gaaaagcgga tcatgcgcgc agttttcaat caagggataa cgttcttatc accactggcc
    12301 tcacaaaaag caatgacata atttgaagaa gagttcgatg gcgttgacat tcgtattgac
    12361 cgagccataa gatcgactcg acctatttgc gcagctattc accgaaggac aactgaatgc
    12421 aactcaccct cgcataccat ccggtcagcg aaatccgttt cggctcccgg acacggctcg
    12481 atgacacggt gctggcggtc gacgccggcg aactgcgcag gcttgtgctt gaggatgagg
    12541 cgcttgccgc cgtggatttc gacatagtcc ggccgggcga cagttgccgt gcaggaccga
    12601 tctacgacat cgtcgaaccg cgcgcgaaag caccggagtc gggtgccgat tttcccggga
    12661 tcctcagcgc tccaacgacg gcgggtttgg ggacgaccca cgtgcttgcc ggttccgcgg
    12721 tatcggtctt ggccgagatg tcaccgggag aggggagaag cgccaccgga cgtgttttgg
    12781 agatgagcgg tccggtggtc tcggcaagcg attattcctc tttacagcat atcttgatca
    12841 ttccgcatac caagcctgag ttgccccgcc aggtggtatt gaaggcgtat cgcctggccg
    12901 cgcttaaagt tgctgtacag atagcccgtt gcgcaatcga tcgagctcca gcatcggtac
    12961 gaagctttcc atctgtgggt ccgtcgttgg gagatcgcga cgacctctca cgcgtggctt
    13021 acgccggaca aattttttcg cgtcagcgta agcccggtgt cgacgagccc attctctatg
    13081 gcgccaacac ggaggggatg ctgccggtcg tgctgcatcc cgacgaatgg ctcgatggcg
    13141 ctgtcgtgcc atcgctcaac tcctggttcg gcggcaccga gacttatttt tatcaaaatc
    13201 atccggtcgt tctggagctt taccgccgtc atgaggcgcg tgaaataaat ttcgtcggaa
    13261 cgattgccac gatcgccggc tcggaccagt tcgatcggga gcgacactgc caagcagcgg
    13321 cgcatctagt gaaatggaac ttacgcgcgg acggcgcggt gctgaccaaa tacggcggcg
    13381 gcttgccgca tgccgatctg gccgaaacag cgcgattgct cgaaggaatg gggattcgaa
    13441 cggcggtgat ggtttccgat tcgtcgcgcg atcgccgggt cgagtcggcg ctgctgttca
    13501 attttcccga agtcaacgcg attgtttata acggcggcaa cggcacccaa tgggaagtcc
    13561 cgcgcgtcga acgggtgatt gcggcaactc cggagttggc agaactgctg gccggaccga
    13621 tggcgatgga ggcggcgaat attatcggcg taaccaacca gcagggggcc tcccgcatga
    13681 ggtcgctggt ctactagaat cattccgggc tcggatgggg tttgaaagtg aggatgcaat
    13741 gagagtcgtg cattatttga atcagttttt tggcggcatc ggcgccgagg agcatgccgg
    13801 cgcaccgctc caagctcgcg acggtgcggt ggggcccggc aggttgctgg aggcgctgct
    13861 gggtgatcaa acccgcatcg tgatgaccct ggtttgtggc gataattatg ccgtcgaaaa
    13921 tcagcaggca gttaccgcct ccgtggtaga gaagatgcgt ccgctgagag ccgatctgtt
    13981 cgtcgccgga ccctgcttcg atgccggccg ctacggcatg gccgcgggcg cgctgtgcgc
    14041 agcggtgcaa gcggagttaa acattcccgc tatcaccggg atgaatgaag agaatcccgg
    14101 cgccgatctg tatcgcgatg cgctctacat catcgactcc ggcagcaatc ccgccaacat
    14161 gcgcgaggtc ttaggaaaaa tggccggcct gggacgaaaa ctggtgcaaa aacgcgccgt
    14221 gggtttgccc gccgaggaag gttaccttcg ccgcgggctt ctgcgcgacc aggtggtgaa
    14281 acacaccgct gcccgccggc tggcagacat gctggtagcg aaacttaacg gcacgccctt
    14341 tgagtccgag atgctgcctg tggcattcca accgacgcca atgcctgcgc cgatcaagaa
    14401 cctttcacaa gcgcgggtga tgctcattac cgatggcggg ctcgtgccca aaggcaatcc
    14461 ggacaaaatc cagggcacgg ccgcgacccg ctggggtgct tacagcttag aaggacgcga
    14521 tgacctgcgc ggccaagatt acgaaatttc ccacggcggt tatgacgccc gctttgtcca
    14581 agaggatccc gatcgcctgg tgccgctgga tgccatgcgt gaaatggaaa agcgcggtgt
    14641 tatcggcaag ctgcacaacg aatttatttc gacttcgggc cgcgtcaatc cgctttcgaa
    14701 tacccgccgt ctgggtcgcg aaatggcgca gaagatcaag agggacggcg ttgacgccgt
    14761 gattctgact tcgacgtgag ggacgagcac gcgcagcggc gctgcgatta caacggagct
    14821 ggaaaaagcc gggattcccg tcgtgcaaat tacctcggcc ctgccgattg ccaagatgat
    14881 cggatcgaac cgcatagtgt tgggaaatgg cattgtgcac gtcaccggcg acgccaaact
    14941 gtcgccggag gatgaaaaag atttgcgccg caggctcgtg caaaaagcgc tcgacgcctt
    15001 acaatcgagc gaaacgcaaa cataattatc cacgctctcc ggcatcgtca tagaattggg
    15061 catcgcacgc atttgacgcg cttcgccgtc ttatgacacg atcgggcgcg aattctcggc
    15121 atctctgaaa ccggagcgct gaggaacttt taatccaaaa gaaaggtagc cacatggaaa
    15181 aaacagcgaa tgagaaacgt aagacgctgc gcgcattgct gcaggggccg ggatgctcga
    15241 ttgcgccgag ctgtaacgat ggcatacaag cgcggctggt tgcgtggttg ggttttccac
    15301 tggtccatat ttcgggcagc ggccaacatc gcgctctcgg ttttgccgat gccgggatgc
    15361 tcacgctcac cgagatgatc aatcgcgcgc gcgagatcgt cgatgccgtc gacctgccga
    15421 tcgtcagcga cggtgaaacc ggttatggca acgcggtaaa cgttgtccga gctatgcggg
    15481 aattcgagcg agccggcgtt gctgccatac acatcgaaga ccagatgacg ccgaaacgcg
    15541 caggtcacga aggttttgat gttggcctgg tttccaaagg cgagttcgtg gccaagatta
    15601 aggcggcgct ggatacacgt cgagatgaaa atctcgtcat tatcgcgcgc tccgaggcca
    15661 aggattcatt acaggagcgg ctcgaccggg tcggcgcttg ttgcgaagcg ggagcggacg
    15721 cagtctggtt gagcgcgcgc actgaggacg agatcaaggc gttcgctaaa cttggcaagc
    15781 cgctcgtcgg ggttccgcct cggcagctaa tgaccatcga tcgctacggc gagttgggcg
    15841 gccgggtcgg ctgtatcccg acgattctcc aggtcgccgc gctgcacggc atgcgccagt
    15901 gtctcgaaga gttgaagcga agcggcacgg aagcacgcta tttcaaggaa accccgggta
    15961 tcgacgacac ccgcaagtgg tacaccaatc tcggtaacca ggaacttaag gagctcgaag
    16021 cgaagtattc ttattgagga gctgagaccg actctggcta caatcatcat cgcacccgaa
    16081 gcggtccgtg gatagcgtta caatagttat cgccgaatac gcctccatct cggtgttcga
    16141 gcatctcacc ctggaagcgg tccatgccgc caagcagcgg ctgatcgatt cggtgggttg
    16201 cgcggtaggc gcgcacgatt gccaaccggc gcaaatcggc aggcgattgg cgcgcggtca
    16261 aacgccggga aaacatgccg gcagagtgct ctattatgga gaccggctac cggtcgacag
    16321 tgcggcgttc atcaatacgg cgatgattcg ccatttcgat ttcaacgatc gctatccggg
    16381 tggtcacccg agcgattgct tgggcgccct gttggcgatg gccggcgcca tggatatcga
    16441 cggcaagcgt tttctcgcca gcatgatcgt ggcctacgaa atttttgctc gattgagcga
    16501 tgccacgatg ctcagccggc gcggctggga tcaaggctac gccatcggca tcgcgactgc
    16561 ggcgggagtc tgcaacttgc taaaaatctc gcccgcggca accgcgcatg ccgtggaaat
    16621 cgccgccgct tccaacttgc cattacgggt gacgcgatca ggcgagctca ccccctggaa
    16681 gaacgcggcc acggcttacg ccacgcgcaa cggcgttttt gccgcattgc tcgccgccga
    16741 aggaatgagc gggccggcca acgcgttcga aggacgcaat ggcctctttg aaaaggtaac
    16801 cggaccgttc gagctcgcgc catttccaaa ttgcggcggc gcctatctta cgccgagagt
    16861 tcaattgaag tactggccca tcgaaaccaa cggccagccc gcggtttggg cggcgctgga
    16921 gctccgtgaa aaagctcaga tccacgagct caaagagatc gaggttttta cctccaagtt
    16981 cacctggttt gagatcggca gtgagcccga gaaatgggac ccgcagacac gcgagaccgc
    17041 cgatcacagc ctgccttaca tattcgctag aaccctggcc gatggaccga tcaagatcac
    17101 atcgtttgca gatgatgctg tgcgtgatcc cggattgcga tcccttatgt cgaagatcaa
    17161 ggtgatagcg gacgatcgca tcgaggccct gttgccgcag aagaccttta tgcgggtggt
    17221 cgcgacgacc ctggacggac gccagcatgt tgtcgagatt gccaatccac tgggccatcc
    17281 ggacaacccg atgcaagaca gccacgtcga agaaaaattt attgctttgg tcgagccggc
    17341 gctgggcaag acacgttgcc gcgcggctct cgactcttgg tggcgcctcg acgaagcgac
    17401 tgaagttagc cctgcaatgc agttgctaga caataatcgc tagcgcagtc aaccccgcct
    17461 tcgaaataaa gcagtgggac ggcgccgcaa tgccgggtgt tttaggttct tacgaacgtg
    17521 gacttgttga agcgagaaat acatctgagt gtcaggcttt gggccagagg ttattcatga
    17581 gcgcggatca aaacactttt gctccagctg ccgaaggact gaagacgaca gctgcggcag
    17641 ctactttcat tgagcaggtt acggccggtg atctgccgag cgaagcggtg cgcatcggca
    17701 cccgctgcct gctcgacggg ctgggtcttt acgtggctgg ttcgcaagag cattcggttg
    17761 agcttctcat cgaagaagcc gagcacgtcg gcggtcgcgc ggaggcgatg ctgttgggcc
    17821 gtggcaagac caaggttccg gcgtcgacgg cggcccgtgt tctcggaacc gccggccatg
    17881 cgcacgactg ggacgatagt caggtgagct ctgatcctac gcatgtgtac gggctactga
    17941 ctcatccgac gattccgccg ctgaccagcg cggtagtcat gagtcagcgg tcgggcaaca
    18001 tcgacggtca ggcctttatg ctcgcgtttc ttgccggatt cgaggtcgaa tgtaagattt
    18061 cggagtggat gttgccgcag cattatctga gaggattcca ctctagcggc accgtcggaa
    18121 ccttcggcgc ctatgccacc gcggcaaagc tcctgggctt gaaaggtgga agtctgcgca
    18181 gcggcttcgg catcgccgcc agtttcgccg cgggcattcg ttgtaacttt ggcaccatga
    18241 ccaaaccgct gcacgtcggc agggcggcgg aaaacggcgt caccgcggca ctgctcgccg
    18301 cgcgcggttt cacagccgat ccggatgctc tggatggggc ctggggcttt tacgcggtgc
    18361 acggaggcgg cgttagcgct gaaaaaattt cacaggactt cggcaaaatt tggacgatcg
    18421 ttgagcccgg agtgtcagtc aagccttatc cctgcggtgt gctgactcat ccgactatcg
    18481 atttgatgtt gaagctggtg accgagcacg agatcaagcc ggatggcatc gaagcggtta
    18541 cggtttatgc gggaacaaat atcctaaagc ccattcgata tccgatcgcc gcaaatcacc
    18601 ttgaagcgaa gttctcgctg ccggctgctc tggccatgat cgccttggct cgcaaagccg
    18661 gcaaacggga attttccgac gagttcgtgg gctctccggc catggaggca atgcagcggc
    18721 gcatatcgac cgagtttgat cctgaaatcg aaaagcgagg atttgataaa atgcgctccc
    18781 gcatcaggat cgatctcaaa gatggaggta agatcgaagg gtgggccgat gagcgctatc
    18841 gcggcggacc ggaaaaccca ttgagcgatg ccgatctgga agtgaaagtg cgctcctgct
    18901 gtgaaggtgt tctggatgaa aagagtcaga cgactttgat cgatgcggcg tggtcgatcg
    18961 gcaagataaa ggatgccgcg cgcttgatgg agattatcaa caagctgcct tgaacgatag
    19021 gtcggtgcag ggctggtggt ccaaagacgg attaaggaga aactatgatc gacccgattg
    19081 aagcaatcaa agaaaagatg attatggtct gcaagattct acaaaagcaa agagtcatcg
    19141 acgggtacgg tcatgtcacg gcacgtctgc cggatgggcg tatcttgagc acgccgcata
    19201 tgccgccggg aaaagtcgcc gtgcgcgatc tgatcatcat cgatggtcaa ggaaataaac
    19261 tcgaaggcgc cggcgaaccc aacggtgaga cctcgatgca tacttcgatc tataaggctc
    19321 ggccggatgt ccagtccatt cttcattatc atgccgatga actcattgcg gtggcggcca
    19381 gcggccaggg gatcaaggtc ttgtccaatg ccggcgtgca cttttatcgc ggcacgccga
    19441 tctatgattc gccgctgctg atccgcaatc atcaactggg cgacaaagtg gcggcgaccc
    19501 tgagcgaccg cagcgccgtg ttgttacgcg gccatggcgg cacggtggtg gcaaaaaatc
    19561 tcgatcagct ggtgcgccgc gggctcgacc tggttcgctc ggcgaagctc cagatcatgg
    19621 cgtctactct cggcccgctc aaaacccaca gcgatgacga atgcgagcag atgatccagg
    19681 ggcatgacgg acgcgatgcc cagcggcgct ttctcgacta ctatatttcc gaagttgtcg
    19741 attgagcggc cccaagaccg ccagctgcaa tcagcaagtt ggaagatagc ttccggcgcc
    19801 tgcatcggat tctaacccgc ggatattccc aaaggccgaa atggacttgc aggttcctcg
    19861 attcccagat aacaaagaat tttccggtca ctggcgaaaa ggcagatctg caaggcacag
    19921 ccacggtgag atgaaactgc agcttcttag aagacttttc agattgagca gaaggagaat
    19981 catgcatgag tgaagcatcg aaaacagaca agagcgctat caaaaaatct acccagcgct
    20041 ggcaatccga cgtcatcgtc gatctgatca aacagtacga tttcccctat atcgcgctca
    20101 atccgggcgc gagttaccgc ggcttgcatg attcgttggt aaattatggc gagaacgatc
    20161 cgccgatgct gctctgcaat catgaaaaaa tcgccgtgca gatcgcccac ggttatgcca
    20221 aagcaaccgg caaaccgatg atggccatcg tgcacaatgt cgtcggtctt ttgcatgcgc
    20281 cgatgggaat ttactatgcc tatatcgacc ggtgtccggt cttcatcgtc ggcgccaccg
    20341 ggccaatgga tgagaaactc cgccgcccgt ttatcgactg gattcacaca gcgttcgccc
    20401 agggagacgt cgtccgcgga tttaccaaat gggattacca gcccggcagc attcatgggg
    20461 tgccggattc gtttgcgcgc gcctattcga tcatgatgag cgagcctcag gggccggtct
    20521 acatggttta cgattcggcg atgcaggaaa gcccgatgaa agaggagatg gcgctgccga
    20581 aattcaaagt aaaggtgcca tcgcgagtcg ctcccgagcc ggcggtcatc gaagaagccg
    20641 cggacatgct catggcggcg gagaatccct tactgctcac ggagtacgcc gcgcgcatgc
    20701 ccattgggtt tgattcgacg gtggaactgg cggaaaccct gggcgcttcg gtgttcgata
    20761 tcaacaagcg cctcggcttt cccaaccagc atccactgag cgtgagcttt cacggcgatg
    20821 ccttcaaagg cgtcgatgtc gtcgccgcgc tcgatgtcat cgactggacg cgcggctcgc
    20881 acaagcttaa cactcagaca cgtgaaatca gcgtagtcac cgcaccggat tgcaaatgga
    20941 tcgacgttgg cttcgccgat attgagatca gcaagtgggc gaccgattac aacaagcacc
    21001 acaactggga tctacgagtg ctcggcgaca gcgccctctc gctgccggca ttgacaaccg
    21061 cttgccggaa gcggattgac ggcaataaat ccatgactgc aaagatcgac gagcgcaaaa
    21121 aagccgttgg cgagaggcac gcgcgccaat ggcaaaaatg gcaggaacag gtaaagcagg
    21181 agtggaacga gcggcccatg accgaatcgc gcctggcgca tgaggtctgg caagtaatcc
    21241 agaatgagga ctgggtgctg accgccaata ccctcaagga atgggtccac aaaatatgga
    21301 acttcgaccg ctcgtatcgc cacgtcggcc gcgagcttgg cactggcact caaatcggca
    21361 tgtccatcgg tgttgctctg gcgcacaagg gctcgggcaa actcgtcatc gacctgcaac
    21421 ctgacggcga tttgatgttc gatcttggcg cgctttggat gccgatcaag taccagattc
    21481 caatgctgat cgtgatgtat aacaaccgcg cgtattacaa cgattgggcg catcagattc
    21541 acatggcgca tcaacgcggc accgaccccg cgcgcgccta catcggcatg gatctcgaag
    21601 gcccgccgcc cgatttcgcg catatagcca aaggcattgg ctggcacgcc gagggtccca
    21661 tcgaagatcc ggccgaagtc ggcccggcgg taaagcgcgc aatcgcacag gtgaaggccg
    21721 gcaagccggc cttggtggac acgattgtct ggcgtcgtgg cgagaacgcg taggaaaaga
    21781 caatctaacc acgaaggtca cgaagagcac caagggaaag aattctttga cgagagaatt
    21841 tcttcgtgac cttcgtggtg aaaaaactta gttgatcgca aggaggaaac ttccatggca
    21901 aagcctgctc cgaaagcaga gaccatcaaa aagtccgaca agcgttggca gtccgacgtt
    21961 atcgtcgaca tgatcaagcg ctacgatttc gagtacatcg cactgaatcc cggcgccagc
    22021 taccgcgggc tgcatgactc gctggtcaac tacggcgaga atgacccgcc gatgctgctt
    22081 tgccagcacg aaaaaatcgc cgtgcaaatc gcccatggct atgcccgcgt caagggtaag
    22141 ccgatgattg ccatcgccca taatctagtt ggattactgc acacgccgat gggtgtctac
    22201 tacgcctacc tggatcgggc tccggtgttt ctcatagggg ccaccgggcc gttggcggag
    22261 cccaagcgcc gcccgtttat cgattggatc cacaccgcca atgttcaagg ggaagctatt
    22321 cgcaattttg tcaaatggga ctaccagccc agcaccgtcg atggtgtgcc gggcgcgttc
    22381 gcgcgcgcgt attcagtcat gatgagcgcg cgacagggtc cggtttatat gtgttacgac
    22441 gccgggctcc aggaagcgca actggatcat gacgtccaga tgccgcctcc cggagcaatc
    22501 gacgtcccga ccgcgccctc ggccgatccc gccgcgctcg agaaagccgc cgatactttg
    22561 gctgctgcca atcgagtggc gattatcgcc gatttcgctg cccgcccgcc acacggctgg
    22621 aatcatgttg tcgaactagt ggaaactttg ggcgcctccg tctgggatgt gggctcgagg
    22681 ctcaatttcc cgaccaatca tccgctcaat ctcaccatgg atcctgaagg ttgctataag
    22741 gatgtcgatg tcgtgctaac cctcgatatc gccgacttcg agaagccgac tcatacacgt
    22801 gacatctcga ctcgtaccgt cgtcagcaag gttcaggaga aggcgacctg gattgatatc
    22861 ggctttactg acatcgaaat cagtaaatgg tcgatggatt acgcgcgtcc gttctatgcg
    22921 caacagcgca tgactgccga cccggttacc acggtgccgc ttctgaccaa gcttctcaag
    22981 gcgcgtatcg ccaattccaa agggcttaat gaaaagattg ccaggcgcaa agaagcgatc
    23041 ggcaaacgtc atgctgagaa tcgcgccaag tggagcaagc aggcgcaaga gcattgggat
    23101 gccaagccga tgactgtgcc gcgcttggcg ctcgaggtat gggatgcgat caaaggcgag
    23161 gactgggttt tgactaccgg agatttgggc agctggggaa agaagctttg ggattttgac
    23221 cggtcgtatc gtcatggcgg ccgtgagctc ggtaccggca cgcaaatcgg cctgtcactt
    23281 ggggtagcgc tggccaacaa gggaaccggg cgcctggttg tcgatttgca gccggacggc
    23341 gatctgatgt tcgatgccgg agctctgtgg atcgcggcaa aatacaagat tcccatgctc
    23401 atcgtcatgt tcaacaatcg cgcctattac aacgattgga accaccaaat tgtcatggca
    23461 aaaaatcgcg gcaccgatcc gtcgcgggcg catatcggta tggatcttta cgaccctgat
    23521 cccgactttg ctggtctcgc tcggtcaatg ggctggtacg ccgaaggacc gtttgaaacc
    23581 ggcgatgatc tgaagccggc gctgaaacgc gcgatcgagc aagtcaaaca gggcaagccc
    23641 gcgctgatcg atgcggtctg cgaccggcgc aaccatggct aatagctaat cagatgaacc
    23701 aggcgcattc tgccggctca ggcgatggcg gctttataaa gttaacgttt tacattagaa
    23761 aaatattgac aaaaatatcc gtgatcaggt aactatctgg ctagataaac gtgtcgtttt
    23821 gcctggtgct cgttcattct aataatcaaa aacggtgtct cgtacggggg taagatatga
    23881 aaggtttttt ggtcggattc gcatggctga tgggtgtaac gctaacgggg ttcgctgccc
    23941 ttggggccga cagttcgccc gcgtacaatt ggaccggatt ttatctcggc gtcaatggcg
    24001 gcggcggttg gggtcactcg cgtcatgatt tcgaggcggc gggcaccaca accggagact
    24061 acaggatccg gggcgcaacc gtaggcggta cgctcggagc caatctacag gcaggcgggc
    24121 ttctcctcgg ccttgaagga gatatgaatt ggtccgacat tggcggcagt gattcctgcc
    24181 ccaacgtgaa ttttacctgt cgaacccgaa acaagtggct tgccacggct cggggccgtg
    24241 tcggatatgc acttgatcga ttcctgccct atatcactac gggcgcggcc ttcggcgatg
    24301 tccacaccga aattcctgat tttggaagct cgagggaagc cgaagtgggc tggactgtag
    24361 gcggcggact ggaggcgggg atagttcgcg gactcagtgc aaaaatggaa tatctctatg
    24421 tcgatctcgg caaattcaat tgcggctttt catgcactgc caccggagtc gatgacgtta
    24481 agttcaacgc tcatatcgtc cgtatcgggc taaattatcg gttctgaccc aagtcataaa
    24541 tgggttctcg gaacgccggt gatttaccgc ttgctaatac tcttcatata atggcgttgc
    24601 cgccttctag agcattggga gactacttcc cattaagccc agtcgggtgg cttatccggg
    24661 ctaagccgca acggagttcc ggggcggcct ggcacagaaa aataaacgca aggttctgct
    24721 actcgcgctg gatctgaaat tcggccctac cattgataag catgccctca agcggtgaca
    24781 agctaaattg ctgggatgct ggcagagggt ttactggatc aatcctcatc aagctgtcat
    24841 cgactcgagc gggaaacaaa gtacttgaga cctaatctcc gatcatgctg aaattgtcgc
    24901 tcacggccat ggccattgcc ggcgctctgt ttaaagccac cgggtttctg ttcgtctcac
    24961 taatgaatct cgtctttcgt ccttacggcg gagcctacct cgctttgtta atttcaatct
    25021 atcccggcta cgatccagtc agcgtgcctt taggacttgt cgtcggaact ttctattcac
    25081 tgcttagcgg agcgcttgcc ggccttctcc ttggttggct ctacaatttc tttgcgccaa
    25141 agatttagta tcgttgctgc gtaatcctgt agaagtctgt tgcagcgttc tgccggagtt
    25201 ccgccaagga tttttctccc tcccggcgct ccaatgccgt ggcgtggccc ccgcctcaac
    25261 agcggcggtt taagtgatct caggcctggg cagacccaaa gggaggtcca tccgtcagtt
    25321 cgagcgttca ccgaatgcag tcattgaaac tgttgcaaaa agctcgctga tgccagagcg
    25381 gtcaatcgat tcaaagctgt caacggacat gagcactcaa tgacttggaa gcggaagctg
    25441 aatccatcga agcggaagcg atgtgggcgc tatctcgatc atgcttggca tttgcgctcg
    25501 cgggggtatg gttattgagg attgagcatg cacatcttgg ttgtcgaaga cgaaacaaag
    25561 gttgcgagtt ttatcaggcg cggactcgag gcgtccagtt attcagtcga tgtggagcac
    25621 gacggcgctg ctgggctaaa gcgtcttctc gagcatgact acgacctgat cattcttgac
    25681 gtcatgctgc cgagactcga cggtctcagc ctgatgaagg aaattcgtca aaggcagatc
    25741 aacattccgg tgcttttgtt aacggcacgg gtcaccatag ccgacaaagt gatggggctg
    25801 gatctcggcg ccgacgacta tctcaccaaa ccgtttatct tcgaggaact gctggcgcgc
    25861 gtgcgtgcgc tcttgcgccg cagcactagc gcgccagcgc tgcttgctgc cgccgacctc
    25921 cgactcgatc ccgccacgcg cgaggtgacc cgcggcggca agcggatcga tttgactccc
    25981 aaagagtttg cgttgctcga gttcctgttg cgccggcacg atcaggttct aagccgggcg
    26041 gtcatcgccc agcatgtttg gggcgtcaac tatgatactt tcactaacgt gatcgatgtc
    26101 tacgtcaatt acctgcgcaa taaaatcgac aacggttttc aaattaagtt gatccatagc
    26161 gtccgcggtg tcggctacgt gctgaaggca gattcgtccg tatgattggg catcctagca
    26221 cgttacgcgg tcgtctgacc ctttggtaca cggggattct cactgcgacg cttgccttgc
    26281 tgggcgcgat ttcggttgtc ctgctcgacc gcggcctgag aaataacatc gatgactcgc
    26341 tcaactcagt tgctcggaca atcgccgaat cggtgcggcg tccggcatct ttcggcgata
    26401 tcgaagattc gctgcaaggg ctgcttggtc ccgagttggc ggaacgtttt ttccaactgt
    26461 tggatccttt tgggcggctc gatccgcggg tagcgccgcc gggaagatca cgtttgcctc
    26521 tcagcccaac ggctcatcaa aatgccgagc gaggacagcc aacttgggaa accgtggtga
    26581 tccgccccgg atccaacaag ccattcagac tgctgacttt gccggtggtc aggaatggcg
    26641 cgacggtgaa tatcgttcaa gtggcgatgt ccttggaaaa tgtcgatgcc gcccgttcca
    26701 ggttcctcct cattctgctc atcctcgctc cagtggctct ggccgcatcc gcactcggcg
    26761 gctggtttct ggcgcgccgc gcgctcaaac ccgtggatgt gatggtggac gcagcccgcc
    26821 ggatcgaagc ggaagatttg tccaatcgac tgccggcgct gcccagtgat gatgaattgg
    26881 gccggctggc aacggtactc aatgatatgc tggcgcgact cgagcagtcg tttggcgctg
    26941 tgcggcggtt tagcgctgat gcggcgcatg aactgcgcac gccgctgacg attatcaaag
    27001 gtgaaatcga ggtcgctctg cgatcatctc aagatccagc ggaaattcgc caggcgctga
    27061 gaagttgcct cgaggaggtg gaccgattaa acgcgctcat ggaggacttg ctcttgatgg
    27121 ctcgcatgga tgcaaaggcg ttaagcgcgc cggcgagcct ggtcaatctc actgacgtgg
    27181 tgcaggaggc cgctccggca ttgaccgagc tggcgattcg tgcgggaaac cattgcacgg
    27241 ttgttgcgac tgcaccgcta tgggtacacg gttacgagtc cctgttgttc cggctcgttt
    27301 tcaatctggc cgagaacgcg atcaagtata cgccccgccg cgggaccatt gatgtcaccc
    27361 ttgagcagca gggtagcgcg gccgttttgc aggtcaaaga caatggcgca ggcatcgctg
    27421 cagaagcgca gcagcacatt ttcgatcgtt tctaccgcgg agatccgtcc agagaaggca
    27481 atggtaccgg acttggattg gccctggtcc gctccatcgc cgaactccac catggtcaaa
    27541 tcagcgtctc cagcacgctt ggccagggca gctgttttcg cgtggttctg ccgcgtacgg
    27601 ccgcaccttt atcttcggcg ccacaagatt agaaaattct aatcctttcc tcattaacct
    27661 ctaatcgttg cgacttaaac tacgctcgta aaggtactag cgttttatcg ttgctaaaac
    27721 gaaggaggag aagaaatggt atccaagaaa aaaattattg cgactgcgtg ggccgccgcg
    27781 ctggcgggcg gtgggtttgc aattggcgga gcaatccaga acagcagcgt gccggtccaa
    27841 gcgggcgcaa ctgaacccgg gaccgcgtcc cgtgcttctt tgccggcgtt tgccgatctt
    27901 gctgagcgcg tctctccggc ggtggtcaat atcaaggtga ccgcggttgc gagggccggt
    27961 gtgcaggacg actcttttgg cgaagagttt ccgtttcctg ggtttcgcag tcctattccc
    28021 caacagcccc gtgagttcag gcgagaaggg agtgggtcgg ggttcatcat ccgcaaggat
    28081 gggctgattc tgaccaataa tcatgtcgta gaaaaagctc tggagattac ggtgactttg
    28141 agcggcaaac agcaatacaa agccaagatc ctgggacgag atccgaagac cgacctggcc
    28201 gtgatcaaga tcgatccaaa agcggcgctg atcaccgcgt ctctcgggaa ctctgatgag
    28261 cttagagtcg gcgactgggt gatggcaatc ggcaatccgt tcggcttgag caacacggta
    28321 acctcgggaa tcgtcagcgc caagggaaga acgatcggcg ccggaccgta cgatgatttc
    28381 attcagaccg atgcatcgat caatccgggc aactccggcg ggcctctttt caacatggct
    28441 ggcgaggtgg ttggcatcaa cacggcaatt ttcagccagg gcggcggcaa tattggaatc
    28501 ggctttgcca tcccggtaaa tctggtgaag aatctggttc cggaactgga gaccaagggc
    28561 accgtcacgc gtggctggct cggggttacg gtacaaccga tgacgccgga actggctcgc
    28621 tccttcggtt tagataaaga gtacggcgcg ctcgtgggtg acgtaatggc gcaaagcccg
    28681 gctgaaaaag ccggcatcaa acgcggcgac gtaatcgttg gcttcaacgg cacgaagatc
    28741 ggcgacacgg caaccctgcc ggctctagtg gcttccaccc cggtgggcaa gaccgtgcct
    28801 gtcgagatcc tgcgcgatgg caaaacgcat acgctcaagg tcgcgatcgc taaactcgac
    28861 gatcaaatcg cgacgctgga gccggagcag gagaagggcg agtggggatt gacgctgcag
    28921 aacataaaac ctgaagagcg aacggcaatg aaccttaccg gcaatgaggg ggtgctggta
    28981 gcatcggtcg ttcccggcag tccagccgcc cgcgcaaaca tcaacgccgg cgatgtgatc
    29041 ctacaggtta accgcgcttc ggttacttcg gtgcaacaga tgaaagatga aatcgccaag
    29101 agcaaaggag acaagccgct gctgctactg ttgagacgcg ccgatggcgg cacgtcttat
    29161 gccgctttgt cccgcaacgt cggataatac tgcaatccgc tcttcccgga acaacccagg
    29221 ggatgggatg gctcttcagc tagcttatcc cctgtgattc ccccctcggc aaacatagaa
    29281 gagttctccg cgcccgtatc tcttttgtac gttgcggcgg aaacgaaaaa cgagaatacg
    29341 ccattccccg accgtcggaa tagcgatggt cttcgaatat cgctacggac aattcttcca
    29401 gatgtctggt gaatttatat ggaattaaag attaagttac tcgaggcatt cgaggaatcg
    29461 tactatctca ttgtcatcgc ccaggggaaa gtcggtagcg cggcgttgaa gcggttgttt
    29521 gctgaagttg aaaagatcac cgagccactt ccggattgca aggtctttat cgatcttgaa
    29581 gaggcacgcc tcaacgtgcg acgtgaagct gttcacgcga tcgtcactca gctcgaactt
    29641 cattcaaaat cccgtcttgg taagatggtg gtcgtctcct cggagttgga ccgttgcggg
    29701 cggctgcagc acgtgcgcga tctgttatgt agccggggct tcagagtggc cgtatttgat
    29761 gacaccacaa gtgccgcgga atggttgagc gagggaacgt agtttcaagc gaaccggccg
    29821 acaggtgctt tcttcgacgc tgtctcggat caagaaacat tgccgatgcc cagcaaacac
    29881 gtcgctgtct tctcctggct ctcatcactt ggtgagaagt tctaaccgat cgaccctcag
    29941 ttcgctcctg attgttcgct atgaagatgc gtgtgaactt tcggattgac gggagcgaca
    30001 aaattgaaat aagagagacg gatatgctaa ttggaccggt atccaaggag caactgcgat
    30061 gcacgctcga ttacgacatc gacagataca gaggagggag agtggactgg ttacttcaaa
    30121 cgactgactc gttggcctat gtgttcttac gttttggctt ggcggtgacg tttttttttc
    30181 acggcaccca gcatgcgttc ggctggcacg gcggcaaagg catgaaagag cagattgcta
    30241 actggcataa caaatatcac attcctgttg ctatcggcat gttcggcaca ttcgtcgaac
    30301 tgtttactgt tttcgccatg gcccttggtt tcctggtgcg gcctgcggct ttttttctgg
    30361 cgattttcat cgctacggct atgttcaaaa gccactgggg tcacggtttt tttctacagc
    30421 aaaaaggggg aggaagcggt atcgaatata ctcttgcgct gctgctcatg gcattggcgg
    30481 ttctgtttgg cggcgccggg gcgttctcga tcgaccggat gatcatccgc tagaattccg
    30541 tcctagtagg agttgcccgt gcatgcacag atctttgtag tttttattta acttgtaaat
    30601 cgataattta ttagccgacc gtttgaaagg agaggcacgg atggaaatag gcggaagaaa
    30661 gagcgatctc tcacgacgtg atttggtcac caccacggca accgcagatg acaccgatga
    30721 gagcttcgga cccggcacgt tgggcgcccg ctatgggaat gtgccgcaga gctgggattt
    30781 tgaagccgac gtggtcgtga tcggctcggg cgcggccggt cttcccgcgg ccattcgcgc
    30841 ggtcgagtcg ggcgcttcag tgattgtagt ggaagcgaat tacgatgtcg gcgggcacgc
    30901 cattttgagc ggcggcaatg ttccactcgg cggcggcacc agcgcccaga gaaaattcgg
    30961 catcgaagat tctcccgacg tggtttttag cgatttgacg gactggtcga tcgtgcagcc
    31021 gaacggctgg ccggactatc gctataacga tcgtgaggtg atgcgcgctt tcgcggatca
    31081 ctgcgcgccg acgtacgagt ttctcgttgc caacggcgta gaattcaagg acatcgcgcc
    31141 ggacaatcaa ggcgcgcaca ccacgggcaa ctcggcgccg cgggaaaatc atgcgttctg
    31201 gaaaaagggc gcgggaccgg aaagccccaa tgcccgcccg ggaaccggtc tcatccggcc
    31261 gctcgaggca agcgcgcgca agaagggcgt aaagttcctg ttgaactata agatgacaag
    31321 cttgattcgg gagacgcagg gcgcgcgccg tgtggtcggg atgacagcct actacaggcc
    31381 gcggattatg ccgggtgaat cggcgccgct gaaaagcttc cgcagcgacg gcaatattaa
    31441 cagcaccaag ccgaacgtga acattcgcgc caagaaggcg atcgtcgtcg ccacgggcgg
    31501 gatgaccagc aatttgcatt ttcgccgcat gttcgacccg cgtcttaccg acgtgctgca
    31561 ggtagccggc gag
//
LOCUS       DASUVS010000070         8419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1335935, whole genome shotgun sequence.
ACCESSION   DASUVS010000070 DASUVS010000000
VERSION     DASUVS010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8419
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335935"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /gene="tcuA"
                     /locus_tag="VF182_04155"
     CDS             <1..1190
                     /gene="tcuA"
                     /locus_tag="VF182_04155"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX6436298.1"
                     /translation="YAADKKLTNILVDRSYDTARWLTDMNVKWILSTSTHAVKMGGKI
                     KFPSGRVISVNDGGLGLVEMLFPTAEKNGIEIIYEAKATALIVDPRGRVSGVRVQTAD
                     GLMDFQSRAVVLAAGGFEANPEMRARYLGTGWDLVKVRGSRYNTGEVLSLALSFGAQA
                     VGHWSGCHAVLVDAEAPDVECAYEHRYSYPYGIMVDINGSRFVDEGEDFFSYTYAKCG
                     REVLRLPWRTAYQIFDSKTRPLLRSEYNRGFHVSADTIEALAKKLPGLDWENVVKTVN
                     AFNDAVNDAPFDPSKHDGKSTSGISPVKSNWAQRLDSPPYYAFPVTCGITFTFGGVGI
                     TENGEVKDTSGNVIGGLFAAGEITGGSFFNNYPGGSGLMKGAVFGRLAGTSAAAYAKA
                     NRV"
     gene            1362..2378
                     /locus_tag="VF182_04160"
     CDS             1362..2378
                     /locus_tag="VF182_04160"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6436299.1"
                     /translation="MNSRRNLISFIAIAIFVVFFAQSVPAQDRDAIIAKAKEEKVFVY
                     YSTTDIRDGTAMIHAFQKKYPFVEPKLLRLGSTQVVVKVLQEHRGGVHLFDVISATSF
                     QFYEIFKEGLFQMYNSPERPAFADDFKDKEGYWVSAYHNASVVSYNTNLVKPQELPKS
                     YDDLLDPKWKGKMLMDSRETEWYASILQILGREKGLRLMRGFAKQELSFRPGRTLITQ
                     VLASGEAPLAVNNYDHLVQSAKKRGAPVESLPLTPVVSRVTPIGLGRYAPHPNIGKLF
                     IDFSLSEEGQKILRSFGRSSARKGIEPDDLQRKGIRLYVSDISLAKDYARYDKEFKEI
                     FDLK"
     gene            2451..3812
                     /locus_tag="VF182_04165"
     CDS             2451..3812
                     /locus_tag="VF182_04165"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6436300.1"
                     /translation="MTSGITEQLAIHTARVRYEDIPPEAIAKAKDCILDQIGVELIGS
                     TLEWNKIAYRYVAEMGGRGESTVVNYGIKVPTLDAAFVNATFGQGCELDDVGFAAGGH
                     PSAATVPVALALGEKQRSTGKDFLAAVVVGCDVMYRLLAAVRPSSGKRGFHSHGIGAP
                     FGAMATAARLLGLKEGQMLHAFGIAGMHSSGTMEYDQTGGEVKRVIAGVGARSGIQSA
                     MLAQLGLTGPPTIIEGKRGFLRVFADLVEPEEITRGLGVEFKIMRIWFKLYPCVATVQ
                     GVIYTLSKLIEEHGFGADDVEEIRVGISETSLSHGGAIYEPHDTASAQFSLPFSLAIR
                     LLKKDNDLSFYMDPKLWNDPKVLALAKKVKSYADPNAKKDQNYNTTMEVKLTDGKSYK
                     AFEPYPPGSPLNMVSRDVLRTKFRKMARAVLPEDRIDRLIDAVDRLETCNDTGELLPL
                     VVK"
     gene            4474..5976
                     /locus_tag="VF182_04170"
     CDS             4474..5976
                     /locus_tag="VF182_04170"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX6436301.1"
                     /translation="MKGAILCVSASVLSLLLIGSAMAQDVPALQELGKRIFFDNISNP
                     PRQSCSTCHEAENGWTGGVAGINKKGVAIPGANPHTVGGRKPPSNAYASFSPPFGTRP
                     ATNPNIPDPLDSCRQGLGFACFGGIFWDGRAEGNANALVAPPNVTGAGAIPHVGVEIF
                     DSEAQKSAYGGFIGPVADQALGPFPNDVEQNVPDGNDGGLPGAGAVCRHVNSAKYAEL
                     FTKAWNEPMDCGAANVVISFKRIALAIAAWQHSDEVNSFSSKRDQGIRNGGVFPLPNL
                     TSRENLGRDLFYGKNDSGLNRLVDNGSGALIPKNAGCAGCHNSHGAGSVGNELTQIYS
                     DFAYHHIGLPANFEIENFDPNNLDAGMLRHTDPSAVFNSANPDATPFAGHVKTPTLRN
                     VDKRRGNGFPKAYMHNGYFKSLEQVVHFYNTATLLRDPVACPAGTAAEQAIARNCWPV
                     PEFDTPNQAGNFGLLGNLGLTADEEAALVAYLKTLTDTVTPQQPQPYKGG"
     gene            complement(6029..6229)
                     /locus_tag="VF182_04175"
     CDS             complement(6029..6229)
                     /locus_tag="VF182_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436302.1"
                     /translation="MITDIQQAPTLFHKKTVDRTVFIRSMIGAPIPATGYPARSTKLD
                     EHCQLRAEFEARHLSAPDPCVS"
     gene            complement(6226..7161)
                     /locus_tag="VF182_04180"
     CDS             complement(6226..7161)
                     /locus_tag="VF182_04180"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436303.1"
                     /translation="MLTAVSSIEARTIHVAIPCYCFQYVPFFVAKDRGYYREEDLDVQ
                     LVVMGGGIGLRALIGGNVEFAATGEPAVLSAHLSGARLQILFSSYYKPLLWLYSDPKI
                     RSITDLKGKKIGVSGIGAATDTLMRDILKKHGIEGGREVAMLALGSPEARFLALKTGN
                     VDAAVLPEGLKFVAEEAGLRELVSFLNEDIFLLSGSVGARENLITSDRSLVEKFLRGT
                     VKGLLYTQSKRSAALPYVVRTLKVKESHAEKIFDLSLPGFTRGGFVDENLQKEAYRQA
                     VERLTIKDPPPLDRVFNYNITKNILTDIESKGWTP"
     gene            complement(7354..>8419)
                     /locus_tag="VF182_04185"
     CDS             complement(7354..>8419)
                     /locus_tag="VF182_04185"
                     /inference="COORDINATES: protein motif:HMM:NF044751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX6436304.1"
                     /translation="ELSGIKLDGDPRLNSNLNVFFSCPEFVSMQHGRPAQMHRVVGAE
                     GMWGNMHALDGKGLWRMTVQLKGDSQPPIESIIRRVAGRDVQFQVLAVYKWDRRKMIA
                     EQYRNGRVFLAGDAAHQMSTTGGFGMNTGIGDAVDLAWKLQAMLQGWAGPALLDSYEA
                     ERKPVALYNLDEATDMFWKTKALLPGDETISEDSSKGERLRELFAKTLIESNVKRQFD
                     VEGVALGYRYDPSPIIVPDGTLPPPDDVQVYTPTARPGSRAPHAWLKDGRSTLDLFGN
                     GFTLLRFGGGSSDVSNVVGDARAGRVPLTLYDIDEAPVAALYERKLVLVRSDGHVAWR
                     SNSPPENARRLIDQVRGAHA"
BASE COUNT         1930 a         2349 c         2350 g         1790 t
ORIGIN      
        1 agtatgccgc cgacaaaaaa ctcaccaaca ttctcgtcga tcgatcttac gacaccgcgc
       61 gttggctgac cgacatgaac gtcaaatgga ttctttccac cagtactcat gcggtaaaga
      121 tgggcgggaa gattaagttt ccctcgggtc gggtgatttc ggtgaacgac ggcggcctcg
      181 gtctcgtgga gatgctgttt ccgaccgcgg agaaaaacgg catcgaaatt atttacgagg
      241 cgaaggcgac ggctttgatc gtcgatccca ggggaagggt ttccggtgtg cgggtgcaaa
      301 cggcagacgg gctgatggat ttccagagcc gcgccgtggt gctggctgcc ggcgggttcg
      361 aggccaatcc ggaaatgcgc gcgcgttatc ttggcaccgg ttgggacttg gtgaaagtcc
      421 gcggctcgcg ctacaacacc ggcgaggtgc tcagtttggc gctgagtttc ggcgcacagg
      481 cagtcggcca ttggagcggc tgtcacgccg tgctggtcga tgccgaagcg cccgacgtcg
      541 aatgcgccta tgagcaccgc tactcctacc cctacggcat catggtcgac atcaacggca
      601 gccgcttcgt cgacgaaggc gaggattttt tcagctacac ctacgccaag tgcggccgcg
      661 aagtactgcg cctgccttgg cggacggcct atcaaatttt cgatagcaag acccggccgc
      721 tgctccgctc cgaatacaac cgcggctttc atgtcagcgc cgatacgatc gaagcgttgg
      781 cgaagaagtt gccgggtttg gattgggaga acgtcgtgaa aacggtcaac gcgttcaacg
      841 acgcggtcaa cgacgcgccg ttcgacccgt cgaagcacga tggcaaatcg acgagcggca
      901 tcagtccggt gaagtccaat tgggcgcagc gcctggacag cccaccctac tatgcgttcc
      961 cggtgacctg cggtatcacg ttcacgttcg gcggcgtcgg catcaccgag aacggcgagg
     1021 tcaaggacac gagcggtaat gtgattggcg gcctgttcgc cgcgggggaa attaccggcg
     1081 gctcgttttt caacaattac cccggcggtt ccggcttgat gaagggcgcc gtgttcggcc
     1141 gtcttgcggg caccagcgct gctgcctatg ccaaggcgaa ccgagtttag gcgggctgct
     1201 gaaaaaactg atatggttca tgcgctcaat cgcctgcgga attcgcgttc gtgatacgtc
     1261 ttcatgctcg tccggatcac gactacgttt tcacggacgc gagcacgatg tagacgcgcc
     1321 tttcggcgaa tggcgcaata atttgcaagg aggagtcgag cgtgaattcc cggcgtaatc
     1381 tcataagctt catcgcaata gcaatcttcg tggttttctt cgcccaatct gtcccggcgc
     1441 aggatcgcga tgcgatcatc gccaaggcca aggaagaaaa agtgttcgtg tattacagca
     1501 ctacggatat ccgggacggg acggcgatga tccatgcgtt ccaaaagaaa tatccgttcg
     1561 tcgagccaaa gctgctgcgt ctcggcagca cgcaggtcgt cgtcaaggtg ctgcaggagc
     1621 accgcggcgg cgtccacttg ttcgacgtca tctccgcgac cagttttcag ttctatgaga
     1681 tcttcaaaga aggcctgttt caaatgtaca actcgccgga gcggcccgca tttgccgacg
     1741 atttcaaaga caaggaaggc tattgggtca gcgcttacca caatgccagc gtggtgtcgt
     1801 ataacaccaa tctggtgaag ccgcaggaac tccccaaaag ctacgatgat ctgctcgatc
     1861 ccaagtggaa aggcaaaatg ctcatggaca gccgcgagac cgaatggtac gcgagcatcc
     1921 tgcaaattct cggcagagaa aaaggcctgc gcctgatgcg cggtttcgcc aaacaagagt
     1981 taagctttcg cccgggacgc acgctgatca cccaggtatt ggcctccggc gaggcgccgc
     2041 tggcggtcaa taattacgac catctcgtgc agtcggccaa gaagcgcggc gcacccgtgg
     2101 agtctttgcc gctgacgccg gtcgtcagcc gggttacccc gatcgggctc ggacgctatg
     2161 cgccgcatcc caacatcggc aagctattta tcgacttttc cctttccgag gaaggccaga
     2221 aaattcttcg tagcttcggc cgcagctcgg cacgcaaagg catcgagccg gacgacctgc
     2281 agagaaaagg catcaggctt tacgtcagcg acatcagcct ggcgaaggat tacgcgcgct
     2341 acgacaaaga gttcaaggag attttcgatt taaaatgatc aaggttgcgc gtcacgcagc
     2401 gattcactcg acgatacaat agaatcatcc ggcatacacg agagggaaaa atgacaagcg
     2461 gaataaccga acaactggcg atccacacgg caagagtgcg ctacgaagac attcctcccg
     2521 aggcaatcgc aaaagccaaa gattgcatcc tcgaccagat tggggtcgag ctgatcggtt
     2581 ccaccctcga gtggaataag attgcttacc gctacgtcgc cgaaatgggc gggcgcggcg
     2641 aaagcaccgt cgtcaattac ggcatcaaag tgccaaccct cgacgccgca ttcgtcaacg
     2701 ccaccttcgg tcagggctgc gagctcgatg acgtcggctt cgccgcgggt ggccacccca
     2761 gcgccgctac ggtgcccgtg gcgctggccc taggtgaaaa gcaacgcagc acgggcaaag
     2821 attttctcgc cgccgtggtt gttggctgcg acgtcatgta ccggctgctg gcggcggtgc
     2881 gaccttcgtc gggcaagcgc gggtttcaca gccacggcat cggcgcgccc ttcggcgcga
     2941 tggcaacggc agcgagactg ctaggattga aagaggggca gatgctgcat gcattcggca
     3001 tcgccggcat gcactcgagc ggcaccatgg aatacgatca aaccggcggt gaagtgaagc
     3061 gggtgattgc cggtgtcggc gcgcgcagcg gcatccagtc ggcgatgcta gcccaactcg
     3121 ggctcaccgg accgccgacc atcatcgagg gcaaacgcgg atttcttaga gtcttcgccg
     3181 atctggtaga gcccgaggaa atcacccgcg gtctcggcgt tgagttcaag atcatgcgca
     3241 tctggttcaa gctttatcca tgcgtcgcca ctgtccaggg agtcatctac acccttagca
     3301 aattgatcga agagcatggc ttcggggcgg acgacgtcga agaaattcgc gtcggcatct
     3361 ccgagacttc gctgtcgcac ggcggcgcga tttacgagcc gcacgatacc gcgagcgcgc
     3421 agttcagcct gccgttctca ttggccatcc gcctgttgaa gaaggacaac gatctgagct
     3481 tttacatgga tcccaagctg tggaacgatc cgaaagttct ggctctagcc aagaaagtga
     3541 aaagctatgc cgacccgaac gcaaagaagg accagaatta caacacgacg atggaagtga
     3601 agctcaccga tggcaagagc tacaaagcgt tcgagcctta tccgcctggc tcgccgctca
     3661 acatggtcag ccgtgacgtg ttgcgcacca agtttcgcaa gatggcgcgc gcggtattac
     3721 ccgaagatcg gatcgacaga ttgatcgatg ctgtggaccg gctggaaacc tgcaacgata
     3781 ccggcgaatt gctaccgctg gtcgtgaagt gatcgtcggg ataaccgcgc gcttcccgaa
     3841 cttccgttaa gaaattactc cctggtttca ttaggttcat ctgttcgttg atcgttgtta
     3901 tcatgtggac aattcatttt ccgcgaatcc tatcggtaag taaaattgcc tataagcaag
     3961 cgcagggtat tccaaacata cgatgactcg aatttcagtc agacggtgct aagccaaaga
     4021 tctaaaatcc tcgtttcatt caccacttta aaaaattttc atcgttgaat catcttcgtc
     4081 ggagcggatt cataaatata attttacgcc gatctcttga tcactcgccc ggtgatcgag
     4141 cttgtcaccg acctttgtaa aagtatgaat gccgtcgagc gctcgatgat agtcagcgca
     4201 gtatgatcaa gaagtctttt ttttcacatg ccatggtatg gggattgctc gagacgcagg
     4261 gcgcggttct ggtaaaaatt tccattcaaa agaacagaga ttgattctgc agcagcgcga
     4321 gattctcggc gcgagtttat cgatgtgccg agcgtgcgct ggagccatgt ggaaatggat
     4381 cgactaaata aacagggggc gagatccccg ggagtgataa tgcgaatcga aaaccctgaa
     4441 aatcaagcaa caaggtaccg aagcacagct cgcatgaaag gtgcgatact atgcgtttct
     4501 gccagtgtgt tatcactgct attgatcggg tctgccatgg cccaggacgt ccctgctcta
     4561 caagaactag gcaagcgtat ttttttcgac aatatctcta atccaccgcg ccagtcatgt
     4621 tccacttgtc acgaggcgga aaacggctgg acaggcggcg tcgccggcat caataagaag
     4681 ggtgtggcga tacccggcgc caaccctcac accgtcggcg gacgtaagcc gcccagcaat
     4741 gcgtacgcga gcttcagtcc accgttcggt acccggcctg cgaccaaccc aaatattccc
     4801 gacccgctcg actcttgtag gcaaggttta ggatttgcgt gtttcggcgg gattttctgg
     4861 gacgggcgcg cggaaggcaa cgcgaatgcc ttggttgctc cgccgaacgt gaccggagca
     4921 ggagcaattc cgcacgtcgg agttgagatc ttcgatagcg aggcgcaaaa gtccgcttac
     4981 ggtggtttta tcgggcctgt ggcggaccag gcgctcgggc cgttcccgaa cgatgtcgaa
     5041 cagaacgttc ctgacggaaa cgatggtgga ttacccggtg ccggagctgt ctgtcggcat
     5101 gtcaattcgg ccaagtacgc cgagctgttc acgaaagctt ggaacgagcc gatggactgc
     5161 ggcgccgcga atgtggttat cagcttcaag cgcatcgctc tggcgatcgc tgcctggcag
     5221 cattccgatg aagtcaactc gttcagctct aagcgcgatc aaggaatcag aaacggcgga
     5281 gttttccctc tccccaattt gaccagtcga gagaatctgg ggcgagactt attctatggc
     5341 aaaaatgatt cagggctgaa tagactcgtc gacaacggta gcggtgccct tatcccaaaa
     5401 aacgcaggct gtgccggctg ccacaacagc catggcgccg gttctgtagg caatgagcta
     5461 acacagattt attccgactt tgcgtaccac catatcgggt tgccggcgaa cttcgaaata
     5521 gaaaacttcg atccgaacaa tttagacgca ggcatgctca ggcatacaga tccaagtgcc
     5581 gtgttcaact ctgcaaaccc tgacgcaact ccatttgcgg gacacgtcaa aaccccgacg
     5641 ctgcgcaatg tcgacaagcg ccgcggcaac ggcttcccta aggcgtacat gcacaacggc
     5701 tacttcaagt cactagagca agtagtgcac ttttacaaca cggccacctt gttacgcgat
     5761 cccgtggctt gtcccgccgg tacggcggca gagcaggcaa tcgcaagaaa ctgctggcca
     5821 gttccagaat ttgatacccc caatcaagct ggaaatttcg gcctgctggg aaatcttgga
     5881 ctgacagcgg acgaagaggc agcgcttgtg gcatacttaa agacgctgac cgataccgtg
     5941 acgccccagc agccccagcc ctacaaaggc ggctagggaa atcggcgttg ccgaaagttg
     6001 ttcgacccag acgggggttg cggcgttgtt aactgacaca aggatccggc gcagaaagat
     6061 gccgcgcctc gaactcggcg cgcaactgac aatgctcatc cagcttggtc gagcgagcag
     6121 ggtagccggt tgccggtatg ggtgcgccga tcattgaacg gatgaaaact gttcgatcga
     6181 ccgttttctt gtggaacagc gtgggggctt gctggatgtc ggtaatcacg gtgtccagcc
     6241 tttcgattca atatcagtca aaatattttt tgttatgttg tagttgaaga ctctgtctaa
     6301 gggaggaggg tcttttatgg ttagacgctc gacggcttgg cggtacgctt ccttttgaag
     6361 gttctcatcg acaaatccgc cgcgggtaaa ccccggtaac gacaaatcaa agattttctc
     6421 tgcatgactt tcttttacct tcagggtacg cacgacgtac ggcagagcag ccgaccgctt
     6481 ggattgcgta tagagtaatc cttttactgt tccgcgaagg aatttttcca ctagacttcg
     6541 atcgctcgtg atcaaatttt ctcgagcgcc gacgcttccc gacaacaaga agatatcctc
     6601 attgaggaag gagaccaact cacgcaaccc cgcttcctcg gcgacaaact tcaacccttc
     6661 tggaagaacg gcggcatcga cgttacccgt cttgagcgcg agaaagcgcg cttctggaga
     6721 tccgagcgca agcatcgcca cttcgcggcc tccctcgatg ccatgcttct tcaggatgtc
     6781 gcgcattaat gtgtcggtcg cagccccgat tcccgagact ccaatctttt ttcccttcag
     6841 gtcggtgatg gacctgatct ttggatcaga gtacagccag agtaacggct tatagtagct
     6901 gctgaaaaga atctggagtc gcgcgccgct gaggtgtgcg gacagtacgg caggctcacc
     6961 ggttgccgcg aactctacat ttccaccgat aagggctcgc agtccgatac caccacccat
     7021 tacaaccaat tgcacgtcta aatcttcttc tcgataatat cctctgtcct tggcgacaaa
     7081 gaaaggcaca tattggaaac aataacacgg tatcgcaaca tggatggttc gagcctcgat
     7141 tgaagatacg gccgtcagca ttgctccgaa gcaaattgcg gcatatcgca atgattttgt
     7201 tatcatgttg gtacctgcac ttttatttgg ataattccag gtaacggacg attaaaaaag
     7261 cagtaattgg tgtgtctcga atccggccgc agctactcta attagatgat tgctcgttga
     7321 gtcatacgtt ctagccggca ttgatccttt ttatcaggcg tgcgcgccgc gcacctgatc
     7381 gatcaatctc cgcgcgttct cgggcggaga attggaacgc cacgcgacgt gaccgtcgga
     7441 gcgaaccagg actaacttgc gctcgtagag agctgcgacg ggtgcttcat cgatatcgta
     7501 aagggttaat ggcacgcggc cggcccgcgc gtctccaacg acgttggaga cgtcggaaga
     7561 accgccgcca aatcgcaaaa gcgtaaatcc atttccgaaa agatccaacg tcgatcgccc
     7621 gtccttcagc cacgcgtgcg gcgcgcgcga acccggacga gccgttggcg tatagacctg
     7681 tacgtcgtcg ggcggcggca gcgtgccgtc cggcacaatg atcggagacg gatcgtagcg
     7741 gtaacccagc gctacgcctt cgacatcgaa ttgccgcttg acattgctct cgatgagcgt
     7801 tttcgcgaat agctcgcgca agcgctcgcc tttcgatgaa tcctccgaga tggtttcatc
     7861 gcccggcagc agcgctttgg ttttccaaaa catgtcggtg gcctcgtcga gattgtagag
     7921 cgcgaccggt tttcgctcgg cctcgtagct atccagcagc gccggcccag cccacccttg
     7981 cagcatcgcc tggagcttcc atgcgagatc gaccgcatcg ccgatgccgg tgttcatgcc
     8041 aaaaccgccg gtggtcgaca tctggtgcgc ggcatctccg gccaaaaaca cgcggccgtt
     8101 tctatactgc tcggcgatca tcttgcggcg atcccactta tagacggcca gcacctgaaa
     8161 ctgcacatcc cgaccggcca ccctccgaat gatagattcg ataggtggtt gagagtcgcc
     8221 tttcaattgc acggtcatac gccacagacc cttgccgtcc aaggcatgca tgttgcccca
     8281 catgccttcg gcgccgacaa cccgatgcat ctgcgccggt cggccgtgtt gcatcgaaac
     8341 gaattcaggg cagctaaaaa atacgttcaa gttcgaattg agacgcggat ccccatccag
     8401 tttgattcct gaaagttct
//
LOCUS       DASUVS010000071        14202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1554375, whole genome shotgun sequence.
ACCESSION   DASUVS010000071 DASUVS010000000
VERSION     DASUVS010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14202
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554375"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VF182_04190"
     CDS             <1..384
                     /locus_tag="VF182_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009625546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HEX6436305.1"
                     /translation="APAPLPNGNLLIFDNGPHRLDHALPFSRVIEVEPGTKQIVWEYR
                     EKRESDFFSPRISNAQRLPNGNTLICEGDFGRLFEVTAGGEVVWEYVNPYFGGPSNAQ
                     HNRVFRAYRYSAEEIAKARAAAGAA"
     gene            complement(569..1048)
                     /locus_tag="VF182_04195"
     CDS             complement(569..1048)
                     /locus_tag="VF182_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEX6436306.1"
                     /translation="MVRISWLASAILFNFFMHVSAMAQTLEVKRYNDAEWTKGRFTEV
                     VTVTGPGKMIFLAGVGAEDEDKPQGAIRHLGDPYLQCKYAYDKMKRHLAKHGGTLADV
                     VKQVVYVTDIRYQADVGKCRREEYGEKGPIPANTFLVISGLAIPGMLLEIDITAVVP"
     gene            1622..2809
                     /locus_tag="VF182_04200"
     CDS             1622..2809
                     /locus_tag="VF182_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEX6436307.1"
                     /translation="MSKVRVLVGTRKGAFLLTADAKRESWNVSGPHFAGWEIYHIKGS
                     PADPDRLFASQCSSWFGQIIQRSDDGGKTWHQPGTPPGEPTTTPDGMPKGESNKFVYD
                     SSPETGKPLTTHQWYDGTPKPWEFKRVWHLEPSLNDPEVIYAGVEDAALFRSINGGQT
                     WHELAGLRGHGTGSAWQPGAGGMCLHTILLDPSDPNRIYIAISSAGAFRTNDAGKTWQ
                     PINRGLHSLYIPEPTAEIGHCVHRIAMNPSRPGVLFMQKHWDVMRSDDAGESWREISG
                     NLPTDFGFPIDVHAHEPDTVYVVPIKSDSEHFPPDGKLRVYRSRTGGNEWEALTKGLP
                     QSDCYVNVLRDAMAVDSLDSCGVYFGTTGGQVYASSNAGDSWAPIVRDLPPVLSVEVQ
                     TLP"
     gene            2966..3262
                     /locus_tag="VF182_04205"
     CDS             2966..3262
                     /locus_tag="VF182_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX6436308.1"
                     /translation="MIRVTLPAHLRKLAHIDGEVSLEIAGQITQQSILDALEARYPML
                     RGTIRDHVKGKRRAFVRFFACGEDLSHELPDTPLPDAIATGAQPFMILGAIAGG"
     gene            3395..3814
                     /locus_tag="VF182_04210"
     CDS             3395..3814
                     /locus_tag="VF182_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015174426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6436309.1"
                     /translation="MAMKIFVNLPVKNLNRSVEFFSKLGFSFDKQYTDETATCMVVSE
                     EIFVMLLTEEKFQTFTPKQICDAAKFTEVLLCLSLESRAKVNEMAAKAVAGGGSTYND
                     PQDHGFMYAHGFQDLDGHIWELIYMEPTAVKEPHKGD"
     gene            3880..4437
                     /locus_tag="VF182_04215"
     CDS             3880..4437
                     /locus_tag="VF182_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013818772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6436310.1"
                     /translation="MIKILMIMPVVVIMFAGIVALRPSIFHVARTLKMSATVPAVFAQ
                     VNDFHNWKAWNPWGKLDPAMQQTYQGAPAGTGAVYTWAGNKEVGEGRMTIIESRPNDL
                     IRINLEFFKPFAATNLAEFTFRPEGDQTAVTWSMSGKNKFIAKGIHLFMNMDQMIGGP
                     FERGLAQMKSVVEAASNTSEMTFND"
     gene            4549..4896
                     /locus_tag="VF182_04220"
     CDS             4549..4896
                     /locus_tag="VF182_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX6436311.1"
                     /translation="MKYKGSCHCGRIVFEAEGEIKGATACNCSICSRKGSLLWFVPRE
                     KLTVLTAEEAAGTYTFNKQVIKHHFCQTCGIHPYGEGVDPKGNKIAAINLRCVEGIDL
                     KSIPVQNFDGASL"
     gene            4932..5339
                     /locus_tag="VF182_04225"
     CDS             4932..5339
                     /locus_tag="VF182_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6436312.1"
                     /translation="MDLLVNLDVDDLDKAVRFYSSVFGLRVGRSLGAAGLEMLGCSSP
                     IYLLVKSATLPAETTSQVRTYRRHWTPVHLDFVVDEIEPAIKRAVLAGALLEKPVQVF
                     EWGKLALMADPFGHGFCFVQFVGRGYDAIADSV"
     gene            5418..5816
                     /locus_tag="VF182_04230"
     CDS             5418..5816
                     /locus_tag="VF182_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015237243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX6436313.1"
                     /translation="MNVALWIAQVLLAVIFLFAGGMKLVMPIEELTAQMPMPGLFVRF
                     IGVVEVLGGLGVILPWLTGIRPGLTPLAAAGLVIIMIGATVVTVMIVNIAMALIPLVV
                     GLLAVFVAYGRWRLTAHRESTCGSVLQPGR"
     gene            5909..6205
                     /locus_tag="VF182_04235"
     CDS             5909..6205
                     /locus_tag="VF182_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX6436314.1"
                     /translation="MCRNIKVLHNFEPPVTEAEIRAAALQFVRKISGFNTPSKVNQKA
                     FSAAVDEVARISATLLASLESTASPKNRQDEAAKRKASAVRRFDRQVPMEHRDD"
     gene            6354..7199
                     /locus_tag="VF182_04240"
     CDS             6354..7199
                     /locus_tag="VF182_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX6436315.1"
                     /translation="MIVHLIDGTYELFRHFYGLRRFNKGRDRPFGAVIGVLNTVLQMI
                     ESGATHVGVATDHVIESFRNRLWPDYKTGDGIEPALLAQFHPLEEALAAMGVAVWPMI
                     ELEADDALASAAQIASGNAAVEKICIWTPDKDLAQCVRGDRIVQVERRTQKMRDAEGV
                     REKFGVDPVLIPDLLALIGDAADGFPGVPGIGPVTAARLLNRHGMIENFPSTVLGNNR
                     ELALLFKNLATLRTDACLFRDIEELRWRGPSKGFAAWVERLGDSRLLERCLKAESMIS
                     IADLA"
     gene            7231..7614
                     /locus_tag="VF182_04245"
     CDS             7231..7614
                     /locus_tag="VF182_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEX6436316.1"
                     /translation="MRYVDGFLLPVPKKNLQAYRRMAEKAGKIWREHGALEYRECAGD
                     DLNVKGLVSFPRTIKLKRGETVVFAWIVFKSRSDRDRVNAKVMKDPRLADSMDPKSMP
                     FDVERMVYGGFNVLVDELSSSGNGR"
     gene            complement(7700..8107)
                     /locus_tag="VF182_04250"
     CDS             complement(7700..8107)
                     /locus_tag="VF182_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6436317.1"
                     /translation="MATIQKEIAIERSKEFVWDAIRDLGAIHKRLVPGFVVDCKLEGG
                     WRTVTFANGMVVRELIVAIDDKTCRHAWSARGAPLTHHNASVQVFADGDDKCRVVWIA
                     DLMPNEVADTIGKMIQQALNRMKQTLEGITTAH"
     gene            complement(8150..8527)
                     /locus_tag="VF182_04255"
     CDS             complement(8150..8527)
                     /locus_tag="VF182_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6436318.1"
                     /translation="MPIVLDHTIVPAKDKLASAQFFAEIFGLNVKPGDGYFAQVQVND
                     NLTFDFADETEPQSHHYAFHISDAEFDAIYQRVKSKGLPYGSGPYNHTDGKIYTRRGG
                     RGFYFEDPNGHLLEVMTVPETGS"
     gene            complement(8605..8979)
                     /locus_tag="VF182_04260"
     CDS             complement(8605..8979)
                     /locus_tag="VF182_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016557908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6436319.1"
                     /translation="MSKVSVRYIVDDVDAAIPFYTSMLGFKLEMHPAPGFASLSRGDL
                     QLLLNRPGAGGAGQAMPDRELPSAGGWNRIQIEVDDLEATVEKLREAGARFRNEIVTG
                     NGGKQILVEDPSGNPIELFQPF"
     gene            complement(9101..9676)
                     /locus_tag="VF182_04265"
     CDS             complement(9101..9676)
                     /locus_tag="VF182_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6436320.1"
                     /translation="MKLYYNPKSRAVIAKWMLDECGAKYEIVPIEFEKGEHKTPEFLK
                     INPAGKLPALHDGESKVFETAAICLYLGDKFGEVKLAPKIGAPERGRYLSLMVYSTAQ
                     LEPALGDVMLGGQTTPQRGWSDFETVRNLIEGELGSGPYLFGEWFTAADVMIGSMFMW
                     RRMLGGKPDRPKLEAYVDRLIARPAAPKLGA"
     gene            complement(10084..10500)
                     /locus_tag="VF182_04270"
     CDS             complement(10084..10500)
                     /locus_tag="VF182_04270"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX6436321.1"
                     /translation="MMQSLRAIEGGCLCGAIRYRASGNGYGITHCHCRTCRRASGAPL
                     VTWAGFDSDKFNFTQGQPVCYASSKNVVRTFCGRCGTALTYRRLDLPESVDLTLGSMD
                     DPEQLEPQNHTWTESKLSWIRLGDDLPAYPQEKKAN"
     gene            complement(10949..11149)
                     /locus_tag="VF182_04275"
     CDS             complement(10949..11149)
                     /locus_tag="VF182_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436322.1"
                     /translation="MRMIVLLALSFSLAGCATERTILVNSQGDLLTCETSGSGFFGAI
                     SVRNQQKQCISEAETRGYRVKD"
     gene            complement(11173..11925)
                     /locus_tag="VF182_04280"
     CDS             complement(11173..11925)
                     /locus_tag="VF182_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEX6436323.1"
                     /translation="MQLEQFAAQIRSYRDIVFFTGAGISTESGVPDFRSPGGVWTQYQ
                     PVYFQDFLASEAARIQHWRLKKATYELFKSVKPNIGHYAIRDFEQRGQLLGLITQNID
                     GLHKLAGVCDEKLVELHGTDRLVTCLKCGKRFEPADVYEGLAEEFSAPTCDACGGFLK
                     SANISFGQQMPAEPMRRAQIWTEAADVFIVVGSSLQVQPAASFPVIAKRNGALLAIVN
                     RDETPLDDIADYVYHGGIGVWFEQLSPLLTDA"
     gene            12254..12991
                     /locus_tag="VF182_04285"
     CDS             12254..12991
                     /locus_tag="VF182_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010062452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436324.1"
                     /translation="MISSAFQLDLAPTGKLRAGINYGNPVLARRDPTSGQLGGIAVEL
                     ARELGRRTDLPVELVGFESAGKMVEGVQAGAWDVAFLAVDSGREHEISFTAPYIEIEG
                     TYLVPPGSALSAIADVDRENVRVGVSSKSAYDLFLSRNLKQAQLVRASTPEAAFELIV
                     AGKVDVLGGVRQHLVANSVKLPGARVLDGRFMAIQQALGILKGRQAGLSYLREFIEDV
                     KASGFVAQALEKAGVQGVSIAPRMISK"
     gene            complement(13062..14042)
                     /gene="bioB"
                     /locus_tag="VF182_04290"
     CDS             complement(13062..14042)
                     /gene="bioB"
                     /locus_tag="VF182_04290"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX6436325.1"
                     /translation="MDFGKLADKSLQGELLERDEMKAVLNASDEQIPELVSAAFKVRH
                     HYFGKRVQIHMLQNAKSGLCPEDCHYCSQSSVSEAPIERYPFLSKEQLVEGAKRAKEA
                     GAVRYCIVNSGRGPTPKEIEQIAEAVKEIRAKVGINICCSLGLMSEDKIRSLKEVGVG
                     RINHNLNTSREHHPEIVTTHTYDDRVATVENVKSAGVGTCSGGIIGMGESDDDIINLA
                     VTLRAMDIESIPVNFLHSIPKTPFEAKRQLTPQKCLKTLCLFRFVNPSKEIRVAGGRE
                     VNLRSLQPLSLYPANSIFVNGYLTTPGQQASDAHRMIEDLGFELDQPASI"
BASE COUNT         3157 a         3748 c         4026 g         3271 t
ORIGIN      
        1 gcgcctgcac ccttgccgaa cggcaacctg cttattttcg acaacggtcc gcatcggctg
       61 gatcatgcgc tgcctttttc ccgggtcatc gaggtcgagc cgggaaccaa gcaaatcgtc
      121 tgggaatatc gggaaaagcg agaaagcgat tttttcagtc cgaggatttc caatgcgcag
      181 cgcttgccca acggcaacac gctgatctgc gaaggcgatt tcggtcggtt gttcgaggtg
      241 accgcaggtg gagaggtggt ttgggaatat gtgaatccct attttggcgg accgtcaaac
      301 gcgcaacaca atcgagtatt ccgcgcctac agatacagtg cagaggagat cgccaaagcg
      361 agagccgcgg ctggtgctgc ataaaaattt ttcctcgaga agtgaaacaa gtcggaacgg
      421 gaagtagatg gaatcggttg cctgggacct gcggagtccg acgttcgtaa aactcgcccg
      481 gatcagccgc agcttagtcc tgtactcatg tttcttcaaa gaggctcctg gcctttgaag
      541 cgcggcgtga tacgaacggt tccctttact acggcaccac tgcggtaatg tcgatctcta
      601 atagcattcc cggaatcgca agtccggaga tgacgagaaa agtgttggcg ggaatcgggc
      661 ccttttcacc gtactcttcg cgccggcatt tgccgacatc ggcctggtat ctgatatcgg
      721 tgacatagac tacctgtttt acgacgtcgg cgagcgtgcc gccatgcttg gccaagtgac
      781 gcttcatttt atcgtaggcg tatttgcact gaaggtaggg atcgccaagg tggcggattg
      841 cgccttgagg cttgtcttca tcctcggctc cgactccggc taggaagatc atttttcccg
      901 ggccggtgac ggttaccact tcagtgaaac ggcccttggt ccactcggca tcgttataac
      961 gcttgacttc aagcgtctgc gccatcgcgc ttacatgcat gaagaagttg aataggatcg
     1021 ccgacgcgag ccatgaaatt cttaccatgt tcgactcctc cattaacttt agctggcgta
     1081 tctctactgc tttccaatag acgcgatcgt gaagccggtc aagtgtattt tacgaagaga
     1141 tcaggagcct agtaacaacc ctggcgtagg cacagatttc ttgaaatggg aggcaacaat
     1201 tcgtgtgccc acgcaagcca cttaccggaa agaacgaaat agcagatttt ggcatttctg
     1261 tatgaactgc aaagaatggc cggacaagga tttcgacgag atcagcagcg cggcggatcc
     1321 gcattggatc agctctgcgc tgaatgtgcg gagctggaat ggaaagaggc aaaaagtgcc
     1381 aatgttccga tgaaatcgta ccctagcgcc tgagttttct ggtatctccg cttgcggaga
     1441 actcatctaa atttaaaaat tttcttgcgc cgatgtcctg aattcctcgg tcggttcgtc
     1501 gtagtattga agcaatcaac gatcgattac tctgaatagc gatcagaatc gcccgtatag
     1561 ggttgacaat agttgggaag aaactacgag ttaaactaga ttaactgaaa aggagtaagg
     1621 gatgagcaag gtacgagtgc tggttggtac aaggaaaggg gcgtttcttc tgacggcgga
     1681 cgcaaagcgc gagagttgga atgtcagcgg cccgcatttt gccggctggg agatctatca
     1741 catcaaggga tctccggctg atccggaccg attgtttgca tcgcagtgca gcagttggtt
     1801 cggtcaaatc attcaacgct cggatgacgg cggcaagacc tggcatcaac ccggaacgcc
     1861 gccgggcgag ccgaccacca ctccggacgg catgcccaag ggcgagagca acaaattcgt
     1921 ttatgactcg tcccctgaaa ccggcaaacc gcttacgaca caccaatggt acgacggcac
     1981 accaaaaccc tgggagttca agcgcgtgtg gcatcttgag ccttcgctaa acgatccaga
     2041 ggtcatctac gcgggggttg aagatgccgc cttatttcgc tcgatcaatg gcggtcagac
     2101 gtggcacgag cttgccggat tgcgcggcca tggcacaggt tcagcctggc agcccggcgc
     2161 cggtggaatg tgcctgcaca cgatccttct tgatccgagc gatcctaatc ggatctacat
     2221 agcgatatcg tctgcgggcg cgtttcgaac caatgatgcg ggcaagactt ggcagccgat
     2281 caatcgcggg cttcattcgc tctatatccc cgagccgacg gcggagatag gccactgtgt
     2341 ccaccgaatt gcgatgaacc cgtcgcgccc cggggtgttg ttcatgcaga aacattggga
     2401 tgtgatgcgc agcgacgatg ccggtgagtc atggcgcgaa atcagcggca acctgccaac
     2461 cgacttcggc ttccccatcg acgtgcatgc gcacgagccg gataccgtct acgttgtgcc
     2521 gatcaagagc gactcggagc acttcccgcc tgacggtaaa ctgcgcgtgt accgcagccg
     2581 tacaggcggc aacgagtggg aggctctgac caaaggattg ccgcagagtg attgttacgt
     2641 caacgtgctg cgcgatgcca tggcggtcga ctcactcgat tcctgcggcg tttatttcgg
     2701 caccaccggc gggcaggtat atgcatcgtc taacgcgggt gacagctggg cgccgatcgt
     2761 ccgagatctg ccgccggtac tctcagtcga ggttcagaca ctgccatgaa gcgggcaatg
     2821 aagataacct ccggcggtgt tctggcatcg aaaacgtctt caatgtagtt cagcactgcg
     2881 cctcaggcgc tttgtttgac tgtgcctggc gaaggggttt gagtactcgc gagagccgtg
     2941 cccttagctt taggagagta aagtcatgat tcgagtcacg ctcccagcgc atctccgcaa
     3001 gctcgcccac atcgacggcg aagtctcgct cgagatcgcg ggtcagatca cgcaacagtc
     3061 aattctcgac gcgctcgaag cgcggtaccc gatgttacgt ggcactattc gagaccacgt
     3121 caaaggtaag cgccgcgctt tcgtgagatt cttcgcctgc ggcgaggacc tttctcacga
     3181 attgccggat acgccgctgc cggacgcgat cgcaaccggg gcacagcctt ttatgatcct
     3241 tggcgccata gcgggcggtt agctgagccc ccgagcgggg ctcgggatta tgattattac
     3301 gccaatgcgc ctcggcgctt ggaaccgcgc tgtccggtcg aatcggttca tgccgtttgc
     3361 tgtcggcagc gcataagccg taaaaggaga aaacatggca atgaaaattt ttgtgaatct
     3421 accggtcaag aacctaaaca ggtcagtgga gtttttcagc aaacttggct tcagttttga
     3481 caagcaatat accgatgaga ccgccacgtg catggtcgtc agtgaggaaa tctttgtcat
     3541 gttgttgact gaagaaaagt ttcagacctt cactcccaaa cagatttgcg acgcggctaa
     3601 atttaccgaa gtgctgctgt gcttatctct ggagagccgt gcgaaagtga acgaaatggc
     3661 tgcaaaggcg gtcgctggcg gaggctcgac ctacaatgat ccgcaagacc atggtttcat
     3721 gtatgcccac ggatttcagg atctggatgg ccacatttgg gagctgattt acatggagcc
     3781 gaccgcggta aaagagccgc ataagggcga ttaatcagcg taccaggacc taaacgtgta
     3841 tccaactgtt gcgcaggtgc gcagcgcgaa ggattgatta tgattaaaat tctcatgatt
     3901 atgccagtcg ttgtcatcat gtttgccggc atcgtggctc tgcgaccttc catatttcat
     3961 gtcgcgcgga cgcttaagat gagcgcgact gtgccggcgg tctttgcgca ggtcaatgat
     4021 ttccataatt ggaaagcatg gaacccgtgg ggaaaattgg atccggcgat gcagcaaaca
     4081 tatcaaggcg cgcccgcggg aactggcgca gtctacacct gggctggtaa caaagaggta
     4141 ggcgaagggc gcatgactat catcgagagc cgtcccaatg atctgatccg gatcaatctc
     4201 gagttcttca aaccttttgc agccacaaac ctcgcggagt tcacatttag acctgagggc
     4261 gatcagacag ccgtcacgtg gagcatgagc ggtaaaaata aatttatcgc caagggcatc
     4321 cacctgttca tgaacatgga tcaaatgatc ggcggaccct tcgagcgggg attagcgcaa
     4381 atgaaatcag ttgtggaagc ggcatcgaac acgagcgaga tgacattcaa cgattgaatc
     4441 gattgccgaa acgatgaaca gaatagtaaa tggcgccgag tttctgaaag ttgttttcag
     4501 gaaatataaa gagagtcatg aactcaaggt cggatgggga ggtctcacgt gaaatacaag
     4561 ggtagctgtc actgcggcag gatcgtgttc gaggctgaag gagaaatcaa aggagcgacg
     4621 gcatgcaatt gctctatctg ttcgcgcaaa ggatcgctct tgtggtttgt gccccgcgaa
     4681 aagttgactg tgctcacggc ggaagaggcc gccggcactt acacgttcaa caagcaggtc
     4741 atcaagcacc acttttgtca gacatgcggc attcatccat acggcgaagg cgttgatccc
     4801 aagggaaaca agattgcggc aatcaatctt cggtgcgttg aagggattga cctgaaaagc
     4861 attccggttc agaacttcga cggagcatct ttataactca cacgagcggc gcggaggcga
     4921 ctcacggcgt gatcgatctc ctggtgaatt tggacgttga tgatctcgac aaggcggtgc
     4981 gcttttacag ctctgtattc gggttaagag tcggccgcag cctcggcgcc gcaggattgg
     5041 agatgcttgg ctgttcgtca ccaatttatc ttctggtgaa atcagcaact ctgcctgcag
     5101 agacaacttc gcaggtgcga acttaccgac ggcattggac gccggtgcat ctcgattttg
     5161 tcgttgatga aatcgaaccg gcgattaagc gagctgtgct agcgggagcg ttgctggaaa
     5221 agcctgttca agtattcgag tggggcaagc tcgcccttat ggctgatccg tttggccacg
     5281 gattttgctt cgtgcagttt gttgggcgcg gctatgacgc gatcgcagat tccgtgtgac
     5341 ggaatttcaa ccgaagctac cggtcgtcag cggcggccta tgcctagggc tgtcaatcga
     5401 gatggaggag gtcaatcatg aacgttgctt tatggatcgc tcaagtgttg cttgcggtca
     5461 tttttctatt cgccgggggc atgaagctgg tgatgcccat cgaggagtta acggcgcaga
     5521 tgccgatgcc gggtttgttc gtgcgattta tcggcgttgt tgaagtcctc ggtgggctcg
     5581 gtgtaattct cccgtggctg accggaattc ggccaggtct gacacccttg gccgctgccg
     5641 ggctggtgat cattatgatc ggcgcgacgg tggtcaccgt gatgatcgtc aatattgcga
     5701 tggcgttgat tccacttgtg gtgggactcc tagcggtgtt cgtcgcctat ggccgctggc
     5761 gcctgacggc gcaccgcgag tcgacttgcg gatcggtgct ccagccgggc cgctgaggat
     5821 cgcttctgat tccggtgcca cacttatctg taacttttta aatcaactgg agagagggca
     5881 cattcgattg cccggtacgc tgcaaagtat gtgtagaaac attaaggtcc ttcataattt
     5941 cgaaccgcct gtcaccgagg cagagattcg cgccgccgcg ctgcagttcg ttagaaaaat
     6001 cagcggcttc aacacgcctt cgaaagtcaa tcaaaaggcc ttctctgccg ctgtcgatga
     6061 ggtggcccgg atctcggcca cgcttctggc ttcgctcgaa tcgacggcat caccgaagaa
     6121 tcgccaagac gaggctgcca aaagaaaggc gagtgctgtg cgaagattcg accgccaagt
     6181 cccgatggaa catcgcgacg attgagcagg ggcacttcaa ccaagagtcc cgattccggg
     6241 agatcttcgc tactgagttc cacagtgcat tcagccggtc gaggagtaag ccaaggaata
     6301 cgagctacac tttacgcttc tattacgtca gcggtgcaag aagccacttc accatgatcg
     6361 ttcatctcat agacggcaca tatgagctgt tccgccactt ctatggcctg cgccgtttca
     6421 acaagggtcg ggaccggccg ttcggtgccg tcattggcgt cctcaatacg gtactgcaga
     6481 tgatcgagag tggagcgact cacgtgggtg tggcgaccga tcatgtgatc gaatcctttc
     6541 gaaatcgtct ctggcctgat tataagaccg gtgacggtat tgagccggcg cttcttgccc
     6601 agttccatcc gctcgaagaa gcactggcgg cgatgggagt agctgtctgg ccgatgattg
     6661 agttggaggc tgacgacgcg cttgcctccg ctgcgcagat tgcctccgga aacgcggccg
     6721 tggagaaaat ttgcatctgg acgcccgaca aagatttggc gcagtgcgtc cgcggcgacc
     6781 ggatcgtgca ggtggagcgt cgaacgcaga agatgcgcga tgccgaagga gttcgcgaga
     6841 agttcggggt agatccggtt ttgatccccg acttacttgc tctgatcggc gatgcggcgg
     6901 atggctttcc gggcgttccc ggaatcgggc cggtcacggc tgcacggcta cttaatcggc
     6961 acggcatgat agaaaatttt ccatctaccg tactgggaaa taatcgcgag ttggcgctgt
     7021 tgttcaagaa tctagcgacg ctcaggacgg atgcgtgtct gttccgcgac atcgaagagt
     7081 tgcgctggcg gggacctagc aagggatttg ccgcctgggt ggagcgtctc ggagactctc
     7141 gtctgctgga acgctgtctc aaggcagaaa gcatgatttc gatagcggat ctggcttaat
     7201 gaatgatcca atcacgacgg aggacgataa atgcgctacg ttgatggttt tctattgccg
     7261 gtgcccaaga aaaatctgca ggcatatcgc cgcatggccg aaaaggctgg aaagatctgg
     7321 cgcgagcacg gcgcgcttga gtatcgcgaa tgtgccggtg acgatcttaa tgtgaagggc
     7381 ctggtatcgt ttccgcgaac gatcaagctt aagcgcggtg agacggtggt gtttgcctgg
     7441 atcgtattta aatcacgaag cgatcgggat cgggtcaatg caaaggttat gaaagatccg
     7501 cgtttggcag attcgatgga cccgaagtcg atgccgttcg acgttgagcg catggtctac
     7561 ggcggattca acgtcttggt cgacgaatta tccagcagcg ggaacggccg ttgatatacc
     7621 gaacccaccc gatttcggct gttgaaacac aagatttacc cgatgtactg gtgccttgta
     7681 cagccttcct tgggcaaagt cagtgagcag ttgtaatgcc ttcgagcgtt tgtttcattc
     7741 tgtttaatgc ctgctggatc attttcccga tagtatcggc cacttcattg ggcatcaaat
     7801 ccgctatcca aacgacgcga cacttgtcgt cgccgtccgc gaatacctgc acggatgcgt
     7861 tatggtgagt gagaggcgcg ccgcgagccg accaagcgtg gcggcaggtt ttatcatcga
     7921 tagcgacgat gagttctcgc acgaccatgc cgttggcaaa cgttacggtt cgccagccgc
     7981 cttctaattt acagtctact acgaagccag gtaccaaacg tttgtggatc gcgccgagat
     8041 cgcgaatcgc gtcccagacg aattctttac tccgctcgat tgcgatttct ttctggatag
     8101 tcgccataat ctcttcactc agcggctaat tcgttccgct cggttcaact caactgccgg
     8161 tctccggcac tgtcatcact tcgagcagat ggccgttagg gtcttcgaag taaaagccgc
     8221 gcccgccgcg acgagtgtag atcttgccat cggtatgatt gtagggcccg ctgccgtacg
     8281 gcagtccttt ggacttcacc cgctgatata tcgcgtcgaa ctccgcatcg ctgatgtgga
     8341 aggcatagtg atgactctga ggttccgtct cgtcggcaaa atcaaaggtg agattgtcgt
     8401 tgacctgcac ttgcgcaaaa tatccgtcgc cgggcttgac gttcaatccg aaaatctcgg
     8461 caaagaattg tgccgaggcg agtttatcct ttgccgggac gatggtgtga tcaagaacga
     8521 ttggcatacg gttctcctat ataagttaat tatcggatga tgctcttcca accgtcacgc
     8581 acctcagact cgcgttaatc gaagtcagaa cggttgaaac agctcgatcg gatttcccga
     8641 aggatcctcc acgagaattt gtttgcctcc gttgccggtg acgatctcgt tccgaaatcg
     8701 cgctccggct tccctcagtt tctctacagt ggcttcgaga tcatcgactt caatctgtat
     8761 tcggttccaa ccaccggcgc tgggaagctc gcggtcgggc attgcttgtc cggctcctcc
     8821 cgcgcccggt cgattaagca gcaactgcaa atctcctcgc gacaagctcg cgaaacctgg
     8881 cgcgggatgc atctcgagct tgaaaccaag catgctggtg tagaaaggaa ttgccgcgtc
     8941 gacatcgtcg acgatgtatc ggacactaac tttactcatg gttttctccc tgaactgttg
     9001 cgtgcttgaa actcggcgca aaaacaatca tgtgcggatt cgggcacgcc tggcggcaac
     9061 acaatcacga acattgcttg ttcccgagat accgcagagg ctaggcgccc agcttcggcg
     9121 cggccgggcg cgcgatcagg cgatcgacat aggcctcgag cttgggtcga tcgggcttgc
     9181 caccgagcat ccgcctccac atgaacatgg aaccgatcat cacgtcggcg gcggtaaacc
     9241 actcgccaaa caggtaagga ccgctgccga gttcgccctc gataagattg cgcacggtct
     9301 cgaaatccga ccagccgcgc tgcggcgtgg tctggccgcc caacatgacg tcacccaagg
     9361 cgggttcgag ctgcgctgtc gaataaacca tcaacgaaag atatcgcccg cgctcggggg
     9421 cgccgatctt cggagcaagt ttaacttccc caaacttgtc gcccaggtac aaacagatcg
     9481 ccgctgtttc gaagaccttc gactcaccgt cgtgcagcgc cggcaacttc ccggccgggt
     9541 tgatcttgag aaactccggt gtcttgtgct cgcccttctc aaattcgatg ggcacgatct
     9601 cgtatttagc gccgcactca tcgagcatcc acttcgcgat aacggcgcgg ctcttgggat
     9661 tgtagtacag tttcatgatt tcacctcctc gtccgtttag ttggcgcttt tcgatggttg
     9721 atctcgatga ctatcttaaa tggttgttgc gtttcatcaa tacgacgaat gagccttggc
     9781 ttgcaggaca ggcgaagttt gtctcgcgtt ggtcgggtga gctttaagcc cgaaatagga
     9841 ccctcaaggg aagccgccga tgcggctgaa tgctcgaaca gataagtttc tccaccttgc
     9901 tgttcggctt cgtttcggag cccaagtttt cagcgaaccc tgcaactgcg atcagcgcct
     9961 cggccacagg attgtaatcc tgcatgaagc tcgcgatcga gcggatgagt ttgtactgat
    10021 ttgcaaaatc tccggcacgt ttgtatcccg gcaatcatta caaagaccga gaagccattt
    10081 ctcttagttg gcttttttct cctgtggata cgccggcagg tcatcgccaa ggcgaatcca
    10141 tgagagctta ctctcggtcc aggtatgatt ttgcggctcg agttgctctg gatcgtccat
    10201 gcttcccaga gttaaatcga ccgactccgg cagatctaga cgacggtagg tgagcgcggt
    10261 gccgcaccgg ccgcaaaaag tgcgcactac gttcttcgac gaggcataac aaacaggttg
    10321 gccttgtgtg aagttgaact tatccgaatc gaatcccgcc cacgttacta atggcgcgcc
    10381 actggcgcgc cggcacgtcc ggcagtggca atgggttatc ccgtagccgt tccccgacgc
    10441 gcgatatcgg atcgcgccgc agaggcagcc cccttctatc gctctcagcg attgcatcat
    10501 ggtacctccc aaaataatcc ctggcgaacg tgtaagcctg gagacaaaag tttttcggcg
    10561 cgcaatcgaa tctgatcttc cttcgggctc tctcatgcga tcgcacccta cgattgtcgt
    10621 cgcaggctga aagcgaagct ttcggccaag aaaatcccct tcgaaatctc gatggcggct
    10681 ctcgctgatt ctcacgcaag ctttgcgatc gactagaaga tacccgcgtt cacaagaaag
    10741 aatatatctg ccctgccagt ggttcaagat gattactctc tcatcgaagt tcgaatggca
    10801 tctgccggcg cagcaacaat caagatcgca cccgtcgaat tgcgcaactg cgccgcaagc
    10861 tcgagacgct tcgaaaaacc gcccggaatt ttgcaggaga ctattttatg cctgaagcat
    10921 tcggccaggc cgcgaggatg cgagatctct aatccttgac ccgatagccg cgcgtttcag
    10981 cctcggagat gcactgtttt tgctgattgc gaaccgagat agcgccgaag aagcccgaac
    11041 cgctggtttc gcaggtaagc aggtcgcctt gactgttcac cagaatggtt cgctcggttg
    11101 cgcaaccggc caaactaaag gaaagggcaa gaagaactat cattcgcata aactgtatcg
    11161 aatatatcgc cttcaggcgt cggttagcaa agggctgagt tgttcgaacc aaacgccgat
    11221 gccgccgtga taaacgtagt cggcaatatc atccaacgga gtctcatcgc gattaacgat
    11281 cgccagaagg gcgccgttgc gcttggctat aaccggaaaa gaggcggcgg gttggacctg
    11341 gagcgaggag ccgacgacga tgaaaacatc agccgcctcg gtccaaattt gcgcgcggcg
    11401 catgggttcc gcgggcatct gttgaccgaa agatatattt gctgatttga gaaagccgcc
    11461 gcaggcatcg caggtagggg ccgagaactc ttctgccaag ccttcgtaaa cgtcggcggg
    11521 ttcaaagcgc ttgccgcact tcaggcaggt gaccaggcga tcggttccgt ggagttcaac
    11581 cagcttttcg tcgcaaacgc cagcgagctt gtgcagcccg tcgatgtttt gcgtgatcag
    11641 gccaagcagt tggccgcgct gctcgaagtc tcggatcgca tagtggccga tattcggctt
    11701 gacgctcttg aagagttcgt aagtcgcttt cttcaagcgc caatgctgaa tgcgcgccgc
    11761 ttcgctggct aggaaatcct gaaaataaac cggttggtat tgggtccaga ccccacccgg
    11821 gctgcgaaaa tcaggaactc cggactcagt gctgatcccc gcgccagtaa aaaaaacaat
    11881 gtcacgatag cttcgtattt gcgcggcaaa ttgttccagc tgcatcaagc tacctcatca
    11941 aaattactgc tgtggcgcac cgcaaccagc ttataagttg aagtgactga actcaatgtt
    12001 ggagatcagt accagcacgc gaggcggcac atccaggttc gtagctgcca atacccacca
    12061 gcccgatgcg cctgtcatcc ggcatcgcgc acgggtgaat ttcccgttgg gcgccaccgg
    12121 cgcattcgag cgtcgccctt ttaagttatt acttcgatga gacaatccat caaacagtat
    12181 gatcgagctt gatccccttc ccgctaaaca gtaacctcgt gcaacaaatc ccctgaagat
    12241 aggcggtcat tctatgattt catccgcttt ccagcttgat ctcgcgccga cagggaaact
    12301 gcgtgccggc atcaactatg gaaatcccgt gctggcgagg agagacccga ccagcggaca
    12361 gctcggcggc atagcggtcg agctcgctcg cgaactcggc agacggactg acttgccggt
    12421 cgagctggtt ggttttgaat cggccggcaa aatggttgaa ggagtacaag ccggtgcctg
    12481 ggatgtcgca ttccttgctg tcgattctgg gcgtgaacac gaaatcagtt tcaccgcgcc
    12541 gtacatagaa attgaaggca cctatcttgt gccgccaggg tcggcgctta gcgccatcgc
    12601 ggatgttgat cgtgagaatg ttcgcgttgg tgtgtcaagt aagagcgcgt acgatctgtt
    12661 tttgagccgg aatctaaaac aggctcaact ggtccgggcg tccacccccg aggctgcgtt
    12721 cgagttgatc gtcgctggca aggtggacgt gctcggaggg gtcagacagc acctcgtggc
    12781 aaacagcgtg aagcttcccg gagcgcgtgt gctcgacggg cgcttcatgg cgattcagca
    12841 ggcactcggc attctaaaag ggcgccaggc gggcttaagc tacctccgtg aatttatcga
    12901 agacgtaaag gcttccgggt ttgttgccca ggcgctcgag aaagctggag ttcagggcgt
    12961 ctcgatcgcg ccgaggatga tcagtaagta actcggtgtt tagggatgct caattcaacg
    13021 atcgaatcga gcggcagcag cacctagatc atacgtcgtc gtcagattga cgccggttga
    13081 tcgagctcga agccgagatc ttcgatcatg cggtgcgcgt cggacgcttg ttgacctggg
    13141 gtcgtcagat aaccgttgac gaagattgaa ttggccgggt agaggctcag cggctgaagc
    13201 gagcgcaggt tgacctctcg tcctccagcc acgcgaattt ctttgcttgg attgacgaat
    13261 cggaacaggc aaagcgtctt caaacatttt tgtggagtca gctgtctctt ggcttcgaaa
    13321 ggagtcttgg gaatggaatg cagaaagttc accgggatgg attcgatatc catagctcga
    13381 agggtaaccg caagattgat gatgtcgtcg tcgctttcac ccatgccgat gatgccgccg
    13441 gagcacgtgc cgacacctgc cgacttgacg ttttccacgg tcgccacccg gtcgtcgtag
    13501 gtgtgagtcg tgacgatttc cggatggtgt tcgcggctgg tattgaggtt gtgattgatt
    13561 cgtccgacgc cgacctcttt gaggctgcga atcttgtcct cgctcatcaa acccagtgag
    13621 cagcagatgt tgatgccgac ctttgcgcga atttctttta ccgcttcggc gatctgttcg
    13681 atctctttag gggtcggccc gcggccgctg ttgacgatgc agtagcgcac tgcgccggct
    13741 tctttggcgc gcttggcgcc ttcgaccagc tgttctttgg aaaggaacgg atagcgctcg
    13801 atgggagcct cagaaacgga cgactgggag cagtagtggc agtcctccgg gcatagaccg
    13861 cttttggcat tttgtagcat gtggatctgc acccgcttgc cgaagtaatg atgacgcacc
    13921 ttgaaggcgg cgctgacaag ctctggaatc tgttcatcgg aagcgttgag cactgccttc
    13981 atctcatccc gttccaagag ctctccttga agcgatttat cagcgagttt gccaaaatcc
    14041 attaacgcct ccggtgtggc aatttctagc acgcgtttga accccttgca aatcttgggt
    14101 ttttatccgg gacgaaaaaa ttaaactccc agatcttggg atctaagtgt gtgcctggct
    14161 gtgatcactg gcaagcccga gggctttact cggctttctc gg
//
LOCUS       DASUVS010000072         5500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_243926, whole genome shotgun sequence.
ACCESSION   DASUVS010000072 DASUVS010000000
VERSION     DASUVS010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243926"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..1217)
                     /locus_tag="VF182_04295"
     CDS             complement(249..1217)
                     /locus_tag="VF182_04295"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6436326.1"
                     /translation="MAKGILVTGGSGLVGAYAVGMLLERGERPVVFDLAINERLLAAV
                     GVDGKQVTLVRGDMADLPALISTLREYDCDRIIHLAAFLGEEVQRRPYSGVRLNFMGT
                     VNVFEAARLEKVSRIIFPSSGTVYLGSIGEGLAKIDESIPVNPPSIYAATKASCEFMG
                     RAYAKRYGFEFICLRYVGGLYGPSPVALKATREVAIQQMIRAAIKGEPAKIKWPYGAA
                     EILYGKDAAKGTVLAVLHEKFKDNLFHIGNGQMVGGEEIIAAIRQNFPESKIDLIKGD
                     NPMPYPESRLASDFSRSKEQLGYQPDYPIDKAVADYAATLTQLEHL"
     gene            1446..2435
                     /locus_tag="VF182_04300"
     CDS             1446..2435
                     /locus_tag="VF182_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436327.1"
                     /translation="MATKVALALAGLISFLIATSPANAQERLRLAWAGFSPTNSPIWV
                     MEDRKLLQKMGVEPEIIAINASPTVLQALLANEIDAASISVTTLTSSRLQGADTVMIV
                     GVVPTFVDHIVSLSSITKVEQLKGKTGAVNRLGSTSDLGLRLALRKLGVDPEKDVKII
                     PAGGNPERFAALSKGLVQFTIMPEPFLTQAEKLGFRNLFNVTDLKIPFWWNGILSREA
                     IVKKNRPLLLKLTRAMVEAIHIIKTEKEYAKGLFKKNLGVADPEGLERAYQDYSNVFP
                     EIPYPNPEGVKTMLDDLAPRNPKAAAADPKTFVDMSLVKEVETSGLIKQLYKK"
     gene            2453..3286
                     /locus_tag="VF182_04305"
     CDS             2453..3286
                     /locus_tag="VF182_04305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6436328.1"
                     /translation="MAAEPEVLPDEHPLMRQGMGRLRGKIAVITGANSGIGRATARLF
                     GREGAKVLCCDIQETITPRIDELITREGGEAVFNKIDVTRQEDCERLLSAAIEAFGDL
                     DILYNNAGAGIRKKLHEHTDDEWNFVLNTNLNAMFRGARAVLPHFIKKGSGNIVTTAS
                     TFGLLASAEYPGYCATKAAIVNLTREMALDYGPLGIRVNCVCPGAIETPRFRGFPPKP
                     TLSGITEEQRKAMGSSNKALLRMGRPEEIAYGVLFLVSDEASFVTGHALVIDGGQTID
                     A"
     gene            complement(3500..4591)
                     /locus_tag="VF182_04310"
     CDS             complement(3500..4591)
                     /locus_tag="VF182_04310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018939322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6436329.1"
                     /translation="MTNDPPTTPFSQAKADDFANRLLSMLNHSSLCVMISIGHRTGLF
                     DTMSKLHPTSSGEIASKAGLNERYVREWLGAMVTGGVVELDPNTYKFSLPSEHAAALT
                     RAAGANNMSVFAQYIGVMGSVEDEIVECFYKGGGVPYSGFPRFHEVMAEDSGQSVLSS
                     LESHILPLVPGLSDQLANGIQMLDAGCGRGLIMIRLAELFPKSRFTGIDLSSEAVMFA
                     WKTAAEKKLRNVEFIVADLSNFDQTAEVEAFDLITTFDAVHDQKFPLNVLKGVYRALR
                     PNGVYLMQDIKGSSHVHKNLDHPIGPFLYTVSCMHCMTVSLAQGGEGVGAMWGEEMTR
                     DYLQKAGFRSVEKRELAHDIQNNWYVVRK"
     gene            complement(4763..>5500)
                     /locus_tag="VF182_04315"
     CDS             complement(4763..>5500)
                     /locus_tag="VF182_04315"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX6436330.1"
                     /translation="GNFPVVLVAPHGGREAIPGIGIRQGANVLQFKTRRDHHTAELAA
                     AIALKLGELSGAKPFLVVARFDRKYVDANRPSSGAYEALEAQPYYAAFHRAVDEACKQ
                     IRKVWGRGLLLDIHGQAAEGNVIFRGTRHGKSVADLIHRFGVQALSGRRSILGQLALS
                     GYNILPDPRVNDRESRYSGGHTVRTYGSDRANGIDAMQLEFGTRLRASDNLDRTAADV
                     AAAITVFAKEYLPITSKSIEPAIGTQP"
BASE COUNT         1323 a         1472 c         1445 g         1260 t
ORIGIN      
        1 tttggcgaaa ctcatgttat tttctgcagc ctccttataa ttttaagcct tagatagttc
       61 gtagcttcag tgattagacg atatcatgga atctgcgctt cagcttgtgc ttggcgagct
      121 ctgcctgatt gttaagcggc cgcctttagg agtactttga attcaaatct ccctccctgg
      181 tccacgtgta ttaaccaagg ctcctcgtta aaaccggatg tggatcctcg gctgatgttg
      241 atccacggct ataggtgctc gagctgtgtg agggttgcgg cgtaatcagc aacggcttta
      301 tcgatcggat agtcgggttg atatccgagc tgttcttttg agcgcgagaa atcactcgca
      361 agacgagact cggggtaagg catcggattg tcgcctttga tcaagtcgat cttcgattcg
      421 ggaaaattct gtcgaatggc agcaatgatc tcctcaccgc cgaccatctg gccattacca
      481 atatgaaaca gattatcctt gaatttttcg tgcaatacgg cgaggacggt tcctttcgcg
      541 gcatccttgc cgtacaagat ttcggcagcg ccataaggcc atttgatttt tgccggttca
      601 cctttgatag cggcgcggat catttgctgg atcgcgactt cccgggtcgc tttgagcgcc
      661 actggcgacg gaccgtagag gccgccgaca taacggaggc aaatgaactc aaaaccgtaa
      721 cgtttggcat aagcccggcc catgaactcg caactggctt tggtagcggc atagatcgaa
      781 ggcgggttta ccggaatgga ttcatctatt ttcgctaaac cctcgccgat agatcctagg
      841 tagactgttc ccgaacttgg aaaaatgatc cgggaaactt tttccaggcg agccgcttcg
      901 aagacgttga ccgttcccat aaaattcaga cgcacaccgc tatacggacg gcgctgaact
      961 tcctcgccca gaaatgctgc aagatgaata atccgatcgc agtcatactc gcgcagcgtt
     1021 gatatcaggg ccggcaggtc cgccatgtcg ccgcgaacca aagtgacttg ttttccgtcg
     1081 acaccgaccg ccgcaagcag ccgctcgttg attgccaaat cgaaaacaac cggccgttcg
     1141 cctcgttcca atagcatgcc cacggcatag gcgccgacga gccccgagcc gccggtgaca
     1201 agaattccct tggccatctt ggctcctcca ggaactgcct ctagtatcgt aaaccgtcta
     1261 ttgagtcaac gtagtcattt ttatctattt caacgccctt tgtacgagtt ttaccgattc
     1321 aaagttaccg aaggctagcc ggtgacgcca ttgctaagac tttggtctga cgaagatgag
     1381 tgtttgcttg atttcatgaa gagcaaaatg gtagttatgc ggcggtttcc aaggaggatt
     1441 tgttgatggc aactaaagtc gctttggcat tagccggatt gatctcattc ctgatcgcga
     1501 cttcccccgc gaatgcacag gaaaggctca ggcttgcatg ggccggtttc agcccgacca
     1561 atagtccgat ctgggtcatg gaggatagaa agcttctaca aaagatgggt gttgaaccgg
     1621 aaatcatcgc aatcaacgcc agccccacgg tactccaagc gcttctggca aatgagatcg
     1681 atgcagccag catctctgtt accacgctga ccagctcccg ccttcagggt gccgacaccg
     1741 taatgattgt cggggtagtg cccacctttg tcgaccacat cgtttcactc tccagcataa
     1801 ccaaggtaga acaactcaag ggaaaaaccg gcgcggtcaa tcgtttagga agcacatctg
     1861 acctaggtct tcgtcttgcc ttgaggaaac ttggagtgga tccggaaaaa gacgtcaaga
     1921 ttatccctgc aggaggcaat cccgagcgat tcgcggcgct cagcaaagga ctggtgcaat
     1981 ttactattat gccggagcct ttcttgaccc aggcagagaa gctcggattc cgcaacctct
     2041 ttaatgtgac cgatctcaaa attcccttct ggtggaatgg cattctcagc cgagaagcga
     2101 tcgttaagaa aaatcggccg cttctgctca agctcacccg ggccatggta gaggccatcc
     2161 acattatcaa gaccgagaag gaatatgcca aaggtctctt caaaaagaat ttaggcgtcg
     2221 ccgatcctga aggccttgaa cgtgcttacc aggactattc aaatgtattt ccggagatcc
     2281 cctacccgaa tcccgagggc gtgaagacca tgctcgacga ccttgcgccg cgcaacccca
     2341 aggccgccgc ggcagacccc aagacattcg tcgatatgag tctggtcaaa gaggtggaga
     2401 cctctggttt aatcaagcag ctttataaga aataaaccac ggaggttgaa tcatggcagc
     2461 agaaccagaa gtactcccag acgaacatcc actgatgcgc caaggaatgg gccgcctacg
     2521 tggcaagatt gcggtgatca ccggagcaaa tagtggaatc ggcagggcca ccgctcggct
     2581 cttcgggcgc gaaggcgcta aagtcctctg ctgcgacatt caggaaacga tcactccgcg
     2641 aatcgacgag ctgatcacac gggaaggcgg tgaagcggtt tttaacaaga tcgatgtgac
     2701 caggcaggag gactgcgagc gcctgctcag cgccgccatc gaagcctttg gcgacttgga
     2761 tattctttat aacaacgccg gcgccggcat tcgcaaaaaa cttcatgagc ataccgatga
     2821 cgagtggaac ttcgttctca ataccaactt gaacgcaatg tttcgcggcg cccgcgctgt
     2881 gctgccgcat ttcatcaaaa aaggcagcgg taacatcgtc accaccgctt ccaccttcgg
     2941 ccttttggct tcagcggagt atccgggtta ctgcgccact aaggcggcca tcgttaatct
     3001 cacgcgtgag atggcattgg actatggtcc gctcgggata cgagtgaact gtgtctgccc
     3061 cggcgccatc gagactccgc gcttccgggg atttccgccc aaaccgacct tgagcggtat
     3121 caccgaggaa cagcgcaagg cgatgggctc cagcaataag gccttgctgc gcatgggccg
     3181 tccggaagag attgcctatg gcgtactgtt cctggtctcc gatgaggctt cgtttgtaac
     3241 gggccatgcg ctggtcatcg atggcggtca aaccatcgac gcgtagagga actggggtgc
     3301 tggagtagcg gagtggcgga gttttgggtt tagaaacaat tacactcgcg tatgttgtag
     3361 tgaagaccta tcaggctgat caaaaaggtt cagatgcaag gcgcgcgatg atcgacgagc
     3421 gaaggcgtac tcggcagtac gtcgagcgac tcggacaagc gcaacgcagc agatgagcct
     3481 tttttatcag cctgccacgt tactttcgaa cgacgtacca gttgttttga atatcatgcg
     3541 ccaactcacg tttctccact gatctaaatc cggctttttg aagataatcg cgggtcatct
     3601 cttcgcccca catcgctccg acgccttcac cgccctgcgc cagggaaacc gtcatgcagt
     3661 gcatgcatga aacggtatac aaaaacgggc cgatcggatg atctagattc ttgtgaacgt
     3721 gactggaccc tttgatatcc tgcatcaggt agactccgtt gggtcgaagg gcgcgataaa
     3781 cgcctttaag aacattcaat ggaaattttt gatcgtgaac cgcgtcgaac gtggttatca
     3841 gatcgaatgc ttcgacttct gcggtttgat cgaaattact cagatcggca acgataaatt
     3901 ccacgttcct gagtttcttc tccgctgcgg tcttccaagc gaacatgacc gcttcgcttg
     3961 agagatcgat gccggtgaat ctgcttttgg gaaagagttc agctagccga atcatgatga
     4021 gtccgcgacc gcagccggca tcgagcattt gaatgccgtt tgccaactga tccgacaagc
     4081 cgggaaccag aggaaggata tgactctcta aagaagaaag aaccgattga ccgctgtcct
     4141 cggccataac ctcatggaag cgcggaaacc cggaataggg aacgccaccg cctttataaa
     4201 agcattcgac aatctcatct tcgacgctgc ccataacacc gatgtactgc gcgaacacgg
     4261 acatattatt ggcacccgct gcgcgagtga gcgctgctgc gtgttctgac ggcaaggaaa
     4321 acttgtaggt attcggatcc aactcgacaa cgccgccggt taccatggcg ccaagccatt
     4381 cgcgaacgta gcgctcgtta agaccggctt tagaagcaat ctcgccggac gaggtcggat
     4441 gaagcttgct catggtgtca aaaagcccgg tacggtgtcc gatggaaatc atcacgcata
     4501 gcgagctatg attcagcatc gacaggagac gattggcaaa atcgtcagcc tttgcttggc
     4561 taaacggagt ggttggagga tcgttagtca tagcgcacct cttctagatc agaacgtagt
     4621 taaccagcgc gtttgagcag tcccctttca ccttagcaat tagctttaag tggatcctcg
     4681 acactgtgag aattggagca cgactagcaa gacccctaaa tgccgaaacc gagttcagag
     4741 taaactcaat cgtcaactac aatcaaggct gcgtgccaat agctggttca atgctttttg
     4801 aagtaatcgg caagtattct ttagcgaaca cggtaattgc agcggcgaca tcggcggcgg
     4861 ttcgatcgag gttatcgctc gcgcgaagcc gggttccaaa ttccaactgc atagcatcga
     4921 tgccgttagc gcggtcactg ccataagttc gaacagtatg gccgccgcta taacgcgact
     4981 cgcgatcatt aactctcggg tcgggcagga tgttgtaccc gcttagagct aactggccga
     5041 ggatacttcg gcgcccgctc agtgcttgta cgccgaatct gtggatcagg tccgcgacac
     5101 ttttaccgtg cctggtgcca cggaagatca cgtttccttc ggccgcttgc ccgtggatgt
     5161 cgagcagcaa gccgcgtccc cataccttcc gtatttgctt gcaggcttcg tcaactgccc
     5221 ggtgaaaagc cgcataatag ggctgcgctt caagagcttc ataggctccg gacgaaggac
     5281 gattggcgtc gacatatttt cgatcgaacc gggcaacgac gagaaacggc ttggctccgg
     5341 ataactcgcc aagctttagc gcaatagctg cggctaattc cgcggtatga tgatcacgcc
     5401 gggttttgaa ctgcagcaca ttcgcgccct ggcgaatgcc gattcctgga atggcctcac
     5461 ggccgccgtg cggcgcgacc agaacaaccg gaaagttacc
//
LOCUS       DASUVS010000073        15522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1336928, whole genome shotgun sequence.
ACCESSION   DASUVS010000073 DASUVS010000000
VERSION     DASUVS010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 15522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15522
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336928"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1115
                     /locus_tag="VF182_04320"
     CDS             99..1115
                     /locus_tag="VF182_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436331.1"
                     /translation="MRKAVILIVASAGLALGLHSAEAQSKRKVVFGVPVTPPNVVHIP
                     PYVAKEMNFFGEQNIEVELVTFEGGTQTLRGSVAGGLDITGTSSDPAIIAAARGAGTK
                     VLGTYSHKLSQSMLVQGDIKSCKDLKGRKIGIQEVGAFNEVMSRAVLASCGLTPKDVD
                     YVPVSTKGRVAGLLSNQIDTAILHVDQAIVAKKKKPELNILVNLWEPLPKWLYAAYIA
                     PEKEIAKNRQLYVDIMTALVKANRFIYSNKAKTVEIAAKYTQQDADAIGQTYDILANA
                     GAWPVNEGLPRDMVEWTIDKQLELGSIKAAEKPTYEKLVDISIIEAAIAKSGGRLKGD
                     KRWD"
     gene            1452..2468
                     /locus_tag="VF182_04325"
     CDS             1452..2468
                     /locus_tag="VF182_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6436332.1"
                     /translation="MIRHLLCLWLNAIVLFLLLLDSPAVLAQAVNLEAARKEGKVIAY
                     GTIIPQVMEPLHRDMEKKYGVKVEYWRASATQVMERAHSEWQAGRPGFDVTFAINGAQ
                     AILKQAGVFAKYTPASAEKFPAAFKDKDGLVTAWRHTPIGVLYNTDLVPAADAPKNFD
                     DLLQPKWKGKIAIPDASRHTSTAQFLVSLRKIKGEGWIDYVKALAKQAPLLKESFAPV
                     PNDILRGEALLGFTYLQYVYQNKGPLGYVLMDKILTDTNDLGLSAKAMNPNAAKLYME
                     YLCSPEGQKLVADLGEFVLSPGIYPPVKDADKITANSIFMDNPSAEEYKKLSAEFRQI
                     FFGK"
     gene            3144..3764
                     /locus_tag="VF182_04330"
     CDS             3144..3764
                     /locus_tag="VF182_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014516571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA
                     nucleotide-binding domain protein"
                     /protein_id="HEX6436333.1"
                     /translation="MNRYPEANVNSNCTSTEQLLLQIARLRDVLGQIQKFIQDRHLKS
                     CIFPGLRGGPEVASAMRELSTRRIGALIALERTIGLGQYVSSGTLLNAELSTSLLLSL
                     FYPGNPLHDGGVIIRGASIVAAGCVLPLSADSAHFKLKGKGMRHRAGLGLTQVSDAIV
                     FIVSEETGTVSMATAGQMIEIDMGDGSEAMTTWSDPVHKGEHISLH"
     gene            complement(3797..6820)
                     /locus_tag="VF182_04335"
     CDS             complement(3797..6820)
                     /locus_tag="VF182_04335"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6436334.1"
                     /translation="MASTETDELIREHSILGDVEFGGGKGIRQAAHVAYLWETHNEFA
                     DGVRFLEVGLHGADHCVIFGHEQATRAVCKILEDHGFDVQALLAERRITILEENSSRE
                     KIVQAIGAELQSPAARNAPSIRLLLNTGWGKPGWPSDYDLLAFEAKLTALTKQFPCLV
                     LCMYEVAALPGLIVRHGAYETHPLIMERGGSLRENPFHIPTPFFLERLDTVAGVISER
                     RHAEAAAHSSEDRFRSLFANSVDAVLIANPDGSIEAANPGACRLFGRTEAEICQIGTI
                     GLIDPTDSRLTLLLKEREQTGRFTGELIFRRRDGSEFLGEVSSVFYRDNSEAIKASII
                     IRDINERKRLEATLRDITAGTSRSTGDEFFRSLVKNLAYALGVRYCFVAACTDETKKW
                     VRMLAFWAGEDFSENIEFPLAGTPCENVIQGDVCSYSECLQVLFPEDRGLVSLGAQSY
                     AGVPLTNSTGTVLGHLVVIDVQPRVFSEQALSILQIFATRAAVELERKRAEQALQMSE
                     ERLRTLLDINNAIVTRLSRDELFRAISDALGRVISFDRLSLSLYDASANVLRLVTYAG
                     PYQRDDYTPIGRELDLNDSPAGLAFCSQKPVIRADLETVQRTSSEERALGHGFHSLCA
                     LPLTVRGRSIGTITIGSLTKNRYNQADAEFLMEVANQIAIAVDNMKSYEEIDALKARF
                     QSETVYLQEEIKTEHNFEEIIGQSVPMRQLLRKIEQVAPTEATVLIQGETGTGKELLA
                     RAVHSRSLRNARPLVKVNCGSIPSGLVESEMFGHEKGAFTGATQRRIGRFELAHGGTI
                     FLDEVTELPIDTQVKLLRVLQEGEFERVGSSETIHVDVRVIAATNRDLKGVVANGTFR
                     SDLFYRLNVFPLEAPPLRARQGDIPLLVNFFLSKFGKKLGRVIRGFSQKAMERLTSYN
                     WPGNVRELQNVVERAVVLAGGPIVDIDESMLYSHREMPDPAVDTLENMQRNHIVRALA
                     ESRWVIHGQHGAAEILGMNPSTLRSRMNKLGIKRP"
     gene            7014..7283
                     /locus_tag="VF182_04340"
     CDS             7014..7283
                     /locus_tag="VF182_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436335.1"
                     /translation="MLKITEHLENAKTVRLRLDGTLNALSLHEVEEICSRHQSETGKV
                     TLLDLAGVVFMNDESAKRLVELRGDTLKIINCSPFIETLLHIVET"
     gene            7337..8038
                     /locus_tag="VF182_04345"
     CDS             7337..8038
                     /locus_tag="VF182_04345"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6436336.1"
                     /translation="MSSVRIVTMPANQPEPIAIGHESHADRPTLSDRRRKSSEREAAL
                     IEGLKVGDQQAFEAIFNLYSAKLYNVAHRILGDVADTQEVIQDVFWAVYRKAKSFRGN
                     SQFSTWLYRLTINAALGRIRRSKKNKEVEYEEFLPRFQQDGHHLVSPVIDWSDTLDET
                     YAKQQIQKFLKDALDQLKPIDKSVVVLSDLEGVSDKEIAAALGITVSAVKTRLHRARL
                     FLRGKLAVQLGHSAA"
     gene            9264..10376
                     /locus_tag="VF182_04350"
     CDS             9264..10376
                     /locus_tag="VF182_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX6436337.1"
                     /translation="MKAIAVMPHIKELAIINQPEPKILSSTDVKLRMLEAGVCGTDKE
                     ICSFEYGTPPAGSEQLVIGHESLGEVVEVGPHVTRVSVGDLVVPMVRRPCPHDYCVAC
                     REDRQDFCFTGDFQERGIKERHGFMAQFVVDDEKYMNVVPKDMRDVAVLVEPLTIAEK
                     GLTQVWQVQQRLPWSCPVAPGKAAAYCHRAVVLGAGPVGLLGAMALVNQDFDTYVYSR
                     EPTPNPKAQLLESIGAHYLSSENIAPEMLPEQVGNIDLVYEATGASSLAFEMIKYLGA
                     NGIFIFTGVPGRKGPIEVDTDLVMRNLVLKNQVVFGTVNAGRDSFENSIRDLGTFTKR
                     WPKAVNSLITGRFPMESYADLLLGRSAGIKNVIRLN"
     gene            10503..11288
                     /locus_tag="VF182_04355"
     CDS             10503..11288
                     /locus_tag="VF182_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015155608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX6436338.1"
                     /translation="MTAVLKRHWPEYLMEAACLGLFMISAFSFGAILEYPGSALHQAI
                     PYPLLRRFLMGIAMGLTAILIIYSPWGKQSGAHINPSTTLTFWRLGKVAKWDAVFYVV
                     AQFGGGVSGAILASAVLSAWVAHPSVNYVVTMPGSAGVTAAFFAEVAITFILMSVILR
                     MSNNSHLHKFTGLCAGALVAAYITLEAPISGMSMNPARTLASAVPAHYWTALWLYFTA
                     PLIGMLAAAEIHLRSRSPGIGCAKLHHDNPKRCIFCGKPPSEA"
     gene            11480..11977
                     /locus_tag="VF182_04360"
     CDS             11480..11977
                     /locus_tag="VF182_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX6436339.1"
                     /translation="MKLIMAVLGILTLLTLSSVGAAQIIEKKGLTLDGAKKVIAAAVA
                     EARSKNAPGGAIAVVDEGGNLVALERLDNTFAAGANISIGKARTAVLFKRPTKFFEDA
                     INKGRTAMTTLNDFTPLQGGIPIIADGQVIGAIGVSGAASAQQDEELAITGANALATS
                     SAKAQ"
     gene            12000..12860
                     /locus_tag="VF182_04365"
     CDS             12000..12860
                     /locus_tag="VF182_04365"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX6436340.1"
                     /translation="MKTIKTLIALWFFLLPATGLQAQLAEKKVLTLEAAEKITAAAEA
                     EAKKRAATVVIVVVDDGGYPIVLKRLNDTQVASVDVGIGKARTAAIFRRSSKDFEDQI
                     KNGRLASLVLPGATPLQGGVPLISDGKVVGAIGVSGNTPQEDEDIAKAGAASFEASDK
                     TASAPVTYLPAKQVSHAFVKGMPLVRGNNYKVDASHRDGAGVFEVHTRDTDIIYMLEG
                     TATLITGGTLVDARSIEAEEIRGLESNGGEARTINKGDVVVIPNGTAHWFKEVKGPID
                     YYVAKVRAAD"
     gene            12875..14533
                     /locus_tag="VF182_04370"
     CDS             12875..14533
                     /locus_tag="VF182_04370"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX6436341.1"
                     /translation="MRRIFVLTGIALLLTLPAKLVAGNSPEESPATHKADAVIDLATK
                     QGVEMVRGQWRYSDTKIIEVDFKAAGLDGQPGDMPIKAYDFTPHAGGADFDDASWEVL
                     DPTTLDKRRSAGRLAFNWYRIKISVPERIGNFDPAGSTVVFATSVDDYAEIWVDGELP
                     RAAGQSGGSVIKGWNAENRLVVGRDVKPGQKIQLAIFGINGPVSNPPTNYIYMRYAKL
                     EFHKIAAGPIAVTPQEVNVEVVRLDPAIDAIVPPNPKIFKLAEGFQFTEGPIWTPSGD
                     LLFSDPNANKIYKYAANETLSLFKNNSGYSGKDVADYFQPGSNGLTLDPEGRLTINQH
                     GNRRVVRLEKDGKLTVLADRFDGKRLNSPNDLVYRSDGTLFISDPPFGLPKFYDDPRK
                     ELPYSGVFALYKGQLKLVSTDLKGPNGIALSLDEKYLYVGNWDPEKKIVMRYVVNADA
                     TLSDGEVFFDMTSAPGQDAIDGIKVDQQGNLYVSGPGGLWILSAAGKHLGTIKAPKHP
                     HNLAWGGEDGKTLYLTAQSALYRMPLNISGIRPADGMSKLAAQR"
     gene            14664..15236
                     /locus_tag="VF182_04375"
     CDS             14664..15236
                     /locus_tag="VF182_04375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016869520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX6436342.1"
                     /translation="MTESRPLNIPVILGTTRKGRMSEHAARFMAGQIEKRNGIKTELI
                     DLSQLPMPIDDAGEAIKDAAFSEKMTMADALVIVTPEYNHSFPGLLKHILDSCLKEYI
                     HKAAGLVGVSAGPFGGVRAIQDFLPVIRELGLVNIFWDVNFGNVSKVFDDSGKLLDAA
                     FISRADKFLNELVWMARTLRYGRDDIALDN"
     gene            15249..15437
                     /locus_tag="VF182_04380"
     CDS             15249..15437
                     /locus_tag="VF182_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436343.1"
                     /translation="MAKQKSTMICPNCGNQMNHHADKLVDPVKPEDLRHRDTALGGVI
                     EEIHGCPGCGAAASRRAV"
BASE COUNT         3730 a         4138 c         4210 g         3444 t
ORIGIN      
        1 gaatgatatt catgacaatc cgtttcattc ttgaggatga aagctcgaag cgaaggagcc
       61 agttggcatc atttcggacg agtccaaaag gaggctttat gagaaaagct gttattctga
      121 tagtggcatc ggccggcctg gcattgggac ttcattccgc cgaagcccag agcaagcgca
      181 aggttgtttt cggcgtgccg gttaccccgc ccaatgtggt gcacatcccg ccatatgtcg
      241 ccaaggaaat gaatttcttc ggcgagcaaa acatcgaggt cgaactggta acgtttgaag
      301 gcgggacgca aaccttaaga ggaagcgtgg ccggaggcct cgatatcacg ggtaccagct
      361 ccgatccggc gatcatcgcc gctgccagag gcgccggaac caaagtcctg ggcacctatt
      421 cacataagct ctcgcaatcg atgctggtgc agggtgacat caagagctgc aaagatttaa
      481 aggggcgaaa gatcggtatc caggaagtcg gcgcgttcaa cgaggtgatg agccgagccg
      541 ttctggcaag ttgcggactc acacccaagg acgtcgacta tgtgccggtc agcaccaaag
      601 gacgtgtcgc cggcctattg agcaaccaaa tcgacacggc aatccttcat gtcgatcagg
      661 caattgttgc caaaaagaaa aaaccggagc tcaacatcct ggtcaatctc tgggaaccgc
      721 tgcctaaatg gctttacgcc gcttatattg cgccggagaa agagatcgcc aaaaaccgcc
      781 agctttacgt cgacatcatg accgcattgg tcaaggcgaa ccgctttatc tacagcaaca
      841 aagccaagac ggtggagatc gcggcaaaat acacgcagca ggatgccgat gccatcggtc
      901 agacctacga tattctggcc aatgcgggag cctggccggt caacgaaggc ttgcctagag
      961 atatggtcga gtggaccatc gacaaacaac tggagctggg aagcatcaaa gcggcggaga
     1021 aaccgactta tgaaaaactc gtcgatattt cgatcatcga agctgccatc gccaagtcgg
     1081 gcggacgtct gaagggggat aagcgctggg attagcgcgc aaactgttta ccacgggacg
     1141 cgcgcaaaga ctcccgctgc gatgaaagca ttctgattat ttttgaattc cgcgattgcg
     1201 taagcatccg aagtttttga atcgtcgccg cggccagtat gcttcttcac ggcgtctgag
     1261 tgactaacag ttgggcggga atgtgggatt gatgatttct agatcccagg cagtacagcg
     1321 gagctagcct ttcagtcatt aaagtgaact tgggttcgcc gacgatgacg gcgagcaaac
     1381 agtcattgta ttcgggtaat ccgctatacg atatcttgcc gcaccctaag gaccgcaatg
     1441 gaggcgccga tatgatcagg catcttctct gcctttggct aaatgctatt gtgcttttcc
     1501 tgctgctgtt ggattcgccg gctgttctcg cgcaggcagt caacctggaa gcggccagga
     1561 aagaaggcaa ggtgatcgcc tatgggacta tcatcccgca agtgatggaa ccgctgcatc
     1621 gtgatatgga aaaaaaatat ggcgtcaagg tagagtattg gcgcgcgtcg gcgactcagg
     1681 tgatggagcg cgcgcactct gagtggcagg caggccgccc gggctttgac gttacttttg
     1741 ccatcaacgg cgctcaggcg atactcaagc aggcaggcgt cttcgccaag tacacgcctg
     1801 cgtcggctga aaagtttccc gccgccttca aagacaaaga cggcttggtc accgcctggc
     1861 gtcacacgcc gatcggtgtg ctttacaaca ccgatttggt tccggcagcg gacgcgccga
     1921 agaatttcga tgatcttttg cagccgaaat ggaaggggaa gatcgcgatt cccgacgcat
     1981 cgcgccatac cagcacggcg caatttctcg tgagcctgag aaagatcaag ggtgaaggct
     2041 ggatcgatta tgtcaaagcc ttagccaaac aggcgccgct gctcaaagaa tcatttgccc
     2101 cggtgcccaa cgacatcctg cgcggcgagg cgctgctcgg atttacctat cttcaatacg
     2161 tctatcagaa caaaggtccg ttgggttacg tgttgatgga caagatcctc accgacacca
     2221 acgatctcgg gttgagcgcc aaagcgatga atcccaacgc cgccaaactc tacatggagt
     2281 atctctgctc gccggaaggg cagaagttgg tggccgatct cggcgagttc gtgttgtcgc
     2341 ccgggatcta tccgccggtg aaggacgccg acaaaatcac cgcgaacagc atcttcatgg
     2401 ataatccgtc ggcggaggaa tacaaaaagc tcagcgccga attccggcag atctttttcg
     2461 ggaagtagag aaaggataac tgcggattcc gctaagactt agcatcgatt tgatgcaacc
     2521 tagatctttc tttgatcggg cacaatattt cttaatcgaa gaaatggcag ctccacgagt
     2581 ttaactgaac gtataatcct ggattttgca tttcattacc gtcacgttta taaatttcac
     2641 ccgaaaatgc attgagcagg cgccaggttt ttctccaagt tccgccccgc caacctgaac
     2701 ccggccttga acgacgttgt cactgaagtt aatgacgata agggagcgat cgttttcctt
     2761 aatccacctc caactcagca ggggatcttt actgtcgaaa atctttcgcg tgctccggcg
     2821 aaactttcga gaaatttgct attcactcgc agatcaaagt gtctgcggca acgggtttaa
     2881 tcgaagccgt tgtaactttg ccaacagcgc cagtttcgcg catgagtgat ttaacgcaaa
     2941 ttgccgtgat gaagaccgga gtagctatga gaatggcgcc cggcgtttag ttgatgcttt
     3001 atggtgaatc aggttgcctg ctccgctgaa ctcaaaatat aaaaccacat ctttttgccg
     3061 gcgaggcgca tctaaccggc aagagcgact tataaccgat cggataagat cgaaggtgtc
     3121 atcattcttc caagaggcag agtatgaatc gttatcctga agcaaacgtc aattccaatt
     3181 gcacatcgac ggagcagctt cttctgcaaa ttgctcggtt gcgtgatgta ttgggtcaga
     3241 tacaaaagtt tatccaggac aggcatttaa agtcctgcat ctttcccggt ctccgtggcg
     3301 gccccgaagt cgcaagcgcg atgcgcgagt tgagtacgag aaggatcggg gcgctgattg
     3361 ccttggaacg gacaatcggt cttggccaat atgtgagcag cggcaccctt cttaatgcgg
     3421 agctgagcac gtcgttactg ctgagcctat tttatccagg aaatccgctt cacgatggag
     3481 gggttatcat aagaggcgcc tcgattgtag cagcggggtg tgtattgcct ctatcggctg
     3541 attccgcgca tttcaaactg aagggtaagg gcatgcgaca tcgagcagga ttgggactta
     3601 ctcaggtgag cgatgcgatc gtgttcattg tttccgaaga gaccggaacg gtttcaatgg
     3661 ccacggcagg ccaaatgatc gagatcgata tgggagacgg cagcgaagca atgacgacat
     3721 ggtcggatcc tgttcataag ggcgaacata tctcactgca ttaaagaatc gctataaggg
     3781 ttgcgctgat caagctctac ggccttttaa tgccaagttt gttcatgcgc gatcgtaagg
     3841 tgctcggatt catgcccagg atttcggccg cgccatgttg gccgtggata acccagcgac
     3901 tttcggccaa ggcgcgaacg atatggttgc gctgcatgtt ttcgagagtg tccacggcgg
     3961 gatctggcat ttccctgtgt gagtaaagca tcgattcgtc gatgtcgaca atcggaccgc
     4021 cggcgaggac cacggcccgt tcgacgacat tttgcaattc ccggacgttt cccggccagt
     4081 tgtagctcgt cagtctctcc atcgcctttt gtgaaaagcc acgaattact ctgccgagct
     4141 tcttgccaaa tttactcagg aaaaagttca ccaataaggg gatgtctccc tgccgagctc
     4201 tcagtggcgg cgcctccaga ggaaagacat tcaagcggta gaatagatcc gaacggaacg
     4261 tgccgtttgc tacaacaccc ttcagatcgc ggttggtagc agcaatcacc cgaacatcga
     4321 catggatcgt ttcgctactg ccgacccgct cgaattctcc ttcctgtaat acgcgaagta
     4381 attttacctg agtatcgatg ggtagttcgg tgacctcatc gaggaagatg gtgccgccgt
     4441 gggccaattc gaatctgccg atgcggcgct gcgttgctcc ggtaaatgcg cctttctcat
     4501 gtccaaacat ttcgctttca accaggccgg aagggatcga accgcagttg accttgacca
     4561 gcggccgggc gttgcgcagg ctccgactat gcacggcccg cgccaagagc tctttaccgg
     4621 tgccggtctc gccttgaatc aatacggtcg cttcggtggg cgctacttgt tctatcttgc
     4681 gtagcagttg acgcatgggc acgctttgcc cgatgatctc ttcgaaattg tgctctgtct
     4741 tgatttcttc ctggagatat acggtttcag attggaatct cgctttcagt gcgtcgatct
     4801 cctcgtagga tttcatattg tccacggcga tcgcgatctg attggcaact tccattagaa
     4861 attccgcatc cgcttggtta tagcggttct ttgtcagact acctatcgtg atcgttccga
     4921 tacttcgtcc gcgcaccgtg agcggcagcg cgcacaagga gtgaaacccg tgcccgaggg
     4981 cgcgttcttc ggacgatgtg cgctgcacgg tttccagatc agcgcgtatg acaggcttct
     5041 ggctgcaaaa tgccaatccc gccggactgt cgtttagatc gagctctcgc ccgatcggcg
     5101 tataatcgtc acgctggtag ggaccggcat aggtgaccag tcgcaggaca ttggcactgg
     5161 cgtcgtaaag cgaaagggaa agccggtcga aggaaatcac gcggccaagg gcatcggaaa
     5221 ttgcgcgaaa cagctcgtct cggctcagcc ttgtaacgat ggcattgttg atatcgagta
     5281 gagtgcgcag ccgctcttca ctcatctgaa gcgcctgttc ggcacgtttt cgctccagct
     5341 ccaccgcggc tcgggtagcg aatatttgca ggatcgataa cgcctgttcg ctgaagaccc
     5401 tgggttgaac gtcaatgacc accagatgcc ccagaaccgt tcccgttgaa ttggtcagag
     5461 gcacaccggc ataactctgc gcgccgagac tcaccaggcc tcgatcctcc ggaaatagaa
     5521 cctgcaaaca ctccgaatag gaacagacat ccccttggat gacgttttcg cacggagtgc
     5581 cggcaagcgg aaattcgatg ttctcgctaa aatcttcgcc cgcccaaaaa gccagcatac
     5641 gcacccactt cttggtttcg tccgtacacg cagcgacaaa acagtaacgc accccgagtg
     5701 cgtaagccaa gtttttcacc agcgagcgaa agaattcatc tcccgtgctg cgggacgtcc
     5761 ctgcagtgat gtcgcggagg gttgcttcaa gccgcttgcg ctcattgatg tcacggatga
     5821 tgatgctggc ctttatagct tccgagttgt ctcgataaaa aacgcttgag acttcgccga
     5881 gaaattccga gccatctctg cgcctaaaaa taagctcgcc tgtgaaccgg ccggtttgct
     5941 cgcgctcttt taagagtagc gtcaatctac tgtccgtcgg atcgatcaat ccgatagtcc
     6001 cgatttgaca gatctccgct tccgttcgcc cgaacagccg gcacgcgccg ggattggctg
     6061 cctcgatgct gccatcagga ttagcaatca gaacggcgtc gacgctgttt gcaaacagag
     6121 agcgaaagcg atcttcgctg ctgtgcgccg ctgcctccgc gtgccgccgc tcggagataa
     6181 cgccggcgac cgtatccagg cgctcgagaa aaaaaggcgt tgggatatgg aagggattct
     6241 cgcgaagaga gccgccacgc tccatgatca atggatgcgt ttcataggcg ccgtgacgga
     6301 cgatcaatcc cggcagcgcc gccacctcgt acatacaaag aaccaggcaa gggaactgtt
     6361 ttgttagagc cgtcaacttc gcttcgaaag cgagcaagtc gtaatcgctg ggccagcccg
     6421 gtttgcccca gcccgtattt agcagcaaac gaatcgaagg cgcgtttcgg gctgccgggc
     6481 tctgaagctc ggcaccgatg gcttgaacga ttttttctcg ggaagaattt tcctcgagga
     6541 tggtaatgcg ccgctcagct agaagcgcct gaacatcgaa accgtgatcc tcgagtatct
     6601 tgcaaaccgc tcgagtggct tgctcatgac cgaaaatcac gcaatgatcc gcaccatgca
     6661 agccaacctc gaggaagcga accccgtcgg caaattcgtt gtgcgtttcc cacaaatacg
     6721 cgacgtgagc cgcttggcgg attcctttgc caccgccgaa ctcaacgtct ccaagaatcg
     6781 aatgctctcg gatcagttca tctgtttcgg ttgaagccac aagaagatta ctccggctgg
     6841 atctctcgtg agttcatact tcttgtcgtg aaatatcgca accgttgcga catgtcgcga
     6901 gcgataatgc aaagatcggc cgcgttaaac tagaaaattc aggggaatta atcccttagg
     6961 aagctggcac gtgccatgct ctaagagttc tccgaatcga aaatgaagct cccatgctga
     7021 aaattacgga acatttagaa aatgctaaga cagtcaggct ccgattagat gggacgctca
     7081 acgcactgtc gttgcatgaa gttgaagaaa tttgttctcg tcatcagagc gagactggca
     7141 aagtcactct gctcgatcta gcgggcgtgg tcttcatgaa tgatgaaagc gcgaaacgat
     7201 tggtggaatt gcgaggcgac acgctaaaaa tcatcaattg ctcccctttc atcgagacgc
     7261 tcttacacat tgtagaaaca tagacggcaa aattatggaa tgttctttgc ctcgatggac
     7321 agacaggaga gatgttatgt ctagtgttcg tatcgtcaca atgccagcga atcagccaga
     7381 gccaatcgcg atcggccatg aatcgcacgc ggacagaccc acgttatcgg atcggagaag
     7441 aaaatcgagc gagcgcgaag cggcgctcat cgagggactg aaggtcggtg accagcaggc
     7501 gtttgaggcg atcttcaatc tttattccgc gaaactctac aatgttgcgc acagaatcct
     7561 cggcgatgtt gcagacaccc aagaagtcat tcaggatgtt ttttgggcgg tgtatcgcaa
     7621 agcgaaatcc ttccgtggca actcacaatt ttcgacctgg ctgtaccggt tgacgataaa
     7681 tgcggcattg ggaaggattc gccgaagcaa aaaaaacaaa gaagtagagt atgaagaatt
     7741 cctgcccagg tttcagcaag acggacatca cttggtaagc ccggtgattg attggtccga
     7801 taccctggac gaaacttatg ccaagcagca gatacagaaa tttctcaagg atgctctgga
     7861 ccaactgaaa ccgatcgata aaagtgtcgt cgtccttagt gatctggaag gtgtgtccga
     7921 caaagagatt gctgcggcct tgggaatcac tgtttcggcg gtaaagacga ggcttcatcg
     7981 agcccggtta tttttgcgtg gcaaattggc tgtgcagtta ggtcattcag cggcttgagg
     8041 gatctatggc gggaccaagc agacggcagc gaataccgag tgccaagact tgcaaacagg
     8101 tgaccagtct tatttctgat tacttgaacg acaaactggc tcccgctgtt aagcgtgatt
     8161 ttcagcaaca tctgcggctt tgtccggact gtgttagctt cctgaatacc tataagaaga
     8221 gtgtgtctgt aactcgctct atcggtcccg aagaaatccc gcccaggatt agagataata
     8281 tcgtcaattt tttacgccgc cgcgtccgga aatccggcag caaatcatga gtagcttgtc
     8341 gatttaagct aagcactccg agcgccggct ttattgcgta tcaatttagc ccgaactacg
     8401 cagttcaaaa aagaatggtt atcaaggcag cgagcttcca gtgcgaatgc catgggccag
     8461 tggcttggcg cgcctgcaat ccccagtggt gcagaggact ccctggacaa attacgagca
     8521 tgggaccaac caccctagac agcattgaaa ttgttcgctt ctgtgccttc gctcctccgg
     8581 actcgccctt gatcagtaca catcgttaat tcctattgct gagcccgggg ttagtgagca
     8641 tctcaccagg ggaaaaccgc tatagccgct gctcggatcc accgcccaga aggcacattc
     8701 cagccagctc atcggcccgt aaagtttctc gcgccggcgg cttagattct gccaaggaca
     8761 ttctttcgcc gtcattcgcc cgggatgcga tgtccttgaa acttttgttt ctgccggcgc
     8821 atctaacgtg aacccatgaa aaaaaaccgg aagcacacac tgcgctctag gtcgaaccga
     8881 tcgaatgaaa gttcacgcga gaataagcca cctcttcaaa atcccgctac agcaaaccct
     8941 acgtgtaaag atgaggtgac cctgattggg gattatttgg ctgggagttt ggccccggct
     9001 gttctcgtgg cgttcgaaga acacctcgtc ctgtgccgcg attgcgcggc ttttttgagt
     9061 acatacaaaa aaacgattga actcactaag tcgtttctaa aaatgcaatc acgcgggatc
     9121 gagttgaaac cgctgcggct tactccgaga atgcgctgat tctcgtcgct atgttttgcc
     9181 tatatctgtt tattcttacc gccgccaggt taggatgaat tggcatcgtt tccccttgag
     9241 cgccaatttt taggaggcca ctgatgaagg ctatagctgt tatgccccat ataaaggaac
     9301 tcgcgattat caatcaaccg gagccgaaga ttctctcctc caccgatgtc aagctccgca
     9361 tgctcgaggc aggagtctgt ggcacggaca aggaaatatg ttccttcgaa tacggcaccc
     9421 cgcctgccgg ttccgagcaa ctggtgatcg gccacgaatc attgggcgaa gtcgttgaag
     9481 tcggaccgca cgtcacacga gtcagcgtcg gcgatcttgt cgtgccaatg gtgcgccgcc
     9541 cgtgcccgca cgattattgc gtggcatgcc gcgaggaccg ccaggacttt tgtttcaccg
     9601 gagatttcca ggagcgcggc atcaaagaac gtcacggctt tatggcacaa ttcgtcgtcg
     9661 acgacgagaa gtatatgaat gtggtcccga aagacatgcg cgacgttgcc gttctggtgg
     9721 aaccgctgac tatcgcggaa aaggggttga cccaagtatg gcaggtgcaa caacgattgc
     9781 cttggagctg cccggtcgcg cctggaaagg ctgcggctta ttgccatcga gccgtcgttc
     9841 tgggcgccgg accggtggga ctgcttggcg ctatggcctt ggtcaaccaa gattttgaca
     9901 cctatgtata ctcacgggag ccgactccaa atccgaaggc tcaactactc gaatcgatcg
     9961 gtgcacacta tctttcctcc gagaatatcg ctcccgaaat gcttcccgaa caggtcggta
    10021 acatcgattt agtctacgaa gcgacggggg cttcgagcct ggcttttgaa atgatcaagt
    10081 atctcggcgc caacgggatc tttatcttta ccggcgtgcc cggacgaaaa ggccccattg
    10141 aagtcgatac cgatctcgtg atgcgcaatc tggttctgaa aaatcaggtc gtcttcggca
    10201 cggtcaatgc cggacgcgac tcttttgaaa attctattcg agatctcggc acttttacca
    10261 agcgatggcc taaagcagtg aactcgctga tcaccgggcg cttcccgatg gagtcgtacg
    10321 cggatcttct tctcggccgc tccgctggga tcaagaacgt gatccgcctc aattgatccc
    10381 agaacagcgg ttggcgcacg cgaatggaac accgtaagac caacgcactg tttatgggtc
    10441 aatgttttta gggatccaga atggaacatg gaacgttgaa ggttgaagtt tcctgacagc
    10501 acatgacggc tgtgttgaaa cgccattggc ccgaatatct gatggaggcc gcttgcctcg
    10561 gcctgtttat gatttcagcc ttcagcttcg gtgcgattct ggaatatccc gggtcggccc
    10621 ttcaccaggc cattccctac ccactgctgc ggcgattcct gatgggcatc gcgatgggat
    10681 tgactgctat cctcatcatc tattcgcctt ggggcaagca atccggcgct cacatcaacc
    10741 catcgactac tctgacgttc tggcgacttg gcaaggtcgc caagtgggat gcagtcttct
    10801 atgttgtcgc gcaattcggc ggcggagtaa gtggggctat tctagcttcg gccgttctat
    10861 ccgcatgggt ggctcatcca tcggtgaatt acgtcgttac catgccagga agcgctggag
    10921 tcaccgcggc ttttttcgcc gaagtcgcga ttacctttat tctaatgagc gtgattctcc
    10981 gcatgtcaaa caacagccat ctgcacaaat tcacgggctt gtgcgctgga gcattggtcg
    11041 ccgcctacat aaccctcgaa gcgccgatct cgggaatgag catgaatccc gcgcgcactt
    11101 tggcttccgc ggtgccggca cactattgga cggcgctatg gctttacttc actgctcctc
    11161 taatcggcat gctggccgcc gcggagatcc acctgcgaag ccggagtccg ggtatcggtt
    11221 gcgccaaact gcatcacgat aatcccaagc gctgtatatt ttgcggcaaa cccccttccg
    11281 aagcatagcc ttgcgatagc gtatgggtgc gtcgtttctc tctcggcggc tattctagat
    11341 taagcccccg ggctgaatcc gtaaaagcct caagtagaat tcgcctggat cgactgctgc
    11401 gcgccgcttc acgcaccttt tctcctgaga ggcgcatcta aagcagcaat caataatgcg
    11461 agataattgg aggacatcga tgaagctcat tatggctgtg ctcggtatac tgacgttact
    11521 gactctctct tcggtgggag cggcgcagat aattgagaaa aaagggctga ctctggacgg
    11581 cgcaaaaaaa gtgatcgcgg ctgctgtcgc cgaggcgaga agcaagaacg cgccgggcgg
    11641 agccattgcc gttgtcgacg aaggaggcaa cctggttgca ctcgagcggc tcgataacac
    11701 ttttgccgcc ggcgccaaca tctcaatcgg caaagcgcgc actgcggtcc tcttcaaacg
    11761 gccgacgaag ttcttcgaag acgcgatcaa caaagggcgt actgcgatga ccactcttaa
    11821 tgatttcacg ccgctgcagg gcggcattcc gatcatcgct gatgggcaag ttatcggcgc
    11881 gataggggtc agcggcgccg ccagtgcgca gcaagacgag gaactcgcca tcaccggcgc
    11941 aaacgcctta gcgacgagct cggcgaaagc gcaatagcca ccacaattca ggagacgtta
    12001 tgaaaactat caaaactcta attgcattgt ggtttttcct tctcccggcg accggacttc
    12061 aggctcaact ggcagaaaaa aaagttttga ctctcgaagc cgcagaaaaa atcaccgcgg
    12121 ccgccgaagc cgaagcaaaa aagagagctg cgactgtcgt cattgtcgtg gtggacgacg
    12181 gcggctatcc gattgtcctc aagcggctca atgatactca ggttgccagt gtcgacgtgg
    12241 gcatcggcaa agcgcgcact gcggcgattt ttcggcgctc cagtaaagac tttgaagatc
    12301 aaatcaagaa cggccgccta gcctcgctgg ttctccccgg tgctaccccg ctccagggag
    12361 gtgttccgct tatttccgac gggaaggtgg tcggcgcaat cggtgtcagc ggcaatacgc
    12421 cccaggaaga cgaagacatt gccaaggcgg gcgccgctag cttcgaagca tccgataaaa
    12481 cggcctcagc tcctgtgacc tatctgcccg ccaagcaagt gagccacgca ttcgtaaaag
    12541 gaatgccgct ggtccggggc aacaattaca aagtcgatgc cagtcaccgt gatggagcag
    12601 gcgtcttcga agttcacacc cgcgacacgg atatcattta catgctcgaa ggcacggcga
    12661 cactcatcac tggcggaacc ctcgttgatg cgagatccat cgaagctgaa gaaattcgcg
    12721 gcctggaatc caatggggga gaggctcgta cgattaacaa gggcgatgtc gtcgtcatcc
    12781 caaacggcac cgcgcactgg ttcaaggaag ttaaaggccc gatcgactat tacgttgcca
    12841 aagtcagagc agctgactga ttgaggagac aaacatgaga agaatttttg ttttaacagg
    12901 tatcgccctc cttttgaccc tacctgcgaa actggtggca ggcaattcac cggaagaatc
    12961 accagctaca cacaaggccg atgccgtcat cgaccttgcg actaaacagg gagtggaaat
    13021 ggttcgaggc cagtggcgct acagcgatac aaagatcatc gaagtcgatt tcaaggccgc
    13081 aggcctggat ggccagcccg gcgatatgcc gatcaaggct tatgacttta caccccatgc
    13141 cggcggggcc gacttcgacg acgccagctg ggaagtcttg gatccaacaa ctttggataa
    13201 acgccgctcc gctggaagac tcgccttcaa ctggtacagg atcaagatta gcgtcccgga
    13261 gcgaatcggt aatttcgatc cagcaggctc aacagtggtc ttcgctacct ctgtcgacga
    13321 ctatgccgaa atctgggtcg atggagagtt gccgcgcgct gccggacaga gcggcggttc
    13381 ggtcatcaaa ggttggaacg cggagaaccg gctggtcgtc ggacgcgacg tcaagcccgg
    13441 ccaaaagatc cagctcgcga tcttcggcat caacggccca gtctcgaatc cgccgaccaa
    13501 ctatatctac atgcgctatg ccaagctcga atttcataag atcgcggccg gcccgatcgc
    13561 tgtgacaccg caagaagtca acgtggaggt ggtgcggctc gatccggcaa tcgacgccat
    13621 cgttccgccc aatcccaaaa ttttcaagct cgccgaaggc tttcaattca ctgaggggcc
    13681 aatctggacg ccgagcggcg atcttctctt cagcgacccg aatgccaata aaatttacaa
    13741 gtacgccgct aacgaaacgc tctcgctgtt caagaacaac agcggttact ccggcaagga
    13801 tgtcgctgat tatttccaac ccggctccaa tggtttgact ctagacccgg aagggagact
    13861 gacgattaac cagcatggca acaggcgggt cgtgcggctg gaaaaagacg gaaagctaac
    13921 ggttctcgcc gaccgcttcg acggcaagcg gcttaacagt ccgaatgatc tggtctatcg
    13981 ctccgacgga acgctcttca ttagcgatcc tcctttcggc ttgccgaaat tctacgacga
    14041 tccacgcaaa gagcttccct acagcggcgt gtttgccctc tataaaggcc aactcaagct
    14101 ggtgagcacc gatctgaaag gtcccaatgg aatcgccttg tcgctggatg aaaaatatct
    14161 ctatgtcggc aactgggatc cggagaaaaa gatcgtaatg cgctatgtgg tgaacgccga
    14221 cgcgacgctg tccgatggag aagtcttctt cgacatgacg agcgcgccgg gacaagacgc
    14281 gatcgacggg atcaaggtcg atcagcaagg caacctctac gtctccgggc ccggcggctt
    14341 gtggattctt tcggcagcgg gcaaacatct cggcacgatc aaggcgccga agcatccgca
    14401 taacctcgct tggggcgggg aagatggtaa aacgctctat ttgacggcgc agagcgcgct
    14461 ttaccgcatg cccctcaaca tttccggtat ccgtcccgca gatggaatga gcaaactcgc
    14521 cgcgcagcgt taatgtcaaa agaatagaac tggccagcaa cgacacgaat agcgacatgg
    14581 ggtcgataag agcgaaagaa ggcagatcgc caggcgacct ctggaagctg ccagaaatat
    14641 aattgcggag aaagggagga ttcatgacag agagtcgacc attgaacatc cccgtcatat
    14701 tgggaaccac gcgcaaggga cgcatgagtg agcacgccgc ccgcttcatg gccggacaga
    14761 tcgaaaaacg caacggaatc aaaaccgagc tgatcgatct ttcccagctg ccaatgccca
    14821 tcgatgatgc cggcgaggca atcaaggatg cggccttttc agaaaagatg acgatggctg
    14881 atgcgctggt gatcgtgacc ccggagtaca accacagttt tccgggactg ctcaaacata
    14941 tcctcgacag ttgtttgaaa gaatacattc acaaggctgc cggtctcgtc ggcgtgtcgg
    15001 ccggtccttt tggcggcgta cgggcgattc aagactttct accggtcatt cgcgagctgg
    15061 gcctggtcaa tattttctgg gacgtcaact tcggcaacgt cagcaaggtc ttcgacgact
    15121 ccggcaagct gcttgacgcg gcctttattt cacgcgccga caagttcttg aacgagctgg
    15181 tctggatggc gagaaccttg cggtacgggc gggatgatat tgctctggac aattaacgag
    15241 aggacaagat ggctaagcaa aagagtacga tgatttgtcc gaactgtggc aaccagatga
    15301 accaccatgc cgacaagtta gtcgatccgg tgaagccgga agacctgcgg caccgggata
    15361 cggctctcgg cggtgtcatt gaagaaatac acggctgtcc cgggtgcggc gccgccgcat
    15421 cgcggagggc ggtgtgagcg ggagtaatat ttccatggct tcggatacta tttttgaaat
    15481 aggtaaaaac cggattgagg cgctcagcga tggcatattt gc
//
LOCUS       DASUVS010000074         6883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1555844, whole genome shotgun sequence.
ACCESSION   DASUVS010000074 DASUVS010000000
VERSION     DASUVS010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555844"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VF182_04385"
     CDS             <1..183
                     /locus_tag="VF182_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436344.1"
                     /translation="VRKDPNTYAWTWSSHIDFEDGHEIKFTSQRSFPTHLEAEEYMRQ
                     FARAHIDSRLRGMQQF"
     gene            312..683
                     /locus_tag="VF182_04390"
     CDS             312..683
                     /locus_tag="VF182_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436345.1"
                     /translation="MNSTKEWKIKQIISAQRGWKAVHCSQSDNGQLAMFERAIVCWAL
                     VEQHGGSDGGRTEVRGMEQRFDDLIVAGDVIKAKVLGADEVDCNEYFLGYDDPDAHRE
                     SQYWIGEAYRRLSKEKEQRAK"
     gene            1048..2595
                     /locus_tag="VF182_04395"
     CDS             1048..2595
                     /locus_tag="VF182_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6436346.1"
                     /translation="MYEKHFGFTKLPFSVAADPGNFYLSSTHQEGLTNLHYGVQSRKG
                     LIAVIGEPGTGKTSLLRMFVASAPPNIRTGIIYDPRISWKTMLRLILGKCGINAPRSD
                     AEGMMRRLETHLKRELKQQRSVVLLIDEAQTLSDDLLEQLRLLSNLESDQQKILQIVL
                     AGQCELEEKLSEAKFAALQQRIALRLQLTPLNAEEVDGYIKFMLQKAGYQGQELFGKR
                     AVERITDCSRGIPRLINSLCDNALLASYRAFEQSVPAERIDKAARDLKIQIQQSDPPA
                     AAESPSAAGGQFDFGDQQLGNDDSWQEELDASLADYEEAPKRNPGPSRPHDARLFMFL
                     ALILITAAALIFFRSGSDHAQGDRSAIGQANMDEASFPAPVEEHQQQAPLMASAFDSV
                     PSLAATKREAAGGHARVFVHTPNYRNEPVIKEVGKALQTAGYGTPEMRIATGKTGGDV
                     RFFFPSDRGEANTVKFLIEAELRKRGFPVSLQLLERDGRKFQHAAPGKIEVWLPPLPD
                     HSRVNNS"
     gene            2905..3804
                     /locus_tag="VF182_04400"
     CDS             2905..3804
                     /locus_tag="VF182_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436347.1"
                     /translation="MTKLIIQPHGRLNDWVADEKGYFHEEGLDYELNVEGSRKNTPPL
                     ASLGASESLADNRFGAFERYAEGHGRKGKNAGDVSCACHWTINQAAKVEEGIMWAKAY
                     SVCEAAIVVPGDSDAFQPGDLAGKDIAVGYHSGSHFSALQALEVFLTREDIALKFVGT
                     SWSRVDAALARKVPGVNAWGPQLYLLEQHGFRRVVSTTFMMAFMFPPDADEKSVERYM
                     RSLIRAQTDIDFEPERYKHYFINEIPERFRDHVDVRRFGVGERVVPQPYSQAIFDRTQ
                     AWLRERKLFDIASDAGVSGQEAV"
     gene            complement(3942..4775)
                     /locus_tag="VF182_04405"
     CDS             complement(3942..4775)
                     /locus_tag="VF182_04405"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX6436348.1"
                     /translation="MNDDVFTGQWTQLRGSLKSWWGRLTDDDLDRISGQKDRLVGLVQ
                     EKYGQTREQAEREVEQRLREFGGGGGIGAKLKSGVSEIGSNLSNKAREASSVVSSRVD
                     SARAYLHERSGNELLDDVVDAIQRYPVYASFVGLGVGYILGKGVSGAARLERLGRRKK
                     DELLGLVGETYQQTRAAAGRQAEQFSDSDMAAKLKVGASELRANLSKKAGEAVERLSP
                     TMESARSYVRDTNLDEFTGDLRNVIRKYPIYACLIALAVGVLTKGGVASRDRYQDRAD
                     W"
     gene            complement(5143..6147)
                     /locus_tag="VF182_04410"
     CDS             complement(5143..6147)
                     /locus_tag="VF182_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436349.1"
                     /translation="MRRDTSIIAALFAIVWLLFAQTGFSQTKPLKKIRVGVPSVGMGN
                     IIIFVTKEGKLFEKYGLDAEVITVMGSGIGSKALISGNLDIIPIATPTVIAADLAGAD
                     MAILAHTMPAVVHALMVRPEIKKPEDLKGKKIGVSSLGSLTDFLVRSIAKKKGLNPDR
                     DITLITTGGSDTERVMALKAGAVEATAVSHPGYGLARKMGFSMLWDSAKELDYPWMEI
                     TTRRAAIKKDRELITNYMKAHLEGIALFKTNRDFGIKVIKKYLKTPDDDLVGESYDIF
                     SKMFLPTPYPNHPGMKISYEYVAMTRNDIWNHKPEEFTDSSFVEELDKSGFIKKLYEK
                     "
     gene            complement(6305..>6883)
                     /locus_tag="VF182_04415"
     CDS             complement(6305..>6883)
                     /locus_tag="VF182_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6436350.1"
                     /translation="RPIAPPRLAWEIWEALKKEDWLLVAGAFRGWPSRLWTWDKPGLF
                     LGGYGGGGLGYGPAASVGASLPYRGTGKICVNLQRDGEMLYTPSALWTAANTGVPLLT
                     VMTNNRVYYNDVEHQEKVAIHRGRPVENKLVGMEMAKPPVDFANLARTFSVHGEGPIT
                     EPDQIRPAVERAIKVIKEEKRPALVDVYMQMV"
BASE COUNT         1593 a         1879 c         1833 g         1578 t
ORIGIN      
        1 gtgagaaaag atccgaatac ctatgcatgg acatggagca gtcatatcga ttttgaagac
       61 gggcacgaaa tcaaattcac cagccagcgc agtttcccaa cccatctgga ggctgaagaa
      121 tacatgcgcc aatttgcccg agcgcatatc gatagtagat tgagaggaat gcagcagttc
      181 tagcgtcagt agacctcgct tttcctccac ccgcccaagt tagcattgca tcggccctca
      241 tcgctgaatc cgtaactggt tttgatggca ggttcagatc gaccgagagg cgcagccgag
      301 ccagaggctt tatgaactcg accaaagaat ggaagatcaa acaaatcatc tcagcacaga
      361 gaggttggaa agcggttcat tgctctcaat cagataacgg ccagcttgcg atgtttgagc
      421 gggcgattgt ttgctgggcg ttggtcgaac agcatggcgg cagtgacgga ggcagaaccg
      481 aagtgcgcgg aatggagcag cggttcgacg atctcatcgt cgccggtgac gttatcaaag
      541 ctaaagttct tggcgcagat gaagtcgatt gcaacgagta ttttcttggc tatgacgatc
      601 cggacgctca cagagaatca cagtattgga tcggcgaagc gtaccgacgc ttaagcaaag
      661 aaaaggaaca aagagccaag taagtgttgc cccgacgaag ttcgtcgccc agtcaactta
      721 agcgcgccgg tacggagcct ttgagctggg ttttcacaaa gcaggtgtag ggccttcaag
      781 atgaagcgta tttaggctct cggccagctc tcccactgga agagattgtg atgaagccaa
      841 tgagctggct gagcttgact cggcgcttgt acctgctaga tttttgttaa cgccgtcact
      901 acatgctcac agccacgcgc gaatagattc ccactatctt aatcactacc cgttgttctc
      961 gttcgagcag cgctgttgat gcaataacgc agatttctcc cggcaaggag gtgcgacttc
     1021 ggactgctgt accgaaacaa acatcctatg tatgagaaac atttcgggtt tacaaaactt
     1081 ccctttagcg ttgctgccga ccccggcaat ttttacttga gctcgactca tcaggaaggc
     1141 ctaacgaatc tccattacgg cgtccagtcc cggaagggtc tgattgcagt aatcggcgaa
     1201 cctggcacgg gaaagacctc gctgctgagg atgtttgtgg ccagtgcgcc gccgaatatt
     1261 cgcaccggga tcatctatga tcctcgtata agctggaaaa cgatgctgcg gctgatcctc
     1321 ggcaaatgcg gcatcaacgc gccacgatcg gatgccgaag ggatgatgcg gcggctggag
     1381 actcatttaa agcgtgagct aaaacagcaa cgaagtgtcg tgttgctaat cgatgaggcg
     1441 caaacgttaa gcgacgatct gctcgagcaa ttgcggctgc tgtcaaatct tgaatctgac
     1501 cagcaaaaga tcctgcagat cgttttggcc ggacaatgcg agctcgaaga aaaactctcc
     1561 gaagcaaagt ttgcggctct gcagcagcgg atcgcgctcc ggcttcagct cacgccttta
     1621 aatgcggaag aggtagatgg ctacataaag ttcatgctgc aaaaggccgg atatcaggga
     1681 caagagctgt tcggtaagcg tgcggtagaa cggatcactg attgttcccg agggattccg
     1741 cgcctgatca actcactttg cgataatgcg ctgctggcta gctaccgcgc tttcgagcaa
     1801 tccgtgccag ctgagcgcat cgataaagct gctcgagatc ttaaaatcca aatccaacag
     1861 agcgatccac ccgctgctgc cgagtcgccg tcggcagcag gcggacaatt tgattttggt
     1921 gaccaacaat taggcaacga cgattcgtgg caagaagaat tggatgcgtc tctcgctgat
     1981 tatgaagaag cgccgaaacg gaatccagga ccttctcgtc cccatgacgc gcgcttgttc
     2041 atgtttctgg ctcttattct catcaccgcg gctgctctga tcttcttccg atccggcagc
     2101 gaccacgcgc agggcgaccg atcggccatc ggccaggcaa acatggatga ggcgagtttc
     2161 cccgctcccg tcgaggagca tcagcagcaa gccccattga tggcgagcgc tttcgattct
     2221 gttccgagtc ttgcggcgac aaaaagagaa gcagccggag gtcatgcccg agtttttgtt
     2281 cacacgccaa attaccgtaa cgaacctgtt atcaaagaag tcggcaaggc gctccagacg
     2341 gccggctacg gaactccgga aatgcgcatc gcgacgggaa agaccggcgg tgatgttcgg
     2401 ttcttttttc cgagcgatcg aggggaagct aatacggtga agtttttgat tgaggcggag
     2461 cttcgtaagc gcggcttccc ggtatcgctg caactgcttg agcgcgacgg cagaaaattc
     2521 caacatgccg cgcctggcaa gatcgaggtc tggcttccgc cgctaccgga tcattctaga
     2581 gtgaataaca gctaactacc ggcataacgc ctgctatcaa gcagcacgac tatctcaacc
     2641 atgcgcagcc tacatccaac gtcaacgaca cggagacaat cggttctcaa cgtaagctca
     2701 ctgaataaac tccgaagggc gatggcgcag acgattgagg cattacccaa tgcccgcgat
     2761 cgagccgtca attattccca gatgcaggct tgatcttcaa gcagtctcct acgactgtca
     2821 gagaagttcc cgccagaatc aagtgcagta tttgcaaagc tgacaagtgc tgaagtagca
     2881 tctggcagag aggagacaac ccgtatgacg aaattgatca ttcagccgca tggcagactg
     2941 aacgattggg tagccgacga gaagggctat ttccacgaag aagggctgga ttatgagctc
     3001 aatgtagaag gaagccgcaa aaacacgccc ccactcgcct ctctcggggc aagcgaatcg
     3061 ttagccgata accgctttgg tgcctttgag cgctatgccg aaggacacgg gcgcaagggc
     3121 aaaaacgcgg gcgatgtcag ctgcgcctgt cattggacca tcaatcaagc cgcaaaggtt
     3181 gaagaaggca tcatgtgggc taaggcttat agcgtttgtg aagcggccat cgttgtgccg
     3241 ggcgattccg acgcattcca accgggagat ctggcaggca aagacattgc ggtgggctat
     3301 cattcgggaa gccatttctc ggctctgcaa gccttggaag tctttctgac gcgtgaggat
     3361 attgcgctca agttcgtcgg gacttcctgg tcccgggtcg atgcagcgct ggcacggaaa
     3421 gtacccgggg tgaatgcctg gggaccgcag ttgtacctat tggagcagca tggttttcga
     3481 agagtcgttt ccacgacgtt tatgatggcg tttatgtttc cgccggacgc ggatgaaaaa
     3541 agtgtcgagc gctatatgcg ttcgctaatc cgggctcaga ccgatatcga tttcgaacct
     3601 gagcgctaca agcattactt catcaacgag attcccgagc gctttcgcga tcatgtggac
     3661 gtccggcgtt tcggcgtcgg cgagcgcgta gtcccgcagc cttatagcca ggcaatcttc
     3721 gacagaactc aggcttggct gcgcgaacgc aaacttttcg atatcgcctc ggacgccggt
     3781 gtctctggcc aagaagcagt gtgaccttta aattttcgcc gcatcgctca gctttgttcc
     3841 cgttcttaat gatggcaaat cgggggcagg gtcgactgag gtccgggagg ctgcgttcgc
     3901 gggcagcctc ccgggaatac gatgggatta aagtacgcga attaccaatc cgcgcgatcc
     3961 tgataacgat cgcgcgaagc cacgccgcct ttcgttagga cgccaacagc caaagcgatc
     4021 aggcaggcat aaatcggata cttccggatt acgttgcgca ggtcgccggt aaattcatcc
     4081 aggttcgtgt cgcgaacata agaacgcgcc gactccatag taggcgataa ccgttctacg
     4141 gcttcacccg cttttttcga taaattcgcc ctcaattcac tggcgccaac cttcagctta
     4201 gcggccatat cgctgtcgct gaattgctcc gcttgccgtc ccgcagcagc gcgggtctgt
     4261 tgataggtct ccccaaccaa ccccagcaat tcatcttttt ttcgacggcc taatcgttcc
     4321 agacgggcgg cgccggacac gcccttgccc aagatatagc caacgccgag tcctacaaag
     4381 gaagcgtaaa ccggatatcg ctgaattgcg tcgaccacgt catctagtaa ttcattgccg
     4441 cttctttcgt gtaaataggc acgggcggaa tccaccctgc tcgacacgac gctagacgct
     4501 tctctcgctt tattcgatag gttggatccg atctcgctga cgccggattt gagcttagct
     4561 ccgattcctc cgccaccgcc gaattctcgc aagcgttgct ccacctcacg ttctgcctgt
     4621 tcgcgcgttt gtccgtattt ctcttggact agtcccacca gccggtcttt ttgaccgctg
     4681 attctatcga gatcatcatc ggtcaatcta ccccaccaag acttcagcga tccgcgcagc
     4741 tgcgtccatt gtcctgtgaa tacatcgtca ttcatgggaa aatacctcca ttcgtatttg
     4801 catgacggct cgagaaattt gagggcaaag cgtatgccac taagccgaac cacaaatcac
     4861 gatgatcacg actgttttta gagtgttgtc cgaagtttga cacacggagt ttccagatgt
     4921 ctcacaagcg actcggcgag ccactccagt actgctgctc gatcattact aaaacgaccc
     4981 gccgattcag attcatgaaa tgtgtttttc tgctggaatg ggctccgact tctgacaacg
     5041 tcatggcggc gcctgtccta tacggcgctg caccagcgaa gcgaacagtg ccttggcaac
     5101 cgaaagcttt agtcgtttgt tccgcacaac ttaaggagca acttactttt cgtagagctt
     5161 tttgataaat ccgctcttat ccaattcttc aacgaagctc gagtcggtga actcctcggg
     5221 cttgtggttc catatgtcgt tgcgggtcat ggcgacatat tcgtagctga tcttcatccc
     5281 aggatgattg ggatacggcg tgggaagaaa catttttgaa aagatgtcgt aagattcacc
     5341 aaccaaatca tcatcaggcg ttttcagata ctttttgatg actttgatgc cgaaatcgcg
     5401 gttggttttg aacaacgcga tgccttctag gtgcgccttc atgtaatttg tgatcagctc
     5461 ccgatctttt ttgatcgccg cccggcgggt ggtgatttcc atccacgggt agtcgagttc
     5521 cttggcggaa tcccatagca tgctaaaccc catttttcgc gctaagccgt agccgggatg
     5581 ggacaccgca gtggcctcga ctgcgccagc cttgagcgcc atgacccgct cggtatcgct
     5641 gccgccggtg gtgatcagag tgatgtcgcg atcgggattg agtcccttct tttttgcgat
     5701 cgagcggacc aaaaaatccg tcaacgatcc caagctggaa acgccgatct ttttgccttt
     5761 caagtcctcc ggctttttga tctccggccg gaccatcaag gcgtgaacca cagcgggcat
     5821 cgtgtgcgcc aagatcgcca tgtcggctcc cgcaagatcg gccgcgatga cagtcggcgt
     5881 ggcgatcgga ataatgtcca ggttgccgct gatcaaagct ttcgagccga tgccggagcc
     5941 catgaccgtg atcacctcgg cgtcgagacc gtacttctca aacagcttac cctctttggt
     6001 gacgaaaata atgatgttgc ccatgccgac cgacgggacg cccacccgga ttttttttag
     6061 gggtttggtt tgtgaaaagc cggtttgagc aaaaaggagc cagacaatcg caaacaaagc
     6121 agcgattatg gacgtgtctc gcctcatgaa acatcctccc agattaacct atgccgtatc
     6181 tccaccgtcc cgtgaacggt taccaattat tttcatagga gttgtgtatt ttgtctatcc
     6241 gtttcttctt aacggttgga acggcggaaa gttggaaccg ctccaagtac tggaacactt
     6301 cgaactaaac catctgcata tagacatcga ccaatgccgg ccgcttctct tctttgatga
     6361 ccttgatggc ccgttccacc gccggccgaa tctggtccgg ttcggtgatc ggtccctcgc
     6421 catgaacact gaaggtgcga gccaggttgg caaaatcgac agggggcttg gccatctcca
     6481 tacccacgag tttattttcc accggccggc cgcggtgaat cgcgactttt tcttgatgct
     6541 ccacgtcgtt gtagtaaacc cggttattgg tcattaccgt cagcagcggc acaccggtgt
     6601 tggcggcggt ccagagggcg ctcggggtgt acagcatctc gccgtcgcgc tgcaggttga
     6661 cgcagatctt gcccgtgccc cggtacggca gggatgcgcc gaccgatgca gccggcccat
     6721 agcccaatcc gccgccgcca tagccgccaa gaaacagccc gggcttgtcc caggtccaca
     6781 gacggctcgg ccagccgcga aaggctccgg cgaccagtag ccagtcttct tttttcagcg
     6841 cttcccaaat ttcccacgca agacgcggcg gcgcgatggg ccg
//
LOCUS       DASUVS010000075         7426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_463235, whole genome shotgun sequence.
ACCESSION   DASUVS010000075 DASUVS010000000
VERSION     DASUVS010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7426
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463235"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(407..1462)
                     /locus_tag="VF182_04420"
     CDS             complement(407..1462)
                     /locus_tag="VF182_04420"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017956537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6436351.1"
                     /translation="MKVNEQKLNEVVEKAISDLAAGYGGVMVALGDKLGLYRAMAGQG
                     PLSSSEVAKLAGCAERYVREWLNSQAAAGYVVYHPSSETYELTAEQAMVLANEDSPLF
                     LPPAWEVPASMWFDEEKTIEAFRTGRGIGWDEHNSRLYRGVAAFYRNAYQGQLVSDWL
                     PALKNGTEKLNAGATIADVGCGHGHSTIIMAEAFPGSRFWGFDIHPDSIEAARGNAKK
                     RGVDDRVTFAVADAKNYPAQGFDLICFFDCLHDMGDPVGAARHAREALSPNGSMMLVE
                     PYANDRVEDNLNPIGRLYYSASTTLCCAHSLSEEGMALGAQAGEKKLTKVLKEAGFQT
                     IRAAKKTPFNLIVEAQA"
     gene            1581..2630
                     /locus_tag="VF182_04425"
     CDS             1581..2630
                     /locus_tag="VF182_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6436352.1"
                     /translation="MKGGKKSVGVSLLAIPEVMASTLIGLYDVLNSFTLLSTYNDALP
                     SEPPFTVEIVGPSRGLTHTSSGMPIPAGRSIDDVDSTDVIIVPSVIVARGEWLKGKHP
                     SIVRWLTEMHAKGAQLCSTCSGVLLLAETGLLEGKEATIHWIYAPTFQRNFPGVRLRV
                     EKALIAIGENNQFIMSGASTAWHDLILCLISQHAGPTAAQAIAKFFAFQWHADGLSPY
                     VMFEPAMDHGDAVVQETQKWLDKHFSVPSPIEQAVRRSGIAERTFKRRFKNATGHTPI
                     EYVQRLRVDEAKRRLERTTAPIDEIGWQVGYEDPTFFRRLFKRLTGVTAGAYRRRFRI
                     PESVPLAKMKNGIRL"
     gene            complement(2746..3282)
                     /locus_tag="VF182_04430"
     CDS             complement(2746..3282)
                     /locus_tag="VF182_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6436353.1"
                     /translation="MKSRHLAFRACVALTLIGAACAFNSSRKTIPGDWEQASYIERGN
                     YLVNHLGHCVGCHTPLAPDGESDMKFYLSGVPAKYAGTTVGRSQVAGFPGPRGARFYA
                     KNLTPDPETGLGRWSEEQFVQTFKNGVSPDGTKYAISPMEWNIYANMKEEDVRAIYRY
                     LRTIKPIPNKVPANIPPK"
     gene            complement(3294..3800)
                     /locus_tag="VF182_04435"
     CDS             complement(3294..3800)
                     /locus_tag="VF182_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436354.1"
                     /translation="MKWISTLLMILATSFLISPTARGRAETEEERIARGRYIATAIQG
                     CGCHTRERPDGSKDENWHYAGSPNPAPPAGPPANAGWSSPRWKKIYARNITPDRETGI
                     GNWTEADFIRSMKTGLTPDGRVLDAQMPWDAFQKITDRDLKSLWAYLRTIKPIKNTPP
                     ERIPAEQK"
     gene            3853..4254
                     /locus_tag="VF182_04440"
     CDS             3853..4254
                     /locus_tag="VF182_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6436355.1"
                     /translation="MFATELVLFELSQAQSGALRILIVAGVLLLCYSTAYADMEQGKR
                     IYRENCAPCHGESGKGDGVGARSLPVRPADHTNPAAMTSRTDAFLRDVIAKGGKAMGL
                     SSFMPAWEGIFKDKEIEDLVAYIRTLALPAK"
     gene            4847..6805
                     /locus_tag="VF182_04445"
     CDS             4847..6805
                     /locus_tag="VF182_04445"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX6436356.1"
                     /translation="MDGTITWMLIVAGAAIALLGVFLFVSEKELRKKRREIAGLLAKF
                     SRPNTGVVAGDYQNTLNKLEDANKELLEQLSALSSRLESTEHGFTQLETEKRRLEDSN
                     QQLNDEALALRQKLHSALKAHEDSRLDRAEFNDQLATMASELKATTAKLEQAKIRIEE
                     LENANDPSALSHAESLLTESRAEFENSLRALQRDLADREDKLQTLDTVKRQLLDLEEK
                     YNKALLDNRELQQEITRYQEQLSDADVASRDFGIIRPQMDDPHLLSESPNHQAQMQFD
                     MMALPENRYSSRHAAQTGTVLLSTASTGLSGMDGLESHGQRAGSRIALEYVEPGRNEN
                     KPLVGDLELRQSANDRTAQLSHLLAWVRLHGGDQYREQIDSLKDFTDLSEREREVVRD
                     IFLARADEAQKRGRDDAMLRYRSWAKNIVYHTRFADDGIEDLSQDEATTIRAGQGAIP
                     DRTATDMVPAITATNAAKAGLTTVQSDRIKSPAGNVALAIGVGLGLVLILLVQLFFDT
                     PAEITVASDEALTPAKSQSAELPPPAKSIGASQSTSLPENSRDEPSRPEERVRTKPDE
                     IKATVRNDNKEPAAKTTRPQSIFGAYTIVKSTPVYSEPNEGAALITSLDPGTQVNVVA
                     ARQGWYEIRSRSGRPPGFIRQGGASRNR"
     gene            7002..>7426
                     /locus_tag="VF182_04450"
     CDS             7002..>7426
                     /locus_tag="VF182_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6436357.1"
                     /translation="MELYSRIEGSGFPIIILHGLLGSSDNWRAITKRLNSSYKVFAVD
                     LRNHGQSPHSEIMTYPAMADDVRELLDHEKISECHLVGHSLGGKVAMQFAVTHPDRVS
                     KLVIVDIAPKAYPPSQRAILAALEQLHLQNFKAFGTIDAA"
BASE COUNT         1685 a         2088 c         1992 g         1661 t
ORIGIN      
        1 gcatatcagc acccttagac cacgcgattg tcagaccgtc aatgaaaaac ttctgtagtg
       61 tttgttcaga ggttcgaaaa tcggtcctgt gcgccacggc taagcgatag ttctttggtg
      121 ctgtcgatac atggccgaag aacctcttac ttctattccg catagttatt ccactccatt
      181 gggagattgg gtagttggta ccgtcccgac tgacaccctg gatgaaactc ttgagagact
      241 gctttccgtt caaggaccga gtcccgtttc cttatccggg tcagattggc agtagcaccg
      301 cgactgcgcg ctccgggaac accggctatc tgctatctgt aacgaacaga ggtagcgcgg
      361 catgcccgcg ctacctccac gatcgctaat ctctggatcg gctactctaa gcttgagctt
      421 cgacaatcag gttaaacggg gttttcttgg ccgcccggat agtctggaat ccagcttctt
      481 tgagaacctt cgtcaacttc ttctcgcccg cttgggctcc gagcgccatc ccttcttcgg
      541 ataatgaatg cgcgcagcag agcgtcgtcg acgcggaata gtaaaggcgt ccgatgggat
      601 ttagattatc ttcgacccga tcgttggcat agggttcgac gagcatcata gacccgtttg
      661 gcgacaacgc ttcgcgcgcg tggcgcgccg cgcccacggg atctcccatg tcgtgcaaac
      721 aatcgaagaa gcagatcaaa tcgaaacctt gcgcgggata gttcttggca tccgcaaccg
      781 cgaaggtaac acgatcgtcg acaccgcgct ttttagcatt tcctcgcgcg gcctcgatcg
      841 aatccgggtg gatgtcgaag ccccaaaatc gagacccggg aaacgcttct gccatgatga
      901 tcgttgagtg tccgtggccg cagccgacgt cggcgatcgt agccccggca tttaacttct
      961 ccgtgccgtt ttttagagcg ggtagccagt ctgaaactaa ttggccttgg tatgcgtttc
     1021 ggtaaaaagc ggccacacct cggtacagtc gcgagttgtg ctcgtcccag ccgatgcctc
     1081 gcccagtgcg gaacgcctcg attgttttct cttcatcgaa ccacatggac gccggcactt
     1141 cccaggcggg cggcagaaaa agcgggctgt cctcgttagc gagcaccatc gcctgctcgg
     1201 ctgtgagctc gtaagtctcg ctggacgggt gataaacgac ataacccgcg gctgcctggc
     1261 tgttgagcca ttcgcgcacg taacgctcgg cgcaaccagc cagtttggcc acctccgagg
     1321 agctcaaggg accctgtccc gccattgccc tgtacaagcc aagtttatct cccaaagcga
     1381 ccatcactcc gccataaccc gccgccaggt cgctaatcgc tttttccacg acctcattta
     1441 gcttttgctc gttgaccttc atcgttctat cctcctaagg tagatttgct ttcacgccag
     1501 tcaaaataat ctctagagcc tgtctgtaga agagggaaaa cggtcaaaag aggttcgttc
     1561 gtgccagagg gaatggagaa atgaagggtg gaaaaaaatc ggtcggcgtc agtctcttgg
     1621 ctattcccga ggtcatggcc tcgaccttaa tcggactcta cgacgtgttg aactcgttca
     1681 ctttgctttc cacttacaat gatgcattac cgagcgagcc gccgttcacc gtggaaattg
     1741 tcgggcccag tcgcggcctg acccatactt ccagcggtat gccgatcccg gcaggccgat
     1801 ccatcgatga cgtcgattcg accgatgtga tcattgtacc gtcggtcatc gtggcgcggg
     1861 gcgagtggct taaaggcaaa catccaagta tcgtccgttg gctaaccgaa atgcatgcca
     1921 aaggcgcgca gttgtgttcg acgtgctccg gcgtacttct gttggcggaa accggtttat
     1981 tggagggcaa agaagcaacc attcactgga tttacgcgcc gactttccaa agaaactttc
     2041 ccggtgttcg tttgcgcgtg gagaaagcct tgattgcgat cggcgaaaat aatcaattca
     2101 tcatgtccgg cgcgtccacg gcatggcacg atttgattct ctgcttgatc tcccagcatg
     2161 ccggtcccac agccgcccaa gcaatcgcta agtttttcgc ctttcaatgg catgccgacg
     2221 gcttatctcc ctatgtcatg ttcgaaccag ccatggatca tggcgacgcc gtagttcaag
     2281 agacgcagaa atggctcgac aagcatttct cggtgccgag cccgatagag caagcggtgc
     2341 gccgatccgg catcgcggaa cgcaccttca aacggcgctt caagaacgca accggtcaca
     2401 cgccgattga gtatgtccag cgcctgcgcg tggatgaagc aaagcggcgg ttggaaagaa
     2461 caaccgcgcc catcgatgaa attggctggc aggtcggcta cgaagacccg acgttttttc
     2521 gccgcttatt caagcgtctc accggcgtta ccgccggcgc atacagacgc aggtttagga
     2581 tccccgagtc tgtgcctctc gcaaagatga agaatggcat tcgattgtga tggcgtgatt
     2641 ccggcgtaat gatatcaccg cggctgtttg cagtttcaat ccccgccgca acaagttgtc
     2701 actcatcggc gccacggaca aaatcaatgc tcgtccagcg gcgtgctatt tgggcgggat
     2761 gttcgccgga actttattgg gtatcggttt gatcgttcga agataacggt agatggcgcg
     2821 aacatcctct tccttcatat tggcatagat gttccactcc atcggcgaga tcgcatactt
     2881 ggtgccgtcg ggactgacgc cgttcttgaa tgtttgcacg aactgctctt cgctccagcg
     2941 gccgagtccg gtctccgggt ccggagtcag attcttggca tagaagcggg cgccgcgcgg
     3001 tccggggaaa ccggccactt ggctgcgccc tactgtcgtg ccggcatact ttgccggcac
     3061 gccggaaaga taaaatttca tgtccgattc gccgtcgggc gccaacggcg tgtgacagcc
     3121 gacacagtgg ccgagatgat tcaccagata gttgcctcgc tcgatgtaac ttgcttgctc
     3181 ccaatcgcca ggtatcgttt tccgtgacga gttaaacgcg catgccgcgc cgatcaaggt
     3241 caaagcgacg caagcgcgaa aggccagatg ccgcgatttc atagtttctc cggttatttc
     3301 tgttcggccg ggattctttc gggcggcgta ttcttgatcg gcttgatcgt tctgagatac
     3361 gcccacaacg actttagatc gcggtcggta attttttgaa aggcatccca aggcatctga
     3421 gcatcgagca ctcggccatc cggagtgaga ccggtcttca tcgaccggat gaaatccgct
     3481 tcagtccagt tgccgatacc ggtttcccga tcgggcgtga tattcctcgc gtagattttc
     3541 ttccaacgcg gcgacgacca gccggcgttc gccggcggac cggcaggcgg cgcgggattc
     3601 ggtgaaccgg catagtgcca attctcatcc ttactgccat cgggtctttc acgtgtgtgg
     3661 cagccgcagc cttggatcgc agtggcgatg tagcggccgc gggcgatcct ttcctcttcc
     3721 gtctcagcgc ggccccgcgc ggtcggcgag atgaggaaag atgttgcaag gatcatcaaa
     3781 agagtagata tccacttcat cgatcgggcc tttccacaaa tctgatcatc gcgatgtagc
     3841 attgggtgga acatgtttgc aaccgaatta gtcctttttg aactcagcca ggcacaaagt
     3901 ggcgcgctgc ggattctaat cgtcgccggc gttttgcttc tatgctactc aactgcctac
     3961 gctgacatgg aacagggtaa acggatctat cgtgagaact gcgctccctg ccatggcgag
     4021 tcgggtaaag gcgatggagt gggcgcgcga tcgctgccgg tgcgtccagc cgatcatacc
     4081 aaccccgcgg ctatgacttc acgcacggat gcttttctgc gcgatgtcat agccaaaggc
     4141 ggtaaagcga tgggtttatc ctctttcatg ccggcatggg agggcatctt taaagataaa
     4201 gagattgagg acctcgttgc ttacatccgt accctggcgc tgcccgccaa atgaacggtc
     4261 tacggcgccg cccaacttat atcggttagt tgtttatagc ttttagtttt cggtctgaaa
     4321 actcccgccg cgatttcgcc accttttgct gttgattggt tgagacgatt gccgtgctca
     4381 ccggtcgcga gaggcgcgcg tggcaaagaa tcattctcgg ctttctgcgc gcagacaata
     4441 ttttctatcg gagagccggt atgtctcgca tcgctaacgc caatagcgct tgactgtcca
     4501 ccatttacaa ttttcgccag gattcacgat tttgtttatg gccagcgaat cggaacgcca
     4561 tcatgatggc ttgcccacgc gattagtcgc gcactatcaa cccttttagg agcttgacgg
     4621 agcaatcacg agaattggca catatttggc gttatcggct gcgggcaaat caactctggc
     4681 cccagccgaa ccttggttga accgttctga gaaccctaaa tctaaaatga ttctctacat
     4741 ctcttctcca gcgccaacag agcacgcctg gcttgcacga tcgaggcatc cgtacggcgc
     4801 taaatcttag gcttcagcag gttcaaatat cgatagggag accacaatgg atggcacgat
     4861 cacatggatg ctcattgtcg caggtgctgc catagctcta ctgggcgtct ttctgtttgt
     4921 ctctgaaaag gaacttcgaa agaagcgccg cgaaatagcc ggactgctcg caaagttttc
     4981 ccggcctaac acaggcgtag ttgccggtga ttaccaaaac accctaaaca aactcgagga
     5041 cgccaataaa gaattgctag aacaactctc ggctctgtcg agcaggctcg agtcgactga
     5101 gcacgggttc acccagctcg agaccgagaa gcgccggtta gaagattcta accaacagct
     5161 caatgacgaa gcattagccc tccgacagaa attgcattct gcattgaaag cgcacgaaga
     5221 ttctcgcttg gacagagcgg agttcaacga tcagcttgcc acgatggcaa gtgagttgaa
     5281 ggcgactacg gcaaagctcg aacaagccaa gataagaatc gaagaacttg agaacgccaa
     5341 tgatccgtca gctctttcac atgccgagag cttgctcacc gagtcgcgcg ccgagttcga
     5401 aaacagtctt agagcacttc agcgagatct ggccgatcgc gaggacaagc ttcagacatt
     5461 ggatacggtc aagcggcagt tgcttgatct cgaagaaaaa tacaacaagg cgctgctcga
     5521 caatcgagag ctgcaacaag aaatcactcg ctatcaagag cagctttccg atgcggacgt
     5581 cgcatcgcgg gatttcggga tcattcgccc acagatggat gatccgcatt tgctgagcga
     5641 aagcccgaat caccaggcgc aaatgcaatt tgacatgatg gcactgcctg agaaccgtta
     5701 cagcagccgc cacgctgcac aaaccggcac agttcttcta agcacagctt caacggggct
     5761 atccggaatg gatggcctag agagtcatgg acaaagagcg ggttcgcgta ttgcactcga
     5821 gtatgttgaa cccggcagga acgaaaacaa gcccctcgtc ggcgatctgg aactgcggca
     5881 gtcagcgaat gaccgaaccg cccaactgtc ccatctgctc gcctgggtcc ggcttcatgg
     5941 cggcgatcag taccgagaac agatcgattc acttaaagat tttacagatc tttccgaacg
     6001 tgaacgagaa gtggttaggg atatcttcct ggcccgtgcg gacgaagcgc aaaaacgcgg
     6061 acgagacgat gcaatgctgc gctatcgcag ttgggcaaaa aatatcgttt accatacccg
     6121 ctttgccgat gacgggatcg aggatctatc acaggacgag gctacaacaa tcagagccgg
     6181 ccagggcgca atccctgatc gaacggccac ggatatggtg cctgcgatca cggccacaaa
     6241 cgcggccaaa gccggtttga cgaccgttca atctgaccgg atcaagagtc cggcaggaaa
     6301 tgtcgcgcta gcgattggcg taggcctggg tttggttctg attcttctgg ttcagctttt
     6361 ctttgataca ccggcggaga tcactgtggc ctcggatgag gccttgacac ccgccaaatc
     6421 tcaatccgcc gagcttccgc caccggcgaa atccataggt gcgagtcaat caacaagtct
     6481 tcctgagaat agccgcgatg agccttcacg gcctgaagag cgcgtcagaa cgaaacccga
     6541 cgaaattaaa gctaccgttc gcaacgacaa caaagaaccc gcggccaaga ccactcgacc
     6601 acaaagcatt tttggcgcgt atacgatcgt taagtcgacg ccggtttaca gcgaacccaa
     6661 tgagggcgcg gcgttgatta ccagcttgga tcccggcact caggtcaacg tagtggcagc
     6721 gcgccaggga tggtacgaga ttcgctctag gagcggccgt ccaccgggat ttatcaggca
     6781 aggcggtgcc tcgcgcaatc gttgatggct atcgcaaaag gtcggtatca aagggcgacg
     6841 cgagtaacga tgcgaaatct ctcgcggtaa atgcggggag tcgaaccgca agaacctaac
     6901 ctcgctcatt ccacatcccg gcgcgcgtca tctcagcgga aacctccgcg cggctccggc
     6961 tttcatttaa cagccattct tgccagactt tagttcctga catggaactc tactctcgga
     7021 tcgaaggatc gggtttccct attattattc tgcacggtct cctcggctcg tcagataact
     7081 ggcgcgcgat caccaagcgg cttaattcca gctataaagt ttttgccgtg gacctccgca
     7141 atcatggaca gtcgccgcac agcgaaataa tgacgtaccc ggccatggcc gacgatgtgc
     7201 gcgaactctt ggatcatgaa aagatctcgg aatgccatct tgtcggccac tcgctgggcg
     7261 gcaaggtcgc catgcagttc gccgttaccc atccggaccg ggtgagcaaa ctggtaatcg
     7321 tcgacatcgc gccgaaagcc tacccgccat cgcagcgcgc gatactagcg gcgctcgagc
     7381 agttgcacct gcaaaatttt aaagccttcg gcacaatcga tgcggc
//
LOCUS       DASUVS010000076         4629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_682408, whole genome shotgun sequence.
ACCESSION   DASUVS010000076 DASUVS010000000
VERSION     DASUVS010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682408"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1156)
                     /locus_tag="VF182_04455"
     CDS             complement(167..1156)
                     /locus_tag="VF182_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436358.1"
                     /translation="MLKLSATTLGVVFSLIFFCSPGDAKWVVAWSAVSALNSPFWVMQ
                     DAGFFKKEGLDVELVYIASSPTVARATLAGDIVISGANSQVIVDVGLNGGDLVAMGAI
                     TNVVAFYVMAAPEIKNVTDLKGKVVGVTRFGASTDFGMRMLLSKYGLEAAKDVPFIQI
                     GGMPELTAALSNRTVFAAPMSQPMVYLAQKAGMRMIANLAKEDIPFMHIGLTTSKKWI
                     RENRADAKAFIRAYGRAIHFMHNHKEETRAIFARYTRINDPGMLDGSIQYGYDFMEKV
                     PLVRSQAFQVTLDQIARNNPKAKQVKPDQFFDNSLVQEVVDEGFFTKLWGKKL"
     gene            1312..2505
                     /locus_tag="VF182_04460"
     CDS             1312..2505
                     /locus_tag="VF182_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX6436359.1"
                     /translation="MSIRGKAAIVGIGETPTDRLGSKPGEPRKSSAQYLAWAMRLALE
                     DAGLTLKDLNGQGLGVTIPTAYPQPFWPEEVAEILGITPGLLLAGSTGGAGAISLLGG
                     MSAAINSGLIDIALCIGAAAPFSEHFGGGIQPGDMRDFEIPFGTMGPNSKIAMVMRRH
                     MHQYGTTLDHLGKIAVAGRYHASLNPSAYLRKPITIQDYKDSRLVADPVRLLDCVLPA
                     NGGKAYIMASSERAKSLRKPPVFVRGFGERSNPSYGPRAGSDSLIMGVADAGKVAMEM
                     AGVKHGDINFLELYDDYIIVVYLQIEDLGFCAKGDLGFFERTDFTINGQLPIQTGGGM
                     INCGQPSTAGGTIHVIEAVRQLRGEGGQRQVRDAKIGMVTGLGVLPYGKNLGCCAVAV
                     LDREA"
     gene            2758..3189
                     /locus_tag="VF182_04465"
     CDS             2758..3189
                     /locus_tag="VF182_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEX6436360.1"
                     /translation="MTETDLQKPLPTITSEAKPFWDGAAKQKLMMQRCVDCRAYVWTP
                     RPSCFECGSENIQWQELSGKGKVYSFTVIRQVVGRAASQAFEKDIPYVIAWIDLDEGP
                     RMISNVIGCPVEAVKLGMKVSVIFEQQSADIWLPKFRPVSS"
     gene            complement(3223..4185)
                     /locus_tag="VF182_04470"
     CDS             complement(3223..4185)
                     /locus_tag="VF182_04470"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6436361.1"
                     /translation="MLVDVILSVLLVLYLAGCSSTTSDLSISTTNGTGASEQIPAKLL
                     KPDGAGVFPAVVIMHDCSGLGSRSSGAPDRWANQLLTYGYVVLIPDSFTTRGHASGVC
                     TNPSPSRTEVSPFRRTRDAYAALSYLRTLPYVDRARIGLMGGSHGGSTTLASMVSPES
                     DREPLARDKRTGFAAAVALYPNCAIRLGSWRPESRSGMNGPTLSYVGAYKAIAPLLIL
                     IGENDDWTPAEPCRKLTEAAVQEGYPVAIKIYPGAHHSFDSSNPVRHVAARINTNSPT
                     GRGATTGGDPKAWADSIREVITFFGRHLGKEKSKFAGKERDGNN"
     gene            complement(4265..>4629)
                     /locus_tag="VF182_04475"
     CDS             complement(4265..>4629)
                     /locus_tag="VF182_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX6436362.1"
                     /translation="DPMSHYEHLKAQDELMTNFLKVMADHRLDAIVHKAIEHQPTLIK
                     DGVNPPFVDQKGAPHLNTFLVFVPTIVVPAGFTRDNLPAGVCFTGRPYDDGSLIKFAY
                     AYEQATHHRRPPASTTGL"
BASE COUNT         1046 a         1279 c         1292 g         1012 t
ORIGIN      
        1 tcctttgtgt cactagctca aaagtttcac ggctcgcgca aagtaaagcg gcttaccaat
       61 ctccccggca gcaaaaacac tcgggtcagt cgtctagttg gaatcgttgt tcctgccttg
      121 cgtctttgcg ggaaataatt cgatcaagtt gcgactttgg ccatgttcac agcttctttc
      181 cccacaactt agtgaagaag ccctcgtcga caacttcctg aaccagactg ttgtcgaaga
      241 actgatccgg cttgacttgc ttggctttag gattgttcct ggcgatttgg tccagagtca
      301 cctgaaatgc ctgggatcta accagaggga ctttttccat gaagtcgtaa ccgtactgaa
      361 tgctgccatc gagcattccc ggatcgttaa ttctagtata gcgcgcaaag atagcgcggg
      421 tttcttcttt gtggttatgc atgaaatgaa tggcgcgccc ataggcgcga atgaatgcct
      481 tcgcatctgc gcgattttcc ctgatccatt ttttcgatgt ggtcagaccg atgtgcatga
      541 acggaatgtc ttcctttgcc aagttggcga tcatgcgcat ccccgctttc tgagctagat
      601 aaaccattgg ttgcgacatg ggcgccgcga aaacagtcct gttcgaaagc gccgcggtaa
      661 gctcgggcat gccgccgatt tgaatgaaag gaacatcttt ggcggcttcg agaccgtact
      721 tcgacaggag catgcgcatg ccgaaatcag tcgaagcgcc gaagcgggtc acgcccacaa
      781 ccttgccctt gaggtcggtt acgtttttga tctcgggcgc ggccatcaca taaaacgcaa
      841 cgacgttggt gattgcgccc atggcgacaa gatcgccgcc gttgagtccc acatcgacga
      901 tcacctggct gttcgcgcct gatatgacaa tgtcgcccgc aagagtcgcc cgcgccacgg
      961 tcggcgagct ggcgatgtaa accagctcca cgtccaatcc ttcttttttg aaaaagccgg
     1021 catcctgcat aacccagaac ggcgagttca aggcgctgac ggccgaccat gcaacgaccc
     1081 atttagcatc accggggcta cagaaaaaga tcagcgagaa aacaacgccg agcgtcgttg
     1141 ccgagagctt taacatataa acaaaccccc ttgcattggg ccacgctact gggaatccac
     1201 gattaccaat gctgtacaga acagcgttgc gttgtgtcaa gcgatgaaat cagactgagt
     1261 atgcacttga agttgcattt ctctcttgag ccacactata acgattaaac tatgagtatt
     1321 cgcggcaaag cagccattgt cggaatcggc gagacgccga cggatcgtct cggttcaaaa
     1381 ccaggcgaac cgcgcaaatc gagcgcgcaa tatttggctt gggcgatgcg tcttgcgttg
     1441 gaagacgcgg ggctaacgct caaggatctc aacgggcaag gtctgggcgt aaccattccg
     1501 accgcgtatc cacagccgtt ttggcccgaa gaggtcgcag agattctcgg catcacaccc
     1561 ggacttctgc tcgcgggcag cacgggcggc gcgggcgcga tctcacttct gggcgggatg
     1621 tcggcggcga tcaactcagg tttgattgac atcgctttgt gcatcggcgc ggcagcacct
     1681 ttctctgaac acttcggcgg cggtattcaa cccggcgaca tgcgcgattt cgaaattccc
     1741 ttcggcacca tgggcccgaa cagcaaaatc gccatggtca tgcgccgcca catgcaccaa
     1801 tacggcacga ctttggatca cctcggcaag atcgccgtcg ccggccgtta tcatgcgagt
     1861 ttgaatccca gcgcttactt gagaaaaccg attacgattc aagattacaa agactcgcgc
     1921 ttggtcgccg atccggttcg gcttctcgac tgcgtcctgc cggcaaacgg cggcaaagcc
     1981 tacatcatgg cgtcgagcga acgagcgaaa agcctgcgca agccgccggt cttcgtgcgc
     2041 ggcttcggtg aaagaagcaa tccgtcctac ggcccgagag ccggctccga ctcactgatc
     2101 atgggtgtcg ccgatgccgg taaggtcgcg atggaaatgg ccggcgtaaa acacggcgat
     2161 atcaacttcc tcgaacttta tgatgactac atcattgtgg tttacttgca gatcgaagat
     2221 ctggggtttt gcgccaaggg cgatctcggg ttttttgaac gaaccgattt cacgatcaac
     2281 ggccaactac cgattcaaac cggcggtggc atgatcaact gcggccaacc ttctaccgcg
     2341 ggtggaacca ttcacgtcat cgaggcggtc cggcagttgc gcggcgaagg cggtcagcgc
     2401 caagtgcgcg acgccaaaat aggcatggtt accggcttgg gagttctgcc ttatggcaag
     2461 aatctcggct gctgcgcggt ggcggttttg gatagagaag catgattgaa aggcgcccgc
     2521 cccagttgca gatcgccgat gtaattaggt tcagaagctc cgagggtcct gacctctgcg
     2581 gcacggatga gagaaatgtt ggagcatctc taggctaaga acgatttcgc gctacccgtc
     2641 gaaatggcaa caagagcaag gtggcgacat tgggtcaggc catcgaaagg taattttctg
     2701 acgttgaagc tttgaacggt tgaactttga accaagctcc aggagcctct actacccatg
     2761 actgaaaccg acctgcaaaa accattacct acgatcacca gcgaagcgaa acctttttgg
     2821 gatggcgcag cgaagcagaa gctgatgatg cagcgctgcg tcgattgccg ggcctatgtc
     2881 tggacgccgc ggccgagctg ctttgaatgc ggcagcgaaa acattcaatg gcaggagctc
     2941 agcggaaaag gaaaggttta ttccttcacc gtgattcgcc aggtcgttgg ccgcgcggca
     3001 tcgcaggcgt ttgaaaaaga catcccctat gtgatcgcct ggatcgacct ggacgaaggc
     3061 ccgcggatga tcagcaatgt gatcggttgc ccggttgagg cggtgaagct gggcatgaaa
     3121 gtgtcggtca tcttcgagca gcaatcggct gatatatggt tgccgaaatt taggcctgtg
     3181 agctcgtaaa actaaacctc agaaaagcta ctcggccgag actcaattgt ttccgtcccg
     3241 ctcctttccc gcgaacttac ttttctcttt tcccaggtgc cgaccgaaga aggtaatcac
     3301 ctcgcgaatg ctgtcggccc acgccttggg atcgcctccg gtggtagcac cgcggccggt
     3361 gggggagttc gtgttaatgc gagccgccac gtgacgcacg gggttgctgc tgtcgaagga
     3421 gtggtgtgcc cccggataaa tcttaatcgc cacaggatac ccctcttgca cagccgcctc
     3481 cgtcagcttt cggcacggtt ccgccggcgt ccaatcgtcg ttttctccta taagaatcaa
     3541 caaaggagca atcgccttgt acgcaccgac atagctaagg gtgggcccgt tcatccccga
     3601 tcggctctca ggacgccagc ttcccaggcg gattgcacag ttcggataca gagcgacggc
     3661 ggccgcgaag cctgttcgtt tgtctcgagc cagtggctct ctatcgcttt ccggcgaaac
     3721 cattgaggct agagttgtgg aaccgccgtg cgagccgccc atgagtccaa ttcgggctct
     3781 gtcgacatac ggcagcgtgc gtagataaga gagtgcagca taagcatcgc gggtgcgccg
     3841 aaatgggctc acttcggtcc ggctgggaga cggattggtg catacgccac tggcgtgccc
     3901 tcgcgttgtg aagctatccg gaatgagcac gacgtatccg tacgtcagaa gttggtttgc
     3961 ccagcggtcc ggagcgccgc ttgagcgaga accgaggccg ctgcaatcgt gcataatgac
     4021 cactgctgga aacaccccgg cgccgtcggg cttcaagagc ttggccggaa tctgctccga
     4081 tgctccagtg ccattagtcg tgctaataga caagtccgaa gtggtggagc tgcatccggc
     4141 gagatacaac actaaaagca ccgacaatat cacatccacc agcatagctt tcgccaagaa
     4201 caattaagcc gaaagtcgtg cgtgtgcgac gtcgcatgcg cacgaacaga gtcacacttt
     4261 agagttagag tcccgtcgtg cttgccggcg ggcggcgatg atgcgttgcc tgttcgtacg
     4321 cgtacgcaaa tttgatcaag cttccgtcat catagggtcg cccggtaaag caaacaccag
     4381 ccggaagatt gtcccgcgta aatcccgcgg gcacgacgat cgtcggcacg aaaaccagaa
     4441 acgtatttaa atgcggcgcg cctttctgat cgacgaacgg cgggttcacg ccgtctttga
     4501 tcagagtagg ctggtgctct atcgccttgt gcacgatcgc atccagacga tgatccgcca
     4561 tgactttgag aaagttcgtc atcaactcat cttgcgcttt gagatgttcg taatggctca
     4621 tgggatcgg
//
LOCUS       DASUVS010000077         7767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1774492, whole genome shotgun sequence.
ACCESSION   DASUVS010000077 DASUVS010000000
VERSION     DASUVS010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774492"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VF182_04480"
     CDS             <1..316
                     /locus_tag="VF182_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436363.1"
                     /translation="ILGWRNPFYMRRNVTRARPRKREISQETLRSIEANNKLDLELYR
                     YAKKLFKEQIGSQSPSFENELQRFRKLNETCGRLHVLLSVGRRVTAGKILAGARLFMR
                     HE"
     gene            321..1247
                     /locus_tag="VF182_04485"
     CDS             321..1247
                     /locus_tag="VF182_04485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX6436364.1"
                     /translation="MIVSVVIIFLNAERFLQEAIESVFAQTYSAWELLLVDDGSTDGG
                     TTIAQRYARYHPGQVRYLEHPGHANRGMSASRNLGIRNARGSYVAFLDADDVWLSNIL
                     EEQVAILETYPEAAMVYGPIEYWYSWTGMPEDRERDYVEKLGVPPDTIIQPPRLFPLF
                     LRDKATVPSGILVRRGDIERVGGFEDTFRGEYEDQVFCAKICLDAPVFASGRSWYRYR
                     QHPGSCVLTGQTTGETHSARLLFLNWLTKYLSEQKVKDREIWRALSLEFWRFTHPRAF
                     RLLLRGERFINRINGLITQYRKPSGNRTCPNY"
     gene            1288..2013
                     /locus_tag="VF182_04490"
     CDS             1288..2013
                     /locus_tag="VF182_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6436365.1"
                     /translation="MTLPVFHWLRAKWKRLGYATPAGWVRFGSLRRVTPLSREFGYDR
                     GLPIDRYYIERFLAKNASDIRGYVMEVADNTYTLRFGGDWVTRSDVLHVEAGHPNATI
                     VADLTCADHIPSGNFDCVILTQTLQFIHDVPAVLRTVKRILKPGGVVLVTMPGISPIS
                     RYDMERWGQCWAFTTQSARRLFEGVFPQDHVQITAYGNVLTAASFLYGIATEELRKEE
                     MDYFDPDFELIIAVRAVKPANVS"
     gene            2081..2899
                     /locus_tag="VF182_04495"
     CDS             2081..2899
                     /locus_tag="VF182_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436366.1"
                     /translation="MFAVEIKSSVGLGAKLEWCLEILAYCHDNGLVPRFRFSYPNSEK
                     SEDYFGKFFKVKEIFVANKPVRFTTINSIVELNLGKDYDRILTIELATYLIRKYLTVQ
                     DDVLSEVESFCHQHFAGRRVVGVHYRGTDKGKESPIIPYDTVKRNIEHYLKLHPETNC
                     VFISSDDINFIEDMERAPVGRPIIYRNDSLRARDGNSIHESADTNKYEINRDAIVNCL
                     LLSRCDALLKTPSILSGWSKLFNPKLPVVVLNSPYEQWFPERDLIRENVFVPVL"
     gene            3027..3956
                     /locus_tag="VF182_04500"
     CDS             3027..3956
                     /locus_tag="VF182_04500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008186663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX6436367.1"
                     /translation="MSNKPLVSVVVIFFNGEAFLREAIESVFAQTYRHWELLLVDDGS
                     IDQSRSIAERYVEQYPDKVRCLEHEGQQNRGMSASRNLGIENAKGEYIAFLDGDDVWL
                     PPKLERQVSLLSAQPEVGMIYGAAQWWYSWTGKPEDRARDFVHPLGVMPDKCIEPPRL
                     LLQFLMNEGTSPCIGTVMARRAVLERTGGFEEKFRGMYEDQAFCAKICLATRVFVSSE
                     CWHPYRQHAGSASAVAHKTGRHRAARLFFLSWLESYLTEHDIKDLAVWQTLEKELWRC
                     QHPFLYRMKVRTRQLARVINAQLRGVIRTGDAR"
     gene            4281..5927
                     /locus_tag="VF182_04505"
     CDS             4281..5927
                     /locus_tag="VF182_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX6436368.1"
                     /translation="MLITYLGHAGFCVELEKSTIIMDPWLSPFGAFDSAWFQYPCNHH
                     LNDLVERKLQDATKDRYIYISHEHQDHFDLGFLNSLKTRDFKFIVPQFFRSESLAHYQ
                     CGGVILCHSDEEVRLADGFIKLFLYGHGFEHDSAILVHCDGQSFLNLNDCRVYDHLQE
                     IRKTNGDIDVVAGQFSGASWHPTCYDYPEQVYQRISQTKTISKFEAIARAIEAINAKI
                     FLPSAGPPCFLDPMLFHLNVEPINVFSRPPQLIKYLQERVGRSERQHTLEIMEMMPGD
                     ILDVTECNFAYKVQERPSEDGSDAYLRSYAARYEVFFKDREVECSSQHLDDIFHRLLE
                     AMEYKLSLFALSHLVTTALYLSLQETPDKLIRVDFQSRRVEVVSGVIDPNYYSLSTPT
                     WQMERVLDHKMTWYNFSLTFRMRIKREPDVYVPLLHDFLLTEAEDLKQFCSEWLQLET
                     KLEENGERITVEAGGCLYSVSRFCPHQGADLREGWIEENRYLVCPGHWWRFDLRNKGR
                     CEASNATINAVALEQDLPKPRVPTAQPAALRDCRSREVSR"
     gene            6167..6985
                     /locus_tag="VF182_04510"
     CDS             6167..6985
                     /locus_tag="VF182_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436369.1"
                     /translation="MRSLSHAARTVAWYLARWPVIRQLRCLQFRRLQPIGNGRQRGTP
                     IVRYYWEGYLQEHQADIRGTVLEIGTTDTIRRFGGIAVTCAEGIDLSPHSPEVTVVAD
                     LSRADHVPSDRYDCFVNQFTMHLIYDAEAALYHSIRLLKPGGILLINFSCVDYYFPSG
                     LDMGTGESLFLYRWYTPIQVENMLRNLGLRAEDYTIEIYGNLFTRIAYQMNMAAEELT
                     RRELEYADAGHPLLICARIAKPADWQADKPDYRTPWNPRVTPAQWNSVTGHYGT"
     gene            6997..>7767
                     /locus_tag="VF182_04515"
     CDS             6997..>7767
                     /locus_tag="VF182_04515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6436370.1"
                     /translation="MMIRAEPSTVSGTLSCYRTRSNPLQESVVHLRGWQLACAISVVL
                     FVAAVGIVLTTQGWRSRSPAHDLVPHIINAHNLATTGAIPIHGDTGSYGSYKPAGTAW
                     LMLPSTLLFSDPRLSEYIGAALLHLAALVGIFLLAYNYFGFWCACFAVIIYGLSSTGI
                     LMAASLWPNGRPDFFIWTVYFASRWATRKDGKSLAIATAIWGIGMNVDMAILPAIFIL
                     PALWLVYRPPIKFKPLIIAMGLVLLVWSPYLRLEIGRGF"
BASE COUNT         1887 a         1876 c         2014 g         1990 t
ORIGIN      
        1 aatcctcggc tggcgcaacc ccttttacat gagacggaat gtcacccgcg cccgtcctcg
       61 caagcgcgag atttcgcaag aaacattacg ttccatagag gccaataaca agctcgatct
      121 cgaactctat cgctatgcca aaaagctctt taaagagcag attggctctc agagtccgtc
      181 gttcgagaac gagcttcaac ggttcaggaa gctgaatgaa acctgcggca ggctgcacgt
      241 gctgctatca gttggcagga gagtaacggc ggggaagatc cttgccggcg cgcgcctatt
      301 catgaggcat gagtgatcgg atgattgtct ctgtcgtaat cattttcttg aatgcggagc
      361 gattccttca agaagcgatc gagagtgtct tcgcccaaac gtattctgcc tgggaactct
      421 tgctggtgga tgatggctcg accgacggag ggacgaccat cgctcaacgc tatgccagat
      481 atcatcctgg tcaagtgcgt tatctcgagc acccggggca tgccaatcgc gggatgagcg
      541 cgtcgcgtaa tctgggtatt cgtaacgcgc ggggatcata cgttgcgttc ctcgacgccg
      601 atgatgtctg gctttcgaac atcttggaag aacaggtcgc gatccttgag acttatccag
      661 aggccgccat ggtctatggg cccatcgaat attggtatag ctggacaggc atgcctgaag
      721 atcgcgaacg tgattatgtg gaaaaacttg gcgtgccgcc ggacaccatc attcaacccc
      781 ctcggctttt tcctcttttt ttacgtgaca aagcgaccgt cccatccggc atcctggtgc
      841 gacgtggcga tatcgagcgg gtaggtggat tcgaagacac ctttcgaggc gaatatgaag
      901 atcaggtctt ctgcgcgaag atctgcctcg acgcaccggt attcgcatcc ggtcgctctt
      961 ggtaccgtta ccgacagcac cccggttcgt gtgtgttgac gggtcaaaca acaggagaaa
     1021 ctcattctgc gcgcttgctt ttcttgaact ggcttaccaa gtatttgtct gaacaaaaag
     1081 tcaaagaccg cgaaatttgg cgcgcgcttt cgttagagtt ctggcgtttc acccacccgc
     1141 gcgcttttcg cctgctactt cgcggcgagc gttttataaa tcgaatcaat ggattgatca
     1201 cgcaatatcg gaaacctagt ggaaaccgta cttgtcccaa ctattgagag atcgcggcat
     1261 cttatactcg cgatagccca gcgtacattg acccttccgg tttttcactg gcttcgggcg
     1321 aaatggaaaa gacttggcta tgctacgccc gccgggtggg tacgctttgg cagcttgcga
     1381 cgcgtcacac ccctcagccg cgagttcggg tatgatcgcg gtttgccgat tgatcggtat
     1441 tatatcgagc gcttcctggc gaagaacgcg tccgacatcc gcgggtatgt catggaagtt
     1501 gccgacaaca cctatacgct ccggtttggc ggcgactggg tgacccggag cgatgtgctt
     1561 cacgttgaag caggtcatcc gaatgcaacc attgtggcgg acttgacctg tgccgaccat
     1621 attccgtccg ggaatttcga ctgtgtgatt ctcacgcaga cgctccaatt tatccacgat
     1681 gtgccagcgg tgctgcgcac ggtaaagcga atattgaagc ccggaggagt ggtgctggtc
     1741 accatgcccg gaatcagccc aattagccgc tacgacatgg agaggtgggg ccaatgctgg
     1801 gcgtttacga ctcagtctgc acgacgacta ttcgagggag tttttcctca agaccatgtc
     1861 cagataacgg cttatggcaa cgtgctcacg gcggcatcgt ttctgtatgg catcgcgacc
     1921 gaagagctac gaaaggaaga gatggattat tttgatccgg attttgagct catcatcgcc
     1981 gttagagcgg taaaacctgc gaatgtttct taggcggatt aaaacaagag taaagcgttc
     2041 ccttatttca ctgttggcca aatacaactc cagaaatggg gtgtttgccg tcgagataaa
     2101 gagttccgtt ggtttaggag cgaaattgga gtggtgttta gagattctgg catactgtca
     2161 tgacaatggt cttgttcctc gattcagatt ttcttatccg aattccgaaa agtccgagga
     2221 ttatttcggt aaatttttca aggtgaaaga gatatttgtt gcaaacaagc cggtgagatt
     2281 tactacgatt aattctatag tagaacttaa tttaggcaaa gattacgata ggatcctgac
     2341 gattgagttg gcgacttacc tgattcgtaa gtacctcact gtgcaggacg atgtcttaag
     2401 cgaagttgag agtttctgtc atcaacattt tgctggcaga agagtcgtag gggtgcatta
     2461 ccgaggaacg gataagggaa aggaatcacc gatcattcca tatgataccg taaagagaaa
     2521 tatcgagcac tatttaaagc tacatccgga aaccaactgt gtatttattt cgagtgacga
     2581 cataaatttc attgaagata tggaaagggc gcctgtcggt cgaccgataa tctaccgaaa
     2641 tgattcattg cgcgcacgag atggtaactc tattcatgaa tccgcagata caaataaata
     2701 cgagataaat agggatgcaa tcgttaattg ccttctttta tccagatgtg acgctttgtt
     2761 aaaaacaccg tcgatcctaa gcggttggtc aaaactcttt aaccccaagc ttcccgtggt
     2821 tgtccttaac tctccttacg aacaatggtt tccggaaagg gatttgattc gggagaatgt
     2881 atttgtaccc gttctataat cacccgggcg gacaccattc gagtattcca acggctccgc
     2941 tgccggacca cagctagccg cgcgcctagt catgcaaatg atcgacagct gataggcaat
     3001 tggctgttca gctgtagatg acgtccatga gcaataaacc actcgtctcg gtcgttgtaa
     3061 ttttttttaa tggggaagcg tttctccggg aagcgattga aagtgttttt gcgcagacct
     3121 atcggcactg ggaactatta cttgtggatg acggctctat cgatcaaagt cgtagcatcg
     3181 ctgaacgtta tgttgagcaa tatcctgaca aagtacgatg ccttgagcac gagggccaac
     3241 agaatcgcgg aatgagcgca tcgcgcaacc tcggcatcga aaacgcgaaa ggtgagtaca
     3301 tcgcatttct agatggcgat gacgtctggt tgccgccaaa attggagcgg caagtgtcgc
     3361 tcttgagtgc tcagcccgag gtaggaatga tctacggcgc agcgcagtgg tggtatagct
     3421 ggacggggaa accagaagac cgcgcacgcg actttgtcca cccgctgggc gtaatgcctg
     3481 ataagtgcat cgaaccaccg cgtttactgc tccagttctt gatgaatgag ggaacttcgc
     3541 cttgcatcgg cactgttatg gcccgccgag ccgttctaga gcggacgggt ggatttgaag
     3601 aaaaatttcg ggggatgtat gaggatcaag ctttttgcgc caaaatttgc ctcgctacgc
     3661 gtgtgtttgt ctcaagcgag tgttggcatc cttaccgcca gcacgcagga tcagcgagtg
     3721 cggttgcaca taaaacagga cggcatcgtg cagcgcgact gtttttctta agttggttgg
     3781 aatcttactt aacggaacac gacatcaaag acttggctgt ctggcaaact ctcgaaaagg
     3841 agttatggcg atgccagcat ccattcttgt accgcatgaa ggtgcgaacg cggcaattag
     3901 cgcgagtcat caacgcgcaa ctgcgaggag ttatccggac gggcgacgcc agataaacta
     3961 cacagagtga ttgtcggctc tttgtggtga gcccggcgac tccacgaggt gacgcggagc
     4021 cgcgaagcgg tacattacga cccgcagcga cagtctatag cctgcctcag tcccgcctaa
     4081 catcttaacc gcgtattcca agcgatctat cacagccagg cgggacctac ggcaaagagt
     4141 ggcgctgatg ttatccgaca tgaccgcgta actgtgagca ctcttcgacg caacatagct
     4201 ggtgatatgc gcacttcaac cattagctgc tgctatgggc ctggtgacaa actaaaaatc
     4261 ttggacactt tcgcataacg atgttaatca cctacttagg acatgccggt ttttgtgtcg
     4321 agctcgaaaa gtcaacgatc atcatggatc cttggttgtc gcctttcgga gcttttgatt
     4381 cggcatggtt tcaataccca tgtaatcatc atctcaatga ccttgtcgag cgaaaactcc
     4441 aagacgcgac aaaggatcgt tatatctata taagccatga acatcaggat cactttgatt
     4501 tgggattctt gaatagcctg aaaacaagag attttaagtt cattgtcccg caattttttc
     4561 gctccgaatc actagcacac tatcaatgtg gaggtgtgat tctctgccat tctgatgagg
     4621 aggtaaggct tgccgacgga tttatcaaat tatttttata cggtcatgga tttgaacatg
     4681 actccgctat tctcgttcat tgtgacggac agagtttcct taatctgaac gactgccggg
     4741 tttatgacca ccttcaagag atcagaaaaa ccaacggaga catagatgtt gttgcgggtc
     4801 aattttctgg agcgagctgg catccaacct gttacgatta cccggaacag gtatatcaga
     4861 ggatttcgca gacgaaaacg ataagcaagt tcgaagcaat agcgagagcc attgaagcca
     4921 taaatgcaaa aatatttttg ccctcagcag gtcccccctg ttttttggat ccaatgctat
     4981 ttcatttgaa cgtggagcca ataaatgtat tttcgcggcc gccccaattg attaaatatc
     5041 tgcaggaaag ggtcggccga tctgagcggc agcatacgct ggaaatcatg gaaatgatgc
     5101 cgggagatat cttggatgtg actgagtgca attttgctta taaggtacag gagaggccca
     5161 gcgaggatgg cagtgacgcg tatctcagat cgtatgcagc tagatatgaa gtatttttta
     5221 aggaccgcga ggtagagtgt tcatcacaac acttggatga tattttccat cgtcttttag
     5281 aagcgatgga atataaattg tctttgtttg cgttgagtca tctggtcact accgctcttt
     5341 atctatcttt acaggagacg ccagataagc tgatccgggt agactttcaa tcccgtagag
     5401 tagaagtagt gtctggagtt attgacccca attactattc tttgtctaca ccgacgtggc
     5461 aaatggagcg cgtattagac cataaaatga catggtacaa tttttcgctt acttttagga
     5521 tgcgtataaa gcgcgaaccg gatgtttatg tgccattgct ccatgatttt ttactcacgg
     5581 aggcggaaga tctaaagcaa ttctgctcgg aatggctgca gttggaaaca aaactagaag
     5641 aaaacggcga acgcatcact gtagaagcgg ggggttgcct ttactctgta tcgagatttt
     5701 gccctcatca aggtgcggat ttgagagaag gatggataga agaaaatcgt tacctggttt
     5761 gtcccgggca ttggtggcgc tttgatctgc gaaataaggg tcgatgtgag gcaagcaacg
     5821 cgacgataaa tgcggttgct cttgaacaag atctacctaa gcctcgtgtc ccaacagctc
     5881 agcctgctgc tctgcgtgac tgtagatctc gcgaagtgtc ccgctaggag agttgccggg
     5941 tacgctaata catcccgttt atagacttcc ggtatttcgc cctccagccg cacatgccta
     6001 ctgcggcaca gattctggaa catacgagcg gatttataga ggaagttctg tatgctgtac
     6061 gaggaccaat ccctcacgcc aaaatttgtt tcccctaagc cggcgatcac agcgttttca
     6121 ttcaaatcac acggggagaa cgctttacga agcgagggtc ataccattgc gtagtttatc
     6181 tcatgccgca cgcactgtgg cctggtactt ggctcgttgg cccgttattc gtcaattgcg
     6241 ctgccttcaa tttcgacggc ttcagccgat tggcaatggc cgacaacgtg gtaccccgat
     6301 cgttcggtac tattgggaag ggtatctcca agagcatcag gcagatattc gtgggaccgt
     6361 tctagagatc ggcaccactg atacgattcg ccggttcgga gggatcgcag tcacatgcgc
     6421 tgagggcatc gacctgtcgc cgcatagccc ggaggtcaca gtagtcgccg acctcagccg
     6481 cgcagatcat gtgccgtcgg accgctacga ttgctttgtc aaccaattta caatgcatct
     6541 catctacgat gctgaagccg ctctttacca ctcgatccgc cttttaaagc cgggtggtat
     6601 cttactcata aacttctcgt gcgtggatta ctatttccca agtggtcttg atatgggtac
     6661 gggggagtcg ctcttcctgt atcgatggta tacgcccatt caggtcgaaa atatgctccg
     6721 aaatcttggc ctgcgagcag aggattatac cattgagata tacggcaatc tatttacccg
     6781 catcgcctat cagatgaata tggcggcgga agagcttacc cggcgtgaac tggaatacgc
     6841 agatgcgggc catccattac tgatctgcgc gcgaatagcg aaaccggccg actggcaagc
     6901 ggacaagccc gattatagaa caccctggaa tcccagggtg acgccggctc aatggaattc
     6961 cgtgacagga cactacggta cctgagtaag gcaactatga tgataagagc ggaaccctcg
     7021 accgtctccg gcactttatc ttgctaccgc actcgcagta acccgcttca ggaatcagtc
     7081 gtgcatctca ggggttggca gttagcgtgc gcgatttcgg tcgtgctttt cgtcgcggcc
     7141 gtgggaattg tgcttacgac tcagggctgg cgatcgcgca gtcccgcgca tgatctggtt
     7201 ccccacatca ttaatgcgca caatctcgcg acaacagggg cgatccctat acatggcgac
     7261 acaggaagct atggttctta caaaccggcg gggactgcct ggctcatgtt accaagcacg
     7321 ctccttttct cggatccacg gctttctgaa tatatcggcg cagccttact tcacctggcc
     7381 gcattggtgg ggatctttct cctggcctac aattattttg gcttctggtg tgcctgtttc
     7441 gccgtcataa tttacgggct atccagcaca ggaatattga tggctgcctc tctttggccg
     7501 aacgggagac ctgacttttt tatctggact gtctatttcg caagcagatg ggcaacccgc
     7561 aaagatggta aatccctagc tatagcgaca gccatctggg gaattgggat gaacgtggac
     7621 atggcgattc tacccgccat tttcattctc ccagctttgt ggttggtata tcgccctccg
     7681 attaaattca agccattaat cattgcaatg ggtcttgttt tgttggtttg gtcgccttac
     7741 ctccgacttg agatcgggcg cgggttt
//
LOCUS       DASUVS010000078         4079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_901769, whole genome shotgun sequence.
ACCESSION   DASUVS010000078 DASUVS010000000
VERSION     DASUVS010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901769"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VF182_04520"
     CDS             <1..451
                     /locus_tag="VF182_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248026.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HEX6436371.1"
                     /translation="KETLVSLAKLVDEGEKYGIPVLAVTAVGKDMARDVRYLGLACRI
                     AAELGAHIVKTYYCDDFEQLIESCPIPVIVAGGKKTPELEALELTYNSVKGGAVGVDM
                     GRNIFQSDCPVGMIQAVRAIVHENVSVKEACKIYESAKGAIAKRAAG"
     gene            618..1631
                     /locus_tag="VF182_04525"
     CDS             618..1631
                     /locus_tag="VF182_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX6436372.1"
                     /translation="MYYNNQDVRLEEMRVPKIGAGELLLCIRASGICGSDLMEWYRIK
                     KAPLVLGHEITGQVVEVGDGVRKFRVGDRVFSSHHVPCGNCRYCHAGHQSVCEMLRST
                     HFEPGGFSEFVRVPAINVDLGTFLLPEEVTFDEGSFIEPLACVVRAQRFARLAAGQTV
                     LVIGSGISGLLHIQLARARGAQRIIATDISDFRLKAAAKFGAEATIHGAEQVPARLRE
                     LNEGRLADLVIVCTAAMPAIQQAVKSVDRGGTLLFFAPTAAGVDVPIPLFDFWRDEIS
                     IVTSYAGSGGDLVESLELIRTHQVRVGEMITHRLPLSQAGLGFQLTASGSESIKVILD
                     PSI"
     gene            complement(1648..2646)
                     /locus_tag="VF182_04530"
     CDS             complement(1648..2646)
                     /locus_tag="VF182_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436373.1"
                     /translation="MRLFQHLRILLMFAAAVGAVHFPSAGHAQSQLKKVRMGSSSTNV
                     SFLAIYTALHRGFFKDEGIDLEIIFMPANLASTAVLNGDIDYNGAVTGTIGAAVRDQP
                     MKVLLFTVSKPLLFLVSQKNIKDVKQLKGKKIAGSSPGGSATLLANQALKQIGLEPGK
                     DVSVLQMSGNAASRFAVLESGVVDASLLSVPENIVALEKGYNELLFLGDIVEFAQNGF
                     GTSNKRIRENPDEVYRMVRATVRGLQFIWDKNNNDAVTNILMKQWNISDRKMAGEMFR
                     HVSRVLTKDAYVRPESVQVLIDLARENAKVTKPIPASEVVDYSFLNKARKELGITK"
     gene            complement(2671..>4079)
                     /locus_tag="VF182_04535"
     CDS             complement(2671..>4079)
                     /locus_tag="VF182_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX6436374.1"
                     /translation="DRLREECGHQDARVGCIGPAGESLNRAAMFVNDYNHNAAHGLGA
                     VMGSKKLKAIVAWGTKRPRVHDKNTLIEAGLRWRKAIASRTTTVKKRLTSVGHGEDWG
                     AITKLNWRSTVISDEAKGLDQNQVVLRPCFQCARMCPWDVKIGEGRFKGAVGHFNAGS
                     EWMDTFYNLGFKGNDVLYLSERINDLGIECSHFACGAGLAFEAWEKGVLGEDRTDGLR
                     LEWGNLHAAETLLERCARRETWLGNILADGPKELAEALGGEAKNWVVHTKGGTPAQHE
                     WRPLLSQMLRELVASGGMKPQGAGGTEPPADLLYREKWGPLDPTQADGWTNSHLLTEQ
                     IRQACGVMGGCWFALNDKPPDGVKSMVDSLNATTGWNVSLDETIDVGHRSIILQSVFG
                     TQRGWIADHDWQDVGPRFLEPIPDGKYKGFTIAKWLPELIFEYYRLSGRHERTGRPYI
                     DTLTRLGLEEFKQWSQLD"
BASE COUNT          836 a         1110 c         1165 g          968 t
ORIGIN      
        1 gaaggagaca ctggtgagcc tggccaaact ggtcgatgag ggggagaagt acggcatccc
       61 agtgctcgca gtgacagcgg tgggcaagga catggcgcgt gacgttcgct acttagggtt
      121 ggcctgccgt attgccgcgg agcttggcgc gcacatcgtt aaaacctatt actgtgacga
      181 cttcgagcag ttgatcgaga gctgcccaat tccagtgatc gtggcgggcg gcaagaaaac
      241 gccagagctc gaggccttgg aattgaccta caattcagtc aaaggcggtg ctgtgggagt
      301 cgatatggga cgaaatattt ttcagtccga ttgtccggtg gggatgattc aagcggtgcg
      361 ggcgatcgta catgagaatg tctcggtgaa agaagcgtgt aagatttatg agagcgctaa
      421 gggagcgatc gcaaagcggg cagccggatg agcgagttgc ggagatgcag acgtgaacga
      481 ggccaatgtt gagcggcaat ctcaagcgtc cggcgtaggc tcaggaagat ccagtattct
      541 gtctggtagt cgatctctcc tgagagatgc atttgcatac ttctaagcag atagatcctc
      601 gcagcatgcg ggtggcgatg tattacaaca accaggacgt gcggcttgaa gagatgcgcg
      661 ttccgaagat cggagccggt gagttgctgc tgtgcattcg cgccagcggg atttgcggca
      721 gcgatctgat ggaatggtac cgcatcaaaa aagcgccgct cgtcttgggc cacgagatta
      781 ccggccaagt cgtcgaagtc ggcgacggcg ttagaaaatt ccgcgtcggc gaccgagtct
      841 tttcctcgca tcatgtgccg tgcggaaact gccggtattg tcatgccggt caccagtcgg
      901 tttgcgaaat gctccgcagc acgcattttg agccgggcgg cttctctgaa ttcgtccgcg
      961 tgccagccat caatgtagat ctcggaacgt ttcttttacc ggaggaagta accttcgatg
     1021 aaggttcctt tatcgaaccg cttgcatgcg tggttcgagc ccagcgcttt gctcgtcttg
     1081 cagccggcca gactgtgctc gtcatcggca gcggaatctc gggtttattg cacatccaac
     1141 tggcgcgtgc tcgcggcgct cagcgaatca tagccaccga catcagtgat ttccgtttga
     1201 aggcggcggc taagttcgga gccgaggcaa cgattcacgg cgccgagcag gttccggcgc
     1261 gattacgtga gctgaacgaa ggccggttgg ctgatctggt gattgtctgc acggccgcga
     1321 tgccggccat tcaacaggcg gtaaaatcgg tcgatcgggg cggcacgctg ctgttctttg
     1381 cgccgaccgc agccggagtc gatgtgccga taccgttgtt tgatttttgg cgagatgaga
     1441 tttccatcgt cacttcgtac gccggcagcg ggggtgattt ggttgaatcg ctcgaactta
     1501 tccgcacgca tcaagtgcgg gttggagaga tgatcacgca tcgcttgccg ttatcgcagg
     1561 cgggtttagg atttcaactt acggccagcg gctcggaatc gatcaaagta attctcgatc
     1621 cttcgatcta gtagtgtgtg cctccggtca cttcgtgatc cccagctctt tgcgagcttt
     1681 gtttagaaaa gaataatcga cgacttcgga agccgggatc ggctttgtta ccttggcgtt
     1741 ttctcgggct aggtcaatga gcacttgaac ggactccggc cggacatagg cgtctttggt
     1801 taacacccgg ctcacgtgcc gaaacatttc tcccgccatt tttctatcgc tgatattcca
     1861 ttgcttcatc aaaatgttgg tcactgcatc gttattattt ttgtcccaaa taaattgtag
     1921 gccgcggact gtggcgcgca ccattcgata aacctcgtcg ggattctcgc ggatacgctt
     1981 gtttgaagtg ccgaagccgt tttgggcaaa ctcgacaatg tcgccaagga agagcagttc
     2041 gttgtagcct ttctccaatg ccacgatgtt ctcaggcacc gaaaggagcg aggcatcgac
     2101 gacaccggac tcgagaacgg cgaagcggct ggcggcgttc cccgacatct gcaaaactga
     2161 aacgtcctta cccggctcga gtccgatctg tttcagcgct tggttggcga gaagtgtcgc
     2221 cgagcctccc ggtgagctgc cggctatttt tttgcccttt aactgtttga cgtctttgat
     2281 atttttttgg ctgacgagga acaacagtgg cttggagacc gtgaagagca ataccttcat
     2341 cggctgatcg cgaactgctg caccgatggt gccggtcaca gcgccgttgt aatcgatatc
     2401 gccgttcaaa actgcggtgc ttgccaggtt ggcgggcatg aagatgattt ccagatcgat
     2461 cccttcatcc ttaaagaatc cacggtgaag cgccgtataa atagccagga aggagacatt
     2521 ggtggaagaa ctgcccatgc gcactttctt taactgagac tgggcatggc cagcggaagg
     2581 gaagtgaaca gcacccaccg ccgcggcgaa cattaaaagg attctcaggt gttggaaaag
     2641 tctcatagcg gcatccttgc ttcgtcgatc tcaatccagt tggctccatt gtttgaactc
     2701 ttcaagtccc aagcgggtta gcgtgtcgat atagggccgc ccggttcttt catgccgccc
     2761 ggatagacgg tagtactcga aaatgagctc gggcaaccat ttcgcaatgg tgaagccctt
     2821 gtacttgccg tcagggatcg gctcgagaaa gcgcggtccc acatcttgcc aatcatggtc
     2881 ggcaatccac ccgcgctggg tgccgaaaac gctttgaaga atgatgctcc ggtgaccgac
     2941 gtcgatggtt tcgtcgaggc tgacgttcca ccctgtggtt gcattaagcg agtccaccat
     3001 acttttcacg ccgtcgggcg gtttgtcgtt caaggcgaac cagcaaccac ccatgactcc
     3061 acaggcctgg cgaatttgct ccgttagaag atgcgaattg gtccagccat cggcctgagt
     3121 cggatcgagc ggaccccatt tctctcgata taacagatca gcgggcggct ctgtaccgcc
     3181 cgccccctgc ggtttcatgc cgccgcttgc cactagttcg cgcaacattt ggctgagcag
     3241 cggccgccat tcatgttgcg ccggcgtccc gccctttgta tgtactaccc aattttttgc
     3301 ttcgcctccc agtgcttcgg caagttcttt agggccatcg gcgagaatat tgccgagcca
     3361 tgtttcgcgt cgcgcgcagc gttccagcag agtttcggca gcgtgcaaat tgccccattc
     3421 caatctcaat ccgtcggtac gatcctcgcc aagaacgccc ttttcccaag cctcgaaggc
     3481 tagtccggcg ccgcaggcga aatggctgca ctcgatgccc aaatcgttga tgcgctccga
     3541 caagtagagc acatcattgc ctttgaaccc gagattgtag aaggtgtcca tccattcgct
     3601 gcccgcgttg aagtggccga ctgcgccttt gaagcggcct tcgccgattt tcacatccca
     3661 ggggcacatg cgcgcgcact gaaagcaggg cctcaagacg acctggttct gatccaaacc
     3721 cttggcctca tcgctgataa ccgtactgcg ccaattaagt ttcgtaatcg cgccccagtc
     3781 ctcgccgtgg ccgacactgg tcagtcgttt ttttactgtg gttgtgcgcg acgcgatggc
     3841 tttgcgccag cgcagtccgg cttcgatcag cgtattcttg tcgtgcaccc tgggccgctt
     3901 ggtgccccat gcgacaatgg ccttcagttt ttttgagccc atcacagcgc ccaaaccatg
     3961 ggccgcgtta tgattgtagt cattgacgaa catagcggca cgattcaggc tttcgccggc
     4021 cggtccgata caaccgacgc gcgcgtcctg atgaccgcat tcctcgcgga gcctgtctt
//
LOCUS       DASUVS010000079         9735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_465203, whole genome shotgun sequence.
ACCESSION   DASUVS010000079 DASUVS010000000
VERSION     DASUVS010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9735
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465203"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VF182_04540"
     CDS             complement(<1..203)
                     /locus_tag="VF182_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6436375.1"
                     /translation="MTKPIIKTVTRSDADRAVANVVLAFSADPIARWFYPDLHQYLLY
                     LPSFVQAFAGRSFEHNSAYSVDG"
     gene            complement(374..1756)
                     /locus_tag="VF182_04545"
     CDS             complement(374..1756)
                     /locus_tag="VF182_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008956089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6436376.1"
                     /translation="MNVQAANAREQLKGIVKGSVLIPSDSRYEEARQIWNAMIDRRPA
                     LIVQCAEADDVAHAIAFARNNKLEISIRGGGHNIAGNALCDGALTIDFSNMKNVRVDT
                     AKKRAYVEPGATLADLDQATQVHGLATPVGINSTTGIAGLTLGGGFGWLTRKYGMTID
                     NLVSVDMITADGNKIRASEKENSDLFWALRGGGGNFGVVTQFEFQLFPVGPEILAGLI
                     VFPFSQARQILTSYRQFIDSAPEDLNVWVVLRQAPPLPFLPENVHGKEVVVLPIFYCG
                     AVAEGEKLIDPLRAFGDVLGEHIGALPYADWQKAFDPLLTPGARNYWKSHNFTKLADG
                     ALNAVIEFAGSLPSPQCEIFIGLIAGAPNRIAANATAYPARDANFVLNVHGRWDKAKD
                     DQKCIAWARDFFKASAPYASGGAYINFMTDEEGDRVAAAYGSNYERLVEIKRRYDPEN
                     IFHLNQNIKP"
     gene            complement(1789..2700)
                     /locus_tag="VF182_04550"
     CDS             complement(1789..2700)
                     /locus_tag="VF182_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436377.1"
                     /translation="MQNHYSYQSTLAASERIGWRVEDLIGGAKKLDFTKPFMPESLAQ
                     VKQLSFLAPEEQVTLNQIRGHEYLLMFGLVEEFILPYVVDHARPQLSGDDYRVRALLQ
                     FAGEEAKHIHLFKRFRQEFEEGFGSKCDFIGPAEDISRFVLSHSPLGTGIAILHIEWI
                     TLRHYIEGVRNSQELDPQFKSLLKHHWLEESQHTKLDTLMVEEIVANSTAQEIDQAFA
                     DYANIGAFLDRGIQQQAEFDVESFLQATGRDLSRTEREQVKNAVLKGMRWTYLGTGMT
                     HPNFLATVETINPGARKQIEEMAPAFC"
     gene            complement(2990..4942)
                     /locus_tag="VF182_04555"
     CDS             complement(2990..4942)
                     /locus_tag="VF182_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6436378.1"
                     /translation="MIHHDPGVSPLAALDEESALRTILEGTANETGERFFAALVENLA
                     RALNTHGAWVTEYIQERRRLRALAFWMDGQWIKDYETDIAGTPCERVIDSADIVHFPD
                     NLFKLFPDDPDITAINAVSYLGMPLKDVDGKILGHLAVIDRRPIPKEPKVHALFRIFA
                     ARAAAELQRQSVERQVKEREEKLRRLFDSAMDAIIELDQNLKVTRVNPAAEQAFQCPA
                     GQAIGDEFSRFVSSKSYQKILRLIDEFKPCPPERRSAWVPGGLRALVSGGSEFAAEAT
                     LSCFEIGRQTFYTLILRNISERLEADRKIGSLTAEAEYLREALNKLGGFEQIIGQSAA
                     LKTVLEDIEQVAVGDTTVLILGETGTGKELIARAIHGASRRADKPLITINCAAIPAAL
                     MESEFFGHEKGAFTGATQRREGCFALADSGTIFLDEVGELSLDLQAKLLRVLQEGEFT
                     PVGGSLLRKVNVRVIAATNRDLKKAIREGHFREDLYYRLNVFPIGVPPLRDRNEDVVI
                     LASEFAAKFAQRMGRRIEPLSEPIKKRLKAYSWPGNVRELQNVIERAVITSRDGSINL
                     DRALPEIPLEVVIETVPSLDSAIHPAQRILPIHELQQLERENILRALESSGWRVAGKD
                     GAAGMLGINPSTLNSRIRALGIQRPR"
     gene            complement(5127..6080)
                     /locus_tag="VF182_04560"
     CDS             complement(5127..6080)
                     /locus_tag="VF182_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6436379.1"
                     /translation="MDRTRRMTNSAVANDQDVGLSGPSHILAPMSLLAEITAIFSAMG
                     WASDSVLIRLGLRRSNIFAAMAMSFTVSTACMWSYLIASTSLEFLSSPAMIYFVISGC
                     IQPLLARALFYEGITRIGVARAGPLRGTEPMFATIIGVSFLHEEPGVLVYIGTILIMT
                     SLWLISGRQDGAAKWRLVDTAFPISAALVSAISQSLRKQGLKILPDPFVAVAIVTTVS
                     LILLLVFVLSTGRAQSLRLDRQGFSFFLAAALLATTAQVLNFIALGRGELSVIIPLLN
                     TTPLFTVLLSVLFLRKVETVNRRIVFGALLMFAGIVTITSR"
     gene            6162..6611
                     /locus_tag="VF182_04565"
     CDS             6162..6611
                     /locus_tag="VF182_04565"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6436380.1"
                     /translation="MENGKQTEAKMVKLRTQLVKEGHTRNLLAETDLMTLRIHCYAPG
                     IGENALHSHTKEDHIFLVLEGNAEFNTGRDGKTILNASKHQAIVLPAGCYYQFRNSGD
                     TPLVMTRIGAGPDKQDQRLNPDGTPIPGRTHEQGAAKPVLIEGAFFE"
     gene            complement(7160..8137)
                     /locus_tag="VF182_04570"
     CDS             complement(7160..8137)
                     /locus_tag="VF182_04570"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX6436381.1"
                     /translation="MPEQTFLEAIRAGLHEEMSRDSAVYIFGEDVSLGGPFGVTKGLA
                     QDFGTQRVVDTPISEGTVMGLAIGAATAGLRPVVEIMFIDFITLALDQLVNHAAKLHY
                     MSGGQLAIPLTVRVQCGISGAMGAHHSQSLESWLAHVPGLKVVMPSNPADAKGLLKAA
                     IRDDNPVVFIEHRGLYRGLGNVPDGDHLVPIGKAAVLHTGQQVTIVALANMVGAALTA
                     ADELAASSISAEVIDPRSVSPLDIGTIVESVRKTGRMVVAHEAVEQGGIGAEITAAVQ
                     QQALYYLDAPILRVAAPFAPVPASPALEKEFLPGKKRIVESVKKLVSHS"
     gene            complement(8245..9216)
                     /locus_tag="VF182_04575"
     CDS             complement(8245..9216)
                     /locus_tag="VF182_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX6436382.1"
                     /translation="MASKQESSKDFVALLRRMCLIRAFEEKVSALYAERQIVGLLHLG
                     IGQEAVAVGVCSLLRQDDFVFGGHRSHGHALAKGADINRLMAEIAGRASGYCGGKGGS
                     MHIVALDAGFITATGVVGGTIPLALGAAFAAKEKKQGQVAVVFFGDGAGQAGPFHESL
                     NIASLWQLPVIFVCENNGYAEFTPLSAHTKIERLAQHAKTYGIASSTVDGNDLLAVRL
                     AMSKALAIARARKGPVFIECLTHRLRGHYEGDPAKYRELSQLSEWKKKDPIARFARLL
                     KSKKTITDEGFQAFESEARARVEEAAEFSLSSSWPAMEEVATQVTTL"
BASE COUNT         2239 a         2696 c         2631 g         2169 t
ORIGIN      
        1 tagccgtcaa cagaataggc gctattgtgt tcaaaggatc tgccggcgaa tgcctgcacg
       61 aagctcggta aatagagcaa gtattgatgc aaatcggggt agaaccaacg ggctatggga
      121 tccgcgctga aagcaagcac aacattggcg acggcccgat ccgcgtctga tcgcgttacc
      181 gttttgatga tgggtttagt catgttcttg tttccttact taattccagt tcattcgcgc
      241 cggatccgtc attaaactgg ttccgcgctc ttctcgactc gaagaacctc tcccgcctac
      301 gagtggccat aagcgatggc cacatagaca agcaccaggg cgaagagtaa aaagccttcc
      361 atggcgcatc gcttcaaggt ttgatgttct ggttcaaatg aaaaatattt tcgggatcgt
      421 agcgtctttt gatctccacc aggcgctcat aattggatcc gtaagccgcc gcaacgcgat
      481 cgccctcctc gtcggtcata aaatttatgt aagcgccgcc cgatgcataa ggtgcactgg
      541 ctttaaagaa atcgcgcgcc cacgcaatac atttctggtc gtctttcgct ttgtcccagc
      601 gcccatgcac gttcagcaca aagttcgcgt ccctggcagg ataggccgtc gcgttagcgg
      661 cgatccggtt cggcgcgccg gcaatcagcc cgataaaaat ttcgcactgc ggcgagggca
      721 ggctcccggc gaactcgata acggcattca acgctccatc ggcaagcttc gtaaagttat
      781 gggacttcca gtagtttctt gcaccggggg taagcaaagg atcgaacgct ttctgccagt
      841 cggcgtaagg cagagccccg atgtgctcgc cgaggacatc gccgaacgcg cgcagaggat
      901 cgatgagttt ctctccttct gctaccgcac cgcagtagaa gataggcaat acgaccactt
      961 ctttgccgtg aacgttttcc ggaagaaaag gcaatggcgg agcctggcgc agcacgaccc
     1021 agacattgag atcctcggga gccgaatcta taaattgacg ataactagtg agtatctgtc
     1081 ttgcctggct gaacgggaaa acgatcaacc ccgcgagtat ttcgggaccc accggaaaga
     1141 gttggaattc gaactgggtg accacgccaa aatttccgcc gccgcctcga agcgcccaga
     1201 aaagatccga gttttctttt tcacttgcgc gtattttgtt gccatccgct gttatcatgt
     1261 cgacggagac gagattgtcg atggtcatgc cgtatttgcg agtcagccag ccgaagccgc
     1321 cgcccagagt tagtccggca atgcccgttg tggaattaat ccccaccggc gtggccagac
     1381 catgcacctg ggtggcctga tctagatccg caagcgtcgc cccaggctcg acatacgcac
     1441 gcttcttagc ggtgtctacc cggacgttct tcatgttcga aaagtcgatc gtcagggcgc
     1501 cgtcacaaag ggcgttgccg gcgatattat gcccgccccc gcgaattgaa atttccagtt
     1561 tgttgtttcg ggcaaatgcg attgcgtgtg caacgtcgtc agcctcggcg cactgcacga
     1621 tcagcgccgg ccggcgatcg atcatcgcat tccagatctg acgagcttcc tcataacggg
     1681 aatcgctcgg aatcaggacg ctccctttga caataccttt gagctgttct ctcgcgtttg
     1741 ccgcttggac gttcataaca ttgatctcct tcttggatct tgttgccgtc agcaaaaagc
     1801 cggcgccatc tcctcaatct gtttgcgtgc cccggggttg atcgtctcga ctgtggcaag
     1861 gaagttcgga tgcgtcatgc cggtcccgag ataagtccag cgcattccct taagcacagc
     1921 attcttgacc tgctcgcgct cggtccggga cagatctcta ccggtcgctt gcaagaaact
     1981 ctcgacgtcg aactcggcct gctgctgaat gcctctatcg aggaatgcgc cgatattggc
     2041 gtagtcggca aaagcttggt caatttcctg ggcagtggaa ttggcgacga tttcctcaac
     2101 catcagggta tcgagcttgg tgtgttggga ttcttccagc cagtggtgct tgagcaggct
     2161 cttgaactga ggatcaagtt cctggctgtt ccgcacgcct tcgatatagt gcctgagggt
     2221 gatccattcg atgtgcagga ttgcaatgcc ggtcccgaga gggctatgcg acagcacaaa
     2281 acgcgagata tcctccgcag gtccgatgaa gtcgcacttg ctgccgaagc cttcctcgaa
     2341 ctcttggcga aagcgcttga agaggtgaat gtgcttggct tcctcgccgg cgaattgaag
     2401 cagggcgcgc acgcggtagt cgtcgccgga aagttgagga cgggcatgat cgaccacata
     2461 cggcagaatg aattcctcga ccagaccgaa catcaacaga tactcatgac cgcggatctg
     2521 attgagcgtc acttgttcct ctggcgccag aaaggatagc tgttttacct gcgccaagga
     2581 ttccggcatg aacggtttgg tgaagtcgag tttttttgcg ccgccgataa ggtcttccac
     2641 cctccagccg atcctttcgg atgcggccag cgtcgattga taagagtaat ggttttgcat
     2701 gtctcacctc ccgtaagagt ttgtttgcaa acccttaacg cacctagtgt gccactaact
     2761 caactaacga cttgttgaac aattgccgag acttggagag ttgagtagga tcggggttaa
     2821 ttgtgagatc taacgagtcg tgagatttca catcgcgagt tgtgagatct cacgtattta
     2881 acttgcggtt ctggtgccat tggattgcga gcagaaaaaa ttcttcttgc accaaagcaa
     2941 gaataatgat cgtggcaatc tgatctacag ccgtcggcaa tgtcccgact catctcggcc
     3001 gctggatgcc gagagcgcgg atgcgggaat tgagagtaga aggattgatg ccaagcatgc
     3061 cggccgcgcc gtcttttccc gcgacacgcc agccggatga ttcgagagcg cgcagaatgt
     3121 tctcccgttc cagctgctga agctcgtgga tcggcagaat tcgctgcgcc gggtgtatag
     3181 cgctgtccag ggaaggtacc gtttcgataa cgacctctaa aggaatctcc ggcagagctc
     3241 ggtcaaggtt gatagagcca tcccgggagg taatcacagc gcgctcgatc acgttctgca
     3301 gctcacgcac atttcccggc caggaataag ccttcaaccg ttttttgatc ggttctgaaa
     3361 gaggctcgat acgcctaccc atacgctgcg cgaatttggc ggcaaactcc gatgcgagaa
     3421 tcaccacatc ttcgtttcga tcacgcaaag gcggcacacc gatcggaaac acgttcaaac
     3481 gatagtaaag gtcctcgcga aaatgccctt cgcgtatcgc ttttttgaga tcgcggttgg
     3541 ttgccgcgat gacacggaca ttaaccttgc gcagaagcga gccgccgacc ggagtgaatt
     3601 cgccttcctg taaaacccgc aacagcttgg cctgtagatc gagcgaaagc tcgcccacct
     3661 cgtcgaggaa aatcgtgccg ctgtcggcca aagcaaaaca gccctcgcgc ctctgcgtgg
     3721 cgccggtaaa ggcgcccttt tcgtggccga aaaactcgct ctccatgagt gcggctggaa
     3781 ttgcggcgca attaatcgtg atcaggggct tgtcggcgcg acggctggcg ccgtgaatcg
     3841 ccctggcgat caactccttg ccggtgccgg tttcaccaag aatcaggacc gtggtatcgc
     3901 cgaccgcgac ttgctcgata tcctccagca cggttttaag cgcagcgctt tgcccgataa
     3961 tttgctcaaa gccgccaagc ttgttgagcg cttcacggag atattcggct tccgcggtga
     4021 gagaaccgat tttacggtcg gcttcgagcc gctcactgat gtttcttaaa atcagcgtgt
     4081 aaaaggtttg ccggccgatc tcgaagcacg acaaagtcgc ttccgcggca aattcactac
     4141 cgccgctcac cagcgcgcgt aacccccccg gaacccatgc ggagcggcgt tccggcggac
     4201 agggcttgaa ttcatcgatg agccgcaaga ttttttgata gctcttgctg gaaacaaatc
     4261 gactgaattc atcgccaatt gcctggccgg ccggacattg aaacgcctgc tcggcagcgg
     4321 ggttcacccg ggtcactttg agattctggt caagctcgat aatcgcatcc atggcgctgt
     4381 caaacagacg gcgtagcttc tcctcacgct ctttaacctg tctttcaacg ctttgccgtt
     4441 gcaactcggc cgctgctctg gcggcaaaaa ttctaaataa agcgtgaacc ttgggttctt
     4501 tcggaattgg ccgtcgatcg atcaccgcaa gatgaccgag gattttgcca tccacgtctt
     4561 tcaaaggcat tccgagatag ctcaccgcgt taattgcggt tatgtccgga tcgtcgggaa
     4621 agagtttaaa aagattatcg ggaaaatgaa ctatatcggc gctgtcgatc actctttcac
     4681 acggcgtgcc ggcgatatcg gtctcatagt cttttatcca ctgcccgtcc atccaaaagg
     4741 caagtgcgcg cagccggcgt cgttcctgga tgtattcagt gacccaggcg ccatgagtgt
     4801 ttaacgctcg cgccaggttt tccacgagcg cggcaaaaaa ccgttcgccc gtctcatttg
     4861 cggttccttc aagtatcgtt cggagcgcgg actcttcatc cagcgcagcg aggggggata
     4921 ctcccggatc atgatggatc atagaaaaca cctgagcgcg gcccttggaa atctgaacgg
     4981 cagcctgagc tgcctcgttc tcagaacgcc gtaatatccg gggcgacttg tctctgtcaa
     5041 gcgccagaac tatgctgtcg acagcgcaat cgactgcacg taggaaacaa tgcagtccaa
     5101 cctagattcc aaacttactt atgtaatcac cggctggtaa tcgtcacgat gcccgcgaac
     5161 atgaggaggg cgccgaaaac gatgcgtcgg ttaaccgttt ccactttgcg caggaaaaga
     5221 acactcaaga gcacggtaaa cagcggggtg gtattcaaga gcggaataat cacggacaac
     5281 tcgcctcgcc ctaaagcgat aaagttgagc acttgagccg tcgttgccag gagcgccgcg
     5341 gcgagaaaaa atgaaaaacc ctgccggtcc aggcgcagcg actgggctct cccggtgctc
     5401 agcacaaaga ccaataacag aatcagcgaa accgtggtca cgatggctac cgcgacaaac
     5461 ggatcgggta gaattttcag cccttgcttg cgtaagcttt gcgagatcgc ggaaaccaag
     5521 gccgcgctga tcgggaaagc ggtatcaacg aggcgccact ttgccgcgcc atcctgccgg
     5581 ccggatatca accagagact ggtcatgatc aggatcgtgc cgatataaac caggactccg
     5641 ggctcctcgt gcaaaaaact cacgccaata atcgtcgcaa acattggttc ggtgccgcgc
     5701 aggggaccgg cgcgagcaac accgattcgg gtaattcctt catagaacaa agctcgcgcc
     5761 agcaacggtt ggatgcaacc gctgatgaca aaatagatca tcgccggcga ggacaagaac
     5821 tctaacgatg tgctagctat caggtagctc cacatgcagg cggtggagac ggtgaagctc
     5881 atcgccatcg ctgcgaagat gttggatcgc cgtaagccga gacgaattag aacagaatcc
     5941 gaggcccaac ccatggccga gaaaatggcg gtgatttcag caagtagaga catgggcgcc
     6001 aggatgtgag agggtccaga taaacccacg tcctgatcat ttgcaaccgc agaattcgtc
     6061 atgcggcgtg ttcgatccat cctgtattgc atagcaggga cgactcgact tagaatcggc
     6121 ccgatagagg cgaacggagc caacaactaa caggaggggt tatggaaaac ggcaaacaaa
     6181 ccgaagcgaa gatggttaaa ctgcgaactc aactagtaaa ggaggggcat acgcgcaact
     6241 tgctggcgga gacggatttg atgactctgc gtatccattg ctacgcgccg gggatcggcg
     6301 agaacgcgct ccactcgcac accaaagagg atcatatttt cctggttctc gaaggaaacg
     6361 cagaattcaa tactggacgc gacggcaaaa ccattctcaa cgcgagcaag catcaagcga
     6421 tcgtgctccc ggcaggttgc tattatcagt ttcgcaattc cggcgatact ccgctggtga
     6481 tgactcgaat cggcgccggt cccgataaac aggaccagcg gctaaacccg gacggcacgc
     6541 caattcccgg aagaactcat gagcaaggcg cggcaaagcc ggtgctgatt gagggagcct
     6601 ttttcgaata atgtgatgga agttacagaa ggcaattaca cgcaggaacc tggacggaac
     6661 tgcggcattg cggcaatgcc gcagttcacc ttcaagctat tcgcatgcgc tcgatgctat
     6721 gctgaagctc tctcagttcg gttatcaacg atttctgctt cagccggtgt cgggcaagat
     6781 gcggcttcat aagtgaactg ggataaatct tcgcgccgca ctcaacgcac tttgccgcat
     6841 gcaatttctt attggcgatt gatatggcca caaggttggt cacaagtact gttcgcattg
     6901 cactctcctc gatcaaaaag ctgcaacgct attcagcaat gcttatgcca ataacatctt
     6961 gcgaacgcac acaagcggcg atgcgggttt ttgatcgctt caatgttaga tcgggttgca
     7021 tcactgctaa ttctgacagc aatcgtgtga cgtcgtgaca tcctgtcaaa agagtttgat
     7081 gccggtagcg ctcagctgat aaacgccgat gcaatcgccg gaaacgatga agcgctgttc
     7141 gggagagttg cagcggcgct caggaatgac tgactaactt cttcactgat tcgacaatcc
     7201 gctttttgcc agggagaaat tctttctcca acgccgggct ggcgggaacc ggcgcaaatg
     7261 gcgcggcgac acgaagaatc ggcgcatcga gatagtaaag cgcctgttgt tgtactgcgg
     7321 cggtaatctc cgcgccaatc ccgccctgtt ccacggcttc atgcgcaacc accatgcgcc
     7381 cggtcttcct gacggactcg acgatagttc cgatatccag aggtgagacg ctgcgggggt
     7441 cgatcacctc tgctgaaatg ctgctggccg ccaattcatc ggctgcggtt aaagcggctc
     7501 caaccatatt tgccagcgcg acgatggtga cttgttgtcc ggtatgcaga accgccgcct
     7561 tgccgatcgg caccagatga tcgccgtcgg gcacgttacc caggccccgg tacaaaccac
     7621 ggtgctcgat gaagaccacc gggttatcgt cgcgaatggc ggccttgaga aggcctttgg
     7681 cgtcggcggg atttgacggc atgacaacct tcaatcccgg aacgtgcgcc agccacgatt
     7741 ccaagctctg cgaatgatgc gcgcccatgg cgccactgat gccgcattgc acgcgcacgg
     7801 ttaacgggat cgcgagctgg ccgccggaca tgtaatggag cttggccgca tgattgacca
     7861 gttgatcgag cgcgagtgtg atgaaatcga tgaacatgat ctcgaccacc ggacgcaacc
     7921 ctgccgtagc ggcgccgatg gccaacccca tgaccgtacc ttcgctgatt ggcgtgtcga
     7981 ccacgcgttg agtgccaaag tcttgcgcca atcctttggt tacgccaaag ggcccgccga
     8041 gcgaaacgtc ttcgccgaaa atgtaaaccg ccgagtcgcg gctcatctct tcgtgaagtc
     8101 cggcgcgaat cgcttctaag aaggtttgtt ccggcatatt cgattcggtt ccgaacgttg
     8161 aagaagaagt tgttaacttt ccgcctgagt tacctggcta cattgttccc gatttcgagg
     8221 tcaactaagg aaggcttcca gttgttacaa tgtcgtaact tgggtcgcga cttcctccat
     8281 cgccggccaa gaggatgaga gggaaaattc cgcggcttct tcaacccggg cgcgcgcttc
     8341 agattcaaac gcttgaaatc cctcgtccgt tattgttttc ttgcttttaa gcaggcgcgc
     8401 gaatcgggca atcggatctt tctttttcca ctcggaaagc tgtgacaact ctctatactt
     8461 ggccggatcg ccttcatagt ggccacgcag gcggtgcgtt agacattcga taaagaccgg
     8521 gcctttgcgt gcgcgagcga tcgcaagcgc cttgctcatc gccaggcgca cggctaaaag
     8581 atcgttgccg tctaccgtgc tgctggcaat tccatatgtc ttggcatgct gcgccagtcg
     8641 ctcgatcttg gtatgcgccg atagcggtgt gaattccgcg tagccattgt tctcgcatac
     8701 gaagatcacc ggtaactgcc acaggctagc gatgttcaaa gactcatgaa atggacccgc
     8761 ctggccggcg ccatcgccaa aaaaaaccac ggcgacctgg ccctgttttt tttccttggc
     8821 ggcaaatgct gcgccgagcg ccaacggtat cgttccaccg accactcctg tggcagtgat
     8881 gaagccggca tccagcgcga cgatgtgcat cgaaccgccc ttaccgccgc agtagccgct
     8941 agcgcggccg gcaatttccg ccatcagccg gttaatatcg gctcctttgg caagcgcatg
     9001 accatgagag cgatgcccgc cgaagacaaa atcgtcctgg cgcagcaggc tacagacgcc
     9061 gaccgcgacc gcttcttggc caatccccag gtgaagcaag ccgacgatct gcctttcggc
     9121 atagagcgcg gagacttttt cttcaaaggc gcgaatcaag cacatccgcc gcaggagagc
     9181 gacgaagtcc ttcgagcttt cttgtttgct tgccatggat gttcaggagc tgagtgaatt
     9241 ttaaaagaag gttgaaagaa ataattacct caccacgaag tgtatcatca gcttacaatc
     9301 gccaacacag aattaaacca gagtacgttc tcgagcccta attctcagac gagtgtgtca
     9361 ttcccgcatg cttctagcgg gaatccagac ggaattcgga ctggatcccc gattaaaacg
     9421 ttcggggatg acgcttttgc gaactcgcat cttttactca gccgcacctt tctaaggagt
     9481 accacgaagg attcagatca attttttgac ttcgtcgtct catgaccttc gtgttatcga
     9541 agcttctatt tgccctggtg ccagctgcgt tgtgttcttc gcggacgaat ggatgcagga
     9601 gagaggattt ttcagccaag aagcttaagc caccgccgcg cggatcgcat cgatgattcg
     9661 atcttctcgc ggtccgatgt attcttccaa tggcgggctg aagggcaccg gcacatccag
     9721 ggtggatacg cgacg
//
LOCUS       DASUVS010000080        22340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1558314, whole genome shotgun sequence.
ACCESSION   DASUVS010000080 DASUVS010000000
VERSION     DASUVS010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 22340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22340
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558314"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VF182_04580"
     CDS             <1..819
                     /locus_tag="VF182_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436383.1"
                     /translation="EDLLFLGVWAATVFVFFSVSAGKRPPYILPLYPPLALLITVWLR
                     DQTIGSLWKPRYFKFAAVLASLTGAVLAGGLAIYLTGIDIISMLQSVGLARQENIDAE
                     VLNLLSTLRDLGWLVPVSFAAAMVLWFSVARSLYRYRIDSAVTQMVLVSVLAIAFARS
                     LILPNLAKTESYKEFVQSATEAVAGGQSLIVFPRGIDPSPIIFYSQRKIEILPDDIAV
                     LQKKITQSNEFIIVEEGVWNSHVAGGSQLPVLDRSRGAGPDGDARLVLVRGYGN"
     gene            991..2151
                     /locus_tag="VF182_04585"
     CDS             991..2151
                     /locus_tag="VF182_04585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6436384.1"
                     /translation="MKIALVHKRLDLNGGTERDLYLTAEGLRDLGHEVHLFCSEFRVQ
                     IPAGVVAHRVPVMPFGRTARLWSFAVNGSKSAASADCDVVVGFGRILKQDVLRCGGGS
                     HRRFLERLGAEGGWRRRAWQKFSVYHQSVLAIERRQYHLDRFKKIIAVSGRVKDDLMR
                     TYSIPEHRIRVIYNGVDPVRFTPDRRQQARLAVRDQWGIPRAAAVVLFIGSGFRRKGL
                     DRLLSIWQSPRLQKTWLLVVGDDAGITGYQELAATVGGGRIIFTGRQTEVERYYAAAD
                     LIALPAVQEAFGNVVLEALSSGLPVLVSREVGAAEVLSGALVQGIVDQPENPVELERK
                     LVALLERAADPELIREAITAAARFSWQNHLRELEGCLSRVARSRFGDRANVA"
     gene            2141..2977
                     /locus_tag="VF182_04590"
     CDS             2141..2977
                     /locus_tag="VF182_04590"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HEX6436385.1"
                     /translation="MLPDFVLLRRRATSAWIDENFNTPPITELIWDPDRFFSDTRCVV
                     VKDQRKIKVGRVAVEVSGALHRIFIKRYNAFSWRARLLSIFTQSGGFRSLQGTAILRN
                     AGFATAKPIAAVESRRCGVVSKSFYLSEEIADGKTADSYWREDLTIAQPYRFRHRRRF
                     ISGLALTFQALHRQGIYHNDLKDANIIVVPEENGELFYLLDLEGTRRYSDLILRRRIK
                     NIVQLNDTLGRLLRRSEKMYFLKVYLDSSFGSKPARRRWISAVLEQSRQRDRRSRRRK
                     AL"
     gene            3017..3931
                     /locus_tag="VF182_04595"
     CDS             3017..3931
                     /locus_tag="VF182_04595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6436386.1"
                     /translation="MLSPKITILIPTHNRADSLRRLLDSLEASVGLEQTPVETIVVNN
                     HSTDATPQLLTEVSAKHRRFPIIVLEELKHGKSRALNLGLGCASGDLILLLDDDVSVD
                     TCCVAEHVAAYEDGSFDAIQGKVLAGLDPEGKPADMARLREYNIPMVDHGDAPCEVRG
                     FTGTNISFKREVFEKAGLFDTRLGPGASGFSEDTEYSRRIRKNGFKIGYAPKAIIYHE
                     LNPERYGSSYNRNVQFRRGISRSIYRKDSLLFRVLPDLIGNCLRYLAYSATGRRQKAY
                     KTQGQIAKNYGYIAGKVQKRVLHHRRPR"
     gene            4099..4965
                     /locus_tag="VF182_04600"
     CDS             4099..4965
                     /locus_tag="VF182_04600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6436387.1"
                     /translation="MERADFAPRVNRVTLSSEAMNDGQSRSASKPSVSAIIVCYNEED
                     NIGRCLNSLRWCDEIVVVDSFSTDRTVEICRQFTNIVIQREWAGYRDQKVFAQSKTTK
                     DWVLMIDSDEEVTPDLQKEILEQLAAADSSCAGFLIARLVFYLDRWWRRGGWYPDYGI
                     RLFRRDCARWAGNEPHAKIIVNGPARCLRNPLHHFSYRDIDDHIQRINRFTSISSKEL
                     RSQGGRWRLTDALLRPAFKFLKSYLLKRGFMEGFAGFHVAATAAMYVYLKYAKLWELE
                     IKEKKEVGRKRL"
     gene            4946..6049
                     /locus_tag="VF182_04605"
     CDS             4946..6049
                     /locus_tag="VF182_04605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6436388.1"
                     /translation="MGGSGYSRLLRILHIDPEKNWGGGEAQVLGLLQNLSKWGHRNDL
                     ATPADGELFRQSLPSVRKIPLSIRNDLDVRGVPALRRLIRRERYDIVHLHTKRAHALC
                     VWLPRGERRPMYIVTRRMDYPEPNNCYTRYLYNRRVDGVVAISRSVLNCLLAAGVDRR
                     SIRLIYSGIDPLPFRALPSAEQRANDSAVVAMLAVLEERKGHRFLLEAARVLKDRGRK
                     IRYVFAGDGSLKTQLQDLAQALGLREEVSFAGFVKDVPKFLSSIDLLVLPSLFEGLGV
                     AALEGMASGKPVVATRVGGLAESIIDGETGFLVPPRNGVALADAIERIVADPSMARVM
                     GQKGAARVLSHFTIEQMARQNEAYYYALLDGTA"
     gene            6072..7064
                     /gene="rfaE1"
                     /locus_tag="VF182_04610"
     CDS             6072..7064
                     /gene="rfaE1"
                     /locus_tag="VF182_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEX6436389.1"
                     /translation="MFGSQRLAGILKNFRKIHLLVVGDLMLDRFIWGEVERLSPEAPV
                     PVLRVCSESCRLGGAANVVHNVRSLGAQVTACGVVGTDGAGETLLDELQRIGASTGGV
                     CADREVHSIQKTRIIARPSHQQIVRLDRENHTPIRDATLERICRFIVTKAARFDAILV
                     SDYGKGVVHPNLLKCLAGLAADKKLPLVIDPKKENFERYQRASLITPNKDEASAASGV
                     AIRDDQTLTMAGQRLLQMWRAKAVLITRGPEGMSLFRPRQAAQHFPTQPREVFDVTGA
                     GDTVVAVCALALAAGARYEEAAVLANHAAGLVGDEIGTVAVPLARLDRIIRDLP"
     gene            7061..7927
                     /locus_tag="VF182_04615"
     CDS             7061..7927
                     /locus_tag="VF182_04615"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX6436390.1"
                     /translation="MKSMTGYGEASLQNKYAKITVQVRSLNHRHLDLQLRVPREYLGF
                     EEEIRKSLRETVSRGRIDLFVNRTSAPGHARAVELDEELLGKYLKAIQRAKNKFHLEG
                     EIGVSIFSSVPELLRVRDIEINLRTEKQAVFAALRSALQKLEESRSREGRQLRMDMES
                     QLRRLKNTANELEKHASQSASSVTKSSEAGNADGAATKVGHEGVDAGSAAFKGDINEE
                     VVRLKTHIAALGTILREANPIGKKIDFILQEIQRELNTVSAKVPFLAVVQLVLQGKER
                     VEKIREQTQNIE"
     gene            7924..8568
                     /gene="gmk"
                     /locus_tag="VF182_04620"
     CDS             7924..8568
                     /gene="gmk"
                     /locus_tag="VF182_04620"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX6436391.1"
                     /translation="MKTNRVINKRKGIVFILSAPSGTGKTTVISGVLERFPDISLSVS
                     YTTRAPRAGEAPGRDYHFISHAKFLAMTTKGEFAESARVHDFFYGTPARTLNRAMASG
                     RDLFLDIDVQGARQIKQKYPEAVSIFLLPPSWRELKRRLGGRATDDRAIIRRRLANAR
                     REIQDVIQYDYYIVNSDIREAVATLEAIILAERQKISRIRAWGLAPLIRPVKIS"
     gene            8585..10786
                     /locus_tag="VF182_04625"
     CDS             8585..10786
                     /locus_tag="VF182_04625"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX6436392.1"
                     /translation="MKIGDLLDKVRSYHPAADVEVIRKAYEFSAKVHQGQKRASGEPY
                     LIHPLAVANIISELKLDEPSIVGGLLHDTVEDTLTTLDELKGIFGKEITALVDGVTKL
                     SRTNFSSREEKQAENFRKMLGAMGKDVRVILIKLADRVHNMRTLDHMPVDKQILTAQE
                     SLDIYAPMAHRLGIAWIKTELEDLSLKYLHPEIYHQLKRNVARKKTDREKYIEEVNSI
                     IARKLESEGIEADVTGRPKHFFSIYQKMESQNLLYDQIYDLVGFRVLVDTTREAYEAL
                     GIIHSHWRPVSGRFKDYIAVPKPNMYQSLHTSVIGPHGERMEIQIRTHEMHRVAEEGI
                     AAHWRYKEGEDFHVSDIQRYTWLRQLLEWQQNLQDPQEFLHSLKDDLFSEGIYVFTPK
                     GDLLNFPKGSTVIDFAYRIHSDIGLHCSGARVNGQLVSLKYILRSGDTVEIITTPQQV
                     PSRDWLKLVKTPRAKSKIRNWLKAQQRERSVALGREILESDLHRHSMDYGILRTEQKL
                     QVLSKELGLRDEEALLAALGYGRITARQVLSKLVPPEKLESGHKQPESSLERLFRLAS
                     GKIKHLGIRVKGIDDVLVRFALCCHPLPGEQIVGFITRGRGVTVHTIGCPTILESDPH
                     RKIEVTWEENVQAPRPVKIEVSCVDQPGLLAAISAAITSAKANIARAQIRTFTGQKAV
                     NTFEVMIKNSEHLKQVLQNILQVKGVYKAVRARGRTGGMPGRDQAAKAEVQ"
     gene            complement(10804..10995)
                     /gene="rpmB"
                     /locus_tag="VF182_04630"
     CDS             complement(10804..10995)
                     /gene="rpmB"
                     /locus_tag="VF182_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX6436393.1"
                     /translation="MARTCSLCGKGRSVGNSVSHANNKTKRTWRPNLQRVKGNFDGQV
                     KRALVCTDCIHSGRVQKVA"
     gene            complement(10988..11467)
                     /gene="rfaE2"
                     /locus_tag="VF182_04635"
     CDS             complement(10988..11467)
                     /gene="rfaE2"
                     /locus_tag="VF182_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEX6436394.1"
                     /translation="MSRKLKSLEQIKAIVLGARSNGKRIAFTNGCFDLLHRGHVHVLR
                     EARACADLLIVGINSDESVKQIKGPTRPVLPELDRCELLGAMEMVDFVILFNDPDPYN
                     LIAAIRPDVLVKGGDWNSGKIIGADLVEEAGGSVMVVPYIKGFSTTEIIERITNSNG"
     gene            11626..13065
                     /locus_tag="VF182_04640"
     CDS             11626..13065
                     /locus_tag="VF182_04640"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6436395.1"
                     /translation="MEYQDLRDWIQIADEMGELKTLKGCDWNLEIGAITELVAHKEDG
                     PAVLFEDIKGYPKGYRVLSNSLSSRKRLALTLGLPSGDTKMDFVKIWKDNYKSIKPVP
                     AKFVKKSALFDNVYKDGDIDMFKFPTPFWHDKDGGRYIGTGSVDITRDPDEGWVNYGT
                     YRVMIHDKNKLGFYISPGKHGRIQREKYAANGKPFKIAMSFGHDPLTFLAGSIEVPYG
                     VPEYEFIGGLRGEPFEMIEGEYSGLPIPANAEIVIEGDVHFDNPTVEGPFGEWTGYYA
                     SAERAEPWVEVKRLYHRNDPIILGSPPGRPPAELGWYRSYLRSALIWDEMEKAGVPDV
                     KGVWLTVSGGSRLMMCVSIKQRYPGHAKQAAVVASQCHAGAYLGRFVVVVDDDIDPSN
                     TDDMIWAMSTRCEPEQDIDILRRCWSGPLDPVIHPTKKGFNSRAIIDATRPYEWMKDF
                     PPVAESSKEVLDATAKKWGAELFSANGKK"
     gene            complement(13363..13695)
                     /locus_tag="VF182_04645"
     CDS             complement(13363..13695)
                     /locus_tag="VF182_04645"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HEX6436396.1"
                     /translation="MRNCRATAATLSLLAILSGSCAAVHNPALDRARNAYERVRRDPA
                     VAGRAAVALEKTRSTLEEAERVWSEEKDANEVEHLVYLTEKRAEIALATARRRIAADE
                     IQQLKPQR"
     gene            complement(13713..14189)
                     /locus_tag="VF182_04650"
     CDS             complement(13713..14189)
                     /locus_tag="VF182_04650"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HEX6436397.1"
                     /translation="MGTTSYSNSRIIPHAKAQHHFWIVICLIFSAAFPFFGCSVLPSD
                     WGRPATPTDGAFSKAELSLRTAAEARADELAPMDLQRAKEKLENARRAMAAGKNTEAR
                     RLAEAADVEAELAQAKAEAEIMRMAADYIRRRGDVLRQELEGDAKRGSATEMSRDR"
     gene            14531..15292
                     /locus_tag="VF182_04655"
     CDS             14531..15292
                     /locus_tag="VF182_04655"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX6436398.1"
                     /translation="MFPYRDENETQRTALVTLTIIALNVFAWLFVQGAGSTLALARSV
                     CELGLIPGELTGSIAPGTQFSMGEGIACATDPGRQLSHVLTHMFLHGSWMHLLGNMWF
                     LWLFGNNVEDSMGRLRFIAFYLLCGLTAAAAQIITSPDSAIPMVGASGAISGVMGGYL
                     LLYPTVRVYTLLVLGFFITSMALPAWVMLGYWFLIQFVSGWVSFGGEGGGVAFWAHVG
                     GFVVGVVLVRLFARSDYVAEHKAHHWRPRRVMNSW"
     gene            complement(15411..16316)
                     /locus_tag="VF182_04660"
     CDS             complement(15411..16316)
                     /locus_tag="VF182_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6436399.1"
                     /translation="MFTKLLVPLDGSNTAEKALPYARYLAQEKNLVIELLSVVDVGNM
                     ATHMSAERALHLDTMVDDAVRASEKYLQTIAGTLSPARIKTRVESGRPEEVIIEKAAA
                     EQNTLIAMATHGRSGMNRWLLGSVAEKVLRGTTAPVLLIRAREDARSQDGPTFKSVIV
                     PLDGSDLAERVLPKAVEFAKQRDLEIVLIRTYHIPTTAYAGVEGYALPIDDLLRDMKE
                     EACKYLENKVTDVKKLGLSKVSFTAKEGLPADEIISLAKNLPGGLIAMSTHGRSGVRR
                     WVLGSVTETVVRHSENPVLVIRAST"
     gene            complement(16879..17055)
                     /locus_tag="VF182_04665"
     CDS             complement(16879..17055)
                     /locus_tag="VF182_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436400.1"
                     /translation="MAAAPNKDQRATIQNKIACIRPQSTITVWIRKGTIIAVLVSDMQ
                     SKASVDETEASALH"
     gene            17216..17596
                     /locus_tag="VF182_04670"
     CDS             17216..17596
                     /locus_tag="VF182_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436401.1"
                     /translation="MTSLDDDLARELEKIGEAEVRTRLSRGEFGMVGSSKSTAVEAWL
                     NSRESERRTALESKALSNAEEAYSTARTAHAAASEALFNSREAMTLGRLSAATSHRAN
                     VIAIVAMICSAVAITIALIIAIYK"
     gene            17759..18763
                     /locus_tag="VF182_04675"
     CDS             17759..18763
                     /locus_tag="VF182_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6436402.1"
                     /translation="MTSPLLSFFTNRSRRIVETVTPARTLADVVLPPQTRRTLEHALA
                     QVRNHELIFGRWGLGERHTAGRGLAFNFAGAPGTGKTICAEAIAHELGMKLLVVDYAQ
                     AESMWFGETPKNIVAAFRAAAEQSAVLFFDEADAIATRRSAGAILPHDRESNLTINVL
                     LRELEAFNGIVIFATNLAANFDPAFERRIRTHVLFDMPGVEERSRIWELQVHPKKTPL
                     GPDVDFRRLAERYAMSGGDIKNSVIKAAAAAAAEPGSDLVKQIGQPHFERAAEDVLSA
                     KSVMHQSLFAPGGVSASDPIGLRQNFEARWRTAIIAALGLAATALIAALAAIGLTLLR
                     "
     gene            18913..19299
                     /locus_tag="VF182_04680"
     CDS             18913..19299
                     /locus_tag="VF182_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6436403.1"
                     /translation="MAKILIVDDNPDVREVLQCQLRMLGYLVIAAENGYVAIEKAVVE
                     QPDLILMDIMMPEMDGWQASRAVRANPQTKNIPILASTAMSRRADLNACMEAGCNAYI
                     VKPFTMMELKEKIGDLITRYCGENHS"
     gene            19544..20560
                     /locus_tag="VF182_04685"
     CDS             19544..20560
                     /locus_tag="VF182_04685"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436404.1"
                     /translation="MSLNGKLLRQQLIAWAIITFSMTSANAQERPTILAGHGTLSGSI
                     LPLWVGTDAKLFEKHGVQVRPIYLPRAAGRPALVSGDIQVYFSAGPPVVQMRLSGGDV
                     AVTSCVVHKLTSKVMVSPAIQKIADLRGKVVAVANPGSASDFAAKLFMARQRMKPGQD
                     VSIIYSGSTSAAFAALANGRVHAIFASPPNDIQATAAGFKPLLELGDLNIPYAGNCTT
                     VMRSYTAKQPARMRSFVAGITEAVAYIRRRPTEARSILQKYTRVSDSAVLQHTYDSEV
                     QYMEPVPAPTAEGVRSILEHLGVTGQQAEAFIAEFIDNRFIKQVIDDGLLKEIYPDGV
                     PVKG"
     gene            complement(20642..21340)
                     /locus_tag="VF182_04690"
     CDS             complement(20642..21340)
                     /locus_tag="VF182_04690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6436405.1"
                     /translation="MKLKDKVAMITGGARGIGKAIALAYAREGARLAICARTESEIND
                     TVQEIQKLKAEGKGWTCDVSVEDSVKDFVGDVIKEFGRIDVLVNNAGVMTRPVPITEL
                     EVKKWDYTIAVNLRGPFLVTKSVLPAMIKQKSGSIINVSSMIGRGAYANFIAYATSKW
                     GVEGFTQTLAAEARSSNIRVNSVEPGYVATKLTGYSGSQPESVTDVFVYLASDEANGI
                     TGKMLSASGLKSQL"
     gene            21603..>22340
                     /locus_tag="VF182_04695"
     CDS             21603..>22340
                     /locus_tag="VF182_04695"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436406.1"
                     /translation="MDVLSTTGYQRRPPHLVAEYKGFFADEGLEVRFHETTYAPDHNR
                     GMAEGRWDFTLSSADTMIARTTTDGVDYLLFMQAEEGLSAYLIGQPEIESIEQLRGKL
                     LAGDPGDSNLDLIRKKILRRHGMDDNQYEIEIIGSSPKRLEAFLKGRVAAAMLTPPAS
                     DNALAVGGVLLANAEDYVPGWPLTCGWTLRRWLLEHHDLTVRFVRAWAAATDWLLIPQ
                     NRAETLELIMAKEGLNRNSAEQAFRKVV"
BASE COUNT         5061 a         5794 c         6282 g         5203 t
ORIGIN      
        1 gaggatttgc tgttcctcgg cgtgtgggct gctacagtgt tcgtgttctt ttctgtttca
       61 gccgggaaaa gaccgcctta tattctgcct ctttacccgc cgctggcgtt gctcatcacg
      121 gtgtggcttc gagatcagac tatcggaagt ctctggaaac cccgctactt caaattcgcc
      181 gccgtattag catctctgac cggcgctgta ttggccggtg gtttggcgat ctacttgacg
      241 ggaatagata ttatctcgat gcttcaatct gtcgggctag cgaggcaaga gaatatcgac
      301 gccgaagtct taaatctgtt gagcacgctg cgtgatctcg gctggcttgt tcccgtgtcc
      361 tttgcggctg cgatggtact ttggttttca gtagcgcgca gtctttaccg ctaccgcatt
      421 gacagcgcag tgacccagat ggtgttggtg tctgttctag cgattgcttt cgcgcgcagc
      481 ttgattctgc cgaatttggc gaagacggaa agctataaag aatttgtgca atcggcaacc
      541 gaagcagttg ccggcggtca gtcgctgatt gtttttcccc gtggtatcga tccttcccca
      601 atcatcttct atagccaacg caagatagaa atccttccgg atgacatcgc agtgctgcag
      661 aaaaaaataa ctcagtccaa tgaatttatt atcgtcgagg agggtgtctg gaactctcat
      721 gtagccggcg gttcacaact accggttctt gaccgaagcc ggggggccgg tccggatggc
      781 gatgcccggc ttgttctggt tcggggttac ggaaactgat gtgagacgaa ccacgggcta
      841 tgcggcggtt caatcgccgg cgtggactca cggtgtttgc gaccggtgga gtcttcagag
      901 gtagagaaca ctagaacctc aactcggaaa actcgcagct gtttcaccat ctggtaacgg
      961 cgcgaaaata gcctgtaagg cgtgcccact ttgaagatcg ccctagtcca taaacgcctg
     1021 gacctaaatg gcggcaccga aagggacctt tatctaacgg ctgaaggact gcgagatctc
     1081 ggtcatgaag tgcatctatt ttgcagtgaa ttccgggtgc aaatccccgc cggggtggtt
     1141 gcgcatcgtg tgccggtgat gccctttgga agaaccgctc gactgtggag ttttgcagtc
     1201 aacggatcga agagcgccgc ctcggccgac tgcgatgtcg ttgttgggtt tggacgtatt
     1261 ctcaagcagg atgtcctgcg ctgcggcggc ggttcccata ggcgtttcct ggaacgactt
     1321 ggcgccgaag gcggttggcg tcgccgcgcc tggcaaaaat tcagtgttta tcaccaaagc
     1381 gtgctggcaa ttgagcgtcg gcaataccat ctcgatcgat tcaaaaaaat catcgcggtc
     1441 tccggtaggg tcaaagacga tttgatgcgc acctattcaa tcccggagca taggatcaga
     1501 gtcatttata acggggtcga ccccgtacgt ttcactcccg atcgccggca gcaggcgcgg
     1561 ttggcagtcc gcgaccaatg gggaatcccc cgcgccgcgg cggtggtctt atttatcggc
     1621 agtggcttcc ggcgcaaggg gttggatcgt ctgctatcaa tttggcaatc gccgcggcta
     1681 cagaagacct ggttgctggt ggttggcgat gatgcgggca taacgggtta ccaggagctg
     1741 gcagccacag tgggaggggg gcggatcata ttcacgggta ggcaaactga agttgaacgc
     1801 tattatgccg cggcagattt gatcgctctg cctgctgttc aggaagcgtt cggcaacgta
     1861 gtcctggagg cgctttcgtc cggattgccg gttcttgtga gtcgcgaagt cggagcagct
     1921 gaagttttat cgggcgctct ggttcaaggc attgttgacc agcctgagaa tccagtagaa
     1981 ttggaacgca aattggtcgc gctgctggag cgtgccgctg acccggagct gatcagagag
     2041 gcgataacgg cagcggcgcg attctcgtgg cagaatcatc tccgggagtt agaaggttgc
     2101 ttatctcgag ttgcccgttc caggtttggc gatcgcgcaa atgttgcctg atttcgtttt
     2161 gctgcggcgg cgcgccacgt cggcctggat cgatgagaat ttcaatacac caccgattac
     2221 agagctaatc tgggatcccg atcggttttt ttctgataca agatgcgtcg ttgttaaaga
     2281 ccaaaggaaa atcaaagttg gccgcgttgc ggtcgaagtc tctggagcgc tccaccggat
     2341 attcatcaaa cgttacaacg cgttttcctg gcgcgctcgg ttactatcga ttttcacgca
     2401 atccggaggc ttccgatccc tgcaaggcac ggcgatttta aggaacgccg gcttcgcgac
     2461 cgcgaagccg attgcggcgg tagaatcacg ccgctgcgga gtggtaagca aaagcttcta
     2521 tctcagcgag gaaatagccg atggaaaaac cgccgatagc tattggcgtg aagatctaac
     2581 gatagctcaa ccttacagat ttcggcaccg acgccgcttt ataagcggat tagctctgac
     2641 tttccaggcg ctgcatcggc aggggattta tcacaatgat ctaaaggacg ccaatatcat
     2701 cgtggttcca gaggaaaatg gggagctgtt ttaccttctt gatctggaag ggacccgccg
     2761 atactccgat ctcattctca ggcgcaggat caaaaatatt gtccagctga acgacaccct
     2821 aggccgcctt cttcgtcgta gcgagaaaat gtatttcctc aaagtttatc tcgactctag
     2881 ttttggcagc aagcctgcca ggcggcgctg gattagcgcc gtactcgagc aaagcaggca
     2941 gcgtgatcgg aggtcacgac ggcgaaaagc gctttaagat atgcttgccg tgctcgcgtc
     3001 cacttgaact acgcagatgt tgtcccctaa aatcactatt ttaattccta cccataatcg
     3061 cgcagacagt ttgcggcggc tcttggatag tttggaagca tcggtaggtc tcgagcaaac
     3121 tccggtggaa actatcgtcg tcaacaatca ctcgaccgat gccaccccgc agcttctcac
     3181 ggaagtatca gctaagcata gaagatttcc gattatcgtg ttggaggagc ttaaacacgg
     3241 taagtcgcgg gcattgaacc tcggtctagg atgcgcctcc ggggacttaa tcttgttact
     3301 ggatgacgac gtgtcggtcg atacgtgttg cgttgccgag catgttgccg cttatgagga
     3361 tggatctttt gatgctattc aagggaaggt tttagctggc ctcgaccccg aaggaaaacc
     3421 cgccgatatg gcgcgactgc gagagtataa tattccaatg gtcgatcatg gtgacgcgcc
     3481 gtgtgaggtg cgcggattta ccggaaccaa tatctcattt aagcgcgagg tctttgagaa
     3541 ggcaggcttg ttcgatacca ggctggggcc cggcgcatcc gggtttagcg aggacactga
     3601 gtactctcgg cggatccgga agaatgggtt caaaataggt tatgccccca aggcgatcat
     3661 ctatcacgaa ctaaacccgg aacgatacgg cagttcctac aaccggaatg ttcaattccg
     3721 cagaggaatt agccgaagta tttatcgaaa ggattcgctg ctatttcggg tgctacctga
     3781 tctcatcggc aattgcttgc gctacctggc ttacagcgct actgggcggc gacaaaaggc
     3841 ctacaagacc caaggacaaa tcgcgaaaaa ctacggttat atagccggga aggtgcagaa
     3901 gcgggtgctt caccatagac ggccgcgatg ataactgcgc atcagagatc acgccttcga
     3961 tgcttgacct acttcagtcc agcctattcc tcgagttttg aagtttttcg gaaagttcag
     4021 gtcatccaaa tttagcagtc ctgtttttac ctggaggatc taggggtatc atgattggcc
     4081 gtgaggagtc ttcgagccat ggagcgcgcc gactttgcac cgcgagtcaa cagggtcact
     4141 ttatcttctg aagctatgaa cgacggacag agccgttccg cgtcgaaacc cagcgtttcg
     4201 gcaatcatcg tttgttataa cgaggaagat aatatcggcc gttgtttgaa cagcttgcga
     4261 tggtgcgatg aaattgtcgt agtcgattcg ttcagcaccg atcgtaccgt ggaaatttgc
     4321 cggcaattca cgaatatcgt gatccagcga gaatgggccg gatatcgcga tcagaaggtc
     4381 ttcgcccagt ccaagacgac caaagattgg gtgcttatga tagactccga cgaagaagtg
     4441 acgccggatc tgcaaaagga gatcctggag caattagcag ccgctgattc gtcttgtgca
     4501 gggttcttga tagcccgatt ggtgttttat ttagaccggt ggtggcggcg cggcgggtgg
     4561 tatccggatt atggtattcg gttgtttcgc cgggactgcg ccagatgggc tggaaatgag
     4621 ccgcacgcaa aaataatcgt gaacgggccg gcgcgctgcc tgcgaaaccc actacatcat
     4681 ttttcctatc gagatatcga cgaccatatc caacgaatta atcgttttac ctccatttca
     4741 tccaaggagt tgcgcagtca aggtgggcgc tggcgcctaa ccgatgcctt gctccggccg
     4801 gcgtttaaat tcctgaaatc ttatttgcta aagagggggt tcatggaagg ctttgccggc
     4861 tttcatgtgg ccgccactgc cgccatgtac gtctatttga agtatgctaa gctctgggaa
     4921 cttgagataa aggagaagaa ggaagttggg cggaagcggt tatagccgcc tgcttagaat
     4981 cctgcacatc gatcctgaga aaaattgggg aggtggcgag gcgcaggtac tcggtcttct
     5041 gcaaaatctt tccaagtggg gccaccgcaa tgatcttgcg acacctgctg atggagagtt
     5101 gtttcgacag tctctcccga gcgttcgaaa aattcctctg agcatccgta acgatctgga
     5161 tgtaagagga gttcccgcgc tccggcgtct aatccggcgg gaaagatacg acatcgttca
     5221 tttacatacc aaaagagcgc acgcgctctg tgtgtggctg cctcgaggcg agcgccggcc
     5281 gatgtacatt gtgacccgac gcatggacta tcccgagccg aataattgtt acactcggta
     5341 tctttataat cgccgggtag acggtgtcgt cgccatctct cgttcggttc taaattgttt
     5401 gctggcggcc ggtgtcgatc gtcgctcgat ccgattgatc tatagcggca tcgacccact
     5461 gccgttcaga gcgctgccaa gcgccgagca acgcgccaac gattcggcag tcgttgcgat
     5521 gcttgcggtt ctggaggagc gaaaaggaca ccgttttttg ctggaggcgg cccgcgtgct
     5581 taaagatcgt ggtcggaaaa ttcggtatgt attcgccgga gacggttctc tcaaaaccca
     5641 gctacaagat ctggcgcagg ctttagggtt gcgagaggaa gtatctttcg ctggcttcgt
     5701 taaagatgtc ccgaaatttt tgtcttcaat cgaccttctg gtgctgcctt cattgtttga
     5761 aggtctaggc gtggcggcgc tcgaaggaat ggcctccgga aagccggttg tagcaactcg
     5821 ggtcggcggc ctggccgagt cgattattga tggcgagacg ggattcttag taccgccccg
     5881 gaatggcgtc gcgcttgccg atgctatcga gaggatcgtt gcggatccct ccatggcgcg
     5941 ggttatggga caaaaggggg cagcgcgggt tctgagtcat tttacaatcg agcagatggc
     6001 acgtcagaac gaagcctatt attacgcatt gctcgacggc acggcataag gtgtctcaga
     6061 gagaataccc aatgtttggc agccagcgat tagcggggat cttaaaaaat tttcgcaaga
     6121 ttcatctatt ggtggtcggc gatctcatgc tcgatcgctt tatttgggga gaggtcgaga
     6181 ggctctcccc ggaagcgccg gtgccggtct tgcgggtatg ttcagagagt tgccgtctcg
     6241 gcggcgccgc caacgtggtc cacaacgtca gaagcttggg ggcacaggtg accgcgtgcg
     6301 gagtagtggg cacggacgga gcgggcgaga ccttactcga tgaactccag cgcatcggcg
     6361 catcaacggg cggggtatgc gccgatcgag aagttcacag cattcaaaaa acgcgaatta
     6421 tcgcccgtcc cagccatcag caaattgtcc ggttggaccg agaaaatcat accccaatca
     6481 gagatgcgac actggagaga atctgccggt tcatcgtaac caaagcggcg cgattcgacg
     6541 cgatcctagt gtcggattac ggtaaaggag tcgttcaccc gaacttgttg aagtgtctcg
     6601 ccggtctcgc cgcagataaa aagcttcctc tcgttatcga tcctaaaaag gaaaattttg
     6661 agcgctatca acgcgcgtca ttgatcacgc ccaacaagga tgaagccagc gctgcgtccg
     6721 gggtagccat tcgtgacgat cagacgttga cgatggccgg acagcgcttg cttcagatgt
     6781 ggcgcgcaaa agcagttctc atcacccgtg gcccggaagg aatgagtctc tttcgtccgc
     6841 gccaagccgc acagcatttt cccacccaac cacgggaggt tttcgacgtc accggcgcgg
     6901 gcgacacagt agtggccgtt tgcgctttgg cactggctgc cggcgcgcgc tacgaggagg
     6961 cggcggtatt ggcgaatcat gccgccggcc tagttggtga tgaaatcggc acggttgctg
     7021 tgccattggc cagacttgac cggatcatca gggacttgcc atgaagagca tgacgggcta
     7081 cggcgaagcg tcgcttcaga acaagtatgc caagatcacc gttcaggtga gaagtttgaa
     7141 ccatagacat ctcgatcttc agctaagagt acctcgcgag tatctgggct tcgaagagga
     7201 gatccgcaag agcttgcgcg aaaccgtcag ccgcggacgg atcgatcttt tcgttaacag
     7261 aacgtccgcg ccgggtcatg cgcgagcggt agagctggac gaagagctgc ttggcaagta
     7321 tctcaaagca atccagcgcg ccaagaacaa gtttcatctg gaaggtgaaa tcggagtttc
     7381 gatcttttcc agtgttcccg agctactgcg cgtgcgggat attgaaataa atctgcgcac
     7441 cgaaaaacaa gcggtgttcg ccgcgcttcg atcggcgctc caaaagcttg aagaatcgcg
     7501 cagccgtgaa ggacgtcagt tgagaatgga tatggagtct cagctgcggc gcttgaagaa
     7561 cactgccaat gagttggaaa aacatgccag ccagtctgcc tcttcggtga caaaaagctc
     7621 ggaagcaggg aacgctgacg gagcagcgac aaaggttggc catgaaggcg tggatgccgg
     7681 cagtgccgcc tttaaaggcg acatcaacga ggaggtggtg cggctcaaaa cgcatatagc
     7741 cgcattaggc acgattctcc gagaagccaa ccctataggc aaaaagatcg attttattct
     7801 tcaggagatt cagcgggagc tcaataccgt cagcgccaaa gttcctttcc ttgccgtggt
     7861 gcaactggtg ctacagggga aagaaagagt cgaaaaaatt cgcgaacaga cacagaatat
     7921 tgaatgaaga caaatcgggt catcaacaaa cgcaaaggca tcgtcttcat tctgtcggcg
     7981 ccttcgggaa cgggtaaaac caccgtgatc agcggagtct tggagcggtt tcccgatatt
     8041 tccctctcgg tctcctacac tacgcgagcg ccgcgcgcgg gagaagcgcc cgggcgcgat
     8101 tatcatttca tcagccacgc caagtttttg gcgatgacaa ccaaaggcga gtttgccgag
     8161 tcggcccgcg tccatgattt cttttatggc accccggcgc ggaccttgaa tcgagcaatg
     8221 gccagtggca gggatctctt cctggatatc gacgtacagg gcgcccggca gatcaaacaa
     8281 aaataccctg aagctgtatc gatcttcttg ctgccgccat cgtggcgcga attgaaacgg
     8341 cgtttgggcg gtcgtgccac cgatgatcga gcgatcatcc gccgccgcct ggccaatgcc
     8401 cggcgtgaaa tccaggatgt aattcagtat gattactata tagttaatag cgatatccga
     8461 gaggctgtgg cgactttgga ggcgattatt ttggccgaga ggcaaaagat ttcacggatt
     8521 agagcttggg gactcgctcc tctaataagg ccagtaaaga tttcgtaaga ttatgagtaa
     8581 ggacatgaag attggcgacc tgctcgataa ggtccggtca tatcatcccg ccgccgacgt
     8641 cgaggttatt cgcaaggcgt acgagttttc cgccaaagtg catcaaggcc agaaaagggc
     8701 gtcgggtgag ccttatctaa ttcatcccct ggcggtagca aacatcataa gcgagttgaa
     8761 gctcgatgag cccagcatcg tcggcggttt gctgcatgac acggtcgagg acaccttgac
     8821 aaccctcgat gagcttaagg gcatcttcgg caaggagatt acggctctag ttgacggggt
     8881 gacgaagctg agccgcacca actttagcag ccgcgaagag aagcaagcag aaaactttcg
     8941 caagatgctc ggcgcgatgg ggaaagatgt tcgggtcatt ctcatcaagc tagccgaccg
     9001 tgttcacaac atgcgaacgc tcgatcacat gccggtcgac aagcagattc tgaccgctca
     9061 agaaagtttg gatatctatg cgccgatggc gcatcggctg ggaatcgcct ggatcaaaac
     9121 cgaacttgag gatttatcgc tcaagtactt gcatcccgaa atctatcatc agctcaagcg
     9181 caatgtcgcg agaaagaaaa ccgatcgcga aaaatatatc gaagaagtga attccattat
     9241 tgcgcgcaaa ctcgaatccg aggggatcga ggccgatgtg accggcaggc ccaagcactt
     9301 tttcagtatt taccagaaga tggaaagtca gaatctgctc tacgatcaaa tctacgatct
     9361 cgtcggtttt agggttctgg tggataccac gagggaagct tacgaagcct tgggtatcat
     9421 tcattcgcat tggcgtccgg tttcggggag gttcaaagac tatattgcgg tgcctaaacc
     9481 caatatgtac caatcgctgc acaccagtgt tatcggtccc cacggtgaaa gaatggaaat
     9541 tcaaatccgg acccacgaaa tgcatcgagt ggcggaagaa ggcatcgccg cgcattggcg
     9601 ctataaagaa ggagaagact tccacgtcag tgacatacaa cgatacacct ggctgcgtca
     9661 gctgcttgaa tggcaacaga atctgcaaga cccgcaagag ttcctgcaca gcttgaagga
     9721 tgatcttttt tccgaaggga tttatgtttt tacgccgaag ggcgatttgc tcaacttccc
     9781 taaaggctcg acggtgatcg acttcgctta ccgcattcac tccgacatag ggctgcattg
     9841 ctccggagcg agggtcaacg gccagttggt gtcgttaaag tacattctac gaagcggcga
     9901 cactgtcgaa attattacca caccgcagca ggtcccgagt cgcgattggt taaagctggt
     9961 caaaacaccc agggcaaaat cgaagataag aaattggctt aaggcgcagc agcgcgagcg
    10021 cagtgtcgcc cttggacggg aaattttgga gagcgatctt catcggcata gcatggatta
    10081 tggaattttg agaacggagc aaaagctcca ggttctgtcc aaagaactcg gcctcaggga
    10141 tgaagaagca ttgctggcgg cgctcggcta cggtcggatc accgctcgcc aagttttatc
    10201 aaagctcgtg ccaccggaaa aactcgaaag cgggcacaaa cagcctgaaa gttcgctcga
    10261 acggctcttc cggctggcat caggaaaaat caagcatctc gggattcgcg tcaaggggat
    10321 cgatgacgtg ctggttcggt ttgcgttgtg ctgccatccg ctacccggcg aacagattgt
    10381 cggcttcata acccgcggca ggggggtgac ggttcatacg atcgggtgcc caaccattct
    10441 tgaaagcgat cctcatcgga aaatcgaggt tacgtgggag gaaaacgttc aggcgccgcg
    10501 tccggtaaag atcgaagtga gttgtgtgga tcagcccggt ttgctagccg cgatcagcgc
    10561 cgcgatcacg agcgctaagg ccaatattgc gcgggctcaa attcgaactt tcacaggcca
    10621 gaaggcagtt aacactttcg aggtcatgat caagaactcc gaacatctta aacaggtgct
    10681 gcagaatata ttgcaagtta aaggcgttta taaagcggtt cgcgcgcgcg gccgcaccgg
    10741 tggtatgccg ggaagagatc aggcagccaa agcagaagtt cagtagggtt ctggattcga
    10801 actttatgcg actttttgaa cccggcctga atggatgcaa tcagtgcaca ccaaggcgcg
    10861 tttaacctga ccgtcgaagt tgcctttcac gcgctgcaag tttggacgcc atgtgcgctt
    10921 ggttttattg ttcgcgtgac tgacactgtt tccgaccgag cggcctttgc cgcataggga
    10981 gcaagttcta gccatttgag ttcgttatcc tctcgattat ttctgttgtt gaaaaacctt
    11041 ttatataggg aacgaccatt acgctgccgc cagcttcctc taccaagtcc gcaccgatga
    11101 tcttcccgct gttccagtct ccacctttga caagcacgtc cgggcgtatc gccgcgatca
    11161 gattataggg gtccgggtca ttaaacagaa tgacgaagtc gaccatctcc atcgcgccca
    11221 acaactcgca ccgatccaac tccggcagca ccgggcgcgt cggacccttg atctgcttga
    11281 ccgattcatc gctgttgatg ccgacaatca gcaaatctgc gcaagctctt gcttccctta
    11341 gaacatgcac atggccccgg tggagcaagt caaagcaccc gttggtgaaa gctattcttt
    11401 tgccattgct gcgtgctccg aggacgattg ccttgatctg ctcaaggctt ttgagctttc
    11461 gcgacacaca caacgggtag catgatcggc gattcttttc aattataact gcctgtcatt
    11521 tcgacgttat ccacttcttg accgccccga aaaatttcgt taaggtccga atactaaccg
    11581 agcgtcttcg aagcttcatc taccgagaaa atttgggagg tatcaatgga atatcaggat
    11641 cttagagatt ggatccaaat tgccgacgaa atgggggagc taaagaccct caaaggttgc
    11701 gactggaatc ttgagatcgg cgctatcacg gagctcgtcg ctcacaaaga agacggcccg
    11761 gcggtgcttt tcgaagatat caaaggatat cccaagggtt atcgcgtgct gtccaactcg
    11821 ctttcgtcac gcaagcggct ggcgctcaca ttaggtcttc cgtcgggtga caccaaaatg
    11881 gatttcgtca agatctggaa agacaattac aagagcatca agccggtgcc ggcaaagttc
    11941 gtcaaaaaaa gcgcgctgtt cgataacgtc tacaaagatg gcgacatcga tatgtttaaa
    12001 tttccgacgc cgttttggca cgacaaagac ggcggtcgtt atatcggcac gggcagcgtc
    12061 gatattaccc gggatcccga cgaggggtgg gtcaattacg gcacctaccg ggtcatgatc
    12121 cacgacaaga acaagttggg attctatatt tcgcccggta agcacggacg cattcaacgg
    12181 gaaaagtatg ccgcaaacgg caagccattc aagattgcca tgtcgttcgg gcacgatccg
    12241 ctgacttttc tagcgggcag catcgaagtg ccgtacggcg tgcctgaata cgagtttatc
    12301 ggcggcttgc gcggcgaacc gttcgaaatg atcgaaggcg agtattccgg cctaccgatt
    12361 ccggccaatg ccgagattgt aatcgagggt gacgttcatt tcgacaatcc tacggttgaa
    12421 gggccgtttg gcgagtggac cggctactac gccagcgcag aacgcgccga gccgtgggtc
    12481 gaggtaaaac ggctctatca ccgcaacgac ccgatcattc tcgggtcgcc tccgggacgg
    12541 ccgcctgcag agttgggttg gtaccgttcg tatctacgat cggctcttat ctgggatgaa
    12601 atggagaaag ccggcgtgcc ggatgtaaaa ggcgtgtggc ttacggtttc cggcggcagc
    12661 cgcctgatga tgtgtgtttc catcaaacag cgctatccgg gccatgccaa gcaggcggcg
    12721 gtggtggcgt cgcaatgtca tgcaggagcg tatcttgggc gctttgtcgt cgtcgtcgat
    12781 gacgatatcg atccatcgaa caccgatgac atgatctggg cgatgtcgac ccgctgtgag
    12841 cccgagcagg acatcgacat tctgcggcgc tgctggagcg gtcccctaga tccggtaatt
    12901 cacccgacca agaagggctt taattctcgg gctattatcg atgccacccg accgtacgag
    12961 tggatgaaag attttccgcc ggttgcggag tcgagtaagg aagtgctgga tgcgaccgct
    13021 aagaagtggg gcgcagagct gttcagcgcc aacggcaaga agtagcagtt aagtatttat
    13081 tcaacaaagg ggcaagtcga tttaatcgac ttgccccttt cagtttgtgg ttattgcttt
    13141 tagtgccttc ctgccgcaga caaactagtg ggcattcatt gcgcacgcac ccgtgttgca
    13201 gccgatgccg tgtagcggag ggaggccgag cttttcgaga agtacggttt agattccagc
    13261 tcgctcacat ttcgtcgccg gtgtggtgac cgcggtcaca ttcagcggca acagtatgtg
    13321 gggggtcagc ggcgtaaagc cattgtgcgc gcgccgcata ctttaacgtt gcggtttgag
    13381 ctgctggatt tcgtcggctg ctatcctgcg tctcgcggta gcgagtgcga tttccgctct
    13441 tttttcagtg agataaacca gatgctcgac ttcgttagcg tccttttcct cggaccaaac
    13501 gcgttcagct tcttcgagag tggaacgggt tttttctaaa gcaaccgcgg cgcgcccggc
    13561 aaccgccggg tctcgccgca ctctttcata ggcatttcgt gcccgatcca acgccgggtt
    13621 atgcaccgcc gcgcatgagc cggaaagaat ggcgagcaac gatagcgtcg ctgccgttgc
    13681 tctacaattg cgcaaagtat tctccggact catcatcgat ctcttgacat ttccgtggcg
    13741 gatccgcgct ttgcgtcgcc ttcgagttcc tggcgaagta catcgccgcg ccggcgaatg
    13801 taatccgcgg ccatgcgcat gatctcggct tcagcctttg cttgggcaag ctccgcttct
    13861 acgtcagcgg cttcggcaag acggcgagct tcggtattct tcccggcagc catcgctctt
    13921 ctggcgtttt ccagtttttc tttggctctt tgcaggtcca tcggagcgag ttcgtccgcc
    13981 cgggcctcgg cggccgtgcg caagctgagt tccgcttttg aaaaggcgcc atctgtcggc
    14041 gtagccgggc gcccccagtc cgagggcaag acgctacagc cgaaaaatgg gaatgccgct
    14101 gaaaagatca ggcatataac gatccagaag tgatgctgtg ctttggcgtg tgggataatg
    14161 cgcgagttgc tgtaagaggt cgtgcccatc ggtctccttt caaagttgtc tatttaacag
    14221 ccaagtatga cttgctaggc aaaaaacgcg caatccacct gatgccaaat tgtataacgc
    14281 tgaaaattaa ctacaagact tgggcggcgg taacgccaat cacttgcgag aaccaaaatc
    14341 gagagaaggg ccgggctggt cctatgcgca catcggctta gacaaggtat gacgtatctc
    14401 aatgtacgca acactagcgc aagtatagtc ctccagatat gttgaagtag aacgcttgca
    14461 atcatgcaat ccgagcggtg tacgtaaata gtactctcgt cgggagttag attaagagag
    14521 aggagcgcgc atgtttcctt atcgcgatga aaacgagacc caacgtacgg cgctcgtgac
    14581 gctgacaatc attgcgctaa atgtgtttgc ctggctgttc gttcaagggg ccggtagcac
    14641 gctggcgctg gcgcgctcgg tatgcgagct cgggctgata cccggagaat tgacggggtc
    14701 gatcgcgccc ggtacgcaat tttcgatggg ggagggaatt gcctgcgcta ctgatcccgg
    14761 ccgtcagctc tcccatgtcc tgacccacat gttcttgcat ggctcgtgga tgcatctgtt
    14821 ggggaacatg tggttcttat ggctgttcgg caataatgtc gaagattcca tgggacgatt
    14881 gcggtttatt gctttctatc tcttgtgcgg tttgacggct gcagccgccc agatcatcac
    14941 cagccccgac tcggcgattc cgatggttgg cgcctccgga gcgatcagcg gagtgatggg
    15001 cggctatctg ctgctttatc caacagtgcg tgtctataca ttgctggtcc ttggtttctt
    15061 tatcacctca atggcattgc cggcatgggt gatgctcggt tactggtttc tgattcagtt
    15121 cgttagcggc tgggtttctt ttggtggaga gggaggcggc gtcgctttct gggctcacgt
    15181 cggtggattc gtagtcggag ttgtgctcgt gagactgttt gcgcgctccg attatgtggc
    15241 tgagcataag gcccatcact ggcggccgcg ccgagttatg aatagttggt gaccggcgca
    15301 agccgtgcaa ctggagaatg agctactagc cgacagcgct atgcagattc gacgcacaac
    15361 gtctcattga tccattgagc cgcagcgcaa gtcgcggtca acgtcggctt ctacgttgaa
    15421 gctcgaatca ccagcactgg attttcggaa tgccgcacca ccgtttctgt gacgctgccc
    15481 aacacccagc gcctcacgcc ggaacgcccg tgagtggaca tagcaatcag gccgccgggg
    15541 aggtttttgg ctaaggatat gatttcatcg gcgggcaagc cttccttggc cgtgaaggat
    15601 accttggaga gtccgagctt cttcacatcg gtcaccttgt tctcgagata cttacaggct
    15661 tcctctttca tgtctctcag caaatcgtct atcggcaacg catacccttc gactccggca
    15721 tacgcggtgg tcggaatgtg ataggtgcgg attaacacaa tctccagatc tctttgcttg
    15781 gcaaactcaa ccgctttcgg caaaactctt tcggccaagt ccgatccgtc caacggtacg
    15841 atcacggact taaacgttgg cccatcttgg cttctggcat cttctcttgc ccgaatcaat
    15901 aagaccggag cggtcgttcc ccggagaact ttttcggcga cgctgcccag cagccagcgg
    15961 ttcatgccgg aacgaccatg ggttgccata gcaatcagcg tattttgctc cgccgcggct
    16021 ttttcaataa tcacttcttc aggtctgccg ctctccaccc gcgtcttgat cctagctggg
    16081 cttaaagtgc cggcaattgt ttgtaagtat ttctcgctgg cgcgaacggc gtcatccacc
    16141 atcgtatcca ggtgcagcgc ccgttcggca ctcatatgag tcgccatatt ccccacatcg
    16201 acaactgaca ggagttcgat cacaagattc ttttcttgcg ccaaatatcg ggcatatggc
    16261 agcgccttct cagccgtgtt tgatccatcc agcggtacta aaagtttggt aaacatggtc
    16321 ttctccttga tcgtggtacg tctctcgtac gatgggcgcg ctgtgtgcga gacttacgca
    16381 atatcgccgt ccgtcccttc attgtctccg ttcaactttc ttataacgct gggtacatct
    16441 ttagcaaata agccgacaaa tcgctggcgc agggatggtc gcaaattctt tggagaacat
    16501 ccagcgcttg cggatcatcc ttgttcatct ctggtgagga gcagcgcgat ctgccgcgag
    16561 tgcgcgggcg tcgaggtgga tttcagcagt ttgagccgtg ttaggcgaaa ccccgcgcgc
    16621 catttcttac aaggtacccg gcttcgtaag caaagtggtc gctcgcgggg agacggcccg
    16681 atggttctca gattcgaatg tctcatgcct ttgttctcgg ctcaaaacga ccgatcatcg
    16741 agattgcgcg tggttagcta gcgacgagaa ttgcccttcc gtctggagtc cgtccaggtc
    16801 atgatcagat cgcaaaccac gataaagatc aacagtgtca cgacatacag cagttctgtt
    16861 tcatgtgaca tagaattctc aatgaagcgc actggcttcg gtttcatcaa cgctggcctt
    16921 tgattgcata tcactgacta atacggcaat aattgtgcct tttctgatcc agaccgtgat
    16981 cgtcgactgc ggccgtatgc aggcgatttt attctggatt gtcgcgcgct gatccttgtt
    17041 cggcgcggct gccatacaag gttttttcat gcttgcgaat aaccggcaga ggtttcttgg
    17101 gagtgcgaag aaattactga ggaggggagc taaaacaggt cggcaaccga acggcaatgc
    17161 ctgatgtggt tggacggctt ttttgataag ctgagctatt taaacgagtg agccaatgac
    17221 ttccttggac gacgatctag cgcgcgagct ggagaaaata ggcgaagcgg aagtgcgcac
    17281 gcggctgtca cgtggggagt tcggcatggt tggtagctcc aaatcgacag ccgttgaagc
    17341 atggttgaat tccagggagt cggaacgccg gaccgctctg gagagcaaag cgctgtcgaa
    17401 tgccgaagag gcttattcca ccgcccgcac ggcccacgcg gcggcttccg aagcactatt
    17461 taattctcgt gaagcaatga cactcggccg gttgagcgca gctacttcac atcgggcaaa
    17521 tgttatcgct atcgttgcga tgatttgcag cgccgtcgct attacgattg ctctaatcat
    17581 cgcgatttat aaatgactgg cgccagattt attgtgtcta ggcgataaaa cgcggcactc
    17641 gagtcggtgg aacgacggca acgccttttg ttcatcgaac cggtacgtgg agtgtcgttt
    17701 atcagcgcga tgtgaaggag ttcgcgacaa gtctgtcgcc ctttgatgag aatcaatcat
    17761 gactagtcct ctgctttcat tctttacgaa tcgctcgcga cgaatagtgg aaaccgtcac
    17821 tccggcacgg acgctggcgg atgtcgtcct ccctcctcaa acccggcgga cattggagca
    17881 cgcgttggct caggtgcgga atcacgaatt gatcttcggc cgctggggtt taggtgagcg
    17941 gcacacggcc ggaaggggtt tagcctttaa ctttgccggg gcgccgggga cgggaaagac
    18001 catctgcgcc gaggccatcg cgcatgaact cggcatgaag cttcttgtag tcgactacgc
    18061 tcaagccgag tcgatgtggt tcggcgaaac gcctaaaaac atagtggcgg catttcgcgc
    18121 cgctgccgag caaagcgccg tactgttttt tgatgaagcc gacgccatcg caacgcgccg
    18181 ctcggcgggc gccattttgc cgcacgaccg cgagagcaat ttgaccatca acgttctcct
    18241 tcgcgagctg gaggcgttca acgggatcgt gatctttgcg acgaatctag cagcgaactt
    18301 tgatccggca tttgagcggc gcattcgaac tcatgtgctg tttgacatgc ccggagtaga
    18361 ggagcgttcc cgcatctggg agctgcaggt tcacccgaaa aagacccccc tcgggccgga
    18421 tgtggatttc cgcaggctcg ccgagcgcta tgcgatgagc ggcggcgaca tcaaaaattc
    18481 agtcatcaaa gcagcggccg cggcagccgc agaaccgggt tctgatctag tcaaacaaat
    18541 aggccagcca cattttgaac gtgccgccga agacgtgctt tcggcaaagt cagtgatgca
    18601 ccagtcgctt tttgccccag gcggtgtctc agcatcggac cctataggct tgcggcaaaa
    18661 ctttgaagcg cggtggcgga ccgccattat cgcggcttta ggactggccg cgacggcatt
    18721 gattgccgcg ctcgcggcaa tcggattgac cctcctgcgt tagtccaatc cgggcgcagc
    18781 cacgcacgca gcagcttctg agttatcggc cgatagtccg tcattaggcc ttcgcatcat
    18841 ttgcgcgttg caacaatctt aggcgataat agcaccccgg catgtcccgc gagcaccgcc
    18901 ggagtactta gcatggccaa aattttgatt gtcgatgaca accctgatgt acgtgaagta
    18961 cttcaatgtc agctcagaat gttaggctat ctcgtaatcg cagccgagaa tggctacgtc
    19021 gccatcgaaa aagcggtcgt agagcagccg gacctaattc taatggacat catgatgccg
    19081 gagatggacg gctggcaggc aagccgagct gtccgtgcca atccccaaac caaaaatatc
    19141 ccgatccttg catcgacggc gatgtcccgg cgtgccgatc tgaatgcctg tatggaagcg
    19201 ggttgcaacg cctacatcgt caaaccattt acgatgatgg aattgaagga gaagatcggc
    19261 gatctaatca cacggtactg cggtgagaat cattcttgat tacggtgcag cagtttctgc
    19321 tcagagaccc gagccaacac ccaacccgaa cagtcggtga tcttcgacct agcacacgct
    19381 ggccacctaa gttcaaaact gccactccag actctgacag cttgcaaact ggtgccaagc
    19441 caggcactca attatctggg tctattccac ctgcacgcta atccctaatc gcagctttga
    19501 tcaggcgcgc tttgcggtag cacgctccta cagtgaggtt gatatgagtc tgaacggtaa
    19561 gcttttacgg cagcaattga ttgcatgggc gatcatcact ttttccatga catcggccaa
    19621 cgctcaggaa cgaccgacta ttctcgccgg acatggaacc ctctcgggat cgatcctgcc
    19681 gctctgggtc ggcaccgacg caaagctgtt cgagaagcat ggcgtccagg tcaggccgat
    19741 ttatctgccg cgggccgcag gaagaccggc gctggtgagc ggcgacatac aggtgtattt
    19801 ttccgctggc ccgcctgtgg tgcagatgcg cttgagcggg ggcgacgtag ccgttacttc
    19861 ctgcgtggtt cacaagctca cgtccaaggt aatggtgagc ccggcaatcc aaaagatagc
    19921 cgacctgcgg ggtaaagtcg tcgcggttgc caacccaggt tccgcaagtg atttcgccgc
    19981 caaactattt atggcacgac agcgcatgaa gcctggtcag gatgtttcga tcatctatag
    20041 cggctcgacg agcgcagcgt ttgccgccct ggccaacggc cgagtgcatg ccatctttgc
    20101 gtcaccacct aacgatatcc aggctacggc agcaggtttc aagcccttgc tggaactagg
    20161 ggatctgaac attccctatg ccggaaattg cactacggtg atgcgttctt acacggccaa
    20221 gcagccggct cgcatgcgca gctttgtcgc cggcatcact gaggcggtag cctacatcag
    20281 aaggcgccca actgaagcca ggagcatctt acagaaatat acccgcgttt ctgatagtgc
    20341 ggtcctgcaa cacacctatg attctgaagt ccaatacatg gagcccgtgc ccgctccgac
    20401 tgccgaaggg gttagaagca ttctcgaaca tttaggagta actgggcagc aggccgaggc
    20461 gttcatcgct gaatttatcg acaaccggtt tatcaagcag gtcatcgatg atggtttact
    20521 caaagagatc tatccggacg gcgtgccagt taagggatga aagatgaaag tccaaagctg
    20581 aatgaacttc ggcgcgggat ctgtttactt cttcgcggcg ggtgaacacg cagtttcgcc
    20641 actacagctg tgacttcaat ccggaggcgc tgagcatctt accggtgatg ccgtttgctt
    20701 catcggatgc aaggtaaaca aaaacgtcgg tcaccgattc cggctgacta ccgctatagc
    20761 ccgttaattt tgttgccaca tagcctggtt ctacggagtt gacgcggatg tttgaagagc
    20821 gggcttcggc ggctagagtc tgggtgaatc cttccactcc ccatttcgaa gtggcatatg
    20881 cgataaaatt agcataggcg ccgcgtccga tcatcgatga aacgttgatg atggagccgc
    20941 ttttttgctt tatcatggcg ggcaagacgg atttagttac gagaaagggt ccgcgcaaat
    21001 taactgcaat cgtataatcc cacttcttca cttctagctc agtgatcgga accggccggg
    21061 tcatcactcc agcattattc accaggacgt cgatgcggcc gaactctttt ataacatcgc
    21121 cgacaaaatc tttaaccgaa tcttcgacgg agacgtcgca ggtccagccc ttaccctcag
    21181 cctttaactt ttgaatctct tgaaccgtgt cgttgatctc cgattccgtg cgtgcacaaa
    21241 tcgccagcct ggcgccttcg cgcgcgtaag ctagagcaat cgctttaccg attcctcttg
    21301 cgccgccggt gatcatcgcg actttgtctt ttagcttcat gggctgtcgt tggtagatcg
    21361 atggctttaa tgtctactgg atcgcttgcc aaagcaagat gatctgaatt ctcagtcgat
    21421 gaaacagcta tcaatgacag ttggatgaat tgggctttac ccgcgagcaa tatccggagg
    21481 cagacggcac ggcatacacc aatgtcgagg gcgaacagat gctgatcgaa ccgctccttg
    21541 ggacatcttg agcgtggccg cttctgggga tagaagagaa agaactcggg gaggaaacac
    21601 aaatggatgt gctcagtacc accggatatc agcgcaggcc gcctcacctg gtcgccgagt
    21661 ataagggatt tttcgctgat gagggactcg aggttcgctt tcatgaaacg acctatgctc
    21721 cggatcacaa ccggggcatg gccgagggac gttgggattt tacgctcagc agcgcggata
    21781 ccatgattgc gcgcacaacc accgatggag tcgattatct tcttttcatg caggcggaag
    21841 aggggctgag cgcgtacctg atcggccagc ccgagatcga gtcgatcgag cagctgcgcg
    21901 gcaaactgct tgccggtgat cccggcgatt cgaacctcga tctcattcgc aagaagattc
    21961 tacgtcgtca tgggatggac gataatcaat atgaaattga aatcatcggc agctcgccca
    22021 aacggctcga ggcttttctg aaaggtagag tcgctgccgc gatgttgacg ccgccggctt
    22081 cggacaacgc cttagcggtg ggcggagtgc ttctcgccaa tgctgaagac tatgttccag
    22141 gatggccgct gacatgcggc tggacgttgc gccgctggct gcttgagcat cacgatctaa
    22201 cagtgcgttt tgtacgcgca tgggcagcgg cgacagactg gttgctaata ccgcaaaatc
    22261 gcgcggaaac tcttgaactg atcatggcca aagaagggct caatcgcaat tccgcagagc
    22321 aggcctttcg caaggtcgtg
//
LOCUS       DASUVS010000081        16546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_28771, whole genome shotgun sequence.
ACCESSION   DASUVS010000081 DASUVS010000000
VERSION     DASUVS010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 16546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16546
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28771"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..1674
                     /gene="pfp"
                     /locus_tag="VF182_04700"
     CDS             346..1674
                     /gene="pfp"
                     /locus_tag="VF182_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HEX6436407.1"
                     /translation="MQKQSQPSTTLNAAVGVLVGGGPAPGINGVIGAVTLEARNRARK
                     VIGLYDGFQWLMRGDTTRTKELLKDDVSRIHFQGGSVLNTSRANPTKKMQDVERVVAT
                     LDRLGIGYLVTIGGDDTMYAASQVAKQAAGRIRVCHVPKTIDNDLPLPGEIPTFGFET
                     ARQLGSALVQNLMEDSRTTGRWYFVVVMGRTAGHLALGIGKSTGATLTIIPEEFPGTV
                     HLESVCDILEGAILKRTTLWNRSDGVAVIAEGLLERVPPEELQSIEGVRIAHDSYGHL
                     RLAELDLAYILKTLVEHRFANRGSSMSIVHKNIGYELRSAAPIAFDCDYVRTLGYGAV
                     EFLLNPDAKIANLTGALVCLVDGELKYLDFASLQDSKSGKTRVRSVGITKPTYKVARE
                     YMIRLEHEDFADSEKLRLLAQAASSKNKLCSPEEFKSRYQYLATDLLGSV"
     gene            1703..2716
                     /gene="gap"
                     /locus_tag="VF182_04705"
     CDS             1703..2716
                     /gene="gap"
                     /locus_tag="VF182_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX6436408.1"
                     /translation="MATRVAINGFGRIGRLVLRSIKQRYKKELAVVAVNDMADLHTNA
                     HLFRYDSTYGIYPGTVEAGEGMIRIDGDNIAVHNYKDPSRLPWKQLGVDIVIESTGMF
                     TDGAQVKAHLDAGAKKAIITAPATHEDITVVLGVNDSQYNPRQHHIVSNASCTTNCLA
                     PVAKVLHDTFGIERGLMTTVHAYTNDQRILDLMHKDLRRARAAATNIVPTSTGAAKAI
                     GLVMPELKGKLHGLSLRVPTPTVSVVDLVVNLKKPASADNINKALKNAASAKMAGYLE
                     YSDEPLVSSDFRGNPASSIVDGLSTVVLEDKMAKILSWYDNEWGYSCRVADLAILMAQ
                     KGL"
     gene            2726..3115
                     /locus_tag="VF182_04710"
     CDS             2726..3115
                     /locus_tag="VF182_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6436409.1"
                     /translation="MFAKDIMTRDVITVSPTATVKNLAMTLIKNQISGAPVAARNGKI
                     IGVVSEADIVAKKGKDVKTIMSKKIISVTEETAVEEIAKLMTTHGIKRLPVMRGESVV
                     GIVSRADIVNAIALGKHVALHTPVYDL"
     gene            3419..4531
                     /locus_tag="VF182_04715"
     CDS             3419..4531
                     /locus_tag="VF182_04715"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEX6436410.1"
                     /translation="MPQKISTFDDWTDYFSKWQKDIGFDASQIDDYKFDAFYADPPHT
                     EIEFGEYKGRKKWEKLLDVPTQEMRDALMHLIVYQGDTEFASTEQQRDLLQSAPSEYD
                     RQCLLRVMREEQRHGWQMCHVLVKNFGDSGKLEARKLLERRAYQGTRLLGAFNQPMEH
                     FLEFFVYTCFIDRDGKYQLTMLQHSGFAPLAQSMGPMLKEEAFHLFTGQTGLSRVLKA
                     GKVPIDIVQKYLNKWLSTGQDLLGKDRSSSANRFYRWGFKGRFDEDTAKEAPKDLDCI
                     NEESRAHYYQECSDIIDILNRQIVAGQPRLRAPDPKFNRQIGEFAGKTYSTDGDLLSA
                     GEYQNYINRVLPQPHDLERLRACTKEGGWVNGETFQ"
     gene            complement(4695..5705)
                     /locus_tag="VF182_04720"
     CDS             complement(4695..5705)
                     /locus_tag="VF182_04720"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436411.1"
                     /translation="MKKLFICFIALEVFYALSANTAFSAAPLTKVVISYAVLSEREGA
                     LFVARDQGFFRKQGLDVDLVYVSSAPVALASIAHGDSQINTASVSGAILGAMAGGLDL
                     VFIAGLVNRLTGTIVSSPEIKSPAELKGKTIGVTSIGGGNWVFTMLALEHWGLDVKRD
                     NITIRVIGNDAVRAQAMTTGVIHATQLSAYSSAAMLKRQGYRVLADLPELGIPYQGTT
                     VFARRSYVNQQPEVVEKVLTAIVEAIAFVQDPANKAAVMRSLAKGLRLPKPEDTADGY
                     EIIKTLYERKIYPTAEGIRNTIRLLGATNEKIRGLRAEDLIDDRTVRRLEEKGLFQAN
                     PR"
     gene            complement(5813..6808)
                     /locus_tag="VF182_04725"
     CDS             complement(5813..6808)
                     /locus_tag="VF182_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436412.1"
                     /translation="MPFRFYYSRLTAALLLSLLLALGSTAMAASVTKKISIIFADFSE
                     RSGLLFVAKDQGFFAEQGVDADIVQVRSGPVAISALAANEAQFYIVSATGSSLGAVAA
                     GLDLAYVAGLINKLDGYFVTSAKIRTPEDLKGKAIGVQSIGGGIWMFTQMALDHWALS
                     TERDKIQMRVLGDQSVLAQAMSTGLVDGAVLGYAFSQVVQRSGGRVLAELPRLNIPYQ
                     GVGLLARRSLINSSPDTVEKTLRALIKANHFIQDKNNQPAVLASLRKWLRISPNEGGQ
                     ELYDRMRILYDRRIIPTREGIQNALRVLSKADPKFAKLKADDLIDDRIARKLERE"
     gene            7224..8234
                     /gene="gap"
                     /locus_tag="VF182_04730"
     CDS             7224..8234
                     /gene="gap"
                     /locus_tag="VF182_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX6436413.1"
                     /translation="MTNVAINGFGRIGRLGLRAMLERHKEELNVVAVNDMADLHTNAH
                     LFRYDSTYGIYPGSVQVGEGQLKIEGWDIAVLCEKDPSRLPWRKLGVDIVIESTGLFT
                     SGVQARAHLEAGAKKVIITAPATDEDITLVLGVNESAYDPDKHHIVSNASCTTNCLAP
                     LAKVLHENFGVERGLMTTTHSYTNDQRILDLMHSDMRRARAGAANIVPTTTGAAKAIG
                     LVLPELKGKLHGLSLRVPTSSVSIVDLVVDLKKPASAAAINESLKSSADGDLDGILAY
                     CDEPLVSSDFRGNSASSIIDGLCTVQLEEKLAKILAWYDNEWGYSCRVGDLAAFMIER
                     EL"
     gene            complement(8295..9005)
                     /locus_tag="VF182_04735"
     CDS             complement(8295..9005)
                     /locus_tag="VF182_04735"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018924948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HEX6436414.1"
                     /translation="MLDGTPQIIPIGGGGFYRDPENLALERYILAQTGAAEPRVLFVP
                     TASGEPDHYVASFYAAFLKLQCRPSVLSFFKRTPDLRSLLLNQDVIFVGGGNTKSLLA
                     LWRDWGVPELLREAWQSGVILTGVSAGAICWFEQGLTDSFSDGLRPLGCLGFLPGSCC
                     PHYDGEPQRRPSYHRFLASGEISAGVAIEDWAAIHYKGTEIHRVIGAKPGVRAYSMRS
                     VYGSVQEVALPVKYLTQT"
     gene            complement(9058..9447)
                     /locus_tag="VF182_04740"
     CDS             complement(9058..9447)
                     /locus_tag="VF182_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEX6436415.1"
                     /translation="MRPTRSRSWFSRFAKSTAKITGKPVTFMIAVSIVVVWAIMGPMF
                     GFSDTWQLVINTGTTIITFLMVFLIQNTQNRDSEALQVKLDELIRATHGAQNALLDLE
                     ELEEEELDRIKAGYEKIAERARGELRS"
     gene            9637..10575
                     /locus_tag="VF182_04745"
     CDS             9637..10575
                     /locus_tag="VF182_04745"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6436416.1"
                     /translation="MATLITGLGLVGTSYAQLALKRGESIVFYDIAPRKDFLANKLGT
                     ANVTVVQRDVRDLPALIETILKNKIDTVIHTAGLIGGKVAQPVYTGMQINVMGTVNVA
                     EAVRLTGVKRLVQISTFGVYDRRGGEPGPIDESFHRGPGEAYGNSKVAKELMVEAYQR
                     LYGFELIILRLANVYGVGHFAGGSGGGEMVQNMLQTGIRGGVVKVPQEVARDFEYIYY
                     KDLGRALDKAAITPLSSPVTLNIGTGVVIKFDDLVATAEKLLPNLQVELIPGQKPRSA
                     KQPMVITRAKQFLGWEPEYDLERGFKDYIEELKALS"
     gene            complement(10612..13164)
                     /locus_tag="VF182_04750"
     CDS             complement(10612..13164)
                     /locus_tag="VF182_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEX6436417.1"
                     /translation="MALVSQQIAPTVSKAIHPSTWLFALTGSLLLGQSATALPFVLSG
                     DLLSLLLVPSLLLINPLAKLRWLAGVIVGRDLTSHCNASTVNYRRWALLALLAGSTFA
                     LGYFRHHGLLFPTFPANHLRSIMDENKDLYLEGVLLHEPEKLPTRSRWYLRAERIWQP
                     TGAEEISGDLLLTVRQLRREWHYGDRIRAQIRPTVPKDSGNPGGFNYATYLSRRQIYA
                     IAFLESDEKIELVERAPDRWRGAVENLRRKIRRYIETHFSPESAALMKALVVGDMGEI
                     SKKMRANFTAAGVNHVLSISGLHVAMLGLVVFWLVRWAGSFNVYLLLRCNLIKIATFC
                     SFIAVVFYTALAGAMVPTVRSAIMIGVYELAVLLDREEEVLTSLTFAALLIGLVWPGV
                     IADISFQLSFLAVFSIAWGMRQVFRTVAAPEKDRLPQERSRIKPKLRSIGMHLAVPLL
                     ATIGTGPLIAHYFGHLSLAGFVSNPIVVPLVGFVVVPLGLGISFISLAWPGLVTPLVS
                     VLEWVLWFSGLLVEWFARLPFAHISVPAPDLWEIGAVYLLIASVLWLRKSRRAVIAVA
                     LSASMLLADVVYWSRERWTRQELRVTYLNVGQGDAVVIEFPGAKTLLIDAGGTATGEF
                     DTGEAIVGPYLRSRKILKVDYLLVSHPRIDHYGGMGSIVREFTPDEFWSGAAKGRTRR
                     FEELDDILDRSKIQRVSLTNEAPCRSIERVKLCFLYPPADRNDDVSVVVQLHYGKARL
                     LFAGDIDKRDEQFLAQIPVRINSAVVKVPRHGSASASTPEFISAVQPRLAVVSGTTRN
                     VRPEKQELADRYRAAGADILRTEEDGAIIITTDGNDLRYESAKSGKRGELKL"
     gene            complement(13175..15019)
                     /gene="uvrC"
                     /locus_tag="VF182_04755"
     CDS             complement(13175..15019)
                     /gene="uvrC"
                     /locus_tag="VF182_04755"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX6436418.1"
                     /translation="MDEKLTNLPSRPGVYLMRDGAGKVIYVGKAKDLRARVRSYFNRN
                     DGRSQIEFLVRRVENLETLVTSNDKEALILENNLIKQYKPRYNIRLKDDKSYLSIKVT
                     TPDVWPRIFSTRKIVKDGSRYFGPFSSAVAARETVDIIEKHFLLRNCTDHNFKNRSRP
                     CLQYEIKRCLAPCVLPVDPEEYRQQVRQAILFIEGKQHELLAELRQRMAAKAGAMEFE
                     SAAKIRDQIQAVEKTLEKQRMVSHWGADQDIFGLYREGGYIEAQVLFVRQGKLTGTQA
                     YALEDLAFSDEEIMGSLLTQFYQGQRFLPDEILLPVELEDQDTRAEYLSERKGKNVSI
                     HVPQKGDKRKLVEMAQQNARQSFTERHDQEKARENMLQELQEHLRLKRYPQRIECFDI
                     SMIHGAYAVGSQVTFIGGEPDKAHYRHYRIRTIDPSGGGDDFGMMLEVLKRRFTRGQR
                     EEVMPNLVVVDGGKGQLAMALAAMREVGVEGIDVVGLAKMRVQSAPRSAEIERSEERV
                     FLPGQTNPVTLRRNSNALFLLQRVRDEAHRFAITHHKKLRSQQTLSSALDQIPGIGAA
                     RRRALLRAFGSVKGVEQATLDELMQVPSMNLKLAHQLWQVFHPAPPPQ"
     gene            complement(15180..16546)
                     /locus_tag="VF182_04760"
                     /pseudo
     CDS             complement(15180..16546)
                     /locus_tag="VF182_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469742.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
BASE COUNT         3904 a         4694 c         4360 g         3588 t
ORIGIN      
        1 gagcatccgt gtgttgcact ttatcctcgg aataaacttc gcactaaata tgctaccgcc
       61 ctacgacctt gttcgagact caacgttctt cgcgcggagc atcagtcagc tccagatcta
      121 gcatccccag aaaacgttcc acaacggctg tgcccatgca actccccgcg ctgatgcgct
      181 acaaggctcg gggcaacaaa aactttggcg aatttgcgga tggtaggtgt cagcttcttg
      241 cagccaagaa aaacttctct gcagatatct tggatttgcg tgccaaaacg ataaacttcg
      301 tgtagcattt tacccgatgt aaatcgcagt gcggcaggtt caatcatgca aaaacaatcg
      361 cagccttcga caactctcaa tgcggctgtc ggcgttctgg tgggtggcgg accagcgccc
      421 ggcatcaatg gcgtcatcgg agctgttacc ttggaagcgc gcaatcgcgc tcgcaaggtg
      481 atcggtctct acgatggatt tcagtggcta atgcgtggcg atacaacgcg aaccaaggaa
      541 ctgctgaaag atgacgtctc gcgcattcac ttccagggcg gctcggtcct caatacatcg
      601 cgcgccaatc cgaccaaaaa aatgcaagat gtcgagcggg tggtcgccac cttggaccgt
      661 ttaggcatcg gttacttagt gaccattggc ggagacgata ccatgtatgc cgcaagtcag
      721 gttgcaaaac aggccgccgg acggatccgg gtctgccatg ttccgaagac catcgacaac
      781 gatttaccac ttcctggcga gattccgacg tttggcttcg aaaccgcccg tcaactcggt
      841 agcgcgctgg tgcaaaactt aatggaagac tcgcgcacta ccggccgctg gtatttcgtc
      901 gtggttatgg gacgcacagc cggccatctg gctctgggaa tcggcaagtc caccggcgcc
      961 accctgacga ttattccgga ggaatttccc ggcaccgtgc atctggaaag cgtctgcgat
     1021 attctcgagg gcgcgatttt gaaacgcacc acgctgtgga atcgttccga tggtgtggcg
     1081 gttatcgccg aaggtttgtt ggagagagtc ccgcctgagg agctgcaaag catcgaaggc
     1141 gtgcgcatcg cccatgacag ttatgggcac ttgcgtttgg ccgagctaga cctcgcctat
     1201 atattgaaaa ccctggtcga gcatcgcttc gccaatcgcg gctcttcaat gagcatcgtg
     1261 cataaaaata tcggctacga gctccgttcc gcggcgccga ttgcgtttga ctgcgactat
     1321 gtgcgcaccc tgggctacgg cgcggtggag tttctgttga atccggacgc caagattgcg
     1381 aatctcaccg gcgctctcgt gtgtctggtg gacggcgaat tgaagtatct cgactttgcc
     1441 agcttgcaag attcgaaatc cggcaaaact cgcgtccgtt cggtcggcat aaccaagccc
     1501 acttataagg ttgcgcgcga gtacatgatc cgtctcgagc acgaggattt cgccgacagt
     1561 gaaaaactac ggctgctggc gcaggcggcc tcatcgaaga acaaactatg ctcgcctgag
     1621 gagttcaaaa gccgatatca atatctggca acagatcttc tcggcagcgt ttaatcgatc
     1681 gaataacctt taagggggat taatggcaac tagggtcgcg atcaacggct ttggccggat
     1741 cggcagattg gttttacggt caatcaagca acgttacaaa aaagaattgg cggttgtcgc
     1801 cgtaaacgac atggccgatc tccataccaa tgctcatctg tttcgctacg attcaaccta
     1861 tggaatttat cccggaaccg ttgaagccgg cgaagggatg attagaatcg acggggataa
     1921 tattgccgtt cataactaca aagatccgtc gcggcttccc tggaagcaat taggcgtgga
     1981 catcgttatc gaatcaacag ggatgttcac cgatggagcg caagtcaaag cgcatctcga
     2041 tgccggtgcc aaaaaagcga tcatcacggc accggcgaca cacgaggaca taaccgtggt
     2101 tctgggagtc aacgactcac aatataatcc acgccagcat catatcgttt ccaatgcctc
     2161 ctgcactacc aattgcctgg cgccggtagc taaggtcctg cacgacacat tcggcatcga
     2221 acgagggttg atgaccaccg tccatgctta caccaacgat caacgcattc tcgacctgat
     2281 gcataaggat ttgcgccgag cacgcgccgc tgcaactaac atcgtgccga ccagcaccgg
     2341 tgcggcaaaa gcgatcggcc tggtcatgcc ggaattgaaa ggcaagctgc acggcctgtc
     2401 cctacgggta ccaacgccga ccgtatcggt ggtcgacctg gtcgtaaatc taaagaaacc
     2461 ggcatctgcg gataacatca ataaagcctt gaagaacgcc gcctctgcaa agatggccgg
     2521 ctatctggag tattccgacg aacccttggt atccagtgat tttcgtggta atcccgcatc
     2581 ctcgattgtt gacggcttat cgacggtcgt gctcgaagac aaaatggcta agatactttc
     2641 atggtatgac aacgaatggg gctatagttg ccgggttgct gacttggcga tattaatggc
     2701 gcaaaagggt ttataggagg cgaccatgtt tgcaaaagac atcatgacta gagacgtgat
     2761 caccgtcagt ccgacggcta ccgtaaagaa tctggctatg acgcttatca agaatcaaat
     2821 cagcggcgcg ccggtagcag ccaggaacgg caagatcatc ggcgtggtct ctgaagccga
     2881 catcgttgcc aaaaaaggca aagacgtaaa aaccatcatg agcaaaaaga tcatcagcgt
     2941 caccgaagaa actgccgtcg aggagattgc caagttaatg accacacacg ggatcaaacg
     3001 tctgccggtg atgcgcggcg aaagcgtcgt cggcatcgtc agccgcgctg acatcgtcaa
     3061 cgccatcgcg ctgggtaagc acgtcgcgct ccatacgccg gtttacgatc tttgatcgac
     3121 acatctctgc atcacgcgtg tttcctgaaa gttcataact gcagcgccga gattcgatga
     3181 atccggtgct actctaggac ataggcacaa accatcgttt tccgctcctc acattcgcgt
     3241 acgtaagaaa gttttcgact gatttctcgc agactggaat aaagtcgacg ccagtgtccg
     3301 cccttcttca agaaacccgg cgtttccccg cacgatccgt ggcatctctt ttgcggaatc
     3361 cactctcatt ctgccgatat aattattcgc cgcgtaaaat atagaggagg aatgagagat
     3421 gccgcaaaaa atttctacct tcgacgattg gacagattac tttagcaagt ggcagaagga
     3481 tattggtttc gacgcatcac aaatcgacga ctacaagttc gacgccttct atgcggatcc
     3541 gcctcatacg gaaatcgagt tcggcgaata caaaggcaga aagaaatggg aaaaacttct
     3601 cgatgtgccc acccaggaaa tgcgggatgc gttgatgcat ctgatcgttt atcagggcga
     3661 caccgagttc gcctcgacag agcaacagcg agatctcctg caaagtgcgc caagcgagta
     3721 cgatcgccaa tgcttgttgc gcgtcatgcg cgaagagcag cgccatggct ggcagatgtg
     3781 ccatgtcctg gtgaagaatt tcggcgactc cggtaaactg gaggctcgga aactactcga
     3841 acgccgagct taccagggaa ctcgcctgct cggggctttc aatcaaccga tggagcattt
     3901 cctcgaattc ttcgtttaca cctgtttcat tgatcgcgac ggcaaatatc agctgacgat
     3961 gctgcaacat tccggcttcg cgccgctcgc gcaaagcatg ggaccgatgt tgaaagaaga
     4021 agcctttcat cttttcacgg gtcaaaccgg gctttccaga gttcttaaag ccggcaaagt
     4081 gcccattgac atcgtccaaa aatatctcaa caagtggcta tccaccggtc aggatttgtt
     4141 gggcaaggat cgatccagtt cagccaatcg gttttatcgc tggggcttca agggccgctt
     4201 tgatgaagac accgccaaag aagcgcccaa agatctcgac tgcatcaatg aggagtcgag
     4261 agcgcactac tatcaggaat gctcggatat tatcgacata ttgaatcgac agattgtcgc
     4321 cggccagccg aggctgcgcg cgcccgatcc aaagttcaac cggcagatcg gcgaattcgc
     4381 cgggaaaact tacagcactg acggcgatct actatcggct ggagaatacc aaaactacat
     4441 caatcgagtt ttgcctcagc cccacgactt ggaacgcctg cgagcctgca cgaaggaagg
     4501 cggctgggtg aacggcgaga ctttccagta gaagggcgat ttaactctgg cagatagcct
     4561 gatcgagtac tggtcgaatc cagtgctcca gagcgatgct ttgaccggca ctctggtaaa
     4621 taatgatcac ggcttaagcc gtgatcatgg aaacggcgac tatcgttctt gagctagggc
     4681 agacgagatt agcgtcagcg tgggttcgct tgaaacaatc ccttctcctc cagccggcgc
     4741 acggttcgat cgtcaataag atcttccgca cgcaagccac gaattttttc attggtcgcg
     4801 cctaagagtc tgatagtatt tcgaataccc tccgcggttg ggtagatctt ccgctcatac
     4861 agtgtcttga tgatctcata cccgtcggcg gtgtcctcgg gcttcggcag gcgcagaccc
     4921 ttggcgaggc tgcgcatcac tgctgcctta ttggccgggt cttgaacgaa agcgatggcc
     4981 tcgacgatgg cggttagaac tttttctacg acttcgggtt gttgattgac atagctgcgc
     5041 cgggcaaaca cagtcgtacc ctggtatgga attcccaact ccggcagatc agccaaaacc
     5101 cggtagccct ggcgtttcag catggctgcg gatgaatacg ccgatagctg ggtcgcatgg
     5161 atcactcctg tggtcatcgc ttgggcgcgg accgcgtcgt taccgatgac ccggatggtg
     5221 atgttgtcac gtttgacgtc gaggccccag tgctctagcg ccaacatcgt aaacacccaa
     5281 ttaccaccgc cgatgctcgt cactccgatt gtcttgcctt tcagctcggc cggggacttg
     5341 atctcgggcg acgaaacaat ggttccggtc aatcggttta ccaggccggc gatgaaaacc
     5401 agatccaagc cgccggccat tgcgccgaga atcgcgccgg aaaccgaagc ggtgttgatc
     5461 tgcgaatcac cgtgggcaat cgacgccaaa gccacaggag cgctggatac atagactaaa
     5521 tccacatcca acccctgctt acggaagaaa ccttgatccc gcgcgacaaa gagcgcaccc
     5581 tcacgctcgc tcaataccgc atagctgatc accacctttg tcagcggcgc ggcactgaaa
     5641 gccgtgttgg ccgacaaagc gtagaacacc tccaaggcga taaagcatat gaatagtttt
     5701 ttcataaaga ttcagctttc aaggcgaatt taaaggtaac gcaggcagcg acgtcaacat
     5761 cacgggacag cataaggagc aataagatca gcgacgagta tcgcacactc gattattccc
     5821 gctcgagctt gcgcgcgatt cggtcatcga tgagatcatc ggctttgagc ttggcaaact
     5881 tcgggtcggc cttactcagc acacgcaatg cattctgaat accctcgcgg gtcggtatga
     5941 tccggcggtc ataaagaatg cgcattctat cgtataactc ctggccgcct tcattgggcg
     6001 aaatccgcaa ccatttacgc aagctagcca gcaccgccgg ctggttgttc ttgtcctgta
     6061 taaagtggtt ggctttgatc agtgctctaa gagtcttctc cacggtgtcc ggggagctat
     6121 tgatcaggct gcgccgtgcc agcaagccga caccctgata cgggatattc agtctaggta
     6181 attcggccag cacccggccg ccgctgcgtt gcacgacttg gctgaacgca tagcccaata
     6241 ccgcgccgtc gactaatcca gtcgacatcg cctgagctaa caccgattgg tcgccaagaa
     6301 cgcgcatctg gattttgtct ctctcggtgc tcagggccca atgatccagc gccatttgtg
     6361 tgaacatcca gataccgccg ccaatgctct gcacaccgat ggctttgcct ttgaggtcct
     6421 cgggagtacg aattttggcg gacgtcacaa aatagccgtc gagcttgttg atcaacccgg
     6481 ctacgtacgc cagatccaat cccgccgcta cagccccgag cgacgaacca gtggccgaaa
     6541 caatataaaa ctgcgcctcg ttggctgcca aagctgaaat cgccaccggc ccgctgcgca
     6601 cttgcacgat atcagcgtcc actccctgct cggcaaaaaa accttgatcc ttggcgacaa
     6661 acaagagccc gctccgttca ctgaaatcgg cgaagataat actgatcttc ttggtgacgc
     6721 tggccgccat tgccgttgat cccaaggcta ataacagcga tagcaacaaa gcggctgtta
     6781 gccttgaata atagaaccga aagggcatta cgcacttcct ccttgaaagt ggaaacgggt
     6841 tcgtgatgaa accatttcaa taactcgatc gagtatcaga attcaatgct tcgacgaggc
     6901 agtcgttttg ttgaacgata cccaatttgc ctgggatatc tccactctgt agacctattt
     6961 gtctcctact gaaccacccc tgcgagatgc tgtgatcaac ttagatcctt tcgcgatgtg
     7021 tcaaacatgt acaattcttc tctattgtgc acaaaaatct cacatccatt gattagattt
     7081 tcgtcagcgg cgaatccgct ggagcgcttg ttcctgggta aaaagcgttg ctgacgcaga
     7141 tttgattcag cggaataaac tttgctttga tttttgcctt cataccctcg acaaattcgc
     7201 ccctaaaatc tgcaggagga caaatgacca atgtagcgat caatggattc ggtcggatcg
     7261 gccgtctcgg gctgcgcgcg atgctcgagc gtcacaaaga agagctcaac gtcgtcgctg
     7321 taaacgatat ggccgacttg cataccaacg ctcacctatt tcgttacgac tcaacctatg
     7381 gaatctatcc gggaagtgtc caggttggcg aaggccagct caagatcgaa ggatgggata
     7441 tcgccgtgct gtgcgagaaa gatccgtcgc ggctgccttg gcgcaagctg ggggtggaca
     7501 tcgttatcga atccaccggc ctctttacca gcggcgttca agcgcgagcg catttggaag
     7561 cgggagcaaa gaaagtgatt atcaccgcgc ccgcgacaga cgaagacatc accctggtgt
     7621 tgggagtcaa cgagtcggcc tacgaccctg acaaacacca catcgtttcg aatgcgtcct
     7681 gcacaaccaa ctgtctcgct ccgctcgcca aagtcttaca cgagaacttc ggcgtcgaac
     7741 gcggtctgat gacaaccaca cactcctaca ccaacgatca gcgcattctg gatttgatgc
     7801 acagcgatat gcgacgcgcc cgcgccggcg cggctaacat cgtgccgacg accaccggcg
     7861 ccgccaaagc catcggactg gtcttgcccg agctcaaggg caagcttcac ggtctgtcgc
     7921 tcagagtgcc gacatcgagt gtctctatcg tcgacctagt ggtcgatctg aaaaagccgg
     7981 catccgcagc ggcgattaac gagtcgctca agagctctgc cgatggtgat ctcgatggca
     8041 tcttggccta ttgcgatgag cccctggttt ccagcgattt ccgcggcaac tcggcctcct
     8101 ccatcataga tgggttatgc accgtccaac tggaggagaa attggcaaag atcttggcgt
     8161 ggtacgataa cgaatggggc tacagctgcc gcgtcggcga cctcgccgcc tttatgatcg
     8221 agcgagaact ataacttttt tgactttgtc tcgccaactt tgatcgctag tcgcgccttg
     8281 cgaaactttt actctcacgt ttgtgtcaga tatttcaccg gtagcgccac ttcctgcacc
     8341 gagccataga cactgcgcat gctgtaagcc cgaacgcccg gctttgctcc gatcactcga
     8401 tgaatctctg tgcctttgta gtgaatcgcg gcccagtcct cgatcgccac gccggcagaa
     8461 atctcgccac tggccagaaa ccggtgatac gatggccggc gctgcggttc tccgtcgtaa
     8521 tgcgggcaac agcttccggg aagaaaaccg agacagccaa gcggacgcag tccatccgag
     8581 aacgagtccg tcagcccttg ctcgaaccaa cagatcgctc ccgcgctcac tcccgtaagg
     8641 ataactcccg actgccacgc ctctcgaagc aattccggca cgccccaatc gcgccatagg
     8701 gcaagcaagc ttttggtatt gccgccaccg acaaaaatca catcttgatt tagcagcaac
     8761 gagcgcagat ccggcgtcct tttaaaaaac gacaacaccg aaggtcggca ttgcagtttc
     8821 aaaaatgccg catagaaact tgcaacataa tggtccggct cgccgctggc agtgggcaca
     8881 aagagcaccc gcggctcggc agctccggtt tgcgctaaga tgtaacgttc gagcgcaaga
     8941 ttctccgggt cgcgatagaa tccgccgccg ccgataggaa tgatctgtgg tgttccatcc
     9001 aacacgtgct tgactcctga ttggctctgt cttcaacccg gtgccgagct agtttaccta
     9061 gcttctcaat tcgcctcgag cccgctcggc aattttttcg tagcctgctt tgatccggtc
     9121 gagctcctct tcttcaagct cttccagatc cagcaacgca ttttgcgcac cgtgcgtggc
     9181 gcggatgagt tcatccaatt tcacctgcaa cgcctcagaa tcacgatttt gggtattttg
     9241 tatgagaaag accatcaaga aagtgatgat cgtggtcccg gtgttgataa ctagctgcca
     9301 ggtatcgcta aagccgaaca tcggacccat gatggcccag accacgacaa tcgaaacagc
     9361 aatcataaat gtcactggct tgccggtgat ctttgcagtt gatttcgcga atcgcgagaa
     9421 ccaagatctc gatcttgtcg gcctcatagg ttcctcctca cgtattgatt aaatcgtcat
     9481 gttcatatca catgcggtgg cgaaggcgac aattaagcga tagctaatcc gagcgcgctt
     9541 cccggcgcgc tgataagcca gattcaatct ggacattccc gaatcgctgg agtaatcggg
     9601 gatgagatca ccgattcgtg atggaaagga acctatatgg ccacattaat caccggcttg
     9661 ggcttggttg gaacttccta cgctcaactc gcgctcaagc gcggcgaaag catcgttttc
     9721 tacgatatcg cgccaaggaa agacttcctg gcgaataaat tggggacggc gaacgtgacg
     9781 gtagtgcagc gcgatgtccg tgacttgccg gcgctgatcg aaacgatcct gaaaaacaaa
     9841 atcgacacgg tcatccacac agcgggactg atcggcggca aagtcgcgca gcccgtctat
     9901 accggcatgc agatcaatgt aatggggacc gtcaatgtcg ccgaagcggt gcgcttgacg
     9961 ggggtcaaac ggctggtgca gatcagcacg ttcggcgttt acgaccgccg cggcggcgag
    10021 cccggcccga tcgacgaaag ctttcatcgc ggacccggcg aggcgtatgg caattccaaa
    10081 gtcgccaagg aactgatggt agaagcgtat caacggctgt acggctttga gctgatcatt
    10141 ttaagactcg ccaatgtcta tggcgtcggc cactttgccg gcggttccgg cggcggcgag
    10201 atggtgcaga acatgctgca gactggaatt cgcggcggtg tagtaaaggt tccgcaagaa
    10261 gtggcgcgcg acttcgaata catttactac aaagacctgg gccgagctct cgacaaggca
    10321 gcgatcacac cgctatcgtc gccggtaaca cttaatatcg gcactggcgt agtcattaaa
    10381 ttcgacgatt tggtcgccac agccgagaaa ctcctgccca atctgcaggt ggaactgata
    10441 ccggggcaga aacctcgttc cgccaagcag cccatggtga tcaccagagc caaacaattc
    10501 ctggggtggg aaccagagta tgatttggaa cgcggattca aagactacat cgaggagctc
    10561 aaggcgttga gctgataaaa aggcatcgca gctcccgtag aacccgatgc ctcagagttt
    10621 gagctcgcct cgtttgccgc tcttggcgct ttcgtagcgc aggtcattgc catcagtcgt
    10681 gatgatgatg gcgccgtctt cttcggtgcg gagaatgtct gcgccggcag cgcgataacg
    10741 atccgcaagc tcttgcttct cagggcgcac gtttctggtg gttcctgaaa ccaccgctag
    10801 cctgggttgc acggccgaaa taaattccgg ggtgctcgcc gaagcgctgc catgccgcgg
    10861 cactttcaca accgcactgt tgatccggac cggtatttgc gcaagaaact gctcgtcccg
    10921 cttgtcgatg tctccggcga aaagcaaccg cgctttacca tagtggagct gtactaccac
    10981 cgaaacatcg tcgttccgat cggccggcgg ataaaggaaa cacagcttca cccgttcgat
    11041 tgagcgacat ggtgcttcgt tggtcagact gacccgttga atcttcgacc gatccaagat
    11101 gtcatccagc tcctcgaatc gtcgcgttcg tcctttggcg gcgccggacc agaattcgtc
    11161 gggggtaaac tctctgacga tcgatcccat tccgccatag tgatcgatgc gcgggtggct
    11221 gaccagcaga taatcgacct tcagaatctt gcgagagcgt agatacggac cgacaatcgc
    11281 ctcgccggta tcaaactcgc cagtagctgt gcctccggcg tcgatcagca aagttttagc
    11341 gccgggaaac tcgataacga ctgcatctcc ctggccgaca ttcagataag tcactctgag
    11401 ttcttgccgc gtccaacgtt cgcgggacca gtaaacaaca tccgcgagca acattgacgc
    11461 gcttaaagcg accgcaatga cagcgcggcg gcttttcctg agccagagta cagaagcaat
    11521 caatagatac acggcgccaa tctcccacag gtccggcgcc ggtactgaga tatgagcaaa
    11581 cggaagtctg gcgaaccatt ccaccagcag tcccgagaac cacagcaccc actcgagaac
    11641 cgagactaaa ggagtgacca atcccggcca tgcaagagag atgaaactaa tcccaaggcc
    11701 gagcggaaca acgacaaagc caaccaacgg cacgactatc ggattcgaga cgaatccggc
    11761 caacgatagg tgaccgaaat agtgcgcgat caacggaccg gtgccaatgg tcgccaggag
    11821 cggcacagcc aagtgcattc caatcgagcg cagctttggt tttatccggc ttcgctcctg
    11881 aggcaagcgg tctttctcgg gcgcggcgac cgtcctaaaa acctgacgca ttccccaggc
    11941 gatcgaaaaa actgcgagaa acgaaagctg aaaggagatg tcggcgatga cgccgggcca
    12001 aaccagcccg attagcaacg ccgcaaacgt cagactggtt aaaacctctt cctctcgatc
    12061 caacaagaca gccaattcat aaactccgat catgatcgcc gatcgcaccg tcggcaccat
    12121 ggcaccggcg agcgcggtgt agaaaaccac cgcaatgaac gaacaaaagg tcgcgatttt
    12181 gatcaaatta caccgcaaca gcaaataaac attgaaagaa ccggcccagc gcacgagcca
    12241 aaacaccacc aaaccgagca tcgccacatg caggcctgag atggatagaa catgattgac
    12301 tcccgcggcg gtaaagttgg cgcgcatctt cttggaaatt tctcccatat cgccgaccac
    12361 caaggccttc atcagagcgg cggattcggg ggaaaagtgg gtttcaatgt agcggcgaat
    12421 ttttcgccgc agattttcca ccgcgccgcg ccatcgatcg ggggcgcgtt ccaccagctc
    12481 gatcttttcg tcggattcta aaaatgcgat ggcataaatc tggcggcgcg atagataggt
    12541 agcgtaattg aaaccgccgg gattgccgct gtctttgggc acagtcggcc gaatctgcgc
    12601 gcggatgcga tcgccgtagt gccactcacg gcgaagctgc cggacagtca acagcagatc
    12661 gcccgagatc tcctcggcgc ccgtcggctg ccatattcgt tccgcgcgca aataccagcg
    12721 gctccgggtc ggcagttttt ccggctcatg tagcagcact ccttctaaat acaaatcttt
    12781 gttctcgtcc attatcgagc gaaggtgatt cgccggaaaa gttggaaaca acaacccgtg
    12841 atgacggaag taacccagcg caaacgtcga tcccgctagc aatgcgagca gcgcccaacg
    12901 tcgatagttg accgtgctcg cattgcaatg actcgttaga tcgcggccga cgatcacgcc
    12961 agccagccat ctgagcttag caagcgggtt gataaggagg agcgagggaa ccaacaataa
    13021 cgacaggagg tcgccggaga gaacgaatgg caaggcggtc gccgattggc ccaacagcag
    13081 cgagccggtc agcgcaaaca gccaagttga tgggtgaatg gctttggaaa ccgttggcgc
    13141 gatttgctgg gaaactagcg ccatcaaccc gctctcactg cggaggcgga gccgggtgaa
    13201 acacttgcca gagttgatga gcgagcttca gattcatgct tggaacctgc attaactcat
    13261 ccaaggtcgc ctgttcaacc cccttgacgc tgccaaatgc gcgcagcaaa gcgcgccggc
    13321 gcgccgcccc gatgccggga atctggtcca aggccgagga gagggtttgc tgagaacgga
    13381 gtttcttgtg gtgcgtaatc gcgaagcggt gcgcctcatc gcgcacccgc tgcaagagaa
    13441 acaaggcatt cgaattacgt ctcaacgtca ccggattggt ctgtcccggc agaaagacgc
    13501 gctcctccga tcgctcgatc tcggcgctgc gaggcgcgga ttgcacgcgc attttggcca
    13561 gaccgacgac gtcgatacct tcgacgccga cttcgcgcat ggccgccagc gccatcgcca
    13621 attgcccctt gccaccgtcg accaccacca ggttgggcat cacttcttca cgttggcccc
    13681 gagtaaagcg ccgtttgaga acttccaaca tcatgccgaa atcgtcgccg ccaccggacg
    13741 gatcgatggt gcgaattcga taatgacgat agtgcgcctt gtctggttcg ccgccgatga
    13801 acgtaacctg agacccgacc gcataggcgc cgtgaatcat cgaaatgtcg aagcactcga
    13861 tgcgctgcgg gtagcgcttc aagcgcaggt gctcctggag ctcttgaagc atgttctcac
    13921 gggctttctc ctgatcgtga cgttcggtaa agctttgccg ggcattctgc tgcgccattt
    13981 cgaccagctt gcgcttgtca cccttctggg gcacgtgaat gctcacgttc ttgcctttgc
    14041 gctcgctcag atactcggcc cgggtatcct gatcttccag ttccacgggc agcaagattt
    14101 cgtctggcag gaagcgttgt ccttggtaga attgcgtcag cagcgaaccc atgatttcct
    14161 cgtcggagaa agccaaatct tccaacgcat acgcttgcgt tccggtcagt ttcccttgtc
    14221 gaacgaagag cacctgggcc tcgatataac cgccttcgcg gtaaaggccg aagatgtcct
    14281 gatcggcacc ccaatgggaa accatccgct gtttttcgag agttttctcg accgcctgga
    14341 tttgatcgcg gatcttagcc gccgattcaa attccatagc cccggctttg gccgccatgc
    14401 gctgccgcag ttccgccagc aattcgtgtt gctttccttc gataaacaga attgcctggc
    14461 gaacctgctg gcggtactct tccggatcga ccggcagcac gcacggcgcc aagcatcgct
    14521 ttatttcgta ttgtaagcag ggccggctgc gattcttgaa gttgtggtcc gtgcaattac
    14581 gcaaaagaaa atgcttctcg atgatatcga cagtttcacg cgccgccacc gcggacgaga
    14641 acggaccgaa gtagcggctg ccgtccttga cgatcttgcg ggtgctaaaa attcgcggcc
    14701 atacgtccgg ggtagtcacc ttgatgctta aataactctt atcgtccttg agacgaatgt
    14761 tgtaccgcgg tttgtattgc ttgatgagat tgttttcaag aatcagagct tccttgtcat
    14821 tgctggtgac aagagtctcg agattttcga cccgcctgac taagaactcg atttgtgaac
    14881 ggccatcatt gcgattgaag tacgatctta cccgcgcgcg caagtccttg gccttaccga
    14941 catagatcac ctttcctgcg ccgtcgcgca tcagatagac ccccggacga gacggaaggt
    15001 tggtcaactt ttcatccacg gaaagcatcg ctatgagtat atcagccggg tacggcccgc
    15061 ggcgacaagc gcgcccagtc cagccattta gctcgacacc cacgtctcat tgccgtcagc
    15121 agaagtcaat gcaagcagaa tctttgcacc gctgagatga attgatgccg gcagtgactc
    15181 tataactctt gggctgctga cagcgcgccg tcgcgcagct gcagctcgcg gaattccagc
    15241 tcacggatct gatcccgcag ctccgccgcc ctctcgaatt cgagcttgcc ggatgcctgc
    15301 ttcatttcct ttttgagttg cttgattttt cgggtgagat cggcgggagc ataacgtttg
    15361 gagccatcag cagcgagcgg gatttcgacg taatcggcct catagatttt caccagcggc
    15421 gcgcccagag cctttatgat cgtctgcggg gtgatgccgt tttcgctatt aaaggttgtc
    15481 tgtagactgc gccgccggtt ggtttcatca agagctcgcc tcatcgaatc cgtcatagaa
    15541 tccgcgtaaa gaataacctc gccatgaatg ttacgagcgg ccctgccgat ggtctggata
    15601 agtgagcgct cggagcgcag aaatccttcc ttgtcggcat cgagtatggc gaccagtgag
    15661 acctccggca gatcgagtcc ttcgcgcaac aggttgatgc caaccagcac atcaaaaact
    15721 cccatgcgca gttgccggat gatctcgaca cgctcgatcg tttcaatgtc cgaatggaga
    15781 tagcgcacct taacgcctag atcttgatag tagtcggtca gatcttcagc catgcgcttg
    15841 gtcaacgtcg tgacgagaac ccgttccttg cgctccactc gtttgcggac ttccgccaag
    15901 agatcgtcaa cctggttacg ggccggtctg acggtcacct ccggatcgat caatccggtg
    15961 ggccggatca cttgttcgac aacgacacca ttggcctttt ccatttcgaa gttcgacggc
    16021 gtagccgaga cataaataac ctgattcaga cagccttcga actcgccgaa attcaaaggc
    16081 cggttatcca acgccgaagg caaccgaaag ccgtattcga ccagagtctc tttgcgcgag
    16141 cggtcgccgc gatacatgcc gcccacctgc ggcacggtca cgtgactctc gtcaatcacc
    16201 agcaaaaaat cgccgggaaa atagctgagc aatgtcggcg gcggctcacc cggcctccgg
    16261 ccggtcagat gacgcgagta gttttcgatc cccggacaga aacccatttc ctccagcagc
    16321 tcgagatcgt ataacgtgcg ctgttccagc cgttgcgcct cgagcaattt gttttgatcg
    16381 cgcaactcct gcaaccgctc tttgagctcc acacggatat cgcgggtcgc ctgtttcatg
    16441 cggctttccg tggtcgcgta gtggctcgcg ggataaacca tcgctttatc cacccgccgt
    16501 tacactttgc cgcgcagcgg atcgatttca tagatgcctt ccacgg
//
LOCUS       DASUVS010000082        18893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_684876, whole genome shotgun sequence.
ACCESSION   DASUVS010000082 DASUVS010000000
VERSION     DASUVS010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 18893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18893
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684876"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VF182_04765"
     CDS             complement(<1..568)
                     /locus_tag="VF182_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX6436419.1"
                     /translation="MIELPAACFYTPALEKIMSSLKIATWNVNGIRAREAQVLEWVER
                     ERPDVVCLQELKSTVAQVPAALCELQGYRCYWHGARAYSGVGLHIRRDAFPGEPLFFH
                     PSFDFETRVVAAQMDNLVVVSAYVPNGGKDFAAKMQFLRDMENYVCEVHASGALLVFC
                     GDLNIARTERDVHPKERNPGIIGQRPEER"
     gene            790..1461
                     /locus_tag="VF182_04770"
     CDS             790..1461
                     /locus_tag="VF182_04770"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEX6436420.1"
                     /translation="MIARIGYLILIDTGALMLVKDEHMQTVYIDMDDVLCQTAQHFLA
                     ILERDFGKKFAFEQLTDFDVGQACELTAEEREVLYRIVHDGEELLSIPPIPGAVDVLQ
                     QWSAAGYEIAIVTGRPPGTYEPSVQWLKKQRVPYDSILIVNKYGRFAPDGSRCISLEA
                     LINHSFSWAVEDSPEMAMYLASQMKIPVALLDRPWNRKDTNHELVSRHYDWIEISRAL
                     PPNNR"
     gene            complement(1533..3008)
                     /locus_tag="VF182_04775"
     CDS             complement(1533..3008)
                     /locus_tag="VF182_04775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX6436421.1"
                     /translation="MQAVIQIKDNLMNFRKVTSKSNAAWALMILAAGCSVGPNYKAPE
                     ITVPASWNEAQQSDFDTGPAPLLDWWTEFNDPLLNNLVQRAVHLNLDIRLAESRIRES
                     RALRAVTAADAWPGFDAAGSYRRSRSSENAFVSGGGGEAFLGGSGAGDLFRAGFDASW
                     EVDIFGGVRRRVEAADANVAASVEERRNALVTLLGDVARNYIELRGFQRRLAVARSNL
                     QAQQETLDLTRVRFEAGLSSDFEVAQSEGQVNTTAAQIPALESALKGSVHRLDVLLGQ
                     QPGALWAELSNEAPVPALPPQAHVGLPAELLRRRPDIRRAERVLAAATAQVGAATADL
                     YPRLSLTGAFGFQSVNASDLISSPSRFWSIGPTIMWPVFDAGRIRANIAVRNAQQEQA
                     LTLYEKTVLTAFEDVENSLVNYAKEQTRYRALMDAVVANRRAVQMANELYTRGLVDFL
                     NVLETQRSLYASESELAQSETVMASNLVALYKALGGGWETD"
     gene            complement(3019..4137)
                     /locus_tag="VF182_04780"
     CDS             complement(3019..4137)
                     /locus_tag="VF182_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6436422.1"
                     /translation="MLDALYRILALTRKELLAILKDPRSRFSLILPPILQCLIYGYVA
                     TYDLNHVPYAAFDHDRSAASRQLLARIDGSGVFQRVANLDRELEVKNIIDQARALLVV
                     QIDRDFERRLLSGMPATVQVIADGRNSNTAGTAIGYVDAIVNAFNADWRSRHGQGGAP
                     IRITTRAWYNPNLETRWHMIPALIGTLTLIQTLLLTGLSVAREREQGTFDQLLVTPFR
                     PAEIMAGKALPAVLVGLVQATMVLLVAQLWFQIPFAGSFVTLYVGLGVFLLAAIGIGL
                     LLSSLAATMQQAMLFSFLLIMPFSLLSGLTTPISSMPMVLQYLTLLNPLRYAIDIAHR
                     VYLEGVGLNLLLSELWPLALMAAITLSAASWMFRHRLT"
     gene            complement(4144..5268)
                     /locus_tag="VF182_04785"
     CDS             complement(4144..5268)
                     /locus_tag="VF182_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6436423.1"
                     /translation="MTTGFNSRRIGALVRKEAKQMLRDPSSIAIGIVMPVLLILLFGY
                     GLSLDVTRVPVAVVLEDPSPATTELAAGFQLSPYFEPVLMTSMANAEELMLARKVDGI
                     IRIRPDFARTLTAGGAEVQILVHGVDANRARIIQNYAQGAIGRWSARRAAQGIEGSAS
                     PATVQSRMWFNEANESRYFLVPGLIVLIMTLIGALLTTLVMAREWERGTLEPLFVTPV
                     RTDEILLGKTIPYFILGMIGLVLCLLAAKFLFAVPFRGSVTVLTGASMLYLLVALGIG
                     LLISSTVKSQFVASQITLLVSFLPAVMLSGFLFDLRSMPDLVRTITYLLPARYYVALL
                     QTIFLAGDVWPVIIPNALVLTGMAALLVFLTRKVTRKRLA"
     gene            complement(5265..7196)
                     /locus_tag="VF182_04790"
     CDS             complement(5265..7196)
                     /locus_tag="VF182_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6436424.1"
                     /translation="MNDSDNPITLVGRDLRKTFRRDSGEKVDALNGVSIDVTHGTLTA
                     LVGPDGAGKTTLIRLAAGLMLPDGGDLKILGIDVAAEPQRVQDRIGYMPQRFGLYEDL
                     SVQENLDLYADLHGVSMKERRQRYASLMEMTALGPFTQRLAARLSGGMKQKLGLACTL
                     VRAPELLLLDEPTVGVDPLSRRELWNIILQLVSQQNLTVLVSTSYLDEAELCGHVAVM
                     HQGNILAQGHPEAISSLAQGRVFLAEPPAGQKAREYQARLLDKSEVLDAVPQGGRVRI
                     VTEKLKDESGKLKETVRSGETGDKETERQGDPDNFDAIAISAEPRFEDGFMVLLKSKK
                     ETRRQGEKDGQKMDLETTRQANKQPVSSASLSLSLPVSLSPNSPVSRPPEAAVVQVQN
                     LVRRFGKFIAVDHVTFEAQRGEIFGLLGPNGAGKTTTFRMLCGLLPASEGTLRVAGVD
                     LRRARASARQHIGYVAQKFSLYGQLSVSENLEFFARAYNLRGMQKDCRIDWALQQFEL
                     STLANLPSGNLPGGFKQRLAMAAALLHEPETLFLDEPTSGADPLARRDFWRRITALSQ
                     EGVTVVITTHFMEEAEYCDRIAIMDGGKILAQGTPAAIRSQASGARKGESTMEDAFIA
                     VVEEARTKPVQTGSARGAA"
     gene            complement(7186..8211)
                     /locus_tag="VF182_04795"
     CDS             complement(7186..8211)
                     /locus_tag="VF182_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX6436425.1"
                     /translation="MKRRLLIAGLILLSLIAAGVAWRLIQREGDPTPLALYGDIDLRQ
                     VQLSFNNSERIATVLVQEGDRVRKGQLLARLDTARLEPQLAQAEAQAAAQLQVVKRLR
                     SGSRPEEIAQARANLQAAQADLTNARQQYERWRSAAEISAGRAVRQQDVDNARTAVQV
                     AEAKLAVVQKTLDLTIAGPRREEISENEARLRAFEAQARVLRQQLEDAQLIAPVDAVI
                     RTRLMEPGEMASPQRPVFSLAITDPKWVRSFISEPDLGKIKSGMAATIEVDSFAGRRF
                     DGWIGFISPVAEFTPKTVQTEELRTSLVYEVRVFVKDPADELRLGMPASVYLQVTNRD
                     PELRDER"
     gene            complement(8246..8875)
                     /locus_tag="VF182_04800"
     CDS             complement(8246..8875)
                     /locus_tag="VF182_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6436426.1"
                     /translation="MPSSRSRKDSLIQPRHDAAARIIAVARQHFFAHGFRSVTMDDLA
                     GELGMSKKTLYAFFPTKTDLLQAVLLDKFRRVEADLQLIATALSNDMLDSLRQLLACL
                     QRHTEEIRPPFVRDIRREAPQMFELVQTRRRQIIERYFGSLLEGCRKAGIVRKDISTR
                     LIIEILLGATEAIMNPIRMAELDLTPKRGYSTIINVVFEGVLTEKGRQR"
     gene            complement(9029..12175)
                     /locus_tag="VF182_04805"
     CDS             complement(9029..12175)
                     /locus_tag="VF182_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX6436427.1"
                     /translation="MSFSELFIRRPVMTMVLTVSVVLFGIVSYLHLPVNDLPAVDYPV
                     IQVQANYPGASPDTVANSIATPLERQFMQINGLELVISKSTQGHTSLTLQFALDKSVD
                     VAATDVQTAISQATGNLPVDLPSPPTYSKTNPNDQPIMYIALTSDSVTVGQLYDYAST
                     QVGQRISILPGVSRVNVFGAKSAVRIKADPSAMWARGISVEDLAEAIRNGTSTIGAGQ
                     FDGVGGTLLLRPQGQLDDAKEYSDLIISSKNGSPVYLRDVAQVQNSVQDERINMRFWV
                     RGYDPPAATVIVAVNRKAGANAVEVAQSVRQLLPLISTELPGSVRVTPIYDRSQTIIH
                     SIIDVQLTLAIAFALVVIVIFVFLGRATDTLIPAVALPLSLLITFVIMDLLEFSLDNL
                     SLMALTLAIGFLVDDAIVFLENTVRRMEHGQKSLEATIASAREISFTIISMTISLAAV
                     FIPLVFMPGLVGRIFREFAVTIVVAIIASGLVSLTLTPLMCARMLSDRGKGSKQTWME
                     RKMSAWEKSFLERYSRALWWFLRFRWVSALIWVVCLAGTVWLFTLVPKAFLPPGDSSF
                     IFGLMIGREGASPGQMHELQNQADEILRADPGVRATFTMTGNGQFLASNQGLLLAFLE
                     EPGNRAPIEQVAGGLMGKLAMVPGVMPFMRPRPVLEISTGATNQNQGQYAFSLSGVNP
                     KQVYEVAGKLMERLRPFPGFASLSSDYFNNTPSLDIEIKREQARLHGVSEARILQLLR
                     SAYAQNYLYLIKKPEDQYQVILEAEDAARSEAQDLSLLHIKSDNGKNLVPLNTLVTWK
                     ESLGPQAVNHLNQFTSVTLFFNLKPGVAVGEATDFINKAAAEIVPPTVRASLQGEAQT
                     FQDTVRHLTVLMALAVFVMYVILAILYESYLHPLTVLSTLPTALVGGLLTLWLFGEQA
                     SLYAFVGMFMLMGIVKKNGILIVDFAIHRVVAGEPADQAIHDASLDRFRPILMTTLAA
                     VFGALPIALGIGADGASRRSLGLVIVGGLVVSQFITLFITPVIYLYLEEFQEKVLDRT
                     SFFRSQRGARTPELSAER"
     gene            complement(12172..13302)
                     /locus_tag="VF182_04810"
     CDS             complement(12172..13302)
                     /locus_tag="VF182_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX6436428.1"
                     /translation="MLQILGFLSVTLLAGCDRKAPAALERPPAPVSVATAVSQDVPIY
                     IDAVGKIVAREVVSIQPQVSGRITKIHFADGANVKIGDVLFTIDPRPYQAQLNQAEAN
                     VAQAQAALNLAKTNFARVESVSDPRAVSRQDFDSKKNAVEVAEAQLRQNRAAVETARL
                     NLEYCTIRSPIEGRAGQRLVDLGNVVTPGNSPLLVIQRLDPIYADFTIPESDLTEVQR
                     NMARRSLRAEVRLDDESTRAREGKLTFLDNSVQEGTGSVKLRATVENKDRLFWPGRFA
                     RIRLILDTRHSAVLVPADAPQLSAKGSFVYVVKQDESAELRSVTVGQRQGDLVVIAQG
                     LKEGERVVVNGQLGVTPGGKVQIAAPGSEKNPAAASRSGSKP"
     gene            complement(13352..13783)
                     /locus_tag="VF182_04815"
     CDS             complement(13352..13783)
                     /locus_tag="VF182_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436429.1"
                     /translation="MEPMKTTIEEESREKSFGAEDRSSPLFQPDTLMSDEYFANYRRR
                     IPLEPEKALLLAVLEDGVRTFQDNIFAESGKKRALLDEAREWLFTDGVEHVFSFNSVC
                     SSLGLEPGYIRRGLKRWEEHVRAATRKKQRVPQAAPERLVA"
     gene            complement(13828..14517)
                     /locus_tag="VF182_04820"
     CDS             complement(13828..14517)
                     /locus_tag="VF182_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6436430.1"
                     /translation="MGNVARLSADQRKRAIVDAVRDVFAEKGFDGTTTRELAKAAGVS
                     EALLYKHFPSKESLYAAMLDACAKGPTFAQFKRILALEPSTSTLVLMVYFTISHYVLQ
                     RPGDPHKAALNCLLVRSLLEDGALVRLTHKKFARAWVAKFEACVNEAVQTGDLGEIPI
                     RRDLRVWFVHHIAFSLMLHLHPKVPAIDYNVSKDALVEQATWFALRGVGLGEESIKRH
                     YNSKALSLLGD"
     gene            14900..15709
                     /locus_tag="VF182_04825"
     CDS             14900..15709
                     /locus_tag="VF182_04825"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6436431.1"
                     /translation="MALRINDCEYYAIDAHSHLGSRQTPLGHGVASFLGDDLVRNLDE
                     AGLDCAVAFPLGASYTDYSDANQIMAEEMAKHPKRIISFCRINPNFGPQASAKSLEHC
                     LGKLKLKGIKLHPEIEFFDPNEAELMEPIYAAARRYHVPIIFHTGMSSKASPGVIAEL
                     AAQYRDVPVILGHMGVSEYVKLAVAVARQNENIFLETSVVGWMPLLLEAFRGVGTSKI
                     LFGSDHPYNPLSMEVEKISRHVTQAAKLNQADLKKVFSDNFLSLLKWEQRV"
     gene            15765..16763
                     /locus_tag="VF182_04830"
     CDS             15765..16763
                     /locus_tag="VF182_04830"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436432.1"
                     /translation="MRSFSRIVLVVLIAVAVLTGQTDESLAQERIRIAWAGVSPANSP
                     IWVVQERGLLKKHGLNGEVISISASPIALQALLAGEVDVIVTSVTTLALSRLAGADIV
                     MILGMVPTFLDHIISDPSVTTLEQIKGKTGGVNRLGSTSDLGLRLALRRKGIDPEKDV
                     KIITAGDNPARLAAISRGIIQFSIMPEPFVREAEKLGFKDLFDIQSLKIPFWWNAVLA
                     KESAVKTNRAVLTKFSRAMMEAIHYIKTDKEGTKAIYSKNLKLTDPEGLERAYQAYVS
                     VFPENLLPSPDGVKTMLDDLAPRNPKAAAADPKTFVDMSLVQEIEASGFIKQLYKR"
     gene            16896..17909
                     /locus_tag="VF182_04835"
     CDS             16896..17909
                     /locus_tag="VF182_04835"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436433.1"
                     /translation="MRLARISVRGCASAGRLVALLSVFYILTGSLSARAAEMPLVRAN
                     YAAISGAFAPLWIATEKGLFTKYGLNVDLRYIAPATATQALLAKNLDIINPGGEIVEA
                     GLNGEPVVFIAGILNRAVMSIYAKPEINSMSDLKGKVLGVTVPGATTDFAARVLLQQS
                     GLAPGKDVKLLYLRGMPEILAGINQGNAVAGIFTAPTTLKARAAGLKELVNVTEQNIP
                     MIHAALATTRDYLKINHDPTQQFLRAYLEGIKIALTDAEFTKQMIGKYTKTTDAKDLE
                     SSYQAFLPAWERLPIVPAAAVQTMLNFASHPGAKVAKPESLIDNSILMELGKSGFVDK
                     LYK"
     gene            complement(17984..18346)
                     /locus_tag="VF182_04840"
     CDS             complement(17984..18346)
                     /locus_tag="VF182_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6436434.1"
                     /translation="MKKVLIADDYQDMRELLARQIESLGFLPILAENGKDVLEKAMRE
                     KPRLILLDMIMPIMDGWEAVRSLRANPETKDIPILAATALFHQSDLNTCIEQGCNGYI
                     NKPFTIVQLKKKIEELIR"
     gene            complement(18635..>18893)
                     /locus_tag="VF182_04845"
     CDS             complement(18635..>18893)
                     /locus_tag="VF182_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436435.1"
                     /translation="CGIRVALSRVVIPQHRIKQRKGRVMRPLKNLRNERGQGLMEYTL
                     IIFLVALVFWGGIKSTNIGDELAGSWDKVIDCITTPFSCGS"
BASE COUNT         4424 a         5268 c         5045 g         4156 t
ORIGIN      
        1 ccctttcttc cggcctttgg ccgatgatcc cggggtttcg ctccttcggg tgcacatcgc
       61 gctccgttcg cgcaatgttg agatcgccgc agaagaccag cagcgcgccg ctggcgtgaa
      121 cttcgcaaac ataattttcc atatcgcgca ggaactgcat ttttgccgca aagtctttgc
      181 cgccgttggg aacataggcc gagaccacca ccagattgtc catttgcgct gcgacgacac
      241 gcgtttcgaa atcaaaagaa ggatgaaaga aaagcggctc cccgggaaaa gcgtcgcggc
      301 gtatgtgcaa accgactccg gagtaagcgc gcgcgccgtg ccagtagcat cgatatccct
      361 gcagctcgca taatgcggct ggaacctgcg ccacggtgga cttcaattcc tgaagacaga
      421 caacgtccgg gcgctcgcgc tccacccatt cgagcacctg cgcttcgcgt gcgcgaatgc
      481 cgttgacgtt ccaagtagca atcttcaacg aagacataat tttctccagt gcaggagtat
      541 agaaacaggc cgccggaagt tcaatcatga taaactcgga ttttgaagta gaagattgtg
      601 tgggtgtacc gatcgagcgg cgagcgtgaa aggagcaaag cctcaggtca ttgtcttcgc
      661 tccgaaaggc ttgccgcttg atcgttgcgt cactttgaac tgcgcaaatt gggataaaag
      721 gagctctgaa aacgccagac tgccgtcaag aagattgggc gatccagact cggcacatga
      781 aagcgatggt tgattgcacg tataggttac ttgattctaa tcgataccgg agcactgatg
      841 ttggtgaagg atgaacacat gcaaaccgtt tacatcgaca tggatgatgt cttgtgccag
      901 acggcgcagc actttctagc gatattggaa agagatttcg ggaaaaaatt tgcctttgag
      961 caactcaccg atttcgatgt cggccaggct tgcgagctta ccgcagagga aagggaggtg
     1021 ttgtaccgga tcgtgcatga cggtgaggag ctgttaagca tcccgccgat cccaggagcg
     1081 gtcgacgtct tgcagcaatg gagcgctgcc ggctatgaga ttgccatcgt tacgggccgc
     1141 ccaccgggta cctatgagcc ttcggtgcaa tggctaaaaa aacaacgggt gccctatgac
     1201 agcatattga tcgtcaataa atatggacgg ttcgcgcccg atggcagccg ctgcatctcc
     1261 ctcgaagcgc tgatcaatca tagcttctct tgggccgtgg aagactcgcc ggaaatggct
     1321 atgtatttgg catcacagat gaagattcct gttgctttgc tggatcgccc gtggaaccgg
     1381 aaagatacga atcatgagct ggtaagcagg cactatgatt ggatcgagat ttcacgggca
     1441 ctgccgccga acaatcgata atcgtcgatc aaagcgaggt tcacggaaaa tcatccgttt
     1501 cagctttccg ctttcatcct ttgtactttt ctttagtccg tctcccagcc gccgcccagc
     1561 gctttataga gcgcaacgag attcgaagcc atgaccgttt cgctctgggc gagctcgctt
     1621 tccgaggcat acagcgagcg ctgcgtttcc agcacattga ggaaatcgac caggcctctg
     1681 gtataaagct cgttggccat ctgcaccgcg cgccggttgg caaccaccgc gtccatcaaa
     1741 gcgcgatacc gggtttgttc cttggcatag ttcaccagag aattttccac atcctcgaac
     1801 gcggtcagaa cggttttctc atacaaagtt aaggcttgct cctgctgcgc gttgcgcacc
     1861 gcgatattgg cgcgaatccg gccggcatcg aagaccggcc acatgatcgt tggtcctatg
     1921 gaccagaagc ggctcggact gctgatcaaa tcgctggcgt tgacgctttg aaaaccgaaa
     1981 gctccggtta atgacaatcg cggatacaga tcagccgttg cggcgccgac ttgcgcggtc
     2041 gcagcggcta acactcgctc ggcccgccgg atatccggcc gccgcctgag aagctcagca
     2101 ggcaagccga catgcgcctg cggcggcaat gcaggaaccg gcgcttcatt gctgagttcc
     2161 gcccacagag caccgggttg ttggccgagc agcacatcga ggcggtgcac gcttcccttg
     2221 agcgcgcttt cgagcgctgg gatctgcgct gcggtcgtat tgacctggcc ttcggattgc
     2281 gccacctcga aatcgctaga cagtcccgct tcaaagcgca ctctcgtcag atccaacgtt
     2341 tcttgctgtg cctgaagatt ggatcgcgcc accgccagcc ggcgttggaa gccacgcaat
     2401 tcgatgtaat ttcttgccac gtcgcccagt aaggtcacta acgcattgcg acgctcctcg
     2461 accgacgccg caacgttggc atccgccgct tccacccttc gccgcacgcc gccaaagata
     2521 tcgacttccc aactggcatc gaagccggcc ctgaagagat cgcctgcgcc actgccgcct
     2581 aaaaaggcct cgccgcctcc gccggaaaca aacgcgttct cactcgagcg gcttcgcctg
     2641 taagaacccg cagcgtcgaa acccggccat gcatccgctg ccgtgactgc cctgagtgcc
     2701 cgcgactcac ggatgcgcga ctcagccaga cgtatatcga gatttaagtg caccgctcgc
     2761 tgcaccaggt tattgagcaa cggatcgtta aactccgtcc accaatccaa cagtggcgca
     2821 ggcccggtgt cgaagtcaga ttgctgcgct tcattccagc ttgccgggac cgtgatctcg
     2881 ggcgctttat aatttgggcc gacggagcat cccgccgcca gaatcatcag tgcccaggca
     2941 gcattagatt tacttgtcac ttttctgaag ttcattagat tgtccttgat ctgaattact
     3001 gcttgcatgt ctctctcttt aggttagccg atgacgaaac atccatgagg ctgccgaaag
     3061 cgtgatcgcg gccatgagcg ccagcggcca aagctcagat agtaataaat tcagacctac
     3121 tccctcgaga tagacccgat gagcaatatc gatggcatat cgcagcgggt ttagaagggt
     3181 caggtactgc agaaccatcg gcatactact gatcggagtg gtcagccccg acaacaatga
     3241 aaacggcatg atcagaagaa aagaaaatag catcgcctgc tgcatggtcg cggccagcga
     3301 tgatagcaac agcccgatac caatcgccgc aagcagaaaa acgccaagac ccacataaag
     3361 cgtaacaaac gagccggcaa acgggatttg aaaccagagc tgcgccacca gaagaaccat
     3421 agtggcctga accaatccaa cgaggactgc ggggagcgcc ttgcccgcca tgatctcggc
     3481 cggccggaat ggtgtcacca gcaactgatc gaaggtcccc tgctcgcgct cacgcgccac
     3541 cgacaagccg gttagcagca atgtctggat caaggtcaac gtgccgatga gagctggaat
     3601 catgtgccag cgcgtttcga gattggggtt gtaccaagcc cgggtggtga tccggatcgg
     3661 cgctccgcct tggccgtggc gagagcgcca atcggcgttg aacgcgttga caatggcatc
     3721 aacgtagcct attgccgtgc cggcggtatt ggaattgcgc ccgtcagcaa tgacttgcac
     3781 ggtggccggc atacctgaga gcaagcgccg ctcgaaatcg cggtcgattt ggacgaccag
     3841 caaagcgcgc gcctgatcga tgatattctt aacctcaagc tcgcgatcga gattcgccac
     3901 ccgctggaaa actcccgagc cgtctattct tgccagcagt tgccgcgatg ccgcgctgcg
     3961 gtcgtgatcg aacgcggcat agggaacatg atttaagtca taagtcgcca catagccgta
     4021 aatcaggcac tgcaaaatcg gtggcagaat aaggctaaat cggctccgtg gatctttgag
     4081 gatcgccaac aactccttgc gggtcagagc gagtatgcgg taaagggcgt caagcatatt
     4141 tttttaagca agtcgttttc gagtcacctt ccgcgtaaga aaaaccaaca gcgcggccat
     4201 gccggttagc accagagcat tgggaataat taccggccaa acatcaccgg caagaaaaat
     4261 cgtctgcagc aaagcgacgt aataccgcgc cggcagcagg taagtaatcg tccggaccaa
     4321 atccggcatg ctgcgcaggt cgaacagaaa tccggacagc atgactgccg gaagaaacga
     4381 aaccagtaat gtgatctggc tggcaacaaa ctgacttttg acggttgaag agattagcag
     4441 gccgatacct agagccacca gcagatacag catggatgcg ccggtgagca ccgtcaccga
     4501 gccgcgaaat ggaacggcaa acagaaactt ggcggcgagc aggcagagca ccaggccgat
     4561 catgccgagg atgaagtaag gaatggtttt gccgagcagg atttcatcgg tccggaccgg
     4621 agtcacgaac aacggttcca acgtgccgcg ctcccattca cgcgccatca caagagtggt
     4681 gagcaaagcg ccgatcaagg tcatgatcaa aacgatcaat ccgggcacga ggaaatagcg
     4741 gctctcgttg gcttcgttga accacatgcg gctttgcacc gtcgccggac tggcactccc
     4801 ttcgatgcct tgggcggctc gccgcgcact ccaccgtcca atcgccccct gagcatagtt
     4861 ttgaatgatg cgcgcccggt ttgcatcaac cccgtgcacg agtatctgca cttcggcgcc
     4921 accggcagtc agagtgcgag cgaagtcagg ccgaatgcgg atgatgccgt ccaccttgcg
     4981 agccaacatg agctcttcgg cattggccat tgacgtcatc aataccggct cgaaataggg
     5041 cgacagttgg aagccggcgg ctaattccgt cgtggccggc gatggatctt ccaggacaac
     5101 cgcgacggga actctggtca cgtcgagaga tagtccgtaa ccaaaaagca aaatgagcag
     5161 aaccggcatg acaatgccga tggcaatgct gcttggatcg cgcagcattt gtttcgcctc
     5221 tttgcgcacc aaagcgccga tgcgccgcga gttaaaacct gtcgtcatgc ggcaccacgg
     5281 gcgctacccg tctgaaccgg ttttgtccgc gcctcttcga caaccgctat gaaggcatcc
     5341 tccatagtcg actcgccttt ccgggctccg ctcgcctggc tgcggatggc cgcgggcgta
     5401 ccttgcgcga ggattttgcc accatccata atcgcgatgc gatcgcagta ttcagcttct
     5461 tccatgaaat gggtggtgat caccaccgtc actccttcct gggagagcgc ggtaatgcgc
     5521 cgccagaaat cgcgacgggc tagcggatcg gcgccgctgg tcggctcgtc aagaaagagc
     5581 gtttccggct catgcaggag cgccgccgcc attgccagac gctgcttaaa accgccgggc
     5641 agattgccac tgggcaggtt ggccaaggtg gatagctcga attgttgtag agcccaatcg
     5701 atgcggcaat ctttctgcat gccgcgcaag ttgtaagccc tggcgaaaaa ctccaaattc
     5761 tcgctgaccg agagctggcc gtaaagtgag aatttttggg cgacatagcc gatatgctgg
     5821 cgcgccgagg cgcgtgcgcg gcggagatcc actccggcca ctcgcaacgt tccctcactt
     5881 gcgggcaata agccgcaaag catgcgaaaa gtcgtagtct tgcccgcccc gttgggaccg
     5941 agcagtccga agatctcgcc gcgctgagcc tcaaacgtta catgatcgac ggcgataaac
     6001 tttccgaagc ggcgaaccag attttgaacc tgaacgacgg ccgcctcggg aggtcgggag
     6061 actggtgaat taggagataa ggagacgggg agacttagag aaagagaagc ggaggacaca
     6121 ggctgtttgt ttgcttgtct cgttgtctcc aggtccatct tttgtccgtc tttctctcct
     6181 tgtctccgtg tctccttctt tgatttgagc agcaccataa atccgtcttc gaagcgtggc
     6241 tccgctgaaa tagctatggc gtcgaagtta tctggatctc cttgtctctc ggtctccttg
     6301 tctcccgttt cgccactccg cacggtctcc ttcagcttcc cgctttcatc cttaagcttt
     6361 tccgtaacga tccggactcg gccgccctga gggacggcgt cgagcacttc agacttgtct
     6421 aagaggcgag cttgatattc tctggccttt tgaccggcag gtggttcggc aagaaatacc
     6481 cggccttggg ctaatgagct gatggcttcc ggatgacctt gcgccaaaat gttgccttgg
     6541 tgcatcactg ccacatggcc gcagagttcg gcctcatcga gataggaagt gctcactaac
     6601 accgtcaaat tttgctgact caccagctgc agaatgatat tccaaagttc ccgacgcgaa
     6661 agcggatcca caccgacggt cggctcgtca aggagcagta actcgggcgc acgtaccaat
     6721 gtgcatgcca gtcccaattt ctgcttcatg ccgcctgaca gccgcgcggc taatcgctgg
     6781 gtgaaaggcc cgagcgcggt catctccatt aagctggcgt agcgctggcg ccgttctttc
     6841 atggagacgc cgtgtaagtc ggcgtagaga tcgagatttt cctgaacgct cagatcttcg
     6901 tacaagccga atcgttgcgg catatagccg atccgatctt gcacgcgttg cggctcggca
     6961 gcgacatcga tgccgagaat tttcagatcg ccgccgtcgg gaagcatcaa tccggcggcg
     7021 agccggatca gcgtggtttt tcctgctcca tcgggtccta ccaacgccgt cagcgtgcca
     7081 tgagtcacat cgatcgaaac accgttcaat gcatcgactt tttcaccact gtcgcggcgg
     7141 aatgtcttgc ggagatcccg gccgaccaga gtgatcgggt tatcgctatc gttcatcgcg
     7201 gagctcgggg tctctattgg ttacctgtaa gtaaacgctt gccggcattc ccaatcgcag
     7261 ttcatcggcg ggatctttga cgaatacgcg gacctcataa accaggctgg tgcgcagttc
     7321 ctcggtctgc acggtcttgg gcgtgaattc ggcgaccggt gaaatgaatc caatccaacc
     7381 gtcgaaacgg cgaccggcaa agctgtcgac ttcaatagtc gcggccatgc cggatttgat
     7441 ttttccaaga tccggctcgg agatgaagga gcggacccac tttggatcgg taatcgctag
     7501 tgaaaacacc ggccgttgcg gcgaggccat ttcccccggc tccatcagtc gcgtgcgaat
     7561 caccgcatcc accggcgcga tcaattgagc atcttccaat tgctggcgca gaacgcgtgc
     7621 ttgtgcctcg aaggctctca gccgtgcttc attctcgctt atttcctccc ggcggggtcc
     7681 ggctatcgtc agatccaacg tcttttggac caccgccagt ttcgcttcag cgacttgcac
     7741 cgcggttctt gcattgtcga cgtcctgctg gcgcaccgcg cggccggcag aaatttcagc
     7801 tgcgcttctc cagcgctcat actgttgccg cgcgttcgtc agatcagcct gggctgcttg
     7861 caggttcgcc cgggcttgag cgatctcttc gggacggctg ccgctgcgca agcgcttcac
     7921 aacctggagt tgcgcggcgg cttgcgcctc agcctgggcc agctggggtt ccaatcttgc
     7981 cgtatcgagc cgcgccagca actgcccctt cctgacacga tcgccttcct gaaccaaaac
     8041 tgttgcgata cgctcactgt tgttgaaaga aagttgcacc tggcgcaggt cgatatcgcc
     8101 ataaagcgcc agaggcgtcg gatcaccctc gcgttggatc agccgccatg ctacgcccgc
     8161 ggcgatgagg cttagcagta tcaatcccgc aatcaaaagg cgccgtttca taccatagcg
     8221 ccgctcacgt tgtccgcgtc gctgatcatc gttgacgccc cttttcggtc aacacccctt
     8281 cgaaaaccac atttataatg gtcgaatagc ccctcttcgg ggttagatcg agttcggcca
     8341 ttcttatcgg attcataatc gcctcggtcg cgcccagaag aatttcaatg atcagccggg
     8401 ttgaaatatc cttgcggaca attccagctt tgcggcatcc ctccagtaag gaaccaaaat
     8461 atctttcaat tatttggcgc cgtcgagttt gcaccagctc gaacatctgc ggagcttcac
     8521 ggcggatgtc gcggacaaag ggcggccgaa tttcttcggt atgacgctgc aggcacgcca
     8581 gcagttgacg cagtgaatcg agcatgtcgt tggaaagagc ggtggcgatg agctgcagat
     8641 cggcctcgac cctgcgaaat ttgtccagca gcacggcctg taacaaatcg gtcttggttg
     8701 gaaaaaaggc gtacaaagtt ttcttgctca ttcccaattc gccggccaaa tcatccatgg
     8761 tgacgctgcg aaaaccgtgg gcaaaaaaat gttgccgcgc gacggcaatg attcgcgcag
     8821 ccgcatcatg gcgcggttga atgagactat cctttcggct tcgagacgat ggcatgcccg
     8881 taggtaccaa aaaaactata agagtataaa aagtttccgc gcgggcggca agagccacac
     8941 gcgaagcctg cagcagagcg gcgacggccc ctcgaccaac gggacctatg ttttgcgatc
     9001 agtgcccggc aactttgtcg ttgatcgatt atcgctctgc gctaagctcc ggggtccggg
     9061 ctcctcgctg cgaacgaaag aacgaagtcc ggtccaacac cttttcttga aattcctcaa
     9121 ggtacagata gatcacgggt gtgatgaaca aggtgatgaa ttgagaaacg actaggccgc
     9181 cgacaatcac caggcccagc gatcgtcgag acgcgccgtc agcgccaatt cccaacgcga
     9241 tcggcagcgc gccgaacacc gctgcgagag ttgtcatcag aatgggacgg aaacggtcca
     9301 ggctggcatc gtgaatcgct tggtcggccg gttcgccggc cacaactcga tggatggcga
     9361 aatcaacgat caagatgccg ttctttttga cgatccccat caacatgaac ataccgacaa
     9421 aagcgtagag cgatgcttgt tcgccaaaaa gccaaagggt taatagacct ccgaccaacg
     9481 cggtgggcag ggtcgataag acggtcagcg gatgcaagta gctctcatat aaaattgcca
     9541 gaatcacgta catgacgaaa acagcaagag ccatcaacac ggttagatgt cgcacggtat
     9601 cctgaaaagt ttgcgcttca ccttgcaggc tggcgcggac cgtcggcggc acgatttctg
     9661 ccgcggcctt gttgatgaaa tctgtcgcct ccccaacggc tactccgggt ttgagattga
     9721 agaacagagt tacactggtg aactgattga gatgattcac cgcctgcggg cctagcgatt
     9781 ccttccatgt taccagagtg ttcagcggca caagattttt gccgttatcg gatttgatat
     9841 gcagtaacga cagatcctga gcttccgagc gagctgcatc ttccgcttcc aaaatgactt
     9901 ggtactgatc ttccggtttt ttgattaaat acagatagtt ctgcgcgtag gcactgcgca
     9961 gcaactgaag aatgcgcgct tccgaaactc catgcaaccg cgcctgttcg cgctttattt
    10021 caatgtcgag gctgggcgta ttgttgaaat aatccgaaga taaactggca aatcccggaa
    10081 aaggccgcaa acgttccatc agcttgccgg cgacctcgta aacctgcttg ggattaaccc
    10141 ccgaaagcga gaatgcgtat tgtccctgat tttgattggt ggcgccggtg ctgatttcga
    10201 gaaccggacg tggccgcata aacggcatca ctcccgggac catcgcaagt ttgcccatca
    10261 agccgccagc gacctgttca atcggcgcgc gattgccggg ttcttcgaga aacgccagta
    10321 gcaaaccctg attcgaggca agaaactgac cattacccgt catggtaaaa gtggctctga
    10381 cgccggggtc cgcgcggaga atctcgtcgg cctgattctg aagctcgtgc atctgcccgg
    10441 gagaggcccc ttcgcggccg atcatcaacc caaaaataaa gctgctgtct ccgggcggaa
    10501 gaaaagcttt aggaacgagc gtgaaaagcc agacggttcc tgccaaacac acaacccaaa
    10561 tgagcgctga cacccagcga aaacgcagaa accaccagag ggcccggcta tagcgctcga
    10621 gaaaactttt ctcccaggcg ctcatcttcc tttccatcca cgtttgcttg gaacccttgc
    10681 cgcggtcgct taacatccga gcgcacatca gcggggtcaa agtcagcgac accaagccgg
    10741 aggcgatgat ggcgacgaca atagtcaccg cgaattcgcg aaagatcctt ccaaccaatc
    10801 ccggcatgaa gaccagcggg atgaaaaccg cagcgagcga aatggtcatc gaaatgattg
    10861 tgaagctgat ctcccgagcg ctggcgatgg ttgcttcaag agatttttgc ccgtgttcca
    10921 tccggcgcac cgtattttca agaaaaacga tggcatcatc gaccaaaaaa ccaatcgcca
    10981 gggtgagggc catgagcgat agattatcaa gactgaattc cagtaggtcc atgatcacga
    11041 acgtgattaa taaggataac ggcaaggcca ctgcgggtat tagggtgtcg gtggcgcgcc
    11101 cgaggaaaac aaatataacg atcaccacca gcgcaaacgc gatcgccaag gtcaactgaa
    11161 cgtcgataat ggagtgaatg atcgtctggg agcgatcata gatcggagtc accctcaccg
    11221 atccgggcaa ttcggtgctg atcagcggca aaagctggcg aacgctttga gcgacttcga
    11281 cggcattggc tccggcctta cggttaactg cgacgattac cgtggctgcg ggcggatcgt
    11341 agccgcgcac ccaaaaacgc atgttgatgc gctcgtcttg aactgaattt tgtacctggg
    11401 cgacatcgcg caaataaacc ggcgagccgt tcttgctgct gatgattaaa tcgctgtact
    11461 ccttggcatc atccaactga ccctgcgggc gaagcagtaa cgttccgccc acgccgtcaa
    11521 attgccccgc gccaatagtg cttgtaccgt tgcgaatcgc ttcggcgaga tcctcgacgg
    11581 aaatgccgcg cgcccacatg gcggaaggat cggctttgat gcgcactgcg gatttggcgc
    11641 caaaaacatt gacccggctg acccccggca gaatgctgat gcgctggcca acctgagtgc
    11701 tggcataatc gtagagctgg cccaccgtga cggaatcgct ggtgagcgct atgtacataa
    11761 tcggttgatc gttgggatta gtcttggagt acgtcggcgg cgaaggcaga tccaccggca
    11821 gattgccggt ggcctgagat attgccgttt gaacatcagt ggccgctaca tcaacgcttt
    11881 tgtccaacgc aaattgcaac gtcaagctgg tatgtccctg tgtgcttttg gagatcacca
    11941 gctccagccc gttgatctgc ataaactggc gctcgagagg agtagcaatg ctgtttgcca
    12001 cggtatcggg gctagcacct ggataattag cttgaacttg aattacggga taatccacgg
    12061 ccggcaaatc attgaccgga aggtgcaggt aactgacgat tccgaagaga acgaccgaca
    12121 cggtcaagac cattgtcatg actggccggc ggatgaataa ttctgagaag ctcatggttt
    12181 gctgccggat cgcgaggctg ctgcgggatt tttctcggac ccgggagcgg cgatctgaac
    12241 cttgccgccg ggggtaacac cgagctggcc attgacgaca acgcgctcac cttccttcag
    12301 gccttgcgca atcaccacta gatcgccctg gcgctgaccg acagtcaccg aacgcagctc
    12361 ggcgctttcg tcttgtttga caacgtaaac aaaggatccc ttggccgata gctgcggcgc
    12421 gtcggccgga accaaaaccg ccgaatgtcg ggtgtccaga atcaggcgga ttctggcaaa
    12481 ccgccccggc caaaaaagcc ggtctttgtt ttcgacagtg gcgcgcaact tgacagagcc
    12541 ggttccttct tgcaccgaat tatcgaggaa ggtcagcttt ccttcgcgcg ctctagtgct
    12601 ttcatcgtca agccgaacct ccgcgcgcaa actacggcgc gccatattac gttgcacttc
    12661 ggtaagatcg ctctcgggaa ttgtaaagtc ggcatagatc ggatccagtc tctggataac
    12721 cagcagcggg ctattgccgg gcgtcaccac attgccgaga tccaccagac gctgtccagc
    12781 acggccctct atcggtgatc gtatcgtgca gtattcaagg ttcaagcgag ccgtttccac
    12841 cgctgcccga ttttgccgta actgggcctc ggctacttcc acggcatttt tcttggagtc
    12901 gaaatcttgc cgcgaaaccg cgcgcggatc gctgacgctt tcaacccggg cgaagttggt
    12961 ctttgcaagg ttcaaggccg cctgcgcttg ggctacattg gcttcggctt ggttgagctg
    13021 tgcctgatac ggacgcggat cgatggtaaa cagcacatcg ccgatcttga cattggcgcc
    13081 atcggcgaaa tggatcttag taatgcgtcc cgagacctgc ggctgaatcg acaccacctc
    13141 gcgggcgacg atcttaccga cagcatcgat ataaatcggc acgtcttgac ttaccgcggt
    13201 ggcgaccgaa accggcgcgg gcgggcgctc aagagccgcc ggcgctttcc tgtcacatcc
    13261 ggccaacaaa gtaacggaca ggaaacccaa aatctgaagc actccaagag ggttccgagc
    13321 gccctgcgtc agccccgatc gtctcatcgc tttaggccac gagacgttca ggagcagctt
    13381 ggggaacgcg ctgcttcttc ctcgttgcgg ctcggacgtg ttcctcccat cgctttaggc
    13441 cgcgacgtat ataccccggt tcgagtccca gcgacgagca cacggagtta aatgaaaaaa
    13501 cgtgttcgac accgtccgtg aaaagccatt cgcgggcttc gtccaacagg gctcgcttct
    13561 tgccgctctc agcaaagata ttgtcctgaa aagtccgcac tccgtcttcc aaaacggcca
    13621 gcaacaaagc tttttccggc tccagaggaa tccttcgccg gtagttcgca aagtactcgt
    13681 ccgacatcaa agtgtccggc tgaaacaacg ggctcgaccg gtcctctgcg ccaaaacttt
    13741 tctctcttga ttcttcctct atagtagtct tcattggttc catttaatct cacattctaa
    13801 ggtgagcttc tactcaccca tgtcaattta atcacccaat aaggataatg ctttagaatt
    13861 gtagtgacgt ttgattgatt cttctcccaa acctacgccc cgaagcgcaa accaggtcgc
    13921 ctgctcgacc agggcatcct tcgacacgtt ataatcgatt gccggaactt tcgggtgcag
    13981 gtgcagcatc aacgagaacg caatgtggtg cacaaaccag acccgcaaat cgcggcggat
    14041 cggaatttcg ccaagatctc cggtctggac cgcctcgttc acgcaagctt cgaatttggc
    14101 gacccacgct ctggcgaatt tcttgtgcgt cagccgcacc agggctccgt cctcgagtag
    14161 actgcgcacg agcagacagt tcagagccgc tttgtgcgga tcgcccggac gctgtagcac
    14221 gtaatgcgag atggtgaaat agaccatcaa caccaaggtt gaagtcgacg gttcaagcgc
    14281 cagaatccgc ttgaactgcg caaaggtcgg ccccttggcg cacgcatcca acatcgcggc
    14341 atacagagat tctttgcttg gaaaatgttt atagagaagc gcttccgaaa caccggcggc
    14401 cttagctagc tcccgggtgg tcgtgccgtc aaaacccttc tccgcaaaga catcccgcac
    14461 agcatcgaca atcgctcgct tcctctgatc cgctgataac cgtgcgacat tccccatact
    14521 cgttagttag catttactca ccgaatttgt caagtttttt atctaccgaa cttaaattag
    14581 gtgtagacca ccgtgaccat agcgggccga gacctctctg cgcggcgatg tgccgcaggg
    14641 cgctcggcgc gtttcctacg gtaagcgctt aaagagattc taaagcaatc aagcaacaga
    14701 accgccgtgg gttgacaggt cctaagaacc atattagagc tgcgatagat tgctgccggt
    14761 tttgcccgca atcatcgaat gattctattg agtccattat ccaactcgct ctagatagaa
    14821 agttcatccc caaagaactt gcaagggcga ggctattaac taagcacgag cagaagtgac
    14881 ggacgaagga gaggcataga tggctttacg catcaacgat tgcgaatact atgcaatcga
    14941 cgcccattcc catttgggca gtcgccagac tccgttgggg cacggagtgg ccagttttct
    15001 tggcgatgac ttggtccgca atctcgacga agcgggactc gattgtgccg tggcgtttcc
    15061 tcttggagct tcctacacgg actattcgga cgcaaaccaa attatggcgg aggaaatggc
    15121 caagcatccc aagcgtataa ttagtttctg ccgcatcaat cctaattttg gcccacaggc
    15181 ttcggcgaaa tcgctggaac attgcctggg taaactcaaa ttgaaaggaa tcaaactcca
    15241 tccggaaatc gagttcttcg atcccaatga agcggagtta atggagccga tttatgctgc
    15301 ggctcggcgc tatcatgtgc cgattatttt tcacaccgga atgtccagca aggcatcacc
    15361 gggggtcatc gcagagcttg cggctcagta tagggatgtc ccggtcattc tcggccatat
    15421 gggtgtttcg gagtatgtaa agctggcggt ggcggtggct cggcagaacg aaaatatttt
    15481 tctggaaacc tcagtcgtcg gctggatgcc gctgctgctg gaggcctttc gcggcgttgg
    15541 cacaagtaag atcctcttcg gttccgatca tccttacaat ccattgtcca tggaagttga
    15601 aaaaatttcg agacacgtaa cccaagccgc caaactaaat caggccgact tgaaaaaggt
    15661 tttctcggac aactttttat cgctattgaa atgggaacag agagtctgat cgatttgttg
    15721 cacgtttttc cgccttcgaa caccgcatca aggagcttgg aaccatgcga tcttttagtc
    15781 ggattgtttt agtcgtgctc attgcagtag ccgtcctaac aggccaaaca gatgaatccc
    15841 tcgcgcagga acgaatccgg atcgcctggg ctggagtgag tccggcaaat agtccaattt
    15901 gggtggtcca agaaagaggg ttgcttaaga aacatgggct aaatggcgaa gtcattagta
    15961 tttcggcaag cccgattgcg ctccaggctt tgcttgcggg ggaagtagat gtgatcgtaa
    16021 cttccgtcac cacgcttgcc ctttctcgcc ttgcgggtgc cgatatcgtc atgattttag
    16081 gaatggtacc gactttccta gatcatatca tctccgatcc gagcgttacg accctcgagc
    16141 agataaaggg taagaccggg ggagtaaacc gacttggcag cacttcggat ttggggcttc
    16201 ggctggccct gcggcgcaaa ggcatcgatc cggaaaagga cgttaagatt atcaccgccg
    16261 gcgataaccc cgcgaggctc gcggcgattt ctcgcggaat cattcagttc agtattatgc
    16321 cagaaccgtt tgtccgggaa gctgagaagt taggctttaa agatctgttc gatatccaat
    16381 cattgaaaat cccgttttgg tggaacgctg tccttgctaa ggagtcggcc gtgaaaacaa
    16441 accgcgccgt gctgacaaag ttttcccgcg ccatgatgga agcaattcac tacatcaaga
    16501 ccgacaaaga aggaaccaag gcgatctaca gtaaaaatct caagcttacg gatccagaag
    16561 gtctggaaag agcgtaccaa gcttatgtct ccgtgtttcc ggaaaatttg ctgcccagtc
    16621 cggatggcgt caaaaccatg ctggatgatc ttgcgccacg aaatccaaag gcagcggcgg
    16681 ctgatcctaa gacgttcgtc gacatgagtc tagtccaaga aatcgaagct agcggcttca
    16741 tcaaacaact gtacaagcga taattggtta atcttaacgc gggcacaggg aatcaccggc
    16801 aggagagcgc gcttctgcac atttaataaa atagttacaa gacaacagat ttctgacctt
    16861 agaaaagaga tgacattgcg aaacgcggag gttaaatgag gctagcgaga atctcggtcc
    16921 gaggctgcgc atcggcaggt cgattggtcg ctttgctgtc cgtcttttac attttgaccg
    16981 gttcgttgtc agctcgagcg gctgagatgc ccctggtacg tgccaactat gcggccatca
    17041 gcggcgcgtt tgcgccgctg tggatcgcaa ccgagaaagg cttattcacg aaatatggct
    17101 tgaatgtcga tctaaggtac attgcccctg ctactgccac gcaggcactc ctagccaaga
    17161 acctcgatat tatcaacccc ggcggtgaga tcgtcgaagc cggattaaac ggcgagccgg
    17221 tggtgtttat cgcaggaatt ctcaatcgag cggtcatgtc gatctatgca aagccggaga
    17281 tcaattccat gtccgatctc aagggcaagg tccttggcgt taccgtgccc ggagcgacca
    17341 ctgattttgc cgcgcgtgtt ttgctccagc aatcgggatt ggcgccggga aaagatgtca
    17401 aacttcttta tctcagagga atgccggaaa tcctggcggg gataaaccag ggaaatgcgg
    17461 tggccggaat ctttacagca ccgacaactt tgaaagcacg cgccgcagga ttgaaagaat
    17521 tggtgaatgt aacggaacag aatattccaa tgatccacgc cgcgctagcc accacgagag
    17581 attatctcaa gattaaccat gatccgacgc agcagttttt gcgcgcatat ctcgaaggca
    17641 ttaaaatcgc tctcactgac gcggaattca ccaaacaaat gattggcaag tacaccaaaa
    17701 cgacggatgc gaaagatctg gagagctctt atcaagcatt tttacccgct tgggaacggc
    17761 ttcccatcgt cccggctgcg gctgtacaaa cgatgctcaa tttcgcatcg catccgggtg
    17821 ccaaagtggc caaacccgaa agtctcatcg acaactccat actcatggaa ctcggtaagt
    17881 caggattcgt cgataagctt tataaatgat cttggtaaca agccgattca tttatatatt
    17941 tatatactgc aagaccggac cccgcccgct gttgcccgtt cgtttagcga ataagttcct
    18001 cgatcttttt tttaagttgc acgatggtaa acggcttatt gatgtagccg ttgcaacctt
    18061 gttctatgca ggtattgaga tccgattgat gaaacaacgc tgttgctgcc aaaatcggga
    18121 tgtccttggt ttcaggattg gcccgtagac ttcggacggc ttcccaacca tccataatcg
    18181 gcatgatcat atcgagcaag atcaatcgcg gcttctctcg catagctttc tctaagacgt
    18241 ctttaccgtt ttcagcgaga atcggaagaa agcccaacga ttctatttgc ctggccagca
    18301 actcacgcat atcctgatag tcatccgcga tcaaaacctt tttcattgaa gtccccgtca
    18361 tgcttcaacc aaggagctgg ttaatctggc gcttggcgga aaccgcggat ccggtgatag
    18421 ttccaagtga acccattcgc attggctttc acgatgatgc tgaatacatc tagggcagac
    18481 cgttgccgaa tgggaatcta tatcttttcg ttcaccgtat ccaaattgag gccagttctc
    18541 gcacttcctg cagaaatgcc atacattctc gtgcggcagt tttcgataaa aagttctgat
    18601 atcgaacatg cgagctcctg atcagctgag gtgattacga accgcaggag aaaggggtgg
    18661 tgatgcaatc gatgactttg tcccaactcc ctgccaattc atcgcctatg ttggtgctct
    18721 tgatgccccc ccagaaaacc agagcgacca gaaatatgat cagggtgtat tccatcaatc
    18781 cctgaccccg ctcattacgc aagttcttta gtggtctcat gactctcccc tttcgttgct
    18841 ttatgcgatg ctgtggtatc acaacccggc ttagcgcaac tctaatgcca cag
//
LOCUS       DASUVS010000083         3357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_28984, whole genome shotgun sequence.
ACCESSION   DASUVS010000083 DASUVS010000000
VERSION     DASUVS010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28984"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VF182_04850"
     CDS             <1..796
                     /locus_tag="VF182_04850"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX6436436.1"
                     /translation="FSLPECHIPLRRSALYRRVILTSIRRAHLVSVPSQFTRQAILEF
                     LPDIPPERIHVIPAGVGEEFHPQAANDIQSAKSRLQLNWPYILYVGTIEPRKNLQRLV
                     ESYQRLIAKGGVREHLVLAGRLGWDYKALLEQINAPALRDRVHLPGYITQGDLPLLYA
                     GARLFVYPSLQEGFGFPPLEAMACGVPTVASLSSSLAENLRDAAELVPADDTEALTVA
                     MGRLLHDSQLHAKRRTQGLARAAQFRWQETARQTLNCYQALAANRS"
     gene            1256..2083
                     /locus_tag="VF182_04855"
     CDS             1256..2083
                     /locus_tag="VF182_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6436437.1"
                     /translation="MNPTPNILIEAPPRWEALDLCELWRYRELLYFLALRDVTVRYKQ
                     TVIGAGWAILQPFLTMVVFSIIFGQLLKVPSDGVAYPVFSYAALVPWNFFAGALTRSG
                     NSLIADANLISKVYFPRLILPLAAVLSLILDFAIAFVILVVMMLFYHIVPSVAVLTLP
                     LFLVLALITALACGVWLSAVNIKYRDVAYVIPFLTQFWLFVTPVAYPSTIIPEPWRVF
                     YGLNPMAGVVEGFRWALLGQENPAWDLILVSVLVVLALLISGLFYFRRMEHEFADVV"
     gene            2254..>3357
                     /locus_tag="VF182_04860"
     CDS             2254..>3357
                     /locus_tag="VF182_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011450026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6436438.1"
                     /translation="MRDIAIRVENLSKHYKIGVAKYRHDTLRDQIMVNLKSWFGRTCH
                     PSGANGQSSSVANHPSSFWALRDVSFEVKRGEVLGIIGRNGAGKSTLLKILSRVTRPT
                     HGRARINGRVGSLLEVGTGFHPELTGRENIFLNGAVLGMKNTEIQQKFDEIVEFAEIE
                     QFLDTPVKRYSSGMYMRLAFSIAAHLEPEILIVDEVLAVGDAAFQKKSLGKMGAVAKE
                     GRTVLFVSHNLAAIRSICERAILIEEGKIRLDGKPHDAITTYLLSGAGDMSEDEGQVL
                     WTDPNVEPPGCEEFWLRGIQLKGPSGMVQSVFEADKPIHVEFYYEIKRPLRDAKFYLH
                     VLTQEGENAFSGTDCNFREEIESPGFYRSTCIIP"
BASE COUNT          740 a          887 c          910 g          820 t
ORIGIN      
        1 tttttcactc ccggagtgcc atattccgct gcgccggagc gcactctacc gccgtgtcat
       61 attgacaagt atccggcggg cgcacctggt gagtgttccg tcacagttca cccggcaagc
      121 aatactagag ttcctgccgg atattcctcc cgaacgaatt catgttattc cggctggcgt
      181 tggggaggaa tttcatccgc aagcggcgaa cgatatacag agcgcaaaat cgcgcctaca
      241 gctgaattgg ccttacattc tgtacgtcgg cacgattgag ccgcgcaaga atttacaacg
      301 cctggtggaa agctaccagc ggctcatcgc caaaggtggc gtccgtgagc atctggtcct
      361 ggcaggccgg ctcggttggg actacaaagc gctcctcgag cagatcaacg cgcctgcttt
      421 gcgcgacagg gtgcatttgc cgggttacat cactcaaggt gatctgcctt tgctctatgc
      481 gggagcaagg ctatttgtct atccgtctct gcaagagggt tttgggtttc cgcctctcga
      541 ggccatggct tgtggagtgc cgacggtcgc atcgctttcg tcatcgctgg ccgagaatct
      601 ccgagacgcc gccgagctcg taccggccga tgacacagaa gcgctaacgg tcgcgatggg
      661 ccgcttatta cacgatagtc agctacatgc aaaacggcgg acccaggggc tcgctcgagc
      721 ggcccaattc cgatggcaag agacagcgcg gcaaacgctg aactgctacc aagctctcgc
      781 agccaacaga tcctgaccct tcacttggag gttgtcagac catttaggct acgggtttag
      841 tccaccgata ccttcgcaag cggacaccaa gcttcgacag aagattaagt tgaggatgtg
      901 tcaaattgcc gctcgaccat aatcttagcc taaacttgac gttaaccagt cttatgttgt
      961 tgagcgcgca agacggagcg cgcaacaaag cctatgcttc ctttcacgac cctagcacag
     1021 caccaaaaaa cgtggggtat gccgaagatt ctcgttgatt ttacgacgac ctagccaatt
     1081 gcccgtgacg cgcaaccgtc tcgtcgtacg ggtgatcgac atagatcgtc cgggagccgc
     1141 tttcaaaaat cattgttgca aacaaggtcc gataattttg ctaaagccta aaacaaccgc
     1201 cgcggaagag gaaactaatc acctcattca ctggccagac tcggcggaat ttcgcgtgaa
     1261 tcccacgccg aacattctga tcgaggcgcc accgcgttgg gaagcgctcg atctgtgcga
     1321 gttgtggaga tatcgagagc tgctttattt tctcgcgttg cgggacgtca cggtacgcta
     1381 caagcagacc gtgattggcg cagggtgggc gatcctacaa ccttttctca ctatggttgt
     1441 tttttcaatt atcttcggcc aattgcttaa agtgccatcc gacggcgtag cctatcccgt
     1501 cttctcctat gctgccctcg tgccatggaa ctttttcgcg ggcgcgctca cgcgctccgg
     1561 caatagtctc attgccgatg ctaacctgat ctccaaggtc tattttccgc ggttgatcct
     1621 gccgctggcg gccgtgttgt cactcatcct cgacttcgcc atcgcctttg tcattctggt
     1681 ggtgatgatg ctgttttatc acatcgttcc cagtgtcgcg gtgctcacgc tgcccctatt
     1741 tctagtgctt gctttaatca cggcactcgc ttgcggcgtc tggctttcgg cggtgaacat
     1801 caagtaccgc gacgttgcgt acgtcatacc gtttctgacg cagttttggc tctttgtgac
     1861 gccagtcgct tacccaagca caatcatccc cgaaccctgg cgtgtttttt acggcttgaa
     1921 tccgatggct ggagtggtcg agggtttccg ctgggctctc ctaggccagg aaaatccggc
     1981 ctgggatctt attctcgttt ccgtcttagt cgtcctggcc ctgctcatca gcggcttatt
     2041 ctacttccgc cgaatggagc atgagtttgc ggatgtggtg tagcgtacaa agacgcgaga
     2101 gcgttgaccg agaaccgtcg acggggatcg gcgatgtgtg gtttaccacg taggactctg
     2161 aatcgttaat ggtggcgagc cgggctcgac taaccaccct ttttgagtca ttcggagttc
     2221 ttatcaacga tagactccca tcaaagacga ataatgcgcg acattgctat tcgggttgag
     2281 aacctttcca agcactacaa gatcggtgtc gcaaagtatc gtcacgatac gctacgcgac
     2341 cagatcatgg ttaacctcaa gtcgtggttt ggccggacct gccatccgtc cggggccaac
     2401 ggtcaatcat cgtcggtcgc caatcatcct agtagcttct gggctcttcg cgatgtctcc
     2461 ttcgaggtta aacgcggcga agtacttggg attatcggcc gcaacggagc gggcaaaagc
     2521 acacttttaa agatcctctc gcgagtcacg agaccgaccc atggccgggc acggattaat
     2581 ggccgtgttg gctcgttgct tgaagtaggc accggttttc atccggagct gaccggccga
     2641 gagaacatct tcttaaacgg cgcagttctg ggcatgaaga atactgaaat ccagcaaaag
     2701 ttcgacgaaa ttgtagaatt tgccgaaatt gaacagttct tggacacgcc cgttaagcgc
     2761 tactcaagcg ggatgtatat gcggctggct ttttcaattg cggcgcacct tgaaccagaa
     2821 attctgatcg tagacgaggt gctggcggtt ggagatgctg cgtttcagaa gaagtctctc
     2881 gggaaaatgg gcgcagtggc gaaggaaggt cggacggtcc tgtttgtgag tcataatctg
     2941 gccgctattc ggtcgatctg tgagcgggcg atcctaatag aggaagggaa aatccggctc
     3001 gatggaaagc cgcacgacgc gattactact tatctgttat ctggggcggg tgatatgtct
     3061 gaagatgagg gacaagtcct ttggacggac cctaatgtcg aacctccggg atgtgaagaa
     3121 ttctggttgc gaggaatcca gctgaaaggt ccatctggga tggtgcaatc ggtatttgag
     3181 gccgataaac caatacacgt ggaattctac tatgaaataa agcgaccgct cagagatgcg
     3241 aagttttatt tgcacgttct cactcaagaa ggtgaaaacg cattctcagg aacggattgt
     3301 aatttccgcg aggaaataga atccccgggg ttttacagga gtacgtgcat cattcct
//
LOCUS       DASUVS010000084         4612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1776813, whole genome shotgun sequence.
ACCESSION   DASUVS010000084 DASUVS010000000
VERSION     DASUVS010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776813"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VF182_04865"
     CDS             <1..1017
                     /locus_tag="VF182_04865"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6436439.1"
                     /translation="MILLGFLFFGMNAQAQTPFYQGKTITVIIGTPPGNLYDLWGRLI
                     VEHMGKHIPGNPAFIVQNMPGAGHVIAVNHLYKVVKPDGLTIIGSVIPSLYLNQLIGR
                     PEIQFDWSKFTWIGSPARGASQMYMRSDTPYKTIEDVRTAKEPPKCGATGVTGPDSYY
                     PKLLQETVGAKFTIVSGYPGGTDIDLGVERGEIHCRAFTIEAFFGREPYHSWRKRGFV
                     HHLFQSGKTRDSRLPNTPTIFELMDQYKTNESGRRLATVILGADGMGRPMFGPPGMPA
                     DRLAILRDAFTKTMADEQFRAEVKKRNYEFDPIAGEELQDLGKEVTSQPPETIERLRK
                     LLSN"
     gene            1405..1941
                     /locus_tag="VF182_04870"
     CDS             1405..1941
                     /locus_tag="VF182_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX6436440.1"
                     /translation="MPNEKNSVGEVQEAIRKGAITREYRADTKRVIQALNGLRSTEIM
                     SYLQYMQHQYMAVSLLSPGLKTEFEAHATQELDHANRLAERIQQLGGVPVYDPKEIST
                     KAANVGVQPEQGTTLTEMVVEDLLLERQQIEVYSALIREIGDEDLVTRHVLLGILADT
                     EKHASELSDYLKRTADTH"
     gene            2005..2229
                     /locus_tag="VF182_04875"
     CDS             2005..2229
                     /locus_tag="VF182_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HEX6436441.1"
                     /translation="MAKELKSGDRVQWNTPQGKTTGTVKKKLVSARKIKGHVAKASKE
                     NPEYLVQSEKSGKQAVHKAGELHKARNRPK"
     gene            2277..3662
                     /locus_tag="VF182_04880"
     CDS             2277..3662
                     /locus_tag="VF182_04880"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX6436442.1"
                     /translation="MVRSEIPAERIQLLNARAITPGAYVAYWMQQSQRAEYNHALEYA
                     IQQANELKLPVHVFFGLTDDYPEANLRHYTFMLEGLRETQAALTARGIAMAVQKGDPV
                     SVAVTAARQAALLVCDRGYLRHQKRWRRQVSENSPCRVVQIETDVVAPVEAISGKREV
                     AARTLRPKIHQQLGKYLVEMIETKVVRRSAPAEPTGLDLSDLEETLRQLKIDRRVTSV
                     SRFFTGGLSQAKKRFATFLRRKLAHYKDNRNQPQTDYVSHMSPYLHFGQISPLFLALG
                     IWRRRAADRANAETYLEELVVRRELSMNFVEFTDNYDSITCLPEWAKQTLNDHRRDAR
                     EHLYSPSEMERAKTHDRYWNAAWLEAKRTGYMHSYMRMYWAKQILRWSRTPEEGFDTA
                     LALMNKYFLDGRDANSYAGVAWVFGNHDRPFYDREVFGTVRSMTASGLKRKADMEGYI
                     AKVESFRESDE"
     gene            complement(3825..>4612)
                     /locus_tag="VF182_04885"
     CDS             complement(3825..>4612)
                     /locus_tag="VF182_04885"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436443.1"
                     /translation="PRIVFIDARVAIAALVAGEVDAVVISAPSVIPAVLSGANIAFIA
                     GLHNKMIFSFHAQPDIQSSAQLRGKIVGTNRPGTPADYGNRVALTKMGLKPTDVQLMP
                     LGSSGVMWSALQSRQVAGVTLAPPYSFRADAMKFSRLVDTYDKPYQNTGVVVRKDRIH
                     SSADTWLRLLRALQQANLRWYEDPKLAMFVLSKYTQQSDPDVLQKTYDFESNPPGFTK
                     DLKVSDSGLQGILDFLASTVRPEAGKASPKEFYDTTILDRLEK"
BASE COUNT         1157 a         1236 c         1220 g          999 t
ORIGIN      
        1 atgattcttc tgggtttttt gtttttcggc atgaacgcac aggcacagac gcctttctat
       61 caagggaaaa ccatcaccgt gatcatcggc acgccgccgg gaaatcttta tgatctctgg
      121 gggcgtttga ttgtcgaaca catgggcaaa catattccgg gcaatcccgc tttcatcgtg
      181 cagaacatgc ccggcgcggg gcatgtaatc gcggtcaacc atctttataa ggtcgtaaaa
      241 ccggatggct tgacgatcat cggttcggtg attccatcac tctatctgaa ccagttgatc
      301 ggcagaccgg agattcaatt cgattggtcc aagttcactt ggatcggctc cccggcgcgg
      361 ggcgcaagcc agatgtacat gcgcagcgac acaccgtaca aaaccatcga ggatgtccgc
      421 accgccaaag agccacctaa atgcggcgcg actggcgtca ccgggcccga ctcctactac
      481 ccgaagctgt tgcaagagac cgtgggcgcc aagtttacca tcgtgtcggg ttatcccggt
      541 ggcaccgata ttgacctcgg cgtcgagcgc ggtgaaattc actgccgcgc ttttaccatc
      601 gaggcattct ttggccgcga gccgtaccat agctggcgca aaagaggttt cgtccaccat
      661 ctgtttcaga gcggcaaaac tcgcgactcc cgcttgccga atacgccgac aatcttcgag
      721 ctcatggatc aatacaaaac caatgaatcg ggacggcgac ttgccaccgt gatactcggc
      781 gccgatggca tgggacggcc gatgtttgga ccgcccggca tgccggctga tcgtttagcg
      841 attctgcgcg atgctttcac aaagaccatg gcagacgagc agttccgcgc ggaagtgaag
      901 aagcggaact atgaattcga tccgatagcg ggcgaggagt tgcaagacct ggggaaggaa
      961 gttaccagcc aaccgccgga aactatagag cgtttaagaa agcttttgtc gaactaatat
     1021 ttcagcggga gaattctttt tctcccggct cgctgaagct tgcttgtatt tgcgattgcg
     1081 gaacccctct atcaacatga actttcatcc aaaaacgcac cggcgcatcg cgaccgcagc
     1141 agtccaagga gatcgttact gctacttacc gagaaatatt ccggatcgat cgcttcttac
     1201 gttggattga gcttcttcaa ccccgcagca tctttcacgt cccgaattcg ggaatctctg
     1261 aattcatttg tcttagtttg ggaattacgt cgaaaagagt tctaatcttt ctcgctccaa
     1321 ggaaaagcgc ctcgttggta ttgatcttgc gccattttgc tcagcaccaa cgagacgtcg
     1381 ccctctaaac cgaaaggaga aatcatgccg aatgaaaaaa atagcgtcgg agaagttcaa
     1441 gaagcaatca gaaaaggcgc cataacgcgc gaatatagag ccgacacaaa gcgagtcatc
     1501 caggctttga acgggctacg atccacagag atcatgagct atctacagta catgcagcat
     1561 cagtatatgg cagtttcctt gctgtcgccc ggtctcaaga ccgagtttga agcgcacgca
     1621 acgcaagagc ttgatcacgc caatcggttg gctgaacgaa ttcagcaact cggcggcgta
     1681 cctgtttacg acccgaagga gatatcaacg aaagcggcaa acgtgggtgt ccaaccggag
     1741 caaggcacga cgttgactga aatggttgtg gaggatctct tgcttgaaag acagcagatt
     1801 gaagtctaca gcgccttaat tagagagatt ggtgatgaag atctggtaac ccggcacgtg
     1861 ctgctgggaa ttcttgccga cacggaaaaa cacgccagcg aattgtccga ttacctcaaa
     1921 agaactgcgg atacacattg atcgaacgga ggctgccgat ggattgtcga tctatcggcg
     1981 gcatctacaa aggcgcattt tcttatggct aaagaattaa agtccggcga tcgcgtgcag
     2041 tggaacactc cccagggaaa aaccaccggc acagtcaaaa agaagcttgt ttccgccaga
     2101 aaaatcaaag gacatgtcgc caaagcgtca aaagagaatc ctgaatacct agttcaaagc
     2161 gaaaaatccg gcaaacaagc ggtccataag gccggtgagc ttcacaaggc gcgcaaccgc
     2221 cctaagtgac ctgattccat tcgatgatcg aagcccgggc agtgaacgca accacaatgg
     2281 tgcgatccga aattcccgcc gagcgtattc agttgctcaa cgccagggcg attacccccg
     2341 gcgcttacgt agcttactgg atgcaacaat cacaacgtgc cgaatacaat cacgcgctgg
     2401 agtacgccat ccaacaagcc aacgaattga aactgcccgt ccatgtcttc ttcggcctca
     2461 ccgacgatta ccccgaggcc aatctgcgcc attacacttt tatgctggaa ggtttacggg
     2521 aaactcaagc tgcgcttacc gcacgcggga tcgcgatggc cgtacagaaa ggtgatccgg
     2581 taagcgttgc agtcacagcc gcacgccaag ccgcgctcct agtctgcgat cgcggctacc
     2641 tgcgccacca gaagagatgg cgccggcagg tcagcgaaaa ttcgccgtgc cgtgtagtgc
     2701 agattgagac cgacgtcgtc gcgccggtcg aggcgatttc cggcaagcgg gaggtggccg
     2761 cacgcacact ccgcccgaag atccaccaac agctgggcaa gtatcttgtt gagatgatcg
     2821 agacaaaggt tgtgcggcgc agcgcccccg ccgaaccaac tggcctcgat cttagcgatc
     2881 tggaagagac tctgagacag ctcaagatcg accggagggt aacatcggta agccgctttt
     2941 ttactggcgg cttatcgcaa gctaaaaagc gcttcgccac atttttacgg agaaagctcg
     3001 cgcactacaa agacaaccgc aatcagccgc aaaccgacta cgtatcgcac atgagcccgt
     3061 acttacactt cggtcagatc tcccctctat ttctggcgtt gggcatttgg cggcggcgcg
     3121 cagccgaccg cgccaacgcc gagacttact tggaggagct ggtcgtgagg cgcgagttat
     3181 cgatgaactt cgtcgagttc accgacaact atgattccat cacctgcttg ccggagtggg
     3241 caaaacaaac tctcaatgat caccgccgag atgcccggga gcatctttat tcgccatcgg
     3301 aaatggagcg ggctaagaca catgatcggt attggaatgc ggcatggctc gaagccaagc
     3361 gaacgggata catgcacagc tatatgcgca tgtactgggc gaaacagatt ttaagatggt
     3421 cgcgcacgcc ggaggaaggt ttcgataccg ccctggctct aatgaataaa tattttctag
     3481 acggccggga cgccaactcg tacgccggtg tcgcctgggt tttcggcaat catgaccggc
     3541 ctttttacga tcgtgaagtt tttggaacgg ttcgctccat gaccgcgtcc ggcctgaaaa
     3601 gaaaggcaga catggaaggt tatatcgcga aagttgaaag ctttagagaa agcgatgagt
     3661 aaagtatcgc cgtgcgtgta gatttataaa gcccccctta gttcgccccc acacttaatt
     3721 gagaatcctt acttacaaag ccggtagttt cgctttaacg accgtcgatc gaggccgcat
     3781 cagcgttgaa ttaagcgctt taactagtaa ttgttcgggc tgattcattt ctccagccga
     3841 tccagaatcg tagtatcgta gaattctttc ggagaagcct ttccggcttc agggcgaacg
     3901 gtggaggcta aaaagtcgag gatgccttga agaccgctgt cggatacttt taagtccttg
     3961 gtaaacccgg gtggattcga ttcgaaatcg tacgttttct gcagcacatc cggatcggat
     4021 tgctgggtgt atttactaag cacgaacatc gcgagcttag gatcttcata ccagcgtaga
     4081 tttgcctgct gcagagctct gagcaaacgc agccatgtat ccgcgctaga atgaattcga
     4141 tctttgcgaa ccacgacgcc cgtgttctgg taaggtttgt cgtaggtatc caccaggcgg
     4201 gaaaacttca tggcatccgc tctaaagctg taaggcggag cgagcgtcac gcccgcgacc
     4261 tggcgcgatt gcaacgccga ccacattact cccgaacttc ccaatggcat aagctgcacg
     4321 tccgtgggct ttaaacccat ctttgtcagc gcgactcgat tgccgtagtc ggcgggagtg
     4381 ccgggccgat ttgtcccgac aatctttccc cgaagctgcg ccgagctttg tatatccggc
     4441 tgcgcgtgaa aagagaagat catcttgttg tgcagtcccg cgataaaagc gatgttggcg
     4501 ccgctcagga cagcgggtat taccgatggc gcagagatca cgacggcatc gacctcgcct
     4561 gcaacaaggg cagctatagc gactcttgcg tcgatgaaga cgattctcgg gt
//
LOCUS       DASUVS010000085         4085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_29260, whole genome shotgun sequence.
ACCESSION   DASUVS010000085 DASUVS010000000
VERSION     DASUVS010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29260"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VF182_04890"
     CDS             <1..793
                     /locus_tag="VF182_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6436444.1"
                     /translation="RVGIAIGDLGQTPPYVEFTKRLSMLRESTLFLKEALSGNTAFFR
                     EYPTLVRYFRLNPEGKLKLAFAAPGPLPFYGGTLGPKSLAVAGEHMEGIVFPGQFLAF
                     FKTGRLQTMIQTAKDAAARSGKNKKLRFSALLNVSVSKDRKQARRFALPQVAHSVVSL
                     KVAQFTHEDFDKVGIDPDRVDRIQEAFTKGGGVTIEEAARLVDDRMIDAYYLAGTPEE
                     VTPPLINLVRELGQLGIEEIAFSKLGYDYREGLELIAKEVIPHVR"
     gene            1196..2347
                     /locus_tag="VF182_04895"
     CDS             1196..2347
                     /locus_tag="VF182_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436445.1"
                     /translation="MPIFLTNEQQAACITAAEAVAAMAEGCKQFSFGDAIRRPRIDNY
                     LPTSRPTEHFCFSSMEGGVRSGYYALRIKPDILSWPVLNGMRRRTSYCTKPGLYGGLV
                     FLFSTENAELLAIMNDGYIQHVRVAATAALGIQYLARRNATVIGMFGSGGMARHFAQA
                     AKAVMPVRRLEVFSPNWNNLKTYCSEMEQQLGYEVIPHKDPKTTAQNADILCLCTNSQ
                     DPVLQAEWMKPGIHLTNVRTTELSREVCAKIEIAGLLTRRTPPSVANFVDDDFEIRGD
                     SMAYVGGQPEEREKIPRGVPTTDRYPNAKYVDCCDWKTGKPYERGRGDEITILATNSM
                     GIHEGDAGASAGNQGIQFATVAGRIYENARKLDLGQKLPMEMFLQDIPT"
     gene            complement(2599..3708)
                     /locus_tag="VF182_04900"
     CDS             complement(2599..3708)
                     /locus_tag="VF182_04900"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436446.1"
                     /translation="MRSSGVDIVSLHAIRADQFNEPNPLMQQIAFKTLALQSLRAAVV
                     LMLIFSNPFCRADAQTNAVRISYSAPSGLSTPAWMAKDLALFEKHGVNGSLIYIGGGT
                     LPMSVLLAGDTDFTMVVGPPAVLARLNGADSVILASFVNRLTMSFVSLPSIRQPTELR
                     GKRVGITRFGAATDYAAKLALKSWGLTSKDVEIVQTGGIGEAFAALKSGLIQASVFSP
                     PQSTQAIRSGMTELLDLSRSEIQFVNNSLVSTMKYVQQRPGVTHNVMKALIEGIWAFK
                     ADRELGTKVLEKYTRVQDRRVLDETYDFYSRVLQDLPRTSEPGLSNILEALSETQPKA
                     RTATPKDFFDPKFMTQLEKDGFFKQLSARYKSAAR"
BASE COUNT         1004 a         1056 c         1058 g          967 t
ORIGIN      
        1 acgagtcggc atcgccatcg gagacctggg acagacacct ccgtatgtcg agtttaccaa
       61 aaggctctct atgctgcgcg aatccactct gtttttgaag gaagcgttgt ccggaaatac
      121 ggcattcttt cgtgaatatc cgacgctcgt gcgctatttc cggttgaatc ctgagggaaa
      181 actgaagctg gctttcgcag ccccgggacc gctgccgttc tatggaggaa ccctgggacc
      241 caaatctctc gcagtagcag gcgaacacat ggaagggatt gtctttccag gacagttcct
      301 cgcatttttc aagaccggcc gactgcagac gatgattcag acagccaaag acgcggcggc
      361 gcgttcgggg aagaacaaga agctacgttt ttctgccctt ttgaacgtca gcgtatcgaa
      421 ggaccggaaa caagctcgcc ggttcgcgtt gcctcaagtg gcgcattctg ttgtgagcct
      481 aaaagtggct cagtttaccc acgaggattt tgacaaagtc ggcatagatc ccgaccgggt
      541 ggatcgtatt caggaagcgt ttacgaaggg tggaggcgtt acaatcgaag aagcagcccg
      601 actagtcgac gaccgcatga ttgacgctta ctacctcgcg ggaacacccg aggaagttac
      661 acccccactc atcaatcttg tccgagaact gggccaactt gggattgaag aaattgcgtt
      721 ttccaaatta ggttacgact accgagaagg gttggagctg atcgccaagg aggttatccc
      781 ccatgttcga tagcgtctgc tgaccatccg cggcgactgt cgaactctca caaagggctg
      841 aaggtccgat tccaatgtcg tgacatcacc actctcaatc gtgagttgaa tcttaacaga
      901 gttctttcgg cttcaaaaaa gcgatcgtcc gaaatttctc cagtacgaac tgagccatat
      961 tgcggctgcg aacatctaac agtcggcgtc ccgtctcggc cttgcgaccg gacctactgc
     1021 gtcgatctac atcaacgaag tttaggttcg cgagatggaa cattcgcctt cccttgttct
     1081 taccgcgagg cgtaaacggc tggcttggca tttctcgcag tttattgaca ccaaagtgtc
     1141 gtatcaagta gattcgcggc gagcattcca acgatgcgac gaaacgagga ttaagatgcc
     1201 tatcttttta accaacgagc aacaagccgc atgcatcacc gcggcagagg cagtagcagc
     1261 gatggccgaa ggctgcaagc agttttcctt cggtgatgcg attcggagac caaggataga
     1321 caactactta cctacgtcgc gaccgactga gcacttctgt ttcagttcta tggaaggtgg
     1381 agttcgatca ggatactacg cgctacgcat caaaccggat attctgtcat ggcccgtctt
     1441 aaatggaatg cgccgccgaa cctcctattg cactaaacct ggcttatacg gcggattggt
     1501 atttcttttc agcacagaaa acgccgagct tcttgcgatt atgaacgacg gctacataca
     1561 gcacgtgagg gttgccgcca ctgcagcttt gggcatccaa tatttagcaa ggcgcaatgc
     1621 aacggtcatt ggtatgttcg gttcaggtgg gatggcgcgc cattttgcac aagccgctaa
     1681 ggccgttatg ccagtacgca ggctggaggt ttttagccct aattggaaca accttaaaac
     1741 ctattgtagt gagatggaac agcaacttgg gtacgaagtc ataccccata aggatcctaa
     1801 gacgactgcg caaaatgcgg atattttgtg tttatgtact aattctcagg atcctgttct
     1861 ccaagccgaa tggatgaaac ccggcatcca tttaaccaac gtcaggacga ctgaactatc
     1921 tcgcgaagtc tgcgctaaga ttgagatcgc cggtcttttg actcgaagaa caccgccgtc
     1981 tgtcgctaac tttgtcgacg acgatttcga gattcgtggc gactcaatgg cgtatgtggg
     2041 tggacagccg gaggaacgag agaaaatacc gcgcggtgta ccaacaaccg atcgataccc
     2101 caatgccaaa tatgtcgatt gctgcgactg gaaaactggt aagccgtacg aacgcggccg
     2161 tggggatgag attacaattt tggcaacgaa ctcaatgggc atccacgagg gtgatgcggg
     2221 tgcaagcgct ggcaatcaag gcattcagtt tgccaccgtg gctggtcgaa tctatgagaa
     2281 cgcacgaaaa ctcgatctag gccaaaagtt accaatggag atgtttctcc aggatatacc
     2341 gacttaagcc tagcccggat tattcatgct cagaggaaaa acgacgacac tgtgtcgatc
     2401 gaagggtgag cctggaggag tgaagacatc tagacgaaca gcttcaatga cgtctttggt
     2461 gcttgattcc cccgctcgca tttttcccag gcgttcgctc cacaaactct atttgcagcg
     2521 cccatcaagg cacaggccga tttggcttcg ctccggaggg ctcgcccttc gatcgccgtc
     2581 acgagtgaat aatccgggct agcgagctgc gcttttgtat ctagcagaca gctgcttgaa
     2641 aaagccgtcc ttttcaagtt gggtcatgaa cttagggtcg aaaaaatctt tcggtgttgc
     2701 cgttcgtgct ttgggctgtg tttctgagag cgcttcaagg atattgctca ggccgggctc
     2761 tgatgtcctc gggagatcct gcaagactct actgtagaaa tcataggtct cgtcgagaac
     2821 gcgacgatct tggacgcgtg tatatttctc caacactttg gtacccagct cgcggtcggc
     2881 tttaaacgcc cagatgcctt cgataagcgc tttcatcacg ttatgagtta cgcctggtct
     2941 ttgctgaacg tatttcatcg tacttaccaa actgttgttt acgaattgaa tttcgctccg
     3001 gctcaaatcc aggagctctg tcatgcccga tcgaatcgcc tgtgtagact gaggcggaga
     3061 gaagacggac gcttgaatta gtcccgattt taatgctgca aaggcttcac cgatgccgcc
     3121 cgtttgcacg atctcgacat ccttcgacgt aagtccccaa ctctttagag cgagcttggc
     3181 tgcataatca gttgcagcgc cgaagcgcgt gatcccaact cgcttgcccc tcagctcggt
     3241 aggttggcgt atcgaaggaa gggacacaaa ggacatcgtg agcctgttca cgaaagaggc
     3301 gaggatcacg ctatcagcgc cattgagccg agcgagcaca gcaggcggcc ctacgaccat
     3361 cgtaaaatcc gtgtcgccag caagcagtac ggacattggt agcgtaccgc ctccgatata
     3421 aatgaggctc ccattcacac cgtgtttttc gaaaagcgcc aaatctttgg ccatccaagc
     3481 aggagtgcta agtccactcg gtgcgctata gctgatacgc acggcattgg tttgagcatc
     3541 cgcacggcaa aacggattac taaaaattag cattaaaaca actgcggcac gcaaagattg
     3601 aagcgctagg gttttgaaag cgatttgttg catcagcggg tttggttcgt tgaattgatc
     3661 tgctcttatc gcatgcaacg aaacaatgtc aacgccgctg gatcgcatcg gccgtttcct
     3721 atttgcgttt acgccgatcg cacacggatt cacaagagga gcgctctatg ttgccaaatg
     3781 cgcccgtgcg ggttcaggca tgagttgttg actcagtctt gagccaacgc tgcagctccg
     3841 actcagatca cttatccatg ttctccacat aggccgcatc cagccgggtg atcatcgatg
     3901 cactcgcgat ggagatttga tcgccatctt attacggccg ccttccctga caaaaagagc
     3961 ttgacagtct accagattta cggctagcga atgtgatagc gctaaccaaa cgaggaacca
     4021 accatggcac gcaatggcta taagatcttc gatgcggaca cacatgttcg gcccgatgcg
     4081 gaact
//
LOCUS       DASUVS010000086         5197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1341330, whole genome shotgun sequence.
ACCESSION   DASUVS010000086 DASUVS010000000
VERSION     DASUVS010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341330"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..286
                     /locus_tag="VF182_04905"
     CDS             227..286
                     /locus_tag="VF182_04905"
                     /inference="COORDINATES: protein motif:HMM:NF027602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436447.1"
                     /translation="MHKKGVAFEVEFLTLGGRP"
     gene            complement(405..1667)
                     /locus_tag="VF182_04910"
     CDS             complement(405..1667)
                     /locus_tag="VF182_04910"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6436448.1"
                     /translation="MTWLRRIKTISPLKWRALESFNYREFRLLWYGQVFTAMATWMDS
                     IARGWLIYELTNSSFQLGLVRGVQAIPTLLLSPIAGSTADLYSRKTQILIAQVVDGLL
                     YAWVAVMIITGDIQPWHVYLTSLGLATVQAFQLPSRAAMVADAVPPSCLTNAMGLNAV
                     VFNSARSVGPALAGVLIALYGTAGSYSIQALFFLLATVWTIMLRPKQRLSRATNDPGE
                     HPESFRQNILEGWKFSWRNYEVRVGLLVVSAAMFLLIPFTTLLPVFARDILQVGARGQ
                     GILLTCMGGGALFSSVLVAFLGDRMPRGLVMIGGVAVYGILLVMFAASSSFVLSMALM
                     FFVGLCHVTSHALIQIVVQFYSPPEFRGRTMSIFHMTQVLLVVGAMFIGSLATFIGAR
                     WATAVLGVIGTSAMTAIYTLMPRARRIR"
     gene            2448..3458
                     /locus_tag="VF182_04915"
     CDS             2448..3458
                     /locus_tag="VF182_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436449.1"
                     /translation="MPKDRAKLRMLIAVCVCFFLLFTNALISEAAVPPNQLMITFGSL
                     SERETALFVARDYGIFAKHGLDVKLVHVRNGAVALSALASGDAQFYLGAATGSTLGAI
                     AGGLDAVFVTSLIHKLEGTFVVAPAIKSPAELRGKTVGVTSIGGGVWMRSMLAFEHWG
                     LEPQKDKINLRVVGDESLLAQAMPNGIIDGSYFNYTFASVLERQGFRVLADLAKLNIP
                     YQNTALLAQRRYLNAAPETTEKLLRAVSDAVIFVLEPKNKELVLKSLSKGLRLPRPED
                     ALGGYDRLANFYERRIFPNVEGIRSTIRFLGPGNEKIRALKAENLVDDRFVKKLEQEG
                     RF"
     gene            3547..3774
                     /locus_tag="VF182_04920"
     CDS             3547..3774
                     /locus_tag="VF182_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436450.1"
                     /translation="MADRISKLPRGRKIPHIDVVRARLRNHVDVVLASNNDTLIRMVE
                     RFFSETIDRLIQERPKGEIVPIKQDNPKSDK"
     gene            4054..4830
                     /locus_tag="VF182_04925"
     CDS             4054..4830
                     /locus_tag="VF182_04925"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6436451.1"
                     /translation="MKMISPVAESAYSKAVVAQYGTLLFISGQESIDQDGRLIAPGDF
                     NGQIQQVWNNMRAILEKAGGELDNVATMTVYTTERRWGRQFTGGRKEIFKQGFPASAF
                     VEARKLRTPGALLQIPAIAVLDERKGNMSESPEKIFIDPLVELGQSRAVKVRGGSMVF
                     LAGHTAGEMPNGSEAFNFTLQAKRTFDRIKASIESAGGRLSDIVSMSVFMTDMRHVSE
                     FQNVLRELFGVNLPASSYVEVTHLARPELLLEIQPIAIVA"
     gene            4839..>5197
                     /locus_tag="VF182_04930"
     CDS             4839..>5197
                     /locus_tag="VF182_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436452.1"
                     /translation="MQVLFLLTLLSVFFFSSAYGQSEDELVAGAKKEAKVTFWSGMAL
                     DDSRLVAAGFEKKYPFIKVDIFRAGGEQIVNRAIAEAGAGQNTSDVLNAFALKVLQER
                     NFLQPYAAAEAAAYPAA"
BASE COUNT         1248 a         1402 c         1447 g         1100 t
ORIGIN      
        1 ccaaatccga caccgaccga ttgaaaacag gggtgcgtaa cccgaggatc aaacctgagt
       61 tgaatcaacg ggagaagaaa aagaaattct tctccgcagg aaatctttgc ctacttgacc
      121 caccggctaa tgggtgaccc cttcttccga cccttcttcc ccgactaaag aaaaaagtgc
      181 cctgacgacc gggttgaaca ctggagatgt cggcaccgtt atccatgtgc acaaaaaggg
      241 ggtagcgttc gaagtggagt tcttgacgct cggggggaga ccgtagctat cgcaactttg
      301 aaagcttcgc aggttcgtcc ggttcaaaaa aagggaaatc accgacgcac gtcggttggg
      361 cgcatacatg ctctatgact taaccacgcc gttcttcatc cacgtcatcg tatacgccgc
      421 gcccgcggca tgagcgtgta gatggcggtc atcgccgacg tgccgattac gcccagcacg
      481 gcggtcgccc agcgcgcgcc gatgaaggtg gccaggctgc cgatgaacat cgcgccgacg
      541 actaaaagca cctgcgtcat gtggaagatc gacattgtcc gtccgcgaaa ctccggagga
      601 gaatagaatt ggacgacgat ctggatcagc gcgtgggagg tcacgtggca cagaccgaca
      661 aagaacatta acgccatgga cagcacaaaa gaggaagacg cggcaaacat cacgagcaat
      721 atgccgtaaa cagcgacgcc gccgatcatg acgagcccgc gcggcatgcg gtcgccgaga
      781 aacgccacca gcaccgaact gaacaaagcg ccgccgccca tgcaggtgag caagatccct
      841 tgtccccggg cgccgacctg gaggatatcg cgcgcaaata ccggcagcaa cgtagtgaac
      901 ggaatcagca gaaacatggc cgcggatacg accagaagac cgacccgcac ttcatagttt
      961 ctccagctga acttccagcc ttcaagaatg ttttgccgga aagattccgg gtgttcgcca
     1021 ggatcgtttg tcgcccttga taagcgctgc ttcgggcgga gcatgatcgt ccaaacggtc
     1081 gccagcagaa agaaaagcgc ttgaatcgag tacgagcccg ccgtgccgta caaggcgatg
     1141 agcacgcccg cgagcgccgg cccaacgcta cgcgctgaat taaaaaccac cgcattgagc
     1201 cccatggcgt tggtcaggca actcggcggc acggcatcgg caaccatggc cgccctcgac
     1261 ggcagttgga acgcctgtac ggtcgcgagg ccaagcgaag tcaaatacac atgccatggt
     1321 tgaatgtcac cggtgatgat catcacggcc acccaggcgt agagcaagcc gtcgaccact
     1381 tgcgcgatca ggatctgcgt tttacgtgag taaagatcgg cggtgctgcc ggcgatgggc
     1441 gataacaata atgtcggaat tgcctggacg ccgcgcacca gtccgagctg aaaggacgag
     1501 ttggtcagct cgtagatcag ccagccgcgc gcgatggagt ccatccaggt ggccattgcg
     1561 gtgaacacct ggccgtacca gagaaggcga aactctcggt agttgaacga ctcgagagcg
     1621 cgccatttga gtggagaaat cgttttaatg cggcgcagcc acgtcatatg cgggcggatc
     1681 tgctaaggtg gagccgacaa agatccgcgc cgcagtcttc tttcgataat ttcatttgtc
     1741 tgaagacggc aagctcgctg atatggaatg ttccggcagc gatcggtatc tggagttgcc
     1801 gagaaaatgc gttgcatcct cttgcgcgat ttagaatcat ggtgaacccg ccattgtcac
     1861 ttctttggga ctgtagttct ccagcgctgc atctactgcg accatcgcta gaaatcaata
     1921 ctcccagtct taaaagaaag tgttcacaac ggatattcgc taggtttatt ccttcgcgta
     1981 aaaacacgca gcgcgcttgt acgcgttgac ggtgaacctt acacggcgaa tttcacagac
     2041 gaattgaggt acatcggcgg cagaatcgcc gtcgccgcgg aactcgcgca atcaaaggtg
     2101 tgagaggcgt cctgggctgc gtctggtcat gtccctcgtt gtcgttgaaa atcgattctt
     2161 cggtattgat gcctgaaaat atttcttcca cagtgcctta taacgaacga tctccaatca
     2221 gccaacgcgt gagcatccgc cttagccggc ctttagcgat gaaacttctt taaggctttg
     2281 ccatccatcg aaagggcgca tcgtggtctt gcaacgggcg aaacattcgt caggaagtac
     2341 gaagtgatcg accgtgagag cgaactccac tatagaagcc cggctcagac cgggatataa
     2401 ccgccgctga ggaaaagata tcaaagtagg agttccgtgg aggatttatg ccgaaggacc
     2461 gtgcgaaact gcgtatgcta atcgccgttt gcgtttgttt cttcctatta tttacgaatg
     2521 cgctgatctc cgaggccgcg gtcccgccca atcaactaat gatcaccttc ggcagcctca
     2581 gcgaacggga aaccgcgctt ttcgtcgctc gcgattatgg aatctttgcc aagcatggct
     2641 tggacgtgaa actggttcac gtgcgcaacg gcgccgtcgc gctttcggcc ttggctagcg
     2701 gagatgcgca attttatctg ggggccgcca ccggttctac tcttggcgcc atcgccggcg
     2761 gtctggacgc ggtgttcgtc accagcttaa tccataaact ggaaggcacg ttcgtcgtcg
     2821 ctccggcgat caaatctccc gccgagttaa gaggcaaaac ggtcggtgtc acgagtatag
     2881 gcggcggggt ctggatgcgc tcgatgctgg ccttcgagca ttggggcttg gagccgcaga
     2941 aggataaaat caatttgcgc gtggtcggcg acgaatcctt gctggctcag gcgatgccga
     3001 acgggatcat cgacggctcc tatttcaatt atacctttgc atccgttctc gagcgccagg
     3061 gcttccgtgt gctcgccgat ctcgccaagc tcaacattcc ttatcagaac acggcgctct
     3121 tggcgcagcg gcgatatctc aacgctgctc ctgagacgac cgagaaatta ttaagagctg
     3181 tgtccgatgc cgtgatcttc gtcctggagc caaaaaacaa agagctggtt ctcaagagct
     3241 tgtccaaagg actgcgtctg ccgcgtcccg aggacgcgct cggcggttac gaccggctcg
     3301 cgaattttta tgaacgccgt atctttccca acgtagaagg aattcgttcg acgatccgct
     3361 tcctcggacc cggcaatgaa aagatccgcg ctctgaaagc ggaaaacttg gtcgacgata
     3421 gattcgtaaa gaagttggaa caggaaggcc gcttctgagc cgacgcgccc agcatccgcg
     3481 agttctcgat caaaaaactt catctaggtt cttctttcgg taggacggat ctgatggaat
     3541 agaactatgg ctgaccgtat ttcaaagctt ccccgcggga gaaagatacc acacatcgat
     3601 gttgtccgag ccagattgcg caatcacgtt gacgttgtcc tggcgtccaa caacgacacg
     3661 ctaatccgga tggtcgagcg gtttttttcc gaaacaatcg accggctgat ccaggagcgt
     3721 ccgaaaggcg agatcgtccc gatcaagcaa gataatccga agagtgataa gtaagtacga
     3781 tgcgtgtcag ggcgctgtac tcgctcgcgc tggcttaccc actaaatcgg tgctgtcggc
     3841 gtaagttgga caacgattca caatgaatcc acctcatgaa gccagcggat aaccaagacg
     3901 actgacacaa aagacatcgt ttggctccgg ccaactttcc cgtttcagaa agccgcaaat
     3961 ggaccgcttc cacgattcat tccgatgcgg caatcttgcg aggtggtctc gtcccttgtt
     4021 atataggctg atttagtgaa ataggacgcc tcgatgaaaa tgatttcacc ggttgccgag
     4081 tctgcgtatt ccaaagctgt cgtggcacag tatggaactc ttttgtttat ctctggacag
     4141 gagtcgatcg atcaagacgg aagactgatc gcgcccgggg actttaacgg acagattcag
     4201 caggtctgga acaacatgcg agccatcctg gagaaggcag gcggtgagct cgacaacgtt
     4261 gccacgatga cggtttacac caccgaacgt cgctggggcc ggcagtttac cggtggtcga
     4321 aaagagattt tcaagcaagg ttttccggcc agtgcctttg tcgaagctag aaaactccgc
     4381 acgccgggag cgctactgca gattccggcc atcgcggtct tggatgaaag gaaagggaat
     4441 atgagtgaat cgcctgaaaa aattttcatc gacccattag ttgaacttgg gcaatcacgg
     4501 gccgtcaaag tgcgcggcgg cagtatggtc tttcttgccg gtcatacggc cggagaaatg
     4561 cccaacggca gcgaagcctt caattttacg ctgcaggcga agcgcacgtt tgatcgaata
     4621 aaagcctcga tagaatcagc tggcggccgg ctatccgata tcgtcagcat gtccgtgttc
     4681 atgaccgata tgcggcacgt gtccgagttt cagaacgtgc tcagagaact cttcggcgtt
     4741 aatttgccgg cgagctcgta cgtggaggta acccatctgg ctcgacctga gcttttgcta
     4801 gagattcagc ccatcgcgat cgtagcctag gagaacggat gcaagtactc tttcttttaa
     4861 ctcttctatc ggtatttttc ttcagcagcg cttatggcca atccgaggat gagctcgtag
     4921 ccggagccaa gaaggaggcg aaagtcacct tttggagcgg catggcgctg gacgactcac
     4981 gcttggtggc cgccgggttc gagaaaaaat atccgttcat caaggtcgat attttccggg
     5041 cgggaggcga gcagatcgtc aaccgcgcga ttgccgaagc gggcgccgga cagaacacat
     5101 ccgatgtgct caatgctttc gctttgaagg tgctgcaaga aagaaatttt ttacaaccgt
     5161 acgccgccgc cgaagcggcc gcttatccgg cggccta
//
LOCUS       DASUVS010000087         6440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1122526, whole genome shotgun sequence.
ACCESSION   DASUVS010000087 DASUVS010000000
VERSION     DASUVS010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122526"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VF182_04935"
     CDS             <1..534
                     /locus_tag="VF182_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436453.1"
                     /translation="NVSVSAVAVNQALPGLPIVVAESTCGMDTILDLQKGGKGNAGWE
                     TYQIINASTDEVRGLFEKLPLCAGQPAIDVGYCGNVANGVNATIFNEHLKPMFKADPN
                     RCYLLPVVKNGGNLNQCQMITDWARFCPDKGNNTDDAFPGPGLLKGRVTCGQSVHESR
                     DSQCFVQRLVRDKTSGM"
     gene            971..1237
                     /locus_tag="VF182_04940"
     CDS             971..1237
                     /locus_tag="VF182_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436454.1"
                     /translation="MSDLLSLFGVITVTFYIGMLLSPAFAAGKLKTPHASWILWTGLL
                     AGTMCLVFVSYLKMPVVFFAWVGLMVWAGIDVQIGQAKRMDSLG"
     gene            complement(3103..3249)
                     /locus_tag="VF182_04945"
     CDS             complement(3103..3249)
                     /locus_tag="VF182_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436455.1"
                     /translation="MAHSPPGKKAQELELSEGQVLERSLELPLVGLVRELQLAEFWGW
                     VLVL"
     gene            3839..4159
                     /locus_tag="VF182_04950"
     CDS             3839..4159
                     /locus_tag="VF182_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436456.1"
                     /translation="MKKLPLRVALTAVIAICALGFIASPAFSKQEGKLKPKGSEKSVT
                     RTSSGREAGELPSGIERYSERKGNLPSGLQKKKDADGPLTHGLDAGGKKLDSTAKGKK
                     VSKK"
     gene            4296..4724
                     /locus_tag="VF182_04955"
     CDS             4296..4724
                     /locus_tag="VF182_04955"
                     /inference="COORDINATES: protein motif:HMM:NF023514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HEX6436457.1"
                     /translation="MQQNSTRAFLLVGLMVLNLNGCAAPLALTLLGVGAGVTTGTAVS
                     YTMDGIAYRTFTASLPQVERATLTALNDMGIKIEGTDKTDEGKAIKAKGLDRQIEIEL
                     QAISPKTTRIRTVAKEGVFFKDRATATEIILQTERVLGKA"
     gene            4741..5580
                     /locus_tag="VF182_04960"
     CDS             4741..5580
                     /locus_tag="VF182_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436458.1"
                     /translation="MKFLHHFSIAVSLLIMTPAPNFAQTSSGSSTPATNISNEYSYFL
                     KNVDSKQVVNDLRNGQWTSTTTDPTTNATTTSTEALPTGKMGFGNVKISLALAQESLR
                     QQGIMQPTSEQLHTALVGGQTVPGDSTTMTNGILQMRSEGMGWGQIAQKYDVKLGQLM
                     SGKQPASTSNTSTTPKTTTSKGITTASGNSSTTAMGKGNGKTFSGSESQGKGIVSASG
                     QTSGASFSSGSQSGRGIVSGSGRAMGAMNGVVTGAGSGGGNQYGASGGGNGNGGGHGK
                     GPK"
BASE COUNT         1576 a         1620 c         1690 g         1554 t
ORIGIN      
        1 aacgtgagcg tcagcgcggt tgctgtcaac caagctcttc caggactacc gattgtcgtc
       61 gcggaaagta cttgcggcat ggatacaata cttgatttac agaagggagg taaagggaac
      121 gccggctggg aaacttacca aataattaac gcgagcactg atgaggtgcg cggcctattc
      181 gaaaagctgc ccctgtgcgc aggccagccg gcaatcgatg tcggttactg tggaaatgtg
      241 gccaatggtg tgaacgcaac gatctttaat gagcacctaa agcctatgtt taaagccgac
      301 cctaacaggt gctacttact tccagtagtc aagaatggtg gcaacttgaa tcagtgccag
      361 atgatcacag attgggcacg gttctgcccc gataagggga ataacacaga cgacgctttc
      421 ccggggccgg gtttgctcaa gggcagagtt acttgtggcc agagtgttca cgagtcacgg
      481 gatagtcaat gtttcgttca gcgcctggtg cgggataaaa cgtcagggat gtaagtattc
      541 ggtgtgtttt agccaagccg agcttaggtc ggcactgcac cacgacgggc accagaccca
      601 atggagccat cccgggtttc gcctgagaga agctgaatgt tggtcattat tggactcgcg
      661 cgcgctgatt acgaccagct gctacataac gtctctcccg aggcacccgc atttaatgtc
      721 ctccgcggct ccctgaactc gctcgacgtg aagaaccgct gttcaagtgc gtaatcattg
      781 agtgcaatcc cgatgatgcc aacactactc ttggaagccg cgaagccgca ttgtacggag
      841 gccgtgccaa acattgagta cggatttaaa ttatcacgta gcttcgctgt gttccgttaa
      901 tcatctgctc aagcgccgta aggagctttc ctacaaagcg cgagcgtaaa acgttacctg
      961 ggagaaagtt atgagtgacc tattatcctt atttggcgta atcaccgtta ccttctatat
     1021 cgggatgtta cttagccccg cttttgccgc cgggaagctc aagacacctc atgcatcgtg
     1081 gattctttgg accgggcttc tggccggcac catgtgcttg gtgtttgttt cttacctcaa
     1141 gatgccggtg gtgttcttcg cgtgggtcgg cttgatggtc tgggctggaa tcgacgttca
     1201 aattggacag gcgaagcgaa tggactcttt gggatagcgc cgggtgatcc gcgctgtatt
     1261 gctgatgctg gagcgtcatc acaggcgctg agtgcaaaga aatcgatatg ggaggagggt
     1321 gctgactatc agcgcatact actcccgacg caatgaacct ggcttaagca ggccgtgacc
     1381 atctgcgctg gtatgccgcc gaaaagcagt tcggcagttg gggcaaggcg ttatatgctg
     1441 tcgggatcag tcccgatctc tattacgttc gtccaaattg cgcggccaaa actgacccac
     1501 gagatcgtcc ggctatggca acctacctcg gtcgctcgtg tccgcgatgc aatacttacc
     1561 ttgggcattg tcttacgcga gccgggacgt aacgtcccgg ttcgagctgc aacgggcact
     1621 gcctcaaatg cgattacgac tggcttggat catcattgaa gggaagcggg ctgtatgcta
     1681 gatcacaaac aaaactattg aacgcaacgc agtttgaaac gcatcggtga attaaacgct
     1741 gagatcgcgc agccgttgta ggatactttc atgatttcaa tctcgtcttc tagactcttt
     1801 atcacttcag acgagctctc gaacattcga atttcagtac atggaactcg gtggagatta
     1861 tgctcaatga attggcaaaa tcgtgtgcga ggctctcgag ctgatttctc gaagtcacgg
     1921 tgtgacagcc ataagcatgc cacattcaaa agactagatt gctactccaa taccgcagag
     1981 ggcgtctaga tactgcgccc cttcagcgac ccggggccag aaatatatct tctcattatc
     2041 cttctgggtg tgcatcttcc aagcttcgac aatacaaccc tgatgtttta gcgcgtgtat
     2101 tcttgtaggg ggatcattat tagagaatgc tcgtgcgata ctttgttcga cgagttcacc
     2161 gcattaggtg actgtagttc cccatcgtat ggttatcact acgcgcatgg cacacgccgc
     2221 cgacagttcg gcgcgtttct agtggcggca atggtaagtt gctcactgca tactttcagc
     2281 aaacccaacg atgaagggga tcctcttaga cccggcacag atgcgcacat cctgaaagta
     2341 tttcgcatga tttgacgatg atcgagcgat cacgatatta agaaactacc gccgcgcgct
     2401 gctgacaagg cacggcctcc tggtaattga accggtcatt ttgaggggtg atcaggcgcg
     2461 tttagtgagt tttgcttcca agttgaattg actccatgcc ctcgcggcgg cgccatttcc
     2521 ctggcgatgg ctgcttgccg tcaccttggc ctctgctcat ccgagccgga gatcaaccgc
     2581 aagttgaaaa tggccgagca attttgcggt agagaatgcg gaggttcagt acgcccaccg
     2641 ggatgaaatc tcagagttcg tttgaagagc gcttttggcc atcactgcgc agataactcc
     2701 aggatcccgg cggtgcgtcc ttctcagagc atgtgaatcg aaaccgctgg gggtttcatc
     2761 cattttaaca tttgctaccg gccataggcg cattccttag aacagatagc caataaaaat
     2821 tctgttcggc gcgaggttct ccaccagcac tactgcattt taacgagcga gcaggacccg
     2881 tgaccactca ctcagtacaa gcaggagcaa agctgcgggc agtgctaaat gagcttatga
     2941 gataaaacgt gggccgttct tacccatttc aggtttagta ttctcgctgg cgcttcatgc
     3001 gctgcagctc tagacgttga cgctcgactt cagcctggtt cgcttgaatt tgttgctgtt
     3061 gctgctggtt aacattttca tgaccttgga gctgatcgcc gactaaagca ccagcaccca
     3121 gccccagaac tccgccaatt gcagctcccg caccaggccg accaacggca gttccaatga
     3181 gcgctccagc acctgcccct ccgacagctc caattcctgc gcctttttcc cgggtggtga
     3241 gtgcgccacc cgaacagccc gttatgacga gcaatgtaat taggaacagc gatcttctgc
     3301 aatgtagact tagctttgat cttctcatac ttgtcacagg ctccttgcgc tgattcttag
     3361 ttcccctggg ttggtaactc caatgaccgc cgagccgagt gagccgtaga gtggacgtgt
     3421 gccgtaccga agttacagcc agtcccggct cttagcttga atctgtttgc gatgttctcg
     3481 cgcatcggct caccggagtt accctctcgc atataagcgc aaggaaaatg ccagggtaga
     3541 atccgacgtg tgttggcgct tgattgattt tgcggaaaca ttaccggctt accttaggat
     3601 ctgacgatag aaatcagtgc gcccgtcgat acatccgaac gaaaacttgc caggcaaaac
     3661 tttggggaca acggattcaa cactgtgcat attttcctaa atccgaaata tttccctgtt
     3721 aacgcaaacg catgacgctt ctaagtcgtg gtaatctttg aatccgataa atggcatgtt
     3781 tgctgctgaa cagttcgagc gcgtagctgc tgctagacaa cagactacag gagaaacgat
     3841 gaaaaaatta ccactacggg tcgcattgac ggctgtgatc gccatctgtg ctctcgggtt
     3901 tatagcgtct ccggcttttt ccaagcagga gggaaaactt aagccaaaag ggagcgagaa
     3961 gagcgttacc aggacaagta gtggtcgtga ggctggtgaa ctgccttccg gtatcgagcg
     4021 ttatagcgag agaaagggca atcttccctc aggactgcag aaaaagaaag acgccgatgg
     4081 gccgcttacg catggtcttg atgctggtgg caagaagcta gactcgaccg ccaaagggaa
     4141 aaaggtttcg aaaaagtgat tgtgaattgc ttgaatgctt cccgagagag caagcaacaa
     4201 ttcacgactt taatacgccg acctgggttc ggtttaatag taaaccttaa cacagcctaa
     4261 gtagcaggcg ataatctgtt ttttaggtgg ttagcatgca gcaaaactca acgagagcat
     4321 ttttactggt tggacttatg gtgctcaatc tgaatggctg tgctgctccc ctggctctga
     4381 ctcttcttgg agtcggtgcc ggtgtaacta cgggtacagc agtttcatac actatggacg
     4441 gtatcgccta ccgaactttt acagcgtccc ttccgcaggt cgagcgggct acgctaacag
     4501 ccctaaatga catgggtatt aaaatcgaag gcacagataa gactgatgag gggaaagcga
     4561 taaaagcgaa gggactcgac cgtcagatcg agattgaact gcaggccatc agccccaaga
     4621 ccacccgaat ccgcacggtt gccaaggaag gagtattttt caaagaccga gcgaccgcga
     4681 cggaaatcat attacagacc gaaagagttc ttggaaaagc gtaaatacag aggaggaatt
     4741 atgaagttcc tacatcattt ttcgattgcc gtttcgctat tgatcatgac tccggcaccg
     4801 aactttgctc agacatcttc cgggagtagt acgcctgcga ccaacatcag caatgagtac
     4861 agttactttc taaagaacgt tgactcgaag caggtcgtaa acgaccttag aaacggacag
     4921 tggacgagca ctactacaga ccctacgacc aatgcgacga cgacaagcac cgaggctctt
     4981 ccaaccggga agatgggatt tggaaatgtg aagatttcct tggctctagc ccaggagagc
     5041 ctaaggcagc aaggcatcat gcaaccgaca tctgagcagc ttcataccgc actggtgggc
     5101 gggcagactg ttccggggga ctccacgacc atgaccaacg gcattttgca gatgcgcagc
     5161 gaaggaatgg gttggggaca aattgcgcaa aagtatgatg tcaaactagg tcagctcatg
     5221 agcggaaagc agcctgcgtc gacgtcaaat accagtacga cgcctaagac tacgaccagc
     5281 aaaggcatca ctactgctag tggaaactcg tcgactacag ctatgggtaa aggaaacggc
     5341 aagacctttt ccggaagtga atctcagggc aaaggaattg tcagcgcctc gggccagacc
     5401 tccggtgcat cctttagttc aggcagtcag agtgggcgcg gcatcgtgag cggttctggt
     5461 cgagccatgg gtgctatgaa cggcgtcgtt actggggctg ggagcggcgg tggaaatcaa
     5521 tatggcgcat cgggaggcgg taacggcaac ggcgggggac acggcaaggg ccctaagtaa
     5581 agtcatcgcg tatagcgcgg aaggaaaaac ctaatgccgc tgtcataaat taccggcacc
     5641 ggcgattgcg ttgtcatgag cgtgcccgtg atattactaa tttttggatc ttgaaattcg
     5701 ctctgcgtga atcgaacgat acaacactag aatcctcacc tcgtgtcgtt ttgccgattt
     5761 tctctaacgg tgtaataggt cggaatccaa gatcccgagt atttgtttct cttctccctt
     5821 gtacggttta gacatgcggg acttgatgtc gagcaactgc caaaaatatt tctcgcaccg
     5881 atagtcctga ctccatcgag ctttttggct cgacgtacat actagaagtc taagttgttg
     5941 ccagttctaa gcgaaccttt caaagacatt atcagcgtcg ctttcttcac cctcggcatt
     6001 ttcatgtcga cagattttta aactcttgcg tttttttgac gccaccggct agcgcgccgt
     6061 tgtctcattc aacgatggtc ggccacgcag cccgtcccgc gcttagcgcc ggatttattc
     6121 gtcttgacgc gtcaagggct tgtcctgcag atactctcca agtcgtttga aacagaaccg
     6181 cgccattggt ggccgaaccc caaactggcc atgttcgatc ctcatcggaa aggttgattt
     6241 gatcgtttac ccgctggtat gcgccgcgcg atcaaggtgc ataacatggg ttcatcggat
     6301 atgtttttta ttgcttaact ccagatttgg cgataagcgt tgggcatccc aagcacatat
     6361 tcgccgagcc ggaatctact gacgtgctat atcaactgcg gttcaggcgt ggagaaaaat
     6421 gctacaaacg attctggtcg
//
LOCUS       DASUVS010000088         8494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_903997, whole genome shotgun sequence.
ACCESSION   DASUVS010000088 DASUVS010000000
VERSION     DASUVS010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903997"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VF182_04965"
     CDS             <1..371
                     /locus_tag="VF182_04965"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEX6436459.1"
                     /translation="DTRDEASSSNQDAADSIQTKATEDPTSQASGKAEDQEAVLPEIN
                     FSTFIISLSTQALMHLGEIANPLTGKVEPDRLVAKQMIDIIGMLREKTRGNLNSGEDR
                     LIEDILFDLRMKYVEAVKKK"
     gene            381..1880
                     /locus_tag="VF182_04970"
     CDS             381..1880
                     /locus_tag="VF182_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX6436460.1"
                     /translation="MDIKRVWSGSFTLKGVAIAATASLLLGLAISGSFDWLAPSRAVN
                     MGESASSDIRTANQLPDFVALSKKLRPVVVNISSTQVSESRGQQGPQEFGSPFGEEDP
                     FNDFWRRFFGGPTPRGPQRQRSLGSGFIIDGDGSILTNNHVVENAQKIVVKLADEQEY
                     EAKVVGRDPKTDIAVIKIEAKTKLTPANFGDSDGLEVGEWVMAIGNPFGLDSTVTSGI
                     VSAKGRHIGQGPYDNFIQTDASINPGNSGGPLINLRGEVIGINTAIFSRTGGNIGIGF
                     AIPVNLVKELLPQLRGKGKVTRGYLGVLIQKVTPELAESLGMEKSQGALVANVSKDGP
                     ADKSGVKVGDVIVEFDGKEIKDSGDLPIIVARTPVDKKVRMKVLRDKKELTLNVAVGE
                     LKEEEVVASAPEKGEMGLTVQRLTPQIAESLGLEKTEGIVVSAVEPGSAADEAGIRRG
                     DVIVEIDRKPIRNLEEYKKSIGTIRKGKGVLFLVRRGESTLFLALRPQG"
     gene            2001..4316
                     /locus_tag="VF182_04975"
     CDS             2001..4316
                     /locus_tag="VF182_04975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX6436461.1"
                     /translation="MKKSNLRFFSISLIVLLLITSSGIVIGSWYLRTLEDTVTAKFDG
                     RKWRFPSKIYSDSYLLYAGMSLRTEDFLEKLRRLGYHETKAKPDGKGEYRFQRSSGLL
                     EIYLHDFSYPTETFKGVPIRMSLQGNTVGKLQNASSGEELFSLELEPELVTGLYETVW
                     QERRLVKLPEVPPLLVRAILAIEDERFYRHFGVDPVALMRAMWVNFRSGAVVQGGSTL
                     TQQLVKNFFLGSERTMSRKLKEAFMALIVERNYSKNEILENYLNEIYLGQKGSQGIYG
                     VWEASQFYFSKPLTELSPGEMALLAGLIRGPNNYSPYRNVEAATRRRNVVLAKLLDDK
                     IITRRQYEASLSETLPHRELIKVTNDAPYYVDFLRRELAENYSNDVLTTEGLRIFTSL
                     DLHLQKLAERSLSEGLKRLEAKYPGLRRRGQEDSLEGAVVVIRPQTGEIKAMAGGRNY
                     QKSQFNRVFQAKRQPGSIFKPFVYLAALMYGAENGRKFTAMTKVDDSPFTWEYDRQEW
                     KPGNYNDEFFGTVTLRRALEKSLNSATARVAREVGIRRIRDLAQRLGIQSSLPAVPSL
                     ALGAAEVTPLEVAVAFSTLANNGVRTRPLTVKQVLAPDARVLEKRDVRVEKVVSAQMA
                     FLINHLLKGVLDRGTADSARRAGFTRPAAGKTGTTNDYKDAWFVGYTPDLLTVVWVGF
                     DNQSKLGLSGSQAALPIWTDFMIRATAGTPMTDFVAPPGIKFVDVDPISGQLATASCT
                     SVIREAFIEGEEPALTCALHPGEQMQAPRLE"
     gene            4364..5110
                     /locus_tag="VF182_04980"
     CDS             4364..5110
                     /locus_tag="VF182_04980"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6436462.1"
                     /translation="MAIAMVLGLCILAVQSCAPQRPYRRPVPTAAAKPQPEPAPIPSP
                     PTSAPVSELSNRSIPPDPKIREQDLKANEKVLQPSITTSKEAPPPIAPPETPPPAKPP
                     LVDDSSLLAKIIPGTPPQRAASLRLSEEARKLLESGDYSRALSRLERAIAIDSTNAYG
                     HFLLAKTQFGLKHYQDSLNFLDVAESRLGGEPFWLAEVYALKGENYRALGMAERAEES
                     YAKALAINPDNRTATEALFHKQSGTQPAQR"
     gene            5121..6119
                     /gene="tsaD"
                     /locus_tag="VF182_04985"
     CDS             5121..6119
                     /gene="tsaD"
                     /locus_tag="VF182_04985"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX6436463.1"
                     /translation="MLVLGIETSCDDTAAAVLLDGRTILANVVSSQDEIHGAYGGVVP
                     ELASRQHIRNILPIIDGALGKAGVGVRDLDGIAVTHGPGLVGSLLVGLSVAKGMAFRW
                     GTPYVGVNHLEAHLLAIHLEHEVSFPYIALLASGGHTLLYRVQNVGDYHYLGGTRDDA
                     AGEAYDKVAKMMGLGYPGGRVIDSLAKTGDRNAIRFPRARLKKSPYDFSFSGIKTSVL
                     QYLKAQDHQAWKAHVPDIAASFQEAVVDMLVSPTVSAALAQNINRIVLAGGVAANSRL
                     REKISERAATKDLQVYFPSAKFCTDNAAMIALTGHHWIKGGRRDDFALNAHADLTL"
     gene            6164..7069
                     /gene="rsmA"
                     /locus_tag="VF182_04990"
     CDS             6164..7069
                     /gene="rsmA"
                     /locus_tag="VF182_04990"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX6436464.1"
                     /translation="MPTLYEEVRAALRESEFRPSKRLGQNFLVHEHVLQAIVDLLALK
                     TDDLVLEIGPGLGALTRRLVDRAGAVWAVEVDPFLIRRLQQSDLFADPKFHLISGDIL
                     KVPLAGILPKDKIKLIGNLPYSISTPVLFRVLELRDYFSSIVLMVQKEVAERIASAPG
                     TKAYGTLSVWCQIHGQVMQKIQVSPEAFFPRPKVRSTVLKMELYPRPRVPAQEMQVLR
                     GLLRAAFGQRRKTLGNVIGKWLKTGRDDVEEFLGSQNIDPQRRGETLSIDNFIDLTHA
                     LASSHLLRALSADASADHDRPQIDC"
     gene            7075..7896
                     /gene="mutM"
                     /locus_tag="VF182_04995"
     CDS             7075..7896
                     /gene="mutM"
                     /locus_tag="VF182_04995"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX6436465.1"
                     /translation="MPELPEVETVCRSLRPHLLHRTIERVETLETRLRVPVDELALAG
                     LAGKKIYEIRRAAKYILISLSDESVWVFHLGMSGKLVCVGADAPRRKHDHIIVHLVGG
                     GQLRYHDPRRFGLTLVTRKEILHELPQLRLLGIDPFDPDLTGEYLFRHTRSSDRRIRD
                     LLLDQRIIGGLGNIYANEILASVGIKPTIRACRLTRKQVYAIAQGVPQLLQQAIRWCG
                     TSFSDYRTADDERGAFQSHLRVYDREGQKCRACGCIIKRVAIGNRSAFYCPSCQK"
     gene            complement(8096..>8494)
                     /locus_tag="VF182_05000"
     CDS             complement(8096..>8494)
                     /locus_tag="VF182_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="band-7 C-terminal domain-containing protein"
                     /protein_id="HEX6436466.1"
                     /translation="REKRALILTSEGQRDAAINSAEGEKQQVIKASEANKQQQINEAE
                     GEAAAILAIAEATADGIRKVAESIQQPGGFEAVQLRVADNYLTQFGKLAQEGNTMILP
                     ATLSDVGSMISLAMNVIKQKGGNNGAPDKA"
BASE COUNT         1933 a         2293 c         2371 g         1897 t
ORIGIN      
        1 gagacacccg ggatgaggct tcttcatcta atcaagatgc tgcggacagc atccaaacta
       61 aagccaccga agatccaacc agtcaggcct cgggcaaagc cgaagaccag gaagcggtgt
      121 tgccagaaat taatttttca acctttatta ttagccttag cacacaagcg ctgatgcatc
      181 tcggtgagat tgccaatccg ctgactggaa aggtagaacc ggaccggctg gtggcgaagc
      241 agatgatcga tatcatcggc atgttacgcg aaaagacgcg cggtaatctt aactctggcg
      301 aagatcgact gatagaagac attctattcg accttcgaat gaaatacgtc gaggcggtaa
      361 agaagaagta ggagccgttg atggacatca agagggtctg gagcgggagc ttcacattaa
      421 agggcgttgc cattgccgca accgcttcac tactgctcgg cctagctatc agcggcagct
      481 tcgactggct cgcgccaagc cgcgccgtca acatgggaga aagcgcaagc tctgacatcc
      541 gaaccgccaa tcagctacct gatttcgtgg ctctttcgaa aaaattgaga ccggtagtgg
      601 ttaacatatc ctcgactcag gtgagcgaat cgcgcggtca acaaggcccg caagagttcg
      661 gcagcccatt cggcgaggaa gatccgttca acgacttctg gcggcgcttt tttggcggtc
      721 cgactcctcg cggcccgcag cgccagagaa gccttgggtc gggattcata atcgatggcg
      781 atggttctat tcttacaaat aatcacgtcg tcgaaaacgc gcagaagatt gtcgtcaaat
      841 tggccgacga acaagagtac gaagccaaag tggtcggccg cgatccgaag accgacatcg
      901 ccgtgatcaa gatcgaggca aaaacgaagc ttacgccagc caatttcggc gactccgacg
      961 ggcttgaagt cggagagtgg gtgatggcca tcggcaatcc tttcggcctc gatagtacgg
     1021 taacatccgg tattgtcagc gccaaaggcc ggcatatcgg ccagggaccc tatgacaact
     1081 tcattcagac ggatgcctcc attaatccag gaaactccgg tggtccgctg atcaatctgc
     1141 gcggcgaagt gatcgggatc aatacggcaa tcttcagccg cacgggcggc aatatcggga
     1201 tcggtttcgc gatcccggtc aatctggtca aggaactgct gccgcagctt cgcggcaaag
     1261 gaaaagtgac tcgcggctac ctcggtgtgt tgattcagaa agttacgccg gaattagcgg
     1321 aaagcctcgg gatggagaag agtcagggag cactcgtagc caatgtctcc aaagatggcc
     1381 ccgcggataa gtccggagtt aaagtaggtg atgtcatcgt tgagttcgac ggaaaagaga
     1441 tcaaagactc gggcgactta ccgattatcg tggcacgcac acccgtcgac aaaaaagtca
     1501 ggatgaaggt tttgcgtgat aagaaagagc tcactctgaa tgtagccgtc ggggagctca
     1561 aggaggaaga agtcgtcgct tccgctccgg aaaaagggga gatgggccta actgtccaaa
     1621 ggctgacgcc gcaaatcgcc gagagtctcg gtttggagaa aaccgagggc atcgtggttt
     1681 cagcagttga accgggtagc gctgccgatg aagctggcat ccgtcgtggc gatgtgatcg
     1741 tcgagatcga tcgcaaaccg atccgcaacc tcgaggaata taaaaagtcc atcgggacga
     1801 ttcgtaaagg caaaggagta ttgtttttgg tgcgccgcgg cgagagcacg ctatttctcg
     1861 cattaagacc gcagggctga ttctcttcaa agatcattgc actaactgaa gagggctcgg
     1921 gtggaatgtc atccggccct ctttttgttg atttttcctc cgatctgagt gaatattaac
     1981 ctgtcttcgg agtcgaccgt ttgaagaaat cgaatcttcg cttttttagc atctccctaa
     2041 tcgttcttct tctcatcaca tcgagcggca tcgtcattgg cagctggtat ctcagaactc
     2101 tggaagatac ggttactgcc aagttcgacg gacggaaatg gcgttttcct tcgaagatct
     2161 actctgactc atacctgctc tatgccggca tgagccttcg caccgaagat tttctcgaaa
     2221 aattgcgccg tctcggttat cacgaaacga aggctaagcc agacggcaaa ggcgaatatc
     2281 gttttcagcg atcttccggg cttttggaaa tctatctgca cgattttagt tatcccacgg
     2341 aaacattcaa aggcgttccg atccggatgt cgctgcaggg aaacacggtt ggcaaactgc
     2401 aaaatgccag cagtggggaa gaactatttt cgctcgagct ggaacccgag ttggtgaccg
     2461 gtctctatga gacggtttgg caagaacgcc gattggttaa gttgccggaa gtgcctccgc
     2521 tactcgtccg cgccatcttg gctatcgaag atgaacggtt ctatcggcac ttcggagtgg
     2581 atccggtcgc tctcatgcgc gcaatgtggg ttaattttcg cagcggcgcg gtggtccaag
     2641 gcggcagcac cttgactcag caactggtga agaatttctt tctcggcagc gaacgcacta
     2701 tgtcgcgcaa gttgaaagag gcttttatgg cgctcatcgt tgagcgcaac tactccaaga
     2761 acgaaatcct cgagaactac ctcaatgaaa tttatctggg gcaaaagggt tcccagggaa
     2821 tttacggcgt ttgggaggct tcccaatttt atttttccaa acctctgacc gaactgagcc
     2881 ccggtgaaat ggcgctgctg gcgggactta tccgcgggcc gaataattac tctccctatc
     2941 gcaacgtgga agcagcgacc cggcgacgaa atgtagtcct ggcgaagttg ctcgacgaca
     3001 aaatcatcac acgccggcag tatgaggcct cactcagtga aacattgccg catcgggagt
     3061 tgatcaaagt caccaatgat gcaccatatt acgtcgattt ccttcggcgc gagctggcag
     3121 aaaactattc taacgatgtg ctgaccaccg aaggactgcg gatctttacc agcctcgatc
     3181 tgcatctgca aaagctcgcc gaacgatcgc tttcggaggg gctgaaacgg ttggaagcaa
     3241 agtatcctgg cctgcgccgc cgcggtcaag aggacagcct tgaaggagcg gtcgtcgtga
     3301 tccgcccaca gaccggggag atcaaggcaa tggccggtgg gcgcaattac cagaagtctc
     3361 agttcaatcg cgtcttccag gccaagcgcc aaccgggctc gatcttcaag ccattcgtct
     3421 acctggcagc gctgatgtac ggagcggaga acggtaggaa gtttaccgca atgaccaagg
     3481 tagacgactc tccgtttacc tgggaatacg atcggcagga atggaagcct ggtaattaca
     3541 acgacgagtt tttcggtact gtgacgcttc ggcgggcgct tgaaaaatcg ctcaactcgg
     3601 caaccgctcg ggttgcccgc gaggtcggta tccggcgcat ccgtgacctg gcgcagcgtc
     3661 tcggcattca gagctcgttg cccgcggtgc cctcactagc attgggtgcg gcggaagtga
     3721 ccccgcttga agtagcggtg gctttctcca ctttagccaa caatggcgtt cgcacgcggc
     3781 cattaactgt taaacaggtt cttgccccgg acgcgcgtgt gttggaaaag cgcgatgttc
     3841 gggttgaaaa agtcgtttcg gcgcagatgg cgtttttaat caatcatctt cttaaaggcg
     3901 tgctcgatcg gggtacggcc gacagtgcgc gacgcgctgg attcacgcgg ccggccgccg
     3961 gcaagaccgg aacgaccaat gattacaagg atgcctggtt cgtcggatac actcccgatc
     4021 tcttaactgt ggtttgggtg gggttcgata accaaagcaa actcggtctc tccggttctc
     4081 aggcagcgct gccaatctgg accgatttca tgatccgcgc caccgccgga actcccatga
     4141 cggattttgt ggcgcctcca gggatcaagt ttgtcgatgt cgatccgatc agcggccaac
     4201 ttgccacggc aagctgtacc tccgtgatcc gtgaagcttt tatcgagggg gaagagccgg
     4261 cgctcacatg tgctttgcat ccgggagagc agatgcaagc tcctcggcta gaatgatgct
     4321 tgcttcaaat agattgcatc agccaggaaa gccgcgaccc caattggcaa tcgcgatggt
     4381 ccttggtttg tgcatcttgg ctgttcagag ctgcgcgccg caacggcctt atcgccgacc
     4441 tgtgccgact gccgcggcta aacctcagcc cgaaccagcg ccaattccca gtccgccgac
     4501 ttctgcgccc gtctcggagc tcagtaatcg gtcgatcccc cccgacccga aaatacgcga
     4561 gcaagatctc aaagcaaacg aaaaggtgct gcagccatcc ataacaactt cgaaggaagc
     4621 gccgccaccg attgcgccgc ctgaaactcc tccgccggcc aaaccgccgc tggtcgacga
     4681 cagctcactg ttagccaaga tcattcctgg aacaccgccg caacgagccg catctctgcg
     4741 cttgtccgaa gaagcgcgaa agctattgga aagcggcgat tacagtaggg cactcagccg
     4801 cctggagcgc gccattgcga ttgattccac caatgcctat ggtcattttt tgttggccaa
     4861 aactcagttc ggtctcaagc actaccaaga ctcgcttaat tttcttgatg ttgcggagtc
     4921 tcgtctcggc ggcgagccgt tttggttggc ggaagtctac gcgctgaaag gcgaaaacta
     4981 tcgtgccctt ggcatggctg aacgcgctga agaaagttat gctaaggctc tggcaatcaa
     5041 tcccgacaat cgcactgcga ccgaagccct gttccacaaa cagtcgggaa cccagccggc
     5101 ccagcgctaa tgattatgcc atgttagttc tcggcattga gacttcctgt gatgataccg
     5161 ctgccgcggt tctgctcgat ggccggacga ttttagcaaa tgtggtctcg tcacaggacg
     5221 aaattcacgg tgcttatggc ggcgtcgtgc cggagcttgc ctcgcgccaa cacatccgca
     5281 atatcctgcc gatcatcgat ggagctttgg gaaaagccgg cgttggtgtc agagatctcg
     5341 acggtatcgc ggtcacccat ggtcccggac ttgtcggctc gctcctggtc ggtttgtcgg
     5401 tggccaaagg catggccttt cgctggggca cgccctacgt cggagtgaat cacctcgaag
     5461 cgcacctcct ggcgattcat ctcgagcatg aagtgtcttt tccatacatc gctctgcttg
     5521 cttcgggagg acacacgctt ctctaccgtg tacagaacgt cggcgattat cactacttag
     5581 gcggcacccg tgatgacgct gccggcgaag cttacgacaa ggtggcaaaa atgatggggc
     5641 tgggctatcc gggcgggcgg gtaatcgata gtctggccaa aaccggtgac cgaaacgcaa
     5701 tcagatttcc acgcgcgcga ttgaaaaaga gcccgtacga tttcagcttt agcggtatca
     5761 aaacctcggt cttgcagtat ctcaaagcgc aagatcatca agcttggaag gcgcacgtgc
     5821 cggacatcgc ggcgagcttt caggaagcag tggtcgatat gttggtcagt ccgacggtta
     5881 gtgcggcgct cgctcaaaac atcaaccgca tcgtgctggc aggcggggtg gcggcgaata
     5941 gccggttacg ggaaaagata agtgagagag cggcgacaaa agacttgcaa gtttactttc
     6001 cttccgcgaa attttgcacc gacaatgctg cgatgatagc cctcaccgga caccactgga
     6061 tcaaaggagg ccgccgggac gacttcgctc ttaacgccca tgccgatcta accttgtagt
     6121 tggattgagc tgtcatgttt cgacgctgcg atcgataggc atcatgccga ccctgtacga
     6181 ggaagtgcgc gcggcattgc gtgaatccga gttcagaccg agcaaacggc tgggccaaaa
     6241 ctttttggtc catgagcatg tgctccaggc tattgttgat cttctcgcgc ttaaaacgga
     6301 cgatttagtg ctggagatcg ggccagggtt gggagccctc acccgtcgat tggttgaccg
     6361 agcgggcgca gtgtgggccg tcgaggtgga tccttttctg atccggcgat tgcaacaaag
     6421 cgatctattc gccgatccaa aatttcacct tatttctggg gacatcctca aagtgccgct
     6481 ggcaggaatc ctgcccaagg ataaaatcaa gctgatcggc aatctgccgt acagtatttc
     6541 cacgccggtg cttttcagag tacttgaatt gcgggattac ttttcttcga tcgtcttgat
     6601 ggtgcagaaa gaagtcgctg agcgaatcgc cagcgcccca gggacgaaag cctacgggac
     6661 attatccgtg tggtgccaga ttcacgggca ggtgatgcaa aaaatccagg tctcgccgga
     6721 agcgtttttc ccccggccga aggtgcgatc taccgtcctt aaaatggaac tttacccgcg
     6781 gccacgcgtg cctgcgcagg agatgcaggt gctgcgcggg ttgctgcgcg cggcttttgg
     6841 acaacggcgc aagactctcg gcaatgtcat aggcaagtgg ctcaagacgg gccgcgacga
     6901 cgttgaggaa tttcttggct cgcaaaatat cgatccgcag cgccgcggcg aaacgctaag
     6961 catcgacaac tttatagatc tgacgcacgc actggcctcg agccatctgc tgagagccct
     7021 gtcggcggat gcctcggctg atcatgatag gcctcagatt gattgctgaa gtgtatgcct
     7081 gaactgcccg aagtcgaaac tgtatgccgg agtttgcggc ctcatctgct ccatcggacc
     7141 atcgagcgag ttgaaacact ggagacgcgc ttgcgcgtgc ccgtagatga gctcgctctg
     7201 gcaggccttg ccgggaagaa gatatacgaa attcggcgag ccgctaaata cattctaatt
     7261 tcgttatccg acgaatcagt ttgggtgttt catctcggta tgtccggcaa gcttgtctgt
     7321 gtcggagccg atgcgccccg tcgaaaacac gatcacatca tcgtccattt agttggcggc
     7381 ggccagctgc gttatcatga tccgcgtcgt ttcggcctca ctctagtgac acgcaaagaa
     7441 atattgcacg aactgccgca attgcgtctt ctcggcattg atcccttcga tccagacttg
     7501 accggtgaat atctttttcg ccacacgcgc tcttccgacc ggcgcattcg ggacttattg
     7561 ctcgaccaac ggatcatcgg cgggctcggg aatatctatg ccaacgagat cttggccagt
     7621 gtcggcatca agccgacgat ccgcgcgtgt cggctgacgc ggaaacaggt atacgccatc
     7681 gcgcagggcg ttccccagtt gctgcagcaa gcgattcgtt ggtgcggcac atcgttttca
     7741 gattatcgca ctgccgacga cgaacgcggt gcgttccaat cccatcttcg cgtttacgat
     7801 cgggaaggac aaaaatgcag agcctgcgga tgtatcatca agcgggttgc catcggtaat
     7861 cgcagcgcct tttattgccc ttcctgccag aaatgatcga cgcaccatgg cgaagagacg
     7921 acatgccggc cgcgatcctg atctttgcgg attcggaaga ctaccagtcc ggcaaactct
     7981 ccgtctctcc gacgacaatc acgatatgga cttaaccgat ggcgtcgaaa aattgaatta
     8041 ttctatcgag gggggcaact ccagaggcgg agtatgcttc cctgtaccaa tatcgctaag
     8101 ccttatccgg cgcgccgttg ttgcctccct tttgcttgat gacattcatc gccaacgaaa
     8161 tcatcgagcc cacgtcgctg agcgtcgcgg gaagaatcat cgtattgcct tcctgtgcga
     8221 gcttgccaaa ttgggtcagg tagttatcgg cgacacggag ttgtaccgct tcgaacccgc
     8281 caggctgttg aatcgattcc gcgaccttgc gaataccatc ggcggtcgct tcggcaatcg
     8341 ccaggatggc tgcggcctcg ccttcggctt cgttgatttg ctgctgcttg ttggcttcgg
     8401 aagctttgat gacctgttgt ttttcgcctt cggcgctgtt gatcgccgca tctctttgtc
     8461 cttcggaggt tagaatgagc gcgcgctttt cgcg
//
LOCUS       DASUVS010000089         2378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_904206, whole genome shotgun sequence.
ACCESSION   DASUVS010000089 DASUVS010000000
VERSION     DASUVS010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904206"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(397..1062)
                     /locus_tag="VF182_05005"
     CDS             complement(397..1062)
                     /locus_tag="VF182_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009488891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HEX6436467.1"
                     /translation="MKEKLRPHGLSLALFGFFIIFQLGLSGVGQRHYNHEQIQHGQPT
                     VGYLEYIASAGFLEATMENWESEFLQMFAYVLMTAFLFQKGSAESKNPDQPEAVDRDP
                     RESRNKKDAPWPVRRGGVVLKLYENSLALTLFLLFLISFMLHAIGGASEYSDQQIAHG
                     GQAVTVFQYLGTAQFWFESLQNWQSEFFSIAILVVLSIFLRQKGSPESKPVDSPHSMT
                     GHE"
     gene            1620..1838
                     /locus_tag="VF182_05010"
     CDS             1620..1838
                     /locus_tag="VF182_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436468.1"
                     /translation="MSESRETVRAYTREYIRLRKETNRLLREGKIDKNEANHQNGNAK
                     QFLMFARHQLEAAKKAENLKSSASLSHN"
     gene            complement(1894..2235)
                     /locus_tag="VF182_05015"
     CDS             complement(1894..2235)
                     /locus_tag="VF182_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6436469.1"
                     /translation="MARLTLWPRPKRSVNRFLRLGLSHNIIKVIRWNARWLVLCDIAL
                     PWMDGYAIARKIRQDPELKDVHLIAISGYGQDEDQQLARDAAFNEYCVKSEDLRSLDQ
                     LIKNRQGPSRR"
BASE COUNT          584 a          626 c          633 g          535 t
ORIGIN      
        1 cgtcagaatg cgcggaagtc gatgtctcga tctaaatcgg cagatcgagc aacacacgga
       61 gaatcggggt catcaagttt agatccctga cggctctacc ctcgctcatt tcagcagacg
      121 actccttctc gcttccgaac acagcgcgcc gctcggtagg atccagcggc tccctcgatg
      181 tcgaccgcgc cgcagttatc caaaatactc gcaatccgct cggcgatggc atcattctcc
      241 gccgcgagat ttgtttcaac cgagctagga ctggatcgat cgaaggtgtt gattgtgaat
      301 cgtactgccg ccgcgccgct cccccggcaa tgtagatggt tgcgctcttc aaagagagag
      361 gcgcagaagc aatactggct gattgcttaa cggatcttat tcgtgccctg tcatactgtg
      421 cgggctatcg acaggcttgg attcaggtga tcccttctgg cgcaggaaga tgctcagcac
      481 aacaagaatc gcgatcgaaa aaaattcact ttgccagttc tgcaacgact cgaaccagaa
      541 ctgcgcggtg cccaaatact gaaagacagt gacggcttgg ccgccatggg cgatttgctg
      601 atcgctgtac tcacttgcac cgccaatggc atgcaacata aaggagataa gaaacagaag
      661 aaagagcgtc aaggcgagtg agttttcata aagcttgagg acgacaccgc cccggcgcac
      721 cggccacggc gcatccttct tgttgcgtga ttcccgggga tcacgatcca ccgcctcagg
      781 ttggtcaggg ttcttcgatt ccgctgagcc tttctggaac agaaaagccg tcattaggac
      841 gtatgcaaac atttgcagaa actcgctttc ccagttttcc atcgtggctt cgaggaaacc
      901 ggcgctagcg atgtattcca ggtaaccgac tgtcggctgg ccgtgttgga tctgctcgtg
      961 attgtagtga cgctggccca ccccgctcag ccccaattgg aagataatga agaacccaaa
     1021 cagcgccagc gaaaggccat gcggccggag tttttccttc atagtatcag cacctctttt
     1081 caccaatgga aatccgacga gcgccggctg ttcgcgggtg ttctcgccgg catccctgat
     1141 ggcgttatca gagcaggtta tcgcaaccct tcaaatctac aatcccgaat gttagctgtt
     1201 cgaaatacga atgatacgtt caagcctggt aaggcatgca atggtatatg ctcacagcca
     1261 tttgcaatgt taggtcttat tgtctgctca caagatcgtt cgtataatcg agcctgttgt
     1321 aatcggaagg gggcggaaaa atagcatcct atgaggcagt ttttgcgtag taagctccgc
     1381 ccatcgatga ccccgagttg ctattgcagg acggctttta ccgccgagat ttcttattga
     1441 ctcgcaccat catcggagct atgaagtggg ctgactcagt ccggttcaat tcagaaatag
     1501 ttcaaggacg tcgattaggc gaaggggatt tgccaggcag ttcggcgcgc gtcttgctgc
     1561 cgtggcatag cggtgccgct aaattccgag ccgagtaagt gcacgtgaaa ggatgggaaa
     1621 tgagcgagtc gagagaaact gtcagggcct acacgagaga atacatcagg cttagaaagg
     1681 aaaccaaccg gcttcttaga gaaggcaaaa tcgacaagaa cgaggccaat caccaaaatg
     1741 gcaacgccaa gcagtttttg atgttcgcac gccatcaact cgaagcagcg aaaaaagcgg
     1801 aaaacctaaa aagctccgca agcttgtcgc ataactgaaa gcgagagtca gaagacaccg
     1861 gggattcaaa ggagcagtga acttcaaatg cgcctagcgc cggctgggac cttggcgatt
     1921 ctttatcagc tggtcgaggg atcttagatc ctccgacttg acgcagtact cgttaaatgc
     1981 ggcgtctctg gctagttgct ggtcttcatc ctgtccgtat cccgagatcg caatcagatg
     2041 gacatctttg agctccggat cttgccggat cttccgtgct atcgcgtagc catccatcca
     2101 aggtagcgcg atgtcacaaa ggacgagcca ccgtgcattc caacgaatca ctttgataat
     2161 gttgtgggaa agcccgagtc gcagaaaccg attgacggat ctcttcggcc tcggccataa
     2221 cgtgagccta gccatcgatc aaggaccgct gttctcaagg ttcgtgatac ggggaccggc
     2281 atgggtgaga gcacgttgac ccgtttgtgg cattcagcca ggcctctcga actttcgcat
     2341 cgttgacgag gcaggctcgg ctcaggtctg acattaat
//
LOCUS       DASUVS010000090        16592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1342161, whole genome shotgun sequence.
ACCESSION   DASUVS010000090 DASUVS010000000
VERSION     DASUVS010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 16592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16592
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342161"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(521..2092)
                     /gene="ggt"
                     /locus_tag="VF182_05020"
     CDS             complement(521..2092)
                     /gene="ggt"
                     /locus_tag="VF182_05020"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX6436470.1"
                     /translation="MTREFSTWAKNGMVASSQPLATLAGVQILINGGNAIDAAIATAA
                     VLGVVEPSSIGIGGDAFCLFYSAKEKKIKALDASGRSPRAASLEFCRKSGFQEMPQRG
                     IHSVTVPGAVHGWATLLDAYGTTKLADVLQAAIGHAEHGFPVAELTAEQWKESQARLA
                     ADDGGSLNYLTEGRTPRAGEIFKNPRMAKTLRRIADGPEVFYRGDIAEKIVRCSEKNG
                     GLFTLEDFAGHRSDWVEPITANYRGYDIYELPPATQGFVALEMLKILEGFDLKSLGGN
                     SADALHLMIEAKKLAFADRDRHLADRDFMKVPATDLLSRNHADLQRQKIRADHAAGEM
                     INAPSGTDTEYVAAADREGNLVSFIQSNFMGFGSGVVEPETAIILQNRGHLFSLDENH
                     PNCIGPHKRCVHTLMPGMILRHGKPYAALGLKGGHVQPQAQAQIISNLIDFDMTVQQA
                     ISAARFNHLDAEKVALEPEFPQEVRDELSRRGHWIAGGTPESFGGAHAIVIDPQSGAF
                     VGGSDPRKGGCALGY"
     gene            complement(2138..3157)
                     /locus_tag="VF182_05025"
     CDS             complement(2138..3157)
                     /locus_tag="VF182_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6436471.1"
                     /translation="MRAIGCKLFLLAWFVALSVIAAGAQEGSDPKIIQAARNEGEIVW
                     YTTMSADQSTAFMERFRRKYPFLKPSVVRLGGNALLSRIVTESKAGKSYFDVSHGTGE
                     IVLPLMNMGLLASYASPERKMIPDDLKDKNGFWTSVYVNSIVLGYNTQLTKGQPVPRT
                     YDDLLQPRWRGQKISVDSTYVTLLQGLSSAWGKDKAVDYLKKLGEQEPVVMRGSTVRA
                     QLAAAGEFPLVIAYANIIQYLTEKGAPVDWVALEPAVISVNTVMAGAKASHPNAAKLF
                     IDFTLSKEGQEKLWDFQRIPSRSDVEPKPARLFRGYKRHVVHPEEMQNLDETAKLYSQ
                     ILKTR"
     gene            complement(3240..3593)
                     /locus_tag="VF182_05030"
     CDS             complement(3240..3593)
                     /locus_tag="VF182_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6436472.1"
                     /translation="MRPKVRHIAVMTKNREKMVEFYQKIFGLEPKRGFGGAIYMSDGD
                     VNIALIEVKRDDQKEGINHFGFEVDDLDDIKRKLREAGFPAEIDTKSDKDSDHRIQDP
                     DGHWVDMAVKLRWPK"
     gene            complement(3614..4753)
                     /locus_tag="VF182_05035"
     CDS             complement(3614..4753)
                     /locus_tag="VF182_05035"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6436473.1"
                     /translation="MQLKHGFISADDHVQEHPEVWSSRMSKKKWGERIPHVEKLHDGS
                     ESWVIDGRRLLLPGIALAGALMADRAREPQRWSEVPPAAFTAADRLKAMDCDGIDCSV
                     LYPIVGGLAGESFGKLDDPDLELACVQAYNDWLIEEWASVSPRFVPQCIVPIWPMERT
                     VMEIKRAVAKGHRGVIYPASPMELRAVPHINEPAYDPLWAVCEELGVPISFHSGASPR
                     IQMRPGDDFSPALAAAFSNVIRPVSSIAVVANFMISRVLHRFPRLKIVFAEGSLGWGA
                     YEMEYADYQSNADGLPSEGYALKPSELFRRQCYFTCWYDRMSLRVRDYLGTANILWSS
                     NFPLATSTWPNTRHRAELAFAELPEIEKRQIQWQNAAKLYSLESA"
     gene            complement(4943..6085)
                     /locus_tag="VF182_05040"
     CDS             complement(4943..6085)
                     /locus_tag="VF182_05040"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6436474.1"
                     /translation="MEIKYGLISSDSHGQLGKDAYTSRMSVSRWGERVPHIVEVRDEK
                     FDAPVERWAVNGEIQGGNVCNCPTAMERRGYYPQRWEEVPLEVYDPASRLRALDRDGV
                     DAEVLFPNGPGGTFLYGDPEFELACVRAYNDALAEFRRASQRFIPLAAIPFMSPIETI
                     VAEVERAVEAGHLGINMLADPSVAVKSQKRLADPFWGPLWDACQQLGIAVHLHASAGL
                     AGKLSVPQWKGYTGRQAHCVSTLRNFCTATQIVPYLIFSGTLEKFPRLNWVFAETGLG
                     WVSSVLEACDYEWEQRHLWTEGILTRPSELFRRQIYVDFWFETVGIQMRDYIGIDNIL
                     WESDFPHITSTYPKSWAAVEHSLAGVAAPDREKVLFRNAARLYRLT"
     gene            complement(6140..6838)
                     /locus_tag="VF182_05045"
     CDS             complement(6140..6838)
                     /locus_tag="VF182_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436475.1"
                     /translation="MSLWPKTRQELDALVNRHFETPEYQDFFSVKLTPERQKVIDLHY
                     PHYIKSRRDCWGAAAVRAPLDVKRAIWEHEKDELIFDKRLGAAHLTDEQMAEATDETN
                     LLPAVRAVFLAWMHLATTRPWIESLMVNHITERKNNPAIVKGGGFTARYAHKKVADLG
                     GTLESLDDNTKVHMVADEDHSDMFEPIFDKYVTDERIAQAVVRAAHDSLDVDRAYRSA
                     LATAMMRIAGSRAA"
     gene            complement(6916..7950)
                     /locus_tag="VF182_05050"
     CDS             complement(6916..7950)
                     /locus_tag="VF182_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6436476.1"
                     /translation="MKADFIQIQRIWPALMLIWLILWLAPHAGAAGDPKVIAAAKKEG
                     QLFWWSTIAQDQSQKIIDEFMKLYPFIKANYWRSGSVGLHNKILLEARAGRSSWDVVS
                     QTTPEFIYELKQKKLIAPYESSERRHFSQDLKDKEGFWTGTYALPTGVGFNTQQVKRE
                     DAPRTYQDLLNPNWKGRKISVDDEGYELLVGLGEAWGASAATEYLKRLAAQDPIVGRG
                     NSQRAQLLAVGEFPLAIAYTHTVEWSRSQGTPVDWVNLEPVVIKFDGIMLGSKAAHPN
                     AAKLFIDFILSQSGQELLQSFSRVTLRDGVDPNPPRLIKGFKRIVLHPEKGQDAQQSL
                     KTYRQIFGLP"
     gene            complement(7947..8657)
                     /locus_tag="VF182_05055"
     CDS             complement(7947..8657)
                     /locus_tag="VF182_05055"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6436477.1"
                     /translation="MNESQWHQTMGEMVRDYIRSPELEHYFSIKMTKARAAVMVTQQS
                     LYVRHRRECWAHISANCPILGVKQRILEHEYEEIIRDDFSDYGHLHLIINQGKSVGLE
                     PKQVLNAEPLLETRAVLYAWSWITSELSWIEGLAAMTITEWCNDDRLLGDLGGGQSTR
                     MAKKWMEEMGFTWKQIPNLQAHSQADEKHSDMFLPFLSEYATSGKEQLALTAAKRSLD
                     FNALFRKGIADAQEKISV"
     gene            complement(8654..9715)
                     /locus_tag="VF182_05060"
     CDS             complement(8654..9715)
                     /locus_tag="VF182_05060"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436478.1"
                     /translation="MNKSIKFYGPHAPLKRLPGDFKPPKKVKGSILRFVVFAKLILTL
                     VLISSQPASSADAGKEKVAFAYAAISPSMSGIWMAKEIGAFDRNGLQAELVHISSGAT
                     AIQALVGGSVHAALGASNAVVAAIIKGAPIIAVGSNTSRPGMQLWVQPDIQRAEQLQG
                     KTLAITRFGSTSDFVTRLIMRKLNLEGKAELRQFGGVVEADVGFRAGQAEGRVSSQAP
                     GPKAKMLVDAAELGIPFSMNLLAVSNDFYRRSPKTVERIVSSYIEGIAALKTRKAQGL
                     KVLEKYMGQRGGSAEQHYEFVTKYLDAIPRVDPAAIETVLEMVGQKSPPKVQLFDNAI
                     VDRLAQEGFIDRLYKGGKS"
     gene            complement(9746..10456)
                     /locus_tag="VF182_05065"
     CDS             complement(9746..10456)
                     /locus_tag="VF182_05065"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6436479.1"
                     /translation="MAANGWHEAFGELARATMSSPELERYYSIPISRPRAQLILKQLS
                     LYVRHRRDCWAYVSGNCPVLAVKQKILAHEYEEMIRDAHSEHGHLDLIIRQGKPIGLT
                     AEDILQTKPIPTTAAALYAWGWMCKKKSWLEGLAAMTMTEWNQDDRLLADLGGGHSAR
                     MGNLWVKYLGITWDDLPNWKAHREADKGHVDMFLPIWEEFGSGENREMILQAAKESEE
                     LYRLYQKGLADAMQALPD"
     gene            complement(10533..11240)
                     /locus_tag="VF182_05070"
     CDS             complement(10533..11240)
                     /locus_tag="VF182_05070"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6436480.1"
                     /translation="MKRAEWRRQLGELVRDAMRAPELDHYFRVKITKPGAQILITQLG
                     LFIRHRRDCWAHVSGNCPQMSVKQKILQHEYGEVIKDEYTEHGHLDLIVRQGKALGLS
                     PQEVLEVEPLPTTFATLYAWAWMTREKSWIEGLTALTITEWANDDRLLQDFGGGLSSR
                     MAKRWMDDLGFGWEQMPNFKVHSEADEEHSDMFLEDLEKFAIGEKEALAYRAAKESME
                     LLKIFRAGIAQDMAKCV"
     gene            complement(11253..12218)
                     /locus_tag="VF182_05075"
     CDS             complement(11253..12218)
                     /locus_tag="VF182_05075"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436481.1"
                     /translation="MSIVRCLVRFDAALIVFFLVLASGLSTAQTPPKVTIAYAAISPI
                     FAGVWMAKDMGAFEKHGVKADLIYISSGSVTVQAMVGGNLDMSVAASNAVVSSILRGA
                     PLVAVGSITNRPAMSLFVQPEITRPEQLQGKVLGITRHGSSTHFLTQFVIEKLGLQDK
                     VKIQPMGGTREVDTAIRTGMIAGAVRSMKPGPKVRMLVDLPDMGIPFSMDLIAVKRDF
                     YKSSPRIVEAMLKAYIEGVAGLRSRKEFAYKVLAKYLLGGGESLDEAYAYAVKYLDRN
                     ARTDPAVIQTVLDWEGKKDSSPNEFFDNSIIDAIIKEGFVDRLYK"
     gene            complement(12315..12653)
                     /locus_tag="VF182_05080"
     CDS             complement(12315..12653)
                     /locus_tag="VF182_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6436482.1"
                     /translation="MEKPRIRHIAINVEDLDKTAEYYKKVFGMEEKHRRPGGTIYLSD
                     GHLDLALIHSEKYPWGIHHFGFKVESVKEIEKIADTKAEDNTYGAIAESWIKDVEGNR
                     VDVSEHGWPV"
     gene            complement(12682..13395)
                     /locus_tag="VF182_05085"
     CDS             complement(12682..13395)
                     /locus_tag="VF182_05085"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6436483.1"
                     /translation="MQVEQWRNAIGEMVRDHMRSPELEYYFSVKMNKQRAQLMITQLG
                     LYIRHRRDCWSLVSANCPVMAVKQAILRHEFGEVIKDQYSEFGHLHLIIRQAEKLGLS
                     AREVVDTKPLLSTTATLYAWAWLTHAKSWVEGLSALTVTEWTNDDRLLNDIGGGHSTR
                     MGRRWMDDLGLAWRDMPNFVAHSQADEEHSDMFLPFLAEYATGEKEELALAAVKESLA
                     LFALYREGVARAMEKIPLS"
     gene            complement(13480..14460)
                     /locus_tag="VF182_05090"
     CDS             complement(13480..14460)
                     /locus_tag="VF182_05090"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436484.1"
                     /translation="MQLLRDVLRSALMITVCALLFKPAELAAQTKKIVFGYSTVGAMA
                     AGSWMAKEIGAYEKYGIDAEMIYISSGPTTVQALLGGDIVGGIAATNAVIAAVLRGAP
                     LVSVLSTANRPYHRFWVQPEITRMEDLKGKTIGVTRFGSVTDNLTRMLLKKYGLENAV
                     SVRQMGGTIEVSAAFQNKQIAGAVTSSLRVDAGMQPRLLLKLEDLGFQYSMDVVAHSR
                     DDLKRFPHVVDGMVRAYTEGVAAIHSQKERAYKAIAKYARLRDQKKIDEIYQDSIVYL
                     EKTPRVEPEAIDSILEFMGKKGIPLETFADNSMVDKMVREGFVDRLYKKP"
     gene            complement(14650..15384)
                     /locus_tag="VF182_05095"
     CDS             complement(14650..15384)
                     /locus_tag="VF182_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6436485.1"
                     /translation="MMTIKTRRLDQEKDGIEGYIAHPEQTQKGPALLLIHQHSGLTGY
                     LKTAAYRFAQLGYTTVVPNLYHMLGYPAETHIDKGTEIQNKTADPDFVRVIDQGWRYC
                     LSRNDVDRSRVGVIGYCMGGRIGIHFVAATPAVRAFVAYYPSVREEPPTPIRPRHPYD
                     AAREIKCPSMVLYGGNDRTSTFAIQQKVMESFHANGQPLEWHYFHFGNHGFASTESDG
                     YQPYLADLAWPLVTEFLGRELISPVL"
     gene            complement(15403..16383)
                     /locus_tag="VF182_05100"
     CDS             complement(15403..16383)
                     /locus_tag="VF182_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436486.1"
                     /translation="MSLGSKLCKTAVFAVSIFLGVHIHSAAEAQSRVKFAYSSVGSMA
                     TGVWMAKEIGAFEKNGIQADIIFISSGPVVVQALLGGDLLGGSAASNAVINAILNGAP
                     IVAVGGTANRPYHRLFVQPDINRLEDLKGKTLGVTRFGSITDNLTRILLRKHGLEGNV
                     NVRQMGGTMEVAAAFQNRQIAGAVTGELRGPSLTVPKILVNLVDMGVPYSMNMLAVSR
                     DTLKGSPETVEGIVRAYAEGVAFMNQNKERSLKIIAKYGHLTDPKQIEDHYRDSVTYL
                     DRIPLAQPEAVQTILEFMGKRGIPPETFQDNSIVEKLSREGFFEKLYKKS"
BASE COUNT         3538 a         4841 c         4339 g         3874 t
ORIGIN      
        1 cttaacaaag aagttgtatg cgagatatgc atcatctggc tttgcgacaa actgatcgaa
       61 ctcccattcc agcttcttga aaagatcagt gtgactactg agaccgaaga cgcctttcat
      121 acctatctca tcaaggttcg atccacataa cgttttgctc tgcggcggcc gccacacctg
      181 actgattggc actcccgttt cggcccgtcc gcgagcgccg ttcgtcttca aagaggccgc
      241 gggacggcca gcggaccagc gcataagcta gatgtctttg ttgatctgct cgtcgatttt
      301 tacgagcagc cctgctttat cgagcgctac aacgccgtct cgcagagccc tatagtagct
      361 catcaatact cttcccttga gcagtgtcct tgcgcttggg gatcatcatt agatttctca
      421 tgacaccacg aaacagccgt gccagcctat agcttaaacg ccgcaaaggc gcgctcggcc
      481 taaacgtttc gaacggattg aaggacttaa acgcggatca tcaatatccc agcgcgcagc
      541 cgcctttgcg cgggtccgac cctccaacga acgctccgga ttgtggatcg atgacaattg
      601 cgtgggcgcc gccgaagctc tccggagttc cgccggcgat ccagtggccg cggcgcgaca
      661 actcgtccct gacctcttga ggaaattccg gctccaaggc tactttctcc gcatcgagat
      721 gattgaagcg cgccgccgag atcgcttgct gaacagtcat gtcgaaatcg atcagattag
      781 aaatgatctg cgcctgcgct tgcggctgca cgtggccgcc tttcagtccc agcgcggcgt
      841 agggttttcc gtggcgcaaa atcatgccgg gcatcaatgt atgcacgcag cgcttgtgcg
      901 ggccgatgca attcggatgg ttctcgtcta gtgaaaataa atgaccgcgg ttctgcaaga
      961 taatcgcagt ttcaggttcg acgacgccgg agccgaagcc cataaaattg ctctgaataa
     1021 aggacaccaa attgccttcc ctgtcggcgg cggcaacata ttctgtgtcg gtgcccgatg
     1081 gcgcattgat catttcgccg gcggcgtgat cggctcgaat tttttgccgc tgcaaatcgg
     1141 cgtgatttct ggataggaga tccgtggccg gcactttcat gaaatcgcga tcggcgaggt
     1201 gtcgatcgcg gtcggcgaaa gcgagcttct tcgcctcgat catcagatgc aaggcatcgg
     1261 cgctgttccc gcccaatgat ttcagatcga atccttcaag gatcttcagc atctcgagcg
     1321 cgacaaagcc ttgagttgcc ggagggagct catagatatc atagccacgg taattggccg
     1381 tgatcggctc cacccaatcc gaacgatgac cagcgaaatc ttcgagcgtg aacagcccgc
     1441 cgttcttctc agagcagcga acgatcttct cggcaatatc gccgcgatag aaaacctccg
     1501 ggccatcagc gatccgccgc aacgtctttg ccatgcgcgg gttcttgaaa atctctcctg
     1561 ctcgaggcgt ccggccttcg gtgagataat taagtgatcc gccgtcatcg gctgccagtc
     1621 gcgcttggga ttccttccac tgctccgccg tcagctcggc cacaggaaaa ccgtgttcgg
     1681 cgtggccaat cgcggcttgc aggacatcgg cgagcttggt cgtgccgtag gcatcgagca
     1741 gcgtcgccca gccatgcacc gctcccggaa cggtgacgga atgaatgccg cgttgcggca
     1801 tctcttggaa gccgctttta cggcaaaact cgagactcgc cgcgcgcggc gatctgccgc
     1861 tggcgtcgag ggctttgatt ttcttttctt tggctgaata gaataaacaa aatgcatcgc
     1921 cgccgatgcc gatggagctc ggctcgacga ccccgagcac cgcggctgtc gcgatcgccg
     1981 cgtcgatggc attgccgccg ttgataagaa tctgcacacc ggcgagggtc gccagcggct
     2041 ggctcgatgc aaccatgccg ttctttgccc acgtggaaaa ttctcttgtc atggattctg
     2101 gttccgccag cgcggcgcat gccgcgctga ttgcgcctta gcgcgtcttt agaatttgcg
     2161 aataaagctt cgccgtctca tcgaggttct gcatttcctc cggatgcacc acgtgacgct
     2221 tgtagccgcg aaacagtcgg gccggctttg gttcgacatc gctgcgcgag gggatgcgtt
     2281 ggaagtccca aagtttttcc tgcccttctt ttgacaaagt gaaatcgata aacagcttag
     2341 ccgcgttggg gtggctcgcc ttcgctccgg ccatgacggt attgacggaa attaccgcgg
     2401 gctcgagcgc cacccaatcg acgggcgcgc ctttctcggt gagatattgg ataatgttgg
     2461 cgtaagcgat caccagagga aattctccgg cggcggccaa ctgagctcgc acggtgctgc
     2521 cgcgcatgac caccggttct tgttccccca gctttttcag atagtcgacg gccttgtctt
     2581 tgccccaagc cgaactcaag ccttggagaa gggtcacgta ggtcgaatcc acggaaattt
     2641 tttgcccgcg ccatctcggc tgcagaagat cgtcgtaggt gcgtggaacc ggttgtccct
     2701 tggtgagctg ggtgttgtaa cccaacacta tcgaattgac atagaccgat gtccaaaaac
     2761 cgttcttatc cttcaagtcg tccggaatca ttttacgctc cggagaagca taggaagcaa
     2821 gaagccccat gttcataagc ggcaggacaa tctcgccggt gccatgggac acatcgaaat
     2881 agctcttgcc ggccttggac tcggtgacga ttcgacttag caaagcgtta ccacccagtc
     2941 gaacaacgga aggcttcaaa aatgggtatt ttcggcggaa ccgttccatg aacgcagtgc
     3001 tttgatccgc gctcatagta gtataccaga cgatttctcc ttcgttgcgc gccgcttgaa
     3061 tgatcttggg atcggatcct tcctgcgcac ccgcggcaat caccgataag gcgacaaacc
     3121 acgccaacag gaagagtttg cacccaatcg ccctcatcga attttgcccc gaatcgattg
     3181 catggccgtt aatgccttca gcgccgcgcg tgcgaattag gtttaatacg tcgcggcttt
     3241 cacttgggcc agcggagttt gaccgccata tcgacccaat gtccatcagg atcctggatc
     3301 cggtgatcgg agtctttatc cgacttggtg tcgatctcgg cgggaaaccc cgcctccctc
     3361 agcttgcgtt tgatatcgtc gagatcatcg acctcgaaac caaaatggtt aatcccctct
     3421 ttttggtcgt cacgcttcac ttctataagc gcgatgttga cgtcgccgtc cgacatatag
     3481 atggccccgc cgaaaccgcg ctttggttcc agaccaaaaa tcttttgata gaactccacc
     3541 atcttttccc gattctttgt catgactgct atgtgtctca ccttggggcg catgttgttt
     3601 gcctcctcta tttttacgca gactcgagtg agtagagttt tgctgcgttc tgccactgta
     3661 tctgtcgttt ctctatctca ggtaattcgg cgaaagctaa ctccgctcgg tggcgcgtgt
     3721 tgggccatgt ggaagttgcc aggggaaaat tgctcgacca taaaatattc gcggtgccga
     3781 gataatctcg aacacgcagg ctcattcgat cataccagca ggtaaaatag cattgacgcc
     3841 gaaacagctc cgaaggcttg agcgcataac cttccgacgg caagccgtcg gcattggatt
     3901 ggtagtcggc gtattccatt tcatacgctc cccagcccag cgagccttca gcaaaaacga
     3961 tcttcagcct gggaaatcgg tgcagcacgc gggagatcat aaagttagcg accaccgcga
     4021 tcgagctcac cgggcgaatc acgttgctga aagccgccgc cagcgccggc gagaaatcat
     4081 ccccggggcg catttgaatt ctcggcgacg ctcctgaatg aaagctgatc ggcaccccga
     4141 gttcctcgca caccgcccag agcggatcgt atgccggctc gttgatatgc ggcacggctc
     4201 taagctccat cggagatgcc ggatagatca cgcctcggtg tcccttggcc acagctcgtt
     4261 tgatctccat caccgtccgc tccatcggcc aaatcggcac gatgcactgc ggcacgaagc
     4321 gcgggctcac gctcgcccat tcctcgatta gccaatcgtt gtaggcctgc acgcaagcca
     4381 gttccagatc aggatcatcg agcttgccga agctctcgcc cgccaaaccg ccgacaatcg
     4441 gataaagcac cgagcagtcg atgccgtcgc aatccattgc tttgagtcga tccgcggctg
     4501 taaaggccgc cggcggaacc tcgctccagc gctgcggttc gcgcgcccgg tcggccatca
     4561 gcgcgccggc tagcgcgata ccgggcagca aaaggcggcg gccgtcaatg acccagctct
     4621 cactgccgtc gtgcagcttt tcgacgtgag gaattcgctc accccatttt ttcttggaca
     4681 tacgggagct ccaaacttca ggatgctcct ggacgtggtc atcggcgctg ataaaaccgt
     4741 gcttcagttg catgacctct cttttttgct tacccaacaa ccagcaaatt tttgaaaact
     4801 ctgatcgcag gctcctcaaa aggactcaga ggcaaggcgc gcgaattaca gggatgagag
     4861 cggaaacctg aaagcggaat tcagttttca gctttcataa ttcatctttt ctaaaagcat
     4921 atgagtattt ttcaggagcc tgttaagtca atcgataaag ccgcgccgca ttgcgaaaca
     4981 aaactttttc gcggtcgggc gcggcaacgc cggctagaga gtgttccacc gcggcccacg
     5041 actttggata cgtcgacgtg atgtggggaa aatccgattc ccagagaatg ttgtcgatac
     5101 cgatataatc gcgcatctgt atgccgaccg tttcaaacca gaaatcgaca taaatctgcc
     5161 ggcggaagag ttcgctggga cgagtgagaa ttccttccgt ccagagatgg cgctgctccc
     5221 actcgtagtc gcaagcttcc agcaccgagc tcacccagcc cagtccggtt tcggcaaaca
     5281 cccagttgag tctgggaaat ttttccaaag tgccggagaa aatcagatac ggcacaatct
     5341 gtgtcgcggt gcagaagttg cgcaatgtcg atacgcagtg ggcctgcctg ccggtatagc
     5401 ctttccattg cggcacggac agtttaccgg caagacctgc cgatgcgtgg agatgcaccg
     5461 cgataccgag ttgctggcag gcatcccaaa ggggacccca gaatggatcg gctaaccttt
     5521 tctggctttt tacggcaacg ctgggatctg ccaacatgtt gatacccaag tggcccgcct
     5581 caaccgcgcg ctcgacttcg gcgacgatgg tctcaatcgg gctcataaag ggaatggcgg
     5641 caagcggaat aaagcgctgg ctggcgcggc gaaattcagc gagcgcgtcg ttataggccc
     5701 gcacgcacgc gagctcgaat tctggatcac cgtaaaggaa cgttccccct gggccgttgg
     5761 gaaacagcac ctcggcatcg acgccatcgc gatcaagcgc tctcaagcgg cttgccggat
     5821 cgtaaacttc caacggcacc tcttcccacc gttgcggata atacccacgc ctttccatgg
     5881 cggtgggaca attgcaaaca ttaccgccct gaatctcacc gttgactgcc cagcgttcca
     5941 ccggcgcgtc gaatttctcg tcgcgcacct cgacgatatg aggaacccgc tccccccacc
     6001 tgctaaccga catcctgctc gtgtaagcat ccttaccaag ctggccatgg gaatccgagc
     6061 tgatcaggcc gtactttatt tccatgctaa tccttcgatc agaattccga atagcgaatt
     6121 atcatagccg gggccaaact cacgccgctc tggagccggc gattctcatc atcgcagtcg
     6181 ctagcgcgct ccggtaggcg cggtcgacat cgagactgtc atgagcggct cgaaccaccg
     6241 cctgggcgat cctctcatcg gttacatatt tgtcgaaaat gggttcaaac atgtcggaat
     6301 gatcttcatc ggccaccatg tgaacttttg tgttgtcgtc gagactttcc aaagtgccgc
     6361 caagatcggc cactttcttg tgagcgtacc tggcggtgaa acctccgcct ttgacgatgg
     6421 cgggattgtt tttcctctcg gtgatgtggt tcaccatcag gctttcgatc cacgggcggg
     6481 tggtggccag atgcatccat gcgagaaata ccgcgcgcac cgccggcaac agattcgtct
     6541 cgtcggtagc ttcggccatt tgctcgtcgg tcaaatgcgc ggctcccaac cttttgtcaa
     6601 atatcagctc gtccttctcg tgctcccaga ttgcgcgctt gacgtcgagc ggagctcgaa
     6661 ccgcggcggc gccccagcaa tcacgcctgc tcttgatata gtggggataa tgcaggtcga
     6721 ttactttttg ccgctccgga gtgagtttta ctgaaaaaaa atcctggtac tccggtgttt
     6781 cgaaatgacg attcaccaga gcatccaatt cttgtctcgt cttgggccat aaactcataa
     6841 atacctcctc agcttgtttg tcagattaat catgtgctcg agcatcgatc aaccgttttg
     6901 ctcatgccgt acaactcacg gaaggccaaa gatctgccga taagttttca ggctttgctg
     6961 agcgtcttgg cccttttccg gatgaagtac gatgcgcttg aagcctttga tcagccgcgg
     7021 cgggttcgga tcgacaccgt cgcgcagggt cacgcggctg aagctctgca gcagctcctg
     7081 ccccgactgt gaaaggatga aatcgatgaa aagcttggcg gcattcggat gcgccgcctt
     7141 ggagcccagc atgataccgt cgaacttgat aaccaccggt tccaggttga cccaatcgac
     7201 cggcgtgcct tgcgatcgtg accattcaac ggtgtgggta taggcaatcg cgagagggaa
     7261 ctctccgacc gctaaaagct gcgcccgctg gctattgccg cgtccgacga tcggatcttg
     7321 cgcggccagt cttttgagat actccgttgc cgcgctggct ccccatgctt ctcctagtcc
     7381 gactagaagt tcataacctt cgtcatcgac agaaattttt cggcccttcc agttgggatt
     7441 gagcaaatcc tggtaggttc tgggcgcatc ttcgcgtttt acctgctgcg tgttaaagcc
     7501 gacgcccgtc ggcagcgcat acgtgccggt ccaaaacccc tctttgtctt tgagatcctg
     7561 actaaagtgg cggcgctcgg acgactcgta cggcgcgatc aatttcttct gcttgagctc
     7621 gtagatgaac tccggcgtcg tttgcgaaac aacatcccaa ctcgaccggc cagcgcgcgc
     7681 ttccagaagt attttattat gcagcccgac gctgccgctg cgccagtagt tggctttgat
     7741 gaaagggtaa agcttcatga actcgtcgat tatcttttgg gactgatcct gggcgatggt
     7801 gctccaccaa aacagttggc cttctttctt tgccgccgca atcactttag gatcgcccgc
     7861 tgctccggca tgcggcgcta accacaaaat cagccaaata agcatcagcg ccggccatat
     7921 ccgctgtatc tgaatgaagt ccgccttcat acggaaattt tctcctgcgc atcggctatt
     7981 cctttgcgga acaatgcgtt gaagtccagg gaccttttgg ccgcggtcaa agccagttgt
     8041 tcctttccgc tcgtcgcata ctccgacagg aaaggtaaaa acatatcgct atgcttttcg
     8101 tcggcctggc tgtgcgcctg caagttggga atctgcttcc aggtaaaacc catctcctcc
     8161 atccattttt ttgccatgcg ggtggattgg ccgccgccga gatcgcccaa cagccggtca
     8221 tcgttgcacc actcggtgat cgtcatcgcc gctagtcctt caatccagct gagctcgctg
     8281 gtgatccacg accaggcata gagcacggcc cgcgtttcga gcaacggctc ggcattcagc
     8341 acttgcttgg gctccagacc gaccgacttt ccctgattga tgatcaaatg gagatgacca
     8401 tagtcggaga agtcatcgcg aatgatctcc tcgtattcgt gctcgagaat ccgttgtttg
     8461 acgcccagaa tcgggcagtt ggccgaaatg tgcgcccaac attcgcgccg gtgacgcacg
     8521 taaaggctct gctgcgtcac catgactgcg gcgcgcgctt tggtcatctt gatcgaaaaa
     8581 taatgttcga gctcgggcga tcgaatgtag tcgcgcacca tctcgcccat cgtctggtgc
     8641 cattgcgatt cgttcacgac ttgcctcctt tgtagagccg gtcgatgaaa ccttcctgcg
     8701 ccaaacgatc gacgatcgcg ttatcgaaga gctgcacctt gggcgggctc ttctggccga
     8761 ccatctccaa gacagtttcg atggcagccg gatcgacccg cggaattgca tccaggtact
     8821 tggtgacgaa ctcatagtgc tgttccgccg agccgccgcg ttgccccatg tacttctcga
     8881 gcaccttgag cccctgagct ttgcgggttt tgagcgccgc gatgccctct atataggatg
     8941 agacgattcg ctcaaccgtc ttgggtgatc ggcgatagaa atcgttactg acggccagca
     9001 ggttcatcga gaacgggata cccagctccg cggcgtccac caacatcttg gccttcgggc
     9061 ctggcgcctg cgaggacacc ctgccctccg cctggccggc gcgaaaaccg acgtcggctt
     9121 cgaccacccc gccgaactgg cgcaactcgg ctttaccttc cagattgagc ttgcgcatga
     9181 tcagccgggt gacgaaatcg ctggttgagc cgaaccgggt gatcgccaga gtcttaccct
     9241 gaagctgctc ggcgcgttga atatccggct gaacccaaag ctgcatacca gggcggctgg
     9301 tattgctgcc gactgcgatg atcggggctc ctttgatgat cgccgccacc actgcattac
     9361 tcgcgcccaa cgcggcatga acactgccgc ccaccaacgc ctggatcgcg gtcgcgccgg
     9421 aactgatatg aaccagctcg gcttgcaagc cgttacggtc gaaggcgccg atctctttgg
     9481 ccatccatat tccggacatg ctcgggctga tagcagcata ggcaaaagcg actttctctt
     9541 tgccagcgtc cgccgaagat gcgggttgcg atgaaatcag caccaacgtt agaatgagtt
     9601 tcgcaaacac aacaaaacgt aggatagaac cttttacttt tttaggtggt ttaaaatcgc
     9661 ccgggagccg cttcaacggg gcatggggac cgtaaaactt gattgactta ttcattgtct
     9721 gcctaaagca cttttatcat tggcgtcagt caggaagcgc ctgcatcgcg tcggcgagac
     9781 ccttctgata aagacggtac aactcttcgg attcttttgc cgcctgcaat atcatttctc
     9841 ggttctcgcc ggagccgaac tcctcccaaa tcggaagaaa catgtcgacg tgacccttgt
     9901 cggcttcccg gtgcgctttc caattcggca gatcgtccca ggtgatgccc agatacttga
     9961 cccagagatt tcccatgcgc gccgagtgac caccaccaag atccgccaag aggcgatcgt
    10021 cctgattcca ttcggtcatg gtcattgccg ccaaaccttc gagccagctt tttttcttgc
    10081 acatccaacc ccacgcatac agtgccgcgg ctgtagtcgg gatcggctta gtttgcaaaa
    10141 tatcctcggc cgttagtccg atcggttttc cctggcggat gattaaatct agatgaccat
    10201 gctcggagtg cgcatcgcgg atcatctctt cgtactcatg agcgagtatc ttctgcttga
    10261 ccgccaggac cgggcagttt cctgaaacat acgcccagca gtcgcgccga tgacgaacgt
    10321 acagagaaag ctgctttagg atcagctgtg ccctgggcct gcttatcgga atcgaatagt
    10381 atcgctccaa ctccggcgag gacatcgtcg cgcgagctaa ttcaccgaat gcttcgtgcc
    10441 aaccgttcgc agccatagta attttcctcg atctttagac aaccgcggcg cttgggttat
    10501 ggagtgtgcg ggcgagtctc tgaggccctc cgctaaacgc actttgccat gtcctgcgca
    10561 attcccgcgc ggaatatctt tagcagctcc atggactctt tggccgctcg ataggcaagc
    10621 gcttccttct cgccgatggc gaacttttcc aggtcttcca aaaacatgtc gctatgctcc
    10681 tcgtccgcct cactgtgaac tttgaaattc ggcatttgct cccagccaaa accaagatcg
    10741 tccatccagc gcttggccat gcgactggac agaccgccgc caaaatcctg gagcaaacga
    10801 tcatcattgg cccactcggt gatggtcaga gcggtcaaac cttcgatcca actcttctct
    10861 ctcgtcatcc atgcccaggc gtaaagagtg gcgaaagttg tcggcaaggg ttcgacttcc
    10921 aagacttcct ggggcgagag tcctaacgct tttccctgcc gaacgatcag gtccagatgc
    10981 ccatgctcgg tgtattcatc cttgatcacc tcgccatatt cgtgctgcag gatcttttgt
    11041 ttgaccgaca tctgcgggca gttgccggaa acgtgcgccc aacaatcgcg ccggtggcga
    11101 atgaacagtc ccaattgcgt gatcaagatt tgcgcgccgg gtttcgtgat tttgacccga
    11161 aaatagtgat cgagttccgg cgcgcgcatc gcatcacgca ccagctctcc cagctgccgg
    11221 cgccactcag ctcgcttcat gcggatctcc cgttacttgt aaagcctgtc gacgaaacct
    11281 tctttgataa tcgcatcgat gatgctgtta tcgaagaact cgttgggcga gctatccttt
    11341 ttgccttccc aatcgagcac ggtttgaatc accgccgggt ctgttctcgc gttgcgatcc
    11401 aaatatttca cggcataggc ataggcttcg tccagtgatt caccgcctcc gaggagatat
    11461 ttggctaaaa ctttataagc aaactctttt ctacttctaa gccccgccac tccttcgatg
    11521 taagctttga gcatggcctc aacgatccgc ggagaactct tgtagaaatc gcgtttgacg
    11581 gcgatcagat ccatcgaaaa aggaattccc atgtcgggca aatcaactaa catgcgcacc
    11641 tttggaccgg gcttcatgct ccgtacagcg ccggcaatca tcccggtccg aatagcggtg
    11701 tccacctccc gggtgccacc catgggttgg attttgactt tgtcctggag tcccaacttc
    11761 tcgatcacga actgtgtcaa gaaatgcgtc gacgagccat gccgggtaat gcccaggact
    11821 ttaccttgaa gctgctccgg tctggtgatc tcaggctgaa caaataaact catcgcgggc
    11881 cggttggtaa tgctgccaac tgcaaccaga ggcgcgccgc gcagaatcga gctgacgacc
    11941 gcgttgctcg cagccacgct catatcgaga ttgccgccta ccatcgcctg gaccgtaacg
    12001 ctaccggagc tgatgtagat tagatcggcc ttgacgccat gcttctcaaa agctcccatg
    12061 tccttggcca tccatactcc ggcaaagatc gggctgattg cggcgtaggc aatggttact
    12121 ttgggcggcg tctgcgccgt cgaaagtccg gacgccagca ccagaaaaaa cacaataagc
    12181 gctgcgtcga accgaactaa acatcgaaca atcgacatca ccgttatcac atgcgcctca
    12241 acctgccgcc cgcaaggcga gacccgatac tttgaagcaa caggtaacac aaggaaccgt
    12301 ctcaatgcag gctgctagac cggccagccg tgctcggaca cgtcgacgcg gttcccttcg
    12361 acatctttga tccagctttc cgcaatggct ccgtaggtat tgtcctcggc tttggtatcg
    12421 gcaattttct cgatctcttt gacgctttcg accttgaacc caaagtgatg gattccccat
    12481 ggatatttct cactatggat tagagcgaga tcgagatggc catcggatag ataaatagtt
    12541 cccccaggcc ggcgatgttt ttcttccatg ccgaaaactt tcttatagta ttctgcggtc
    12601 ttgtcgagat cttcgacgtt gattgcgata tgtcgaattc gtggtttttc catgacaatt
    12661 ctccttgtga aggtaacttg attaactgag tggtattttt tccatggccc gggccacgcc
    12721 ctcgcgatag agcgcgaaca gagcgagcga ctctttgact gcggcgagcg ccagttcttc
    12781 cttctcgccg gtggcatact ccgcaagaaa cggcaaaaac atgtcgctgt gctcttcatc
    12841 cgcctgtgaa tgcgcgacga agttcggcat gtcgcgccat gccagtccca gatcatccat
    12901 ccagcgccgg cccattcggg tagagtgacc gccgccgata tcgttcaaga gacggtcatc
    12961 attggtccat tcggtaaccg tcagggcgga taagccttcg acccagctct tagcgtgcgt
    13021 cagccaggcc caagcgtaaa gcgtcgcggt cgtgcttaaa aggggtttgg tgtcgacaac
    13081 ctcgcgggcg cttaagccga gcttttccgc ttggcggatg atcagatgaa gatggccgaa
    13141 ttctgaatac tgatccttga ttacttcgcc gaactcgtgc cttagaatgg cttgtttgac
    13201 tgccatcacg ggacaattgg cagataccag cgaccagcaa tcacgccggt ggcgaatgta
    13261 aagtcccagt tgcgtgatca tgagctgcgc ccgctgcttg ttcatcttga cggaaaaata
    13321 atattccagc tccggcgagc gcatgtgatc gcgcaccatt tcgccaatcg cgtttctcca
    13381 ctgctcaact tgcatccttg actcctcgac ctgcttgcct tatttacgcg gctttcagtc
    13441 cgtcatctgt ctccctatat ccgattatcg gtgcgcagct caaggcttct tgtaaagcct
    13501 gtcaacgaag ccttcgcgca ccattttatc taccatcgag ttatccgcga aggtctcgag
    13561 cggtatccct tttttgccca tgaattccag aatcgaatcg atagcttccg gctcaacccg
    13621 cggtgtcttc tcgagataca caatcgaatc ctgatagatt tcgtcgatct tcttctgatc
    13681 gcgcaagcgg gcatatttgg caatggcttt gtaggctcgc tccttctggg aatgaattgc
    13741 cgctacccct tccgtatagg cgcggaccat accgtcgacc acatgtggaa aacgctttag
    13801 atcgtcgcgc gaatgggcaa caacgtccat cgagtactga aaccccaggt cttcgagctt
    13861 gagcaatagc cggggctgca ttccggcatc gacacggagg cttgaggtga cggcgccggc
    13921 aatttgcttg ttttgaaatg ccgcgctgac ttcgatcgtg ccgcccatct gacgcacgct
    13981 gacggcattc tccaagccgt atttcttgag cagcatgcgg gttagattgt cggtcaccga
    14041 accaaagcgt gtcacaccga tggtcttgcc cttgagatct tccatgcggg taatctccgg
    14101 ttgaacccag aaccgatggt agggtcggtt cgccgtgctc aacaccgaca cgagcggggc
    14161 gccgcgcaaa accgccgcga tcacggcgtt ggtcgcggcg atgccgccaa cgatatcgcc
    14221 gccgagtaac gcttgcaccg tggtcggacc agacgagatg tagatcattt ccgcatcgat
    14281 gccgtatttc tcgtaggcgc cgatctcttt ggccatccat gagcccgcgg ccatggctcc
    14341 gactgtggaa tagccgaaga cgattttttt tgtttgcgcg gcaagctcgg caggcttgaa
    14401 tagcaaagcg cacaccgtga tcatcaaagc ggatctcaaa acgtctctta ataactgcat
    14461 cttttccctt tcgtgttgat gatggtagat caactaaacg atcgccatcg ggcaacgcag
    14521 cgccattaac ccacctgtct aaagagcctc gctcgaacga tcaattcgac gctgcaagat
    14581 cgactaggtt cattcccctg gatgcgatgc gccgtaggac gaaccggcga agctgacaag
    14641 gaaatcttgc tacaacacag gactgatcaa ctcacggccg aggaattcag ttaccagcgg
    14701 ccaggcaaga tccgccagat agggttggta gccatccgat tccgtcgaag caaagccgtg
    14761 attaccgaaa tgaaagtaat gccattccag cggctgtcca ttggcgtgaa agctttccat
    14821 caccttctgt tggatcgcga acgtgctggt tcgatcgttg ccgccgtaca aaaccattga
    14881 aggacacttg atctcacggg cggcatcgta cggatggcgc ggtctgatcg gtgttggcgg
    14941 ctcttcgcgc accgacgggt aataggcgac gaacgcgcgc accgccggcg tcgccgcgac
    15001 aaaatgaatt ccgatgcgcc cgcccatgca atagccgatg acaccgaccc gcgaccgatc
    15061 aacatcgttg cgtgaaaggc aatagcgcca tccctgatcg atgacgcgga cgaaatccgg
    15121 atcggctgtc ttgttttgga tttccgttcc cttgtcaatg tgggtttcgg cgggataacc
    15181 gagcatgtga tagagattgg gaacaactgt agtgtagccc aactgagcaa atctataagc
    15241 ggcggtcttg agatagccgg tgagaccgga gtgctggtgg atcaacaaca gcgccgggcc
    15301 tttctgcgtc tgctctggat gggcgatgta gccttcgatg ccgtccttct cctggtccaa
    15361 tctgcgagtt ttaatggtca tcatcgtttc ggctcccgat ggtcaggact tcttgtaaag
    15421 cttttcgaaa aagccctcac ggctaagctt ctcgacaatt gaattgtcct ggaaagtctc
    15481 gggggggatg ccccgtttgc ccatgaattc caggatcgtt tgcactgcct cgggttgagc
    15541 cagtgggata cggtccagat acgtgaccga gtcgcgatag tgatcttcaa tttgcttggg
    15601 atctgttaga tgcccatatt tggcgatgat ctttagcgag cgttccttgt tctgattcat
    15661 gaacgcgaca ccttcggcat aggcccggac aattccttca acggtttcag gactgccctt
    15721 caatgtatcg cgggatacgg cgagcatgtt catcgaataa ggaacaccca tgtcgaccag
    15781 attgaccagg atcttgggca ctgttagtga aggcccgcgc agctcaccgg tcaccgcgcc
    15841 ggcaatctga cgattctgga atgccgccgc cacttccatg gttccgccca tctggcgcac
    15901 gttaacgttg ccttcaagtc cgtgcttgcg cagcaagata cgggttaagt tgtcggtgat
    15961 cgatccgaac cgggtcacgc cgagagtttt tcctttcaga tcttcgagcc ggttgatatc
    16021 cggctgaacg aacagccggt gatagggccg gttggccgtt ccgccgacgg cgacaatcgg
    16081 cgcgccattt agaatggcat tgataaccgc attgctcgcc gcgctgcccc cgagcagatc
    16141 gccgcccagc aaagcttgca caaccaccgg accggaagag atgaaaatga tgtccgcctg
    16201 aatgccgttc ttctcgaacg cgccgatctc cttggccatc cacacgccgg tcgccatcga
    16261 tcccaccgaa gagtaggcga acttcacacg tgactgagct tccgcggcgg agtgaatatg
    16321 aacgccgaga aagatggaca ccgcaaacac cgcggttttg caaagcttcg atcccaggct
    16381 catggctgat cctttcgcac aaattataaa atatttgctc gagggcttga gaacctcagc
    16441 agcaacgaac agtacaatcg tcgaaggcct tcagtttact gtgaggctcg tgactgatga
    16501 ccgaaactcg caactcgcct ggcatctttg atcgccgccg gagacttttt ccggcgtctc
    16561 tggcagactt tattcgcggc ctggcaggcc aa
//
LOCUS       DASUVS010000091        15679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_468243, whole genome shotgun sequence.
ACCESSION   DASUVS010000091 DASUVS010000000
VERSION     DASUVS010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 15679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15679
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468243"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VF182_05105"
     CDS             <1..211
                     /locus_tag="VF182_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436487.1"
                     /translation="LLYIASGRLNAAGFVFASMLDRPLVPFAMAILWYLEVIPGPLAI
                     IFSIQDFGSFLWTLKTWRAESPKLN"
     gene            623..1522
                     /locus_tag="VF182_05110"
     CDS             623..1522
                     /locus_tag="VF182_05110"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HEX6436488.1"
                     /translation="MNQPILGFIGTGIMGKPMARNLIKAGYTLIVHNRSRAAVNELSK
                     EGAHGAANSNEVAARADIIITMLPDSPDVDLVYTGVNGILSGVKSGTLLVDMSTISPA
                     VARKLAGEAKKLSCDMLDAPVSGGEAGAISASLSIMIGGEAAAVERAMPIFESLGKNI
                     VHVGAAGAGQVTKAANQMVVGTTIAIVSEALVLAAKAGVDPAKVRQALLGGFAQSKIL
                     EVHGQKMLEHNFKPGFRIRLHEKDMKIALGAGFEYGVPLMVTSQVAQMMSAMKASGDG
                     DLDHSGLVKLIEQMAKVVLGHDK"
     gene            1549..1788
                     /locus_tag="VF182_05115"
     CDS             1549..1788
                     /locus_tag="VF182_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436489.1"
                     /translation="MSESANLLMLEFLSWISDRQRTYAEAMNAWQSTCPRHTIWEDAI
                     IDGLIQLNGLGTAHDPRVELTSRGKAYLNGDTPKQ"
     gene            1940..2938
                     /locus_tag="VF182_05120"
     CDS             1940..2938
                     /locus_tag="VF182_05120"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX6436490.1"
                     /translation="MAIKTMLTIICLLWISTNLAAESPKATVLVDLDPNSSAQTVIVE
                     SVASDPQGLIYVCDRVTGNVLRVDPKKPIPVIVGRVETRQVDGKTARADSSGLIFNEA
                     GDLYLTSGGFREILRIRKTDLNPEKPGVAETFATGTEGANGIAFDKKGNLYVSGGRNG
                     KIYRIGPTGGAAEDWAQIELHTRTLADGKTQQAIPANGLVFDSEGTVLYVADTARGAI
                     WKITIGSDGKPGKPSLMAQSALLEGADGPAFDSKGNLWVAANERNAIVEVTPDGKVRD
                     VIKNDSKGPLEFPTAVIFVGNTAYVSNFDTARRDNLAADGKTSLDGIGASIIQITP"
     gene            3274..4305
                     /locus_tag="VF182_05125"
     CDS             3274..4305
                     /locus_tag="VF182_05125"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6436491.1"
                     /translation="MKKIIFWFLVFLVWVPDARAQSPYYEGKTIRIVVGYPAGSAHDQ
                     WGRLIAPYLTKFIPGNPNAIVQNMAGAGSMVAANYIFGVAKPDGLSLGIVNAALYFDQ
                     LLARKEVQFDWSKFAWIGSSTPTNAMLYMWANTPYKTIHDVRTAVVPPKCGATGTGNT
                     GYYLPKLLEETIGAKFNIVMGYQGGADIDLAAEKGEVHCRAFTTTVFFAREPFHTWRK
                     NELVRVLVQAGQKREERLPDVPTLNELMDQYQTPDSNRRLAPVLLGSGGFGSAPTLSS
                     PGVQEEPLKTLRAAYAKAINHPDLIGEAKKKGLEAELISGEELQALAKEVIVQPPEVI
                     ARIKKIIGD"
     gene            4574..5113
                     /locus_tag="VF182_05130"
     CDS             4574..5113
                     /locus_tag="VF182_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6436492.1"
                     /translation="MSRLPVIDREALEPEDQAVWDRIAAVRTGVRGPFGVLMHVPALA
                     DRVAALEDYFRFNAALPAVDRELVIMATAREMNARYPWARHEARGREVGTRTEAIEAV
                     RGNGTLERLTARERLLVEIVRSLLRTHALPEALYTQALAELGRQQLIEAVTLAGHYSL
                     IGLAVNAFDVAPPDDRSTF"
     gene            5368..6387
                     /locus_tag="VF182_05135"
     CDS             5368..6387
                     /locus_tag="VF182_05135"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX6436493.1"
                     /translation="MRTDDLRIVRTRPLLAPAILAEEIPLTEIASTRVYETRRAIEAI
                     LDGKDRRLLVVVGPCSVHDTKAALEYATRLKPVADALSDALLVVMRVYFEKPRTVVGW
                     KGLINDPDLDESYHINKGLRLARQLLADLLQLGMPAGSEFLDTTFGQFYADLISWGAI
                     GARTAESQIHRELASGLSMPVGIKNRTDGNVQIAVDAIQTARSRHLFPSLTKEGAPAI
                     LETTGNPYAHLVLRGGSETGPNFDAASIESAAQLLLGAGLPEAMMIDCSHGNSEKDPR
                     RQVEVAAAIMGNLRNSPVRALMLESHLVAGRQNAPITYGQSITDACLGFEETENLLYR
                     LADAV"
     gene            6462..7106
                     /locus_tag="VF182_05140"
     CDS             6462..7106
                     /locus_tag="VF182_05140"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX6436494.1"
                     /translation="MKINSLLILACIAASIWAWQQDPAIAERNLIFSLGNFENGRVWT
                     LVVALFVHANVFHLVGNMLFLFVFGNTLEKSVGAEKLLVIFFTGGLLSFILSPAFLPR
                     DTGMLGASAAIFALAGSVMLIRPLKFSWLFLAPQGLIAIIYFIYNVVLVYEKSRIPGY
                     DPQVAYVAHIIGFITGIPFGIALSNRWKLNFLITLLLLAIYLAIVSGAAKSLFR"
     gene            7342..8109
                     /locus_tag="VF182_05145"
     CDS             7342..8109
                     /locus_tag="VF182_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6436495.1"
                     /translation="MNSKAKYFDCHFHIIDKNFPVVANQGFMPDAFTSEDYLARLKEV
                     DLCGGAVVSGSFQAFDQSYLFHALRVLGPSFVGVTQVPQTVSDQELQELNRAGVRAVR
                     FNVKRGGSEEIQHLERMARRVHERVGWHTELYVDSTELADLFETLVSLPAVSVDHLGL
                     SRSGFPTLLKLAERGIRVKATGFGRIDFDVRSALSDLYAANPRALMFGTDLPSTRAPR
                     PYQDEDYHLVMETLGEEKAANVFYRNAIEFYRPAKVV"
     gene            8201..9016
                     /locus_tag="VF182_05150"
     CDS             8201..9016
                     /locus_tag="VF182_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6436496.1"
                     /translation="MAQHINGPLYYERMGRTGPVIAFIHPNPMDQSCWIFQMAHFSTW
                     YRCIAIDIPGYGRSPKAESGLTMDDMAEACWEAVDEVSPGDEAILVGCSVGSSLAPHM
                     YHLRPDKTAALVLAGTGYNPTKEFTKGRIANYTANGVDYRWAYTFEDLSAAFRPTPLA
                     HFFANLFTERNQHADVQTIIHQFQALAQPYPEGHHERIACPTIILTGSEDNSHQRAFA
                     LQARIPNCELRTLPGAGHACQIEQPWLFDRFMIEFLAKHGLFLGRSTPAKTIV"
     gene            complement(9028..9921)
                     /locus_tag="VF182_05155"
     CDS             complement(9028..9921)
                     /locus_tag="VF182_05155"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6436497.1"
                     /translation="MRVILVVILMITSIAGRSEAAQSETASVLSPTQRVTLKWLGTAG
                     WEIQIGETKILIDPFLTRQQAVASEEWKTDEEAVLNVINGADYIFAGHSHADHIGDIP
                     FIAKRFGAKVIGSRTTVNLSLSASVDKAQLTTISGGEKLDFKDFSVQVIKSQHGILTR
                     DGRKGQPRSAEITRPLAGPILGKHFVEGGSYLYYFTFGKLRLLHQSTGNFIAENLQGI
                     EPDVAILAENSNYDWPGAIKILRPKTVIVHHYDDWHVPFSGGMTAAVRRRAQRLERDV
                     KSVDRNIKVIIPEFFQTITLE"
     gene            10103..11590
                     /locus_tag="VF182_05160"
     CDS             10103..11590
                     /locus_tag="VF182_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEX6436498.1"
                     /translation="MNHVSNNPGDQRFAFDNTYARELEGFYVPWKAAQVAQPRMIKFN
                     HQLAEELRLDANALDSDEGARIFAGNETPEGAVPLAQAYAGHQFGGFVPQLGDGRALL
                     IGEVIDRYGRRRDIQLKGSGRTPFSRAGDGRATLGPVLREYLIGEAMHALGIPTTRAL
                     AAVVTGEPVYRERALPGAVLTRVAASHVRVGTFQFFAVRGEQAKVRQLADYVIDRHYP
                     ELKGQANPYPGLLERVCDKQAALVAGWMHVGFIHGVMNTDNMTISGETIDYGPCAFMD
                     HYDPATVFSSIDTQGRYAYANQPKIAQWNLARLAETLLPLIDDDTNRAIARATEVVNA
                     FSGHYERYWVKGMRAKLGLLTEEEADLNLGTEFLTAMDGNNVDYTLAFRYLADAALGQ
                     QERVRGLFSDPSAYDRWSGHWQARLSREAVAPLERAQAMRRANPMFTPRNHRVEEALG
                     AAVEHDDYAPFEALLKILARPYDDQPEFAAYAEPPVEGQSHYRTF"
     gene            complement(11732..12616)
                     /locus_tag="VF182_05165"
     CDS             complement(11732..12616)
                     /locus_tag="VF182_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436499.1"
                     /translation="MLQVLPFSIFAFNSQEISITRNEAGWFARAFCFATSFIGVAALA
                     CSAFFAHVIAVMLLFLAINEYEGRKRLWLIGGLIGLATATRLPNGLNILFFMLAVSLG
                     VGTIQERAAELLKLLLPFAFLVGLLALYNFARFGTPLESGYTYQLNGFGMPYAPWNVP
                     GNTAGSALSFSYIPEHLWIFLFGPPSISAVGTSALLVSPFLFYLISVRWDLINKLIAS
                     NSALVLLTVLAFRSTGFEQVGYRLSLDFLPFVFWLLMRSRLQMNNRFKALICLALVVR
                     RMSDGILLVNRRKPSTGR"
     gene            complement(13044..14369)
                     /locus_tag="VF182_05170"
     CDS             complement(13044..14369)
                     /locus_tag="VF182_05170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX6436500.1"
                     /translation="MYNELLFSSLRHRRNIPTPINPTILVTQRHRLFKLVFVPLLVFS
                     LFGQCAVVWTQLDDIQAGYFDFVLYYSAAKIINDGNGAELFDLEVQRKYQKSFGGDDR
                     DWDLPFNHPPYELLPLLPLVEYPFPVAHALWTALSVFLLALALVRLFSFMETTHRSLF
                     ILMVLAFFPTITALKMGQDSLITTLLLVETFVSLKYNRYAIAGGLLALGFYKPQFVLP
                     LFGILLIHRRWASVLGFMSVGLLLGALSLVMVGRDGLMGLVTLWLPMINRGHVVWPEL
                     MMNLRGLIYIVLHPAGLTEATNVLTLGFSVATYAAMLQLWPRSEEEKDELFDLRFAAA
                     VIMTTLVSFHSYSYDGTMLVIPLVIMLNQVLKAPQPYSVRHRTFLAILISWFVPLLPN
                     VLLSAALLAWWALPLPLLFAVIAIEIRRRSAERCSLIKTEDSILVACKF"
     gene            14742..15524
                     /locus_tag="VF182_05175"
     CDS             14742..15524
                     /locus_tag="VF182_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009386106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HEX6436501.1"
                     /translation="MAQKFYVVWSGRQTGVFTDWATTQRAVEGYAGARYKSFSSRAEA
                     EQAFHAGVPANTPPERSSGQRPRASAKERRAAHIAHRFHITIYCDGACEPNPGHAGSG
                     IVIYRAGKLAELWYGLYNPVGTNNTAELNALYHALRMAEAELEAGNSVEVCSDSAYSI
                     NCIRLWAPGWEKRGWTKTGGAIKNLKIIQDCYAIYRRIKEKLTLTHVAGHVGTEGNEL
                     ADRMAMLAVRSKQTELRLYHETSDIPTLLKRFRARPAQPLTN"
BASE COUNT         3736 a         4287 c         4252 g         3404 t
ORIGIN      
        1 cctgctttat atcgccagcg gtcgtttgaa tgccgcggga ttcgtcttcg cgtcgatgct
       61 ggacaggccg cttgtgccgt tcgcgatggc tatcttgtgg tatctcgaag ttatccccgg
      121 acccctggca atcatttttt cgattcaaga tttcgggagc tttctatgga cactcaaaac
      181 ctggcgcgcc gagtcaccca agctgaattg aatcgctggc tgtgcgctgc cggtaaaacg
      241 ttttgccccc taagcataga gccgcggagc gttgcgatcc cggcgtaaac aaagccgcaa
      301 cattagcctg agattccaga tcgtcatcag caattcaaca attcaaatac tgctacagcc
      361 acatcccacc acatatacag agagcactcc tttctgttat atctttaaga gctcgtcgat
      421 gaacttgcga tcgatttgct tgaggagcgt gatgttaatg aatcctgcgg cttcgcgacg
      481 caattgactt gccatcagcc ctctcgagta ataatcctcg taaccaggag ttcttaacgc
      541 acgaagtttt ccggcaaagt tgtttgccac gacttaaaaa cggcctgatg gtttaagctg
      601 gccgatcgta gaggagctga tcatgaatca gccgattctc ggtttcattg gcacaggcat
      661 catgggcaag cccatggctc gcaatctaat caaagcaggc tacaccttga tcgtgcacaa
      721 tcgcagtcgc gcagcggtga acgaactgag caaggaaggc gcacacggtg cggcaaattc
      781 aaatgaggtc gcggcgcgcg ccgatatcat cattaccatg ctgcccgatt cgcccgatgt
      841 cgatctcgtt tataccggcg tgaacgggat tttgtcgggg gtaaagtcgg gaactctgct
      901 tgtcgacatg tcgaccatct cacctgccgt cgcgcgcaaa cttgccggtg aggcaaaaaa
      961 gctaagctgc gacatgctcg atgcgccggt gagcggcggc gaagccggtg cgatcagcgc
     1021 gagtttatca attatgatcg gcggcgaggc agccgcagtc gagcgggcga tgccgatctt
     1081 cgaatcattg ggaaagaaca tcgttcacgt cggcgctgcc ggggccggcc aggttaccaa
     1141 ggcggctaat caaatggttg tcggaacgac cattgcaatc gttagtgaag ctcttgtact
     1201 cgccgccaag gccggggtag atccggcgaa agtgcggcaa gcgctcctgg gcggttttgc
     1261 gcaatcgaag attctcgaag tccatggcca aaaaatgctc gaacataatt tcaaaccggg
     1321 ctttcgcatc cggctacacg aaaaggacat gaagattgca ctcggggccg gattcgaata
     1381 cggcgtaccg ctgatggtta caagccaagt cgcgcaaatg atgagcgcga tgaaagcctc
     1441 gggtgatggc gatcttgatc actccgggct ggtaaagctc atcgagcaaa tggccaaggt
     1501 cgtgctgggt cacgataagt agatacgata ggcgagcacg gaggctatat gtcagagtcg
     1561 gcgaacctct tgatgttgga gtttctttca tggatatcgg atcgtcagcg gacatacgcc
     1621 gaagccatga atgcatggca gagcacgtgt ccgagacata ccatttggga ggacgcgatc
     1681 atcgatggcc taattcaact gaacggcctc ggaacagccc acgatcccag agtcgagctc
     1741 acgtctcgcg gaaaagctta tcttaacggg gacacgccta agcagtgagc ctgtgatctg
     1801 ctttgcgact gtctatggga gcaaaacatc atcactgaac gaagacttga ctcaattcag
     1861 gcgcgatcaa tgaatttttt tgatagtttg tacctgcttt agtgaccaag ccgtacaact
     1921 gcgttgacgg agaaaagtaa tggcgatcaa aacgatgctt acgataattt gccttctctg
     1981 gatctccacg aacctggccg ccgagtctcc caaagcaacc gtattggtcg acctggatcc
     2041 caacagctca gcgcaaacgg taattgtcga gagcgtcgcc tcagatccgc aggggttaat
     2101 ctacgtctgt gatcgggtaa ccgggaacgt actgcgagtc gatccgaaaa aacccattcc
     2161 ggtgatcgtc ggtcgtgtcg aaacgcgaca ggtggacggg aaaactgcga gagccgacag
     2221 ttccggacta attttcaacg aagcgggcga tctctatctg acgagcggtg gctttcgaga
     2281 gatcttgcgg atcaggaaaa cggacttaaa tccagaaaaa cccggcgttg ccgaaacttt
     2341 tgccaccggc accgaaggcg ccaacggcat cgcattcgat aaaaagggaa atctttatgt
     2401 ctcgggagga cgcaacggca agatctatcg catagggccc acgggagggg cggctgagga
     2461 ttgggcgcaa atcgagctcc acacgcgcac gttggctgac ggcaagaccc agcaggcaat
     2521 tccggcaaat ggactggtct tcgacagcga aggaacggtt ctctacgttg ccgataccgc
     2581 gcgcggcgcg atttggaaaa taacaatcgg ttccgacggc aagccgggca aacccagtct
     2641 gatggcgcaa agcgcattgc tagagggagc cgacggtccc gccttcgatt ccaaggggaa
     2701 tctctgggtc gctgccaacg aacgtaacgc cattgtcgag gtcactccag acggcaaagt
     2761 gagggacgtg atcaagaacg acagcaaagg gccactcgaa tttccaactg cggtcatctt
     2821 cgtcggcaac acggcctatg tcagcaactt cgacacggca cggcgcgaca atctcgcggc
     2881 ggacggcaaa acatctctcg atggaatcgg tgcatcgatt attcagatca cgccgtagga
     2941 ttttactgct aattgataag agcttcgcac tcacaaagct cgctcggcga atactcgcgt
     3001 atcaacccgt tctggcgcag cggcgacacc aattccgtcc caggcttgag catttaccag
     3061 cggtgatttc ctgcaagctt ttgcgaccgc caaatccgat cctcatatag cactccgaga
     3121 gacaccagct atttcttggg ctttaacaaa tacacttacg cccattggtt ccttcagctt
     3181 tgttggctta ccgctcttat tttcttgtct tgtctttcca catctgttag tccggcgtgc
     3241 attaggcagg gataagtcca actcggaggt gccatgaaga agataatctt ctggttcttg
     3301 gtgtttctcg tttgggttcc cgacgccaga gcgcagtcgc cctattatga gggcaaaacc
     3361 atacgaatcg tggtcggcta ccctgcgggc agcgcccatg atcagtgggg cagactgatc
     3421 gctccctacc tgaccaagtt cattccggga aatcctaacg ctatcgtaca gaatatggcc
     3481 ggggccggtt cgatggtggc tgccaactac attttcggag tcgccaagcc cgacggattg
     3541 agccttggaa tcgtcaacgc ggcactctat ttcgaccaac tgttggcccg caaagaggtc
     3601 cagttcgatt ggtcaaagtt cgcctggatt ggcagctcga cgccgaccaa tgcgatgctc
     3661 tacatgtggg ccaatacgcc ttacaagaca attcacgatg ttcgcacggc cgtcgtgccg
     3721 cctaagtgcg gcgccaccgg gacgggcaat accgggtact acctgcccaa attgcttgaa
     3781 gagaccatcg gcgcgaaatt taacatcgtc atgggttatc agggcggcgc cgacatcgac
     3841 ttggcggcgg agaaaggcga ggttcactgc cgcgcgttta ccacgacggt cttttttgcg
     3901 cgcgagccgt ttcatacctg gaggaaaaac gaactggttc gagtcctcgt tcaagcgggc
     3961 caaaagcgcg aggagcgtct gcctgacgtg ccgacgttga atgagttgat ggatcagtat
     4021 cagacccctg attcaaaccg ccgtctagcc ccggtattgc ttggctcggg cggctttggc
     4081 agcgcaccga cattgtcctc tcctggtgtc caggaggaac cactcaagac tctacgcgcc
     4141 gcctacgcca aggcaataaa ccacccggat cttataggcg aagccaagaa gaaaggactc
     4201 gaggcggagc tgatcagcgg cgaggagttg caggctttag ccaaagaagt gattgtgcaa
     4261 ccgcccgaag taatcgcgcg gataaagaaa atcattggag actaaaagtt cagccgaaga
     4321 acggagtgct aaagtattgg agtgctgaga ttggcgttga acatgactcc attattccac
     4381 cactctaata ctccaacttc cttagacaat cggccatgag gaagcagtcg agtctctgca
     4441 gcgaacctgg aagcggcctg ctcaaaattt caatgcaaag ggcagataat gaacacgggc
     4501 aagcctactg acctactttg tagcgagctc atcgcagcgc agttgcgttt ttcactagcc
     4561 gcatggagga gtcatgagcc gattaccagt tatcgaccgt gaagcgctgg agcccgagga
     4621 tcaagcggtt tgggaccgca ttgctgctgt ccgcacggga gtgcgcgggc cctttggagt
     4681 attgatgcac gtaccggcgc tcgccgaccg agtcgcagct ctggaagact actttcgttt
     4741 taacgcggcg ctgccggcag tcgaccgtga gctcgtgatc atggctacgg cgcgcgaaat
     4801 gaacgcgcgt tacccgtggg cgcggcacga ggccagagga cgtgaagtgg gcacaagaac
     4861 cgaggcgatc gaagcggtcc ggggtaacgg cacgttggag cgattgaccg cgcgcgagcg
     4921 cctgctcgtg gaaatcgtac gctccttgct gcgcacgcat gccttgccgg aggcgttgta
     4981 tacgcaagcg ttggccgaac tgggccggca acaactgatc gaggcggtaa cgctcgcggg
     5041 tcattacagc cttatcggat tggcggtgaa cgcattcgat gtcgccccgc cggatgaccg
     5101 ttcgaccttc tgaggggcgc gccccgcttt acttcacgga tcagcgcccc ggccccgccg
     5161 cgcttgatct ccagcgctcc accgggcgtg tgaaacaaaa cgctgcgatc atcacacgcg
     5221 gttgacacaa tttcggatag tcgagtactc tttccaagat gagcggtggc tgttcgatta
     5281 tttcccgggc agcttgtaag attcctattt gtgcgatgac accgatttat aagaaaatcc
     5341 gggttctttc tagctggcag ttgagccatg aggactgacg atcttcgcat cgtcagaacc
     5401 cggccactgc ttgcgccggc catactcgcc gaagagattc ccctcaccga gatcgcgtcg
     5461 acacgagtct acgaaacgcg ccgagccata gaggcaattc tcgacggcaa ggataggcgg
     5521 ctcctggttg tcgtcggccc ttgctccgta cacgacacca aggccgcgct cgaatatgca
     5581 accagactca agcctgtcgc tgatgcctta tccgatgcgc ttctggtcgt catgcgagtc
     5641 tacttcgaaa agccgcgcac cgtggtcggc tggaaaggtc ttatcaacga tccggatctg
     5701 gacgagtcgt atcatatcaa caagggattg cgccttgccc gccaattgct cgctgatctt
     5761 ttgcagttag gaatgcctgc cggcagcgag tttctcgata ctacctttgg ccagttctat
     5821 gccgacctca tcagctgggg tgcgattgga gcccgcacgg ccgagagcca gatacatcga
     5881 gagctggcat cgggtttgtc gatgccggtg ggaatcaaaa atcggacgga tggcaatgtg
     5941 cagattgcgg tcgacgcgat acagactgcg cgcagccggc atctgttccc atcactcacc
     6001 aaagaaggcg cgccggcgat tctggaaact accggcaatc cctatgccca tctggtcctt
     6061 cgtgggggca gtgagacggg gcccaacttc gatgccgcct ccatcgaaag cgccgcccaa
     6121 ctgcttcttg gcgccggcct tccagaggcc atgatgatcg actgcagcca tggtaacagc
     6181 gaaaaagatc cccgccggca ggtcgaagtg gccgccgcga tcatgggcaa tctcagaaat
     6241 tcaccggttc gagcgctgat gctcgagagc catctcgtcg cgggacgtca gaatgcaccc
     6301 ataacttatg gtcagagcat taccgatgca tgtctgggtt tcgaggaaac ggaaaacttg
     6361 ctataccggc tcgcagatgc ggtttagcga tcacgactat ggccctcact ctttcctgag
     6421 tgggttactc gcactgtagc tccctctctc ttgtcgatca cttgaagatc aactcgctgc
     6481 tgatcctcgc ctgcatcgca gccagcattt gggcatggca gcaggaccca gcgattgccg
     6541 aacgaaatct catcttcagt ttgggcaatt tcgaaaacgg ccgagtctgg acgctggtgg
     6601 tggccttgtt tgttcacgcc aacgtctttc atctggtcgg caacatgctt tttctatttg
     6661 tcttcggaaa taccttggaa aagagcgtcg gcgcagagaa actcctggtg atttttttca
     6721 ccggcggcct tctcagtttt attctgagcc cggcgttttt gccccgcgac accggaatgc
     6781 tgggggcctc ggccgcaatt ttcgccttgg cagggagcgt gatgctcata cgaccgctta
     6841 aattctcctg gctctttctc gcgccgcagg ggctgatcgc cattatctac ttcatctaca
     6901 atgtcgtctt ggtctacgag aagagccgga tacccggata tgaccctcaa gtcgcttatg
     6961 tggcgcacat catcggtttc attaccggaa tcccatttgg aatcgcgctg agcaatcgct
     7021 ggaaactaaa ttttctgatc acactgctac tgttagcaat ttacctagcg attgtgagcg
     7081 gcgcagcgaa atccctgttt cgctagacag actcgggacg ggtcaagaca accaccgccc
     7141 ggtcaatctt gaattttctt ctcgacgcag aaaacgaatc cgattttcat atctccggcc
     7201 gtggtggaat ttgtgatagt tatgcggctt gaacttgcca ccatcaagct gatcgaatat
     7261 accgaggggc gataggaaac tttaccagca tcattcgcta gcaccgtcac acttatcgat
     7321 agacttgaga ggagatcatc gttgaactcc aaagcaaagt attttgattg ccactttcat
     7381 atcatcgaca aaaattttcc cgtcgtggcc aatcaaggat tcatgcccga cgcgtttacc
     7441 agcgaggatt atttagcgcg cttgaaggag gttgatcttt gcggcggcgc ggtggtctcc
     7501 ggctcgttcc aggcttttga tcagagctat ctattccatg ctttaagagt gctgggccca
     7561 tcttttgtcg gcgtcacgca agtgccccag accgtgtccg atcaggaatt acaggaactc
     7621 aaccgcgcag gtgttcgcgc tgtccgcttc aatgtcaaac ggggtggatc ggaggaaatt
     7681 caacatttgg aacggatggc gcggcgtgtt catgaacggg tcggctggca caccgagttg
     7741 tacgtcgact ccaccgagct tgccgatctc tttgagactc tggtctcatt gccggcggtc
     7801 agcgtcgacc atctcggtct ctccagatcg ggttttccga cgctgcttaa gctcgccgaa
     7861 agaggtatac gggtaaaagc caccggcttc ggacggatag atttcgatgt ccgttcggcg
     7921 ctctccgatc tctatgcggc caatccacgg gcgctcatgt tcggcaccga tctgccgtcc
     7981 acccgcgcgc cgcggccgta tcaggatgaa gattatcact tggtgatgga gacattaggc
     8041 gaggaaaagg cggcgaacgt gttttacagg aacgccatcg agttttatcg cccggcgaag
     8101 gtcgtttagg aagcccgtcg ggcggcctcg tcacccgacg ggcactgaac tgattagtga
     8161 cgaaatgctt agagacgcac atcaacactg agggagtcac atggcgcagc acattaatgg
     8221 acctctttac tatgaacgga tgggacgcac gggtccggtg attgccttta ttcatcccaa
     8281 tccgatggac caatcgtgct ggatttttca gatggcccat ttctcgacct ggtaccggtg
     8341 tattgccatc gacataccgg gttacgggcg ctcgcctaaa gcggagtccg ggctgaccat
     8401 ggacgacatg gccgaggcct gttgggaggc ggtcgatgaa gtaagccccg gggacgaagc
     8461 gattctggtt ggctgctcgg taggctcgtc gctcgcgccc cacatgtacc atcttcgccc
     8521 cgacaagacc gccgcactcg tgttggccgg cacgggttac aatcctacca aggaatttac
     8581 caagggacgg atcgccaact atacggccaa tggtgttgat tatcgttggg cctatacgtt
     8641 cgaagatcta agcgccgctt ttcgccccac tccgctggcg cacttctttg ccaatctctt
     8701 caccgaacgc aaccagcacg ccgatgtaca gaccattatt caccaattcc aagcgctggc
     8761 gcagccctat ccggaaggcc atcacgagcg cattgcctgc ccgacaatca ttctgaccgg
     8821 cagcgaagac aacagccatc agcgcgcgtt cgcgctccaa gcccgcatac caaattgcga
     8881 gttgagaaca ttaccgggcg ccggccacgc ttgccagatc gaacaaccct ggctattcga
     8941 tcgctttatg atcgagtttt tggccaagca cgggctattc ctgggacgct ccacgccggc
     9001 taaaacgatc gtctgagcgg cgcagtatta ttccaacgtg atcgtctgaa aaaactccgg
     9061 aatgatcacc ttgatgttgc gatccacgga cttgacatcg cgctctaagc gctgggctct
     9121 tcttctgact gccgccgtca tgccgcccga gaacggtacg tgccaatcgt catagtggtg
     9181 gacaatgacc gttttgggac ggaggatttt gatcgctccg ggccaatcat agttgctgtt
     9241 ctcagcaaga atcgcgacgt ctggttctat tccttggaga ttctctgcga tgaaattgcc
     9301 tgtgctttgg tgcagcagcc ggagtttgcc gaaggtgaaa taatagagat aacttccgcc
     9361 ctcgacgaaa tgttttccga gtatcggccc cgcaagcggg cgggtaatct ccgcggatct
     9421 gggttggcct tttcgaccat cgcgggtgag tatgccgtgc tggcttttaa tcacttgcac
     9481 cgagaagtct ttgaaatcca atttctcgcc gccgctaatg gtcgtcaatt gggctttatc
     9541 gacgctggcg ctcaacgaca gattcacagt ggttctggag ccgatcacct tggccccgaa
     9601 gcgtttggcg atgaacggga tatcaccgat atgatcagcg tgactgtggc cggcgaagat
     9661 gtagtcggcg ccgttgataa cattaaggac cgcctcttcg tcggtcttcc attcttccga
     9721 ggcgaccgcc tgctggcgcg tgaggaacgg atcgatcagg attttggtct caccaatctg
     9781 aatttcccaa ccggcagtac cgagccactt cagggtcacc cgctgcgttg gcgacaaaac
     9841 ggaggcagtc tccgattgtg cggcttcaga ccggccggcg atcgaagtga tcatgagaat
     9901 gaccactaag ataactcgca tgatgatcct ccacgctgga gctatgcgta agtcacacgt
     9961 gacagcttgc tccagataca gcaactaaac gcgccagctc attgaaccga gcataccttc
    10021 tgagaggtct agatattaat atcgttatcg agaacaatga tagttgagaa tcaacaaatc
    10081 tcctgacgaa ggaaataaca gtatgaatca tgtttctaac aatcctggag accaacgatt
    10141 cgccttcgac aacacgtatg cccgcgaact cgagggtttt tatgtcccgt ggaaagcggc
    10201 gcaagtggcg cagcctcgga tgataaagtt caaccatcag ctagctgaag aattgagact
    10261 ggatgcgaat gctctcgatt ctgatgaagg cgcgaggatc tttgccggca acgaaacccc
    10321 cgagggagcc gttcccttgg cgcaagccta tgccgggcat cagttcggcg ggtttgtgcc
    10381 tcaactcggc gatgggcgag cgctgctgat aggcgaggtg attgatcgtt acgggcgccg
    10441 ccgtgacatt cagctcaaag gctcgggccg cactccgttt tcgcgcgcag gcgacggtcg
    10501 ggcaacactc ggacctgtat tgcgcgaata tctgatcggc gaagccatgc acgcgcttgg
    10561 catcccgacg acgcgagcgc ttgccgcagt ggtcacaggc gagcctgttt atcgcgagcg
    10621 agccctgccc ggcgcagtct tgacgcgcgt tgctgccagc cacgtccgtg tcggcacctt
    10681 ccaatttttc gccgtccgcg gtgagcaggc caaggtgcgg caactggcgg attatgtgat
    10741 cgatcgccat tatccggagc tgaagggcca ggccaacccg tacccgggct tgctcgagcg
    10801 ggtgtgcgac aaacaagcgg cgctcgttgc cggctggatg cacgtaggct tcatccatgg
    10861 tgtgatgaac accgacaata tgacgatctc cggagagacg atcgactatg gtccctgcgc
    10921 tttcatggat cattacgatc cagccaccgt ctttagctcc atcgacaccc aggggcgcta
    10981 cgcgtatgcc aatcagccaa aaattgcgca gtggaacctg gcgcggctgg ccgaaaccct
    11041 tctgccgctc atcgatgacg atacaaaccg cgccatcgcg cgcgccactg aggtggttaa
    11101 cgccttctca ggacactacg aacgctactg ggtaaaaggc atgcgcgcca agctcggatt
    11161 actcacggaa gaggaggccg atctcaacct tggaactgag tttctcactg ccatggacgg
    11221 caacaatgtc gactatacct tggcgttccg ctacctggcc gatgcggcac taggccagca
    11281 ggaacgggtc cgaggcctct tttccgatcc gtcggcctac gatcgatgga gtggacattg
    11341 gcaggcccgg ctctcgcgcg aagcggtggc acctttggaa cgcgcgcagg cgatgcggcg
    11401 cgcgaatccc atgttcactc cgcgcaatca tcgagtcgag gaagcgttgg gtgctgcagt
    11461 ggaacatgac gattatgcgc ctttcgaggc gctgctgaaa atcctggcgc ggccgtacga
    11521 cgatcagccg gagttcgcgg cctacgccga acctccagtg gaagggcaaa gtcattaccg
    11581 aaccttctga ggtacctgag tcacgcgtcc gggggacgca acgatttcac cgccacattg
    11641 aacgggtaga tctggcggag gttttcgccg caaatttgga gctatgccca atcatcgacc
    11701 tcgtcactcc atcaatgcct cataactcga actatctccc tgtcgacggt ttacgccgat
    11761 tgacaagtaa aatgccgtca gacatacgtc gaaccacaag cgccaggcaa atcagcgcct
    11821 tgaatcggtt gttcatctga agtcgcgaac gcattaagag ccaaaaaaca aacggcaaga
    11881 agtccagtga aaggcggtaa ccgacctgtt cgaagcctgt cgacctaaaa gccagcaccg
    11941 tcagcaagac cagcgcgctg ttcgaagcaa tgagcttgtt gatcaagtcc caccgcactg
    12001 aaattagata aaaaagaaac ggtgagacta ataaggcact tgttccgact gcactaatgc
    12061 tgggaggccc aaacagaaaa atccatagat gctccggaat gtatgaaaaa ctaagcgctg
    12121 atcccgccgt gtttccaggt acattccacg gcgcataggg cataccgaaa ccgttcagtt
    12181 ggtaggtgta acccgattcc agcggagttc caaagcgcgc gaagttgtag agagccagta
    12241 gtccaacgag gaacgcaaac ggaagcagta atttcaaaag ttcggcggct ctttcttgaa
    12301 tcgtcccgac tcctaaagat acggcaagca tgaagaacag gatgttcaac ccgttcggta
    12361 gccgggtcgc cgttgcaagc ccgatcaaac cgccgatcag ccacagtcgc tttctcccct
    12421 cgtattcgtt gatcgctaag aatagcaaca tcacggctat gacatgagca aagaacgcac
    12481 tgcaggccag cgccgctacg ccgatgaatg acgttgcaaa acaaaatgca cgtgcaaacc
    12541 aaccagcctc gttgcgagta attgaaattt cctgcgagtt gaaagcaaag atagaaaacg
    12601 gcaagacttg caacaaaaga cagtaccccc tgatggaaat atccggccca aaccagcgac
    12661 atgcttaaga ttgccggaga tggaccaaat ggagagtagt gatgcccttg gaataacgca
    12721 gtgtcccccc aactcagtgg cacgctcgga agcaaaacca acttccattg aaggaacgat
    12781 tcgccagccg gctggtgtgt tgatcatccg acgtattgag gtttagccaa gcgatcacgc
    12841 caaataaggt gacggcgcac acaaacggca gtacacgtgt tatttttatg atcggtagtt
    12901 ttgcatctgc cgtctttgcc accttacgac tccgccccat ctccatcatt agaagaagca
    12961 gcgttcaatc ttccttcatg cgcggctagt gccatgaggc tcgtctcgaa gcctgctctg
    13021 gttcgcggtc ttgtagcagc aacttagaat ttgcaagcaa cgagtatcga atcttcggtt
    13081 ttgatcaagc tgcatcgctc ggcagagcga cgccggatct cgattgcgat cacggcaaaa
    13141 agaagcggta agggaagcgc ccaccaggcc agtagggcgg cgctaagaag cacatttggc
    13201 aacaaaggga cgaaccatga aatcagaata gccaagaatg tccgatgccg cacagaataa
    13261 ggctgcgggg ctttcaggac ttggttcagc attattacaa gagggatcac cagcatagtg
    13321 ccatcgtagg agtatgaatg gaaactcacc aacgtcgtca tgataacggc agccgcaaag
    13381 cgcagatcaa acaattcatc cttttcttcc tcgcttcgtg gccacagctg caacattgcg
    13441 gcgtaggtgg ctaccgaaaa acccaatgtc aacacgtttg tagcttctgt taacccagcg
    13501 ggatgtaata ctatgtagat cagtccgcgt aggttcatca tgagttcggg ccagaccacg
    13561 tgaccgcgat taatcatcgg caaccacagc gtgactagtc ccattagacc gtcgcgacca
    13621 accatcacca gtgagagagc accaagcaac agtccgacag acataaagcc aagtacggag
    13681 gcccaacgac gatgaatcaa aagaatccca aacaacggta gaacgaattg cggtttatag
    13741 aatcctaacg ccaacagccc gccggcgatt gcatagcggt tgtacttaag gctcacaaaa
    13801 gtttcgacta gcagtagagt cgtgattaac gaatcctggc ccatctttag ggctgttatg
    13861 gttgggaaga atgcgagcac cataagaata aacaaactcc ggtgggtagt ttccataaaa
    13921 gaaaaaagtc ggaccaacgc tagcgcaagc agaaaaacgc taagtgccgt ccaaagcgca
    13981 tgggcaaccg gaaacggata ttctaccaga ggcagaagtg gcaatagttc gtagggagga
    14041 tggttgaacg gcaaatccca atctctgtcg tcgccgccga agcttttttg atatttcctt
    14101 tgcacctcca ggtcgaacaa ctctgcgccg tttccatcat tgataatctt ggcggcgctg
    14161 tagtacagca caaaatcgaa atagccggcc tgaatatcat ccagttgcgt ccagacaacc
    14221 gcgcactgac cgaacagaga gaataccaac aacggcacaa agaccagttt gaacagtcga
    14281 tgccgttgcg tcacaaggat tgtaggattg attggcgtgg ggatattcct tcgatgtcgt
    14341 agtgaagaaa acaacaattc attatacatc gctggacgga gtacgcagaa ataaaattcc
    14401 ggccgcggat tgttcgccgg cagtatgctg aggagatccg gatgataccg ctatgttcca
    14461 cgcgttcgct gctcgtgcga atcgttcctg cctgaaacat tcgtgataaa cggcaattga
    14521 ttgaggctta acagaagagc gtgctaagta atgatatggc cttggtgcga ttaaatgcga
    14581 cggctcttag ggggaatgac cgttcaaggc aacgcacggc acttggtatg cgttcgggct
    14641 tcatgttggg gctcctaaga ctaaggcgct tgcggctctc gtcaaaaatt ctacctttct
    14701 gctcaaacgg tcaattttag ggaagagcca atcgactcag catggcgcaa aagttttacg
    14761 tggtatggtc aggacggcag accggtgtat ttaccgattg ggcgaccacc caacgcgccg
    14821 tcgagggata cgccggtgcg cgctacaaat ctttctcatc ccgggcagaa gcagagcaag
    14881 ccttccatgc tggcgtccct gcaaacactc cgcctgagag atctagcggg caaagaccgc
    14941 gcgcatcggc gaaggaacga cgtgccgcgc atatcgctca ccggttccat atcactatct
    15001 attgcgatgg tgcctgtgaa cccaatcccg gccatgccgg ctccgggatc gtgatatatc
    15061 gagcgggcaa actcgccgaa ttgtggtacg ggctttataa tcccgtggga accaacaaca
    15121 ccgccgaact gaacgcgctc tatcatgcat tgcgtatggc cgaagcagag ctcgaggccg
    15181 gcaacagcgt cgaggtgtgc agcgattcag cttactccat caactgcatt cgcctttggg
    15241 cgcccggctg ggaaaagcga ggctggacca aaactggcgg cgcgatcaag aacctaaaaa
    15301 ttattcagga ctgctacgcc atctatcgcc ggataaaaga aaaactcacg ctcactcacg
    15361 tcgccgggca cgtcggcact gagggtaacg aactcgccga ccgcatggcc atgctcgctg
    15421 tccggagcaa acagacagag ctgcggctat atcacgaaac ctcagatatt ccgacgctgc
    15481 tgaagagatt ccgagcaaga cctgcacaac cgctaactaa ctagatttac ctagcgggcg
    15541 aatagcactt cgaacgaaaa tcccgccgtg taataactca ggccgactcc tgtcagagtt
    15601 aatgttaccg tgcgcctctc cttgtgatcc cattgcgctt gacattctgt agctttaaag
    15661 ctacattgat atgctatgg
//
LOCUS       DASUVS010000092         2651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_687284, whole genome shotgun sequence.
ACCESSION   DASUVS010000092 DASUVS010000000
VERSION     DASUVS010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687284"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..2324
                     /locus_tag="VF182_05180"
     CDS             75..2324
                     /locus_tag="VF182_05180"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6436502.1"
                     /translation="MAFRTIGKTLPRIEGEGKVTGQTKYAADLAFENLLWAKVLRASV
                     PHARIVNIDTSKAKALKGVRAVLTGVDVKDIYVGTRVKDQPVLAYDKVRMSGDAVAAV
                     AADSEEIAEEAIGLIDVQYEELPYVEDPVEALKPSAPLIHDDRDKYKNAPKLPEGVSP
                     HNLQSYVLWKNGDLEAGFQKAARVFEHTFRTPLSHHGYIEPCACTVQAHDDGRVEVWA
                     SNKGPWGLREQMAEDFGLEKENIKVHIVHVGGDFGAKASLIDVPVAYYLSKATKQPVK
                     LVFDYTDELLAGGHRHPAVLALRTGVDQDGHFTAIKATIHFSGGAYGSQKANPQVTVL
                     GGRRLASMYRVPAISCETYCSYTNQVPCTQTRTPGSPQVVFAFESQVDIIAKEMGIDA
                     LELRRRNILQDGDANPMGEKWTDILMGEVLERVVKTSGWKKNGAKKNRGWGMALYDRG
                     TPEGKASSALTLEADGRVNILTGVPDVGPGYYTVSQQMVCETLGLPPEKVGVVFKDTD
                     SLPFDPGTGGSKQTNTSGHAVNKSAQEVRERLVTLAARELGCQPEEIRQEGGKLTAPN
                     KKSTTTQKMIELAVRENGGPVFHLTNFVPKDMPKVTGFAAQVAEVEVDPNTGAVRVLN
                     LTAAHDTGTVLNHLTLTGQIEGGIITGLGFALMEENPMIDGKIASLTMGESKLPCIAD
                     VPPLKTVLVESPTGPTPFGGKAIAENPNVPTAAAIANAVADACGARIFDLPLSPEKVF
                     WALHGKINN"
BASE COUNT          628 a          704 c          787 g          532 t
ORIGIN      
        1 cgagttttcc gacaatcgaa aatccaaaag cgaccccgca gactaaaatc gaaaataata
       61 ttgggaggtg aatcttggca tttcgcacca taggaaagac cttgccccgc atcgaggggg
      121 aagggaaagt caccggacag acaaaatacg cagcggactt ggcgtttgaa aatctattgt
      181 gggctaaagt tttgcgcgct tcggtgccgc acgcgcggat cgtcaatatc gatacctcca
      241 aggcaaaggc tttgaaaggc gtgcgcgctg tgctgaccgg cgtggatgtc aaagacatct
      301 atgtcggcac gcgggtgaag gaccaaccgg tcttggctta cgataaagtt aggatgagcg
      361 gggacgcggt ggcggcagtt gcggccgaca gcgaggagat cgccgaagag gcgattggac
      421 tgatcgatgt tcaatatgaa gagcttcctt acgtcgaaga tccggtcgaa gcgttgaaac
      481 ctagtgctcc actgattcac gacgaccgtg acaaatataa aaacgcgccg aaattgccgg
      541 aaggtgtttc cccccacaac ttgcagtcct atgtcttgtg gaaaaacggc gacctcgaag
      601 ccggtttcca gaaggctgcg cgagtattcg agcatacttt ccgcacaccg ttatctcatc
      661 acggttatat cgagccctgt gcctgcacgg tccaggctca cgacgatggt cgggtcgaag
      721 tttgggcgtc gaacaaagga ccttgggggt tgcgcgaaca gatggccgaa gacttcggcc
      781 tggagaagga aaatattaaa gtccatatcg ttcacgtcgg cggtgacttc ggcgctaaag
      841 catcgttgat cgacgttccg gtggcttatt acctgtcgaa agcgaccaaa cagccggtaa
      901 agttggtttt cgactacacg gacgagcttc tcgccggcgg ccaccgccat cccgcggtgc
      961 ttgcgctacg aacgggagta gatcaggacg gccattttac cgcgatcaaa gcgactattc
     1021 actttagcgg cggcgcttac ggctcgcaga aagccaatcc tcaggtaacc gtgctcggcg
     1081 ggcgacgcct tgcgagcatg tatcgagtgc cggcgattag ctgcgagact tactgctcgt
     1141 acaccaatca ggttccttgc acccagacgc gcacgccggg cagtccgcag gtcgtgttcg
     1201 cgttcgagtc gcaggtggac atcattgcca aggaaatggg catcgatgct ctcgagttgc
     1261 gccgaaggaa tattctgcag gacggcgatg ccaatcccat gggtgaaaag tggaccgata
     1321 tcctcatggg cgaagtgctg gaacgcgtgg tgaaaacttc aggctggaag aagaatggag
     1381 cgaagaaaaa ccgcggctgg ggcatggcgc tctatgatcg gggcactccg gaagggaagg
     1441 cgagctcggc attgacgctt gaggccgatg gcagggtcaa tatcctcacc ggcgtgcccg
     1501 atgtcggtcc cggctattat acggtcagcc aacaaatggt ttgtgaaacg ctggggttgc
     1561 cgccggagaa agttggcgtt gttttcaaag acaccgatag cctccctttt gatcccggca
     1621 ccggcggcag caagcaaacg aatacttccg gtcatgcggt caataagtcc gcgcaggagg
     1681 tacgcgagag attggtcacc ctggcggcgc gggaattagg ttgtcagccg gaggagatac
     1741 ggcaggaggg cggcaagctc acggcaccca acaagaagtc gacgacaacg caaaagatga
     1801 ttgagctggc tgtcagagaa aacggcggcc ccgtctttca cctaaccaat tttgtcccca
     1861 aggacatgcc gaaggtgacc gggttcgccg cgcaagtggc tgaggtggaa gttgatccga
     1921 ataccggcgc agtgagggtt ctaaatttga ccgccgccca cgataccggc acggtgctca
     1981 atcatctcac gctgaccggg cagatcgaag gcggaatcat caccgggctt ggctttgctt
     2041 tgatggaaga gaacccgatg atcgatggca agatcgcctc gttgacaatg ggcgaatcca
     2101 agctgccatg cattgccgat gtgccgccgc tgaaaaccgt tctggtggaa agtccgacgg
     2161 gtccgacacc gttcggcggc aaagccattg ccgaaaatcc caacgtgccg accgcggcgg
     2221 ccatcgccaa cgcggtggcg gacgcctgcg gcgcgcggat ctttgatctg cctctatctc
     2281 cggaaaaggt cttttgggcc ctgcacggta agatcaacaa ctgagatcct agccgggtgg
     2341 tgatggaatc aagacgtgga gccagtagag tgaaactatt ggcctaactt ctaaggtaag
     2401 cgcgccctaa ctcgccagaa tgagagcgtc ggcgaaaacg ctaccagcca tcgagccttt
     2461 tcgtccaagc ttgctcatct atcgatcctg cccgtttcgg tgttcaacag caaagttact
     2521 tctcgcgcga acgtggcctc agacttatga acccggctct ctggcagctt ggtgacagat
     2581 tctttccgtt cgactatgca agccagattt atatcccgcc attagctcgg cgggcaggca
     2641 acagctagtc c
//
LOCUS       DASUVS010000093        10741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1343067, whole genome shotgun sequence.
ACCESSION   DASUVS010000093 DASUVS010000000
VERSION     DASUVS010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10741
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343067"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /gene="pgk"
                     /locus_tag="VF182_05185"
     CDS             <1..854
                     /gene="pgk"
                     /locus_tag="VF182_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX6436503.1"
                     /translation="ILLENLRFHAEEENNDPLFSRALASLADVYVNDAFGAAHRAHAS
                     TAGMAAFFEVKAAGLLLQRELNYLSRVLLTPEKPFIAILGGAKVSDKIGVIKNLLPKL
                     DALLIGGGMAYTFLKANGVNIGLSLVEQDKIPVAKELMADAIATGVKILLPTDHLTGD
                     ENKKNPSTSDINIAKGRMGLDVGPQTIRQFVAEIGKSKMVLWNGPVGLFEIEPFDQGS
                     KALAQALAARRSDIVSIIAGGDTVAAVTAAGVADDITHLSTGGGATLEFLEGRELPGI
                     KALEKSA"
     gene            851..1603
                     /gene="tpiA"
                     /locus_tag="VF182_05190"
     CDS             851..1603
                     /gene="tpiA"
                     /locus_tag="VF182_05190"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX6436504.1"
                     /translation="MNQPLVVGNWKMNGTLSQATRLAGTIVQGITQEKPNAALVLAPP
                     ATALAAVQERIAGSGISLAGQNCHWENNGAFTGEISPVMLSDLGCTYVIVGHSERRHI
                     FNESDEIIGRKLAAAIKNNLRPILCVGETLDQRTAGRTTEVIIRQMQLALKELNGNVI
                     ANLEVAYEPVWAIGTGHNADSKQIAEVHVAIRQYLTSSFDKTSLRILYGGSVNPENAN
                     ELGRIAELNGFLVGGASLKPETFLAIVRCFEQ"
     gene            1614..1994
                     /gene="secG"
                     /locus_tag="VF182_05195"
     CDS             1614..1994
                     /gene="secG"
                     /locus_tag="VF182_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX6436505.1"
                     /translation="MVIAVTIIHVIVCIGLILVVLLQTGKGAEIGAVFGGGSSSTIFG
                     SSGAGNFLTKLTTAMAIVFMITSLTLGYFAGQRPSATIFDSRSPASQPGAPAAPAKEE
                     AEATKSLDKSAAPSKHQPDKPANP"
     gene            2010..2096
                     /locus_tag="VF182_05200"
     tRNA            2010..2096
                     /locus_tag="VF182_05200"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2044..2046,aa:Leu,seq:gag)
     gene            2433..2636
                     /locus_tag="VF182_05205"
     CDS             2433..2636
                     /locus_tag="VF182_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6436506.1"
                     /translation="MKPADTAIKLLTLAEAATILKISKRTLHRMIQHRQIPAFKVGGQ
                     WRILESRFQEWVQEEESLAPKAS"
     gene            complement(2749..4212)
                     /locus_tag="VF182_05210"
     CDS             complement(2749..4212)
                     /locus_tag="VF182_05210"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6436507.1"
                     /translation="MDDLRDFLDVLDEFEEVQRIKVQVDWNLEMGAITRRCYDLGAPA
                     ALFENIKGYPQGFRALGAPLGASKKRGHSLFARTALALGLRPSASAKEIMQTYLQRRE
                     NLIKPVVVSTGPCKENIIKGGDVDLLKFPIPLIHAGDGGRYIGTWHTVITKDPDSSWV
                     NWGMYRLMVHDRNTLGCLFPFQQHIGQMYQKYEAMNRPMPVAVAIGGQPVIPIVSCVQ
                     IPPHVNEVDVAGGLQNAPIRLVRAETVDLNVPASAEIILEGEVLPHERMVEGPFGEYM
                     GYEAGKSSPKPIMKIHAITYRNDPILPFSNMGMPVHEGQTATALIKGAEIYSELRKLG
                     FPVKGVFCPPHAVGHMAVVATEVPFVNFARRVAHAVWATKPGLFTYYIVIVDADVDPT
                     NMDEVIHAMTTKCHPVNGIHQVPHNPGFPVLLPFLPLKERLIGDAAGVIFDCTWPKDW
                     PPESIPVKASFENLWPNQLQERVIKNWRLYGFDGARE"
     gene            4460..4843
                     /locus_tag="VF182_05215"
     CDS             4460..4843
                     /locus_tag="VF182_05215"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX6436508.1"
                     /translation="MADANRSNEAYLGPGTKINGKLFFDGPTTIEGDVEGEITGQANV
                     TIGQQATVKGKITAASIVVQGKVMADVQAEKKLDIQPPGSVVGDVTTQSLVIGDGAVL
                     EGHVSMRKEGRVLPLRQEPKKEGVS"
     gene            5323..5640
                     /gene="sugE"
                     /locus_tag="VF182_05220"
     CDS             5323..5640
                     /gene="sugE"
                     /locus_tag="VF182_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEX6436509.1"
                     /translation="MAWISLAIAGILEVFWAIGLKYTEGFSKFWPSLFTVFGMIASFY
                     FLAKALKVIPVGTGYAVWTGIGATGTAILGIVLFGESAAPARVACITVIVAGIIGLKL
                     TSP"
     gene            5734..6651
                     /locus_tag="VF182_05225"
     CDS             5734..6651
                     /locus_tag="VF182_05225"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6436510.1"
                     /translation="MIIDCHAHLMPQSWFDPKSPKSIFDLDVLFREQDEAGVNITVFG
                     NNWIRSPDGADSLRVVQEFNEHAAELTAKYPQRLLGLACSVPFENGKILKETEKAVRD
                     YKLKGIMINTSVNGEYLDSPRATPFFELASELDVPLFVHPPKFTIGNEKMEIFRLPEL
                     LGRPFDTTLALTRFIFTGGFERFPKLKMICAHVGGALPMLPGRYGVGYELRKDMSFGP
                     WEPDVMTQPPASYIGQLYVDTVSYHPAAVQCAIDTLGIDHVVFGSDSPPVPLPLKRAV
                     DTVHSLHISDADKQKILGGTAAKLLGLAF"
     gene            complement(6798..7844)
                     /locus_tag="VF182_05230"
     CDS             complement(6798..7844)
                     /locus_tag="VF182_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEX6436511.1"
                     /translation="MATKVIDLRSDTVTKPSAAMRQAMAEAEVGDDVFMEDPSVNRLQ
                     QRAAEIFGREAGLFVPSGSMGNLVCIMAQTQPGQEVICEAAGHIYNYEMAAMAAIAGV
                     LPRIVNANNGIMTWEQISQAIRPKAYFRPQTGLVSLENTHNMAGGTVYPTRLAHAICD
                     HAHEAGLKVHLDGARIFNAAVHIGQSVVEITKKFDSVQFCLSKGLGAPVGSMIVGSRE
                     FIERCRSIRKMLGGGMRQAGVIAAAGLIALEEGPKRLYIDHENAKILAQGLAAIPGIR
                     IDPSYVQTNIVIYDVRETKLSSPKFLEMIARHQILGVAVDAERIRMVTHLDVDRSDVE
                     VAAGSIADALRSRT"
     gene            complement(7896..8648)
                     /locus_tag="VF182_05235"
     CDS             complement(7896..8648)
                     /locus_tag="VF182_05235"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6436512.1"
                     /translation="MPGILSPEELIADLQNTLHQRHPKPHPIRQLLLSGRLTKEQLQG
                     WAKNQFHEFRNIHRFFGIRYQKCPVPELRRLLLENMVEEEGEDLFGGKYPSHRELWVQ
                     FAGGLGIPADEILNYEPLPAIRAALEMYVSLVQQSHWAVAIGTGLVFEGGGPKRMKEE
                     REAIERYYPWIPTTTLDFFRAHEYHDEGHGDMVTKVIKQHCMEERLQAEMREAVKQRA
                     DIMWLQNDSIYNVFVRPSLSPEIIDQIEGALS"
     gene            8774..9538
                     /locus_tag="VF182_05240"
     CDS             8774..9538
                     /locus_tag="VF182_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6436513.1"
                     /translation="MVSLSLRGVTKKFGRIPAVNQANLDVHSGEFLVVVGESGCGKTT
                     TLRLIAGLESPDAGTIFIAGAPVNDIPVGKRNVQMIFQNFALWPHMRVFDESRYSNLS
                     LPLKVRKWSHDKIGELLRPLARRVGIEESFFRRKPMELSGGQQQRVALGRAMATAPQI
                     LLMDEPLSNIDPPNRLRMRGEILKFHQENRLTTIYVTHNVADGIALADRIAVMRNGRF
                     EQVDTTENLFRTPASAYVADFFQAEQIVHKPRFSSS"
     gene            9535..10320
                     /locus_tag="VF182_05245"
     CDS             9535..10320
                     /locus_tag="VF182_05245"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6436514.1"
                     /translation="MRTRLFLLAPLLVLLLFGPAYSACRELDVARGFTRAAHAAGADA
                     VVEFFGHNFFQIITASGTRIITDPIAPGIYPTPDISPHAITVGREHPNHNYVEIAKGS
                     PIVLRGLGNYGAEWNKISTTVRDVLIYTVPVYQQQFGNALKGAAFVFDLGTLCIAHLG
                     DLAHKLTDDQVKAFGKVDIAMIPIGGTFTMPPDTARDVLQQLKPKVAIPMHYRESTAL
                     LDMFVKGFPNRYLTSYSHAFSKGALPSPTQIMVFTPWGMRGYR"
     gene            complement(10391..>10741)
                     /locus_tag="VF182_05250"
     CDS             complement(10391..>10741)
                     /locus_tag="VF182_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436515.1"
                     /translation="KTGLFPIMHVIVIKRAIYEKDPWVAQTLYKAFCEAKDLCMRELY
                     DTNILRVSLPWTSAEYEDTRNLMTADYWPYGLSANRVNLETLHGYLYEQGLIKQKLNL
                     DELFARETLEAFKI"
BASE COUNT         2554 a         2888 c         2728 g         2571 t
ORIGIN      
        1 ttattctact tgaaaatctt cgcttccatg ccgaggagga aaacaacgat ccgcttttct
       61 cccgcgccct tgcctcgttg gctgatgtct atgtcaatga tgcattcggt gccgcccacc
      121 gcgcgcatgc ttccacggcc ggcatggcgg cgtttttcga agtcaaagcc gccgggctgt
      181 tgctacaacg agagctcaac tacctctcgc gcgtcctgct cacccctgaa aagcctttca
      241 tcgcgattct gggcggtgcg aaagtttcag ataaaatcgg cgtgattaaa aatcttttac
      301 ccaaacttga cgcgctgctc ataggcggag gaatggccta cacgtttcta aaggcaaacg
      361 gagtaaatat cgggctatcg ctggttgaac aagataaaat tcctgttgca aaggagctga
      421 tggccgatgc catcgcgacc ggcgtcaaaa tactcttgcc gacagatcat ctaaccgggg
      481 atgaaaacaa aaagaatcct tccacgagtg atattaacat tgcaaaagga cgcatggggt
      541 tagatgttgg gccgcaaaca attcgtcaat tcgttgcgga gatcggcaag tccaaaatgg
      601 tgctctggaa tggtccggtc ggactatttg aaatcgagcc gttcgatcaa gggagtaaag
      661 ctctggccca agcattggcc gctcgtcggt ctgacatcgt cagtattata gccggcggcg
      721 atacggttgc cgcggttact gctgcgggag ttgctgatga tattactcac ctatcgaccg
      781 ggggcggggc aactttggag tttctcgaag gacgtgagct tcccggcatc aaggcacttg
      841 agaaaagcgc atgaatcagc cgttggttgt cggcaattgg aagatgaacg gaacattgtc
      901 gcaggcaacc agacttgccg gtacgatcgt tcagggcatc actcaagaaa aaccgaatgc
      961 tgctttggtt ttggcaccgc ctgcaacagc tttggccgcg gtacaagaaa ggatcgctgg
     1021 atctggaatt tcgcttgccg gccagaactg ccattgggaa aacaatggcg cgttcacagg
     1081 cgagatcagc ccggttatgc tatcggatct cggctgcaca tatgtcatcg tgggtcattc
     1141 ggagcgtcgc catatcttca acgagagcga tgaaatcatt ggtagaaagt tagcagctgc
     1201 gatcaaaaac aatcttcgtc cgatcctttg tgtcggtgaa acactcgatc agcgtactgc
     1261 cggtcgaacg acggaagtta tcatcaggca aatgcaactt gcattgaagg agcttaacgg
     1321 aaatgttata gcgaacctcg aagttgcgta cgagccagtg tgggctatcg gaaccggtca
     1381 taacgcagat tcaaagcaaa ttgctgaagt tcatgtggcg atccgccagt atctgacgag
     1441 ctcattcgat aaaactagct tgcgaatctt atacggcggt agcgtcaacc cggaaaatgc
     1501 gaacgagctc ggcagaattg cagaactgaa tggctttctc gtgggaggcg caagcttaaa
     1561 gccggaaacc tttcttgcga tagttcgttg ctttgaacaa tgagtaaaaa aatatggtga
     1621 tagcggttac tattatacac gttattgttt gcattggatt gatattggtt gtgttgcttc
     1681 aaaccggtaa aggagctgag attggtgctg tgttcggcgg cggctctagc tcgacgattt
     1741 ttggcagcag cggtgcagga aactttttaa ccaagcttac aacggcaatg gctattgttt
     1801 ttatgataac ttctctgacc ctcggatatt tcgccggaca acggccatca gcgacgattt
     1861 ttgatagtcg cagccccgcg tcccagccgg gcgctcctgc ggcgccggct aaggaagagg
     1921 ccgaagcaac aaagtccctc gacaaatccg cggcaccgtc aaagcaccaa ccggacaaac
     1981 cggcaaatcc ataatgtcag caaaccttcg cgaggatggc ggaatcggca gacgcgctag
     2041 tttgaggggc tagtgcccga aagggcgtag gggttcaact ccccttcctc gcaccattcc
     2101 aggcaattca gagcagctac ttgattggta tctgtctgaa cgtgtcgtat cctagaagaa
     2161 tgaccgccgt tgagtttgaa taagttgtta gaatctgcct cgtggtactc gatggatacg
     2221 gcaccatgac tgatctgaat tcaggattgc tcgtcaccct ttggctgcca tttttgtcgc
     2281 cctgagttcg acgctgtcga ttttttgaac agaggcaagt ttagtctgcc ctccagcaac
     2341 ccggtttcgc tcatgaatat agatgcttac cttgtcgcat cgtaatggca taagccttgc
     2401 tcgactgcga tcattcggga gcatctatga ttatgaaacc agccgacacc gcgattaagt
     2461 tactgacgct agctgaggcg gcaactattt taaagatttc taaaagaacg ctgcatcgga
     2521 tgattcagca ccgccagatc cccgccttca aagttggtgg ccagtggcga attctcgaga
     2581 gtcgcttcca ggagtgggtg caagaagaag agagcctcgc acctaaagca agttaactcg
     2641 ccagtagaca aaatccctct tccgtcaaaa gacccttcct agaatctccg gcacgaaggt
     2701 gctcttagaa tcagcgacta tggaacgact gcttggtttt tccccgcgtt actctcgtgc
     2761 tccatcgaag ccatacaatc gccaattttt gataactctc tcctgtaact gattcggcca
     2821 gagattttca aaactggctt tcaccggaat actctccggt ggccaatcct tgggccaagt
     2881 acaatcaaag atgacacccg cggcatcgcc aatcaggcgc tctttaagcg gcaaaaaggg
     2941 aagcagcact ggaaatcccg gattgtgcgg cacctgatga attccattta ccggatgaca
     3001 cttggttgtc attgcatgga taacttcatc catgttggtt ggatctacgt cagcgtcgac
     3061 gatgacaatg tagtacgtaa acagccccgg tttggtcgcc cagacggcat gcgctacccg
     3121 ccgcgcaaaa ttcacaaacg gcacttcagt tgccaccact gccatatgtc caaccgcatg
     3181 aggcgggcag aataccccct ttaccggaaa tcctagtttt ctgagctccg agtaaatttc
     3241 cgcccccttg atcagtgcgg ttgcggtctg gccttcatgc accggcatcc ccatgttcga
     3301 gaatggcaga atcggatcat tgcgataagt gattgcgtgg atcttcataa tcggcttcgg
     3361 tgaagatttc ccggcttcat agcccatgta ttcgccgaaa gggccttcga ccatacgctc
     3421 gtgtggcaat acttctccct cgaggatgat ctccgcgctg gccggaacgt ttagatcgac
     3481 ggtctcggct ctcaccagac gaatcggcgc attttgcaat ccgccagcaa catctacctc
     3541 gttcacgtga ggcggtatct ggacgcagct cacgatcggt ataacaggct gaccaccgat
     3601 cgctactgcc accggcattg gccgattcat cgcctcatat ttttgataca tttgaccaat
     3661 gtgttgttgg aacgggaaaa gacaccccaa cgtgtttcga tcatggacca tcaggcggta
     3721 cattccccag ttcacccatg aactatccgg atccttggtg atcaccgtgt gccacgttcc
     3781 gatgtaccgt ccgccatcgc cggcatgtat caacggaatg ggaaacttaa gaagatccac
     3841 gtcgccgcct ttaatgatat tttccttgca ggggcccgtg ctgaccacga cgggtttgat
     3901 caagttttct cgacgttgca aataagtttg catgatttct tttgctgacg cgcttgggcg
     3961 gaggccgagg gccaatgcag ttcgagcaaa taacgaatgc cctcgcttct tgctcgcacc
     4021 caagggagcg cccagcgcgc ggaatccctg cggatagcct ttgatattct caaacagtgc
     4081 cgccggcgcg ccaagatcat aacagcgtcg cgtaatggcg cccatttcga gattccaatc
     4141 gacttgtact ttgattcttt gaacctcctc gaactcgtcc aagacatcga gaaagtctct
     4201 gaggtcatcc attttattct tctctccttg cgaacgaccc atgcaacttc cgaccggggt
     4261 cagaagctcg gcaatgttgg taggaatttg cgcagatcga ttctttggtc agcaacgttt
     4321 tcaacccagt tatgtccagc ttaatccatt tagacataga tcattcttgg cgcaagctac
     4381 ggatcaacga caattcttcg atggacaaat cgggcaaggt catctatatt ctgaaggctt
     4441 atagcgaaag gggaaactca tggcagatgc gaatcgatca aacgaggcct accttggacc
     4501 gggcacgaag attaatggca aacttttttt tgatgggccc acgacaattg aaggcgatgt
     4561 ggaaggcgaa ataaccggcc aggccaacgt tacgatcggc caacaagcaa ctgtaaaggg
     4621 aaaaatcacg gcagcatcca tcgtggtcca gggcaaagtc atggcggatg tccaagccga
     4681 aaagaagttg gatattcagc cgccgggttc ggtcgtcggt gatgtaacga cacaaagcct
     4741 cgttatcggc gacggcgccg tccttgaagg tcacgtatcc atgagaaaag aaggccgtgt
     4801 tctgccgctt cgtcaagaac caaaaaaaga aggtgtatct taaagttctc cacctaccgc
     4861 tgacgtacga gcttgcatct caacgacatc aaccggaatc gagccttttt aaacagataa
     4921 caggaaactt cagagcggcg ggcttgtctt cgacaagccc gctaggagaa gggaatctga
     4981 attagcctca ccagcaattc acactctcag cccgcgacca tccaatcgag taaatcaata
     5041 accgcatctt cccagagtac cgcaaggagc gatctgagat tccgtgcatc gtgacaagcc
     5101 gaaccttgtc gcactctgca gctactcagc cttagtctcg tcctgccgca gtgaattttc
     5161 gggccctagc gttcacgcta gcacctagat gaaccgcgag ccaagcagat ccgcctgagc
     5221 cgcattcgga gcgattcgaa gttgtgtgcc tgccaaattt accatagatt ataatcgtct
     5281 cgaacatacg ggtttaccgc tgcgtcactg tgtaggagtt agatggcttg gatcagtctg
     5341 gcgatagccg ggattctgga agttttctgg gcgatcggcc ttaaatatac cgaaggcttc
     5401 agtaagtttt ggcccagtct ctttactgtc tttgggatga ttgccagttt ctactttctt
     5461 gccaaggcgc tgaaggtaat tcctgtcgga actggatatg ctgtctggac cggcatcggc
     5521 gccaccggca cggctatttt gggaatcgtt ctcttcggcg agtctgctgc tccggcgcgc
     5581 gtggcgtgca ttacagtgat tgttgccggc atcatcggcc taaaacttac gtcaccgtaa
     5641 tcgatcagcg gtagtccgcg actatctcgc atcatatgag aaagttcgac aagggtattt
     5701 gaatggtttc tgctcagaaa ggatccactg gttatgatta ttgattgtca tgcgcatctg
     5761 atgcctcaaa gttggttcga tcccaagtct ccaaagtcga ttttcgatct cgatgttctt
     5821 ttcagagagc aggacgaagc tggagtgaat atcacagttt ttggtaacaa ttggatacgc
     5881 tcacccgatg gcgccgattc gctgcgtgtc gtgcaagaat tcaacgagca tgccgccgag
     5941 ttgacggcga aatacccaca gcgccttctc ggtttagcct gttccgtccc atttgagaac
     6001 ggcaaaattc taaaggagac cgaaaaagcg gtccgcgact ataagctaaa aggaatcatg
     6061 atcaatacga gcgtcaatgg cgagtacctc gactcgccac gcgcgacgcc gttttttgaa
     6121 ctagccagcg agcttgacgt tccgcttttt gtgcatccgc caaagttcac catcggcaat
     6181 gaaaagatgg agatctttcg tcttccggag ctgctcggca gacctttcga taccacgctt
     6241 gctctcacac gattcatctt caccggcggg ttcgaacgct ttccgaagct gaagatgatc
     6301 tgcgcccacg taggcggtgc cctaccgatg ctgccgggcc gttatggggt cggctatgag
     6361 ttgcgcaaag acatgagctt cggtccgtgg gagcccgacg ttatgactca accaccggcg
     6421 agctatatcg gtcaactcta tgtagacacc gtctcctatc atccggctgc agtccaatgc
     6481 gcgatcgata ccctgggcat tgaccatgtg gttttcggta gcgattctcc gccggtgccg
     6541 ctcccactca agagagctgt tgacacggtg cactccctgc atatctcaga tgcggacaaa
     6601 caaaaaatcc tcggggggac cgccgcaaag ctgctcgggc tcgcgttcta gtgttccttc
     6661 cgatgttggt cgggacctac aacggcaacc aatcggaacc taaacggagc gagtctgtcg
     6721 caccggcaac tggtcgatca cacgagcgcc cataagcata ttcaggcaat ttgcctgttg
     6781 aaactagcca gctaatttta tgtgcgagaa cgtagagcgt cggcgatact gcccgccgcc
     6841 acctcaacat ccgaacgatc gacgtccaaa tgcgttacca tccgaatccg ctctgcgtca
     6901 accgctactc caagaatttg atggcgggca atcatttcga gaaacttcgg ggagcttagc
     6961 ttggtttccc tcacgtcgta gatgacgata ttggtttgaa catagctcgg atcaatgcgt
     7021 atgccgggga ttgcggcgag tccctgggca agtatcttcg cgttttcatg atcgatgtag
     7081 agccgcttgg gtccctcctc cagcgcaatt aaccctgctg cagcgatgac gcccgcctgg
     7141 cgcatgccgc cgccgagcat tttgcggatt gaacggcagc gttcgataaa ttcgcgtgaa
     7201 ccgacgatca tcgatcccac cggcgcgccc aatcccttgc tcaagcaaaa ctgcacggaa
     7261 tcgaatttct tggtaatctc cacaacactc tgaccgatgt gaaccgccgc gttgaagatc
     7321 cgcgcgccgt cgagatgaac ttttaggccc gcctcatgag cgtgatcgca gatggcgtgc
     7381 gccaaccgag tcggatagac cgtacccccg gccatattgt gggtattctc cagagacact
     7441 aagcctgttt gcggacgaaa ataagccttc ggtctaatcg cctgagatat ctgctcccaa
     7501 gtcataatcc cattgttggc gttgacaatc cgcgggagaa ccccggcaat ggcggccatg
     7561 gccgccattt cgtaattata gatatgtccg gccgcttcgc agatcacttc ctggccaggc
     7621 tgagtctgtg ccatgataca gaccaggttg cccatcgagc ctgacggaac aaacaatcca
     7681 gcttctcgtc caaatatttc cgcggcacgc tgctgcaaac ggttgacact cggatcttcc
     7741 atgaacacgt cgtcgccgac ttccgcctcg gccattgcct gacgcattgc cgctgacggt
     7801 ttggttaccg tgtcgctacg caagtcgatt actttagtag ccattccaaa acttggtggt
     7861 caactgatct ttatcactga gtagagttcg cgcctttagc tcaaggctcc ttcgatctgg
     7921 tcgatgattt ctggcgacag cgagggccgg acaaagacgt tgtagatgct gtcattctgc
     7981 agccacataa tgtcggccct ttgcttgacg gcttcacgca tttcagcttg cagtctctcc
     8041 tccatacagt gttgcttgat caccttggtt accatgtcgc catgaccttc atcgtggtat
     8101 tcgtgagcgc ggaagaaatc caaagtcgtc gttggaatcc agggatagta tctctcgatc
     8161 gcttcacgct cctctttcat gcgcttaggc ccgccccctt cgaagacaag gcccgttccg
     8221 atggccacgg cccaatgact ctgctgcacc agagagacat acatctccaa ggccgcgcgg
     8281 atcgccggca gaggctcgta gttcaagatt tcgtccgcgg gaatacccaa gccgccggca
     8341 aattgcaccc agagctctcg atggcttgga tatttgcctc caaacaaatc ttctccttct
     8401 tcttccacca tgttttccaa taacaggcgg cgcaattctg gaaccggaca cttctgatag
     8461 cgaatcccga aaaagcggtg aatattgcga aattcatgaa actgattttt cgcccagccc
     8521 tggagctgct ccttggttag ccgaccgctc aagagcagct ggcggattgg gtgcggtttg
     8581 ggatggcgct gatgtaacgt attttgaaga tccgcgatca attcttcagg tgaaagtatt
     8641 ccgggcatag gaaatctcct ccaaaaagta aaaatggcct tgccataaag gacgcatcat
     8701 aaccaactcg tttgtgggcc gccaccggct ttatcattgt tttttacgcg ctcctttact
     8761 agcatgaatc gccgtggtct ctttatcact acgtggcgtg acgaagaagt tcggtcggat
     8821 tccggccgta aaccaagcca acctggatgt ccacagcggt gagtttctcg tcgtcgtcgg
     8881 cgagagcggc tgcggcaaga cgaccacact tagactcatc gccggacttg aatcacccga
     8941 cgctggcacc atcttcatcg ctggcgcgcc ggtcaatgac atccccgtgg gcaagcgcaa
     9001 cgtgcagatg atcttccaaa atttcgcctt gtggccgcac atgagagttt ttgatgaaag
     9061 ccggtatagc aatttgtcgc taccgcttaa agtgcgtaaa tggtcccatg ataagattgg
     9121 cgagctgctc cgtcccttgg ctcggcgggt cggcatcgaa gaaagttttt tcagacgcaa
     9181 gccgatggag ctgtccggcg gacagcagca gcgggttgcc ttgggacgcg ccatggcgac
     9241 ggcgccgcag attcttctca tggatgaacc gctgagcaac atcgatcctc ccaaccggct
     9301 gcgcatgcgc ggcgaaatct tgaaatttca tcaggaaaac aggctgacaa cgatttacgt
     9361 gacgcataat gtagccgacg gcattgcgtt ggcggatcgg atcgcggtga tgagaaacgg
     9421 cagattcgaa caagtcgata ctacagagaa tctgtttcgc actcccgcca gcgcttatgt
     9481 cgcagatttt ttccaagccg aacagatcgt ccataaaccg agattctctt cttcatgaga
     9541 acacgattgt ttctgttggc gccgctgctc gtgctcttgt tattcggtcc ggcctattcc
     9601 gcctgtcgag agctcgacgt cgcccgcggc ttcacccgag cggctcacgc cgccggagcc
     9661 gatgcagtgg tcgagttctt cggccacaat ttctttcaga ttatcaccgc gagcgggacg
     9721 aggattatca ccgacccgat cgctcccggt atatacccga cgcccgacat cagccctcac
     9781 gcgatcacag tcggccgaga acatcccaat cataactacg tagagatcgc caaaggaagt
     9841 ccgatcgttc tgcgtggctt gggcaattat ggcgccgaat ggaataaaat cagtacgacc
     9901 gtgcgggacg ttctgattta cacggtgccg gtttaccagc agcaattcgg caacgctctc
     9961 aaaggggctg ctttcgtgtt cgatctcggc actctgtgta ttgctcattt aggcgatttg
    10021 gcgcacaaac tgaccgacga tcaagtcaag gctttcggca aagtggacat tgccatgatt
    10081 ccaatcggcg gcacctttac gatgccgccg gacacggcgc gcgacgttct ccagcaactc
    10141 aaacctaagg tggcaattcc gatgcactac cgtgaaagca ccgcgctctt agacatgttc
    10201 gtgaaaggtt tccccaatag gtatttgacc agttacagcc acgccttcag caaaggcgcg
    10261 ctaccgagcc caacgcagat catggtattt acaccatggg gaatgagagg ataccggtga
    10321 gctcgttgtg agtgcgagcg gagccaacag gatgatgtcg accgatccaa ccggaggtta
    10381 gaagaaactg ctaaatttta aacgcctcaa gcgtttcccg cgcaaacagc tcgtccaaat
    10441 tcaatttctg cttgatcagg ccctgctcat aaagataacc gtgcaaggtt tccaagttca
    10501 ctcgattcgc acttaatccg taaggccaat aatccgccgt catcaaattg cgagtatctt
    10561 catattccgc cgaagtccaa ggcaagctga cacgcaggat attggtgtca taaagctccc
    10621 gcatgcacag atccttagct tcacaaaaag ctttatagag tgtttgggcc acccagggat
    10681 ctttttcata gatcgcccgt ttgatgacga tgacatgcat gattggaaac aagccggttt
    10741 t
//
LOCUS       DASUVS010000094         5486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_468948, whole genome shotgun sequence.
ACCESSION   DASUVS010000094 DASUVS010000000
VERSION     DASUVS010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468948"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VF182_05255"
     CDS             <1..579
                     /locus_tag="VF182_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX6436516.1"
                     /translation="HVPVGKPVKVILASEDVVHSFYVPAFRIKMDVVPGRYTATWFEA
                     TREGEFHLFCAEYCGTQHSGMIGRVVAMKPVDYQRWLTSQASEEPIAVSGERLFQQLG
                     CESCHQTTANEKGPALTGLFGRRVQLESGESVLADEAYIRESILDPHNRIVAGYRPIM
                     PTYQGQISEDALHQIIVYLKTLGRAPEEKKPS"
     gene            576..2213
                     /gene="ctaD"
                     /locus_tag="VF182_05260"
     CDS             576..2213
                     /gene="ctaD"
                     /locus_tag="VF182_05260"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX6436517.1"
                     /translation="MSVVAERTVASQARPPRWHYLNVNYGVRSWLLTVDHKRIALLYL
                     VSITLFFFLGGAAAVMFRLELLTPRADLFEAETYNKLFTLHGLVMIFFFLIPSIPAVL
                     GNFLVPLMIGARDLAFPKLNLLSWYVFIIGGIFTLTAAIMGGVDTGWTFYTPYSSTYS
                     NSYVALTVTGAFITGFSSILTGLNFIVTIHKMRAPGMTWLRLPLFIWSHYATSLIMVL
                     GTPVLAISLVLVALERLFQLGIFDPRLGGDPVLFQHLFWFYSHPAVYIMILPAMGVVS
                     EIVPCFARKGIFGYTFIAFSSIAIAVLGFFVWGHHLFVSSQSVYAGLVFSFLSMMVAI
                     PSAIKVFNWTATLYKGSIVYRTPLFYALGFIGLFTIGGLTGIFVATLPIDVHVHDTYF
                     IVAHFHYIMVGGAIMGYLGGLHFWWPKITGRLYPEGWARFSALIIFVGFNLTFFPQFI
                     LGYLGMPRRYAAYPDEFQVLNVMSSAGASILGIGYLIPLIYFFWSMRYGPYAGANPWD
                     ARGLEWTTASPPPTDNFQYKPQVSQPPYAYEEEQKVG"
     gene            2206..2856
                     /locus_tag="VF182_05265"
     CDS             2206..2856
                     /locus_tag="VF182_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HEX6436518.1"
                     /translation="MAEIHGTASLAHQFDDPEQQVEASTLGMWTFLATEILFFGGMFV
                     AYIVYRMSYPQAFAAASHHLDIILGTINTGVLIGSSLTMVLAVYSAQMGRRRGQILFL
                     AATLLLGLIFLSIKGVEYQHKFHEHLVPGGGFQFSDADPRHAAIFFSLYFAMTGMHAF
                     HMVIGAGLIIWMALRAWRGEFSPAYYTPVEMLGLYWHFVDVVWIFLFPLLYLIGRH"
     gene            2856..3137
                     /locus_tag="VF182_05270"
     CDS             2856..3137
                     /locus_tag="VF182_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX6436519.1"
                     /translation="MDSHAAAAQRKTYFLVFGALLVLTLTTTGVAFLNLGPLNTPVAV
                     AIAMAKAALVMIYFMHLRHSTFLTRVFAGAGLLWLLHFIIFTMSDYLTR"
     gene            3299..4033
                     /locus_tag="VF182_05275"
     CDS             3299..4033
                     /locus_tag="VF182_05275"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX6436520.1"
                     /translation="MKLHEVKETSRLFSAVSYNVHQCVGIDRRRSPARIAQVLNQLDA
                     DVIGLQEVHSENNGTGESHQMQYLADATGRIAIAGPMIARANGEYGNVLLSRHPVINA
                     RRLDLSVPGREARGAIDVDIDTGNGVLRVIVSHLGLRVRERIYQTKRLLAGIAKEATT
                     PVIVLLDFNEWFPWGFPILRFNEEFGKAPSLRTFPARAPIFSLDRIWVKPQRALISVR
                     RCITPLTRIASDHLPLHATIDISRKE"
     gene            4292..4678
                     /locus_tag="VF182_05280"
     CDS             4292..4678
                     /locus_tag="VF182_05280"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6436521.1"
                     /translation="MAQRLLLTIMFLVAITTLACHRSGDSSHAVPELSGTTATVRSNA
                     DLENAVNAKLRADEQLRSAELGVSANAANSEITLSGSVESEALRSKAVNLAKGVDSGV
                     TVKDQIEVKPTGRAADNAFGSRDRHG"
     gene            4689..>5486
                     /locus_tag="VF182_05285"
     CDS             4689..>5486
                     /locus_tag="VF182_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012410003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6436522.1"
                     /translation="MRYAVVGLGHIAQVAVLPAFSHARQNSELVALVSDDPAKHEKLR
                     SKYKVQRTYSYDEYDQCLRSGAIDAVYIALPNNLHCDFAVRAAQAGIHVLCEKPMAVT
                     EEECENMISAAKQARVKLMVAYRLHFEKANLEAVEIAHSGKLGEPRFFSSVFAMQVKE
                     DNIRLKRELGGGTLYDIGVYCINAARSLFRAEPSEVHAFSVSGNDPRFREVDEMTGAV
                     LRFPDERLASFTCSFGAADVSAYELVGTEGNLRVEPAYEYIGELRHRL"
BASE COUNT         1084 a         1551 c         1519 g         1332 t
ORIGIN      
        1 catgttccgg tgggcaagcc ggtcaaggtt atcctggcct cggaggacgt cgtgcatagt
       61 ttttacgtgc cggcctttcg catcaaaatg gatgtcgttc ccggccgcta taccgcgact
      121 tggttcgaag ccacccgcga aggcgagttt cacctgttct gcgccgaata ctgcggcacg
      181 caacattccg gaatgatcgg cagagtggtg gcgatgaagc ctgtggacta ccagagatgg
      241 cttacctcgc aagccagtga ggaaccgatc gcggtcagcg gcgagcggtt atttcagcaa
      301 ctcgggtgtg aaagctgcca tcagaccaca gccaacgaga aaggcccggc gttgaccggg
      361 ctcttcggcc gtcgggtcca actcgaatcg ggagagtcag tgctggccga cgaggcatac
      421 atccgtgaat cgatcctcga ccctcacaac cggatcgtcg ccggataccg gccgatcatg
      481 ccgacctatc aaggccagat cagcgaagat gcgctgcatc aaattatcgt ctatctgaaa
      541 acgctcggca gagcgccgga ggaaaagaag ccatcatgag tgtggtagcc gaaagaaccg
      601 tcgcctcgca ggcgcgtccg ccgcgatggc attatctgaa cgtcaactac ggcgttcgtt
      661 cctggctatt gaccgtcgat cacaagcgca tcgcactgct gtatctggtt tcgatcactt
      721 tatttttttt cctggggggc gccgcggcgg tgatgtttcg cctggagttg ctcacgccgc
      781 gcgccgatct tttcgaagcc gagacctaca acaagctttt caccctccat ggattggtga
      841 tgattttctt ttttctgata ccgtcgatac ccgcggtgct ggggaatttc ctggtgccgt
      901 tgatgatcgg cgcccgcgat ctggccttcc ccaagttgaa cctcttgagt tggtacgtgt
      961 tcatcatcgg cggcatcttc acgttgaccg ccgccatcat gggcggggtc gataccggct
     1021 ggaccttcta cactccgtat agcagcacct attcgaacag ttacgtggcg ctaaccgtga
     1081 ccggagcttt cattaccgga ttctcgtcta tcctcaccgg tctgaacttc atcgtcacga
     1141 ttcacaaaat gcgagcgccg ggaatgacct ggctgcgctt gccgcttttc atttggtcgc
     1201 attatgccac cagcctgatt atggttctgg gcactccggt gctggcaatc agcctggttc
     1261 tggttgccct cgagcggctg tttcaattgg gcatcttcga tccgcggtta ggcggcgacc
     1321 cggtgctgtt ccagcatcta ttttggtttt attcccatcc cgcggtctac atcatgatcc
     1381 tgccggcgat gggcgtggtc agcgagatcg tcccgtgctt cgcgcgcaag gggattttcg
     1441 gctatacgtt tatagctttt tccagcattg cgattgccgt cctgggtttt tttgtctggg
     1501 gtcaccacct gttcgtcagc agccaatccg tttatgccgg cttggtgttt tcgttcctta
     1561 gcatgatggt ggcgattccg tcggcgatca aggtgttcaa ttggacggcg acactgtaca
     1621 aaggatcgat cgtttatcgc acgccgttgt tttacgcgct gggattcatc ggtctgttca
     1681 ccattggcgg gttgaccggg atattcgtcg ccactctgcc gatcgatgtg catgtgcatg
     1741 acacctattt catagtcgct cattttcact acatcatggt cggcggcgcc atcatgggat
     1801 accttggcgg gctccatttc tggtggccaa agattaccgg caggctttat ccggaaggat
     1861 gggcgcgttt ttcggccctg atcatattcg tcggctttaa tctgacgttt tttccgcaat
     1921 ttattttggg ttatctaggc atgccgcggc gctatgccgc ctatcccgac gagtttcaag
     1981 tgcttaatgt catgtcgtcg gcgggcgcgt cgatcctcgg cataggctat ctgattccgt
     2041 tgatttattt tttctggtcg atgcgctatg gcccgtatgc cggcgccaat ccctgggatg
     2101 ccagaggcct ggagtggacc acggcttcgc cgccgccaac cgataacttc cagtataaac
     2161 cgcaggtatc gcaaccccct tacgcttacg aggaggagca gaaagttggc tgagatacat
     2221 ggcacggcct cgctggcgca tcaattcgat gatcccgagc agcaggtgga agcgtcgact
     2281 ctcggcatgt ggacgtttct ggctaccgag atccttttct tcggcggcat gtttgtcgct
     2341 tacatcgtct atcgcatgtc ctacccgcag gcgttcgccg cggccagtca ccatctcgat
     2401 atcatcctcg gcacgatcaa caccggagtt ttgatcggca gcagcctgac aatggttcta
     2461 gcggtttaca gcgcgcagat ggggcggcgg cgcggtcaga tcctatttct tgcggcaacc
     2521 ttactgctag ggctgatttt cctcagcatc aaaggagtcg aataccagca caagttccat
     2581 gagcacctgg ttccgggcgg cggctttcaa tttagtgacg ccgatccgcg tcacgcggca
     2641 atctttttct cgctctattt cgctatgacc ggcatgcatg cttttcatat ggtgatcggc
     2701 gccgggctga ttatctggat ggcgctgcgc gcctggcgcg gagaattttc tccggcgtat
     2761 tatactccgg tagaaatgct gggcctctac tggcatttcg tagatgtcgt atggatattt
     2821 cttttcccgc tgctttacct gattggacgg cactgatgga ctcgcacgcc gcagccgcac
     2881 aaagaaaaac ttattttctg gtattcggcg cccttctggt ccttacctta accaccaccg
     2941 gcgtggcatt cctcaatctg gggccgctca acaccccggt cgcggtggct atcgccatgg
     3001 ccaaggcggc gctggtgatg atctacttca tgcatcttag acacagcacc tttctaaccc
     3061 gtgtgttcgc cggagccggc cttctctggt tgttgcattt tatcatcttc accatgagtg
     3121 attatctcac acgctgactg agcgccttgc catttccgag cctgctggta cgatttctcg
     3181 tcggtttgat tcggtcaaag gagttgcctt cgtccgattc cgacagtagt tacagtgaat
     3241 tatagtttga ttggtccaga ccggagggct ggctctaaac tgccggttcg atcaccgttt
     3301 gaaactgcac gaagtcaaag aaaccagcag gctattttct gcggtaagct acaacgttca
     3361 ccagtgcgtc ggtatcgatc ggcggcgcag cccggcgcga attgcccagg ttctcaacca
     3421 actcgatgcc gatgtcatcg gcctgcagga agtccactct gaaaacaacg gcacggggga
     3481 gtcgcaccag atgcagtatc tggctgacgc taccggaagg atcgccattg ccggaccgat
     3541 gatcgcgcgg gccaacggcg agtacggcaa cgtattgctg tcgcgtcatc cggtgataaa
     3601 cgcccgccgg ctcgacttga gcgttcccgg gcgagaagcg cgcggcgcca tcgatgtcga
     3661 tatcgatacc ggcaacggcg tgctgcgggt aattgtgtcc cacctcgggc tgcgagttcg
     3721 cgaacggatt tatcaaacca aacgcctttt ggccggcatc gctaaagagg ccacgacacc
     3781 ggtaatcgtt ctgctcgact tcaacgaatg gtttccctgg ggctttccca tcctgcgctt
     3841 caatgaagaa ttcggcaaag caccctcatt acggactttc cctgcgcgtg cgccaatttt
     3901 ctccctcgat agaatctggg ttaaacctca gcgagcgctg atcagcgtgc gccgctgcat
     3961 tacaccgctt actcgaatcg cttcggatca tctgccgttg catgccacta tcgatatcag
     4021 ccgaaaagaa tagcagatcc cggcgcgact ctgttcctgt tcttctgtcc gagcgttctc
     4081 ataaccaatt cctccttcac gatcaattcg ccgtcccagt tctgcatggg agcttcgcgg
     4141 actcgtgccg gctttgggac gagaaatcag cacggcagcc atgcgcgcaa ggagaacgcc
     4201 agcgcaaaag tccagtgcta aaacaccagc tgcgtggcat atttcttgcc cccaattgtc
     4261 atgaacaacg acagttccgg gaggcgtgct cgtggcccag cgcctactac tgacaatcat
     4321 gtttctagtg gcgatcacca cgcttgcctg ccacaggtcc ggtgattcct cccacgcagt
     4381 gccggagctc agcggaacca cagccaccgt gcgaagcaac gccgacctcg agaatgcggt
     4441 taacgcgaag ctcagagccg atgaacaact gcggtcggcg gaactggggg tgagcgccaa
     4501 tgcggccaat agtgagatta ctctatcagg atcagttgag tccgaggcat tgcgttccaa
     4561 agccgttaat ctggcgaaag gcgtagactc aggagtgacc gtgaaggatc aaatcgaagt
     4621 aaaaccgact ggccgcgccg ccgacaacgc tttcgggagc cgagatcgcc atgggtgaac
     4681 gatctagaat gagatacgct gttgtcggtc tggggcacat cgcgcaagtt gccgtgttgc
     4741 ctgcgttctc gcacgcccgc cagaattcgg agctagtagc tttggtatcg gatgaccccg
     4801 ccaagcatga gaagctgcgc agtaaatata aagtccaacg tacttactcg tatgatgagt
     4861 atgaccaatg tttgagaagc ggcgccatcg atgccgtgta tattgcgctg cctaacaatc
     4921 tgcactgtga ctttgccgtt cgcgcggcgc aagccgggat tcatgttctc tgtgaaaaac
     4981 cgatggcggt gaccgaggaa gaatgcgaga acatgatctc ggccgccaaa caggcgcgag
     5041 tgaagctgat ggttgcgtac cgtctgcatt tcgagaaggc caatctggaa gccgtcgaga
     5101 tcgcgcattc cggtaagctg ggcgagccgc gattcttttc gtcggtgttt gcgatgcaag
     5161 tcaaggaaga caacattcgc ctgaagcggg agctcggcgg cggcacgctc tacgatattg
     5221 gcgtgtactg catcaacgca gcgcgctcgc tttttcgtgc cgaaccgtcg gaagtccacg
     5281 ccttcagcgt tagcggcaat gatccgcgtt tcagggaagt cgatgagatg accggcgccg
     5341 tcctgcgttt tcccgacgag cgactggcat ctttcacctg cagtttcgga gctgccgatg
     5401 tttcggccta tgagctcgtc ggcaccgagg gcaaccttcg cgttgaaccc gcttatgaat
     5461 atattggaga gctcaggcac cggctt
//
LOCUS       DASUVS010000095         9246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1343792, whole genome shotgun sequence.
ACCESSION   DASUVS010000095 DASUVS010000000
VERSION     DASUVS010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9246
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343792"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            381..1199
                     /locus_tag="VF182_05290"
     CDS             381..1199
                     /locus_tag="VF182_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEX6436523.1"
                     /translation="MPIFMDRHDVRDATAEAVAAAHQEDLKIQANHACKSLTYWYDEQ
                     RGTAFCLIEAPTAAAVRALHREAHGLIPNVVIEVNPSTVSSFLGRVTDPDGAPGEPIR
                     EGGFRAIMFTDMANSTLITHSLGDAKAQSVLAKHHEIVRQALLAHEGREVDRAGDGFL
                     TCFASVSQAVACAVAIQRTFNAYNLSGAGLVDIQVRIGLGAGEPLTDGDTLFGSTVNL
                     TARICACAEPGQILAARVIPELCVGKSFSFKRYGEVNLKGFPESVELYEVDWMG"
     gene            1367..1768
                     /locus_tag="VF182_05295"
     CDS             1367..1768
                     /locus_tag="VF182_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX6436524.1"
                     /translation="MNEKVSQSEYLVISRGQWDRELSPQEIQKAIDQFYVWLERLVDE
                     GKMKTGQRLGNEGKTVSRKNVVTDGPYGEAKEVIGGYWFIIAAGLDEAAEIAAGNPCL
                     SCGLFFEIRPIDPVRASAFTVTTETPIENRR"
     gene            1881..2795
                     /locus_tag="VF182_05300"
     CDS             1881..2795
                     /locus_tag="VF182_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6436525.1"
                     /translation="MSQVLQGRVALVAGATRAAGRGIAVELGVAGATVYCTGRSTRAQ
                     ASELKRSETIEETAELVQQAGGQGIAVQVDHLEPAQVQALVARIEREQGRLDILVNDV
                     WGADFLIEWNVPVWKHSLKNGLRMLRLAIDTHIITSHFALPLLIKNPGGLVVEMTDGT
                     AEYNNTNYRLSLFYDLAKTSVMRIAWAQAQELRPHRCTAVAVTPGWLRSEQMLDNYGV
                     SEANWRDAIAKQPHFVITESPRYVGRAVAHLAADPDVERWSGQSVSSGQLAKVYGFTD
                     LDGSQPDAWRYVVEVQDAGKPADATGYR"
     gene            2887..3537
                     /locus_tag="VF182_05305"
     CDS             2887..3537
                     /locus_tag="VF182_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX6436526.1"
                     /translation="MARSPRITIPAYPHHIIQRGNNRSATSLPMMATRLFWNVFAKPK
                     SNVDAGLRVLMTNHFHLLVEPAEIGDLDRFMQSAGRRYVRYINETADRSRTLWDGRLK
                     TAPVSRDEYLSACSRYIELNPLRAGLVEHPKDYRWSSYQRCGLGVSDRLLDDDPWYTG
                     LGTTEQERQEEYRQWTDSQVNQANGTKSDRQRNAVAWSEKNLSKTDRRNDLTTISR"
     gene            3613..4845
                     /locus_tag="VF182_05310"
     CDS             3613..4845
                     /locus_tag="VF182_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX6436527.1"
                     /translation="MATKIFVFILAGALLCSRVAAHGQTQPTLPDGPGKEHVVAYCSG
                     CHGLSRVASSSYPQAYWNTVIRMMLNFGVPIAPDQITLVTDYLAKSFPEKPKPVANVI
                     AGPARIDIKEWQVPTPGSRPHDPLATRDGAIWYTGQMSNKLGRVDPKTGEVKEYPLKT
                     AQTGPHGLVEDKQGNIWFTGNHQALVGKLDPKTGAVTEYKMPDPKATDPHSLAFDQSG
                     ILWFTLQQSNMIGRLDPSSGEVKLVTAPTPRSRPYGIMVNSKGVPFVVLFGTNKIARI
                     DPKTMEIREYTLPDAKARPRRMAFTSDDVVWYSDYARGYLGRLDPATGQVTEWQSPGG
                     QRSEPYGIVAAKDIIWYSESGTKPNTVVRFDPKTQNFQTWVIPSGGYIVRKMDVTLDG
                     NPVLATSTVNGVAMVEVK"
     gene            5249..6412
                     /locus_tag="VF182_05315"
     CDS             5249..6412
                     /locus_tag="VF182_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEX6436528.1"
                     /translation="MFKDLFDKGKCALGFHTGEWRYLQDGSCEQTRICSRCRVASQQL
                     VHPWQTWDYESSNDCRMSRRCGRCAEAESKVEHVWASPIYASEQSCALVHPCSRCKET
                     SPAGNAHVWQSWSYDTANNCSQVLGCSRCGESGSERRTVHDWGAWQSSQFYDAPVRVC
                     RRCAEMVFNFDDQGEQNQVSLQMVDRAVWNVMEATDSAGVRERLSQNKKVLFDPVAEK
                     YFKFAIDQRAPDESARNTFRQLAGLIERCRSEGIEAVFAPAASQASAYAAASSSVGPG
                     RTPSSAPAGEKNARVVGHWRHTEARSSGGFSMAIDTHCVLDPSGRFEWWSKSMSSFGP
                     STSGPENGTWSASGNMLNLNFDDGTILAREVVIENTTMFWPGDGRYRLWERIS"
     gene            6432..8561
                     /locus_tag="VF182_05320"
     CDS             6432..8561
                     /locus_tag="VF182_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436529.1"
                     /translation="MAVKDWLDTGKCLLGFHQGDWRPESSVRCLLIQTCERCGTVNQR
                     LEHHWSDWNHADAQSCELSRACNRCAESERRIEHNWEAWTYRQDDSCDQSKRCQVCAE
                     WNDENRIEHQWGIWEYSERYRAPVHACGRCGLSASYFADQQIDQHVSSTNTNVRRKEL
                     RELISDDGAIEEMLARAEQCASSSEADVTPVAGSDDDISDSRQQMGLLRQMYQGLIAS
                     GQIASERQTFLTSILAELEEILGSAAPGIADKQIRVTRVQQLLSQMSEALLNPSRTKP
                     LEEPAGGSSRALLTELHANLHRYVMTETSAGLLTGEEGKALMALMGRLRGSREALADL
                     PSSDDSVKVETEVLRPLALEVRNFSLRHHLTLAQPVWPSRAVGQHGNALFYSGGSDVG
                     ALVAQVCASRKLRCLVPQADREPASLRWDQLRESAVAVFDFTGYKRVASLEEASPIAA
                     VAYELGIALALGRAAVVVATDGQDLPFDLDIEPICLKNDDSDVAMMSAAFDRALYGLQ
                     RGAAGSSVKSSIKYLRDRFAGHPSTHVRISLETLDDETMGDPIKARHLITSTFGFLGA
                     EALQILLPAWPGHYPDTSRRRCFHVTAFGPTWANNTRRLVGDACGSQIEYIRGDGVLA
                     PDILRSIWDEICRATHVVVDLTGLNANVVLELGIAHTLGRNVLLISQDQQVERCFRAI
                     AKHRIHPYTLEGSAAPALEAILQRFLT"
     gene            complement(8693..8833)
                     /locus_tag="VF182_05325"
     CDS             complement(8693..8833)
                     /locus_tag="VF182_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436530.1"
                     /translation="MLAFNARNRGSAIAGIWGSVLHFKKEKSRPLTLVVREINRLGLQ
                     IR"
BASE COUNT         2111 a         2489 c         2657 g         1989 t
ORIGIN      
        1 ccgcgtattg agcagtatac ccgcaaggac gttttacgaa cgcaacggct acgtttctaa
       61 tggcgctccg aggcatgtag gccggattct tggtgatttt ccgctgatca agaggatgcc
      121 acgtaaacat cgcccttgac ctgagattct acatgcgtga gggtcgcgcg gcagatatct
      181 tagaccctgg tcaagtgaaa aatagccact tacgtgtgac gaatttccgg tgataggaat
      241 ttcattgatc ctcggaaggt gcgttgcgac acgttttggc ggctcagcta agcttcaccc
      301 tagacgtgag ccgcttgtac agccacgccg cgcagcacag tctttagaaa aagaatagtg
      361 aggagtttgg agaattcgat atgccgatct tcatggaccg ccacgacgtt cgagacgcaa
      421 ccgcggaagc ggttgccgcc gcccaccagg aagatctcaa gatccaggcg aaccacgcgt
      481 gtaagtccct tacttactgg tatgacgagc agaggggaac ggcattttgc ctgatcgagg
      541 cgccgacagc agcggcagtg cgggcattgc atcgcgaagc tcacggtctc attccgaacg
      601 tcgtcatcga agtgaatccg tcgacggtct ccagctttct cggtcgagtt acagatcctg
      661 atggcgcacc gggcgaacca atccgagaag gaggctttag ggccatcatg ttcaccgaca
      721 tggcaaattc cacgctgatt acccactcgc tcggagacgc caaggcgcag agtgtgttag
      781 cgaagcacca tgagatcgtt cgacaggctt tgctggctca cgaggggcgc gaggtcgatc
      841 gcgccgggga tggatttctg acgtgctttg catcggtctc gcaggctgtg gcctgcgcgg
      901 tggcgattca gagaacgttc aacgcttaca atctcagcgg ggcaggtctc gtcgacattc
      961 aggtgcgtat cggtctcggc gctggcgagc ccttgaccga tggtgacacg ctgttcgggt
     1021 caactgtgaa tctaacagcg aggatctgcg catgtgccga accaggtcaa atcctggcgg
     1081 cgcgggtgat tcccgagctc tgtgtaggca agtctttcag tttcaaacgt tacggtgagg
     1141 ttaacctgaa aggctttccg gaatcggttg agctttacga agttgattgg atgggatagt
     1201 cggatcacgg ctattgctgg agcgaacatt tttattcgtc gctttggtga cgatgaatac
     1261 tttgtctatc gcctcgaccg tgcagactag atacgagctg cggagagctg ccgttaaaca
     1321 acctcaaact gctgatgcgg catgaaacca atgtggagga gacgtcatga acgaaaaagt
     1381 ttcacaatct gaatacctgg tcatttctcg cgggcaatgg gacagggaac tctcgccgca
     1441 ggaaatacag aaggcaatcg atcaattcta tgtctggttg gagcggctcg tcgatgaggg
     1501 aaaaatgaaa accggacaac gactcgggaa cgaggggaaa acggtctcaa gaaaaaatgt
     1561 cgtgacggac ggtccttatg gcgaagccaa agaagtaatc ggcggctact ggtttatcat
     1621 cgccgccggt ctcgacgaag cagccgaaat tgccgcagga aatccatgtc tgagctgcgg
     1681 cttgtttttc gagattcgcc ccatcgatcc ggtgcgggcc agcgctttca ccgtgacgac
     1741 cgaaacgccc attgaaaaca gaaggtgatt agaagatctc tttaaccgta ggcttgcctc
     1801 agtgtgaaat taacttgtgt ggctctcacc agtgacgatg ctatcggaaa tgtttctgta
     1861 actgatgaaa aaagtagatg atgagtcaag tactccaggg tagagtggcg ctggttgcag
     1921 gggcaacgcg cgccgcgggc cgcggcatcg ctgtcgaatt gggcgtggcg ggagccaccg
     1981 tgtactgcac tggccggagc actcgcgcgc aagcttcgga gctcaagcga tccgaaacca
     2041 ttgaagagac cgccgagctg gtgcagcaag ccggggggca gggcatcgcc gtgcaagtcg
     2101 atcatctcga gccggcgcaa gtgcaagcgc tcgtggcgcg gatcgagcgg gagcaaggcc
     2161 gcctggatat actggtcaac gatgtgtggg gcgcggactt tttgatcgag tggaacgtgc
     2221 cggtgtggaa acattcgttg aaaaacggcc tgcgcatgct gcggctcgct atcgacacgc
     2281 acatcatcac cagccacttt gcactgcccc tgttgatcaa gaatccgggc gggctggtcg
     2341 tcgagatgac cgacggcact gccgaataca acaatacaaa ttaccggttg tctctgtttt
     2401 atgatctcgc caagacttct gtcatgcgca tcgcctgggc acaggcgcaa gagcttcggc
     2461 cgcaccggtg cactgcagtt gccgtcacac cgggctggct gcgttccgag cagatgctgg
     2521 ataattacgg tgtcagcgaa gcaaactggc gtgatgccat cgccaagcaa ccgcatttcg
     2581 tcatcaccga atcgccgcga tacgttggtc gcgcggttgc ccaccttgct gcagatcccg
     2641 atgttgagcg ctggagtggt cagtcggtct cgagcggcca gctcgccaag gtctatggct
     2701 tcaccgacct cgacggttcg cagcccgatg cttggcgcta cgttgttgag gtccaggatg
     2761 ccggcaagcc agccgatgcc accggataca gataggtgga aaatcgattc agaaaacttt
     2821 ttttcttgcc gatgcagtaa tccggcgata ggctgtgcca cgaatctgcc gacacagaga
     2881 taacgaatgg cacgatcgcc gcggatcaca attcctgctt atcctcatca tattattcag
     2941 cgcggaaata atcgctctgc cacttctttg ccgatgatgg ctacccggct tttttggaat
     3001 gtcttcgcca agccaaaatc aaatgtcgat gcaggattac gcgtgttgat gaccaatcat
     3061 tttcatctgt tggtggaacc ggcggaaatc ggcgatctcg atcgattcat gcagagcgcg
     3121 gggcgaagat atgttcgcta tatcaacgag accgccgatc gatcacgcac gttgtgggac
     3181 gggagattga aaactgcccc cgtaagtcga gatgaatact tgagtgcgtg cagccgatat
     3241 atcgagctaa atcctctgag agcaggttta gtagagcatc caaaggatta tcgttggagt
     3301 agttaccagc gctgcggatt gggcgtgtcg gatcgtttac tggacgacga cccgtggtac
     3361 acaggtttgg gaacgactga acaagagaga caagaagaat atcgacagtg gacagactca
     3421 caggttaatc aagcgaatgg gacgaaatcc gacaggcaac gcaacgcggt tgcttggtcg
     3481 gaaaagaacc tttcaaaaac agatcgaaga aatgatttaa cgacgattag tcggtgaagc
     3541 tcgggatcga cctaggaaac gggatgtaac ctggatcgaa aaagttctct gaccccattc
     3601 tgtggaggaa cgatggcgac aaaaatattt gtgtttatcc tcgccggtgc tttgctgtgc
     3661 tcgagggtgg ccgcccatgg tcagacgcag cccactcttc ccgacggccc gggcaaagaa
     3721 catgtcgtgg cttactgcag cggttgtcac gggcttagcc gagtcgcgag ctcaagttat
     3781 cctcaagcct actggaacac tgtcatcaga atgatgctca atttcggtgt gccgattgcg
     3841 cccgatcaaa tcactctcgt cactgattat ctggcgaaaa gctttccgga aaaaccgaaa
     3901 cctgtcgcaa acgttatcgc cgggccggcg cgcatcgata tcaaggagtg gcaggttccg
     3961 acaccgggtt cgcggccgca cgacccgctt gccacccggg atggcgccat ctggtacacc
     4021 ggccaaatga gcaataagct cggacgggtc gatccgaaaa caggggaggt gaaggaatac
     4081 ccgcttaaga ccgcgcagac cggtccccac ggtctggtag aggataaaca gggcaatatc
     4141 tggtttacag ggaaccatca ggcgctcgtc ggcaaactcg atccgaagac gggcgccgtg
     4201 acggaataca aaatgcctga cccgaaagca acggacccgc actcgctggc gttcgatcaa
     4261 agcgggattt tgtggttcac gctgcagcaa tccaacatga tcggacggct tgatccgagc
     4321 agcggcgaag ttaagcttgt caccgcgccg acgccgcgtt cccgtcccta cggcatcatg
     4381 gtgaattcaa agggcgttcc ttttgtcgtc ctgttcggca ccaacaagat agcgcgcatc
     4441 gatcccaaga cgatggaaat tcgcgaatac accttgcctg acgccaaggc gcgtccacgt
     4501 cgtatggcct tcaccagcga tgatgtcgtc tggtattcgg attatgcgcg gggttatctc
     4561 ggccggctcg atcccgcgac aggccaggtg actgagtggc aatcgcccgg cgggcagcga
     4621 tctgagcctt acggcatcgt cgccgcgaaa gacatcattt ggtacagcga atccggcacc
     4681 aaacccaaca cggtcgtgcg cttcgatccc aagactcaga atttccaaac ctgggtgatc
     4741 ccctcgggcg gctacatcgt gcgcaagatg gatgttactc tcgatggcaa cccggtgctc
     4801 gctaccagca cggtgaacgg agttgcgatg gtcgaagtca aataaggaga aaagaccaga
     4861 tcggcgttga gccagagaac atccggatcg gatcacgctg catctcacgc agcgctggta
     4921 aaatcctgat tcaagcgttc aaccgctttg ctccgttcaa aaatgttcaa gactcatgat
     4981 cgactctcct cgtcctaggc aacagctctg cccattgcgc gacgcggaca aaccgcctcc
     5041 gcgattacct ccgtaccatt gcttcatcga tgatggtgaa tgtattggaa gctggtgaag
     5101 gagaccccca accgtgcaga cgattgttgc tggccggcca atcgcggatc aatccccgtt
     5161 gaagtggata aacctcaaac cctcgcctca gaaaaaaatt gacattttgc cgtaacaagc
     5221 ggagactccc gtcgagattt ttctttgtat gttcaaagat ctattcgaca aaggaaagtg
     5281 cgccctcggg tttcataccg gtgagtggcg ctatctacaa gatggatcat gcgagcagac
     5341 gcgtatctgc tcgcgctgca gagttgcaag ccagcagcta gttcatccct ggcaaacctg
     5401 ggactatgaa agctccaacg actgccgcat gtcgcgacga tgcggccgct gcgcagaagc
     5461 cgaaagcaag gtcgaacatg tctgggcctc acctatctat gcatcggagc aatcctgcgc
     5521 gctggttcac ccgtgctcgc gctgcaaaga gacttcaccc gccggcaatg ctcacgtctg
     5581 gcaatcctgg agctatgata cggcaaataa ttgctcgcaa gtcctcggct gctcgcgctg
     5641 cggcgagagt ggatcggaac gacgcacggt tcacgattgg ggcgcctggc aatccagtca
     5701 attctatgac gcgccggtgc gcgtttgtcg ccgctgtgcg gagatggtct ttaatttcga
     5761 tgatcaaggc gaacagaacc aggtttcgct gcaaatggtt gatcgggccg tctggaacgt
     5821 catggaagcg accgatagcg ccggcgtgcg cgagcgtttg tcacagaaca agaaagtgct
     5881 gttcgacccg gtcgccgaga agtattttaa gttcgccatc gaccaacggg cgccggatga
     5941 aagtgcgaga aacacttttc ggcagttggc cggtttgatc gagcgatgcc gcagcgaagg
     6001 catcgaggcg gttttcgcgc ctgccgcatc acaggcttcc gcttatgctg cggcatcctc
     6061 gagcgtcggt ccgggacgta cgccatcgtc agcgccggcc ggagaaaaga acgcgcgagt
     6121 agttggccac tggcgtcata ccgaagcccg gtcatcaggc ggatttagca tggcgatcga
     6181 cacccactgc gttttagatc cgagtggtcg atttgaatgg tggagcaagt cgatgagcag
     6241 tttcgggccc agcacatctg gacccgaaaa tggcacctgg tcggcctcgg gcaatatgct
     6301 gaacctcaac tttgacgatg ggactattct cgcgcgcgaa gtcgtcatcg aaaataccac
     6361 tatgttctgg ccgggcgatg ggcgctaccg actatgggaa cgaatttcgt gaattcgact
     6421 gcggcaaact tatggcagtg aaggattggc tcgataccgg aaagtgcttg ctcggttttc
     6481 atcaaggaga ttggcggcct gaatcctcgg tgcgctgcct cttgattcaa acttgcgagc
     6541 gttgcggtac cgtcaatcaa cgactagagc accattggtc ggattggaat cacgccgacg
     6601 cacaatcttg cgagctctcg cgcgcctgca accgttgcgc tgagtcggaa aggcggattg
     6661 aacataactg ggaggcctgg acgtatcgac aggatgattc ttgcgatcag agcaaacgct
     6721 gtcaggtttg cgctgagtgg aatgatgaaa accggatcga gcatcaatgg ggaatctggg
     6781 agtatagcga gcgttatcgc gcgccggtgc acgcctgcgg ccgctgtgga ttgagcgcct
     6841 cttactttgc cgatcagcag attgatcagc acgtctcctc aaccaacact aatgtgagga
     6901 ggaaggagct cagggagtta atttcggatg acggagctat cgaagaaatg cttgccaggg
     6961 ccgaacaatg cgccagtagt tccgaagccg atgtaactcc tgtagccggg tcggacgatg
     7021 acatctccga ctcgcgccag cagatgggcc ttctccgcca gatgtatcag ggtctcattg
     7081 ccagcggcca aattgcttcg gagcgccaaa cttttttgac atcgattctt gcagagctcg
     7141 aggagattct tggcagcgcc gcgccaggga tcgctgacaa acagatcaga gtaacccgtg
     7201 tccagcagtt gctttcgcaa atgtccgaag cgctgctcaa tccgagcaga accaaaccgt
     7261 tggaagaacc tgctggcggc tcgagccgcg ctctgcttac ggaactacac gcgaatctcc
     7321 atcgctatgt catgaccgag acctcggcgg gtcttttaac cggcgaagaa ggtaaagcct
     7381 tgatggcgtt gatgggacgg ctccggggca gtcgcgaggc gctcgcagat ctaccgagca
     7441 gtgatgactc ggtgaaggta gaaaccgaag tactgcgacc gctggccctg gaggttcgga
     7501 acttctccct acgccatcac ctgaccctag cccaacccgt gtggccctcg cgagcggtcg
     7561 ggcaacatgg caatgcgttg ttctattcag gcggttcaga tgtcggcgct ttggtcgcgc
     7621 aggtttgcgc cagccgcaag cttcgctgtc tcgtgccgca agccgaccga gaaccagcat
     7681 cgctgcgctg ggatcagttg cgcgaatcag ccgtggcggt gtttgatttt accggctaca
     7741 aacgtgttgc ctctttggaa gaagcgagcc cgattgcggc agtagcctac gaactcggta
     7801 tcgccctcgc gcttggacgc gccgcggtgg tcgtcgcaac cgatggtcaa gatctgcctt
     7861 tcgatctcga catcgagccg atatgtctta agaacgatga tagcgatgtt gcaatgatgt
     7921 ctgccgcctt cgatcgggcg ctgtatggct tgcagagagg cgcagccggc agctctgtga
     7981 aatcatcgat aaagtatttg cgcgatcgat ttgccggtca tccgagtact cacgtgcgca
     8041 tttcgcttga gacgctcgat gacgagacca tgggcgatcc gatcaaagcg agacatctga
     8101 tcacctcgac gttcggcttt ctcggcgccg aagctttgca gatcctgctg ccggcctggc
     8161 cgggtcacta tccggacacc tctcgcaggc gttgctttca cgtcaccgcc ttcggtccga
     8221 catgggcgaa caacacgaga cggctggtcg gagatgcttg cggttcgcag attgaatata
     8281 tccgcggcga tggcgtcttg gctccggaca ttctgcgatc tatctgggat gaaatttgcc
     8341 gtgctaccca tgtcgtggtt gatctcaccg gactcaatgc caatgtcgtg ctcgaactcg
     8401 gaatagcgca tacgctcgga cgcaacgttt tgctgatctc tcaagatcaa caagtcgagc
     8461 gctgtttccg cgccatcgca aaacaccgca tacacccata cacgctcgaa ggatccgctg
     8521 ccccggcctt agaggcaatc ttacaaagat ttcttactta gcgaaaatta gcttcgtgac
     8581 tgttaattca aaggagtgat ctacgcgatt tctagtgagg gtaaacatcc cggtcgagcc
     8641 cggaaatcag gccgcaagaa ccggcaagct cggcgcaacc atccagtcca tactagcgga
     8701 tttgaagccc gaggcggttt atttcacgga cgacaagggt caacggacgg cttttctctt
     8761 tcttgaaatg aaggacgctt ccccagatcc cggcaattgc cgagccgcgg ttcctggcgt
     8821 tgaatgcgag catcgagatt catccggtaa tgataacgga ggatctcgcc agggccagtg
     8881 gctcgatcga agcggccgcc aagaggtaca gctgatagcg cctgtgcaat ctatatcgaa
     8941 tgggccgcct tgctaccgcc gcttaggtct gcgcaccggc acccatggat aaggtaggaa
     9001 attgcctacc tcgcagatga tgcggatcag gtgattgctt cagccggcgc tgagcacgca
     9061 accttacaga gaactctcca atcacttgct ctcggtatat tttcgaatgg tatccttcac
     9121 ctcgggcggg gcttgaacca gctttcttgt caccctgtcc gcctcgccgc cgacttggaa
     9181 cgccggctca gagcgggtaa cccttttgaa ctcttggatg aattccggat ccttgatcat
     9241 ctgcga
//
LOCUS       DASUVS010000096         7536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_32389, whole genome shotgun sequence.
ACCESSION   DASUVS010000096 DASUVS010000000
VERSION     DASUVS010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32389"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(536..1519)
                     /locus_tag="VF182_05330"
     CDS             complement(536..1519)
                     /locus_tag="VF182_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436531.1"
                     /translation="MLTKCSVIVTIVLLLLHAPPCGSQERIKLRVSSATKTLGYGPLW
                     IASKMGFFERQGLDVDLVVIRASDVGIQALAGGSLEIAGSSADAPIAAVEKGLDLVMI
                     GGIINGLTQSIMAAKKYKTYADLRGATFGAISLTSGVTFALRQVLKVKGLEYPRDYKL
                     LVIGGTPQTYAALTAGQIDAAALSLPVNYAAEEQGFNEIGKFVDVIPNYQLAALSLKR
                     SWGEKNRPVVIRFSKAMAQTMRWIYHNKQPAIDFLAKEMNLKPDHARRGWEFYTTSGM
                     WHPDGDIILEGLQVVTQMYAEQTQTKSPAPNALKYVDQSYLREALKELGTR"
     gene            complement(1555..2769)
                     /locus_tag="VF182_05335"
     CDS             complement(1555..2769)
                     /locus_tag="VF182_05335"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6436532.1"
                     /translation="MLSANRILEGVRILTFTTGYAGPYAGRLLASYGAEVIKIESRKG
                     GLDTFRHYGQHQDIDAAPRFIECNLGVRSLSVNLKHSIGQRLIKELAGKADAILQNFR
                     PRVLDKLGLGDEELRKANPKLAILKMPGFGSDGPKSSYGTWGFNLTAFSGMTYLWNHP
                     EQNRPIGSQGVYPDHLGFIIGPTLMIAALLNSRRTGRGVTIDLAQIEGTAYTLGTTYL
                     EASVNGDDPKPQGNQYGGAAPHGCYRCQGDDRWCVISVRDDDEWRALCRVIGKAELAG
                     DPRFADCQARLKHSSELDQIVQRWTQTRTAEDVMTKLQSAGVAAGVVQTGADLLKDPQ
                     LRHRNYFAAFPDSLIGPFEIPRSGILFGGLEEEPYRLPNRFGSDNDQILGEVLGYDEA
                     TIERWRGDDVLT"
     gene            complement(2875..4083)
                     /locus_tag="VF182_05340"
     CDS             complement(2875..4083)
                     /locus_tag="VF182_05340"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6436533.1"
                     /translation="MINTTESAALLPGYRVLDLTSSMGALCGKLLGDMGMDVIKIEPP
                     QGDSARSEPPFAKGHAHREGSLRFAYLNAGKRSITLDITKSQGKRLLLRLTKSADVVI
                     ETFAPGFLASHNLGYSDLIEMQNKLILASLTGFGQDGPYAQFKAPDIVGTAMGGLLYI
                     SGDPKMAPCNPPETQSYYYASLFAAYGITLALWQRETRGIGAYIDASVQASLALHEHV
                     AFNYSAEGRVMKRAGSQHQHNAPANLFECKNGYIALFVTQAHWPLLLKVWGDHAPELD
                     DPKWINSNLRRANADYINAEVTSFTSRFLKEDLAELMQKHGIPGLPVNTPSDFMKDPH
                     IRARGFFATVTHPVIGSFEQPGSPFMVNGQRAAPAPAPLLGQHNQEIFCGELGLELNE
                     LAVLAADGVI"
     gene            complement(4233..5396)
                     /locus_tag="VF182_05345"
     CDS             complement(4233..5396)
                     /locus_tag="VF182_05345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6436534.1"
                     /translation="MDFNLPEELQLLKETVRKFVDKELIPLERECRPEGEDMPEQYIK
                     PLQEKAKAIGLWLLDVPPEYGGAGLDLMSRCVIMEEVARTVAIPFRYAPIFGPEVRPV
                     LFHCNEEQKERFLYPVIRGEKRVCFAQTEPDAGSDPAGMKTRAVKNGDHYIINGTKRF
                     ITGAKSADYAQVIAVTDPEKGARGGVTCFIVDMKAPGVTLERLWPTMMGDTPGQILFE
                     DVRVPAANIIGGEGQGFAIAQGWIQEGRIRGHGARPVGIASRALDMMIEYSKVRTTFG
                     RPLADRQAIQFMIADSAMELHACRLMVYECAWRHDRGEDVRHLSYMTKIMCAEMASRV
                     VDRSIQVHGGLGLMRDLPLEWWYRQVRSIRITEGTPEVLRWRLAQHIIKNYKS"
     gene            complement(5490..6323)
                     /locus_tag="VF182_05350"
     CDS             complement(5490..6323)
                     /locus_tag="VF182_05350"
                     /EC_number="4.1.2.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005756117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxycinnamoyl CoA hydratase/lyase"
                     /protein_id="HEX6436535.1"
                     /translation="MAEVKDFKTVKVEKDQGIAWVILNRPEKRNAMNPQLHYDMLEAL
                     TELEVDKDSKVVVITGAGPSWCAGQDLREYFRGTDTNPVERRKASWASQEWRWRRLFT
                     FPKPTIAMVNGFCFGGAFTPLIACDFAIAAEDAVFGLSEINWGIFPGGLVSRVLADAM
                     CYRDAMWYIMTGDPFDGKKAAELKLINYAVPKEQLRDETVKLAKKLMEKNPQVLRAAK
                     EVFKYCRTMDYNQAEEYMSAKGTALRLTDPEKGRETGMKQFLDEKTYRPGLGAYNREA
                     K"
     gene            complement(6403..7398)
                     /locus_tag="VF182_05355"
     CDS             complement(6403..7398)
                     /locus_tag="VF182_05355"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436536.1"
                     /translation="MNRKTFVWAITVFFLCVGWRAMAQDKVRMGLSSVSGLHTAVWVA
                     ETKGLFRKHGIDPEVIVTGQGGTAGISALIANDVQMVNSAGDVLVAASLRGGDTVMVA
                     SVVNKGLQRLITKPDIKTPAELKGKRVGVTRIGAVSHSVLLMMLQRWKMSVNDVQVLQ
                     LGSSPSMLASLDKGGIEGAVVTIPSMFVAEDRGYRVLLDMADTDIYYLHTMVATTRQY
                     IKNNRDKVVRFLKGYVEGIAFVKHNKQESLDIVKKKLRIGPEQEKNLERSIDLLIAKY
                     YESVPYPSLRGVETVLGFIEKDNPKAKGADPKMFVDDSLLREIEASGFVKTLYQR"
BASE COUNT         1626 a         2157 c         1978 g         1775 t
ORIGIN      
        1 tttgggaaat tgcgtaatct tgacgagtga agcacgatgg acagcgcaac cttggataca
       61 ctgtgtatcc gaagttgtta cgagtggact ggaaagagac cggctcacag agctcgtacc
      121 gcaaagatac ctgctttcca cattggttca ccgatctatc tgctaatgac aagattacag
      181 gtggtggcgt ggcggcttgg gagatacttt gtcctggcat gaacaaatgt gccggcaagt
      241 ttatagcgga gtgataattc ggtgtgagtt agttggccga agccaaactc cgggcccgca
      301 ggcatgttct ccctaatata ttggattgcc cggcatgtac ttgccgaggt agttcgccat
      361 cagcgggcgt aaaaatcgta gccgactcga tagccgacta aaatacgcaa gtgttttctc
      421 cctctggtaa ccgccttact gctgcggctg catggccaga aaaggaatcg acacgatcaa
      481 gcaagcaata gcggtggagt gaatttgtcc agcctgttct tacgcttggc accggctatc
      541 gcgtaccaag ctctttgagc gcctctctaa gataactctg atcaacgtat ttgagggcgt
      601 tcggcgcggg acttttggtt tgggtttgct cggcgtacat ttgggtcacc acctgcaggc
      661 cttccaagat gatatcgccg tcgggatgcc acataccgct ggtcgtgtaa aactcccagc
      721 cgcgccgggc gtgatcaggt ttaagattca tctctttggc aagaaaatct atggctggtt
      781 gtttgttgtg ataaatccag cgcatggtct gagccatggc cttcgagaag cgaatcacaa
      841 ccggccggtt tttctctccc caggatcgct tcagagaaag cgccgcaagc tgatagttcg
      901 ggattacatc gacgaattta ccgatctcat tgaagccctg ctcttccgcg gcgtaattga
      961 ccggcagcga cagcgccgct gcgtcgatct gaccggcggt caaagccgcg tacgtctgtg
     1021 gcgttccgcc gatgacgaga agcttgtaat cgcgcgggta ctccagccct ttgactttca
     1081 gcacctgcct gagcgcaaac gtcacgccgg aagtcaaact tatggctccg aaggtcgcgc
     1141 cacgcaaatc ggcgtaggtt ttgtacttct tggcggccat aatcgattgg gtaagtccgt
     1201 tgatgatgcc gccgatcatc accagatcaa gtcccttctc gacggcggct atgggcgcat
     1261 cggcagagct cccggcaatt tccagcgaac ctcccgccag cgcctgaata cctacgtcgg
     1321 acgcgcgaat gacgaccaaa tcgacatcga gtccctggcg ttcgaagaaa cccatcttcg
     1381 acgcaatcca cagtggacca tagccaagcg tcttggtagc cgagctgaca cgaagcttga
     1441 ttctttcttg tgacccgcac ggtggcgcat gcaggagaag cagcacgatc gttacaatga
     1501 cagaacactt cgtcaacatg gcgcctcctt ttggagagct gttcgcggcg gccgttaggt
     1561 aagaacatcg tcgccgcgcc atcgttcgat ggtcgcttcg tcgtagccga gtacttcgcc
     1621 gagaatctgg tcattgtcgg aaccgaatct attgggcaaa cggtagggct cttcctccag
     1681 ccctccaaac aaaatgccgc tgcgcgggat ctcaaacggg ccgatgaggg aatcgggaaa
     1741 cgccgcgaag tagtttcgat gccgcagttg cgggtccttc aataagtctg cgccggtttg
     1801 caccacgccc gcggcaacgc ccgcggattg gagcttggtc atcacatctt ccgcggttct
     1861 tgtctgcgtc caccgctgca cgatctgatc gagttcggat gaatgcttga gccgcgcctg
     1921 acaatcggca aaacgaggat cgccagccaa ttcggcttta cctatcacgc gacacagtgc
     1981 gcgccattcg tcatcgtcgc ggacggaaat cacgcaccag cgatcatcgc cctgacaccg
     2041 gtagcagcca tgcggcgccg ctccgccata ctgattgcct tggggttttg gatcatcgcc
     2101 gttaaccgaa gcttcgagat aggttgtgcc gagcgtgtaa gcggtgcctt cgatctgcgc
     2161 cagatcgatg gtaactcctc tccccgtccg gcggctattt aataaggccg cgatcatcaa
     2221 ggtcggcccg atgatgaatc ccagatgatc aggatatact ccctgactgc caatggggcg
     2281 gttctgttcg gggtgattcc acaaatacgt catgccggag aaggcagtta aattgaaccc
     2341 ccaggttcca taactgctct taggtccatc tgagccgaag ccgggcattt taagaatcgc
     2401 aagtttagga ttagctttgc gaagctcttc atcgcctaaa ccaagtttat ccaacactct
     2461 aggacggaaa ttttgcaaaa tcgcatcggc ttttccggcg agctctttga ttagccgctg
     2521 gccgatcgaa tgcttgagat tgacgctcag cgaccgaacg ccaagattgc actcgatgaa
     2581 gcgcggcgct gcgtcgatgt cttggtgttg gccgtaatgg cgaaaagtgt cgagtccgcc
     2641 ttttcgggac tcgattttga tgacctcagc gccgtagctc gccagcaacc tgccggcata
     2701 aggaccggca tacccggtcg tgaaggtgag gattcgcacc ccttccagta tgcgattcgc
     2761 gctcaacacg gttagtccga tctgttagcg atcatgtaaa ttcgcccgag attcgcagta
     2821 aagatcatct gtgtacggtt caagcgagtc atctgaattg atacttgagg ttttttagat
     2881 cacgccatcc gccgccagaa ctgccaattc attcagttcc aacccgagct cgccgcaaaa
     2941 gatctcttga ttgtgctggc ccaaaagcgg cgcgggcgcc ggcgccgcgc gttgaccatt
     3001 caccataaac ggacttcccg gttgctcgaa cgatccgatc acaggatgtg tcacggtggc
     3061 gaagaaacct cgcgcccgaa tgtgcgggtc cttcataaaa tccgatggcg tgttgaccgg
     3121 cagccccggt atgccgtgct tctgcatcag ttcggccaga tcttctttga gaaaacgaga
     3181 ggtaaacgaa gtcacctccg cgttgatata atcagcgtta gcacgccgca ggttgctgtt
     3241 gatccatttg ggatcgtcga gctccggcgc gtgatctccc cagactttga gcaacaacgg
     3301 ccaatgagcc tgcgtcacga acagcgcgat gtagccgttc ttgcattcaa acagattcgc
     3361 tggcgcattg tgctggtgct ggctgccggc gcgcttcatc acccgcccct cggcagaata
     3421 gttgaaggcc acatgttcgt gcagtgccaa actggcttga acggaagcgt caatgtacgc
     3481 accgatacct cgggtctccc gctgccagag agccaaggta atgccgtagg cggcgaatag
     3541 gctggcatag taatacgatt gagtttccgg cggattacag ggtgccatct tcgggtcgcc
     3601 gctgatgtag agcaatccac ccatagcggt gccgacgatg tccggcgcct tgaattgcgc
     3661 atagggtccg tcctggccga agcccgtaag cgacgccaga atgagtttgt tttgcatctc
     3721 gatcaaatcg ctataaccca aattgtgcga cgcgagaaaa cccggcgcga aagtttcgat
     3781 tacgacatcc gcactcttcg tgagacgtaa caataaccgc ttaccctgcg attttgttat
     3841 atctaatgta atacttcgct tgccggcatt gagataagcg aaacgaagac tgccttcgcg
     3901 gtgcgcatgg cccttggcaa agggcggctc ggagcgggcc gagtcgcctt gcggcggttc
     3961 gattttgatg acgtccatgc ccatgtcgcc gagcaatttg ccgcatagcg cgcccatgga
     4021 tgatgtcaga tcgaggacgc ggtagccggg aaggagcgcc gccgattcag tcgtattgat
     4081 catgctagtc tctgaaaact ctttccctac aggctgctca aaagatctca aatgcgaggc
     4141 gcgcgaaggt cggtgagcgg aggagtacgc ttcagtacgt tgaagcgagt cggacgagcg
     4201 caacgaagca tatgagtctt ttcagcagcc tgtcaggatt tgtaattctt aatgatgtgc
     4261 tgggcgagac gccaacgcaa aacttccgga gttccttcgg taatgcgaat actgcgcacc
     4321 tggcgatacc accactcgag cggcagatcc cgcatcagcc ctaatccgcc atgcacttga
     4381 atcgatcggt cgacgacccg actggccatt tcggcgcaca tgattttggt catgtaggat
     4441 aaatgccgga catcctcgcc gcggtcatga cgccaggcgc actcatagac catcagcctg
     4501 caggcgtgca gctccatcgc cgaatccgcg atcataaact gaatcgcctg ccggtcagcc
     4561 agcgggcggc caaaggtggt gcgcaccttg gaatattcga tcatcatatc cagcgcgcgg
     4621 ctggcgatgc cgactggacg cgcgccgtgg ccgcgaatgc gtccctcttg tatccagccc
     4681 tgggcgatcg caaagccctg gccctcgccg ccgatgatgt tcgccgccgg cacgcgcaca
     4741 tcttcgaaaa gaatttgtcc gggtgtgtcg cccatcatcg ttggccatag gcgctccaag
     4801 gtgacgcctg gcgctttcat atcgacgatg aaacaagtca cgccgccgcg cgcgcctttt
     4861 tccggatcgg tgacagcgat cacctgagcg taatccgccg acttggcgcc ggtgatgaaa
     4921 cgttttgtgc cattgataat gtagtgatcg ccatttttta cggcgcgggt tttcatccct
     4981 gccggatcgc tgccggcatc cggttcggtc tgcgcgaagc aaacccgttt ctcgccgcga
     5041 atcaccggat aaagaaaccg ttccttttgt tcctcgttac aatgaaacag caccggccgg
     5101 acttcgggcc cgaagatcgg cgcataacga aacggaatcg caacagtgcg cgccacctct
     5161 tccatgatga cgcaacggct catcagatcg agaccggcgc cgccatactc aggcggcacg
     5221 tccaacaacc aaagaccaat cgcctttgcc ttctcctgga gcggtttgat gtattgttcc
     5281 ggcatatcct cgccctcggg ccggcactcg cgctccaacg gaatcagctc cttgtcgacg
     5341 aatttacgaa ccgtttcttt taatagctgc aactcttcgg gtaaattgaa gtccacctta
     5401 gctccgtctt aactttcttc cgcaattgga ccgggctcac caaacccggt ccaattgcga
     5461 ttacttcaga ttcaatttaa aactcaaacc tacttggcct cgcgattgta cgcgcccagt
     5521 ccgggccgat aggtcttctc atcgaggaac tgtttcatcc ccgtctcacg ccccttttcc
     5581 gggtcggtca aacgaagcgc agtgcctttg gcgctcatat attcttctgc ctggttgtag
     5641 tccatggtgc ggcaatactt gaagacttcc ttggcggctc taagcacttg aggatttttt
     5701 tccatcagtt tcttggcaag cttgacagtc tcgtcgcgca gttgttcctt cggcacggcg
     5761 taattgatca gcttcaattc cgcggccttt ttgccgtcaa acggatcgcc ggtcatgatg
     5821 taccacatcg catctcggta gcacatggca tcggcgagca cgcggctcac caaaccgccg
     5881 gggaaaatcc cccagttgat ttcgctcaga ccaaagaccg catcctcggc ggcaatcgcg
     5941 aaatcgcacg caatcaaggg cgtaaacgcg ccgccaaagc aaaagccgtt gaccatcgcg
     6001 atggttggtt tcggaaaagt aaatagccgg cgccagcgcc actcttgaga agcccagctg
     6061 gctttgcgtc tttccaccgg attggtatcg gttcccctga aatattcgcg caggtcctga
     6121 ccggcgcacc agctcggacc ggcgccggtg ataacgacga ctttgctgtc tttatccact
     6181 tccagctcgg taagcgcctc gagcatgtcg taatgcaatt gcgggttcat cgcattacgc
     6241 ttttcgggac gattgagaat gacccaggca atgccttggt ctttttcgac tttgacggtt
     6301 ttaaaatctt ttacttctgc catcgctctt tcctccttat gttctcacgt tagtttgatt
     6361 tgtgttgttc agatgacgct gttcccgtcc atatcaagtc gcctatcgtt ggtaaagtgt
     6421 ttttacgaaa ccgctcgcct cgatctcgcg cagcaggcta tcatcgacaa acattttagg
     6481 atccgcgcct ttggctttcg gattgtcctt ttcgatgaag ccgagcaccg tttcgacgcc
     6541 gcgcaatgac ggatatggca cagactcgta gtatttcgcg attaacagat ctatggagcg
     6601 ttccaggttt ttttcctgct cgggaccgat gcgaagcttc ttcttgacga tgtccaaact
     6661 ctcctgcttg ttatgcttga cgaacgcaat tccttcgacg taacccttga gaaatcgcac
     6721 cactttgtcg cgattgttct tgatgtactg gcgcgtcgtc gcgaccatcg tatgcaggta
     6781 gtaaatatcc gtatccgcca tgtcgagcaa cactctataa ccgcgatctt cggcgacgaa
     6841 catcgaagga atcgtcacca cggctccttc gatacctccc ttatcgaggc tcgccagcat
     6901 actcggcgag gagccgagtt gaagaacctg gacgtcattc acgctcatct tccagcgctg
     6961 cagcatcatg agcagaaccg aatgggaaac cgcaccgatg cgagtgacgc caacccgctt
     7021 tcccttcagc tccgcgggcg tctttatgtc cggtttggta ataagccgtt gcagtccttt
     7081 gttgacaaca ctggcgacca tgactgtatc gccgccacgc aacgaagctg cgaccaacac
     7141 atcgccggct gaattcacca tctgcacgtc gttggcgatg agcgcactga tgccggcggt
     7201 cccaccctgt ccggtgacga tcacttcagg atcgatgccg tgtttgcgaa agagtccttt
     7261 tgtttcagcc acccacacag cggtgtgcag accgctcacc gagctcaacc ccattctaac
     7321 tttgtcctgc gccatcgcac gccaaccgac gcaaagaaaa aaaacagtta tggcccatac
     7381 gaatgttttt cgattcatcg ctacgttctc cgtttagctc ttgcccactt tcgccggtgc
     7441 ctggtgccga taagcaggat cgatatactt cgggcttata gtgtgtccac cgtcgcgttg
     7501 gctatgcata tagatgaaat cggcggtctc taaaaa
//
LOCUS       DASUVS010000097         9021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1562272, whole genome shotgun sequence.
ACCESSION   DASUVS010000097 DASUVS010000000
VERSION     DASUVS010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9021
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562272"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VF182_05360"
     CDS             complement(<1..459)
                     /locus_tag="VF182_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two pore domain potassium channel family
                     protein"
                     /protein_id="HEX6436537.1"
                     /translation="MNYQHISFLRRRLLVRHHPSAFLLAAQLLSLMLYAAFDGIHSGR
                     ALLGAFSVIVLALAVWVVSRSPAINWIAWVLAIPAFILSVLSALFVDPLLLVWSSLLE
                     AALYFYAAGSLIGYMMQDRRVTTDELFAAGATFTLIAWGFAYVYLVCQTWY"
     gene            1040..2290
                     /locus_tag="VF182_05365"
     CDS             1040..2290
                     /locus_tag="VF182_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436538.1"
                     /translation="MSRNRSLPQLIIVCALLSVGAAACGSGTPQARWSHQDWPTGHYR
                     VVEGWPKPLPDTRHSHEGWTWGSFGGVYAESPDRIWVAMRGELPLPKGAAPWTPYAAL
                     NPSRGNATGNSDGLNATCQPDKKRGWERRFEHSIIVFDRQGNLVDEWPHLEALFSKNP
                     CGRGPHQIKISPYDKAQHVWIIDDQLHMIYRFTYDGKLVHSKGQLGVRGRGPNTFDRP
                     TDIAWLPDGTYFITDGYGGTRVVKYDANDKFIMDWGGPPKDPKNPGPNEWNTVHSIAI
                     SADRRLFVIDRGHARMQVFDENGKFLDMWPLKSPHWPANQPTLMVNHFIDEKGFIWVG
                     DAPTSRILKFDLNGNFLYAWGAPGGQPGRLNCSHGITTDQLGNLYLADCFAGRLQKFE
                     PISGADPDKIAGQILRTWDTWKRP"
     gene            complement(2301..2747)
                     /locus_tag="VF182_05370"
     CDS             complement(2301..2747)
                     /locus_tag="VF182_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6436539.1"
                     /translation="MSGNTIRLHRVLRATPERLYKAFVDGDAMSKWLPPNGFTGKVHH
                     IDAKVGGTYKMSFTNFSTGKSHSFGGQFLELMPHERIRYTDRFDDPGLPGEMQTTVTL
                     KKVSCGTELAIVQEGVPSVIPAEACYLGWQESLILLAKLVEPEIPD"
     gene            complement(2911..3597)
                     /locus_tag="VF182_05375"
     CDS             complement(2911..3597)
                     /locus_tag="VF182_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX6436540.1"
                     /translation="MLEANLIQIAGVIDAAEAAMLQQCEIRYLGFPLRLPIHRQDLTE
                     DEAAAIIKSLAPPVFGVLITYLDEASEIAAFCHALGARIVQLHGDIHRDELKHLKTVD
                     PDLTIIKSLVVGMHDDKALEAMVGELSPLVDAFITDTFDPKTGASGATGKTHDWRVSR
                     RLVKLADRPVILAGGLTPENVKRAILEVRPAGVDSHTGVEDSTGRKSRGKVNKFLSEA
                     NEAFELVKAK"
     gene            complement(3681..5801)
                     /locus_tag="VF182_05380"
     CDS             complement(3681..5801)
                     /locus_tag="VF182_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX6436541.1"
                     /translation="MANNSPLTPLFEADRLAIVGASDRNHYAVNIIKNLQNMGFESSR
                     IVPINPGRPEVFGLKAYPSVLEVPGDIPLAVIAVNTNTVVGVVEELGKKRVKAGVIFA
                     DGFAEGGEQGKKLQQQLTDAARAGGLKLLGPNCMGFVSVPAKLGIWGGELPKNLRAGN
                     IGCIFQSSGMMNLIMILGTKRGFGFHLCASGGNEIILNSADYLEYQADCPEIDVIAAY
                     FEAAPKEPQRFAAALDRAIANGKSVLILRAGRTDRAKRNVIAHTGQLAGTAATWDAFF
                     EQHGAILIDDLDDLIDTIALFAGAKVRPDKHERGVGLVTISGGDCTLLCDIADQEGVA
                     LAELSPETQNILTASLDKPTLLGNPLDVEDLQRMKPAAFDLCLEKFFQEPNIDMLGIR
                     LNLPETITPASEALYGKIADLSKANDKRVFVLTRATEPPAEVWHKRLNDLGLTFTGDY
                     RKSLRALGRLRRNEVDRANGRFIAATRAGAAPKRAELGHGVLSYGATETLLRAYGITA
                     TQTVLAHSADEAVTASEQVGYPCVLKVASVDLPHKTEFGALRLGLDDRESVRRAYDEM
                     LSKVRALKPGANIEGVLVQKQIKGVECLLGISRDEQLGPTLVIGLGGVFVEILADVAL
                     RIPPISATEARGALENLQGAKVFAGVRGVPPADIDALAEMAARLSWLAYDLRDEIAEL
                     DLNPVIILPKGQGAFAVDALVVMR"
     gene            6165..7016
                     /locus_tag="VF182_05385"
     CDS             6165..7016
                     /locus_tag="VF182_05385"
                     /inference="COORDINATES: protein motif:HMM:NF006487.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6436542.1"
                     /translation="MLPALLRIIARLPLRWLHRIGVMVGWVMYRTWPTHARRLRENLR
                     ASAICASETEYHALLHETVRESGKAAVEVAKVWFGPDREINKLIMGCQGWADVEAARK
                     LGKGIIFLSPHLGCFEIAGQYISQRMPLMVLYRPPRQKWVKSLIIAGRSREQLRLAPT
                     NRRGVQLLLKTLHSGEAIALLPDQAPKSAGVWADFFGRPAYTMSLVRKLQQSTGASLI
                     SVFSKRLPCGTGFELEFESVASENFDESTLNRLVENLVRRCPEQYLWSYNRHKVPRLA
                     SQQPAQS"
     gene            complement(7013..8389)
                     /locus_tag="VF182_05390"
     CDS             complement(7013..8389)
                     /locus_tag="VF182_05390"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6436543.1"
                     /translation="MATPPPVTDTLARFVAETDYSAISDSALSNAKMHLLDTIGAGLA
                     AASTPVAAIAFDYCKRIGHSQDASIWGTNEKASAPMAAFTNGLLAHALDFDDWDAYLR
                     AGHPSSVITAAALPLGENIGASGKDLLKAYVLGIEVLTQLAAGTPELHKRGFHSTPVW
                     GGIGAVIAAVSLLKLDSEKIKAALGVAASGAAGIQRQQGSMVKPFHAGNAARNGVEAA
                     LLAQQGFTADPNIIEAPRGFCDTFFGPGTCDYEKMLTGIGQPYFLESPGLGLKMHPCS
                     APQFLAADAALQLKREHGIDFAAVAKIEVSIPPLRYQRHFHAEVKTGLRGKFAINYVV
                     ALGVLEGKLDNDTFTDAKANDPLVQQALRRVQVIIDDSIPEPGTYCPVTIELKSGKRL
                     SHTARIAKGHPENPMSESEVLTKFRNNARDIISADRSEQLIAALQNLEEIGDLRELAG
                     LLRAREYP"
     gene            8503..>9021
                     /locus_tag="VF182_05395"
     CDS             8503..>9021
                     /locus_tag="VF182_05395"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX6436544.1"
                     /translation="MKGCLDGIRVLELGNFISGPYGAMLLADMGAEVIKVENPKGGDP
                     FRGWDFGGDQPNFWAYNRGKKSVTLNLQTAEGQEIFYRLVKQADVVMDNYRPGVTKKL
                     GIDFESLQKVNEKIVCCSITGTGPTGPYVKRPAYDTVGQGMGGMMSVLMDPQNPKPVG
                     PAYADCLSGMFSA"
BASE COUNT         1942 a         2565 c         2548 g         1966 t
ORIGIN      
        1 gtaccaggtc tggcagacca agtacacata ggcgaatccc cacgcgatta aagtgaacgt
       61 tgcgccagcg gcgaataact cgtcggtggt cacacgacga tcctgcatca tatacccaat
      121 cagactaccc gctgcataaa aatacagcgc cgcttccaag agcgatgacc aaaccaacaa
      181 gagcggatcg acaaacagcg ccgaaagcac tgacaatata aacgccggaa tcgcgaggac
      241 ccatgctatc cagttgatcg ccgggctgcg gctgacgacc cagactgcaa gcgccaggac
      301 gattaccgag aacgcgccga gcaaagcccg gccgctgtgg atcccgtcaa acgcggcgta
      361 cagcatcagg ctcaataatt gcgcggcaag caggaatgcg gacgggtggt ggcgcacaag
      421 aagacgccgc cgcagaaacg agatgtgttg ataattcatg gcgctgcgtt atgggtcatg
      481 gaattccacg atattgcacg ccacattgat ggaacaacaa cgaggatgca agccttatct
      541 ggtgcaataa ggttggtcaa aatgcgctgg atcagaattg attcacggat tgtgactgca
      601 taaggccaga aacatattgc acggattggc tgggtgacat gtgtgcagat tcatcgagaa
      661 agattcagaa atttagaatt taaggccggg tcttgcaatc acacagtttc ttctccttcc
      721 tcccatccgt tggagccggt ggctcaccgt cgcctcatgg gcccggggcg gatacgcagc
      781 gatcttgtga agccgagacc cataatcttg tacgccgaac gattcctcga tcgtcgcttc
      841 ctttcggggc ctctccactt cggccggatt gctttggatt gcgcagtggc aggactcacc
      901 cacaaacaaa cgatgattga caacttttcc ggtcctgcgc tattcctcag ccaaaaatag
      961 gttgctttaa gattgcgaat actgagtgtg ggttcttcat tgttggttga aagcaaacaa
     1021 cgattcctgt ggaggcctca tgtctcgcaa ccgttctttg ccgcaactga tcattgtgtg
     1081 cgcgttgtta tccgtcggtg cagcggcttg tggcagtgga acgccgcagg cgcgatggag
     1141 tcatcaagac tggcccaccg gtcactatag agtggttgaa ggatggccta agccgttgcc
     1201 ggacactcgt cactcgcacg agggatggac ctggggatct tttggcggcg tgtacgctga
     1261 aagcccggac cgaatctggg tcgccatgcg cggcgagctg ccgctgccga agggcgccgc
     1321 cccgtggaca ccctatgcgg cgctcaatcc gtcgcgcggg aacgccaccg gcaactcgga
     1381 cggattaaac gcgacatgcc agcctgacaa gaagcgcgga tgggagcgcc ggttcgagca
     1441 ctcgatcatc gtgttcgatc gccaaggcaa cctcgtcgac gaatggccgc atctcgaagc
     1501 gttgttttcc aaaaatccat gcggccgtgg gccgcatcag atcaagatca gcccctacga
     1561 caaagcccag cacgtctgga tcatcgacga ccagctgcac atgatttacc gctttaccta
     1621 cgacggtaag ttggtgcaca gcaaagggca gctcggtgtg cgcggccgcg gacccaacac
     1681 cttcgaccgg ccgacggaca tcgcctggct gcccgacggg acctacttca tcaccgacgg
     1741 ctacggcggc acccgcgtcg tcaagtacga cgccaacgac aagttcatca tggattgggg
     1801 cggcccgccc aaggatccga agaaccccgg gccgaacgag tggaatacgg tgcacagcat
     1861 cgcgatcagc gccgaccggc ggctcttcgt gatcgaccgc ggccacgcgc ggatgcaggt
     1921 cttcgacgag aacggcaagt tcctcgatat gtggccgctc aagtcaccgc actggcccgc
     1981 caatcagccg acgctgatgg tgaatcactt catcgacgaa aaagggttca tctgggtcgg
     2041 cgatgcgccc acgagccgga tccttaagtt cgatctgaac gggaattttc tctacgcctg
     2101 gggcgctccc ggcggccaac ccggccggct gaactgttca cacgggatca ccacggatca
     2161 actcggtaat ctctatctcg cagactgctt cgcaggccgg ctgcagaagt tcgaaccgat
     2221 ctcgggggcg gatccagaca agatcgcagg ccagattctc cggacgtggg atacctggaa
     2281 gcgcccttga gcgcatcctc ttaatccggt atttcgggct cgaccagttt tgctagaagg
     2341 atgagcgact cctgccagcc aaggtagcaa gcctcggcgg gaatgacgct cggcactcct
     2401 tcctgcacga tagccaactc ggttccacag gacactttct tcagagtgac ggtcgtctgc
     2461 atctctccgg gcagacccgg gtcgtcgaat ctgtccgtgt aacggatgcg ctcgtgtggc
     2521 atcagttcaa gaaactgtcc gccgaaggaa tgactcttgc cggtgctgaa gttcgtaaaa
     2581 gacatcttgt aggtgccgcc gactttggca tcgatgtggt gaacctttcc ggtaaacccg
     2641 ttcggcggaa gccatttgga catggcgtcg ccatccacaa acgccttgta caatctttcg
     2701 ggggtcgcac ggagcacgcg gtgaagccgg atagtatttc ctgacatgat ttttctcctt
     2761 taggtgggtt ctctgttgcc gctatgccgc tccgatattt tccaatcctc gttgcggttt
     2821 tcgctttcat ctctatgacg aatgaactca aacagccagg acagtatggc taaatttttt
     2881 ttcgcattac ctcaccggcg gcgacgatct ctattttgcc ttcacgagct caaaagcttc
     2941 atttgcttcc gacaagaatt tattgacttt tccccggctc ttgcggccgg tcgagtcttc
     3001 gacgccggta tgagagtcga ctccggccgg ccgtacttcg agaatggctc tcttgacgtt
     3061 ctccggtgtc agtccgccgg cgagaataac cggtcgatcg gcaagtttta ccaggcgccg
     3121 acttacgcgc cagtcatgag tcttgccggt cgcgcccgat gcgccggttt ttggatcgaa
     3181 cgtatcggtg atgaaggcat cgaccaacgg tgataactca ccgaccattg cttcgagagc
     3241 cttgtcatca tgcattccga cgaccagact tttgatgatg gttaaatccg gatcgacggt
     3301 ttttagatgc ttcagctcat cgcggtggat atcgccatgg agctgtacga tgcgggcccc
     3361 gagggcgtga cagaaagcgg cgatctcact cgcctcatcg aggtaggtga tcagcactcc
     3421 gaataccggc ggggcgaggc tcttgatgat cgccgccgcc tcatcctccg tgagatcctg
     3481 gcgatgaatt gggagccgca gcggaaatcc caagtagcga atctcgcatt gctgaagcat
     3541 tgccgcctcg gccgcgtcga tcaccccggc aatctggatt aagttggctt cgagcatgta
     3601 aacccgtcac gacaattcgc ggttaaccgt taccagagta atcaaaacgc ttcgcaattt
     3661 ttcgccaagg cggcaagggc tcagcgcatg accaccaacg cgtctaccgc gaacgcgccc
     3721 tgacctttcg gcagtatgat tactggattt aaatccagct cggcgatctc gtcgcgcaga
     3781 tcgtaggcga gccaggaaag ccgcgcggcc atttcggcca gcgcgtcgat gtcggcgggc
     3841 ggcaccccac gcactccggc gaaaactttt gcgccttgca agttttccag cgctccgcgc
     3901 gcttcggtgg cgctgatcgg cggaatgcgc agcgccacgt cggcgagaat ttcgacgaac
     3961 acgccgccga ggccgatgac caacgtgggc cccagttgct cgtcgcggga aatccccagc
     4021 agacactcga cgcctttgat ttgcttctgc accagaacgc cttcgatgtt ggccccaggc
     4081 ttgagtgcgc gcacctttga tagcatttca tcgtaggcgc gccgaacact ctcacgatcg
     4141 tctaaaccca agcgcagcgc gccgaattcg gttttgtgcg gcagatccac cgatgcgact
     4201 ttgagaacgc aggggtagcc gacttgctcc gaagccgtaa ccgcttcatc cgccgaatgc
     4261 gccaataccg tctgagtcgc cgttataccg taggctcgca acagtgtttc ggttgcgcca
     4321 tacgacagca cgccgtggcc cagctcagcg cgcttcggcg ccgcgccggc gcgggtcgct
     4381 gcgatgaagc ggccgttggc gcgatccact tcgttgcgcc gtaatcgccc aagcgcgcgc
     4441 agtgatttgc gatagtcgcc ggtgaaggtc aagcctaaat cgttgagccg tttgtgccag
     4501 acttccgcgg gcggttcggt ggcgcgtgtg agcacgaaga cacgcttgtc gttggcttta
     4561 ctgagatcgg caattttgcc gtagagcgcc tcagatgccg gcgtaattgt ttcgggaaga
     4621 ttcaggcgga tccccagcat atcgatattg ggctcttgga aaaatttctc caggcacaga
     4681 tcaaaagccg ccggcttcat tcgttgcagg tcttcgacgt ccaggggatt tcccagcaag
     4741 gtcggcttgt cgagacttgc cgtcaaaata ttctgggttt cggggctgag ctcggcgagc
     4801 gcaacaccct cctgatcggc aatgtcgcac agcaaggtgc aatcgccgcc ggagatggtt
     4861 accagaccga cgccgcgctc gtgtttatcc ggccggacct tggcgcccgc aaacagcgcg
     4921 atggtgtcga tcagatcatc gagatcgtcg atcagaatag cgccgtgctg ctcaaaaaat
     4981 gcgtcccagg tggcagccgt gcccgccagt tggccggtgt gcgcgatcac attgcgtttg
     5041 gctcggtcgg ttcggccggc gcgcagtatc agcacgcttt tgccgttggc gatggcgcgg
     5101 tcgagcgcag cagcaaatct ttgtggttct ttcggcgctg cctcgaaata ggcggcgatc
     5161 acgtcgatct ccggacagtc cgcttgatac tccagatagt cggcggaatt tagaataatt
     5221 tcattgccgc cgctggcgca gaggtgaaat ccaaaaccgc gcttggtgcc caggatcatg
     5281 atcaagttca tcatgccgct gctttggaaa atgcagccga tatttccggc gcggagattc
     5341 ttcggcagtt cgccgcccca aatacccagc ttggccggca ccgaaacaaa acccatgcaa
     5401 ttgggaccaa gtagtttcaa accgccggct ctggcggcat cggttaattg ttgttgtaat
     5461 tttttccctt gctcaccgcc ttcggcgaag ccgtcggcaa aaattactcc ggctttgacc
     5521 ctttttttgc ctaactcttc gaccacgccg accaccgtgt tggtattgac cgcgatcact
     5581 gcgagaggaa tatcccccgg cacttcgaga acgctgggat atgctttcaa gccgaagact
     5641 tccggccgtc ccggattgat cggcacgatc cgagaagact cgaaacccat gttctgcaag
     5701 ttcttgatga tattgactgc gtaatggttg cgatcgctgg cgccgacgat ggcgagacga
     5761 tccgcctcaa agagcggagt caaaggactg ttgttggcca aagtattctc ctataacaga
     5821 ctgctgaaaa agcccatatg ccaagatgaa ttatgaagct gaaaactgaa ttccgctttc
     5881 agatttccgc tttcatcctt gtgattcgtg cgcctgccct ctgcctcctt tgagcagcct
     5941 gcaccagagt ctttcagaat tgcgactaat cgagatggaa ttcccatacc gcagccggaa
     6001 acggatgtcc aagcaacgca gaggcaaaat gcaaagttcc ccggtccaaa gaagcgcttt
     6061 tatctctgcg gatctgtttt agagttgccc gaacgccttc taggagctat cttaactaga
     6121 ctgtctacta ccatcgaaat taccgcttat catccttgtt catcgtgttg cctgcgttac
     6181 taagaataat cgctcgactg ccgcttcgtt ggttgcatcg cattggcgtg atggtcggct
     6241 gggtcatgta ccgaacttgg ccgacccatg cgcgacgatt gagagagaat ctgcgcgcca
     6301 gcgcgatttg cgccagcgag acggaatatc acgcgctgct tcatgaaacc gttcgtgagt
     6361 cgggcaaagc tgcggtggag gtggcaaaag tttggttcgg ccccgatcgt gaaatcaaca
     6421 agctgatcat gggatgccaa ggttgggccg acgtggaagc agcgcgaaag ctcggcaaag
     6481 gaataatatt cctttcgcct catctgggtt gcttcgaaat tgccggtcag tacatttccc
     6541 agcgcatgcc gctgatggtt ctctaccggc cgccacggca aaaatgggtc aagtcgctga
     6601 tcatcgccgg ccgcagccgc gaacaactgc gcttggcgcc gaccaatcga agaggcgttc
     6661 aattgctttt gaagactctg catagcggtg aagccattgc gttgctgccg gatcaagcgc
     6721 ccaagtccgc cggagtgtgg gccgatttct ttggccgtcc cgcctacact atgtctttag
     6781 tgcgaaaatt gcagcaaagt accggcgcct cgctcatcag tgtctttagc aagcggctgc
     6841 cttgcggcac cggcttcgag ctcgagttcg agagtgtcgc aagcgagaat ttcgacgaga
     6901 gcacgctcaa tcgcttggtt gaaaacctgg tgcggcgctg tcccgagcaa tacttgtgga
     6961 gttacaatcg ccacaaggtg ccgcgcttgg ccagccaaca acccgcgcag agttaaggat
     7021 attcacgcgc ccgcaacaaa cccgccagtt cgcgcaggtc gccaatttct tctagattct
     7081 gcaacgccgc aatcaactgt tcagaacgat ccgctgagat gatgtctcta gcgttgttcc
     7141 tgaacttggt caggacttcg ctttcgctca tgggattttc cggatgacct ttggcgattc
     7201 gcgcggtgtg gctcagacgc ttgccgctct tcagttcgat cgttaccggg cagtaggtgc
     7261 cgggctcggg aattgaatca tcgatgatca cctgaactct gcggagcgct tgctgaacca
     7321 gcggatcgtt ggcttttgca tcggtgaaag tatcattgtc caatttgcct tcaaggacgc
     7381 caagcgcaac gacataattg atcgcgaatt ttccgcgcag gccggttttc acttccgcgt
     7441 ggaaatgccg ttggtagcgc agcggcggaa tactcacctc gatcttggcg acagcggcaa
     7501 agtcaatccc atgctcgcgc tttagttgca gggccgcatc cgccgcaaga aattgcggcg
     7561 cgctgcacgg gtgcatcttc agcccgagcc cgggggattc gagaaaatac ggctgaccga
     7621 tgcctgtcaa catcttttcg taatcgcagg ttcccgggcc gaagaatgtg tcgcaaaagc
     7681 cgcgcggcgc ctcgatgata ttgggatcgg cggtaaaacc ttgttgcgca agaagcgcgg
     7741 cttcgacacc gttgcgcgcg gcattgccgg catgaaacgg cttgaccatg gaaccttgct
     7801 ggcgctggat tcctgcggct ccggaggcag ccacgccaag agcggctttg attttttccg
     7861 aatccaattt gagcaaactg accgctgcga tgaccgcgcc gatcccgccc cacaccggcg
     7921 tgctgtgaaa gccgcgtttg tgcagctcgg gagtgcccgc ggccaactgc gtgagcactt
     7981 caatcccaag aacataggct ttcaaaagat cttttcccga ggcgccgata ttctcgccca
     8041 gcgggagcgc ggccgcggtg atgacgctgc tcggatgacc cgcacgcaag taagcatccc
     8101 agtcatcgaa atccaaagcg tgagcgagta agccgttggt aaaggcggcc attggcgccg
     8161 aagctttctc gttcgttccc cagatggacg cgtcctgcga atgtccgatg cgcttgcagt
     8221 aatcgaaggc gatcgccgca accggcgtgc tcgccgccgc caagccagcg ccgatggtat
     8281 cgagcaggtg cattttggcg ttgctcaaag cactatcgga gatggccgaa tagtcggtct
     8341 cggcgacgaa acgagctaag gtgtcggtga cgggtggcgg cgttgccatc ttgttctcct
     8401 gcgaattgga aggtattaaa gtccaatatc gaaatcgccc acgactgtct atcggacgtc
     8461 gctgccggcg acagggtgag taaaaattaa agaggtaaag gcatgaaagg ttgtttggac
     8521 ggcatcagag ttttggagct tggcaatttt atttctggcc cttatggcgc gatgttgctc
     8581 gcggatatgg gcgccgaagt gatcaaggtt gagaatccca aaggcggcga tccatttcgc
     8641 ggctgggatt ttggcggcga ccaacccaat ttctgggctt acaaccgcgg caaaaaaagt
     8701 gtgaccttga atctgcaaac agccgaaggc caggaaattt tttatcgctt ggtgaagcag
     8761 gccgacgtag tcatggataa ttaccgtcca ggcgtaacca agaagctggg catcgatttc
     8821 gaatcgctgc agaaggtcaa tgaaaaaatt gtatgctgtt cgatcacagg taccggcccg
     8881 accggtccgt acgtcaaacg gcccgcctat gacacggtag gtcaaggcat gggcggcatg
     8941 atgagtgttc tcatggatcc acaaaaccct aagccggtcg gcccggcgta tgccgactgc
     9001 ttgagcggca tgttctcggc g
//
LOCUS       DASUVS010000098        11295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1562514, whole genome shotgun sequence.
ACCESSION   DASUVS010000098 DASUVS010000000
VERSION     DASUVS010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11295
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562514"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VF182_05400"
     CDS             complement(<1..576)
                     /locus_tag="VF182_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX6436545.1"
                     /translation="MDAYLKTLNRIYNLRGGIIDLRLERMERALALFDHPEKTFPSFH
                     IAGTNGKGSTAAILHRILCSAGYRTALYTSPHLVSFTERIRIDDEEITADEVVALAEE
                     IWRRTETAHILLTFFEIVTVMAFLYFARRRVDAAVIEVGLGGRFDATNLVRSIVTVIT
                     TISRDHEAFLGFDIASIAREKAGIIKKGVPVV"
     gene            complement(615..1436)
                     /gene="trpA"
                     /locus_tag="VF182_05405"
     CDS             complement(615..1436)
                     /gene="trpA"
                     /locus_tag="VF182_05405"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX6436546.1"
                     /translation="MSRIEKKFSELKKADEAALIPFITAGDPDLQTTLQIMRALETAG
                     ADCIELGIPFSDPTADGPTIQRASERALKNKIALPEILQMLRRFRSTSEIPVILFGYF
                     NPFFRFGLKEFSRQSAQAGADGVLCVDLPPEESEELKRWTDAEGMDLIFLLSPASGKE
                     RLELVARKGRGFIYYVSVTGVTGARQSLDQQLRSQVAQVRRMTAIPVGVGFGISTPQQ
                     AGWIVQFADAVVIGSALVESIERANGSLAKAQAAGDFVSKIKRAMKRSRRNGRKH"
     gene            complement(1513..2736)
                     /gene="trpB"
                     /locus_tag="VF182_05410"
     CDS             complement(1513..2736)
                     /gene="trpB"
                     /locus_tag="VF182_05410"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX6436547.1"
                     /translation="MKKSNASSAPLKKLPDQHGHFGPYGGRYVAETLMPALMELERAY
                     AKARRDSAFRKMFHRHLREYAGRETPLYFAERLTEQLGGAKIYLKREDLCHTGAHKIN
                     NTIGQILLARRMKKARIIAETGAGQHGVATATVAARFGMQCEIFMGKEDVARQSLNVF
                     RMKLLGAQVRVVESGSKTLKDAMNEALRDWVTNVFDTYYLIGSVAGPHPYPMMVRDFQ
                     SVIGQEARRQILRQEGRLPDCLVACVGGGSNSMGLFYPFVQDPTVRMIGVEAAGRGLR
                     SGKHAASVLGGEVGVLHGSKSYILQDNGGQIRETHSIAAGLDYPGVGPELSYLKDLGR
                     VEFASATDRAAMEAIQFLAQVEGIIPALESAHAIAAVMQLAPRLGKNKTIVVNLSGRG
                     DKDMGTVGDYLGVKF"
     gene            complement(2690..3319)
                     /locus_tag="VF182_05415"
     CDS             complement(2690..3319)
                     /locus_tag="VF182_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX6436548.1"
                     /translation="MVKVKVCGITNLADAEKALEYGADALGFNFYPPSSRSLAPEKAG
                     AILRQLPADAWNVALFVNEAKERIKQILAKGTLPEGRCGYQTIQFHGDESADDCRGWG
                     IQVIKAFRLKDKQSLKGIHDFPADYYLLDSWSSGYGGSGMSFPWEWLEGFDTGKIILS
                     GGLDLDNVAEAVRRLHPYGVDVCSGVEVRPGVKDHEKIKRFISAAKETA"
     gene            complement(3387..4166)
                     /gene="trpC"
                     /locus_tag="VF182_05420"
     CDS             complement(3387..4166)
                     /gene="trpC"
                     /locus_tag="VF182_05420"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX6436549.1"
                     /translation="MADFLANIVEHVRTVLEQRQKQTPLAALHDRPLYYRATRGFARE
                     LSGGSRRIVAEIKRSSPSKGLIRADFNPVVIARDYADHGASAISVLTEEKFFQGSLTY
                     LEEIKGAVAVPLLRKDFIIDPYQLTEARSYGADAILLIAALLESTVLRDLRETATALS
                     LDSVVEVHNEEELERALDAGAEIIGINNRNLKTFEVSLATTERLAPRVPGGTLIISES
                     GIDGAEQIRQVERWGIHSFLIGESLMRAPNPGEKLQELLRS"
     gene            complement(4171..5187)
                     /gene="trpD"
                     /locus_tag="VF182_05425"
     CDS             complement(4171..5187)
                     /gene="trpD"
                     /locus_tag="VF182_05425"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX6436550.1"
                     /translation="MNIREAIERLVRRNNLSEEETIDVINQIMTGEATPLQVASFLTA
                     LRMKGETVEEITGAARVMREKAHRVQVGSKVVLDTCGTGGDQKGTFNISTASAFVAAG
                     AGVEVAKHGNRSVSSQSGSADVLAALGVRVDAPRERVESCIQQIGIGFLFAPLLHEAM
                     KYAVQPRRDIGIRTVFNLLGPLTNPAMANHQLIGVYDGELVSVIAHVLRNLGSARAMV
                     VHGMEGLDEISLCGPTKIAELRHGEVKEYTVEPEKIGFKIYPLEALHAGSATESAVII
                     KRILQGDKGPARDVVLLNSGAALYVSGKAETLEHGIRVAADSIDSGRARQKLEQLAQL
                     TNAA"
     gene            complement(5206..5892)
                     /locus_tag="VF182_05430"
     CDS             complement(5206..5892)
                     /locus_tag="VF182_05430"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX6436551.1"
                     /translation="MAENRKGKDKRFYFSRMQFILLGCGFGVASMIVFFLGMLVGQGM
                     EERKIVKPAEPVMKIPIRPAQGTSSAFSGPKEEITFYDTLTRPAPAQARNEEEPREAK
                     KAEKNSKAEARERLPARQEVASIAKRAEDKPAAAVSKSRPAEADAKESHRPWTVQVNA
                     FPDEKSAKTWVDRLRDKGYNAYLSESRIQGRVWYRVRVGRFESRDEAEKTQEALQRKE
                     HLTKAFATSR"
     gene            complement(5927..7615)
                     /gene="argS"
                     /locus_tag="VF182_05435"
     CDS             complement(5927..7615)
                     /gene="argS"
                     /locus_tag="VF182_05435"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX6436552.1"
                     /translation="MRKHLAGLLTRAIEKISKAGDLSSTEIPALVLELPKQREFGDLA
                     SNIAMHWAKAAKKPPRVIADAIVKNIEDPEGMLARIDIAGPGFLNFTFSQKFYYQRLR
                     DLVTGKDTQLDLGHGKKVQIEFASVNPTGPLHVGHGRVAVIGDVLARLHEATGFNVER
                     EYYVNDAGNQMENLGRSVYARYRELFGDSCELPEGGYPGEYVKDIAAEIKKQHGDKFL
                     SQSQEAAVAFFRRYGGDSLLATIRRQLSDFHIRFDSFFSEKAMRERGEIAESIELLRD
                     RGLVYSKDGAEWFQSTAFGDDKDRTVIKSDGELTYFAADIAYHRNKLERGFGKLINVW
                     GADHHGYVPRLKASLRGLGYDDSILRVVLVQMVQLTRAGEPVRMGKRSGEFVALQEVL
                     DEVGRDAARFFFLMRKTDSHLDFDLDLAKQQSSDNPVFYVQYAHARVASIFEQAHRNG
                     IFLDAADIGTIALERLELAEEIELIRRMAQFNDVLEECVRELEPHRMVFFLLDLAGEF
                     HRYYNRTRILSEDRELTAARMLLAQMVQLTVRRGLTLLGIDAPMKMIARADLER"
     gene            complement(7833..8450)
                     /locus_tag="VF182_05440"
     CDS             complement(7833..8450)
                     /locus_tag="VF182_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEX6436553.1"
                     /translation="MDFKTTLVFDRHLRSSLRRRADRALFKLFALVRFNASAAVGEVF
                     MYLRTLLILSVLGLIAVFSALNWGAFIAPTALSLGFTSVEAPLGLILLAIVGLLTLLF
                     LIYITYLQSTVLLESRRHGRELQSQRDLAEQAETSRFNQLQTFVETELQKVSGDMEQS
                     KSLLLARLDQIERDLRSALEQTGNSLAAYIGELEDRLDRSLGKPT"
     gene            complement(8501..9394)
                     /locus_tag="VF182_05445"
     CDS             complement(8501..9394)
                     /locus_tag="VF182_05445"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6436554.1"
                     /translation="MKTLGFIGTGGMGSGMAANLIKAGHRLVIHDLRREQCGSLESQG
                     AVFKESPKALAESCDIVMSMLPYNEAVQAVGLGNGGLHQAGGGAKLWIDFSSIDKKTI
                     VDVSKQLAQKGWTVLDGSAGGVEEAAAAGTLSLWISGSKDLFDEHQPVFNAMGNKVLY
                     VGDLGNAKLVKNAMAMFAAVQHLSLVEIAAWLKKGGLSEETFRTVLKNSQQNSVAIDR
                     ICEIVASRKFKPRKSWMPKDVGFGLDMARQMEVPMPFVALAYQMFSIAQATGTDGYEA
                     TGIACNVYDLITGRSESELKS"
     gene            complement(9415..9789)
                     /locus_tag="VF182_05450"
     CDS             complement(9415..9789)
                     /locus_tag="VF182_05450"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6436555.1"
                     /translation="MFRASIKHIADIRLVEQGIDRLSWRDFGNGLSMARLAKEGKREL
                     VLYRIAADAAADAFLKHEHVGGEFYLVLKGRIEDETGQFEPGDLVYLDAHSVHTPRAR
                     GDTLVLVLWPEGVELVPEEKIP"
     gene            complement(9936..10511)
                     /locus_tag="VF182_05455"
     CDS             complement(9936..10511)
                     /locus_tag="VF182_05455"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6436556.1"
                     /translation="MDNQQVYLIRHGETEWSLTGQHTGITDLPLTEHGRMLAKRLKPV
                     LAKQKFVMVLTSPLERARDTCELAGLGVEAQVDNDLMEWNYGEYEGLTSREIREKAPG
                     WLLFEHGSPGGETPGQVGVRADRIIARARDQKGNVALFAHGHLLRVIAARWLGLPAGA
                     GRHFLLDTGTLNILGYYQEIPAIKEWNRAIE"
     gene            complement(10670..11089)
                     /locus_tag="VF182_05460"
     CDS             complement(10670..11089)
                     /locus_tag="VF182_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6436557.1"
                     /translation="MPFKRILTAVDFSANSLEAFRVAADMASIHSASLHLLHVIEALP
                     SAGEATVEFVQKANLATEQLVASSQGTLEKVTLTTEVVSGQAFDEIVNRARDWRADLI
                     VLGTKGATSLEQIFLGGTAEHVIKESACSVLIVRPQG"
BASE COUNT         2487 a         3310 c         2913 g         2585 t
ORIGIN      
        1 gacgaccggg actccttttt tgatgatgcc cgccttctcc cgcgcgatcg aagctatatc
       61 gaaaccgaga aaagcttcat gatctctaga gatggtcgtg atcaccgtca cgattgagcg
      121 aactagattg gtggcatcga atcggccgcc aagcccgact tcgatcactg ccgcatcaac
      181 tcgccggcgg gcaaaatata gaaaggccat caccgtcacg atttcaaaaa acgtcagcag
      241 gatatgagct gtctctgttc ggcgccaaat ttcctcggcg agcgcgacaa cttcatcggc
      301 ggtaatttct tcgtcgtcga tgcggatgcg ttcggtgaat gaaaccaaat gcggcgaggt
      361 atacagcgcg gtgcgataac cggcggagca gaggattcga tgcagtatcg cggcagtcga
      421 tcctttaccg ttggtgccgg cgatgtggaa agaaggaaat gttttctccg gatgatcgaa
      481 cagcgccagc gcccgttcca tacgctccag gcgcaaatcg atgatgccgc cgcgcaagtt
      541 ataaattcga ttgagcgttt tcaggtaggc atccatatac tcgcctgtga gcgggtgcct
      601 gaacgaaccg ggttctagtg ttttcgacca ttccggcgcg atcttttcat cgcccgctta
      661 atcttcgaca caaaatctcc ggcagcttgg gctttggcca agctgccgtt agctcgttcg
      721 atggattcaa ccagcgcgct gccaatcacc acggcatcgg cgaattgaac gatccatccg
      781 gcctgctgcg gagtggagat gccgaaacca acaccgactg gaatcgccgt catccgtcgc
      841 acttgggcga cctgagaacg caactgctgg tccagggatt ggcgcgctcc agtgactccg
      901 gtcacagata catagtaaat aaaaccgcga ccctttcgag ctacaagctc taatcgctcc
      961 ttgccgctgg ccggagacaa gagaaaaatt aaatccattc cttcggcgtc cgtccaacgc
     1021 tttaattctt cgctctcttc cggcggcaga tcgacacata aaacgccgtc ggctccggct
     1081 tgcgcagact ggcgcgaaaa ctcttttaag ccgaatctaa aaaatggatt aaagtaacca
     1141 aagagaatca ccggaatctc cgaggtggag cggaatcgcc ggagcatctg cagaatctcg
     1201 gggagagcaa ttttattttt caacgcgcgt tcggaggcgc gttggatggt tgggccgtcc
     1261 gcagtcggat cggaaaaggg aattcccaat tcgatgcaat ccgctccagc cgtttcaagg
     1321 gcacgcatta tctggagcgt ggtctggaga tccggatctc cggcggtgat aaacggaatc
     1381 agtgccgctt cgtcggcctt ttttaactcg gagaattttt tttctattcg actcattctg
     1441 aatcaagttg ttcgctaagc tgaaaattga caggacggct attcggcggc gtgaagtagg
     1501 atctgcgagt acctagaatt tgactcccag gtaatctccc acggtgccca tatctttatc
     1561 gcccctgccg gataaattga ctacaatcgt cttattctta cccagccgcg gcgccagttg
     1621 catcacggcg gcgatggcat gagcgctttc cagcgcggga atgattccct cgacttgagc
     1681 caaaaattgg atcgcttcca tcgcggctcg gtcggttgcc gacgcaaact ccacccggcc
     1741 cagatccttt agatagctca gctcggggcc gacaccggga taatccaatc cggcggcaat
     1801 ggaatgggtc tcgcgtattt gaccgccatt atcttgcaag atgtaactct tgctgccatg
     1861 taagacaccc acctcaccac ccaggactga ggcggcatgc ttgccgctcc tcagacctcg
     1921 tccggctgcc tcgacaccga tcatgcgcac ggttggatct tgaacgaacg ggtaaaacaa
     1981 tcccatggag ttgctgccgc cgccgacgca cgcaactagg caatccggca gcctgccttc
     2041 ctgcctcagg atctgccggc gcgcttcttg gccgatcacc gactgaaaat cgcgcaccat
     2101 cattggatag ggatgagggc cggccaccga accgatcaag taataggtgt caaaaacatt
     2161 ggttacccaa tcgcgcaagg cctcgttcat cgcgtctttc aaggtcttgc tgccggattc
     2221 gacgacgcga acctgagcgc caagaagctt catccgaaaa acattcagcg actgtcgcgc
     2281 tacgtcctcc ttgcccatga aaatttcaca ttgcatgccg aatcgcgcgg ctaccgttgc
     2341 cgtcgcgacg ccgtgctgcc cggcgccggt ttccgcgatg atgcgggcct ttttcatccg
     2401 cctggccagc agtatctggc caatggtatt gttgatctta tgagcgccgg tgtgacaaag
     2461 atcctcccgt ttcaaataaa tcttcgcccc gcccaattgc tcggtaagcc gctcagcaaa
     2521 gtagagcgga gtttcccgac cggcgtactc ccgcaggtga cggtgaaaca tttttcgaaa
     2581 cgcggagtct cgtcgcgcct tcgcgtaagc ccgttccagc tccatgagag ccggcatcag
     2641 ggtttcggcg acgtagcgcc ctccataagg gccgaaatgg ccgtgctggt caggcagttt
     2701 ctttagcggc gctgatgaag cgtttgattt tttcatgatc cttaacaccg gggcgcacct
     2761 ctacgccgct gcacacgtcc acgccgtagg gatgaagccg tcgaaccgcc tcggcgacgt
     2821 tatcgagatc gagcccgccc gacagaatga tttttccagt atcgaatcct tccagccact
     2881 cccagggaaa tgacatgcca gagccgccgt atccagagga ccaggagtcg agaaggtaat
     2941 aatctgcagg gaaatcgtga atacctttca aagattgttt atctttgagc cgaaaagcct
     3001 tgatcacttg aatgccccag ccccggcagt catcggcact ctcatcccca tggaactgga
     3061 ttgtctgata cccgcaacgc ccctcaggga gcgtgccctt cgccaaaatt tgcttgatcc
     3121 gttctttggc ctcgttgacg aacagggcga cattccatgc gtccgcaggc aattggcgca
     3181 aaatggcgcc ggccttctcc ggagcaagag agcgcgagct tggcggatag aaattaaatc
     3241 ccagcgcgtc ggcgccatat tccaacgctt tctcggcatc cgccaagttt gtaatgccgc
     3301 aaacttttac cttaaccatg atggcccgat cgcgccgccg cctaagtcat agacaaatag
     3361 gttgaagctg ccttgtgcgt ggaaatttag gatcgcagca actcttggag cttctcaccc
     3421 gggttcggcg cgcgcatgag cgattcgccg atgaggaaag aatggatgcc ccaccgttca
     3481 acttgcctta tttgttcggc gccgtcgata ccgctctcag agattatcag agtaccgccg
     3541 ggaactcgcg gcgccagcct ttccgtcgtc gccaagctga cttcaaacgt cttcagattc
     3601 cgattgttga taccaattat ttccgcgccg gcgtcgagcg cgcgctccag ctcttcctcg
     3661 ttgtgaactt cgaccacgct gtcgagcgat aaagcggtcg ccgtttcacg cagatcgcgc
     3721 agaaccgttg actctagcaa tgcggcaatc agcaagattg cgtcggcgcc ataacttcgc
     3781 gcctcggtga gctggtaagg atcgataatg aaatcttttc gtaacagcgg cacagcgacg
     3841 gcgcctttga tctcttccag gtatgtcaga ctgccttgaa aaaatttctc ctccgtgagc
     3901 acggaaatgg cgctggcgcc atgatcggca taatctctgg cgataaccac cggattgaaa
     3961 tcggcccgga tcaaaccttt cgacggcgac gatcgtttga tttcggcaac gatgcgccgg
     4021 ctaccgccac tcagctctcg ggcaaagcct ctggtcgctc ggtagtacaa gggtcgatca
     4081 tgcaaagccg ccagcggagt ttgcttttgc cgctgttcca gcacagtgcg cacgtgctcg
     4141 acgatgttgg cgagaaagtc agccataggc ctaggccgcg ttggtcaatt gcgccaattg
     4201 ctcgagcttt tgccgcgctc ttcccgaatc gatggaatct gcggcaaccc ggattccgtg
     4261 ttcgagtgtc tcggctttgc cgctgacata caaggcggcg ccgctgttga gcagcaccac
     4321 atcacgcgcc ggccccttat caccttgcag aatgcgcttg atgattacgg cgctctccgt
     4381 ggcacttccg gcgtggagag cctccaaagg ataaatcttg aaacctatct tctcgggttc
     4441 gaccgtatac tctttgacct ctccatggcg taactcggcg atttttgtcg ggccgcaaag
     4501 cgatatctca tccaacccct ccatgccgtg gaccaccatg gcgcgcgcgc tgcccagatt
     4561 tcttaatacg tgggcgatta ccgagacgag ctcgccgtcg tagacgccga tcagctggtg
     4621 attggccatg gctgggttgg ttagcgggcc gagcaaatta aacaccgttc gtatgccgat
     4681 gtcgcggcgc ggttgcaccg catacttcat cgcctcatgc agcaacggcg caaacaaaaa
     4741 accaatgcca atctgttgga tacagctctc gacgcgctct cggggggcgt cgactctaac
     4801 tcccaaagcg gcaagcacat cggcgcttcc cgactgactc gacaccgaac gattgccatg
     4861 tttggcgacc tcgactccgg cgcccgcggc gacaaacgcc gaagccgtcg atatattgaa
     4921 agtgcctttt tgatccccac cggtgccaca agtatctaag acaaccttcg aaccgacttg
     4981 aacacgatgg gccttttcgc gcatcacccg cgccgcgccg gtaatttctt ctacggtctc
     5041 gcctttcata cggagcgcgg tgagaaagga tgccacctgc aacggcgtcg cctcacccgt
     5101 catgatttga tttatgacat cgatggtttc ctcttccgaa agattgttgc gcctgaccaa
     5161 tctctcgatc gcttctcgaa tattcataat tccctggatc tttagtcatc tgctcgtggc
     5221 aaacgccttg gtcaagtgtt cctttctttg caaagcctcc tgtgttttct cggcctcgtc
     5281 acgtgattcg aaacggccca cgcgcactcg ataccagact cttccctgaa tacgtgattc
     5341 agataaatag gcgttgtagc ctttatccct gagcctatca acccaggttt tggcggattt
     5401 ttcatcagga aatgcattga cttgaacagt ccagggtcgg tgagattctt tggcatcggc
     5461 ttcagcgggc cgagatttgc tgacagccgc cgcaggcttg tcctcagctc gcttggcgat
     5521 ggatgccacc tcttgcctgg ccggcaatct ttctctggct tcagctttgg agtttttctc
     5581 ggctttcttg gcctctcgcg gttcttcttc gttgcgagct tgcgccggcg ccggccttgt
     5641 cagggtgtca tagaatgtta tttcttcttt tggaccggaa aacgctgaac tggtcccctg
     5701 cgcgggcctg atagggattt tcataaccgg ttcggccggt ttgactattt tccgttcttc
     5761 cattccctgt cccaccaaca tgcccagaaa gaaaacgatc atggaagcaa cgccgaagcc
     5821 gcagcccagc aagatgaact gcatgcggct gaagtagaag cgtttgtcct ttcccttgcg
     5881 attctctgcc atagatgccg atcctttggt tgttaggtgg cgctggtcac cgttcgaggt
     5941 cagctcgagc aatcatcttc atcggagcgt cgatgcccag tagggtcaga ccgcgacgga
     6001 cggtcaattg aaccatttgc gccaacaaca tgcgtgctgc ggtcaactct cgatcttctg
     6061 agaggattcg agtgcgatta tagtagcgat gaaattctcc agctagatcc aatagaaaaa
     6121 ataccatgcg gtgcggctcg agctctcgga cgcactcttc gagaacatcg ttgaattgag
     6181 ccatccggcg gatcaactcg atctcctcag cgagttccag ccgctccaac gcgattgtcc
     6241 cgatgtcggc cgcgtccaag aaaattccgt tccggtgcgc ctgttcgaat atactcgcga
     6301 cacgggcgtg agcatactgg acataaaaaa ccggattgtc gctcgactgc tgcttggcga
     6361 gatccaagtc gaaatcgaga tggctatcgg tcttgcgcat gagaaaaaag aagcgcgccg
     6421 catctcgtcc tacttcgtcc agaacttcct gcaaagcaac gaattctccg ctgcgcttgc
     6481 ccattctcac cggctcgccg gctctggtga gttgaaccat ctgcaccagg accacgcgca
     6541 agatgctgtc atcgtagcca agcccgcgca aagacgcctt caagcgcggc acatagccgt
     6601 gatggtcggc gccccagaca ttgatgagtt tgccaaagcc gcgctcgagt ttgttgcggt
     6661 ggtaagcgat gtctgcggcg aaatacgtga gctcgccatc gctcttgatc accgtccggt
     6721 ccttgtcatc gccaaacgcc gtggactgga accattcagc gccgtccttg gaatacacca
     6781 agccgcggtc gcgcagcagc tcgatcgact cagcgatttc gccccgctca cgcatcgctt
     6841 tttcgctgaa aaaagaatcg aagcggatat gaaaatcgct caactgccgt cggatcgtag
     6901 cgagcagaga atcgccgcca tagcggcgaa aaaatgctac cgccgcttct tggctctggc
     6961 tgagaaactt atcgccgtgt tgctttttta tttcggcggc aatgtctttg acatactctc
     7021 cagggtagcc gccttcaggg agttcgcatg aatcgccgaa cagttcgcga taacgagcgt
     7081 acaccgaccg tccgagattc tccatctggt tgccggcgtc attgacataa tactcccgct
     7141 cgacattgaa gccggtcgcc tcgtgcaacc gagccaggac atcgccgatt accgcgaccc
     7201 tgccatgacc gacgtgtaac ggaccggttg gattgacgct ggcaaactcg atctggacct
     7261 tcttcccatg tccaagatca agctgtgtgt ccttgccagt caccagatcg cgcaagcgtt
     7321 gataataaaa cttctgcgaa aaggtaaaat tcaagaaacc cggaccggca atgtcgatgc
     7381 gcgcgagcat gccttcggga tcttcgatat tcttgacaat ggcatccgcg atcactctcg
     7441 gcggtttctt cgcggccttt gcccagtgca tcgcaatgtt gctcgccaga tcgccgaact
     7501 cccgttgttt tggcagctca agaactaaag ccgggatttc tgtcgaactc agatctcccg
     7561 ccttcgatat cttctcgatg gctcgggtta ataaacccgc aaggtgtttt cgcatcggtc
     7621 gtttgatgga gtgattttct tccgctcagg cgatgcacgc cgcaggcgat gacttggcac
     7681 ggctcaatta tacggggaaa gtgatcggaa agcagcgtta taacgatcat ttataacggc
     7741 ttattcgtct tcaagtcaac gacaaccgag ctcaccggcg cgccagacac ctgtagttcg
     7801 accgcgcccg acgtctgggc ggacttattt agctatgtcg gcttgcccaa agatctatcc
     7861 aacctgtcct caagttctcc aatgtaggcc gccaagctgt tgccggtctg ttctaaagct
     7921 gagcgcaggt cgcgctcgat ctgatccaat ctggctaaca gcagagattt tgattgctcc
     7981 atatcacccg acaccttctg tagctcagtc tccacgaagg tttgtaattg attaaatcgg
     8041 gaggtttcag cttgctcggc caagtcacgc tgggactgca gttcgcggcc gtggcggcgg
     8101 ctctccagca gaacggttga ttgcaaatag gtgatgtaaa tcagaaagag aagagttagc
     8161 agccccacga tcgccaataa aatcagtcca agaggcgctt caaccgaggt aaatcctaaa
     8221 gaaagcgccg ttggcgctat aaaagcaccc caattgagcg ccgaaaaaac cgctatcaga
     8281 cccaagacag acagtatcag caatgtgcgc aaatacatga agacctcccc cacggctgcg
     8341 ctcgcgttaa atcgcacaag cgcgaatagc ttgaacaatg ctcgatcggc acgacgacga
     8401 agagaggacc tgaggtggcg atcaaagacg agggtcgtct tgaaatccac gagggacagc
     8461 gcggccagtc cccgaaaccc ggcaattagc gccgcgggtt ctacgacttc aactcagatt
     8521 cgctcctgcc ggtgataaga tcgtacacgt tgcacgcgat tcccgtcgct tcgtagccat
     8581 ccgtcccggt cgcttgagca atcgagaaca tctgataggc caaggcgaca aatggcatgg
     8641 gaacttccat ctgacgcgcc atgtccaatc cgaatcccac gtccttgggc atccaggatt
     8701 tgcgcggctt aaattttcgg ctggcaacga tctcgcaaat gcggtcgatc gcgacggaat
     8761 tttgctgcga atttttgagc acggtgcgaa aagtctcttc cgaaagcccg cccttcttta
     8821 gccaggcggc aatttccacc agactcaaat gctgcactgc ggcaaacatc gccatggcat
     8881 tcttcaccag tttggcattg ccgaggtcgc cgacgtacag aaccttgtta cccatggcat
     8941 tgaataccgg ctgatgttca tcaaacaaat ctttagaacc ggaaatccac aatgacagag
     9001 ttcctgccgc cgccgcttct tcgaccccgc ccgccgagcc atcgagaacc gtccaaccct
     9061 tttgcgccaa ctgcttactc acatcgacaa tcgttttttt gtcgatgctg ctgaagtcga
     9121 tccagagctt ggcgccgccg cctgcctgat gcagcccgcc gttgcctaag cccaccgcct
     9181 ggaccgcctc gttgtatggc agcatcgaca taacgatgtc gcaggattcg gcaagcgctt
     9241 tgggtgattc cttgaacacc gctccctggc tctccaagct gccgcattgc tcgcggcgta
     9301 aatcatggat caccaaccgg tgtcccgcct tgatcaggtt ggcggccata ccactgccca
     9361 tcccgccggt tccgataaac cctagagttt tcatttaacc tcccaacagc aaactcacgg
     9421 aatcttctct tcaggaacga gttccactcc ttcgggccat agcaccaaaa ctaaagtatc
     9481 gccgcgagcc cgtggcgtat gaaccgaatg cgcgtccagg tagacgagat cgcccggctc
     9541 aaactggccg gtttcatcct cgatacggcc cttcaatacg agataaaatt cgccgcccac
     9601 gtgctcgtgc ttgaggaaag cgtccgcggc ggcatcggcg gcgattcgat aaagcaccag
     9661 ctcgcgtttg ccctccttag ccaatcgcgc catcgaaagg ccattaccga agtcacgcca
     9721 ggataaacga tcgatcccct gctcaactaa acggatatcg gcgatgtgtt ttatcgatgc
     9781 tcgaaaaatt gccgtacctc tattccttct gggctaaata acacgcagtt tgcgcgaacg
     9841 aaagcacttt gtccgatcgt gatgcgtcgc cagtgggcga tttagattag gcgatagtct
     9901 cgttgggaaa tcggccgggc aacgcgcggc ttccactatt caattgcgcg gttccattcc
     9961 ttgatcgccg ggatttcttg ataataacca agaatgttca gggtgccggt atcaagaaga
    10021 aaatgacgcc cggcgcccgc aggcaatccc agccagcgcg ccgcgatcac ccgcagcaag
    10081 tgcccatgcg cgaagagcgc gacgtttcct ttctgatcac gcgctcgcgc gatgatgcga
    10141 tcggcacgca cgcccacctg gccgggagtt tcgccgcctg gagagccgtg ctcaaagagc
    10201 aaccatcctg gtgccttttc gcggatctca cgcgacgtca atccttcgta ttcgccgtaa
    10261 ttccactcca tcagatcgtt atcgacctgc gcctcaacgc ccagcccggc gagttcacag
    10321 gtgtctctgg cgcgctcaag ggggctggtt agaaccatga caaacttctg tttggctaga
    10381 accggcttca gccgcttcgc cagcatccgg ccatgctccg tcagcggaag atcggtaatt
    10441 ccggtgtgct ggcccgtgag gctccactct gtttcgccat gccggattag atagacctgc
    10501 tggttgtcca tactaatttg cctcgcgtcg acatatcctc tttacgttcg caccggtttc
    10561 atgctgcctt gaagccggcg cgaggaagac taaagcgcaa ttcgatggat cagattattt
    10621 tccatgagcg atgattcgac agcatcagcg tctagccgta ctctacgcgc tatccttgcg
    10681 gtcgcacgat taacaccgaa caagcagatt ccttgatcac gtgttcggcg gtgccgccta
    10741 aaaagatttg ctcgagcgac gttgctcctt tggtccccaa cacgatcagg tccgcgcgcc
    10801 agtcgcgagc gcgattgacg atctcgtcga aggcttgccc gctgacaact tcggtagtta
    10861 gtgtcacctt ctccagagtc ccctgtgatg acgcgactaa ctgttcggtg gcaagattgg
    10921 ctttctgaac gaactctacg gttgcttccc ccgccgacgg taacgcttca atcacgtgaa
    10981 gcaaatgcaa cgaagccgag tgaatcgagg ccatgtcggc ggcaacgcga aaagcctcta
    11041 gtgagttggc agagaaatcg acggcagtta agattcgctt aaatggcatg atcggcctcc
    11101 cggtttgggc tttaacattc tagtttactt taaacgaaaa cacctggtgg aagtaagcac
    11161 gtgcgcgatt actgcggcct gctgtctatc ggcaagaaag ttcgctggac tgatttacgg
    11221 agcgatccag cgcagacgga aacttattgg ttctccaaga cctggggtta ttcgattcga
    11281 agacgccgcc gggca
//
LOCUS       DASUVS010000099        15500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_469830, whole genome shotgun sequence.
ACCESSION   DASUVS010000099 DASUVS010000000
VERSION     DASUVS010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 15500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15500
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469830"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VF182_05465"
     CDS             complement(<1..737)
                     /locus_tag="VF182_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436558.1"
                     /translation="MNVILNIAFSSRAVLLLLGLLLTGRSSATATPLKAGTVIESMGI
                     GSKQRLSEFPVTVHVILQIHEHPDPKGREIQDMGKVAACQESIVSTVLSIAENGVPAV
                     IVEGFYFAGTFDKPASIAVSELPRSDRMQSKWILASQKNLFVYGFEVKFLNDLAGWVV
                     KELGKSADKAVELGKEPRSTDRDTNKIDVAKLLQEEVTRLNLWHTGIIPERSFLALQT
                     ALAVALARQERQVQLVIGKLHWADLVYA"
     gene            1042..1812
                     /locus_tag="VF182_05470"
     CDS             1042..1812
                     /locus_tag="VF182_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436559.1"
                     /translation="MAQEDLRETLGLEQVVELREKTEKISRSLLGQLRDHLDTLRPLL
                     APARVFGRHVRSQSREDIPDAETALRKLCEKYNGLCAKPFSLPPNLETDAVADLDGRL
                     ELYPWEYTHEAKDQNQTKTITVTSPVRHVLTYRSSYSLSQIRLAVSGKGDRRQDDVRE
                     FLVAALAMQSALDKFPGLVRLFEELRYHVQVEKNPALGDLPLVVISSSLPSFRPSDSV
                     VLATTRLSGVPAFIELVDPKAIQQIRDPLKSRLEEVLR"
     gene            complement(2009..2854)
                     /gene="ybgF"
                     /locus_tag="VF182_05475"
     CDS             complement(2009..2854)
                     /gene="ybgF"
                     /locus_tag="VF182_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEX6436560.1"
                     /translation="MYLKSPRRFKRFRAGLIVTVNLALVSASACVQPERVEVLEREQR
                     RIQSDNVSQQKEIDATRANLADTRANLQQAQREISSLKELIEETRHQVGRQIDTTSRE
                     GDQRVKDLEAKLGKLAEGFKSQGEQLKQREDELKEVRKGLQLPAEAAEMASAENETIR
                     RDYEVAWRNLEKRDYRVAIARFKDFQKKYPKSNLAGNAQYWIGEGHYALREFDQAIIE
                     FDAVRTKYPEAAKIPAALLKQAYAFAELGENTNARLLLQEVVEKYAQTPEAIQAKQKL
                     KSLES"
     gene            complement(2866..3498)
                     /gene="pal"
                     /locus_tag="VF182_05480"
     CDS             complement(2866..3498)
                     /gene="pal"
                     /locus_tag="VF182_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX6436561.1"
                     /translation="MFYNKIASYSFLSAAILSAAACGSSSAKPDVVSGGTVATEASDS
                     QISGEGRSDKTRESSLPSSTVNQPKADQAPAASSSSLQQLKEGKSTTTAASSPLRDVL
                     FEFDRHDLTPAARDILVSNAEWLKKNASTRIEVEGHCDERGTSEYNLALAAKRAQATR
                     DYLLTLGIAPDRISTISYGEEIPACIESNESCWRQNRRVRFVVAPPRSAS"
     gene            complement(3631..4476)
                     /locus_tag="VF182_05485"
     CDS             complement(3631..4476)
                     /locus_tag="VF182_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436562.1"
                     /translation="MRSVNLWRICFLLFLIACSAFNIQGQFTSGRQALITGDAATAVA
                     RFQRVAEEDPNYQVGSVAFRQSIWTYLGRAQYSLGNLPEAKESLEKALAQSSEDHLAR
                     LYLGLILLRQPSAPVPNNPFTLKDVSYALTEGVAPKRVMALVSERGIDFDLTKEAEAQ
                     LKRSGADATLLDQLRKSAAENDMRRKTAGNPAQGAKHISSAVTALRTDLESVVHTSSQ
                     GKFWDPSGEIRKQMDAALLLLSSREADTAKIISTGEWVGLRLEEEIDNARRDESQQRR
                     NEQRR"
     gene            complement(4545..4982)
                     /locus_tag="VF182_05490"
     CDS             complement(4545..4982)
                     /locus_tag="VF182_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436563.1"
                     /translation="MKPEAMTKAEQKASAAGKTDISQEKVVTLPDQLKETADQVVTLE
                     NIAIRDGDVSGELVNRSTQGVYGVDLQVLYSWRWNNDFHPGRHDPGRAEYFKIEKEIA
                     PGQRVPFVHKPSPPLPARKDGTFDISIKVIGFTRIFREKAVAR"
     gene            5939..6838
                     /locus_tag="VF182_05495"
     CDS             5939..6838
                     /locus_tag="VF182_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX6436564.1"
                     /translation="MAIRMGTSEYGGTFYTQGTAFAELFNRGRGGEDKCIVQTTDASI
                     HNAEQLDRGELEFGFMASNWIGRAKKGTAPFKREIDLRMVAPANVGPMFFVKRADSSI
                     QSVAEFKGKRIAIGTKGSGMEQHVHTIFGVLGIEFDSFTPLNMSFVQGADELIAGNID
                     AQFQPPIPNRVMTDLSLRADVRVIPYAPGQLPRVVGEVPFYRSVIIEKDAFRGVVEDI
                     AQLGVVNVLVTHARTSESIVRDMARSIADNLDNLPIMNRLFTGLKNLFEPLRIQGRAA
                     LEFGGVPLHPGAIRAYREAGWLS"
     gene            7007..8446
                     /locus_tag="VF182_05500"
     CDS             7007..8446
                     /locus_tag="VF182_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6436565.1"
                     /translation="MERNLIFIAGATGYIGGRLLIVLEAEGYRVRCLARRPEYLQSRI
                     GPATQVVAGDVFAPETLDAALAGVDQAYYLVHSMGSAESFEQQDRIAARNFAASARRA
                     GVRRIIYLGGLGTGDALSSHLRSRQEVGNILRDSGVPVIELRASIVIGSGSLSFEMIR
                     ALVERLPVMITPRWVSVTAQPIAVRDVLDYLAGALKLATTESRIFEIGGKDRVSYKDI
                     MAEYARQRGLRRVMISVPVITPRLSSLWLALITPVYARTGRKLVDSIRNPTIVRDPAA
                     LTAFNIRPLGMVAAIAAALRNEDLEFAATRWSDSLSAAGAQPTWAGVRFGNRLIDTRV
                     ADVNAPSEQVFAVICRLGGARGWYYANWLWQLRGWCDLLVGGVGLRRGRRHSEELRVG
                     DALDFWRVEAVDVNRRLRLLAEMKLPGRAWLEYLLEPHGTGTRITQNAIFDPVGLAGL
                     AYWYAVYPLHRNLFGGMLRAICRKAEQSL"
     gene            8570..9448
                     /locus_tag="VF182_05505"
     CDS             8570..9448
                     /locus_tag="VF182_05505"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX6436566.1"
                     /translation="MKERSQVALRVAPDNKLANDWALVLISQGLSPSLRHDEGGVVLS
                     VPAEEVELALAGLAAYEKENTAPVQKVQNRSDDFSNFVTGLVVAGVLILSHAATVKWK
                     EAAPWLERGGAEASQILDGELWRTVTALTLHADLVHLFSNAVAAAIFVTALSGLVGAG
                     VAVALFVVTGACGNLANAFIQGSAHIAIGASTAIFGAVGVLGTVGLVRKRREPGRRRS
                     AWIPVAAALGLLAMLGTGTGRTDVLAHLFGFLCGAVLGVPVALFFPAPPGRLTQWTCG
                     GAAAGLLIYCWSLALD"
     gene            complement(9502..10785)
                     /locus_tag="VF182_05510"
     CDS             complement(9502..10785)
                     /locus_tag="VF182_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016862688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6436567.1"
                     /translation="MRIQLNNLKFLVRALSSRNFRLFFFGQAVSLIGTWMQQIAMRWL
                     VYRLTKSELMLGVVGFVSDIPLFLLVPFAGVLADRFRRHRILFVTQMLAAIQAAVLAV
                     LVITESVAVWHVVILGGSLGIISAFDITARQAFIVDMIENKDDLPNAIALNSFIFNGA
                     LLIGPAVAGILIAAVGEGLCFAINSVSYLTVIGALIMMKIPKKKFSSSALGLRAAIIE
                     GATYTFASVPIRSILMFVAVVSFVAASYPLLMPVYAETTLQGGPRVFGYLMSATGVGA
                     LAGAIFLASRNSLQGLGEMIVIAAILYGVGYFALAMSHVLPISLIIALVLGFSLMMQM
                     ASSNTIVQTIVEDQKRGRVLSLFVMARRGIESLGSLLFGAIAAWIGTPGTMMIAGTVC
                     LLAVAVFATQLPSIRQAGNSFSENSDRARSRKTKL"
     gene            11037..11300
                     /locus_tag="VF182_05515"
     CDS             11037..11300
                     /locus_tag="VF182_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX6436568.1"
                     /translation="MDKHRMNPDQFKCDWLEFKGELQKNWKDFSEEDLTEIEGDYDKF
                     VTMVQKRCRDKEEDVVRWANDWYSRREQHEAAARHSTISRNQM"
     gene            complement(11473..12468)
                     /locus_tag="VF182_05520"
     CDS             complement(11473..12468)
                     /locus_tag="VF182_05520"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6436569.1"
                     /translation="MKTCAIDIHHHYVPQSLLEEARKRGKHLGVELTEKDGQQSLSFA
                     GGPPFVLHPELPAIDARLKMMEDSKLAMAALEAHTATLGYRLTGEQGENWCTAYNEGI
                     KGLIDSHPNHFVGMASVPLQDPARAAKVLEHAVRDLKFKGGYIGTNVNGNYYGTADFD
                     PFWAKAQELDVMIVMHPEDVAGADKMNPYGLKLICGNPADSALCFGYLTYSGVFDRFP
                     DLKLCILHGGGFFPYHLGRFDRGWQVRAGARAAKAKMPPSAYLKNIYYDDMIYRLDTI
                     KYLIGLAGVDHIMVGTDYPYDLGDWDAAKKVEELDISEADKLLMLEGNAKRLLKL"
     gene            complement(12581..14245)
                     /locus_tag="VF182_05525"
     CDS             complement(12581..14245)
                     /locus_tag="VF182_05525"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6436570.1"
                     /translation="MTHDLLIKNGTVVDGSGSPAYQADVAIDGEKIAAIGKNLGSGKK
                     ELDAKGQVVAPGFIDSHTHMDAFVVQYPNGNPVVNYGVTSIVIGDCGASCAPVPPKPE
                     PRKVLVQYLRRVLDKYVDDKDWKWNTFAEYLNYLEGKIAVNVLPLMPHSPVRLTVMGE
                     AAYARESTPEELDTMKKMVREGMELGAIGFSTSPRGGPAIHAGTPSTFATHQEIIELA
                     NVAAEYHGCFQFNGFQMVLQPESGVPEMLTKIKTLQIGNEFRVRPGAKAAGMQSIKYM
                     EEAQKRGQDIYGVVIPYQHIRRFNVDDCFLFNGLPTWEAIKNSSDLKSKLADKETRRK
                     IEQERVACEGKLEFPEWHGWDRVVFEHVKKPELKKLEGKNVAEIARLTEKAPVDAFFD
                     TWLEDNLDSQILYHGLANAHPELLAEMIKSDTSLIGTDVGAHLDRFFWHGAPTKVLGF
                     WRREKNLFSLEEAVHKLTGFPAKKLRLNRGLVKEGMPADLTVFDPDKIDDLVSKQLPD
                     IIDAEEVKRHPPGMKAVIVNGATVVEEGQCNDVFPGKVRRQQLCIN"
     gene            14476..14994
                     /locus_tag="VF182_05530"
     CDS             14476..14994
                     /locus_tag="VF182_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436571.1"
                     /translation="MEPNYEDAKRKVSAWVEKHKQAVLLDEESSTILDVASGRKTVIP
                     WRDLAAFEEKIHPETQDCYLVLLFDDGKQIALVDPGGVAFAPSLENTGPLRDAPPVVC
                     LKDFHTLKPRIDHYLYDHPAEPPPKETLDLVMLCIAILDGARAVGFDIADLEGELEKS
                     LGDIERRTGPKN"
     gene            15049..>15500
                     /locus_tag="VF182_05535"
     CDS             15049..>15500
                     /locus_tag="VF182_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX6436572.1"
                     /translation="MGIVNNLHCPKCAYQAASRTEFQCPQCRCILEVDVEIGHLTRAD
                     FEAMRQSRDRSIWRWFDFFPVEDRSCIVSLGEGSTPLIHSRRLAESAGLANLYLKNDT
                     VLPTGSLKDRSNSVGIAKARELGFSTATVMSTGNAAASVAAYAAAADM"
BASE COUNT         3414 a         4145 c         4211 g         3730 t
ORIGIN      
        1 gcatagacca aatcggccca atgtagcttg cctatgacga gctggacctg cctctcctgc
       61 cgcgcgagtg cgacggcgag tgctgtttgc agagccagaa agcttcgttc cgggataata
      121 cctgtgtgcc agaggttcaa acgcgttacc tcttcctgga gcagcttggc aacgtctatt
      181 ttatttgtat ccctatctgt actcctgggc tcttttccga gttcaaccgc tttgtcggca
      241 gatttgccca gctcttttac gacccagcct gccaaatcat ttaaaaactt cacttcaaaa
      301 ccataaacga acaggttttt ttgactggcc aaaatccatt tcgactgcat ccgatcgctg
      361 cggggcagct ctgaaaccgc gatagatgcg ggtttatcaa acgttccagc aaaatagaat
      421 ccttcaacga tcacagcggg aacgccgttc tcggcgatgc taaggacggt ggaaacgatg
      481 ctttcctgac acgcagcaac cttgcccatg tcttgaattt cgcgcccttt tggatcggga
      541 tgttcatgga tctgaagaat tacatggacg gtcactggga attcactcaa ccgttgcttc
      601 gagccgatac ccatggactc aatcactgtc cctgctttca gaggcgttgc ggtggcgctg
      661 gagcgtccgg tcagaagcaa acctaaaaga aggagtacgg ctctggaact gaaggctata
      721 ttgaggatca cgttcataaa acctcccgag gcgtcatcgc ctaaacgcgt gtgtagagat
      781 acgccgcaga gtggagtaca gcattcgggc gacctcttgc caagacgtta caaatccgag
      841 aaatgtcaga acataaaact ccgcaaggct cgacggcgcg ggtggacgtt tgatctctaa
      901 tgcgccggaa tgatcttacc tcgtccgaat ccacgatgaa tctgcccgca gatgatctga
      961 ttcggtggcg tatttcgctg atgtgcaact cgcattaagt gcttttcttg actgggtaac
     1021 tgtggtacat gccaagctgt catggcacag gaagacctac gcgaaacatt ggggctcgaa
     1081 caggttgtcg agcttcgcga aaaaaccgag aagatttcta gatcgttgct gggccagcta
     1141 agagatcatt tggacacact ccggcctctg ttagcgcctg cgcgtgtttt tggcaggcat
     1201 gtccggtcac aatcgcgaga ggatattccc gacgcagaga ctgccctgag aaagttgtgc
     1261 gagaagtata atggactctg tgccaaacct ttttccttgc cccccaactt ggagactgat
     1321 gcggtagcgg atttggacgg tcgcctcgaa ctttatccat gggaatatac ccatgaagcg
     1381 aaggatcaaa atcaaaccaa gacgatcacg gtaacctcac ccgtgcggca cgtgctgacg
     1441 tacaggtcct cttactcgct gtcgcagatc aggttggcgg tatctggaaa aggagatcgt
     1501 cgacaagacg atgtccgaga attcttagtg gccgcattgg cgatgcaatc cgctcttgat
     1561 aagttcccgg gcctcgtccg gcttttcgaa gaattgcgct accatgttca agtcgagaaa
     1621 aatccggctc tcggcgacct cccgctcgtc gtaattagct cgtctcttcc ttccttccgt
     1681 ccctcggatt cagtggttct ggccacgacc cgattatcgg gtgtgccggc gtttatcgaa
     1741 ctggtcgatc ctaaggctat tcagcagata cgcgacccgt taaaatcccg tttagaggaa
     1801 gtgctacgtt agcatccgtc cggcgcggcc tgttgatctc acgttctgct ccgatgatga
     1861 aagttagcta gcgaggccct cttcttcggt aaagcaccga ccattgaatg cttcgcacgg
     1921 gctgagtgga accggaaacg aaatcagagc gaattacgtt attgagtttg attggagagg
     1981 atacggagga acaaggttta atgcgttgtc atgactccag tgacttcaat ttctgctttg
     2041 cctggattgc ctcaggtgtt tgcgcgtact tctcaacgac ttcttggagg agtaatcttg
     2101 cattggtatt ctcgcccagc tcggcgaagg cataggcttg cttaagcagc gctgctggaa
     2161 tttttgctgc ttcgggatat ttcgtgcgca ctgcgtcaaa ttcaataatc gcctgatcga
     2221 actctcttag agcataatga ccttcgccga tccagtattg agcgttaccg gcaaggttgc
     2281 ttttggggta ctttttttga aagtctttga aacgcgcgat ggcgactcgg taatctcgct
     2341 tttctaaatt tcgccaagca acttcgtagt ctctcctaat cgtttcattt tccgcagagg
     2401 ccatctcagc tgcctctgcc ggcaactgta atcctttacg cacttctttt aactcgtctt
     2461 cacgctgctt cagctgttct ccctgagatt taaagccttc tgctagcttg ccaagtttcg
     2521 cctcgagatc ctttacccgc tgatcgccct cgcggctggt agtgtcgatc tgccggccga
     2581 cctggtggcg agtttcttca atcaattctt tcaaactgct gatttctctt tgtgcctgct
     2641 gcagattggc gcgggtatcc gcaaggttgg ctcgggtcgc atcgatctcc ttttgctgac
     2701 ttacattatc actctggatg cgccgctgtt ctcgctcgag cacctccacc cgttccggtt
     2761 ggacgcaggc ggaagcactt acaagtgcaa ggttcacggt gacgataaga ccggctctaa
     2821 atcgcttgaa ccggcgagga gattttaaat acatacggac cttcttcaag aagcagagcg
     2881 aggcggagcc acgacaaaac gaactcgccg attctgccgc caacagctct cattgctttc
     2941 tatgcaagcc gggatctctt caccgtaact gattgtcgat atgcgatcgg gtgctattcc
     3001 aagggttaga aggtaatctc gagtggcttg ggctcgcttg gctgccaggg ccaaattgta
     3061 ttcactggtt ccccgttcgt cacagtggcc ttccacctct atcctggtcg atgcattttt
     3121 cttcagccat tcagcattac tcacaagaat gtcacgcgcc gccggcgtga ggtcgtgccg
     3181 atcaaattca aacagcacat ccctgagagg acttgatgcc gcggtcgtgg tggatttgcc
     3241 ctctttcaat tgttgcaagc tggaagagga cgctgccggt gcttgatcag ctttcggctg
     3301 attaacagtg gatgacggca aactcgattc tcgcgttttg tcactccgac cttcacctga
     3361 aatctgagaa tcagatgcct cggtagcaac tgtgccgccg gagacaacgt caggtttggc
     3421 gctggacgaa ccacaagccg cggctgagag gatagccgcg ctcaagaagc tgtagctggc
     3481 gatcttgttg tagaacattc tatctccgtt acatgaactc aaagaagtta ttctacgagg
     3541 agggatagcc gccgatttag catctgcgtc aaaaactgtc aatgtatgat tttacttgtc
     3601 gttaacggca tagtcagacg aatcaccgga tcaacgcctt tgctcgttgc gccgctgttg
     3661 gctttcgtca cggcgtgcgt tgtcgatctc ttcctccagc cgtagaccta cccactcgcc
     3721 agtagaaatg attttggccg tatccgcttc acggctggag agaaggagaa gagccgcgtc
     3781 catctgcttg cggatttctc cactcgggtc ccaaaatttc ccctgggagc tagtatgaac
     3841 gaccgattct aaatcagtcc ggagcgcggt cacagccgag ctgatgtgct tcgcgccttg
     3901 cgccggattt cccgcggtct tccgcctcat gtcgttctct gccgcactct ttcttaattg
     3961 atccaacagt gttgcatcgg ctccactgcg ttttagctgg gcctctgctt ctttggtgag
     4021 atcgaagtca ataccgcgtt cgctcacaag ggccatcact cttttaggtg caacgccctc
     4081 ggttagcgca tatgaaacat ctttgagcgt aaaagggtta ttggggaccg gcgcgcttgg
     4141 ttgccgcagc agaatcaaac cgagataaag cctcgccaga tggtcttcgc tcgactgcgc
     4201 gagggccttt tctagactct ctttggcttc cggcaaatta cctaagctgt attgggctcg
     4261 gccgagatat gtccaaatac tttgtcggaa agccaccgat cccacttggt aattcggatc
     4321 ttcttctgcg acacgctgaa atcgagccac tgcggtagcc gcatcgccag taataagcgc
     4381 ttgtcggccg gacgtgaatt gtccctgtat attgaaggca ctgcaggcga tcaaaaagag
     4441 cagaaagcag atgcgccaga gattaacgct tctcatctgt caatatcgcc attaccgact
     4501 cgactcacca tggcagcacc gcgcttgcgg ggtttgcaca cagatcatct cgcgacggcc
     4561 ttttcgcgaa agatcctggt aaagccaatg actttgatac tgatgtcgaa ggtgccgtcc
     4621 tttctcgcag gcagaggcgg cgacggctta tgcacaaacg gaactcgttg gccgggagct
     4681 atctcttttt cgatcttaaa atactcggct ctgccgggat catggcgccc gggatggaaa
     4741 tcgttgttcc accgccatga gtagagaacc tgcagatcca ctccgtagac gccctgagtc
     4801 gatctattca ccagttcacc ggaaacgtcc ccatctctaa tcgcgatgtt ttcgagagtg
     4861 acgacctgat ctgctgtctc ttttaactga tcaggaaggg tcaccacttt ttcctgagaa
     4921 atgtctgtct ttccagccgc gctcgctttt tgctcagctt ttgtcatcgc ttctggtttc
     4981 atttgtgctt cgtcgctctt gaccacttgt ttttcggtcg ttggttcagc cttggtcgcg
     5041 ggtttagccg tctcggctgg tttgcgctga tcctttgctg atgcgtcgct ctttgttttt
     5101 gcggcttcga gtttaagtct acggcgaata gccgctagat tgagcgagtc aattactgta
     5161 ggccctgctc ctatcttact gagccttgcc cttacatcat ctgtgagctc gaaatctatc
     5221 ccacgctctt tgaccagctc tgcgattcgc cgttgagcga catctccgct tagcaaagtc
     5281 tctagggcag attgcgataa ttcttgggct aatccaggcg aagagaagcc aaggacaatg
     5341 aacaaaccag ctagtatgga cagctttaca atcgttaccg ggcggtaatt gcctagcgat
     5401 cgtttcatac ttctaggggc gaaaggttgt cgacccggaa tgatctgatg ctgcggcgag
     5461 ctgcgcaatc gaaaacaggg tgttgtcgac atcacatcga gagttcactt gctgaaaatg
     5521 atttattcct ccgaagcatt ctataggtcc ttttcagatt cagcggtata cttccattac
     5581 tagattctca atgtctgtcc atagcatagc ggagcctacc ggggcaaatt gttgcacgca
     5641 gtgtttgcga acacgacgac tcacttaaac ctgatttgct gacacagaag atcacgttat
     5701 tcgcttagta gcgtcccgta ctgcccatta tggagggtcg atggattgaa gctactcgga
     5761 catcactgcc agcggcacct agcaaaagcc tttgcgcggg taactcgctc ctaaatcgga
     5821 attgtcgaaa atacgagaaa aaattttcgg ttctactgaa atttgcagct atcgcttggg
     5881 atagtgtaag agtaaatcaa agtatctatc cagattggaa ggatgagcgg gcaattctat
     5941 ggcaatacgc atgggtacgt cggaatacgg cggcacgttt tatacgcagg ggaccgcatt
     6001 cgcggagttg ttcaatcgcg gcaggggcgg cgaagacaag tgcatcgtgc aaacaaccga
     6061 tgccagcatc cacaatgcgg agcaactgga cagaggcgaa ctggaatttg gtttcatggc
     6121 ttcgaattgg atcgggcgag ccaaaaaggg gacggcgcca tttaaacgtg agatcgacct
     6181 gcgcatggtt gcgccggcga atgttgggcc gatgttcttt gtcaagcgcg ccgactcgtc
     6241 gattcaaagc gtcgctgagt tcaagggtaa gcgcatcgct atcggcacta agggcagcgg
     6301 catggaacaa cacgtgcaca cgattttcgg tgtgctcgga atcgagttcg acagcttcac
     6361 gccattgaac atgagttttg tccaaggcgc cgatgaattg atcgccggca atatcgatgc
     6421 gcagtttcag ccgcccattc cgaatcgcgt tatgaccgat ctgagcctgc gcgccgacgt
     6481 tcgcgtcatc ccgtatgcgc cgggtcaact cccaagggtc gtcggcgaag tgccatttta
     6541 ccgaagtgtc atcatcgaaa aggatgcatt tcggggagtt gtggaagata tcgcgcaact
     6601 cggggtggtt aatgtcctag tgacacatgc tcggacttcg gaaagcatcg ttcgcgatat
     6661 ggcgcgaagc atcgcggata atctggataa tctgccgatc atgaatcgcc tcttcacagg
     6721 cctcaaaaac ctgttcgagc cactccggat tcagggtcgc gcggcacttg aattcggcgg
     6781 ggtgccgttg cacccgggag cgatacgagc ctacagagaa gcaggctggt tgagctagca
     6841 gcccacggca aaatcgattc ctcgctagac gcagcattta tcgaaatccg ccgggttgcc
     6901 ggcgatccta catgatcctc cgtcgaatcc gatatcctat cgacttggaa gccagcgaca
     6961 tctttgtgag attaacagca cagccgatag aagctcagca taggagatgg agcgaaatct
     7021 catttttata gccggggcaa ccggatacat cggtggccgt ctgctgatcg ttctggaagc
     7081 agaaggctac cgagtccgct gcctcgcgcg tcgtcctgag tatctccaat cccgaattgg
     7141 ccctgccacc caagtggttg ccggggatgt ctttgcccct gaaactctcg acgccgcttt
     7201 ggccggagtg gatcaggcct actatctcgt tcattccatg ggaagcgctg agtctttcga
     7261 gcaacaggac cggatcgcgg cgcgcaattt tgccgcttcc gcgcggcgcg ctggagtacg
     7321 tcgaatcata tacctcggtg gtttgggaac cggtgacgcg ctttcctctc atttgcgtag
     7381 ccgtcaagaa gtcggcaaca tcctgcgcga ctctggagta cccgtcatcg aattgcgcgc
     7441 ctccatcgtt atcggctcgg gtagcctctc ctttgaaatg attcgcgctc tggtcgaacg
     7501 gctgccggtc atgattacgc cgagatgggt ttcggttacg gcgcaaccga tcgcggtgcg
     7561 cgatgtgctg gactatctgg ccggcgctct gaagttggcc acgaccgaaa gtcgtatctt
     7621 cgaaatcggc ggaaaggatc gagtgtccta caaggacatc atggcggagt atgcccggca
     7681 acgcggtttg cggcgcgtta tgatttccgt tcccgtaatc actcccaggc tatcgagtct
     7741 gtggctggcg ctgatcaccc cggtttatgc gcgaactgga cgaaagctcg tcgacagtat
     7801 tcgcaatccg acgattgtcc gagacccggc agcgctcact gcctttaata tccggccgct
     7861 cggtatggtg gcagcgattg cggcggcgtt gcgtaacgaa gacctggaat ttgccgcgac
     7921 ccgctggtcc gattcgctct ctgcagcagg ggcgcaacct acctgggccg gtgtacgatt
     7981 cggcaataga ctgatcgaca cgcgggttgc cgacgtcaat gctcctagcg aacaggtctt
     8041 tgccgtgatt tgtcgactcg gtggagcgag gggttggtat tatgcaaatt ggctctggca
     8101 gttacgcggc tggtgcgact tgctggtcgg cggcgtgggc ctgcgccgcg gtcgccggca
     8161 ctcggaagag ttgcgggtcg gcgatgctct ggatttttgg cgggtggaag cagtagatgt
     8221 caatcgccgt ttgcgcttgc ttgcggaaat gaaattgccc ggccgcgcct ggctcgaata
     8281 tctattggaa ccgcatggca ctggaactcg gataacacag aatgcgatct tcgatcccgt
     8341 gggcttggcg gggcttgcgt attggtatgc tgtctatcct ctgcatcgaa acctattcgg
     8401 cggcatgctg cgtgccattt gccgtaaggc cgaacaaagt ctatagcgaa tgcgcgctct
     8461 tctccggcct cattaaaaat cttttaagtg aaaaccgctc gcgcgtgtcg atggcgcaca
     8521 tgaaaagctc ggatgtcacc atttgctgcc aagttatgat gcaactctca tgaaggagcg
     8581 ctcgcaagta gctttaagag ttgctcctga taataaactg gccaatgact gggcgttggt
     8641 tcttataagc cagggtttat cgcccagttt acggcatgac gaagggggcg tggttctgag
     8701 cgttcctgca gaagaggtgg agttggcgct cgccggcctg gcagcgtacg aaaaggagaa
     8761 tacggctcct gttcaaaaag ttcagaatcg ttcggatgat ttttcgaatt ttgtcacagg
     8821 gctcgtggtt gcgggtgtgc tgatcttgag ccatgccgct accgttaaat ggaaagaagc
     8881 cgcaccttgg ctcgagcgag gcggcgccga ggcgagccaa atacttgacg gcgagctgtg
     8941 gcggacggtg actgccctta cgttgcacgc tgatcttgtc catttgttta gcaacgctgt
     9001 ggcggcagcc attttcgtta ccgcgctctc cggtctggta ggcgccggcg tcgcagtcgc
     9061 tctgttcgtt gtcactgggg cttgcgggaa tttagctaat gccttcattc aaggttctgc
     9121 ccacatcgcg atcggagcct ctaccgccat ctttggcgca gtcggggtgc tcgggaccgt
     9181 gggattggtt aggaagcgga gagaaccagg gcgaagacgt agcgcctgga ttccagtggc
     9241 ggcggcactg ggcttactcg cgatgctcgg caccggcacc ggacgcacgg atgttctggc
     9301 tcatctgttc gggtttttgt gcggcgctgt attgggagtt ccggtcgcgc tgttctttcc
     9361 cgctccgccg ggacggctga cgcaatggac ctgcggcggc gccgcagccg ggttgctgat
     9421 ctactgctgg agccttgctc tggattgaga aactcgaatg gaataatcga aattcaaatc
     9481 gtcccgggcg agcgtctcgt cctagagttt tgtcttgcga gaccttgctc gatcgctatt
     9541 ctcactgaag gaattgcccg cttgccggat ggagggaagc tgggtggcaa aaaccgcgac
     9601 tgcgagaagg cagacagtgc cggcgatcat catggtaccg ggcgttccga tccatgccgc
     9661 aatcgcgccg aatagcaggc tccctagtga ctcgatgccg cggcgcgcca tgacgaacag
     9721 gcttagcacc cggccacgtt tctgatcctc gacgatagtc tgcacgatgg tgttgctcga
     9781 tgccatttgc atcatcaacg agaagcctag cacgagcgcg ataatcaaag agatcgggag
     9841 cacatgggac atcgctagcg caaaatatcc gacaccgtag agaatcgccg cgatgacgat
     9901 catctctcca aggccttgga ggctgttgcg cgaagcaaga aatatcgcgc cggcaagcgc
     9961 gccaacgccg gtagccgaca tgagataacc aaaaacacgt gggccgccct gcagagtagt
    10021 ttctgcgtag acaggcatga gcagcgggta cgatgcggcg acgaaactca ccacggcgac
    10081 gaacatcagg atcgagcgaa tcggcaccga tgcgaatgtg taagttgcgc cttcgatgat
    10141 tgcggcccta agaccgagcg cgctggaaga gaacttcttt ttagggatct tcatcatgat
    10201 aagcgcgccg ataacggtca ggtaacttac gctgtttatc gcaaagcaca gcccctctcc
    10261 cacggctgcg atcagaatgc cagccactgc aggaccaatt aaaagcgcgc cgttgaagat
    10321 aaaagagttc agagcgatgg cgttgggcaa gtcatcctta ttctcgatca tatcaacgat
    10381 gaaggcttga cgcgcggtga tatcaaaggc gctgataatg ccaaggctcc cgcccaggat
    10441 aacgacatgc cagacggcaa ccgattcggt gatgacgaga acagccagta ccgcggcctg
    10501 aatcgctgcc agcatttggg tgacgaagag tattcgatga cgtctgaacc gatccgccaa
    10561 tacaccggcg aacggtacta aaagaaacaa cgggatgtcg ctgacaaatc ccaccactcc
    10621 caacatcaac tccgatttgg tgagccgata gaccagccag cgcatcgcga tctgttgcat
    10681 ccaagtaccg ataagcgaca ccgcctgacc aaaaaagaac aggcggaaat ttcgcgagct
    10741 cagcgcgcgc acgaggaact tgagattgtt taactggatg cgcatcaatc taagacgcag
    10801 gtcatgaatc tatgtttagc gaaaacggcc gtggcgagaa acccgtcgag ctttcttgat
    10861 ttagatacaa tcacacactc gatcgagaat gtccctggcg cgggactttt gtttcaaaca
    10921 cgacacatca caaacttaac ctgaagcaaa aacttaaaaa taacttctca ttttctcgaa
    10981 tccggcttgg cacctgctgt gcaccttacg gaagaaatcg caagattgga ggttgtatgg
    11041 acaaacatcg gatgaatccg gaccaattta aatgtgactg gcttgaattc aaaggcgaac
    11101 tgcaaaaaaa ctggaaggac ttctcggaag aagacctgac cgaaatcgaa ggagattacg
    11161 acaagttcgt gacgatggtg cagaaaaggt gccgtgataa ggaagaagac gttgtccggt
    11221 gggccaacga ttggtactcc agacgtgaac aacacgaagc cgcagccaga cactccacga
    11281 tatcacggaa tcaaatgtaa caatgttaaa cttcgatcgg cgacttgcgg attgcactag
    11341 cggcgagcgg tgtctacact cccgacgccg ctagtgtccg tgtcgcgaaa agcttcttag
    11401 ctgatctggc cgaagcgtcg aaaatctagt cagcaacttg cggctgtacc tgtaaacccg
    11461 gcaaatcggt aactacaact ttagcaagcg tttggcgttt ccctcgagca tcaacagctt
    11521 gtcggcttcg gagatgtcca gttcctcaac cttcttcgcg gcatcccagt cgccgagatc
    11581 atagggatag tccgtgccga ccatgatgtg gtcgacgcca gccagaccta tcaaatactt
    11641 gatggtatcg agccgataaa tcatatcgtc gtagtagata ttcttcagat aggcgctggg
    11701 cggcatcttg gctttggctg cgcgcgcgcc ggcccgaact tgccagcccc ggtcgaagcg
    11761 gccgaggtgg tacgggaaga atccgccgcc gtggagaatg caaagcttga gatcgggaaa
    11821 gcggtcgaag acgccgctgt aggtcagata gccgaagcac aacgcgctgt ccgcgggatt
    11881 gccgcaaatc aatttcaaac catagggatt catcttatcg gcgccggcga cgtcctccgg
    11941 atgcatcacg atcatcacat cgagctcctg cgctttggcc cagaacgggt cgaaatccgc
    12001 ggtgccgtag tagttgccgt tgacgttggt gccgatgtag ccgcccttga atttcagatc
    12061 acgcacggcg tgctccagga ctttggcggc gcgcgccggg tcttgtagcg gtaccgaagc
    12121 cataccgaca aagtgattgg ggtggctatc gatcaaacct ttgatgccct cgttgtaggc
    12181 ggtgcaccag ttttcgcctt gctcgccagt gaggcgatat cccaaggttg ctgtgtgagc
    12241 ttccaacgcg gccatcgcaa gcttcgaatc ttccatcatc ttaagccgtg catcgatggc
    12301 cggaagttcg gggtgaagca cgaaaggcgg cccgccggca aacgacaacg actgctgccc
    12361 gtctttttcc gtcagttcga caccgagatg cttgccgcgc tttctggctt cctccaacag
    12421 gctttggggt acgtaatgat gatggatatc gatagcgcag gttttcattt tgattggtcc
    12481 tttatgtgaa actaagatcc aattgaatgt ggcgctggcc gccaagccaa cgccacatga
    12541 tcgaggaaga aacgataaag cgggatttcc tacttactgc ttaattgatg caaagctgct
    12601 gacgccggac tttacccggg aagacgtcgt tgcattgacc ttcttccaca accgtcgcgc
    12661 cattgacgat gaccgccttc atccccggcg gatgtctctt cacttcctcg gcatcgatga
    12721 tgtcgggcaa ctgcttgctc actagatcgt cgattttatc cggatcgaaa acagtcaagt
    12781 ctgcaggcat tccttctttg accaacccac ggttgagccg aagtttcttc gccggaaaac
    12841 cggtgagctt gtggaccgct tcttctaggc taaacaggtt tttctcgcgc cgccagaagc
    12901 cgagaacctt ggtcggcgcg ccgtgccaga agaatcgatc gagatgcgct ccgacgtcgg
    12961 tgccgatcag gctggtgtcg gatttgatca tttcggccag cagctcggga tgagcgttgg
    13021 ccagcccatg atagaggatt tgcgaatcca ggttgtcctc caaccaggta tcgaagaacg
    13081 cgtcgaccgg cgctttttcg gtcaagcggg cgatctccgc gacgttcttg ccttcgagct
    13141 ttttcagctc cggctttttg acgtgctcga atacgacccg atcccaacca tgccattcgg
    13201 gaaattcgag tttgccctcg caagcgaccc gctcctgttc gattttccgt ctggtttctt
    13261 tgtcggccag cttggatttt aaatcgctcg aattcttaat cgcctcccaa gtcggcaggc
    13321 cgttgaaaag gaagcagtcg tcgacgttga agcggcggat gtgctggtag ggtatcacca
    13381 cgccgtagat atcctggccg cgtttctgag cttcttccat gtatttgatc gactgcatgc
    13441 ccgcggcttt tgcgccgggt cgcacacgga actcgttgcc gatttgtaat gttttgattt
    13501 tcgtcaacat ttccggaacg ccggattccg gttgcagcac catctgaaag ccattgaact
    13561 gaaagcagcc gtggtattcg gcggcgacgt tggccagctc gatgatctct tgatgcgtcg
    13621 cgaatgtgct cggcgtgccc gcgtgaatcg cggggccgcc gcgcgggctg gtggagaagc
    13681 cgatggcgcc cagctccatg ccttcgcgca ccattttctt cattgtgtcc aattcttccg
    13741 gagtggattc gcgcgcgtag gccgcttccc ccataaccgt caagcgaacc ggagaatggg
    13801 gcatcagcgg cagcacgtta accgcgatct tgccttcgag atagttcaag tattcggcga
    13861 aagtgttcca cttccagtcc ttatcatcga catatttgtc caacacccga cgtagatact
    13921 gaaccaaaac cttgcgtggt tcgggtttag gtggcacggg cgcacacgaa gcgccgcaat
    13981 cgccgatgac gatgctggta acaccatagt taaccaccgg attgccgtta ggatattgca
    14041 cgacaaacgc gtccatatgg gtatgagagt cgatgaatcc cggcgccacg acttgtcctt
    14101 tggcgtcaag ttcctttttt cccgaaccta aatttttgcc gatagcggca atcttctcac
    14161 cgtcgatagc cacgtccgct tgataggccg gacttccgct gccgtcgacc accgtgccgt
    14221 tcttgatcag taagtcatgc gtcatatgcg tgtagcctcc tttgtgaact tatttcgctt
    14281 atcccaccga aggtcacagg ctgtcaacca ctcggaatga gcttcgagct tgtaagcagc
    14341 cgttccggct gttccaaccg ttccaataat ttcagtcgtt cgaatcatct ggaactgggc
    14401 caagccctgg aacgtttgga accattggaa ccaggaaggg acgagagcga aatctttttg
    14461 tgctacagta ggtccatgga gcccaattac gaagacgcca aacggaaagt gagcgcctgg
    14521 gtggaaaagc acaagcaggc tgtgctgtta gacgaggaga gttcgacgat cttggacgtt
    14581 gcatccgggc gaaagacggt tatcccttgg cgtgacctgg cggccttcga agaaaaaatt
    14641 catcccgaga cgcaagactg ctatctggtt ctgctcttcg atgacggcaa acagatcgcg
    14701 ctggtggatc cgggaggtgt ggcatttgct ccatcattgg agaacaccgg gccgctacgg
    14761 gatgctccgc cggtggtttg tctcaaagac tttcacacgc tcaaaccgcg catcgaccat
    14821 tacctctatg atcatcccgc tgaacctccg ccgaaggaga ctctcgattt ggtgatgctc
    14881 tgcatcgcca ttctcgacgg cgcgcgcgcg gtgggattcg atattgccga tctggaaggc
    14941 gaattagaga agtctctggg cgacatcgag cgccggacgg gtccaaagaa ttgaagtatt
    15001 gagcttcggc tggcgagatc ttttctaatt ttcaggtaaa aaatccatat gggaattgtt
    15061 aacaatctgc attgccccaa gtgcgcgtac caggcagcgt ctcgaaccga gttccaatgc
    15121 ccgcaatgcc gatgcattct cgaagtcgac gtcgagatcg gtcatctgac gcgtgccgat
    15181 tttgaagcga tgcgccagag ccgcgaccga agtatctggc gctggttcga cttttttccg
    15241 gtcgaagata gatcttgcat cgtctcgctg ggcgagggaa gcacaccgct aattcactcg
    15301 cgtcgtctgg ccgagagcgc cgggctggcc aatctctatt taaagaatga caccgttcta
    15361 ccaaccggat cgctcaagga ccgcagcaac agcgtcggca ttgccaaagc gagagaactg
    15421 ggctttagca ctgcgaccgt gatgtctacg ggtaacgcgg ctgcttcggt agcggcgtat
    15481 gccgccgccg ccgacatgca
//
LOCUS       DASUVS010000100        11510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1344877, whole genome shotgun sequence.
ACCESSION   DASUVS010000100 DASUVS010000000
VERSION     DASUVS010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11510
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344877"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VF182_05540"
     CDS             complement(<1..899)
                     /locus_tag="VF182_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6436573.1"
                     /translation="MRELNYYEAKFEALDEIMAGDQRVHLINGTFLSLSPHRHVYQNL
                     LQKYRERMISPPISELGFCGLATGAAMAGLRPIVDLSTGSFIYEAWPQVANEAANAYY
                     MSGGQTRVPVVFHIFHGLRGGGAAQHSGSPQAMLWNVPGLEIVLPASPRDVKGLLKTA
                     VSTDNPTIFVDHVKLLDVRGDVDQDQKPIPFGVADIKRKGGDVTVIATSYAVQSCLTV
                     ADRLAKENIEVEVIDPRTLVPFDLATILSSIEKTHRVVLVDETHQSCGVAAEIAARIA
                     ESGFDKLKTRIRRVSTLDVPVPF"
     gene            complement(908..1927)
                     /locus_tag="VF182_05545"
     CDS             complement(908..1927)
                     /locus_tag="VF182_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX6436574.1"
                     /translation="MLPKLAREALLDLCRRMFTIRHFEESLVPLHDRGVFSGHYHLYI
                     GQEATGVPALSCLRADDYLFTTHRNHGHLLARGADPKRLCAEILGRVDGLNRGKGGSF
                     HSVAPSLGFLHSSGVVAGIIPLSTGTAYAAKALQNGRITVCLFGDGALEEGAAPEAFN
                     LASLYKLPVLFLCENNGKYGSGRATAAAQSSSMAAYPLTDLPAAYKVPAQQIDGLDSG
                     LVHATISEAVEKIRRGEGPQFVEAQTVRWPGSETNWPAVVQPTDSSLAWDVSNVPEKV
                     REWYRTCDPLLIYVRELVDAKHASRQEIAGMEQQVKSEIAAAVELAVASPYPKVEEAE
                     RDFFA"
     gene            2036..3031
                     /locus_tag="VF182_05550"
     CDS             2036..3031
                     /locus_tag="VF182_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436575.1"
                     /translation="MARHRRLMLLSMFIGCFFLTSSLFAQTKSLKKIHVGVPAISMGN
                     MIIFFTKEAKLFEKHGLDADVVVMSGSGISSRALVGGSVEISPIATPTVMLSVVAGSD
                     MVILAHTMPGVIQSLMVRPEIKKDEDLKGKIIGVTTFGSLTDFLVRHLAKKKGMNPDK
                     DFALLQTGGDAERLIALQQGKIHAATLSHPVFILAQRAGFVPLWDFFKEVDYPWSEIV
                     SRRSTIQRDRDLIMRYMRAHIEGIALFKKDPDLAKRVIKKTLRLDDDALAQESWEVFA
                     KYRLAAPYPNIKGLKTSYEYVGATRPDVYKYKPEEFVDASFVEELDKSGFIKKLY"
     gene            3047..3985
                     /locus_tag="VF182_05555"
     CDS             3047..3985
                     /locus_tag="VF182_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6436576.1"
                     /translation="MKVRIGITLGQSLGSVKRPEEILDFVDHLEALNVDSLWVSDRLV
                     SPALTLEPITFLSYVAGRLRRMKFGTSTLVLSTRNPVILAKQLATLDFISQGRLFPAV
                     GLGSDDAQDFAAVGISKKERGQRSDEAIVLMRRLWSEENVSFSGKFFSIQDVTITPRP
                     WQKGGPPIWIGGRSQAALRRTGRLGDGWLVSGASAAEVGDGIKAIRAYAAEAGRVVPE
                     DHYGVLLPFYFARTADEAYDAAKKSLRLRPELSAYDFTALGRPEQVRRRIAQYIDAGA
                     SKFVMRPCGPFEKWHGQIEMLASEVIQPLQTPDEKP"
     gene            complement(4183..5178)
                     /locus_tag="VF182_05560"
     CDS             complement(4183..5178)
                     /locus_tag="VF182_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015145806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX6436577.1"
                     /translation="MRAMLFEKARQPLRWADVPPPQPDAGQVLIRVRACAVCRTDLHI
                     IDGELEQPQLPLIPGHEIIGVVEKIGKEVHRLKPGDRIGVPWLGWTCGRCEFCLSARE
                     NLCDQARFTGYTLDGGYGEYAVADPRFCFAIPDFYSDAEAAPLMCAGLIGYRSLVKAG
                     EGKRLGIYGFGAAAHIIAQVAAFQNREIYAFSRPGDEAAKNFALGLGAVWAGSSSELP
                     PVKLDAAIIFAPVGKLVPQALRALKKGGIVVCGGIHMSDLPSFPYSILWQERSVCSIA
                     NLTRRDGEEFLPLAALVPVRTQVETFALEEANEALDRLRSGNITGAAVLVTDHSR"
     gene            5423..6469
                     /locus_tag="VF182_05565"
     CDS             5423..6469
                     /locus_tag="VF182_05565"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX6436578.1"
                     /translation="MKLLLVALGVLMILATVIPFLKKDEWWVRIFDFPRLQITLIIAL
                     VFAAYLLLRGNSIADYGFLVGLILCLTYQSYMIYPYTRLSPKQVQWSKNAAKDSSFSL
                     LFANVLIENGNAAGLKEIIKKADPDLILTAETDAWWREQLKEFDRTHPYVVAQPQDNC
                     YGILLQSRLELIIPQIKFLVQDDIPSIHTRVKLRSGIEVELRGLHPRPPVPEEDGRST
                     ERDAELLVVGKEVKGKKDPIVVLGDLNDVAWSRTNNLFQDISGLLDPRIGRGFYHSFH
                     AKYPFIRFPLDHFFHSNHFRLMELRRLPYFGSDHFPVYIKLNYEPDAEKEQEELHADQ
                     SQKQEAEEKIDQVR"
     gene            complement(6597..6905)
                     /locus_tag="VF182_05570"
     CDS             complement(6597..6905)
                     /locus_tag="VF182_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436579.1"
                     /translation="MAEKESAIITVTCPCCSATLTIDPVLSAVLDHKLPPKPQLLDHL
                     KDAVGYVKAEATKRDEKYQQVAAAEKNKSKVLEAKFQELFKKAKEEPITKPIKDIDLD
                     "
     gene            complement(7031..7399)
                     /locus_tag="VF182_05575"
     CDS             complement(7031..7399)
                     /locus_tag="VF182_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6436580.1"
                     /translation="MFKRIDHVAIHVKDLGRSVEFYQKHFGFKHYFQHAATGGMQIAY
                     LKLGDTVLELTHKSDGPMSGFHFCLETDNFDQTVAALQKNGVELVRAPHDTAAREARE
                     EGWRRVVFAGPDGEQIELRG"
     gene            7572..8258
                     /gene="aat"
                     /locus_tag="VF182_05580"
     CDS             7572..8258
                     /gene="aat"
                     /locus_tag="VF182_05580"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX6436581.1"
                     /translation="MPIFRLVDEPIFPPPDYADPSGLLAVGGDLSGERLLEAYRLGIF
                     PWYSDDQPILWWSPDPRFVLHLEEFHISRSLRKTLRQGIFQITIDRAFDEVIAACAAV
                     PRLGQNGTWITAEMQEAYSELHGLGYAHSVETWFEGKLAGGIYGVSLGKAFFGESMFH
                     RQSNASKVALAALVEKLKSWNFLFLDAQMTTEHMMSLGAREVSRRIFLKQLGLALKHP
                     TRRGKWRIKD"
     gene            complement(8255..8791)
                     /locus_tag="VF182_05585"
     CDS             complement(8255..8791)
                     /locus_tag="VF182_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436582.1"
                     /translation="MIRPEFKTILLDQRGAAVIIWCFFVISIAVYVFISRHLLANPAF
                     GRGFSYAIAARAVFWLLAFIDIGYYIYWKRRLAPEVVLREAKTSKRLRALEGYKGGEE
                     ERAAAVVSTYVTRKVVLFAIIEAIAVYGLILALVGRFLADQYLLSALALILLIVEFPS
                     VKSLSAVLHNLETTAQSS"
     gene            9020..10612
                     /locus_tag="VF182_05590"
     CDS             9020..10612
                     /locus_tag="VF182_05590"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX6436583.1"
                     /translation="MAEFDIVIHGGQVIDGTGNPWFYADVGIRDGKIAGIGKIDPEAG
                     QRAISAKGFVVSPGYIDMHTHSDQPVIADGNAESKVRQGVTLDIIGESQTVAPLEGAV
                     LEEYRLEHRKRNGIEADWKTFTDYFSRLMKEGTSINVASGVSPQQVKRVVCGFEERPA
                     SASEQEKMNRLVAQAMEEGALGLTAAWHAKGPESPEEVVEMAKVVKRYGGYYGVHVGS
                     EGFDMMEELAKTLRIARESRIPIHVYHLKMRAKANWGRIRQVIEQIEEARREGLEITA
                     NQYPYTAMQHPWRRLFPRWVQDAPINETISQFRSASFRERVMNDPEFDQYVNEHGGWE
                     GIVAARLDTEQLKRFEGKPIAEIAKIRGQDPVSTCFDMIYEEGVFIHGVHHTMSEEDV
                     KTVMRVPWVSIGSDGSALNLNFPGKPHPRSFGTNPRVLGKYTREEKVLALEDAVRKMT
                     SLPAQVLGLKDRGLLREGYWADVVVFDPDRVADMATYENPKQYPKGIDYVLVNGAIVI
                     DNGHHTGARPGRVVYGPGKMDV"
     gene            10701..11504
                     /locus_tag="VF182_05595"
     CDS             10701..11504
                     /locus_tag="VF182_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6436584.1"
                     /translation="MASLLISDTWERGDPYEQYVGRWSRRVAPQFLSWLNIPARRRWL
                     DVGCGTGALSAAILDQCAPTSIVAVEPSEGFLEKAKEQLGSRVLLRRGSAAEIPLDDR
                     AVDVTISGLVLNFVPDGRAAVSEMARVTAPGGTIAAYVWDYSGKMELMRYFWDAAVEL
                     KPEAAKLDEGVRFPLCHPEALSELFASAGLRRPQVTAIDITTSFAGFEDYWRPFLGGQ
                     GPAPAYAMALDDPERARLAERIRQRLPIQANGSISLIARAWAVRAAAAS"
BASE COUNT         2672 a         3088 c         3235 g         2515 t
ORIGIN      
        1 ctgaagggca ccggcacatc cagagtggat acgcgacgaa tgcgcgtctt caacttatcg
       61 aaaccgctct cggcgatgcg cgcggcaatc tcggcagcga ctccgcaact ctgatgcgtt
      121 tcatcgacga gcacgacgcg atgggttttc tcgatcgagg acaggatcgt tgccaggtcg
      181 aacggcacca aggtgcgtgg atcgatgacc tcgacctcga tgttctcctt ggccagccga
      241 tcggcaactg tgaggcagct ctggaccgca tatgaggtcg cgatcacggt tacgtcgccg
      301 cccttgcgct tgatgtcggc aacgccgaac gggatcggtt tctggtcttg gtcaacatca
      361 ccgcgcacgt ccagaagctt aacatgatcg acaaagatcg tcgggttgtc ggtggaaacg
      421 gccgtcttga gcaggccttt gacatcgcgc ggcgaagccg ggagcacgat ctccaatccc
      481 gggacattcc ataacatcgc ctgtgggctg ccggagtgct gtgcggcgcc gccgccccgg
      541 agtccatgga atatatgaaa gactaccggc actcgagtct ggccccccga catgtaatag
      601 gcattggcgg cttcattggc cacctgcggc cacgcctcat agataaaact tccggtgctc
      661 aggtcgacga tcggccggag cccggccatt gccgctcccg tggccaaccc acagaagcca
      721 agctcggaaa taggcggcga gatcatgcgc tcccgatatt tttgcaggag attctggtag
      781 acgtggcggt gggggctaag gctcaagaac gtgccgttga tcaaatgcac tcgttgatcg
      841 ccggccatga tttcgtccaa tgcctcgaac ttggcttcgt agtagtttag ctctctcata
      901 attatctcta cgcgaagaag tcgcgctctg cctcttcgac tttcggatac ggactggcca
      961 ccgcaagttc gaccgcagcg gcgatttcgg atttgacctg ttgttccatc ccggcgatct
     1021 cttggcggct ggcgtgtttg gcatcgacca gttcacgaac gtaaatcagc aacggatcac
     1081 aggtgcggta ccattcgcgc actttctccg ggacattgct cacatcccat gccaaggagc
     1141 tatcggtagg ctgcaccaca gccggccagt tggtctcgct gcctggccag cgcacagtct
     1201 gcgcctcgac aaactgcggc ccttcgccgc ggcgaatttt ttcgactgcc tcggaaatcg
     1261 ttgcatgcac aagcccggaa tccaggccgt cgatctgctg cgccggaact ttataagccg
     1321 ccggtaagtc ggttaacgga taagccgcca ttgacgagga ctgcgccgcg gctgtcgcac
     1381 gaccgctgcc atacttgccg ttgttctcgc acaggaagag caccggcaat ttgtagagtg
     1441 acgcgagatt gaatgcctcg ggcgccgctc cttcctccaa cgcgccgtcg ccgaacaggc
     1501 aaaccgtaat ccggccgttt tgcagcgcct tggccgcgta agcggtgccc gtcgagagcg
     1561 ggataattcc cgccacgact cccgaactat ggagaaatcc caaagacggc gctaccgagt
     1621 gaaacgaacc gcccttgcct ctgttgaggc cgtcgacgcg tcccaaaatt tcggcgcaca
     1681 gccgtttggg gtcggcgccg cgcgctaata agtggccgtg atttctatgg gtggtgaaga
     1741 gataatcgtc cgctcgcagg caagacagcg ccggcacccc ggttgcctcc tggccgatgt
     1801 acaaatgata atgaccgcta aacacacctc ggtcatgcag cggcaccaga ctttcttcaa
     1861 aatgacggat cgtgaacatc cgccggcaca aatccaacaa cgcctcgcgc gctagtttgg
     1921 gtaacatgat gcctgagata atcaaaaggc cgcgctttgt caacgtcgaa tgattgacat
     1981 cattttgata tcgcgactat atccgaatag atgaagcgct cgggaggaat gatcgatggc
     2041 tcgtcatagg cggctcatgt tgctcagcat gtttataggt tgcttttttc ttacttcatc
     2101 gctttttgct caaacgaaat cgctgaaaaa gattcatgtc ggcgtgccgg cgatcagcat
     2161 gggcaatatg atcatctttt tcaccaaaga ggcaaagctg tttgaaaagc atggactgga
     2221 tgccgatgtc gtggtcatga gcggatcggg gatctcatca cgagcattgg taggcggcag
     2281 tgtggaaata tcgccgatcg cgacgcctac ggtcatgctt tcggtagtgg ctggctccga
     2341 catggtgatt ctcgcccata ccatgccggg tgtgattcag tcgctgatgg tgcggcccga
     2401 gatcaaaaaa gacgaagacc tcaaaggaaa aattattggc gtgacaacct tcggatcgct
     2461 taccgatttt ctcgtgcgcc atcttgccaa aaagaaaggc atgaaccctg acaaggattt
     2521 tgcgttgctg caaaccggcg gcgatgcgga gagactgatc gcgctccagc aaggcaaaat
     2581 ccatgcggcg actctttcgc atccggtatt tattctcgcg caacgagccg gctttgtccc
     2641 gctgtgggac tttttcaagg aggttgacta tccctggagt gagattgtgt cacgaaggtc
     2701 gacgatacag agagaccgtg atctgatcat gcgctatatg agagcgcata tcgagggcat
     2761 cgcattattc aaaaaagatc ctgatttggc caagagagtt attaagaaga cgcttcgact
     2821 agatgacgac gccttggctc aggaatcttg ggaagtgttc gcgaagtacc gcttggccgc
     2881 gccttacccg aatataaaag gcttgaaaac cagttacgaa tacgttggcg cgacccggcc
     2941 tgacgtttac aaatacaagc cggaagagtt cgtcgatgcc agcttcgtcg aagaactcga
     3001 taaaagcgga ttcatcaaaa agctctactg aagatgctcc agcgaaatga aagtcagaat
     3061 agggataacc ctgggacaat ctctagggag cgttaaaaga cccgaggaga ttctcgattt
     3121 tgtcgatcat ctcgaagcct tgaatgtaga ttcgttgtgg gtcagcgatc gcctggtctc
     3181 gcctgccttg acgctcgaac cgattacttt tctatcttat gtggccgggc gcttgcgccg
     3241 gatgaaattc ggcacgagca ccttggtgtt gtccactcgc aatccagtca ttctggccaa
     3301 acagctggcc accctcgact ttatttccca gggacgtttg ttcccggcgg tgggactggg
     3361 cagcgatgac gcccaagatt ttgccgcggt tggcatcagc aaaaaagaac gcgggcagcg
     3421 ttcggatgaa gcgatcgtct tgatgcgccg cctgtggagc gaggaaaatg tcagcttcag
     3481 cggaaaattc ttttccattc aggatgtcac gatcacgccg cgcccgtggc agaaaggcgg
     3541 cccgcccatc tggatcggcg gccgcagcca ggcggcgctg cgccgtaccg gccgattggg
     3601 cgacggctgg ctggtgtccg gcgcttcggc tgccgaagtc ggggatggaa tcaaagcgat
     3661 tcgcgcatac gccgccgaag ccgggcgtgt ggtgcctgaa gatcattacg gtgtattgtt
     3721 gcccttctat tttgcgcgca ctgccgacga agcttatgac gcagccaaaa aatccctgcg
     3781 gttgcgtcct gagctatcgg cgtacgattt cacagcattg ggcaggccgg aacaggtgcg
     3841 gcggaggatc gcacagtata tcgatgccgg cgcaagcaaa tttgtcatgc gcccatgcgg
     3901 gccgttcgaa aaatggcacg ggcagatcga gatgctcgca agcgaagtga tccagccgtt
     3961 gcagactccc gacgaaaaac cctaaagatt atattttccg tctcgccgcg tcttgaaaac
     4021 tctgtttcgc acattgctga aaaacaccgc agtaacgaga cccgtgctct gtggtcgtga
     4081 aaacgtgatc gagaattctg aacgagcacg aacacagcca cgaatacgag cttgagcgat
     4141 ggactgcaac cagtatcagc tcgaaggtcg atccgaaggg gactaacggc tatgatccgt
     4201 caccaggact gccgcgccag taatgtttcc ggatcgcaag cggtcgagag cttcattggc
     4261 ctcttccagc gcaaaggtct caacctgcgt tcgcaccggg accagcgcag caagcggcag
     4321 aaattcttct ccatcacggc gggtcagatt ggcaatcgaa cagacggagc gttcctgcca
     4381 tagaatcgaa tagggaaaag atgggaggtc actcatgtga atgccgccgc agactacaat
     4441 tccgcctttt ttaagcgcgc gcagagcttg cggcaccagc ttcccgaccg gcgcaaagat
     4501 gatcgccgca tcgagcttca ccggcggcag ctcgctggag cttccagccc aaacggcgcc
     4561 gagtccaagc gcgaaattct ttgccgcttc atcgccgggc cggctaaaag cgtagatctc
     4621 gcggttctga aatgctgcca cctgcgcaat gatgtgggcg gcggcgccga agccgtaaat
     4681 gcccagccgc ttgccttccc cggcctttac caaactccgg tagccgatca acccggcgca
     4741 catcagcggc gcggcttccg catcactgta gaaatccgga atggcaaaac aaaaacgcgg
     4801 atcggcgacg gcatactcgc cgtagccgcc gtcgagcgtg tacccggtga agcgcgcctg
     4861 atcacataga ttctcgcgag cggataagca aaactcgcag cggccacagg tccagcccaa
     4921 ccaaggcacg ccgatccggt cgccaggctt caaccgatgg acctctttgc cgattttctc
     4981 gacaacgcca atgatctcat gcccaggaat caaaggcagc tggggctgct ccagttcgcc
     5041 gtcgataata tgtaaatcgg tccggcacac ggcgcaggct cgcacccgga tcaaaacttg
     5101 tccggcgtcc ggttgcggcg gcggcacgtc tgcccagcgc aacggttgcc gggctttctc
     5161 aaaaagcatc gcgcgcataa ggtcaatcta ttcttatcgc tgccagttta gaagcctaca
     5221 gactgcgcag gccaggcatc attaggagcc aagagatcta ccgcgcttga aacttggtcc
     5281 cagcaagcgt acgcacctta gttaacctct actggggctt tctgaaagtt gaataaattt
     5341 cgccagtgaa tattactggc accgcgactg cgttattatt agctacaagc cttagcgacg
     5401 gagcagagcc aggggaaaaa tcgtgaagtt actattggtc gcgctcggtg ttctcatgat
     5461 attggccacc gtgattccgt ttttgaagaa agatgaatgg tgggtcagaa tctttgattt
     5521 cccgcgcttg cagattactt taataatcgc gctggtcttt gccgcgtacc tgctcctcag
     5581 aggcaactcc atcgccgatt atgggttttt agtcggactg atactgtgtc tgacatatca
     5641 aagttacatg atttatccgt atacgcgctt gtcgcccaaa caggtgcaat ggagtaagaa
     5701 cgcggcgaaa gactcgagct tcagcctgct gtttgcaaac gtgctgatcg aaaacggcaa
     5761 cgcagccggc ttgaaagaaa tcatcaaaaa agccgatccg gatcttatct tgaccgcgga
     5821 aaccgacgca tggtggcgag aacagctaaa ggaattcgac cgaacacacc cgtacgttgt
     5881 cgcgcaaccg caagacaact gctatggaat acttctccag tccaggctag agttgataat
     5941 tccgcagatc aaattcctgg ttcaggacga cataccttcc atccatacgc gagtcaaact
     6001 gcgatccgga atagaagtgg agctacgcgg actccatcca cggccgccgg ttccagagga
     6061 ggatgggagg tcaaccgaac gcgatgcgga gctgcttgtg gttggaaagg aagtcaaagg
     6121 aaagaaagat cccatcgtgg ttctgggcga tttaaacgat gtcgcgtggt cgagaaccaa
     6181 taacttattt caggacatta gcggactatt agatccacga atcggccgcg gcttttatca
     6241 ttccttccat gccaaatacc cgttcattcg atttccgtta gatcacttct tccattcgaa
     6301 tcattttcgg ctgatggaac tgcggcggct accgtacttc ggttcagatc attttccggt
     6361 ttatatcaag cttaattacg agccggatgc cgagaaggag caggaagaat tacatgcgga
     6421 tcagtctcag aaacaagagg ccgaggagaa aatcgaccag gtcaggtgag tatctctaag
     6481 tttgccggat cggtcgcttg gagtttgaaa taacctgtga atcgacctca gacaccagct
     6541 cgtctataac agccttcgag cggtcgtaag cgggcaagac accaatcgtc agcagtctag
     6601 tccaaatcga tatctttgat gggttttgtg atcggctctt cttttgcctt cttgaacagc
     6661 tcttgaaatt tggcttccag caccttggat ttatttttct cagcggcggc aacttgttga
     6721 tacttttcat cgcgcttggt cgcctccgcc ttcacgtatc cgacggcgtc cttcagatga
     6781 tccagcaatt gaggtttggg aggtaatttg tgatcgagca cggccgaaag aactggatcg
     6841 atcgtcaatg tcgcactgca acaaggacag gtcacggtta taatcgcgct ctctttctca
     6901 gccattatca atcctccagt cattcatctt gtgcagaagc actccagcgg cgattttgga
     6961 ttggaatttt ggaaatcgga ttacgagttg aaattcaatc gaaaatcggc aatccaaaat
     7021 ctaaaatgcc tcatccccgt aattcgatct gttcgccgtc cggccctgca aaaacaaccc
     7081 ggcgccagcc ttcttctcgc gcttcccgcg cggcagtatc atgcggcgcg cggactaatt
     7141 cgacgccgtt tttctgcaat gctgccaccg tttgatcgaa gttgtcggtt tccaagcaaa
     7201 agtgaaaacc actcatggga ccgtcggact tatgcgtcag ctccaagacg gtatctccca
     7261 attttaggta ggcgatctgc atccctccgg tggcggcatg ctgaaaatag tgcttgaagc
     7321 cgaagtgctt ttggtagaat tcgacggaac gcccgaggtc tttgacgtgg atagctacat
     7381 gatcgatgcg cttgaacata ctgtgctcct tatcggaagg atagtcccgg actgggacgg
     7441 tttgcaagcg agtgaaaaaa tgccggctac gaagtaaaat agcagctata gaaaatggag
     7501 tattggagtg ctgagttata aatgcattaa ttcattactc caccactcca atatttcagt
     7561 cttttgatcc gatgcccatc tttcgacttg tcgacgaacc gatttttccg ccacccgact
     7621 atgccgatcc tagcgggttg cttgcggtag gaggcgattt gtcgggtgag agactgctgg
     7681 aggcttatcg actgggcata ttcccatggt actccgacga tcaaccgatt ctctggtggt
     7741 cgccggaccc gcgctttgtg ctgcatctgg aagaattcca tatctcaaga agcttaagga
     7801 agacgctgcg gcagggtatt ttccagatta cgatagatcg ggctttcgat gaagtgatcg
     7861 ctgcttgcgc tgccgtaccg cggctcggcc agaacggcac ttggatcacc gccgaaatgc
     7921 aggaagctta tagcgagctc catggcctgg gttatgccca ctcggtagag acctggttcg
     7981 aagggaaact agccggtggc atctacgggg tttctctcgg caaagcattc ttcggcgaat
     8041 cgatgtttca ccgccagagc aacgcctcga aagtggcgct ggccgcgctg gtcgaaaaat
     8101 tgaaaagctg gaattttctt tttctggatg cgcagatgac cacggaacac atgatgagtc
     8161 tgggcgcgag agaagtttcc cggcgcattt ttttaaagca gcttggtttg gccctgaagc
     8221 atccgacgag acggggcaag tggcgcatca aagattagct gctttgcgcc gtcgtctcga
     8281 gattgtgaag gactgccgac aatgatttca ccgagggaaa ttccacgatc aaaagaatca
     8341 gcgccagcgc cgacaataga tactgatcgg ccaaaaagcg gcccaccaat gccaggatca
     8401 aaccgtagac tgcaatcgct tcaatgatgg caaagagcac gaccttgcgg gtgacgtaag
     8461 tcgacaccac cgccgcggcg cgttcttcct cgccgccttt gtatccttcc aaagcacgca
     8521 gccgtttgct cgtcttggcc tcacgcaata cgacctcggg cgccagacga cgtttccaat
     8581 agatgtagta gccgatgtca atgaaggcga gaagccaaaa caccgctctg gcggcgatag
     8641 cataagaaaa tccgcgcccg aaagcgggat ttgccaaaag gtgacgtgag atgaaaacgt
     8701 acacggcgat tgatatgaca aaaaaacacc agatgatgac ggccgcgccg cgctggtcca
     8761 gtaatattgt tttgaactcg ggacgaatca ttctgctaat atcctcgttc gtgtcgattt
     8821 agtccttcct tctaacttgc ggcgaaggtc tcgacgcgac taatagaata cgccattagt
     8881 tacattattc ttccgcgcat ctcctgtcca cggaaaacgt agagccatgc agtggccaag
     8941 tccaaacagc gtgcgcgcgt cttgtcggat aaaagctgcg atgctatagc ggcaaaagcg
     9001 attctacact ggaggcaaaa tggctgaatt cgatattgtc attcatggcg ggcaggtaat
     9061 cgacggaacg ggcaatcctt ggttctacgc cgatgtcgga attagagacg gcaagatcgc
     9121 cggcatcggt aagatcgatc ccgaagccgg ccaacgcgcg atttcggcga aaggctttgt
     9181 tgtgagtccc ggctatatcg acatgcacac ccacagcgat cagcccgtca tcgccgacgg
     9241 caatgccgaa agtaaagtgc gacagggggt gaccctggac atcatcggcg agagccagac
     9301 ggtggcaccg ctggaaggtg cagtgttgga agaatatcgg ctcgagcacc gaaagcgcaa
     9361 tggcatcgag gccgactgga aaacttttac cgattacttc agcaggctga tgaaggaggg
     9421 gacttcgatc aatgtcgcct ccggcgtgtc gccgcagcaa gtgaagcggg tcgtctgtgg
     9481 ctttgaagaa cggcctgcca gcgccagcga gcaagagaag atgaatcgac tggttgccca
     9541 agccatggag gagggcgctc tcgggctcac tgctgcatgg catgcaaaag gccccgagag
     9601 tccggaagaa gtggtcgaga tggccaaagt ggtcaagcgc tacggcggct attacggcgt
     9661 tcatgtcggc agcgaaggtt tcgacatgat ggaagagctg gcaaaaactc tgcgaattgc
     9721 ccgcgaatcc agaattccca tccatgttta tcacttgaag atgcgcgcaa aggccaattg
     9781 ggggcgcatt cgccaggtga tcgaacaaat cgaggaggcg cgccgcgaag ggctggaaat
     9841 taccgccaac caatatccct acacggctat gcagcatccc tggcgcaggc tatttccccg
     9901 ctgggttcag gatgcgccga tcaatgaaac gatttcacaa tttagaagcg catcgtttcg
     9961 cgagcgagtc atgaacgatc cggagtttga tcagtacgtc aacgaacacg gcggctggga
    10021 agggatcgtc gccgcccgct tggataccga gcagctgaag cgtttcgaag gtaaacctat
    10081 cgccgaaatc gctaaaatcc gcggtcagga tccggtgagc acctgcttcg acatgatcta
    10141 cgaagagggc gtgttcatcc atggcgtgca ccacaccatg tcggaagagg acgtcaagac
    10201 ggtgatgcgg gtgccctggg tgtcgatcgg ttccgacggc agcgctttga atcttaactt
    10261 cccgggcaaa ccccatccgc gcagcttcgg caccaacccg agagtgctag gcaagtacac
    10321 gcgcgaggag aaagtgctcg ccctcgagga tgccgtgcgc aagatgactt ctctgccggc
    10381 gcaagttctc ggcttgaaag atcgaggttt gttgcgcgaa ggctattggg ccgacgtggt
    10441 cgtgttcgat cccgatcggg tcgccgacat ggccacctat gagaatccca agcagtaccc
    10501 gaagggcatc gattacgttt tggtcaatgg cgcaatcgtc attgacaatg gccatcacac
    10561 cggcgcgcgg ccgggcaggg ttgtttatgg gccgggaaaa atggacgtat gaaatacagg
    10621 tcctgttttc agaagttatc agaagttaaa ggtggatcga atttgccaac aggttgggcg
    10681 tcgtgaatga ggagccgagc atggcatctt tgctgatctc ggacacatgg gaacgagggg
    10741 atccctatga gcagtatgtc ggccgctgga gccgcagggt cgcgccgcag ttcctctcgt
    10801 ggttgaacat tcctgcccgg cgtcgctggc tggatgttgg atgcggcacg ggagcgttaa
    10861 gcgccgcgat tctcgaccag tgtgcgccaa catcgatcgt tgccgtcgag ccttcggaag
    10921 gttttttgga gaaggccaaa gagcaactag gaagtagggt gctgcttcgg cgcggcagtg
    10981 ctgcggagat tccgctggat gacagagccg ttgatgttac tatttcggga ttggtcttga
    11041 acttcgttcc ggatggtcgc gccgctgtga gcgagatggc aagggtgaca gcaccgggag
    11101 gcacgatcgc ggcctacgtt tgggattact cgggaaagat ggagttgatg cgctacttct
    11161 gggatgcagc tgtcgagctt aagcccgagg cagcgaagct ggacgaaggg gtccgttttc
    11221 cactgtgcca tccggaggcg ctctcagaac tttttgccag cgcaggtctg cgtcgacccc
    11281 aggtgacggc aatcgacatc accacgagct ttgcaggttt cgaagattac tggcggccgt
    11341 ttctcggcgg ccagggaccg gctccggcgt atgctatggc gctcgatgac ccggaacggg
    11401 cgcgtctagc cgagcgcata cgccagcgcc taccgattca ggcgaacggt tccatctcct
    11461 taatcgctcg ggcctgggcc gtgcgcgcgg ccgcggcgag ctaagaatgt
//
LOCUS       DASUVS010000101         6896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1344970, whole genome shotgun sequence.
ACCESSION   DASUVS010000101 DASUVS010000000
VERSION     DASUVS010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344970"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VF182_05600"
     CDS             <1..802
                     /locus_tag="VF182_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6436585.1"
                     /translation="LKGAIMKLAGQVAVVVGGARGIGEAIAHLFSKEGASVVLVDIGK
                     AKSQLDGVAQAINQRGGNAIALVADATDDAQVNNVVNEVIERHGKIDVLVNSAGLRGP
                     LVPVQDITEEEWDLVLSVNLKAVFLCCRAVLKVMMKQKSGSIVSISGTAGKEGMALRG
                     SLCAAKWGLLGLTQTIAKEAGPCGIRANIICPGGMDEPDLRVMYAERAKGLGMEFSQL
                     EKSILELTPLRKYAKHEEVAKAALFLASSDSSHTTGESLNVSGGRLMN"
     gene            complement(928..1623)
                     /gene="sfsA"
                     /locus_tag="VF182_05605"
     CDS             complement(928..1623)
                     /gene="sfsA"
                     /locus_tag="VF182_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEX6436586.1"
                     /translation="MRFPDRLIRGVLIRRYKRFLADVRLANGRVVIAHCTNTGSMTGC
                     AEPGSTVYISPSTKEGRRLLYTWEIVKVGTTWVGINTLHPNRLVAESIEKGVISELQG
                     YTTIHREVITRQGTRLDLRLDGNNGFCFVEVKNVTRAIDGAAAFPDAVTERGTKHLKE
                     LIWLRRKGHRAAVVFVIQRGDCNYFRPADEVDSEYGYWLRRAVKAGVEALPYRARVTA
                     REVILTERLEARF"
     gene            1805..4021
                     /locus_tag="VF182_05610"
     CDS             1805..4021
                     /locus_tag="VF182_05610"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6436587.1"
                     /translation="MVKQSLKFVGQSVPRVDGIEKVTGQAKFLGDLAVPGMLHGKIIR
                     SSYPHARIISIDASKAEALEGVAAVLTSADLSDLNAIYSGRPVIAMDKVRYVGEPVAA
                     VAAVDQATAEEAAALIQVQYEELPVAVGIDMARAPGAALIHEGRSGNICSHESVVRGH
                     VQEAFAQSDLVLEDQFTFPMVYHYAMEPHTVTAHWDEDGVTVWSSAQHPFQVRGDIAK
                     IFRLPASRVRMVISYLGGGYGSKSYTKFEPLVVALARKAKAPVRVCNSVGESMITVRR
                     HAAQVRVKTGFKNDGTITAREAEIYLDTGAYDDNGPAVTVRAATRVIGPYRIPNILTH
                     AYSVYSNSGSAGSFRAIGAPQVIFAGESQIDIAARRLGLDPVELRQKNLLKRGEELRP
                     GLKGIDADLNYGLKKLVGASQWKSITKKKAAPVGLACAVTNAGATPVSVAMARLQSDG
                     LVRLFAGSTEMGQGVRTVLSQIAAEELSIPLDRIRVGGADTKVTPYDSSTGSSRSTTC
                     MGNAVQGAARDLKKQLAMIAAETFRVPQNQVKLQDGSLISGEARLTFQEALERRFGKG
                     SGGELIGHGETTPGLTENKMPVFWEVGMGMVELEIDEDTGEIGVKRLISVADVGKAIH
                     PEGCVGQEEGAAMMGIGHTLFEQMVYDANGNLLNPNLVDYRVPSFGDTPEEFRTVLVE
                     NEDGPGPYGVRGMGEGGLLSVAPSICNALERATGVRIKDLPLTPERVWRALKEARK"
     gene            4079..4579
                     /locus_tag="VF182_05615"
     CDS             4079..4579
                     /locus_tag="VF182_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6436588.1"
                     /translation="MSFWAKLPDFEIAPASIEDVPLILAFIKKLAEYERLAHDVVATE
                     DSLRRTLFEPRKTAEVAIGYFQKKPIGLVLFFHNYSTFLGRPGLYIEDLVVDEPYRRR
                     GFGSALLRYVARLAVERNCGRLEWSVLDWNQPAVDFYTKLGAVPMSDWTVFRVTGQNL
                     AKLAGE"
     gene            complement(4838..5494)
                     /locus_tag="VF182_05620"
     CDS             complement(4838..5494)
                     /locus_tag="VF182_05620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX6436589.1"
                     /translation="MRNTRSERKAVADALARERQRISRELHDRALQILSSVRLRTEVC
                     RDQFVAKPESLRRELEIIEKDIDTVILEMRNILTETQERKELKAGSLEQRLKAELEIF
                     RARTGFSLDFRCTIHARDLPEEIERELYFTLREGVLNAVRHARASQLTLALSRDASSC
                     NARLIDNGVGFDISTAEGGNHYGLRGMRERMKRIGGELTVDSAPGAGTRLLIRIPLDG
                     "
     gene            complement(5537..6250)
                     /locus_tag="VF182_05625"
     CDS             complement(5537..6250)
                     /locus_tag="VF182_05625"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6436590.1"
                     /translation="MAPSSWQQTTSDNSTMRMHLSVADGPGPFPGIVVMQHQWGIDEF
                     MQSITARLAQAGYVAAAPDLYHRDGAECTDDIIARRSRLSDRKVINDVNATVRFLRSQ
                     PAVDENRFAIIGFCMGGRIAYLMAAASFELKAAVVFYGGNIFRAWGRDIPSPFERTPE
                     IHCALQGHFGAVDRNPSPDDMRKLDFELTKYDKKHEFYAYPDAAHAFMDHTRESYRRH
                     ADEAAWPRTLEFLTRHLST"
     gene            complement(6319..>6896)
                     /locus_tag="VF182_05630"
     CDS             complement(6319..>6896)
                     /locus_tag="VF182_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX6436591.1"
                     /translation="RFRACFGASPPLTFGPVSRDSIEQLERDWWKKLVHCVFEPLGPF
                     EQFDRFFSELFSYFARAEAWALYPDVLKTLSALQERRLALDVISNFDSRLVDILEGLG
                     MNGCFEEVFISSRVGHAKPARQIFETALRRHNLAPSEAMHVGDSEENDLYGAANAGMM
                     GVLIDRSAKRPSTSYRQIQSLNEIIPILDQV"
BASE COUNT         1509 a         1813 c         1999 g         1575 t
ORIGIN      
        1 tctgaaagga gcaatcatga aattagctgg ccaggtagcc gtagtggtgg gtggcgctcg
       61 aggtatcggc gaggcaattg cccacctctt ctctaaagaa ggcgccagcg ttgtcttggt
      121 cgatatcggt aaggccaagt cgcaattgga tggcgtggct caagcaatta accaacgggg
      181 cggcaatgca atcgctcttg ttgccgatgc cactgatgat gctcaggtta acaacgtggt
      241 gaatgaagtt atagagcgcc atggcaaaat cgatgtcctc gtcaacagcg cgggattacg
      301 cgggccgctc gtcccggttc aagacatcac tgaggaggaa tgggatcttg ttctttcagt
      361 taatctgaag gctgtgtttc tgtgctgcag ggcagtgtta aaagtgatga tgaaacagaa
      421 aagcggcagc atcgtcagta tctccggaac ggctggcaag gaaggtatgg ccctgcgcgg
      481 ttctctttgc gccgccaagt ggggattatt ggggttgact cagaccatcg ccaaggaggc
      541 cggaccctgc ggcatcagag ccaatatcat ttgtcccggt ggaatggatg agcccgacct
      601 gcgggtcatg tatgcggaac gggccaaggg acttggaatg gaattctcgc agttggagaa
      661 aagcatcttg gagctgacgc ctttgcgaaa atatgccaaa cacgaggaag tcgcaaaagc
      721 cgctttgttt ctcgcatcga gcgactcatc gcacaccacg ggagaatcgc tcaacgtttc
      781 cggcggacgg ttgatgaatt gaggtttcga gcgattccct gctgtggaca tgccggttag
      841 acagagctaa gcgcgcgatt catcattatt ggctaagttg attgtcaccc cgagggctgg
      901 agctgcgtcc gtacagttgg agactgatca gaatctcgct tcaagccgct cggtcaggat
      961 cacttcccga gccgtgactc gtgctcgata aggcagcgct tccacgcccg ccttaacggc
     1021 tcggcgcaac cagtaaccgt actccgagtc gacttcatcg gccggtcgaa aatagttgca
     1081 gtcgccgcgt tggattacaa aaactaccgc ggcccgatgg ccttttctgc ggagccagat
     1141 caattctttc aaatgtttag tgccgcgctc ggtaactgca tcgggaaatg ccgccgcacc
     1201 atcaatggcg cgtgtgacat ttttaacttc gacgaaacaa aagccattat tgccatcgag
     1261 ccgcagatct agtcgtgtgc cctggcgcgt aatcacctcg cgatgaattg ttgtatatcc
     1321 ctgcagttcg ctgatgacgc ctttttctat ggattcggcg acgagccggt ttggatgcag
     1381 ggtgttaatg ccgacccaag ttgttccgac tttgacgatt tcccaagtat acagcaaacg
     1441 gcggccttcc ttcgtgctgg gtgaaatata caccgtgcta ccgggttcgg cgcagcctgt
     1501 catgctgccc gtattggtgc agtgggcgat gacgactcgg ccgttagcca gccggacatc
     1561 ggcgagaaaa cgtttgtatc ggcgaataag aacgccgcgg atcagccgat caggaaaacg
     1621 catgaacaaa gttatagccg atgtgtcaac tgattccgac cattcgctgg ttgccgatct
     1681 cggagccgta tcatcgatcg attctcttgc cgtcgatttt tacacatgat acgccgcggc
     1741 tttccttgcg cgggaaacag ccctttgtta ggttcaacga aaattagtgg gtggatggag
     1801 agcaatggtc aaacaatcgc tcaagtttgt cggtcagagt gttccgcgcg tcgatgggat
     1861 tgaaaaagtc acaggccagg cgaaatttct tggcgatttg gctgtgccgg ggatgctcca
     1921 cggaaaaatt attcgcagtt cctatcctca cgcgaggatt atctcaattg atgcgtccaa
     1981 agccgaagcg cttgaaggcg tcgccgcggt actgaccagt gctgacctat cggatctcaa
     2041 cgcgatctat tccggccgtc cggtgattgc gatggataag gttcgttacg ttggagaacc
     2101 cgtagcagct gttgcggcag tagatcaggc cacggccgaa gaggctgccg cgctaatcca
     2161 ggttcaatat gaagagcttc cggtcgctgt cggtatcgac atggcgcgcg ccccaggcgc
     2221 agccctgatc cacgaaggac gaagcggtaa catctgcagt cacgagagcg tggtccgcgg
     2281 ccatgtgcaa gaggcgttcg cccagtctga tttggtcctc gaagaccagt tcacgtttcc
     2341 gatggtttat cattatgcga tggagcccca cactgtcacc gcccattggg atgaagatgg
     2401 cgttactgtg tggtcttcgg cgcagcatcc cttccaggta cggggcgata tcgccaaaat
     2461 atttcgtttg ccggcgtcga gagtgcggat ggtgatctca tatttgggtg gaggctatgg
     2521 cagcaagtcc tacaccaagt tcgagccgct ggttgtcgcc ttggcgcgca aagcgaaggc
     2581 gccggttcga gtgtgtaact cggtcggcga gtccatgatc acagtccgcc gccacgcggc
     2641 gcaggtgagg gtcaaaaccg gttttaagaa cgatggcacg atcaccgccc gggaagcgga
     2701 aatctacctc gacaccggcg cctatgatga caacggcccg gccgtgacgg ttcgtgcggc
     2761 aacacgcgtc atcggtcctt atcggattcc taatattctg acgcacgcgt attcggtcta
     2821 tagcaactcg ggttccgcgg gttcgtttcg cgcgatcggc gcgccgcaag ttattttcgc
     2881 aggcgaatcg cagatcgaca ttgccgcgcg ccgtttaggc ctggacccgg tggaattgcg
     2941 ccagaaaaat ctcttgaaaa gaggagaaga acttcgtcct gggcttaaag gcatcgatgc
     3001 cgatttaaat tacggcctta agaagctcgt cggcgcaagc caatggaaat ccataaccaa
     3061 gaagaaggcg gcgccggtcg gattagcctg cgccgtgacc aacgccggag cgaccccggt
     3121 ctcggtggca atggcacgcc tgcagtctga tggcctcgtg aggcttttcg ccggcagcac
     3181 cgagatgggg cagggagttc ggacggttct ttcccagatt gccgccgagg aattgagtat
     3241 tcccttggat cgtatccgag tcgggggcgc cgacaccaaa gtcacccctt acgatagctc
     3301 gacgggttcc agccgatcca ccacctgcat gggcaacgcg gtgcaaggcg ccgcccgaga
     3361 tttgaaaaaa caattagcta tgattgctgc ggaaacattt cgcgtgcctc agaaccaggt
     3421 caaactccaa gacggctccc tgattagcgg tgaggcgcgg ctgaccttcc aagaagcgct
     3481 cgagcgccgg ttcggcaaag gctcgggcgg cgagttgatc ggacacggcg agacgactcc
     3541 gggattgacg gaaaacaaaa tgcctgtgtt ctgggaagtg ggcatgggca tggtcgagct
     3601 cgagatcgat gaagataccg gtgaaattgg cgtcaagcgt ttgatatccg ttgctgacgt
     3661 cggcaaggcg atccatccag agggctgcgt tggccaggaa gagggcgcgg ctatgatggg
     3721 catcggccat acgttgtttg aacagatggt ttatgacgcc aacggtaatc tgctcaaccc
     3781 gaatctggtc gattaccggg tgccgagttt cggcgatacc cctgaggagt tccgtacggt
     3841 cttggtcgag aacgaggatg gccccggtcc ctatggggtt cgtggtatgg gtgaaggcgg
     3901 cttgttatcg gtcgcgcctt cgatttgtaa tgcgctggag cgggccacag gagttcgtat
     3961 aaaagacctg ccgttgactc ctgagcgtgt ctggcgcgcc cttaaagaag ctaggaagtg
     4021 agttctttac cggctcccac gacgaatggg acgataatgg accgtggcaa gaggacgcat
     4081 gagtttctgg gcgaaattgc cagactttga aatagcgccg gcatcaatcg aggacgtgcc
     4141 cttgattctt gcgtttatca agaagcttgc cgagtatgaa cggcttgcgc atgacgtcgt
     4201 cgccaccgaa gattcgttga ggcggacgct gtttgagccg cggaaaacag cggaggttgc
     4261 gatcggctat ttccagaaga aaccgatcgg gttggtgctg ttcttccaca actattcgac
     4321 ttttttgggc cggccgggac tctacataga ggatctcgtc gtcgatgaac cataccgtag
     4381 gagggggttt ggctcagcgt tgttgcgcta tgtggcgcgc ttagccgtcg agcgaaactg
     4441 tggccggctc gaatggtctg tgctcgattg gaatcaaccc gccgtagatt tttataccaa
     4501 gctcggcgcc gtcccaatga gcgattggac cgtgtttagg gtcacggggc agaatttggc
     4561 gaagctggcc ggggagtgaa ccgtcgggcg ccgcattggc agcttcgcga caaagacctg
     4621 atgagaatca attggcgctt tttcgattgt attccgctgc ggatgttcgc ggatgttgct
     4681 accagtcaga ccgagtttct acggcagatg tggcgctgat tgtcccttca atctgcgcga
     4741 gtgtcggcct ggtttgttgg cgagccgcgt gaaccatgca taaatacgat gtcgctcgtg
     4801 agctaccggt ttcttgccga aatcccggag cattcactta tccgtccaga ggtattctga
     4861 tcaggagccg tgtgccggct cccggcgccg agtctaccgt gagctcgccg ccgatcctct
     4921 tcattcgctc acgcatgcct cttaggccgt aatgatttcc gccttccgct gtcgaaatat
     4981 cgaagccgac accattatcg atcaaccggg cgttgcagct cgaggcatct ctggagagcg
     5041 ccaatgtcag ttgtgatgcg cgggcatgtc gcacggcatt tagcacgcct tccctcagag
     5101 tgaagtacaa ttctcgttcg atctcctcgg gcaagtcacg cgcgtggatg gtgcagcgaa
     5161 aatccaagct gaaaccggtg cgcgcgcgaa agatctccag ttctgccttg agtctttgct
     5221 ccaacgagcc cgccttgagt tcttttcgct cttgggtctc ggtgagaatg ttgcgcattt
     5281 cgaggatgac tgtgtcgatg tctttctcga tgatctctag ttcccgtcgt agagattctg
     5341 gcttcgccac gaactggtcg cgacatactt cagttcttaa tctgacgctt gataaaattt
     5401 gcagcgcgcg gtcatgaagc tcgcgcgaga ttcgttggcg ctcgcgagct agggcgtcgg
     5461 ccacggcttt gcgttccgat ctcgtattgc gcacgttgtt gtccttgatc gacaatgcgt
     5521 ctaggttaca gaacggctaa gttgacaaat gcctggtcaa aaactccaaa gttcgtggcc
     5581 aagcagcctc atccgcatga cggcgatagc tctcacgggt atggtccata aaggcatggg
     5641 cggcgtctgg gtatgcatag aattcgtgct tcttgtcgta cttcgtaagc tcaaaatcga
     5701 gcttgcgcat atcgtcgggc gacgggttcc tatcaacggc gccgaaatga ccctggagcg
     5761 cacaatgtat ttccggagta cgctcaaatg gcgaaggaat atcgcgaccc cacgcgcgaa
     5821 agatgttgcc gccgtaaaaa acgacggccg ctttaagctc gaaacttgca gcagccatga
     5881 gataggcgat acgacccccc atgcaaaaac cgataatcgc aaaacgattc tcatcaaccg
     5941 ccggttgaga tcgcaggaat ctcacggtcg cattgacatc attgataacc ttccgatcac
     6001 tgagccggga acggcgtgca atgatatcgt cggtgcattc ggcgccatcg cggtgataga
     6061 gatccggcgc agcggcgacg tagccggcct gcgctagtcg cgccgtgata ctctgcatga
     6121 attcgtcgat gccccattgg tgttgcataa ccacgatgcc cgggaatgga ccgggtccgt
     6181 cggcgacact caagtgcatg cgcatcgtgc tgttgtcgga cgtagtttgt tgccagctgg
     6241 atggtgccat tttcattcct tcaaagttag caggcgttta ccgtagcggc cctaccgtgt
     6301 caagtcttga gccagccttt aaacctgatc gagaattgga ataatctcgt tcagactttg
     6361 aatctggcgg tagcttgtcg agggtcgctt cgcgcttcga tcgatcagaa ctcccatcat
     6421 tccagcattg gcggcgccgt ataaatcgtt ttcttcgcta tcgcctacgt gcatggcctc
     6481 gctgggagcc aggttgtgcc gtcttaaggc cgtttcaaag atctgccgcg caggtttggc
     6541 atgaccgacc cggcttgaaa taaaaacctc ttcaaaacaa ccgttcatcc cgagtccctc
     6601 gagtatatcg accagccgag aatcaaaatt agaaataaca tctaacgcca agcggcgttc
     6661 ttgcaacgcc gacaacgttt ttaggacatc agggtataac gcccaagcct ctgctcgcgc
     6721 aaaatatgaa aacagctcgg aaaaaaaacg atcgaattgc tcgaacggtc ccaagggctc
     6781 aaagacgcaa tgcactagtt ttttccacca atcgcgctcg agctgctcga tcgagtcccg
     6841 cgacaccgga ccaaacgtga gcggcggtga ggcgccaaaa caggctcgaa agcgtt
//
LOCUS       DASUVS010000102        35114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1563499, whole genome shotgun sequence.
ACCESSION   DASUVS010000102 DASUVS010000000
VERSION     DASUVS010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 35114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35114
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563499"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="VF182_05635"
     CDS             complement(<1..1287)
                     /locus_tag="VF182_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX6436592.1"
                     /translation="MDLFESDQSKQQSARAPLAERMRPRNLDEFVGQKHLLGPGRILT
                     EMVESAQLRSLVLWGPPGSGKTTLAQILASKSGALYVQFSAVSSGVKDLKKIIDEAER
                     LRRMGKKTVLFVDEIHHFNKSQQDNFLPYVERGTIVLIGATTENPSFEVISPLLSRTQ
                     VLVLKALSTEEIAAIVDRALQDKERGLGNMHLNLSPEGREFLIDQCQGDCRVALNALE
                     TAATLAHNAKAKEIGLTQLQEALQRKPLRYDKSGEEHYNIISAFIKSLRGSDPDAALY
                     WMMRMIEAGEDPLFIARRMVIFAAEDIGNADPAALQVAVAAKDAFHFVGLPEGRIPLA
                     QAVTYLATAPKSNASYLAMLAAAQDVEQCGALPVPLHLRNAPTHLMKKLGYGKDYQYA
                     HNFPDHVVDQQHLPDELKTQKYYEPSESGYEKQIKER"
     gene            1485..1856
                     /locus_tag="VF182_05640"
     CDS             1485..1856
                     /locus_tag="VF182_05640"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX6436593.1"
                     /translation="MAEKSESNGSSPPLVQGTVRTVLFPDSAISGRLSYDLPVKIDSK
                     FTGEVKASDLLVVGANAHVEARIAARNLQLEGKLIGSVQVIGCFEIMPGGHFQGDVQA
                     AELKVHPGATFDGQGKITGEP"
     gene            complement(1865..2221)
                     /gene="rplS"
                     /locus_tag="VF182_05645"
     CDS             complement(1865..2221)
                     /gene="rplS"
                     /locus_tag="VF182_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012360058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX6436594.1"
                     /translation="MNILDKLETLQSKQEIPALRAGETVRVHVKVVEGEKERTQVFEG
                     IVIGIGGKGNRANFVVRKISYGVGVERIFPFHSPRIDKVEVVSRGRVRRAKLYYLRDR
                     SGKAARLFSDERSGNR"
     gene            complement(2237..3511)
                     /gene="trmD"
                     /locus_tag="VF182_05650"
     CDS             complement(2237..3511)
                     /gene="trmD"
                     /locus_tag="VF182_05650"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX6436595.1"
                     /translation="MVTFTIVTLFPAMFASPLNHSILKKAQEKGLISIVLVDPREYTT
                     DRHRMTDDYPYGGGQGMVMKPEPLVTAIEDVKAKMPRSKVILLSPQGRVFNQAVAVEL
                     AKEEELVLICGRYEGVDERVKGFVDEEISIGDYTISGGEPAATVVIDALSRLIPGVLG
                     NAKSAADESFSNRLLEYPQYTRPEEFHGAKVPDVLLSGDHERIRQWRRHMSIQQTWKR
                     RPDLIRSAELSAEEKELICNQRAPVYVALVHYPVYDKNRQIVTTAVTNMDIHDIARSG
                     RTYGIEGFYVVTPVKALQRLALKIIDHWEHGYGSQYNTTRKEALALVRVKNTLDDVLI
                     DLEQEYGEKPRIVVTSARPGGQRTSFAELQDMLINTTHPFLIVLGTGWGLTETIISQS
                     DYLLDAVEGWTDYNHLSVRSAAAIILDRLLGR"
     gene            complement(3533..4045)
                     /gene="rimM"
                     /locus_tag="VF182_05655"
     CDS             complement(3533..4045)
                     /gene="rimM"
                     /locus_tag="VF182_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX6436596.1"
                     /translation="MPNGIVPLGQIVTTHGIAGWLKLNPYNSESPILSTLNEVILVQD
                     ETRTVVQVESARPHKRQILLKLCGTDDIESASRWVGSTVSVPEEALEIPAPGQYYHYQ
                     VIGLEVFDINGTRIGTLKEIWSTPASELYVIAGADKEHLIPAVKEIIAEVDLATGRVI
                     VDLPAGLLDL"
     gene            complement(4067..4303)
                     /locus_tag="VF182_05660"
     CDS             complement(4067..4303)
                     /locus_tag="VF182_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX6436597.1"
                     /translation="MKELVQYLAKSLVNNPDAVEVAESAAEDAAVYELRVAKDDLGRI
                     IGKQGRTAKSIRAILNAAASKTNRKVVLEIVEEK"
     gene            complement(4326..4571)
                     /gene="rpsP"
                     /locus_tag="VF182_05665"
     CDS             complement(4326..4571)
                     /gene="rpsP"
                     /locus_tag="VF182_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX6436598.1"
                     /translation="MSVRIRLSRHGAKKKPFYRIVVSDQRFARDGRYIEQIGTYDPSA
                     GGGTFKINQEKAETWIKRGAQPTQTVSQLIAKQKKAS"
     gene            complement(4585..5925)
                     /gene="ffh"
                     /locus_tag="VF182_05670"
     CDS             complement(4585..5925)
                     /gene="ffh"
                     /locus_tag="VF182_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX6436599.1"
                     /translation="MFESLTEKLELTFKRLRGEGKISEKNIDDALRELRLALLEADVH
                     VKVVKTFLDSVKAKSLGQEVLLSLTPEQQFLKIVRDELTGLLGGDHRKLDLKASPPVV
                     IMLVGLQGSGKTTTLAKLARHLKKHEKRTPYLVPADVYRPAAIEQLKILGAEIEMPVY
                     DSNPQTSPVAICKQALDEARKRFCDLLLIDTAGRLHIDEELMQELQQIKGTVNPHQIL
                     FVADSMTGQDAVNQALGFDQQLGVSGVILTKLDGDARGGAALSIRQLVGKPILFAGTG
                     EKLDALEPFYPDRLASRILGMGDVLSLIDKVQQNFEQKEAERLQKVIQKQQFTLEEFQ
                     IQLQQIKKMGSMGSLLELIPGGKKLASQVDADKAERELKRVEAIINSMTLQERRSPAI
                     LNGSRRRRIAQGSGTSVADVNRLMKQFLEMKKMMQRVSKGGMKSLFSRMANPFH"
     gene            complement(6076..7731)
                     /gene="groL"
                     /locus_tag="VF182_05675"
     CDS             complement(6076..7731)
                     /gene="groL"
                     /locus_tag="VF182_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528688.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX6436600.1"
                     /translation="MASKIVKFNRDARDAMLRGVNILADAVTVTLGPKGRNVVLDKSF
                     GAPNVTKDGVTVAKEIELEDKFENMGAQMVKEVASKTSDVAGDGTTTATVLARQIFAE
                     GVKLVAAGHDPMTLKRGIDKAVEAVTAELKGLSKPTKDPKEIAQVGTIAANNDSTIGD
                     VIAEAMNKVGKEGVITVEEAKGLETTLDVVEGMQFDRGYLSPYFVTDPERMECILEDA
                     YLLIHEKKISNMKELLPVLEQIAKSGKPFIIIAEDVEGEALATLVVNKIRGTLHCVAV
                     KAPGFGDRRKAMLEDIAILTGGKLIAEELGVKLENIALQDLGRAKRIVVDKDNTTIVD
                     GAGKKADLEGRIKQIRAQIEETTSDYDREKLQERLAKLIGGVAVINVGAATEVEMKEK
                     KARVEDALHATRAAIEEGIVPGGGVAILRAAAGLDKISVDEDERWGVNIIKRVSEEPI
                     RRIAMNAGVEGSIVLQKVKENKGAFGFNAATEQYEDLMKAGIIDATKVVRTALQNAAS
                     VASMLLTTEAMVAEKPEEKSAMPPMPPGGMGGMGGMGGMGGMM"
     gene            complement(7762..8052)
                     /gene="groES"
                     /locus_tag="VF182_05680"
     CDS             complement(7762..8052)
                     /gene="groES"
                     /locus_tag="VF182_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX6436601.1"
                     /translation="MKIRPLQDRIIVKRIEEEEKSKGGIIIPDTAKEKPQEGKVVAVG
                     KGKVSEDGKITPLDVKVNDRVLFGKYSGSEINIDGEEHLIMREDDVLGVLEK"
     gene            complement(8219..8497)
                     /locus_tag="VF182_05685"
     CDS             complement(8219..8497)
                     /locus_tag="VF182_05685"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HEX6436602.1"
                     /translation="MSRLSASRISHLAHLVVSGLNRDDVAEFPNEGRALSETKRILHE
                     FFEREDRFDDLVRQKIQSLSRHIPPGSREWDVLYRKYLEEEMRRQKLK"
     gene            complement(8494..8769)
                     /locus_tag="VF182_05690"
     CDS             complement(8494..8769)
                     /locus_tag="VF182_05690"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HEX6436603.1"
                     /translation="MKIKPEELDKLARLLLENYRAKELIRPKREDPIILAKIRGVISR
                     NFAEEEAIEDEARKMLAAHSQATREMDHFKMFLIAKQKLAAKKGFIL"
     gene            9069..9980
                     /locus_tag="VF182_05695"
     CDS             9069..9980
                     /locus_tag="VF182_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX6436604.1"
                     /translation="MIIKKPPRLRPGHRIGIIAPAGCVERDALSRGVQVIRDQGFEVE
                     LSPRIFDTKGYLAGDERNRAEDLVNFFQRNDIGAIFCARGGFGSAQLLPFLIHLNFSP
                     VPKIFAGYSDVTFLLNWIHSRFGIVTFHAPMVAMELARGLRGRTQEAFWGSVTGDKAE
                     WQVELGEVIRPGKAEGTMMGGCLSVLVTTLGTPYEIQTHGKLLFLEDVGERPYRIERM
                     LTHLKMAGKLDHLAGLVFGDFSNCEAEGSRSVPRIINEMFAEAPYPVLTGLPAGHGSE
                     HMTLPFGVKMILDGNTRSLSLIESPVA"
     gene            10024..11148
                     /locus_tag="VF182_05700"
     CDS             10024..11148
                     /locus_tag="VF182_05700"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX6436605.1"
                     /translation="MTMDFAAIEDATEEAVKQGVFPGAVLLVGGGEDVIYERAFGWRS
                     LLPDRTPMRSSTIFDLASLTKPLATALGIMLLVNENKLQLDDQVTQFVPSLANTPTKI
                     RHLLNHTSGLPAWKPYYEEVIHCVDSGRTGFIGSAGAKSYILGQLHREALLSEPGSQA
                     LYSDLGFMLLGEIIENISGTNLDVFFHHRVFQPLKLRSATFFNVAISPTSRIQNEEDE
                     IAPTELCPWRKKVLCGEVHDDNAFAAGGVAGHAGLFSCARDIHAIVAKLNRCLQGCDS
                     FLSQELVQEFFTKDTSVPGSTHALGWDTPAPEKSSSGMHFSRKSVGHLGFTGTSLWWD
                     LEKNCHVILLTNRVHPNRNNERIKQFRPKIHDLIMRAINL"
     gene            11145..12566
                     /gene="mpl"
                     /locus_tag="VF182_05705"
     CDS             11145..12566
                     /gene="mpl"
                     /locus_tag="VF182_05705"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEX6436606.1"
                     /translation="MNQLPAKGSHIHLIAVCGVGMASLAGLLKAHGYRVTGSDQNVYP
                     PMSAYLAQVGIDVQNGYDAQHLADRPDLAIIGNAVSRGNPEAEAVLDSTIPYLSFPQA
                     LGKFLIGTRQSIVVTGTHGKTTTTALTAWVLKCAGLDPGFFVGGVPLNFASGWHPGES
                     DQVILEGDEYDSAFFDKGPKFLHYRPQSVILTSVEFDHADIYRDLNHLKEAFRSLARL
                     VPATGLLVACYDYPAVREVVKAGSCRVVSYGDDGICDWTVRDVRSEGRRSYFDPCFRN
                     ASEGTVELGAIGRHNVKNALGVYVLARELGMERNNLLEGFASFSGVKRRQEFKGEKRG
                     ILVIDDFAHHPTAVAETIKAVKDAFSGRRLWAVFEPRSNTSRRNVFEREFSVALSLAD
                     RVVVSGLHQPDKIPEAERLSVERVVDAINQSAGREQAVVINGADNIATHVSTRATAGD
                     IVLVMSNGAFDGVQDKILSGLAS"
     gene            complement(12824..13327)
                     /gene="folK"
                     /locus_tag="VF182_05710"
     CDS             complement(12824..13327)
                     /gene="folK"
                     /locus_tag="VF182_05710"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX6436607.1"
                     /translation="MAAAVAHQVYIGIGSNVGQKKDNFLEALDRVAKLPDTKILKESS
                     LYESEPLGDSRDWYVNGAIEIETKFKPEMLLNKFKNIERAMGRKKVKKRWGARIIDLD
                     ILLYDGLVMKKKNLHIPHPEMPNRKFVLIPLSEIAPQVIHPELGVTISELLVNVKDEK
                     KIHLFHP"
     gene            complement(13348..14520)
                     /locus_tag="VF182_05715"
     CDS             complement(13348..14520)
                     /locus_tag="VF182_05715"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX6436608.1"
                     /translation="MPILIKKAKRINDLPPYLFAEIDRRKREALARGVDLIDLGIGDP
                     DIPTPAAIVEKLIERASVPVNHRYPNSSGLPEFRQAVANWYQRRFGVKLNAGKEVVSL
                     IGSKEGIGNMAVAYVDPGDIVLVASPCYPVYHIGTAFSGGRNYFLPLKKENQFLPDLD
                     SIPSEIAKQAKLLWINYPNNPTAAVADRSFFERVIDFATKNNVLVCHDAAYSEMGYDG
                     YRPMSFLEFEGAREVGIEFHSLSKTFNMTGWRIGMAVGNAEIVSGLAQAKSNLDSGIF
                     QAIQEAGIEALQLGDSIIEPSRKIYQERRDILVSGLRAIGLECDKPRATFYVWVATPK
                     GLSSAEFTAKLLEEAGVVTTPGNGFGDAGEGYVRFTVCVDQQRLKEVTERIRQVNL"
     gene            14627..15112
                     /locus_tag="VF182_05720"
     CDS             14627..15112
                     /locus_tag="VF182_05720"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX6436609.1"
                     /translation="MDIIVDGYNLIGSERGLTGGLEHQRNWLLHRLSHYQELRGHTVV
                     LVFDGWKSGLTDEVSQDKQGVQVVFSRQGEKADSVIIRLARAKGSGCVVVTSDREVRR
                     AVEKFGAAAIYADEFTRILRNLDLADAGSQEGERRPNKKGNPKRLAKSERQRLGKLKK
                     L"
     gene            complement(15131..15781)
                     /locus_tag="VF182_05725"
     CDS             complement(15131..15781)
                     /locus_tag="VF182_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436610.1"
                     /translation="MRLVASMVLLIVFCLLTSAAASHHYTEKQLDALETRVGKKFWVA
                     AVEGKLPSFVTTPISDASVFQPSDNESFEITELVGRKARNPYYKVKFESGAEGYIQAQ
                     VFHEQFNRTILTVDPRADERKALAEKNQEEKARVEWIQSQPWSSAVKESAIKRQAVPG
                     MSAGEVKRVLGDPRRVSRIKGPQHANEEHWFYPDGTLLVFRNGAVNRIETTKKLEH"
     gene            complement(15926..16002)
                     /locus_tag="VF182_05730"
     tRNA            complement(15926..16002)
                     /locus_tag="VF182_05730"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15966..15968),aa:Met,seq:cat)
     gene            16064..16489
                     /locus_tag="VF182_05735"
     CDS             16064..16489
                     /locus_tag="VF182_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6436611.1"
                     /translation="MSEKPATPLTTVDTIIEISSGGIVLVERKNQPHGWALPGGFVDY
                     GESLEVAAVREAKEETCLDVTLVEQFHTYSDPHRDPRHHTVSTVYLAIADGVPEGGDD
                     AQDAKVFIENELPDRIVFDHPKILADYFRYKKTGQRPKP"
     gene            complement(16495..17697)
                     /locus_tag="VF182_05740"
     CDS             complement(16495..17697)
                     /locus_tag="VF182_05740"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX6436612.1"
                     /translation="MTTLAERTLLIKPSVTLAIAAKAGKLRSEGIDVINFSAGEPDFD
                     TPDRIKAAAIEALKKGMTKYTDVRGVEPLRQAIARKYLSEYGLSYQLSEILVSCGAKH
                     SLYNLFQAVVNPGDEVLVPAPYWVSYADMALLAGGVPKTIQTTEASGFRITAQQLAEA
                     LSSRTRVFVLNSPSNPTGASYEENELMAMAQVLEKHDCLIVTDDIYERIVYDRFTIQN
                     LVALAPSLRDRVIIVNGVSKTYAMTGWRIGYALGPADIIGAAAKIQSQSTSNPTSIAQ
                     AAALEALSGPQEDVAMMVGEFQKRRDVIVDRLDAIPGVECLKPQGAFYVFPNIAALIG
                     KTINGKKLASPCDIADYLLEEATVAAVPGEDFGSNAHIRLSYATSLENVEEGCRRMRQ
                     AFGKIQSS"
     gene            complement(17698..18195)
                     /gene="coaD"
                     /locus_tag="VF182_05745"
     CDS             complement(17698..18195)
                     /gene="coaD"
                     /locus_tag="VF182_05745"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX6436613.1"
                     /translation="MTQNIAIYPGSFDPITNGHVDLVKRALRVFDKVIVAIASNAGKD
                     TSLFSVEERLEMIREVFDALRDRVEADSFQKLLVDYAERKQAPVIIRGLRAVSDFEYE
                     FQMAMMNHRLKPNVETFFMMTGESQFYISSRLVKEVVGLGGNVEGLVPNNVLKKLNRK
                     FRQEQ"
     gene            complement(18199..18780)
                     /gene="rsmD"
                     /locus_tag="VF182_05750"
     CDS             complement(18199..18780)
                     /gene="rsmD"
                     /locus_tag="VF182_05750"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX6436614.1"
                     /translation="MRVIGGNARGRRLKVPKGQAVRPTSNRVKEALFNILPHDLSGIK
                     LLDLFAGTGNVSIEAISRGAAEAILIDSSDKSGKVIAENLRNVGFTDRANVWIAPVSR
                     ALRSLARQGESFDMIFLDPPYQHNFINACLEVIARGNLLRASGTLVVEHSARERPEAE
                     YGSLILKDQRKYGDTRLSFFKHKAAWSQPNEDT"
     gene            complement(19142..19495)
                     /locus_tag="VF182_05755"
     CDS             complement(19142..19495)
                     /locus_tag="VF182_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX6436615.1"
                     /translation="MTETIDKVAVDLKLTGKAVEQVKSLLARSEYIDHALRISVSDGG
                     CSGFSYQLNFEKEPKPGDIILDCETLTVYIDPESIPFLQGTVIDFVSGLYGGGFKFAN
                     PNATGTCGCGTSFSA"
     gene            complement(19579..20502)
                     /gene="ftsY"
                     /locus_tag="VF182_05760"
     CDS             complement(19579..20502)
                     /gene="ftsY"
                     /locus_tag="VF182_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX6436616.1"
                     /translation="MAGSFFQRLKDGLTKSRETWVQKMGSIFQNREWDEQSLEEMEES
                     LLTADVGVKATQKLMDTLRGLSPSGSEDLAQDMSSQLQKAMVELLQDSAAAPLVQPLS
                     IRPWIVLFLGVNGVGKTTTIGKLAAQYKADGKKVLLVAGDTFRAAAVEQLEAWGRRAG
                     VELIKHRTGSDPSAVVYDGMQAAKSRGIDVLLIDTAGRLHTKVHLVEELKKIRRVIAR
                     EQPGAPHETLLVLDATTGQNGLQQAKVFKEATDIDGIVLTKLDGTAKGGVIISIQEEL
                     GVPVRYIGVGEDIDDLQAFEAGRFVEALFER"
     gene            complement(20571..24122)
                     /gene="smc"
                     /locus_tag="VF182_05765"
     CDS             complement(20571..24122)
                     /gene="smc"
                     /locus_tag="VF182_05765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX6436617.1"
                     /translation="MRLKRLEMVGFKSFADKTLVELNPGITAIVGPNGCGKSNIVDAL
                     RWAMGEQSARHLRGHQMEDVVFAGSDSLPATGMAEVSLTFDNEDGRGPVEYSSFSEIM
                     VTRRLFRSGESEYSINKTPCRLKDVIELFLGSGVGSKAYSIVEQGRVDELVNSKPEER
                     RVLIEEAAGTSKYKSRKLVAERKLERTQQNLFRVTDIVREIERQIRTMELQAKKAERY
                     KILKAELREKDLLCANLHRQALNQEIATHENRLADAENRLAEYLNNLHGKESESEAVR
                     FSLMELDKEISLQQENVYQAKMQLQSEEQRIDLFNKDLSQLDENEAEGLSALAQLEDK
                     LRTLAREIEELNKAKESFIQLSLFEETFLRENETELERLQAEIHALEYGLETEKAALI
                     DIANQLVNLKNTLTDKERRRDETERALERSQREHSAAASSRELIEGKRNEAERALEAS
                     LEQSEERNLEFAQVVALIQGLTRSRDEQEERIEALKERIQESRSRLLSLEDLQKNYEG
                     YQEGVRAIMLKRQEEAAADGVYGLVTEVIEAPVLYEKALTAVLGDRLQYMIVKGHDEG
                     IEAIEFLKQKASGRGSFIPVQMSRKSNRPLPLGEAEVVAPLLELISVKEGYRDVAEYL
                     LSDVVVVQDLRSGLALWDRNGYYSTLVTPEGEVIDPMGVVTGGSEESLEGSFLTQRRR
                     MKELRGLLTELEADMQIEERELLELKKNLQQAEAKKTLLGSEIHRLELERVRNEHEHR
                     STQQDSQRLRQTIDVLLQEQRDLNATVANLMQEITEANAGREKRALEKAEREERLAGL
                     QTRFLERKQSLESVETAVTQSRIRAAALGEKRENTHANLDNRLKLQEEAAQQIGDRRG
                     QIADIHRRRGEIQNALTETEQTVASSRATVQELEEKLQNERQRYRQISKQLTEIDESV
                     KELRPRVETSQDEKNRLQVGLTEKRLNLQHLADGVREKYGLDLASMEAPNVIATASNE
                     LTIEIDELRARIERMGDVNMAAIGEYEELTTRFQFMNRQKEDLEKSIADLQQTIVKLN
                     RVCRVRFKESFEEINEKFQSIFPRMFRGGKARLVLTDENDYLETGVDIVVQPPGKKLQ
                     SITLLSGGEKALTAVSLLFAIFLTKPSPFCFLDEVDAPLDDANVDRFNELIKEMSEFS
                     QFVLITHNKKTMQAAEVLYGITMAEPGVSKVVSVRMS"
     gene            complement(24271..24903)
                     /locus_tag="VF182_05770"
     CDS             complement(24271..24903)
                     /locus_tag="VF182_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020478752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX6436618.1"
                     /translation="MNYSATIPVYSVLNTKVLILNRSYLPIHVTSVRRAFTLLYQGIA
                     KAVNEQYQTFDFDSWSDLSVTVHDESVGMVNRVIRVPRVILLVSYDRMPKRQVRFSRY
                     NIYARDKCTCQYCGQKRPRHELNLDHVIPRTQGGTSTWENVVCSCQECNRLKGGRTPQ
                     QAGMTLARRPVRPQWTPFMQETFSLSRYKEWLPFLNTVDASYWTTELLEK"
     gene            25134..26645
                     /locus_tag="VF182_05775"
     CDS             25134..26645
                     /locus_tag="VF182_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX6436619.1"
                     /translation="MKQEIFINSSPQESRIAIMEDGALAEFLIERKEERGIAGNIYKG
                     KVERVLPGMQAAFVDIGMDKAAFLHASDFFSVPEDVQLISPSGDDVEFDDVPKPAPSR
                     RLPLEKQVSRGEEILVQVAKDPLGTKGARITSHVSLPGRYMVFMPSTKHIGISRRIES
                     EEERKRLKEIAQSLGTTEGGFILRTASEGQSKREIQRDLRFLTKLWKRIQEKAEKAPA
                     PSLIHQDLDLITRMIRDFFTTDTEQVVIDSAKDHRRIADFVGHFMPRLKSKLVLYSEK
                     EPLFDRHGIEEKIEKALDRRVWLRSGGYIIIERTEALTAIDVNTGRFVGKRDQEETIL
                     KTNMEATSEVVRQLRLRNLGGIIIIDFIDMEKEGNRKKVYEALKNALKKDKARTNILK
                     ISELGLVEMTRQRTRESLENQLLSPCPHCDGRGRIKSSVTIAYDILRAIKKQQASLEN
                     GKNIVLRLHPAVANFLYDEKANSLEALEREINHKIIIKVSEDLRHQQYEISTT"
     gene            complement(26648..27331)
                     /locus_tag="VF182_05780"
     CDS             complement(26648..27331)
                     /locus_tag="VF182_05780"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX6436620.1"
                     /translation="MNSFEGNLELSRMDPQNLLEVIQTRRSVRVYKKGKVTDQQLELI
                     LEAARWAPSGANTQPWELVVTRDREKMRRIRQIYDNEWRQRKREDPINYKGLKKDYVG
                     DVSVIVLVCGDARTKQVYLTTRQPGDREKLFQASIANAVQQMMLAAASMNLGTVWVSV
                     REEVEPELRELFKVPTSLRLLWVVPIGHARSWPAAKPRRKPTAFTHLEVYDSSKLRKE
                     SEIRPWPKS"
     gene            complement(27419..29161)
                     /locus_tag="VF182_05785"
     CDS             complement(27419..29161)
                     /locus_tag="VF182_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6436621.1"
                     /translation="MKRRLLRFAARLDQRGQTSSALLSVALVPMAAILALVLVMFWVS
                     VQKGAVGTPGAIYTLANYLEVFSDPFVLRVAANTIIFTATATMVALIFGLPIAWLAER
                     SDLRGKTLIYAIMTLGLLIPGIYTAMGWTLIAHPRIGILNRWLVDLFNLDDAPINIAT
                     PIGMGFVQGLSLSALAFILTAQMFRAMNPSLEEAARVHGMGYGKILWRVTLPLARPGI
                     LAAVIYILTIGIATFDIPAILGLGNRVYMLSTFIYLKVHPQGAGLPEYGITGVVGAFM
                     IIVAGLLTLWYSQVLRQGHRFEVVTGKGYKPALLPLGKWSIAAWCFIAGYTLMSKLLP
                     LLLITYAALTPYFVPPSIDMLGKLSFSHFYGMDWELVMRGLANTAILVAVVPLAVLLL
                     AFSISWLIVRSRSRARYALEFGAFLPHALPEVILAIGALLLSLFVFGTRIPLYGSVWL
                     IAVVYVVARLAFATRALNGSLLQIHRELEEAAFVAGLSTIRTAWRVLFPLIRPTLFSV
                     WIWSALLVYRELTVAVFLAVHDNITLPAVIWSYWYSGSINKASAVTLLMTVVLAPLIL
                     AAWWYGRRSQISAH"
     gene            complement(29374..30477)
                     /locus_tag="VF182_05790"
     CDS             complement(29374..30477)
                     /locus_tag="VF182_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6436622.1"
                     /translation="MIAHQSTPRLASWALTVMVTLATMAALAEAETLTQIIEGAKKEG
                     MLRGQWGQNSFGGSEGFKEILAGMNKKYKLDLKGQYTPGPDMQRLMLRIIQENAAGQP
                     ASTDVYLGNAQAMFDGLKANLLRPMTYNAFIEEKPKSEGKFNAIAADGTHVAVATAVV
                     GIQYNTNLVKGADIPRRLEDVLNPKWKGKIASTPYAAGMREFATPDFLGREKMIDFTK
                     KLSKQIGGLMRCGEAERITSGEFLMLAFTCGGNDVNVLKRAGAPLGHTVIEEGTVLHM
                     RYAAVPKNSRSPNAGALLINYLMSAEGQELLWKHDGLDLHLFPGSQTKTELDKVRAAK
                     GKVIISSPEWLASFKNYAETQKELEAILRDAGK"
     gene            complement(30523..31599)
                     /locus_tag="VF182_05795"
     CDS             complement(30523..31599)
                     /locus_tag="VF182_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6436623.1"
                     /translation="MISIRRLHKRFDVSDGVIAAIRSLDLEIAEGELFVIVGASGSGK
                     TTLLRCLAGLETPDSGEIRIGDRVVSSDNPPTWVPPQQRKLGMVFQSYAVWPHLSVCE
                     NIALPLAEGSQRIPRPEVAKRVKEALRLVQLEEQADRPATLLSGGQQQRVALARAIAV
                     NSQIVLMDEPLSNLDARLREEVRGKIRALAKQLGSTMLYVTHDQIEAMAIADRIALMQ
                     AGELLQVGAPLEIYHKPNCADVAEFFGSVNWLDGHMIEPGIADSAIGRLRIESSSKIG
                     DKVLLGFRPECLSLLDPGAHFDGNSFRAKIGSSTFLGDQFVYTAVVNDRQLFGKSRGV
                     PESSDGTALCHVSSSDIMVFPARM"
     gene            complement(31772..32281)
                     /locus_tag="VF182_05800"
     CDS             complement(31772..32281)
                     /locus_tag="VF182_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6436624.1"
                     /translation="MADEQSPIKIKKIGHVVYTVSDLERTSKFWTEIMGFKFSDRNEA
                     GMVFFRNASDHHTVALMQAKENTPLPKRGQVGFDHFALEVATVSELFKIRDFLKAKGV
                     TIMYEGRRGPGGNPGVEFLDPDGYQIEIYASMDQIGEDGKSRPASEWRRATTLEEAVA
                     NPLPGAEYH"
     gene            complement(32523..32894)
                     /locus_tag="VF182_05805"
     CDS             complement(32523..32894)
                     /locus_tag="VF182_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008881216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HEX6436625.1"
                     /translation="MSHFWRIAEERIKEAQRAGAFDNLPGKGKPLDLEDLSWVPEDLR
                     IGYLVLKNAHVLPPEAELLKDIHTIEDLLKHVEDEGERRALAKSIQFKLIRLDMLKRR
                     SMPLRSVRDYSRKLVDKLRTK"
     gene            complement(32891..34138)
                     /locus_tag="VF182_05810"
     CDS             complement(32891..34138)
                     /locus_tag="VF182_05810"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6436626.1"
                     /translation="MFKELHHDAKLLMASRGIRSFAFSYLNVVFAIYLDQLGYTPVMI
                     GIIFSVAYLSGAVLTAVWGYLSDRYGRRRILMLLAVLTIVSNSIFIFFSGLVFILLAV
                     VIANVGAGGAGGGGSGGGPFNPVEEALLAEKCKPENRNQVFALNSFVGSIMGSLGALG
                     AGLPQYLQQIRGWTAVSSYKPLFMLTIIFSVVLFYVYSQIREEHQPRKTERKKISKAT
                     GVFVTKLSLLSFVDNFGAGLAGSLVSYWFFLRYGVELKSLGLLFFISYFLAALSFLSA
                     PIIARYIGLVKTMAFSHGLASVIYLLIPLAPTFKIAATMLAVRSYFAYMDNPLRSSFT
                     MAMVRSSERGSAAGVTSLARIVPFGISPTISTYLMQSVSLTLPLFIGGGLQLINDVAF
                     YLMFRHIKPPEEITEATVERSPA"
     gene            complement(34146..35105)
                     /locus_tag="VF182_05815"
     CDS             complement(34146..35105)
                     /locus_tag="VF182_05815"
                     /inference="COORDINATES: protein motif:HMM:NF018349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1152 domain-containing protein"
                     /protein_id="HEX6436627.1"
                     /translation="MSSGSPSLEDIIGESSRSLVIGIGGGGDVAGALATARFLEFCGL
                     EFVLGGLSWERRVFDPLPGPRMLSEVDNVRALHQYAWMANRETQSKAAVYFAESRMAE
                     FLAQEVLLVDINGGVRGVVAGLERAIEVLQTDLLVGLDVGGDSLAKGTEPGLRSPLAD
                     SMMLAAFVELEKRGYRALWGVFGYGSDGELTIDEIEAALTALAGAGGLLGAWSLTPKT
                     AAELEAVIKTVPTEASAIPVQGFRGVSGEREIRDGGRRVKLTPLTALTFFMSATKVFE
                     TLSRPAQAVSGSSSLDQANDALHAIGIKTELDAERECYQAQKA"
BASE COUNT         7917 a         9417 c         9223 g         8557 t
ORIGIN      
        1 tcgttcttta atctgcttct cgtaaccaga ctctgacggt tcataatatt tttgtgtttt
       61 cagttcatcc ggtaaatgct gttgatcgac tacatgatcc ggaaaattat gggcatactg
      121 ataatctttg ccgtatccca gttttttcat cagatgcgtc ggggcattgc gcagatggag
      181 aggaacaggc agagcgccgc attgctcgac atcctgcgcc gctgccagca tcgccaaata
      241 ggaggcattg gatttcggcg ctgtggctag atacgtcacc gcttgcgcga gcggaattct
      301 accttcgggc aggccgacga aatgaaatgc atccttggcc gcgacggcaa cttgcaatgc
      361 ggcgggatcc gcattgccga tgtcttcggc ggcaaagatg accatccgac gcgcaatgaa
      421 caacggatcc tcgccggctt cgatcatgcg catcatccaa tatagagcgg cgtcagggtc
      481 gctgccgcgc aggcttttga tgaaagccga aataatgttg taatgctctt cgccggactt
      541 gtcatatcgg agcggctttc gctgcagcgc ctcttgtagc tgtgtcaggc cgatctcttt
      601 ggccttcgcg ttatgcgcca aagtcgcggc ggtttccaac gcattaagcg ccacacggca
      661 atcgccctgg cattgatcga tcaaaaactc tcgaccttcg ggcgagaggt tcaaatgcat
      721 gttccccaag ccgcgttctt tatcttgcaa ggcacgatcg acaatcgcgg cgatctcctc
      781 ggtcgataaa gctttgagca ctaaaacctg ggtgcgagaa agcaggggag agatgacttc
      841 gaaagatggg ttctcggtag tagcgccgat caaaacgatc gtgccgcgtt cgacgtacgg
      901 caaaaaatta tcttgttgcg atttgttaaa atgatgaatt tcgtcgacga agagaaccgt
      961 tttcttgccc atacgcctga gccgttcagc ctcgtctatg atcttcttca ggtccttgac
     1021 accggaggag acggcggaaa actgcacgta aagcgcgccg gacttgctgg cgaggatttg
     1081 cgctaaagtt gtcttgccgc tacccggcgg gccccaaaga accaatgatc tgagctgggc
     1141 gctctcgacc atctcggtta aaattcgtcc cggtccgagg aggtgcttct gcccgacgaa
     1201 ttcatccaag tttctcggcc gcatccgttc tgccaggggt gctcgcgctg attgttgctt
     1261 tgactgatcc gattcaaaca aatccatgat gatatgctag cacaaccgtg gggcgccgtt
     1321 gtacccggcc aattcaaacg attcggtaag acagaatcca agcacaagct tgcggcattg
     1381 agcctgctct gagtggcacc gctggacgtc aattgcgaaa cgtcggtttc actttcggtc
     1441 gtcgtccagg ggtgatattc tagctgtcgt ttaggattgc agctatggcg gaaaaatcag
     1501 aaagcaacgg tagctctccc ccccttgttc aaggaacggt gcgcacagtt ttatttccgg
     1561 actcggcgat atcggggaga ctaagctatg atctgccggt taagatcgat agcaagttta
     1621 ctggagaggt taaggccagc gatcttttgg tggtcggcgc taatgcgcac gtagaagcac
     1681 gaattgccgc gcgcaatctc cagttagagg gaaagttgat cggcagtgtt caggtaatcg
     1741 gttgcttcga gatcatgccg ggaggacatt tccagggtga tgttcaggct gcagagctca
     1801 aggttcatcc cggcgcgacc tttgatggtc aagggaaaat aaccggcgag ccctaagatt
     1861 gcgtttagcg gttgccggac cgttcgtctg aaaagagccg agccgccttt ccggatctat
     1921 cgcgcaaata ataaagtttt gcacgccgca ctctgccgcg cgaaaccact tcgactttat
     1981 cgatacgtgg ggaatgaaac ggaaaaatac gttcgacgcc taccccgtac gatattttgc
     2041 gaacgacgaa attggctcgg tttcctttcc cgccaatgcc aatgacaatt ccctcaaaca
     2101 cctgagttcg ttccttttcg ccttcgacaa ctttaacatg cacccgcacg gtttccccgg
     2161 ctcgcagcgc cggaatttcc tgtttggatt gcagggtttc aagtttgtct agaatattca
     2221 tggctactcc ttaaagctaa cgtcccagaa gacggtctaa aatgatcgct gctgccgatc
     2281 gaaccgagag atggttgtaa tcggtccatc cttcgaccgc atccaaaaga taatctgatt
     2341 gagagattat tgtctcagtg agtccccagc ccgtgcccaa aacgatcaga aaagggtgag
     2401 tggtattaat taacatgtct tggagctcgg cgaaagaggt cctctgacct ccgggtctcg
     2461 cggacgtcac gacaatacgg ggtttttcac cgtattcttg ctccaaatcg atgagcacat
     2521 catcgagcgt gtttttaaca cggacgagcg cgagggcctc ttttcgcgtc gtgttatatt
     2581 ggctgccata gccgtgctcc caatggtcga taatcttgag cgcgagtctt tgaagcgctt
     2641 tgaccggggt aaccacataa aatccttcga tgccgtatgt tcgtcccgag cgtgcgatat
     2701 catggatgtc catgttggta accgctgtag tcacaatctg ccggttctta tcgtagacgg
     2761 ggtaatgaac cagggcaacg tagacaggcg cccgttgatt acagatcagc tccttttctt
     2821 cggccgacag ctcggcgctc cggatcaagt ccggccgtcg tttccaggtt tgctgaatgc
     2881 tcatgtgacg ccgccattgc cggatccgtt catggtcgcc tgacagcagt acatccggca
     2941 ccttggcacc gtggaactcc tctggccggg tatattgagg atattcgagg agcctattgc
     3001 tgaaagactc atcggcggca gatttggcgt tgcccaacac gccagggatc aaacgggata
     3061 gggcatcgat gacaacggta gccgcgggct caccgccgct aatcgtgtaa tcgccaatcg
     3121 aaatctcttc atcaacgaaa cctttgaccc gctcatcaac cccctcgtag cggccgcaaa
     3181 tcagcacgag ttcttcttct ttggccagct caacggcgac ggcttgattg aagacacggc
     3241 cctggggcga tagaagaatg accttggatc gcggcatttt agctttaacg tcctcgatcg
     3301 ctgtcaccag cggctccggt ttcatcacca tgccttggcc cccgccgtat ggataatcat
     3361 cggtcatacg atggcggtca gtggtatatt ctcgtggatc gactaaaaca attgaaatca
     3421 aacctttctc ctgggccttc ttcagaatgc tgtggttcaa aggcgaagca aacatcgccg
     3481 gaaaaagagt aacgatcgta aaggtgacca tggaataggg aggacgaggt tcttagagat
     3541 ccaggagacc ggcaggaaga tcgacgatga ctcggccggt tgccaaatct acttcggcga
     3601 tgatttcctt tacagccgga atgagatgtt ctttatccgc cccggcgatg acatacaact
     3661 cgctagccgg tgttgaccat atttccttta aggttccaat tcgtgtgccg ttgatgtcga
     3721 agacctcgag gccgattact tggtaatgat agtactgacc gggcgccgga atctccaacg
     3781 cctcttcggg cacactgacg gtcgatccca cccaacggct cgcgctttcg atatcatcgg
     3841 tgccgcagag cttaagcaaa atctgtcgct tatgcgggcg cgccgattcc acctggacaa
     3901 cggtgcgggt ttcgtcctga acgaggatga cctcgttaag agttgatagg atagggctct
     3961 cggaattgta cggattcagc ttaagccatc cggcaattcc gtgggttgtt acgatttgcc
     4021 ctaatgggac gattccgtta ggcacgcggt tagcctaaca gactggctat ttttcttcta
     4081 cgatttccag gacaactttg cggttggttt ttgaagctgc ggcattgagg atcgcgcgaa
     4141 ttgattttgc ggtccggccc tgtttgccga taatccggcc caagtcatcc ttggcaactc
     4201 ttaactcgta aacggctgcg tcctctgccg cgctctctgc tacttcgacc gcgtccgggt
     4261 tattcaccaa agacttcgcc aagtattgaa cgagttcctt catgggtggc gctagtggcc
     4321 aaaagttagc tggctttctt ttgttttgcg atcagctggg aaacggtttg tgtcggctga
     4381 gcaccgcgct tgatccaggt ttcggctttc tcttgattaa tcttaaatgt tcccccccct
     4441 gcggatggat catacgtgcc gatctgctca atatatctgc catcgcgggc aaaacgttgg
     4501 tcactgacga caatccggta gaacggtttt ttctttgcgc catgccggct caaacgaatt
     4561 cttacactca tattcccttc tcctctagtg aaacgggttg gccatgcggc taaagagcga
     4621 tttcattccg cccttgctca cccgctgcat catttttttc atttctaaaa actgtttcat
     4681 caaccgattc acatcagcta cgctggtgcc gctgccttgg gcgattctgc ggcggcggct
     4741 gccgttcaag atcgccggac tgcgccgctc ctgaagagtc atggaattga tgatagcttc
     4801 gacccgcttc aattcccttt cggctttatc ggcatcgacc tgagaggcta gcttcttgcc
     4861 gcccgggata agctcgagca ggcttcccat ggatcccatt tttttaattt gctgcaattg
     4921 aatctgaaac tcttcgagcg tgaactgttg cttctgaatg accttttgga ggcgctcggc
     4981 ttccttctgc tcaaaatttt gttgaacttt gtcgatcaag gagaggacgt cgcccatgcc
     5041 gagaattctc gatgcaagcc gatctggata gaacggctcg agtgcatcga gcttctcgcc
     5101 cgttccggca aacagaatag gcttgcccac cagctgtcga atcgataaag cggcgccgcc
     5161 gcgtgcgtcg ccgtccagct ttgtcaggat gacaccgctg acgcccagct gctggtcaaa
     5221 acctagcgct tgattaaccg cgtcttgacc ggtcatggaa tcggctacga acaaaatctg
     5281 gtgcggatta acggtgcctt tgatctgttg aagctcttgc atcagctctt cgtcaatgtg
     5341 cagacggcca gcggtatcaa tgagcagtaa gtcacagaag cgctttcttg cctcgtcgag
     5401 agcttgttta cagatggcaa ccggcgaagt ctgcgggttt gaatcgtaca ctggcatctc
     5461 gatttccgcg ccaagaatct taagctgttc gatggcagcg gggcgataca cgtctgcagg
     5521 aacgagatag ggagttcgct tttcatgttt ttttagatgc ctggcgagct tggctagcgt
     5581 cgtggtttta ccggagcctt gcagtccgac cagcataata acaaccgggg gcgacgcttt
     5641 gaggtcgagc ttacgatgat caccacccag gaggccggtc agttcgtcac ggacgatttt
     5701 taggaactgt tgctcgggag ttagacttag aagaacctct tgacccagtg atttcgcttt
     5761 caccgaatcg aggaaggtct taactacttt cacatggaca tcggcctcca agagcgccaa
     5821 gcggagctcg cggagggcat catcgatatt tttctcgctg attttccctt cgccacggag
     5881 ccttttgaac gtgagttcta gtttttcggt caacgactcg aacatcgctt gaatcaccta
     5941 agctatgata ttaacaggag aaagtaacgc tcgaacgtaa tacgtaccga gccggttgtc
     6001 aagaagagct aaaaaacaaa ggcccgttat gccgtgccac ggcataacgg gcctggatcg
     6061 tgacagactc tctacttaca tcatgccgcc cattccaccc attccaccca ttccacccat
     6121 cccacctggc ggcatcggag gcatcgctga cttctcttcc ggtttctccg ccaccatcgc
     6181 ttcggtggtt agaagcatac tggcaactga agccgcgttc tgtagagcgg tgcgaaccac
     6241 tttggtggca tcgatgatac cggccttcat taaatcctcg tactgttcag tcgcagcatt
     6301 aaagccgaaa gcacctttat tctcttttac cttctgcagc acgatggatc cttcgacacc
     6361 tgcattcatc gcaatgcgcc gaatcggttc ctcagaaact cgtttgataa tattgacgcc
     6421 ccagcgctcg tcttcatcga cgcttatttt atcgagccct gcggcggctc tcagaatggc
     6481 cacgcctcct ccaggtacga tcccctcttc gatcgcggcg cgagtcgcgt gcaaggcatc
     6541 ttccacccga gccttctttt ctttcatctc aacttcggtg gcggcgccta cgttgatcac
     6601 agccacgccc ccgatcagct tggcaagacg ttcttggagc ttttcgcgat cgtaatccga
     6661 tgtggtctcc tcgatttgag cgcggatctg tttgatacga ccttctaggt cggccttttt
     6721 gccggcgcca tcgacgatgg tcgtgttgtc tttgtcgaca acgatcctct tggcgcggcc
     6781 caagtcttga agcgcaatat tctcgagctt tacgccgagc tcctcggcaa tcagcttgcc
     6841 gccggtgagg atggcgatgt cttccagcat tgccttgcgg cgatccccga accctggtgc
     6901 tttgacagca acgcaatgga gagtgccgcg aatcttgttg accaccaacg tcgcaagagc
     6961 ctcgccctcc acgtcttcgg caatgatgat aaatggcttg cccgatttcg caatctgctc
     7021 gagaactggc agcagctctt tcatattgct gattttcttc tcgtgaatca gcaagtaggc
     7081 gtcctcgaga atgcattcca ttcgttccgg atcggtcaca aagtagggtg ataaatagcc
     7141 ccggtcgaac tgcatacctt cgacgacgtc caaggtggtc tccaggcctt tggcctcttc
     7201 taccgtaatg acaccttctt tgccgacctt gttcatggct tcggcgataa catcgccgat
     7261 ggtcgaatca ttgtttgcag cgatcgttcc tacttgggca atctcttttg gatctttggt
     7321 gggtttggat aaacccttca gctctgcggt gaccgcttca acggctttgt ctatgccccg
     7381 cttcagagtc atgggatcat ggccagcagc aaccagcttg acgccttcgg caaagatctg
     7441 ccgcgccaga acagttgccg tggtcgtgcc gtcgcccgcg acgtcggagg ttttacttgc
     7501 aacctccttg accatctggg cccccatgtt ttcaaacttg tcttcgagct ctatctcttt
     7561 ggctaccgtg acgccgtctt tggtgacatt gggtgccccg aaggatttgt ccaatactac
     7621 gtttcttcct ttcggcccta acgtgaccgt gactgcgtcc gctagaatgt tgacgcccct
     7681 caacatcgcg tctctagcgt cgcgattaaa ctttactatc ttagatgcca tcctttttcc
     7741 tcctgagtta gatttggtaa tttacttttc gaggacgccg agaacatcat cttcgcgcat
     7801 gattagatgt tcttcgccgt ctatatttat ttcgcttccg gaatatttcc caaagagcac
     7861 ccgatcattg actttcacat ccaaaggggt aattttgccg tcctcactta cctttccctt
     7921 acccacggcg acaacttttc cttcctgcgg cttttccttg gcggtatccg ggatgatgat
     7981 gccaccctta ctcttctcct cctcctcgat acgtttcacg atgatgcgat cctgcaaagg
     8041 cctaattttc accttttgcc tcctttcgtt ttaactgagt tgggttctga tcgggcggtc
     8101 cggttgtgca taagccgcaa atggaccgtc aaagtgtgac caatctaagc acggatcccg
     8161 acctgtcaag gtaatccggg cgaaaaagtt gattattgcc gatgacaaga gatctatgtc
     8221 atttgagttt ctggcgtctc atctcttctt cgagatactt acgatacaac acgtcccatt
     8281 ctcggcttcc cggtggaatg tgccgggaga gagactggat tttctgtcgg accagatcat
     8341 caaaccgatc ttcgcgttca aagaactcgt gtaggatgcg tttcgtttca gaaagcgctc
     8401 ggccctcatt agggaactcg gccacgtcgt cgcggttgag tccgctaacc accagatggg
     8461 ccagatgcga gatccggctc gcggagagcc ggctcataga ataaagccct tcttggctgc
     8521 caacttttgt ttggcgatta gaaacatttt gaaatggtcc atctcccgag ttgcctgaga
     8581 atgagcggcg agcatcttgc gcgcctcgtc ttcgatagct tcctcttcgg caaagttccg
     8641 gctgattaca cccctgatct tggcgaggat tatcggatcc tcccgtttcg gccgtattaa
     8701 ctcctttgcc cgatagtttt caagaagcag cctggcgagc ttatcgagct cttccggttt
     8761 tattttcatg ctgtgaaaaa cctgttgata agttgcgaac agctagtgcg aagatccggt
     8821 agaagcagtg gaccaaatga tttctatccg cgttagtgat cgatgtcaac ccgagccatc
     8881 gccgcactcg accatcgggg tgtcgacatc cgtcgggaaa aaatagcttg tgaatttaag
     8941 ggttttttgt cactatgagc ggtgctcatg aggttttccc gcccaggact ctatcattga
     9001 tgctgactta tccacagccc aaattcatgc ttatttaagg tgcctttcga gaggttgtga
     9061 ataactaggt gatcatcaaa aaacccccga gattacggcc aggacaccgc ataggtatta
     9121 ttgcgcccgc cgggtgcgtc gagcgagatg ctctgtcgag gggggttcag gtgattcgag
     9181 atcagggatt cgaagtcgaa ttgtcccccc gaatttttga caccaagggc tatcttgccg
     9241 gagacgagcg aaatcgtgcc gaggatctag tcaatttttt ccaaagaaat gatatcggcg
     9301 cgatcttctg cgcacgaggc gggtttggtt cagcacaact cttgcctttt ttgatacatt
     9361 tgaatttttc ccctgttccg aaaatttttg ccggctatag cgatgttacg tttctgctga
     9421 actggatcca cagccggttt ggcatcgtaa catttcacgc gccgatggtt gcgatggagt
     9481 tggcgcgtgg ccttaggggt cgtactcaag aagctttctg gggctccgtg accggcgaca
     9541 aagcggagtg gcaagttgag ttgggcgagg tgatccggcc tggaaaagct gaaggcacga
     9601 tgatgggcgg ctgtttatct gttttggtga caacgctagg gacgccttac gagatccaaa
     9661 ctcatggcaa actactattt ctggaagacg ttggtgaacg gccttaccga atcgaacgaa
     9721 tgctgacaca tttgaaaatg gccggaaaac tggatcacct cgccgggctg gtttttggcg
     9781 acttcagcaa ctgcgaagcc gagggatcga ggagcgtgcc gcgaatcatc aatgagatgt
     9841 tcgcggaggc tccttatccc gtattaacgg gcttgccggc tggacatgga agcgaacaca
     9901 tgaccctgcc attcggtgtc aagatgatac ttgacggaaa cactcgatca ctttcgctga
     9961 tcgagtcgcc agttgcttga aagcatttct gcaccggtgc gatttgcgcc cgagagccat
    10021 tcgatgacca tggactttgc tgcgattgag gatgcgaccg aagaagcggt caagcagggt
    10081 gtgtttcccg gcgccgtact gctggtcggt ggtggagaag atgtgatcta tgaacgagct
    10141 tttggctggc gcagtttgct tcccgatcgc actcctatgc gctcgtcgac gattttcgac
    10201 ctggcatcgt taaccaaacc tttagcgacg gcgcttggaa tcatgctcct ggtcaatgag
    10261 aacaaactcc aactcgacga tcaggtaact caatttgtgc cgagcttggc aaacacccca
    10321 acaaagatcc ggcacttgct aaatcataca tccggcctgc ctgcctggaa accctactat
    10381 gaagaggtga tccactgcgt cgatagtgga agaaccggct tcatcggaag cgcgggggcc
    10441 aaaagctaca tcttgggaca gctccatcgc gaagcgctgt taagcgagcc gggctcgcaa
    10501 gctctttaca gcgatctcgg attcatgctc ctcggcgaaa taatcgaaaa tatcagcggg
    10561 acaaatttgg atgttttctt tcatcaccgt gtgtttcagc cattgaaatt acgctcggca
    10621 acttttttca atgtggcgat ttctccaaca tcgagaatcc aaaacgaaga ggatgaaatc
    10681 gctcctacag aactttgccc gtggcgcaaa aaagttcttt gcggcgaagt gcacgatgat
    10741 aacgcttttg cagccggcgg cgtggccggg catgcgggtc tgttctcctg tgcccgggac
    10801 attcatgcca ttgtcgctaa gttgaatcgc tgcctgcaag gttgcgattc attcctgtcg
    10861 caggagttgg ttcaggagtt ctttaccaag gacacttccg tacctggttc tacgcacgcc
    10921 ctaggctggg atactcctgc gccggagaag tcgtctagcg gaatgcattt ttcccgcaag
    10981 tctgtcgggc accttggctt cacgggcacc tctctctggt gggatttgga gaagaattgt
    11041 cacgtgattc tgctgacaaa tcgcgttcac ccgaaccgaa acaatgaaag aataaaacag
    11101 tttcgaccga agattcacga tctgattatg cgggcaatca atctatgaac caactaccag
    11161 ccaaaggaag ccacatacat ttaattgccg tatgcggagt aggcatggcg tcattggccg
    11221 gccttctcaa ggctcacgga taccgcgtca ccggctcgga tcagaatgtc tatccgccga
    11281 tgagcgccta tttggcgcaa gtcggcatcg acgtacaaaa tggatatgac gcgcaacatc
    11341 ttgccgaccg gcctgacttg gcgataatcg gcaacgctgt ttcacggggc aatcctgaag
    11401 cagaagctgt tctggacagc accattcctt atctttcatt cccgcaagct ctcggaaaat
    11461 ttctgatcgg tacgcgccag tctatcgttg tcacaggaac acacggcaaa acgaccacga
    11521 ctgctctaac tgcttgggtg ctgaaatgcg cgggcctcga tccgggattc tttgtcggcg
    11581 gtgtgccgct caatttcgcc agcggctggc acccaggaga aagcgatcag gtgattttgg
    11641 agggcgacga gtacgacagc gccttctttg ataaaggacc caagtttctc cattaccggc
    11701 cgcaaagcgt cattcttacc agcgtcgagt tcgatcacgc agacatttat cgggatttga
    11761 atcacttgaa ggaagctttt cgatctttag cgcgactcgt ccccgccacg ggactgctgg
    11821 ttgcctgtta cgattatccg gcagtgcgag aagtcgtaaa agccggttcc tgccgggtgg
    11881 tatcttatgg cgatgacgga atctgtgatt ggacggtgcg cgatgtccga agcgaagggc
    11941 ggcggagtta ctttgatcca tgttttagga acgcttctga gggcactgtg gagcttggcg
    12001 cgatcggtcg gcataacgtg aagaatgctt tgggtgttta tgtcttggct cgagaattgg
    12061 ggatggagcg gaacaatctg ctcgaaggtt tcgcgtcttt ttcaggtgtt aaacggcggc
    12121 aggaattcaa aggagagaaa cggggaattc tagtcataga cgactttgcg catcatccta
    12181 ccgcggtcgc ggaaaccatc aaagcggtta aggacgcctt cagcgggcga cgactctggg
    12241 ctgttttcga accgcgcagt aacaccagcc ggcgcaacgt cttcgagcgg gagttttcgg
    12301 tggcattgtc actggcagac cgggtcgtcg tctccggtct tcatcaaccc gacaagattc
    12361 ctgaagcgga gcgattatcg gtggagcgtg ttgtcgatgc tatcaatcag tcggccggac
    12421 gcgagcaggc ggtagtaatc aacggcgccg acaacattgc cacccacgtg tctacccgcg
    12481 ctaccgcggg ggatatcgta ctggtgatgt caaacggcgc gtttgacggg gtgcaagaca
    12541 agattttgtc gggattagcc agttaggtgc aacgcagcgc attcctcact gcaaaaataa
    12601 tttcctttgc ggaaaagcgc ctcgctcttt ggaacatagc tctggcagtg cggatccaat
    12661 accatctctt ctcccctaga tctggcgagg tttggattac gcagaaagct tttacgagcg
    12721 agaaaatagg cgcgcagggc attaatgaca acccatgcga cgacgacaaa tacgagcagg
    12781 cgcaatagca ctgtgaatta tctaacagga cccggctcga ctctcaaggg tgaaagagat
    12841 ggattttttt ttcgtctttg acattgacaa gtagctctga gatcgtgacc ccaagctcgg
    12901 ggtgaattac ttgcggggcg atctcgctca gcggtattaa aacgaacttt cggttaggca
    12961 tctcggggtg ggggatgtgc aagttttttt tcttcatcac caatccatcg tagagcaata
    13021 tatccagatc gatgatgcgc gcgccccaac gtttcttgac ctttttgcgg cccatggccc
    13081 gttcaatgtt tttgaacttg ttgagcagca tctctggttt gaatttcgtt tcaatttcaa
    13141 tcgcgccgtt gacataccaa tctcttgaat cccctaacgg ctcgctttcg taaagagacg
    13201 attctttcag gatttttgta tcgggaagct tggccacgcg gtccagagcc tcaaggaagt
    13261 tgtctttctt ttggccaaca ttggagccaa tcccgatgta gacctgatgt gctacggcgg
    13321 ctgccatgac gcttggttgg aaaaatccta aagattgact tgccggattc tttccgtgac
    13381 ctccttcaaa cgttgctgat cgacacatac cgtgaagcga acataaccct cacctgcatc
    13441 gccaaagccg tttccggggg ttgtcacgac gcccgcttcc tcaagtaact tggcggtaaa
    13501 ttcagcggaa gatagaccct tcggagtagc cacccagacg taaaaagtcg cgcgaggttt
    13561 gtcgcattcc aatcctatgg cgcgcaatcc agaaacaagt atgtcgcggc gctcttgata
    13621 aatctttctc gacggctcaa tgatgctgtc gcccagttgc agcgcttcaa taccggcttc
    13681 ttgtatcgcc tggaaaatac ctgagtcgag gttggacttc gcttgtgcta atccgctgac
    13741 aatttccgca ttcccgaccg ccattccgat gcgccacccg gtcatgttga aggtcttgga
    13801 taatgagtgg aattcaatac cgacctctct tgccccttcg aattcgagaa aactcatcgg
    13861 ccgatatccg tcataaccca tttcgctgta ggccgcgtca tggcatacga ggacattgtt
    13921 cttggtggcg aaatcaatga ccctttcgaa aaaactacgg tcggcgacgg ccgcagtcgg
    13981 attgttggga tagttgatcc agaggagttt ggcctgtttg gcgatttcac tagggatcga
    14041 atcgagatcg ggtagaaact ggttctcttt tttgagcggc aaaaaataat ttctcccgcc
    14101 gctgaaagca gtgccgatat ggtagaccgg gtaacacgga ctggctacca ggacaatgtc
    14161 ccccgggtcg acgtaagcta ccgccatgtt tccgatgcct tctttcgagc cgatcagaga
    14221 cactacttct ttgccggcat tgagttttac gccgaagcgt cgctggtacc aatttgccac
    14281 ggcttgacga aactccggca gccctgaact gttgggataa cgatggttta ccgggacgct
    14341 ggctctttca atcagtttct cgacaatcgc cgcgggagta ggaatatctg gatcgccgat
    14401 gccaagatcg attaggtcaa caccccgggc taacgcctca cgttttcgcc ggtcgatctc
    14461 ggcaaacaaa tagggtggta aatcgttaat ccgcttggct tttttaatca gaatgggcaa
    14521 attacgtgaa cctccatgct aaaatcgcac tggttgtacc tggtttttgc aattttgaca
    14581 accatttcaa agcttcttta gtgatgagtt tggtcaagga tatttcatgg acatcatcgt
    14641 agacgggtac aatttgatcg gcagcgagcg cggtttgact ggcggacttg agcaccaaag
    14701 aaactggctg cttcaccggc tttcgcatta tcaagaactt agaggccata ctgtggtctt
    14761 ggttttcgat ggctggaagt caggattaac ggatgaggtc tcgcaagata agcaaggagt
    14821 ccaggttgtt ttttcacggc agggagagaa ggccgacagc gtcatcattc ggctcgcgcg
    14881 ggcaaagggg agcggatgtg ttgtcgtgac ttccgatagg gaagtgcgca gagccgtgga
    14941 aaaattcggg gcagcggcaa tttatgcgga tgagtttacc cgaattttac gcaatctcga
    15001 tttagcggat gccggcagcc aggaagggga gcgccggccg aacaagaagg gtaacccaaa
    15061 gcggcttgcc aagagcgaac gacaacgtct aggaaagctg aaaaaactct aatccaggta
    15121 agccgcggga tcaatgctcc agtttctttg tcgtttctat gcgattgacc gcgccgttgc
    15181 ggaataccag cagagtgcca tcgggataga accagtgctc ctcattggca tgttgcggac
    15241 cttttatgcg acttacccgt cgcggatcgc cgagaacccg tttgacctca ccggcgctca
    15301 tcccaggcac cgcttggcgt ttgatggctg attccttgac ggccgaggac caaggctggc
    15361 tctgaatcca ttctactcgc gccttttcct cttggttttt ttccgccaaa gcctttcttt
    15421 catcagctcg cgggtcaacg gtgagtattg tacgattgaa ttgctcgtga aaaacctgag
    15481 cctgaatgta cccttccgct ccggattcaa actttacttt gtagtaggga tttctagctt
    15541 tccttcccac cagctccgtt atttcgaagg attcgttatc gctgggctgg aataccgatg
    15601 catcagaaat tggagttgtc acaaaggacg gtagctttcc ttcgaccgcg gctacccaga
    15661 acttcttgcc aactcgcgtt tccaacgcat caagctgctt ttccgtgtag tggtgcgatg
    15721 ccgctgccga tgtgagaaga caaaacacga taagtagaac catagaggcg accagtctca
    15781 taaacgtctc gcgtagcaag cgatcaatcc aatttaaatc caagcccgca ataacgccat
    15841 agatgctgaa tggtctttgc acttatcgtt tattcggatt ttgtactttg cccgaaaaaa
    15901 aggaggctca tggagcctcc tgatatggtt gcggggccgg gatttgaacc cgggacctca
    15961 aggttatgag ccttgcgagc tgccagactg ctccaccccg cgatatactg taggatactg
    16021 gttgtacctt gcgatttatg aggagtcaag gtaggcattg tttatgagtg aaaaacctgc
    16081 aactccattg acgacggtgg atacgattat cgagatctcc agcggcggaa ttgtcttagt
    16141 cgaacgaaaa aatcagccgc acggctgggc cctgccaggc ggctttgtag attatggaga
    16201 gtccctggaa gtcgcggcgg tgcgggaagc caaagaagaa acttgcctcg acgtcacgct
    16261 cgttgagcag ttccatactt attccgatcc gcacagggat ccgcgtcatc acacggtgtc
    16321 aactgtctac ttggctatcg cggacggcgt tccagaagga ggcgatgatg ctcaagatgc
    16381 caaagtattt atcgaaaacg aacttcccga tcggatagtc ttcgatcatc caaagatcct
    16441 cgcggattac ttcaggtata agaaaaccgg acagcggccg aagccctgag aacatcaaga
    16501 cgactggatc ttgccaaatg cctgccgcat gcgccggcat ccctcttcaa cattctctag
    16561 tgacgtcgca tacgaaagtc gaatgtgcgc gttggaacca aaatcttccc ccggaaccgc
    16621 ggcaacggtt gcttcttcga gcagatagtc tgcgatatcg cacggtgacg ctaatttctt
    16681 tccattgata gtcttcccga tcaacgccgc gatgttggga aacacataaa aggctccttg
    16741 cggctttagg cattctacac cggggattgc gtcaaggcgg tcgacgataa cgtcccttcg
    16801 tttctgaaat tccccgacca tcatcgccac gtcctcctga ggtccgctaa gggcctcgag
    16861 cgcagccgcc tgggctatgg acgtcggatt tgaagtgctc tgactctgaa ttttggccgc
    16921 ggcgccgata atgtcagccg ggccgagcgc gtaccctata cgccacccgg tcatggcata
    16981 ggtcttcgat acgccgttta cgataatgac tctgtcacgc aacgacggcg ctagcgcaac
    17041 cagattttgg atagtgaacc ggtcataaac gattctttcg taaatatcgt cagtaacgat
    17101 tagacagtcg tgtttctcga gaacctgcgc catggccatt agttcattct cttcgtagct
    17161 agccccggtg ggattactgg gactattcag tacaaatact cgggtacgcg aagaaagtgc
    17221 ctccgcgagt tgttgcgcgg tgatgcgaaa cccgctcgct tcggtggtct ggatggtctt
    17281 gggcacaccg ccggctaaca atgccatatc cgcgtaactc acccagtaag gcgctgggac
    17341 aagaacctcg tctccgggat tcacgacggc ctgaaaaaga ttatagaggg agtgtttggc
    17401 gccgcagcta accaatattt cgctcaattg ataactcagc ccatactcgc tcaggtattt
    17461 tcgcgcgatg gcctgtcgca gcggctcaac tcctctgacg tcggtatatt tggtcatgcc
    17521 ttttttcagg gcttcaatag cagccgcttt gattctgtca ggagtatcaa agtccggctc
    17581 cccggcagag aaattgatga catcgatgcc ttcagagcgc agttttcctg ctttggcggc
    17641 gatcgctaag gtgaccgatg gtttgatgag tagagttctt tctgccaacg tcgtcatcta
    17701 ttgttcctgc ctgaacttcc ggttgagttt cttaagaaca ttattgggaa ctagaccttc
    17761 tacgttgccg cccaaaccga cgacctcttt gaccaggcgt gagctgatgt aaaactggga
    17821 ttcaccggtc atcataaaaa atgtttctac gttgggcttg agtcggtggt tcatcatcgc
    17881 catctgaaac tcatattcaa aatccgagac cgcgcgcaat cctcgaatga tcaccggtgc
    17941 ttgcttgcgt tcggcataat caaccagcag cttttgaaag gaatccgcct cgacgcgatc
    18001 ccttaaagcg tcgaagactt cacggatcat ctctaaacgt tcttcgaccg agaatagcga
    18061 cgtatcctta cctgcattac tggcaatggc gacgattact ttgtcgaaga cgcggagggc
    18121 gcgcttgacg aggtcgacgt ggccgttggt gatcgggtca aacgatccag gataaattgc
    18181 aatgttttgt gtcatctcct aggtgtcctc attcggctga gaccatgccg ctttgtgttt
    18241 aaagaacgat agccgggtgt cgccgtattt tcgttgatct tttaatatca gggaaccgta
    18301 ttccgcttcg ggccgctcgc gcgcgctgtg ttcgacaacc agtgttccgg atgctcgcaa
    18361 cagattgccg cgcgcgatga cctctagaca cgcgttaatg aaattgtgct ggtatggcgg
    18421 atcgagaaaa atcatatcaa acgattctcc ttgccgcgcc agcgagcgta gggctcgaga
    18481 gactggtgca atccaaacat tagcacggtc cgtaaaacca acattgcgga gattctcggc
    18541 gattacctta ccagatttgt ccgacgagtc tatcaaaatt gcttccgcag cgccgcggct
    18601 gatcgcttcg atgctgacgt ttccggtacc cgcgaagaga tcgagcagct tgatgccgga
    18661 gagatcgtgc ggcaggatgt taaatagggc ttccttcact cggttagatg ttgggcgaac
    18721 cgcttgtccc ttgggtactt tcaggcgccg ccctctggcg tttcctccta tgactcgcat
    18781 gttcgctcag cgtttagctg gcaattattc ttcgtaacct gcgactcaat gtacatgtgg
    18841 aaatgatgta tgacatgaga cgtcgatgtc ccgaagccta atttgaggat cactgtagat
    18901 cggaacagtg ttgggtttaa gcaggcgcca tagagcttta gaatagctct cgctccgata
    18961 agattggcga tagtccgcat tatcagggca aagctgatca accagccatg cgtctcgcgc
    19021 tgaacagctc tcacaaaccc cgataagctt cttcgatgtt gcaggtgata ttctggaagg
    19081 ccaagcacaa acgagttctg cagccgtgtg gtttagatga gatttgccgc caacgttcag
    19141 gtcacgcgga aaacgaagtg ccgcagccgc aggttccggt tgcgttgggg ttggcgaatt
    19201 taaagccgcc gccgtagagg cccgagacga aatctataac tgtgccttgg agaaagggga
    19261 tgctttcagg atcgatgtaa acagttagtg tttcgcaatc taagatgatg tcgcctggct
    19321 ttggttcttt ctcaaaattg agctggtagg agaagccgga acaacctcca tcagaaaccg
    19381 aaattctcaa tgcgtggtcg atatactcgc tacgagcaag caatgatttt acttgctcaa
    19441 cagctttccc agtcagcttg agatcaactg cgactttatc gatagtttcg gtcattttgt
    19501 cctcttccac tgagcttaaa gcgtaatggc gacctcgccg catgtcaagg cggtcaccca
    19561 ctcaggcaag aagcaatttt atctctcaaa cagcgcctcg acgaagcgcc cagcttcaaa
    19621 tgcctgtaaa tcgtcgatat cctcgccaac ccctatgtag cgcaccggca ctccaagctc
    19681 ctcctgaatg ctaatgatga cgccgccttt ggcagttcca tccagcttgg ttaaaacgat
    19741 accgtcgata tcagttgctt ctttgaagac cttggcctgc tgaagcccat tctggccggt
    19801 agtagcgtcg agcacaagaa gcgtttcgtg gggcgccccc ggctgctcgc gcgcgatcac
    19861 ccgccggatc tttttcaatt cctccaccag atgcaccttg gtatgaagac ggccggcggt
    19921 atcgatgagc agtacgtcga tacctctgct cttggcagcc tgcatgccat cgtagacaac
    19981 tgccgaagga tccgaaccgg tgcggtgctt aatcaactcc acaccggctc ggcggcccca
    20041 agcttcgagc tgctcgaccg ccgctgcccg aaatgtgtca cccgcaacaa ggagaacctt
    20101 cttgccgtcg gccttatatt gagcggcgag ctttccgatg gtggtggttt tgccgactcc
    20161 gttgacgccg agaaatagca cgatccaagg ccgtatggat aatggttgaa ccaacggcgc
    20221 tgcggcggag tcctgcagca gctcaaccat agctttctgc aattgtgatg acatgtcctg
    20281 cgccagatct tcgctgccgc tgggtgatag tcctcgcaat gtatccatca acttctgcgt
    20341 tgctttcacc cccacgtcgg cagtgagcag gctctcttcc atctcctcga gcgattgctc
    20401 atcccattcc cggttctgaa agatggatcc cattttttgg acccaggttt cgcggctctt
    20461 ggtaagcccg tcttttaatc gttgaaagaa agaacccgcc acgttttgat tccctgaaag
    20521 gcttgcgagg aaggaccgcg atggatgccg gcgctcgagg gtgtttgccg ctagctcatc
    20581 cttaccgaaa ccactttcga caccccgggt tctgccatgg tgatgccata gagaacctcg
    20641 gcggcttgca tggtcttttt gttatgcgtg atgagcacaa attgcgaaaa ctcgctcatt
    20701 tctttaatca gctcattgaa gcgatcaacg ttggcgtcgt cgagcggcgc gtcgacttcg
    20761 tctaggaaac aaaacggact tggtttggtt aagaaaatcg caaacagcaa actgaccgcc
    20821 gtcaatgcct tctcaccgcc ggaaaggagt gtgatggatt gcagtttttt ccccggcggt
    20881 tgaaccacga tgtccacgcc tgtttcaaga taatcatttt catcggtcaa caccagtctg
    20941 gctttgccgc cgcggaacat ccgtggaaaa atcgattgga acttctcgtt aatctcttcg
    21001 aagctctcct tgaaacgaac gcgacagaca cgattgagct tgacgattgt ctgctgcaga
    21061 tcggcgatcg atttttcgag gtcttccttt tgccggttca tgaactggaa gcgtgtcgtc
    21121 agttcttcgt attcgccaat ggcagccatg ttaacgtcgc ccatacgttc gatgcgtgcc
    21181 cgcagttcgt cgatttcgat cgtcagctcg tttgacgcag tcgcgataac attcggcgct
    21241 tccatggacg cgagatcgag gccatacttc tcgcgcacgc catcggcaag atgctgcaga
    21301 ttgagccgct tctcggtgag acctacctgg agccggttct tttcgtcctg agacgtttcc
    21361 acccgaggtc gcagctcctt tacagattca tcaatttccg tcagctgttt cgaaatttgc
    21421 cgataccgct ggcgctcgtt ctgcagcttc tcctccagct cttggacggt cgcgcgactc
    21481 gaagccaccg tttgctcggt ttcggtaagc gcattttgga tttcgcctct gcgccgatgg
    21541 atatcggcga tttgcccgcg gcgatcgccg atctgctggg cggcttcctc ctgaagcttg
    21601 agccggttat cgaggttcgc gtgagtgttt tcgcgtttct ctcctaaagc cgcagcgcga
    21661 attcgcgatt gggtcaccgc cgtctcgacg gattctaatg actgtttgcg ttccaggaat
    21721 ctggtctgga gtccggcaag cctctcctcc cgttccgcct tttcaagcgc gcgcttttcg
    21781 cgaccagcat tagcttctgt gatttcctgc atcagatttg ctacggtcgc gttcaaatcg
    21841 cgttgctcct ggagcagcac gtcgatggtt tgcctgagcc gttgtgagtc ttgctgagtc
    21901 gaacgatgtt cgtgctcgtt tcgaacccgc tccagttcca ggcgatggat ttcgcttcct
    21961 aatagcgtct ttttcgcttc ggcttgttgt aaattctttt taagttcaag gagctctcgt
    22021 tcttcgattt gcatgtcagc ttcgagttcg gtgagcaaac cccgaagctc tttcatacgc
    22081 cggcgctggg ttaagaagct gccttccagg gactcttcgc ttcctccggt gaccactccc
    22141 atagggtcga taacctctcc ttcgggggtt accagagtgc tgtaataacc attgcgatcc
    22201 cacaaggcca atccggatcg gaggtcttgc acgacaacga catccgatag gagatattcg
    22261 gcaacatctc gataaccttc tttgaccgaa attaactcca ataacggggc gaccacctcg
    22321 gcctctccca gtggtaacgg acgattactc ttgcgcgaca tctggaccgg gatgaagctc
    22381 cctcgcccag aggccttttg cttcaggaat tcgatggcct cgatgccttc gtcatgacct
    22441 ttaacaatca tatactgcag gcggtctcct aaaaccgccg tcagcgcttt ttcatagaga
    22501 accggagcct cgatcacctc ggtaaccaaa ccgtaaacgc catcggcggc agcttcctcc
    22561 tgccgcttta acatgatggc tcggacgcct tcttgataac cttcgtaatt cttttgtaga
    22621 tcttcgagag ataatagtcg ggagcggctt tcttgtatcc gttccttgag agcctcgatc
    22681 ctttcttcct gctcgtctct actccgggtt agaccttgga tcagggctac aacttgggca
    22741 aattcaagat ttcgctcttc ggattgctcc agagaagcct ctagagcgcg ttcggcttca
    22801 tttcttttgc cttcaatcag ctcccgagaa ctggccgcgg ccgagtgctc ccgctggctt
    22861 cgctccagag cgcgctccgt ttcatcgcgc cgccgttcct tatccgtcag agtatttttt
    22921 aagttgacaa gctgattggc gatgtcgatc agcgccgctt tctcggtctc aagcccgtac
    22981 tccagcgcgt gaatttcggc ctgaagtctt tccagctcgg tctcattttc gcgtagaaag
    23041 gtctcctcga aaagcgacag ctgaatgaag ctttctttgg ctttattgag ttcttcaatc
    23101 tctcgtgcga gagtccttag cttatcctcg agttgcgcca atgcgctcag gccttcagcc
    23161 tcattctcgt ctagttgcga caaatccttg ttaaataaat cgatgcgctg ctcttccgat
    23221 tgaagctgca tctttgcttg atagacattc tcctgctgca gactaatttc cttgtccagc
    23281 tccatgagtg aaaagcgcac ggcttcgctc tcggattctt taccgtgcaa attgttcaaa
    23341 tattcggcca ggcgattttc cgcatcagcg aggcggtttt catgggtggc gatttcctga
    23401 ttcagcgcct gacggtgcag gtttgcgcat aacagatctt tttctcggag ttccgctttg
    23461 aggatcttgt agcgctctgc cttctttgcc tgcaactcca ttgtgcggat ctgtcgctca
    23521 atctcccgaa cgatatcggt aacccgaaac aggttctgct gcgtgcgctc gagcttgcgc
    23581 tcggctacca gcttgcggct cttgtattta ctggtgccgg ctgcttcttc gatcagaact
    23641 ctacgctctt caggttttga gttgacgagt tcatcgaccc ggccttgctc gacgatcgaa
    23701 tatgccttgc tgccgacgcc gctgcccaaa aacaattcga tcacgtcttt gagacggcag
    23761 ggggtcttgt tgatggaata ttcggattcg ccggaacgga agagccgtcg ggtcaccatg
    23821 atttcgctga agctactgta ttcgacaggg ccgcgcccgt cttcattgtc gaaggtcagc
    23881 gaaacttccg ccattcccgt ggcgggaagg ctgtcactgc ctgcaaacac gacatcttcc
    23941 atttgatgac cgcgcaggtg acgcgcgctt tgttcgccca tggcccaacg taaggcgtcg
    24001 actatattcg attttccaca gccattcgga cccacaatcg cggtgattcc cgggtttagt
    24061 tcgaccaatg ttttatcggc gaacgactta aaacccacca tctcgaggcg ttttaggcgc
    24121 ataatcgttt aatgtcggac acttacgaag cgacggagtg aatcaatgga ttgcttggca
    24181 atatagtcca tcggtcggat aattgtcaag aaaattaacc atatgaagtg gctaaaggcg
    24241 tcatttacta ctatatatag gaagggtgga ttatttttcg agcagttcgg tggtccagta
    24301 ggacgcatcg acggtgttca ggaaagggag ccattcctta taacgagaga gactgaaggt
    24361 ctcctgcatg aaaggggtcc actgaggtct aaccggccgt cgcgccagtg tcataccggc
    24421 ctgttgcggg gtacggcctc cctttagtcg attacattct tggcaagaac acacgacatt
    24481 ttcccaggtg gatgtgcccc cttgcgtcct gggtatcaca tgatccaggt tgagttcgtg
    24541 ccgcggccgt ttctgaccgc aatactggca agtgcactta tcgcgagcat agatgttata
    24601 ccgactaaag cgaacctgac gcttgggcat ccgatcatag ctgacgagca gaatgaccct
    24661 cggaacccgg attacccgat tgaccatgcc gaccgattcg tcatggaccg tcaccgagag
    24721 atcgctccaa ctgtcaaaat cgaaagtttg gtactgctca ttcaccgctt tcgcaatccc
    24781 ctgataaagc agagtgaagg cgcggcgcac cgaagtgaca tggatcggta aataggaacg
    24841 gttgagaatc agtaccttgg tgttgagtac ggaatagacc gggatagtag cggaatagtt
    24901 cattcaagct tggcggacct cgaggatcag cccgccatta gagctaaaat acggggccgg
    24961 tcacacaatg tcaaacgaat gcataatgtt actatgctgc ttgaagtcgc acttgaatta
    25021 ctctataaag acaccagccg ccatcgatga ctgggacttc cgatgccgaa cggcattccg
    25081 ttcccgcatt ccccgcctct tccgagcatc acgccgaatt ctatagggta tctatgaagc
    25141 aagaaatttt tatcaactcg tctcctcagg agagccgcat cgcaataatg gaagacggtg
    25201 ctctcgccga gtttttgatt gaacgcaaag aagagcgagg cattgcaggc aatatctaca
    25261 aagggaaggt cgaacgggta ttacccggta tgcaggccgc gttcgtcgat atcggcatgg
    25321 acaaagcggc tttcttacat gcatcggatt tttttagcgt tcccgaagat gtccaattga
    25381 tctcgccctc gggagacgat gtcgaatttg acgacgttcc taagccggcg cctagccgcc
    25441 gcttgccgct tgagaagcag gtttcacgag gcgaagaaat tctagtccaa gttgccaaag
    25501 atcccttggg cacaaaggga gcccggatca catcgcatgt atcgctccca ggccgttaca
    25561 tggtttttat gccgagcacc aagcatatcg gcatctccag gcgaatcgag agcgaggaag
    25621 agcgcaagcg ccttaaagag attgctcaat cgttgggcac gaccgaaggc ggtttcattc
    25681 ttcgcaccgc gtcagaagga caaagcaaac gggaaatcca acgagatctc aggtttctta
    25741 ccaagctttg gaaacgaatt caggagaaag ctgaaaaggc tccggcgcca tcgttgattc
    25801 accaggatct cgacttaatc acgcgcatga tccgggattt ttttaccacc gataccgagc
    25861 aggtggtcat cgattcggcc aaagaccacc ggcgcatcgc agattttgtc ggccatttta
    25921 tgccccggct caaatcgaag ctcgtgttat attctgaaaa agagccgctt ttcgatcgcc
    25981 acggtatcga agagaagatc gagaaagctc tggaccgccg cgtctggctg cgttcgggcg
    26041 gttacatcat catcgagcgc accgaggcgc ttaccgctat cgacgtcaat accggacgct
    26101 tcgttggcaa aagagatcaa gaagaaacca ttcttaagac caacatggaa gccacttcgg
    26161 aggtggtgcg ccaacttagg ctaagaaacc tcgggggtat catcatcatc gattttatcg
    26221 acatggagaa agaaggaaac cgtaagaaag tctatgaagc gctcaagaac gcgctcaaga
    26281 aagataaagc ccgaaccaac atcctgaaaa tttcggagct cggtttggtg gagatgaccc
    26341 ggcagagaac ccgagagagt ctggagaacc aactgctgag cccgtgccct cattgtgatg
    26401 gccggggccg catcaagtca tcagtgacga tcgcctacga cattttgcgg gcaatcaaga
    26461 agcagcaggc gagcctggaa aatggcaaga acatcgtgct gcggcttcat ccagcggttg
    26521 ccaattttct ttacgatgag aaggcgaaca gcctggaggc gctggagcgg gaaatcaatc
    26581 acaaaatcat catcaaagtg agcgaagact tacgccacca gcaatacgaa atctccacaa
    26641 cctgagtcta gctcttgggc caaggtcgga tttctgattc cttccttaat ttgctcgaat
    26701 cgtaaacttc cagatgagtg aatgcggtgg gtttgcgcct aggttttgcg gcgggccaac
    26761 tgcgcgcgtg ccctatgggt acgacccaca gaagccgcag gctggttgga actttgaaca
    26821 actctctgag ttcgggctca acttcctcac gcacggaaac ccaaacagtg cctaaattca
    26881 tactcgcggc ggcgagcatc atctgctgca ccgcgttagc gatgctggcc tggaagagct
    26941 tctcgcggtc tcccggctgc cgagtcgtca ggtaaacctg cttggttctg gcatcgccgc
    27001 agaccagaac gatgacactg acgtcaccca cgtaatcttt cttaaggcct ttgtagttga
    27061 tcggatcttc gcgttttctc tgtcgccact cgttatcata aatctgccgg atgcgtctca
    27121 ttttctcgcg gtcccgagtc acgactagtt cccacggctg ggtgttggcg ccggaaggcg
    27181 cccaacgagc cgcttccagg atcagttcga gctgctggtc ggtaactttg ccttttttgt
    27241 aaacgcgaac gctgcgccgt gtctgaatga cttcaagtaa gttctgcgga tccatgcgtg
    27301 atagctctaa attgccctcg aaagaattca tcgcacaaaa agcctaaagt aaaaaggaga
    27361 aagctggtca aaaaatgatg gtccagcgtt ccactttgat acttcagctt actgcgtctt
    27421 aatgcgctga aatctgactg cgccggccgt accaccatgc cgccaaaatc agcggggcaa
    27481 gaacgaccgt catgagcaag gtgaccgccg acgccttatt gatgctgccg ctgtaccagt
    27541 agctccagat gactgcgggc agggtgatgt tgtcatgcac cgcgagaaaa accgccacgg
    27601 tcaactcgcg atacaccaac agcgcggacc aaatccaaac ggaaaaaagg gtagggcgaa
    27661 tcaacggaaa tagcacgcgc caggcggttc ggatggtcga aagacctgcc acgaaagcgg
    27721 cttcttcgag ctcccggtga atctgtaaga gagagccgtt aagcgcgcga gtggcaaagg
    27781 cgaggcgggc aacgacgtaa accaccgcga tcagccagac cgagccgtag agcggaatcc
    27841 tggtgccgaa aacaaacagg gacaggagca gcgcgccgat tgcgaggatt acctcaggca
    27901 gagcatgagg cagaaacgcg ccgaactcga gtgcataacg ggcccggctg cgcgagcgca
    27961 cgatcagcca ggagatacta aaggcgagca atagcacggc tagcggaacc actgccacta
    28021 gtatggccgt gttggccaag ccgcgcatca ccagttccca atccatgccg taaaaatggc
    28081 tgaatgaaag tttacccagc atgtctatcg acggcgggac gaaatagggt gtcaaagcgg
    28141 cataggtgat tagcagcagc ggcagaagct tggacatcaa tgtgtaaccg gcaataaaac
    28201 accaggctgc gatggaccac ttgcccagag gcaggagtgc gggtttgtac ccctttccag
    28261 tgacgacttc aaaacgatga ccttgccgca ggacctgact gtaccagaga gtaagcaagc
    28321 cggcgacgat gatcatgaac gcgccgacaa ctccggtgat gccgtactca ggcaagccgg
    28381 cgccctgcgg gtgaactttc aagtagataa atgtgctgag catgtagacg cgattgccca
    28441 aaccaagtat cgccgggatg tcgaatgtag caatgccgat tgtcaggata tagatgaccg
    28501 ctgccagaat gccgggacgc gccaacggga gggtcacgcg ccaaagaatc ttgccgtaac
    28561 ccatgccgtg cacgcgcgcc gcttcctcga gacttggatt catcgcgcga aacatctgcg
    28621 cggtcaggat gaacgccaag gccgagagac tgagtccttg cacgaatccc atcccgatag
    28681 gtgtggcaat attgatcggc gcatcatcca ggttgaagag atcaacgagc cagcggttta
    28741 aaattccgat gcgcggatgg gcaatgagtg tccatcccat cgccgtgtag atgcccggaa
    28801 tcagcagccc cagtgtcata atcgcataga tcagcgtctt gcctcgaaga tcgctccgtt
    28861 ccgccagcca agcgatcggc aatccgaaaa tcagcgcgac catagttgcg gtggctgtga
    28921 aaatgatcgt attggcggcg acccgcagca caaaaggatc gctgaaaacc tccagataat
    28981 tggcgagggt gtagatggca cctggcgtgc cgacggcgcc tttctgaacg ctaacccaga
    29041 acatcaccaa caccagcgcc aggatcgcgg ccatcggcac aagcgccacg gacaagaggg
    29101 ccgaagaagt ctggcctctt tgatcaaggc gtgcggcgaa tcgtagtaag cgtcgtttca
    29161 tatggattga tctggaacga agctctttta ttcgaaaatg attccggtta catccagata
    29221 tcattgaggg cgagcgagga aggatctgac cctagccggc caggcataac ttcgcccttg
    29281 gctgaagacg acaggtgaaa cctccctctc gctgacatag tacgaatcga tttacaccga
    29341 tctgattgtc gcttttggtg acattttgct gatctatttg ccggcatcgc gtagaatcgc
    29401 ttcaagctcc ttctgcgttt cagcatagtt tttgaacgat gcgagccatt caggtgagct
    29461 gatgatgacc ttgcccttgg cggcgcggac tttgtcgagc tcggttttgg tttgcgagcc
    29521 gggaaataga tgcaggtcca aaccatcgtg cttccataat aattcctgcc cttctgcgct
    29581 cattaagtaa ttgatcagaa gcgcgccggc attaggcgag cgggaatttt tcggcacagc
    29641 cgcgtaccgc atgtgcagta cggtgccctc ctctatgacc gtgtgaccca atggcgcgcc
    29701 ggcgcgcttc aagacgttga cgtcattgcc gccgcaggta aaggccagca tgaggaattc
    29761 accgctggta atccgctctg cctcgccgca acgcatcaat ccgccgatct gcttggataa
    29821 ttttttggtg aagtcgatca ttttctcgcg ccccagaaaa tcgggcgtgg caaattcccg
    29881 cattccggca gcatagggag tcgaagcaat cttgcctttc cacttcggat taagcacgtc
    29941 ttccaggcgc cggggaatgt cggctccctt gacgagattg gtattgtact ggatgccgac
    30001 caccgcggtg gcgacggcga catgggtgcc atctgccgca atcgcgttaa attttccctc
    30061 ggattttggc ttctcttcga tgaaagcgtt gtacgtcatg ggccttagca ggttcgcctt
    30121 taacccatcg aacattgcct gagcgttgcc cagatacacg tcggtgctgg cgggttggcc
    30181 agcggcgttt tcctggataa tgcgcagcat caaacgctgc atatccggcc cgggcgtgta
    30241 ctggcctttg agatcgagct tgtatttttt gttcatgcca gcgagaattt ctttaaatcc
    30301 ctcgctcccg ccaaagctgt tttggcccca ctgaccgcgc aacatgccct ctttcttcgc
    30361 gccctcgatg atctgggtca gagtctcggc ctcggcgagc gcagccatgg tggctaacgt
    30421 gaccatcacc gttagagccc aacttgctag ccgcggcgtt gattggtgcg cgatcataga
    30481 gcctccttcg tttacattga tcacaattca aatccctttg aattacatcc gggccggaaa
    30541 caccatgatg tcggacgaac tcacatgaca taatgccgtg ccgtcggagc tttccggaac
    30601 tccgcggctt tttccgaaca gctgccggtc gttgaccacc gccgtgtaga caaattgatc
    30661 gccgagaaaa gtactcgagc cgatcttggc gcggaaggag ttgccgtcga agtgcgcgcc
    30721 gggatcgagc aatgaaagac actcgggacg aaaaccgagc aatactttgt cgccgatctt
    30781 cgatgaactc tcgatcctca atctgccgat ggctgaatcg gctatgccgg gttcgatcat
    30841 gtgaccgtcc aaccagttaa ccgaaccgaa gaactcggca acatcggcgc agttaggctt
    30901 gtgataaatt tccaagggcg cgcccacctg cagaagttcg ccggcttgca taagagcaat
    30961 cctgtcggcg attgccatag cctcgatttg gtcgtgggtg acatagagca ttgtcgagcc
    31021 gagctgcttt gccaacgcgc ggattttgcc gcgaacttct tcccgcaggc gggcatccaa
    31081 attactcagg ggctcgtcca tcaagacgat ctgcgaattc accgcgatcg cgcgcgccaa
    31141 tgcaactcgc tgttgctgac cgccgctgag cagcgtcgca ggtcgatccg cttgttcttc
    31201 cagttgcacc agccgtaaag cttccttgac gcgtttggcc acctccgggc gaggaatccg
    31261 ttgcgatccc tcagccagcg gcagcgcgat gttctcgcaa actgaaaggt gaggccagac
    31321 tgcgtaggac tggaaaacca tgcctagttt gcgttgctgg ggaggtaccc aggttggagg
    31381 attgtcagag gagacgactc tatcgccgat gcggatctct ccggaatcag gagtttcgag
    31441 gccggcaagg cagcgcagca gagtggtttt gccggagccg ctggctccga caatgacaaa
    31501 aagttcgcct tcggctattt cgagatcgag ggagcggatg gctgcgatga caccgtcgct
    31561 aacgtcgaaa cgtttgtgca aacggcggat tgagatcata gaatgacgtt agctaatttg
    31621 ctgggattgt ctcagcaaca atggtacgtc gtgcgcttgt cgtgccatgc ggagatgatc
    31681 cggcacagat tcgtggaaat acctccgaga gaaatagcgc ctggacggcc gaagcaaatc
    31741 cggccagacg agacaatctg ctgacagtga atcaatgata ttcggcgcct ggtaatggat
    31801 tggcaaccgc ttcttccagg gtagtggcgc ggcgccactc gcttgccgga cggctttttc
    31861 cgtcttctcc aatctgatcc atggaagcgt aaatctcgat ttgataaccg tccgggtcaa
    31921 gaaattcgac cccaggattt ccgcccggcc cgcggcggcc ttcatacatg atggtcactc
    31981 ccttggcctt caggaaatcc cgaatcttga agagctccga aacggtggcg acctcgaggg
    32041 caaaatgatc gaagcccact tggccccgtt tcggcagcgg cgtgttttcc ttggcttgca
    32101 tcaatgcaac cgtatgatga tcgctcgcat tgcggaagaa taccatgccg gcttcattgc
    32161 gatcggagaa tttgaagccc atgatctcag tccagaattt actggtccgc tccaaatcac
    32221 tgaccgtgta aaccacatga ccgatttttt taatcttgat tggagactgt tcgtccgcca
    32281 taaatcctcc ctgttctgtc tagcatgctg ctgaaaactc tgattgcagg ctgctcaaaa
    32341 agatccagag gcgaggcgca caaaaatcga cgagcagaag cgtactcaca gtacgttgga
    32401 gcaacgcgct tgagcgcaac gaagcctatg ggctttttca gcaacctgtt aactgtgcgt
    32461 tgtctatcac ggttgaacat tcaaggcaaa accaaaagcg gcgccgcagg cgggcgcttt
    32521 aactatttag tgcgtagctt gtcgaccaat tttcgactgt aatctctcac tgagcgcaac
    32581 ggcatggagc gccgcttgag catgtccaag cggattagct tgaactggat actcttggcc
    32641 agggcgcgcc gctcgccttc gtcctctaca tgcttcaaaa gatcttcgat ggtatggatg
    32701 tcttttagca gctccgcttc aggcggcagc acgtgggcgt tcttgagaac gagatagccg
    32761 atacgcaaat cctcgggaac ccagctcaga tcctccagat ccagcggttt gcccttgcct
    32821 ggcagattgt cgaaggcgcc cgcccgttga gcttccttta ttcgctcttc ggcgatgcgc
    32881 cagaagtgac tcatgcgggg ctcctttcaa cggtggcttc cgtgatctcc tccggcggct
    32941 tgatgtggcg aaacatcaga taaaaggcga cgtcgttgat caattgtaac ccgccgccga
    33001 taaacaacgg aagagtcagg gatacgcttt gcatcagata ggtcgaaatc gtcggactga
    33061 tgccgaaggg aacgatgcgc gccaagctgg tgactccggc ggccgaaccc ctctcactcg
    33121 accgcaccat cgccatagta aaggaggaac gaagcgggtt gtccatgtaa gcaaaataag
    33181 agcggacagc gagcatggtc gccgcgatct taaacgtcgg cgccagcggg atcagaagat
    33241 agatcaccga tgccagccca tgggaaaacg ccatcgtttt aaccagcccg atatagcgcg
    33301 cgatgatcgg cgcgctcaga aaagataagg ccgccagaaa gtaggaaatg aaaaacaaca
    33361 gtccgaggga ttttagctcg acgccgtaac ggaggaaaaa ccagtaggac accaatgatc
    33421 cggccaggcc agcgccgaaa ttatcgacga acgacaagag cgacagttta gttacgaaaa
    33481 caccggtcgc cttggaaatt ttttttcttt cggtcttgcg cggctgatgt tcttcacgga
    33541 tctggctgta aacataaaac aggacaacgc tgaagatgat cgtcagcatg aacagcggtt
    33601 tataggagga cacggcagtc cagccgcgga tttgctgcag gtactgcggc aaaccggccc
    33661 cgagcgcgcc cagggaaccc ataatcgagc cgacgaaaga gttcagcgcg aacacttggt
    33721 tcctgttttc cggtttgcac ttctcggcga gcaatgcttc ctcgaccgga ttgaaagggc
    33781 cgccgccgga accgccgccc ccggcaccgc cggcgccgac attggcgatc accacggcta
    33841 ggagaatgaa aaccaatcca ctgaagaaaa tgaaaatgct gttggacacg atcgtcagga
    33901 ccgctaggag catcaaaatc ctgcgccgcc cataacgatc cgacaggtag ccccatacgg
    33961 cagtcagcac cgcgccggac aagtaggcga cggaaaagat gatgccgatc atgaccggcg
    34021 tatagccgag ctggtccaga tagatcgcga agacgacatt caaataagaa aaagcgaagc
    34081 ttcgaatccc gcgcgaggcc atgagcagct tggcgtcgtg atgcaattct ttaaacatca
    34141 gtcggttaag ctttctgagc ctgatagcat tcgcgctcgg catccaactc ggtcttgatg
    34201 ccgattgcgt gcaaagcgtc gtttgcttga tcgagagagg agctaccgga gacggcttgg
    34261 gcgggacggg aaagggtttc aaacactttg gttgccgaca tgaaaaaggt caaggcggtc
    34321 aagggtgtga gcttcacgcg tcggccgccg tcacgaattt ccctttctcc agaaacgccg
    34381 cggaagcctt ggaccggaat tgcgctcgct tcggtaggaa ctgtcttgat cactgcttcg
    34441 agctcggctg cggttttggg agtcagtgac cacgcgccca acaaaccacc ggccccggcc
    34501 agcgcggtga gagcggcttc aatttcgtcg atggttaatt caccgtcgct gccatagccg
    34561 aaaactcccc aaagcgcgcg atagccgcgt ttctcgagct cgacgaacgc cgccagcatc
    34621 atggaatccg ctaacgggct gcgcaggcct ggttcagtgc cttttgccag ggagtcgccg
    34681 ccgacatcga ggccgacgag caggtcggtt tgcaatactt cgatggcgcg ctccaatccc
    34741 gccacgactc ctcgcactcc gccattgata tcgacaagca agacctcttg cgctaaaaat
    34801 tccgccattc tcgattcggc gaagtaaacg gcggccttgc tctgcgtttc gcgattagcc
    34861 atccaggcgt actgatgcag agcgcgaacg ttgtcgacct cgctcagcat gcgcggcccg
    34921 ggcagcgggt cgaaaacccg gcgttcccag gaaagaccgc cgaggacaaa ctccaagccg
    34981 caaaattcga gaaatctggc agtcgccagt gcgccggcga catcgccgcc accgccgatg
    35041 ccgattacca ggctgcgcga agattcgcct attatatcct cgagagaagg agagccggaa
    35101 ctcatttagg aagg
//
LOCUS       DASUVS010000103         7175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_470960, whole genome shotgun sequence.
ACCESSION   DASUVS010000103 DASUVS010000000
VERSION     DASUVS010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7175
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470960"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VF182_05820"
     CDS             <1..241
                     /locus_tag="VF182_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HEX6436628.1"
                     /translation="HTQGACGAIETVAAVMSIHKKTILQSINCDALDPGCLIRVHREK
                     RTQGNIRALLLNTFGFGGKNATLILCPFGTDTRAI"
     gene            251..2440
                     /locus_tag="VF182_05825"
     CDS             251..2440
                     /locus_tag="VF182_05825"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6436629.1"
                     /translation="MFGLTDAAHIGISAALTSMTTAALGSFVLLKDPKSSLYRVFSAY
                     SFSITVWSTLLAFHVFAENRSVAEYTARYLHVGATLIPTLFVHFVLQFFYSRKRKTIN
                     LLVAPLYVITATLMFLCINGTLVSEVAPKYGIKYLMVANPGYIYLVLYFIACAAWGLS
                     TLLVGYLKAEGVRKNQIKYLLFGSLLGYFGGLDNFLYLYDVTIFPLFPYGSYAIPIYV
                     SSTAYAIAQYRLLDISVAIRKSIIYASLLFMLLVPCYILVIWGQQVAFGSINYTFSMA
                     TLLLFIIVGFLFPKIRFRTEEALERALFQKRVNYRETLLYSSKDMVSIVDLESLSNNL
                     VRTIGRALGIEKASLFLLEEAKGVFSLKASIGVAIDPQLFLLRDDPLLVMLTKAQNGI
                     VREELEMDSENLNWRRTSRRMEEIEAEISIPIMSKEKLIGALNLGYKPGKEIYSNEDL
                     ELLSTLANQAAVAIENARLYQNLKQSQDTLRRADRLSSLGLLTAGLAHEIRNPLVAIR
                     TFTQLLPERYEDAEFRNGFQSLALKEVDRICGLITDLLSFARPSKPNVAQEDMNNVIE
                     GIARILDTQAKEKSVELVRHFAPNLPKVWIDREQMKQVFMNLIFNGIQAMNDGGTITI
                     STRIFTSDAIEQAPSFVQVEVRDTGVGIPNENLEHIFDPFFTSKDEGSGLGLSISHQI
                     VQEHGGYVTVESKVGKGTSFLVNIPIGKPVRAMTERNLHVHEANLSH"
     gene            2418..4295
                     /locus_tag="VF182_05830"
     CDS             2418..4295
                     /locus_tag="VF182_05830"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6436630.1"
                     /translation="MKRILVIDESEVIRETLALILGRDFSVVKKFFGSSGFSLADTGA
                     DVDLLIIGVSPALRSEAGKLLRFAAQVSCAVLFLVESRSAIKLIEEQETIGCLAKPFN
                     PYELKQKVGTLLARTAVLPSVPRLRAISNNPPHSHYLRFPFLDRTAAALVQRFARTQL
                     PLLVSGEIGCGQERVLRGICALVGADTACTFVNAADVNDEFLASKIAQLSWRAEKSGR
                     IFVVEEIEKLSSSAQSKLLNFIEQVETTSADCRFLATSKIDLLEQVYQGIFLEGLYYR
                     IATLSLKLRPLRDRRNDIPTIAAWFAEYFAPLLELSQVSFSPEARDRLANYLWFGNLS
                     EMETVIARTLAIRRKTTIEAHDLVFDFFGTVEAGDLAEGAEFSSPEEKRGNLRVVSSW
                     TADTQVPSPQVGQTPCSERASELKVLIHELAHEMKNPMVTIKTFAQLLDDRYEDQDFR
                     ARFRDVVSGDIERMDDLLEIMIEFADFNQPRVDRTSVDERLGWAVGELREECGKRQVV
                     IRVQSRSISTEILADENQLDYILKNVLSAVVSQAKVCTEIGVEIQETGTIVVSYLREG
                     PRMASITQYLGSLSEAAGESILPLRILLAKQLVERNGGEIAVDGTDAEKEIVTMEFPI
                     A"
     gene            4288..5718
                     /locus_tag="VF182_05835"
     CDS             4288..5718
                     /locus_tag="VF182_05835"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6436631.1"
                     /translation="MLNREKKSDMTQGVLLVIDDEPGVRHSLKMVFDKVFRVVEAATA
                     NDGVQKVTEEKPDIVLLDIVMPGADGLSVLKQMKSEHPECQVIMLTGLNTARTAFTAK
                     STGAFDYVTKPFDVEELRLRVEHAFEKVQLSKELERLKEEVGRKYGIDHIVGKSKQII
                     DIFKAVSMVAAKKSTVLIQGESGTGKELIARAIHYNSDRRSKPFIVVNCAALPDTLIE
                     SELFGYEKGAFTNASQKKIGRFELAHGGTLFLDEIGELNLSTQAKFLRAIEQETFTRL
                     GGTDDIKVDVRVIAASNRDLEILAKAGEFRPDLFYRLNVVSLYLPALRERREDIPLLL
                     DHFLRLKAQEHSIAVKSLSPEVVDFFTAYHWPGNIRELENLIERLTILTPHDQIMLRD
                     LPVGMRSTDHTATLKEDVLSGSRPLSEAVDEFERELIVKALQRTGFNQTKAALLLGTS
                     RRILKYRIEKLKINESHSGDEPKLST"
     gene            5848..6969
                     /locus_tag="VF182_05840"
     CDS             5848..6969
                     /locus_tag="VF182_05840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6436632.1"
                     /translation="MDSNKPHILVVDDEMGPRESLKMILNPYYTVHVAERGGQAVDFL
                     NRFPVDLVTLDLKMPGFTGINVLEKVKQHDPDIEAIIITGYGSLDTAIEGLRLGAFDY
                     ISKPFDVNHILSLVRRGIERRGAKTKLRQVRSDFLSNVSHELRTPLSVVVGFVYLLLN
                     QVIGKLSEEQQKVLETVYRNSEELLELIDNVLWMTSLSSGDSSPIIEKFDAREIVDET
                     VKRYQRTIGEKNLSLTIDIPEGGASIISDRSKVERIFQNIFNNAVKFTSVGEIRVKAR
                     VSADRGVLDLQVADTGIGIEKSKMDSVFEPFQQVDNSIHRSFSGLGLGLTVARRMAEL
                     IGGTIQVKSEPGLGTTVLMRFPSKAAAPAAVPLEQRNYA"
BASE COUNT         1795 a         1708 c         1851 g         1821 t
ORIGIN      
        1 tcatactcag ggcgcctgcg gtgccataga aacagttgcc gcggtgatgt cgattcacaa
       61 gaaaactatt ctacaaagta tcaattgtga tgcgctagat ccggggtgtc tcattcgagt
      121 ccatagagaa aaacgtactc aaggaaatat cagggctctg ctgttaaata cttttggttt
      181 cggcggaaaa aacgcgactt taatcttgtg tcccttcggc accgacaccc gagcgatcta
      241 agcatccgcg atgttcggct taactgacgc tgctcatata ggaatttctg cagccctaac
      301 ttcgatgacg acggctgccc tgggttcgtt tgttcttttg aaggatccca agtccagcct
      361 gtaccgagtc ttttctgctt attccttcag tatcaccgtc tggagtacct tgttggcgtt
      421 tcatgtattt gctgagaatc gatccgtagc cgaatatacc gctcgctacc tccatgtcgg
      481 tgctacgcta ataccgacct tgttcgtcca cttcgttctc caattctttt atagccgtaa
      541 gcggaaaacc ataaatcttc tcgtggcccc gctgtacgtg attacggcga ctttgatgtt
      601 tctgtgtatc aatggaacgc tagtttcaga agtcgctccg aaatacggaa tcaagtattt
      661 gatggtagcc aatccgggct acatatatct agtcctttac ttcatagcct gcgccgcttg
      721 gggtctttct actctgttgg tgggatacct aaaagccgaa ggcgttcgga agaaccaaat
      781 aaaatatctt ttgtttggct cactgcttgg atattttggt ggcctcgata attttcttta
      841 tctctacgac gtcactatct ttccgctttt tccatacgga agttatgcga tcccgatcta
      901 tgtttccagc actgcttatg ctatcgcgca ataccgtctc ctcgacatca gtgttgcgat
      961 cagaaaaagc atcatctatg cgtcactttt atttatgctg cttgttccgt gttatatctt
     1021 agtcatttgg ggtcagcagg tggcatttgg aagcattaat tataccttct cgatggcgac
     1081 actcctcttg tttatcattg ttgggtttct atttcctaaa atacgatttc ggacggagga
     1141 agctctagaa cgagctttgt tccaaaagcg ggtaaactat cgggaaactc tgctgtactc
     1201 gagtaaagat atggtttcga ttgtggacct ggagagttta tccaacaacc tggtccgaac
     1261 gatcgggcgg gcacttggta ttgagaaggc ttctcttttt ttactcgagg aagccaaagg
     1321 cgtgttcagt cttaaagcca gcatcggtgt tgcaatagat ccccaattat ttctattgcg
     1381 cgacgatcct ctactcgtga tgctcacgaa agcacaaaat ggaatcgtta gagaagaact
     1441 tgaaatggat agcgaaaacc ttaactggag gcggacgtcg aggagaatgg aggagattga
     1501 ggccgagatt agcatcccga tcatgtcaaa ggaaaaactg atcggcgctt tgaatctcgg
     1561 ttacaaaccc ggcaaagaaa tttattcgaa cgaagatctc gagcttcttt ctactctcgc
     1621 caaccaggcg gcggtcgcca tcgaaaacgc gcgactctac caaaatctga agcagtccca
     1681 ggatacgctt cggcgtgccg atcgcctgtc atccttgggc ttactcacgg ccgggttggc
     1741 tcatgagatt cgtaatcctt tggtggcgat tcgcacgttt acccagcttt tgccggaacg
     1801 gtacgaagat gctgagtttc gtaacggttt ccaaagtctc gctctcaagg aagtggaccg
     1861 catctgtggg ttgatcacgg acctattgag ctttgcccga ccctcgaagc cgaatgttgc
     1921 tcaagaggac atgaataacg taatcgaggg tattgcgcga attttagaca cacaagcaaa
     1981 ggaaaaaagt gtagaattgg tgcgacactt cgcccccaat ctgcccaagg tgtggattga
     2041 cagggaacag atgaagcagg tattcatgaa cctcatattt aacggcatcc aggcgatgaa
     2101 cgacggcggt acaattacaa tctctacccg tatcttcact tcggacgcca ttgagcaggc
     2161 accctctttt gtacaagttg aagttaggga taccggcgtc ggtatcccta acgaaaatct
     2221 cgagcacata ttcgatccgt tttttaccag caaggacgaa ggcagcggct tagggctttc
     2281 tatctcccat caaattgtac aagagcatgg cggatatgta actgtcgaaa gtaaagtagg
     2341 gaagggaact tcgttccttg tcaacattcc aattggaaag cccgttcgag caatgaccga
     2401 gcgcaacttg cacgttcatg aagcgaatct tagtcattga tgagtcggag gtaattcggg
     2461 aaacgcttgc cctaattctg gggcgggact tttctgtcgt gaaaaagttt tttgggtcgt
     2521 cgggattttc gctcgcggat accggtgcgg atgtcgatct tctgatcatt ggcgtaagcc
     2581 cggcgcttcg ctcggaggcc ggaaagcttt taaggtttgc cgcgcaggtg agctgcgccg
     2641 ttctgttcct ggtcgagtcg agatccgcta tcaagctcat cgaagagcaa gaaaccatcg
     2701 gttgtctcgc aaagccattt aatccctatg agcttaagca aaaggttgga acgttattgg
     2761 ctcggacagc tgttctcccc agcgtgcccc gcctgcgggc aatatctaac aatccgccac
     2821 actctcacta cttaaggttt ccgtttttgg accggacggc agctgctctt gtgcagcgat
     2881 ttgccagaac tcagttgccg ctattggttt caggcgagat cggctgcggt caggagcgcg
     2941 ttctacgggg tatttgcgcc cttgtcggtg ctgataccgc ttgcacattt gtgaatgcgg
     3001 ccgatgtcaa tgacgaattt ttggcgagca agattgcgca gctaagctgg cgggccgaaa
     3061 aatcggggcg aatctttgtc gtggaagaaa tcgaaaagct ttctagctcg gcacagtcca
     3121 agttgctcaa ctttatcgaa caggtcgaaa caacatcagc tgactgccga ttccttgcga
     3181 cttcaaagat cgatcttctt gagcaagttt atcaaggtat attcctcgaa ggattgtatt
     3241 accggatagc gactttgagt ctgaaactac ggccgctgag agaccggcgc aatgacatcc
     3301 cgacgattgc cgcttggttt gcggagtact ttgcaccgct attggagtta agccaggtaa
     3361 gcttttcgcc cgaagcgaga gatcgcctcg ccaattatct ctggttcggc aatctaagcg
     3421 aaatggaaac ggttatcgct cgaaccctag cgattcgacg caaaaccact attgaggcac
     3481 acgatttggt gttcgatttc ttcgggactg ttgaagcggg cgacttggca gagggagcgg
     3541 agttcagttc gcccgaagag aagcggggta atcttagagt ggtctcatcc tggaccgccg
     3601 atacacaagt ccccagcccg caggtaggcc aaaccccctg cagcgagcga gcgagcgagc
     3661 tcaaggttct gattcacgag ttggcgcacg agatgaaaaa cccgatggtg accatcaaga
     3721 ccttcgcgca attactcgac gaccgctatg aagatcagga ctttcgcgcg cgatttcgcg
     3781 acgtggtcag cggcgatatc gagcgaatgg atgatctgct tgaaatcatg atcgaatttg
     3841 ccgacttcaa tcaacctcgt gtggatcgta cgtcggttga cgagcgtttg ggctgggctg
     3901 tcggtgagct ccgagaggag tgcgggaagc gccaggttgt tatccgagtg caaagcagaa
     3961 gtatcagcac ggaaatcttg gccgatgaga accagctcga ctacattctt aagaatgttc
     4021 tgtctgcagt agtgtcgcag gccaaagttt gcactgagat cggtgtggag atccaagaaa
     4081 cgggcacaat cgtagtatcc tatctccgtg aaggacctcg gatggcatcg attactcaat
     4141 atctgggttc tctgagtgaa gctgcggggg aaagtatttt gcccctgaga attttattgg
     4201 caaagcaatt ggtggaacgt aatggtggtg agattgctgt tgatggcacg gatgctgaga
     4261 aagagatcgt gacaatggag tttcccattg cttaaccggg aaaagaaaag cgacatgact
     4321 caaggcgtat tattggttat agacgatgaa ccgggggtcc ggcattcact gaagatggtg
     4381 ttcgacaaag tctttcgcgt cgttgaagca gctaccgcaa atgatggcgt ccaaaaggtg
     4441 actgaagaaa agccggacat cgtattgctg gacatcgtca tgcctggtgc tgacgggcta
     4501 tcggttttga aacagatgaa atcagagcat cccgagtgtc aggtaataat gttgaccggc
     4561 ctcaatactg cgcggaccgc cttcacggcg aagagcactg gggccttcga ttatgtgacc
     4621 aagcctttcg atgtcgaaga attgcggctg cgagtcgagc atgcgttcga aaaagttcag
     4681 ctttcgaaag aactggagcg gttgaaggaa gaggtcggcc ggaaatacgg tatcgatcat
     4741 attgtgggca aatcgaagca aattatagat attttcaaag cggtcagtat ggtagcggct
     4801 aagaaatcta ccgttctgat ccaaggcgaa agcggtactg gcaaggagtt gattgcacgc
     4861 gcaattcatt acaatagcga tcgccgttcg aaacccttca tcgttgtaaa ctgcgccgct
     4921 ttacccgata ctttaattga aagtgaattg tttggatatg aaaagggtgc gtttaccaat
     4981 gcatcacaga agaaaatcgg tcgcttcgag cttgcccatg gtggcacgct ctttctcgac
     5041 gaaatcggcg agcttaatct aagcacccag gcgaaatttc tacgcgccat cgagcaagaa
     5101 acgttcaccc gacttggcgg caccgatgac atcaaagttg atgtgagggt gatcgccgct
     5161 agtaaccggg atttggaaat cttggcaaaa gcgggtgagt tccgtccaga tctgttttac
     5221 cggctcaacg tcgtctccct ttatttacct gcccttcggg aacggcgcga ggacatcccg
     5281 ctacttctag atcatttcct gagactcaag gcgcaggagc attccattgc ggttaaaagc
     5341 ttgtcgccgg aagtagtcga cttttttacc gcataccatt ggccagggaa cattcgagag
     5401 cttgagaatc taatcgaacg gctgacgata ctaacgcccc atgatcagat catgctccgt
     5461 gatctgcccg tcgggatgcg ttcgacagat cataccgcga cgctgaaaga ggatgtcctg
     5521 agtggctcga gacctcttag cgaagctgtg gacgaattcg aacgggagct tattgtaaag
     5581 gcgctgcaac ggaccgggtt caatcaaacg aaagccgcct tactgctggg cactagtcgg
     5641 cgcatcctaa aatatcgaat tgaaaagctg aagataaatg agtctcatag cggcgacgag
     5701 ccgaagttat cgacataaat cgacaatttt ggacaaattt gtacctttaa tccttccaga
     5761 taacctctgt agccttcatt ttccttgtaa gttcttagtt ttctcgcgca aagcctatta
     5821 gcacgctctt tgcatcagga gccgtgcatg gattccaata agccgcacat tctggttgtc
     5881 gacgacgaaa tgggacctcg agaatcgtta aagatgattc ttaatcctta ttacacggtt
     5941 cacgtagcgg agcgcggagg tcaagccgtc gacttcttaa acaggtttcc ggtcgacctg
     6001 gttacattag atctcaagat gccgggtttc accggaatca atgttctcga aaaagtgaaa
     6061 cagcatgacc cggatatcga agcgattatc attacgggtt acggctcttt ggacacagct
     6121 atcgaaggac tccgcctcgg cgcattcgat tatatttcta aaccattcga tgtaaatcat
     6181 attttatctc ttgtccggcg cggtattgag cggcgcggcg caaagaccaa gctgcgacaa
     6241 gttagatcgg acttcctctc caacgtttca catgaattgc gaacgccgct aagcgttgtc
     6301 gtcggctttg tctatctatt gctcaaccag gttattggca aactcagcga agagcagcaa
     6361 aaggtcctcg aaaccgttta tcgcaactcc gaggaattgt tggaactgat cgacaacgtt
     6421 ctatggatga cgtcactcag ctctggcgac agctcaccaa tcatcgaaaa gttcgatgca
     6481 cgcgagatcg tagacgaaac ggtcaaacgt tatcagcgaa cgatcgggga aaaaaacttg
     6541 agcctgacga tcgacatccc agagggcggc gcatcgatca tcagcgatcg ctcgaaagtg
     6601 gaaagaatct ttcaaaacat attcaacaac gcagttaagt ttacctcggt gggcgagatc
     6661 cgggttaaag cccgtgtatc tgcggatcga ggagtgctgg acctacaggt cgccgatacc
     6721 ggaatcggca tcgaaaagag taagatggat tcagttttcg agccgtttca acaagttgac
     6781 aactccattc atcgttcgtt ctccgggctc ggcctgggtc taactgttgc gcggcgaatg
     6841 gccgagctga tcggcggtac aattcaggtc aaaagcgagc cgggtctcgg caccaccgtc
     6901 ctgatgcgat tcccttctaa ggctgccgcg cctgctgcag tgcctctgga gcagcgtaat
     6961 tatgcttagc gacaaattcc tttggtgtcg caggtgcgat gctgtgcatc acgtaacgtc
     7021 ctgcgataga gctcctcgct ataactacaa tgagtacacc gacagcgagc tcgatgagat
     7081 acccgccgac gattggcgtg ctttcatgaa agaacatgaa ggtcaccggt tagagccatt
     7141 agaagcatca ggcgaaaagt acttttgcca gggac
//
LOCUS       DASUVS010000104         2932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1345526, whole genome shotgun sequence.
ACCESSION   DASUVS010000104 DASUVS010000000
VERSION     DASUVS010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345526"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VF182_05845"
     CDS             complement(<1..458)
                     /locus_tag="VF182_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6436633.1"
                     /translation="MKWGSDVVAEVTRRLNLKYIALVPGASYRGFHDSIVNYLGNSNP
                     QMVICLHEEHAVSIADGYGKVTEEPMAVALHANVGLMHAAMPIFNAWCDRTPMIIFGA
                     TGPVDAHRRRPWIDWIHTAKDQASIIRDYTKWDDQPASPQAAVESVLRAN"
     gene            complement(711..1745)
                     /locus_tag="VF182_05850"
     CDS             complement(711..1745)
                     /locus_tag="VF182_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6436634.1"
                     /translation="MANDEPKGAVHWIDHYVVGTNDLPAWTHWALKATGLPIRPINGL
                     TTNMRKKNTPIFCFMWWEGGSCRIGAFLQPEVYPAAKELGKDLPRCGFYVRPEDIDLH
                     LCRLDQHKIPHTQPVKTAAEGEEGTVIYFADPDGNQYEFWAPVHMPEGAMEIATSERV
                     GRISHAVYGSRDLARTAAFFEKYCGVGVERNARTAEGTLVLRLRAGARLIYKLVEQVD
                     ERIAGHSPWWDMHTALTVREQEFFPNYRRMWEGLPEEEGPKETLKDSRDKEDAFPART
                     GLHRSPVGYRWKEIYQRGDEFYDWDGHAFHFFGGIPLKDDGSLALYQGKEQEEYLREL
                     AESLKTRGQA"
     gene            complement(1877..>2932)
                     /locus_tag="VF182_05855"
     CDS             complement(1877..>2932)
                     /locus_tag="VF182_05855"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6436635.1"
                     /translation="LPESLRERGPHVEDRGEKGEYWVCDGETWAEYRGERWFAKSNRT
                     RLALDRGGVGEAHRPTTPEKRLTDMDRDGVEASLMFPPIIAMQVGEPELRNACVRAYN
                     DWALEFGRAAPKRFFPVAMLSPVDAQDAADEVVRVAKLGFREANFLVNDVTLEMNLKP
                     WDVFWAAAEDAGIVISYHVGGSVQSGTVRATIDAMKPGERQGVFDMGLSNGATSFFNP
                     FVNMFNFGTLERHPKLRFCLAESAIGWIPFVVQEMDYRYRRQFERKKSEDIPLKNMPS
                     EIFKRQVWATYQTDLVGLHLIQFFGDGHIMWGSDYPHPDSTWPFSKEFIEKDSAHLTP
                     EMKRKVIRDNAAALYGV"
BASE COUNT          622 a          846 c          782 g          682 t
ORIGIN      
        1 tggttcgccc ttagcaccga ttccactgcc gcctgcgggg aagccggttg atcatcccat
       61 ttagtatagt cgcgaatgat cgatgcttga tccttggcgg tgtgaatcca gtcgatccag
      121 ggccggcgcc gatgggcgtc cacgggaccg gtggcgccaa agatgatcat cggcgtgcga
      181 tcgcaccagg cgttgaagat cggcatcgcc gcatgcatca gcccgacatt ggcatgtagc
      241 gccacggcca tcggctcctc ggtgactttg ccgtagccgt cggcaatcga aacggcatgc
      301 tcttcgtgca ggcaaatcac catctgcgga ttgctgttgc cgagatagtt aacaatgctg
      361 tcgtgaaatc cgcgatagct ggcgccggga accagcgcaa tatatttgag gttcaatctg
      421 cgcgtgacct cagcgacgac gtcgctgccc cacttcatct gcggcttgcc gagattctcc
      481 gggcggtcga tgctgctgtc cttggcttgc cgaatttttg cctgcgttgt ttctgacttc
      541 acggatctag gtctccttag gatgctaagc gaggccgctt agcatatatg aaaaaataaa
      601 aattaattga tgctgataac aactacgaag aacacgaagg ccacgaccgt tcagaagttc
      661 attttgagtt cgagatatgt cggtcgcttg cttcggatgt catcgaataa ttaggcctga
      721 ccgcgcgtct ttagagattc agccagttcg cgtaaatact cttcctgttc tttgccctga
      781 tataacgcca gcgaaccgtc gtctttcagc ggtatgcctc cgaaaaagtg aaaagcatgg
      841 ccgtcccaat cgtaaaattc atcgccgcgt tgatagatct ccttccatcg gtagcccacc
      901 ggacttcggt gtaaaccggt ccgtgcgggg aaggcgtctt ccttgtcgcg ggaatctttg
      961 agcgtctcct tcggcccttc ctcctcgggc aatccttccc acatgcgccg gtagtttgga
     1021 aagaactcct gttcgcgaac cgtgagcgcg gtatgcatgt cccaccaggg actgtgcccg
     1081 gcgatccgct catcgacctg ctcgaccagc ttgtaaatca gccgagcgcc ggcccttaga
     1141 cgcaacacca gcgtcccttc ggcagtcctg gcattacgct ccactcctac gccacagtat
     1201 ttttcaaaga aggcggcggt tctcgccaga tcacgggagc cgtaaacagc atggctgata
     1261 cggccgactc tctctgacgt ggcgatctcc atcgcgcctt ccggcatatg gacaggcgcc
     1321 cagaactcat actgattgcc atccgggtcg gcgaaataga tcaccgtgcc ttcctctcct
     1381 tcggccgctg tcttaactgg ttgggtatga gggattttgt gctgatcaag ccggcacagg
     1441 tgcaggtcaa tgtcttcggg acggacgtag aagccgcacc ggggaagatc cttgcccaat
     1501 tcctttgccg ccgggtaaac ctcaggctga aggaaagcgc cgatacggca cgatccgccc
     1561 tcccaccaca taaagcaaaa gatcggtgtg ttcttctttc tcatgttggt ggtcaggccg
     1621 ttgattggcc tgatgggaag gcccgtggcc ttaagcgccc agtgagtcca cgctggcagg
     1681 tcattggtgc cgacgacgta gtgatcgatc caatgaactg cgcctttcgg ctcatcgtta
     1741 gccataaatc ctccgtttac agaaagcggc tgtttcaatt cgaggtacta gactggagaa
     1801 acggtgtcaa gcggtcgtca aataacgagg ttcaagagtt caatagttca aggtcagagt
     1861 tgaacggcta ttttctttag actccgtaaa gcgctgcggc gttgtcgcgg atgactttcc
     1921 tcttcatctc cggcgtcaga tgagccgagt ctttctctat aaattcctta gaaaacggcc
     1981 aggtgctgtc cggatgcgga taatccgaac cccacatgat gtgtccgtcg ccaaagaact
     2041 ggatcagatg cagtcccacc agatcggtct gataggttgc ccaaacctga cgcttgaata
     2101 tttcactcgg catgttcttt agcggtatgt cttcggactt ctttctttcg aattggcgcc
     2161 tgtaacggta atccatttcc tgcacgacga acgggatcca gccgatagcc gattcggcaa
     2221 ggcagaatct caatttggga tgtcgttcca gagttccgaa attgaacatg ttcacgaatg
     2281 gattgaaaaa agaagtggcg ccgttgctaa gacccatatc gaacacgccc tggcgctcgc
     2341 ccggtttcat cgcgtcgatg gttgcccgaa ccgttccgga ctgcacgctg ccgccgacat
     2401 gataggagat tacgataccg gcgtcctcgg cagcggccca gaatacatcc caaggcttta
     2461 agttcatttc gagagtgaca tcattaacca ggaagttggc ttcgcgaaat cccagcttcg
     2521 ccacccggac cacttcatcc gcggcgtctt gagcatcgac aggggagagc atcgccaccg
     2581 gaaaaaatcg cttgggcgcg gctctgccga attccaacgc ccaatcattg tacgctcgaa
     2641 cacaggcatt tctcagctcg ggttcgccga cctgcatggc aataatcggt ggaaacatga
     2701 gcgaagcttc gacgccgtcg cggtccatgt cagtaagccg tttctcggga gtcgtcggcc
     2761 ggtgcgcttc accgacgccg ccgcggtcca gagccagcct ggtccggttg gatttggcaa
     2821 accagcgctc accacgatac tcggcccagg tctcgccgtc gcaaacccag tattccccct
     2881 tttcaccccg atcttccacg tgggggccgc gctcgcgcaa agactcgggc ag
//
LOCUS       DASUVS010000105         3298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1563985, whole genome shotgun sequence.
ACCESSION   DASUVS010000105 DASUVS010000000
VERSION     DASUVS010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563985"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..653
                     /locus_tag="VF182_05860"
     CDS             129..653
                     /locus_tag="VF182_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6436636.1"
                     /translation="MQARPLFYLAMITAVLFSTRLVAETAEIAETQIREGSQVALEYT
                     LSDETGTVIESNKGKQPMSYIHGKSQIIPGLEKELSGMKVGEEKKIQVKPEDGYGPVN
                     PDAFQEVPKDKLPPEALKVGAMLMAQGPQGQGIPVRVHEIKDTTVIMDFNHPMAGKTL
                     SFDVKISEIKTPEK"
     gene            complement(877..1659)
                     /locus_tag="VF182_05865"
     CDS             complement(877..1659)
                     /locus_tag="VF182_05865"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6436637.1"
                     /translation="MKTLLLAVSFFLLVISIVQAQTAFFEGKTIRIVTGYPAGDVNDM
                     WPRLIAQHMTKYIPGNPNFIIQNMPGASSMIAANYVFTVAKPDGLTLGWIAPTLYFDQ
                     LVGRKEVQYDWGKYSFIGSPAESEHHLYMRTDSPYKTIEDVRTANEPPKCGSGGAAGT
                     GFYFPRLLEETVGAKFTIVLGYQGGGPIDLAVEKGEIHCRAMTIESFFAREPFHTWRR
                     NNFVKSIAQSARKRDARLPDTPTIYELMDRYKTTDQSRVSPR"
     gene            1892..2800
                     /locus_tag="VF182_05870"
     CDS             1892..2800
                     /locus_tag="VF182_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6436638.1"
                     /translation="MPAKLKLVIFVAVLTVGIAFAARAQDFGPGFHKIKDGIYTFAPD
                     QLTTTCSFVITEMGVVMIDACNSPLDSHRMAAAIKKVSDKPIMFLIDTETHSDHTGNH
                     FIFSPPATVINAQGAAAGMKKEYNPKRAEALAAKSPEMREALKGEKLIPPHIEYKDRM
                     TINLGERTFELIYLKNAHSFADTAVWMPKERVLFASSAANVRTFINLRPTVVLPDVIS
                     SFKLMRSLNPEVVIAGHGQPTTPIIFDEYEGFYNLLMKRVGEMAAQGKSLDEIKKELK
                     MPEYADWQGQDRLEVNIDAAWRVVKK"
     gene            2839..>3298
                     /locus_tag="VF182_05875"
     CDS             2839..>3298
                     /locus_tag="VF182_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6436639.1"
                     /translation="MLITLTATKVILLALLSISYPSSSAVAQQEIVSLPTRAGVTQSY
                     FLTSIPKNLEAVALLFPGSGGLIQLRTENGKPRFNQGNFLVRSRADFIKRGVVAAILD
                     APSDQQNGWGMSDIFRLSDEHLRDMSVVTEDLAKRFPHLPLFLIGTSRGTI"
BASE COUNT          831 a          862 c          860 g          745 t
ORIGIN      
        1 cgccgccagc gaacatttgt gcttggcagt gccgaggtat tcgcctcgtt tcatatggtt
       61 gaattcccgg attgcaccac gtaggctcaa atactgcacg cggtctacag tctggaggag
      121 agaacctaat gcaagcacgc ccccttttct atttggcaat gattacggcg gttcttttca
      181 gcactcgtct tgttgccgaa actgcagaaa ttgccgagac gcagattcgg gaaggctcgc
      241 aggttgccct ggagtacaca ttgtccgatg aaaccggcac tgtgatcgag tcaaacaaag
      301 gtaaacagcc gatgagctac atccatggca agagtcaaat catcccaggg ctagagaaag
      361 aactttcagg aatgaaggta ggcgaagaga aaaagatcca agtcaaacct gaggatggct
      421 atggacccgt caatcccgat gcgtttcagg aagttccgaa agacaaactc ccaccggaag
      481 cgctgaaggt gggagcgatg ctgatggccc aaggtcctca aggtcaagga atcccggtgc
      541 gtgttcatga aatcaaggat acgacagtaa tcatggactt taatcatcca atggctggta
      601 agactctgtc cttcgatgtc aaaatctccg agattaagac ccccgaaaaa taactcctgc
      661 tggtgaattg aaacacggac gcgttctgtc gacttggcga gtttctccat gtcctcgccg
      721 gcaaccggat ccattcataa tttctttttt cagcttccgc tttgagctcg ggatctttga
      781 ccgcactcac aaatgcggcg cgaagaagtt tgagccgatc agccggcatt ccagcggggc
      841 cgaccatcgg ccggccgaac acaccacccg caaggatcag cgtggcgaca cgcgtgattg
      901 atccgtcgtc ttgtatcgat ccatcaactc atagattgtc ggcgtgtccg gcagacgagc
      961 gtcccgtttg cgtgccgatt gcgcgatgct tttgacgaaa ttgttcctgc gccaggtatg
     1021 aaacggctcg cgcgcaaaga atgattcaat cgtcatagct cgacagtgga tctcgccctt
     1081 ttccaccgca agatcgatag gtccgccccc ttgataccca agaactatgg taaatttcgc
     1141 tcccacggtt tcttccaata gcctgggaaa ataaaaaccg gtgccggcgg cgccgcccga
     1201 accgcatttg ggcggctcgt tggcggtgcg gacatcttcg atggttttgt acgggctgtc
     1261 ggtgcgcata tagaggtgat gttcgctttc cgctggggag ccgataaagc tgtacttgcc
     1321 ccaatcgtat tgcacttctt tgcggccgac gagctgatca aaataaagag tgggcgcgat
     1381 ccaacccagg gtaagcccgt ccggtttagc cacggtgaat acataattag cagcgatcat
     1441 cgagctcgct cccggcatat tctgaatgat aaaattcgga ttaccgggaa tgtacttcgt
     1501 catatgttgg gcgatgagcc gcggccacat gtcgttgaca tcgccggccg gatatccggt
     1561 gacgatccga atcgtcttac cttcaaagaa agcggtctgc gcctgcacaa tagagatcac
     1621 gagaagaaag aaagaaaccg ccagcaacag cgtcttcata gtaatcctcg gaacttacgc
     1681 acccgccaat gtttagagtg aatgtcgtac gtaattaagt gaatgtcgca cgtaattaaa
     1741 ggtctcggcc aacgcactca tagtacaaac aagacttatt ggtcaattgc atcattagcg
     1801 tttccggcga tcttagagaa tcagccttga cgcggtactg tgattgaact agcttcctgt
     1861 ccaagaaatc aatcgccaag gaggcagcag catgccggcc aaattgaagc ttgtcatttt
     1921 cgttgcagtc cttacagtgg gcatcgcctt tgccgcgcgg gcgcaggact tcggcccagg
     1981 atttcataag atcaaagacg gcatctacac tttcgcgccg gatcagctta cgactacatg
     2041 cagcttcgtg atcaccgaga tgggcgtcgt gatgattgat gcctgcaata gtccgttgga
     2101 ttcgcaccgc atggcggccg caatcaaaaa ggtaagcgac aaaccgatca tgttcttgat
     2161 cgataccgag acgcacagcg accacactgg taaccatttt atcttttcgc cgccggcaac
     2221 cgtcatcaat gctcaaggcg cggcagccgg gatgaaaaaa gagtataacc ccaaacgcgc
     2281 tgaggccctt gccgcgaaat ctcccgaaat gcgcgaagca ttgaaggggg aaaagttgat
     2341 tccgccgcac atcgagtaca aagatcgcat gaccatcaac ctcggtgagc ggactttcga
     2401 attgatctat ctgaaaaatg cccacagttt cgccgacacg gcggtgtgga tgcctaaaga
     2461 acgggtactg ttcgcgtcat cggcagccaa cgtgcgcacg tttattaact tacgaccgac
     2521 ggtggtgtta ccggacgtca tctccagctt taaactgatg agatcgctca atccggaagt
     2581 agtaatcgcc ggccacgggc agcccaccac tccgatcatc ttcgatgaat acgagggctt
     2641 ctataatctg ctgatgaagc gggttggcga aatggcggcg caagggaaat ctctcgacga
     2701 aatcaaaaaa gagctgaaaa tgccggaata cgctgactgg cagggtcagg atcgtctcga
     2761 ggtcaacatc gatgccgcgt ggagagtcgt gaagaagtaa caagataaag cggacttgtt
     2821 tcgaggcgaa ccaaggcgat gctcatcaca ttgacggcga cgaaagtgat tcttctcgcc
     2881 ctgttatcga tatcctatcc ttctagtagc gcggttgccc agcaagagat cgtgtccctg
     2941 ccgacacgag cgggggtaac ccagtcgtat ttcctaacca gcattccgaa aaatcttgaa
     3001 gccgtcgccc tgctatttcc cggttccggt ggcttgatac aactccgcac cgagaacggc
     3061 aagcccaggt tcaatcaagg taattttctc gtacgcagcc gcgctgactt cattaagcgc
     3121 ggggtggttg cagccatttt ggatgcgcca tctgaccagc aaaacggctg gggcatgagt
     3181 gacatatttc gtctcagcga cgaacatcta cgggatatgt cggtcgtcac tgaagatctc
     3241 gcaaagcggt ttccccattt accgttattt ttgataggaa cgagccgtgg gacgattt
//
LOCUS       DASUVS010000106        11450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_908696, whole genome shotgun sequence.
ACCESSION   DASUVS010000106 DASUVS010000000
VERSION     DASUVS010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11450
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908696"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="VF182_05880"
     CDS             complement(<1..1421)
                     /locus_tag="VF182_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX6436640.1"
                     /translation="MIQSDEEKNSAAAGGANDFEAMFEESLRSVKPGGIVRGMVVGIT
                     ATHVLIDVGYKSEGQIPIHEFQDRQGNLEVKVGEEVDVYFDSSENEGGGINLSRQRAQ
                     SMKVWEVIEKAFNEGTPVEGTIIGKVKGGFKVDVGGVMGFLPGSHVDIRPTRNLDKFL
                     GTKDSFAVLKFNRPRGNVVLSRRALLEKERDVLKQEVLKVLEEGVILEGTVKNITGYG
                     AFVDLGGIDGILHISDMSWGRISHPSELLQVGQKVNVVVLKFDSEKERISLGMKQLMP
                     DPWHSVAEKFPIGMRVQGKVISIMDYGAFVELESGIEGLIHISEMSWTRKIAHPSKIL
                     QVGQPVEVVILNVDPEHRRISLGLKQVMPNPWEEAKQNYPVGSVVKGPVRNITDFGIF
                     VGIEEGIDGLVHVSDLHWTKKVKHPSELFKKGDIVEAKVLGVNVENERFSLGIKQLAT
                     DPWKVIAERFPVGTRVKGQVTSVP"
     gene            complement(1418..2110)
                     /gene="cmk"
                     /locus_tag="VF182_05885"
     CDS             complement(1418..2110)
                     /gene="cmk"
                     /locus_tag="VF182_05885"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX6436641.1"
                     /translation="MIVAIDGPSGAGKSTLAKRIAKELRFTYLDTGAIYRALALKILR
                     RGVDLDDTAALNAIVNDSDIDLRGRNGQLEVFLDGEDVSAAIRTPEVSQMASKASAIA
                     MVRRRLLDLQRALGRRDNVVAEGRDIGTVVFPEAQVKIYLDASIEERARRRCDELRAA
                     GREVTLAETISEMEERDKRDSQRDIAPLRKADDAFALDSSGLTADGVAEKVLQIIKSE
                     SLKIKYDRSLGL"
     gene            complement(2147..3436)
                     /gene="aroA"
                     /locus_tag="VF182_05890"
     CDS             complement(2147..3436)
                     /gene="aroA"
                     /locus_tag="VF182_05890"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX6436642.1"
                     /translation="MMKRVLQSKKPLRGEISVPGDKSIGHRAVIFGSIAHGPSRVSNL
                     SCGEDNQRTVQAFKDLGVKISTQGETLHIEGKGWEELTAPKHIIDCGNSGTTMRLLCG
                     VLAGRPFRTVLDGDQSLRRRPMQRVIEPLTRMGAHLAGLEGTGLAPLEIDGTNLEGIE
                     YPMPVASAQVKSAILLAGLQAGGVTTVEEPQSSRNHTELMIKGFGGEVALNGRRVSIK
                     GGQTLHGMDVRIPGDISSAAFFLVAAALVPGSQVVVRRIGINPTRDGVIEILRRMGAD
                     IELLGAATEAGEPVADLKVTGGRLKGVEIGAELVARTIDEYPILAIAAALADGITVID
                     GVKELRFKESDRISAMTEGLRRLGVDVEEREDGMTIRGRETLQGNKVRSFGDHRIAMS
                     LAIAGLCADGGVEIDDAQCVDISFPAFFDLLDEICLP"
     gene            complement(3445..4308)
                     /locus_tag="VF182_05895"
     CDS             complement(3445..4308)
                     /locus_tag="VF182_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX6436643.1"
                     /translation="MAVMFHKMVVAGVGLICGSLAMDMRRHRLVGEIIGYGRSEDTLR
                     WAKKRGIIDSYFLREDEIPEGVDFLMMGTPVQTIVPLTATFLPSLKAGCVISDVGSVK
                     AEIVRGMERLLPKDIPFVPGHPIAGSEQWGARAAKPDLFLGRRTILTPTRRTDKAALK
                     KVTRLWRKVGAKVEHMDPEAHDRVLGLISHLPHVLVYALVNALDRSPVRGVDLKSYCA
                     GGFKDFTRIASSRPELWRDICLMNRQALDRSLGDYIDSLEQLRRWIAEGKGASLEKEF
                     ARANEIRQQIA"
     gene            complement(4362..5456)
                     /gene="hisC"
                     /locus_tag="VF182_05900"
     CDS             complement(4362..5456)
                     /gene="hisC"
                     /locus_tag="VF182_05900"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX6436644.1"
                     /translation="MKVIDYVPDYIRTLIPYAPGKPIEEVEREIGIANSIKLASNENP
                     LGPSPLALEAMGEKLAQLHLYPDGDCFYLKRGLTAKLNVAPNNLIFGNGSNEIIELAI
                     RTFMRAGDEAVMARQAFVVYKLVVQAVGGISREVPLRNFTHDLEAMSEAIGPKTRIVF
                     LANPNNPTGTIYRRANWEAFLKQIPPEVLIIVDEAYFEYVEAADYPNSLNYHRQDKAV
                     LTLRTFSKLYGLAGLRIGYGIANKEMISLMHRVRQPFNVNAPAQWAALAALDDREHVR
                     RSLENNRRGLEYLTAEFARLGIEYVPSHANFILLRVGQGQDVFSRLLAQGIIVRPMAG
                     YQLPEYLRVTIGTMDENRKFIDGLKKALGA"
     gene            complement(5453..6529)
                     /gene="pheA"
                     /locus_tag="VF182_05905"
     CDS             complement(5453..6529)
                     /gene="pheA"
                     /locus_tag="VF182_05905"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX6436645.1"
                     /translation="MSRNEPVDVLRKKIDQIDEKVVALLNDRALLAQKIGRSKSQNNQ
                     EIYVPHREKQILQRLSRLNQGPLPEHSIRAIFREVVSACRSVEAPLKIAFFGAEGTYS
                     HLAAREKFGSTVALLPTASISEVFQEVSQGRVSFGVVPIENSTEGVVAHTLDVLVDSE
                     LKICAEIYLEIHHHLLSRTGREQDIKRIASHPQALAQCRQWLTRHFPAAKLDEVASTA
                     HAAIVAAGDKSAGAISSALAKEAYDLQVIAANIEDHSNNITRFLVIGAQETDPSGDDK
                     TSLVFAVKDKPGVLHRMLQPFARTRINLTKIESRPIKNKPWEYRFFIDFKGHHKETRI
                     MRALGTLETSCDFIKILGSYPSGK"
     gene            6668..7567
                     /locus_tag="VF182_05910"
     CDS             6668..7567
                     /locus_tag="VF182_05910"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX6436646.1"
                     /translation="MVNQEIVVPAAETGKKLQTFLNSRFPIGYIRKLFRKNGLRVNGR
                     HSKPEDLVRAGDRIRLYIPFEKTIRRSATPSQARPKFSVLYEDTQVLVINKPAGIAVH
                     EGKRVLKRHSVIGALESTYREQGLSPRLVHRLDKDTSGVLLIAKDELTAGELEAIFAA
                     GKGAQKEYLCLVAGRLQDNQGKIDLPLAGREGAPVRALTRFTVLHRFSQTTLARVNIE
                     TGRMHQIRLHFAAIGYPIVLDDQHGDFNFNKQFSKKYGLKRQFLHACRLEINHHGKKH
                     VWSAPIPPDLQRVIDALRAEQAD"
     gene            complement(7599..8234)
                     /locus_tag="VF182_05915"
     CDS             complement(7599..8234)
                     /locus_tag="VF182_05915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6436647.1"
                     /translation="MIEFRPIGIEQYVERHSKPMSLFHDKIWLETYRQTGNPTTMVGP
                     LAAQLLKMLVLMTGARRILEIGMYTGYSTLAFAEALPKDGRVVTCEINPETTAIARQC
                     FAESQHGKKIEVKLGPALKSLRVISGPFDLCFIDADKDRYGEYFDASMQLVRSKGVIV
                     LDNMLRSGKVMHPSDPGSQAIDALNKRIRNDTRVENVLLPMRDGIMLVVKL"
     gene            8367..9317
                     /locus_tag="VF182_05920"
                     /pseudo
     CDS             8367..9317
                     /locus_tag="VF182_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006511891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            complement(9716..10534)
                     /locus_tag="VF182_05925"
     CDS             complement(9716..10534)
                     /locus_tag="VF182_05925"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEX6436648.1"
                     /translation="MPHNLRLTSFKRAALRGSCRLVPLSTARALALTLVGMISLSLTD
                     ARGASSQPPQEKTAVPVFTMPDESSLVVESIADGDSPLPMAAMIGAGGAKWFMVKTRN
                     GNVGWIKAGDHDSAKKVDSHFRSLPNENSVISLPQAASLGPAKGAGAIPVQVSGSRVI
                     VSVNFNNSTAANMLLDTGASRTMISSRIARSLGLQSTGSGKGYGIGGMVTVSTARVDS
                     VKLGEMERQNLLVMIHDFSPDPSYEGLLGFDFLSHFHMSLDLQKSLLVLTPRKG"
     gene            complement(10611..>11450)
                     /locus_tag="VF182_05930"
     CDS             complement(10611..>11450)
                     /locus_tag="VF182_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6436649.1"
                     /translation="VNASDPVTGVRVKTPQGTIHVAADLVVGADGRNSLIREKARLPV
                     QEIGAPMDVLWFRVPRNPNDPAQTMGRFDTGRIFISINRGEYWQCGYVIAKGRFDWIR
                     RQGLEAFRKEIAQLAPYAKDRVDELKAWEDVKPLTVRVDRLSQWFGPGLLCIGDAAHA
                     MSPIGGVGINLAVQDAVATANALARPLYDRRVRIKALARVQKRREFPTRLTQWLQVAM
                     QRRVIARVLRQTGPVKPPLLLQLIAAIPLFRRITARIIGLGIRPEHVRVPEWHAGTGK
                     SAT"
BASE COUNT         2613 a         3341 c         2863 g         2633 t
ORIGIN      
        1 ggaacactcg tgacctggcc ctttaccctc gtgccgacgg gaaaacgttc ggcgatgact
       61 ttccatggat cggtggctaa ttgcttgatg ccgagagaga acctctcgtt ttcgacattg
      121 acacccaata ctttggcttc aactatgtcg ccctttttga atagttcgga cggatgcttg
      181 accttcttgg tccagtgcaa atccgaaacg tgaaccaggc cgtcgatccc ctcctcgatg
      241 ccgacgaaga taccgaaatc ggtgatattg cgcactggtc ccttgaccac gctgccgacc
      301 ggataattct gcttcgcctc ctcccacgga tttggcatca cctgcttcaa acctaacgaa
      361 atgcggcggt gttccggatc gacgttcaga ataaccactt cgaccggctg cccgacttgc
      421 agaattttcg aagggtgtgc gatctttcgc gtccatgaca tctcggagat atggatcaag
      481 ccctcgatgc cgctttccag ctcgacaaaa gcgccatagt ccataatgct aatgaccttg
      541 ccttgaacgc gcattccgat aggaaacttc tccgccacac tgtgccacgg atcgggcatc
      601 aactgcttca tccccaacga gatacgttcc ttttcggaat cgaacttcag aacaaccacg
      661 ttcacttttt gaccgacctg aagaagttcc gagggatggc tgatccgccc ccaggacata
      721 tcgctaatgt ggagaatacc gtcgatgccc ccgagatcga cgaacgcgcc gtagcccgtg
      781 atattcttga cggtgccttc aagaatgaca ccttcctcga ggacctttaa tacttcctgc
      841 ttgagaacgt ctcgttcttt ctccagcagg gcccggcggg agagcacgac gttgccgcgc
      901 ggccggttga acttgagcac ggcgaaggag tccttggtgc cgagaaattt gtctaagttg
      961 cgcgtcggcc tgatatcgac atgcgagccg ggcagaaagc ccatcacgcc gccgacgtcg
     1021 actttgaagc cgcctttgac cttgccgata atcgttcctt cgacaggcgt tccctcgttg
     1081 aacgcttttt cgatgacctc ccagaccttc atgctctggg cgcgttgccg cgacaaattg
     1141 atgccgccgc cttcgttctc cgaggaatca aagtaaacgt caacctcttc gccgactttt
     1201 acttctagat tgccttgacg atcctggaac tcatggatcg gaatttgtcc ttcggatttg
     1261 tagccgacgt cgatgagaac gtgcgttgcc gtgataccca cgaccattcc cctgacgatg
     1321 ccgccgggtt tgacagaacg caggctctct tcgaacatag cttcaaaatc attagcgcct
     1381 cccgctgcgg cgctattttt ttcttcgtca ctctgaatca tagacccaaa ctccgatcat
     1441 attttatttt taaagactca cttttaatta tttgaagcac cttttcggcc accccatccg
     1501 cagtcagccc tgaggagtcc agggcgaagg cgtcatctgc tttgcgtaac ggcgcaatat
     1561 cgcgttggct gtctcttttg tctcgctcct ccatttcgct gatcgtctcg gcgagggtga
     1621 cctctcgtcc ggcggcacgc aattcgtcgc agcggcggcg ggccctctct tctatagatg
     1681 catcaagata gattttcact tgcgcctcag ggaagacaac ggtgccaatg tcccggcctt
     1741 ctgccacgac attgtcgcgt cgtccaagcg cgcgctggag atccaaaagc cggcgacgaa
     1801 ccatcgcgat ggcagacgct ttggacgcca tttgactaac ttctggagtt cgaatcgcag
     1861 ccgacacatc ctccccatcg agaaatacct caagctgacc atttctcccc cggagatcga
     1921 tgtcgctatc attcactata gcattcagtg cggcagtgtc atcgagatcg acgccccggc
     1981 gcaaaatttt caaggcaagc gcccggtaaa tggcgccggt atccaggtag gtaaaacgca
     2041 attccttggc aatgcgtttc gccaacgtgc ttttacccgc tccggaagga ccatcgatcg
     2101 ctacaatcat ttacgccccg cttgacctac taccgctaac ggactgttat ggcaaacaaa
     2161 tctcatcgag caaatcgaaa aacgccggaa acgatatatc gacgcattgg gcatcgtcga
     2221 tttccacgcc gccgtcggcg cacagcccag cgatcgccag tgacattgcg atccgatgat
     2281 cgccgaagct gcgcacctta ttcccttgga gcgtttcgcg gccgcgaatc gtcatgccat
     2341 cttcacgttc ctcgacgtcc accccaagcc tgcgcaatcc ttcagtcatg gccgagatgc
     2401 gatccgattc tttgaagcga agctccttta caccatcgat aaccgtaata ccgtcagcca
     2461 gcgcagcggc aatcgctaaa atgggatatt catcgatcgt gcgcgcgact agttcggcgc
     2521 caatctcgac acccttgagg cgtccgccgg tcacttttag atcagccacc ggttcaccgg
     2581 cttcggttgc ggcgcctagc agttctatgt ccgcccccat gcgccgaagt atctcaataa
     2641 caccatcccg ggtcggattt ataccaatgc gacgtacaac aacctgcgat cccggtacca
     2701 gcgcagccgc cactagaaaa aatgccgccg aggaaatgtc ccccggtatg cgcacatcca
     2761 ttccatgaag tgtttgaccg cctttgatcg aaacccggcg gccatttagc gccacttcac
     2821 cgccaaagcc tttaatcatt aattcggtat ggttgcgcga actttgcggc tcctcgacag
     2881 tcgttactcc gccggcctgc agacccgcca ataaaatagc cgacttcacc tgagcgctgg
     2941 caaccggcat tggatactcg atgccttcaa ggttcgtccc gtcgatctcg agcggcgcca
     3001 atcctgtgcc ttcaagacca gcgaggtgag cgcccatacg agtgagcggt tcgatcactc
     3061 tttgcatcgg ccggcgccgc agcgactgat cgccatcgag caccgttcta aacggccggc
     3121 cggccaaaac cccgcacagt aaacgcatgg tcgtgcctga gtttccgcaa tcaattatgt
     3181 gcttgggtgc agtcagctct tcccagcctt tgccctcgat atgtaaggtt tcaccttggg
     3241 ttgagatctt cacccccaga tctttgaatg cttgaaccgt gcgctggtta tcctcgccgc
     3301 aagaaagatt gctgacccga ctcggaccgt gagcgatact gccgaatata acggcgcgat
     3361 gcccaatgga tttatcgcca ggaacggaaa tttcgccgcg cagcggcttt ttactttgta
     3421 ggactcgttt catcatcccg agcgtcaggc aatctgttgg cgaatttcgt tggcgcgggc
     3481 aaattccttc tccaaagacg cgcctttacc ttcagcgatc cagcgcctga gctgctccaa
     3541 actgtcgatg taatcgccga gcgagcggtc cagagcctgc cggttcatca aacagatatc
     3601 gcgccacaac tcgggccggc tcgaagcgat acgagtgaaa tctttaaatc cgccggcgca
     3661 ataggatttc agatcgacac cccgaacggg cgaacggtcg agcgcgttca ccagagcata
     3721 gaccagaacg tggggcaaat gactgatcaa tccgagcacg cgatcgtgag cttccggatc
     3781 catatgctcg accttggcgc cgaccttgcg ccagagccgg gtgacctttt ttaacgcggc
     3841 tttatcggtt ctcctcgttg gcgtcaaaat cgtccggcgg cccaaaaaca agtcgggctt
     3901 tgccgcgcgg gcgccccact gttcgcttcc ggctatcgga tggccgggaa caaacggaat
     3961 atctttcggc agcaaccgct ccatccctcg aacgatttcc gctttgacac taccgacatc
     4021 gctgatcaca caacccgctt tcaagctggg gagaaacgtc gccgttagcg gcacgatggt
     4081 ttgcaccggc gtacccatca ttaaaaaatc cacgccctca ggtatctcgt cttcacgcaa
     4141 aaaataactg tcgatgatac ctcgcttctt agcccagcgc agagtgtctt cgctgcgccc
     4201 gtaaccgata atctcgccga ctaatctgtg ccggcgcatg tccatagcca atgacccgca
     4261 aatcagtccg acgcccgcca ccaccatctt gtgaaacata acagccatca aatttcctca
     4321 gccgataccg gcgcgatcca ttgatcatcg ccgctcttac gctacgcccc gagcgctttc
     4381 ttaagtccat cgataaactt tcggttttca tccatcgtcc cgatcgtcac cctcagatac
     4441 tctggaagct gatatcccgc catcggtcgc acaatgattc cttgagctaa caaccgacta
     4501 aaaacatcct ggccctgacc cacccgtaac aagataaaat tggcgtgact cggaacgtat
     4561 tcgataccca acctggcaaa ctctgcagtc aaatactcaa gtccacgtcg gttgttttca
     4621 aggctgcggc gcacgtgctc gcgatcgtcc aaggcagcca aggcagccca ctgcgccggg
     4681 gcattgacat tgaacggctg gcgcacccga tgcatcaagc tgatcatttc cttgttcgca
     4741 atgccataac cgattctcaa gccggcaaga ccgtaaagct tggagaaggt ccgcagggtt
     4801 aacaccgcct tgtcctgccg atgataattc aacgaattgg gatagtcggc ggcctcaaca
     4861 tattcgaagt aagcttcatc gacgatgatc aaaacctcag gcgggatctg ctttagaaaa
     4921 gcctcccaat ttgccctgcg gtaaatcgtt ccagtgggat tgtttggatt ggccagaaat
     4981 acgatgcgag tcttggggcc gatggcttcg ctcatcgcct ccagatcatg ggtgaagttc
     5041 ctcagcggca cttcccgcga gatcccgccc accgcctgca cgaccagttt ataaacaacg
     5101 aacgcctggc gcgccatcac cgcctcatcg cctgcgcgca tgaacgtccg gatcgccagt
     5161 tcaatgattt cgttggagcc attgccaaaa atcagattat ttggagctac attgagtttc
     5221 gccgtcaacc cgcgctttaa ataaaagcaa tcaccgtccg ggtaaagatg gagctgggct
     5281 aacttttcgc ccatcgcttc aagcgccagc ggcgagggcc cgagagggtt ctcgttggaa
     5341 gccagtttga tcgaattcgc gatgccaatt tcgcgctcga cctcctctat cggttttccc
     5401 ggcgcgtaag ggatcaaggt gcggatataa tccgggacgt aatctataac tttcattttc
     5461 cgctcggata agagcccagg atcttgataa aatcacagct cgtttcaagt gttcctagcg
     5521 ctcgcatgat cctggtttcc ttgtgatgac ctttgaaatc aataaagaac ctgtattccc
     5581 agggtttgtt tttaatcggt cgcgattcga ttttagtcag attgatccga gttcgcgcga
     5641 acggctgaag catgcggtgc aacacccccg gcttgtcctt taccgcaaac accagagagg
     5701 tcttatcatc gccgctggga tcggtctctt gggccccgat caccagaaac cgcgtgatgt
     5761 tgtttgagtg atcctcgata ttggcggcaa taacttgcag gtcataggcc tccttcgcca
     5821 acgcgctgga aattgcgcca gccgatttat cacctgcggc tacgatcgcc gcatgcgcgg
     5881 tgctcgccac ctcgtcgagc tttgcggccg ggaaatgacg cgttagccat tgccggcatt
     5941 gcgccagagc ttgaggatga gacgcaatac gcttgatatc ctgttcgcgc ccggtgcgtg
     6001 acaataaatg atgatgaatc tcgagataaa tctcggcgca gatcttgagc tcggaatcga
     6061 ccaagacatc gagagtgtgc gcgaccacgc cttcggtgga attctcaatc ggcacaactc
     6121 cgaacgagac tctcccctgg ctcacctctt gaaacacttc actaatactg gcggtcggga
     6181 gcagcgctac tgtggaaccg aatttttcgc gagcagccag atgggaatag gttccttcgg
     6241 ctccaaagaa agcgatcttg agaggcgctt cgacggaccg gcaagcggaa acgacttctc
     6301 tgaagatggc gcggatagag tgttctggaa gcggaccttg gttcaaccga gagagccgct
     6361 gcaggatttg cttctctcga tgcggaacat aaatctcttg attgttttgg ctcttgctgc
     6421 ggccgatttt ttgcgcaagc aacgcgcgat cgtttagcag ggcgacgact ttttcatcaa
     6481 tttgatcgat tttttttcgc agaacatcga ccggctcatt acgactcaaa gacaacctcg
     6541 ctggggcgcg cacccggcgc gcagactctt ttagaaatac cggcgtaacc tatcttaatt
     6601 cctaaagaat tgcaaatatt gccaaaatct tcgcttgacg aaccgagagg cgtgccgcag
     6661 attgatcgtg gtgaatcaag aaatcgtcgt gcctgcggct gaaaccggta aaaaactgca
     6721 aactttcctg aatagccggt ttccaatcgg ctacatccgc aaactttttc ggaagaacgg
     6781 tttgcgggtc aacggtagac attccaaacc cgaggacttg gttcgcgccg gtgatcgcat
     6841 ccggctgtat attccatttg agaaaaccat ccgccgcagc gctacgccat cccaggcgcg
     6901 accaaagttt tctgtgctct acgaagacac ccaagtgctg gtgatcaata agccggccgg
     6961 catagccgtt catgaaggga agcgcgtcct gaaacggcat tcggtcatcg gcgcgctcga
     7021 aagcacatat cgggaacagg gactttcacc ccgattggtg caccgtctcg ataaggacac
     7081 ctcgggagtt ttgctgattg ccaaagacga attaacggcg ggcgaactgg aagcaatctt
     7141 tgccgccggg aaaggcgcgc agaaagaata tctctgcctc gtcgccggac gtcttcaaga
     7201 taaccagggt aaaatcgatt tacccttggc gggtcgggaa ggcgcgccgg tgcgagcgct
     7261 tacacggttt acagtgctcc atcgattttc acaaaccacg ctggcgcgcg tgaacattga
     7321 aaccgggcgc atgcatcaga tccggcttca cttcgcagcg attggttatc ctattgtgct
     7381 ggacgatcaa catggggatt ttaacttcaa taaacaattc tccaaaaagt atggcctaaa
     7441 acggcagttt ctgcatgcct gccgactcga gatcaaccat cacggcaaga agcacgtttg
     7501 gagcgcgcca attccgccag atttgcagcg ggtaatcgac gctttgcgag ctgaacaagc
     7561 ggattagcag aatttcattt aatcactgaa cgattacttc agagctttac tactagcatg
     7621 atcccatcgc gcatcggcaa tagcacgttt tccacccgcg tgtcgttgcg aatacgcttg
     7681 ttcaatgcgt cgatggcctg actgccggga tcggatggat gcatgacctt gccgcttcgc
     7741 agcatgttgt ccaaaacgat cacacccttg gatcgaacta attgcatact ggcgtcgaag
     7801 tattcgccat aacggtcctt gtcggcatcg atgaaacaaa gatcgaatgg acccgaaatt
     7861 actctcaatg atttcaaagc cggtcccagt ttcacttcga tttttttgcc atgctggctt
     7921 tcggcgaaac actgtcttgc gatcgccgtt gtttcaggat tgatctcgca ggtgacaacc
     7981 ctcccatcct tcggtaacgc ttcggcaaaa gctaaggtgc tgtatccggt gtacatgccg
     8041 atctctaaaa tacggcgcgc gcccgtcatg agcacaagca tcttcaacag ttgagccgcc
     8101 agaggaccga ccatggtggt tggattcccg gtttgcctat aggtttccag ccagatcttg
     8161 tcgtggaaca acgacatcgg tttagagtgg cgttcgacgt attgctcaat gccgatgggc
     8221 ctaaactcaa tcaaagtctt tcccccttat gcaatcgaat tgctccgtcg cgcttgggaa
     8281 cgaaatgcta cggttggcct atcaaagttg actcggccca agcgtgtgct acctttacag
     8341 aacaattgtg aaaggatttc aagtcgatgg ccgaaacaaa tgatgaaaga gttccagtaa
     8401 ccgttttgac cggatttctc ggcgcgggca aaacgacact cttgaatcgc atattgaccg
     8461 ctgagcacgg ccggcgcgtt gccgtaatcg tcaacgaatt cggcgaggtc ggcatcgacc
     8521 atcatttgct gatttcctcg gaacaagaga tcgttgaaat gagcaacggc tgtatctgct
     8581 gcaccgttcg cggcgacctg ctgcgcagcc tattccagct gctcgagcac agatccaagt
     8641 tcgacactct gatgatcgaa accaccggcc ttgcggatcc ggcgccggtc gtacaaacct
     8701 tcttcgtcga cgaacgaatc aaagcccaat ttcaactcaa tggggtcgtc actgtggtcg
     8761 actccaagca tatcgcgcag cagatcgata actccaccga ggcccgcgaa caaattgcct
     8821 ttgccgacct ggttttactt aacaaactcg atctcatcag cccggaagat ctgcaggagc
     8881 ttgagtacaa aatcaaaaac ttgaatcggg cggcgcgcat ttaccagacg cgtaactccg
     8941 acgtggaaat cgccaaagtt ttagatcttc gcagtctcga gatcgaagcc agggcgcaag
     9001 agcatgatga cgagcacaag cacacccagg acatctccac tgtttcaatc gtcacgcccg
     9061 gggatctcga cggggtcaag ctcagccaat ggtttcgcca actgctagcc gagttcggcc
     9121 aaagtatcat gcgcatgaag ggaattctca atcttcgcaa cgacccggac cagtttgtat
     9181 tccagggagt tcatctcttg ttcgaaggcc gtcccggacg cgcttgggcc gaaggcgagg
     9241 aacgtaccaa tcgattggtg ttcatcggcc ggaatctcga tggtgaaaag atcaccaatg
     9301 gatttcaagc ttgcctcacg gccgaggacg gcaacgggtc cgctcaagtc gacccgttcg
     9361 gccaaaaaat cgatgtttca agctttgttt tcgatcagat ccgttattgg gtgcagacga
     9421 ttctagaatt tccgcccgac gcccccatcg tcatcaaaga agtgccctgc gtcaaagaag
     9481 gttgccctcc agtggagacc gcgattatgg ttttcctcaa aaacgaaccg ccgcgagtgt
     9541 ttaaaattct ggcgcgagtc cacgaagtca ccttcgatca cgtttataac ttgattgaaa
     9601 acccgctgcc atgctgctga tggcgtcgat tccatttaca attaagtacg atgctcgtag
     9661 cgtttactcc caccgttcgg aaagccgccg cgtgacgtag cctccggctt cgtccttagc
     9721 cctttcgcgg cgtcagcact agaagcgatt tctgcaaatc cagtgacata tggaagtggc
     9781 tcaggaaatc aaatcccagt aaaccctcat agcttggatc aggagaaaag tcatgaatca
     9841 tcactagcaa gttctgcctt tccatttccc cgagtttgac cgaatccacc cgcgctgtcg
     9901 agacagtcac catgccgccg attccatagc ccttgccaga gcccgtcgat tgaagcccaa
     9961 gactcctggc gatccgcgaa gaaatcatgg tgcgggaagc gccggtatcc aataacatgt
    10021 tagcggcagt gctattattg aaatttacag aaacgatcac tctcgaaccg ctcacctgaa
    10081 cgggaatagc gcctgcgcct ttcgccggcc ctaaggaggc cgcttgtggc aggctaataa
    10141 ccgaattttc attaggcaag gaacgaaaat ggctgtcgac tttctttgcc gaatcatgat
    10201 cacccgcctt gatccaaccg acgttaccgt tccttgtctt taccatgaac catttcgcgc
    10261 caccggcgcc gatcatcgcc gccatcggca atggagagtc tccgtctgca attgactcca
    10321 ccaccagcga tgattcgtcc ggcatcgtaa acaccggtac ggccgttttc tcctgcggcg
    10381 gctggctcga tgcgccgcgg gcatcagtca ggctcaaaga aatcatccct accaaggtaa
    10441 gcgccaaggc gcgcgccgta gacagtggaa ccagcctgca ggaaccgcgc agcgcagctc
    10501 gcttaaagct ggtcaatcgt aaattgtggg gcatgatcct aatcccttcc tgaagtcgtt
    10561 ggttgacatc gccgggtaag acccgggcga tttattgagc ctgatcccac tcacgttgca
    10621 gattttcccg tgcccgcgtg ccattcgggg actctcacat gctcagggcg aatgcccagt
    10681 ccgataatgc gcgcggtgat gcgccggaat agtggaattg ctgctatgag ttggagcaac
    10741 agcggcggct tcaccggtcc ggtttgtcgt aacactcgtg caatcactct gcgctgcatg
    10801 gccacctgta accattgtgt cagccgcgtt ggaaattcac ggcgcttctg aacccgcgca
    10861 agcgccttta tgcggacacg cctgtcgtaa agcggtcggg caagcgcatt cgccgtcgcc
    10921 actgcgtctt gaacagcgag attgattccg actccgccga tgggcgacat ggcatgggcg
    10981 gcatcgccga tgcagagcaa gcccgggccg aaccattggg acaagcgatc gacgcgaacg
    11041 gtcaagggct ttacgtcctc ccaggccttg agctcatcga cgcggtcttt ggcgtagggc
    11101 gctagttggg caatctcctt gcgaaacgcc tccaggcctt gtctgcgaat ccaatcgaac
    11161 cggcccttgg cgatgacgta gccgcactgc cagtattcgc cgcgattgat gctgataaaa
    11221 attcggccgg tatcaaagcg acccatggtt tgtgccggat cgttcgggtt gcgtggcacg
    11281 cgaaaccaga ggacgtccat gggcgcgccg atttcctgca ccggtaatcg agctttctcg
    11341 cgaatcagcg aattccggcc gtcagcccca accaccagat ctgccgccac atggatggtt
    11401 ccttgcggtg tcttcacgcg aacgccggtt acaggatctg aagcgttaac
//
LOCUS       DASUVS010000107         6516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1565335, whole genome shotgun sequence.
ACCESSION   DASUVS010000107 DASUVS010000000
VERSION     DASUVS010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565335"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1244
                     /locus_tag="VF182_05935"
     CDS             225..1244
                     /locus_tag="VF182_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6436650.1"
                     /translation="MPTVKFFSIIWLSAMIVLPGAIYGQDAKLVEGAKKEGGKVVIYG
                     SIENDTMDLIAAALKKKTGLETEFWRSSSTKVMDRVLNEMRTGKPLYDVVLTNTAPME
                     FMFNEGALAKYDSPSAKSFPKEVMHPNLGPQYRNSVIGIVYHAGIIKPAEAPKSLEDL
                     LKPQYAGKLVIPDASQHTTTAQWMASLHKVMGGKEKADKFIRDLAATKPLLVESLTPA
                     GERITTGERPLGIAFLKNVVFYGKKGVPLDYVRLGKFMGDGQAVAIAAKAPHPNAGKA
                     FVDFFIGEEGLKLMAGIGEFVTRKGIYPPLPDADKIELVEMIDMDKKAYAEKMAEYRK
                     IFLQN"
     gene            1465..3039
                     /locus_tag="VF182_05940"
     CDS             1465..3039
                     /locus_tag="VF182_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6436651.1"
                     /translation="MTDSEWDVILIGAGQNNFALGTYLGRAGLRTVICESRLENGGRL
                     ASEEITIPGYWHNTLAYFQDNREISPVWKELKWDSGYHAEFVSPPVISSLLLADGRSV
                     SHHQTLQDTLNSIARFSPNDADVWRQTHERFHPLIRDCLIPYYYHAPIPDSALDTRLE
                     TEPHGQEFRRLWHMTPDRALGELFENDAVKTLVLSQMAIPRGVAVDYPGGGIEVLKLI
                     AGDEKPELARGGSHSIAQVLQRAYVHAGGQIRAVHHVDKILVEEGKAVGIRLRDGREW
                     KAKLAVVSNTDPYLTFKELIGDEHLPRAFTERIEELEADEFSYFQIHLALKAPIRYAF
                     HEAADPAVARAMNVNIGAEKPDDLNEMWKEIRAGQFPQHVCLHAICPTVFDPLQAPRG
                     KHTASVFIPVPYQLEGKKPADWVKLKKGFMETVLDIWRRYATNLTDDNIEMKVAMDPF
                     YIAGRWPNMRRGSVWVARKSAAQMGANRPNERLSDYRTPIAGLYQVGVATHPADAVIA
                     GSGYNCWRVMKEDLKL"
     gene            3234..4856
                     /locus_tag="VF182_05945"
     CDS             3234..4856
                     /locus_tag="VF182_05945"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6436652.1"
                     /translation="MPKILEYDGVVIGAGHNGMICAGYLAKCGQKIMVVEKNMEVGGG
                     LDSHEDRDYPGFWHNIHSVFHRGLMMLPWYKDLELENFGIHYYKPDPGVVHHFIDETY
                     LGWFADVNRTAETIARFSKKDAATFLDIWSRWQPVVQNIVFPETYAVPLAFEEKRPLL
                     ERIAEGREYLKYFDTTPEEFVLRHFDHPRVQAFIGFLGVMRGYELDAPKTGYLIPAMI
                     AWGVNPQLCRGTSHALGDNLAHMMSHNGIDYVESNGVERILVDKGQATAVVLEDGTVI
                     KARKFIASNVNPVETFIKLVGREKLEPKFAEMANHFRFSKTTPIFATNLALNERPKYI
                     TEKKYPEVAGSFMHIVGLEAYDELRNLFEDCRTGELPRKPFMNGATPSFHDPTQAPPG
                     KATAFMWQLATYNLWGNPQNWDKVRGEWFDKQLAVWRRFAPNLDESNILSTDATTPLD
                     IERHDKNMYCGDWMIGEYAGDQALENRPFPGWGNYRTPIDGLYLCGSSCHPGGNITGA
                     PGYNAARVIAESLGLETWWKPVDMREHLNGLR"
     gene            4951..5694
                     /locus_tag="VF182_05950"
     CDS             4951..5694
                     /locus_tag="VF182_05950"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX6436653.1"
                     /translation="MRLNGRVAIVTGGARHIGAAYSRKLAEEGAAVVIADILDGEPVA
                     DEIRAAGGKALALKIDVANEADTRRMALETVKAFGRTDILVNNAAIFINIQRHPFYEI
                     SAEEWDRVSAVNIKGPFLCAKAVFPQMKEQKSGKIINISSSTAYWGTPNFLHYVASKA
                     ALIGMTRSLAREVGEFGICVNAIAPGLVEHEGQNAPKALTELQLKERSIKRLQTPDDL
                     MGTVVFLCSSDSDFMTGQTIVVDGGSVFH"
     gene            6042..>6516
                     /locus_tag="VF182_05955"
     CDS             6042..>6516
                     /locus_tag="VF182_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015824307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6436654.1"
                     /translation="MSEHAGHHFLQIPGPTPVPERVLRAIAAPVIDHRSPEFGRLARQ
                     VLDGCRQVFKTSSPVIIYPSSGTGAWEAAIVNTLSPGDRVLMVETGHFSVLWREMAER
                     WGVEVDFIPGDWRHGADPSAIEAKLAEDRLRTIKAVMVVHNETSTGVTSRIPEIRK"
BASE COUNT         1505 a         1702 c         1906 g         1403 t
ORIGIN      
        1 atgatcaata agtgatgcga agagagcagc gcggccaacg cgccgtcgat cagatccagc
       61 cgctcgagaa aagtttgccg caactcctcg cggatcttct tgagcttttc ctgtccagtc
      121 atgctctcga taaagttacc aaatggccct ggtagaaaca aatagacatc cgccgatcat
      181 ttcgatattg agggagcatt gcgtgtcaca cggagggttc gcttatgccg acggtaaaat
      241 tcttctcgat catctggctg tcagcgatga tcgttctgcc cggcgcgatc tacggtcagg
      301 atgctaagct ggtcgaaggc gccaaaaagg aaggcggcaa ggttgtcatt tatggatcca
      361 tcgagaacga caccatggat ctgattgccg ccgcgctcaa gaagaagacc gggctggaaa
      421 ctgaattctg gcgctcctcc agcaccaagg tcatggaccg cgtgcttaat gagatgcgca
      481 cgggcaagcc gctctacgat gtggtgttga ccaatactgc gccgatggag ttcatgttca
      541 atgaaggagc actggccaag tacgattcgc cgtcggcaaa atcatttcca aaagaagtga
      601 tgcatccaaa tctcggtccg caatatcgca atagcgtgat cggcattgtt taccacgccg
      661 gaatcatcaa acccgcggag gcgcccaagt cgctggagga tctgctcaaa ccacagtatg
      721 caggcaagct ggtaatcccc gatgccagcc agcatacgac cacggcgcaa tggatggcca
      781 gcctgcacaa agtgatgggc ggcaaagaaa aggccgacaa atttatccgc gaccttgctg
      841 ccacaaaacc cttgctagtg gaatctctga ctccggcggg agagcggatt acgacgggcg
      901 aaagaccact aggcatcgcc tttctcaaaa atgtggtgtt ctatggtaaa aagggtgtgc
      961 cgttggatta cgtgcggctc ggcaaattca tgggcgacgg acaagcggta gccatcgccg
     1021 ccaaagcgcc gcatcccaat gcgggcaagg cctttgtcga tttctttatc ggcgaagagg
     1081 ggttgaaatt gatggcggga atcggcgagt ttgtcacccg caaggggatc tatccgcccc
     1141 tgccggatgc cgataagatc gaactcgtcg agatgatcga tatggataaa aaagcatacg
     1201 ccgagaagat ggccgagtat cggaagattt ttttgcagaa ttaattgtaa tccgacgata
     1261 gttggtaccc tcatcagtga gggaggtcag cgagaccgga agcggtgggc accgaacgcg
     1321 cagttgactc tagcctcgac gtacgaaaca acatccttgg ggtatgcggg gcggccttca
     1381 aggcactgtc tcattgctcc ggctgcatgg ctcgctggcc tggcgggtta gaataatgtc
     1441 ggatcaggat taactggagg cgctttgact gacagtgagt gggatgtaat tctgatcggc
     1501 gccggccaaa acaacttcgc ccttggaact tatcttggtc gcgccggttt gcgaacggtg
     1561 atttgcgaga gccgcctgga aaacggtgga cgtttggcca gcgaagaaat cactattccc
     1621 ggttactggc acaataccct ggcttatttc caggacaatc gcgagatctc cccagtatgg
     1681 aaagagctca aatgggacag cggctatcat gccgagttcg tttcgccgcc ggtaataagc
     1741 tccttattgc ttgccgatgg ccgctcggtt tcccatcatc aaacattgca agatacgctt
     1801 aactcgattg cgcggttctc gccgaacgac gctgacgtat ggcggcaaac ccatgaaaga
     1861 ttccatccgc tcattcgcga ttgtttgatc ccgtactatt accacgcgcc gattccggac
     1921 agcgcgctcg atacgaggtt agagaccgag cctcatggcc aggaattcag gcggctttgg
     1981 catatgactc ccgatcgcgc gcttggcgag ttattcgaga atgacgcggt gaagactctc
     2041 gtgctgtcgc agatggcgat tccacgcggc gttgccgtgg attatcccgg cggtggcatc
     2101 gaagttctca aattgattgc cggcgatgaa aaacccgagc tggcccgcgg cggatcccat
     2161 tccattgcgc aagtgctgca gcgtgcctac gttcatgccg gcggtcaaat ccgggccgtg
     2221 catcacgtcg ataaaattct agttgaggag gggaaggcgg tgggaattcg cctgcgcgac
     2281 ggccgcgagt ggaaggcaaa gctcgccgtc gtctccaaca ccgatccgta tttgaccttc
     2341 aaagagttga tcggggacga gcatctcccc cgtgccttca ccgaacggat cgaagaactc
     2401 gaggccgacg agttttctta tttccagatc cacctggctc tcaaggcgcc gatccgctat
     2461 gcgtttcatg aagcggccga tccagccgtg gcccgggcca tgaacgtcaa cattggagcg
     2521 gagaaacctg acgacctaaa cgagatgtgg aaagagatcc gcgccggtca gtttccgcag
     2581 catgtctgtc ttcatgcgat ttgcccgacg gttttcgatc cgctccaggc gccccgcggc
     2641 aagcataccg cgtcggtctt tatcccggtg ccttatcagt tggaaggcaa aaagccggcg
     2701 gattgggtca agctcaagaa aggattcatg gagacggttc tggatatctg gcgacgttac
     2761 gcgaccaatt tgaccgatga caacatcgag atgaaagttg ccatggatcc attttacatc
     2821 gccggccgtt ggcccaatat gcgccgcggc tcggtgtggg tggcgcgcaa atcggcagcg
     2881 caaatgggag caaaccggcc caacgaacgc ctgtctgatt accgcacgcc gatcgccggt
     2941 ctctaccaag tcggcgttgc cacccatccc gctgatgcgg tgatcgccgg ctccggatat
     3001 aattgctggc gggttatgaa ggaagactta aaactttaag aaattgtttg ttcaaaacgt
     3061 tcaagctgtt ttagtagttc aagccattaa cgaatcgtcg gatgttcaaa ctgttcataa
     3121 agtcgattct tcaaactaca cgaagtgatc gggccatggg tttttctttg agagcgattt
     3181 gaactcttga acgtttgaac gatttgaaca gaattgcgaa gcagagataa acgatgccta
     3241 aaatcttgga atacgacggt gttgtaatcg gcgccggcca caacggcatg atctgtgccg
     3301 gctatttagc gaagtgcggc cagaaaatca tggtggtgga aaaaaacatg gaggtgggcg
     3361 gcggtctcga ctcgcacgag gaccgcgact atccgggctt ctggcacaac attcattcgg
     3421 tgtttcatcg gggcctgatg atgcttccat ggtacaaaga tttggaattg gagaatttcg
     3481 gcatccatta ctacaagccc gatccgggcg tggtgcatca ctttatcgat gaaacctatt
     3541 tgggctggtt tgccgatgtg aatcggacgg cggaaacgat tgcgaggttc tcgaaaaaag
     3601 acgcggctac gtttctcgat atctggagcc gctggcagcc ggtggtgcaa aatattgttt
     3661 tcccggaaac ctatgcggtt cccttagcct tcgaggaaaa gcggcccttg ctcgaaagaa
     3721 tcgccgaagg tcgcgagtat ctgaaatact tcgacactac gccggaagaa ttcgtgctgc
     3781 gccactttga ccatccgcgg gtgcaggcgt ttatcggttt cctgggcgtg atgcgcggtt
     3841 acgaactgga cgcgccaaaa accggttatt taatccccgc gatgattgcc tggggagtca
     3901 atccgcaact ctgccgcggc acatcccacg ctttgggaga caacctggcg cacatgatgt
     3961 cgcacaacgg catcgattat gtcgagtcca acggcgtcga acgaattctc gtcgacaagg
     4021 gacaagcgac cgcggtggtg ctcgaagacg gcacggtcat caaggcgcgc aaattcatcg
     4081 cctcgaacgt caatccggtg gagacattta tcaagctggt cgggcgcgaa aaactggagc
     4141 cgaaatttgc cgagatggcg aaccatttcc gtttttccaa gacgacaccg atctttgcga
     4201 ccaatctggc cttgaacgaa cggccgaaat acatcacgga aaaaaaatac cccgaggtcg
     4261 ccggttcgtt catgcacatc gtcggcttgg aagcatacga cgagctgcgc aacttgttcg
     4321 aagactgtcg tacgggggag ttgccgcgca agccattcat gaacggcgcg accccttcgt
     4381 ttcacgatcc gacccaggcg ccgccgggca aggccactgc atttatgtgg cagctggcga
     4441 cttataatct ctggggcaat ccgcaaaact gggacaaggt acgcggcgaa tggttcgaca
     4501 aacagctggc ggtttggcgg cgctttgccc ccaacctgga tgaatccaat atcctttcga
     4561 ctgatgcaac caccccgttg gacatcgaac gccatgacaa gaacatgtac tgtggcgatt
     4621 ggatgatcgg cgagtacgcc ggcgatcaag cactggaaaa tcgtcccttc ccaggatggg
     4681 gaaattatcg aactccgatt gacggattgt atctatgcgg ctcatcgtgc catcccggag
     4741 gaaatattac cggcgcgcct ggatacaacg ccgcccgggt gattgccgag tccctcggtt
     4801 tggaaacctg gtggaagccg gttgatatgc gcgaacacct gaacgggctc agatgaataa
     4861 tcggagtaat ggagcacaga agtactggag tattggattc cggagtcatc actccaccac
     4921 ttcattactc caatactcct ggaggggccg gtgaggttga atgggcgcgt ggcgatcgtc
     4981 acgggcggcg cgcgccatat cggggcggcg tactcccgca agctggccga ggaaggcgcg
     5041 gcggtggtca tcgccgatat tctcgatggc gaaccggtag ccgacgagat ccgtgcggcg
     5101 ggcggtaaag cgctggcgct caagatcgat gtcgccaacg aagcggacac acgccgaatg
     5161 gctttggaga cggtgaaggc tttcgggcgg accgatattc tggtgaacaa tgcggcgatc
     5221 tttatcaaca tccagcgtca cccgttttat gagatcagcg cggaggaatg ggaccgcgtt
     5281 tcggcagtca acatcaaggg cccattcctg tgcgccaaag cggtttttcc gcagatgaaa
     5341 gagcagaaga gcggcaagat catcaatata tcctcttcga ctgcgtactg gggaacgccc
     5401 aactttttgc attacgtcgc ttccaaagct gcgctgatcg gcatgacgcg atcgctggcc
     5461 cgcgaagtgg gcgagtttgg catatgcgtt aacgccatcg cgcccggtct ggtcgagcac
     5521 gaagggcaga acgcgcccaa ggcgctgacc gaattgcaac tcaaagaacg ctcgatcaaa
     5581 cggctacaga ctccggatga tttgatgggg actgtggtgt tcctctgttc gtcagacagc
     5641 gacttcatga cgggtcagac gattgtggtg gatggcggga gcgtctttca ctgaaagtct
     5701 ggttgaagca ggaaactaaa ttgcagcgat ggtctgtgtc gaccaagaca accttgagcc
     5761 tcgggagcgc tagagcatgt tgggatagat tagctcgtcc ggtgctgttg acgggaacag
     5821 tttcatgtgg acgcagttat gctccgcggt gtggcccaga attgctcatc atgcgatgag
     5881 gaaaacggca gccggcggta cctgccggat cttcgagccg cgatgccggc ttggcggaag
     5941 cgtccgggtt cctcatggcg atacgatatg attgcgtcca tgaattttga gaagcaatat
     6001 tacagccgca gcgcggtcgc tccgcctgct cggagcggat tatgagtgag catgcaggac
     6061 accatttttt gcagatcccg ggtcccactc cagtgccgga acgtgtgctc cgcgctatcg
     6121 ctgcgccggt aatcgatcac cgcagtccgg aatttgggcg gcttgccaga caagtgcttg
     6181 acggctgccg tcaggtgttc aaaacatcga gcccggtgat catctatcct tcctcaggca
     6241 cgggcgcctg ggaggcggcg attgtcaata cgctctcgcc aggcgatcgg gtgctgatgg
     6301 tggagaccgg gcatttttcg gtgctgtggc gagaaatggc ggaacgttgg ggggtcgagg
     6361 tcgattttat tcctggcgac tggcgtcacg gcgccgatcc gtcggcgata gaggcaaagc
     6421 ttgcggaaga tcggttgcgc acgatcaagg cggtcatggt cgtccacaat gagacctcca
     6481 caggtgtgac cagtcgcatt ccggaaatcc ggaagg
//
LOCUS       DASUVS010000108        12541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_254609, whole genome shotgun sequence.
ACCESSION   DASUVS010000108 DASUVS010000000
VERSION     DASUVS010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12541
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254609"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VF182_05960"
     CDS             <1..882
                     /locus_tag="VF182_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6436655.1"
                     /translation="WSPSVISLSTLSLTFFTASSIHASAMKDDFWIYIGGTLLALLVA
                     CAIFAPKLAPHDPSRQNLGNDLQVFTIEHPLGTDKLGRDILSRTLFGSRISLLVGVST
                     VAISISIGLFIGSISGYFGGWLDQLLMRAVDILLAFPGILLAIAFTALLGPGLDHVII
                     ALCLIGWTGYARLVRGEILSLRAQEYVHAARALGCTPARVIVVHLLPNLLPPLLIQAT
                     FGLAAAIIAEGSLSFLGLGVEPPTASWGSMLNEGRQFLLVAPHLTTYPGLAIMLTVLS
                     LNLLGDALQNRLRSSGS"
     gene            1077..1250
                     /locus_tag="VF182_05965"
                     /pseudo
     CDS             1077..1250
                     /locus_tag="VF182_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
     gene            1287..1598
                     /locus_tag="VF182_05970"
     CDS             1287..1598
                     /locus_tag="VF182_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX6436656.1"
                     /translation="MQRAKARIWVDFLNSRVHPAASDITHDREPDRARQHLQQHLNTL
                     ESEMTGKRFIVGDYSLADVSFVPFYARRQRYGVALDDNFPNLRRWGEELLARPAVACT
                     L"
     gene            1641..2696
                     /locus_tag="VF182_05975"
     CDS             1641..2696
                     /locus_tag="VF182_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6436657.1"
                     /translation="METLLVRPHEIRGIVTMAEAVEAVRTGFREWGENPQLNAPRRRI
                     HIPTGVRVSVHQGGVPAAGATGLMTHCEWVKPMAEEQIYPRLNHPVIVLYDAAEGELK
                     GIIIGEITCSELPNNIAVTGLRTAATSAVGTDLLARQDASTLGLFGGAGQAKNHLLAL
                     MQVRKLKDVKVYSRNPENRKRFAEEMGPLTNLNITPVSSPQETVRGVDIILAATNSSV
                     PVFDGNWLEPGQHVTTIVGSNVGLVKGGFTAAKRREIDDATLARSHVRGIVSVQQAVQ
                     DEQADIFDPVNRGVIKWEQLIEIGAILAGQDDGRTRADQITLYKNNAGQGVADVALGA
                     LVLEKARKKQAGQNLEV"
     gene            2791..3606
                     /locus_tag="VF182_05980"
     CDS             2791..3606
                     /locus_tag="VF182_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX6436658.1"
                     /translation="MSKVAIVQIQSSTEKQENLRKALELVKDSKSAGADLVVFPEFLM
                     AFSPANQTAKELAEIAEPLGGPFITSLKEAAKATHSAIVATIYESCSVADRVYDTAVW
                     IDNAGNVAAVYRKLHLYDAFGFRESDKFAAGGDLPPPVTLEDGCYGMMICYDLRFPEM
                     ARMLTLSGANVLLAPSGWVQGELKVDHWQTMIRARALENGCYVIAPDQVGNIYIGHSM
                     VVDPLGRTVVNFNQREAVEIIEIDSKLINDTRANLPLLQNRRTDIYRNHLSFG"
     gene            complement(3636..4577)
                     /locus_tag="VF182_05985"
     CDS             complement(3636..4577)
                     /locus_tag="VF182_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6436659.1"
                     /translation="MADRYLITGGAGFIGSHLVKHVLGTGGIVRIVDNLSTGSTERLN
                     QVRDSVQLLTGDLSDNSVAAEAVKGVDYVLHQAAVPSVQRSVVDPISTNRSNITATLN
                     LLENSRKAGVRRFVYAASSSAYGDTEVLPKNEEMPANPLSPYALQKWVGERYCKLYHE
                     LYGLETVSLRYFNVFGPAQDPYSEYSAVIPKFTTKLLAKEAITVYGDGEQSRDFTYID
                     NVVDANLLALRAPNAPGEVCNIGCGQRVTLNELIRILEELLGVKAQVIYAAAKPGDVR
                     HSLADITKAARVLGYVPKTEIEEGLRKTIEAYVKSND"
     gene            complement(4915..6129)
                     /locus_tag="VF182_05990"
     CDS             complement(4915..6129)
                     /locus_tag="VF182_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6436660.1"
                     /translation="MNLTYREHFGLKQEPFNITADPAFLYLSRSHREALAQLSYGIKA
                     RKGFVVLTGEVGTGKTTLINGLLDELDSDTRTALIFSHIANPFDLLRYTCNEFGLRSP
                     QHSRDDFYEYLALLNDYLFESYRSGKNCALIIDEAQNLSSEVLESVRLLSNFETSKDK
                     LLQILLVGQPELSSRLNSQELRQLKQRVTVRYQLRSLTLKECQAYISTRLQVAGGDPV
                     LFTPSAVEVIYGCSAGIPRVINVICDAALMTAYGLRKSYIDDSLIRQVAEDLQIAGDV
                     FSGEAASRRTNNPTSPIANEIPVQTQEHTNSTAQRPAIVKAVASLETRSGVVPRIVFD
                     ELSVILVDAMGPMASIVLRDRAASLGESLDRFPEDKLKTLIQLVSDEILDPSMRQDFQ
                     VLVQERVMPRVD"
     gene            complement(6413..7696)
                     /locus_tag="VF182_05995"
     CDS             complement(6413..7696)
                     /locus_tag="VF182_05995"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6436661.1"
                     /translation="MMKQAKEHTVGGLTQTLYNRESMEGIDAPEKSDAIQPRAHILII
                     DNEIRPREDLACFYTSIGHTVEIAESGEEGLNRVNNKKIDVVILYRDLISYGGREVLR
                     ELERDHPEVSVIMTGYMPIDYYDQDLQYGNYDYMAKPFQAEIIHNKTRNALNKSYVFA
                     ESTSLRQALKDTEGDFGLLLSKTPAMHQIFETMRMASPTNMSVVLEGEAGTGKELIAR
                     AIHYQSSRRSGPFVAVNCAAYPDALLETELFGCEDGNYFKEELNRPGKIKLADKGTLF
                     LDEIESMAPFVQSKFLRALEDWNSLHLGDQQSLDSDVRVITASNVPLQQLIAQGKMRA
                     DLYYRINVVPIHVLPLRERAGDIPLLIDDFLRWHPIAIQKHIGGVSPETIARLIQYRW
                     PGNIRELQNVLEKAVVLARDSMIDNIDLPATIRVN"
     gene            8262..9797
                     /locus_tag="VF182_06000"
     CDS             8262..9797
                     /locus_tag="VF182_06000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6436662.1"
                     /translation="MTLWLTVLLIAGCFRFVSLSEQSLWTDEVQTIEIARGSLSEVAL
                     SAAQTNIQPPLFFWLVRAMSAFGSNEFNSRLPSAIAGLLTVPLVWAIAAQITSRVTAM
                     MAMLAIAISPFHIWYSQEARGYAVLLFFLTTSVCCLLAWWRGVKKARYGYLLSTVLAL
                     YTHILALPFLAVQLMMAYFLSKHPPYSSPDSIVTLTFHRFIRTLSAAFLFLIPALVVL
                     FRQSYVLYLVPPFSLVLEGPNKVSMSMIAAGVLYSIYTLVAGFSLGPSTRELHSVTRF
                     GDLGAYITPGLAIFWIILGTLLVCGIMEAYRERVLRIAVLGWLVLLLALIAGAAFWAE
                     AVLYPRFVIAAMPPVCLLLGLGIAAQQTLTRKLLALIAFIMAMALPLRSASSDPRYAK
                     EDIRSAERYLSTVATEGDLIIISTGYMSRVLAHYCDRSRACKADMIAYPATDRRTTAE
                     GISAEVPKLIHGKGRVWLLLSRTFHSDPQGLLVQYFRISHAIKVRGSWSGVTLYEVKQ
                     KTG"
     gene            complement(9763..11316)
                     /locus_tag="VF182_06005"
     CDS             complement(9763..11316)
                     /locus_tag="VF182_06005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6436663.1"
                     /translation="MKLYDPTPGGVANVSSIPTEPTGANRKILVVLVGLGALTATFYF
                     EWWLRAPVGRLAFIVLPLTLFYHAAQIFIPWTLFLNARRAPAPPPQRGLSVDVFVVAY
                     KEPAWLVKKTLKAAVNMQYPHRTFLLDDGRRSELASAARALGAGYITRSNNKHAKAGN
                     INHALEQTDGDIIVIFDTDHTPKPEFLLRSLGHFRNSRVGFVQVMQTFSNRKESFISR
                     AATETTDEFFGPICIGMDSWDSCTLFGTNALIRRSALVEIGGYQPGLTEDLATSIELH
                     AAKWGSRYVHEPLAPGLSPSDLRAFFVQQFKWALGVFGLLFTVYPRRFFNMTLKQRLC
                     YLTRMTCYLAGPYCAAHILLAVFLLFGSGRTHDVFLDYALRYLPLFAVILTIQAYSVH
                     HWSREPARLRWRAISLVYSTWPVYVAALCCAIIRWKVDYLPTPKVFQAGNHLPLAAPQ
                     LFAAAGLLVGLTWLMNASSFTPSSVAIGAFAALLVLANASLSVGVIEGFQSRPIKRAS
                     QFSVLPRTR"
     gene            complement(12085..>12541)
                     /locus_tag="VF182_06010"
     CDS             complement(12085..>12541)
                     /locus_tag="VF182_06010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6436664.1"
                     /translation="LPDVELIKVGRAHFEREREHLIKLADQLGVRTAIHFVEEVSEDD
                     LVSLYNLAEVCVMPSLYEGFGFPVLEAMACGTLVVYANAGSLPEIAGTAAICVSPCDA
                     RSLAGALLDVLDERANKVISKAHRRAQAARFTWSATVKNTAAVYRRLLS"
BASE COUNT         2973 a         3260 c         3249 g         3059 t
ORIGIN      
        1 tggtcgccat cggttatctc attgtcaact ttatcactga cgttttttac agcgtcgtcg
       61 atccacgcat cagctatgaa agatgatttt tggatctaca ttggcggcac gcttttggcg
      121 ctgctggtag cctgcgcgat atttgcgcca aagcttgcgc cccacgatcc gagccgacaa
      181 aacctgggaa atgacctaca ggtgtttacc atcgaacatc cgttaggaac tgataaactg
      241 ggtcgggaca tattgagccg aaccctattc ggcagccgca tttcattgct ggtcggcgtc
      301 agtacagtag ccatatccat ttcgatcggc ctcttcatcg gttcgatctc cggctatttt
      361 ggcggctggc tcgatcagtt gctcatgcgt gcggtggaca ttctgctggc ttttcccgga
      421 attcttctcg ccattgcctt tactgcccta ctgggcccgg gcctcgatca cgtcatcatt
      481 gcgctttgtt tgatcggctg gaccggttac gcgcggctgg tgcgcggtga aattctcagt
      541 ttaagagcac aggagtatgt ccacgcagcg cgtgctctag gctgcacgcc ggcccgagtc
      601 atcgtcgttc atttgctccc caaccttctt ccgccacttc tcatacaagc gacgttcggg
      661 ttagcggcgg caataatagc cgaaggaagt ctaagcttct taggtttggg cgttgaaccg
      721 ccgaccgcct cgtggggttc gatgctgaat gaaggaagac agtttttact ggtggcgccc
      781 catctgacaa cctatccggg cttggcaatc atgcttacgg tcttgagtct caacctcttg
      841 ggcgacgctt tgcagaatcg actacgctca agcggcagct aaatccgttc taaagttcca
      901 tccttcagcc gttcggttgc tttattggat ccgcgaaaac cttcgaaatg ttgaaacgac
      961 tcagaccgga ttccgttgac tttgattgcc atcatcactg ggcaatgata ttgaaccatc
     1021 ggaaggaggg tgccgccgtg aaactttatt cgtgccatac gtgaccctat gcgcacagga
     1081 caaggattgt cctttccgag aaaaagattc cctttgagct cgtcgaagtc gatttgaaaa
     1141 ataaaccaaa ggatctcctc gagctgaatc cttatgccaa agttcccgtg cttgtcgacg
     1201 gcaccggagt gatttatgaa tccgccatca tcaacgaata tctcgatgag aaataccccg
     1261 aactaccgct gctcccggcc aatgcgttgc agagagctaa agcgaggatt tgggtcgatt
     1321 tcctaaacag tcgcgttcac ccggcggcaa gcgatattac ccatgataga gagcctgacc
     1381 gagcgcggca acatctgcag cagcatctca acacactgga gagcgaaatg acgggcaagc
     1441 gtttcatcgt cggtgactat tcgctggccg atgtttcttt tgttcctttc tatgcaaggc
     1501 gccaaagata cggcgtcgcc ttggacgata acttcccgaa cctaaggcgc tggggagaag
     1561 aactcctggc tcgtcctgcg gtggcgtgca cattgtagcg ctactctaat catcgaaccg
     1621 ccaccgacag gagaatagaa atggaaacct tgctcgtccg tccgcacgaa atcagaggca
     1681 tcgtgacaat ggcggaagcc gtcgaagcgg tgcgcacagg ctttcgtgaa tggggggaga
     1741 atcctcagct aaatgcgccg cggcggcgga ttcacattcc caccggtgtg cgtgtaagcg
     1801 tgcatcaggg tggcgttccg gcagcgggag cgacgggcct aatgacgcat tgcgaatggg
     1861 tcaaacccat ggccgaggag cagatttatc cgcggctcaa ccatccggta atcgtgctct
     1921 acgatgccgc cgaaggcgag ctcaaaggaa ttattatcgg tgagatcacc tgttcggaat
     1981 tgcccaacaa cattgccgta accggcttgc gcacggcggc caccagcgcg gtgggaaccg
     2041 atttgttagc gcgtcaggac gcttcaaccc ttggtctttt cgggggcgcc ggtcaggcca
     2101 aaaaccatct gcttgcgctc atgcaggtac gcaagctcaa ggacgtgaag gtttatagtc
     2161 ggaatccgga gaaccgcaaa cgcttcgccg aagaaatggg acccttgaca aatctcaaca
     2221 tcacgccggt atcatcgccg caggagacgg ttcgtggagt agacattata ttagcggcaa
     2281 ccaactccag cgttccggtg tttgacggca attggctcga gccaggccag catgtcacga
     2341 cgatagtcgg cagcaacgtc ggtttggtga agggcggctt taccgccgcc aaacggcgtg
     2401 agatcgatga cgccaccctg gcccgcagtc acgtgcgtgg catcgtttcc gtgcagcagg
     2461 cagtccagga cgagcaagcc gatatcttcg atccggtaaa ccgcggcgtc attaaatggg
     2521 agcaattgat tgagatcggc gccattctcg ccggccaaga tgacggccgc acccgagccg
     2581 accagattac tctttacaag aacaacgccg ggcaaggcgt agccgatgtg gctttaggag
     2641 cgctggtgtt agagaaggca aggaaaaagc aggcgggaca aaaccttgag gtgtagaggc
     2701 gtggaaatag tgaggagtag agcctgtctg gaacggaagt gaccgcaaga tgctgatagc
     2761 agaaaacgtc tacggaaagc agattcctct atgagcaaag tggccatcgt gcagattcag
     2821 tcgtcaaccg aaaaacaaga aaatctgcga aaagccctcg agctggtcaa agatagtaag
     2881 agcgccggcg ccgatctggt ggtctttccg gaatttttaa tggcgttctc acccgccaat
     2941 caaacagcca aggagctcgc cgaaatcgcc gagccgcttg gcggtccatt cataacatct
     3001 ctgaaggaag cggctaaagc gacgcattca gcgattgtgg cgacaattta cgagagctgc
     3061 tccgttgctg atcgagtgta cgacaccgca gtctggattg acaatgccgg aaacgtcgcc
     3121 gcggtttacc ggaaactgca tttgtatgat gccttcggat tccgggagtc cgacaaattt
     3181 gccgcgggcg gcgatttgcc accgccagtg accttggagg atggctgtta cggcatgatg
     3241 atttgctatg atcttcgttt ccctgagatg gcgcgcatgc tgactttatc gggcgcgaac
     3301 gtcctgctcg cgccttccgg ctgggttcaa ggcgaattga aagtggatca ctggcaaacg
     3361 atgattcgtg cccgagctct ggaaaacggc tgttacgtga tcgcgccgga tcaagttggc
     3421 aatatctaca ttggtcacag catggtcgtc gatccattag gccgcacagt cgttaatttt
     3481 aaccagcgtg aggccgttga aatcattgaa atcgactcta agttaatcaa tgatacccgc
     3541 gcaaatctgc cactcctgca gaatcgcaga accgacatct accggaacca tttatctttc
     3601 ggttgaagaa ttcacgactt aagcgctccc tcgcactaat cattcgattt tacgtatgcc
     3661 tctatcgttt tccttaggcc ttcctctatt tcggttttag gaacgtatcc gagaacgcgt
     3721 gcggccttgg taatgtcggc taaggaatgg cgtacatcgc ccggcttagc ggcagcgtaa
     3781 atgacctgcg ccttaacccc gagaagttct tccaatatac gaatcagctc gttgagggtc
     3841 acgcgctgac cgcacccgat attgcaaact tcaccgggcg cattcggtgc gcgtaatgcg
     3901 agcaaattgg cgtcgacaac attatcgata taggtgaagt cgcgggactg ctcaccgtct
     3961 ccataaacgg tgatagcctc cttggctaaa agcttggtgg taaacttagg aataactgcc
     4021 gagtattccg agtaaggatc ttgcgcaggg ccgaagacgt tgaagtacct caggctcaca
     4081 gtctcgaggc cgtaaagctc atgatagagt ttgcagtagc gctcccctac ccatttctgt
     4141 aaggcgtagg gagatagcgg atttgccggc atttcttcat ttttgggcag aacctccgtg
     4201 tcaccgtaag ctgaagagga agccgcatag acgaaccggc gaactcccgc tttccgagag
     4261 ttttccagga gatttagcgt ggctgtgata ttggaccgat tggtactgat gggatcgaca
     4321 accgatcgct gcacagacgg aacagcggcc tggtgtagaa cgtagtccac gcctttgaca
     4381 gcttctgcag caacggaatt atcggacaag tcaccggtta ggagctgcac cgagtcacgg
     4441 acctgattga gtcgctcagt ggagccagtg gatagattat ccacaatccg aacgatgccc
     4501 ccggtaccga ggacatgctt caccagatga gagccgataa aacccgcacc accagtgata
     4561 agataacggt ctgccatgcc ttacaccgcc cttttctgtg ttcgcgtcga gcctaaggtt
     4621 tactgcatgg aaggaattat aacaggcatc acggagcatg catacgaggt gaaagccttt
     4681 atgtgagcga tttgccacgg gccgcgaccg aatagaagtc agtcgacgac aacaccgagc
     4741 tcgtgcgaat acgcacacac tcactcacag cagcgccgca gcttgcgtcg ctgtatagtt
     4801 cgggaagtcc gatcgtggag cgatgtgcag gccggtttcg agggaatcgg gatatttgct
     4861 tgattctggg cgcgtgattc gacagacgag gctcagctgt cttgttattg cattctaatc
     4921 gactcgcggc atgacgcgtt cctgaacgag tacttgaaaa tcttgccgca ttgatgggtc
     4981 caatatctcg tcactaacga gttgaatcag cgtttttagc ttatcctctg gaaatcgatc
     5041 gagcgattcg ccgagactag ccgctcgatc cctcaatact atcgatgcca ttggccccat
     5101 cgcgtcaacg agtattacgc tcagttcatc gaaaactatt cgaggtacaa cgccgctccg
     5161 tgtttctaaa gatgctactg ctttcactat cgcgggtcgt tgcgcagtcg aattagtatg
     5221 ctcctgggtt tgtactggga tctcatttgc gattggcgaa gttggattat tcgtgcgcct
     5281 gctcgcagcc tcgcctgaga agacgtctcc agctatctgg agatcttccg ccacttgccg
     5341 gattaaggaa tcatcgatgt aggacttcct taaaccatac gctgtcatca gagcagcatc
     5401 gcagatcaca ttgatgaccc tcggaatccc cgctgagcac ccgtagataa cctccaccgc
     5461 actaggcgtg aaaagcaccg ggtcgccacc ggccacttgc aatctcgtcg aaatatatgc
     5521 ctggcattct ttcaaagtaa gagaacgcag ctgatagcgg accgttactc gctgtttcaa
     5581 ttgccgtaat tcctgcgagt taaggcggct tgataattcc ggttggccga caaggagaat
     5641 ctgcaatagc ttgtccttgg acgtttcaaa gtttgacaac agccgcacgc tttccagaac
     5701 ctctgatgag agattctgtg cctcatcgat gatcaacgca cagttttttc cagaacgata
     5761 actctcaaac aagtaatcgt tgagtagagc caggtattcg taaaaatcat cccgcgagtg
     5821 ctgcggcgat cgaaggccaa actcgttaca ggtatagcgc agcagatcaa atgggttggc
     5881 gatgtggctg aaaatgagag cggttcgggt atcgctgtcg agttcgtcca atagtccgtt
     5941 gataagagta gttttaccgg tgccaacctc gcccgttaaa acgacgaaac ctttacgggc
     6001 tttaatgcca taagagagtt gagccagagc ctcgcgatgg cttcggctca gatacagaaa
     6061 agccgggtcg gcggtgatat tgaacggttc ttgttttagt ccaaaatgtt ctctgtaagt
     6121 caaattcatg aaagctcgtt tcacgggtgc gtgccaggtt ctgcacacct cgcggctgag
     6181 cagattagca atattatacg aaaaaaatgt gttccaaagc tagtttcgga agtagcgagt
     6241 cttgcaactg agcaccgttt catacggaat cttaacctcg atgccatccg tttagtagct
     6301 tgcgccccga atttgcgaag cgctcaggcg atgtttccgt aaaatcgcac agccgcttgg
     6361 cccatagaag cgagcaaaat tatcgacgga ttcacccgag attacttgca tgtcagttaa
     6421 cccgtatggt cgcgggtaaa tcgatattgt caatcataga gtctctggcc agtacaacgg
     6481 ccttttccaa cacgttctga agttcacgaa tattccccgg ccatcgatac tgaataaggc
     6541 gggctatcgt ctcaggcgat acgccaccaa tatgcttttg aattgcaatt gggtgccaac
     6601 gaaggaagtc gtcaatcagg aggggaatgt caccggcccg ttcgcgcaaa ggcaaaacgt
     6661 gaatcggaac aacgttgatc cgataataca gatccgctcg cattttcccc tgggcaatca
     6721 gctgctgcag aggaacgttg gaggcggtaa tgacacgcac gtcactatcg agactttgct
     6781 ggtcgcctaa atgtaaactg ttccagtcct ccagcgctcg cagaaatttc gactggacga
     6841 acggcgccat actttcaatc tcgtccagga acaaagtccc cttgtcggct agtttgatct
     6901 ttcctggccg attgagctct tccttgaaat aattaccgtc ttcgcaacca aagagctcgg
     6961 tctctaacaa cgcatcaggg tacgcagcac aattaacggc aacgaagggt ccgcttcgcc
     7021 gggaactttg gtagtgaatg gccctggcaa ttagctcttt cccggtccca gcttcacctt
     7081 cgaggaccac gctcatattc gtcggtgaag ccattcgcat ggtttcaaaa atttgatgca
     7141 ttgcaggcgt tttactgagt aagagcccaa agtcgccttc agtatccttt agcgcctggc
     7201 gaagacttgt gctttcagcg aagacgtacg atttattcag ggcgttccgg gttttgttgt
     7261 gaataatctc ggcctgaaaa ggcttcgcca tatagtcgta attcccgtac tgcaaatcct
     7321 gatcgtagta atcgatgggc atatagccgg tcattataac ggagacttca ggatggtcgc
     7381 gttctaactc tcggaggact tctcttccac cgtaactgat gaggtcacgg tacaagatta
     7441 caacatcgat ctttttgttg ttaactcgat tcagtccttc ttcacctgac tcggctattt
     7501 caactgtgtg accaatggag gtataaaaac acgcaagatc ctctctcgga cgaatttcgt
     7561 tatcaataat gaggatgtgc gccctgggtt gaatggcatc ggacttttct ggcgcatcga
     7621 taccctccat agattcacgg ttgtaaagtg tttgcgtaag gcctccgact gtatgctctt
     7681 tggcttgctt catcatgttt ttctcaattg tacgagacat ttcctgaacg gagcgttccg
     7741 agatcgatcg gctacttcta tgagcaaacg tcagagacga aaactcaaag aaaagccgct
     7801 tctggtggat tgatcaaagc gatgtgctag cacctgttcc caagacgata acgcttacta
     7861 tttaacagtt tagcggtctc aaatcggtac aagtcgcgct gcttggtatc cttcgaaatc
     7921 cgctttatca agattgaatg gcatagcagg cggaatggaa ttaatcgaca aactgggttc
     7981 gatcggatcc ggggtgtctg tgtgtttcgc gttgcggcat cagcttactt aagatatttc
     8041 tagacaaaca gaatcgaccg cctcaggaca atgttgtgat tcagaagctc accaattctt
     8101 atatgcgata cgctcgctta tgcgactgat ttacgggaat cacattgcgt aggtccgcgc
     8161 tccgcgtccc ggtagctcaa gcgttacgcg ccacgcgaac gcgctctaaa gtgaaagaga
     8221 acgaagactt tccgatcgac cagcgctcga gaacgattta cgtgacactc tggcttacag
     8281 tcctgcttat tgctggatgt tttcgtttcg tttccttatc agagcaaagc ctgtggactg
     8341 acgaggttca aacaattgag attgcccgcg gctcgttgtc ggaggtcgcg ttaagcgcag
     8401 ctcaaactaa tattcagccg ccactttttt tctggctggt tcgcgcaatg tcagcctttg
     8461 gatctaacga gttcaacagc cgacttccct cagccatcgc cgggctcctt actgttccat
     8521 tggtttgggc gatagcggca cagatcacat cccgagtcac agcaatgatg gccatgttgg
     8581 ccattgcaat ctcccctttt cacatctggt atagccagga ggcgcgggga tacgcagtgt
     8641 tgctgttctt tctaacgacg tccgtatgtt gtctgctggc atggtggaga ggtgtaaaga
     8701 aagctcgtta cggctacctt ctctccaccg tattggcgtt gtacacccac attcttgctc
     8761 tgccatttct ggccgtgcag ctcatgatgg cgtatttcct ttctaaacac ccaccataca
     8821 gctcgcctga ttcgattgtc acgctcacat ttcaccgctt tatacggacc ctaagcgccg
     8881 cattcttgtt cctgatcccg gccctggttg tcctatttcg tcagtcgtac gttctctatc
     8941 ttgtgccgcc gttctcgctc gtcctggagg gccccaacaa ggttagcatg tcgatgatcg
     9001 ctgctggagt gctttacagc atctacactc tcgttgccgg tttttccttg ggtccgtcta
     9061 cccgcgaact gcatagcgtc acgcggttcg gagacctagg ggcatatata acacctggcc
     9121 tcgctatctt ctggatcatt ttgggaactc ttttggtatg cgggataatg gaggcttatc
     9181 gtgagcgtgt gctgaggatc gctgtgctcg gttggctcgt gctcttgtta gccttaatcg
     9241 ccggcgctgc cttctgggct gaagctgtcc tctatccgcg atttgtaata gctgcgatgc
     9301 ctccagtctg ccttctgctc ggactcggca tcgcagcgca acaaacactt actcgaaagc
     9361 ttctcgcgtt aattgcgttc atcatggcta tggctctacc gctccgaagc gcctcctccg
     9421 atcctcgata tgctaaagaa gatatccgtt ctgcggagcg gtacctctca acagtcgcga
     9481 cagaaggaga tctgattatc atcagcacgg gctatatgag cagggttcta gcacattact
     9541 gtgatcgcag ccgcgcctgt aaagcggata tgattgcgta tcctgcgacc gatcgtcgga
     9601 cgactgcgga aggaatttca gcggaggtac cgaagctcat acacggaaaa ggtcgtgtct
     9661 ggcttcttct ttcgaggaca tttcacagcg acccacaggg gcttctcgtt caatactttc
     9721 gtatcagtca cgcaatcaag gtccggggaa gctggtccgg cgtcaccttg tacgaggtaa
     9781 aacagaaaac tggctagctc gctttatcgg tcgcgactga aaaccctcga taactccaac
     9841 actaagcgat gcgtttgcca gtacgagaag cgcggcaaac gccccaatgg caacgctact
     9901 cggtgtgaag ctcgacgcat tcatcagcca agtcaagccg actagtagcc cggccgctgc
     9961 aaaaagctga ggagctgcga gtggcaagtg gtttcccgcc tggaaaacct tcggtgtcgg
    10021 caaataatca accttccaac gaattatcgc gcagcacagc gccgcaacgt agaccggcca
    10081 ggtgctgtac acaagtgaga tcgcgcgcca gcgaagacgc gctggttctc ggctccaatg
    10141 atgtacggag taggcctgaa ttgtcaggat cacagcgaat agaggaaggt agcggagtgc
    10201 atagtctaga aagacgtcat gggttcggcc gctaccaaac aaaagaaaaa ccgcgaggag
    10261 aatgtgcgcc gcacaataag gaccggcaag gtagcacgtc atccgtgtca gatagcagag
    10321 ccgctgcttc aaagtcatgt tgaaaaaccg tctgggatat actgtgaaaa gtagaccgaa
    10381 aacaccaaga gcccacttga attgttgtac gaagaaagcc ctaagatcgg agggtgataa
    10441 cccgggagcc agaggttcat gaacatatct agagccccac ttagccgcat ggagttcgat
    10501 agaggtagca aggtcttccg ttaatcccgg ttgatagcct ccgatctcaa ccaatgcact
    10561 ccgacggatg agcgcattgg ttccgaacaa tgtgcacgaa tcccacgaat ccatgccgat
    10621 gcagatgggg ccaaaaaact catccgtggt ttcggttgca gctcggctaa taaagctttc
    10681 cttcctgttt gagaaggtct gcataacctg cacgaagccg acccgggaat ttcggaagtg
    10741 gccgagcgaa cggagtagaa actccggttt cggcgtgtga tccgtatcga agatgacaat
    10801 gatgtccccg tcggtttgtt cgagagcatg attgatgtta cctgctttgg catgtttgtt
    10861 attcgagcga gtgatataac ccgcccccag tgcccttgct gccgaagcca gctcactgcg
    10921 cctgccatcg tctaagagaa aggttctgtg ggggtactgc atgttcacgg ctgccttgag
    10981 cgtctttttt acgagccaag ccggttcttt ataagccaca acaaagacgt cgaccgaaag
    11041 ccctcgttgg ggcggtggag cgggagcgcg acgcgcgttc aggaacaatg tccagggaat
    11101 gaatatctgt gccgcatggt aaaacagcgt cagcggtaag actatgaagg cgagtcgacc
    11161 aaccggcgca cgcagccacc actcgaagta gaaggtggcc gttaatgcac cgagcccgac
    11221 tagtacgact aagatttttc gattagcacc tgttggttcc gtcggaatcg atgagacgtt
    11281 ggcaacgcca cccggtgtag gatcgtagag cttcatagag aaatcttgct gcctccttcc
    11341 atttcttgcg cgtgtgaggg tcaaaactgt tgtaagcaag tgcccgcaga tctcctgcag
    11401 actttctcat tggccggcac tgtgttatta ccagcatgtt gcgatttcct gatctaaaat
    11461 cagctctgtg gggagacaag aatctcctca agagagccca tgcatgagat ggtcagcagc
    11521 ggttggatcc tcttgaaata gaaaatctag cagatcttcc cagtgcctga gtactatcca
    11581 agggttctgg aattatggcc aatagcaaac ggtgtacata ctagctatgg tcgactttta
    11641 atagtgattt acaggagtca aacaagtccc gtacgctttc ccccaatgcc gcccactttc
    11701 ctttatgtcc ctcacgaatg accatttgaa tgatccgaag tggtgcgccc acaacgtaaa
    11761 acggcgcctg ttcgccggcg gaagcatgac ggcgcataaa caatagccag tgtctcagac
    11821 caatggccgg ttgctataat cgcagacctt aagtctatca attggatttg gatcgcgcct
    11881 gctactccgt tgatatccag acgtctagat caacgaatgt ttttgaccgg cgattgccag
    11941 ataaggtagt agcctttgcg ggaagtgccc gtcaatggcg attgatgtta tctaccatga
    12001 ggcgattcat cttagttctt gttagggggc gctaagatta cgctttcggt ataccctatt
    12061 agtctcgcgc aagttggagc tctgtcagct gagcaagcga cggtaaactg ccgctgtgtt
    12121 ttttactgtc gcactccacg tgaatctagc tgcctgtgcc cttctatgcg cttttgatat
    12181 tactttgttc gctctttcgt cgagcacgtc gagtaatgcg ccggccaaac tacgtgcatc
    12241 gcatggagag acgcatatgg cagctgtgcc tgctatttca ggaagtgatc cggcgtttgc
    12301 gtataccact agagttccac atgccattgc ctccaataca ggaaagccaa aaccctcata
    12361 gagggaaggc atcacacata cctcggccaa gttgtagagt gaaaccaagt cgtcctctga
    12421 aacttcttcg acaaaatgga ttgctgttcg aacgccgagc tggtccgcta acttaatcaa
    12481 gtgctcccgc tcgcgctcaa agtgtgcgcg accaaccttg attagctcta catccggtaa
    12541 t
//
LOCUS       DASUVS010000109        44771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_909535, whole genome shotgun sequence.
ACCESSION   DASUVS010000109 DASUVS010000000
VERSION     DASUVS010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 44771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44771
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909535"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="VF182_06015"
     CDS             <1..1082
                     /locus_tag="VF182_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436665.1"
                     /translation="ESVVLVLRRSMCAFLPFVVVGISILLLAIWVGYSFTDLIFFIFP
                     TDIIADHQAVGRIGSRARGVLPVFLIVTAATLYCYLSDRMRADKATISLFLLLLVPAT
                     FARFFPTYNMLYLGMFPVAVFLACVLPCVLAQNQTLCRRLKTTLILFFTIYSLASNYL
                     LFIRTQLADLKDDRLVRLETGPLSGIFVERDVFELFEEVRARLDLQGVANDEAKSFLS
                     SRYVKFIPLMYGWRDSLAGQNLMIQLGKMWSYDDVIKIKGVESENVFDWEGLIYRWRE
                     AAVDQLKQSQTKVIVMSLFNEEVLEEELEPSFDPLREYLRENFSITDIIEPTMTLYRR
                     SNVPEGAVILTRKTQQPDDPQDGAQ"
     gene            1368..2339
                     /locus_tag="VF182_06020"
     CDS             1368..2339
                     /locus_tag="VF182_06020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6436666.1"
                     /translation="MSFLPSSPFILGQTPWVIEIEDTTTLFAPFPRVNGKRVYPRLLG
                     LDGIYDSGFFPCIKALLENESCRGIICHVRSTAESIPILFRNPELAEKVSYIPMGIRV
                     PPSRKSRTDADTVTLLFTNSWHQGSTGFYLRGGLDVLEAYSVLYSEYKNLRLIIRSKL
                     PDDLAGRYRQMIERCNVAVIDRFLPEDEIRALFENSDIYLLPSARLHVVSLLQAMAYG
                     LTVVASDGWGISEYIEHEKNGLIVAGRYGETSWMTPGGMLMEDYRPLLTPTPKVADAL
                     RDNLATLIKDPRRRRQLGETARRDVETKFSLESWNLGLARAFDRALS"
     gene            2686..3129
                     /locus_tag="VF182_06025"
     CDS             2686..3129
                     /locus_tag="VF182_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436667.1"
                     /translation="MITRALSIAELTTRSDVIVLGTVSFTTSDWNLSRTTIETRIDLK
                     VEEIFKGTVDQAKISFYQLGGVVGDTASSLGEAPTFVEGERVAIFLFKNNRERLQVVG
                     FSQGKFLVERRPEGLMAVRRIPGLSKSLDEFPLDTFKRQVQEAIT"
     gene            3816..5480
                     /locus_tag="VF182_06030"
     CDS             3816..5480
                     /locus_tag="VF182_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX6436668.1"
                     /translation="MPGDIVENHEKFSLLGTASRWFEPDSNSQVSMFLNSQGEANAPG
                     GGFEQIRNAYQAWSTVIGTTFRFHDAGFTSAQGFAFDGINAVSFGDPLGQIDPPQGCS
                     GTLAIGGFFRSTNQTGVANGQTFFRIIEGDLVFADGWQGCGFYENFNNLAEVATHELG
                     HVLGLGHSSDTIATMNAFAHFDGRGAALQTDDIAGINFIYPKNISIDQIQYADVNGDG
                     KADTLNFDVFGGAGVWVSLSTGTDSTVPEMWLQHGPSTPDQIQYADVDGDGKADALYF
                     DTFRSNGIWVSLSTGTGFTAPQMWLQHGPSTPDQIQYADVNGDGKADALYFDTQTGRV
                     LVSLSSGAGFATPEIWAQYPPSTPDQIKYFDVDGDGKADALHFDTNAALRVGISTGSG
                     FTTPSIWLQHGPSTSEQLQYVDVNGDGKVDAVYFDTLRSNGVWVSLSTGTGFLGAQLW
                     LQHGPSTPDQIQYADVDGDGRIDAVYFDTLRSRGVWVSRSTGTSFGAGELWLRHGDST
                     PDQIKYIDVNGDGRADASYFDTSRSKSVWVSPSNGAAFGNGLLWLP"
     gene            complement(5588..7111)
                     /locus_tag="VF182_06035"
     CDS             complement(5588..7111)
                     /locus_tag="VF182_06035"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX6436669.1"
                     /translation="MRQIKLRKFLNQRSIVLVLLALSLSGEALAAQLQLAWVDNSGNE
                     DGFKIDRMTGTTGVFVPIATVGANVTSYTDPNLLDGTNYCYRVKAFNSAGDSAFVEGC
                     KTTGTLETFVLSITKTGNGNGTVTSNPMAVNCGTNCNATLTSGATVSLVPTPAAGSTF
                     TGWSGHADCTDGSVTMNTSKSCIATFNKPTIELRAEWLHDGAQLLTNRTKYADINGDG
                     KIDAIYFDTAGSRGIWVSLGTDDGFAPAEMWLQHGDSTPDQIQYADVNGDGTADALYF
                     DTLRSRGVWVSLSEGIGFKPAEMWVQYGESTPNQIQYADVNGDGKADALYFDSGKSNC
                     VRVSFSTGIGFTSPQSWICHGPSTPDQMQYADLNGDGKVDGIYFDTFRSRGVWVSLST
                     GTGFEPVKNWLQHGESTPNQLQYADVNGDGKADALYFDTFRSRGVWVSLSTGTGFTSG
                     QMWLAHGESTPELIQYADLNGDGKADAIYYDVLRSGGVWVSLSKGDSFATAFLWSTE"
     gene            7558..8499
                     /locus_tag="VF182_06040"
     CDS             7558..8499
                     /locus_tag="VF182_06040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6436670.1"
                     /translation="MDPALVSIILFAHAPYARFIPISLGSILKQSYGSLEVFVLGDGS
                     RELEEAVAPFRKDPRCIPTSQGAQPFLQASNELMRQCHGKYIGTWNSDDIYEVNHVKV
                     LVKALEEDATLGGAFNNTEYFSDQPATNGKEKPKLTLGPMVKNGQGKKLASARLSVHD
                     ILKDNFMTGPSSLITKTAFESVGGYDKDIYLNCDLHWFYRLTAYFPVRYVDYIGVRKR
                     IHPLNNTAVNPHYEYGVRELEHIRDHYPDVYSRIGKSAFNKKLGRKYLRLGLYYERKG
                     ELTKARAAFKQAMLLRKWSLRYHWEYIRSTRGAGGNS"
     gene            8680..9054
                     /locus_tag="VF182_06045"
     CDS             8680..9054
                     /locus_tag="VF182_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6436671.1"
                     /translation="MKPIIYKGDIVDKPWGHELIWAHTERYVGKILHINKGESLSYQY
                     HRVKDETVQLLAGVLEMDLEVDTERTTVRFVPGDCLHITPGMKHRMTAVEECDVLEVS
                     TPELDDVIRLEDRYGRAGTSNE"
     gene            9070..10770
                     /gene="gspE"
                     /locus_tag="VF182_06050"
     CDS             9070..10770
                     /gene="gspE"
                     /locus_tag="VF182_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEX6436672.1"
                     /translation="MESYLSLAERLVERRLLSDDELERVVKLQQDQPAPLTRLMVELG
                     FLSEDDLLPVLRDHFNIPLVSLRDIPTVPLPIEFSANMGEFLKTARMVPVKVEGRDLI
                     VATGDPFDIARLHALEIASGLQATPVLAKDKEISARIEALFGSNTTSDNTQGAVGREI
                     EGAVDEEDVAHLRDMASEVPVIRLVNQMLVRALESRASDVHIEPFENQLKVRYRIDGI
                     LHEVEPPPRQLKAAVVSRLKILAQLNIAERRLPQDGRIKIRLAGKDVDLRIATVPTLY
                     GESVVIRLLERGQIFTQLETIGFPSDILDHFNGMILKPHGMILVTGPTGSGKTTTLYG
                     ALQKINDPGKKIITIEDPVEYQLSGVNQIQVKPQIGLTFANGLRSIVRQDPDVIMVGE
                     IRDAETAEIAVQAALTGHLVFSTLHTNDAAGAISRLLEMGVQDYLLSSSLLGVLAQRL
                     VRRLCKTCRKQVSFSNFDSSEPELSLPSGNLPEQVWEAVGCNICSGTGYLGRVGIFEL
                     LPVTSEICKIIVQRADAGTIRSLAVQQGMRLLRDDGWDKVRHGVTTVAEVLRVTREEA
                     "
     gene            10825..12048
                     /locus_tag="VF182_06055"
     CDS             10825..12048
                     /locus_tag="VF182_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6436673.1"
                     /translation="MAFYQYRAADHAGKIVEGIMEAEAEQGVVLRLHEMGCIPLRIGA
                     SAERAAAASQARLPLFSKRKISQQQLLQFTQELGTLLAAGLPLDRSLSILGNLIEGEE
                     FSKVMRTLLEAVRAGKSLAASMGEHSDVFPKIYINMIRAGESGGILEGVLRYLAEYME
                     RSLALKEDLKSALIYPVILASAAGVSLIVLFLYVIPRFAVIFKDVGQAVPWITRVIIG
                     LSSSLTEYGWLIAIALIAAIAAGIFYLRNPETKSELDRVSLRLWLIGDLVRKFETARF
                     SRTLSALLKGGVPLLEALGTVQGIMGNRLMARAINQVQIRVREGKGMARPLAESGFFP
                     PLALNMIAVGEETGKLEAMLGEVAAFYDQEVKRTTKRLTSIIEPVLILGMGLVIGVVV
                     ISMLLAIFSINDLPF"
     gene            12032..12469
                     /gene="gspG"
                     /locus_tag="VF182_06060"
     CDS             12032..12469
                     /gene="gspG"
                     /locus_tag="VF182_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEX6436674.1"
                     /translation="MIYRSKKYLNNARGFTLVELIVVVIIIGLLAGLVLPQFIRQEEK
                     AKLRTTKAQIELFGTALDTFRLDVGRYPTTDEGLQALRERPGGLERWDGPYLRKELPL
                     DPWSKPYAYRSPGEHGPFDIVSYGADGTPGGDGDNRDITSWEK"
     gene            12444..12908
                     /locus_tag="VF182_06065"
     CDS             12444..12908
                     /locus_tag="VF182_06065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX6436675.1"
                     /translation="MILPAGKNSRGFSLLELILVLMVLGLSGLIVLPNVEKGLRDRDL
                     RQSALSLAAAARDLRSQALQKGVPQRLVLNVSESSYRIARNEIHLPSNVKIASVAGGE
                     VLDNGSRQFLFFPNGSTFGGRIDLSNGADSTSYSIRLHPLTGTIDVMRGGNS"
     gene            12905..13318
                     /locus_tag="VF182_06070"
     CDS             12905..13318
                     /locus_tag="VF182_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX6436676.1"
                     /translation="MRHTTTGCASRRGFTLLEVVVAMAIVGLGVVTLLEIFSSGLQLG
                     ARSRDRTEAVAYGRQVMDQFLARRTVAEGTEQGQIGESSRWQLQVQPVRSVEGLTLGK
                     DWELKDIALEIFVPESGRERRVELRTLRLVKKEDR"
     gene            13318..13956
                     /locus_tag="VF182_06075"
     CDS             13318..13956
                     /locus_tag="VF182_06075"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HEX6436677.1"
                     /translation="MRAPRLGRNSCGFTLIEVVLALSIFALIGAVLYGAFSLSHSAVE
                     KSQASFADNQKLRSFTDLLGSYVRSAYPYRESAQQASVFFDGQEDQLTFVSSISLAMG
                     GRGMSKIRVSWDGAEQGGGPIQLEEELPVRLNVNESQDDGGLRNEVVLQERVKELRFA
                     YLDPKSEDERWEERWDGREKMALPRAVRLTYRDRDGRAIRWIFPVMISVLAQ"
     gene            13962..14978
                     /locus_tag="VF182_06080"
     CDS             13962..14978
                     /locus_tag="VF182_06080"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436678.1"
                     /translation="MEFLQRKLAGVSSERGVALLIVLWIFIFLFVVAFDFSAGVREEA
                     TAAHRYSDDNQGYYLALAGFEHGLYEFLDQARGRELQPVDKPKDIFDAGWREATLGSG
                     TYRVRLVDESSKINLNRADENLLRRIFSNLGIEESLIGVLIDSIMDWRDQDDLHRSNG
                     AEDDYYLSLTPSYTAKNGPFDSLEELLWVKGVSASLFYGYESSGSRDRDSPRVGLREI
                     FSVDSPIDRVNLRTASAPVIHVLLGISLEKCQAFVEERAKLGDKTLADLLPLLGIGSS
                     EAALQMFIFTSPSIVTVEAEGYASESRQPRRVKGVIRAGGGNRGYELTRWVDREIGYP
                     TDGQ"
     gene            15042..16592
                     /locus_tag="VF182_06085"
     CDS             15042..16592
                     /locus_tag="VF182_06085"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX6436679.1"
                     /translation="MNLSNSIRNLPRADFLRSVGIYIMRDHLVLVRLRKNFLNVTLLE
                     QELRDLPIGENRQAISELTGWIAEDVREIALKAEYDSRERALRQAILSLLPHFSAGRD
                     SLYICVPQDQAIIQTVYLPQAAEANLQQVLEYEIERQIPFNRDEIYYDYLPIGKRGDK
                     IGVHLFAVPKKHLAGVLDVLGSFGIVPSGVETTGTALANYLLFCKKDFTGCAAIVGGH
                     ADERELIGVRAGAAGWTQAPELLFSHRLPEAEWAKGIGKELITECLRQTPELYGWGNP
                     DQVVGSVDGEKAQCEDLCALGIEKLGGSSQLTATTLPAVGAALRGVREARLETNFLRP
                     PGAERGARKNLSLLNSVMIGLLILLLVGWGISYPIKDELRLKQLQQENHKNEPYVEAL
                     RREEVQLQTVRKEVQFLSDIDRRKGEVLRVLDELSKVVPTSAYLSNFRYRNGVLEVQG
                     NAENASTLIPLLEKSPLFENVAFNAPSNRGRDNRETFSLKADLEKQATSGPKLPPSEK
                     SKEKAGKL"
     gene            16589..17242
                     /gene="gspM"
                     /locus_tag="VF182_06090"
     CDS             16589..17242
                     /gene="gspM"
                     /locus_tag="VF182_06090"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEX6436680.1"
                     /translation="MKQFWGRLSRRERHLVITTAVILLLVLGRLSVVGPYMAHREWVQ
                     TQLEIQPQLLEKNLRYIGQKEQLTAALEAARNDVKVHETKLLTGDTPSVNASDLQETV
                     QALAAKEGTQVITTRVLNPEATGAFSKIAIQMEVGGQVDQVASLVRGIETSPKLLVID
                     EINIRSLFRPVGLPQQQGAPQAAAQNLRVSITVAGFARGQATGAKSDAPKTTLEKGV"
     gene            17335..18033
                     /locus_tag="VF182_06095"
     CDS             17335..18033
                     /locus_tag="VF182_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436681.1"
                     /translation="MTSAWALCFANNYPEDFEILKRSSLLSILLLVATGLVGWRIYMV
                     WREEPWSLPQPKTMAPAALQDEPATVVPPLPTVTPDLIISKNLFDPERGAGATREAEF
                     NSRSFQRIRNMVLLGTVILGDSRSAIIQDPGSQVSTPNGSAASAGPMRVTLGDSVEGF
                     RLTEIGDRRVVFTKGSSRVEVVLDYFRRDDPAPARAATAPVQRGAPAVAPRVVPNLPR
                     RGALPAPPNPNPKS"
     gene            18049..21249
                     /gene="gspD"
                     /locus_tag="VF182_06100"
     CDS             18049..21249
                     /gene="gspD"
                     /locus_tag="VF182_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEX6436682.1"
                     /translation="MPGKCFRGLVTLVYVTGSVLPLAAQQGTAEPPLTPVPVQPAPTE
                     PNQTQPGQGQPSPSQLPRRAPIVPQRPPSSAQQVPGTPGPSDGSVPQGIPTPAPTPGP
                     TPIPPERRMLPPPSTAGGMVSLNFNRADLVEIIHILAQHLRLTYTIDPEVKGIVTIHS
                     AEPLRPEDLLPIFHQVLRMNNAVAVRTGNIYRITTIKDAKGLVRPVAQGREDSFALQI
                     VPVRFFSAAEMKKLLTPFLTPGGELVDYPRGNFLVIIDLPSNIQRLVEIADMIDVQVF
                     AGTRMEIYQPKVASAEELAAEMTKVMQYYAASAPQQENFASQFVPLPRINQLLVISHS
                     EAAWTYARRWLERVDVAGEGPGRRIFIYPVENGKAIELADVLTQALGQPTTGRRESSR
                     TLQDLHRSTSGGFGQFDSGTRSSSSPFGRSGFQQQNQPLGGAYATVPVPGQAPPAAPA
                     PGVAPGLPSVPGAPRPPAPAAPGAPGAKPEEQLRIVPDPGTNSLIIYGTVQEFQNIKN
                     ILKELDIIPRQVLMDVMVAEVSLTGSEAFGVEYELFRQHNASIFGQTFDSQGAVRSGI
                     FNQATRPATENSPFAQFLNGISGVAGRGNTIRAFISALATDNRAKILSSPSVLATDNR
                     PARIQVGREIPVLSSQSQSVVGDSPIVNSVQYRNTGVILTIIPQVNSQGLVNLQVKQE
                     VSDVGAPSFGNTNSPSFTTRDAETTAVVQDGDTLAIGGIISDTTRRDRSGVPYLMDLP
                     VLGRLFGTTNDTTDRTELVMLITPHVVRNRDEAQQITEGFKEKLYGVRNELERFELEQ
                     ERLKSRRQQSPMPPADIPAVPGAGNKIPNPAPNTSSIPRDLTPSIPLQGLPQHTPQNP
                     APPQPSISLNKRGRKNAPPVDTASTAVTFNRPAEVAVVTELTNRNPPLPWPPLVPSSP
                     TEWVAPADPMQVAQVPQAESIGSALSVRSIDPVAQFEPAPTKASIGNPVPAMDRVITI
                     AKKIDRATPAAEMSHIWMVQVASLPQEKDADQMAATLRQKGYDARVIKAEVDNRLRYR
                     VRVGQLATRNEAVDLNNALKANEKFADSYISRVTVR"
     gene            21453..22268
                     /gene="yqeB"
                     /locus_tag="VF182_06105"
     CDS             21453..22268
                     /gene="yqeB"
                     /locus_tag="VF182_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HEX6436683.1"
                     /translation="MTVSISELRVLIRGGGEMATGIAHRLHRSHMRVLITEIASPTSV
                     RRNVAFAEAVYEGAQTVEGVKAVRVDSYDQAYVAWEQNQIPIFIDPEASIGKILKPVV
                     IVDAIMAKKRGVAKLTDAPLVIGVGPGFTAGLNVHAVVESNRGHHLGRVIWDGAAESD
                     TGIPASVDGYTASRVLRVPRSGIFSALRAIGDSITEGEPVATVNGALIKAEISGQIRG
                     LLRNGIHVEKGIKAGDIDPRGERGYCDSISDKSRAIAGGVLEAVLHSFRDLNS"
     gene            22307..23227
                     /locus_tag="VF182_06110"
     CDS             22307..23227
                     /locus_tag="VF182_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX6436684.1"
                     /translation="MNPKQLTIDDVQEILLKEEYVCDRSLATVVYLSLAMGKPLLLEG
                     EAGVGKTEIAKVLAKVLNAKLIRLQCYEGLDANTALYEWNYPKQMLRIKLEEVQRGDK
                     KAVETEIFNQDYLLKRPLLEAIISEEENPPVLLIDEIDRADVEFEAFLLEVLSDFQIT
                     IPEIGTVQAKQRPYVFLTSNRTREIHDALKRRCLYHWIEYPTFEKELEIVSTKFPEVA
                     PAMAKQICAFMQRVREMNFYKRPGVAETLDWATALIALNRKGLDDKTVVETMGCVFKY
                     REDLHHLKEQIESKHLNLDALLRSSPELMS"
     gene            23233..24444
                     /locus_tag="VF182_06115"
     CDS             23233..24444
                     /locus_tag="VF182_06115"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6436685.1"
                     /translation="MEITPESVAAAVNNFAGVRGHGTVANLLAFGRALKQLGVKISLS
                     QVIDASRSLDLVDIAEKEDFRALLRANLISQVEDFPIFDTVFDYFWREQNYERVPMET
                     LEIEGTPTESQAQEGGEEEGLDEAFAETPAKENVELENLDEFSIPTYSAQELLNRKDF
                     SEMSVEESRAIARAILLIATRIATQVSRRKKRARKGATVDPRWTLRKNIKYGGDVVEL
                     VNRKRRIKKTKVVLLCDVSGSMDCYSRFLIQFMYGLQNELWGVETFVFSTSLTRITHL
                     IRTKDIANALEQISGSILGWSGGTNIGRSLQTFNRNFAPAMVTSRTIVVIISDGWDRG
                     DVSLLERQMQDLKRRCKKVIWLNPLLASENYEPLCKGMQAALPYIDLFLSVHNLNSLV
                     ALGRTLQKMVA"
     gene            24463..24858
                     /locus_tag="VF182_06120"
     CDS             24463..24858
                     /locus_tag="VF182_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX6436686.1"
                     /translation="MSGQGTCFENPELEAKKTDSVYHQVKEFLDKGETVAVATIVSAK
                     GSTPREVGAKMVVTAWGEILGTIGGGCGEADVKREAIEVIRTRKPRLVRVDLLDDISS
                     DSPAVCGGIMNIFVDPWWEGRDEEPAASE"
     gene            24855..25625
                     /locus_tag="VF182_06125"
     CDS             24855..25625
                     /locus_tag="VF182_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEX6436687.1"
                     /translation="MSALSEELVRISEQGLSAVLATIVEAQGSDRVQAGAKCLVVDGE
                     IKTDTIGDPRAVEKIVRESEAILSSEKSKLVALETPDGGRLQVFFEVMLTPPKLIVVG
                     AGHIAVPLVQIAKVLDFHVTVIDDRLLYANRERFPAADEVLVGDMAQMLTEMTITPSF
                     YIVLITRGHKYDEPCLRQILHSKAKYIGMIGSRRRIKACFQRFRDEEKIAEEVIERVY
                     APIGLHIATETPAEIALSILAEVIKVRRGGKAASLSGH"
     gene            25651..26064
                     /locus_tag="VF182_06130"
     CDS             25651..26064
                     /locus_tag="VF182_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF120 domain-containing protein"
                     /protein_id="HEX6436688.1"
                     /translation="MPRKIQGVIFSDLGQASKFMALDWVQDALRKALGFAPYPATLNL
                     RPSTPQDARQWQAIQREVAPEPMAPIESGFCNAGLYSVSIQRLIRGDETVKGAVLLPA
                     VIDYPEDKIEIVAPVRLKQALSVEDGDSLVLEFND"
     gene            26061..26714
                     /locus_tag="VF182_06135"
     CDS             26061..26714
                     /locus_tag="VF182_06135"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX6436689.1"
                     /translation="MNNKKRVIVGISGATGAIYGVRILEILAKLADVETHLVLSKAGR
                     MTIQVETPYSSKDVEAMADVVHDVNNVGASISSGSFRTEGMVIAPCSMKSMGGIAHSL
                     GGDLLVRAADVVLKERKKLVLVVRETPLHLGHLEAMVSLTRMGAIIFPPVPAFYHRPK
                     TLDDVINQTVTRILDQFGIDVKLFHRWDDEGMSRHPEAGKTTTLAAAKTSQRVKKQS"
     gene            26733..27782
                     /gene="fni"
                     /locus_tag="VF182_06140"
     CDS             26733..27782
                     /gene="fni"
                     /locus_tag="VF182_06140"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEX6436690.1"
                     /translation="MPSRENKTLTKTQQRKKEHLELCLDAKTVSAPTGSGLDSYMFLH
                     NALPDLDLDEIDTETTFLGKRLKAPLVISSMTGGFSLARTVNRNLAAAAQELGLGMGV
                     GSQRVAIEEPSAADSFQVRDVAPDILLLANLGAVQLNYGYGVDQCRRAVEMIGADALI
                     LHLNVLQEAVQPEGNRNFSGLTAKIAAVCRDLEVPVIAKEVGSGISAEVALRLARAGV
                     KAIDVAGKGGTSWYAVEASRAAKAGQPVDVSFADWGIPTDQSLLAIREVLPEMTLVAS
                     GGIRSGLDMAKAIALGADLAALGQPLLAPALESAEKVVASLRSIIYEIKVAMLCVGAH
                     DLGALRRTPLLQRPL"
     gene            27843..28322
                     /locus_tag="VF182_06145"
     CDS             27843..28322
                     /locus_tag="VF182_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6436691.1"
                     /translation="MPDDLKQQLIQYLQTHNTMTLATCADERPWAATVFYASEEWKLY
                     FFSAPQSRHCQNLAANAGVAVTIQEDYKDWRRIKGIQLEGKGLPVDSILEKTKAMAVY
                     ARKYPEIIKLFTTPGSGVFYQAFLKVKFYCVVPERIYFIDNEQGFGKRQELELTGQV"
     gene            28335..28805
                     /locus_tag="VF182_06150"
     CDS             28335..28805
                     /locus_tag="VF182_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6436692.1"
                     /translation="MSQVLGNELTVELLSRLDGRDVESHEGKIIPIVTVDEYGWPHPA
                     LLSYYEMVAKNSRTIDMALWKNSSTANNLRQTGKVTLMIIDHALNYYLKGTVSQTEAE
                     MSGAAAVSRFRVTLEQVIEDQEPNAEITTGLTYRRMTRRDPNDFAAKVFRGLKA"
     gene            28816..30114
                     /locus_tag="VF182_06155"
     CDS             28816..30114
                     /locus_tag="VF182_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX6436693.1"
                     /translation="MNRKKAIAWLAAASVIGLGGVGWWQLGDGSKEPPYVTTPAQRAN
                     ITQVVSSTGTLQAVVTVQVGSQVSGTIEKLFADFNTKVKAGQIVAQLNQDKFSAAEDQ
                     ARANLLAAQSNIEKSKVSVADALRTLERNRQLRTKELMAQSDLDAAQAAYDAAVAQVE
                     VNRAQAAQAQASLKQATVDLNNTVIRSPVDGLVISRNVDVGQTVAASLQAPTLFTIAN
                     ELARMEVHTNVDEADVGNVREEQEVTFTVDAFPSRRFRGRVHQVRNAPIVVQNVVTYV
                     AVVRIDNKELLLKPGMTANVQFLVNRKEDVLTIPNMALRFKPPEEKEEAQELLRREQS
                     RTAAKVGERRTSRQSVAGAGAAAVRGTRRVRIYVLRGDKAQPIEIQLGITDGSKTEVA
                     SADLKENDPVIIGMSSGATSGNPGVANPFQPPPTRGFGFR"
     gene            30111..30824
                     /locus_tag="VF182_06160"
     CDS             30111..30824
                     /locus_tag="VF182_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6436694.1"
                     /translation="MSELIRVKELCKTYRMGDVEVPALRGVTLAIRQGEFVAVMGSSG
                     SGKSTFMNILGCLDRPTSGKYFLDGDEVGALTPDQWADIRNRRIGFVFQGFNLLARTT
                     ALENVELPMMYNGCSGKQRRQRAQEVLGLVGLDERLDHMPNQLSGGQQQRVAIARALV
                     NRPSLILADEPTGNLDSATSGEIMGFFRTLNRDQGITIVLVTHETDIAEYAQRQIHFR
                     DGSVVSDHGTESPHLSIEH"
     gene            30828..32042
                     /locus_tag="VF182_06165"
     CDS             30828..32042
                     /locus_tag="VF182_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6436695.1"
                     /translation="MLFMTLRIALRALGRNKLRAFLTMLGIIIGVGAVIAMVAIGEGA
                     RVTIRSQIASLGTNVLIVLPGTTAQGGVRTGSGGVNTLVDGDAKAMMRELPAVASASP
                     ALRRPEQVVAGNLNWSTLTQGVAPEFQQIRDWQVAEGRFIHDGDVESAAKVAVIGETV
                     LNQLFGNDDPIDAVIRIRNIPFRVVGVLAPKGQTSQGTDQDDTIIIPYTTMQKRLMRI
                     TFVQSIVASAVNAERLPEAQAQITALLRQRHRIGPDRQDDFYIRNLSDIAEAASSSAR
                     VMAVLLGSVASISLLVGGIGIMNIMLVSVTERTREIGIRMAVGARRKDIMLQFLVEAV
                     VMAATGGSIGILLGIGSSEILNDWVQWPTHISASIIALAFFFSAAVGIFFGFYPARKA
                     ATLDPIDALRYE"
     gene            complement(32033..34189)
                     /gene="rnr"
                     /locus_tag="VF182_06170"
     CDS             complement(32033..34189)
                     /gene="rnr"
                     /locus_tag="VF182_06170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX6436696.1"
                     /translation="MARKSATATTESPQAREKICDLFKGKPRLRLTPVEIARKGGFKQ
                     HDLQIVIDSLRRLVREGRMVRLKKNHYGLPDSDNLMTGRVHAHPDGYGFLILDDKSAV
                     DLYLNRREMRRVMHGDRVMVRVDRNKRGGLETHIMQVLERGHKRLIGTYDEIEGHGYL
                     VPMDPRIAPVIPLTRGHERPEKGQIIATEISRYGTATSPPQAELLKVIGNPDDPEVQV
                     QSIVFRYGLSSSFPPEVHRETSSASFSVSPDEITSRTDLRHLPIVTIDGERARDFDDA
                     VYVRKTDSGYELYVCIADVAHYVSTATALDQEAYSRATSVYFPDRAIPMLPEALSNGI
                     CSLNPREDRLTKTACIEYSFNGEVIGSRFFNSVIRSRERMTYTDVGRILVDRDRPTIE
                     RYTDLVSQFELMEELTLLIFEKRKARGNLDFDLPEAEIILDIQGVPEDIIRAERNIAH
                     RIIEEFMIAANEAVARHLTARQLPALYRVHEGPEKESLEAVAPFLLSLGYRLPLKKDK
                     IAPLEIQKLLEAARGKPEERVLNRVLLRAMKQALYQPENIGHFGLASRCYTHFTSPIR
                     RYPDLIVHRMLEKALRDEKVTSAERENLSAYLVEAGKHTSDKERNAMDAEREMVALKK
                     TQFMMDKIGEEYNGFITSLTNFGFFVELDSYFVEGLVKIGSLTDDDYYYYEKEYLIKG
                     LRHGRKFRLGDNVRVKVARINAFRSEIDFELVSLHS"
     gene            complement(34245..34520)
                     /locus_tag="VF182_06175"
     CDS             complement(34245..34520)
                     /locus_tag="VF182_06175"
                     /inference="COORDINATES: protein motif:HMM:NF027581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HEX6436697.1"
                     /translation="MSLVPIYLEICPRDIAYVKFVLESYEEVGIIRTIDRKKAMIVLL
                     AVADFIEVARTIIKSLQQEITLTEIPPPPDLGDDWLMTELAIDPSRT"
     gene            complement(34524..37181)
                     /locus_tag="VF182_06180"
     CDS             complement(34524..37181)
                     /locus_tag="VF182_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX6436698.1"
                     /translation="MSKKSISFAVGTVIIAALALVYSVNLLAAIRRDLVYEELQRVAG
                     KDVAFDEFEMTLLTGLGFSAKNFRIGDRPIFAATPLLRAQELRMGVSLLSLMFGRIVI
                     NKLTFVEPEFQIITNEEGLMNISSLEDRRKQLVETPNARSSASKRQHSSINFVVTNFQ
                     IKNGRIDFIDRSVRAPAEMQIKRVDIDLQGINPNGRAKMSLAASLSEDLDQDVRVAGY
                     LGPFVRGKGWKEQPVEVNVEFDSLFVPLLTRAVPFFRDKIPPELDIAGPLSLRAKLAG
                     VLARPQIVDFIVKAPILGSSDYNAVLNGSADLSTNGLWSEAQLKGNLRLSSIDITRLQ
                     GTRVFQDVLPIELSGEGEVSFDSVIEGTWDKLRLGAIVRARNSEIRYGTWLRKPRGTI
                     AEWSSKISRQDNRFVIHESLLTVGSTKINVSGVYERGSRPRLQLRARSDRSDLSEVIE
                     LFPALQAFRTTGNLHGEIVASKVMIGESSWNMNGALEINEAELRHESSRRKIERLRAK
                     ILFLGKEARIENAAFRVRSSDIEMIGAVADLAVPALTCELRSANLHLVDLAEFPVAKS
                     DRLQNVRLRGKLTLENGTPWLRGTVSSPDGTLQDLPYLDLRSQIVWSAEEISLREFSF
                     RALNGSWRSRDVSITGTDKSQRLELYAQLNSVDLPSLLISKFPSFKDRIEGHLNFQGE
                     FSAEAHNGAMKPGSLEGAGETRVPGGTLKDFNLIALMMSRIGSSGSAISLPLSPSLAE
                     LGRSKDTPFENLEALVILDQEVIRTENLQLTTADYKIMAAGWMMTDQATRWNGMLVMS
                     VRISQELLRENKSLRYLLDRSGQINLPFRVEGTPANLRVRPDTRIIAQIIRRGSLPKT
                     IEPPAIDKRSDAQEQKESLPAELEQFLSR"
     gene            complement(37294..38496)
                     /locus_tag="VF182_06185"
     CDS             complement(37294..38496)
                     /locus_tag="VF182_06185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX6436699.1"
                     /translation="MKEAIIVEAVRTPMGRHSGILKDIRTDDLGAYIIAKLVERTGVN
                     KEEIEDVYFGCTNQAGEDSRNVARNASLLAGLPYSIPGATVNRLCGSGLEAINQAGRA
                     VETDHGDLFVAGGVESMTRGPWVMGKPNTSFQRGDVTMYDSSLGWRFPNKRMGELYSL
                     INNGETAENVADKYHISREDQDAFALSSHKKALQAQDRGVLRDEIIAVEIPQRKGAPL
                     VHDKDEGPRADTSLEKLAALQPAFRKGGSVTAGNSSPLSDGAAALLIATPEKAEQLRL
                     KPMARIIASAAAGVHPNYMGIGPIPAAQKAMKRAGLTIDQIDLVEINEAFASQVLACA
                     RELGIDLSKLNVNGGAIALGHPLGCSGARIMTTLVHEMKRRGSRYGLATMCIGVGQGI
                     ATIVERVN"
     gene            complement(38493..39242)
                     /locus_tag="VF182_06190"
     CDS             complement(38493..39242)
                     /locus_tag="VF182_06190"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6436700.1"
                     /translation="MSTTKSSLAIVGTNRLAQDWIALSKAKGLDAELVSDAGQIQADA
                     ALVIDTSAGDEAMKRRTLEKLDRGLTPSAVIITSCLVGSTTLMASWTKRPERLIGFAT
                     FYPLADRKIIEIAAGLRTSDSALQQAQDHFRSLGKEFVKVKDAAGLTFPRILSLIINE
                     AARSLEEGVAEADEIDIAMRLGVNYPMGPLRWADQIGLDEVLAVLEGLERETGDDRYR
                     PAPLIKKLVMAGFHGETTGRGFYSYEEQVKS"
     gene            complement(39223..40089)
                     /locus_tag="VF182_06195"
     CDS             complement(39223..40089)
                     /locus_tag="VF182_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016741463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX6436701.1"
                     /translation="MTNPIEKIAVLGAGTMGAGIAQVAAQAGFDTLLYDIKQEFIDTG
                     LGRIRGFLKGSRERGKLSVEQEQEIVARLRSTTQLEACQGSELVIEAAPEKLELKRDI
                     FKQLDSICGRETLLASNTSSFNITAIAANTQHQERVLGLHFFNPPPLMALVEVVQGDR
                     TSPASIDKATDLMRRMGKTPARAKDTPGFIVNRIARPFYNEALRILGDGDGSVEIIDR
                     VMKEAGNFRMGPFELMDLIGNDINFAATDSLYQSFFHDPRFRPSPIQQRMVMGGNLGR
                     KTGRGFYVYDKK"
     gene            complement(40140..40919)
                     /locus_tag="VF182_06200"
     CDS             complement(40140..40919)
                     /locus_tag="VF182_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6436702.1"
                     /translation="MAYETIIYKKESGVATITLNRPQALNAFVPQMNQEVMDALKVGE
                     RDQEVRCFMITGAGRAFCAGQDLKGRTPDQKGSLGASLREKYNPMIRQIRQMEKIVIA
                     AVNGVAAGAGCNFALACDLRVASEDAKFIQSFVRVGLAPDSGGSFILPRLVGLSKAME
                     LLLLGETVDAREAQRIGLVAKVFPSAEFSDAARKLAERVAGAPRGIGLIKRAVNHANL
                     ASLEAALEYEACLQEIAGRSSDYDEGVRAFLEKRAPSFTGK"
     gene            complement(40935..41708)
                     /locus_tag="VF182_06205"
     CDS             complement(40935..41708)
                     /locus_tag="VF182_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6436703.1"
                     /translation="MDYENVVVEIDGAIAVVSLNRPQQLNSLSYDLVKDLSLAMQALD
                     QDQGIRVIIVTGGEKVFAAGADIKEMADLGAFDERVQGRLAYRDKINQISKPVIAAVS
                     GFALGGGCELAMSCDIIVASETARFGQPEVNLAIIPGSGGTQRLTHLIGKHRAMELVL
                     TGEIIGAAEAERLGLVNRVVPVELMLEEAKNIAKKIAVKPKLAIQAAKEAVLKAANTA
                     LDEGLDFERKSFYMLLASEDRKEGMKAFLEKRKAEYKGR"
     gene            41972..42652
                     /locus_tag="VF182_06210"
     CDS             41972..42652
                     /locus_tag="VF182_06210"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6436704.1"
                     /translation="MPSRLEPATRHYDEKLQHILKTSAKIFAEKGFHRTSVRDISRAT
                     KMSLAGLYYYFTTKEELLYLIQERCFVTLLHRWEQAAGAEMDVRARIGLFVANHLSFF
                     WHNTHEMKVMAHEDESLNGDFADKILVLKRRYVKILMDLVEEWQQQDGGKGVDLRVAT
                     FSLFGMMNWTYTWYQAKRDLPLPQLIEQMVRIFFFGVLKAGQIDERWFISNTSREKNA
                     FSLWHNVP"
     gene            42677..43210
                     /locus_tag="VF182_06215"
     CDS             42677..43210
                     /locus_tag="VF182_06215"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX6436705.1"
                     /translation="MSLARVGDKAPDFAVKGMHQKRVSEYSLSRFKGKWLVLFFYPAD
                     FTFICPTEVVGFSKLAKEFAAQDAEILGVSVDSLDSHRSWAEELGGIDYPLLSDEDKS
                     LSRAYGVLDEREGVPLRATFIINPNGEVSYLVISHVNVGRSVEETLRVVKALRTERLC
                     PSGWKPGEPTGGLDHKY"
     gene            43299..44030
                     /locus_tag="VF182_06220"
     CDS             43299..44030
                     /locus_tag="VF182_06220"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX6436706.1"
                     /translation="MALEVIQQKKRFIESRARIREFVFGIQDGLISTVGLLAGIQGAT
                     ENRLVVIIAGFTAMFSGAISMAAGSYLSSAAQKEIFDKELRDAEALAEREPYVAAEGL
                     LRALGEEGLSKEQSYRMVRVLSQERNVFMRTFQEKVFGLGSAEINQPVQAALVMGFSF
                     IVGAIVPIIPYMITSGLTAFYLSALLGGITLFGVGAFKAKLAGKSLALGGLEFFLVAV
                     GAAGAGFLIGLVVQYFFPGVDIPPA"
     gene            complement(44169..>44771)
                     /locus_tag="VF182_06225"
     CDS             complement(44169..>44771)
                     /locus_tag="VF182_06225"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX6436707.1"
                     /translation="PGSHVGAGEDQGIKTIASSLNEIHQACPGYRVKVTLEITAGQGS
                     NLGFRFEHIVKMFDATRESDRLRVCFDTEHAFAAGYDIRSKEGYERTFTEFDETIGIE
                     RLAAFHLNDSKKEFNSRVDRHEHIGKGHIGLEAFRLLMNDKRFWGVPMCLETPKGLDL
                     AEDRENLGLLRSLFMPSSVVQTDTALLASASERRQPSGPV"
BASE COUNT        10164 a        11603 c        12443 g        10561 t
ORIGIN      
        1 aagaatctgt ggtgttggtc ttgcgcagat cgatgtgcgc attcctgccg ttcgtggtcg
       61 taggcatttc gattcttctt ctggcgattt gggtcggtta ttctttcact gatctgatct
      121 tttttatctt tcccacagat atcatcgcag accaccaagc agtcggaaga attggctcgc
      181 gagcaagggg agtgcttccg gtctttttga ttgttaccgc agcgacattg tactgttacc
      241 tcagcgatcg aatgcgggca gacaaagcca cgatcagtct gttcctgctg ttgttagttc
      301 cagcgacttt cgcccgattc tttccaactt ataacatgct ctacctcgga atgtttcctg
      361 tagcggtgtt cctagcgtgt gtattgccgt gcgttctagc acagaaccag acattatgcc
      421 ggcggctcaa gacgacccta atactttttt ttactattta ctctttggca tcgaactatc
      481 ttttgttcat aaggacacag ctcgcagacc tcaaggatga tcgtttagta agactcgaaa
      541 ccgggcctct atccggaatt tttgtcgaaa gagacgtatt tgagctcttt gaggaggttc
      601 gagcacggtt ggatttgcag ggcgttgcca acgacgaggc aaaaagtttt ctctcctccc
      661 gttacgtgaa gtttattcca ctaatgtatg gctggagaga ttctctggcc ggtcagaatc
      721 taatgattca actaggaaaa atgtggtctt acgacgacgt catcaaaatc aagggggtgg
      781 agtcagagaa cgtttttgac tgggagggtt tgatttatcg ctggcgcgaa gcggcggtgg
      841 atcaactgaa acagtcccaa acaaaagtaa tagtgatgag cctgtttaac gaagaagtcc
      901 ttgaagaaga gttggagcct tccttcgacc cattgagaga atacttacgt gaaaatttta
      961 gcatcaccga catcattgaa ccgaccatga ctctttacag aagatcgaat gttccggagg
     1021 gagcagtcat tctaacacgc aagactcagc agccggatga tccgcaggat ggggcacaat
     1081 aagggcctgg ttgcttgtgc atagcaatga ggaaagtttt cttcgctggt catctccggc
     1141 cattcaagca cgttgggcgg catcctcaat tcgagcatat ccataatcca ccggctggtt
     1201 atgagtttgt aactagcagc cagtctagct ctccgaaatc ccgcacgtta aaatcgctga
     1261 tgcggttttg ccgtgacgct ctagcaaacg ggtcgacagt taaggacttg tcgagattta
     1321 ttcggtcccg ttcgcttcga tcccaaatct cctttccgga aagcgttttg tcatttcttc
     1381 cgagctcgcc tttcattctc gggcaaaccc cgtgggtgat cgagattgag gacaccacga
     1441 cgttgtttgc gccttttcca agagtcaacg gcaagcgagt ctatccgcga ttattaggat
     1501 tggatgggat ctacgactcc ggcttctttc catgcataaa agccttgctg gaaaatgaaa
     1561 gctgtcgagg tattatctgt cacgttcgct ctactgccga gagcattccg attttgttta
     1621 gaaatccgga actcgcagaa aaagtcagct atattccgat gggtattcgg gtcccgccgt
     1681 ctcgaaaaag cagaaccgac gccgatacgg ttacgctatt gtttactaat tcctggcatc
     1741 aaggttccac cggcttttac ctgcgcggcg gtctcgatgt cttggaagct tactcagtgc
     1801 tttattccga atacaaaaat ttgcgactta tcatcagaag caagctgccc gacgacttgg
     1861 ctggccgata tcgtcaaatg atcgaacgct gcaatgttgc ggtaatcgat cgatttcttc
     1921 cggaagacga aattcgtgcg ctgttcgaaa attcggatat ctacttattg ccctccgccc
     1981 ggctgcacgt ggtctcgctt ttacaagcga tggcttacgg tttgacagtt gtggcctcgg
     2041 acggctgggg gatttcagag tacattgaac atgagaaaaa cggcttgata gtcgccggtc
     2101 gctatggcga gacctcgtgg atgacccctg gcggtatgct tatggaagat tacaggccgc
     2161 tcttaacgcc gacgcctaag gtagcggatg ccttaaggga taaccttgct acgctcatca
     2221 aggacccacg caggcgacga cagttgggcg agaccgccag gagagatgtt gagacaaagt
     2281 tttcgcttga gtcatggaac ttgggtctgg cgcgagcatt cgaccgcgct ttatcgtgag
     2341 cctcaactgc gcgccgcatg cgtaaggccg attactgtcc cgaaaactcc catgtcgggt
     2401 ttagggacag cttttggcag gagacaggga gacaggcgct gccggaagca tcggcacgtg
     2461 tagtcacgtg gatgtcgcat ggcacgtaat actatgaatt aagttgacat ctattccatt
     2521 ttaggcaatt gtgagcgccg caaacggcga ccgacattca tgctctccag tgaaactcaa
     2581 ggaactggca ggcttgccaa actgtcatac gcactcgcga ctcgtcgcac agtatttcta
     2641 ttcatcctaa catttctgtt ggctggcgac agtcgggcga gttctgtgat tactcgcgcg
     2701 ctttcgatcg ctgagctaac gacgcggtcg gatgtcatcg ttcttggcac ggtaagtttc
     2761 acaacgagtg attggaactt aagtcgaaca acaattgaga ctcggattga tttgaaagtg
     2821 gaagaaattt tcaaaggtac agttgaccaa gcgaagatca gcttttacca acttggtggt
     2881 gtggtaggag ataccgctag ttcgctcggc gaagcgccga cttttgtaga gggagaaaga
     2941 gtcgccatat ttttgtttaa aaacaatcga gaaagattac aagtagtggg cttttcccaa
     3001 ggtaagttct tggtagaacg acgccctgaa gggctgatgg cggtgcgaag aattccggga
     3061 cttagtaaat cactggatga attccccctt gacaccttta agcggcaagt tcaagaggca
     3121 atcacatagt actaggtggt ctgacaattt ttaaacaagg atcgatcaac ttgattcatc
     3181 aacagcggac cagagtattt tcgcgccgac gcccgatgct actgatcttt tgcgctattt
     3241 tgatttccgt catgggccat ctgaggaatg ccgatgcaac tattatcata cccactgacg
     3301 atcgtaacct gattcgacaa gcaagtacaa tcgtactagg gaaggttaca agaattgatg
     3361 gccattggta tgcggcaaag acgcaaatct atacccatat cgagattacg atcgaggaag
     3421 tcctcaaagg acaagtcggt gacgctgaga taacgatcaa gcagttaggt ggagtcgttg
     3481 gagggctgca gtcctggatc ttcggaagcc ctgaatttgc gatgggcgaa cgagttctgc
     3541 tatttctgac gccaaattcc gatggcagtc cacgggtgct aaacttatac gcgggaaagt
     3601 tttcgatcgt gcgggattca cgaaccggag aacaatttgc ctccagatat tcgatgccgg
     3661 aaggtgtcca acgtctagcg ccttcaggta caaatccaac ttcagctagt gcgattgtcg
     3721 aaatgcgagc cttgagccgg ttcaaagcgg aaatagcggc tcaagtcgcg caagaacaag
     3781 caaatcggtc ggcggctctt tctattctgc atgcgttgcc gggagacatc gtcgaaaatc
     3841 atgaaaagtt tagtttactt ggcactgcgt caaggtggtt tgagccggac agcaacagcc
     3901 aggtctcgat gttcttgaat tctcagggag aagccaatgc tccaggtggt gggtttgagc
     3961 agatccgtaa tgcttatcag gcttggagca ccgttatagg cacgacattt agatttcatg
     4021 acgctggctt tacctctgcg caagggtttg cgtttgacgg tatcaatgcg gtttcgtttg
     4081 gtgacccact gggtcagatc gatccaccac agggttgcag tggcacacta gctatcggcg
     4141 gattctttcg aagtacgaat cagactggag tggccaatgg tcaaacgttt tttcggatca
     4201 ttgaaggaga tctggtattc gccgacggct ggcagggttg cgggttttat gaaaatttca
     4261 acaatctcgc ggaagtagca acccatgaac taggccacgt gcttggtttg ggccattcgt
     4321 cagatacgat tgcgactatg aatgcttttg cccacttcga cggtcgaggt gccgcgctac
     4381 aaactgacga catcgcaggc ataaacttta tctatcctaa aaacatttct atagatcaaa
     4441 tccaatatgc tgatgtaaac ggagacggta aggccgatac tctcaatttc gacgttttcg
     4501 ggggagcagg ggtttgggtg agtctctcga ccggcaccga ttcaacagtt ccagaaatgt
     4561 ggttgcagca tgggccgtca actcccgacc aaattcaata cgctgacgta gatggtgatg
     4621 gcaaagccga tgcactctac ttcgatactt ttcgaagtaa cgggatctgg gtcagtttgt
     4681 cgacaggcac tggtttcact gcgccgcaaa tgtggcttca gcacggtcca tcaacacccg
     4741 atcaaattca gtatgccgat gtcaatgggg atggcaaagc tgatgcgctg tatttcgata
     4801 ctcaaactgg acgcgtattg gtgagtctat ccagcggagc tggctttgcg acaccggaaa
     4861 tatgggctca atacccgcca tcgacgccgg atcagatcaa gtattttgat gtagacgggg
     4921 acggcaaagc cgatgcgctc cattttgaca ccaacgcagc gctacgggtc ggaatatcga
     4981 ctggctcggg tttcacgact ccgtcgatct ggctgcagca cggaccgtcg acatccgagc
     5041 aacttcaata tgttgatgtt aatggagatg gaaaggtcga tgcagtctat ttcgataccc
     5101 tgaggagcaa tggtgtctgg gtgagtctat caacaggcac cggtttctta ggggcgcaat
     5161 tatggcttca acacgggcct tcgactcctg atcagatcca atatgcggat gtggacggag
     5221 atgggaggat cgatgcagtc tactttgaca cgctgcgcag tagaggagta tgggtaagtc
     5281 ggtcaacggg aacgtcgttc ggtgctggag agttgtggtt gcggcacgga gactctactc
     5341 cggatcagat taaatatatc gatgtgaacg gagacggcag agccgatgca tcctatttcg
     5401 acacttcaag gagtaagagc gtctgggtta gcccgtcaaa cggcgctgct ttcggtaatg
     5461 gccttctttg gctcccgtga gctgtatccg gagctccgac catcttgtga ttctctgaat
     5521 ggaagcggct gcctagagat tacccggagg ttctcgcgtt gccggcgtct ttttcgcgat
     5581 cgattgatca ctctgttgac cagagaaaag ccgtagcaaa actatcaccc ttagataagc
     5641 tcacccacac gcctccgctg cgtagaacgt cgtagtaaat agcgtcggct tttccatctc
     5701 cgttgagatc agcgtattga attagctccg gtgtcgactc tccatgcgcc agccacattt
     5761 gacctgatgt gaaacctgtt cctgttgata agctcaccca aacgcctcgg ctgcgaaagg
     5821 tgtcaaaata gagcgcatct gctttaccgt ctccattgac atcagcgtac tgaagctgat
     5881 tgggcgtcga ctcaccatgt tgcagccagt ttttaaccgg ctcgaacccg gttccagtgg
     5941 ataggctcac ccatacgcct cggctgcgga atgtatcaaa gtatatccca tcaactttcc
     6001 catccccatt cagatctgca tactgcatct gatccggtgt tgatggtcca tgacaaatcc
     6061 aagattgcgg cgaagtaaag ccaatgcccg tcgagaaact gacgcggaca caattcgatt
     6121 taccgctgtc aaagtaaagc gcgtcggctt taccatcccc attgacatct gcgtactgga
     6181 tctggtttgg agtagactca ccgtactgta cccacatctc tgccggttta aatccaatcc
     6241 cttcagacag gctaacccaa acgcctcggc tccgcagcgt gtcgaagtac agcgcgtcgg
     6301 ctgttccgtc tccattgaca tccgcatatt gaatttgatc cggtgtcgaa tctccgtgtt
     6361 gcagccacat ctctgcgggg gcaaacccgt catcggtgcc tagactaacc cagattcctc
     6421 ggcttcctgc cgtatcaaaa taaattgcat cgattttccc gtcgccattg atgtctgcat
     6481 acttggtccg gttggttaat agctgcgcgc catcgtgcag ccactccgcc cgcagctcga
     6541 ttgtgggttt attgaatgtc gcgatgcagc ttttgcttgt gttcatcgta accgaaccat
     6601 ccgtacaatc ggcatgaccg ctccatccgg taaacgttga tcctgcagct ggagtcggaa
     6661 ctaacgaaac ggtcgctcca cttgtcaatg tcgcattaca gtttgtgccg cagttcacgg
     6721 ccatggggtt agatgtgact gttccattgc catttccggt tttagtgatc gacaaaacaa
     6781 aagtctccag cgtcccagta gttttgcatc cctcaacgaa agcagaatct ccggcgctat
     6841 tgaaagcttt gacccgataa caatagttgg ttccatccag aagatttgga tcagtgtagg
     6901 aagttacgtt tgcgccaacc gtcgcaattg ggacgaagac tccagttgtt ccagtcatcc
     6961 gatcaatctt aaaaccatct tcattaccgg aattatctac ccacgccagc tgaagttgag
     7021 ccgcgagcgc ctcaccgctc aatgaaagag ccagtagaac cagcactata gaccgctgat
     7081 ttagaaattt ccggagtttt atttgcctca tagtagagcc tcgtccaccg aagcctacgc
     7141 atgacaaatg ccatgcggcg attgcaagca ttgatcagaa atcggcggat ttgggcattg
     7201 ccttacaaga ttgctccgat ctggcactgt acattcttgg gcgtggcgct gaagcgctcg
     7261 gatttcaaaa tgagcaccag agaagagcat gtagctgaag agagaatgcg gggcttagag
     7321 ttgaacgtcg gagtgctcgc cgcgcaatca tcgattaata ctgctccgcg tgggaagcac
     7381 gaagttagtg ggaaccgcct ggaatctcgc tgtgcaacag aaagagggtt gtacaggaag
     7441 gtgtggttag tctgacccca gaaatttgtt tcgcttggaa tggatcgaga ttgcttgact
     7501 ttcgggaggt tttttccgat acctgctgag gtcgatagct agtacacggt tttatctatg
     7561 gacccagcgc tggtttccat cattctcttt gctcatgcgc catatgcgag atttatcccg
     7621 atctcactcg gatctattct gaagcagtct tacggttcac tggaggtatt cgtgcttggc
     7681 gatggctcta gagaattaga agaggctgtc gcgccattca ggaaagatcc tcgctgcatt
     7741 ccaacttctc agggcgctca gccattcctg caggcgagta atgagctaat gaggcaatgt
     7801 catggaaagt acattggtac ctggaacagt gacgacatct acgaagttaa tcacgtcaag
     7861 gtcttggtca aagcgctcga agaagatgca accctggggg gagcgttcaa taacacggag
     7921 tatttcagcg atcagcccgc tactaacggc aaagagaagc ctaagttgac cttgggcccg
     7981 atggtcaaaa acggccaggg taagaagctc gcttcagcgc ggctttcagt gcacgacatc
     8041 cttaaggata attttatgac cggcccctct tcattgataa caaagacagc attcgaatca
     8101 gtcggaggtt atgacaaaga catttacctt aattgtgatc tgcactggtt ttatcggctc
     8161 accgcctact ttccggtacg atacgtcgac tatatcgggg tgagaaagcg tatccatcca
     8221 ttaaataaca cagctgtgaa ccctcactat gaatatggcg tgcgcgagct cgagcatatc
     8281 cgcgaccatt acccggatgt ctatagccgc attggcaaga gcgctttcaa caaaaaatta
     8341 ggaagaaaat atttgcggct tggtttatac tacgagagaa agggtgaatt gaccaaagcg
     8401 agggcggctt tcaagcaggc catgctcctt agaaagtgga gcttgcgcta ccattgggaa
     8461 tacattcgtt ccactcgagg cgcaggcggt aattcatagg ttgtggcagt cttgaacctc
     8521 tggcattatc tctagcgaac gtttgcgctt cgatccactc tccgcgttcc gacatcttgg
     8581 taatagattg acgttcacgt ttttgcaggg tgccggagaa acgtctctat tgcggcgttt
     8641 tcgctctatt gcgaatctgt tatagtgacg ccctaacaag tgaagcccat aatttacaaa
     8701 ggagacatag tcgacaaacc atggggacac gagctcatat gggcccatac ggagcgctat
     8761 gtcgggaaaa ttcttcatat caacaaaggc gaatctctga gctatcagta tcatcgtgtg
     8821 aaggacgaga ccgttcaact attggccgga gttctcgaaa tggatcttga ggttgacaca
     8881 gaaagaacca ccgtgcgctt cgtccccggc gactgccttc atatcactcc cggtatgaag
     8941 caccgcatga ctgcggtgga ggaatgcgac gtattggaag tctcgacgcc cgagctggac
     9001 gatgtaatcc gtttggagga ccgctatggt cgcgccggca cttcaaatga atagctgagt
     9061 taaagcttta tggaatcgta cctgagtctt gccgaacgtc tagtggaacg acgactgctt
     9121 tccgatgacg aattggagcg cgtagtgaag ctgcaacagg accagccggc tcctttgacg
     9181 cgcctcatgg ttgagcttgg ctttctttcg gaggacgatc tgctgcctgt actccgcgat
     9241 catttcaata tcccgctggt gtctttgcgt gatattccga ccgtgccgct gccgattgaa
     9301 ttttctgcca acatgggaga attccttaaa accgcccgaa tggtcccggt gaaagtcgaa
     9361 ggacgagatc tcatcgtcgc caccggtgac ccgttcgata tagcgcggtt gcatgctctg
     9421 gaaatcgctt caggattgca agcgacgccg gtattggcaa aggacaagga aatctctgcc
     9481 cggatcgagg cgctcttcgg cagcaataca acctcagaca atacccaagg agccgtcggc
     9541 cgcgaaatcg agggcgccgt ggatgaagag gatgttgcgc atttgcgtga catggcatcg
     9601 gaagtgccgg taatccgttt ggtgaatcag atgctcgtgc gcgcgctaga gagtcgcgca
     9661 tcggacgttc atatcgaacc gtttgaaaac caactcaaag tccgttaccg aatagacggt
     9721 attttgcacg aagtggagcc gccgccgcgc cagctcaaag ctgcggtggt atcgcgcctc
     9781 aagatcctcg ctcagctcaa tattgcggaa cggcgcttgc cgcaggacgg ccggatcaag
     9841 atcaggttgg cgggaaaaga cgttgatttg cgtatcgcta cggtcccgac actttacggc
     9901 gagagcgtag tcatccggct tctcgagcgc gggcaaatct tcactcagct cgagaccatc
     9961 ggatttccct ccgatatact agatcacttt aatggaatga ttctcaagcc gcatggcatg
    10021 atactcgtga ctggaccgac gggaagcggc aaaacaacaa cgctttatgg cgcgctgcag
    10081 aagattaacg atcccggcaa aaagatcatc accatcgaag acccggtcga atatcaattg
    10141 agtggagtca atcagataca ggtcaagccg caaatcggat tgaccttcgc caatgggctt
    10201 cgctcgattg tgcgacaaga tccggacgtc atcatggtcg gtgagattcg tgatgccgaa
    10261 acggcggaga tcgcagtgca agcggccttg acgggccact tagtcttctc gacattgcac
    10321 accaacgatg cggcgggagc tatctctagg ctcctcgaga tgggtgttca agattattta
    10381 ttatcatcgt cgctgctcgg tgtgctggcg cagcgattgg tgcgtcggct ctgtaaaact
    10441 tgccgcaaac aggtttcatt ctcgaacttt gatagcagtg agcccgaact tagtctgccc
    10501 agcggaaatc tgccagagca ggtttgggaa gcagtcggtt gcaatatctg cagcggcacc
    10561 ggttatttag gccgggtcgg aatatttgaa ttgctgcccg tgacttctga gatctgcaaa
    10621 atcatcgtcc aacgcgccga tgccggaacg attcgcagtc ttgccgtcca acagggaatg
    10681 cgtctgctga gagatgacgg ctgggacaag gttcgtcacg gcgtcaccac agttgcggaa
    10741 gtccttagag taacaagaga agaggcataa cggattctct cgtttaggtt ttgtcggatt
    10801 cgcaagattt ctatctgaga gtcgatggcg ttctatcaat atcgggccgc agatcacgca
    10861 ggcaagatcg tcgagggcat aatggaggcc gaggccgagc agggagttgt gctgcgtctc
    10921 catgagatgg gttgcattcc gctgcgcatc ggtgcttctg ccgagcgtgc ggcggcggct
    10981 tcacaagccc gcttgccatt attttcgaag cgcaaaatca gccaacaaca attgctgcaa
    11041 ttcacgcagg agctgggaac attgcttgcc gccggtttgc cgcttgatcg cagcctgtca
    11101 attctcggca atctgattga aggagaagag ttcagtaaag tcatgcgcac gctccttgag
    11161 gcggtgcgcg ccggcaaatc cttggccgct tccatgggag agcattccga tgtctttccg
    11221 aaaatctaca taaacatgat tcgggcgggg gaatctggcg gaatccttga aggagtgctg
    11281 cggtatctag ctgagtacat ggagcgctcg ctggcgctca aggaagatct caaatcggca
    11341 ttgatttatc ctgtgattct cgccagcgcg gcgggagttt cgttgatagt gctattcctt
    11401 tacgtcattc cgcggtttgc agtgatcttt aaagatgtag gacaagcggt tccttggatc
    11461 acacgagtca tcatcggcct gagctctagc ttgaccgagt acggatggct aatcgcgatc
    11521 gctttgattg ccgctatcgc ggcggggata ttttatttgc gcaatcccga aacaaaatcg
    11581 gagctggatc gcgtttcctt gcgcctctgg ttaatcggcg atctggtccg caaatttgaa
    11641 acggccaggt tctcgcgcac cttgtcggcc ctactaaagg gtggtgtgcc tcttctcgaa
    11701 gctctgggca cggtgcaggg cattatggga aatagattga tggcacgggc gatcaaccag
    11761 gtgcagattc gagtccggga aggaaagggc atggcgcggc cattggcgga aagcggtttt
    11821 ttcccgccat tggcgcttaa tatgatcgct gtgggagagg agaccgggaa actggaagcg
    11881 atgctcgggg aggtggcggc gttctacgat caagaggtaa aacgaacgac gaaaagatta
    11941 acgtccatta tcgagccagt attgattctg gggatggggt tggtgatcgg tgtcgtggtg
    12001 atctccatgc tgctggcgat ctttagtatc aatgatttac cgttctaaaa aatatcttaa
    12061 caatgccaga gggttcacgc tcgtcgaact gatcgtcgtc gtgattatta ttggactgct
    12121 tgccggactc gtgctgccgc aattcattcg tcaagaagag aaagcaaagc tcaggaccac
    12181 aaaggcgcaa attgagcttt ttggcaccgc tctcgatacc ttccgtttgg atgtcggacg
    12241 ctatccgacc accgatgaag gtttgcaagc ccttcgcgaa agaccgggag gattggagcg
    12301 ttgggacggc ccttatttga gaaaggagct gccgttggat ccttggagta aaccctatgc
    12361 gtacagaagt cccggagagc acggcccctt tgacattgtg tcctacggcg ctgatggtac
    12421 acccggcggc gatggcgata atcgtgatat taccagctgg gaaaaatagc cgcggctttt
    12481 ctctcctcga gctgattttg gttctgatgg tcctcggcct gagcggactt attgttctgc
    12541 caaatgttga gaagggcttg cgagacaggg atttgcgcca gtcggcgctt agtctggccg
    12601 ccgcggctcg cgacttgcgc agccaggcgc ttcagaaggg cgtcccgcaa cgattggttc
    12661 taaacgtatc agagagcagt tatcggatcg cacgcaacga aatccattta ccttccaacg
    12721 taaagatcgc ctcggttgcc gggggcgagg ttctggacaa tggaagccgt caatttttgt
    12781 tttttcctaa tggcagcacg ttcggcggcc gaattgatct ctctaacggt gctgattcaa
    12841 catcttactc gatccgactc cacccgctca ctggtactat cgacgtgatg cggggcggta
    12901 actcgtgaga cataccacaa caggctgcgc ttcacgccga ggcttcactc tgctcgaggt
    12961 cgtggtcgcc atggctatcg tcggactcgg tgtggtaacc ctgctggaaa tattttcatc
    13021 aggtttgcaa ctcggcgctc gaagtcgaga tcgtacggaa gcggttgcgt atggtcgtca
    13081 ggtgatggat cagttcttgg ctcggcgcac ggttgctgaa ggcaccgaac agggccaaat
    13141 cggcgagtct agccggtggc agcttcaagt ccagcctgtc cgatcggttg aagggctgac
    13201 gctgggcaaa gattgggagc tgaaagatat agcgctggag attttcgttc cggaatcagg
    13261 tcgcgaaagg cgggtcgaac tgagaacgct ccgtttggtg aagaaggaag atcgttgatg
    13321 agagcaccgc gtctcggccg gaactcatgc ggcttcacct tgattgaagt cgttctagct
    13381 ttaagcattt ttgctcttat aggagccgtc ttgtacggcg ccttttcatt aagtcatagt
    13441 gctgtcgaaa aatcgcaggc tagctttgcg gataatcaaa agctgcgatc gttcacggat
    13501 ttgctgggaa gctatgtccg atcggcgtac ccatatcgag aatctgcgca gcaggcatcg
    13561 gtgttttttg atggacagga agaccaactc acgtttgtct cttcgatctc attggccatg
    13621 ggaggtcgag ggatgtcaaa gattcgcgtt agctgggacg gcgccgaaca aggcgggggt
    13681 ccgattcagc tcgaagaaga actgccggtg cgtttgaacg tgaatgaatc tcaggatgac
    13741 ggcgggcttc gaaacgaagt ggttctacaa gagcgggtga aagagcttcg gtttgcctat
    13801 ctggacccca agagcgaaga cgaacgatgg gaggaacgct gggatggaag agaaaagatg
    13861 gcgcttccgc gcgccgttcg cctcacctat cgcgatcgtg atggcagggc aatccgctgg
    13921 atctttccgg tgatgatcag cgttctggcg cagtgaagat cgtggaattt ctccaaagaa
    13981 aattggccgg cgtctcgagc gagcgtgggg ttgcgctgct tattgtcttg tggattttta
    14041 tttttctttt tgtagtggct tttgatttca gcgccggtgt gcgcgaggaa gccaccgcag
    14101 cgcatcgcta cagcgacgac aatcaaggat actatttggc gctagcaggt ttcgaacacg
    14161 gcttatacga atttctcgat caagcacgag gccgtgagtt acagccggtc gacaagccaa
    14221 aagacatttt cgacgccggc tggcgtgagg cgaccctcgg gtccggaaca taccgtgtcc
    14281 ggctggtgga cgagagcagc aagatcaatc tcaaccgagc ggatgagaat ctgctgcgcc
    14341 ggattttttc aaatctcggg atcgaagaat ctctcattgg cgtcttgata gattcgatca
    14401 tggactggcg tgatcaggat gatcttcacc ggtctaacgg cgcggaagac gactattatc
    14461 tatcgctcac gccttcctac accgccaaga acggcccgtt cgacagcctg gaagagttac
    14521 tgtgggttaa aggcgtgagc gccagcctgt tttatggtta tgagagcagc ggtagtcggg
    14581 atcgtgactc gccgcgtgtc ggtttgcgag agatctttag cgtcgacagt ccgattgacc
    14641 gcgtcaatct ccgcaccgcc tcggcgccgg ttattcacgt tttgctcggc atatccttgg
    14701 agaagtgtca ggcattcgtt gaggaacgag caaagctcgg cgataaaact ctcgcagatt
    14761 tgctgccgct tttgggaatt ggctccagcg aggcggcgct gcagatgttt atcttcacta
    14821 gtccgagcat cgtgacggtt gaggctgaag gttatgcgag tgaatcgcgc cagccacgac
    14881 gcgtgaaagg agttatccgc gccggcggcg gcaaccgcgg atatgagttg acccgctggg
    14941 tggaccgaga aattggctac cccactgacg gacaatgagg tacaataggc agcttttgtc
    15001 tcaaaatggc aaagcccagc gcaatcttgc aaagttaaat tatgaacttg tctaactcga
    15061 tcagaaatct tccacgcgcc gattttctca gaagtgtcgg gatttacatc atgcgcgacc
    15121 atttagtgtt ggttcggctg cgcaaaaact ttcttaatgt gacgctgctc gaacaagagc
    15181 tgagagattt acctatcggt gaaaaccgcc aagcgatctc ggagttgacc ggatggattg
    15241 ctgaagatgt gcgtgagatt gcgctgaaag ccgaatatga ctcgcgcgag cgggcgttgc
    15301 gccaggcaat tctatcgctc ttgcctcatt tcagtgcggg cagggattcg ctctatatct
    15361 gtgttccaca agatcaggcg atcattcaaa cagtctatct gccgcaggcg gccgaggcta
    15421 atcttcaaca ggtgctcgaa tacgaaattg aacggcagat cccgtttaac cgcgacgaaa
    15481 tctattacga ttacctgccg atcggaaagc gtggcgataa gatcggcgtc cacctcttcg
    15541 ccgttcccaa gaaacaccta gccggcgtac tggacgtcct gggatcgttc ggtatcgttc
    15601 cgagcggagt tgaaaccacg gggacggctc tcgcgaacta tttgcttttt tgcaaaaaag
    15661 acttcactgg ttgcgcggcg atcgtcggcg gtcacgccga tgagcgcgaa ttgatcggag
    15721 ttcgagccgg cgctgccggt tggacgcaag cgccagaact gcttttttca cacagattac
    15781 ccgaagctga gtgggccaaa ggcatcggta aggaactcat taccgagtgt ctgcgtcaaa
    15841 cgccagaact atacggctgg ggaaacccgg atcaggtcgt tggttctgtg gatggcgaaa
    15901 aggctcagtg tgaagatctc tgcgctctgg gaatcgaaaa actcggtggc agctcgcagt
    15961 taaccgctac cacgctgccg gctgtcggcg ctgcgctccg tggtgttcgg gaagcccgct
    16021 tggagaccaa ctttctccgg ccacctggag cggagcgcgg cgcgcgaaaa aatctctcac
    16081 tactgaactc agtgatgatc ggcttgttga ttctcttgct tgtgggctgg ggaatcagct
    16141 acccgatcaa ggacgaattg cgcctaaaac aactgcagca ggagaaccat aaaaacgagc
    16201 cttatgtgga agccttacgc cgcgaggagg ttcagctaca gacggtgcgc aaggaagtac
    16261 agtttctgtc ggatatcgat cgacgtaagg gcgaggtcct gcgcgtgctg gatgaacttt
    16321 cgaaagtggt gccgaccagc gcttatctgt ctaattttcg ttatcgcaac ggtgtcttgg
    16381 aagttcaagg caatgccgag aatgcgtcta cgttgattcc gctgttggaa aaatcgccgc
    16441 tctttgaaaa tgttgcattc aatgcgccgt caaatcgcgg ccgtgataat cgggaaacat
    16501 tttcgttgaa agcggacttg gagaaacaag ccaccagcgg tccgaagtta cccccctccg
    16561 agaagtcgaa agagaaggcg ggtaaactat gaaacagttt tggggaagat tatcgagacg
    16621 ggaacgccac ttggttataa ccaccgccgt gattctatta ctcgtgctcg ggcgtctttc
    16681 ggtggtcggg ccttatatgg ctcatcgtga gtgggtgcag actcagctgg aaatccagcc
    16741 gcagcttcta gaaaagaact tgcgctacat tggacagaag gaacagctca cggcggcctt
    16801 agaagcggcg cgaaatgacg taaaagttca tgagaccaag ctattgacgg gcgatacgcc
    16861 atctgtgaat gcctcggact tgcaagagac ggtgcaagct ctggcggcta aagaaggtac
    16921 tcaggtgatc accacacggg tgctgaatcc ggaagcgacc ggagcttttt ccaaaattgc
    16981 gattcaaatg gaagttggcg gtcaggtcga tcaggtagct tctcttgtgc gtggcattga
    17041 gacttcaccc aagctgttag tcatcgatga aatcaatatc cgttcattgt ttcgtcccgt
    17101 cggattgccg caacaacaag gcgcgcccca agcggcagca caaaatctgc gcgttagtat
    17161 tactgtcgca gggtttgccc ggggacaagc tactggggca aaatcggatg cgccgaagac
    17221 aactctagaa aagggcgtgt gagagcggag cacttatctg agcttccatg ctaatgcgat
    17281 tggccgggat ggctcctgag aaaagaagtt caagtttgtt gacgctgaag ctgaatgacg
    17341 tcagcttggg ctctttgttt tgctaacaat tatccggagg atttcgagat tctaaaaagg
    17401 tcgtcattgc tgagcattct tcttttggtc gccacaggtt tggtcggctg gcgcatctac
    17461 atggtttggc gggaggagcc gtggagtttg ccgcagccta agacgatggc tccagcagcg
    17521 ctacaagatg agccggccac cgtcgtgccg ccgctgccga ccgtaactcc cgatctgatc
    17581 attagcaaga atctcttcga tccggagcga ggcgcaggtg caactcgaga agcagagttt
    17641 aattctcgct cttttcagcg aatccggaat atggtcctac ttggcaccgt gatcctcggc
    17701 gacagtcgtt cggcgattat acaagatccc ggcagccaag tgtctacgcc caacggatca
    17761 gccgcatccg ctgggccgat gcgcgtgacg ctcggcgaca gtgtcgaagg atttcgttta
    17821 accgaaattg gcgacaggag agtggtgttt acgaagggta gctcgagggt cgaagttgtc
    17881 ttggactatt tccgaagaga tgatccggca cccgctcgag cagctacagc gccggttcag
    17941 cgcggcgcgc cggcggtcgc gccgagagtc gttcccaacc tgcccagaag gggagcgctt
    18001 cccgctccgc caaatccaaa tcccaaatct taatcagcag gaggattgat gccaggaaag
    18061 tgttttagag gattggtgac gttggtctac gtcactggca gtgtgctccc gctcgctgca
    18121 cagcagggta ccgcggagcc tcccctgaca cccgtcccgg tgcaacccgc tccgacagaa
    18181 ccgaatcaga cgcagccggg ccaggggcag ccatcgccaa gccaacttcc gcgacgcgcg
    18241 cccatagttc cccagcgtcc cccgtcttca gcgcaacagg tgccaggaac tcctggccca
    18301 tcggacggaa gtgttcctca gggtatcccc acgcccgcac cgacgccggg tccgacgccg
    18361 attccgccgg aacgaaggat gttaccgccg ccaagcacgg cgggcggcat ggtctcgctg
    18421 aatttcaacc gagccgattt agtcgagatc attcacatat tggcgcagca ccttcggctg
    18481 acatacacca tcgatccgga ggttaagggg attgtcacga ttcacagcgc cgagcctctg
    18541 cgcccggaag atctgttgcc cattttccat caagtcctgc gaatgaacaa tgcggtggcg
    18601 gtgcgcacgg gaaacatcta tcggatcacg accattaagg atgctaaagg gttagtacgg
    18661 ccggtggcgc aaggccgtga agacagcttc gcgttgcaaa tcgttcccgt gcgattcttc
    18721 tcggccgcgg agatgaagaa gcttttgacg ccctttttga cgcccggcgg ggagctcgta
    18781 gactatccgc gaggaaattt tctggtcatc atcgatcttc cttctaacat tcagcgctta
    18841 gtcgaaatcg ccgatatgat cgacgttcag gtgtttgcgg gaacgcgcat ggagatctat
    18901 cagccgaagg ttgccagcgc cgaggaactt gccgccgaga tgaccaaagt gatgcagtat
    18961 tacgccgcct cggcgccgca acaagagaac ttcgcttctc aattcgttcc cttgccgcgc
    19021 atcaatcagc tgttggtgat cagccacagt gaggcggcgt ggacttacgc gaggaggtgg
    19081 ctggagcgcg tcgacgttgc tggcgaagga cccggccggc gaatcttcat atacccggtg
    19141 gaaaacggca aggcgatcga gctggccgat gttttaaccc aggcgctcgg ccagcccacc
    19201 acgggcagaa gagaaagttc caggacactc caagacttgc atcgatccac ttccggcgga
    19261 ttcggacaat tcgatagcgg cacccgttcg tcgagttcac catttggacg ttcaggtttt
    19321 cagcaacaaa accagccatt aggcggcgcc tatgccaccg ttccggtacc gggccaagct
    19381 ccaccggcgg cgccagcgcc cggagtcgcg ccaggtctac cgtcggtgcc gggagctcca
    19441 cgaccgccgg cgccggccgc tccaggcgct ccaggagcca agccggaaga acaattgagg
    19501 atcgtccctg atccggggac gaattcccta attatctacg gcacggtgca ggagtttcaa
    19561 aatataaaaa acatattgaa agaactggac atcatcccgc gccaggttct catggatgtt
    19621 atggtggcag aagtgtcgct aacgggttcc gaagcattcg gtgttgagta tgaattgttt
    19681 cgccaacata acgcgtcgat cttcggtcaa actttcgact ctcaaggagc agtgagaagc
    19741 gggattttca atcaagcaac tcgccccgcc accgagaatt cgccgtttgc tcagttcttg
    19801 aacggcatca gtggcgttgc cggtcggggc aacaccatcc gcgcctttat ctcggcacta
    19861 gcgaccgaca accgcgcaaa gattctctcc tcccccagtg tacttgcgac cgataatcgg
    19921 ccggcccgaa tccaagtcgg ccgtgagatt cctgtattat cgtcgcaatc acaatcagtt
    19981 gtcggtgaca gcccgatcgt aaacagcgtg cagtaccgga ataccggcgt gattctcacg
    20041 atcattccgc aggtcaattc acagggatta gttaacctcc aggtcaaaca agaagtgagc
    20101 gatgttggag ctccctcttt tggcaatacc aactcgccgt cgttcacaac ccgagatgca
    20161 gagaccaccg cagtggttca agacggcgat accctggcga tcggcggcat tatcagcgac
    20221 accacgaggc gagatcggag cggagttcct tacttgatgg atcttccagt cttgggccgt
    20281 ctttttggta ccactaacga cacgacggac cgtactgagc tggtgatgct tatcacgccc
    20341 catgtcgttc gtaaccggga cgaagcccag cagattactg aaggcttcaa agagaagctc
    20401 tatggtgtga gaaatgagct ggagcgtttt gagttggaac aggagaggtt gaagtcacga
    20461 aggcagcaat ctcccatgcc gccagccgac attccggcag tgccaggagc agggaataaa
    20521 ataccgaatc cagctccgaa cacgtcgtct attccaaggg atttaactcc atcgatcccc
    20581 ttacaaggtc tgccgcagca cacgccgcag aatcctgcgc cgccccagcc ctccatatcc
    20641 ctaaataaac gcggcaggaa aaatgcaccc ccggtcgata cggccagtac ggcggtcaca
    20701 ttcaatcggc ccgcagaggt cgcggtagta accgagttga ctaaccgcaa ccctccgcta
    20761 ccgtggcccc cattagtgcc ctcgtcgccg actgagtggg ttgcgcccgc tgatccaatg
    20821 caagtcgcac aggtcccgca agctgaaagc ataggctcgg cgttgtcggt tcgctctatc
    20881 gatccggtcg cgcaattcga accggcgccg acgaaagcga gcataggcaa tccggtgcct
    20941 gccatggacc gggtcatcac aatcgcaaaa aagatcgatc gcgcaactcc ggcggccgag
    21001 atgagtcaca tctggatggt tcaggtcgcg tctctgccgc aggagaaaga tgctgatcag
    21061 atggcggcaa cgctgcgtca gaaaggctac gatgcgcgcg tcattaaggc cgaggttgac
    21121 aatcgattgc gctaccgcgt tcgtgtcggt cagttggcga cccgaaatga agcggtcgac
    21181 ctaaacaatg cgctcaaggc aaacgagaag ttcgccgata gctacatttc gcgggtaact
    21241 gttagataac caaatctctt ttagactctt caggatcctc cttcctattc tccgagaatt
    21301 tcgtctcgta ccagcacggc ggcggttgcg ccgctgtgct ggttagtctt acgccggttc
    21361 ttattcgact gggaccaatc tccaaagctt tccataacct ggagcgcttt gttatgatat
    21421 ttatagagat tgtacgacgg ttcggtagac gcatgacagt gtcgatttct gagttgaggg
    21481 tactcattcg cggtggcggc gagatggcta cgggcatcgc gcaccgcttg caccgcagcc
    21541 atatgcgggt gttgatcact gaaatcgcct cgcccacctc ggtgcggcgc aatgtcgcgt
    21601 ttgccgaagc ggtttatgaa ggcgctcaga cggtcgaagg ggttaaggct gtccgggttg
    21661 attcctatga tcaagcctac gtcgcttggg agcaaaacca gatacccatt tttatagatc
    21721 ctgaagcatc cattggaaaa atattaaaac cggtggtcat cgtcgacgcc atcatggcta
    21781 agaagagagg agttgcaaaa cttactgatg cgccgcttgt gatcggggtg gggccgggat
    21841 tcacggcggg tctcaatgtg catgcggtcg tcgaaagtaa tcggggtcat cacttaggcc
    21901 gggtcatctg ggatggggct gccgagtccg acacaggcat acccgcttct gtggatggct
    21961 acacagcatc tagagttctg cgggtgccac ggagcgggat cttcagcgcg ttacgagcga
    22021 tcggggattc gatcacggag ggcgagccgg tcgcgacggt gaacggtgca ctgattaaag
    22081 cagagatctc gggtcagatt cgtggactgc ttcgaaacgg cattcacgtc gagaaaggca
    22141 tcaaggctgg tgacattgat ccgcgtggtg aaaggggtta ttgtgattcg atttccgata
    22201 aatcgcgagc catcgccggc ggcgtgttgg aagcggttct tcattctttc agggacttaa
    22261 attcatgacg atttcaaatt ccaaaatctc aaacggtgag gctggcgtga atccaaaaca
    22321 gctaacgatc gacgacgttc aggagattct cctcaaagag gaatatgttt gcgaccgctc
    22381 actggctacc gtggtgtatc tttcactcgc aatgggtaaa ccgttgctcc tcgaaggaga
    22441 ggccggagtc ggtaaaaccg aaatagccaa ggtgcttgcc aaagtgttga acgccaaact
    22501 gatccggttg cagtgctatg aagggctgga tgccaatacg gcgctgtacg agtggaatta
    22561 tccaaagcag atgttacgga tcaaactgga agaggttcaa agaggggata aaaaggcggt
    22621 cgaaaccgag atcttcaacc aagattactt gctcaagcgt cctttactgg aggctattat
    22681 cagtgaagag gagaatccgc cggtattgct gatcgacgag atcgatcgcg ccgacgtgga
    22741 gtttgaagcc tttttgctcg aagtgctttc cgacttccaa atcactatcc ccgagatcgg
    22801 caccgtgcaa gcgaagcagc gtccgtacgt ttttttgaca tcgaatcgga cccgcgaaat
    22861 tcatgacgct ctaaaacgac gctgtcttta tcattggatc gagtatccaa cttttgaaaa
    22921 ggagctggag atcgtttcga ccaagtttcc agaagtcgcc cctgcgatgg ccaagcagat
    22981 ctgcgctttt atgcagcgtg ttcgggaaat gaatttttac aaacggccgg gggtcgccga
    23041 aaccctggat tgggcgacag ctctcattgc gcttaaccgg aaggggttgg acgacaagac
    23101 tgtcgttgag acgatgggct gtgtattcaa atatcgagaa gatctacatc atttgaaaga
    23161 gcagatcgaa agcaagcatc tcaatttgga tgcattgctc aggtcttcgc ccgagttgat
    23221 gagttagcgg atatggaaat cacaccggaa agcgtagcag ctgcggtcaa caattttgcg
    23281 ggggtgcgcg gacatggtac cgtcgcgaat ttactcgctt tcggacgtgc tctgaagcaa
    23341 ttgggcgtca agataagctt gagccaagtc attgacgcgt cccgatccct cgatttggta
    23401 gacatcgccg agaaggagga ttttcgtgct ctcctgcgcg ccaatttgat atcccaagtg
    23461 gaggattttc ctatcttcga taccgtcttc gattactttt ggcgtgaaca gaattacgag
    23521 cgggtgccga tggaaacgct tgagattgag ggaacgccta ccgaatctca ggcgcaagaa
    23581 ggtggtgagg aggagggttt ggatgaagcc tttgctgaaa ctcccgccaa agagaacgtc
    23641 gagctggaga acctggacga gttttcaatc ccgacctaca gcgctcaaga gttgctcaac
    23701 cgcaaagatt tcagtgaaat gagtgtcgaa gaaagtcgcg cgatcgcgcg agccattctg
    23761 ctgattgcca cgagaatcgc gacgcaagtc agccggcgca agaaacgcgc gcgcaaaggg
    23821 gcaacggtgg acccgcgctg gaccttgcga aaaaacatca agtacggcgg cgacgtcgtc
    23881 gagttggtca accgcaagcg gcgcatcaag aaaaccaaag tcgtgctgct ttgtgacgtc
    23941 agcggctcga tggattgcta cagtcgattc ttgattcagt tcatgtacgg attgcagaat
    24001 gagttgtggg gtgttgaaac atttgttttt agcacctcgc ttacccgtat aacccatttg
    24061 attcgcacca aggatattgc caacgcactg gaacagatct ccggcagcat tctgggctgg
    24121 tccggcggca ccaatatcgg tcgttctctg cagaccttca accgaaattt tgctccggcc
    24181 atggttacct ctcgaaccat cgtcgtcatc atcagcgatg gttgggaccg gggcgacgtc
    24241 agccttttgg agcggcagat gcaggaccta aagcgtcgat gcaagaaagt gatctggctc
    24301 aatccgctgc tcgccagtga aaattatgag cctttgtgca agggaatgca ggcggcacta
    24361 ccctacatcg atttgttttt gtcggttcac aacctgaaca gtctggtcgc gttgggacgc
    24421 actttacaga agatggtggc ttaagtttca cggaggaaaa ttatgtcagg ccaaggaaca
    24481 tgttttgaga accccgagtt ggaagccaag aagacagact cggtctatca ccaggtaaaa
    24541 gagtttctcg acaaaggtga aacagtggct gttgcgacca tcgtgtcggc caaaggctcc
    24601 acgccgcgag aagtgggcgc taaaatggtg gtcacggcct ggggagaaat tctcggcacc
    24661 atcggcggtg gttgtggcga ggccgatgtc aagcgggaag cgattgaagt tatccgcact
    24721 cgcaagccta gattggtgcg cgtggatctg cttgatgaca tatcgtcaga cagtccggct
    24781 gtttgcggcg gcattatgaa tattttcgtc gatccctggt gggaagggcg cgatgaggag
    24841 ccagcggcca gcgaatgagc gccctttctg aagaattggt gcggatcagc gagcaaggtt
    24901 tgtccgcggt tctggcgaca attgtcgagg cccagggaag cgaccgtgtc caagccggcg
    24961 ctaaatgcct cgtagtcgat ggcgagatca aaacggacac cattggcgat ccccgcgccg
    25021 tcgagaagat tgttcgtgag agcgaggcga ttttaagctc ggagaagtct aaactcgtcg
    25081 cgcttgaaac tcccgatggc ggaagacttc aggtcttttt tgaagtcatg ctaacgccgc
    25141 ccaagctgat cgtcgtcggt gccggccata ttgcagtgcc tctggtgcaa atcgccaagg
    25201 ttctcgattt tcatgtcact gtcatcgacg atcgtctcct ttacgccaac cgcgaaaggt
    25261 ttcctgcggc cgatgaagtg ttagtcggcg atatggcgca aatgttgacg gagatgacga
    25321 ttacgccttc cttttatatt gtcctgatca cccgcgggca taaatacgat gaaccttgtc
    25381 tccgtcagat ccttcacagt aaagcgaaat acatcggcat gatcggtagc cggcgccgaa
    25441 tcaaggcctg cttccaacga ttccgtgacg aggagaaaat cgccgaggaa gtgatcgagc
    25501 gggtctacgc tccgatcggt cttcatatcg cgaccgagac gccagccgaa atcgcgttgt
    25561 cgattctagc cgaggtgatt aaagttcgtc gcggcggcaa ggcggcatcg ctttccggcc
    25621 attgagccgg cacttgaatg gacggtgcca gtgccgcgca aaattcaggg cgtgattttt
    25681 tcggacctgg gccaagcgtc caaatttatg gcgctcgact gggtgcagga cgcgcttcgg
    25741 aaagctttag gctttgcgcc atacccggct actctcaatc tgcggccatc aacccctcag
    25801 gatgcgcggc agtggcaggc aatccagaga gaagttgcgc cggagccaat ggcgccgatc
    25861 gagagcggct tctgtaatgc cgggctttat tctgtcagca tacagcggtt gattcgaggc
    25921 gatgaaacgg tcaaaggagc tgtactgctt cctgctgtga tcgattaccc cgaggataaa
    25981 atcgaaatcg ttgccccggt tcgattgaaa caagcgctct ctgtcgagga tggtgactcc
    26041 ttggttttgg agtttaacga ttgaataaca aaaaacgagt gatcgtcggg atttcgggag
    26101 cgaccggcgc catctatggc gttcgcattc tcgagatcct ggccaaattg gctgacgtcg
    26161 aaactcattt ggtgttgagc aaagcgggtc ggatgacgat ccaggttgag acgccgtact
    26221 cttctaaaga tgttgaggcc atggccgatg tcgtccacga cgtcaacaat gtcggagcga
    26281 gcatctcgag cggctccttt cggaccgaag gcatggtcat cgcgccgtgc tcgatgaaat
    26341 cgatgggcgg tatcgcgcat tcgttgggcg gggacctcct ggtgcgtgcc gccgacgtgg
    26401 ttttgaagga gcgcaaaaag cttgtgctgg tggtacgaga aactccgcta cacttgggcc
    26461 atctcgaagc gatggtctcc ctaactcgaa tgggagcgat tatctttccg ccggtgccgg
    26521 ctttttatca ccgtcctaaa acattggacg acgtcatcaa ccaaacagtc acccggatcc
    26581 tggatcagtt cggcatcgac gtgaaattgt ttcatcgctg ggatgacgag ggaatgagcc
    26641 gacatcctga ggccggaaaa accaccacgc tggcggctgc taaaactagc cagcgagtta
    26701 aaaagcagtc gtagatcgct gctttgctcg acatgccaag ccgagagaac aagactctca
    26761 ccaagacgca acagcggaaa aaagaacatc tcgagctgtg tctcgacgcc aagaccgttt
    26821 ccgcgccaac gggatccggt cttgattcgt atatgtttct ccacaatgct ttgccggatc
    26881 tcgacctcga cgaaatcgac accgagacaa cttttttggg caagcgactg aaggcgcctc
    26941 tagtcatctc ctcgatgacc ggcggtttca gcctggcccg aacggttaac cgcaaccttg
    27001 cagccgcggc gcaggagctc ggcttgggga tgggggtcgg gtcacagcga gtggcgatcg
    27061 aggagccgtc cgcggccgat tcgtttcaag tcagagacgt cgcaccggat atcctcttgc
    27121 ttgccaatct cggtgctgtt caactcaact atggttacgg tgtcgatcag tgtcgacggg
    27181 cggtggaaat gattggcgcg gatgccctga ttctccatct taacgttctc caggaagcag
    27241 tgcagccaga aggcaatcgc aatttttccg ggttaacggc taaaatagcg gcggtctgtc
    27301 gtgatctgga agtgccggtg atcgccaagg aagtcggcag cggcatctcg gccgaagttg
    27361 ccctgcggct tgctcgtgcc ggcgtcaaag cgatcgatgt cgcgggaaag ggaggcactt
    27421 cctggtacgc ggtcgaggct tccagggcgg ccaaggccgg gcaaccggtt gatgtctcct
    27481 ttgccgattg gggaattcca accgatcaat cgttattagc catacgcgaa gttctcccgg
    27541 aaatgacact ggtcgcttcc ggcggcattc gctctggctt ggacatggcc aaagccatag
    27601 ccctcggcgc cgacctagcg gctttgggtc aaccgctgct ggcgccggcc ttggagtccg
    27661 ccgagaaagt ggtagcgtcg ctgcgttcga tcatttacga gatcaaggtg gcgatgttgt
    27721 gtgtcggggc tcacgacctt ggcgccttgc gtagaactcc actgctgcag cggccgctgt
    27781 gagctactca acgatcggtt cgaatttaca gaagcctatg cttctttagg ccatggcgtt
    27841 tcatgcctga tgatttaaag cagcagctca tccagtatct ccagactcat aacaccatga
    27901 ctttggccac ttgcgccgac gagcggccgt gggcagcgac ggtcttttac gccagtgaag
    27961 aatggaagct ctattttttc tcggcgccgc aatcacgcca ttgtcaaaat cttgccgcca
    28021 acgctggggt tgcagtcacg atccaagagg attacaagga ttggcgccgg attaaaggaa
    28081 ttcagctgga aggaaaaggc ctgccggtgg attcaatcct cgagaaaacc aaagcgatgg
    28141 ccgtctatgc gcgcaagtat ccggagatta tcaagctttt cacgaccccc ggcagtggcg
    28201 tcttttatca ggcgtttctc aaagtcaaat tttactgcgt cgtccctgag aggatatact
    28261 ttatcgataa cgagcagggc ttcggtaaac ggcaagagtt ggaattgacc gggcaggtgt
    28321 agcaaggacg aacaatgtcg caagtactgg ggaatgagtt gacggtagag ctgttgagcc
    28381 gattggatgg acgggatgtc gaatcccatg agggtaagat cattccgatt gttacggtgg
    28441 atgaatacgg ctggccgcat ccggctttgt tgtcgtatta cgaaatggtc gcgaaaaatt
    28501 cacgtaccat cgatatggcg ctgtggaaaa acagctctac ggcgaataat ctcaggcaga
    28561 cgggtaaggt gaccttgatg atcatcgacc acgcgttgaa ttattatctg aaggggaccg
    28621 tgagccagac cgaagctgaa atgtcgggcg ccgctgcggt ttcgcgcttt cgcgtgactc
    28681 tggagcaggt gatcgaggat caggaaccca atgctgagat aactaccgga ttgacttatc
    28741 gccgaatgac tcggcgtgac cccaacgatt ttgctgcgaa agttttccga ggcttgaaag
    28801 cttgaaagaa agatagtgaa tcggaagaag gccattgctt ggttggcagc ggcaagcgtt
    28861 atcggtcttg gtggcgtcgg ctggtggcaa cttggcgacg gttctaaaga acctccgtac
    28921 gttaccacac cggcacaaag ggcgaatatc acgcaagtgg tctcctcgac cggcactctg
    28981 caggccgtcg tgactgttca agtcggcagc caggtttccg gaaccatcga aaagctcttc
    29041 gccgatttca acaccaaagt aaaagccggg cagattgtcg ctcagctcaa tcaggataaa
    29101 ttcagtgctg ccgaagatca ggctcgcgca aatttgctgg cggcgcagtc gaacatcgag
    29161 aaaagcaagg ttagcgtagc cgatgccttg cgcacgctcg agcgtaatcg gcagctgaga
    29221 acgaaagaat tgatggcgca aagcgatctg gacgcggcgc aggcggccta cgacgccgcc
    29281 gttgcccaag tcgaagtgaa tcgggcgcag gcggcgcaag cccaagcctc attgaagcaa
    29341 gcgacggtag atctcaataa cacagtgatt cgctcgccgg tcgacggact ggtgatctca
    29401 agaaacgtcg atgtcggtca gaccgtggcg gcttctcttc aggcgccgac gctgtttaca
    29461 attgccaacg agttggcgcg aatggaagtt cataccaacg ttgatgaggc ggatgtcggt
    29521 aatgtcagag aagagcagga agtcacgttc acggtggatg cctttccgtc gcggcgcttt
    29581 cgcgggcgcg tccatcaagt gcgcaatgcg cccattgtcg tgcaaaatgt tgtgacctat
    29641 gtagccgtcg tccgcatcga caacaaagag ttgctgctca aaccgggtat gactgccaac
    29701 gtgcaatttt tggtcaaccg aaaagaagac gtcctcacta tccccaatat ggcgctgcgc
    29761 ttcaaaccgc cggaagagaa agaggaggcg caggaacttc tacgccgcga acagagccga
    29821 accgcggcga aagtaggcga gaggcggact agccggcaga gcgtggcggg cgcgggtgcc
    29881 gcagccgttc gcggaacgag gcgggtgcgc atctatgttc taagaggaga taaagctcaa
    29941 ccgatcgaaa ttcagctggg catcaccgac ggatcaaaaa ccgaggttgc cagcgctgat
    30001 ctgaaggaga atgatccggt cattatcggt atgagtagtg gcgcaacctc gggtaatccg
    30061 ggggtggcca atccgtttca gccgccgccg actcgcggct tcggttttcg atgagtgagc
    30121 tgatccgcgt caaggaactg tgcaaaacct atcgcatggg tgacgtggag gtgcctgcgt
    30181 tgcgtggcgt tactctcgcc atccgacaag gtgaattcgt cgccgtgatg ggatcttccg
    30241 gctccggcaa atcgaccttt atgaatatac tcggttgtct cgatcgcccg accagcggga
    30301 aatattttct cgatggcgat gaagtcgggg cattgacacc ggatcagtgg gcggacattc
    30361 gtaaccgccg tatcggcttt gtgtttcagg gattcaatct gcttgcacgc accactgccc
    30421 tcgaaaacgt cgaattaccg atgatgtaca acggctgcag cggcaaacaa cggcgtcagc
    30481 gggcccagga agtgctcggc ttggtgggcc tcgatgagcg tctcgatcat atgccgaatc
    30541 aactttccgg cgggcagcag cagcgggtgg ccatcgccag agctctggtg aaccggccgt
    30601 cattgattct tgcggacgag cccactggaa atcttgattc cgccaccagc ggcgagatca
    30661 tgggattctt tcgaacactt aatcgtgatc aggggattac gattgtttta gtaactcacg
    30721 aaaccgatat tgccgaatac gctcagcggc aaattcattt cagggacggt tcggtggtct
    30781 cggatcatgg aaccgaatca cctcacttat caatcgagca ctgagccatg ttgttcatga
    30841 cactgcggat cgctctgcgc gcgctgggaa gaaacaagct gcgtgctttc ctgacgatgc
    30901 tcggaattat tattggagtc ggtgcggtca tcgcaatggt tgcgatcggt gaaggggcca
    30961 gagtcaccat tcggtctcag atcgccagtt tgggaactaa cgtgctgatc gttttacccg
    31021 gaacgacggc gcagggtggc gttcggacgg ggtcgggagg cgtcaatacg ctggttgatg
    31081 gcgatgccaa agcgatgatg cgagagctgc ccgccgtcgc ttccgcttcg cctgctttgc
    31141 gtcgtcctga gcaggtggtg gcggggaatc tcaactggtc gaccttaacc caaggggtgg
    31201 cgccggaatt tcagcagatc cgcgattggc aagttgccga gggccgtttc atacacgatg
    31261 gcgatgtcga aagcgccgcc aaggttgccg ttatcggcga aaccgtcctg aatcagttat
    31321 tcggtaatga cgatcccatc gatgcagtca ttcgcatcag aaatatcccg tttcgggtcg
    31381 tcggcgtgct tgcgcccaaa ggacaaacga gccagggaac cgatcaagat gatacaatta
    31441 ttattccgta tacgacgatg cagaaacgct tgatgcgtat tactttcgtg caaagtatcg
    31501 tcgcctcggc cgtcaacgcc gaacgccttc cggaagccca agcgcagatc actgcgcttt
    31561 tgcgccagcg acatcgaatc ggcccggacc gtcaagacga tttttacatc cgtaatctct
    31621 cggatatcgc cgaggctgcg tcgagtagcg cgcgagtgat ggccgtgctg ctgggctcgg
    31681 ttgcatcgat atctctgctc gtcggcggta ttggaatcat gaatatcatg ctggtgtcgg
    31741 ttaccgaacg tactcgtgaa attggcattc gtatggccgt gggagcccgc cgcaaagaca
    31801 tcatgctgca atttctggtc gaggccgtgg tgatggccgc aaccggcggc tctatcggca
    31861 tattgttggg gatcggcagc tccgagatcc tcaatgactg ggtacaatgg ccgactcaca
    31921 tcagcgcatc gattatcgcc ctcgcttttt tcttttcagc cgctgtcgga atatttttcg
    31981 gcttctatcc cgccaggaaa gcagccactt tagatcccat cgacgcgttg cgctacgagt
    32041 gaagtgaaac tagttcgaaa tcgatctcgc tgcgaaacgc attgatacgc gcgaccttga
    32101 ccctcacgtt atcgcccagc cggaacttcc gcccatgacg gaggcctttg atcaggtatt
    32161 ctttctcgta atagtagtaa tcgtcatcgg tcagcgaccc gattttcacc agcccttcaa
    32221 cgaaatagct gtcgagctcg acgaagaatc cgaagttcgt caagctcgtg ataaagccgt
    32281 tgtactcctc gccgatctta tccatcatga attgcgtttt cttcagggcg accatttctc
    32341 gttcggcgtc catggcattg cgctccttgt cggaggtgtg tttgcccgct tcgacgagat
    32401 aggcggacaa attttctcgt tcggcacttg tcactttctc atcgcgaagc gctttctcga
    32461 gcatccggtg cacgatcaga tcggggtagc gacgaatcgg cgaagtgaaa tgagtgtagc
    32521 atctggaggc cagtccgaag tgtccgatgt tttcgggttg atagagcgct tgtttcattg
    32581 ctcgtaagag aacacgattg agcactctct cctcgggctt accgcgagcc gcctcgagca
    32641 gcttttgtat ctccagcggc gcgatcttgt ctttcttcag cggcagccga tagcctaggc
    32701 tcagcaggaa cggcgccacg gcttcaagag attccttctc tggtccctcg tgcacgcgat
    32761 aaagcgctgg taattgcctt gcggtcagat gacgcgccac cgcctcgttg gccgcgatca
    32821 tgaattcctc gataattcga tgagcgatgt tgcgctctgc gcggatgatg tcctccggca
    32881 ctccttgaat gtcgagaatg atctccgctt ccggtagatc gaagtctagg ttaccgcgag
    32941 ctttgcgttt ttcaaaaatc agcaacgtga gctcttccat cagctcgaat tgagacacga
    33001 gatccgtata gcgttcgatc gttggacgat cgcgatctac caggatccgg cccacgtcgg
    33061 tataggtcat gcgctcccgg ctgcggatca ccgaattgaa gaaacggctg ccaatgactt
    33121 cgccgttgaa gctgtattca atgcacgccg tttttgtaag ccggtcctcg cggggattga
    33181 gactgcagat gccgttggat agcgcctccg ggagcatagg gatggcgcgg tcgggaaaat
    33241 aaacgctggt ggcgcggctg taagcctcct ggtccagcgc ggtggcggtg ctgacataat
    33301 gcgcgacatc ggcgatacaa acgtagagct catatccgct atcggtcttg cgcacgtaaa
    33361 cggcatcatc gaagtcgcgt gcgcgctcgc catcgattgt gacgatcggc aaatgccgca
    33421 ggtcggtgcg ggaggtgatt tcatcgggac tgacagagaa agacgcgctg cttgtctccc
    33481 ggtgaacctc gggcggaaag ctgctgctca gcccgtaacg gaaaacaatc gattgcacct
    33541 gcacttcggg atcatccggg ttgccgatga ccttcaggag ctcggcctgg ggcggcgatg
    33601 tcgcagttcc ataacgagaa atctcagtag caatgatttg gcccttctct ggccgttcat
    33661 gtccgcgggt taacgggata accggtgcga ttcttggatc catggggact aaatagccat
    33721 gaccctcgat ttcatcgtaa gttccgatca gacgcttgtg accgcgttcg agcacctgca
    33781 taatatgagt ttccaagccg ccgcgtttgt tccgatcgac acggaccatg acgcgatcgc
    33841 catgcataac acgacgcatc tcgcgccgat tcaagtagag atccacagcg ctcttgtcat
    33901 cgaggatgag gaagccgtag ccgtccggat gggcatggac tctgccggtc attaggttat
    33961 cgctatcggg taagccgtaa tgattctttt ttagccggac cattcttcct tcgcggacca
    34021 gccgccgtaa ggaatcgatg acgatttgga ggtcatgctg tttgaacccg ccctttcgag
    34081 cgatttcgac cggggttaaa cgaaggcgcg gctttccttt gaatagatcg cagatctttt
    34141 ctcgtgcctg cggtgattcc gtcgtagccg ttgcagattt acgcgccata taaatgaaac
    34201 atcttatgga atcgggcgat ttgcaatcaa ctgcggcgct gcgatcaggt ccgcgatgga
    34261 tcgatcgcaa gctcggtcat caaccagtca tcgcccaagt caggcggcgg cggaatttca
    34321 gtcaaggtta tttcctgctg cagtgatttt attattgtcc gggcaacctc gataaaatct
    34381 gcaaccgcta gaagcacgat catagccttc ttgcggtcga tcgtgcggat gatgccgact
    34441 tcttcatagg actctagaac gaatttgaca taggcgatat ctcgagggca gatctccaag
    34501 tagatgggaa ccaagctcat aatttaccga ctcagaaatt gctcgagctc tgccggtagt
    34561 gattctttct gttcttgcgc atccgatcgt ttgtcaatgg caggtggttc gatggttttc
    34621 ggtaaagatc cgcgccggat gatctgggcg atgatccggg tgtccggccg aactcgaaga
    34681 ttggccggag tgccttcgac gcggaagggc aaattgattt gaccgctccg gtctaggaga
    34741 taacgcagac ttttgttctc gcgcagcagc tcctggctga ttcggactga catgaccaac
    34801 attccattcc atcgggtcgc ctgatcggtc atcatccatc ccgcggccat gattttataa
    34861 tctgcggttg taagctgtaa attctcggtg cggatcactt cttggtcgag tatgactagc
    34921 gcctcgaggt tttcgaaagg agtgtctttg gatctcccca actcggctaa gctgggcgag
    34981 agcggaagcg agatggctga cccagaactg ccgatccgag acatcatcag cgcgatcagg
    35041 ttaaagtcct tgagagttcc gcccggaacc cgggtttcgc cggcgccttc taacgagccc
    35101 ggcttcatcg cgccgttgtg cgcctcggca ctgaattctc cttggaagtt caggtgaccc
    35161 tctatgcggt ctttgaacga ggggaatttg cttatgagga gagacggtag atccactgaa
    35221 ttcaactgcg cataaagttc caggcgttga gatttgtcgg tcccggtgat cgatacatcg
    35281 cggctgcgcc agctcccgtt taaagcgcga aaagaaaatt ctctgagact tatctcttct
    35341 gccgaccaga ctatctggct acgcaggtcg agatagggca gatcttgcag tgtcccgtcc
    35401 ggcgatgaca ccgtgccccg cagccacggt gtgccgtttt ccaatgtgag cttgcccctc
    35461 aatctgacgt tttgcaggcg atccgatttc gccactggga attcggcaag atcgactaga
    35521 tgtaaatttg ccgagcgaag ctcgcacgtt agcgcgggaa ccgccaggtc agccactgct
    35581 cctatcatct cgatgtcaga tgaccggacc cgaaacgcgg cattttcgat gcgagcttct
    35641 ttgccaagaa acaagatctt ggcccttagc cgttcgatct tgcgcctgct gctctcatgt
    35701 cttaactccg cttcattgat ttcaagcgcg ccattcatat tccaggagga ctcgccgatc
    35761 atcacttttg aggcgacaat ctcgccatgt aagttcccgg tcgttcgaaa tgcctgcaag
    35821 gccggaaaga gctcgatcac ctcggataaa tcggaccggt cgctgcgcgc tctgagttgt
    35881 agtcgaggcc gtgatccacg ctcataaacc cccgagacgt tgatcttggt gctgcccact
    35941 gtcagaagcg attcatgaat cacgaatcgg ttgtcttgcc gtgaaatttt tgaggaccat
    36001 tcagcaatgg tccctcgtgg cttgcgcagc caagtgccgt agcgaatctc actgtttcgg
    36061 gcccgcacta tcgctcctaa gcgcaactta tcccatgttc cttcgatgac actatcgaag
    36121 ctgacctcac cttcgccgct gagctcaatt ggcaaaacat cctgaaacac gcgcgtgcct
    36181 tgcagacgag tgatatcgat ggaactcaga cgaagattgc ccttcaattg agcttcggac
    36241 caaagtccgt tcgtcgaaag atccgccgag ccgttcagga cggcgttata atctgaagag
    36301 ccgaggatcg gcgctttgac gatgaagtct acaatctgag gccgggcgag aacgccggca
    36361 agtttcgcgc gcaaggacaa gggcccggcg atatcgagct ccggagggat tttatctcgg
    36421 aaaaacggaa ccgcccgggt gagtaggggc acgaataacg agtcgaattc tacattgact
    36481 tcgaccggtt gttctttcca tcctttgccg cgaacgaagg gtcctaaata tcccgctact
    36541 cgcacgtctt ggtccaaatc ctcacttaac gacgcggcca agctcatttt cgctcgaccg
    36601 ttggggttga ttccttggag gtctatgtcg acccgcttga tctgcatttc cgccggcgcc
    36661 cgtaccgaac gatcgataaa gtcaatgcgc ccgttcttta tttgaaaatt ggtaacgacg
    36721 aaattgattg agctatgctg cctctttgaa gccgacgagc gtgcgttggg cgtttccacg
    36781 agctgtttgc gccggtcctc caaggaagaa atattcatca gaccttcttc gttggtgatg
    36841 atctgaaatt ccggttcgac aaaggttaat ttgttgatga caatcctgcc gaacatcagt
    36901 gagagcaggc tgacacccat cctgagttcc tgtgctcgta acaatggagt tgcggcaaaa
    36961 attggacggt cgccgattcg gaagttcttg gcggaaaaac cgagcccggt gagcaaggtc
    37021 atttcgaatt cgtcgaaagc gacgtctttg ccggcgaccc tttggagttc ctcatagacg
    37081 agatcgcgtc tgatagcagc gagaaggttg acggagtaga cgagcgcaag ggctgcgatg
    37141 atgaccgtgc ccacggcgaa tgaaatggat tttttggaca atctcatgcg cccgagggat
    37201 gaaccagaac tctcaatttg cagcagcggt gtttagcgag aggtttttcg gctggtcgcg
    37261 cccctctgac tgccgcccca ttttggcagg caatcagttg acccgttcta caatggtcgc
    37321 gatgccttga ccgacgccga tgcacatggt tgccagacca tagcggctgc cgcgtctctt
    37381 catctcgtga accaatgtgg tcataatgcg cgcgccgctg catccgagag gatgtccgag
    37441 agcgatggct ccaccattga cgttcaactt gctcagatcg atgccgagct cgcgcgcgca
    37501 ggcgagcacc tgtgaagcga aggcctcgtt gatttccact aagtctattt gatcaatggt
    37561 gagcccggcg cgtttcatgg ctttctgagc ggctgggatc ggcccgatgc ccatataatt
    37621 aggatgaacg ccggccgcgg ccgaggcaat aatacgcgcc ataggtttga gccgcagctg
    37681 ctcggctttt tccggtgtgg caatcagcag ggccgctgcc ccatcggaga gcggcgacga
    37741 gttgcctgcc gttaccgaac cgccttttct aaaggccggc tgcaaagccg cgagcttttc
    37801 cagtgaagtg tcggcgcgag gtccttcgtc tttgtcatgc accagcggcg cgccttttct
    37861 ctgtggaatc tcgaccgcaa tgatctcgtc tcgaagcact cctcgatctt gtgcttgtaa
    37921 agctttctta tgactggata atgcaaatgc gtcttggtct tcgcgactga tatggtattt
    37981 gtcggcaaca ttctcagcgg tctcgccatt attgatcagc gagtagagct cgcccatgcg
    38041 cttgttcggg aatcgccagc ccaaggagct atcgtacata gtcacatcac cgcgctggaa
    38101 ggaagtgttg ggctttccca tcacccacgg gccgcgggtc atactttcga caccgccggc
    38161 gacgaacaga tccccatggt cggtttccac cgcgcgccct gcttgattga tcgcttccaa
    38221 gcccgagccg cataagcgat taaccgtggc gcccggaatg gaatagggaa ggcccgcgag
    38281 taacgaagca ttacgggcca cattgcgtga gtcctcgccg gcttgattgg tgcagccgaa
    38341 gtaaacgtct tcaatctctt ccttattgac gccagttctc tcaacgagct tggcaatgat
    38401 gtaggcgccg aggtcgtcgg tgcgaatgtc tttaaggatg ccgctgtgtc gccccatggg
    38461 cgtgcgtacg gcctcgacaa tgatggcttc tttcatgatt tcacctgttc ttcataagag
    38521 tagaaccctc tgccggtggt ttcgccatgg aaaccagcca taaccagttt cttaatgagc
    38581 ggcgcggggc gataacgatc gtcgccggtt tcgcgctcga ggccttcgag gacggcgaga
    38641 acttcatcta gaccaatctg gtccgcccaa cgcaatggcc ccattggata attgacaccc
    38701 aggcgcatcg caatgtcgat ttcatcagct tcagcaaccc cttcttcgag gctgcgcgcc
    38761 gcctcattga taatcaaact cagaatgcgg gggaaagtta gaccggcggc atccttcact
    38821 ttaacgaatt ctttacctag cgacctaaaa tgatcttgcg cttgctgcag cgcgctgtcg
    38881 gaggttcgca gtcccgccgc gatctctatg atttttctgt ccgcaagtgg gtaaaaagtc
    38941 gcaaagccga ttagccgttc cggccgtttt gtccaagacg ccattagagt ggttgagccg
    39001 accagacagg acgtgatgat cactgcagac ggcgttaaac cgcggtcgag cttttctagc
    39061 gtacgccttt tcattgcctc gtcgccggct gaagtatcga tcaccagcgc tgcgtcggct
    39121 tgaatttgtc cggcatcgct caccaactct gcatcgagac ctttggcttt gctgagcgca
    39181 atccagtcct gcgccagccg gttggtgccg acgatcgcta agctactttt tgtcgtagac
    39241 atagaatcct cggccggtct tgcggcccaa gttgccgccc attaccatgc gctgttggat
    39301 cgggctcgga cgaaatcggg gatcgtggaa aaatgattga tagagcgagt cagtagctgc
    39361 gaaattgatg tcattgccga taagatccat gagctcgaaa ggtcccatgc ggaaattgcc
    39421 ggcttccttc atcacacgat cgataatctc caccgagccg tcgccgtccc caaggatgcg
    39481 caatgcctca ttgtaaaacg gccgggcgat ccgattgaca ataaagcccg gcgtgtcttt
    39541 agcgcgcgcc ggcgttttcc ccatgcggcg catcaaatca gtggccttat cgatgctagc
    39601 cgggctagtc cgatccccct gcacaacttc gaccagcgcc atgagcggtg gcggattaaa
    39661 aaaatgcagg cccagcactc gctcctgatg ctgcgtgttt gccgcaatcg ccgtgatgtt
    39721 aaacgaagat gtgttgcttg ccagaagagt ttcccgaccg caaatggaat cgagctgctt
    39781 gaagatatcg cgcttgagtt ccaatttttc cggcgcggcc tcaatcacca gctcgcttcc
    39841 ttgacacgcc tcaagctggg tggtgctgcg caaccgagcg acaatctcct gctcctgctc
    39901 gacagaaagc tttcctcgct cccggctgcc ctttaaaaat ccgcgaatac gaccaaggcc
    39961 ggtatcgata aattcctgct tgatgtcata cagcagagtg tcaaatccgg cctgtgccgc
    40021 aacctgggca atgcccgccc ccatggtgcc ggcgccgagc actgcgattt tttctattgg
    40081 attggtcatg cgtttggtaa ttcagttgcc gggttgcctg ctcatagtcg caacggaatt
    40141 tattttccgg taaaactggg agcgcgtttt tctaagaacg cgcgtacgcc ctcatcgtaa
    40201 tccgagcttc gcccggcgat ttcctgcaaa caggcttcgt actcgagagc cgcttcgaga
    40261 cttgccaaat tggcgtgatt caccgctcgc ttgatcaagc cgatgccgcg cggcgcgccc
    40321 gcgactctct cggccaattt cctggctgcg tcggaaaact cagcgctcgg aaatactttc
    40381 gccaccagcc cgattcgttg tgcctcccga gcatcgactg tctcgcccaa cagcagcagc
    40441 tccatcgctt tgctcaggcc gaccaaccgg ggaaggatga aactgccgcc ggaatccggc
    40501 gcgaggccga cacggacgaa ggattggatg aatttcgcgt cttccgacgc cacgcgcaga
    40561 tcgcaggcca aagcgaagtt acagcccgcc ccggccgcga ccccgttcac agcggcaatg
    40621 acgatctttt ccatctgccg aatttgccgg atcatcggat tatatttttc ccgcaaggag
    40681 gcgccaagtg atcccttctg atccggtgtt cgtcccttca gatcttgtcc ggcgcagaaa
    40741 gcacggccag ctcccgtaat catgaaacaa cgcacctctt ggtcacgttc gccgactttg
    40801 agcgcgtcca tgacttcctg gttcatctgc ggcacaaagg cattaagcgc ttgcggacga
    40861 ttgagcgtaa tcgtcgcgac gccgctttcc ttcttgtaga tgatagtttc gtaagccaat
    40921 tgagctgagt cgccttatct gcctttgtat tcagctttgc gtttttccag aaacgccttc
    40981 atgccttctt tgcggtcttc ggatgcgagc agcatataaa aagattttcg ttcgaagtcg
    41041 agtccttcgt caagcgcggt gttagcggct ttgagcaccg cctcttttgc cgcttgaatc
    41101 gccagtttcg gcttcacggc gattttcttg gcgatattct tggcttcttc gagcatcagt
    41161 tcgacgggaa ccacccggtt aaccaagccc aagcgttcag cttcggcggc cccgatgatc
    41221 tccccggtga ggaccagctc catggcgcgg tgcttgccaa tcaaatgcgt caaccgctgc
    41281 gtgccgccgc ttcccgggat gatcgccaga tttacttccg gttggccaaa acgcgccgtt
    41341 tccgaagcga cgatgatgtc gcagctcatc gccaactcac aaccgccgcc cagagcgaag
    41401 ccgctgaccg cggcaatgac cggcttggaa atttgattga tcttatcccg gtatgcgaga
    41461 cgcccttgca cccgctcgtc gaacgcgccg agatccgcca tctcctttat gtctgcgccg
    41521 gcggcgaata ctttctctcc accggtgacg atgatgactc gaataccttg atcttgatcc
    41581 agggcttgca tcgccaaaga caagtctttg accaaatcat aggagagcga gttgagctgc
    41641 tgaggccggt tcaaacttac gacggcaatg gcgccgtcga tttcaacgac aacgttctcg
    41701 tagtccataa tcggcttgaa gtttaagaca tacaccatgg ccggagtcga ttcaacaaac
    41761 gctttatctc gctaccgctc ggaacagctg cgaaaacacg cttttcagaa ggcaatcggt
    41821 tggctgtgcc aaatgccacc gaagagaaat ccttgtgcct ccggagagaa gtgttgccgc
    41881 ccatcaatga tgatgaaggg gggtgcgtac gggttatcgc tcaccaagct caattgacac
    41941 gtctacggcg atttgcaact atcgttttct tatgcccagc cgcttggaac ctgctacccg
    42001 ccactacgat gaaaaacttc aacatatcct aaaaacctca gccaaaatct ttgccgaaaa
    42061 gggctttcat cgcacctcgg tgcgcgacat ctcgcgggcg acaaaaatga gcttggcggg
    42121 tctgtattat tactttacca ccaaggaaga gctcctttat ctgatccagg agcgctgttt
    42181 cgtaacgctg ttgcatcgtt gggaacaagc tgcgggcgcc gagatggatg tgcgcgctcg
    42241 catcggactg ttcgtcgcaa atcacctgag ctttttttgg cataacacgc atgagatgaa
    42301 ggtgatggcg catgaagacg aatcattgaa cggcgacttc gccgataaga ttctggtgct
    42361 caagcggcgc tatgtgaaaa ttcttatgga tttggttgag gaatggcagc agcaagacgg
    42421 cggcaaagga gtggacttgc gggtcgcgac cttttctctt ttcggcatga tgaactggac
    42481 ctatacatgg taccaggcca agcgcgattt accgctgcct cagctcatcg agcagatggt
    42541 gcgtattttc ttctttggag tgttaaaagc aggacagatc gatgagcgtt ggtttatttc
    42601 gaacaccagt cgagagaaaa atgccttttc actttggcac aacgtccctt agacatgata
    42661 gatgcgagga gcaaaaatga gtctagcgag agtcggcgat aaagcgccag atttcgccgt
    42721 taaaggaatg caccagaaga gagtctccga gtactccttg tctcgcttta agggcaaatg
    42781 gctcgtgtta tttttctacc cggccgattt cacctttata tgtccaacgg aggtcgtcgg
    42841 cttcagcaag ctggccaagg aatttgccgc ccaagacgcc gaaattctcg gcgtcagcgt
    42901 cgattcgttg gacagccatc gttcctgggc ggaggagttg ggcggcatcg attatccgct
    42961 gcttagcgat gaggacaagt cgttgagccg agcctatggc gttctcgatg aaagggaggg
    43021 agtgccgtta agagctacct tcatcatcaa tcccaacggc gaagtcagtt acctggttat
    43081 cagtcacgta aatgtcgggc gcagcgtcga ggaaactctg cgcgttgtta aagcgctccg
    43141 caccgaacgc ctttgcccgt cgggatggaa acccggcgag cccaccggcg gtctcgacca
    43201 caaatattga gctggcttcc aactaaatat ctgtgcctgc tcctgcccgc tgtctgctag
    43261 tatgagtatt tcaattttga agcggtgcta attcgtcaat ggccctggaa gttatacagc
    43321 agaagaaacg attcattgaa agccgggcgc gcatccgtga atttgttttc ggcatacaag
    43381 acggactgat cagcacggtt ggcctcctgg ccggtattca aggggccact gagaatcgct
    43441 tggtggtcat catagcgggt ttcaccgcga tgttttcggg cgcgatctca atggccgccg
    43501 gctcctatct ttcgtccgcg gcgcagaagg agatattcga caaggagttg cgcgacgccg
    43561 aagcgctggc tgagcgggag ccctatgttg cggccgaggg gctcctgagg gcgctgggcg
    43621 aagagggtct gagtaaggaa cagagctacc ggatggtcag agtcctcagc caagagcgta
    43681 atgtgttcat gcggaccttc caggaaaaag tcttcggctt gggcagcgct gaaatcaatc
    43741 agccagtgca ggctgcgcta gtgatggggt tctcatttat tgtcggcgcg atcgtgccga
    43801 ttattccgta catgatcacc tcgggcctga cggcttttta tctttcggct ctgcttggcg
    43861 gcataacgct ttttggcgtc ggcgctttta aggcgaaact cgcgggcaag tcactggcac
    43921 tcggcggttt agaatttttc ctcgttgccg tcggggcggc gggagcggga tttctgatcg
    43981 gcttggttgt gcagtacttc tttccgggag tcgatattcc tcccgcctaa tcctgcctca
    44041 acgttcaccg taagtggttc ctgcacctga gcgaagatag aatccaattt taacggcctt
    44101 tagggtgtcg gaccagagct gatacctttg ccgggacttc agtgcaccaa ccggggagat
    44161 gcaaatctct agacggggcc gctgggttgc cttcgctccg acgcgctcgc caatagcgca
    44221 gtgtcggttt gaacgaccga actcggcata aacaggctgc gcagcaaacc taagttttca
    44281 cggtcttccg ccaagtccag ccctttgggt gtttcgagac acatcggtac accccaaaat
    44341 cgtttatcgt tcattagcaa tcgaaacgcc tccaagccga tgtgcccttt gccgatgtgc
    44401 tcgtgccggt cgacccgcga gttaaattcc tttttcgaat cgttgaggtg aaacgccgct
    44461 agcctctcga tgccgatggt ctcgtcgaat tctgtaaatg ttcgttcgta accctctttc
    44521 gagcggatat cgtagcctgc ggcaaaagca tgctctgtat cgaaacacac gcgcaagcgg
    44581 tcgctttccc gggtggcatc gaacattttg acgatgtgct cgaagcgaaa gccgagattt
    44641 gatccctggc ccgctgtgat ctccagggtc actttcacgc gatagccagg acacgcctgg
    44701 tgaatctcat tgagagaact cgcgatcgtc ttaatgcctt ggtcttctcc cgcgccgaca
    44761 tgcgagccgg g
//
LOCUS       DASUVS010000110        10811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1783793, whole genome shotgun sequence.
ACCESSION   DASUVS010000110 DASUVS010000000
VERSION     DASUVS010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10811
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783793"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..855)
                     /locus_tag="VF182_06230"
     CDS             complement(226..855)
                     /locus_tag="VF182_06230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_1061 family FMN-dependent PPOX-type
                     flavoprotein"
                     /protein_id="HEX6436708.1"
                     /translation="MEMINRNDAYTVTSLDDLAKIYAKPLPNVATKETDHITEVGRAF
                     IAASPFLVIGTSNGSSIDCSPKGDAPGFVQLLDDRTLLIPDRPGNNRIDGMKNLLVNP
                     KVGIIFMIPGTNATYRVTGSAKISTDPELLKRFEVGGKPPRVVLVVAVEEAFNHCPKA
                     LVRAKLWEMERPSGAPTHGDFAAARDGKDAAYARQYNEKYAERMKTELY"
     gene            complement(1097..1585)
                     /locus_tag="VF182_06235"
     CDS             complement(1097..1585)
                     /locus_tag="VF182_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436709.1"
                     /translation="MKYRFVIVVFVLSVAAFLLSSTSASTRDAGAQGGCLPGTYLVVE
                     GSGTQSLWTLSQDGGFQITSSAERAFGFSHIQGVWKKTGARDAKAKGLDFNFKETPID
                     GGVPPSQITRLDIVMRFAERCEEIDGSFELRAFNPNDDPLDSNSPAIASDTFFGRRVR
                     ID"
     gene            1949..2428
                     /locus_tag="VF182_06240"
     CDS             1949..2428
                     /locus_tag="VF182_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436710.1"
                     /translation="MTSQVRAALLSFILTLNGILTVTPYADADGGCFQGTYFVRESSG
                     TQSLWTFSQDGTYQSASSAEAARSFGHIQGAWEKTRDRELRSTGLAFTFSGGNGDVGV
                     PPLRTARVDAAMSFTHKCEEVEGTFEVRFFGPDDDPVSEPGEFVFSDTFTGRRVTVN"
     gene            2487..2972
                     /locus_tag="VF182_06245"
     CDS             2487..2972
                     /locus_tag="VF182_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436711.1"
                     /translation="MLAKLRFSTIKTGLVLFWAIWLTLVTLTNFADAMRQLDVLPSDF
                     KLASYNFDLVQKTVGAHGIPTSVAGVLFAGVILWELLGSLLFWRAWRVMWRHGNGAAA
                     EVAQAFAVSLALWAAFLIATEALVNYETAATHKTTLIAQLASLLVVRASTSPTKSGQA
                     D"
     gene            complement(3154..4104)
                     /locus_tag="VF182_06250"
     CDS             complement(3154..4104)
                     /locus_tag="VF182_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011320060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX6436712.1"
                     /translation="MKAIYWDGREVTLDSSYPPPVPDQETAIVKVHLAGICSTDLQIF
                     KGYLNFRGVPGHEFVGSVQHGPEKWLGKRVVGEINFGCGHCECCARDLTRHCPNRTVM
                     GILNADGAFAEAVAVRLENLHLVPDSVNDEQAVFTEPLAAAFEILQQIQINTGDEILV
                     LGDGKLGNLCAQVLRLTGAKLIALGKHQDKLELIRRTGVRTMLLNDWKPRLFDVVVEA
                     TGSAAGLELAISAVRPRGTLVLKSTIAGAHQVSLAPVVINEINLIGSRCGVFPDALEA
                     LAASQVTVLPLIEQVYPLEQGIEAIQHAGRRGARKILLRP"
     gene            complement(4117..4932)
                     /locus_tag="VF182_06255"
     CDS             complement(4117..4932)
                     /locus_tag="VF182_06255"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX6436713.1"
                     /translation="MKSNVTQQRVPVHGDGGHWVHVSESPRHVRIIFNRETIADSKHA
                     KLVREAEVLPVYYFPREDVRTELLSPSQRRTRCPYKGEASFWSIDLGGKHAEDIGWSY
                     QDPLSAADAIRNHFAFEWNKMDHWLEEDEEIFVHARDPYKRVDVLPSSRHIRVVIDGQ
                     LVAETRRPRLLFETNHPVRYYIPQEDVRMELLIPSATKSRCPYKGPADYWSVKLGEQQ
                     FDDMVWGYMEPIAECPKIKGLLCFFHERGADIMVDGEEVPRPKTKWARELKKS"
     gene            complement(4941..5894)
                     /locus_tag="VF182_06260"
     CDS             complement(4941..5894)
                     /locus_tag="VF182_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEX6436714.1"
                     /translation="MSVILGTGEFRYEVTECWGKLPDGWTFHEVASVGVDRNDNVYCF
                     TRGAHPVIVFDRDGNFLRSWGEGKFKRAHGVTMGPDDTIYLTDDGDHTVRKCTLDGKI
                     LLTLGIPGKPAPYQSGEPFNRCTHVALSPKEEIYVSDGYGNSRVHKYSPDGKLLLSWG
                     ESGSDPGQFNIVHNICTDRDGWIYVADRENNRVQIFDANGKYETQWNNMHRPCALYMD
                     GAREDPLCYVGELGPGYSVNKDAPNLGHRVDIYNKAGNRVARLGDVKAGEAPGQFIAP
                     HGIALDSRGDIYVGEVSWTIMGQHLKPPRELRCLQKLAKLS"
     gene            complement(5937..6251)
                     /gene="glpE"
                     /locus_tag="VF182_06265"
     CDS             complement(5937..6251)
                     /gene="glpE"
                     /locus_tag="VF182_06265"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019084097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HEX6436715.1"
                     /translation="MEIPQIEIHQAKERLDQENAVFVDIRDPGSYQAAHIPGALHLHD
                     GNVQGFVQNTDKEKPVVVYCYHGNSSLGAAAYLIENGFKNVASMSGGFEAWRQVYAHE
                     PG"
     gene            6370..7575
                     /locus_tag="VF182_06270"
     CDS             6370..7575
                     /locus_tag="VF182_06270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6436716.1"
                     /translation="MKLSPLTFFSLAAVCSIAGSSGAAMLAPFSMSARGYSLALAGIP
                     LVVNGAGRVCSDLSSGVLASYFSSGMLIIAASLFALLISSASILFLDATAVFLVAFTL
                     LGITEAMFALSIRKIAFEKSEPGQQGRAQGQVASALGIGFAVGPLLGGFAGKKFGPDA
                     LFVVYAAPQLLSLLFLLLGGAHRSGKITQEPSRRILREGQKLLSSPSFLASCLAIFQS
                     FLFLVGVTRVAFPFLAANQRGLGLDWVGTMVSISRLADTAGRYSGGWLCDRIRPERVI
                     LVGILIGIPMFVLQVYGSGVTTLLIPLCVMTMGFGYTNVGATMFALQSAGSAGKGLAL
                     GLSRASTSMGQMLGPLVAGLLVEKFGYEHGFETMALISGVVLFVVWRGLSTQPAGAAT
                     DAGRSTDSG"
     gene            complement(7588..8379)
                     /locus_tag="VF182_06275"
     CDS             complement(7588..8379)
                     /locus_tag="VF182_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX6436717.1"
                     /translation="MAERVWERFLTEQDKATLVGRPERKIGFGERPALLLIDLYRWVF
                     GDKPEPVVEAIKTWPGSCGLAGWNALPHIQKLLGKAREVGIPVIHVTGMDGVAIEPWS
                     FRRDKTKRADMNQEQLDRNRRKFDIVEQVKPIPGETVLKKVSPSAFWGTPLIAHLNFL
                     GVDTVLTCGESTSGCVRASVVDGTTNRLRMIVAEECVFDRHEACHAINLFDMNQKYAD
                     VLPLDEILKYLDAWRAEKAGQVGYANDSVAYLKDLALGEDFKGLR"
     gene            8959..9924
                     /locus_tag="VF182_06280"
     CDS             8959..9924
                     /locus_tag="VF182_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX6436718.1"
                     /translation="MQSITLSILIWLVLVSPARASYLTAAEQLKDIGASFSWQDVRSR
                     SLKIVVEYQMNGESRRAHLGSGFLISGDGLFVTAYHVMSYCLLSQKGKSRFAESVDCS
                     IANPRARYKAQNGDQEYEIQILSHLTEEDSTNGKKQHTPDEIIKNRDFVIGRLKADPK
                     IHFSHWELRDYEQGKISLSKPRADFELKPLMPPRKVFIVGYPGDGEFEIAHGFLNLTD
                     EKHRGYFAADYKLYEPAYLKTEGIEPDTKWGIAVANHMSGGVVVDAKGHPVGVVVNGN
                     QNTAGVLSIENVLETFFSRKRSSESQPKVFLAPNSTPLFLKRAPD"
     gene            10089..10523
                     /locus_tag="VF182_06285"
     CDS             10089..10523
                     /locus_tag="VF182_06285"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX6436719.1"
                     /translation="MLLRMGASVFLGAAIGFERERIRRAAGLRTHLLVALASATFMLV
                     STQFVYYQHYSKDDLVGVDSSRIAASVVTGVGFLGAGAILRTGLSVQGLTTAASLWLV
                     SAVGLASGAGMFAEAGTATIVTLFSLVILRRFEGKTKGNGQG"
BASE COUNT         2296 a         3047 c         2867 g         2601 t
ORIGIN      
        1 gtcttgtaga agtctggtcg gggttcaggg tgcagccgca gagtaccgct cgttcatttg
       61 aaatcgatta gacggcgggc cttctctaac gagtaacaaa gatctgatcg agcaattctc
      121 ggcacgcaac gattctacag acgagccctc tgcctttaat tataaccggc ttgtgccctt
      181 ttgttcccgc gttcgtttac tgtgtctgct cgtttcacag cccggtcaat aaagctcggt
      241 cttcatgcgc tcggcatatt tctcgttata ctgcctggcg taggcggcat ccttgccgtc
      301 gcgcgcggcg gcgaagtcgc cgtgggtcgg ggcgccgctc ggccgctcca tctcccacag
      361 cttggcccgc accagggctt tgggacagtg attgaaggct tcctcgacag cgacgaccag
      421 gaccacacgt ggcggcttgc cacctacttc gaatcgcttc agcaactccg gatccgtgct
      481 gatcttggcg ctaccagtca cgcggtacgt tgcattggtt cccggaatca taaaaatgat
      541 accgactttc ggattgacga gcaggttctt catcccgtcg atgcggttgt tgcccggccg
      601 gtcgggaatc agcagtgtgc ggtcatcgag cagctgcaca aatcccggcg catcgccctt
      661 gggcgaacag tcgatgctgc tcccgttcga ggtcccgatc accaggaatg gcgatgccgc
      721 gatgaaggcg cgcccgacct ccgtgatgtg gtcggtttcc ttggtggcga cgttgggcaa
      781 cggcttggca taaatcttcg cgagatcgtc gagtgacgta acggtatagg catcgttccg
      841 gtttatcatc tccatgtcat attcctcctg ggtcgtgcac gctagcagtg ttcttcgtta
      901 ggtctctgca atatcgactg ctaaccaagt cttgtcaata ttgcaacgtg tggcttaggc
      961 ccacgggata gcggtcgtct cctaacgaac ggcggcttat ttcgatcagt gagtttagtt
     1021 cggcgtaaaa gtagctgtag ttttttcgtt tgtggtattg agtcgctccc aacgcgctgc
     1081 acgctactcg accatcctaa tcaatacgta cccttcgccc aaagaaggta tcagaagcaa
     1141 tggccggact attggaatcc aagggatcgt cattgggatt gaaagcgcgc aactcgaagc
     1201 tcccatcgat ttcttcacac ctttcagcga acctcatgac aatatcgagt ctggtgatct
     1261 gcgaaggtgg aacaccaccg tcgatcggag tctctttaaa gttgaagtcc aggcccttcg
     1321 cttttgcgtc ccgagcgccg gttttcttcc aaacaccttg tatgtggctg aagccaaacg
     1381 ccctttcggc agagctcgta atctgaaaac caccgtcctg tgacaatgtc cagaggcttt
     1441 gagtgccact gccttcaacg acaagataag tgccgggcaa acagccgccc tgtgctcccg
     1501 catcgcgcgt gctagcgctg gtcgaactca ataagaaagc cgcgaccgac aagacaaaga
     1561 ccacgattac aaaccggtat ttcatggtgc actccccgct gcggttattc gtgcagactt
     1621 ctcggaacac cggcttctag cacaggatgc cccatcaaac aaatccggac agcgttcgga
     1681 ttgaggagcg agacgcgtcc atggaccgta ggattgccca atgacctatc aggacgaaca
     1741 gaacccgaat ccggaaacaa ccgcgggcga aagtggtatt acccgagcct gatcacaatg
     1801 ctcatcggag agaagtgctt gacagatttt tttcaaaaaa gggatattac aaccggccac
     1861 taaactcttg acgtgtttgc ccaatttatt gatcttacga caacaaacaa ctgtcggacg
     1921 gagaagagca actatcatag gaggttttat gacatcgcag gttcgtgcgg ctttgttatc
     1981 ctttatcctg acactgaatg gcattctgac cgtaacccct tacgccgacg ctgatggcgg
     2041 ctgctttcag ggaacctact ttgtcagaga gagcagcggc acgcagagct tatggacgtt
     2101 ttcacaagat ggtacttatc aaagcgccag ctcggcggaa gcggcccgca gttttggtca
     2161 catacagggt gcctgggaaa agacccggga tcgagaactg cgctcaacag gtttggcttt
     2221 tacttttagt ggcggcaacg gcgacgtagg tgttccgcca cttcggaccg ctcgggttga
     2281 tgccgctatg tcgttcacac ataaatgtga ggaggtggaa ggaacctttg aggttcgctt
     2341 ctttggcccg gacgacgatc ccgtctcgga gcccggtgag ttcgtttttt ccgatacctt
     2401 taccgggcgg cgtgtcacgg ttaattagtt tcgctgcaag tgctaagttc gaggctcgcc
     2461 actgctgctt caccacggcg ttagtgatgc tcgcgaagct ccgattttca actataaaga
     2521 ccggtctggt actcttctgg gcgatctggc tgaccctcgt aacgctcacg aacttcgctg
     2581 atgcgatgag gcagctggac gtgctcccga gtgatttcaa gctcgcctct tacaattttg
     2641 atcttgtgca gaagacggtc ggtgctcacg gaataccgac gagcgtcgcg ggcgtactgt
     2701 tcgccggggt tattttgtgg gagttgctcg gcagcctcct gttctggcgt gcatggcgag
     2761 ttatgtggcg ccacggaaac ggcgcggctg cagaagtcgc gcaggcattt gcagtcagtc
     2821 tcgctctatg ggctgccttt ctgattgcca cggaggcgtt ggtgaactac gagaccgccg
     2881 cgacgcacaa gacaaccctg atcgcgcaac ttgcaagcct cctcgtagtt cgcgccagca
     2941 cttcgcccac aaagtctgga caagcggact agctgacttc agggaagcgt gtgactttcc
     3001 gactacgctc ttctctgcca tcctccgcaa cggtatagat gctcgtcagc tttaattgca
     3061 gtcgcgtggt aatgttcctc ccggaacgct ccgtggcgtc ctttgcgatt gaacgattgg
     3121 aactgcctct caccgctacg gatttgaggc ggactagggc cgaagcaaaa tttttcgcgc
     3181 gccccggcgc ccggcgtgct gaatcgcttc aatgccttgc tcgagcggat aaacttgctc
     3241 gatcaaaggc aagaccgtta cctggctggc ggcgagcgct tccaaagcat caggaaaaac
     3301 accgcagcgg gaacctatca gattgatttc attgatcacc accggcgcca aggacacctg
     3361 atgagctccg gcgatggtgc tcttcaacac cagagtaccg cgcgggcgca ccgcgctgat
     3421 cgccagttcg agcccggcgg cggaaccagt cgcttcgacg acgacatcga aaagccgcgg
     3481 cttccaatcg ttcagcagca tcgtccgcac tccggtccgc ctgatgagct cgagcttatc
     3541 ttggtgttta ccgagtgcga tcagcttcgc tccggtaagg cgcaagacct gggcgcaaag
     3601 attaccgagc ttgccatcgc cgaggaccaa gatctcgtcg ccggtgttaa tttgaatctg
     3661 ttgaagtatt tcaaatgcgg cggcgagcgg ttcggtgaaa acggcttgtt catcattgac
     3721 tgaatccggc accaggtgaa ggttttctaa gcgcaccgcc acagcctcgg caaacgcgcc
     3781 gtcggcattg agaataccca tgacggttcg attcgggcag tggcgggtta gatctcgggc
     3841 gcaacattcg cagtgtccac agccgaagtt gatttcgccc acgaccctct tgcctaacca
     3901 cttttcgggt ccatgctgga cggagccgac gaactcgtgg ccgggcacac cgcgaaaatt
     3961 caggtagcct ttgaagattt gcagatcggt cgaacagata ccggcgagat gaactttgac
     4021 gattgcggtc tcttggtcag gaacaggcgg cgggtaagaa gaatcgaggg ttacttcacg
     4081 accatcccaa tagatcgcct tcatggtgat gcgctctcac gattttttta actcgcgtgc
     4141 ccatttcgtt ttcggccgtg gaacttcctc gccgtcaacc ataatgtcgg cgccgcgctc
     4201 gtgaaagaag cagagcaagc ctttgatctt cggacattcg gcgatcggtt ccatgtaccc
     4261 ccacaccata tcgtcaaatt gctgctcgcc cagtttaacc gaccaataat ctgccggtcc
     4321 tttataaggg cagcgcgact tggtggcgct tggaatcaat agctccatgc ggacgtcttc
     4381 ctgcgggata taataacgaa ccggatgatt ggtttcgaac agaagtcttg gccggcgagt
     4441 ctcggccacg agttgtccat cgatcacgac tcgaatgtgc cgggagctgg gtaacacgtc
     4501 gactcgtttg tacggatcac gggcatgaac aaaaatctct tcatcttcct ccaaccagtg
     4561 gtccatttta ttccattcaa aggcaaagtg attgcggatt gcgtctgccg cggataatgg
     4621 atcttgatag ctccaaccga tatcctccgc gtgttttccg cccaggtcaa ttgaccaaaa
     4681 agaagcctcg cccttgtaag gacaacgggt tctccgctgg ctcggcgata acaactcggt
     4741 gcggacatct tcccggggaa aataataaac cggcaaaacc tcggcttcgc gaacgagctt
     4801 ggcatgtttg ctgtcagcaa tcgtctcgcg gttgaaaata attcggacgt gcctggggct
     4861 ctcgctcacg tggacccagt gtccgccgtc gccatgcacc ggcacacgct gttgggttac
     4921 attgctcttc accctcgctc ctaactgagt ttcgccagct tctgcaaaca acgaagctcc
     4981 cgcggcggtt ttagatgctg ccccataatc gtccaggaaa cctcgccgac atagatatca
     5041 ccgcgcgagt cgagggcaat accgtgcggc gcgatgaatt gaccgggggc ttcgccggct
     5101 ttgacgtcgc cgagccgagc cacgcggttg cctgctttat tatagatatc cacccggtgc
     5161 ccgagattgg gcgcgtcctt attcaccgaa taccccggac ccaattcgcc gacatagcaa
     5221 agcggatctt cgcgagcacc gtccatgtag agagcgcaag gacgatgcat gttgttccac
     5281 tgagtttcgt acttgccgtt ggcgtcgaat atttgaaccc gattattttc gcgatctgcg
     5341 acgtagatcc aaccatctct gtcggtgcag atgttatgaa cgatgttgaa ctgccccggg
     5401 tccgagcccg attctcccca tgacagcaac agtttcccgt cgggtgaata cttgtgaacc
     5461 cgcgagttgc cgtaaccatc ggaaacgtag atctcttcct tgggagacaa cgccacgtgg
     5521 gtgcagcgat tgaaaggctc gccgctttgg tagggcgccg gcttgccggg aatacccagg
     5581 gtcaggagaa tcttgccgtc caaggtgcac ttgcgcaccg tgtgatcacc gtcatccgtc
     5641 agatagatcg tgtcatccgg ccccatggtg acgccatggg cgcgcttgaa tttgccctcg
     5701 ccccaggaac gcagaaaatt gccatcacga tcgaatacga tcaccggatg cgcgccccgc
     5761 gtaaagcagt agacattgtc gttcctatcg acgccgaccg aggcgacttc atgaaaagtc
     5821 cagccgtccg gaagcttgcc ccagcactcg gtgacctcgt agcgaaactc gccggtacct
     5881 aaaataacgc tcatcgatct cgttcctaac cgccgccaat cacgaagggc aaactttcat
     5941 ccaggctcgt gcgcatagac ttgacgccat gcttcaaaac cgccgctcat gctggcgaca
     6001 tttttaaaac cattttcgat caaataggcg gccgcgccga ggctgctgtt accgtgatag
     6061 caataaacca ccaccggctt ttctttgtcg gtattctgaa cgaacccttg cacattaccg
     6121 tcgtgcagat gcagcgctcc cgggatgtgc gccgcctggt atgagcccgg atcgcggata
     6181 tcgacgaaca ccgcattctc ctgatcgagc cgctctttag cttggtgaat ttctatctgt
     6241 ggaatctcca tgtctcttac ctcgcctaac gctgtccttc tatcaaggcc cgtcggcact
     6301 gtccacaacg ttgaaatttt ttggcgttcc atatggccgc cggcaactca gctgctataa
     6361 tccggcgcca tgaaactttc cccgctcacg tttttttctc tcgctgcggt gtgttcgatt
     6421 gcgggttcat ccggcgccgc gatgctcgcg cccttttcca tgtcggcgcg cggctattcg
     6481 cttgcattgg ccgggattcc actggtcgtc aatggcgcgg gtcgcgtctg ctcggattta
     6541 tcatcgggag tgctggcatc gtatttcagt tcgggcatgc tgatcatcgc cgcctctctg
     6601 tttgcgctac tgatcagcag cgctagcatt ttgtttctcg acgccaccgc tgtttttctc
     6661 gtggctttca cacttttagg catcaccgaa gcaatgtttg cgctatcgat ccgcaagatc
     6721 gccttcgaga agtccgaacc aggacaacaa ggccgcgctc aggggcaagt cgcatcggcc
     6781 ctcggtatcg gctttgccgt aggcccgctg ctgggcggat ttgccggaaa aaagttcgga
     6841 cccgacgctt tgttcgtggt gtatgcggca ccgcaacttc taagcttgtt gtttctgctt
     6901 ctcggcggcg cgcatcgatc gggcaagatc actcaggaac cttccaggcg gatcctgcgc
     6961 gaaggtcaga agctgctcag ctctccttcc ttcctggcct cttgcctggc aatctttcag
     7021 tcatttcttt ttctggtggg agttacccgg gtggcctttc ccttcttggc cgccaatcaa
     7081 cgcggcttgg ggcttgattg ggtcggaacc atggtttcaa tttcgcgcct ggccgacacg
     7141 gccgggcgtt acagcggcgg ttggctttgc gatcgcatca ggcccgagcg cgtgattctc
     7201 gtcggtattc tgatcggcat tccgatgttt gtcttgcagg tttacggcag cggagtaaca
     7261 acactcctga ttccattgtg tgtgatgacc atgggattcg gctacaccaa cgtcggcgcc
     7321 accatgtttg cgttgcaatc cgccggcagc gccggcaaag gactcgccct agggcttagc
     7381 cgcgcgtcga cttcgatggg acagatgctg gggccgttgg tagcgggact cttagtggag
     7441 aagtttggct atgagcatgg attcgaaacc atggcgctca tttccggggt ggtgctgttc
     7501 gtcgtctggc gcgggctgag cacacaaccg gctggcgccg ctaccgacgc cggccggtca
     7561 actgattcag gatagatcgg atccgttcta acgcaatcct ttgaagtctt cgcccaaggc
     7621 aagatctttc aaataagcga cgctgtcatt ggcatagcct acctgtccgg ccttctcagc
     7681 gcgccaggca tcgaggtatt tgagaatctc gtccagcggc aacacgtcgg cgtacttctg
     7741 gttcatgtcg aataggttta tagcgtgaca agcctcgtgc cggtcaaaga cgcattcttc
     7801 ggcaacaatc atgcgcaacc gattggtcgt gccatcgaca acactggcgc gcacacaacc
     7861 gctggtgctt tcgccgcatg tgagcactgt gtcgacccct aggaaattca ggtgcgcgat
     7921 cagcggcgtc ccccagaaag cgctgggcga cacttttttc aacaccgtct ctcccgggat
     7981 cggctttacc tgctcaacga tatcgaattt ccgccggtta cgatcaagct gctcctgatt
     8041 catgtcagct cttttcgtct tatcgcggcg aaacgaccag ggttcgatgg cgacaccgtc
     8101 catcccggtg acgtggataa ccggtatgcc gacttcgcgg gctttgccaa gcagcttctg
     8161 aatgtgcggc aaggcattcc atcctgccag gccgcagctt cccggccagg ttttgatcgc
     8221 ttctaccacg ggttccggct tatcgccgaa aacccaccgg taaagatcga tcagcagcaa
     8281 cgcgggcctt tcgccgaagc cgattttgcg ttcaggcctt cccaccaacg tggctttatc
     8341 ttgttcggtt aaaaaacgct cccatactcg ctcagccata gaaaactcct ttgctggtta
     8401 agtgtttggt catctcatcg gtgcggtagt gactggcaac ctatcactca gttcgtgaga
     8461 ccgtcaagaa acctatgatc accaaatcga cgggtgcatt ctctaacctt accttaaccg
     8521 ctcgctgtag gtagattgag ttggccctta ggtcctttga aggcaatgtc atacatcgcg
     8581 cgggcgccaa tcccccggcg cgccaggaga ttgcttccct ggcacatagc ttgcgctatc
     8641 ttcttacatc ttcctcgcgc gtggtttcat agcgcaggct ggcgatagta ccgatcaaaa
     8701 cgtcaggttc tttgtactcg aaaacggact cgagcggaaa tgatctcgga ttagcattgc
     8761 aaatacgcgc ttaaaaatcg aagcgaccat aatagaaggc cggtctcatg taattcagtt
     8821 ctgatgtcgc tgcaggccaa cttatagcga ctcccgcact tgcatcccct ctgatcccta
     8881 cgttgaaaaa ttatcaacga aggcgcaggt tttttttgcc taacgctggg acctgcgcta
     8941 tactacgcgc cagtcacagt gcaaagcatc acgctttcaa tactcatctg gctcgtcctg
     9001 gtatcgcctg caagagctag ttacctgacc gctgcggagc agcttaaaga catcggtgcc
     9061 tcttttagct ggcaggacgt cagatcgcgc agtctaaaaa tcgtcgttga ataccaaatg
     9121 aacggcgaat cgagacgggc ccatctcggt tccggtttcc tgatatcggg cgatgggctc
     9181 ttcgtaacgg cgtatcatgt gatgtcctac tgcttgctca gccagaaggg caagagccgt
     9241 ttcgccgaaa gcgtcgattg ctcgattgcc aatccgcggg cgcgttacaa agctcaaaac
     9301 ggcgatcagg aatatgagat ccagatcttg tcgcatctga ccgaagagga ttcgaccaac
     9361 ggcaagaagc agcatacacc ggatgaaatc attaaaaatc gcgatttcgt tatcggcagg
     9421 ctcaaggccg acccgaagat ccatttttct cattgggagc tacgggatta cgagcagggg
     9481 aaaatcagtc tcagtaagcc tcgcgccgat ttcgagttga agccgttgat gccgcccagg
     9541 aaagtcttca ttgtcggtta tcccggcgat ggagagttcg aaattgccca cggattcttg
     9601 aatctgactg acgaaaaaca tcgcggctac tttgccgccg actataaact ctacgagcct
     9661 gcctatctaa aaaccgaggg aatcgagccc gacaccaagt ggggcatcgc ggttgccaat
     9721 cacatgagcg gcggcgtggt ggttgatgcc aaaggacatc cggtaggcgt cgtggttaac
     9781 ggcaaccaga acaccgccgg agttctttcc atcgaaaatg ttttggagac cttcttctcg
     9841 cggaaacgca gttccgagtc ccagccaaaa gtctttttgg cgcccaacag cacgcccctg
     9901 ttcctcaaac gcgcaccgga ttgagcggcg ccgcatcata gtacggccag ggtttcttcc
     9961 ttccatagaa gctgctcttt tgttgccatt gcgccgatct cttaaaggcc ggtggtgtct
    10021 cagtttgacg gttctctggg cggaccgtat attctaaaac tacagccatg cctattacgt
    10081 gggtagaaat gttactaaga atgggagcga gcgtttttct tggagctgcc attggatttg
    10141 agcgcgagcg cattcgtcgc gctgctggcc tgcgcactca ccttttagtc gctctggcat
    10201 ctgccacttt catgttggtt tcaacccagt tcgtctatta tcagcactac tccaaagatg
    10261 atctcgtcgg cgtggatagt tcccgtatag cagcaagcgt tgttaccggc gtgggattcc
    10321 tcggggcggg tgcgatactt agaaccggac tcagtgttca aggactaact accgcggcaa
    10381 gcctctggct agtttctgct gttgggctgg cttcgggcgc tgggatgttt gctgaagcag
    10441 gcaccgctac tatcgtaacg ctttttagtc ttgtcatact gcggcgattc gaaggtaaaa
    10501 ctaaagggaa cgggcagggc tagagcttca acgccgtact ggtcgatata aacgcagcca
    10561 tcggtgtgtt ctgtgaaaat tttgaaacca cctcttatat tgctctcata gcgaaaagga
    10621 ccgcgtgacc caaccagcta ggaaagctga tcgttgctat agctaatcag cacgagctat
    10681 gtagagcgta aatttgaccc tgcgaatgat gaaccgacgg ttcaccagac taacaaacgc
    10741 cttctccaaa aaatagaaaa tcacaaacat tctattgctc ttcattcatc cactacaatt
    10801 tctgccggat t
//
LOCUS       DASUVS010000111         8042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_473368, whole genome shotgun sequence.
ACCESSION   DASUVS010000111 DASUVS010000000
VERSION     DASUVS010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473368"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VF182_06290"
     CDS             <1..724
                     /locus_tag="VF182_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436720.1"
                     /translation="HTAEIMFLGMMPKVKPWDNKLVRQAVAYAIDRDTLIKTILRGEA
                     SRLDGPIGVGQYGYDPNLQPKYTYNPEKAKELLRQAGYPNGVDVELQTPVGRYTLDKQ
                     LSEAMVPMLNAVGIRTKLLTPEWATLWANVQNGKVPFYYMGRGGVVDPSAALSQYFET
                     GGSPRIGYSNPKVDELLSAERQAFDPAKRKKILGQVMSLITEEAPAHFLWRHQLLFGM
                     AKNIDYRPLPSERIYGWNMKVK"
     gene            972..1991
                     /locus_tag="VF182_06295"
     CDS             972..1991
                     /locus_tag="VF182_06295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6436721.1"
                     /translation="MAQGRVGYALGYGKFTNVREMAEVMQQAEERGFEMAFFSETIEL
                     MRDSVSSLAAIGLATKKLKLGTTQIVRLRGPVVMAQSLATLDELTGGRMSIAPGACTK
                     SHARVHALPEEVGATPTEVLRDYVESIRLLLKGEKCSYHGKYVNFDNVGLGWKPIRSN
                     IPIHLPATATVGLRLAGEIADGVILNAVCSPEYTVNALKIIKDSAEKAGRDFSRFQVA
                     QIINCSVEDDHRKALDAVRWEVATKLDPVQISFIAAPKMRVGEPYIRKEDIPLFEKAH
                     AEGGMDGLIKAIPDSYVEGMTASGTPHEVKTRVQQYRDAGVQIPLLRPAAAHQTQRLL
                     DLFAQ"
     gene            2084..4162
                     /locus_tag="VF182_06300"
     CDS             2084..4162
                     /locus_tag="VF182_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX6436722.1"
                     /translation="MYRAGIDIGGTFTDMLLVSEDGSAVIGKTLTTPADPSLAVENAL
                     KPALQKGIVNQQQRGTLIHGTTLVTNALIERKGAPTALLTTAGFRDAVEIGREHRYEL
                     YDLNLELPKPLVPRHLRFDVPERIAADGSVLQPLDEEFVRRLVAQLRDKEIKAIGVSY
                     LNSYRNPAHERRTGEIIGEVAPAIRVSLSSEVVSEIREFQRTSTTLANVYVQERVSDY
                     LAQLEARLEAIGFIGSFFVMLSSGGIATRDTASRFPVRLLESGPAAGALAAAQAGMLS
                     GHRDLLSFDMGGTTAKLCVIEDGRPLKTHEFEVDRVYRFRKGSGLPVRIPVIDMIEIG
                     AGGGSMARIDSLGLLKVGPESSGADPGPVCYGQGGTEPTVTDADLILGYLDARYFLGG
                     KMQLDVDAARNALGRFGRRLGISVEQVAWGIHQIVNENMANAARAHLGERGKDPRRLP
                     MYAFGGAGPVHGYGVAEILRLPALISPVGAGVGSTFGLLAAPLAFDFVRSAYNRLDEL
                     DWQFANSLLNEMAAEGRKVLESSGLLEEEISYQRTADMRYVGQGHEVSVSLPDGTLGV
                     VHLDTIARTFENTYEALYGRKGPDVPLEIINWRVVARGPQPQLEMKLQRESMTSGDAI
                     KGSRPAYFSALGGYGETPVYDRYALKSGMTFAGPAIVEERESTLIIGARGRARVDERL
                     QIVVDLIDGK"
     gene            4152..5882
                     /locus_tag="VF182_06305"
     CDS             4152..5882
                     /locus_tag="VF182_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020641032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX6436723.1"
                     /translation="MENKAIDPITLEVIWNRFLSVANEQQDALIRTAFSTIVRESQDL
                     ACGMFDTKGRMIAQSLSGTPGHINAMATSMQHFLAAFPADRLSPGDVLITNDPWQTAG
                     QINDITITTPVFKQGRLVALFANTCHSADIGGRILSAEAREVFEEGLRIPIMKLFDRG
                     QPNRILMQIVRTNVRQPDEVIGDFYAQTACNDAGGRALLEMMEEFELDSIDPVADEII
                     RRSETAVRAEIAKLPSGEWSNETWSDGFEEPIVVRCKVEVKSDEIFIDFTGSSPQSAR
                     GINVVLNYTHAYASFAIKAAICPDVPHNEGSFRPVHVSAPPGSILNALEPAPVASRQV
                     IGHFIPSAIFAALSGAMPGRLMAPGADPIWLSVWRGQNPPFTLTLFQVGGTGARPSKD
                     GLNAVGFPSGVAGVPAEIIESLSPVVMCRRQLRPDSGGTGKWRGGLGQLTEFTRRGDG
                     KWSVSSIADRTVYPAPGLLGGQAGATGEVFLGDGTRLHAKALVELKPGDIVHINLPGG
                     GGYGRPFERDPEKVRWDVIEGYITAEMAEREYGVAIRYNGDPDELVKLPDGWEIDRNK
                     TAELRQAKHA"
     gene            5967..6734
                     /locus_tag="VF182_06310"
     CDS             5967..6734
                     /locus_tag="VF182_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6436724.1"
                     /translation="MELHDRVALVTGGAGGIGGAVAHSLADAGLSGIVINYRSSEREA
                     DALAAEIESHGVKAVAIRADIQSDDEVRRMVSRIKDEFGRLDVLVNNAGVTHWVKLTD
                     LEGLTDAIWDEILDVNVKGSFRCARAAAEMLSERQGMIINVSSISGVLATSTISSLAY
                     GTAKAALIYMTKGLAVAMAPRVRVNCVAPAFTDTPWMSQHFGVDYQQVIANASAGYPL
                     QRIATPEDIAGAVLGLITGGDFVTGQTLMVDGGLSLS"
     gene            6747..7391
                     /locus_tag="VF182_06315"
     CDS             6747..7391
                     /locus_tag="VF182_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEX6436725.1"
                     /translation="MARAELIRELHDSRLVAVLRSKNAEEAMLTAKSAASAGVKFVEV
                     TLSVPGAFEVIEALARESTVHVGAGTVLSKKQAEAAITAGAQFVVSPSSELGLIPICH
                     AADVACYPGAATPTEIYAAARAGADLIKIFPADCLGGPNFIRQMAGPFPELRFMVSGG
                     VSLANIKEYVHAGVTGICLASAFLANTLAEQGERGLVNEVKEFVKFIARAKSQN"
     gene            7394..>8042
                     /locus_tag="VF182_06320"
     CDS             7394..>8042
                     /locus_tag="VF182_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436726.1"
                     /translation="MANIQLTLACEDYDRTRPIKDGRIKPEGIEINYLVMSVEEIFWR
                     MMKYEEFDVSELSMGAFLTGAARGRRPFVALPVFPSRTFRHRCIFINTASGIQRPEEL
                     RGKRMGVPEYSMTAAVWLRGLFQHEYGIPPTEIHWIQAGEEQPGRKDRVDFEMPPGVR
                     MDSKSDRTLNDMIASGEIDAMMSPRMPTCFLQGAPNVRRLFPNYRQAEMDYFAKTG"
BASE COUNT         1783 a         2083 c         2417 g         1759 t
ORIGIN      
        1 tcataccgca gaaatcatgt ttttggggat gatgccgaag gtcaaacctt gggataacaa
       61 gctggtgcgc caggcagtgg cttatgcaat cgatcgggac actctcatta agacgatcct
      121 ccgcggagaa gcgtctcgac tcgatggccc gatcggtgtc gggcaatacg gctacgatcc
      181 caatctgcaa cccaagtaca cctacaaccc ggaaaaagcc aaggagctgc taaggcaagc
      241 gggttatccc aatggagtcg atgtagaact gcaaactccc gtagggcgat acacgttgga
      301 caaacagctc agcgaagcga tggtgccgat gctcaatgcg gtaggcatca ggaccaagct
      361 tttgacgccg gaatgggcaa cgctttgggc caacgtgcaa aatggcaagg tgccgttcta
      421 ttacatgggt cgcggcgggg tggttgatcc tagcgcggct ttgtcccagt attttgaaac
      481 cggaggatcg ccccgtattg gctactcaaa cccgaaagtt gacgagctac tctcggccga
      541 gcggcaagcc ttcgatccag cgaagcgcaa gaaaatcctc ggccaagtga tgagcctcat
      601 tacggaagaa gcaccggcgc atttcctttg gcggcatcaa ttgctgttcg gcatggctaa
      661 aaatatcgac tatcggccat taccgagcga gcgtatatat ggatggaata tgaaagtgaa
      721 gtagaaggtg tcgggccggc cagacgatct gtataccgac cgttttcttc gggcaaccaa
      781 tccaacgcct cttagacaag ctgcgcattg ctctcaggag cgtaatccga tgcatcgccg
      841 taattcaggc ttgttcccgc cgagtaaatc ccctgcgttg acagtccagt cgcgttcgtc
      901 taggaatcag actataattt ttgttcaaat ccgatcaccg gaatacgtgt aatacttgga
      961 aggagttttt gatggcgcaa ggtagagtgg gttacgcact cgggtacggc aagtttacca
     1021 atgtccgtga gatggccgag gtcatgcagc aggccgagga gcgcggtttt gaaatggctt
     1081 ttttctccga gaccatcgag ttgatgcgcg attcggtgag ctcgcttgcc gcaatcggct
     1141 tggcgacaaa aaagctgaaa ttaggaacta ctcaaattgt tcgcttgcgc ggtccggtag
     1201 tcatggcgca atcgctggcg acattggacg aattgaccgg cgggcgcatg agcatcgctc
     1261 ccggagcctg cactaagagc catgcgcgcg ttcatgcttt gcctgaggaa gttggggcga
     1321 cgcctacgga ggtgctaagg gattatgtcg aatcgattcg cttgctgctc aaaggcgaaa
     1381 agtgctcgta ccatggcaag tatgtgaatt ttgataatgt agggttggga tggaagccga
     1441 tccgttcgaa tatcccaatc catcttcccg ccaccgcaac ggttggcttg cgcttggcgg
     1501 gagagattgc cgacggagtt atcctcaacg cggtatgctc accggagtac accgtcaacg
     1561 cgttgaaaat catcaaggac tcggcggaaa aggccgggcg cgacttttcc cgtttccaag
     1621 tcgcgcagat catcaactgc tcggtggaag atgatcatag aaaggctctc gatgcggtca
     1681 gatgggaagt tgcgaccaaa ctcgatcccg tgcagatttc gtttatcgcg gcgccgaaaa
     1741 tgcgcgtcgg cgagccctat atcagaaagg aagacatccc gcttttcgag aaggctcatg
     1801 ccgagggcgg catggacggg ctgatcaaag cgattccgga ttcctacgtt gaaggcatga
     1861 ctgccagcgg cacgccgcac gaagtgaaaa cgcgggtgca gcagtaccgt gatgcgggcg
     1921 tgcagatccc tctcctgaga ccggcggcgg cgcatcaaac ccaacgcttg ttggatttgt
     1981 ttgcgcagtg acaattcaac tcaggttcaa ctaacagtca ggaaagttgc tcctgagcag
     2041 tgggtgaacc agcgctcggg caaagcaact ctgtattaaa attatgtacc gggcgggcat
     2101 cgatattggc ggcacgttta ccgacatgct gttggtaagt gaagacggtt ctgccgttat
     2161 cggcaagacc ttaaccactc ccgcagatcc cagcctggcg gtggagaatg ctctaaaacc
     2221 ggcgctgcaa aagggtattg taaaccagca gcaacgcggc accttgattc atggcaccac
     2281 gttggtgacc aatgcgctca tcgagcgtaa aggcgccccc acagccttgc ttaccacggc
     2341 ggggtttcgc gatgcggtcg agatcggccg cgagcatcgt tacgagctat acgacttgaa
     2401 tttggaattg cctaaacccc tcgtgccgcg ccacttgcga ttcgatgtcc cggagcggat
     2461 cgccgcggat ggtagtgtgc tccaaccgct ggacgaagaa ttcgtgcgcc gtctcgtcgc
     2521 acagttgcgc gataaggaaa tcaaagccat cggcgtcagc tatctcaata gctatcgcaa
     2581 tcccgctcac gagcgccgta ccggcgaaat cattggagaa gtcgcgcccg cgatccgggt
     2641 ttcgctcagc tcagaagtgg tgtctgaaat ccgcgagttc cagcgtacca gcacgaccct
     2701 agccaatgtt tatgttcaag agcgagtgtc cgattatctg gcgcaattag aggctcggct
     2761 ggaggccatc ggattcatcg gaagcttctt cgttatgctc tccagcggtg ggattgcaac
     2821 ccgagatacc gcgtcccgtt tcccggtacg gttgcttgaa tccggcccgg cggcaggtgc
     2881 actggcagcg gcgcaggcgg gcatgctttc cggtcaccga gatttgttat cgtttgacat
     2941 gggcggcact actgcgaagc tttgtgtgat cgaggacggc cggccgctca agactcatga
     3001 attcgaggtc gatcgggttt atcgtttcag aaaaggcagc ggattgcctg tgcgcattcc
     3061 ggtaatcgat atgatcgaga ttggcgccgg cggcggcagt atggcgcgca tcgattcgct
     3121 cgggcttttg aaagtgggcc cggaaagttc cggcgccgat ccggggcccg tctgttacgg
     3181 gcaaggtggc accgaaccaa cggtcaccga tgcagacctg attcttggct acctcgatgc
     3241 gcgctatttt ctcggcggta agatgcagct agatgtcgat gccgcgcgca acgctcttgg
     3301 gcgatttggc cgccgacttg gcataagcgt ggaacaagtt gcctggggca ttcatcaaat
     3361 cgtcaacgag aacatggcga atgcggcgcg cgctcatctg ggagagcggg gcaaagatcc
     3421 gcggcggctg ccgatgtacg catttggcgg ggccggtccg gttcatggct atggtgttgc
     3481 cgaaattctg cgactcccgg cgctgatttc tccagtgggc gccggggtgg gatcgacgtt
     3541 tggcttactc gctgcgccgc ttgctttcga tttcgtccgg agcgcctaca accgattaga
     3601 cgagcttgac tggcaattcg ctaatagctt actcaatgaa atggcggcag aaggccgcaa
     3661 agtattggaa agctcaggcc tgttggagga agagatctct tatcaacgta ccgcagacat
     3721 gcgttatgtg ggacaggggc acgaagtctc ggtgtctctg ccggatggaa cgttgggagt
     3781 ggtgcatctg gacacaatcg ctcggacatt cgaaaatacc tacgaagcgc tatatggtcg
     3841 caaaggacct gacgtgccgc tcgaaatcat caactggcgc gtagttgccc ggggtccgca
     3901 accgcaattg gaaatgaagt tgcaacgtga atcaatgaca tccggcgatg ccatcaaagg
     3961 atcgcgtccg gcctactttt ctgcgctcgg tggttatggc gaaactccgg tgtatgaccg
     4021 ttacgccctg aagtcaggaa tgacgtttgc cggacccgcg atcgtcgaag agcgtgagtc
     4081 cactttgatc atcggtgcgc gcgggcgcgc tcgggtggac gagcgccttc agatcgtggt
     4141 ggacttaatc gatggaaaat aaagccattg atccgatcac gcttgaagtg atttggaatc
     4201 gttttctttc ggtggctaac gagcagcagg atgcgctgat tcgtactgcc tttagcacga
     4261 tcgtgcgtga aagccaggat ctggcctgcg gtatgttcga caccaaaggg cggatgattg
     4321 cgcagtctct gagcggcacg ccgggacata tcaatgcaat ggccacatcg atgcaacact
     4381 ttcttgcggc ttttccagcc gaccggctct cgccgggcga cgtgttgatt accaatgatc
     4441 catggcaaac cgcgggtcag atcaacgaca ttacgattac cacaccggtg ttcaagcagg
     4501 gccgcctggt cgcgttgttt gcgaatacct gtcacagcgc ggatatcggc gggcggatac
     4561 tttctgccga agcccgtgag gtgttcgaag aggggttacg gatcccgatc atgaaattgt
     4621 ttgatcgcgg tcagcctaat cggatactga tgcagattgt tcgtaccaac gtgcgccagc
     4681 ccgatgaagt catcggcgat ttctatgccc agaccgcttg taatgatgcc ggcgggcggg
     4741 cgcttctcga aatgatggaa gaattcgagc tcgacagcat cgatccggtg gcggacgaga
     4801 ttatccgccg ttccgaaact gcggtgcgcg cggaaatcgc taaattgccg agcggcgaat
     4861 ggagcaatga aacctggagc gatggtttcg aagagccgat tgtggtgcgc tgcaaggtcg
     4921 aggtgaagag cgacgaaatc ttcatcgact tcactggctc atcaccgcag agcgcccgag
     4981 gcatcaacgt cgtgctcaac tacacccacg cctatgccag tttcgccatc aaggcggcga
     5041 tttgtcccga cgtgccgcac aacgaaggta gtttccggcc agttcatgtc agcgctccgc
     5101 cgggcagtat tctgaatgcg ctggagcccg cgcccgttgc cagtcgccaa gtcatcggtc
     5161 actttatccc cagcgcgatt ttcgccgcac tctccggagc gatgcccggg cgcctgatgg
     5221 cgccgggagc cgatcccatc tggctgagcg tgtggcgagg tcagaatccg ccgtttacct
     5281 tgactctgtt tcaagtcggc ggcaccggcg cacggccttc aaaggatggc ttaaatgcag
     5341 tgggttttcc tagcggggtt gccggggttc ccgctgaaat cattgaaagt ctctcgccgg
     5401 tagttatgtg ccgccgccag cttcggcctg attccggtgg caccgggaag tggcgcggcg
     5461 gcctcggaca gttgacggag ttcactcgcc gcggtgacgg caaatggagt gtcagttcga
     5521 tcgccgatcg aacagtctat cctgcgcccg gacttctggg tggccaggct ggagcgacgg
     5581 gagaagtttt tttgggcgat ggaacgcgtc ttcatgcaaa agcgctcgtc gaactcaaac
     5641 caggcgacat cgtccacatc aacttgcctg ggggcggcgg ctatggtcgg ccgttcgaac
     5701 gcgatcccga gaaagttcgc tgggacgtga tcgaaggcta catcaccgcc gagatggcag
     5761 agcgagaata cggagtggca attcgttaca acggtgatcc cgatgaatta gtaaagttgc
     5821 ccgacggatg ggagattgat cgtaataaga cggcggaact acggcaagcc aagcacgcct
     5881 agcggcgcgc atcttaaatc caaaatcttg gatagcgacc cttgagatgt gagatccgca
     5941 gttgaaattc tgagcggagc gaagatatgg aacttcacga tcgtgtagct ctggttaccg
     6001 gcggcgccgg tggaatcggc ggcgcggtgg cgcatagctt ggccgatgcc ggtttgagcg
     6061 gaatcgtgat caactatcgc agctccgaga gagaggccga cgcattggcc gctgagattg
     6121 aaagtcatgg cgtgaaagcc gttgccatcc gagccgatat tcaatcggac gatgaggtcc
     6181 gccgtatggt cagcaggatt aaggatgaat tcggccggct agatgttctg gtcaacaatg
     6241 ccggcgtaac gcattgggtt aagttgacgg acctagaagg tttaaccgat gcgatctggg
     6301 acgaaattct ggacgtcaac gtgaaaggct catttcgttg tgcccgggcg gcggccgaaa
     6361 tgctctcgga gaggcagggg atgattatca atgtctcgtc gatctcggga gtgctggcta
     6421 cttcgacaat ctcttctttg gcatatggaa cggcgaaggc cgcgttgatc tatatgacca
     6481 aagggctggc cgtggcgatg gccccccgag tcagggtcaa ttgcgtggcg ccggcgttta
     6541 ccgatacgcc ttggatgagc cagcactttg gtgtcgatta tcaacaagtt atcgccaacg
     6601 catcggcagg atatccgctg caacgcatcg ctactcctga ggatattgcc ggagcggtgc
     6661 tcggtttgat taccggcggt gatttcgtga ccggccagac cttgatggtc gatggcggat
     6721 tgagcttgag ttagggaaca gaattaatgg caagagctga gttgattcgc gagttgcacg
     6781 atagtcgcct ggttgccgtt cttcgctcca aaaacgctga agaagcgatg cttacggcca
     6841 agtctgccgc aagcgccgga gtaaagttcg ttgaggtcac cttgagtgtt ccgggagctt
     6901 tcgaagtcat tgaggcgttg gccagagaaa gcacagtcca cgtcggtgcc ggaacggtct
     6961 tgtcgaagaa acaagctgaa gccgcgatta ctgccggagc gcaattcgtg gtatcgccgt
     7021 cgtcggaact cggcttaatt ccaatttgtc acgcggctga tgtcgcttgt tatcccgggg
     7081 cggcgacgcc gaccgagatt tatgccgccg cgcgggccgg cgccgattta ataaaaatat
     7141 ttcccgccga ttgtcttggc ggcccgaact ttatccgcca gatggccgga ccttttcccg
     7201 aacttcgctt catggtgtcg ggcggagtga gtcttgccaa tataaaggaa tacgttcatg
     7261 ccggagtgac cgggatttgt ctagcgagtg cttttctcgc caacacttta gccgagcagg
     7321 gcgaacgggg cctggtaaac gaggtgaagg agttcgtcaa gtttatagct cgagccaaat
     7381 cgcagaatta atcatggcaa acatacaatt gactttagcg tgcgaggact atgatcgaac
     7441 gcgcccaatc aaggatggcc ggatcaagcc tgaaggtatc gagatcaact acctcgtgat
     7501 gtcagtggaa gaaatattct ggcgtatgat gaagtacgag gagtttgatg tttcagagct
     7561 gtcaatggga gctttcctga ccggggcggc acgaggacga cgcccgttcg tagccctacc
     7621 ggtatttcca tcgcggacct tccgccatcg ctgcattttt atcaacaccg catccgggat
     7681 ccagcgaccg gaagaactac gcggcaaacg gatgggggtt cccgaataca gcatgacggc
     7741 tgcggtgtgg ctgcgcgggt tgtttcagca tgaatacggt attccgccca ctgaaattca
     7801 ttggattcaa gcgggtgaag aacagccggg ccgtaaggat cgggtagatt tcgaaatgcc
     7861 gcccggagtt cggatggata gtaaaagcga ccgaacattg aacgacatga tcgccagcgg
     7921 tgaaatcgat gcgatgatgt cgccacgcat gccgacctgt tttctccaag gcgcgccgaa
     7981 tgttcgccgg ctatttccca actatcgcca ggcggaaatg gactactttg ccaaaaccgg
     8041 ct
//
LOCUS       DASUVS010000112         5558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_36244, whole genome shotgun sequence.
ACCESSION   DASUVS010000112 DASUVS010000000
VERSION     DASUVS010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36244"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VF182_06325"
     CDS             <1..923
                     /locus_tag="VF182_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX6436727.1"
                     /translation="VLQVAGEPYSYQDASGELAGMAIGASLWGFANQVLENGDNIRTQ
                     RAIGTKFNYMTWEFESPIFPVVKAQGLSVKNWHDLILVNQVGKRFYDETKGDYPHGNV
                     CNDICPYTPNDYRNNENIEFHPTKYNFFNAAVAMNEYSQPPDYSAGPVWAIFDAEAVA
                     RNGWNVAPPYVDPDGYFFSANTLAELAAAIKNEYQAKPMKGATLEATVARYNSFVDAG
                     EDKDFGKPAPKFRIQTPPFYAAWGTPLVHDTRTGLRINAKCQVMDLNGQLIPGLYCAG
                     ESAGGFNQHGMGRCATQGFIAGRNAAVEKA"
     gene            complement(1116..1979)
                     /locus_tag="VF182_06330"
     CDS             complement(1116..1979)
                     /locus_tag="VF182_06330"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6436728.1"
                     /translation="MGIAYNPADNFEFKVSDVEFRGAPARTLMARIYQPQGDGPFPAL
                     LDLHGGAWNNQDRTANDRMDEKLAASGLLVVAIDLTLAPEAPYPASVQDANYGVRWLK
                     ANASKWNGDRATVGALGSSSGGHVIELCALRPRDPRYSAHSLTEASDIDATLAYVATR
                     SPVSDPFARFKQAEKMGREEMMQYSKIYFSPWETIHEGNPQEILDRREPVTLPPLLIM
                     QGELDDNVLPAVQEKFAASYRAAGGHCELEIFAGCEHRWVAEAGPQTDRAAEMVKDFI
                     ARQLGSFRRAA"
     gene            complement(2176..2370)
                     /locus_tag="VF182_06335"
     CDS             complement(2176..2370)
                     /locus_tag="VF182_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014154395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEX6436729.1"
                     /translation="MAERYKVDCRKFPSDQGCTVTISGSIDEVVELGWLHAKTHHAHK
                     DDEETGIKEWIRGNAETAND"
     gene            2573..3586
                     /locus_tag="VF182_06340"
     CDS             2573..3586
                     /locus_tag="VF182_06340"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436730.1"
                     /translation="MRIKSISVGWALAAVLVLSGSAPAQSSKLSQLKLAVSTATPHNT
                     PLWVAKDKKLFEKYGLDVQMLFVMGGSLVSQMLAAGEIQVAANAPAALVSLIAGGADI
                     AMFLGISNTSPFTLITQPAIKTAADLKGKRLGTARFGGSSHISALIALDHLGLDPKRD
                     RILLMQTGLDPERMAALEQKGLDAAMLQRLATKTMTSKGYTQLLNLTQAKIPYQNTVL
                     ASRRDHMAANPKLYDSFIRAVVEGYAYVFNKDNKQAVKEVLAKNLRLPSVDAAEDFYA
                     EALEELDRKPYPTVEGTQTVIKYVAEQNPKVASVKAERIVDSSWLKKLDEEGFFDKVY
                     KSK"
     gene            complement(3653..4447)
                     /locus_tag="VF182_06345"
     CDS             complement(3653..4447)
                     /locus_tag="VF182_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX6436731.1"
                     /translation="MNLARWALSWVAMFLLLAGSVGVSHTASDCAAPLEMIRFKTNLP
                     NTARAIRSGKNLVIVALGSSSTQGVGASNSTHTYPALLAEDLRRRWPQLPVTVINKGI
                     GGEMAFQMLARFERDVLPYHPQLVIWQTGSNQALRSMDIDGYTATIREGISRLKAAQI
                     DVVLMDPQFAPRVIARPMHLLVVDVIAALATEMKVPVFRRFALMRQWVSSGEYQMEDI
                     ISRDGLHMNDVSYGCVARLLADSLASAVGPETHARRQSHHARAASR"
     gene            complement(4444..5241)
                     /locus_tag="VF182_06350"
     CDS             complement(4444..5241)
                     /locus_tag="VF182_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX6436732.1"
                     /translation="MTPGFFRYVILISVITAGGIGFVPAPGRAFDQAHECPMPERFYE
                     FEPPLPKTAKALATGRNVVITVIGGSSTMGRAAGATALSWPARLGSVLSDRFPAAQIK
                     VVNRAVARQTAKQVLERLDRDILPVKPNLVIWETGTMEAVRGADVDAFRETVGTGIDE
                     LRAAGVEVVLMNMQFSRETDAMIYFEPYLTAMRQVADANDVPLFRRHGIMRYWAESGL
                     LDLRVREDEKRRELAVKLYDCIGRAMADFVTHGNGDAAAKAKSGTGR"
     gene            complement(5244..>5558)
                     /gene="opgC"
                     /locus_tag="VF182_06355"
     CDS             complement(5244..>5558)
                     /gene="opgC"
                     /locus_tag="VF182_06355"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HEX6436733.1"
                     /translation="NVLALALLVSRVIHPQAWFLVGRAARPFVICGRNSLHIFCLGIL
                     LSVLGQLVLNEYFGGFLMQLAVSAAGIAIMIGVAAFMEWFDAAQRMSRGIVAAPITGR
                     GA"
BASE COUNT         1203 a         1600 c         1601 g         1154 t
ORIGIN      
        1 acgtgctgca ggtagccggc gagccttatt cttatcagga cgccagcggc gaactcgcgg
       61 gcatggcgat tggcgcatcg ctttggggtt ttgccaatca agttctggaa aacggcgaca
      121 acatccgcac tcaacgcgcc atcggcacca aattcaacta catgacgtgg gagttcgaga
      181 gcccaatctt tccggttgtt aaggcccagg gattgagcgt gaagaactgg catgatctca
      241 tcctggtcaa tcaggtcggc aaaagatttt atgatgaaac caagggcgac tacccgcacg
      301 gcaatgtgtg caacgatatt tgcccttaca cgcccaacga ttatcgcaac aatgagaata
      361 tcgagtttca cccgacgaag tataatttct tcaacgctgc cgtggcgatg aacgaatatt
      421 cgcagccgcc ggattattcc gccggtccgg tgtgggcgat tttcgacgcc gaagccgtgg
      481 ctcgtaacgg ttggaatgtc gcgccgccgt acgtcgatcc tgacggatac ttttttagcg
      541 ccaatacgct ggcggaattg gccgccgcca taaagaacga gtatcaggcc aagccgatga
      601 aaggtgctac cctggaagcg acggtggccc gatacaactc gtttgtcgat gcgggcgagg
      661 ataaagattt cggcaaaccg gcgcccaagt tcaggattca aacgccgccg ttttacgccg
      721 cctggggaac cccgttggtg cacgacaccc gcaccggttt acgaatcaac gcaaaatgcc
      781 aggtgatgga cctcaacgga cagctgatcc cgggactcta ttgcgccggc gagtcagcag
      841 gcggtttcaa ccaacatggg atgggacgct gcgcgactca gggtttcatc gcaggcagga
      901 acgcggcggt ggagaaggcg tagagacggc tcccggccga tagcaaagac tccggcctcg
      961 tgagccgtac attcaaggtg gacggtgacg tgatgtctaa ccgttgccgg tcctaggcgc
     1021 gctgcccccg tactttgttc caagagcctg gagcgtcaat tttatatcgc caagcggcga
     1081 tcggggtcga agctcatcat ccgtccagcc ggaccctaag cggctcgccg aaaactgcct
     1141 aactgtcgcg cgataaaatc cttgaccatt tccgcggcgc gatcggtttg cggtccggct
     1201 tcggcaaccc acctgtgttc gcaaccggcg aagatttcca gttcacagtg gcctccggcg
     1261 gcccggtagg atgcggcgaa cttttcctga accgccggca aaacgttgtc gtccaattcg
     1321 ccctgcataa ttagcagcgg cggcaaagtg accggctcgc ggcgatcgag aatttcctgc
     1381 ggattgcctt cgtgaatcgt ttcccaagga ctgaaataga ttttgctgta ttgcatcatc
     1441 tcctcgcggc ccattttctc cgcctgtttg aaccgagcaa aggggtcact gaccggggat
     1501 cgcgtcgcca cataggcgag ggtcgcatcg atgtcgctgg cttccgtcaa cgaatgcgcg
     1561 ctgtagcgtg gatcacgcgg gcgcaaggcg caaagctcga tcacatggcc gccgctggaa
     1621 ctgccgagcg cgccgaccgt ggcccgatcg ccgttccatt tggatgcgtt cgctttcaac
     1681 catcgcaccc cgtagttggc gtcctgcacg gaagccggat aaggcgcttc cggcgccagc
     1741 gtcaggtcta tggcgacaac gagcagcccg ctggcggcaa gtttctcgtc cattctgtcg
     1801 ttggctgtgc ggtcctggtt gttccacgcg ccgccgtgca gatccagcaa cgccggaaag
     1861 ggcccatcgc cctgcggctg ataaattctt gccatcaagg ttcgcgccgg agcgccacga
     1921 aattccacgt ccgaaacctt gaactcaaag ttgtccgccg gattgtaagc aatccccatg
     1981 ctcttacctc ctgtgatgtt caatggaaaa agcccaagcc ttcatagtta agcgatcgcg
     2041 ccgttctagg tcccacattt agccgcatgg tgcgcgctcc aggtttaaac atgacgggtt
     2101 gtttatctgc gtatcattcc gggcctatta gactgcgcga tgcgctgtct acagctacgt
     2161 tcgcgtgaca ttcagctaat cgtttgctgt ttcagcgttt ccccggatcc attctttgat
     2221 gccggtttct tcgtcgtcct tgtgagcgtg atgtgttttg gcgtggagcc atcccaactc
     2281 gaccacctcg tcgatcgatc ctgagatagt aaccgtgcag ccctgatcgc tgggaaattt
     2341 tctgcagtcg actttgtatc gctcggccat gggatcaatc tcctttcccc acgagtgcgt
     2401 tttgatatgc ttctgccata gtaccggtcg cacggaattg caaccggaat atcaagcgaa
     2461 ccgatacaaa gcggctggat tggcacgcga ggaggaggtc gctactattc gggcgcgaaa
     2521 atatatgatt tgattagatc agcaggcgac acctaacttg gaggaaccgg caatgaggat
     2581 aaagtcgata tcggttggat gggcccttgc tgcagttctc gtgctcagcg gatctgcgcc
     2641 ggcgcaatct tcgaagctgt cgcagttaaa attggcagtt tctaccgcga ctccgcataa
     2701 cacgccgctg tgggtggcca aggataagaa actatttgag aagtatgggc tcgatgtcca
     2761 gatgttattt gtcatgggcg gatcgctcgt cagccagatg ttggccgcgg gtgaaatcca
     2821 ggtggcagca aatgcgccgg ccgctctggt gagtctcatt gctgggggcg ctgacattgc
     2881 catgtttctg ggcatcagca acacgtcgcc ttttacgctg atcactcagc ccgcgatcaa
     2941 gacagcggca gacctcaagg gaaagcgact gggcacggca cgcttcggcg gctcttccca
     3001 tatctcggct ttgattgctc tggatcatct gggcctggat ccaaagcggg acaggatcct
     3061 attgatgcaa accgggcttg acccggaacg gatggctgcg ctcgagcaaa agggcctcga
     3121 tgcggcgatg ctgcagcgcc ttgccaccaa aaccatgacc agcaaggggt atactcaact
     3181 cttgaacctc actcaggcga agattccgta ccagaatacc gtactggcct cacgcaggga
     3241 tcatatggcg gccaatccaa aactctatga cagcttcatt cgcgcggtag tcgaaggtta
     3301 cgcttatgtc tttaacaaag ataacaagca agcggtgaaa gaagttctgg cgaaaaatct
     3361 tagattgccc agcgtcgatg cggctgagga cttctacgcc gaggccctgg aagaactcga
     3421 tcgcaagcct tatccgacgg ttgaaggaac ccaaacggta attaaatatg ttgcggagca
     3481 aaatccaaaa gtggcctctg tcaaagccga gcgaatcgtc gattccagct ggctcaaaaa
     3541 actcgatgaa gaaggattct tcgataaggt atataagagc aaataatcgc gtgttgcggc
     3601 ggtgaatcgt agacactcgg attggccttc ctgaaataga acatggtcct ggttatctgc
     3661 tcgcagctcg cgcatggtgg ctctgccgcc gagcgtgggt ctcgggccct accgccgacg
     3721 ctagactatc ggccagcaga cgggcaacgc agccatagct cacatcgttc atgtgcaggc
     3781 catcgcgcga gatgatgtcc tccatctggt actcgccgct cgaaacccac tgccgcatca
     3841 gcgcgaaccg ccggaacacg gggaccttca tttcggttgc cagcgcggcg atgacatcca
     3901 cgaccagcag gtgcatgggc ctggcgatca cgcgtggggc gaactgtgga tccatcaata
     3961 cgacatcaat ctgtgctgcc ttgagacggc tgatcccttc gcggatggtg gccgtatatc
     4021 cgtcgatgtc catgctcctc agcgcctgat tgctgcctgt ctgccagatg acgagctgcg
     4081 ggtgataagg cagtacgtcg cgctcaaatc gcgccagcat ctggaacgcc atttcgccgc
     4141 cgatgccctt gttgatcacg gtgaccggga gctgcggcca gcggcggcgg agatcttcgg
     4201 cgaggagggc gggataggtg tgcgtcgaat tggaagcgcc gacaccctgg gtcgaggagg
     4261 aaccgagggc gactataacc aggttcttgc ccgagcggat cgcccgggcg gtgttgggca
     4321 ggttggtctt gaagcgtatc atctccagag gcgcagcgca gtctgaagcg gtgtgcgaca
     4381 cgccgacgga cccggccagc agcaagaaca tcgcaaccca ggaaagcgcc catcgagcca
     4441 ggttcatcgg ccggtcccgg acttggcctt ggccgcagca tccccgtttc cgtgagtcac
     4501 gaagtcggcc atagcgcggc cgatgcagtc ataaagcttc acggcaagct cgcgccgctt
     4561 ctcatcctct ctgaccctaa gatcgagcaa accgctctcg gcccaatagc gcataatgcc
     4621 atgacggcgg aatagcggga cgtcgttggc gtccgctact tggcgcatcg cggttaggta
     4681 cggttcgaaa taaatcatcg cgtcggtctc gcgcgagaac tgcatattca ttaacactac
     4741 ctcgactcca gcagcgcgca attcgtcgat gccggtccct accgtttccc ggaatgcgtc
     4801 gacatcggca ccgcgaactg cctccattgt gccggtctcc caaattacga ggttgggttt
     4861 tacgggcaga atgtcgcgat ccaggcgctc cagcacctgt tttgctgtct gtcgtgcgac
     4921 ggctcgattg acgaccttga tctgcgcagc tggaaaccta tcgctaagga ccgaacccag
     4981 acgcgcgggc caggatagag cggtcgcgcc ggccgcgcgt cccattgtgg aggatccccc
     5041 aatcacggta atgacaacat tccgaccggt cgccagagct ttggccgttt ttggcagcgg
     5101 cggctcgaat tcgtagaagc gctccggcat agggcactcg tgcgcctgat caaacgcacg
     5161 gcccggtgcg ggtacaaacc caatgccccc cgcggtgatg acgctgatca ggataacgta
     5221 gcggaaaaat ccaggtgtca tcgtcacgct cctctgccgg ttatcggcgc ggccacgatg
     5281 ccgcgtgaca tgcgctgagc ggcatcgaac cattccatga acgccgcaac gccgatcatg
     5341 atcgcgatac ccgccgcgga taccgcaagt tgcatgagaa aaccgccgaa atattcgttg
     5401 agcaccagtt gcccgagcac cgagagcaga atgccgaggc agaagatgtg aagggaattg
     5461 cgtccgcaga ttacgaaggg ccgcgcggcg cgaccaacca aaaaccacgc ctgcggatgg
     5521 atcacgcgtg agacgagaag cgccagggcg agtacatt
//
LOCUS       DASUVS010000113         3476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_473567, whole genome shotgun sequence.
ACCESSION   DASUVS010000113 DASUVS010000000
VERSION     DASUVS010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473567"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VF182_06360"
     CDS             complement(<1..547)
                     /locus_tag="VF182_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6436734.1"
                     /translation="MHRSRHQAAFILILLLGLAACGRVSTEQSVSDQPAHHVDGGFRN
                     TSADFQRPSSVTRWRFMARRLWASYTSPRTFEAPRMANNGKALRPGLIKPSITWVGHA
                     TLLVQMNGVNVLTDPQWSERASPVSWGGPRRLNAPGLAFEDLPPIHVVILSHDHYDHL
                     DFATVKRLADTHNPLFLVPLGM"
     gene            727..1098
                     /locus_tag="VF182_06365"
     CDS             727..1098
                     /locus_tag="VF182_06365"
                     /inference="COORDINATES: protein motif:HMM:NF037035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436735.1"
                     /translation="MNVDKLPAGHELDALTAERVFGWKDIARREHQPWGKRKDKGGRW
                     RAARVPDYSTDPTYAADIEERMHELGLSTQYRKELAKASRAKNLPTDWAPPDQRCRAA
                     IKAIKSKAKLPSITNKNREIL"
     gene            1183..2736
                     /locus_tag="VF182_06370"
     CDS             1183..2736
                     /locus_tag="VF182_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436736.1"
                     /translation="MAALNVPSATLAVFLSAAFVVLKPSGYEPRQAPRQSNVPLHVQR
                     GNKVQEKYDAYTERLHAYYESLVAALQVGTPELLPILEPPHPLQHGYQILPNIVPDPA
                     PLTKSARPQSAQYSWPWTDHLIESASSEVGQSVEELRRALSLEPKMQQVAYRQLATRY
                     RKMRAQQQNIDAHIQYNRLWQGAIAANRSKYDRENVLHDQVLKRQEILDVFRARNAAA
                     LTHAHGTIHQMVVASLNEISSGLTERGNLLAREIDAATDLSFIIPGYVRIEQQISNWI
                     IHVPFYTDIQDNEFVLSVKNAIEDTWHLRSSENEFRIALEFSYVSPEYLYGTHEPPRK
                     VTRINIQDHVDRFPPGRAILTTGALTTHARGRAIILGPHEITKRILAHEFGHILGFRD
                     DYFRGYKDLGEDGFQVREVVADANDLMGAPSTGAVLRRHYDRIIASHAINNCSDRGCL
                     EIRTSSMPMTTCNGRTLNSTDLKTPKPVEFIDTLASESRRTTAATRRSYNRNDDNRKH
                     AQADKQCYG"
     gene            complement(2745..2897)
                     /locus_tag="VF182_06375"
     CDS             complement(2745..2897)
                     /locus_tag="VF182_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436737.1"
                     /translation="MCEFKETKKIAAVLHSPAKVKEEHRVRRGLPARRALKDHPDLPD
                     QLVAGS"
BASE COUNT          848 a          895 c          950 g          783 t
ORIGIN      
        1 tcataccgag agggactaga aaaagcggat tgtgagtatc ggcaagccgt tttactgtgg
       61 cgaagtccag gtggtcgtag tgatcgtgcg ataggattac gacatgaatc ggcggcagat
      121 cctcgaaagc taggccgggc gcgttcaagc gccgcggtcc gccccatgaa accgggcttg
      181 cccgttcact ccattgcggg tcggtgagca cgttgacgcc attcatctgc actagcagag
      241 tcgcgtggcc gacccaggtt atgctcggtt tgatcaggcc gggtcggagc gctttgccgt
      301 tattggccat acggggagcc tcgaaagtcc gcggcgatgt gtagctcgcc cacaaacgac
      361 gcgccatgaa acgccagcgg gtcacgctcg acggacgttg gaaatcagca ctggtgttac
      421 gaaagccgcc gtccacatgg tgcgcaggct ggtcggagac tgactgttcc gtactcaccc
      481 ggccgcaagc agctagaccc aggagcaaga ttaaaataaa agcggcttga tgccggctac
      541 gatgcattta gcttttgtgt aacaaatcga ctgcgatgac aaccggtgat cgatggatgt
      601 gagtgggtta agaggcactt tccaagtgtg aacgcgagtc tatgtaaact ggctgatcct
      661 aagcggggca atggctgggc tttgccaggt tcgacgattt tgtgattgaa cagagacatg
      721 acgcaattga acgttgacaa actaccggca gggcacgagc tcgatgcttt gacggccgag
      781 cgggtctttg gttggaaaga tatcgcgcgc cgggaacacc agccttgggg caaaagaaaa
      841 gacaagggcg gccggtggcg cgcggcccga gttccggatt attcgaccga tcccacttat
      901 gcggctgata tcgaagagcg catgcacgag ctcggtttgt cgacgcagta tcgtaaagag
      961 cttgcaaaag cttcccgcgc caaaaacttg cccaccgatt gggcgccacc ggatcagcgt
     1021 tgccgcgccg cgattaaagc gatcaagtca aaagcaaagt tgccctcaat tacaaacaaa
     1081 aatagagaaa ttttgtgatc gcgcctcgct tcaggccaac caaacttcga gtgacgtatt
     1141 cttgctgtag acgaggttgc aaggtaaagt accggctatt ttatggcagc tctcaacgtg
     1201 ccttcggcga ctctggcggt ttttctgtca gctgcttttg tggtgctcaa accttctggt
     1261 tatgaaccgc ggcaggctcc tcggcaatcc aacgttccac tccacgtgca acgtggcaac
     1321 aaggttcagg agaaatacga tgcctatact gaacgtctcc acgcttatta tgaatcgctt
     1381 gtggctgcgt tacaggtcgg gactcctgag ctgcttccga tactggagcc acctcatccg
     1441 ttgcagcacg gctatcagat tttaccgaat atcgttcccg acccggcgcc attgacgaaa
     1501 tccgcgcgac cgcaatctgc gcagtatagt tggccttgga cggatcattt gatcgagagc
     1561 gcatcgagcg aggtaggtca gtccgtggaa gaattgaggc gtgctttatc gctcgagcca
     1621 aaaatgcagc aggtggctta tcggcagctc gcaacacggt atcggaaaat gcgcgcgcag
     1681 cagcaaaata ttgatgcgca tatccagtat aatcggctat ggcaaggagc gatcgctgct
     1741 aatcgatcga aatacgatag ggagaacgta cttcatgatc aggtgctcaa gcgtcaggag
     1801 atcctggacg ttttcagagc tagaaacgct gctgctctaa cacacgccca tggaacaatt
     1861 caccagatgg tggttgcgag tcttaacgaa ataagcagcg gtttaaccga gaggggaaat
     1921 ctgctcgcgc gcgagatcga tgcagctaca gatctttctt ttatcatccc gggctatgtt
     1981 cgcatcgagc agcaaattag taattggatc atccacgtac cgttttacac cgatattcaa
     2041 gataacgagt tcgttctgtc cgtgaagaac gctatagaag atacttggca cctgcgcagt
     2101 agcgagaacg agtttcgcat cgcgcttgaa ttctcctatg tttctcctga gtacctttac
     2161 ggaacccacg agccaccgcg gaaggttacc cgaataaata tccaggatca tgtcgataga
     2221 tttccaccag gcagggcgat tcttacgacc ggtgcattga cgacacatgc acgtggccgg
     2281 gcgattatat taggcccaca cgagattacg aaacgcattc tggcgcatga gtttggtcac
     2341 atccttgggt tcagagacga ttactttcgc ggctataaag atctcggtga agatggtttc
     2401 caggtcaggg aagtcgtcgc cgatgcgaac gacctgatgg gcgcgccgtc gacaggagcg
     2461 gttctgcgcc ggcactacga tagaatcatt gcaagccacg cgatcaataa ttgttcagat
     2521 cgtggatgcc ttgaaattag aactagcagc atgcccatga cgacttgcaa cggaagaaca
     2581 ctgaattcga cggatctcaa aacaccgaaa cccgtcgaat tcatagacac ccttgcgagc
     2641 gaatcacgcc gaacgactgc cgctacgaga cgcagctaca atcgcaatga cgacaatcgc
     2701 aaacacgccc aggccgacaa acaatgctac ggttgagtcc atccctagga acccgctact
     2761 agctgatccg gcaggtccgg atggtccttg agggcccggc gcgccggcag gcccctgcgc
     2821 acccggtgct cctctttgac cttcgctggg ctgtggagca cggctgcgat tttctttgtc
     2881 tccttgaatt cgcacatcga agttcgtatc gcctgcgcga ccttctactt tcgctccttc
     2941 atttttacgt tgggaatcct gggcagacac atagcttgct gcagacacag atactacgaa
     3001 cagagacaaa actattaggt atttcttcat cgctcacctc ctgttgatgg ttatatctcg
     3061 cttgttgcga cgaaccttat gaatcctggt agggagccaa actcatgcca atttgtatag
     3121 ctggcggcgg gcaccgatat tcgtgcattt cgcacggagc ttaaacgagg tgaacccgag
     3181 tcatttatgg aaacggaaaa gaggtagaaa gactaacgtt ccagatgtaa gactatgact
     3241 aggcgtagag ggagtggaaa tctcattaaa caatcaaaat caggcgcagc ctagtgtttc
     3301 atccgtgagt cgccccttct cacatgtgac acggcaggct gcgtggccgc ttcaacatag
     3361 cttttgtggc ggtctgtacg ggttagcgtc ttgctggcga gaatattgct cccgttgttt
     3421 gtggcggtag caccagagaa ggaggccgct gtgatcacaa ccagatttgg accgat
//
LOCUS       DASUVS010000114         4343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_256893, whole genome shotgun sequence.
ACCESSION   DASUVS010000114 DASUVS010000000
VERSION     DASUVS010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256893"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VF182_06380"
     CDS             <1..602
                     /locus_tag="VF182_06380"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX6436738.1"
                     /translation="WMARPGVVIIFAITLFGILYPIFKGRKERQVAAATGSAPVQTAR
                     RGGIHFGPAALFTLIIATIIAVALFESRNFGFRAGLFPWVIGIPTLLLALVQLAKDVT
                     RPKEARASEHEEISPEVARQRTVSIIGWTVGCFLAIWLLGFSYAVPLFVFVYLKFEGR
                     EGWLVSIAVTFFSWLFFHMLFERILNVPFPDPLLLSIFS"
     gene            complement(713..3004)
                     /locus_tag="VF182_06385"
     CDS             complement(713..3004)
                     /locus_tag="VF182_06385"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6436739.1"
                     /translation="MNDSRGYKVVGRAELRSDGAEKVAGKAVYTVDVDLPRMAHAKIL
                     RSPYPHARIGRVDSSKAERLAGVYAVITKEDQRELGMFGAAYKDQTIVAVDKVRYVGD
                     PVAAVAAIDEATAEEALALIDVEYQELPAVSSLEEALAPGAALVHDSASMGGELMGQR
                     YEAPKEFAGSNLCYRFSYAKGEIAEGFKKSDHVFENTFTFPRVQHFSMEPHATVAHVE
                     ADRITMWAATQEPFTLREHLADIFKVALNKIRIIVPYVGGGYGGKLAVKTEPLAAALS
                     AKAKRPVRLVHTVEESFKTVTRHPARVRIKTGVSKEGLLLARECLIHMETGAYADAGP
                     RVTQKAGYRSFGPYRIPHTKTDAYTVYTNTVPAGAYRGFGTLQVTWAYESQMDIIAEK
                     LGLDPLEFRLKNLLRKGELYTPGDTPVDCDLAEGLKRAAKEIGWGDNGAKTNRGKGLA
                     CCMKDGGGTYKVSSAAVKMNSDGSVVLLTGTVEVGQGARTALSQIVAEELSVPFDSVT
                     VAQLDTDVTPYDVNTNASSSTVVMGLSAQRAAQDLKGQLFRHAGKLLKAKPDQLTLRD
                     RRIYADAGASISFPELMQKVFLSRAGEIVGRGAYQDIKTKEAALGSPTTFWEISWGAA
                     EVEVDPDTGEVMLLKYVSLADVGQAIHPVLCEGQDEGGVMNAIGHTLLEEMIYKDGQL
                     LNPNLVDYRVPSFAHLPKDFETILVENHNGPGPFGAKGTGEGGLLPVAAAIGNAVRAA
                     TGVRFYDLPLTPERVWRALQPPK"
     gene            complement(3012..>4343)
                     /locus_tag="VF182_06390"
     CDS             complement(3012..>4343)
                     /locus_tag="VF182_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX6436740.1"
                     /translation="VGTGETRMISPDHQAVAIVKRLRDEGYESYLAGGCVRDFLLDKT
                     PQDYDIATSARPEDVQRIFPRTISVGAQFGVVLVVLDGQPFEVASFRFDGPYLDGRRP
                     SQVRYGSLQEDILRRDFTINGMVYDPIENRVIDLVEGKRDLARRRIRAIGDPRQRFDE
                     DRLRMVRAIRFAASLNFDIDSSTLDAIKQSAEGITRISSERIGEEITRILMEGGARRG
                     VELLDQTGLLQILLPEIEQMKGVEQSPEHHPEGDVFKHTLLTLSHLEAPTETLAYGCL
                     LHDVGKPVCFRKEADRITFYGHTDRGAEMAEATLKRLKRGRAAWERVAYLVKNHLRHT
                     QAPKMRLSTLKRFLREEGIDELLELARIDALSSSGDLQYYQFCQERLSEMQEEEIRPE
                     PLLRGKDLIEMGYTPGPIFAEILQQVEEAQLGGELTSRQAAIDWVKQKYHG"
BASE COUNT          876 a         1290 c         1134 g         1043 t
ORIGIN      
        1 attggatggc ccgcccgggc gttgtgatca tttttgccat cacgttattt gggatactct
       61 atccgatctt caagggtagg aaagaacggc aggttgccgc tgccactggt tctgctccgg
      121 tgcagacagc gcgccgcggc ggcatccatt ttggcccggc tgcgttattt actcttatca
      181 ttgccacgat aatcgccgtc gcgctttttg aaagccgcaa cttcggcttt cgcgccgggc
      241 tgtttccgtg ggtgattggc attccgactc tgctgttggc gttggtgcag ctggcaaaag
      301 atgtaacccg gccgaaagaa gcacgggcga gtgagcatga agagatttcg cctgaagtgg
      361 cccgacagag gaccgtctct ataatcggtt ggacggtggg ttgtttctta gctatttggc
      421 ttctgggttt ttcttatgcg gtgccgctct tcgtttttgt ttatttgaag ttcgaaggtc
      481 gcgaaggctg gctagtgagc attgccgtga catttttttc ttggctattc tttcacatgc
      541 ttttcgaaag aatacttaat gtcccgttcc ccgatcctct gcttctctcg attttctcat
      601 gatgcaagtt gccggaggtc ccgcaaggtg ctccgacaag ctttgccgga tgccctaagg
      661 gtatgtcacg cccaccggtt gcaacgagcc gatgaacggg gctcgaaagc aattacttcg
      721 gtggctggag cgcgcgccag actctctccg gtgtcagcgg taaatcgtag aatcgcactc
      781 cagtagcggc acggacggca ttgccaatcg cggcagcaac cggcagtaaa ccgccttcgc
      841 ccgttccttt tgctccgaag ggtccgggcc cgttatgatt ctcaaccaga atggtttcaa
      901 aatccttcgg caagtgagca aagctgggaa cccggtaatc gacaaggttc ggatttaaaa
      961 gttggccgtc cttgtagatc atttcttcta acaaggtgtg accgatcgcg ttcatcacgc
     1021 caccctcatc ctgtccctcg cacaagaccg ggtggatggc ctgtccgaca tctgcgagag
     1081 agacgtattt tagaagcatg acctcacccg tatccggatc gacttcaact tcagccgcac
     1141 cccagctgat ctcccaaaaa gttgtcggcg atcctagagc cgcctctttg gtcttgatgt
     1201 cttgataggc gccgcgtccc acgatctctc ccgcccgcga gagaaatacc ttctgcatca
     1261 gttcgggaaa agagatgctt gcgccggcgt cggcgtaaat tctccggtcc ctcagagtca
     1321 gctgatccgg tttggccttc aacagttttc ccgcgtgacg gaacaattgc ccttttaggt
     1381 cttgcgcagc tctttgcgcc gacaatccca ttacgacggt ggagctgctg gcgttggtgt
     1441 tgacgtcata gggggtcacg tcggtatcca gttgcgccac cgtcacggaa tcgaaaggaa
     1501 cgctcagttc ttcggcaaca atctggctca acgccgtgcg cgcgccctgg ccgacctcaa
     1561 ccgtcccggt gagcaaaacc acgcttccgt ccgagttcat cttcaccgcc gctgaagaga
     1621 ccttgtaggt tccgccgccg tctttcatac agcaggcaag acctttgccc cgattcgttt
     1681 tcgcgccgtt gtcgccccag ccgatttctt tcgcggcgcg tttcagaccc tctgccaagt
     1741 cgcaatcaac cggtgtgtcg cccggcgtat agagctcgcc ttttcgcaaa agattcttca
     1801 ggcgaaactc caacggatcc aatccaagtt tttcggcaat gatatccatt tgtgattcgt
     1861 acgcccatgt cacctggagt gtcccgaagc ctcgatacgc cccggcgggt acggtattag
     1921 tgtacaccgt gtaagcgtcg gtcttggtgt gcggtattcg ataaggaccg aatgagcgat
     1981 agccggcctt ctgagtcacc cgcgggccgg cgtcggcata ggcgccggtc tccatgtgga
     2041 tcaggcactc acgggccagt aaaagtcctt ccttgctgac tccggtctta atccgcaccc
     2101 gcgccggatg tcgggtcact gtcttgaaac tttcctcgac ggtgtgaacc aagcgcaccg
     2161 gacgtttggc cttggccgac aaggcagccg ccagtggttc tgttttcact gccagttttc
     2221 caccatatcc gccccccaca taaggtacga tgatacgaat cttgttaagc gccaccttga
     2281 agatatccgc caaatgctcg cgtagcgtaa acggctcctg agttgccgcc cacatcgtga
     2341 tccggtctgc ctcaacatga gcgacggtgg catgaggttc catcgagaaa tgctgcacgc
     2401 ggggaaaggt aaatgtgttt tcaaatacgt gatcagactt cttaaaccct tcggcgatct
     2461 cccccttggc atagctgaac cggtaacaga gattgctgcc ggcaaattct tttggagctt
     2521 catagcgctg tcccatcaac tcaccgccca tggaagccga atcgtgcacc aatgccgccc
     2581 ccggcgccaa tgcttcctcc aatgaagaca ccgctggcaa ttcctggtat tccacatcga
     2641 tcagggctaa cgcttcttcg gccgtggctt catcgattgc cgccaccgcg gcgaccgggt
     2701 cgccgacata gcgaaccttg tccacagcga cgatagtttg atccttataa gcggcgccga
     2761 acatgccaag ttcccgctga tcttctttgg tgatgaccgc ataaacgccg gccaggcgtt
     2821 cggccttact cgaatcaacg cgcccgatgc gcgcgtgagg gtaggggctg cgcaaaattt
     2881 tagcatgcgc catccggggc agatcgacat cgacggtgta aacagccttg ccggcaactt
     2941 tctctgcgcc gtccgatctg agctctgccc ggccgacaac tttgtagccc ctcgaatcgt
     3001 tcattgagac ctcaaccatg atacttttgt ttgacccaat caatcgctgc ctgacggctg
     3061 gtcaattcac cgccgagctg tgcctcttct acctgctgca agatttcggc aaaaatcggc
     3121 cccggagtgt atcccatttc gatcagatct ttgccgcgca atagcggctc aggacggatc
     3181 tcttcctctt gcatctcgga aagacgctct tggcagaact ggtagtattg caaatcaccg
     3241 ctggaggaca aggcatcgat acgcgccagt tctaaaagct catcgatgcc ttcttcacgc
     3301 aggaagcgct taagcgtgct cagccgcatc ttaggcgcct gcgtatgacg cagatgattt
     3361 ttcactaagt aagcgactcg ctcccaagct gctcgaccac gcttgagccg tttcaatgtc
     3421 gcttcagcca tctccgcgcc acgatcggta tggccgtaaa aagtgatacg atcagcctcc
     3481 ttgcgaaagc aaaccggctt tccgacatcg tgcaggaggc aaccataggc cagagtttct
     3541 gtcggcgctt cgaggtggct caaggtcaac agcgtgtgct tgaagacgtc gccttccggg
     3601 tggtgctcgg ggctctgctc aacgcctttc atctgttcga tctccggcag cagaatctgc
     3661 agcaaacctg tctggtccaa cagctcgact ccccttcgtg cgccgccttc catgagaatt
     3721 cgggtgatct cctcgccgat tcgttccgac gaaatccgtg tgatcccttc cgccgattgc
     3781 ttgatggcat ccaaggttga gctgtcgata tcaaagttca agctggcggc aaagcggatc
     3841 gcccgcacca ttctcagtcg gtcttcatca aaacgctgcc gcggatcgcc gatggcgcgg
     3901 atgcggcgtc tggcaagatc tctctttcct tcgaccaagt cgatcacccg attctcgatc
     3961 gggtcgtaga ccattccgtt gatggtgaaa tcacgtcgta ggatatcttc ttgcaggctt
     4021 ccgtagcgca cctggctggg cctgcgaccg tccaggtagg gaccgtcgaa tcgaaacgac
     4081 gccacctcaa aaggctggcc gtccaacacc actaaaacca ccccaaattg cgcacccacc
     4141 gaaatcgttc gagggaaaat ccgttgaacg tcctcgggcc gagcgctggt tgcgatatcg
     4201 taatcttgcg gggttttgtc gagcaaaaaa tcgcggacgc atccacccgc gagataactc
     4261 tcgtagcctt cgtcacggag ccgcttgacg atagcaacag cttggtgatc cggtgatatc
     4321 atccgggtct ctccagttcc aac
//
LOCUS       DASUVS010000115         7700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_475264, whole genome shotgun sequence.
ACCESSION   DASUVS010000115 DASUVS010000000
VERSION     DASUVS010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475264"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /gene="ligD"
                     /locus_tag="VF182_06395"
     CDS             complement(<1..917)
                     /gene="ligD"
                     /locus_tag="VF182_06395"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX6436741.1"
                     /translation="MRIPTDRDSVDVALEGKTVTLTNLRKIFWPDLGLTKGDLLQFYA
                     DVAPVLLPHLVNRAMVMKRYPHGIAGEFFFMKRAPSPRPEWIETCAIEHSSGNVIDFP
                     VIRDLASLLWVINLGCIDLNPWYARCDDVDRPDYLHFDLDPVKGASWEQLLETASVVH
                     DALESLKLPSLIKTTGSRGFHIYVPISRGPTQKEVWAVAKKFANTLAALRPDLMTAEY
                     RIAKRPNKRVLIDYNQNAWGQTLASIYSVRPQPKATVSTPITWKELKKGPRIEDFHIG
                     NVRARLNKLGDLWAPLLAQAGRARLEKLY"
     gene            1192..1932
                     /locus_tag="VF182_06400"
     CDS             1192..1932
                     /locus_tag="VF182_06400"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX6436742.1"
                     /translation="MEQPVKWRLGKSDIEKPEKTRGPLLKCLLLVAVLVGLSMAWRWT
                     PVSEWINFRTIVEWQNSVRAHPAALLFVVGTYLAGGLVLFPVTILNVATVFTFGPIRG
                     NAYGLAGWLASAALGYAIGRAFEHKLIHRLVGPRMDRLLEQAAGHGFLAVLTMRLLPV
                     APFTLVNIFVGTIPIRFRHFMLASVIGRIPGIIVLTFAGLQLEQALRNPGVGSFVAFI
                     VVLVLVLLAASLFVKRFKARHEQSRKSL"
     gene            complement(1993..2814)
                     /locus_tag="VF182_06405"
     CDS             complement(1993..2814)
                     /locus_tag="VF182_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6436743.1"
                     /translation="MKLFTTGEVAKILNLPGSRIRSFVRAGFLKPGRGKKKSLQFTFQ
                     DVLFLKTAKGLLDSRVPPKNIVRMLLSLKRQLPGDQHLSSLKIYADNRRVIAWDGKAR
                     WQPDSGQFLFNFDARAVVQKLNAPVTRAKTQASQLSAQHWFDLALELEESSVEEAQRA
                     YHMALEQDSKMAAAHLNLGKLYHDLGEMQKAEAHYRAAAESAPMDPAPRFNLGVLLDD
                     ARQIEAARRAYEEAIRLDPSFADAHYNLALLCESLNKKAEALAHFRTARKLYLNK"
     gene            complement(2811..3668)
                     /locus_tag="VF182_06410"
     CDS             complement(2811..3668)
                     /locus_tag="VF182_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX6436744.1"
                     /translation="MPPHSIGSGTISFGLVSIPVRLYSAASSQSVSFNLLHAKCGNRI
                     RQQRLCPVCNEVVERDDLVKGYEFAKDQYVKIEDEELKALEDEASNAIEISEFVPLSQ
                     VDPVYFEKSYYLGPDKGGEKPYRLLSDAMAQAGKVALAKFVMRGKENLVLVRSAQNGL
                     MLHAMYFADEVRNFDEIAKGESAKVTDAETNLALRLIEELSSEEFTPQKYEDEYRDRV
                     LELINSKAQGKEITIAAAPAPRAQVIDLVAALKESLGKKVPQEKKPAVRAKPAQPEKT
                     KKRAQSGKK"
     gene            3837..4526
                     /locus_tag="VF182_06415"
     CDS             3837..4526
                     /locus_tag="VF182_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436745.1"
                     /translation="MRRPLSVILNLLVCLSTSALPPLAWSLPLPSESPLRELVPLTSW
                     QRQFKITEGKDRGKVVPLTSEPDLANEKRWKLIFGNYAAVHVVREPGGALTMNRLDLP
                     NSRSFIIYDSPLPVMPSDIASSDLIHRETGYKMYSLATGKLKRAGRVTHQVKPASRSQ
                     FDTPAGRLEGYYVEIDHRMDMEYYSQLHLTVGLGCQLDHGPVYGSGQYTLTKLGVFTE
                     SKAAGAALVAQ"
     gene            4854..5399
                     /locus_tag="VF182_06420"
     CDS             4854..5399
                     /locus_tag="VF182_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436746.1"
                     /translation="MTAMTEVHKGRQALIAGDAEPALGHFRRATELDPDFLYFSSLPQ
                     TAITYTGRALYQLKRFSEARQAFEQARLRFPDDVMARPYLGITLVRQGERERGLQETA
                     AGLQHIHHWLDYIEANLPQGIYWDINREIRSEIERVLKQMTDDRLSAEQVIETAEWVG
                     NRMEWEIDAARRDEQASRNRE"
     gene            5701..6693
                     /locus_tag="VF182_06425"
     CDS             5701..6693
                     /locus_tag="VF182_06425"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436747.1"
                     /translation="MTVRLLFTAEAGAASLQSLPSSTQAETTLVQASPRASRPLRVAY
                     LSTSATMASLWMAKETGGFDKERVDVEVLSMASSVAIPALIANEIDAVQVSAAPVITA
                     SLRGHDVVFVAGLLNTMIWDFYARPEIKSPEQLKGKLIGTDRPATPVAYGTLVALKKM
                     GLTPKDVQLFPLGSSAQVVAALQSGQIIGGLGAPPASFVLERAGFRSMVNLLDIPYQN
                     VGIVVRRSRMDELGSRLVPLLRSVRAGIDRYYSDKPFAMKVIGKYTKEADADVLDKTY
                     EFYRRAGFRRELVVSEPGVQGILDFLSETIPEAKKAAPGQFFDDRFVRQVNSGK"
     gene            6782..7372
                     /locus_tag="VF182_06430"
     CDS             6782..7372
                     /locus_tag="VF182_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436748.1"
                     /translation="MTTAQRLPAFEKFIQELRAAWSELPDMQARMEKGASLLEELVND
                     STVKAAAKFWPSTEGRRNLLFYEDPDFNFAINGVVRTPGRKGSIHDHAHAWTAYGILD
                     GTEMLERFRRADDRSKEGYAKLELESVTEGQPGKVDLVPPFDIHAEQGGPARSVAIIL
                     RSERVAGKTTQGSYDLEKQTIRQIDGPIQVPFEMTG"
BASE COUNT         1688 a         2089 c         2079 g         1844 t
ORIGIN      
        1 tcatacaatt tttccaatct ggcgcgtccc gcttgcgcca gcaaaggcgc ccacaaatcc
       61 ccgagcttat taagccgtgc gcgaacattg ccgatgtgga agtcttcgat acgcgggccc
      121 ttctttagtt ctttccaggt aatcggagtg gaaacggttg ctttaggctg cggtcttacc
      181 gagtaaatag aggccaaggt ctgtccccag gcgttttgat tataatcgat cagaactcgc
      241 ttgttcggcc tcttggcgat gcggtattcg gcggtcatca aatctggacg cagagctgcc
      301 aaggtattgg caaatttttt agcgaccgcc caaacctcct tctgggttgg tccgcgactg
      361 atcggtacgt aaatatgaaa gcccctcgat ccggtagttt tgatcagcga gggtaacttg
      421 agactttcca gggcgtcgtg aaccaccgag gccgtctcca acaattgctc ccagcttgcg
      481 cctttcaccg ggtcaaggtc gaagtgtaaa taatccgggc gatcgacatc atcgcagcga
      541 gcgtaccagg gatttaaatc gatgcatcca agattgataa cccacaaaag tgaggcgaga
      601 tcccgaatca ccggaaaatc gatgacgttc ccggacgaat gctcaatagc gcaggtttca
      661 atccactcgg ggcgaggcga cggcgcgcgc ttcatgaaga agaattcgcc ggcgatgccg
      721 tgaggatagc gcttcatcac catcgcgcga ttcaccaggt gaggcaacaa aactggggcc
      781 acgtcggcgt agaattgaag cagatcgcct ttagttagtc ccagatcagg ccaaaatatt
      841 ttacgcaagt tggtcaacgt aacagttttc ccttccaagg cgacgtcgac gctgtcccgg
      901 tcggttggaa ttcgcaacgg acttgcggca gatgttctcg ctgtccgcag tttcttaccc
      961 tgccttgctg gaagaactct cgttgccgat ttggaaattg attgttggcg catcggtcta
     1021 gttaaaagca aagctcgcgc caccgatatc catcagccgg cgagcttcgg caactacgat
     1081 acgagttcaa tcattgaaac tgagttgaac ctacacacta tgggctgctt gtcccaatcg
     1141 cgagaatagg atcacttgct tcaaagggat tccccctcta ggttgataga aatggaacag
     1201 cccgttaaat ggcgtttggg aaagagcgac attgaaaaac cagagaaaac tcgcgggcca
     1261 ctgctaaaat gccttctcct agtcgctgtg ctagtcgggt tatccatggc gtggcgctgg
     1321 acaccggtca gtgaatggat caatttcaga actatcgtgg agtggcaaaa ctcggtgcgc
     1381 gctcatccgg cggcgctttt attcgtggtc ggcacctatt tagcgggagg tctggttctc
     1441 tttcctgtaa ccattctcaa tgttgccacc gtttttactt tcggcccgat tcggggcaat
     1501 gcctatggac tcgcaggatg gctcgccagt gctgcgctgg gctatgcgat aggccgagct
     1561 ttcgagcaca agctcattca ccgactcgtc ggcccccgaa tggaccggct tctcgagcag
     1621 gctgccggcc atggcttttt ggccgtgctt actatgcggc tcttaccggt tgcgcccttc
     1681 actctggtga atatttttgt cggcaccatt ccgattcgct tccgacactt catgctagcc
     1741 agtgtcatcg gcagaatacc cgggataatc gtcctaacgt ttgccggact tcagctcgag
     1801 caggctctgc gcaatccagg ggtcggcagc ttcgttgcct tcatagtagt tctcgtgttg
     1861 gtcctcctcg cagcgtcttt atttgttaaa cgcttcaaag cgcgccatga gcagtcgaga
     1921 aaatctttgt agtgaagatg ttttagaagt attgggctgg ggatcgtggc acgcggaata
     1981 ggacatcgct ccctatttgt ttagataaag ttttcgtgca gtacggaagt gcgccagagc
     2041 ttcggctttc ttatttaacg attcgcacaa tagagctaga ttatagtgcg cgtcggcgaa
     2101 gctgggatcg agtctgattg cttcttcata ggcgcggcgg gcggcttcga tttgccgggc
     2161 atcgtcaagc aacacaccaa gattgaatct cggcgccgga tccatcggtg cgctttcggc
     2221 agcggcgcgg tagtgggctt ccgccttctg catctcaccg agatcatgat aaagctttcc
     2281 cagatttaga tgtgccgccg ccatctttga gtcttgctcc agcgccatgt ggtaagcccg
     2341 ttgagcttcc tcgactgaac tctcctccag ctccagggct agatcgaacc aatgttgagc
     2401 gctgagttga ctggcttgcg tctttgcccg agttactggc gcgttcagtt tttgcaccac
     2461 ggcccgggca tcgaagttaa acaaaaactg tcccgaatcg ggctgccagc gtgccttgcc
     2521 gtcccacgcg atcactcgcc gattgtcggc atagattttg aggctggaca gatgctggtc
     2581 tccgggaagc tgccgcttca gagacaacag catgcggacg atatttttgg gtggaacacg
     2641 ggagtcgagg agacctttgg ctgtctttaa aaagagcacg tcctgaaagg taaactgcag
     2701 cgatttcttc ttgccgcggc cgggcttgag aaatccggcg cggacaaagg aacgaatgcg
     2761 actgcctgga agattgagga tcttcgccac ctcgccggtg gtgaacagtt tcatttctta
     2821 ccggactgag ccctcttttt agttttctca ggctgggctg gcttggcccg aactgccggc
     2881 ttcttttcct gcggaacttt tttgccgaga ctttccttga gcgcggccac caggtcgatg
     2941 acttgcgcgc gcggcgccgg cgccgcggcg attgtgattt ctttgccctg cgctttgctg
     3001 ttgatcagct ccagaacacg atcgcggtat tcgtcttcat atttctgcgg cgtgaactcc
     3061 tcgctcgaga gctcctcgat cagcctgagc gccagattgg tttccgcatc cgtgaccttc
     3121 gccgattcgc ctttggcgat ctcgtcgaaa ttccgcactt cgtcggcgaa atacatggca
     3181 tgaagcatca agccattttg cgccgaacga accaagacta aattttcctt gccgcgcatg
     3241 acgaacttgg ccaaggccac cttgccggct tgcgccatcg catcggacaa caaacgataa
     3301 ggtttttcgc cccctttgtc gggaccgaga tagtaactct tctcgaagta aacaggatcg
     3361 acttgcgaca gcggcacgaa ttcggaaatt tcgatggcat tggacgcttc atcctcgagc
     3421 gctttcaatt cctcgtcctc gatcttgacg tattgatcct tggcaaactc atagcctttg
     3481 accaaatcat cacgctcgac cacttcattg cagaccggac aaaggcgctg ttgccggatc
     3541 cggttgccgc atttggcgtg aagcagattg aagctcaccg attgcgacga ggcggccgag
     3601 tacagcctaa cgggaatcga tactaaaccg aaggaaatcg taccggatcc aatagaatgc
     3661 gggggcatga tggttcctcc ttgaagtcta agcaagctcg ggcataatca acagggcctg
     3721 ttttgtatta taccaggcgc gggaaatttg aaatagcagc cgcattatga tctagtatgt
     3781 caagcctaac cccgctcatg cgcgctgaaa acgcgcaagg aggagagaaa agcccgatgc
     3841 gtcgtccgct ttctgttata ctcaatttgc tggtctgctt atcaacttcg gcattaccgc
     3901 ccctagcctg gtctttgccg ctgccttccg aatctcctct gcgtgagctt gttccgctaa
     3961 ctagctggca acgacagttt aagatcaccg agggcaaaga ccgcggcaaa gtggtcccgc
     4021 tgacgtccga acccgatctc gcaaacgaaa aacgctggaa acttattttc ggcaactacg
     4081 ctgccgtgca tgtggttcga gaacctggcg gcgcattgac tatgaatcga ctcgacttgc
     4141 caaatagccg tagttttatc atctatgatt ctcccctgcc ggtgatgccc agtgatattg
     4201 catcctcgga ccttattcat cgggagaccg gttacaagat gtacagtctc gctacgggca
     4261 aactcaagcg ggccgggcgc gtgacccatc aggtaaaacc cgcatcgcgt tcgcagtttg
     4321 acactcccgc tggtcgtctc gagggatatt atgttgagat cgatcatcgc atggatatgg
     4381 aatattacag tcagctgcat ctgacggtcg gcctcgggtg ccagttggac catggcccgg
     4441 tttatgggtc ggggcagtat acattaacta agctgggtgt atttactgaa agcaaagcag
     4501 ccggagccgc gctggttgcg caatgatcga cgatagcttt ttccgatcat acgcagagta
     4561 ctccagagtt ccctcctata cccggacgat ggcggtttcc gatcacagtt attaaccctt
     4621 gctaatctcc tgtgacaacg agacgtgaag ccggttaagg tttagagccg cgcgatttct
     4681 ccctctcgac tatttcgccg ctggtccgcc gctcaacaat cgaacgccct agcgcgcttc
     4741 ttgcacggtc aggcagcaca tattattgaa gaattgccaa cgtggttacg aggggtgcct
     4801 ttttcgtact aatcattctt tttgtcctca gccatgctgc ttgcaagaga tcaatgactg
     4861 cgatgactga agtgcataaa ggcagacaag cgttgattgc cggcgacgcc gagcccgccc
     4921 tcggtcattt ccgccgcgct actgagctcg accctgattt cctgtatttc agctcgctcc
     4981 cacaaaccgc gattacctat accggtcgag ctctttacca gctgaaaagg ttttcggaag
     5041 cgcgtcaagc cttcgagcag gccagattac gatttcccga tgatgtgatg gcgcgaccgt
     5101 atctaggcat caccttggtg cgccaaggcg aacgagagcg cggtttgcaa gagactgccg
     5161 cgggactgca acacatccat cattggctcg attacatcga agccaatttg ccccagggaa
     5221 tttactggga tatcaatcga gagattagat ctgagatcga gcgggttttg aagcagatga
     5281 ccgatgatcg attgagcgcc gagcaagtaa tcgaaaccgc tgaatgggtc ggtaatcgga
     5341 tggaatggga gatcgacgcg gccagacggg acgagcaagc gagccggaac cgggagtgat
     5401 gcgcttcgat ttcaagccgc tcgatcttga atccgtgcac gcgctcgcgc gaaaggccgg
     5461 ctctgtagcc agatgatgtg aatgataagc caggtgcggc actgttgcct gcttattctc
     5521 gagatgccta ctttgatccc aactgacttg cggctttgac tcgactgtga gcagaccatg
     5581 aatccatcga gaaaagaagc atgccctgga atatgtcgaa cagccgcccg gctgttttgc
     5641 aggtcgctgg cttgtgtttt gcgccaccgg ctcactgccg atgctttgct gatgattgga
     5701 atgaccgttc gactgttatt cactgctgaa gcgggcgcag cctcattgca atcgctgcca
     5761 tcttcaactc aggcggaaac gactctcgtg caagcatctc ctcgagctag ccgacccctg
     5821 cgggtcgcct atctcagcac gtcggcgaca atggcgtcgc tgtggatggc taaagagacc
     5881 ggcggcttcg ataaagagcg cgttgacgtc gaggttttgt cgatggcttc gagtgtcgcc
     5941 ataccggcgc tcatcgccaa tgaaatcgat gccgttcaag tctcggctgc gccggtaatt
     6001 accgcttcct tgcgcggtca cgacgtcgtt tttgttgccg gactgctgaa cacgatgatt
     6061 tgggacttct atgcgcggcc ggaaataaaa agcccggaac aactcaaggg caagctaatc
     6121 ggcaccgatc ggccggccac tcccgtggcg tacggtaccc tggtagctct caaaaaaatg
     6181 gggctgacac cgaaagacgt tcaactgttt cctctcggca gcagcgccca agtcgtcgcc
     6241 gctctccaga gcgggcaaat tatcggcggc ttgggggcgc ctcctgccag ctttgtcctc
     6301 gagcgcgccg gctttcgctc catggttaat ttgctggata ttccgtatca gaatgtcggc
     6361 atcgttgtgc ggcgcagccg catggacgag cttggctcgc gcttggtgcc gctcttgcga
     6421 tcggtgcgcg caggcatcga tcgctattac agcgacaagc ccttcgcgat gaaagtcatc
     6481 ggcaaataca ccaaagaagc cgatgccgac gtgctcgata aaacctatga attttatcgc
     6541 cgcgcaggct tcaggcgcga gctggtagtt tccgagcccg gtgttcaggg tattttggac
     6601 ttcctgtcgg aaaccatccc ggaagcaaag aaagcggcgc cggggcaatt tttcgatgat
     6661 cggttcgtgc gtcaagttaa cagcggcaaa tgatttttcg gtgtcacatt cgctacgaga
     6721 ttggcaagta gataataaaa tactcgccgg gatcatagtt gccggcggtg ttggagatca
     6781 catgaccact gcacaaagac ttccggcatt cgagaaattt attcaggaac tgcgcgctgc
     6841 ctggtccgaa ttgccggaca tgcaagctcg catggaaaag ggcgccagcc tgctggaaga
     6901 gctcgtcaac gattctaccg ttaaggcggc cgcgaagttc tggccttcaa ctgaaggacg
     6961 cagaaatcta ctcttctacg aagatccgga cttcaatttt gcgatcaacg gcgtcgttcg
     7021 caccccaggt cgcaaaggga gtatccacga tcacgctcat gcctggacgg cttacggtat
     7081 tttagacgga actgaaatgc tcgaacgatt ccggcgcgcc gatgatcgga gcaaagaagg
     7141 ttatgccaag ttggagttgg aaagcgtgac cgaagggcaa ccaggcaaag tcgatctggt
     7201 tccgccgttc gacatccatg ccgaacaggg cggccccgcc agatcagtgg ctataatcct
     7261 gcgctcggaa cgggtcgccg gaaagaccac gcagggaagt tatgatttgg agaagcaaac
     7321 cattcggcag atcgacggcc cgatccaagt gccattcgaa atgaccggct gagcgccagc
     7381 gctccgcatt catggcaagc cagagtccta ctcttctttg caccctttag ctagtagctt
     7441 tgaacccgga ttccgctgtc agaaaataag cgaggggcaa ccctggagaa gcgagacacc
     7501 ggatgtgcaa tttcaaagtc ttttttgacg cttggccggc cgcctctatc tgcttgacac
     7561 cgttgagcac ggcactggca gctgtcgcct gctcagaggt agaactcatc gttcgctgcg
     7621 gaaggtttgc tcctcgctca gtgactcgcc ttcaactgcg gaatccgggt ttgaatcctt
     7681 ctcttattcc atactcagtc
//
LOCUS       DASUVS010000116        23098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1130915, whole genome shotgun sequence.
ACCESSION   DASUVS010000116 DASUVS010000000
VERSION     DASUVS010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 23098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23098
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130915"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VF182_06435"
     CDS             <1..770
                     /locus_tag="VF182_06435"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX6436749.1"
                     /translation="VPASAPVYQPKFYPYETGEKASYRASWNGVPVATAEIETIPITF
                     EGKKSYRVRVDAKTSRGLDLIWKMRDTITSVFDAKSLEPSRFVFSQRENSRVIDTQAK
                     FNQAAKKWAVNRWQEGKKPRVYDFDSNNTLDPITAVYLARSIDLKVGDKLFFNVFGGR
                     YQYLLELNVEKKEPVELESGAVIQAFKLVPKLTNLTKKGYAGRLQEAAIWVSADERRI
                     PVKFWSRIFAGNVYMELVQDQTGVQSANSVDSPAPAS"
     gene            877..1506
                     /locus_tag="VF182_06440"
     CDS             877..1506
                     /locus_tag="VF182_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX6436750.1"
                     /translation="MLSGFRYPSAPRHRAGSATAYSDSNRRGDAQIEDRLRRTILPLL
                     GAMDRPCRPEELRVQIMNEPEINAANAGGCQFFVTSGLLQRANDDHLLGVMAHEIAHQ
                     DLGHAVKSQMLGAGLSIGAALLQQILPASGAIAPLAGTLIARGHSRTEEYAADRHALV
                     LLRRIGYSPEIMIDTLSWIQRASGDQQPGSFLSTHPALNDRISALKKTL"
     gene            1750..2118
                     /locus_tag="VF182_06445"
     CDS             1750..2118
                     /locus_tag="VF182_06445"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEX6436751.1"
                     /translation="MNINLKAMKKLNGIAAVAILSGTLLAGCAAGGLTTREKGAGIGA
                     LGGAAAGGLIGSAVRHPGAGAAIGAALGLGAGAVIGDQLQGRENQSYEHDQQIYRNQQ
                     EINRQREQLEEIRRRDNEYY"
     gene            2099..2281
                     /locus_tag="VF182_06450"
     CDS             2099..2281
                     /locus_tag="VF182_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436752.1"
                     /translation="MTTSTTRLEHQWGGTGLSMWNTFSHQWDYLVLNLIVGVYMMLGA
                     ALGLTLMWLFLTAPTP"
     gene            2383..3234
                     /gene="rfbD"
                     /locus_tag="VF182_06455"
     CDS             2383..3234
                     /gene="rfbD"
                     /locus_tag="VF182_06455"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX6436753.1"
                     /translation="MKILVTGAAGLIGSHFARRFGAARDVLALTRKDLDITDQAAVRS
                     CVAAERPSLLVNCAVIQVDEAEQNHAKAAAVNVEGARFLAAAAAQYSAEIIQFSTQYA
                     FDGEIIGRAPYTIRDQPRPINFYGRTKVAAEEVVRAACAQSYIVRTSWVYGEGKNSFL
                     CTVHNDLGAGKRVRAIDDVWSSTTYVDDLTDRCLEILKLRRYGTYHIVNEGVCSYYDF
                     ALEAGRLKGLGKERLGSLIEVVQERDLQRVALRPRYTPMRCLLSEELGLAPMRHWRDA
                     LAHYVKN"
     gene            3514..4488
                     /locus_tag="VF182_06460"
     CDS             3514..4488
                     /locus_tag="VF182_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436754.1"
                     /translation="MKPFLLLTSVFFLVAFSRSAPAQKLTLAHVAIHPGQGMFWVAKD
                     SGLLAKHGFSAEIVLIPGSPRTVQALIAGDLDYAVAGAPAYVRARTQGADVVILSSLS
                     GYSSQRVLLKPDSTVQTFNDIRGKTIGVTQYGSAGDSFIRSALKKAGIKETEVTILQM
                     GGTPGVSQALEAGRIEVGVLGESGALLVFRGKARFLKGGSSKEFGFHALDAPLGTTER
                     KIKADRGSVLRFMQAYVEAIHYFKSNRAGTIKILQKYMAGLSEEHVALWYDDYREGLV
                     SLPYPDDNGLRAELDLINVPRSQSLAAYVNKSFLDEIKNSGLVERLNK"
     gene            4503..4868
                     /locus_tag="VF182_06465"
     CDS             4503..4868
                     /locus_tag="VF182_06465"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6436755.1"
                     /translation="MAKIRHLAIKAEDQERTATFYKETFGMSEAWRGPVREDGHRAIY
                     LTDGYINMAILPARGGREGIDHFGFQVEDLEGTLKTAASTGAKSDSYKKPRDGRFAEM
                     GVQDPVGTPVDVSVHGWKA"
     gene            4937..5938
                     /locus_tag="VF182_06470"
     CDS             4937..5938
                     /locus_tag="VF182_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436756.1"
                     /translation="MTKTIWLTVVLLHVFVMPACPSLAAEPSKLLIAHGAISNNVAPL
                     WIAKEQGIFRKYGIEADLIFIIAGRAMQAMLAGQVPVGLVGGTHVTNANTGGGDFAMI
                     LGLENRLDYIFVAKPTIKGAEDLKGKKVAIGTPAGSASLATYVSLDHLGLVPRRDQIT
                     LLGIGGVPERMGALRSGSVEATSLSPEIGQVVVSEGYRVLVDLGKEDVPFQSSGLVVP
                     RSLIKTNPQLVENIAKATVEGAAFVLKPANKEIVLRSLGRNLRLDKPDRVEKAYQGLL
                     ANLPRPPCPSLEGISAMLKLMAQHGLNAKASQIKPEEIADMSYCKKFEESGFFRSLY"
     gene            complement(6191..7417)
                     /locus_tag="VF182_06475"
     CDS             complement(6191..7417)
                     /locus_tag="VF182_06475"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6436757.1"
                     /translation="MPSPASNIRPWSSLLIRDYRLVWSASVFNAVAVQVRNVAGLYQV
                     YEISGSPLQLGLTGFFQAFPFVIFGLFAGAVADTFDRKKLLIATVSIQFIPSLLLGIL
                     TVTGSIQAWHVYTLGFLGALVEVFNWPARSALIPRLVPRPLLMNAVTLNTIIIQMSFL
                     LGPAIGGILIDRVGLSTTYFITNAMLLPAAIAIVAMRTSGVPEGVRRTINLRSIFEGI
                     EFIWVQRIILSLFLLDFGVTLVGFYRPILPIFAADVFHTGASGLGLLYGAPSLGAIVG
                     SLALLMLGDIARKGVLVVAAALIFAGSLAFLGVAQWFWMAVGATLILGFTDSVSVAIR
                     RTVVQLQAPDGMLGRASSLISVFAQATNGLGAILAGVAAHYVGAPNALLLGSLLCFIM
                     IAAICIAIPQLWRYRS"
     gene            complement(7712..8005)
                     /locus_tag="VF182_06480"
     CDS             complement(7712..8005)
                     /locus_tag="VF182_06480"
                     /inference="COORDINATES: protein motif:HMM:NF039404.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6295 family protein"
                     /protein_id="HEX6436758.1"
                     /translation="MCTMIVHQAKIEGRGKNGGDWFELSEANISYDHPYDMPLEHALN
                     IDFVNEAQGPAARVAVELTIEAARNLVKSIETVLAQAEERGVLEDIRLAASTR"
     gene            complement(8106..8861)
                     /locus_tag="VF182_06485"
     CDS             complement(8106..8861)
                     /locus_tag="VF182_06485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6436759.1"
                     /translation="MYRTDQYEGMIAETVSMQGHKGEAINAYVARPLGAGPFPGMVLI
                     HHAPGWDEWYRECTRKFAHHGYVSISPNLYFREGHGSPEDVGAKVRAAGGVPDEQVLG
                     DLDGAKNLLRSLPYVSKVGVFGTCSGGRHGFLAACKLKGFDALVECWGGGVVMKQEEL
                     TPKRPVAPIDFTKDLSCKMIGIFGNDDKSPSPEQVNQHEAELKKHGKSYEFHRYDGAG
                     HGFFYYDRPAYRQEQAVDGWKKVLGFLEKNLKG"
     gene            9182..9523
                     /locus_tag="VF182_06490"
     CDS             9182..9523
                     /locus_tag="VF182_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX6436760.1"
                     /translation="MSSKIYVGGLPYSTTDDQLQEIFSAHGAVESARVITDKFSGRSR
                     GFGFVEMSSSDEAQKAIQALNGSDLGGRTLTVNEARPQERRSGGGFGGGGGGGGGGGE
                     RRGGGGGRNRW"
     gene            complement(10008..11555)
                     /locus_tag="VF182_06495"
     CDS             complement(10008..11555)
                     /locus_tag="VF182_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436761.1"
                     /translation="MSTKSRCWSRREFIKKSAAASAVLSFSHFHAISPIAEAQSLNKN
                     RVTVAHGVGVYSLNPYAVTTSPIQGVWGSVMETLIDFDYDKREYRGILAESWTVKGNK
                     LHFAIRKGVRFHDGTPFTGKDVMASLKRMITDKQSLVAPSLQNIKEMELPDDFSFVLT
                     LKKADATALEDLNNRVIMKQAVAEKMGEADNPPIGTGPFKFVSWERSGNFVMRRNENY
                     WGPPAKIEEILYKVIKEDAARIAALESGQADLISNIPPHEVARLKSNPRLQVQPVQGL
                     RPIFLVLSPAYKPLDNPKVRRAITQAIDREGLIKHILEGNAYPLLGLLSPQVFGYDPG
                     AKAYPYDVQNSKQLLKEAGFGNGFEIEYYSPTGRYPKDLEIAQVIVEQLSKVGIKAIL
                     KTPEWSIFNTDYKNGKYPMYLTGRGSLTDANALFHQYFRSGVTKRVIGYSNPKLDEIL
                     DTEQQTFDVKKRERLLGDAHRIILEDAAAIPLWNSMDIYAHRGDLVWTPPPDEKVQLK
                     HASYKSK"
     gene            11781..13109
                     /locus_tag="VF182_06500"
     CDS             11781..13109
                     /locus_tag="VF182_06500"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6436762.1"
                     /translation="MPSNKIIDSFVEYMLAARQVPLPAEVIQKGKSHILDSLAAVVSG
                     SRLKPGQLGLQHARQQGGKEICSVLGSDFKTTPIVAAFANGMSGHADETDDSNSQLHP
                     GCAIVPAALAIAESQQRSGDAFLRAVILGYDIGFRFHQAFEPRSTSFGATFGASAAAG
                     ALAELDHLQLCYLISYAAQQASGSRAWVGDDDHIEKAFDYAGMPARNGVTAALLVQSG
                     FTGNRDVLEGDQNIIKTYAPCDPSKLLSELGQRFTITSCLVKKYPVGSPMMETVDATL
                     AMLAKQPIEPQEVERVVVRIPASGARTVNNRKMPDVNVQYMVSTILIDGKLTFESAHD
                     YNRFQDSRVQQMKQRVELIPDEELERTGARFQGLVEVYLKDGRTLREHVANCRGRPEN
                     PMNPEEVEGKAAWLLEPVLGKEKADQIIAASRRLESFGSMRELARLLTVD"
     gene            13195..14163
                     /locus_tag="VF182_06505"
     CDS             13195..14163
                     /locus_tag="VF182_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-dihydroxyphthalate decarboxylase"
                     /protein_id="HEX6436763.1"
                     /translation="MAKPQLTIALSHYDRHVPFFDGTAQPDGIDLNILIVGQSDRQRD
                     GEDRHERMLQHSEFDVAELSLSSYLMAKSRGMPFTAIPVFPRRLFSLSQMWVNVDAAV
                     QSPHDLIGKRVGLHSFQNTLATLSKGDLQSEFNVPWREIQWVLSKKDNIEFEPEKGVR
                     IDQLSNGADIGAMLESGEISAMMTPHPPKPVLRGSKKIRRLFADPKAEDLRYFGKNGF
                     FPIMHVVAFKDEVLKNHPEAARSLFDTFERAKKICREFYADPNWSWLAWGRQAFEEEQ
                     KLLGDDPWPYGLEKNRANLERFVGYSLDQGLMTKKMALDELFVPLN"
     gene            complement(14523..16883)
                     /locus_tag="VF182_06510"
     CDS             complement(14523..16883)
                     /locus_tag="VF182_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6436764.1"
                     /translation="MRKRTLIATQQLHTYCSMCTSRCGVVATVEDGVLTQVHADPEHP
                     NGCICVKGAAAPEIVYSPDRLHYPMVRTRPKGDPDPGWTRITWDQALNLAALRLNNIK
                     DTYGAEAVVFSRATTAGTASIDFDGWLQRLANAFGSPNLLTSNHICTWNRRVGSKYTF
                     GTGMPLPDFDQTQCMLIWGINPPATSPAQALRINRARNRGAKLIVIDPRRTTLAAKAD
                     CWLPVRPGADGELAMAMIHVLIEEKLFDDEFTCRWTNGVWLVRCDNHQLLTQRDLQIG
                     GGSNTYMVWNERENVLETMGDNTAAALFGSFTVNLSDGTTVKCQPVLQMLKDKVAAYA
                     PEQSENITMVPADAVRKAVRLFAAEKPSTYCTWVGLEQDCDAMQTNRAVCCFYALTGQ
                     FDERGSNVLFAITPTNPITGRELLPKAQADRRLGIAQHPLGPPTDPGLVQAGAVYQAI
                     LTERPYPIKALVLFGSDPLLGHGDPLHGKAALEAVDFYIHVDTNLNPSASFADLLLPA
                     TTCWEHQALMPSFEMAEDTLNWAQLRPTVAKPVGESRSDMEIIFDLAQRLGLTEPFFN
                     GDLEAALMHQLAPAGISIRELRENPIGVRANVTTRHRKYAEIDARTGEAKGFETPTRK
                     LEIFSTAFAEAGYPPLPVFDTQGDDADPRYPLILTFYRLVQFCDEQHRNVPRLRRSFP
                     EPYVELHPQTAGTEDIKDGEWIILETANGKVKLKAKFNHSLHPAVVATVYGWWQACQL
                     SKLSGYDPFSEDGANANLLIANSDVDPISASVAHRSQRCRIGKVSG"
     gene            complement(17111..18103)
                     /locus_tag="VF182_06515"
     CDS             complement(17111..18103)
                     /locus_tag="VF182_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6436765.1"
                     /translation="MRRRILSVLFTTLCVAITQPASAQTYEQLIAGAKTEGRLVVYAS
                     ATAPQLQMYFAAFNKKYPFIKTEFFRTGKQALLSKVLLEEQAKQRIADVIHTSVIETS
                     IMKKRGALSKYVPLEAAAYPAQYKDPEGLWTSAYASGTLLGFNSRQVKRPEAPKNYED
                     LLNPRWKSAIAIDSNKIEWFAMLLKLKGQAFMEKLAAQQPTVRSGNTLVLQLLAAGEF
                     PVSAGVYEYSVEDLKTKGAPVDWIGLEPVITYTVAVSLPSQPPHPYSAKLFTEWLLSK
                     EGQEVVNQYGRVPIRDDVDSKYGKILKQHKLLMTDVDLGQKEAEVNATFRKLFQ"
     gene            18349..19971
                     /locus_tag="VF182_06520"
     CDS             18349..19971
                     /locus_tag="VF182_06520"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX6436766.1"
                     /translation="MATPEFVYQDPLPIQKDTTRYRLLTKEHVSVTQFEGKEILKIAP
                     EALTYLSNIAIRDVSFLLRTAHLEKVAAILKDPQASDNDRYVALAMLRNADTAAKGIL
                     PFCQDTGTATIVGKKGQQVWTGVKDEEYLARGIYKTYTEENLRYSQTAPMNMYDEVDT
                     GTNLPAQIDLMATDGIEYQFLFVTKGGGSANKTSLYQETKALLNPGSLEKFLTEKMIT
                     LGTAGCPPYHLAFVIGGTSAEACLKTVKLATTGYLDHLPTTGNAHGRAFRDVETEEKL
                     LQAAYKSGYGAQFGGKYFALDVRIIRLPRHGASCPVGMGVSCSADRNVKAKINRDGIW
                     LEELERDPGRLIPEEYRGKVASNVVRVDLNRPMKEILAQLSKHNVTTQLSLTGTVIVA
                     RDIAHAKIKERVDRGEGLPQYLKDHPVYYAGPAKTPEGMPSGSFGPTTAGRMDSYVDL
                     FQSHGGSMIMLAKGNRSQQVTDACKKYGGFYLGSIGGPAAILAKENIKKVEVLEYPEL
                     GMEAVWKIEVEDFPAFILVDDKGNDFFKQILH"
     gene            complement(19993..21075)
                     /locus_tag="VF182_06525"
     CDS             complement(19993..21075)
                     /locus_tag="VF182_06525"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6436767.1"
                     /translation="MRIIDADGHVLERDIPWADLLEEPYRSRAPRPVKDNRGFNFVMI
                     DGKLTPKPVGKACSFVGAPRSHHPQPTTGMVDPKQRLKDMDLEGIDTAVLFGTSPFLS
                     LPFVEDKDLACAIARVYNNWLADYCKADLRRLKGVALTAIQDPAEAIKELRRAVEELK
                     FVAVATPPISASGKNLDDPDLYPFFAEAERLNIPVCIHVGAGDGVPAGTERFDHPLYT
                     HALAHPFEQMIAALCVVAGGLLEKFPRLKFAFMEAGCGWVPYWMERLDEHYEILQPTV
                     PWLTKRPSEYMRSGQMFYAFEMDEKMLPYVAGFVGADRLVFATDYNHSDSKFPHTVEE
                     VLERTDISESLKAKIMGENAAKLYNL"
     gene            complement(21463..22638)
                     /locus_tag="VF182_06530"
     CDS             complement(21463..22638)
                     /locus_tag="VF182_06530"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX6436768.1"
                     /translation="MDRLNEKIVAFLDPETIEPAAKQQLFNMAELPFVFKHIAVMPDC
                     HLGKGATVGSVIATKGAIIPAAVGVDIGCGMIAVKTKFVANDLPDSLEKTRTGIERRI
                     PLGAGAFNKKLTTSAQRRVAQLNASGRDYQAIDRRWTEALGSLGSGNHFIEISLDESD
                     QVWVVLHSGSRGIGNKLAMKHIKTAQKLMDQNRVQLKDRDLAYLAEGQKEFSDYITDL
                     LWAQDFALLNREEMMDRVMTELSYLFYKEDGHQAEIELERINCHHNFTQRENHFNENV
                     WVTRKGAIQMKQGQKGVIPGSMGTRSYVVSGLENKMAYHSAPHGAGRRFSRGEARRRF
                     TMDDFKKAMAGIECRHSAKLIDELPMAYKDIDEVMENSKDLVKVEHTLRQLVNVKGD"
     gene            complement(22918..>23098)
                     /locus_tag="VF182_06535"
     CDS             complement(22918..>23098)
                     /locus_tag="VF182_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436769.1"
                     /translation="GVKVWNKDMTVDGQAIKLVLEQSTIPKVAELDPSRFFDNTLIRE
                     VNRDYASKLFPGEVK"
BASE COUNT         5222 a         6159 c         6398 g         5319 t
ORIGIN      
        1 aggttccggc aagcgcgccg gtgtaccagc ccaaattcta tccctacgag accggcgaga
       61 aggcttcgta ccgggcgagt tggaacggcg ttccggttgc caccgcggaa atcgaaacga
      121 tacccatcac cttcgaagga aaaaaatctt accgggttcg ggtcgatgcc aaaacttcca
      181 gaggtttgga cttgatttgg aaaatgcgcg acaccatcac ttcagtcttc gatgccaaat
      241 cgctggagcc ctcgcgcttc gttttcagtc aacgcgaaaa ttcccgcgtt atcgacaccc
      301 aggccaagtt caaccaagcg gccaagaaat gggcggtcaa tcgctggcaa gaaggaaaga
      361 agccgcgagt ttacgatttt gactccaaca ataccttgga cccgatcacg gccgtctacc
      421 tggctcgtag cattgacttg aaggtcggtg ataaattgtt tttcaatgtt tttggcggtc
      481 gttatcagta cttgctcgag ttgaatgtgg aaaagaaaga gccggtggag ctggaatccg
      541 gcgccgtcat tcaagcattc aaactcgtgc ccaagttaac caatctgaca aaaaaaggtt
      601 atgcggggcg attgcaggaa gccgctattt gggtttctgc cgatgagcgg cgcattccgg
      661 ttaagttttg gagtagaata tttgccggga atgtctatat ggagttggtt caggatcaga
      721 ccggagtgca atcggctaac agtgtggatt caccggctcc ggcgagttga acaccataga
      781 ggtaagacag cgacgtgaga cgatgaagtg aaaacggcaa aaacttccag gcgctcattt
      841 acgttgtttg cgttctccct tggagtcctg agcgcagtgt tgtccgggtt taggtacccg
      901 tcggcgcccc ggcatcgcgc cggttcggct acggcatatt cggactccaa ccggcgaggg
      961 gatgcgcaga tcgaggatcg tcttcggcgg acgatactgc cgctgttagg agctatggac
     1021 cggccgtgcc gacccgagga actgcgcgtg cagatcatga acgagccaga aatcaatgca
     1081 gccaatgccg gcggttgcca attcttcgtg accagcggct tgctgcagcg cgccaacgat
     1141 gatcatttgc tgggggtaat ggctcatgag atcgctcatc aagatttggg ccatgccgtt
     1201 aaatcgcaga tgctcggcgc cggtctgagt atcggcgcag cgcttctgca acaaattctc
     1261 ccggcgtcag gcgcaattgc gccgctagcc ggaacactga tcgcccgagg ccatagccga
     1321 actgaggagt atgccgccga ccggcacgcc cttgtgctgc tccgccgcat cggttactcg
     1381 ccggagatca tgatcgatac gctgagctgg attcagcgcg cctccggtga tcaacagccg
     1441 ggcagttttc tctccactca ccccgccttg aatgacagaa tctcggcgct caagaaaact
     1501 ctctaacggc ctacgagaag atacatatcc tttgttccgc tcaaacgtct gcatgcactg
     1561 gctagttagg cccgggctct gagatgtttg agcagcctgc aaattgagtt tatcaagctt
     1621 tttcagaggc caacggctcg accggacgcg aactgtctcc acgtcagtcc tgtgtctttg
     1681 cggcgctgac aattgatgta aacgaagtgc tgcggcgatc gtctaagtgg caaaaggaga
     1741 gaatccggga tgaacataaa cctcaaagca atgaaaaaac taaacggcat cgctgccgta
     1801 gcaattttga gcggaacgtt gttagccggc tgcgcggcgg gcggcctcac cacacgtgag
     1861 aaaggcgcag gcattggcgc cctcggcggc gcggccgccg gcgggctcat cggttccgca
     1921 gtgcgtcatc cgggtgcggg cgcagctatt ggagcggcac ttggcctcgg cgcgggggcg
     1981 gtgatcggtg atcaattaca gggccgcgaa aatcagtcct atgaacatga tcagcagatc
     2041 taccgcaatc agcaggaaat caaccgccag cgcgagcaac tcgaagagat tcgccggcgt
     2101 gacaacgagt actactaggc tcgagcatca gtggggcggc actgggttat ctatgtggaa
     2161 caccttctcg caccaatggg actatctcgt gctcaacttg atcgtaggtg tctacatgat
     2221 gctcggtgcc gctctgggat tgaccttgat gtggcttttt ctcacagctc ccactccgta
     2281 gaaacccgct aaccgtgccg cgcattggct cgcgctttcc ttcccgctta agtttcgccg
     2341 ccgctcttga aggccttaga gccccgttct acattctagc tcgtgaaaat cctcgtcacc
     2401 ggcgcagctg gtctgattgg cagccatttc gcgcggcgat ttggcgccgc tcgtgatgtg
     2461 ctcgctttga cacgaaagga tctcgacatt accgatcagg cggcggtgcg aagttgcgtt
     2521 gccgcagaac gaccatctct gctggttaat tgcgcggtga ttcaggtcga cgaagcggag
     2581 cagaatcacg ccaaagctgc ggcagtgaac gtggaagggg cgaggtttct cgccgccgcg
     2641 gcggctcagt atagcgccga gatcattcaa ttcagcaccc aatatgcttt tgacggcgaa
     2701 atcattggac gcgcgccgta cacgatccga gaccagccta ggccgatcaa tttctacggc
     2761 aggaccaagg ttgccgctga agaagtggtg cgcgctgctt gtgcgcaaag ctatatcgtc
     2821 cgcacttcgt gggtttacgg tgaggggaag aacagctttc tttgcacggt gcacaacgat
     2881 ctcggcgcgg gcaagcgggt tcgcgccatc gacgatgttt ggtcgagcac gacctatgtt
     2941 gacgatctca ccgatcgttg cctggagatt ctcaagcttc gccgttacgg tacttaccac
     3001 atcgtcaatg agggcgtttg ttcgtactac gattttgctc tcgaagcggg acgcctgaag
     3061 ggacttggaa aagagcggct cgggtctttg atagaagttg tccaggagcg cgatctgcaa
     3121 agggtcgcgc tccgtcctcg ctacacgccg atgcgttgct tgctctcgga agagttgggc
     3181 ttggcgccga tgcgccattg gcgtgatgcg ctggcgcatt atgtaaaaaa ttagcggatc
     3241 acccgttgtt tgcggccgtt ctgctgccga gatgcattgt cccgcagatt acccgacaag
     3301 ccgcctggca gctttccgcc gggcctgaga atcccgttgc gcccctttcg actggcaaac
     3361 aacctcccaa agttctgagc aggcactgtc gataagcaac agccggatgt acgaagacgg
     3421 ctcagcggcg tagaaagcct aatggagtga tctggccgcg tacaagcgtt gacatggaat
     3481 aaggaccgca gtaaaccggg aagccgaact accatgaaac ctttcttgct tttgaccagt
     3541 gtgttttttc tcgtcgcgtt ttctcgtagc gccccagcgc aaaaattgac cttggcgcat
     3601 gtggcgatcc atcctggcca aggtatgttc tgggtcgcta aagactccgg gttgcttgcc
     3661 aagcacggct ttagtgccga aatcgttctg attcccggga gtcctcgaac ggttcaggct
     3721 ctgattgccg gagatctcga ttatgccgtt gccggcgcgc cggcctatgt gcgcgcccgc
     3781 acgcaaggcg ccgatgtcgt gatcctgtca tcactgtcgg gatattcttc gcaaagagtt
     3841 ctgctcaaac ccgattcaac cgtgcagacg ttcaacgaca tacgagggaa gaccatcggc
     3901 gtcacccagt atggttctgc gggtgattcc ttcatccgca gcgcgcttaa gaaagcggga
     3961 attaaagaaa ccgaagtcac gatcctgcag atgggcggca cgcccggagt atcgcaggcg
     4021 ctggaagcgg gcaggatcga ggtcggcgtg cttggcgagt cgggcgcgct cctcgtcttt
     4081 cgcggaaaag cgcgatttct taaaggcggt agctcaaaag aatttggttt tcatgcgctc
     4141 gatgccccgc tcggcactac cgagcgtaag atcaaagcgg atcgaggttc ggtgctgagg
     4201 ttcatgcaag cctatgtcga ggcaattcat tatttcaaaa gcaatcgagc agggacgata
     4261 aaaatcctcc agaaatatat ggcaggcttg agcgaagagc atgtggcgct ctggtacgac
     4321 gattatcgcg agggattggt atcgctgcca taccccgatg ataacggttt gcgcgcggaa
     4381 ctggacctga tcaacgtgcc gcgctcgcag tcgctcgcgg cctatgtgaa caagagtttt
     4441 ctggacgaga ttaaaaacag cggactggta gaacggttaa acaaataacg gaggacagaa
     4501 ttatggctaa aatcagacat ctcgccatca aagcggaaga ccaagaacgg acggcaacgt
     4561 tttataaaga aacctttggc atgagcgaag cctggcgcgg tccagtgcga gaagacggcc
     4621 atcgcgcaat ctacctcacc gatggatata tcaacatggc aatcctgccg gcgcgtggcg
     4681 ggcgtgaggg aatcgatcac ttcggttttc aggttgaaga tctggaaggg accttaaaga
     4741 cggccgcgtc cacgggcgcg aaaagcgatt cgtacaaaaa accacgggat ggtagattcg
     4801 cggaaatggg cgtgcaagat ccggtgggga ctcccgtgga tgtttccgtg cacgggtgga
     4861 aagcgtagac agcagcgagg tttatcttga gtatgatgat gttgcactgg atggtattca
     4921 ataggggacg tcggcgatga cgaaaacaat ttggttaacc gtcgttctac ttcacgtttt
     4981 cgtgatgccg gcctgtccga gccttgccgc cgaaccttcg aagctattga tcgcccacgg
     5041 cgccatcagc aacaatgttg cgccgttgtg gattgccaag gagcagggga tatttcgcaa
     5101 atacggcata gaggcggatc tgatatttat catcgccggt cgagcgatgc aggcgatgtt
     5161 ggcggggcag gtgccggtgg gcttggttgg cggcacccac gtgaccaacg ccaacacggg
     5221 cggcggggac ttcgcgatga tcctcggcct tgaaaacaga ctcgattaca tttttgtagc
     5281 caagccaacg atcaaaggcg ccgaggatct aaaggggaag aaagtcgcga tcggcacgcc
     5341 ggccggctcg gcatctttgg cgacctatgt gtcactcgat catctcgggc tcgtgccgcg
     5401 ccgcgaccaa atcactttat tgggcatcgg cggcgtgccg gaacgaatgg gcgcgctccg
     5461 gtccggcagt gtggaagcaa ccagcttgtc tcccgaaatt ggccaagtgg tggtcagtga
     5521 aggctatcgg gttttggtcg atctaggaaa agaggatgtt ccgtttcagt cgtccgggct
     5581 ggtcgtgccg cgcagtttga tcaaaaccaa cccccaatta gtagaaaaca tcgccaaggc
     5641 caccgtcgaa ggcgcagctt ttgttcttaa gcccgccaat aaggagattg tcttaagaag
     5701 cctgggccga aatttgcgcc tcgataaacc cgaccgggtc gaaaaggcat atcaaggctt
     5761 gctggcaaat cttccgcgtc caccctgccc gtcgctggag ggaattagcg caatgctcaa
     5821 gctcatggcg cagcacgggc tcaatgccaa ggcgtcacag atcaagcccg aagaaatcgc
     5881 cgacatgagc tactgcaaaa agttcgaaga gagcggattt ttccgtagtc tgtattagct
     5941 tggatccgca gttcaaagtg acgcgacgat caaacggcga accctccaga gcgaaatgca
     6001 atgggtccgt agtttggcgc ggccagcaat ccccggtggt gcagcggact cccctggaca
     6061 aattacgaac cctggaccaa gcacctataa cagcattgaa attgttcgct tctgtgactt
     6121 cgctcctccg gactcgccgc ttgatcagtc cacatcgtta atccttactg cgaatccagg
     6181 attaacttca tcaagaacgg tatcgccaga gttgcggaat ggcgatgcaa attgccgcga
     6241 tcatgatgaa acagagaagg ctgcccagga gcagcgcatt gggtgctccg acataatgtg
     6301 ccgccacacc tgctagaatc gctcccagcc cattggtcgc ctgcgcgaag accgagatca
     6361 ggctcgaggc tcgtccaagc attccatccg gggcctgaag ttgaaccacc gtgcggcgga
     6421 tcgccacgct gaccgagtcg gtgaatccta aaatcaaagt ggcgcccacc gccatccaaa
     6481 accactgagc cactcccagg aacgccaagc tgccggcaaa gatcagcgcc gccgccacca
     6541 ccaaaacgcc tttccgtgct atatcgccca gcatgagcaa ggccaaagag ccgacgattg
     6601 cgcccaagga cggggcgccg tagagcagcc ctaagccgga ggcgccagtg tgaaaaacat
     6661 cggctgcaaa gatcggcaga atcggccggt aaaaacccac cagcgtcacg ccaaaatcga
     6721 gaagaaaaag cgatagtata atgcgctgca cccagatgaa ctcgatgcct tcaaaaatac
     6781 tccgcaggtt gatcgttcgg cgcacgcctt cgggcactcc ggaagtgcgc atcgccacaa
     6841 tcgcaatcgc cgccggaagc agcatcgcat tggtgatgaa ataagtcgtc gataaaccga
     6901 cgcgatcgat caagatcccg ccgattgccg gtccgagtaa aaagctcatt tggatgatga
     6961 tggtattgag ggtcactgcg ttcatgagca acggccgggg caccaatcga gggatcaagg
     7021 cagagcgcgc cggccagtta aaaacctcca ccagggcgcc gagaaatcct aacgtataga
     7081 catgccaagc ttggatgctg ccggtgacgg tgaggattcc taagagcagg cttgggataa
     7141 actggatcga caccgttgcg atgaggagct tcttgcggtc gaaagtgtcg gcaaccgcgc
     7201 cggcaaacag tccgaagatt acgaaaggaa aagcttggaa aaatccagtg agccccaatt
     7261 gaaggggaga accggaaatt tcatacacct gatatagacc agcgacatta cgcacctgaa
     7321 ccgctaccgc attaaagaca ctggcggacc ataccaaacg gtaatcccga atcagcagcg
     7381 acgaccaggg acgaatgttg cttgcaggag atggcaattc tatttcgggt gaatcaggca
     7441 atcggttcag aagtcaaggt gcacggtgcc ggaaatagtt ttgaaatggt ttgctgtggt
     7501 ggtcggcgga tagggcctct agcaggctgg tgaaaaagac cccataggtt ttgaaaagat
     7561 gaattatgaa agctgaaagt tcaattccgc tttcagattt ccgctttcac ccttccaatt
     7621 cgcgcgccac tgctctgaga tcgattgagc agcctgcgac ccgggttttt catcaaccta
     7681 ctagaaaaac cacgccctat ccacggtttg tctatctggt agatgctgct agccgtatgt
     7741 cttccaaaac gccgcgctct tctgcttgcg ccagcaccgt ctcaatgctc ttgaccaaat
     7801 tgcgcgcagc ctcgatagtt aactccacag ccacacgcgc ggccgggcct tgggcctcgt
     7861 tgacaaaatc gatattgagc gcgtgttcca gcggcatgtc atagggatga tcgtaggaga
     7921 tgttggcctc gctgagctcg aaccagtcgc cgccgttctt accgcgccct tcgatcttgg
     7981 cctgatgaac aatcatcgtg cacattttta tttcctctca attcagttcg tcgcactatc
     8041 gtccgagata acagcataac cggcttcgat gtgcggcgcg acgtaaactt tgtcgaagtc
     8101 ttcgatcagc ccttcaaatt tttctcaaga aacccaagaa cttttttcca gccgtcgacc
     8161 gcctgttctt ggcgataggc tggacggtca taatagaaaa acccatgccc ggcgccatca
     8221 tagcggtgaa attcatagct cttgccatgc tttttaagct cggcctcgtg ttggttcacc
     8281 tgttcaggag agggcgattt gtcgtcgttg ccgaagatgc cgatcatttt gcaggaaagg
     8341 tccttggtga aatcgatggg cgccaccgga cgtttaggag tgagttcttc ttgtttcata
     8401 accacgccgc caccccaaca ttccaccaga gcatcgaagc ctttgagctt gcacgcggcg
     8461 aggaagccat gccgcccgcc cgagcaggtg ccaaaaacgc ccaccttgct gacgtaaggc
     8521 aatgaacgga gcagattttt cgcgccatcc aaatctccca aaacctgctc gtcgggtact
     8581 ccgccggccg cgcgtacttt tgcgccgaca tcttccgggc tgccatggcc ttcgcggaaa
     8641 tacaaattcg gtgagatcga gacgtaacca tggtgagcaa acttgcgagt gcattcgcga
     8701 taccactcat cccatccggg cgcatggtgg atcagaacca tgcctggaaa aggccccgcg
     8761 cctaaggggc gggccacgta agcgttgatc gcttcgcctt tgtgcccctg catgctcacg
     8821 gtttcggcga tcatgccttc atattgatct gttcggtaca tattgtcctc cttcaagtcg
     8881 catattcttg aaattgccca gccgtccact taatacaaaa agtatctttg gcgcaactaa
     8941 accgttaaaa aattcctctt gttttggatt tggaaaccca gtaggctaaa agcacacttc
     9001 agtatggagc cttctgtagt gacaaatatc agagagttga aggcttggtc agtctttcct
     9061 tgcgattctt acctcgcgga caatccggcc agagcgtgtt tttcttttca tgttctgatt
     9121 cctgcgttga tcaggttctc agggtcaaag acagcaacaa cgttttccag aggaggagaa
     9181 catgagcagt aagatctatg tcggtggatt gccatactcc acgaccgacg atcagttgca
     9241 ggagattttt tccgcgcatg gagctgtcga atctgctcgg gtgatcaccg acaagttcag
     9301 tggtcgttcg cgggggtttg gatttgtcga aatgagttcc tcagacgagg cgcagaaggc
     9361 gatccaagcg cttaacggca gcgatcttgg tggccggact ctgacggtca acgaagcgcg
     9421 tccccaagaa cggcgctccg gcggtggctt cggtggtggt ggcggcggtg gcggcggagg
     9481 tggcgagagg cgcggcggtg ggggcggacg gaatcgctgg taagtttgta aatcatcact
     9541 tcaagaaagg gcgccaggcc ggggcgccct ttctttttct gggaactctc ttttcattcg
     9601 tcttcactgc tactagctaa atcgtacaac gacagatctc ccttcatcga ttaaacccgg
     9661 attccgtagt tcaaagtgac acagccctca agctgcaagc ctttagagcg aatgcaggat
     9721 cagccgctat agcggtccca gtcctcgcta cgctaggaat ttcaggtgtc agatttgcca
     9781 tttgagattt gcgatgccgc agagcggcct tgctcctccg ggcgcccttg atcagtacac
     9841 attgttaatc tctacaggaa tccgagttaa agattccgat cggatgctgc tgaactattc
     9901 actctacgag tttcagtatc tgaacatccg ccgggtacga gtgatctgct gatagctaac
     9961 gatcggcgag aatgtgatac cagaggagca tggccgccca gcgagagcta cttggattta
    10021 taggacgcgt gctttagttg aaccttttca tcgggcggcg gggtccaaac aagatcgccg
    10081 cgatgagcat agatatccat gctgttccac agtggaattg ccgctgcatc ttctaaaatg
    10141 attctatgag cgtctcccag cagccgttct cgtttcttga catcgaaggt ttgttgctcg
    10201 gtatcgagga tctcgtccag cttgggattg ctgtatccga tcacacgctt ggtcacaccg
    10261 ctcctgaaat attgatgaaa tagcgcattc gcatcggtga ggctgccgcg gccggtcaga
    10321 tacatcggat acttgccgtt tttgtaatcg gtgttgaaga tgctccattc gggtgttttg
    10381 agaatcgctt tgatgcctac ctttgaaagc tgctcgacaa tcacttgtgc gatctcgagg
    10441 tcttttggat atcgtcccgt aggactgtag tactcgatct caaagccatt gccaaacccg
    10501 gcttctttta aaagttgttt cgaattctgg acgtcgtaag ggtaagcctt cgctccggga
    10561 tcatacccga agacctgtgg gctgagcagg cctaacaatg gataggcgtt cccctctaag
    10621 atgtgtttga tcaatccttc tcggtcgatc gcctgtgtaa tcgcgcgacg gaccttcggg
    10681 ttatccagcg gtttgtaagc cggactcaat accagaaaga tgggccgcag cccctgcact
    10741 ggttgcacct ggagtcgtgg attggattta agtcgtgcta cttcatgagg cggtatattg
    10801 ctgatcaaat ccgcttgccc ggattcgagc gctgcaatgc gagcggcatc ttctttgata
    10861 actttataaa gaatctcctc gatttttgcc ggtggtcccc agtaattttc attgcgtcgc
    10921 atgacgaagt tgccggaacg ctcccaactg acaaatttga aagggccggt cccgattggc
    10981 ggattgtctg cctctcccat cttttcagcg acggcctgct tcatgatcac ccggttattt
    11041 agatcctcga gagccgttgc gtccgccttc ttgagtgtca ggacgaaact aaagtcatcg
    11101 ggtaactcca tttccttgat gttctgtaag cttggcgcta ccaagctttg cttgtcggtg
    11161 atcatccgct tcaacgaagc catgacgtcc ttgccggtaa acggagttcc gtcgtgaaag
    11221 cgaacccctt tgcgaattgc gaaatggagc ttgttcccct tgacggtcca ggattcagcc
    11281 aggatccccc tgtattcacg tttgtcgtaa tcgaagtcaa tcagcgtttc catcaccgat
    11341 ccccaaactc cctggatcgg ggatgtggtt accgcatatg gattcaggga ataaacgccg
    11401 actccgtggg ccacggtgac cctattctta ttgagggact gagcttctgc gatgggtgaa
    11461 atggcatgga aatgtgagaa ggagaggact gcgctggccg ctgctgattt tttgatgaat
    11521 tccctgcgac tccagcatct acttttcgtg ctcacagaaa cctcctctgt tgtgaagtgc
    11581 cgatccttca gttaaggtct gccgagcgaa cgaggaaact aaacacgcat ataacgtatg
    11641 cgatcaaata gtgtcaactt gttcttgaaa gccgttctcg agtaacccac gatcctctca
    11701 gctttcgtag tggaaaaaaa gtagcaatcg gcaacggagt gtagcaatcg gcaacggagt
    11761 attgatatct ggaggagcgc atgccgtcta acaaaattat agattcattt gttgagtaca
    11821 tgctggctgc acgacaagtg ccgctgcccg cggaggtgat ccaaaagggc aaaagccata
    11881 ttctcgattc gttggccgct gttgtatcgg gttcccgcct gaagccagga cagctggggc
    11941 tgcagcacgc tcgtcaacaa ggaggcaaag agatttgcag cgttttaggt tccgatttta
    12001 aaacgacacc gatcgttgcc gcattcgcca atgggatgtc gggtcatgcc gacgaaacag
    12061 acgattcgaa cagccaactg caccctggct gcgcgatcgt accggccgcg ttggccattg
    12121 ccgagtcgca acagcgtagc ggtgatgctt tcttgcgtgc ggtcatttta ggatatgata
    12181 tcggattccg cttccatcaa gcattcgagc ctcgatccac gagttttggc gcgacgtttg
    12241 gcgcgtctgc cgcagccggt gccctggccg aactcgatca cttgcaactt tgttacttga
    12301 tctcttacgc ggcgcagcaa gcgtcgggtt cccgcgcttg ggttggtgac gacgatcaca
    12361 tcgaaaaggc attcgactat gcgggcatgc cggcgcgtaa cggcgtaacc gctgcgctgc
    12421 tcgtccagtc gggattcacg ggcaatcgtg acgtgctgga gggagaccag aacatcatca
    12481 agacttacgc gccgtgcgat ccgagtaaac tgttgagtga actcgggcaa agattcacga
    12541 tcaccagctg cctggtcaaa aaatatccgg tgggttcacc gatgatggaa accgtcgatg
    12601 cgacgctggc tatgctcgcc aagcagccga ttgaaccgca ggaagtggag agagttgtcg
    12661 tgcgcatacc tgcgagcggc gcgcgcacgg ttaacaaccg aaagatgcct gatgtgaacg
    12721 tgcagtatat ggtttcgacc atcttgatag acggtaaact cactttcgaa tcagcgcatg
    12781 attacaatcg tttccaggat tcgcgcgtcc agcagatgaa acagagggta gaattgattc
    12841 cagacgaaga gctggagcga accggcgccc ggtttcaggg gctagtcgag gtttacctga
    12901 aagacggtcg aaccttgcgc gagcacgtcg ccaattgccg cggccgcccg gaaaatccga
    12961 tgaatcccga agaggtggag gggaaagccg cttggttgct ggagcccgtc ctgggcaagg
    13021 agaaggcgga tcagatcatt gcagcgagcc gtcggttgga atctttcggc agcatgcgtg
    13081 aattggcccg gctgctaacc gtcgattgat gtttgattgt tcgtcgttca tctatccggg
    13141 ctgagttttg cgtgttcgat atcagcgaat caactcgtct tcgaaaagaa cctcatggca
    13201 aagcctcaat taacaatcgc gctgtcccat tatgatcgtc atgtgccttt tttcgacggc
    13261 acggcgcagc ctgacggcat cgacctcaac atcttgatcg tcggtcaatc cgatcgacag
    13321 cgggacggcg aagatcgtca tgagcggatg ttgcagcatt ctgagttcga cgtggccgag
    13381 ctctcgctat cctcttatct aatggccaag agccgcggta tgccgttcac cgcgattccg
    13441 gtgtttccgc gccgcttgtt cagtctttca caaatgtggg tgaatgtgga tgcggcggtt
    13501 cagtcgccgc atgatctgat cggcaagcga gttggcttgc acagttttca aaatactttg
    13561 gcgacactct ccaaaggcga cttacaaagc gaattcaacg tcccgtggcg ggaaatacaa
    13621 tgggtcctca gcaagaagga caacatagaa ttcgagccgg aaaagggcgt caggatcgat
    13681 cagttgtcga atggcgccga tatcggcgcc atgctcgaat caggcgagat cagcgcgatg
    13741 atgacaccgc atccgcccaa gccggtgctg cgcggctcga aaaaaatccg ccgcctgttt
    13801 gccgatccga aagcggagga tctcagatat tttggcaaaa atggattttt cccgatcatg
    13861 catgtggtgg cgttcaagga cgaggtgttg aaaaatcatc ctgaagcggc gcgcagcctt
    13921 ttcgatacat tcgaacgagc aaagaagatc tgccgtgaat tctacgccga ccctaactgg
    13981 tcgtggctgg cctggggacg tcaggcattt gaagaagaac aaaaactttt gggagacgat
    14041 ccctggccct atggcctcga aaagaaccgc gccaacctgg aacggttcgt tggctactcg
    14101 ctggatcaag gattgatgac caagaaaatg gcgctggacg aactctttgt gccgctgaac
    14161 tgacaaatca atgaagctcg acgttacacc taagggagtc gcacaatctt ttaagcggtg
    14221 caacatcgct gcgatcgaag cctgatttct caggctaaag tgacacgtcg atcaccaggt
    14281 aagccctcca gagtgaatgc aatgggccag cggctcgtgg caatctgcga tcccgggcac
    14341 gccagcggac tcccagcgaa attgccggca ctggaccaag caccctaacg acattgaaag
    14401 agtttggaaa tagtttgcgt ctgcgcgttc gctcctccag gcctacgtct tgatcggtac
    14461 acaggtgtca atcccaactg cggaatcgga tttaaaaagc cgcttcctgc ccgaaacagc
    14521 cgctaaccag agaccttccc gatgcgacat ctctgcgagc gatgcgcaac cgatgcgcta
    14581 atcgggtcca cgtccgagtt ggcaatgagg aggttggcat tggcgccgtc ttcactgaat
    14641 gggtcgtaac cgctgagctt cgataattga caggcttgcc accagccgta cacggtggcc
    14701 acgaccgccg gatgcaacga gtggttgaat ttggccttta gttttacctt tccgtttgcg
    14761 gtttccagaa taatccattc accgtctttg atgtcttcgg ttccagccgt ctgcggatga
    14821 agctcgacat acggctcggg aaacgatcgc cgcaggcgcg ggacgttgcg gtgttgttcg
    14881 tcgcagaact gcactaggcg gtaaaatgtc aaaatcagcg gatagcgcgg atctgcatcg
    14941 tcgccttgag tgtcgaaaac gggtagcgga gggtagccag cttcggcgaa ggccgtcgaa
    15001 aaaatttcaa gctttcgcgt cggcgtttcg aaacctttcg cttcgccggt gcgcgcatcg
    15061 atctccgcat atttgcggtg cctggtagtg acattggctc gaactccaat tggattttca
    15121 cgcagttccc ggatggaaat ccccgccggg gcgagttgat gcatgagcgc tgcttcaagg
    15181 tcgccattga agaacggttc ggttaacccg agccgctgcg ctagatcgaa aataatttcc
    15241 atgtctgatc gtgactcgcc cacgggtttt gccaccgtcg gccggagctg ggcccaattg
    15301 agtgtgtcct cagccatttc gaaagagggc atcaaggctt gatgttccca acaggtggtt
    15361 gccggcagca acaagtctgc gaatgatgcg ctcgggttaa gattggtgtc aacgtggatg
    15421 tagaaatcaa ccgcttccag cgcggctttg ccatggagcg gatcgccgtg tccgagcagt
    15481 ggatcgctgc cgaacaatac gagagccttg atcggatacg gacgctcagt gagaatggcc
    15541 tgatagactg cccccgcctg caccaatccc ggatcggtgg gcgggccgag cggatgctgt
    15601 gcgattccca gccggcgatc cgcttgcgct ttcggcaata gctcacggcc ggtgatcgga
    15661 ttggtaggcg taatggcgaa gagaacgttg ctgccgcgct catcgaattg ccccgtcagc
    15721 gcgtaaaaac agcagacagc ccgattggtt tgcatagcgt cgcagtcttg ttcgagcccg
    15781 acccatgtgc agtaggtgga cggtttttcc gccgcgaaca ggcgcacggc ctttctgact
    15841 gcgtcagccg gaaccatggt tatgttttcc gattgttcgg gtgcatatgc ggcgactttg
    15901 tctttgagca tttgaagcac cggttgacac ttgacggttg ttccgtcgct gagattcacg
    15961 gtgaaactgc caaaaagcgc cgcggcagta ttgtctccca ttgtttcgag cacgttttct
    16021 cgttcgttcc aaaccatgta ggtgttcgaa cctccaccaa tctgcagatc acgctgcgtg
    16081 agaagttgat gattgtcgca gcgaaccagc cacactccgt tggtccagcg gcaggtgaac
    16141 tcatcgtcga aaagtttttc ttcgatgaga acgtggatca tcgccatggc cagctcgccg
    16201 tcggctcccg gacgaaccgg caaccagcaa tcggctttgg ccgccaatgt cgttctgcgc
    16261 ggatcgatga cgatcagttt cgcgccgcga tttcgcgccc ggttgatgcg cagcgcttgc
    16321 gccggtgaag tggcaggcgg attgatgccc cagatcaaca tgcactgagt ctggtcgaag
    16381 tccggcagcg gcatgccggt gccgaaagtg tacttcgatc cgacccggcg gttccaggtg
    16441 cagatatgat tcgaggtgag gaggttgggg ctgccaaagg cattggccag ccgttgcagc
    16501 cagccatcga aatcaatcga tgcggtgccg gcggtggtcg cgcgggaaaa gaccacagct
    16561 tcggcgccgt aggtatcctt tatgttatta agtcgcaacg cggcgaggtt gagggcctgg
    16621 tcccaggtga tgcgagtcca accgggatca ggatcgccct tgggtctggt tcgcaccatt
    16681 ggataatgta ggcggtccgg cgagtaaacg atttccggcg cggccgcgcc cttgacgcag
    16741 atacaaccat tgggatgctc agggtcggca tggacctgcg tcagcacccc gtcctcgacg
    16801 gttgcgacca cgccgcaacg cgacgtgcac atcgagcagt aggtgtgtaa ttgttgcgtg
    16861 gcgataagag ttctttttcg aattggttat ctagccaact aagcttcgtg agggcacccg
    16921 agccctacag ccgtgggcaa tgggacagtt ccttgaaggc cgccccgcat ttcccctaag
    16981 ggtgatcttt cgtacgtcga gcccttatag tcaactcgcg ttcaggtgcc cacggcttcc
    17041 gatctcgccg tcctcgctca ctgctgacgg tgcaaactat tgtcggatta gggtttaact
    17101 caaccggatt tcactggaat agttttcgaa atgtagcgtt gacttcggct tccttctggc
    17161 ctagatcgac gtcggtcatc agcagcttgt gttgtttgag aatcttgccg tacttagagt
    17221 ccacgtcatc acgaatcggc acccggccat attgattgac gacctcctgg ccttcttttg
    17281 acaacaacca ctcggtaaaa agtttcgccg aatacggatg aggcggttgc gacggcagcg
    17341 agacggcaac ggtataggtg atcaccggtt ccaagccgat ccagtccacc ggcgctcctt
    17401 tggtcttcag gtcctccacg gaatattcgt acacgccggc ggaaaccgga aactcgcccg
    17461 cagccagtag ttgtaagacc aaggtgttgc cggagcggac ggtgggttgc tgggcggcaa
    17521 gtttctccat gaacgcttgg cccttgagct tcagcagcat agcgaaccac tcgattttgt
    17581 tggaatcgat agcgatggcg cttttccagc gcggattcaa taaatcttcg taattcttgg
    17641 gcgcctcggg ccgtttaact tgccgcgaat taaaaccgag cagggtgccg ctggcgtagg
    17701 cagatgtcca aaggccttcc ggatccttgt actgcgccgg ataagcggcg gcctcgagcg
    17761 gcacatattt gctaagggcg ccgcgctttt tcatgatgct ggtttcgatg acgctggtgt
    17821 ggatgacgtc ggcgattcgt tgtttggctt gctcttcgag gaggacctta ctcaacagcg
    17881 cttgtttgcc ggtgcgaaag aattccgtct tgatgaaagg atattttttg ttaaatgcgg
    17941 cgaaatacat ctgtagctgc ggcgcggtgg ccgatgcata gacgaccagt ctgccttccg
    18001 ttttggcgcc ggcaatgagc tgctcatagg tttgcgcgga ggcaggctgg gtgatggcaa
    18061 cacacaacgt agtaaaaaga accgatagga tccttcttct catgcgccct ccattatcaa
    18121 agaaagtagt cgagcaggcg ctgatctgtc aacggtctat gaccgggcaa cgcgaatgtc
    18181 ctttgctgtc acttcgacca gagaagaccc gatttgtcgc cggggtaact gctgcggagt
    18241 tcgcgcgcga attttacgca aggcatatct ggcaaggcga tctcgtcaat taccgtacgt
    18301 cgacaagctg gtattttttg gctactattt gtaatcgagg tgtgaaaaat ggcgactccc
    18361 gaatttgtct atcaagatcc gttgccgatt cagaaagaca cgaccagata ccgcctgcta
    18421 accaaggagc atgtctctgt cactcaattc gaaggcaagg aaatactaaa gattgcgccc
    18481 gaagcgctca cctatctatc gaatatcgcc attcgggatg tatctttctt attgcgaacc
    18541 gcccatcttg aaaaggttgc ggcgatcctc aaggacccgc aggcttccga caatgatcgc
    18601 tatgtcgcct tggcgatgct gcgcaacgcc gacaccgcag ccaaaggcat tttgccgttc
    18661 tgccaggaca cgggtacggc cacgatcgtc ggcaaaaaag gccagcaggt ctggaccggt
    18721 gtgaaggacg aagaatacct ggccagaggg atctacaaaa cgtacaccga agaaaatctg
    18781 cgttactccc aaaccgcgcc catgaacatg tacgacgagg tcgataccgg caccaatttg
    18841 ccggcgcaaa tcgatctcat ggctaccgac ggcatcgagt accagttttt gttcgtcacc
    18901 aaggggggtg gatcggctaa caaaacttcg ctgtaccagg aaaccaaggc cctactgaat
    18961 ccggggagct tggaaaaatt tctgacggaa aaaatgataa cgttggggac cgccggttgc
    19021 ccgccatatc atctggcgtt cgtcatcggc ggaacttcgg ccgaagcgtg tctcaaaacc
    19081 gtgaaacttg cgaccaccgg ctatctcgat catctgccga ccaccggcaa tgctcacggg
    19141 cgcgccttcc gcgatgtcga gaccgaggag aagctcttgc aggccgcgta caagtcaggc
    19201 tacggcgcgc aattcggcgg caagtatttc gcgttggacg tgcgcatcat ccgtttgcct
    19261 cggcatggcg catcctgtcc cgtgggtatg ggagtttcgt gttcagcgga tcgcaacgtc
    19321 aaggcgaaga tcaaccgcga cggcatttgg ctcgaagaat tggaacggga tccgggccgg
    19381 ctgattccgg aagagtatcg cggcaaggtt gccagcaatg tcgtgcgagt cgatctcaac
    19441 cggccgatga aagaaatctt ggctcagctc tcgaagcata acgtaaccac gcagctttca
    19501 cttaccggca cggtgatcgt tgctcgggat atcgcgcacg ctaaaatcaa agaacgcgtg
    19561 gacagaggcg aaggattgcc gcaatatctc aaagaccatc cggtttatta cgccggcccg
    19621 gccaagactc cagaaggaat gccctcgggt tctttcgggc cgactaccgc cggacggatg
    19681 gattcttacg tcgatttgtt tcaatcgcac ggcggatcga tgatcatgct cgccaagggt
    19741 aaccgcagcc agcaggttac cgatgcctgc aagaagtacg gcggctttta tctcggctct
    19801 atcggtgggc cggcggcgat actcgccaag gaaaatatca aaaaagtcga agtgctcgaa
    19861 tatccggagc tgggaatgga agcggtttgg aagatcgaag tcgaagactt cccggctttt
    19921 attttagtcg acgacaaagg caacgacttc ttcaaacaga ttcttcattg agataagttg
    19981 ccgctgccgg gtttacaaat tgtagagctt ggcggcgttt tcacccatga tttttgcctt
    20041 caagctttcc gatatatccg tgcgctcgag cacttcttcc accgtgtgcg gaaacttgga
    20101 atcgctatga ttatagtcgg tggcaaagac taaacgatcg gcgccgacga atcccgcgac
    20161 gtagggcagc atcttctcgt ccatctcgaa ggcatagaac atttggccgc tgcgcatgta
    20221 ttcgctaggc cgtttggtca gccaaggcac cgtcggctgc aggatttcat agtgctcatc
    20281 gagccgctcc atccagtacg gcacccaacc gcagcccgct tccataaagg caaacttcag
    20341 tctgggaaat ttttcgagca acccgccggc gacgacgcac agcgccgcga tcatttgctc
    20401 gaacggatgc gccaaagcgt gagtatagag cggatgatcg aatcgttcag tgccggcggg
    20461 cacgccgtcg cccgcgccaa catgaatgca gacgggaata tttagccgct ccgcttcggc
    20521 aaaaaaaggg tagagatcgg gatcgtccaa attttttccc gacgctgaga tcggcggtgt
    20581 cgccaccgct acgaatttga gttcctctac ggcgcgccgt aattctttta tggcttcggc
    20641 gggatcctgg atcgccgtaa gagcaactcc cttcaggcgc cgtaaatcgg ccttgcaata
    20701 atcagccagc cagttgttat aaacccgcgc gatggcgcaa gccagatctt tatcctccac
    20761 aaaaggaagg ctcaaaaaag gagaagtgcc gaacagcacc gcggtatcga tcccttccag
    20821 atccatgtcc ttaagccgct gtttgggatc gaccatcccg gtcgtaggct gtggatgatg
    20881 gctgcgcggc gcgccgacaa aactgcaggc tttgcccaca ggtttcggcg tgagcttgcc
    20941 gtcgatcatg acaaagttaa aaccgcgatt gtctttgacc ggcctcggag ctcgggagcg
    21001 ataaggctct tccaacaaat ccgcccacgg aatatctctt tcgagtacgt gcccgtccgc
    21061 gtcgatgatg cgcataatcc tacttgcctc ccagcaggct gctgaaaact cattcgcgat
    21121 ggaagatgaa ttatgaaagc tgaaaactga attccgcttt cagatttccg cttttcatcc
    21181 ttgtaattcg ctctcctacg atatttgtga ccagcctgca aataaagttt tagcacatag
    21241 aactagtctg ttcattcctg gcgaagcaac acaatttgct tgcgctcaag cgatatgcat
    21301 ggcgccaaag ttggaaagca tgcccgggat tggctcgaac gctgtagaaa catattcggc
    21361 tggatacttc ctcgtcgctc ggtcgcttag atgttctgct tacatgaact cgaactgttc
    21421 ggctgtgaat ctgaaacggc cggcaagtta tcgattcttt gtctaatcgc cttttacgtt
    21481 gaccagctgt cttaatgtgt gttcgacctt aaccaggtct ttggaatttt ccatcacctc
    21541 atcaatgtcc ttgtaagcca tcggaagctc gtcgatcagc tttgccgagt ggcgacattc
    21601 gattccggcc atagctttct taaagtcatc cattgtgaac cgtcgccgcg cttcaccgcg
    21661 ggagaacctg cggcccgccc catggggcgc tgaatggtaa gccatcttgt tttccagtcc
    21721 tgaaacgacg taggagcgcg ttcccattga tcccgggatt actcctttct gcccttgttt
    21781 catctggatt gcgccttttc tcgtcaccca gacattttcg ttgaaatgat tttcgcgctg
    21841 ggtgaagttg tgatggcaat tgatgcgttc gagctcgatc tctgcctggt gtccgtcctc
    21901 tttataaaac agataagaaa gttcggtcat gacccgatcc atcatttcct cgcgatttaa
    21961 gagagcaaaa tcctgcgccc agagaagatc agttatgtag tcgctgaatt ctttttgccc
    22021 ttcggcaaga tacgccagat ctctgtcctt cagctgcact cgattctggt ccataagttt
    22081 ctgcgctgtt ttgatgtgct tcatcgcgag cttgttgcct atgccgcgcg agccggagtg
    22141 aaggactacc caaacttgat ctgactcatc gagagaaatt tcaatgaagt gattgcctga
    22201 acctagagag ccaagcgctt cggtccatcg tcggtctatg gcttgataat cccttccgga
    22261 agcattgagt tgcgctactc ttctttgggc gcttgtggtg agctttttgt tgaacgcgcc
    22321 cgcgcctagc gggattcgcc gttcgatacc ggttcgtgtt ttctcgaggc tgtccgggag
    22381 atcgttggca acaaacttcg tcttcaccgc aatcattccg cagccaatgt ccacgcctac
    22441 cgcggccgga ataatcgctc ccttggtggc aatgactgag ccaacggttg cgcctttgcc
    22501 gagatggcaa tccggcatga ccgcgatgtg cttaaacacg aaaggcagct ccgccatatt
    22561 gaaaagctgc tgtttagccg caggctcaat cgtctctgga tctaagaagg cgacaatttt
    22621 ttcgtttagc ctgtccatag attcactata acaaaccgtt tggtcgtccg ctgaggataa
    22681 gcttgtctgg tgacgaggcg aaaaggagtt ctggaactca tctgccgggt ttgccttact
    22741 caggtccatc ttttagggct tcgtcggtca aacgttctca ttccgtacgt gccatgcggg
    22801 cgtcaagccg accgggactg ccgcgttaca acacaatttt cgagattggc cttagtgagt
    22861 gaatagctct aaagcgacag agctacgtcg ccgacagcaa cgttcggctc taaacgatta
    22921 cttcacttcg ccaggaaata atttggaagc atagtcgcgg ttgacctcgc ggatgagcgt
    22981 gttgtcgaag aaccggcttg gatcgagttc agcgactttc gggatcgtcg attgttcgag
    23041 caccagcttg atcgcctggc catcgacggt catatccttg ttccacacct tgacgcct
//
LOCUS       DASUVS010000117        14361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1569285, whole genome shotgun sequence.
ACCESSION   DASUVS010000117 DASUVS010000000
VERSION     DASUVS010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14361
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569285"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /locus_tag="VF182_06540"
     CDS             complement(<1..1533)
                     /locus_tag="VF182_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6436770.1"
                     /translation="MRTDTDVDTNRPNDELLDIEKDDTEELEGDEAELELESDGATAE
                     ENAGEGEVQPEEKGLTVDPITTYLHEIGSIPLLSREREVELARKMDIGKNAIIEALFN
                     APMCLHYTIGLGEAVASGELDIREVLEKSDAEEDENRVSFDPKPFLRAISKLKRLRHE
                     QEKVHRQLNQKRVSEQRRNALNKAHASLLNRIVGLLREVGLSSSRLESMVQKLKQSSN
                     TITELEERAHVASKAKRAAIVSQIEQMESGVGLRAAEIHALVSQITATESQVNVAKTE
                     FTEANLRLVVSIAKKYTNRGLSLLDLIQEGNLGLMRAVEKFDYRLGYRFSTYASWWIR
                     QGITRGLIDTGRMIRIPVHRVELRNKVIQTAHHLQRTLGREPKPEELAKEMDMSVPEM
                     LRVMQTQGEPVSLQTPVWEDGDQLADFVEDRINPQPDKEAMEGVLRTDVRKALAVLTP
                     RQETVLRMRFGIEEERDYTLEELGEIFAVTRERIRQIEQKSLQILRNPNRRKPIPPTS
                     DAA"
     gene            2331..3038
                     /locus_tag="VF182_06545"
     CDS             2331..3038
                     /locus_tag="VF182_06545"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6436771.1"
                     /translation="MKSHEWRQELGECVRYLFRSSEMEHFYSIKMTTQRACIYLLQLS
                     LYVRQRRNFWPQVAANCPELEVKQRIMSHEYEELVEDEHSKHGHLDLIFRQGKEVGLS
                     SDKIITAQPLPTTTAAIYAWFWIARNRPWQEAIAASTIAEWTNDDRLLGDLGGGNCSR
                     LYTIWKTDLNLSDGQMPNFTAHSKADVKHSDMFIDVLEKYVAPGREQDVLNTAKESMD
                     IHRAYFGGMAQAMSKAM"
     gene            3054..4070
                     /locus_tag="VF182_06550"
     CDS             3054..4070
                     /locus_tag="VF182_06550"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6436772.1"
                     /translation="MNLAELDSKLAKNQLAGFWNTRVPGHSAEAPYLWKWESLLDGLL
                     KASETIGIEQAERRAIRLISPHVPNKSTSHTIQLTFSIVNPGEVARAHRHNMAAIRFV
                     VQGKGAYTAVGGEKFPMEEGDLVLTPNWTWHEHHNESPDPIIWLDGLDGPLIQALNVL
                     FFEQSEKPEQPITRTTGESLSRLGFARAPKLDETQLRGVPFRYAWQDTYKTLKLMSRN
                     DQDPYDGALLRYINPATGGYTYPTMSCEIQLLNPKQSTRVHRHTSTAVYHVFKGHGRT
                     AVGESCLEWNKGDSFVIPLWQWHSHENPFDEEAILFSINDRPVMEALQLYRAEIRNGN
                     NDAE"
     gene            4169..5170
                     /locus_tag="VF182_06555"
     CDS             4169..5170
                     /locus_tag="VF182_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX6436773.1"
                     /translation="MADTNIKLASRTYDGILPILRGQMQIPGFHLEIAETSDVAGMFA
                     GMFAGRYDVGEMSLGELIYYTSRTRADFVGIPVFPSRMFRHGFIFCRRSSGIDKPGDL
                     SGKSVGFLRWVQTAAIWMRGMLVDEYGVSASATRWYIASIHHWDDADPSATVEPRDGS
                     MIHQIGSTGKSASERACRALFNGEVDALGVTESQLPSLLANDKVGRLFADPREVEASY
                     FRKTRILPIMHVVAIRKKLVEQHPELPGKLFRLYCEAKRWAQQWRRAIPSLVEAWPNH
                     YLSEEQRIFNTDPWAYGLEANRHVLDTFLAYCDAQGVSAKKIAPDELFDPSTLKLVD"
     gene            5202..6695
                     /locus_tag="VF182_06560"
     CDS             5202..6695
                     /locus_tag="VF182_06560"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6436774.1"
                     /translation="MSTALASKSQKVAACDLRDWLRQVDATGQLATISGAHWDLELGA
                     LTEIILERMSTPPAVMFDSVPGYEPNRRVLANMLETLDRTALTLNLPLDLKTIPLIDA
                     LRAKLRALQPVKPNVVSTGPIMENVERADQLDLFKFPVPRWHEGDGGRYLGTAHLVIT
                     RDPETGVENVGCYRVMLQDRDKVGLYISPGKHGRMHYEKAMKAGKPFPVAMVFGQHPL
                     LFIASSQAVPFGVNEYDWTGGILGQPIDVIKGPLTGLHFPASAEIAIEGEIVPGEMLP
                     EGPFGEWPGYYASARRAEPFVRVKALYYRNDPIICGAAPFKPTIHGMYRSCLRAATVW
                     NGMEQAGVPDIRGVYLPPPAQRFMIVVAIKQRYPGHAKQAALMAAQCHAGAYLGRYVI
                     VVDEDIDITNINEVVWAIATRSDPATSVDILRRTWSGPLDPIIQPGQKGHNSRMIIEA
                     VRPYEWRDRFPATSAISDETRKEYSAKWAKELGAIEGRQTRPRAHLV"
     gene            6992..7213
                     /locus_tag="VF182_06565"
     CDS             6992..7213
                     /locus_tag="VF182_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6436775.1"
                     /translation="MEEIMTPSEVANLLKIHLKTVYKLAEKGVIPGNRIGRSWRFSRS
                     DVLELVSSKPINLSDGDTDDQVSRLAAVK"
     gene            7662..8393
                     /locus_tag="VF182_06570"
     CDS             7662..8393
                     /locus_tag="VF182_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436776.1"
                     /translation="MEIFIAVFSGAAFLALIVYLWNTDGASKQGKQEIIEEESLSAES
                     TTEPSPRSLILSATESEQVVALEQKIDAVNPEQAESFKALEEKATAYKSELDQAQASA
                     AHNSQRVVELEADNSLLQRRLTELDAQQRHRQSELASLDDQVAKLGAIEQQVEVLQKE
                     KAELTQHLEQLQIENAANARATLALQDLEAKQSEYEEKLASLGAENRRLLGEITGLQS
                     SLRDKIKTQLDGLQELYRNLTPGLN"
     gene            complement(8513..8587)
                     /locus_tag="VF182_06575"
     tRNA            complement(8513..8587)
                     /locus_tag="VF182_06575"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8552..8554),aa:Arg,seq:ccg)
     gene            8847..9719
                     /gene="ispE"
                     /locus_tag="VF182_06580"
     CDS             8847..9719
                     /gene="ispE"
                     /locus_tag="VF182_06580"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX6436777.1"
                     /translation="MLPVSVKIRAPAKINLGLRVVGKRADGYHLLDTIMVPVSLYDEI
                     HLTKMGEGRGSSSTLTVTCDNPTLPTDEGNIVFRAAHLIIKEKLIREPVAIHIKKRIP
                     IGAGLGGGSSDAAATLMAMNSLFRLNYSPRQLKKRAYRLGADVPFFIDRRPARARGIG
                     ERLTPIKSLAWKWAVIVYPGFPVATSWVYRNLEPKLTKAMANTSIISPLRKSFDQQRL
                     LVNDLESVTVARYPRIRFLKEKLIAEGAAGALMSGSGSAVFGLFDSRGNAARALSRLQ
                     KEDGVETFLVRLLT"
     gene            9792..10079
                     /gene="spoVG"
                     /locus_tag="VF182_06585"
     CDS             9792..10079
                     /gene="spoVG"
                     /locus_tag="VF182_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HEX6436778.1"
                     /translation="MQVTEVRVFPVDEDKLRAYVTITLDHCFVIRDLKIIKGTTGYFV
                     SMPSKKRKDGTYKDIAHPINNETRRMLEEKVIAEYERVAAEGGSQRALGNG"
     gene            10130..10203
                     /locus_tag="VF182_06590"
     tRNA            10130..10203
                     /locus_tag="VF182_06590"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10162..10164,aa:Gln,seq:ttg)
     gene            10385..11338
                     /locus_tag="VF182_06595"
     CDS             10385..11338
                     /locus_tag="VF182_06595"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX6436779.1"
                     /translation="MSQQKIDVKIFAGNSNPPLAREISDYLAVPLGKATVETFSDGES
                     KVEIKENVRGADVFVLQSTSAPGNNHLMELLLMLDAFKRASAKRITAVIPYYGYARQD
                     RKVLPRVPISAKLVADLITTAGASRMLTLDLHSGQIQGFFDIPVDNLYATPVLLQHLK
                     KQLNHNEVTIISPDAGGVERARAIATRLDASLAIIDKRRVGPNVVAEMKIIGEVQDQV
                     AILVDDMVDTAGTLTMAADALKKEGAKRIIGCCTHPVLSGPAIQRIDDSPLEELIVTN
                     TIPLQSEATHCGKIKVLSVAHLIGEAIRRTHEEESISSLFV"
     gene            11360..12004
                     /locus_tag="VF182_06600"
     CDS             11360..12004
                     /locus_tag="VF182_06600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX6436780.1"
                     /translation="METVEIEVNAREPGSKHQARRLRREGKIPAVLYGPKVQPLALEL
                     NRKDFSNRVAGLEGSHLVRMKSASSTLADKVALVKEMQYHPITGDVIHADFYEVDLTA
                     RIEVSVPLHFVGKGAGVVRGGILQPIVREIQVECLPLDIPQFFDVDVSGLDIGDSVHI
                     EDLSMPEGVTAVYDSNFALVTVATPTVEEEPTAAAVATPEAAAVASPEEKKEEE"
     gene            12044..12610
                     /gene="pth"
                     /locus_tag="VF182_06605"
     CDS             12044..12610
                     /gene="pth"
                     /locus_tag="VF182_06605"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX6436781.1"
                     /translation="MKLIIGLGNPGRKYDRTRHNIGFRVVDDIAGQNSIAIKKKACES
                     LVGELIIMGDPVLLAKPQTYMNLSGRAVKALLKETRAAPENLVVIYDELDLPFGRIRV
                     RQKGSAAGHRGAMSIMESLAGAPFYRVRVGIGRPQGGLDPADFVLNPFSPEEVEQLDA
                     IVARAAEAALCIVQEGGQRAMEKFNRAN"
     gene            12676..13059
                     /gene="rpsF"
                     /locus_tag="VF182_06610"
     CDS             12676..13059
                     /gene="rpsF"
                     /locus_tag="VF182_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX6436782.1"
                     /translation="MTVTNYETLFIVHPEKGPRIKEFVERFKKVIEGQDGVVSQVEEW
                     GLRDLAYRIQKQGKGYYTLLQYRASGRAVEELERNLRLTDGILRYLTVRDDEASQAAA
                     QAPAGERTEQGDNPAPPDATTDSPA"
     gene            13367..13663
                     /gene="rpsR"
                     /locus_tag="VF182_06615"
     CDS             13367..13663
                     /gene="rpsR"
                     /locus_tag="VF182_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX6436783.1"
                     /translation="MAQRESGARANRDRMDDDKGGGSRRRPMFRRKVCRFCADKTLRI
                     DYKDVRTIASFVTERGKMTPSRITGNCARHQRLLTTAIKRARSVALLPFTTAKS"
     gene            13685..>14361
                     /locus_tag="VF182_06620"
     CDS             13685..>14361
                     /locus_tag="VF182_06620"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HEX6436784.1"
                     /translation="MQRLEIIARFFLATASTVMLFLGGVTVPPFAFALFPLVPQPVLS
                     FGIKYGGLRGMGVVILALAVLLFWGGEQLGYVYSIFVVLIGLLFLLLGRIRVLETLVL
                     GISAVVFTAFSLTLFLLYGSWQAIYTDVSQSLNSSLMSAVEMHEKMGFPQESIAVLKE
                     RAPEIATMILHLLPAAAFVCLGLIVLFNVLLLCRRFPEKRPHWVALVNLREWKAPEFL
                     VWGVIVSG"
BASE COUNT         3464 a         3520 c         3881 g         3496 t
ORIGIN      
        1 cgccgcatca ctggtcggcg gtatcggttt ccggcgattg ggattgcgca ggatttgaag
       61 tgatttctgt tcgatctgcc ggatacgctc gcgtgtgaca gcaaagatct cgcctaattc
      121 ctctaacgtg taatctcgct cttcctctat accgaagcgc attctcagga cggtttcttg
      181 tcggggcgtc aacaccgcca gagcttttcg cacgtcagtg cgcaggacgc cttccatcgc
      241 ttctttgtcg ggctgaggat tgatccggtc ctcgacaaaa tcggcaagtt gatcaccatc
      301 ttcccaaacg ggcgtctgca gcgatacagg ctctccctgt gtttgcatga ccctaagcat
      361 ttctggaaca gacatgtcca tctcttttgc cagctcttcc ggcttaggct cacgcccgag
      421 ggtccgttgg agatgatgag cggtctgaat aaccttattc ctgagttcga ctctgtggac
      481 cgggatgcga atcatgcgcc cggtgtcgat caaaccgcgc gtgatcccct gtcgaatcca
      541 ccagcttgca taagtagaga atcgatatcc taaacggtag tcaaatttct ccacggctct
      601 cattaaccca aggttaccct cctgaattaa atcgagtagg ctcaggccac gatttgtata
      661 cttcttcgca atgctgacaa ccagccgcag gtttgcctcc gtgaattcag tcttggctac
      721 attgacctga ctctcggtcg ccgttatctg ggagacgagg gcatgaatct ccgccgctcg
      781 aagcccgacg ccggattcca tttgttcaat ttgtgataca atcgccgcac gtttcgcctt
      841 gctcgcgacg tgcgcgcgtt cctcgagttc cgtgatggtg ttcgaggatt gttttagctt
      901 ctgcaccatg gattcgagac gggacgaaga aagcccaacc tcccggagca gcccgactat
      961 tctgttcaac aaactcgcgt gagctttgtt cagagcattg cgacgctgtt cggatacgcg
     1021 cttttgattc agctgtcggt gaaccttttc ttgctcgtgt cggagacgtt tgagtttgct
     1081 tatggctcta agaaaaggct tgggatcaaa gcttactcga ttctcgtctt cttctgcgtc
     1141 cgatttttcc aaaacttctc ggatatcgag ttcgccgcta gccacggcct cgcccaaacc
     1201 aatcgtgtag tgcaggcaca taggagcatt gaacagggcc tcgatgatcg cattcttgcc
     1261 aatgtccatt ttcctggcga gttcgacttc ccgttctcgg ctaagtagtg gaatcgagcc
     1321 aatttcatgt aagtaggttg taattggatc gaccgttagt cctttttctt caggctgaac
     1381 ctcgccttca ccagcatttt cttccgcggt agcgccatcc gactcgagtt ccaactcggc
     1441 ttcatccccc tctagctctt ctgtgtcatc tttttcgatg tccaacaatt catcattggg
     1501 tcgattggta tcaacatctg tatctgtccg catggtttct cctcaacata tgcgccttag
     1561 acgataacgc ccgtcgcttc gcagctgacg ggggtaaatg cgccagattt ctagctaaaa
     1621 cgcgaagcga attcggtgcc atttgccttc tcaattgttt tgccgtgatt ccctggagta
     1681 attctcatat gtgagacatt gggaccactc atggtccatg tcgacctgag aaacacgata
     1741 gctaaaggaa ctttggctcg agctattgga gtgtgcttgc ggcttactcg atcggttcgc
     1801 gggcatcatt aaatctaagg accgcggtgc tctatgtcaa gcgaaacacg tgtattcgga
     1861 cgttaattta agcatcgctg ggttgtaggg agcccgccgg attaaaccaa gatggtgggg
     1921 tcatattgat cgttctgtgt tggaagaccc tcttctgttc cgcgttcggt cgactgcaac
     1981 tctactaatc agagcagtag tgagccttga aggcgacaaa cctggcttcg gccatgagat
     2041 acttgagccg ggctgcaaac tgcaatcgaa atctcgcttg gacgtcacac gggcggtcaa
     2101 aagaaagggc gcacagaggt agagtcatcg gtgaaactcg aattggatta cgagcggcga
     2161 attaggtttc agacgaatgt gccgcttgca caagagatca tttcactgtg accttgtatt
     2221 ggaatggata cttgacaact cttttacgga tcggctagtg cttaccactg gccgccgagc
     2281 cgtggcatat tggccgcagt aagttgatct acttccaagg aggggcttcc gtgaaatcac
     2341 acgaatggcg acaagaattg ggtgaatgtg ttcgctacct ctttcgctct tctgaaatgg
     2401 aacacttcta ttcgattaag atgaccacgc aacgtgcatg tatctatcta ttgcaactaa
     2461 gcctatatgt gcggcaacgc cgtaacttct ggccccaagt ggctgcgaat tgccccgaac
     2521 tcgaggtgaa acagcgaatc atgtcccatg agtacgaaga gttagttgag gatgaacact
     2581 ccaagcatgg tcacctcgat ttgatttttc ggcaaggcaa agaggtgggg ctgagttcgg
     2641 ataagatcat aactgcgcag ccgctaccga ctacgacagc ggcgatttac gcctggtttt
     2701 ggattgcccg caatcggcca tggcaagagg caatcgcggc atcgaccatt gcggaatgga
     2761 ccaacgatga tcggctctta ggtgatcttg gcggcggcaa ctgcagccgg ttatatacaa
     2821 tttggaagac ggatttaaat cttagtgatg ggcaaatgcc caattttacg gctcacagca
     2881 aggctgatgt taaacattcg gatatgttta tagatgtact ggagaaatac gtcgcgccgg
     2941 gacgtgagca agatgtgttg aacaccgcta aagaatccat ggatatccat cgggcttact
     3001 ttggcggaat ggctcaagcc atgtcgaaag cgatgtaacc gcaggagacg cttatgaacc
     3061 ttgctgaatt agacagtaag ttagcgaaaa accaactagc cgggttctgg aacacccgtg
     3121 ttcctggtca ttctgcggaa gcgccctatc tgtggaaatg ggagtcgctg ctagatggct
     3181 tattaaaggc aagcgagacg attgggatcg agcaggccga gcggcgcgcc attcgactga
     3241 taagcccgca cgtaccgaac aaatcgacct ctcacacgat tcagttgacc ttttcgatcg
     3301 tcaatccggg agaggtggcg agagcgcacc gccataacat ggctgccatc cgcttcgttg
     3361 tccaaggcaa aggcgcttat accgcggttg gcggtgagaa gtttcctatg gaagagggtg
     3421 atttggttct gacacccaac tggacatggc atgagcatca taatgaatca ccagatccga
     3481 tcatctggct cgatggtttg gatggtccat tgatccaagc gcttaatgtc ctgttttttg
     3541 agcaatctga aaaaccggaa caaccgatca cacgcaccac cggtgaatcg ctgagtcgtt
     3601 taggttttgc ccgcgcgccg aaactggatg agactcaact gcgcggtgtt ccgttccgct
     3661 atgcctggca ggacacctac aagacgctca agctcatgag cagaaacgac caggatccat
     3721 acgatggcgc gttgctgcgt tacatcaatc cagccaccgg cgggtatacc tatccgacca
     3781 tgtcttgcga gattcagctt ttgaatccta agcaaagcac tcgtgtacat aggcatacca
     3841 gcacggcagt ttatcatgta ttcaaaggtc atggccggac cgccgtaggg gaaagttgcc
     3901 tggagtggaa taagggtgat tctttcgtga ttccgctttg gcagtggcat tcgcatgaga
     3961 atccattcga cgaagaagct attcttttct cgattaatga ccggccggtg atggaagcgc
     4021 tgcaacttta tcgggcggag atccgtaacg gcaacaacga tgctgaatga atcctcttca
     4081 ctggctctca cggttagcga acatccgtga cgcgcgtggc tagagaaatg gcggcaagaa
     4141 atcgtaacgt cgccgtatat cgtaccctat ggctgacaca aacataaagc tcgctagtcg
     4201 cacttacgat gggattctgc ccattttgcg cggccaaatg caaattcctg gctttcacct
     4261 tgagattgcc gaaaccagcg atgttgccgg catgtttgcc gggatgttcg cagggcgcta
     4321 tgacgtgggc gaaatgtcgt tgggcgaact gatctactac acttcgagaa ccagagccga
     4381 ttttgttggt attcccgttt ttccctccag aatgtttcgg cacggcttta ttttttgccg
     4441 ccgctcttcg gggatcgata aaccgggcga cttgagcggc aaaagcgtag gattcttgcg
     4501 ctgggtgcaa accgcagcaa tatggatgcg ggggatgctt gtcgatgaat atggcgtttc
     4561 ggcctccgct acgcgatggt atatcgcttc gatacatcac tgggatgatg ccgacccgag
     4621 tgctactgtc gagccgcgtg acggttcgat gattcaccag atcggcagta ccggaaaaag
     4681 tgcttccgaa cgcgcctgcc gggctctgtt caacggcgaa gttgacgctc tcggagtgac
     4741 tgaatcgcag cttccgagtt tgctggccaa tgataaggta ggccggctat tcgctgaccc
     4801 acgcgaggtg gaggccagct attttcgcaa gacgcgaatc ttgccgatca tgcatgttgt
     4861 cgcgatccga aaaaaattgg ttgagcaaca cccggaactg ccggggaaac tttttcgtct
     4921 ttactgcgag gccaaacggt gggcacagca atggcgccgc gcgatcccca gtttggtcga
     4981 ggcttggccg aatcattact tatctgaaga acaaaggata tttaacacag atccgtgggc
     5041 ttacggcttg gaggcgaatc gacacgtgct cgacactttt ctcgcctact gtgatgcgca
     5101 gggagtcagc gctaaaaaaa ttgcgcctga tgagttgttc gatcctagta ctttgaaact
     5161 tgtcgattga gtttcagaac ctgaatgaaa aggagtcttc aatgtctact gcactggcaa
     5221 gcaaaagtca gaaagttgcc gcctgcgatc tgcgcgattg gctccggcaa gtggacgcca
     5281 ctggacaatt ggcaactatt tccggggctc actgggattt ggaactcggc gcgctcaccg
     5341 agattatact cgaacggatg tctacgccac ccgcggtgat gttcgattct gttcctggtt
     5401 atgagccaaa tcgtcgcgtt cttgccaata tgctcgagac cttggaccgg accgcgctga
     5461 cactaaatct gccgctcgac ctcaagacga ttccattgat tgacgctctg cgcgccaaac
     5521 tgcgagcgct tcaaccagtc aagccaaatg tcgtctccac cggaccgata atggaaaatg
     5581 tcgaacgcgc cgaccagctt gatttgttca aatttcctgt gcctcgctgg cacgagggcg
     5641 acggggggcg ttatctggga acagcgcatc tagtaattac ccgtgatcct gagaccggcg
     5701 tagaaaacgt cgggtgttac cgagtcatgc ttcaggatag agacaaggtc ggtctttata
     5761 tctcgccggg taaacacgga agaatgcatt atgaaaaggc catgaaagca gggaagcctt
     5821 ttccggtcgc gatggtcttt ggtcaacatc cgctcctgtt catcgcatcg tctcaggctg
     5881 tacctttcgg tgttaacgag tatgactgga cagggggaat tctgggtcaa cctattgatg
     5941 ttatcaaagg accgctcacg ggactccatt tcccggcctc ggcggaaatt gcgattgaag
     6001 gcgagatcgt ccccggggaa atgctccccg aaggtccttt cggcgaatgg cctggatatt
     6061 acgccagcgc tcgccgcgcc gagccgtttg tgcgcgttaa ggcattgtat tatcgaaatg
     6121 atccgatcat ttgcggcgct gctcctttca agccgacgat tcacggtatg tatcgttcgt
     6181 gtttgcgcgc cgccaccgtt tggaacggga tggagcaagc cggcgtgccg gatattcgtg
     6241 gcgtttattt accgccgccg gctcagcgct tcatgatcgt tgtagcaatc aagcaacgtt
     6301 atcccggtca cgccaaacaa gctgctctga tggccgccca atgtcatgcg ggcgcctatt
     6361 taggtcgtta cgtcatcgtt gttgatgaag acatcgatat cacaaatatc aatgaagttg
     6421 tctgggcaat agctactcgc tcagacccag caacctcggt cgatattctt cgccgcacat
     6481 ggagtggccc actggatccg atcattcagc cgggccaaaa gggacataac tcgcgcatga
     6541 tcatcgaagc ggtgcggccg tatgagtggc gtgaccgttt tccagcgact agcgcgatca
     6601 gtgatgaaac ccgcaaagag tactcggcca aatgggcaaa agagcttggt gcaatcgagg
     6661 gtcgccaaac gcgaccacgt gcgcacctgg tttgatctcc gcgccgactg caagaaaccc
     6721 ttgagatcgc tcgccgcttg gcggtgcgga tcactgctca tgcttcctcg gtcgatttcg
     6781 agtagacgaa aattgtaata tttggccaat attgtccatg gccagagatt ctcagcgcct
     6841 cttggctgtg cctagcgttc cccgcctact aaaggaatta tttggaatta tcagtcgtta
     6901 cgcccgtgtg cctgctcctc gacttcgctg gcatgtcttg tgctccatgg cattactgaa
     6961 ctcgcgaaaa aattcggaag gagaattacg gatggaagag ataatgaccc cctctgaggt
     7021 tgcaaattta ctgaagatcc acctgaagac tgtttacaag ctagcggaga agggagttat
     7081 tcccggcaat cgtatcggga gaagttggag attcagccgg agcgacgtac tcgaattagt
     7141 ctcgagtaag cccatcaatc tgtcggacgg tgacaccgac gatcaggtta gtagactggc
     7201 tgctgtgaag taagcagaaa gcatccgagg tcattagcag tttgctctaa tcaatcagtt
     7261 tgctggcgct tttccagatg agattggtgc gctgttgttc aaagtcgcac ttggagcaag
     7321 ggttctaaga catgcggcag tcgacaagtc atgagaagac ttaaaacacg ggctaaagta
     7381 aaacatctcg agctcgagtt caggcggcat gaagaggcgt tgcttcactg cgttcgtcgt
     7441 atcgacgagc atcttctacg ctgtcgtaaa cgaattgatg attataaaga aaggttttcg
     7501 attctaatcg ggttgaacga gagacttgca aacttgggag aggatccggt tgaacttcga
     7561 tcgcatgtcg attcccagag tttcgatgac ttgatcttgt cgcgaatcga agctttgaaa
     7621 tcggaaggca agaccttgtc tagctagata gggcgagtgc aatggaaata ttcatcgctg
     7681 ttttcagtgg cgcggctttt ctggcgctga tcgtctatct ctggaacacc gatggtgctt
     7741 cgaaacaagg caaacaggaa ataatagaag aagaatccct atcggcggaa tcaaccacag
     7801 aaccaagccc acgttcatta attttgtccg ccaccgaatc cgaacaggtg gtggcgcttg
     7861 agcaaaaaat cgacgccgta aaccccgagc aggctgaatc attcaaggct cttgaggaga
     7921 aagctacggc ttacaaatcg gagctagacc aggcccaggc aagtgccgcc cataactcgc
     7981 aaagagttgt tgagttggaa gcagacaatt cgctattgca gcgccgattg actgaactgg
     8041 acgctcaaca gaggcatcga cagagtgaac tggcatccct cgatgaccaa gtggcgaaac
     8101 tcggcgcgat cgaacaacaa gttgaagttc ttcaaaaaga aaaagctgag ctgacgcaac
     8161 atctcgaaca gttgcaaatc gagaacgctg ccaatgctcg agcaactctc gccctacaag
     8221 atttggaagc taagcaatcc gagtatgaag aaaaactcgc gagcctgggc gcggaaaatc
     8281 gtcggctcct gggcgaaata acgggactgc agtcgtcact tcgagacaag atcaaaactc
     8341 agctggatgg acttcaggaa ctctatagaa atctgacccc ggggctgaac tgactccgca
     8401 ttagcggact cttcacgatc agggcttgaa tttatacctc gtggccgcga tgatccggcc
     8461 gtctacgcaa tcgcacctaa gcagtaacct tatctcgatt agcacaccgt gctggtgcgc
     8521 ccgacaggaa tcgaacctgt acttagggtt ccggagaccc tcgtgatgtc cttttcacta
     8581 cgggcgcctt caattctctt acatctgcac ctcttcagag tcaatgtgaa gaagcgctgc
     8641 cggaatgcgt ctcaaataaa atacactcct ctcgccggta gcggttcaac ataatgctcc
     8701 cgaccctaga atctcccggc ttctccttgc cgattcctgc cgcacggctc accattgatt
     8761 gcgatgcgaa agcatgtacg cattgaaatc tctgcggtcg catcgcgtaa tcaagtgaaa
     8821 tagcatttgg caaacgaaag tctcccatgc taccggtaag cgtgaagatt cgagctccgg
     8881 ccaaaatcaa cctgggatta cgcgtcgtag gcaaaagagc agatggatat catctgctgg
     8941 acacgattat ggttccggtt tccttgtatg acgagataca cctaacaaaa atgggcgagg
     9001 gacgcggcag cagttcgacg ttgactgtca cctgcgataa tccgacgctg ccgacggatg
     9061 aaggaaacat cgtcttccgt gccgcgcatc tcattataaa agaaaaattg attcgagagc
     9121 cggtggcaat tcatataaaa aagcgaattc ccatcggtgc cggattgggc ggcggcagtt
     9181 ccgatgccgc ggcaacgctc atggcaatga attctctttt tcggctcaac tactcgccgc
     9241 ggcagcttaa gaaacgggcc tataggctgg gcgctgatgt gccgttcttt atcgatcgta
     9301 ggcctgctcg ggccagaggt atcggcgaac gtctcacgcc gatcaagtct ctcgcgtgga
     9361 aatgggcagt catcgtttat ccgggatttc ctgtagcaac cagttgggtg taccggaatc
     9421 tcgagcctaa gttgacaaaa gctatggcaa atactagcat tatttcaccg ctcaggaagt
     9481 catttgatca acaaagattg cttgttaatg acttggagag tgtcactgtg gcgcgatatc
     9541 cccggatcag attcttaaaa gaaaaattga ttgcggaggg tgcagccgga gctctaatgt
     9601 cgggcagtgg gtctgccgtt ttcggcctat ttgattccag aggaaatgct gcacgggctt
     9661 tgtcccgatt gcagaaggag gacggggtag aaacgttttt agtgcgtttg ttaacttaag
     9721 atcgacatag gcaagcccaa attagagcac aagtcaggaa gtcggttaac ctgtgacaag
     9781 aggaggtctc gatgcaggtt acggaagtaa gagtctttcc tgtcgacgaa gataagctta
     9841 gggcttacgt aactataact ctcgatcatt gttttgtgat cagagattta aaaattatca
     9901 aaggaaccac cggatacttt gtatcgatgc ccagtaaaaa acgaaaagat ggtacctaca
     9961 aagatattgc gcatcccatc aataatgaaa cccgtaggat gctagaggag aaagtgatcg
    10021 ccgaatatga gagagttgct gcggagggtg ggagccagcg tgctctagga aacggctaat
    10081 taatcctctg cccagggccg aatgagcttc tatcgagaag cttggagatt gggcggtcgc
    10141 caagcggtaa ggccccggac tttggatccg gtatcgaagg ttcgaatcct tcccgcccag
    10201 ccagtgttaa tcttgtaatc agcgacagat tgtcatagtt tatgttgcgc cgttcttagc
    10261 cgaacggcgc aactcgtttt aacagtagac gattccattt aatcgtcgcg ccgatgtggc
    10321 gggtgctgta ttgccatcga tgttgcaggg ggagcatgta aacgcggaat gggaatccat
    10381 caccatgagc cagcagaaaa tcgacgtaaa gatctttgca ggaaactcta atcctccact
    10441 tgctcgggaa atcagtgatt accttgcagt gccgttagga aaggcgaccg tcgaaacctt
    10501 cagcgacggc gaaagcaagg tcgagattaa ggaaaatgtg cggggcgccg atgtttttgt
    10561 tttgcaatct acttcggcgc cgggaaacaa tcatttgatg gagctcttgt tgatgttgga
    10621 tgctttcaag agggcttcag ccaagcgcat caccgcggtg atcccttact acggttatgc
    10681 gcggcaagat cggaaagtgc tcccccgagt tccgatcagc gccaaactgg ttgctgactt
    10741 gattacaaca gcgggcgcat cacgaatgct gactttggat cttcattcgg gacagattca
    10801 aggttttttc gatattccgg tggacaattt gtatgcgacc ccggtgttac tgcagcattt
    10861 gaaaaagcaa ctaaaccaca acgaggtgac aattatttct cctgatgcgg gcggcgttga
    10921 gcgggctcgg gctatcgcga cccggctcga tgcatcgttg gcgattattg ataagcggcg
    10981 tgtcggcccc aacgtagtcg ccgagatgaa aattatcggc gaagtccagg atcaggtggc
    11041 gatcttagtc gatgacatgg tggatactgc cgggacgttg accatggcgg cagacgcttt
    11101 gaaaaaagaa ggtgcaaaga gaattattgg ttgttgcact catccggttc tttccgggcc
    11161 ggcgatccaa cggattgacg actcaccctt ggaagaatta atcgtaacca atactatccc
    11221 gttgcaatct gaagcgaccc attgcggtaa gatcaaggtt ctttccgtcg cgcatctcat
    11281 tggagaagct atccggcgca cacacgagga agaatccatc agttctttat ttgtgtaggc
    11341 ggatttaagg aggtcagcct tggaaacagt agagattgaa gttaacgctc gtgaaccggg
    11401 aagcaagcat caggctcggc gactccgccg ggaaggaaag attcctgctg ttctttatgg
    11461 cccgaaggtc caaccacttg ctctggagct aaatcggaag gacttttcga atcgagttgc
    11521 cggactggaa ggctcgcatt tggtccgaat gaaatcggct tccagcacac ttgctgataa
    11581 ggtcgctttg gtgaaggaaa tgcagtatca cccgattacg ggggatgtga ttcacgctga
    11641 cttctacgag gtcgatctca cagccagaat tgaagtgagt gtgccgcttc acttcgtcgg
    11701 caagggcgcc ggggttgtcc gagggggaat ccttcagcct atcgttcgcg agattcaagt
    11761 agagtgtttg ccgctggata ttcctcagtt cttcgatgta gacgtcagtg ggttggatat
    11821 cggagactcc gttcacatag aagatttgtc gatgccggaa ggggtcacgg ctgtttacga
    11881 ttctaatttt gcattggtaa cggttgcgac acccactgtg gaggaggagc cgaccgcggc
    11941 ggctgttgcg acaccggaag ccgcagcggt agcatctccg gaggaaaaaa aggaagagga
    12001 gtaatttgta tctgggaaat ttgcacccga ttagaatcga cgtgtgaagt tgataatagg
    12061 gttgggaaat ccgggtcgga agtacgatcg aactcggcat aatataggat ttcgcgttgt
    12121 cgatgatatt gctggacaga atagtatcgc gatcaagaaa aaagcttgtg agtccctagt
    12181 gggtgagttg atcatcatgg gcgacccggt attactcgcc aagcctcaga cgtacatgaa
    12241 tctaagtggc cgtgcggtca aagcgctgct caaagagact cgagccgcac ccgaaaatct
    12301 cgtggttatc tacgatgagt tggatttacc gtttggtcgc atccgcgttc ggcaaaaggg
    12361 tagtgccgct ggccatcggg gcgccatgtc gatcatggag agtctggcag gcgcgccatt
    12421 ttaccgcgtt cgagtaggaa taggacggcc tcagggcggt ctcgacccgg cggatttcgt
    12481 attgaatcca ttctcgccgg aggaggtcga gcaattggat gcaattgtcg cgcgggctgc
    12541 tgaagcagcc ttgtgtattg ttcaagaagg cgggcagcgc gcaatggaaa aatttaaccg
    12601 agccaattaa gcatcggctc tgcgttgtaa acgcaaatca accgaataaa aacgagcgtt
    12661 attttgaagg gaaatatgac tgtgacaaac tatgaaaccc tcttcattgt ccatccagag
    12721 aagggaccac ggatcaaaga attcgttgaa cgatttaaaa aagttatcga gggccaagat
    12781 ggagtcgtct cacaagtcga agaatgggga ttgcgagatc ttgcgtacag aattcagaaa
    12841 caggggaaag gctattatac cttgcttcag tatcgtgcat ccggacgggc cgtggaggaa
    12901 ttggagcgta atctgcgact cacagatggc atcttgcgtt atctaaccgt gcgcgatgac
    12961 gaagccagtc aagctgccgc tcaagctccg gcgggcgaga ggacggagca gggcgataac
    13021 ccggccccgc cggatgcaac aacggattct ccggcctaac gagtcggcgc cgcgattact
    13081 ttaaccgagt agacaagtca tcgtcccggc aactcgctcc ctatcccgaa cttcgcgaga
    13141 cggccggtct ctcgacgtag tcttcgatag gtacgatgcg gataatttct cacgttatga
    13201 gaatggatgc accgatttga cgagggaata gtaagcagaa gcatgattga ggagcaacac
    13261 ggagcctgtc ggggctagct acggcggtag ccagaatacg ccaccataga atcattgact
    13321 agaacgtcac gactgagaaa tttaaaggaa tgaggtagaa tagtttatgg cacagaggga
    13381 atctggcgcg cgagccaacc gcgatcgtat ggatgacgac aagggtggtg gttcacgtcg
    13441 ccgcccaatg tttcggcgga aggtatgtcg cttctgcgcg gacaaaacac tcagaatcga
    13501 ttataaggat gttcggacca ttgcctcatt tgtaaccgaa cgagggaaaa tgactcccag
    13561 ccgcataacg ggaaactgtg ctcgtcacca gagattgcta acaacggcga tcaagcgagc
    13621 gcgcagtgtg gcgctgttgc cgtttacgac tgccaaaagc taagggtaaa agattcttta
    13681 ttcgatgcag cggctagaga tcatcgccag gttctttcta gctacggctt ccacggtaat
    13741 gctttttcta ggcggcgtga ctgttccacc gtttgcgttc gcgctcttcc cattagtgcc
    13801 gcagccggtg ctttcgttcg gcattaaata tggcgggctg cgcgggatgg gtgtcgtaat
    13861 cctcgccctc gcagttctgt tgttctgggg tggagagcaa cttggctatg tttactcaat
    13921 ctttgttgtt ctgatcgggc ttttgttttt attactcggc cgcatccgag tactcgaaac
    13981 gttagtcttg ggaatatcgg cggtagtgtt caccgccttc agccttacgc tgtttcttct
    14041 atacggttcc tggcaagcga tctataccga cgtgagtcaa agtctgaata gtagcctcat
    14101 gtccgctgtc gagatgcatg agaaaatggg ttttccgcaa gaaagcatcg cggtactgaa
    14161 ggaacgcgcc cccgaaattg ccacgatgat tctccacctt cttccggcgg cggctttcgt
    14221 ctgtctcggt ctgatagtgt tattcaatgt ccttctgcta tgccgtcgat ttccggaaaa
    14281 aaggccgcat tgggtggcgc tcgtaaacct gcgagagtgg aaagcgcctg aatttctggt
    14341 ttggggtgta atcgtctcgg g
//
LOCUS       DASUVS010000118         5035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_913175, whole genome shotgun sequence.
ACCESSION   DASUVS010000118 DASUVS010000000
VERSION     DASUVS010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913175"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VF182_06625"
     CDS             <1..226
                     /locus_tag="VF182_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6436785.1"
                     /translation="WDDAIPYGKALIEAAPDRIVWGTDWPHPQWRKRMMNDAEEVELL
                     YRYVDGDPILLKKILVDNPARLHGFEEILS"
     gene            543..1604
                     /locus_tag="VF182_06630"
     CDS             543..1604
                     /locus_tag="VF182_06630"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HEX6436786.1"
                     /translation="MAETNPNYSTPSSVRSVTSYPARRGFKQGPVDPSLDYVIGVEDS
                     IDIHCHAHEGQQDALGVAKLASKSGMKGILYKTIVGRKDPAGTVGGVRTALESWCAEK
                     GFTPVHVWPGSSVTQGFLSKIEPAWSAKMIEAGVVGLWMPNNTSANTLSIVGGKPSAW
                     DKTADPKAHTEPLSWPEALKYGHYLLDDKGRLLPEIEEIFRMAHDRGAAIFFGHPTKP
                     EFKAMAEFSQKIGFKKGVVDHPFSPFVNLTIEEMKDAASAGLWLNFTFDELSPLLGID
                     PANMYKAIRTIGPEHCTLSSDAGEPLFPNSVESLRLLRGYMSAFGCNADEIYTMCTVN
                     PAFIVGLDLTTTQRASAAV"
     gene            1751..2728
                     /locus_tag="VF182_06635"
     CDS             1751..2728
                     /locus_tag="VF182_06635"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436787.1"
                     /translation="MIKSGIRVVLFCLLFLGFCAAAIAAQERAIRIGFPSVAFQELPL
                     FAASKRGFFRAEGLNVELVQIAGAPAVAALLSGDVDYITHNSRIVATAVRGGGVKSVF
                     NYVARPIYYLVTLPEIKQPKELQGRTVGVSSFGGAAYYITKQLLEYYGLNASKDVAIR
                     ALGQDSIRFSAMAGKAIQATLLPPDFAVRSKELGFNVLVYAGDIVDMPMGGLGTTDKR
                     LKEKRDEVRRVLRSLIRSLRFVNDDREGAKSLLIDTFKMTSTSAAEAYELGVKIFGRE
                     GAPSENGLKTLLAATQEELKHPTEIAPRTVMDLSILRDAQRDLAIGKTQ"
     gene            2918..3340
                     /locus_tag="VF182_06640"
     CDS             2918..3340
                     /locus_tag="VF182_06640"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6436788.1"
                     /translation="MEKPKRIVQPPDLPVRKSHSQVVAVTGGTLVFVAGMTSRSEKDA
                     APVHPGDMRAQLRQVCENIGRALRSVGGDYSAVVKTTTFTVDVDEYHRCRDERYKYFK
                     EDLPTSALIGVKRLAHPDLMVEIECMAVIPSERFKPAN"
     gene            3430..4218
                     /locus_tag="VF182_06645"
     CDS             3430..4218
                     /locus_tag="VF182_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX6436789.1"
                     /translation="MGETLIDTIKNMMRYSRSKMVLHCFFRLVTITTLLLGLGSLPKA
                     IAAQVKESSGTKIGIIGSGNIGSTVGTLWVKAGHQVLFSSRNPENLKPLVESLGSLAR
                     AGTVGDALAFSDAALIAVPYGAYPQIGKDYGQELAGKIVLDAGNAVPSRDGEISKEAG
                     KLGIGLTSAKYLPKARIVRAFNTLSYLVLANNAHRAGERMAIPIAGDDKEALIVASKL
                     IQDAGFDPVIVGSLQSAKLFARGGPLYGQQITAKEMQLRLKKLQ"
BASE COUNT         1202 a         1328 c         1384 g         1121 t
ORIGIN      
        1 ttgggacgac gcgattccct acggcaaggc attgatcgag gcggcgcccg accgcatcgt
       61 ctggggtacc gattggccgc atccgcagtg gcgcaagcgg atgatgaacg acgcggagga
      121 agtcgagttg ctctaccgct acgtcgatgg agatccgata ttgctaaaga agatcctcgt
      181 cgacaatccc gccaggctac atggattcga agaaatcttg tcttagtgac gatcacaatc
      241 aatcaataac ccgtgggaag tacggtttac cacaactcgg caattagatt gtttaatagc
      301 gtcacgcccg aattttttta tcgggggtcc aatcttcaac ccgtctgaat tcctgctaag
      361 ggcatgcggg aatgacgtat ctgggataaa caattttcaa cggaggaact tgcgcgaagc
      421 taccgacaga acggaacggc aatgcgtcga cgtggaagcg tccacatgcg gacgagaaaa
      481 cgtttgccgg acacattatg aagcgatatt gtcgactcaa tttgaaaacc tggagggctg
      541 caatggctga aacaaatcct aattactcca ccccgagctc agttcgctca gtaacctcgt
      601 atcccgcccg ccgcggcttc aagcaaggtc ccgtggatcc ttcgctcgat tacgtaatcg
      661 gcgtcgagga ttctatcgac attcactgtc acgcccacga aggccagcag gacgctctcg
      721 gagtcgccaa gctcgcctcg aaaagcggca tgaaaggaat tctgtacaaa acgatcgtgg
      781 gacggaagga tccggcgggc acggtcggcg gcgtccggac ggcgctggag tcatggtgcg
      841 ccgagaaggg ttttactccc gtgcacgtct ggcccggcag ctcggtcacg caaggatttc
      901 tttcaaagat agagccggca tggtctgcca agatgatcga ggcaggcgtc gttggcctgt
      961 ggatgccgaa caatacgtcg gccaacacgc tctccatcgt cggcggcaag cccagcgcat
     1021 gggacaagac tgccgatccg aaagctcata ccgaaccgct ttcctggccc gaggcgttga
     1081 aatacggcca ctaccttctc gatgacaagg gacggctctt gcccgagatc gaagagatct
     1141 tccgcatggc tcatgatcgc ggcgccgcaa tcttctttgg ccatccgacc aagccggagt
     1201 tcaaagccat ggccgagttc agtcaaaaga tcggtttcaa aaaaggcgtc gtcgaccacc
     1261 cctttagccc gttcgtcaat ctgacgatcg aggaaatgaa agatgcggcc tccgccggcc
     1321 tgtggctcaa cttcaccttc gatgaattgt caccgctcct cggcatcgac ccggcaaata
     1381 tgtacaaagc gattcggacc atcggtcccg agcactgcac gctgtcatcg gacgccggcg
     1441 agccgctgtt tccgaattcg gtcgaatcct tgcggctctt gcgcggctac atgtcggcat
     1501 tcggctgcaa cgcggacgag atctacacca tgtgcacggt gaacccggcg tttattgtcg
     1561 gactcgatct gacgacgacg caacgggcga gcgcggcagt gtaaagtcac cgaatccgcg
     1621 tgcgcgcaga tatacgtatc cggcgtcgcg ccaattttct cgtctctcga actcggaacc
     1681 tagaagaagg tcctaactgg gaacctagaa caaagtcgcc cgggatcgtt ccacttgcgc
     1741 gatacgatct atgataaaga gcggaatcag agtagtccta ttttgccttc tctttctcgg
     1801 gttctgcgcc gcagcgatcg ccgctcaaga gcgcgctatt aggatcggtt tcccatcggt
     1861 cgcttttcag gagcttccac tgttcgccgc gagcaaacgt ggtttttttc gtgcggaggg
     1921 acttaacgtg gaactcgttc aaatcgcagg agcgccagcc gtggcagcac tgctgtcagg
     1981 cgacgtggac tacatcaccc ataattcgcg catcgttgcc accgctgtcc gaggaggcgg
     2041 cgtcaaaagc gtctttaact acgtggccag gccgatctac tatcttgtga ctttgcccga
     2101 aatcaagcag cccaaagagc ttcaggggcg cacggtcggc gtttcgtctt ttggcggcgc
     2161 tgcctattac atcaccaagc agctactcga atactatggc cttaatgctt caaaggatgt
     2221 cgctatccgc gcccttgggc aagactccat acgattttcg gcgatggctg gaaaggctat
     2281 tcaagccacc cttctgccgc ccgatttcgc ggtccgatcc aaagagctgg gcttcaacgt
     2341 tttggtttac gccggagata tcgtcgatat gccgatggga ggattgggaa ccacagacaa
     2401 aagactaaag gaaaagcgcg acgaggtacg acgcgtactg cggtcgctga taagatcgct
     2461 ccgctttgtc aacgacgatc gcgaaggcgc caagtcgcta ttgatagata cattcaagat
     2521 gacgagcacc agcgcagctg aggcctatga gcttggggtt aaaatcttcg gtcgtgaggg
     2581 agcgccgagc gaaaatggtt tgaagactct cttggcggct acccaggagg aactaaaaca
     2641 tccgacggaa attgcgcccc gaacggtgat ggacctttct attctgaggg acgcacagcg
     2701 agacctcgcg attggcaaga cacagtagaa atggatcctt agatgtgtcc tgcgcttgca
     2761 agagatctta aaattcttgc agtgaagcaa acgagatgac gaactactcg acagttaatt
     2821 tagagatgag gaatcttcgc atcaaaacag ctgcgcctgt gttcgcaccg agtttgtggc
     2881 tcggctcgtc aaaactctaa attccaaagg agaagccatg gaaaagccaa agcgcattgt
     2941 gcaaccgccg gatctacccg ttcgcaagtc tcattcgcag gttgtggcgg taaccggcgg
     3001 gaccctcgtc tttgtcgcgg gcatgacttc gcgcagtgaa aaagacgccg cccctgttca
     3061 tcccggcgac atgcgcgccc aattgcgcca ggtgtgcgaa aacatcgggc gcgcgttgcg
     3121 ttctgtgggt ggcgattact ctgccgtggt caagaccacg acgttcaccg tagatgtgga
     3181 tgaataccac cgttgccgag acgagcgcta taagtatttc aaagaggatc tgcctacgag
     3241 cgcgctgatc ggagtaaaga ggctcgccca tccggatctg atggtggaaa tagagtgtat
     3301 ggctgtgatt ccttcagagc gctttaagcc cgctaattaa gaatttaaac tctcggtcat
     3361 gcagatgatc cttgcaccgc aaaacgggtt gggtaaaagg aattctgcac ctacgttgag
     3421 agagcacgga tgggagagac gttgatcgat acaatcaaaa acatgatgcg ctattcacgc
     3481 tcgaaaatgg ttctacattg tttttttcgg cttgtcacaa ttacgacact gctgctgggg
     3541 ctcggcagtt tacctaaagc tatcgcggct caggtgaaag aatcttccgg aacgaaaatc
     3601 ggaattattg gttcgggcaa tatcggcagc acggtaggaa cattgtgggt aaaggcaggc
     3661 catcaggttt tgttttcttc gcgcaaccct gaaaacctga agccactagt ggagagcctt
     3721 gggtccttgg cgcgcgccgg gaccgtaggt gatgctctgg ccttcagcga tgccgctttg
     3781 attgcggtac cctacggcgc ttacccgcag attggcaagg attatggcca ggagcttgcc
     3841 ggtaagatcg ttctcgatgc cggcaacgcg gtcccttccc gtgacggcga aatctcgaaa
     3901 gaagctggaa agcttggcat aggtctcacg tcggcaaaat atttgccgaa ggcgcgtatt
     3961 gtgcgcgcgt tcaatacgct cagttatcta gttctagcga ataacgcgca ccgtgccggc
     4021 gagcggatgg ccatccctat tgccggcgat gataaggaag ccctgatagt cgcatcgaaa
     4081 ctgattcaag acgcaggctt tgacccggtg atcgtcggct cgcttcagag cgcgaagctg
     4141 tttgcccgag gcggaccctt gtacggccaa caaatcacag cgaaggagat gcagttgcgt
     4201 ctgaagaaac ttcaataagc gcaacgacct caatccctcg gaccttaaac tcggcttacg
     4261 atttgctgct tcaaactcgg cttacgattt gctgcttcgc cgatcgcgga aagtatcgcg
     4321 ctcttcgtcc gtttgcccca agaagatacg atttgcgtgg cctacaatat gccatgatac
     4381 ggtgttcgga tggctggatc ggtactagct atctcacgta aggcatgaca ggcttgtctt
     4441 agccgcacac gatccattga cttccatctc ggtctcggga tccaaatgac ttatgttact
     4501 agcgtgctag gccacgatcg gctggacaga aggagatcga aaaatgcaac cgtataagca
     4561 acatccgatt tacgggatgg cagtttcaga accgggaaaa cgctggcgcc caaaagggct
     4621 ggtatttgct ccggacctga actgtacatt ggaagttaaa cgcctagaac cttcagactt
     4681 gattttcacg actaagaaag cagccgagga gcaagcgctc aggctatgca aagcgtggat
     4741 agacgcgaaa gagtgtgagt cacatagcgc ggctaaagat ttggcctctc cgaaggttaa
     4801 gttgtagcag ccattgctga acatttgcaa atatcccggc acaggctggc tagaacggca
     4861 ccggccgcag tctgaaaact tagacctgac agttcgtaga ggagagcagg ataaacctgc
     4921 cccctcgtgg aaatggggat tatttaatag gaagcgaaaa ccctttgttt tctttcttgg
     4981 ctcgtttcgc ggcccttttc tccatcaggg ttttgagtgg tttcttttta ttctg
//
LOCUS       DASUVS010000119         8285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_695875, whole genome shotgun sequence.
ACCESSION   DASUVS010000119 DASUVS010000000
VERSION     DASUVS010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8285
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695875"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VF182_06650"
     CDS             complement(<1..606)
                     /locus_tag="VF182_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6436790.1"
                     /translation="MGAPNEIQRPWPSYRKYPERLNLAQEVLDTSIERGFGNRPALIG
                     HGGAITYEELNRQVNALAAKLIELGLKERDTALIVMSNSAEFAVAFLATVKIGVIPVL
                     VNSLLSAHELRTIVEQAQTDWVFTEAARAAAVRELRRDGLFNRVVCAGESESAEISFA
                     SLTHDRGTTVRASDTGADEPAFIVYTSGTTGKPKGIVHAHRW"
     gene            complement(611..1525)
                     /locus_tag="VF182_06655"
     CDS             complement(611..1525)
                     /locus_tag="VF182_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6436791.1"
                     /translation="MDYKALKWSVHDNPSNPGEPAIGVITLNRPDRLNAVDPLMRLEL
                     DALCNEIARNSIVKVVIVTGEGRGFCAGGDLTSEAEVLGAFEGSMGITGPYKEMAEYF
                     FNDLRHRVLQSAMRKLEDLPQVTIAAINGPAVGVGLEMATLCDMRIASDRARFGEVAV
                     PAGFVPESGGARNLPKLVGIGRAMRLILTGEIIDAKEALRIGLVEEVVPHDKLLDTVF
                     DLAGKIAANPYLSVRQAKRLVKMYWNWNRSDEGYQQELDAVLEITRTKDCQEGMRAFS
                     EKRQPRYNYPYDAGWPFPSKTPAKKKQK"
     gene            complement(1989..3668)
                     /locus_tag="VF182_06660"
     CDS             complement(1989..3668)
                     /locus_tag="VF182_06660"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6436792.1"
                     /translation="MSNEKSATVTKLTMIPPRQYPDLHEHLDRLESEGLLIRIKEAVD
                     KDQEMHPLVRWQFRGGIKEKDRKAFLFEKPVDAKGKSYDIPVAIGILAANRTIYGIGL
                     GCDPTKVNQLWAEAKTNPIAPVEIPSEAAPVHEVIYQDEDLEVPGRGVDGIPVPISTP
                     GWDNAPYISAAHFITKDPDSGIHNIGTYRAMIKAPDRVGCNPSLELGQGIYVHWEKYR
                     ARQEKMPAALCIGGVPAISYVAAQKLNYDVDEFAVAGGLVKAPIRIVKAKTVPVMVPA
                     DAEIIIEGWVNTEWLEPEAPFGESHGHVNPKEYNPYMEVTAITRRKKAVLCSIISQVT
                     PSESSVIKKMAYEPLYLDHLKNTLGVKCVTRVMLHEPLTATQKLTVIQMRKPSRAEVW
                     RAMMGAVAFRPSMSKIVIAVDEDIDPENMDAVFWALGYRSQPHRDVQIIRGTDIGHAP
                     RHDARGSADDSCLLWDATLKETFPPISLPKQEYMERARKLWEKLDLPKLEPESPWHGY
                     SLGDWNPELDEEAKLAIAGEFWQTGKKIALERRRTEEIPLNTSYYGHQKEK"
     gene            4378..4725
                     /locus_tag="VF182_06665"
     CDS             4378..4725
                     /locus_tag="VF182_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436793.1"
                     /translation="MDWEKIKLISWGAVGGSLVTMYVGFNAAGWMTDGAARTMAKEIS
                     ATAVADRLGTICVAQFNRDAAKSQKLIDMKGKDNWDKSRYIDTQSWAIMPGEDKAESG
                     VSEACAKQLSTKS"
     gene            complement(4771..5682)
                     /locus_tag="VF182_06670"
     CDS             complement(4771..5682)
                     /locus_tag="VF182_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX6436794.1"
                     /translation="MRTAYNKLMSLIGLITPAFWEWFNHNAFQMAAALAYYTLVSLAP
                     LIIILIAIVGPFFGTAATENYIVGAIAGIAGEESARAVQAIVHNANQTGSGVLASAVG
                     FTLLLLGAGAVVGQLQQSLNVIFGVTPKPDVDWWAFIRARFFSYAMLLAIGFLLLVSL
                     VVTTVLAAISKYFGDLLPYFSAIWPAVDLVSSFTLVALLFAMIYKILPDVNIAWKDAL
                     IGAALTSFLFSVGKFLIGLYLGRSTVAAAYGAAASVVTILLWVYYSALIVFFGAEITQ
                     LYASQIGSGVNANELADKTEPAKSDPQ"
     gene            complement(5932..7128)
                     /locus_tag="VF182_06675"
     CDS             complement(5932..7128)
                     /locus_tag="VF182_06675"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6436795.1"
                     /translation="MRIFDSLRYRDYALFWSSDLLASLGHFVQEVALYWIAYEITDSA
                     MALGILGLCGAAPRLLLGALGGVLVDRYDRKRLLILIQFFSTIPVLIFLIVYTFGNLQ
                     FWHLLTVEILFGSIRAVNPSASQSILGELVPRQDLMNAISLYTIGFNFARIIGPSLGG
                     ILVLWIGVRGCYGVFLVSLIISGCGMALIRLKDQRAANSEQDFVREFKEGFQYVWASP
                     LILSSIVAAYTFSIFIVTYQRFMPVFAKEIFEVGPEGLGLLMAAPGIGAIASLTFLAS
                     VGERWNRAWLLWITTTVTPIFLILFCLSPVFWISVVLLGLVGAGQVSFRTISRVIIQM
                     EAPRDLLGRVMSVFNMDQGMRSLGSVVLGAFATVFGASLGLALTAGFSLIITTTLFYR
                     LLGRKA"
     gene            7389..7994
                     /locus_tag="VF182_06680"
     CDS             7389..7994
                     /locus_tag="VF182_06680"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HEX6436796.1"
                     /translation="MKHQSIRSERCRTCGVSATACLCDVVPRIDLRTKVCLVIHHREL
                     SRRSNTGLLAIRALVNSEVRVRGEQRERLDLEDLLTAQYRTFLFYPSADAIELNQALV
                     MQEPTPIQLLVPDGTWRQARKIHSRHPELRELPRVKIRAPSRAIFQLRAQSRPERMAT
                     LQAIAEALGVIEGDRVRVQLMKLYQAKIARTLKARGIATGQ"
     gene            complement(8010..>8285)
                     /locus_tag="VF182_06685"
     CDS             complement(8010..>8285)
                     /locus_tag="VF182_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436797.1"
                     /translation="IFHTDKEYTFRTLSKYLRIADRKIVESSYEAEMPALEQRLDIRE
                     AAIQPVLEDVAQSDPRARNIKPQQLIDRRYLDEMAKNGFLDKIWAGK"
BASE COUNT         1943 a         2245 c         2316 g         1781 t
ORIGIN      
        1 ccagcggtgc gcgtgaacga tgcctttggg cttgccggtg gttcccgacg tgtagacgat
       61 aaaggccggt tcgtccgcgc ctgtatcgct ggctcttacg gtggttccgc gatcgtgggt
      121 cagcgaggca aaagagatct cagcggattc cgactcgccg gcgcacacga cacgattgaa
      181 caatccatca cggcggagct cgcgcaccgc agcggcccgg gcggcttcgg tgaaaaccca
      241 atccgtttgc gcttgctcca cgatggttct gagttcatgc gcggagagta gcgaattcac
      301 cagaacagga atgactccaa tcttgaccgt agcgaggaac gccaccgcga actccgcgct
      361 gttgctcatc acaatcagcg cggtgtcgcg ttctttcaat ccgagttcga tcagctttgc
      421 tgcgagtgcg ttgacctgcc ggttcaactc ttcgtatgtg atcgcgccgc cgtggccgat
      481 gagagcgggc cgattgccga aaccgcgctc gatcgacgtg tcgagaactt cctgagccag
      541 attcaaccgc tccggatact ttctatagct gggccagggt ctttggattt cgttgggcgc
      601 gcccatcgac ttacttctgt ttctttttgg ccggcgtctt ggacggaaac ggccagccgg
      661 catcgtacgg atagttatag cgcggctggc gcttctcgct gaaggcgcgc atgccctcct
      721 gacaatcttt cgtacgggtg atttccaaca ccgcgtccaa ctcttgctgg tagccctcat
      781 ctgagcgatt ccagttccaa tacattttta ccaggcgctt ggcctggcgc acggagagat
      841 agggattcgc ggcgatttta ccggcgagat cgaatacggt gtcgagaagc ttgtcgtgag
      901 gtacgacctc ttcgaccaag ccgatgcgca atgcctcttt ggcatcgatg atttcaccgg
      961 tgagaatcag gcgcatcgcc cgcccgatgc cgacgagctt ggggagattt ctggcgccgc
     1021 cgctctcggg cacaaagccg gccggcacgg cgacttcacc gaagcgcgcg cgatcggacg
     1081 caattctcat atcgcacaag gtagccatct ccaagccaac gccgaccgcc ggtccattga
     1141 tcgcggcgat ggtcacttgc ggcagatctt cgagtttgcg catggcgctc tgcaagacgc
     1201 gatggcgcag gtcgttgaaa aaatattccg ccatttcttt atagggcccg gtaatcccca
     1261 tgctgccttc gaaggcgccc aacacttcgg cttcggaggt gagatcgccg ccggcgcaga
     1321 aaccgcggcc ttcgccggta acgatcacga ctttaacgat ggagttacgc gcgatctcgt
     1381 tgcacagcgc gtcgagttct aaacgcatga gcggatcgac cgcattgagc cggtccggac
     1441 gattgagcgt gatcacgcca atggccggtt cgccgggatt cgacggattg tcatgcaccg
     1501 accatttgag cgccttatag tccattgaaa actcctttgg atgatctaat taaaataggc
     1561 gcagaatagc accacgaagg acacgaagcg cacgaaggaa aatgcatggc agttaccaaa
     1621 cgagtttgtg tcttcacgca aaaattcacc ctgcacgctg cctcggatca atctgccatc
     1681 tattcttagt tcttcgtgct ctcccccgca aattgcggct aaggtgaaga gatgcgacct
     1741 ctacaagttc gtcacccccg aatgttctta tcgggggtcc agtccggatt tcgcctggat
     1801 tcccgctata agcctgcggg aatgacagag tcgtcagaga attagatccc gaaggacgta
     1861 ctctattttg attatgtgtt ggcgagtgta aactcatgac acactgcctt cagtagaaac
     1921 gaacgcgcga aaccatcagc ctctgagaac cggaagaccg aaaaagctag aggagcatca
     1981 gccagtggtt acttctcttt ttggtggccg taatatgaag tattcaaagg gatctcctcg
     2041 gtgcggcggc gctcaagggc gattttcttc ccggtctgcc aaaactcgcc ggcgatcgcg
     2101 agcttggctt cttcatcaag ctccgggttc caatcgccga gggagtagcc atgccagggt
     2161 gattcgggct ccagcttcgg caagtcgagc ttctcccaca atttgcgggc gcgctccatg
     2221 tattcttgtt tgggcagcga gatcggcgga aacgtctcct tcaacgttgc gtcccataac
     2281 aggcaggagt cgtcggcgga accgcgggcg tcgtggcgag gtgcatggcc aatgtcggtc
     2341 ccgcggataa tttgcacatc tcgatgcggt tgcgaacgat agccgagcgc ccagaaaacc
     2401 gcatccatat tttccgggtc gatgtcttcg tcgacggcaa tgacgatctt cgacatcgag
     2461 ggccgaaaag ccacggctcc catcatggcg cgccagactt ccgcccggct tggcttgcgc
     2521 atctggatca cggtcagctt ctgcgttgcc gtcaacggtt cgtgcagcat gacgcgggta
     2581 acgcatttga cgccgagcgt gttcttcaga tgatcgagat aaagcggttc gtaggccatc
     2641 tttttgatca cgctggattc cgacggcgtc acctggctga tgatcgagca taaaacggct
     2701 tttttgcgcc gcgtaatcgc ggtcacctcc atatacggat tgtattcttt gggattgacg
     2761 tgcccgtgcg attcgccgaa cggcgcttcg ggctccaacc attcggtatt cacccaacct
     2821 tcgatgatga tttccgcgtc tgccggaacc attacaggca ccgtcttggc tttgacaata
     2881 cggataggcg ctttgaccag gccgccggca accgcgaact cgtcgacatc gtaattcaat
     2941 ttctgtgcgg cgacgtagga gatcgccggc actccgccga tgcacaacgc cgccggcatt
     3001 ttctcctggc gcgcccgata cttctcccag tgcacgtaga tgccctggcc caattccaac
     3061 gacgggttgc agccgacgcg gtccggcgcc tttatcatgg cgcgataggt gccgatgtta
     3121 tgaatcccgc tatcaggatc tttcgtaatg aaatgcgcgg cgctgatata aggcgcgttg
     3181 tcccaacccg gggtcgaaat cggcaccggg atgccatcga cccctcgccc gggtacttcg
     3241 agatcttcat cttgatagat gacctcgtgg accggcgcgg cttccgaggg aatttccacc
     3301 ggcgcaatcg ggttggtttt cgcttcggcc cataactggt tgactttggt tggatcacag
     3361 ccgagaccga ttccgtagat tgtccggttg gcggcgagga tgccgatggc gacgggaata
     3421 tcgtagctct tgcctttggc atctaccggc ttctcgaaaa gaaatgcttt gcgatctttt
     3481 tccttgatgc cgccgcgaaa ctgccagcgc accagaggat gcatctcttg atccttgtcc
     3541 accgcttctt tgatccggat cagcagtcct tcgctttcga gcctgtcgag atgttcgtgt
     3601 agatcaggat actggcgcgg cggtatcatt gtcagcttcg ttacagtcgc agatttttcg
     3661 ttgctcatag gtcctgccat tcgtttttaa ttagcgccac cttatatcaa ttctcaaccg
     3721 ggcgtaaagt aggtgggcgc ggatgacgaa cttcgacatg gggtagtcgg cccgccagct
     3781 actttcatga cgtatatcac tgaagatcct gtggtcgggg tcgttgctta tggagccacg
     3841 atccaagata aaagcactag gagagcttat cgaatagtta tcggcaatct gctcaattag
     3901 cgactgccgg agagcttgcc tcaggatgac ggcgcttacc cccgtcccat aaataatgct
     3961 tcagatttgt tcaatagtag acagcaatcc cggtggagtc ggcacaggct aagaaccaaa
     4021 gtctgtgcta agtagttctg acatgaggtc tcgcatgaaa gaaatggaag gtatctatgg
     4081 cgttttatgc ggttgatgtc ggaaaccatg tcatcgaacg ggtgactcaa caatatgcag
     4141 tcggccgcaa gaaaagagtt tttcttatag aagctgtttc tgcaaaacag gcttgggcaa
     4201 aggctggacg cgtctcgggc gcgaatggca gcgccgattg tgagcgctgc cgccatcgcc
     4261 actgtaaagt ttgcgcagag tactccgtca caaacagata ctcggactat tggatttgtc
     4321 actgctgcag tgagttgaac cggcgtgctc aacgtccgca cctgagggag gtgtctgatg
     4381 gattgggaaa aaattaagtt aatatcctgg ggtgccgtcg gcggctcgct ggtgaccatg
     4441 tacgtcggct tcaacgccgc cgggtggatg accgacggcg cggcccgaac aatggcgaag
     4501 gagatttccg ccaccgcggt ggccgaccgg ctaggaacga tttgcgtggc gcagttcaac
     4561 cgggacgccg ccaagagcca gaagctgatc gacatgaagg gcaaagataa ctgggacaag
     4621 agtcgctata tcgataccca gagttgggcg atcatgccgg gcgaggacaa ggccgaaagc
     4681 ggggtctcgg aagcctgcgc caagcagctg tcgacgaaga gctagcaggc gctaaaaaac
     4741 tcagacccgc gttttccagg catctttcaa ttattgcggg tcgcttttag ccggttcggt
     4801 tttatcggct agttcgttgg cattgacgcc gctgccgatt tgactggcat atagctgggt
     4861 tatttcggcc ccgaaaaata ctatcagcgc cgagtaataa acccagagta ggatcgtcac
     4921 cactgacgcc gctgcaccat aagccgcggc caccgtgctt cttcccagat acaggccgat
     4981 taaaaatttc ccgacgctaa agagaaagga tgtcaaagcc gctccgatca gggcatcttt
     5041 ccaggcaata tttacgtcgg gcaaaatctt gtagatcatc gcaaacagga gcgcgaccag
     5101 cgtgaaagac gagaccaaat cgacggccgg ccagatcgcc gaaaagtagg gcagaagatc
     5161 gccgaaatat ttgctgattg ccgctaaaac agtcgtgaca acaagcgata ccagcagaag
     5221 gaaaccgatc gctagaagca tcgcgtaaga aaaaaatcgg gcgcggatga aagcccacca
     5281 gtctacgtcc ggcttcggcg tgactccgaa aatgacgtta agcgattgct gcagttgacc
     5341 gaccacggcg ccggcgccga gcagtaaaag ggtaaatcct accgcggacg cgagcacgcc
     5401 gctgccggtc tggttagcat tgtgcacaat tgcctggact gcccttgcac tttcttcgcc
     5461 cgcaatcccg gcgatcgcgc caacgatgta gttctcggtt gctgcggtcc caaagaatgg
     5521 gccgacgatg gcgatcagaa taatgatcag cggcgccagt gaaacaagag tgtagtaggc
     5581 aagcgcggcc gccatctgga aggcgttgtg attgaaccac tcccaaaagg cgggggtgat
     5641 caacccgatt agcgacatca gtttgttata ggcggtgcgc acggcggcat gttgttgcgg
     5701 ctatagccat agaaagataa atacctgtca tctacaacgg tgaaaggggg ccacggcatg
     5761 caaaggacag aagaagtccg agtaagattg agtccttcaa atgagtccgt agtgatcaac
     5821 tcttaccgct atgactttat accaatcaat ccaaagctgc gcgaggatgg cagcggtaat
     5881 gattgcttgc gaaattcaaa cctccactga gctcctggcg agagtttccg atcacgcttt
     5941 ccttcccagc aatcgataaa aaagtgtcgt ggtgatgatc aatgagaagc ctgccgtgag
     6001 ggcgagccca agagaagcgc cgaacacggt agcgaacgcg ccgagaacta cggaaccgag
     6061 cgagcgcatg ccctggtcca tattaaaaac gctcatcacc cgtccgagca gatcccgcgg
     6121 cgcttccatc tgaataatca cccggctaat ggtgcggaag ctgacctgtc ctgctccgac
     6181 gaggccaagc aggaccaccg aaatccaaaa caccggcgag aggcaaaaaa gaatcagaaa
     6241 aataggcgtc acagtcgtcg taatccacaa aagccatgct cggttccaac gctcgcccac
     6301 tgacgcgaga aaagtcagcg acgcgatggc gccgattcca ggcgctgcca tcagcaaacc
     6361 caacccctcg ggcccgacct caaaaatttc cttggcgaag accggcataa aacgctgata
     6421 ggtgacgatg aaaatgctga acgtgtaggc tgcgacgatg ctgctcaaga tcaacggcga
     6481 agcccaaacg tactgaaagc cttctttgaa ttcgcgcacg aagtcttgct cggaattagc
     6541 ggcgcgttga tctttcaagc ggatcagcgc cattccgcat ccggaaataa tcaggcttac
     6601 caaaaagact ccatagcagc cccgcactcc gatccacagg accagaatgc ctcccaacga
     6661 cgggccgatg atgcgcgcga aattaaaacc gatggtatag agggagatcg cattcatcag
     6721 atcctggcgc ggcaccaact cgccaaggat agattgggag gcggatggat ttacggcgcg
     6781 aatcgagccg aaaagaattt ccaccgtgag caagtgccag aattggagat ttccgaaggt
     6841 atagacgatc aggaagatca acaccggaat ggttgagaag aattggatca gaatcaacag
     6901 gcgcttgcgg tcgtaacggt ccaccagcac gcctccaagc gcgcccagca gcaaccgtgg
     6961 cgcggcgccg cacaggccca ggatgcccaa ggccatggcc gagtcggtga tctcgtaagc
     7021 gatccaatag agcgccactt cctggacgaa atggccgagg gaagccaaca ggtcactgga
     7081 ccaaaatagc gcatagtctc tgtagcgcag cgagtcgaag atgcgaatgg gttgctctcc
     7141 ttagcaaagc aatctttata gcgtgtccgc cgcctataag aaaggaaatg gttgcaatga
     7201 aagtctcgaa gcgaagggat tcgatctccg aacttgggct ctaagcacgc cagttaccct
     7261 ctcgtgcgga atatgacatg acgtgttaaa cagaagggcc acgatgcgta taagcagagc
     7321 cggttgcgag gctacgtaca actagcgatg atcttacgaa aaaagccagg cggttcttgc
     7381 gctcgcagat gaagcatcag tcaatcagaa gcgaacgctg ccggacctgt ggagtctccg
     7441 ccacggcgtg cctatgcgat gtggtaccta gaattgattt gaggaccaag gtttgtctgg
     7501 taatccatca cagagaactg tcgcggcgca gtaataccgg tctcctggcg attagagcct
     7561 tggtcaatag cgaagtgcgc gtaagaggtg agcaacggga gcgtctcgat ctggaagacc
     7621 tgctgacggc acagtatcga acgtttttat tttatccgtc cgctgatgcc atcgaactca
     7681 atcaagcgtt agtgatgcag gagccaacgc cgattcagct cctggttccg gatggaacct
     7741 ggcggcaggc gcggaaaatt cattcacgcc acccagagct aagagaattg ccaagagtaa
     7801 agatcagggc gcccagtcgt gcgatttttc aactgcgcgc ccagagcaga ccagaacgga
     7861 tggcgacgtt gcaggcgatc gccgaggccc tgggagtcat agaaggggat cgcgtgcgcg
     7921 ttcaattgat gaagctatat caggcaaaga tcgcgcgcac actgaaggca cggggaattg
     7981 caacgggaca atagcagtgg cgcggtgatt tatttgcctg cccatatctt atctagaaat
     8041 ccatttttcg ccatctcatc gagatagcgc cggtcgatca gctgctgtgg tttgatattc
     8101 ctggctcgcg gatccgattg tgcgacatct tcgaggactg gctgaatcgc cgcttctcga
     8161 atatcaagcc gttgttccag agcgggcatc tcggcttcgt aggaactctc gacaattttt
     8221 cgatcagcga tgcgcaggta cttcgataaa gttctgaacg tatactcttt atccgtatga
     8281 aagat
//
LOCUS       DASUVS010000120        25628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_478199, whole genome shotgun sequence.
ACCESSION   DASUVS010000120 DASUVS010000000
VERSION     DASUVS010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 25628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25628
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478199"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VF182_06690"
     CDS             complement(<1..593)
                     /locus_tag="VF182_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436798.1"
                     /translation="MPKSKSQEKEIRSGLEALRQAGIAVDDPSPSLVQRLQGEIGKDR
                     QVDLAIVFTLGRIAEAAAVDALAQIGTEADDKMLKKEIRRSLFKLSQKGLAVPEARTS
                     GSATVLSLLKSASDIEAYMSSVDGAGGRLLWIVKPQAGHGLQTIQAMVSDREGLLRIG
                     GAQIRRKELRNMTREIKDKHGINMISLPWEYADQTLYA"
     gene            complement(610..1554)
                     /locus_tag="VF182_06695"
     CDS             complement(610..1554)
                     /locus_tag="VF182_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6436799.1"
                     /translation="MKVRPKRAPIKWPDNARIAITPCVAFETWPEDLGGPRTLQQENR
                     RSFPANAVTKRNLAIITDREFGERVGIYRMLEVFEREKIHTTFFPTGITVQNFPEIFK
                     EIAAQGHEVGTETWIHDYSYMKKRDKEKSDLKKTVDAVSKVVGKPPVGYLSTGVAPSG
                     ATPEIILELGYSYWMDPQHEETPYTLKVGRKELTVLSYFQDLNDYSSYQRAGRTPRDL
                     LQMWKDCFDCLYEEGSSDPKFMMWGNHPFVGGRPFRALLLREFIRYAKSHKDVWFATA
                     GQIAQWYRENYRDAQVETWPNFAFAGRQGKISDLMSAK"
     gene            1780..2571
                     /locus_tag="VF182_06700"
     CDS             1780..2571
                     /locus_tag="VF182_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6436800.1"
                     /translation="MAEGKMIESRVDNGIAVLEINRPPANAYTTELLKELDAAILDAR
                     FDDNVHAIVITGKLERFFSAGADIKMLGSASLSVKNNFALHGHEVLMRLENTPKVVIA
                     AINGHAQGGGLEIAMACDIRIAKKEGGRIGLPEINLGVIPGMGGTQRLPRLVGKARAM
                     ELCATGKLISFDEGLEMGLIHYIYEKEHFMEEVMDYARQFIVPNKSSLAVGKAKRAIT
                     TGSELSLPDGLAFERELLAQTFASEDGNEGVKAFFEKRTAQFKGK"
     gene            2879..4342
                     /locus_tag="VF182_06705"
     CDS             2879..4342
                     /locus_tag="VF182_06705"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6436801.1"
                     /translation="MAKMFIGGEPVDSVTKETYEIRNPATGAVVDSIPKGSEKDVQQA
                     IQAAESAFKEWADVTPEDRGNSLMNACELIKKNTSEIAQLLTQEQGKTLFEAGLEIHH
                     LVHGLEFYGGLASKVRGAHVPLPQKGAYGMVIRQPIGVCGGIVPWNFPLTLMGTKLGP
                     ALAAGNSIIIKPASTTPLATARVIELIAQATFGEGKAQKTLPKGTVNVVTGPGSSVGE
                     EILRNPRIRRIAFTGSTPVGRHVMEVAGREIKRVTLELGGSDPMIVMEDANIDLAIKM
                     ADVGRYFNCGQMCLGVKRLFLQESIADKFVGGLVEILKKKTVGNGMSKESRIGPIHLE
                     SQRKEVEEQVEDAKSRGAKTLFGGERPKGGDFDKGFYYTPTLMSNVPEDARMTTEEVF
                     GPALPVYTFKTLDEALEKANSSEFGLGSSIWTRNLLSANKAIDKLQAGNVWVNSLHYG
                     YDELPFGGVKSSGLGREHGPEALDYYLEPKGVVIVNV"
     gene            4517..5197
                     /locus_tag="VF182_06710"
     CDS             4517..5197
                     /locus_tag="VF182_06710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6436802.1"
                     /translation="MTSEKDEEYFACLKVSPAQMDFLWAEGWRHFGIYFFRYHTSFHG
                     DKKFSVLPLRIDLGRFTLTRSQKRVLAKNRDARIVVRPAAVDPGRHELFDKHRVRFKD
                     NVPTSLEDILSPMPASVPCPSRELCVYLGERLVGATYLDLGHDATSAVYAIFDPAEAK
                     RSLGILMILQSIEFSRERRYRYYYPGYAYREPFAYDYKKRFSGLQYLDWDAGWRAYEH
                     GSNPGDAL"
     gene            5346..6149
                     /locus_tag="VF182_06715"
     CDS             5346..6149
                     /locus_tag="VF182_06715"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6436803.1"
                     /translation="MFAQMKHMAIVSSNCNQEGDFYRDVFGLKRSGLARAGGAVVVRD
                     GYVGLNLNPRAPGRQAGFDHFGFEVEDVETVFSRVNEKYPKIKILKRPGNRPFAGISL
                     HDPAGNVFDLSQKGMENRRDLYVDDDQTMPRHVSHIALRVLDADGIAEFYRTVFELME
                     LEKPAGDPNHYLSDGRVTLAIFPWQITDYEGSGIERPALDHIGFKVESMPAFKADFDK
                     YMAANSPVIADRPEKNPEREVRRNLLSKCCFGQFEMSDPDGVLIDVSEA"
     gene            complement(6158..6979)
                     /locus_tag="VF182_06720"
     CDS             complement(6158..6979)
                     /locus_tag="VF182_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436804.1"
                     /translation="MASGWLNLRLEAPQPIFFSTDFPFAEGSILTELRLPLKAGKVEW
                     KYNFPIRGEYRLLVEVLAPDGTQSSKTFKIDIREHRSKWLLLAAFILGLFGFGVLAGR
                     IFTHALSRRGAGALPIIFSASLFFALPSPTALARAAPAWPITRLEIDPPSADKLAQIR
                     WILESDGGNASRGALLSVAITHIEKKITVFAVDRIPVEGEFSMKFLFVDGAEHRVSAQ
                     AQLPDGRSVRSEQVLAVDGVQPPESAMLPAMGLFLVVIALGLGVGRWSRYHATQS"
     gene            complement(7150..7338)
                     /locus_tag="VF182_06725"
     CDS             complement(7150..7338)
                     /locus_tag="VF182_06725"
                     /inference="COORDINATES: protein motif:HMM:NF021038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtB domain-containing protein"
                     /protein_id="HEX6436805.1"
                     /translation="MEKTFVEWLGVTVSEPVSFMMKSGMLLILSGTILWIALAAHYPA
                     VHDAMHNVRHALAVVPCH"
     gene            7505..8521
                     /locus_tag="VF182_06730"
     CDS             7505..8521
                     /locus_tag="VF182_06730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6436806.1"
                     /translation="MQFGVFDHLDRNDLPLREYYEGRLKLAEAYDHAGFYAYHVAEHH
                     STPLGMAPSPSVFLSAVAQRTHRLRFGPLVYALPLYHPMRLIEEICMLDQMSGGRLDI
                     GFGRGASQIESAMYGNDPAKAELMYVEGLELIMRGLTQKSLNFHGQFYSFDDVPMELE
                     PLQKPHPPVWYGVHSPVSAERAARQGLNIVSLDPADKTRTFTDRFRQVWCESENRQTR
                     EPMVGLGRFIVVAAKDEEALDIARRAYPRWHRSFNYLFNLRGGKGPVHQRPADVETLI
                     KIGQGIAGSPNTVSEFLKAQMTESGANYLVGQFAFGDLSLSEILSSLELFTRHVMPEL
                     RGID"
     gene            complement(8556..9224)
                     /locus_tag="VF182_06735"
     CDS             complement(8556..9224)
                     /locus_tag="VF182_06735"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018294875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6436807.1"
                     /translation="MGQMIAFKRPDGAECNGYLASQTGNDNAPGLVIIQEWWGLNDQI
                     KAVADRCAAQGYRALVPDLYKGKVTLDAAEAKHLMTTLNFADAAGQDIRSAAQHLKQS
                     SKKVGVVGFCMGGALTVLAAVFVPEADAASSWYGFPPEGAADVTKINIPLQLHLAEKD
                     ASFTPEAARALEAKLKGANVPCESYWYDAGHAFGNEKGQNYNKQAADLAWQRTTQFFE
                     KNLK"
     gene            9240..9854
                     /locus_tag="VF182_06740"
     CDS             9240..9854
                     /locus_tag="VF182_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436808.1"
                     /translation="MAVNFLVTIKAIPEVNVVRSITRKSEVLNSGHMKIKRSLRLHGV
                     AILALGMLTITLPQSWDLTAAQEVTDEIRQEQTKARQQQRLEELRRQEQIRQLQRDQK
                     INEQQQQLDRIQRQAPRSDTLTKQDASGVQQQLDQQQIEQQLQRQQNEMQIDRIQREQ
                     DQLRQQQRIDQLQREQQIDNLRNQLRRNQVQQDLNRLRQQQIRR"
     gene            10154..10984
                     /locus_tag="VF182_06745"
     CDS             10154..10984
                     /locus_tag="VF182_06745"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6436809.1"
                     /translation="METVGFVGTGAMGTALLSRLQLVPVSSKAFDVLPAALEQAKALG
                     AEPATSAKAVAQRSTLIDVVVRTDQEVLDCTLGDEGLLEGAAPGTLVLLHSTILPTTT
                     KLVAQAAADKRVYVIDACMTGVPRVARDGGLTFLVGGQKALFNRAKPHLLKMGKDAVY
                     MGSLGCGNVTKLIKNLVTASEALIVYEAIQIGKAAGIDYRAALDLMKKTQSEPFLSRW
                     DTRFDVSGSELKFNSGANLYDKDLPLAAELGNALGVEIPITEQLAELGTRLSGLDLAV
                     "
     gene            11050..11289
                     /locus_tag="VF182_06750"
     CDS             11050..11289
                     /locus_tag="VF182_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436810.1"
                     /translation="MRIRNYRDHKIELLSELTNSGEWVAQAMIVIIVAGKAKRIPIFG
                     RRRATFDSKRGADAYALELAKLWVDGRLWGGNGRG"
     gene            11379..11984
                     /locus_tag="VF182_06755"
     CDS             11379..11984
                     /locus_tag="VF182_06755"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6436811.1"
                     /translation="MHDVSCCLILMMLLVLLLTSCAIGPSTKGSSLHNPAQRVFLVRH
                     AERFNDSREDPPLTAEGKERAKALADMLRDAGVSAIITSQWIRAKDTAQPLADLLKIT
                     PEVIPTVDPPHEYFQATANAVRRIRNDTVLVVGHITIPNIIAELDGPQLPTICESVFS
                     DLFLLVPALGWDGLTRLRYGAAENISSGCRNLPEFLRPDSN"
     gene            12303..13091
                     /locus_tag="VF182_06760"
     CDS             12303..13091
                     /locus_tag="VF182_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6436812.1"
                     /translation="MAGPSYGRELAVRMLPPILAITSVLAVWWGLYLIFPRLLPGPLS
                     TFDEAMRLLSDGTFFFHMLQSLRRVFVGAAIAMIFSIAIGIYMGTVLMGERFFQPLVV
                     LGLTIPGLMWALIAVMLFGINEIASYFAVAVTTFPMLVINIWAGVKSLDKDLIDMSHV
                     FRFTPWMKISQVIIPQLVPNIFAATRYGLGLAWKVVVVVEMFGTSNGVGYQVMKSYQV
                     FNMEGVIAWTLTFVAAMIIIEYGIINLAERRLTAWRPKVDVWRR"
     gene            13092..13856
                     /locus_tag="VF182_06765"
     CDS             13092..13856
                     /locus_tag="VF182_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6436813.1"
                     /translation="MARLIVRDVEKWFRREDGRPLKILDRLSFEALEGCVTVVVGPSG
                     CGKSTLLNAIVGLERLDRGELAFAKDDVHLQRRPLIGYVFQSPRLLPWKTVADNLRLA
                     LQGAGIARGEWQERVERYLDLVALREYADQFPLYLSGGQRQRVGLARALAIESDLVLM
                     DEPFASVDELTARQLRQTTRRLCEQLHRTVLFVTHNLPEAAYMSDYVVTLTTRPARLA
                     EYVRNPLPAPRNWTPEIYRFAAELEAKVTFKEESEL"
     gene            13853..14815
                     /locus_tag="VF182_06770"
     CDS             13853..14815
                     /locus_tag="VF182_06770"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX6436814.1"
                     /translation="MNLSIIISAIAIIVEVGSFAMAQAQSLQTVNIATAAAGQSGYVA
                     ALIDAKGIAAKHGLKINNLMMDFTEAANAVKLGRAIASAMQPSTAVNLLKAGTDIRLI
                     TPQIWSGNSWLVKKDSPYKSLADLKGKKIGNFARVTGAYFFSAVIAKEKKLDIEKDFQ
                     SIPAETGALIALLERGEVEAINMFEPHTTRLVLSDKYRVLLDFDMELEKLFGAPPLKS
                     SVGMLRETVEKQPGLVKAIRGAYVEAVKVIKAGQDKDFFESKAKEFFNLSTPEQVAAG
                     LKQNRQNFADIWGESFFQSQNKILQSGISLGLLPKVNLNDLWVK"
     gene            complement(14871..15527)
                     /locus_tag="VF182_06775"
     CDS             complement(14871..15527)
                     /locus_tag="VF182_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436815.1"
                     /translation="MKTASTLVAPDLTFNGEAPWQTEGFDQTPASSYARDLRVVGQLL
                     ERRQITNADLVCVADAYIIRGIQRLTGDSRFSGPFSRRLMTRLDAMLHGDFRRSLGSG
                     IVDVRYTLEEIVKFDYLARCKRSKISPLPDPYSLSHILRSSGAYLDGRKNSSLIGISL
                     QEQWITIRFETAEGYPEEAKQNLQYFYDYWVKMYLRRGSQGQTSGTNLQRNSFVSNRS
                     "
     gene            complement(15921..17405)
                     /locus_tag="VF182_06780"
     CDS             complement(15921..17405)
                     /locus_tag="VF182_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX6436816.1"
                     /translation="MGKPTGFLDYERELPGDRSPLERVNDWREFHEHLSQAKLQEQGA
                     RCMDCGIPFCHTGQLISGMASGCPVNNLIPEWNDLVYRDLWQEALHRLHKTNNFPEFT
                     GRVCPAPCEGSCVLGLNELPVTIKNIECSIIDNGFAQGWVKPELPENRTGKKVAIIGS
                     GPSGLACAAQLNYVGHWVTVYERADRVGGLLMYGIPNMKLDKAIVQRRVNLLSAEGVK
                     FITNTAVGRDIRAEKLKDEFDAIVLCCGATKARDLPIPGRELDGIHFAMDFLHSNTKS
                     LLDSNHKDGRYISAKDKDVIVVGGGDTGTDCVGTSMRHGCKSLTQLEILPRPPDTRQP
                     DNPWPEWPKVYKLDYGQEEAKARFGDDPRIYLTTGEKFVGDANGRVKELHVYDVEWVK
                     NEQGALIPKRVPGTEKKLRADLILLAMGFVGPEETVLAQLGVERDPRSNAKAEYGEFS
                     TNVPGVFAAGDCRRGQSLVVWAINEGRGAARECDRFLMETTKLT"
     gene            complement(17632..22230)
                     /gene="gltB"
                     /locus_tag="VF182_06785"
     CDS             complement(17632..22230)
                     /gene="gltB"
                     /locus_tag="VF182_06785"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006630922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX6436817.1"
                     /translation="MTLSGLPPKQGLYDPTFEHDACGVGFVVHIKGEKSHEVVEQALT
                     VLENLDHRGACGCEENTGDGAGILLQVPHEFLERTCEGLGFQLPEAGDYGVGIVFLPD
                     NREQRQRFSKIIEGIVANEGQRLLGWRKVPTDNFYLGETAKFYEPFVRQIFIGRGSGI
                     DDDMAFERKLYVIRRRAENAIRYANLPGADFFYIPSLSHRTIVYKGMLTPRQVGTFYP
                     DLSDPMLESAIAVVHSRFSTNTFPSWGRAHPYRYLIHNGEINTLRGNENWMHARQSKL
                     ASHLFGDDLPKILPIIQEDGSDSAKFDNTLEFLALSGRSLPHAVMMMIPEPWENHDSM
                     DETKRAFYEYHSNLMEPWDGPASIAFTDGTVVGAVLDRNGLRPSRYYVTKDDLVIMAS
                     EVGVLDIAPENVAEKCRLQPGRMFLVDTGEGRIISDEEIKQQIAKAQPYGRWLKENVI
                     HFDQLPDVAEKEPVANHPATLQRLQAFGYNFEDLRINIAPMAQNGIQPVGSMGTDTPL
                     AVLSDRPQLLYNYFKQLFAQVTNPPIDPIREELITSTTLTLGLEGDLTDPRPESCRQL
                     RLSAPILKDSEVEKLRRIERPGIKSATLPIVFKASEGKGGLERALEELFALADRAISD
                     GATILILSDKGVDQFHAPIPALLACSGLHHHLIRTGTRTRVGLVLESGEPREVHHFCL
                     LLGYGVQAINPYLVFECLNDMIHEGMLKGLGYDGAVKGYIKAVVKGVVKVMAKMGIST
                     IKSYCGAQIFEAVGLGQELVNNYFTWTPSRVGGIGIDEVAREPQRQHDNAYPKYPLNG
                     HTLEVYGQYQYRTDGELHLFNPRTIHLLQKSCRTNDYKTFKEYSKLIDDQSQRLATLR
                     GLMQLKYAAQPISIEEVEPVEEIVKRFKTGAMSYGSISKEAHEALAIAMNRLGGKSNT
                     GEGGEDPARYVLDGNGDSRNSAIKQVASGRFGVTSYYLTQAREIQIKMAQGAKPGEGG
                     ELPGRKVYPWIAKVRHSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANHHARISVKL
                     VSEVGVGTIAAGVAKGHADVVLISGHDGGTGASPQTSIKHAGLPWELGLAETHQTLLL
                     NDLRSRITVETDGQLKTGRDVVVAAMLGAEEFGFATTALVTLGCIMMRVCHLDTCPVG
                     VATQNPQLRAKFAGDPAHVVNFMRFIAQEMREHMAKLGFRNINEIVGRADRLEVREAV
                     ENLKARGLDYTAILYQPKVGPDVGRYCSMPQNHGLENALDNQVLLDLAAPALERGEKV
                     KASLGIRNTNRVVGTILGSEITRRFGPEGLPEDTVHFHFEGSAGQSFGAFIPPGLTLE
                     LEGDANDYFGKGLSGGKLVLYPPEGSTFAPEENIIVGNVALYGATNGEAYIRGMAGER
                     FAVRNSGVRAVVEGVGDHGCEYMTGGKVFVIGDTGRNFAAGMSGGVAYILDEDGTFKT
                     RCNFETVALEPLNDRDLRDAEEMLKRHALYTHSARAWRLLALWEETAIKLRKVMPKDY
                     RRVLEELEKAEATGLAGEEALMAAFEANKNDASRVSGN"
     gene            complement(22414..22899)
                     /locus_tag="VF182_06790"
     CDS             complement(22414..22899)
                     /locus_tag="VF182_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX6436818.1"
                     /translation="MIDQIDLKILSILQNSGRSRLADIADEVELSAPAVLERVKKLEA
                     NGVIKGYQALLDGKKVGKDITAFIGVSIGNQQDINKFATQMLRNPDVLECHHITGDES
                     FILKVKSANTTSLEKLLGEIRSVEGVTRTVTRVVLSTAKESQMFDLTQGITESQNSKR
                     K"
     gene            complement(23166..23831)
                     /locus_tag="VF182_06795"
     CDS             complement(23166..23831)
                     /locus_tag="VF182_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436819.1"
                     /translation="MTATLKRPLPPGFPVPKQETERLLIERLQNSKNEEDYFRWLLFV
                     VAFYRGIEKVDSARALLQLFLETSKEPEQKAHCHLALGQIATDEQHIETALNHFTTAL
                     RFNPVKAKVGYVLHNNVSYCLNQLGRYRDAEQHCRMAIEIDARRASAYRNLGISLEGQ
                     QNTVAAVWALVEAIKLDSADDRAREILKRLISEHPLLTIQCPWIQEGFTPAHPDVENG
                     FAT"
     gene            24776..25087
                     /locus_tag="VF182_06800"
     CDS             24776..25087
                     /locus_tag="VF182_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX6436820.1"
                     /translation="MGSKLYVGGLPYSVTEGRLQEIFSAHGTVESANVISDKFTGQSR
                     GFGFVEMSSSSEAQKAIESLNGTQLDGRTLTVNEAKPMAKRDGGGGGGRGGRDGGRNR
                     W"
BASE COUNT         6076 a         6874 c         6622 g         6056 t
ORIGIN      
        1 gcataaagcg tctgatcggc gtattcccat ggcagtgaaa tcatgttgat cccatgtttg
       61 tctttgatct cgcgcgtcat attgcgcaat tctttgcggc ggatctgggc gccgccaatg
      121 cgcaacaacc cttcacggtc actgaccata gcttggatcg tctgcaagcc gtgacctgcc
      181 tgcggcttga caatccagag aaggcggccg ccggcgccat cgacggaaga catgtaggct
      241 tcgatgtccg atgccgattt cagtaaggaa agtacagtgg cagagccgga agttcttgct
      301 tccggcaccg ctaatccctt ctgactcaac ttaaaaagcg accggcgaat ttccttttta
      361 agcattttat cgtccgcttc cgttccgatc tgtgccaggg catcgacagc ggcggcttcg
      421 gcaattctcc ccaacgtaaa aacaatcgcc aagtctactt gacgatcctt gccgatttca
      481 ccctgtaaac gttggaccag agacggagac ggatcgtcca ccgcgatacc cgcctggcgg
      541 agcgcttcga gtccgctccg aatctccttc tcctgtgatt tgcttttagg caaagctcac
      601 ctgaattttt tatttcgcac tcatcagatc ggaaatcttg ccttgacgtc ccgcgaaagc
      661 gaaattcggc cacgtctcaa cctgcgcatc gcgatagttt tcccgatacc attgggcgat
      721 ctgaccggcg gtggcgaacc agacatcctt atgactcttt gcatagcgga taaattctcg
      781 cagtaacaga gccctaaacg gccggccgcc gacaaaggga tgattacccc acatcatgaa
      841 cttgggatcg ctggatcctt cctcatagag acagtcaaaa caatctttcc acatctgcaa
      901 gagatctcta ggggttctgc cggctcgctg gtagctcgag taatcattga gatcctggaa
      961 gtatgaaagg acagtcaatt cttttctccc caccttgagc gtatagggcg tttcctcatg
     1021 ctgcggatcc atccagtaac tatagccaag ctcgaggata atttcgggag tcgcgccgct
     1081 tggcgccacc ccggtggaaa gataaccgac cggcggtttg ccgaccacct tgctcaccgc
     1141 atcgacagtc ttctttaaat cacttttctc tttgtcgcgc tttttcatgt aactgtaatc
     1201 atgaatccag gtttccgtgc cgacttcgtg cccttgagcg gcgatctcct tgaatatctc
     1261 gggaaaattc tggaccgtaa taccggtcgg gaaaaaggtc gtgtgaattt tttccctctc
     1321 gaacacctcg agcatccgat agataccgac gcgctcgccg aattcccgat cagtgatgat
     1381 cgctaggttc cttttggtga cggcattggc tggaaaacta cggcggttct cctgttgcaa
     1441 cgtgcgcggc ccgcccagat cctccggcca cgtttcaaac gcgacgcacg gggtgatggc
     1501 aatgcgcgcg ttgtcgggcc atttgatcgg cgctctcttc ggtctaacct tcataatgcc
     1561 tcctcgaagt acaaaaattt cgttcagtca tgccacagtt tgttgcgctt gccaacacag
     1621 cctggatgct tggctcggga tcatttagtt gttgagcaat ttttttcaaa taagcgaaat
     1681 cacatgaatt atgaaggcgc tacgaaagat ccggccttga cttgaatagg catgccggtt
     1741 aagtgaatga gagcgaaata gagtgaggag gtttaccata tggcagaagg aaaaatgatt
     1801 gaatctcgcg ttgacaacgg cattgccgta ctcgaaatca atcggccgcc ggccaatgct
     1861 tacaccactg aactattgaa ggaattggat gccgcgattc tcgacgctcg tttcgacgat
     1921 aatgttcatg cgattgtcat aaccggcaag ctcgagcgat ttttttctgc gggggcggac
     1981 atcaagatgc tgggcagcgc aagtttatcg gtcaagaata atttcgctct tcacggtcat
     2041 gaagttctga tgcgcctgga gaacacgccc aaggtggtga tagcggccat caatggccac
     2101 gctcagggag gcggactgga aatcgccatg gcctgtgaca tacggatcgc caaaaaagaa
     2161 ggcggtcgaa tcggcctccc ggagattaat ctcggtgtaa tcccgggaat gggcggaacc
     2221 cagcgcttgc cgcgcctggt cggcaaagct cgagccatgg aactctgcgc gaccggaaag
     2281 ctcatctcct ttgatgaagg gctcgagatg ggactgatcc attacattta tgagaaggag
     2341 cacttcatgg aagaggttat ggattatgcg cgccagttca tcgtgcccaa caagagcagt
     2401 ctcgccgtcg gcaaggccaa aagagctatc acgacaggat ccgagctctc cctgccggac
     2461 ggattggctt tcgagcgcga gctcctggcg caaactttcg ccagtgaaga cggcaatgaa
     2521 ggcgtgaaag catttttcga aaaacgaacc gcgcaattca agggcaagta atcgaattca
     2581 aaaacaatca ttttcagatc tcaataagca actcacagcg cgatagatcg acgagccgag
     2641 tcgtactatg ttaggcacgc gatcgagcgc aacgaagcag gtgagtcttt ttaaacaatt
     2701 tgcattgaag gtaaagtaac tctatcaaac aaattcatta ccagcttaat aaatatttag
     2761 atgcgaggcg cgcgatggat cgacgagccg aggcgtactt cctgtacgtt gaggcgaggc
     2821 gattgagcgc aacgaagcag atgagtcatt tattaagcga tctgctggag gagagataat
     2881 ggcaaagatg tttatcggcg gagaaccggt cgattccgta acaaaagaga cctacgaaat
     2941 tcgcaacccc gctaccggcg cggtcgtgga ctcgatccca aagggaagtg agaaagacgt
     3001 tcagcaggcg atccaggccg cggaaagcgc attcaaagaa tgggcagatg ttacgccgga
     3061 agatcgcggc aactcgctca tgaatgcctg cgaattgatc aagaagaata ccagcgaaat
     3121 agcccaattg ctcacacaag agcaaggcaa aactttattc gaagcgggct tagagattca
     3181 ccatctggtg cacgggttgg agttctacgg tgggcttgcc tccaaggtgc gtggagcgca
     3241 tgtgccgctg ccgcaaaaag gcgcttacgg catggttatt cgtcaaccta tcggtgtatg
     3301 cggtggcatc gttccatgga acttcccgct gacattgatg ggcaccaagc tcggtccagc
     3361 gctggccgcc ggcaacagca ttatcattaa accggcaagc accacacccc tggcaacagc
     3421 tcgggtcatc gaactcattg cccaggccac ctttggcgaa ggcaaagcgc aaaaaacatt
     3481 gccgaaggga acagttaacg tcgtcaccgg gcccggcagc agtgttggtg aagagatttt
     3541 gcgtaacccg cgaattcgca ggattgcatt caccggttcg accccggtgg gccgtcatgt
     3601 catggaggtg gccggacgcg aaatcaaacg cgttaccctg gaactcggcg gcagcgatcc
     3661 catgatcgtc atggaagatg ccaatattga cctagccatc aagatggccg acgtcggccg
     3721 atactttaat tgcggccaga tgtgccttgg cgtcaagaga ttatttttgc aggaatcgat
     3781 agccgataaa ttcgttggcg gcctggtcga aatcttaaag aagaaaaccg tgggcaatgg
     3841 catgagcaaa gagagccgta tcggtccgat tcatctcgaa tcgcagcgaa aagaagtcga
     3901 agagcaggta gaagacgcga aatcgagggg cgccaagacc ctgttcggcg gcgagcggcc
     3961 caaaggcggg gacttcgata agggcttcta ttacacgccg accttgatga gcaacgtgcc
     4021 agaggacgcg cgaatgacca ccgaagaggt ctttgggcca gcgctaccgg tatacacgtt
     4081 taaaactctc gatgaggcgc tcgaaaaagc caactcctca gaatttgggc tgggttcatc
     4141 gatctggacc cgcaatcttt taagcgctaa caaagcaatc gataagttgc aggctggcaa
     4201 tgtttgggtt aactcgctcc attacggcta cgatgaactg ccgttcgggg gagtcaaatc
     4261 gagcggactc ggtcgtgagc acggtcccga ggcgctcgac tactatctcg aacccaaagg
     4321 cgtcgttatc gtcaatgttt gacgagtgat cgtcacatct taggccggag agattcccgg
     4381 cctaagatgt aactcttgcc ataactttgg caagcagcat cttccccaat cgagcagtgt
     4441 cataccgatc aaatctcaac acctcctcac ttgtagcggg caagatcgtt ggtagaattg
     4501 cttgcggcgg ttccacatga ccagcgaaaa ggatgaggaa tacttcgcat gcttgaaggt
     4561 ttccccggct cagatggatt ttctatgggc tgaagggtgg cggcacttcg gcatttactt
     4621 cttccgctat cacacctctt ttcacggcga taaaaagttt tcagttttgc cgttgagaat
     4681 tgatctggga cgattcactc tgacgcgcag tcaaaagcgt gtgctggcaa aaaaccggga
     4741 tgcgcggatt gtcgtacgac cggctgccgt cgatcccggt agacacgaac tcttcgataa
     4801 acaccgggtg cgctttaaag ataacgttcc cacttccctt gaagatatct tatcacccat
     4861 gcctgcgtcg gtgccctgcc cgagccgtga gctttgtgtt tacctggggg aacggttggt
     4921 tggtgcgact tatttggatc tcgggcacga tgctacttcc gctgtttacg caattttcga
     4981 cccggcggag gcaaaacgaa gtttaggaat actgatgatc ctgcaatcaa ttgagttctc
     5041 ccgagagcgc cgataccgat actattaccc tggttacgct tatcgcgagc ctttcgctta
     5101 cgattataaa aagagattta gcggattgca gtatttggat tgggatgcag gatggcgagc
     5161 ctacgagcac ggcagcaatc ccggcgatgc cctgtgaccg tctgcggacg atgacggcgc
     5221 ttcgcaggga ctctatgttc gagcgctatt tatccaaagt atagagcatc ttcaacatgc
     5281 ggttgacaat ccgttagtcg ttcgattagg ttagccgcat ctcgtccgga caaaggagcg
     5341 cgcctatgtt tgctcagatg aagcacatgg ccatcgtcag cagcaattgc aaccaggagg
     5401 gtgatttcta tcgcgatgtt ttcggcctga aacgctccgg actggctcgc gccggcggtg
     5461 ctgtggtcgt tcgtgatggt tatgtcgggc tcaacctcaa cccacgcgcg ccggggcgcc
     5521 aggcaggttt cgatcatttt ggttttgagg tcgaagatgt cgaaaccgtc ttctcccggg
     5581 tcaacgaaaa atatccaaag ataaaaattt taaaacgccc cggcaatcgt cccttcgccg
     5641 gcatcagttt gcacgatcct gcgggaaacg tattcgacct ttcacagaaa gggatggaga
     5701 atcggcgcga tctctacgtt gacgacgatc aaaccatgcc gcgccacgtc agtcatatcg
     5761 ctttgcgcgt tctcgatgcc gacggaattg ccgaattcta tcgcacggtg ttcgaactta
     5821 tggaattaga gaagcctgct ggagatccca atcattattt gtcagacggc cgagtcacct
     5881 tggctatctt cccgtggcag atcaccgatt acgaaggctc gggcatcgaa cggcccgctt
     5941 tggatcacat aggcttcaaa gttgaaagca tgccggcgtt caaagccgac ttcgacaaat
     6001 atatggcggc aaactcgcct gttattgctg atcgtccgga gaagaatccc gaaagagaag
     6061 tgaggcgtaa tctattgtcg aagtgttgct tcggacaatt cgaaatgtca gatcccgatg
     6121 gcgttttaat cgatgtttcg gaagcgtagg cggcgaatta ggattgggtc gcatgatagc
     6181 ggctccatct tcccacaccc aaacccaacg ctatcaccac tagaaagagc cccattgccg
     6241 gcagcatcgc cgactccggt ggctggactc cgtcgaccgc cagaacctgt tcggagcgca
     6301 ccgatctccc gtctggcaat tgcgcctgcg cgctaacccg gtgctcggca ccatcgacaa
     6361 agagaaattt catcgaaaac tcgccttcga cgggaattct atcgaccgcg aatacggtta
     6421 ttttcttttc gatgtgagtt attgcaaccg acagcaatgc tccgcggctt gcattgccac
     6481 catcggactc caggatccag cgaatctgcg caagcttgtc cgccgacggc ggatctattt
     6541 ccagccgcgt aattggccaa gccggagccg ctcgagcgag cgcggttggc gatggtaacg
     6601 cgaagaatag gctagcggaa aaaataatcg gcaacgctcc agcaccacgc ctactcaagg
     6661 catgagtgaa aattctgccg gcaaggacgc caaacccaaa cagcccgagt atgaaagccg
     6721 ctaaaagtag ccatttactt ctgtgctcac gaatatcgat cttgaacgtt ttactcgact
     6781 gcgtcccgtc cggcgccagc acctcaacga gcaggcgata ttcgccccgt atcgggaaat
     6841 tatatttcca ctcgactttt ccagctttaa gcggcaggcg cagctcggtg agtatgctgc
     6901 cttctgcgaa cggaaagtct gtcgagaaga aaatcggctg cggcgcttcg agtcgaagat
     6961 taagccatcc actggccaaa ggcttgccat cgacaccagt cgctaagagc gaaagagttg
     7021 ctgggtcgga aaacggcctt aaacgctctg gtactggcga agtctttaga taaatcttga
     7081 tgtcttgggc agagacacta gcaccggaga ggatgcaaag gatgaaaaat gcgggcgcga
     7141 agaaatactt cagtgacaag gaaccacggc tagagcgtgc ctaacattgt gcattgcatc
     7201 gtgcaccgca ggataatgag cggcaagagc gatccagaga atcgtgcccg acaatatcaa
     7261 taacatgccc gacttcatca tgaatgaaac cggctccgaa acagttacac cgagccattc
     7321 aacaaacgtt ttttccatat ggatacgctc ctcacctctt taatctaaat actaagactc
     7381 aaaaactgtc aagcgcacgc ttattcatca agtgcttgta agcccataat tctgcatata
     7441 agattcgcag aatcttatac cctctaggaa acactccgtg ctcagaagcg gactcggaac
     7501 gtgcatgcag ttcggtgtat ttgatcatct agatcgcaac gacctacccc tccgggagta
     7561 ttacgaggga cggcttaaac tggcggaagc gtacgatcat gcgggctttt acgcgtacca
     7621 tgtcgccgaa catcactcta ccccgctggg catggcgcct tcgcccagtg tttttttatc
     7681 cgccgtggct cagcgaactc atcggcttcg tttcggaccg ttggtttacg cgctgccgct
     7741 ttatcatccg atgcgtttga tcgaagaaat ttgcatgctc gatcagatga gcgggggccg
     7801 tctcgatatt ggctttggcc gcggcgcttc gcaaatagaa tccgccatgt acggcaacga
     7861 tccggcgaaa gccgagctca tgtacgtcga agggttggag ctcatcatgc gcgggcttac
     7921 tcagaagagt ctcaacttcc atggccagtt ttattctttc gacgacgtgc caatggagct
     7981 cgagccactg caaaagccgc atccaccagt ctggtacggg gttcactcac cggtgagcgc
     8041 cgaacgcgca gcgcgccagg gcctcaacat cgtcagtctt gatcccgccg ataagacacg
     8101 gacgtttacc gaccggtttc gccaggtctg gtgcgagagc gagaatagac aaacccgcga
     8161 gccaatggtc ggcctcggcc gttttatcgt cgttgccgca aaagacgaag aagcgctcga
     8221 tatcgcgcgc agggcgtatc caagatggca ccgcagtttc aactatcttt tcaacttacg
     8281 cggcggtaag ggaccggtcc accaacggcc tgcggatgta gagactctta ttaaaatagg
     8341 tcaaggcatt gccggtagcc caaatactgt aagcgagttt ctcaaagcgc agatgacaga
     8401 gtccggcgct aattacctcg tcggtcaatt tgcgttcggc gatctttctc tcagcgagat
     8461 attatcttct ctggaactct tcacacggca tgtgatgcct gaattgcgcg gcatcgacta
     8521 accccggccg agaaccagcg gcgcgccgac gccgatcatt tcagattttt ttcgaaaaat
     8581 tgagtcgtgc gctgccaggc gagatcggct gcctgcttat tatagttctg gcctttctcg
     8641 ttgccgaagg catggcccgc atcataccaa taggactcgc acgggacatt agcgcccttc
     8701 aatttggctt cgagcgcccg cgccgcttcc ggcgtaaaag acgcatcctt ttccgccaaa
     8761 tggagttgca aaggaatatt gatcttggtt acatccgcgg ctccttccgg aggaaagccg
     8821 taccaggatg aggctgcatc ggcttcagga acgaaaactg ccgccagcac agtcaaagcg
     8881 ccgcccatgc agaaccctac gacaccgact tttttactgg attgttttag atgttgcgct
     8941 gcgctccgga tatcctgccc agcggcatcg gcaaagttga gggttgtcat caagtgtttt
     9001 gcttcagcag catccagagt gacttttccc ttgtacaggt ctggaaccag ggcgcggtat
     9061 ccttgcgccg cgcaacggtc ggcgacagct ttgatctgat cattgagtcc ccaccattct
     9121 tggataatca ctaagcccgg cgcgttatcg ttgccggtct gactggcaag atagccgtta
     9181 cactcagctc cgtccggtcg cttaaacgca atcatctgtc ccatgagact acctccttaa
     9241 tggcagtgaa tttcttggtt actatcaaag cgatccctga agtcaatgtg gtgcgctcta
     9301 taacccgaaa gtcggaagtg ttaaactcgg gccatatgaa aattaaaaga tcgttgcgct
     9361 tgcatggggt tgcaatcctt gcccttggca tgttgacgat tactttgccg cagtcttggg
     9421 accttactgc ggctcaggag gtgacggatg agatccgcca ggagcagacc aaggcccgtc
     9481 agcagcaacg acttgaagag ttgcgccgtc aagagcagat cagacagctg cagcgagacc
     9541 aaaaaatcaa cgagcaacag cagcagctgg atcgaataca gcgacaggcg ccgcgctcgg
     9601 acactctgac gaagcaagat gcctccgggg tccagcagca attggatcag cagcagattg
     9661 agcagcagct acagcggcag cagaacgaga tgcagattga tcggattcaa agggagcagg
     9721 accagttgcg ccaacaacaa cggatcgatc agctgcagcg cgaacagcag atcgataatc
     9781 tgcgcaatca attaaggaga aatcaagtcc agcaggatct caatcgactg cggcaacaac
     9841 aaattcggcg ctgatacgtc gggatcagcc tgcttcaacc ctcgcaggac gcaccatgga
     9901 tcccctccat cacaaattgt tcgaccgcac acatgcgaac aatcagaacc gttgatacct
     9961 acctggtttc gtctcgcttc caggttttgt tttcacattg accgcgacag gaccctgtgg
    10021 tagcagcaaa ccatcgctca catgcgtcga acgctctcgc gaaaggtttt cacagtgatg
    10081 gtaaggcaac cgcaagaagc tattgctatg ccgtacggtg agcatttgct caccaaccac
    10141 gtaaggagaa acgatggaaa cagttggttt cgtaggtacc ggagcaatgg gaacggccct
    10201 actgtcgcgc ttacagctag tgccagtgtc ctctaaggca ttcgacgtct tgcccgcggc
    10261 gctggagcaa gcaaaggcac tgggcgctga gcccgcgaca tcggcaaagg cggttgccca
    10321 aagatcgact ctaatcgacg tggtggttcg caccgatcag gaagtgctcg attgtacctt
    10381 gggtgatgag ggcctcttgg aaggagccgc gccaggcact ttggtgctgc tacacagcac
    10441 gattcttcct accacgacca agctggtcgc ccaggcggct gcagataaac gcgtttatgt
    10501 gatcgatgcc tgcatgactg gagttccccg tgtggcgcga gacggcggac tgacgtttct
    10561 cgtcggcgga caaaaggcat tattcaaccg cgccaaaccg catctgctga agatgggcaa
    10621 agatgctgtt tacatgggtt ccttgggctg tggcaatgtg acgaaactga tcaagaatct
    10681 ggtcaccgcc tcggaagcgt tgatcgtcta tgaagccatt caaatcggca aggcggcggg
    10741 tatcgattac cgcgcggctt tggatttgat gaagaagacc caatcggagc catttctaag
    10801 tcgctgggac acaagattcg acgtctcagg atctgaactg aaatttaatt ccggtgctaa
    10861 cctttacgac aaggaccttc ccttggccgc tgaactcggc aacgcgctag gcgtcgagat
    10921 tccaatcacc gagcaactgg ccgaactagg cactcgccta agcggactcg atttggccgt
    10981 ctagtcctcc ttaaaacgtc aaactcttgc gactaaaagc tggcgcgacg atctgccgct
    11041 ggcatactta tgcggattag gaactatcga gaccacaaga tcgaactcct ctctgaactg
    11101 accaattctg gagaatgggt agctcaggca atgattgtca tcatcgttgc cggcaaagcc
    11161 aaacgaatcc cgatcttcgg ccgcaggcgc gccacgttcg attcgaaacg cggcgcagac
    11221 gcctatgctt tggaattggc caagctttgg gtcgatggcc gtttgtgggg cggcaacggc
    11281 cgcggataag attcagcaac tgagtgactc taagttcgta taatttgtcg ggaatcattc
    11341 tcatgccaag ccatcaagct ttttgtaggg gatcatcgat gcatgacgtt tcatgctgcc
    11401 ttattctgat gatgcttctc gtcctcttgc taaccagctg cgcaatcggg ccatcgacca
    11461 aggggtcgtc actgcacaat cctgcgcagc gagtttttct tgtgcggcac gccgaacgat
    11521 ttaacgattc acgagaagat cctccgttga cggccgaagg caaagaaagg gcaaaggcct
    11581 tagctgatat gctgcgcgac gccggtgtta gcgcaatcat cacctcacaa tggatacggg
    11641 ccaaagacac agcgcaaccc ttagccgatc tcctgaaaat tactcctgaa gtgatcccca
    11701 cagtcgatcc acctcacgag tatttccagg cgacggcgaa cgcagtccgg cgaataagga
    11761 acgatacagt tttggtcgtc ggccacatca cgattcccaa catcatcgcc gagcttgacg
    11821 gcccgcaact gccgacgatc tgtgaaagcg tgttttcgga tttgtttctg ctggtcccgg
    11881 cccttggatg ggacggcctc acgagattgc gttatggcgc tgcagagaac atttcgtcgg
    11941 gctgcagaaa tttacctgag tttctgcgcc cggactcaaa ttagcgacga caaaggagat
    12001 catttagtag gctaaccaat gccacagaca tttacgctcg cagcgggagc acacgacctg
    12061 aacaattcgc gaggtttctt ttctggcatc cagtcaattc gtggccatca aagacaaaaa
    12121 ggattgccta tcatcgtaac attccccagt ccccaagact ctctcaacca acggaattcg
    12181 tcacaatcgt ttgacatcgt ccatttcttc ttgcttgaat cgggagcatg gggcctcgat
    12241 ctgaaaccag ctcagcacta acaactcctc aaagaaacca accgcaatcc gacgggattg
    12301 cgatggcggg cccgtcctac ggtcgggaac tcgccgtgag aatgctgccg ccgattctcg
    12361 cgatcaccag tgtgctcgcc gtgtggtggg gcttatatct catttttccc cgattactgc
    12421 cgggtccact gagcacgttt gatgaagcta tgcgcttatt gtccgatggc actttctttt
    12481 ttcatatgct gcagagcctg cggcgggttt ttgtcggtgc cgcaatcgcc atgatcttca
    12541 gcatcgctat cggtatttac atgggaaccg ttctcatggg cgagcgtttt ttccaaccgc
    12601 tggtggtttt aggactcacg attccgggtt tgatgtgggc gctgatcgcc gtgatgctgt
    12661 tcggcatcaa cgagatcgct tcctatttcg cggtggcggt caccacattt cccatgctgg
    12721 taataaatat ctgggccggc gtaaagtcgc tcgataaaga tttgatcgac atgagccatg
    12781 tgttccgttt cacgccatgg atgaagatct ctcaggtcat tattcctcaa ctcgtgccca
    12841 atatttttgc ggcaacgcgc tacggcctgg ggttggcgtg gaaagttgtg gtagtggtgg
    12901 aaatgttcgg caccagcaac ggcgtcggtt atcaagtgat gaagtcctat caagtcttca
    12961 atatggaagg ggtgatcgcc tggaccctta cttttgtcgc cgccatgatc attattgaat
    13021 atggcatcat caacctggcc gagcggcgcc tgacggcgtg gcggccaaag gttgacgttt
    13081 ggagacgttg attggctcgt ttgatcgttc gcgatgtgga aaagtggttt cggcgcgagg
    13141 acgggcggcc gttaaagatt ctcgatcgtc tttcgttcga ggcgcttgag ggttgcgtca
    13201 cggtggtggt ggggccttcc ggctgcggca aatcgacgtt attgaacgcc atcgtcgggc
    13261 tggaacgctt agatcgcggt gaattggcgt ttgcaaagga cgatgttcat ttgcaacgcc
    13321 gtccgcttat cggttatgtg tttcaaagcc cgcgactgct tccatggaaa accgttgccg
    13381 ataatctcag acttgcgctg caaggggccg gcattgctcg aggcgagtgg caggagcggg
    13441 tggagcgata tttggatctg gttgcccttc gagaatacgc agaccaattt ccgctctatc
    13501 tttcgggggg ccagcggcag cgcgtagggc ttgcgcgagc tttggcgatc gaatccgatc
    13561 tcgtgttgat ggatgagcct ttcgcaagcg tcgatgagct gaccgcgcgt caattgcggc
    13621 aaaccacacg acggctttgc gagcagctgc atcgcacagt tctttttgtc acccacaact
    13681 tgcctgaagc ggcttatatg agcgattacg tagtgacgct tacaacccgt ccggcgcggc
    13741 tggcggaata cgtcaggaac cctttacccg cgccgcgaaa ctggactccg gagatctatc
    13801 ggtttgcggc tgagttggaa gccaaagtta ctttcaagga ggagagcgaa ctatgaacct
    13861 gtcaattatc atttccgcca ttgccatcat tgtcgaggta ggttcattcg caatggcgca
    13921 ggcgcagtcg cttcagacag tcaacatcgc aaccgctgcc gccggccagt ccggctatgt
    13981 cgcagcgctg atcgatgcta aaggcatcgc cgcgaaacac gggttaaaga taaacaatct
    14041 gatgatggac ttcacggaag cagccaacgc agtgaagttg ggacgggcaa tcgctagtgc
    14101 tatgcaaccg agcactgcgg tcaatctcct caaagccggc acggacatcc ggctgattac
    14161 gccacaaatt tggtccggca attcctggct ggtcaaaaag gattcgccct ataagtccct
    14221 ggcggatctt aaaggaaaga aaatcggaaa ttttgcgcga gttaccggag cgtatttttt
    14281 ctcggcggtg attgccaagg agaaaaagct cgatatcgaa aaggatttcc agagcattcc
    14341 agccgaaacc ggcgctctga ttgccttatt ggaacgcggc gaagtagaag cgatcaacat
    14401 gtttgagccg catacgacac ggctggtcct ttccgacaag taccgggtgc tgctcgattt
    14461 cgatatggaa ttggaaaaat tatttggcgc gccgccgctg aaatcatccg tcggcatgct
    14521 cagggaaacg gtcgaaaagc agccaggcct ggtgaaagcg attcgcggcg cctatgtcga
    14581 agcggtgaag gtgattaagg ccggccaaga taaggatttc ttcgaatcca aagccaaaga
    14641 atttttcaac ctcagcacgc ctgaacaagt tgctgcgggg ctgaaacaaa accgacaaaa
    14701 ttttgccgat atttggggtg agtcgttctt tcaatcacaa aacaaaattt tgcaatcagg
    14761 aatctcactg ggcttactgc cgaaggtcaa cttgaacgac ctatgggtaa aatgatttgc
    14821 gaagattggc aggctcttca gtccgtcagg cgatgggctc tgtatcgggt ctagcttcga
    14881 ttcgaaacga aactgttgcg ctgcaagtta gtgccggagg tttgcccctg gctgccgcga
    14941 cgcaaataca ttttgaccca atagtcataa aaatattgga ggttctgctt cgcttcttca
    15001 ggatatcctt cagccgtctc gaaacgaata gtgatccatt gctcctggag agaaattcca
    15061 atcagcgagc tattcttccg gccatcgaga taagctccgc tgcttcgcag tatatgtgac
    15121 aaactataag gatcgggcag agggctgatc ttggagcgtt tgcaacgagc gagatagtcg
    15181 aacttgacga tctcctcaag agtatagcgg acatcaacga ttcccgaacc caaagatcgg
    15241 cgaaaatccc catgcagcat ggcgtctaat cgcgtcatca agcgccgcga gaacggacca
    15301 ctgaagcgcg aatctccagt taatctctga atgccacgaa tgatataggc atccgcaaca
    15361 cagaccaaat cggcattagt gatttggcgc cgttcgagga gctgtcccac cactctaaga
    15421 tctcgagcgt aagaggacgc aggcgtttga tcaaaaccct ccgtctgcca gggggcttca
    15481 ccattgaagg ttaagtcggg agcaaccaga gtactcgctg ttttcattga tctagcctcg
    15541 caacaatctt tcgttttgag ttccgcgcca gagctatatc ggatttaccg gagcacgccc
    15601 cccgcaccat ctggtcgagc tcacatccca aagattgcct tcgcggaggt acttaacgca
    15661 acttgagggc cattagctac cgaacacaac ctcctggaaa ttcggcgaca tttctggttg
    15721 attgagcgat ctcttgtgcc agccattcaa cttgtctgac gtttttgaca attcacgacc
    15781 tcgagaaact cgatggttcg cggtgatgtc ggttcggcaa atggatgtag gaagaaccga
    15841 attaaagata gagcagtagc tggatctatc tgcgccgtgg cgtctcagtg cagctttcta
    15901 gcgtctgtct gccgcacgct tcacgttaac ttcgtcgtct ccatcaagaa gcgatcgcat
    15961 tctcgtgcag cgccgcgccc ctcgtttatc gcccatacga ccaggctctg accgcggcga
    16021 caatcgcctg cggcaaaaac cccggggacg ttcgtagaaa actccccgta ctcggccttc
    16081 gcattcgagc gtggatcccg ttcgacgcct agctgggcca acaccgtttc ctcaggaccg
    16141 acaaagccca tcgccaacaa tatcaaatcc gcgcgcaatt ttttctcggt cccgggtact
    16201 ctttttggaa tcaaggcgcc ctgctcgttt ttgacccatt cgacatcgta aacgtgcagc
    16261 tcttttacgc gtccgttcgc atccccgaca aatttctcgc cggttgttaa atagattcgc
    16321 ggatcatcgc caaaacgtgc tttcgcctct tcctgcccgt agtccaattt ataaacctta
    16381 ggccattcag gccatggatt atccggctgg cgtgtatcgg gtggacgcgg cagaatttct
    16441 aattgagtca agcttttgca tccgtgtcgc atcgatgtcc cgacgcaatc agtgccggtg
    16501 tcaccccccc cgacgacgat cacatctttg tcttttgcgg agatgtatct accgtctttg
    16561 tgattggaat cgagcaggct cttggtattt gaatggagaa aatccatcgc gaagtggatg
    16621 ccatcgagct cgcgcccggg tatcggcaag tcacgcgctt tggtcgcgcc gcagcagagc
    16681 acaatcgcat caaactcatc tttgagcttt tcagcgcgaa tatcgcggcc gaccgccgtg
    16741 ttcgtaatga atttgacgcc ctcggcagac aggagattga cacggcgttg gacgattgct
    16801 ttgtcaagtt tcatgttggg gataccgtac atcagcaatc cgccgacacg atccgcgcgc
    16861 tcgtaaacag tgacccagtg acccacatag tttagttgcg ccgcgcatgc aagccccgat
    16921 ggcccggatc cgatgatagc gacctttttg ccggtccgat tctccggcaa ttcgggtttg
    16981 acccagcctt gtgcaaaacc attatcgatg atggaacatt caatattctt aattgtcacc
    17041 ggcagttcgt tgagtcccag cacacacgac ccttcacacg gcgccggaca taccctaccc
    17101 gtaaactccg gaaaattatt tgttttgtgc aaccgatgta gggcctcctg ccagaggtcg
    17161 cggtaaacca gatcgttcca ctccggaatt aggttattga cggggcagcc agatgccatg
    17221 ccactgatca gctgtccggt atggcagaag ggaataccgc aatccatgca acgcgcgccc
    17281 tgctcctgaa gcttcgcttg tgaaagatgc tcgtgaaact cgcgccaatc gttcacccgc
    17341 tccaagggtg aacggtctcc aggtagctcg cgctcatagt ccaagaatcc ggttggttta
    17401 cccatattaa ttactcccga cgcatagcag atcgctgttt ggaacgatgt gccttatctt
    17461 gtccggttcg ctctaggctt gcttacttcg actggtttgt cgaaatcgcc acccgcatgc
    17521 ggcaaaatcg ccattccata ggatgacgaa agggaagagc cactggcagt ggcaccatga
    17581 gtgataatta gatttagttc cgaactgaat tatgaaatct ggcttaggaa ctcagttgcc
    17641 gctcactcgg gaagcatcgt tcttattggc ttcgaaagcc gccattagcg cttcttcacc
    17701 ggcgagcccc gttgcctccg ccttttcgag ttcttccagc acgcgacgat agtccttcgg
    17761 catcactttc cgaagcttaa tcgcggtctc ctcccaaagc gctaagagcc gccatgcccg
    17821 ggcgctatgc gtatatagcg catgacgctt gagcatttcc tcggcatcgc gcagatctcg
    17881 atcattcaaa ggctccaatg caacagtttc gaaattgcaa cgtgttttaa atgtgccatc
    17941 ctcgtccaga atgtaggcga caccgccgga cataccagcc gcgaaattgc gcccggtgtc
    18001 gccgataacg aagactttcc ctccagtcat atactcgcaa ccgtggtcgc cgacaccctc
    18061 cacgaccgcc cgcacaccac tgtttcgaac ggcgaagcgc tctcctgcca ttccccggat
    18121 ataagcctcc ccgttggtcg cgccatacaa cgcaacgttt ccgacaataa tgttttcttc
    18181 gggagcaaag gtcgaacctt cgggaggata cagaacgagt tttccaccag acaggccttt
    18241 gccaaaatag tcgttagcgt cgccctcaag ttccagagtt agacccggtg gtatgaaggc
    18301 tccgaaactt tgtcccgcag aaccctcgaa atgaaaatgc acggtatctt caggcaatcc
    18361 ttcgggaccg aatcggcgcg taatctcact gcccaagatg gtgcctacca cacgattcgt
    18421 gttgcgaatt ccgagagacg ccttcacctt ctcgccacgc tctaaagccg gcgcggccaa
    18481 atcaagcaat acctgattgt ccagcgcgtt ttcgagcccg tgattctgtg gcatgctgca
    18541 atagcgtccg acatcgggac caactttggg ctggtatagt atcgccgtat aatccaaccc
    18601 tcttgctttc aaattttcca cagcttcacg aacttccaac cgatcggccc gcccgactat
    18661 ctcgttgata ttacggaacc ctagcttagc catgtgctca cgcatttctt gcgcgatgaa
    18721 gcgcatgaaa ttaactacat gcgccggatc tccagcaaac tttgcacgca actgggggtt
    18781 ctgggtcgcc actcccacgg gacacgtatc tagatggcat acccgcatca tgatgcagcc
    18841 taaggtgacc agcgccgtag ttgcgaagcc gaactcttca gcacctagca ttgcagccac
    18901 gaccacatcg cgtccggtct tcagttggcc gtcggtttcc accgtgatac ggctgcgcaa
    18961 gtcattaagc agcaaagtct gatgggtttc cgctagcccg agctcccagg gtaagccggc
    19021 atgtttgatg ctggtctgcg gcgaagcacc cgtgccgcca tcgtgcccac ttatcaaaac
    19081 gacatcggca tgaccttttg ccacacccgc ggcgatcgta ccgaccccca cctccgagac
    19141 aagtttaacg ctgatccggg catgatgatt ggcgttcttg agatcatgaa ttagctgcgc
    19201 cagatcctcg atggaataaa tgtcgtgatg cggcggtggc gaaatcaatc cgacccccgg
    19261 ggtcgagtgg cgcactttag caatccatgg ataaaccttg cggccgggca gctcaccgcc
    19321 ttcgcccggc ttagcgcctt gagccatctt gatctgaatc tcacgagctt gagtaagata
    19381 ataactcgtt actccaaagc gtccggaggc aacctgcttg atcgcgctat tacgcgaatc
    19441 tccgttgccg tcgagaacgt atcgcgccgg atcttctccg ccctcgccgg tgttgctttt
    19501 cccgcccaga cggttcatgg caatggcaag cgcctcatgc gcttccttgc tgattgatcc
    19561 gtaggacatg gcgccggttt tgaaacgctt gacaatctcc tcgacaggtt caacttcttc
    19621 gattgaaatc ggctgggctg catattttag ctgcatgaga ccgcgcaggg tggctaaccg
    19681 ttgcgattgg tcatcgatta gcttgctgta ttccttgaat gttttgtagt cgttagtacg
    19741 gcaagacttc tgtagaaggt gaatagtgcg aggattgaac aaatgcagtt caccatccgt
    19801 gcggtactga tactgcccgt agacttctaa ggtatgaccg ttcaacggat acttgggata
    19861 ggcgttgtcg tgttgccgct gcggttcgcg agcaacttca tctatgccga taccgcccac
    19921 gcgcgatggc gtccaagtga aatagttgtt gaccagctct tgtcccaacc ccaccgcctc
    19981 gaagatctgc gcgccacaat aggatttgat cgtcgagatg cccattttgg ccatgacttt
    20041 tacgacgccc ttcaccacgg ctttaatata tcccttgacg gctccgtcat agccaagccc
    20101 cttgagcata ccttcatgaa tcatgtcgtt aagacattca aagacaaggt agggattgat
    20161 cgcttgcacg ccgtaaccaa gcaataaaca gaaatgatgc acttcacgag gctctcctga
    20221 ttcgagcacc aggccaacgc gtgtgcgtgt gcccgtgcgg atcaagtggt gatgtaatcc
    20281 ggagcaagca agcaaggccg gaatgggtgc atggaattga tccactcctt tatcggaaag
    20341 aatgagtatc gttgcgccat ccgatattgc ccggtcggct agagcaaata gttcctccaa
    20401 ggcgcgttcc aagccgccct tcccctcact cgctttgaag acaattggca aggtcgccga
    20461 ttttattccc ggtcgctcaa tccgccgcag cttctccacc tccgaatctt tcaaaattgg
    20521 cgctgacaat ctcaattggc ggcaactttc cggtcttggg tcggtcagat ctccttctaa
    20581 ccccagcgtc aatgtggttg aggtaatcaa ctcttcgcga attgggtcga tgggcgggtt
    20641 ggtcacctga gcaaacagtt gcttgaaata gttatagagc agttgaggtc tatccgacag
    20701 gactgccaaa ggcgtgtcgg ttcccatcga gcccaccggc tgaatgccgt tctgcgccat
    20761 cggcgcgata ttgatgcgta agtcttcgaa gttataacca aacgcctgaa ggcgctgtag
    20821 tgtagctggg tgatttgcca caggctcttt ctctgcaaca tcgggcaatt ggtcgaaatg
    20881 aataacgttc tctttcaacc aacgaccata tggctgagct ttggcaatct gctgtttgat
    20941 ctcttcgtcg ctgataatgc gtccttcacc ggtatcaacg agaaacatcc ggccgggctg
    21001 aagtcgacat ttctcggcga cgttctccgg ggctatgtcc aagacgccga cttcagaggc
    21061 cataatcacg agatcgtctt tggtcacata gtatctggat ggtcgaagtc cattgcggtc
    21121 gagcactgcc ccgaccaccg tcccgtccgt gaacgcgatc gacgcaggac catcccacgg
    21181 ctccatcaaa ttgctatggt attcataaaa ggcccgcttg gtttcatcca tactgtcgtg
    21241 attttcccag ggttcgggaa tcatcatcat gacagcatgc ggcagcgagc gcccgctcag
    21301 cgcgagaaat tctagggtgt tatcgaattt ggccgaatcg ctcccgtcct cctgaatgat
    21361 cggaagtatt ttaggcaaat catccccgaa caaatgcgac gccaacttgg attgtcgcgc
    21421 gtgcatccag ttttcattgc cccgcagcgt attgatttcc ccattgtgga tcaggtaacg
    21481 gtacggatgg gcccgccccc aactcgggaa agtgttggtg ctaaaacggg agtgcacaac
    21541 tgcaattgct gattccagca tcggatccga caagtctgga taaaaggtgc ctacctggcg
    21601 cggcgtcaac atacctttgt agacgattgt gcgatgagac agactaggaa tatagaaaaa
    21661 atccgcgccc ggaagattcg cgtagcgaat tgcattttcc gcccgtcggc ggatcacgta
    21721 caacttacgc tcaaaagcca tatcgtcatc gattccgctg ccacgtccta tgaaaatttg
    21781 tcgcacgaac ggctcgtaga acttagcggt ctctccgagg taaaaattgt ccgtgggcac
    21841 cttccgccat ccaagcagac gctgcccttc gttggccacg attccctcga taattttcga
    21901 gaaccgctgg cgttgttcgc gattgtccgg cagaaacacg atgccaacgc cgtaatcgcc
    21961 ggcctcaggt aattgaaatc ctaagccttc gcaagttcgc tcaaggaatt catgcggaac
    22021 ctgcagcaag ataccggcac cgtcgccggt gttttcctca caaccgcacg cgcctcgatg
    22081 atccaggttt tccaaaaccg tcagggcttg ttcaacaacc tcgtgagact tttcaccttt
    22141 gatatgtacg acaaagccga cgccgcaagc atcgtgctcg aaggtcggat cataaagtcc
    22201 ttgcttcggt ggtaatccgg ataatgtcat ggcttcacct ttgctccatt aactgcaatt
    22261 tcgatacgaa tccgattaac gtctcacaga gaattgcaaa tgaatccgaa agagatccat
    22321 gaacgtcgcc tgctgtcttc gcatcgtttg gttgatcgat agctcgcaat ctgaccggtt
    22381 attatgtcgg ccgtaaccga gcagtgctca tcatcatttc ctcttggaat tttgcgactc
    22441 cgttattcct tgagtcaggt cgaacatttg gctttcttta gcagttgata aaaccaccct
    22501 cgttaccgtc cgagtcactc cctcaaccga gcgaatctcg ccgagcaact tctccagcga
    22561 agtcgtattt gctgacttca ctttaagaat aaagctttca tccccggtta tatggtgaca
    22621 ttccaaaaca tctggattgc gcaacatctg tgttgcgaat ttattgatgt cctgctgatt
    22681 cccgatcgaa acaccgataa atgcagtgat gtctttgccg actttcttac cgtcgagtaa
    22741 ggcttggtat cctttgatga cgccgtttgc ttcgagcttt tttacacgct ccaatacggc
    22801 cggcgctgac agctcaacct cgtcggcaat gtcagcgagt cgagaacgcc cactattttg
    22861 gagaatagaa agtattttta aatctatctg gtcgatcatg ctaagaatct taggcgcaga
    22921 cacatctgaa cctaagattc ttatgtcaaa ggttctagga tgtgtcaagt ccgttcaagg
    22981 gcaagacaat atggacgatt ggaaactatg gtcgctacgg atggatgaag ccgtgcggga
    23041 gaaagcacac actaccgctt cgagatcaag taggtcgttt cagcgaaatg gacgagaaaa
    23101 cgattgaggg aaagacaact tcaaccatgg aacctgaggt aagcatgtta cggattattc
    23161 cggcgctatg ttgcgaaccc attttcaaca tcaggatgcg ccggagtaaa gccttcttga
    23221 atccacggac actgaatcgt caaaagtgga tgctccgaaa ttaacctttt cagaatttcc
    23281 cgagcacgat cgtcggcaga gtccaattta atcgcttcga ccaacgccca aacggccgcc
    23341 acagtatttt gttgtccttc aagactgatg cctaggtttc gataggcgct agcgcggcgc
    23401 gcatcgattt caatggccat ccgacagtgt tgttcggcat ctctgtaccg gccgagttgg
    23461 ttcagacaat agctaacatt gttgtgcaaa acatacccga cctttgcctt gaccggatta
    23521 aatcgaagcg cagtggtaaa gtgattcaga gcggtttcaa tatgttgctc gtccgttgcg
    23581 atctgcccca gcgccaaatg acagtgcgct ttctgttccg gttccttcga ggtttcgaga
    23641 aataattgca gcagtgcacg tgccgagtct accttctcaa taccgcggta gaacgccacc
    23701 acaaagagta accagcgaaa gtaatcttct tcattcttgc tgttttgaag gcgctcaatt
    23761 agtaatctct ccgtttcttg tttgggcacc ggaaagccag ggggtagtgg ccgtttcaat
    23821 gttgcagtca tgactgctcc tggagcaaca gccggaccaa tccgaagggg gattagatgt
    23881 gatcagaagg actaaaagct gtcgcttccc ggcgaaaaat aagtatcgaa atgctctgtt
    23941 agctcggcgg ctactgcaaa cgaggcgagc ggacccggtt tcggttcaca ggaatcgtaa
    24001 caattcacgg aaaggagaag ccatggtcag ggaaactgat ccagaacttg tggcccggac
    24061 ctccctttaa cgcgtgatcc acgcctaagc caacgaagcc atccagcgag gcgaaagctg
    24121 gagaaattgc tcggcctttg ccagcatatc tagattccgc aaagatcctt ggacattgaa
    24181 acaaatcgtc gtgacagctt cccttttttg ctgcacgagt cttctcagct acggatgttc
    24241 aggctttcat tatccacgcc gctattaccc ttaaaaatct tgcggtgttt ggaataactc
    24301 ggccattgca tttttattat ggtcggcagg gagtcaatga ggcctttgcc acagcgtggc
    24361 caccttggaa cggcaagcac catgagtgac ggccgatgct tttgccaacg cgctgaccct
    24421 cgcgcaatct attaggctcc gtcgaaacaa tttgaacgag gtctaacatc ggcttggatt
    24481 cgtcggtaag cccagggtgc gcgcctcaaa ctccaatgaa cagggctctc acgaagtcag
    24541 gcacgcgttg acatttgcgt tcacaccggt tagtgttagc tatcttcttc agtatcgggc
    24601 cttctggtag aggataataa tagttgaagg ctcatgtccg tctatctcct tgcgaacaga
    24661 tactatagga taatccgacc agagtgtggt tatgcagttt atcccccatg ttcggttccc
    24721 gttagcagtc aacttctcag ggtcagagac attaacattc tcttaggagg agaacatggg
    24781 tagtaaattg tatgtggggg gtttgcctta ttcggtaacc gaagggcgac tgcaggaaat
    24841 tttctcggcg cacggaacgg tggagtcagc taacgtgatt tcggacaagt ttaccggaca
    24901 gtcacgcggt ttcggctttg tcgagatgag ctccagcagc gaagcccaga aggctataga
    24961 aagcctgaac ggcacccagc tcgatggccg cacattgacc gttaacgaag ccaagcctat
    25021 ggcgaagcgc gatggtggtg gcggcggcgg gcgtggtggc cgtgatggtg gacgcaaccg
    25081 ctggtaatct gctcgccact cgattagcaa aaaagggcaa cccgaatggg ttgccctttt
    25141 ttgcgtcttg ccgcgcctga cggttagatt ctggtcaatt cggaccgacc gagcacgagc
    25201 agtggattgt acgatcaatt tccaagttcc aaatcaacac ggccccagcc aagtcttcgc
    25261 cgggcatctc atttcggaca tgcggatcca gaatacgtgc attgacagcc gctttgatga
    25321 gtcgacgctg cgatgattgg atcagcggga tctcggggcg gcattgttgt atttagcacc
    25381 cgcgtttgac agcccgaatc atttaagtcg attaagtcga tagctataaa atacttggtg
    25441 gtgccggttt gcttcgaacg cgaaaatacc ttctcgtcta agcatacctg ctgtccgaac
    25501 ccgcgcccgg attgtgacag cttcgtagca aaaacctatc tcacctcctg ctgacgtgat
    25561 aagctcatac atgattagtg ctaccctcag cttacgcacg tggaaacgct ccttttcatc
    25621 ggatgtga
//
LOCUS       DASUVS010000121        15714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_478690, whole genome shotgun sequence.
ACCESSION   DASUVS010000121 DASUVS010000000
VERSION     DASUVS010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 15714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15714
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478690"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..414)
                     /locus_tag="VF182_06805"
     CDS             complement(109..414)
                     /locus_tag="VF182_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX6436821.1"
                     /translation="MKRLRAIVLGISKDSIVSHTKFADKFNLPFVLLSDPKADVMKKY
                     QAFGRKMMYGKTVEGTIRSTVVVDPKGNVIKHWPTVKKADQHPQEVLAFLREQAGKN"
     gene            complement(455..589)
                     /locus_tag="VF182_06810"
     CDS             complement(455..589)
                     /locus_tag="VF182_06810"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX6436822.1"
                     /translation="MKSLEGQKAPAFSLEGHDGKRHSLEDYRGQKVILFFYPKDSTPG
                     "
     gene            complement(652..1452)
                     /locus_tag="VF182_06815"
     CDS             complement(652..1452)
                     /locus_tag="VF182_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX6436823.1"
                     /translation="MYLLNLATCMAESTESFCHGLAQYLEIRLGIPTQCVAGIPWQER
                     ERLFDQGAIQVLWLCGLPYVRKTQFKDFAIELLAVPIPLGARYRAEPIYFSDVVVKRE
                     SRFRSFYDLRGSSWAYNEPMSHSGFNVVRAYLAGFGYSQGFFREIVESGSHTASIEMI
                     LNDQVEASAIDTTVLDWVIDQRPEVETQIRVIETIGPSPIPPWVSSTQLPGHVRSALR
                     RTFLGMHEDNEGRAILDRGRITRFVAAHDTDYDPIRRMADVAASVSLV"
     gene            complement(1512..2870)
                     /locus_tag="VF182_06820"
     CDS             complement(1512..2870)
                     /locus_tag="VF182_06820"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX6436824.1"
                     /translation="MNRAYVPRVNIERARFEGRFRSAWAKTDELFRLINEDQMLSKPI
                     VWRHPFIFYVGHLPAFTWNQTCGALLDWQSFNPYFDDLFCRGIDPDVDTGECHWHPDV
                     PDTWPDLDPVLKYRDQVRSAVLEALDNMSRLESSDIMAQEGRAFEMVLEHEYMHQETL
                     LYMMHELPLDKKNRPAKSPRYSFQSAARSFPTEVPGGKVLLGAKFSELEFGWDNEFSQ
                     TVVDVPAFTMDSMPVTNGEYFEFVQSGAYDVEPYWRPEDWRWKVQTARKHPSVWLQRG
                     SLWLYKAMFDLFPLAQVSSWPVYVTVAEARAYASWRGKRLPSEAEFQRACYGGPDEIS
                     TRYPWGELAPAHRHGNFDFYSWSPDPVGSHPAGMSRWGIGELIGNGWELTDTPFAPFP
                     GFTPYMPRYPDYSKDFFDGKHFVVKGGSWATPVDLLRPTFRNWYQGHYPYVFAKFRCV
                     SS"
     gene            complement(2886..3353)
                     /locus_tag="VF182_06825"
     CDS             complement(2886..3353)
                     /locus_tag="VF182_06825"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX6436825.1"
                     /translation="MGNLEGQQAPAFTLEGSDGKRHSLSDYRGKSVVLYFYPKDDTPG
                     CTKEACGFRDSIDSVTNSNAVVLGVSKDGIESHNQFIDKYNLPFTLLSDPDTKVMQQY
                     GAWGERKNMEGKTVEGAIRSTVVIGPDGKVLRQWAPVANAEEHPREVLQFLQK"
     gene            complement(3473..4426)
                     /locus_tag="VF182_06830"
     CDS             complement(3473..4426)
                     /locus_tag="VF182_06830"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HEX6436826.1"
                     /translation="MVKIKGDLDLHKLEVFFWVAELKSFSEAAQRLSLKQPTVSAHIQ
                     ELEKTLGGKLFYRIPGKVTLTALGILLLEQAKDLLAFKRETVAKIERFHGSLSGELWI
                     GGSNIPGEYLLPQRLAAFIQKHPRVKPILRIGDSASIVDEVLDGKVEVGFVGFKNAEN
                     RLSFEKIWQDEMVLTVPSGHPWTRRKSVELSDLRSEQFISRERGSGTLDSFRHLISKG
                     RRSADDLLSVAMELGSTAAVKEALISGFGISILSRVSVERELADGLLTEIAIRGLKMI
                     RDFYEVCYKGRPLSPVAQAFREFLKADNSSKHYRHERRRIV"
     gene            4514..5623
                     /locus_tag="VF182_06835"
     CDS             4514..5623
                     /locus_tag="VF182_06835"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEX6436827.1"
                     /translation="MSKQSPLISVHHGNGAILADYDGWSLPRHFGNPHEEYLSVRRAV
                     GLLDFSNRAIFEFTGADRLSYLQGLISNDLRVASPGEGIYAAFLSQQGKVLGDCRVLT
                     TDDSFIVDLWEPLKHKIFDHLHRYLVADEVEITDLSDQCATLSIQGPKSETLLEEFLP
                     RNQQPQANLAHSITEVDGVEMRICRYSHTGEDGFDLIIPLAHIESMARQLTEIASTYS
                     ARWVGTEAYEILRIEAGIARYGADITEDNLILETGLTHAVSFNKGCYLGQEVVERIRS
                     RGHVNKNLTGLIIDGKKPPPAGSKIWSSEKEVGSITSSVYSPVFDSPIALGYVHRDHR
                     SRGTQLLVRYDAELLINATVTELPFTATRPGQRLT"
     gene            5747..6052
                     /locus_tag="VF182_06840"
     CDS             5747..6052
                     /locus_tag="VF182_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX6436828.1"
                     /translation="MANFVKVCRAEDIKAGCAKSLEINGKSIGVYNVDGKFYAINDIC
                     GHRGGPLAEGELDGTTVICPWHGWRYNVTTGENELLPALPIEKYEVKLEGDDVLVDV"
     gene            complement(6120..6746)
                     /locus_tag="VF182_06845"
     CDS             complement(6120..6746)
                     /locus_tag="VF182_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6447..6449),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX6436829.1"
                     /translation="MSRWKPLHSLPARFLGRISPLTARVMRSKITLSFTWKVRAAKPE
                     PALHSRRCKMAMPSIGQKAPDFTLPFSREQNVSLKDYQGSKNVVLAFYPLDFTGGUER
                     ELSSFEKNRKEFEARDAQVLSVSVDSVFSHKVFAERMGGINFPMLSDFWPHGAVCAQY
                     DCLRPQGMPKRAVFIIDKQGTIRFAKEYDKGVPENAELFSELDKINQD"
     gene            complement(6631..7170)
                     /locus_tag="VF182_06850"
     CDS             complement(6631..7170)
                     /locus_tag="VF182_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6436830.1"
                     /translation="MTADHQHHAHGHIEYRFCPRCGGGLEKRSIKAFEPKRLVCQVCS
                     FIFYQDPKVVAGTIFTLNGGVVLLRRGVEPAIGKWVFPGGYVDRGESVQEAAVRETLE
                     ESRLQVELDGLVDVYSYSKSPNVIIVYRAHIIGGELAAGDESVEAATFVASQIPWPDL
                     AFDSTRDALKDYIKLYLES"
     gene            7230..7913
                     /locus_tag="VF182_06855"
     CDS             7230..7913
                     /locus_tag="VF182_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX6436831.1"
                     /translation="MLRAVIFDFNGIIVDDEPIHFELFQRVLREEGIDLTEADYYERY
                     LGYDDRGAFSTAYREHNRALTRPKLGQLIERKAVYYRQDIKAKMKVFPGIQTLVPNLA
                     KQLPLAIASGALRNEIEIILSSIGLFTHFCAIVSAEDVTRGKPEPEIFLTALERLNAA
                     LDSNDPIRPAECLVIEDSKEGIRGARRAGMKCLAVTNSHPARLLDEADSIVDSLERIT
                     QKQLEAICS"
     gene            complement(7931..8650)
                     /locus_tag="VF182_06860"
     CDS             complement(7931..8650)
                     /locus_tag="VF182_06860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6436832.1"
                     /translation="MNSLVGKIAIVTGAGRGIGRAVATNLAEGGCRVILTARTSAQLE
                     QVQKEISTSGGETITIAADLTRDDDIQRLVQESREPWGAPDILINNAGWGKRAPVVKV
                     QREELDQTLRLNLRAPMILAQSFVPGMIEKGEGAVINIGSVSGKTGEANGAPYSASKF
                     GLIGFTQSLYEEVREHGIKVAVILPGFVDTPLIPPNRQLDRGKMIQPADVAQTVRYIL
                     DSAPTCCPVEITLRPQKSPYR"
     gene            complement(8915..9337)
                     /locus_tag="VF182_06865"
     CDS             complement(8915..9337)
                     /locus_tag="VF182_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6436833.1"
                     /translation="MQEEKLEVVRANESFYRAFESLDIKEMEKIWAKDCDIQCGHPGW
                     RILRGWEPVMESWRRIFENTPSMRFMLTDVSVDVRGSVAWVTLYENLTSSLEGQNISV
                     TILATNVFLKASGAWRMIHHHGSSVAPLGAQSDPSTVH"
     gene            complement(9343..9663)
                     /locus_tag="VF182_06870"
     CDS             complement(9343..9663)
                     /locus_tag="VF182_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6436834.1"
                     /translation="MFKSLTEVKQFAADKMKKNMLFGTDRFVCDVYCFEPGQIQAPHT
                     HDGQDKVYYVVEGRGLFEVDNEEKELGPGEMALAASGQNHGVSNRSQRQLVVLVFITP
                     NPSH"
     gene            9768..10922
                     /gene="thiI"
                     /locus_tag="VF182_06875"
     CDS             9768..10922
                     /gene="thiI"
                     /locus_tag="VF182_06875"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HEX6436835.1"
                     /translation="MLIRYHEIALKKGNRRYFIELLKRNLISAVKDLGLKEIRSLPAR
                     LLLTFKADYDTQVLIERIRSVFGVANFSMVQRTPRDIDILRARILESLNASRFSSFRI
                     DTQRGDKTFVLKSPEINRQLGAAVKEQSGARVDLTHAELTVTVEILPCDAFFGFNKIA
                     GPGGLPVGASGRVVALISGGIDSPVAAYRMMQRGCRLIFVHFHSTPYLDQTSQEKVRR
                     LVTELTRGQFQSRLYLIPFGEIQRQIVAGVARPLRVILYRRMMLRIAEQVARREKCKA
                     LITGESLAQVASQTLDNIAVIQQAAELPVLRPLVGMDKQEIIDQARRIGTYEISSIPD
                     QDCCQLFVPKHPATRARLADVEQDENKLDIEELLRLGTTQAEVEEFSFPQ"
     gene            complement(11142..12113)
                     /locus_tag="VF182_06880"
     CDS             complement(11142..12113)
                     /locus_tag="VF182_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436836.1"
                     /translation="MSRLKLTLACGDYDFLRPLINGELQPQGIDLNVLTMASPERHGR
                     MLRHEEFEVCELSLIAYLVSHDRGCNYTAIPVFPHRRFRHSYMVKRTGCSIDKPSDLN
                     GKRVALDTLQNSAGLWMRGILQDHYGVDLKSIEWWCQEEEDIAFEPAKWMNVKRVPPG
                     KNVDQMLLDGELEAALYPETLPSIKNGSPKVALLFPDPKKAEIEYYKNGGHFPIMHTV
                     VVKNEILEKNPWVGISLVRAFQRAKEICYERNSDPRSFALVWVQDLIREQRAIFGPDP
                     WPYNLEDNRRTLEAAIRYEYEQGMIRKTVDPEALFFAPSLQRIQHYV"
     gene            complement(12114..12704)
                     /locus_tag="VF182_06885"
     CDS             complement(12114..12704)
                     /locus_tag="VF182_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX6436837.1"
                     /translation="MIIAVVLSAGESSRMGRAKALLRIEGQRFIERIIGALGQSRVGR
                     TIVVLGHHAEQLRGQIEHLPVEIVINPDYQRGQLSSLQAAIRHIGNDDRCDGILVHLV
                     DHPFIDAALVNALIAKFLETKKLIVVPRYKGKRGHPVIFSRQLFQELLDAPLDQGAKA
                     VVNAHRPETVELEWQDEGITLDIDTPELYRQHVRGE"
     gene            complement(12721..13707)
                     /locus_tag="VF182_06890"
     CDS             complement(12721..13707)
                     /locus_tag="VF182_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436838.1"
                     /translation="MRIVRGIGLAIVFLLVLAAQSSSQERIKIAYSSADASNVVWFTA
                     LDAGLYRKHGLDVELVFIQSSTMSVSTLVSGDIQVANSSGGAVASAVVAGANLIMTAC
                     YINTLPYELIVQESVKSVEDLKGKSVGISRVGSASDVAARVLIKGLGLEPVKDVPIMQ
                     VGGPSERAAAFRTGKIVGFPSPPGTIHLAKGMPHRIMISTADFQKRFDFPYICSTTTK
                     TYLASNRETLRKLTMALIEATHFLKTRKQDTMKFIAKYTRQNNPQFLESSWAANVKLH
                     DRVPLVTREGTEVQIKEALARKPGATLRVEDIVDDSIVRELEKSGFIDQVYK"
     gene            complement(13848..14951)
                     /locus_tag="VF182_06895"
     CDS             complement(13848..14951)
                     /locus_tag="VF182_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6436839.1"
                     /translation="MIEVKSLVKIFADGKNAGVRAVDRVSFNVEEGRFYTLLGPSGCG
                     KTTTLRCIAGLEKADEGEIIVAGEKVFSASSRTFVPAYRRPIGMVFQSYAIWPHMTVF
                     ENVAFPLRVGKQKVSHDEIRRKVMTAVEQVELGGYEDRMATQLSGGQQQRLALARALV
                     REPKVLLLDEPLSNLDAKLRERMRVELRELQRRLGITTLYVTHDQIEALSMSNVIAVM
                     SAGVTVQEGPPREIYLQPRSKFVAQFIGSTNQISGQITNRGGDGIGSVRTEEGEISCG
                     ILTELKAGDKVVVVVRPESIVLHQKKPSHGENVLEGKIGAAMFLGEYVECTIELGRNV
                     LETHQPHSLRVRRGDTVWVELPVSDCMALPAEH"
     gene            complement(14965..>15714)
                     /locus_tag="VF182_06900"
     CDS             complement(14965..>15714)
                     /locus_tag="VF182_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6436840.1"
                     /translation="FVVLLPFLVLFWASFLPFFATPGWDAVEKLSFDNYRYLVGFRPF
                     WDALQNSIILATLTATVAMMLTSLIAWVVYKSRVPGSWLLDFLAFIPITVPGIVMGMA
                     LILLYVAFPLPIYGTIWLLLIAYVTRYMPYGMRSASGAIVQIHSELEEAASASGASWW
                     QTFKRVTLPLLRPGIAAGWIYICIVSFREFSTSVLLATGDSRVLSILLFTMFEQGQVT
                     IVAAIGILMIVVLLAIVAVFYKVSGRFGIQT"
BASE COUNT         3739 a         4357 c         3973 g         3645 t
ORIGIN      
        1 ccataaaatc cccctattgc ttttgccaat gctcttaatt ggtaattcct acaacggttt
       61 tttcagaaac acaatcagat aagaaccgca ccggctctga gtttggcgtt aattcttacc
      121 cgcttgctct cgtagaaaag ccagcacctc ttgcgggtgc tgatcagctt ttttgacggt
      181 cggccagtgt ttgatcacgt tgcccttcgg gtcaactact acggtggaac ggatcgtgcc
      241 ctccacggtt tttccgtaca tcatcttcct gccgaaagcc tggtactttt tcatgacgtc
      301 tgccttagga tcggacaaca gcacgaacgg cagattgaac ttatcggcaa actttgtatg
      361 agaaacaata ctgtctttgc tgatacctag aactatcgct ctcagccttt tcattttgcg
      421 tatttagctc gcggaacccg caagcttcct tggttcagcc cggagtactg tcctttggat
      481 aaaagaataa gatgaccttt tgaccccgat aatcctcaag cgagtgcctc ttaccatcat
      541 ggccttccag actgaacgcc ggcgctttct gcccctccaa actcttcatg gcgagatttt
      601 ctccctctat caagaatgta acatttgccc gaatcgatac gttgttagaa atcaaaccag
      661 ggacacgctt gcggcgacat cggccatgcg acgaatggga tcgtagtcgg tatcgtgtgc
      721 cgccacaaaa cgcgttatac gccctcgatc tagaatggcg cggccctcgt tgtcttcatg
      781 catcccgaga aatgttcttc gtaaggccga ccgcacatgc ccgggtagct gggtggacga
      841 cacccatgga gggatcgggc ttggaccgat cgtttcgatc acacggattt gagtctcgac
      901 ttcgggccgc tggtcaataa cccaatccaa cactgtcgta tcgatggcgg atgcttccac
      961 ttggtcattt aagatcatct cgatagatgc cgtgtgtgat cccgattcga cgatctctcg
     1021 gaaaaatcct tggctgtaac cgaaaccagc gaggtaagct cgcaccacgt tgaaaccgga
     1081 atgtgacatg ggctcgttat aggcccaaga ggaaccacgg agatcataaa aagacctgaa
     1141 gcgactctcc cgtttcacta cgacgtctga aaaatagatc ggttcagctc gatagcgggc
     1201 gcccagcgga ataggaacgg ccaaaagttc gatagcgaag tctttgaatt gcgtcttgcg
     1261 cacataaggt aatccgcaaa gccacagaac ctgaattgca ccctgatcga aaagccgctc
     1321 acgctcctgc caggggatcc ctgccacaca ttgggtcggg atcccaagtc ttatttccaa
     1381 gtattgcgcc agaccatgac agaagctctc tgtgctttcc gccatgcagg tggcgagatt
     1441 tagcaagtac atctgtcact gaatgcaggc atgcaaacat ttcatgtgac gaatcatgca
     1501 agttcttggc tctagctcga aacgcatcga aatttggcga acacatacgg atagtgacct
     1561 tggtaccaat tgcggaaagt cggtcgcagc aggtccaccg gagttgccca ggaaccgccc
     1621 tttacgacaa agtgctttcc atcgaaaaaa tctttggagt agtcaggata gcgaggcatg
     1681 tagggcgtga agcctggaaa cggcgcgaat ggcgtatcgg ttaactccca gccattaccg
     1741 atcaattctc cgatacccca tcggctcatg ccggctggat gcgagcctac ggggtcgggc
     1801 gaccacgagt aaaaatcgaa attgccatga cgatgcgccg gagctaactc gccccatgga
     1861 taacgggtcg atatttcgtc aggtcccccg tagcaggcgc gttgaaactc cgcctcgctc
     1921 ggcaatcgct ttccgcgcca gctagcgtag gcacgcgcct cagcaacggt cacgtagacc
     1981 ggccaactgg aaacctgggc gagaggaaag agatcgaaca tcgctttgta gagccagagg
     2041 ctgccgcgtt gaagccaaac actggggtgt ttcctggcgg tttgcacttt ccagcgccaa
     2101 tcttccggtc gccagtaagg ctctacatcg taagcgccgg actgcacgaa ttcaaaatat
     2161 tcaccattgg tgacgggcat agaatccatc gtaaatgccg ggacatcgac aaccgtttgt
     2221 gaaaactcgt tgtcccagcc aaactctaac tcagagaatt tcgcgccgag taaaaccttg
     2281 ccgcccggta cttccgttgg aaaagaccgt gcggcggact ggaaggaata gcgtggcgat
     2341 ttggctgggc ggtttttttt atcgagcggc agctcatgca tcatatacaa cagagtttcc
     2401 tgatgcatgt attcatgctc gagaaccatt tcgaaggccc gcccttcttg cgccatgata
     2461 tctgatgatt ccaaacgcga catattatcg agcgcttcca agaccgccga gcgaacctga
     2521 tcgcggtatt tcagaactgg atccagatcg ggccaggtat cgggaacatc cggatgccag
     2581 tgacactcgc ccgtatcaac atccggatcg ataccccggc aaaacaaatc gtcgaaataa
     2641 ggattaaagg attgccagtc caacagcgcg ccacaggttt gattccaggt gaaagcagga
     2701 agatggccga cataaaaaat aaaaggatgg cgccagacaa tcggtttgct tagcatctga
     2761 tcttcgttga tcaggcgaaa cagctcatcg gttttagccc aggccgaacg aaaccgtcct
     2821 tcgaacctcg ccctctcgat gttgactcgc ggtacgtatg cgcggttcat cacgacaccc
     2881 cgccgtcact tctggagaaa ctgcaatact tcacgaggat gctcctccgc gttagcaacc
     2941 ggcgcccatt ggcgtaatac cttgccgtca ggaccgatca ctaccgtcga gcgaatagcg
     3001 ccttcgacgg tcttgccctc catgtttttc ctctcacccc aagcgccgta ctgctgcata
     3061 actttggtat cgggatcgga aagaagagta aacggcaagt tatatttatc gataaattga
     3121 ttgtgcgatt cgattccgtc tttgctcacg cccaaaacga cggcgttgga attcgtgacc
     3181 gagtcgatgg aatcacgaaa gccgcaagct tccttggtgc atccaggcgt gtcatctttc
     3241 ggatagaaat aaagaacgac gcttttacct cgataatcgg acagagaatg tcgttttcca
     3301 tcgctgcctt ccaacgtaaa tgccggcgcc tgctgcccct ctaaattacc catgggaacc
     3361 tcctacttaa gatttgagct atttcttttc tctatcatct atagaggcgc ggtcaatacg
     3421 actagcgata attgtgcgtg gtgggagagc tggcggcacg cacggaggat acttacacga
     3481 tcctacggcg ttcatgccgg tagtgcttgg aagagttatc tgcttttaga aactcgcgga
     3541 atgcctgagc aactggtgat aacggccggc ctttgtagca cacctcgtaa aagtcgcgaa
     3601 tcattttcaa tccgcgaatt gcgatttccg ttaggaggcc atcggccagt tcacgctcca
     3661 ctgaaacgcg ggataaaatc gaaataccga accccgagat cagcgcctct tttactgccg
     3721 cggtgcttcc aagctccatt gctacactga gcaggtcgtc ggctgaacgt ctgcccttgc
     3781 tgatcaggtg acgaaaagaa tccaacgttc ccgagccgcg ttcacgcgag atgaactgtt
     3841 ccgaccgcaa gtctgacagc tcaacggatt ttcggcgggt ccatggatgt ccgctcggca
     3901 ccgtgagtac catttcgtcc tgccagatct tctcgaagga tagtctgttc tccgcgttct
     3961 taaagccgac gaatccgact tcgactttgc cgtcgagcac ctcgtcaacg atgcttgccg
     4021 agtcgccgat tcgcaggatg ggctttaccc gaggatgctt ttggatgaac gccgcaagcc
     4081 tttgcggcag tagatattcg ccgggaatgt tgctgccgcc gatccaaagt tcgccgctca
     4141 ggctaccgtg gaagcgctcg atcttagcca ccgtttctct tttgaaagcc agcagatcct
     4201 tcgcctgctc cagcagcagt attcctaacg cggtcagagt aactttcccg ggaatccgat
     4261 agaacaactt accgccaagc gttttttcca gttcctggat atgcgcgctg actgtgggtt
     4321 gcttcaacga tagccgttga gccgcttctg aaaagctttt aagctctgct acccaaaaga
     4381 agacttccag cttgtgtaga tccaaatcgc cttttatttt gaccatggta agttgatcgg
     4441 cggtgatgtt tgctattaaa tgcgccggga gcgaacgttg tcaaccgcgc aacttgacgg
     4501 tatattttga tcaatgtcca agcaatcgcc gttgatcagc gttcatcatg gtaatggagc
     4561 gatactagcg gattacgatg ggtggtcgct gccccgacat ttcggtaacc cgcacgagga
     4621 gtacttatcg gttcgccgcg cggtcggtct tttggacttc tccaatcgcg ccatctttga
     4681 attcaccggc gctgatcgtc tatcttatct acagggcctg atctcaaatg atttgcgggt
     4741 ggcatccccg ggtgaaggaa tttatgccgc gttcttgagc cagcagggaa aagtcctcgg
     4801 cgactgccgt gtcttgacca ccgacgatag tttcatcgtc gacttgtggg aaccgcttaa
     4861 gcacaaaatt ttcgatcatc tacaccgtta tctggttgcc gacgaggtgg agatcaccga
     4921 cttgagcgac caatgcgcta ccttgtcgat tcagggcccc aagtcggaaa cactgctaga
     4981 agaatttctc ccgagaaacc aacagccgca ggcaaaccta gcgcattcga ttactgaagt
     5041 tgacggggtt gaaatgcgca tctgtcgcta tagccatacc ggtgaagacg gtttcgatct
     5101 gataattcct ctcgcccaca ttgaaagcat ggcgcgccaa ttgaccgaaa tcgcctcaac
     5161 gtactctgcc cgatgggtcg gcacagaggc ttacgagatc ctgcgaatcg aagcaggtat
     5221 tgcccgttac ggcgccgaca ttaccgaaga caatctcata ctggaaaccg gtttgactca
     5281 cgctgtgagc ttcaacaagg gctgctatct gggtcaggaa gtcgtcgaaa ggattcgctc
     5341 ccgcggccac gtcaataaaa atcttaccgg gctcataatc gacggcaaaa agccgccgcc
     5401 ggcaggcagt aagatatggt ccagtgaaaa ggaagtcggc agcatcacga gctccgtcta
     5461 ctccccggtt ttcgactctc caatagcgct gggctatgtt catcgcgacc atcgttctcg
     5521 aggtacacag ctgttagtac gctacgatgc ggagcttctt atcaacgcca cggttacgga
     5581 gttaccgttc accgctaccc ggcccggcca gagactaacg tgatcgtgcc aatcgcatgc
     5641 gtcgggattc gaaccggcct gtccagaccc tccgcacttg aatccgaacg ccgagtccgt
     5701 atattgaacg aaaaattttg ccccacccga gaaaggctga agagaaatgg ctaattttgt
     5761 caaagtctgc agggccgagg atatcaaagc cggttgtgcc aagagccttg aaattaatgg
     5821 taagtcaatt ggcgtctaca atgtcgacgg caagttttac gcgatcaatg acatttgcgg
     5881 tcatcgcggc gggccgttag ccgaaggcga actggatggc accacggtga tctgtccttg
     5941 gcatggctgg cgctacaatg tcaccaccgg tgaaaacgag ctgttaccgg cgttgccaat
     6001 tgaaaaatat gaagtaaagc ttgaaggaga tgacgtgctg gtcgacgtct aggaaattat
     6061 ccgatacata aaaggccggc tgcatcatga tgcagccggc ctttgcttta tataagctat
     6121 taatcctggt tgatcttgtc gagctcgcta aacagctcgg cattttctgg aactcccttg
     6181 tcatactcct tggcgaatcg aatggttccc tgtttgtcga taatgaagac cgcacgcttg
     6241 ggcatcccct gcggccgcag gcaatcatat tgagcacaga cggcgccatg aggccagaaa
     6301 tccgatagca tcgggaaatt gatgccgccc attctctcgg caaatacttt atgtgaaaaa
     6361 accgaatcga cacttacgct cagaacctga gcgtcgcggg cttcgaattc cttccggttc
     6421 ttttcgaagc tgcttagctc acgctctcac ccgccggtaa agtcgagcgg gtaaaacgcc
     6481 aacactacat tcttgctgcc ctggtagtct ttgagcgaaa cattctgttc gcgcgagaac
     6541 ggcagggtga agtcgggcgc cttttgccca atgctcggca tcgccatttt gcacctcctc
     6601 gagtgtaaag ccggctcggg tttagcagct ctaactttcc aagtaaagct taatgtaatc
     6661 tttgagcgca tcacgcgtgc tgtcaaaggc gagatccggc caaggaatct ggctggcaac
     6721 gaatgtagcg gcttccaccg actcatctcc ggcggccagc tcaccgccga tgatatgcgc
     6781 ccggtaaacg atgatgacat tgggagactt cgaatacgaa taaacgtcga ccaagccgtc
     6841 caattcaact tggagtcgcg actcctccag cgtctcacgc accgccgcct cttggacact
     6901 ctcgccgcga tcgacataac cgccgggaaa tacccatttt cctatggcag gctcgacgcc
     6961 gcgccgaagt aatacaacgc cgccattcaa ggtgaagatg gtgccggcga cgaccttggg
     7021 atcttgataa aagataaaag aacaaacttg gcaaaccagg cgcttcggtt cgaacgcttt
     7081 gatcgatcgc ttctccagcc cgccgccgca acgggggcag aatcgatatt caatatggcc
     7141 atgcgcatga tgctggtgat ctgctgtcat agattctttt ttaccggata tttacttgaa
     7201 ggaaaagaaa attttgggta ccgtcaacga tgcttcgcgc tgtaattttt gatttcaacg
     7261 ggatcatcgt cgacgacgag ccgatccatt tcgagctatt tcaacgggtt ctccgagagg
     7321 aaggcatcga cctcaccgaa gcagactact atgagcgcta tctcggctac gacgatcgcg
     7381 gcgcattctc aactgcctat cgcgagcata acagagcact tacccgccca aagctcggcc
     7441 aactgattga acgaaaagcc gtctactacc ggcaagacat aaaggctaag atgaaggtct
     7501 ttcctggaat tcaaacgctc gtccccaatt tggcaaaaca actacctctt gccattgcat
     7561 cgggcgcgtt aagaaacgaa atcgagatca ttctctcaag catcggactg ttcacgcatt
     7621 tttgtgccat tgtcagcgcg gaggacgtca ctcgagggaa gccagaaccg gaaatcttcc
     7681 tgacagcact ggagcgccta aacgccgccc tcgatagcaa cgatcctatc cgtccggccg
     7741 aatgtctagt gatcgaagat tccaaggaag gaatccgtgg cgcccgtcgc gccggtatga
     7801 aatgtttggc cgtcaccaat tcgcacccgg cgagacttct tgatgaagcc gatagcatcg
     7861 tcgatagttt ggaaaggata actcagaagc aactcgaagc catttgctcg tagatgcgac
     7921 cggcgaaaaa ctaacgatag ggactttttt gcgggcgcag agtgatttcg acgggacaac
     7981 aggtcggagc tgagtccagg atgtagcgaa ccgtttgcgc cacgtccgcc ggttgtatca
     8041 tcttgccacg gtcgagttgc cgattcggtg gaattaatgg tgtatcgaca aagcctggca
     8101 agatcaccgc gaccttgatc ccgtgctcac gcacttcttc atacaaggat tgggtgaatc
     8161 cgatcaagcc gaacttcgat gccgagtagg gagcgccatt agcttcgccg gtttttccgg
     8221 aaaccgagcc gatattgatg actgctccct cacccttttc aatcattccg gggacaaacg
     8281 attgggcgag aatcatcggg gcgcggagat tgagccgcaa cgtctgatcc agctcctcac
     8341 gttgtacttt gacaaccggc gcgcgcttcc cccaaccggc attgttgatc aggatatcag
     8401 gcgctcccca aggctctcgg ctctcttgga caagccgttg aatatcatca tcacgggtca
     8461 agtctgccgc gatagttatc gtctcgccgc cggatgtgga tatttccttc tgaacttgtt
     8521 cgagctgagc cgacgtgcgg gcggttagaa tgaccctgca gccgccctct gccagattgg
     8581 tagcaacagc cctaccgatg ccgcggccgg caccggtaac gatggcaatt ttccccacga
     8641 ggctgttcac tggaataact acgaatcagc tgaataagag tacgggttca gtttagtcaa
     8701 gatctcattt ccgttgaaag atgactgact gatgatgtcg ttgtcgtctc actggatact
     8761 cgctgcagtg tgggctgaaa aatgccgctt ggaggctgct caaaaaggcc ttacatacga
     8821 gccgcgtaaa gtttctgagc tgcggcgtac gggcagtacg tttgaagcga gtcggacgat
     8881 cgcaacgaaa cattgaggct tttcagcagg cctgctaatg aaccgtcgaa ggatcgctct
     8941 gggctcccag aggcgcaacc gatgagccgt gatggtgaat catccgccaa gcgccggaag
     9001 ctttgagaaa aacattggtc gccaagatcg ttactgaaat gttctgaccc tcgagagaac
     9061 tggtcaggtt ttcatagagc gtcacccagg cgacgctacc gcggacgtcc actgagacat
     9121 cggtcagcat gaaccgcatg gagggtgtgt tctcaaaaat gcgccgccag ctttccatga
     9181 ccggttccca tcctctcaaa atacgccacc ccgggtgccc gcattgaatg tcgcaatctt
     9241 tcgcccaaat cttttccatc tctttgatgt caaggctttc gaaagcgcgg tagaagctct
     9301 cgttggccct gacgacttcc agtttctctt cctgcatcgg actcaatgcg atgggttggg
     9361 cgtgatgaac accaggacta ccagttgccg ttggctccga ttgctgactc cgtgattttg
     9421 tcccgaagca gccagcgcca tttcgcccgg gcctaattcc ttttcttcgt tgtcgacttc
     9481 gaacagaccc cttccctcga cgacgtagta aactttatcc tgtccgtcat gcgtgtgcgg
     9541 cgcctggatt tgtcccggtt cgaaacaata aacatcgcag acaaaccgat ccgtcccaaa
     9601 cagcatattt tttttcatct tgtcggcggc gaactgcttg acctcggtaa gacttttgaa
     9661 cataggtttt tcctaaatgg gtctatggaa ttgctgaccg gatactacca tagggataaa
     9721 aacatgcaaa accaggcgga atttgttatc gcttaaggat ggattcggtg ctgattcgtt
     9781 atcacgagat cgccttgaag aaaggtaatc gccggtactt catcgagctg ctaaagcgca
     9841 atctcatctc ggccgtcaag gatctcggcc ttaaagagat tcgcagcctg cccgcgcgcc
     9901 ttttactgac attcaaagcc gattacgaca cccaagtttt aatcgagcgc atcaggagcg
     9961 tcttcggcgt ggcaaatttt tccatggtgc aacgaacgcc aagagatatc gatattcttc
    10021 gcgctcgaat cctcgaatcg cttaacgcca gccgatttag ttccttccgt atcgataccc
    10081 aacgcggcga taaaacattc gttttgaagt cgccggagat caatcggcag cttggcgccg
    10141 ccgtcaagga gcagtcgggc gcgcgcgtag acctgaccca tgcggaattg accgtgaccg
    10201 tagaaatcct gccgtgcgat gctttcttcg gcttcaacaa aattgccgga cccgggggct
    10261 taccggtcgg cgccagcggc agagtggtgg cgctcatttc gggagggatc gattcccctg
    10321 tcgctgccta tcgcatgatg cagcgcggtt gccgtttgat tttcgttcac ttccacagca
    10381 ccccctatct cgaccagacg tctcaggaga aagtgcgccg gctagtcacc gagctgacgc
    10441 gtggccagtt tcaatcgcgc ctttacctga tcccatttgg agagattcag cgacaaatcg
    10501 ttgctggggt tgcccggccg ttgcgggtca tcttgtaccg gcggatgatg ctgcgaatcg
    10561 ccgagcaagt ggcgcgccgc gaaaaatgca aagccctgat taccggcgag agtcttgcgc
    10621 aagtcgcctc acaaacactg gataatatcg cagtgattca acaggcagcc gagctgccgg
    10681 ttcttcggcc gctggtggga atggacaaac aggaaatcat cgatcaggcg cggcgcatcg
    10741 gcacttacga gatctcgtcc attccagacc aggattgttg ccagcttttc gttccgaaac
    10801 atccagcaac ccgggcgcgg ctcgctgacg tcgagcagga tgaaaacaag ctcgatatag
    10861 aagaactcct ccgtttaggc acaacccaag cggaagtgga agagttctct tttccgcagt
    10921 gagcctctca gccgaatgcc gcagttaaat aagcgagggg taaacctgaa gcaatgaaaa
    10981 cttcgaagga ttcgttcaga gtccttttcc gagcttggcg cgcgattgca tgtgcacatg
    11041 cgtatttacg cagcgcgctg acagctgtcg cttctcagag gcggaaacca gtatcgccgc
    11101 tgcggaacgg ttgccctctc gcttctgatt ctcatcgagg cttagacata gtgttggatg
    11161 cgctgcagac tgggcgcgaa aaacaaagcc tcaggatcga ccgtttttct gatcattccc
    11221 tgctcgtatt cataacgaat cgccgcctct aaggtccttc tgttgtcttc cagattgtag
    11281 ggccaagggt ccggaccaaa gatcgcccgt tgttctcgga taagatcctg aacccagacc
    11341 agggcaaagc tgcgcggatc gctatttcgt tcgtaacaga tctctttcgc tctttgaaag
    11401 gcccgcacca ggctgattcc tacccacggg tttttctcga gaatttcgtt cttcaccacg
    11461 accgtgtgca tgatcggaaa atggccgccg tttttgtaat actcgatttc ggccttcttc
    11521 ggatcgggaa acaataaggc aaccttaggc gatccgtttt ttattgaagg cagtgtctcc
    11581 ggatagagcg ccgcttccag ctcgccgtcg agcagcatct gatcgacgtt cttccccggt
    11641 ggcacgcgct tgacgttcat ccattttgcc ggttcgaaag cgatgtcctc ttcttcctgg
    11701 caccaccatt cgatggactt caaatcgacc ccgtaatggt cctgaaggat gccgcgcatc
    11761 catagcccgg cggagttctg caacgtatcg agcgccactc tcttgccgtt cagatctgaa
    11821 ggtttatcga tgctacagcc ggttcgtttg accatatacg agtgacgaaa ccgccgatgc
    11881 ggaaaaaccg gaattgcggt gtagttgcag ccgcgatcgt gagacaccaa ataagcgatc
    11941 agtgacagct cgcaaacctc gaattcttca tggcggagca tccggccgtg ccgctctgga
    12001 gaagccattg taagcacgtt taagtcaatg ccctggggct gcaattcgcc attgatcaaa
    12061 ggccgtaaaa aatcatagtc gccgcaagcc agggtaagtt tcagtctgga catttattct
    12121 cctctaacat gttgtcgata taactcaggg gtatcaatat ctagagtgat gccctcatcc
    12181 tgccactcga gctccacggt ttctgggcga tgcgcgttga ccacggcctt ggcaccctga
    12241 tcaaggggag catcgagcaa ttcttggaat agttggcgcg aaaaaatcac cgggtgaccg
    12301 cgtttccctt tatagcgggg cacaacgatt aacttttttg tttccaagaa tttcgcgatc
    12361 aacgcattca ccagcgcggc atcaatgaat ggatgatcga ccaaatgcac cagtatgcca
    12421 tcgcagcgat catcgtttcc gatatgacgt atggccgctt gcaaggacga tagctgcccc
    12481 ctctggtaat cgggattgat cacgatctcg actggcaaat gctcgatttg gccgcgcaat
    12541 tgctcggcat ggtggcccag gacaacgatg gttcgaccta ctctgctttg gcctagcgca
    12601 ccaatgatgc gttcgataaa tctctggcct tcaatgcgca gtagcgcctt ggcgcgcccc
    12661 atgcgactgg actcgcccgc ggaaagaaca acagcgatga tcatgcgttt ttcggttacc
    12721 ctacttgtaa acctggtcga taaacccgct cttttccaat tcacgtacaa tgctatcgtc
    12781 gacgatatcc tcgacgcgca aagtagcgcc cggctttcgc gccaacgcct ctttgatttg
    12841 cacctcggtt ccttcacggg taaccaatgg gacacgatcg tgcaacttca cgttggcggc
    12901 ccaggatgac tctaggaatt gcggattatt ctgacgcgtg tacttggcga taaacttcat
    12961 ggtgtcttgc ttacgggtct taaggaaatg ggtcgcttca atgagcgcca tggtgagctt
    13021 tcttaaagtt tcccggttgc tcgccagata agtctttgta gtggtagagc aaatatacgg
    13081 aaaatcaaaa cgcttttgaa agtccgccgt gctgatcata atccgatggg gcattccctt
    13141 ggcaaggtga atggttccgg gcggtgacgg aaagccgacg attttacccg ttctaaaagc
    13201 cgccgctcgc tccgacgggc cacccacttg catgatcggc acgtccttca ccggctccaa
    13261 acccaatcct ttgatgagta ctctcgcggc cacatccgag gcgcttccca cgcggctgat
    13321 gccgacgctt ttccccttca aatcttccac cgattttacc gactcctgaa ctatcagttc
    13381 gtagggcaac gtattgatgt agcatgcggt catgattagg ttggcgccgg ctacgaccgc
    13441 gctcgccacc gcacccccgg agctgttggc aacctgaata tcgcctgaaa ccaaggtgga
    13501 gacactcatg gtcgagcttt ggatgaaaac cagctcgaca tcgagtccgt gtttcctata
    13561 cagccccgca tctagcgcgg tgaaccacac cacattcgat gcgtcggctg aactgtaggc
    13621 gatcttgatc cgttcttggc tagaggactg cgccgccaga actaacaaga aaactattgc
    13681 caaccctatc cctcgcacga tcctcatcga acctccaaaa tgcgcaataa attttctcta
    13741 gcgcgcaagc ctcgcatgga aacagttgaa aggggcgaca tcgaatgatg gcgacgcgag
    13801 tgacaagaac gggatgtaca acgaatgagg ccgcaacgaa tccaaaatca atgctcggca
    13861 ggcaaggcca tacagtcgct taccggcaat tccacccaaa cagtgtcgcc gcggcggact
    13921 cggagtgaat ggggctgatg agtctctaaa acgttcctgc cgagttcgat cgtgcactcg
    13981 acatactcgc cgagaaacat cgcggcaccg atcttgccct ctaggacatt ttccccgtgg
    14041 gagggcttct tttgatgcag gacgatgctt tccgggcgca ccacgactac caccttgtcg
    14101 ccggctttca attcagtcag gatcccgcag gagatctccc cctcctcggt tctcacgctg
    14161 ccgattccat cgcctccccg gtttgtgatt tgcccgctaa tctgattggt cgacccgatg
    14221 aattgcgcga caaacttact cctgggttga agataaatct cacgcggagg cccctcctga
    14281 acagtcaccc cggcgctcat cactgcaatg acgttggaca tcgacaaggc ttcgatctga
    14341 tcatgcgtaa catacaaagt ggtgatcccg agccgccgct gcaactcgcg cagctccacg
    14401 cgcatgcgct cgcgcagctt tgcgtcgaga ttgctcagcg gctcatccaa gagcaggact
    14461 ttcggctccc gtaccagcgc tcgcgccaac gcaagacgct gctgctgccc gccggaaagc
    14521 tgtgtcgcca tccggtcttc gtagccgccc aattcaactt gctcaacggc agtcatcacc
    14581 ttcctgcgaa tttcgtcatg actaaccttc tgtttgccga ccctcagggg aaacgcgaca
    14641 ttttcaaata ccgtcatgtg cggccagatg gcatagctct gaaaaaccat gccgattggc
    14701 cgtcgataag ccggaacaaa cgtccggctg gaggctgaaa agaccttctc tcccgcgacg
    14761 atgatttcgc cttcatcggc cttttccaag ccggcgatac atctaagcgt ggtagttttg
    14821 ccgcaaccgg agggtcccaa gagagtatag aatcttccct cttcaacatt gaaagagacc
    14881 cggtcaaccg cccttactcc ggcgttctta ccgtcggcaa agattttaac caaggacttt
    14941 acttcgatca cgactgcttt ttccttatgt ctgaatgccg aaacgtccag agactttgta
    15001 aaaaactgcc actatcgcca gtaacacaac gatcatcaag atgccgatgg ccgcgacaat
    15061 cgtcacctgc ccttgctcga acatcgtaaa cagaagaatc gacagcacgc ggctatcccc
    15121 ggttgcgagc aagactgaag tcgagaactc gcgaaacgac acgatgcaga tataaatcca
    15181 gccggcagca atgcccggcc tgagaagcgg taaagtcacg cgcttgaaag tctgccacca
    15241 tgaggctcct gaagccgagg cggcttcttc caactcgcta tggatctgca caatcgcgcc
    15301 tgacgccgag cgcatgccat aaggcatata gcgggttaca taggcgatca gcagcaacca
    15361 gatcgtgccg taaatcggta gcggaaaggc gacatagaga agaatcaatg ccatgcccat
    15421 gacgatgccg ggaacggtaa tcggtatgaa agccaaaaag tccagcagcc acgatccggg
    15481 aacacggctt ttatagacaa cccaggcaat cagagacgtc agcatcatcg caaccgtagc
    15541 ggttagagtc gcgagaatga tgctattttg aagcgcatcc cagaaaggcc tgaaccccac
    15601 aagatatcga taattatcga agcttagttt ctcgacagca tcccaaccgg gagtggcaaa
    15661 gaagggcagg aacgacgccc agaataagac caagaatgga agcaagacaa cgaa
//
LOCUS       DASUVS010000122         4640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_479243, whole genome shotgun sequence.
ACCESSION   DASUVS010000122 DASUVS010000000
VERSION     DASUVS010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479243"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VF182_06905"
     CDS             <1..318
                     /locus_tag="VF182_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6436841.1"
                     /translation="TDNRERLVKFYTTAFGMKVIDGVGPAIYLSDGYLNLAIIQKRGN
                     YKEGLYHFGFEVENIEELKPLCKDLGAATEIQKRPPNREAEYRVHDPDGTPIDLSQHG
                     WPV"
     gene            367..1932
                     /gene="ggt"
                     /locus_tag="VF182_06910"
     CDS             367..1932
                     /gene="ggt"
                     /locus_tag="VF182_06910"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX6436842.1"
                     /translation="MSSESIIHRPVIMGVHGMVATGHPLASAAALDVLRDGGNAVDAA
                     LCASAVLSVVKSYHCGLGGDVFGIFYLAKDRRVFALNGSGRSPRLLRRDTYSGSIPHR
                     GILAATTPGNVDAWFEAAAKLGSRAMGDLLKPAIEYADHGFPVFPHLAKVIRSSRNAL
                     GADPAWAAIFLPDGKAPDVGELLVQKDLSATLRSIASSGREAFYTGHIGRSIVEKSDQ
                     CGGFFSSRDFAEHQSKWEEPLSAIYRGYQIFVPPPNSYGLLLLLQLKYLADHDLAALG
                     HNTPECVALQVRAKEEAWRAGQSWLADPARYRPAEITKFLDSFPQKKESGDLAGGLLN
                     YGKSTTYIATADRFGNWASLIQSVHQSFGCGVVVDGTGIVLNNRMSGFNLVPNHPNEL
                     APAKLPAHTLSPALILRDDKPAIAVGTPGGLGQTQFLTQALCNIVDFGMNLQQAIEAP
                     RWQSESIGCVELENRMPSDVSQRLISEGYKVTVGAPWEFAFGGVEAIRLHDNGKVFMA
                     AADPRRDGYALGY"
     gene            complement(2309..3220)
                     /locus_tag="VF182_06915"
     CDS             complement(2309..3220)
                     /locus_tag="VF182_06915"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX6436843.1"
                     /translation="MSDSSLALVVDSYGLSDVGLVRTNNEDSFLIATPTGDFGNHNPA
                     EFNGMLPENGAMFIVADGMGGAQAGEVASRMAVDTVARNFIHTIQKTQPLDQQTLIST
                     LTETIQAANELIFLEGKKNSECNGMGTTLTAATVSGSTIFFAQLGDSRAYLARKGAIS
                     QMTKDQSLVAQMVASGSLSPEEAKNHPQRNVILQALGIQSQVDVAITLAELRKGDQIV
                     LCSDGLSGKVDAEEIREFLEKFEPKAACQGLVRMARERGGEDNITVIIARFNGDGLSD
                     PGPEDITESHNTKMEPARKRSFWPWRR"
     gene            complement(3217..4425)
                     /gene="icmH"
                     /locus_tag="VF182_06920"
     CDS             complement(3217..4425)
                     /gene="icmH"
                     /locus_tag="VF182_06920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HEX6436844.1"
                     /translation="MGEQQSSTWIHKGIANITRIGRANVQPVASAGGSNPLREIFTDL
                     FAFIVFFKSSCDKQPPDQNELREKILALATAQEERVKAAGISADSFREARFAVFSWVD
                     EMILTSTWPHRTRWQHLMLSYYKTFNAGEEFFARLQNLAPHANDVREIYYLCLSLGFL
                     GQYAFGDGPSEVKKLKQALYKQLSEKQGDMRRNYGLLFPEAYQKAISAARAPERAKFF
                     WYAMALITPVVLFIAYFVLLRYQADQLIAKIARVEQTPPTAAAIRKSWSGSLVEELKR
                     KGVRAAEQAEGVRITLDTVLFTAGSAQLNAQARAQIDDIVTTVKRYAPDKVVSIEGHA
                     SREREADEPRNRKLSEDRARTVAEAFTRSGFGSAQVFAQGLGSTKPVATNDTEHGRSQ
                     NRRVEITVKK"
BASE COUNT         1022 a         1213 c         1239 g         1166 t
ORIGIN      
        1 accgataatc gagaaaggct ggtcaagttt tacacgaccg cttttggcat gaaggtgatc
       61 gatggagttg ggccggctat ttatctgtcc gacggctacc tcaacttggc gatcatccaa
      121 aagcgcggaa attacaagga aggtctctat cattttggtt ttgaagtaga aaatatcgaa
      181 gagctaaagc ctctgtgcaa ggacctgggt gccgcgactg aaatccaaaa aagaccgcct
      241 aaccgggaag cggagtatcg cgttcacgat cccgacggaa ctcccatcga tctctcacaa
      301 catgggtggc cggtttaatc tttcgactat tatcgagcac tttggccagc agcggctcga
      361 tatgtcatga gctctgaatc cattatccac cggccggtga tcatgggtgt ccatggcatg
      421 gtcgccaccg gacacccttt ggcgtcggcg gcagcgctgg acgtcttgag ggatggcggc
      481 aacgccgttg atgcggcgct ttgtgcctca gcggttcttt cggtcgtcaa gtcctatcat
      541 tgcggcttag gcggcgacgt ttttggaatc ttctatttgg ccaaggatcg ccgcgtgttt
      601 gctctcaacg gcagcgggcg ctctccacgg ctgctgcggc gggatactta ttcaggaagc
      661 attccgcatc gcggcattct cgccgcgacc acgcccggga acgtcgatgc ctggtttgaa
      721 gcggcagcca aactgggtag ccgcgccatg ggcgatctct tgaagcctgc gatcgaatat
      781 gcggaccacg gttttccggt ctttcctcat ctcgccaaag tcattcgctc ctcgcgcaac
      841 gcgcttggtg ccgatccggc gtgggctgcg atttttctgc cggacgggaa agcacccgac
      901 gtcggcgagt tactggtgca aaaagatttg tcggcgactt taagaagtat cgcaagcagc
      961 ggacgagagg cgttttacac cggccatatc ggcagatcga tcgttgagaa atcggaccag
     1021 tgcgggggat tttttagttc gcgtgatttt gccgagcacc aatcgaaatg ggaggaacct
     1081 ctttccgcta tctaccgggg ttatcaaatc tttgtgccgc cgccgaattc ttacgggctg
     1141 ctgcttctcc tgcaattgaa gtatttggca gatcatgatc tggcagcatt ggggcataac
     1201 acaccagaat gtgtggcgct gcaggttaga gccaaggagg aagcttggcg cgcggggcaa
     1261 tcatggctcg ccgacccggc tcggtatcga cccgctgaaa tcacaaagtt tcttgattct
     1321 tttccacaaa aaaaagaaag cggcgattta gcgggcggtc tgctcaatta cggcaaaagt
     1381 accacgtaca ttgcaactgc ggacaggttc ggcaactggg cttcgctcat acaaagtgtc
     1441 catcaaagct ttggctgcgg ggtcgtcgtc gatgggaccg gaatcgtgtt gaacaaccgt
     1501 atgtccggtt tcaatctggt cccgaatcac cccaatgagc tagcgccagc caagctgccc
     1561 gctcacacct tgagtccggc gcttattctc agggatgata agcctgcgat cgcggtcggc
     1621 acgccgggtg ggttgggtca gacgcagttt ctcacgcagg ccctatgtaa tattgtcgac
     1681 tttggaatga accttcagca ggccatcgaa gcgccgcgct ggcaaagtga aagcatcggt
     1741 tgcgtcgagt tggaaaaccg catgcccagt gatgtgagtc agcgattgat cagcgaaggc
     1801 tataaagtga ccgttggcgc tccgtgggaa tttgcctttg gcggcgtcga ggccattcgc
     1861 ctccatgaca atggcaaggt attcatggct gcggcagatc caaggcgaga tggttacgca
     1921 cttggatatt agccgcaggg ggagccgtgg ttcttgattt aagctgtcgc gccccactgc
     1981 cacggaaatc aaaaacaaga ctatgactca gaactttgga aaggatttca gaaccgcgat
     2041 atatgcgcag ctcgtacggg actgtaacag cgcactcttt catcaggaac tcatgtgcga
     2101 gatcggccga ccgatcaaca atcaaacgtt cctttctatc gagtattctt gccgctggtt
     2161 tttttattca tgccagtgtt ttgtttttgg tcgagagcgc cgatttctgg catgtgcagt
     2221 ttctgaaggt aagtaccaaa ttgctcagaa acctgacggc accatttgaa actaaagtgc
     2281 ctaaccctcg atgccgatcg actggtgctt atctccgcca gggccaaaag gaacgcttgc
     2341 gcgcgggttc catctttgtg ttatgagact cggtgatatc ttcaggaccg gggtccgaaa
     2401 gaccatcgcc gttaaagcgc gcgataatta cggtaatgtt gtcttcgccg cctctttctc
     2461 gcgccattct cacaagtccc tgacaggcag cttttggttc aaatttttct agaaactctc
     2521 tgatctcctc ggcatcgact ttccctgaga gaccatcact gcataataca atctggtcgc
     2581 ccttccttag ttccgcgagg gtaatcgcta catcaacctg actctggatg cccaaagcct
     2641 ggagaataac gtttcgctga ggatggtttt tagcttcctc cggacttaaa gaaccggagg
     2701 ccaccatctg cgctacgagg ctttgatctt tggtcatttg ggagatggct cccttacgag
     2761 caaggtaagc ccgactatct cccagttggg caaagaagat ggtgctcccg gagacggtag
     2821 cagcagtgag cgttgtgccc atgccgttgc attcactgtt cttctttcct tcaagaaaaa
     2881 taagctcgtt tgctgcttgg atcgtttcgg ttagggtgga aatcagcgtc tgttggtcga
     2941 ggggctgtgt tttttggata gtatgaatga aatttcgtgc caccgtgtcg actgccattc
     3001 ggcttgccac ttctcctgct tgagcgccac ccatgccatc tgcgacaatg aacatggctc
     3061 cattctcagg taacataccg ttaaactcag cgggattgtg attgccaaag tcgccggtcg
     3121 gagttgcaat caaaaagctg tcttcattgt tcgtgcggac caagcccaca tcgctcaatc
     3181 catagctgtc tactaccaag gctaagcttg agtcgctcat ttttttacgg tgatctccac
     3241 gcgccgattt tgactccgcc cgtgttccgt atcattggtc gcgacgggtt tcgtgctgcc
     3301 aagcccttga gcaaaaactt gagcactgcc aaaaccggag cgagtaaacg cctcggccac
     3361 agtgcgcgcg cggtcttcgg ataatttacg attacgaggt tcgtctgcct ctctttctct
     3421 gctcgcatgg ccttcgatac taacgacttt gtcgggcgcg taccgcttga ccgtcgtgac
     3481 gatatcatcg atctgtgcgc gagcctgagc gttcagctgc gcgctgccgg ctgtaaaaag
     3541 cacggtatcg agtgtgatcc gtacaccttc cgcctgctca gcagcacgca ctcccttacg
     3601 cttcagctct tcaactagcg agccggacca agactttctg attgctgcgg cagtcggggg
     3661 cgtctgttcg actctggcga ttttggcgat caactgatcg gcttgatacc tgaggagaac
     3721 gaaatacgcg atgaacaaca cgacgggcgt gattagggcc atggcatacc aaaagaattt
     3781 cgcccgctca ggtgcccgcg ctgcactgat ggctttctga tatgcctctg gaaaaagaag
     3841 accataattc cggcgcatgt cgccttgttt ttcagagagt tgtttgtaga gggcttgctt
     3901 taatttctta acttcagacg ggccgtcacc aaaagcgtat tgtcccagga agccgagact
     3961 caagcacaaa tagtaaatct cgcgcacgtc attggcatgc ggcgcgaggt tctgcaaacg
     4021 agcgaagaat tcttccccgg cgttgaaggt tttgtaataa cttaacatta gatgttgcca
     4081 acgggtccgg tgcggccatg tcgaagtcag aatcatttca tcgacccagg aaaaaacggc
     4141 gaaacgtgct tcgcggaaac tgtcggcgga gatccccgct gctttgactc gctcctcctg
     4201 ggcggttgcg agcgcgagaa tcttctcgcg caactcattt tgatccggcg gttgtttgtc
     4261 acaggaggac ttgaaaaaga ctatgaaagc aaagagatcg gtaaagatct cgcgtaacgg
     4321 attgctgcct ccggccgacg caactggctg cacgttggca cggccgattc gggtaatatt
     4381 ggcgattccc ttgtgaatcc aggttgaaga ttgttgttct cccatgtaat tttagctgat
     4441 tatacgaagc tcttatcaat cgacgctcgc ctagaaatct ccagtgtttt taggtccggc
     4501 tgatcgatcg ttagatgaac ttacgatcga gcgaattcaa cgcttctgat ttccagtagc
     4561 gggcgttcgt ctccatcaat caaaaaggtt cgctgacctt tgccaaggtt gatttcttcc
     4621 ccgacattct cccaaactgt
//
LOCUS       DASUVS010000123        14459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1356296, whole genome shotgun sequence.
ACCESSION   DASUVS010000123 DASUVS010000000
VERSION     DASUVS010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14459
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356296"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VF182_06925"
     CDS             <1..300
                     /locus_tag="VF182_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6436845.1"
                     /translation="VPGVQAHHWTAGIAPTMSIAHKGAVVGAKVMAASVLDLLTSPEL
                     LAAAKKQFEQDTKDTKYFSLLPPGAKPPVDLNKDIMDRYRAAMSKFYLNKKAQFK"
     gene            complement(490..801)
                     /locus_tag="VF182_06930"
     CDS             complement(490..801)
                     /locus_tag="VF182_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436846.1"
                     /translation="MSKYLMNKLIHQVNMNQAMEDDYTSNPRAFVDKWEKGQKLKFTP
                     EEREALATKDYGKLYGLGAHPFTLWSFTEAVWIHDKPREELVADYKAKATQAGYPDFK
                     T"
     gene            complement(811..1083)
                     /locus_tag="VF182_06935"
     CDS             complement(811..1083)
                     /locus_tag="VF182_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEX6436847.1"
                     /translation="MDLRVLTTVFASVFIAELGDKTQLATMLFAADKETSRLTVFVGA
                     ASALVLTSAIGVVAGSVVSQYVSEKTLQYIAGVGFIGIGLWTLFKA"
     gene            complement(1105..1956)
                     /locus_tag="VF182_06940"
     CDS             complement(1105..1956)
                     /locus_tag="VF182_06940"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol ring-cleavage dioxygenase"
                     /protein_id="HEX6436848.1"
                     /translation="MAQIVTIIGITHNPFMPRLFQQPQQPPGAAKVKERIAMMRQKLS
                     DARPDVLITIGNDHLHQFFMDNMPAFMIGKMDQYDGTFYDEIREFGLPSCKLPGDPDV
                     SDAIMEGAFDRGVDFAYSNELTIDHSIIVPMLFVRPEMDIPIVPILTNCIAPPLPRPK
                     RFYEVGKAIRAAIDSLPPNKRVGVLVSGHLSLEVGGPKQFERKLQDPEFDGNAVGWIE
                     NGDIQSAADFCTPERMLRAGNMTMGFLNFLMMMGVANSSKPSYAEGLDAGFPAIPFFS
                     WDQQPSG"
     gene            complement(2097..3140)
                     /locus_tag="VF182_06945"
     CDS             complement(2097..3140)
                     /locus_tag="VF182_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6436849.1"
                     /translation="MKAIVLEEFGGPEVLRYHDVSVPRPAAGEVLLKVHSVSVNRTLD
                     LIVRAGQYPVAIEMPHVLGVDPAGEIAAMGDGVDSFQIGDRVAVVSFIACRRCKQCLK
                     GEEACCIASRHIGLHRWGGYADYVVVPAGNAFKLPDNVSYAQGSVIARHFPMAFNQLT
                     SKANVKPGHWVLVMGATGALGSCCVQVAKMLGARVIAGAGTDQRVELAKGYGADFGIN
                     YRRQDLLAEVMRLTGNEGVDVVCENISDPTLWAGAFNSLAINGTMVTAGAHGGGTVTL
                     DMKRLYMRRLRIIGTAGTNVADVYKALDAAAQGKIHAIIGRVMPLRDAAQAHRIVEKN
                     QIVGKIILDPTAS"
     gene            complement(3357..4334)
                     /locus_tag="VF182_06950"
     CDS             complement(3357..4334)
                     /locus_tag="VF182_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436850.1"
                     /translation="MKSSSTLALLAMILLTSPLYAQTKRIPVAYSAVSASQSSFYITK
                     EAGLFEKHGLFVDPVYVASGTKVAQAVIAGEFPIALAGGTVVNANLAGGDIAIFGGVV
                     NVPSFYVFVHSSIKRPEDLRGKTVGVTTFGSSTDFSIRYLIKKIGMEPDKDVKILQMG
                     GQPQIVAAMVAGAVQAGVLSSPANYNALKAGFQMLVDFKSVGLDYPTVSLVSTRSYIT
                     KDPQTVRRFLMAYSEGTERLYRDKEFAMKVIGKYTKTNEREALEAAYNFATTFVERPP
                     ELPYKAVETILTQLAEKDPKAKNFKPEDFIDATFFTELQKNGFFKSISR"
     gene            4505..5698
                     /locus_tag="VF182_06955"
     CDS             4505..5698
                     /locus_tag="VF182_06955"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436851.1"
                     /translation="MNIADIQAKSESWNIRLVGHHDLNGHGDGMQLLKKDRYVYIAHL
                     GTSPMALSIVDAADPAAPKVVRQMPHPPNTHAHKVQIAGDILIQNQERPYFDRNLPAD
                     VPNEAGIRIYNIANPTQPREIGYLKLPGKGVHRMWFTDGKYAHVAAMLPGIKERAYLI
                     ADISDPSNPKQAGVWWIPGTKNGEETPPDWTPFAGEHFHVHGAIPHGDRSYVALVDAG
                     MAILDISDISQPRTISHIDWSPPFGGYAHTTLPLPGRKLVVAVDESVKYDCNEGEKRV
                     WLIDVREERNPVIVSTCPVPEGDFCKRGLRFGPHNAHENRPGSFQSENLIFITYYNAG
                     LRIFDIRNQYRPEEVGFFIPPPPEGQKAPMFNDLYVDVDGLIYVTDRITGGLYILEYT
                     GPAIQ"
     gene            5778..6659
                     /locus_tag="VF182_06960"
     CDS             5778..6659
                     /locus_tag="VF182_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436852.1"
                     /translation="MSDRLLVNVFNVDAGLVVARAKGLFAAHGLEVDVMVTPNSTDQM
                     RGLSLGSWQIASTAFDNVLGWSGREGAEIVAIAQVAQGITLPVYVRPEIQTWEDLRGK
                     PLAVDAVDTAYALVLRRILLAHGLEMERGDYTLLPKGATGHRLESMQQGESFAGVLNP
                     PWDAKANAAGMKRFGDQRGILPDYPGGVFAVNRNWANDNRAILIKYLAAWDQGLHWAQ
                     DVKNRDEAIKLLAQAEKMDEASSANRLRQLPSNGQLNLAGLQTVLDLRVQFGLTPSMG
                     KDLLKYYDESFYNQTAK"
     gene            6704..7735
                     /locus_tag="VF182_06965"
     CDS             6704..7735
                     /locus_tag="VF182_06965"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6436853.1"
                     /translation="MKKLWTRFAGLWLGLMVAAWTNAAVAAPVDDLIAGAKKEGVIDF
                     YGPSTLGPEGAQAIVAAFNKKHGLNIKVNFTPSGNMTRDTAKIVGLSASGQPPEWDIM
                     VVTDAHHGSLWLRKLHKPFDYASLGVAKERVEYDNGTVSVANQFALPAYNTKLLPAKD
                     VPKTWEDLLDPKWKGKIGVINSTHHWGRLAAGAWGDEKTLDFIKKLSAQKPLLTRAGE
                     MAQRLILGEVLVSATLQDSQLHESKESGAPLAFAEQVSPVISPEYHVGVLKNAAHPNV
                     GHLFVTFMASADVQPVWQKYTGHTSAFVPGTNAYKFAQGKKVLYMKQDQAEKVDKISR
                     QIGKILGFD"
     gene            7771..9531
                     /locus_tag="VF182_06970"
     CDS             7771..9531
                     /locus_tag="VF182_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6436854.1"
                     /translation="MKAESRTVVSSNAWEGLSRFLPQGVTLFATGLSAFLLVLIGLPV
                     AMVILMSLRTGFPGEQVPFTLENFATVYLAPRTYEILLNTVFFAISSVSITLLIAVPL
                     VWILMRTDIPFKKTIYVLLTIGILIPVFLRTIAWILLLSPRIGLINKWLQQAFGLMEP
                     PFNLYSLTGMAFVQGVSFVPGAFFMLAAAYRSMDPSLEEAAYTSGVGKLRTFLKINIP
                     ITWPAIAAVMVYLIMTAVAVFEVPAIIGLPARIHVLSSLIFTSTTPSTGLPNYGMAGA
                     YGAIMLVLGLTLAFLYVRLVKQGKKYTVITGRGYRPREIALGRWKWAALAFVFFYLSI
                     EVFVPFSVLLWASLLPYLQLPSAEALSSLTLKHYIELPTHVGPRPFINTLILMFAVPT
                     ITMFLSVLVSWIVIRTRVSFRGFLDTLVFLPHAVPGILMAVGLAYLGLAYRDYFPLYG
                     TVSIIVLAHTINWIAYGTRTTNSVMIQVHRELEEAGRVSGASAPRVLGKIILPLIAAG
                     VLNSWIWIGMLSYREVTMSLTLLTRSNVVISTVVWQFWGSGWVPQVSALGVILILFAT
                     IVVGTVRFALSRIGEIGAAS"
     gene            9618..10697
                     /locus_tag="VF182_06975"
     CDS             9618..10697
                     /locus_tag="VF182_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6436855.1"
                     /translation="MISIRQLQKSFQAQQGRVDALKSIDLEVSEGEFCVLLGPSGCGK
                     TTTLRCVAGLERPDGGEIEISGRLVNSPRSRIFVPAEKRQIGMVFQSYAIWPHMNVFQ
                     NVAFPLTQGQKRVSKSSVAERVRAALKRVQLDGLEDRPATDLSGGQQQRVAMARAMVT
                     DSKVLLMDEPLSNLDARLREQMRVELRKITKSIGVTTLYVTHDQAEALSLGDKVCVMN
                     HGEILQIAQPNEVYARPVNLFVAQFVGEMNFVKAEVIDAGEVGCPLGRLPLSIPNGVT
                     KGANVTLAIRPEHVKVLPATHSAANRMPGKITSKNYLGDSALLEVEVNGTTLLAKLDG
                     DTELSMGDPAAVEFPAHRWHVFPQN"
     gene            complement(10874..11869)
                     /locus_tag="VF182_06980"
     CDS             complement(10874..11869)
                     /locus_tag="VF182_06980"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436856.1"
                     /translation="MKRIRPAKTLLWVAVIFYALPSLVAAQKINTSYISTTPGSSSVI
                     WIAKDAKIFDKHGFDATVIFISGSVRGIQAILAGEIPIGEGGGPGLASARLAGGDVVA
                     IAGNVNVLPYYLVSQPSIKKAEDLRGKIGGTHIAGTTAEFALKVGLKKIGLDPAKDVS
                     LRVIGGAIDRMVALQKGIVQFTVVTEAGKAIAEKLGYPTVVDMAALQIPFPQNGVYTS
                     TKLIRENPDMVRRYMRAYTEAIHFFKTRKEDTIKIMGKYSRLEDRKTLEEAWDWHAKF
                     IPEAPYAPVEGYQLILQDMAATNPKAAQANAKDLVDTRFVKELEDSGFIKSLYKK"
     gene            11991..12638
                     /locus_tag="VF182_06985"
     CDS             11991..12638
                     /locus_tag="VF182_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX6436857.1"
                     /translation="MKRSVDLIMFDLDGTLADTGPDLADSVNYTRSRFELSPLPQQLI
                     YTHVGRGVEHLLRHAVAKEAPSQFSEIMRVFLEHYQQHLLDKTTLYPHVHEVLDYFDG
                     KRNAVVTNKLHHLAVTVLRGLGIETRFDAVLGGDSGTEKKPDPALLRLVLRQFQIIPA
                     KALMVGDGDTDIQAGKWAGVITCGVTYGLANKDDLVAAKPDFLIDDLEQLTQYFC"
     gene            12665..13063
                     /locus_tag="VF182_06990"
     CDS             12665..13063
                     /locus_tag="VF182_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008416280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX6436858.1"
                     /translation="MADKIPEPFKDLFAKVAYANLATVMPDGSPQVTPVWFDYDGDCL
                     RINSAKGRIKDKNMRRDKRVALSIQDPDNAYRYLAVRGAVEEITEQGADAHIDSLAKK
                     YLGKDKYPFRGPGEVRVIYKIRPEKISTMS"
     gene            complement(13097..>14459)
                     /gene="sthA"
                     /locus_tag="VF182_06995"
     CDS             complement(13097..>14459)
                     /gene="sthA"
                     /locus_tag="VF182_06995"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HEX6436859.1"
                     /translation="QKAAIQGAKVGKKVGIVEQKKFVGGLCINSGTIPSKSLREAVLF
                     LSGFRQRNLYGASYRVKKDITFEDLLFKCDHVVKAEQEVVQNQLIRNHVDFIVGHASF
                     IEPHTLSVTQDSEIDEHTADFIVIAVGTEPSRPAGIPFDDATIIDSDGLLALNQLPKS
                     LTIVGGGVIGCEYASILATLGIPVVLIERRPRLLEFVDSELIEALQYQMRSIGITLRF
                     NEEVTAVQKHADGAVVIQLKSGKQIRAGLVMYSLGRVGATKRVNLESIGVQPDERGRL
                     KVNENYQTTLPHIYAVGDVIGFPALASTSMQQGRHAACHALGLACDTSTHLLPYGIYT
                     IPEISMVGRNEDELTRDGVPYEIGVARYREIARGQLIGDTVGMLKLLFHSDTRALLGV
                     HIIGEGATELVHIGQAVMALGGKLDYFIDAVFNYPTLAECYKVAALAALNRFPAIACA
                     PAI"
BASE COUNT         3299 a         3952 c         3854 g         3354 t
ORIGIN      
        1 gtaccgggcg ttcaagcgca tcattggacg gccgggattg ctccaaccat gtcgatcgcg
       61 cacaaaggcg cggtggtcgg agcaaaagtg atggcggctt cggttctcga cctgctcacc
      121 tcgcccgaac tgttggccgc cgccaaaaag cagttcgaac aggacaccaa agacacgaag
      181 tatttttcgt tactgccgcc gggcgcgaaa ccgccggtcg atttgaacaa ggatattatg
      241 gataggtacc gagcggcgat gagcaaattc tatttaaata aaaaagcgca gttcaaatag
      301 agcgtataaa acagaagagg gttagatcac aagaaacgcg ccggtggcaa cacaacccgg
      361 gctaagcgat ggactagacc gcttcaccac caagcgcacg aaggacacga aggtttccaa
      421 atcgaacctt gaatttctaa gcgtcgttcc ttgtggtgaa atcaaataga cccagttctt
      481 acgcactctt caggttttaa aatccggata tcccgcttgg gttgccttcg ccttgtaatc
      541 ggcgaccagt tcctctcgcg gcttgtcatg gatccacacg gcttcggtga agctccagag
      601 cgtgaacggg tgcgcgccca gaccatagag tttgccgtaa tccttcgtcg ccaacgcttc
      661 gcgttcttca ggcgtaaact tcagcttctg acccttctcc cacttatcga caaacgcgcg
      721 cgggttgctc gtgtaatcgt cttccatcgc ctggttcatg tttacctgat gaatgagttt
      781 gttcatgagg tacttgctca taaatttctc ctacgctttg aacaacgtcc aaagcccgat
      841 cccgataaaa ccgactccgg caatatactg cagcgttttt tcactgacat actgcgacac
      901 aaccgagccg gcgacaacgc cgatagccga tgttaagacc agcgccgacg ccgcgccgac
      961 aaaaaccgtc aatcggctgg tctctttgtc cgcggcaaat aacattgtcg caagctgggt
     1021 tttgtcgccc aactcggcga taaaaaccga agcgaatacc gtggtcaaaa cccttagatc
     1081 catcaacgct cccgtggaac tcactcaacc gctcggttgc tgatcccaac taaaaaaagg
     1141 aatggcggga aaacccgcat cgagtccttc ggcgtaggac ggtttgctgc tgttggcgac
     1201 gcccatcatc atcaaaaaat tcaaaaaccc catggtcata ttgccggctc ggagcatgcg
     1261 ctcaggcgtg caaaaatcag cagcgctttg gatatcaccg ttttcaatcc aaccgacggc
     1321 attgccatcg aactcgggat cctgcagctt gcgctcgaat tgcttggggc cgccaacctc
     1381 cagcgacaag tgcccgctga ccagaacgcc gacccgttta ttgggtggca agctgtcgat
     1441 agccgctcga atggctttgc cgacttcgta aaaacgtttc ggtcgcggca acggcggcgc
     1501 gatacaattg gtcaggatcg gcactatcgg gatatccatt tccggccgca cgaataacat
     1561 cggaacaatg atggaatgat caatggtcaa ttcattggaa tacgcgaagt cgacgccccg
     1621 atcgaaggcg ccttccatga tcgcatcgga aacatccggg tcgcccggca atttacacga
     1681 cggcagcccg aactcgcgga tctcgtcata aaacgtgccg tcatactggt ccattttacc
     1741 aatcatgaag gccggcatgt tgtccataaa gaattggtgt aaatggtcgt tgccgatcgt
     1801 gatcagcacg tcgggccttg catcgctcaa tttttgacgc atcattgcga tccgttcctt
     1861 gacctttgcc gcgcccggcg gttgctgcgg ttgttggaat agccgcggca tgaatggatt
     1921 atgagtgatg ccgatgatgg taacgatttg cgccatggct tacctcctgc tggtaattct
     1981 attggtgttt aacacggcta atttgccgct gtaaatggag aaagtagggc aggcacaggg
     2041 gcttgccccc tacaacgtag ttgaactgac gttgaacgga aaaaaatagg ttgagactag
     2101 ctcgccgtcg gatccagaat gatttttccc acgatctggt ttttttcaac gatccggtgc
     2161 gcctgagccg catcgcgcaa aggcatgaca cgaccgataa tagcgtgaat cttcccttgc
     2221 gcggccgcgt caagcgcctt gtacacgtcg gcgacattgg tgccggcggt gccgatgatg
     2281 cgcaagcggc gcatgtacaa acgtttcata tccagagtta cagttccgcc accatgagcg
     2341 ccggcggtga ccatggtgcc gttgatagcc aggctattaa acgctccagc ccagagcgtt
     2401 ggatcggaga tgttttcgca aacgacgtcg acgccttcgt tgcccgtgag cctcatgact
     2461 tctgcgagca aatcctgacg acgatagttg atgccgaagt cggcgccgta gcctttcgcc
     2521 agctcgactc tttggtctgt gccggccccg gcgatgactc ttgcgcccag cattttggcc
     2581 acttgaacac agcaactacc caacgcgccg gtagcaccca tgactaacac ccagtgcccg
     2641 ggtttgacgt tggccttgct ggtcaattga ttgaatgcca tcggaaagtg gcgcgcgatc
     2701 acgctgccct gcgcgtatga gacgttatcc ggcaacttga acgcattgcc cgcggggaca
     2761 acgacataat ctgcataccc gccccagcga tgaaggccga tgtgtcggct cgcaatacag
     2821 caggcctctt cccctttgag acattgcttg catcgacggc aggctatgaa ggaaacaacc
     2881 gccacgcggt caccgatttg gaagctgtca acgccgtccc ccatggccgc gatttcgccg
     2941 gccggatcaa caccgaggac gtgcggcatt tcaatcgcaa cgggatactg accggcgcga
     3001 acgatcagat ccaacgtccg gttgaccgaa accgaatgga cttttagtaa aacctcacct
     3061 gccgctggcc ttggaaccga aacgtcgtgg tagcgcaaaa cctccggacc accgaattct
     3121 tcaagcacga tggctttcat tgcttcacct tgtcgcttgt cagcttacga atcgaagcgg
     3181 cggagttcaa ataggccgtg gcttgctcga gggtctttgc gccgcatcta cgattctcga
     3241 tgtactactg aagctgatcc aggtgaaaaa tgtcgttcga gcgggttcga tcgtccattg
     3301 cagccactcg caaacgaggc atgtcggttc tgcaggttag cccttactcg gcccccttag
     3361 cgcgagatcg acttgaagaa accgttcttc tgcagctctg taaaaaatgt cgcgtcgata
     3421 aaatcttccg gtttgaaatt cttcgccttt gggtctttct cggcgagctg cgtcagaata
     3481 gtctccactg ccttgtaggg caattccgga gggcgttcga caaacgtggt ggcaaaattg
     3541 taggccgctt cgagcgcctc gcgctcgttg gttttggtat atttgccgat gaccttcatg
     3601 gcgaactctt tgtcccggta caggcgctcg gttccttcgc tgtaggccat cagaaacctc
     3661 cgcaccgtct gaggatcttt cgtgatatac gagcgggtcg aaacaaggct tacggttgga
     3721 taatcgaggc cgacgctttt gaagtccacc aacatctgaa agcccgcttt cagtgcatta
     3781 taatttgccg gtgacgagag cactcccgcc tgcacggcac cggcaaccat ggcagcgacg
     3841 atctgcggct gcccgcccat ttgcagaatt tttacatcct tatcgggctc cattccgatc
     3901 ttcttgatca agtaacgaat cgaaaaatcg gtcgatgagc caaacgtggt gactcctacg
     3961 gttttccctc taagatcctc cggtcgcttg atcgaagaat gcacaaaaac ataaaacgaa
     4021 ggcacgttga caacgccgcc gaaaatcgca atgtcgccgc cggctaaatt ggcgtttact
     4081 accgttccgc ccgctaaggc gatcgggaac tcacccgcga tcactgcctg agcgactttt
     4141 gttccggagg cgacataaac cggatctacg aacaagccat gtttctcgaa gagcccggct
     4201 tctttggtta tatagaaaga cgattgcgac gcgctgaccg cgctataagc caccggtata
     4261 cgctttgtct gcgcataaag ggggctggtc agcaggatca tcgcaagaag cgcgagcgtc
     4321 gaacttgatt tcatagatcc ttcctcgttt cggtggttac actctaacca tgcggctctt
     4381 aacatataaa aaagaatagg ccaacagtcg tagggtcgtc gttgacattc gataatcgga
     4441 agtataacag ttccaggcgc gatgcctgat tgccaattaa attttcctga gaggccgaga
     4501 gccaatgaac atcgctgata tacaggcgaa aagtgaatct tggaacatcc gtctagtcgg
     4561 ccatcatgat ctgaacggtc atggcgacgg catgcagctg ctaaagaagg atcgatacgt
     4621 ttacattgct catctcggaa cctcgccgat ggcgctgagc atcgtcgatg cagctgatcc
     4681 cgccgctcca aaagtggtgc gccagatgcc gcacccgccc aacacccacg cccacaaggt
     4741 gcaaatcgcc ggagatatct taatccagaa tcaagagcgc ccttatttcg atagaaatct
     4801 tccggccgat gttcccaacg aagccggcat tcgcatttat aacattgcca atcccacgca
     4861 gccgcgcgag atcggttatc tcaaactgcc gggaaaaggc gtgcaccgca tgtggttcac
     4921 cgacggcaag tacgctcatg tcgcggcaat gttgccgggc atcaaagaaa gggcttatct
     4981 cattgcagac atatccgatc cgtccaatcc aaagcaggcc ggggtctggt ggattcccgg
     5041 aaccaaaaat ggagaagaga cgccgccgga ttggacgccg tttgcgggcg agcactttca
     5101 cgtacacggc gctattccgc acggcgatcg ttcgtatgtc gcactggtcg atgccggcat
     5161 ggcgatcctg gacatttccg acatcagcca accaagaact atcagtcata tcgactggag
     5221 cccgccgttt ggcgggtatg cgcataccac gctgccgttg cccggaagga agcttgtcgt
     5281 cgccgtcgat gaatcggtga aatacgattg caacgaggga gaaaaacgcg tctggctgat
     5341 cgacgtgcgc gaggagcgta atcccgtcat cgttagcacc tgcccggtgc cggaagggga
     5401 tttttgcaag cgcggcctac gattcggccc gcacaacgct catgagaacc gacccggctc
     5461 gtttcaaagc gaaaacctta ttttcattac ctactacaat gccgggttgc gcatcttcga
     5521 tatccgtaat cagtaccgcc ccgaagaagt cggctttttt atcccgcctc cgcctgaggg
     5581 gcagaaggcg ccgatgttca acgatcttta tgtcgatgtc gacgggctaa tctatgtaac
     5641 cgaccgaata accggcgggc tatatatatt ggaatatacc gggcccgcga ttcagtgaga
     5701 gcgatcttcg ctaaaccgat tttagcgctt gctgcggatc catggctcaa ggatagcgat
     5761 caggagtctc ttttcttatg tccgaccgtt tgctcgtaaa tgtcttcaac gtcgatgccg
     5821 gcttggtggt tgcgcgggcc aagggcttat tcgctgcaca cggactggaa gtcgatgtca
     5881 tggtcacgcc caactcgacc gatcagatgc gcggcctcag ccttggctca tggcagatcg
     5941 cttctaccgc attcgacaat gtgctcggct ggtccggacg cgaaggggcg gagatcgtgg
     6001 cgattgccca agtcgctcag gggattacct tgccggtcta tgtccggccg gagatccaaa
     6061 cctgggaaga cttgcgcggc aaaccgcttg ccgtggacgc ggtcgacaca gcgtatgcat
     6121 tggttctccg gcgtatcttg ctggcccacg gacttgaaat ggagcgcggc gattatacct
     6181 tgctacccaa gggcgctacg gggcaccgtc tggaatcgat gcaacaagga gaaagttttg
     6241 ccggagtttt aaatccgccg tgggatgcga aggcgaacgc ggcaggcatg aagcgcttcg
     6301 gcgatcagcg cggaatcctg cccgactatc ctggcggtgt tttcgcggtt aaccgtaatt
     6361 gggccaatga taatcgcgcc atcctgatca agtatctggc tgcttgggat cagggtctgc
     6421 actgggcgca ggatgtgaaa aatcgtgacg aggcgatcaa gctgcttgcg caagccgaga
     6481 agatggacga agcaagttcg gccaatcgtt tgcgccagct cccgagcaac ggccaattga
     6541 acttggcggg ccttcaaacg gttctggacc tgcgcgtgca attcggactg actccatcca
     6601 tgggtaaaga tttgctcaag tattatgacg agagttttta taaccagacg gctaaataac
     6661 aaagcgcttc gcaaatgtta gagcaaaagc caaggaggat cgaatgaaaa agttatggac
     6721 tcgttttgcc ggcctgtggc tcggcttgat ggtcgcagct tggacaaatg ctgccgtcgc
     6781 ggctccagta gacgatttga tcgcaggcgc caagaaggag ggcgtcatcg atttctatgg
     6841 tccttcaacg ttggggcctg agggcgctca agcgatcgtt gctgctttca acaagaagca
     6901 cggattgaat atcaaagtga attttacccc gtccggtaac atgacccggg ataccgcaaa
     6961 gatcgtcggc ctatcggcct cgggacaacc gccggaatgg gacatcatgg tcgtgaccga
     7021 cgcccatcac ggctctcttt ggctgagaaa attgcataaa ccttttgact acgccagcct
     7081 cggtgtagcc aaagagcggg ttgagtatga caacggcaca gtctccgtcg cgaaccaatt
     7141 tgccctgccg gcttacaaca ccaaattact tccagccaaa gatgttccaa aaacttggga
     7201 agatctcctc gatcccaaat ggaaaggcaa aattggcgtc attaactcga cgcaccattg
     7261 ggggcgcttg gccgcaggcg cttggggcga cgagaagaca ctggatttca tcaaaaagct
     7321 gtccgcccaa aagccgcttt tgacccgcgc gggtgagatg gcgcagcgct tgatcttggg
     7381 tgaagtgctg gtgtcggcaa ctttgcagga tagccaactg cacgagtcga aagaatccgg
     7441 cgcccccttg gcttttgccg aacaggtttc gccggttatc tcgcccgagt atcacgtcgg
     7501 cgtgctgaag aacgcagcgc acccgaatgt cggccatctg ttcgtaacct tcatggcctc
     7561 tgccgatgtg caaccggtct ggcagaaata caccggccat acttccgcgt ttgttccggg
     7621 aaccaacgct tacaagttcg cgcagggcaa gaaggtcctt tacatgaaac aggaccaagc
     7681 cgagaaggtt gacaaaatat cgcgacaaat cggcaagatt ctcgggtttg attaatcccg
     7741 gcgattacgc gaaagcgatt gggagaggtc atgaaagcag aatctcggac tgtcgtgagc
     7801 tccaacgcgt gggaggggct cagccgtttc ttgccccagg gtgtcacgtt gttcgccacc
     7861 ggcctttccg catttttgct tgttctgatc gggttgccgg tcgcgatggt cattcttatg
     7921 agccttcgta ccggcttccc gggcgaacag gtgccgttca ccttggaaaa tttcgccacg
     7981 gtatatctgg cgccgagaac ctacgagatc ttgctcaata cggttttttt tgcgatttcg
     8041 agtgtgtcta tcacgcttct gatcgccgtc ccattggtct ggatccttat gaggaccgac
     8101 atccctttca aaaaaaccat ttatgttttg ttgactattg gaattctcat cccggtcttt
     8161 ctgcgcacca tcgcgtggat cttgctgctt agtccccgca ttggactgat caataaatgg
     8221 ttgcaacaag cttttgggct gatggagccg ccgttcaatt tgtacagtct taccggcatg
     8281 gccttcgtgc aaggcgtgtc tttcgtgccg ggcgcgtttt tcatgctggc ggcggcttac
     8341 cggtccatgg atccctcctt ggaagaggcg gcctatacct caggcgtcgg caagctcagg
     8401 acttttttga agatcaatat tccgattacc tggcccgcga tcgctgccgt gatggtttat
     8461 ctgattatga ccgccgtcgc ggttttcgag gtgccggcaa tcatcgggct gccggcgcgc
     8521 attcacgttc tgagctcgct catttttact tcgacaacgc cttcaaccgg tctaccgaac
     8581 tacggcatgg caggcgcata cggcgcaatc atgctggttc tcggactcac gctcgctttc
     8641 ttgtacgtgc gattggtcaa gcaagggaaa aaatacacgg tgatcaccgg cagaggatat
     8701 cgcccccgag agatcgcgct cggccgatgg aagtgggcag cgctcgcctt tgttttcttt
     8761 tatctgtcga tcgaggtttt tgtcccattc agtgttttgc tgtgggcctc actattgcct
     8821 tatttacagc taccctcggc ggaagctttg tcgtcattga ccctgaaaca ttatatcgag
     8881 ttgccgactc acgtcggtcc gcggccgttc atcaacacgc tgattcttat gtttgccgtg
     8941 ccgacaatca cgatgttttt aagcgtactg gtctcttgga tagtgattag aactcgagtt
     9001 tccttccgcg gtttcttgga taccctggtt tttctgcccc atgctgttcc agggatactc
     9061 atggcggtgg gactggctta tctcggactg gcctatcgcg attatttccc cctctacggc
     9121 actgtctcga ttatcgtgct cgcgcatacc atcaactgga tcgcctatgg cacgcgcacc
     9181 accaacagcg tcatgattca agtgcaccgt gaacttgagg aggcgggccg ggtttcaggg
     9241 gcttctgcgc cccgggtttt aggaaaaatt attttaccgc tgatcgccgc gggcgtgctc
     9301 aatagctgga tctggatcgg catgctctcg taccgcgagg tcaccatgtc cttgacgctt
     9361 ctgacccgta gcaatgtcgt aatctctacg gtcgtctggc agttctgggg cagcggttgg
     9421 gttcctcaag tctcggcttt gggagtgatt ttgattttgt tcgcaaccat cgttgtcggg
     9481 acggtgcgct ttgcgctttc ccgtattggt gagatcggcg cagcatccta gcagcctgct
     9541 aaaaaaaccc atatgcttcg ttgccgctca atcgcctcgc ttcaaccgtt ttttgatcgc
     9601 tgcgtcggtg agaaaagatg attagcattc gtcaattgca aaagtccttt caggctcaac
     9661 aaggacgggt cgacgcttta aaaagcatcg atttggaggt gagcgaggga gaattttgtg
     9721 ttttgctggg cccgagcggc tgcggcaaga caaccaccct cagatgcgtc gccggactgg
     9781 agaggccgga tggcggggaa atcgaaatca gcggccggct tgtcaactca ccgagatcgc
     9841 gaatttttgt tcctgccgag aaacggcaaa tcggcatggt ttttcaatct tatgccattt
     9901 ggccgcatat gaacgtattt caaaacgttg ccttcccact cactcaaggg caaaagcggg
     9961 tctcgaaatc ctcggtagcc gagagagtgc gcgccgcgct caaacgtgtt cagctcgacg
    10021 gtctggaaga tcgacctgcc accgatctta gcggcggcca gcagcaacgc gttgcgatgg
    10081 cacgcgcgat ggtaaccgat tccaaagtcc tgttgatgga cgaacccctc agcaatctgg
    10141 acgcccggtt gcgtgagcag atgcgcgtcg aattgcgcaa gatcacgaaa tcgatcggcg
    10201 tgacaactct ctacgtgacc catgatcaag ccgaagcttt gagcctaggc gataaagtct
    10261 gcgtcatgaa ccacggcgaa atcctgcaga tcgcccagcc caacgaagtt tatgcccgcc
    10321 cggtgaatct gttcgtcgct caatttgtcg gcgaaatgaa cttcgtcaaa gcagaagtca
    10381 ttgacgcggg agaggttggc tgtccattgg gtagactgcc cctgtcaatc cctaacgggg
    10441 tgacaaaggg cgccaacgtc actctggcaa tccgccccga gcatgtaaag gtgttgccgg
    10501 cgacccacag tgccgccaat cgcatgcccg ggaaaatcac cagcaaaaac tatctgggtg
    10561 attcggcgct gctggaagtg gaagtcaacg gcacgacact gctcgcaaaa ctcgatggcg
    10621 acaccgagtt gtccatgggt gacccggctg cggtggaatt cccggcgcac cgctggcatg
    10681 tgttcccgca gaattaattc accgttcaac ggttcgaaag ttcaacgccg aactagaatc
    10741 agctcaaagc gttcagcaaa ctccaggcgc gggcgcttca acattaggtt aaaatctttg
    10801 gattatcggg tcccttagtc acgtgagact tgacgttgaa cgtttgaacc gtggaactgt
    10861 cgaactgccc ggttcatttc ttatagagac tcttgataaa tcctgagtct tctagctctt
    10921 tgacaaatcg tgtgtccacc agatctttag cattggcttg cgccgccttc ggattggttg
    10981 cggccatatc ctgtaggatc aattgatatc cttccaccgg cgcgtacggc gcttcgggga
    11041 tgaacttagc atgccagtcc caagcttcct ctaaggtctt tcgatcttcc aatcgagaat
    11101 atttgcccat gatcttgatg gtgtcttctt tgcgggtttt gaaaaaatgg atcgcttcgg
    11161 tgtatgcccg catgtaacgc cggaccatat ccgggttctc acgaatcagt ttcgtcgagg
    11221 tatagacacc gttttgcgga aacggtattt gcagcgccgc catatcgacc acagtcggat
    11281 agcccagctt ctcggcgatg gctttacccg cctcggtgac gaccgtgaat tgcacgatgc
    11341 ctttctgcag agcgaccatc cgatcgatgg caccgccgat tacccgcagg ctgacatctt
    11401 tggcagggtc caagccgatt ttttttagac ctactttgag agcgaactca gcggtggtcc
    11461 cagctatatg tgtgccgccg atttttcccc tcaagtcttc ggcttttttg atgctcggtt
    11521 gtgataccaa atagtaaggc aagacattga cattaccggc tatggcaacg acatcaccgc
    11581 cggccaagcg cgcgctcgcc agtcccggac cgccgccttc gccgatggga atttcgccgg
    11641 cgaggatcgc ctggatacca cggacgctgc ctgagataaa aatcaccgtc gcatcgaagc
    11701 catgcttgtc aaatatcttg gcgtccttgg cgatccaaat gaccgaagat gaacccggag
    11761 tcgtagagat gtacgaagta ttgatcttct gggcggcgac caacgaagga agcgcgtaga
    11821 agatcacggc gacccagagc aatgttttgg ccggacgtat acgtttcatt ttacctccgc
    11881 agcattctat aggacgaact gtctgagagt gatatcatct tcgaagagtg gtgaaaagtt
    11941 gaattgacag cgggcggacg gcggattggt ttgcgagaga agcaacttcg atgaagcgat
    12001 cggttgattt gatcatgttc gatcttgatg gaacactggc cgacacagga ccggatcttg
    12061 ccgactcggt taactatacc cgctcgcgtt ttgaacttag tccattgccg cagcagttga
    12121 tctatactca tgtcggccgc ggcgtcgagc accttctgcg acatgcggtc gcgaaagagg
    12181 caccctccca gttctccgag attatgcgcg tgtttctgga acactatcag caacatctgc
    12241 tcgacaaaac cacgctgtat cctcacgtcc atgaggtgct ggattatttc gatggcaaac
    12301 gcaacgccgt ggtgaccaac aaattgcacc acctggccgt aaccgtgctg cgcgggctag
    12361 gtatcgaaac ccgatttgac gcagtcttgg gcggtgacag cggcaccgaa aaaaaaccgg
    12421 atccagcgct gctgcgcttg gtgttaagac agtttcaaat tatcccagcc aaagcgttga
    12481 tggtcggcga tggtgacacc gatatccaag caggaaaatg ggccggcgtg atcacctgcg
    12541 gtgtaactta tggactagcg aacaaggacg atttggttgc cgccaaacct gactttctga
    12601 tcgacgatct cgagcaattg acgcaatatt tttgttaaca aaggacccga taaggagaca
    12661 gatcatggcc gataaaatac ccgaaccatt caaggatcta tttgcgaaag ttgcctatgc
    12721 aaatcttgcc accgttatgc ctgacggcag cccacaggta acaccggtat ggttcgatta
    12781 tgacggtgac tgcctgcgga tcaattcagc caaaggccgg atcaaagaca aaaacatgcg
    12841 gcgcgacaag agggtggcct tgtcgattca agatcccgac aatgcctatc gttatctagc
    12901 ggtgcgcggc gccgtggagg aaatcaccga gcagggcgcc gacgctcaca tcgattccct
    12961 tgccaagaaa tatttgggca aggacaagta tccttttcgc ggccccggcg aagtccgggt
    13021 catctataaa atccgccccg agaaaatttc caccatgagt taatccagcg acccgctgga
    13081 ggccggatat ttcaggctag atggcaggcg cgcaagcgat tgccggaaag cgattcaagg
    13141 cagcgagagc ggctacttta tagcattccg cgagtgtcgg ataattaaac acggcgtcga
    13201 tgaagtaatc gagcttgccg ccgagcgcca tcacagcctg cccgatgtgg accaattcag
    13261 ttgcgccctc gccgatgatg tgaacgccga ggagcgcgcg ggtgtcgcta tgaaacaaca
    13321 gtttcagcat gccgacggtg tcgccgatca actgacctcg ggcgatctcg cgataacgcg
    13381 caacaccgat ctcatagggc acgccatcac gggttaactc atcttcattg cgcccgacca
    13441 tagaaatctc cgggatcgtg taaattccgt acggcaatag gtgagtcgag gtgtcgcaag
    13501 caagacccaa ggcatgacag gcggcgtgcc ggccctgttg catcgaggtt gaagccaacg
    13561 ccggaaatcc aatcacatcg ccaaccgcat agatatgcgg cagcgtcgtc tgatagtttt
    13621 cattgacctt aaggcgtccc cgctcatccg gttgaacgcc gatactttct aaattcactc
    13681 gcttggtagc gcctacccgg cccaacgaat acatcaccaa accggcgcga atttgtttgc
    13741 cgctcttgag ctgaattacc accgcgccgt cggcatgctt ttgcacggcg gtaacttctt
    13801 cgttgaagcg aagcgtaatg ccaatgctac gcatttggta ttgaagcgct tcgatcaatt
    13861 cactatcgac aaattcaagc aagcgggggc gccgctcaat caatactacc ggaatcccca
    13921 gcgtcgcgag aatcgaagcg tattcacaac cgatgacgcc gccgcctaca atcgtgagcg
    13981 atttcggcaa ctggttcaga gccagcaacc cgtcactgtc gatgatcgtg gcatcatcga
    14041 acggaatccc tgccgggcgg gacggctcgg tgcccaccgc gatcacgata aagtccgcgg
    14101 tatgctcatc gatctcggaa tcctgtgtga ccgagagtgt atggggttca atgaagctgg
    14161 cgtggccgac gatgaaatca acgtgattac gaatcagctg attttgcacc acctcctgct
    14221 ccgctttgac gacatggtcg cacttaaaaa gcaggtcctc gaaggtgatg tcttttttga
    14281 ctcggtagct ggcgccgtat aaatttcttt gccgaaaacc tgaaagaaac aggacggctt
    14341 cgcgcaaaga tttgctcggg atggtgccgg aattgatgca cagtccgccg acgaattttt
    14401 tttgttcgac aatgccgact ttcttgccga cctttgcccc ttggatggca gctttttga
//
LOCUS       DASUVS010000124        28419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1574385, whole genome shotgun sequence.
ACCESSION   DASUVS010000124 DASUVS010000000
VERSION     DASUVS010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 28419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28419
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574385"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VF182_07000"
     CDS             complement(<1..677)
                     /locus_tag="VF182_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6436860.1"
                     /translation="MVLVLKNEQMEDLLPMAEEIDAIEEAFRELGAGQAMNAPRARLR
                     TPWKEEGGQYYFNNIMGLVPGVKSMALRIDSSFSKEVQIDGAKRRIYPGDFIGLVMLF
                     DMDTCELLAIMDDHHISAMRVAATSAVASKYLAREDSKIMGLLGSGEQAKTQVTAHLA
                     IRPINKVKIYSPTKENREKFAREMSASTGIEVLPVTSAEEAVRGSDIVSAATNTVDPV
                     VQGRWLE"
     gene            complement(993..1265)
                     /locus_tag="VF182_07005"
     CDS             complement(993..1265)
                     /locus_tag="VF182_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX6436861.1"
                     /translation="MTYVIAEPCIDTKDTACVDVCPVDCIHPTKNETEEFEKENKLYI
                     DPEECIDCGACEPVCPVEAIFEEAAVPDKWKHFIKIDADWFKAKKG"
     gene            1539..1940
                     /locus_tag="VF182_07010"
     CDS             1539..1940
                     /locus_tag="VF182_07010"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6436862.1"
                     /translation="MAKLRHLAILTHQPEKLADFYKKVFELKEMYRTKNGSVHLSDGE
                     VNLAILNATDPKEPGHAAGMYHFGFHVDDAEVVAQRIQELHPEGAPKARPKGTSYAET
                     RGSDPDGNLFDISTWGWGEKLLPKEKGGPGA"
     gene            2122..2571
                     /gene="ybeY"
                     /locus_tag="VF182_07015"
     CDS             2122..2571
                     /gene="ybeY"
                     /locus_tag="VF182_07015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX6436863.1"
                     /translation="MVIEFARRGAGAGYPTADLRKIAASILTALEQSRAELSLALVGD
                     REMRPLNAKYRKKNKTTDVLSFPADPAMCSEAGLLGDVIISIEQAKRQAKERKTSLKI
                     EMATLLIHGILHLLGYDHERSERQAKIMASLERKLLRHLCERGLIKL"
     gene            2809..3919
                     /gene="prfB"
                     /locus_tag="VF182_07020"
     CDS             join(2809..2865,2867..3919)
                     /gene="prfB"
                     /locus_tag="VF182_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_123290836.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX6436864.1"
                     /translation="MLEETKEQLVQLEQRLDKLGGVFDVESKQKRLEELTQITSQPDF
                     WNDADKAQTIFKEQSSLKAVIDNWEKHRTGLEEARFFLDIAGEEESDEALQEAAAKVS
                     EVTQGMAETELAQILGGADDRRNAIVTLHPGAGGTEAQDWAEILLRMYLRWSDRHGYR
                     KEILEYQPGEEAGLKSVTFSVEGDYAYGYLKAEAGIHRLVRISPFDANSRRHTSFASV
                     FVYPEVDDTIKVEVDEADLRVDTYRSSGAGGQHVNKTDSAVRLTHLPTGIVVACQNER
                     SQHKNRAMAMKILRSRLYELELEKQKEKMDTLHKTKKDNAWGSQIRSYVLHPYRLVKD
                     HRTNIEIGNADAVLDGDIDSFIQAYLLHDSDSPAA"
     gene            4076..5278
                     /locus_tag="VF182_07025"
     CDS             4076..5278
                     /locus_tag="VF182_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006619316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6436865.1"
                     /translation="MGGENREARIGSDDGGVQNIEAAVLERYGDAALQVEACLCTPVS
                     YDRTLLDIIPGEINEKDYGCGDPSRHVRAGETVLDLGSGSGKACYIMARIVGAEGKVI
                     GVDFNPPMLQLARKYQESIGDKLGYHNVEFRRGKIQDLRTNLELIDLFLQENPVRSVA
                     DLARLESFQNTVRDEQPLIADESIDVIVSNCVLNLVRPEDKKLLFAEMYRVLKRGGRV
                     VISDIVSDEPVPEHLSKDPDLWSACISGAFQEEDFLRGFEEANFYGIQIEELSSEPYQ
                     TVEGIEFRAVTVTAYKGKEGPCVERNQAVVYRGPWKQVVDDDGHTLERGARIAVCDKT
                     FKLYSQPPYQGQFILLPPRQEVSLDKAGLFDCSRDHKRHPRETKGVGYKVTSAPTSAC
                     TPGSTCCP"
     gene            5383..6336
                     /gene="arsS"
                     /locus_tag="VF182_07030"
     CDS             5383..6336
                     /gene="arsS"
                     /locus_tag="VF182_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551656.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HEX6436866.1"
                     /translation="MQPFSYKIHPTNGLTRRAIDTLQVNLGRYCNLACIHCHVESGPT
                     RTEMMNRETVDAVLRFLASSNISDLDITGGAPELNPNFDYLVESAVGLGRHVMDRCNL
                     TVIFEPGKDYLPEFFGRYGVELVCSLPCYSEENVDRQRGKGTFDLSIRALQILNELGY
                     GRDEHLVLNLVYNPVAPHLPPPQQHLEQDYKTILKQQFGIVFNQLYCLANMPITRYET
                     HLKLRGEYNGYMELLESSFNRATLDRVMCRNLISVGWQGLIYDCDFNQMLELPMRDAA
                     GKALEIASLKVEHLLNRAITVGNHCYACTAGSGSSCGGALS"
     gene            6508..8763
                     /locus_tag="VF182_07035"
     CDS             6508..8763
                     /locus_tag="VF182_07035"
                     /inference="COORDINATES: protein motif:HMM:NF004991.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEX6436867.1"
                     /translation="MKPLKILLIILLLIAVGIALVSFPLRHWFMQLESYVESLGPVGP
                     MVVVLAYVFLTVLLVPGSAITVGAGTLFGLKIGFIVVVLGANLGALVAFFLARTLLRQ
                     RVARWAESNPRFRLLDRAIGKQGFKMVLLTRLSPIFPFVLLNYFLGLTAVRTGAYVLG
                     NLLGMLPATFLFVYLGAAARDALAGQLNVSVGLYQQILKVVGLLATIAVVIMITRIAR
                     QALRQTEQLDEGSTVPGKPRLEPNYRPATFDKMMIVDDAHDRRLIQNCHPPHWINPAP
                     AGKYNLVVIGAGTAGLVSAAGAAGLGAKVALIEHNLMGGDCLNIGCVPSKAMIRAARA
                     AHDARDAARFGVEIACEPQVAFAGAMERMRKLRAGISDHDSAERFRKLGVDVFIGTAR
                     FTGASSIEVDGKRLEFRRAVIAAGARAAKLPIPGLEKTGYLTNETVFTLTELPRRIAI
                     IGAGPVGCELAQTFARFGSKVTLLSDGAEILPKEDADAAAIVRKKMEQDGVRIVTKAK
                     IHRAEKNNGEKILAATVGEAAVDINCDAVLVAAGRTPNLEGLDLEAAGIGYTYRGVEV
                     DDWLRTKNPRVYAAGDVCSQFKFTHAADATARLVIANALFFARRKVSALTIPWCTYTD
                     PEIAHVGYYEKDARQAGFAVATLTESMKDVDRAILDGEEEGFARVHYDQKSGKILGGA
                     IVARHAGEMIGELTLAMVAKQKVGVLSSTIHPYPTQAEVLHKIGDAYMRARLTPRVKK
                     TLQTWLRWQRV"
     gene            8801..9484
                     /locus_tag="VF182_07040"
     CDS             8801..9484
                     /locus_tag="VF182_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014291852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX6436868.1"
                     /translation="MPVSVSVIIPALNEENALPTILADLIALQPHEVIVVDGGSRDRT
                     REISEQQGARVITGTAGRARQMNRGARQATGEILLFLHADTRLPRGAFAEIAAALKNP
                     GCVGGRFDVDLDGNHWLLKIIGAMINRRSRITKVGTGDQAIFVRREVFVRMGGFPDIP
                     LMEDIAFCRALKRLGGIACLRSKVRTSARRWEAEGVWRTVMKMWILKLLYFAGVSPVT
                     LKRFYGDAR"
     gene            9635..10327
                     /locus_tag="VF182_07045"
     CDS             9635..10327
                     /locus_tag="VF182_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX6436869.1"
                     /translation="MSLSRDSANALVILTKAPQPGESKTRLVPPLSYAEAADLARALL
                     LDQLQNLATFDAARLFIAFTPENAAVFFEDFIDQGFTCFAQQGQLLGQRMSHAFQYLF
                     ASGFENIILIGSDLPTLPVGFFHQGYASLDKSADVVLGPSADGGYYLVGLKRMIAAIF
                     DDIGWSGADVLSQTIHKLDDLGVKYELLSEWYDIDTVKDLERLLSQRRCHEVAMKNTF
                     ALLNELTRRGRL"
     gene            10440..12488
                     /gene="tkt"
                     /locus_tag="VF182_07050"
     CDS             10440..12488
                     /gene="tkt"
                     /locus_tag="VF182_07050"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX6436870.1"
                     /translation="MQNKTDALCVNTIRMLSVDAVEKAKSGHPGAPMGLAPVAYVLWT
                     RFLKHNPKNPSWPDRDRFVLSAGHGSMLLYSMLHLTGYDLPLDQIKQFRQWGSLTPGH
                     PERELTPGVEVTTGPLGQGFGNGVGMAIAEAYLAARYNRPGFEIINHFTYAIVSDGDI
                     MEGVAAEAASMAGHLKLGKLIYLYDDNRISLAASTDLSFSEDVGKRFDAYGWHTQSVA
                     DGNDLNAIEHALDRARSETTRPSLILVRTHIGFGSPGKQDSFEAHGSPLGAEETKLTK
                     QNFGWPPEPFHLPEEALAHFRESIAKGQKIEAQWNEQCTAYQREFPELGRELEQALRG
                     ELPAGWDAAIPDFPADAKGLATRAASGKILNAIAPKLPTLIGGSADLNPSTFTALNNL
                     GDFENPQGDFSKSQGLTGGGCNYAGRNLHFGVREHGMGAAVNGMAAHGGIIPFGSTFL
                     IFSDYLRPSMRLAALMELGAIYVFTHDSIGVGEDGPTHQPIEQLAALRAIPRLVLIRP
                     GDANETAVAWSLAIETRNRPVALALSRQNVPTLDRTKYTAAAGVRRGAYVLADPPEGK
                     PDLVLIGTGSELSLVVAAREKLLQQKINARVVSMPSWEIFDDQPEDYREFVLPPDTRA
                     RLAVEAALPLGWHRYVGDRGDVLAIERFGASAPGNIVMEKFGFTVDHVVERARALLGK
                     "
     gene            12491..12946
                     /gene="rpiB"
                     /locus_tag="VF182_07055"
     CDS             12491..12946
                     /gene="rpiB"
                     /locus_tag="VF182_07055"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX6436871.1"
                     /translation="MRVAIGADHAGFQLKQVISEHLRSAGHTIIDVGTASDAAVDYPD
                     YAEALAKVVLDGKAKRGVLICGSGVGASVAANKIPGVRAGLCHDSYSARQGVEHDDIN
                     VLVLGARVIGSALAKELVDRFLAAQFSREERHLRRVGKIKALEQRYQKS"
     gene            12958..15717
                     /locus_tag="VF182_07060"
     CDS             12958..15717
                     /locus_tag="VF182_07060"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEX6436872.1"
                     /translation="MNPLRELLNQGQSVWLDFISRQLMRSGELKRLVEEDGLRGVTSN
                     PTIFEKAIGGSADYDESLREMLAQDRKTPVGRLYERIAIEDIQAAADVLRPVYDQTSG
                     ADGYVSLEVSPHLARDTQATIAEAKRLNTSVDRPNVMIKVPATAEGIPAIEELIAGGV
                     NVNITLMFSLAHYEAVAHAYIKGLERCADPAKVASVASFFVSRVDTMIDRELEKLGTP
                     EAKSCLGKIAIANSKVVYQRFREIFHGEGFVALRQRGARVQRPLWASTGTKNSNYSDV
                     LYVENLIGPETVNTLPVETLNAFKDHGRVSDATVRQGLDEAAKALTQLKEVGIDLDAI
                     AEKLQDDGVDAFATSFDSLMSTLEKKRSTLAGIELNRQDLHLGKLQSRVDRRLKAWQA
                     EQFGSRLWKKDPTLWSADSAPELTDRLGWLEMPQSMQQQVEALQRFAEKAKADGMRHV
                     VVLGMGGSSLAPEVFQQSFGNQSGYPALMVLDSTHPAAIRSLEGKIDLARTLFLVSSK
                     SGTTTETNSFFYYFWDKLKQLKAEPGDHFVAITDPGTALEKLAGERKFRTVFTAPEDI
                     GGRYSALTVFGLVPAALIGLDVEDILKRARHMSEACGESVPEGKNPGLALGAALAELT
                     LAKRDKITFLCSPSLRAFPAWIEQLIAESTGKDRKGIVPVAGEKLGAPEKYGADRAFV
                     YLRYESDENHELDRQVAGLEANGHPVLRIEMLEKTDLGQEFFRWEIAIAAAGAALGIH
                     PFNQPDVQLAKDLATKAMRESRAGNRRGAKLKEEVSSADDAAMQQAASSWLSKAKPRD
                     YVSIQAYISPTPEHDVALQTISEALRDRLGTAVTVGYGPRFLHSTGQLHKGGPNTVLV
                     LQIVDHPSEDVPVPETDYTFGALIAAQALGDFTALKQRRRRALRINLGDDTKAGLERL
                     VVALR"
     gene            15904..16800
                     /gene="gnd"
                     /locus_tag="VF182_07065"
     CDS             15904..16800
                     /gene="gnd"
                     /locus_tag="VF182_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX6436873.1"
                     /translation="MELGMIGLGRMGANMTERLLRGGHKLIIYDRSPEAIQRCVDKGA
                     VGTHSLADFVKQLSLPRVIWLMVPSGDPVDLTIEQLIPSLSKADIIIDGGNSHYKDSI
                     RRAEKLKQLGIHFVDAGTSGGIWGLQEGYCLMIGGEKAIVDRVDPIFKTLAPENGFCH
                     VGPSGAGHFVKMVHNGIEYGMLQAYGEGFEMLKASQFDLDLGQISRLWNQGSVVRSWL
                     LELAENAFQKDPQLSSIKGYVEDSGEGRWAVLEAVEREIPASILTLSLFARYTSRQED
                     SFSAKVIAALRNEFGGHAVKKE"
     gene            16806..18374
                     /gene="zwf"
                     /locus_tag="VF182_07070"
     CDS             16806..18374
                     /gene="zwf"
                     /locus_tag="VF182_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX6436874.1"
                     /translation="MASDPQDYVPSGFSQAKKPHSCVLVIFGASGDLTKRKLIPALYN
                     LALEKRLPERFSVVGFARSNMSDGEFRNKMREAVSEFSRTGLKDEQVWQQFASTLYYV
                     RGDYNESGAYQRLKSMIDGFDRGSRVLPVRVFYLAMPPDLYAPVIERMAAVGLAPKES
                     EGTARTRVVIEKPFGSDLESARELNRRVHEALDEKQVYRIDHYLGKETVQNIMVLRFA
                     NAVFEPIWNRRYVDHVQITAAETVGVETRGGYYDKAGVARDMFQNHLLQLACLTAMEP
                     PTGFSADAVRDEKSKLLRAVRPITDDQVALHAVRGQYGTGRINGKPVPGYREESDVAR
                     DSSMPTYAAMKLLIDNWRWEGVPFYLRSGKRLFRRVTEIAIQFKRPPLLLFKSCAVED
                     VAPNVLVIRIQPDEGVALTFEVKPPGPDICVSPLKLDFKYEEAFGNTSPEAYETLLED
                     CIEGDSTLYTRHDWVELAWLLMDPVIKTWERIQPKDFPNYEAGGWGPKDADEFMQRDG
                     RRWRRPLNNTNQPG"
     gene            18486..19502
                     /gene="glk"
                     /locus_tag="VF182_07075"
     CDS             18486..19502
                     /gene="glk"
                     /locus_tag="VF182_07075"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX6436875.1"
                     /translation="MSPFQQPDRELILAGDIGGTKTNLALFSYQNNNLKAEFQKTYSS
                     QEYSGLVPILEEFFATGKHPISRASFGIAGPLVDGKVNPPNLDWVVDPVSVAKTLELP
                     SIALLNDLEAAAYGIFTLTPDEFLVLNEGIVRRPGNKVLIAAGTGLGETILFDDGKDY
                     YPLASEGGHADFAARNELEIELLRYLIGRFGRVSYERVVSGPGLANIYDFLKEKVGLS
                     VPGPFADKIAAAEDRSPLISQAALAGAPEIAVKALDMFVSVYGAEAGNLALKGKAIGG
                     VYVGGGIAPKMLDKLKDGTFMQALNDKGRYRELVSSIPVYVVLNEKAALRGAAYHATL
                     GRSG"
     gene            19511..20350
                     /gene="pgl"
                     /locus_tag="VF182_07080"
     CDS             19511..20350
                     /gene="pgl"
                     /locus_tag="VF182_07080"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX6436876.1"
                     /translation="MAERDIVLCRDLVELSHKSAEQFSQLARESIEASGRLTVAFSGG
                     STPKHLYSLLASPAYKNRIQWNNVELFWGDERCVPPDHTESNFRMAQEALLSNIEIPA
                     ENIHRMRGERQPQAAAAEYEKELQKFFGLDSGGLPRFDLILLGIGEDGHTASLFPGSD
                     ALAPSFILPRLTGEERGGGVAHDRPSQSHRGHSETETVHLVVAPFVEKLKSFRLTLTL
                     PVLNNAADVWFLVAGSSKAGAVKQSFSSSSDLPAAKVQPVNGKLTWFITRDAAPGIPA
                     AGK"
     gene            20497..21495
                     /locus_tag="VF182_07085"
     CDS             20497..21495
                     /locus_tag="VF182_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6436877.1"
                     /translation="MSGKLLVVLTFILCAVSRPQAQTLDEVYKRALTEGGTLNFYATL
                     AQVNAASILPGFEKRFPGIKVNHVDATSDQLASRVIAESRGGKVVADVFQTLLDGMIR
                     VQSQGLFLEKLYPEAAEYPVELKGTTWVATDLIYIVAAWNTTKIKKQDEPKQFDDFAD
                     PRWKNQLIAEPRDFQLLLGLAKHKYKSDEKAVALLKRIADNNVQFHKGHSQLAELLVA
                     GQAAACVTCYSHHYPSRKRKGAPVDYLLTEGTGDIDATAIMKNAPHPNTALLFARWTA
                     SADGQKIYAQGGRLPAHPNVEAVEKTRPTKVYPLGEQDVKDYPKYEKMWKEIFGLR"
     gene            21579..21749
                     /locus_tag="VF182_07090"
     CDS             21579..21749
                     /locus_tag="VF182_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436878.1"
                     /translation="MARTMIHNDSMEIQNIPCTVCHECDYEQIGKQVQKKIDKLLERA
                     ARGKLKSRLVVM"
     gene            complement(21763..23133)
                     /locus_tag="VF182_07095"
     CDS             complement(21763..23133)
                     /locus_tag="VF182_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX6436879.1"
                     /translation="MAQVIYTGLIIILAAFHLAFVSGCAIIQYGTHANEGPVLTSPAP
                     TRATTKISSSDLKNGTFIGIGMSGGGSRAANFSAAALLQLESLGILQQATALSSVSGS
                     SLTAAYYGLFSDDNIHHWNAQQVRERLVKNFELEWFWRWFLPWNIGRYWFTGFDRSDI
                     MADVFDHNLFEDKTFGQMPRTTPKILINATSLTDNGASYLFTDEAFNKIGSGLDKYAV
                     SRAVMASGAFPGAFHNVTLRDYTQPKQYVHLFDGGPSDNLGINSLRKVARDLYLGPES
                     ERPRKCLVLIVDAYTQGWNLSREDPDTRKAITDFVIDTNALEASSVLLALRRDRLLEE
                     MGISSDGNRAPDVYEPFHLFAEADDPELKKDPKLKDVECHAWLITFQRLQHLTHLPNA
                     GTISAIVNNIPTRYTLLAPHGLGAAEAQEALFNAAEMLIKEDKETLAAICKDFGDNFA
                     EMKCPK"
     gene            complement(23428..24564)
                     /locus_tag="VF182_07100"
     CDS             complement(23428..24564)
                     /locus_tag="VF182_07100"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6436880.1"
                     /translation="MVTELTFLTLILSKPRHGQSNKLKRLLLSLLTGAIVLSAGAAVL
                     APVTSPALAAESPAPTSRHIQWLGTSSWILSSGDDVVVVDPFFTRPSFARVSASLMMP
                     YLTSNFTYDPERIRAVLPELPGNTRFVLLSHGHYDHLLDVPYYIIRNSGRSVTYIGSL
                     TARNILLAFKPPALDFVIAEQKPSIIKGRIRVTALASDHAPHLFGHTFMAGDVVSPMT
                     SIPSRVGQYLEGQTLIFLVDFLDDQESVLWRIFINGAANSAAGAEALRKYQSLLNEHR
                     VNIAILCVPGWDKVDDYPDTLLRLLRPENIVLSHYDDFGSPYISGEDPNNGMRALPFA
                     NYDGFVERLRLLNTSNNYGFSIYQPKTGQCISFSDSNPTTSCQQ"
     gene            complement(24592..25446)
                     /locus_tag="VF182_07105"
     CDS             complement(24592..25446)
                     /locus_tag="VF182_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6436881.1"
                     /translation="MMNETIPATELDDPLLPVPIPEQAPAKDGMAELPGTHLGYWDTG
                     GAGEPVVFLHPASGSALIWLYQQPVFSGAGYRVIAYSRRNYHNSDPAPDDNPGSGSED
                     LDNFIEYLGLKKFHAVSSAAGGSVAADYALSHPERLLSLTISSNNLAAADGYIAEAAA
                     RIKLPEWDQLPRWFRELGPSYRAANPGGVTKWIELNHKSELGRGARQKLCNVVTPEKL
                     QTLKVPTLLMTGAADMFTPPAITRMIARHVPDNEVVIAAECGHSIYWERPDFFNRTVL
                     GFIGRHSE"
     gene            25679..26218
                     /locus_tag="VF182_07110"
     CDS             25679..26218
                     /locus_tag="VF182_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436882.1"
                     /translation="MNPMFIVAIPIVLIVIGMVLNSTGLSFDQPPSSSEQDPAKKSAA
                     DADAYRRFFDVQRKQAVKRQARVGQYAWLLMAAIIGSFIWLYMYTVSKTSLATQIGAV
                     QTLATEDGKEAVLSITLKDGSNVKYLIKPATAEIIEASKKEGNSKEKISSYEMSQLGT
                     AFNVGESPLSPGIAIKLSK"
     gene            complement(26397..27953)
                     /locus_tag="VF182_07115"
     CDS             complement(26397..27953)
                     /locus_tag="VF182_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436883.1"
                     /translation="MKRLLSFVVLFLPFTVIALLALNISLALAIGDTPEAKNMRLVGF
                     NNLQARSAYQPIIHKQGNRWIAYIGHHGGSAVNPMTKSVEPNGTSIVDVTDPRRPLYL
                     KHLPGGAGVGEAGGAQMVRACNGSELPNANKAKIYLLRATSSSHEVYDVTDPSNPVLV
                     VTIVSNLGDTHKNFWECDTGIAYLVSDGTSLGTSVPSFPAWRAERMTQIFDLSNPAEP
                     KFVRNWGLVGQEPGSTGPVPQDLHGAISIGPKGIRGVTERVYFGHGTGSDGILQIVDR
                     KKLLDPAANGCPPSPNFRTNPTEDDLLCPQLGRMDTSRTMGAHTVFPLLRQPVPELAA
                     QAQNSIRDFVVLVNEAGGGSGASICTGNRQLVYIVDVTTGTSGNESVPTIISNFYVPE
                     NSGDFCNRGGRFGAHASSESFTDLFYGKLIFVSWFNAGVRAVDIREPFSPQEVGFYIP
                     ATTPNTDVRCTNITITPGQPAQEICNFVIQTNNVEVDERGFIYIVDRANTGMHILELT
                     GKAREIMQGQ"
BASE COUNT         6407 a         7715 c         8005 g         6292 t
ORIGIN      
        1 tcttccagcc agcggccctg aaccacggga tccacggtgt tggttgcagc gctgacgata
       61 tcgctgccgc gcaccgcttc ttcggcactc gttaccggca acacctcgat gcccgtgctc
      121 gcgctcatct ctcgggcgaa cttctcacga ttttctttgg tcgggctgta gattttgacc
      181 ttgttgatag gccgaatcgc caggtgcgcc gtgacctgag tttttgcctg ctcaccggaa
      241 cccaacagcc ccatgatttt cgaatcctcg cgggccagat acttgctcgc aacggcgctg
      301 gtcgcagcca cgcgcatcgc ggagatatga tgatcgtcca tgatcgccag caattcgcaa
      361 gtgtccatat cgaacagcat caccagcccg ataaaatcac cgggatagat gcgccgtttg
      421 gcgccatcga tctggacttc tttcgagaag ctggagtcga tgcgcaacgc catggattta
      481 actcccggca ccaggcccat gatgttgtta aaataatact gcccgccctc ttctttccat
      541 ggcgttctca atcgcgcgcg cggcgcattc atcgcttggc ctgcgccaag ttcacggaaa
      601 gcctcctcta tggcatcgat ctcttccgcc atcggcagga gatcttccat ttgttcgttt
      661 ttgagaacca gaaccatgcg ccgggtactc ctcttgttct aagctttctg cctgcgtaca
      721 acagagttag gaactgagtc aaggcgcgcg cagcatgaat tattgcggat ggtttaaccg
      781 cggtcgcgat tcgatcatcg gtctgcggct cggagcgaac gcaacccctg tgaaacgaag
      841 ctgcgactag atggtaaaat cgggagtaaa acggcacagg gaaatagagc gttgccagga
      901 aacagacgag cgagtgatag aatctgcggg cgttaaaaaa gggctgagga ttgcctcagc
      961 ccttggtatg ttgcctaaac aaaatgggat cgctagccct ttttcgcctt gaaccagtcc
     1021 gcgtctattt tgatgaagtg cttccattta tccggcaccg ccgcttcctc gaaaatcgcc
     1081 tccacgggac aaaccggttc gcaagcgccg caatcaatgc actcctcagg atcgatgtaa
     1141 agcttgtttt ccttttcgaa ctcctcggtt tcgtttttag tgggatggat gcagtccacc
     1201 ggacaaacgt cgacacatgc cgtatctttg gtgtcgatac aaggttcagc gatcacatag
     1261 gtcatctatt cttcctctcc tccaatcaga agtgacttta gtctagtagc aaatcgaatt
     1321 atccaagtca attaatcatt tagccaaggc tgccgatccg cttcgtccat aataaaccag
     1381 gcttggacgg tgcgttcagc gccgtcgcaa agcgcatccg agaacgttgc aaaatattga
     1441 acagttaccc ggtcccgatt aggagcatgg cgcggctgca gttgaccgct aagagcgctc
     1501 agtgttagac aagaagatct ttcccaagga gtgtgatcat ggcaaaactt cggcatctgg
     1561 cgatcttgac gcatcaaccg gaaaaactgg cggacttcta taaaaaagtt tttgagctca
     1621 aagaaatgta ccgcaccaaa aacggctcgg tgcatctctc cgacggcgaa gtcaacctgg
     1681 caatcttgaa tgccactgat cctaaagagc cgggtcacgc ggccggcatg tatcattttg
     1741 gttttcatgt cgatgatgcc gaagtcgttg cgcaacggat ccaagagctt catccggaag
     1801 gcgctcccaa agccaggcca aagggaacca gctacgccga gacccgcggt tcggatcccg
     1861 atggcaacct gtttgatatc tccacctggg gctggggtga aaaactgctg cccaaagaaa
     1921 aaggcgggcc gggcgcttga tgctagctta cgttcgatat tcgctgttga acgcttctag
     1981 agcataaggt tcgctctttc atccccgtca cggaacagcc ggcgggcaag caagaagcgg
     2041 catcggtttg ccgctcgggc ctcggagcag catccttaaa aggcgttgac actccggccg
     2101 tgctggaaga cccaagccaa gatggtcatt gagtttgccc gccgcggtgc cggcgcggga
     2161 tatcccaccg cggatctcag aaaaattgcc gcatcaattt tgacggcgct cgagcagagc
     2221 cgggccgaac tcagtctggc cttggtgggt gatcgagaaa tgcgcccgct caacgccaag
     2281 taccgcaaga agaacaagac caccgacgta ctttcgtttc cagccgatcc ggcgatgtgt
     2341 tcggaggcgg gtttgctcgg ggatgtcatc atttccatcg agcaggccaa gcgtcaggcc
     2401 aaggaacgca agacttcctt aaaaatagaa atggccacgt tgttgattca cggcattctt
     2461 catctgttgg gctacgatca cgaacgctcg gagcgacagg caaagatcat ggcgagcctc
     2521 gagcgaaaac tcctcaggca cctttgtgag cgcggcctaa tcaagctata attccaagtc
     2581 gagcaggctg tctaaaagca taggcttaat tgcgctcacc tggaattcgt ttcgtgtttc
     2641 gtgtcgcgct cgttcggaaa tctcgctccc gtttttcacg gacaggatca cgagtattcc
     2701 gcctctgagt ccttttgagc agcctgcaat cagagttttc aacaatctgg acagtgcaga
     2761 atgcagaact acggaatcat tatttagacc gaccaagggc gagggccgat gttagaagaa
     2821 accaaagaac aactggtgca actggaacaa cgactcgata agcttcggag gcgtctttga
     2881 cgttgaaagc aaacagaagc gactcgagga actaactcaa atcacctccc agccggattt
     2941 ctggaacgac gccgacaaag cgcagacgat attcaaagaa cagtcctctc ttaaagctgt
     3001 catagacaat tgggagaagc atcgaacggg cctcgaggag gctcgttttt ttcttgacat
     3061 tgccggcgaa gaagagagcg acgaggcatt gcaggaagcg gcagccaaag tgtcggaggt
     3121 gactcagggg atggccgaga ccgagctggc gcagatccta ggcggcgccg atgatcggcg
     3181 caatgcgatc gtcaccctcc atcccggcgc cggcggcacc gaggcccagg actgggcgga
     3241 aatcttgctg cgcatgtatc tgcgctggtc ggaccgccat ggctaccgca aggaaattct
     3301 cgaataccaa cccggcgaag aagcaggttt aaagagcgtc accttcagcg tcgaaggcga
     3361 ctacgcctac ggctatctga aagccgaagc cggcattcac cggttggtgc gaatctcccc
     3421 ctttgacgcc aattcccggc gtcatacttc gtttgcatcg gtgttcgtct atccggaagt
     3481 cgatgacacc atcaaggtgg aagttgacga agccgatttg cgggtcgata cctatcgctc
     3541 cagcggcgcc ggcgggcagc acgtcaacaa aaccgactcc gccgtgcgcc tcacccatct
     3601 gcctaccggc atcgtggtcg cgtgtcagaa cgaacgctcg cagcacaaga accgggcgat
     3661 ggcgatgaaa attcttcgct cgcgcctgta cgaattggag ctcgagaagc aaaaagaaaa
     3721 aatggatacc ttgcacaaga cgaaaaagga caatgcctgg ggcagccaga tccggtccta
     3781 tgtgctgcat ccttaccgtt tagtcaaaga tcaccgtacc aatatcgaaa tcggcaacgc
     3841 cgacgccgtc ctcgacggcg atatcgattc atttattcaa gcctacctgt tgcacgatag
     3901 tgattcaccc gctgcgtaag atgacttgct cggcgggccg catcacagag acctgcaacg
     3961 ttcaccagtc gaagcgcata taacccaaga gtgttcgtgc acgcttgtct tgcgtcactc
     4021 gcaaatagct gatttcctct tagtgttcca agaaataatc ggtcgaggag gcattatggg
     4081 cggtgaaaac agggaggcgc gcatcggcag tgatgatggc ggcgtccaga atatcgaagc
     4141 agcggttcta gagcgctacg gcgatgcagc cctacaagtg gaagcttgct tgtgcacccc
     4201 cgttagttac gatcgaactc tcctggacat tattcccggc gaaattaatg aaaaagatta
     4261 cggctgcggc gatccatcgc gacacgtccg cgcgggtgag acagtgctcg atttgggctc
     4321 gggcagcggc aaagcctgct atatcatggc tcggatcgtc ggcgccgagg gcaaagtcat
     4381 tggtgtcgat ttcaatccgc caatgttgca gctagcgcgc aagtatcaag aatcgatcgg
     4441 agacaagctc ggttatcata atgtcgagtt tcgccggggc aagattcagg atcttagaac
     4501 gaatctggag ctaatagatc tattcttgca ggaaaacccg gtgcgatcgg tggcggatct
     4561 tgcgcgcttg gaaagctttc agaacacggt tcgcgatgaa cagcctttga ttgccgacga
     4621 gtcgatcgac gtgatcgttt ccaactgcgt cctgaacctg gttcgtcctg aagacaaaaa
     4681 actcttgttc gctgaaatgt accgtgtgct caaacgcggc gggagagttg taatttccga
     4741 catcgttagc gatgagccgg tgccggagca tctctcaaaa gatccggatc tttggagtgc
     4801 ctgcatttcc ggcgcttttc aagaagaaga cttcttgcgc ggcttcgaag aagccaactt
     4861 ttacggtatt cagattgaag aactgagcag cgaaccttat cagaccgtcg aaggcataga
     4921 atttcgcgcg gtcactgtta cggcgtacaa aggaaaggaa ggaccatgtg tcgagcgtaa
     4981 ccaggcagtt gtttaccgcg gcccgtggaa gcaagtcgtc gatgacgacg gccatacgct
     5041 ggagcgcggc gcgcgcatcg cggtttgcga taagacgttc aagctctatt ctcagccacc
     5101 gtatcagggc cagttcattc tgctaccgcc ccggcaagaa gtttcgcttg ataaggccgg
     5161 tctattcgat tgctcgcgcg atcataagcg ccacccacgc gagaccaaag gcgttgggta
     5221 caaagttacc agcgccccca caagcgcctg cacgccgggt tcgacgtgct gtccgtagaa
     5281 gacaggcagg gaaacaagca gaaggaagat aggcaggcaa cgcgcttatg atcttatctc
     5341 cctctcgcgc cacgcagagg accaagtgag gggtgatctt ttatgcaacc cttttcctat
     5401 aagatccatc ctacgaacgg cttgacccgt cgcgccatcg acaccctgca ggtaaacctg
     5461 ggccgttact gcaatctcgc ctgcattcat tgccacgtcg aatcgggtcc cactcgtact
     5521 gagatgatga accgggaaac cgtcgatgcc gtgctgcgat ttctcgccag ctcgaatatt
     5581 tcggatctcg acatcaccgg cggcgcgccg gagctaaacc cgaatttcga ctatcttgtt
     5641 gaatccgcgg tcggtctggg gcgacacgtc atggatcgct gcaacctaac cgtgatcttc
     5701 gagcccggaa aagattatct tcctgagttt ttcgggcgct atggggtgga attggtttgc
     5761 tcgctgccct gttattccga ggaaaatgtc gaccggcaac gcggcaaggg cacgttcgat
     5821 ttaagcatcc gcgctctgca gatcctgaac gagctgggct atgggcggga cgaacattta
     5881 gttctcaatc tggtctataa tccagtggca ccgcacttgc cgccgcccca acagcacctc
     5941 gaacaagatt acaagacaat cttaaagcaa cagttcggca tcgtcttcaa ccagctttac
     6001 tgcctcgcca acatgccgat aacgcgctat gaaactcatc tgaaactgcg cggcgagtac
     6061 aacggctaca tggaactctt ggagagcagt ttcaaccgcg cgaccttgga ccgggtgatg
     6121 tgtagaaatc tgatcagcgt cggctggcaa ggtctcatat acgactgcga tttcaatcaa
     6181 atgctcgagc tgccgatgcg cgacgccgcg ggcaaggcgc tggagatcgc ttcgttgaag
     6241 gtcgagcacc tgctgaaccg cgcgatcacc gtcggtaatc attgttacgc atgcacggcg
     6301 ggttcgggaa gcagttgcgg cggcgcgtta tcctgacttg tcggaagcct tttcgaattc
     6361 gtttcccgat ctcagcgact acaagcgtgc tcgacattga gccgcggcgg agcgctgcag
     6421 aactttgagc tccatcttga gcgatcgaga acaatccgaa agattctgcc tcggtggctg
     6481 ttagcggcgc cgcgccaaag attcgacatg aaaccactca aaatcctcct gataatcctt
     6541 ctgctcattg ccgttggcat agccttagtt tcgtttccgc tccggcattg gtttatgcaa
     6601 ctggaaagtt atgttgaatc cttgggccct gtcggcccga tggtcgtggt gctggcatat
     6661 gttttcttga ccgtgttgtt ggtacccggg agcgccatca ccgtaggggc cgggactctg
     6721 ttcggcttaa aaatcggctt tatcgtggta gtcctcggcg ctaatctagg ggcgctggtt
     6781 gcgttttttt tggcacgaac cctgttacgt caaagagtcg ctcgctgggc tgagagtaat
     6841 ccaagattcc gcttgctgga tcgggccatt ggtaagcaag gattcaaaat ggtcttgctg
     6901 acccggctaa gcccgatctt cccattcgtt ctgctcaact atttcttggg tctgacggcg
     6961 gtccggaccg gcgcttatgt gctcggcaat ttgctcggca tgctgccggc gacttttctc
     7021 tttgtctacc tgggtgcggc agcgcgtgac gcgttagccg gacagctaaa tgtttccgtg
     7081 gggctttatc agcaaatttt gaaagttgtc ggactgctgg caactatcgc ggtcgtgatt
     7141 atgatcactc ggatcgcccg gcaagcgtta cgccaaaccg agcagctcga tgaaggcagt
     7201 acggtcccgg gaaagccgcg cctcgaaccc aattatcggc cggcgacgtt cgataagatg
     7261 atgatcgtcg acgacgcaca cgatcgccgc ctcatccaaa actgccatcc gcctcattgg
     7321 atcaatccgg cgcctgctgg aaagtataat cttgtcgtca ttggcgcggg caccgccgga
     7381 ctcgtcagcg cggccggcgc cgccggattg ggcgccaaag tcgcgctgat cgagcataat
     7441 ctcatgggcg gagattgcct caacatcggc tgcgtgcctt ccaaggcgat gatccgggca
     7501 gcacgcgcgg cccatgatgc gcgcgatgca gcgagattcg gcgtggaaat tgcctgtgag
     7561 ccgcaggttg catttgccgg cgcgatggag cgcatgcgca agcttcgcgc cggcatcagt
     7621 gatcacgatt ccgccgaacg attccgcaag ctcggcgtcg atgtgttcat cggcacggct
     7681 cgtttcaccg gcgcttcatc aatcgaggtg gacggcaaac gtctagagtt taggcgcgca
     7741 gttatcgccg ccggcgcgag ggcggcgaaa ctgccgattc cgggcctgga gaaaactggc
     7801 tacttgacga atgaaacggt ttttacactc accgagctac ccaggcgaat tgcgattatt
     7861 ggcgccgggc ctgtcggttg cgaactggcg caaaccttcg cccgtttcgg cagcaaggtt
     7921 accttgctaa gcgacggcgc ggagatattg cccaaggaag atgccgatgc cgccgcgatt
     7981 gtgcgcaaga aaatggaaca ggatggcgtg cgcatcgtga ccaaggcgaa aattcaccgc
     8041 gccgaaaaaa ataacggcga aaaaatcctc gccgcgaccg ttggggaggc ggctgtcgac
     8101 atcaactgcg atgccgtcct ggttgccgcc ggccgtaccc cgaatctgga aggcctcgac
     8161 cttgaagcag caggcatagg ctatacgtac agaggcgttg aagtcgacga ttggctgcgc
     8221 acgaagaatc cgagagtcta cgctgccggc gatgtctgtt cgcaatttaa gttcacccac
     8281 gcggccgatg ccacggcgcg tctggtgatc gccaacgcgc tcttttttgc gcggcgtaag
     8341 gtttcggctc tgacaatccc atggtgtacc tacaccgatc ccgaaatcgc ccatgtcggt
     8401 tactacgaga aggacgcgcg gcaggcggga tttgccgtgg cgacgctcac ggagtcgatg
     8461 aaagatgtag atcgcgctat tctcgacggc gaggaggagg gatttgcccg cgttcattac
     8521 gatcaaaaga gcggtaagat cctcggcggc gccatcgtag cgcgccatgc cggcgagatg
     8581 atcggcgaat tgaccctcgc tatggtagcg aagcaaaaag ttggggtgtt atcgtcgacg
     8641 atccatccct atccaaccca ggcggaggtg ctgcataaaa tcggcgatgc ctacatgcgg
     8701 gcaagattaa ccccgcgggt aaaaaaaacc ctccagacct ggttgaggtg gcaacgcgtc
     8761 tgaagagaac tcaaagaatt tgcggctcca ggtttgagat atgccagtgt ccgtatcggt
     8821 catcataccc gcgcttaacg aagagaacgc cctccctacg attttagcgg atttaatcgc
     8881 cttgcaaccg cacgaggtga tcgtcgtcga tggtggcagc cgggaccgca cgcgcgagat
     8941 cagcgagcaa cagggtgcca gagtcattac cggcacggcc ggacgcgcgc gccagatgaa
     9001 ccgcggcgct cgtcaagcta ccggggaaat tctgctcttt cttcatgccg acacccgctt
     9061 accgcgcggt gcttttgccg agattgctgc ggctttgaaa aatcccggct gcgtgggcgg
     9121 gcgtttcgat gtcgatttgg acggtaatca ttggctgcta aagataatcg gtgccatgat
     9181 caaccgccgc tcgcgcatta ccaaagtagg caccggcgat caggcgattt tcgttcggcg
     9241 cgaagtgttt gtgcgtatgg gcggtttccc ggatattccg ttgatggagg atattgcctt
     9301 ttgccgcgcg ctaaaacggc tgggcggcat cgcctgcttg cgcagcaaag ttcgtacttc
     9361 cgcgcgccgt tgggaagccg aaggcgtgtg gcgcaccgtg atgaagatgt ggatcctgaa
     9421 gctcctctac tttgccggag tttcgccggt cactttgaag cgtttttatg gcgatgcccg
     9481 ctagcaaggc ggattccgtg atcgtgtccg tggccgtggt cgggaatcca atgaacacgg
     9541 acacgagaac agcaatacga gttgcaagga tgaaagcgga aatctgaaag cggaattcaa
     9601 ttttcagctt tcataattca tctttggaaa ccatatgagt ctttctcggg attctgctaa
     9661 cgctctggtg attttgacca aagcgccaca gccgggcgag agcaaaacca ggctggtccc
     9721 gccgctatct tacgccgaag ccgccgacct tgcgcgggcg ctgctgctcg atcagttgca
     9781 aaacctcgca accttcgatg ccgcgcgact attcatcgcg ttcacacctg aaaacgccgc
     9841 cgtctttttc gaagatttca tcgaccaggg gttcacctgc ttcgcccaac aaggtcaatt
     9901 gttgggccag cgcatgagtc atgccttcca gtatcttttc gctagcggtt ttgaaaacat
     9961 catcctgatt ggcagcgatc ttcctacact ccctgtcgga ttttttcacc agggttacgc
    10021 atcgttggat aaatcagcag acgttgtgct cggtcccagc gccgatggag gctattacct
    10081 ggtcggcctg aaacggatga tcgcggcgat tttcgatgac atcggttgga gcggcgccga
    10141 tgttctctca caaacgattc acaagctcga cgatcttggc gtcaaatacg agcttctctc
    10201 cgaatggtat gatatcgata cagtaaaaga tctggagcgc ttgctgtcac agcgtaggtg
    10261 ccatgaagtt gcgatgaaaa acactttcgc tctattgaac gagcttacgc ggagaggcag
    10321 actgtaatca gaggctgcac tggatgtaca gccctgcttg tggcagtccc accctccgtt
    10381 tccagcttat gattcccggc ggcgcggctc tgtccgtggc ggccgcgagg agatttagaa
    10441 tgcagaacaa aaccgacgcg ctgtgcgtca ataccatccg catgctgtcg gtggacgccg
    10501 tcgaaaaggc gaagtcaggc catccgggcg ctcccatggg gcttgcgccg gtggcttatg
    10561 ttctatggac gcgctttctc aagcacaatc caaaaaatcc gtcgtggccg gatcgtgacc
    10621 gtttcgtctt gtcagcgggg cacggctcca tgctgctcta cagcatgctt catcttaccg
    10681 gttacgatct gccgcttgat caaatcaagc agttccgcca gtggggaagc ctgacgccgg
    10741 gacatcctga acgcgagctg actcccggtg tagaagttac caccgggccg ctcggacaag
    10801 gtttcggcaa cggcgtcggc atggcgatcg ctgaagcgta tctagcggcg cggtataacc
    10861 gtccgggatt cgagatcatc aaccatttta cttatgccat tgttagcgac ggcgacatca
    10921 tggaaggcgt cgccgccgaa gccgccagca tggcaggaca tttgaagctg ggaaaattga
    10981 tttatcttta tgacgacaat cgtatttcgc tggccgcgtc gaccgatctg agcttcagcg
    11041 aagatgtcgg taaaaggttt gatgcttacg gctggcacac gcaatccgtt gccgacggca
    11101 acgatctgaa tgccatcgaa cacgctctcg atcgggcgcg cagcgagacc acccggccgt
    11161 cgctgatatt agtgcgcacg cacatcggct ttggttcgcc gggcaagcaa gattccttcg
    11221 aggcgcatgg ttcgccactg ggcgcagagg aaacaaaact gaccaagcag aacttcggct
    11281 ggccgcctga accttttcat ctgccggaag aggcgctggc gcattttcgt gaatcgattg
    11341 ccaagggtca gaaaattgaa gcgcagtgga acgagcaatg caccgcttac cagagagaat
    11401 ttcccgaact aggcagagaa ctggagcaag cgctgcgcgg cgaactgccg gctgggtggg
    11461 acgcggcgat ccccgatttc cctgctgacg ccaaaggatt ggcgacccgc gccgcttccg
    11521 ggaagattct gaatgccatc gctccgaagc tgcccacttt gatcggcggt tctgccgatt
    11581 taaacccgtc gacttttacc gcgctgaata acctgggcga cttcgaaaat ccccagggcg
    11641 atttctcgaa aagccagggc ctgacgggcg gaggctgcaa ctacgccgga cgcaacctgc
    11701 acttcggcgt gcgcgagcac ggcatgggcg cggccgtcaa cggtatggcg gcgcacggcg
    11761 gcatcattcc gttcggttcg acgttcctga ttttctctga ctatttgcga ccgtcgatgc
    11821 ggcttgccgc tttgatggag ctcggtgcga tctacgtatt cacgcacgac agcatcggcg
    11881 ttggcgagga tggcccgacg catcaaccga ttgagcaact cgccgctttg cgcgcgatac
    11941 cgcggctggt gctgattcgt ccgggcgatg ccaacgaaac cgcggtagct tggagccttg
    12001 ccatcgagac ccgcaaccgg ccggttgcgc tggcactgtc gcgccaaaac gtgccgacgc
    12061 ttgaccgaac gaagtatacc gcagcggccg gcgttcgccg cggcgcctac gtcttggccg
    12121 atccgcccga gggtaaaccg gacctggtgt tgatcggtac cggatcagaa ttgtcgctgg
    12181 tggtagcggc gcgggaaaaa ctgctgcaac agaagatcaa tgcccgggtt gtttcgatgc
    12241 ccagctggga gatcttcgat gaccagccgg aggattatcg tgagttcgtg ctgccgcccg
    12301 atacccgcgc gcgcttggca gtcgaagccg cgctgccttt aggctggcat cggtacgtcg
    12361 gtgaccgggg agacgtactc gccatcgagc gctttggcgc atcggcgccg ggcaatatcg
    12421 tcatggaaaa attcggcttc actgtcgatc atgtcgtcga gcgggctcgc gcgctgttag
    12481 gcaaataacc atgcgcgtgg ccatcggcgc cgatcatgcc ggctttcaat tgaagcaggt
    12541 catcagcgag caccttagat cggcagggca cacgatcatc gatgtcggga cggccagcga
    12601 cgcggcggtc gattatccgg actatgccga agcattggcc aaagtcgttc tcgacggcaa
    12661 agctaagcgt ggcgtgctca tttgcggcag cggtgtcggc gcttcagttg cggccaataa
    12721 aatccccggt gttcgcgcgg gcctgtgtca cgacagctat tcggcgcgcc agggagtgga
    12781 gcacgatgac atcaatgttc tggttctcgg cgctcgcgtg atcggcagcg cgctggcaaa
    12841 ggagctggtg gatcgttttc tagcggcgca attttcccgc gaagaacgcc atctgcgccg
    12901 ggtcggaaaa atcaaagcgt tggagcaacg ctaccaaaaa tcctaaggag agagtcgatg
    12961 aatcctctaa gagagctttt gaatcagggc caatcggtat ggctcgattt catcagccgg
    13021 cagttgatgc gcagtggcga gttgaagcgt cttgtggaag aggacgggct gcgtggtgtc
    13081 accagtaatc cgacaatctt cgagaaagcg attggcggca gcgccgatta cgacgagagt
    13141 ttgagagaaa tgctggcgca ggatcgcaag actcctgtag ggcggttgta cgaacgaatc
    13201 gcgattgaag acatacaggc ggcggccgat gttcttcgtc cggtttacga ccagaccagt
    13261 ggcgccgacg gatacgtcag tctcgaagtt tcgccccacc tagcgcggga tacccaggcg
    13321 accattgccg aggccaagcg gctcaatact tcggtcgatc ggcccaacgt gatgattaaa
    13381 gttccggcga ccgccgaggg aattccggct atcgaagagc tcattgccgg cggcgtcaat
    13441 gtaaatatca cattgatgtt ttcgctggcg cattacgagg cggtggcgca cgcctatatc
    13501 aaagggctcg agcgttgcgc cgatccggcg aaagtggctt cggtggcgtc tttcttcgtc
    13561 agccgggtcg ataccatgat agaccgcgag ctcgagaagc tcggcacacc ggaggcaaag
    13621 tcttgcctcg gtaaaatcgc catcgccaat tccaaagtcg tctaccaacg cttccgtgag
    13681 atttttcacg gcgaaggttt cgtcgcgctg cggcaacgcg gtgcgcgcgt ccagcggccg
    13741 ttgtgggcga gcaccggcac taaaaattcc aactactcgg acgtgcttta tgtcgagaat
    13801 ctaatcggcc ccgagaccgt caacaccttg ccggtagaga cgctgaacgc ctttaaggat
    13861 cacggccggg tgtcggatgc caccgtgcgc caagggctcg atgaagcggc caaggcgctg
    13921 acgcaactca aggaagtggg tatcgatctc gatgcgattg ccgaaaagct ccaggacgat
    13981 ggcgttgacg cgttcgcgac ttcattcgat tcactgatgt ccaccctgga aaaaaagcgc
    14041 agtaccttgg ctggcatcga attgaatcga caggatttgc atcttggcaa actgcaaagc
    14101 cgcgttgatc ggcggctcaa ggcttggcaa gccgaacaat tcggcagcag gttatggaaa
    14161 aaggatccga cgctgtggtc cgccgactcg gctccggagc tgaccgaccg gctgggctgg
    14221 ctcgagatgc cgcagtcgat gcagcagcag gtcgaggcat tgcagagatt cgccgagaaa
    14281 gccaaggctg atggcatgcg tcacgttgtc gttctgggaa tgggcggctc gagcctggcc
    14341 cccgaagtct tccagcaaag cttcggcaat caatcgggtt atccggcctt gatggttctg
    14401 gacagtaccc atccggcggc aatccgatct ctcgaaggaa agatcgatct agcgcgcact
    14461 ctatttttgg tctcgagtaa gtccggcacg acaaccgaaa caaattcttt cttctattat
    14521 ttctgggata agttgaagca gctgaaggcg gaacccggcg atcatttcgt tgcgatcacc
    14581 gatccgggca cggcgctgga aaaattggcc ggcgagcgga aattccgcac ggtttttacc
    14641 gcgcccgaag acatcggcgg ccgctactcg gcgttgacgg tttttgggct cgtgccagcg
    14701 gccctgatcg gcttggacgt cgaggatatt ctaaagcgcg ctcgtcatat gagcgaagcg
    14761 tgcggcgaga gcgtgcctga ggggaagaat ccgggtctgg cgctcggcgc cgcgctggcg
    14821 gagttaactt tggccaaacg cgacaagata acttttctct gctcgccctc tcttagagca
    14881 tttcccgctt ggatcgagca attgattgcc gagagcaccg gcaaggaccg caaaggtatc
    14941 gtgccggttg ccggagagaa actcggcgcc cccgagaagt acggcgccga ccgcgccttc
    15001 gtctacctga gatacgagtc ggatgaaaac catgaactcg atcgccaagt ggcgggactg
    15061 gaagccaacg ggcatccggt gttgcgaatc gagatgcttg aaaagaccga cctgggtcag
    15121 gaatttttcc gctgggagat cgctatcgcc gcagccggag cggcgcttgg gattcatccg
    15181 tttaaccaac ccgacgttca gctggccaag gatcttgcga ccaaagcaat gagagaatct
    15241 cgcgcgggaa atcgaagagg ggccaagctc aaagaagaag tatcctcggc cgatgatgcg
    15301 gcaatgcagc aagcggcttc ttcgtggctg agcaaggcca agccgcgcga ttatgtctcc
    15361 attcaggcct atatcagtcc gacaccggaa cacgatgtcg cgctgcagac tatcagtgaa
    15421 gcgcttcgag atcggctcgg caccgcggtt acggttggtt atggcccgcg tttccttcac
    15481 tccacggggc agctccacaa aggcggtccg aacacagtcc tagtgctaca gatcgtcgat
    15541 cacccgtccg aagatgtgcc ggtgccggaa accgactaca cattcggcgc gctgatcgcg
    15601 gctcaggcac tgggggattt taccgctttg aagcagcggc gccgccgcgc tcttcgcatc
    15661 aacttgggag atgacacgaa agccggattg gagcgtttag ttgtagcgct gcgctaatag
    15721 ccagcgacct atctctgcac gtgttcgtgg tcatgcataa cgtgagcgtg aattcgagag
    15781 attcggatcg aggaacgcca atgagaacgc ggttgcaaga ctttaaggta actgtctgga
    15841 agtcgaaaat catacttcat ctttccgctt ccatgtttag taacttttaa cgggagactc
    15901 acaatggaat taggtatgat cgggctggga cggatgggcg ccaacatgac cgagcggctg
    15961 ttgcgcggcg gccacaagct gattatctac gaccgcagcc ctgaggcgat tcagcgatgc
    16021 gtcgacaaag gcgcagtcgg gacgcattcg ctggccgact ttgtcaagca actcagtttg
    16081 ccgcgagtca tctggctgat ggtgccatcc ggcgatccag tggatctcac tatcgagcag
    16141 ctgattccta gtctctcgaa ggcggacatc atcatcgatg gcggcaactc acattataag
    16201 gattccatcc ggcgcgcgga aaaactcaaa caacttggca ttcacttcgt cgatgccggc
    16261 accagcggcg gcatctgggg tttgcaagaa ggttattgcc tgatgatcgg cggcgaaaag
    16321 gcgattgtcg accgcgtcga tccgattttc aaaacactcg cgccggagaa tggtttttgt
    16381 cacgtcggac cgagcggcgc cggacatttc gtcaagatgg ttcacaacgg catcgagtac
    16441 ggcatgctgc aagcgtatgg cgaaggcttt gaaatgctga aagcctccca gttcgacctc
    16501 gatctggggc aaatttcccg cctatggaat caaggcagtg tcgtgcgttc gtggttattg
    16561 gagctggcag aaaatgcatt tcagaaagat ccgcagctct catccatcaa aggctacgtc
    16621 gaagattccg gcgagggtcg ctgggcagtg ctcgaagcgg tggagcgaga gattccggct
    16681 tcaattctga cgctctctct ctttgcccgg tacacctcgc gccaggagga ttctttttcc
    16741 gctaaggtga tcgccgctct acgaaacgag ttcggcggcc atgccgtaaa aaaggagtaa
    16801 ctttcatggc aagcgacccg caggactatg ttccgagcgg tttcagtcag gcgaaaaaac
    16861 cccattcgtg cgtgctggtt attttcggtg cttccggcga tttgaccaag cggaagctga
    16921 tacctgcctt gtacaatctc gcgttggaaa agcgcctgcc ggagcgcttc tcggtggtcg
    16981 gtttcgcccg cagcaacatg agcgacggcg agttccgcaa caaaatgcgc gaagcggtga
    17041 gcgagttttc gcgcaccggg ctgaaggatg aacaggtgtg gcaacagttt gcctcaacgc
    17101 tctattacgt tcgcggtgat tataacgagt ccggcgcata ccagcgtctc aagagcatga
    17161 ttgacggctt tgatcgcggc agccgtgtcc tgccggtgcg ggttttctat ttagccatgc
    17221 cgcctgattt gtacgctccg gtaatcgagc gcatggccgc ggttggatta gctccgaagg
    17281 aaagcgaggg taccgcgcgg actcgggtgg tgatcgaaaa gccgttcggc agcgatctcg
    17341 aatcggctcg ggaactcaac cggcgcgtgc atgaggcgct ggatgaaaag caggtctatc
    17401 gaatcgatca ttatctcggc aaagaaaccg tgcagaacat tatggtgctt cgcttcgcca
    17461 atgccgtgtt cgagccgatc tggaatcggc gctacgtcga tcatgtgcaa attaccgccg
    17521 ccgaaaccgt cggcgtggaa acccgcggag ggtactacga caaagccggc gttgcccgcg
    17581 acatgtttca aaatcatctc ttgcagcttg cttgtcttac cgcgatggag ccgccgacag
    17641 gcttttccgc cgatgcggta cgagatgaaa agagcaagct gctgcgtgcc gtgcgcccga
    17701 taaccgacga tcaagtggcg ctccacgcgg tgcgggggca atacggtacg ggccgaatca
    17761 atggtaaacc cgtgccgggt tatcgcgaag aatccgacgt tgccagagat tcgtcgatgc
    17821 ctacttatgc ggccatgaag ctcttgatcg acaactggcg ctgggaagga gtaccttttt
    17881 acttgagatc aggtaaacgg ctgttcaggc gcgtgaccga aattgccatc caatttaagc
    17941 ggcctcccct attgctgttc aaatcctgcg ctgttgagga tgtggctccc aatgtattgg
    18001 tgatccggat tcagcccgac gaaggcgtgg cgcttacgtt cgaagtcaaa ccgccaggac
    18061 cggacatctg tgtgagtccg ctcaaactcg atttcaagta cgaagaggca ttcggcaaca
    18121 cttcgccaga ggcatacgag accttgctcg aggattgtat cgaaggcgac tccaccctct
    18181 atacgcggca cgactgggtg gagctggcgt ggttgcttat ggatcccgtg ataaaaacct
    18241 gggaaagaat ccagccgaaa gatttcccta attacgaggc tggtggctgg ggaccgaagg
    18301 acgccgatga attcatgcag cgcgacgggc ggcgctggcg gcggccgtta aataatacca
    18361 atcagcctgg ctaagttctg aaagcacacg atgatttggt ctcgaaccca tcactccacc
    18421 acgccagtac tccagccccg atgaggcgcg agctcccctt cgacaatgct cagggcaatc
    18481 ggcatatgag tccttttcag cagcctgata gagaattgat tttagccggc gacattggtg
    18541 gcaccaaaac taaccttgcg ctcttctcct atcagaacaa caatcttaaa gccgaatttc
    18601 aaaaaacgta ttcgagccag gagtattccg gcctggtgcc gatcctggaa gagtttttcg
    18661 ccaccggcaa acatccgatc agccgagcgt cgttcggcat tgccggtcca ctggtcgatg
    18721 gcaaagtgaa tccgcctaat ctggactggg tcgtcgatcc cgtatcggtt gcgaaaaccc
    18781 tggagttgcc ttcgatcgct ctgcttaacg atttggaagc cgccgcttac ggcattttta
    18841 cattgacacc ggacgagttt ctcgtgctca atgaagggat cgtcaggcgg ccgggtaata
    18901 aggttctcat tgccgccggt accggattgg gagaaaccat tcttttcgat gacggcaagg
    18961 attattatcc gttagcttca gagggcgggc atgccgactt cgcagcgaga aatgaactgg
    19021 agattgaatt gttacgctac ctgatcggtc gattcggccg cgtaagttac gaacgggttg
    19081 tctcgggtcc tggcctggca aacatctacg attttttaaa agagaaggtc ggcttgtcgg
    19141 tgccgggccc gttcgcggac aagatcgccg ctgcggagga tcgcagtcct ctcatatcac
    19201 aagccgctct ggcaggagcg ccggagatcg ctgtcaaggc gttggatatg ttcgtatccg
    19261 tctatggcgc tgaggccggc aatctcgcgc tcaagggcaa agccattggc ggcgtttacg
    19321 ttggcggcgg cattgccccc aagatgctgg acaagctgaa ggacggaacg tttatgcaag
    19381 ccttgaacga caagggtcgt taccgggagc tggtttcgtc gatccccgtt tatgtcgttc
    19441 tcaacgaaaa agccgcgctg cgcggcgccg cttaccatgc cactctgggc cgcagtggct
    19501 gaggcttcgc atggctgagc gggatatcgt tctctgtcgg gatcttgttg aactaagtca
    19561 taagagcgcc gaacagttta gccagcttgc ccgtgaatcg atagaagcct cgggacgctt
    19621 gaccgtcgcg ttttccggcg gctcgacccc taagcatctt tactcactcc tcgcctcacc
    19681 ggcctataaa aaccgaatcc agtggaacaa tgttgagcta ttctggggcg atgaacgttg
    19741 cgttccgccg gatcacacgg agagcaattt tcgcatggcg caagaagcgc tcttgtcgaa
    19801 tatcgaaatt ccagcggaga atattcatcg gatgcgcgga gagagacaac cgcaagcggc
    19861 ggcagccgag tatgagaaag aactacagaa attctttggc ttggactccg gggggctgcc
    19921 gcgcttcgac ctgatcctcc tcggcatcgg cgaggacggc catacggcat ctctttttcc
    19981 cggtagcgac gcgctggccc cctccttcat cctcccgcgt ttgacggggg aggaaagagg
    20041 agggggagtt gcccatgatc gcccgtcgca gagtcaccgc ggtcacagtg aaactgaaac
    20101 cgtgcatctg gttgttgctc ccttcgtcga aaaactaaaa tccttccgac tgactctgac
    20161 cttgccggtt ttgaacaacg ccgctgacgt ttggtttctt gtagcaggtt ccagcaaagc
    20221 cggcgctgta aagcaatcct tcagcagttc ttccgatctt cccgccgcaa aggttcagcc
    20281 ggtgaatgga aaacttacct ggttcatcac gcgagatgcg gcaccgggaa ttccagcagc
    20341 tggtaaatag cacatcaaga tcgcttctaa atcacactga cgaggatcgc tcctaacctt
    20401 aataagattt cggtggcatt gcagccgaac gcatgttatc ggtcgggcgt agtgtgagtt
    20461 gagacaaacg cgacaactca cgcgggggga aatcgtatga gtggaaagct gctggttgtc
    20521 ctgacattta tcctttgcgc cgtaagtcgg ccgcaggcgc agacactcga tgaagtatac
    20581 aaaagggctt tgacagaagg cggcacgctc aacttctatg cgacgttggc gcaggttaac
    20641 gccgctagca ttcttcccgg attcgagaaa cgttttccag gtatcaaggt caatcacgtt
    20701 gatgcaacat ccgatcagct cgcctcccgg gtcattgccg aatcccgcgg cggcaaggtt
    20761 gttgccgatg tttttcagac acttctggac ggcatgattc gcgtccaaag ccagggcctc
    20821 ttccttgaaa aattgtatcc tgaggccgct gaatatccag tagagctcaa gggaacaacc
    20881 tgggtggcca ccgatctgat ttacatagtt gcggcgtgga acaccacgaa gatcaagaag
    20941 caggacgagc cgaaacagtt cgatgatttt gccgatccac gctggaagaa tcagctcatc
    21001 gccgagcctc gagatttcca gttattgttg ggtctggcga agcacaaata caaaagcgac
    21061 gagaaggcgg ttgccttact caagcgaatt gccgataaca acgtccagtt ccacaaaggc
    21121 cattcgcaac tggccgagtt gctcgtcgcg ggacaagccg cggcctgcgt tacctgttac
    21181 tcgcatcact acccgagtcg aaaacgaaag ggcgccccgg tggattactt gttgaccgaa
    21241 ggaaccggcg acattgacgc caccgcgatc atgaagaatg cgccgcatcc aaatacagct
    21301 ttgctgtttg cgcgttggac cgcgagcgca gacggacaaa aaatctatgc gcagggtggc
    21361 cgccttccgg ctcatcccaa cgtcgaagcg gtagaaaaaa cccgccccac gaaggtctac
    21421 ccgttaggcg agcaagatgt caaagactat cccaagtacg aaaagatgtg gaaggagatc
    21481 ttcgggctgc gataacttga atgatggcac ccaattcaat tcggcgctac attccatcca
    21541 tgcctgggaa atgctcacag tgcggctcga ggacttattt ggctcgaaca atgatccaca
    21601 atgattcaat ggagatccaa aatatcccgt gcacggtttg ccatgaatgt gactatgagc
    21661 agatcggcaa gcaagttcag aagaagatcg ataagcttct cgaacgcgcc gccagaggga
    21721 aactcaagtc ccgcttggtc gtgatgtagg gggagtcgta gattatttcg gacacttcat
    21781 ttcggcaaag ttatcgccaa aatctttaca gatcgctgca agtgtctcct tgtcttcctt
    21841 tatcagcatc tcagccgcat tgaacaacgc ctcttgtgct tcagccgcgc ctaatccatg
    21901 aggcgccagc agggtgtagc gggtcggaat gttatttacg atcgcgctaa ttgtccctgc
    21961 attcggcaag tgtgttagat gctgcagtct ttgaaacgtg atcagccaag cgtgacactc
    22021 aacgtctttt agttttggat ctttctttaa ttcaggatca tccgcttcag cgaagagatg
    22081 gaatggctcg tatacatcag gcgccctgtt tccgtccgag ctgattccca tctcctccaa
    22141 tagtctatcc cttcgtaacg cgagcagcac gctacttgcc tccaaagcat tggtgtcgat
    22201 cacgaagtcg gtgatcgctt ttcgcgtatc ggggtcttca cgggataaat tccatccttg
    22261 agtgtacgca tcgactatca ggaccagaca tttcctgggg cgctcgcttt caggtcctag
    22321 atacagatct cgggcgactt tgcgcaggct gttgatgcct aagttatccg aggggccgcc
    22381 atcaaataga tggacgtatt gctttggttg cgtatagtca cggagcgtga cgttgtgaaa
    22441 ggctcccgga aaagcgccgg aggccatcac agctctcgaa accgcatact tatcgagccc
    22501 ggatccaatt ttgttgaaag cctcgtccgt gaaaagatac gacgctccgt tgtcggttaa
    22561 tgacgtggcg ttgataagga ttttcggggt ggttctgggc atctgcccga atgttttgtc
    22621 ttcaaataga ttgtggtcga aaacgtcggc catgatgtcg ctccgatcaa acccggtaaa
    22681 ccagtaacgc cctatgttcc acggtagaaa ccatcgccaa aaccactcaa gctcgaaatt
    22741 tttcaccaat cgttcgcgaa cttgttgcgc attccaatga tgaatgttgt cgtcgctgaa
    22801 gaggccgtaa taagcagccg tcagggaact gccggagacg gacgaaagag cggtcgcttg
    22861 ctgcagtatt cctaagctct cgagttggag tagtgcagca gcggaaaaat tcgccgcgcg
    22921 acttccgccg ccggacatcc caattcctat aaaggtgcca ttcttcaaat ctgacgacga
    22981 gattttggtc gtggctctcg tcggagcggg cgaggttagc acggggcctt cgttggcgtg
    23041 agttccatat tggataatgg cgcacccgga aacaaatgct agatggaaag ccgccaggat
    23101 tattatcagt cccgtatata tcacttgggc cataaccgat ctccgttcgg aatgagttat
    23161 tcgaagttcg agtgctatct tacagacgcc gggctatagt gacattcagg caaacccttg
    23221 gggttattgg aggtagtgag cgctaggttt tgagttcgta cacgacttgt ccgtggcagc
    23281 gtgagtcgtt cagggatgtc atatagagca gatagcaggc gacggtcaag aaaaaaatcc
    23341 ctagcggcag atgcgccagg agaccgcgag agtctcacac tcatattcta tgcccttttt
    23401 cgattattct cccgcaagcg tatgagatta ctgttggcac gaagttgtcg ggttgctatc
    23461 ggaaaaagaa atgcattggc ctgttttcgg ttggtaaatc gaaaatccgt agttgttgga
    23521 agtattaagg agccgcagcc gttctacaaa gccgtcataa ttagcgaacg gcaaggctct
    23581 catgccgttg ttgggatctt ctcctgatat gtaaggtgag ccgaagtcat cataatgact
    23641 gagcacgatg ttttcaggcc ttaacagccg gagaagagtg tccggatagt catctacttt
    23701 gtcccatccc ggaacgcata agatggcaat attgactcga tgctcattca gaagactttg
    23761 atacttgcgc agcgcttcag cgcctgcggc gctattagca gcaccgttga tgaaaatacg
    23821 ccaaagcacg ctctcctgat catctaagaa gtcgaccagg aaaatcaagg tttgtccttc
    23881 caaatattgg ccaacccggg aagggatcga tgtcatgggg ctaactacat cacccgccat
    23941 gaatgtatgg ccaaagagat gcggcgcatg gtcgctagct aaagcggtca ctctaattct
    24001 gccttttatg atcgatggct tctgttcggc gatgacgaaa tcaagtgccg gtggtttaaa
    24061 agccaatagg atgtttcgtg cagtgagact gccgatatag gtgacacttc tcccggagtt
    24121 ccggatgatg tagtagggaa cgtccagcag atgatcgtag tgcccatggc tcaagagaac
    24181 gaatctggta tttccgggta gctccggaag caccgctctt attcgctccg ggtcataggt
    24241 aaagttactt gtaagatacg gcatcatcag agaagcgctc acacgcgcga acgaaggacg
    24301 cgtgaaaaaa gggtccacaa ccacgacatc atcgccgctg gagagaatcc aggaagatgt
    24361 gccgagccac tgaatatggc ggctcgttgg cgccggtgac tccgcggcca aagcgggcga
    24421 agtgacaggc gccaggacgg cggcgccagc gctcaagaca atcgcgcccg tcagcagcga
    24481 aagcagcagg cgtttcaatt tatttgattg cccatgccgc ggttttgaca atattaaagt
    24541 cagaaacgta agctcggtaa ccatgatgca gtgttcgaag tctcgagctg tttactccga
    24601 atgccggcca ataaacccga gcacggtgcg attgaagaaa tcggggcgct cccagtagat
    24661 cgaatggccg cattccgccg cgatcacgac ctcgttgtcc ggaacgtgcc gcgctatcat
    24721 ccgggtgatt gcaggcggcg tgaacatgtc ggctgcgccg gtcatcagca aggtcggcac
    24781 tttcaacgtc tgcaacttct ccggcgtgac gacgttgcag agcttttggc gcgcgcctct
    24841 gccgagctcg gacttatgat tgagctcgat ccacttggta acgcccccag gatttgccgc
    24901 ccgataggac ggcccgagtt cgcgaaacca gcgcggcagc tgatcccact ccggcagctt
    24961 gatcctggcc gcggcttcgg cgatgtaacc atcggcggcg gccaggttgt tgctcgatat
    25021 cgttaagctt agcagccgtt ccggatgaga caaggcataa tctgcggcga cactgccacc
    25081 agcagccgag ctcacggcat gaaacttttt caggccgagg tactcgataa agttatccag
    25141 gtcttccgat ccgcttcctg gattgtcgtc cggcgccgga tcggaattat gatagttgcg
    25201 acgcgagtaa gcgatgacgc ggtagccggc accgctgaac accggctgct gatacaacca
    25261 gatcagcgcg ctccccgatg ctggatgcag aaacacgacc ggctctccgg cgccgcccgt
    25321 atcccaatag ccaagatgcg tgccgggaag ctctgccatc ccgtctttcg ccggagcctg
    25381 ttccggtatc ggcactggaa gcaacgggtc gtccaactca gtggcgggaa tggtctcgtt
    25441 catcatgttc gttgtaccta ttctataaca ttggctggaa ttcgcgggtg aaatcttaat
    25501 tttcagcgga ttcccgtcgt ttgatactca tggtaactac ctgactgatc catcggcaac
    25561 ccatcggtca tgagtggtaa taaaaatata aaaaataaag cttgagctaa atgaatgcgg
    25621 gcggtaagct tgcccgccgt atctactgaa ctgcggtcta cccgcaaagg agagtgttat
    25681 gaaccctatg ttcattgtcg ccattcctat agtcttgata gtcattggca tggtgctcaa
    25741 tagcaccggg ctttcattcg atcagccgcc gagctcatcg gagcaagatc ctgcaaagaa
    25801 aagcgccgcc gacgcagatg cttaccgccg attcttcgat gtacaacgaa agcaagctgt
    25861 caaaaggcag gcgcgagtcg gccagtacgc ctggctgcta atggccgcca tcatcggatc
    25921 gttcatctgg ctgtacatgt ataccgtcag taaaaccagc ttggccacac agatcggcgc
    25981 ggttcagacc ctggccactg aagatggcaa agaggcggtt ctttcaatca ctttgaagga
    26041 cgggagcaat gtcaaatacc tgatcaagcc ggccacagcc gaaatcatag aagccagcaa
    26101 gaaagaggga aactccaaag agaaaatatc atcctacgag atgtcgcaat taggcaccgc
    26161 tttcaacgtc ggcgaaagtc ccttatcgcc gggtatcgcg atcaagcttt ccaaatgatg
    26221 agaggcagcg tcgactgaac ttattcagac aagcgttatc cagtgaagtg cttcgtcgct
    26281 gatcgaaggt cgggctcatc acaatcgatt ccttcaactc gacaggagcc cgcggctact
    26341 ttgatgactt gcgagagagg gaagcgccat caatgtagcc gctctcccga tcctggttat
    26401 tgcccttgca taatctctcg agccttgcct gtaagctcca ggatatgcat cccggtgttg
    26461 gcccggtcca cgatgtagat gaacccccgc tcgtcgacct caacgttgtt ggtctgaatg
    26521 acaaaattgc agatctcctg agcgggctgc cccggtgtga tggtgatatt cgtgcagcgc
    26581 acgtcggtgt tgggcgtggt cgcgggtata taaaaaccta cctcctgagg gctaaacggc
    26641 tcccggatgt ccaccgccct tacgcccgcg ttgaaccatg acacgaaaat cagctttcca
    26701 tagaacaggt ccgtgaacga ctcgctgctg gcgtgagcgc caaaacgacc gcctcgattg
    26761 cagaagtccc cgctattctc gggaacatag aagtttgaga tgatcgtcgg cacggactcg
    26821 ttgccggagg ttccggtcgt aacgtcgacg atatagacga gctgccggtt gcccgtgcaa
    26881 atgctggcgc cgctaccgcc tccggcttca ttgacgagaa cgacaaagtc acgaatcgaa
    26941 ttctgtgctt gagcggctag ctctggtact ggttgccgga gcaaggggaa aacggtgtgt
    27001 gcgcccatgg tccgcgaggt gtccatgcgg ccgagctgcg gacagagaag atcgtcctcg
    27061 gttgggttgg tccggaaatt aggcgaaggc ggacatccgt tagcagcagg gtcaagcaac
    27121 ttcttccggt cgacaatctg gagaatgccg tctgagccgg tcccatgacc gaagtagacg
    27181 cgttcggtca ccccccttat gcctttgggt ccgatagaga tcgcgccgtg aagatcctgc
    27241 ggcaccggtc ccgtagatcc gggctcctgc ccgaccaaac cccaattgcg gacaaatttc
    27301 ggctccgccg gattgcttag gtcgaaaatc tgggtcattc gttccgcccg ccaggccgga
    27361 aacgatggca cggatgtgcc taagctggtg ccgtcagaca cgaggtaagc gattccggta
    27421 tcgcactccc aaaaattctt gtgcgtgtcc ccaagattgg aaacgatcgt aaccacgaga
    27481 accggattgc tcggatcggt gacgtcatag acctcgtgcg agctggaggt cgcgcgcagc
    27541 aggtagatct tagctttgtt tgcattgggc agctcgctgc cgttacaggc gcggaccatt
    27601 tgcgcgccgc cagcctcgcc gactcccgcg ccaccaggca ggtgtttgag atagagcggc
    27661 cggcgcggat cggtgacatc gacgatcgag gtgccgttcg gctccacgct tttggtcatg
    27721 ggattgacgg cactcccgcc atgatgcccg atataggcga tccagcgatt gccctgtttg
    27781 tgaatgattg gctggtatgc ggagcgcgcc tgcaggttgt tgaagccaac aagccgcatg
    27841 ttttttgcct ccggcgtatc gccgattgcg agcgctaagc tgatgttcaa ggcgagcaag
    27901 gcaatgacag taaatggaag aaataaaaca acaaaactta gtagtcgctt catgaaagcc
    27961 tccgttcttt caccgcgcgt tattgttgtt tacctcatca tccctgtgcc ggttgctttt
    28021 aaacctcctt tctctttacc cctggtgttc tttaaccatc cccttctgtt tatccttttg
    28081 cttttgtctg agctcttttt ttcgcgccgc ttcactccgc tctctctccc cctctctccc
    28141 cctctccgaa tgatcccgtc ccggcgcggg atatagcacc agcgactttc agatgtcaat
    28201 caactttttt tctacgacca agaacgtctg ctggagatcg gaaagatttc cgttacttcg
    28261 ttggtctcaa tcgttttgac atcgatggcg cgctatataa agtcatcgcg tatcgcccga
    28321 ttatcattcc ttcaactctc ggaggaagct atggaaaatt acaactacga ttcattcgga
    28381 cagtgtgaag acggtgcgcc ggatttttgc tcgttgcac
//
LOCUS       DASUVS010000125         2876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_264705, whole genome shotgun sequence.
ACCESSION   DASUVS010000125 DASUVS010000000
VERSION     DASUVS010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264705"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VF182_07120"
     CDS             <1..481
                     /locus_tag="VF182_07120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6436884.1"
                     /translation="EHNSAYYVDGYMGAALWLPPDVHPDENALVELLKRSIPEENQAE
                     IFAFMEEMDSSHPSEPHWYLPMIGVDPAQQGNGYGSALLKHSLGRCDAEGKLAYLEAS
                     SPKSVPLYRRHGFDLVGRIQVGSSPPLFPMLREPRRQVMARHQDYPSPTPIETEQLY"
     gene            478..699
                     /locus_tag="VF182_07125"
     CDS             478..699
                     /locus_tag="VF182_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436885.1"
                     /translation="MKGAFMEKLGIVVRDDAYDKILTPLTFAYVQAKQGVKVDMLFVL
                     WAVGALTEDGARSLTVDGMPPNRNGCDNA"
     gene            1258..1743
                     /locus_tag="VF182_07130"
     CDS             1258..1743
                     /locus_tag="VF182_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6436886.1"
                     /translation="MLRYNPDELATIPAECAASFSGLGNPHRIGPIEPGEAILDIGCG
                     AGMDLLLAARRTGASGRAIGIDLTPAMIEHAKCAALKTRLWQNVTIRRGAAEELSVQS
                     GTIDVVISNGMMNLSSDKRRLFSEIYRVLRPGGRLYLADVVVQRELSLSARSDVDLWA
                     G"
     gene            complement(2096..>2876)
                     /locus_tag="VF182_07135"
     CDS             complement(2096..>2876)
                     /locus_tag="VF182_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="catalase-related domain-containing protein"
                     /protein_id="HEX6436887.1"
                     /translation="EEEGAKLRVRSETFADHYSQARQFYISQTDTEQNHIANALIFEL
                     SKVERVAIRSRMVSHLLNIHSDLAKQVADGLGLKELPKAADAARPTREDLKKSPALSI
                     LLNGPKSFTGRKVGALVTEGVDIKILESLKSALKSEGALLEIVAPKVGGVKASDGSWI
                     EAAQKIDGGPSVLYDAVALLPSEDGAKMLGKEPAARDFIADAFAHMKFIGYTDGAISL
                     MMHAGVPETRDKGFIAIKGQDDCSSFVAACRQLRFWDRAKI"
BASE COUNT          637 a          784 c          808 g          647 t
ORIGIN      
        1 tgaacacaat agcgcctatt atgttgacgg ctacatgggt gccgcgcttt ggcttccccc
       61 agacgtccat cctgacgaaa acgcgctggt ggaattgctt aaacgctcga ttccggagga
      121 gaaccaagcg gagatctttg ccttcatgga ggagatggat agctcccatc cgtccgagcc
      181 tcactggtat ttaccaatga ttggagtgga ccccgcccaa caggggaatg gctacggctc
      241 ggcattgctg aaacattcgc tcggaaggtg cgatgccgaa ggcaaactcg cctacctcga
      301 agcgtcgagc ccaaaaagtg tcccgctgta tcggcgacac ggctttgacc tggtcggcag
      361 gattcaggtt gggtcgtcac cacccctgtt tccgatgctg cgcgagccgc gacgacaagt
      421 gatggcgaga caccaggatt atccgtcgcc cactcccatc gaaacggagc agttatattg
      481 aaaggagcgt ttatggaaaa gctgggtatc gtagttcgtg atgacgcgta cgacaagatc
      541 ctcacgccgc ttaccttcgc ctacgtacag gccaaacagg gcgttaaagt ggatatgttg
      601 ttcgtgctct gggctgttgg cgcactcaca gaagacgggg cacgatcgct tacagtggac
      661 ggcatgcctc cgaatcggaa tggctgcgac aacgcatgag cgctgatggt gagccgacgg
      721 agatgtatga ctatctgaag ctgctcgcag ccactggtaa cgtaaagctt tacggctgcg
      781 agctagctgc ttccaccttt ggcgtgaagt catcggacct tatgccggag gcagatggaa
      841 tcgtcgaccc cggctgttct tgaatgaaaa gtcgctcaaa gcagaccatt gtcagtactt
      901 gattcattta gtgaaagatc tctcacggag tttaccctga atattcggaa ggtttgagat
      961 gacagttgtc gtgtttaggc cattccgagc agagatgcga gaaatctttc cccaggtacg
     1021 atctaaggaa cctcaaagga accaccgctc agtctcagta tcggcgcgcg gaatccctta
     1081 ccaatcgact gcaccgggct gaaacttcga cgtaacgaga aaacgatggc agctagttgt
     1141 ccgagcagct ttgatatagc acgtttacgc aaacaggtaa gccaaaccta tgatcggctg
     1201 gcacgagagc ccgacggtaa ttttcatttc catcgaggac cggagtacgc atctcgcatg
     1261 cttcgataca acccggatga attggcgacg attccagcgg aatgtgccgc atctttttcc
     1321 gggctcggca atccgcatcg tatcggtccc atcgaaccag gtgaagcaat tctcgatatc
     1381 ggctgcggcg ccggaatgga tttacttctg gccgcgcggc gaaccggagc ttcgggccgg
     1441 gccatcggaa tcgatctgac gccggcaatg atcgagcatg cgaagtgcgc cgcgctcaag
     1501 acccggttgt ggcagaacgt tacgatacgg cgcggcgcgg ccgaggaatt gtcggtgcaa
     1561 agcgggacga tcgatgttgt gatttccaac gggatgatga atttgtcctc tgacaaacgc
     1621 cggctgttca gtgagattta ccgggtgctg cgcccgggcg gccgcctcta tcttgccgac
     1681 gttgtcgtgc agcgcgaact ctcactcagc gcgcgcagcg atgtcgatct ctgggccggt
     1741 tgaatcggcg gcgctctgcc ggaggcagag ctctatcagt tgaccgcccg gatcggtttt
     1801 gttgacgggg aaatcgtcga acgtttcgat tgttatgaag gaacgtcggt caaagccaaa
     1861 ttatcgaaag atctttacgc gcaggggata aactttttgg cgcgcaagcc tgtcagctat
     1921 tgacaatccg cagtacgccc gccggccagc cttagacgcg attaccagct ctgttaattt
     1981 caactctaac gacgtactcc cgttatcgtt gcgagaaggg agtacagagc tgtggcttag
     2041 agccaacgat cagcctgata ttaacatcca aagatcaccc acggttgagg gatgactaga
     2101 ttttcgcgcg gtcccaaaag cgcagctgcc ggcaggccgc aacaaaggat gaacaatcat
     2161 cctgtccttt gatggcgata aatcccttgt cgcgggtttc gggcactccc gcgtgcatca
     2221 ttagactgat ggcaccatcc gtgtagccga taaacttcat gtgcgcgaaa gcatcggcga
     2281 tgaagtcgcg tgccgccggt tctttcccaa gcatttttgc cccatcttcc gacggcaaca
     2341 gcgcgacggc gtcgtaaagc accgatggcc cgccgtcaat cttctgcgcc gcttcgatcc
     2401 aagaaccgtc gctggctttg acgccgccca ccttcggcgc gacaatttca agcagcgcgc
     2461 cttcgctctt cagagcggat ttgagcgatt ccaagatctt gatgtctacg ccttcagtga
     2521 cgagggcgcc taccttacgt cccgtaaaac tcttggggcc gttgagcagg atgctcaagg
     2581 ccggcgactt ctttaaatct tcgcgcgtcg gcctggcggc gtctgctgcc ttaggcagct
     2641 ctttgagacc caaaccatcg gccacctgtt ttgccagatc cgagtgaata ttcaggagat
     2701 gcgagaccat gcgactgcga atggccactc gttcgacctt gctgagctcg aagatgagag
     2761 cgttggcgat atggttctgt tcggtgtcgg tctgactgat ataaaactgt cgagcttggc
     2821 tgtagtggtc ggcgaaagtt tccgaacgaa cgcgcagttt ggcaccctct tcctct
//
LOCUS       DASUVS010000126         4613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_701764, whole genome shotgun sequence.
ACCESSION   DASUVS010000126 DASUVS010000000
VERSION     DASUVS010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701764"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VF182_07140"
     CDS             <1..232
                     /locus_tag="VF182_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436888.1"
                     /translation="ISIIERAPHLNTAKLFIEYVLSEEGQRLLAKRGREPARPGIRPE
                     GYPTTLKVVPSRVELAEKLADYTRRYESLFVK"
     gene            complement(371..829)
                     /locus_tag="VF182_07145"
     CDS             complement(371..829)
                     /locus_tag="VF182_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6436889.1"
                     /translation="MLPQNTIDGITLHIRHTMLPVSNMDRTVDFYSRLLGMDIQRIRD
                     VPDRNERVRYLGYGSEDEGPSLELIESVESRGKAPLAQWTGHIALYVSDCYKLCEKLK
                     AEGVEFVSGPGPNRPGGNDIYAFIKDPDGYVVELTERHAKTGPVVGRASE"
     gene            complement(882..1712)
                     /locus_tag="VF182_07150"
     CDS             complement(882..1712)
                     /locus_tag="VF182_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX6436890.1"
                     /translation="MYQPKSVAEASEMLGEFGEKGRLYAGGTELLLAMKHDLLRYEHL
                     VDVKTIPGLDKIESKNGALFIGGTATHRAIERSPLVQQHLPVLAQMEKNVANVRVRAS
                     GTLGGNLCFAEPHSDPATLLLALGAKAHLQAKSKRRTLTIDKLITGSYETSLASDELL
                     EAIEIPIPTTSQRAIYLKYQLKERPTLGLALVLDVDGENIKQASAVVGSVSAVPTQSE
                     KTNRLLEGSRAQVEAQLADAADALAQAADPVDDLEGGADYKRHLIGVFLKRAFVKALS
                     "
     gene            complement(1805..4084)
                     /locus_tag="VF182_07155"
     CDS             complement(1805..4084)
                     /locus_tag="VF182_07155"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6436891.1"
                     /translation="MSAEKKTDFHVVNHSLPRRDGRVKVTGKAQYVADLKLIGMAYAK
                     VLRSPYAHAKIISIDTSRARAHPGVFCVVTGNDLEGLNPYYGHAVKDHPLLAIDKVRY
                     AGEPVAAVVAVDERTAFEALELIDVKYQELPAVFTPQEALAKDAPLLHERQFEAGALR
                     GFEGEVTAGKGSNICQKHEVRWGNVEEAFKNAAAVVEAEYYFPMTYAYAMEAYVAVAD
                     VTDQGVNIYSSAQHPFMVRHDLKTVFNLPVSKVRLIVPFVGGGYGSKSYTKIEPLVAA
                     CSWKAKRPVKLQLTIEEAMLTTRSDDAYTWMRTAVDATGKIIARQARIYMNTGAYAEN
                     SPLVVEKSSNRCVGPYAIPNVLIENCSLYTNTVPASSYRGFGCAQVTLPGESQIDELA
                     AKIGKDPYEFRLANAAKPGEEFFPGMRPFDGTLKEDLDTAANAIGWKDPLPQGHGRGI
                     ACSGSDAGAYPLTSTAVRVHADGSVTIMTGSTELGQGSHTVIPQIAAEELGVDFDKVN
                     VVSSDTAITPYDRSTGASRTTTLMGSAVFEASREAIQQMVVMAADVLKVKVEDVQVVR
                     GGVRCGEAQLNWSEVISKFYALPDGEVIGRSYIRKSGKFAALPVFWEAAATGVEISVD
                     EETGKITMEKLATVGDVGLAINPALAEGQDIGAATMGMGIGLFEELVYEGSQLMNGSI
                     LDYRLPRFSDLPKKVELHLVQNQDGVGPYGAKGGGEASLNSMAANIANAVERAVGVRI
                     RQAPLTPEKVWRALKEKKG"
     gene            complement(4333..>4613)
                     /locus_tag="VF182_07160"
     CDS             complement(4333..>4613)
                     /locus_tag="VF182_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX6436892.1"
                     /translation="AQGKSVTTIEGLEKPDGTLHPIQQAFIDDFAFQCGFCTPGMIMS
                     AKALLDENPTPSREEIIHHMDGNICRCTGYVPIVAAIERAAQAMSGKK"
BASE COUNT          940 a         1337 c         1271 g         1065 t
ORIGIN      
        1 gatcagcatc atcgagcggg cgccgcattt aaacacagcg aagcttttca ttgaatatgt
       61 gctttccgaa gaggggcagc gcttgctggc aaaacgggga cgggagccgg cacgtccggg
      121 tatcaggccg gaaggatatc cgacaacgct gaaagttgtg ccgagccgag tagagctggc
      181 ggaaaagctt gccgattata caaggcgcta cgaatcgtta ttcgttaaat aatggcgcca
      241 ggcgcgcggt tcaacggttc aaaaattcca cggcgctttc acgctattca acgacagagg
      301 ctttggccca tccgactttg aaccattgaa cgattgaata gcgaagcggt tgaacggaac
      361 aactcaagag ttactcgcta gcccgtccca ccaccggacc ggttttcgcg tggcgttcgg
      421 ttagctcgac aacatagccg tccggatctt tgataaaggc gtaaatatcg ttgccgccgg
      481 gccggttcgg tcccgggcca ctgacaaatt ccacgccctc tgctttgagt ttttcgcaga
      541 gcttgtaaca gtcggaaaca tagagcgcga tgtgacccgt ccactgcgcc agaggagctt
      601 tgcctcgact ttcgacggac tcgatcagtt cgagcgacgg tccttcatcc tcgctgccgt
      661 atcccaaata gcgcacccgt tcgtttcgat caggcacatc gcggatgcgt tggatatcca
      721 tgccgagaag acggctgtag aaatcgacgg tccgatccat gttactcacc ggcaacatgg
      781 tatgtcgaat atggagcgtg ataccatcga tggtgttctg tgggagcatt ttctcctcac
      841 ctcattgtcc ggtggaaccg gctagaagct ttgctagcga actatgatag cgctttgaca
      901 aatgcgcgtt tgaggaaaac cccgatcagg tggcgcttgt agtccgcgcc gccttcgaga
      961 tcatcgaccg gatccgccgc ttgcgccagg gcatccgcgg cgtccgcaag ctgggcttcg
     1021 acttgcgccc gtgacccttc caaaaggcga ttggtttttt ctgattgcgt cggcacggcg
     1081 ctgacactgc cgaccaccgc gctagcttgc ttgatgttct cgccatcaac atcgagcacc
     1141 agcgccaacc ccaatgtcgg ccgttccttg agctggtact ttagataaat ggcgcgttgg
     1201 ctcgtcgtgg gaatcggaat ttcgatcgct tccaggagct catcgcttgc cagcgaagtc
     1261 tcgtacgagc cggtgatcaa cttgtcgatg gtcaacgttc gccgcttcga tttcgcctgc
     1321 agatgcgctt tggcgccgag cgccagtagt aacgtggcag gatcggaatg aggttcggca
     1381 aaacagagat ttccaccgag cgtgccgctg gcgcgcaccc ggacattggc aacattcttt
     1441 tccatttggg ccagcaccgg cagatgctgt tgcacgagcg gcgagcgctc aatggcgcga
     1501 tgagtcgccg tgccgccgat gaagagagcg ccgttttttg actcgatctt gtctagaccc
     1561 gggatcgttt taacgtcgac caggtgctcg tatcgtagca ggtcgtgttt catcgccaga
     1621 agcagctcgg tcccgccggc gtagagccgg cccttttcgc cgaactcgcc gagcatctca
     1681 gaggcttcgg cgacactctt gggttggtac atcgaaaaac gtcttaaggg catcatagca
     1741 gtggccaaga tgaaggatga aagcggaaag ctgaaccgcc gctgagatcc ttcttatctt
     1801 tcgttcatcc tttcttttct ttgagggcgc gccagacttt ttccggagtg agcggggcct
     1861 ggcggatgcg aacgccgacg gcgcgctcga cggcgttggc gatgttggcg gccatggaat
     1921 tcaaagacgc ttctcctcca cctttggcgc cataaggtcc tacgccgtcc tgattctgca
     1981 ccagatgaag ctcgactttt ttcggcaaat cggaaaatcg tggcaggcgg taatccaaaa
     2041 tggatccgtt catcaactga gaaccctcat agaccagttc ttcgaacaag ccgatgccca
     2101 ttcccatggt cgcggcgccg atatcctggc cttcggccaa cgccggattg atggctaagc
     2161 caacatcgcc gaccgtcgcc agtttctcca tggtgatttt gccggtttct tcatcaacag
     2221 aaatttcgac gccggttgct gcagcttccc agaaaaccgg cagagccgcg aacttgcccg
     2281 actttctaat atacgatcgg ccaatgactt caccgtccgg aagcgcatag aacttgctga
     2341 tcacttccga ccaattgagc tgggcttcgc cgcagcgcac gccgccgcga actacctgga
     2401 cgtcttcgac tttcactttg agcacgtcgg cggccatcac caccatctgc tggatcgctt
     2461 cgcggctggc ttcgaaaacg gcgctgccca tcaatgtagt cgtgcggctc gcgccggtgg
     2521 atcgatcata gggagtgatc gcggtgtccg atgaaacaac attgaccttg tcgaaatcga
     2581 cacccaactc ttccgcggca atttgcggga tcaccgtgtg acttccctgg ccgagctcgg
     2641 tgctgccggt catgatcgtg accgaaccat cggcatgcac ccgcaccgcg gttgaggtga
     2701 gcgggtaagc gccggcatcg ctgccggagc aggcaatgcc ccgcccatgc ccctgcggca
     2761 aaggatcttt ccagccaatc gcgtttgccg cggtgtccag atcctctttt aacgtcccat
     2821 cgaaggggcg cattcccgga aaaaactctt cccctggttt ggcggcattg gccaggcgaa
     2881 actcgtaagg atctttaccg attttggcgg cgagctcgtc gatttgcgat tcgccaggca
     2941 aggtcacctg cgcgcagccg aaaccgcgat aggagctcgc cggcacggta ttggtataaa
     3001 gcgagcagtt ctcgatcaag acattcggaa tcgcgtaagg accgacacag cggttgcttg
     3061 acttctcgac cacgagaggg ctgttttccg cataggcacc ggtgttcatg taaatcctcg
     3121 cctgccgcgc gatgattttg ccggtggcat ccactgccgt gcgcatccag gtgtaggcgt
     3181 catcgctgcg cgtggtgagc atcgcttcct cgatcgtcag ctgcagcttc accggtcgct
     3241 tggctttcca tgaacatgcc gctaccagcg gttcgatttt ggtgtatgac ttgctgccgt
     3301 aaccgccgcc gacaaacggc acgatcaaac gcactttgct gaccggcaga ttgaacaccg
     3361 ttttgaggtc gtgccgcacc atgaatggat gctgcgccga cgaataaata ttcaccccct
     3421 gatcggtgac atcggcaacg gcgacgtaag cttccatcgc ataggcatag gtcatcggaa
     3481 aatagtactc agcctcgacc acagcagcgg cgttcttgaa agcctcctcg acgttgcccc
     3541 atctcacttc atgtttctgg caaatgttgc tgcccttgcc cgcggtcact tcgccttcga
     3601 agccacgcag cgcgccggcc tcgaattgtc tttcgtgcag cagcggagca tccttggcca
     3661 atgcctcttg cggcgtgaac accgccggga gctcctggta tttgacatcg atcagctcga
     3721 gcgcttcaaa agcggtgcgc tcatcgacgg cgaccacagc agccaccggc tcaccggcgt
     3781 aacggacttt atcaattgcc agcagcggat ggtccttgac cgcatggccg tagtaagggt
     3841 tcaacccctc gagatcatta ccggtgacca cgcagaaaac tcccgggtgc gcccgcgccc
     3901 gcgatgtatc aatggagata atcttggcgt gcgcataagg gctgcgcagc actttggcgt
     3961 aagccatgcc gatgagcttc aagtcggcca cgtactgggc cttgccggtg acctttacac
     4021 ggccgtcgcg ccgcggcaac gaatgattga cgacatgaaa atcagttttc ttctctgcgc
     4081 tcacttcttg gactccttgc tcatcacctg agcggcacgt ttaatgactg attgattgac
     4141 catgtagaaa gattgtggcg ttcacttctt cgtctccctg ctcgggtctg ggcggcgcgt
     4201 tcaatgagta aatgatagac aacgtaaaat cgattttctt ctctgctcac ttcttggact
     4261 ctttgctcct agcctgggcg gccggttcaa tgagtaaatg acagccacgc agaagcggtt
     4321 ttctttttgc gcttacttct tgccgctcat cgcttgggcg gcgcgttcaa tagcggcaac
     4381 gataggaacg taacccgtgc agcggcagat atttccgtcc atatgatgaa tgatttcctc
     4441 gcggctgggt gtcgggtttt catcgagcag cgctttcgct gacatgatca tgcccggcgt
     4501 gcaaaaaccg cattggaaag caaaatcgtc gatgaacgcc tgctgaatcg ggtggagcgt
     4561 gccgtcgggt ttttccaagc cttcgattgt cgtgacgctt ttaccttgtg ctt
//
LOCUS       DASUVS010000127         3255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1576041, whole genome shotgun sequence.
ACCESSION   DASUVS010000127 DASUVS010000000
VERSION     DASUVS010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576041"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VF182_07165"
     CDS             complement(<1..347)
                     /locus_tag="VF182_07165"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436893.1"
                     /translation="MLFKFFARSFSLVMCMGISSWLAHGQETVTVALTSKAFQYTILP
                     IAQDRGYLKEEGIDLKLVYMQNAPGLQALTAGNVQFSGSGSSALVAISKGGAPFKTVI
                     AANDQVLQWVVVRP"
     gene            571..1542
                     /locus_tag="VF182_07170"
     CDS             571..1542
                     /locus_tag="VF182_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6436894.1"
                     /translation="MKAIRIHNFGPTEDVLQFEEAPVPEPGSGELLIKVEAASLNRAD
                     LGLRSGNYRIAQEDLPIIPGREFAGRVTKLGDGVSDFQVGQRVVAYTGTGGYAEYAKA
                     KVSEVRPLPDGVDAAAAAALPTVFLTAWFALMEEGKLKSGEWLLVQAGSSGVGTAAIQ
                     IGKDVGAKVVTTTSGDEKARRLGQLGADAVIDYTQTDLVAEGRRATGNRGFDVVLEMI
                     GGEVYQKSIEVLAPAGRLVSIGGAFGKIPDTPPALSEGRTASRFSITNYLKAHPEGFK
                     QLDAALHWVRDGKYQVVIDRRFPLAETRAAQRFLEGRDHFGKILLIP"
     gene            complement(1563..2111)
                     /locus_tag="VF182_07175"
     CDS             complement(1563..2111)
                     /locus_tag="VF182_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436895.1"
                     /translation="MEDERELGWLRLTDKSQLVLTTGHVFQHQDYGMFGENRAIIPRK
                     AITTVRLSWRRSRGALVLGLILFLASLAMIIANQTNQPEGITLNTRLFAVSPSTLSWI
                     QYGLLIAGLALIALFWFHKRNEIQILGGTGSIGGTPVSYEDAEEFCLLLMGAGKKAAP
                     TIANVEPEPENSPKTTDPDWRL"
     gene            complement(2201..>3255)
                     /locus_tag="VF182_07180"
     CDS             complement(2201..>3255)
                     /locus_tag="VF182_07180"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6436896.1"
                     /translation="MTKVKGFIDADGHVVENDRALLDFLPPPYKGREDLLSFPFFPTL
                     DGFHRQALRVVDGKGRYVAQGSLKEWKDFLDEDEIEWSVLYPTAGLTFGLIRDKDWAC
                     AIAQGYNNYLSQVFLKQEKRLRGMALIPLQSIPEAVKELRRAVKELNMVGAILPAVGM
                     RQPFGDERYFPVYEAAQELEALLAVHAAPTQGIGVDAFDRLIEVRTLSHGFGQMIQMT
                     GMIYGGVFDKFPRLKVAFAEAGCGWVPYLMERMDLEYEHRSAQVPELKITPSEHLKSG
                     RIFIHCELEELGIPRIVDLLRDDILFCATDFPHEPRSEFRENVSKFVAREDLAMETKQ
                     KIMTQNPKRMYPLAAN"
BASE COUNT          764 a          906 c          863 g          722 t
ORIGIN      
        1 ggccgcacga ccacccattg cagaacctga tcattcgcag cgataacagt tttgaaaggc
       61 gcgccgcctt tggaaattgc caccagcgcg ctgctacccg agccagaaaa ttgcacgttg
      121 ccggcagtca acgcctgtag acccggcgcg ttttgcatgt aaacgagctt aagatcgatg
      181 ccctcttcct tcaggtatcc gcgatcctga gcgatcggca aaatcgtata ctgaaaagct
      241 ttcgacgtta gtgccacggt aacggtctcc tgaccgtgtg caagccagga cgaaatgccc
      301 atgcacatca ccaaagaaaa cgagcgcgcg aaaaacttaa acaacattga aatgcacctc
      361 ctatgacgtt gaccaagtca acgtcgatgc ttatcgcttt aaaagcgcga acctttatcc
      421 gtcaaagaaa tgcgccctaa cctactatca ggtcattgtt tccgtcaaat tgaaagactc
      481 cgaaaagact ctgttctgct acgcattgac atgagagctc aggcttggat aggctgactg
      541 cagaatgaca gggtaatgga aggagctgca atgaaagcca ttcgcatcca caatttcgga
      601 ccaaccgaag acgtgctcca gttcgaagaa gcgccggtgc cggagccggg ctctggcgag
      661 ctactgatca aagtcgaagc cgcttcgctt aatcgagccg atcttggctt gcgcagcggc
      721 aattaccgca tagctcagga agatctgccg atcattcccg gccgcgaatt tgcagggcga
      781 gtgaccaaac tgggcgacgg tgtgagtgac ttccaagtcg ggcagcgagt cgtcgcctac
      841 accggcaccg gcggatatgc cgaatacgcc aaagcgaaag tatctgaagt gcggccgctg
      901 ccggatggcg tggacgccgc cgccgcagca gcgcttccga cagttttttt aactgcctgg
      961 ttcgctctga tggaagaagg caaacttaaa tcgggcgagt ggctgctggt gcaagccggc
     1021 agcagcggcg tcggtactgc ggcgattcaa attggcaaag acgtgggcgc taaagtagtt
     1081 acaacgacca gtggcgatga gaaagcgcgc aggctggggc aacttggcgc ggatgccgtg
     1141 atcgattaca cccaaactga tttggttgcg gaaggtaggc gcgccacagg caatcgcggc
     1201 tttgatgtcg tgctcgaaat gatcggcggc gaggtttatc agaaaagcat cgaagttctt
     1261 gcgcccgccg gccggctggt ttctatcggc ggcgcctttg gcaaaatccc ggacacgcct
     1321 cccgctctta gcgaaggacg aaccgccagc cgcttttcga ttacgaatta tttgaaggcg
     1381 catcccgaag ggttcaagca gcttgacgct gcgctacact gggtccgtga tggaaaatat
     1441 caggtggtca tcgatcgtag atttcccttg gcggaaacgc gcgcggcgca acgttttctc
     1501 gagggacggg atcacttcgg taagatcctc ttgattcctt aagcccgaac caaaccacct
     1561 ctctagaggc gccaatcagg atcggtcgtc ttcgggctgt tctccggctc aggttccaca
     1621 ttggcaatcg ttggcgctgc ttttttgccg gcgcccatga gaagcaagca gaactcttca
     1681 gcgtcttcgt aactcacagg tgttccgccg atgctaccgg tgccaccgag aatctgaatt
     1741 tcatttctct tgtgaaacca gaacagtgca atcagggcta ggccggcaat cagcagcccg
     1801 tattgaatcc atgacaaggt cgatggcgag acggcaaaca gccgggtgtt aagagtgatc
     1861 ccctccggct gattcgtctg attggcaatg atcattgcca gggaagcaag aaataagatc
     1921 agacccagaa cgagcgcccc cctgcttcgt cgccagctta gacgaaccgt ggtgatcgcc
     1981 ttgcgcggga tgattgctcg gttctcgccg aacataccgt aatcctgatg ttggaaaacg
     2041 tggccggtcg tcaacaccaa ctggcttttg tcggtcaacc gcaaccagcc gagttccctc
     2101 tcatcttcca tcgtttctcc gtcaggcgag ttcgacctct gcctcttgca tgattatcgg
     2161 tgaccttttc tgtgacgcaa caaaccagga atcggccgtt ttaatttgcc gccagtggat
     2221 acattcgttt gggattttgc gtcatgatct tctgcttggt ttccatcgcg aggtcctcac
     2281 gagcgacaaa tttgctgaca ttttcgcgaa actccgagcg tggttcgtgg ggaaaatcag
     2341 tcgcgcagaa caggatatcg tcgcgtaaca ggtcgacgat tctggggatg ccgagttcct
     2401 cgagttcaca atggatgaag atacggccgc ttttgagatg ttcggaggga gtgatcttta
     2461 gctcggggac ctgcgcgctc cggtgttcgt attccaggtc catgcgttcc atcagatacg
     2521 gcacccagcc gcaaccagcc tcggcaaagg cgacttttaa tctgggaaat ttgtcgaaaa
     2581 caccgccata gatcatcccg gtcatttgaa tcatctgtcc aaagccgtga ctcaaggtcc
     2641 gaacctcgat gagacggtcg aaggcatcca cgccgatgcc ttgagtgggc gccgcatgaa
     2701 ccgccaacag cgcctccaac tcttgggccg cttcatagac tggaaaatat ctctcgtcac
     2761 cgaagggctg cctcattccg actgccggga gaatcgcgcc gaccatgttg agttccttga
     2821 ccgctcgccg taactccttt acggcctccg gtatggattg cagcggaatc agcgccatgc
     2881 cacgaagccg cttctcctgc tttaaaaata cttgcgacaa ataattgtta tagccttggg
     2941 ctatcgcgca ggcccaatcc ttgtctcgga tcaaaccgaa ggtcagtccg gcggtgggat
     3001 acagtaccga ccattcgatc tcatcttcat ctagaaaatc cttccattct ttgagcgatc
     3061 cctgtgccac atagcgacct ttgccatcca cgacgcgcaa agcctgacgg tggaatccat
     3121 ccaaggttgg gaagaacggg aacgacaaga gatcctccct gcccttataa ggcggcggca
     3181 agaaatccaa aagagcccga tcattttcga ctacatgtcc gtcagcatcg ataaatccct
     3241 taaccttagt catcg
//
LOCUS       DASUVS010000128        22397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_47155, whole genome shotgun sequence.
ACCESSION   DASUVS010000128 DASUVS010000000
VERSION     DASUVS010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 22397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22397
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47155"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VF182_07185"
     CDS             complement(<1..217)
                     /locus_tag="VF182_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX6436897.1"
                     /translation="MNRTTIWNPETYARDARFVANLGQPLLELLAPKNGEVIMDLGCG
                     DGALTEQIAQSVTVLATDASLAQARAAK"
     gene            289..1485
                     /locus_tag="VF182_07190"
     CDS             289..1485
                     /locus_tag="VF182_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX6436898.1"
                     /translation="MATKDADLAALRIDRSFQRGDRPRKSRIKPKILIGAIVALVLAA
                     TIMFFKGLFDPAVEVQLATVALTSPSQTNSILTASGYVVARRKAAVASKGTGRLVLLN
                     VEEGDQVKKGQVIARLEDADVVAARERARQSLLLAEADLYDAKTSYERLRTLLKQAAI
                     AQADYDVAEARYKRVIASIDVARFALKEAEVAVENTRIIAPFDGTVLKKNADVGEIVA
                     PLAGAASSKAAVVTIADMSSLEVDADVSEANITRVAPEQQCEITLDAYPQQRYPGYVT
                     KIVPTADRTKATVLVKIKFKEYDQRVLPEMSAKISFLAIGYKADSADYKLLLTVPETA
                     VASRAGRAVVYQIRDDRAVEVPVSTGQKLGGLIEIKSGITEGEKIIAKADDRIATGAK
                     VAVKGP"
     gene            1502..2176
                     /locus_tag="VF182_07195"
     CDS             1502..2176
                     /locus_tag="VF182_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6436899.1"
                     /translation="METPLIEVVDLFKSYQRDSLEIPVLRNISLEIAEGDFLAFMGPS
                     GSGKTTLLNLIAGIDKPTSGKILIGEIDITELSETELAVWRSQNIGFIFQFYNLIPVL
                     TALENVELPLVLTPLSRKERRSHAATALEVVGLGERMHHYPRQLSGGQEQRVAIARAI
                     ATDPAILVADEPTGDLDKISAEEVLELMHRLNRELNKTILMVTHDPRAAERARSVRNL
                     DKGELA"
     gene            2185..3345
                     /locus_tag="VF182_07200"
     CDS             2185..3345
                     /locus_tag="VF182_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX6436900.1"
                     /translation="MELLKLLLRNTLRHKLRSFLTLTGIGVAVMAFGLLRTVVTAWHA
                     GVEASAANRLITRHAVSFVFPLPLAYRDRIAQVRGVNKVTYANWFSGVYIDKNQFFAR
                     LAVDSETFFDVYPEFIIEREQFEAFKRERNACIIGIDIARRYGLKLGDIMLIEGDVYP
                     GQWEFVVRGIYQPKDQTTDPASMMFHYQYLEERVRQDMPQRSGEIGWYVVRIDDPDSS
                     AAISQEIDQLFANSRAETKTETERAFQQSFLSAASAVITAMNIMSFVIIGIILLVLAN
                     TMVMSARERTHEFAVLKALGFSGGQLFFLIAGEALILSTLGSALGLLVTVPAVEGFQT
                     ALPKGWFPVFYIKPETIILGCVSAFVVGLAASLIPLRRVLTTRIVEGLRHVG"
     gene            3346..4515
                     /locus_tag="VF182_07205"
     CDS             3346..4515
                     /locus_tag="VF182_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6436901.1"
                     /translation="MRIPLKYILRSSSSRRLTTVITILGIALVVFVFTAVLMMANGVE
                     KTLRSTGSDDNLIVVRKAAMSEIMSILDRDAASIVSNMPQIASFADGRPMSSKEAVVI
                     INLNKIGAPGISNVIVRGVEPAAFPLRPQVKMVQGRMFRWGAREVIVGAGITHRFEGA
                     QIGEKMKFGGDLWTVVGIFEAAGSGFESEAWGDSNQIMDAFKRSSFSTVTMRLKQPDD
                     LSEIAAAFESDNRLQYFAAKREKKFFEEQSEMMAKFIRILGIFVTVIFSTGATIGAMI
                     TMYGAVANRTVEIGTLRALGFFRRSILLAFLGESFVLSLAGGIVGLGLASVLQFFSIS
                     TLNFGSFSELAFSFALSPSIIITSLGFSLLMGLIGGFLPALRAARLNIIQALRAV"
     gene            complement(4745..5836)
                     /locus_tag="VF182_07210"
     CDS             complement(4745..5836)
                     /locus_tag="VF182_07210"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX6436902.1"
                     /translation="MENSLPQEQALQPGKRDPLANLATSKLKRFEPTWHKTIAAYGSS
                     NLRKALGQLLNTFIPYCLLWALMLQTVQRGYPYWITLALALVAGGMMVRIFILFHDCC
                     HGSFFPSRTANTILGYVSGIFTFTPFEDWKYGHNSHHATAGDLDRRGIGDIWTMTKEE
                     YLAASARKRLRYRFYRNPFVLFGPGAALLFLFIQRFPTKGAGKRERRSVLFTNLALLA
                     IVAVAGFTIGLKTYLLIQLPIILVGGTLGLLLFYVQHQFENVYWARHEAWNPLRVALE
                     GSSYLKLPKVLQWFTGNIGLHHIHHVRPNIPNYNLQRCYDEIPAFQEVKPITLWKSFS
                     LLRLGLYDEAQRKMISFRSLKVTPLDSPA"
     gene            complement(5930..6157)
                     /locus_tag="VF182_07215"
     CDS             complement(5930..6157)
                     /locus_tag="VF182_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436903.1"
                     /translation="MKKLMLLSVLIVGLAQSGCEFISGALVGAGAAGGGYEYQAYRQM
                     QQLEEDYKNKRISREEYDDRKKQIEKGSIIY"
     gene            complement(6218..7027)
                     /locus_tag="VF182_07220"
     CDS             complement(6218..7027)
                     /locus_tag="VF182_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6436904.1"
                     /translation="MERTGEENDLPNKSSASGTKHRTGGVTHLRAIAGGLQPHHKQSQ
                     QRAQVRRFTALLNTTTALFAHEVASPLQGMSASLELIERDLKTNHDPLLTTTMRSFRR
                     EVDRLIILLEEFRSASVPQNLNLTGSDLRQVVEDALAIQLVVYQTAGVNLKLEFKDGL
                     PAVKLDTAKIKQVILNLCSNALDAMPRGGELTIRGYSLGATVVLEIADTGIGMPEGID
                     PFALFQTTKVHGSGLGLPIVEQIVLAHNGTIDHTSEAGRGTTFKIVFPAAV"
     gene            8092..11088
                     /locus_tag="VF182_07225"
     CDS             8092..11088
                     /locus_tag="VF182_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX6436905.1"
                     /translation="MTMVARKSEKIFAQKAARPASRKLAKGRRTSDSAAVSEDRKDGF
                     HIVGIGASAGGLKALESFFSAMPVNCGMSFIVISHLDPKHSSMLPDLLKRYTKIMVDR
                     AQNGIRVERDRIYVIPPNTEMTLVRGTLVLKKPSEPHGQRFPIDTFFRSLARDRKQRA
                     ICIILSGTGTDGTSGLRDVKAEGGMAMAQATASAEYDGMPRSAIATGLVDYSLPAEKM
                     PAQLIKYVSDRHLKGDAGTHVEQPAESMEELFHLLESQTGHDFSSYKKSTISRRFNRR
                     MGVQRIKSLSSYLGFIRRNPSEIDALFKELLIGVTHFFRDPEAFKSLSKVLSATLRKH
                     PKDSTFRIWSPGCSTGEEPYSLAILLDECRSKLKKRFRVQIFATDVDSEAISLARAGV
                     YSGSVVSGLTAERLKRFFLKDNGVYRVKKEIRDMVVFAEQDLIRDPAFSKLDLISCRN
                     LLIYLETELQKQLLRLFHYALKPGGLLFLGTSESIGIFSHLFSPVDKKSKIFRRRNGG
                     AFANEAVGEFLVRPLTHARPDRAPGRPGKKKDDNGPVITNLTEKKLLEEYTSPSVLIN
                     QNGDIVYAFGRTGKYLELPQGHAALNIHDMAHPGIKDALAAAIHEAAIRNKRVVVEGL
                     ILSPEAELNRVKISIEPLQVKPEGSVGMMVVTFEDIGVEMKDGTPKRRGAGYGTQLER
                     ELKLARQRLKRSIEDLEVSNEELKSANEESQSTNEELQSTNEELETSKEELQSINEEL
                     VTVNAELQGKIEELTVVNDDVRNLLDNTNIATIFLDKQLRIKRFTPAATKIVNLIPTD
                     IGRPLNHLVFNLELPVHAEAKKVLETLIPNEMEVRNNEGRSYLSRIMPYRTVDDLVDG
                     VVITFTDITESKRALVQQEMLDYLKSIVATIRDSLVVLDGDLKVITASRSFYQTFQVQ
                     PESTEGVLLYELGDGQWNIPSLRELLEKILTTNASFEDFPVEHDFPGIGHKKMLLNAR
                     KVYRENIDTGRILLAIEDTTGAVSAS"
     gene            11123..12733
                     /locus_tag="VF182_07230"
     CDS             11123..12733
                     /locus_tag="VF182_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6436906.1"
                     /translation="MREKDKEEVNLRKRAEDLLKKTGKSTPRIVHQRAAEVIHELDVH
                     RVELEMQNEELRRSQVSAEESQRKYAELYEFAPIGFCSFDRKGQIQQVNLACAQMLRI
                     EKRYLHKRWFRSFVSPGFRDEFDRFFHKLFQPGHRESCESQLTRHDGALIDVELVGGA
                     VADPALGVAHESQIAILDISQRKKAESWSRRLVETTQDAVLSIDRKGRIVLFNAAAEQ
                     TFGYSRNEIAGQKVNVLMSEPYVSQHDEYIARYERTGEAHAIGRIRTVTAKRKNGDLF
                     PIELSVTEIPTDEDVHYAAFIRDISDKTRLQEQLLESERLAAIGATAAKIAHEIANPL
                     NNIYMTTQVLERELAKEEIPVHGQLASDLKTITSETARLNALLQQFRMISRKETYDFH
                     PVSIAALIKEVADTCRAIWADQKIHFDHVFEPNLPSISADRDKLKQVLLNLYKNGAEA
                     MPGGGKITVKASSTETGVLVEITDTGTGIPPDMDIFEPFATTKLGGTGIGMVVVRQVV
                     TAHGGSITYHSEPGKGTSFFIALPALKS"
     gene            complement(12761..13441)
                     /locus_tag="VF182_07235"
     CDS             complement(12761..13441)
                     /locus_tag="VF182_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX6436907.1"
                     /translation="MIQDNPEIDKFKQQLRDSLQNETQNSRSVKVPRHENVYNSGDGD
                     GMIYFIETGQVKLLMLSSEGKECILGIYTAGDIFGELCLSGLGERLETATAMKATSLK
                     QIPSIQFFARLGRDSLWQGFVRYLAVRIADQQQIIANLVTVDSEQRLGQTLLQLARTM
                     GKKDPRSIRIELRITHEELSEMVGTTRPRISLFMQRFHNLGLIETNKDRFLIIKESKL
                     TDYLAQIA"
     gene            complement(13497..13922)
                     /locus_tag="VF182_07240"
     CDS             complement(13497..13922)
                     /locus_tag="VF182_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436908.1"
                     /translation="MYSKRAPASPKHRRSEARQRHGLHDRRSNIKALPEQFFNTRISL
                     ASVCPETALMYAVLEDALLCFQEKVAGSPHQQRCRAQEAARWFFSGDQHSPYSFVSIC
                     AWLGLDAHDIRNKLKYLEQDIRGKRYLAKRRSIARNNDS"
     gene            complement(13987..15444)
                     /gene="sufB"
                     /locus_tag="VF182_07245"
     CDS             complement(13987..15444)
                     /gene="sufB"
                     /locus_tag="VF182_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX6436909.1"
                     /translation="MNTSTQTTEDLVNQEYKYGFVTEIETDTVPRGLNEATVRLISAK
                     KNEPSFMLDWRLKAYRHWVKLAKSEAEPKWANVHYPAIDYQDLIYYSAPKTNVGLKSL
                     EEVDPKLLETYDKLGIPLLEQKWLTGVAVDAVFDSVSVATTFKEKLASLGVIFCSFSE
                     AVQKHPVIVRKYLGSVVPYSDNFFAALNAAVFSDGSFCYIPRGVRCPMELSTYFRINA
                     AETGQFERTLIIADEGSSVSYLEGCTAPMRDENQLHAAVVELVALDNAQIKYSTVQNW
                     YPGDKDGKGGIYNFVTKRGKCLGNNSKISWTQVETGSAITWKYPSCILQGDNSVGEFY
                     SVALTNNYQQADTGTKMIHIGKNTKSTIISKGISAGHGQNTYRGLVKVMKGAAGARNY
                     SQCDSLLLGDKCGAHTFPYLEVMNNSSQVEHEASTSRIGEDQLFYCRQRGISPEDAVT
                     LIVNGFCKQVFRELPMEFAVEAQKLLGVSLEGSVG"
     gene            complement(15694..16035)
                     /locus_tag="VF182_07250"
     CDS             complement(15694..16035)
                     /locus_tag="VF182_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX6436910.1"
                     /translation="MKNQNRLGENIEFDERANEDKYFALKEHERIDEMKVDFQNAEAA
                     RRERQVGNCPMCAGKLEKYGFMGFVLQRCDRCQGIWLKPAELHGIMRKAARGPLGAFF
                     DRCFAKIEQIK"
     gene            complement(16136..16462)
                     /locus_tag="VF182_07255"
     CDS             complement(16136..16462)
                     /locus_tag="VF182_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006471759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX6436911.1"
                     /translation="MVGEITLSDRAAEKIRRLLGSETDEKGLRLKVVAGGCSGFEYKM
                     AIDAIAADDRVIERNGARILLDPTSAIHLNGTELDYKDDLMQSGFIFINPNVKSTCGC
                     GTSFSA"
     gene            complement(16481..16969)
                     /gene="nuoB"
                     /locus_tag="VF182_07260"
     CDS             complement(16481..16969)
                     /gene="nuoB"
                     /locus_tag="VF182_07260"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX6436912.1"
                     /translation="MPENVLTTKVDDLLNWGRASSQWYMLFGLACCAIELMQTGGPRA
                     DVDRFGAVPRATPRQSDLMIVAGTLTYKMAKRTKLLYDQMPDPKYVIAMGSCASCGGL
                     FQLAYSVCKGVDKIVPVDVYVPGCPPRPEALTEGLLRIQDKIMREKWLVKKTRQEPEL
                     AI"
     gene            17441..17884
                     /locus_tag="VF182_07265"
     CDS             17441..17884
                     /locus_tag="VF182_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6436913.1"
                     /translation="MKKYKKIPQVGSVMTPFPYSVDTDDTIAEIERLMDEHQIRHVPV
                     QQDGRVVGIISERDVYRLLERFLPQAQKEQIRARDIMIADPYVVSFEAPLNEVALEMA
                     KRHIGSVIVLHHEKLAGIVSATDICRILAEIFETEFAAATGDDVA"
     gene            17945..18133
                     /locus_tag="VF182_07270"
     CDS             17945..18133
                     /locus_tag="VF182_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436914.1"
                     /translation="MGNQTYQKRQKELARSQKKKDKAARRMQRKTEKVKGGPPLEDDP
                     MTGGRMTSESSLQSSPAV"
     gene            18468..18782
                     /locus_tag="VF182_07275"
     CDS             18468..18782
                     /locus_tag="VF182_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX6436915.1"
                     /translation="MGSKLYVGGLPYSVTEGRLQEVFSAHGTVESANVISDKFTGQSR
                     GFGFVEMGSSGEAQRAISAMNGTQLDGRTLTVNEAKPQARRDGGGDREGRGNRDGGRN
                     RW"
     gene            19169..21457
                     /locus_tag="VF182_07280"
     CDS             19169..21457
                     /locus_tag="VF182_07280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6436916.1"
                     /translation="MQGSSSPSAIDRIAFIGNYLPRQCGIATFTTDLCEAIAAEHDDT
                     SCIAVPVNDIESGYAYPSRVRFELVEKDIESYRRAADFLNINSVDLVCLQFEYGIFGG
                     RAGSHILALLRELRIPIVTTLHTILREPDPHQRRVLEEVAALSDRLVVMSQRGAEFLE
                     TIYQISPYKIDLIPHGIPDVPFVDPSFHKDVFGVEGKTVLLSFGLLSANKGIENVIAA
                     LPAILAKHPNVVYFIVGATHPHVKRQDGETYRLSLQRLALDRGVAGHVIFYNRFVSME
                     ELVEFIGATDIYITPYLNPAQIVSGTLAYTVGAGKAVISTPYWHAEEMLAEQRGALVP
                     FRDPVALADQVIDLLSNDAKRNAMRKRAYMYGREAIWPQVARRYMETFQRARAERRHF
                     APSDFAVKPLDKRPGEFPPLKLDHLQRMTDHTGMFQHAIFTVPNYLEGYTIDDNARAL
                     LVSALLEELGNSEAYELSSRYLAFVWYAFNSETRRFRNFMDFQRNWLEESGSDDSHGR
                     TLWALGTLLGRSSTPALQSTAGRLFEQALPLISDTTSPRAWAFALIGIHEYLRRFDGD
                     RRASQVREQLAGRLLALFEKNRAGDWRWYESSLTYCNAALPHALLMSGQRIPNYKMIE
                     SGLESLQWLAELQRADTNGGHFVPIGSNGFYQRGGERARFDQQPVEAQAMVSACLEAY
                     RVTGDKGWHNEARRAFDWFLGRNDLNLPIYDPVTGGCRDGLHSDRANENQGAESSLAY
                     LQAVLEMRLAETTLLSVETGAR"
     gene            21454..>22397
                     /locus_tag="VF182_07285"
     CDS             21454..>22397
                     /locus_tag="VF182_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HEX6436917.1"
                     /translation="MTNQHQELFRRQKTNPILSANNWPYPINSVFNPGATLLPDGTTL
                     LLCRVEDRRGHSHLTVARSTNGVDNWQIESQPTFRADPKQFPEELWGIEDPRITYIPE
                     LSKYAIVYTAFTRDGPGVSLALTQDFREFERYGVIMPPEDKDAALLPYRIGGQWALIH
                     RPVSAPRAHMWLSYSPDLRHWGNHKLMLEARRGAWWDANKIGLSPPPIETPQGWLVIY
                     HGVRNTASGAIYRLGLALFDLDAPEHCLKRGDEWIFGPQATYEQRGDVDNVVFPCGYT
                     LESDGDTLRLYYGAADTSIGLATGSLRRMLEWLENHQS"
BASE COUNT         5181 a         5887 c         6071 g         5258 t
ORIGIN      
        1 ctttggccgc gcgcgcctgg gcaagactgg catcggtggc gaggaccgtg actgactgag
       61 cgatttgttc cgtcaaagcg ccgtcgccac aacctagatc catgataacc tcgccgtttt
      121 tgggcgcaag cagttctaaa agcggctgac ccagatttgc gacgaagcgc gcatccctgg
      181 cataagtctc ggggttccag attgtcgtgc ggttcatcta gcgattttct aacagcttcc
      241 gctcgggttg aaaacgatga ttcgatcggc ttactgtaaa tgtttaaaat ggctacgaaa
      301 gacgctgatc tggcagcatt gagaattgat cgctcgtttc agcgtggcga tcggccgcgt
      361 aaatcgcgaa tcaaaccgaa aattctgatt ggtgcgattg tcgctcttgt gttggcggca
      421 acgatcatgt ttttcaaagg tctgttcgat ccggccgtcg aagtccagct ggcgacggtg
      481 gcgctaacct caccgtcaca gactaattcg atattgaccg ccagcggtta cgtcgtggcc
      541 cgccgcaagg cagcggtcgc ttccaaaggt accggacgtt tagtgctcct caatgtagaa
      601 gaaggtgatc aggtcaaaaa gggccaggtg atcgcccggc tcgaagatgc cgacgtggtt
      661 gccgcccgtg agcgcgctcg acaaagcctg ctccttgcgg aagccgacct ctacgatgca
      721 aagacgtctt acgagcgcct gcgtacacta cttaaacagg cggcgatagc ccaggccgac
      781 tacgacgtcg ccgaggcgcg ttacaaacga gtcattgcat ctatagatgt cgcgagattc
      841 gccctgaagg aagcggaagt cgctgttgaa aatacccgga tcattgcgcc cttcgatggc
      901 actgttctga agaagaatgc cgatgtcggt gaaattgtcg cgccgctggc gggcgccgcc
      961 agttcgaagg cggcggtggt gacgatcgct gacatgtctt ctctggaagt cgatgccgac
     1021 gtgtccgaag ccaacattac cagggtggcg ccggaacagc agtgtgaaat tactctcgac
     1081 gcctatccgc agcagcgata ccccggatac gtcacgaaga ttgttcctac cgccgatcga
     1141 accaaagcga ccgttttggt taagatcaag ttcaaggaat atgaccagcg ggttctgccg
     1201 gaaatgagcg caaaaatcag ctttctcgcg atcggctaca aggcggacag cgccgattac
     1261 aagctgctgc tcacggtgcc cgaaacggca gtggccagtc gcgccggccg cgcggttgtc
     1321 tatcaaatca gagacgatcg cgccgttgaa gtgccggtga gcacaggtca gaagctcggc
     1381 ggcctgattg agatcaagag cggcattaca gagggagaga agatcattgc caaggctgat
     1441 gatcggatcg caacgggagc aaaagttgcc gtcaagggac cctaactctt gccatcgcag
     1501 catggaaact ccgctcatcg aagttgtcga tctgttcaaa tcctaccaac gtgacagctt
     1561 ggagattccg gttctgcgta atatttctct cgaaattgca gagggagact tccttgcctt
     1621 tatggggccg tccggctcgg gtaaaaccac tctgttgaat ctgatcgccg gcatcgataa
     1681 gccaacctcg gggaaaatct tgatcggcga gatcgatatc acggaattaa gcgaaaccga
     1741 gctcgcggtg tggcgctcgc agaatatcgg atttatcttt cagttctaca acctcatacc
     1801 ggtgctcact gcgttggaaa atgtcgagct gccgctggtt ctgaccccgc tctcgaggaa
     1861 ggagcgccgc agtcatgccg caacggcatt ggaggttgtc ggcctcggcg agcgtatgca
     1921 tcactatcca cgccaacttt cgggcggcca ggaacagcgg gtcgccatcg ctcgagcgat
     1981 cgcgacggac cccgcgatcc tggttgccga tgagccgacc ggcgatctcg acaagatctc
     2041 cgccgaagag gtgctggaac tgatgcaccg attaaaccgc gagcttaaca agacgattct
     2101 tatggtcacc cacgacccgc gcgccgccga gcgcgcgcgc tcggtacgaa acttggacaa
     2161 gggcgagttg gcgtaggaaa gatcgtggaa ctgctgaagc ttctcctgcg caacacgttg
     2221 cgtcacaagc tacgaagttt cctgacgttg accgggattg gcgtcgccgt catggcgttt
     2281 gggctactgc gaaccgtggt gacggcgtgg cacgccgggg tcgaagcatc ggcggcaaat
     2341 cgattgatca cccggcacgc cgtgtcattc gtgtttcctc tgccgctggc gtatcgagac
     2401 cgcattgctc aggtgcgcgg cgtcaacaaa gtcacttacg cgaattggtt ctccggcgtg
     2461 tacatcgaca agaatcagtt tttcgctcgt ttggcggtgg actccgagac gtttttcgat
     2521 gtttatcccg agttcattat cgaacgcgaa caattcgaag ctttcaaacg agagcgcaat
     2581 gcctgcatca tcggcatcga tatcgcgcgc cgctatggtc tcaagctcgg cgatatcatg
     2641 ctgattgagg gagatgttta tcccgggcag tgggagttcg tcgtgcgcgg catttaccag
     2701 ccgaaggatc agaccaccga tcccgcgagc atgatgtttc attatcaata tctggaggaa
     2761 cgggtgcgcc aggacatgcc gcagcgctcc ggagagatcg gttggtacgt tgtgcgcatc
     2821 gatgatccgg acagctccgc ggcgatctcg caagagatcg atcagttgtt cgccaattcc
     2881 cgagccgaga ccaagaccga gaccgagcga gcctttcagc agagtttttt atcggcagcc
     2941 agtgccgtca ttacggccat gaatatcatg tctttcgtca ttatcggaat tatcctgctg
     3001 gtcctggcca ataccatggt aatgtccgcg cgcgaacgca cgcacgaatt cgcggtgctc
     3061 aaggcccttg ggttttccgg cggccagttg tttttcctga ttgccggcga agctttgata
     3121 ctttcgacgc tcgggagcgc tctgggactg ctagtgaccg tgccggcggt ggaaggattt
     3181 caaacggcgc tgcctaaagg ctggtttccg gttttttaca tcaagcccga gaccatcatc
     3241 ctgggttgtg tttcggcctt cgtcgtcggc ctagcggcct cgctgatacc tctgcggcgg
     3301 gttctgacca cccgcattgt cgaaggtctt aggcatgtcg gatagatgcg gattccgctg
     3361 aaatacattc tgcgcagttc ttcctcgcgc cgcttgacca cggtgatcac gatactcggc
     3421 atcgccttgg tggtgttcgt tttcaccgcc gtcttgatga tggccaacgg cgtcgagaag
     3481 acacttcgct ccaccggttc ggatgataac ctgattgtcg tgcgcaaggc ggccatgagt
     3541 gaaatcatga gcatcctcga tcgagacgct gcgagtatcg tcagtaacat gccccagatc
     3601 gcgagctttg ccgacgggcg gccgatgagc tccaaagagg cggtggtgat catcaatctc
     3661 aacaagatcg gcgcgccggg aatcagcaac gtcatcgtgc gcggcgtcga accggccgcg
     3721 tttccactgc ggccccaggt caaaatggtt cagggccgaa tgtttcgctg gggcgcacgt
     3781 gaagtcattg tcggcgccgg catcacgcat cgattcgaag gcgcgcagat cggcgagaag
     3841 atgaaatttg gcggcgatct ctggacggtg gtgggtatct ttgaagcggc cggcagtggt
     3901 tttgagtccg aggcctgggg tgacagcaat caaattatgg atgcgttcaa gcgctcatca
     3961 ttttcgacgg tgacgatgag actaaaacaa cccgacgatc tttcggagat tgcggcggca
     4021 ttcgaatcgg acaatcgctt gcaatatttt gccgccaaac gggagaagaa attcttcgaa
     4081 gaacaatcgg agatgatggc caagttcatt cgaatcctcg gtatctttgt gaccgttatt
     4141 ttcagcactg gcgcgaccat cggcgcgatg atcactatgt atggcgcggt agctaatcga
     4201 acggtggaaa tcggcacgtt gcgggcctta ggtttttttc ggcgcagcat cctgctggcc
     4261 ttcttgggcg agtctttcgt attgtctctg gcaggcggaa tagtgggact aggtttggct
     4321 tctgtgctgc agttcttcag catatcgacg ttgaacttcg gttcgttctc ggagctcgct
     4381 ttctctttcg cgctgtcgcc ttcgatcatc ataacctcgc tgggattttc cctgctcatg
     4441 ggcctgatcg gcggcttctt gcccgcgctc cgagcagcgc ggttgaatat cattcaagcg
     4501 ttaagagccg tgtagcgaca ttcggcccca tcgcttcgat tgagccgccc ggtcgtatgt
     4561 gagacgtaag ccgttcgcga tagtatcatc tgccagtgca tgtgcggcaa gagacgttga
     4621 gcggccctcg caaatcgatc tcacgagctt agttacaacg agctgaaaat tatcagccgg
     4681 ttctcgaaaa gagcattggg ccagtgttac cggtcagttc ggaatggggc ggcgaggata
     4741 tcgctcatgc ggggctatcc aaaggcgtaa ctttcaacga tcgaaaactt atcatctttc
     4801 gttgcgcttc atcatacagt cctagtcgca gcaaactgaa gcttttccag agagtgatgg
     4861 gtttaacttc ctgaaacgcc ggaatctcat cgtagcagcg ttgcaggttg tagttgggaa
     4921 tattgggtcg aacgtggtgg atatggtgca gacctatgtt acccgtaaac cactgaagaa
     4981 ctttcggcag cttgagatac gaactacctt ccagcgccac ccgcagagga ttccaagctt
     5041 cgtgccgcgc ccaataaaca ttttcaaact gatgttgaac ataaaacaac aacagcccga
     5101 gagttccgcc aaccaaaata atcggcagct gaatcagcag atacgtctta agaccgatag
     5161 tgaagccggc aacggcgacg attgccagta gcgccaagtt ggtgaagagc acgcttctgc
     5221 gctccctctt tccggcgccc ttggtaggaa atcgctggat gaacaagaac aagagcgctg
     5281 cacccggccc gaacaagacg aagggattcc gatagaatcg atacctgagt cgttttcgtg
     5341 cggaggctgc cagatactcc tcttttgtca tcgtccagat atcgccgata ccccggcggt
     5401 cgaggtctcc agccgtagca tggtgtgaat tgtggccata cttccaatcc tcaaacggtg
     5461 taaacgtgaa aatgcctgag acataaccga gaattgtgtt tgccgtccga gacgggaaga
     5521 aagaaccgtg acagcaatca tggaagagaa tgaagatacg gaccatcatt cctcccgcca
     5581 ctagcgccaa cgcgagagtg atccagtatg gatagcccct ctgaactgtt tgcaacatca
     5641 gggcccacaa gaggcaataa ggaataaacg tgtttagtag ttgcccaagc gctttgcgga
     5701 gatttgaaga tccgtaagca gcaatggtct tatgccacgt tggttcgaac cgtttcagtt
     5761 tgctagtagc tagattggca agcgggtcgc gcttcccggg ctgtagagct tgttcctgcg
     5821 gtaatgaatt ttccatgtaa caaatgatct cgcgtctccc aattacggcg gcgcctcagc
     5881 ctggcggtca aaccgaaccg ataattgaca agtaaacggc ccgggagtgc tagtaaatga
     5941 tcgatccctt ctcgatctgt ttttttcggt cgtcgtattc ttcacgggat attctcttgt
     6001 ttttatagtc ttcctccagc tgctgcatct ggcgataagc ctgatattcg taacctccgc
     6061 cagcggcgcc ggcacctacc aaggcccctg aaataaattc acatcccgat tgagcgagac
     6121 ctactataag cacggacagc aacattagtt ttttcattca aacctcctga atttttgaat
     6181 aggcacgaag caagctaaaa aaaccgccgg atggtcccta aactgccgcc gggaaaacaa
     6241 tcttgaaagt cgtaccgcgg ccagcttcgc tggtatggtc gatcgtgccg ttatgggcca
     6301 agacgatttg ttctacaatt ggcaggccca agccgctacc atgcaccttc gtggtctgga
     6361 ataatgcgaa aggatctata ccctctggca taccaatccc cgtatcagcg atctccaaaa
     6421 ctacagtggc gcccaaggag taacctctga tcgttagttc tcctcctcgc ggcatggcat
     6481 ccagggcatt gctgcagaga ttcaagatta cctgctttat ttttgccgtg tctaatttaa
     6541 cagccggcaa gccgtcctta aattcaagtt taaggttcac tccggcagtc tgatagacga
     6601 ccagctgtat cgctaatgca tcttcgacta cctgacgtaa atcactgcct gtaagattta
     6661 gattctgcgg aaccgaagcg gagcgaaact cctcaagcaa gatgatcaaa cgatcgactt
     6721 ctcgcctgaa actgcgcatc gttgttgtca atagcgggtc atggtttgtt ttgaggtcgc
     6781 gctcgatcag ttcgagagag gcggacatcc cctgaagcgg gctggcgact tcatgtgcaa
     6841 agagggcggt cgtagtattc agtaaagcgg taaaccgcct gacttgcgct ctttgctgcg
     6901 actgcttatg gtgcggctgc aagccgccgg ctattgccct gaggtgagta acgccgccgg
     6961 ttctatgttt ggttcccgaa gcagaggact tattcggaag atcgttttcc tcgccggttc
     7021 gctccattag ttgttgctcc cttatgtaag tatcatgtgc cagaggcact attcgaatcg
     7081 tcagatctct agactagcac tgagtatcgc gagaccgaaa tcggcactgt ctaatagtgg
     7141 acaatcgtga aaaatatttt gtgcgggtcc tcagtctatt aaggacctcc gcgctgacgg
     7201 tcggcagacg atatttgcgg ctcaccgcgt gattggtgcg atcactgtcc gcgagaagtc
     7261 agcggctaaa atcttctctg acctgagaac gttcgggaga ggatcagcgc ttgcctttaa
     7321 ccctgtttgc atcccaatga gccaaataaa aaatgactgc caaaacaagg atcatcacca
     7381 caaagggcgc tactgcttgc atcgagttct ccttatttcc ccgctgggct attgataatc
     7441 tgccccgggc gacgtgaaca acacctgagg gaggctgact tatgcggaat caagcattgc
     7501 tgacgccgcg ctcgttggtt atcgcgcctc gcatttagga ttcatatcga agttcgacaa
     7561 ttggtgccgt ctaatagtgg acaattttga aatgattaat gcggccgggc gtgcttaccc
     7621 cttgcatgac gtcgaaactt aaaggcgctt cagtttcggt cgctcctgcc atagcttcag
     7681 gattgaagct gtcagacgaa gtggcaattg catttctgct gcaaggcttg agcggggttg
     7741 cttgcttgga aaagaagagc aaagttcgct cgttgtgttt ggcaggagac cggcatcaaa
     7801 gaatttcgcc gctcgcaaag caaagtattt acttgaagca ctgcaagaga cgagattcat
     7861 cggcagaaga tcacggtaat ctcacagcag aggctcatga gtcccggtct gaacggcatg
     7921 ggtgagtctc tgggtttgcg gcaacctgat aaggcgcgta taagcaatgt acccctgatc
     7981 tcgcgatcca tctcgtgatc aataagcgga gtaaaagtat acaagatagc tgtttttttg
     8041 cttttgaaag ttcacaacga gccttagata taggatattt gaccccgatt tatgacaatg
     8101 gtggctagaa aaagcgaaaa aatctttgca caaaaagccg ccaggcccgc cagtcgaaaa
     8161 ttagccaagg ggcgacgaac cagcgactcc gctgcagtat ctgaagatcg gaaggacggc
     8221 tttcacatag tcggaatcgg cgcttctgcg ggcggtttga aagcgcttga aagttttttc
     8281 tcagccatgc cggtcaattg cgggatgagc ttcatcgtaa tttctcacct ggatccaaag
     8341 catagcagca tgctgcccga tttgctgaag agatacacca agatcatggt ggacagagcc
     8401 caaaacggca tacgtgtcga gcgcgaccgc atttacgtca tacctcccaa cacagaaatg
     8461 acccttgtac gcgggacgct ggtattgaaa aaaccttcgg agccgcatgg acagagattt
     8521 cccatagaca ctttttttcg ctctttagcg cgggaccgga agcagcgggc tatctgcatc
     8581 atcctctcgg gaaccggtac cgacgggacg tcgggactcc gggatgtcaa agccgaaggc
     8641 ggcatggcga tggcccaggc gacagcgtcg gccgaatacg atgggatgcc ccggagcgcc
     8701 attgcaaccg gtctcgtcga ttatagcttg ccggcggaaa agatgccggc ccagttgatc
     8761 aaatatgtga gcgaccgaca tcttaaagga gatgccggta cgcacgtgga acaacctgca
     8821 gaatcgatgg aggagttatt ccatctgctt gagtcccaaa ccggccatga tttttcatcg
     8881 tataaaaaga gcaccatcag caggcggttt aacagaagga tgggcgtgca gcgcatcaag
     8941 agcctgtctt cctaccttgg ttttattcga cgaaatccgt cggaaattga tgctcttttt
     9001 aaagaattgc tcatcggtgt cacccacttt ttccgcgatc ctgaggcatt taagagcctg
     9061 agcaaagtac tcagtgcgac tttacggaaa caccccaagg acagcacatt tcggatctgg
     9121 tctcccggct gctccaccgg ggaagagccc tactcgctcg cgatcctgct tgatgagtgc
     9181 aggagcaaac ttaagaaacg ctttcgcgta cagattttcg cgacggatgt cgacagcgag
     9241 gcaatcagct tggctcgcgc gggcgtgtat tcagggagcg ttgtctccgg actcacggcc
     9301 gagcggttga agcggttctt cctcaaagac aacggcgtat accgcgtcaa gaaagaaatc
     9361 cgcgacatgg tggtttttgc cgaacaagat ctcatcaggg acccggcgtt tagcaaactg
     9421 gatttgattt cgtgcagaaa cctcttgatc tacctggaaa cggaattaca aaagcagttg
     9481 ttgcgtcttt tccactatgc tctgaagccc ggaggtctgc tctttctagg cacctcagag
     9541 agtatcggca tcttcagtca tctcttttct ccagtcgata agaaatccaa aatttttaga
     9601 cggaggaatg gcggagcttt cgccaatgaa gccgttgggg agttccttgt caggcctcta
     9661 acgcatgccc gacccgaccg cgcgcctggg cggcccggaa agaagaagga cgacaacggt
     9721 ccggtcatca cgaatctgac tgaaaagaag ctcctggaag aatatacttc cccatctgtg
     9781 ctcatcaatc agaacggcga catcgtgtat gccttcggcc gaactggaaa gtacctggag
     9841 ctgcctcagg ggcacgccgc gttgaatatt cacgatatgg cccatccagg tattaaagat
     9901 gcgttggccg ccgccattca cgaagccgct atccgaaaca aacgggttgt agtggaaggt
     9961 ttgatcttga gccccgaggc ggaacttaac agggtcaaaa tcagcatcga gccgttacag
    10021 gtcaagccgg agggaagcgt cgggatgatg gtggttactt ttgaggatat tggcgttgag
    10081 atgaaagacg gcacgccaaa gcgcagggga gcgggctatg gcactcaact ggagcgcgag
    10141 ttgaagcttg ctcgacagcg tctcaaaagg agcatcgaag atttggaggt gtccaacgag
    10201 gagcttaaat cggccaatga agagtctcag tccactaatg aggaattgca aagcacgaac
    10261 gaagaattag aaacctcgaa agaggaactg caatcgatta acgaggaact ggtgacagtc
    10321 aatgcggagc ttcagggtaa gatcgaagag ttaacggtgg tgaacgacga cgtgagaaat
    10381 ctgctcgata acacaaatat tgccacgatt ttcctcgata agcagctgcg catcaagcga
    10441 ttcactcccg cagcgaccaa aatcgtgaat ttgatcccaa ctgatattgg aagacccctg
    10501 aatcatcttg ttttcaacct ggaattgccc gtccatgcgg aagcgaagaa agtccttgag
    10561 acgctcatcc ccaatgagat ggaggtgaga aacaatgagg gtcgatccta cctcagtcgc
    10621 atcatgcctt accgtaccgt ggacgatttg gttgacggtg ttgtgatcac attcacggat
    10681 atcactgaaa gtaagagggc tttggttcag caggagatgc ttgattatct taagagcatc
    10741 gtcgcgacga tccgcgattc actggtggtt ctggatggtg atttgaaggt cattacggct
    10801 agccggtctt tttaccaaac cttccaagtg caacccgagt ccacggaagg ggtgttgctc
    10861 tacgagttgg gcgacgggca gtggaatatc cccagcctga gagaattgct ggagaaaatc
    10921 ctaaccacta acgcttcttt tgaagatttc cccgttgagc atgattttcc tggcatcgga
    10981 cacaagaaga tgctcttgaa cgcccgtaaa gtttaccggg aaaatatcga tacgggaaga
    11041 attctgctcg ccatcgagga cactacggga gcggtgagcg caagctgagg cctcttactc
    11101 tgcgagaaag gcggactacg tgatgcgcga aaaagataaa gaagaagtca acctgcgtaa
    11161 gagggcggag gacttgctga aaaagaccgg caagagcacg ccgcggatcg tccatcaacg
    11221 tgctgccgag gtgatccacg aattggatgt gcaccgtgtc gaactggaga tgcaaaacga
    11281 agagctacgc agaagtcagg tcagcgctga agagtcgcag cgcaagtatg ccgagttgta
    11341 cgaatttgcc cccattggct tttgcagctt tgatcgaaag ggacagattc agcaggtcaa
    11401 cctcgcctgt gctcagatgc tgcgcataga gaagcgttat ctgcataaga gatggttccg
    11461 gagttttgtc agtcccgggt tccgggatga attcgaccgg ttctttcaca agctttttca
    11521 acccggacat cgagaatcct gcgagtcgca gctcacgcgc cacgacggcg ctctaattga
    11581 tgtcgaattg gtcgggggtg cggtagctga tcctgctctg ggcgttgccc atgaaagcca
    11641 gatagccatc ctcgacattt cacaaagaaa gaaggccgaa agctggtccc gcaggctggt
    11701 cgaaacaacc caggacgccg ttttatccat cgacagaaaa ggcagaatcg tgctgttcaa
    11761 tgcggccgca gagcaaacct tcggctatag caggaacgaa atagcggggc aaaaggtaaa
    11821 cgtactcatg tcggaaccct atgtgtcgca gcatgacgaa tatattgctc gttacgagag
    11881 gaccggcgaa gctcacgcca ttggccgaat tcgcactgtg acggcgaaac gcaagaacgg
    11941 cgatctgttt cccattgagc tgtctgtcac cgaaattcca accgacgagg acgttcacta
    12001 tgcggcgttt attcgcgaca tatccgacaa gaccaggctg caggagcaat tgctcgaaag
    12061 cgagcgactc gcggcgatcg gcgcgacggc agccaaaatc gcccatgaga ttgccaatcc
    12121 gcttaacaat atctatatga ccactcaagt actggagcgc gagctggcga aggaggagat
    12181 tccggtgcat ggacagctcg catccgatct taagactatc accagcgaga ctgcccgtct
    12241 gaacgcattg ctgcagcaat tccgcatgat ctcacggaag gagacctatg actttcatcc
    12301 ggtgagtatc gctgcgctga tcaaagaggt agcggacact tgccgcgcaa tctgggcgga
    12361 tcagaaaatt catttcgacc acgtctttga gccgaacctc ccatcgatct ctgccgaccg
    12421 tgacaaatta aaacaggtcc tccttaatct ctacaagaac ggcgcggagg ccatgccggg
    12481 cggcggcaag atcaccgtca aggcgtcgtc gacggaaact ggcgttcttg ttgaaattac
    12541 tgacaccggc acggggattc ctcccgatat ggatatcttc gaacccttcg ccacgaccaa
    12601 gctcggcgga acggggatcg gcatggtggt ggttcgtcaa gtagtcacgg cgcacggcgg
    12661 atcgattacg taccacagtg aaccgggcaa agggacgtca ttcttcattg cgttaccggc
    12721 tttaaaaagt tagatcagat gttgcgcggc gccgccgcaa tcaggcgatt tgcgcgagat
    12781 agtcggtgag cttactttct ttgatgatga gaaaacggtc tttgttggtc tcgatcaatc
    12841 ccagattgtg aaatctttgc ataaataagc tgatccgggg ccgggtggtt ccgaccatct
    12901 ccgacaattc ttcgtgcgta atcctaagct cgatgcgaat actgcgcggg tctttcttgc
    12961 ccattgttcg ggccagctgc aacaatgtct ggcccagccg ctgctcgcta tcgacagtca
    13021 ccagattggc gatgatctgc tgctggtcgg caatgcgcac ggcaaggtaa cgtacgaacc
    13081 cctgccagag cgagtcccgc cccaaacgcg cgaagaactg gatggaaggt atttgcttta
    13141 agctcgttgc cttcatcgca gtggctgtct ccaagcgttc gcctaagccg gagaggcaca
    13201 gctcgccgaa gatgtcgccc gcagtgtaga tgcccagtat gcattccttg ccttcgctgg
    13261 agagcatcaa cagctttact tggccggtct cgataaagta gatcatgccg tcgccatcgc
    13321 cactgttgta aacattttca tgtcgcggca ctttgacgga gcgggaattc tgcgtttcgt
    13381 tctgcaacga atcccgtaac tgttgtttga acttgtctat ctcaggattg tcttgaatca
    13441 taaaggctcc ttccaagagc tcgataattg cgaggggcta attaatcccc gcacgattat
    13501 gagtcgttgt tgcgagcgat ggagcgtctt tttgctagat atcgtttacc tcgaatatct
    13561 tgttccaggt acttgagttt atttcgtata tcgtgcgcgt caaggccgag ccaggcgcag
    13621 atgctcacaa aggaatacgg cgaatgttga tcaccactga agaaccatct cgccgcctcc
    13681 tgcgccctac accgctgctg atgaggcgag ccggcgacct tttcctgaaa acagagtaac
    13741 gcatcctcga gcacggcata catcaaagct gtctccgggc acacgctagc gaggctgatt
    13801 cgagtgttga agaactgctc cggaagcgcc ttgatattgg atcggcggtc atgcaagccg
    13861 tgccgttgtc gagcctcgga gcggcgatgt tttgggctgg ctggggcacg tttggaatac
    13921 atatcattct ccgatgttta aattccaata ggggtcgatc ctggcgtact ccgcatgcgg
    13981 attcatttag ccgacactgc cttcaaggct gacgcccaaa agtttctgcg cctcgacggc
    14041 aaattccatc ggcagctcgc gaaacacctg tttacagaaa ccattgacga tgagggtgac
    14101 agcgtcttca ggcgagatgc cgcgctgccg gcaatagaaa agctgatcct caccgatcct
    14161 cgatgtcgag gcctcatgct ccacctgtga tgagttgttc atcacctcta ggtaggggaa
    14221 ggtatgcgcg ccgcatttat cgcccaggag caacgaatca cattgcgaat aattgcgcgc
    14281 accggcagcg cccttcatga ccttcactag cccccgatag gtgttctgcc cgtggccggc
    14341 cgagattccc ttcgaaataa tcgtgctctt ggtgttcttg ccaatatgga tcatcttggt
    14401 gccggtatcg gcctgctgat agttgttggt cagcgccacc gagtaaaatt cacccaccga
    14461 gttgtcgccc tgcagtatgc agctgggata cttccaggtg attgccgatc cggtctccac
    14521 ctgggtccaa gagatcttgg agttgttacc cagacacttg ccgcgcttgg tgacaaaatt
    14581 gtagattccc cccttgccgt ccttatcgcc gggataccag ttctgcactg tcgagtactt
    14641 gatctgggca ttgtccaggg caaccagttc cacgacggcg gcatgcagtt gattttcatc
    14701 gcgcatcggc gccgtgcagc cctcaaggta gctgacggag ctgccctcat cggcaatgat
    14761 caaggtgcgc tcaaattgcc ctgtttcagc ggcattgatg cgaaaataag tcgaaagctc
    14821 catcggacag cgcacgcctc tggggatgta acagaacgat ccatcactga agacagcggc
    14881 gttcaaggcg gcgaagaagt tatcactgta cggcaccacg gagccaagat attttctaac
    14941 gatcactgga tgcttctgca ccgcttcgga aaacgaacaa aagataactc ccaacgacgc
    15001 cagcttttcc ttgaatgtcg tcgctaccga gacgctgtcg aagactgcgt cgacggcgac
    15061 gccggtcagc cacttttgct ccagcaacgg aatacccagc ttgtcatagg tttcgagcag
    15121 cttgggatcc acctcttcga gactctttag ccccacgttg gtcttgggcg ccgaatagta
    15181 gatgaggtcc tgatagtcga tcgccggata gtggacattg gcccattttg gctctgcctc
    15241 ggactttgcc agcttgaccc agtggcggta ggccttgaga cgccagtcca gcataaaact
    15301 cggttcgttc tttttggccg aaatcaagcg gacagttgcc tcgttgaggc cgcgcggtac
    15361 ggtgtcagtt tcgatttccg taacaaaccc atacttatat tcctggttga ccaggtcctc
    15421 agttgtttgc gtggaagtgt tcatggctgc agttttccta ttcacgagac tcgttagcga
    15481 tctccggtaa attttaagac tacaccggca tcgatgtgat tgctgtctaa tagtgaacgg
    15541 ttcagagtct caagactcga gaagcaggtc ggcggtcacg ccgaggcgtt taaggtgagt
    15601 tcgccaatag cagccagcgc cagccgattg agtttctggg ctggtcagtt ctgctgcttt
    15661 ctgagaaatt cgtcgttaga tcaggtagat acgctattta atctgttcaa tctttgcaaa
    15721 acagcgatcg aaaaacgcgc ccaagggacc gcgcgccgcc tttctcatga tgccatgcag
    15781 ctcggctggt tttagccaaa tgccttgaca cctgtcacag cgttgtagga caaagcccat
    15841 gaagccatac ttttcgagtt tgcctgcgca catgggacag ttaccgacct gtctttcccg
    15901 tctggctgct tcagcgtttt gaaagtcgac cttcatctcg tcaatacgtt catgctcttt
    15961 taacgcaaaa tatttgtctt cgttggctct ctcatcaaac tctatgtttt cacctaagcg
    16021 attttgattc ttcatggctc tacctcattt ttaataactc ttcgcctgac gcaaaattgc
    16081 attgtgcctc aatctaattc cgctctactc gtcggcccct ttgcttccca gtgattcagg
    16141 cgctaaaaga ggtgccacat ccacaggtgc ttttcacgtt gggattgata aatatgaagc
    16201 ccgattgcat taggtcgtct ttataatcga gctccgttcc gttcagatga atggcgctag
    16261 ttggatcaag caagatgcgc gcgccgtttc tctcgatgac ccggtcatcc gctgcaatcg
    16321 cgtcgatggc catcttgtac tcaaagcctg aacagccgcc ggcgacaact ttgagacgca
    16381 agcctttttc gtcggtctca ctaccaagca gtcgccgaat cttttcggcg gcgcgatcgc
    16441 tgagggtaat ttccccgacc atattgaggt tctcctttgc ttagattgcc agctccggct
    16501 cctgacgagt tttcttaacg agccattttt cgcgcatgat cttgtcctgg atcctgagca
    16561 gtccttctgt aagcgcttcg ggtctcggcg ggcagccagg aacgtagacg tcgacgggga
    16621 cgatcttgtc cacgccttta caaacggaat aggcgagctg aaaaagccca ccgcaactgg
    16681 cgcagcttcc catcgcgata acatatttgg gatccggcat ttgatcgtag aggagcttgg
    16741 tacgcttggc cattttataa gtgagggttc ctgccacgat catcaaatca gactggcgag
    16801 gcgtggcgcg aggaaccgcg ccgaatcgat cgacatcggc gcgcggccct ccagtctgca
    16861 ttaactcgat ggcgcaacag gccaagccga aaagcatgta ccactgcgac gaggctcggc
    16921 cccaattaag aagatcgtct accttcgtgg tcaacacgtt ttcaggcaac tggtttagta
    16981 gtgacgtaaa agcctccttc gacacccagc ggatggtgat gcctgcaggt ccgatcgtta
    17041 tgtaagcgag tctctgattc ctgaaaggag aggacgatct ccgctattga tcgaccttac
    17101 cttgcgtcaa tatgccgccc tggagatggg aacttacgcg gccatttgac tgatgaccgc
    17161 cgggcatgga cttctcggga aagtctcttg aatcactgta agccaaccgg ccctactcgt
    17221 aatcgcttgc ggattgtaga attaataaca caggagcata ccctgactgt ggacctaata
    17281 agtccatctt gcaatagacc ccttgttagg ctcagagtat tgcacactga tcaacaacgc
    17341 gctgccactg caattggaga tgtgctcgac tcggagtggt agagttgagc gccacctagc
    17401 acgcgttaaa aatagtgtgc cccctgacag agcaaagatt atgaaaaaat acaaaaaaat
    17461 tccccaggta ggatcggtta tgaccccgtt tccctattcc gtcgataccg atgatacgat
    17521 tgcagaaatt gagcgtctga tggatgagca ccagattcgt catgttccgg tccaacagga
    17581 tgggcgggtg gtcggaatca tctcggaacg ggatgtctat cgtttgctcg aacgcttttt
    17641 gccacaggca caaaaggagc agattcgagc tcgcgatatc atgatcgccg atccgtacgt
    17701 tgtttcattc gaggcgccgc tcaacgaggt cgctcttgaa atggcaaaac gccacatcgg
    17761 ctccgtcatc gttcttcatc acgagaaact tgcgggtatc gtatcggcaa ccgatatatg
    17821 tcgtattctg gcggaaatat tcgagaccga atttgccgcc gcaaccggcg atgatgtcgc
    17881 atagcgcaat gtgcctttat tgttaacgat ttccatctga gccggtatct gctatggtgc
    17941 caatatggga aatcaaacat atcagaagcg ccagaaagag ttggcgcgct cacaaaagaa
    18001 aaaagacaag gccgcgcgcc gaatgcagcg gaaaacggaa aaggtaaaag gcgggccgcc
    18061 gttggaggac gaccccatga ccggcggccg aatgacctcc gagtcaagcc tgcaatcttc
    18121 tcctgccgtt tgagaaggac cacgctcctt gcaactagca agtcctaagg cagcccaatc
    18181 ggcgccgcct cagaacgtgt gttgcccgcc gaatcctagc gcgctttgaa cttagttgac
    18241 acgagttggg tttagacata ggttcagtta tcttcttcag taaatggcct tctgaggaaa
    18301 atgatatgtg cgaaggcctt tgatcagtct ctctccttgc gaagcaatct atggatgaga
    18361 tcccgacctg gagcgtggtt taagagttca gttccgccca tgttctgccc atttgcagtg
    18421 cggttctcag ggtcaaagac aataacattc tctcatagga ggagaacatg ggtagtaaat
    18481 tatatgtcgg tggattgcct tattccgtaa ccgaaggacg cttacaagaa gttttctcgg
    18541 cgcacggtac ggtagaatcc gcgaacgtca tctcggataa atttaccggg cagtcacgag
    18601 gttttggttt cgtggagatg ggctcgtctg gcgaggcgca aagagcaatt tctgcaatga
    18661 atggtacaca attggatggc cgcacgctga ctgtgaatga agccaagccg caggccaggc
    18721 gcgatggcgg tggggaccgc gaaggccgcg gcaaccgcga cggcggacgc aaccgctggt
    18781 aaatttttcc tggttaggta agcagtaggg aagcagcagg tcgggttgct tccctatttt
    18841 ttcgccaccg ctcacttccc cagcctccgt tccattaagt atctttcaat tggcgcgtgt
    18901 aggtaatacc atcgtacatg cgtgagggct agctcctact agatagccgc gtaccgagct
    18961 tagcttacat caaggggtat ctttatcgtc cgggtctgtt atagtcgtta gcgacgtaac
    19021 tactggcaaa ccgaagttca cataaagtcg ctcagtccaa tttcggctga gtttcaaaga
    19081 ggcggttccc tagggtatcc gcctctttgt ctttttagcg tcatactaaa ctgcaaacaa
    19141 agtcgccgct cagaaaaaga ggtcgtttat gcaaggtagt tccagcccat cggctatcga
    19201 ccgcatcgct ttcatcggta actatctccc gcgccagtgc ggcatcgcca cgttcaccac
    19261 cgacttgtgc gaggcaatcg ccgctgagca cgatgacacg agctgtatcg ccgtgccggt
    19321 taacgatatc gaaagcggtt atgcctatcc atctcgtgtt cggttcgagc tggtagagaa
    19381 agatatcgag tcgtaccgcc gcgccgccga tttcttgaac atcaacagcg tggacttggt
    19441 ttgcttgcaa tttgaatatg gaatctttgg cggacgcgcg ggaagccaca tcctggcgct
    19501 actgcgtgag ctgcggattc cgatcgtgac gactctgcac acgattctgc gcgaaccgga
    19561 tccgcatcaa cgccgggttt tggaggaagt cgcagctctg tcggatcggc tggtagtcat
    19621 gagccagcgc ggcgccgagt ttctcgaaac catttatcag atatcgccgt acaaaatcga
    19681 tcttatcccg cacggtattc ccgacgtgcc ttttgtcgat cccagtttcc acaaagatgt
    19741 tttcggtgtc gaagggaaaa ccgttttgct cagttttggc ttgctttcgg cgaacaaggg
    19801 tatcgaaaac gtcatcgcgg cgctccccgc tatattggcc aaacatccga acgttgttta
    19861 tttcattgtc ggtgcaactc atccgcacgt caaacggcag gacggcgaga cttaccgatt
    19921 gtcgctgcaa cgcttggcgc tggacagggg cgtagccggt cacgtcattt tctataaccg
    19981 cttcgttagt atggaggagc ttgtcgagtt catcggcgcc accgacattt acatcacgcc
    20041 ttatctcaac ccggcgcaga tcgtttcagg tactctcgct tataccgtcg gcgcgggcaa
    20101 ggcggtgatc tccacgccgt actggcatgc tgaggagatg ttggcggaac agcgcggcgc
    20161 gctggtaccg ttccgtgatc ccgtggcatt ggcggatcaa gtaatcgact tgttaagcaa
    20221 tgacgccaag cgtaacgcca tgcgcaagcg agcttatatg tacggcaggg aagcgatctg
    20281 gccgcaggtc gcacggcgtt acatggaaac ttttcagcgg gctcgagccg aacggcgcca
    20341 cttcgctcca tcggactttg ccgttaaacc gttggataaa cgtcccggcg aatttcctcc
    20401 tctcaagctt gaccacttgc agcgcatgac cgaccacacg ggtatgtttc aacacgcgat
    20461 ttttaccgtt cccaattatc tggagggtta caccatcgac gacaatgccc gcgctctttt
    20521 ggtcagcgct ctcttggagg agctgggaaa ctcggaagct tatgaacttt catcccgtta
    20581 tctcgcgttt gtttggtacg cgtttaattc cgaaactcgg cgcttccgca attttatgga
    20641 cttccagcgc aattggctcg aagaaagcgg ctccgacgat agccacggcc gcaccttgtg
    20701 ggctttgggc acgttgctcg gtcgatccag cactccggcc cttcagagca cggcgggccg
    20761 gttgttcgag caggctttgc cgttaatcag cgacaccacc agtccccgcg catgggcatt
    20821 tgcgttgatc ggcatacacg aatatctgcg tcgtttcgat ggcgatcgcc gtgccagtca
    20881 agtccgcgaa caactggctg ggcgactgct ggctttattt gaaaagaatc gcgccggaga
    20941 ctggcgctgg tatgagagct cgctgaccta ttgcaacgct gcgttgccgc acgctttact
    21001 gatgtccggc caacggatac ctaactataa aatgatcgaa tcaggcttgg agtcgcttca
    21061 gtggctggcc gagttgcagc gcgccgacac caacggcggt cactttgtgc cgatcggctc
    21121 caatggcttt taccaacgcg gcggcgagcg cgcccggttc gatcagcagc cggtggaagc
    21181 acaagcgatg gtgtctgcct gccttgaagc ttatcgagtc acgggagaca aaggctggca
    21241 caatgaagca cgacgcgcct tcgactggtt tctaggacgc aacgatttga acctgcccat
    21301 ctatgatccg gtaaccggcg gatgccggga cggcttgcat tccgaccgtg ccaatgaaaa
    21361 tcagggcgca gagtcttctc tggcctatct tcaggcggta ctggaaatgc gtttggcgga
    21421 aacaacatta ctatcggtgg aaactggagc tcgatgacca accaacatca ggaacttttt
    21481 cgccgtcaga aaaccaatcc tatattgagc gctaacaatt ggccctatcc aatcaacagc
    21541 gtttttaatc cgggcgcgac gttgctcccg gatggcacga ctctgctgtt gtgccgggtc
    21601 gaagatcgcc gcggtcattc tcatctgacc gtcgctcgtt cgaccaacgg cgtggacaat
    21661 tggcagatcg aatcacagcc aactttccgg gcggatccga aacagttccc cgaagagtta
    21721 tggggcatcg aagatccgcg cattacctat atccctgagt tgagcaaata tgcgattgtc
    21781 tataccgctt tcacacgcga tggcccggga gtttctctcg ccctgaccca agactttcgt
    21841 gagttcgaac gctatggtgt cattatgccg cctgaggaca aggatgcggc tttgcttccc
    21901 taccgtatcg gaggccagtg ggctttgatt caccgcccgg tcagtgctcc tcgtgcacac
    21961 atgtggttgt cttactcgcc ggacctgcgg cactggggca atcacaagtt gatgttggaa
    22021 gcacgtcgcg gagcgtggtg ggatgccaat aaaatcggtc tttcgccgcc gccgatagaa
    22081 acgcctcagg gttggctggt catttaccat ggcgtccgta acacagcgtc gggcgccatc
    22141 taccgattag gactggcttt gtttgatttg gacgccccgg aacattgctt gaagcgcggc
    22201 gacgaatgga ttttcgggcc acaagcgacc tacgagcagc gtggcgacgt cgacaacgtg
    22261 gtatttccct gcgggtacac gttagagtct gacggcgata ctcttcggtt atattacggc
    22321 gctgcggaca cgagcatcgg actggcgaca ggtagtttgc ggcggatgct ggagtggctt
    22381 gagaaccatc agtcaaa
//
LOCUS       DASUVS010000129         7584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_266803, whole genome shotgun sequence.
ACCESSION   DASUVS010000129 DASUVS010000000
VERSION     DASUVS010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7584
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266803"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(372..944)
                     /locus_tag="VF182_07290"
     CDS             complement(372..944)
                     /locus_tag="VF182_07290"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEX6436918.1"
                     /translation="MSSLTLQMPALPDPVKVVLQPATTALLVFDMIDRICKNQPNCTG
                     IMIPALGSLLARARKSGVFVLYSTREPESSTWMPEVAPAPGDPIVKSVGQDRFYSTDL
                     DKMLKAKGIKTLVLTGWKVSGSVVYTSVGATLRGYTVVVPTDASLAATDYEVAIGQFQ
                     ILNQNSANATNEPLKANASTLSRADMIVFQ"
     gene            1576..3033
                     /locus_tag="VF182_07295"
     CDS             1576..3033
                     /locus_tag="VF182_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX6436919.1"
                     /translation="MKGSAKITRQESDAVHSQPSILSSAVVVASLSAFLGSLDSALNI
                     SFPAITAAFSLEVSSIQWVIVGYVLPHASLLLGCGRLADIWGHGRVLTWGLLSSSLAF
                     LGCGLAPSFGWLVAARAAQGLGAALISGSAPALVTLAVPPAARGRALGIYQMSMAAGF
                     AAGPLLGGVLVDLFGWRAVFLFRLIPAMLLVWLTAAKLPALRESREPEGFDLAGALTL
                     ALSLASLLLAISQSRRAGWSSSGVIALGVGFAACFSGFIIMERRARAPVVDLRLFRHV
                     PFVIANLLAVLANCARFAVGLLVPYYVIEVLRYSAATGGTVMLAAAVMTPLAAPVAGR
                     LSDRFGTAALSALGLALEGLGLWMVSRLDSQSEYLWVAVALCIVGLGLGVFETPNMSF
                     VMGSVARQQQGVAGSIANMMRPLGILVGATGWSMLLDARREFHSQSPSMGAAAAFPGF
                     VPAFQDVFAVAAALCAVAWILSLFRVRKSWSLKHL"
     gene            3154..3351
                     /locus_tag="VF182_07300"
     CDS             3154..3351
                     /locus_tag="VF182_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436920.1"
                     /translation="MKANLLDAFKLVMQCKRELVEESLKARHYPYFISISPFALMALV
                     HSLHTVCIGKTSFLFFNGLPE"
     gene            3415..4653
                     /locus_tag="VF182_07305"
     CDS             3415..4653
                     /locus_tag="VF182_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HEX6436921.1"
                     /translation="MAQTVVQISGHIIDSLILPKVLDLIISLGAQFEILDIQIGHRRS
                     DRSHARIKVEASSPDLLDVVLAKIREHGALPEQVEEESLQLEAAPLDGVFPERFYATT
                     NLETWVRLEQQWIPVESIEMDCGIRIDVSARKAQCVPMHRVKKGDLVVVGQRGVKVVP
                     LERQQPGAPFEFMASPVSTEQPKGLLIREIAESMQRVRRDNGRILVVAGPGLVHTGAS
                     KYLAELIENGYVQVLFAGNGLAVHDMESDLYGTSLGVYVDQKIRAAEGHEHHLWTINR
                     IRKAGGIRQAVEQGLIRKGIFYSCVKNDVEFVLAGSIRDDGPLPDVITDTLQAQDIMR
                     EKARGVSFALMIATTLHAIATGNLLPASVKTVCVDINPAVVTKLTDRGTFQALGLVTD
                     VEPFLRELCSDLDLNVVCSS"
     gene            4653..5432
                     /locus_tag="VF182_07310"
     CDS             4653..5432
                     /locus_tag="VF182_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEX6436922.1"
                     /translation="MCPPSHYGIEYEINPWMKRSRQSDATLATQQWNELQRLLAEKLP
                     AEIHLIDAIPGLPDMVFTANAGLVWQNKFIPSNFRHEVRRGEAPHFEEWFRARGFEIV
                     QLPEKYYFEGEGDLLRCGELWFAGYHIRSDMMAHQKVAEIMDREILSLELTSGWFYHL
                     DTCFCPLSDKTALFYPPAFDDYAKTVLEEHIPNLIPVPANEAERFACNAVVGGNNVVM
                     NTGCPLARGKLEDRGFDVYETPLDEFLKAGGSAKCLTLIMG"
     gene            5623..6252
                     /locus_tag="VF182_07315"
     CDS             5623..6252
                     /locus_tag="VF182_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436923.1"
                     /translation="MKTSFSVALILLLSGCAGFQAAREVNSGRQAYLIGNNEAAYAHF
                     QKAAEVDPNYVYGTALRQGVWSYVGRVNYDMGRLPQAREALERALAANREEDLSRVYF
                     GLALAREGDRQRGLKEIEGGLRGVHQWLEYVDQNHGASFGRFWDPAREIRSAIQTDLA
                     MISGRDIDWQKLIASGEWVGKRMELESDRARRDETMERNRESDSRGDTR"
     gene            complement(6525..7451)
                     /locus_tag="VF182_07320"
     CDS             complement(6525..7451)
                     /locus_tag="VF182_07320"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX6436924.1"
                     /translation="MIFAMLISENTMDVFTEHLRTFLKVCELKSFTKAAQTLRKTQST
                     ISTQVAVLEQDTGLQLLDRSQRPIHLTEAGEIFSSLARQIVNSTDQLGRSIRELAGGV
                     AGEVKIATTTSIGAFILPKIVGRLLQEMPRVRLDITIKSLSSVCESVKQAETDFGIIL
                     SQREPPALVAKSLKPERLCFVVSPGHSLAGSMVRLEELRSHSFVVGARTGGYTEMIQR
                     LLERNGLFGFEVALRVGSFEAIKEAVRSGLGIGVLPEFMVRREIRSGVLAEISTKKNN
                     FSANITLIERPGYTPTPTVESVRGFIHKMMTS"
BASE COUNT         1615 a         2027 c         2199 g         1743 t
ORIGIN      
        1 actacgtgaa caaggaatca gaggagatgg atgttttcaa gcccacaata aataagacgt
       61 cgaataggca acaggcagca gtaagaagag aactcgttca acggttcagt cgttcaaacg
      121 ttcaacgtcg aatttatccg aacacggcct tcattttgcc ggcaaaagtg aggctctaag
      181 gcatcaagac acattcattt ggaatgaaaa catcgatcat gcaacgacat cgcttgctcc
      241 gacgaaatac aacgaagcaa gttcaatcgt tccagccgtt caaccgcttc gctcggtcca
      301 aaacgtcttg caagcaatac caaagcgttg tgatgtgatc gaagcagaca acgtatcgcg
      361 aagctgcctt tctactgaaa gacgatcata tccgcacggc tcagggttga ggcgtttgct
      421 ttcagcggtt cgttggtggc gttcgcgcta ttctgattga gtatttggaa ttggccgatc
      481 gccacttcgt agtccgttgc ggctaagctg gcatccgtgg gtaccaccac agtgtacccc
      541 cgcagcgttg ctccgaccga tgtatagacc accgagccgc tgaccttcca gccggtgaga
      601 accagcgtct taatcccctt ggccttcagc attttgtcga ggtccgtgct gtagaatcgg
      661 tcctgaccaa ccgacttgac gatcgggtcg ccgggagcgg gagcgacctc cggcatccac
      721 gtcgagctct ccggctcccg agtggagtac aggacgaaca cgccggattt ccgtgcacgc
      781 gccaacagcg agccgagcgc cgggatcatg atcccggtgc aattcggctg gtttttgcag
      841 atacgatcga tcatgtcgaa caccaacaac gccgtcgtcg cgggttgaag gacgactttg
      901 accgggtcgg gaagggcagg catttgcagt gttagcgatg acatgggttt cctccgtagc
      961 gcgtgagcgt tagcaggctg ttgaaaaggc ctaccgagac cctacaaaag ctgaattatg
     1021 aaagctgaaa ggctgaagac cgactgagaa ctgaagattt ccgctttcat ccttgtcttt
     1081 tccgcctcgc atctggacct ttttcgaacg gcctgaaatc aagtttttgc gtcatgttag
     1141 atttgcgagc acaggtcttg tgaaagtaca gtcgcagcgt ctatgtacgc tgggctccaa
     1201 gtccgaaact taatcactct caatctttga gagagaaata tcgccgacgg gagaacgtgt
     1261 caaacgtcaa ataggcaaca ggcaacagtc aatagtaaga aaagaatttg ttcaacggtt
     1321 caacgggttc aatggttcag atgttcgtga tcgttcaagc cgttcaagcc cctggatcga
     1381 gtccagggtc cgttgaagtc gttaagagcg gaagagcggt tcccgttgtt cccgtctttt
     1441 cagtggcttg aaacgccgac gggtagtgcg agagttggag aacggactgg tttgtcgaga
     1501 acgtcagaaa acagattgcc gcccggcgtt tcatgctaca gtggcgctct ccaattaaga
     1561 tcgtgaaccg atcatgtgaa ggggagcgcg aaaattaccc gccaagaatc tgacgcagtc
     1621 cattcccaac cttcaatcct ttccagtgcc gtcgtcgttg ccagtctgag cgcctttctc
     1681 ggttctctcg acagcgcact gaacatctcc tttccggcga tcacggcggc gttttccttg
     1741 gaggtcagct cgatccaatg ggtgatcgtc ggctacgtgt tgccgcacgc gagcctgctg
     1801 ctcggctgcg gccggctcgc cgatatttgg ggccacgggc gggtgctgac atggggattg
     1861 ctgtcgagct cgctcgcctt tctcggctgc ggattggcgc cgagcttcgg ctggctggtc
     1921 gcggcgcgcg cggcacaagg tctgggcgcg gcgttgatct cgggatcggc tccggcgctg
     1981 gtcaccctgg cggtgcctcc ggcggcgcgc ggtcgcgctc tggggattta ccagatgagc
     2041 atggcagcgg ggtttgccgc gggcccgttg ttgggcggcg tgctggtgga tctattcggc
     2101 tggcgcgcgg tttttctgtt tcgtttgata ccggcgatgt tgctggtctg gctcactgcg
     2161 gccaagctgc cggcgcttcg agagagcaga gagccggaag gtttcgatct ggcgggagct
     2221 ttgactctcg ccctcagttt ggcgagcctg cttttagcga tcagtcaaag ccgccgagcg
     2281 ggttggtcgt cgtcgggcgt gatcgcgctg ggagtcggtt tcgcagcttg cttttccgga
     2341 ttcatcatca tggagaggcg ggcgagggcg ccggtcgtcg acttgcgcct ctttcgccat
     2401 gtaccgttcg tcatcgccaa cctcctagcc gtgctcgcca actgcgccag attcgccgtc
     2461 gggttgctcg tcccgtatta tgtaatagag gtgctgcgct attcggcggc gaccggcggc
     2521 actgtgatgc tggcggcggc ggtgatgacg ccccttgccg cgccggtggc cggcagattg
     2581 tccgaccgct tcggcacggc cgccttgagc gctctcggac tggctctgga agggctgggc
     2641 ctttggatgg tcagccgatt ggacagccag tccgaatacc tttgggtcgc cgtcgccctt
     2701 tgcatcgtgg ggctcggtct gggcgtgttc gaaactccca atatgagctt cgtgatgggt
     2761 tcggtcgcgc gccagcagca gggagtggcc ggcagtatcg ccaatatgat gcgtccccta
     2821 ggcatcctcg tcggcgccac aggatggagc atgctgttgg acgcgcggcg ggagttccat
     2881 tcgcagtccc cgtccatggg cgcggccgct gctttcccag ggtttgttcc ggcctttcag
     2941 gatgttttcg ccgtcgccgc cgcgctgtgc gcagtcgctt ggattctatc cttgttccgc
     3001 gtgcgaaaat cttggagtct aaagcacctg tgaagggagg agaaaaaggg ggaggctact
     3061 gattcgttga tagccacggt ggtgcgtgct agaagcgcgc gatgccgcgc atagataccc
     3121 gccccaattt accagggcga gactctcgct gaagtgaagg cgaatctact ggatgccttc
     3181 aagctggtga tgcaatgcaa acgcgagttg gtggaagaat ccctcaaagc tcgacactat
     3241 ccatatttta tttcaatctc gccgttcgct ttgatggcct tggtccattc gctccatacc
     3301 gtctgcatag gcaagacgtc ctttctcttt ttcaacggcc tgccggaata attgcgtcac
     3361 gttgtgcaaa gttgcagtta acattacaat cgaggcctac gggaggagtt cgcgatggcg
     3421 cagactgtcg tacagatctc cggtcatatt atcgactccc tcatactgcc caaagttctc
     3481 gacctgatca ttagccttgg cgcccaattc gagatcctgg atatccagat cggtcaccgc
     3541 cgcagcgatc gtagtcatgc gcggatcaaa gtcgaagctt caagtccgga tctgcttgac
     3601 gttgtccttg cgaaaattag agaacatggc gcgctgcctg aacaggtgga agaggagtcg
     3661 ttgcaactcg aagcggcgcc gctcgacggt gtttttcccg agcgctttta tgcgacaacc
     3721 aacctggaaa cctgggtgcg actggaacag caatggatcc ccgtcgaatc gatcgagatg
     3781 gattgcggga ttcgtatcga tgtaagcgcg cgcaaagcgc agtgcgtgcc gatgcaccgc
     3841 gtaaaaaaag gcgatctcgt cgtggtgggc caacgcggcg tcaaagtcgt gcctctggag
     3901 cgccagcagc ctggagcacc tttcgagttc atggccagcc cggtgtcgac agagcaaccg
     3961 aaaggactgc tgattcggga gatagcggaa tcgatgcagc gcgtgcgccg ggacaatggg
     4021 cgcatactcg tggtggcagg accgggactc gtccacaccg gcgcgtcaaa gtatctcgcc
     4081 gagctgattg aaaacggcta tgtgcaggtg ctttttgccg gcaacggact cgcggtccac
     4141 gacatggaat ccgatctcta cggcacatcg ctgggtgttt acgtcgacca gaaaattcgc
     4201 gccgccgaag gccatgaaca tcatctctgg accatcaacc gtatccgcaa agccggcggc
     4261 atacggcaag ccgtcgagca gggactgatt cgcaagggga tcttttattc ttgcgtgaaa
     4321 aacgacgttg agttcgtgct cgccggatcg atccgcgatg acggcccgct ccccgatgtc
     4381 attaccgata ccctgcaggc gcaagacatc atgcgcgaga aggcgcgcgg cgtgagtttc
     4441 gcattgatga tagcgaccac cttgcacgcc attgccacgg gcaatctctt gcccgcgagc
     4501 gtcaagacgg tgtgcgtgga catcaaccca gcggtcgtga ccaagctcac cgaccgcggc
     4561 actttccaag ctctgggttt ggttacggac gtggaaccgt ttttgcgcga gctgtgcagc
     4621 gacctggact taaatgtcgt atgcagctct tgatgtgccc gccgtctcat tacggcatcg
     4681 agtacgagat caatccgtgg atgaagcgaa gccgccaaag cgatgcgacg ctcgccacac
     4741 agcagtggaa cgaattacag cgcctgctgg ccgagaaatt gccggcggag atccacttga
     4801 tcgatgccat accagggctt cccgacatgg tctttaccgc caacgccggc ctggtctggc
     4861 aaaataaatt tatcccgagc aacttccgcc acgaagtccg caggggtgaa gcgccgcatt
     4921 ttgaggaatg gtttcgcgct cgcggtttcg agatcgttca actgccggag aaatattact
     4981 tcgagggcga aggcgacctg ctgcgctgcg gcgagttgtg gtttgcgggc taccacattc
     5041 gctccgacat gatggcgcat cagaaagtgg ctgagattat ggatcgggaa atactctcgc
     5101 tggagctgac cagcggctgg ttctaccacc tcgacacttg cttctgcccg ctctcggata
     5161 agacggcgct attctatcct cccgcctttg atgattacgc caaaacggtg cttgaagagc
     5221 atatcccgaa tctcattccc gtgccggcga acgaggcgga gcgctttgct tgcaacgccg
     5281 tggtcggcgg caacaatgtc gtcatgaaca cgggttgccc gttagctcgc ggaaaactcg
     5341 aagatcgcgg cttcgatgtt tatgaaacac cgctggatga gtttctcaaa gcgggtggca
     5401 gcgcgaaatg tctgacgttg atcatgggat aaaatttctg ccgccgaaca agccattcac
     5461 agggcaaatc gtttgcggtc aggcagttga atcgtacgca acgctattgc tacggcgatc
     5521 ttttgcaaag ccggggcatg aagcggctga tggagtgtca caaccgatgc cggattgttt
     5581 ttttacgtgt gtagaattat ctttgatgat caaggaggac ttatgaaaac cagcttttca
     5641 gtcgccctaa ttctgctcct ttcgggttgc gccggctttc aagccgctcg agaggtcaac
     5701 tcaggaagac aggcttacct tatcggcaat aacgaagccg cctacgcgca tttccaaaag
     5761 gctgcggaag tggacccaaa ttatgtttac ggcactgcgc tacgccaggg agtctggagt
     5821 tacgttggcc gggtgaatta cgatatggga cggctccctc aagcgcgcga ggcacttgag
     5881 cgcgcgctgg ccgctaatcg ggaggaagat ctttccagag tttatttcgg ccttgcgtta
     5941 gctcgagagg gcgaccgcca gagaggcctt aaggagatcg agggtggctt gcgtggagtg
     6001 catcaatggt tggagtatgt ggatcagaac cacggcgcct cgtttggccg gttctgggat
     6061 ccggcgcgcg aaatccggtc ggccattcag acggacttgg cgatgatctc cggcagagac
     6121 atcgattggc aaaaactcat cgcctccggt gaatgggttg gcaaacgaat ggaacttgaa
     6181 agcgatcgtg ccagacgcga cgaaacgatg gagcgaaaca gagaaagcga cagccgaggg
     6241 gatactcgtt aatagaatgc tgtcgtaaaa tctgccgatt gtttgcagca ggtcgttctt
     6301 gtgaaatttc gagaagagcc acgagacatt tggatctgag ccgtattctc ctcgtatcgc
     6361 gcggcgcctc gggtgtctca gcagctgcag ataccagccg atagcttcgt ccggattcct
     6421 cccgattcgg tgagaggcat gagttcttga tctatccgtg tgcctagata tagaggcagg
     6481 taatggcgcg caagccgaga gtcgagttca ggatcctcgc acaactaaga tgtcatcatc
     6541 ttatgaataa accctctcac cgattctact gttggtgtcg gcgtatatcc gggccgttcg
     6601 ataagtgtaa tatttgctga aaaattattt ttcttcgtag aaatctctgc aagtactccg
     6661 ctgcggattt cgcgtcgaac catgaattca gggagcacac caatccccaa gccagaccgc
     6721 acagcttcct tgatcgcttc aaagctgccg acgcgcagcg ccacttcaaa gccaaatagc
     6781 ccgttgcgct cgagcaatct ttgtatcatc tctgtatatc cgccggttct ggcgcctact
     6841 acaaacgagt gtgagcgcaa ctcttccaat ctgaccatgc ttcctgcgag agagtgaccg
     6901 ggcgaaacaa cgaagcatag gcgctccggc ttgaggctct ttgcaacaag tgccgggggt
     6961 tccctttggg aaaggattat gccgaagtcg gtttccgcct gtttaactga ttcacacact
     7021 gatgacaaag attttatcgt gatatcaagc cgaaccctgg gcatctcttg caagagacgc
     7081 ccgactatct tcggcagtat gaaagctcca atggacgtgg tagtcgcgat tttgacctcc
     7141 ccagccacgc ctccggcaag ctctcggatt gatcggccca attggtcggt ggaattgacg
     7201 atctgtcgtg ccaaggatga aaagatctcg cctgcttcag tcaaatgaat tggtcgctgg
     7261 gagcgatcca gtaattgaag gccggtgtct tgctccagta cggcaacctg ggtgcttatg
     7321 gtcgactgcg ttttgcggag cgtttgcgcg gctttcgtga agcttttcag ctcgcaaact
     7381 ttgagaaagg ttcgcaggtg ttcagtaaat acgtccatcg tattttccga tattagcatc
     7441 gcaaatatca agaaagcttg acaatggaaa gcacccttat atcgtgagcg cgaatctcga
     7501 aaatttctcc acggagtgcc tatgttgcag ggttaccgag tctacgatgc cgatgcgcat
     7561 gcgatcatga gcccggcaat gtgg
//
LOCUS       DASUVS010000130        37171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_48897, whole genome shotgun sequence.
ACCESSION   DASUVS010000130 DASUVS010000000
VERSION     DASUVS010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 37171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37171
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48897"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VF182_07325"
     CDS             complement(<1..964)
                     /locus_tag="VF182_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016861126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEX6436925.1"
                     /translation="MKTKAAIGYAAGQPLEIATVELDGPKAGEVLIEIKASGVCHTDE
                     FTRSGADPEGLFPVIFGHEGAGIVVDVGAGVTTVKKDDHVIPLYTPECRQCKSCLSRK
                     TNLCTAIRETQGKGVMPDGTSRFSIDGKMIHHYMGCSTFSNYTVLPEIAIAKIRPDAP
                     FEKVCYIGCGVTTGIGAVINTAKVEPGANVVVFGLGGIGLNVIQGARMAGANKIIGVD
                     VNPQRKQLAGKFGMTHFVNPKETGGDLVQHLVALTDGGADYSFECVGNVDLMRQALEC
                     CHRGWGVSVIIGVAGAGQEIKTRPFQLVTGRVWKGTAFGGARGRT"
     gene            complement(1258..2370)
                     /locus_tag="VF182_07330"
     CDS             complement(1258..2370)
                     /locus_tag="VF182_07330"
                     /inference="COORDINATES: protein motif:HMM:NF018439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HEX6436926.1"
                     /translation="MNQEKPIRIFQALAVLLLCFPFATHAASGDDFIRGYASAVLERE
                     FRLKDFSVNIAGESVTLISGELTQADYDRVIAALSSIDGVKHVAILDFRGVEVASSIP
                     QASGIQRATRQAVPEYELGFLPGGNLFQPLIADPRWPRFSIGYRYFTDESRNVLSATF
                     GETIALYRDRGPYDGAWEVGFQAGVFSIFDLDSESFDLVNSDFLAGLHTIYRIGDLSA
                     FFRVFHQSSHLGDEFLLRNRIDRVNLSFEGLDLKLSYRFFDWARLYGGGTYLVHRDPS
                     DLKPWSTQGGIELQTPWRFWGDSTRFVTALDVQNREENNWSTEVSVRGGLQFERAQRF
                     GRRISLLVEYYNGHSPNGQFFNRKVEYFGPGLHLDF"
     gene            complement(2474..4942)
                     /locus_tag="VF182_07335"
     CDS             complement(2474..4942)
                     /locus_tag="VF182_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEX6436927.1"
                     /translation="MWAKLTRKENTMAETLSKEQLRLLDAYWRAANYLSVGQIYLYDN
                     PLLKEALTKEHIKPRLLGHWGTTPGLNFIYAHLNRVIKARDLDMIYITGPGHGGPALV
                     ANAYLEGTYSEVYPHISADAEGMKKLFKQFSFPGGIPSHTAPETPGSINEGGELGYSL
                     SHAYGAAFDNPDLVVACVVGDGEAETGALATSWHSNKFLNPDNDGAVLPILHLNGYKI
                     ASPTVLARISHEELEQLFRGYGHTPYLVEGHEPEKMHQLMAAALDRVLDEIKQIQSDA
                     RSGKARGERPRWPMIVLRSPKGWTCPAEIDGKRTEDYWRSHQVPMGEMHSNPAHVKIL
                     ERWMKSYKPDELFDESGRLKSEIAALAPKGERRMSANPHTNGGLLLRGLRLPDFRTYE
                     VEVSEPGAETAEATRVQGRYLRDVMKLNLESKNFRLFSPDENNSNRWQDVLDVTNRAW
                     NAERFDYDDKLAPDGRVMEMLSEHQCQGWLEGYLLTGRHGFFSCYEAFIHIVDSMFNQ
                     HAKWLKVCNDIPWRRPIASLNYLLSSHVWRQDHNGFSHQDPGFIDHVVNKKAEVVRVY
                     LPPDANCLLSVTDHCLRSRNYVNVIIAGKQPAPQWLNMDQAIKHCTAGIGIWEWASND
                     RGGEPDVVMTCCGDVPTLETLAAVDLMRRHIPELKIRVINVVDLMTLQSPREHPHGLS
                     DNDFDALFTKDKPIIFAFHGYPWLIHRLTYRRTNHDNLHVRGYKEEGTTTTPFDMCVL
                     NDLDRFHLVGDVIDRLPQLGARSAYAKQAIRDALIDHKEYIYEHGEDSPEISGWTWGG
                     KITARLDSTEGDNIDTGVEAPVRN"
     gene            5220..6239
                     /locus_tag="VF182_07340"
     CDS             5220..6239
                     /locus_tag="VF182_07340"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436928.1"
                     /translation="MMGLLSLVFSLLVASAAWANPYLAKPNERPLSMRVSTCAVTGGF
                     VHLYTALDNGLFDKYGLKFENIFIRGSGPNLSALSGDEVQFTYCASDAIIPGMAAGID
                     AKIVASPLVKLPYVLVTRNDIKRSEDLKGKTLGIARAGDLSDRLSRAVVRKLGLPPEA
                     VTIRPIGGSQSERYQAMAANIVHGVIITPPLDVRAKNEGYNVLYRLIDLNLPFIYSSL
                     HTSYKMLRERPEIVQRAVAALAEIVHFVEKNPQKAKASMAKAMRTNDQEALQSAYDVY
                     AREILDRTMVVPGKAVADAVELARESGTPVRKKPEELYDNSFVNNLEKSGFLKEIWGS
                     ENYKR"
     gene            complement(6369..6695)
                     /locus_tag="VF182_07345"
     CDS             complement(6369..6695)
                     /locus_tag="VF182_07345"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6436929.1"
                     /translation="MKIVNHFAFAAALLILPYVLGCRMMRSEATVEIPTTETAQDESL
                     SKSVRDRLLADKKVDLTRVKVISRSGTVYLSGTVPSLDARQQAIKIAWNAPGVQSVVN
                     ALEVQK"
     gene            6757..7728
                     /locus_tag="VF182_07350"
     CDS             6757..7728
                     /locus_tag="VF182_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6436930.1"
                     /translation="MMEIESPLEPELPICDGHHHLWQRPPRGYMLDDLLQDLRAGHNI
                     VSTVAVECRYGYRNDCADELKPVGEVEFLESIGERVAIDATITARVAAAIVGYANLLL
                     GDAVAPVLEAHMAASPCRFRGIRHSATWDASDALRSEAARGLLGDSRFRQGFDRLAQL
                     GLSFDAWVYHPQLIEVAELARAFPSVPIILNHIGAPLGVGPYAVKRAEVYQLWRSAID
                     HLAQCPNVVVKLGGCGSLRSGYDWHERTVKPSSLELAEILQPYFEHCIEKFGVDRCLF
                     ESNFPVEKLANTYVNLWNAFKRMTQRYTPSERAALFHDTAARVYRIG"
     gene            8025..8366
                     /locus_tag="VF182_07355"
     CDS             8025..8366
                     /locus_tag="VF182_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436931.1"
                     /translation="MLIEKNFDRGYELLSDNGKRHISPAKFKETMARLHPRGFPAIVT
                     AREYQTMPGENALWIYLNGENPEMHYRLTMEASPSGDYKVLTIDSGAVGRLFSSVSDK
                     KELSEVFSTPH"
     gene            8847..10106
                     /locus_tag="VF182_07360"
     CDS             8847..10106
                     /locus_tag="VF182_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX6436932.1"
                     /translation="MNFPSEHDFGLLMFQLFIILTAALGLGELFRRFGQAQAIGEVTA
                     GILLGPSVLGALFPGHYEALFPPSQTHLLGTLAWLGSVFLLLVAGIEVDLAAIRREKR
                     VIFSTSFFSILVPFALGFSFAAELPDAYLRDPSQRLTFALFVATALSISAIPLIAKIL
                     MDLNLLKTPMGQTIVGCAVINDLVGWMFFAMILTMLTGKIGGESSVMAIAFLTLGFSL
                     VTLTAGKHLAKRLFAAFHRRGFPYEGILGLAVLVAFFCAAVTQWIGIHAIFGAFLAGV
                     MIGETGEIINRTRETLRNVSFYVFSPVFFASMGLQANFIADFDWLLVAGTITLACIGK
                     VSGATVGAMVAGRPRSEALTIGFGLLPQGAMGMILAFLALQHGLISDPIFVALLLAAI
                     ATSVLAGPLIQTMAPTLKRSLSTSEEH"
     gene            complement(10247..10651)
                     /locus_tag="VF182_07365"
     CDS             complement(10247..10651)
                     /locus_tag="VF182_07365"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6436933.1"
                     /translation="MIKRETVMAPAGGPSHGKGMVQGIKAGGFIFFSAIRGNHPLTNY
                     ESDDTKEQARQAFENLKVLLAAAGAGLEHVVKVTLYLQDLKYRLLFHDVWMEYFPKDP
                     PARIAIQVADANAQPGRNAHFALDVIALDPNA"
     gene            complement(10905..11330)
                     /locus_tag="VF182_07370"
     CDS             complement(10905..11330)
                     /locus_tag="VF182_07370"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6436934.1"
                     /translation="MANANKIIVQPKDLPKRGTHSQVVAVTGGTLVYIAGMTSRSEKD
                     ALPVHAGDMRAQVRQICENLGRALHSVGGEFSDIVKTTIFTTDVEEFRKANEVRYEYF
                     TSDYPTGSLIGVKRLAHPDLMVEIEAFAVVPADRFKPAQ"
     gene            11518..12540
                     /locus_tag="VF182_07375"
     CDS             11518..12540
                     /locus_tag="VF182_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6436935.1"
                     /translation="MKVLAALALLLVSIVSTRNSELNAATVDQVIASAKKEGSLEFYA
                     PGTLTAQGAKQLEDAFNKMFSSDIRINYSQSGSMTADVSKYIGEAAAGNPPQWDVMVL
                     IDALHGSIWKRKLHIPFDYRVLGVNQRAIHYNNGTVSFANQVVLPAYNSKIVDPKDVP
                     RRWEDLLDPKWKGKLGMVTGVGHIDYLAMRWGEKKFLDFGRALAKQNPVPGKLGELYT
                     RLQIGEISLTTTLTNSFIVRAQKEGAPLEHAQVQPVVAPAYHIGVLKGSRHPNTAHLF
                     ASFLLSLEAQAIWERYNGQTSAFVPGTKMYNYVQGKDVIYLGQDDVDLVNRAVVEVNK
                     ILGFNP"
     gene            12564..12986
                     /locus_tag="VF182_07380"
     CDS             12564..12986
                     /locus_tag="VF182_07380"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6436936.1"
                     /translation="MTNPKRIVQPKDLPIRKSHSQVVTVTGGTLVFIAGMTSRSEVDA
                     TPVHRGDMRAQLRQCCENIGRALRAVGGDYSDVVKTTTFTTDVEEYHRVNDERAKYFK
                     SDLPTSALIGVVRLAHPDLMVELEVMAVIPTERFNPQG"
     gene            complement(13081..13986)
                     /locus_tag="VF182_07385"
     CDS             complement(13081..13986)
                     /locus_tag="VF182_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436937.1"
                     /translation="MKTLRLETTAPFQGLAELVAYDEGLFEKEGVQIEWMDREKGLDK
                     SPQLHITDHKDASRYTSHGRMMESGQADLYNACEWGNFSRVETTNVGSRQVGRRSMVN
                     FAAIVVRADSPVQVPQQFADVQIGVPFYSGTHYLCLQMLEGFVPRELIKIGRVPNGSN
                     YRFKMLMDKTIEATTLTEPYISLAEKMGCRLVISAFHHGTDVASDRVDAETYAAFNRA
                     IREAVRRIMANKRAYMHYFIDYYRDRDPQINQLTVDDLRESRLYLVDPAPIPADELQR
                     TYDWMKSWDFLETAPCATDLVDMNV"
     gene            14446..14973
                     /locus_tag="VF182_07390"
     CDS             14446..14973
                     /locus_tag="VF182_07390"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX6436938.1"
                     /translation="MGAEETKHDLWLKRINTWIAILGGSVASIAGTYNFLPSFFPPAP
                     GHIAAVVREQGGAPVPRAHVELLNSENVLLVASETDRDGRFSRKDLEAGSYLVKVSRA
                     SFEPQTAKVSIASKKTTDLDLVLKSRPRAQVSNSAPPNTPQPRAISQPEGSAIRSALE
                     ETGAAWIKNLSNAGR"
     gene            15545..16300
                     /locus_tag="VF182_07395"
     CDS             15545..16300
                     /locus_tag="VF182_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6436939.1"
                     /translation="MREAQQISRRTWLVRFALGSFALWNELNFGFGRRGWGIAIGGGD
                     LAVGVAHAQNLEPANALRVNLGIVNAYVLIRGKEAAVVDTGTPNNGSKIADVVRTAGL
                     SWNAVHHVILTHYHPDHIGSVGEILAAAGKATAYAGAGDVDQIKSPRPLKAVNDNDEV
                     FGLRVIATPGHTPGHICVFDPAGSLLILGDAMNNIGNKLAGPNPQYSADMAQANQSVK
                     KLAKLKFQRAVFGHGEPIDNGASQAITKLAGTL"
     gene            complement(16508..17734)
                     /locus_tag="VF182_07400"
     CDS             complement(16508..17734)
                     /locus_tag="VF182_07400"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436940.1"
                     /translation="MISGFVWWPHIWRNTFLETYLRCHVVARCKPCLMVPLQNIMETK
                     IDLHSFADHGTMKREDKTSTQENGRFLQPSAILVLTFCLCLAGPPLPSLWGAQPPAKP
                     SVAYGVISNAVAPLWVAQEQGLFRKYGLDPDLVFIIAGTATQAMLAGQVPFGLLAITH
                     VANAVVAGGDLTMVLSFFNTLDYLFVSQASIKSVEALKGKRVAIGTPSGLPGLMTFMV
                     LDHFGLNPKRDNIVLSQIGSVPARMAALRAGSVEATSLPPELSQEIAREGFNVLFDAA
                     KESIPFQGTGLVMSRKLMKSNPQLAENMVRALTEAVAFIHIPSNRKTVEGILAKYMKL
                     NKPELIQEAYQSMLKALPRKPCPTLKGTALVLKLMAQYGINPKADQLQTDDVVDMSLC
                     TRMDESGFIDRLYQGR"
     gene            complement(17975..19018)
                     /locus_tag="VF182_07405"
     CDS             complement(17975..19018)
                     /locus_tag="VF182_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436941.1"
                     /translation="MAVEQFKQFKKTSVILLFSFCLWLAARPLTSVWAAQPPAKPSVA
                     YGVISNAVAPLWVAQEQGLFRKYALDPDLVFIIAGTATQAMLAGQVPFGLLAINNVVN
                     AVSGGGDLAMVLGFLNTLDYLFVARSSIKSVEELKGKRVAIGTPSGVPVLMTYMVLDR
                     YGLNPKRDNIVLSQIGSVPARMAALRAGSVEATSLPPELSQEIAREGFNVLFDAAKEN
                     VPYQSTGLVMSRKLKQSHPQFVENMAKALIEAAAFIHNPSNKKQVEVTLAKYLKLNRP
                     ELVEQAYQTLLTALPRKPCPSTKGTALVLKLMGQYGINPKAAQLVPDDVLDMSLCQKL
                     EESGFFERLYQGR"
     gene            complement(19184..19633)
                     /locus_tag="VF182_07410"
     CDS             complement(19184..19633)
                     /locus_tag="VF182_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436942.1"
                     /translation="MTKQLCFEDVDLGYAIPKRAFGPLTIVDTVQWAGFQENWYRLHW
                     DRDFVRERSGLTTFIASGGYREALLVRTITDWIGLRGILRRLTVRQSFPTFDGDLIVY
                     SGTVAEKSPTPTDPWVACELQGANQADRILLNARCHVTLPSILLDKK"
     gene            complement(19648..20772)
                     /locus_tag="VF182_07415"
     CDS             complement(19648..20772)
                     /locus_tag="VF182_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6436943.1"
                     /translation="MSTIIDLTDVSAAYGTRLLAEAGHEVIRIEPRSGDRLRRLGPLV
                     TENPDLEHGPYHMFLNMGKKSVTLDLKNQTGCKVLLDLVRICDVLVSNNPLPVDETVL
                     RQTNPRLVVTVLEDELPEICAYARSGLMYITGQPSERPVLAGAHVAHVIVGLYVAIAT
                     AAAMNMQDLLGEGDVVRVSAQQSLESLMEQAVIAFTSSGEITHRRGLRGQITAISGAL
                     ECADGHWMVSVPPNPAAWTKIKEWVGDPALTMDPALDDDTGRNQKRDFILDRLEAWSK
                     QFSKEYLVTGAQSRHMAASPVATPVELVRDPQLLERGFLRDIDHREFGRITVPGGAIS
                     TARHSELHQAPSLGQHTAEIMNRLGYSKYDQQALLESGVI"
     gene            complement(20775..21311)
                     /locus_tag="VF182_07420"
     CDS             complement(20775..21311)
                     /locus_tag="VF182_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6436944.1"
                     /translation="MKDEVNFLISDELKTWCGRTIGPIPLPEPISASDLRRYINATGD
                     RNPLWEDDEIARSAGYRSRVVPPAMIAELYRRNEGSKGTHSDNLWEAVPLPKGYTESR
                     NAQAEFEWFEPVYIGDRLNVTHRIIDIVARRTRSGVGIFVTREMIFRQEKGNLVLLVR
                     QTSVKLPKRNAPVNEGES"
     gene            complement(21319..22512)
                     /locus_tag="VF182_07425"
     CDS             complement(21319..22512)
                     /locus_tag="VF182_07425"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6436945.1"
                     /translation="MQGLRVLDFGHIVAAPFCTRILADLGADVVKIETSLRKDFAGGT
                     RTKPAGSEPDERPPAYLNINRNKRSLTINLKTDAGRDLAARMAAVADVLVENFSAGVM
                     ERLGLGPHYLQTQNPRLIFVSMSGYGHSGPRRNWRSMNLNLQAYSGLMTATGNDGEAP
                     VAISNSWNDFVGGLHACFGILHAMSERNQSGLGTYLDVSQFECSVASVGALLFAASLT
                     HQNPPRIGNRSMSAAPHGCYRCAGEDKWCVIAVESDEQWLALVKALGNPLWASDAKFQ
                     NLLGRMQHQDEIDAHLEQWTSELQNTDVERLLQSVGVPAQRVRRIDEIVKSRDERSIF
                     GRMDHPPQAPRLVAKVPFIYSRYSLASPTPAPRLGEHNRDILRQWVSLSDEEIRQLEN
                     QGALE"
     gene            complement(22539..23603)
                     /locus_tag="VF182_07430"
     CDS             complement(22539..23603)
                     /locus_tag="VF182_07430"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6436946.1"
                     /translation="MIKPPRNKAAALAIAWLLLLLNFFSPTTLHAQQEPYYKGKTIRI
                     VVGLSAGGFYDLWPRLVVKYLGKYIPGNPTFVVQNMPGAGSIIAANYVYNVAKPDGLT
                     LGAPHAGIYSAQLSSQKEVQFDIRKFHWIGSPVTNPLILYMRADAPYKTIEDLLKAKE
                     PAKCGGSGVASSDYILAKVLQEALGVKIQTVLGYAGGTEIDLAVEKGEVHCRGMIFPA
                     HFGREPFTSWHKNNFDLHILQTGKTRDSRSPDAPTIYEIMDKYKTSDASRRVVRVVLL
                     GSDFGQPIVVPPGTPAELIKVLRSGYAAALKDPDLLADAKKARMDVDFVSGEELERLA
                     QEAINQPADVVERVKKILTQ"
     gene            complement(23664..24080)
                     /locus_tag="VF182_07435"
     CDS             complement(23664..24080)
                     /locus_tag="VF182_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX6436947.1"
                     /translation="MSEYRKPLPKPAVESEPFWHGCKKHQLLLPHCPQCGSYWFPASV
                     TCPQCLSLHWEWKPSPGKGKIHSFGVYHRVYHKGFEPEIPYAVALVQLDEGPRLVSNI
                     VGCTLEELRCDAPVEVVFEDVTENMTLYKFRLVGNL"
     gene            complement(24077..25255)
                     /locus_tag="VF182_07440"
     CDS             complement(24077..25255)
                     /locus_tag="VF182_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX6436948.1"
                     /translation="MSEKFRGKYAIVGVGQSPLGKIPEYGDIGLFAIAAKNAIEDAGL
                     KRADVDGLISHGPDDSYTHHQHVGEALGINASFSTSTDNGGASQVLGVAMACMAIDAG
                     LCNTVVVGYARDAWSRTHRNAEAKALVAANSEAQALKEFGPEFGLFGQPAHYAIAARR
                     HMQLFGTKKEQLGSIAVAFRNHARKNPNAFMRDPLTLEQYMKARMIVDPLGLYDCSVY
                     VDAAGAVVVTSAERARDLKKPPVYVLGFGFGNRLKGWFADDNMITTGAKEAGETAYRM
                     AGVGPKDIDTAQMYDCFTHMVLLQLEDYGFCKKGEGGPFAASGALEFDGELPTNTSGG
                     QLSEGHVQGMLQICEGVRQIRHEHGPERQVKDAQIALVTGHGGNTVCQSAVILAREAS
                     "
     gene            complement(25291..27714)
                     /locus_tag="VF182_07445"
     CDS             complement(25291..27714)
                     /locus_tag="VF182_07445"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6436949.1"
                     /translation="MERPLVPGEVAENMESRGAFLKGYRALDLTDLRGQLCGRVLAGL
                     GMEVIKIEPPGGDPVRELAPFIGSADGRRLSSTFAHLNAGKASRILDLKKKRDRDALL
                     ELVESADVLLESFHPGELAAIGLGYERLTKSNPGIVMASITGFGQTGPKSKLACNDLV
                     AMAESGFLYISGTPELPPCKPPEAQAYYFASLYATVGVLAALYSRERTGEGDHLDVSM
                     QECLATQEHIIRLWANEKEVLRRAGSQHASVAPAKIFRCRDGYVYLYVTRQHWKLLLD
                     LWKDHPPHFDGPEWLSYVHRRMHVEDLNPGVEAFLSKYRVTEITELLQSQGIPCVPVN
                     TPLDFINDQHVQSRAFIVSVDHPGFGNAKQTAMPFLIDGVRPPVGSVPVLDSWQDDGI
                     KRSPAPTCNPSVPRGNGPLHGMRIVSFDHVLAGPYGTTILAELGADVIKVESRKGGLD
                     PFRFFGEAGDPNVSPRFLEFNRNKRSFTVNLKHPSGHKIVHDLVRKADAVMDNYSVDV
                     VERIGLGYEQLCKIKPDIVNLRMPGLGTTGPKRHFATLGVNIAAFTGLTYLWNHPGNT
                     APPIGSQTVLPDYVSGVLSAILIVSGVLHRDRRKTGAFIDLAQAEATAFMIGANLMDA
                     VANGSNPEPTGNASLSSAPHDCYPCAGEDRWCVIAADDDQQWSALARIVGGGLEQDGR
                     FTTSADRLKNRDTLNMIIAQWTRDEDAFAIRNRLQQSGVPAGVVQTGRDLVDDPHLKE
                     RGFIVAVENPRLGRVVLPNFPLQFAKAKLSRRWEFPELGRDTEAVLRELVGYSTTTIA
                     AYKQDGALD"
     gene            complement(27699..28499)
                     /locus_tag="VF182_07450"
     CDS             complement(27699..28499)
                     /locus_tag="VF182_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014629251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6436950.1"
                     /translation="MSYQAIVYDVRNHIGFVTLNRPQVLNAVNQQMRREIIDACERMR
                     KDPEVRACIFTGSGERAFSTGFDLKEGAAQSDDQSLFAQRHGRRQPGIHFHHQAISSM
                     DKPTIAAIRGYAVGGGLEVALACDLRVAAEDAMLGMMEVRRGRLGGSGGTQRLPRLIG
                     TANALELCLTGELVNAAEAFRLGLVNRVVPVDRLMAVAEDIATKICLGAPLSVIAIKE
                     AITKGVELPIDQGLQLESELGLALSTTEDQKEGARAFAEKRPPVWKGR"
     gene            28711..29601
                     /locus_tag="VF182_07455"
     CDS             28711..29601
                     /locus_tag="VF182_07455"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX6436951.1"
                     /translation="MDFKTLNAFYLTISTGSTLRAASLMELTVPTLSVKLKNLEKDLK
                     VRLFDRLPNKLVPTPEGMAFYGEVRQVLECIERAKTAVHRKQGHSFEGRISISMGSDL
                     IQIFARRLGSFLKRHPRLECTLLARASSETFSLVLAGNVDIGIGRFDSMPRALIAKDL
                     FENSIWCSFPRKHPFARDGRISVGDFESHRIITLARSSGTGRAIDQVFRRSRVRPQNI
                     LEVGSCRSALEFVKLGVGIGLVHNNCLITERKDNLRFKDVSHIFGAAPVTLIYKRSTI
                     LTPAHLGIMDIFDHGPRGHL"
     gene            29935..31038
                     /locus_tag="VF182_07460"
     CDS             29935..31038
                     /locus_tag="VF182_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cj0069 family protein"
                     /protein_id="HEX6436952.1"
                     /translation="MAQNSDVRLAILSPGDRAARQNTRGETSRFADLFREFAVRGIHV
                     EAAIYHDDFCEEVREQLMRVDGILVWVNPIEGGRNRSMLDSMLRDVAAAGVFVSAHPD
                     VILKLGTKEVVYRTRNLGWGCDTDLYASFDQMVQKLPLRLATGRARVLKQHRGNGGNG
                     VWKVQLARDELADRTGAASAALPQPETVIQVRHALRGCSEEEIKLGDFYHRCAPYFYA
                     NGPMIDQEYQERLAEGMIRCYLVHDKVVGFGHQAINALFPAPPGAPSTEAPPPGPRLY
                     HPPSMPEFQPLKVKLEQEWVPAAQRLLDIETEDLPILWDCDFLLGSKRDSGEDRYVLC
                     EINVSSVAPYPESAVPYIVDATVARLRATRQRR"
     gene            complement(31297..32532)
                     /locus_tag="VF182_07465"
     CDS             complement(31297..32532)
                     /locus_tag="VF182_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6436953.1"
                     /translation="MQRAQLIAVLAVITFGLIAGCAVPVSEQPVTAYERARLIVGDGR
                     VIENATLIVQDAKLAQAGRAADVRVPAGAKRVDLAGKTVMPMIVDTHVHLSDSRDAVI
                     RDLRMRAYYGVSAVLSLGTDGYEVLDLHSQTIDGAARYRSAGRGITMPEPGRTKVPYW
                     VTSVAEGRKAVEELSAHRVDIVKIWVDDRDGKYKKLTPEIYGAIIDEAHKRKLQVIAH
                     IFELADAKGLIRAGIDAFAHGVRDRDIDDELVTMFKQRPNLVLTPNLPDRGVKADLSW
                     LRASLPADEFKKLEAANTDRPKVQALYGIQARNLAKLNAAGVRIVLGTDGNRPWGPHE
                     EMLDMVMAGMTPAQVIVAATRNSADFLRLTDAGTLEAGKSADFIVLDANPLDDITNTR
                     RISAVILRGTAVDRAQPVR"
     gene            complement(32840..33601)
                     /locus_tag="VF182_07470"
     CDS             complement(32840..33601)
                     /locus_tag="VF182_07470"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436954.1"
                     /translation="MADYVKKLLPRFRYGVIQPRAHEEVQRARSYQLYRLLPLDFMEI
                     STGLGLENYTKEGVEKAIKNYWICIDRLVKEKVDHIIFSGAPISAMLTRPKVRELLRE
                     TKEKTGISADAPLEALIAAMNHLGLTKLAIGSRWADPVNEAIKRYLEAGGINVVGITK
                     RNQWASDSFAMTLEQGLEMALDVGREAARTWPDAEAISIPGGAAMSLHVIPAIEEEYG
                     KPAFTNMSAEVWNDLVRPGIISPVQGWGRLLAGSK"
     gene            complement(33662..34711)
                     /locus_tag="VF182_07475"
     CDS             complement(33662..34711)
                     /locus_tag="VF182_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436955.1"
                     /translation="MTKTRLRAHLHWLPQGVNGLLLAALVISLLWPQTSSALADRAKP
                     LVKVVIGYGSTDGGVAVLGFAKEAKLFEKQGLDVVLVGMGTGSVSLRALIAKDLQISS
                     LSGSGLVQAAMQGADTVLIASLINGFVFKIFSAPEISSPAQLKGKKLGVSRYGATSDF
                     AVRLALKKWGLNPERDVNILQIGTSQDTVRALQTKMLDAGVTTGTSSLIARKAGFREL
                     ADLADLGLDYPTSAIGTTRSYLQKNEATVKGFMLAYIEAIHGFKRNKEAALAVLRRYT
                     RTDDREVLEHNYDEIASRYQPLPIPTLEGIRTILTELSSTTPAAKKADPEQFVSYKIA
                     REIEASGFVKGLYEK"
     gene            complement(34947..35906)
                     /locus_tag="VF182_07480"
     CDS             complement(34947..35906)
                     /locus_tag="VF182_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436956.1"
                     /translation="MRRCFAYVFLLLFGFLVHPSVHAADRIRIGFPDLAAPFVPLAIG
                     DKRGFFQEEGIVAESIRMNPAVALQALVGGEIDYYTVLGPGVAAAIRGVPVKLVAAYV
                     PISPTALIARPEMKSVTDLKGKTIGINAYGGALEAMGRLIMKHFGIDPDKEVKFLATG
                     ALDSRFGAMKQGLTFATLGGPPIDFLGETLGFIVLARAHELFSFPVSGLIATEKKIKE
                     RPDEIGRVIKAGIKANRYIHQSRDGTLPIMAQWLKIDKAMAARTYDSSVKTFSEDLAL
                     PEDGLRLLIDDVKRTAKVNREIAISDVADLSILKMAQRELRTR"
     gene            complement(36022..36333)
                     /locus_tag="VF182_07485"
     CDS             complement(36022..36333)
                     /locus_tag="VF182_07485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX6436957.1"
                     /translation="MVILHVTVQVKPEHVPEYLEAVRHDAEHSERDEPGCLRFDVIQD
                     RDDANRFYYYEVYRDEAALEAHRQSPHFKLYVEKTSAWLAAAPERRFGKNLIPADSQW
                     R"
     gene            36585..36860
                     /locus_tag="VF182_07490"
     CDS             36585..36860
                     /locus_tag="VF182_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436958.1"
                     /translation="MNLPSVENLVTASHILADQGVLDGLGHISARHPDNPQGYLMPRS
                     LAPAFVTLQTSSNTTSIQTLSIDRTDRFSSNVLFMEKPTRFGRTFSQ"
BASE COUNT         8327 a        10450 c         9842 g         8552 t
ORIGIN      
        1 cggtgcggcc gcgcgcaccg ccgaatgcgg tgcctttcca cactcgcccg gtgacgagct
       61 gaaacggccg cgttttaatt tcctggcccg ctcccgccac cccgataatg accgagacgc
      121 cccagccgcg atggcagcac tccagggcct ggcgcatgag atcgacattg ccaacacact
      181 caaagctata gtccgctcct ccgtcggtga gcgcaaccag atgttgcacg agatcgccgc
      241 cagtctcctt cggattgacg aaatgggtca tgccgaattt tcccgccaat tgcttgcgct
      301 gcgggtttac gtccacaccg atgatcttgt tcgcgccggc catgcgcgcg ccctgaatga
      361 cgttcaagcc gataccgccg aggccgaaga ccaccacgtt ggcaccgggc tcgactttgg
      421 cggtgttgat caccgcgccg atgccggtgg tcacgccgca gccgatgtag cagacttttt
      481 cgaaaggcgc gtccgggcgg atcttcgcaa tggcgatctc gggcagcacg gtgtaattcg
      541 agaacgtcga gcagcccatg tagtgatgga tcattttgcc gtcaatggaa aaccggctgg
      601 tgccgtccgg catcacgccc ttgccctggg tctcgcggat cgcggtgcag agattggtct
      661 tgcgcgacag gcacgatttg cactggcggc attcgggcgt gtaaagcgga atgacgtgat
      721 cgtctttctt gacggtcgtc acgcccgctc caacatctac gacgatgccc gcaccttcgt
      781 gcccgaaaat caccgggaaa agcccctcgg ggtcggcgcc ggagcgcgtg aactcatcgg
      841 tgtggcacac gccggacgct ttgatttcga tcagcacctc gccggccttg ggtccgtcga
      901 gttccaccgt cgcgatttcg agcggctgcc cggccgcata cccaatcgct gctttggttt
      961 tcatctcgcg tattcctccc tatacgaaac ggcaaaccaa cacacccgcc atcactgaag
     1021 tctagcgcct cgcatcaagc cgagatttgt acgctagcgc gaacgaatct aaagagccag
     1081 cggcgcaccg cgcctatggc aacgaagatc acgattgaca ttcgggaacg gcaagagtca
     1141 ttgacatgtt tcgcgcgatg acggcaacgt cacggctatg cattaattac tgaaagacaa
     1201 acttctggaa atgctcggcg ccgtcgcgcc cctcatataa tggcataggg tgcgacacta
     1261 aaaatccagg tgtagccccg gtccgaaata ttcaaccttt ctgttaaaaa actggccgtt
     1321 gggagaatgt ccgttataat attcaaccag caaactgatt ctccggccaa atctctgagc
     1381 cctttcaaat tgaaggccgc cacggaccga gacttccgtg ctccaattgt tttcttcccg
     1441 gttttgcaca tccagagccg taacgaagcg ggttgagtct ccccaaaacc gccacggcgt
     1501 ctgcagttcg ataccgccct gtgttgacca gggcttaaga tccgagggat ctcggtgaac
     1561 caggtaagtg ccgccgccat ataggcgcgc ccagtcgaaa aacctgtaag atagtttgag
     1621 gtccaggcct tcgaaactta gattgactcg atcgattcta ttgcgcagaa ggaactcgtc
     1681 gcctagatgc gagctctggt ggaaaacgcg aaaaaaagcc gacagatccc cgattcgata
     1741 gatggtatgc agcccggcta aaaagtcgct gttgaccaga tcgaaggatt ccgagtccag
     1801 gtcaaagatc gaaaatacgc cggcctgaaa gccgacctcc catgctccat cgtaagggcc
     1861 gcgatcgcgg taaagggcaa tggtttcacc aaacgtcgcc gacaacacat tgcgcgattc
     1921 atcggtaaaa taccggtagc cgatcgagaa gcgcggccag cgcggatcgg caatcagcgg
     1981 ctggaaaaga ttgccgcctg gaagaaaacc cagctcgtat tcgggtacgg cttgtcgcgt
     2041 ggcacgctgg attccagatg cttgcgggat cgatgaggca acttcaaccc cgcggaaatc
     2101 caaaatggcg acgtgcttaa ctccatcaat ggatgataaa gccgcgatga ctctgtcgta
     2161 atccgcttga gtcagctctc cggagatcaa cgtgacggat tcgccggcaa tgttcacgga
     2221 gaaatccttt aagcgaaatt ctctctccaa cacggcggac gcatagccac gaatgaaatc
     2281 atcgccactg gccgcgtgtg ttgcaaaagg aaaacacagg agcagaaccg ctagagcctg
     2341 gaagattctt attggctttt cctgattcat ggcgttctcc tggatttacc ggaaatgtcc
     2401 ttctaatcga ttgacgttct ttgttcttcg tcgagcaata gtgagcaatc acttaagaat
     2461 ctcgctcgtc cgattaattt cgaacaggcg cttcgacccc tgtgtcgatg ttgtcgcctt
     2521 cggtggagtc aaggcgggcc gtaattttgc ctccccaggt ccagccgctg atctccgggg
     2581 agtcctcgcc atgctcgtaa atgtattcct tatgatcgat gagcgcgtcg cggatggctt
     2641 gcttggcata ggccgagcgc gcgcccagct gcggcagccg atcgatcacg tctccgacga
     2701 ggtggaaacg atccagatcg ttgagcacgc acatgtcgaa cggcgtagtc gtggtccctt
     2761 cttccttgta gccgcgcacg tgcaaattgt cgtgattggt gcgccggtaa gtcaggcgat
     2821 ggatgagcca cggatagcca tggaaagcaa agatgatcgg tttgtctttt gtaaacaggg
     2881 cgtcgaagtc attgtcgctc aacccgtgcg ggtgctcgcg gggcgattgt aacgtcatca
     2941 gatcgaccac attgatgacc ctgattttca actccggaat gtgccggcgc atcaggtcca
     3001 ccgcggcgag agtctccagt gtcggcacgt cgccgcaaca ggtcatcacc acgtcaggct
     3061 cacccccgcg atcgttgctc gcccactccc agatgccgat gccggcggtg cagtgcttga
     3121 ttgcttgatc catattcagc cactgcggag cgggctgctt gccggcaata atgacgttga
     3181 cataattgcg gctacggagg cagtgatcgg ttaccgaaag caggcagttg gcgtcgggcg
     3241 gcagatagac gcgcacgacc tccgcttttt tattgaccac atgatcgatg aagccgggat
     3301 cctgatggct aaaaccatta tggtcctgcc gccacacatg agaggacagc aagtaattga
     3361 gcgatgcgat gggcctgcgc caggggatgt cattacatac ctttaaccat ttggcgtgct
     3421 ggttgaacat tgagtcgacg atgtgaatga aagcttcgta acaggagaag aagccatgcc
     3481 ggcccgtcag aaggtagcct tcgagccagc cctggcactg gtgctcgctc agcatctcca
     3541 tgacgcggcc gtcgggcgcc agcttgtcat cataatcgaa acgctccgcg ttccaggcgc
     3601 ggttagtcac atcgagcacg tcttgccaac ggtttgagtt attttcgtcc gggctgaata
     3661 ggcgaaaatt cttactctca agattgagct tcatcacgtc acgcaggtag cggccctgaa
     3721 cccgggtggc ctcagctgtt tccgcccccg gctccgatac ttctacttcg taggttcgaa
     3781 agtccggcag tcgcaggcct ctgagcaaca ggccaccgtt ggtgtgcgga ttggcgctca
     3841 tgcgccgctc gccttttggc gcgagcgcgg cgatttcgct tttcaatcgt ccactctcat
     3901 cgaacagctc atcgggcttg taactcttca tccatcgttc gagaatcttt acgtgagcgg
     3961 gattgctgtg catctcgccc atgggtacct gatgcgagcg ccagtaatct tcagtgcgct
     4021 tgccatcgat ttccgcggga caagtccatc cttttgggct gcgcagcacg atcatcggcc
     4081 agcgcggccg ctcccctcgc gccttgccgc tcctcgcatc gctctggatc tgtttgatct
     4141 catcgagcac ccggtcgagg gcggcggcca tcagctgatg catcttttcc ggttcatgtc
     4201 cctcaacaag gtagggtgtg tggccatagc cgcgaaacag ctgctccaac tcctcgtgac
     4261 ttattcgcgc caataccgtg ggactggcga tcttgtaacc attcaagtgc aaaatgggca
     4321 acaccgcgcc gtcgttgtcc ggattcaaaa atttgttcga gtgccagctc gtcgccaacg
     4381 cgccggtttc cgcttctccg tcaccgacca cgcaagcgac caccagatca ggattgtcga
     4441 aggcagcgcc ataagcgtgc gacagggaat agcccaattc accaccctca ttaatcgacc
     4501 cgggagtttc cggcgcggta tgactgggta tgccgcctgg aaacgagaac tgtttgaaca
     4561 gtttcttcat cccttcggca tcggctgaga tgtgtggata gacttcgctg taggttccct
     4621 ccaaataagc attagcgacc aaagcgggcc cgccatgccc gggacccgtg atgtaaatca
     4681 tgtccaggtc gcgcgccttg atgacccggt tgagatgagc gtagataaag ttcaggcccg
     4741 gcgtggttcc ccagtgaccc aacagacgcg gcttgatgtg ctctttggtg agcgcttcct
     4801 tcaacagcgg attgtcgtac aaataaatct gtcccaccga taaataattc gctgcgcgcc
     4861 agtaggcatc cagcagcctc aactgctcct tggatagtgt ttccgccata gtgttctcct
     4921 ttctagttaa cttagcccac agagccgtca gcgcaatgga tgttttttta aattgttaga
     4981 cgatccaacc aagattctgc tgccggaccc cgccaaggcg ctccacttcg cgagtaatga
     5041 ccttcatcgc atcgttatca tctgtgatgc ctggagattc atcgccggat aatttttatg
     5101 tgggagattc ggaagcggaa tcgagcttca gttgccgggc aatgcggcag gcatcttcga
     5161 tagaacgcga ggcgttgctg tttacaagca gccgcaatcg ggagtagtat ccggcgcaaa
     5221 tgatgggact tttatcgttg gtattttcgc ttctggtcgc ctccgctgca tgggccaatc
     5281 cttatctggc aaagcccaac gaacggccac tctcgatgag agtctccact tgcgccgtga
     5341 ccggcggttt cgtgcatctc tataccgcgc tcgacaacgg tttgttcgat aagtacggcc
     5401 tgaagttcga gaatatattt atccgcggca gcggccccaa cctctcggcg ctgtccggag
     5461 atgaggtcca gttcacctat tgcgcttccg atgcgattat tcccggaatg gcggccggca
     5521 tcgatgcgaa aatcgtcgcc tcgcctttgg tcaagctgcc ctatgtgctg gtcactcgaa
     5581 acgacatcaa acgctcggag gacctcaaag gaaagacttt aggcattgcg cgggccggag
     5641 atttgagcga ccggctctcg cgcgcggtgg tgagaaaact cggcttgccg cccgaagcag
     5701 tcacgatcag gcccatcggc ggcagccaga gcgagcgtta tcaggcgatg gccgcaaata
     5761 tcgtccacgg cgtcatcatc accccgccgc tcgatgtccg cgccaaaaat gaaggctaca
     5821 atgtgcttta tcgcttgatc gatctcaatc tgccgtttat ctacagctcc cttcacacca
     5881 gttacaaaat gctgcgcgag cgtcccgaaa tcgtccaacg ggcggttgct gcgctggccg
     5941 agattgtcca tttcgtcgag aagaatccgc agaaggccaa agcatcgatg gcgaaggcga
     6001 tgcgcaccaa cgatcaagaa gcgctgcaat ccgcttacga tgtctatgcc agggaaattc
     6061 tcgatcgcac catggtggta cccggcaaag cggtcgccga cgcggtcgag ctcgcccgcg
     6121 aaagcggcac tccggtcaga aagaaacccg aagagctcta cgacaacagc ttcgtgaaca
     6181 atctggaaaa gagcgggttt ttaaaagaga tctggggtag cgagaactac aagcgctgat
     6241 caggccaatc tgacgtccgc cgcgcatcgt aggttacttg tttcacttgg tagaagttga
     6301 cgtaatggtt ctgctttgct gatgactcgg agtatcatta ctgctccgtg aatcgccggc
     6361 atcggttctc acttttggac ttcaagcgcg ttaacgacgc tttgcacacc gggcgcattc
     6421 cacgcgatct tgatggcctg ctgacgtgca tccaacgacg gcaccgttcc gctcagataa
     6481 actgtgccgc tgcgcgatat caccttgacg cgggtcaaat ccactttctt atccgccaga
     6541 agtcgatccc ggaccgactt agacagcgat tcgtcttgcg cggtttccgt tgtcgggatt
     6601 tcgacggtag cttcgcttct catcattcga caacccaaaa catacggcag aattaacaag
     6661 gcagccgcga acgcaaagtg attgacgatt ttcatagttc atctttatca caacgcagag
     6721 attttgcgca gctttaggaa taaaaaaaca acttcgatga tggaaataga atcgccattg
     6781 gaacctgagc tgccgatctg cgacgggcat catcatcttt ggcagcggcc gccgcgcggc
     6841 tacatgctcg acgatttgct gcaagatttg cgcgccgggc ataatattgt ttccacggtg
     6901 gccgtggaat gccgctatgg ttaccgcaat gactgcgcgg atgaattaaa gccagtgggc
     6961 gaagtcgaat ttctcgaaag cataggcgag cgagttgcca tcgatgcaac gatcacggct
     7021 cgcgttgcgg ccgcaatcgt cggatatgca aatcttcttc tcggcgacgc cgtcgcgccg
     7081 gtgctcgaag cgcacatggc cgccagtcca tgccggtttc gcggcattcg tcattcggcg
     7141 acatgggatg cgagcgatgc gttgcgcagc gaagcggcgc gagggctact tggggatagc
     7201 agattccgcc aggggttcga ccggctggca cagttgggcc tcagctttga tgcgtgggtc
     7261 tatcacccac agctaatcga ggttgcggag cttgcgcgag cctttccgtc tgtgccgatc
     7321 atactgaatc atatcggcgc gccgcttggg gtgggtcctt atgccgtcaa gcgcgctgaa
     7381 gtctatcaac tctggcggag cgccatcgac catctggcgc aatgccccaa cgtggtggtg
     7441 aagttaggcg gctgcggttc gctgcgttcc ggttacgact ggcatgagcg gacggtcaag
     7501 ccatcatcac tagagcttgc ggaaattcta caaccgtact tcgagcattg catcgagaaa
     7561 ttcggtgttg atcgttgcct attcgagagc aatttcccgg tggaaaaact tgccaacaca
     7621 tatgtcaatc tttggaacgc cttcaagcgc atgacgcaac ggtacacgcc cagtgaacga
     7681 gcagcgctat ttcatgacac cgccgcgcgg gtttaccgca ttggctaatc cctagagtcg
     7741 tgtttaatag actaggctgc agaagttcgt cggttcacgt gtcgctcgat aaaaatgaac
     7801 tcaaccgagg cgcacctcgt cagccatact ccgggactta ccataatccg cccaatgtag
     7861 tatcgaaacc gcacgactgg gccaaataaa tgaatctcaa acgatcgatc gttcgcgcat
     7921 tgacgggatg cctactcaca gtattctggc ttatcgcttg cgctgaatcg attaagcagg
     7981 acgagaatct cgccgctcga cgcgccatcg aattcgcacg catcgtgctg atcgaaaaga
     8041 acttcgatcg agggtacgag ttactttccg ataacggcaa gcgccatatc tcgccggcaa
     8101 aatttaaaga aacgatggcg cgcctgcatc cgcgcggttt ccccgccatc gtgacggcaa
     8161 gggagtatca aacgatgccc ggcgagaatg cattgtggat ttatctgaac ggcgagaacc
     8221 ccgaaatgca ttaccgcctg acaatggaag ccagcccgtc gggggattac aaagtactga
     8281 ccatcgacag cggtgccgtc ggtcggctgt tctcctcggt cagtgacaag aaagaacttt
     8341 ccgaagtttt cagtactcca cactgagatt catgcgctct acgtggggaa cgcatgaagg
     8401 gtcggtgtgt ttgggtcctg gtgtcagagg cttgttcgac ttatgacagc tggcgccagg
     8461 ttatatttct gcgcacaata tcgccaagca gcgagtcatc gaccgtcagg ctagtctata
     8521 atccctctga agagttttta acgagccgag cgaaatcggc gaagtcgccg gccatttcat
     8581 catgtgcgca gatcagggac tgggccgatg ttgtagccat tctacggcaa tcgtcggcct
     8641 agtactccca ttgatcatca tctcgccgtc ttgtaacttt ctgaaaggtt aatgcctgaa
     8701 tctttgcaat gccctcagaa agcaagctat gcaatcacac gtcctacaaa cgcgctgaca
     8761 agtaatccgg cgcagcgagc tgccaaagtc cgctaacaat cgcgttccga tcatgtagaa
     8821 ggatctaatc caactgaaag gttctcatga actttccatc cgagcacgac tttggcctgc
     8881 tgatgtttca gctctttatc attttgaccg ccgcgctagg cctcggcgaa ttatttcgac
     8941 gcttcggtca agcgcaggcc atcggcgaag tcaccgctgg cattctgctc ggcccttcag
     9001 ttttgggcgc gcttttcccc ggacattacg aagctttgtt cccaccctct caaactcatc
     9061 ttttgggcac actggcttgg ctcggcagtg tttttctgct cctggttgcc ggcatcgaag
     9121 ttgatcttgc tgcgattcgc cgtgaaaaac gcgtgatctt ctcgacaagt ttcttcagca
     9181 tattggtgcc gttcgcattg gggtttagct ttgccgcgga acttccggac gcttatctga
     9241 gagatccttc ccaacggctt actttcgcgc ttttcgttgc cacggcccta tcgatctcgg
     9301 caatcccttt gattgcgaaa attctgatgg atttgaatct cctgaagaca ccgatgggac
     9361 aaaccattgt gggttgcgcg gtaattaacg atctcgtcgg atggatgttt ttcgcaatga
     9421 ttctgacaat gctcactgga aagatcggcg gtgaatcgtc cgtaatggca attgcattcc
     9481 tcactttggg tttcagttta gtgacgctga cggcgggaaa acacctggcc aagcgtctct
     9541 ttgcagcttt tcatcgccgc ggctttcctt acgagggaat tttgggattg gccgtcctgg
     9601 tggcattctt ttgcgccgcg gtgacgcagt ggatcggcat acacgctatc ttcggcgctt
     9661 ttctggccgg cgtgatgatt ggcgagaccg gcgagatcat caatcgcacc cgagagacat
     9721 tgcgcaatgt ttcgttctac gtcttctcgc ccgtgttctt cgcttcgatg ggcttacagg
     9781 ccaacttcat cgccgatttt gattggctcc tcgtcgctgg cacgattacc ctggcctgta
     9841 taggtaaagt aagcggcgcc acggtaggcg caatggtcgc cggcagaccg cgcagcgagg
     9901 cgctgaccat cggcttcggg cttttgccgc agggagccat ggggatgatt ctggcttttt
     9961 tggcattgca acacggcctg atcagtgatc cgatctttgt tgcgctgctg cttgcggcca
    10021 tcgccacttc cgtgcttgca ggaccgctca tccagacaat ggcgcccacg ctcaagcgga
    10081 gtctgagcac atcagaagaa cattaggttc cggtcaacac gcgacctcgc aaaaactttt
    10141 caccttaccg ggcgtcggct cttcagaagc gagcgctacg agggggcgaa gtcttttcat
    10201 gctccccgcg aaattgtgtc ggctactcca gattgtctgg ttcggattac gcgttcggat
    10261 ccagcgcaat cacatcgaga gcaaaatgcg cattgcgacc cggctgggcg ttggcatcgg
    10321 caacttgaat tgcgatacgc gccggcgggt ccttgggaaa gtattccatc caaacatcgt
    10381 ggaagagcag gcgatacttg agatcttgca gatagagcgt cacctttacg acatgctcca
    10441 ggccagcccc cgcggctgcc agcaagactt tcaaattctc aaaagcctgg cgcgcttgtt
    10501 ccttggtatc gtcactctcg taattggtta ggggatgatt accgcgaatc gcagaaaaaa
    10561 agatgaagcc accagctttg atcccttgga ccatcccttt tccatgggat ggtccgcctg
    10621 ccggggccat gacagtttct cgcttgatca tcgatcaacg ttctcctcgc gtcgaatttc
    10681 ctcgacttgt accacggctc tgtcaaacta ataaaggaca cacttcgttg cgatatggcg
    10741 aatgggcgtg gattgcacgg ggataaagta ggttggacaa ggcgccttac atggcaagcg
    10801 cgtcggagtt agtcgagcgc tcgacgcgaa agaccatcga aagaatctgc cagatacgat
    10861 ggagagcagg agatctggct tcgattcccc gctaaacgct caacctattg cgccggtttg
    10921 aagcggtccg ccggcactac tgcaaaggct tcgatctcaa ccatcagatc cggatgagcg
    10981 agtcgtttta cgccgatcag cgagccggtc ggatagtctg acgtgaaata ttcgtaacgc
    11041 acttcgttgg ctttacggaa ctcttcgaca tccgttgtaa agatcgttgt tttcacgatg
    11101 tcggagaact cgccgccgac tgaatgcaac gcacgtccga gattctcgca aatttgacgg
    11161 acttgggcgc gcatgtcgcc agcgtggacc ggcagcgcgt ctttctcgct tcgcgaggtc
    11221 atgccggcga tgtaaacgag cgttccgccg gtcaccgcga cgacttgaga atgtgttcct
    11281 ctttttggta ggtccttggg ctgaactatt attttattag cattggccat ctagctttcc
    11341 ctctcaacct tccgtcaggt taaagcgaca caaaaccaaa taggtttcgg cccgagtcag
    11401 agataaatac gtgcaccgat cttgtttgac agacaaacag gcttagcata agtcaaagat
    11461 acgtttccac atccaatcat aatccattcc atggccctgt tgaggagatt gataaagatg
    11521 aaagtgcttg ccgcgctggc gcttctactt gtctcaattg tttcaacgcg caactcggag
    11581 ttgaacgcag ctactgtaga ccaggttatt gcgagtgcca aaaaggaagg ctcactggag
    11641 ttctatgcgc ccggcacgtt gacagcgcaa ggtgcgaagc aactcgagga tgctttcaat
    11701 aaaatgttca gcagcgatat cagaattaac tattctcaat cgggcagcat gacggcggac
    11761 gtcagcaaat acatcggcga ggccgcggcg gggaacccgc cgcagtggga tgtcatggtg
    11821 ctcatcgacg cgcttcacgg atcaatttgg aaacgaaaac ttcatatccc tttcgactac
    11881 cgggtcctcg gagtgaacca aagggcgatc cactacaaca acggcaccgt gtctttcgcg
    11941 aatcaggtcg ttctaccagc ctataattct aagattgtag atcccaagga cgttcccagg
    12001 cgttgggaag atctcctcga tccgaagtgg aagggcaagc tcggcatggt taccggcgtg
    12061 gggcacatcg actatctcgc catgcgatgg ggcgagaaga agtttctcga cttcgggcga
    12121 gcattggcaa agcaaaatcc ggtgccggga aagttaggtg aactttatac tcggctccaa
    12181 attggtgaaa tctctttgac aactacactc acaaatagtt tcatcgtccg cgcgcaaaag
    12241 gaaggtgctc ccctggagca cgcacaagtg caaccggtgg ttgcgccggc ctaccatatc
    12301 ggcgtgctta aaggctcccg tcatccaaat accgcgcatc ttttcgcgtc ctttcttctc
    12361 agcctcgaag cccaagcgat ttgggagcgc tacaacggcc agacatcagc cttcgtgccc
    12421 ggaaccaaaa tgtataacta cgtgcaaggc aaagacgtga tttacctcgg ccaagacgat
    12481 gtcgatctgg tcaaccgagc ggtcgtcgag gtgaacaaaa tattggggtt caatccataa
    12541 ataggtcaag agaggaaaag tgcatgacaa atccgaaaag aatcgttcaa ccaaaagatt
    12601 taccgattcg caaatcacac tcacaggttg ttaccgtcac aggaggcaca ctggtgttta
    12661 tcgccggaat gacctcccgc agcgaagtcg atgccactcc tgtccaccgc ggcgatatgc
    12721 gagcccagct aagacagtgc tgcgagaaca tcggccgcgc cttacgcgcc gtcggcggag
    12781 actactcgga tgtggtgaaa acaacaacct ttacaaccga tgtggaagag tatcacaggg
    12841 tgaacgacga acgggcgaag tatttcaaat cggacctgcc gactagcgcg cttatcggag
    12901 tagtgcgtct agcgcaccca gacttgatgg tggaactcga agtgatggcg gtcatcccga
    12961 cggaacggtt taaccctcaa ggatgagggg cggggtggat gccatagatt tcgcttactc
    13021 cctctcttat atcaattgag cattgatagg gcgcaccgcc taagtgtcta aaacgaaggc
    13081 ttacacgttc atatcgacga gatctgtcgc gcagggggcg gtctcgagaa aatcccaact
    13141 cttcatccag tcgtaggtgc gttggagttc atcggccggg atcggcgccg gatcgaccag
    13201 ataaaggcgg ctctcgcgca aatcgtctac ggtgagctga ttgatctgcg gatcgcgatc
    13261 gcgatagtag tcgatgaaat agtgcatata agcgcgcttg tttgccatga tacgtcggac
    13321 ggcttcgcgt atcgcgcggt taaaggcggc gtaagtttcg gcatcgacgc gatcggacgc
    13381 tacgtccgtg ccgtggtgga aagcggagat gaccaagcga cagcccattt tttcagccag
    13441 tgaaatatac ggttcggtca gcgtggtcgc ttcgatggtc ttgtccatga gcatcttgaa
    13501 gcgataattg gagccgttcg gcacgcgtcc gatcttgata agctcacgcg gtacaaagcc
    13561 ttcgagcatc tgcaggcaga gatagtgagt gccgctataa aacgggacgc cgatctgcac
    13621 gtcggcgaat tgctgaggca cctgcaccgg cgaatcagcg cgcaccacga tggcggcaaa
    13681 attcaccatg gaacgacggc caacctgccg gctgccgacg ttggtggttt caacccgcga
    13741 gaaattgccc cattcgcaag cgttgtagag atcagcctgg cctgattcca tcatgcggcc
    13801 gtggctggtg tagcgtgaag cgtccttatg atcggtgatg tgcagctgcg ggcttttgtc
    13861 caggcccttt tcacgatcca tccactcgat ctggacgcct tccttttcaa acagaccctc
    13921 gtcataggcc acgagctcgg ccaatccctg aaacggcgca gtggtttcca atcgcagtgt
    13981 cttcatagat tcctctccct tgggcgttag gtaagctacc cctatacaat ggctggcgat
    14041 ctaacttctg tcaaacgcta tgcgcatttc ctggcgcatg attgaaacgc ttaacatatt
    14101 ccaatccggt gccgcgcgaa attggcaatc cccgccaccc aaagtaatcc taagcagtgc
    14161 cggattgccg atcatcaagc agttttgctc gccaagcatg ctcgcggtta ggcgcccatt
    14221 acccgttcct cgaaaacaat gcgctcgcaa agctgaatag aagtttgttg ttacaaaact
    14281 tctatttcgc gtaagtcctt cctagtcgaa aaggaccttt ttgtccggaa tattctctgt
    14341 catcactttc gtttccttag agacaacgtc tcttaccctg gcaccttgct tgctcaaagc
    14401 agttgcccgc cagaccatta ccagatcaag aacaaagagg tcactatggg agctgaagaa
    14461 accaaacacg atttatggct gaaaagaatc aatacctgga tcgcgatcct tggcggctca
    14521 gtcgcctcga tcgccgggac gtacaacttt ctcccctcct tttttccccc tgcccccggc
    14581 catatcgcgg ctgtggtccg cgaacaaggg ggcgctccgg ttccacgcgc gcatgttgag
    14641 ctgttaaatt ctgaaaatgt tttgctcgtt gcttctgaaa ccgatcgcga cgggcgcttc
    14701 agcagaaaag acctcgaagc cggtagttac cttgtaaagg taagcagggc tagcttcgag
    14761 ccgcagactg ccaaagtgag catcgcttcc aaaaagacca cggatttgga tctggtttta
    14821 aaatcgcggc cgcgcgctca agtctcgaac agtgcgccac cgaatactcc tcaaccgcgg
    14881 gctatttcgc agcctgaggg cagtgcgatt cgttcggcgc ttgaagaaac cggcgcagcc
    14941 tggatcaaga acctcagcaa tgccgggaga tgagctcaaa acgtcttcga actctagcag
    15001 tcgcctcata gattcgcagc agcgcattgg atcttagatt caatctaagg cactgctagc
    15061 cggtaaaacg tctgagtagt tagctccagt caccctctcg tccagtcact tagcgtcagc
    15121 actctttcgg ctgcatctgg cacgacgctt ctgcagacct cttaaacaca ggcgcaacat
    15181 tccgctcatg actgcggccc acacttcact ccgttagctt catctccgtc agcttcggca
    15241 cacggtcgac tgcgaccatt tacctcgttc gtgttagctg catcctcaac tcttgtatga
    15301 ctgctccgta aaagagctaa cagagtcaca tctgatgtga gcgcacgccg cagatggttc
    15361 ggaggcggaa tgatcaatct gcggcgctca cgcaaacagc cgcagcaacc acgggcgctg
    15421 ggaatcaatc atccattcag tatctggatt gtaggccgta gttcctgtaa cgatttgaac
    15481 taagcgcagc ggttgaacga tttgaaccgt tgcaacgaat tccttcaaag agtggaggtg
    15541 cgcaatgcgg gaagctcagc aaatcagtcg gcgaacgtgg ttggtccggt tcgccttggg
    15601 cagtttcgca ttatggaacg agctcaattt cggcttcggc cgcagaggtt ggggcatcgc
    15661 tattggaggt ggcgatctcg ctgttggcgt cgctcacgct cagaacctgg agccggcaaa
    15721 cgcgcttcgg gtcaacctgg gcattgtcaa tgcctacgtt cttatccgag gcaaggaggc
    15781 ggcggtggtc gataccggca cgcccaacaa tggatcgaag atcgcggatg tcgttcgcac
    15841 cgcgggcctg agttggaatg cggttcatca tgtgattctg acgcactatc atcccgatca
    15901 tatcggcagt gtcggagaaa tactggcggc ggccggaaaa gcgacggcat acgccggcgc
    15961 cggagacgtc gatcagatca agtcaccgcg ccctctcaag gcggtcaacg acaacgacga
    16021 agtgttcggg ctgcgcgtga tcgccacgcc cggtcacaca ccgggtcaca tatgcgtgtt
    16081 cgatccagcc ggctcgctgc tgatcctagg tgacgcgatg aacaacatcg gcaacaagct
    16141 cgctggtcct aatccccagt acagcgccga catggcccag gcgaatcaga gtgtgaagaa
    16201 gctggcgaag cttaagttcc agcgcgccgt cttcggccat ggggaaccga tcgacaacgg
    16261 cgcctcgcaa gcaatcacga aattggccgg caccctctga gccgtcgacc aagattcctg
    16321 ggatcagtcc gttgagctag cggtaccact cagaaactca gtgaaaacgg gccggatgcc
    16381 gaaggcgagt tagccgctac catgcggacg agaaagtcaa aagtaatcct gggtgaaaag
    16441 tcttcagccg agggtgcgat ccagaagcgc cggtcagtac tgcaacgcca tcaagaccaa
    16501 cccgtactta tcggccctga tagaggcgat cgatgaaacc gctttcatcc attcgcgtgc
    16561 aaagactcat gtcaacgaca tcgtcagtct gtaactggtc cgcttttgga ttaatcccgt
    16621 attgggccat gagtttaagg accaaagccg ttcctttaag agtaggacag ggcttccggg
    16681 gcagcgcttt caacatactt tgatacgcct cctggatcag ttcgggctta ttcaacttca
    16741 tatacttggc taatattccc tctacggttt tccggtttga ggggatgtga atgaaggcga
    16801 cggcttcagt aagcgccctc accatattct cggcgagttg aggattggat ttcatcagct
    16861 tgcgggacat gacgagtcct gttccctgaa acgggatact ttccttggcg gcatcgaaca
    16921 gaacattgaa gccttcccgc gcaatttctt gagacagctc cggtggaaga ctcgtcgcct
    16981 caacgctccc tgcgcgcagt gccgccatgc gtgcgggaac acttcctatt tgtgaaagaa
    17041 cgatattatc tcttttggga ttgagcccaa aatggtcgag gaccatgaag gtcatcagac
    17101 caggtaaacc cgagggcgtt ccaatggcca ctcttttacc cttcagtgct tcgacacttt
    17161 taatcgaggc ttgcgagaca aacaaataat ccaaggtatt aaagaagctt aaaaccattg
    17221 tcaggtcgcc acccgcgaca acagcattgg cgacgtgagt aatcgcgagc agtccgaacg
    17281 gaacctggcc ggcgagcata gcctgggttg cagttccggc aatgatgaaa accagatccg
    17341 ggtccaagcc gtactttcgg aaaaggcctt gttcctgggc aacccagagc ggggctaccg
    17401 cattgctaat cacaccgtaa gccacgctgg gtttagccgg cggctgtgca ccccaaaggg
    17461 atgggagtgg gggacctgcc agacataagc agaaagttaa aacgagaatt gcagagggct
    17521 gtaggaatcg cccgttttct tgcgtggacg ttttatcttc tctcttcatg gttccatgat
    17581 ctgcaaagct atgtagatct atttttgttt ccattatgtt ttgcagcgga accattaaac
    17641 aaggcttgca ccgggctaca acatggcaac gaaggtaggt ttccaagaat gtatttctcc
    17701 atatatgggg ccaccaaacg aagcctgaga tcatagaagc cttcgcactc acgcggctaa
    17761 acgaatagtc tttcccttat agtaggttcc ttgttgagca tccgtgtcgc tactggaaaa
    17821 gaagtcccgt aaataacgag agagtcgtta gagcggcggc gttattcatg gccggcttga
    17881 tcataggtgc accttgttgg ttcttccttt cgctgaattg aacggagagc aaaccgttgc
    17941 ttaacattat ggcgccatca agatgaggtc gtcgttaccg accttggtag aggcgctcaa
    18001 aaaacccgct ttcttctagt ttttggcaca ggctcatgtc gagaacatca tctggtacta
    18061 attgcgcggc ttttggatta atcccatact gtcccatgag tttaagaacc agagctgtcc
    18121 cttttgtact agggcaaggc ttccgcggaa gcgctgtcag aagagtttgg taggcttgct
    18181 ctaccaattc gggcctgttt agcttcaaat atttggcgag cgttacttcc acctgctttt
    18241 tgttcgacgg attatgaatg aatgctgctg cctcgatgag tgcctttgcc atattctcaa
    18301 cgaactgagg atgggattgc ttcagcttgc gggacataac gagaccggta ctctgatacg
    18361 gtacattttc tttggcggca tcgaacagaa cattgaaacc ttcccgcgca atttcttgag
    18421 acagctccgg tggaagactc gtagcctcaa cgctccctgc gcgcagtgcc gccatgcgtg
    18481 cgggaacact tcctatctgt gaaagaacga tgttatcccg cttgggattg aggccgtagc
    18541 ggtctaacac catatacgtc atcaagacgg ggacgccgga aggcgttcca attgcgactc
    18601 tcttgccctt gagttcctcg acactcttga tggaggatcg agcaacaaat aaatagtcta
    18661 acgtattcaa gaaccccaat accattgcca ggtctccgcc tccggaaacc gcattgacta
    18721 cattgttaat cgcgagcagt ccgaatggga cctggccggc gagcatggcc tgggttgcag
    18781 ttccggcaat gatgaaaacc agatccgggt ccaaggcgta ctttcggaaa aggccttgtt
    18841 cctgggcaac ccagagcggg gctaccgcat tgctaatcac accgtaagcc acgctgggtt
    18901 tagccggcgg ctgtgcagcc caaaccgatg tcagcgggcg agccgccagc cataagcaaa
    18961 aagagaagag gagaattacg gaagtttttt tgaattgttt gaattgttcg actgccatct
    19021 tatctcctct tcattcagga tcgacaagac cgtgagttac ctatttgttc ccgagataag
    19081 cctacggaaa actattaagc gaggcttgcg ccccctacag cctaattgcg gcgctttctc
    19141 tcttcggatg catcgatgcg cagccttcgc cgataaaagt aatctatttc ttgtcaagga
    19201 ggatgctcgg caaggttaca tgacatcgag cattaaggag gatgcgatcg gcctgattgg
    19261 ctccctgtaa ctcgcatgcg acccatgggt cggtgggagt tggggatttt tctgcaaccg
    19321 tacccgaata aacgatcaga tcgccgtcaa aggtagggaa agattggcgc accgtcaatc
    19381 tccgcaaaat gccgcgcaaa cctatccaat ctgttatggt acgaactagc aatgcctccc
    19441 gatatccgcc gcttgcgata aacgttgtta agccgctacg ttctcgcacg aaatctctat
    19501 cccagtgaag ccgataccag ttctcctgaa atccggccca ctgtaccgtg tcgacaatgg
    19561 tcaacggtcc aaaagcacgt ttcggaatag cgtagcccaa gtcgacgtct tcaaagcaga
    19621 gctgtttagt catttaaatc gaatcgttta aattactccg ctctccaata acgcctgctg
    19681 atcatatttc gagtagccaa gtcggttcat gatttctgcg gtatgttgac ccagtgacgg
    19741 tgcctgatgc agctcgctgt ggcgtgctgt cgatattgcc ccgccaggaa ccgtgatacg
    19801 gccgaattcg cggtgatcaa tatccctcag aaatccgcgt tccaagagct ggggatctcg
    19861 aaccagctcg accggtgtag cgaccggtga tgcagccatg tggcggctct gggctccagt
    19921 aaccagatat tctttggaga attgctttga ccaggcttcc agtcggtcga gaatgaaatc
    19981 tcttttttga tttcgtccgg tgtcatcgtc caacgcggga tccattgtga gcgccggatc
    20041 gccgacccat tccttgattt tcgtccaggc cgccggattg gggggaacac tgaccatcca
    20101 atgtccatcg gcacactcca gagccccaga gattgcagtt atctggcccc ttagcccgcg
    20161 ccggtgggtt atctcgcctg aggaggtaaa tgcgatcacg gcttgctcca tcaaggactc
    20221 tagcgactgt tgagcggaga cccggaccac gtccccctct ccgagcaagt cctgcatatt
    20281 catggctgct gcagtagcaa tcgcgacata caaaccgacg attacgtgtg cgacatgcgc
    20341 gcctgccaac acaggtctct cggatggctg tccagtgata tacatcaggc ccgaacgcgc
    20401 gtaagcacaa atctcgggta attcatcttc gagaaccgtc accacgaggc gtggattggt
    20461 ctggcgcagg actgtctcgt ccacgggtaa ggggttgttg gatactaaaa cgtcgcatat
    20521 gcgaacaaga tcaaggagaa ctttacaacc tgtctggttt ttcaggtcca gcgtgacact
    20581 cttttttccc atgtttaaaa acatgtggta gggaccatgc tccaaatcag gattctcggt
    20641 gacaagggga cctagccgcc ggagtctatc gcccgaacgc ggctctattc tgatgacctc
    20701 atgtcctgcc tcggcgagaa gtcgcgtgcc gtatgccgcg gaaacatccg tcaagtcgat
    20761 gatcgtagac acttctagga ctcaccttca ttgaccggag cattgcgctt cggaagtttc
    20821 acgcttgttt ggcgcaccaa gagaaccaaa ttccctttct cttgtctaaa aatcatctct
    20881 cgtgtgacaa aaatgccgac accggatcgc gtcctccggg ctactatatc aataatcctg
    20941 tgagtaacat taagtcgatc gccgatgtaa acgggctcga accattcgaa ctctgcttgc
    21001 gcgttgcgcg attcagtata gcctttggga agcggcacgg cttcccagag attgtcggag
    21061 tgcgtgccct ttgaaccctc gttcctacga tatagctcgg cgatcattgc gggagggacg
    21121 actcgacttc gatatccggc tgagcgcgcg atttcgtcat cttcccaaag agggtttcta
    21181 tcgccggtgg catttatgta gcggcgcaga tcggacgcag agatcggctc gggcaatggg
    21241 attggaccaa tcgttcgccc acaccatgtt ttgagttcat cgcttattaa aaaatttact
    21301 tcatctttca tgaatacctt attcgagagc accctggttt tctaattgtc gaatttcttc
    21361 atccgagagg ctgacccact ggcgcaggat atcccgattg tgctcaccga gccgcggcgc
    21421 gggcgtaggg ctcgccaaag agtagcggct gtaaatgaag ggaactttgg cgacgagtct
    21481 cggagcttga ggcggatgat ccatccgtcc gaatatactg cgctcatcac gcgattttac
    21541 gatctcgtca attcgcctta cccgctgagc cgggacgcca accgactgca gtaatcgttc
    21601 aacgtcggtg ttttgcagtt cacttgtcca ttgctccaaa tgcgcatcga tttcatcctg
    21661 atgctgcatt cgcccgagca ggttctgaaa cttagcatcc gaggcccaca atggattacc
    21721 gagcgctttt accagggcaa gccactgctc atcactttcc actgcaatca cgcaccattt
    21781 gtcttctccg gcgcaacgat aacaaccgtg cggtgcggcc gacattgacc gattgccgat
    21841 cctgggagga ttttggtgcg ttaacgacgc tgcaaaaagc agcgcgccaa cactcgcaac
    21901 gctgcattca aattggctaa cgtcgagata agttcccaag cccgattgat tgcgctccga
    21961 cattgcgtgc agtatgccaa agcaggcgtg taatccaccg acaaaatcgt tccaagaatt
    22021 tgagattgct accggggcct cgccatcgtt gcctgtcgca gtcatcagtc cagagtaggc
    22081 ttgaaggttg aggttcatgc tcctccagtt tcgtcgcggc ccactatgtc catagccgga
    22141 catactaacg aagatgagtc gaggattctg agtttgaaga taatggggtc ccaaccccag
    22201 cctttccatc actcccgcgc tgaaattctc caccagcaca tccgccacgg cggccatccg
    22261 agctgccagg tcgcgtccag cgtcggtctt caagttaatc gtgagcgatc gcttgttacg
    22321 gttgatgttt aaataggcag gcggacgttc atcaggctcc gaaccggcag gcttagttct
    22381 tgttccgccc gcgaaatcct ttcgcagact tgtctcaatc tttacgacat cggctccgag
    22441 atcagctaga atccgagtgc agaagggcgc tgcaacaata tggccgaaat cgaggactcg
    22501 aagcccctgc aacggttgcc tccggtttga accgttcatc actgtgtcag gatcttcttt
    22561 actcgctcca ccacatcagc cggttggttt atcgcttctt gtgccagacg ctctaattcc
    22621 tctccggata caaagtcgac atccattctt gccttttttg catccgccaa aagatctgga
    22681 tctttgagcg ctgcagcgta tccactacgc aagactttga tcagttccgc aggagtaccg
    22741 ggtggaacga caatcggctg accgaagtca ctacccagta atacgacacg aactactcgg
    22801 cggcttgcgt cggaagtttt gtacttgtcc ataatttcat agatagtcgg agcatcgggt
    22861 gatcgagaat ctcgcgtttt accggtctgt aaaatatgga gatcgaagtt attcttgtgc
    22921 cagctggtga atggttcgcg accgaaatgc gccgggaaaa tcattccacg acagtgcacc
    22981 tcaccttttt ctacggccaa atcgatctcc gttccgccgg cgtagcccag tacggtctgg
    23041 atttttactc ctaaagcctc ttgcagaacc ttcgctaaaa tataatccga gctggcaact
    23101 ccggagccac cgcatttagc cggctccttc gcctttaaca aatcttcaat ggtcttgtaa
    23161 ggcgcgtcag cccgcatgta gagaatcagc gggttagtga cgggcgagcc aatccagtgg
    23221 aatttccgaa tatcaaactg aacctccttt tgacttgaga gttgcgcaga ataaatgccg
    23281 gcgtgagggg cgccgagcgt gagtccgtcg ggcttagcaa cgttgtaaac gtagttggcg
    23341 gctattatag atcctgcacc aggcatgttc tgaacaacga aggtaggatt tcccgggatg
    23401 tatttcccca ggtatttcac caccaagcga ggccagagat catagaaacc accggcactc
    23461 agccccacaa caatccggat agtcttccct ttataatacg gctcctgttg agcatgaagc
    23521 gtcgtgggtg aaaagaagtt caataaaagt aataaccatg caatcgctaa agcggcagct
    23581 ttatttctgg gcggctttat catcgacgca ccttacttct ttctttgtgg cattgaggcg
    23641 agcgcaagag ttgctcagta tcgctagagg ttccccacaa gccgaaattt atagagtgtc
    23701 atgttttccg taacatcctc gaagaccacc tctacgggtg catcacaacg caactcctcc
    23761 agcgtgcacc cgacgatgtt acttacgaga cgcggacctt catctaattg caccaatgcc
    23821 actgcgtagg ggatctcggg ttcaaaccct ttgtgataga cacggtgata gactccgaat
    23881 gagtgaatct tgccctttcc gggcgagggt ttccattccc aatgcaggct caagcactgc
    23941 ggacatgtca cgctcgctgg aaaccagtac gagccgcact gcggacagtg gggcaagagc
    24001 aattgatgtt ttttgcatcc atgccaaaaa ggttcggact ccacggccgg tttaggcaac
    24061 ggctttctgt actcgctcat gacgcctccc tcgctagaat taccgcggac tggcatacgg
    24121 tattgccacc gtgacctgtc accagcgcaa tctgcgcatc cttcacttgg cgctcaggcc
    24181 catgttcatg gcgaatttgt cggacgccct cgcagatctg aagcatgcct tgcacatgcc
    24241 cttcggatag ttgaccgccg gaagtattgg tgggcagttc gccgtcgaac tccaaagcgc
    24301 ccgaagctgc aaaagggccg ccctcacctt ttttacaaaa gccatagtct tccagctgca
    24361 aaagaaccat gtgcgtgaag cagtcgtaca tctgtgcagt gtcgatatcc ttgggaccta
    24421 cgccagccat acggtatgcg gtctcaccgg cctccttagc gccagttgtg atcatattgt
    24481 cgtccgcgaa ccaacccttt aaacggttgc caaaaccgaa gccgagaacg tagaccggcg
    24541 gctttttcaa gtcccgggcg cgctccgccg acgtcaccac gaccgctcca gcggcatcga
    24601 cgtagacgct gcaatcgtac aacccaagcg gatccacaat cattcttgcc ttcatgtact
    24661 gctcgagtgt cagcgggtcg cgcataaagg cattggggtt tttgcgcgca tgattgcgga
    24721 aggcgaccgc gatcgagccc agctgttctt ttttggtgcc gaacaattgc atgtgacggc
    24781 gggcggcaat cgcatagtgc gccggttgac cgaacaggcc aaattcgggt ccgaattcct
    24841 tcaaggcctg ggcctcgctg ttggcggcca ccagcgcctt agcctcggcg ttgcgatgcg
    24901 tgcgcgacca ggcatctctg gcgtatccaa ccacgacggt gttgcacaaa ccggcatcga
    24961 tcgccatgca ggccatggcc acaccgagaa cttgactggc tccgccatta tccgtggagg
    25021 tgctaaatga tgcgtttatc cccagcgctt cgccaacgtg ttgatgatgg gtatacgaat
    25081 cgtcgggccc gtgggagatg agaccatcga cgtcggctcg cttcaatccc gcatcttcga
    25141 tcgcgttttt cgccgcgata gcaaaaagac cgatgtcacc gtactcggga atcttaccaa
    25201 gcggactttg accgacgccc acgatggcgt acttgcctct aaatttttca ctcatatggt
    25261 ttctccgaca ctccttccag gaagctttac tcagtctagc gcaccatcct gcttataggc
    25321 ggcaatggtc gtcgtgctgt agccgaccaa ctctcgcagc accgcctccg tatcccgccc
    25381 cagctcggga aactcccacc tgcgactcag cttcgccttg gcaaattgca gcggaaaatt
    25441 cggcagcacg acacgtccaa gtctcggatt ctccaccgca acgatgaacc cccgctcttt
    25501 aagatgagga tcatcgacta aatcccggcc cgtttgcacg acaccggcag gaacaccgga
    25561 ctgctgtaat cgattacgga ttgcgaaagc atcttcgtcc ctggtccatt gcgcaatgat
    25621 catgttcagg gtgtcgcggt ttttgagtcg gtcagcgctg gtcgtaaatc gcccgtcctg
    25681 ttccaggccg ccgcccacga ttcgcgctaa ggccgaccat tgttgatcgt catccgctgc
    25741 aatcacacac cagcgatctt caccggcgca tgggtagcaa tcatggggcg ccgatgacag
    25801 cgaagcattg cctgtcggct cagggttgct accgttcgcg accgcatcca tcaggttcgc
    25861 accgatcata aaagccgtcg cttccgcttg tgccaggtcg atgaaagcgc ccgttttgcg
    25921 gcgatcgcgg tgcaacactc cggaaacaat cagaatcgcc gacagcacac cggaaacata
    25981 atccgggagc accgtctgag agccgatagg gggcgcggtg ttgccgggat gattccacaa
    26041 ataagtaagg ccggtgaacg cagcaatgtt caccccaagc gtggcgaaat gccgttttgg
    26101 accggtagtg ccgaggcccg gcatgcgcag attgacgata tccggtttga tcttgcaaag
    26161 ctgctcgtag cccaagccga tgcgctcgac cacatcgacg ctgtaattgt ccatcacggc
    26221 gtcggctttt cttaccaagt catgcactat cttatgaccg ctcgggtgct tcagattgac
    26281 tgtgaatgaa cgcttattgc ggttgaattc gagaaagcgc ggcgacacgt tcggatcgcc
    26341 ggcctcgccg aaaaatcgaa atggatccaa accgcccttt ctcgattcaa ctttgatgac
    26401 gtccgcgccc agctcggcaa gaatcgttgt accatacgga ccggcgagca catgatcgaa
    26461 gctgacgatg cgcatgccat gcaacgggcc attgccgcga ggcacactag gattgcatgt
    26521 cggtgccggt gatcgcttga ttccatcatc ctgccagcta tcgagcaccg gtacgctgcc
    26581 gaccggcgga cgaacgccgt cgatgaggaa cggcattgcc gtttgttttg cgttgccgaa
    26641 acctgggtga tcgactgaga caataaatgc ccggctctgc acatgttgat cattgataaa
    26701 atccaatggt gtgtttaccg gtacacaggg aatgccttgg gactgtagaa gttcagtgat
    26761 ctcagtgacg cgatatttgg ataagaaggc ctcgacaccc gggttgagat cttcgacgtg
    26821 catacggcga tgaacgtaac tgagccattc ggggccatcg aaatgcggcg gatgatcttt
    26881 ccacaagtct aacaacaatt tccagtgctg acgcgtgacg tagagataca cgtagccatc
    26941 acgacagcga aaaatcttcg caggagctac cgatgcatgc tggctgccgg cacgcctaag
    27001 aacctccttt tcgtttgccc agagacggat aatatgctct tgagtcgcca ggcattcttg
    27061 catggaaacg tcgagatgat caccttctcc cgtccgctcg cgactgtaaa gcgctgctaa
    27121 gactcccacg gttgcgtata aactcgcgaa gtaataggcc tgggcttctg gcggtttgca
    27181 cggcggcaat tcaggcgttc ccgagatata aagaaaaccg ctctccgcca tcgcgaccag
    27241 atcgttgcac gcgagcttgc ttttcggccc ggtctggcca aacccggtaa tcgaagccat
    27301 gactatcccg ggatttgact tggtaagcct ttcgtatccc aacccgatcg ctgccaattc
    27361 gccggggtga aaactttcca gcaatacgtc ggccgattcg accaactcga gcaacgcgtc
    27421 gcgatctctc ttcttcttga gatcgaggat tctgctggct ttgccagcat tgagatgagc
    27481 aaaagtcgag ctcagccgcc ggccgtcggc agagccgatg aaaggtgcca attccctgac
    27541 cggatcccca ccgggaggtt ctatcttgat gacctccatg cccaaaccgg ctagcacgcg
    27601 cccgcacaac tgccccctca agtcggttag atcaagcgct cgatagccct ttagaaatgc
    27661 cccgcgggac tccatgttct cagcgacctc tcctgggact agcggccttt ccatacagga
    27721 ggtcgcttct cggcaaatgc gcgcgcccct tccttttgat cctccgtcgt cgaaagagcc
    27781 aaacccaatt cgctctcgag ctgcaacccc tggtcgatcg gcagctcgac gcctttggta
    27841 atcgcttcct ttatcgcgat cacagaaagc ggtgcgccaa ggcaaatttt cgtggcaata
    27901 tcctcggcaa cggccatcaa gcgatcaact ggaacaacac ggttcacaag gccgaggcga
    27961 aaagcctccg cggcattgac cagctcgccc gtaaggcaca actctagcgc gttcgcagtt
    28021 cctatcagac gtgggagccg ctgcgtccct cccgagccgc cgagtcgacc tcgacgaact
    28081 tccatcatac cgagcatggc atcctcggcc gcaacgcgca gatcgcacgc gagcgcgacc
    28141 tccaggcctc cgccgacagc gtaaccgcgt atcgccgcaa tcgtcggctt atccatcgaa
    28201 gaaatcgctt ggtgatgaaa atggatgcct ggctggcgtc gcccatgtcg ctgggcaaat
    28261 aggctttggt cgtcgctttg agccgctccc tcttttagat cgaagcccgt gctgaaagcg
    28321 cgctcgcctg atccggtgaa aatacaggcc cgcacttcag ggtcctttct catcctctcg
    28381 caggcatcga taatttcccg cctcatttgc tggttcaccg cgttcagaac ctgaggacga
    28441 ttcaacgtga cgaaaccgat atgatttctc acgtcgtaga caatggcttg gtaactcatg
    28501 atcgaccatc ctgattcaac gcccgctgca gttgaacgat cagcggattt gttttgacgg
    28561 attagacaga cgtgctcccc gcaaccgcta gggcctgaat ctgcttttat aatttcaaac
    28621 gtactcggca ctcttaacgg tgtggagcgc tcttgtcaat ttagaatttc caatgtagga
    28681 ggttagaaaa tttcttacgt gtgatgcctt gtggacttca agacattaaa tgcattttat
    28741 ctaaccatca gcaccgggag cacgctccga gccgccagct taatggagct gacggttccg
    28801 accctttccg tcaaactgaa aaatctggaa aaagatctca aagtaagact ctttgatcgc
    28861 ctccctaaca agcttgtgcc aacgccggag ggcatggcgt tttacggaga agtccgccaa
    28921 gtgctggaat gcattgagag agcgaaaacc gccgttcatc ggaagcaagg tcatagcttc
    28981 gagggccgta tttcaatctc catgggaagc gatctcattc agatcttcgc gcgccgattg
    29041 ggatcttttc tcaaacgaca tcctcgtttg gagtgcacgc tgctcgccag agcgtcttct
    29101 gagacgttct ctttggtgct ggctggcaac gtggatatcg gcatcggccg tttcgattcg
    29161 atgccgcgcg ccttgatcgc caaagatctt tttgaaaatt cgatctggtg cagttttccg
    29221 cgcaagcacc catttgcccg cgacggcaga atcagcgtcg gtgatttcga aagtcatcga
    29281 atcattacgc tcgctcggtc ctcaggaact ggacgcgcta ttgatcaagt gtttcggcgc
    29341 agccgagtcc ggcctcagaa catccttgaa gtcggaagct gtcggtcggc attggaattt
    29401 gtgaaactcg gagtcgggat cggcctggtg cacaacaatt gtctcattac cgagcgtaag
    29461 gataatcttc gcttcaaaga tgtgagccac atttttgggg ccgcgccagt cactttgatc
    29521 tacaaacgtt ccactattct aacgccagcc cacttgggga tcatggacat ttttgaccac
    29581 ggcccccgag gtcaccttta gagcacgtag gtcgcgcctc gtcagcactt gggcagaccc
    29641 gcatttatcg cagatttcgt tgcgctcgcg gcactacatc ggcgtgtcac tcgcgaaata
    29701 ttgcacgggt gggcgccaag caaagattta gcatgaactc ccggagcacg tcggaataca
    29761 agaagacttt aactttgtgc cttgtgaatt gcggcggcgc ggcttaagac cgatagtctt
    29821 atgccttgcg ctgacgcgat cgtagccccg tgaatcgtct gcagccagca aatgtccctt
    29881 gctacgccat tgacttgatt ctatagaagg accgatccag aaaggaacgt gtccatggca
    29941 caaaatagtg acgtgcgatt agccattctc tcccctggcg atcgtgcagc gaggcagaat
    30001 acaaggggcg agactagccg attcgcagat ttgttcaggg agtttgctgt cagggggatt
    30061 cacgtcgagg ccgcaatata tcacgatgat ttttgcgagg aagttcgaga gcagctcatg
    30121 cgggttgacg gcattctggt atgggtcaat ccgattgaag gcggacgaaa cagatcgatg
    30181 ctcgattcta tgcttcgtga tgttgcggcc gctggagttt ttgtcagcgc ccatcccgac
    30241 gtcatcttga agctgggtac gaaggaagtt gtttatcgca cgcgcaatct tggatggggc
    30301 tgcgacacgg atctatacgc cagtttcgat cagatggttc agaagctgcc tttgcggctg
    30361 gcaaccggta gggccagggt actcaagcag catcgcggca acggtgggaa cggcgtgtgg
    30421 aaagttcaat tggcaagaga cgaattagcc gatcgaaccg gcgccgcttc cgctgccttg
    30481 ccgcagccgg aaaccgtcat tcaagtacga catgcgttac gtggttgcag cgaagaggaa
    30541 atcaagctcg gcgattttta tcatcgctgc gcgccttact tttacgccaa tggcccgatg
    30601 attgatcaag aatatcaaga gcgactggcg gaaggcatga ttcgctgcta cctcgtgcat
    30661 gacaaggtag tgggttttgg ccatcaggct atcaatgcgt tgtttccagc gccccccgga
    30721 gcgccttcaa ccgaagcgcc cccgcctgga ccgcgtttat atcatccgcc gtcgatgccg
    30781 gaattccagc ccctcaaagt caagctggag caggagtggg tgccggcagc gcagcggctg
    30841 ctggacatcg agactgaaga tcttcctatt ctctgggatt gcgatttcct gctcggctcg
    30901 aagcgagaca gcggcgaaga ccgctatgta ctatgcgaaa tcaacgtcag cagcgttgcg
    30961 ccgtatcctg aatctgcggt gccttacata gtggacgcaa ccgtggctcg gttgcgggca
    31021 acgagacaaa gacgctgatg attgaagctc tcgttcaatc ccgtttgctg cgcatgccga
    31081 aaagcattga cgtggatctc gatcagcaag cgatatgatt cgctatgaat gtaggcatga
    31141 cattccagat gtagcatata ccggggtcag gcgagtctga cgaaacaacg tcgtgccgcc
    31201 cggccaaaat agttcgcggc aaaacagatc aataggagat tctgatagcc gtgtcgtccc
    31261 ctggcaacga acccaaagga cggccgtcta gagtcctcag cggacgggct gggcgcggtc
    31321 cacagccgtg ccgcgcagga tcaccgccga gatgcgtcgc gtattggtga tgtcatccag
    31381 cgggtttgcg tcgagcacga tgaagtcggc gctcttgccg gcttccagcg tgccggcgtc
    31441 ggtcagccgc agaaaatcgg ccgaattgcg ggtcgctgcc acgatgactt gcgccggcgt
    31501 cattccggcc atcaccatgt ccaacatctc ttcgtgcggg ccccacgggc gattgccgtc
    31561 ggttccgaga acgatgcgca cacccgccgc attcagcttc gccaggttgc gcgcctggat
    31621 tccgtagaga gcctgcacct taggccggtc tgtgttagcc gcttcgagct tcttgaactc
    31681 gtcggccgga aggctcgcgc gcagccagct caagtcggcc ttcactcccc gatccggcag
    31741 gttcggtgtg agaaccaggt taggacgctg cttgaacatg gtcaccagtt cgtcgtcgat
    31801 gtcccggtcg cgaacaccgt gcgcgaatgc gtcgatgccg gcacggatca gccccttcgc
    31861 gtccgcaagc tcgaagatat gcgcgatgac ctgcagcttt cgcttgtgcg cctcgtcgat
    31921 gatggcgcca tagatttcag gagtgagctt cttgtacttg ccgtctctgt catcgaccca
    31981 gatcttgacg atgtcgaccc tgtgcgccga gagttcctcc actgctttgc ggccttcagc
    32041 gacgctggta acccaataag gcactttggt tcgccccggc tccggcatcg taatgccgcg
    32101 cccggcgcta cgataacgcg cggcgccgtc aattgtctgg ctgtgcagat cgagcacttc
    32161 gtagccgtcg gtgcccagac tcagcaccgc gcttacgccg tagtaagcgc gcattctcag
    32221 gtcgcggatg actgcatcac gcgagtcact gagatgaaca tgagtgtcga ctatcatcgg
    32281 catcacggtc ttgccggcga gatccacgcg cttagcgccc gccggcacgc gaacgtcggc
    32341 agcgcggccg gcctgcgcga gtttggcgtc ctgcacgatc aatgttgcgt tctcaatcac
    32401 ccgcccgtcg ccgacgatca agcgcgcccg ttcgtatgcg gtgacgggct gctcggatac
    32461 cggcactgcg catccggcaa tcaaaccaaa cgtgatcact gcgagaaccg cgattaactg
    32521 cgctcgttgc atgaaagctc ctgattgcat tcttacgcga cctattaatt gaacggccat
    32581 ctatcttcta ccgaggttgg aattcgcgcc gaatcggccg caatatcgtt cgccgttcgc
    32641 aacctgtcaa gatgcaaacg cggccgaaca caacgaattc acgaagatcc ggcggagtat
    32701 ctcctgaaag catcgcgcat gcggctcaat ttagcgaata cattttgacg caaaatatgc
    32761 gctgaccttc aagtatgtgt cggcctatgt aatcttctcc cgtggaacgg atccgatgcg
    32821 gcgtctggag agtaatccgt catttcgagc ccgccaggag cctgccccaa ccttgtacgg
    32881 gcgagatgat gccggggcgc acgagatcgt tccatacttc agcgctcatg ttggtgaaag
    32941 ccggtttgcc atattcttcc tctatggccg gaatcacatg cagtgacatg gcggcaccgc
    33001 cggggatcga aatcgcttcg gcgtcaggcc aagttcttgc cgcttcgcgg ccgacatcca
    33061 gggccatttc caagccttgt tccaaagtca tggcgaagga gtccgacgcc cactgattcc
    33121 gcttggtgat gccgacgacg ttaatgccgc cagcctcgag gtagcgttta atggcctcat
    33181 tgaccgggtc ggcccagcgg ctgccgattg cgagtttcgt caatccgaga tggttcatcg
    33241 cggcaatcaa cgcctcgagc ggagcgtcgg ccgatatccc agttttttct ttcgtttcgc
    33301 gcagcaattc gcggaccttc gggcgggtca gcatcgccga aatcggagcg ccactaaaga
    33361 taatgtgatc gaccttctct ttgaccaacc ggtcgataca gatccagtaa ttctttatcg
    33421 ctttttcgac accctccttc gtataattct cgagcccgag ccccgtggag atttccatga
    33481 aatcgagcgg caggagccgg tacagttgat agcttcgtgc gcgctgcact tcttcatggg
    33541 cgcgtggctg gatcactccg tatcgaaaac gcggcagcag ttttttcaca tagtcggcca
    33601 taacgaacaa ctccttgaaa attgatgcaa ctggattgcc tcccgctcct gagcggactt
    33661 cctacttctc gtacaaccct ttgacgaagc cgcttgcctc gatttcgcgc gcgatcttgt
    33721 acgagacgaa ttgctccgga tcggccttct tcgccgccgg cgtggtgctc gagagctccg
    33781 tcagaatagt gcgaatccct tcgagtgtcg gaatcggcag cggctgatat ctgctcgcga
    33841 tctcgtcgta gttgtgctcc aaaacctcgc gatcgtcagt gcgggtatat cttctcagca
    33901 ccgccagcgc cgcttcttta ttgcgcttga acccatggat cgcttcgatg taggcgagca
    33961 tgaacccttt cacggtcgct tcattttttt gcagataaga tcgggtcgtg ccaatcgcgg
    34021 aggtcggata atccagaccc aggtcggcga gatcggccag ctcgcgaaaa cccgctttgc
    34081 gcgcgatcag ggatgaggtg ccggtcgtta ctccggcgtc gagcattttg gtctgcaggg
    34141 cgcggaccgt gtcctgcgat gtgccgattt ggagaatatt gacgtcgcgc tccgggttga
    34201 gtccccattt tttaagcgcc agccggaccg cgaaatcgga tgttgcccca tagcggctga
    34261 cccccaattt cttgcccttg agttgcgccg ggctggaaat ttccggagcg ctgaaaattt
    34321 tgaaaacaaa tccgttgatc agagaggcaa tgagaacggt gtcggcgccc tgcattgcgg
    34381 cttgcaccaa accggaaccc gagagcgagg aaatttgcag atctttggca atcagcgcac
    34441 gcaacgagac cgaacctgta cccatgccga ccagcaccac atcaagaccc tgcttctcga
    34501 acagtttggc ttccttggca aaccccaata cggcaacgcc gccatcggta gaaccatagc
    34561 cgatgacgac tttaacgagt ggcttggcgc ggtccgccag tgcgcttgac gtttgcggcc
    34621 aaagaagaga aatgacaagc gccgcgagca gtaatccatt aacaccctgc ggcagccagt
    34681 ggagatgcgc tcgaagacga gttttggtca caattttgcc tcgattggca cagccgagtt
    34741 ttccgtgctg aatataacgt tctccgccaa aacgtattcg ctgatctact ccctgtcaaa
    34801 ttcaactgat ctatgatcga tatctggaga atccaagggc acgcgttcgg tgaaacgcag
    34861 gataatcacg gcgaagatcc tgagagatct gtgagctact accccattga aactcgtagt
    34921 agcgctccac gcggtcgtcg ggcaattcac ctggttctca gctcgcgctg cgccattttg
    34981 agaatcgata gatccgcaac gtcgctgata gcgatttctc tgttgacctt tgccgtccgc
    35041 ttaacatcgt cgatcaaaag tctaagcccg tcctctggaa gagcgagatc ctcactgaat
    35101 gtctttacac tggaatcata ggtccgagcc gccatcgctt tatcgatctt caaccactgc
    35161 gccataatcg gcagggtgcc gtcgcgactc tgatgaatat agcggttggc tttgataccc
    35221 gctttgatga cccgcccgat ctcatccggg cgctctttaa ttttcttctc ggtagctatc
    35281 aaaccgctca ccggaaaact aaacaactca tgggcgcgcg ccagtactat gaagccaagc
    35341 gtctcaccga gaaaatcgat gggcggccct cctaacgtgg cgaaggtcag gccttgcttc
    35401 atcgcaccaa agcgggaatc gagagcgccg gtggcaagaa acttcacttc cttgtcggga
    35461 tcgatgccga aatgcttcat gattaatcgt cccattgctt cgagagcgcc gccgtaagca
    35521 ttgatcccta tggtttttcc cttcaagtcg gtgacagatt tcatctctgg ccgggcgatt
    35581 agcgcggtgg gagaaatggg tacgtaagca gcaaccagct tcactggaac tccacggatc
    35641 gccgcggcca ccccggggcc gagcaccgta taatagtcga tttcaccgcc gaccaacgct
    35701 tggagcgcga cggcaggatt catcctgata gactcagcaa cgatcccttc ttcttgaaaa
    35761 aagcctctct tatcgccgat agcgagcggc acaaacggtg cagcgagatc tgggaatccg
    35821 atccttattc tatccgccgc gtgaaccgag gggtggacaa gaaacccaaa cagcaagaga
    35881 aagacgtaag caaagcaacg tcgcatgaca gctcccttca cttgtctatt catctcggcg
    35941 cataggctcg aagaaacctc cgaacccagc aagcgtctta gcgatactaa cgaacccggt
    36001 ttattgaatt aggccatgac ttcaccgcca ctgtgaatcg gccggaatca ggttctttcc
    36061 gaagcggcgc tccggggccg cggctagcca ggcggaagtc ttctcaacgt aaagtttgaa
    36121 atgaggcgat tggcggtgcg cctccagcgc tgcctcatct ctatagacct catagtagta
    36181 aaagcggtta gcgtcatccc ggtcttgaat aacatcgaaa cgcaggcatc caggctcgtc
    36241 tcgctccgaa tgctccgcat cgtggcgcac cgcttccagg tattcgggca catgctccgg
    36301 tttcacctga accgtaacgt gcaatataac catagatcat ctccgtttct tcgatcgtca
    36361 ccgaatgaca gctcacacaa taccactcga tcctgcccaa gatgcaatga gaaaattaga
    36421 gcacctacgt cctcgcacgt tggcacagcc acctgctaat gacgaaccgg tgcgagcaca
    36481 tccccgagcg gccaaggcgc tcgcttgaaa ttcagcggaa tttcttggaa atcgaaatca
    36541 agctacagtg gctactcgac tcagacaatg catggattta tttcatgaac ctaccgagcg
    36601 tcgaaaatct tgtgactgcc agccatatcc ttgcagacca gggcgtgctc gacgggctcg
    36661 gccatatcag cgcgcgtcat ccggacaacc cgcagggtta tttgatgccg cgttctctcg
    36721 cacccgcatt tgtgacgctg cagacatcat cgaatacgac ctcgattcaa acgctatcga
    36781 tcgacaggac cgatcgcttt tcctcgaacg ttttattcat ggagaaacct acaaggttcg
    36841 gccggacgtt ttcgcagtga tccatagcca ctcgccgacg gtcattccgt tcagcatcag
    36901 gcagacccca ctacagcctg tattcaacgg cgccgcattt tctgctccat ccgctccggt
    36961 atttgacatt cgcaacatcg cgggcatgac cgacttgctg atcgggactg gcgagttggg
    37021 cagggcgctc agcgctgctc tcggagaaaa cgcagtcgtg cctcttgcgc ggccgcggca
    37081 acgtagtggt tgggaaaact ctgcagcagg ttgtttatcg aacgattcaa acacagctca
    37141 acgcccggct gcagcttcag gcgacgatgc t
//
LOCUS       DASUVS010000131         3444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1578816, whole genome shotgun sequence.
ACCESSION   DASUVS010000131 DASUVS010000000
VERSION     DASUVS010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578816"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(386..1339)
                     /locus_tag="VF182_07495"
     CDS             complement(386..1339)
                     /locus_tag="VF182_07495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6436959.1"
                     /translation="MLEIGYKLSSEEHQPQDLIAHAQRAEQCGFSFAMISDHFHPWID
                     AQGQSPFVWPVIGAIAQATSKLNLGTAVTCPTVRIHPGIIAQAAATVGSMMPGRFILG
                     LGAGENLNEHIFGHRWPPVRVRHAMLKEAVEIIRLLWTGKQENFSGEYYEVENARLYT
                     LPDTHPPIALAASGSNAAKLAGEIADGFVGTTPDAEVVDVFNRSGGSGKPRYGELTVC
                     WAENQSQAEETAYKLWPVSGLSGPLMSELPLPAYFEQAATMIDKTAVAKTVICGPDPE
                     LHWQAIQKYARAGYDHVFVHQVGRDQEGFFQFYERKILPRL"
     gene            complement(1500..1760)
                     /locus_tag="VF182_07500"
     CDS             complement(1500..1760)
                     /locus_tag="VF182_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX6436960.1"
                     /translation="MLGWIGWIIFGLIVGIVGKFLMPGRDPGGFIVTILLGIAGALLG
                     GFVGRALGFYQEGEPAGFIMAVIGAIILLAIYRAVVGRRRTV"
     gene            complement(1814..2368)
                     /locus_tag="VF182_07505"
     CDS             complement(1814..2368)
                     /locus_tag="VF182_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX6436961.1"
                     /translation="MADKPFLTDIKTLRDRARQHIEQGAVTPGYKADRDTVVKLLNEA
                     LATEIVCTLRYKRHYFMASGIHAQAVAQEFLEHANEEQGHADQISQRIVQLGGAPDLS
                     PESLTTRSHAEYVEGETLLDMIKEDLVAERIAIDSYREMAHYMGENDSTSRRLMEEIL
                     AVEEEHADDLVTLLQELGPEHGSR"
     gene            complement(2512..3138)
                     /locus_tag="VF182_07510"
     CDS             complement(2512..3138)
                     /locus_tag="VF182_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX6436962.1"
                     /translation="MPSATQMIRQDHRKVEDLFKKFEQTKGAQAKRRLAENAMAELEV
                     HAALEEEIFYPAVKKEVDDGSMVQEALEEHQTVKQLISDLKGMEEADEDFESQFSELV
                     ENVQHHVQEEESEMLPKAEESELDLNSLGEQMAQRKQQLQSGGRASKKPSNATSKRKS
                     AGSKSTGRKSAGKKSASSKTAARKTSTAKKNTGRKSTKKSGSRSRRRA"
     gene            complement(3175..>3444)
                     /locus_tag="VF182_07515"
     CDS             complement(3175..>3444)
                     /locus_tag="VF182_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6436963.1"
                     /translation="ERTFSSRDQFAPELIYFSNCIIHHLDPEPSGAEGLADVRVIRAL
                     YRSAESGRAVQLEPYIKRQRPGLEQEITRPAVEKPELVNVQGASQ"
BASE COUNT          707 a          985 c          908 g          844 t
ORIGIN      
        1 tatgccaggc gtttcgacga aattgcgttg cattctcaac ggcgcctttg gggaaggcgg
       61 aatgcgagcg cggattctgt cccaaacgcg aataaagccg atgggagcgt cttccgcaac
      121 tgggacgagt aaactagata aactagtcta cattgacaat gagacgaaga gcgcgatggg
      181 tccttgcctg cgtaatcgtg acttgctggt aatgatcaga ttgtgaggaa atcggcaggc
      241 gccgaggcat aatcggtgga tcggtgcgtt tactgaaggg aaatagccgt caccctctcg
      301 gcgatttagt gagcagtagc gaataaggaa cttggccgaa caatccagct aggaacgcat
      361 atcgcatccg agtgagcgca acagatcaga gccgcggcag aatctttcgc tcgtaaaact
      421 gaaaaaaacc ttcctgatcg cgacccactt gatggacaaa cacgtggtcg taacccgcgc
      481 gcgcatactt ctgaatggct tgccaatgaa gttcgggatc gggcccgcag atcacggttt
      541 ttgccactgc agttttatcg atcatagtgg ctgcttgttc gaaatacgcc ggcaacggca
      601 actcggacat cagtggcccg ctcagccccg agaccggcca cagcttgtaa gccgtctcct
      661 ccgcttgact ttgattctcc gcccagcaga cggtgagctc gccgtagcgt ggctttccgc
      721 tgccgccgga ccggttgaaa acatcgacca cctctgcgtc gggcgtcgtg ccgacaaaac
      781 cgtcggcgat ctctcctgcc agcttggcag cgttggagcc gctggcagcg agcgcgatcg
      841 gagggtgcgt atcgggcaac gtataaaggc gcgcgttctc tacctcgtaa tactcgcctg
      901 agaaattttc ctgcttaccg gtccaaagca ggcggatgat ttccacggct tccttgagca
      961 ttgcatgacg aacgcgcacc ggcggccagc ggtggccaaa gatatgttcg ttgagatttt
     1021 cccctgctcc gaggccgagg atgaagcggc ccggcatcat ggaaccgacc gtcgccgccg
     1081 cttgcgcaat gatgccggga tgaatcctga ccgtcggaca agtcactgcg gtgccaaggt
     1141 tcagcttgct agtcgcttga gcgatggcgc cgatcaccgg ccatacaaac ggactttgtc
     1201 cctgcgcatc gatccacgga tgaaagtgat ccgagatcat cgcgaagctg aatccgcatt
     1261 gctctgctcg ttgggcgtga gcgattaggt cctgtggctg atgctcttcg ctggagagct
     1321 tatatccaat ttcaagcacg cacacttctc ccttgccccg ccggcccgag ttctgagagg
     1381 cctgattggg aaaggtcaac tagcttcaac gttagaagtc caaggttcaa actccagctc
     1441 attggagcgt caaggttgaa ccttgaacct gaacgtcgaa tcgaagcggt aagagctcgc
     1501 tagaccgttc gtcggcgacc gaccaccgcg cggtaaatcg ccagcaaaat gatcgcgccg
     1561 atcactgcca taatgaagcc ggccggctcg ccttcttgat aaaagccgag agctcgcccg
     1621 acaaagccgc ccagcaaggc gccggcgata cccagtagaa tcgtgacgat aaatcctccg
     1681 ggatcgcgcc cgggcattaa aaacttgccg acgataccga caatcagtcc gaagataatc
     1741 cagccaatcc atccaagcat agtcgtttcc tttcttccct gaagttaatc tagccgattg
     1801 aacagttcag tgtttatctc gagccgtgtt ccggacccaa ctcctgtaaa agcgtaacca
     1861 gatcgtcggc gtgctcctct tccaccgcca ggatctcttc catcaagcga cggctggtag
     1921 agtcattctc ccccatgtag tgggccatct cgcgatagct gtcgatggcg atgcgctcgg
     1981 ctaccagatc ctctttgatc atatcgagaa gcgtttcgcc ttcgacatac tccgcatgac
     2041 tgcgcgtggt caagctctcc ggtgacaaat cgggcgcacc accgagctga acgatccgct
     2101 gggatatttg atcggcgtgt ccctgctcct cgttcgcatg ctcaagaaac tcctgggcca
     2161 ccgcctgagc atggattccc gaagccatga agtaatgccg tttgtaacgc agcgtgcata
     2221 caatctcggt cgctagcgct tcattgagta gcttaacgac ggtgtcgcga tcggctttgt
     2281 agcccggtgt gacagcgcct tgttctatat gctgccgggc ccggtctcta agcgttttaa
     2341 tatcggttaa aaaaggctta tcagccataa accactcctc tcaggtaaac ttactaacgc
     2401 ttctatcgat ctggcggtta taacgcaaac gggatgatgt tcgcttcgcc gtctgttcaa
     2461 gctgcactag ccagagttcg cacccttgat ccggcgcaag tttcctgttg cttaggctcg
     2521 ccgcctggac cggctgcctg attttttcgt cgacttgcgt ccggtgttct tcttggcggt
     2581 gctggtctta cgcgccgcgg tcttgctgct tgccgacttt ttgcccgcag attttcgtcc
     2641 ggtagacttc gatccggcgc ttttacgttt cgacgtggca ttcgatggct ttttgcttgc
     2701 gcgaccgccg ctctgcagtt gttgcttccg ttgggccatc tgctcgccca gcgagttaag
     2761 atccaactcg ctctcttctg ccttgggcag catctcactc tcttcttcct gaacgtggtg
     2821 ctgcacattc tctacgagct cggagaattg cgattcgaaa tcctcgtctg cttcttccat
     2881 cccttttaga tcgctgatta gttgtttgac ggtttggtgt tcctcgagcg cctcctgcac
     2941 catggaaccg tcatcgactt cctttttcac cgcaggatag aaaatttcct cctccagcgc
     3001 agcgtgaacc tcaagttcgg ccatggcatt ctcggccagg cggcgtttgg cttgcgcccc
     3061 cttggtctgt tcgaattttt tgaatagatc ttccactttc ctatggtcct gacgaatcat
     3121 ctgagttgca ctaggcatat cgggttcctc catcgttagt atatttggtt gtttttactg
     3181 cgacgcgccc tgtacgttta cgagttcggg tttttccaca gcgggccggg ttatttcctg
     3241 ttccaatccc ggtcgttgcc gtttgatata cggttccagt tgcaccgctc tgccgctttc
     3301 ggccgaacga tataacgcgc ggatgacacg cacgtcggcc aatccttccg ctccagaggg
     3361 ttccggatcg agatgatgga tgatgcagtt ggaaaaatag ataagctccg gagcaaattg
     3421 atcacggctc gagaaagtcc gttc
//
LOCUS       DASUVS010000132         4824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1578956, whole genome shotgun sequence.
ACCESSION   DASUVS010000132 DASUVS010000000
VERSION     DASUVS010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578956"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VF182_07520"
     CDS             complement(<1..476)
                     /locus_tag="VF182_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6436964.1"
                     /translation="MTYTMISADDHIDLGYLPRDLWTARLPESLRERGPHVEDRGEKG
                     EYWVCDGETWAEYRGERWFAKSNRTRLALDRGGVGEAHRPTTPEKRLADMDRDGVEAS
                     LMFPPIIAMQVGEPELRNACVRAYNDWALEFGKAAPTRFFPVAMLSPVDAQDAADEV"
     gene            complement(561..1184)
                     /locus_tag="VF182_07525"
     CDS             complement(561..1184)
                     /locus_tag="VF182_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_259446976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1140..1142),aa:Sec)
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEX6436965.1"
                     /translation="MPDPLVLEVFSDFVUPWCYLVTGRVEKLKEKYGLTIKFTQFPLH
                     PETPEEGNPRGPEIIARNRKMKVNMDREGLPFNPDRDMSYNSRLAQELAKWAGTKGIE
                     EKITAALFRAYFVDVKNIGKADVLAEIAAENGLPADEATDILLSRAYKDAVDEDWRRC
                     AAYGVNAVPTFLAGRYLMVGAQPYEELERLIQHVMNPQENVEAVDPR"
     gene            1718..2104
                     /gene="pcaC"
                     /locus_tag="VF182_07530"
     CDS             1718..2104
                     /gene="pcaC"
                     /locus_tag="VF182_07530"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HEX6436966.1"
                     /translation="MSDDRFEKGMALRKSVLGAEHVDRAEAGKTEFDADYQRYITENA
                     WGTIWTRPGLDKKTRHMLTIAMLAALGKHDELAMHIRATGNTGVTPEELREVLLHVSV
                     YAGVPAANSAFAIAKRVYQEIEEGKK"
     gene            2123..2839
                     /gene="pcaH"
                     /locus_tag="VF182_07535"
     CDS             2123..2839
                     /gene="pcaH"
                     /locus_tag="VF182_07535"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEX6436967.1"
                     /translation="MKIQPYKQRDWKSQPPYIFAGYGSSVKRGPLQKLVPINQTLSEI
                     TGPLFSGIKLESGENDLTRHPETGQEAVGERIIVVGRVIDDDERPIANSLIEIWQANA
                     AGRYRHPVDQHNAPLDPNFIGAGRCITNAKGEYRFLTIKPGAYPWLNHANAWRPAHIH
                     LSMFGPSFVTRLVTQFFFPGDPLLEMDPILSSIPTKAGRQRLIAAYAHDVTEPEFALG
                     YRFDIVLRGRQATPFEQNGR"
     gene            3081..3647
                     /gene="pcaG"
                     /locus_tag="VF182_07540"
     CDS             3081..3647
                     /gene="pcaG"
                     /locus_tag="VF182_07540"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEX6436968.1"
                     /translation="MKQTPSQTVGPFYSIGLTDRAMNTLVGESTQGQRIRIEGQVIDG
                     DGKPVPDAMIEIWQANAAGRYNHPDDKQEKPFDPSFTGWGRSGSEPSGSYFFETIKPG
                     PVPGSDESVQAPHINMIVFARGMLVHAFTRIYFSDEPANETDPVLNSIEKERRQTLIA
                     NRREESGKVVYRFDIRLQGENETVFFDM"
     gene            3667..>4824
                     /gene="pcaB"
                     /locus_tag="VF182_07545"
     CDS             3667..>4824
                     /gene="pcaB"
                     /locus_tag="VF182_07545"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HEX6436969.1"
                     /translation="MRVLDSAIFSPLFSDAEISALLTDEAFVRALVEVEGALARAQAR
                     VGVIPAAAAEQLSRVKAADIDITALTKGTIRSGFPIIALVQQIRRQAGEEAAPYVHWG
                     ATTQDIMDIASVLQMRAAIELFKPTILNIIRRLSELAQRHRTTVMAGRTHGQQALPIT
                     FGLKVASWIAPMLRHVERLDEIFPRLLVIQFGGAAGTLAALGDKGLAVSEALADELNL
                     SVPVMSWHAQRDSLVEFGGWLSLVTGCLGKMAQDVILLAQTEVGEAAESGEQGRGGSS
                     TMPQKSNPITSELILAAARTNASLLSALHHAQIQEHERATHGWQVEWLTLPQMMIITG
                     GALKHALDLAQNLQVDEAAMRVNMVRANDVVLAEAAVFALANWMPRAKAEEL"
BASE COUNT         1066 a         1330 c         1402 g         1026 t
ORIGIN      
        1 acttcatccg cggcgtcttg agcatcgaca ggggagagca tggctaccgg gaagaatcgc
       61 gtgggcgcgg ctttgccgaa ttccaacgcc caatcgttat atgctcggac acaggcattt
      121 ctcagctcgg gttcgccgac ctgcatcgcg atgatcggtg gaaacatgag cgaagcttcg
      181 acgccgtcgc gatccatatc ggcgagccgt ttctctggag tggtcggccg gtgcgcctcg
      241 ccgacgccgc cgcggtccag agccagcctg gtccggttgg atttggcaaa ccagcgctcg
      301 ccacgatact cggcccaggt ctcgccgtcg caaacccagt attccccctt ttcaccccga
      361 tcttccacgt gggggccgcg ctcgcgcaaa gactcgggca ggcgcgccgt ccaaaggtct
      421 cgaggcaagt agcctaaatc gatatggtcg tcggctgaaa tcattgtgta cgtcataagc
      481 ttctcctcgg accagggcag gcacagaggc ctgcccctac attaaaatga atcgccgtta
      541 ggaactccgc aacggttcct ctatcgagga tctaccgctt ctacattttc ctgcgggttc
      601 atcacgtgct gaatcaatcg ctccagttct tcatagggct gcgcgcctac catcaaatag
      661 cggccggcaa gaaatgtcgg cacggcattg acaccgtagg cggcgcaccg gcgccagtcc
      721 tcatccacgg catctttgta agcgcggctt aacaaaatat cggtagcttc atccgccggc
      781 aagccgtttt ccgccgcgat ctcagcgaga acgtcggctt tgccgatatt cttgacgtcg
      841 acgaagtagg ctcggaacag agcggcggta atcttttctt cgatgccttt ggtcccggcc
      901 catttcgcca gctcctgagc caaacggctg ttgtacgaca tgtcacgatc aggattgaac
      961 ggcagccctt cgcgatccat gttcactttc atcttgcggt ttcgcgcgat gatctccggc
     1021 ccgcgtgggt tgccttcctc aggagtctcg ggatgaagcg gaaactgagt gaacttgatc
     1081 gtcaatccat acttctcttt gagtttctcg acacgcccgg tgacgagata gcaccagggt
     1141 cagacaaagt cggagaaaac ttcgagaaca agcggatcag gcataggacc tcctcctaag
     1201 attttgttca accgttcaac tagaactgct tcaaaaaagt tcaaagcgtt caaacagttc
     1261 aagcagtttc gaacgctgac aaagttccaa aacgttgttc gttgttcacg agcggcttcc
     1321 gatcgttgaa ctgttgagcg ttggaactct tacactcttt gaaattgatt ctatgtttgt
     1381 tggtccgtgg aagtcaaagc ttcaatgcga aaaatgcagg gcaagttgac aatacttcac
     1441 atgcagggat agtttccaga gctgtatggc ggtacggtac tcttgaaccc gcaatgctga
     1501 cgagcgcagt tttcaggatc aagttcagcg cgcggttttg atgaaagagc ttgcctacta
     1561 cggcaccttc ttcagcgaat cgctcatgag cgggctcgat gggttccaag tagttgcggc
     1621 tgcggcaacg agcaatttgc gcaatgcaac agacaggttt gggtctactc agcatcgaaa
     1681 acgcgcaaac tgacggaatg tttgcaggag gtgcacgttg tcagacgatc gctttgaaaa
     1741 gggcatggcg ctgcgcaagt cggttttggg cgccgagcac gttgaccgtg ccgaggccgg
     1801 taagactgag ttcgatgccg actaccagcg ctatattacc gagaacgcct ggggtacgat
     1861 ctggacgcgg cccggactgg ataagaagac gcgccacatg ctgacgatcg cgatgctcgc
     1921 cgccctgggg aagcacgatg aattggcaat gcatatccgc gccacgggca acaccggcgt
     1981 gacgccggaa gagttaaggg aagtactgct gcacgtctcc gtctatgccg gggtgccggc
     2041 ggcaaacagc gcatttgcta ttgccaaacg agtttaccag gaaatcgaag aaggcaagaa
     2101 atagcaggtc ggagtttgag aaatgaaaat ccaaccctac aaacaacgtg actggaaatc
     2161 gcagccgcct tacatcttcg cagggtacgg ctcgtcggtg aaacgcgggc cattgcaaaa
     2221 gctcgtgccg atcaaccaga ctctgtcgga gattaccggg ccgctgttta gcggcatcaa
     2281 actcgagagc ggtgagaatg atctcacacg ccacccggag accgggcagg aggccgtggg
     2341 cgaacgcatc atcgtcgtcg gtcgggtgat cgacgacgac gagcggccga tagcgaattc
     2401 gctgatcgag atctggcaag ccaatgccgc cggtcgctac cgccatccgg tggatcagca
     2461 caacgcgccg ctggatccga actttatcgg cgccggacgg tgcattacga atgcaaaagg
     2521 agaatatcgc tttttgacca tcaagccggg cgcctatccg tggctcaatc acgccaatgc
     2581 ctggcgtccg gcgcatattc atctatcgat gttcgggcct agtttcgtca cccgtttagt
     2641 tacgcaattc ttttttccag gcgatcctct tttggaaatg gatccgattc taagttcgat
     2701 tcccaccaag gcgggccggc agcggctgat tgcggcttat gctcacgatg ttacggagcc
     2761 ggaatttgcg ttgggatatc ggttcgacat cgttttgcgc ggccgtcaag ccactccttt
     2821 cgagcagaac gggcgatgaa cccgtatatc gcagaagagg tcgactgtgt actgagcgag
     2881 cggcaagccc ggaggagcga aggcacagaa gcgaacactt tcaatgcatt tgatggtacc
     2941 tggtttaggg atagtagttt ttccgggggc ccgctcgagc aaccgaagat tgcaggctgc
     3001 tacaagccac tggcccattg cctttgctcc ggagggcttg ccgctcgatc gctgtgtcat
     3061 tttgaactgc ggaatcgtcg atgaagcaaa caccgtccca gacagtcggg ccgttttact
     3121 ccattggcct caccgatcga gcgatgaaca cgctagttgg cgagagtact cagggccagc
     3181 gcattcgtat cgaaggacag gtcatcgacg gagatggcaa gccggtgccg gacgcaatga
     3241 tcgagatctg gcaggccaat gccgcgggcc gctacaatca tccggacgat aaacaggaga
     3301 aacccttcga tccgagcttc accggctggg gccgttcggg aagcgagccg agtgggtctt
     3361 attttttcga gacgatcaag ccggggccgg tgcccggttc ggacgaatcc gtccaagcgc
     3421 cgcacatcaa catgatcgta tttgcccggg gcatgctggt gcacgccttc accagaattt
     3481 acttcagcga cgaaccagcc aacgaaaccg atccggtgct aaattcgatc gaaaaagagc
     3541 gccggcagac tttgatcgca aaccgccgag aagaaagcgg caaagtcgtt taccgtttcg
     3601 acattcgtct tcagggtgag aatgaaacgg ttttcttcga tatgtgacgt ccgctagctt
     3661 cgaactatgc gtgtgctcga ctcagcgatt ttcagcccgt tgttttcaga tgcggaaatc
     3721 tccgctctgc tcacggatga ggcctttgtc agggcgctgg tcgaagtcga aggcgcgctt
     3781 gctcgcgcac aagccagggt cggggttatt cctgccgctg cggcggagca gctatcgaga
     3841 gtaaaagccg ccgacatcga tatcactgcc ctgacaaagg gaacgattcg ctccgggttt
     3901 ccgatcatcg ccttggtgca gcaaatccgc cggcaggcgg gtgaggaagc ggcgccctac
     3961 gtgcactggg gcgcgaccac ccaggacatc atggatattg cctctgtgct gcagatgcgc
     4021 gccgctatcg aacttttcaa accgacaatt ctcaatataa ttcgacgtct gagcgaactg
     4081 gcgcagcggc atcgtactac cgtgatggct gggcgtaccc atgggcagca ggcgctgccg
     4141 atcactttcg gcctgaaagt cgccagctgg attgcgccaa tgcttcgtca tgttgaacgc
     4201 ctcgacgaga tctttccgcg cctgcttgtt atccaattcg gtggcgcggc aggcacctta
     4261 gcggctctgg gggacaaagg tctggccgtt agcgaagcat tggcagacga gctcaatcta
     4321 tccgtgccgg taatgtcgtg gcatgctcag cgcgacagct tggtagaatt cggtggttgg
     4381 ctcagcctgg tgaccgggtg cctcgggaaa atggcgcagg atgttatctt gctggcccaa
     4441 accgaagttg gcgaagcggc cgaatcaggc gagcagggca ggggtgggag cagtacgatg
     4501 ccgcaaaaaa gcaatccgat caccagcgag ctcatactcg cggcggcgcg caccaatgct
     4561 tccttgctat ccgcgctgca tcacgcgcaa atccaagagc acgaacgggc gactcacggc
     4621 tggcaagtcg aatggctgac tttgccgcaa atgatgatca ttaccggagg cgcgctcaag
     4681 catgccctcg atctagcgca gaatctgcaa gtggatgagg cggcgatgcg ggttaacatg
     4741 gtccgggcta acgatgttgt ccttgccgag gcggcggtat tcgcgttggc caactggatg
     4801 ccgcgggcta aggcggagga gttg
//
LOCUS       DASUVS010000133        35209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1360781, whole genome shotgun sequence.
ACCESSION   DASUVS010000133 DASUVS010000000
VERSION     DASUVS010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 35209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35209
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360781"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VF182_07550"
     CDS             complement(<1..588)
                     /locus_tag="VF182_07550"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6436970.1"
                     /translation="MKRYFLNRLLLLLPTLFGALTLVFFLINLVPGDPVEVMLGETAS
                     ATDKQELRRQLGLDQPVLTRYTSFLADLWRGDLGHSLYENVEVAELIAARVPATIQLT
                     ISAMIVAIVIAFPLATLAALRRDTWIDRVVLFFSLLGLSIPNFWLGPLLMILVSIQLG
                     WLPVSGRGDFSHLILPSLTLGMAMAAILTRILRASL"
     gene            complement(585..2045)
                     /locus_tag="VF182_07555"
     CDS             complement(585..2045)
                     /locus_tag="VF182_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436971.1"
                     /translation="MIGIESSPSQLDPRYATDANSVRISALIYSSLLRLDEKSRLVGD
                     LAERWTMIDAQTYSFRIRRGVTFHDGRALTARDVKYTYDSIMDRRNRSPKLGSLRVLE
                     SVEQLGSDEIRFHLSEPHAPFLEHCTIGIVPVDSRVPRENGRQQAPPGSGPFALAEIN
                     PGEKVSLKANASYWEGQPRLAGLVFQIVPDAIVRVLEFQKGTIQLLQNDIEPDTLPWL
                     EKNTDAVVETNPGTTFQYIGINLTHPILRNRDVRQALAHSIDRDGIIRHILKDQATAA
                     NGLLSPLNWAHDDSVPRWRFDPHRAQQLLDQAGYPDPDGEGPRPRFKLSFKTTNIDLR
                     RRIAETLKEQLSRVGIELEVRTYEWGTFYSDIKKGNFHLYSLAWVGIVDPDVYYNIFH
                     SSSVPPNGDNRGRYGNPVVDHLLEQGRRESDRRQRKAQYHQVQQIINQDLPYIPLWWV
                     KNIVVRKPQVVGFTPYPDGDLFALRRTSLSHSPHLP"
     gene            2400..3230
                     /gene="ccsA"
                     /locus_tag="VF182_07560"
     CDS             2400..3230
                     /gene="ccsA"
                     /locus_tag="VF182_07560"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX6436972.1"
                     /translation="MTVGMSLILLKLTAILYLIATGSFVFYVVRRDTASRLPPLILGA
                     GFIVHTLALSAYFMREGFPALTEMREALLFKSWLMVACYLLIQIKYRLTVLGGIIAPL
                     AFLMSLAGIAFGSGSGEIPPDLNTFWLPLHVTSAFLGNAVFALAFSVSLIYLLQERHL
                     KHKRMTALMKRFPSLESLDRLNYVLLGWGFPFMTLGIITGSIWAGIYWGNYWSWEPRQ
                     ISSGIAWLFYGALLHGRITAGLRGKKAAFLTIVGFTVVLGYFLWGDAIFPGRHGGRFE
                     "
     gene            3234..4514
                     /gene="hemA"
                     /locus_tag="VF182_07565"
     CDS             3234..4514
                     /gene="hemA"
                     /locus_tag="VF182_07565"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX6436973.1"
                     /translation="MHTEIFIVGLNHRSAPIEVRESVAFEKSQVTEALAQLRKISEAR
                     ETVILTTCNRVEVVAAAPDTDHAMEGVKRFLDQQRSPSHNGSLSDHLYSYRGADAVRH
                     LFRVAASLDSMVVGEPQILGQLKEHYNAAQKAGTVGAVLHRLFHRSFFVAKRVRTETA
                     IASRAVSVSSVAVELAGRIFDRFDEKTVMLIGAGKMGDLMARHLQRCGVKSIMVTNRT
                     FERAVELAEKIHGNPIRYDDFPRYLKLADLVIGCAGAPETLVDAGMVDKVLRERKQAP
                     MFFIDIGDRRNFDPGINAIDNAYIYNIDDLKSVAEENLNERSSEAEKAESIVQEEVGS
                     FLDWVSSLEQVPTITALIEKLEFIRQKEISKSLSGSLKDLSERQKQAMEDMTTAMMKK
                     ILHAPIMHLKQNTDSEYELVYIAALKRLFDLEEQ"
     gene            4570..5487
                     /gene="hemC"
                     /locus_tag="VF182_07570"
     CDS             4570..5487
                     /gene="hemC"
                     /locus_tag="VF182_07570"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX6436974.1"
                     /translation="MTILKIGSRGSALAMAQAEWVKRKLEETGLHVEINIIRTSGDRF
                     VDHPVQSLGGKGIFTKEIEDALLSGAIDLAVHSMKDLPTEVPEGLAIAAVPQREDARD
                     VLVSRMNIPLEALPDGARLGTGSLRRRSQILNYRSDLILFPIRGNVDTRLQKLFEQDL
                     DAIVLAAAGLKRLGREDRITEYLTPDVCLSAVAQGALAIETRDDKPSRQSVAFLHHDA
                     SFVEVTAERAFLRKLAGGCHVPVAARAFIDGEVLNLTGMVAEPDGRRLCRGQIAGSTE
                     QAAALGTELAERLIREGAGELLAGVITRV"
     gene            5500..6342
                     /locus_tag="VF182_07575"
     CDS             5500..6342
                     /locus_tag="VF182_07575"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX6436975.1"
                     /translation="MAVKEFSTQRADNSVLSGKRIVVTRPRTQAGELSRRLSDLGAEV
                     IEFPTIAVEPPFDYAPMDRAIAQLNQYDWLFFTSINGVQSFFDRLRHFGNDSQDLEHL
                     KVVAIGPETARRLEAEGVHVDLVPSKYQAEGILECLDPAEVRGRQILIPRAAKAREIL
                     PETLRQWGAIVNVVHAYKTVLPQDSQFALRELSEKQIDLITFTSSSTVENFMGLLEGE
                     DLKRISLGIIFACIGPITARTAMDCGLRVAIVSSEYTIRGLVGAIVKFYESPHSKNWT
                     PSGS"
     gene            6358..7332
                     /gene="hemB"
                     /locus_tag="VF182_07580"
     CDS             6358..7332
                     /gene="hemB"
                     /locus_tag="VF182_07580"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX6436976.1"
                     /translation="MHFPAYRPRRLRKNEKLRQLIRETTLTPGNLIYPLFVGPGTNVV
                     RPVSSMPGVAQLSVDRVVVECGEAAGLGIPAVILFGIPETKDALGSEACMSNGVVQQA
                     VRAIKDNVPDLLVVTDVCLCEYTDHGHCGLIKNGAVDNDSTLELLAQEALSHAEAGAD
                     MVAPSDMMDGRVGAIRELLDSRGFDQVAIMAYAAKYASGFYGPFREAAESTPQFGDRR
                     SYQMDPANTDEALREVELDIQEGADIVMVKPALAYLDVIYRVKQQFSYPVAAYNVSGE
                     YSMIKAAGINGWIDEERIMLEVLLSIRRAGADMILTYFAKQLARVLDS"
     gene            complement(7679..8689)
                     /locus_tag="VF182_07585"
     CDS             complement(7679..8689)
                     /locus_tag="VF182_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6436977.1"
                     /translation="MRLFRAYAAAILSIACLLAIPLFAGAQSARINVGYSAISADQLP
                     AWVAKETGIFAQNSIDAQLIFFTGGTTAILALVSNDVPFTQVSGPGLVNSVSAGSDAV
                     FIAAGMTSLNYVLMGKAGVKSGEQLKGGTVAISRFGSATDSIARFALRKIGLTPGKDV
                     TLVQVGSGPDRLSAVLTGRVTAAVINPPSNFIAEKKGLSVVADVAQMGLVFQHTGAAT
                     TRKYIREHPDIVRRYVKSHVEAVHRIWTDKEATVKTLGKYMGSNVDREILEKSRENIL
                     TEALLPKKQIPSLEGLKTVLEDIAERDPRAKTAKPEQFVDFTFIRELEQSGFIDGLYK
                     KK"
     gene            complement(8959..9507)
                     /locus_tag="VF182_07590"
     CDS             complement(8959..9507)
                     /locus_tag="VF182_07590"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(9298..9300),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HEX6436978.1"
                     /translation="MKSIARLIFSLALAMAVVAAASSPARALEVGDKAPDFELESSKG
                     GKLKLSDLKGKNVLINFYVLDFSPTUIKDLQASGSDNYSSFQAENTEVVGISGNAPFS
                     QKAFADFAKITYPLLSDRDGKVMQAFGVYDEPRRVAKRSYVIVDKDGVIRYLNIRPTN
                     SEKDLLSTEELLSAVKKVSKGS"
     gene            complement(9884..10486)
                     /locus_tag="VF182_07595"
     CDS             complement(9884..10486)
                     /locus_tag="VF182_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6436979.1"
                     /translation="MIKLYDFKSSPNCQRVRVVLAEKNLPYETVPVDLRAHAQKTPEF
                     LKLNPNGKVPVVTDSDTVLYESCIINEYLNEKYPTPPLMPQDPAKKAKARILTDYGLT
                     HLESQYQKLRMETVKDAKEQNKDVIAGAQTELRRMLQRLEDEIGDQPYLAGDFSLADA
                     ALIPRFIRLEGFGVLPDKSLPRLGKYIDRMKDRPSVKAIL"
     gene            10624..11148
                     /locus_tag="VF182_07600"
     CDS             10624..11148
                     /locus_tag="VF182_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfopyruvate decarboxylase"
                     /protein_id="HEX6436980.1"
                     /translation="MSAEEAAATKIVTGIKQAGINLIATLPDVNLAQLLYAVDEDPDL
                     LHVPLCREEEGIGICAGAYLVGKKCAAVMQNGGFFNSNNAIISTLLQYQIPLLLLIYY
                     AGDVGDRTFSTSGSMTEPVLQALGIRYYIMRETADAPELIKRAQILAEDSKRPVALLL
                     TKEVLGLRAALKSL"
     gene            11186..11791
                     /locus_tag="VF182_07605"
     CDS             11186..11791
                     /locus_tag="VF182_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6436981.1"
                     /translation="MKRFDCLKLVAEEAKDALVVSSAGAMTLEWNALRPGDGNFRVRT
                     LGLCSSIALGMALGLPHRKIIALDGDGSLLMNLCSLPTIARMRPSNLVHIVFDNEVYE
                     ASGSKKTATACGTDLVGVARAAGIKNSGWANNLSEFGRAILAGMTTNELRFIGAKVTT
                     ERTEVSPYPLDEVENKYRFIRHVEMTEQVEILKKNLPASYV"
     gene            11820..12833
                     /locus_tag="VF182_07610"
     CDS             11820..12833
                     /locus_tag="VF182_07610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6436982.1"
                     /translation="MKIDIEFNSGAQLPMDAIPELARLAEANGFDCAWGGEANNKDPT
                     VMLSAIAAVTTRLKIGSAIYHILGRTPATLALQAVGLEELSGGRFLLGIGSSNPTIAK
                     WHGQVLDQPLGRVREYINIVRAAIRGEKLDFDGNFFTAHNFRMAFKPSGRAIPIYLAA
                     FGPKMTRLAGRIADGVVINMANPAQIRRISDEVRKGAEEAGKNPDDLQIICKIRCSIA
                     GSSAAARDALSHALTYYALADYYRDLLGRMGFAAEVEAMRSAWKAGGFHSARKLVTDR
                     MFADLPLIAATSAHEILEQIRPYAAAGATRIILPYVAASDEVVGEMKGFIEKWSSITT
                     PVD"
     gene            12847..13248
                     /locus_tag="VF182_07615"
     CDS             12847..13248
                     /locus_tag="VF182_07615"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6436983.1"
                     /translation="MPFELLNPAGLESPVGYAHIAKITSGTIVHVAGQAPFDSQGQVV
                     GKGDFVAQFAQVVRNLKTAITAAGGHPPDYAVLTIYITDLQAYLKNKKSLGSVYQEIF
                     GRHFPAITLVEVKSLYNAECMVEISGLAVIS"
     gene            13432..13653
                     /locus_tag="VF182_07620"
     CDS             13432..13653
                     /locus_tag="VF182_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436984.1"
                     /translation="MSYRLTRYNPKPSIEQEREQDLLESVTEEEVIILWRSIKQKGLS
                     EDEAWHAIVTGLALDAAFSNNRRTIQYEQ"
     gene            complement(13696..14187)
                     /locus_tag="VF182_07625"
     CDS             complement(13696..14187)
                     /locus_tag="VF182_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436985.1"
                     /translation="MPSTIAKSVKNFFFPGLSKLNGLHYETAVEGLVLAHELILQLAT
                     QIEAHAAGAPYPHMMRTLQRMASEKYEGARKLRILLEKLGEQPRSATGESKTGINHWE
                     RLNLDLQDQVAVDDLLFTLELKAGETTEIAKLVEELRGTQRSHRRILSDLIALADPQA
                     TQT"
     gene            14555..15520
                     /locus_tag="VF182_07630"
     CDS             14555..15520
                     /locus_tag="VF182_07630"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HEX6436986.1"
                     /translation="MTVDDKTLPIDLPFLNIELSKQEDLKDADSKEKALVPFDPLQRY
                     LAEIRRFPLLSREEEHKLAVEFKEFGNIEAAYKLVTGNLRLVVMIAREYQKALKSLLD
                     LIQEGNMGLMEAVKNFDPYRGVRFPSYAVWWIRAYIIRYIMNDWRMVKIGTTQAQRKL
                     FFNLQKEKERIEAEGLTPGPKLLAQRLNVKEDEVVEMEQRLANRDLSVDMPIGEDEEA
                     TMLNFLPDQKQNPEEHFAESQYRELLQQKMAEFARTLKDKELVIFRERLLNEDPITLR
                     EIGEKYNISRERVRQIEERLKKKLKTYLSKELDDVNDGKTVKTAD"
     gene            15629..16081
                     /locus_tag="VF182_07635"
     CDS             15629..16081
                     /locus_tag="VF182_07635"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX6436987.1"
                     /translation="MQFEKQVEIQAPREKVWNFIWDVDQFIACVPGCKEAKTVEEGKR
                     YSATMVEKVGPFKVEFPTTIEVLERDELSRIKAQASGADNKIGSRMKLDLEVKLREED
                     NKTILQFVAGVDILGKLAALGHGIIKRKADQVLDEFAQAVKKRLEGDS"
     gene            16083..16949
                     /locus_tag="VF182_07640"
     CDS             16083..16949
                     /locus_tag="VF182_07640"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6436988.1"
                     /translation="MLRRFRLEEPTSVAEVSELLGRFGDSAKIYAGGTELLLAMKEGL
                     VQYERLVNIKGVAGLNEVKQNNGEVHIGALCTHRELENSPLAKEKLPSLVKLEENVAN
                     VRVRQVGTLGGNLCFAEPHADPGTLLIAFGAHLVAEKGDGKREIPAESFFVDAYETAL
                     EPDEVLTRIVVPVPAQPSGICYMKFGYLERPSVGVAVAVKLNSGKTAIDDIRIAVGCA
                     GPAPKRVAEAEAFLKGKSLDDATQKIPEAGETAGRVSQAISDLHGAQDYKEHIIQVLL
                     KRSFQNALSQCT"
     gene            16967..17479
                     /locus_tag="VF182_07645"
     CDS             16967..17479
                     /locus_tag="VF182_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6436989.1"
                     /translation="MAMTTLRRSDLQTLPISFTLNGKLQELDVEPHELLLDVIRDRLG
                     LTGAKRSCDVQVCGACTLLVDGRPVSACTTLAFEVRGRSVMTIEGLAENGKLHPLQQA
                     FIEHGGFQCGFCTPGMILAAKALLDQNPHPTEEELKHFMHGNICRCTGYKKIIESIMA
                     AAKNISEATH"
     gene            complement(17916..18839)
                     /locus_tag="VF182_07650"
     CDS             complement(17916..18839)
                     /locus_tag="VF182_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6436990.1"
                     /translation="MSVVFWKELADHFSSRRFMILIFIIVLTGLWATYATGQAIRQNT
                     ETVPSQYAFLLLLTSSGETLFSLATFLGFFGPLVGITLGFDSISGEYARGTLSRVLSQ
                     PIYRDSFINGKFFAGLTTIAVLWASILLLVIGLGITLLGFPPNGEELWRMLIFAVVGI
                     FYIGFWLALAMLFSLLFQKTVTAALASIAVWLFMTLFVVIVSQGIASIVVPDTSTPES
                     VARRADIENIIGRISPSTLFSETVHILLNPAARVFGLALQSQAEGILPTPLALGQSLT
                     LIWPHVTTLFGLVAVCFGISYIFFMRAEIRA"
     gene            complement(18842..19774)
                     /locus_tag="VF182_07655"
     CDS             complement(18842..19774)
                     /locus_tag="VF182_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6436991.1"
                     /translation="MSAVVDTKNLTKRYREKLAVNTLTLSVQQGEIFGFLGPNGAGKT
                     TTILMLLGLTEPTSGELSVCGYNPTHDPLEVKKRVGYLPENPGFYDDISAQENLLYMA
                     RLNRIPEKEAQRRTAEVLEQVGLSEDGRRLVREFSRGMKQRLGIAEVLVKNPIAIILD
                     EPTLGIDPDGAIRILELIKNLNRDHNLTVMLSSHQLQQVQQICSRVGIIVKGRLIVQG
                     EMDELGRAILKGRQWNFLVEVGGNRDSLDRSLQSIDGIDEIEKRSQGWFLRCTKDVRP
                     EVVSLVTTKGLPLLQLRSEDPTLEEIYLKYFREA"
     gene            complement(19771..21021)
                     /locus_tag="VF182_07660"
     CDS             complement(19771..21021)
                     /locus_tag="VF182_07660"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HEX6436992.1"
                     /translation="MSFVRLRVRLRMMLVLFSWFLLCSASLGTSAVAAEKAAESTGQK
                     DAPSSKESFNLIIPYKDLTVGQGQEVTMDTEVVNRTKNPVEVNLSLESIPKGWEANFN
                     SRYPSYPVRSVTVQGEKSTTIEFKSKVPQNTKPGNYDIKVVAKDTEGPTSYSDTINFR
                     VTSAKVATGGLRLTSQYPVLTAPSGQTLKFTIDLKNETNKPLPVSLTAQTPPGWAVRF
                     KPQFGDTQYSSILLKENGSETVAVEIDTPANAEAKEQPVKVVARSGAFEASADLKVNL
                     KGTQDLKMGSLAGTLNTSVTAGNRTPIDFVVANAGTAPIRNLNFISKKPSDKWTVEFR
                     PDKIDALNPGEVREIKMDILAPDRTIAGDYLMTLTANSPEVNRSVEFRVTVSTPTLWG
                     WVGFGIVALVVLGLVIVFFRLGRR"
     gene            complement(21101..21553)
                     /gene="trmL"
                     /locus_tag="VF182_07665"
     CDS             complement(21101..21553)
                     /gene="trmL"
                     /locus_tag="VF182_07665"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008308219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEX6436993.1"
                     /translation="MHVVLFQPEIPPNTGSIARLCAATLTPLHLIEPLGFKIDDRHLK
                     RAGLDYWEFVDLRVHKSWDEFLAAASMKQFLYFSKRATRSYTTARYQEEHYLVFGPET
                     RGLPQDLLEHNPESSYRIPMAGAGVRSLNLSNAVSIVLYEGLRQLGKA"
     gene            complement(21544..21993)
                     /gene="dtd"
                     /locus_tag="VF182_07670"
     CDS             complement(21544..21993)
                     /gene="dtd"
                     /locus_tag="VF182_07670"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX6436994.1"
                     /translation="MRAVIQRVSKAQVTVGENIVGEIASGLCILLGVGRDDDLPDADF
                     LSEKIKNLRIFEDQEGKMNRSLLAIRGEVLVVSQFTLYAGSRKGNRPSFTDAAPLAQA
                     EKLYHHFIKRFRDAGVSVETGQFQARMKVSLVNDGPVTFVLDSRACT"
     gene            22199..22951
                     /locus_tag="VF182_07675"
     CDS             22199..22951
                     /locus_tag="VF182_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6436995.1"
                     /translation="MKQIKILCMLTWLVFALKPGSGTAESPDFHFRPAQPIAIHGQLR
                     GSVLLSGKYQPPDPLKVVKSRSFCGLSVPNETLIVDQSGGLRNAVVILRPIDRTVKVY
                     PSRILLDNKQCAFIPHVQIAGIGSELLLKNSDPILHTVHARLGKETLFNVGLPRWRQV
                     SKILDRVGVIRIDCDVLHTWMTAAIVVVSSPYFAVTDETGFFNIQEIPAGKYELEIWH
                     EVLGSKTAQISIPQSGTILLDAIYNRVPEIKS"
     gene            23000..23782
                     /locus_tag="VF182_07680"
     CDS             23000..23782
                     /locus_tag="VF182_07680"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX6436996.1"
                     /translation="MYPIIFQLGPITIYSFGVFMALAALSAAWVVHSELKRRGYDPEL
                     ASSMIFAAAVGGLIGARLLFILGEWHDFLNSPSSYIFTGAGFTWYGGLFGGAVAVSWI
                     VRKNEIPWLVGADIAAPALALAYGVGRLGCHFAGDGDWGSITKAPWGVAYTNAIIGWV
                     DPMTGIPYPAGVRVHPTPIYEFLQSIVIFAILWSIKTRGLAPGTLAWLYLVLAGLARF
                     TIEFWRVNPILAAGLSEAQWISLILIAVGLAMLVSRRVRLAY"
     gene            complement(23874..24989)
                     /locus_tag="VF182_07685"
     CDS             complement(23874..24989)
                     /locus_tag="VF182_07685"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX6436997.1"
                     /translation="MTSRKQIAAIQNCIGNAIDASLIHNKQIAGIIGDAPSSYSRSPA
                     LWNAAFNYLGINAVYLPFDVAEPRLGELIAILKGLDNFLGINVTVPHKIRVMDFLDEI
                     EAGAKRVQAVNTIVKSSSGKLVGYNTDGSGFVESLLTPLPEETESFLTSLAGTEVLLI
                     GAGGSARAIAFRLSDMLAPGQLVIANRNLEHALSLSAELQKNGGKAQAIDETEIGPWA
                     RRVGLIVNTTTKGQGGIRKLPNGELTSLEPYSALASAHPVSITESDFAGSQTRQKWVC
                     AARADIDENNQASMTLARAIAPNVRFYDLIYHPEETVFLRHGRLSGHLTKNGKAMIIF
                     QAAIALCSHVCKQHLIESGKDVAAIYRAVCQVMSESW"
     gene            complement(25060..26487)
                     /locus_tag="VF182_07690"
     CDS             complement(25060..26487)
                     /locus_tag="VF182_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX6436998.1"
                     /translation="MDYTIEDLEAWNSRILEIVHQFGLDPYPQEFEICDHEDMLTYMV
                     YSGMPSHYPHWSYGKSFEKLKTLYEYGVSGLPYEMVINSNPSIAYLMRDNSLALQVLT
                     IAHVYGHNDFFKNNFTFGATRAKYTIETFKSHANRVRHYVEDPSIGLERIERILDAAH
                     ALSLQCRRNLSIRKLSSQEQIETKVQDAQPPADPFGAIHRRQDYVSPDLNKVPLYPEE
                     DLLIFIRDHNPHLADWEKDLLTIVHEQSQYFIPQIETKIMNEGWASFWHKRILETLEL
                     PQNLHLEFLVRHAQVLRPAPGGLNPYHVGMKIWENIEKRWDHPSAEDEKEYGRRNKSG
                     AEKLFEVREVERDASFIRRYLTEELVRDLNLFEYRTRGNEQVVSRVADDLNWRQIKET
                     LIQNVGTSTIPMIKIEDADYNKNRTLFLRHHHDGRDLQLEYAEKTLQYLNQLWGHEVA
                     LETVLDSRKSLLCYSDNRLAIKNGI"
     gene            complement(26513..27733)
                     /locus_tag="VF182_07695"
     CDS             complement(26513..27733)
                     /locus_tag="VF182_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEX6436999.1"
                     /translation="MTVMRDPQTGIDRTAETIFRPYNSSSGQRSDRSAGDRLRHRQKV
                     REAIRNNIADIIAEESIIGKDKNRIIKVPIRGVKEYRFVYGDNAPGVGQGGDQEPQPG
                     QVVGQGEKGKQPGQPGDQPGVDYYETDVRLDELIELMFEDLELPDLERKRLRQIEVEK
                     QFRQKGYRRKGVRVRLDKKRTARSRLKRKKASQRRDLMGARSRFPFHKDDLRYHHSVN
                     DLRRESNAVVLCIMDTSGSMDTMKKYLARSFFFILYQFISTRYQNVEIVFIAHHTEAQ
                     EVTEEDFFHKGESGGTFISSGYNKALEIINERFHPALWNVYVFHCSDGDNFESDNPAA
                     LKAATELARSSNLFGYGEIKPLGAGYYGSSLIPLFSQLDEPNFHTLQIHRKEDIWTSF
                     KTFLAKDRGREDAA"
     gene            complement(27738..29672)
                     /locus_tag="VF182_07700"
     CDS             complement(27738..29672)
                     /locus_tag="VF182_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HEX6437000.1"
                     /translation="MDHNKEFQELIQKDRDLHQSQSWRGNLLGYLDKVRDDPGVAKLA
                     HARLYDVLIKAGVRDINVDDDPHAKRIYKDESIKVYDFFADEFFGIEKTISQIVRYFH
                     AASLKGEESRQVLYLMGPVGSGKSSLVEKLHRGLEESPPFYAIEGCPMFEEPLHLIPR
                     HLRKEFEKMLGVHIEGDLCPVCRFKLQHEYGGKYEEFPVSTIEFSKRARVGLGVVPPV
                     DPNNQDTSVLIGSEDISKLDLYSEGDPRVLELNGALNVGNRGMVEFIEVFKNEIEYLH
                     CMITATQEKVVPAPGRHGLVYVDTVIIAHSNEAEWQKFKADHTNEAILDRIVVIRVPY
                     NLRLSEEVKIYQKIIRHSDFRAHVAPHTLEIASMFAILSRLEPTNKCDLMTKLRLYNG
                     EEVVEKGRSKKVDVQELKEGVKREGLSGISTRFIMKALDNALSDNTTGNCINPINVRE
                     ALINMVKEADLPDDTRKQYLELLQDALHKEYLEVLEKEITRAFVYSYQEQAEALFQNY
                     LDHAEAYVNKSKVKDRNTKEELSPDESFMKSIEEQIAIIGSAADGFRQEVIAYLWAAN
                     RRGEKIDYKSYEPLKEAIEKKLITSVRDLSRVITKSRMRDEEQSEKYNVMVKNLLENG
                     YCPSCVDVILKYAANNLWKD"
     gene            30162..30686
                     /locus_tag="VF182_07705"
     CDS             30162..30686
                     /locus_tag="VF182_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HEX6437001.1"
                     /translation="MKPGELMIQPGLRWYAIQTKGNKEREVAKRLTDLKFEIFLPWLR
                     DRRRIGSKYHWVMVPLFPGYLFCRLDIVVSGKTARYSPGVKDFLRFGNEITEISNDIM
                     VALQERCPEGVAAVNPVSLTAGDSVTINEGPFAGLQAIFEKKMKGSERVAVLLELLGR
                     QTRLILPSETVNRT"
     gene            30836..33358
                     /locus_tag="VF182_07710"
     CDS             30836..33358
                     /locus_tag="VF182_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6437002.1"
                     /translation="MRHQAWHRRGLLVIFAAFAFVIVCGTVLSSVQWISKPFPGFFVH
                     ENLTVGPYSLPHWSGGVAGLQSLDLIIKVDGRPVSGRAEFYERLRNLSVGTRLTYTIG
                     RRSNIVELTIPSMMLTWQDWFLSFGVYVFTGLAFFAIGVAPYCFRANSPAALPLSFMV
                     IAVFVWFESTFDFMTDGFVPKEMRLFAMILTPSAGIHLALLLKQGRPVRHTKPFLLVF
                     IYGTALALGALSSITFYGPVDRWIDIFRINYLYACIGALAFLSIIGSALRKTPTALER
                     SRLRVMFVGAVLGFLLPTVATVLTSTFQWAIPYNLAMISTVFFPLSVAYALLKYSLFD
                     LGNTLKTGLTRLSLMVFLLALYAAIFFLVGPSVGVYDKDPLTPLFFSMLVVLIFNPVL
                     RGIETLVDRYIYRQEYDPVRAQRDISLFLRSLTTARVIANGFLEQVTSILGVKKAVLI
                     YRSAAVPNDLTAATDGNEVKFAPLLDRARLLWRAEHVAGFRGLSRSEVLANPVFESER
                     REWLAMFEPLASELLIPIVFEHEVRGLVSYSAKRGGREYRGDDLTLLGTLTEQLALSL
                     ENGIRYEQSEKAKDEYHRLYDEAERAKRKLIDEDRIKKNFVANVCHELRTPVSTLIGF
                     SEILLNRELPADTRDILKTMLSNGQDLSNMMDGLLDFSRMEADAVPNSFERINVKDLL
                     GGLELMTQRLIRGRPIQFRMNIEDAIQTINSDPRKLQQILVQLLTNALKFTEKGAVEV
                     KIATMTDQSGGFLEIAVSDTGIGIDKNDQEIIFDSFRQLDGSSKRRFGGTGVGLSLCS
                     KLAAALGGKISVKSELGLGSVFSVFLPLTSVVGTSQHAGSKA"
     gene            33417..34067
                     /locus_tag="VF182_07715"
     CDS             33417..34067
                     /locus_tag="VF182_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437003.1"
                     /translation="MMTSKDFLKELLAMVNDFCRTLDAQPPAYSYIPLRTDEERIRVM
                     KSRLYNEVRAAELFGTWLKTTPELEVKAIMAESSHEEMEHAQLLSKRIANFGFEPFDY
                     LPLPAQMAMFNAIEGLTDTCQRMAGFSLAGEAVATYLTQKSLQASTVPDWIKQPYRKI
                     TQDEEGHGSIPQEILQRYATTEERQERARRAVAMRLVLFREYLNSLDRWAMGKDRW"
     gene            complement(34073..34381)
                     /locus_tag="VF182_07720"
     CDS             complement(34073..34381)
                     /locus_tag="VF182_07720"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6437004.1"
                     /translation="MNELDNEARKQAVIDNLRPICLCNKIRKGIVVKAIQAGAKTFEM
                     VSKRTGIGTGPCGAKRCGAAVRGLLGEPVTTCAECGWAILLGPGPLICPRCEAVQSER
                     "
     gene            complement(34407..>35209)
                     /locus_tag="VF182_07725"
     CDS             complement(34407..>35209)
                     /locus_tag="VF182_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX6437005.1"
                     /translation="FSLTDVSAKYLKRDAPGYLGGMIRFEASLWHCWMSLTEAIRGGR
                     PVRPADMYQDDPKETAIFIDAMDTLVKARGDAEIMAGAFDWGNARELLDVGSGPATYP
                     IALCKRFSNLRATIFDLPATLALTGSYVQKAGLGSRMRLISGDYRVDEIPGSYDAILL
                     SNIVHGESFEVNQRLMVKLSAVLKPGGSVIVKDHILDSTRVNPTVGAVFSLLMLLTTV
                     AGRCYAFDEVKSWMLSAGLMHIKQIDLPPPLNSSLIIASKEDSIKKSC"
BASE COUNT         8318 a         8869 c         9334 g         8688 t
ORIGIN      
        1 caggctggcg cgcaggatac gcgtgaggat agccgccatg gccattccga gtgtgagcga
       61 cggtaaaatc aggtgcgaaa agtcgccgcg cccggagacc ggcaaccagc caagttgaat
      121 ggaaactagg atcatcagca atggacccag ccaaaagttt gggatcgaga gcccgagaag
      181 cgagaaaaac aacaccacac gatcgatcca agtatcacgt ctaagagcgg caagtgtcgc
      241 aaggggaaac gcaatcacaa tggcgacaat catggcggag atcgtcagtt ggattgtggc
      301 cggcacgcga gcggcaatga gttcggcaac ttcgacgttc tcataaaggg aatgccccaa
      361 atcgccgcgc catagatcgg cgagaaaact tgtgtagcgt gtcagtaccg gttgatcgag
      421 accgagctgg cggcgtagtt cttgtttgtc agtagcgctg gcggtctctc ccagcattac
      481 ttcgaccgga tctccaggaa ccagattgat caggaaaaat accagggtca aggcgccaaa
      541 taaggtgggt agaagcagca gaagccgatt tagaaaatat cgcttcacgg caggtgcggc
      601 gaatggctca atgaggtccg tctcaatgca aacagatcgc catccggata gggtgtaaag
      661 ccgaccacct ggggcttgcg cacgactata tttttcaccc accaaagcgg gatatacggc
      721 agatcttgat taattatttg ctgcacctgg tggtactgcg cttttcgttg tcttcgatca
      781 gactctcgcc ggccctgttc gagcaaatga tcgacaacag gattgccata acgaccgcga
      841 ttgtccccat tgggaggtac gctcgaggaa tgaaaaatat tgtaataaac gtcgggatcc
      901 actatgccca cccatgcgag cgagtaaaga tggaagttac cttttttgat atcactataa
      961 aacgttcccc actcataggt gcgcacctcg agctcaatgc ccacccggct taattgttcc
     1021 ttcaaggtct cggcaattct tctgcgcaaa tcgatgttcg tagtcttaaa agatagcttg
     1081 aaacgcggcc gcggcccctc tccgtcggga tccggatagc cagcctgatc caacagctgc
     1141 tgggctctgt ggggatcaaa gcgccaacgc gggacagagt cgtcatgcgc ccagttaagc
     1201 ggcgaaagca gcccattggc ggctgtcgct tggtctttga gaatgtgccg gatgatgccg
     1261 tcacgatcga tggagtgggc taatgcttgc ctgacgtctc ggtttctcag tatcggatgt
     1321 gtgaggttta tgccgatgta ctgaaatgtg gttcccgggt tggtctcgac aactgcatcg
     1381 gtattttttt ccagccatgg aagggtatcg ggctcgatat cgttctgcaa aagctggatg
     1441 gttccttttt ggaactctaa aacccgaacg atggcatcgg gcacaatttg aaagaccagt
     1501 cccgccaagc gaggttgccc ctcccagtac gacgcgtttg cttttagaga cactttctca
     1561 cctggattga tctctgccag tgcaaacggg cccgaaccag gtggcgcctg ttgcctgcca
     1621 ttctcccgtg gtacccggct gtcgaccggg actatgccaa tcgtgcaatg ctcaagaaaa
     1681 ggggcgtgcg gttcggataa atgaaagcgg atctcgtcgc ttcctaactg ctcaaccgat
     1741 tctagaaccc gcagcgatcc cagcttcggt gagcgattgc gtcgatccat aattgagtca
     1801 taggtgtact ttacgtcccg ggctgttaac gctctaccgt cgtggaaggt gacgcctcta
     1861 cgaattcgga agctataggt ttgtgcgtcg atcatcgtcc accgttcggc caggtctccg
     1921 acgaggcgtg atttctcatc caggcgaaga agcgaactat agattagcgc actaatgcgg
     1981 acagagtttg catccgtagc gtagcgcgga tctagctggc ttgggctgct ctcgattccg
     2041 atcaccaaat cgttcaagga ggggttggcc cggggtccta ccctgcagcc agtaaacaac
     2101 gtgccggtga tcgtcaataa agagaaaaga agaaccttcg cagcaaacaa cttggaagtc
     2161 ccacgtcaaa tctgaatgag aatatcgatt ttaccggatg gggtcaattt aattgatcga
     2221 aatttggtta agtgatcgaa attgggcgat gagatgagag atgatccaaa gatctccggc
     2281 cagtcttgca ttcgtcaagg gctcggtttc acgggaaagc gattcgccct caaatatcca
     2341 gaagtttagg cgtacggttg accgacgtgt cgcttgaagg gtgagaaggt tagcttataa
     2401 tgacggtcgg tatgagtttg atcctgctta aactgaccgc gatactctat ctgattgcga
     2461 ccggaagttt tgtcttctac gtcgtccgcc gtgacaccgc ttccaggctg ccgccgttga
     2521 tcttgggcgc tggatttatc gttcatacct tggcattgag cgcgtatttt atgcgcgaag
     2581 ggtttccggc gctaaccgaa atgcgcgaag cgctactatt taagagttgg ttgatggtcg
     2641 catgctatct cttgatccag ataaagtacc gacttaccgt tctcggcggg atcattgcgc
     2701 ccctggcatt cttaatgtct ctggccggta tcgcgttcgg tagcggaagc ggagaaattc
     2761 cgcctgatct gaacaccttc tggttaccgc ttcatgtaac atcggcgttc ctggggaacg
     2821 cggtattcgc tttggcgttt agcgtcagct tgatctatct gcttcaggag cgccatctca
     2881 aacataaacg tatgaccgcc ctgatgaagc gctttccatc gttggaatct ctcgatcggc
     2941 ttaattatgt cctactcgga tgggggtttc cgttcatgac tctagggatt attaccggca
     3001 gcatctgggc aggcatctat tggggtaact attggtcgtg ggaaccgcgg caaatctcat
     3061 cgggcatcgc ttggcttttt tacggcgcgc ttctgcacgg gcgcatcacc gccggcctgc
     3121 gaggcaaaaa ggcggctttt ctcacgattg tcggatttac cgtggttctc ggttatttct
     3181 tgtggggaga tgcaattttt cccgggcggc atggaggtcg gttcgaatag ctcgtgcata
     3241 cagaaatatt catagttggc ctcaaccacc gtagcgcgcc catcgaagta agggaaagtg
     3301 tcgcctttga aaaatcccag gttacggaag cgctcgcaca gcttcgtaaa atctcggagg
     3361 ctcgggaaac cgttatcctt acgacctgca atcgcgtcga agtggttgct gccgcgccgg
     3421 acaccgatca tgccatggaa ggggtcaaaa gattcctcga ccaacaacga tcaccttcac
     3481 ataatggcag cctcagcgac catctttata gttaccgcgg tgccgacgcc gtccggcact
     3541 tgttccgcgt cgccgcaagt cttgactcca tggtagttgg cgagccgcag attcttggcc
     3601 agttgaaaga gcactataac gcggcgcaaa aggccgggac tgtcggtgcg gtactccatc
     3661 ggctgtttca caggtctttt tttgttgcca aacgagtgcg gaccgaaacc gcgatagcca
     3721 gtcgcgccgt gtccgttagt tcggttgcgg tggaactggc agggagaatc tttgatcgct
     3781 ttgacgagaa aacggttatg ttgattggcg cgggcaagat gggcgatctg atggcgcgcc
     3841 atctgcagcg ctgcggagta aagagcatca tggtgacaaa ccgcaccttt gagcgcgcag
     3901 tagaattggc ggaaaagatt cacggaaatc cgattcgata tgatgatttc ccgcgatatt
     3961 taaaattggc tgacttggtg atcgggtgcg cgggagcgcc ggaaacgctg gtggatgccg
     4021 ggatggtgga caaggtgctg cgggaacgca aacaggcgcc gatgttcttc atcgatattg
     4081 gcgaccgaag gaatttcgat cccggcatca acgccatcga taacgcttat atctataaca
     4141 ttgacgatct gaagagcgtg gcggaagaga atttaaatga aagatcaagc gaggccgaga
     4201 aagctgagtc cattgtgcag gaagaggtcg gaagttttct tgattgggtc agttcactag
     4261 agcaggtgcc gacgattacg gccttgatag agaagctgga gttcatacgc cagaaagaaa
     4321 tcagcaaatc tctgagcggc agtcttaagg atttgtccga aagacaaaaa caggcgatgg
     4381 aagacatgac cacggctatg atgaaaaaaa ttctacatgc gccgattatg catttgaagc
     4441 agaacacgga cagcgaatat gagttggtgt atatcgcggc attaaaaaga ttgtttgact
     4501 tggaggagca gtgagccgcc acggatcgtc tcatcggttt gccggctccg gtagcgaaag
     4561 tttggtccga tgactatctt aaagatcggt agccgcggta gcgcattggc gatggcgcaa
     4621 gctgaatggg taaaaagaaa actcgaggag acgggtctgc acgttgagat taatatcatc
     4681 agaacaagcg gcgaccggtt tgtcgatcat ccggttcagt ctctgggcgg caaaggaatt
     4741 ttcaccaaag aaattgaaga tgcgctgctc agtggcgcga ttgaccttgc cgttcattca
     4801 atgaaggatc tacccactga agtacccgag ggtttggcca ttgctgcagt tccccagaga
     4861 gaggatgctc gcgacgtgtt ggttagcaga atgaacatcc cattggaagc tttgccggac
     4921 ggcgcaaggc ttgggactgg cagtcttcgt cgcagatcgc agatactaaa ctatcgatct
     4981 gatctaatac tgtttccgat tagaggaaac gtcgataccc gtttgcagaa gctattcgaa
     5041 caagacttgg atgctatcgt gttggcggcc gctgggttaa agcggctcgg tagagaagat
     5101 cggattacgg aatacttaac ccccgatgtc tgtttgagcg cggtcgccca aggagcatta
     5161 gccatagaga cccgtgacga taagccatct cgtcaatcgg ttgcgttcct gcatcatgat
     5221 gcaagttttg tagaagtcac ggctgagaga gcgtttttac ggaagctggc cggaggctgt
     5281 catgtgccgg tggcggcgcg cgccttcatc gatggcgagg ttctcaatct tacaggcatg
     5341 gttgccgaac ccgacggccg ccgcctttgc cgcggtcaaa ttgcaggctc cacagaacag
     5401 gcggccgcgc tgggaaccga gttggctgag cggctcatac gggagggagc aggggagctg
     5461 cttgccggag tgataacccg cgtatagcgc aggcggatta tggcagttaa agaattctca
     5521 acgcagcgag ctgataactc agttttgtcg ggaaaacgaa ttgtcgtcac ccgaccgcgc
     5581 acccaagcgg gggagctgtc gcgtcggtta tctgatctag gagcggaggt catcgaattc
     5641 ccgactattg ccgtcgagcc gccattcgat tatgcgccga tggaccgcgc tatcgcccag
     5701 ctaaatcaat atgactggtt gttcttcacc agcatcaatg gcgtgcagtc attcttcgat
     5761 cggctgcggc acttcggcaa cgattctcag gacctcgagc atctgaaggt ggttgcgatc
     5821 ggaccagaaa cggcgaggcg tctggaagct gaaggcgttc acgtcgatct ggtgcccagt
     5881 aaataccagg ccgaaggaat ccttgaatgc ttggaccccg ccgaggttcg cggtcgtcaa
     5941 atcctgatac cgcgcgccgc taaggcgcga gagatattgc cggaaactct gcgtcaatgg
     6001 ggtgccatcg tcaatgtcgt ccacgcttat aaaaccgtgc tgccgcaaga cagccaattt
     6061 gctttgcgcg aactatctga aaagcaaatt gacctaatta cgtttacgag ctcaagcacg
     6121 gttgagaatt tcatgggcct attggaaggt gaggatctta agcgaatatc gctgggaatc
     6181 atttttgcct gtatcggacc catcaccgcg cgaaccgcca tggattgcgg tttgcgggtg
     6241 gctattgttt cctccgaata tacgatccgt ggactagtcg gtgcgattgt taagttttac
     6301 gaatcgcctc atagcaaaaa ttggacgccc agcggatcgt aaggagcttt tgcaaacatg
     6361 catttccccg cttatcgccc cagacggtta cgcaagaacg aaaagctgcg gcagctgatc
     6421 cgcgagacca cgcttacgcc gggtaacctt atttaccctt tgtttgtcgg accgggaacg
     6481 aacgtcgtcc gaccggtttc ttccatgccc ggcgttgccc agctctcggt cgatcgagtg
     6541 gttgtcgaat gcggtgaggc tgccggcctc ggaatcccgg cggtaattct atttggtatc
     6601 cccgaaacta aggacgcgct cggctcggaa gcctgtatga gtaatggtgt ggtgcagcaa
     6661 gccgtgcgag cgatcaagga caacgttccc gacctattag tcgttaccga cgtttgcctt
     6721 tgtgaataca ccgatcatgg ccattgcggc ttgatcaaaa acggcgctgt agataatgac
     6781 agcacgttgg aacttcttgc tcaggaagcg ctttcccatg ccgaggccgg agcggacatg
     6841 gtcgcgccgt cggatatgat ggacggcagg gtcggcgcaa tccgtgagct tttagatagc
     6901 aggggcttcg atcaagttgc cataatggct tacgcggcca agtatgcatc tggtttctat
     6961 gggccgtttc gagaagcggc ggaatcaacg ccgcaatttg gcgatcgccg ctcgtatcaa
     7021 atggatcctg ccaataccga tgaggcgttg cgggaagtcg aactcgacat tcaggaaggc
     7081 gccgacattg tcatggtcaa gcccgcgctg gcctatcttg atgtcattta ccgcgtaaaa
     7141 cagcagttta gttatccggt tgctgcgtac aacgtcagtg gggaatactc aatgatcaaa
     7201 gcggccggca tcaatggctg gatcgacgaa gaacgaatta tgttggaggt tcttttatcg
     7261 atcagacgcg cgggtgccga tatgatcttg acctatttcg cgaagcagtt agcgcgcgtc
     7321 ttagattctt aggaagcagt cggtggcgtg ttctaatcgc aagccactaa tccagcggaa
     7381 ggtgttgtgg acgtcatccc tcaaattaaa tttgatagat ccttgatctt tcgctgaagt
     7441 gtgcgctcgc attccgcttc tctccctgag acgtgaacct gagattccga aagccggaac
     7501 agcaggatcc tcgaaccttt tgcgagccgt aggagaaacg tattggagtc accgtcgtag
     7561 tcgacatcta cgctcacttg ttcatctact tgccgggcaa cacggttggc ccaatccgca
     7621 acctcggaag ccgccgcctc acctcgtttt tcagccaaca tcaactgcca cgttgacgtt
     7681 attttttctt gtaaagcccg tcgataaagc cgctttgctc gagttctcgg atgaaagtaa
     7741 agtccacgaa ttgctcgggc ttagctgtct ttgcgcgcgg atcgcgttca gctatatctt
     7801 ccaagaccgt ttttaggccc tcgagactcg gaatctgttt tttcgggagc aacgcttcag
     7861 tcaaaatatt ctcgcggctc ttctccaaga tctcgcgatc gacattgctt cccatgtact
     7921 tgcccagcgt tttaacggtg gcttctttat cggtccagat tctgtgaacg gcctcaacgt
     7981 gggatttgac atatctgcgg acgatgtcgg gatgttcgcg tatgtatttt cgggtcgttg
     8041 ccgcgccagt atgttggaag accaagccca tttgggccac gtccgccacg acgctcagtc
     8101 cctttttctc tgcgatgaag ttggaaggcg gattgatgac tgcggcagtg actcgcccgg
     8161 tcaacaccgc gcttagccgg tctggtccac taccgacctg taccagagtg acgtccttgc
     8221 ccggggtgag gccgattttt ctaagcgcaa accgcgcaat cgaatccgtt gctgaaccga
     8281 agcgactgat tgctaccgtc ccacctttaa gttgttcgcc gcttttgacc ccggccttgc
     8341 ccatcaaaac ataatttagc gatgtcatgc ccgcagcgat aaacaccgca tctgaaccag
     8401 ccgaaacgct attgaccaaa ccaggtcctg acacctgcgt aaacggaaca tcgttggaaa
     8461 ccagcgcaag gattgctgtg gtgccgccgg tgaaaaaaat cagttgggca tctatgctat
     8521 tttgagcgaa gattccagtt tccttagcaa cccacgccgg gagttggtcg gcgctgatcg
     8581 cgctataccc gacattgatc cgggccgatt gtgccccggc gaataagggg atagctaaaa
     8641 gacatgctat cgataatatt gcggcagcgt atgctctgaa aagtctcatt gggtcctcca
     8701 ggaagaatct aagtaaatag tactagacga ccaaactcta aacaagcaag cttcccgccg
     8761 ccggagaggc ttgctcattt acgagtcgag taaaggctta aacaaagcaa agtttattgc
     8821 gtcacgtact gatcaaattt tggcttgacc gatcacgtcg gcctacccag gctgcgagaa
     8881 agagccaagg gatgaaacta ttgcggccat tagataggtt gaattactaa caatagtaat
     8941 tctccttccg tgacaatctt agctcccttt gcttaccttc ttgaccgcgc tgagcaattc
     9001 ttcggtcgag agaagatcct tctcgctatt ggtagggcga atgttgagat agcggatgac
     9061 tccatccttg tcgacgatga cataagaccg tttggctacc ctcctcggct catcatagac
     9121 gccgaacgct tgcatgactt tgccgtctct gtcactcaac aacgggtatg taatttttgc
     9181 gaagtcggca aaagctttct gtgagaacgg agcgttacca ctaattccaa cgacttcagt
     9241 attctctgcc tgaaacgacg aataattgtc cgaaccggac gcttgcaagt ctttgattca
     9301 ggtcggactg aaatccagaa catagaagtt gatgaggacg ttcttacctt tgagatcact
     9361 tagcttaagc ttaccgcctt tgctgctctc aagctcgaag tcgggcgcct tatcgccgac
     9421 ctcgagcgcc cgcgctggcg aacttgcggc ggcaacaaca gccattgcta atgccaaaga
     9481 aaatatgagt cttgcgattg atttcatcgc ttcccccttt tgacgagaac ttcgggtcag
     9541 tattattccg aattccgata gtttccgctt tatgtctcag ttcacgggta ctgtcaaccg
     9601 caaatgagcc ccaaataaca gacattagcc tgactcgctg tatgtggctg tcttcatcaa
     9661 ctcaccgccg ttatctccga ttattcagtc caactgatgg tcgaagcaga aacggaattc
     9721 ggctcgctta acacatgagg cgccccttga gactgctcct taacgtaata tgttaccgag
     9781 tcgtacatat gagacggcga tgaatcgccg aacatcaatc caaaaattac ttctccactt
     9841 catgaatgct gaggcgtgag tcattaaaaa agctcgcgcc gaattataga atcgccttta
     9901 ctgacggtcg atctttcatc cgatcaatat attttcccag tcgcggcagc gatttatccg
     9961 gcagcacgcc gaaaccttca agccgaataa agcggggaat cagtgctgcg tccgcaagtg
    10021 aaaaatctcc tgccaagtag ggttgatcgc cgatctcatc ttccagtctt tgcagcattc
    10081 ttctaagctc ggtctgcgcc ccggcgatca catccttatt ttgttccttc gcatccttca
    10141 ccgtttccat gcgtagtttt tgatattgac tctctagatg ggttagcccg taatcggtca
    10201 agatgcgagc tttggctttt ttagcgggat cctgcggcat gaggggcggt gttggatatt
    10261 tctcgttgag gtattcattg atgatacagg attcatacaa caccgtatcg ctatcggtga
    10321 cgacggggac tttcccattt ggattcagtt tgagaaattc aggcgttttt tgcgcatgcg
    10381 ctctcaggtc aacaggaact gtttcgtaag gcagattttt ttccgccaga acaacccgca
    10441 cccgttggca attaggtgac gacttgaaat cgtatagctt aatcattgtc tatcctttct
    10501 agatcaggct tgataccgca cttatactga gattccgttt aaacgcaaag gcgaagctga
    10561 aactcgcttg atatggctag tctttgatgt taagcgaata ggcgattagc cggagttttt
    10621 tcgatgagcg cggaagaagc agctgctaca aagatcgtta ccgggataaa acaagccggt
    10681 atcaatttga tcgccacttt gcccgacgtc aacctcgcgc agctgctgta cgctgtcgat
    10741 gaagaccccg atcttctcca cgtgccgctg tgtcgcgagg aggaaggaat cggtatctgc
    10801 gcaggggcct atcttgtcgg aaagaagtgt gccgcggtga tgcagaacgg tggcttcttc
    10861 aacagtaata acgccatcat cagcacgctc ttacagtatc aaatcccgct gctgctgctg
    10921 atctactatg ccggcgatgt cggtgaccgg accttcagca cttcaggcag catgacggaa
    10981 cccgttcttc aggcacttgg aatccgatat tacatcatgc gcgagaccgc cgacgcgccg
    11041 gagctcataa aacgggcgca gattttagcc gaggattcaa aaaggccggt ggcattgctc
    11101 cttaccaaag aagtcctggg gctccgcgcc gcgcttaagt cgctctagcc gtggttctga
    11161 catcaagcaa agctgaacgt ttcgtatgaa aagatttgat tgcttaaaac tggttgctga
    11221 ggaggcgaag gacgctttgg tagtcagttc tgccggagcc atgactctgg agtggaatgc
    11281 cctgcgtccg ggggacggca atttccgggt tcgcaccctg ggtctgtgtt cgtccatcgc
    11341 gcttggtatg gccttgggtt tgccccatcg caagatcata gccttagatg gagacggatc
    11401 tttgttaatg aatctgtgtt cattgccgac gatcgctcgc atgcgcccaa gcaacttagt
    11461 ccatattgtt ttcgataacg aagtttatga ggcctccggc agcaagaaaa cggcgactgc
    11521 ttgtgggact gacttagtcg gagtggcgcg cgccgcggga atcaagaact ccggttgggc
    11581 aaataatctc agcgagtttg gtagagcgat tttggcgggc atgactacca atgagctgag
    11641 atttattggc gccaaggtca caaccgagcg aactgaggtt agcccatatc cgctcgatga
    11701 agtggaaaac aagtaccgct tcattcgtca cgttgaaatg acggagcagg ttgagattct
    11761 caagaagaat ttgccggcca gttatgtttg actccgtccg agaccatctt tgatgcacaa
    11821 tgaaaattga tatcgaattt aactccgggg cacagctacc gatggatgca atcccggagc
    11881 tggcgcgtct cgccgaggca aatgggttcg attgtgcttg gggtggagag gctaacaaca
    11941 aagatcctac ggtaatgttg tcagcgatcg ccgcggtaac cacccggtta aaaataggtt
    12001 cggcgattta tcacatcctc ggccgcacgc cggcaacgtt agcgttgcaa gcggtgggtc
    12061 tcgaggagtt atccggcgga agatttctct tgggcatcgg ttcatccaat ccgactattg
    12121 cgaagtggca cggacaagtc ctagatcaac cgctcggccg ggttcgcgag tacatcaata
    12181 ttgtccgggc cgctatacgc ggggagaagc tcgacttcga tggcaacttt tttaccgccc
    12241 acaattttag aatggcattt aagccctcgg gacgcgctat accgatctat ctcgctgcct
    12301 tcggtcccaa gatgacacgc ttggcgggca ggattgccga tggggttgta attaatatgg
    12361 ccaaccccgc tcaaattcgc cggatatccg atgaggtccg aaaaggcgct gaagaggctg
    12421 ggaaaaatcc ggacgatctg cagattatct gtaaaattcg ctgctctatc gccggaagct
    12481 ctgcggctgc gcgagatgct ttgagccacg ctctgaccta ttatgcgctg gcggattatt
    12541 atcgcgacct cctcgggcgc atgggattcg cagcggaagt tgaggcaatg cgcagcgcat
    12601 ggaaagccgg aggttttcac tccgcgcgta agttggtgac tgaccgaatg tttgcggatt
    12661 tgccgctgat tgccgcgacg tctgcgcacg aaatactcga gcagatccgg ccctacgcgg
    12721 ccgccggtgc caccaggatt attctgccgt atgttgcggc gagtgatgag gttgtaggag
    12781 agatgaaggg tttcatcgag aagtggagtt cgattacgac tccggtcgat tgaggagaga
    12841 agctagatgc cttttgaatt gctgaacccg gccggtttag agtcgccagt cggttacgct
    12901 catatcgcca aaattaccag cggcacaatc gtccacgtag ctggacaggc gccgtttgac
    12961 tctcagggtc aggtcgtcgg caagggcgat ttcgtggcgc agttcgctca agtcgtccgc
    13021 aacctaaaaa ccgcaatcac tgcagctggc ggacacccgc cggattacgc tgtcctcacc
    13081 atctatatca cggacttaca agcctacctg aaaaataaaa agtcgcttgg gtcagtttac
    13141 caggagatat ttggtcgaca cttccccgca ataacactgg tggaagtgaa aagcctatac
    13201 aatgcggagt gtatggttga gatttcaggc ttggccgtta tcagttgaca gccagaaggg
    13261 cttttcctac agctcttgtc ttaaaattgc gcaattgtga caactttgca cttgccggct
    13321 taccttcgct aaccgtccca gagagggaag tcctggtatt ttcaccccat gtcggaatgg
    13381 catctccttt gcttcgatac ctaccgaggc aaataggaag ggtcaaaaca aatgagttac
    13441 cggctaacta gatacaaccc caaacctagc attgagcaag aacgggaaca ggatcttcta
    13501 gaaagcgtca ccgaggaaga ggtgatcatc ctgtggcgtt cgataaagca aaagggtttg
    13561 tcagaagacg aggcctggca tgcgatagtt accgggcttg ctctggatgc ggctttttca
    13621 aacaatcgcc gaacgatcca atatgaacag taggtgcgag ttactgttcc cgaagcgaag
    13681 cttcttctct gatgctcaag tttgagttgc ctgtggatct gcgagcgcga ttagatccga
    13741 cagaatccgg cgatgtgatc tctgggtacc cctaagctct tctaccaatt ttgcgatctc
    13801 tgttgtttct cctgccttta gttcgagcgt gaacagtaaa tcatccacgg caacctgatc
    13861 ctgcaagtcg aggtttagtc gctcccaatg gtttattcct gttttcgact cacccgtcgc
    13921 tgatcgcggc tgttctccaa gtttctcgag caggattctt agctttctag caccctcgta
    13981 tttttcgctg gccattctct gtaatgttcg catcatatga gggtaaggcg caccagcagc
    14041 atgagcctcg atttgagtgg ccagctgtaa aatgagctca tgggcgagga ctagaccttc
    14101 cacggctgtt tcatagtgaa gaccatttaa tttgctgagt cccggaaaga aaaaattctt
    14161 cactgatttg gcgattgtgc tcggcattga agatcgtcgc ctccgacgat aaatttaagt
    14221 tgcttttatg tcaagcagtt gggcttgtaa ctcgttgact gagggcgaaa aacctgattg
    14281 aatctcgaca gaaaattagg tgacctgtcc cagatcagcg cttgaatatg aacttggatt
    14341 ggactgccag ccagcttggc gctttttgga ttcaaacgag ctaaagagcg ccggaccact
    14401 taccagttgt gagaatggca ggatcgtggt ctctgcatga gcttgccgct cagatcgaaa
    14461 cgccggaaag gtcattagaa aataacaatt cggttatttc cagccgaact ggtgggctcg
    14521 cctcgcatag ctcattgaat cttcgatacc gcaaatgacc gtagacgaca aaacactacc
    14581 catcgattta ccatttctca acatcgagtt gtcaaagcaa gaggatttga aagacgctga
    14641 ttcgaaagaa aaagcgttgg ttcccttcga tccgttgcag cgatatctcg ccgagattcg
    14701 ccgttttccg ctgctttcac gcgaggaaga acataagctg gcggttgagt tcaaggagtt
    14761 tggcaatatc gaagccgctt ataagcttgt cacgggaaat ctgcgattgg tggtaatgat
    14821 cgcccgagaa tatcaaaagg cattgaaaag cctgctcgat ctgattcaag aaggcaacat
    14881 gggcttgatg gaagccgtaa agaacttcga cccttaccgc ggtgtgcgat tcccatcata
    14941 cgcagtgtgg tggattcgtg cctacatcat acgctacata atgaacgact ggcgtatggt
    15001 caaaatcgga acgactcagg ctcaacgaaa actttttttt aacttacaga aagagaagga
    15061 gcgaattgag gcagaaggtc tgacgcccgg tcctaaactg ctggcgcagc ggcttaatgt
    15121 taaagaagac gaagttgtgg agatggagca acggctagcg aatcgcgacc tttcggtaga
    15181 tatgccgatc ggtgaagacg aagaggctac catgctcaat tttctgcctg atcagaagca
    15241 aaacccggaa gagcatttcg ccgaaagtca gtaccgcgaa ttgttgcaac aaaaaatggc
    15301 agagtttgcg cgcaccttaa aagataaaga gcttgtcatt tttcgcgagc ggctgctgaa
    15361 tgaagacccg attacactcc gcgagatcgg cgaaaagtat aatattagcc gagaacgtgt
    15421 aaggcagatc gaagagcggc tcaaaaagaa gcttaagaca tatttgagca aagaattgga
    15481 tgatgtcaac gacggcaaaa ccgtcaaaac tgcagattaa tagccgttga cccgagcaga
    15541 tgccatcact ccgaaaggct cgcatttggg atcttgtctg ttcagctgag ttttggtacg
    15601 agaaatagat tcacttggga ggaaaccatt gcagttcgaa aaacaggttg agattcaggc
    15661 gcccagagaa aaggtttgga atttcatctg ggatgtcgat cagtttatcg cttgcgttcc
    15721 aggttgtaag gaagccaaga cggttgaaga agggaagcgg tattccgcaa ctatggtcga
    15781 gaaagttgga ccgttcaagg ttgagtttcc gacgaccata gaagttctcg aacgggacga
    15841 gttgtcccgt atcaaggctc aggcttctgg cgctgataac aaaatcggca gccgcatgaa
    15901 attggatctg gaagtgaagc ttcgtgaaga agacaacaaa accattctgc aatttgttgc
    15961 cggagtcgat atcttgggta aactcgcagc cttgggacac ggtattatca aacgcaaggc
    16021 cgaccaggtt ctggacgagt ttgcccaggc agtgaagaag cgtttggaag gagactcgtg
    16081 agatgctgcg acgattccgt ctagaagagc cgaccagcgt agctgaggtg tcggaactac
    16141 tggggagatt cggtgattcg gccaaaatct atgcgggagg aacggagctt ctgctcgcca
    16201 tgaaagaggg cctggttcaa tatgagcgac ttgtcaatat taaaggggtc gccggcctaa
    16261 acgaagttaa acaaaataat ggggaggttc atatcggtgc gctctgcaca catcgcgaac
    16321 ttgaaaactc cccgctagcg aaggaaaagt tgccttccct ggtaaagctc gaagaaaatg
    16381 tggcgaacgt ccgagtgcgt caagtaggca ctcttggtgg caatctttgc tttgccgaac
    16441 ctcatgcgga tcccgggact ttattgatcg ctttcggagc gcatctcgtt gccgagaaag
    16501 gagatggaaa gcgcgagata ccggcggaat ctttcttcgt cgatgcttat gaaaccgcgc
    16561 tcgaaccgga cgaggtgtta acgcgaatcg tcgttccggt accggcacaa ccctcgggaa
    16621 tttgctatat gaaatttggt tacctcgaaa ggcccagtgt gggagtagcg gtcgccgtaa
    16681 agcttaatag cggcaaaact gcgattgacg atatcagaat tgcggttggc tgtgccggac
    16741 cggcgcccaa gcgagttgcg gaagccgaag cgttcttgaa aggcaaaagc ctcgatgacg
    16801 cgacgcaaaa gattccggag gcgggggaga cggctgggcg ggtttcccag gcaatcagcg
    16861 atttacatgg cgcgcaagat tacaaggagc atattatcca agtacttttg aagcgctcat
    16921 ttcagaatgc ccttagccag tgcacttgaa ttcaggagag ttttagatgg caatgacaac
    16981 gttacgacgt tcggatttac agacgctacc catttcgttt accctcaacg gtaaattgca
    17041 ggaactggat gtcgagccgc atgaattgct gttggacgtc atacgggatc ggttggggct
    17101 caccggagcc aaacggtcgt gcgacgtgca agtttgcggc gcttgcacct tgttggtcga
    17161 cgggagaccg gtgagcgctt gtacaacctt agcctttgaa gtcagaggcc gaagtgtgat
    17221 gaccatcgaa ggtttggcgg aaaatggcaa attacatcca ctccagcaag cctttattga
    17281 acatggcgga tttcaatgcg gcttttgtac gccgggaatg attctggccg ccaaggcatt
    17341 attagatcaa aatcctcatc ccaccgaaga agaactaaag catttcatgc acggcaacat
    17401 ttgtcgttgt accgggtata aaaagatcat cgaatccatc atggccgccg ccaaaaatat
    17461 ctctgaggcc acccactagg ttcttcgttc tcagatgccc ttttggacca gatcaccggc
    17521 tagtttccgt tctgggctgg tttgttcacg tgtcggtctc gatcgaggaa gtacaaacgt
    17581 gtcggctcgc ttaacgagtt cttagctatc actcgcacga tcatgtgatt ggcttccacg
    17641 acctagccca gctcaagcat caaatctcca gtcacgactt gttgtcataa tccgcttcag
    17701 tttttgccag caagctgaat cagatcgaag atccaggcgt gccataacac ttccgtgagt
    17761 cgctagcagt aaattgatct tgcgggtggg gtcttctccc atggactatt ataggcgagt
    17821 ccatgagcaa atcgaagttg atgttttctt gagaacttga gaactcctat tgagctctct
    17881 acgcgtataa attttgtagc aggataatga ccggcctagg cacgaatttc agcgcgcatg
    17941 aagaagatgt aggaaattcc gaagcagacc gccaccaaac cgaatagagt cgtgacgtgg
    18001 ggccaaataa gggtcaaact ctgcccaagc gcaagcggcg tgggtaaaat accttccgcc
    18061 tggctttgta aggctaaccc gaaaacgcga gcagctggat tcagcaagat gtgcacggtt
    18121 tccgaaaata gagtgcttgg cgagatacga ccgatgatgt tctcgatgtc ggctcgtcga
    18181 gcgacggatt caggagttga agtatcggga acaacgatac tcgctatgcc ttgactgacg
    18241 atcactacga acagggtcat aaaaagccac acagcaattg aagccaaagc agcggttacc
    18301 gtcttctgga agagcaatga aaagagcatg gctagcgcca gccaaaaccc gatatagaaa
    18361 atacccacga cggcaaagat cagcatccgc cacagttcct caccattggg tggaaagcca
    18421 agcaaagtaa taccgagccc gattactagc aagagaatcg atgcccacaa aacagcaatt
    18481 gtcgtcagac cggcaaaaaa cttaccgttg atgaaactgt cgcggtagat tggctgtgag
    18541 agaactcggc tcaacgtgcc gcgggcgtac tcaccgctga tagagtcgaa tcccaaagta
    18601 atgccgacta acggcccgaa aaacccgaga aacgtcgcca acgaaaaaag cgtttcgccg
    18661 ctcgaggtta gcaggagaag gaaggcatat tgtgagggaa cggtttcagt gttctgcctg
    18721 attgcttggc cggttgcata agtcgcccaa agtccggtaa gtacgatgat aaaaatcaaa
    18781 atcatgaacc ggcggctgct aaagtgatct gccagctctt tccaaaagac cacactcatt
    18841 tttaagcctc gcgaaaatac ttcaaataga tttcttccag agtcggatct tcagatcgca
    18901 gctgtaacaa aggaagtcct ttggtcgtga ccagggaaac gacttccggc cgtacgtctt
    18961 tggtgcagcg taaaaaccag ccttggctgc gtttttcgat ttcgtcgatg ccgtcgatcg
    19021 attgcaggct tcggtcgagg ctgtcccgat tacccccgac ttcaaccaga aagttccact
    19081 gccgaccctt gagaatcgct cgcccgagtt catccatttc gccttgtacg atgaggcgac
    19141 ctttgacgat gataccaacc cgactacaga tttgctggac ttgctgcaac tggtgcgatg
    19201 aaagcatcac ggtcaagttg tgatctctat tgagattctt gatcagttcg agaatccgga
    19261 tcgccccatc cggatcgatt cccaaggttg gctcgtccaa aatgatcgct atcggatttt
    19321 taaccagaac ttcggcgatg ccgagcctct gtttcattcc acgagaaaac tcgcgaacca
    19381 agcgccggcc atcttcgctc aggccaacct gctccaggac ttccgcggtg cggcgttgcg
    19441 cttccttctc cggaatccga ttgaggcgcg ccatatagag aaggttttcc tgcgcgctaa
    19501 tatcgtcata gaagcctgga ttttccggca ggtagccgac tcgctttttg acctcgagag
    19561 gatcgtgcgt gggattatac ccgcacacgg acaattctcc tgaagtcggt tcagtcaacc
    19621 ccaacagcat caggattgtg gttgtcttac cggcaccgtt aggaccaagg aaaccgaaga
    19681 tctctccctg ttggaccgac agagtcagag tattaacggc aagcttttca cgataacgct
    19741 tggtcaaatt cttggtgtct acgactgcgc tcatcgccgt cctaaacgga aaaacacaat
    19801 caccaatcca agcactacca acgcaacgat tccgaatcct acccagcccc agagagtcgg
    19861 cgttgagacc gtcacccgaa actccacgga acgattcacc tcgggagagt ttgccgtcag
    19921 ggtcatcaag tagtcacccg caatcgttcg gtccggcgct aggatatcca tttttatctc
    19981 gcgcacttca ccagggttca aagcatcgat tttgtcgggt ctaaactcga ccgtccactt
    20041 atcgctgggc ttcttgctga tgaaattcag attgcgaatc ggcgccgtac cggcgttggc
    20101 aacgacaaaa tcaatcgggg tccggttgcc agcggtcact gaggtattga gcgtcccagc
    20161 caatgaaccc attttaagat cttgggttcc tttaagattc accttcaaat ctgcactggc
    20221 ttcgaacgct cctgatcgcg ccacaacctt taccggttgc tctttggcct cagcgttggc
    20281 aggcgtatcg atttcaacag caacggtttc ggaaccattc tctttcaaaa gaattgacga
    20341 gtactgagta tcgccgaatt gcggcttgaa acgaacggcc cagcccggcg gtgtctgcgc
    20401 ggtgagtgac accggcaacg gtttgttggt ttcattcttc agatctatcg tgaacttaag
    20461 ggtttgcccg gaaggcgcgg taagaaccgg atattgacta gtgagtctta agccgccggt
    20521 agcgacttta gcggaagtca cacggaagtt gattgtgtcc gagtatgaag tcggtccttc
    20581 cgtgtccttg gccacgacct tgatgtcgta attccccggc ttggtgtttt gaggaacttt
    20641 actcttgaat tcaatggtcg tcgatttttc tccttgcacc gtaacggagc gaaccggata
    20701 gctgggatat ctggaattga aattcgcttc ccaacccttg ggaatagact caagagagag
    20761 attaacctcg accggatttt tcgtgcgatt gacaacctcc gtgtccatgg tgacttcctg
    20821 cccctgtcca acagttagat ctttgtacgg gatgatcaga ttgaagctct cttttgacga
    20881 cggagcgtct ttctgtcccg tgctttcggc ggccttttcg gcggcgaccg ctgacgttcc
    20941 cagcgaagca gagcaaagta aaaaccacga gaacagcaca agcatcattc gcaggcgcac
    21001 acgtagacgc acgaagctca tatcgaaaac cccctaaggt gcgaaatttg gccataaata
    21061 accatattaa tccatagggt caagagcttg cccgctaaac ttaagctttt cccagctgcc
    21121 gtaatccttc atagagaaca atcgacaccg cattcgaaag attcaagctc cgaacacccg
    21181 caccagccat gggtatacgg taggaagatt ctggattgtg ctccaacaaa tcttgcggta
    21241 agcctcgcgt ttccggaccg aagactagat agtgctcctc ttggtagcgt gctgtggtat
    21301 aggatctcgt cgcccgttta gaaaagtaga gaaactgttt catcgatgct gccgcgagga
    21361 actcatccca agatttatgt acccgcagat ctacgaactc ccagtaatca agtccggcgc
    21421 gcttgagatg acggtcgtcg attttaaatc cgagtggttc tattagatgg agaggggtca
    21481 acgtagctgc gcacagccgg gctatgctgc cggtgttcgg tgggatctct ggctggaata
    21541 gaactacgtg catgctcgac tatctaaaac gaaagtaacc ggaccgtcat tgaccagcga
    21601 aaccttcatc cttgcttgga actggccggt ctcaactgag actccggcat cccggaaccg
    21661 cttgatgaaa tgatggtaca gtttctcggc ctgggccaat ggcgcagcat ccgtgaaaga
    21721 cgggcggtta ccttttctcg agccggcata aagagtgaat tgcgagacaa ccaaaacctc
    21781 gccgcggatc gccagtaaag atcgattcat cttgccttct tggtcttcga agatgcgaag
    21841 attcttgatc ttttctgaga ggaaatcggc atccggcaga tcgtcgtctc ggccgacacc
    21901 tagtagaatg cataggcccg atgctatctc gccgacgatg ttttcgccga ccgtgacctg
    21961 agctttgctg accctttgaa tgacggcgcg cacgctttcc cttgtcaacc caaaacgctt
    22021 atgccagtac tgaggcgggt tctagtaaag agtggaatca ccagacactg cgaagagtcg
    22081 taacagagaa ttcgcaaggt ctcaaccgga cggactcgtg gatccgattc gtacgagccg
    22141 atctcgctcg tctcacgcta gctcccgacg ttctcggttc caagagtacg aaagtcagat
    22201 gaaacagata aaaattttgt gcatgctgac atggcttgtg tttgcgctca agcccggcag
    22261 tggaactgcg gaatcgcctg actttcactt ccggcctgcg cagccaatcg ccatccacgg
    22321 gcaactccgg ggaagtgttt tgctctcggg aaaataccaa ccgccagatc cgctcaaagt
    22381 ggtaaagagc cgcagtttct gcggtttgag cgtgccgaac gaaacgttga ttgtggatca
    22441 gagcggtggg ttgcgcaacg cagtcgttat cctgcgcccg atcgatcgaa ctgtgaaggt
    22501 ttatccgagc aggattttat tggacaacaa gcaatgtgca ttcataccgc atgtgcaaat
    22561 cgccggcatt ggaagcgagt tactgctaaa aaacagtgat ccgattctcc ataccgtgca
    22621 tgcacggctc ggcaaggaga cgctttttaa tgtaggttta ccgcggtggc ggcaggtgtc
    22681 caagattctc gacagagtag gtgtcatcag aatagactgc gatgtgcttc atacctggat
    22741 gaccgcagct atcgtggtgg tttcaagccc gtatttcgcc gtcaccgatg agaccggttt
    22801 ttttaacatc caagaaatcc cagcaggaaa gtacgagctc gagatctggc atgaggtcct
    22861 gggcagtaag accgcgcaaa tttctatccc ccaaagcggc actattctgc tcgacgctat
    22921 ctataatcga gtacccgaaa taaagtcata ggttcttgct atcttgatag tcgcgatctc
    22981 tccggtatga tcggtttcca tgtatcctat tatcttccag cttgggccaa taacgattta
    23041 tagttttggt gtcttcatgg cgctggccgc gctatccgcc gcatgggtcg ttcactcgga
    23101 gctaaaaagg cgcggctatg atcccgaact ggcatcttca atgatctttg ccgccgccgt
    23161 tggcgggctc atcggcgctc ggctgttgtt catcctgggc gaatggcatg attttttaaa
    23221 ttctccctca agctacattt ttaccggtgc aggatttacc tggtacgggg ggttgttcgg
    23281 cggcgcggtg gcggtgtcct ggatcgtcag gaagaatgag atcccatggc tcgtcggcgc
    23341 ggatattgcc gctccggcgc tggctcttgc ctatggcgtg ggccgcctcg gttgccattt
    23401 tgccggagac ggagactggg gttcgatcac gaaggcccct tggggcgttg cgtacacgaa
    23461 cgcgattatt ggttgggttg acccgatgac cggcattcct tatccagccg gtgttcgcgt
    23521 gcatccaacg cccatctacg agtttctcca atcaatcgtt atatttgcga tcctctggag
    23581 tatcaaaacc cgggggcttg cccctgggac gctcgcgtgg ctgtatctgg tcttggcggg
    23641 tcttgcgcga tttacaattg agttctggcg cgtcaatccg atcttggcgg ccggtctgag
    23701 cgaggctcag tggatcagcc tgattctgat agcggtgggg ttggccatgt tagtatcgcg
    23761 ccgggtccgg ctcgcgtatt gaaagtgtca gatggtgtta aagagtgagc tctggaatcg
    23821 gaactgcacg ggctttgtct gttgctgttc tgcttctttc atgaaattct ttcctaccaa
    23881 ctttccgaca ttacctggca taccgctcga taaatagctg caacgtcctt gccggactcg
    23941 atcaggtgct gtttgcaaac atggctgcac aatgcaatcg cagcctgaaa gatgatcatc
    24001 gccttgccat ttttagtcag gtggccgctc agtctgccgt gacgtagaaa aaccgtttct
    24061 tccggatgat agatcaaatc gtaaaaacga acgtttggtg ctattgctcg cgctaaagtc
    24121 atggaggctt ggttgttttc atcgatatcg gcccgagctg cgcacaccca cttctgccgg
    24181 gtttgcgagc cggcgaaatc tgattcagtg atcgataccg ggtgagcgga tgctaaagcg
    24241 gagtagggtt ccaggctggt aagctcgccg ttcggtagtt tacgaatccc tccttgccct
    24301 ttggtggttg tgttaacgat taagccgacc ctacgagccc aagggccgat ttcagtttcg
    24361 tcgatagctt gagctttccc gccgtttttt tgaagttccg ctgataggga aagagcatgt
    24421 tctaggtttc ggttggcgat caccagctgg cccggagcga gcatatccga cagccgaaag
    24481 gcgatggcgc gcgccgaacc gccggcccct atgagcaaga cctcggttcc cgccagcgaa
    24541 gtcaggaatg attcagtctc ctctggcagc ggcgttagca aactttcaac gaatcctgaa
    24601 ccatcggtat tatatccgac gagtttgccg gacgagctct taacgatggt gttgaccgct
    24661 tgaactcgtt tcgcccctgc ctcaatctcg tctaaaaagt ccatgactcg gatcttgtgc
    24721 ggcacggtga cattgattcc taggaagtta tctagccctt tgagaatagc gattagctcc
    24781 cctaagcgtg gctcagcgac atcgaagggc agataaactg cgttgatgcc cagataattg
    24841 aatgcggcat tccatagggc agggcttctt gagtaggaag acggcgcgtc gccgataatc
    24901 ccagctattt gcttattatg aattaggctg gcgtcgatcg cattaccgat gcaattttga
    24961 atggcagcga tttgtttccg agatgtcatg gcagatagct catcgcccca tagtcgcact
    25021 cagggatgct tccgcaggaa gggaagttgt tgaatcgcgc tatatgccgt ttttgattgc
    25081 cagtctgtta tcggagtagc acagcagcga cttgcggcta tcgagaaccg tttccaaagc
    25141 gacttcgtgt ccccacagct ggtttagata ttgaagcgtt ttctcggcat actcgagttg
    25201 cagatcccgt ccatcatgat ggtgcctgag gaacagcgtc cgattcttgt tataatcggc
    25261 atcttcgatt tttatcattg gaatggtgct ggtgccgaca ttttgaatca aagtttcttt
    25321 gatctgacgc cagttaagat catccgcgac gcggctgacg acctgctcat tgccgcgggt
    25381 tcggtactcg aacaggttga ggtctcgcac gagctcttcg gtgaggtagc gccgtataaa
    25441 ggaggcatca cgttcaactt cccgcacctc gaaaagtttc tccgcgccgc ttttgtttcg
    25501 gcgtccgtac tctttctcat cctcagcaga ggggtggtcc cagcgttttt cgatattctc
    25561 ccaaatcttc atgcccacgt ggtacggatt taacccgccg ggggcagggc gcaagacctg
    25621 cgcatgccgc accagaaact ccagatgcag gttctgcggc aactccaagg tttcaagaat
    25681 tcgtttgtgc caaaagctcg cccaaccttc attcattatt ttggtttcaa tttgcggaat
    25741 aaaatactga gattgctcat gcacgatagt taaaagatcc ttttcccaat cagccaaatg
    25801 cggattgtgg tcgcggatga agatcaataa atcttcttcc ggataaagag gaactttgtt
    25861 gagatccgga gacacataat cctgccgtct atgaatcgcg ccaaatgggt ccgcaggcgg
    25921 ttgcgcgtcc tgcactttag tttctatttg ctcctgggag gacagcttgc gaatcgacag
    25981 gttgcggcga cactggagcg acaatgcgtg tgccgcatca agaatgcgtt cgatgcgttc
    26041 caatccgata ctcggatcct ccacgtagtg gcgcacgcga tttgcatggc ttttgaacgt
    26101 ttcaatcgta tatttcgcgc gtgtggcgcc aaatgtgaaa ttgtttttga aaaaatcgtt
    26161 atggccgtat acgtgggcaa tggtgaggac ctgcaaagcc aggctattat cgcgcatcaa
    26221 ataagcaatc gagggattgg agttaataac catttcataa ggcaggccgc tcaccccgta
    26281 ctcgtataac gtctttaact tttcgaagct ttttccgtaa gaccaatgag ggtagtgaga
    26341 gggcatgccg gaataaacca tgtaagtaag catgtcctca tgatcgcaga tctcgaactc
    26401 ttgaggatag gggtcgagac caaactgatg aacaatctca aggattcggc tgttccaagc
    26461 ttccaaatct tcaatcgtat aatccatatc ctgaaactac ctatcgagga cgctaagcgg
    26521 catcctcccg accgcgatcc ttagccagga aagtcttaaa cgatgtccag atatcctcct
    26581 tgcgatgaat ctgaagggtg tgaaagttcg gctcatccaa ttgtgaaaat agcggaatca
    26641 agctgctgcc atagtagccc gctcccaatg gtttgatctc accgtagccg aacaggttag
    26701 acgatcgtgc caattccgtg gctgccttca aagccgccgg attgtccgat tcgaaattgt
    26761 cgccgtccga acaatgaaaa acgtacacgt tccagagcgc gggatgaaag cgctcgttga
    26821 taatctccaa ggccttattg tatccggaag aaatgaaggt gccgccggat tcgcccttgt
    26881 gaaagaaatc ttcctcggta acttcctgcg cctcggtgtg atgcgcaata aaaacgatct
    26941 caacattttg atagcgcgtg ctaatgaatt gatagagaat aaagaagaaa ctccgggcca
    27001 ggtacttctt catagtgtcc atggaaccgg acgtatccat gatgcacaaa acaacggcgt
    27061 tagactcccg tctcaagtcg ttgactgaat gatgataacg caggtcgtct ttatggaacg
    27121 gaaaccggct gcgcgcgccc atcagatctc tgcgttgaga ggctttctta cgtttcaaac
    27181 gcgagcgagc ggtccgcttt ttatccaaac gcacgcgaac gcctttgcgt ctgtagcctt
    27241 tttgacgaaa ctgcttctcg acttcaattt ggcgcagccg tttgcgctcc aagtcgggca
    27301 gctccagatc ctcgaacatc aattcgatta gctcatcgag ccgaacgtcg gtctcatagt
    27361 aatcgacgcc gggctgatct ccaggctgcc ccggctgttt tcctttctcg ccttggccga
    27421 caacttgtcc cggctgcggc tcttgatccc ctccctggcc gacgccgggt gcgttatcac
    27481 catacacaaa ccggtattcc ttaactccgc gaatcgggac tttgataatg cggttcttgt
    27541 ctttaccgat gatggattct tcggcaatga tgtcggctat gttatttcta atcgcttccc
    27601 ttactttctg ccggtgccgc aggcgatcac ccgcgctccg atcactacgc tggcccgatg
    27661 acgagttgta cggacggaag atggtctcag ccgtgcgatc gatcccggtc tgcgggtcac
    27721 gcatgaccgt catgatttca gtccttccag agattattgg cggcatactt gagaatgaca
    27781 tcgacacagc tcgggcaata gccgttttcc agcaggtttt taaccataac gttgtacttt
    27841 tcggattgtt cctcgtcacg catacgggat ttggtgatga cccggctgag atcccggacc
    27901 gaagtgatga gcttcttctc gatcgcttcc tttaagggtt catagctttt gtaatcgatc
    27961 ttttcaccgc gccggtttgc cgcccaaaga tatgcgatga cttcttgacg gaaaccgtcg
    28021 gccgccgagc cgatgatcgc gatctgttcc tcgatagact tcatgaagct ttcgtcgggc
    28081 gagagctctt ccttggtgtt tcggtctttg accttgcttt tgttgacata agcttcagcg
    28141 tgatccaggt aattttggaa gagcgcttca gcttgttcct ggtaggaata cacgaacgcg
    28201 cgagtgatct ccttctctag gacctctaga tattccttgt gcagcgcatc ctgcaacaat
    28261 tctaggtatt gcttcctggt gtcgtcgggg agatcggctt ccttgaccat attgatcaat
    28321 gcctcgcgca cgttgatcgg attgatgcaa ttcccggtcg tgttatccga taaggcgttg
    28381 tccaaagctt tcatgataaa tcgcgttgaa atgccggaca gaccttctcg cttgacgcct
    28441 tccttgagct cctgtacgtc gacctttttg cttctgcctt tttcgacgac ctcttcacca
    28501 ttgtagaggc gcaacttcgt catcagatcg catttgttcg tcggctcgag ccgcgaaaga
    28561 atggcaaaca tcgaggcgat ttccaatgtg tgcggcgcga catgggcgcg gaagtccgag
    28621 tggcggataa ttttttggta aatcttaact tcctcagaga ggcgcaaatt atacgggaca
    28681 cggataacca cgatccggtc tagaatcgct tcattggtgt ggtcggcctt gaacttctgc
    28741 cattcagcct cgttcgaatg agcgataata acggtatcca cgtacactag cccatgccgt
    28801 ccgggagctg gcacaacttt ctcctgggtt gcggtgatca tgcagtggag atattcaatc
    28861 tcattcttaa agacttctat aaactcgacc atcccgcgat taccaacgtt caaagcgcca
    28921 ttgagctcga gaacccgcgg gtctccttca gaatataagt cgagcttgga gatgtcctcg
    28981 ctgccgatga gcacggaagt gtcctgattg tttggatcga ccggcgggac aacgccaaga
    29041 ccaacacgcg cgcgcttgga aaactcgata gtcgagaccg ggaattcttc gtactttccg
    29101 ccatattcgt gctgtagttt gaaccgacag accgggcaga ggtcgccttc gatgtgcact
    29161 cccagcatct tttcgaactc tttgcgcagg tggcggggaa tcaagtgaag aggttcttcg
    29221 aacatcgggc agccctcgat ggcgtaaaag ggtgggcttt cttccagtcc ccgatgaagt
    29281 ttttcgacca gcgagctctt ccccgaaccc acgggtccca tcaggtagag aacttgacgg
    29341 ctttcttctc ccttcaacga agccgcgtga aaatagcgaa caatctgtga gatggtcttt
    29401 tcaatgccga agaactcatc ggcgaagaag tcataaactt ttatagattc gtctttgtaa
    29461 atgcgtttgg cgtgtggatc gtcgtctacg ttaatatctc tgacgcccgc ttttatgagt
    29521 acgtcataga gacgcgcgtg agccaacttc gcgactcccg gatcatctct gaccttgtcg
    29581 agatacccga gcagatttcc gcgccatgat tggctttggt gcagatctcg atctttctga
    29641 atgagttctt ggaattcctt gttatggtcc atgccagaag ctcccctttc tggagaaaag
    29701 tttatcgcgc aggttcagcg acgacgttgg tagagataag cggccgagat aaatgaggga
    29761 tgaataagac ctgtagccag gctgcgctat aggcgcagag agcaaaaatg atttttccca
    29821 aacgtgcttc acgttaggcc gaaagctggg aaggggtgag cggtgtggcg ctgcggttgg
    29881 taaagcaaga atcgggggaa gtttatggct tgcattcgac ctccgttaac ccaacgtgct
    29941 ggtcttctca acccatgctc cttgattaga cgtggccaaa ggacagtttc ttcattctta
    30001 cccgctgctt acagtcgaca ttgaagcgcc gtttgtgcgc aatgtcaaga ttattatggg
    30061 catgctggca tcatagcggg tgtcgaatac gctcagttat catctgacat tcgatctgct
    30121 aagttcgtgc tgactaaaga gcggtgaaag aggcgactgg tatgaaacct ggagaactga
    30181 tgattcaacc cggcctccga tggtatgcaa tccaaactaa aggtaacaag gaaagagagg
    30241 tagccaagcg actgacggat cttaagtttg aaatctttct gccttggttg cgcgatcgga
    30301 ggcgtatcgg tagcaagtat cattgggtta tggtgccgtt gtttccaggt tatttattct
    30361 gccgtttgga tatagttgta tcagggaaga cggcgcgcta ctcgccgggc gtaaaggact
    30421 ttctccgatt cggtaatgaa ataaccgaaa tctccaacga catcatggtc gcattacagg
    30481 agcgttgccc cgagggcgtc gccgccgtca acccggtgtc gctgaccgct ggcgactcgg
    30541 ttaccatcaa cgaaggaccc tttgccggtc tgcaggctat ctttgaaaag aaaatgaagg
    30601 gcagcgagcg cgttgccgtc ttacttgagc ttctcggtcg gcaaacacgg ctcattctgc
    30661 ccagcgaaac tgtcaacaga acttgacctt tcagttcccg acctgccctt atatatccca
    30721 acgtttcagg ccgacgccag actattcgcc gtcgcaaccc tcgctacctt gacatgccct
    30781 gagtgctgtt gataaatgcc aggcgctcaa aagtcatact tcaggggagg acgacatgcg
    30841 ccatcaagct tggcaccggc gagggctgtt ggttatcttt gctgcttttg catttgttat
    30901 cgtctgcgga acagtgctgt catctgtcca gtggatctcc aaaccctttc ccgggttctt
    30961 cgtgcatgag aatctcactg tcggcccgta ctccttgcct cactggagcg gcggtgttgc
    31021 aggactccag tctctagatc ttatcatcaa ggtcgacggt cgcccggtaa gcgggcgcgc
    31081 cgagttctac gaacgcctgc gcaatctttc ggtaggaact cgcttaactt atacaatcgg
    31141 ccggcgttca aatatcgtcg aattgaccat tcccagcatg atgctcacct ggcaagactg
    31201 gttcttgagt tttggagttt acgtttttac cggattggca ttcttcgcaa tcggcgtggc
    31261 gccttattgt tttcgagcca actcgccggc agctctgccc ctgtctttca tggtgatcgc
    31321 tgtgttcgtt tggtttgaga gcaccttcga ttttatgacc gatgggtttg ttcctaaaga
    31381 gatgcgccta tttgctatga ttctcactcc cagcgccggc attcatttgg ccttgctact
    31441 gaaacaagga cggcctgtac gacacacgaa gccttttctc ctggtattca tctatggaac
    31501 ggccctagct ctcggcgctt tgagcagtat tacgttctac ggaccggtgg atcgatggat
    31561 cgacatattc cgaataaatt atctctacgc ctgcatcgga gctttggcct tcttatcgat
    31621 tatcggctcg gctttgagga agacccccac ggctctcgag cgctcgcgtt tgcgggtcat
    31681 gtttgtcggc gcggttctcg gatttttatt gcccactgtt gccacggttt taaccagcac
    31741 gttccaatgg gcgatacctt ataacctggc aatgatctcg acggtgtttt ttcctctttc
    31801 ggttgcctac gcgcttttga agtacagcct cttcgatctg ggtaatacgc tcaagacagg
    31861 acttacccga ttatcattga tggtgttcct cttagccctc tatgcggcta ttttctttct
    31921 cgtcggacct tcggtcggcg tctacgacaa ggatcccctg acgccgctat ttttctcgat
    31981 gcttgtggta ttgatcttca atcccgtgct acgcggtata gaaacattgg tcgatcgtta
    32041 catctatcga caagagtatg atccggtaag ggcccagcgc gatatcagtc tatttctgag
    32101 gtcgctgaca accgctcggg taatcgccaa tgggttctta gagcaggtta catcgattct
    32161 cggcgtaaag aaggccgtct tgatttaccg ttctgctgcc gtgccaaacg atctgaccgc
    32221 tgcaaccgac gggaacgaag taaagtttgc cccgcttctt gatcgcgcac gacttttgtg
    32281 gagagctgag cacgtcgcgg gattcagagg gttgtcacgg agcgaggttc tcgcaaatcc
    32341 cgttttcgaa tccgagcgca gagagtggtt ggccatgttc gagccgctcg cttctgaact
    32401 gttgataccg attgtctttg aacatgaggt gcgcggctta gtttcttatt cggcgaagag
    32461 aggggggagg gagtatcgcg gcgatgactt gaccctgctg ggcactctga ctgagcagtt
    32521 ggccctgtca ctggaaaacg gcataaggta tgaacagtcg gagaaagcca aagacgagta
    32581 ccaccggctt tatgacgaag cggagcgcgc caaacggaag ttgatcgacg aggatcgcat
    32641 taagaaaaat ttcgtggcca acgtctgtca tgaactgcga acgccggtca gtaccctgat
    32701 aggctttagc gaaatcctcc tcaaccgcga acttccggcc gacacacgcg atatcttaaa
    32761 gacgatgttg agcaatggac aagatctatc caatatgatg gatggcctac tggatttttc
    32821 gcgcatggag gccgacgctg tgccgaacag tttcgagcgc atcaacgtaa aagatctatt
    32881 gggcgggctg gagctcatga cgcagagact tatccgcggc cgtcctattc agttcagaat
    32941 gaacatcgaa gacgccatcc aaaccatcaa cagcgatcca agaaaacttc agcagatact
    33001 agtccagcta ttaacaaacg cgcttaagtt cactgaaaaa ggggcggttg aagttaaaat
    33061 agcgaccatg accgatcaaa gcggcgggtt cttggagatt gctgtctcgg ataccggaat
    33121 cggtatcgat aagaacgatc aggaaatcat cttcgacagc tttcgccagc tcgatgggtc
    33181 ttctaagcgc cgtttcggcg gtaccggcgt cggcttaagc ctgtgcagca agcttgctgc
    33241 ggcgctgggc ggtaagatta gtgttaagag cgaactcggt ttgggaagtg tcttcagtgt
    33301 atttctgccc cttacaagtg tcgtgggaac ctcgcagcat gcagggtcga aggcttgatg
    33361 agcgaacagt gaagctctga gattgactca tcagatggcg ttagctagct atatctatga
    33421 tgacaagtaa agattttctc aaagaattgc tggcgatggt gaatgatttc tgccgaactc
    33481 tcgatgcgca gccgccagct tacagctata ttccactccg caccgatgaa gaacgcattc
    33541 gggtaatgaa gagccgtctc tataacgaag tgcgtgctgc cgagctattt ggaacgtggc
    33601 tcaagacaac gccggagctg gaagtcaagg cgataatggc ggaatcctcg catgaggaaa
    33661 tggagcacgc gcaactgctc tcaaagagaa tcgctaattt cggtttcgaa ccgtttgatt
    33721 atctaccgct tcctgcgcag atggcgatgt ttaatgcgat cgagggactc acggacacct
    33781 gtcaacggat ggcgggattc tcactagccg gagaagccgt agcaacttat ttgacacaga
    33841 agagtttaca agcatcgaca gtgcccgact ggattaaaca gccctaccga aaaattacgc
    33901 aggatgaaga agggcatggc agcatacccc aagagatttt gcagcgttac gcgaccactg
    33961 aagagcgtca ggagagagcc cgcagggcgg tggccatgcg cctcgtgcta tttcgtgagt
    34021 acttgaacag cctggaccgt tgggctatgg gcaaggatcg ttggtaactc gtctatctct
    34081 cactttgtac ggcttcgcag cgcggacaga tgagcggccc gggcccaagt aaaattgccc
    34141 agccacactc cgcgcaggtt gtcaccggtt ctcccaagag tccgcgaacc gcggcgccgc
    34201 agcgcttcgc tccgcaagga ccggtgccaa tgccggtgcg cttgctgacc atctcgaagg
    34261 tcttggcgcc ggcttgaatg gctttcacca cgatgccttt acgtatctta ttgcagaggc
    34321 aaatggggcg aaggttatct atgacagcct gcttcctggc ctcgttatca agttcgttga
    34381 tagcgcatcc ctttcaactc cgtagtctaa cacgatttct taatgctgtc ctccttggag
    34441 gcaatgatca gagatgagtt cagcggcggc ggcaaatcta tctgctttat atgcatcaga
    34501 ccggctgaca acatccagct tttaacttca tcgaatgcat agcaacgtcc cgccacggtg
    34561 gttaatagca tgagtaaact aaacacggcg ccgaccgttg ggttgacgcg cgtgctatcg
    34621 agaatatgat ccttgacgat aacgctgccg cccggcttga gcacagcgct aagtttgacc
    34681 atgagccgtt ggtttacttc aaagctttcg ccgtgaacga tgttcgaaag caaaatcgca
    34741 tcatagctgc cgggaatttc gtcaactcgg tagtcgccgc tgatcagtcg cattcgactg
    34801 cctaaaccgg ccttttggac gtatgatcca gttagtgcaa gagtggccgg caaatcgaag
    34861 atcgtcgccc gcagattcga aaatcgcttg caaagcgcga tgggataggt ggctggtccg
    34921 gaaccgacat cgagaagctc gcgagcgttg ccccaatcga aggctccggc catgatctcc
    34981 gcgtcgcccc gcgccttgac aagagtgtcc atagcatcga tgaagatcgc ggtttcctta
    35041 ggatcatctt gatacatatc cgcgggccga acaggccggc caccgcgaat agcctcggtt
    35101 aatgacatcc aacaatgcca gagcgaggct tcgaaacgga tcatgccgcc gaggtagccg
    35161 ggcgcgtctc gcttgagata ctttgccgat acatcggtga gagaaaatc
//
LOCUS       DASUVS010000134         4558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1142124, whole genome shotgun sequence.
ACCESSION   DASUVS010000134 DASUVS010000000
VERSION     DASUVS010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142124"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VF182_07730"
     CDS             complement(<1..767)
                     /locus_tag="VF182_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX6437006.1"
                     /translation="MAGEQVANSFPGLPQPRQPCSLGPFLTPQFLSRPWRGRNLVTGT
                     IDQGISPKGLDLQVVISPFQALTGGVLMLTVPSCTPCSRCGGAGKQGRFPCALCDGEG
                     LLRQTEEVRLGVPANAGDVTQIKVPLRGLGPHNFYLSVRVRIAWSIEGSKNFRRRFIG
                     EHMPKAIGIDLGTTNSVVAVMEGGKPTVIINNEGNRVTPSVVAYTKAGERLVGQIAKR
                     QGVLNPENTIYSAKRFIGRRYSEVQAEIKNVPYKLVSG"
     gene            complement(1066..1491)
                     /locus_tag="VF182_07735"
     CDS             complement(1066..1491)
                     /locus_tag="VF182_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX6437007.1"
                     /translation="MAEKTVAATGNPMPNQVGQGKERTRGMERFVPPPVDIYESPEGL
                     VLLADLPGVASEELKVRLEDNTLTIQASPKHLADGEATYKEFELVNFFRQFELSEEVD
                     QEKISASLSHGVLTLRLPRAEKAKPREIPVQVQGDRSRV"
     gene            complement(1528..1989)
                     /locus_tag="VF182_07740"
     CDS             complement(1528..1989)
                     /locus_tag="VF182_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX6437008.1"
                     /translation="MATWQSFDPLQALQREIENAFERAGPQFSPTFRSAFLPGRAARE
                     YPLINLYEEQDQVRVEALAPGAEPNSFNISVVGRTLTIAGEKPRVSGDVKPEAFHREE
                     RAAGRFNRNIEIPFAVEQGEGLTGQYRNGLLVVTLPKSEKAKPRQIKVQVA"
     gene            complement(2904..3491)
                     /locus_tag="VF182_07745"
                     /pseudo
     CDS             complement(2904..3491)
                     /locus_tag="VF182_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947702.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
     gene            complement(3537..4076)
                     /gene="ppa"
                     /locus_tag="VF182_07750"
     CDS             complement(3537..4076)
                     /gene="ppa"
                     /locus_tag="VF182_07750"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX6437009.1"
                     /translation="MDISKITTGRNPPIDLHAVIEIPVGGVPVKYELDKASGAMFVDR
                     FLHTAMFYPGNYGFIPHTLSEDGDPCDILVVGNVPVVPGAVIRCRPVGALLMQDEAGL
                     DEKVLAVPVDDLHPFYSNIRSYRDLPSMLIEQIAHFFEHYKDLEKGKWVKVVRWIDAP
                     EAEALIRAAIERCKAKQEP"
     gene            complement(4231..>4558)
                     /locus_tag="VF182_07755"
     CDS             complement(4231..>4558)
                     /locus_tag="VF182_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6717 family protein"
                     /protein_id="HEX6437010.1"
                     /translation="VFDDPAVGLRQEPFVSGADAIIERLVQQIPNAVSGFTLLFSAQP
                     FPGYQAEFDWRREEFSGNWYYCPELEMEGWLCPALLKYFDAAPKKIYAQFRPKAPNGL
                     ELDPPS"
BASE COUNT         1043 a         1234 c         1224 g         1057 t
ORIGIN      
        1 ccggagacaa gcttgtacgg cacatttttg atttccgctt gaacctccga atagcgcctg
       61 ccgatgaaac gctttgccga atagatggtg ttttccgggt tgagtacacc ctggcgcttg
      121 gcaatctggc ccaccagccg ctcgccagct ttagtatagg cgacgaccga gggggtcacc
      181 cggttacctt cgttgttgat gatcaccgta ggctttccgc cttccatcac tgcgaccaca
      241 gagttggtcg ttcctaggtc tataccgatt gctttaggca tatgctctcc gatgaaccga
      301 cgtcggaaat ttttgcttcc ttcgatcgac caggcgattc ggacgcggac ggagagataa
      361 aaattgtgcg gaccgaggcc gcgtagagga actttgattt gcgtaacatc gccggcgttg
      421 gccggcacac ctaagcgcac ctcctcggtc tgccttagca agccctcgcc gtcgcagagt
      481 gcgcacggaa agcggccttg tttgccggcg ccgccgcacc ggctgcacgg cgtacagctt
      541 ggaaccgtta acatcaacac cccgcccgtc aatgcctgga aaggcgagat gacaacctgg
      601 aggtcaaggc ctttcggaga tatcccttga tcgatggtac cggtgactaa attccgcccg
      661 cgccaaggcc ggctcaaaaa ttgaggtgtc aaaaatggcc cgagcgagca gggctggcga
      721 ggttgcggta atccgggaaa gctattggca acctgttcgc cagccattgc atcggcgttc
      781 ccggtgcttt gaaccgggga cgggatacgg tcacgttcgc gccgctcggg atttgatagc
      841 gcgtgataac accggaccag ctcttggaaa aactgcaagc ggtcgcagcc gaggctttcg
      901 ggatcataca gtttaacctg ctgtcgaaaa ctcgacggat cgtcgccgca gtttcaccgg
      961 gcgaaatacc gagcctgaag taatactcat tcataccgtc gactcattgg acctcagaca
     1021 acgaatcttc gtgaaaggga catgccacta tcctaataca gtagcttaaa cccgtgatct
     1081 gtcaccctgg acctgcacgg gaatttcacg cggtttggcc ttttcagctc gcggcaggcg
     1141 cagtgtcaac acaccgtgac tgagcgatgc cgagattttt tcctggtcca cttcttcgct
     1201 gagttcaaat tgccggaaaa agtttaccag ctcgaactct ttgtaggtgg cttcgccatc
     1261 cgccagatgt tttgggctgg cctgaatggt cagcgtgttg tcttcaagcc ggacctttaa
     1321 ctcctctgac gccacgccgg gcaagtcggc gagcaacacc agtccttccg ggctttcgta
     1381 gatatcaact ggcggaggca cgaatcgctc catgccgcgc gtccgctctt tgccttgccc
     1441 aacttggttg ggcattggat taccagttgc cgcgacagtt ttctctgcca tgattctctc
     1501 ctctcgagat attttttagt gagcaggtca ggccacttga actttaatct gccgtggctt
     1561 ggctttttcc gatttcggca aggtcaccac caataggccg tttcgatact ggccggtgag
     1621 accttcgccc tgttcgacag cgaatggaat ttcaatgttc cgattgaatc tgccggccgc
     1681 ccgctcctcg cgatgaaacg cttccggttt gacatcgccg gatacccgcg gtttttctcc
     1741 cgcgatcgta agcgtccttc caaccacact aatgttgaaa gaatttggtt ctgcgccagg
     1801 agctagcgct tctacacgca cctgatcctg ttcctcgtat aaattgatca gcggatattc
     1861 acgggccgcc cgtcccggta agaacgcgct gcggaacgtc ggcgagaact gcggcccagc
     1921 tcgctcaaag gcattttcga tttctctttg taaagcctgc aacggatcaa atgattgcca
     1981 tgtcgccata tgcatctcct ccctttcttt tattacggat cgaagctcat cgcccgagta
     2041 actgaggctg gcgagcgcac ggaaagtgtc ctaacacgcg gttattcact cgtgagccac
     2101 cggcccgtgt tcgtcagcta gatcattgtt gcgagaagtc agttcgggtt gattttgtcg
     2161 ccgtactgaa acagagccga ggcattcaac cagccgtact ctcgcggcgc aactgtcgtg
     2221 ccatcaagtc gcgttaggtg tatacagtca aatgttttta ccgggtaaaa agtctgacaa
     2281 atgagttggt gtttaggcga tgtttgctga atggtcgtgg gtagcgaggc cagctggaaa
     2341 atagccttta ggattcggac cgttgaagag ccctcactcg gtagcttttc gagcgcggcg
     2401 gctgcgtaac aagagttaat ctcaatcaag ggtaaattcg ttgctgggcc tgaatattcg
     2461 ctaatcattt ttagcattgt tcattgtttg gctgtcgcat aacagggacg cagccgctaa
     2521 agttaactac gcgtgtgatc gtctcgagat agtcgcacag gagaactctc tgattgccgc
     2581 cgagcgaatt gtgcagtcgt ttaaatgcac tactctgggg cgtttcaatc atggttcatc
     2641 accctgataa aattgctaag catcgtaaaa agggctgtca agcacccgct ctctgagtga
     2701 ccaaaactgc aggcccagat caggtgaggt taagctgaaa ttaacttctc gaaagctttc
     2761 gacacaatgg tcgaggttgg aggccgatac agctctgcag tgaccgcatg attcgcacgg
     2821 tgacatctcg acgcggaaaa taaaaaaggc cccctcgtgg ggacctttgt agccttcggg
     2881 agaggcgggt caaatccgac gcttcaagcc tcggcttcag cgtgttctgt tctagttgct
     2941 ccccagacct gccgggccgc atcggcattc ataataccga tgcccacgcc gacgatcaga
     3001 tcgggccagg cggataaggc gtacgcggtg acaaatcctg ctgtaatgat cgccgcgtca
     3061 gcaagtgcat cgtttcgggc tgacagaaat gccgcacgaa tgaggcttct ccgatcactc
     3121 cggtatcgcg caagcatgac ggcgcagacg aaattgatag ccagcgccgc ggcaccagcg
     3181 agagaaaggg gcagtggatc gggtggcaca ggcatgttga acttttgcca agccatccac
     3241 atagtcgcca gtccgggcac aagcagaata ccggcaagca acatacggac acggcatcgg
     3301 actccatttg agcgcagcta aaattaggaa attaacggaa gcgtcttcga ggagtctata
     3361 ctgtctgcga agagtgacac gaacctaatc gcgatggcga tagcaaactc cacaccgaaa
     3421 tatgccaggt taagaaatgc tacgagcctg acggtatacc gaaatgctgt gctggatgtt
     3481 ccgctattca tagctgatgc tccaactcga tcgtcattgt aggtagccag tacggatcat
     3541 ggttcctgct tggctttaca gcgttcgatc gcggctcgaa tcagcgcttc cgcctcaggc
     3601 gcatcgatcc atcgtacaac cttgacccat ttcccttttt caagatcttt gtagtgttcg
     3661 aaaaagtgag cgatctgctc aatcagcatg gatggaagat cacggtagct gcgaatgttt
     3721 gaatagaacg gatggagatc gtcgactgga accgctagaa ccttttcgtc cagacctgct
     3781 tcatcttgca ttagcagcgc gccgacggga cggcaccgga tcaccgcgcc tggaacaacc
     3841 gggacattgc caacaaccag gatgtcacac ggatcaccgt cctctgaaag agtgtgcggg
     3901 atgaatccgt aattgcccgg atagaacatt gccgtgtgaa gaaagcgatc cacgaacatc
     3961 gcgccggacg ctttatcgag ttcgtattta actggaacgc cgccaaccgg aatttctata
     4021 accgcatgta ggtcaatagg cgggtttcgg cctgtagtta ttttagagat gtccatagtc
     4081 attcaccttt ttaatagtcc tgatgaagcg aggccgctga agattccaat catcgaaaga
     4141 gcgaccgatg cgccggcctg gcagcgcagc cggcgagtaa ggcgagtgca aactggttgg
     4201 taggcgaaat agccgagact gctatccagt tcagctcggt ggatcaagtt cgagaccgtt
     4261 cggagctttc ggacggaact gcgcataaat cttcttcggc gcggcatcaa aatacttcag
     4321 gagagcgggg cagagccagc cctccatttc cagttcaggg cagtagtacc agttcccgct
     4381 gaactcttca cgacgccaat cgaactcagc ttgatatccg ggaaacggtt gtgccgagaa
     4441 gagcagggtg aaacccgaca cagcattggg gatctgttga accaaccgct cgatgatcgc
     4501 atcggcgccg gatacgaagg gttcttgcct aagccccacc gcagggtcgt cgaagacc
//
LOCUS       DASUVS010000135         7859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_922542, whole genome shotgun sequence.
ACCESSION   DASUVS010000135 DASUVS010000000
VERSION     DASUVS010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922542"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VF182_07760"
     CDS             <1..743
                     /locus_tag="VF182_07760"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX6437011.1"
                     /translation="GRLHAIMITLHADVVGSLLRSAELLAAQKQLAAGTLSTARFKEI
                     EDHAVDEAVALQENAGLEIVTDGEMRRQSFQSQMTAAVDGFGAFGLDAFLWGDWYDAE
                     GVHRQRRPDKLGVVEKLRRRRYLSADEFSYLKKKTSRIPKVTIPSPTLWANYWSPKYS
                     RTAYPTLDAFLADIVSILREEVTELVRLGGTYIQIDAPHYGLLLDARTRVFYENLGWS
                     LNRWLSLGLELDNAVVDGFRDVTFGFHV"
     gene            768..1124
                     /locus_tag="VF182_07765"
     CDS             768..1124
                     /locus_tag="VF182_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin-B12 independent methionine synthase"
                     /protein_id="HEX6437012.1"
                     /translation="MSQGGYEAISGELFRKLKPERLLLEFDDERSGSFEALREVPEDK
                     IIVLGLITTKQPELESLESLIGKVTEAARYVPLERLAVSPQCGFASSVVGNNISFEDQ
                     RRKLQLVIKTADALWS"
     gene            complement(1199..2275)
                     /locus_tag="VF182_07770"
     CDS             complement(1199..2275)
                     /locus_tag="VF182_07770"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6437013.1"
                     /translation="MKPFLVVDADGHVEESSKGLQKYLKPENRGRPLWTSDSWDRNFG
                     GALGKVNEDPHVQLEDMDADGIDVQVIYPTRGISLSAAREVDLAVDLAHAYNDWLADF
                     CSVNPARLKGVALVALQDVGAAIAEARRAVEQLGFIGVMMPTNVLDQDIGHKQFWPFY
                     EAVEGLEVGLGFHGGIRASQRMHGRFGSFIAVHTLAFPLECMVALTGMIYAGVPELFP
                     KLRVAALEASCGWVPFLMDRMDEEFEKRGSREAPLLKAKPSEYMTNGQFYYAFELEES
                     TLPYVAERVGSDKLLYSSDYPHWDTSWPNTVRTVRERQDITEAQKKQFLSENAQRFYG
                     FSTEAPMRAGLHQTIDKETASPRG"
     gene            complement(2323..3327)
                     /locus_tag="VF182_07775"
     CDS             complement(2323..3327)
                     /locus_tag="VF182_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437014.1"
                     /translation="MRSWHIQSMIIVVSFLSSLLMPSFISAADKLSALYSAQSVSYSL
                     PWIAQDAGLFRKHNLDFQLVYIPSSGVATAALLGGDVEIALAGGVGIVRAFAQGASDL
                     VFIGGFKNALTHSIVAKPELTKPEDLRGKKIGITRLGSNSHYFAVQALRRMGLDASRD
                     VTLIQAGGEPEIVAALINGAIDAGSITTPADNRAVAQGFRYLLFGPDLQIAYAAANIV
                     TRRSMIAKRPQAATAFMQVMAEAAKILHTNKEFTYKVLGKYLGISDPKIIDAAFATEI
                     KVMERRLDVKPEGIQAILEEVAKTDPRAKTIRPQDLIDRRFLDEMTKSGSFDKLWASK
                     "
     gene            3788..5224
                     /locus_tag="VF182_07780"
     CDS             3788..5224
                     /locus_tag="VF182_07780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6437015.1"
                     /translation="MNLQNHLPDSRSLIDERARRLTLINSVIGVTVAGISTRVFMISV
                     PTLATALGTDILGISWALIVYQMAGIGLGVICGRLGDIYGHQKMFGSGMGIMAVGSLL
                     CGLSQDVFQLILFRFVQGVGGAMIQSSGRTLAFRAMPAGSEGKAQGLMAMSHQFGFFI
                     GPPIGGLIIDLVHWRGIFFLLFVPSLVGMALCFMTGRSIAASDSRRERIDYRGASIFL
                     GLTVLVTMLLDQKIAEVLGEYSRALLAVTFVGVLWSFIAHEGRTSSPMIDLAFFSVPA
                     FGYGSVGLLICCITQGLVTFVTPFYLQEVLKLTPTFMGMIFLAPSLLSMVLSPVSGAL
                     TDRIGARLLLMTGVLILMTAFLIGANLGADSHWLWPAVLLALTGVGSAFFNTPSQAVM
                     ISALPKEHWGTAIGIINGTFGLGQMLGISLSGIFLTVAFRYYSGIPGTTPDPGNTHIF
                     VASMNVTYLFALAIIVIPLLTSMKMKKK"
     gene            5598..6461
                     /locus_tag="VF182_07785"
     CDS             5598..6461
                     /locus_tag="VF182_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437016.1"
                     /translation="MKKSIILALVGAFNFLTLFAVAPVSLAADSPSRKSKELDTPAVF
                     QAAGPSAESIQGAVDEFRAALGEPNNANTPGPLANGRREINWDGGGNNDTTTPPVTPF
                     NVFLDSRGGQFTTPGVGLSQAPPSGGATNGLAGLFNNPTYADTFTAFSPLRLFTPVGS
                     NITEALFFVPGFMGQFPATVRGFGAVFTDVDEPNGLNARLRRGNPRGASTLIEYFDQH
                     GNSIFSGFVPASPGDGTLSFFGIVFDDARIARVRITTGNVAAGPNDDEENDIVMMDDF
                     FYGEPQPLSLN"
     gene            complement(6649..7716)
                     /locus_tag="VF182_07790"
     CDS             complement(6649..7716)
                     /locus_tag="VF182_07790"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX6437017.1"
                     /translation="MNHVESIELLVIPQVSDQVLERIGKVDQRIKLVDVRGWFDVEIR
                     ASWPQWTIDRYLGNRAHPASTLEQRNRALASAQVVLLGWPPLKDLFSRAPRLKWVHEL
                     PAGASNLVGTDLWESDIIVTTSRGVTNKRPMAEYVLGSFLHFSRGLHWSYRDQRQHRF
                     EHQIYNPIMLRDKTVCVVGAGGIGREVAKLCAAAGMRVVGTRRHADPESAAPPGFARL
                     ASADALHDLLRESEFVAICCPWTKATTHLIDGAAFAAMKSGTVLVNISRGEIIDENAL
                     IGALAEGKIRGAALDVYDGEFERPPDARLWDDERVIITPHVSAATDVSEHGGVDLFCE
                     NLIRFLESQPLENVIDWQRGY"
BASE COUNT         1657 a         2144 c         2203 g         1855 t
ORIGIN      
        1 ggggacgact gcacgcgatt atgatcacac tacatgccga tgtggtggga agtctgctgc
       61 gatctgcgga gctgcttgcc gcgcaaaaac aactcgccgc cggcactctc agcacggcgc
      121 gatttaagga aatcgaggat cacgccgtcg acgaggcggt tgcgttgcaa gaaaatgccg
      181 ggttggagat cgttacggat ggcgagatgc ggcggcaatc gtttcagagt caaatgacgg
      241 ccgcggtcga cggtttcggt gcgttcgggc tcgatgcttt tctctggggc gactggtacg
      301 acgcggaagg tgtgcatcgg caacggcggc cagataaatt gggtgtggtc gaaaagttaa
      361 ggcgccgtcg atatctctca gccgatgaat tttcctacct taagaagaag accagccgca
      421 ttcccaaagt gaccattccg agcccgacac tatgggccaa ttattggtcg ccaaaatatt
      481 cgcgaacggc gtatcccacg ctcgatgcgt ttctcgccga catcgtcagc attctgcgcg
      541 aggaagttac cgaactcgtg cgcctcggcg gcacttacat ccaaatcgac gcgccgcatt
      601 atggcctgtt actcgatgcc cgaacgcgcg ttttctacga aaaccttggc tggagtctca
      661 atcggtggct ctctttgggc ctcgaactgg acaacgcggt ggtggacggg tttcgcgatg
      721 tgactttcgg gtttcacgtc tgacgcggta accaaggcag ccgttggttg tcgcagggag
      781 gatatgaagc tatcagcggc gaattgtttc gcaagttaaa gccagagcga ttgcttttgg
      841 aattcgacga tgagcgctcc ggctcgtttg aggctctcag agaagtgcct gaagacaaga
      901 tcatcgtcct cggcttgatc acaacgaaac agcctgagct cgagtctctg gagtcgctca
      961 tagggaaagt caccgaagcg gctcgttacg tgccgctgga gcgtttggca gtcagcccgc
     1021 agtgcggctt tgcttcttcg gtcgttggca acaatatctc gtttgaagac caacggcgta
     1081 aactgcagct cgtgattaaa acagcagacg ctctatggag ctaggccgca ttatcacatc
     1141 atggtcgagg agtaagccct tcgaggtaaa cgcaatgagc ctgtgcctgg gatggaagct
     1201 atccgcgcgg gctagctgtt tctttatcaa ttgtttggtg taggcctgcc cgcatcggcg
     1261 cttctgtgct gaaaccgtaa aacctctgag cattctccga cagaaattgt ttcttctggg
     1321 cttcggtaat gtcttgacgc tcccggaccg ttcgtaccgt gttgggccat gacgtatccc
     1381 agtgcggata gtccgaagag tagagtaatt tatcggatcc cactcgctca gcaacatagg
     1441 gcaacgtcga ttcttcaagt tcgaacgcat agtagaactg gccattggtc atgtactcgc
     1501 tcggttttgc cttgagtagg ggcgcttctc tgctgccgcg tttttcaaac tcctcatcca
     1561 tccggtccat taagaatggt acccacccac agctcgcttc cagagcggcg acgcgcagct
     1621 tcgggaacaa ttccggaaca ccggcataaa tcattccggt cagagcgacc atgcattcaa
     1681 ggggaaacgc caacgtatgg accgctatga agctgccgaa tcgaccgtgc atccgttgcg
     1741 aagcgcgaat gccgccgtga aacccgaggc caacctcgag tccctcgacc gcttcgtaga
     1801 acggccagaa ttgtttgtgg ccgatgtcct gatcgagcac attggtcggc atcatcacgc
     1861 cgatgaatcc cagttgctcg acggcgcggc gcgcctccgc gatagcggca cctacgtcct
     1921 ggagtgcgac cagcgcgact cctttgagcc gcgccgggtt gaccgagcag aaatcggcaa
     1981 gccaatcatt gtatgcgtgg gcgaggtcca ccgccaaatc cacttctctt gccgcgctga
     2041 gagaaatccc gcgtgtcggg tagatcacct ggacatcgat gccgtcggcg tccatgtctt
     2101 cgagttggac atgaggatct tcattgactt tgcccaaggc gccgccgaag ttgcgatccc
     2161 aggagtcgct ggtccagagc gggcgaccgc gattttccgg ctttaaatat ttttgcagtc
     2221 ctttcgatga ttcttcgacg tgtccgtcgg cgtcaacgac cagaaaaggt ttcatgaaaa
     2281 cactcctcca ttcaatctaa gctgagtttt gctcggcaac tctcatttgc ttgcccatag
     2341 tttgtcaaaa gagccgctct tggtcatttc atcgaggaag cgccggtcga tcaggtcttg
     2401 cggccggata gttttcgctc gcgggtctgt cttagcaact tcttcgagaa tcgcttgaat
     2461 accttccggt ttaacatcca agcgtcgctc catcactttg atctcggttg cgaacgcggc
     2521 atcgataatc ttcgggtcgc tgatgcccag gtatttgccc aggaccttgt aggtaaattc
     2581 tttgttggtg tggaggattt ttgccgcttc ggccatgacc tgcataaacg cggtcgcagc
     2641 ctgtggccgt ttagcgatca tcgaacggcg agtgacgatg ttggcggcgg cataggcaat
     2701 ttgtaagtcg gggccgaaca ataaataacg aaaaccctgg gcgaccgctc gattgtccgc
     2761 cggcgtggta atcgatccgg catcgatggc gccgttgatg agagccgcga cgatttccgg
     2821 ctcgccgccg gcttgaataa gcgtcacatc gcgtgatgcg tccaagccca tgcgtcgcaa
     2881 tgcctggacg gcgaaatagt gactgttgct gcccagccgt gtgatgccga tcttctttcc
     2941 tctgagatcc tcaggcttgg tcagttccgg tttagcgacg atgctatggg tcagggcatt
     3001 tttgaagccg ccgataaaca ccagatcgct ggccccttga gcaaaggcgc gcacaatgcc
     3061 gactcctcct gccagggcaa tctcaacgtc gccgccgagc agagccgccg tggctacacc
     3121 cgacgaaggg atatagacga gctgaaaatc cagattgtgt ttgcgaaaaa gaccggcatc
     3181 ctgcgcgatc catgggagcg agtaggacac cgactgagcc gaatagagcg cgctgagctt
     3241 gtcagccgcc gaaataaacg atggcatgag cagagatgat aggaatgata cgacgatgat
     3301 catagattga atgtgccaac tgcgcatcct attcaccttt ctctataacc cattgtctta
     3361 ctttggggca taacatccgg tgctcagcaa ggatgctcga tcagcgaagt atcgccagta
     3421 ttccggatcc ttatcgcgaa gacttggcac actgtcaaga aaatttccag catggtattg
     3481 atcgactgcg ttgcccggta tgtcgtaact agagcagcaa gcggcgtgct gagtcgggcg
     3541 cctatgaagt tgcgccttca aggctcgccc ccgtaagact cgaaaacggg cgcgacagaa
     3601 aagtctgtca tcgtgcaatt aaaccgagcc tctcctataa aatgcgacta ggaccgagta
     3661 cgaatccggc gatcttcgca cgtcacatcg ttgtgcgcgt ctggcggaaa cactcgactc
     3721 ggacggtcca cctctatgcg catcttagaa aaccgtcaac atcttaatgc ctgtaagagt
     3781 tgcccctttg aacttgcaaa atcacttacc cgatagtcga tccctcatag atgaacgggc
     3841 gcgccggttg actctgatca attcggtcat cggcgttacc gtcgccggaa tttcgacccg
     3901 tgtgttcatg atctcagtgc cgacgttggc gacggcgctg ggaaccgaca tcctgggcat
     3961 ttcatgggca ttgatcgttt atcagatggc gggcatcgga ctcggcgtca tctgcggccg
     4021 gctgggcgat atctacggcc atcaaaaaat gttcggctcc gggatgggca tcatggctgt
     4081 tggatcgctt ctctgtggcc tgtcgcagga tgttttccag ttgatcctgt ttcgctttgt
     4141 ccaaggagtt ggcggcgcga tgattcaatc ttccggccgt acactggcgt ttcgcgccat
     4201 gcctgcaggt tccgagggca aggctcaggg attgatggcg atgtcacatc agtttggatt
     4261 tttcatcggc ccgccgatcg gcggcctgat catcgatctg gttcattggc gcggcatttt
     4321 ttttctgctg ttcgttccga gcctggtcgg catggcgctc tgttttatga ccggacgctc
     4381 gattgcggca tcagattcgc gccgcgagcg gattgattac cggggagcat cgatcttcct
     4441 cggattaacc gtgctggtga cgatgcttct cgatcaaaaa attgccgagg tgctgggcga
     4501 atatagccgg gctcttttgg ctgtgacttt tgtgggagtg ctatggagtt tcattgcgca
     4561 tgagggaaga acctcgagcc cgatgatcga tctggctttt ttctcggttc ctgcgttcgg
     4621 ctacggctcg gtcggcttac tcatttgctg catcacacaa gggctggtta cgtttgtcac
     4681 gcctttctac cttcaggagg tgctaaagct tacgcccacc tttatgggaa tgatcttttt
     4741 ggcgccatcg cttctcagca tggtgttatc gccggtaagc ggtgccttaa ccgatcgcat
     4801 cggcgcccgt ttgctcttga tgaccggtgt gctcatcctg atgacggctt ttctcatcgg
     4861 agcgaatctt ggcgccgact ctcactggct ctggcccgct gtcctgctcg cgcttacagg
     4921 ggtagggtcg gcttttttca acacgccttc gcaggcggtg atgattagcg cgttgcctaa
     4981 agagcattgg ggcacagcga tcgggatcat caatggaacc tttgggctgg ggcagatgct
     5041 cggcatatcg ctgagcggaa tttttttgac cgtggcgttc cgatactatt cagggatccc
     5101 cgggacaacg cccgatcccg gaaatacgca catctttgtc gcttcgatga atgtgactta
     5161 tttgtttgcg ctggcaatta tcgtgattcc gctgctgacc tcgatgaaga tgaaaaaaaa
     5221 gtagacaacg actcgtactc gctgcgcgag atttttcttg acctattcca agcctgtgat
     5281 aaacgggcaa gagaaactcc acattgctcg tggctgctgt gcgtgctgtg tctgtggtgg
     5341 tcgttttacg actgtcgccg atgagaggca tgccgatgag gccattgctg cttgtccgtt
     5401 cgcaccagtt tcgatttgcg gctaacactc tcttccgaat tgattctccc ctcagagtgc
     5461 aactcatcag ccgtgcgaga gctcccccgg gcgcgcaaca atctacgacg accaggcgga
     5521 gtgcggtaca aataaacccg atccattgga ctaaaatgtt gttacaatca agagaataaa
     5581 gaaaggagca tgcgaccatg aagaagagta tcattctggc gctcgttggc gcctttaact
     5641 ttttgactct cttcgcggtg gctccggtga gcctagcagc agattcgcca agcaggaaat
     5701 caaaggagct tgatacgccg gctgtattcc aggcggccgg gccaagcgca gagtcgattc
     5761 agggtgcggt cgatgagttc cgtgctgctc tcggagaacc gaacaacgcc aacactcccg
     5821 ggccgctcgc aaacggtcgg cgtgagatca actgggacgg tggcggtaac aatgacacca
     5881 ctacgcctcc ggtaacgccg ttcaacgtgt ttctggattc gcgcggcggt cagttcacca
     5941 cacctggggt gggactttca caggcgcctc cttcaggcgg cgcgacgaat ggcctcgcgg
     6001 ggctcttcaa caatccgacc tacgccgaca cctttaccgc attcagcccg ttgcgcctgt
     6061 tcacccccgt cggcagcaac atcactgagg cattattctt tgtgcccggc tttatgggac
     6121 aatttcccgc aaccgttcgt ggcttcggcg ccgtctttac agatgttgac gagccaaatg
     6181 gtcttaacgc tcggctgaga cgcggaaacc cgcgcggtgc aagcacgttg attgagtact
     6241 tcgatcagca cggcaactcg atattcagcg gttttgttcc ggcttcgccc ggagatggca
     6301 ctctttcttt cttcggcatc gtctttgacg acgctcgcat cgcgcgggtc cggatcacga
     6361 cgggcaacgt agccgcgggg ccgaatgatg atgaagaaaa cgacatagtt atgatggatg
     6421 acttttttta cggcgagcca cagccgcttt cgttgaatta gcgggataat gtacgagctt
     6481 ccatcctagt aaattggagt cggggtcatg tcagagcgcg aaatcataag ccgaaaaacg
     6541 acatggccct gactcccgaa gtgatagtgt ttcaactttt tggcgtgcca atactcagag
     6601 agtagtgggc ccggtaaaac tccgactgtc gtaggccacg tttgtacctt agtatccccg
     6661 ctgccagtca atgacattct ccaacggctg gctttcgaga aagcggatca ggttttcgca
     6721 aaacaagtct acgccgccgt gttccgacac gtcggttgcc gcggaaacgt ggggcgtgat
     6781 gatgacgcgc tcatcgtccc acaagcgcgc atcgggcggg cgctcgaact caccgtcgta
     6841 aacatcgaga gcggcgccgc gaatcttgcc ttcggccaat gcaccgatca aggcattttc
     6901 atcgattatt tcgccgcggg agatgttgac taaaacggtc ccggatttca tcgccgcaaa
     6961 cgccgctccg tcgatcaagt gtgtggtcgc ttttgtccag ggacaacaga tggcgacaaa
     7021 ttcactctcg cgcagcaaat cgtgcagagc gtcggcggat gccaaacggg caaatcctgg
     7081 cggggccgcg gactctggat cggcatggcg gcgggtaccg acgacgcgca tcccggcggc
     7141 tgcgcagagc tttgccactt cccggccgat gccgccggcg ccgacaacgc agaccgtctt
     7201 atcgcggagc atgatcggat tataaatttg atgctcgaag cgatgttgcc gttgatcgcg
     7261 gtagctccaa tgaagacctc gagagaagtg gagaaaactg cccaggacat actcggccat
     7321 cggacgcttg ttggtcactc cgcgtgaggt ggtcacgata atatcgcttt cccacaaatc
     7381 ggtgccaacg agattactcg ctccggccgg gagttcatgg acccacttca ggcgaggtgc
     7441 gcggctaaat aaatctttca acggcggcca gcccagaagg acgacctgcg ccgaggccaa
     7501 ggcgcggtta cgctgctcga gcgtgctcgc cggatgagct cgattgccca gataacgatc
     7561 gatggtccac tgcggccagc tggcgcggat ctcgacgtca aaccatcctc ggacatcgac
     7621 aagtttgatc cgctggtcga ccttgccgat gcgctcgagt acctgatcgg agacctgcgg
     7681 gatcacgagt aattcaatgc tctccacgtg gttcattgac ctagcacacc agattatttc
     7741 ttatagttat ccaagaacca acattacata ggaggcacat gcggacaacg aagccttgct
     7801 tgcaaatatg caagctcgta tttcttgctt gtctacttcg gggtgaagag ttttcgcag
//
LOCUS       DASUVS010000136        11908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_49311, whole genome shotgun sequence.
ACCESSION   DASUVS010000136 DASUVS010000000
VERSION     DASUVS010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11908
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49311"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1056)
                     /locus_tag="VF182_07795"
     CDS             complement(64..1056)
                     /locus_tag="VF182_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437018.1"
                     /translation="MKLLYSLVVSLCLLANDSSLAHAQKLEKLVIGYPAPVASLGLID
                     VTRKAGLFKKHGLDVDLVYIQGSPILTAAMMSGQVPLSFLGGAAIVASAVGGADTVYL
                     GCAINTLYWRVFSSPEIKDVADLKGKRIGVTTIGSQEDSVVRYLLRERGLNPERDVTV
                     VAVGSAPTRLAALSKGFVQASTFIPPQDIAAEKLGLNVLIDMSKLGLYNPGSCFASTK
                     TYVKSHRDTVMRVMKAFVEGLKFYRENKEFALKVTADFAQNRNIDVLNSTYDVVTRYQ
                     DRVPYVNMQGIEFMLKTLEVRDPRAKTFDAANVVDSSFIQELEKTGFIDSVWKK"
     gene            complement(1059..2045)
                     /locus_tag="VF182_07800"
     CDS             complement(1059..2045)
                     /locus_tag="VF182_07800"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437019.1"
                     /translation="MTLRFFSIVPAIGAVLIASLMNGRALALDDVKVHTSGSTSANVV
                     IYKIAQEQGFYKEENLNVLTIAATSQAGIQGLVGGSFDFSQILGQTSAAILHGAPLKN
                     VMVFDTKPLFWFFGSKKIKRVQDLKGGKLVGVSSLGANTDQMTREILAMNGIDPRKDV
                     VIQGTGTGAVRLAALMGGSLDAAITNPTESLVARKQGLNELFFYGDYDLNIVSGGAAL
                     TERMLKEKPDLVRRFLRGTLRALLWFRTNEKNAVARMAEGFKISPDDALGIYKATLKS
                     YTLDGTISRELQEKIVTFQRKQLKVEKEVPPETVYDFTILRALNEERKKSRS"
     gene            complement(2051..3619)
                     /locus_tag="VF182_07805"
     CDS             complement(2051..3619)
                     /locus_tag="VF182_07805"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6437020.1"
                     /translation="MGYYPSLREYVQTLESREKLFRIEREVCRETELHPLVRWQFRGL
                     PENERRAFLFAKVSSVAGRKYKMSVLSGACASSRAIYALGMGCREEEIFDRWINAQKN
                     PIEPIVVPEGPVQEIDIDTNLDDAVDGIPVPLGNPGFDSSLRLTAALWVTADPETGER
                     NVGIYSGYIRQGGKISVGLGAGKDSRIHLDKAREKKLPLKAAVVIGPTPNLIYAAAST
                     FPYGVDEYAVAGAIAGAPLELVRCRTADLYVPAHAEMVLEGEISPDQMDHNWPFGEFS
                     GYMAGRGRYPRFTVKHITQRRDPILLDLVAAHPPCEGSKLKQIATEAIFYHGLKDNVG
                     MSGIRQVAIHEESGGWGLFVIQLKARQPAHAWQALNSAASLTPGIGKIFIVVDEDIDP
                     HDLDAVMWALVFRVQPHRDIQTVTGRRPMMDPSAVSPAEPGWHHHYPEPHGSSALLIN
                     ALKKWNYPPVALPAKGYMESARRLWEELGLPQLKPKSPWHGYELGHWPEELRNAAAAV
                     AAGRSPDESTKKEP"
     gene            complement(3630..4679)
                     /locus_tag="VF182_07810"
     CDS             complement(3630..4679)
                     /locus_tag="VF182_07810"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437021.1"
                     /translation="MPCRFREELFFAAFDRTRWTLARRHLTLLVIVAASVLGFSAKQA
                     RTAEKIRVAYPAVAPGLTPSWVTAEAGIWRKYGLDVETILVSGGARALPALISNSVQF
                     LMGSDTGVTTARLQGLPVVRLGVTMNTLGSALVTQPNIQSVRDLKGKTLGISRGRDAS
                     YIRLAKLLRDHGLNPNEDVKLLPIGGGEGGRLSALKAGVIQGTMLFPPLDLIASNDGL
                     KVLEKFDVATPGGGINTTAALLKQNRALAINFLKGYMEGIHYMAYHKDQSLKILQRYF
                     NNSDITAMGYLYDETTRRLEKDLHASQESIRFHLDLAALDDPRAAKLGEKDFWDASVV
                     EEIRRSGFIDQLYNK"
     gene            complement(4763..5611)
                     /locus_tag="VF182_07815"
     CDS             complement(4763..5611)
                     /locus_tag="VF182_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6437022.1"
                     /translation="MKSRIDQISRSVYAVIGPEGSTNFGIVKGSDNSAAVIDADIRRI
                     DEIEDALQLTGCSKVSYLIDTHEHFDHTSANFYFAQRGIPVVASAGCAAAMREEGGAD
                     FARMMKPVPELYERFPGLCLTLPDVVFGHSTQIAFPGVTLHLRYCAENGHSHSKGDTT
                     IYFEEEEILVAGDLLYTEVHPVTFFGNIPNWLTALKPLFESHYRQLLPGHGPAVAGEQ
                     AGRAYFKKMYDYLEDFHGRMVEVKAGRQNAEDVAQHMLSGAYASFGKTRMVERNINHF
                     LTGRWF"
     gene            5726..6502
                     /locus_tag="VF182_07820"
     CDS             5726..6502
                     /locus_tag="VF182_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6437023.1"
                     /translation="MIKEGKFTLVEKRGPVAKITINRPDKRNALSRDVIREMLGAFEE
                     IRNDESIAVVLTSGAGDIAYCAGRDLSEFPTEGGAAVEPERRAQPRAYHVAEAIRTFP
                     KVTIAVVNGFCLGAGITILLPHDLAIASDRAQFGLPEIKRGFLPYPIIATMFKTLIPT
                     KLAFELILTGENWDATKSMEAGLINRVVPHKELDQAAWQWGEEIGRFDKTTLKYCKMA
                     AHASMEATSVPLAAEIAWLMQEEHSRVNPKAYAGTKQFHK"
     gene            6644..7570
                     /locus_tag="VF182_07825"
     CDS             6644..7570
                     /locus_tag="VF182_07825"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX6437024.1"
                     /translation="MSIKLEGILSANVTPFKENYDLDEDGLRRLIRYQARPEGISAMV
                     CNAGAGEGAALTRQERVRIIEIIGEEIRSDQCIVAGIEALSTREAIDQAKEAKAAGAA
                     AIMLTPPHVYDWSSTANPEFAIQYFHDVANAVEIPITIFHYPASTASGYLPDTAIRIA
                     REVDSVVAIKVASGANIRRYEQVLRGMRALPRQISVLATSSLFQHFITGADGALTGFA
                     NFAPQFCVDLYNAVKAGDLDKARRLHEINWALEAAVYKAPNVYKHSRYKVAAYFAGLL
                     DNMIVRAPQVPVPEGEVKLIHDAMEKLAMLKR"
     gene            7601..8566
                     /locus_tag="VF182_07830"
     CDS             7601..8566
                     /locus_tag="VF182_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437025.1"
                     /translation="MNKFISIVLVVCAVIPASGFAQERRKIRISNANLSFTALPLVAA
                     REWKLFDQQGLDIEVILMRSSAAAAALASGDLDYQSGIGTASVSATLSGLDARALWAS
                     TNRIVYWLMANPQYKSVRDLRQKKIGVTGLGGTSHVALTAALEKSGLGAKDYVAVSVP
                     SAQLVQALESKFVDAASLNPPFMFFAQRRGLAKLLDIGALVEMPSGGLTALSKTMKAR
                     PDEVKRVVRALQTAKSMMLKSRERTLDLITGVLRMDRETAAETYKLVESNFNDTGIPS
                     PEGMANIVKAIMAEGRFTDRNVSFEEVAEPRFAIEVAKELGYKVP"
     gene            complement(8579..9526)
                     /locus_tag="VF182_07835"
     CDS             complement(8579..9526)
                     /locus_tag="VF182_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437026.1"
                     /translation="MARDLELTLLLADYHRTRPILDGDVTARGIKLQPRRAETGEACM
                     RPVYEEFDITEMSLSWYMMARCRKEPVIALPIFPLRMQIHPYIFCSPSSAIERPEDLK
                     GKKIGMDQYRLTVGLWARGILQEDYGVSPEECEWFTSEREGAGYQPSPNVKITVTHQP
                     AEAMLLKGEIDALIPPNIVPSFRAKDARIRRLFKDPRAIVNEYFRRTKIFPITHTLVV
                     RQTLFDENPWLVSSLLDAFNEAEERCRKSYEYAKRLAFPSAVLILEEEEEVFGDNPWS
                     HGLTAQNQVVLDKFVQYAHEQGYIPERPPLGELFAPLGS"
     gene            complement(9545..10525)
                     /locus_tag="VF182_07840"
     CDS             complement(9545..10525)
                     /locus_tag="VF182_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437027.1"
                     /translation="MPLLSAKKMAVSAYCLMLSALFLPARVSAKNVNFAYNTPTLSGT
                     LPVVVAQDFGFFAAEGLEVKTVFIRGGPTAMAALVGGGVDYTFVAGVAAVRAIAQNAP
                     MLIVSGMQPYMDYTLIGGKGLSTVNDLRGKVVGVTGPGGVAEFAAVEGLAKKGMVRDR
                     DYKILYGVGNSPARAQALEIGKIHASPFSFLERLELEKKGFPAIFDIGEAIPGFPFVV
                     IASGKQKIDNDPEGIVALLRAINRGLEFLKKNREKVAEAVINKNTFGDPTMVRRVINQ
                     FADLYSTSISRKDIDALINATRIEAEAKKFGGPEKFFTRQFLTKALGQSR"
     gene            complement(10529..11509)
                     /locus_tag="VF182_07845"
     CDS             complement(10529..11509)
                     /locus_tag="VF182_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437028.1"
                     /translation="MKQFLGLTTLLLLAVFLFGPAVGAADRVKASYAAVNASQSPLWV
                     AQDRGFFAKYGIEADLVFISSGSLNVQALVGNTVQFAAGGPAALEARLRGPKLVTIAN
                     PLTVLASNLVSHPDIKSIADLKGKVGAVSRFASSSHQGLRYLFRKNGLSESDLKLLQL
                     GGDSGRLTGLTTGKVQYTFLGAAASEQARKQGLTILATAKQMAIPFPWTSVVVHETWL
                     ESNRELAYRYVKAVTEAVSFMKRNRPESERIISKYMKLTDPNLVAIEYNFNVALFPDL
                     PYPTLDGVRLILDNLATENPEHGRRDPKEFVDSSIVDRLKQEKFLESLGK"
BASE COUNT         2579 a         3264 c         3400 g         2665 t
ORIGIN      
        1 cgcgggtttg gttgcgaaat gttgcgcgtt acgcggcaga gcttcaccga tgatcatgcc
       61 gaattatttc ttccacaccg aatcgataaa gccggttttt tccaattcct gaataaagct
      121 cgaatcgact acattggctg catcaaatgt tttagcccgc ggatcgcgca cctccagagt
      181 tttgagcatg aactcgatac cttgcatatt gacgtaagga acgcggtctt ggtatcgggt
      241 gacgacgtcg taagtggagt taagcacgtc gatgtttcta ttctgcgcga agtcggcggt
      301 taccttgaga gcgaattctt tattctcccg ataaaactta agtccctcga caaatgcctt
      361 catgacacgc atcacggtgt cgcgatggct ttttacgtag gtctttgtgc tggcaaagca
      421 actgccggga ttgtacaaac ccaatttgct catatcgatc aaaacattca ggcccagctt
      481 ttcggcggcg atgtcttgcg gcgggatgaa agtcgaggcc tgcacgaagc ctttgctcag
      541 cgccgccaac cgggtgggcg ccgagcccac ggcaacgacc gtcacgtcgc gctccggatt
      601 gagaccccgt tctctgagca gatagcgcac cacgctgtct tcttgtgaac cgattgtggt
      661 gacaccgatt ctcttcccct tgagatcggc gacatccttg atttcaggag atgaaaagac
      721 gcgccagtag agcgtattga tcgcgcaccc caaatagacg gtgtcggcgc cgccgaccgc
      781 cgaagctacg atggcggcgc cgccaagaaa gctcagcggc acctgtcccg acatcatcgc
      841 tgccgtaagg atcgggcttc cttgaatata gacgaggtcg acgtcgagcc cgtgtttttt
      901 gaacagaccg gctttacgcg tgacgtcgat aagacccaac gaggccaccg gagcaggata
      961 gccgatgacg agcttttcca atttctgcgc atgagcgagg ctggagtcgt ttgccaacag
     1021 gcacagcgaa acaaccagtg aataaagaag tttcattttc atgatctcga ttttttacgc
     1081 tcttcgttga gcgcccgcaa gatcgtgaag tcatagacag tttcgggcgg gacttccttt
     1141 tcaactttaa gctgcttgcg ctgaaaagtt acaatttttt cctggagctc gcgtgaaatg
     1201 gtgccgtcga gtgtatagct cttgagcgtt gctttataga tgccgagcgc gtcatcaggc
     1261 gaaatcttga acccttcggc catgcgcgcc acggcgtttt tttcattggt ccgaaaccac
     1321 aacagcgctc tgagcgtgcc gcgcagaaaa cgccgaacca gatcgggctt ttccttgagc
     1381 attcgttccg tgagcgcggc tccgcctgag acgatgttga gatcgtaatc gccatagaaa
     1441 aaaagctcgt tcagcccctg cttacgtgct acgaggctct cggtgggatt tgtaatcgcg
     1501 gcgtcgagac tgccgcccat caaggccgcc aaccgcacgg cgccggttcc ggttccttga
     1561 atcaccacgt ctttgcgcgg atcgataccg ttcatcgcca ggatctcacg ggtcatttga
     1621 tcggtgttgg cgccgaggct cgacacgccg accaatttgc caccctttaa atcctgcacg
     1681 cgcttgattt tcttgctgcc gaaaaaccag aaaagcggct tggtatcgaa caccataaca
     1741 ttcttgagcg gagcgccatg tagaatcgcc gcggaggttt ggccgagaat ctggctaaag
     1801 tcaaaactgc cgcccaccaa accctggatg ccggcctggg aggttgcagc gatggtcaga
     1861 acgttgaggt tttcttcttt atagaagcct tgctcctgag cgattttata gatgaccaca
     1921 ttggcgctgg tgctgccgga tgtatgaacc ttcacatcat cgagcgccag cgctcggccg
     1981 ttcatcaagg acgctatcag aacagcaccg atcgcgggca cgatcgaaaa aaatctgagt
     2041 gtcatccgac ttatggttcc tttttagtac tctcgtccgg gctccggccg gccgccaccg
     2101 cggccgcagc gttgcgcaat tcctcgggcc agtgcccgag ctcgtatccg tgccatggcg
     2161 atttcggctt gagttgcggc aatcccagtt cctcccacaa ccggcgcgcg ctttccatgt
     2221 accctttggc gggcagagcg acgggcggat agttccattt ttttagcgca ttgatgagca
     2281 gggccgaaga tccgtgtggc tcgggatagt gatggtgcca accgggctca gcgggtgaaa
     2341 ctgcagacgg atccatcatc ggccgcctgc cggttacggt ttggatgtcg cggtgcggct
     2401 gaactcgaaa aaccagcgcc cacatcactg catcgagatc gtgcggatcg atgtcttcgt
     2461 cgacgacgat gaatatcttg ccgatcccgg gcgtcaagct ggcggccgaa ttcagcgctt
     2521 gccaggcatg ggccggttgc cgggctttta gctggataac gaagagtccc cagccgccgc
     2581 tttcctcatg aatcgccacc tgcctgatgc cgctcatgcc gacgttgtct ttcaagccgt
     2641 ggtaaaaaat cgcttcggtc gcgatttgct tgagtttgct tccttcgcaa ggcggatgag
     2701 cagccacgag atcgagcaaa atcggatctc tgcgctgcgt gatatgcttg acggtaaagc
     2761 gcggatagcg gccgcgtccg gccatgtagc cggagaattc tccgaacggc cagttgtgat
     2821 ccatctggtc cggagaaatc tcgccctcca aaaccatttc agcgtgagcg ggcacataaa
     2881 gatcggcggt gcggcaacga accagctcca gaggcgcgcc ggcgatcgct cctgccaccg
     2941 catactcatc gacgccatag ggaaaagtag aagcggcggc atagatcaag ttcggcgtcg
     3001 gcccgattac tacggcggct ttgagcggca actttttctc gcgcgctttg tccaaatgaa
     3061 tgcgtgaatc tttgccggcg cccaagccga cggaaatttt cccgccctgg cgaatatagc
     3121 cgctgtagat gccgacgttg cgttcgccgg tttccggatc agcggtgacc cacagggccg
     3181 cggttaaacg cagcgaactg tcaaagcccg gattacccaa tggaactgga atgccgtcga
     3241 cggcatcgtc gaggttcgtg tcgatgtcga tttcctgcac cggaccctcc ggcacgacaa
     3301 tcggctcgat cggatttttc tgggcgttga tccagcggtc gaagatctct tcctcccggc
     3361 atcccatccc caaagcataa attgcgcgtg aagaggcgca tgctcccgat agaacggaca
     3421 tcttgtattt gcggccggcg acgctcgaaa ctttggcgaa cagaaacgcc cgccgctcgt
     3481 tctccggaag gccgcggaat tgccagcgca ccaagggatg gagttcggtc tcgcggcaaa
     3541 cttcacgctc aatgcgaaaa agcttttcgc ggctttccaa tgtctgaacg tattctctga
     3601 gactcgggta ataacccatg cggtagctcc tatttgttat acagctggtc gataaatccg
     3661 gagcggcgaa tctcttcgac gacgctggcg tcccagaagt ccttttcgcc aagcttggcg
     3721 gcgcggggat cgtcgagggc ggccaaatca aggtggaagc gaatcgattc ttgactggcg
     3781 tggaggtcct tctcgagacg gcgggtggtt tcatcgtaga gatagcccat ggcagtgatg
     3841 tcagagttgt taaaatacct ctgcaaaatt ttcaggctct ggtctttgtg gtaagccata
     3901 tagtgaatcc cttccatgta gcctttgagg aaattgatcg ccagagcgcg gttctgtttg
     3961 agcaatgccg ccgtggtgtt gatcccgcca cccggtgttg cgacgtcgaa cttctcgaga
     4021 actttcaagc cgtcgttgct ggcgatgaga tccaacggcg ggaaaagcat cgttccctgg
     4081 atgactcccg ccttcaatgc ggataatcgg ccgccctcgc cgccgccaat cggtaagagc
     4141 ttaacgtctt cgttggggtt gaggccgtga tcgcgcagca gcttggcaag acgtatgtag
     4201 ctcgcatcgc gcccgcgcga gatgcccagg gtttttcctt tcaagtcgcg caccgactgg
     4261 atattgggtt gggtgaccag ggccgagccg agcgtgttca tcgtcacgcc cagtcgcacc
     4321 acgggtagac cttgcaagcg cgccgtggtg acgccggtat cggaacccat caaaaactga
     4381 acgctgttgc tgatcagtgc cggaagggcg cgcgcgccgc cgctgacgag aatggtttcc
     4441 acgtccaagc catatttccg ccagattccc gcctcggcgg tgacccatga aggcgtgagc
     4501 ccgggagcta ccgccggata ggcgacccgg attttttcgg cggtgcgagc ctgcttggcg
     4561 ctgaacccta aaaccgacgc ggcgacgatc acaagcaatg tgaggtgccg tcgagcaagc
     4621 gtccatcgtg tcctgtcaaa cgcagcgaaa aatagttctt ccctgaaacg gcacggcatc
     4681 gggaagatct ctcgccgtgc ttgcggggac gattctagat tcatatgaga gcgattgcca
     4741 ttctgaagaa cggtggcgcc atctaaaacc atcttcctgt gagaaaatga ttgatgtttc
     4801 tctccaccat ccgggtcttg ccgaacgatg cgtacgctcc gctgagcatg tgctgggcca
     4861 cgtcttcagc gttttgacgc ccggctttga cttcgaccat gcggccgtga aaatcctcca
     4921 agtagtcgta catctttttg aaataggctc tgcccgcctg ctcgcccgcc actgccggcc
     4981 catggccggg aaggagttgc cgatagtggc tttcgaagag cggcttcaag gccgttagcc
     5041 aattgggaat attaccaaag aacgttacag ggtgaacttc cgtgtagagc aggtcgccgg
     5101 caacgagaat ttcttcttct tcgaagtaaa tcgtcgtatc gcctttgctg tggctgtggc
     5161 cgttctcggc gcagtatctg agatgcagcg tgacccccgg gaacgcgatt tgagttgaat
     5221 ggccgaacac gacgtccggt aacgtcaaac acaagccggg aaaacgttca taaagttcgg
     5281 gcaccggctt catcatgcgc gcgaaatccg ctccgccttc ctcgcgcatg gccgcggcgc
     5341 agcccgcgct ggcgacgacg ggtatgccgc gctgcgcaaa ataaaaattc gccgaggtgt
     5401 ggtcgaagtg ctcgtgggtg tcgatgagat agctgacttt cgaacagccg gtgagctgca
     5461 gcgcgtcttc gatttcgtcg atgcggcgga tatctgcgtc gatgaccgcg gcgctgttgt
     5521 cgctgccttt gacgattccg aagttggtcg atccttccgg gccgatcacc gcatacacgg
     5581 atcgactgat ttgatctatt cgagatttca tagctccctc gatttatccg agtcgcggtg
     5641 gtgattcaag cgtcgacagg gattgaccgc aggcagcggc ggtgttaatc gcgtataaac
     5701 tcgcaaacta agaaagggct tgttcatgat caaggaagga aaatttacgc tcgtggaaaa
     5761 gcgcggcccg gtcgcgaaga tcacgatcaa cagaccggat aagagaaatg ccttgagccg
     5821 ggacgtgatt cgagagatgc tcggtgcttt tgaagaaatc cgtaacgacg agtctatcgc
     5881 agtggtgtta acctcgggcg cgggggacat tgcttactgc gccggccgcg atttatcgga
     5941 gttccccacc gagggcggcg ctgcagtgga gccggagcgc cgggctcagc cgcgcgccta
     6001 ccatgtagcg gaagccatta gaacttttcc gaaagtcact attgccgtcg taaatggctt
     6061 ttgcctgggc gccggtatca cgattctttt gcctcatgac ctcgctatcg cttcggatcg
     6121 agcgcagttc ggactgcctg agatcaagcg cggtttcctg ccttatccaa tcattgcgac
     6181 gatgttcaaa acccttattc cgaccaagtt ggctttcgag ttgattctca cgggagaaaa
     6241 ctgggacgcg acaaagtcga tggaagcggg attgatcaat cgcgtggtgc cgcacaaaga
     6301 actcgaccag gcggcatggc aatggggcga agaaatcgga cgattcgaca agaccacttt
     6361 gaaatattgt aaaatggcgg cgcatgcttc gatggaagcg accagcgtgc cattggcggc
     6421 agagattgcg tggttaatgc aggaagaaca tagcagagta aatcccaagg cttacgccgg
     6481 cacgaaacag tttcacaaat aatttgttcg caccgtttcc ctggccgaac aagtcggcag
     6541 gatcggctag gcgacgattc gtctcgactg gcgcgctgtt gaggctgcta aataccatga
     6601 ggaagcttcc gaccgcggct gcataatcag gaagagagtc gatatgagca tcaaacttga
     6661 aggcattctc agtgccaatg taacgccctt caaagagaac tatgacttgg acgaggacgg
     6721 cctgcgcaga ctcatccgtt accaggcccg gcccgaaggc atctcggcga tggtatgcaa
     6781 cgccggagca ggcgaggggg cggcgctcac ccgtcaggag cgggttagga tcatcgagat
     6841 catcggcgag gagattcgta gcgatcaatg catcgtcgct ggtatcgaag cgctatcgac
     6901 gcgcgaggct atcgatcagg cgaaggaagc caaagcggcg ggcgcggcgg ccatcatgct
     6961 tacgccgcca catgtttacg actggagctc cacagccaac ccggaatttg cgattcaata
     7021 ttttcacgac gtggccaacg cagtggagat cccgattacg atttttcact atccggcttc
     7081 gaccgcgtcc ggctatttgc cggacacggc gattcggatc gcccgcgaag tcgacagtgt
     7141 cgtcgccatc aaagtggcga gcggcgccaa catcaggcgc tatgagcaag tcctgcgcgg
     7201 catgcgcgcg ttgccgcggc aaatctcggt tctcgcgacc tcttctttat tccagcactt
     7261 cattaccggc gccgatggcg cattgaccgg gtttgccaat ttcgcgccgc agttctgtgt
     7321 cgatctctac aacgcggtca aggcaggcga tctcgacaaa gcgcgccggt tgcacgaaat
     7381 caattgggcg ctggaagccg cggtctacaa ggcgccgaat gtttacaagc acagccgcta
     7441 caaggtcgcg gcttattttg ccggcctgct ggacaatatg atcgtgcgcg cgccgcaagt
     7501 gccggtcccc gagggcgagg taaaactgat tcatgacgcc atggaaaagc tggcaatgct
     7561 caagcgatga ggcggatccg gattccaccg aaggaaggca atgaataagt tcattagtat
     7621 cgttctagtc gtttgcgccg ttataccggc aagcggattt gcccaggaac ggcggaagat
     7681 cagaatctcc aatgctaatt tgagttttac ggctttgcca ttggttgcgg cgcgggaatg
     7741 gaagctcttc gaccaacaag gactcgatat cgaagtgatc ctgatgcgct catcggccgc
     7801 tgctgccgct ttggcatccg gtgacttgga ttaccaatcc ggtatcggga ctgcgtccgt
     7861 cagcgcgacc ctgagcggcc tcgatgcgcg agcgctgtgg gcttcgacaa acaggattgt
     7921 ttactggttg atggctaacc cccaatacaa aagcgtgcgg gatctgcgcc agaagaaaat
     7981 cggtgtgacg ggtctcggcg gcaccagtca tgttgcactg accgcggcgc tcgaaaagag
     8041 cgggctcggc gcgaaggatt atgtcgccgt atcggtgcct tcggcacaat tggtccaggc
     8101 cttggaatcc aagtttgtcg atgcggcgtc gcttaatccg ccgtttatgt tttttgctca
     8161 gcgccgcggt ctagccaagc tcttggacat cggcgctttg gtagaaatgc cttcgggcgg
     8221 ccttaccgct ctgtccaaaa cgatgaaagc ccgaccggat gaagtgaagc gggtggtgcg
     8281 agcgttgcaa acggccaaga gtatgatgct taagtcgcgc gagcgaacct tggacctgat
     8341 tacgggcgtg ctcaggatgg atcgagagac agccgcggag acttacaaat tggtcgaatc
     8401 gaacttcaac gatacgggaa tcccttcacc cgagggcatg gcgaatatcg tcaaggcgat
     8461 catggccgag ggccgcttca cggatagaaa tgtttccttt gaagaagttg ccgagccgcg
     8521 cttcgccatc gaggtcgcta aggagctagg atacaaagta ccgtaggctt cagagcggct
     8581 agctgccgag aggcgcgaac aattcaccaa gcggcggccg ttcgggaata taaccctgct
     8641 cgtgcgcgta ctggacgaat ttatcgagca cgacttgatt ctgcgcggtt aacccgtgcg
     8701 accagggatt atcgccgaag acttcctcct cttcctccag gataagcacc gcgctgggga
     8761 aggccaaacg cttggcatat tcgtaagatt tccggcagcg ttcctcggct tcattaaaag
     8821 catcgagaag gctagagaca agccagggat tttcatcgaa gagcgtttgg cgcaccacca
     8881 aggtgtgcgt gattggaaaa atttttgtcc tacggaaata ttcgtttacg atggcgcgcg
     8941 gatctttgaa gagccggcga atgcgagcat ccttggctcg aaaagagggg acgatgttcg
     9001 gcggaataag cgcgtctatt tcacccttca gcagcatagc ttctgcgggc tggtgagtga
     9061 ccgtgatttt aacgttgggc gaaggctggt atccggcgcc ctcgcgctcg gatgtgaacc
     9121 attcgcattc ctcggggctc acgccataat cttcttgaag aatgcctcgg gcccacagac
     9181 cgaccgtcag acggtactga tccatgccga tttttttgcc tttcaaatcc tccggtcgct
     9241 cgattgccga tgatggtgag caaaaaatat acggatgaat ctgcatgcgc agcgggaaga
     9301 tcggcaatgc gatcaccggc tccttgcggc agcgcgccat catataccag gacagtgaca
     9361 tttcggtgat gtcgaactcc tcgtaaaccg ggcgcatgca cgcctcgccg gtttccgcgc
     9421 gccgcggctg cagcttgatg ccgcgggccg tgacgtcacc gtcgagaata ggacgtgtgc
     9481 ggtgataatc ggccagcagc agagtcaatt cgagatcacg cgccatgata aactccctat
     9541 ggtctcagcg gctctggccg agggccttgg tcaaaaattg tcgggtgaaa aacttttccg
     9601 gcccgccaaa tttttttgct tcggcttcga tgcgcgtcgc gttaatgagg gcgtcgatat
     9661 cttttcttga gatcgaagtc gagtaaagat cggcgaactg gttgatcact cgccggacca
     9721 ttgtcggatc gccgaaagta ttcttgttga tgaccgcttc cgcgaccttt tcgcggtttt
     9781 ttttgagaaa ttcgagtcca cgattgattg cgcgcagtag cgcaacgatg ccttcgggat
     9841 cgttgtcgat cttttgtttg ccggatgcga tgaccacgaa cggaaagccg ggtatggcct
     9901 cgccgatgtc gaaaatggcc ggaaaccctt ttttctcgag ctccaacctt tccaagaaag
     9961 agaacggcga ggcgtgaatt ttgccgattt ccaacgcctg cgcgcgggcg ggactgttgc
    10021 cgacaccgta aagaattttg taatcccggt cgcgcaccat gcctttcttg gcgaggcctt
    10081 cgacagccgc aaattctgca acgccacccg gaccggtgac cccgaccacc ttgcccctga
    10141 ggtcgttaac ggtgctgagc cccttgccgc cgatcagcgt gtaatccata tagggttgca
    10201 tgccgctgac gatgagcatc ggcgcgtttt gcgcgatagc gcgaaccgcc gctactcccg
    10261 caacaaaagt gtaatccacc ccgccgccca ccagcgcggc cattgcggtg gggccgcctc
    10321 gaataaatac cgtttttact tccaaacctt cggcggcaaa aaatccgaag tcctgcgcca
    10381 cgacaaccgg cagcgtcccg ctcaaggtcg gggtgttgta ggcaaaatta acgtttttag
    10441 ccgaaacccg agccggcaaa aacagggcgc tcaacatcag acagtaagcc gacaccgcca
    10501 tctttttggc cgaaaggagg ggcataacct atttaccaag gctttctaaa aacttttctt
    10561 gtttcagtcg atcgacgatg gaactgtcga cgaattcttt gggatcgcgg cggccatgtt
    10621 cgggattttc cgttgccagg ttatcgagaa tgaggcgcac gccgtcaagc gtggggtagg
    10681 gcaagtccgg gaaaagcgcc acgttgaagt tgtattcgat cgcaacaaga ttcggatccg
    10741 tgagtttcat gtacttggaa attatccgct cgctctccgg ccggttgcgt ttcataaaag
    10801 acactgcttc ggtgacggct ttgacatagc gataggccag ctctcgattt gattcgagcc
    10861 aagtttcatg caccaccact gaggtccacg gaaacggaat ggccatttgt ttagccgtgg
    10921 ccaagatcgt caatccttgc ttccgtgcct gctcgctagc cgctgcaccg agaaacgtgt
    10981 actgaacctt gccggttgtc aggccggtca aacggccgct gtcgccgccg agttgcagta
    11041 gcttgagatc gctttcgctc aacccgtttt tgcggaacag atagcgcagc ccttgatgcg
    11101 aagaggatgc aaagcgagaa accgcgccta ctttcccctt tagatcggcg atgctcttga
    11161 tatccggatg gctcaccaag ttggaggcga gcaccgtcag cggattggcg atggtcacta
    11221 acttcgggcc gcgcagccgc gcttccaggg cagcgggacc gccggcggcg aactgaaccg
    11281 tattaccgac gagcgcttgt acgttcaatg agccgctgct gataaacacc aaatcggctt
    11341 cgatcccgta cttggcaaaa aacccccggt cctgtgcaac ccagagcggc gactggctgg
    11401 cattcaccgc cgcataggat gccttgactc gatcggcggc gcccactgct ggcccaaaga
    11461 gaaaaaccgc cagcaacaaa agtgtcgtta acccgagaaa ttgtttcata ctcccgggtg
    11521 aatactgtga agcggtaggg gagtcaagca gcgataaagg cgatgcgcgt acggatcttt
    11581 gtgacgcgct gttcgtcgct atgttcttcg aatgccggtg cggcgaacct cgtatgtaca
    11641 tcgaagtctc tgcgtcgcgg tttcgatata tttttcgcgc aacagtcact ctctttcggc
    11701 ggagtgtcgc gcctgggcca ccgacgtttc agtgttggga cgttggaaat gcattcgtat
    11761 tagttataac cgaaattgca cgcatgattt cgctggcacg tgcattgcgg aatcgtcgcg
    11821 caggattgtt tttatgtcag cccgacaggt ctggcggata gtcaaaggag ggtttatgag
    11881 cagcttaaac aatcagaagg gtgcaatc
//
LOCUS       DASUVS010000137        11049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1797975, whole genome shotgun sequence.
ACCESSION   DASUVS010000137 DASUVS010000000
VERSION     DASUVS010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11049
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797975"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..992)
                     /locus_tag="VF182_07850"
     CDS             complement(96..992)
                     /locus_tag="VF182_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6437029.1"
                     /translation="MTYCYPPDPNTKKPHFIPPAEACDTHFHVFGPPEKFPFVSTHEY
                     TPPAAPLEHYLKMIAIIGIERAVVVQPSVHGLDNSATLDAIANSGGRFRGVGRIDDKT
                     SKAELQRLNDGGIRGVRFNLLARPRGNVELEIFDRCIEHIVQLGWSVDLHIDAKNLLE
                     QEKRIRALPLPVVIDHIARIKPTEGSNQPAFQLLLDLLKEKHVWVKVSGADKICNSRV
                     HGYFGLPFVEVVPYARAVIAAAPDRVIWGTDWPHSNNFAPGHTPNDGDLVDLLAEFAP
                     DEQTRRKILVANPAKLYGFKPS"
     gene            complement(1014..1895)
                     /locus_tag="VF182_07855"
     CDS             complement(1014..1895)
                     /locus_tag="VF182_07855"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6437030.1"
                     /translation="MTETGYRGFRLEEMEYQYNPRESVPEYPQLAKKRAEEARRVRAG
                     AKSWLGVPYGNSPREKLDIYPADRPGGPVLIYIHGGYWRSGNKEDNCNFVPVFTKRGA
                     SVVLVEYDLCPSVTLSDIVRQTRSAITWVYRNILRYSGNPSKIYLSGHSAGGHLTAMA
                     LAYDWEKEGLPRDLIKGAVATSGVYDLDMVMRISVQEQVRLTPELAQENNPFRHPPLP
                     ICPLIIAVGGAEPKGWQQMSEDLFKLCKARGLDCEYLIIPGVNHYTMSEHLADGESPL
                     AQAMLRQMDLHSARALK"
     gene            2291..2590
                     /locus_tag="VF182_07860"
     CDS             2291..2590
                     /locus_tag="VF182_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437031.1"
                     /translation="MKTISLLTNLIISSTLFWGVQTAGGQELMLNVPAEITSYCHMKF
                     PPISEESLFSGSPVLNESAGSSIDFYGSCDHDPLGVDEIRSQRQLLLRGYFADGE"
     gene            2898..5141
                     /locus_tag="VF182_07865"
     CDS             2898..5141
                     /locus_tag="VF182_07865"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6437032.1"
                     /translation="MVRLRQAAYFGVAIGIFGVLLSFVSWVNKSEENLGLAVLFNMRG
                     VRKPPSEAVVVSIDKESSESLSVSGNPARWDRSIHAQLVEKLASRGALAIVFDVYFIE
                     PRSSSEDRLLAVAIKKAANVVLAEPLRAKELSAAGPVRSTDGEHRLVRAIKPIDILSE
                     TATATAPFVLPRLPVRVNYYWTFQPDAGDLPTFPVAAFHIYATEAYPEFRRLLEKLSP
                     DQAARLPTNIEAAKKNGIVTLIKAIRQIFESRPWLYQSMLAELESLDTVEAEPKKYSL
                     IRSLIELHGGSPSRYLNYYGPPRTITTIPFHRAIHLGDDPSGEELDLNGKVVFVGMSE
                     TLTSEREDSFYTVFSQANGVFLSGVEIAATAFSNLLANDHIKPVGSRDYFLTILVWGI
                     LVGLICRITSTLAAALWMVGLSVLFLLAAEYQFQSDGRWYPIVVPLILQSPMAYFGSV
                     FWNLVETNKERQNVRKALGYYVPADMVDLIAKNRLDLRRGGQTVYGVCLLTDIAGYTS
                     VSENLGPRELSELMHGYFEAVFEPIRKNGGLITDLKGDSILAIWKGTRPEAALGRQAC
                     TAALGIAHAVSQFNKSSGWRLPTRIGIHAGSMFLGNIGAGDHYEYGPTGDTVNTASRL
                     DNLNKMLGTGILVSEDAICGLDTFVIRKAGTFRLKGKTQPLVVYELLCQQGDAAEQQE
                     KGCTAFLDALHAFGRQSWHEAEEKFHHCSDMWRTDGLSEFYIRRCQAYIKTPPLDWDG
                     VIPLEEK"
     gene            5192..8581
                     /locus_tag="VF182_07870"
     CDS             5192..8581
                     /locus_tag="VF182_07870"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6437033.1"
                     /translation="MKAAESPRIALVAMIFVVICSYPKISAAAVSCDPWIAKMVSVQG
                     NVQVRRAGQTQWQQARLNDVYCAGDRIQVGERSRADVALANQPILRLDQNSTINLGGI
                     KEQRTAWVELLTGALYFFSRLPRNLEIQTPFVNAGVEGTEGFVLVEATRTAVSIFEGT
                     VLTSNQQGKLTVTTGQTAITEQGRAPTLTATIRPDDAVGWALYYLPTLYFRLQEFPAG
                     SDWRGMVRESLEAYIRGDLQTAFNSVQAVPATLSEPRFFAYRAALLLAVGRVDEANQD
                     IERALGLDPNDSNAFSLQSIIAVVQNEKEKALALGQRAVTADPKSAAARISLSYAQQA
                     NFDLDGALKSLQQATQLDPENALAWARLSELWLSFGRLDEALAAAGRAVAIDPNLSRT
                     QTVLGFAFLTRVDTEEAKKAFERAIGLDQADPLPRLGLGLAKIREGDLQAGRMEIEIA
                     VSLDPQNALTRSYLGKAYFEEKRDKQAADQYELSKPLDPKDPTPYFYDAILKQTTNRP
                     VEALQDMEKAIELNDNRAVYRSRLLLDADLAARSAALSRIYSDLGFQQLALVEGWKSV
                     NTDPTNFSAHRFLADSYSALPRHEIARVSELLQSQLLQPLNMTPIQPHLAESNLFLIA
                     AQGPAALSFNEFHPLFSRNGVTFQTTGLAGENKTYAGEGVLSGIYNKAAYSLGGFHFQ
                     TDGFRKNANQRDTIGNAFGQFEFSPQTSVQAEYRYRNLVRGDIRLRFFPDDVFPDETN
                     EEQTQTARLGLRHAFSPNSIVLGSVIYQDADFASREDEIPQSPFFTFSDAKRPETAFS
                     AELQHRFRARYFNLVSGIGHANSDGKVDITAGTIFPPPSDIITFPRVSTDLRQTNVYA
                     YSYINALREMTFTLGGSGDFADGDGPEFKSKRQFNPKLGTTWNPFPATTVRAAVFRVL
                     KRKFITNQTIEPTQVAGFNQFFDDNNGTDAWRYGAAIDQKFTEKFFGGIEFSKRDIES
                     PFVVNSTTGRVQDMDERMARTYILLTPHPWVALSAEYLFERFKSEGLDAQPEKLHTHR
                     APFGISFFHPSGLSAGLKATYFNQDGTFALINQAARSGHEDFWTVDAAINYRLPNRYG
                     FATIGATNLFDKNFKFFDRDVRNPMIQPDRTFFARLTLALP"
     gene            complement(9749..10048)
                     /locus_tag="VF182_07875"
     CDS             complement(9749..10048)
                     /locus_tag="VF182_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437034.1"
                     /translation="MKKTISLLTNLLLSSTLFWGVPTAEGQEGIVRIPAETTSYCHMT
                     YPTIREDALLWLDPWLNESAGNIVDFYGSCDHNLLSVDEITQRRVVRRGIYGDAE"
     gene            complement(10342..>11049)
                     /locus_tag="VF182_07880"
     CDS             complement(10342..>11049)
                     /locus_tag="VF182_07880"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6437035.1"
                     /translation="AQAGTLFLDEIGDLSLPSQAKILRVLQERSVTRVGGRTAVPLDL
                     RIIAATNKDLEHEIKDGHFRVDLYYRLKVIHIETPALREVPEDIPLLANHFLGKYSKL
                     MKTEPKQFTPAALECLASYAWPGNERQLENEMRRLVASIRGKTISDEHIDPAIRRPEE
                     TLASSTIPSSFKTPQVENTPAISRSPKSLPDAVEALERRMIAEALRESGGNKQKAAQF
                     LGLSRQGLIKKLKRLEI"
BASE COUNT         2578 a         2822 c         2913 g         2736 t
ORIGIN      
        1 gctgaacctt ttcagctagt ctaccgtcag gcaaaccgca tctttgtctg tttgcgtccg
       61 gacagcctgc aatcagattt ttcaaagtgc cgcctttaag agggtttgaa tccatataac
      121 tttgccggat tggcgacaag aatttttctt cttgtttgct cgtcgggcgc gaattcagcc
      181 agcaaatcca ccaagtcgcc gtcattgggt gtatggcccg gcgcgaaatt gttggaatgt
      241 ggccagtcgg taccccagat cacccgatcc ggcgccgcgg cgatcaccgc gcgcgcatac
      301 ggaaccactt caacaaacgg caacccgaaa tagccgtgaa ctcggctatt acagatcttg
      361 tcggcgccgc tgacctttac ccagacatgt ttctctttca atagatcgag cagcagttga
      421 aaagccggct gattcgaacc ttcggtcggt ttgatgcgcg cgatatggtc gatgacaact
      481 ggcaggggca gtgcgcgaat gcgcttttcc tgttcgagta agttcttggc atcgatgtgc
      541 agatccactg accagcccaa ttgaactatg tgctcgatgc agcgatcgaa gatttccagc
      601 tctacgttgc cgcgcggacg ggcgagcaaa ttgaaacgca ccccgcgtat gccgccgtca
      661 ttcaatcttt gtagctcggc tttcgatgtt ttgtcatcga tccggccgac gccgcggaag
      721 cgtccgcccg aattggcgat ggcgtcgagt gtcgctgaat tgtcgagacc gtgcacgctc
      781 ggttgcacga ctacggcccg ctcgataccg ataatggcga tcatttttag ataatgctcc
      841 agtggcgccg ccgggggagt gtactcgtgg gttgagacga agggaaattt ttccggtggg
      901 ccaaatacgt ggaaatgagt atcgcaggct tcagctggcg gaatgaagtg cggcttcttg
      961 gtatttggat ctgggggata gcagtacgtc acgggactct cctgagctaa agctcacttc
     1021 agtgcgcgcg cggagtgcag atccatttgt cgcagcatgg cttgcgcgag tgggctctcg
     1081 ccatcggcca gatgttccga catggtgtag tgattgacgc cgggaatgat gaggtattca
     1141 caatctaagc cgcgcgcttt acaaagtttg aaaagatcct ctgacatttg ctgccagcct
     1201 ttcggctcgg cgcccccgac agcaataatc agcgggcaga ttggcaaggg agggtgtcga
     1261 aacgggttgt tttcttgcgc tagttcgggc gtgagcctga cctgttcctg gacgctgatg
     1321 cgcattacca tatcaagatc atagacaccg gaggtcgcga cggcaccttt gatcaggtct
     1381 ctcggtagac cttctttctc ccagtcgtac gccaacgcca tcgccgtcaa atgtccgccg
     1441 gcggaatgtc cggagagata aattttcgaa ggattaccgc tgtaacgtaa aatattccta
     1501 taaacccagg tgatggcaga gcgcgtctgg cggacgatgt cggagagggt cacgctagga
     1561 cagaggtcgt actcgaccag gactaccgat gcgccgcgtt tggtgaatac cgggacgaag
     1621 ttgcagttat cttctttgtt gccgctgcgc caatagccgc catgaatata gatcagaacc
     1681 gggccaccgg gtcgatctgc gggatatatg tccagcttct cacgcggcga attgccgtaa
     1741 ggcactccca gccaagattt tgcgcctgcg cgcacccggc gcgcttcttc ggcgcgcttt
     1801 ttcgccagct gcggatactc cggcactgat tcgcgcggat tatattgata ttccatttct
     1861 tcaaggcgaa atcctctgta gccggtctca gtcatgtgta tccttccgtg gatctgattg
     1921 gcactgtgtc ctccgacgaa cgtaatttac tagggtcttt aatgtcaact gccgctgact
     1981 ctgtgaacat ccgatcagtc ctgcgcaacg acgtctgcga ggtaatcgtc aattgaaatg
     2041 gacgataagg aaccggtcta gatgtaaact atgttgctag ttttgtttcg ataccatcta
     2101 acggacactc tgcggtgtcc aaaagtatca cccagaaatt tgacaggcag tcatttcaaa
     2161 cgttcattgc ttgaaactcg agtgaactgg ccggttacca tgcagacaaa agtcttcagg
     2221 caaatggcac aaccgatgca atgaaggctt cgcaggaagt ggaaacttga acagaaagga
     2281 ggtgagaaag atgaaaacca tatcgcttct aaccaacttg attatctctt cgaccttgtt
     2341 ctggggagtg cagacagcgg gcggccagga actaatgctc aatgtgcctg ccgaaatcac
     2401 cagctactgc catatgaaat ttcccccgat cagcgaagag tcactattct ccggcagccc
     2461 ggtgctgaac gagagcgccg gcagcagcat cgatttctat ggctcgtgcg atcacgatcc
     2521 gctaggagta gatgagatcc gctctcagcg acagctgctt ctgcgcgggt acttcgccga
     2581 cggcgagtga accgaagagc gccagttcag gggagggcgt acctcccctg aacgattgaa
     2641 cttgcttaaa aatccgcgca gtactatatc cttgctcact tcttagcttc gggttgcggt
     2701 tcggctattt gggccgcgat atcatagtca gctgtgcgag ctttactctt tcgatcgctt
     2761 tggatgaaca tagctggggc gcttaatgcg cacctaagag cacagatcta aatcctaaac
     2821 ggggaacgga cccacagagt cctgcacgtc ctgttccgga gtacggttca agatttaacc
     2881 ctacagcgga ttcgcaaatg gtccgacttc gtcaagcagc atattttggc gtcgcgattg
     2941 ggatattcgg cgtgcttcta agcttcgttt cttgggtaaa caaaagcgag gaaaacttag
     3001 gtctggctgt gttgtttaat atgaggggcg tgagaaagcc accgtccgaa gctgtagtcg
     3061 tgagcatcga caaagagtcc tcggagtctc tgagcgtctc cggaaatccg gcgcgatggg
     3121 atcgttcgat ccatgctcag ttggtcgaaa agctggcgag ccggggtgcg ctcgccatcg
     3181 tctttgacgt ctatttcata gaaccgagat cgtcatccga ggacaggttg cttgcggtgg
     3241 caataaagaa agccgcgaat gttgtacttg ccgagcccct aagggcaaag gaactttccg
     3301 ctgctggtcc agtccgctct actgacggtg aacaccgcct ggtcagagcc attaaaccca
     3361 tcgatattct ttcggaaact gccactgcga cggccccttt tgtccttccg aggctaccgg
     3421 tcagagtaaa ctactactgg acctttcaac cggacgcggg agatttgccg acttttccgg
     3481 tggcggcgtt tcacatctat gccacagaag catacccaga gttccgccgg ttgttggaaa
     3541 agctcagtcc ggatcaagcg gcaagacttc caaccaacat cgaggcggcg aagaagaacg
     3601 gcattgtgac gctcatcaaa gcgattaggc agatttttga aagccggccg tggctgtacc
     3661 agagcatgtt agcagagctg gaaagcctgg atactgtaga ggcggaacct aagaagtatt
     3721 ctttgataag atctctgata gaactccacg gcggcagccc tagtcgatat ctgaattact
     3781 acggtcctcc gcgaaccatc accacgattc catttcatcg ggcaatccac ctcggcgacg
     3841 acccaagcgg cgaggagcta gacctcaacg gtaaagtcgt tttcgtcggg atgtcagaaa
     3901 cattgacttc agaaagagaa gacagctttt atacagtctt ttcgcaagcc aacggtgtgt
     3961 ttctcagtgg tgtggagatc gccgcaaccg ccttctctaa cttgctcgcc aacgaccata
     4021 tcaaaccggt cggttcacga gattactttc tcacgattct cgtttggggc attcttgttg
     4081 gactgatttg tcgaatcacg agcacgctcg ccgccgcgct ttggatggtg ggtttgagtg
     4141 ttttgtttct tctcgctgcc gagtatcagt ttcagagtga cggcaggtgg tatccgattg
     4201 tggttccgct aattttgcaa tctcctatgg cttattttgg atcggtgttc tggaatctag
     4261 ttgagacaaa taaggagcga cagaacgtca ggaaggctct cgggtactat gtccccgccg
     4321 acatggtcga tctaatcgcc aagaatagac ttgacttacg gcgaggcggt cagacggtct
     4381 atggcgtctg tctgcttacg gacatagccg gctacaccag tgtgtcggaa aatctaggcc
     4441 cgcgcgaatt aagtgaattg atgcacggat acttcgaggc agtgttcgag ccgatcagaa
     4501 agaacggcgg cttgattact gatctaaaag gcgactcgat tctcgcgata tggaagggga
     4561 ctcgccctga ggctgcttta ggtcgtcaag catgtacggc tgctctcggc attgcccacg
     4621 ccgtcagtca gtttaacaag tcgtccggtt ggagacttcc cactcgcatc gggattcatg
     4681 ccggatcgat gtttctgggc aacattggcg ctggagatca ctacgagtac ggtccgacag
     4741 gagacacagt caatactgcc tcccgactgg acaatctcaa taaaatgctg ggcacgggaa
     4801 ttcttgtctc ggaagatgcg atttgcgggc ttgatacatt tgttatcaga aaagccggga
     4861 cgtttcggct aaagggaaag acccaaccgc tcgttgttta tgaactactt tgccagcaag
     4921 gcgatgctgc ggaacagcaa gagaaaggtt gcacagcatt cctcgacgca ctccacgcgt
     4981 ttggtcggca atcgtggcat gaggccgaag aaaagtttca ccactgcagt gatatgtggc
     5041 gaaccgacgg cttgtccgag ttctacataa gacgatgtca ggcgtatata aaaacgccgc
     5101 ctctcgattg ggacggtgtg attccattag aagagaaatg atagttttgc tcgtaagtgg
     5161 caactgatcc gacgatgaaa gcaggtaagc catgaaagct gccgaatcac cccgaatagc
     5221 gctcgttgcc atgatcttcg tcgtcatctg cagctatccc aagatctctg ccgcagcagt
     5281 ctcatgcgat ccttggattg ccaagatggt atcggtgcag ggaaatgtgc aagtgaggcg
     5341 cgcgggacaa actcagtggc agcaggctcg cttaaacgac gtttattgcg ccggtgaccg
     5401 catccaggtt ggggaaagaa gtcgcgccga cgtcgccctg gccaatcaac cgattttgcg
     5461 tctcgatcaa aatagcacca tcaatttggg gggaataaag gagcaaagga ctgcatgggt
     5521 agagctactg accggcgctc tgtacttttt tagccggctt cctcgaaatc tggagataca
     5581 gacaccattc gtcaatgcag gggtggaggg aaccgaaggc tttgtgctcg ttgaagcaac
     5641 tcgaacggct gtcagcatct tcgaaggcac ggtgctaacc tcgaaccaac agggtaagtt
     5701 gactgttaca actggtcaga cggcgataac tgaacaaggc agagcgccga cattaacggc
     5761 taccattcga cctgatgatg cggttggctg ggcactgtac tatttgccca ctctctattt
     5821 ccgcctgcag gaatttcccg ccggttcgga ctggcgggga atggttcgag agtcgctcga
     5881 ggcttacatc agaggtgatt tgcaaaccgc cttcaacagc gttcaagcag tcccggcgac
     5941 gctaagcgaa ccgcgcttct ttgcttaccg tgccgcgttg ctgttggccg tcgggcgggt
     6001 ggatgaggca aatcaagata ttgagcgcgc actaggtttg gacccgaatg acagtaatgc
     6061 cttctcgctt cagtcgatta ttgcagtggt ccaaaatgaa aaggagaaag cccttgctct
     6121 ggggcaaaga gctgtcacgg ctgatcccaa atcggctgcc gcacgaatct cgctttccta
     6181 cgcccagcag gccaatttcg atctcgatgg cgcgttgaag agcctgcaac aagcaaccca
     6241 attagatcct gaaaatgccc ttgcatgggc gcggctttct gaactttggc tgtcctttgg
     6301 ccgcctcgat gaagctctgg cggcggctgg acgagctgtt gcaatagatc cgaatttatc
     6361 aagaacgcag acggttttag gctttgcctt tttaaccagg gtcgataccg aagaggccaa
     6421 gaaggctttc gagagggcca tcgggctcga ccaggctgac ccgctccctc gacttggcct
     6481 gggcctggcg aagattcgcg aaggcgatct gcaagcgggg agaatggaaa tagagatagc
     6541 cgttagcctg gaccctcaga atgcgctcac ccgaagctat ttgggtaaag cgtatttcga
     6601 agagaaacga gacaaacaag cggctgatca gtacgagctg tccaagccgt tggacccgaa
     6661 ggatccgacg ccctatttct acgatgccat tctcaagcag accactaatc gtccggtcga
     6721 ggctttacag gacatggaga aagccattga gctcaacgac aatcgcgcgg tttatcggtc
     6781 gcgtctcttg ctcgatgcgg atctcgccgc gcgcagcgcg gcgctaagtc gaatctacag
     6841 cgacttgggt tttcagcagc tcgccctcgt agagggatgg aagtcggtca ataccgaccc
     6901 gacaaatttt tcggcgcacc gatttttagc ggactcctac tccgcgttgc cgcgccacga
     6961 gatcgccagg gtcagcgaac tgctgcagtc gcaactgctt cagccgctca atatgacacc
     7021 gattcagccg catcttgccg aaagcaattt gtttctcatc gcggcgcagg gtccagcagc
     7081 tctctccttc aatgagtttc atccattgtt cagtcgaaac ggcgtgacat ttcagacgac
     7141 cggtctcgcc ggagaaaata aaacctatgc cggcgaagga gtgttgtccg gaatttataa
     7201 taaggcagcc tacagcctcg gtggctttca ctttcaaacg gatggtttcc gaaaaaatgc
     7261 caatcaaagg gatacgatcg gaaacgcatt tggtcagttt gaattttctc cgcagacgag
     7321 tgttcaagcg gagtatcgct atagaaattt ggttcgaggc gacattcgat tgcggttctt
     7381 cccggacgat gtcttccctg acgagacaaa cgaagaacaa acgcagaccg cacgcttggg
     7441 tttgcggcat gcgttttcgc cgaattctat cgttctcggc tcagtcattt atcaagatgc
     7501 cgattttgct tcgagagaag atgagattcc tcagagtccg tttttcacat ttagcgatgc
     7561 aaagaggcca gaaactgctt ttagtgccga gctgcaacac cgcttccgtg cccgatactt
     7621 taatctcgtg agcggaatcg gccatgctaa ttctgacggg aaagttgaca ttaccgcggg
     7681 gacgatcttt ccccctccat ctgacattat aactttccct agagttagta cggatcttag
     7741 acaaacgaat gtttatgcat actcttatat caacgccttg agggagatga catttacact
     7801 gggggggagc ggagatttcg ccgacggtga cggtccggaa ttcaagagca aacgtcaatt
     7861 caacccgaaa ctcgggacta cgtggaatcc atttccagcg accacggttc gcgccgccgt
     7921 attcagggtt ttgaagagaa aatttatcac gaaccaaacc attgagccga ctcaagtcgc
     7981 cggcttcaat cagttttttg atgacaataa tggaacggat gcgtggcgct acggcgcggc
     8041 tatcgatcaa aaattcacgg aaaaattctt cggcggcatc gagttctcca agcgcgatat
     8101 cgagagtcca ttcgttgtta atagcacgac aggtcgagtg caggacatgg atgaacgtat
     8161 ggcgcgaacc tatattttgt tgacaccgca tccttgggtc gcgctcagcg cagaatatct
     8221 gtttgagcgt tttaaaagtg agggattaga tgctcagccg gagaagcttc acacccacag
     8281 ggcgcctttc ggaatcagct ttttccatcc ttcgggacta agcgctgggc tgaaagccac
     8341 ttactttaac caagacggca catttgctct tattaaccag gctgcccggt ccgggcatga
     8401 agacttctgg acagtggatg cggcgataaa ttatcgtctg ccgaaccggt acggttttgc
     8461 aactatcggt gcgactaatc tttttgacaa aaacttcaag ttttttgata gggacgttag
     8521 aaatccgatg attcaaccgg accggacatt tttcgcaaga ttgacattag ccctgccgta
     8581 ggattagtcg gtccatcgaa gcaactgctt tccgttggaa accgaggcat cacttgcacg
     8641 tcaagaagta atcgactacc gccgcgtgag aagggtgccg attccaatgg agagcggacc
     8701 gtctggatct cttgaatgct gttcgctttg atgtaccaat acggccgtac gtctgtcctc
     8761 aaggcatgct gaacgatgag acaaacgatg aatagctgct cagcctatgg acaaatcgta
     8821 tatgagcccc cacctggcag atttaaggta atacagttgg agcccgtata ggcgagtaaa
     8881 gttccgatat atctatatcc gcccggagga tttgatggat gcttgctctg gtcccctaag
     8941 ctaacgatct ccccttccga ttccggtttg atttctgtta ctttttggac tctttctatt
     9001 ctacccgtac ctacgtccac cgtaataagc atcttatatt ctccaccgtt tgtcatattt
     9061 tcctccgttc tgccttttac ttttaattgt aaaaaagtca cagccttaat ctttagactt
     9121 ctccttaaac acggctatgg cgttacctta aggtcgaagc aggttagcga ggagagagaa
     9181 gcaacccatg ctacacacac aactgatatg acccaccgcc tgggaggttt aaagtgatgc
     9241 agttggaccc cgtatacttc atcaatgtgc cgataggtat accctgcggt tgattctccg
     9301 atttctcgat gtcttctggt tgccctgccc ctcttttttg tactttttca attgtaccgg
     9361 tatttacatc cacagtaata accatcttgt tatggagtgg gggtgtggcc atagatcctc
     9421 ctttgtaggt ttaaagtgtt gatacagacc atttatatct caccataaga acttccctct
     9481 agagacatga tcaaagccac taaagagtta cgaaacaagc acgcaccgcc gttcggctgc
     9541 ggtcatctaa aggcactccg gacacaatgg ccgaactcta gtcgccgacc gatttccatg
     9601 tcaagccttt ttttggcatt ttaagatact tttggtaaac ttcatccgga cactcccata
     9661 gaatcagtaa ccgaaatgtt gttgtctccg ctattagggg agagagcgga tgcttcccta
     9721 atgcacattc gctcaatgtt tgacctagtt attccgcatc accataaatc ccgcgtcgca
     9781 caacgcgccg ctgagtgatt tcatcgacgc tcaagagatt gtgatcgcag gagccgtaga
     9841 agtcgacgat attgccggcg ctttcgttga gccatggatc caaccagaga agggcgtctt
     9901 cgcgaatcgt gggatatgtc atatggcaat agctggtggt ctctgcaggg attctgacaa
     9961 tcccctcctg gccctcggcg gtcggcactc cccagaatag cgtcgaagac agcagcaggt
    10021 tcgttaaaag tgatattgtc tttttcattt ttctcatctc ctttcttttt gttttcactt
    10081 cctgcttctt ctaagagcat cagttatgcc agttcggtag agctgaaaat cgttaacgtt
    10141 catcgcgtcg aaaaatctaa aaaaggtaac ggcgttgccg ctagatctac tttgttggtg
    10201 gttgacgagc taacgatgtg gacacatggc tgacagcgtc agatgaattc agaacgttgg
    10261 atggcgtcaa accagcgtgg ctgatctaaa cccacggttg ggtgcgaagg atttccggcg
    10321 gcgaacaggt cacgggcttg attagatctc tagtctcttt aacttcttga tcaatccctg
    10381 acgactcaag ccaaggaatt gcgccgcctt ttgtttattg ccgccggatt cacgtagcgc
    10441 ttcggcgatc atgcgccgct cgagagcctc gacggcgtca ggcaacgact tcggcgacct
    10501 cgatatcgcg ggagtgtttt ccacctgcgg cgttttaaat gaacttggga tcgttgagct
    10561 tgcaagtgtc tcttccggcc tgcgaatcgc cggatcgata tgttcgtctg aaatagtttt
    10621 tcctcgtatt gaagcgacca gccgcctcat ttcgttttct agctgtcgct cgttccccgg
    10681 ccatgcgtag ctggccaggc actccaaagc cgccggtgta aactgctttg gctcggtctt
    10741 catcaattta ctgtatttcc caaggaaatg gtttgcgagg agcggaatgt cttcaggtac
    10801 ttcccgcaaa gccggagttt cgatgtggat cactttcaaa cggtagtaaa gatcaacccg
    10861 aaaatgcccg tccttgattt catgttccag atctttgttg gtggcggcga taatgcgcag
    10921 atctaggggc acggccgttc ggccgccgac ccgcgtcacc gatcgttctt ggaggacgcg
    10981 caggatcttg gcctgtgacg ggaggctgag atcgccgatt tcgtccaaga atagcgtgcc
    11041 ggcctgagc
//
LOCUS       DASUVS010000138        18056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_268961, whole genome shotgun sequence.
ACCESSION   DASUVS010000138 DASUVS010000000
VERSION     DASUVS010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 18056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18056
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268961"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..782)
                     /locus_tag="VF182_07885"
     CDS             complement(297..782)
                     /locus_tag="VF182_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437036.1"
                     /translation="MGNRQIYISYRLVSVALKYPGQLWLLRQTVAHEIAHETAGHAKG
                     KGIKQVNSGFFNFAGSGSDLGLPWYVRLYNYSADRELEADGIGLGYWNKLGWDCRIWV
                     RILENFQEQNYIGDRFHPTDKRLQQAQNVCKLHGDEKPSAQSARPAENGKFAFVNSEL
                     N"
     gene            complement(1037..1405)
                     /locus_tag="VF182_07890"
     CDS             complement(1037..1405)
                     /locus_tag="VF182_07890"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEX6437037.1"
                     /translation="MPFTIIDGTGPIISAKISGELDKSEVTQIQTAALQAILRCGKVS
                     ALLILENFSGWKRGADWGDISFMSEHDKDIAKIAVVGEDKWRDSIYAFLAKGFREAEI
                     EFFLPGDLEKARVWLSANTP"
     gene            complement(1542..1997)
                     /locus_tag="VF182_07895"
     CDS             complement(1542..1997)
                     /locus_tag="VF182_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437038.1"
                     /translation="MHRVLLGWLIVGTSLLLPQLSFAATQAADGALKITTRTVAPGTG
                     LSWGEGLLSYKGQDYPLTFEARGLFRNVDAEITAAELSGQVFNLKSPEDIAGNYQMAE
                     AKEAESGAGSSATIKNEKGVVINLVSTVAGRKFNLAFSGLDVELKKPKP"
     gene            complement(2359..2682)
                     /locus_tag="VF182_07900"
     CDS             complement(2359..2682)
                     /locus_tag="VF182_07900"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEX6437039.1"
                     /translation="MTNTSAIILALVFFGCSEPLTTREKGAVVGTVGGAGLGAIIGSA
                     TGNAGAGAGIGAAVGLLGGALIGDQMQARQQQDADVQRQIQAQQAEIDRQQRELDRLK
                     AQQAR"
     gene            complement(2747..3190)
                     /locus_tag="VF182_07905"
     CDS             complement(2747..3190)
                     /locus_tag="VF182_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437040.1"
                     /translation="MKQNVLRSAGFLLALALVMIAAGFNLYAMDSNNRYFAYGVGQRS
                     CEDYVKFREKRLDTLEQQHPRYTKDELYEIVDRIVEHWIAGFLTAHNFYVSDTYDVAG
                     KSNMADLKDRLEKSCQTKTKQHFAEAMLTVIQQLNPQRVKVGTGK"
     gene            complement(3306..4304)
                     /locus_tag="VF182_07910"
     CDS             complement(3306..4304)
                     /locus_tag="VF182_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEX6437041.1"
                     /translation="MRIIGWFVVGLVVGALTLWAVGMLYYSPLLPEKARAIGAAGFAV
                     ITILAFLFLPRRGRTLLGFVAVLAILVVLFLRIPASNDRDWQPEVEMTPYATINGDTI
                     TIHNLRNFEYRTETDFTPRWETRTYDLQKLDSVDLIAVYWAGKAIAHIMVSFGFAGKD
                     YLAVSIETRKEKNESYSTLAGFFRQYELYYVVADERDVVRVRTTYRNPQEDVYVYRVN
                     GPIQNVRRVFLDYIKSINDLRDRPSYYNTLTTNCTTSILMHTRMNPESPPMSWKILVS
                     GYVPDYVYELGRLDTSRSFADLEKVSHVNQRAHAADKDPAFSQRIREGLPTPAAKP"
     gene            complement(4423..6297)
                     /locus_tag="VF182_07915"
     CDS             complement(4423..6297)
                     /locus_tag="VF182_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6437042.1"
                     /translation="MLVEAKKKRALLAISLRGLFCCIVTLQLLASGCATPIGVNYVNP
                     RIAYQSLTGNILSAERPSSFSARELMNLNLYQRFQDDPAKALSEMHAGLAPKGDEDRL
                     FALVELSFAYAEQTEDKSYYLAAAVYAYSFILPGEHGTAPRAIDPRFRWAADIYNQAL
                     TRAAMIDQKPVPRAGKFSLPFGEIDIDFNEDDLIWAGYRLTGFVPAADIAIRGLRNRY
                     RIPGIGAALAASIEPLAETASKEHARIPPRLRVPVTAFLRLDDPRGALGSGKLKGKLE
                     FYTPYSARTVSIAGIDVPIEYETTSALALTLEGAPIWDFEIAGFRSGDFNIGEGNLRQ
                     GLFMLHPHRPGRTPLVLVHGTASSPARWAELVNELENDRRFWENYEVWLFMYNTGNPI
                     AYSALLLRDALTDLVSELDPTGTDAGLKNMVVMGHSQGGLLTKMSVIDSGMALWPFSV
                     SPEELDVSAETRDLLTRALIFKPLPFVKEVIFVATPHGGSFQALGLLGNFASWLVNLP
                     GRFTKLSFDLVTLQKQGFVLGPFSGMPTSITNMNPGNRFIKALHGIPIANGVVAHSII
                     AVEGDGPPEQGDDGVVKYSSAHIEGVVSEKVVRSSHSTQGHPETIQEVKRILFERKKE
                     "
     gene            complement(6310..6528)
                     /locus_tag="VF182_07920"
     CDS             complement(6310..6528)
                     /locus_tag="VF182_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437043.1"
                     /translation="MSAAQDEGVRIISEDRGSGVIRGVRNDQEVTLNLRSQADGSVRI
                     EIGARGPKGAEPGLASRISRAYDRRMGR"
     gene            complement(7120..8064)
                     /locus_tag="VF182_07925"
     CDS             complement(7120..8064)
                     /locus_tag="VF182_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HEX6437044.1"
                     /translation="MAMPLKQNKTRSTCLRNCFAAIALIFALAISSEAALAQKTFSSA
                     EEAVKAAVAAAKSNNQKELLAIFGTRAKELLFSGDSVADEQRREEFLAAYDEKNRLAT
                     EGENTILIVGKQDWPFPIPIVKKGQSWMFDADKGREEILNRRIGENELSTIQSLLAIV
                     DAEREYAMKDRDQNALLEYAQKFVSDPGKKNGLYWEAQRGEPQSPLGPIMARVRSEGY
                     QVSATPVPYHGYYYRILTAQGKDAPGGAYSYLVKGRMIGGFAVVAYPAEYGNSGVMTF
                     IVNHEGKVFQKNLGPNTASISKGMKEYNPDKSWTEVKD"
     gene            complement(8181..9467)
                     /locus_tag="VF182_07930"
     CDS             complement(8181..9467)
                     /locus_tag="VF182_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HEX6437045.1"
                     /translation="MLAKRLFARGFVWLMVLLLAITPGVIAQQSDTAAVFKPEELEQI
                     LAPIALHPDSLVTQILMAATYPLEVVQADRWIKQNASLKGEALTAALEKQQWDPSVKS
                     LVNFPQVVTMMSEQLEWTQKLGDAFLADQKQVLDTIQSLRAKAQASGNLQTTKEQTVI
                     VEEKIIKIEPANPQVVYVPTYNPTVVYGGWPYPAYPPYTYYPPGYVASSAMWFGAGMA
                     MGAAWGYAWGNSNWNGGEVDIDVDRNTNINTNINRDQAKQKLQERGQGNQRGQGKWQH
                     DPEHRKGVSYRDQGTAQKFNRASSNDAIQSREQFRGRADQGRQDLARTGAGDRGGLSG
                     AGDRGGLGGQGGGGDRAAGLGGGAGGNRAGTMDRGGGGGSGRGGAFQGVSGGGSAAAS
                     ASSRGSASRGSFSGGGGGARGGGGGRGGGGGGGRRR"
     gene            complement(9799..11475)
                     /locus_tag="VF182_07935"
     CDS             complement(9799..11475)
                     /locus_tag="VF182_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437046.1"
                     /translation="MMQSQQFSPFRRNIAFGAMIILSVILVLQPKRGSAAAQTGYESL
                     PVLSASKILPPELVKGPNHQVQERVVNDGFLNTYTIDSRFGQFRAVSTALLRARIQEI
                     NAMAAMDKLKGTTEYGRAVKESTLSAMVAAKDMVFQPVQTVKTAASGVAVAFRRAGDS
                     IFGAKRSDVEDNRFKSLIGFSNYKREYAYDLGVDVYSRNEVLQDRLNEISWVGFAGGL
                     TVSAAMAAVPGTAGTVMTAVGTARLTREIFKNTPPADLRRINMEKLNAMGIDPATVEM
                     FMNESIFSPREQTVLVFALDEMKGIGDAERFVQLAALTNNADMALFRQRQAEMYAGYH
                     KAVSPLSQFVSVGQVPGVRAANGALVFNFPIDHLVWTDSLARLVANTDMLANQLPGVT
                     RKELWLTGNLSPRARNELRSIGWTTYENAEALLVAQDKATPSYRKEGQTPDATVKLKS
                     RSIALGAGVNWGEGVLSYQGKDYPITVSGLSLLDLGVSDVSATGRVYFLKQPSDFSGN
                     YFATQATFAVAGGGGELVMANGNGVFIALQSEQSGTQLTLGASGVSLKFK"
     gene            complement(11559..11942)
                     /locus_tag="VF182_07940"
     CDS             complement(11559..11942)
                     /locus_tag="VF182_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6437047.1"
                     /translation="MKKSKPLIAIVDDDESVCRAVRRLVRSLAMNAQTFSSGQDFLDL
                     LHAMPSFKPDCLILDVQMQGINGLDVQEKLTKGGKTIPVIFITAHDEVGVREKALAAG
                     AVAFLRKPFNDELLIKTMDQAMKYT"
     gene            complement(12455..13072)
                     /locus_tag="VF182_07945"
     CDS             complement(12455..13072)
                     /locus_tag="VF182_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6437048.1"
                     /translation="MFLVDDDASVRRALTRLIKVGGYAVQAFASARDFLEYWQANDQN
                     PACLVLDVRMPGLSGLDLQQTLQAANTLLPIIFITGHGDIPMSVKAMKQGAVDFLPKP
                     VKDKDLLRAIEQALARASHDYTERLELEEIQTRLTSLTPREGEVMTLVVSGLLNKQIA
                     FELGTVEKTVKVHRARVMEKMKVESLAELVRLAEKVGIPAQKNKN"
     gene            complement(13382..16498)
                     /locus_tag="VF182_07950"
     CDS             complement(13382..16498)
                     /locus_tag="VF182_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6437049.1"
                     /translation="MSGSSSYQSLGALRSKTLMILLLAVLFCFTPARSASNAEPAPVK
                     RVLIISVGSRLAPGFVIADQQLLAALRKIQTARIEIDAENLDLVRFPTERYQQVFRDY
                     LSAKYSENRPDLVILVYTGNLGITGTLLPQLFPNTPIIVAGFTEEELNRKQFGKLVSG
                     VAQRVNPQATLDLIGRLQPEVNRVIVIGGTTETDQQVTQRIRDAAEPFKSRLKIEFWD
                     NFRMDELNKTVKVLPANTAILFSRVFRDGAGQALISAEVARTLAQEANAPVYVMTETG
                     LGTGAVGGALASIEAFGQRAGELARLVLTGTAPASLPLEIRTDTVPTFDWRALQRWRI
                     PENRLPANSIVRFRPQSFWQEYRWYIIAALIIIGLQTTMIADLLLQRRRRRKVEAELR
                     DSRELMEHAASAGELGLWSRHLTATEVWINGPGRSLLGFGSNETVTFEAIQERIHADD
                     LKRMLSELKEAQTAGLPFQGEFRIVLPDGTNRWLLAKGRITAESGSGRDRRRMGMVID
                     ISERKRAEQKLRESEESFRRLVETTAAVIWQADSDNWVFTYVGPQATKLLGYPVEQWY
                     EKDFWVSHIHPEDREQAVNTCLTMSQSAKDFNFEYRMIKASGEVVWIHDIVNCQHQNG
                     TGKTQLRGLMLDISARKQAEDAVRSERQFLRQVIDIDPNFIFAKDREGRFTLANKAVA
                     EAYGTTVDNLIGRTDADFNPNQSEVESFRQADLDVIDTLQERFIPEERITNGGGRVRW
                     LQTVKRPIIESDGSAKQVLGVSTDITQRKKTEMELREQRVELAHVSRISTMGELAASL
                     AHELNQPLTAILSNAQAALRFLSSKPADIEEVHEILRDIVEDNRRAGEVIRRMRALVK
                     KEGIEFASIDFTGLIRDVVALVHSDAILQSTKISLRLNDNLPPVLGDKVQLQQVALNL
                     LLNAFDAMKGYSPLEREVKVSVAPYGDKMIKVAVSDRGTGLSVDKLDKIFEPFYTTKG
                     EGLGMGLSICRSIIEGHGGNLWAENNSDRGATFYFTVPMDRNGMMIGGETPAQVVLSA
                     EGPGLDKS"
     gene            complement(17088..17663)
                     /locus_tag="VF182_07955"
     CDS             complement(17088..17663)
                     /locus_tag="VF182_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437050.1"
                     /translation="MKKNILAVLSAMMLIGGISCGTGTQNKEGAAKPGAVTVDVVKLT
                     GIVKEIDREKKMVTVEGSGGRTVVIDATNARNLDQLKVGDRVNLTYAEEVALFVRKSD
                     EPPSLTESQSVELAPKGEKPGGLITKIIEVTGNVESIDTNNRTIALKGPAGNVRTYKV
                     SPEAKRFNEIKRGDQVVLRVTQAVALSVARP"
BASE COUNT         3864 a         5188 c         4627 g         4377 t
ORIGIN      
        1 gttggaaacg aactccaaaa attaaagcta ttccgacatt attctaaccc cgcgaggcca
       61 gcgagcccta cagtcgtagg caatgggcca gttccttgaa ggccgccccg cattccccaa
      121 aggggtgatg tttcgtacat cgaggcctta gagtcaagtg cgcgttcggg tgcccacggc
      181 ttccggtctc accgtcctcg ctcactgctg acggcgccaa ctattgtcgg attagggtta
      241 actgtaaaac cccgcgccgg gcgagacagc aaatccccat gaaaagacta tctgctttaa
      301 tttagctctg agtttacgaa cgcgaatttt ccgttctccg ccggtctagc gctttgtgcc
      361 gaaggttttt cgtcgccatg gagtttgcag acgttctgcg cttgctgcaa tcgcttgtcg
      421 gtaggatgaa aacggtcgcc aatatagttt tgctcttgga aattttccag aatgcgcacc
      481 caaatccggc aatcccagcc gagtttgttc caataaccca acccgatccc atccgcctcg
      541 agttctctgt ccgccgaata gttgtacagc cgcacgtacc atggcaagcc gagatcactg
      601 cccgaaccag cgaaattgaa gaaaccggag tttacttgtt ttattccctt gcccttggca
      661 tggcccgccg tctcgtgggc gatctcatgc gccacggttt ggcggagcag ccagagctga
      721 ccgggatatt ttaatgcgac cgatacgagc ctgtaactga tatagatctg cctgttgccc
      781 acacttatgc cgaaaagatc ctggcgcgga aagtccgaaa gaaagaactg gtattgcgaa
      841 aaattgtcgc ggtcctcgct gaccgctatg atacgcgctg cttcgcttcg gagtaagtgt
      901 tcgagccgat catcggatag ggtaggaatt tttgcttgtg tcgagcagga tatgaaaaag
      961 acgcaaagag ttatcgcgac acagaataga agtcgatcac gcgatcggtg gattggtttt
     1021 ggtgtcatac cggcagctat ggagtgttgg cgctaagcca gacgcgcgct ttttcgagat
     1081 cgccaggaag gaaaaattcg atttcggcct cgcgaaaacc tttggcgaga aaagcgtaaa
     1141 tagaatcccg ccatttatcc tctcccacga cggcgatctt tgcgatgtct ttatcgtgct
     1201 cggacataaa cgagatgtca ccccaatcag cgccgcgctt ccacccgctg aagttttcga
     1261 gaatcaacaa cgcacttacc ttgccgcagc gcaagatcgc ctggagcgct gcggtttgga
     1321 tctgagttac ctcggatttg tcgagctcgc cgctgatctt cgcactgatt atcggtcctg
     1381 ttccatcgat gatcgtaaat ggcatcgcgt ctcctcacat aagcggcaag tcgtccatga
     1441 taaagccata atcttttgac tgtctctggt ccctgatagt cacagatcga cgtgcctctc
     1501 ccagtgggtt ttcgtgttcg aaccggcaga ggtgcgtctt ttcaaggttt tggttttttg
     1561 agctctacgt cgaggcctga gaacgcgagg ttgaactttc gtcccgcgac tgtcgagacc
     1621 aggtttataa ccaccccttt ttcgttcttt atcgtggcgc tggagccggc gccgctctcg
     1681 gcctcctttg cttcagccat ttgatagttg ccggcaatgt cttccggact ttttaagtta
     1741 aatacttgac cggatagctc cgcagcggtg atttccgcat cgacgttccg gaaaagcccc
     1801 ctcgcctcaa acgttaaagg gtaatcctgg cctttgtagc ttaataagcc ttcgccccat
     1861 gagagcccgg tgcctggagc aaccgttctg gtagttatct tcaacgcgcc gtcggccgcc
     1921 tgagtagcag cgaaagaaag ctgcggcaac agcaagctgg ttccgacaat cagccatccc
     1981 agtaatacac gatgcattgt ctctcctcac ctgagcaacg acgcgcattt gcttgcgcat
     2041 cgagagttta tttgagctca cgacggccca gtctgctgcc gttgcgcgag gatgttatag
     2101 cgaatcgcga aaccttgaac attggacctt ggtccaatac cagggagagc ggtgcaaaca
     2161 gaccgtttgt gttcttagaa ggttctcgac gtcgggcggc tctggttttg agcaccggaa
     2221 acgcttcccg gcaaagaagg cgatcaaaca caggaggaag accggatcgg aagccattga
     2281 tcaaagttgt cattgccctt ttcctcctgt gagtttggcg gtttgcgcca gcgaaagcgc
     2341 ttgaaccgtt cgccatcgtt accgtgcttg ctgcgctttc aatcgatcca gctctctttg
     2401 ctggcgatcg atctcggcct gttgcgcctg gatctgtcgc tgaacgtccg cgtcctgttg
     2461 ctgccgcgcc tgcatctgat caccaatcag cgcgccaccg aggagaccga cggcggcacc
     2521 gataccggca ccagcccctg cattaccggt tgcgctgccg ataattgcgc ctaggccggc
     2581 cccgccgacg gtcccgacca ccgcgccttt ttctctggtg gtaagcggct cggagcaacc
     2641 gaaaaaaaca agcgccaaaa tgattgccga ggtattcgtc atctttttca ctactatctc
     2701 ctttggctct actacacaga aatattcgcc gagagattcc cggcagctac ttccccgtgc
     2761 cgactttgac tcgctgcggg tttaactgtt ggatcaccgt gagcatcgcc tcggcgaaat
     2821 gttgttttgt cttcgtctgg caactcttct ccaagcggtc ctttaaatca gccatattag
     2881 acttgccggc gacatcatac gtgtcgctta cgtagaaatt gtgcgcggtg agaaatccgg
     2941 caatccaatg ctccacaatc ctgtcgacga tctcgtatag ctcgtccttt gtatagcgcg
     3001 gatgctgctg ttccaaagta tcgagtctct tctctctaaa cttgacgtag tcctcgcagc
     3061 ttctctgacc tactccatag gcgaagtaac gattgttgct gtccattgca taaagattaa
     3121 aacctgccgc gatcatcacg agcgccaaag ccaataagaa tcctgccgat cttagaacat
     3181 tctgcttcat ttgaatttgc ctcccaccaa ttgtgaactg tgccgcaaag catctgccga
     3241 atgaatcgtg aattgatctt gtgcttcgag tctcgattct gaacgcaatc tttgtacggc
     3301 gggtctcacg gcttcgctgc cggcgttggc aagccttctc gaatacgttg cgagaacgct
     3361 ggatctttgt ctgcggcatg agcgcgttgg ttcacgtgcg agaccttttc cagatcggca
     3421 aaggatcgtg aagtgtcgag ccgaccaagc tcatagacgt aatccggcac atatccgctg
     3481 actagaattt tccaagacat cggcggcgac tctggattca tccgcgtgtg catgagaatg
     3541 ctggtcgtgc agttggtcgt gagcgtgttg taataactcg gtcggtcgcg caaatcgttg
     3601 atcgatttga tgtagtcgag aaaaactcgc cgcacgtttt ggatcggtcc gttaacgcga
     3661 tagacgtaca cgtcttcctg gggattgcgg taagtcgtgc gcacccgcac cacgtcgcgc
     3721 tcgtcggcga ccacgtaata gagttcatac tgcctgaaga accccgccaa ggtcgaatag
     3781 ctttcatttt tctctttacg cgtctcgatc gatacggcga ggtaatcttt gcccgcaaac
     3841 ccgaaactca ccatgatgtg agcgatggcc ttgcccgccc aatacaccgc gatcagatcc
     3901 acggagtcga gtttttgtaa atcataggtg cgggtttccc aacgcggcgt aaaatccgtt
     3961 tcggtccggt actcgaaatt tcgcagattg tggatcgtaa tcgtatcgcc gttgatcgtc
     4021 gcatagggcg tcatctccac ttcaggctgc caatcgcgat cgttcgatgc cgggatgcgc
     4081 agaaacaaaa cgacgagtat agccaaaacc gcgacaaagc cgagcagggt acgcccgcgg
     4141 cgcggcaaaa acaggaaagc tagaatggta ataaccgcaa acccagcagc gccgatggcg
     4201 cgcgcttttt ccgggagcag cggcgaataa tagagcatgc ccaccgccca gagagtcagc
     4261 gccccgacga cgagtcccac aacgaaccaa cctataatcc gcatacgaaa ctcatcttac
     4321 caggtaattg ccaccgtccc cggtgaaggg gatgaaaccc gctaaggtaa atagaagcta
     4381 tctggcgatt caagctcggg gtaccgccgt cctgtaggac cgctattctt tcttgcgctc
     4441 gaacagaatt cgtttgactt cttgaattgt ctccggatga ccctgtgtcg aatgactgga
     4501 gcggacgact ttttccgaga cgacgccctc gatgtgagcg ctggaatatt tcacgacgcc
     4561 gtcgtctccc tgctccggcg gaccatcacc ctcaaccgca atgatcgagt gcgccacgac
     4621 tccattcgca atcggaattc catgcaatgc cttgatgaag cgattgcccg gattcatgtt
     4681 ggtaatgctc gtgggcatac cgctgaatgg gccgaggacg aatccctgct tctgcaacgt
     4741 caccaagtcg aaactcagtt tggtaaaccg gccaggcaag ttaaccagcc aggaggcaaa
     4801 attgccgaga aggccaagcg cctgaaaact gccgccatgc ggtgtggcga caaagatgac
     4861 ttctttcacg aacggaagag gtttaaaaat aagggcgcgt gtcaacaaat cccgagtttc
     4921 cgcgctaaca tcgagttctt cgggagaaac actgaatggc caaagcgcca tgccgctgtc
     4981 gatcacactc attttcgtga gcaatccgcc ttggctatgt cccatgacta ccatgttctt
     5041 aagtccggcg tcggttcccg tgggatccaa ctctgacact aaatcggtca gcgcatcgcg
     5101 taaaagcagc gccgaatagg cgatcggatt tccggtgttg tacatgaaca gccagacttc
     5161 gtagttctcc caaaagcggc ggtcgttctc caattcatta acgagctccg cccatctagc
     5221 cgggctcgat gcagttccat gcaccaacac cagcggcgtg cgaccgggac ggtgtggatg
     5281 aagcatgaaa aggccctggc gcagatttcc ctcgcctata ttgaaatcgc ccgatcgaaa
     5341 gcctgcgatc tcgaaatccc aaatgggcgc gccttctaat gttagtgcaa gcgcagaggt
     5401 tgtttcatat tcaataggca cgtcgatgcc tgcaatgctc acggtccgcg cagaatacgg
     5461 agtgtaaaat tccagcttgc ctttcaactt tcccgatccc agcgcgccgc gcggatcatc
     5521 gagccgcaaa aatgccgtta ccggcactcg cagccgaggt ggaatacggg catgctcttt
     5581 acttgcggtc tcggccaacg gctcgataga cgcggcgagc gcagcgccaa tgccggggat
     5641 gcggtaacgg ttgcgcagac ctcgtattgc gatgtccgcc gcaggaacaa aacccgttaa
     5701 tcggtagccc gcccaaatca gatcatcctc gttgaagtcg atatcgatct cgccaaatgg
     5761 cagcgagaac ttcccagccc ttggcaccgg cttttgatcg atcatcgcag cgcgggtaag
     5821 tgcctggttg tagatatccg ccgcccatct aaagcgcgga tcgattgccc ggggagccgt
     5881 tccgtgttct ccgggaagta taaaggaata ggcgtaaacc gccgcggcca gatagtagga
     5941 tttatcctcg gtctgttcgg catacgcaaa ggaaagctcc accaatgcaa aaagacgatc
     6001 ctcatcgccc ttcggcgcta atccggcatg catctcggaa agcgcttttg ctggatcatc
     6061 ctggaaacgt tggtacagat tgagattcat cagctcgcgg gcagaaaacg agctgggccg
     6121 ttcggcggaa agaatgtttc ccgtaagaga ctggtaggcg atgcgcgggt taacgtagtt
     6181 gacaccgatg ggggtggcgc aacctgatgc gagaagctgc aatgtgacta tacagcagaa
     6241 caagccccgc aaactgattg ctaggagcgc tcgcttcttt tttgcctcaa cgagcatgcc
     6301 agctaattcc tatcgtccca tgcgtctgtc ataggcgcgc gaaatgcgac tggccaatcc
     6361 gggctcagcc ccttttggcc cgcgtgcgcc gatctcgatg cgcacactgc cgtccgcctg
     6421 gcttcggaga ttaagggtga cttcttggtc gttcctaacc ccccgaatga ctccgctgcc
     6481 tcggtcttcg gagataattc gcacgccttc gtcctgcgcc gccgacaccg ctgcactcca
     6541 agaacgatcg aacgcgctcg gcaccggcac cgggacagga gccggctcgg tcgcgcagcc
     6601 tgcgagagcc accaggctaa ggacgatgaa aatgatcagc tgcggctttt cagtatcatg
     6661 ctatccaaaa attctaattt aatagcgcgc acacgtaatc agctttggcc gatgagtacg
     6721 acgaatcatc atagccgttc tcgtgttacg cgcttggtcc ggtcggatcg ttgttaaatt
     6781 ttttagcgaa cagttgcggg gttgtatatt ggaccttagt ccaatcatgg agcgacaaag
     6841 gctgaaacct gagcgagctg aggctcgcgc gccgctagtt tatctaactg cttctacttt
     6901 caggtgggtt tcaactttcg cgcagcgcct gaaccgcgtc cgcctctttc ttcctcgctg
     6961 ctggactccg ttctgcgtcg ggatgcgaat agaacaaact aaagaaagcg ctcaatactt
     7021 cttactaaaa aatgatcgta cgctgcttac attctgctag gcttaatcgt agaacatctt
     7081 gtctcagcct tctcgggaca ctccagtcgt tcctcatttt caatccttga cttccgtcca
     7141 actcttgtcc ggattgtact ctttcatacc ttttgaaatg gaggcggtgt taggtcccag
     7201 attcttttga aagactttgc cctcgtgatt cacgataaag gtcatcacgc cggagttgcc
     7261 gtattcggct gggtaagcga ccaccgcgaa gccgccgatc attcttccct tgaccagata
     7321 actgtaggcg ccgccgggag cgtccttgcc ttgcgcggta agaatccgat aatagtagcc
     7381 gtgataggga acgggtgttg cggaaacctg ataaccttca ctcctcactc gcgccatgat
     7441 cggaccaaga ggactctgcg gctcacctcg ctgcgcttcc caatacaaac cgtttttctt
     7501 accgggatcg ctgacgaact tctgcgcata ttccaacagg gcattttggt ctcgatcctt
     7561 catcgcgtac tcgcgctccg catcgacgat tgcgagcagc gactgaatcg tcgacaactc
     7621 attttcaccg atccgccggt tgagaatctc ctccctgcct ttgtcggcat cgaacatcca
     7681 tgactggcct ttcttgacaa tcggaatcgg aaacggccag tcctgtttgc cgacgatcaa
     7741 gatcgtgttt tccccttcgg tggcgagccg gttcttttcg tcgtacgctg caagaaattc
     7801 ctcgcgtctc tgttcgtcgg caaccgaatc gcccgaaaac aacagctctt tcgcgcgcgt
     7861 gccaaagatc gcgagcagct ctttctgatt gttgctcttt gccgccgcga cagcggcttt
     7921 aaccgcctct tcggccgaag agaaagtctt ctgcgccaga gctgcctcag aactaatcgc
     7981 aagcgcgaaa atcaaggcga tcgcggcaaa gcagttccgc agacaagtcg agcgcgtttt
     8041 gttttgttta agtggcatag ccattgaatc ctccatcgat acactcacgg gctctaccca
     8101 cccgggccac tccaatcaaa tctgtcatct gaaaccgaac catggcccca atagtcccgc
     8161 ttagcggaat ctgcgatgcc ttaccgacgc cggccgccgc ctcctccgcc acctcgtcca
     8221 ccaccacctc ctctggcgcc gccaccgcca ccactgaaac ttcccctact cgcgctgccg
     8281 cgcgagctgg cgctggctgc ggcgctgcca ccgccgctaa cgccttggaa agcaccgcct
     8341 cggcccgagc caccgccgcc gccacgatcc attgttcctg cgcgattacc gccggcacca
     8401 cctcccaggc cggcggcacg gtcaccgcca ccgccttgcc cgccaagccc gccgcggtca
     8461 ccagcaccgc tcaaaccgcc ccgatcgcct gctccagtgc gcgctagatc ttgccgtccc
     8521 tggtcggctc gtccgcgaaa ctgctcccgt gactgaatcg cgtcgttact gctcgcccgg
     8581 ttaaactttt gcgcagttcc ctggtctcga tacgacaccc ctttgcggtg ctccgggtca
     8641 tgctgccact ttccctgacc cctttgatta ccttgtcctc gttcctgcaa tttttgtttg
     8701 gcttggtctc ggttgatatt tgtattgatg ttggtgtttc ggtcgacgtc gatgtcgacc
     8761 tctccaccat tccaattgga attgccccag gcgtatcccc aggccgcgcc catggccatg
     8821 cccgcgccga accacatcgc ggatgatgcg acatagcccg gcggataata ggtgtagggt
     8881 ggataagctg gatacggcca cccgccataa acgaccgtag gattgtaagt tggtacgtaa
     8941 acgacttgcg gattagccgg ttcgatcttt atgatctttt cttcgacgat gacggtttgt
     9001 tctttggtgg tctgaagatt gccggaggcc tgcgccttgg cgcgcaggct ctggatcgtg
     9061 tccaaaacct gtttctgatc cgccaagaat gcatcgccga gtttttgcgt ccattcgagc
     9121 tgttcgctca tcatggtaac tacctggggg aagttgacca gagattttac gctggggtcc
     9181 cactgctgtt tctcgagcgc ggctgtgagc gcctcgcctt tgaggctggc gttttgtttg
     9241 atccagcggt cagcctgcac gacttccaga ggataagtcg ctgccatcaa gatttgcgtt
     9301 accagcgaat ccggatggag cgcgatcggc gctaatatct gctccagttc ttccggtttg
     9361 aagaccgcgg cggtatcgga ctgttgggcg atcactcccg gagttatcgc cagcagcaga
     9421 accatcaacc acacgaatcc tcgcgcaaac aatcgcttcg ccaacatggt cactccttct
     9481 ggtcaagtac cgagtgtcgc ctttcgagta gaatcgcact ttatctgtgg ccactcatca
     9541 tccaatcatc gcttaaccta atccgacaac gttgagtacc gtcagctgtg agcgaggacg
     9601 gcgagaccgg aagccgtggg cagccgagcg cacagttgct ttaaggcccc gacgtaccaa
     9661 acatcaccct taggggaatg cggggtggcc ttcaacgcag tgtcccattg cccgcggctg
     9721 taggggtcgt tggcttcgcg tggtcgaaat atatcggatt ggagttacca ttcgccccgg
     9781 gttcctcggg ccttttcatc acttgaactt gaggctcact ccggatgcgc ccaacgtgag
     9841 ttgagttccc gactgctcgg attgtaaagc gatgaagact ccgttgccgt tggccatcac
     9901 gagctcgccg ccgccgccgg ccactgcgaa tgtcgcttga gtggcgaagt aattgccgga
     9961 aaaatccgaa ggctgcttta gaaagtaaac tctgccggtg gcgctaacgt ccgacacacc
    10021 gagatccagg agactcaaac ccgaaacagt tatcgggtag tccttgcctt gatagctaag
    10081 cacgccctcg ccccagttca ctcccgcacc aagggcaatc gatcggctct tgagtttcac
    10141 tgtagcgtcc ggcgtctggc cttccttacg gtacgagggg gtggccttat cctgcgccac
    10201 tagcagcgct tccgcgtttt cgtaggttgt ccagccgatg ctgcgcagct cgttacgcgc
    10261 cctcgggctg agatttccgg tcagccaaag ctccttccgg gtaacgcccg gcaactggtt
    10321 cgccaacatg tcggtattgg ccactaaccg agctagagaa tcggtccaaa ccagatgatc
    10381 tatcggaaag ttgaagacca aggctccatt agccgcgcgt acgccgggca cctggccgac
    10441 agagacaaac tgtgatagcg gcgagactgc cttatggtag ccggcgtaca tctctgcctg
    10501 acgctgacga aatagagcca tatcggcatt gttcgtgagc gccgccagtt gcacgaatct
    10561 ttcggcgtcg cctatgccct tcatctcgtc caatgcgaag actaggacgg tctgttcgcg
    10621 cggcgaaaag atcgattcat tcatgaacat ttcaacggta gccggatcga ttcccatggc
    10681 gttgagcttt tccatattga tccgccgcaa atcagccggc ggcgtgttct tgaatatctc
    10741 tctggtgagt ctcgctgtgc cgaccgctgt catcactgtg ccagcagtgc ccggcaccgc
    10801 ggccatcgcc gcagagaccg taagtccgcc ggcaaatccg acccaggaaa tttcattcaa
    10861 gcgatcctgg aggacttcgt tcctggaata gacatcaacg cctaaatcat aggcgtactc
    10921 gcgtttgtaa tttgaaaagc cgatgaggct tttgaatcgg ttatcttcaa cgtcactgcg
    10981 tttggcgccg aaaatgctgt ccccggcgcg tcgaaaggcc acagccacac cacttgcggc
    11041 agtcttaacc gtttgtaccg gttgaaacac catgtcctta gcggcaacca tggcggacaa
    11101 agtcgattcc ttgactgccc tcccgtactc tgtcgtgcct tttagtttgt ccatggccgc
    11161 catggcgtta atttcctgga tgcgcgctcg aagcaaagca gttgaaacgg ccctgaactg
    11221 gccaaatctg gagtcgatcg tgtaggtatt caaaaagccg tcgtttacta ctcgttcttg
    11281 aacctgatga ttgggccctt tcaccagttc gggcggcaga atttttgacg cactgagaac
    11341 cggcagcgac tcatatccgg tttgcgctgc cgcggagccg cgcttcggtt gaagaactaa
    11401 gataaccgag agaatgatca tcgcgccaaa agctatgttt cggcgaaacg gagagaattg
    11461 ctgcgactgc atcatctaaa atacctccag aatggtagcc aatgctcaat cttgtatcga
    11521 tttctttggg gtgacgcgtg ccggaatctg tgttgaactc aagtgtattt catggcttgg
    11581 tccatcgttt taattagcag ttcatcgttg aatggtttac gtaaaaaggc cactgctccc
    11641 gcggccagcg ccttctcacg caccccaacc tcatcgtgcg cggtgataaa gatcaccgga
    11701 attgttttgc cgcctttggt gagcttttcc tggacgtcaa gtccgttgat cccctgcatt
    11761 tgcacatcaa gaatcaaaca gtccggttta aacgacggca tcgcgtgaag aagatcgaga
    11821 aagtcttggc ccgagctgaa cgtctgcgcg ttcatcgcca acgagcgcac caaacgccta
    11881 acggcgcggc atacagattc atcgtcatcg acgatggcga tgagcggttt tgattttttc
    11941 aagccaatag gtctctctgc gcttgtggag acattaatag aaccagtcat gtcgttgctg
    12001 ggaggggact ctagcgaacg agaaaaactc cgtatattgg actttggtcc aatatacgag
    12061 ggcgagcgtg gaggagcgaa ggcacagaag cgaacagttt caatgctatc tatggtgctt
    12121 ggcctcgatc actctgcagt tatccatctc tactgcggaa tccgggcgaa tgaactctat
    12181 tgtctttgat aactttcggt tgctggccgg gatcacactt ctgcatagat cgaggtaggc
    12241 tcggattatc agtccaaccg ccctgtagcg cggctttgaa tctgtcgtct cgcttgtttg
    12301 gagcaggcta cgattcaagc catgcgtgga tattggacct gggtccaata cgggagagga
    12361 aggatgggag attggcagga tggagtgttg ggatactcga gaggctaata atggaagtgg
    12421 agcattactt gccccgttcc attactctac tgtttcagtt tttatttttt tgggccggga
    12481 tgccgacttt ttccgccagg cgtaccagtt cggcaagcga ttccactttc attttttcca
    12541 tcacgcgcgc gcggtggacc ttgacggtct tttccactgt tcccagctca aaggcaattt
    12601 gcttattcaa caaaccactg acgactaaag tcatcacttc tccctcgcgc ggggttaagc
    12661 tggttaatcg cgtttgtatt tcttccagct ccaggcgctc ggtataatcg tgactagccc
    12721 gggcaagcgc ctgttcaatc gccctcaaaa ggtccttgtc ctttactggc ttgggcagaa
    12781 aatctacggc gccttgcttc atcgctttca cactcattgg aatgtcgccg tgacccgtga
    12841 tgaagataat gggaagcagt gtgttcgcag cctgtaatgt ctgctggagg tccagcccgc
    12901 tcagacccgg catgcgaaca tcgaggacga ggcaggcagg gttttggtcg ttggcctgcc
    12961 agtactcaag aaaatcccgc gccgaggcga acgcttgcac cgcatatcct cccaccttga
    13021 tcaggcgcgt gagcgcccga cgcacggagg catcgtcatc gacgagaaac acgttggagg
    13081 gtgtcgacga ctcagggtag acggtcgaag atgtacgcgg gggagtgtgg aggtacaagt
    13141 gtcgagcggg attcggcatt tgtggtcttt tactttccca tcatcgagtg attgagttga
    13201 agcgtttgtt ggtcatgtcg cgagttagcc aaatcgcctt tcgtgtcggc ctattctaat
    13261 cctgctcggc ttgcttaaac tcttaatatt gcacggatcg attcgcactg cccactgaca
    13321 cagcagaaca tacttatgtc tgccgaaact caatcacaga atcgtagtga taacagctgg
    13381 actatgactt atctagtcca ggcccttccg cactcagtac aacctgtgcg ggcgtctccc
    13441 cgccaatcat cattccgttc cgatccatcg gcactgtaaa ataaaatgtc gcgccgcggt
    13501 ccgaattgtt ctccgcccag agatttcccc cgtgcccctc aataatcgag cggcagatcg
    13561 aaagccccat gccgagccct tcgcctttcg tggtgtaaaa cggttcaaag attttatcga
    13621 gtttgtccac gcttaggccg gttccgcgat cactcacggc taccttgatc atcttgtcgc
    13681 cgtagggcgc cacgctcact ttgacctctc gctcaagcgg cgagtagccc ttcatcgcgt
    13741 cgaaggcgtt taataaaaga ttcagcgcaa cctgctgcag ttggacttta tccccaagca
    13801 ccggtggcag attgtcattt aatcgaagcg agattttagt gctttgcaga atggcgtcgc
    13861 tatgcaccaa ggccacaaca tcgcgaataa gccctgtgaa atcgatcgat gcaaactcga
    13921 tcccttcttt ttttaccaat gcccgcatgc ggcggatgac ctcacccgcg cgcctgttgt
    13981 cttcaacgat gtcccgcaat atttcgtgca cctcttcgat atcggccggt ttgctgctca
    14041 agaaacgcag cgccgcttgg gcgttactga ggatcgccgt aagcggctga ttgagctcgt
    14101 gagccagaga ggccgccaat tctcccatgg tcgaaatccg cgatacatgg gccagttcca
    14161 cgcgctgttc ccgcagttcc atttccgtct ttttacgctg ggtaatgtcg gttgagacgc
    14221 ccaagacctg ctttgccgag ccatcacttt caatgatcgg gcgcttcacg gtctgaagcc
    14281 agcgcacccg ccccccaccg ttggttatgc gctcttccgg tataaaacgt tcctgcaaag
    14341 tgtcgatgac gtccaaatcc gcttgccgga aggactcgac ctccgattga ttcgggttaa
    14401 aatcggcatc ggtcctgccg atgagattat ccacggtcgt accgtatgcc tcagctaccg
    14461 ccttgtttgc caacgtgaag cgcccctcac ggtccttggc aaaaataaag ttcggatcga
    14521 tgtcgatcac ctggcgcaag aactggcgct cacttctcac ggcgtcctcg gcttgtttac
    14581 gcgcgctgat atcgagcata aggccgcgca gctgcgtttt tcccgtgccg ttctgatgct
    14641 ggcaattgac gatgtcgtga atccagacaa cttcaccaga cgctttgatc attcggtact
    14701 cgaaattgaa gtcctttgcg ctctggctca tcgtcaaaca cgtgttgacc gcctgctccc
    14761 ggtcctcggg gtggatatga gatacccaaa agtctttttc gtaccactgc tccaccggat
    14821 aacctaggag cttggtagcc tgcgggccga cataggtaaa cacccaattg tcgctatcgg
    14881 cttgccatat gacggcagcc gtggtttcca ccagccggcg aaaactttcc tcgctttcgc
    14941 gcagtttctg ctcggctcgc ttcctctcgc tgatatcgat gaccattccc atccgtcgcc
    15001 tgtcgcggcc acttccggac tcggcggtaa tccgaccctt tgccaacagc caccggttgg
    15061 tgccgtcggg gagaacgatg cggaattcgc cctggaatgg cagcccggcg gtttgcgctt
    15121 ccttgagttc cgaaagcata cgcttcaggt catcagcatg tatgcgctct tgtatggctt
    15181 cgaacgtaac tgtttcgttt gaaccgaaac caagaagaga acgcccgggc ccgttgatcc
    15241 agacctcggt agctgtcaga tgacgcgacc acagccccaa ctcgcctgca ctggcagcat
    15301 gttccatcag ctcccggctg tctcgcaact ccgcctcgac cttgcgccgt cggcgccgct
    15361 gtagaagcaa atcggcgatc attgtggtct gaagccctat aatgatcagc gcggcgatga
    15421 tgtaccaccg gtattcctgc cagaacgact gcggacgaaa tcgaactata ctgttggcag
    15481 gcaggcggtt ttcgggaatg cgccaacgtt gcagggcccg ccaatcgaat gtgggtacgg
    15541 tgtcagtacg aatttccaag ggcagcgagg ccggcgctgt gccggttaaa acaagacgag
    15601 ccagctctcc cgccctttgt ccgaaagcct ctatgctcgc gagcgctcca ccgacggctc
    15661 ccgtgcctaa accagtctcg gtcatgacat acacaggcgc attcgcctcc tgcgccagtg
    15721 ttcgagccac ctcggcggaa atcaaagcct gcccggcccc atccctaaac acgcgactga
    15781 acaaaatcgc cgtgtttgcc ggtaggactt tcacggtctt attcaattca tccatcctga
    15841 aattgtccca gaactcgatt ttcagccggc tcttaaacgg ctctgcggcg tctcttattc
    15901 tctgcgtgac ttgctgatca gtctccgtag tcccaccgat gacgatcacg cggttcactt
    15961 cgggttgaag acgcccgatc aggtctagcg tggcttgagg attgacacgc tgcgccacgc
    16021 cgcttacgag cttgccaaac tgcttccgat taagttcttc ttccgtgaag ccggctacaa
    16081 tgattggtgt gttgggaaac agttgcggca gtagcgttcc cgtaattccc aggttgccgg
    16141 tgtaaaccag gattacgaga tcgggacggt tctcactgta tttagcgctt aaatagtctc
    16201 ggaagacttg ctgataacgt tcagtcggaa accgtaccag atcgagattc tcggcatcga
    16261 tttcgattcg cgccgtttgg atttttcgaa gcgccgcgag caactgctga tcggcaatca
    16321 caaatcccgg cgcaagccgg cttccgactg agatgatcaa gacacgcttc actggcgccg
    16381 gttccgcatt gctggcgctt cgtgccggcg tgaaacaaaa gagcacagcc aacaacagta
    16441 tcatcaacgt cttggaacgc agcgcgccca acgattggta agagctcgaa ccagacatac
    16501 aatcacgccg accgaaacgg gaatggatca aagccaaccc gttgtgaatc gcaacgttgc
    16561 acgttgttcg ccggcgcagc agaagcattc tcgcaatatc gtaaattgat gcatcacatc
    16621 ttctcgtgcg aatgataggg cctgtactac ggaaatcgga ggtttgcaaa tgaccaattt
    16681 tcgacgtttt ctatgggttt gcgtctcgtc cagacagcac atgccgtcca tcaccgtgac
    16741 gtcgagctgc acacatgcag caaagcgcat cgaatgagtt cgagcggacc gtttgcagca
    16801 cgttcagcca caaattcgca gcgagcagaa tgtcccaaga tacgtgacgc tcctgatgga
    16861 agcggacgca ggaagcggtg cccacgagct attcgcgtcc gtaaaacggc cgtagactcg
    16921 ctagccgtga agctgtttgc cctgatggtt gtcggtggga tggttacgtt ttcgacgtgc
    16981 caagtcgatg gtagataaat tgcttcggcg gagttcatcg aaaccgtgcc acgcgttctc
    17041 agcaacttgc ttggcgatta ttctactttt cttgttctag ctctcattca gggtctcgcc
    17101 accgacagcg ccacggcttg ggtaacccgc agcacgactt gatcgcctct ctttatctcg
    17161 ttaaatctct ttgcctctgg actaacctta tatgtccgga catttccggc cgggcccttc
    17221 aatgcgatgg tccgattatt cgtgtcgatg gattcgacgt tgccggtcac ttcgattatc
    17281 ttggtgatta aacctcccgg cttttcaccc ttcggggcca gttccacgct ttgcgactcg
    17341 gtcaggctcg ggggctcgtc tgactttcgc acgaaaagcg ctacttcttc ggcgtacgtc
    17401 agattgactc tatcaccgac cttcagttga tcgagatttc tcgcgttcgt ggcatcgatc
    17461 acaacggttc tgccgcccga cccctccact gtcaccatct ttttttctcg atcgatttct
    17521 ttcacgatgc cggtcaattt aacaacatcg accgtcacgg cgcctggttt cgcagcgccc
    17581 tctttgtttt gagtgcctgt cccgcaactg atcccgccga tcagcatcat tgccgacaac
    17641 acggctaaaa tattcttttt catctctctc ctctcctctt aaaatggtta aatcgcgcga
    17701 actcaaatac gcctgaagct cgcctattga cgtcgcccgc gattttccct gggctttggt
    17761 tgttgtagtg cgaggccacg gccaatacac cgatgatgat tatttgtggg ccggagactt
    17821 tcaccgtttt ttgttctctt tggcttcttc gagctcgcgc ttgttatcga cgttagggcg
    17881 ggccgtcaac tgcatttgaa tcgtaatgtt gtaccagaga atgagcaaga tggatactga
    17941 ctttagccta atgccggcat caccgcgttg cgaatctgtt caccgatgat tctccgaaaa
    18001 tgcttatgca agcccaattt cgattacgtc actgcgcatt cgcctcaatt aaaatc
//
LOCUS       DASUVS010000139         5749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1362448, whole genome shotgun sequence.
ACCESSION   DASUVS010000139 DASUVS010000000
VERSION     DASUVS010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362448"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="VF182_07960"
     CDS             complement(<1..1515)
                     /locus_tag="VF182_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEX6437051.1"
                     /translation="MPTKTIEIAGRPIILDARPDRLDLRDLPYRPSVVCLAPIYPGEK
                     DISKLLPRYLAAGLVLDQESEGACTGFGLAAVINYLLWRRSGLKLKSKDKVSPRMLYH
                     LARFYDEWPGEGYEGSSCRGALKGWHRHGVCTDTLWPYRDENGAHFIPPTDGWETDAI
                     GRPLGVYYRVDRQSVVDIQAAIYDTGAVYVSANVHEGWDVQSKKGAATHRTLPVINAS
                     NKGLGGHAFAFVGYNDIGFIVQNSWGARWAASGFAVLPYDDWIANGTDAWVMSLGAPV
                     QNLRQRQYFVRTSAGLSSTPTSSGLIGPALARRKPSEMCWSEQEAYWHTIITGNDGLV
                     INRLPHVADENDNVGWVSYQQPLKWFKANARTTPWRIAVYAHGGLNSEGDSIERIRLL
                     GPCFRENSIYPVFTTWKSGWQETFGNMLSDGMKKLFGDQIPPRRGLGDMLVEASDRAL
                     EVFLRGVIGKSIWSEMKENVIRSTETGRGIDQLAEQLKRLAADAGGKLQVHLIGH"
     gene            complement(1702..2517)
                     /locus_tag="VF182_07965"
     CDS             complement(1702..2517)
                     /locus_tag="VF182_07965"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX6437052.1"
                     /translation="MAKYALCIGINDYPGTGSDLSGCVNDVRDWSALLQKKEFAVLQI
                     IDKKATGKNIRSAIESTIGGAKSGDAVIIQYSGHGSYVPDENGDEPDGTDECICPYDI
                     NSKGPITDDELFELYSSKKTGVKLLVISDSCHSGTVAKFAPITTPPTIKGRGAPIRKV
                     RFLPPAAFLSKRDIGKLGIGRGLRRSTPPGRYAALLMAGCQDTEYSYDASFQGRPNGA
                     FTFVAIRALSGLSPKASYKQWYQAIRKALPSQQYPQSPNLYGTSRMKAWKALA"
     gene            2794..3849
                     /locus_tag="VF182_07970"
     CDS             2794..3849
                     /locus_tag="VF182_07970"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6437053.1"
                     /translation="MNSHKSSVLSFLTGALLAGLLSAAPALAQSDFYKGKTITVYIGT
                     TAGALYDQWGRILATHMVKHIPGNPNVIVQNMPGAGHMIAANYVYKTTKPDGLSLIGS
                     IVPTLYFDQLVGRKEAQYDWAKFVWIGSPVQGELQMYMRADTPYKTMDDVRNAKEPPR
                     CGAQGTSDTAYYVPKLFEEVIGTKFNLVQGYPGGPEIDLAVERGEIHCRAFTIEAFFS
                     REPYHTWRKKGFVRNIIQTGKKRDSKLPDTETIWELMDKYKTPEAGRRLATVMLASGA
                     LGRPMLATPGVPPDRVKILRDAFNKTMEDKEFLAELEKRQFELEPSSGEELEKIVKDV
                     MNQPPEIVARIKKLLGQ"
     gene            complement(4177..5175)
                     /locus_tag="VF182_07975"
     CDS             complement(4177..5175)
                     /locus_tag="VF182_07975"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437054.1"
                     /translation="MTSTLCKISTLLLALAVFLLSSFASRPIFGADRIRIAYTSPGPQ
                     HGVLWIGDVAGVFKKNNLDLEIIYMPGNISMGSLIAGELNFGQMTGALMSPARLQGSD
                     VVMLVSIQELLDDRLVVRPNIKTPEDLKGKRIAVSRFGAASHMRVLNMLPRFGLTDKD
                     VTFLQIGDTPARIIAMVGNSADASSFSPPDHLAAVQSGMRVLLNMAELNIYYQGTGLV
                     ASQRYIAKNRDIAKRMVKSYVDTIHIVRTNPEVAKRAFVKYRKTKDEKQLEDAYQTLL
                     EIVKPKPYPNLESFKTIFKDVSDRLPAAKTANAKEFVDTSFLEELDKSGYIDGLYR"
     gene            complement(5311..>5749)
                     /locus_tag="VF182_07980"
     CDS             complement(5311..>5749)
                     /locus_tag="VF182_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437055.1"
                     /translation="SLNPPTVFFAQRKGFHRVLDIGSLVEMPGGGLTTLIKTITSKPD
                     EVRKVIRALQTAKDAIRKSKDKTLELMVRALKMDRDIASSTYDVYLTSLSIDGVPARE
                     GMNNLARSVQSQARFADRKVSFEDVADVSLAKEVAKELGYKVK"
BASE COUNT         1284 a         1519 c         1592 g         1354 t
ORIGIN      
        1 atggccgatc agatgaacct gcaacttgcc gccagcatcg gcggccaatc gtttcaactg
       61 ttccgccagt tgatcgatac cacgaccggt ttcggtggaa cggatcacgt tctctttcat
      121 ctcagaccag atgcttttac cgataactcc gcgaagaaat acttccaaag cgcgatcgga
      181 ggcttccacg agcatgtctc ccaatccgcg ccgcggcggg atctggtcgc caaataactt
      241 tttcatgcca tcgctaagca tgtttccgaa ggtctcctgc cagccgcttt tccatgtggt
      301 gaacacggga tagatgctgt tttcgcggaa gcagggtcca agcaatcgga tgcgctcgat
      361 cgaatcccct tcagagttga gcccgccgtg ggcgtaaaca gcgatgcgcc acggcgtggt
      421 gcgcgcgttg gctttgaacc atttcaaagg ctgctgatag cttacccatc cgacattgtc
      481 gttttcatcg gcgacgtggg gcagacgatt gatcactagg ccgtcatttc ccgtgatgat
      541 ggtgtgccaa taggcctcct gttcggacca gcacatctcc gacggtttgc gccgggcgag
      601 agccgggccg atcaggccgg aactagtagg ggttgaggaa agcccggcgc tcgttctgac
      661 gaaatattgt cgctgtcgca ggttctgaac cggcgcgccc aacgacataa cccaggcatc
      721 ggtgccgttg gcgatccaat catcgtatgg cagaactgcg aagccggaag cggcccagcg
      781 cgcgccccaa gagttctgca cgataaaacc gatgtcgttg taaccaacga aagcgaaagc
      841 gtggccgcct aatcctttgt tgctggcatt gatgaccggc agggttctgt gagtggccgc
      901 gccttttttc gattggacgt cccaaccttc atgcacgttg gcggacacgt agactgcgcc
      961 ggtgtcgtag atcgccgctt gaatatcgac caccgattga cggtctactc gatagtagac
     1021 accgagcggg cggccaatcg cgtcggtttc ccatccatcg gtgggcggga tgaagtgagc
     1081 gccgttctca tctctgtagg gccagagagt gtccgtgcat acgccgtggc gatgccagcc
     1141 tttgagcgct ccgcggcagc tcgatccttc gtaaccttcg cccggccatt catcataaaa
     1201 acgcgccagg tgataaagca ttcgtgggct aaccttgtct ttactcttaa gcttcagccc
     1261 ggatctacgc cacaacaaat agttgatcac tgccgccaaa ccaaatccgg tgcaggcgcc
     1321 ctctgattct tggtcgagaa cgagaccggc ggcaaggtag cgcggtaaca gcttcgaaat
     1381 atccttttcg cccgggtaaa ttggggccag gcaaaccacc gatggacgat agggtaagtc
     1441 gcgcaggtca agacgatccg ggcgcgcatc caatataatt ggccggccgg caatctcgat
     1501 agttttcgtc ggcatctcga agtccttccg tcgatcgcaa atgactaaca gtttattgaa
     1561 gtctgattac aggctgctca aaactctaag gcgacacgcg cgaatgacaa ggatgaaagc
     1621 ggaaatcgga aagcggaatt ttagttttca gctttcatat ttcatctttt ccaaagccga
     1681 tgaggctttt cagcagtcct gttacgccaa cgccttccat gccttcatac gcgaagtgcc
     1741 ataaagattg ggggactgcg gatattgctg tgaaggaagc gctttgcgaa tcgcctgata
     1801 ccattgcttg tagctcgcct tgggcgagag accggacagt gcgcggatcg cgacaaacgt
     1861 gaaggcgccg ttggggcggc cctgaaacga tgcgtcatag ctgtattcgg tatcctggca
     1921 gccggccatt agcagcgcgg cgtaccgtcc tggaggagtg gaacggcgca aaccacggcc
     1981 gatgccgagt ttgccgatgt ctcgttttga taagaaagct gccggcggca agaagcggac
     2041 ttttcggatt ggtgcgcccc tgcctttgat cgtcggcgga gtggtgatcg gcgcgaactt
     2101 cgccacggtc cccgagtgac atgaatctga aatgaccagg agcttaacac cggtcttctt
     2161 gctgctgtag agttcgaaga gctcatcatc ggtgatcggc ccttttgaat tgatgtcata
     2221 ggggcagatg cattcatcgg tgccatccgg ctcgtcgccg ttttcatccg gcacatacga
     2281 tccatgcccg gagtattgga tgatgacggc atcgccgctt tttgccccgc cgatggtcga
     2341 ctcgatggcg cttcggatat ttttgccggt tgccttcttg tcgatgatct gcaaaacggc
     2401 aaattccttc ttctgaagca gggcgctcca gtctcgaaca tcgttgacac agccggaaag
     2461 atcgctgccg gttcctggat aatcattaat gccaatgcac aatgcgtatt tcgccatgaa
     2521 ttcctccgtt cgatttttgg acgtatgtta atatccaaac aaaattccgt ctacgaaaat
     2581 atttgattag gtagggcgac gaagagaaaa ttactgcacc ggaaaagtga cggtcaattt
     2641 tacgcgctgt tggcctcgag attcggtggg agtggatctg ctttcatacg tttgacgatg
     2701 ctgagacgga gagatgccga tccttgacag gtgtgccgtc catgttaact tacagcgcat
     2761 ctatttatta gttccgcttc aggagggcct ggaatgaatt cacacaagag ttccgttttg
     2821 tctttcttga ccggcgcctt gctcgccggt ttgttatcgg ctgctccagc gttggcgcag
     2881 agcgatttct acaaaggcaa aaccatcacc gtctatatcg gcacgaccgc gggagcgctc
     2941 tacgatcagt ggggacgtat tctcgcaacc catatggtca agcacattcc cggcaacccg
     3001 aatgtgatcg tgcaaaatat gccgggcgcc ggccatatga tcgcggcgaa ttacgtttat
     3061 aagacaacca agccggatgg cttgagcttg atcggttcga tcgtgccgac gttatatttc
     3121 gaccaactgg ttgggcgcaa ggaagcgcaa tacgattggg ccaaatttgt ctggatcggt
     3181 tcgccggttc agggagagtt gcagatgtat atgcgcgccg acaccccgta caaaacaatg
     3241 gatgacgtgc gcaatgccaa ggaaccgcca cgctgcggag cgcagggaac atcggatacg
     3301 gcctactatg tgccgaagct gtttgaagaa gtgatcggca cgaagttcaa cctggtgcag
     3361 gggtatcccg gcgggccgga gattgatttg gcggtggaga gaggcgagat ccattgccgc
     3421 gcttttacca ttgaggcgtt tttttcgcgt gagccttatc atacctggcg caagaagggg
     3481 tttgttcgaa acatcatcca aaccggcaag aagcgcgaca gcaagctgcc ggataccgaa
     3541 accatctggg agctgatgga taaatataaa actccggaag ccggccggcg tctcgccact
     3601 gtgatgttgg catcgggcgc cttgggccgt ccgatgctgg ctacgccggg cgttccgccg
     3661 gaccgggtga agattttgcg cgacgctttc aacaaaacca tggaggataa ggaatttctc
     3721 gccgaattgg agaaacggca gttcgagctc gagccatcgt cgggcgaaga gctggaaaaa
     3781 atcgtcaaag acgtgatgaa ccagccgcct gaaatcgtcg cgcgaatcaa aaagttgctg
     3841 ggccagtaga cagaccatac agttcgaaaa agccgccgtg cgtaaaaatg cacggcggct
     3901 tttttatggt ggccgatagc ccggattatt catgctcaga agaaaaacta cgacactgtg
     3961 tcgatcgaac ggtgagcctg gaggagtgaa gacatttgga cgaacagctt caatgaagtc
     4021 tttcgtgctt gattccctgc tcgcactttc ctaggcgtcc gctccacaaa ctgtattatg
     4081 caggcggctc caaggcacag gtccatttgt cttcgttccg gagggctcgc ccttcggtcg
     4141 ccgtcaccaa ggaatagtcc gagctagtct aatacatcag cggtagagcc cgtcgatgta
     4201 gccggattta tccagctctt cgagaaaact cgtgtcgacg aattctttgg catttgccgt
     4261 cttggcggcc ggcagccggt cgctgacatc tttgaaaatg gtcttgaacg actccaggtt
     4321 gggataaggt ttgggtttaa cgatttctag caaggtctga taggcatctt ccagctgctt
     4381 ctcgtctttg gtcttgcggt atttgacgaa tgctcgcttc gccacttctg ggttggtcct
     4441 cacgatgtga atcgtgtcga catagctttt caccatgcgt ttggcgatat cgcggttctt
     4501 ggcgatgtaa cgctgcgacg ccaccaagcc ggtgccttga taataaatat tcaactccgc
     4561 catattgagc aggacgcgca tgccggattg tactgcggcg agatgatcgg gcggcgagaa
     4621 gctcgaggcg tccgctgagt ttccgaccat cgcgatgatc cgcgccggtg tgtcgccgat
     4681 ctgcaaaaac gtaacgtctt tatccgtgag gccgaagcgc ggcaacatat tgagcacgcg
     4741 catgtgcgaa gccgccccga aacgggacac cgcaattcgt ttgcccttga gatcttccgg
     4801 cgttttgatg ttgggccgga ccaccaatcg atcgtccagc aattcctgaa tgctgaccag
     4861 cattacgaca tcgcttcctt gcagccgggc cggtgacata agcgcgccgg tcatctgccc
     4921 gaagttgagc tcgccggcta tcaacgatcc catcgagatg tttcccggca tatagatgat
     4981 ctcgaggtcg agattatttt tcttgaaaac tccagcgaca tctccaatcc agagaacacc
     5041 gtgctgcgga ccgggggaag tataggcgat gcgtattctg tcggcgccga agattggtcg
     5101 gctggcgaaa gatgacaaaa gaaacaccgc cagcgcaagt aagagtgtgc tgattttaca
     5161 caatgtagaa gtcatatcgc cctccggatc tcgcggcatc gatagcttaa aaagtctgtc
     5221 aaaacagtag ccgcaaaatt atcgcgaggg tgtggcaccg tcaaggaatt attctggttg
     5281 gcggcggttg attcggtagt cgttgcagtt ttacttcact ttgtacccca gctccttcgc
     5341 cacttccttg gccagactaa cgtcggcgac atcttcgaaa gaaactttcc tgtcagcgaa
     5401 ccgtgcctgc gattgaaccg atcgagcaag gttgttcatg ccttcgcgcg caggcacccc
     5461 atcgatgctc aggctggtga gataaacatc gtaagtcgag gaagcaatgt cgcgatccat
     5521 cttcagtgcc cgaaccatga gctcgagggt tttatctttt gattttcgga ttgcgtcctt
     5581 agccgtttgc agcgcgcgaa tcactttcct gacttcatcc ggtttgctcg taatcgtttt
     5641 gatcaaggtc gttaaaccgc cgccgggcat ctctactaac gagccgatat ccagcacccg
     5701 gtgaaaccct tttcgctgcg caaaaaatac tgttggtgga ttgagcgag
//
LOCUS       DASUVS010000140         3258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1362859, whole genome shotgun sequence.
ACCESSION   DASUVS010000140 DASUVS010000000
VERSION     DASUVS010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362859"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..816
                     /locus_tag="VF182_07985"
     CDS             154..816
                     /locus_tag="VF182_07985"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437056.1"
                     /translation="MEFIRLLPEGVGCIPMYAGIKEHSERGYLSAMETYHAKMAELAE
                     IGVDLMHPEGGPPFMVRGHKAEQEIVGGWEAQYKVPIFTSGMSQAQALRALGIKKFVG
                     CTYYRDQKMNAIFNRYFTDAGFEVLGMEGMDTSPDEADKISTETIYQHLKASFNKFPK
                     AQGIYLLGSGAWGVKDIIAAENDLGVPVVHPVAARIWYVQKLLRVKNPLKGASRLLEE
                     LP"
     gene            complement(1233..1970)
                     /locus_tag="VF182_07990"
     CDS             complement(1233..1970)
                     /locus_tag="VF182_07990"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX6437057.1"
                     /translation="MSVSLNGRTALVTGSSRGIGQAIAEQLAADGAAIVINYTRNEQP
                     ARAVVNRILGKGGKAIAIQADISKAADVARLFSEAEKAMGQLDIVVANAGVYIMKPFI
                     ESTEADYDYVFGINTKGVFFTLQEAARRVRDGGRIVVISTGGTKMSFPNNSVYLGSKG
                     AIEQFARCLAVELGPRNITVNILSPGFTDTDMMPEEYHDYAASLSPFKRVGTPEDIAD
                     VASFLASDAARWVTGQNLQAGGGVISS"
     gene            2145..2444
                     /locus_tag="VF182_07995"
     CDS             2145..2444
                     /locus_tag="VF182_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX6437058.1"
                     /translation="MKQLSLTEARAKLSRLVAEVDKGNGPIAIAQRSKVKALLVDPEW
                     HDRVEEELAHYRTRSRQSPLKLRGSMTLMGDLDEALQDLKKEREQSLQGVIAALK"
     gene            2441..2842
                     /locus_tag="VF182_08000"
     CDS             2441..2842
                     /locus_tag="VF182_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX6437059.1"
                     /translation="MIYVTDTHPLVFWSSNRRRRLGNRARRILQETEQGKHSIIVPIV
                     VLEEINRLVERKIIRLDVPFRRWAEELERSPNFQVQAYALDVLLESVSLAAIRDPADR
                     AIVATARQLRCPLITADELIRDGEWVDTVWE"
BASE COUNT          783 a          885 c          891 g          699 t
ORIGIN      
        1 ttatctttgt aggtccggac ccaaatgaat tcgttctgcg gccgattcat ccaagtgcga
       61 cgatgggacc ccagggctat ttcatccaat cgtgagccga cagccaacga cagcccttga
      121 cagccccggg ccacttatag ttcagggtca ttagtggagt ttatccggct tcttccggaa
      181 ggcgtcggct gcatcccgat gtacgccggc atcaaggaac attccgaacg cggttatctc
      241 agtgcgatgg aaacctacca cgctaagatg gccgagctgg cggagatcgg cgtcgatttg
      301 atgcatcctg aaggtggccc accgttcatg gtacgcggcc ataaggcgga gcaagaaatc
      361 gtcggcggat gggaggccca atataaagtg ccgatcttca cctccggcat gtcacaggcc
      421 caggcactgc gtgccttggg tatcaaaaaa tttgtcggct gcacctacta ccgcgatcag
      481 aagatgaacg ctatctttaa ccgctatttt actgacgccg gattcgaggt tctgggcatg
      541 gagggcatgg acacgtcacc ggacgaggcg gataagattt ctaccgagac gatctatcag
      601 catctcaaag cgtcattcaa caaatttccg aaggcgcagg gcatctattt gctcggctcc
      661 ggcgcttggg gtgtcaaaga tatcattgcg gcggaaaacg atctgggcgt tccggtggtt
      721 catccggttg ccgcacggat ttggtacgtg caaaagcttt tgcgagtcaa aaacccgtta
      781 aagggagcga gccgattact ggaggaatta ccttagagag ccttacggta gccgctctag
      841 caggaaaccg tgacaggtcg gtgagccaac gatgtctggt cagtcaataa atcacaaggc
      901 aaattcaatt ctccgggggc ccgccataac ggacactggt tgacatcgga acgttgcact
      961 acaagacctc accctcattg gcatcctcgt tcgtgccctc gggcgaagcg tgcgttgggc
     1021 cgcatcggtt gggacgaatg gccccgccac tcacaggcaa gagcctgatc tcgcatccgc
     1081 caaccgacga gagggctcca tgaaacgcgc cctcatgaaa cgcatcctgt gttcgctgct
     1141 tagcgggcct gccgttcggc agcgtcctcg gtagaaaacg aatccactga cgtctgatag
     1201 ctcgaatccc gtcaatttat gcgccaaaac agctagctac tgatcacacc gcctccggcc
     1261 tggagatttt gtccagttac ccaccgcgcc gcgtcgctgg cgagaaagga cgccacgtcg
     1321 gcaatatcct ccggcgtgcc aacacgcttg aacggcgaca gactggcagc gtagtcgtgg
     1381 tactcttcgg gcatcatgtc cgtatcagtg aatcctggcg atagaatgtt caccgtaata
     1441 ttgcgcgggc caagttcgac cgccaagcat cgggcgaatt gttcgatggc tcccttgctc
     1501 ccgagataaa ctgagttgtt ggggaaactc atcttcgtac cgcctgtcga aatcacgaca
     1561 atgcggccac catcgcgcac gcggcgggcc gcttcctgca gcgtgaagaa cacgcccttg
     1621 gtgttgatgc cgaaaacata gtcgtaatcc gcttctgtgc tctcaatgaa cggtttcata
     1681 atatagacac cggcattggc aaccacgata tcgagttggc ccatcgcttt ttcggcttcg
     1741 ctgaacaatc gcgcgacgtc ggcagctttg gaaatatcgg cctgaatcgc tattgccttg
     1801 ccacccttcc caagaatcct attcaccacc gctcgtgccg gttgttcgtt gcgagtgtaa
     1861 ttgataacaa tcgccgcgcc atcggcggca agctgctcag cgatggcctg tccaatacca
     1921 cgcgagctcc cagttacgag tgcggttctt ccgttaagtg aaacagacat aaaattctcc
     1981 ttgaaagatt gtttaatcgt tcgcagggca ttgttcttct atagcaccaa ccagcagtct
     2041 gttggagaac tgtgaagtga tctagcgaag cgggcgcggc attcaccgat tgaccggcag
     2101 cgaatgcggc tgtacagtta gatgtacagc gacaggaggc gggtgtgaag caattatcgc
     2161 taaccgaagc gcgcgccaaa ttgagtcggc tggtggcgga agtcgacaag ggaaatgggc
     2221 ccatcgcgat tgcgcagcgg tcgaaggtca aggcactgct cgtcgatccc gaatggcatg
     2281 accgggtaga ggaagaactc gcccactacc gaactcggtc gaggcaatcg ccgttaaaac
     2341 tgcggggcag catgacattg atgggagatc tggacgaagc tctccaggat ctcaagaaag
     2401 aaagagagca gtccctccag ggcgtcattg ccgcgctcaa gtgatttacg ttaccgatac
     2461 gcacccgctg gttttctggt catcaaatcg tagacggcgg ttgggaaacc gcgcgcggcg
     2521 catcttgcaa gagaccgagc agggcaaaca ctccattatc gtgccgattg tcgttctgga
     2581 agaaatcaac cgactcgtag aaagaaaaat tattcgcttg gacgtgccgt ttcgtcgctg
     2641 ggccgaagag ctcgaacgct cgcccaactt tcaagtccag gcatatgcgt tggatgtttt
     2701 actggaatcg gtgtcgctcg cggcaatccg cgacccggcg gacagagcca tcgtcgcaac
     2761 ggcccgccag ctccgctgcc cgctcatcac cgccgatgaa cttattcgag atggtgagtg
     2821 ggtcgatacg gtttgggaat aagcgccgca agaatgtcct cttgcataga tcggtcggaa
     2881 gaggtgcata tttgcgtccg agttcgccca ggaaatcgcg atgaaggacg cattgcatga
     2941 catcaatgaa attctcgaac ggaaagatat caacgatcac cacaaagcca tgatactcgg
     3001 agaaaacgcc aagagattct acaacctcta gcgtgagatt ctccctctgc actgaagatg
     3061 cgagcgcgat cgattaagaa aagctgtcac gcgatcaaat aattgtgttg tcgtgacaca
     3121 agacctgacc ccgctcgatg tgacccaaaa gcttttgaga atcaaaaatc cgttagaggg
     3181 agcgagccga ttactggagg aattacctta gagagccttg cccttacccg tctagcagga
     3241 aaacgtaatc aggtcggt
//
LOCUS       DASUVS010000141         7489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1799715, whole genome shotgun sequence.
ACCESSION   DASUVS010000141 DASUVS010000000
VERSION     DASUVS010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7489
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799715"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /gene="rfbG"
                     /locus_tag="VF182_08005"
     CDS             <1..1532
                     /gene="rfbG"
                     /locus_tag="VF182_08005"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR02622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HEX6437060.1"
                     /translation="NTTKGSKLEATLLGQKHILMDVNFWQSRRVLITGHTGFKGSWLS
                     LWLQCLGAHVVGYALDPPTDPSLFDIAKVAERMVSIPGDVLNKNTLQSVIAKHQPEVI
                     FHLAAQSLVRESYKHPVETFSTNVMGLVHLLEGVRTVGGVRAVVCVTSDKCYQNREWV
                     WGYREDEPVGGYDPYSSSKGCSELVAAAYRNSFFNEREYSRHEVAVATARAGNVIGGG
                     DWAKDRLIPDLVRSVIHGQDIVLRNPHAIRPWQHVLDPLAGYLMLAESLFRHGPRFSE
                     AWNFAPAESHTVTQVVGLLLSLSGSEVSAKHDQTRQPHEDPYMILDSTKARVKLDWKP
                     LLDLRTSLSWILAWVKRLQAGIDMRSVTESQIKAYMDLAQSSKSADHARDRAPAPRND
                     SHLLDLVQETIMIRDREGRIQYWNRAADEMYGWKSEEVIGNISHEILRTKFPKSLSEI
                     ERELAKEGRWQGELVHTRRNGSRIVVKSRWVLKHDDRDTGGEVIEINQICTDQEQNLR
                     Y"
     gene            1586..2122
                     /gene="rfbC"
                     /locus_tag="VF182_08010"
     CDS             1586..2122
                     /gene="rfbC"
                     /locus_tag="VF182_08010"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015132743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEX6437061.1"
                     /translation="MIFNETKLKGAYIIQLEPSEDERGSFARMFCREEFETHGLNSAV
                     VQCNISKNRRKGTLRGMHYQVAPHAEAKLVTCLAGSIYDVIIDLRPGSITCCRWIAVE
                     LSARDPRSLLYIPEGFAHGFQTLEDDTEVFYQMSHAYVPEAARGVRWDDPAFAIQWPD
                     GPRTISTRDLHYPDFRPA"
     gene            2302..3567
                     /locus_tag="VF182_08015"
     CDS             2302..3567
                     /locus_tag="VF182_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HEX6437062.1"
                     /translation="MYRLVTELFPICRSITGKGVRETLALVQERVPIDIREVPSGTRV
                     FDWIVPKEWNIREAWVKDPAGKKIIDLQKSNLHVLNYSAPIHATLSLGELKHHLYSSP
                     EHPDWIPYRTSYYNNNWGFCLPHNQLLGLSEGDYEVFIDSSLEDGHLTYGEHYLRGET
                     EVEVLISAHICHPSLANDNLSGIAVAAFLADHLSKLPLKYSYRFLFIPGTIGSITWLS
                     LNESEVSRIKHGLVFTCLGDSGKFTYKKSRRGDTEIDRAATEVLKNSGEAFEVREFSP
                     YGYDERQFCSPGFNLPVGCLMRTPHAQYPEYHTSADNLDFVRPEYLADSFTKCLAIIE
                     ILERDRVYWNLNPKCEPQLGKRGIYSQVGGQTDGKSDELALLWVLNLSDGKNSLLDIA
                     ERSNLKFSAIANAAQVLFDKGLLRQDRST"
     gene            3722..5647
                     /locus_tag="VF182_08020"
     CDS             3722..5647
                     /locus_tag="VF182_08020"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6437063.1"
                     /translation="MISLHRSQSPLESATDSVMTRTVEGIINFWNRAAEDLYGWKKEE
                     AIGKVSHDLLKTKFPKPLEEIESELVTQGCWEGKLVHSTRDGGNVVVESRWILDHSRK
                     LGAVVEINAPYEAGKAKTNVGSTSSSGSRRISVFPTSGLTARRRDHTLWVLSYVGLIS
                     VALIWILYSVFAHPLIQDMYHEQAVIPFLNQIMEGRDVTQIETYHQAADRLMLSGTLW
                     LITSYLIVLFVVKYPVGALLSVISFVVSSFFFFSLFEFYPSLIKPLGLDAISYYAHKV
                     NYIDDDVLIYREKPFNNVNYENYRSENYSALYGIEVAPVHFQWITDKNGFRNGHAREY
                     SDIVAIGDSYLEWGNSEAYTFVKMLENKLTGVTVTNLGKSGYGPAQYLEVLSRYGLDY
                     RPKYVLMAFFEGNDVQDTKSYFRWKTGKTERLPNFPYRMAQLSFLPRYRLALETQATF
                     ISSTIRYWIDLALNRIAQHNGYAYEIHPDLALLNLGKGETRKIKFVEELDTRSPKELL
                     ATEEWQQLKTILSSARHLSQNNGSSLILMYIPAAAHIYAQYSTEQSGEHWLKIRDQQI
                     AAKNNTESAMKQIANELNIDLISLTPVLEEAARNGKLLYYPLDPHWNAEGTELAASFV
                     AESLKSKYLLGSGAGNR"
     gene            5804..6700
                     /locus_tag="VF182_08025"
     CDS             5804..6700
                     /locus_tag="VF182_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437064.1"
                     /translation="MSRVREWLQEESVEQTAASENKPRYEWLNDQYLNVAQQIRGKPH
                     YAWGVVQAAGLAQVLGLRHISVIEFGVAGGNGLVALQDIAARAEALLEIKIDVYGFDT
                     GVGLPKPTDYRDMPHFFAEGFYPMDSAKLHSRLHKGILRLGEIKKTISEFIDSKPAPV
                     GFISFDLDFYSSTKDAFALFEAEPNLLLPRIYCYFDDILGFSYSEFTGELLAISEFNA
                     GHAMRKISKIQGLAHFLPPGPPRMWAEQFYLAHIFDHESYGRYDGLNPARNLDLSVNS
                     VRPTQEKKWSTEQISNELTVKR"
     gene            6739..>7489
                     /locus_tag="VF182_08030"
     CDS             6739..>7489
                     /locus_tag="VF182_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006539844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437065.1"
                     /translation="MNAIYFDSPLSETVRRRLLFEGDLFIFSPRPASLALCQFADELI
                     RKAFGRLSPEKAQYSLPVEEYAAILSKLKPEFINHPKSKECIQAIVAEFDCEPKVTYF
                     DVPRMRTSTSDGYLTTGIAYAWHPHRDTWYSAPQCQINWWIPIYGIDTNHGVAFHPRY
                     WKQPIKNDSKRYNYYEWNKKFRPDASKHLKEDPRPLPRPTEAVESEPYISPVCPVGGI
                     IMFAGAQLHSSMPNSSGKTRFSIDFRTVNHAD"
BASE COUNT         1903 a         1798 c         1911 g         1877 t
ORIGIN      
        1 gaaatactac caaaggatcc aaacttgaag cgactttgtt ggggcaaaaa catattctta
       61 tggacgtcaa tttttggcaa tcaagaaggg ttcttataac cgggcatacc gggttcaaag
      121 gcagctggct gtcactctgg cttcagtgct tgggcgccca tgtggttggt tatgcgcttg
      181 accctccaac cgatccaagt ctcttcgaca tcgcgaaagt agccgagagg atggtttcga
      241 tcccgggtga cgtgctgaat aagaacactt tgcagtcggt catcgcaaaa catcagcctg
      301 aagtgatttt tcacttggct gctcaatcgc tcgtccgtga atcctataag catccggttg
      361 agaccttttc gaccaacgtg atgggactag tgcacttgtt ggaaggtgtt cgaaccgttg
      421 gtggggtgcg agcggttgtc tgcgtcacat cggataaatg ctatcaaaat cgcgaatggg
      481 tttggggcta tcgagaagat gagccggtgg gcggttatga cccgtacagc agcagcaagg
      541 gctgctcgga attagtcgct gcggcgtatc gaaattcgtt tttcaatgag cgtgaatatt
      601 cacggcacga ggtggcggtt gcaacagccc gcgccggaaa tgttatcgga ggcggcgatt
      661 gggcaaaaga tcgcttgatt ccggacctag tacgatcggt gatccatggt caggacatcg
      721 tattgaggaa ccctcatgct atccggcctt ggcaacacgt gctcgatccg cttgccggat
      781 acttgatgct tgcggaatct cttttcaggc acgggccacg tttcagcgaa gcgtggaact
      841 ttgcaccggc ggaatcccat acagtgaccc aggtggttgg actactgctc tcgctatcgg
      901 gcagcgaggt ttctgccaag cacgaccaga ctcgtcaacc gcacgaagat ccatacatga
      961 ttttggacag taccaaggcg cgggtaaagt tggactggaa accgctatta gacttacgca
     1021 cgagtttaag ttggattctc gcctgggtta agcgcctgca agcgggcatc gatatgcgca
     1081 gcgttacaga gtcgcaaatt aaagcttaca tggaccttgc tcaaagtagc aaaagtgcgg
     1141 atcatgcccg agatcgcgcg ccggctcctc gcaacgactc tcaccttctc gatttggttc
     1201 aggaaacgat tatgattcgc gatagggaag gcaggattca gtattggaat cgggccgcag
     1261 acgagatgta tggttggaag agcgaggaag tcataggtaa tatttctcat gaaattcttc
     1321 gaacaaaatt tcccaagtcg ttgagcgaga ttgagcgcga gctcgccaaa gaaggacgtt
     1381 ggcaaggaga actcgtgcac acccggagga acggcagccg tattgtggta aagagtcgct
     1441 gggtgttgaa gcacgacgat cgagataccg gtggtgaagt catcgaaatc aatcaaatct
     1501 gcacagacca agagcagaat ttaagatatt aaattgggtt gagacgctca ggaccgtgtt
     1561 ccgtttgccc gctatgtcat agctcgtgat ctttaacgaa accaagctca aaggcgctta
     1621 tatcatccaa cttgaacctt ccgaggacga acgcggatct ttcgcccgta tgttctgtcg
     1681 tgaagaattc gagacacatg ggctaaactc ggcggtcgtc caatgcaaca tctcgaaaaa
     1741 tcgtcgaaag ggcacgcttc gcggcatgca ttatcaggtc gcgcctcatg ccgaggcgaa
     1801 gcttgtgacg tgtctcgccg gctctatcta cgatgtcatt atcgatttaa gaccgggttc
     1861 aatcacctgc tgccgctgga tagcggtgga actcagcgcg cgggatccgc gtagcctcct
     1921 ctatataccg gaaggttttg cgcacggttt tcaaacactg gaagatgata ccgaggtttt
     1981 ttaccagatg tcacacgctt atgtgcctga agccgctcgc ggcgtgcgtt gggatgatcc
     2041 ggcgtttgcg atccagtggc cggatggtcc tcgaacaata tcgacgcgcg acctgcatta
     2101 tccggatttt cgcccggcct aatgggcatc ccgacttcgt gcctctgtct tttgcaattc
     2161 taggcgccta gatcgcccct tcggccaatc ggattcaaca gcaacactgc tcgtagtcac
     2221 ttcatgtcag tacctgaaac agccataaaa aaaagttcaa tctcttcacc ggatcttgat
     2281 agagccaaaa taggtgagga gatgtatcgc ttggtgactg agctgtttcc gatctgccga
     2341 agcatcaccg ggaaaggcgt gcgtgaaaca ctggcgcttg ttcaagaacg cgttcccatt
     2401 gacattcgcg aagttcctag cggcacaaga gttttcgatt ggatcgttcc gaaggaatgg
     2461 aatatccgag aagcttgggt caaggatcca gcaggaaaaa aaattatcga tttacaaaaa
     2521 tcgaaccttc atgtgttgaa ctacagcgcg ccgattcacg ctacgttatc gctcggggaa
     2581 ctcaaacatc atctttactc gtcgccggaa caccctgatt ggattccgta tcgaacctct
     2641 tattataaca acaactgggg gttctgcctg ccgcacaacc agcttctggg tctttcagaa
     2701 ggtgattatg aggtatttat cgactcatcc ctggaagacg gccatctcac ttatggcgaa
     2761 cattatctca ggggcgagac agaagtggaa gtactaatat cggcgcatat ttgccaccca
     2821 tcgttggcca atgataatct ttccggaatc gctgtcgcag cttttctagc agatcattta
     2881 agcaaacttc cattgaagta ctcctaccga tttctgttta ttccgggaac tatagggtcg
     2941 atcacctggc tgagcttgaa cgagtctgaa gtctcccgga tcaaacatgg tctcgtattc
     3001 acctgtctcg gcgattctgg gaagtttacc tacaaaaaaa gcaggcgggg cgatacagag
     3061 atagaccggg cggcaaccga ggttctcaag aactcgggtg aggctttcga ggtccgtgaa
     3121 ttttcgcctt acggctacga tgaaaggcag ttctgctctc ccggcttcaa ccttcccgta
     3181 ggctgcttga tgagaacgcc gcacgcgcaa tatcctgagt accatacatc ggccgataat
     3241 ctagattttg tacggcccga gtacttggcc gattctttca cgaagtgctt ggccataatc
     3301 gaaattctgg agcgtgatcg cgtgtactgg aatctcaatc ctaaatgcga gccgcaatta
     3361 ggaaagaggg gcatctacag tcaagtcggt gggcagacgg acggcaagag cgatgaattg
     3421 gccttactgt gggttttgaa tttatctgac ggaaaaaaca gtcttctcga tattgcagaa
     3481 agatcaaact tgaagttttc ggctatcgcc aatgcagccc aagtcctttt cgataagggc
     3541 ttattacggc aagatcgtag cacatagcac atactcctct gctattctct taccaacttt
     3601 ggacctacat acgttaccaa atcctatttc cgggacaaag tatcggcgat ttcatcttcc
     3661 ttatctggca cataggttgc tgttttatcc ctgatgagat gtaggaatgc ctggagcaaa
     3721 catgatttcg ttacatcgca gtcaatctcc tcttgaatct gcgaccgaca gcgtgatgac
     3781 gcgcactgtc gaggggataa taaacttttg gaaccgtgcg gctgaagatc tctatggttg
     3841 gaaaaaggag gaagccatcg gcaaagtcag ccatgacctt ttaaaaacga agtttcctaa
     3901 accattagaa gaaatcgaat ccgaacttgt cactcagggg tgctgggaag ggaaactcgt
     3961 acattcgact cgggatggcg gtaatgtcgt ggtggagagc cgatggattc tggatcatag
     4021 ccggaagctg ggcgcggtgg tcgaaataaa cgcgccgtat gaagcaggca aggctaaaac
     4081 aaacgtcggc tcgactagct caagcggctc aaggcggatt agtgtatttc cgacctcagg
     4141 actgacagcg cgccgccgag atcataccct ttgggtatta agctatgtcg gcttaatttc
     4201 tgtcgctctc atatggattc tctatagtgt gtttgcccac cctttgattc aggacatgta
     4261 tcacgagcaa gcggtcatac ctttcttaaa ccagatcatg gaaggccgag acgtcacaca
     4321 gatcgagacc tatcaccagg cggccgacag gctcatgctg tccggaaccc tatggctcat
     4381 tacgagctat ttgatcgtac tatttgtggt gaagtatccg gtgggcgctc tgttgtcagt
     4441 gatctccttt gttgtttcgt cgtttttctt ttttagcctt ttcgagttct atccctccct
     4501 gatcaaacca ttaggcctgg atgcgatctc ttattatgct cacaaggtta actatatcga
     4561 cgacgatgtg cttatttatc gagaaaagcc ttttaataac gttaactacg agaattaccg
     4621 gagcgaaaat tattcggcgc tctatgggat agaggtggca ccggtccact tccagtggat
     4681 tactgacaaa aacggtttta gaaatggcca tgcccgggaa tactctgata ttgtcgccat
     4741 cggcgattcg tatctcgaat gggggaacag cgaagcttac accttcgtca aaatgcttga
     4801 gaacaagctg acgggggtaa cggtcacgaa tttgggaaaa tccggatatg gtccggctca
     4861 ataccttgaa gtgctgagcc ggtatggtct ggactaccga cctaagtacg tattgatggc
     4921 ctttttcgag ggaaacgacg ttcaggatac taagtcctac tttcgttgga agacggggaa
     4981 aaccgagaga ctgccgaatt ttccatatcg aatggcgcag ctttcttttc taccgcgtta
     5041 tcggctggcg ctggaaacac aggcgacatt tatctccagt acgattcgct attggatcga
     5101 cctcgctctg aaccgaatcg ctcaacataa tggctacgcc tatgagattc atccagactt
     5161 ggcacttctg aatcttggga agggcgaaac ccgtaaaatc aagttcgtcg aagaactcga
     5221 cactcgatcg cccaaggaat tgttggctac tgaggaatgg caacaattaa aaacaatcct
     5281 gagcagcgcg cgacacctta gtcaaaataa cggttcttct cttattttga tgtacattcc
     5341 cgccgctgcg catatttatg cccagtattc taccgaacaa agtggagagc actggctcaa
     5401 gattcgtgat caacagatcg ccgccaagaa caatacagaa tcggccatga agcagatcgc
     5461 aaatgaattg aatatcgatc tcataagctt aactccagta ctcgaagagg cggcgcgaaa
     5521 tggaaagctg ctgtattatc ctctggaccc acattggaat gccgaaggga cagagttagc
     5581 tgccagcttc gttgccgaat cgctcaagtc aaagtacctc ttgggctccg gggccgggaa
     5641 tcgctgaacg gcgtttcttg gctgaattct gtccttccag aatctctaca ttacagcggt
     5701 cgcagtactt atgttttcag catttgtata ggaagaatgt taatcgtatc gacgggtaac
     5761 agatatgatc aaacgcgtgt taaagaggtt gataagaggt ggagtgagta gagtcaggga
     5821 atggctacag gaagaatcgg ttgagcaaac ggcggctagt gaaaacaaac ccagatacga
     5881 gtggttgaac gaccaatatc tgaacgtcgc gcaacagatt agaggtaagc cgcattatgc
     5941 ttggggagta gttcaggcgg ctggactggc acaagtttta gggcttcgcc atatctcagt
     6001 gattgagttc ggtgtcgctg gcggcaacgg gctagtcgca ttgcaagaca tagctgcccg
     6061 cgccgaagct ttgttggaga tcaaaatcga tgtctacgga tttgacaccg gcgttggctt
     6121 gccgaagccg accgactacc gagacatgcc gcatttcttt gccgaagggt tttatccgat
     6181 ggattcggct aagcttcatt cgcgattgca taagggaatc cttcgtttag gtgagattaa
     6241 gaagacgata tcggagttca tcgattcgaa gccggcgcct gttggcttca tctctttcga
     6301 cttggatttc tacagctcga ctaaggacgc ctttgctctt tttgaagcgg agccgaatct
     6361 cctcctgccg cgcatttact gctacttcga tgacattcta ggtttctcat acagtgagtt
     6421 tacgggtgag cttctggcta tttccgagtt taatgccggt catgcaatga gaaagatttc
     6481 caagattcag ggactagcgc acttcttgcc cccggggccg cctaggatgt gggccgaaca
     6541 gttttatttg gcacatatat ttgaccatga atcctacggc cgttatgatg gcctaaatcc
     6601 cgccagaaac ctggatttat ccgtcaatag cgttcggcca actcaggaaa aaaaatggtc
     6661 caccgagcag atttctaacg aattgacagt caagcgttag ttttcagact attcggtgca
     6721 atcgcaccag gaggtagtat gaatgcgatt tactttgact caccgttgag tgaaacggta
     6781 agacggcggc ttctgttcga gggcgatcta tttattttct ctcctcgccc ggcgtcgctc
     6841 gcactctgcc agtttgctga tgagctaatt cgaaaagcat tcgggaggct ctctcccgaa
     6901 aaggcgcagt acagtctgcc ggtcgaagaa tatgctgcga tactctccaa gctaaaaccc
     6961 gaatttatta atcatcctaa atccaaggag tgcatccaag cgattgtggc ggaatttgac
     7021 tgtgaaccca aagtcaccta cttcgatgtt ccccgaatga gaacctcgac gagcgacggc
     7081 tatctgacga ccgggatagc ttatgcttgg cacccccacc gggatacatg gtattccgca
     7141 ccgcaatgcc agatcaactg gtggattcca atctatggca ttgataccaa tcacggagtt
     7201 gcattccatc cacgctattg gaagcagcca ataaaaaatg attcaaagag atacaactat
     7261 tatgagtgga acaaaaaatt caggcctgac gcatcgaagc atcttaagga ggatccgcgt
     7321 ccactaccgc gcccgacgga agccgtcgaa tctgaaccat acataagtcc agtttgccca
     7381 gtcggcggca taatcatgtt tgccggtgcc caactgcatt cctcaatgcc aaatagttcg
     7441 ggtaaaacgc gcttcagcat tgattttaga actgtgaacc atgccgacc
//
LOCUS       DASUVS010000142        13976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_707774, whole genome shotgun sequence.
ACCESSION   DASUVS010000142 DASUVS010000000
VERSION     DASUVS010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13976
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707774"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VF182_08035"
     CDS             <1..762
                     /locus_tag="VF182_08035"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX6437066.1"
                     /translation="AGIIKKGVPVVCGALPLEAMEVIQQIADSMQAAAYRLGQNFTLK
                     LKTNGSFDYNGANWNFPDLSVALRGAHQIRNAGVATAALEVVYDRFPTSELAIRSGLQ
                     NVSWPGRMEMILTDPAVIIDGAHNREGFEVLADELRQLRVGRRIRMLFAVMDDKDWRF
                     MLDILAGVVDEIVFTRVSQLERSADPVRLQQELAGRLQSQVIPDPRSAVETLIQSAGP
                     NELIVITGSLYLLGEVRPLLTTMAQARQSDQTNLN"
     gene            782..3103
                     /gene="lptD"
                     /locus_tag="VF182_08040"
     CDS             782..3103
                     /gene="lptD"
                     /locus_tag="VF182_08040"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX6437067.1"
                     /translation="MKPPKVLLIILALCCWLPGPGNGQAQTTKPSSGADTQINVTADK
                     LSISESGAQIEASGNVEIERQGTTLKAEQINVNRTTQDVQATGKISLDDPEWKINSAD
                     SISLNLEKETGEMTNADLFIEQGHISISGSRFQKLGGQTYHVDEGFFTTCLCDTGPVP
                     WRFSAEQIDLTAGGLGTVKNGYFYIYDVPVFYLPYGFFPLKTERQTGFLFPKFGTSTD
                     EGFRFQQSFFWAISKSTDTTFAFDFQTDARYGFLNEIRTLFDRDSDFFLQTAYFNEGL
                     RQREQGDVVDRTIADPDIPVNRWGIMGTHRYDFASNWLTYSDIAAYRDDLFTRELVER
                     FDLAPTVQGDIRRSRYASSRFGVFKSWGDSHFQGEWNFYQDFIQPDAITLHRTPQISF
                     WGRKGLENFPLEMRWRAEGVNYIRREGGDGMRLDLRPELLLPLRMSSLFGSLSIAPRQ
                     TLYHLYETVQSSDHNVSRELVEIRGNIGTAIGRVFQWNALGLSRFKHVIEPELSYLFV
                     PGIDQTRIPIMDGIDRVRRRNVVTFGFNNRFSGKSLSPLVDPESLRQVEPLNVAMGGA
                     AQDLGSLRFALSYDVDKERKGGDTLSDLDMNLTLNLTNFLSFNFDGGVDPGRWDVTQA
                     RANVSLTDPRTMTRLYLDPDFNRPNSFNIGYHYLSRGINGFLAENANIDCIDNPFDPA
                     CPSVVNKTTVGNLVLGGLYHLTEHLLTHVNTTYDVRETRFLNFRAAAKLLSRCECWTL
                     TLGLQRDINPAKTTFNFTFGLLGLGGSEQSSLR"
     gene            3426..4364
                     /locus_tag="VF182_08045"
     CDS             3426..4364
                     /locus_tag="VF182_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6437068.1"
                     /translation="MPTHVVVTGAAGYLGSILCEHLLAAGYRVTAADGLIYRQRTLFH
                     LCANPLFDFVVGDARDEQLMRRLVKDADVLIPLAAIVGAPACDRDPWLARSVNLEAIR
                     LLARLRSNDQQIVYPTTNSGYGTKSGDVFCTEDTPLEPISLYGQTKTEAESEILERTN
                     TVTLRLATVFGMSPRMRLDLLVNHFVYAAVTDCYIVIFEKDFKRNYIHIRDVADCFIH
                     CIKNGKKMAGRPYNAGLDSANLSKEALALKIKEYVPNFYIHFGQVGTDPDKRNYIVSN
                     QRLRDAGFEAKRSLDSGIHELLKGYRMLGRAEFANI"
     gene            4390..5145
                     /locus_tag="VF182_08050"
     CDS             4390..5145
                     /locus_tag="VF182_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX6437069.1"
                     /translation="MGQDRSAFSALSVAILAGGLGTRLLPVVSDRPKALAEIHGRPFL
                     AYLLGQLADAGSSRVVLCTGHLGEQIEQAFGERYRTLQIFYSRERRPLGTGGALRLAL
                     PYLLSDPVLVMNGDSFCATDLTSFWHWHASRGSRATMLLAEVPNTQRYGSVKINHDGA
                     VTQFAEKRQGGPGLINAGVYLLSRRVIDSIPEDTPVSLEHNLFPALMNQGLYGYQERG
                     RFLDIGTPEDFAAAEEFFAPINERETTAVCSTR"
     gene            5114..5674
                     /locus_tag="VF182_08055"
     CDS             5114..5674
                     /locus_tag="VF182_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6437070.1"
                     /translation="MKRRRFVVLDRDGTIIEEVPYLSDPRQIALIPGTGKALRELSEM
                     GFGLVVITNQSAVGRRYFDEAQLARIHDRLLEMLRAEQITLDGLYFCPHTPQDQCLCR
                     KPNTGLIEKASEDLEFDLRGSIVIGDKASDIEMGCRVGALTMLVRTGYGAQVAAEQKV
                     EPDYIVDDLPAAVQVIDTICRKVRHQ"
     gene            5671..6744
                     /locus_tag="VF182_08060"
     CDS             5671..6744
                     /locus_tag="VF182_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEX6437071.1"
                     /translation="MIISRTPFRISFFGGGTDYPAWYRDHGGTVLGATIDKYCYLTCR
                     YLPPFFKHRIRVVYSQIENCQAVDEIAHPAVREVLRYLQIDRGVEIHHDGDLPARSGM
                     GSSSAFTVGLLHALYALKGQMPTKRQLAAESIQIEQEMIKETVGSQDQVLAAFGGFNH
                     VVFSQDGEITVKPITLSGDRLRELSSHLMLFYTGIERTASDIAGSYVTDVDGKRSQLR
                     IMKGLVEESLAILNNGQDITAFGALLDEGWKAKRSLSARVSNSYVDELYAHATANGAL
                     GGKLLGAGGGGFMLLFAPPNKQNQVKEALKRFISVPFHFDFSGSQIIFFDHEKDYSAE
                     EKIRDSQEYHFVGELNDRLDRDL"
     gene            6792..7727
                     /locus_tag="VF182_08065"
     CDS             6792..7727
                     /locus_tag="VF182_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEX6437072.1"
                     /translation="MHKDARIFIADEHGLIGEVLKRRLRDDGYQNLLGDSGNPSLIDA
                     REVEAFFLRTLPEFVFAVGGKSGGIGANQKLPADLMLNNLHVDCHVIDSAYRCGVKKL
                     LYMGSSCCYPRDCLQPMQVSSLMTGVLESTNEAYATAKLAGIKLTQAYRRQHGADMIS
                     AIPANVFGPGDDFGVEDSHVIAALMAKMHQAKFSQTQRVEVWGSGLPRREFVYVTDIA
                     DAAIFLMKHYSSPVPINVGSGVDASIREIAELIKDVVGFSGELCFDPKKPDGMPLKSL
                     DSSELFALGWQPKIPLRLGLEKTYQWYLETRHTNA"
     gene            7732..8670
                     /locus_tag="VF182_08070"
     CDS             7732..8670
                     /locus_tag="VF182_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010047630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX6437073.1"
                     /translation="MNERIFKSLYRIRRVEEEIARIYPSDKIKSPVHLSIGQEWIAVG
                     VCEALRPEDTVFGSYRSHAYYLAKGGDLKKMFAELFGKATGCAKGKGGSMHLVDVGHG
                     VMGASAVVGTTIPNAVGYAYAMKLQKSKNVVVSFFGDGATEEGVFYESLNFAALKELP
                     VIFVCENNFYAIHTHISRRQKTPNPCDKAKAQGIVAEQIGDGDMRRLYERVGAAADLM
                     RAGRSGPYFFECLTYRWKEHVGPNEDFDLGYRSRQEASPWVERDALPRIECLLTAEAC
                     SRLKTEVEEEIRAAIRFAEESPYPEPAELFMDVIGD"
     gene            8671..9717
                     /locus_tag="VF182_08075"
     CDS             8671..9717
                     /locus_tag="VF182_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6437074.1"
                     /translation="MERTLSYIDAIREATDQEMARDGSVVIFGLDVDDPKAIQGTTRG
                     LLEKYGPERVFGTPLSEDAMTGVAIGMALAGVRPIHVHIRMDFMLLAMNQLVNIAAKS
                     RYMYGGQVHVPMVVRCMIGKSWGQGAQHSQGLQSLFMHIPGLKVVAPANPYDAKGCLI
                     QAIRDGDPVIYVEHRILHFQKGLVPESEYGVPAGKARVLAEGEDITLVGVSYMVLECL
                     RARDYLASTGIDAEVIDPVWLSPLDIDTIVKSARKTGRLCVVDNGWTACGAGAEIVSQ
                     VIERVQHDRHVAVQRLGYAPVTCPPTPVLEDLYYPNARTIASSAYDLVKGGKNAWLPQ
                     ERKDLAEFEFRGPF"
     gene            9758..10936
                     /locus_tag="VF182_08080"
     CDS             9758..10936
                     /locus_tag="VF182_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEX6437075.1"
                     /translation="MKNNLAKEDFAKVVELLHREDPILTQSDQVKEFEKEWSAWLGVK
                     HSVFVNSGSSANLLTMCALKERYGSGEIIVPPLTWVSDIASVIQSGFKPVFVDIDRRT
                     LGMDHQQILEKISPNTRAVFMTHVLGFNALKKSLVAELEGRGIPLIEDVCESHGATCE
                     GRKLGTFGLISNFSFYYAHHMSTIEGGMICTNDADLYEILRMLRSHGMVRESTSSELK
                     ENFYRNYPDLNPDFIFAFPAYNVRSTEINAVIGRSQLKRLDANNKIRSENLTLFLSHL
                     HPEKYQTDFATAGSCNYAFTLVLNRADPVLCENVMQMMRRCNVEFRRGTSGGGNQLRQ
                     PYLRRIMGERDFEKYPNVDHVHFYGFYIGNYPDLEQEKITQLCSLLNSVSGEEARYVS
                     "
     gene            10926..12440
                     /locus_tag="VF182_08085"
     CDS             10926..12440
                     /locus_tag="VF182_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010047624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6437076.1"
                     /translation="MSRNGQLDLVLVHPGTRMQVYQSLGSKLAAIEPPVWASLLATFV
                     RKHGFSVQILDAEADNLTPQETATRIEEMDPRLAVVVVYGHQPSASTQNMTVSGLICA
                     ALKELNGDRKLALVGGHVAALPERTLREEAADFVCTGEGPYTVLDLLEAGKATSPDYS
                     KVRGICYWKDGVAMSTPAAPLVKNLDEEIPELAWDLLPMEKYRAHNWHCFGDLQREPY
                     ASFYTTFGCPFHCSFCCIQAPFKTGEKEAGWKESVNSYRFWSAQAVIAQIDKLVNQYG
                     IRNIKIADEMFVLNSRHILGICDAIIERGYDLNIWAYARVDTVKDGMLEKLKRAGFNW
                     LAFGIEAASDRVRDDVDKGFDQEEIFRTLGKVRATDVNVIANYIFGLPEDDLESMQST
                     LNLALELNCEFANFYSAMAYPGSQLYNLAVKEGWPLPEKWSGYSQHSVDTLPLPTKHL
                     SGAEVLRFRDQAFQRYFSSPQYLRMMEAKYGPATAEHIREMAAQPLVRKILAAA"
     gene            12468..13088
                     /locus_tag="VF182_08090"
     CDS             12468..13088
                     /locus_tag="VF182_08090"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6437077.1"
                     /translation="MRKRRSADSTGAKSQHPEREARTLACENSQFHIFFDELSLKGRT
                     TITDYLVVAPKRVAADLVTGVAILPVFEGKIGLLWIYRHPIGRYAWEIPRGFIDQQEV
                     SATAASRELKEETGLDCKPEAIRSLGFITPDAGILAARVQLFAATQCEKERDFAPAEI
                     GHRELRFFNMEQVEKMILVSELQDPSTIIAFFLYSRFDNQVPDGSV"
     gene            13085..13789
                     /locus_tag="VF182_08095"
     CDS             13085..13789
                     /locus_tag="VF182_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6437078.1"
                     /translation="MKVTLLVLALNEIEGMKAIMPRVKKEWYDQIIILDGGSTDGTIE
                     YAKKQGYFVYVQKRRGFRHAYTEVLPYIEGDVIVTFSPDGNSVPERIPALIEKMNEGY
                     DMVIGSRYLDGAKSDDDDVITAFGNWLFTKTINVLHGGCYTDSMVIFRAYKTQLIYDL
                     ELHTDEAYSAAEKLFHTTISWEPLLSVRAAKRRLKVGEIPADEPPRIGGQRKLRVIQW
                     GGAYYFQFLREKLFWR"
BASE COUNT         3338 a         3461 c         3832 g         3345 t
ORIGIN      
        1 gcgggcatca tcaaaaaagg agtcccggtc gtctgtggcg ctctgccgct cgaagctatg
       61 gaagtaatcc agcaaatagc cgatagcatg caagcggcag cttaccggtt gggccaaaac
      121 ttcactttaa agttgaaaac taacggctct ttcgattata acggggcgaa ctggaatttt
      181 ccggatcttt cagtggcgtt gcgtggagcg catcaaattc gtaacgccgg agttgccact
      241 gccgcgctgg aggttgtgta cgatcgattt cccaccagtg aactagcaat acgttccggt
      301 ttgcaaaatg tatcatggcc tggacggatg gaaatgattt tgacggatcc ggccgtgatc
      361 attgatggcg cacataatag ggaaggcttc gaggttttag ccgatgagct tcggcagcta
      421 cgagtcggcc ggcgaataag aatgttgttc gccgtaatgg acgataaaga ctggcgattt
      481 atgctcgata tcttggcagg cgtggtcgat gaaatcgtct ttactcgagt cagtcaactg
      541 gaaaggagcg ctgacccagt tcggttgcaa caggaattgg ctggtagact gcagtctcag
      601 gttatcccgg atccccgttc ggctgtcgaa actctcatcc aaagcgccgg acccaatgaa
      661 ctgatcgtca tcacagggtc gctttacctg cttggcgaag tacgccctct gctcacaacc
      721 atggcacagg caagacaatc ggaccagacc aacctcaact gagtcctccc gggcgtagaa
      781 aatgaagcca cccaaggttc ttctcatcat tcttgcactc tgctgttggt tgccgggccc
      841 aggtaacggg caggcgcaaa caaccaaacc gagtagcggc gcggatactc aaatcaacgt
      901 aactgctgat aagttatcga tcagcgaaag cggcgctcag atagaggcca gcggcaacgt
      961 cgagatcgag cgtcaaggaa ccacacttaa ggccgagcaa atcaacgtta accggaccac
     1021 tcaagacgtc caagcgacag gaaagatttc gttggacgat cccgagtgga agataaattc
     1081 cgcagactcc ataagcctaa acctggaaaa agaaaccggg gaaatgacca atgccgattt
     1141 gtttatcgaa cagggacaca ttagcatcag cggcagccgg tttcagaaat tgggcggcca
     1201 gacctatcac gtcgacgagg ggttcttcac cacgtgtctg tgcgatacgg gccccgtgcc
     1261 ttggaggttt tcagccgagc aaatcgacct gacagcggga ggcctgggca ccgtcaaaaa
     1321 cggctatttc tacatctatg atgtccctgt cttttatctg ccttatggat tttttccgct
     1381 aaaaaccgag cggcaaaccg ggtttctgtt cccgaaattc ggcacttcaa ctgacgaagg
     1441 atttcgcttc cagcagtctt tcttctgggc tatctcgaaa agcaccgaca ccacgtttgc
     1501 gttcgatttc cagaccgatg cacgttatgg gtttttaaac gaaatccgca cgcttttcga
     1561 tcgtgattcg gattttttct tacaaaccgc ttatttcaac gaaggattgc gccagcgcga
     1621 gcagggagac gttgtcgatc gaacgatcgc cgaccccgat attccggtca atcgctgggg
     1681 aatcatggga acgcaccgtt atgactttgc ctcgaactgg cttacatata gcgacatcgc
     1741 tgcttatcgg gacgatttat ttaccagaga attggtggag cgcttcgatt tagcgccaac
     1801 cgttcaaggc gatatccgga gaagccgtta tgccagttcc agattcgggg tcttcaagag
     1861 ctggggagac agtcattttc aaggcgaatg gaatttttac caagatttta tccaaccgga
     1921 tgccatcact ctgcatcgaa cgccacagat ctccttctgg ggaaggaagg gtttagagaa
     1981 tttcccgttg gaaatgcgct ggcgcgccga aggggtgaac tatatcagga gagaaggcgg
     2041 cgatggcatg cgcttagatc ttcggccgga actcctgctg ccgctgcgaa tgtcatcttt
     2101 attcggctcg ctgtcgattg cgccgcgcca gacgctctac catctctatg agaccgtcca
     2161 gtcttcggat cacaacgttt cccgcgagct tgttgagatc cgcggcaaca tcggtacggc
     2221 gatcggccgg gtgtttcaat ggaatgcctt gggactgagc cgatttaagc atgtgattga
     2281 gcctgaacta agctacctgt ttgtccctgg catagatcag actcgaattc caatcatgga
     2341 cggcatcgat cgggttcgtc gacgaaatgt tgtgacattc ggtttcaaca atcggttctc
     2401 gggtaaatct ctaagcccat tagtagatcc cgaatccctt cgacaggtcg aaccccttaa
     2461 cgttgccatg ggcggcgcgg cgcaagacct gggctctttg aggttcgccc taagctacga
     2521 cgtcgacaaa gagcgcaaag gcggcgacac cctctcggat ctggacatga atttgactct
     2581 caatctaaca aactttctta gttttaactt cgatggcgga gtcgatccgg gaagatggga
     2641 cgtaacccaa gcgcgggcga atgtctcgct taccgatcct cgaaccatga cgcgcctcta
     2701 tttagatcca gatttcaacc ggccgaattc tttcaatatc ggataccatt acttaagtcg
     2761 cggtatcaac ggttttctgg ccgaaaatgc caatatcgac tgcatagaca atccgttcga
     2821 tcccgcgtgc ccatcggtcg tgaacaagac caccgttggc aatctcgtgt tgggagggct
     2881 ctatcacctc accgagcatc tgttgacgca cgttaataca acgtacgacg tccgtgagac
     2941 tcgtttctta aactttcgag ctgcggctaa acttctctcg cgttgcgagt gttggacttt
     3001 aaccttgggg ttgcagcgag acataaaccc agccaagact actttcaatt tcactttcgg
     3061 cttgcttggt ttgggcggat ctgagcagag ttcactcaga tgagcgtaaa gcctaaactg
     3121 ttccgaccgt tcgacagaac cgcgtttcct gttccttgat cctgaaaatc gatcgatcac
     3181 cgggttcgtg acagggccca gacgaacctg gatggcagcg cgcctcgcct cctttgcgta
     3241 gcttagctat ggttggcagc atgattgacc ttcaaaaagg ttcgtccagt ttagtgacgt
     3301 tctaaattgt tcgcacaatc ttttccgcag ataaaacttc ttcatcagtt ccaatctctt
     3361 gttggaatat ggtatgaaaa caagtcgaca tcgtcgttaa taatcaaagg actctgcaac
     3421 caacaatgcc aactcatgtc gtcgtaaccg gcgctgccgg ctatctcgga tctattttgt
     3481 gcgagcattt gctcgcggca ggatatcggg tgaccgcagc tgacgggctc atctatcgcc
     3541 aaagaacctt attccatctc tgcgccaatc ctttatttga tttcgttgtc ggagacgcgc
     3601 gcgatgagca gttgatgcgc cggctggtca aagatgcgga cgtgctgatt ccgcttgcgg
     3661 cgatcgttgg agccccggca tgcgatcgcg atccatggct cgctcgttcg gtcaatctcg
     3721 aagccataag actactcgcg cgtttgcgca gcaacgatca gcaaatcgtt tatcccacca
     3781 ccaacagcgg ttacggcacc aaatcgggag atgttttttg taccgaagat actccgctcg
     3841 agcccatatc gctttatggc caaacaaaaa cggaagccga gtcggagatc ctggaaagga
     3901 ccaatacggt caccttgcgg cttgcgacag tttttggcat gtcacctcgc atgcgtcttg
     3961 atctgctggt caaccacttt gtctatgccg ccgtcacaga ttgttatatt gttatttttg
     4021 aaaaggattt caagcgaaac tacatccata tacgcgatgt cgcggattgc tttatccact
     4081 gcataaaaaa cggcaagaaa atggccggcc ggccttacaa tgctggactc gatagcgcca
     4141 atctctccaa agaagcactg gctttgaaga tcaaagaata tgtgcctaat ttttacatcc
     4201 acttcggcca ggtcggaacc gatccggata agcgcaatta cattgtctcg aaccaacgcc
     4261 tgcgagatgc tggatttgaa gcgaagcgat ccttggactc aggtatccat gagttgctca
     4321 agggctatcg catgctgggc cgcgctgaat tcgccaatat ttagttattc acggccgaga
     4381 gaaatcattg tgggacagga ccgcagcgct ttttcagcac tcagtgtcgc aattctcgcg
     4441 ggtggactag gaacaagatt gcttccggtg gtttcagatc gacccaaagc gttggcggag
     4501 attcatgggc gcccttttct agcatacttg cttggccagt tagctgacgc aggatcctct
     4561 agagtcgtgc tatgtaccgg acatcttggg gaacaaatcg agcaagcatt cggcgaaaga
     4621 tatcgaactt tgcagatttt ttattcgcgg gaaaggcggc cattgggcac cggtggggcg
     4681 ctgcgattgg ctttgccgta tcttctgtct gatccggttc tggtcatgaa cggagactca
     4741 ttttgcgcca ctgatttaac gagcttctgg cattggcatg cgtctcgcgg ctctcgagcg
     4801 acgatgctat tggccgaggt gccaaacacg caacgttacg gctcggtgaa gatcaatcat
     4861 gatggcgcgg tcacccagtt tgccgaaaaa agacaaggcg gtccagggtt gatcaacgcc
     4921 ggggtgtatt tactgagccg ccgggttatc gattcgattc ccgaggacac gcctgtttca
     4981 ttggaacata accttttccc ggccttgatg aaccaagggc tttatggtta tcaagagcga
     5041 gggcgttttc tcgatatcgg cacaccagag gactttgctg ctgccgaaga attttttgcg
     5101 cccatcaacg aacgtgaaac gacggcggtt tgtagtactc gatagggacg gcacgatcat
     5161 cgaagaggtg ccttaccttt cggatccaag acaaattgcg ctgattcccg ggaccggcaa
     5221 ggcgctccgg gaacttagcg agatgggctt tgggctggtt gtcatcacca atcaatcggc
     5281 ggttggacgg agatattttg atgaagcgca gttggctcgg attcatgaca ggttgttaga
     5341 gatgttgcgt gctgaacaaa taactctcga tggtctttac ttctgcccgc atactcctca
     5401 agaccagtgt ttgtgccgta aaccgaacac cggattgatc gaaaaggcat cggaggattt
     5461 ggagtttgat ctcaggggga gcattgttat cggggacaag gcaagcgata tcgagatggg
     5521 ttgccgggtt ggagcgttga ccatgctggt acgcacgggc tatggggctc aagtcgccgc
     5581 cgagcagaag gttgagccgg actatatcgt cgatgatttg ccggcggccg tgcaagtaat
     5641 tgacacgatt tgtagaaagg tgcgtcatca atgatcatca gcaggacacc atttcgtatt
     5701 tcgttctttg gtggcggcac cgattatcct gcatggtacc gcgaccacgg tggcacggtg
     5761 ctgggagcga ccatcgataa atactgctac ctcacgtgtc gttatcttcc gccgtttttc
     5821 aaacatagaa ttcgggttgt ttattcacag attgaaaact gccaagcggt cgatgaaatt
     5881 gctcacccgg cggtgcggga agtattgcgt tacttgcaga ttgatcgagg ggtggagatt
     5941 caccacgatg gtgaccttcc ggcccgaagc ggcatgggct cgagctccgc cttcacagtg
     6001 ggtttgttgc acgccctgta cgcgcttaag ggacagatgc cgaccaagcg acagctggcg
     6061 gcggaaagca ttcagatcga acaggagatg atcaaggaaa ccgtcggctc gcaggatcag
     6121 gtcttggcgg cattcggcgg atttaatcat gtggtttttt cacaagacgg tgaaatcacc
     6181 gtcaagccca ttaccttatc aggcgatcgc ctgcgggagt taagctctca tttaatgctt
     6241 ttctataccg gcatagagcg cactgcctcg gatattgccg gcagttacgt tacggatgtc
     6301 gacggaaaaa gaagccagtt gcgcatcatg aaagggctcg tcgaagaaag cctcgcaatt
     6361 ttaaataatg gacaggatat aactgctttc ggcgctcttc tcgatgaggg atggaaagcg
     6421 aagcgcagcc tgagcgcgcg agtttcaaac agctacgtcg atgagctcta tgcccatgca
     6481 accgcaaacg gcgctctcgg gggaaagttg ctcggtgccg gtgggggagg ttttatgctg
     6541 ctgtttgcgc ctcccaacaa gcagaatcaa gtcaaagagg cgctgaaaag gtttatttcc
     6601 gttccgtttc attttgattt ttctgggagt cagatcatct tcttcgatca cgaaaaggac
     6661 tattcggccg aagaaaagat tcgcgattca caagagtatc attttgttgg cgagctgaat
     6721 gaccggttgg acagagattt gtagtcggcg ggcctcaccg gctcgccacc ctccaatcta
     6781 ttcgtttatc aatgcacaaa gacgcgagaa tctttattgc cgacgagcac ggactgatcg
     6841 gagaggttct caaaagacgg ttgagagacg acggttatca gaatcttctg ggcgactctg
     6901 ggaacccttc gttaatcgat gcccgggaag tcgaagcatt tttcttgcgg actctgcctg
     6961 aattcgtttt tgcggtgggc ggcaagtcgg gcggcattgg cgccaatcaa aaacttccgg
     7021 ccgatctgat gctgaacaac ctgcatgtgg attgccatgt catcgacagt gcgtatcgtt
     7081 gcggcgtcaa gaaattgctt tatatgggca gctcctgctg ctatccgcga gattgcttgc
     7141 aaccgatgca agtttcgtct ttgatgacgg gagttctgga atcgaccaac gaggcctatg
     7201 cgaccgctaa gcttgccgga atcaaattga ctcaagccta tcggcggcaa catggcgccg
     7261 acatgattag cgccattccc gccaatgtct ttggacccgg cgatgacttc ggcgtcgagg
     7321 actcgcatgt gatcgccgcc ttgatggcga agatgcatca ggccaaattt tcccaaacgc
     7381 aaagggtcga agtctgggga tcgggtttgc cgcgccggga atttgtttac gttactgaca
     7441 tcgctgatgc agccattttc ttgatgaagc attatagcag ccccgtgccg ataaatgtgg
     7501 gaagcggcgt agacgcgtca atcagggaga ttgccgagct gattaaagac gtagtcggct
     7561 tctcgggaga gctctgcttt gacccgaaga agccggatgg gatgccgttg aagtcgctgg
     7621 attcaagcga gcttttcgct cttggatggc agccaaaaat accattacgt ttagggcttg
     7681 agaaaaccta ccagtggtac ctggagactc gtcacacgaa tgcctaacgc catgaacgaa
     7741 cgaatcttta aatctcttta tcgcatccgc cgggtggagg aagagattgc tcggatttat
     7801 cccagcgaca agatcaagag cccggtgcat ctctcaatcg gtcaagagtg gatagccgtg
     7861 ggcgtgtgcg aggctttgcg gcctgaggac accgtattcg gcagttatcg cagtcatgcc
     7921 tactatttgg ccaaaggcgg tgatttaaaa aaaatgttcg cggagctttt cggcaaggct
     7981 accgggtgtg cgaagggaaa aggcgggtcg atgcaccttg ttgatgtcgg ccatggggtc
     8041 atgggagcgt cggcagtggt cggaacgact attcctaatg ctgtcggcta tgcctacgcc
     8101 atgaaactgc agaagagcaa aaatgtcgtc gtgagttttt ttggcgacgg agcgaccgag
     8161 gagggcgttt tttacgaaag cctcaatttc gccgcgctga aggagctccc ggtgattttt
     8221 gtctgcgaga ataatttcta cgcgatccat actcatatat cgcgtcgtca gaagaccccg
     8281 aacccgtgcg acaaggccaa agcccaaggc atcgtagccg agcagatcgg cgatggcgat
     8341 atgcggcggc tctatgaacg ggttggtgcc gcggcggatc tcatgcgcgc cggccggagt
     8401 gggccgtatt tctttgaatg tcttacctat cgctggaaag aacatgtcgg ccccaacgag
     8461 gatttcgatt tgggttaccg gagccggcaa gaagcgagcc catgggtcga acgcgacgca
     8521 ttgccgcgaa tcgaatgcct tctaactgcc gaagcttgta gccggctaaa aaccgaagtc
     8581 gaagaggaaa tcagggcggc catccgcttc gccgaggaaa gcccgtatcc ggaaccggcg
     8641 gaattattta tggatgtgat cggggattaa atggaacgta ctctgtccta tatcgatgca
     8701 attcgcgaag ccacggatca ggagatggcg cgggacggat ccgtggtgat atttgggctg
     8761 gacgtcgatg atcccaaggc aattcaagga acaacacgcg gactgctgga gaagtatggc
     8821 ccggaacgtg tcttcggaac tccactttcc gaggacgcca tgaccggagt cgccattgga
     8881 atggctttag cgggggtgcg gccgattcac gttcacatcc gcatggattt tatgttgctg
     8941 gcgatgaatc aactggtgaa catcgcggct aagagccgct atatgtacgg cggtcaagtt
     9001 catgtgccga tggttgttcg ctgcatgatc gggaagagtt ggggacaagg cgctcagcac
     9061 tctcaaggat tacaatcgct gttcatgcac atccccggtt tgaaagttgt ggcgccggcc
     9121 aatccctatg atgccaaggg atgcttgatc caggcgatac gagatggtga tccggtcatt
     9181 tatgttgagc atcgaattct tcatttccaa aaaggcctcg ttcctgaatc cgaatatggg
     9241 gttccagccg gcaaagcccg ggttctggca gaaggcgaag atattacact ggtcggagtt
     9301 tcgtacatgg tgttggagtg tctgagagcg cgcgattatc ttgcatcgac cggtatcgac
     9361 gccgaagtga tcgatcccgt ctggttgagc ccccttgaca tcgatacgat cgtaaaatct
     9421 gcgcgaaaaa ccggccggct gtgtgtcgtc gacaatggat ggacagcctg tggcgccggc
     9481 gccgaaatag tcagccaagt gatcgaaaga gtgcaacacg acaggcatgt agccgtgcag
     9541 cgcctgggtt atgcgccggt gacctgcccg ccgactccgg ttctcgagga tctctattat
     9601 ccaaacgcgc gcacgatcgc atcaagcgcc tatgatctcg tcaaaggagg caagaacgct
     9661 tggttgccgc aggaacgaaa agaccttgcc gagttcgaat ttcgcgggcc attttaatcg
     9721 aatcatgaat actatccaaa atctcgattg gccgttaatg aagaacaatc tggctaagga
     9781 ggatttcgcc aaagtcgttg agctgctgca tcgggaggac ccgatcttga cgcaatccga
     9841 tcaggtcaaa gagttcgaga aagaatggtc ggcttggttg ggtgttaaac acagcgtttt
     9901 tgtaaattcc ggttcgtcgg ccaatctcct aactatgtgc gcgcttaaag agcgctacgg
     9961 ctcgggagaa atcatcgtcc ctccattgac ttgggtttcc gatattgctt cggtgatcca
    10021 gtccgggttc aaacccgtat tcgtcgatat cgatcgccgt acgcttggaa tggaccatca
    10081 acagatcctt gaaaaaattt caccgaatac gagagcggta ttcatgacgc acgtgttagg
    10141 cttcaacgct ttgaaaaaat cgctcgttgc cgagcttgaa gggcggggta ttccgcttat
    10201 cgaagacgtc tgtgagtcgc atggggcgac ctgcgagggg cgaaagctgg gaaccttcgg
    10261 gcttatatcc aacttctcct tttactatgc gcatcatatg agcacgatcg agggcggtat
    10321 gatctgcacc aacgacgccg atctatatga aattcttcgg atgcttcgtt cgcatggtat
    10381 ggtacgggag tcgacttcgt ccgagctcaa ggaaaacttc tatcgcaact atccggacct
    10441 gaacccggac ttcatatttg cctttccggc gtataatgtt cgaagcacgg aaattaatgc
    10501 ggtgatcggc cgttctcaac tcaagcgtct cgatgccaac aacaaaatca gatcggagaa
    10561 tctaaccttg ttcctgtcac acctacatcc tgaaaagtac cagactgatt ttgccacggc
    10621 aggaagctgc aattatgcgt tcacgctagt gctcaaccga gctgatccgg tgttatgcga
    10681 aaacgtgatg caaatgatgc gtcgctgcaa cgtggaattc cgccgcggca cctcgggcgg
    10741 cggcaatcaa ttgaggcagc cttatctgag aagaatcatg ggtgagcgcg attttgaaaa
    10801 gtatccaaac gtcgatcacg ttcatttcta tggcttctac ataggaaact atccagacct
    10861 cgagcaagaa aagatcacgc agttgtgttc tctcttaaac agcgtctcgg gtgaggaagc
    10921 gcgttatgtc tcgtaatggc caattggatc tggtactggt tcatcccgga acccggatgc
    10981 aggtttatca atctttggga tcgaagctgg cagccatcga gcctccggta tgggctagct
    11041 tattggcaac gtttgttcgt aaacacgggt tctcggttca aatcctagat gcggaagcgg
    11101 acaatctaac accccaagag actgcaactc ggatcgagga gatggatccg cgattggcgg
    11161 tggtcgttgt ctacgggcat caaccgtcgg cttcgaccca aaatatgacg gtctccggct
    11221 tgatctgcgc ggcgctcaaa gaactcaacg gcgatcgtaa attggcgctg gtagggggac
    11281 atgtggcggc gcttccggaa cgcaccttgc gggaggaagc ggccgatttc gtgtgcaccg
    11341 gagaaggccc ctataccgtc ctcgatctgt tggaagcggg gaaagcgaca tcaccggatt
    11401 attcgaaggt gcggggaatc tgctattgga aagacggtgt tgcaatgtca accccggcgg
    11461 ctccgctggt caaaaattta gacgaagaaa tacccgaact cgcatgggat cttttgccca
    11521 tggagaaata tcgagcgcac aattggcact gcttcggaga tcttcaacgg gagccttatg
    11581 cctcgttcta cacaaccttc ggctgtcctt ttcattgcag tttctgctgt attcaagcgc
    11641 catttaagac cggagagaag gaagcgggtt ggaaggaaag cgtcaatagt tatcgtttct
    11701 ggagcgcgca agcggtgatc gcccagatcg ataaacttgt caaccagtat ggtatccgga
    11761 acattaagat agccgacgag atgttcgtcc tgaattcacg gcacatactc gggatttgcg
    11821 atgcgattat cgaacgcggc tacgatttga acatctgggc ttacgctcgc gtcgacacgg
    11881 tcaaggacgg aatgcttgaa aagctcaaac gcgcaggttt caattggttg gcgtttggca
    11941 tcgaggccgc tagtgatcgg gtgcgcgatg acgtggataa aggattcgac caagaagaaa
    12001 tcttccgcac gctgggaaag gttagagcaa cagacgttaa cgtgatcgcc aactatattt
    12061 ttggtcttcc ggaagatgat ctggaaagca tgcaatcgac gctaaatctc gctctcgaac
    12121 tcaactgtga attcgccaac ttctattctg cgatggctta ccccggatcc cagttataca
    12181 accttgcagt caaggaaggt tggccgcttc cggagaagtg gtccggctat tcgcagcatt
    12241 ccgtcgacac cttgcccttg ccgacgaaac atctatcggg cgcggaggtg ctgcgatttc
    12301 gcgatcaggc atttcaacga tattttagca gcccacagta tttgcggatg atggaggcca
    12361 agtacggtcc ggccacggct gagcatatcc gtgaaatggc cgcgcaacca ttggtcagaa
    12421 aaatcttggc tgccgcgtga gcgccgacct ccgtgcgacg ccattctgtg cgaaagcgaa
    12481 gatctgccga ctcgaccggc gcgaaatctc agcacccgga acgagaagca cgaacgcttg
    12541 cctgcgagaa cagccagttt catattttct ttgacgagtt gagtctcaaa gggcgcacga
    12601 cgattacgga ttatctggtc gttgcgccca agcgagtcgc cgcagacctc gtcaccggag
    12661 tggcgattct accggtgttt gaaggaaaga tcggcttgct ttggatttac cggcatccca
    12721 tcgggcgata cgcgtgggag atcccgcgtg gcttcatcga tcagcaggag gtctctgcga
    12781 cagcggcgag tcgagaactg aaggaagaga ccgggcttga ttgcaagccc gaagcgattc
    12841 gatcgctggg ctttatcact cccgatgccg gaattttggc ggcccgagta cagctctttg
    12901 ccgcgaccca atgtgaaaag gagcgggatt ttgcacccgc ggagatcggt catcgagaac
    12961 tccggttctt caacatggaa caggttgaaa aaatgattct tgtttccgag ttgcaggatc
    13021 catcaaccat cattgcattt ttcctgtaca gtcgatttga taatcaggtc ccggatggtt
    13081 cagtgtgaaa gtgactttgc tggttctagc actgaatgaa atcgagggca tgaaggcgat
    13141 catgccgcgg gtgaaaaaag aatggtacga ccagatcatc attttggacg gcggatcgac
    13201 ggacggcacc atcgagtacg caaaaaaaca gggatacttc gtctacgttc agaaaagacg
    13261 cggatttcgg cacgcctata ccgaagtatt gccgtacatt gagggagacg taatcgtgac
    13321 cttcagcccc gacggcaact cggttccgga gcgcattccc gcattgatcg agaaaatgaa
    13381 cgaaggatac gacatggtga ttggctcccg ttacctggac ggggccaaga gcgatgatga
    13441 tgacgtgatt acggcttttg gcaactggct gtttacgaag acgatcaacg tcctgcatgg
    13501 gggatgctat accgactcga tggtgatctt ccgcgcatat aagactcagc tgatctacga
    13561 tctcgagctc cataccgatg aagcctactc cgccgctgag aagcttttcc atacgacgat
    13621 cagttgggag cctctgctgt cagtgagggc ggctaaacga agactcaagg tcggcgaaat
    13681 accggccgac gagccgccac ggataggcgg ccaaagaaag cttcgagtca tacaatgggg
    13741 tggagcgtac tattttcaat tcctgcggga gaaattgttt tggcgttaaa tttagccgta
    13801 tttagcggaa aatttgagct cgtagttagt ttagtagacc ttgggtaacg atgacacgcg
    13861 ggatgctatt gataccttcc acaggaaatc agtcgttttt atttttatac gttggttcgt
    13921 gccgcaccat ttagcattca tccagagtgg caacactacc gtttgatcag ctagct
//
LOCUS       DASUVS010000143         8002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1802374, whole genome shotgun sequence.
ACCESSION   DASUVS010000143 DASUVS010000000
VERSION     DASUVS010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8002
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802374"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VF182_08100"
     CDS             <1..492
                     /locus_tag="VF182_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437079.1"
                     /translation="RLLAMKAGQLEAAIVLPPITTIAQREGMNVLLDMSELGAEYQIT
                     GLASSQTFITKNRPAAMKLMRSFVEGIHFYKTRKKESMAIIAKYMRTNDMEAVGATYD
                     YFANKIVPKKPYPTTQGIKALLELAAKERPDAAKVPPERIMNTSLLKELDDSGFIDRL
                     YQN"
     gene            629..2320
                     /locus_tag="VF182_08105"
     CDS             629..2320
                     /locus_tag="VF182_08105"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6437080.1"
                     /translation="MAENGKQKTVAQAYLEVAVNHGVDYVFGLPGTSGQEFIGTIADQ
                     EKVRFVLALHETCVVSMADGHARVTGRPSLAQVSTLPGSVNAVGALYDAYRDRSPVVV
                     TSTHVDTRIVGRDSHTEGKDLVELTKQFTKWSAEVHRSDRIPEFLNRAFKVAATPPTG
                     PVYLSLPSNLLGENIDQPNPVAARSRITPRIAADPEALQQAAQMLADAKRPLILAGSG
                     IAKSGGTSELIKFAEMLGAPVVMEPRYSFLSFPTDHPQSFQIAERNPSFNLPVWGDPD
                     VILAIGCRLIREYRYLPEPVMKPDTRCIHIEEDPWEIGKVFPVDLGIIADGRSALRSL
                     LAVYPTVARGANGSHERLEVIRKAQEQVKAELESRAKQGWDGTPISAARLARTMDRLL
                     EKDTLIVNESPTSKDILMANFKFSTGRSYFSNSSGGYLGWGLGAAIGAKLAAPKRRVV
                     ACLGDGSSMFGLQGLWTLAKYRVPLIVIVFNNRAYMAVKNQFRGSEERIRLAAEMGAE
                     LVGPELNFARLADTFGIFGQRVEQPEAVEAAIKRALEQNGPALIDVVIGQNTRKD"
     gene            2456..2830
                     /locus_tag="VF182_08110"
     CDS             2456..2830
                     /locus_tag="VF182_08110"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6437081.1"
                     /translation="MAEDPKEAFKEVGLGSPNNAYDIIAKLDPEYFEKLKGLYVDGTF
                     GRDGALPRKTKELIMIGICCALLRPRGIRLHSERALTLGATPKELMEAMEVAAIPGGM
                     PGLWAGAEALQEILDAKGIEFE"
     gene            2827..4029
                     /locus_tag="VF182_08115"
     CDS             2827..4029
                     /locus_tag="VF182_08115"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6437082.1"
                     /translation="MTEIRPGPLSGVRVLDLTRVVAGPYCSMFLGDLGAEVVKVEQPG
                     AGDDTRGWGPPFAGGESAYYLCINRNKQSLTLDLKSQRAVELLRQLVKAADVILENFR
                     PGTMERLGLGEKELREINPRLIYASLTGFGADGPMSDWPGYDLIVQAWGGLMSITGTP
                     DGEPVKVGVAIIDLVAGLMLGKAITAALFAREKIGVGQRIDTSLLEAEVASLINVGSN
                     YLVGGKVPSRWGNAHPNIVPYQNFQTADGYLVIGVASEVIWQRFCEAIGQTDLIEDSR
                     FADNSKRVENRSELIALLSQIFLQRRNDAWFKLLTDAEVPCSPVQTVDQVFQAPQVLQ
                     RDMLIEVDHPTAGKVRMAGIPVKFSVTPATVRLPPPLLGEHNETILKSWLGLSADSIG
                     ELKKEKVI"
     gene            4026..4487
                     /locus_tag="VF182_08120"
     CDS             4026..4487
                     /locus_tag="VF182_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX6437083.1"
                     /translation="MTGKTFEEFHAGDHYNTGRRTITEHDILQFVTLVGLTEPLFLDM
                     EYIRNESLYGDRIAPGSLTFGMAEGLTVQTGLIHGTGMAFAGLDKMRLFGPVKVNDTI
                     EVEIEVLDTKPVPARGGGIVRYRQWVKNQRGETIMEYDVARLIRGVAPETK"
     gene            4536..5501
                     /locus_tag="VF182_08125"
     CDS             4536..5501
                     /locus_tag="VF182_08125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6437084.1"
                     /translation="MNRSEPKFGILLPTREVILSGRAEPSSIYDIADRTEALGFHSVW
                     VGDSVVAKPRLDALTTLAAVGARTRRIRLGTAVYLAALRNPVLVAHMVGTVDWLSHGR
                     VDLGIGYSRPNDPVQEHEYKVLGHDPAKRIKMSEESVQIMRKLWRENDVSYNGTFSSF
                     ERVTVEPKPVQSGGVPIWLASNNIEPGLKRVARMADGWLNNITDPKIYQQCLEKIRAY
                     ALEAGRDPQVIEPGLYLTLAAGDNNVIREGQNFLSQYYNRPYEATAKAMLCVMGSWEQ
                     VMDQIQAYAEAGARSLVLRFAASDQIGHLEACAEQLSKRGYLGQS"
     gene            7038..7331
                     /gene="spoVG"
                     /locus_tag="VF182_08130"
     CDS             7038..7331
                     /gene="spoVG"
                     /locus_tag="VF182_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HEX6437085.1"
                     /translation="MEVTEVKIFPVDEDKLRAYVTITLDHCFVIRDLKVIKGTTGYFV
                     SMPSKKRKDGTYKDIAHPINSETRRMLEEKVIHEYEQTIQNSGIGDSPHVSTT"
     gene            7675..7959
                     /locus_tag="VF182_08135"
     CDS             7675..7959
                     /locus_tag="VF182_08135"
                     /inference="COORDINATES: protein motif:HMM:NF015959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SpoVG family protein"
                     /protein_id="HEX6437086.1"
                     /translation="MQITDVKIYRRVQGTLRGYADVTFDDCLSIREFKLLRTATGYRV
                     CMPEVKKQDGKVRRVAYPTDDKMLKAIEDTVIAEYKKVTRWTVAPGVLRY"
BASE COUNT         1890 a         2171 c         2244 g         1697 t
ORIGIN      
        1 cgcttattgg caatgaaagc tggacaactc gaagccgcca tcgtgctgcc gccgattacg
       61 accatcgccc aacgcgaggg catgaacgtg cttttggata tgtccgagct cggcgcggaa
      121 tatcagatca ccggtctggc cagcagccag accttcatta cgaagaatcg gcccgccgcc
      181 atgaagctga tgcgctcctt tgtcgaagga atccatttct acaagacccg caagaaggag
      241 agcatggcga ttatcgctaa atacatgcgc accaatgaca tggaagcggt gggcgccact
      301 tatgattact tcgccaacaa gatcgttccc aagaagcctt atccaacgac ccagggaatc
      361 aaggctttgc tcgagctcgc ggcgaaagag cgtcccgatg cggcaaaagt accgccagaa
      421 cgcattatga acacgtcgtt attgaaggaa ctagatgaca gcgggtttat cgatcggctt
      481 tatcaaaact gagcgtgtta aaggttgaca gtttattcat tggacctcgt cggagaactg
      541 gaaaactgtc aaccagcatg cggtttggac ctggaagaca tgaactctta cggaaacgct
      601 gaaccgcagt cgcggaggag aaaagaatat ggctgaaaat ggtaagcaga aaaccgtggc
      661 gcaggcctat ctcgaggtcg cggtgaacca cggcgtcgat tacgtttttg gtctgccggg
      721 aacgagcggc caggagttca tcggcactat cgccgatcag gaaaaggtcc gattcgtcct
      781 cgctctgcac gagacttgcg tggtgtcgat ggccgatggc cacgcccggg tcaccggccg
      841 gccttcactg gcacaggtaa gcaccctgcc cggttcggtc aacgccgtcg gcgctctcta
      901 cgacgcttac cgcgaccgct ctccggtggt ggtgacctcg acccatgtcg atacgcggat
      961 cgtcgggcgc gattctcaca ccgaaggcaa agaccttgtc gaattgacca aacaattcac
     1021 caagtggagc gccgaagtgc atcgctccga tcgtattccc gagtttctca atcgggcatt
     1081 taaagtcgcg gcaaccccgc ccaccggccc ggtctatcta tcgcttccca gcaatcttct
     1141 cggcgaaaat atcgatcagc caaatcccgt cgcggcacgc tcgcgcatca cgccgcggat
     1201 tgccgccgat cccgaggcct tacaacaagc ggcgcaaatg cttgcggacg cgaaacggcc
     1261 cctgatcctc gccggcagcg gcattgccaa gtcaggcggc acctcggaat tgatcaagtt
     1321 cgccgaaatg cttggcgcgc cggtcgtcat ggagcctcgt tattcgttct tgagtttccc
     1381 caccgatcat ccgcagagct ttcaaatcgc cgaacgcaat ccgtctttca atctgcccgt
     1441 gtggggtgat ccggacgtga ttctcgcgat aggctgtcgt ttgattcgcg agtaccgtta
     1501 tttgcctgaa ccggtgatga agcccgacac tcgctgcatt catatcgagg aagatccttg
     1561 ggaaatcggc aaagtttttc cggtggatct tggcatcatc gccgacggga ggagtgcgct
     1621 gcgctctctt cttgcggttt atccgacagt ggctagagga gcgaacggga gccacgaacg
     1681 cctggaagtg attcgcaaag cccaggaaca ggtaaaagcc gagctcgaat ctcgcgcgaa
     1741 gcagggctgg gacggcacgc caatcagcgc agcccggctt gctcgaacca tggaccggct
     1801 gctcgaaaaa gatacgttga tcgtcaacga aagcccgacg agcaaagata ttctcatggc
     1861 gaattttaaa ttttcaacgg gtcgctcgta tttttccaat tcctcgggcg gctatctcgg
     1921 ctggggcctg ggcgcggcca ttggcgccaa gcttgccgcg ccaaagcgcc gcgtcgtcgc
     1981 ctgtctgggc gacggcagca gcatgttcgg gctccaggga ctctggacgt tggcgaaata
     2041 ccgcgtgccg ctgatcgtca tcgtattcaa caaccgcgcc tatatggcag tgaaaaatca
     2101 gttccgcggc tcggaagaaa ggattcgtct ggccgccgag atgggggcgg agctggtcgg
     2161 acccgagttg aacttcgcgc ggctcgccga cacattcggt atcttcgggc aaagagtcga
     2221 gcagccggag gccgtcgaag ccgcgatcaa gcgggcactg gagcaaaacg ggccggcgct
     2281 gatcgacgtt gtcatcggtc agaacacacg caaggactga ggattccgtg aaccacgaag
     2341 gtcacgaaga gcacgaaggt tgcatctgtt ttcctaatcg tcgtggcctt ccatctcctt
     2401 cgtggtggtc aggcctcgtc ctacacgaac gagcgaacca agagggggac gaaatatggc
     2461 agaagacccg aaagaagcct tcaaagaagt aggtctaggc tctccgaaca atgcctacga
     2521 catcattgcg aaactggatc ctgaatactt tgagaagctc aagggactct acgtcgacgg
     2581 caccttcggt cgcgacggcg cattgccgag aaagaccaag gagctgatca tgatcggcat
     2641 ctgttgtgcg ttgctccgcc cgcgcgggat ccgcttgcat agcgaacgag cgctcaccct
     2701 cggcgcgacg ccgaaggaac tgatggaagc gatggaagtc gcggccattc ctggagggat
     2761 gcccgggctt tgggccggtg ccgaggcgct ccaagaaatt ctcgacgcaa aaggcatcga
     2821 gttcgaatga ccgaaattcg gccggggccg ctaagcggag tgcgcgttct cgatttgacg
     2881 agggtcgtcg ctgggccgta ctgctcaatg tttcttggtg atctcggcgc tgaagtcgtc
     2941 aaggtcgagc aaccgggagc gggtgacgat acgcgcggct ggggaccgcc gttcgcagga
     3001 ggcgagagcg cctactacct ctgtatcaac cgcaacaagc aaagcctgac actcgatcta
     3061 aaatcgcaac gggcagtcga gttgctgcgc cagttagtca aagccgccga cgtcatcctc
     3121 gagaatttcc gacccggaac catggaacgc ctcggcttgg gcgagaagga attacgcgag
     3181 atcaatccgc ggctcatcta tgcctccctc accggctttg gcgccgatgg accgatgagc
     3241 gactggccag gctacgattt gatcgtgcaa gcctggggcg gtctgatgag catcaccggc
     3301 acgccggacg gggagccggt caaagtcggc gtggcaatca tcgatctagt cgctggattg
     3361 atgctcggca aagcaatcac ggcggcgctg ttcgctcgtg agaaaatcgg cgtcggacag
     3421 cgcatcgaca cttctttgct cgaggccgaa gtggcctccc tgattaacgt tggcagcaat
     3481 tatcttgtcg gcggcaaggt gcctagccgt tggggtaacg ctcatccgaa catcgtgccg
     3541 tatcaaaact ttcagaccgc ggatggctat ctggtgatcg gcgttgccag cgaagtgatc
     3601 tggcagcgtt tctgtgaggc catcgggcaa acggacttaa ttgaagattc gcgctttgcc
     3661 gataattcaa agcgtgtgga aaatcgcagc gaactgattg cactgctttc gcagatattt
     3721 ctccagcgcc ggaatgatgc gtggttcaag ttgctcacgg atgcggaagt tccctgctcg
     3781 cccgtgcaaa ccgtcgacca ggtcttccaa gcgccgcagg ttttgcagcg tgacatgctc
     3841 atcgaagtcg accatccgac ggcgggcaag gttcgcatgg cggggatccc ggtaaaattc
     3901 tctgtgaccc ccgccaccgt tcgcctgccg cctcctctgc ttggcgagca taacgaaacg
     3961 attcttaaaa gctggctcgg tttgagcgcg gattcgattg gcgaactcaa aaaagagaag
     4021 gtcatatgac aggcaaaaca tttgaagagt ttcacgccgg tgaccattac aacaccggcc
     4081 ggcgcacgat caccgagcat gatatcctgc agttcgtcac gttggtgggc ctgactgaac
     4141 cactgttcct cgatatggag tacatccgca atgaaagtct ttacggtgat cgcatcgcgc
     4201 ccgggagtct gaccttcggc atggccgaag gtctgacagt gcagacggga ctcattcacg
     4261 gcacaggtat ggcctttgcc gggctcgaca agatgcgcct tttcggtccg gtgaaggtga
     4321 acgacaccat cgaagttgag atcgaagttt tagacacgaa gccggtgccg gcccgcggcg
     4381 gcggcatcgt tcgttatcgc cagtgggtaa agaaccagcg cggcgaaacc atcatggagt
     4441 acgatgtcgc gcgcctgatt cgcggcgttg cacccgaaac aaagtgagtt caattgtcta
     4501 cttcatcaca agctcgattc gataaggctt cactgatgaa ccggtctgag ccgaaattcg
     4561 gcattctatt acctactcgg gaagtgatct tatccggcag ggctgaaccg agcagtatct
     4621 acgacatcgc cgatcgaacc gaggcgctcg gctttcattc cgtttgggtc ggcgacagtg
     4681 tcgtcgcaaa accgcggctg gatgcgctca ccaccctagc ggcggttggc gctcgcaccc
     4741 ggcgaattcg tctgggcacg gcggtgtatc tggccgctct caggaatccg gttctggtgg
     4801 ctcacatggt tgggactgtc gattggctca gccatggccg ggtcgatctc ggaattggct
     4861 acagccgccc caacgatccg gtgcaggagc acgaatacaa agttctcggc cacgatccgg
     4921 ccaaacgaat aaaaatgagt gaagagtcgg tgcagatcat gcgaaagctg tggcgcgaga
     4981 atgatgtttc ctacaacggc accttctcga gcttcgagcg tgtgacggtg gagcccaaac
     5041 cagtccagtc tggcggagtg ccaatttggc tggcgtcgaa taacatcgag cctggactga
     5101 agcgtgtcgc acggatggcc gacggttggc tcaataacat caccgatcca aaaatctacc
     5161 aacaatgcct agagaagatt cgggcttacg cactcgaggc aggtagagat ccgcaagtga
     5221 tcgagccggg actttacctc acgcttgctg caggggataa caacgtcatc cgcgagggtc
     5281 agaactttct gtcgcagtac tacaaccgcc cctacgaagc caccgccaag gctatgcttt
     5341 gcgtgatggg cagttgggaa caagtcatgg atcagattca ggcttacgcc gaagctggcg
     5401 cgcgctcgct ggttttgcgc tttgctgcca gtgatcaaat cggtcatctc gaagcttgcg
     5461 ccgagcagct atcaaaacgc gggtacctgg gtcaatcgta ggaacatgga aatgggcgct
     5521 gatacgacgt tcgagcgacg ctcgcacgat cgcgcatccg atttcggatt tccacatggg
     5581 cagacttaaa atacttatgt gagtccaagc tgtgatctac gacggcttac cgaaaagaaa
     5641 aatcgagagc agagggaaac tcagtaatac agggcatgaa tagaaacgcg gaggaataga
     5701 gggaaagaaa acacgacagc attacgaaaa atctagaaga gtgtaattcc gggcaaacca
     5761 ctgtcttggc ggcaaatccc ggaagcttct cgtaaaaact tggcaggaat gcagttcgat
     5821 tcgtttttgt ccgtttatgt tctgcttgcg gcagtttcgt tcagtcctga agcgtaatgt
     5881 ctgctggtgc ggcgctttgg attccatttc attggagatt ttaaaacctg accgggtggc
     5941 ttattaattg ctccgcccag ggtgcttttc aaaagaggga tcaagaatgc gaacatttgc
     6001 tgacaccgag atacaggcca gacttatacg tgagatgcgg gagctgtatg agaaacgggg
     6061 aaagctagaa gccggcattc gggcggtgga tacagccaga agcctccgca tcgaaaagga
     6121 cgccttgatt gacattgcgg cctatcgagc cttgctttcg cgtccacaga gcatgtgagt
     6181 cctgaaatcc tgtctgcgcg cgctgatatg aagtccttgc tgacccaccg tatcaaccgg
     6241 atagccgaaa ttgtccgcga cctcgatagg cgctctccgt ccggagttga cccggctatt
     6301 caaaccctct aacttttttc acacgcagaa agcgctcggc ccgttttcac ctcgcgccgg
     6361 tgtccataca acgacgagtt aggagacgaa catgcagacg tcggaggaca gacgcacaac
     6421 tgaagcaaaa cgtttggacg aattagccct ccaatatcgt acaagccggg atccagaagt
     6481 gctggccgag atgcgagcat tggcccgacg cctttccgaa acaaatcgat aatcccaacg
     6541 caggtgtcgg cgccggtggt cgctgtcgct tcttccctag tcccattcca tccccttact
     6601 agtccatcaa ctcgcgcata cttcgactta tcgcattcgc gagacaagcc gttggccccg
     6661 taatcctaaa agtgcaacta attcgccttg gagactaagc gcgtgggctg caacggtttg
     6721 gctcgatcat tgcggaggtg ccgctttgta gtactaatcc ggcagtaagg gcgcaaaacg
     6781 gattgtctgg tcgctgcctg cagtagctaa acgtcttgcg aactacagcg ccttcgacgc
     6841 tcttgagcga ctcgacgaac tcttgtgtca atataaggtc aacgggcttc gacttgcaca
     6901 tgggattgtc aatttggatt tgctctaatg cgacatctgc ataccggcag cactgaaaca
     6961 ctcggtacga tcgattcctc agattccgat acctgtagcc ttcactctaa gaacaacgcc
     7021 tcaaggaaga gatttaaatg gaagttaccg aggtaaaaat ctttcctgtg gatgaagata
     7081 agcttcgggc ttacgtaact attactctcg atcactgctt tgttatcagg gatctaaagg
     7141 tcatcaaagg aaccacgggt tatttcgtat cgatgcccag taagaaacga aaagatggca
     7201 cctataaaga tattgcccat ccgatcaata gcgaaacgcg tcgcatgttg gaggaaaagg
     7261 taatccacga atacgagcaa acaatccaga actccggaat cggagactct ccccatgttt
     7321 ccacgacgta aactgcggtc gacatgcctg gatggtttac caagacggcg acgcagaaaa
     7381 gtaagcgtct gatcaacagc attggtttat cttcgtcgac aattctacta ttcgcttcat
     7441 gagcgattgc ggcagtagct attccgcttc cacccgaatt atcgccaccc ggcttctcgt
     7501 caaaaccgtt atgcgtctga aatgcgtagt tctggaagcg gttttgacgc agtggcggac
     7561 caacctgggg ctgctttcct ctgtaagccc attcgggtgc gggcagcgat gcgcgctgtc
     7621 ttgactcgta ggcttgccca acgtataagc tccgccacca catcgggagc gaacatgcaa
     7681 ataacagatg ttaagattta ccgtagggtc caagggaccc tgcgcggcta tgcagacgtg
     7741 accttcgacg actgtttgtc gatcagagaa tttaaacttc tgcgaacagc taccggctac
     7801 cgagtttgca tgccagaggt gaaaaagcaa gacgggaagg ttcggagagt cgcctacccc
     7861 actgatgaca aaatgctgaa ggcgattgag gacacagtga tcgccgagta caaaaaagtc
     7921 accaggtgga cggttgctcc tggtgttcta agatactgat ctactgaatt ctcccgactt
     7981 gctcttcgac aaagtcgatc ac
//
LOCUS       DASUVS010000144         6670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_927655, whole genome shotgun sequence.
ACCESSION   DASUVS010000144 DASUVS010000000
VERSION     DASUVS010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927655"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VF182_08140"
     CDS             complement(<1..704)
                     /locus_tag="VF182_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437087.1"
                     /translation="MKRETISTNLIMIFFFSAILLLICFYRNAFGQEPTTKIRISNSA
                     LSVTALPLVAAREWNFFREQGLQAEIIMMNPAISNPAIAAGEIDYVAGVGPGSVAATL
                     AGLPLRAVWFSSNRISYFVTTSPQHKSLQDLKGKKIGITGGIGGTNHVALVIALEKLG
                     FNPKDFIILTTPTAELLRSLESGFVDAASLNPPTVFFAQRKGFHRVLDIGSLVEMPGG
                     GLTTLIKTITSKPDEV"
     gene            complement(887..2596)
                     /locus_tag="VF182_08145"
     CDS             complement(887..2596)
                     /locus_tag="VF182_08145"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX6437088.1"
                     /translation="MKAKAELTAPRPPVDPITLEVIRHGIVSITDQIDANIARTAFSP
                     YIYEYKDFAVGLVSADGELIAQCTGGMPVFVADSVGMAVRDGLAVYGRERLQRGDVIL
                     CNHAAIQGQHLNNTVMYTPIYAGAEMGSLVGFFAINVHWIDIGGVTPRSSDIFMEGLQ
                     LRSIKLWSKGEPMQEVYRIIENNTRFPIELLGDIAAQHAGCILGRNLTQSLAEKYGVE
                     TFLAALKAMLDQSEAAARERIRRIPDGRYGYETFFDNDGETDDALPIRVKVIIEGDEM
                     TVDYSGIAGQVRGCINSGYYGGGRTTARVAFKYLIANEEPANEGTFRPLKLILPEGKI
                     LSAHPTAPMGNYSQPFPTVIDAIIKALEHALPERVTGAHFGTFSGVRFRGKRDNGTLF
                     DCHDSGHGGWGACATHDGAGPFRTMAHGDTRIIPVELQESMYPYRIVEFGLRENSGGA
                     GTFRGGLGYRKRYEILGHCDLQAMFDRVKYPPWGVHGGKAGQSGQITVIKKSGDQEIL
                     HKSKAYPLEPGDSIIVETGGGGGYGPPHERKRASLERDLRRGYVSVEAARRDYGFAIE
                     RTR"
     gene            complement(2684..4738)
                     /locus_tag="VF182_08150"
     CDS             complement(2684..4738)
                     /locus_tag="VF182_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008075337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX6437089.1"
                     /translation="MSYAIAVDIGGTFTDLVAFDHQTQKVTYAKSPTNYDNLVEGILA
                     CFNKASIRPAEASLVNHGTTLVINSLIQRKGAKTALVTSKGFRDILEIARGNRPDPFD
                     LHYQRDEPLIPRELRLEVPERMSSKGEVITPLDLKALEKLAGEIRMLDIESLAIFFMN
                     SYINPAHEEAAAEKLKELLPDTYVTFSTDLTREWYEYERCSTVAANAYVGPQVSSYIG
                     QLDQDLKDQGFDGALFMMGSNGGLLSAERTCRQPIGLVESGPIGGCIGASAYAEKLGF
                     NNVVAFDMGGTTAKCALVENGRFSVNSVYYANGYVKGFPIKSAVIDIVEVGSGGGSIA
                     WIDSQNRLHVGPQSAGSTPGPVCYGRGGTEPTVTDANLILGRLNAGNFLGGEMALDMK
                     AAEAEIEKRIAKPLGYAGQSGAIQMADGIISIATVIMAGAIRKVSVEHGLDPRDFILF
                     SYGGGGPLHACALAHELYIPAVVIPPEPGNFSAIGMLLADARIDTSKTFVGILDEKTI
                     TTMAEVFAALEKDAAVALAAEFSSSETFFEHHAEMRFRGQRHNIKVPVSGLKDVQEIR
                     AAFERDYKRRYGHADAKAPAEFQALHLSAFAKLKQPQIARLPRAPIKAQPVQKRRVYI
                     GSAGGWTDAQIYNRDALEPGVSGAGPAVIEEYGSTTLVWPGDSFEIGELHEIRIKIKA
                     ES"
     gene            complement(4934..>6670)
                     /locus_tag="VF182_08155"
     CDS             complement(4934..>6670)
                     /locus_tag="VF182_08155"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX6437090.1"
                     /translation="AIARPTIDPITLEVIRHGIISICDQIDANMTRTAFSPYIYEYKD
                     YAVGFVGAGGELLAQSTGGMPVFVADSVGMAVKDGLAIYGRDNLQRGDVIVCNHAAVQ
                     GQHLNNTVMYTPVFAGAHRDELIGFFAVNCHWIDIGGSAIGSVSYSSTDIFMEGLQLR
                     SIKIWSNGEPMEEVYRIIENNTRFPLELLGDIEAQLGGCLLGRDLTQTMADKVGAGVF
                     LHALDVILDQCEAATRSKIRAIPDGVYHHEAFLDNDGVRDEKIPIKVKIIVAGDELTI
                     DYSEMSPQVRGCINSGYYGGGRTTARVAFKYLIATDEPANEGTFRPLKMILPEGKLIS
                     AEPTAAMGLYSIPFPTIIDCIIKALEPALPERVTGAHFGTFSSLSFSGKRTDTGAVFK
                     ANDSGHGGWGACATHDGAGPFRTMAHGDTRLIPIELQESMYPYRVEEFCLRQDSGGPG
                     KWRGGLGFDKQYILLAPCELWANFDRIGCPPWGVQGAKAAKSGQVLIFKNGNEEPELL
                     YKTENCPLQAGDRVRMSTGGGGGYGDPRERPIERVKQDVIRGFVSRQSARDNYGVAID
                     AEGRAARTKPER"
BASE COUNT         1398 a         1925 c         1885 g         1462 t
ORIGIN      
        1 ctgacttcat ccggtttgct cgtaatcgtt ttgatcaagg tcgttaaacc gccgccgggc
       61 atctctacta acgagccgat atccagcacc cggtgaaacc cttttcgctg cgcaaaaaat
      121 actgttggtg gattgagcga ggccgcatcg acaaagcccg actccaaaga ccggagtagc
      181 tcggccgtgg gcgtggtcag aatgatgaaa tccttgggat tgaagccgag cttctcgagg
      241 gcgataacta acgcaacgtg attggtcccg cctatgccgc cggtgatgcc gatttttttt
      301 ccttttagat cttgaagaga tttgtgttgc ggcgaagtcg ttacaaaata ggatatccga
      361 ttggaagaaa accacaccgc tcgcagcggc agtcccgcca aagtagctgc cacggaacca
      421 ggcccaacgc cggcgacata gtcgatctcg ccggccgcaa ttgccgggtt cgaaatcgcg
      481 gggttcatca tgatgatctc ggcctgcagt ccctgttcac gaaagaaatt ccattcgcgc
      541 gcggcgacca gcggcaaagc ggttacgctc aatgcggagt tggaaatgcg tatcttcgtt
      601 gtcggctcct ggccgaacgc gtttcggtaa aaacaaatca gcaataaaat tgcgctgaag
      661 aagaaaatca taatcaagtt agttgatatt gtctctcgct tcatcgcgaa tccttcattc
      721 actgctcagc caagtattct gtggacgtcg gtgcccaagg taagatcgaa gagatccaac
      781 cgaggaagtt cacgtgttat ccgatattat cggggcactg gcgagctgaa gctgttctat
      841 cggcccggtg ttccattcct gattggcggc agcgggtcga tcggaactag cgagtccgct
      901 cgatggcgaa tccgtaatcg cgccgagcgg cttctacgga tacatatccg cgccgcagat
      961 cgcgttcaag cgatgcgcgc ttgcgttcat ggggcgggcc gtagccgccg ccaccgccgg
     1021 tctcaacgat gatcgaatcg ccaggttcca atggataagc cttactcttg tggaggattt
     1081 cctgatcgcc ggattttttg attacggtga tttgtccgga ttggcctgct ttgccgccgt
     1141 gcactcccca cggcggatac ttgacccgat cgaacatcgc ttggaggtcg cagtggccca
     1201 ggatttcgta tcgcttgcga tagcccaggc cgccgcgaaa tgtgccggcg cctccggaat
     1261 tttcacgcaa gccgaactcg acgatacgat agggatacat cgactcctga agttccacgg
     1321 ggatgatgcg cgtgtcgccg tgcgccatcg tccgaaacgg cccggcgccg tcgtgagtcg
     1381 cgcacgcgcc ccagccgcca tgaccgctgt cgtggcaatc gaacagcgtg ccgttatcgc
     1441 gcttgccacg aaagcgcacg cccgagaagg tgccgaaatg cgcgccggtg acgcgctcgg
     1501 gcagcgcgtg ttccaaagct tttataatcg cgtcgatcac cgtcggaaac ggctgcgaat
     1561 aatttcccat cggcgccgtt ggatgggcgc tcaatatttt tccttcgggc agtatcagtt
     1621 tcagtggccg aaaagtgcct tcattggcgg gctcttcgtt ggcaattaga tacttgaagg
     1681 caacccgcgc cgtggtgcgc ccgccgccgt aatatcccga attgatgcag ccgcgcacct
     1741 gcccggcaat ccctgaataa tcgacggtca tctcgtcgcc ttcgatgatt actttgaccc
     1801 gaatcggcag cgcatcgtcg gtttcaccgt cattgtcgaa aaatgtttca taaccatacc
     1861 ggccgtccgg tatgcgccgg atgcgctcac gcgccgcagc ttcactctgg tcgagcatgg
     1921 ctttgagcgc ggctagaaaa gtctcgacgc cgtacttctc ggccaagctt tgcgtcagat
     1981 tgcgaccgag aatacaaccc gcgtgctgcg ccgcgatgtc gcccagaagc tcgataggaa
     2041 agcgcgtgtt gttttcgatg atccgataca cctcttgcat cggttcgccc ttggaccaca
     2101 gtttgatcga gcgcagctgt aggccctcca tgaagatatc gctggagcgc ggcgtgacgc
     2161 cgccgatatc gatccagtgg acattgatgg cgaaaaagcc aaccaggctg cccatctctg
     2221 cgccggcgta aataggcgta tacatcacgg tgttgttcaa gtgctggccc tgaatcgccg
     2281 cgtgattgca caggatcacg tcaccgcgct gcaagcgctc gcgaccatag acggcaaggc
     2341 catcgcgcac cgccatgccg accgagtcgg caacgaagac cggcatgccg ccggtgcatt
     2401 gggcaatgag ctcaccatcg gcgcttacca gcccgaccgc gaaatcttta tattcgtata
     2461 tgtaagggct aaaagctgtg cgcgcgatgt tcgcgtcgat ttgatcggtg atcgagacga
     2521 tgccgtgacg aatgacttcg agagtgatgg gatcgacggg tggccgcggt gctgtgagct
     2581 ctgccttggc tttcatcagc gtatcactca taagagctta agtatgaaag ctgaaagcgg
     2641 aaaactgaaa cggtaataaa gaaccgggtt ccgctttcat atttcagctt tcagctttga
     2701 tcttgatcct aatctcatgc agttcgccaa tttcgaaact gtcgcccggc cagaccagcg
     2761 tggtcgagcc gtactcttcg atcaccgccg ggcccgcgcc gctgactccc ggctccaggg
     2821 catcgcggtt atagatctgc gcatccgtcc aaccgccggc gctgcctata tacactcgcc
     2881 gcttctgcac cggctgggct ttgatcggcg cgcgtggcag ccgagcaatc tgcggttgct
     2941 tcaacttcgc aaaggcagag agatgcaacg cttgaaattc cgccggcgct ttggcgtcag
     3001 cgtggccata gcggcgcttg taatcgcgtt cgaaggcggc gcggatctct tgaacgtctt
     3061 tgagacctga aaccggaacc ttgatgttat gccgctggcc gcggaagcgc atctcggcgt
     3121 gatgttcgaa aaacgtttcg ctgctgctaa actcagcggc cagcgccacc gccgcatctt
     3181 tctccaaggc cgcgaagacc tccgccatgg tcgtgatcgt cttttcgtct aaaatcccca
     3241 cgaaagtttt tgaagtatcg atacgcgcat cggccaataa catgccgatg gcagaaaaat
     3301 ttcccggctc cggcggtatc acgactgccg gtatgtaaag ctcatgcgcc agcgcgcaag
     3361 catgcagcgg tccgccgccg ccatagctga agagaataaa atcgcgcggg tcgagaccgt
     3421 gctcgacgga aaccttccgt atcgcgccgg ccatgatcac cgtggcaatc gagataatgc
     3481 catcagccat ctggattgct ccgctttgac cggcgtatcc caacggctta gcgatacgct
     3541 tttcaatctc cgcctccgcg gctttcatat cgagcgccat ttcgccgccg agaaagttgc
     3601 cggcgttcaa gcgtccaaga attaaattag catcggtcac cgtcggttct gtgccgccgc
     3661 ggccgtaaca gaccgggccg ggtgtcgagc cggcgctctg gggtccgacg tgcaggcgat
     3721 tttgcgaatc gatccaagcg atggagccgc cgccggagcc gacttcgacg atatcgatga
     3781 ccgccgattt gatgggaaag ccttttacat agccgttggc gtagtaaacg gaattgactg
     3841 aaaagcggcc gttttcaacc agagcgcatt tggcagtggt gccgcccata tcgaaagcga
     3901 cgacattgtt gaagcccagc ttctccgcat aggcgctggc gccgatgcaa ccgccgatgg
     3961 gaccggattc gaccaagccg atgggttggc ggcaggtccg ttcggcagac agcaatccgc
     4021 cattggagcc catcatgaag agcgcgccgt cgaaaccttg atctttgaga tcttgatcga
     4081 gctgtccgat gtagctgcta acttgcggtc cgacgtacgc gttggcggca acggtggaac
     4141 agcgctcgta ctcgtaccat tcgcgcgtta ggtcggtgct gaaggtcacg taagtatccg
     4201 gcaacagttc tttaagcttc tcggcggcgg cttcctcatg cgccgggttg atgtaggagt
     4261 tcatgaagaa gatcgccagc gattcgatat cgagcatcct gatttcgccg gcaagtttct
     4321 ccagcgcttt taggtcgagc ggcgtgataa cttccccttt gctgctcatc cgttccggca
     4381 cttccaagcg aagctcccgc ggaatcaagg gctcgtcgcg ctggtaatgc aaatcgaacg
     4441 gatcgggccg gttgccgcgg gcaatttcca gaatgtcgcg aaatcccttg ctggtcacca
     4501 gcgcggtctt cgcgccctta cgttgtatca aggaatttat caccaaggtc gtgccgtgat
     4561 tgaccaggga cgcctcggcc ggcctgatgc tcgctttgtt gaaacaagca aggatgcctt
     4621 cgaccaaatt gtcgtagttg gtcgggctct tggcgtacgt cactttctga gtttgatggt
     4681 cgaaagcaac taaatcagta aacgtgccgc cgatatcgac ggcgatggca taggacattt
     4741 aatctccaag aagggatcaa ttagcgaaag ctgaaagcga aaagcagaaa tgaaacctcg
     4801 atcaaaggta aggcactaat tactagaatc tggcttcgtc gatgattgtt tggtgtgcaa
     4861 ggcttgcccc ggaaactatc aactgagaca cggcgtaaga gcgctgcaca tcggatgatt
     4921 ttaatttccg ctttcatctt tccggtttcg tccttgccgc tctcccctcc gcgtcgatcg
     4981 cgacgccgta attgtcgcgc gcgctttggc gagaaacgaa gccgcggata acatcttgtt
     5041 tgacccgttc gatggggcgc tcgcgggggt cgccgtagcc gccaccaccg cccgttgaca
     5101 tgcgcacccg atcgcctgct tgcaaggggc aattctcggt tttgtaaagc agctcgggtt
     5161 cctcgttgcc gttcttgaag atcaacacct ggccggactt ggcggcttta gcgccttgca
     5221 cgccccaggg cgggcagccg atgcggtcga agttcgccca cagttcgcag ggcgcaagca
     5281 gaatgtactg cttgtcgaat cccaggccgc cgcgccattt gccgggcccg cccgaatcct
     5341 gccgcagaca aaactcctcg acccggtaag ggtacatcga ctcttgcagc tcgatgggaa
     5401 tcagccgcgt gtcgccatgc gccatggtgc ggaacggccc cgcgccgtcg tgcgtcgcgc
     5461 acgcgcccca gccgccatgc ccgctgtcgt tagctttgaa caccgcgccg gtgtcggtgc
     5521 gtttgccgga aaaactcaac gaggaaaatg tgccgaaatg agcgccggta acccgctcgg
     5581 gcaacgccgg ttccagagct ttgataatgc aatcgatgat cgttggaaat ggaatgctgt
     5641 acagtcccat cgccgcagtc ggttccgcgc tgatcaattt tccttcgggc aaaatcatct
     5701 tgagcggccg gaaggtgccc tcgttggcag gctcgtccgt ggcgatcaga tacttgaacg
     5761 caacccgcgc ggtggtgcgg ccgccgccgt agtatcccga attgatacaa ccgcgcacct
     5821 gcggcgacat ctcggaataa tcgatggtga gctcatcgcc ggcgacgata attttcactt
     5881 tgatcggaat cttctcatcg cgcacgccgt cgttatccag gaacgcttca tgatggtaga
     5941 cgccgtcggg gatggcgcga atctttgacc gggtggcggc ttcgcactgg tcgagaatga
     6001 catcgagcgc gtgcagaaat acgccggcgc cgactttgtc cgccatcgtc tgggtaagat
     6061 cccggcccaa tagacagccg cccagttgcg cttcgatgtc gcccaacaat tccaacggaa
     6121 agcgggtgtt gttttcgatg atgcgataaa cctcttccat gggctcgcca ttggaccaga
     6181 tcttgatcga gcgcagctgc aagccttcca tgaaaatgtc cgtggacgag tacgataccg
     6241 aaccgatggc cgaaccgccg atgtcgatcc agtggcagtt aaccgcgaag aaaccaatca
     6301 actcatccct atgcgcgccg gcgaaaaccg gcgtgtacat aaccgtattg ttaagatgct
     6361 gtccctgcac cgccgcatga ttgcagacga tgacgtcgcc cctttgtaaa ttgtcgcgcc
     6421 catagatcgc caaaccatct ttgactgcca taccgactga gtcggcgacg aatacgggca
     6481 tgccgccggt ggattgcgcc aatagctcgc cgccggcccc gacaaatccc acggcgtagt
     6541 ccttgtattc gtaaatatag gggctgaatg ccgtgcgcgt catgttggcg tcgatctgat
     6601 cgcagatgga aatgatgccg tggcgaatca cttcgagggt gattggatcg atggttggtc
     6661 gggcaatggc
//
LOCUS       DASUVS010000145         3789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_710935, whole genome shotgun sequence.
ACCESSION   DASUVS010000145 DASUVS010000000
VERSION     DASUVS010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710935"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..1255
                     /locus_tag="VF182_08160"
     CDS             251..1255
                     /locus_tag="VF182_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6437091.1"
                     /translation="MVKAGTVGIALALCSLLVAEARGQAIDIAAAKKEGKVVVYGSVV
                     PQAMEELHKNFELKHGIRVDYWRGSSTAVAERAQSEWRAGRPAFDVVESSWDVMALMK
                     NEGIFVPYVPPSSQSFAEQFKEKDALITPWRILPISILYNTELVKAADAPKSLEDLLS
                     SKWKGKISMPDPSRHTTTAKFFWNLEKLMGAKWREYVKGLAKQQPLLLESLAPVTPAI
                     IKGEAQVGIAYIKFVKQYKGPVSYVPLDKYLSDPNHLALGAKASRSNAGRLYIEYAVS
                     AEGQRMIAQDGEFVLAPGVYPPIQGAEKVTPNMIPMDNPTEQEAKNLSSEFREIFFAQ
                     "
     gene            1336..2295
                     /locus_tag="VF182_08165"
     CDS             1336..2295
                     /locus_tag="VF182_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX6437092.1"
                     /translation="MEETVVYLNGAFVPESQAKVSVLDSGFNAGDGVYDVTRTFRHKP
                     FRLREHTERLFRSLQYTRIDCGMSLEDMEKTTLEVLERNKKLLRDNEDCALWQVVSRG
                     VRSMRGNRVSGRATVTVYPVIVNFPEFASFYVEGAPLVIPATRRIPPQCLESKAKITN
                     KMNHNMAAFEAKQADPRAIPLMLDIDGNLSETSAHNCFLVIDGKLCTPSDRNVLGGIT
                     KAAIFELAKQLGVEVVEGNYTPFDLYTAEEAFLASTSPTFVPIRTVNGLQIGKGAPGP
                     ITLRLIGAWNQMVGVDIVDQALSHLDKTEHDRLMTLWQSKKAA"
     gene            2479..3516
                     /locus_tag="VF182_08170"
     CDS             2479..3516
                     /locus_tag="VF182_08170"
                     /inference="COORDINATES: protein motif:HMM:NF040257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HEX6437093.1"
                     /translation="MNLSGNKKIRIIFYGVGAIGTEAAKFGLSRPGIEVVGAIDSDPV
                     KIGLDLGTVLGLEKDLGIKVTGDPAALFSEVEADVVVLTTGSFLPAIYDQLEAAVLAG
                     LNVVTSAEELAFPTLQSAELAEKIDQLAKKKGVTIKAAGINPGFVMDSLIVFLATASA
                     DIEHVSAKRVVDCSLRRRQLQMKVGAGLRVDQFRVSLGNKIFGHLGLMESAALVADAL
                     GMIPDRITQSVEPVIAEDNVESEHVRVKPGEVSGMRQVARCLSNGKECVSLEVLFCLG
                     ASEPQDQIVIKGMTDIDVTIKDGIAGDQATVAILINAIAPILEAKAGLLTPTGKSISE
                     ITWRCPVDLGY"
BASE COUNT          933 a          993 c         1072 g          791 t
ORIGIN      
        1 tgtatcgaca gcaccaaaga actatcgctt agccgtggat cacaggaccg atatttgaaa
       61 ctctgagcaa acactacttc aagaagtttt ttcattgacg gtctgacaat cgcgtgatct
      121 aacggtgctg atatcgcacc tgaaagcgta tacggggcag ttgcagtcaa cataacctcc
      181 ggccagaaaa ccaggcgcca tctaagaaag ccaagctggc gaactagctc tagcgaccga
      241 aggcatgaat atggtcaaag ccggcaccgt cggaatagct ctggctctgt gctcattgtt
      301 agttgccgag gcccggggcc aagcgatcga tatcgcggcg gcaaagaagg agggcaaggt
      361 ggtcgtctac ggcagtgtgg tgccgcaggc gatggaagag ctgcacaaaa attttgaatt
      421 gaagcacggt attagagtcg actattggcg cgggtcctcc accgcggtgg ccgagcgcgc
      481 gcaaagcgag tggcgcgcgg gccgccctgc atttgacgtg gtggagtcga gttgggatgt
      541 catggcttta atgaaaaacg aagggatttt cgtcccttat gttccgccgt cttcacaaag
      601 ttttgccgag cagttcaagg aaaaagatgc gttgatcacg ccgtggcgta tcttgccgat
      661 cagcattctt tacaacacgg aattggtcaa agccgctgat gctcccaaga gtttagaaga
      721 tctcctcagc tccaagtgga agggaaaaat cagcatgcct gatccctcgc ggcatacgac
      781 gacggcaaag tttttctgga atctcgaaaa gctgatgggg gcaaaatggc gcgagtatgt
      841 gaaagggttg gcgaagcaac agccgcttct gctggaatcc cttgcgccgg taactccggc
      901 catcataaaa ggagaagcgc aggtcggcat cgcctacata aaattcgtca agcaatacaa
      961 aggtccggtt tcttatgtcc cgcttgataa atatctctcc gacccaaatc acctggcgct
     1021 tggagccaaa gcgtcgcgct caaacgccgg ccgactctac attgagtacg cagtgtccgc
     1081 cgaagggcag agaatgatcg cacaagacgg cgaattcgtc ctcgcgcccg gtgtttaccc
     1141 gcccatccaa ggcgctgaga aggtgacgcc gaacatgata cccatggaca accccacgga
     1201 gcaggaggca aagaatttaa gtagcgagtt tcgcgagatc ttctttgccc aatgaatcac
     1261 cggcggcgca tcgaagtgaa cagcacaatc gtaattcagt tgcttctcta gaaccgacga
     1321 aaggaagata aagcgatgga agagacggtc gtttacctga acggggcatt cgtgccggag
     1381 agccaggcaa aggtgtcggt gttggacagc gggtttaacg ccggtgacgg cgtctatgat
     1441 gtgacacgca catttcgcca taaaccgttc cggctacggg agcataccga gcggctgttc
     1501 cgctcgcttc aatatacccg catcgattgc ggcatgtcgc tggaggatat ggagaagacg
     1561 acgttggagg tcttggagcg caacaagaag ctcttacgtg ataacgaaga ctgcgctctc
     1621 tggcaagtcg tgagccgggg agttcgctcc atgcgaggca atcgagtgag cggccgcgcc
     1681 accgtgactg tctatcctgt gattgtcaat tttcccgagt ttgcgagttt ttatgtcgaa
     1741 ggcgcgccgc tggtcattcc tgccaccaga cgcataccgc cacagtgcct cgagtccaaa
     1801 gcgaagatca ccaacaagat gaatcacaac atggcggcgt tcgaggccaa gcaggccgat
     1861 cccagggcga ttccgttgat gctcgacatc gatggcaatc ttagcgagac cagcgcgcac
     1921 aattgttttc tggtcatcga cggcaagctc tgcacgcctt cggacaggaa cgttctcggc
     1981 ggcattacca aagcggcgat cttcgagctt gccaaacagt tgggcgtgga ggtcgtcgag
     2041 ggaaactata cgccattcga tctgtacacc gccgaagagg cttttctagc cagcaccagc
     2101 ccgacattcg ttccaatcag gacggtcaac ggtctgcaaa tcggcaaggg tgcgccggga
     2161 ccgataacgc tgcgattaat cggcgcctgg aaccaaatgg tcggagtgga catcgtcgac
     2221 caggccttga gccatctcga caagacggag cacgatcgcc tgatgactct gtggcagagc
     2281 aagaaagccg cctagcttag caggctgctg aaaacactca tatgcttcgt tgcgttcgcc
     2341 cgattcgctt caacgtactg aagcgtacgc ctcggctcat cgaacttcgc gcgccttgcc
     2401 tctgagatct tttgagcagc ctgcaaacac agtttttcat tactgtgcaa atccattcca
     2461 cacgcagttg cgcttcatat gaacctttcc ggcaataaaa aaataaggat tattttctac
     2521 ggtgtcggcg cgatcggtac tgaggcggct aaatttggtt tgagccgccc agggatcgag
     2581 gtagtcgggg ctatcgattc tgatccagtc aaaattggac tcgatctcgg cacggttcta
     2641 gggctcgaaa aagacctcgg aataaaagta accggcgatc ccgcggcact cttcagcgag
     2701 gttgaagcgg atgtggtcgt gctcacaacc ggttccttcc tgccggcaat ttacgatcag
     2761 ctagaggccg cagttctggc cgggctcaac gtagtcacct cggcagaaga gctcgccttt
     2821 ccaactctgc aaagcgccga gctggcggag aagatcgatc agctggccaa gaagaagggc
     2881 gttacgatca aggccgccgg tatcaatccc ggtttcgtga tggacagcct gatcgtattc
     2941 ctagcgacag cgtcggccga tatcgagcat gtgtcggcga aacgggtggt ggattgctcg
     3001 ctgcgccgca gacagctgca aatgaaggtc ggagcgggcc tgcgcgtcga tcaattcagg
     3061 gtcagtctcg gcaacaaaat tttcggccat ctgggactaa tggagtcggc ggcgctcgtg
     3121 gctgacgcac tcggtatgat tcccgatcga attacccaat ccgtcgagcc ggtgatcgcg
     3181 gaagacaacg tcgaatcgga acacgtaaga gtaaaacctg gtgaggtttc cggcatgcgc
     3241 caggtcgcgc gctgtctaag caacggcaag gaatgcgtca gcctcgaagt gcttttttgc
     3301 ctgggagcgt cggagcccca ggatcagatc gtaatcaaag gaatgacgga catcgacgtc
     3361 acgatcaaag atggcattgc cggtgaccag gccaccgtgg cgattctgat taacgctatt
     3421 gcgccgattt tggaagccaa agccggactc ctgacgccaa ccggaaaaag catctcggag
     3481 attacctggc gctgccctgt ggatttaggc tactgacccg tacgcgatcc gttgtcattg
     3541 aaaagtttct aggtaattgt ttccgcctgc gacgcgcgca accttcgaca tgaatgccga
     3601 aagcacaata cagaaagagc taaaggcacc gttcgaagca gcaggtcgaa cgatgtggtt
     3661 acccgttcct tcgaatctca gtgaaacacc gagactccga gaattctggt tcctcaatcg
     3721 gcagctatgt caaagcgtga cggaaacaag cgacagatac tgaaacagat ggtgagagct
     3781 aggttgctg
//
LOCUS       DASUVS010000146         7535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_492500, whole genome shotgun sequence.
ACCESSION   DASUVS010000146 DASUVS010000000
VERSION     DASUVS010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492500"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            468..1199
                     /locus_tag="VF182_08175"
     CDS             468..1199
                     /locus_tag="VF182_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010043428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6437094.1"
                     /translation="MRLWVGTSGFGYKEWRGKFYPQRFPAKEMLGFYASRFAAVEINN
                     SFYRLPKETVLRSWAEQVPAEFRFVLKAPRRITHTKRLKDAGVEVDDLYSVAQALGSS
                     RGAFLFQLPPNFKKDLDRLRAFLALVPADQHVAFEFRHPSWMDEAVFASLRTRNSALC
                     VTDTDEGDDCDLISTASWGYLRLRRREYTGADLLRWKKRICSQAWDHAYVFFKHEDEA
                     TGPKFAAAFSNTPACPRDGPSLDEG"
     gene            1233..1766
                     /locus_tag="VF182_08180"
     CDS             1233..1766
                     /locus_tag="VF182_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX6437095.1"
                     /translation="MCTVITHPAIPIALSVLLPQESASSSLLIAGALCSVVPDLDVIG
                     FDFGIKYGDLLGHRGFTHSIFFAALFAALMTMFLFQNHGSRLIVLLFLFLATLSHPVL
                     DALTNGGLGVGLFAPFTNERYFFPYRPIEVSPIGVGAFFSHRGLKVMLSELRWVWLPS
                     TVFFGLGLLSKRLRDLT"
     gene            2038..2343
                     /locus_tag="VF182_08185"
     CDS             2038..2343
                     /locus_tag="VF182_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437096.1"
                     /translation="MKRSEPGLLQGFFLCLRDSEAIRLQSSKWKRSLLIFSSGICLEV
                     AAVPYLLGWIGFEATWQAWLLTWLFFPLGVIGAYASIFGGDRLVEWLLIVPKLDLKM"
     gene            complement(2478..2735)
                     /locus_tag="VF182_08190"
     CDS             complement(2478..2735)
                     /locus_tag="VF182_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437097.1"
                     /translation="MRKGISLGTAIVLAGFLGITLPGCSKDRNSSGTGGDTGKSSGSA
                     TGSKSSPGGGSSSTGAGGSATGSGSSGSAGGSGTGSAGSGS"
     gene            2852..2974
                     /locus_tag="VF182_08195"
     CDS             2852..2974
                     /locus_tag="VF182_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437098.1"
                     /translation="MAQAESAEWGMGEALAVVGQDWELDRAPGPAGGERHGLSI"
     gene            complement(2993..3442)
                     /locus_tag="VF182_08200"
     CDS             complement(2993..3442)
                     /locus_tag="VF182_08200"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6437099.1"
                     /translation="MKTVNKAIELNSPVHRVYSQWTRFEDFPRFMHGVKKVILLDDRR
                     LQWETEFSGMTKEWYATITDLTPDQRIAWESEDGEYVAGIVTFQPIGTTGTSLNLKLF
                     YDSKGSRRAEEEFVSSAGDRVAQDLERFKKFIEEQTNVNNRPGGIRP"
     gene            complement(3636..4739)
                     /locus_tag="VF182_08205"
     CDS             complement(3636..4739)
                     /locus_tag="VF182_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6437100.1"
                     /translation="MTPDGIKRIIDLLTDAFILLSDSGEILTANAAAHELFQTDHLAG
                     LALADLVTDPASTVSDWLNRSSASRQMIAASLNLRADDTSIPVGCEGGLLQAGDTINP
                     ALILVHCMRRNEASNADLLENLKSGLDSLQKQLVQQKAQDSQQIIMLRTAAAVFAHEI
                     ANPLNAVSTCMQILQAKNERHNDRDSVVHAMIESASAEIERLTRLLNDFRAFARPQTI
                     DFRPTLLSKIIEEALTLEMASLTASGIAVDIDLRPLPALMLDGQKMREAMLNLFKNAE
                     EAMPAGGRLTITGHLREQEKVAVVEVRDTGTGVPPGVDVFQLFMTTKPGASGLGLPIA
                     AQIIASHGGSIHQVADRTPGTTFEILLPLPRDS"
     gene            complement(4806..5171)
                     /locus_tag="VF182_08210"
     CDS             complement(4806..5171)
                     /locus_tag="VF182_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6437101.1"
                     /translation="MARSVLLVEDSSSARRNIAIFLERAGYQVAQADTGEDAVRLIES
                     NGTFDVVVSDLRMPGMINGLDVLSFHNRIAPHTGSILMTAFGSDQIRDRTSQLGAAYF
                     EKPVKLSSLLLEIEKHARR"
     gene            complement(5288..5749)
                     /locus_tag="VF182_08215"
     CDS             complement(5288..5749)
                     /locus_tag="VF182_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX6437102.1"
                     /translation="MNALDLLTDDHQKVRQLFERVQQIRDNDEKKELFDQIDTELAVH
                     AEIEETIFYPALEEHDELKDMVREAREEHEQVEQLLLEIEDLATEDTDFTSQLVELEE
                     TVEHHVAEEEEEMFPKVREIFDKAALEQLGKELASAKGKQMNPGKDDIQIA"
     gene            complement(6195..6743)
                     /locus_tag="VF182_08220"
     CDS             complement(6195..6743)
                     /locus_tag="VF182_08220"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX6437103.1"
                     /translation="MTSQKLLSMEAFLDGIDEILGHGFATADIHAHLQSTLIAPDSLE
                     RYVFFRPERYTRNLVFKNEFFELLVICWNIGQRAPVHGHEGERCWSRVEQGSLLLCNY
                     HEVSENPLVVRQIGEPYLGERGHLDGPADIHSVANPSNVPAVSLHLYSHPYDQCDIYD
                     LDKNLKQRVRLRYDSIDGKIVA"
     gene            complement(6755..>7535)
                     /locus_tag="VF182_08225"
     CDS             complement(6755..>7535)
                     /locus_tag="VF182_08225"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6437104.1"
                     /translation="AGNEKLKKLARLVGRMKFHAMALRKKAFERSNEEVLEVERGDSI
                     HRLLPHEMLTLHHPTLRKDFFRRYLDQELIQYSLRGVEEKGKGPMIVCLDGSSSMAGD
                     KEIWSKAVTLTLLEIARRQRRLFRSICFSSEETPLQILDMNSRDHYEIETKSVMDLAE
                     YFPGGGTDFQRPLDAALECLKHARFKKGDVVFITDGECQVAPQWAEEFRKEKERLGFS
                     LFSILIDVGSASLGTLTEFSDRITTIKQLTGDEAKDIFVKF"
BASE COUNT         1623 a         2014 c         2019 g         1879 t
ORIGIN      
        1 acagtgggcc tgcggcaggg gagcctggat tgttatgtcg cccactccgg tcatacttcg
       61 accttgccgg gttcggccag atgtttccgt ccaatagtag acaagtacaa aagatttctc
      121 gtgaggcccg gttaaccgta agttattgtt ccaattttcc ctgtgaggag ggaactgaaa
      181 gagactaagc gccggagtac ttttcgaata atttgccgag tcgcaaacat tttccctgtg
      241 aggagggaac tgaaatgcga ttgcatgccg tccccggtaa tgcactggtt cttttctgca
      301 gtcctcggcg cattcttggc gcggatgcaa gttcgcgctt cagccgagtc gtctccccac
      361 tgagatgaca aactaaacgt cctgtctcca cgctcgatct atgataaata taatccaatg
      421 ccggatgacg ccgcgctaaa tgtcgatctt aaaaggcgcg agtacacgtg aggctctggg
      481 tcgggaccag cggctttggc tacaaggaat ggcggggaaa gttttatccc cagcggtttc
      541 ccgccaaaga gatgctgggc ttttacgctt cgcgattcgc cgctgtcgag atcaacaata
      601 gtttttaccg gctgcctaag gaaactgttc ttcggtcgtg ggcagagcag gtgccggccg
      661 agttccgatt tgtgctgaag gcgccgcgaa gaatcacgca caccaagagg cttaaagacg
      721 ctggagtgga agtcgatgat ctctatagcg tggcgcaagc gctaggatcg agccgaggcg
      781 cctttctctt tcaattgccg cccaatttta aaaaggacct cgatcgactg cgagcattcc
      841 tagcacttgt gcccgctgat cagcatgtgg cctttgagtt ccgacatcca tcctggatgg
      901 acgaggcggt ctttgcgtcg ctgcgaacac gcaacagcgc gctttgtgtc accgacacgg
      961 atgaagggga tgattgtgat ctgatcagta ctgcgagttg gggttatctg cgattgcggc
     1021 gtcgagaata cactggcgcc gatctgctgc gctggaaaaa gcggatttgc tcgcaggctt
     1081 gggatcatgc ctatgtgttc tttaaacacg aagacgaagc taccggacct aaattcgctg
     1141 cagcattttc gaacaccccg gcttgcccgc gtgatggccc gagcctcgat gagggttgag
     1201 ctttgcccgc cgttggtgcg aagatcagcg ccatgtgcac agtcataact catcctgcta
     1261 tacccatcgc attatccgtg ctgcttccgc aggaaagcgc ttcgtcttct ttattgattg
     1321 ctggagcgct ttgctcggtg gtcccggatt tggacgtcat cggctttgac ttcggaatca
     1381 aatacggcga tttgctgggc caccggggtt ttactcattc gatattcttt gccgcgttat
     1441 ttgccgcatt gatgacgatg tttctgttcc agaatcatgg cagtcgatta attgttcttc
     1501 tgtttctctt tttagcgaca ctgtcgcacc cggtgctgga tgcgcttaca aatggcggct
     1561 tgggagtcgg tttattcgct ccattcacca atgaacgata tttcttccct taccggccga
     1621 tcgaggtttc gccgatcgga gtcggtgctt tcttttcgca cagaggtctt aaagtgatgc
     1681 tgagtgagtt gagatgggtt tggttgccgt cgacagtctt ctttgggctg ggccttctgt
     1741 ccaagcgctt gcgcgacttg acctgagcac ggcttgtatt tagtcaaacg ggtagtctgc
     1801 tgcaaaattc tttgcttcga agaagcgtaa tttcgtagga ccatcgatcg tggcaattcg
     1861 tacctccggc tcgtacccca gatgacgcct tgctcatccg ctccagagaa acatttccag
     1921 gaatctaagc ctgggaattt gcattatcat cgatagcgct ctaccattgc gcctcgtgcg
     1981 atccacccgc caggcagtgg catccaatcg caaatatcgg tgaaaggttt cacaaagatg
     2041 aagcgttccg aaccaggttt actgcaagga ttttttctgt gccttcgcga ctcggaagcg
     2101 atacgtctgc agtcgagtaa gtggaaaaga agtcttttga tctttagcag cggcatctgt
     2161 ctcgaggttg ccgctgttcc ttatctgctt ggttggattg gatttgaagc gacgtggcag
     2221 gcgtggcttc ttacatggct attcttcccc ttgggagtta tcggagctta cgcgagcatc
     2281 ttcggcggag atcgcctggt cgaatggctg ttaatagttc ccaagctaga tttgaaaatg
     2341 taatcggcga gacgccacca gcccacgtgt tgcactcacg agacttcatc gcgcccatcc
     2401 gatgtaagtc tcgcacatga gctggcttgc ctagtttagt agcgcaacgg ctcgacctat
     2461 tactttgtcg agctagtcta tgatccgctt cccgcagagc cggttccgct gcccccggct
     2521 gatccgctgc ttcctgaacc cgtagcgctt ccgccggccc cggtcgagct agaaccaccc
     2581 ccgggactcg atttagaccc ggtagcgctg ccgctgctct ttcccgtatc tccgccggtt
     2641 ccggacgaat ttctatcctt cgagcacccc ggcaatgtaa taccaagaaa tcccgccaaa
     2701 acgatcgccg tgcccaatga aatccctttt ctcatggttt cctcctttcg ctcgggtctc
     2761 gccgttacgc ttgctctact aaaagcaacg catatgccat tgagtgacat agaatgcatc
     2821 tgcggcggtt atcgattaag cgcgtaaacg ggtggctcag gcggaatcgg cggagtgggg
     2881 aatgggtgag gcgctggctg tggtgggtca ggattgggaa ttggaccggg ccccgggtcc
     2941 ggccggtgga gaacggcacg gcttatcgat ttagtgaccc ggctcatgga gttcaaggcc
     3001 gtatccctcc gggccgattg tttacgttgg tttgctcctc gatgaatttt ttgaatctct
     3061 ccaggtcctg ggcgacgcga tcgccggcag acgatacaaa ttcttcctct gctcggcgcg
     3121 agcctttgct gtcgtagaac agtttgagat tcaggctcgt tccggttgtc ccgatcggct
     3181 ggaacgtgac gatccccgca acgtattcgc catcctcgct ttcccatgcg atccgttgat
     3241 ccggggtcag atcggtgata gtcgcgtacc actccttcgt catgccgctg aactctgtct
     3301 cccactgaag ccgccggtcg tcgagcagaa tcaccttttt gaccccatgc ataaagcggg
     3361 ggaagtcttc gaatcgggtc cattggctgt aaacccggtg aacgggagaa ttcagctcaa
     3421 tggctttgtt taccgtcttc atttctgtca cagaaacagc aatggaaatg ccattcattg
     3481 tgctgcgtac gttgccggga ccagcgttgc aaagagtgca cagacagatg gaagctgtcg
     3541 cacacctaag actggtcgcc agatcagcag ccgatgatgg tcgtgctacg atgcaggagg
     3601 atcttttgag tgtttcgtga attcatcagg aaggattacg aatcgcgcgg aagcggcagt
     3661 aagatctcga aagttgttcc cggagttcgg tctgcgactt ggtgtatgct cccgccgtgg
     3721 gacgcaatga tctgcgcggc gatcggcaag cccaagccgg atgcgccggg tttagtggtc
     3781 ataaataact gaaaaacgtc gactcccgga ggtacaccgg tgccggtatc gcgcacctcc
     3841 acaacagcga ccttttcctg ttcccgcaga tggccggtga ttgtcaggcg gcctccggcg
     3901 ggcatcgctt cttcagcatt tttaaataag ttcaacatag cttctctcat tttttgccca
     3961 tcgagcatta gggccggcaa tggtcgcaaa tcgatatcaa cagcgatccc gctggcagtt
     4021 aagcttgcca tttcaagcgt gagcgcttct tcgatgattt tacttagaag cgtgggtctg
     4081 aaatcaatgg tttgaggacg ggcgaaggcg cggaagtcgt ttagcagtct tgtcaggcgc
     4141 tcgatctcag cggatgcgct ttcgatcatc gcgtgcacaa cggaatctcg atcattgtgg
     4201 cgctcatttt tcgcctgaag aatttgcatg caggtcgaaa ctgcatttag cggattggcg
     4261 atctcgtgtg caaaaacggc ggcggcagtg cgcagcatga tgatctgttg gctatcctgg
     4321 gctttttgct gaaccagctg tttctgcagt gaatccaacc cactttttaa gttctccagc
     4381 aagtcagcat tcgacgcttc gttcctgcgc atgcagtgaa ccagaatcaa ggcagggttg
     4441 atggtatcgc cggcttgaag gaggccgccc tcgcagccca ccggaatgct ggtgtcgtct
     4501 gcccgcaagt taagtgatgc ggcgatcatt tgccggcttg cgctcgaacg gttcaaccaa
     4561 tcagagaccg ttgatgcggg atcggttact aaatcagcca aagccagacc agcgagatga
     4621 tccgtctgaa acaattcatg ggctgccgca ttggccgtta ggatctcccc tgaatcggag
     4681 agcaaaatga aagcgtccgt taaaaggtcg atgatccgct taattccatc aggggtcaat
     4741 ggttccagct ccaacgccac atcgtaattt tttacatctg cgttgcgtcg gctggagttg
     4801 ccgggctaac gccgagcgtg tttttcaatc tccagaagaa gtgaactcaa cttgaccggc
     4861 ttctcgaaat aagcagcgcc gagctgactt gtccgatccc ttatctggtc ggagccaaaa
     4921 gccgtcatca gaatcgaccc ggtatgggga gcgatgcgat tgtgaaaaga cagaacatcc
     4981 aggccgttga tcatacccgg catccgcaaa tcgctcacta ccacgtcgaa agtgccgtta
     5041 gattcaatca agcggacagc atcttctccg gtatccgcct gagctacctg gtaaccggcg
     5101 cgctcgagaa atatcgcaat attccggcgc gcagacgagc tatcctcgac caataaaacg
     5161 ctcctagcca tgatccgtac ctccacacat ggcgctggcg ctcacagctc tgaagcagga
     5221 gagaaatgtc cggtaaacat cgtgccggca gtgaatgaac agcgggtgga acctacgagt
     5281 gagtatctca ggcaatctgt atatcatctt tgccgggatt catctgcttt cctttggctg
     5341 atgcgagctc ctttcccagt tgctcgagag cagccttgtc gaaaatttca cgaactttgg
     5401 gaaacatttc ctcttcttcc tcagctacat gatgttcaac ggtctcctct aattccacga
     5461 gctgggaagt gaaatcggta tcctcggtcg ctagatcttc gatctctaga agcagctgtt
     5521 cgacctgttc gtgctcctcg cgcgcttccc gcaccatatc tttgagctcg tcgtgttcct
     5581 ctaaggcagg atagaaaatg gtttcttcaa tctcggcgtg gacagccaat tcggtgtcga
     5641 tctgatcaaa cagctctttt ttttcgtcgt tgtcacgaat ctgttgcacc cgctcgaaga
     5701 gctgcctcac cttctgatga tcatctgtaa gcagatccaa ggcattcatc tgcacctcca
     5761 tgcgactgga atagaccagg tccgagcaaa gcaggtgcca cagaataggt gagaaactct
     5821 gttgctgcgg ttgaaggata gtagcctatc gtccgattga tgatttgtga aagcgtgtga
     5881 tcgtcccatt cgcaagacag ggggacttcc ctctgcataa ctcacttaac gcaggattga
     5941 actctgttac cccaggagta gccggagtag cgtccgatca taactgcctc ggagttatcg
     6001 acgagtcatg ggatttcaat ccgcgggtgg gcagagtcga tactgcagta ggcggcaaac
     6061 acagaatgca gagggctgcc gcatctcgag ttgcagccaa tggtgctcgc cctggctatg
     6121 gctgatttga tctgatctgt agccgggctt cgctctcgaa tgacagcaca ggtcggcctg
     6181 tactgggtca cctatcaggc gacaatcttg ccgtcgatgc tgtcgtaacg tagccgtacg
     6241 cgctgcttca agttcttatc gagatcgtag atatcgcatt gatcgtaggg gtgggagtag
     6301 aggtgtaggc taaccgccgg cacgtttgac gggttggcca cgctgtgaat atccgctggg
     6361 ccatcgagat gaccgcgctc gccgagatac ggttcgccga tttgccgcac caccagcggg
     6421 ttttcggaaa cctcgtgata gttgcacaat agcaggctgc cttgctcgac ccggctccag
     6481 cagcgctcgc cttcatggcc gtggaccggc gcccgctggc caatgttcca gcagataacc
     6541 agcaattcga agaactcgtt cttgaatacc agattccggg tataacgctc cggccgaaaa
     6601 aaaacatacc gctccaggga atcgggtgcg atcagcgtgc tctgcagatg agcatgaata
     6661 tccgcggtgg caaacccgtg gccgagaatc tcatcgatac cgtccaggaa agcctccata
     6721 ctaagcaatt tttgactggt catggtacga gcctctaaaa cttcacgaaa atatctttgg
     6781 cttcgtcccc ggtgagttgc ttgatcgtcg taatgcggtc gctgaattcg gtgagcgtgc
     6841 cgagcgaagc agagcctaca tcgatcagaa tcgagaacaa cgaaaagccc aagcgctcct
     6901 tttcctttcg gaattcctcc gcccattgcg gcgcgacctg acactcgccg tcagtgatga
     6961 acacgacatc gccttttttg aaccgcgcgt gcttcaagca ttccaacgcc gcgtccaatg
     7021 gccgctggaa gtcggtcccg ccgccgggga aatattccgc aagatccata acgcttttgg
     7081 tctctatctc atagtgatcc ctggaattca tatcgagaat ttgcaaaggc gtttcttcgg
     7141 aagaaaagca aatcgagcga aagagcctgc gttggcgtct ggcaatctcg agcaatgtca
     7201 gagtaacggc cttggaccag atttccttat cgccggccat cgaagagctg ccgtcgagac
     7261 agacgatcat cggacctttg cccttttctt cgacgccgcg caatgagtat tgaattagtt
     7321 cctggtcgag ataacggcgg aaaaaatcct tgcgcagcgt gggatgatgc aaggttaaca
     7381 tctcgtgcgg cagcagacgg tgaatcgaat cgccgcgttc gacttcgagc acttcttcat
     7441 tggatcgttc gaaagccttc ttacgcaacg ccatggcatg aaacttcatc cgccccacca
     7501 ggcgcgcaag ctttttaagc ttctcgttgc cggcg
//
LOCUS       DASUVS010000147         5541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_274410, whole genome shotgun sequence.
ACCESSION   DASUVS010000147 DASUVS010000000
VERSION     DASUVS010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274410"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..964)
                     /locus_tag="VF182_08230"
     CDS             complement(41..964)
                     /locus_tag="VF182_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437105.1"
                     /translation="MQIRIAAVDLISNTCFPALAADELGFFKAEGLDARIELVAALGA
                     TRALHDGSADAMIAGSVHDVLTVFPQWNGVKIVVALSKGTPWLLVIRADLSAKRGDLS
                     ATKGLRLTAAEGPDLALRQMLIGAKVDPDRDLQIIQLPGAKARDVSFGVFAARALEAR
                     QIDGFWANAMGAETAVSRGVGKVLIDVRRGDDPGEVRRFTFAGMVTSDTFIENHSAVV
                     AAAVRAIVKTQKALKADPGLAVQVGRRKFPKEAAELIGKIVERDRVFYDPVILPEEVE
                     AMNRFAQSVGHLSVAVPYDKVVATPFQNLWQ"
     gene            complement(1257..1733)
                     /locus_tag="VF182_08235"
     CDS             complement(1257..1733)
                     /locus_tag="VF182_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437106.1"
                     /translation="MFVETYRQENSGSIMAGGDARPIPYPKISQEDWRVWTLFLPVRS
                     ANINRISARAIKEESLHFSYGIPYALTEEVQKATELFDKVEVWRKRDVSKDPIAVGVL
                     DGERYLIARWGMEKLLPFESIKKAVPLILAWKYATGPAGAMAGLVGMGFLAWGMLF"
     gene            2440..3156
                     /locus_tag="VF182_08240"
     CDS             2440..3156
                     /locus_tag="VF182_08240"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEX6437107.1"
                     /translation="MFREKVELKDLERIDSEYRDLLTRVVTIQADCEIGGPHLYAKDI
                     LPSAPTKTDQLLVARTASEELDHYRKIARVAGDFGVDVSFVLSWPNKKRYLEAFRGTI
                     NTWEDFAVFGFLIDRVGRYQLEEFFGCTYQPLTEILPIIIKEEIGHVGYGESKTAELA
                     AKGEESREKVQAALDRWYVKALDMFGRSDSTRDERYIYWGLKRRTNEQARQEFIRELD
                     PLIEKIGLRVPDPMQGRQYF"
     gene            complement(3183..5093)
                     /locus_tag="VF182_08245"
     CDS             complement(3183..5093)
                     /locus_tag="VF182_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6437108.1"
                     /translation="MPLLRLDDISLSYGYLPLLAHVQFQIEAGERVCLVGRNGTGKTT
                     LFRVITGDAVPEAGNVRAHEGLRVAHLEQDVSPDKERTVFQVVASGLGELGRLLFEYR
                     QATQEAGAAEAPSLEALANLDARIESLGGWNLNQKVETVLTRLSLPPDKPIEACSGGV
                     RRQVMLARALVGEPDLLLLDEPTNHLDISTIIWLEKFLLNYRGALMFITHDRSFLRHL
                     ATRIVELDRGTLTSYPGDIDAYVRKKDELMAIEERAAAKFDKKLAEEEAWIRRGVKAR
                     RTRNEGRVQALQQMRRERSRRLDSQSRASFSIDAGALSGKLVVDLRRVSFAYENDLVV
                     REMSTRILRGDRVGIIGPNGSGKSTLLKLILGEIRPSSGEVVMGTRLRLAYFDQHRRI
                     LDPEKTVRENLSDSDYVTVQGRSHHVIGYLKDFLFPPQRIDSPVKALSGGERNRLLLA
                     RIFTQPANMMVLDEPTNDLDVDTLELLEDLLAGYRGTLLLVSHDRTFLDNIVTSTLVF
                     EGAGKFVEYAGGYADWVRCQELSRASQAKTPNRTPAAAIAASKDPPGKNNSKLSYKEK
                     RELEALPARIEVLETEQSELQLRTAQADFYRQPGAAITSALERLEALGNELDECYARW
                     QSLESHSSARGN"
     gene            complement(5239..>5541)
                     /locus_tag="VF182_08250"
     CDS             complement(5239..>5541)
                     /locus_tag="VF182_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437109.1"
                     /translation="IAALPELAESVDYIVFGNNAGQGFPLAQSLPGNLIDDHAAIIYA
                     DFLPQMSRYQKLGFRNFFRRNEATKRLVDLATKSQRPLELLFINTIQHNETNYHNP"
BASE COUNT         1319 a         1494 c         1413 g         1315 t
ORIGIN      
        1 tcatacagca cggaggtggt tgtataccgt gatactcgag ttactgccaa aggttctgga
       61 aaggcgtcgc aaccacttta tcgtacggta cggcgacgga taagtgaccc acagattggg
      121 caaatcggtt catcgcctcg acctcttccg gcaatataac gggatcataa aaaaccctat
      181 cacgttcaac gattttccca attaactccg cggcttcctt aggaaactta cgcctcccca
      241 cttgcaccgc gaggccggga tctgccttta gagctttttg tgttttcact atcgcgcgaa
      301 ccgccgccgc gacgacggcc gagtgatttt cgataaaggt atcgctggtc accatcccgg
      361 caaaagtaaa gcgccggact tctccgggat cgtcgccccg gcgcacatcg atgaggacct
      421 tgccaacacc gcggctgaca gcggtctcag cacccatcgc attggcccag aacccgtcaa
      481 tctgtcgtgc ttccaatgca cgggcggcaa acaccccgaa cgaaacatca cgcgctttag
      541 cgccggggag ctggataatt tgtaaatctc gatcaggatc aaccttagcg ccgatcaaca
      601 tttgccgcag cgccaggtcg gggccttcgg cagccgtaag acgaagtccc ttggttgcgc
      661 tgaggtcgcc acgtttggcg ctcaggtcag cacgaatgac gagcagccaa ggagtccctt
      721 tcgaaagagc cacgactatc ttgactccgt tccattgagg aaacaccgtc aacacatcgt
      781 gcaccgagcc ggcgatcatt gcatcggcac taccatcgtg taaagctctt gtggcgccta
      841 atgctgcgac caactcgatt cgcgcatcca gcccctccgc cttgaagaaa cctagctcat
      901 ccgcagccaa tgccgggaaa catgtgttac tgatcagatc gactgctgca atgcgtattt
      961 gcaagatgtc ctcctagcaa cattgggaag atcttgccga aactagcata ttctctggtg
     1021 gatcaatagt cgattctgct cgacagattg aaagtttaaa ccgcagaatc taaacgactg
     1081 ggtcggcggt gcttgctcgg tggctgcaag caatggaggg cagaccgttg ttcccctcgg
     1141 taagcagtct gggccgaacc tcggtattga tgtggttcta agttttgctg aaagcggtta
     1201 gagagacccc atgggaatca ctcgaaccgc tttcattgtt tctattcgat cgaagcctag
     1261 aatagcattc cccaggccag aaaacccatt ccgactaagc cggccatggc gccggcggga
     1321 ccggtcgcgt acttccaagc cagtataagc gggacggctt ttttaatact ctcgaagggc
     1381 aataacttct ccattcccca acgggcaatt agatagcgtt ccccgtcaag cactccgacc
     1441 gcaatcggat ctttactgac atcccgtttg cgccatactt cgactttgtc gaataactcc
     1501 gttgctttct gtacctcctc tgtgagcgca tacggaattc cataagaaaa gtgcagcgac
     1561 tcttctttga tggcccgagc cgaaattcta ttgatgttag ctgatcggac cggaagaaag
     1621 agagtccaga cgcgccaatc ctcctgagag attttcggat agggtatcgg tctcgcgtcc
     1681 cctcctgcca ttatggatcc gctgttttcc tgcctatagg tttcaacgaa cataatttca
     1741 cctccctctt ttattttttg atctatttaa attttcgtat gatcaatgcc ctactctcgt
     1801 agtcattgga ggatggcgag ttgccttcag aaatggcgca aattttatac cagcaaaatt
     1861 ggccgatccc tcgtaagcgt cccgtcaatc tcgggacaaa tcgtcgaccg ctgtgtcaat
     1921 tgcgagacag taatttgcca tcgagctcga gtccaatggg gggaagtggt aaactgaact
     1981 aggctcttcg catacgcatt ttgcctgaat gacgagtatc tgaaacttaa ttttcgtagt
     2041 ctaagccgct ttgttaactg ccgtttagct tctaaagatt gaaattgttt accgtctgaa
     2101 ctctatttga ctcaagtgca agcaggcaaa gagatcttgc tgtatcagcc gtgagatttt
     2161 agttgtttcg tgccgatcgt cagcgaaaaa tctgctctcg cttgagcgcg aagctcctct
     2221 gcggttcgga gcgcggcccc gtagccatcc ttctggcata gaaagtgcgg ttttccattg
     2281 atatcgatag caaatatcaa gcagaagatc gtatccaact ttgagaagtg tcacgtgaat
     2341 tttactgaag gtgtctggct taatggaaag aatgcttccg cttcagcgta tccaatcgcg
     2401 aacacaccgg agtttgtcga gaagtaaaat ggaggaccaa tgtttcgtga aaaagtagag
     2461 cttaaggatc ttgagcgaat agactctgaa taccgcgatc ttttaacccg tgtcgtaaca
     2521 atccaagcgg attgtgaaat cggcgggccc catctttatg cgaaggatat tttaccgtca
     2581 gctccgacga agacggacca actacttgta gcgcgtactg cgtctgaaga attagaccac
     2641 tatcgcaaaa ttgctcgggt agccggagac tttggcgtcg atgtatcgtt cgtattgagc
     2701 tggccaaata agaagcgtta cctggaagca ttccggggaa ccatcaacac ctgggaggat
     2761 ttcgcggttt tcggctttct catcgatcgg gtcggacgtt accaactcga agagttcttc
     2821 ggctgcacct atcagccgct aaccgagatt ctgccgatca tcatcaaaga agaaatcggc
     2881 catgtcggtt acggcgaaag caagactgcc gagctcgcgg caaaaggaga agaatccaga
     2941 gagaaagtcc aggcagcgct ggatagatgg tacgttaaag ccctggacat gttcggacgt
     3001 tcggattcga cacgtgacga gcgctatatc tactgggggt taaaacgtag aaccaacgag
     3061 caggcgcggc aggaattcat ccgcgagctt gacccgctca ttgagaaaat agggttgaga
     3121 gtgcctgatc ctatgcaggg ccggcaatat ttttgatagg aggttaagat gtgcgggaag
     3181 tctcagttac cgcgggcact cgaatgggat tctagcgact gccagcgcgc atagcattcg
     3241 tccaattcat tcccgagcgc ctcgagtcgt tcgagagcgg aagtaattgc cgcaccgggc
     3301 tgccgataga agtcagcttg tgcagtacga agttgtaact ccgactgctc tgtctccagc
     3361 acttcaattc tggccggtaa cgcctcgagt tcccgcttct ccttgtacga cagtttacta
     3421 ttattcttgc cgggtggatc cttactcgcg gcgattgcgg ccgctggagt tcggttcggc
     3481 gttttagcct ggctcgcccg actcagttcc tgacaacgaa cccaatccgc gtagccgccg
     3541 gcgtattcaa cgaacttccc cgctccttca aagaccaatg tgctcgtgac gatattgtcc
     3601 agaaaggttc gatcgtggct tacaagtaac aatgttcctc ggtaaccggc tagcagatcc
     3661 tccagcaatt ctagtgtatc gacatcgaga tcattggtgg gctcgtcgag aaccatcatg
     3721 ttggcgggtt gagtgaagat tctggcgagc agtaatcggt tacgttcgcc gcccgacaaa
     3781 gcctttaccg gcgagtcgat ccgttgcggg gggaacagaa agtctttcag atatccgatg
     3841 acatgatgcg aacggccttg aaccgtaaca taatcggaat cgctgaggtt ttcgcgcacc
     3901 gtcttttcgg gatcgagaat acgccgatgc tgatcgaagt aggccaatcg caggcgcgtg
     3961 cccattacta cttctccgct gctcggccta atctcaccca aaatcagctt gagcagcgtg
     4021 cttttgccgg agccgttggg accgataatg ccaacccgat caccgcgtaa gatgcgcgtc
     4081 gacatctccc gaacaaccag atcattttcg taggcaaaac ttacacgccg gagatcgacc
     4141 accagttttc ctgatagagc gccggcgtcg atactaaaac tggctctact ctgagagtct
     4201 aaacgccggc tgcgctcccg gcgcatctgc tggagggcct gcacccgtcc ttcattgcgt
     4261 gtgcgccgtg ctttgacacc gcgcctgatc caggcttctt cctcggcgag ctttttgtcg
     4321 aacttcgcgg cggcgcgctc ttcgatcgcc ataagctcat cttttttccg gacgtacgca
     4381 tcgatgtcgc cgggatatga ggtcaacgtg ccgcggtcaa gctcgacgat acgcgtagcc
     4441 aggtggcgaa gaaagcttcg atcgtgagtg ataaacatca atgcgccgcg gtaatttaag
     4501 agaaacttct cgagccagat aatcgtgctg atatcgaggt ggttggtggg ctcatccagc
     4561 aagaggagat cgggctcgcc aaccagagcc cgggcgagca ttacctgacg acggacgccg
     4621 ccggaacaag cctctatcgg cttgtcggga ggcaggctca gccgggtcag cactgtctca
     4681 actttctggt tcaggttcca cccgcccaat gattcgatac gcgcgtccag gttcgctaac
     4741 gcctccagcg acggtgcttc cgccgcaccg gcctcctgcg tcgcctgacg atattcgaac
     4801 aacagccttc ctagttcgcc caaaccggaa gcaaccacct gaaagaccgt tcgttccttg
     4861 tctggcgaga cgtcttgctc taaatgcgca acccgcaaac cctcatgagc ccggacgttg
     4921 cctgcttcgg gaacagcgtc gccggtgatg acacggaata atgttgtctt tccagttccg
     4981 tttcgaccta caagacaaac ccgctcacca gcctcaatct gaaattggac atgagcgagc
     5041 aacggcaaat aaccatatga cagcgaaata tcgtcgaggc gtaataaagg cataattcgt
     5101 cgcgccagca atccgttaat tttaacaatt ccgctgacga actcaaaaag cgctaacggt
     5161 taatgcaggg aaccctccgt ggagatggcg cctaccaggc gctgtttcgc ctaagaccga
     5221 ccctacctcg atcggcaatt acggattgtg gtagttcgtt tcgttatgtt gaatggtgtt
     5281 gatgaacaag agctccaaag gacgttgaga tttggttgcc agatcgacaa gacgcttggt
     5341 agcctcgttc ctacgaaaaa aattgcgaaa accaagtttc tggtagcggc tcatctgcgg
     5401 cagaaaatca gcatagatta tggcggcatg gtcgtcgatc agattacccg gtaagctctg
     5461 cgccagtgga aagccctgtc ctgcgttatt gccgaagacg atgtaatcaa ctgactcagc
     5521 aagttccggt aacgcagcaa t
//
LOCUS       DASUVS010000148        19478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1586141, whole genome shotgun sequence.
ACCESSION   DASUVS010000148 DASUVS010000000
VERSION     DASUVS010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 19478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19478
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586141"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="VF182_08255"
     CDS             complement(<1..1107)
                     /locus_tag="VF182_08255"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX6437110.1"
                     /translation="MKIVAIDTYPLVLPVQEIYGGAAGFLEDCRSLIVRVETEDGIEG
                     WGEATQGRPGNTYETLETMEIMAQKYFAPALIKMDLENTGAVLLKLHRARHGHPIAKA
                     AIEMALYDALGKFYRLPLCRFFGGPYRREIDLVGGLGMDLGPQAIGVRAAELKREGFV
                     TFKIKIGQQNHRNDIERVRAVRQAVGDEASIRVDGNGSYSFVDARSVLNELRQFRIAD
                     AEQPLARGDLSNLAELRRAVGIPIAAQESVTCAEDALRVLEEQAADLLKIKLTHIGGF
                     QKALEVASVVGAKGLPVVVGQGSACTTILSAAEMQLHSSLKNAQPGGEMTGFMRLGEQ
                     DIFSPILVTRGKATLPTTAGLGIEVNKQRLQHLAR"
     gene            complement(1104..2471)
                     /locus_tag="VF182_08260"
     CDS             complement(1104..2471)
                     /locus_tag="VF182_08260"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6437111.1"
                     /translation="MNATQRLARFAVDLNYRSVPSEVIDRVKACILDTLAVSVYGSTK
                     PWSQTVSKLVRETGARGRSTVFGASWKAQAAQATLANGVMAHAFELDNVRQPGSGVHP
                     GATAFLPAFAMAEEKKSNGKALLSAFVAACEVVSRIGVAAGNSLERRGFHAPGLTGTF
                     GGAVAAGRLLGLNQSQMVNALGIAGSYSGGLIEFSRCREGAMVKRLHLGKAAEGGVTA
                     ALLASEGFAGPESVLEGKFGFCQTFSDSPKLPYLTHQLGTKFESLNICIKRCACHINA
                     HAPIEALQKLRGKIGFAPGDIQEIVVGGIEKLVTHHANYQPKDLMMAQYSIPFCVALS
                     LYFDPTDPESFDENKLTDKKILAMMRKIRLETDSEIERKGWDRAARVTIGLANKQRHS
                     TLVIHFKGTPRNPLSHSEVEDKARKLTHRLLSERQLDALVEAVDQLEKIDDVARIGDL
                     LRKQP"
     gene            complement(2480..3463)
                     /locus_tag="VF182_08265"
     CDS             complement(2480..3463)
                     /locus_tag="VF182_08265"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6437112.1"
                     /translation="MKCKTMFAPLLVAAIAAVFSNLAWSQEFKPNKSIEVVVHTGPGG
                     GSDLFARAIAELLQKEKLVSQRLQVVNKSGGGSAVAMSYLAEKKGETHTIGFFTGVWV
                     TNPLTTAEANVTVKDLTPVVRLVLEPAVIVVKADSPYKNMKDFVEAAKKSPNQLRQSG
                     GSVTSRDNLVRLLIQKATGAQWTFISFPSGGERLSNLLGGHVQMMVIEPQEAGEQIRA
                     GNLRVIASLTEKRLASLPDVPTMKEQGIDVPIIPQARGVLAPPAAPREVVQYWEGVFD
                     RFVKTPTWKQYVEQNQFEDGYLKGPALSKFFDDLTVQMRDVLKEAGAKVVR"
     gene            complement(3540..5051)
                     /locus_tag="VF182_08270"
     CDS             complement(3540..5051)
                     /locus_tag="VF182_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013145122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6437113.1"
                     /translation="MGALENLLLGFSVALTPINLFWCLIGVILGTVVGVLPGLGPAAT
                     IAMLLPLTLKMDHTTAIIALAGIFYGARYGGSTTSILLNIPGESSSVVTCLDGYQLAR
                     QGRAGAALGISAIASFIAGTVGILGLMLIAPPLARFALRFAPPEYFGLMVLGLAMVVF
                     LAGKSMIKALLAMLAGLWIATIGTDLFTAESRFTFGQVKLLGGVDFIAATVGIFAIAE
                     VLVNLESRGGAELFRLSKGLRNLLPTLQDLKDCRFAFLNGSLVGFFVGALPGAGATVA
                     SFLSYGIEKAFSRHPEKFGTGVIEGVAAPEGANNADAGGALVPLLTLGIPGGNTTAIL
                     LGGLILWGYKPGPLLIQEHPELFWGLVASMYIGNVLLLILNIPLVPLFAQILRLPYYV
                     LYPLILGISVVGVYSVNNSLFDVWMMGLFGLLGYLMRKLDFPAAALILGMVLGDSLER
                     ALRQSLMMSQGDITILVNRPISGTLLLIAVGLLCLPAIRKIRSWRIQAVEQEG"
     gene            complement(5072..5533)
                     /locus_tag="VF182_08275"
     CDS             complement(5072..5533)
                     /locus_tag="VF182_08275"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX6437114.1"
                     /translation="MKTTKGNILAGACLAAFGLYVISVASKLPYVSEVGPGPGFFPLW
                     LGIGLMSCAGYLMLSSCSAFTKDSPSDSSTWQGAGRALVGWCGLMVAIALFAWIGFSA
                     SFVILTVFLIVALDRRPLLPSVGVAIGLAVGFHLLFVMALNVSLPAGRWGF"
     gene            complement(5754..6146)
                     /locus_tag="VF182_08280"
     CDS             complement(5754..6146)
                     /locus_tag="VF182_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6437115.1"
                     /translation="MARIVHIALKVDDLEKATQFYEKSFGFVEVETGQVRDHTSRHLT
                     DGAIDLALIKYDNENSAEARASGLGPCIHHFAIEVDDIKQSAADLEKFGCELISDPGV
                     IPIKFRAPGGTVAEIVPKARYKKAPAES"
     gene            complement(6242..7225)
                     /locus_tag="VF182_08285"
     CDS             complement(6242..7225)
                     /locus_tag="VF182_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437116.1"
                     /translation="MNFKGPVIVLLGSVLLLFGFAADGKTAESKPINIAWTGGSNWSS
                     LPYQVAVDRKFFEKEGLLVRLITMRGTTLMLSALMADEIDYVTILPFIAGAAVRGLPV
                     KIVASGAKSSGYAIISSPEISSLKGLKGKRIAINSFGSSADFAIYTALSRSGLDPDKD
                     VTLQTISGTPDARFAALISRAVDATVVGSPFEYFAEQKGFKTLVSVKEMAELVKIPIT
                     GISTAQKKIDKQPDEIVRVLRGLRAATLFIQSEREIGIELMEKTLKLDRGAAEKFYAL
                     YREQYNPDLSVPNSVVDEWIAVGTFRSKEKEKGSVQAQRVYDWRFAERARQ"
     gene            complement(7700..8065)
                     /locus_tag="VF182_08290"
     CDS             complement(7700..8065)
                     /locus_tag="VF182_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6437117.1"
                     /translation="MAVILFVEDEAVSRRSIAHFLRLSGYEVHEAENGEAALRLLSTM
                     QFDVVISDLNLPGKLNGIDVLDALKKVTHRIEAILITGRGSEEIKRRADSLGALYLEK
                     PVQLLELETALYRKVRHCA"
     gene            8696..8965
                     /locus_tag="VF182_08295"
     CDS             8696..8965
                     /locus_tag="VF182_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437118.1"
                     /translation="MVVVIGLARADYERLLQKIPIDTPAFAVLRRCPELHRWGREEPL
                     FKCVIIECNCDEALVLLEAAKQHCERAVADIEYGLKLSRAFDLIS"
     gene            complement(8958..9344)
                     /locus_tag="VF182_08300"
     CDS             complement(8958..9344)
                     /locus_tag="VF182_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6437119.1"
                     /translation="MKILIVEDNPDMRDLLCLIVERLFFLPVRACHGREGLEKAIAEK
                     PQLILMDMRMPVMDGWEAARALRTHPDTKNIPILATTALFRPHELRTCLEAGCNDYLV
                     KPFSCVDLQIKIGEMLETSPEQRLLN"
     gene            complement(9634..10077)
                     /locus_tag="VF182_08305"
     CDS             complement(9634..10077)
                     /locus_tag="VF182_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437120.1"
                     /translation="MSSVIKKAQLVKKRRADGRIAGKEWSYPTGMVWCPMKMSGMALM
                     KCAEMQKQLGCGSIKQFKIVTITKPGDVLLHWPWLRRGRECPERADEKEVRELRLALS
                     PLKLVDKSRKDPRAYCCPACGGRKAFGARHCRRCWRLSVEETSSD"
     gene            complement(10343..11626)
                     /locus_tag="VF182_08310"
     CDS             complement(10343..11626)
                     /locus_tag="VF182_08310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6437121.1"
                     /translation="MKLPFFYGWIIVGIAMLAGFLGSGVSNVTMAVALKPITEDLGWS
                     RTLTSSAITIGSVAGGLLAPLVGPLADRLGPRILLPFGAAIVGIFVMCLSLTYEPWQF
                     YATFVPARGLAETLLTGVVPMTAVTNWFYLKRPRAIGLVALSVPLGSFALSLLYQFMI
                     AAYGWRSAFMMLGIALWVLVVLPGLFFLRRQPEDMGVLPDGALPAALKSMQADRESMP
                     PTAAAERSWTLKDAMRTRTLWLLVAAASFYSLSTGGIAFHMVAYFTDMTIQPALAAGA
                     LSVMALTGAFGSGLWGALAERFHPRTLGVTTMLLAAAAVVLLTQVRGAPAAFFFAVLF
                     GLSARGGFVLMHVLIARYFGRRSFGAITSVLEPFHKGGLGVGALLAGLAFDISGSYQI
                     VFLLFIANYLIAATLTGVAREPSSPPPEQQITANT"
     gene            12017..12916
                     /gene="uvsE"
                     /locus_tag="VF182_08315"
     CDS             12017..12916
                     /gene="uvsE"
                     /locus_tag="VF182_08315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HEX6437122.1"
                     /translation="MIRIGYACINTKLDAPNRTCRLKNATPERLLELASANVAALQPI
                     LEWNAARGIELFRITSDVIPFGSHPINTVPWWRLLKPEFERLGQFIANKRLRVSMHPG
                     QFTILNSPRPEVVANSVKELEYHTAVLDALGVDNSHKIVIHLGGIYQDKRESLGRFVD
                     NCKKLVPRIRARLVIENDERCYTVADALGASKAIGVPVVFDVFHHIWNPALEALPVRS
                     IVELAAKTWRKRDGRVKIHYSNQSPGGPPGAHSKSINVGKFLQFYDTIHDLDVDIMLE
                     VKDKQQSVLKLSQALRARQLRAA"
     gene            complement(13086..13817)
                     /locus_tag="VF182_08320"
     CDS             complement(13086..13817)
                     /locus_tag="VF182_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX6437123.1"
                     /translation="MNTANSMKNDLRVMTSGAFTAAYLELVPRLELVTKKKIVTAATS
                     IGTGETSIPHRLRRGEPVDVVIVADAVLVGFIKEGLIIAESYTPVARSAIGMAVRTGA
                     RKPDISTVDALRRTLLQANSIAYSASVSGEYLSNELLQRLGIADQVLSKCKRIEGGER
                     VGAVVARGEAEIGFQQISELLPVPGIDYVTPLPPEVQKVSVFSSGVARSTGDSNAAHA
                     IVKFLASPEAAGAITKSGLEPVGNR"
     gene            14036..15100
                     /locus_tag="VF182_08325"
     CDS             14036..15100
                     /locus_tag="VF182_08325"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6437124.1"
                     /translation="MAMIDADTHVDESEETWKKLEGTSWAKYIPVTIAMPPDEAKRAG
                     YNPTNSRCWVVEGRLQNRALRDEVNHPPRILRELQDVPGRLAHMDKMGVDVQVIFPTF
                     FIRYNTSNAEAEWALTTTYNRWLADKCAQTKGRLRWAAVLPLLQPDKAVEELRWAKAH
                     GACGIFKRGFDLDRKVTDPHFFPIYEEASALDLPLCIHTGHPLPASEWDRGFPIMYSF
                     TQLVSSGLAEKFPKLRFGLIESGASWIPYAISQLGAVKRQALRGQQTRLPRLFELEPE
                     LFQANRLFVTIDPIDDIESLLKFGTEDNLMIGTDYSHTDISANLSALDEVRSWAEEGR
                     ISNAQARKILESNSKAFYGL"
     gene            15637..16035
                     /locus_tag="VF182_08330"
     CDS             15637..16035
                     /locus_tag="VF182_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437125.1"
                     /translation="MRIRVIAISFLFSVVLFASGYVLLIHGAAATSNPDLFVFPKYQY
                     GPADKVWDEIMVGIAKAEADYVRAVLRRGGVAPEFKEQIIAFRDEAIYWDSVAKIMVY
                     HSDFDSARQAGKKAEMLLRQMTGVIAGEKT"
     gene            complement(16178..16897)
                     /locus_tag="VF182_08335"
     CDS             complement(16178..16897)
                     /locus_tag="VF182_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX6437126.1"
                     /translation="MTERKLTGKIARERIRRMETPRPPDGIIEGFRALGDASGIVSDV
                     MDELGITGVIGGSVLKPTMVGQSIVGPALTVRNIIQREHVYEAARRHVNKMAEFEAHN
                     LALSGDVLVIDGVAGISNMGGISAQTGKRQGEAGAVVFGGVRDIGHSRRIGYPVWATE
                     ITPVTGKWRIETVEINGEVVVGGVRVASGDIVLADDTGVCFIPRARAAEVLALAQQKS
                     AAEEAKCEAIDSGIAVADLPA"
     gene            17149..17919
                     /locus_tag="VF182_08340"
     CDS             17149..17919
                     /locus_tag="VF182_08340"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6437127.1"
                     /translation="MGKLRHIAFISQEPKKLSDFYQKHFGFEECKVFPSGSRMVIDPL
                     FNLAFLQSRGDQAGPVVGTHRADGAELERQPGIHHYGFLVDDLNEAVAKLPASLNRGA
                     SPQVSAGVGGAEGARPAEMRFVDPWGNNVDLSSRGFLGREEKRIPGVRLLAVQVPDLA
                     AACEFYQQQFDLDIVGWTDDGTVRLSDGTITLLLTKTQLRPKSGVQYFGIQVSDVAAL
                     KRSLQDSGFAVSEDAPGQIHFIDPEGNRVAVSQTGWTN"
     gene            complement(18156..18644)
                     /locus_tag="VF182_08345"
     CDS             complement(18156..18644)
                     /locus_tag="VF182_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437128.1"
                     /translation="MNTANARPKFKWWQIALIVPFVPLIVAIAGTALILFLVSTICLH
                     ITIWTWWCLRGHDILFVYSDSPIWRDHIEKEILPHIGERSIVLNWSQRKRWRVSVARL
                     AFHHFGGYRQFNPLAVVFRPFHRTRTFRFWQPFRDYKHGHPEALRQMESEFFGLLGVQ
                     KR"
     gene            18963..>19478
                     /locus_tag="VF182_08350"
     CDS             18963..>19478
                     /locus_tag="VF182_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6437129.1"
                     /translation="MIDLYYWTTPNGHKITIFLEEAGLPYSIKPVDISKGEQFTPEFL
                     KISPNNRIPAIIDQDPADGKGSLSVFESGAILQYLAEKTGKFLPGDVRGRSAVMQWLF
                     WQMGGLGPMAGQNGHFNNYAPEKIPYAIDRYVKETERLYGVLDERLDGRDFIAGDYSI
                     ADMACYPWIVPH"
BASE COUNT         4568 a         5364 c         5260 g         4286 t
ORIGIN      
        1 tctggccaag tgttgcaatc tttgtttgtt tacttcgata ccgagaccgg cggtcgtggg
       61 cagagttgcc ttgccgcgag tcaccaggat cggactgaag atatcttgct cgccaagacg
      121 catgaagccg gtcatctcgc cgccgggttg cgcgtttttc aatgagctgt gaagctgcat
      181 ttcagcggcg gaaagaattg tcgtgcacgc gcttccctgg ccgacgacca ccggaagccc
      241 cttcgctccg acgaccgaag ccacttcgag cgctttttga aatccgccga tatgtgtgag
      301 tttgatcttt aagaggtcgg ctgcttgctc ttccaacacc cttagcgcat cttcggcgca
      361 ggtcacgctt tcttgagccg cgatcggaat gccaacagcg cgacgcagtt ccgccaggtt
      421 actcaagtct ccgcgcgcca atggctgctc ggcatcggcg atgcgaaatt gcctcagttc
      481 attcaacaca gatcgggcgt caacgaagga atacgaccca ttgccgtcga ctcgaatgga
      541 ggcttcatca ccgaccgctt ggcgaacggc gcgaacccgt tcgatgtcat tgcgatgatt
      601 ttgctggccg atttttattt tgaaggtaac aaatccctcc cgtttaagtt ccgccgcgcg
      661 caccccgatg gcttgcggtc caagatccat tcctaatcca ccgactagat ctatctctcg
      721 acgatagggc ccgccgaaaa agcgacagag cggcagccga tagaatttac ccaatgcgtc
      781 atagagagcc atctcgatgg cggccttagc gatagggtga ccgtggcgcg cacgatgaag
      841 tttcagcagg acagctccgg tgttttccaa atccatcttg atcagcgcgg gcgcaaaata
      901 cttttgcgcc atgatctcca tcgtttctaa cgtctcataa gtattgccgg ggcgtccctg
      961 cgtggcttca ccccaacctt cgatgccatc ttccgtctca acgcgaacga tcagagagcg
     1021 gcagtcttcg aggaacccgg cggcaccgcc ataaatctcc tggactggca acaccaaagg
     1081 gtaggtgtca atggcaacga tcttcatggt tgttttcgca ggagatcacc gatgcgcgcg
     1141 acatcatcga tcttttctaa ttgatcgaca gcctctacca aagcatccag ctgccgctcc
     1201 gacaagagcc tgtgggttag ctttcttgct ttgtcttcga cttccgaatg actcagagga
     1261 ttacgcggtg tccccttaaa gtgaatgacc agcgtggagt ggcgctgctt gtttgcaagg
     1321 ccaatagtga ctcgcgccgc gcggtcccat ccttttcgtt cgatctccga gtcggtttca
     1381 agacgaatct tgcgcatcat ggcaagtatt tttttgtccg tgagcttgtt ctcgtcaaaa
     1441 gattcagggt ctgtgggatc gaaatagaga ctcagggcga cgcagaaagg gatgctgtac
     1501 tgcgccatca tcaaatcttt tggctggtag ttggcatgat gggtcaccaa cttttcgatt
     1561 ccgccgacga cgatttcctg aatgtcgccc ggtgcaaaac caattttccc gcgcagtttt
     1621 tgcaacgctt cgatgggtgc gtgagcattg atgtggcagg cgcaacgctt gatgcaaatg
     1681 ttgaggcttt cgaactttgt gcccaactga tgggtgagat aagggagttt aggtgaatca
     1741 gaaaatgtct ggcagaatcc aaactttcct tcgaggacgc tttcggggcc ggcgaatcct
     1801 tcgcttgcta gaagggcggc ggtgacgcct ccttccgctg cttttcccag gtggagccgt
     1861 ttgaccatgg cgccctcccg gcagcgggaa aattcgatta gcccaccgga atacgagccg
     1921 gcgataccca gcgcgttgac catctgggat tggttaaggc caaggagccg ccccgctgcg
     1981 actgcgccgc cgaacgtgcc ggtcaatccg ggagcatgaa aacctcttct ctccagagaa
     2041 tttcccgcgg caacgccgat tcgcgagacg acttcgcacg cggctacaaa agcgctcagc
     2101 aacgctttgc cgttactttt tttctcttcc gccatggcaa aagcaggaag aaaggccgtt
     2161 gccccgggat ggacgccgga gcctggctgg cgcacattgt cgagctcaaa agcatgggcc
     2221 atgacgccgt tggcgagtgt tgcttgcgcc gcttgtgctt tccagctcgc tccaaaaacg
     2281 gtagaacgac ctcgagcgcc cgtctctcga accagtttgc tcaccgtctg gctccacggc
     2341 ttggtcgatc cgtacactga gaccgcgagg gtatctaaaa tacaagcctt gacccgatcg
     2401 atgacctcag agggaacgga tcggtaattt aagtcgacgg caaagcgcgc caaacgttgc
     2461 gtggcattca tagattatgt taacgcacga ccttggcgcc ggcctctttg agcacatcgc
     2521 gcatctggac ggtgagatca tcgaaaaact tgctcaatgc cggtcctttg agatagccgt
     2581 cttcgaactg attttgctcg acatactgct tccaagtcgg agtcttcaca aagcgatcga
     2641 acacgccttc ccagtattga acgacctcgc gcggcgcagc cggcggagcc agcacccccc
     2701 gggcttgagg tatgattgga acatcgattc cttgctcctt catagtcggg acatccggca
     2761 gagaggctaa acgtttttcg gttaaggaag cgatgacccg caaattgccc gcgcggatct
     2821 gctcgcccgc ctcctgcggt tcgatcacca tcatctgcac atgaccgccg agcaggttcg
     2881 agagtctctc gccgccgctc ggaaaagaaa tgaaggtcca ctgcgcaccg gtagcttttt
     2941 ggatcagcag gcggacgaga ttgtcgcgcg atgtgactga accaccggat tgccgaagct
     3001 ggttcggact cttcttagcc gcttcgacga aatctttcat atttttgtag ggagagtcgg
     3061 ctttaacaac aatgactgcg ggttcgagaa cgagtcgaac gaccggcgtc aaatccttaa
     3121 cagtgacgtt ggcctcagcc gtggtcaacg gattagtcac ccagacgccg gtaaagaaac
     3181 cgatggtgtg cgtctcacct tttttttcgg cgagatagga catcgccaca gcgctgccgc
     3241 cgccggattt gttgaccacc tgcagccgct gcgagacgag tttttctttc tgtaggagtt
     3301 cggcaatggc gcgagcaaag agatccgaac cgccgccggg tcccgtatgg acgaccacct
     3361 cgattgattt gttgggctta aattcctggc tccaagcaag attggagaat accgcggcaa
     3421 ttgccgccac aagtagaggc gcaaacatcg tcttacattt catctcttac cctcccttga
     3481 gcctctagaa cgaggtcttg taaaacccga cttgcagcct gcagccggac ctatcacctt
     3541 cagccctctt gctcaaccgc ctgaatcctc caagaacgaa ttttgcggat cgcaggtaag
     3601 cacaagagtc ccactgcaat cagcagtaag gttccagaga tcggtcgatt gaccaggatc
     3661 gtgatgtctc cctgtgacat catcaaagat tggcgtagtg ctcgttcgag actatcaccc
     3721 aacaccatcc ccaagatcag ggccgcggcg gggaaatcaa gcttgcgcat aagatatcca
     3781 agcaggccga acaggcccat catccaaaca tcaaaaagac tgttgttgac gctgtaaaca
     3841 ccgaccacgg agatccccaa gatgagcgga taaagaacgt agtaagggag gcgcaagatt
     3901 tgagcgaaaa gcggcacgag aggtatattc aaaatcaata gcaagacatt gccgatgtac
     3961 atgctcgcga ccaatcccca aaaaagctct gggtgctctt ggataagaag cggtccgggc
     4021 ttgtatcccc aaagaatgag gccgccgagc aggatggctg tagtattgcc gccgggtatt
     4081 cccagggtca acaaaggaac cagtgcgcca ccggcgtccg cattgtttgc gccctcgggc
     4141 gccgctactc cttcgatcac accggtgccg aatttttccg gatgccgcga gaaggctttt
     4201 tcaatgccgt aagacaaaaa tgaggcgacc gtagcgccgg cgcctgggag agcgccaacg
     4261 aagaaaccga cgagggagcc attcaaaaaa gcaaagcggc agtccttgag atcttgcaag
     4321 gtcggcaaca ggtttctcaa tccctttgaa agtctgaaga gctcagcacc gcctctagat
     4381 tccagattga ccaagacctc ggcgatagcg aaaattccga ctgtcgcggc gataaaatcg
     4441 acgccaccca gcaatttgac ctgaccgaaa gtgaatctcg actcagcggt aaagagatcc
     4501 gtgccgatgg tcgcgatcca taggcccgcc agcattgcca gcagagcctt gatcatcgat
     4561 ttgcctgcca gaaaaaccac catcgccagg cccaaaacca tcaagccgaa gtattccggt
     4621 ggagcaaagc gcaatgcgaa gcgggccaac gggggcgcaa tcagcatcaa tcccaaaatc
     4681 ccgaccgtgc cggcgataaa tgaagcgatc gcagaaattc ctaacgccgc tcctgccctc
     4741 ccttgtcgcg ccaattgata gccatccagg caggtgacca ccgacgatga ctctcccggt
     4801 atgttgagaa ggatggaagt cgtcgaccca ccgtagcgcg cgccgtaaaa gatccccgcc
     4861 agagcgataa tcgccgtagt gtgatccatc ttgagcgtca gcggcagcag catcgcgatc
     4921 gtcgccgccg gccctaatcc cggtaacacg cctaccaccg tacccaaaat cactccgatc
     4981 aaacaccaga agagattaat cggcgtcaac gcgacggaaa agccgagcag gagattttcc
     5041 agagcaccca tactttaatg ctgtcttagg atcaaaatcc ccagcgaccg gcaggtaagg
     5101 aaacattgag tgccatgaca aagaggagat ggaatcccac ggcgagaccg atcgctactc
     5161 cgacggaggg taacaagggc cgccggtcta aagccacgat cagaaatacg gtaagaatga
     5221 cgaagctggc gctaaaaccg atccaagcaa aaagagcgat agccaccatc aacccgcacc
     5281 agcctaccaa agctctgccg gcgccttgcc aggttgagga gtcactcggg gaatcttttg
     5341 tgaaagccga gcaggatgag agcatgagat aaccggcgca actcatcaat ccgatcccga
     5401 gccacagcgg gaaaaagccg ggaccgggtc caacctcgga gacatacggc agtttggacg
     5461 cgacagagat cacatagagt ccaaaggccg ccagacaagc ccccgccagg atatttcctt
     5521 tagtcgtttt caaaggacgg cttcagcgtg ccaagatata ggcttcctta ggtgaggagc
     5581 gaatccgggc tgcattggac ctcagataga tcagccgtgg agaacatcta aaaggcgcgc
     5641 atttgcgtac cgtctcggtg acccgggatc cgaatgagca cgaagatgaa tcacgaacac
     5701 agttagcccg aatcgatcga gtgcaccgag gtatcaaggt tttcagcggg atgttaggat
     5761 tctgccggtg ctttcttata tctcgccttg ggcacgatct cagcgactgt cccgccggga
     5821 gcgcgaaact tgatggggat tactcctgga tcgctgatca attcgcagcc gaacttctcc
     5881 aagtccgcgg ccgactgctt gatatcgtcg acctcgatag caaaatgatg gatgcagggt
     5941 ccaagccctg aagcgcgcgc ctcggccgag ttttcgttgt catacttgat cagggcgaga
     6001 tcgatagcgc catcggttaa atggcgtgac gtgtgatctc tcacctggcc tgtttctact
     6061 tcgacgaagc caaaactctt ctcgtagaat tgcgtcgcct tttccagatc gtcgactttg
     6121 agcgcgatgt gaacgattcg agccatggtt cctcctcagt gatcgtccga cctctaacac
     6181 ggtcgggaga agtgattcaa gatttcactt tgtttgaacg gtgaacgttt ccttgagttg
     6241 cttactgcct agctctctca gcaaacctcc agtcataaac tcgttgagcc tggacgctgc
     6301 ctttctcctt ctctttggat ctaaaggttc cgacggcgat ccactcgtct accactgagt
     6361 tcgggacact aaggtcggga ttatactgct cgcggtaaag cgcatagaat ttttcagcag
     6421 cgcctcggtc taacttcaag gtcttctcca tcagctcgat cccgatctcg cgttcgcttt
     6481 ggataaacaa agtggccgcc cttaaacctc ttagcactcg aacaatctca tcgggctgtt
     6541 tgtcgatttt cttctgcgca gtgctgattc cggtaatcgg tatctttaca agctcggcca
     6601 tctccttgac cgagaccagc gtcttgaatc ctttctgctc ggcaaagtat tcaaacggac
     6661 tgccgaccac agtggcatcg acagcgcggc tgatcaatgc cgcaaagcga gcgtcgggag
     6721 ttccgctgat ggtttgcaac gtcacatctt tgtcgggatc caagccgctt cggctcaggg
     6781 cggtatagat ggcaaaatcg gctgagctgc cgaatgagtt gatagcgatt ctttttcctt
     6841 tgagcccctt aaggctgcta atctccgggc tggagataat cgcatagccg ctcgatttcg
     6901 cccccgaggc aactattttt accggaagcc cacgtaccgc cgcgccggca ataaaaggca
     6961 ggatcgtgac ataatcgatt tcatccgcca tcagagcgct gagcatcagg gtcgttcctc
     7021 gcatggtaat cagcctgaca agaaggcctt ccttttcgaa aaatttccta tcgacggcca
     7081 cctgataggg tagcgagctc cagttcgacc cgccagtcca agcgatattg atcggtttgc
     7141 tttctgcggt tttaccatcg gcagcaaagc caaacaaaag gagaactgag ccgagaagta
     7201 caatgacggg ccccttaaag ttcatggctg atccttctaa tcgaattctc aacagcactg
     7261 gtcaaagttt aacccgtcga gcgagcattt cgcgccgacg aatattaaat ccggaatctg
     7321 gatatccgtc actggatcaa ggtcagtact gaacgactgc ttcaaggtta tagaagagaa
     7381 ggcatttcgc agtcaatgaa gaacatcgca tcttgcgact tagactgagt cccgctcgat
     7441 gacgcggcat gtcgtaaaaa ctcaaacata gctttatcgt gcttggctcg gattccccat
     7501 acggtattga gggagcgcaa ttgtcgctga aatgacacag aggtgaggag tctgtcggca
     7561 cttccaataa ggcctctaac gatgaatgga tttaccgctg gctgatcagc aagcgattac
     7621 agtaactccg gctctgcagg cctgagcttt ggtgatgggt gggcgtaaca gaagctctga
     7681 tgcatagcag accgagtgtt tacgcgcagt gccttacttt ccggtacagc gcggtttcga
     7741 gctccagcag ctgaaccggt ttttccagat aaagagcgcc taaggagtcg gctcttcttt
     7801 taatttcttc ggagccgcgc cccgtaatca ggatcgcctc tattctgtgg gtcacttttt
     7861 tcagagcatc caatacgtcg ataccattta gcttcccggg tagattcaaa tcactaatca
     7921 ccacatcgaa ctgcatggtg gacaatagcc tgagcgctgc ctcgccgttt tccgcctcat
     7981 gaacttcgta gcccgatagc ctgaggaaat gcgcaatgct gcggcgggag acggcttcgt
     8041 cttcaacaaa gaggataaca gccatgaatg cgaccctccc gcatctccga aagtgcaatt
     8101 tccacgccct cgaggagtgc gtctaatctc aagaatgtcc ggggaggtgc ttacggcggc
     8161 ggctcttgac gtagcagagc tgaaatcggt gaagacagtt agtttcaaga tcagtacagg
     8221 tcagctggtc gagttaaata atagtttgga tcctgcgacg acggtacccg caagctgtcc
     8281 gattcattcg ccggaatttg ggatccgatc acatcgaatc gggggaagca acaagcgact
     8341 tcggcattta agtcatgaga attcgctaag gagtccaagt gaataactat gatctttatc
     8401 gttagatagc gatccattct caattcggct atttcaaaca gtctaaatcg aaacaaacgg
     8461 cgtctagagg gacgttctcc ctacctatcc tcgtcgcatt ttacgtaggg gtatcacttt
     8521 ccccggcaaa gttgaccttc gttcagtctt tatttcagtg tatttcttag aatgttaccc
     8581 ggcctgtttg ttgctgtcgc ttgtgccttg caaggagaac ggccgtgata acgtttgctg
     8641 acagaattga gccttccaga gtcctccaga catcgattag agattctgaa gccgtatggt
     8701 agttgtgatt gggctcgcgc gcgctgacta cgagcgattg ctgcaaaaga ttcccattga
     8761 tactcctgca tttgcggttc tccggcgctg cccggagctt caccgatggg gacgtgaaga
     8821 accgctgttc aaatgcgtaa tcatcgagtg taattgcgac gaggcattag tgctgttaga
     8881 agccgccaag cagcattgtg aaagagcggt agcggatatt gagtacggac ttaagttgtc
     8941 ccgagccttc gatctgatca gttgagtagc ctttgttcag ggctagtttc caacatttct
     9001 ccaattttga tctggagatc gacacagcta aatggtttga caaggtagtc gttgcagccc
     9061 gcttccaagc aggtccttaa ttcgtgagga cgaaacagcg cagttgttgc caaaatcgga
     9121 atattcttcg tgtcgggatg agtccgcagg gctctcgccg cttcccagcc gtccattacc
     9181 ggcatcctca tgtccattag gatcaactgc ggcttctctg cgatagcttt ctccaagcct
     9241 tctctaccgt gacaggcgcg aaccggaaga aagaagagcc gctcgacgat aagacatagt
     9301 agatcgcgca tatccgggtt atcttcgacg attaaaatct tcattcgcct ccaccatcct
     9361 cagtttgtca gtaatttttg tgtttttgtg ttaaggactc cgaaattgcg cggggttaat
     9421 atcacaaaca attttttttt caaggcaagc taagagtcag gggtgaggaa gtcctctcag
     9481 gcagcagctt ctctcgtcgc cggattcgat caacaaaggg gtggaagtct ctcggatggg
     9541 tgtcggcgta acggtggggg cattgaagca gtaaatgcga ctgtcaattc tgtttaaagt
     9601 gtaaagtgta aatagttgta cagcgaaact agttcagtca ctgctcgttt cttcgacgga
     9661 aagacgccag cagcgccgac agtggcgcgc gccgaaagct ttacggccgc cgcacgcggg
     9721 gcaacaatag gcgcggggat cttttctgct cttgtccacc aacttgagcg ggctcagagc
     9781 caggcgcaat tcccgtactt ctttttcatc agcccgttcg gggcactcgc gaccgcgtcg
     9841 cagccaaggc cagtgaagca agacgtcgcc cggtttagtg atggttacga ttttgaactg
     9901 cttgatgctg ccgcagccga gttgcttttg catctcggcg catttcatca gcgccatgcc
     9961 gctcattttc atggggcacc agaccatgcc cgtcgggtaa ctccactcct tcccagcaat
    10021 tcgcccgtcg gcgcgccgct ttttaactag ttgggctttt tttattacgc tagacatttt
    10081 ggacgttttt cggtctcagt atgcgtcaaa tcgtttagat cgcaagcgag aaacacgtcg
    10141 agcaatcggg caatcttctt tcgacatgat tcccgcttaa taaacttggg atcgaaaaat
    10201 cacaagagga cagcaacact acttcgtgcc tatcaagata gcgctgcgac atttagcata
    10261 ccgatggcgc atgcctcagt catgaacgac cagttaggaa atattaacgc gcgcgcttct
    10321 gaccgcaaat ctctcttttg gttcaagtat ttgccgtgat ctgctgttca ggcggaggag
    10381 aagacggctc gcgagccacg ccagtgagcg tggcggcgat cagataattt gcgataaaca
    10441 acagaaaaac aatttgatag ctaccgctga tatcgaaagc caatcctgcc agcaaagctc
    10501 cgacgcccag tccaccctta tgaaagggct ctagaacgct agtgatggcg ccgaaagagc
    10561 gccggccgaa gtaacgggca atcaaaacat gcatcaaaac aaaaccgccc cgcgcgctca
    10621 atccgaatag aacggcgaaa aaaaacgccg ccggcgcgcc gcgcacttgg gttagcaaca
    10681 ccacagcggc ggcggcaagc agcatcgttg ttacgcctag ggtgcgggga tggaatcttt
    10741 ctgccagagc cccccacaag ccgctgccga atgctccggt taaggccatg acgctcaacg
    10801 ctccggcggc taacgcgggc tgaatagtca tgtccgtaaa atacgccacc atatgaaaag
    10861 caatgccgcc ggtggaaagc gaatagaagc tggcagcggc gaccaggagc caaagcgtgc
    10921 gggtgcgcat ggcatctttc aacgtccagc tccgctcggc ggccgcagtg ggcggcatcg
    10981 attcgcggtc ggcttgcatg gatttcagag ccgccggcag ggctccgtcg ggcagcactc
    11041 ccatgtcttc gggctggcga cgcaaaaaga acagtcctgg caaaactacc aacacccaga
    11101 gagcaatgcc cagcatcata aatgcgctcc gccaaccgta ggctgcaatc ataaactggt
    11161 agagcagaga caacgcaaaa gatcccaggg gcacggacag cgcgaccaag ccgatagcgc
    11221 ggggacgctt caagtaaaac cagttggtca ccgcggtcat cggcacgaca ccggtgagca
    11281 acgtttcagc caatcctcgc gcaggcacaa aggtcgcgta aaactgccag ggctcgtagg
    11341 tcaggctaag acacatgacg aatatgccga ctatggcggc gccgaaggga agcaagatgc
    11401 gcggtcccag ccggtctgca agcgggccaa ccagcggcgc caaaaggccg cctgcgaccg
    11461 atccgatcgt gatcgcggag gaagtaagtg tgcggctcca gccgagatcc tcggtgattg
    11521 gcttgagagc caccgccatt gtgacattac tcacgcccga tcctagaaag ccggcgagca
    11581 tggcgatccc gacaatgatc cagccgtaaa agaagggtag tttcacggtt tgggtttagc
    11641 gaaggcagcg ccgagtttac cagcgggaga ggagcttacc catggcgcat ggagcatttt
    11701 cccgagcaca cagtggtgtg gccgagtgat gggcaggtcg cagcaccata gtttcgttgc
    11761 aggcaaagtg tgtgaatgaa tctttcactc tagaaatcgg acggattttc aaccgaaccg
    11821 ctccggcgtg cggcattccg gcgcgaacaa gttggttctc ttatttcatc ttttggaata
    11881 gaaaaaaaga acataatcca actaatggat gcgccatctg gagtgcttgg cagccgcctt
    11941 acatcaatcc gatcattttg ttagtttgaa tggagcccgc caaaaattcg cagtcgaaat
    12001 attcaaggag attagtttga tccgcatcgg ttacgcttgc atcaacacta agctcgacgc
    12061 gcctaaccgc acctgccggc tcaagaacgc aacgccggaa agacttctcg agctggcctc
    12121 ggccaatgtt gcagcgctcc agccaatttt ggaatggaac gcggcgcgcg gcatcgagct
    12181 cttccgcatc acgtcggacg tgatcccatt tggctcgcat ccaattaata cggtcccatg
    12241 gtggcggctc ctcaagccag aatttgagcg gctcggtcag ttcatcgcaa acaaacgttt
    12301 gcgcgtctcg atgcacccgg gacaatttac gattctcaat tccccccgtc ctgaagttgt
    12361 tgctaacagc gtcaaagaac tcgagtacca tacggcggtt ctcgatgcgc ttggagtcga
    12421 taacagccat aaaatcgtta tccacctggg cggcatctat caagataagc gggagagtct
    12481 cggtcgcttc gtcgataatt gcaaaaaact cgttccacgg atcagggcgc ggctcgtgat
    12541 cgagaacgac gagcgctgct acacagttgc cgacgcgctc ggcgcctcaa aagccatcgg
    12601 cgtgccggtg gtattcgatg tctttcatca tatttggaat ccggcgctcg aagcactgcc
    12661 agtgagatcg atcgtcgagc tcgccgccaa gacctggcgc aagcgcgacg gacgggtcaa
    12721 aatacattac tcgaaccaat cgcccggagg accaccgggg gctcattcga agtcgatcaa
    12781 cgtcggaaaa tttttgcagt tttatgacac gatccacgat ctcgatgtgg acatcatgct
    12841 cgaggtcaaa gacaaacaac agtcggtttt gaagctctca caagccctgc gcgcgcgcca
    12901 actccgtgcc gcttgatttt tttgagcgaa gttagacatc caaccgacgt gttgagtgaa
    12961 ccatccaaag gcgctgcaga gcagaaactt ggctcgccag aagttcaacc gatgccatca
    13021 accttctacg aacgattgaa cagatcgaag cgactgaacg tctggaaggg ttggaacaaa
    13081 tcgcttcagc gatttcccac tggctccagc ccgctcttgg taatggcgcc agccgcttcc
    13141 ggtgacgcga ggaacttaac gattgcgtga gccgcgtttg aatctccggt actccgcgcg
    13201 acgcctgacg aaaacaccga gactttttgc acttcgggcg gcaacggtgt cacataatcg
    13261 atgccgggta cgggcagcag ctcgctgatc tgctggaagc cgatctccgc ctcgccgcgc
    13321 gccaccacag cgccgactcg ctcgccacct tcaattcgtt tgcatttgct caacacctga
    13381 tcggctatcc cgagtcgctg aagcagttcg ttggacaaat attcgccgct tacgctggcc
    13441 gaatatgcga tggagttcgc ttgcaaaagc gtgcgtctca gcgcgtccac ggtgctgatg
    13501 tccggcttac gggctccggt ccgcactgcc ataccgatgg ctgagcgcgc gaccggagta
    13561 tagctttcgg cgatgatcag gccttccttg atgaatccaa ctaataccgc atcggctaca
    13621 atgaccacgt cgacgggttc accacgtcgt agccgatgcg ggatcgaggt ttcccccgtt
    13681 cctatggagg ttgcggcggt cacgattttc ttcttggtga cgagttcgag ccgcggaacg
    13741 agctcaagat aggctgcggt aaacgcgcct gaggtcataa cgcgtaaatc atttttcatg
    13801 ctattggccg tattcatagt cgcctcaatc tgttgacatc gttgtattcg cccgctcatc
    13861 taccagtatc cccaagccta aaagcgatca agatattggc agtgatctaa cagcatcgtt
    13921 tgctgagttg tttgaaatga cagacgatgt atttatattg gacttctaaa ccggaattaa
    13981 ggttgttcag tgcgctgaaa agacatttac taacacgaga ataggagcta acccaatggc
    14041 gatgattgat gccgataccc atgtcgacga atctgaagaa acctggaaga agttggaggg
    14101 tacgtcctgg gcgaagtata ttccggtgac gatcgcgatg ccgccggacg aagccaaacg
    14161 cgccggctac aacccgacca atagtcgttg ctgggtggtg gaaggcaggc tgcagaaccg
    14221 cgcgctccgt gatgaagtca atcatccgcc gcgaatcctg cgagagctcc aggacgtgcc
    14281 gggccggctc gctcacatgg ataagatggg cgtcgatgtg caagttatct ttccgacgtt
    14341 cttcatccgc tacaatacca gcaacgcgga agcggaatgg gcgctgacca cgacctataa
    14401 ccgctggctt gcggataaat gcgcgcaaac caagggtcgg ctacgatggg cggctgtgct
    14461 accgctgctt cagccggaca aggctgtcga ggagctccgt tgggcgaaag cgcacggcgc
    14521 ctgcggcatc ttcaagcgcg gcttcgatct cgacaggaag gtaaccgatc cgcatttttt
    14581 tccaatttac gaggaagcga gcgcgctcga cctgccgctg tgcatccaca ccggacatcc
    14641 gcttccggca agcgagtggg accggggttt tccgatcatg tattcgttca cacagctggt
    14701 gagctccggg ctcgcggaga agtttcctaa acttcggttt ggcttgatcg aatcgggggc
    14761 gtcgtggatt ccctatgcca tctctcagtt gggagcggtc aaacgccagg cgctgcgcgg
    14821 ccagcagact cgattgccgc gcctgttcga actggaaccg gaattgttcc aggccaaccg
    14881 tttgttcgtt accatcgatc cgattgacga tatcgaatct ttactgaaat tcggcactga
    14941 agataatcta atgatcggca ccgactacag ccacaccgac atctccgcca atctgagcgc
    15001 tttggacgaa gtccgcagct gggctgaaga gggcaggatc agcaatgctc aagcaaggaa
    15061 aattctcgag agcaattcga aggcgttcta cggcctgtag aatacagatg catcgcggtg
    15121 cttgaggatt ctcatcggga tcgcttcctt cccggcgccc ttccggcata cgaccggcag
    15181 atgttgaacc gtgaatggat agggccccca caacccgcgc tggatctggc cgctccaatc
    15241 ttaagttcgg ccaaatcgct ttccggtttg aggccgccag ttctctgtgt tccaggaact
    15301 aatctctact gcaaacctga tgattgccgg cagctaaagc cattcgactg cctcgctaac
    15361 cctgagcggc ggtaagaagt ctgcaacccg tacaagtgaa atagaatcgc gaacccagtt
    15421 aaaaatcgct cgacattaac ttcaacttgt tctatggccc gggaagagag atctgcactt
    15481 cggatgccat accaggcgag tagcggaacg cctggagccg atacgagttg caaacgacac
    15541 ccgtagcgcc gccgccgaaa atgcgacatt ctggaaaggg atgcaggcga gagagaaggg
    15601 aaagcacgtg tatataaagg aagagaggag actaaaatga gaattagagt gattgctata
    15661 tcctttctct tctctgtggt tctttttgcc tccggctatg tgctgctgat tcacggcgct
    15721 gcggccacgt ccaaccctga tctgttcgtt ttccccaaat atcagtacgg ccccgcggac
    15781 aaagtttggg acgaaattat ggtcggaatc gccaaggcag aggctgacta cgtgcgcgcg
    15841 gttttgcgtc gcgggggtgt cgccccggaa ttcaaggagc agatcatcgc gtttcgtgac
    15901 gaggccattt actgggattc tgtcgctaag atcatggtct atcattccga ctttgattct
    15961 gcccgtcagg ccgggaagaa agctgaaatg ctgctccggc aaatgacggg cgtgatagcg
    16021 ggagaaaaga cttagctgag tgcgcgcgca gcggaatggc gacgtttccg ccagaaacta
    16081 gcaggctgct gaaaaaaatc catatgcttc gttgccgctc aatcgcctca cctctggatc
    16141 ttttgacagc ctgcaagagg cttttttcaa cagcctgcta ggccggcaaa tcggcaacgg
    16201 caatacccga atcgatagct tcacacttgg cttcttcggc ggcggatttt tgttgcgcca
    16261 gcgcgagcac ctcggcggcg cgggcgcgag gaataaagca aacgccggtg tcatcggcga
    16321 gcacgatatc tccggacgcc acgcgcaccc cgccgaccac gacctcgccg ttgatctcga
    16381 cggtttcaat gcgccactta ccggtgaccg gcgtgatctc cgtcgcccat accggatagc
    16441 cgatccggcg cgagtggccg atgtcgcgca cgccgccgaa tacgacagcg cccgcctcac
    16501 cctggcgttt gccggtttgg gcggagatgc cgcccatgtt ggagattccc gccacgccat
    16561 cgatcaccag gacgtcgccg gatagagcga gattgtgcgc ttcgaactcg gccatcttat
    16621 tgacatgccg gcgcgcggct tcgtagacat gctcgcgttg gatgatgttg cgaaccgtga
    16681 gggccggacc aacaatcgac tgccccacca tcgttggttt gagcactgat ccgccgatga
    16741 cgccggtaat gccaagctca tccatcacgt cggagacgat gccgctggca tcgccgagcg
    16801 cccggaagcc ttcgatgatt ccgtccggcg gtcgtggcgt ttccatcctt cgaatgcgct
    16861 cacgcgcgat cttaccggta agttttcgtt ccgtcatcat cacctcccgg agcatgtatc
    16921 gcggccaaaa taggacgggt ggaatttacc gtcaagccgg ccagcaagtt tttttattgt
    16981 gcgcatgtag aagcagcttg cgctgcgcgt ccaatgctac cgaacgaacg agatctcggt
    17041 gggcttgaat ttcatgtcac caatgtgggc gcgggtgcat aagaccatgc gcgttgacag
    17101 ccgataaatc cccggcctat aaacaagtga ccaatgcgga ggtgaaacat gggtaagctc
    17161 aggcacatcg catttatttc tcaggagcca aagaaactca gcgattttta ccaaaagcat
    17221 tttggcttcg aggaatgcaa agtgtttccc agcggctcgc gcatggtgat cgatcccctt
    17281 tttaatttgg cgtttctaca gagcaggggt gaccaagcgg ggccggtggt cggcacccac
    17341 cgcgccgatg gcgcggagct ggagcggcag ccgggaattc atcattacgg ttttcttgtc
    17401 gatgacttga acgaggccgt cgccaagctg ccggcatcct tgaatcgcgg tgcgagcccg
    17461 caagtttcgg cgggagtcgg tggtgccgag ggggcgcggc cggcggaaat gcgatttgtc
    17521 gatccctggg gaaacaatgt cgatctttcg tcgcgcggct ttctcggcag ggaagagaaa
    17581 aggatccccg gagtgcgact gctcgcggtg caagtccccg acttggccgc ggcgtgcgag
    17641 ttttatcaac agcagttcga tctcgacatc gtcggttgga ccgatgatgg gacggtgcgc
    17701 ctgagtgacg gaaccataac cttgctcttg accaaaacac agctgcgtcc caagagcggc
    17761 gtgcaatact tcggtatcca ggtaagcgac gtggcggcac tcaagaggag cttgcaagac
    17821 agcggcttcg cggtttcaga ggacgcgccc gggcagattc acttcatcga tccggaaggg
    17881 aatcgggtgg ccgtgtcgca gaccggctgg acgaactaaa ctccgcactt caaggtccct
    17941 accgtagtcc gagctctttc tggatctctc gcaaaatggc gaagtcggca acttcgccga
    18001 gcggaatggt catcggctaa caactaaaac gatattgaga gagcgcggct ggcgcagtac
    18061 cgttcgtcac cccgtagacg acagctgcga tctcaccgcc gttttctcac ccgaaagatg
    18121 cgagcgacag tccggcgtat tgtcagtccg acggctcatc tcttttggac tccaagcaac
    18181 ccaaagaact cactctccat ctgacgaagg gcttctggat gcccgtgctt gtaatctcta
    18241 aagggttgcc aaaagcgaaa cgttcgcgtg cggtggaagg gacggaagac gaccgcgagc
    18301 ggattgaatt gccgataacc cccgaaatga tggaacgcca gccgagcaac cgaaacacgc
    18361 cagcgcttac gctgtgacca attgagaacg atgctccgct caccgatgtg cggaagaatc
    18421 tctttttcga tgtggtctcg ccaaatggga ctgtcggagt agacgaagag gatgtcatgc
    18481 ccacgaagac accaccacgt ccagatggta atgtgcaggc aaatcgttga taccaggaag
    18541 agaataagtg cggtgcctgc aatagcgacg attagcggaa cgaacggaac gatcagcgca
    18601 atctgccacc atttgaactt tggtctagcg ttcgcagtgt tcatcagccg ctgataagcc
    18661 tacgccatca cgcgattgtg aatgatccgg atgatgcccg caatatgatg atcttgcgcc
    18721 catcgagtgt cgggaaaata tgttgaactc cgctcgctgt cggagagatg taccttctcg
    18781 atccgggggt tgtcaatcat tgatcgacga cgtctactct agcccgtcta ttctttcgat
    18841 ctcgcgccgt gtgaggctct aaatgatccg acttgaacgc ggaagaaggc tggtaagctg
    18901 atcttattga ccctttccgc acgggcggtg tagtttgaca aaaacgacgg gggtaaatca
    18961 cgatgatcga tctctactat tggacgacgc cgaacggaca caaaatcaca atcttcctgg
    19021 aggaagccgg gctgccgtac tcgatcaagc cagttgatat ttctaaaggc gagcaattca
    19081 cgccggagtt tctgaaaatc tcgccgaaca atcgcatacc cgcgataatc gaccaagacc
    19141 cggccgacgg caaaggatcg ctcagcgtgt ttgagtccgg cgcaatcctt caatatctgg
    19201 ccgagaagac cggtaaattt ttgccgggcg atgtgcgcgg gcggagcgca gtgatgcagt
    19261 ggctgttctg gcagatgggc gggctcggtc ccatggcggg gcaaaacggc catttcaaca
    19321 actatgcgcc ggagaagata ccttacgcga tcgaccgtta cgtcaaggaa accgaacggc
    19381 tttacggcgt cctcgacgag cggctcgacg gccgcgactt tatcgcgggc gattattcga
    19441 ttgccgatat ggcctgctat ccctggatcg tgccgcat
//
LOCUS       DASUVS010000149         4769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_56166, whole genome shotgun sequence.
ACCESSION   DASUVS010000149 DASUVS010000000
VERSION     DASUVS010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56166"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /gene="rnc"
                     /locus_tag="VF182_08355"
     CDS             complement(<1..625)
                     /gene="rnc"
                     /locus_tag="VF182_08355"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX6437130.1"
                     /translation="MKNPSQLEALQKELGYTFKDRSLLVKALTHVSHDRKKTGSNNEV
                     LEFLGDAVLDLAISDLLIRRFPERSEGDLSRMRAGLVNAVALAEKGSALALGPLLLLG
                     KGEERSAGRKKQSILAGAFEALLGGVYQDGGYDPARRIIERYFAADIIERRLGQQDYK
                     TRLQEISQLLFRAPPVYHLVSESGPDHAKYFVTEISVGGNVLGIGEGK"
     gene            complement(634..2001)
                     /gene="mtaB"
                     /locus_tag="VF182_08360"
     CDS             complement(634..2001)
                     /gene="mtaB"
                     /locus_tag="VF182_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEX6437131.1"
                     /translation="MPRAIAQHMRIALTTLGCKINQYDSAVIQSRLEEKHTFVPFDEP
                     ADCYIINTCTVTDRADWEARQLVRRAKRRSPNSKVLLTGCYAQVSPAELARVQGVDFI
                     VGLNRLDDLLRLVETTDFNSPQIHVSDVKRQRGVPVLGTRALPGHTRAFLKIQEGCNY
                     TCTYCIIPTARGLSRSVAPREVLNQVRLLADSGYAEIVLTGIHLGGYGQDLSPRIDLT
                     ALLEMLVDSTLVRRIRLSSLDPREVPDRLLGLIAGSEAICPHLHICAQAGEDTILKQM
                     RRNYDTAYYRELLNKIRERLPEAALGSDIIVGFPGESDEQFESSLAYFASLPLTYFHV
                     FPYSQRRGTAASIFSHPVPISVKKLRAQRMRQLGAEKKEHFCQTFRGRRMAVLIEEKI
                     DRGSGGRRGFTRNYLPIIVNRGNYRVNEEVDVQIDGFAGGCLRGAAIYPGEPGERENP
                     SEASA"
     gene            complement(2028..3104)
                     /gene="mnmA"
                     /locus_tag="VF182_08365"
     CDS             complement(2028..3104)
                     /gene="mnmA"
                     /locus_tag="VF182_08365"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX6437132.1"
                     /translation="MTKRSPKVVVAMSGGVDSSVTAGLLVEQGYDVVGVSLRMWEGNL
                     GPRVCSDHRGAEEVAEFLGIPHELLDLRSQFGETVVKPFAQDYIGGRTPNPCVACNRD
                     FKFGSLLQWAKQQHADYVATGHYARVARDNPSGQASLLRGIDQAKDQSYFLFALSQDQ
                     LAHTIFPVGDLRKNQVREIARGFGLSVADRPESQDICFGDYKTLVQAHADAKDLGTGD
                     IVNRSGKVLGRHNGIHALTIGQRRGLGIAAPEASYVLHIDRASKRVVIGTRSELACSG
                     LIAERMNWVEPPVGEEFAAQVQIRYRSPAIPCVIRIAENNACEVHFKETYLGVTPGQA
                     AVVYRGEKLLGGGWIEHAIRSERD"
     gene            complement(3235..3966)
                     /gene="cysE"
                     /locus_tag="VF182_08370"
     CDS             complement(3235..3966)
                     /gene="cysE"
                     /locus_tag="VF182_08370"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003365098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX6437133.1"
                     /translation="MFDVVKEDIRVVFDRDPAARNVWEIIFTYPGFHALFFYRVAHAT
                     WINGFKGLARFASNLGRFFTGIEIHPGAQIGRRFFIDHGMGVVIGETTEIKDDVTIYQ
                     GVTLGGTSLRKEKRHPTVEDWVVIGAGATVLGPVTIGHNSRIGSGSVVVSSVPPHSTV
                     VGIPGRVVEGEAARRDAHAHIIDLDHARLPDPIAKAIGALADYIQKLEKRVDELTDRQ
                     GGLEEQRAEQSEVIDKVKELLKNSG"
     gene            complement(4020..>4769)
                     /locus_tag="VF182_08375"
     CDS             complement(4020..>4769)
                     /locus_tag="VF182_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEX6437134.1"
                     /translation="LANIQPNKRQPYVGDSAFAHKGGLHVSGVLKNRETYEHIDPDFV
                     GNRQRVLVSDLAGRSNVVYKGKEYGIDLEGQDDAVREILRRTKELESQGYEFEAAEAS
                     FELLIQQALGRKRKNFKLVGFRVIDEKRQEGEVPLSEATIMVEVDGMMEHAAAMGNGP
                     VNALDQALRRALTKFYPGLKDVELLDYKVRVLSSGEGTAASVRVLIESGDGKARWGTV
                     GVSHNVIEASWQALVDSIDYKLYKDGRRRAN"
BASE COUNT         1037 a         1431 c         1243 g         1058 t
ORIGIN      
        1 atttgccttc gccgataccc agcacgttac caccgacgga gatctccgtg acgaaatatt
       61 tggcgtgatc gggaccactt tcagaaacca aatgataaac cggcggcgct cgaaaaagca
      121 gttggctaat ttcttggagt cgggttttat aatcctgctg gcccagtctt ctttcgataa
      181 tatcagcggc gaaatagcgt tcgatgatgc gccgagccgg atcgtatcca ccatcttgat
      241 aaactccgcc cagtaaggct tcgaacgcgc ccgccagaat cgactgcttt tttcttccgg
      301 cactgcgctc ttcgccttta cccagaagca acagcgggcc gagcgccaat gcgctcccct
      361 tctcggccag cgcgacagcg ttgaccagtc cagcgcgcat gcgcgataag tctccctcgc
      421 tccgctcggg aaaacgccga atcaacaaat cactgatcgc tagatcgagc accgcgtcgc
      481 cgagaaactc aaggacttcg ttattgcttc cggttttttt tcgatcgtga gagacgtgcg
      541 tcagagcctt aaccaagagc gaccggtctt tgaaggtata accgagctct ttttgaagag
      601 cctctaactg tgatggattt ttcaaagtcc ctgctaggcg ctcgcctcgc ttggattttc
      661 gcgctcgcca ggctcgccag gatagatggc tgcgcccctc aagcagcccc ctgcgaagcc
      721 gtcgatctgg acatccacct cttcattcac ccggtaattt ccacggttga cgatgatggg
      781 caagtaattt ctggtaaagc cgcggcgccc gccgctgccg cggtcgatct tctcttcgat
      841 taaaaccgcc atgcgccggc cgcggaaggt ttggcaaaag tgttcttttt tctcggcgcc
      901 gagttgtcgc atcctttgcg cgcgcaattt cttgaccgag ataggcaccg ggtgcgagaa
      961 gatcgaggcg gcagtgccgc gacgctgcga atacggaaag acgtgaaaat aagtcagcgg
     1021 cagcgatgcg aagtaagcca atgagctttc gaactgctca tcgctctcac ccggaaagcc
     1081 gacgatgata tcgctgccaa gagcggcttc cggcaatcgt tcacgaattt tatttaacag
     1141 ctcgcgataa taggcggtat cgtaattgcg cctcatctgt ttgagaatcg tgtcctcacc
     1201 cgcctgcgcg cagatatgca ggtgcgggca gatagcctct gagccggcga tgagaccgag
     1261 caatcggtcc ggcacctcgc gaggatcgag agagctcagg cgaattcgtc gaaccagggt
     1321 gctgtcgacg agcatttcca acagagcggt gagatcgatc ctcgggctta gatcttgtcc
     1381 gtatccgccg agatgaattc cggtcaggac gatctctgca taacccgagt cggctagcaa
     1441 cctaacttga ttcagaactt cgcgcggcgc gacactgcga ctcaggccgc gggcggtcgg
     1501 gataatgcag taggtacagg tataattaca gccttcttga atcttgagga atgcgcgagt
     1561 atgtccgggc aaagcccggg tgccgagcac cggcaccccg cgctgccgtt tcacatcgct
     1621 gacgtgaatt tggggagaat tgaaatctgt cgtttcgacc aggcgcagca aatcatcgag
     1681 gcggttaagc ccgacgataa agtcgacacc ttgaactcgc gccagctctg ccggacttac
     1741 ctgcgcgtaa cagccggtca ggagtacctt gctgttcggg ctccggcgct tagcgcggcg
     1801 cactagctgc cgtgcctccc aatcggcgcg atcggtcact gtgcaggtat tgatgatgta
     1861 gcaatcggct ggttcgtcga agggcacaaa cgtgtgcttc tcttccaagc gcgactgaat
     1921 taccgccgaa tcatactggt tgattttaca accgagcgtc gtcagtgcta tccgcatatg
     1981 ctgggcaatg gcacgaggca agagatgata gtctctaatg cctaatgcta atctctttct
     2041 gatcgaatcg cgtgttcgat ccagcctccg ccaaggagct tctcgccgcg ataaacgacc
     2101 gccgcctgac ccggcgttac ccccaggtag gtttctttga agtgcacctc gcaagcgttg
     2161 ttttcggcta tacgtatgac acatggaatc gccggcgaac ggtaacggat ttgcacctgc
     2221 gccgcgaact cttcgccgac cggcggctcg acccaattca tccgctccgc aatcagaccg
     2281 ctgcaggcaa gttcactcct agtcccaatg acgacccgct tcgacgctcg atcaatatgg
     2341 agaacataag atgcttctgg tgcggcaatc ccaagccccc gacgttggcc gatagtgaga
     2401 gcgtgaatgc cgttgtgcct gcccaatact ttaccggatc tgttgacgat atcgccggtg
     2461 ccaagatcct tggcatccgc atgggcctgg accaaagtct tgtagtcacc gaagcagatg
     2521 tcctggctct cggggcgatc ggccaccgat aaaccgaacc cgcgcgctat ttcccggacc
     2581 tggtttttac gcaaatcgcc aacgggaaaa atcgtgtgcg ccaattgatc ctgcgaaaga
     2641 gcaaacaaga aatacgactg atcttttgcc tgatcgatgc cgcgcagcag tgaagcttgc
     2701 ccggaagggt tgtcgcgcgc cacccgtgcg tagtggccgg tggccacgta gtccgcatgt
     2761 tgctgtttgg cccattgcag caaggacccg aacttgaaat cacgattgca ggcgacgcat
     2821 ggattgggcg tgcgcccacc aatatagtcc tgagcgaacg gtttaacgac ggtttcgccg
     2881 aattgtgaac gcaaatccaa aagctcgtga ggaatgccga gaaactcggc gacctcttcg
     2941 gcaccgcgat ggtcagaaca gacgcgcggg cccaggtttc cttcccacat ccgcagcgat
     3001 acgcccacca cgtcgtaacc ctgctcgacg agcaagcccg ccgtgaccga actgtcgaca
     3061 ccgccgctca tggccacgac aacctttgga cttctcttag tcataagact ctgaataatt
     3121 tatttcttgc ccgtgaattg aaccgcagcg gagacgcaat cgcgcaattt taaaatcgtc
     3181 attcatctcg tcgcgaaatg cgtatttcgt gcaacagctt gcgctctcgc caattcaacc
     3241 cgaatttttg agcaactctt taactttatc gatgacttcg ctctgctcgg ctcgctgctc
     3301 ctcgagcccg ccttgccggt cggtcagctc gtcgacccgt ttctccagct tctgaatgta
     3361 atctgccagc gcgccaatag ctttcgcgat ggggtcaggc aatcgggcgt gatccagatc
     3421 gatgatatga gcgtgagcgt cccggcgggc ggcttcgcct tcaactaccc gtcccggaat
     3481 tccgacaaca gtcgaatgcg gcgggacact ggaaaccaca accgatcctg aaccgattct
     3541 actgttgtga ccgatcgtca ccggcccaag caccgtcgcc ccggcgccta tgacaaccca
     3601 atcctcgacg gtcgggtggc gcttttcttt tctcaagctg gtgccgccga gggtgacccc
     3661 ttgatagatc gtcacatcat ctttgatttc ggtggtctcg ccgatcacca cgcccatacc
     3721 gtgatcgatg aaaaagcgcc ggccgatttg ggctcccgga tgaatttcaa tgccggtaaa
     3781 gaaacggccg agattgctgg cgaatcgagc taagccttta aagccgttga tccatgtcgc
     3841 gtgggccacc cgatagaaga agagcgcgtg aaatccggga taggtaaaaa taatctccca
     3901 gacattacgg gctgccggat cgcgatcgaa aacgacccga atgtcttctt tgaccacatc
     3961 aaacataagg ttggcttcat caggcccaga gcagcccctg acagtaacga ccactctgct
     4021 taattggctc ggcgccggcc gtccttataa agcttgtagt cgatgctgtc caccagagcc
     4081 tgccaactcg cctctatcac gttgtgcgag acaccaacgg taccccaacg cgctttgccg
     4141 tcgcccgatt cgatcagaac tcgcaccgaa gctgcggttc cctccccgga ggatagcacc
     4201 cggactttat aatccaacaa ctcaacatct ttaagaccgg gataaaactt tgtcagagcc
     4261 cggcgcagag cctgatcgag agcgttaacc gggccgttgc ccatggccgc ggcatgctcc
     4321 atcatgccat cgacttcgac catgatcgtc gcttcggaaa gcggcacttc gccttcctgc
     4381 ctcttttcat cgatgacgcg aaagccgacc agtttgaaat tttttctctt tctacccagc
     4441 gcttgctgga tcagcagctc gaatgaggcc tccgcggctt cgaactcgta gccctggctc
     4501 tccagttcct tggtgcggcg caaaatctcc cgcaccgcgt catcctggcc ctcgagatcg
     4561 atgccgtact ctttaccctt gtaaacaacg ttgctgcgcc ccgcgagatc cgaaaccagc
     4621 acccgctgcc gattaccgac gaaatccgga tcgatatgct cgtaggtctc gcggttcttc
     4681 agtaccccgg acacatgcag tccgccttta tgcgcaaagg cactgtcgcc tacgtaaggc
     4741 tgccgcttat tcggttgaat attcgcgag
//
LOCUS       DASUVS010000150         9002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1804699, whole genome shotgun sequence.
ACCESSION   DASUVS010000150 DASUVS010000000
VERSION     DASUVS010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9002
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804699"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VF182_08380"
     CDS             complement(<1..211)
                     /locus_tag="VF182_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437135.1"
                     /translation="MALVDDLAARRASHFLALILDNEVTVGEFVTEPPLRWTRCIQRN
                     GLFQVAQAYPTLLTEAQAKVEMRNWD"
     gene            427..660
                     /locus_tag="VF182_08385"
     CDS             427..660
                     /locus_tag="VF182_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017866784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437136.1"
                     /translation="MKNTIYAILVTGALMQAGCSPEFWGGTALGALGAGGGYEYNNKR
                     QMDKLEDDYRAGRMNREEYQDRKQQIERGSIIY"
     gene            complement(742..1152)
                     /locus_tag="VF182_08390"
     CDS             complement(742..1152)
                     /locus_tag="VF182_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437137.1"
                     /translation="MSIGQRRIYISYSLGRRALHNRHHLWLLRQTLAHEIAHEIARHA
                     DYRQINFNRSSNEYGMTSRDVGLPWYVIFQPYSLEKELEADLDGIEYWKSLQWDCTIW
                     VQILADFERQNYYGDKNHPTQLRLEQASAACRSE"
     gene            1671..2552
                     /locus_tag="VF182_08395"
     CDS             1671..2552
                     /locus_tag="VF182_08395"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX6437138.1"
                     /translation="MRRSLFFILALICGGLAVFALSLLVFNSESLLLRHLERTLGRKI
                     LAREVQITFIPALGLHLKEFSMADDPIFSSGAFLTADALQVRFKFLPLFLYQLRIKEI
                     ILRDPVINVIRNHRGIYNFSSIGLEKPRKTIIEQPVKSQAQAKEYAPALIPSSSIQVI
                     NGSLRYLDQTNGTDMIVSRLDLKVVDLQDRNSFAVELAMAVFAMEQNFQLKAAVGPLV
                     PQRSFRDLPLDGELRFDRFDMGKLRAVLPVIKKGLPKALDLRGVYSTQGVRFKGTLNR
                     PSLKGQVEGTDASFRFD"
     gene            complement(2553..3182)
                     /locus_tag="VF182_08400"
     CDS             complement(2553..3182)
                     /locus_tag="VF182_08400"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX6437139.1"
                     /translation="MKLILASTSPRRREILALLGLPFDVIQPDFEEMLSFHRSIEEEV
                     LEFATGKAKSVAGRHPEAIVIGSDTMISLDHEKIGKPRDLTDARAMLNKLAGRTHCIY
                     TGVGIIDGSGGPGLTALERVTVKMRDMSDTKIQDYLACGESLDKAGSYSIQDKGRELI
                     ASIEGDYLAAVGLPLKSIADYLKERGIAVPLDVDTLYENKFVLNWESFR"
     gene            complement(3200..4417)
                     /locus_tag="VF182_08405"
     CDS             complement(3200..4417)
                     /locus_tag="VF182_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEX6437140.1"
                     /translation="MSPTRSLESIPETNFWRLINRPIVGLSPMDGVTDASFRYITAKH
                     GGPDVIFTEFVNIQAAFHAAHTLIKDLTYCEIERPIVAQIYGRSPELFYKVAHIVCEL
                     GFDGLDINMGCPARKVAAAGCGAALIREPGLAITIIRAVKVGIKDWCAGQTLSDIEVE
                     PELIKKIKMANRLRTGTDIALERQPIPVSVKTRLGYDRIVVDDWIATLLEENPAAISL
                     HGRTLQQGYKGDADWGAIARAVKVGEDSETLILGNGDLQDMAQVQRRLRETEVDGVLL
                     GRAAQGNPWIFRAKHQLKHALAEKRQLCIASMPVSLEERFRVISEHSQHHAQQASDEN
                     FVGIRKHLTWYCRGFRGAAEMRSQMVRVKTAGEVEQCLKNFIAQSAEAERRSNDRHEG
                     PAAVVTPDRHVIL"
     gene            complement(4456..4599)
                     /locus_tag="VF182_08410"
     CDS             complement(4456..4599)
                     /locus_tag="VF182_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437141.1"
                     /translation="MKALALVDRIHLHFNLPVTTVELPIDDALGMMLSPGASSLGLGA
                     HMD"
     gene            4720..7185
                     /locus_tag="VF182_08415"
     CDS             4720..7185
                     /locus_tag="VF182_08415"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX6437142.1"
                     /translation="MRKWAFILGSILLVIVIAAVVAVLNINSYIQRNREYLLQQAEKA
                     VGRQIEVQDIEVNLWNGIGIRFENFSLADDPAFSSNPFVQAKSLQANVRLLPLLRKDV
                     QVKRLILHEPGINIIRNKQGVYNFASLGKPDKKKDKETEGARDKSARERAALLISLVE
                     ISGGGARYRDLADGTDLAIQQLDLRAQDLNFDRPVSINLSAAVLASKQNVKLRLLIGP
                     ISPESDYRNMALDSQLDIDPLDWSQLRKAVPKLSAALPKNLELGGIYKLTGLKLKGTL
                     QDFALNGSIDGTQGLIQYADYFHKPPGIPLVFESAAHYSNDKITIQNGGLRLHTLASR
                     FKGDVVLGNKPVVALFVDADPASLEGWEKLIPVVVDYDIKGKMGLRATVRGPVGAGKT
                     PQVDGNLSLQSASIKIPDLPKAIDNIDAQVKFSGARAEIKETNLNLGTSHIRLAAVIE
                     KFSPLTLSYKLSSAELALADMQPSLPAERSKDVLRDLSSDGQVSTADGQTIVTGKIIS
                     GAGRLYNMDYKSLEANLSSAKNSALVRSFRANLLNGSVQLKGDYRFDERPRFSTASEI
                     KGIDLVQLYQYFTQEQPDMRGSLNGNLKLSGQGDQWQEIKPSLHGAGQTEVLNGTLLN
                     FNIADAVLSGATGIPGLTNLINPAVRKKYPETFDAKDTVFKELQTQIDIGESRVNLKD
                     LRIAAAQFRVDGNGWVDFERRVDFRGVVRFSPALSGDIAGAVREIRLLFNKNNELEIP
                     FTLSGQMPNVRPRPDASFLTKSLQRGLLQKGAEELQEQLFGPRERRAPNDRQPDQERQ
                     QKPSSPEDLIRRGLEGLFGRQKR"
     gene            complement(7203..8480)
                     /locus_tag="VF182_08420"
     CDS             complement(7203..8480)
                     /locus_tag="VF182_08420"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6437143.1"
                     /translation="MLVFNGIKLFSLCVLTSWPMNTSTARPLAALYSATFLSGAWAMV
                     IPTIPVIAREFDVSAGGAAQIVTAFAIGKFVGTVIAGIVLDRMGTRVGLIAGPLVASV
                     ASFATVIAPWLSAILVLVLVMGAADSLWSSAREIAGIDLADRKQRGRVMSSLHGTYNL
                     GAAVCPLLGGWLTDMFSYKAAFIGYAVATALAVGLGFVSRGTTGPALAPSKVPPNNTW
                     RFSRVREVFHGAGLLLKEIRPDLRSTYLALVLATVASQSQRILVQSILPLYAGFYLQL
                     SPTQIGMLFSISGLIVFAMIIPAGLVMDRVGRKWCTVPSTGIPALVFVLIPFTTSFTQ
                     LSILVGLSGLAQGLSLGSIATSTYDVVPSHARGRLQAVRRTVAELGSGLAPVIGGYLA
                     NAYNPGLPFLVYAPLLLFSAVLLGIVGKETLDR"
BASE COUNT         2228 a         2387 c         2316 g         2071 t
ORIGIN      
        1 catcccagtt tctcatttcc actttggctt gcgcttcggt cagtagagtt ggataagctt
       61 gcgccacttg aaacaatcca ttgcgctgaa tgcagcgggt ccaacgaagc ggcggttcgg
      121 tgacaaattc tcccaccgta acctcattgt ccaagatgag cgcaagaaag tggctggctc
      181 tgcgtgccgc aaggtcatca actaacgcca taacatatta ctcctcattc agtatgactg
      241 catgctctcc cctacacaac agcataatcg ctgtgcgaag tgtgtgtact aaacgtgaga
      301 acggaattaa acagtgcaac acaaatgcga catccgcaat gcgccacagc gaaatttcga
      361 taagccgcca gcggcatagg aattgccttt aacgataggc aacaaaatga tgaaaggagt
      421 ctagttatga agaacaccat ttacgccata ttagtaaccg gcgcattaat gcaggcgggt
      481 tgtagcccgg agttttgggg gggaacagca ctcggtgctc tgggcgccgg cggaggctac
      541 gaatacaata acaaaaggca gatggataag ctcgaagacg attatcgggc tggtcgcatg
      601 aaccgggaag agtaccagga tcgtaagcag caaatcgaac gcggatcaat aatctattaa
      661 acgaagtcgc gattgatcta gctttcggaa agcatcacgg ccgccgttcg gaagagttac
      721 acatgactgg gtgccgctga ctcactccga acggcacgcc gccgatgcct gctctaggcg
      781 caattgtgtc ggatgattct tgtcgccata gtagttttgg cgttcgaagt ccgctaaaat
      841 ctgcacccag atggtgcagt cccactgcaa gcttttccaa tactcgatcc cgtcaaggtc
      901 cgcctccagt tccttctcca acgagtatgg ctgaaagatg acataccacg gtaacccaac
      961 atcacgactg gtcattccgt attcgttcga cgagcgattg aaattgatct gtcgatagtc
     1021 cgcatgacgc gcaatctcat gtgctatctc gtgcgccaag gtttgcctga gaagccagag
     1081 gtgatgacga ttgtgaagcg cgcgtctgcc caggctgtaa ctgatataaa tccggcgttg
     1141 cccgatgctc atgccgagaa tatctgcgcg gggaaaatca gagagcagaa tttgatactg
     1201 agatgtgttt tttgcgtccg cagtgaccgc aagaatccgt cctgcttcgt ttcggaccag
     1261 gcgttcgatt ggctcatctt tgacaaaggc gattctcggc gccagcgagc aggcaggtag
     1321 caagaggagg agagctaaac ccccaatccg gagaacacct gctgagcgaa gtctgagctt
     1381 ccatgttgct atgttcaatg gatggttgtt cacgtcaatt ggccgtaagc gggccgcagg
     1441 aatttagcac aaattcctgc gtgccagcca gtcgtaatat attgaaaatc aagatgcaac
     1501 gcttttcatc cagccaatca gagctcagcg actgaagccg taaacgcgcg ggtggcacag
     1561 caagcacctg gtgagtgtga aaggccaaac catccgcggt agatattaac gcggtatggg
     1621 acgtttctaa cggctggact ttttgccggc gccggcctaa cgtttgtcgg atgcgccgat
     1681 ccctcttctt tattcttgct ttgatttgcg gcgggctggc agtctttgcc ctcagcctgc
     1741 tggtatttaa tagtgaatct ctcttactgc gtcatttgga acgaacccta ggtcgcaaga
     1801 ttttagcgcg ggaggtacag ataacgttta tacccgcact tggattacat ttaaaagagt
     1861 tctctatggc tgatgatcca atcttttcga gcggcgcttt tctgactgcg gatgctcttc
     1921 aggtgagatt caagtttctg ccgcttttcc tgtatcagct tcgcatcaaa gaaatcattc
     1981 ttcgcgaccc tgtcatcaat gtcattcgca atcatcgtgg tatttacaac ttttcttcta
     2041 ttgggctgga aaagccgagg aagacaatta tcgaacagcc cgtaaaatcc caagctcaag
     2101 cgaaagagta tgcaccggcg ctaataccta gctcatcgat tcaggtcatc aacggaagcc
     2161 tccgctatct cgatcagacc aatggcaccg atatgatagt tagccggctc gatctcaagg
     2221 tagtcgattt gcaagatagg aactctttcg cggtcgaact ggcgatggcg gtgtttgcga
     2281 tggaacagaa ttttcaactc aaagccgcgg taggccctct cgttccccaa agaagcttta
     2341 gagatctgcc gctcgacggt gagctacggt ttgaccgttt cgatatgggt aaactccgag
     2401 ctgtcttgcc tgtcatcaag aaaggattac cgaaggcttt ggaccttcgc ggcgtgtact
     2461 cgacccaagg tgtcagattc aaaggaacgt tgaaccggcc ttcgctaaag ggtcaggtcg
     2521 agggaacgga tgcatcattc cgtttcgact agtcatcgga agctctccca attcaagaca
     2581 aacttgtttt cgtagagcgt atccacatcg agaggaacag caatgccccg ctccttcagg
     2641 taatcggcaa tcgactttaa tggaaggcct accgccgcga gatagtctcc ttcaatggag
     2701 gcgatcaatt cacggccttt atcttggatg gaatagctgc cggctttatc gagggattca
     2761 ccgcaagcaa gataatcttg tattttggta tctgacatat cgcgcatctt cactgtgact
     2821 ctctcgaggg ccgtcaggcc aggcccaccg gatccatcga tgattcccac accggtgtaa
     2881 atacaatggg ttcgccctgc gagcttgtta agcattgcgc gagcgtccgt cagatcgcgc
     2941 ggtttaccga ttttctcgtg atcaagtgag atcatcgtgt cactgccgat cacaatggct
     3001 tccgggtgcc gcccggccac cgatttagct ttaccggtgg caaattccag aacctcttct
     3061 tcgatgctgc ggtgaaacga cagcatctct tcgaaatccg gctggattac atcaaacggc
     3121 aaaccgagga gcgcgagtat ttcgcggcgg cgcggtgagg ttgaagcgag aatcagcttc
     3181 atgaagtttt ggcgtgggct taaaggatga cgtggcgatc cggtgttaca acggctgccg
     3241 gaccttcatg ccggtcgttc gatctgcgct cggcttccgc cgattgcgcg atgaaattct
     3301 tcaagcactg ttcgacttcc ccggcagttt ttactcgaac catttgcgag cgcatttccg
     3361 cggcgccgcg gaatccgcgg caataccaag ttagatgctt tcgaattccg acaaaatttt
     3421 cgtccgatgc ctgctgcgcg tgatgttggc tatgctcgga gatgacgcga aagcgttcct
     3481 ccaggctaac cggcatgctg gcgatgcata gctgtcgctt ttccgcgagc gcgtgtttta
     3541 attgatgctt tgccctaaag atccatgggt ttccttgtgc agcgcgcccg agaagaacgc
     3601 cgtctacttc ggtctcgcgg aggcggcgct ggacctgcgc catatcctgc agatcgccgt
     3661 ttccaagaat aagagtttcg gaatcttcgc cgactttcac ggcgcgcgca atcgcgcccc
     3721 aatcggcgtc gcctttgtat ccctgctgca gggtgcgtcc gtggagggag atagcggccg
     3781 gattttcttc caacagagtt gcaatccaat cgtcgactac aatgcggtca taccccaagc
     3841 gagtcttgac cgataccggg atcggctggc gctcaagcgc aatatccgtt ccggtgcgta
     3901 accgattggc cattttgatc tttttaatga gttcaggttc tacttcaata tcactcagag
     3961 tctgtccggc gcaccagtct ttgatgccta ctttgaccgc tctgatgata gtaatagcca
     4021 ggcccggctc ccggatgagc gccgcgccgc agccggctgc cgcaaccttt cttgccggac
     4081 accccatatt gatatctaaa ccgtcgaaac cgagctcaca cacgatgtgc gcaaccttat
     4141 agaacagctc cggcgaacgc ccatagatct gcgcaactat cggccgttcg atttcacaat
     4201 aggtcaaatc tttaatcagc gtgtgcgctg cgtgaaaagc ggcttggata ttaacgaact
     4261 cggtaaatat gacatcgggc ccgccgtgtt tcgccgtgat atagcgaaac gacgcgtcgg
     4321 tgactccatc catgggcgag agaccaacaa taggcctgtt aatcaagcgc cagaaattgg
     4381 tttcaggaat cgattctaaa gatcgcgtag gtgacatcac tatcgggacc gcataactca
     4441 ggcggattat gaagcttaat ccatgtgagc tcctaaacca agcgatgaag cgccgggtga
     4501 taacatcatt cccaatgcat cgtcgatcgg caattcaacc gtggtcaccg gaaggttaaa
     4561 gtgaagatgg attcggtcga ccagtgccaa agctttcatc cgcgcgtttt tcagttacac
     4621 tatgatgtgc gtgcatggcc ttgaccttct gcaatcgtcg cgcgattgcg actcttcctg
     4681 ccgctcgtat cgatcctcct aagacaggag ctgaaaagca tgcgtaaatg ggcattcatc
     4741 ctcggcagta tcttgctggt cattgtgatc gccgctgtcg ttgccgtttt gaacatcaac
     4801 tcatacattc aacgcaatcg tgaatatctc ctccaacagg ccgaaaaagc tgttggacgt
     4861 caaatcgagg tgcaagatat tgaagtcaat ctgtggaacg gtatcggcat tcgttttgaa
     4921 aatttcagcc tggccgatga tccagcgttt tcttccaatc ctttcgtaca ggccaaaagt
     4981 cttcaggcca atgtcaggct tttgcctctg ctacgcaagg atgttcaggt caaaagactc
     5041 attctccacg agcctgggat caacatcatt cgaaataaac agggcgttta caacttcgcg
     5101 agtctcggta agccggataa gaagaaagac aaagaaacag aaggtgctcg cgataaatcg
     5161 gcgcgcgaac gtgccgccct tttgatttct ctagtggaga tatccggcgg cggtgcacgt
     5221 taccgcgacc tggcagatgg gacggacttg gcgattcagc aactcgattt acgggcacaa
     5281 gaccttaact ttgatcgccc tgtttcaatc aatctttcgg cggcagtttt ggcttcaaag
     5341 cagaatgtga aactcaggct actgatcgga ccgatctctc ccgaaagcga ctaccgcaat
     5401 atggcattgg atagccagct cgacatcgat cctttggatt ggagccagtt aagaaaagcg
     5461 gtgccgaagc tctccgccgc gttgcccaaa aacctggaac tcggcggcat ttacaaactg
     5521 accggcctta aactcaaagg aacactccag gatttcgcat tgaacggcag catcgacggc
     5581 acccaggggc ttatccaata tgctgattat tttcacaagc caccaggcat accgctagtt
     5641 tttgaaagcg ctgctcatta cagcaatgat aagattacca tccaaaacgg cggactacgg
     5701 cttcacactt tagcttcacg gttcaagggt gatgttgttc tgggcaacaa gccggtggtc
     5761 gctctattcg ttgatgccga cccggcgtcc ttagaaggtt gggagaaact aattcctgtg
     5821 gtggtcgatt atgacattaa aggcaaaatg ggtctgcgcg caacagtgcg cgggccagtc
     5881 ggcgcaggaa aaactccgca ggtggatggc aacctttctt tgcaaagtgc cagcattaag
     5941 attccggatc tgcccaaagc tatcgacaat atagatgccc aggtgaagtt ctcaggagca
     6001 cgcgctgaaa ttaaggaaac gaaccttaat ttaggcactt cgcatatccg tttggcggca
     6061 gtcatcgaaa aattctcgcc gcttacctta tcttataaat taagcagtgc cgagttggcg
     6121 ctggcggata tgcaaccttc gctgccggca gagcgtagca aagatgtcct tcgcgatctt
     6181 agcagtgatg gtcaggtgag cacggcagac ggccaaacga ttgtcacggg caagatcatt
     6241 tccggcgctg gaagactcta taatatggat tataagagcc ttgaggctaa tctctcctct
     6301 gcgaaaaaca gcgcgcttgt gcgtagtttt cgcgccaacc tgctaaacgg atccgtgcaa
     6361 ctcaagggag actatcgatt tgatgaacgt cccagattct ctacggcttc ggaaataaaa
     6421 ggcatcgatc tagtgcaact gtatcagtac ttcactcagg agcaaccgga catgcgaggc
     6481 agcttgaacg gcaatcttaa gttgtccgga caaggtgatc agtggcagga gattaaaccc
     6541 agtctccatg gagctggaca aaccgaagtg ctaaacggta ccttgttgaa tttcaatatt
     6601 gccgatgccg tgttgtcggg cgcaaccgga ataccgggcc tgacaaatct catcaatccg
     6661 gccgtcagaa aaaaatatcc tgaaacgttt gacgccaagg acacagtttt caaagaattg
     6721 cagacgcaga ttgatattgg cgaaagccgg gtaaacctaa aagatctccg tatcgcagcg
     6781 gcccagttca gggtcgatgg aaacggttgg gttgacttcg aacggcgcgt cgattttcgc
     6841 ggcgtggtcc gattctctcc tgcattgtct ggtgacatag ccggtgcggt ccgggaaatt
     6901 agattgcttt tcaataagaa caacgaactg gaaatccctt ttacattatc tggacaaatg
     6961 cccaatgtca gaccgcggcc ggacgcaagt ttcctgacaa agtcgctcca gcgggggcta
     7021 ttgcaaaaag gcgccgaaga acttcaagaa cagctcttcg gccccagaga acgccgtgcc
     7081 cctaatgatc gccagccgga ccaagagcgc cagcaaaagc caagttcacc ggaagacttg
     7141 attcgcagag gactagaagg gttgttcggc cgacagaagc gttaatgaga tgtgatgttg
     7201 tttcaccgat cgagcgtctc tttaccgact attccaagaa gcacggcaga aaaaagtagc
     7261 agaggcgcat acaccagaaa cggcagaccg ggattgtaag cgttcgcgag ataaccgccg
     7321 atcacgggcg caaggccact acccaactcg gcgacggttc gccttactgc ttgcaacctt
     7381 ccacgcgcgt gggaaggcac cacatcgtac gttgaagtcg ctatcgatcc caacgagagt
     7441 ccttgagcca aaccagacaa tccaacaaga atggataatt gggtaaagct agtcgtaaac
     7501 gggatcagaa cgaagaccaa tgcgggaatt ccggtgctcg gcaccgtaca ccactttctc
     7561 ccaacccgat ccataaccaa gcctgcggga ataatcatcg cgaaaacgat aagccccgat
     7621 atagaaaaca acatgccgat ctgagtgggc gatagttgga gataaaatcc ggcataaagc
     7681 ggtagtatgc tctgcaccag tatccgttgc gactggctgg cgacagtggc gagcacgaga
     7741 gccagatacg tggatcttag atctggccgt atttctttca acaacaatcc ggcgccgtgg
     7801 aatacttctc ggacccgact aaatcgccac gtgttattcg gcgggacctt ggatggcgcc
     7861 aatgccggcc cagtcgtccc gcggctcacg aatcccaatc caaccgcaag agcagtggcg
     7921 acagcatagc cgataaacgc cgctttatag ctgaacatgt ccgtcagcca accaccgagg
     7981 agtggacaaa cggccgcgcc caagttgtag gtgccatgca aactgctcat cacgcggccg
     8041 cgctgtttgc gatccgcgag atcgatacca gcaatctcgc gcgcgcttga ccagagacta
     8101 tcagcagcac ccataaccag caccagcacc aggatcgcgc tcagccaagg cgcaataact
     8161 gttgcaaacg atgcaacgct agccactaac ggcccggcaa tgagccccac gcgcgttccc
     8221 atgcgatcca gaactatccc ggcgatcacc gttccaacga atttgccaat cgcaaaggct
     8281 gtcacaatct gcgccgcgcc tcccgccgaa acatcgaact cccgagcgat tactggaatc
     8341 gtcgggatga ccattgccca tgcgccggat aaaaaagtag cgctgtaaag tgccgcgagt
     8401 ggccgggcag tggaggtatt cataggccag gaagtgagca cgcaaagaga aaacagcttt
     8461 ataccgttga agaccaacac gccggaggct gaacatccaa gaatgggtgc ccccctggaa
     8521 gtcgtggtat cgtgggatct gttactgcag gacctgtgag actcgctaac tcgttgaagg
     8581 cagggagcct ccatccacac gttaacctac cgctttcgag cgcgtatcga acgggcattt
     8641 ggccatgcca cggcttcttt gctgggactg cgttcccata caaggccagt ctttttgtcc
     8701 agcaccgcgt cggcatcaaa ctcgttgagg acttcgaacc gccgcctcgt gttatcgatt
     8761 ctacggtccc agctagagct cgttagcctg acctcctaaa aagagacatt tgcccctata
     8821 ataacgcatt gtaggctcag cagggggcta aatccgagcg ccgagaccat gagtccctcg
     8881 aggcacgcag gtttgccgtt gtcgagttct gacagtgtcc ctgcttcgca ctccagcctc
     8941 agtgcgtcga ttgtccgact cctcaattag attgtccggg gtctctgact gtcgcggagt
     9001 tc
//
LOCUS       DASUVS010000151        23005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1149885, whole genome shotgun sequence.
ACCESSION   DASUVS010000151 DASUVS010000000
VERSION     DASUVS010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 23005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23005
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149885"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..516)
                     /locus_tag="VF182_08425"
                     /pseudo
     CDS             complement(304..516)
                     /locus_tag="VF182_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084520.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
     gene            743..1231
                     /locus_tag="VF182_08430"
     CDS             743..1231
                     /locus_tag="VF182_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437144.1"
                     /translation="MDLRVAKRTAFLLFIGFLGVITLGATTDKTVYSHISNRELKRKS
                     LQLATDVRRLVDSYKKRDREIMSDYDRKDRPEMSQAGTHELRQQWLKDSDRAHDSTMR
                     YYKDHYWADAILLIDELNRRLSKRAKQPELLRLYQHPTNVLGLAAVADHLELHSKLLP
                     NS"
     gene            1328..1537
                     /gene="rpmE"
                     /locus_tag="VF182_08435"
     CDS             1328..1537
                     /gene="rpmE"
                     /locus_tag="VF182_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX6437145.1"
                     /translation="MKEGIHPEYKQAQVICACGFIVETRSTVGNIHVDICSNCHPFFT
                     GKQKFIDAAGRVEKFRKRYAPKSEA"
     gene            1668..2732
                     /gene="prfA"
                     /locus_tag="VF182_08440"
     CDS             1668..2732
                     /gene="prfA"
                     /locus_tag="VF182_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX6437146.1"
                     /translation="MLDKLAEVEKRYEELERLMSDPGLANLQREYSKLAKERSELEEI
                     VASYREWRKVEQEIQENRQLLEENDEAIRELAKEEVSALRQREEELENRLKYLILPKD
                     PNDSKNVIIEIRAGTGGDEAALFAGELYRMYSRYAESRGWRVEILTSSPTGLGGFKEI
                     IVMVEGKGAYSRLKFEGGVHRVQRVPVTEGSGRIHTSAVTVAVLAEADEVELDIDPKD
                     IRIDVFRSSGPGGQSVNTTDSAVRITHIPSGIVVSCQDEKSQHKNRAKGMKIMMARLL
                     EKKQEEQRSEIAATRKLMVGSGDRSERIRTYNFPQGRLTDHRINLTIYQLERIMEGQI
                     DEIVDGLITHYQAEALKATG"
     gene            2741..3655
                     /gene="prmC"
                     /locus_tag="VF182_08445"
     CDS             2741..3655
                     /gene="prmC"
                     /locus_tag="VF182_08445"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX6437147.1"
                     /translation="METDSQSLGSLLGPRTIRCDLVDGTRRLQAAGIESARLDAEVLM
                     CHALRIKKSRLYVGLDEQPESSARQRFSNFLIRRLRREPVAYITGRQEFWSLDFLVTP
                     DVLIPRPETERLVEISLQCAGIFDNDLPLRILDIGTGSGAIAVSLAKELPRAQVLAID
                     SSRAALEIARDNAARHQVADRVHFRASNLFESIRGREFHLIVSNPPYVESGEFAALAP
                     EVSEWEPRTALDGGVDGLHYYRDIVAQAFRHLPPGGALIMEIGAAMARAVADLLSGAG
                     NYTAPLVHKDYGHRDRVIVAHAASSKLA"
     gene            3664..4932
                     /gene="murA"
                     /locus_tag="VF182_08450"
     CDS             3664..4932
                     /gene="murA"
                     /locus_tag="VF182_08450"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX6437148.1"
                     /translation="MDKIIVQGGKRLTGEVTVSGSKNAALPILIASLLTAEPCRYQRV
                     PSLADIHTTLKLLGGLGVKIDKEGWARRSGELTLQAQTVTKLEASYDLVKTMRASFLV
                     LGPLTARFGQARVSTPGGCAIGARPVNLHLKGLESMGATIEHSHGYIEATAVRLRGAK
                     IYLDLPSVGATENLMMAATLAEGTTVIENAAKEPEIEDLAHALNKMGASVRGAGTDIV
                     QIDGVDSLHGFSHEIIPDRIEAGSFVIASAITGGDVWIKGARAEHLDAFLIKLQEAGI
                     ALRADRGGIRVQRNGRIKSVDVTTLPYPGFATDLQAQMMVLMSVADGVSIITETIFEN
                     RFMHVQELDRMGAQIQLEGNRAVVRGTRELSGAPVMATDLRASISLILAGLVASGSTE
                     VSRVYHLDRGYEHIEEKLSNLGAQIERVKA"
     gene            4979..6274
                     /gene="hisD"
                     /locus_tag="VF182_08455"
     CDS             4979..6274
                     /gene="hisD"
                     /locus_tag="VF182_08455"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX6437149.1"
                     /translation="MKVRIYKTSEPAYRSLIRRILQRRRGRQGDVEQRVNEIIRSVQR
                     EGDRAVLRYTQEFDRRRMTRATMEVRAGEIERAMAKVPARDLGTLRVAARRIAAFHRR
                     QLQKTWHYRDSLGLVLGQIITPLERVGVYVPGGKATYPSTVLMNVMPAKVAGVKEIVM
                     TSPMGSDAPIILAAAKIAGVDRIFRIGGAQAIAALAFGTATIPRVDKIVGPGNIFVAT
                     AKRLVFGEVSIDSIAGPSEILLLADDSADPAHVAADMLSQAEHDELAAALCITSSPPL
                     AARIQRALEEQLRLTQRRKITMKSLQNYGAIIVTRDLTEAVRIANEIAPEHLELLVRK
                     PEKWAPKIRNAGAIFIGPYSAPPLGDYLAGPNHVLPTGGSARFFSPLGTYDFLKRTTV
                     IHADKSALRSLAPKIIQLARLEGLDNHARAIEARFSKEG"
     gene            6277..6864
                     /gene="hisB"
                     /locus_tag="VF182_08460"
     CDS             6277..6864
                     /gene="hisB"
                     /locus_tag="VF182_08460"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX6437150.1"
                     /translation="MGRTGTVRRKTKETEIQVAVDLEGSGKAEIETGMPFFNHMLDSF
                     SRHGFFDIKVQAKGDLEVDYHHTVEDVGLALGEAFKNALGDKHGIRRFGEASCPLDET
                     LAKVVIDLSGRPYLSYNVKIRPGRVGSFDTDLPHEFYAAFTNQLGMNLHIDVIRGENP
                     HHIIEACFKAFARAMDMATGLDQRISGVLSTKGSL"
     gene            6926..7543
                     /gene="hisH"
                     /locus_tag="VF182_08465"
     CDS             6926..7543
                     /gene="hisH"
                     /locus_tag="VF182_08465"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX6437151.1"
                     /translation="MIAIIDYGMGNLRSVQKALERVGYEAMLTRDPGQIESARGVVLP
                     GVGAFSACMENLSRFGLIQPICDIIGRKKPFLGICLGFQLLFSESEEFGRQKGLDLFA
                     GKVVGFHSTDGLKVPHMGWNRLVKKKDSPFLEGVPNGEFVYFVHSFYVVPEDASVIAT
                     DTEYGSPFVSSIATDSLFACQFHPEKSQEIGLRILSNFGRFVASN"
     gene            7547..8269
                     /gene="hisA"
                     /locus_tag="VF182_08470"
     CDS             7547..8269
                     /gene="hisA"
                     /locus_tag="VF182_08470"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX6437152.1"
                     /translation="MIIIPAIDLKHGRCVRLFQGKMDQETVYFEDPLEAARHWIREGA
                     TIIHIVDLDGAVEGRPVHTKQVEAICRRASLAVEFGGGLRSMEAVEAAFELGVSRVVI
                     GTAAYDDPGLLRLLCEKFPGRIVVGLDARQGKVAVKGWKETTPMNAVELAQRCEQDGA
                     SRIIYTDISKDGTGEGVNADETLKIARAVTIPVIASGGVASLQDLRKLAPLESEGVEG
                     VVIGRALYARAFTLLEAKSIFA"
     gene            8441..8788
                     /locus_tag="VF182_08475"
     CDS             8441..8788
                     /locus_tag="VF182_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437153.1"
                     /translation="MKDKKYSVLIAVILLLSFAAMPGMSIAQEANERSTGPGFFSVLG
                     SIVFTTLHVPFKAVTCAGTQVTSAVAYAGTFGVEGHYDGGTNGRDIGETARRSCTGSW
                     IVRPSQVAKDYGE"
     gene            9169..9930
                     /locus_tag="VF182_08480"
     CDS             9169..9930
                     /locus_tag="VF182_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEX6437154.1"
                     /translation="METIPPVSFEGFYFSVASSAAWAPQIETRDAWLAWAFDHEVAAG
                     RSTPSLEAMPSILRRRAGFLGKMALEVAYRCLGARTDIPTIFSSRHGEAARSADLLLD
                     LADGTALSPTSFSLSVHNAAGGLFSIARGDRASNMALAAGVSTVEHGVIEACGLISEG
                     APAVLLVVYDCPLPALYADFEDCDEQPFAWAWLIESAAGEVLSLRCEPGSGVGPSRAK
                     GMPAGLEILRFFLRKDSALDRVCNKRRWLWSRHVQ"
     gene            9920..10690
                     /locus_tag="VF182_08485"
     CDS             9920..10690
                     /locus_tag="VF182_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6437155.1"
                     /translation="MFSRIGRWWRVFGTGVSFVAVGVGGVFFFPVLNIVIHQPQLRSM
                     LARRVISFSFRCILGLMWIMGVFRYEITGMERLKRKGLLILANHPTLIDIVFLMAFVK
                     RADCIVKSGLWRNPFTHSTIRAAAYVRNDDHGVQLIEDCVGAIRRGGNLIVFPEGTRT
                     PSNGSIVLKRGAANIAVRGLCNITPVLIRCAPPMLAKGERWWRLPFRPSHFTITVKED
                     IEVKPFIARAGSEPLAIRHLTDHLQEYFAKEQPPDAIG"
     gene            10677..10937
                     /locus_tag="VF182_08490"
     CDS             10677..10937
                     /locus_tag="VF182_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX6437156.1"
                     /translation="MQSDDDEIKQMIIDVLQLEDVTPADIDTDAPLFVEGLGLDSIDA
                     LELGVAIHKRYGVTLSADSDDTRRHFASVRALGVMIDKHRRK"
     gene            10948..11211
                     /locus_tag="VF182_08495"
     CDS             10948..11211
                     /locus_tag="VF182_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX6437157.1"
                     /translation="MTPASPVSEEEIYPWLIDLLHDMFDLDKAKITCEANLYTDLDID
                     SIDAVDLAVKLKQLTGKRLQPEVFKNVRTVQDVVNALAGLLLK"
     gene            11215..11766
                     /locus_tag="VF182_08500"
     CDS             11215..11766
                     /locus_tag="VF182_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437158.1"
                     /translation="MRRFLALWAPSIMILYPFAVWLGHGKVEPRFFAGLLLIAGFMRL
                     PLMKAGAAGTGWLAGTLLLSLIAFWGNHVLTLKLYPVLVNGVMLAVFTYSLFIPPSII
                     EQFARRREPNFGPRSVRYTRRVTQVWCVFFALNGAVALITALWTSAATWSLYNGVIAY
                     LLMGVLFAGEYGARRNFKRRLNA"
     gene            11759..13486
                     /locus_tag="VF182_08505"
     CDS             11759..13486
                     /locus_tag="VF182_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6437159.1"
                     /translation="MPETLDMLSVAVMPRPLSQTIAWRDQQPVSYGEFFHRTRAWQQL
                     LSRISGQTFALSLSDAGEFAAALFGAWQAGKIIYLPGDRLPGTCHAMRSLVDGYLGEF
                     APEWNPRTPTPEDGLAPGAELKPLDPNFVGLFIYTSGSSGAAQAISKKLSQMSAEVAT
                     LEAQFGDIIDDSDIIATVSHQHIYGLLFNVLWPLSAGRPFAARSFGSLEGLAMALARR
                     DAVLVSTPAHLRRLPETPDWELASKRVRAVFSSGGPLPADAARESSQLLGQVPIEVYG
                     SSETGGIAWRQQKTRHDAEWTPLPNIKWRIDAEAGVLEIRSAHLPDAEWFRTADRARP
                     AGGNRFLLEGRVDRVAKVEGKRISLDAIEERLKASPLARDARALVIEGRRQRIAAVVV
                     PSATGWHKLAHRGKLAVNRMLRGLLRETIEPAGLPRLWRYVDALPMNTQGKTTHAALI
                     DLFDNRDNRPRQAIECRVHKVDQHAIFELIAPSNLLYFDGHFRDVPILAGVVQIEWVV
                     AFGRRCFDLPPLFRAIHALKFQRVIPPELPITLELAHDPLKSSLAFKISSHLGAHASG
                     RIIFRAADV"
     gene            13479..14222
                     /locus_tag="VF182_08510"
     CDS             13479..14222
                     /locus_tag="VF182_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6437160.1"
                     /translation="MFKPCVVIPVFDHERAVGAVVTEVLAQNLACVLVDDGSSSRCSS
                     RLHEMAAARPQMITLLRHPMNRGKGCAVLTGLRYAAEAGYTHAVQVDADGQHRIADIP
                     RFLEQALAHPNALIVGHPDYDSTVPALRLYARQLTHIWVSINTLSRQIKDSMCGFRVY
                     PLAPVIALDQRINLGARMDFDTEVLVRLYWDGLHILNVSTPVTYPADGISHFRGCLDN
                     ALLSRMHATLFFGMLLRLPVLIARKCRRQ"
     gene            14219..15175
                     /locus_tag="VF182_08515"
     CDS             14219..15175
                     /locus_tag="VF182_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX6437161.1"
                     /translation="MIQVIAARRIRHWAQINECSFVAGIYLLFWITRVFGRWPLRAAL
                     YPVVLWYMMTRPAARAASRDYLRRLADSGGQVILKPDVFSVFRHFACFAESILDKMLL
                     WSGLYKTDQVKFVGKELIMAQTAAQSGGLLICCHLGNSELGRFLSKQLPGFKLTVLIH
                     TKHAQRFNRLLAQLDPESQVDLMQVTELSPATAIVLAEKLAQGEFVAIAGDRIPVSAN
                     PRVALAEFLGTVAPFPAGPYILASLFHCPVYLMFCRSYGGTAEIHFELFHQVIDLPRI
                     GRDQALARVAGEYARRLEHFCRRAPLQWFNFYDFWSLSSVEN"
     gene            15177..16733
                     /locus_tag="VF182_08520"
     CDS             15177..16733
                     /locus_tag="VF182_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HEX6437162.1"
                     /translation="MESPAFKKTTRTVRFDREWVKIQDIVDVADGLAWPVLSETPQFR
                     SAIAQGAEFVDRLLREDGRIYGVTTGFGDSCMVTVGTELAAELPRHLYTYHGCGLGEY
                     LTPAQTRAVIATRLTSLCKGFSGVSVELLEQMVRLLHCGVLPRIPSEGSVGASGDLTP
                     LSYLAAVLCGEGEVWREGAAAPAAVALARARIPPLSLRPKEALAIMNGTAVMTALACL
                     AYSRAEYLSRLVTRVTALASFALDGNAYHFDETLFSVKPHPGQQRVAAWLRADLLCDQ
                     PPRNATRLQDRYSIRCAPHVIGVLADALPWLRESIENELNSANDNPIIDAAGERVLYG
                     GHFYGGHIAFAMDSMKNAVANLADLLDRQMALLVDSRFSNGLPANLSGIEGPRQAINH
                     GLKALQISASAWTAEALKLTMPASVFSRSTECHNQDKVSMGTIAARDCLRVLELTEQV
                     VAALLITVRQGVWLRCRSNRSLRLQAALQTMMHQLENDVVALEEDRRLEPDLRLLLER
                     VRSRAWELYE"
     gene            16726..17154
                     /locus_tag="VF182_08525"
     CDS             16726..17154
                     /locus_tag="VF182_08525"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX6437163.1"
                     /translation="MSKPSSRWIAETETQIEFFDLDPMEIVWHGRYVKYLEMVRGVLL
                     DTIDYNYPQMKASGYVWPVIDMHLRYIAPAIFGQRIKLRAEIVEWENRLKIGYLITDA
                     QSGRRLTRASTTQVAVEIATREMCFVSPRVLLERMGVGPC"
     gene            17148..17753
                     /locus_tag="VF182_08530"
     CDS             17148..17753
                     /locus_tag="VF182_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX6437164.1"
                     /translation="MLRRQLVVFWLVLSAPPLLGATPLSTIESMLLKPTVLCGRFEQK
                     KQLVGFKNAVNSSGRFCVVAGKGVLWRTLKPFPTALRLNRDEIVQLHGDRVTLRLEAK
                     KEPTVRMINTVLFALLAVDIGQLEKHFEIDGSVQNNVWSIRLKALEPALAKAIGSIAF
                     EGGVYVRSIRIDEASGDRTSIDLMAIETGESAMDADEAKLF"
     gene            17750..20134
                     /locus_tag="VF182_08535"
     CDS             17750..20134
                     /locus_tag="VF182_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX6437165.1"
                     /translation="MIRLKLLAWFWSLVVCLLVVHNSYLWLHQRLAPETDILALLPLQ
                     QRDPVLQQAFTRMVDTGQQRLIVLIGADDWSQARDAADAYHSELLAYKDLLQLEHRAT
                     FDSQQEWLGLFEQHRLGLLTREQESALHQEPAQFWIDRALSKLYRPFGGSMLTAWHDD
                     PFNLFGEWAQARAQETAVRPREGRLSISEGQREYVVMPFTLRVAAFSIAAQQSIIPLL
                     QRAKNNARKAVPGVEVLSAGLILYADAAAEQSKLEVGTIGLGSMLGIIVMTWIAFHSL
                     KPIAWVIVSVVIGCLGALSICWAVFDRIHLLTLVFGASLIGGAQDYGTYFLCNRFAAD
                     ARQLNSWQLLRRLMPALVLALVTTVIGYLGLALTPFPGLQQVAVFSAIGLVFAWLTVI
                     CWFPTLVWRGTLKGGRFAEYYATTMQQWPVLRWNQRTVAVGLVSIAFASCGFSALHTE
                     DDIRSLQNPPKHLVDEQIKLGRLLDAPAPAQFYLIRGPTAEAVLQREEALTKRLEPMI
                     EKRLITGYQAVSNWVPSLAAQLERRQLIQEKLLDDSGPLDMLAQKLGEGGKWGSGSRQ
                     GLVASALPLSPDEFFKSPISEPIRPIWLGNVNGTYASIVVLRGVKKDTLATLQHAAGR
                     LEGVQWVDKVSEVSSVLAAYRHYMSWLIVFSYFAVYGLLYPRYGRATWRVVAPTAIAS
                     LATLALLGSAGAGVQLFHVLALMLLLGIGIDYGIFLQEHFGRRDAIAWLSVALSAINT
                     LLSFGLLSLSKTPALQAFGLTMLIGTVILCLIVPCFVVGITDTRVLTERFGASA"
     gene            20131..21381
                     /locus_tag="VF182_08540"
     CDS             20131..21381
                     /locus_tag="VF182_08540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6437166.1"
                     /translation="MRVTGLPQKVGKSVEILIIGAGPAGSVAASLLRQQGRQVLMLER
                     EQFPRFSIGESLLPQSMEYLERCGMLRAVVEAGFQLKNGAAFAQGEQYTEFEFGEKYS
                     TGWNTTYQVPRARFDHLLALEAEAQGAEVRYRHEVIGIDLGGSRPRLTIKDSDGEHYL
                     VDASFVLDASGFGRILPRLLQLETPSNFPVRGAVFTHIEDRITHPGFDRNKIRVTVHP
                     ERPGVWFWLIPFAAGRCSLGVVTEPALLDRYQGPDTQRLQALVGEDQTLSNLLENACW
                     DTPARRVVGYAANVKSLSGRDYALLGNAAEFLDPIFSSGVTIAFKSASLAAAVLQRRF
                     AGETIDWLSEYAVPLKIGVDTFRAFVDSWYRGGFQRIIFHRNQPPEIRRMISAILAGY
                     AWDESNPYVREPRRRLAALEELCS"
     gene            21372..21935
                     /locus_tag="VF182_08545"
     CDS             21372..21935
                     /locus_tag="VF182_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HEX6437167.1"
                     /translation="MFVALPGRQVALVGSLTLLLTGCVAPSPRAAGVGLKLAPAALAE
                     SISLQQRLDIEGQGRRAQLDVVLEVDAERIDLVGLAFGQRVLSMSYDGKTLQSWRHPM
                     WPAQVRAEEVLENIQLTLWPVDAIRRALPMGWSIKESGLRRTLLQDGLAVMVIDYSAL
                     PFWSGKVEVANLQFNYRITIQSVSNEP"
     gene            21936..>23005
                     /locus_tag="VF182_08550"
     CDS             21936..>23005
                     /locus_tag="VF182_08550"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEX6437168.1"
                     /translation="MIYLNQLGIVCPLGSSREEVKHRLLDRVASGVSLTENYSPGRLL
                     PLGCIDAELRLPSVDHLDLIARSRNNQVALAALAQIRTAVDSAIEQYGKGRFGIVIGT
                     STSGIAESEAAFRHYVWNRSLPEQFHYGQQELNSLATLLADELDVWGPAYVHSSACAS
                     SAKALASAARLIRTGACDAVLTGGVDTLCGFTVAGFAALESVSATQCNPLSRNRRGIN
                     IGEGGALFLMSREPASVALRGWGESSDGYHMSAPDPSGAGARLAIERALSRAEISAES
                     IDYINLHGTGTIQNDAMEARVIHALFGAAVAVSSTKPLTGHALGAAGAIEAGLCWLAM
                     QDDNPAGRFPPHLWDGIPDPTLP"
BASE COUNT         4807 a         6065 c         6662 g         5471 t
ORIGIN      
        1 agcgacacag ccatgtcaca ccgggtccca acacgctaat cttgctaaat ttaaagttct
       61 gcgacttcaa ctcttgtaag ctctttagga caacagcatc gtcgcgatca agagaaattc
      121 gactagcagc agaaacagat aaacccatcc ataacgtctg aggtgattga cgattttata
      181 gttgctcttc aagaccggtg cttgaggttc cggggctttg ggggttggtg gtcgagaagt
      241 tctatttgcg gccggacgtg ttctcggtcg gggcttggct ggcggttcag tttcgacctt
      301 ccggatccac tcgtcatgtt tccttcgtag atcaggatcg gagagaaccc tgtaggccgc
      361 attgatgatc gccatggttc tggatgccct cgggtcaccc gggttccgat cggggtggta
      421 cttctgggac aaggatttgt aggcggcgcg gatcacttca ggcggcgcat ctcgtgctac
      481 ttttaagttg tcgtagtgcg tgcgcacctt aggcacgagt cctcccccag aatgagcaac
      541 tccttcgaac ctgtatcatt agcatttcat ccggacaatt gcccgactcc tcgcaagctt
      601 agtttagcag attgcacagc cgcccggttg ccgtcttgtt tgccctcgca cccgctgcat
      661 attatgactg gaaaacggcg ggaaaccagt atgaacgaag ggtcactgat ctattcccgt
      721 gcagttagga ctcaaactat ccatggattt gcgtgttgcg aaacgtacgg cttttctctt
      781 gtttatcgga ttcctggggg tcatcacgct tggagcaacg acggacaaaa ccgtctattc
      841 ccacatatcg aacagagaac tcaagcgaaa atctcttcag ctcgccacag acgtccgccg
      901 cttggtcgat tcttacaaga aacgggaccg cgagatcatg tccgactacg atagaaaaga
      961 ccggccggag atgagtcaag ccggaacgca cgaattgcgg cagcagtggc tgaaagactc
     1021 ggatcgggct cacgattcaa cgatgcgtta ctacaaagat cattattggg ccgatgccat
     1081 attgctgatt gacgagttaa accggagact gtcgaagcga gcaaagcaac ccgagctcct
     1141 cagactgtac caacatccca ctaacgttct tggtctggcc gccgtagctg accatctgga
     1201 gcttcattct aagttattgc cgaactcctg acctcctgca cgggattgct ccaacgtccg
     1261 tcgaattggg gcgcgatttg aagagcttcc atcagtgtgt tataaccagc gtgatcgtga
     1321 ggtagttatg aaagaaggca ttcatccaga gtacaagcag gcgcaggtga tctgtgcttg
     1381 tggctttata gtggagaccc gttccaccgt cggcaacatc cacgtcgata tctgttccaa
     1441 ctgccaccct ttctttactg gaaagcaaaa attcatcgac gcagcaggac gcgtagagaa
     1501 gtttagaaag cgatacgcac ccaaaagcga agcctgagtt cagcggagga ttggggctga
     1561 actgagagat ttaacagttt ccagcgcgga aagggcaaag agtcgatcat actctctttg
     1621 cccttttttt atcttctcgg accaggcaac tccccaggtt aaatcctatg cttgataaac
     1681 ttgcagaagt tgaaaaacgt tacgaagagt tggaacgttt gatgtcggat ccggggctgg
     1741 ccaacctgca gcgggaatat tcaaagctag ccaaggagag atctgaattg gaggagatcg
     1801 tcgcctccta tcgtgaatgg cgcaaagtcg agcaggagat ccaggagaat cgtcaactcc
     1861 ttgaagaaaa tgacgaggcc ataagagagc tcgcaaaaga agaggtcagt gctcttcgac
     1921 aacgtgagga agagttggaa aaccgtctca agtatctgat cctgcctaaa gatccgaacg
     1981 acagcaaaaa cgttattatc gaaattcgcg ccggcacggg tggcgacgaa gccgcactct
     2041 ttgccggcga gctttaccgg atgtattctc gctacgctga atctcgcggc tggcgcgttg
     2101 aaattttgac ctctagtccg accggtctcg gcggctttaa agaaattatt gtgatggttg
     2161 agggaaaggg cgcctatagc cggctgaaat tcgagggcgg agttcatcgc gtgcaacgag
     2221 ttcccgtcac tgagggttcc gggcgtatcc atacctcggc agtcacggtt gcggtgcttg
     2281 cggaagcgga tgaagtggaa ctcgatatcg atccgaagga tatccgcatc gatgtttttc
     2341 gctcttcagg gcccggtggc cagagtgtca acacgaccga ttcggcggtg cggatcacgc
     2401 acatcccgag cggcatcgtg gtgtcgtgcc aggatgagaa gtctcaacat aagaacagag
     2461 ccaaagggat gaaaatcatg atggctcggc tattggaaaa aaaacaggaa gaacagcgct
     2521 cggagattgc cgcaacgcga aagctgatgg ttggcagcgg cgaccggagt gaacggattc
     2581 gcacctataa cttccctcaa ggaaggctga ccgatcatcg catcaatctg accatttacc
     2641 agctcgaacg tatcatggaa ggccaaatcg atgaaattgt cgatggactg atcacgcatt
     2701 atcaagcgga ggcgctgaaa gccaccgggt gatgacttcc atggagacgg acagccagtc
     2761 attaggttca ttgctgggac cacgaacaat ccgttgcgac cttgtcgacg gtacccggcg
     2821 acttcaagct gcaggaattg agtcggcgcg tcttgatgcc gaggtgctga tgtgtcacgc
     2881 cttacgcatc aaaaaatccc gcctttacgt cggtctcgat gagcaaccgg agtctagcgc
     2941 gcgacaacgg tttagtaatt ttttgatccg ccgtttgcgc cgcgagccgg tagcctacat
     3001 caccggtcgt caggagttct ggtctcttga tttcctggtt acgcccgatg tcctcatacc
     3061 gaggccggaa accgaacggc tcgttgagat ctcacttcaa tgcgccggca ttttcgacaa
     3121 cgacttgccg ttgcgaattc tcgatatcgg caccggcagc ggcgccattg cagtcagtct
     3181 cgcaaaggag ctgccgcggg cgcaagtact ggctattgat agctcacggg cggcgctcga
     3241 gattgcgcgc gacaatgccg cgcgtcatca agtagcggat cgagtccact tccgtgccag
     3301 caatttgttc gagtcaatca gagggcgtga gttccatcta atcgtctcca atccgccgta
     3361 cgttgagagc ggtgagttcg ccgctttggc gccggaggtt agcgaatggg agccacgaac
     3421 cgcattggac ggcggtgtgg atggactcca ttattatcgc gacatcgtag cccaagcatt
     3481 tcggcatttg ccgcccggcg gcgcattaat catggaaatt ggcgctgcta tggcgcgagc
     3541 ggtcgcggac ttattgagcg gcgccgggaa ctatacggcg ccgttggttc acaaagatta
     3601 tggccataga gatcgcgtga ttgtcgccca cgctgcctcg tcaaaattag cctagagctt
     3661 ctaatggaca aaatcatcgt gcaagggggc aagagactca ctggtgaagt caccgtcagc
     3721 ggttcgaaaa atgccgcgct gccaattctg atagcatcgt tactgacggc ggaaccctgc
     3781 cggtaccagc gagtcccgag tctggccgat attcatacga cattgaagct tctcggcggg
     3841 ttgggtgtaa agatcgacaa agagggctgg gcgcgaagaa gcggcgagct cacattgcag
     3901 gcccaaacgg taactaaact tgaagcctct tacgacttgg tcaaaaccat gcgagcttcg
     3961 ttcttggtgc taggaccttt gacggctcgc ttcggccagg ccagagtctc gactcctggc
     4021 ggctgcgcca tcggcgcacg tccggtcaat ttgcatctca aaggccttga atcaatgggc
     4081 gcgaccatcg agcactccca tggttatatc gaggcaaccg cggttcggct gcgcggcgca
     4141 aaaatctatc tcgacttgcc ttccgttgga gccaccgaaa atctcatgat ggcggcgacg
     4201 cttgcggagg gtacaacggt tatcgagaat gccgctaagg aacccgagat tgaggatctg
     4261 gcccatgcgc tcaacaaaat gggtgcgagc gttcgtggtg cgggcaccga cattgttcag
     4321 atcgacgggg tagattcgct gcatggattc tcgcacgaga ttattcccga tcgcatcgaa
     4381 gcgggcagct tcgtgatcgc cagcgccatc accggcgggg atgtctggat caaaggcgcg
     4441 cgcgccgaac acttggatgc tttcctgatc aagttgcagg aggcgggcat cgcgcttcgt
     4501 gctgaccgcg gcggaatcag ggtgcagcga aatggcagaa tcaaaagcgt cgatgtaacg
     4561 actctaccct atcctgggtt cgcaaccgat ctgcaagccc agatgatggt tcttatgtcg
     4621 gtcgccgacg gcgttagcat catcacggaa acgatctttg aaaaccggtt tatgcacgtg
     4681 caggagttgg accgtatggg agcccagatc cagctcgaag gcaatcgcgc cgttgttcgc
     4741 ggcaccaggg agctttcggg agcgccggtg atggcgacgg atttgcgcgc tagtatttcg
     4801 ctgattcttg ctggactggt ggcaagcggg tccactgaag tctccagggt gtaccacttg
     4861 gaccgcggct atgaacatat cgaagaaaaa ttatcgaatt tgggcgcgca gatcgagcgg
     4921 gttaaagcgt aagtaacccg gtcgagtttc tatttctccc ggaaagagaa tcgatttcgt
     4981 gaaggttagg atctataaaa catctgagcc ggcctatcgc tcactgatca gacgaattct
     5041 tcaacggcgg cgcggtcgcc aaggcgatgt cgaacagcgt gttaacgaaa tcatcaggtc
     5101 agtacagcgg gaaggcgacc gtgcggtgct gcgttacact caggagtttg accgaaggcg
     5161 catgactcgc gccaccatgg aagtacgcgc cggcgaaatc gagcgagcca tggccaaagt
     5221 gcctgccaga gacctcggca cactacgtgt cgcggccaga cggatcgccg catttcaccg
     5281 gcgccaattg cagaaaacct ggcattatcg cgattccttg ggattggttc tcggtcaaat
     5341 aatcactccg cttgagcgag tcggagtata tgttccgggc ggcaaagcta cttatccctc
     5401 aaccgtgctg atgaacgtga tgccggcgaa agtggcaggg gtcaaggaaa tagtcatgac
     5461 ctcaccaatg ggcagcgatg cgccaatcat cctggcggcc gctaaaattg ccggtgtcga
     5521 tcgaattttc cgcatcggcg gcgctcaggc aattgcggcg ttggcgtttg gcactgcgac
     5581 aattcccaga gtagacaaaa tcgttgggcc gggaaatatc tttgtcgcaa ctgccaagcg
     5641 gttggtgttc ggcgaagtca gcatcgattc gatcgccggg ccgagcgaaa tcttgctgct
     5701 cgctgatgat tctgccgatc cggcgcatgt cgcagcggat atgctctcgc aggccgaaca
     5761 tgacgaattg gcggcggcgc tgtgcatcac ctcatcgccg ccattggcgg caagaattca
     5821 gcgggcactc gaagagcagc tacgtctgac tcagcgacgg aaaattacga tgaagtcatt
     5881 acagaattat ggcgccatta tcgttacccg tgacttgaca gaagccgtcc ggatcgccaa
     5941 cgagattgcg ccagagcatc tggagctact ggtccgaaag ccggaaaaat gggcaccgaa
     6001 gattcgcaac gcaggcgcta ttttcatcgg cccctattcg gcgccgcctt taggagacta
     6061 tcttgccggc ccgaatcatg ttcttccaac cggcggcagc gcgcggtttt tttctccttt
     6121 gggcacctat gattttctta aacgaaccac ggttattcac gccgacaaga gcgccttgcg
     6181 gagtttggct cctaaaatca ttcagctggc gcgactcgaa ggcctcgata atcatgcccg
     6241 cgctatcgag gcgcgatttt cgaaggaggg gtgacaatgg gccgaaccgg cacggttaga
     6301 cgcaaaacca aggaaaccga gatccaggtg gcggttgatc tcgaaggtag cggcaaagcc
     6361 gagatcgaaa ccggtatgcc gttctttaac catatgttgg atagcttttc ccgccacgga
     6421 tttttcgaca taaaggttca ggccaagggc gatttagaag ttgattatca ccacactgtc
     6481 gaggatgttg gtttggcatt gggagaagcg ttcaagaacg cattggggga taaacatggg
     6541 atccgacggt ttggcgaggc aagctgcccg ctggatgaaa ctttggcgaa agtggttatc
     6601 gacttgagcg gccggccgta tctctcgtat aacgtgaaaa ttcgccccgg tcgcgtcggc
     6661 agtttcgata ccgacctgcc gcatgagttt tatgccgcgt ttaccaatca gctggggatg
     6721 aatctgcaca tcgatgtgat tcgcggcgaa aatccgcatc acattatcga agcctgtttc
     6781 aaagcgttcg ctcgagccat ggacatggcg acgggcttgg accaacgtat cagcggcgtg
     6841 ctgtcgacca agggtagtct ttaagagaaa cggcagtggt tgagtgttat agatcgtcag
     6901 atctgtaaag cagcagagta gattcatgat tgcgattatc gattacggca tgggcaacct
     6961 gcgcagtgtc cagaaggcgc ttgagcgggt gggctatgag gctatgctca cgcgcgaccc
     7021 cgggcagatc gagtccgctc gcggcgtggt cttgcccggg gttggcgctt tcagcgcctg
     7081 tatggagaat ctcagccggt ttggtctgat acagcctatt tgcgatatca tcgggcgtaa
     7141 aaagccgttt ttgggtattt gtttgggatt tcaattgctg ttttcagaaa gcgaagaatt
     7201 cggcagacag aaggggctcg acttgtttgc cggcaaggtc gtcggctttc attctacgga
     7261 cggcctaaag gtgccgcaca tgggttggaa ccgtctcgtt aagaaaaaag attcaccctt
     7321 tttggaggga gttcccaacg gtgaattcgt ttactttgtg cactccttct atgtcgttcc
     7381 ggaagacgct tcggtaatag ccaccgacac tgaatatggc agtccgtttg tttccagtat
     7441 cgctaccgat tcgctttttg cctgccaatt tcatccggag aagagtcagg aaatcggcct
     7501 gcgtattctt tcgaacttcg gccgcttcgt cgcttccaat tgactcatga tcattattcc
     7561 ggcgatcgat cttaaacatg gccgttgtgt tcgtttgttc cagggcaaaa tggatcaaga
     7621 aacggtctac tttgaagatc cgttggaagc tgcgcggcat tggattcgcg agggcgcgac
     7681 aatcattcac atcgtcgact tggatggcgc ggtcgaaggg cggccggttc atacgaagca
     7741 ggttgaagca atatgccggc gggctagcct ggcagtggaa ttcggcgggg ggcttagatc
     7801 catggaagct gtcgaagcag ccttcgagct cggggtttct cgagtggtga tcgggactgc
     7861 agcttacgat gaccccggct tacttcgctt gctctgtgaa aagtttcccg gccgaattgt
     7921 tgtcggtctc gatgcgcgcc aaggcaaagt cgcggtgaaa ggctggaagg aaacgacgcc
     7981 gatgaatgcc gtcgagctag cccagcgctg tgaacaggac ggcgcctcgc gcatcatcta
     8041 cacagatatc agcaaggacg gaacagggga aggtgtcaat gccgatgaga ccctgaagat
     8101 tgcgcgggcg gtaaccattc cggtaatcgc ctcgggtggc gtagcgagct tgcaagatct
     8161 cagaaagctg gcaccgctcg aaagtgaagg agtggaaggg gtcgtgatcg ggagggcttt
     8221 gtatgctcga gctttcaccc tgcttgaggc caaaagcatt tttgcttgat tcttgactac
     8281 cgaacgggtc agtacttttt tctgcttttg agcattttcc gtgtcatctt tgtcattagc
     8341 cgggtctgac actttgaaaa atctacgcgc ggggatcctt ctctgcttga cgtgcgctga
     8401 agaggaagcc aaaatggcag ccatagaaag gtgtattgct atgaaagata aaaagtactc
     8461 agtattgatc gccgtgattc tgctacttag ctttgccgcc atgccgggca tgagcatagc
     8521 gcaggaggcc aacgagcgaa gtaccggacc ggggttcttt agcgtcctcg gcagcattgt
     8581 tttcaccacg ttgcatgtgc catttaaggc agtgacgtgt gccgggactc aggtgacctc
     8641 cgctgttgcc tacgccggta ccttcggcgt ggaggggcat tatgacggcg gtaccaacgg
     8701 aagagacatc ggcgaaacgg cccgacgctc ctgtactggg tcctggatcg taagaccctc
     8761 ccaggttgca aaagactacg gggaataatt tgtagttcgc cccccatctc ctgttcatgc
     8821 ttccagacga gatgccgcaa tcggcatctc gtcttttttt ggatcaaacc gttacgatcg
     8881 tcggcaattc atctgctgca aacacttcgt attcgtaatt tgattgttta ccacagcctg
     8941 aacgcaatcg atcgtatatc tcgcaaatcg tgccgatatc ggagttaatc cctaatccat
     9001 ctacataatc atcctgctga gatccacgtt catccatccg tagctattta gcaggttggg
     9061 aactggggac agtctttcct gaaggctgtg attgtaagcg cgcggctagt tcacgaagat
     9121 ttttgtacat cgatgctact tgttcgccta taataggccg gaacgagaat ggagacgata
     9181 ccacctgtaa gtttcgaggg attctacttc tcagttgcat cttctgccgc atgggctccg
     9241 cagatcgaga cccgggatgc gtggctggct tgggcttttg atcatgaagt cgccgcaggt
     9301 agatcaactc catctctgga agcaatgccg tcgatactaa ggcggcgagc tggttttctc
     9361 ggaaagatgg cgctggaggt tgcttaccga tgtctcggtg cccgaactga tattccgacg
     9421 atattcagtt cccgacatgg ggaggccgcg cgctcggctg atctgctctt ggatttggcc
     9481 gacgggacgg cgctttctcc cacctcgttt agtttgtcgg ttcacaatgc ggccgggggc
     9541 cttttttcca tagcgcgcgg cgaccgagct tcgaacatgg ctctcgcggc gggcgtcagc
     9601 actgtcgaac atggcgtcat tgaagcttgc ggattgatct ccgagggcgc acctgccgtt
     9661 ctattggttg tttacgattg ccctctacca gccttgtatg ctgacttcga agattgtgat
     9721 gagcaaccct ttgcctgggc atggttaata gagtcggcag ctggcgaggt gttgtcgctt
     9781 cgttgcgagc ctggttccgg cgtgggtcca tcgcgggcta aaggtatgcc agccggatta
     9841 gagattctac gttttttcct gcgcaaggat tcggccttag atcgggtttg caataagcgc
     9901 cggtggcttt ggtcccgtca tgttcagtag gatcggccgg tggtggcgtg tcttcggcac
     9961 tggcgtgagt ttcgtcgcag taggagttgg cggtgtgttc ttttttccgg tgctcaacat
    10021 cgttattcat caaccgcaac tgcgttcgat gctggcccgt cgcgtgatta gttttagttt
    10081 ccgctgcatc ctaggattaa tgtggatcat gggtgtcttt cgttacgaga tcaccggaat
    10141 ggaacggttg aagcggaagg ggctactaat tctcgccaat catccgacgc tcatcgatat
    10201 tgtgtttttg atggctttcg tcaagcgcgc ggactgcatc gtcaagagcg gcctttggcg
    10261 taatccgttc acgcattcga ctattcgtgc cgctgcgtac gtccgcaacg atgatcacgg
    10321 cgtccagctt attgaagatt gtgtgggagc gattcgacgt ggcggcaatt taatcgtttt
    10381 tcctgagggt acgcgcacac ccagcaatgg gtccatcgta ctcaaacgag gcgccgccaa
    10441 tatcgccgta cgaggcttgt gtaatatcac tccagtgctt atacgatgcg cgccgccgat
    10501 gttggccaag ggtgaaaggt ggtggcggtt accgtttcgt ccatcgcatt tcaccatcac
    10561 agtcaaagaa gacatagaag taaagccctt tatcgctcgt gccggtagtg agcctttggc
    10621 catccgtcat ttaaccgatc atctgcagga gtattttgcg aaggaacagc cgcccgatgc
    10681 aatcggatga tgatgaaatc aaacaaatga ttatagacgt tcttcaactc gaagatgtta
    10741 cgcccgccga tattgacaca gatgcccctt tatttgtcga gggactcggg ttggattcta
    10801 ttgatgcctt ggaactcggg gttgccattc acaagcgtta cggggtcaca ttgtcggccg
    10861 attcggatga cactcgccgc catttcgcgt cggtgcgcgc acttggcgtg atgatcgata
    10921 agcaccgtag aaaatgaggg aacggatatg actccggcaa gcccagtcag cgaggaagag
    10981 atctaccctt ggttaatcga tttattacac gacatgttcg acctggataa ggcgaagatc
    11041 acctgtgaag cgaatttgta cacggatctt gacatcgata gcatcgatgc tgtcgatctc
    11101 gccgtcaagc tcaagcaact taccggcaag cggctgcagc cggaagtttt taaaaacgtt
    11161 cgcacggttc aggacgttgt aaacgcactc gccggactgc tcctgaagta gcaggtgcgt
    11221 cggttcttag ccctgtgggc cccatcgatc atgattctct acccgttcgc cgtctggcta
    11281 ggtcatggga aggtcgagcc ccggtttttt gccgggctgc tactaattgc cggtttcatg
    11341 agattgcctc tgatgaaagc cggtgccgcc ggaaccggct ggttggcggg gacactgtta
    11401 ctttctctga ttgctttttg gggcaatcat gttctgacgc tcaaacttta tcctgtcttg
    11461 gtgaacggag tgatgttagc cgttttcacg tatagccttt ttattcctcc atcgatcatc
    11521 gagcagttcg cgcgcaggcg ggaaccgaac tttgggccgc ggagcgtccg ctatacacgg
    11581 cgtgtcacac aggtctggtg cgtcttcttt gctctcaacg gcgcggtcgc gcttatcact
    11641 gcgctatgga cctctgcagc gacctggtca ctctacaacg gggttatagc ttatctgctg
    11701 atgggagtcc tctttgccgg cgaatacggg gctcgtcgta acttcaagcg ccgactcaat
    11761 gcctgaaact ctagacatgt tgagcgttgc cgtcatgccg cgtccgctca gtcaaaccat
    11821 cgcctggcga gatcagcagc ccgtaagcta tggagaattc tttcatcgca ctcgcgcatg
    11881 gcagcagttg ttgagtcgaa tctcggggca aacgttcgct ctttctctta gcgatgcggg
    11941 tgagtttgct gccgctcttt tcggcgcttg gcaggcagga aagatcatat acctgccagg
    12001 tgatcgcctg ccggggacct gtcatgcgat gcgcagtctt gtcgacggtt acttgggcga
    12061 attcgcgccc gaatggaacc cccggacgcc gacacccgag gacggattgg caccaggcgc
    12121 tgaattgaag cctttggacc caaattttgt cgggttattt atctatactt ccgggagctc
    12181 gggcgcggcg caagccatat cgaaaaaact gtcacagatg tcggccgagg tcgcaactct
    12241 agaagcccag ttcggtgaca tcatcgatga ctccgatatt attgcgaccg tttcacatca
    12301 gcatatctat ggactgctgt tcaatgttct gtggccgctt agcgccgggc gcccgtttgc
    12361 cgcccgaagt tttggttcgt tagagggttt ggcgatggct cttgcccgac gcgatgctgt
    12421 tttggtttca accccggctc atttgcgaag actaccggaa actccggact gggagcttgc
    12481 ttcaaaacgg gttcgtgcag tcttttcatc cggcggtccg ttgccggcgg atgccgctcg
    12541 cgagagttcc caattacttg ggcaggtgcc gattgaggtt tacggcagtt ccgagaccgg
    12601 cggcatcgct tggcgtcagc aaaagactcg acatgatgcg gaatggacac ctctgccaaa
    12661 cataaaatgg cgcatcgacg ccgaggcagg cgtcctcgaa attcgttcgg cgcatttacc
    12721 ggatgcagag tggtttcgaa ccgccgatcg ggcgagaccc gcgggaggca accgtttcct
    12781 tcttgaaggg cgtgttgatc gagttgccaa agtcgaaggc aagcggattt ctttggacgc
    12841 cattgaagag cgactaaagg catcgccgct ggcgcgcgat gccagagccc tggttattga
    12901 agggcgccgg caacgaattg ccgctgtggt cgtcccgtcc gcaaccggat ggcacaagtt
    12961 agcgcaccgc ggcaaactgg ctgtcaatcg catgttgcgc ggtctgcttc gagagacgat
    13021 tgagcccgcg ggcttgccac ggctttggcg ttacgtcgat gctctgccga tgaacacgca
    13081 gggcaagacc actcatgccg cgctcatcga cttatttgac aatcgggata atcgtccaag
    13141 acaagccatc gaatgccggg tccataaagt cgatcagcac gccatctttg aactgatcgc
    13201 accaagtaat ttgctttatt tcgatggcca ttttcgtgac gtgccgattt tggcaggtgt
    13261 ggttcagatc gaatgggtcg ttgcctttgg ccggcgctgc tttgatttac cacctttgtt
    13321 tcgcgccata cacgcgctga aattccaacg ggtgattccg ccggagttgc cgatcaccct
    13381 cgaactggcg catgacccat tgaaatcctc tttggctttt aaaatcagct ctcatctcgg
    13441 ggcccatgcc agcggtcgaa ttatcttcag agcggccgat gtttaagcca tgcgtcgtga
    13501 tccccgtgtt cgatcacgag cgcgcagtgg gtgcggtggt cacggaagtg ctggctcaga
    13561 acttagcatg cgtgctcgtg gacgacggca gttcgagtag atgttccagc aggctgcacg
    13621 agatggccgc ggctcgccct cagatgatta ccttgcttcg ccatccaatg aaccgtggca
    13681 agggctgtgc cgtgctcact ggactgcgat atgctgctga ggcgggttat acgcacgccg
    13741 tgcaagtcga tgccgatggg cagcaccgga ttgcggacat tcctcgtttt ctcgagcagg
    13801 ccttggcgca tcctaacgcg ctgatcgtcg ggcacccgga ttacgatagc acggtgccgg
    13861 cgttgcggct ctatgctcgt cagttgaccc acatctgggt atccatcaat accttatctc
    13921 gacagatcaa agattcgatg tgcggcttcc gtgtctatcc tctggcgccg gtgatcgcgc
    13981 tcgaccagcg gatcaacttg ggcgcccgca tggacttcga tacggaagtg ctggtgcggc
    14041 tatattggga tggtctgcat attctcaatg tctccacgcc cgtgacttat ccggcggatg
    14101 gaatttcgca tttccgtggt tgcctcgata acgcgctgct ctcgcgcatg cacgcaacct
    14161 tgttttttgg aatgctgctg cgtctgcctg tcttgattgc acgtaaatgc cgccgccaat
    14221 gatccaagtc atcgctgcta gacgtatacg gcactgggct cagatcaatg aatgcagctt
    14281 cgtcgctgga atttatctgc tgttctggat tacccgcgtt ttcggacggt ggccactgcg
    14341 ggctgcgctt tatccggtgg tgctgtggta catgatgacc cgacctgcgg cccgggctgc
    14401 atccagagac tacttacgcc gattggcgga ctcgggaggg caagtaattc tcaaacccga
    14461 tgtcttcagc gtcttccggc attttgcctg ctttgccgaa agtattctcg acaagatgct
    14521 tctgtggagc ggcctgtaca aaactgatca ggttaaattc gtgggtaagg agctgatcat
    14581 ggcgcaaacc gccgcgcaga gcggtggttt gctgatttgt tgtcacttgg gaaattcgga
    14641 attaggccgt tttctttcca aacagctgcc gggtttcaaa ttgaccgtat tgatccatac
    14701 caaacacgca cagcgattca atcgcctctt ggcgcaactc gatcccgaga gtcaggtcga
    14761 ccttatgcag gtcacggaac tatcgccggc taccgcgatt gtgctcgcgg agaaattggc
    14821 ccagggcgaa tttgtcgcca tagcgggtga ccgtattccg gtatcggcca atccgcgcgt
    14881 tgccttagcc gagtttctcg gaaccgtggc gccatttcca gcgggaccgt acattcttgc
    14941 aagcttgttc cactgtccgg tctacttgat gttttgcagg agttacggag gcacggcgga
    15001 aattcacttc gaactattcc atcaggtgat tgatttgccg cgaattggcc gtgatcaggc
    15061 gctcgctcga gtagctggag agtatgctcg acgtcttgaa catttctgcc ggcgcgcgcc
    15121 gctgcaatgg ttcaacttct acgatttctg gagcctgtcg tcggtggaaa attgagatgg
    15181 agagccctgc attcaagaaa acgacccgga ctgtacgatt cgatagggag tgggtgaaga
    15241 tccaagatat cgtcgacgtt gccgacggtt tagcctggcc cgtattgtcc gagacgccac
    15301 agttccgctc cgccattgcc caaggcgcgg agtttgtcga ccgcctcctg cgtgaagacg
    15361 gcaggatcta cggagtgacg acgggttttg gcgactcgtg catggtgact gttgggacgg
    15421 agctcgccgc cgagttgccg cgccaccttt atacttatca cggttgtggg ctcggggagt
    15481 atctcacgcc ggcacagact cgcgcggtca ttgcaacccg cctgacttct ttatgcaaag
    15541 ggttctccgg ggtcagtgtc gaactgttgg agcaaatggt gcgcttgctg cattgcggtg
    15601 tgttgccgag aataccatcc gaaggctcag tcggcgccag cggtgatctt acgccgctct
    15661 cttatctcgc cgccgtgctc tgcggcgagg gcgaagtgtg gcgcgaaggc gcagcggcac
    15721 ccgccgcagt ggctttggct cgtgcccgaa tcccgccgtt aagtctgcgg ccgaaggaag
    15781 ctctcgcgat catgaatggc accgcagtca tgacggcctt ggcatgtctc gcttactcgc
    15841 gcgccgagta tttaagccgg ctggtgacgc gggtgacggc gctggcctcg tttgctttag
    15901 acggcaatgc ctatcacttc gatgagacgc tattctcagt caaacctcat ccggggcagc
    15961 agcgggtagc cgcttggctg cgcgccgatt tgctctgcga tcaaccgccg cgcaacgcca
    16021 cgcgccttca agaccgttat tcgattcgct gcgcgcctca tgttatcggc gtgcttgccg
    16081 atgccttacc gtggctgcgc gagtctatcg agaacgagtt gaacagcgcc aatgacaatc
    16141 cgattatcga tgcggcaggt gaacgggttt tgtacggcgg tcacttttat ggcggccaca
    16201 tcgccttcgc gatggatagc atgaaaaacg ccgttgccaa tctcgctgat cttctcgatc
    16261 gacaaatggc gcttttggta gacagccgtt tcagcaatgg cttacccgcc aatctatccg
    16321 gcattgaggg cccgcggcag gcgattaatc atgggcttaa agcgctgcag atcagcgctt
    16381 cggcatggac cgccgaagcg ctaaagctca ccatgcccgc ttcggtcttt tcgcgttcca
    16441 ctgaatgcca caatcaggac aaggtcagca tggggacgat tgcagcgcgc gattgcctac
    16501 gcgttttgga attgaccgag caagttgtcg ccgccctgtt gatcacagtc cgacaagggg
    16561 tttggctgcg ctgcagatcg aatcgctctt tgagattgca agcggcgtta cagacgatga
    16621 tgcatcagtt ggagaacgat gtcgtcgccc ttgaggaaga ccgacggctc gaaccggatt
    16681 tgcgattact tttggaacgc gtccgcagcc gcgcgtggga actctatgag taagccgtcg
    16741 agccgctgga tcgctgaaac cgagacgcag atagaattct tcgatctcga tcccatggaa
    16801 atcgtttggc atggtcgcta tgtcaaatac ctggagatgg ttcgcggcgt gttgctcgat
    16861 accatcgatt acaattaccc gcagatgaag gcgtcgggct atgtttggcc ggtgatcgac
    16921 atgcacctgc gttacatcgc tccggcgatt ttcggtcaac gaattaagct gcgggcagag
    16981 atcgtcgagt gggaaaaccg ccttaagatt ggctatctca tcaccgacgc gcagagcgga
    17041 cgccggctta ctcgcgccag caccacgcaa gtcgccgtcg agatcgccac ccgtgagatg
    17101 tgtttcgtat cgccgcgggt gcttctcgaa agaatgggag ttgggccttg ttaaggcggc
    17161 agctggtcgt attctggctt gtcctgtccg cgccgccgct cctcggcgcg acgcccctat
    17221 cgaccatcga gtcgatgctg ctcaaaccga ccgtattgtg cggccgtttt gagcagaaaa
    17281 aacagctagt tgggttcaag aatgccgtga actctagcgg ccggttctgt gtggttgccg
    17341 gtaagggtgt gttgtggcgc acccttaagc cgtttcctac tgccttgcgc ttaaaccgtg
    17401 atgagatcgt ccaactgcac ggcgatcggg tgacgttgcg tctcgaggcc aagaaagagc
    17461 ccactgttcg aatgatcaat accgttttgt ttgccttatt agcggtcgat atcgggcagc
    17521 ttgaaaaaca cttcgagatc gacggctcag ttcaaaacaa cgtctggagc ataaggctga
    17581 aagcgcttga accggctttg gccaaagcga tcggcagcat cgcgttcgaa ggcggcgtct
    17641 atgtcagaag tatacgaatc gatgaagcga gcggcgatcg cacgagcatc gatttgatgg
    17701 caatcgaaac cggcgaaagc gccatggatg ccgatgaggc gaagctgttt tgattcgcct
    17761 aaaattactg gcttggttct ggagcctggt ggtgtgcttg ctcgtagtcc ataactcgta
    17821 cctctggctg caccaacgac ttgccccaga gaccgatatt ctggcgctgc tgccgcttca
    17881 acaacgcgat cctgtactac agcaagcttt cacccgaatg gttgatacag gccagcagcg
    17941 gctgattgta ctcatcggcg ccgatgactg gtcgcaagcg cgagatgccg ccgacgcgta
    18001 ccactcggag ttgttggcat ataaggatct gctgcaacta gagcaccgcg cgacttttga
    18061 ttcgcaacaa gagtggctcg gattgttcga gcaacatcga ctaggcttgc tgacacgcga
    18121 gcaagaatcg gcattacatc aagaacccgc gcagttttgg atagatcgcg cattgagcaa
    18181 gctgtatcgg ccgtttggcg gctcgatgct aacggcttgg cacgatgatc cattcaatct
    18241 ctttggcgaa tgggcgcagg cgcgcgccca agaaacggcg gtgcggccac gcgaaggtcg
    18301 gttgtctatc agcgagggcc aacgcgaata tgtcgttatg ccgttcacgt tgcgagttgc
    18361 ggctttttcc atcgccgccc agcagtcgat tattccgcta ctgcagagag ccaagaacaa
    18421 cgctcgcaag gcggttccgg gcgttgaggt tctatcggca ggtttgatcc tttatgcgga
    18481 cgctgcagcc gagcagtcga agctagaagt cggcaccatc ggactcggtt cgatgctcgg
    18541 catcattgtg atgacctgga tcgcatttca ctcgctaaag ccgatagctt gggtgatcgt
    18601 ctcagtggtg atcggttgcc taggcgcttt atcgatttgc tgggcggttt tcgatcgcat
    18661 ccacttactg accctggtat tcggcgccag tttgatcggc ggcgcccagg attatggaac
    18721 ctattttctg tgcaaccgat tcgccgcgga tgcgcgccaa ctgaactcgt ggcaattact
    18781 gcgtcggctc atgccggcgc tggttttggc gctggtaacg accgtgattg gctatctcgg
    18841 ccttgcgttg acgccatttc cgggtttgca acaggtggcg gtattttccg cgataggctt
    18901 ggtattcgca tggcttactg tgatctgttg gtttccgact ctggtctggc gaggcacatt
    18961 aaagggcggg cgttttgccg aatactacgc taccactatg cagcagtggc cagtgctgcg
    19021 ttggaatcaa aggacggtgg cggttggttt agtctcgatc gcttttgcgt cctgcggctt
    19081 ctcggcgctt catacagagg atgacatccg ctcgctgcaa aatccgccca aacacctcgt
    19141 tgacgaacag atcaagctcg ggcgactttt ggacgcgccg gcgccggctc aattctattt
    19201 gattcgaggg cccaccgccg aggcggtgct ccagcgcgaa gaagcgctca cgaagcggct
    19261 tgagccgatg atcgagaaac gtctgatcac tggctatcag gcggtatcca attgggtccc
    19321 ttctctcgcc gcccaactcg agcgccggca acttattcag gagaagcttc tcgatgacag
    19381 cggcccgctc gacatgctgg cgcaaaaact tggagaaggc ggcaaatggg ggtccgggtc
    19441 ccgccaaggc ctggttgcat ccgcattgcc tctttcgccc gacgaattct tcaaatcgcc
    19501 gatcagtgaa cccattcgtc ctatttggct cggcaacgtg aacggcacct atgccagcat
    19561 tgtcgttttg cggggagtga aaaaagatac cttggcaact ctgcagcatg ccgccggcag
    19621 actcgaagga gttcagtggg tggacaaagt tagtgaagtt tcgtccgttc tagcggcata
    19681 ccgccattac atgagttggc tcatcgtatt ttcttatttt gccgtttacg gcctgcttta
    19741 tccgcgctat ggccgcgcca cctggcgggt agtcgcccca acggcgatcg caagtctggc
    19801 tacgcttgcg cttctgggca gtgccggagc gggtgtgcag ttgtttcatg tgcttgcgct
    19861 gatgctcctg ctgggcatcg gcatcgatta cgggattttt ttgcaagagc atttcggtcg
    19921 tcgcgatgcg attgcatggc tatcggtggc gctctcggca atcaacacct tgctctcgtt
    19981 cggcctgctc agtttaagca agaccccggc tctgcaagct ttcggcctaa ccatgttaat
    20041 cggaacagtc attttgtgtc tgatcgtccc ctgttttgtt gttggaatca cagacacccg
    20101 ggtcttgacc gagcggtttg gggccagcgc atgagggtga ccggattgcc gcagaaggtt
    20161 gggaaatcgg tggagatcct catcattggc gctggtccag cggggtcagt agcggcgtcg
    20221 ttgctgcgac agcagggccg gcaagtcttg atgctcgagc gcgagcagtt tccgcgtttc
    20281 agcatcggtg agagtttgct gccgcagagc atggaatacc tcgagcgctg cggtatgctg
    20341 cgcgccgtcg ttgaggcggg ttttcaattg aagaacggcg ccgccttcgc tcagggcgag
    20401 caatataccg aattcgagtt cggcgaaaag tattcaacag gctggaacac tacttaccag
    20461 gtaccgcgcg cgcgttttga tcatctactg gcgttagagg cagaggctca gggcgcagag
    20521 gtcagatacc ggcacgaagt aatcggtatc gatttaggcg gtagccggcc ccggttgacg
    20581 attaaggatt cagatggcga gcattatctt gtcgacgcga gctttgtgct ggacgccagc
    20641 ggtttcgggc gtattctgcc gcgtctactg caactagaaa caccgtccaa ttttcccgtc
    20701 cgtggcgccg tcttcaccca tattgaagac cggatcacgc acccagggtt tgaccgcaat
    20761 aagattcgcg tgacggttca cccggaacgt cccggcgtgt ggttctggct tattccgttt
    20821 gccgcagggc gctgttctct gggggttgtg actgaacctg ctttgctcga ccgttatcaa
    20881 ggaccggata cacaacgatt gcaggccttg gtcggcgaag accaaacctt gtccaatctg
    20941 cttgaaaatg cctgttggga tactccggcg cgacgggtcg ttggttatgc ggccaacgtc
    21001 aaatcgttgt ccggtcgaga ctatgccctg ctcggtaatg ctgcggagtt tctcgatccg
    21061 attttttcgt ccggggtcac gatcgctttt aaatcggcga gtcttgccgc cgcggtcctg
    21121 cagcgtcgct tcgccggaga gacgatcgat tggcttagtg aatacgctgt gccgctcaaa
    21181 ataggcgtcg ataccttccg agcctttgtc gactcttggt atcgcggagg ttttcagcgg
    21241 atcattttcc atcgcaatca accgccggaa attcgccgta tgatttcggc gatcctggcc
    21301 ggctatgctt gggatgagag caatccgtat gtgcgcgagc cgagacggcg cttggcagcg
    21361 ttggaggagt tgtgttcgta gcgctgcccg gccggcaggt cgccctagtc ggatcattga
    21421 cgttgttgtt aaccggctgt gtcgcgccct cgccgagggc cgcaggtgtg ggcctgaagc
    21481 tggctccggc cgcactggcc gaatcgatca gtttgcagca gcgattagat atagaagggc
    21541 agggtcggag ggcccagtta gacgtcgtcc tcgaagtcga cgctgaacgg atcgatttgg
    21601 tcggcctcgc gtttggtcag agagttttgt ccatgtccta cgacggcaag acgctacagt
    21661 cgtggcgcca tccgatgtgg ccggctcaag tgcgcgcaga agaggtgctt gaaaacatac
    21721 aactgacgct ttggccggtt gatgcgattc ggcgagctct accgatgggc tggagcatca
    21781 aagaaagcgg tttgcgccgt accctcttac aggatggttt agccgtcatg gtgatcgatt
    21841 acagcgcgtt gcccttttgg agcggcaaag tggaagtggc gaacctgcag tttaattacc
    21901 gcataaccat ccaatcggtt tctaatgagc cttaaatgat ttacctgaac caacttggaa
    21961 tcgtctgccc actgggaagt tcccgtgaag aggtgaaaca tcggctgctg gatcgggtgg
    22021 caagcggggt ctcgctgacc gaaaactatt cgcctggacg cctactgccg ctaggttgca
    22081 tcgatgccga actgcggctc ccgtcagtcg accatttgga cctcatcgca aggagccgca
    22141 acaatcaagt ggcattggct gcattggcgc aaatccgaac tgcggtggac agcgccattg
    22201 aacagtacgg caaaggccgt ttcgggattg tcatcggcac cagcacttcc ggaatcgccg
    22261 aaagcgaagc cgcgtttcgt cactacgtgt ggaatagaag cctgccggag cagttccatt
    22321 atgggcagca ggagttgaac tcgctggcta ctctgctcgc tgatgagctt gacgtatggg
    22381 ggccggcata cgtccattcg agtgcctgcg cctcaagtgc caaggcccta gccagcgccg
    22441 ctcgcttgat tcgcacgggg gcttgtgatg cggttttgac cggcggcgtg gatactctat
    22501 gcggttttac ggttgccggt tttgccgcgc tcgaatccgt cagtgcgacc cagtgcaatc
    22561 ccctcagtcg caatcgtcgc ggcatcaata tcggcgaagg cggcgcgcta ttcttaatga
    22621 gccgtgagcc tgcttcggtc gctctgcgcg gctggggcga atcgtctgac ggctatcata
    22681 tgtcagcacc cgatcccagc ggcgcaggag cgcgtctcgc catcgaacga gctctttcac
    22741 gcgccgaaat ttctgctgag tcgatcgact atataaacct tcatggcacg gggaccatcc
    22801 agaacgacgc catggaagcc cgtgtgatcc atgcgctctt cggcgctgcg gttgctgtta
    22861 gctcgacgaa accattgacc gggcatgcgc tcggcgcggc cggagccatt gaggctggat
    22921 tgtgttggct ggcgatgcag gatgataacc ctgccggcag atttccgccg catctctggg
    22981 atggcatacc cgatccgacc ctgcc
//
LOCUS       DASUVS010000152        11778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_278993, whole genome shotgun sequence.
ACCESSION   DASUVS010000152 DASUVS010000000
VERSION     DASUVS010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11778
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278993"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(407..670)
                     /locus_tag="VF182_08555"
     CDS             complement(407..670)
                     /locus_tag="VF182_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437169.1"
                     /translation="MDQKERIEIGSQVYLEEGGDPCGAVRDVRPGSRNEITIYVENSG
                     EFFVPLNVVRAAHDGNVILDRQRLDESLLDALAHAHDGEVPGL"
     gene            1028..1846
                     /locus_tag="VF182_08560"
     CDS             1028..1846
                     /locus_tag="VF182_08560"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6437170.1"
                     /translation="MNSSYFARLLILSIWVSLAGASGAAHAQPAKSYQPQVGQEGKDV
                     VWVPTPQALVDKMLDMAKLTPQDYLIDLGSGDGRTVITAAKRGAKALGIEYNPDMVEL
                     AKRNAAKEGVSDRAAFTKADLFESDFSQAQVITMFLLSSINMKLRPKILDLKPGTRIV
                     SNTFDMGEWKPDETGRADNCNGYCTAYLWIVPAKVDGTWKLPNGELNVKQTFQMISGT
                     LKSGNVVTPINGKLNGEQITFTAGEGEYMGRVNGNSMEGSLKSGGKWSASHSGK"
     gene            2162..2974
                     /locus_tag="VF182_08565"
     CDS             2162..2974
                     /locus_tag="VF182_08565"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6437171.1"
                     /translation="MNSSRLVSRFLFVLSLSLASATAFAQPAKQYEPQVGQEGKDVVW
                     VPTPQALVDKMLDMAKVTPKDYVIDLGSGDGRTVITAAKRGAKALGIEYNPDMVELAK
                     RNAVKEGVSDRATFTKADLFESDFSQAQVITMFLLPSINLKLRPKILDLKPGTRIVSN
                     SFDMEEWKPDETANAEGCSNWCTAYLWIVPAKVNGTWKLPNGELNVKQTFQVISGTLK
                     SGNVVTPINGKLNGEQITFTAGEGEYTGRVNGNSMEGSLKSGGKWSATRAGK"
     gene            complement(3220..3606)
                     /locus_tag="VF182_08570"
     CDS             complement(3220..3606)
                     /locus_tag="VF182_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437172.1"
                     /translation="MSVNLQAAVMENINELKVAISEKTLEMAELEKELQRYESVLALL
                     RDDHNGNERRKGRPRGRTDLAEVLGGLPETFTSKDFIKAATRTKKPSVYLRLALSRWA
                     KEGRVKRLERGKYQKVRNGSSHRLAA"
     gene            complement(3886..4911)
                     /locus_tag="VF182_08575"
     CDS             complement(3886..4911)
                     /locus_tag="VF182_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX6437173.1"
                     /translation="MGKYHVVYFGSNDVPVDLIKPILAEIDAEMTIRRPTNDAEVVEM
                     GKDADGIIMHGSVPLSREIIAQLKRCKVVCRTGVGVDRMDLKAASDQGMIICNAAGCN
                     SIEVSEQTIGLLIAISRKLVRMNQYVHDGKWRRHTAELHAYRGKVYRIQGRTLGIVGL
                     GHVGRQVAPRAQGMKLNVQAVDPYLDSKVAQSLGVKLVSFDELVATSDFISLHAPLTK
                     QTRKMFSAPQFKAMKKTAYLINCARGGLVDTEGLYEALVAGELAGAALDVTEPEPLPA
                     DHKILTLPNVIVTCHTAANSDESYIDCQTHAAREVVNVLSGRGPTTEVKDPWLLAEAQ
                     DEGFGGV"
     gene            complement(4950..6053)
                     /locus_tag="VF182_08580"
     CDS             complement(4950..6053)
                     /locus_tag="VF182_08580"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6437174.1"
                     /translation="MYIDGDTHYWPVRFIERVDHPRRGHVELKKGAGEMIRYGEKFPG
                     DAATYYRDGKKIHSFSETRWNLQIRHDVMEREGFDYQVVIPDNRPLIYEVEPSLGNAM
                     ARAYNDTVAEDTGDSDRFIPISWVYLPDVDEAVRELRRSVTELGFKGVKLMGGHQNCD
                     LDAEVLWPFYEEVCRLDVPVLVHPGTRIFEAQDCHPWLVGSERYDGFKMLATALGFPL
                     TYMVSAARLIMSGTLDRFPNLRFAFFEGGIGWVPWLMKTLNHHTLSAAPGTTSVNDFF
                     RGRDRLKKEPSAYFEQLYVAAIAWEDYLPKIVEIWPDHNIIIGSDFDHGDAISTWPNT
                     VEPIKAMSGLSDRDKDKILGGNAMKLFGMKKAH"
     gene            6225..7175
                     /locus_tag="VF182_08585"
     CDS             6225..7175
                     /locus_tag="VF182_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-dihydroxyphthalate decarboxylase"
                     /protein_id="HEX6437175.1"
                     /translation="MAKTKITLALGSYDRHGPLLEGCIQHPEFDVQYIELDPQQGRHE
                     RFLQNSEFDAAELSLSSYLIAIDRGMPVHAVPIFPRRLFSQSQMYKNVDCGIDSLMDL
                     AGKRIALSGYQNTLGVRAKGDLNHFYGVPQTSITWITPGKEVLDLELPGDVKLETRDS
                     MGQIEAEFVQGKIHAMFVSRLPKVFREGNPNVARLFSDPQAEEERYLEAEGYFPIMHV
                     LAFRKQLAQRYPELPGALYNLFEQARRHAAQRWADPNWSIVIWGRRELERQETLCNFD
                     PWRNGLEANRKNVERFALYSYEQGLTKRQLRPEELFVAIQ"
     gene            complement(7369..8214)
                     /locus_tag="VF182_08590"
     CDS             complement(7369..8214)
                     /locus_tag="VF182_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX6437176.1"
                     /translation="MRLDQEGQSTNVEDRRGMRMSRGLAGGGIGTIAIVLIGLFFGID
                     PSVLLQGNIDSGPPSQQVPQQGATDEMGQFVSSVLASTERTWGEIFRRNGQEYQEPRL
                     VLFSGAVESACGFAQSASGPFYCPQDRRVYIDLEFFRELRDRFKAPGDFAQAYVIAHE
                     IGHHVQNQLGVMRKTGELQSRSSRAQANAISVRVELQADCLAGIWAGTANRDRKILEE
                     GDIEEGLNAAAQIGDDRIQKRSQGYVVPEGFTHGSAEQRVRWFRRGLESRDLGQCDTF
                     SAGAV"
     gene            complement(8459..9457)
                     /locus_tag="VF182_08595"
     CDS             complement(8459..9457)
                     /locus_tag="VF182_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437177.1"
                     /translation="MRIEKLISSAAAFALVLAAWGFMPRELLAQRIKVGYWTSGFSLG
                     FGAVLEQMKLAEKEGLEVEWVKFAEVNGPTRAIVSNAIDLAFAAPSTNSIGIAADGVP
                     VKIVLATQIAEAQVVVLDGAPIKTAADLKGKKIGMSPPGSATHAISTAILDQNYGIKP
                     KEYSVAPGNEARLAQFLTQREIDAAIIRSVTIAQMEETKLRRLASIVDEWKKLTKSSA
                     APILGVTIVYNDYLTKNADAVAKFIVAMRKTVEYGSKNKTRVAEILQKAANMDNKDAT
                     AYASQWDGAYMASFEPQDITSLKRMHEIVKAHGGAQKDPPESAFDAKPYMRSKQLK"
     gene            complement(9614..10408)
                     /locus_tag="VF182_08600"
     CDS             complement(9614..10408)
                     /locus_tag="VF182_08600"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6437178.1"
                     /translation="MDNPSLAQQACIEPGQPAAAIRLSGISKSFAAGDGRLEVLKDVN
                     LTVADHAIVGLLGASGSGKSTLLNIISGIVKPDHGQVCICGAPSAEFNDWRTISYMFQ
                     DDRLLPWRNAMRNVEFALEAGAMPKSERIRRAGQALELVDLNGFEASFPYQLSGGMRS
                     RVALARSLVTEPRILLMDEPFSRLDAQTRGLMHEELLRIHEIKRMTVVFVTHDVEESV
                     ILADEVAVLSPRPGTIREIVPIALARPRDTTQAEVSNYIQKLRGLI"
     gene            complement(10480..11304)
                     /locus_tag="VF182_08605"
     CDS             complement(10480..11304)
                     /locus_tag="VF182_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6437179.1"
                     /translation="MKDETTEAVADNVWTRSNPRKLRFDLLLQRVVLVALFIGIWWLF
                     SLSVPHYILPGPARVGQALSLIAANGDLWSNLGITFWRVTVGFVIAALVSLPFGIFLG
                     ANKRAGEFFEPVIPVLNTVSSAIWAIFAIIWFGISNATTIFVVFMTAMPLIITNVWQG
                     TRTVNAEFIELAQVLRMPKWKVMTKIYLPTILPYFFSGARLAYGFGWRVSLVAETIGA
                     SSGVGYRLRQAADLIQTDQVFAWTLTLVAMMATLEMGLLKPLENHLFRWKKEAEGQ"
BASE COUNT         2750 a         3262 c         3070 g         2696 t
ORIGIN      
        1 gttgctctat tttgtagcta gcctaacagg ataaagcaac aggaagcgtt gcatggatct
       61 ccataagtca ccaagcctta gaacaggaga accgatgccg ctatcgccga aacacctcta
      121 aagcttctcc caccggcttg acgggcccac tgagctcgcc acgaatcaca agcccgtagg
      181 tagtatagca atgccaacgc agaagttcga tgagaacccg agatagtagg gaagcgcgac
      241 aagctaatgg atggatgaac cgaaatcaat cggacgacaa cagggagctc gccctcaaag
      301 agccgctgag gatggcattt tcgatccgcg ccgcaatcta cccgcggtct ccaacgcctt
      361 gaaacaatct ggtgggctac gtcacgccgc tggtaagggc caccgattaa aggcccggaa
      421 cctcgccgtc atgcgcgtga gctagtgcat cgagcaggct ctcatccaaa cgctgccggt
      481 cgagaatgac gttgccatca tgcgcggcac gaacgacatt caggggcaca aagaactctc
      541 cagaattctc aacatagatt gtgatctcgt tgcgacttcc cggccggaca tcgcgcaccg
      601 cgccgcaggg gtcgccgccc tcctccagat aaacttgcga gccgatttct attctctctt
      661 tctggtccat aacaccatcc ttatttttgg tgcgggtccg gagtcacttg accgagatga
      721 tcgctcgcta caacaggcag gcatcgcaac atggtccaca gtttataacc cggccgaatc
      781 cgctttaatt ttgtttgtac taaatgtcgc aaccttcgta ccattagaat ttcaaaaact
      841 tgccgctgct atagttctat ctcctgcaca ggttcgtgga ttgtgccgta tcgacgacat
      901 tccgtttatc gtttgttgcg acggcaagac attgacgcgt aggtggcaag attataatca
      961 gtattggtta ctaggtctca accctgttcg aacctgctat gctcaaccca actttccggg
     1021 aggatttatg aactcgtcgt atttcgcgcg actattaata ttgtcgatat gggtatccct
     1081 ggcgggcgct tctggagcag cccatgcgca gccggcgaaa agctatcagc cgcaggtcgg
     1141 ccaggaaggc aaggacgtgg tctgggtgcc aacgccccag gctctggtcg acaaaatgct
     1201 cgacatggcc aaactcactc cgcaagatta tctcatcgat ctgggttcag gtgatggccg
     1261 cactgtcatt acggcggcta aacgcggcgc caaagctctt ggaatcgaat acaacccgga
     1321 catggttgag ctcgccaagc gcaacgccgc gaaagaaggc gttagcgatc gagcggcctt
     1381 caccaaggcc gacttgttcg aaagcgattt ctctcaagct caggtaatca ccatgtttct
     1441 cctgtcgagc atcaacatga agttgcgccc taaaattctc gatctcaaac cgggaacgcg
     1501 cattgtctcg aataccttcg atatgggaga atggaaaccg gacgaaaccg gcagggctga
     1561 caattgcaac ggttattgca cggcttatct gtggatcgtg ccggcaaaag tagacggtac
     1621 gtggaagctg cccaatggcg agctgaacgt gaaacagacc tttcagatga tcagcggcac
     1681 tctaaaatcc ggcaacgtcg tcacacccat caacggcaaa ctaaacggcg agcagattac
     1741 tttcactgcc ggtgagggag agtacatggg ccgggttaat ggcaattcca tggaaggatc
     1801 gttgaaatcc ggcggcaaat ggagcgctag tcacagcggc aaatagtgga aggagcgttc
     1861 gcggagtaaa tcacccgtca acgcgccctc aagcacgtgc agtctgttta atgtccccgc
     1921 gttatcacgt cgcaaagaac cgggcctaag tcattgactt gggccttagt ttctccagcg
     1981 gcttcgatcg ataatacaaa atccaagttc ttggggtcac ctggcatcct gaactatcct
     2041 ggcccacctt tctggacaat tgtgcatccc taaggtatgg ggtatggatt gatagtcttc
     2101 ttctgctccg ttcaccggtc ggtagcgcaa atatccactg attattcata gggaggaatc
     2161 catgaactca tcgcgtttgg ttagtagatt tttatttgtg ctaagtctct ctttggctag
     2221 cgccaccgct tttgcgcagc cggcaaaaca atacgagccg caggtcggcc aggaaggcaa
     2281 ggacgtggtc tgggtgccta caccccaggc tctggtcgac aaaatgctcg acatggccaa
     2341 agtcactccg aaagattacg tcatcgatct gggttcaggt gatggccgaa ccgtcattac
     2401 ggcggctaaa cgcggcgcca aagctcttgg aattgaatac aacccggaca tggttgagct
     2461 cgccaagcgc aatgccgtca aagaaggcgt tagcgatcga gctaccttca ccaaggccga
     2521 cttgttcgaa agcgattttt ctcaagccca ggtaatcacc atgtttctgc tgcccagtat
     2581 caatctcaaa cttcgcccca agatccttga tttaaaacct gggacgcgca ttgtatcgaa
     2641 ttcttttgat atggaagaat ggaagccgga tgaaacggcc aatgcggaag gatgcagcaa
     2701 ttggtgcacg gcttacctgt ggatcgtgcc ggcaaaagta aacggtacgt ggaagctgcc
     2761 taatggcgag ctgaacgtga aacagacctt tcaggtgatc agcggcactc taaaatccgg
     2821 caacgtcgtc acacccatca acggcaaact aaacggcgag cagattactt tcactgccgg
     2881 tgaaggagag tacacgggcc gggttaatgg caactcgatg gaaggatcgt tgaaatccgg
     2941 cggcaaatgg agtgctactc gcgccgggaa ataaatgaac gagaaactgg tttccgctaa
     3001 atgaccagct tgcgagttgc ctccaaagat tacagccatc tcggtcgaac agctgtcgat
     3061 cggagtgcca gcttagctgc aagtacagct actacactcg agagagacac ccgggcggag
     3121 cggtatcgct tctaggctcg ttcgtcaggc tccggtcctg gaccggagcc tcatactgca
     3181 ttgccgatcg gaaggagatt gagcaaagat ttggtgcgat caggctgcaa gccgatgcga
     3241 gctgccattt ctcaccttct ggtatttacc tctttccaat cgcttgactc ttccttcctt
     3301 tgcccatcgg gaaagcgcca gtcgaagata gaccgacggc tttttggtgc gggtcgccgc
     3361 tttgatgaaa tctttgctgg taaacgtttc cggaagcccc ccgagcactt cggcgagatc
     3421 tgttcttccg cgtggtcgac cctttcttct ctcgttcccg ttgtgatcat cgcgcagcag
     3481 ggcgaggaca gactcatagc gctgcaactc tttttccaat tcggccatct caagagtctt
     3541 ttcagagatt gccaccttga gttcatttat attttccata actgcggctt gaagatttac
     3601 tgacatgaca cgatacctcc tggctgaaga gtatgtaatc tgccggatca actcaagatg
     3661 tcttcagtat aaaatctcat tgtttgtagt attgattgaa cggtcataat agcgttgcat
     3721 ctcatcgcag ctttgtaacg cgagctacaa ccgtaggcga cgcgccatag atcgatcttc
     3781 gccgtgctgg aacgtaaaat accagagcaa gagacagcga aggcccgata accgggcctt
     3841 cgcatcactt ataacgagga atatgccgga tgattcggat tgacgctaca cgccgccgaa
     3901 gccttcatcc tgcgcttcag ccaacaacca gggatctttt acttcagtgg ttgggcccct
     3961 gccactaaga acattgacga cttcgcgcgc cgcatgtgtc tggcaatcga tgtacgattc
     4021 atcggagttc gcggcggtat gacatgttac gattacattc ggaagggtaa gaatcttgtg
     4081 atccgcaggc aacggctccg gttcggtaac atcaagggcg gctcccgcca gttctccagc
     4141 gaccagggct tcgtacaatc cctccgtgtc gaccagccct ccacgcgcgc aattgatcag
     4201 gtaagcggtc tttttcatgg ctttgaattg gggcgcgctg aacatcttgc gggtttgttt
     4261 ggtgagcggc gcatgaaggc tgatgaaatc tgatgttgca accagctcat cgaacgacac
     4321 cagcttcacg ccgagggact gggcaacttt tgaatccaga tagggatcaa cggcttgtac
     4381 attgagcttc ataccttggg ctcgcggcgc cacttgccgc ccaacatgtc ccagcccaac
     4441 gatgcccaac gtgcggccct gaatgcgata aacctttccg cggtatgcat gcagctcggc
     4501 agtatgccgc cgccattttc cgtcgtgcac atactgattc atacggacga gcttgcgcga
     4561 aatagcgatc agcaatccga tcgtctgttc agaaacttcg atggaattgc agcccgccgc
     4621 attgcagata atcataccct gatcggacgc tgcttttaga tccatccgat caactccgac
     4681 accggtgcgg caaaccactt tgcaacgttt gagttgcgcg atgatctcgc gcgatagcgg
     4741 caccgaaccg tgcataatga tgccatcggc atccttaccc atttcgacta cctcggcatc
     4801 gttggttggc cgcctgatgg tcatctccgc atcgatttcc gccaggatcg gcttgattaa
     4861 atccaccggc acatcgttgc tgccaaaata aaccacatga tatttgccca tgctaaacct
     4921 ccatttgata caatgggatc gacacgctgt tagtgtgcct tcttcatgcc gaacagcttc
     4981 atggcgttac cgccgagaat tttgtcttta tctctatcgc tcaaaccgct catcgctttg
     5041 atcggctcaa ccgtgttggg ccaagtcgag atcgcatcgc catgatcgaa atcgctgccg
     5101 atgatgatgt tgtgatcggg ccagatttcg acgatcttcg gcaggtaatc ttcccaggct
     5161 atagccgcga catatagctg ctcgaagtaa gcgcttggtt cttttttcaa acgatctctg
     5221 cctcggaaaa aatcattcac cgatgtagtc ccgggcgccg ccgacaacgt gtgatgattc
     5281 aacgtcttca tcagccacgg cacccagcca atgccgcctt cgaaaaaggc aaaacgcaag
     5341 ttcgggaatc tgtcgagcgt accgctcatg ataagtcggg ctgcgctcac catataagtc
     5401 agcgggaacc caagcgcagt ggcgagcatt ttgaaaccat catagcgttc gctaccgacc
     5461 agccacggat gacagtcctg agcttcgaag atgcgggttc ctggatgaac tagaaccggg
     5521 acatccagcc ggcagacctc ttcgtagaaa ggccagagca cctcggcgtc aaggtcgcaa
     5581 ttttgatgac cgcccatcaa tttcacgcct ttgaagccga gttccgtcac cgagcgcctc
     5641 agttcacgaa cagcttcatc gacgtcaggc aaatacaccc aggaaatggg gataaaccga
     5701 tcgctatctc cagtatcctc ggcaaccgtg tcgttgtagg cacgcgccat ggcgttgcct
     5761 agggagggct ccacttcata gatgagcggc cggttgtcag gaatgacgac ttggtagtcg
     5821 aagccttcgc gctccatgac atcgtggcgg atctgcagat tccagcgagt ttcgctgaaa
     5881 gaatgaattt tcttgccgtc gcggtagtag gtcgcggcat cgccgggaaa tttctcgccg
     5941 tagcggatca tttccccggc gccctttttg agctcaacgt gcccgcgcct gggatgatcg
     6001 actctttcaa taaagcgaac cggccaatag tgagtatcac catcgatata catcgtcacc
     6061 tcgttagaaa ttttgaatca catggccgga tcttatttgc aagggcatat gaggtcaacc
     6121 ggttcgccac cgactatctt gaacctgatg tatgaatctg ggtgtaaact tgttttgacc
     6181 agagcctttg acagctttac tcgaatttta gtaacggttg gatcatggca aaaacaaaaa
     6241 tcactttggc cttgggttct tacgatcggc acggcccgct gctggaaggt tgtatccagc
     6301 accccgaatt cgatgtgcaa tacatcgagc tcgacccgca gcagggacgg cacgagcgct
     6361 tcctgcaaaa ttctgaattc gatgctgctg aactgtcgct gtcttcctat ctgatcgcca
     6421 tcgaccgggg aatgccggtc catgccgtgc cgatttttcc aaggcgattg ttcagtcagt
     6481 cgcaaatgta taagaacgtt gactgcggca tcgattcgct catggatctg gcgggtaaga
     6541 ggattgctct tagcggctat caaaataccc tgggcgtacg ggcaaaaggc gacctgaatc
     6601 atttttatgg cgtgccgcag acgagcataa cttggataac tccaggtaaa gaggtgctgg
     6661 acctagagtt gccgggcgat gtcaaactcg aaactcgcga cagcatgggt cagatcgaag
     6721 ccgagttcgt ccaaggcaaa atccatgcaa tgtttgtctc ccgtctgccg aaagtttttc
     6781 gcgaaggaaa cccaaatgtc gctcgcttgt tcagtgaccc acaggcggag gaggagcgct
     6841 acttggaggc ggagggttat tttccgatca tgcacgtgct ggcattccgc aaacaattgg
     6901 cgcaacgata ccctgagttg ccaggagcgc tttacaacct tttcgaacag gcgagacgtc
     6961 acgcagcgca gcgctgggcc gatcctaatt ggtcaatagt gatctggggc aggcgcgaac
     7021 tggagcgcca agagacgctt tgcaacttcg atccctggcg caatgggctt gaagcaaatc
     7081 gaaaaaacgt cgagaggttc gccctgtata gctacgagca gggactaacc aagcgccaat
     7141 taaggccgga agaactattc gtggcgattc aatgaagtcg ctgccaatat cacgacacgg
     7201 ttagagcagc ggccgagctt agatagttat ctccttcgaa tcggatgatg aagcagcaac
     7261 gatggaccag cgcgcggtct tttagcgctg gcagaaggag cccggaactc taagaacgtt
     7321 catagacatc aatcactgac ccgttccgct gttgttcgcg ccgcatcact acacggctcc
     7381 ggccgagaat gtatcgcatt ggccgagatc tcgactctcg agaccgcggc gaaaccagcg
     7441 gacccgttgc tccgcgctgc cgtgggtaaa gccctccggc actacgtaac cctgactgcg
     7501 tttttgaatt cgatcatccc cgatctgcgc ggctgcattc agaccctcct cgatgtctcc
     7561 ctcctcgaga atcttccgat cccggttggc cgtgcccgcc cagatcccgg caaggcagtc
     7621 ggcttgaagt tccacccgca ctgaaatggc gttagcttgg gcgcggctcg agcggctctg
     7681 caactcgccc gtctttctca tgacaccaag ttgattttgc acatgatgac cgatctcgtg
     7741 ggcgatgaca taggcctgcg cgaaatctcc gggcgctttg aaacggtcgc gcagctcgcg
     7801 aaagaattcc agatcgatat aaaccctcct gtcctgcggg caataaaatg gtccggaagc
     7861 cgattgagca aacccacaag cagactcgac ggcgccggaa aaaagcacca gccgaggttc
     7921 ctgatactct tgcccgttgc ggcggaagat ttctccccag gtgcgttcgg tgctggcaag
     7981 cacactggaa acaaactgac ccatttcatc ggtggcgcct tgttgcggaa cttgctggga
     8041 cggcgggccg ctatcgatgt ttccttgcaa taacacgctg ggatcaatgc cgaagaatag
     8101 gccgatcagg acgatagcga tcgtgccgat cccgcctcca gccaatcccc gggacattct
     8161 catgccgcgg cgatcttcaa cgtttgtgct ttggccttct tgatcgagtc gcatacaagc
     8221 tccttcgctg tgaatctcca ggaccgctac cgcggctgca gcagcagtct cctccagaga
     8281 ctaaagtgtt caaacagata acttctctac cacgcgatgc atagacttcc ctagccaata
     8341 accctcttat cgaaatcttc tcgaaccgtc tagagcatct gtgtctcaca gtcggatgga
     8401 actcgctgcc gccgtcgatc agcaggttgc gatccgccgg tggacagatg ctatctattt
     8461 acttaagttg cttagaccgc atatatggct ttgcatcaaa ggcgctctca ggcggatcct
     8521 tttgcgcgcc gccgtgcgcc ttgacaattt catgcatacg tttaagcgag gttatgtctt
     8581 gcggctcgaa agaggccata tacgcgccgt cccactggct cgcgtatgca gtggcgtctt
     8641 tgttatccat gttggcggct ttctgaagta tctccgcaac tcgcgttttg tttttcgatc
     8701 cgtattcgac ggttttcctc atcgccacga taaacttcgc gaccgcgtcg gcattcttcg
     8761 tcaggtaatc gttgtacacg atggtgacgc caagaatcgg cgctgcgctg ctcttggtga
     8821 gctttttcca ttcgtcgacg atgctcgcaa gccggcgcaa cttggtttcc tccatctgtg
     8881 ctatcgtaac cgagcgaatg atcgccgcat cgatttccct ctgcgtcaag aactgcgcca
     8941 gccgcgcttc gttccccggc gcgacgctgt attccttcgg tttgatgccg tagttctgat
     9001 cgagaatcgc ggtgctaatg gcgtgggtcg cgctgccggg cggcgacatg ccaattttct
     9061 ttcccttgag atcggcagcc gttttaatcg gcgcaccatc gagcacaacc acctgtgcct
     9121 cggcaatctg cgttgccaaa acgatcttca ccggcacgcc gtcagcggca atgccgatgg
     9181 agttagtcga tggcgcggcg aaggcaagat cgatcgcatt ggaaacgatc gcgcgagtgg
     9241 gtccgttgac ttcggcaaac ttcacccatt cgacctcgag gccctctttc tcggccaatt
     9301 tcatttgctc caaaactgcg ccgaatccca ggctaaaacc gctggtccaa taaccaactt
     9361 taattctttg agcgagcagc tcgcgtggca tgaaacccca ggccgcgagt acaagcgcga
     9421 aagcagcagc gctgctgatc agtttttcga ttctcacatt tacctccgtt tttttggggc
     9481 gtgcgttcaa ctgctcccgt ccggtcgcta ttcaattgcg gagctctgac gttgaacgtt
     9541 ggaataggcc ctacggccgg ttgaacagtt gaacaattcc ttttaacaga gaaaaacctt
     9601 tcgctaatcg tttttagata agtcctcgca atttctgtat atagtttgag acttccgcct
     9661 gggtcgtgtc gcgcggacgc gctagggcta tcggaacgat ctcccggatt gtgccgggcc
     9721 gcggcgacag caccgctact tcatcggcga gaatcaccga ttcctcgacg tcgtgcgtta
     9781 cgaacactac cgtcatgcgt ttgatctcat gtatccgcag cagttcctcg tgcatcagcc
     9841 cgcgggtttg cgcgtcgagc cgagaaaacg gttcatccat taaaagaatg cgtggctcgg
     9901 tgaccaggct acgcgccaga gcgacacgac tgcgcatgcc gccggagagc tgatagggaa
     9961 acgatgcttc aaaaccattc aaatcaacca gctccaaggc ttggccggcg cgccgaatcc
    10021 tttcactttt tggcatcgct cccgcttcca aagcgaattc aacattgcgc atcgcgttgc
    10081 gccatggcaa caatcgatcg tcttgaaaca tataactgat cgtgcgccag tcgttgaatt
    10141 ccgcgctggg cgcgccgcag atgcacacct ggccatggtc cggcttaaca atgccggaga
    10201 tgatgttaag caaggtgctc ttgcctgagc ccgatgcgcc gagcaatccg acgatagcgt
    10261 gatcggccac ggtaagattg acatccttca gcacttcaag ccggccatcg ccggcagcaa
    10321 aactcttgct gatgccgctc aggcggatgg cggctgcggg ctgccccggt tcaatgcacg
    10381 cttgctgcgc taaactcgga ttatccattg gtttcaataa gcgctaatcg tcatcgtttc
    10441 gagagtaaaa tgcgtctgct gaaaagacaa tttccgtttt cattgccctt ccgcttcttt
    10501 cttccagcga aacaggtgat tctccagcgg ctttagcaag cccatttcca aggtagccat
    10561 catagcaacc agagttagag tccaggcaaa tacctggtcg gtctgtatca gatccgctgc
    10621 ctggcgcaga cgatagccga ctccgctcga ggcgcctatg gtctcggcga ccagggagac
    10681 gcgccagccg aaaccatacg ccaggcgcgc gccggagaaa aagtaaggaa gtatggtcgg
    10741 cagatagatt ttggtcatca ctttccattt cggcatgcgc agcacctgag ccaattcgat
    10801 gaactccgcg ttgaccgtgc gtgtaccctg ccaaacgttg gtaatgatca atggcatggc
    10861 agtcatgaac acgacaaaaa tagtggtggc gttggagatg ccgaaccaga tgatggcgaa
    10921 gatggcccag attgcagagg acaccgtgtt cagcaccggg atgaccggct cgaagaattc
    10981 gcccgcgcgt ttgttcgctc ccaggaagat gccaaacggc aaacttacaa gcgccgcaat
    11041 gacaaacccc acagtgaccc gccagaaggt aatgccgaga ttactccaca gatcgccgtt
    11101 ggcggcaata aggctgagcg cctgccctac gcgcgccggc ccaggcaaaa tgtaatgcgg
    11161 caccgacaaa gagaacagcc accagatgcc gatgaacaac gcaacgagaa cgacacgctg
    11221 aagaagaagg tcaaagcgca gcttgcgggg gttggaacgc gtccacacgt tatctgcaac
    11281 tgcttcggtc gtctcatctt tcatctgttc tcttatagga gcgcgcccta tgattgtgcc
    11341 tatcacagtt tctcgccgcg ccaaatcttg ccacaaacta tacttcggga ttcagagcga
    11401 ggcgagcaac gtcaaagaga tgatgggaaa ttagagaagt atgggagacc ttcgattgtt
    11461 gcgcatgacg cgagaacggt gcgtcacggg aagctcaagc caacgaagtc gtctggtcag
    11521 tgacaatata tgggacagac gtgccgtctt gcgtcggctt ataagcaaag cgggcgtgcc
    11581 cggaataatg gcaaggagat atgtaacgat aacggttaga ccgtgcgctt ggcttgcgcc
    11641 gatgccgcga tttcggctaa cagcgcgatc atcttgggat gagccggtca ggctgccggc
    11701 agaagttctt cccccgatac aaactcatcg aagggcttgg ccttgtatgg caccgacccg
    11761 agaccgcagc gctatata
//
LOCUS       DASUVS010000153        14043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1590306, whole genome shotgun sequence.
ACCESSION   DASUVS010000153 DASUVS010000000
VERSION     DASUVS010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14043
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590306"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VF182_08610"
     CDS             <1..807
                     /locus_tag="VF182_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX6437180.1"
                     /translation="GVISRILPEEDMPYLEDGTPVDVVLNPLGVPSRMNVGQILEAHL
                     GWAARSLGHQIDQLMLEHRQEVDGLRKRLKEYLNTKEANELIDGVKDADVFKLAEKYR
                     DGVRLASPVFDGAAEEEIFNLLKKADLPVSGQVTLYDGRSGEPFDGKVTVGVMYIMKL
                     HHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRLGEMEVWALEAYGAAYTLQEMLTV
                     KSDDVVGRTRMYEAIVKGDNLLEPGLPESFNVMVKELQSLGLDVDLVEEK"
     gene            852..4979
                     /gene="rpoC"
                     /locus_tag="VF182_08615"
     CDS             852..4979
                     /gene="rpoC"
                     /locus_tag="VF182_08615"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX6437181.1"
                     /translation="MEDLFNLFERPKNPLSFNAIRVSVASPEKVRSWSHGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPTKDYECNCGKYKRMRHRGVVCEKCGVEVIQSKVRRERM
                     GHIELATPVVHIWFLKSLPSRIGALLDMSLKDIEKVLYFESYIVIDPGETPLQYKELL
                     SEARYRKAMEEFGGRFTAEMGAEAVRKILKAVDVVKLSEDLRQEMRDANSEVRRKKLA
                     KRLKVLNAFRTSGNRPEWMVMEVVPVIPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLMELSAPDMIIRNEKRMLQEAVDALFDNGRRGRAITGQNRRPLKSLSDMLKGK
                     TGRFRQNLLGKRVDYSGRSVIVVGPELRLHQCGLPKKMALELFKPFIYNKLEERGFVT
                     TIKSAKKMVEKERPEVWDILDEVIREHPVMLNRAPTLHRLGIQAFEPILIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLSVEAQVEARALMMSTNNILSPANGKPIIVPTQDIVL
                     GLYYMTRDRLQAKGAGRIFSSPTEVRIAYDQGEVEIQAPITVRMNGERLETTVGRILV
                     YEVVPPEISFKEVNRVLKKKELANLIDISYRLAGNKATVILADRLKDIGYRHATKAGI
                     SIAIKDMVIPPNKEVLLKSAHEDVREIEEQYKNGLITDGERYNKVVDIWAEVTDRIAD
                     EMLRDLGSEKVTDETGRETSIPSFNPIFMMADSGARGSAQQIRQLAGMRGLMAKPSGE
                     IIETPITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDSVISD
                     PDCGTMDGIEITPLMEGGEIIEPLGDRVLGRVALEDVRDPFSGDVIVKANQEINEDLV
                     QRIEDAGLERIKIRSVLTCQSKRGVCVMCYGRDLGRGHMVNHGEAIGVIAAQSIGEPG
                     TQLTMRTFHIGGTASRRAEQTTLESRNDGVIKFINVSTVTNKEKDLVVMNRNGELAIV
                     DYPEGEKGRERERERYPVVYGAKLKKKDGEKIKAGELIAEWDPYTIPILTEMSGVVKF
                     GDVVEAITMEEKVDERTGLSTKVIIDSKDVDKRPRISIKDDGGQTARMSATSEARYLL
                     PVGAHINVQEGQTVYAGDAIAKIPRETTKTKDITGGLPRVAELFEARKPKEFAVISEI
                     DGTVSFGKDTKGKRKVVVTPEVGEPREYLIPKGKHISVHETDRIRAGEPLMDGSSNPH
                     DILNILGEKALAKYLVDEVQEIYRLQGVRINDKHIEVIVRQMLRRVRIKEVGDTEFLV
                     GDQVEKWRFDEENQRAIAAGGRPAVAEPLLLGITKASLSTESFISAASFQETTKVLTE
                     AAIHGKIDLLCGLKENVIMGRLIPAGTGVAKYSKAEIEIDAPEGFEEDMSQAAGA"
     gene            5081..5452
                     /gene="rpsL"
                     /locus_tag="VF182_08620"
     CDS             5081..5452
                     /gene="rpsL"
                     /locus_tag="VF182_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX6437182.1"
                     /translation="MPTISQLVRESREKQQRKNTAPALQGCPQKRGVCTRVYTSTPKK
                     PNSALRKVARVRLTNGIEVTSYIPGVGHNLQEHSVVLIRGGRVKDLPGVRYHIIRGTL
                     DSIGVQERRQGRSKYGAKKPK"
     gene            5454..5924
                     /gene="rpsG"
                     /locus_tag="VF182_08625"
     CDS             5454..5924
                     /gene="rpsG"
                     /locus_tag="VF182_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX6437183.1"
                     /translation="MPRKGEVRKREVLPDPKFGEKLVTKFVNTIMNQGKKSVAESILY
                     RSLDIVAAKTKEDSIGIFKKAIENTRPSVEVRSRRVGGATYQVPVEVRPQRRISLSMR
                     WLIQAARDRSEKSMEEKLAAELLDAANNRGTAIKKKEDTHRMAEANRAFAHYRW"
     gene            5990..8086
                     /gene="fusA"
                     /locus_tag="VF182_08630"
     CDS             5990..8086
                     /gene="fusA"
                     /locus_tag="VF182_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX6437184.1"
                     /translation="MPRTVPLDRTRNIGIMAHIDAGKTTTTERILYYTGINYKIGEVH
                     EGTATMDWMVQEQERGITITSAATTCLWRDHRVNIIDTPGHVDFTIEVERSLRVLDGA
                     VGVFCSVGGVEPQTETVWRQADKYQVPRIAFVNKMDRLGADFFRVVQMIKDRLGARPV
                     PLQLPIGAEDKFIGVIDLVRMKAIVWEDESLGAKFRDEAIPEEMASAAAEYREKLIEA
                     AADSDETIMEKYLEGHEISEAELTKAIRSATLAIKIIPVLCGSAFRNKGVQPLLDAVV
                     AYLPSPLDIPPVKGIDPDSQSADERPAKDDAPFSALAFKIMTDPFVGTLSFFRVYSGS
                     LTAGSSVYNSTKGKRERVGRLLKMHANKREEIKEVYAGDIAAGVGLRTATTGDTLCDE
                     NDPIILESIEFPEPVISIAIEPKSKADQEKLGLSLQKLAAEDPSFRVRTDEETGQTII
                     SGMGELHLEIIVDRLLREFNVGANVGKPQVAYKETVRKAVEQQGKFIRQTGGRGQYGD
                     VWIKLEPQEPGAGFEFVDAIKGGSIPREYIPAVEKGVKEATENGALAGYPMVDVKVTL
                     FDGSYHDVDSSEIAFKIAGSMAFKEAARKASPVLLEPIMSVEVVVPEDFMGDVIGDLN
                     SRRGKVLGMDTRPAAQAIDARVPLAQMFGYATDLRSMTQGRATYTMQFSHYEPVPANV
                     AEGIIAKLDGKSDDKK"
     gene            8083..8391
                     /gene="rpsJ"
                     /locus_tag="VF182_08635"
     CDS             8083..8391
                     /gene="rpsJ"
                     /locus_tag="VF182_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX6437185.1"
                     /translation="MNEKIRIRLKAYDHRVLDQSVGEIVETVKRTGGRVAGPIPLPTR
                     IERFTVNRSPHVDKKSRDQFEIRTHKRLLDILEPTQQTIDALGKLDIAAGVDVEIKLQ
                     "
     gene            8432..9064
                     /gene="rplC"
                     /locus_tag="VF182_08640"
     CDS             8432..9064
                     /gene="rplC"
                     /locus_tag="VF182_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013614173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX6437186.1"
                     /translation="MTGLIGKKLGMTQFYSANGNVVAVTVIEAGPCVVVQKKEAAVDG
                     YNALQVGFGSKKGQRINKPRQGHMAKAGKGAFSTLREFRSEDVTGYQVGQEIKINDLF
                     KIGDLIDVSGVSKGHGFSGVIKRWSFAGFPGSHGTHEYFRHGGSIGNRSYPGRVRKGK
                     KMAGHWGNENISIQNLEVVDVRAEENLLLVKGAVPGAKQGIVILRRAVKG"
     gene            9071..9667
                     /gene="rplD"
                     /locus_tag="VF182_08645"
     CDS             9071..9667
                     /gene="rplD"
                     /locus_tag="VF182_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX6437187.1"
                     /translation="MEVHVAEAKLDERIFGVNARPHLLHQVVVMQLRNRRAGTAATKS
                     KGFVRGGGKKPWRQKGTGRARSGSIRSPIWVGGGTIFGPQPRDYSFRMPRKARREALL
                     SALSLKNQDGKLIVLDKLELEEAKTRLMANALAELKVQSALLVVSQADAKVERAARNL
                     PKIKVLRVEGLNVYDLLRYEHLILMEGALKLLEERLAS"
     gene            9664..9954
                     /locus_tag="VF182_08650"
     CDS             9664..9954
                     /locus_tag="VF182_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX6437188.1"
                     /translation="MRGPQEIIKAPVISEKGSLLAETMHQVLFKVRPDANKVEVKKAV
                     ESLFKVKVVKVRMARYLGKVRRVGRSTGRRPEWKKAYVTLREGDKIDFFGAA"
     gene            9966..10793
                     /gene="rplB"
                     /locus_tag="VF182_08655"
     CDS             9966..10793
                     /gene="rplB"
                     /locus_tag="VF182_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX6437189.1"
                     /translation="MAIRTYRPTSAGMRFRTGLTFDEIAKKRPEKALRTPLGHSGGRN
                     NNGRITADHRGGGHKRLYRVIDFKRNKKGVPAKVEAIEYDPNRSANIALLCYVDGERR
                     YILAPERLKVGDSVIAGDDNIDIQPGNSLPLKFVPVGTVVHNVELKIGKGGQLARSAG
                     SGIQLMAKEGSYALLKLPSGELRNVLAECRATIGQVGNADQENVSWGKAGRMRWLGFR
                     STTRGIAKNPVDHPHGGGEGRSKGNHPMTPWGKPTKGYKTRKNQSTDKYIVKGRKAK"
     gene            10795..11103
                     /gene="rpsS"
                     /locus_tag="VF182_08660"
     CDS             10795..11103
                     /gene="rpsS"
                     /locus_tag="VF182_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX6437190.1"
                     /translation="MPRSIKKGPFVDEHLTRKISAMNESRQKQVIRTWSRRSTVTPEM
                     VGHTIGVHNGRKFIPVFISENMVGHKLGEFSPTRTFFAHSGDKKGETGQSGPRPAGGR
                     "
     gene            11163..11507
                     /gene="rplV"
                     /locus_tag="VF182_08665"
     CDS             11163..11507
                     /gene="rplV"
                     /locus_tag="VF182_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX6437191.1"
                     /translation="MEARAITKFIRLSPRKVRLVVDQIRGKGVEEALNILKFTPKRSA
                     LIVSKTLRTAVANAESTQSVDVDQLYVKQAKVDEAGMWKRFTPRAQGRATRIRKRLSH
                     VTIVLDEVSERE"
     gene            11509..12171
                     /gene="rpsC"
                     /locus_tag="VF182_08670"
     CDS             11509..12171
                     /gene="rpsC"
                     /locus_tag="VF182_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX6437192.1"
                     /translation="MGQKTHPKMFRLGVIESWDSKWYSRHDYAKLLHEDFRVKKFLKS
                     RLYHAGISKIEIERAANKAKINIHTARPGIVIGKKGAEIEKLKEEIGKLTNRETYINI
                     HEVRRPDLDGQLVAENVALQLERRVAFRRAMKESVSRAMRMGAQGVKIQCAGRLGGSE
                     IARTEWYREGRVPLHTLRADVNYGFAEARTTYGIIGVKAWIFRGEVLTEEEEQQKAML
                     GG"
     gene            12209..12634
                     /gene="rplP"
                     /locus_tag="VF182_08675"
     CDS             12209..12634
                     /gene="rplP"
                     /locus_tag="VF182_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX6437193.1"
                     /translation="MLEPKKVKYRKIQKGRRRGTAYRGSTLAFGDFGLKAIDRGWMSA
                     REIEAARVALTRYLKRGGKVWIRIFPDKPLTKKPAETRMGKGKGSPEMWVAVIQPGRI
                     LFEMEGVEANLAREAFRLAAHKLSIPTIVVERKGRVHGN"
     gene            12624..12812
                     /gene="rpmC"
                     /locus_tag="VF182_08680"
     CDS             12624..12812
                     /gene="rpmC"
                     /locus_tag="VF182_08680"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX6437194.1"
                     /translation="METKELRDMNSADLARKLSELREEIGHLKLKQATSRLENPMKLR
                     QTKRELARVETILKEKAV"
     gene            12830..13123
                     /gene="rpsQ"
                     /locus_tag="VF182_08685"
     CDS             12830..13123
                     /gene="rpsQ"
                     /locus_tag="VF182_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX6437195.1"
                     /translation="MDTRNLRKERTGTVISDRMEKSIVVSLERTVMHPKYRKYLKRRS
                     RVKAHDETNQCHVGDRVRIVECRPLSRDKRWRVSEILQRTRTTDGEQPVGGDV"
     gene            13158..13526
                     /gene="rplN"
                     /locus_tag="VF182_08690"
     CDS             13158..13526
                     /gene="rplN"
                     /locus_tag="VF182_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX6437196.1"
                     /translation="MIQTRTVLDVADNSGARKVQCIKVLGGSKRKYASIGDIIVVSVK
                     EAIPNAKVKKGEVMKAVVVRTAKEIGRSDGTYIRFDNNSAVLIDNQKEPIGTRIFGPV
                     ARELRAKKFMKIISLAPEVL"
     gene            13548..13859
                     /gene="rplX"
                     /locus_tag="VF182_08695"
     CDS             13548..13859
                     /gene="rplX"
                     /locus_tag="VF182_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX6437197.1"
                     /translation="MQIRKNDSVMVISGKERGKTGKVLRVNPKEDAVIIERINVVKRH
                     TKPRGPQQAGGIIEKEASIPGSNIMIMCDKCNAPVRIGRKLLTDGKKVRVCRRCGEAL
                     D"
     gene            13871..>14043
                     /gene="rplE"
                     /locus_tag="VF182_08700"
     CDS             13871..>14043
                     /gene="rplE"
                     /locus_tag="VF182_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX6437198.1"
                     /translation="MVRLKQKYAEQVVSAMKSEFGYKNSMQVPKLEKITINVGLGEAT
                     QNIKALDSTVAEIT"
BASE COUNT         3613 a         3235 c         4149 g         3046 t
ORIGIN      
        1 ggtgttatct cgaggatcct gcccgaagaa gacatgcctt atctggaaga tggcactccg
       61 gttgacgtgg tgctcaatcc tctgggtgtg ccttcacgta tgaacgtggg ccagattctc
      121 gaagcgcatc ttggctgggc agctcgtagt ctcgggcatc aaattgatca attgatgctg
      181 gaacaccggc aggaggtaga cggactgcgc aagcgtctga aggaatatct taataccaag
      241 gaagccaacg agctgatcga tggcgtcaag gacgcggacg tattcaagct ggcggagaag
      301 tatcgtgacg gcgtgcgctt ggcgtcgccg gtttttgacg gcgcggcgga agaggaaatc
      361 tttaatctac tgaaaaaggc cgatctcccg gtgagcggcc aagtgactct gtacgatggc
      421 cgtagcggcg agcccttcga tggcaaagta acggtcgggg tcatgtatat catgaaactg
      481 catcacctgg ttgatgacaa aatacatgct cgctccaccg gtccgtattc tctcgttacc
      541 cagcaaccat tgggtggaaa ggctcaattc ggcggccagc ggctcggaga aatggaggtc
      601 tgggccctag aggcttatgg agccgcgtat actcttcaag agatgctgac ggttaaatcc
      661 gatgatgttg tcgggcgaac gcgaatgtac gaggcgattg ttaaagggga caatctcctc
      721 gaaccagggt tgccggaatc ttttaacgtc atggttaagg agctccagag tttggggctc
      781 gatgttgatc tagtcgagga aaaatagcta agcctgtaca cccacagcgc tgatgctgga
      841 ggtcgatctt tatggaagat ctttttaatt tattcgagcg accaaaaaat ccgctaagtt
      901 ttaatgcgat ccgagtctct gtcgcatccc cggaaaaggt ccgatcttgg tcacacgggg
      961 aagtaaagaa gccggaaacg attaactaca gaacgttcaa gcccgaacgg gatggtttat
     1021 tttgcgccaa gatttttggc ccgaccaagg actacgaatg taactgcggc aagtataaac
     1081 gcatgcggca ccgaggcgtt gtctgtgaaa aatgcggcgt cgaggtgatt cagtcaaagg
     1141 tgcgccgcga gcggatgggc catattgagc tggccacgcc ggtagtccat atctggtttc
     1201 ttaagagcct gccgagccgg attggcgcct tactcgatat gtccctaaag gacatcgaga
     1261 aggttctcta tttcgaatcc tatatcgtta tcgatccagg cgagactcct ttgcaataca
     1321 aggagctctt gagcgaggcc cgttatcgca aagcaatgga agagttcggc ggcagattta
     1381 ctgcggagat gggagcagag gcagttcgga agatcctcaa agcggtcgat gtcgtaaaac
     1441 tttcagaaga tctgcgccaa gagatgcgcg atgcaaattc cgaggttcgg cgcaaaaaat
     1501 tggcaaaacg gttaaaggtc ctcaacgctt ttaggacgtc tgggaatcga cccgaatgga
     1561 tggtcatgga agttgttccg gtcattccgc cggatttacg gccattggtg ccacttgacg
     1621 gtggtcgctt cgcgacctcg gatttaaacg atctctaccg gcgggtgatc aaccggaata
     1681 atcggctcaa gcgcctaatg gagttaagcg ctccggatat gattatccgg aatgagaaac
     1741 gcatgctcca ggaggcggtt gacgctttat tcgataatgg ccgccgcggg cgagctatca
     1801 cggggcaaaa tcgacggccg cttaagtctt tgagcgatat gctcaaaggt aaaaccggta
     1861 gattccgcca gaatctgctt ggcaagcggg tggattattc cggacgttcg gtgatcgttg
     1921 ttggaccgga attgcggctg caccaatgtg ggctgccaaa gaagatggct ttggagttgt
     1981 tcaagccatt tatctataac aagctcgaag agcgcggctt cgtcaccact atcaagagtg
     2041 cgaagaaaat ggtcgagaaa gaacggcccg aggtgtggga catcctcgat gaagtaattc
     2101 gcgagcatcc ggtgatgtta aaccgcgctc cgacattgca ccgccttggg atacaagctt
     2161 ttgagccgat tctgattgaa gggaaggcga ttcagcttca tccattggta tgcgcggcgt
     2221 ataacgcaga tttcgacggt gatcagatgg cggtgcatgt tccattgtcg gtcgaagctc
     2281 aagttgaagc tcgcgccctg atgatgtcca ccaacaacat cctgtcaccg gccaacggga
     2341 agccgatcat cgtgccgacc caggatatcg tgttggggct ttactatatg acccgcgacc
     2401 gcttgcaggc taaaggagcc ggccgcatct ttagcagccc caccgaagtt cgaatcgcct
     2461 atgatcaggg tgaagtggag attcaagcgc cgattaccgt tcgaatgaat ggcgagcgcc
     2521 tcgaaaccac cgtcggccgg attctagtat atgaggttgt gccgccggag atttcgttta
     2581 aggaagtcaa tcgagttctg aagaaaaaag agctggcaaa cctgatcgac atcagctacc
     2641 gcttggccgg caacaaggcc acggtaatcc tcgcagatcg gttgaaggat atcggctatc
     2701 ggcacgccac taaggcgggc atctctatcg ccatcaagga tatggtgatc ccgcccaata
     2761 aagaggtcct gcttaagagc gcgcacgaag atgttcgcga aatcgaggag caatacaaaa
     2821 acggactcat taccgacggg gaacgttaca acaaagtggt tgatatctgg gctgaggtta
     2881 ccgaccgaat cgccgacgaa atgctacgcg atcttggctc cgagaaagtt accgatgaaa
     2941 ccgggcgtga aacttctatc ccgagcttca atcctatctt catgatggcc gattccggtg
     3001 ctcgtgggag cgcgcagcaa attcgtcaat tggcaggcat gcgcgggttg atggccaagc
     3061 catccggaga aattatcgag acgccgatta ccgctaattt tcgcgagggc ttgacggttt
     3121 tgcagtactt catctcgact cacggcgccc gtaaggggtt ggctgatacg gcattgaaga
     3181 ccgctaactc cggttatctg actcggcggc ttgtcgatgt agctcaggat tcggttattt
     3241 ccgatcctga ttgcggcacc atggacggca tagagatcac gccgttgatg gaaggcggtg
     3301 agattatcga accgctcggc gatcgcgtgt tggggcgagt tgcgctcgag gatgtccgcg
     3361 atcctttctc gggcgacgtt atcgttaaag ccaaccagga aatcaatgaa gacttggtgc
     3421 agcgcattga agacgccggt ttggagcgga taaagattcg ctcggttttg acctgccaat
     3481 ccaagagagg cgtgtgcgtc atgtgttatg gccgcgatct aggccgagga catatggtca
     3541 atcatggcga agcaattggg gttattgcag cccaatcgat tggtgaaccg ggaacacagc
     3601 tcaccatgcg tacgtttcac ataggtggta ctgcgagcag gagagcagag caaacgactc
     3661 tcgagtcgcg caacgatggg gttatcaagt tcatcaacgt cagcaccgtc accaacaagg
     3721 agaaggatct agtggtgatg aaccgcaacg gcgaacttgc gattgtggat tatccggaag
     3781 gtgagaaggg tcgggaacgg gagcgagagc gttatcccgt ggtttatggc gccaagctga
     3841 aaaagaagga tggagaaaag atcaaagccg gtgagttgat tgccgaatgg gatccataca
     3901 cgattccaat tttgacggaa atgagcggcg tcgtgaaatt tggcgacgtc gtcgaagcga
     3961 tcaccatgga agagaaggtg gatgagcgca caggtctgtc cacgaaggta atcattgatt
     4021 ccaaggacgt cgacaagcgg ccgagaatct ccatcaaaga cgatggtgga cagaccgcgc
     4081 gtatgtcagc aacatcggaa gctcgctatt tgctaccggt gggcgcgcac atcaacgtgc
     4141 aagagggtca gacggtttat gccggggatg cgattgcgaa aatcccgcgc gaaacaacta
     4201 agacaaagga tattaccggc ggcttgcccc gggtagctga attgttcgaa gcgcgcaagc
     4261 cgaaagagtt cgcagtcata agtgaaatcg acggaaccgt ttcctttggt aaagacacca
     4321 aaggaaagcg caaagtggtc gtcactcccg aagttggcga accgcgcgag tatctgatcc
     4381 ccaagggtaa acacatcagc gtgcacgaaa ccgatcgtat acgcgctggc gagcctctca
     4441 tggatggttc ttcaaatccc catgacattc tcaatatttt gggtgaaaaa gcgctcgcca
     4501 agtaccttgt cgacgaggtt caggagattt atcgcctgca aggggtgcgc attaacgata
     4561 agcacatcga ggtcatcgtt cgacagatgt tacgccgtgt gcgcatcaaa gaggtcggcg
     4621 ataccgaatt cctcgtcggc gatcaagtgg agaaatggcg ctttgacgag gagaatcaac
     4681 gcgccatcgc agcaggtggc aggccggctg tggctgagcc actgcttctc gggatcacca
     4741 aggcctcgct atcgaccgaa agctttatct cggcagcttc gtttcaagaa acgacgaaag
     4801 tgctcactga agcggcgatt cacggcaaga ttgatttact ttgcggtctt aaggagaacg
     4861 tgatcatggg ccgattgatc ccagccggga cgggggtcgc aaaatattcg aaagctgaaa
     4921 tcgaaatcga tgctccggaa ggatttgaag aggacatgtc gcaggccgcg ggagcgtagg
     4981 gttttgacat ggtgggcata atttgttaac taaagtggtt ccccaggaaa tttgtcgcct
     5041 tatccacata aagtttattt ttgggtgatt aaacagacgt atgcctacaa ttagtcaatt
     5101 ggttcgcgag agccgcgaaa agcagcaacg taagaacaca gcgccggcac tacagggctg
     5161 cccacaaaag cgcggggttt gtacacgtgt ctatacttcg accccaaaga agccgaattc
     5221 ggcgctacgt aaagtggctc gtgttcggct gaccaatggg attgaagtga cctcgtacat
     5281 ccccggggtt ggtcacaacc tgcaagaaca ttcggttgta ttgatccgtg gagggcgcgt
     5341 caaagatctt cctggagtcc gctatcacat cattcgtgga acactcgatt cgattggtgt
     5401 gcaagagagg cggcaaggtc gttccaaata cggagcgaag aaaccgaagt aatatgccac
     5461 gcaaggggga agtcagaaag cgagaggttc tgccggatcc aaaattcggt gagaagcttg
     5521 ttaccaagtt cgttaacacg atcatgaacc agggaaagaa gagcgtggcc gaatcgattc
     5581 tctaccggtc cttggacatt gttgctgcca aaaccaagga agattcgatt gggattttca
     5641 agaaagcgat tgagaatacc cggccatcgg tcgaagttcg atcgcgccgt gtcggcggtg
     5701 cgacctatca ggttccagtc gaagttagac cgcaacgccg tatctctttg agcatgcgct
     5761 ggctgattca agcagcgcgc gatcgcagcg agaagtccat ggaagaaaag ctcgccgctg
     5821 aattgttgga cgctgcgaat aatcgaggta ccgcaataaa gaaaaaagaa gacactcatc
     5881 gtatggcgga ggcgaaccgc gcctttgctc attaccgatg gtagattgag aaaaaatatt
     5941 agtaaaagga ggcggttgat ccgcctcctt tttttcccgg tgtagagcaa tgcctagaac
     6001 tgttccctta gatagaaccc gcaatatcgg catcatggcc catattgatg cgggaaaaac
     6061 gacgaccacc gaaaggatcc tttactatac agggatcaac tacaagatcg gtgaggttca
     6121 cgagggaact gccacgatgg actggatggt ccaggagcag gagcgcggca ttacgatcac
     6181 ctcagctgca accacctgct tatggcggga ccaccgggtc aatatcatcg atacgccggg
     6241 acacgttgac tttaccatcg aggtggaacg ttcgctccgg gttctagacg gtgcggtggg
     6301 ggttttctgc tctgtgggtg gagtcgagcc gcagaccgag actgtttggc ggcaggccga
     6361 taagtatcaa gtcccgcgca ttgcattcgt caataagatg gatcggctag gtgcggactt
     6421 cttcagggtc gttcagatga taaaggatcg tctcggcgcg cgcccggttc cgttgcagtt
     6481 gccaatcggc gccgaagata aattcatcgg tgtcattgat ttggtgcgca tgaaagcaat
     6541 cgtttgggag gatgagagtc ttggcgcaaa gtttcgtgat gaagccatac cggaagagat
     6601 ggcatcggcg gcagcggagt accgtgaaaa gttaatcgaa gcggctgcgg attcggacga
     6661 gacgatcatg gagaagtact tggaggggca tgaaatcagc gaggctgagc tgaccaaggc
     6721 catcaggagt gctactttgg cgatcaagat cattccggtg ctgtgcggtt cggcgttccg
     6781 caacaaaggt gttcagccct tgctcgatgc cgtggttgca tatttgccat cgcccttgga
     6841 tattccgccg gttaaaggca tcgatcctga ttcccagtcg gccgacgaac gccccgccaa
     6901 ggacgatgct ccgttttcgg ctcttgcttt caaaatcatg acggacccgt tcgttggaac
     6961 gttgagcttt ttcagggttt actccgggtc gttaactgcg ggctcgagtg tttacaattc
     7021 aaccaaaggc aagcgcgaga gggttggtcg tctcttgaag atgcacgcga acaagcgtga
     7081 agagataaaa gaagtgtacg ccggtgatat tgccgctgga gtgggtctgc gcactgccac
     7141 aacgggtgat actttgtgcg atgagaacga tccgatcatt ctggagtcga tcgaatttcc
     7201 tgagccggta atttccattg ccatcgaacc gaaaagcaag gctgatcagg aaaagctcgg
     7261 attgtcactg caaaagctcg ccgcggagga tccgtccttt cgggtgcgga ccgatgaaga
     7321 gaccggccag acgattattt caggcatggg tgagcttcat cttgagatca ttgttgatcg
     7381 acttctaaga gaatttaacg tcggagccaa tgtcggtaag ccgcaggtcg cctataagga
     7441 aaccgtccgt aaggcggtcg agcagcaggg caagttcatt cgccaaacgg gtggtcgcgg
     7501 tcagtatggt gacgtctgga tcaagttaga gccacaagaa cccggagcgg gcttcgagtt
     7561 tgtcgatgcg atcaagggtg gttcgatccc gcgcgagtat attccagccg tagaaaaagg
     7621 cgttaaagag gcaactgaaa atggcgcctt ggcgggctat cccatggttg atgtgaaggt
     7681 cacattgttc gacggcagtt atcatgacgt cgactcgtcg gaaatcgctt ttaagattgc
     7741 cggctccatg gctttcaaag aggccgcccg caaggccagt ccggtcctgc tcgagcccat
     7801 tatgtcggtt gaggtcgtag tgcctgaaga ttttatgggt gacgtcatcg gcgatcttaa
     7861 ctcgcgccgt ggtaaagtct tgggaatgga tacgcggccg gctgctcaag caatcgatgc
     7921 gcgggtgcca ctggcccaaa tgtttggtta tgctaccgac ctgcgctcga tgacccaagg
     7981 gcgagcaacc tacacgatgc aattctcaca ctatgagccg gtgccggcga acgttgctga
     8041 gggcatcatt gccaagttag atggcaagtc ggatgacaaa aaatgaacga gaaaattagg
     8101 attaggctga aggcctacga tcatcgggtc ttggatcagt cggtggggga gatcgtcgag
     8161 acagtgaaac gcactggcgg cagagtggct ggcccgattc cattgccgac acggatcgag
     8221 cggttcacgg tcaatcgatc gccgcacgtc gacaaaaaat cccgagatca attcgagatt
     8281 cgaacccaca agagattgct tgatattttg gagccgactc aacagaccat tgacgcactg
     8341 ggaaagctcg atatcgctgc cggtgtcgat gttgaaatta agcttcaata agcaaccaag
     8401 cttattagga gctttcgctc gagagaatca tatgactggc cttattggga aaaaacttgg
     8461 catgacgcaa ttttattctg cgaatgggaa tgtggtcgcg gtcacggtca tcgaggccgg
     8521 gccttgtgtg gtcgtgcaga agaaagaagc ggcggtggac ggttacaacg ccctgcaggt
     8581 aggcttcggc agtaagaagg gccagcggat caacaaacct cgtcaggggc atatggccaa
     8641 ggcaggcaaa ggggcatttt cgaccttgag agaatttcga tccgaagatg tgacaggata
     8701 tcaggttggg caagagatca aaatcaatga tctgttcaaa atcggggacc tgattgatgt
     8761 ctccggggtt tcaaagggac atggattctc gggagtcatc aagcgatggt cctttgccgg
     8821 gtttccagga tctcatggta ctcacgaata ttttcgccac ggtggatcga tcggtaaccg
     8881 gtcgtatcca ggacgcgtgc gcaaaggcaa gaaaatggct ggtcattggg gcaacgagaa
     8941 tatctccatc caaaatttgg aagttgtaga cgttcgcgcc gaagagaatc tgctcctcgt
     9001 caagggagct gttccagggg ccaagcaagg catcgttatt ctccggcgag cggtaaaggg
     9061 ataagttgcg atggaagttc atgtggccga ggcgaaactc gatgagcgta ttttcggcgt
     9121 caatgcgcgc ccgcacctgt tgcatcaggt cgtcgtcatg cagctgagga atcgacgcgc
     9181 gggcacggct gcaaccaagt ccaaaggttt tgtccgcggc ggcggcaaga agccctggcg
     9241 gcaaaagggc actggtcgcg cccgctccgg cagtattcga tcaccgattt gggtcggagg
     9301 agggacgatc ttcggtccgc agccgcgcga ttactctttc cggatgccgc gtaaagctcg
     9361 gcgtgaagct ttgttgtcgg cgttaagcct gaagaatcag gatggaaagc tcattgttct
     9421 ggataagctg gagcttgagg aagctaagac caggctaatg gcgaatgctc ttgcggaact
     9481 gaaggtgcaa agcgccctgc ttgtggtttc acaggctgat gcgaaagtcg aacgggctgc
     9541 tcgtaatttg ccaaagatta aagtgctaag agtggaaggt ctgaacgtct acgatcttct
     9601 ccgttacgag cacctaattc tcatggaagg cgcattgaag ttgttggaag agagattggc
     9661 ttcatgagag gaccacagga aatcatcaag gcgccggtca tctctgaaaa aggctcgctc
     9721 cttgccgaaa cgatgcatca ggtcttgttc aaggtgcgcc cggatgccaa caaagttgaa
     9781 gttaaaaagg ctgtcgagtc tcttttcaag gttaaggtag ttaaagtgcg catggcccgt
     9841 tatttgggga aggtccggcg ggtcggcaga agtaccggcc ggcgaccgga atggaagaag
     9901 gcatacgtaa cgctacgcga aggcgataag atcgactttt tcggcgcggc gtagagagca
     9961 taaccatggc gattagaaca tatcgaccga catcggccgg gatgcgtttt cgaaccggct
    10021 tgacctttga tgaaatagca aagaagcgac ctgagaaggc attgagaacg cctttgggcc
    10081 atagcggtgg gcgtaacaat aatggacgca ttaccgctga tcatcgaggc ggcgggcaca
    10141 agcggctcta tcgcgtaatc gatttcaaac gtaacaagaa gggtgttccg gcaaaggttg
    10201 aagccattga atacgatcca aatcgttcag ccaacatcgc gctgctttgc tatgtggatg
    10261 gcgagcggcg gtacattcta gctcccgaac gtcttaaagt aggcgattcc gtgattgccg
    10321 gggacgacaa tatagacatt cagccgggca attcgctgcc tctgaagttt gttccggtag
    10381 gtactgtggt tcacaacgtc gaacttaaga taggcaaagg cggtcagttg gcccgaagcg
    10441 ccggttcagg cattcagttg atggccaaag aaggttctta tgctttgctg aagctgcctt
    10501 caggtgagct ccgtaacgtt ctggctgagt gccgcgcaac catcggtcag gtcggcaatg
    10561 ccgaccagga gaatgtttcc tggggcaagg ccggacggat gcgctggctc ggctttagat
    10621 cgacaacccg cggcatcgcc aagaatccgg tcgatcaccc gcatggcggc ggcgaaggtc
    10681 gctccaaagg aaatcatccg atgacaccgt ggggaaagcc aaccaaaggt tacaaaactc
    10741 ggaaaaatca gtccacggac aagtacatcg tcaaggggag aaaggctaaa taggatgcca
    10801 cgttcgatca aaaaggggcc ctttgtcgat gagcatctga cgaggaagat ttcggccatg
    10861 aacgaatcgc gtcagaagca agttatccga acctggtcgc gacgatctac ggtcacgccg
    10921 gaaatggtcg gtcataccat cggcgtgcac aacggtcgaa agttcattcc ggtgtttatc
    10981 tcggaaaaca tggtcgggca taaacttggc gagttctctc ccactcgaac cttttttgcg
    11041 cactcgggag acaagaaggg tgagacgggg cagagtggac caagaccggc cggcggcaga
    11101 tagtggccga cgatctagcc gagaatacaa catgtgtgca atcgaaggtc attaactgag
    11161 ttatggaagc tcgcgcaatt acgaagttta tcaggttatc cccccgcaag gtccgattgg
    11221 tagtcgatca gattcgcggc aaaggggttg aagaggcgct gaatatttta aaattcacgc
    11281 cgaagcgctc cgccctgatt gtatccaaga ctttgcgcac cgccgtcgct aacgccgaaa
    11341 gcacgcagag cgttgatgtg gatcaactct acgtcaaaca ggccaaagtc gatgaggccg
    11401 gaatgtggaa gcgatttacg ccgcgagcgc agggaagagc cacacgaatt cgtaagcggt
    11461 tgagtcatgt caccattgtg ttggatgagg tgagtgagag ggaatagcat gggtcagaaa
    11521 acacatccaa aaatgtttcg cttaggtgtg attgagtctt gggactcaaa atggtactcg
    11581 cgccacgatt atgccaagct gcttcacgaa gattttcgtg tgaaaaagtt tttgaagagc
    11641 cggctgtatc atgccggtat ctcaaaaatt gaaatcgaga gggccgctaa taaggctaag
    11701 attaatattc atactgcgcg tcctggaatc gtgatcggca aaaagggcgc cgagatcgaa
    11761 aaattaaaag aagaaatcgg caagcttacc aatcgcgaaa cctatatcaa tatccatgaa
    11821 gtcagacggc ccgatcttga tgggcagctc gtggccgaaa acgtcgcttt gcagctggag
    11881 cgtcgtgtgg cttttcggcg cgccatgaag gaaagcgtat cgcgggcaat gcgcatggga
    11941 gcgcaaggcg taaagattca atgtgcaggc cgattgggcg gttctgaaat tgcccgaaca
    12001 gaatggtatc gcgagggacg agttccgctc cacactcttc gggcggatgt caactatggt
    12061 tttgccgagg cgcgcaccac ctacggcatt attggcgtaa aggcctggat attccgaggc
    12121 gaagtgctca ccgaagaaga agagcagcag aaggcgatgt tgggcgggtg aagccggtcc
    12181 ggcttaaaat gatctgagat agaaaaagat gctcgaaccg aagaaggtaa aataccgaaa
    12241 aatacaaaaa gggcgccggc gaggaaccgc atatcgaggt tctactctcg cgtttggaga
    12301 tttcggcctg aaagcgatcg accggggttg gatgagtgcg cgggaaatcg aagcggctcg
    12361 agtcgctcta acgcgttatc tcaagcgagg cggaaaagtc tggatcagga tctttcctga
    12421 caagccgtta accaagaaac cagcggaaac ccgaatggga aaaggcaagg gatcgccaga
    12481 gatgtgggtt gcggtgattc aaccaggaag aattctattt gaaatggaag gcgtcgaggc
    12541 caacctcgcg cgcgaagcgt ttcgattggc ggcgcacaag ttgtccattc caaccatcgt
    12601 tgttgaaaga aagggcaggg ttcatggaaa ctaaggaatt gcgcgatatg aattccgcag
    12661 atctcgcgcg taagttgagc gaacttcgtg aagagattgg ccatttgaag ctaaagcagg
    12721 cgaccagcag gttggagaac ccgatgaagt tgcggcagac caagcgtgag ttggctcggg
    12781 tggagaccat cttgaaggag aaggcggttt aaggcgggaa ggcatagtta tggatactcg
    12841 aaacctgaga aaagagcgca ctggcacggt tattagcgat cggatggaaa agagcatcgt
    12901 ggtttctctc gagcggaccg tcatgcaccc gaagtataga aagtatttga aacgccgaag
    12961 ccgagtaaaa gcgcacgatg agactaatca atgccacgta ggcgaccggg tgcgtatcgt
    13021 tgaatgccgc cctctcagcc gtgataaacg ttggcgtgtt agtgagatat tgcaacgaac
    13081 ccgaacaaca gacggtgagc agcccgtcgg aggcgatgtc tagcgtccgt tcaggtcaag
    13141 taggttgagg ttgattcatg attcagacga gaacggtgtt ggatgttgcg gataattcgg
    13201 gcgcacgaaa agtccagtgt atcaaggttc taggtggcag caagcggaag tatgcgtcga
    13261 tcggcgacat catcgttgtg tcagtgaaag aagcaattcc taatgccaag gtaaaaaagg
    13321 gcgaagtgat gaaagccgtc gtggtacgga ccgctaaaga gatcgggcgc tccgacggaa
    13381 cttatatacg cttcgataat aattcggcgg tgctcatcga taatcaaaaa gaaccgattg
    13441 gaacccgaat ttttggtcct gtagcgcgtg agctgcgagc caagaagttt atgaaaatta
    13501 tttcactcgc gcctgaggtg ctttaaacgg tccgggagaa cgtgttcgtg cagattcgta
    13561 aaaatgacag tgttatggtg atttccggca aggagcgggg caagactggc aaagtcctcc
    13621 gtgtgaatcc gaaagaagat gccgtgatta tcgaaagaat caatgtagtc aagcggcaca
    13681 ctaaaccgcg aggccctcag caagccggcg gcatcattga aaaggaagcg tctatcccgg
    13741 gatcaaatat catgatcatg tgtgataaat gcaacgcgcc ggtgcgcatt ggccgaaaac
    13801 ttcttaccga tggcaaaaag gtgcgtgtct gtcgacgttg cggcgaggcg ttggattgat
    13861 ggtaactagg atggtacggc taaaacagaa atacgctgag caggtggtgt ccgcgatgaa
    13921 gtccgaattc ggttataaga attccatgca ggtgccgaaa ttggaaaaga tcaccatcaa
    13981 cgtcggtctt ggagaggcga cgcaaaatat caaagccttg gattctacgg tcgcggagat
    14041 tac
//
LOCUS       DASUVS010000154         5396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1372688, whole genome shotgun sequence.
ACCESSION   DASUVS010000154 DASUVS010000000
VERSION     DASUVS010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372688"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1927)
                     /locus_tag="VF182_08705"
     CDS             complement(149..1927)
                     /locus_tag="VF182_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437199.1"
                     /translation="METSRLEIGCAAIKYLVPAAHPAPLRVRDRLDRIIERELPRMLA
                     RAFDLWFSDNDPSLWIIRQLQIQAAVNVTGEPEQITRTVTTQIAKHLGATFQDENQDN
                     VRHFPSRAAYLASFLADLSFGTAKSQWYYQSFAGLNALPLSGALRTALCDNARIGKEA
                     LRLLSATELLRVLQSLTGQDARQVLEGIASEGALADASLCREVVVEAAMNNGAALNDL
                     ADEWQRALYLFTAAIDKTKGSDGAALKDMALKVAQDQDSWSKPIVVPGDRGQSYRRST
                     ALGGSFLLLPILDELPLAEATQDWPNTEEAAAISLVRFLVLLKCCGCHSSERAFYDPL
                     LRDLLLIPPSLSPEVLQTWQAQLKREHLERFLEALMDWQCSRAALGEKEQLLIISQYC
                     GDPLLVLIDAARGLWLLLDRYSAHHRREVTAALSAALARLMAEDGVLYSDPSLIDMLQ
                     TEFTGLNVIKLSDHTIDMAGAEKPAIDTILARLDKLPEELEFLRSPRSFEIARRLDLA
                     LSIVAQHLLRQFAYGLPGFAGSNLPYLSRNFFAFAVTVEEEPARRVVRLGHPPLHLIL
                     NMTGLNRQSYRLSWLDERRFALFQGE"
     gene            complement(1937..2161)
                     /locus_tag="VF182_08710"
     CDS             complement(1937..2161)
                     /locus_tag="VF182_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015132831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437200.1"
                     /translation="MKEQIKNRIESLRVEYRTGQEVLAELEGRQAGLKITLTRISGAI
                     QVLEELLEENNSTIVGTTQNAASTAQRAAA"
     gene            complement(2158..4395)
                     /locus_tag="VF182_08715"
     CDS             complement(2158..4395)
                     /locus_tag="VF182_08715"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEX6437201.1"
                     /translation="MPANQTQRPKFFEEQYLGAADLTAVVDYGRTQQARHALGAHTWG
                     IAIGLTLKETPQAGGAVTVHLLPGYGWDGYGRPIVVLSPYQIPEERFSAIKFDPSIDS
                     DGKGRLIPVWLCYDETATQSPRPGFEVCDVADQLSRIQESFRIEIGDQPTPADRYSGV
                     SVAAKNITDAATSLQAFDPAAPLVRDESIPQQSFPDPQNRPRWLIPIGYVRWLPVQNQ
                     AGHFVARDDSGAGGLPKDSDQIRIFRRYAGVVAETIEAAAGRIRLKNRGNGLPTVHSD
                     ELVWVEGDLRIEGDLRFFNHKLEFRDSGGSDNGAPLSINRVDGAGARLQVVIGKASAG
                     ANGFAVGPLDASNNFTAKLTVRDDGKVGIGTAAPALTLDVQGDFGRADGPATGHFWQS
                     QVGDVGGGILFLRSGGGVVAFDGNDAVGIGTAAPVAKLEVAGDIALEKIPPGTGRVLP
                     AEATLCWNDGTWLRLNQNLDFSKPVFGVHTPGVFAPGALNVGGAAGWGDPGFGNVWVT
                     GRIGIGTSAPQASLQIAGGAIMPAVGNGPSAGIQFPSDPGGGGGDEAFIRYFVQTGER
                     TKLLIGINNDADDSLGFHQFGGERMTIRGGNVGIGTTNPQTLLHVNGLASKPGGGTWT
                     NSASDARLKKNIEPLQGALNRMLQLRGVNYEWKDPASMGNLVGMQLGLIAQEVEPVFP
                     DWISTGRDGYKQLTIRGFEALTIEALRELKNEIEGMKKRLDKIEAGNAATGQRPRKEP
                     REKSS"
     gene            complement(4409..>5396)
                     /locus_tag="VF182_08720"
     CDS             complement(4409..>5396)
                     /locus_tag="VF182_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437202.1"
                     /translation="EQALGGMFQRFESCMKGLFEAVPALPTNIEQAQAWCCQLKANWI
                     EFFTDHPMHDCLALQKLSQFVCPAPDQTQTPEQYVQAVVVPLAPIVGEYIRSCLCSAL
                     LPPCPDPVASNCVPLATITIRKRDCKIMRVCNWSQRKFATTFPNLQYWFSFLPFTRLL
                     REAIESVCCRPLRGLEGGQFSVAARTDKRFTAFRRSAMEINPEQEFSRVLLDAIRNRA
                     RGIDAQTLFLGAMEFQDDKQRPLMTDAELRNPLQYLLLNQLAAPLVQNAVPEETAESL
                     KTALANLSRIVSAPEAATTDVQAFSSQLEELRRVVQDQQTRINELRERLGNR"
BASE COUNT         1105 a         1668 c         1497 g         1126 t
ORIGIN      
        1 gattgacctg ttcgtcactg atgtaaaggc cgcgaaactc gtcgagggat agttggtagc
       61 gcgcgcgcaa gcgcaggatc tcgcgatgga gcaacagatc gagccgctgc aactctaagg
      121 taaggtagtc cctcgggtcc attgccactt actcgccctg gaaaagcgca aaccggcgct
      181 catccagcca gctcaatcga tagctctgcc gattcagccc ggtcatgttt agaattaaat
      241 gcaacggcgg atgccctagg cgcacgaccc tgcgcgccgg ctcctcttcg acggtgacgg
      301 caaacgcgaa aaagttacga gacaaataag gtaggttcga tccggcaaaa cccggcaaac
      361 cgtaggcaaa ttggcgaagc agatgttggg cgacaatgct tagagccaga tcgagccggc
      421 gagcgatttc aaacgaccgg ggcgatctca gaaactccag ctcctccggc aatttgtcaa
      481 ggcgggcgag gatagtatcg atggccggtt tttctgcgcc agccatatcg atcgtgtgat
      541 ctgataattt gatgacgttc aacccagtaa attcagtttg aagcatgtca atcagactag
      601 gatcgctgta gagaacgccg tcctctgcca taagcctcgc gagtgcagca ctcagtgctg
      661 cggttacttc tcgccggtga tgcgctgagt atcgatcgag cagcagccac aatccgcggg
      721 ccgcatcgat caagaccaac aatggatcgc cacaatattg gctgatgatc agcagctgtt
      781 ctttttctcc aagagcagcg cgagagcatt gccaatccat gagagcctct aagaatcttt
      841 ccaggtgctc gcgcttcaat tgcgcttgcc aggtctgcaa cacttcaggc gacaggcttg
      901 gaggaatcaa cagtaaatcg cgcagcaacg gatcgtaaaa agcgcgttcc gagctgtgac
      961 agccgcagca tttgagcaaa acaagaaacc tcaccagcga tattgccgcc gcctcttcgg
     1021 tattcggcca gtcctgggtc gcctccgcca gcggcaactc atccagaatc ggtaagagca
     1081 aaaaacttcc gccaagtgcg gtgctccgcc gatacgactg acctcggtca ccaggaacga
     1141 cgatcggttt cgaccaacta tcctgatctt gcgcgacctt gagagccatg tccttcagcg
     1201 cggcaccgtc tgatcctttt gtcttgtcga tcgcggcggt gaataggtac aaagcacgct
     1261 gccattcatc tgcgaggtcg ttcaacgccg cgccgttgtt cattgctgct tcaacgacta
     1321 cctcacggca aagagaggca tccgccaacg cgccttcgga tgctatccct tcaagcacct
     1381 gccgcgcgtc ttggccggtc aagctctgta ggacccgcag cagctcagtc gccgataaaa
     1441 gacgcagcgc ctccttgccg atccgagcgt tgtcgcatag cgccgttcgc aaagcgcccg
     1501 acaggggcag cgcattcagt ccggcgaacg attggtagta ccactggctc tttgccgttc
     1561 cgaacgacaa atccgctaga aagctcgcca gataagcggc acggcttgga aaatgacgga
     1621 cattatcctg attttcatcc tgaaatgtcg ctcctaaatg ttttgcaatc tgagtcgtca
     1681 ctgtgcgagt gatctgctcc ggctcgcccg tgacattgac ggcggcttga atctggagtt
     1741 gtcgaataat ccagagactc ggatcattat cggagaacca caagtcaaac gcgcgcgcga
     1801 gcatgcgcgg aagttcgcgc tcgattatcc ggtcgagccg atcgcgaacg cgcagcggcg
     1861 ccggatgcgc cgccggcaca agatacttga tcgccgcaca accaatctcg agccggctcg
     1921 tctccactgc gaaatcctag gccgcggccc tttgcgccgt actcgccgcg ttctgtgtgg
     1981 tccccacgat ggtcgaatta ttttcctcga gcagttcttc aagaacctgg atagcgccgc
     2041 tgatgcgagt cagggtgatc ttgagcccgg cctgccgtcc ctcgagctcg gcgagcactt
     2101 cctggccggt ccggtattcc acccgtaacg attcaatgcg atttttgatt tgttctttca
     2161 tgacgatttc tccctgggtt ccttcctcgg tcgctgaccc gtcgcggcat tccccgcttc
     2221 aattttgtcg agccgctttt tcatcccttc gatctcattc ttcagttcgc gcagagcttc
     2281 gatcgtcaac gcttcaaatc cacggattgt cagctgctta tagccgtccc tgccagtgct
     2341 gatccaatca ggaaagacgg gctccacttc ctgggcgatc aagcccagct gcataccaac
     2401 gagattgccc atgctcgcgg ggtccttcca ttcatagttg acaccgcgta gttgcaacat
     2461 tctgttcaaa gcgccttgca gaggttcgat attttttttc agtctcgcat cagaggccga
     2521 attcgtccag gtaccaccgc cgggcttgct cgcgaggcca ttgacgtgca gcagagtttg
     2581 cgggtttgtc gtgccgatgc cgacgttgcc accgcgaatc gtcatgcgct caccgccgaa
     2641 ctggtggaaa ccgaggctgt catcggcatc gttattgatc ccgatcaata gtttggttct
     2701 ctctcccgtc tggacaaagt aacggatgaa cgcttcgtcg ccgccgcccc cgccggggtc
     2761 ggatggaaac tgaatcccgg cgctcggccc gttgccgacc gccggcatga ttgcgccgcc
     2821 ggcaatctgc agcgaggctt gcggtgcact cgtgccgatg ccaattcggc cggtaaccca
     2881 gacattgccg aaacccggat ctccccagcc ggccgcgcca ccgacgttga gcgcgccagg
     2941 cgcaaagact ccgggcgtat ggacgccgaa cacgggcttg ctgaagtcaa ggttctggtt
     3001 caggcgcagc caggtgccgt cattccagca cagggtcgct tcggcgggaa gaactcttcc
     3061 cgtccccggt ggaatttttt ccagcgcgat atcgcctgcg acctcgagct tggctaccgg
     3121 cgccgcggtg ccgatgccga cagcatcatt gccgtcgaac gccaccacgc caccgccact
     3181 gcgcaagaaa agaatgccgc cgccgacatc gccgacctgt gattgccaga agtgaccggt
     3241 agcggggccg tcggcgcggc caaaatcccc ctgcacgtcc aaggttagcg ccggcgcagc
     3301 ggtgccaatg ccgaccttgc cgtcatcgcg caccgtcagc ttcgcggtaa agttattgct
     3361 ggcatcgagc ggcccgacgg caaaaccatt cgcccccgcg ctcgctttgc cgattacgac
     3421 ctgcaagcgc gcgccggccc catccacgcg attgatcgac agcggcgcgc cattgtcgct
     3481 accgccgctg tccctgaatt caagcttgtg attaaaaaac ctgagatcgc cttcgatccg
     3541 caaatcgcct tcgacccaca ccagttcgtc cgagtgcacg gttggaagcc cgttaccgcg
     3601 atttttgagc cggattcggc cggccgccgc ttcgatggtt tcggcaacga caccggcata
     3661 ccggcggaag atgcggatct gatccgaatc cttcggcagg ccgcccgcgc cgctgtcgtc
     3721 gcgcgcgaca aaatgcccgg cctggttctg caccggcagc cagcgcacat agccgatcgg
     3781 gatcaaccag cgcgggcgat tctgtggatc gggaaaactc tgctgcggaa tcgattcgtc
     3841 gcgcaccagc ggggccgccg gatcgaacgc ctgcagcgaa gtcgcggcat cggtgatatt
     3901 tttcgccgcg acgctcactc ccgagtagcg atccgccgga gtcggctgat caccgatctc
     3961 gatgcgaaaa ctttcctgga tgcgcgacag ttgatcggcc acgtcgcata cttcgaaccc
     4021 gggacgcggg ctctgggtcg cggtttcgtc atagcagagc cacaccggga tcaggcgccc
     4081 cttgccgtcg ctatcaatgc tcggatcgaa cttgatcgcg ctgaatctct cctcgggaat
     4141 ctgataaggc gaaagcacca cgatcggccg gccgtaaccg tcccagccgt aacccggcaa
     4201 caagtggacg gtcaccgcac cgccggcctg aggcgtctct tttaaggtca agccgatggc
     4261 gattccccag gtatgcgcgc cgagcgcgtg gcgcgcctgc tgagtgcgcc cataatcgac
     4321 gacggccgtt aagtcggcag cgccgagata ttgctcttcg aaaaacttcg gacgctgcgt
     4381 ctggttcgcc ggcatggtga tcctccgttt agcggttgcc taagcgctct cgcaactcgt
     4441 tgatccgggt ctgctgatcc tggaccactc gcctcaattc ttcgagctgg ctgctaaacg
     4501 cttgcacatc cgttgtggcg gcctcaggcg cgctgacaat tcgtgaaaga tttgctaacg
     4561 ccgtttttag tgattccgcg gtttcttccg ggacggcatt ttgaacgagc ggcgccgcaa
     4621 gttgattgag aagcaagtac tggagcggat tacgcagctc ggcatctgtc atgagcggac
     4681 gctgcttatc atcctggaac tccatcgcgc cgagaaagag cgtctgcgca tcgattccgc
     4741 gggcgcgatt tcgtatagcg tccaacagca cccgggagaa ttcctgctcg ggattgattt
     4801 ccatggcgct tctcctgaac gcggtaaatc gtttgtccgt tcttgcggcc acggagaact
     4861 gtccgccttc aagacctcgt aatggcctgc agcagacgct ctcgatcgct tctcgcaata
     4921 ggcgcgtgaa gggcaagaat gaaaaccaat actgtagatt gggaaatgtg gtcgcgaact
     4981 tccgctggct ccaattgcac acccgcatta ttttgcaatc ccgcttgcga atcgtgattg
     5041 ttgccagcgg cacgcaattg ctggcgactg gatcgggaca gggaggcaac aacgccgagc
     5101 acaggcacga acggatatac tcgccgacaa tcggcgccag cggcacgacc accgcctgca
     5161 cgtattgctc cggcgtctga gtctgatcag gcgcgggaca gacaaactgc gacagtttct
     5221 gcagcgccag gcaatcatgc atcggatgat cggtgaagaa ctcgatccaa ttcgccttca
     5281 gttggcagca ccatgcttgc gcctgctcga tgttcgtggg aagcgccggc acggcctcga
     5341 acagaccctt catgcagctc tcgaaccgtt gaaacatccc gccgagagcc tgttcg
//
LOCUS       DASUVS010000155         5839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_61416, whole genome shotgun sequence.
ACCESSION   DASUVS010000155 DASUVS010000000
VERSION     DASUVS010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61416"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..448)
                     /gene="ssrA"
                     /locus_tag="VF182_08725"
     tmRNA           complement(99..448)
                     /gene="ssrA"
                     /locus_tag="VF182_08725"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(510..980)
                     /gene="smpB"
                     /locus_tag="VF182_08730"
     CDS             complement(510..980)
                     /gene="smpB"
                     /locus_tag="VF182_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX6437203.1"
                     /translation="MAKERATGEQNVCINRQARHNYFIEETYEAGLMLLGSEVKSLRD
                     GKANLTDSYARIQKGEAFLVNAHVSHYPGANQFNHEPTRTRKLLLHAREIERLTGKTK
                     ERGLTLVPLRLYFKDGKAKVELGLARGKKLYDKRETLRRKVAERDVERSLKARK"
     gene            complement(973..1626)
                     /locus_tag="VF182_08735"
     CDS             complement(973..1626)
                     /locus_tag="VF182_08735"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX6437204.1"
                     /translation="MGKDVPGKTRVRLRENFQRYFLAGLLVFLPVVITLWFLGWVIGL
                     LDGLLDVLPAGVHPNTYLPFAIPGLGALVTVTLILFLGILTRGVATRRFLAAWENIFI
                     KIPIFRGVYTAVQKLVQTFLGQSQNSRQVVMIEYPRKGIYTVGFAMGRAWKELERTQE
                     AQLVNVFIPTTPNPTSGFYLLVPTTEISALDMTMEEALKLITSGGLITPDERTRRRG"
     gene            complement(1598..2485)
                     /gene="panC"
                     /locus_tag="VF182_08740"
     CDS             complement(1598..2485)
                     /gene="panC"
                     /locus_tag="VF182_08740"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX6437205.1"
                     /translation="MRIIRNIAEMTDWSQAERQRGHRIAFVPTMGALHEGHLSLVRDA
                     GMRADRVVVSIFVNPTQFAPAEDFAAYPRNFARDKALLEAEAIDVLFHPVAEDIYPPG
                     NQTYVEVKQLSNVLCGAQRPGHFQGVATVVTKLFNVVRPDVAIFGEKDYQQLQIIRRL
                     VRDLLMDIEIVAHPIVRESDGVAMSSRNAYLNIEQRKAARCLSRSLHSAECLVRQGEA
                     AAKTIAEAVVAELHNEPLAVLEYVAICDVETLEPLEQINHSALLALAVTIGKARLIDN
                     KILLRSPGAQGGERCAGKD"
     gene            complement(2519..3319)
                     /gene="panB"
                     /locus_tag="VF182_08745"
     CDS             complement(2519..3319)
                     /gene="panB"
                     /locus_tag="VF182_08745"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX6437206.1"
                     /translation="MAEKVTVPGIVKMKQQGEKITCLTAYDYTLARILDDAGVDVLLV
                     GDSVGTVVQGQPNTLAVTMDQMIYHTRIVARGRKRALVVGDMPFLSFQAGREQALRNA
                     GRFLQEADAEAVKLEGGVFMRDTIAAIVNAGIPVMGHVGLTPQSLHRFGGYKVQGREK
                     SQHESIVRDAVAVQEAGAFAIVLEGVPTALAKEITERVTIPTIGIGAGIHCDGQVLVV
                     HDMLGLFDDFTPKFVKRYADIKLSMAAAVRDFIDEVRDRKFPGEEHSF"
     gene            complement(3435..4076)
                     /locus_tag="VF182_08750"
     CDS             complement(3435..4076)
                     /locus_tag="VF182_08750"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEX6437207.1"
                     /translation="MSNTKYIVVEGPIGVGKSSLAKMLANEFQGRSLFERIEDNPFLP
                     KFYKARQTYAFQNQTFFLLNRYQQQMELSQQDLFNQNIIADYLFAKDRIFATLTLSAE
                     ELNLYQQIYSLLNARVPKPDLVVYLQARPEVLYKRVKKRDKKYERGVTFEYLNEVAQA
                     YNQFFFHYDETPLLVVNTSKIDFVESSKDLADLIKEINNMGSGTQHFIPLGSR"
     gene            4140..5213
                     /gene="nagZ"
                     /locus_tag="VF182_08755"
     CDS             4140..5213
                     /gene="nagZ"
                     /locus_tag="VF182_08755"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX6437208.1"
                     /translation="MKQLESHVGQMLMVGIDGYELTTAEVKICRSLNFGGFILFNRNC
                     CEPAQINSLCRALATATRNLPFIAIDEEGGRVHRLPWPFTHFPPAELVGKTANPDLAF
                     RVGLAVAAELTLLGINLNFAPVLDVNSNAANPIIGDRSFGAAPNQVAAMALAWSEGLS
                     TGGIVPCGKHFPGHGNTDRDSHLDLPVVAKSLGELQEVELKPFIRACKSPIEALMTAH
                     VLFPALDRELPATLSRRIVTGVLRKELGYEGVVFSDDMEMGAIAGNFDREEAALLAVG
                     AGIDVLLYCHDMAKAVGIFELLCRKARQDSHLRAKINESCVRIGKLKERCLKEHRGVP
                     DNQVVERLLALNHRKLIDEVHGS"
     gene            complement(5201..>5839)
                     /locus_tag="VF182_08760"
     CDS             complement(5201..>5839)
                     /locus_tag="VF182_08760"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HEX6437209.1"
                     /translation="FAFSPDVSFAHYVEYALDVPMYFIIRNKNYIDMTGTTFRQYLAY
                     GWNGERATLEDWHDHLTTLFPETRIKRYIEVRSADSQPPELMPALSAIVKGAFYESDC
                     LDAAWDLVKDWTWDERMQVYLDSHRDALAARIRRHSLLDLSKELLQIALEGLRRQKMF
                     NALGEDETIYLEPLDKLLALGKCPADLLIEKWQGELDQDIRKLIAYSAYQLP"
BASE COUNT         1345 a         1598 c         1486 g         1410 t
ORIGIN      
        1 gcgtaaccat cagtcgctgt tcatcctgaa ttgatatgca gcgcagccga tcggctgcgc
       61 tgcattttta atagaccgcc cgtcggcgga ttagcatttg gtggaggcgg ggggaatcga
      121 acccccgtcc gagagtcttc catctccgcg actacatgct tagccgatgt tttaattcac
      181 ccctggaacc cccaacggca gggtttcccg gggccatccg gtttagatct cagcctcatt
      241 ccctccggca gggaaatcgg cccatcccac tgaatgacgt cttatccgag ctcgtgggag
      301 aagatcggta agacgctaac tgcggttagg cagctagttg gtattcgaaa ttatctgcgt
      361 ttaaattgca gtctgtttgt ttaacgggac aacagtaccc cggcatgcca ctttgacttc
      421 ccacccccgt cgaacccata tcgcccccat acagacagct taccgttttg gggaccaagt
      481 tgcgagagca gctcccgggt cgccaatcac tattttctgg cttttagcga acgctcaacg
      541 tctcgttcag ccactttccg acgcagtgtc tctcgcttgt cgtaaagctt tttgccacgc
      601 gccagtccca actcgacttt cgccttacca tccttgaaat ataatctcaa aggcaccagg
      661 gtcaaccctc gttccttggt tttgcctgtt agcctttcta tctcgcgcgc atgtagaagc
      721 agctttcggg tgcgcgtcgg ctcgtgatta aactggttgg ctcccgggta gtggctgaca
      781 tgagcattga ctaagaaggc ttcgcccttc tgaatgcgcg cgtaactatc agttagattg
      841 gccttgccat cgcgcagcga tttcacctca ctgccgagca acatcagacc ggcctcgtaa
      901 gtctcctcta taaagtaatt gtgccgagcc tgccgattga tgcaaacgtt ttgctcgcct
      961 gttgctcttt ccttagccac gtcgcctggt cctctcgtct ggagtaatta agccgcccga
     1021 agtgatgagc ttaagggctt cctccatcgt catatcgagt gctgatattt cggtcgtcgg
     1081 cacgagaagg taaaatccgg acgttggatt aggcgttgtc gggatgaaaa cgttgacgag
     1141 ctgagcctct tgcgtccttt caagctcttt ccatgcgcgc cccatggcaa atccgacggt
     1201 atagatccct ttgcgtgggt actcgatcat cactacttgg cgcgagttct gtgattgccc
     1261 caagaaagtt tgcacaagtt tctggacggc tgtatagacg ccacggaaga tcgggatttt
     1321 tatgaagata ttctcccatg ccgcgaggaa cctgcgtgtg gccacgcctc gggtcagaat
     1381 cccgagaaat aagatcaacg tcaccgtgac gagtgcgcct aatccaggga ttgcaaaagg
     1441 taaataggta ttcgggtgga cgccagccgg taagacgtca agcagtccat ccaacagccc
     1501 gataacccac cccagaaacc acagagtgat gactacgggc agaaaaacca gaaggccggc
     1561 taaaaaataa cgctgaaagt tctctcgcaa tcggactcta gtctttcccg gcacatcttt
     1621 ccccaccttg tgctcctgga gatcgcagca aaattttatt atcgataagg cgcgctttgc
     1681 cgatggtgac ggccaacgcc agaagcgcgg aatgattaat ctgctcgagc ggctctaaag
     1741 tttcaacatc gcagatggcc acgtattcga gcaccgccaa gggctcattg tgcagctctg
     1801 ccacgaccgc ttcggcaatg gtttttgccg ccgcttcccc ctggcggacc agacattcgg
     1861 cgctgtgaag cgagcgtgac aaacagcgcg ccgcctttct ctgctcgata ttaaggtacg
     1921 cgttgcgcga actcatggca acgccgtcgc tttcacgcac gatcggatgg gcaacgattt
     1981 caatgtccat taagagatcg cgcaccaagc gccgaatgat ctgcaactgc tggtaatcct
     2041 tctctccaaa gatcgccacg tcagggcgaa caacattgaa aagcttggtc acgacagttg
     2101 ccacaccctg gaaatgcccc gggcgctgcg ctccgcataa gacattgcta agctgtttga
     2161 cttcaacgta cgtttgattg cctggaggat aaatgtcttc cgccaccggg tggaaaagta
     2221 cgtcgattgc ctccgcctcg agcagagcct tgtcgcgggc gaagtttcgc ggataagccg
     2281 caaaatcttc cgccggagca aactgcgtcg ggttgacgaa aatagagacc acgactcggt
     2341 ctgctctcat tccggcgtca cgcaccaggc ttaagtgccc ttcgtggagg gcgcccatag
     2401 tcggtacgaa ggcgatgcgg tggcctcgct ggcgctcagc ttggctccaa tcggtcattt
     2461 cagcaatgtt ccggattatc cgcatcgtac atatcgggtg actggatcag ctgtttactc
     2521 aaaaggaatg ctcctcgcca ggaaatttcc gatctcgaac ttcgtcgatg aaatctctga
     2581 ccgcggcagc cattgaaagt ttaatatcgg catagcgttt gacgaacttg ggggtgaagt
     2641 catcgaacag accgagcata tcatgaacta ctagaacctg tccgtcgcag tgaataccgg
     2701 cgccgatgcc aatggtagga atggtcacgc gttcggtgat ttcctttgcg agcgcggtcg
     2761 gaacgccttc aagcacaatt gcgaaagcac ccgcctcctg gacagccaca gcatcacgca
     2821 cgatactctc atgttgtgat ttttcgcgac cctgaacctt atagccgccg aaccgatgta
     2881 gcgactgggg cgttaacccg acgtgtccca tcactggaat tccggcgttg acgatggctg
     2941 cgatcgtatc ccgcatgaag actccgcctt caagtttgac cgcctccgca tccgcttcct
     3001 gaagaaatcg tcccgcattg cgcagcgcct gttccctgcc cgcttgaaac gacaagaagg
     3061 gcatatcacc gacgacaaga gcgcgtttgc gaccccgcgc cactatccgg gtatgataaa
     3121 tcatctgatc catcgtcacg gctaaggtat tgggttgccc ttgaacaacg gtgccaacag
     3181 agtccccgac caagagaacg tcgaccccag catcgtccag aattcgcgca agcgtatagt
     3241 cgtaggcagt aaggcaggta attttttcgc cttgctgttt catcttgacg atgcccggca
     3301 cggtgacctt ttccgccatg gctaccctcc caaaaaaaaa gccttccaga caagcccgga
     3361 aggccaatgt gaccggaaag cctccgaagg ctctccgcac tcgctactcg gttttggcgt
     3421 ccgtctcagt ccaattatct ggatccaagc ggtataaaat gctgcgtccc cgaacccata
     3481 ttgttgattt ccttgatcaa gtcagccaga tccttgctgc tctcaacgaa atcaatcttt
     3541 gacgtgttca ccactaaaag tggcgtttcg tcataatgga agaagaactg gttataagcc
     3601 tgagctacct cgtttaggta ttcgaaagtc acgccgcgct cgtatttttt atcccttttt
     3661 tttactctct tgtaaagtac ttcgggacgc gcttgcaggt agaccaccag atcaggtttt
     3721 gggacccgtg cattcaagag agagtaaatt tgctgataca ggttcaattc ttcggcgctc
     3781 aacgtcaggg tcgcgaaaat tctatcttta gcgaacaagt aatcggcgat aatattctgg
     3841 ttaaagagat cttgctggct taactccatc tgctgctgat agcggttgag caagaaaaat
     3901 gtctgatttt gaaacgcata ggtttgccgc gccttataaa atttcggcaa aaacggattg
     3961 tcttcaatgc gctcgaacag ggatcgaccc tgaaattcat tggcgagcat cttggccaag
     4021 ctgctcttgc ctacgccgat tggtccttcg accacaatgt acttggtatt gctcatggaa
     4081 gtctgcgaat ataacacaat ccatcaaaaa tcgtccaccg tatgatagag ttggcgatcg
     4141 tgaaacaact agagagccac gtcggtcaga tgctgatggt gggtattgac ggttatgagc
     4201 tgacaaccgc cgaagtgaag atctgccggt ctctgaattt tggcggattt attctcttta
     4261 accgcaattg ctgcgaacca gcgcaaatta attctttgtg tcgcgcgctt gctacagcta
     4321 cccgcaatct tccgtttatc gcgatcgatg aagaaggcgg cagagttcac cgcctgcctt
     4381 ggcctttcac ccattttcca cccgccgagc tggtcggcaa aacagccaat cccgacctcg
     4441 cctttagagt tggtctcgct gtcgccgcgg agcttactct gctcggtatc aatcttaatt
     4501 ttgctccagt gttggatgtt aattccaatg ccgcaaaccc aatcatcggc gaccgctctt
     4561 tcggggcagc accgaaccag gtagcggcaa tggcgctggc ctggagcgaa ggcctaagca
     4621 ccggcggcat agtcccgtgc ggcaagcatt tccctggtca cggcaataca gatagggact
     4681 cgcacctcga cctccctgtg gtagcgaaat cactgggcga actgcaggaa gtggagttga
     4741 aacctttcat ccgcgcctgc aaaagtccaa tcgaagcctt gatgaccgct catgtacttt
     4801 ttccggcgct ggacagagaa ctccccgcga cgctttctcg gagaatcgtc accggagttt
     4861 tgcgcaaaga gctcggttat gagggagtcg tgtttagcga tgacatggag atgggcgcga
     4921 ttgcgggaaa cttcgatagg gaggaagcag ctcttttagc tgtgggcgcc gggatcgatg
     4981 tgcttctcta ctgccatgat atggcaaaag ccgttggcat ctttgagctg ctttgccgta
     5041 aagcgaggca ggatagtcac ttgcgcgcta aaatcaacga aagttgcgtg cgcatcggta
     5101 aactgaaaga gcgctgttta aaggaacatc gaggcgtgcc ggataatcaa gtggtcgagc
     5161 gactgcttgc ccttaatcac agaaaactga tcgatgaagt tcacggaagt tgatacgcgc
     5221 tataagcgat taacttccgg atgtcttgat ccaactcgcc ttgccatttt tcaataagca
     5281 gatcggcggg acatttcccg agcgctagaa gtttgtcgag cggttcaagg tagatagtct
     5341 catcctcgcc taacgcattg aacatcttct ggcgtctcag accttccaag gcgatctgta
     5401 acagctcttt ggacaaatcc aacagtgagt ggcggcgaat gcgcgctgcc aacgcgtcgc
     5461 gatgtgaatc gagatacacc tgcattcgtt catcccaggt ccagtccttg actagatccc
     5521 aagcggcgtc taaacagtcg gattcgtaga atgcaccctt gactatggct gaaagcgccg
     5581 gcatcagttc cggaggctgg ctgtcagcgg accgcacctc gatatatcgt ttgatccggg
     5641 tttcaggaaa caacgttgtg agatgatcat gccagtcttc cagggtcgcg cgctcgccat
     5701 tccacccgta cgccaagtat tggcgaaacg ttgtgccggt catatcgatg tagttcttgt
     5761 tgcgaatgat aaagtacatc ggcacgtcca gcgcgtattc gacgtagtgc gcgaacgaaa
     5821 cgtctggcga gaatgcgaa
//
LOCUS       DASUVS010000156         4942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1591908, whole genome shotgun sequence.
ACCESSION   DASUVS010000156 DASUVS010000000
VERSION     DASUVS010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591908"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VF182_08765"
     CDS             complement(<1..251)
                     /locus_tag="VF182_08765"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437210.1"
                     /translation="MKVLFICYANVGRSQVAETCFKSMSKHDCESAGIAVNERLAAMK
                     LASRKLKDNPNQSSLRYIRRELGRDIGEKEKQQLIPEMIT"
     gene            complement(498..1478)
                     /locus_tag="VF182_08770"
     CDS             complement(498..1478)
                     /locus_tag="VF182_08770"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6437211.1"
                     /translation="MKIDVHAHLFSPAYIRRLERIFGNDNSPAGQDAMRVIRWMNSDP
                     RMTDVEGRLEEMARFKIDMQVLSVPFHGALVKDRAAAAELTQLANDVLVEAARSHPQH
                     FRALLVMPLQFPELAIEELDRFSGRAEVVGVIVAGSAGGRQLNDSAFMPFYAELERRG
                     LPFLLHPISPPGLDCMLELNLANVVGFMFETTLAAVRLVYAGVFERYPGLHMIFPHLG
                     GLAPYLMGRVQWGYERFPPCSENISNPPEFYFKRFYYDTVCRNVPALRMALSMFGIKQ
                     IMFGTDIPLREDIELQLRDIDELGLSAAERDALFAGNAARLLGVSLPGDL"
     gene            complement(1612..2391)
                     /locus_tag="VF182_08775"
     CDS             complement(1612..2391)
                     /locus_tag="VF182_08775"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6437212.1"
                     /translation="MFEVAEAYEQRMGRWSRQLAPLFADFVGVRDGEKVLDAGCGTGS
                     LSATLAKVTGASEIVGIDPSKGFIEYARMQVADPRVIFEVADAQDLHYPDHTFDVCVA
                     LLAVDHIPDAPKATKEMRRVTKTLGVVATAMWDRSHDNELYGCFWDAAEATDPTAKRP
                     SERRKSYGSPEALSQLWNSAGLTEIKFTALSMACQFSSFDELWQRCLDGDSPSGVHMK
                     RLSADGHEVLRKKLRQNVLGNGLDGPFALKAKAWAVRGTVP"
     gene            complement(2824..3348)
                     /locus_tag="VF182_08780"
     CDS             complement(2824..3348)
                     /locus_tag="VF182_08780"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEX6437213.1"
                     /translation="MNNAMLKIIIVSGVLAVVRLADAQQAIFLVRHAEDVRSKDVVDR
                     PLTESGHRRATLLAGVLKDAGINAIFTSSLQRAVKTAEPLAKAANIESKPLPQLTTKF
                     EPKDMESFVELLRTEHRESIVLFVGHSNTVPALIKALGHMAEIKIPETEYDNLFLVFP
                     KSEGPPTVLRLRFN"
     gene            complement(3371..3790)
                     /locus_tag="VF182_08785"
     CDS             complement(3371..3790)
                     /locus_tag="VF182_08785"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6437214.1"
                     /translation="MSSNPSEWTVFMERLEAAEQEFVQGHPAEFQALWSRTDDVTLCG
                     GFGGVERGWQNVISRLDWVSTKYADGSRTSEEISSVVGPDFAYLVQTEVIRARIAGQA
                     HFSIQELRATMLFRREQEGWRIVHRHADSQMLTRPPK"
     gene            complement(3946..4410)
                     /locus_tag="VF182_08790"
     CDS             complement(3946..4410)
                     /locus_tag="VF182_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6437215.1"
                     /translation="MILPLPEANSEISAEVREWLDRFAVCVREVDYAAARPFWHPDIV
                     IFGTYQELVKSRSAWTERQWDNVWPRTAEFRFDLANTMVMAAPDGAMAVAIAPWTSTG
                     FHEDGTAFYRPGRATIVLARQPDGRWLGVHSHMSLARGVPQESHGKRPVKAR"
     gene            complement(4486..>4942)
                     /locus_tag="VF182_08795"
     CDS             complement(4486..>4942)
                     /locus_tag="VF182_08795"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6437216.1"
                     /translation="DLPLAIDNARTFVRERATKHGITLDVAIDERLGDFIGDERKIKQ
                     ILLNLLSNAVKFTPEGGRIEIAARQNNGSVEISVTDSGIGIAPEDQPMIFEEFRQVGS
                     DYEHKREGTGLGLTLAKKFVELHGGKIWLTSEAGKGSSFTFSLPITAAL"
BASE COUNT         1118 a         1417 c         1245 g         1162 t
ORIGIN      
        1 gtaatcattt cagggatcag ttgttgcttt tctttttcgc cgatgtctct acccaactct
       61 cttctgatgt agcgcagtga actctggttg ggattgtctt tgagttttct gctcgcaagc
      121 ttcatggccg cgagcctctc attgaccgcg attcccgcgc tttcgcaatc atgcttcgac
      181 atgctcttga agcaagtttc agctacctgg cttcgtccga cattggcata acagataaag
      241 agaactttca tcgctgttcc tggttattcg taagttgagt ctatgaatgt ccgtctcaac
      301 aaatgggtct acgtaccaaa cttgctcccg acgtttcgaa gttatcgatc ctgtggtgtg
      361 gccgatcgta actccaatct gccaccgcag gattcatcag cgaagatcaa tgttcgctgc
      421 gaagcgcggg tctatttgtt tcaatgagcc tgtcatgatt gccaggatga tgagctccag
      481 agtcctccat ctcttgatta taaatcaccc ggcagcgaga cgcctagaag acgcgctgcg
      541 ttcccggcga ataaagcatc ccgctccgca gcgcttaggc ccagttcatc aatatcgcgg
      601 agctgcaact cgatatcttc ccgcaggggg atgtcggtgc cgaacatgat ctgttttatc
      661 ccaaacatcg acagggccat gcggagcgcc ggcacattcc ggcagacagt gtcgtaataa
      721 aagcgcttga agtagaactc gggcggattg ctaatattct cactgcatgg tggaaatcgc
      781 tcgtatcccc attgcacccg acccatcagg tacggcgcaa gcccgcccaa gtgagggaag
      841 atcatatgga gccccggata acgttcgaag actccggcgt aaaccagccg cacggcagcg
      901 agggtagtct cgaacatgaa tccgacgacg ttggctaggt tcaactcgag catgcaatcg
      961 agccctggcg gcgagatcgg gtgcagcaga aacggtaaac cgcgccgttc gagctcggca
     1021 taaaacggca tgaaagccga gtcgttcaat tggcgtccgc cggcgctgcc tgcgacaatc
     1081 acgccgacaa cttccgctcg gccagagaaa cggtccaact cctctatggc gagctcagga
     1141 aattgaaggg gcattaccag cagcgcacgg aaatgttgcg ggtggctgcg ggcggcctcg
     1201 accagcacat cattggctaa ctgtgtcagt tcagcggccg ccgcccgatc ctttaccagc
     1261 gcgccgtgaa atggaacaga aagaacctgc atgtcgatct taaatctggc catctcctcc
     1321 agccggccct ccacatcggt catacgcgga tcactgttca tccagcggat caccctcatt
     1381 gcgtcctgcc cggccggcga gttgtcgttg ccgaagatcc gctcaagtct tcgaatgtaa
     1441 gcgggtgaaa aaagatgggc atgcacatcg attttcataa tgaacctcga ataggttaga
     1501 agttactagt ttgccttaca gccgaaaaaa taaacccgcg catcagcagc gtgagcttca
     1561 gccgtgtgac gcatacttcg gaacgatctt ctgcggcata cacaattcgt tctaaggcac
     1621 cgtgcctcgc acggcccagg ctttggcctt gagggcaaat ggcccgtcga gaccattacc
     1681 caaaacattc tgtcgtagct tttttctcaa cacctcatga ccatctgccg atagacgctt
     1741 catgtgtaca cccgaaggac tgtcgccatc caaacaacgt tgccagagtt catcaaatga
     1801 agaaaactgg cacgccatcg acaaggctgt gaatttgatt tcggtcaaac cagcgctatt
     1861 ccatagctgt gaaagtgctt cgggagaacc gtacgatttc cgcctctcag agggccgttt
     1921 cgctgtcgga tcggtcgctt cggcggcatc ccaaaagcag ccgtaaagct cattatcgtg
     1981 gctacgatcc cacatggcgg ttgcgacgac acccagtgtt ttggtaacgc ggcgcatttc
     2041 tttcgtggcc ttcggggcat ccggaatgtg atcaaccgcg agcagagcca cgcatacgtc
     2101 gaaagtgtgg tctgggtaat gcaaatcttg cgcgtcggcg acttcaaaga ttacccgagg
     2161 atcggcaact tgcattcgcg catattcaat gaacccctta gaaggatcta tgccgacgat
     2221 ctccgacgct cccgtgactt tcgccagagt cgctgaaagc gatcccgtcc cacagccagc
     2281 atcgagcacc ttctcaccat cccgaacacc gacaaagtca gcaaacaaag gggccaactg
     2341 gcggctccag cgccccatcc tctgttcgta agcttcagcg acttcaaaca ttagatcctc
     2401 ctgttttttc ctatgcccct gcgctcattg ctcagagata gttttacaga ccgctaaaag
     2461 atggattaac cgcatgataa atttggacgc cgctcaattt gaatgaatga ccagactcct
     2521 ttatcagcca aacactttcg ttcagatctt cccgattaga tcgactttcg cgccgttcag
     2581 accgcgtgcg tcatccagta aaagattgct gcgctcaata tcaataaatt ccccggtcag
     2641 ttaacaagtc cgaggcgttc tttctcggtg tgagattcgc ccgcatcgta atggcgctaa
     2701 tctcaatttg ttgatcgtat cgagacgagg agttgaatct tccatacggt cttccttgtc
     2761 agaattgtat gatggaagag tgatcgtgtt ccgaatgaag caggagcact gatgatgaac
     2821 agcttagtta aagcgaagac gcaaaaccgt cggaggacct tcgctcttgg gaaataccag
     2881 aaacaagttg tcgtactctg tttcagggat ttttatctcg gccatatgtc ccaacgcctt
     2941 gattaaagcg gggacggtat tcgagtggcc gacgaacaaa acgatacttt cgcgatgctc
     3001 agtccggagg agctcaacga aactctccat atccttcggt tcaaactttg tcgtgagttg
     3061 cgggagcggc ttcgattcga tgtttgcagc tttcgctaac ggctccgctg tcttaaccgc
     3121 gcgctggaga ctgctcgtaa agatcgcgtt gataccagca tccttcagaa cacccgcgag
     3181 cagcgtcgcc cgtcggtgtc cggattcggt taaaggacgg tcgaccacgt ccttggatct
     3241 cacatcttct gcgtgccgaa ccagaaagat tgcctgttgc gcgtcggcaa gtcggacaac
     3301 agcgagaacg ccgctaacga ttatgatctt gagcattgca ttgttcatac gtctccatcc
     3361 gaaatggtta ctattttggc ggccgcgtaa gcatttgcga atccgcgtgt cggtgcacga
     3421 tccgccatcc ttcttgctcg cgccgaaata gcatcgtggc gcgtagctcc tggattgaaa
     3481 agtgagcctg accggctata cgcgcgcgaa tcacttccgt ctgaacaaga tacgcgaagt
     3541 cgggaccaac cacgctggat atttcttcac ttgttcgact cccatccgca tactttgtgc
     3601 ttacccagtc taggcgggag attacgttct gccagccacg ttcgacgccg ccgaagccgc
     3661 cgcagagggt aacgtcatcg gtccgagacc atagagcttg gaactcggca ggatgacctt
     3721 ggacaaactc ctgttcggcg gcctcaagac gctccataaa gaccgtccat tcagatggat
     3781 tgcttgacat cacagacctt cactttattt gatcaccctg tccgtgcgcg gcaggcggaa
     3841 tcgtcagatc gatttgcttc ccgatctgcc gatttaatca tcaggaagtt tgtcgacttt
     3901 cgctccacaa tatcgctcac ggtgtagctc atagaagacg agcagctaac gggccttcac
     3961 cgggcgtttt ccatggctct cctgcggtac gccgcgagcc aacgacatat gcgaatgaac
     4021 gccaagccag cggccgtccg gctgcctcgc cagaactatt gtggcccggc ccggacggta
     4081 gaaggcggtt ccatcttcat ggaaacccgt gctcgtccag ggcgcgatcg cgactgccat
     4141 cgcgccgtcc ggcgcggcca tgaccatggt gtttgccaag tcgaagcgga actccgccgt
     4201 gcgtggccag acattgtccc attgccgttc cgtccaggcc gatcgactct tcaccagttc
     4261 ctgatacgtg ccgaagatga cgatgtcggg gtgccagaac ggtcgcgccg ccgcgtagtc
     4321 gacctcccgc acgcaaacgg cgaagcgatc gagccactcg cgcacctccg cggaaatctc
     4381 cgaattagcc tctggtaacg gcaagatcat tttacgccta ttttcatccg ctgttattcc
     4441 cagttattga gcgttatgaa agctaagcgc cctgtgttcc cgaacctaca gcgccgcggt
     4501 tatcggcagc gaaaaagtaa aactcgatcc cttgccggcc tcgcttgtca gccagatttt
     4561 gccgccgtgc agctcgacaa actttttagc caaagttaat ccgagtcctg tcccctccct
     4621 cttgtgctcg taatcggagc ctacctggcg aaactcctca aagatcatgg gctggtcttc
     4681 tggtgcgatc ccgatgccac tgtccgtcac cgatatctct accgaaccat tgttctgcct
     4741 tgcagcgatt tcaatccggc ctccctccgg agtgaacttg acagcattgg aaagcagatt
     4801 gagaagtatt tgcttgatct ttctttcgtc gccgatgaaa tccccgagcc gctcgtcaat
     4861 cgcaacatcg agcgtgatgc cgtgcttcgt ggcgcgctcg cggacaaagg tgcgagcatt
     4921 gtcgattgct aacggaagat cg
//
LOCUS       DASUVS010000157        12294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1810837, whole genome shotgun sequence.
ACCESSION   DASUVS010000157 DASUVS010000000
VERSION     DASUVS010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12294
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810837"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VF182_08800"
     CDS             <1..688
                     /locus_tag="VF182_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437217.1"
                     /translation="NGYNPNGLSTYSDSFKKRYFEGQSDRLNELIDEALDIQFHIDRG
                     TYPYPDNAPFVIGRSTASKLFVLDTSILHSTEKPRKLLKDDGTIDDTHIVESVRLPDP
                     TVAQTDETFDAGARLLTVKSFLSTNAIRSTNSIDGIDYCSSNNSTVCNVREITVPVLF
                     TAMGAYYFIRDNEIHFEEAKSGDKDFVVIAGLVHGITPCNACPGGPYNNSQKNFYDYV
                     ASWLNKPGRF"
     gene            complement(1576..1719)
                     /locus_tag="VF182_08805"
     CDS             complement(1576..1719)
                     /locus_tag="VF182_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437218.1"
                     /translation="MFDYRVCADRGKMMKTMTEAEGLELQQLVRERVDKKCLEVSSPV
                     FSL"
     gene            2541..4028
                     /locus_tag="VF182_08810"
     CDS             2541..4028
                     /locus_tag="VF182_08810"
                     /inference="COORDINATES: protein motif:HMM:NF040048.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HEX6437219.1"
                     /translation="MVIFLTAVQWPVELIAAEAYCSGSSTSAVANSGISSRGTRTGLL
                     RVHPSNPRYFTDDSGKAIYLGGHQIFVDLQDNPFGNYTTYNQQNQLDWPWYIQFARAR
                     KLNYLRNWTLMSTGCGVPLQCAVAYPMPYKRVSGFGSANDGGWKFDLNQFDEEFFNRM
                     RSRVIDAGNHGIYVSIMLYDVYAFSNFSAPHSPMWGGNVFNAANNINGVTADTNGDGW
                     GVEFFTAPSSEIRALQKKYVEKVIDAVNDLDNVIFEIGNEVGALQWQYEMIDFVKNYE
                     ASKAKQHLILMSPGGLSGLNVYTSATQSEVIASSADIYAASIVWADYLENPPANNNGK
                     PGILDRDHVRPTHSHVPILAWKAFARGHHYTMYDQPFEAPIEDATWDLARRNVGAIVT
                     YANEVFSDLAKMLPSGDLSSTQYALANPGSEYLIVQPESGAFTVTMQPGTYKYEWFDS
                     DTASTADIGVVSLTAGKNTFTPPFGGSAVLYLKAAPSMESGSPTE"
     gene            4878..5744
                     /locus_tag="VF182_08815"
     CDS             4878..5744
                     /locus_tag="VF182_08815"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437220.1"
                     /translation="MPLISAMAASHAPNILLEPGAEWEDFMDLHYSMAPQGFGSRPTL
                     EQQKKLRRDAEAAFTALRTDLEEAKPDVLIVVANDQFVNFFWNNIPTFFVTLADEVQG
                     QFTRHKFHYRNHKELGREIVRAGMDRGIDFSFGEHIELQHTQNVPLYFLLPEPKIPIL
                     PIYVNTWVDPAPTPKRCYQIGELIREVAVKSSDRVAILATGGLSHFPGSPRIGEIDTR
                     FDHELLEVMRQGQGPSLADYSVDELLKAGDSEFLNWMVVIGAVGNAKATYTSYMPDFV
                     ATGWGFVSWRLC"
     gene            5776..6021
                     /locus_tag="VF182_08820"
     CDS             5776..6021
                     /locus_tag="VF182_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437221.1"
                     /translation="MSCYHLSRLLFDLKMNESCYHRAVADFEEVISDYELTDVEKDAL
                     RSGDPRKLRQLGVHGMLCLYIKRLNPAFRDNIYWAQK"
     gene            6195..6986
                     /locus_tag="VF182_08825"
     CDS             6195..6986
                     /locus_tag="VF182_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6437222.1"
                     /translation="MDLGLKGKVALVAGASQGMGKATALGFAREGAKVSICARGEAAL
                     NQAADMIRKQTGGEVLAMSADMSKAEDIKRFVKSSAEHFGRVDIIVNNAGGPPPGDFL
                     NFTDEDWNNAFNLSFMSTMRMTREAVPHMRKVGAGRVINITSYSVKEPISGLVLSNAI
                     RSGVIGMAKTLSRELGKDNILINNICPGRIDTERAQKLNKARADRLKRPVEEINQEMA
                     AEVPLGRYGTADETADLIVFLGSDRASYITGTTIQIDGGLVRGIQ"
     gene            6987..7310
                     /locus_tag="VF182_08830"
     CDS             6987..7310
                     /locus_tag="VF182_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437223.1"
                     /translation="MVHFVIQWDLPDQKANLTIYANKAKNDWLPATLSHQGVIEIRNY
                     RNPLEITPHVAITIDFENMECWRDYIDSQDYIRIMRELRILGCNNFTANVWAPSKYFA
                     ESMKP"
     gene            complement(7514..8170)
                     /gene="nth"
                     /locus_tag="VF182_08835"
     CDS             complement(7514..8170)
                     /gene="nth"
                     /locus_tag="VF182_08835"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX6437224.1"
                     /translation="MKDREIHAAVRILRREAPKWQTPVVTLIAEASDSPFKVLISCIL
                     SLRTQDSTTAQASRRLFALADLPETMARLPTKKIEQAIFPVGFYRTKAKTILEICRNL
                     NENYRGRVPDEIDELIKFKGVGRKTANLVVTLGYNKPGICVDTHVHRISNRWGYVKTA
                     TPEKTEVALRQKLPKQYWIEYNDLLVSFGQRLCRPISPLCSQCPVAKYCSQIGVTVKR
                     "
     gene            8252..8824
                     /locus_tag="VF182_08840"
     CDS             8252..8824
                     /locus_tag="VF182_08840"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX6437225.1"
                     /translation="MVVHKISQILKVRSPKTIAGNHYKPAAVLIPIQERPDGDYLVLT
                     KRAERMSHHSGQVAFPGGRVDPDDGSAAEAALRESYEEIGIHPDEVRILGQLDQAEAG
                     YGFLVTPFVGVIPASCNFVLNPAETAAVCSVPISALLSPPNFTIDQYLSPRGNPGYHF
                     HVNGWDVWGVTARIIVQFLELVYDFEVKKV"
     gene            complement(8970..9887)
                     /locus_tag="VF182_08845"
     CDS             complement(8970..9887)
                     /locus_tag="VF182_08845"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437226.1"
                     /translation="MAAWPCAAATKIRMAQPVMAMMYAPLYFGVHQGIFAAEGFELEF
                     MTMRTDLAIAALGTGDVDYIVHGGAALRAAAQGFPLKLIFALDHKTPFWLAARPQINN
                     VGQLTGKKIGISFPGDTPQIILKRFLRREGIDPDRDVAYVSGQFSPTALQSLLGGVID
                     AAILAPPFNVIAAEKGLSMLSFLGDSVPDASSSNGIVTSAKKIKSSPEQVRRIVRASL
                     QSLYDFRRNKASATEFLAAHFEIPKNIATKSYANGLDILTRDGEISAEKLRQILSMMQ
                     DIGKKDTVTVSARSLIDFSFLHEAQKELR"
     gene            complement(9956..>12294)
                     /locus_tag="VF182_08850"
     CDS             complement(9956..>12294)
                     /locus_tag="VF182_08850"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX6437227.1"
                     /translation="PAKRPMRRLATVIVSKRAIILGVIIVATLWFAYLSVKIEMYTAF
                     SDLLPADHPYIRVHDRFWKTFGGANVVLLSIEVTEGDIFNVAVLEKIKKLTEMIEQTP
                     GANNYQIFSIARQKVKDVRATAWGIEVQPVMWPNVPQNREEIERLRDIVYANPTIVGR
                     LVSEDGKAALITAAFHEERLDYAELFARIQQAIKQTEDANTRVFAAGEPILYGWIYHH
                     LKSIALIVAFTCLSILAILILYYRNLNGVMIPLLSAGTTFIWGTGFSALMGYNFEPLI
                     LVVPFLIAARTISHSIQFRERFFEELERYGDKEKAAIESAAGLIMPGGVSILTDAIGL
                     TVLLVAPMPILTKLAIAGSFWVLSNLVTVVILDPILCCYFPTPRAASKGGEGHWLDAP
                     LRRVAALCIGPMGRVVVIAGFALVIVWSLYWYQFLTVGDSRPGSPLLWPDAEYNQSVK
                     HINDKFQGTDHLYVIVEGQTESTMKAPPVLEKIEAFQRYMLRSPHVGSSDSLVDLTRQ
                     INTVLHYNDPRWSLLPRTGDEVGGILMVAEHGSEPGDFDRWVNYNFQHGRVTFFLYDH
                     KGDTLREVVDRAAKFIASNPMQGAQFKLASGYAGVLAAANEVIARSDKLTLGLMLLAQ
                     LIFCAIGFRSITAGILFVGVVLMSNTFGMALMAYWNVGLNVNTLPVISLGVGFGEDYG
                     IYIVSRMIEEHRRANRSDLNQALVDGVATAGKAVLYTAFLISASIAFWAFSPLRFQSE
                     MGTQLLIILTMNMLGGLLLLPALIALLKPKFITGYAKP"
BASE COUNT         2937 a         3183 c         3206 g         2968 t
ORIGIN      
        1 gaatggatat aatccgaatg ggctctcgac atactctgat tcatttaaga aaagatattt
       61 tgagggacaa tccgaccgct taaacgaact gatcgatgaa gcgttggata tacagtttca
      121 cattgatcgg ggcacttacc cttatcctga caatgcgcca tttgtcatcg gaagaagcac
      181 ggcctcaaaa ctttttgtgc ttgacacaag cattcttcac agcactgaaa aaccacggaa
      241 gctcctgaaa gacgacggca cgatcgacga cacgcacatc gtagaaagcg tccgcctccc
      301 ggaccccact gtggcccaaa cggacgaaac ctttgatgct ggcgcgcgcc tgctaacggt
      361 caaatcattc ttgagcacaa atgccattcg atccactaac tcgatcgatg gaattgacta
      421 ttgctctagc aataattcaa ccgtatgcaa cgtccgcgaa attacagttc cggtgctgtt
      481 tactgcgatg ggagcgtatt acttcatccg cgataacgag atccattttg aagaagcgaa
      541 gagcggggac aaggattttg tcgtcatcgc ggggctcgtt catggaatca caccatgcaa
      601 cgcttgcccg ggcggacctt ataataattc acaaaaaaac ttttacgact acgtggcaag
      661 ttggttgaat aaacccggca ggttctaatc gggttgtttc ggactatgat ttaacggacc
      721 tgaaacggtt ctggactaag ccactaggct ttcagaacgc gcttctaaca atcgacggat
      781 acttgtaaag ccgctatcgg ccaatccgac cgacgcggaa agatgcttgc tatgtctgac
      841 ttcagagtta aaacgagtca ggggaattcc tttttcttgc gttactttgc tattactttg
      901 ccagaaccat ctaatcgaat gtgttccaat cggcaaattc agtgagagct gagccgcctt
      961 agacgcttgg attcattcag ttgactacca atagtgtcat atagtctcgg taccatcgca
     1021 gaactgaaaa tcctcgtgtc ggcggttcga ttccgtccct ggccacctct aagcctcaag
     1081 tatttcaaac gctgcgcctc gacaaagaag cacaactcat ccactttgaa gttcctgaaa
     1141 ttcgctgctg gtcaaacctt tgtaagttct ggtttgctcg ggtcgtccca atttccctcc
     1201 gctcgacgcg aatatctcaa cgcccctttt ttcgggggta cggctaatta ccgcagtgct
     1261 tctgattctg ccgaggtatt ttacagggac cacgaaaatt ccaggatccc gacgggcata
     1321 gatccgatac catctagatc ccatattagg acgccttcat gggcgatata ttggcctgag
     1381 tgatcaaggt cccaacttct cgctctaatc gggtttccaa gcctatcgct cacggccgcg
     1441 agggtatttg cggttcatcg tttcaatcgt cctgcttggc accgcagact ggacaatgtg
     1501 agaaccaccg ccgaatccga gatcgacatc gccggcagat ggttgttcgc cctcgccgca
     1561 ttaaaagcac tgacgttaca acgagaagac cggcgacgat acttccaaac attttttatc
     1621 cacccgctcc ctgaccagct gctgcagttc gagcccctcg gcttccgtca tcgtcttcat
     1681 catcttgcct cgatccgcgc agactcgata atcgaacatt gaggccattc cgataaacag
     1741 ctccgagcga ctcacattct gacatgaaca tccggaatcg attcgcctgc aatctacagc
     1801 cctgatccta gcatattggc cgaaatttct cgcacgctac gagtcaaatt ctgaatccct
     1861 tgatcgactc cctgtcattg tgctattaac gccgccctcg ccgcgcagcg ataccgttgc
     1921 gctaagcttt tgacagccct gtcgaaggcg ctgcgccggc gaggcccagt actcgatggg
     1981 agaagtcaat ggagacactt acatgaacaa gataatgcgc cagttccggt ctgactacgt
     2041 tgaagatgta ttactcgtta ctttgatgat aatcattgtg gtcgcgcttt atttggctgc
     2101 cgtatatttg ctaattctga gttagctatc gaccttgcag tcgctctctg ccacggtggc
     2161 ctagtctggt attttgacgg gttaagggat tttgaacgtt aactgtgtct gaatgcaccg
     2221 agcgccagca ggagttgaac ttcgctatgg ccgtctttgt acaacagacg cctggcggac
     2281 gcgcaagaaa taattatcaa agtgctgtgt agacaaccct cgcgaggggc agcgataatc
     2341 aaccgcaccc taaaatccgg ccgttattta tctccggtat ccacgaaagg ccacggctga
     2401 cactccgtct cacagcgact attcgcgatg cgctcgccgc cgaaatttca attgtgatga
     2461 atgctttggc accaggaaga ctcactgcgc ggaagctacc gggacggagc atcagacctc
     2521 acctcctgca gctctgttgt gtggtgatat ttctaacggc cgttcaatgg ccggttgaac
     2581 ttatcgccgc tgaggcttac tgctcgggca gtagtacttc tgcggtggcc aacagtggca
     2641 tttcttcccg aggtaccaga actggattgc tgcgcgtgca tccctccaat ccgcggtatt
     2701 tcacagatga ttctggcaaa gcgatttatc tcggcgggca tcagatattc gtcgatttgc
     2761 aggataatcc gtttggcaac tacacgactt acaatcagca aaaccaactg gattggccgt
     2821 ggtatatcca gttcgctaga gcccgaaagc tcaactactt gcgaaattgg accctgatga
     2881 gcaccggttg cggggtgcct ttgcaatgcg cagtggctta cccgatgccg tacaaacggg
     2941 tttcgggatt tggcagtgcg aatgacggtg ggtggaagtt tgatctgaac cagttcgatg
     3001 aggaattttt taaccggatg cgctcccggg ttattgatgc gggtaaccac gggatctatg
     3061 tcagtattat gctttacgat gtctatgcct tctcgaattt ttccgcacct cacagtccga
     3121 tgtggggagg caatgttttc aatgcggcaa acaacattaa tggcgtcacg gccgacacca
     3181 atggcgatgg ttggggagtg gagttcttta ccgcgccgtc atctgagatc cgtgctttgc
     3241 aaaaaaagta tgtggagaaa gttatcgatg ccgtcaatga tctagacaat gtcattttcg
     3301 agatcgggaa cgaggtgggt gcattgcaat ggcagtatga gatgatcgac tttgttaaga
     3361 actatgaggc ttccaaggcc aagcaacacc taattctcat gagccctgga ggtttgagtg
     3421 gtttgaacgt ttatacgagt gccacgcaga gcgaagtcat tgcgagttct gcggacattt
     3481 atgccgcctc aatcgtttgg gccgattatt tggagaatcc gccggccaac aacaacggca
     3541 agcccggtat tctggacagg gatcacgtca ggccgactca cagtcacgtg cccattcttg
     3601 cctggaaagc ctttgccaga gggcatcact acaccatgta tgaccaacct ttcgaggctc
     3661 cgatcgagga tgctacatgg gatctcgcgc gccgcaatgt aggtgcaatc gtgacttatg
     3721 cgaacgaggt attttctgat ctcgctaaaa tgctcccgag tggcgatctc agctcaaccc
     3781 agtatgcact tgccaatccg ggatcagaat acttgatcgt tcagcctgag agcggagctt
     3841 ttacggtgac gatgcagccc ggcacttata aatatgaatg gttcgattcc gacacagctt
     3901 caacggcgga cataggtgtc gttagcctga cggccggcaa gaataccttt acgcctcctt
     3961 tcggtggaag cgccgtgctg tatctcaaag cggcgccatc gatggaatcc ggctcgccca
     4021 ccgagtgatt ttatcttcct caatccgcgc ctaatgctca tcccactctt taaacaaaca
     4081 tgagatggtt cggaatggag ctgtagttta acgcttccgt aacccgagtc ggcaaacgga
     4141 cgagatatga tccgccgatt cggcgatccc acgcttaagt tctctacagt tcaccccagc
     4201 acgttgattt ggttgccgca attgtagtgg cgaacagttt cacctccggc agaatcgtta
     4261 ttctttccag acagttcaac aaggcctctc aattactgca gccaggtgtc tgtcctccct
     4321 tactcgcaag cggcaggatc ccaaagatta ctctcgtgtc tccggcgaga ggttagagtt
     4381 tgtacagtcg ggcggtttgt tgacatcaag tcagttgaaa tggacgcaaa ctgcaagcgg
     4441 ttcatcatgc acatgtttaa gcaagataac gagtacccag ccgcaagatt attgcgatac
     4501 cgtacttcta atcaaaatgg atctaaaaag cttttatgtt ggcgctatag cctgcgtttg
     4561 tttaaaatgt tcgggcattt gtcaactgta gtttgcgaga aaggggctgc ggatggaccg
     4621 actggaaaag ctttttgatt atacgctgag gtatgcactc gaaccagggt tgaactgggt
     4681 tatcgctcat cccatcgtct cagtaatcgt cgtcttcgtg cttgtgtact ggtccgtgcg
     4741 gggttatcga atgctataga ttgcgtggga acatcttgag tttccagtta ctagagattt
     4801 tgctatagct ttcttaagac catgagttta atcgaaacca gagaaagaga cggtgatcag
     4861 gtaaaggggt gcggatcatg ccgctaatct cggcaatggc tgccagtcat gcgccaaata
     4921 ttcttttaga accgggtgcc gaatgggaag acttcatgga tctgcactac agtatggcgc
     4981 cgcaagggtt cgggtcgaga cccacgctag aacaacagaa aaagctcaga cgggatgccg
     5041 aagctgcgtt taccgctttg cgtaccgatc ttgaggaagc caagccggac gttctcatcg
     5101 tcgtggctaa tgatcagttc gtcaatttct tttggaacaa cattccaacc ttctttgtga
     5161 ctctagcgga tgaggtccag ggtcaattta cccggcataa gtttcactat cgcaatcaca
     5221 aagagcttgg acgggaaatc gttcgcgccg gaatggatag ggggatcgat ttctcattcg
     5281 gcgaacatat tgaattgcag catacgcaga acgtgccgct atacttcctt ttgcctgaac
     5341 ccaagatccc aattttaccc atctacgtga acacttgggt cgaccccgcg ccgacaccga
     5401 agcgctgcta tcaaattggt gagctgatcc gagaagtcgc tgtcaaatca tcggatcgcg
     5461 tggcgattct tgctactggc ggcttatccc acttccccgg ctcgccgaga atcggtgaga
     5521 tcgacacccg gttcgatcat gagcttttgg aagtcatgcg ccaggggcag ggaccgagct
     5581 tggccgacta ctctgtcgat gaattattaa aagcgggaga cagcgaattt ctcaactgga
     5641 tggttgtcat tggcgctgtt gggaacgcca aagccactta cacttcttac atgcctgact
     5701 tcgtcgccac cggctggggt tttgtcagtt ggcggctttg ctaggctggt aattaacagc
     5761 ggcgctggag gtgctgtgag ctgctatcat ttaagccgtc tgctcttcga tctcaaaatg
     5821 aacgaatcgt gctatcaccg cgccgtggct gatttcgaag aagtcatcag cgattatgaa
     5881 ctaaccgatg tagaaaagga tgctctccga tccggtgatc cgcgcaagct acggcagttg
     5941 ggcgtgcatg ggatgctttg cctatatatc aaaaggctca atcctgcgtt tcgcgacaat
     6001 atctattggg cgcagaagta gaataacggc gcctcaattt tgtcttttga ctcttgctgt
     6061 ttctctgtga ctttccttcc gatcttcctc gtcacctctc acatcaccta gtttcctggc
     6121 agtcctattc ccattttgac ccgttggaac cggtctggta gtatccgggc acacgtcaaa
     6181 cgagggggaa caagatggat cttggattga aggggaaagt ggcgcttgtt gcaggtgcta
     6241 gccagggaat gggtaaagcg acagccttgg ggttcgctcg tgagggagcg aaggtaagta
     6301 tctgcgccag gggtgaagcg gcgctgaacc aagccgctga catgatccgc aaacaaaccg
     6361 ggggcgaagt gttggcgatg tcggccgata tgagcaaagc ggaagatatc aagagatttg
     6421 taaagagcag cgcagagcat tttggccggg tcgatatcat cgtgaataat gcgggtggcc
     6481 ctcctcccgg cgacttcttg aatttcaccg acgaagactg gaacaacgct tttaatttga
     6541 gtttcatgag caccatgcgc atgactcgcg aggcggtgcc tcatatgcgc aaggttggcg
     6601 caggccgggt catcaatatt acatcttatt ctgtcaaaga accgatctcc ggtttggttc
     6661 tctccaatgc gatccgcagc ggcgtcattg gcatggcaaa aaccctctcg cgcgagttgg
     6721 gcaaggataa tattctgatc aacaatattt gccccggccg catcgatacc gaacgcgccc
     6781 aaaaactcaa caaagcccgc gccgatcgtc ttaaaaggcc ggttgaggaa attaatcaag
     6841 aaatggcggc ggaggtgcct ctgggccgct acggaacggc tgatgaaacc gccgatttga
     6901 ttgttttctt gggatcggat cgagccagct atatcaccgg aaccaccatt cagatcgatg
     6961 gaggtctagt aagggggatt caataaatgg ttcacttcgt gattcaatgg gatcttccgg
     7021 atcaaaaggc caatctgacc atatacgcca acaaagccaa gaacgactgg ctgccggcta
     7081 cgctttcaca tcaaggtgtg atcgaaattc gtaattaccg taacccgctt gagataaccc
     7141 cccacgtcgc gatcacgatc gatttcgaga atatggagtg ctggcgcgac tacattgatt
     7201 cacaggatta cattcgcatc atgcgcgagc tccggatttt ggggtgtaat aacttcaccg
     7261 caaatgtctg ggcgccttca aagtatttcg ccgaatcgat gaagccctaa actatcggga
     7321 tggcgcaatc actgtagcgc gaacgagtgc tactcgatat cgtgtcgatg ggcgaccgga
     7381 tcacatatac ggtgaccaga tgccgggctc cctgagatct gaggatcgcc tggtctgaag
     7441 aaggtttctt ccccgtcatc aaacgaacgt cagcttcggt gtgcgaagaa tcattctaac
     7501 tttgccgtgc tgttcaacgt ttcacagtca caccgatttg actgcaatac ttcgccaccg
     7561 gacattgact gcatagcggt gaaatcggcc ggcataatcg ctgaccgaaa ctcactagaa
     7621 gatcgttgta ctcgatccaa tactgtttgg gcagtttttg gcgtagagct acctccgtct
     7681 tttccggcgt tgccgttttc acgtagcccc agcgatttga aattcgatgc acgtgggtat
     7741 cgacacagat gccgggcttg ttgtacccca gggtgactac caagttggcg gtcttgcggc
     7801 cgacgccttt gaatttgatc agttcgtcga tctcatccgg aactcttccc cgatagtttt
     7861 cattcaagtt cctgcagatc tccaagatag tttttgcttt ggtgcgataa aatcctaccg
     7921 ggaagatcgc ctgctcgatc ttcttagtag gcaatcgcgc catggtctcc ggcaagtctg
     7981 ccaacgcaaa aagccgccgt gaagcctggg cagtcgtgct gtcctgtgtg cgcaggctca
     8041 aaatgcagga aatcagaact ttgaaaggac tatcggatgc ctcggcaatg agggtgacga
     8101 ccggggtttg ccatttaggc gcctcacggc gcagaattcg caccgcggcg tgaatctcgc
     8161 gatctttcac tgagtagttt ttgttttgct gattggctaa attgaaccag gttcatatca
     8221 cagatcactg ttgggtcaac atttctcagc catggttgtt cacaagatct ctcaaattct
     8281 caaagttcgt tcgccgaaaa ccattgcagg aaatcattat aagccggcgg cggtgttgat
     8341 cccaattcag gaaagaccag acggggacta tcttgttttg accaagcgcg ccgagcggat
     8401 gagccatcac agcggccaag tggcattccc tggggggcgt gtcgatcccg acgacggcag
     8461 cgcagcggaa gcggcgctgc gcgagagcta tgaagagatc ggtatccatc ccgatgaagt
     8521 gcgcattctg ggacagctgg atcaagcaga ggcaggctat ggatttcttg tcaccccttt
     8581 tgtcggcgtg ataccagctt cttgcaactt cgtactgaat cccgcggaaa ccgcagcggt
     8641 gtgctcggtg ccgatctcag ctttactatc gccacccaac tttaccattg accaatatct
     8701 ttcgccccgg ggcaatccgg gctatcactt tcatgtgaac ggctgggatg tctggggcgt
     8761 caccgccagg atcatcgtgc agttcctgga acttgtttat gacttcgaag tcaagaaagt
     8821 ttgattctgt cgctgtcttg actcatccgc caacattccg gtaaatccca gagtcatgcc
     8881 tcagcccggt ctatcgtcgc taattatcgt cacggctttc atctaagtgt aatcaagcat
     8941 gatgatcagg gaggactgac gctcgcctgc tatctgagct ccttctgtgc ttcatggagg
     9001 aaagagaaat cgatcagtga acgcgcactt accgttacgg tatctttttt cccgatatcc
     9061 tgcatcatcg atagaatctg gcgcaatttt tcggcgctga tctcgccgtc ccgggtcaaa
     9121 atatccagtc cattagcata cgatttggtc gcaatgttct tcgggatttc gaaatgggct
     9181 gcgagaaatt cagtagccga tgctttgttg cggcgaaagt catagagcga ttgtaggctc
     9241 gcgcgcacga tgcgcctcac ctgttccggg ctcgatttaa tctttttcgc gctggtgacg
     9301 ataccgttgg aagagctggc gtcaggcacg gagtcgccaa gaaacgaaag catgctcaag
     9361 cccttctcgg cagcgattac gttgaacggc ggcgccagta tagccgcgtc tatcaccccg
     9421 cccaagagac tctgcagcgc cgtcggcgag aattgacctg agacgtaggc aacatcgcga
     9481 tccggatcga tcccctcgcg gcgtagaaag cgtttgagaa tgatttgcgg cgtatcgccc
     9541 ggaaagctga tgccgatttt ttttcccgta agctggccga cattgttgat ttgcggccgc
     9601 gcggccagcc aaaacggtgt cttgtggtcg agggcgaaaa taagcttaag cgggaagccc
     9661 tgggcggcgg cgcgcaacgc agcgccgcca tggacgatat agtcgacatc ccccgtgccc
     9721 aatgcggcaa tcgccagatc cgtccgcatg gtcataaatt ccaactcaaa tccctcggcg
     9781 gcaaagatcc cttggtggac gccgaaataa agcggcgcgt acatcattgc catcaccggt
     9841 tgagccatgc gaatcttagt agcagccgcg cacggccatg ctgccatcag cacaccgatg
     9901 aggatgacgc tcagaagatt tcgtttaaaa gcgtctcgaa cggagacaga agatctcatg
     9961 gctttgcata tccagtgatg aatttgggct ttagtagcgc aatcagcgcc ggcaacagca
    10021 gcaaaccgcc tagcatgttc atcgtcagta taatcaggag ctgagtgccc atctcggatt
    10081 ggaagcgcaa tggcgaaaaa gcccagaagg cgatgcttgc cgagatcaga aacgcggtgt
    10141 acagaaccgc tttgccggcg gttgccaccc cgtcgacgag cgcctggttt aaatcgcttc
    10201 ggttcgcacg ccggtgctct tcgatcatcc gcgaaacgat ataaatgcca tagtcctcgc
    10261 cgaagccgac gcccagactg attaccggca gtgtattgac gttaagtccg acattccagt
    10321 acgccattag cgccatgccg aaggtatttg acatcaaaac gacgccaaca aacagtatgc
    10381 cggccgtgat cgagcgaaaa ccgatagcgc aaaaaatgag ttgcgcaagc agcatcaatc
    10441 ccaaagtgag cttgtccgag cgggcaataa cttcattggc cgcggcaagc acgccggcat
    10501 agccgctggc aagtttgaac tgagcacctt gcatgggatt gctggcgata aacttcgccg
    10561 cccggtcgac gacttcccgt aaagtatcgc ccttgtgatc gtagagaaaa aaagtcacac
    10621 ggccgtgctg aaaattgtag ttgacccacc gatcgaaatc gcccggctcg ctgccgtgct
    10681 cggcgaccat caggattccg ccgacctcgt cgccggtgcg cggcagcagg ctccagcgcg
    10741 gatcattgta atgtaaaacc gtattgatct ggcgagttag gtcaaccaaa gagtcgctgg
    10801 agccgacatg gggtgagcgt agcatgtaac gttgaaaagc ttcgatcttt tccaacactg
    10861 gcggcgcctt catcgtgctc tcggtttgtc cttcgacaat aacgtaaagg tgatcggttc
    10921 cctggaattt atcgttgata tgtttgacgc tctggttgta ttcagcgtcg ggccaaagca
    10981 gcggagaccc gggccgcgaa tcgccaaccg tgagaaactg ataccaataa aggctccaaa
    11041 cgatcaccag ggcaaatccg gcgatgacca cgactcggcc cataggaccg atgcaaagag
    11101 cagcgacgcg gcggagcggc gcgtcgagcc aatgaccttc accgcctttg gacgccgctc
    11161 gcggggtggg aaaatagcag caaaggatcg gatcgaggat caccacggtt acaagattgc
    11221 tgaggaccca gaagcttccg gcgatcgcca gcttcgtgag tatcggcatg ggcgcgacca
    11281 ggagaacggt taagccgatg gcgtccgtca agattgaaac accaccgggc atgatcaagc
    11341 ccgccgccga ctcgatggcg gctttctctt tatcgccgta gcgttcgagc tcttcgaaga
    11401 agcgctcgcg aaattgaatg gaatgactga ttgtccgagc ggcgatcaaa aatggcacga
    11461 ccagaatcag cggctcgaag ttgtatccca tgagagcgct aaagccggtt ccccaaatga
    11521 aagttgtccc ggcggacagc agcggaatca tgacgccgtt gagattgcgg tagtagagaa
    11581 tcagaatcgc caggatgctc aaacacgtaa acgccacgat cagggcgatg gatttgagat
    11641 gatgataaat ccagccgtag agaatcggtt cgccggcggc gaaaactcgg gtgttggcat
    11701 cttccgtttg cttgattgct tgttgaatgc gggcgaagag ttcggcatag tcgagccgct
    11761 cttcatgaaa tgccgcagtg ataagcgcgg ccttgccatc ctcggaaacg agccgcccga
    11821 caatggtcgg attagcataa acaatatcgc gcagccgctc gatctcctcg cgattctgcg
    11881 gcacgttggg ccacatcacg ggttgcactt cgatgcccca tgcggtggcg cgcacgtctt
    11941 tgaccttctg tcgcgcgatg gaaaatatct gataattgtt ggcgccggga gtttgctcga
    12001 tcatctcggt gagttttttt attttttcca gcaccgcaac gttgaagata tcgccctcgg
    12061 tgacctctat agatagaagc acgacattgg cgccgccgaa ggtcttccag aaccggtcat
    12121 ggacacgaat atagggatgg tcggctggca gaagatccga aaacgcggtg tacatttcga
    12181 ttttgactga aagataagca aaccacagag ttgcaacgat aatgacgcct aaaatgatgg
    12241 cgcgcttact aacaatgacg gtggcaagcc gtctcatcgg gcgctttgct ggtt
//
LOCUS       DASUVS010000158        14264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_718786, whole genome shotgun sequence.
ACCESSION   DASUVS010000158 DASUVS010000000
VERSION     DASUVS010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14264
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718786"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="VF182_08855"
     CDS             <1..1459
                     /locus_tag="VF182_08855"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6437228.1"
                     /translation="QGRRLAAAPINSPSAFLEDEQTCHRGYVVELDHTHLGRHRFPGD
                     PYKFSESPWHIGRGAPLMGEHQSMIGSDWTQPSPALASLKSPESNSRAFAGIRVISFP
                     TGVVGPALGCLLAEHGAEVISIEAGRTIRSPQRGQQFQIASDLESNRGRKRIAINMKH
                     REGVDLAKRLIAQSDVVAENFSARVMASWSLDYPRMREVRSDIIMASLQAFGQTGPRR
                     DYVSFGPILMAYSGMAYLWRDPKSERPGAACQTAFPDYIAPSYGALAILAALHYRART
                     GKGQYIDLSQAETAASLIAPAHLEWLLTGQEPQPSGNFSASAAPHGCYRCKGEDRWCV
                     ISVQTEEEWARFCDVVGHREWLADGRFIDLSSRVAHGLELDALVESWTARHTPHQVLM
                     ILQRNGIAAGVVQTAEDLYRDPHLRERGSAREVFHQPLGWVTRAGPSVRSSDAKFAQK
                     EQAHFAGDDNEAVFGELLGMSSRQIADLRQRDVLR"
     gene            complement(1513..1731)
                     /locus_tag="VF182_08860"
     CDS             complement(1513..1731)
                     /locus_tag="VF182_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437229.1"
                     /translation="MKILLLALAILVNACASPPPPTTQTRDKTTTTASPSSASRDTTD
                     CERQAALAGAGERAKAFENCMRAKSRTP"
     gene            2095..3156
                     /locus_tag="VF182_08865"
     CDS             2095..3156
                     /locus_tag="VF182_08865"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6437230.1"
                     /translation="MGAIDADAHVIETVQTWSYLENDERHFTPQLMTQTFGEKLLSNE
                     GIAVPEFWVIENRAFGKDRNVGTDTSKESREMLSVQARLAHMDELGVETQVLYPTLFL
                     RPVLQDAERERALCKSYNRWMGELWRQGQGRLRWVAKPPLRSLEKTPAAVRLELQWGK
                     ENGACGIFMRGLECERALGAPYFYPLWEMAAELDLPVCIHSANGSFIHHDFFAEDTSF
                     TKFKLTVVGAFHTLLEKQIPKKFPQVRWGFVEVSAQWVPYVLNDLADRYRRQGRSFPA
                     DPLASNNLWVACENTDDLPYVLHHAGEDCLMIGTDYGHHDPSTELNAIHLLRKDKRIA
                     PEVASKILETNPKKFYGFN"
     gene            3194..3439
                     /locus_tag="VF182_08870"
     CDS             3194..3439
                     /locus_tag="VF182_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437231.1"
                     /translation="MPHIPYADPASISDPEILGYLELARKQGTPRPESQAIRANNPSV
                     IRAFSQAWELTYRQGVCDHALKELCRVYVSKSIDCEY"
     gene            3614..3775
                     /locus_tag="VF182_08875"
     CDS             3614..3775
                     /locus_tag="VF182_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437232.1"
                     /translation="MHQHFTTEELVELGFFVALTLGQQRWIKTLGIAHGEVLGDTTTG
                     LAPTPKVKS"
     gene            complement(3850..4230)
                     /locus_tag="VF182_08880"
     CDS             complement(3850..4230)
                     /locus_tag="VF182_08880"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6437233.1"
                     /translation="MKPKFIKHDPPRRYSKASVYGDFIFLAGEDSKDPKTQKVRGTTA
                     AEQAEYLFQNMKATLESLGSGLEDVIKTTIYLTDPRHADGYREGRKKYLPQAPPSTLI
                     MGVQLAEPEMLIEIEAVAVISEKK"
     gene            complement(4244..5323)
                     /locus_tag="VF182_08885"
     CDS             complement(4244..5323)
                     /locus_tag="VF182_08885"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437234.1"
                     /translation="MSRRAIPAQGLALFNFLIFSLVKRNMQFCHNRDFRPKLALFAAI
                     VILVLPALAAAQSPLKVRAAYGAMSAAMAPLWYARDTKLFDRFGVHVELLYIQGQPIA
                     LPALVTKEIDVLLTSGGSAVPFIMNGLPLTMVANLNNIQPYRLLVAPEIKNASDLKGK
                     KVAVSRIGATSHQLAVMSLEKLGLKSNDVTYLQVGGQPERLAALKVRAVDATLINPPA
                     NLTARKLGFNEIFDLKELGENFLFQVLVTRKTDSDMKVEWLQRFIKMTMEAVRLMKAD
                     QQTTIKIIGKYLKETDREALEESFKVHALSMVDYPLVSRSAVEDAYNDALKALKGTAP
                     RNTNIDSMYDNSLLESIKGGAQSGK"
     gene            complement(5368..7011)
                     /locus_tag="VF182_08890"
     CDS             complement(5368..7011)
                     /locus_tag="VF182_08890"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6437235.1"
                     /translation="MKSRYSPHPDLILHSGKIRTFAGNGSICEALACAGGRIVATGSS
                     DEIRRLAGPDTESFDLKGRTVIPGLTDTHVHLSEKGTAEKELVDCRDFYVDVNSVADI
                     LQRLANAAASVPKGSWIVAHGSPMQDFRIEDKRFPDRHDLDRVVPDHPVSISFGAHVT
                     IANTPALAAAKITRDTADPAGGHIRHDPQTGEPTGELHERAQLIVKKVAPEFNYLQLK
                     DGIVFALDQCLQRGVTTVHDIVRYAEPVRAYQELYKEGRMHARVSILPRVIESMIESK
                     SLIELGLITGFGNDWLRIGGVKMSIDGGITGRNACFHEPYENDEHNHGIIRIQQDELN
                     HTVQVCHDAGLRCCVHAIGDKAFDMALDAYENAIEHSPRKDHRHRIEHMGNWLATPKL
                     MQRMVRSGIVAIPNIAFAYYIGDAILDCVGEKRLTKAFPFRTLLKNGIIIAGGSDSPG
                     YWPVDPLRDIATAASRKMRWGDVWVPEERISVSEAFAMHTTTASWVGFEENDKGTLEV
                     GKLADIAVLAEDPFEIQPERIKDLKVEMTLVGGEVKYQA"
     gene            complement(7167..8246)
                     /locus_tag="VF182_08895"
     CDS             complement(7167..8246)
                     /locus_tag="VF182_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437236.1"
                     /translation="MSLKRANRRAGGPFVAAICLLATVNVFAASAPMSDAQIALYQGG
                     DREKILVDGAKREGQLTLYNSHTWFRTFVRDFEKKYPFIKVSEWRSDSKNLLSRAMAE
                     FGSTRSLVDVIETTAEAMGVMKRNGMFQEYFTPEARYYPADVQVKGATGLYYLGDRET
                     YNSLGFNTSLIAPNEAPKALNDLLDPKWKGKMAIAGTSTGVRWVGSALDTQGREFLNK
                     MAAQELTVQNMSGAALAGLVASGEVPLSPTIFDADVTVAKQKNAPIQWRALDPVVTTV
                     GYSGLSSKASHPHAALLFLDYLHGREGQQLMMKGGLWSPREDTGSLDQKFEKLYLDSK
                     YSLDELEKKLVEWENLMRQLFIRRK"
     gene            complement(8697..9638)
                     /locus_tag="VF182_08900"
     CDS             complement(8697..9638)
                     /locus_tag="VF182_08900"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6437237.1"
                     /translation="MDSTILITGANGFIGSHTTLLMESLGHKVVPIDVLPRSTDLSLL
                     AIQTPSRIMNVTDEALFRELCRLEQPTHIFHAAHPPRDDSPEVLNFCYRAMTNILEAA
                     RELKLSSVVYSSSGALYGQLRKKDHAPIKEDDPVAIYPTYFYRSGKIMSEWMGDFYKQ
                     KFGVPFVALRYSSVYGPGLARGIPLELKKGVLGQPCRPYLTRLPDDLIYIDDVTDAVR
                     RALFTEEPLSRAYNIAVDQVYGNQDLEDSIRKALPEISFEIGKHPHASTVGAHRDRDP
                     LDITRAKEELDWRPIIDLENGIARVAAWLKSYANRLK"
     gene            complement(9811..10881)
                     /locus_tag="VF182_08905"
     CDS             complement(9811..10881)
                     /locus_tag="VF182_08905"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6437238.1"
                     /translation="MLRRLKLAVLLLTIVAHAPLLQGAQSRPASVGELALYQGADREK
                     ILVEGAKKEGQLTFYTSNTWVAGPVSQEFEKKYPFIKANVWRSDSKALLKRLTEEHAA
                     GKYLADVVETSPEYVTLLVRASLLQDHFSPELSAYDDEAKVKGKTGVLAWTNREIYIS
                     LGYNTNLVPAAEAPKTLKDYLDPKWKGKMAIAGTTTGTQWIGALWDAMGREFLEKLSA
                     QEINVQNMSGAALSGLVASGEVPLSPTIFNSNIFTHKQKNAPVEWRPIDPVIAGVGSS
                     GLIVNAPHPHAALLFLDYLHSKAGQQVAMKGGLSSARNDIGSLEQKFKKIYMESKYTP
                     EEMEKKFGEWEGLMRKLFIRQR"
     gene            complement(10909..12090)
                     /locus_tag="VF182_08910"
     CDS             complement(10909..12090)
                     /locus_tag="VF182_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6437239.1"
                     /translation="MAAKSAAIEKFLKKLDSADLLIKFNWDEWLTIDPPYSDTKISTQ
                     DVTARYHRNGYDWDMHGTLFSPEKELDSNRAFVFFHGGAGSEKIMDLTPDGRPGLARV
                     VASQGFNVLALTYPGHYPKGGVWKQPIAERMPIYLVDRELPTEEILDRNLKCTFNVIL
                     QGAGLLTDQRFAGREIIGFGHSTGGPMAAHLTRFSKKTRVIGLVGFGSGGPDGWRLEW
                     RNQTGAEKYSEKPVDHVSRRSPDSFKASGYVDPEDLTPWGGADDYIRLISPLRSQMKT
                     SLCDNQHNAAVGILDEYVKRTGLPREEYFDHLQEPDPAWLKTIRVLLTVGENDKGHWV
                     KGERLEDKREMFMGAKYAASTRGAHVVLIPRYGHVGYSELYNEKIAYLWLWASKAGYF
                     D"
     gene            complement(12098..12928)
                     /locus_tag="VF182_08915"
     CDS             complement(12098..12928)
                     /locus_tag="VF182_08915"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6437240.1"
                     /translation="MRPAPIKLPNDAKIAVSFFVAFEAFEKYSQYREGGDKPDLASLA
                     YGEYGGKAGIWRIMDVLARNQVRGTIDTNGRVAEDFPDALKELHAGGHEIVGHGWVND
                     TSLISLEPAREKELIKRTLDSLTSVTGKRPIGWVSPGHRINEFTFAHFVDEGILWTGD
                     LPGPDIPQHRVVNGKPLVIMPRLDYANDLSLIFRAKNPARVYFESFKTAFDLLYAEGR
                     AGSPKLIDALVHAHIGGRPNIIGVFEECIRYAKGFKDVWFCTKGETATWYRDNYVGKQ
                     "
     gene            complement(13179..>14264)
                     /locus_tag="VF182_08920"
     CDS             complement(13179..>14264)
                     /locus_tag="VF182_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX6437241.1"
                     /translation="TGKKWALTREAPEQPRYLVMNGGEDEPGSKKDRVLMENVPHLVI
                     EGVILSAYAIGAKKAYLYINAEYETALKSISDALAEAKSEGYWGENVLGSNFSLDIEI
                     VPAPHNYVAGEDTAVLEVIEGKKAWPRQKPPFPVTVGLFGKPTSVNNVETLANVAPIV
                     LHGAEWYRKFGTADSPGTMLFALDEDVNRPGVYELPFGTPLRYLIEECGGGLKNGKKI
                     KAVMPASPSCAFLPPEKIDTPLDHNSMREAGAGIGCGVIRLVAEGECIVEEVLRIAEF
                     FAAESCGQCPACRMETSTLAMMMKKVQQGQGGQPILEQFGKILAFNKGKGFCNLINMP
                     GPPIESAIKLFPGDFEAHLTTGKCPVN"
BASE COUNT         3128 a         3924 c         3831 g         3381 t
ORIGIN      
        1 tcaagggcgg cggttggccg ccgccccgat taattcgccc agcgcctttc tcgaagatga
       61 acagacctgc catcgcggtt atgtcgtgga actcgatcac actcatttag gccgtcaccg
      121 ttttcccggg gacccctata aattctctga aagcccgtgg cacatcggcc ggggcgcgcc
      181 gctgatgggc gaacatcaat caatgatagg gagcgactgg acgcagccgt cgccggcgct
      241 ggcgtcattg aaatcgccgg agagcaatag ccgagctttt gcgggcatca gagtcatcag
      301 ttttccaacc ggcgttgtcg gaccggccct gggctgcctg ttggctgaac atggcgcaga
      361 ggtgatctcc attgaagccg gtcggaccat ccgcagcccg cagcgcggcc aacaatttca
      421 gattgcctcc gatctcgaaa gtaaccgcgg ccgcaaacgg attgccatca acatgaagca
      481 cagggaggga gtggatctcg ccaagcgcct cattgctcaa tcggacgttg tcgcggaaaa
      541 tttcagcgcc cgggtgatgg cgagttggtc actggactac ccgcgcatgc gcgaagttcg
      601 gagcgacatt atcatggcga gccttcaagc ctttggtcag acggggccac ggcgggatta
      661 tgtcagcttc ggaccgattc tcatggctta ttccggcatg gcttatctct ggcgcgatcc
      721 caaaagcgag cggcccggcg ccgcttgtca gaccgccttt ccggattaca tcgcgccctc
      781 ctacggcgcg ctggcgattt tagccgcgct tcactatcgc gcgcgtaccg gtaaaggcca
      841 gtacatcgat ctgtcacagg cggagacggc tgcatcgctc atcgctccgg cacatctgga
      901 atggctgcta actgggcagg agcctcaacc gagtggaaat ttcagcgcat cggcggcgcc
      961 acacggctgc tacagatgca agggcgagga ccgctggtgc gtcatctccg tgcagacgga
     1021 agaagaatgg gcgcgtttct gcgatgtggt cggccatcgc gagtggcttg ccgatggtcg
     1081 attcattgat ctttcatccc gggttgccca cggtttggag ctcgatgctc tcgtcgaatc
     1141 gtggacggcg cggcacacgc cgcatcaggt gctaatgatc ttgcagcgta acggcatagc
     1201 tgccggtgtg gtccagaccg cggaagatct ctatcgcgat ccgcacctgc gcgaacgcgg
     1261 ttccgcccgg gaggtctttc atcagccgct tggttgggtg acgcgcgcag ggccgtcggt
     1321 gcgttcgagc gatgcgaaat ttgcgcagaa agagcaagcg cactttgccg gcgacgacaa
     1381 cgaggcggta tttggcgaac tattgggcat gtcgagccgg cagattgccg atctcagaca
     1441 acgagacgtg ttgcgctagt ccggctgcgg agggcgcaat ctccgcagtt tgagatcgtg
     1501 atccgtttaa cgctatggcg tccggctttt cgctctcatg cagttttcaa aagctttggc
     1561 cctctcaccc gcgccggcta gcgcagcctg gcgctcgcaa tccgtggtat cacgactcgc
     1621 gctgcttgga cttgccgtcg tagtggtttt atctctcgtt tgagtcgtag gcggcggcgg
     1681 gctggcgcac gcgttgacca aaatcgccaa agcaagcagt agaatcttca tgcgtttcct
     1741 tcctgcggaa agaagtgtca gagaagcttt tttccgcaat cctcccttcg ttcgcagaac
     1801 tctagtttct acccaatatc ggcacagcgg agaaatcaca agcggcatga agtccgatgg
     1861 aagcggcagg aattcggcgg taaatagcat tcaactgtgg gacatcgtct gagcctcggt
     1921 gattcaagct atagaattga gcggctctat tgaattgccc gcttcaaagc gatcatgtta
     1981 agacatcccg gtgcggtaga gtgttgttga tgatccactg agaagccggc gctccgaacg
     2041 atggcctaag gacctcgaca cggagagtcg tacgttagtt caaggaggga accgatgggc
     2101 gcgattgatg ctgatgctca tgtgatcgag acggtgcaga catggagcta tctggagaat
     2161 gatgaaagac atttcacccc gcaactcatg actcagacct ttggcgagaa acttctcagt
     2221 aatgaaggaa tcgccgtccc cgagttttgg gttatcgaga accgcgcctt tggcaaggac
     2281 cgcaacgtcg gcacggatac gtcaaaggaa tcgcgtgaaa tgctcagcgt tcaggcgcgg
     2341 ctcgcgcata tggatgagct cggcgttgaa acgcaggtgc tttatccgac actttttctc
     2401 cggccggttc tgcaggatgc cgaacgcgaa cgtgctctat gcaaatctta caatcgctgg
     2461 atgggcgagc tctggcggca gggccagggt cgattgcgtt gggtggccaa gccccctctg
     2521 cgttcactcg aaaaaacgcc tgccgcggta agactggaac tccaatgggg caaggagaac
     2581 ggcgcctgcg gcatcttcat gcgcggcttg gaatgcgagc gggcgttggg cgctccctat
     2641 ttctacccgc tctgggaaat ggcggcggaa ctcgatcttc ccgtctgcat tcattcggcc
     2701 aacggcagtt ttatccatca tgacttcttc gccgaggaca ctagctttac taaattcaaa
     2761 ttgactgtcg tcggcgcttt tcataccttg cttgagaagc agattccgaa aaagtttccg
     2821 caggttcgct ggggctttgt cgaggtcagc gcccaatggg tgccctatgt tctaaatgat
     2881 ctggccgatc gctaccggcg gcagggcagg tcgtttccag ccgatccgct cgcgtccaac
     2941 aacctgtggg tggcctgcga aaataccgat gatctgccgt atgtcttgca ccatgcgggc
     3001 gaagattgtc tgatgatcgg caccgactac ggtcaccatg atccctccac cgagctcaac
     3061 gccattcatc tgctgcgcaa agataaacga atcgcgcctg aggtggccag caaaattctc
     3121 gaaacgaatc cgaaaaaatt ctacggcttc aattgatgca gcctagcgcg actgactgaa
     3181 ggaggtagag acaatgccgc acattcccta tgccgatccg gcatctatca gcgacccgga
     3241 aattttgggt tatctggaat tggcacggaa gcaaggtacg ccccggccgg aaagtcaagc
     3301 gatccgggcc aataatccaa gcgtcattcg agccttctcg caggcgtggg aactgactta
     3361 tcgccagggc gtttgcgatc acgctctcaa ggagctctgc cgtgtttatg tttcaaaatc
     3421 catcgactgt gaatattgag gagtccaacg atccgtccag gctggacgga agggtctgac
     3481 ggaggacaag atcgacgacc tgctgaattt tgccgattct gaaaagtttt cagaacgcga
     3541 aaaggtcgcg ctgacctata ccagcgcgat tgtctggaac tcagaaattg ctgacgaggc
     3601 actgtggcag aagttgcatc agcactttac caccgaagag ttggttgagc tcggcttttt
     3661 tgtcgcgctt accttggggc agcagcgctg gatcaaaacc cttggcattg ctcacggaga
     3721 ggttttaggc gatacgacga ccgggctcgc tccaactccg aaagtaaaaa gctgacgcaa
     3781 gcagcgtcat tgaaccttaa agagaaagag tgcaaccaca ccatctagca gcgacctctg
     3841 acatggccgt cacttttttt cagaaattac ggctaccgct tcgatttcga tcagcatctc
     3901 cggttcggcc aattgaacac ccatgataag ggtgctcggc ggcgcctgcg gcagatattt
     3961 cttacgccct tcgcggtaac catccgcgtg gcgagggtcg gtgagataga tcgtggtctt
     4021 gatgacatcc tcaagtccac ttcctaaact ctccaaggtt gctttcatat tctggaacaa
     4081 gtactccgct tgctctgcgg ctgttgtccc cctcaccttt tgagtttttg ggtctttgga
     4141 atcttcgccc gcgagaaaaa tgaaatcgcc gtaaaccgac gctttggaat aacgccgagg
     4201 cggatcgtgt ttgatgaatt tgggcttcat agttacctgt ctactacttt ccagattgcg
     4261 ccccaccttt gatactctct aacagactgt tgtcgtacat actgtctatg ttggtgttcc
     4321 ttggagcagt tcctttcagc gccttgagcg cgtcattgta ggcatcctcc actgcgctac
     4381 ggctcaccag cggatagtcg accatgctca gcgcatgaac tttgaagctt tcttcaaggg
     4441 cttcgcggtc tgtctcctta agatacttgc cgattatttt gatagtagtc tgttgatcag
     4501 ccttcatcag ccttactgct tccatggtca tctttatgaa acgttgaagc cattcgacct
     4561 tcatatccga atcggtctta cgagtgacga gcacttgaaa gaggaagttc tctcccaatt
     4621 cttttagatc gaagatttca ttgaatccca gcttacgagc cgtcagattg gcgggcgggt
     4681 taatcaatgt cgcatcgacc gcgcgcactt tgagagccgc aagcctttcc ggctggccac
     4741 ccacttgtaa gtaagtgacg tcattggatt taagtccgag cttttcaaga ctcatgacag
     4801 cgagttgatg actcgtggcg ccgatccggc ttacggccac tttcttgccc ttcaagtcac
     4861 ttgcattttt gatttccgga gcaaccagga ggcgatacgg ttgtatgttg ttaaggttgg
     4921 caaccatggt caacggcaag ccattcataa taaaaggtac agcgctccca ccgctggtca
     4981 acaacacatc gatctccttt gtcacaagcg ctggcagggc tatcggctgg ccctgtatat
     5041 atagcagctc tacatgtact ccaaacctgt cgaacagctt ggtgtctcgg gcgtaccaca
     5101 acggcgccat agctgcggac atcgctccat aagctgctct tactttgagt ggtgattgtg
     5161 ccgccgccaa ggctggcaat acaagaataa caatggcggc aaacaatgct aatttcgggc
     5221 ggaaatcacg gttgtgacag aactgcatat tccgcttaac caaagagaag atcaggaaat
     5281 tgaacaaagc caatccctga gcagggatcg cgcggcgact cacatgcgca tcactgattg
     5341 cgagtgcggc atccgtggtc ctttatccta ggcctgatac ttcacctctc cgccgaccag
     5401 tgtcatctcg accttgagat ccttgatgcg ctccggttga atctcaaatg gatcttcggc
     5461 caggacagcg atgtcggcaa gcttgccaac ctccaatgtg cctttgtcgt tctcctcgaa
     5521 acctacccat gacgccgtcg tggtatgcat cgcaaaggct tcgctgacac tgattctttc
     5581 ctcgggcacc cacacatcac cccagcgcat ctttcgtgat gccgcagtgg caatatcccg
     5641 tagaggatcg accggccagt agcccggcga atcggagccg cccgcgatga tgatgccgtt
     5701 cttcaagagt gttcgaaatg gaaaggcttt ggtcagccgc ttctcgccga cgcaatcgag
     5761 gatcgcgtcg ccgatgtaat aggcaaaagc aatatttgga atggcgacga tcccggatcg
     5821 taccattcgc tgcatgagtt tgggcgtggc cagccagttg cccatgtgtt cgatacgatg
     5881 gcgatggtct ttgcgcggcg agtgctcgat agcattctca taggcgtcca gcgccatgtc
     5941 gaaagctttg tcgccaatgg cgtgaacgca gcagcgcagg ccggcgtcgt gacatacctg
     6001 cacagtatga ttcagctcgt cctgctgaat cctgataatg ccatggttgt gctcgtcgtt
     6061 ttcataaggc tcgtgaaaac aggcgttgcg tccggtgatt ccgccgtcga tgctcatctt
     6121 gacgccgccg atgcgcaacc agtcgttgcc gaagccggtg atcagaccga gctcgatcaa
     6181 gctctttgat tcgatcatag actcgatcac ccgcggcaaa atactcaccc gggcatgcat
     6241 gcggccttct ttataaagtt cctgataggc gcgcaccggc tccgcgtagc ggacgatgtc
     6301 gtgaaccgtg gtcacgccac gttggagaca ctgatccaat gcgaagacga ttccatcttt
     6361 caattgtaaa taattaaatt ccggggcgac ttttttgacg atcaattgcg cacgttcgtg
     6421 caattcgccg gtgggctctc cggtttgcgg atcatgcctg atgtggccgc cggcaggatc
     6481 ggcggtatcg cgtgtaatct ttgccgcagc cagagccggc gtattggcaa tggtgacatg
     6541 cgcgccgaag gaaatggaaa ccggatgatc gggcacgaca cggtcaagat catgccggtc
     6601 gggaaagcgc ttgtcttcga tgcggaaatc ttgcatcgga ctgccatggg cgacaatcca
     6661 cgagcccttc ggcacgctag cggcggcatt ggcaagtcgc tgcaaaatat ccgcgacgga
     6721 gttcacatcg acataaaaat cgcggcaatc gaccaactcc ttttcggctg tgcccttctc
     6781 tgaaagatga acgtgcgtat ccgtcaatcc agggatgaca gttctgccct tgagatcgaa
     6841 agattccgtg tcgggcccgg ccagccgtcg aatctcgtca gaagatcccg tggcaacaat
     6901 acgaccgccg gcacaagcca gcgcctcgca gatcgaacca ttgccggcaa aggtgcgaat
     6961 cttgccgctg tgcagaatca aatcaggatg cggtgaatac cgtgatttca tcgagagcta
     7021 ttcctccttg cagattgcga caccacctag ccataagttg cttcgatatc ggagtcaata
     7081 gcgtcaagca cgagatccgg ggcgatcttc tctgttactg ataatatgga cgcacccccg
     7141 gatattcggt agctcttatt cgacgttcac tttcttcgaa taaaaagctg gcgcatcaaa
     7201 ttttcccact ctacgagctt cttttcgagc tcatcgagtg agtatttgct gtcaaggtag
     7261 agtttctcaa atttctgatc caaagatcca gtgtcctctc gaggcgacca aagcccgcct
     7321 ttcatcatta actgttgtcc ctccctgcca tgcaggtagt caagaaagag caaagccgca
     7381 tgcggatgag aggctttaga tgataaaccg gaatagccga cggtggtgac gaccggatcc
     7441 agagcacgcc attggattgg cgcattcttt tgcttggcaa cggtcacatc ggcatcgaaa
     7501 atcgttggcg ataacggaac ttctccggat gctaccaaac ccgccaaagc cgccccggac
     7561 atattttgca ccgttaactc ctgtgcggcc attttgttca gaaactcacg tccctgtgtg
     7621 tccagtgcgc taccaaccca cctaacgccg gtggatgttc cggcaatggc cattttcccc
     7681 ttccactttg gatcgagcag atcgttaagc gccttcggcg cctcgttcgg agcaatcaga
     7741 ctagtgttga accctaagct attgtaagtt tcccgatcgc caagatagta aagtccggtc
     7801 gctcctttga cttggacatc ggctggatag taacgcgcct ccggcgtaaa atactcctga
     7861 aacatgccgt tccgtttcat cacccccatg gcttcagcgg tcgtttcaat gacatccacc
     7921 aaagaacgag tagaaccgaa ctccgccatg gctcggctga gcaggttttt cgagtcgctg
     7981 cgccactctg acaccttgat gaaaggatat tttttttcga aatctctgac gaaagtgcga
     8041 aaccacgtgt gtgaattata aagggtgagc tgaccttcac gcttggctcc gtcaacgaga
     8101 attttttccc tatccccacc ctgatagagg gcgatctgcg catccgacat cggcgcgctc
     8161 gccgcaaata cgttgactgt cgcgagcaag cagatagcag cgacaaatgg acccccggcc
     8221 cggcgattgg ctcgcttcag ggacatggtt gcctccctct agaacttacc ttttctgaaa
     8281 aagccgttga atgagatctt cccactcggt cgcttttttc gtattcatcc aaaggtattt
     8341 agcttcaaga tcggtcttct tgaacttctg tggcacgaga gagtcaacgt cgtctttagg
     8401 agagctgaga cccccattca ccacccctgc tgatctttct tggaatgaag atagtttgcc
     8461 ctttttcttt tgctcgttcg aggagaatct gttctcgatt cgcgccctga tagaagaata
     8521 gctccgatgg cgtcaacgat ctcccggctg ggtctaattg ctcgacaact gccaggcgaa
     8581 tgagcagaac caggccaccc taaaacagcc accgaatcgc aggaccgctc tatgccatcg
     8641 gcaacagaag tcattttttc ctcctgatcg aatagtggca atgcgtggtg gcgagtttac
     8701 ttcagcctat tggcatagct ctttaaccaa gccgcgactc gcgcaatgcc gttttccaga
     8761 tcaattatgg gcctccaatc gagttcttcc ttggcgcgag taatatccaa tggatcccga
     8821 tcccgatgcg ctcccacagt cgacgcatgc ggatgcttac cgatctcaaa agagatctcg
     8881 ggcaacgctt ttcggatcga gtcctcgaga tcttggttgc cgtacacctg atcgacggca
     8941 atattatagg cgcggctcaa cggttcttcc gtaaagagcg cacggcgcac ggcatcggtc
     9001 acatcgtcaa tatagatcaa atcatcgggc aagcgggtta ggtagggccg gcaaggctgg
     9061 cccaagacac ccttcttcaa ttccagcgga atccctctgg cgagtcccgg gccatagacg
     9121 gacgagtatc tcaaggcgac gaatggaacc ccgaattttt gtttgtaaaa gtcgcccatc
     9181 cattccgaca tgatcttgcc ggagcgataa aaataagtcg ggtagatcgc cacaggatcg
     9241 tcttctttta tgggtgcgtg atcctttttt cgtagctggc cataaagcgc cccggaactt
     9301 gagtaaacta cgctgctgag ctttaattcc cgggcagctt cgaggatgtt ggtcatcgca
     9361 cgatagcaaa aattcaggac ttcaggacta tcatcgcgcg gaggatgcgc ggcatgaaaa
     9421 atatgcgttg gctgttccaa ccggcaaagc tcccggaaca acgcttcatc ggtgacgttc
     9481 atgatgcggc tgggagtctg gatcgccagc aatgaaaggt ccgtcgagcg cggcaggaca
     9541 tcgatgggaa cgactttgtg gcccagggac tccatcagca gcgttgtatg agagccgatg
     9601 aagccgttgg cgccggtgat gagaatcgta gagtccatgt gtatgctcgg tttttacgag
     9661 caacctgctg ctgaaaaccg tttgcaggct gctcaaaaag acctcagagg ctaggcgcgc
     9721 gaagatcgat gagctgaggc gtacgtccgc agtacgtcga agcgaagcga actagcgcaa
     9781 cgaagcatat gaggcttttt cagcaggctg ctagcgctgg cggatgaaca gcttgcgcat
     9841 aagcccttcc cattcgccaa actttttctc catttcctcg ggagtgtact tgctctccat
     9901 gtaaattttc ttgaactttt gctccagcga accgatgtcg ttgcgcgccg agctaagacc
     9961 gcccttcatg gcgacctgct ggcccgcctt ggaatggaga taatctaaaa acaacaaggc
    10021 ggcatgcggg tgcggcgcat tgacaatcaa gccagagctg cctaccccgg cgataaccgg
    10081 atcgatgggc cgccattcca ccggagcgtt tttttgcttg tgagtaaata tgttggaatt
    10141 gaaaattgtc ggcgaaagcg gcacttcacc cgaagcgacg agcccggaaa gagccgcccc
    10201 ggacatgttc tgcacgttga tttcctgcgc gctcagcttt tccaaaaact cccggcccat
    10261 ggcatcccac agggcgccga tccattgagt tccggtggtg gtcccggcaa tggccatctt
    10321 tcctttccat ttaggatcaa gataatcttt gagcgtcttt ggcgcttcgg cagccggaac
    10381 caagttggtg ttgtacccta ggctgatata aatctcacga ttggtccacg cgagcacgcc
    10441 ggttttgccc tttactttgg cctcatcgtc ataagcgctg agctcgggcg agaagtgatc
    10501 ctgcagcaaa ctcgctcgaa ccaacagggt tacgtattca gggctggttt cgacgacatc
    10561 ggcgaggtac ttgccggcag cgtgctcttc agtgaggcgt ttcagcaacg ccttggaatc
    10621 gctgcgccaa acattggctt tgatgaacgg gtattttttc tcgaactctt gcgacaccgg
    10681 tccggcgacc cacgtattcg atgtatagaa agtgagttgg ccttctttct tggcgccttc
    10741 gacaaggatt ttttcgcgat cagcgccttg atacagcgct aactcaccga ccgatgcggg
    10801 ccggctttgg gcaccctgca agagcggagc atgtgccacg atcgtcaaca aaaggactgc
    10861 gagttttagt ctcctcagca tcgaatcctc ctcggaagca ggtcctcgtc aatcgaaata
    10921 acccgctttg gatgcccaca accagagata ggcaattttt tcattgtaga gctcggaata
    10981 accgacatgc ccgtaccgcg gtataaggac aacgtgcgcg ccgcgggtgc ttgccgcgta
    11041 cttggcgccc atgaacatct cgcgcttgtc ttccagccgc tcgcctttga cccagtggcc
    11101 tttgtcgttt tcaccgacgg ttagcagcac gcgaatcgtc ttcagccaag ccgggtcggg
    11161 ctcttgcaaa tgatcaaagt actcctcgcg cggcaagccg gtgcgtttta cgtattcgtc
    11221 gaggatgccg acggcggcgt tatgctggtt atcgcacagg ctggttttca tctgcgagcg
    11281 cagcggactg atcaggcgga tgtagtcgtc ggctccgccc cacggcgtga gatcttcagg
    11341 atcgacgtaa ccggatgctt tgaaggaatc cggcgagcgg cgcgagacgt gatcgaccgg
    11401 tttttcagag tatttttcgg cgccggtttg attccgccac tccagacgcc aaccatcggg
    11461 gccgccgctg ccgaagccga cgagtccaat gactctggtc tttttggaaa atcgtgtcag
    11521 atgcgcggcc atcggcccac cggtcgagtg gccgaagccg attatttcac gaccggcgaa
    11581 acgctggtcg gtaagaagcc ctgcaccctg aagaatcaca ttgaaagtgc atttcaagtt
    11641 gcgatcgagg atttcttcgg ttggcagttc ccggtccacg agatagatcg gcattcgctc
    11701 cgcaatcggt tgtttccaga cgcccccctt ggggtaatgg cccggatatg tgagagccag
    11761 aacattaaag ccttgggatg caacgacgcg cgccagaccg ggtcttccgt ccggggtaag
    11821 gtccataatt ttttcgctgc cggcgccgcc atggaaaaag acaaaggctc tgttgctgtc
    11881 tagttctttt tccggactga acagcgtccc atgcatatcc cagtcgtagc cattgcggtg
    11941 ataccgggcg gtaacgtctt gggtggatat ctttgtatcg gagtatggcg gatcgatcgt
    12001 aagccattca tcccaattga atttgattaa aagatccgcc gaatccagtt ttttcagaaa
    12061 tttttctatt gctgcagatt ttgccgccat gtcatcctca ttgcttacct acatagttgt
    12121 cccgatacca ggtcgctgtt tcgcccttcg tgcagaacca aacgtccttg aaacccttgg
    12181 cgtatcggat gcactcctcg aacacgccaa taatattggg tctgccgccg atgtgggcgt
    12241 gcacgagcgc atcgatgagc ttgggcgagc ctgcccgccc ctccgcatac aagaggtcga
    12301 aagcggtctt gaagctttca aaataaactc gtgccggatt cttggcgcga aagatgagcg
    12361 aaagatcgtt ggcataatcc agccgcggca tgatgaccag cggtttgccg ttgaccaccc
    12421 gatgctgggg aatgtcgggt cccggtaagt cgccggtcca gagtattcct tcgtctacga
    12481 aatgggcaaa ggtgaattcg ttgattctat ggccggggct aacccatccg atcggtcgct
    12541 tgccggtcac tgaggtaagg ctgtcgagtg tccgcttgat caactctttc tcgcgagccg
    12601 gctcgagcga tatgagagaa gtgtcattga cccaaccatg accgacaatc tcatgcccgc
    12661 cggcgtggag ctctttcaat gcatcgggaa aatcttcggc aacgcggcca ttggtatcaa
    12721 tcgtccctct aacctgatta cgtgccaaga catccatgat ccgccagata ccggctttgc
    12781 cgccgtattc gccgtatgca agcgatgcaa gatccggttt atcgccgcct tcgcgatact
    12841 gcgaatattt ttcaaatgcc tcgaaggcga caaagaagct tacagcgatt tttgcgtcgt
    12901 tgggtaattt gatgggtgcg ggtctcatag tttttacctc cgagttgaaa ccgaggcaga
    12961 tcctatcaag gggttactcg aaccgtcaac ctgaattggc gctggcggaa ataaaacgga
    13021 tgagtttagc agtttgctaa agactttgct ttgcaggctg ctcaaaaagc cctcagaggc
    13081 aaggcgcgcg aagttcgatg agttgaggcg tatgcttcag tacggcgaag cgaggcgggc
    13141 gagcgcaaca aagcctatga ggctttttcg gcagactgtt agtttaccgg gcatttgcca
    13201 gtagtaagat gggcttcgaa atcaccgggg aagagtttga tggcgctttc aatcggcggg
    13261 ccgggcatgt tgattaggtt acaaaagcct ttgcctttgt tgaaggccaa aatcttgccg
    13321 aactgttcaa gaatcggttg accgccttgc ccttgctgca ctttcttcat catcatcgct
    13381 agcgtgctgg tctccatgcg gcaagcaggg cactggccgc aggactcggc ggcgaaaaac
    13441 tcagcgatgc gaagaacttc ttcaacgatg cattctcctt cggcgaccaa acgaataacg
    13501 ccgcagccga tgccggcgcc agcctcacgc attgaattgt ggtccagtgg ggtatcaatt
    13561 ttctccggcg gcaggaatgc gcatgatggc gaggccggca tgaccgcttt gatctttttg
    13621 ccattcttca aaccgccgcc gcactcttcg atcagataac gcagcggcgt gccgaacggc
    13681 agttcataaa cgcctggccg gttcacgtct tcatcaagag caaagagcat ggttccagga
    13741 ctgtcggcgg tgccgaactt tcggtaccac tcggcgccgt gcaaaacgat cggagcaaca
    13801 ttggcgagag tttcaacatt gttcaccgac gtcggtttgc caaatagtcc gaccgttacc
    13861 ggaaaaggcg gcttctgtct gggccaggcc ttcttacctt cgatgacctc cagcacggcc
    13921 gtatcctccc ctgccacata gttgtgcggc gccgggacga tttctatatc caggctgaaa
    13981 ttgctgccga gaacattctc accccagtaa ccctcgctct ttgcttcagc caaagcatcg
    14041 ctaatgctct tcagcgccgt ctcgtactcg gcattaatgt aaagatatgc tttcttcgcg
    14101 ccgatcgcgt aagccgacag gatcacgccc tcgattacca ggtgtggaac attctccatc
    14161 agcacgcgat cttttttgct gccgggttcg tcttctccac cattcatgac taggtagcgg
    14221 ggttgctcgg gcgcctctcg agtaagcgcc cattttttgc ccgt
//
LOCUS       DASUVS010000159         4964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1374252, whole genome shotgun sequence.
ACCESSION   DASUVS010000159 DASUVS010000000
VERSION     DASUVS010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374252"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="VF182_08925"
     CDS             complement(<1..1271)
                     /locus_tag="VF182_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6437242.1"
                     /translation="MSTQCVINLDLADVWDSRGRKSLRRTLAWGDEITVTDQKSTHLE
                     VEVTSFKTASDGSLIPSTRNGFIEPTKSSRIKPADIIVPREQNQVLKVNFVDVQQGDG
                     SIIESPDGKTILVDGGDNQLFARYLAARFRGTSLETPKHIDCVLITHGDADHFSGLVE
                     IYKSETHTNKLKRIFIHPERIYHNGLVKRPGRKDNKPVADRDLLGPTKQIGKKVIITG
                     LVDDLLQVPDEEMNQPFRQWKTALKEYNSRRALSFRRLEFGDSEAFGFFNNSKLQIDV
                     LGPSITESGNVRGLRFLGEPPKAPRVGHASLDLGEEGFAGLSAAHTINGHSVVFRLSY
                     GGFSFLFAGDLNEEASRFLAREHNRGALNLRSEVFKVPHHGSADFSGGFFQAVAPVVN
                     IVSSGDENARKEYIHPRATLMGALGRWSRVP"
     gene            1589..2536
                     /locus_tag="VF182_08930"
     CDS             1589..2536
                     /locus_tag="VF182_08930"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437243.1"
                     /translation="MWKRILIVLVGVFALVNQAAGQSVRGAFPSPSIQILPMMVGAER
                     GFYKREGLDLELVFVRGASTAVQALLANQIHFIFSVGPQMPAVWEGNDIMLLAQQVGR
                     PTFSLVVTPDIQKIADLRGKKIGVTFGGSTAAGTKALLELNKINPDKEVEYISLPGNE
                     PKIAALKQGIISAALLAPPADYLAMKAGMKRLVSLADIFKDTAFTGLAATSRMIKENP
                     QTVKKMVRAMVRSVIHTRDNPEDALQVSMKRLGLDRDAAQDAYQMIRQALVPVPTENG
                     VELMAQWQAIALNTKPKRKPSEYMDLRFVNEVMAELGHK"
     gene            complement(2580..3407)
                     /locus_tag="VF182_08935"
     CDS             complement(2580..3407)
                     /locus_tag="VF182_08935"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6437244.1"
                     /translation="MAFPPNVYVGTSGWTYADWLGSLYPAESKSQDWIHHYARSFRAV
                     ELDSTYSTIPARAVVAGWKEKTPPGFIFAAQVPGVITHQKVMQDCQHELTTFLNSVEL
                     LGDRLGPLLLQFPYFNQNAFASRAQFDKLLQPFLKTLPTEFKFAVEIRNKNWISWDFL
                     EMLRDHSVAFVVVAQAWMPSIDVLARALDLLCADFFYARFIGDRKDGKTQRWDHLAED
                     KTAELRVWIEELKKIADRASRAYVFFSNHYAGFAPGSVELFHDLWHIAAKRCEPEGA"
     gene            3500..4003
                     /locus_tag="VF182_08940"
     CDS             3500..4003
                     /locus_tag="VF182_08940"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6437245.1"
                     /translation="MFSFPLSMEIYNSKEASRIAGVSLRQIQYWDERGFIRPSVKAAR
                     GRGSKRLYSFHDLLCLKVMKDLTHRGLSLRKIRRCLQPLRRYSAEHGRALETLRYLTD
                     GEKLFVITSDPGKIFDAIDRQFVFSVGIGNLVRELNNSLSGGAAATGRANARTMRRYV
                     VKQPGSA"
     gene            4033..>4964
                     /locus_tag="VF182_08945"
     CDS             4033..>4964
                     /locus_tag="VF182_08945"
                     /inference="COORDINATES: protein motif:HMM:NF017039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX6437246.1"
                     /translation="MMQQYLQIKERYRDSILFFRLGDFYEMFFEDAQTASRILDIALT
                     SRHKSEESPVPLCGVPYHAAEPYIQKLLDAGHKVAVCEQIEDPKTAKGVVQREVVRVI
                     TPGTVTAAEALDARDNNFLMAVCQHDRNFGLAVTDITTGEFRCTQFAEDQSLLDEIGR
                     IAPSEIIAPRNSAALLRLLRKEFSNLHITPAPEDYFADEAARKLVAAEDSKSKPGIGR
                     QGLLAASGIINYLEANVGEFLKVIGGLEFYVASHYLVLDENTRRNLELVNGNDGTRKG
                     SLLSVIDRTETPMGARRVRQWLLYPLMEVAAIRER"
BASE COUNT         1135 a         1331 c         1408 g         1090 t
ORIGIN      
        1 tccggcacac gcgaccaccg ccccagggct cccatcaacg tggcgcgggg atggatgtat
       61 tccttacggg cattctcgtc gccgctggaa acgatattca ccaccggcgc cacggcttgg
      121 aagaagccgc cggagaagtc cgcagagccg tgatggggaa ctttaaagac ttccgaccgc
      181 aggttgagcg cgccgcggtt gtgctcgcgt gccagaaaac ggctggcctc ctcgtttaga
      241 tcaccggcaa acagaaaact aaatccgcca taagagagtc ggaaaaccac ggagtggccg
      301 ttaatggtat gggcagcaga caagcctgcg aatccttctt ctcccaagtc cagagaggcg
      361 tgtcccacgc gcggtgcttt gggcggttca ccgagaaatc tcagcccgcg gacattgccg
      421 ctctcggtga tggaagggcc gagaacgtcg atttgtagct tgctgttatt gaagaacccg
      481 aacgcttcac tgtcgccgaa ttcgagccgc cgaaacgaga gcgcgcggcg ggaattgtat
      541 tcctttaatg cggtcttcca ctggcgaaat ggctggttca tctcctcatc cggcacctgg
      601 agtaaatcat cgacgaggcc ggttatgata actttcttgc cgatctgttt agtcggccca
      661 agcagatccc gatcggcgac cggtttatta tctttgcggc cgggccgttt caccagccca
      721 ttgtggtata tccgttccgg gtgaatgaag atccgcttga gcttgttcgt gtgggtctct
      781 gatttgtaaa tttcgaccag ccctgaaaaa tgatcggcgt ctccatgggt gatgaggacg
      841 caatcgatgt gcttgggcgt ctcgaggctg gtgccgcgaa aacgggccgc caggtaacgg
      901 gcgaatagtt ggttgtcgcc gccgtcgacc aggatagtct tgccatcggg agattcgatg
      961 atactgccat cgccttgttg aacatcgacg aagtttacct taagaacctg attttgttct
     1021 cgcggcacga tgatgtcagc cggttttatg cgcgatgatt ttgtcggttc gatgaatcca
     1081 ttgcgcgtgg acgggatcag gctgccatcg gaagcagttt tgaagctagt aacctcaact
     1141 tccaaatggg tgcttttctg atcggtcacc gtgatctcat cgccccaagc cagcgtgcgg
     1201 cgcaacgact tgcgtccacg gctgtcccaa acatcagcca agtcgaggtt aatgacacat
     1261 tgagtagaca tggtcgggtc ctttttacgg cgccggtgat actgataaac gtcgggctct
     1321 cgatcccgcc ccactcgaca gtggcgacag ggccaggcta gttgcgccac ggcgtgcgat
     1381 gcataatctg taggttcgat tacgatctct acgcgaatcg ctctgaaaaa acaaaaagta
     1441 gggatttaaa aactctctgc ggcatgagac gtggtgatgg ctaagcgcaa tcaaaacttg
     1501 gaactcgtgc cgctctctag ataaataaac ttgacgtcag aaatcggccg ttttatggga
     1561 gcggtatccg atgctgcaag gagaaattat gtggaaacgt attctcatcg tgctggtggg
     1621 tgttttcgcg cttgtgaatc aagctgccgg ccaatcagtt cgcggcgcgt ttccttcgcc
     1681 tagtatccaa attctgccga tgatggtggg cgccgagaga ggtttttaca agcgcgaggg
     1741 gctggacctg gagctggtct tcgtgcgcgg tgcgagcacg gccgtacaag cgcttctggc
     1801 caaccagatc cacttcattt tctccgtcgg accacagatg cccgcggtat gggaaggaaa
     1861 cgatatcatg ctgctggctc aacaagtagg acggccgact ttctcgctcg tggttacgcc
     1921 cgacatccaa aaaatcgcgg atctcagagg caagaaaatc ggtgtcacct tcggcggttc
     1981 gacagcggcg ggcaccaagg cgctgctcga gctcaacaag atcaaccccg acaaagaagt
     2041 cgagtatata agcctacccg gtaacgaacc caagattgcc gcgttgaaac agggaatcat
     2101 ttctgccgca ttgctggcgc ctccggcaga ttatctggcc atgaaggcgg gaatgaaacg
     2161 gctcgtgagc cttgccgaca ttttcaagga cacggcgttt accggccttg ccgctaccag
     2221 caggatgatc aaagaaaatc cacaaacggt gaaaaagatg gtccgcgcca tggttcgctc
     2281 tgtcatccac acgcgcgata accctgaaga cgcgcttcaa gtttcgatga agcgcctcgg
     2341 ccttgaccgc gatgccgccc aggacgctta tcagatgatc cggcaagccc tggtaccggt
     2401 gcccacggaa aatggcgtgg agttgatggc ccagtggcaa gccatcgcgt taaatacaaa
     2461 gccgaaacga aagcccagtg agtacatgga tttgcgcttc gttaatgagg tcatggctga
     2521 gctggggcac aagtagggcg cggttcaaaa atgggccgca ggaggcgatc ccggtgagcc
     2581 taggcgccct caggttcgca gcgtttcgct gcgatgtgcc ataagtcgtg aaaaagctct
     2641 accgagcccg gcgcaaagcc cgcatagtga ttgctgaaaa agacgtacgc tcgcgaagct
     2701 cggtcggcta tttttttgag ctcttcgatc cagactctca gctcagcggt tttgtcttca
     2761 gccagatggt cccagcgctg ggttttgccg tcttttcgat cgccgatgaa acgagcataa
     2821 aagaagtcgg cgcagaggag atcgagcgcg cgagcgagca catcgatgct tggcatccag
     2881 gcttgggcga caaccacaaa agccaccgag tggtctctta acatttcgag aaagtcccag
     2941 ctgatccaat ttttgttccg tatctccacg gcgaacttaa attccgttgg caaagttttc
     3001 aaaaaaggct gcaagagctt gtcgaactga gctcgtgaag caaaagcatt ttggttgaaa
     3061 taggggaatt gaagcagcaa cggcccgagg cggtcgccga gaagctcgac gctatttaaa
     3121 aacgtcgtga gctcatgctg gcaatcctgc atcactttct ggtgcgtgat gactcctggc
     3181 acctgggccg caaaaataaa gcccggtgga gtcttttcct tccatccggc caccactgcg
     3241 cgcgctggaa tggtagagta ggtggagtcg agttcgacgg cgcgaaagga gcgcgcataa
     3301 tggtgaatcc aatcctgaga ttttgactcc gctggataaa gcgatccgag ccagtccgcg
     3361 taggtccaac cggaagttcc cacatagacg ttgggcggaa aagccatcaa ttagtgttca
     3421 ccacaagtct agccgatggt caacggaagc aggaatttgt tgactttgtt ggagcggccg
     3481 tttaccttgg aaaccaccct tgttttcatt cccgctttcg atggaaatct acaacagcaa
     3541 ggaagccagc cggatcgccg gcgtctctct tcgccagatt cagtattggg acgagcgcgg
     3601 ttttatccgt ccgtcggtca aggctgcgcg ggggcggggt agcaaaaggc tttactcttt
     3661 ccacgatctg ctgtgcctca aggtgatgaa ggatctgacg catcgaggcc tgagcttgcg
     3721 gaaaattcgg cgctgtttgc aaccgttgag acgctacagc gccgagcacg ggcgagccct
     3781 ggagacactc agatacctca ccgatggcga gaaacttttc gttatcacca gtgatccggg
     3841 aaaaattttt gacgccatcg atcggcagtt cgtattttcc gtcggcatcg gcaacttggt
     3901 gcgcgagctg aataactcac tcagcggggg tgccgccgcc accggccgag ccaacgcaag
     3961 gacgatgcgg cggtacgtcg taaaacagcc cgggtctgcc tgacaacgat ggagtccgcc
     4021 aggatcaccc cgatgatgca gcagtacctg cagattaaag agaggtaccg cgattcgatt
     4081 ctttttttta gacttggtga tttttacgaa atgttcttcg aagacgcgca aaccgcgtct
     4141 cggatactcg acatcgcttt gacctcgcgc cacaagagcg aagaatcgcc ggtgccgctt
     4201 tgcggtgtcc cttatcatgc agccgaacca tacatccaga agttactcga tgcaggacat
     4261 aaggtcgccg tttgcgaaca aatcgaagat cccaagacag ccaagggagt tgttcaacgt
     4321 gaagtagtcc gggtcatcac gccgggcacc gtcaccgccg cggaagcgct cgatgcgcgc
     4381 gacaacaact tcctgatggc ggtgtgccag catgatcgaa atttcggctt ggccgtaacg
     4441 gatatcacca cgggcgaatt tcgttgcacg cagtttgcgg aagaccagtc tttgctcgac
     4501 gagatcggcc gtatcgctcc cagcgaaatc attgcgccgc gcaacagcgc cgcgctgtta
     4561 aggttgttgc gcaaagaatt ctcgaacctc catatcacgc ccgcgccgga agattatttt
     4621 gccgatgagg cggcgcgcaa gttggttgcc gccgaagact ccaagtccaa acccggcatc
     4681 gggcgccagg gtctattagc cgcttctggg atcatcaatt atttagaagc taacgtcggc
     4741 gaattcctta aagtgatcgg cgggctggag ttctatgttg cgtcgcatta tttggtgcta
     4801 gacgagaaca cccggcgcaa cctcgagctc gtcaacggta acgatggaac tcgcaaaggc
     4861 tcgctactct cggtgatcga tcgaactgaa acgcccatgg gcgcgcgccg tgtgcgtcag
     4921 tggctgcttt atccgctcat ggaagtcgcc gctatccggg aacg
//
LOCUS       DASUVS010000160        11599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1593045, whole genome shotgun sequence.
ACCESSION   DASUVS010000160 DASUVS010000000
VERSION     DASUVS010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11599
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593045"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1671)
                     /locus_tag="VF182_08950"
     CDS             complement(<1..1671)
                     /locus_tag="VF182_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6437247.1"
                     /translation="MADLAEVIRRHPLFSALSREDIAKVLGKMEERYYASGATIFSQG
                     DPGDSFYIIQTGAVQVVLENRGRAEIIRVQGPQEWFGEMALLSGEPRSATIVALRDSL
                     IWRLSRHAWDELIDKHPTWLRHFCAALSKKLSYVEQQYSQGREAFYSLAEEFYSSRPA
                     PEQAFLRRLSLLTVADLEVAEEVLPTRIATQCLASMRENHFPLVRFIDSSRLELHPFL
                     VDFLRGKFAQDEGADVAKKVHGELAEQLEELGRWQDAIHHCLAAENWPRAAGLLTRHK
                     QILLDGSADAVRRAIEQLPRDQIDLGLMHMKAEALVRLGRIETAVAYCTEALLDSAKT
                     HRDRATANRLHHVVSSLVLQGDHGQALNLLRNALELRQQDAVSDLASIDEKFDAAGVA
                     APLRQNHSEFIEPNTGASKYLLAAPDRSALSRWIGALIGIGVWSVLWFAEPDIGLEPI
                     AIRQLAFLSLTLIFWMFRVFPEYGVALLFAIGMIISGLAQPEAVLSGFSSSSWFMTLG
                     VLGLGAAITSSGLFYRLSLQLVRVFPLVYKWQIVAMGLMGIVVMALIPQ"
     gene            complement(1717..2691)
                     /locus_tag="VF182_08955"
     CDS             complement(1717..2691)
                     /locus_tag="VF182_08955"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6437248.1"
                     /translation="MLVLSEKQVEALIEIDELIAALERAHIQYSTGKAVMPVRLVVPL
                     PQIDGRITSMPGFLNEDQALGMKVVTYFQNNPKQNLPSILATIMLFSSETGKMIAVMD
                     GSYITAIRTACASAMATKVLANPAASELGVLGAGVQAKAHIAALSQVRRIRSIKIYSP
                     SGTSGSRLKQEVESQLGLAIEVTGSAEEAVRGADLIVIATTAKQPILPADWLKTGAHI
                     NAVGSHRPDLRELDGSTMARAKVFVDSREAIMAECGDVLLALAEKSITADHVQAEIGE
                     VLAGSKPGRTRTEEVTLYKSVGIAIQDVATAQLVYHKALQKGFGTDVV"
     gene            2768..3316
                     /locus_tag="VF182_08960"
     CDS             2768..3316
                     /locus_tag="VF182_08960"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6437249.1"
                     /translation="MANQDHVINLSEVPTVQINAPEGSAFGGSRQRVGAAIGAQKLGY
                     SFFSVPSGKAAFPYHTHTTNEEMIYVLEGRGILRLGREEIAVTAGTIVACPPGTEFPH
                     QLINTGNQELRYLVVSTMEYPDLSEYPDSNKIGAYATAAVGRHVGFRGLFVKDQQAGY
                     YEGEDGQEITRILNSKYRQGAE"
     gene            complement(3345..4022)
                     /locus_tag="VF182_08965"
     CDS             complement(3345..4022)
                     /locus_tag="VF182_08965"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX6437250.1"
                     /translation="MLSYIGPRWQDHLKHRRKLEQLMMNLRQAIALLKRGDVIVFPTE
                     TVYGLGADALNPAAVERVFQLKGRNPDTPIPVIVADQAMLKALVEPIPPLARKLMEQF
                     WPGPLTLVLPAVPSTPKQLLNRTGGVGVRISSAPLATQLARELERPLTATSANPSGEP
                     AASTIEQAQNYFAGDIEIFLDGGQLPSKTGSSVVEVIDDCIKIIREGEISLEHLAATI
                     GRKNILT"
     gene            complement(4095..5360)
                     /gene="purD"
                     /locus_tag="VF182_08970"
     CDS             complement(4095..5360)
                     /gene="purD"
                     /locus_tag="VF182_08970"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX6437251.1"
                     /translation="MKIFVIGSGGREHALIWKLSQSSEVDKIYCAPGSAAIAELAELV
                     RIEPDQIDKLADFATQQKIDLTVVGPELPLTLGISDLFESRGLKIFGPTRQAAQLEGS
                     KSFAKKIMTENSIPTAAAATFSDAQSARRYLEHNRAPYVIKADGLAAGKGVLICANRK
                     EAQWAIDETIVKKAFGQAGEKVVIEEFLEGEEATFMVLTDGDDVLALPSSQDHKRVFD
                     GDQGPNTGGMGAYSPAPVLTETIEDRVIREVVTPLMAGLKRKGIVYRGVIYVGLMITK
                     SGPKVLEFNARFGDPECQVIMMRLKSDLVPLLQATINGTLRTVRPEWYPDPAVGVVLS
                     ASGYPGSYETGKEIRGLEKLTNWPKGFVFHAGTSKETGRWQTSGGRVLVVTARGHNLA
                     EAVQEVYRGVNAISWDGMHYRRDIARRAL"
     gene            complement(5448..7022)
                     /gene="purH"
                     /locus_tag="VF182_08975"
     CDS             complement(5448..7022)
                     /gene="purH"
                     /locus_tag="VF182_08975"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX6437252.1"
                     /translation="MGRIQRALISVSDKKGIVEFSHALSELGVELISTGGTAALLRDN
                     KIAVKDVSEITGFPEMMDGRVKTLHPKIHGGILALRDNPEHVANMREHGIAPIDLVVV
                     NLYPFEATVARGASFDEIVENIDIGGPSMVRAAAKNHEHVGVVVDPEDYPSILAELEK
                     NNGSLSKHTHFRLFTKAFQHTAHYDGAISNYFAALDKNRKSTRWGETVNIQISKIQDM
                     RYGENPHQSAAFYGTEGDGGPSIARAKQFQGKELSFNNILDADAALSTVLEFSDSASV
                     AIKHNNPCGVALSKISLADSFRKAKACDPISIFGGVIAFNRPVDEETAGELKDIFLEI
                     VIAPSFTPEAKAVLSSAKRLLNIRLLELDMSEPQTGGYDLRRVRGGMLMQDWDTGSVD
                     VRACRVVTRRKPTEREYQALDFAWRVCRHVKSNTIVFAAPDQVLGVGAGQMSRIDSTK
                     IAVLRATTHGLNLAGSAVASDAFYPFRDGLDEAANAGAKSVIQPGGSIKDDEVIAAAD
                     EHGMAMIFTGMRHFRH"
     gene            complement(7037..8176)
                     /gene="alr"
                     /locus_tag="VF182_08980"
     CDS             complement(7037..8176)
                     /gene="alr"
                     /locus_tag="VF182_08980"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX6437253.1"
                     /translation="MHDLSPHLGRPTVCYIDHESLRWNFRQIRSLLGPHIKILSMIKA
                     NGYGHGAPAVARTLAAEGSDAFGVAIIEEAIELRRHGITAPILVLTGAYLDQLELFFE
                     HDLMPVVHDPDLLGLLEAAVARHRRSLDVHLKIDTGMGRIGFPAAQCDSWLPQIKKLK
                     AIKLEGIFSHFSHAESVEGQYTRKQLEIFHHLVQRLAAEGIAPPLVHLANSAATITLP
                     EAYFTMVRPGLMLYGVYPSAEMAARILLKPVLSWKTRILQLKKVPSNTSISYGQTFIT
                     KRDSLIATLPIGYADGYPRLLSNRGQALVGGKRAPVAGRVCMDLTMLDVTDIGKIQQG
                     DEVVLLGTQENDAISADEMAAWAETISYEILTSIGARIPRIHHNS"
     gene            complement(8185..8505)
                     /gene="metG"
                     /locus_tag="VF182_08985"
     CDS             complement(8185..8505)
                     /gene="metG"
                     /locus_tag="VF182_08985"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HEX6437254.1"
                     /translation="MISIDAFRQIELKVATVKSAEPHPNADKLMVLQVDLGTEQRQIC
                     AGIRNHYTPEDLVGRQIVVVANLETAKLRGLESQGMLLAASDEERVIFLTPEKPVQPG
                     SKVS"
     gene            8742..9167
                     /locus_tag="VF182_08990"
     CDS             8742..9167
                     /locus_tag="VF182_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX6437255.1"
                     /translation="MGKSLAGSKSHQNLKEAFAGEAQANRRYLYFARIADIEGYRDIG
                     GLFRDTSEAETGHAFGHLDFLKEVGDPCTGVPIGSTEQNLRSAIEGETYEYTEMYPGF
                     AKTARDEGLLELAEWFETLAKAEKSHAGRFSKGLDGLAS"
     gene            9249..9824
                     /locus_tag="VF182_08995"
     CDS             9249..9824
                     /locus_tag="VF182_08995"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEX6437256.1"
                     /translation="MRKVVVDDIVGLAAYEKIRHDSRRRIIELKQKRRVSIGDKVSLV
                     FENRDTVIFQIQEMLRVERISDLDKIREEIAIYNQLIPDSRELSATLFLEIEDQTRLR
                     EELVRFQGIEEALTLHIAGGRHPARFQLGRSKEDKISAVQYVRFRFDDQALEDFVAGA
                     RIELVIDHRNYQAAVMLQPETQRSLAEDLVT"
     gene            9970..10785
                     /locus_tag="VF182_09000"
     CDS             9970..10785
                     /locus_tag="VF182_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6437257.1"
                     /translation="MKRRGFVALLIALVFDPLRSIALAMCNPALVRLQKQGRQLVQVR
                     PGKSPQLGHYFIQWYGHSSFLIHSGSQTKIVADPNFNVTPGIQADAVTVSNDHFTHNN
                     TGAVTGDPVILRGITFRQTWNPIRTSVKDINIINIPSQRGQSWGGAANSIFIYEMGSL
                     CLAHLGNIGHLLTPEQEKVLQRVDVMMVPIDAMTNLGFDDILKVIEQVKPPIVIPMHF
                     DVTRQGELFASFAKERYPVKRFSESQLILSRAMLPKSTEVFVLAHPRAVSLGE"
     gene            10884..11246
                     /gene="dksA"
                     /locus_tag="VF182_09005"
     CDS             10884..11246
                     /gene="dksA"
                     /locus_tag="VF182_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEX6437258.1"
                     /translation="MNKKEVEFFRDLLNQRIQELRSEAGKTVDDMDEDENFPDPSDRA
                     SMESNRNSILRIRDRERKLIFKIQEALQRLEAGEFGICEECGEKIGIERLKARPVTTL
                     CIDCKASQEIAERKAKRM"
     gene            11266..>11599
                     /locus_tag="VF182_09010"
     CDS             11266..>11599
                     /locus_tag="VF182_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX6437259.1"
                     /translation="MKIRKAVIPVAGLGTRFLPATKTVPKELLPIIDIPSIQYVVQEA
                     VDAGIQEIIFVTGRGKDGIEDHFDEAPELEQILADRGQAATVEMLRRIAEMTEVVSVR
                     QKKPLGLGH"
BASE COUNT         2628 a         3110 c         3225 g         2636 t
ORIGIN      
        1 ctgcggaatc aatgccatga cgacaatgcc catgagcccc atggcaacaa tttgccattt
       61 ataaacaagt ggaaagaccc gcacgagttg gagcgataac cggtagaaca gtccggaact
      121 ggtgatggcc gcgcccaatc caagaacccc cagagtcatg aaccatgaac tgctggaaaa
      181 gccactgaga acggcttccg gttgcgccaa accggaaatg atcatgccga tggcgaatag
      241 caaagcaacc ccatattccg gaaaaacccg aaacatccag aaaataagtg tcaaagagag
      301 gaaggccagt tgtcggatag cgatgggttc caagccgata tccggctctg cgaaccagag
      361 aacactccat acgccgatgc cgatcagggc accgatccag cggctcaggg cggacctatc
      421 cggcgctgcc agaagatatt tggatgcgcc tgtgttcggt tcgatgaatt ctgaatgatt
      481 ctggcgcagc ggtgcggcaa cacccgcggc gtcaaacttt tcgtcgatgc ttgccaggtc
      541 ggagaccgcg tcctgctgcc gcagttccaa agcgttgcgc aggagattca aagcctgacc
      601 atgatcgcct tgcagtacga gactgctcac aacatgatgc aggcggtttg cggtggctct
      661 gtcacgatgg gttttggctg aatccaatag cgcttctgtg caataggcaa ccgcagtctc
      721 aatccggccc aaacgaacca gggcttcagc tttcatgtgc atgagaccga gatcgatctg
      781 atctcgtggc aattgctcga tggcgcggcg tacggcatcg gcggagccat cgagcaagat
      841 ctgcttgtgt cgggttagta atcccgccgc tctcggccag ttctcggcgg ccagacaatg
      901 atggatcgca tcctgccaac ggccaagctc ttcaagctgt tcggctaatt cgccgtgcac
      961 tttcttagcg acgtcggcgc cctcatcctg ggcgaatttc ccgcgcagaa aatctacaag
     1021 aaacggatgg agctcgaggc gactggaatc gatgaaccga acgagaggaa agtgattttc
     1081 tctcatcgat gccaagcact gggttgcaat tcgtgtggga agcacttcct cagcaacttc
     1141 gagatcggca acggtgagca gcgaaagccg gcgcaggaat gcttgttccg gcgccggcct
     1201 cgagctgtag aattcttcgg ccagtgagta gaaagcttcg cgcccttgag aatactgctg
     1261 ctcgacgtaa gagagctttt tactcaaagc ggcgcaaaaa tgccgcagcc aagtcggatg
     1321 cttgtcgatc aattcgtccc aagcgtgccg gctcaatcgc catatcaaag agtctctgag
     1381 cgcgacaatg gtcgccgaac gcggttcgcc ggataataac gccatttcgc cgaaccattc
     1441 ttgcggtcct tggacacgaa tgatttccgc cctcccacga ttttcaagca cgacctggac
     1501 agcaccggtt tgaatgatgt agaaggagtc gcctggatca ccttgtgaga aaatggttgc
     1561 gccgctggcg tagtatcgct cttccatctt gcccaatact ttggcgatat cttcgcgcga
     1621 cagtgcggag aagagaggat gtcgtcgtat gacttcggcc agatcagcca tgggggtatt
     1681 tcgccgccgt tttaggcaaa cgggccaatc ttcggatcat acgacgtcag tgccgaagcc
     1741 tttttgtaac gctttgtggt agacaagctg tgcggtggcg acatcttgaa tcgcgatgcc
     1801 cacggattta tagagggtta cctcttcggt tctggttctt cccggcttcg aaccggcgag
     1861 aacttcgccg atttccgcct gcacatgatc cgctgtgatc gacttctcgg ccaaggctaa
     1921 cagaacgtcg ccgcattcgg ccatgatcgc ctcgcgagaa tcgacgaaca ctttcgctcg
     1981 cgccatagtg ctgccatcga gttctcttag gtcgggacgg tgcgaaccca ccgcgttgat
     2041 gtgcgcgccg gttttcagcc agtcggctgg taaaatcggt tgtttcgctg tagttgcgat
     2101 gacgatgaga tcggctccgc gcacggcttc ttccgcgctg ccagtcactt caatcgccaa
     2161 tccgagttgt gattccactt cttgcttgag acggctgccg ctggtgcctg acgggctgta
     2221 gattttgatg ctacgaattc ttcgcacctg ggagagagcg gctatgtgcg ctttggcctg
     2281 gaccccggcg cccagaaccc cgagctctga cgcggccggg ttcgccagga ccttggtcgc
     2341 catcgccgag gcgcaagcag tgcggatcgc ggtgatatag ctgccatcca tcacggcaat
     2401 catttttccg gtttccgaac tgaagagcat gatcgtggcg aggatcgacg gtaggttctg
     2461 cttaggatta ttctgaaaat aggtgaccac tttcattccc agcgcctgat cctcattcaa
     2521 aaagcccggc atgctggtga tccgcccgtc gatttgcggc agtggcacca ccaagcgcac
     2581 cggcatcacc gccttgccgg tggagtattg gatgtgcgcc cgttcgagcg cggctatcag
     2641 ctcgtcgatc tcaatcagcg cttcaacctg tttctcagat aagaccaaca tgatctgatc
     2701 tgatagcata aagatgcggc ggagtggtag tctgcgatta tcaaggatta atcgatcggg
     2761 aggacggatg gccaatcagg atcatgtgat caatctttct gaagtgccca cggtacagat
     2821 caatgcgccg gaaggttcag cattcggcgg ctcgcgacag agagtgggcg ccgccattgg
     2881 cgcgcaaaaa ctgggttaca gcttttttag cgtgccgtca gggaaggcgg cttttcccta
     2941 ccatacccac acgaccaatg aagagatgat ctatgtcttg gaaggccgag gaattctgcg
     3001 cttgggcaga gaagagatcg cagtgacggc cggaaccata gttgcctgtc cgccgggaac
     3061 tgaattccct catcaactga ttaacaccgg taatcaagaa ttgcgctatc tggtagtgag
     3121 cacgatggag tatccggatc tctcggaata tcccgattcc aataagattg gcgcatatgc
     3181 gaccgcggcg gtcggtcgcc acgttggctt tcgcggcttg ttcgtgaaag atcaacaggc
     3241 aggctattac gagggagaag acggtcaaga aattacccgc atcctcaact ctaaataccg
     3301 tcaaggcgcc gaataggtct gcgctggtga ttgcgtcgag ccattcaggt gagaatgttt
     3361 ttcctgccga tcgtcgccgc gagatgctct aggctaattt cgccttcgcg aatgatcttg
     3421 atgcagtcgt cgatgacttc aacaacgctg gagccggtct tcgatgggag ctgcccacca
     3481 tccaggaaaa tttcgatgtc cccggcaaaa taattttgtg cctgctcgat cgtggacgcc
     3541 gccggctcgc cggaagggtt ggcgctggtc gcggtcaaag ggcgttccaa ctcccgagcc
     3601 agctgcgtcg ctagaggtgc gctggagata cgcacgccaa ctccgccggt ccgattcaat
     3661 agctgcttcg gcgtactcgg aacggccggc agcaccaagg tcaggggccc gggccaaaac
     3721 tgttccatga gctttcgggc tagcggcggg attggttcca ccaacgcttt gagcatagct
     3781 tggtcggcta cgataaccgg aataggagta tccggattgc ggcctttgag ttgaaacact
     3841 ctttcaaccg cagcaggatt gagcgcatcc gcacccaatc cataaaccgt ctccgttggg
     3901 aaaacgatca catcaccgcg ttttagcaaa gcaatcgctt ggcgaaggtt catcataagc
     3961 tgctcaagtt tacggcggtg cttgaggtgg tcttgccagc gtggtccgat atacgagagc
     4021 ataaagcgct cgttaaacgt aaatcaattg aagcagacga ggcctaggat gcgcagcggc
     4081 cgggattagt gagcttacaa ggcgcgtcgc gcaatgtccc tgcgataatg catcccatcc
     4141 cagctgatcg cgttgacgcc ccggtaaact tcttgtaccg cttctgcgag attgtggccc
     4201 cgcgcggtta ctaccaagac gcggccgccg gaagtctgcc agcggccggt ttccttgcta
     4261 gtaccggcat gaaagacaaa gcctttgggc caatttgtta atttttcaag cccgcggatt
     4321 tctttgcctg tttcataaga cccgggataa ccgctcgcgc tcaggaccac accgacggcg
     4381 gggtccgggt accattccgg tcggacagtt ctcaacgtgc cgtttatcgt ggcttgcagc
     4441 agcggtacga gatcgctctt gaggcgcatc atgatcacct ggcattcggg atcgccgaag
     4501 cgggcattga actccaaaac tttcggcccg ctctttgtga tcatcaaacc aacgtagatg
     4561 acgcctcgat aaacaatgcc tttacgtttg agccctgcca tcaatggggt gacaacctcg
     4621 cgaatcaccc ggtcttcaat ggtttcggtc aacaccggcg ccggcgagta ggcgcccatg
     4681 ccgccggtat tcggcccctg atcgccgtcg aagacgcgct tgtgatcttg cgatgaaggg
     4741 agagcaagca cgtcatcgcc atcggtgaga accatgaagg tggcttcttc gccttccaaa
     4801 aattcttcga tgaccacttt ctcgccggct tggccaaatg cttttttgac gatggtctca
     4861 tcaatggccc actgcgcttc tttccggttc gcgcagatca gcacgccttt gcctgccgcc
     4921 aagccatcgg ctttgatgac gtatggcgcg cgattgtgct cgagatatcg cctggctgat
     4981 tgggcatcgc tgaaggtagc ggcggcagcg gtaggaatgc tgttctcggt catgatcttt
     5041 ttggcgaaac tcttgctgcc ctccagctgc gccgcctggc gggtaggtcc aaatattttc
     5101 aatccacggg attcgaacag atctgagata cccagggtca atggaagctc gggtccgacg
     5161 acggtgagat ctatcttctg ttgggtggcg aagtcggcga gcttgtcgat ctggtccggc
     5221 tcgattcgga ctaattccgc caattcagcg atggccgcgc tgccgggcgc acagtagatt
     5281 ttatcgactt ctgaactctg gctgagtttc cagatcagcg catgctcgcg cccgccgcta
     5341 ccaattacga atattttcat aatttaaagg attaagaagc taggagcgag aattaatgcg
     5401 agtatcaaac ggccgtcgcg ggactttagg ccggaggcat ctcatggcta atgtctgaag
     5461 tgtctcatcc cggtaaagat catcgccatc ccgtgttcat cggcggcggc gataacttcg
     5521 tcgtctttga tggagccgcc cggctggatc actgatttgg caccggcatt cgccgcttcg
     5581 tcgaggccgt cgcgaaacgg atagaacgca tccgaagcaa ccgccgatcc ggcaagattc
     5641 agaccgtgcg ttgtggcgcg cagcaccgcg atttttgtcg aatcgatacg actcatctgg
     5701 ccggcgccga cgccaagaac ttgatccggc gcggcaaaaa cgatcgtgtt ggatttgacg
     5761 tggcggcaaa ctctccaagc gaagtcgagc gcctgatact ctcgttcggt tggctttctg
     5821 cgggtgacga ctctacaagc gcgcacatcc accgatccgg tatcccaatc ctgcatcaac
     5881 atcccgccgc gtacccggcg cagatcgtaa ccgccggtct gtggctcgct catatccagt
     5941 tccaagaggc gaatattgag caagcgcttt gccgatgaca gaaccgcctt ggcctccggt
     6001 gtaaagctcg gagcaatgac gatttcgaga aagatgtcct tgagctcgcc ggcagtttct
     6061 tcgtcgactg gtcggttaaa cgcgatgaca ccgccgaaaa tcgaaatcgg atcgcacgct
     6121 ttggctttgc gaaaagagtc tgccaaagaa attttcgaca atgccacgcc gcagggattg
     6181 ttgtgcttga tagcgacgct tgcgctatcg gagaattcca ggaccgtgct caacgcggca
     6241 tcggcatcta aaatattgtt gaaggacagt tctttgcctt gaaactgttt ggcccgggca
     6301 atcgagggac caccatctcc ttcagtgccg tagaaagcgg cgctctggtg cggattctcg
     6361 ccgtagcgca tgtcctgaat cttggagatc tgaatgttga ccgtttctcc ccaacgcgtc
     6421 gatttccggt tcttatcaag agcggcaaaa tagtttgaga tcgccccatc ataatgcgcg
     6481 gtatgctgga aggctttggt aaaaagccgg aagtgcgtgt gctttgagag tgagccgtta
     6541 tttttctcta actcggcgag gatcgaaggg tagtcttcgg gatcgaccac gacgcccacg
     6601 tgctcgtgat tcttcgccgc agcgcgcacc atgctgggtc cgccgatatc gatattctcg
     6661 acgatctcgt cgaatgaagc gccgcgggcg acggtggctt cgaatggata gagattcacc
     6721 accaccagat cgattggcgc gattccgtgc tcgcgcatgt tcgcgacatg ctccggattg
     6781 tctctcaatg ccagaattcc accgtgaatc ttgggatgaa gagtcttcac tcgaccatcc
     6841 atcatttcag gaaagccggt gatctcggac acatctttca cggcgatttt attatcgcgc
     6901 agtagcgctg ccgtgccgcc ggttgaaata agctcgacac cgagttcgga caacgcgtgg
     6961 gaaaactcga caatgccttt cttgtcagaa acgctaatca gcgccctttg aatcctgccc
     7021 acgggtagtg atcctcctag gaattgtgat ggatacgggg aattcgagcg ccgatagaag
     7081 tgagaatttc gtaagagatc gtctctgccc aggcggccat ctcgtcggcc gagatggcgt
     7141 cattttcctg ggtgccaagg agaacgacct catctccctg ctgtattttt cctatatccg
     7201 taacatccag catagtcaaa tccatgcaca cccgtccggc taccggcgcg cgcttgccgc
     7261 cgaccaatgc ctgcccgcga ttggaaagca gcctgggata gccatcggca taaccaatcg
     7321 gcagggtcgc gatcaagcta tcacgtttgg tgatgaacgt ctgaccataa ctaatgctgg
     7381 tgttggaagg gaccttcttg agttgcagga tacgcgtctt ccatgacagc accggtttca
     7441 gcagaattcg agcggccatc tccgctgatg ggtaaactcc atagagcatc agacccggtc
     7501 gcaccatggt gaagtaagcc tcgggcaaag taatcgtcgc ggcgctattg gcgagatgaa
     7561 ctagcggcgg cgcaatgcct tcggcggcca gccgttgcac gagatggtga aatatctcca
     7621 gctgcttgcg ggtatattgg ccttcgacgc tttcggcatg ggagaaatgc gaaaaaattc
     7681 cttcgagctt aattgccttg agttttttaa tctgaggcag ccatgagtcg cattgggcgg
     7741 cgggaaatcc aatccgtccc atgccggtgt cgatttttag atgaacgtcg agacttcgac
     7801 gatgtcgggc aacggctgct tccaggagcc ccagaaggtc cggatcatgc accaccggca
     7861 tcagatcgtg ttcgaagaac aattcgagct ggtcgagata agcgccggtg agcacaagga
     7921 tgggcgcagt gatgccatgg cgtcgcagct cgatcgcttc ttcaatgatt gcaacgccga
     7981 aggcgtcgct accttcagcg gcgagagttc tggccacggc cggcgcacca tgaccgtagc
     8041 cattggcctt gatcatggac aggatcttta tgtgcggtcc gagtaacgag cgtatttgac
     8101 gaaaattcca tcgcagcgat tcgtgatcga tgtaacaaac ggtcgggcgg cccaggtgcg
     8161 ggctaagatc gtgcatcctc gtcctcaaga aacctttgag cccggctgaa caggtttctc
     8221 aggagtcaga aagatgaccc gttcttcgtc cgacgcggcc agcaacatcc cttgactctc
     8281 cagaccgcga agcttggcgg tttccaaatt tgcaaccacg acgatctgcc tgccgacgag
     8341 atcttccggt gtgtagtgat tgcgaatgcc ggcgcaaatc tgccgttgtt cggtgccgag
     8401 atcgacctgc agcaccatta gtttgtccgc gttgggatgc ggttcggcgc tcttaactgt
     8461 tgccaccttg agttcgatct gtctaaacgc gtctatcgag atcattccac ctccatcgca
     8521 gcggacacca ggataacaga acagtgcatt caaaaaaaga aagatcgatg aaccggcgaa
     8581 gtcattgccg gtatttattg ttgagataga cggggtgctt tcattccgcg atgtcacagg
     8641 cgatgcgctt caaccagaaa gatacagtga gcttgacagt tgcttgacag ctaaaatggg
     8701 cgttactatc agtagtcgct tgattgaagg gaggattttt gatgggcaaa agtttagcgg
     8761 ggagcaagag tcaccaaaac ctcaaggaag catttgccgg cgaagcccaa gcgaatcgtc
     8821 ggtatctcta tttcgctcga atagccgata ttgaaggcta tcgcgacatt ggtggccttt
     8881 tccgggacac ctccgaggcg gaaaccggac atgccttcgg ccatctcgat tttctgaagg
     8941 aggttggcga tccgtgcacg ggagtgccga tcggaagcac cgagcaaaac ctgagatcag
     9001 ctatcgaagg cgaaacctac gaatacaccg agatgtatcc ggggtttgcg aagactgccc
     9061 gtgatgaagg cctccttgag ctggcggaat ggtttgaaac tctggcaaag gcggagaagt
     9121 ctcacgccgg ccgattcagc aagggattgg acggactcgc tagctgaatt ccctctagcc
     9181 tcttctcggc tctcacggga tttcctcatt tctcgccaag tttccgctta agcggtatcg
     9241 gaagacagat gagaaaagtc gttgtcgatg acattgtcgg cctcgccgct tatgaaaaaa
     9301 tccggcacga ttctcgccgg cgaatcatcg agctcaaaca aaagcgacgc gtttccatcg
     9361 gcgacaaggt atctctggtt tttgagaatc gtgatacggt catctttcaa atacaagaaa
     9421 tgttgcgagt cgagagaatt tccgatctgg acaagattcg tgaggagatt gcaatttaca
     9481 atcagctgat tcctgactcg cgcgaactca gcgcgaccct ttttctcgag atcgaggacc
     9541 aaactcgttt gcgcgaggag ctggtcagat ttcaaggaat cgaggaagcg cttacgctgc
     9601 atattgccgg gggtcggcat cctgcgcgtt ttcaactggg tcgaagcaaa gaagacaaga
     9661 tcagcgcggt ccagtatgtt cgattccgtt tcgatgatca ggcgctggag gatttcgtcg
     9721 ccggcgcaag aatcgagcta gtgatcgatc atcgaaacta tcaggccgcg gttatgctgc
     9781 agccggagac ccaaagatct cttgccgagg atttagtcac gtaacctcga aggcacgctc
     9841 gctcctcaat ccaatctgcc ggactgctct acgcagcctc tcgtatccgg gcaatgtgct
     9901 tgtaacgctc cgggcgcagc gttaaagtgt gaccgctcga cctgcaccac acctttatcg
     9961 ggaggagcca tgaaacgtcg tggctttgtt gctctactga tcgcgttagt tttcgatccg
    10021 cttcgcagta tcgcgctggc aatgtgtaat ccggcgctgg tgcggctgca gaagcaagga
    10081 cgtcagctcg tccaagtcag gcccggtaaa agcccgcaac tggggcacta ctttattcag
    10141 tggtacggcc attcaagttt tctgattcat tccggcagtc aaaccaagat cgttgcggat
    10201 ccaaacttca acgtgactcc tgggatccaa gcggatgcgg tgaccgtcag caatgaccat
    10261 ttcacccaca acaacaccgg tgcggtcacg ggcgatccgg tgattctgcg cggcatcacg
    10321 tttcggcaga cctggaaccc gattcgcacc agtgtcaaag acatcaacat catcaatatt
    10381 cccagccagc gagggcagag ttggggaggg gcggcaaact ccatctttat ttacgaaatg
    10441 ggaagtctct gcctcgctca tttaggcaac atcggtcatc ttctcactcc tgagcaggaa
    10501 aaggtgttgc agcgggtcga tgttatgatg gttccgatag atgcaatgac caacttggga
    10561 tttgacgata ttctcaaggt gatcgagcag gtgaaaccgc cgatcgtgat tccgatgcat
    10621 ttcgacgtca cgaggcaggg agagctattt gccagttttg ccaaagagcg ctatccggtt
    10681 aaaagattct ctgaatcgca gctgattctg agccgagcga tgctgcccaa atccaccgaa
    10741 gtattcgtcc ttgcgcaccc gcgcgcggtg tccttgggtg agtaatagcg catacgaaag
    10801 agtttcgccc ggacttttcc agtcatttga tgtttggatt gcggttacct atactcatgc
    10861 gtgatttgcg aggctgaagt ccattgaata aaaaagaagt agaatttttc cgcgacttgc
    10921 tgaaccagag gatacaggag ctcaggtccg aagccggcaa gaccgttgac gatatggatg
    10981 aggacgagaa ctttcccgat cctagcgatc gcgcttccat ggaatcgaat cggaattcaa
    11041 tcttgcggat acgcgatcgc gagcgaaagc tgatcttcaa gattcaggag gctttgcaac
    11101 ggctggaagc cggcgaattc gggatctgtg aagaatgcgg cgaaaagatc gggattgaac
    11161 gcctcaaggc gcgtccggtg acgactctct gtatcgactg taaggccagc caagaaattg
    11221 ccgaaagaaa agccaaacgg atgtaaggat cgatctggcc aacctatgaa aattagaaaa
    11281 gcagttatcc cggtagccgg cttgggtacg cgttttctac cggcaacgaa aaccgtgccc
    11341 aaggaacttt tgccgatcat agacattcct tcgatccagt acgtggttca ggaagctgtt
    11401 gatgccggta tccaagaaat catctttgta accggccgcg gcaaggatgg catcgaggat
    11461 cattttgacg aggccccgga gttggagcaa atcctcgcgg accgcggcca ggcggcaacc
    11521 gtggagatgc ttcggcgaat cgccgagatg accgaggtcg tttcggttcg gcaaaaaaaa
    11581 ccgcttggcc tgggtcacg
//
LOCUS       DASUVS010000161         7490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_719451, whole genome shotgun sequence.
ACCESSION   DASUVS010000161 DASUVS010000000
VERSION     DASUVS010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719451"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1089)
                     /locus_tag="VF182_09015"
     CDS             complement(58..1089)
                     /locus_tag="VF182_09015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX6437260.1"
                     /translation="MINRRTLVLFPGALGDFVCFLPVLDYLAERWSIDLLARTEFSEL
                     VPGRVKVMSLERFEIRHLFVSGGGNNGQVRSLFETYDCTYSWMGSRDPTFKQELEAAT
                     QGRLRLFPFQPRDSKLHQVDYYLSCVQVDSVPGMPRIPLMPDAKQSAEVYWRKNSLNT
                     KPVLAIAPGSGAREKNWPAISFEAIARWWREQVGGSVVVILGPVEEERGGTESLCSHG
                     IAIRDIGLARLAATIARCSLYIGNDSGLSHIAAAIGVPTAAIFGPSDVNRWAPRGETV
                     LVLRREQHCSPCAVPAMKDCPHHDCLTALTSAEVIRQLERWPPLLTLTRRGAGIRVPA
                     EISPDLMGG"
     gene            1196..2140
                     /gene="cysK"
                     /locus_tag="VF182_09020"
     CDS             1196..2140
                     /gene="cysK"
                     /locus_tag="VF182_09020"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX6437261.1"
                     /translation="MATLNGAGSILDLVGSTPVLKLKNLPGRDDAEVWAKLEAFNPGG
                     SVKDRICSNMIETAEREGKLKPGSTIIEPTSGNTGIGLALVAAVKGYRLVLTMPDTMS
                     EERRSLLAAYGARLILTPDTKGMGGAIHKAEELVQENSDYFMPQQFNNPVNPETHRRT
                     TAMELLKQFKRIDAFVAGVGTGGTITGVGEVLKDRLKAVRICAVEPASSPVISGGEPG
                     YHKIQGIGAGFIPAVLNRQVIDEIIPVSDTEAINYTRRLASEEGLLLGISSGAACCAA
                     LRVAKTLGKGQIVVTVFADKGEHYLSTDLFGEKRWTQS"
     gene            2125..2937
                     /gene="larE"
                     /locus_tag="VF182_09025"
     CDS             2125..2937
                     /gene="larE"
                     /locus_tag="VF182_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEX6437262.1"
                     /translation="MDAKLNNLETLLRETNGAIVAFSAGVDSTFLLKIAHAVLGERAI
                     ALTAASPTVPPGELEVAKEFARTLGCRHIVIDSNELANPSFAQNPSNRCFFCKDELYR
                     LCREQAQKLEVPVIVDGTNLDDLKDHRPGLKAAKEWGIRHPLLEAEMTKEDIRRQSRA
                     LDLPTWDKPSSPCLSSRFPYGTEINLERLRKIGACEVYLKELRFREFRVRYHGDLARI
                     ELSAQEIARFFDERTRQAVVKKFKEIGFNYVSLDLQGYRTGSMNEALASKTI"
     gene            complement(2934..3629)
                     /gene="ispD"
                     /locus_tag="VF182_09030"
     CDS             complement(2934..3629)
                     /gene="ispD"
                     /locus_tag="VF182_09030"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX6437263.1"
                     /translation="MNATAIIAAAGEGRRVGADISKVYLPIGGRAVLLRTLDQFFQIK
                     TIKNIIVVIAKQDSERCQSLLSRDPRAGAQSWILQTGGSSRQKSVARGLERVSSECDV
                     VIIHDGARPFVSPGLIDRVIKEVPARKAVVVGVPARDTIKVVSESRQILSTPARDRLW
                     EIQTPQAFEPSLILEAHQAAQGNDQDATDDASLVERLGKPVYLIEGDRSNIKITLPED
                     LLFAEALLASAST"
     gene            complement(3708..4190)
                     /locus_tag="VF182_09035"
     CDS             complement(3708..4190)
                     /locus_tag="VF182_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX6437264.1"
                     /translation="MFKVGEKVVYPAHGVGEIEAIRIHVISGSERKFYMLRILETDMK
                     IMIPIENVDSVGLRKVIDRAMVSKVYKVLRQKKVETDQQTWNRRYREYTEKIKTGSIL
                     EIAKVLRDLFVLKGDKELSFGERKMLDTARNLLVKELSIARSHSEEKILEELRHIFTH
                     "
     gene            complement(4563..4985)
                     /gene="rimI"
                     /locus_tag="VF182_09040"
     CDS             complement(4563..4985)
                     /gene="rimI"
                     /locus_tag="VF182_09040"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX6437265.1"
                     /translation="MTTADIESVIAIERASFQFPWSTRFFLDELQVDCARSILAEIES
                     RIVGYVLFWFLPEDVDIHNIAVHPDFRRQGIGRLLLEQVIVAARRQERLRVTLDVRFS
                     NTPAQNLYKSFGFVTRGLRKGYYSDNGEDALVMALELR"
     gene            complement(6235..6480)
                     /locus_tag="VF182_09045"
                     /pseudo
     CDS             complement(6235..6480)
                     /locus_tag="VF182_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741041.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
     gene            6590..6665
                     /locus_tag="VF182_09050"
     tRNA            6590..6665
                     /locus_tag="VF182_09050"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6623..6625,aa:Cys,seq:gca)
     gene            complement(6684..6953)
                     /gene="grxC"
                     /locus_tag="VF182_09055"
     CDS             complement(6684..6953)
                     /gene="grxC"
                     /locus_tag="VF182_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEX6437266.1"
                     /translation="MAQVVMYTTSWCPYCRAAKSFFASKKVDFTEIDVTEEPKLRVEM
                     ERLSGRRTVPQIFIGGKPIGGYDDALSLEASGELEKLLNGTPGTS"
     gene            7032..>7490
                     /gene="coaE"
                     /locus_tag="VF182_09060"
     CDS             7032..>7490
                     /gene="coaE"
                     /locus_tag="VF182_09060"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX6437267.1"
                     /translation="MKLIGLTGGIASGKSTVSAILRRLGAKIIDADALARQIVEPHQP
                     AWNEIVDNFGKEILQADGTLDRKKLRKLVFDEPNARKQLEAMTHPRIRRLAQEKIAES
                     AATEAPLVVYEAPLFFETGIHVWLRPVILVAADTATQKRRLQERDNLTEAE"
BASE COUNT         1850 a         1988 c         1898 g         1754 t
ORIGIN      
        1 cttaccagct atatctgatc attcactgcg taagcctgaa gcgatccccc aactgcccta
       61 tcctcccatt aaatcaggcg aaatctcagc aggaactcta atccctgctc cgcgccttgt
      121 caaggtaagc agcggcggcc agcgctcgag ttgtcgaatg acttccgcgg aggtaagcgc
      181 agttagacaa tcatgatgcg ggcagtcttt cattgctgga acagcgcatg gagagcagtg
      241 ctgctcacgt ctgagcacca agactgtctc tccgcgcggc gcccaccggt taacatcaga
      301 agggccgaaa atagctgcag tgggaacccc gatcgctgca gcgatatgag atagaccact
      361 gtcattgccg atataaagac tgcagcgcgc gatcgttgcc gccagacgcg ccaatccgat
      421 gtcacgtatc gctatcccgt ggctgcagag agattcagtg cctccgcgct cttcctccac
      481 cggtccgaga ataacaacta cactgccacc aacctgctct cgccaccagc gcgcaatcgc
      541 ttcgaaagag attgccggcc aatttttctc gcgcgcgcca cttcccggag cgatggcgag
      601 aacaggcttc gtattgagcg aattcttcct ccagtagact tctgccgatt gcttggcatc
      661 cggcatcagc ggaatgcgtg gcatccccgg aaccgaatcg acttgaacgc aggagagata
      721 gtaatccacc tgatgcagct tcgaatccct cggctggaat ggaaatagtc ggagccgacc
      781 ctgagtagct gcctcaagct cctgcttgaa ggttggatct cggctgccca tccaagaata
      841 agtacagtca taggtttcga aaaggcttcg cacctgccca ttattccctc cacccgagac
      901 aaatagatgg cggatttcaa agcgctccag cgacataacc tttacgcgcc cgggaacaag
      961 ctcagaaaat tctgtccggg ccagcaaatc aatcgaccat cgttcagcca agtagtcgag
     1021 gacggggagg aagcagacga agtcgcctaa agccccggga aagagaacta gcgttcgtcg
     1081 attgatcact tacagtatgc gctccgtttg catttaattg actttatttt ggcaagtata
     1141 tagcataccc gcaaatcttc gagttttgct cccgcttagg aagtcgagca caccattggc
     1201 aacgttaaac ggcgcggggt ccatcctgga tctggtcggc tccacacctg tcctcaaatt
     1261 gaaaaacctg cccggcagag atgacgccga agtttgggcc aagttggaag cgttcaatcc
     1321 cggcggatcg gtgaaggatc gcatctgctc caatatgatt gaaaccgcgg aacgtgaagg
     1381 caagctgaaa ccgggatcaa cgattatcga acccactagc ggcaacaccg gcattggtct
     1441 cgcactggtt gcagccgtga aaggctatcg tctggttttg actatgccgg ataccatgag
     1501 cgaagaacgc cgcagccttc tggccgccta tggcgcgcgt ttaatcctca ctcccgacac
     1561 taaaggaatg ggcggcgcta ttcataaggc cgaagaactc gtgcaagaaa atagtgacta
     1621 ctttatgccc cagcaattca acaacccggt caacccggaa acgcatcgcc gtaccacggc
     1681 gatggaactg ttaaaacagt tcaaacggat tgatgcattt gttgccggcg tcggcaccgg
     1741 gggaaccatc accggcgtcg gtgaagtgct gaaagatcgc cttaaagctg tgagaatctg
     1801 cgccgtggag ccggcttcat cgccggtcat ttccggcggt gagccgggtt atcacaagat
     1861 tcagggaatt ggcgccggtt tcataccggc tgttctgaat cgtcaagtta ttgatgaaat
     1921 tattccggtc agtgatacgg aggcaatcaa ctatacccgc cggttagcgt ctgaagaagg
     1981 tcttctgctc ggcatatctt cgggcgcggc ttgttgtgcc gccctccgcg ttgccaagac
     2041 cttgggaaaa ggccagattg ttgttactgt cttcgcagac aaaggcgaac actatctaag
     2101 cacagatttg ttcggggaga agcggtggac gcaaagttaa acaatttaga aaccctcctg
     2161 cgcgaaacca acggcgcaat tgtggcgttc tctgccggcg tcgattcgac atttctactg
     2221 aagatcgctc acgctgtctt aggggagcgc gcaatcgctt tgaccgcagc gtcaccaacg
     2281 gtgccgccgg gtgaattgga agtcgccaag gagttcgcca gaacgctagg ctgtcgccac
     2341 atcgttatcg actctaacga attggccaat ccctcattcg cacagaatcc ttcaaatcgg
     2401 tgcttcttct gcaaggacga gctttaccgg ctctgtcgcg agcaggcgca aaagttagag
     2461 gttccggtca ttgtcgacgg taccaatctg gacgatctaa aggatcatcg tcccgggttg
     2521 aaagcggcga aggagtgggg cattcgtcac ccgttgctag aagcggaaat gactaaagag
     2581 gatattcgac gccagagccg agcgcttgat ctgcccacct gggacaaacc ttccagccct
     2641 tgtctctcat cccgttttcc ctatggaacc gaaatcaatc tcgagcggct gcgaaagatc
     2701 ggcgcgtgcg aagtctattt gaaagaactc cgttttcgcg agttccgggt gcgctatcat
     2761 ggcgatctgg cgcgcatcga gttgtcggcg caggaaattg cccgattctt tgatgagagg
     2821 acgcgacaag ccgtggtaaa aaaattcaaa gagatcggtt tcaactatgt gagccttgat
     2881 ctacaaggct accgcacggg aagtatgaac gaggcgctgg caagcaagac tatttaggtc
     2941 gacgcgctgg caagcagcgc ctcggcgaaa agcaaatctt ccggcagcgt gattttaata
     3001 ttacttcgat ccccttcgat caaatagaca ggttttccca aacgctcgac caggctagca
     3061 tcgtcggttg cgtcttgatc gtttccctgc gccgcttgat gcgcttcaag aatcaagctc
     3121 ggctcgaacg cttgaggcgt ttggatttcc cataagcgat cgcgggccgg agtcgagagt
     3181 atttgccgac tttcagaaac cactttgatc gtgtcccgcg ccggtacacc cacgacaacg
     3241 gcctttcgcg ccggcacctc tttgatgact cgatcgatca ggccagggga gacaaacggc
     3301 cgtgccccat catgaataat tacgacatcg cactcggatg agaccctttc gagcccgcgc
     3361 gccacggatt tctgccggct cgatccgcct gtctgtagaa tccatgattg cgcgcctgca
     3421 cgtggatcac ggctaagcag agattggcac ctttcagaat cttgtttcgc aatgacaacg
     3481 atgatgttct tgattgtttt aatctggaaa aattggtcca atgtccgaag caaaacagct
     3541 cggccgccaa tcggcaggta gactttgctt atgtcggcgc cgacccggcg tccttctccc
     3601 gccgcagcta tgattgcggt cgcgttcact gcgtgtgctc cagctaaaac aaaaagagcc
     3661 gagagattaa ctcccggctc ttttgtcttg cagcatgtag gttcagttta gtgagtgaag
     3721 atatggcgca actcttcaag aattttttcc tcggagtgag agcgcgcaat tgaaagttct
     3781 ttcactagga gattgcgagc ggtatccaac atctttcttt caccaaaaga taattcttta
     3841 tcgcctttaa gcacaaagag gtcccgtaat accttagcta tctcgagaat cgagccggtt
     3901 ttgatctttt ccgtgtattc acgataacgc cgattccatg tttgttgatc cgtttcgact
     3961 tttttctggc gcaagacttt gtagaccttc gaaaccatcg cgcgatcgat aactttccgt
     4021 agtcccacag aatctacatt ttcgatgggg atcataatct tcatgtcggt ttccagtatt
     4081 cgaagcatgt agaatttcct ctcacttccg gaaataacat ggattcgaat ggcctcgatc
     4141 tctcccactc cgtgagcggg ataaactact ttttcgccaa ccttaaacat agtgacctcc
     4201 tcgtgaacga tagcgtatag ggcgtctctt cgagattaag cagatctgta aggggacggg
     4261 gacgagagct gaagtggaga ggattaaaga attgaagcgg atttgattgc agaaacagct
     4321 aggatggggt cggcggctgc ttcttattaa cattcgttat accgtttcat atcttcagcg
     4381 ccatcaaata gaattggcgg tcaggggttc gaccgccaat ttgccagaca ccttagttat
     4441 acaacagagc tcacagcagc gtcaagagag ctccaatatt tcttaaaatg ccaaaataat
     4501 ccgtatctta cgcctaagta taactggtgc tggccatacc ggcgaaatta cgatcgcagg
     4561 gtttatcgca gttctaaagc catcacgaga gcatcttccc cgttgtcgga ataataacct
     4621 ttccgcaagc ctcgtgtgac aaatccgaag ctcttataaa ggttttgtgc tggcgtatta
     4681 gagaacctca cgtcaagagt tacgcgaagc ctttcttggc gccgcgctgc tactatcacc
     4741 tgctccagta gaagccgacc gatcccctgg cggcgaaaat ccggatggac ggcaatgtta
     4801 tggatatcta cgtcttcagg tagaaaccag aacagaacat aacccacgat acggctttcg
     4861 atttcggcaa gaatggaccg ggcgcagtcc acctgtagtt cgtcaagaaa gaaccgggtg
     4921 ctccaaggaa actgaaaaga tgccctctct atggcaatca cgctttctat atctgcggtg
     4981 gtcattgacc gaaccgaagc ggttttctcg ggagcaaaaa agccacaagg tgcttcaccc
     5041 tgtggctcag gccgattaag aggaaaagtt cgggctgact tatttttact gataggtcgc
     5101 tcccttaaga atgctttgat cctttaacag aaggacttga agcagcttgt tttcgaattt
     5161 gtggacaaac ccgacgattc gatcttgcag cggaattacc gtcgtaaact ccggcggaaa
     5221 cgcgcccaca ctggaggaaa tttttctgac ccgctcgtca atgcacttcg ctcgaactat
     5281 gaattggccg tttttaaggg cgagatcata atcgtaatcg ggcatttcat cggaaatttt
     5341 taaacgatcg gcaaccccga ggtcgggaat cgatcgcgga aattcaagcc gcaacagcaa
     5401 tgccccgcct cgatcttcaa cagtataaac attgccgtag cgtctttcgc gttcgagctt
     5461 gtcgacgaaa ccgcgagagt aaaagccgtc gaccggaatg ggcagattgc ggaccatacc
     5521 accctgtctc gccaaaaagg gttgcaataa gacggctagc ggaatcccgt aaatggctgc
     5581 tgggaaattc atctcttctg gaggcttggc gtgaaagatt ccgtccctca accgaaagat
     5641 tccttcgatc aacgcgagaa tgataccgac gaatacgaac atattgatgc tttggctgaa
     5701 gagaactgac ggcccgttga ccaccgacgc cactaccatc agaataattg gatataatac
     5761 cgcgttcaga agaatgttca gacctgtgct gccggcagcg ctgaaaacaa taggactttg
     5821 taccgttcct tccagggttt tcttcgtctt atgcggtaaa gcaccgagca caggttgaca
     5881 aaatccaatc aacaaacgat taagtcggct taactttccg acagcgcgaa actgcagggc
     5941 aagttcttca aagtcgtcag gcagttgatt gggaaaaatt ttcttcggct cagcttcctt
     6001 cttaggcgcc ggcgcgggtt gtgggggcga tttggcaata atagccggag tgaccgccgg
     6061 cttggacgct gcggcaggag cagccactga cggcttcgcc ggaggagtcg ttgcggcagc
     6121 gacgacagga gcaataggcg gcggcacgct cttctcagag atatccacag cggcagcgag
     6181 atcagctcca ttggttttgc gaaatcgtga ctgaaaacta tcgccgaagc tggtttccgg
     6241 gtgtagcgct ttggcgcgag caatcagagc ttcttcgctc tctatgttgt taggatctgt
     6301 tacacagcag tcgactggac agacagccgc gcacgcctcg aaatcatgaa atccaacgca
     6361 ctcggtgcat aaaagcgggt caatgacata aatgggatca ccctgactga tcgcattatt
     6421 tggacactca ggctcgcatg ccccgcagtt gatacactcg ctggttatca tcgttgccat
     6481 taagaaaaac tcctggtctg gtgttaatta cgtgcttacc ctattcttgt caatataaac
     6541 acaggcgttc ttgcatgtaa gagaaagatc atgttagcta tcgcttcaag gcggcgtacc
     6601 caagtggtta agggagaggt ctgcaaaacc tcgatgcagc ggttcgaatc cgctcgccgc
     6661 ctccagtgat ggacatcacc gaatcaggaa gttcccggtg tcccatttag tagtttttcc
     6721 agttccccgg aggcctctaa actcaaggca tcgtcgtatc cgccgattgg ctttccacca
     6781 ataaagatct gcggaacagt tctccggccc gataaacgct ccatctcgac tctgagcttt
     6841 ggctcctcgg tcacatctat ttcggtgaag tcgaccttct ttgaagcaaa aaacgacttc
     6901 gccgccctac aatacggaca ccagctggta gtatacatga ccacttgcgc cataaacgct
     6961 cccatgcgaa cctgaacatc ggtttcggct gtttcgttgc aacctcctat ctattttgcc
     7021 attactgttc cgtgaaatta ataggcctaa ccggcggtat cgcttcgggt aaaagcaccg
     7081 tctcggctat tctccggcgg ctcggggcca aaatcatcga tgccgatgcg cttgcgcgcc
     7141 aaattgtaga gccgcatcag cctgcctgga acgagatcgt cgataacttc ggtaaggaga
     7201 ttttgcaggc ggacggaaca cttgaccgaa aaaaattgag aaaacttgtc ttcgatgaac
     7261 cgaatgcacg aaaacagctg gaggccatga cccatcccag gattcgtcga ttggcgcagg
     7321 aaaaaatagc agagtcggct gctaccgagg cgcctcttgt ggtttatgaa gccccgctgt
     7381 tcttcgaaac tggcatccat gtttggctcc gccccgtcat tctggtggct gccgacactg
     7441 ccactcagaa acggcggctc caggaacgag ataatttaac cgaagcggaa
//
LOCUS       DASUVS010000162        12691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1811707, whole genome shotgun sequence.
ACCESSION   DASUVS010000162 DASUVS010000000
VERSION     DASUVS010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12691
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811707"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VF182_09065"
     CDS             complement(<1..416)
                     /locus_tag="VF182_09065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6437268.1"
                     /translation="MPTVKVNDVEIYYEEHGKGRPMVFLSETACDGEVWKIHQVEEFS
                     RDHRVIIHDYRGTGQSSKPSIDYTTTMFAEDVVALMDHLKVENAIVVGHSMGGRVAQL
                     LALDHPNKVHKLVLASTGAHYPQTKGLPLKICKEMI"
     gene            573..1586
                     /locus_tag="VF182_09070"
     CDS             573..1586
                     /locus_tag="VF182_09070"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437269.1"
                     /translation="MKEIAFPYSILRICVFLLLCAPGLVLAQQPAKLAFPYGPLGLNS
                     VPWIVAKEIRIFEKNGLDPDMVYVGASAVIVQSMLSGSSNVAGFGGPAVITNVLRGGD
                     IIQIAAMAPYFTQSLFVRPDIRDLRSLEGKRIGITRFGSVTDFALRSIIERHNIKGVN
                     VLQMGGFPEAVAGLSRGAIDGAVFSPPHNFRMLKEGFRELVSQKDLRAFGSGFLTQGI
                     VARRSFAATHREIIVRLIKSTVEGTKYAVTNEDSTKRIIGKYLGISDGDLLRQSYLYV
                     VDTFVREPFVPESVMQAMVQRMVEVNMIDVKSAEVTPISAYFDNSYVAELKQSGFLDS
                     IWK"
     gene            1673..2692
                     /locus_tag="VF182_09075"
     CDS             1673..2692
                     /locus_tag="VF182_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437270.1"
                     /translation="MPRWIRVGFSVAVPLVVFFVATAGAQDRVRLGLSSISATSGSIW
                     VAEEKGLFKKHGIDAEVIIIGGGAARVVSSLIAGEIQFSVGGGDAVIRAALRGADGVL
                     VASPMKTGLQRLMVRPEIKTPADLKGKTIAVTRFGSASHWVLQLFLRKWGMRPDDIKM
                     LQLGSSPAMFASLEKGAVDGGAFTIPTFFLAEERGYRILADPVDMEIYYLQNSIDSTR
                     GYIKRNRDQSLRLIKALCEGLAYFRKNKKESMEIMQKKLRIQSAQERDVKYLELSYNL
                     LADKFYNRVPYATPKAVETTLEFIAGEEPKAKGADPKQFIDESLVREVEASGFIKKLY
                     DTDYR"
     gene            2794..3675
                     /locus_tag="VF182_09080"
     CDS             2794..3675
                     /locus_tag="VF182_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6437271.1"
                     /translation="MADYKEILYTKQRGGALITLNRPEALNAITRPMLKELHHALDEA
                     EADPDVRAIVITGAGRAFSSGFDQTTTTGRRRDIQWPYGIQTGMSAADLMNYWSADDV
                     KFIRIFEMTKPVIGAVRGWAMGGGCWLALYTHITIAAEDAVFAQPEVRHGSNTTFMWT
                     LLAGFKNALRYGLVGDHVDAHEALRIGLVNKVVPVDQLLEESFKIVERISHVPPETVK
                     INLQISTMGLQMMGLRDALRMDETLSIPAHMMLNEEFRRPLDEARANQGTKAYLQLRD
                     GPFQPEPFGPRSKKKTG"
     gene            3753..5930
                     /locus_tag="VF182_09085"
     CDS             3753..5930
                     /locus_tag="VF182_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX6437272.1"
                     /translation="MTHSTSSGTAVAWRDLSPLMNPRSVAIIGASQRSASALNREPRG
                     NRVIRNLKSFGYAGRIVAVNPKYSEVMDYPCYPEIGAIPEPVDCVVLAVPNRQVPDLL
                     ESAAIAGVRAAVVFSAGFAETGAEGKQRQARLEALSRERGFLICGPNCYGVLNVFGKA
                     PLFASTIPQGFLGGPVALVSQSGGLSTTIANALMLNRHVGLSHIVSCGNQAGATVEEY
                     FNYLVEDENTRVIAAFVEGFKQPNLLLAVARKAAERNKPLIILKGGRSEVSQRAAATH
                     SGSLAGAAEVIDAAFRQSGVVSVRSINELIDATSVFSCDGFVKHYCGGRRIGVLSGSG
                     GECTLVSDAAASVGLEVPELTQSTKSQLQEAVADFGNMNNPLDGTGAMYDDEKIFPRL
                     LQALIDDANIDVVTINLEANDPRPKELKSGNRFAQAIERAAASSKKPIACFSSVVGGP
                     VDPEILLPLRQGGVPLMEGAECATTTIRNLAEFYEFQQRRQSEATGDQPAPGRKIASG
                     VLPAQAAFQLLEEFGISTVPMTLAHSPEEAAAAAEKMGFPVALKIESAQITHKSDVGG
                     VALKLSDTAAVSDAYNKIMSEVRRRAPDAKIEGIVVQRMSGQGVEMILGIKRDPLFGP
                     VVLCGFGGILVELLKDVSIGIPPLSRKQAHDMLKRLRGFPILGGVRGQAATDIDALCD
                     AIAGLSRLAWSFGDQLAGLDINPLIVLPKGGGVVAVDALVEIR"
     gene            6075..7061
                     /locus_tag="VF182_09090"
     CDS             6075..7061
                     /locus_tag="VF182_09090"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437273.1"
                     /translation="MNRSLPLIIITVWAVLFLPGLTLAQSKPLKEVRVPYALGGSTGF
                     FWVAQRSGSFEKYGLKVLPIFMRGGREAVQALISRDVTMLQQGSSGVILAWAQGAKDL
                     VVIGATGNKLDYVLVSSAAIKKPADLKGKKIAISQLGAGTDFIARHAVRQLGLNEKDV
                     TLLSIGAQGERWAALAGGHVDASVFQPPVTLRARKAGLPIWVDFSKTEYEYVVAGPVT
                     TRSFIKTDRETVMNFMRGLADGMDFYRDEKNKDAVIKYLGEFYKSTNTEELEETRQVY
                     RQVTPGLPIVTLKSMENMIASDRMLSTMNINGADVMDLSFLRQLEEERKTKK"
     gene            7166..8047
                     /locus_tag="VF182_09095"
     CDS             7166..8047
                     /locus_tag="VF182_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6437274.1"
                     /translation="MPDYKEILYEKRRGGALITLNRPEAMNAISRSMIKEIHQALDDV
                     EEDNEVRAVVLTGAGRAFSAGMDQGNRAGRRRDIHWPYGILTGQSAAEVIDSWRVDPR
                     NFRRMWEFGKPIIGAINGWAMGAGSWLSLFTHITIAAENAVFAQPEVRHGSNTSFMWT
                     LLGGYKNALRYGLTGDHIDAQEALRIGLVVKVVPPEQLIDECFNLVERIAHVPPETVK
                     INLQVATMGLQMMGLRDALTFDSQMSAPAHIMLREELRKPLDEARTNKGIREYLQMRD
                     GPFQPEPFGPRSKKKTE"
     gene            8127..9005
                     /locus_tag="VF182_09100"
     CDS             8127..9005
                     /locus_tag="VF182_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6437275.1"
                     /translation="MTNYNTILYQKQRKGVLITLNRPNALNAMNQDLMNELDQALSEA
                     EIDPEIRAVVITGADGAFSVGEDISGDDPETAWPYGIPAGTSLNATYNKFRDGDRKDI
                     LGRQAYRWQYPKPIIGAVSGWCLGAGSWLALTCHITIAADDAVFGQPQVRHGANTDFI
                     WVALAGFKNALRYSLTGDHVDAKEALRIGLVNQVVAKDQLLETCFQFVERVAHVPPET
                     VKINLHISTLGMEMMGLRKAWTLNAELAAMARLTKREEFNKRLEDAKKKGGLAAFLQE
                     RDAPFQPEPFGPHAKK"
     gene            complement(9432..10475)
                     /locus_tag="VF182_09105"
     CDS             complement(9432..10475)
                     /locus_tag="VF182_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6437276.1"
                     /translation="MKIFGVALLILVTTSSLGWGQTKKPRTLDELTAYNGVDRHQILL
                     DGAKAEGKVVWYTSLSGVYRELVDAFKRKYPDVAVEVYRGGSTDLGPRLLNEAQAGRH
                     VADALESTPGLLMLLRERGLLKAYTSPELARYPDEAKTKADGPRIYWVTDREAYLGFG
                     YNTRQIAPAEIPKNFQDLLRPELKGKLAVTTESSTSRVVGTMVKYKGEEFVKRLRAQE
                     IRLFKASSAGFLDLIAAGEVAGSFVVFQNQVMVKKEKKAPVDWVPLDAVPTNAGGSAI
                     VANAPHPHAALLFTDFIIGAEGQKLMEQFRYGVAWKDYPFRREYPERGMTSTEYENAE
                     DKWLQLTRSISKK"
     gene            10817..12472
                     /locus_tag="VF182_09110"
     CDS             10817..12472
                     /locus_tag="VF182_09110"
                     /inference="COORDINATES: protein motif:HMM:NF006052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6437277.1"
                     /translation="MLGGHAVAQCVRNEGVRHLFCVPGESHIAVIDGLVNTPDIQLIT
                     NRQEGCAAFMAEGYAKSTRKVGVCLVTRGPGATNASIAIHSAKYDSAPLVLLVGQVGR
                     NARGREAGQELDYGQFFGSIAKWVVEINNPTHVPRVMTRAFHIARSGRPGPVVVSLPR
                     DVLEEKAEIPMVNPYPPAQPSPDPRLIDEMIGRIGAAKKPMLLVGSGVQYAGAWQELI
                     DFSDAFHVPVLTSYKRQDAFPNSHPNYIGNLSSANKNTRALASRDADLLVVLGCRLSQ
                     QTTGGFSFPRPGQAFIQIDADEQNIGQNARPELGIVADAKLALLAVLKQTAPQADPGR
                     ASWIAELHSAQKQFCTPKERPSRRVSMERVMADLRAALPRDAITTTDAGSFGQWAQRY
                     LEFEHPNSYVSPTLGCMGPGVPSAVAAKLAHPERLVVAHAGDGGFLMTGQEMATAKQF
                     GAKIITIVYNNDGYNSIRMHQEAQYPGRQYGTALENPDFAKLGEAYGALGLKVTRDEE
                     FMPALKKAMAADGSALIEVLTDYEYVTPTATLSELSGKTLQGE"
BASE COUNT         2863 a         3526 c         3573 g         2729 t
ORIGIN      
        1 tcgatcatct ctttgcagat ctttagcggc aagcctttgg tttgcggata atgagcgccc
       61 gtggaagcca ataccagttt gtgaacctta ttaggatgat cgagggcgag caactgcgcc
      121 accctgccgc ccatcgaatg acccacaacg atcgcgttct caaccttaag atgatccatc
      181 aaagccacga cgtcctcggc gaacatcgtc gtggtgtagt caatggaagg tttgctcgac
      241 tgaccggtgc cgcgatagtc gtgaatgatc acgcgatggt cgcgggagaa ctcctcgact
      301 tgatggatct tccaaacctc gccgtcgcac gcggtttcgc tcagaaatac catcgggcgg
      361 cccttgccgt gctcttcgta ataaatttcc acgtcgttca ctttgacggt tggcatcgct
      421 cctcctcgta gcccgctcgc tttccacctg actcgatgca tattcaaaga aagtttgctt
      481 ggcaagaacc ggcgcaaaat tccttgactg gagactcgcc atggcgatat tgcaaaaacc
      541 tgccggcaat ttcctggcaa ctcggaggct ccatgaaaga aatcgcgttt ccgtactcaa
      601 ttttgcggat ttgtgtgttt ctcttattgt gtgcacccgg tttggttctt gcgcagcaac
      661 cggccaaact cgcctttccc tacggtccgc tcgggctcaa cagcgtcccc tggatagtgg
      721 ctaaagaaat ccgcatcttc gagaagaacg gcttggaccc cgatatggtg tacgtcggcg
      781 cgtcggcggt gattgttcag tcgatgcttt ccggctcgtc taatgtcgca ggttttggcg
      841 gtcccgccgt catcaccaac gtcctgcgcg gcggtgatat catccaaatc gccgcgatgg
      901 cgccttactt tacccagtct ttgttcgttc gtcccgatat tcgcgatctg cgcagcctgg
      961 agggcaaaag aatcggcata acgcggttcg gctccgtcac cgatttcgcg cttagatcca
     1021 tcatcgagag gcacaacatc aagggtgtga acgttcttca aatgggcggc ttcccggaag
     1081 ccgtcgccgg actgtcgcgc ggagcaatcg acggcgcagt tttttcaccg ccgcacaatt
     1141 ttcgcatgct caaagaaggc tttcgcgagc tggtgtcgca gaaggatcta cgcgcgttcg
     1201 ggagcggatt tctcacccaa ggtattgtcg cgcgaagatc atttgccgca actcaccgcg
     1261 agattatcgt ccggctgatc aaatccacgg tcgagggcac taagtacgcg gtgactaatg
     1321 aagattcgac gaaacgaatc atcggtaaat atctcggcat tagcgacggc gaccttttgc
     1381 gccagagtta tctttatgtt gttgacactt tcgttcgtga gcccttcgtc cccgaaagcg
     1441 tgatgcaggc aatggtacag cgcatggttg aggtcaatat gatcgacgtg aaatccgccg
     1501 aagtgacgcc gatcagcgcc tacttcgaca atagttatgt cgcggagctg aaacaaagcg
     1561 gttttctcga cagtatttgg aagtaaattc tctcgttcgc ttgacgcctg atcgcgattt
     1621 cacgtagttt gcggatagta acgatgattg ccttacatct tcggagtaat caatgccgcg
     1681 ctggataaga gtaggttttt ccgttgccgt cccgcttgtt gttttcttcg tcgcaactgc
     1741 tggggcacaa gacagggtcc ggctgggtct cagttcgatc agcgcaacct ccggcagtat
     1801 atgggtcgcc gaagaaaaag ggctcttcaa gaagcatggc atcgacgcag aggtcatcat
     1861 tattggcggc ggcgcagcgc gcgtcgtcag ttcgctgatc gcgggcgaga ttcagttctc
     1921 cgtgggcggg ggcgacgcgg ttatccgagc ggccctgaga ggcgccgatg gcgtgctcgt
     1981 ggcttcgcca atgaagactg gcttgcagcg actgatggtt cggccggaga ttaaaacgcc
     2041 ggcggatctt aaaggcaaga ctatcgcggt cacccgcttt ggctcggcat cacattgggt
     2101 cctgcaactg tttctgcgca agtggggcat gcgccccgat gacatcaaga tgctccaact
     2161 cggctcgtca ccggcgatgt tcgcaagctt ggaaaaaggc gccgtagacg gcggcgcatt
     2221 tacgatcccg acgttctttc ttgctgaaga acgaggctac cggatcctgg ccgaccccgt
     2281 ggacatggag atctattatt tgcaaaactc catcgacagc acacgaggct acattaagcg
     2341 caaccgcgat cagtcgctgc gccttatcaa ggccctgtgc gagggtctcg cctattttcg
     2401 aaaaaacaaa aaggaaagca tggagatcat gcaaaaaaag ctgcgtatcc aatccgccca
     2461 ggaaagagac gtcaaatatc tcgagctttc ctataacctt ttagccgata agttttataa
     2521 ccgagtcccc tacgccacac ccaaagctgt cgagaccacc ctcgagttca ttgctggcga
     2581 ggagccgaaa gccaaaggcg ccgatccaaa acagttcata gacgagagtc tcgtgcgcga
     2641 agtcgaggcc agtggcttta tcaaaaaact ttacgacacc gactatcggt aatcattgac
     2701 atgaacttga tcatttcgtt gagtacgtca tcgctttatc cgatccatct gacggcttga
     2761 acgatttgaa cgctttgaac agaaggagcc cgaatggccg actacaaaga gattctttat
     2821 accaagcagc gcggcggagc gctgatcaca ttgaatcgcc ccgaggcgct caatgccatc
     2881 acccggccga tgttgaaaga gctccatcac gctctggatg aagccgaagc cgatcccgac
     2941 gtccgcgcta tcgtcatcac cggagccgga cgcgccttta gctccggttt cgatcaaacc
     3001 accaccacgg ggcgccgccg cgatatccaa tggccctacg gtattcaaac cggcatgagc
     3061 gccgctgatc tgatgaacta ctggagcgcc gatgacgtca agttcatccg catatttgaa
     3121 atgaccaagc cggtgatcgg cgcggtcaga ggttgggcca tggggggagg ctgctggcta
     3181 gcactttata cccacatcac catcgccgcg gaggatgcgg ttttcgccca gcccgaagtg
     3241 cggcacggtt ccaacacgac cttcatgtgg acgctgttgg cgggttttaa gaatgcccta
     3301 cgttatggac tggtcggaga tcatgtcgat gctcacgaag cgctgcgtat cggcctggta
     3361 aacaaagtcg tgccggtaga tcaactgctg gaggaaagct tcaagatcgt cgaacgcatc
     3421 tcccatgtgc cgccggaaac agtgaagatc aatttgcaga tttccaccat ggggttacag
     3481 atgatgggcc tgcgcgatgc gctgcgaatg gatgagactc tgtcgattcc agcccacatg
     3541 atgctcaacg aggagtttcg tcgtccgctc gacgaagctc gcgccaacca agggacaaag
     3601 gcttacctac agttgcgcga cggaccgttc cagccggaac cgttcggacc gcgatcgaaa
     3661 aaaaagacgg ggtaatggag cgtggagtaa agggtattgg ttttcccggc gcatcactcc
     3721 agtactccat cacttcaatt tggaactaga ctatgactca ttcgacgagt agcggcaccg
     3781 ccgtcgcctg gcgcgatctc tcgccgctca tgaatccgcg ttcggtggcg atcatcggcg
     3841 catcgcagcg tagcgcatcg gcgctcaatc gcgaacccag aggcaaccgg gtcatccgta
     3901 atctgaaaag cttcggttac gcaggtcgaa tcgtggcggt caatcccaag tacagcgagg
     3961 tgatggatta tccgtgctat ccggagatcg gcgcgatccc cgaacctgtc gattgcgtcg
     4021 tcttggctgt tcccaatcgc caggttcccg atctcttgga gtcggcggca attgcgggag
     4081 ttcgggccgc ggttgtcttc tccgccggct tcgccgagac cggggcagaa ggaaaacaac
     4141 gccaggctag actcgaagcg ctttcgcggg agcgcggctt tttgatctgc ggtccgaact
     4201 gctacggagt tttgaatgtg ttcgggaaag cgccgctctt cgcgtcgacc attccgcaag
     4261 gatttctcgg cggtccggtc gccctggttt cacagagcgg cggcctgagc acgaccattg
     4321 ccaacgcgtt aatgttgaat cgccatgttg gtctcagcca tatcgtctcg tgcggcaatc
     4381 aggcaggcgc taccgtcgag gaatatttta actatctggt cgaagacgaa aacacccgcg
     4441 tgatcgccgc ctttgtcgaa ggcttcaagc aaccgaatct attattggcg gtggcgcgca
     4501 aagccgccga acgaaacaag cctttaatta tcttgaaggg cggcagatcg gaggtttcgc
     4561 aacgggcggc ggccacccat tcaggatcac tcgccggagc ggcggaagtg atcgacgcgg
     4621 cgtttcgaca aagcggtgtc gtctcggttc gcagcatcaa tgaattgatc gatgccacca
     4681 gcgttttttc ctgcgacggc tttgttaaac actactgcgg tggcaggcgc ataggcgtgc
     4741 tgagcggctc cggcggcgaa tgcaccttgg tgtcggatgc agctgcgagc gtcggcctcg
     4801 aagtaccgga attaacgcaa tcgacaaaat ctcagctgca agaagcggtc gccgacttcg
     4861 gcaatatgaa taacccgctc gacggcaccg gcgccatgta cgacgatgaa aaaatttttc
     4921 cgcgtttact tcaggcgctg atcgatgatg ccaacatcga tgtcgtcacc atcaacctcg
     4981 aagccaatga tcccaggcca aaggaattaa agagcggcaa tcgatttgcc caggcaatcg
     5041 agagagccgc agcctccagt aaaaaaccca tcgcctgttt cagttcggtt gtcggtggtc
     5101 cggtggatcc cgagattctt ttgccgctac ggcaaggcgg cgtgccgctc atggaaggcg
     5161 cggagtgcgc aacaaccacc attcgtaatt tagctgaatt ttacgaattc caacaaaggc
     5221 gacaatcgga agcgactgga gaccagccgg ctccaggtcg aaaaatagct tccggagtgt
     5281 tgccggcgca agccgctttc caactgctgg aggaattcgg catctcaaca gttccgatga
     5341 ccctggcgca cagtccggaa gaagccgctg cggccgctga gaagatgggc ttcccggtag
     5401 cgctcaagat cgagtcagcg cagatcacgc acaagagcga tgtcggcgga gtggcgctga
     5461 agctctccga cacagctgca gtaagcgatg cctataataa aatcatgagc gaagtcaggc
     5521 gccgagctcc tgatgcaaaa atcgagggca tcgtggtgca acggatgtcg ggccaaggcg
     5581 tcgagatgat cctcgggatc aagcgcgatc cgctcttcgg cccggtcgtg ctctgcgggt
     5641 tcggtggcat tctcgttgaa cttcttaaag atgtcagcat cggcattccg ccgttgtcgc
     5701 gcaaacaggc gcatgacatg ctcaagcgcc tgcgtgggtt tccgattctc ggaggtgtgc
     5761 gcggccaagc ggctacggat atagatgcgt tatgtgatgc catcgccgga ctgtcgagac
     5821 tggcctggag cttcggtgat cagttggccg gtttggatat caatccgttg atcgtgctgc
     5881 caaagggcgg aggcgtcgtt gccgtcgacg cgctggtgga gattcgataa tgactcgaac
     5941 gatggcaatt gctctaagaa aatgatcgat tgttctcggc gtaggggcag gtttgaaacc
     6001 tgtccctaca aaatctccat tcctgcactg gcaggcttaa ttcaggcagc ggcgccattc
     6061 ccgccggagg gaaaatgaac aggagcttgc cgttaatcat aatcactgtc tgggccgttt
     6121 tattccttcc cggcttgact ctcgcgcaat caaagccgct caaagaggtt cgcgtgcctt
     6181 atgctctcgg cggctccacc ggattcttct gggttgcgca acgctccggc tcgtttgaaa
     6241 aatatgggct caaggttctg cccatcttca tgcgcggcgg ccgggaggca gtgcaggcgc
     6301 tcatctcacg cgatgtcacg atgctccagc aaggcagttc cggagtgatt ctagcgtggg
     6361 cacagggcgc taaagacctc gtggtgatcg gcgccaccgg caacaaactg gattacgtct
     6421 tagttagcag cgccgccatc aaaaagccgg ccgatctcaa aggaaagaaa atcgccatca
     6481 gccagttggg tgccggcacc gacttcatcg cgcgtcacgc cgtgcgccag ctcgggttga
     6541 acgagaagga tgtcactctc ctcagcatcg gcgcgcaggg ggaaagatgg gcggcgttgg
     6601 ctggcggcca cgtcgacgct tcggtgtttc aaccgccggt gaccctgcgc gcgcgcaaag
     6661 ccggcttacc gatctgggta gacttttcca aaaccgaata cgagtacgtc gtcgccggtc
     6721 cggtcacgac ccggtctttc atcaaaacgg atcgcgagac ggtgatgaat ttcatgcgcg
     6781 gtctggcgga tggtatggac ttctaccgcg acgagaaaaa taaggacgct gtgatcaagt
     6841 acctgggcga attctacaaa tccacgaaca ccgaagagtt ggaagagacg cggcaggtct
     6901 atcgacaagt caccccaggt ctgccaatcg ttaccttaaa atctatggaa aacatgatcg
     6961 ccagcgaccg catgttatcg accatgaaca tcaacggcgc cgatgtgatg gatctgtctt
     7021 tcttgagaca gctggaagag gaacggaaaa ccaaaaagta aacgttcaag cagttcaagt
     7081 cgttcaaatt gtcatccgga ttgttctgac ggcttgaacg ttttgaacta tttgaacgat
     7141 tttgaacctt tagacaggag ttccgatgcc cgactataaa gaaatacttt acgagaaacg
     7201 ccgcggcggc gccttgatca cgctcaaccg gccggaggcg atgaacgcga tcagccgcag
     7261 catgatcaaa gaaatccatc aagcgctcga cgatgtagaa gaagacaacg aggtccgcgc
     7321 cgtcgttctc accggcgccg gacgggcttt ttccgccggc atggaccagg gaaatcgcgc
     7381 cggccgtcgc cgcgatatcc attggcccta tggcattctc acgggacaga gcgccgccga
     7441 agtcatcgat tcctggcgtg tcgacccgcg caactttcgc cgcatgtggg aattcggcaa
     7501 gccgatcatc ggggcaatca atggatgggc aatgggcgca ggctcatggc tgtcgctgtt
     7561 cacgcacatt acgatcgcgg cggagaatgc cgtgttcgcg caacccgaag tgcgccacgg
     7621 ctccaacacc agttttatgt ggacgctctt gggcggctat aaaaacgcgc tgcgctacgg
     7681 attgaccggc gaccatatcg atgcgcagga ggcgcttcgt atcggcctgg tggtcaaagt
     7741 cgtgcccccc gaacagttga tcgatgaatg tttcaatctg gtcgaacgca ttgctcacgt
     7801 gccgccggaa acggtcaaga tcaatctcca ggtcgccacc atgggcctgc aaatgatggg
     7861 gctgcgcgac gccttgacat ttgacagcca aatgtcggcc cccgcgcata tcatgttgcg
     7921 cgaggaacta cgcaagccgc ttgacgaggc acggacgaac aaagggatcc gggagtatct
     7981 gcagatgcgc gacgggccgt tccaacccga accgttcggt cccaggtcga agaaaaaaac
     8041 ggagtaatgg attgttggag tgatgggcac cgacctccca gccaatactc cagcactccc
     8101 atctaaaaat ctaacaggag cgacagatga ctaattacaa caccattctc tatcagaagc
     8161 agcgcaaagg tgttctgatc acgctcaacc ggcccaatgc gctgaacgcg atgaaccagg
     8221 atcttatgaa cgagcttgat caggcgttgt ccgaagccga gatcgatccg gaaatcagag
     8281 cggtggtaat taccggcgcc gatggcgcat tttcggttgg tgaagacatc agcggcgatg
     8341 atccggaaac agcttggcct tacgggatac cggccggaac ctcgctgaat gccacgtaca
     8401 acaaattccg tgatggggat cgcaaggata ttctcggccg tcaagcgtat cgctggcaat
     8461 atcccaagcc gattatcggc gcggtaagcg gttggtgcct gggcgccggc tcctggctgg
     8521 cgctcacctg ccatatcacc attgccgcgg acgacgcggt gtttggccag ccgcaggtgc
     8581 ggcacggcgc caacaccgat tttatttggg tcgcgcttgc cggattcaag aacgcactgc
     8641 gctattcact gaccggcgat catgtcgatg ccaaggaggc tttgcgcatc ggattggtga
     8701 atcaagtagt cgccaaagat cagctgctgg agacctgttt tcaatttgtc gagcgcgtcg
     8761 cccacgtgcc gccggagacc gtgaaaatca atctgcacat ttcaaccttg ggcatggaga
     8821 tgatgggctt gcgcaaagcc tggacactca atgccgaact cgccgccatg gcgcgcctga
     8881 ccaagcgcga ggaattcaac aagcgcctcg aagacgcgaa gaaaaaaggc ggcctcgcgg
     8941 ccttcttgca ggaacgtgac gcgccgtttc agccggagcc gtttggccca cacgcaaaga
     9001 agtaaatctc gtttgaactc ttggctagtt tagcatggca taaagtaaat agcctatgtg
     9061 aataccgatg tacaaaatgc caccagctga agctacatcg ataagggcat cgagcatatg
     9121 tgtcagaaac ttcatacgtt tatcatcgtt gaaagtggga gagcggcaag gatacgctac
     9181 cgttgagagt aacgccgcgg atagaagctg taggctttgc ctccggcaga ccgagcgcgt
     9241 tgattgaggc ctcccgatat ttgatcgcgc agtgtatatc ggcacggagg atggcaagtc
     9301 aagcgcgtgg gaagtttacc tgccgttccc gttaagacgc cggcggcagt ggctacgcgt
     9361 tgctccatag tcgattggtt tacgttatgg tattgcccgc gcctctagtc ttgccgagca
     9421 ctgatcggca atcacttctt tgatatcgac cgggttagtt gcagccactt atcctccgca
     9481 ttctcatact cggtgctggt catgccgcgt tcgggatatt ctcttcggaa cggataatct
     9541 ttccaggcca cgccgtagcg aaactgctcc atcagcttct gtccttcagc gccgataata
     9601 aaatcagtga aaaggagcgc agcgtgcgga tgcggcgcgt tggcgacgat tgccgacccg
     9661 ccggcattgg tcggcacggc atcgagcgga acccaatcaa ccggcgcctt cttctctttt
     9721 ttgaccatca cttgattctg aaacacgaca aaagaccccg ccacttcccc ggccgcgatc
     9781 agatcgagaa atccggcgct ggaagctttg aacaatctga tttcctgcgc tctcaggcgt
     9841 ttgacgaatt cttcgccttt gtacttgacc atggtgccga caacccggct ggtcgagctt
     9901 tcggtggtta cggcaagctt tccttttagt tccggcctta gcagatcttg aaaattcttg
     9961 ggaatctccg ccggtgcgat ctgccgggtg ttgtagccga aacccaggta ggcttcgcga
    10021 tcggtcaccc agtagatacg cgggccatcg gctttggtct ttgcctcatc gggatatcgg
    10081 gccagctcgg gagatgtgta agctttgaga agacctctct cacgcagcaa catcaaaaga
    10141 cctggagtgc tttccaacgc gtcggccacg tgtcggccgg cttgcgcttc attgagcaag
    10201 cgcggtccaa ggtccgtgct gccgccgcga tagacttcaa cggcgacatc gggatacttt
    10261 cttttgaaag cgtctaccaa ttcccggtaa acaccggaca aagaggtgta ccagacgacc
    10321 ttaccttcgg ctttagcgcc gtcgagaaga atctgatggc gatccacgcc gttgtaggcg
    10381 gttagctcgt ccagcgtacg gggcttcttg gtttgccccc agccgagcga actcgtcgtt
    10441 acaaggatca gtaacgcaac gccaaaaatt ttcatgggcc attccccctt gactgtcgct
    10501 gccccgaagc cattacggtc ttgcccgagt tatcacgtct ccaggaagtg gagcaagcac
    10561 aaaccgcgca agcggaaaaa atgtagcgac cgtgtgtaca tgaaatcctg ctttcgtaga
    10621 aggaacaaac gccaatgctg ttattctaat gcgcaggcga agatcgttgt tgctactcgg
    10681 aaacagcatg gttgatgatg acgattcgac agcgaatgta attgacagcc aagatcgatc
    10741 cggactatag ggcaagtaat gattccagga tagagcggga tttcatctga gagaaatcgc
    10801 cactcgggag acaattatgc ttggcggcca cgcggtagcg cagtgtgtgc gtaatgaagg
    10861 agtgcggcat cttttctgtg tgccgggtga gagccacatc gcggtcatcg acgggttggt
    10921 caacacaccc gatattcagt taatcaccaa tcgccaggag ggctgcgctg ctttcatggc
    10981 cgagggttac gccaaaagca cgcgcaaggt tggcgtctgc ctggtcactc gcggtcccgg
    11041 cgcaaccaac gccagcatcg ccattcattc ggcgaaatac gattcggcgc cgctggtgct
    11101 tttggtcgga caagtcgggc gcaacgcgcg cggccgcgaa gccggccaag agctcgacta
    11161 cggtcaattc ttcggcagca tcgccaagtg ggtggttgag atcaacaacc cgacccacgt
    11221 gccgcgggtg atgacccgcg cctttcacat cgcgcgcagc ggccgtcccg gcccggttgt
    11281 cgtgtctctg ccgcgcgacg tgttggaaga gaaggccgaa ataccgatgg tgaatcctta
    11341 tccgccggcc caaccgagtc ctgatccgcg cttgatcgac gaaatgatcg gccgcatcgg
    11401 agcggctaaa aaacccatgc tgctggtcgg ctcgggcgtc caatatgccg gggcttggca
    11461 ggagctaatc gatttttctg acgcgtttca cgtcccggtc ttgacctcct acaagcgcca
    11521 ggacgctttt cccaacagcc atccgaatta catcggcaat ctatcgagcg ccaacaagaa
    11581 cacgcgcgcg ctggcgtcca gggatgccga cttgctggtt gtgctcggtt gtcgtttgag
    11641 ccaacaaacc accggcggct tttcttttcc caggccgggc caagctttca ttcagatcga
    11701 cgccgacgag cagaacatcg gccaaaacgc gcgcccggaa ttgggcatcg tcgccgatgc
    11761 caagctcgcc ttgttggcgg tgctgaagca gaccgcgccg caagccgatc ccggccgcgc
    11821 ttcctggatc gccgaactgc acagcgcgca aaaacagttc tgcacaccga aggagagacc
    11881 gagccgccgg gtttcgatgg agcgggtgat ggctgacttg cgagctgcgc tgccgcgcga
    11941 cgcgatcacg acaaccgatg ccggcagttt cggccagtgg gcgcagcgct atctcgaatt
    12001 cgagcatcca aattcctatg tttcgccgac actcggctgc atgggcccag gagtgccatc
    12061 ggcagtggcc gccaagctcg cgcacccgga gcgtttagtc gtcgcccatg ccggcgacgg
    12121 cggtttcctg atgaccggtc aggaaatggc aaccgcaaaa caattcggcg cgaaaatcat
    12181 cactatcgtc tataacaacg acggctacaa ttcgatccgc atgcaccagg aagcgcagta
    12241 tccagggaga caatacggca cggcgttgga aaatcccgat tttgccaaat tgggcgaggc
    12301 ctacggcgcg ctgggcttga aagtcacgcg cgacgaagag ttcatgccgg ccttaaaaaa
    12361 agcgatggcg gctgatggtt ctgcgttgat tgaagttttg accgattacg aatacgtcac
    12421 ccccaccgca acgctgtcgg aattgtccgg taagacactg cagggagaat agctactcat
    12481 cctgatcgga ctcgttgcag atgtcgcccg tcgtaattga tggtccgcaa gtttgccaaa
    12541 aacttaacgt ggttcgatat agccggtccg tgtctctaac aaaacgcgtt aacagcaagc
    12601 gcgaatatcc catccgaatc atcggcggaa acggagacga gaaaaatgaa ggttatgagc
    12661 ttcttaatct tggctctttt atttctatca a
//
LOCUS       DASUVS010000163         4563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1594534, whole genome shotgun sequence.
ACCESSION   DASUVS010000163 DASUVS010000000
VERSION     DASUVS010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594534"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VF182_09115"
     CDS             complement(<1..1302)
                     /locus_tag="VF182_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6437278.1"
                     /translation="MIGARSASIFPAENAIPEKWRIASPMEQSHYLCDGEIKEWTGPQ
                     QDVLSPICEVINNNLQQKLIGSYPLLAEQQALEALNAACRAYDDGRGTWPTMSVEERI
                     RHVDAFAYRMKEKRDDVVRLLMWEIGKTLPEAEKEFDRTVDYIRDTIDALKDLDRVSS
                     RFVIEQGVIGQIRRAPLGVVLCMGPFNYPLNETFTTLIPALIMGNTVIFKPPKLGVLL
                     HEPLLEAFRDCFPKGVVNTIYGEGQKVIGPLITSGKIDVLAFIGSSRVADILKNQHPK
                     PHRLRCILGLEAKNAAIVLKDADLDQAVKECVLGALSYNGQRCTALKMLFVHREIVDA
                     FIANFSNAVDRLKAGMPWEDGVCITPLPEPDKTRYLTELIDDARAKGASVVNRGGGSV
                     EHTFMSPAIVYPVDPTMRLYSEEQFGPVVPITPFSDVGEAMH"
     gene            1585..2739
                     /locus_tag="VF182_09120"
     CDS             1585..2739
                     /locus_tag="VF182_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX6437279.1"
                     /translation="MSCIASFAIFAVDLPFRQLFKHAAAERGSSDSLFIKCATEDGHI
                     GYGECLPREYVTGETRDGTFEALRDRLLPRLLGMKFTSLEEVKGFLVECDGKAPKEWG
                     STNEPQTAAWCAVDLALLDCFGHALQEPVRLGPAAAFPGSVRYSPVVSAESGFKFLKT
                     LIKLRLYGFRQVKLKLGGGNDTRLAALTRRVLGRRVDMRADANMAWSADEALTIMRNL
                     SKSGVRFFEQPIASGNINGLARLVKESGLEVMVDEGLSDRASLRKLINERACTAVNVR
                     ISKCGGLMASLARCREALDAGLAVQIGCQVGESSLLSAAHLVLVMAVQKVLYAEGCFG
                     YHLLREDPAEPVLQFGYGGRAPALPNGAGLGITINEDILRRWSVQEAIVG"
     gene            2768..4444
                     /locus_tag="VF182_09125"
     CDS             2768..4444
                     /locus_tag="VF182_09125"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEX6437280.1"
                     /translation="MSLLLDVLFSFLGSRTLATFQDECRKPTETQERLLNRIITKNRE
                     TAFGTAHRFPNIASFAHFQRRVPITTYEELKSYIDASLRGEPNQLTAERPVLFATTSG
                     TTGASKYIPVTPDSRPLKSYLMRVWLSALHRDHKIFGGQILTIVSPEVESYAPCGTPC
                     GAESGHGYKNIPRTIRSLYSCPYEVYEIKDYEAKYYCLLRIAAGQSISLIYTCNPSTV
                     LLLGQRLAQFTEPIIRDVRDGTLSREFDIPGDLRAAIEGQLSANPGRAAVLEKAASES
                     AGDLLPRHVWPELAAIGCWKGGTVGMYVEKFDQYYPPGLAVRDIGYFASEHRGSVPMT
                     DVGSSGVLAIPTNVYEFFPLEEDRKPKGAELLTVEELEQGQRYYIYVTTAAGLYRYEM
                     NDIIEVTGFYEKTPLIRFIQKGKGVVSFTGEKLYESQVLASVDDAFAPLLGRYEFIAA
                     IGEMRGAKPCYVFLIEFDQPVQDRPAKLMLNSLERALRSHNTEYASKRDSMRLDPPVL
                     RIIKSGEFDRYRKRAVEKGKSDGQFKILRLITDDSFAREFDAEREVTMNP"
BASE COUNT         1045 a         1223 c         1214 g         1081 t
ORIGIN      
        1 gtgcatcgcc tcaccaacgt cggagaaggg agtaatcgga acaacaggcc cgaactgctc
       61 ttcagaatac aacctcatgg taggatcaac gggatacacg attgcaggcg acatgaaagt
      121 atgttcaacg gaacctccac cgcgattaac cacgctagca cccttagcgc gagcgtcatc
      181 gatcagttcc gtgaggtacc gggtcttatc gggttccggt aatggcgtga tgcagacccc
      241 gtcttcccaa ggcatccccg ccttgagtct atctacagcg ttactgaagt tagcgatgaa
      301 ggcatcgaca atctctctgt gaacaaacag catcttgagc gccgtgcacc gttgaccatt
      361 gtaagagagc gcccccagaa cgcattcctt tacagcctga tccaagtccg cgtccttcaa
      421 tacgattgct gcattcttgg cttcaagtcc gagtatacaa cgcaggcgat gcggtttcgg
      481 gtgctgattc ttgagaatgt cagcaactcg actgctgcca atgaatgcca gtacatcgat
      541 ttttccggac gtgatcagcg gtccaataac cttctgcccc tctccataaa tcgtgttgac
      601 gacaccctta gggaagcaat ctcggaatgc ctcaagaagt ggctcgtgga gcagcacccc
      661 caatttagga ggcttaaaaa ttactgtgtt gcccatgatc agtgcgggga tcagggtggt
      721 gaaagtctca ttgagcgggt agttgaacgg ccccatgcaa agtactacgc caagcggagc
      781 gcggcgaatc tggccaatca ccccctgttc aataacaaaa cgtgacgata cgcgatcgag
      841 atctttcagc gcatctatag tgtcccttat gtaatcgacc gtccgatcga actccttttc
      901 ggcttcggga agggtcttcc ctatctccca catcaggagt ctgacgacat catcgcgctt
      961 ctctttcatc ctgtaggcaa atgcgtctac atgcctgatc cgttcctcga cagacatggt
     1021 cggccatgtc cctcgtccgt catcataagc ccggcaggct gcattaagag cttccaaagc
     1081 ttgctgctcc gcgagcaagg ggtaactccc gatgagcttc tgctgaaggt tattgttgat
     1141 tacttcacat atcggcgaca gcacatcttg ctgcgggccg gtccattctt tgatttctcc
     1201 gtcgcagaga tagtgacttt gctccatcgg tgaggcgatg cgccacttct ccggtattgc
     1261 attctcggca ggaaaaatcg aagcggatcg cgcgcctatc atttgcatgc tcctttttat
     1321 tcaacatgcc ggttgatgat cgggttcttt cagctttgcc tggtccttgc ttcgatgacg
     1381 cttggacacc agagttccct cgatcggctg atccatgtgg ttgagagaat taatagcgaa
     1441 cagttgaacg gctcggtatt ggaccttggt ccaatagtcg agagcagatg cttcgaatag
     1501 taatcaaagt catcgaggct aaaaaccggc ctagtattgc gtcacgaaca aagacaaaca
     1561 attcggagaa accaagtcgc ggcaatgtct tgtatagcaa gttttgcgat atttgcggtc
     1621 gatctaccgt ttcgccaact attcaaacac gccgccgcag agcggggttc gtccgatagt
     1681 ttgtttatta aatgcgccac agaggacgga catataggct acggcgagtg tctgcctcgc
     1741 gaatacgtga cgggcgaaac cagagatggg acgtttgagg ctttgcggga taggctcttg
     1801 ccgcggttgc ttggtatgaa gtttacttct ctggaggaag tcaaagggtt tctcgtcgaa
     1861 tgcgacggta aggcgcccaa agagtggggc agcactaacg agccacaaac ggcagcttgg
     1921 tgtgccgtcg atctcgcgct gcttgactgc ttcgggcatg ctctccagga gccggttcgg
     1981 ctcggaccgg cggccgcatt tcccggaagt gtaagataca gcccggtcgt atccgccgag
     2041 tccggcttta aatttttgaa gactctgatc aaacttcgtc tttacggatt tcgccaagta
     2101 aaattgaaac ttggaggcgg gaacgatact cgcctcgccg ctctgaccag acgcgtcctt
     2161 ggaaggcggg tcgatatgcg cgccgacgcg aatatggcgt ggagcgcaga cgaggcgctg
     2221 actatcatgc gcaatctctc caagtctggc gtacgctttt ttgagcaacc catcgcgtca
     2281 ggaaatatca acggacttgc ccgcttggtg aaggaatctg ggttggaagt tatggtggac
     2341 gagggcctaa gcgatcgtgc ttccctgcgg aagttgatca atgaacgtgc ctgcacagca
     2401 gtcaatgtga gaatctcaaa gtgcggcgga ctcatggcgt cactggctcg ctgtcgcgag
     2461 gcattggacg ctggcttagc cgtgcagatc ggatgccagg taggggaatc ttcgttgctg
     2521 tcggctgccc acctggtact cgtcatggct gtgcaaaagg tcctgtatgc ggaaggttgc
     2581 tttggctacc atctgctacg cgaggatcct gcagagccag ttctccagtt tggctacggc
     2641 gggcgcgccc cggcactacc gaacggtgcc ggcctgggca tcacgattaa tgaggatatc
     2701 ctcagacgtt ggagcgttca ggaggcaatt gtcggatagc ctaaaacaga gcaggagcga
     2761 ttgagccatg tccttgcttt tagacgtcct attttctttt ctgggatcgc ggacactagc
     2821 aactttccag gatgaatgcc gcaaaccgac cgaaacacaa gaacgactgc tgaatcggat
     2881 cattactaag aatcgggaaa cggcgttcgg caccgcccat cgctttccca atattgccag
     2941 cttcgcacat tttcagaggc gcgtacccat aacgacctat gaagagttga agtcttacat
     3001 cgacgcgagt ttacgcggcg agccaaacca attgacagcc gaacgaccgg tcctttttgc
     3061 gaccaccagc ggcacgaccg gtgcatcaaa atacatcccc gttactcccg atagtcgtcc
     3121 cttaaaatcg tatctaatgc gagtttggtt atccgcactg caccgggacc ataaaatatt
     3181 tggcggacag atcctaacca tagtgagtcc ggaagtggag tcctatgctc cttgcgggac
     3241 accttgtggc gccgagtccg gccatgggta caagaatatc ccccgcacca tccggtcact
     3301 ttattcgtgt ccgtatgagg tttacgagat caaagactac gaagctaaat actactgtct
     3361 gctgcgcatc gctgctgggc aatccatcag cctcatctac acctgcaatc cgagtacggt
     3421 ccttttgctc ggccagcgcc tcgcccagtt taccgagcca atcatacgtg acgttcgcga
     3481 tggaacgctt tcgcgggagt ttgacatacc cggcgatctt cgtgccgcaa tcgaaggaca
     3541 attgagcgct aatcctgggc gcgcggctgt gcttgaaaag gccgcttcgg aaagcgccgg
     3601 tgatctcttg ccgcggcatg tctggccaga gctggccgcc atcggttgtt ggaaaggtgg
     3661 aaccgttggt atgtatgtcg agaaatttga tcagtactat cctccgggtc tggcggttcg
     3721 tgatatcggc tattttgcca gtgaacaccg cggctcggtt cccatgactg atgtaggttc
     3781 ttccggtgtg ctggccattc ccacaaatgt ctacgagttt ttcccactgg aggaagaccg
     3841 aaagccgaag ggagccgagc tcctgaccgt ggaggagctc gaacaaggtc agcgctacta
     3901 catttatgtg acaaccgctg ctggccttta tcgatacgag atgaacgaca tcatcgaagt
     3961 cacaggtttt tacgaaaaga caccgctcat ccgttttatc caaaagggca aaggagtcgt
     4021 ctcctttacc ggcgagaaac tctatgagtc acaggttctc gcatcggttg acgatgcctt
     4081 cgctcctttg ctgggacgct acgagttcat tgctgcgatc ggtgaaatgc gtggcgctaa
     4141 accctgctac gtcttcctga tcgaatttga ccaaccggta caggaccgac cggcaaagct
     4201 catgctcaat tccctcgaac gggcgctccg ctctcataat acggaatacg cgtccaagcg
     4261 ggattcaatg cggctcgatc ctccagtgct tcgcatcatc aagtccggcg agttcgatcg
     4321 ctaccgaaag cgcgccgtcg agaaggggaa gtccgatggg cagtttaaga tcctgcgatt
     4381 gatcaccgat gacagctttg ccagggaatt cgacgccgaa cgagaggtga ctatgaatcc
     4441 ttaggaggac actttatggt gagaacgcgt gagatactca aatcgcatcc ccttttgaac
     4501 agcttcagtg atgacattat cgaccgtttc ttgaactcgg ccgacacccg caggtactcg
     4561 gct
//
LOCUS       DASUVS010000164        25581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1375984, whole genome shotgun sequence.
ACCESSION   DASUVS010000164 DASUVS010000000
VERSION     DASUVS010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 25581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25581
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375984"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VF182_09130"
     CDS             <1..406
                     /locus_tag="VF182_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX6437281.1"
                     /translation="NLATPDGKKISLRDFRGKIVMLNFWATWCVPCREEMPAMEKLYQ
                     EFKDKNFVILAVNVKDRKPDAVNFAKELKLSYPIVIDADAQVGLLYGAWGLPTTYLIG
                     PKGEGLARAWGPAEWYSPAARNLIKDLLDGKR"
     gene            403..1140
                     /locus_tag="VF182_09135"
     CDS             403..1140
                     /locus_tag="VF182_09135"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX6437282.1"
                     /translation="MSDVNVFVAFAAGIFSFLSPCVLPLIPSYISFISGVSLEDMRHD
                     KTLNRVRARVVLNSIAFIAGFSLVFISLGVSASFLGSLFFGYRNTIRIVGGIFILLVG
                     LYLIGIFKIPALERYLQFNLKDKPAGYLGSVLVGITFAVAWTPCVGPILGAILALAGT
                     SGEIGRGTLLLSSYAAGLGLPFFLSAVAINSFMQFSQSFRRYIRAFHIAGGILLVIVG
                     LLLITDYMTMLNIYALRFTPDWLLKKL"
     gene            1256..1822
                     /gene="folE"
                     /locus_tag="VF182_09140"
     CDS             1256..1822
                     /gene="folE"
                     /locus_tag="VF182_09140"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX6437283.1"
                     /translation="METDNSMEEQIRLILKSLGEDPEREGLVKTPQRVAQALTFLTRG
                     YDMDPAKVINDALFTEDYQEMIVQKDIDFFSLCEHHILPFFGKAHVAYIPHHKIVGIS
                     KLARLVDVYARRLQVQERLTNQIATIIMEKLDPLGVAVVIEAEHLCMRMRGVEKQNSM
                     VITSTLLGAFQNRQETRNEFMTLIRQRG"
     gene            1833..3311
                     /locus_tag="VF182_09145"
     CDS             1833..3311
                     /locus_tag="VF182_09145"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX6437284.1"
                     /translation="MEIRFKDIGPENLKTDLLVVPVSERRLEDTSIRVLDRRLKGRLQ
                     ERIQKSKFAGAEGSILLYSTAGALPAPHLLLIGLGKAADIDAEAWRRAGARARREASA
                     LGIEEIGFFFSPERDPADAAAAIVEGTLLASYQFTKYRSNARNPLAIKTLTLHKSGLS
                     RSSAMDKAVRTAEQTVGGVFLARDLINEPPSVTTATYLGEQAEHHCRGRGLSVDVWGK
                     RKIESMKLAGLLAVNRGSQEEPRFITIHYTPAGKPRKKVALIGKGITFDSGGLSLKPS
                     KSMETMKLDMAGGAAVIAAMSRLPALRPEVEVIGYIPTTDNLPGPNAQKPGDVIRYLN
                     GKTVEVLNTDAEGRLILADALALAVKQKPDYMINLATLTGACMVALGTQVGGIFSNHP
                     ALADRILRCAQQAGEKLWQMPLVKEYREDIKSSVADIKNIGGSHGGAIIAALILQEFV
                     EEIPWAHLDIAGPAYTDRDIGTCPKGGTGFGVRTLLKFLMTI"
     gene            3328..3660
                     /gene="trxA"
                     /locus_tag="VF182_09150"
     CDS             3328..3660
                     /gene="trxA"
                     /locus_tag="VF182_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015113487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX6437285.1"
                     /translation="MGKITEVGDNNFEAEVIQSNVPVLVDFWAPWCGPCRSIAPIVEE
                     IAGEYEGKLKVTKLNVDDHPATASRYGIRGIPNLLILKGGAVKEQIVGAVPKAKLVSA
                     IDKALQQQ"
     gene            complement(3833..4936)
                     /gene="rodA"
                     /locus_tag="VF182_09155"
     CDS             complement(3833..4936)
                     /gene="rodA"
                     /locus_tag="VF182_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX6437286.1"
                     /translation="MQIDRRLISHFDWPLFLLTLAFVGMGIVTIYSANYDIAANHAGG
                     LPSRQLLWLGLGLAVMFTTIAFDYHYVDRLAYPFYGLMLVLLILVFFVGHAGGGSQRW
                     INLGFFRLQPSEPAKLAIVLVMAKYFQDSEPSRGYCLRDLWAPFALVAPLIGLTLIQP
                     DLGTAIILGLVFMSMMLIGGLRIKSFLGLAGVGLGFLPIGWNLLWPYQRARVLTFFDP
                     DRDPLGAGYHVIQSQIAIGSGRMFGKGYLHGTQNRLDFLPAQHTDFIFAVFSEEWGFA
                     GCFVLLACYFAMMLYCLKLFERAKDRFGALLTFGMVAILFWHVVINVSMVSGLMPVVG
                     VPLPLFSYGGSALASMMFAIGMMINVSMRRYTF"
     gene            complement(4926..6803)
                     /gene="mrdA"
                     /locus_tag="VF182_09160"
     CDS             complement(4926..6803)
                     /gene="mrdA"
                     /locus_tag="VF182_09160"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX6437287.1"
                     /translation="MAILGDVARKDGAPELRVRTRMVYLILIIAFLGIMSRLAVLQIA
                     HGERYTYLSENNRVRIKRVPGTRGMIMDRQGELMVDSRPSFDLLFVPEDAQDPQNTLR
                     QLASYLGRPENELIAVFEENKSRAAFDEIVLGRDVDWATVVAVETHQLDLPGVNLRVR
                     PRRNYADGPIAAHVLGYLGEIGPKQLKLLKEQGYGSGDEIGQYGLEKRWEGFLKGQNG
                     GQQVEVDALGRRVRVLHEVVDVPGYTVYLTLDRQLQQTAYEALKGKEGTIVALDVHTG
                     AILTMVSTPAFDPNIFARGIKSEEWRALIKDRLRPLNNRAIQGQYPPGSTFKIIMAIA
                     GLEEGIDPDARISDPGFFPFGNRSFRDWKKGGHGSVDLHKAIVESCDVYFYQLGQRLG
                     IDKIAHYARAFGLGEKTGAFLDDEKDGLVPDTAWKRKRYRQPWYPGETPSVAIGQGYL
                     TTTPLQLANMMAAVANGGTLYRPRIVNKVESVDGTMAREYGPEKIRTIDLKPATLHRI
                     QAALADVVRGKGGTGGAARSSVVSVAGKTGTAQVVEMKAAYLKSEQLSYFNRDHAWFV
                     AYAPIENPQIAIAVLIEHGGHGGEAAAPMAKKVFEKYIEMKNQPKDQQQARAPGEIGA
                     D"
     gene            complement(6803..7306)
                     /gene="mreD"
                     /locus_tag="VF182_09165"
     CDS             complement(6803..7306)
                     /gene="mreD"
                     /locus_tag="VF182_09165"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX6437288.1"
                     /translation="MKLSLLLFAVGLLLVLLQTTFLHFFPLVPDLILVLCVYWGLHHP
                     SVGAVLASFVLGYSVDVVSSPVLGINALAMSLVFLSVYLSSRSIWLHNHLVSAIVVLL
                     AALVKGVALALVSAIFLSSEGFWIAALRYILLEALVAALLAPFVFSLLRRGQTYLEKV
                     QVPGLYY"
     gene            complement(7327..8160)
                     /gene="mreC"
                     /locus_tag="VF182_09170"
     CDS             complement(7327..8160)
                     /gene="mreC"
                     /locus_tag="VF182_09170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX6437289.1"
                     /translation="MLAFFRKYQIPLSCCFCIVLSLYILSASARGQLKNDPIGPLLLW
                     LIRPLQIAAYSTTSWIKGLQESHLTAAGYKAENERLRERILELEMERNRFLEAEATNQ
                     QLRHLLDFRSRLPAASITASIIASSASNWFQSCTIDKGTADGVRKGMSVVTPLGVVGR
                     VVDAAARSAKVLLLTDPNSGIDVMVQRTRARGIVSGSLENGTVLKYIKRTEDIQVGDR
                     LITSGLDGVFLKGWIVGTITKVRKETLGLFQVIEVMPAVNPARTETVLVVSAEGKSAT
                     K"
     gene            complement(8209..9243)
                     /locus_tag="VF182_09175"
     CDS             complement(8209..9243)
                     /locus_tag="VF182_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX6437290.1"
                     /translation="MLDALFGVFSNDLAIDLGTANTLIYVKNQGIVCNEPSVVAVQQR
                     NERGGKKVLAVGAEAKRMLGRTPGSIVAIRPLKDGVIADFEITEAMLRYFIQKVHNRR
                     TLVRPRIIVGVPFGITEVEKRAVRESAESAGAREVYLIEEPMAAAIGSGLPITEPTGN
                     MIVDIGGGTTEVAVISLSGIVFSRSVRVGGDKMDEAIAQFIKRKYNLLVGERTAELIK
                     ITIGSAYPGDEIQTMEIKGRDLVAGVPKTVVITDEEIRDSLLEPINQIVEAVRLSLER
                     TPPELASDIVDRGIVLAGGGALLRNLDILLREETGLPVMMADDPLTAVVMGAGKALDE
                     ISLLREVAVH"
     gene            9447..11357
                     /locus_tag="VF182_09180"
     CDS             9447..11357
                     /locus_tag="VF182_09180"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX6437291.1"
                     /translation="MLDFIRKKKRSWIITILLGLIIVVFVAFYGGSQQPTGVAAHVAE
                     VNGESISQREFILHYQRAVERYREMFKGSLTPELLKNLNIKNSLLEEMIETRLVLQEA
                     RRLGLTATDEELMNAITQVPEFQVNGRFNKERYIQLLRANRLTPAQFEDDQRKQLTIQ
                     RLLGILADSAHVTEAGVRERYRFEQEKINLQFIRFSASENLSEVNISDDDIQKFYDRN
                     KESLKEPLKVQVEYIPYPFDQFSTPVQLTDKEVEDYYNANRPTRFTTPKQAKVRYIMV
                     TVDPAADAKQKEAARVRADRIVAEARAGKNFAELAKKESGDPSKEKGGEIGWLNQGQL
                     PEALDKQIFALAKGEISEPIETPVGFHIVKVEDIKEARTQSLAEATPTITRELKLEKG
                     KYEAAKIADRDREKAASGNDLAKVAQESSLLVKTTRLFSEGEVLPEIGPTQNFYKTAL
                     SLGATEVSSVIEGPNAYYIMKTKERKEAFVPALEAVRGKIEKGLKESKAYEMAVQKAN
                     DVLDQLKKEPNLAKLARDKKLKLEETGWFARNTQQLPKVGELQNLTVGSLALSASKPI
                     PDKIFTQADAAFVFAFKEAQAADMAQYEKDKAQLMQQALAETRQRILLKFKDELKAKA
                     KIEVNSGPLEEI"
     gene            11344..11991
                     /locus_tag="VF182_09185"
     CDS             11344..11991
                     /locus_tag="VF182_09185"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6437292.1"
                     /translation="MKKSDCAFVLMQIVLLRHGATDWNLQGRCQGSSDRQLTEVGLRQ
                     AEQIATHLSNETIHAIYSSSLERARQTAQLVSRPHDLPVMIEDDVRELDHGELEGLTF
                     NEIKEKFSDFLTGWRTAPAELQVPGGERLADVAERAWSAVTRIAQRHEPDHTVIVVSH
                     NFPILGIVCRLTRTDLNNYRLFHLDPCGITRITQNGNSRWEVTHVNSKPYSLPRP"
     gene            complement(12014..13573)
                     /locus_tag="VF182_09190"
     CDS             complement(12014..13573)
                     /locus_tag="VF182_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX6437293.1"
                     /translation="MPQRPFNPPWFVRKDLDGFFGLMIDNLIQLILIVSLCRELIHLP
                     DDYIFGKILPGAAFSILVGNLFYAWQARRLARDTGRDDVTALPYGINTVSLFAFIFFI
                     MMPIYQETQDPVWAWKVGLVACFLNGVIEIAGAFVADRVRGVTPRAALLSALAGIAIT
                     FISMDFTFRIFAQPLVALIPLALIFIAYFSQQKFPLGLPGGMVAVAVGTLLGWWLGAL
                     KGPPPAVDFALARPDFTGGSLLEALRHPAFLNYLSVIIPMGIFNVVGSLQNIESAEAA
                     GDRYRALPSLLANGFGSVVAASLGSVFPTTIYIGHPGWKRLGARSGYSVLNGLFIALL
                     CLTGTIQGVLGFIPLEAGIGILLYIGVIIVAQSFQETPKEHAPAVALGIVPALAAWGL
                     LMVETGLRVAGKSLHSVGLAPFGQNPAMHGMISLQQGFIFTSMILAAIGVALIEKQFK
                     KAAGWSLAAAVFSAIGLIHAYDLTPAGVAARFGLFEAPEFAASYLMLALLFLAFAFAP
                     DKQKKNTNVDC"
     gene            13676..14398
                     /gene="lipB"
                     /locus_tag="VF182_09195"
     CDS             13676..14398
                     /gene="lipB"
                     /locus_tag="VF182_09195"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX6437294.1"
                     /translation="MDKTKPLMAQLLYQDLGRLDFAAALSRQEQLLELKQREAIPDIL
                     LCVEHPHVYTIGRGGDLNHVIAARDVPLYRTSRGGDVTYHGPGQLVVYPIIDLRSKLR
                     RDVHRYVRNLEMTAIETLKDFGIEAARHPPFTGIWCDNRKIAAIGIAVRRCITLHGLG
                     LNVNTDLSYFNRIVPCGLAWAEVTSMAKELDKELSARNVRESFLRHFADLFGYTEIEI
                     ISHFSEFQAGQKGASYEARDDR"
     gene            14486..15334
                     /gene="lipA"
                     /locus_tag="VF182_09200"
     CDS             14486..15334
                     /gene="lipA"
                     /locus_tag="VF182_09200"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX6437295.1"
                     /translation="MPRRHPEWIKVKSPGSPNYLRLKRILREKNLHTVCEEARCPNIG
                     ECWGNRTATFLILGDTCTRGCRFCAIDKGKPLALDPEEPRNVARVVKDLGLDHIVVTS
                     VNRDDLPDGGSSHFAKTVFWIKSLSPGIRVELLIPDFDGNLSAVKTVVDSGIEILNHN
                     IETVPRLYGTVRPGHSYECSLNILKSAKEMRPDVLTKSGLMLGVGEGYEEVIGTLRDL
                     RRNEVDIVTLGQYLQPSNRQLKVDRYVTPDEFSEFKVAAEKLGFRHVESGPLVRSSYH
                     AWSHVN"
     gene            complement(15511..16857)
                     /locus_tag="VF182_09205"
     CDS             complement(15511..16857)
                     /locus_tag="VF182_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX6437296.1"
                     /translation="MELTNLHLLSATEAARLIRDGIINSEQLVEGCLARIREVDPQVQ
                     AWTFLDEDYALAQARALDERRLSGGAIGPLHGVPVGIKDIFDTADMPTECGSVLYRGR
                     TPLRDATVTALLRAAGAVIMGKTVTTEFAYFSPGKTRNPHNPEHTPGGSSSGSAAAVA
                     ANMVPLALGSQTNGSIIRPAAYCGVIGFKPTHGLISRHGALTLSRTLDHVGVFANSVA
                     DIALFAEHIAGYDQNDADTRPRARLPLAEIAAEEPPLAPMFAFIKTPNWQSADEDTRE
                     GFAEIIEHLGGQVEEVELFPSAVNAWQWHQSIMGAEMAYNLERPWKNSGSRLSEQLRA
                     QIERGREVRAADYLSALGRINLVHDSFLELFEQRYDAILTPAAPSAAPKGLANTGDPL
                     FCTLWTLCGMPALSLPLLQSSTGMPLGVQLVGPRYGDARLLRTARWLAQHIAAEKS"
     gene            complement(17121..19196)
                     /locus_tag="VF182_09210"
     CDS             complement(17121..19196)
                     /locus_tag="VF182_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX6437297.1"
                     /translation="MSDPALGLTMLGLIVIAIMMGFPTAFTLMGLGMLFGFLAFFDPA
                     AHWWDNRIFDLMVQRTYGVMVNDTLLSVPLFVFMGYIMERAALVDRMFHSVQLAFRRV
                     PASLAVTTLLVCAFWGIASGIVGAVVVLMGVIAMRPMLNAGYDVRLASGAITAGGTLG
                     ILIPPSVMLIVYAAVAGQSIVKLYAAAMLPGFFLTFLYLVYILGWAMINPKIAPQLSS
                     DQYRITPPQWLQLLSGGGTKRIATKLLAAVLQPHLLHGATTIAGKPVGYSFLVKNILV
                     LLVPLTLTIGVFLTTWWYVTIYNAAPAASVAPSVEMQSVPHESTASPVENKPQELGQA
                     AETLQELGSAGEEPGSAETTTMPSEPAPLAEAPPEEMGSLAESPGVPASGRVPEHFYL
                     WFWSLAAVSTLLLSIYYYRMDGEGFEILKQLVISVVPLGVLTVVVLVVILFGICTATE
                     SAAIGALGALYLAVMVKYPRVCAWSSMLGAIAGFILGLGRGDVATLVVASSLGATFIG
                     TAVPLFIYLPKSLELRTNIKQSVFLTAKTTAMVCWLFIGSALFSSVFALHGGQGLIER
                     WVLSMNLSPVGFLIISQMIIFLLGWPLEWTEIIVIFCPIFIPLLGHFQVDPILFGTMV
                     AVNLQAAFLSPPVAMSAFYLKGVSPPHVTLNQIFAGMMPYMLIVVLCLICMYIWPGMT
                     LWLPEFLYG"
     gene            complement(19193..19867)
                     /locus_tag="VF182_09215"
     CDS             complement(19193..19867)
                     /locus_tag="VF182_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018240174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX6437298.1"
                     /translation="MNVQALIRGIDQVSYWTGKAFAWLIMALTLVVSVEVFKRYILNA
                     PTAWIFDFNSMLYGTLFMMCGAYTLALAGHVRADFVYIYLRPRAQAALDLALYILFFL
                     PGILALIYAGYGYAVESWVIGEHSTVTAEGPPIYHFKSVIPIAGALVALQGLAEIARC
                     FVCLRTGAWPARLEDVEEIDVIETQLSHSEFIDEDSRQATMKSAHAIDEAARHRSSSD
                     IEEKKP"
     gene            complement(19943..21121)
                     /locus_tag="VF182_09220"
     CDS             complement(19943..21121)
                     /locus_tag="VF182_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011976074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX6437299.1"
                     /translation="MADQAKLQKQDFKKLSRRNVLKAALAAGGVAALGFPMIARGQTG
                     PITMRWQSTWPQKDIFHEFALDFAKMVNDMTGGDLKIEVLPAGAVVPAFGLLDAVSKG
                     TLDGGHGVLVYHYGKQTALALWGSGPAFGMDANTLLAWHKYGGGKELLTKLYNSIGAN
                     VVSFPYGPMPTQPLGWFKKPITKADDFKGLKFRTVGISIDVFTGLGAAVNALPGGEIV
                     PAMDRGLIDAAEFNNASSDRVLGFPDVSKVCMLQSYHQNAEQFEILFNKTKYDALPAK
                     MKALIEHAVEASSQDMTWKAIDRYSKDYAEMQAKDKVRFFRTPDSVLQKQLVVYDEAV
                     AKKAAENPLFKEIEASQRQFAARAVKWDLDTNVGRRMAYSHYFAKGAAPAAKTEPKKS
                     "
     gene            21395..22831
                     /locus_tag="VF182_09225"
     CDS             21395..22831
                     /locus_tag="VF182_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6437300.1"
                     /translation="MDKNNLVRQLRAMVGERYVLDEKEDVIVYEQDGSIFQVMPEIVV
                     LPGDVEQVAAVVKAAKQSGVPIIPRGSGTGLAGGAVPAEGGIVLSLARLDRILQIDLE
                     NGLAIVEPGVINLDLTKAVANDGFFYAPDPSSQAACSIGGNVANNSGGPHTLAYGVTT
                     NHVLGLEIVLDDGKIVWLGGEVPDGPGYDLRGVFVGSEGTMGVVTKIIVKLMHNRESV
                     RTLLAIYDRMDDATRAVVEITGAGIIPAALEMMDRTTIEAIESGAPVGFPRDAEAVLL
                     VEIEGLREQTERSVRLAHAICEHSGAREVRLARDETERQRLWKGRKGAFGAMGSLAPN
                     YYVNDGVVPRSQLPEMMRRVAAISREFNLRIANVFHAGDGNLHPNILFDMRAPGELDR
                     VIEAGAAILNACVELGGSITGEHGVGIEKKAYIGLLFSDADLEAMTRVRAALDPDGRF
                     NPAKLFPSPAGCGEVRRKPAQIPAGLWI"
     gene            22853..24169
                     /locus_tag="VF182_09230"
     CDS             22853..24169
                     /locus_tag="VF182_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX6437301.1"
                     /translation="MEDILRNLHASDRPKIATLDIPELYQCVHCGLCLNQCPTYRVLR
                     LEPESPRGRIHLVKAAADGRIELNDRFKEHLYLCLLCRACETACPSGVQYGHIAETAR
                     AVIGPPGSKLARAILNLAFTQLLPYPGRLKILFGVLRLYQRTGLQRLVQPLLPRKIRQ
                     MDAMLPAIPQRRLPTTAKALSAIGPRRARVALLNGCVMPFLFDEINDATVRVLQRNGC
                     EVVFPKGQTCCGALNTHNGETSSAKEMARRNIDAFLTAGVDAIVVNAAGCGAAMKEYG
                     RLLEDDPVYADKARRFSSLVKDAGEFLAGIGLVGQLGELRITVTYQDPCHLAHGQRVR
                     SQPRDLLKAIPGLKLVEMNGSESCCGSAGIYNITHAEMSQQLLLEKMPAIAATGSEAV
                     VAPNPGCMLQLRYGAQKYGPNVKVYHLMDLLDRAYASGESSPADES"
     gene            24159..25466
                     /locus_tag="VF182_09235"
     CDS             24159..25466
                     /locus_tag="VF182_09235"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6437302.1"
                     /translation="MKANPDQLASRLEAELGSAAVSMEPELLFARLIDGVHPCLVCSP
                     AHAEEVSAALRVCSETDAAVTPWGGGTAVGIGNVPERVDVIINLSKLNHVIEHDDANL
                     TATVQSGISLAASQEVTAGARQFVPFDVASPRCSTIGGIVAANLNGARRACYGSVRDL
                     VIGMKVALPTGEHIKAGGKVVKNVAGYDMCKLFVGSFGTLGIITELTLRLTPVPEAEA
                     TLAASGSLMELSEFAGEILDSVLLPSAIVLWTTETSSSSETPQLGWQMAVRAEGFAEH
                     VGRHLRDLELLAERLGLATVTYTNAAHEKLWAGICDFPILGHELVYRLMVPRSAISAL
                     LHELQNLGSTDSSPAIVADMAGGTIWIGSNEVSSASARFLQLQSKAAQHKGHVALVDA
                     PAGVKRDLDIWGPTPAAFFLMRRIKQQFDPQRLLNPGRFVGRL"
BASE COUNT         6135 a         7027 c         6997 g         5422 t
ORIGIN      
        1 aaatctcgcc acaccagacg gtaaaaaaat atctttgcgt gactttcgcg gcaaaattgt
       61 gatgctgaat ttctgggcaa cctggtgcgt cccctgtcgc gaagagatgc cggcaatgga
      121 gaagctctac caggagttca aagacaaaaa cttcgtcatt ctcgcagtca atgtcaaaga
      181 tcgtaagccg gatgcggtca acttcgccaa ggagctaaag ctctcgtatc caatcgtcat
      241 cgatgcggat gctcaggttg ggctactcta cggcgcctgg ggattgccca cgacttattt
      301 gatcggaccc aagggcgaag gactggcgcg cgcttgggga ccggcggagt ggtacagccc
      361 cgcagcccgc aatctcatca aagatctcct tgatggaaaa agatgagcga tgttaacgtt
      421 tttgttgctt tcgccgccgg aattttttcc ttcctctcgc cttgcgttct cccactaatc
      481 ccatcgtaca tttcctttat atccggcgtc tcgctggaag acatgcgcca cgacaaaaca
      541 ttgaaccgag tgcgcgcgcg tgtcgtgctc aattcgatcg ccttcattgc tggtttctct
      601 ttagtgttta tttcgctcgg cgtatccgca agtttccttg gcagcctgtt cttcggctat
      661 cggaacacca ttcgaattgt cggcgggatc ttcattctgc tggttgggct gtacttgata
      721 ggtattttca agatccccgc tctggaacgg tatctgcaat tcaatttgaa ggataagccg
      781 gccggttact taggttccgt cctggtcggc atcacctttg cggtggcttg gactccctgc
      841 gttgggccga tcctcggcgc cattcttgcc ctagccggaa cttcgggaga aatcggccgc
      901 ggcactctcc tgctgtcttc ttatgctgcg ggccttggtc tgccattttt tctaagcgct
      961 gtggcgatca attctttcat gcaattttca caatcattcc gtcgttacat tcgagcattt
     1021 cacatcgcag ggggcatttt gctggtgatt gtcggcctgc ttttgataac cgattacatg
     1081 acgatgctta acatttacgc actgcgtttt acccccgact ggttgctcaa gaagctttag
     1141 ccgcccgctc cgtggagctt agaaatttga cctgtttgtg gctaaatgtt agcgttttgc
     1201 gatcgccatt gcgtgcaatc atcgaccttg ccgaccgcgt taacccggaa acataatgga
     1261 gaccgacaac agcatggaag agcagattcg attgatcttg aagtccctgg gcgaagatcc
     1321 ggaacgcgaa ggtctggtca aaacaccgca gcgcgtcgct caagcgctaa ctttcctgac
     1381 ccgaggttac gacatggatc cggcaaaggt catcaacgat gcccttttca ccgaagatta
     1441 ccaggagatg atcgttcaaa aagatatcga tttcttctcc ctttgcgaac atcacatcct
     1501 cccctttttc ggtaaagcgc acgtcgcata catcccgcac cacaaaattg tcggcatatc
     1561 caaactcgcg cgtttagtcg atgtctatgc ccggcgcttg caagttcagg aacgactgac
     1621 caatcagata gcgacgatca tcatggaaaa actcgatcca ctgggagttg cagtagtcat
     1681 cgaagccgaa catttatgca tgaggatgcg tggcgtggaa aagcaaaatt cgatggtgat
     1741 caccagcaca ttgctcggcg cttttcagaa ccggcaagaa acccgtaatg agtttatgac
     1801 gttgatccgg caacgaggtt agccgaagca ttatggaaat tagattcaaa gatatcggac
     1861 cggaaaattt gaagaccgac ctgttagtcg tgcctgtttc cgagaggagg ctcgaagata
     1921 caagcattcg cgtgctggac cggcgtttaa aaggaagact acaggagcgc atccagaaaa
     1981 gtaaatttgc cggagccgaa ggcagcatcc tgctctactc gacagccgga gcgctccctg
     2041 cgccacatct cttgttgatt ggactgggca aagccgctga tattgatgct gaagcctggc
     2101 gtagagccgg agcccgcgcc cgacgagagg ccagtgcact ggggattgag gagatcggtt
     2161 ttttcttctc tcccgaacgg gatcccgcgg acgccgccgc ggcgattgtt gaggggacat
     2221 tgttggcttc ttatcaattc acaaaatacc gctccaacgc cagaaatccg ctggcgatca
     2281 agactcttac gctccataaa tccgggctca gccgcagcag cgctatggat aaggctgtgc
     2341 gcacggctga acaaactgtc ggcggggttt tcttggcgcg ggatttgatc aacgagcctc
     2401 cctcggtcac gaccgcaacc tatttgggcg agcaggcgga gcatcattgc cgcggacgag
     2461 gtctgtccgt ggatgtctgg ggtaaaagga aaattgagtc gatgaagttg gcgggactct
     2521 tggcggttaa tcgtggcagt caagaagagc cacgcttcat caccattcat tacacgccgg
     2581 cgggtaagcc gagaaagaaa gtcgcgttaa tcggcaaggg aatcactttc gactcaggcg
     2641 gtctatcgct caagccatcg aaatctatgg aaactatgaa actggatatg gccgggggcg
     2701 cagcggttat tgctgccatg agtcggctgc cggcattgcg tccagaggtt gaggtcatcg
     2761 gctacattcc gacgaccgat aatctgcccg ggcccaacgc tcagaagccc ggcgatgtca
     2821 ttcgttacct gaatggcaaa acggtcgagg ttttgaatac cgacgccgaa ggccggttaa
     2881 ttcttgccga tgctttggcg ctagctgtca aacaaaagcc ggattacatg atcaacttgg
     2941 ctactttgac cggcgcttgc atggtcgcct tgggcacgca ggtcggtggt atattcagta
     3001 atcaccccgc tctcgcggac agaatcctgc gctgtgccca gcaagccggc gaaaagctct
     3061 ggcaaatgcc actcgtcaag gaatatcgcg aggacattaa gagcagcgtt gccgatatca
     3121 aaaatattgg cggcagtcac ggcggcgcga tcattgcggc cctgatacta caagaattcg
     3181 tggaagagat tccttgggcc catctcgaca tcgccggtcc ggcatatacc gaccgcgata
     3241 ttggcacctg tcccaaaggc ggcaccggat ttggcgtgcg tacgctgctt aaatttctta
     3301 tgaccatcta gagaggagac aaccattatg gggaaaatta cagaagtggg agacaacaat
     3361 ttcgaggcgg aagtaatcca gagtaatgtg ccggtgctgg tcgacttctg ggcgccgtgg
     3421 tgcggcccat gccggtcgat cgcacccatt gttgaggaaa tcgccggaga gtacgaaggg
     3481 aaactgaaag ttaccaaact caacgtcgac gatcacccgg ctaccgcatc gcgctacggg
     3541 attcgtggta tcccaaatct gctcatcctc aagggcggcg cggtgaaaga acagatcgtc
     3601 ggggccgtac cgaaagcaaa acttgtcagc gcgatcgaca aagcgctaca gcagcaatga
     3661 ttacagatgt ggcggggata gcccacgacc tgatgatcgt ggatgaaatt atcgcccgcg
     3721 tgatgcccgg cccgattagc gaatgtcaca gctcagctgt ggcaagctca acgcccatca
     3781 tcttgatgat cgctggctag attgcgaacc gcaatccgcc aggccgatcc ctttaaaaag
     3841 tgtagcggcg catactaacg tttatcatca tgccgatcgc gaacatcatc gacgccagtg
     3901 ccgaaccgcc atagctgaat aacggcaacg gcacgccgac taccggcatc aggcccgaca
     3961 ccatcgaaac gttaatgacg acgtgccaga acaaaattgc caccatacca aaggttaaca
     4021 acgcgccgaa tcgatcttta gctcgttcaa agagcttaag acagtaaagc atcatcgcaa
     4081 aataacaggc gagcagaaca aagcacccgg caaagcccca ctcttcggag aacaccgcaa
     4141 agataaagtc ggtatgctgt gccggcaaaa aatccagccg attttgggtg ccgtgcaagt
     4201 agcccttgcc gaacatcctt cctgagccga tcgcgatttg tgattgaatg acgtggtatc
     4261 ccgcacccag cggatcacga tcaggatcga agaacgtcag cacacgcgcg cgctgatagg
     4321 gccacaggag attccatcca attggcagaa accctaggcc gacgccggcc aaaccgagaa
     4381 acgattttat tcggaggccg ccgatcagca tcatactcat aaataccagc ccgagaatta
     4441 tggcggtgcc aagatctggc tggatcagcg tgagtcctat cagaggcgca accaatgcga
     4501 acggcgccca aagatcccgt agacagtaac cgcgggacgg ctcgctatct tggaaatatt
     4561 tcgccatcac caagacgatc gctagctttg ctggctccga cggctggagt ctgaaaaacc
     4621 cgaggttgat ccaccgctgc gagccgccgc ctgcatgtcc gacaaagaaa accagtatca
     4681 ataaaactaa catcaatccg taaaacggat acgccagccg atcaacatag tgatagtcaa
     4741 acgcaatagt cgtgaacatc accgcgagcc ccagcccgag ccaaaggagc tggcgtgaag
     4801 ggagacctcc cgcatgattc gccgcgatgt cgtaattggc gctgtagatg gtcacaatcc
     4861 ccatgccgac aaaggcaagg gtgagaagaa acagaggcca gtcgaaatgt gagatgaggc
     4921 ggcggtcaat ctgcaccgat ctctcctggc gcccgcgcct gctgttgatc ctttggctga
     4981 tttttcattt cgatatattt ttcaaaaact ttttttgcca tgggcgcagc ggcttcaccg
     5041 ccatggccgc cgtgctcaat tagcacggcg atggcaattt gggggttctc gatcggcgca
     5101 taggcgacaa accaggcatg gtcgcggttg aagtaggaga gttgctcgct tttgagatag
     5161 gctgctttca tctcgacaac ctgcgccgtg ccggttttac cagccaccga taccacgctc
     5221 gatcgcgctg ccccgccggt gccgcctttg cctctgacca catcagccaa cgccgcctga
     5281 atccggtgca aggtggctgg cttcaaatcg atggtacgaa tcttttcggg accatactca
     5341 cgtgccatcg tgccatcgac agattcgact ttattgacga tgcgcgggcg ataaagagtc
     5401 ccgccgttgg ccaccgccgc catcatgttg gccagttgca acggagtggt cgttaaataa
     5461 ccctggccga ttgcaaccga aggtgtttcg cccggatacc acggttgacg gtaacgcttc
     5521 ctcttccatg cagtgtccgg caccagcccg tccttttcat cgtcgagaaa agcaccggtt
     5581 ttttctccca acccgaatgc gcgggcatag tgggcgatct tgtctatgcc caggcgctgg
     5641 cccagctggt agaaataaac atcacaggat tccacgattg ccttgtggag gtcgaccgac
     5701 ccgtgccccc ccttcttcca gtcacggaag ctgcggttac cgaaggggaa aaaaccagga
     5761 tcagaaatcc gggcatcggg gtcgatgcct tcctccagtc cagcgatcgc catgatgatt
     5821 ttgaacgtcg acccgggtgg atactggcct tgaatcgcgc ggttattaag cggtcgcaga
     5881 cgatctttga tgagggcacg ccattcttca gatttgattc cgcgcgcaaa aatatttgga
     5941 tcgaaggccg gcgtgctgac catcgtcaag attgcgccgg tgtgaacgtc cagggcgacg
     6001 atggttcctt ccttgccctt gagcgcttcg taagctgtct gttgcagctg ccggtccaac
     6061 gttaggtaaa cggtataacc tggaacatcg accacttcgt gcagcacccg gacgcggcgt
     6121 cccagggcat caacttcaac ttgctgtcca ccgttttgtc ctttaagaaa tccttcccat
     6181 cgcttttcca agccgtactg gccgatttca tcaccggatc catacccctg ctctttaagc
     6241 agcttgagct gcttgggacc gatctcgccg agataaccca gaacatgtgc ggcgatcggg
     6301 ccgtcagcgt agttgcgccg gggccgtacc cgcaagttaa cccccggaag atcgagttga
     6361 tgcgtttcta cggctaccac cgtggcccaa tccacatccc tgcccaaaac gatttcgtcg
     6421 aatgcggcgc gactcttgtt ctcctcgaaa actgcaatga gctcattctc gggtcgtccc
     6481 aaataactcg ccagttggcg caatgtgttt tgcgggtctt gtgcatcttc ggggacaaac
     6541 aaaagatcga atgatggtcg gctgtcgacc atcaactcac cttgacggtc catgatcatg
     6601 ccacgagtgc cgggaactct cttgattcgt acccggttgt tttctgacag gtaggtatag
     6661 cgttcgccgt gggcgatctg caaaaccgcc aagcgcgaca taatgccgag aaaagcgatg
     6721 atcaggataa gataaaccat ccgcgtgcgg acgcgcagtt ccggggctcc atcttttcgc
     6781 gcaacatcgc cgagaatagc catcaataat ataagcccgg tacttggacc ttttcgagat
     6841 aggtttggcc gcggcgcaac agcgaaaaga cgaacggcgc gagcagagcg gcgaccaaag
     6901 cttcgagtag tatatacctt aaagcagcga tccagaaacc ttcactgctg agaaaaattg
     6961 ccgaaaccag cgccaacgcc acgcctttca ctaatgccgc caaaagaacg acgatggcgc
     7021 tgaccaggtg attatggagc cagattgaac gagaactgag atagacagac agaaagacca
     7081 aggacatggc tagggcgttg atgcctaaaa cggggcttga gacaacgtcc acggagtagc
     7141 ccaagacaaa cgaagccaga acggcgccga cgctcggatg atgcaaaccc cagtagacgc
     7201 aaagaaccag tatcaaatcc ggcactaaag gaaagaaatg caggaaagtc gtctgcaaca
     7261 gaaccagcag gagtcctacc gcgaacaaga gcaaggaaag tttcatgctt gcgacccaag
     7321 gacccgctac ttcgtggcgg atttcccttc cgcgctcact accagcacgg tttccgtccg
     7381 agcaggattc accgctggca tgacttcaat gacttgaaac aaacccaaag tctccttgcg
     7441 caccttagtg atcgtgccga ctatccagcc tttgaggaag actccatcga gacctgatgt
     7501 gataagccgg tcccccacct gaatatcctc agtacgtttg atgtatttca atactgtgcc
     7561 gttctccaaa gagcccgaca caatgccgcg cgcgcgagtg cgctgaacca tgacatcgat
     7621 cccgctattg ggatcggtca gcagcaaaac cttggcgctt cgcgccgccg catccacgac
     7681 acgtccaacg actcccaaag gcgtgacgac agacattccc ttgcgcaccc cgtcggcagt
     7741 gcctttgtcg atcgtgcagc tctggaacca gttgctcgcg ctgctggcaa tgatcgacgc
     7801 tgtgatgctg gccgctggta aacgcgagcg aaaatccaac aagtgtcgca gttgctggtt
     7861 ggttgcttcc gcttccaaaa atcgatttcg ctccatctca agctctaaaa tgcgctctct
     7921 gagccgctcg ttttcggcct tgtacccagc ggccgtgaga tgactctcct gaagcccctt
     7981 gatccagctc gtggttgaat acgcggcgat ctgtaatggc cgaatcagcc ataacagaag
     8041 cggtccgatc ggatcatttt taagctggcc gcgcgcggac gcgctcaaga tgtagaggga
     8101 taagacaatg cagaaacagc agctaagagg aatttggtac ttgcgaaaaa aagcgagcat
     8161 tgaaggctcg aacgcttgta ggagcaggag acattgcggg tctcgacgct agtggacggc
     8221 gacctccctg agcagtgaga tttcatccaa ggctttgccg gcgcccatca ccaccgcggt
     8281 taacggatcg tccgccatca tcacaggcaa cccggtctct tcacgcaaca gaatgtctaa
     8341 attcctgagc aacgccccac ccccagccag tacaatgccg cggtcgacaa tgtcggaagc
     8401 taattcgggc ggggttcgct caagcgaaag tcgaactgcc tcaacaatct ggttgatggg
     8461 ttccaacagt gagtctcgga tctcttcatc ggtgataacg actgtcttcg gcacccccgc
     8521 caccagatcc cgccctttga tctccatcgt ttgaatttca tcccccggat acgctgatcc
     8581 aatggttatc ttgatcagct cggcggtccg ttcgcccaca agcagattat attttcgttt
     8641 gatgaattga gcgatggctt cgtccatttt gtcgccgccg acccgaacgg agcgcgagaa
     8701 aacaataccg gacagagaaa tgaccgctac ctcagtagtg ccgccgccga tatcgacgat
     8761 catatttcct gtcggttcag tgatcggcag acccgatcca atggcagcgg ccatcggttc
     8821 ttcgatcaag tagacttcgc gtgcgcctgc agattccgcc gactcccgca cggcgcgctt
     8881 ttcaacttca gtaatcccga aagggactcc gacaatgatt cgtggtcgca ccaaagttcg
     8941 ccgattgtga actttctgga tgaagtagcg cagcattgcc tcagtaatct cgaaatcagc
     9001 gatgacgccg tcttttaacg gccggatcgc gacgatgctg ccgggcgtac ggccgagcat
     9061 gcgtttggct tccgcgccga ccgccaaaac ctttttgcct ccacgctcat ttcgttgctg
     9121 gacggcgact accgatggtt cgttgcaaac gatgccttga ttcttgacgt agatcagtgt
     9181 gttggcggtt ccaagatcga ttgcaagatc gttggaaaat accccgaaca gggcatcgag
     9241 catcataact ccttatttgc gcgaaaagag aagactaaag cgtcgtaaaa atagcagatt
     9301 tgtcactacc aagcaagaaa atgaaattgt tcagcgcgct agaaacgggt gaattttgca
     9361 atggttgcat tgccaattac ctttccctat cattattatt ctctcaagta cgctcttccg
     9421 acctaacgct gaaaggcttg tcacggatgc tcgatttcat aagaaaaaag aaacgatcgt
     9481 ggatcatcac cattctatta ggactgatca ttgtcgtctt tgttgcattc tacggcggct
     9541 ctcaacagcc caccggcgtc gccgctcacg tcgctgaagt taatggtgaa agtatctcgc
     9601 agcgggaatt tatattgcac taccagcgcg ccgtggagcg ctaccgtgag atgttcaaag
     9661 gctctctcac tccagaactg ctgaaaaact taaacatcaa gaattcgctg ctcgaagaga
     9721 tgatcgagac ccgtttagtg cttcaggaag cgcggcgttt aggtctcacc gccaccgacg
     9781 aagaactcat gaatgccatt acgcaagtcc ccgagttcca ggttaacggc cggttcaata
     9841 aggagcgcta tatacagctt ctgcgcgcca accgcttaac tcccgcgcag ttcgaagacg
     9901 accagcgaaa gcagctcacg atccagcgct tactcggaat tctcgccgat tcggcgcatg
     9961 tcactgaggc cggggtgcgc gagcgctatc gcttcgaaca ggaaaaaatc aatctacagt
    10021 tcattcggtt ttcggcgagc gagaacttat ccgaagtaaa cattagtgat gacgatatcc
    10081 agaagttcta tgaccgcaat aaggaatccc tcaaggaacc gctcaaggtt caagtagaat
    10141 acattcctta ccctttcgat cagttctcca ccccggtgca gctcaccgat aaggaggttg
    10201 aggattatta taatgccaat cggccgacca ggtttactac gcctaaacag gcaaaagtcc
    10261 gttacatcat ggtgactgtt gatcctgcag cagacgctaa acagaaggag gctgctcggg
    10321 ttcgggcgga tcggatcgtg gctgaggcgc gtgctgggaa aaacttcgcc gagttagcca
    10381 aaaaagaatc cggcgacccc agcaaagaaa agggcggaga gatcggctgg ctcaatcagg
    10441 gacaattacc ggaggctctg gacaaacaga tattcgcact ggctaaggga gaaataagcg
    10501 aacccatcga aaccccggtc ggttttcata tcgtcaaagt cgaagatatc aaggaagcaa
    10561 ggacgcaaag cttggccgag gcgacaccaa ctattacccg cgagctcaaa cttgaaaagg
    10621 gaaagtatga ggcggcaaaa atcgccgatc gcgaccgcga aaaagctgct tccggcaacg
    10681 atcttgccaa agtcgctcaa gagagcagcc tgttagtaaa gacaacccgg ctgttcagcg
    10741 agggagaagt cttgcccgag attggcccga cacaaaattt ctataagacc gccctgtcgc
    10801 tgggagcgac ggaggtcagt tcggtcatcg aaggaccgaa cgcctactac attatgaaaa
    10861 caaaagaacg aaaagaagca tttgttccgg cgctcgaggc agtccgcggt aaaatcgaaa
    10921 aaggtctgaa agaatcaaaa gcttatgaaa tggccgtgca gaaagccaac gatgtgctcg
    10981 atcaactcaa aaaggagccg aacctggcaa aactcgctcg tgataaaaaa ctgaagcttg
    11041 aggaaaccgg atggttcgct cgcaacacac aacagctgcc caaagtgggc gagctacaaa
    11101 atctgacagt cgggagtctc gcactatcgg cgagcaaacc gattcccgat aaaattttca
    11161 cgcaagctga cgcagctttt gtgtttgcgt ttaaagaggc tcaggccgcg gatatggcgc
    11221 aatatgaaaa agacaaggct caactgatgc aacaggcact cgctgagact cggcagcgga
    11281 tactcttaaa gttcaaagat gagcttaaag ccaaggccaa gatcgaagtt aattccgggc
    11341 ctcttgaaga aatctgattg cgcctttgtg ctgatgcaaa tcgttttgct tcgccatggg
    11401 gccacggatt ggaaccttca aggccgctgc cagggttcga gtgatcgcca gctcaccgaa
    11461 gtcggcctac gccaggccga gcagatcgcc actcacctca gcaacgaaac catccatgct
    11521 atttattcga gcagtctcga gagggcgcgc caaaccgcgc agctggtaag ccgcccgcat
    11581 gatcttccag tgatgatcga ggatgatgtg cgtgagctcg atcacggcga actggaggga
    11641 cttactttca acgagatcaa ggagaagttt tccgactttt taaccggttg gaggaccgcg
    11701 cccgcggaac tccaggtacc cggtggagag aggcttgccg atgtggccga gagggcctgg
    11761 agcgctgtga cacgcatcgc gcagcgtcat gaaccagatc atacggtgat cgtggtgagt
    11821 cataattttc ctattctcgg gatcgtctgc cgcctgacta gaaccgatct gaacaattat
    11881 cggttattcc atctcgatcc ctgcgggatc acccggatca ctcagaatgg aaatagtcgc
    11941 tgggaagtta ctcacgtcaa tagcaagcca tacagcttgc cgcggccgta gccgctccaa
    12001 tagcgaaccc cgattaacaa tcgacgttcg tgtttttttt ctgcttatcg ggagcgaacg
    12061 caaacgccaa gaacagcaag gcaagcatta aatagctggc tgcaaattca ggagcttcaa
    12121 acaaaccgaa acgcgcggct acgccggcgg gagtcaaatc ataggcgtgg ataagcccga
    12181 ttgcggaaaa aaccgctgcc gctaaggacc aaccggccgc ttttttaaat tgcttttcga
    12241 tcaaagcaac accgatggct gcaagaatca tcgaggtgaa aataaatcct tgctgaagcg
    12301 agatcatgcc atgcatggcg gggttttggc caaagggcgc aagtccaact gagtgcaacg
    12361 acttgccggc cacgcgcaat ccagtctcga ccattaacaa tccccaggcc gccagcgccg
    12421 gcacaattcc cagcgcgact gccggtgcat gttccttggg cgtctcctga aaagattggg
    12481 ccacgatgat aacgccgata tagagaagaa tgccaatgcc ggcttccagc ggaataaatc
    12541 caagcacgcc ttgaatcgtt ccggtaaggc aaagcaaagc gatgaaaagg ccgtttaaaa
    12601 ccgagtagcc ggagcgtgct ccgagccgct tccagcctgg gtggccgata tagatggtgg
    12661 ttgggaaaac gctgccaagc gaagccgcga ccacggatcc gaaaccattg gccagaagcg
    12721 acggcaaggc tcggtagcgg tctccagctg cttccgcgct ttcgatgttc tgcagtgatc
    12781 cgacgacgtt gaaaattccc atcgggatga tcaccgaaag ataattcaaa aatgccggat
    12841 ggcgcagcgc ctcgagtaaa gaaccaccgg tgaagtcggg gcgtgccaga gcaaagtcga
    12901 cggcgggcgg cggacctttc aaagctccta accaccatcc caacagcgtt cccacagcga
    12961 cagctaccat gccgccgggc aaaccgagcg gaaatttttg ctgtgagaaa taagcgatga
    13021 aaatcagcgc cagcggaatc agcgccacca aaggctgtgc aaagatccgg aaggtaaaat
    13081 ccatcgaaat gaaagtgatc gcgatgccgg ccaaagctga aagcaatgcc gctctagggg
    13141 ttacgccacg gacacgatcc gccacaaagg ctccggcgat ttcgatgacg ccgttaagaa
    13201 aacaggccac aaggccgact ttccatgccc aaacgggatc ctgcgtctcc tgatagatgg
    13261 gcatcatgat gaagaagata aatgcgaaca aactgacagt attgataccg taaggcagcg
    13321 cggtcacgtc atcccggccg gtatcgcgcg caagacgacg tgcctgccac gcataaaaca
    13381 ggttgccgac cagtatggaa aaggcggcgc cgggtagaat cttgccaaat atgtaatcgt
    13441 cgggaagatg gatcagttcg cggcataaac tgacgatcag gatcaactga atcagattat
    13501 cgatcatcaa gccgaaaaag ccgtcgagat ctttccggac aaaccatggt ggattgaaag
    13561 gccgttgggg catatgattt gaattcgggc ctgatggatt gggcagaatc tagcggtgac
    13621 tcctagtccc tgtcaaacgg tcctcccgcc gcccaacttg aaagataagg cggcggtgga
    13681 taagactaaa ccactgatgg cgcaattgct ttaccaggat ctgggacggt tggacttcgc
    13741 ggcagcgctc tcacgccagg aacaactcct cgagctaaag cagcgtgaag cgattcccga
    13801 tattctcctc tgcgtcgagc atccgcatgt ttataccatc ggtcgcggcg gcgacctcaa
    13861 tcacgttata gccgctcgag acgtgccgct gtatcgcacc agtcgcggcg gcgatgtgac
    13921 ttatcatgga cccggtcaac tggtggtgta tccgattatc gatctgcgtt cgaagttgcg
    13981 cagagacgtg catcgttatg ttagaaatct cgagatgacc gcgatcgaaa cgctcaagga
    14041 tttcggcatc gaagccgcgc gtcatccgcc gtttaccggt atctggtgcg ataaccgaaa
    14101 gatagccgcc atcggcatcg ccgtgcgccg atgcatcacc ttgcacggct tagggttgaa
    14161 cgtgaacacg gatcttagct atttcaatcg catcgttcca tgcgggctgg cttgggccga
    14221 ggtgacttcc atggccaagg aactggacaa agagctaagc gcgaggaacg taagagaaag
    14281 ttttcttcgc cacttcgccg atctgtttgg ctatacggaa atcgaaatta tttctcattt
    14341 ttcagaattt caggctggtc aaaaaggcgc cagctacgag gcgcgcgatg atcgatgagc
    14401 ggaggtgtac gtgttagtac gttggagcga atcgggcgag cgcaacgaag cagatggtcc
    14461 tgcttcatca gccggctaag gaactatgcc gcgacgacat ccagagtgga tcaaagtcaa
    14521 atcccccggc agtcccaatt atctgcgtct caaacgtatt ctgcgcgaaa aaaatctgca
    14581 tacggtttgt gaggaagcgc gctgccccaa catcggcgaa tgctggggca acaggactgc
    14641 gacttttctc attctcggcg atacctgcac gcgtggttgc cggttctgcg ccatcgataa
    14701 gggcaaaccc ctggcgctcg atcccgagga accgcgcaat gtcgcgcggg tcgttaaaga
    14761 tctcggcttg gatcacatcg tggtgacttc cgttaaccgc gatgatctcc cggacggcgg
    14821 ttcgagccat tttgcaaaga cggttttttg gattaagagc ctcagcccgg gcattcgtgt
    14881 cgaactgcta atccccgatt ttgacggtaa tctgtcggct gtgaaaacgg tcgtcgactc
    14941 cgggattgag atcctgaatc acaacatcga aaccgtgccg cgtctttacg gcacggtccg
    15001 acccgggcac agttatgaat gctcgctgaa cattttgaaa agtgccaaag aaatgcgccc
    15061 ggacgtgttg accaagtcgg gccttatgct cggggtcggc gagggctatg aagaggtgat
    15121 cgggaccttg cgcgacttgc gtcggaacga agtcgatatc gtaacgctag ggcaatatct
    15181 gcagccctcg aaccgccagc tcaaagtaga ccgctacgtc acgcctgatg agtttagtga
    15241 atttaaggtg gccgccgaaa aactcgggtt tcgtcatgtc gagtcaggtc cgctggtgcg
    15301 cagctcgtac catgcgtgga gtcacgtaaa ctaggaaagc tgaaaacgaa accctgaaca
    15361 gcgtcctggt ttgggggtgt gaagtttagt cttttagtct cgggtttagc attgccggtt
    15421 tacggctgac ctctccgact gtgacgttct tgccgcggct gaaaccgatt cgcagtatca
    15481 cctttcagct gtccgatttc agctttcatc ttagcttttc tccgccgcta tgtgctgggc
    15541 cagccaccgc gccgttcgca acagccgcgc atcaccataa cgggggccaa ccagttgcac
    15601 gccgagaggc atgccggtgc tgctctgcaa caatggcaaa ctcagtgcgg gcatgccgca
    15661 aagtgtccag agcgtgcaga acaatggatc gccggtgttg gcaagacctt tcggcgctgc
    15721 gctgggcgcg gcgggagtga gaatcgcgtc atagcgttgt tcgaaaagct caagaaaact
    15781 atcatggacc aagttgattc gcccaagcgc cgagaggtaa tccgccgcgc gcacttcgcg
    15841 tccccgctcg atctgtgccc gcagttgctc cgatagacga gagccgctgt tcttccatgg
    15901 gcgctcgaga ttgtacgcca tttcagcgcc catgatactc tggtgccact gccaagcgtt
    15961 caccgccgat ggaaacaatt ctacttcttc gacctgccca ccgagatgct cgattatttc
    16021 ggcgaaacct tcgcgggtat cttcgtcggc gctttgccaa ttaggagttt tgataaatgc
    16081 gaacatcggc gccaacggcg gttcttccgc ggcaatctcg gccaacggga gacgcgcccg
    16141 cggccgggtg tcagcgtcgt tttggtcgta accggcaata tgttcggcga atagagcgat
    16201 gtcggcaact gaattagcga acacgccgac atgatcgaga gtgcgcgaca gcgtcaacgc
    16261 gccatggcgc gaaatcagtc cgtgagtcgg cttgaatccg atcacgccgc aatacgccgc
    16321 gggacgaatg atcgaaccgt tggtttggct tccgagcgcc agcggcacca tgtttgctgc
    16381 cactgccgct gctgaaccgc tggaagagcc gcccggcgtg tgctcagggt tgtgcgggtt
    16441 gcgtgttttg ccaggtgaga aataggcaaa ttctgtggtt acggtcttcc ccatgattac
    16501 cgcgccggcg gcgcgcaaca gggccgtcac ggtggcgtcg cgcaacggag tgcggccgcg
    16561 gtaaagcact gagccgcact ccgtaggcat gtcggcggta tcgaaaatat ctttgatgcc
    16621 aacgggaacg ccgtgcagcg gaccgatcgc tccgccagat aaacgccgtt cgtcgagcgc
    16681 acgcgcctgg gccagagcat agtcctcatc cagaaacgtc caagcttgta cctgcgggtc
    16741 gacttcccga atacgcgcaa ggcaaccctc gacgagctgt tccgagttaa tgataccatc
    16801 gcggatcaat cgggctgcct cggtcgcaga aagcaagtgt agattggtta gttccatcca
    16861 gcgtatttac aatcagaggt gctggttgaa ttgaattaga aaatctaaaa acaaagcttt
    16921 ccgtttttct cggttccagg cttcatcgcg aaagcggctt tcgatattgt cattagattc
    16981 cgcgaccgtt cgtctgcgct gtggctacaa gcgagcagtc caaggcccga gggagaaggg
    17041 caccggaaac ggtcatcctc agtaggttga ggatgatcga ggaccgagaa cgcaggaatg
    17101 cgaagcttat ccccacagca ctaaccgtac agaaattcgg gtagccacag cgtcatgccc
    17161 ggccaaatat acatgcagat caagcacagg accacgatca gcatgtaagg catcatgccg
    17221 gcaaaaattt gattgagggt tacatgcggt ggcgataccc cctttaaata aaatgccgac
    17281 atcgccaccg gcggcgatag aaaggccgcc tgcaggttga ctgccaccat ggtgccgaac
    17341 aagatcggat ccacctgaaa atggccgagc agcgggataa agatcggaca gaaaatgacg
    17401 atgatctccg tccattcgag cggccagccg agcaggaaaa taatcatttg tgagatgatc
    17461 aggaagccga ctggcgaaag attcatgctg agcacccagc gttcgatgag cccttggccg
    17521 ccgtgcagcg caaagacgct ggagaacagc gccgagccga taaagagcca gcaaaccatg
    17581 gcggtagttt tagcggttaa gaaaacggac tgcttgatat tggttcgtag ttcgagcgat
    17641 ttcgggaggt agatgaaaag cggcaccgcg gtgccgataa aggtggcgcc gagcgatgac
    17701 gccacgacca aagtcgcgac gtcaccacgc ccgagtccga ggatgaatcc cgctatcgct
    17761 cccaacatgc tcgaccaagc gcagacgcgc ggatacttca ccatcacggc gagatagaga
    17821 gccccaaggg cgccgatggc ggccgactca gtcgcggtgc agatgccgaa caggattacc
    17881 actaggacaa ccacagtgag aacgcccaat ggcaccactg agataacgag ttgttttagg
    17941 atttcaaatc cctcaccgtc catgcggtag taataaatcg acagcagcag tgtcgatact
    18001 gcggcgaggc tccagaacca taggtaaaaa tgctcgggaa ctcgtcccga cgcgggtacg
    18061 ccgggggatt cagccaagga tcccatctcc tcgggcggcg cttcggccag cggagcgggt
    18121 tcggacggca tcgtggtcgt ctcagcgctg ccaggctcct cacctgcgct cccgagctct
    18181 tgtaacgttt cagcggcttg cccaagttct tggggtttgt tctctacggg cgaagctgtg
    18241 gactcatggg gcaccgactg catttcaacg ctcggcgcca ccgatgcggc gggcgctgca
    18301 ttgtagattg tcacatacca ccaggtggta agaaaaactc caatggtcaa cgtcaacggc
    18361 actaacagta cgagaatatt cttaacgagg aacgagtaac ccaccggttt gccagcgatc
    18421 gtagtcgcgc cgtgcagaag atgcggttga aggactgccg ccaggagttt agtagcgatg
    18481 cgttttgtgc cgccaccgga tagcaactgc agccattgag gcggtgtaat ccggtactgg
    18541 tccgaggaaa gctggggtgc gatctttgga ttgatcatcg cccaccccag tatgtaaacc
    18601 aaatacaaaa atgtaaggaa aaacccgggc agcatcgccg ctgcataaag tttcacgatc
    18661 gactgtccgg caaccgcggc atagacgatc agcatcactg agggaggaat aagtatgccg
    18721 agagtcccac cggctgtgat cgcgccggag gccagccgca catcatagcc ggcgttcaac
    18781 atgggacgca tggcgatgac gcccatcagg acaacgacgg cgccaacaat gccggaagca
    18841 attccccaga acgcacacac gagcagggtc gtaaccgcca gcgatgccgg aacgcgtcgg
    18901 aacgcaagct gcacgctgtg gaacatgcga tcgaccagcg cggcgcgttc catgatatag
    18961 cccatgaaca cgaataacgg caccgagagc agagtgtcgt tgaccatgac gccataggtg
    19021 cgttgcacca ttagatcgaa gatcctgtta tcccaccagt gtgccgccgg atcaaagaag
    19081 gccaaaaagc caaacagcat acccaacccc atcagtgtga atgcggtcgg aaaacccatc
    19141 atgatcgcaa tcacgatcag cccaagcatc gtcaagccga gcgcaggatc gctcatggct
    19201 ttttttcctc gatatcgctt gagctgcgat ggcgggctgc ttcatcgatg gcatgcgccg
    19261 acttcatggt cgcttggcgc gagtcttcgt cgatgaattc gctatgcgat agctgcgttt
    19321 cgatgacgtc gatctcctcg acatcttcca ggcgcgccgg ccaggcgccg gtgcgaaggc
    19381 agacaaaaca gcgggcaatt tccgccagtc cctggagcgc gacgagagcg ccagcaatcg
    19441 ggatcactga tttaaaatga tagatcggcg gaccttccgc cgtgactgta gaatgctccc
    19501 cgataaccca agattccacg gcgtaaccat agccggcgta aatcagcgcc agaatcccgg
    19561 gcaggaaaaa caggatataa agcgccagat ccaaagctgc ctgcgcgcgt gggcgcaaat
    19621 agatatagac aaaatcggca cgcacatgtc ccgccagcgc cagcgtgtaa gcgccgcaca
    19681 tcatgaacaa tgtgccatat agcatgctat tgaagtcgaa gatccaggcg gtgggggcgt
    19741 ttagaatata gcgcttgaat acctcgaccg acacgacaag cgtcagcgcc atgatcagcc
    19801 aggcgaacgc tttgccggtc caatagctga cctgatcaat gcctcgaatt aacgcttgaa
    19861 cgttcataga agacaaaaaa gccagccatc cgttgagcgc gaatggctgg ccagctcggc
    19921 tttttgaaaa atcacggagt ggttagcttt tcttcggttc cgtcttcgct gccggagccg
    19981 cgcctttggc gaagtaatga ctgtaggcca ttcttcgtcc aacattagta tccaagtccc
    20041 actttaccgc gcgtgccgcg aactggcgtt gcgatgcctc gatctccttg aacaacgggt
    20101 tctcggccgc ctttttggcg acagcttcgt cgtagaccac gagctgtttt tgcagcaccg
    20161 aatcgggagt tctgaagaac cggactttat ccttggcctg catctcggca tagtctttcg
    20221 aatagcggtc aatcgccttc cacgtcatgt cctgtgatga cgcctctacc gcatgctcga
    20281 taagcgcctt cattttcgcc ggcagggcat cgtacttggt cttgttgaag agaatttcaa
    20341 actgttcggc attctgatgg tagctctgta gcatgcagac tttggacacg tccgggaacc
    20401 ccagaacgcg atctgaggag gcgttgttga actcggcggc atcgatcagt ccgcgatcca
    20461 tggcaggcac gatttctccg ccgggcagcg cgttcaccgc cgccccgagc cctgtgaaaa
    20521 cgtcgatcga aataccgacc gtacgaaact tcaatccttt gaaatcatcc gccttggtga
    20581 tcggcttttt gaaccagccg agcggctggg tgggcatcgg tccataggga aatgagacca
    20641 cgttggctcc gatggaatta tagagcttgg tgagcagctc cttgccgccg ccatatttgt
    20701 gccaggcgag gagcgtgttg gcatccatgc cgaatgcggg tcccgacccc cagagcgcca
    20761 acgccgtctg cttgccgtaa tgataaacca gcactccatg gccaccgtcg agtgtaccct
    20821 tggaaacagc atcaagcagg ccgaaggccg gtacgacggc ccccgccggg agcacctcaa
    20881 tcttcaagtc gccgccggtc atgtcgttga ccatcttggc gaagtcaagc gcaaactcgt
    20941 gaaagatgtc tttctgcggc catgtgcttt gccaacgcat cgtaatcggt cccgtttggc
    21001 cgcgggcaat catggggaat ccgagcgccg ccacgccacc tgcggcaagt gccgctttga
    21061 gaacgtttcg ccgtgagagt tttttgaaat cttgcttctg gagtttcgct tgatcagcca
    21121 tggattcccc tttcagataa gcactaccac gaaatagcgt gcacggttta tattacaggt
    21181 tgcggattta cgtcaatggt acctccagcc tgcacaaact cacaagcgat tcagactgat
    21241 aatttgtatg tctcggctca ccgaggttgt gccgagttag catattgaca agtgagcatg
    21301 attgccgcag gcaacttctt ggtctgtctc attgacactc tattcgactc tcggctaggc
    21361 tccgaacgga tccccggaag aaacaagaaa cgaaatggat aagaacaatc tagtgcgcca
    21421 gctacgcgcc atggttggcg agcggtatgt cttggacgaa aaggaagatg ttatcgtcta
    21481 tgaacaagac ggctcaatct ttcaggtcat gccggagatc gttgttctgc ccggagacgt
    21541 cgaacaagtt gccgccgttg taaaagcggc caaacagagc ggtgtgccga ttataccgcg
    21601 cggctcgggt acgggtttgg ctggcggtgc cgtgccggcc gagggcggaa tcgtgctgtc
    21661 gttggcgcgc ctggatcgta tcctgcaaat cgatctagag aacggccttg cgatagtcga
    21721 gcccggcgtt atcaatctcg acctcaccaa agccgttgcc aatgacggct tcttctatgc
    21781 tcccgatcct agcagccagg ccgcttgctc gatcggcggt aatgtagcca acaattccgg
    21841 aggtccgcat accctcgcct acggggtgac aaccaatcac gttcttggtc tcgagatcgt
    21901 tctggacgat ggcaaaatcg tttggctggg tggcgaagtg ccggacggcc ccggctatga
    21961 tcttcgcggt gtgtttgtcg gctccgaagg aaccatgggt gtggtgacta agataatcgt
    22021 aaaactcatg cacaaccgcg aatcggtgcg cacccttctt gcgatctatg accggatgga
    22081 tgacgccact cgggcagtgg ttgaaatcac cggcgctgga atcattcccg cggcattaga
    22141 gatgatggac cgcacgacca ttgaagccat cgaatccggc gcgccggtag gctttcctcg
    22201 cgatgccgaa gcggtgctgc tggtcgaaat cgagggcttg cgcgagcaaa ccgagcgttc
    22261 cgtgcgattg gcgcacgcca tctgtgagca cagcggcgcc cgtgaagtcc gcttggcgcg
    22321 ggacgaaacc gaacggcaac ggctttggaa aggcagaaaa ggcgctttcg gcgccatggg
    22381 ttcgttggcg cccaattact acgttaacga cggcgttgtc ccgcgtagtc aactgccgga
    22441 gatgatgcgg cgagtggctg cgatcagcag agaattcaat ctacgaattg ctaatgtatt
    22501 tcacgccggc gacggcaatc tccatccgaa tattctcttc gacatgcgcg cgccgggaga
    22561 actcgatcga gtcattgaag cgggcgcggc aatattgaac gcatgcgtcg agctcggcgg
    22621 cagtatcact ggcgagcatg gtgtaggcat tgaaaagaaa gcctacatcg gtttgctatt
    22681 cagtgatgcg gatctggagg cgatgacgcg agtccgcgcg gcgctggacc cggacggccg
    22741 gtttaatccc gcaaaactat ttccatcgcc ggctggttgc ggcgaagtgc ggcgaaagcc
    22801 ggcgcaaatt ccggcggggc tatggattta gaaaaacctt ttagagttta tcttggaaga
    22861 cattctgcgc aacctacacg ctagcgatcg accgaagatt gccacgctcg atattcccga
    22921 actctatcaa tgtgtgcact gcggtctatg cctgaaccaa tgcccgactt accgagtgct
    22981 tcgccttgaa ccggaatcgc cacgcggcag gattcacctg gtgaaagcag ccgcggatgg
    23041 ccgtatcgag ctcaacgacc gtttcaaaga gcatctttat ctatgcttgc tgtgccgcgc
    23101 ttgtgagaca gcctgtccat ccggcgtcca gtacggccat atcgccgaaa ccgcccgggc
    23161 ggttatcggt ccgccgggat cgaagctcgc aagggcgata ctgaatctgg cttttaccca
    23221 gctgcttcct tatcctggac gtctaaaaat cctgttcggc gtgttgcgtc tctatcaacg
    23281 aaccggcttg cagaggctgg ttcagccgtt attgccgcgc aagattcggc agatggatgc
    23341 aatgctgccg gccatcccgc aacgccgtct cccaacaacc gctaaggctc tttctgcaat
    23401 cggcccacgg cgagcccggg tcgcgctctt aaacggctgc gtgatgccgt ttctctttga
    23461 cgagatcaat gacgccaccg tgcgcgtgct gcaacgcaac ggctgcgagg ttgttttccc
    23521 gaaggggcag acctgctgtg gcgcgctgaa cacccacaat ggcgagacga gcagcgcgaa
    23581 agaaatggcc cggcgcaaca tcgacgcgtt tttaaccgcc ggcgtcgacg ccatcgttgt
    23641 caatgccgcc ggctgcggcg ccgcgatgaa agaatacggc cgccttctcg aagacgatcc
    23701 tgtttatgcc gacaaggcgc ggcgcttcag ttccttagtc aaggatgccg gagaattcct
    23761 tgcgggtatc ggattagttg gccaactcgg cgaattgcgg atcaccgtta cctatcagga
    23821 tccctgtcat ctggcgcatg ggcagcgcgt gcgcagccag ccacgggatt tattgaaggc
    23881 gattccgggt ttgaaattgg tcgagatgaa tggctctgaa agctgttgcg gcagtgccgg
    23941 aatttacaat atcactcatg ctgaaatgtc acagcagctt ctcctagaga aaatgccggc
    24001 catcgccgcc accggttcgg aagcggtggt cgcgcccaat ccaggctgta tgctgcagct
    24061 ccgatatggc gcgcagaagt atgggccgaa tgtgaaagtc tatcacctca tggatctgct
    24121 cgatcgcgcc tatgcgagcg gcgaatcttc gccagccgat gaaagctaac ccggaccagc
    24181 tcgccagccg gttagaagcg gaacttggca gcgccgcggt atcgatggag cctgaattgc
    24241 ttttcgcccg gctcatcgac ggagttcacc cctgcctggt ttgttcgccg gcccatgccg
    24301 aggaagtgag cgcggcgtta cgagtttgca gtgaaacgga tgcagccgtc actccctggg
    24361 gcggcggcac ggccgttgga attgggaatg tacctgagcg tgtcgatgtc ataatcaacc
    24421 tgtccaagtt gaatcacgtc atcgaacatg acgacgcaaa tttgactgcc acggtgcaga
    24481 gcggcatttc cttggccgca tcacaagagg ttacggccgg agcccggcag ttcgtgccct
    24541 tcgatgtggc cagcccacga tgctccacga tcggcggcat tgtcgcagcc aatctcaacg
    24601 gagcgcggcg cgcctgctac ggtagcgtgc gtgatttggt gattggtatg aaagtagccc
    24661 tgccgacggg ggagcatatc aaggccggtg gcaaggtcgt caaaaacgtc gccggctatg
    24721 acatgtgcaa acttttcgtt ggctcgttcg gaacgctcgg gatcatcacc gaactgacac
    24781 tcagactgac accggtcccg gaagcggaag ctaccttggc cgcttccgga agtttaatgg
    24841 aactttccga gtttgccggc gagatcctgg attcagtttt attgccgagc gcgattgtgc
    24901 tctggacaac tgaaacaagt tcgagcagcg agacgccgca gctgggctgg caaatggctg
    24961 ttcgagccga aggttttgcc gaacatgtgg gccgtcatct acgggatctc gaattgctgg
    25021 ctgaaagatt gggcctggca accgtgactt atacgaatgc ggctcatgaa aaattatggg
    25081 cgggtatttg cgattttccg attctcggcc acgagctggt ctatcgtttg atggtaccac
    25141 gatcagcgat tagcgctttg ctgcacgaac tccagaactt gggttcgacg gattctagtc
    25201 cggcgattgt cgcggacatg gcgggcggca cgatctggat tgggtcaaac gaagtcagtt
    25261 cagcgtccgc gcgattttta cagctccaat ccaaggcggc tcaacacaaa ggtcacgttg
    25321 cgctggttga cgcgccggcc ggggtcaagc gggatctgga tatctgggga ccgactccag
    25381 cggcattttt cctaatgcgc aggattaaac aacaattcga tccccagcga ttgctgaatc
    25441 cgggccgctt tgtcggtcgc ctatgaaccg ggagtatcac cgaaagatcc acaccaccca
    25501 attgccgatt aagttatcca gaccgtaggc gccgcttccc cgcacggcaa agtaaataac
    25561 gccggctaac aaaactaaat t
//
LOCUS       DASUVS010000165         6881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1157749, whole genome shotgun sequence.
ACCESSION   DASUVS010000165 DASUVS010000000
VERSION     DASUVS010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157749"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..228)
                     /locus_tag="VF182_09240"
                     /pseudo
     CDS             complement(81..228)
                     /locus_tag="VF182_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076920.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal
                     protein"
     gene            complement(265..1524)
                     /locus_tag="VF182_09245"
     CDS             complement(265..1524)
                     /locus_tag="VF182_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX6437303.1"
                     /translation="MSILVALALVCLILLLSIAIWSIRRHRDPKLHIECDSPIDELIP
                     SLAGLTLGTAVSGNSVDVLENGAFFDVLLEQIRSAQKSVHLETFLWKEGALGKRMADA
                     LSERARAGKQIRVLLDAEGSKDAGKAALRQMKEAGCQVVLFHEKTLRNIGVLNDRDHR
                     KIVVIDGRQAFVGGHCIVDTWLGDAQDNQHFADITVRLRGPIVHTVQSAFSENWAGET
                     GELFVGDDVFPMLQPEGDVLIHAAYAKPESSAPAVKILHHTAICLARKRIWIQNPYFI
                     PEPEAIDAFGVAVSRGVDVRVLMPSTAGSDNPMVQHAGHRNFEKLLRCGVRLFEYPHT
                     LLHQKVMTIDGVWSAVGSSNFDDRSFETNDEITLGILDAATARRLDAFFEKYVSRCEE
                     IKLEAWRKRGLWHKAKDNAFYMLNEVL"
     gene            2164..2385
                     /locus_tag="VF182_09250"
     CDS             2164..2385
                     /locus_tag="VF182_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437304.1"
                     /translation="MKFLTPPFGTKNNGLNPDHQRLQAGRRAQGDRLGDWRTFPREKL
                     EGTGSHELAIWPAPRGAMSALEKKPKAID"
     gene            complement(2429..3436)
                     /locus_tag="VF182_09255"
     CDS             complement(2429..3436)
                     /locus_tag="VF182_09255"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437305.1"
                     /translation="MKRKSAFLLITSAIFLAFLFLPRVSAGQSLPKIRMAYTSIAIQF
                     TPVYLMKELDLGRKQGLDVEILMIPVSSRAVQSALAGELHFITSGGVANINANMAGAD
                     FVGLTSTINTLVYKILGQPSIKEPGQLKGKKIAISRLGGVSDFSVRFGLDRWGLVPDK
                     DAALIQIGGDPEAYLALQNKAVDAAALSEPFATMAQREGYSLVSDLSRLNIPFTHHGI
                     GARKQLIRDHRETAVRFLRAYLEGIYVFKTNKELSLKTFQKVARLSDLALVQGIYDEY
                     SQRLIPAVPYPTSAGIQTIIDQLAKNRPQAKTLNPNDFIDVSILKEIEDSGFIKRLYG
                     K"
     gene            complement(3791..4822)
                     /locus_tag="VF182_09260"
     CDS             complement(3791..4822)
                     /locus_tag="VF182_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6437306.1"
                     /translation="MRSLRLLCSGLIAGLILFQWLSIARAAPLDELVGAARKEGTLNF
                     YGPSTLKPAGAQVLAQAFNKRYGTNIEVKFIPSGSMTRDVGKVATQAATGAPPEWDVM
                     VATDAHHASLWLRKLQKPFDYKSLGVDEKLIDHDGGAISFVHQYIVPAYNSSLVAPSD
                     APKSWEDLLQPKWKGKVGVTTATHHLGRLALGPWGEEKTTAYVTALAQQNPVVGELGP
                     TYTRLQLGEILVALSLTDSYIHVAKQSGAPISEAVEVQPVVAPSYHIGVLKGAQHPNA
                     AHLFAVFMTMPESQQVWEKTVAVSSALIPGTAYYQKAQGKQMIYMRKDQSEKVESLAR
                     KYGKMIGLL"
     gene            complement(5227..6279)
                     /locus_tag="VF182_09265"
     CDS             complement(5227..6279)
                     /locus_tag="VF182_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6437307.1"
                     /translation="MNQKKSSLSSIGSATFISTIALAVLICGASAGKLFAAAADSLDQ
                     LYEKAKKEGKVTLYAPLSPSAMSVIPPAFMKRFPGITVDHIDATSDKLIARVLAEQKG
                     GRVIADVISGALTYLPQILDLKIVERVVIPEAAAYPAQLKGDLWVATDTQFYIAGWNT
                     NLVKKGEEPKSYEELANPKWKDSIMGEPRDYQMLVGFAKRKYNNDEKAIELFKKIAAN
                     QVEFHRGHSQLAEFLMAGQRAICFTCYSHHFPMRVKKGAPIQPLMTEGVGEVGGSAMV
                     MKEAPHPNAALLWVRWAVSEEGQKVYAQAGETPAHPKVEPLEKIRPTTPYMLTIDDVR
                     EFPKYEKTWKEIFQIR"
     gene            complement(6510..>6881)
                     /locus_tag="VF182_09270"
     CDS             complement(6510..>6881)
                     /locus_tag="VF182_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437308.1"
                     /translation="SYPQHAVTTLRPTIAKTPQMIEGVLKALIESVAIFKTNKQKSFA
                     VWRKYMRGASEEFLEETYQHTIAGLESVPTPSVQVIASALDMISAHYPQAKQTDPNLI
                     VDPSFVRRIDQSGFIAALYKK"
BASE COUNT         1522 a         1905 c         1859 g         1595 t
ORIGIN      
        1 acaccagtgc agtttgactc accaggcgtt gtggtctcat cggggccacc ccgcaagcgg
       61 taagacatct cctatcgcag aaggacaaat cgggtcggcg aacacactga aggattggcg
      121 tacgcatctg tgagcaggcc tattgcggca agccgagcaa gaacagacaa gcactcggct
      181 ctgcggccgt agcagccgag atcccgcata ccctagatca tctgagacgt cgctcccggc
      241 tcattcaagc gagccggtaa tctgttagag aacttcgttg agcatataga aagcgttgtc
      301 tttcgctttg tgccataatc cgcgcttgcg ccacgcctcg agcttaatct cctcgcaacg
      361 ggatacgtac ttctcgaaga acgcatcgag ccgccgtgcc gttgccgcat cgagaatgcc
      421 cagggtaatt tcatcgttgg tctcgaaaga ccggtcgtcg aaattacttg aaccgacggc
      481 gctccagaca ccgtcgatgg tcatgacttt ctggtgcagc agagtgtgcg gatactcgaa
      541 caaacgtacg ccgcagcgca gcagcttttc gaagttccgg tgcccggcgt gctgcaccat
      601 cgggttatcg gagcccgctg tcgaaggcat gagcacgcgc acatcgacgc cgcgggaaac
      661 cgccaccccg aacgcatcga ttgcttcggg ctcgggaatg aaatagggat tctgaatcca
      721 gattcgtttg cgggcaaggc agatggcggt atggtggaga atcttcaccg ccggcgccga
      781 gctttccggc ttggcgtagg ccgcatggat caacacgtcg ccctcaggtt gcaacatcgg
      841 aaatacatcg tcaccgacga acaactctcc cgtttccccc gcccaattct cgctgaacgc
      901 ggactgcacg gtgtgcacga tggggccgcg caatctgacg gtgatgtcgg cgaagtgttg
      961 gttgtcctgc gcgtcgccca gccaagtgtc gacgatgcag tgaccgccga cgaatgcttg
     1021 gcgcccgtcg atgaccacga tctttcgatg gtcgcggtcg ttcagcacgc cgatgttgcg
     1081 caaagtcttt tcatggaaaa gcacgacttg gcagccggct tctttcatct gtcgcagggc
     1141 tgccttgcct gcgtctttgg agccttcggc gtcgagcagg acacggatct gcttgccggc
     1201 ccgagcccgc tccgagagtg cgtcagccat gcgcttacca agcgcgcctt ccttccacag
     1261 aaaggtttcc aaatgcaccg acttctgggc ggatcggatc tgctccaaca gcacgtcgaa
     1321 aaacgcgccg ttctccagga cgtcgacgga attgccagag acggctgtcc ctagagtcag
     1381 cccggcaagc gaaggaatca gctcatcaat cggcgaatca cattcaatat gcagcttggg
     1441 atcgcggtgg cgtcgaatcg accagatggc aatcgacagc agcaaaatca ggcagaccaa
     1501 tgccagcgcc acaagaatgc tcatggctat gtactttatg gggcgggagt caggggagca
     1561 agtcgtcgct ggtgcgctgg cagagcgctc catgcacgtt ggcgagtaag tgatttcagc
     1621 gattgaaaat cttcgctctt cctcagggga gcctcacact tgattcgaca agctcaccag
     1681 gagcgggtcg gttttcccgt cattctaagt cacgcaaaaa ggctataaaa gattaattgg
     1741 ctggaatgtt ggaacagtta tttaaggcgg accttctgcc gctcgatggg tggtgccgaa
     1801 tcattcatcc aagataaacc cgcgatccgc gcgattagat cgagacccaa aatgtcaaat
     1861 aaagaacacc ggtcagagta aatcctggct tctcgcagtt acgtagagct gcttccgggc
     1921 aatcaattca gctcctaagc cgaaaagtat caccaaagta gccacgtcga caatctgcgc
     1981 gggaggcacg gttgaatggc tgtccggccc tctccgaagg ggacgcgctc tgcgacgccg
     2041 ggatcgcgac gatgcgaatc aacgcggcga tacccgcggc ggtgataatt cgctccttta
     2101 agccgtctcc tccaattttt aggctagcaa atagcaatcc gggcagaaaa acgtcaccgt
     2161 taaatgaaat ttctcactcc gccgtttggc actaaaaata atgggcttaa tccggaccac
     2221 cagcgactgc aagcgggtcg ccgagctcaa ggagaccgcc tcggggattg gcgaaccttc
     2281 ccgcgcgaga agttggaagg cacgggcagc cacgagctgg cgatctggcc ggcacctcgc
     2341 ggcgccatgt ccgccctgga gaaaaagccg aaagcgatcg actaggtcga gcctttcacg
     2401 ctcatacagg atcgagaact ctcctgatct atttcccgta caagcgcttt ataaagccgc
     2461 tgtcctcaat ttctttcaag atagacacgt cgatgaagtc gttggggttg agggtcttgg
     2521 cttgcgggcg gttttttgcg agttggtcga taatggtttg gatgccagcg gaagtggggt
     2581 atggcacggc cgggattaaa cgctggctgt attcatcgta aatgccttgg accagggcaa
     2641 gatcgctcaa tcgcgcgact ttctggaagg ttttgaggga caactcttta ttggtcttga
     2701 aaacgtagat gccttccaga tacgcgcgca agaagcgcac cgcagtctca cgatggtcac
     2761 ggatcagctg tttgcgcgcg ccgatgccgt gatgtgtaaa cgggatattc aagcggctca
     2821 ggtctgaaac aagagaataa ccttcacgct gcgccatcgt ggcaaaaggc tcggatagag
     2881 ccgcggcgtc gacagctttg ttttgcagcg ctaggtaggc ttccggatcg ccgccgatct
     2941 gaataagagc ggcgtctttg tcaggcacca gtccccagcg atcaagaccg aagcgcaccg
     3001 agaaatcgct cactccgccc agccgtgaaa tggcaatttt ctttcccttg agctgtcccg
     3061 gttctttaat cgagggttgg cctagaattt tataaacaag cgtattgatc gttgatgtta
     3121 agccgacaaa atccgcgccg gccatattgg cgttgatgtt ggccacaccg ccgctggtta
     3181 tgaaatgaag ttcgccagcc agagccgact gcaccgcccg cgagctcacc gggatcatca
     3241 agatttcgac gtcgagaccc tgctttcgtc cgagatcgag ctccttcatc aaataaaccg
     3301 gcgtgaactg aatcgcgatc gacgtataag ccatgcggat cttcggcaga gattgcccgg
     3361 cgctaacgcg cggtaagaac aagaacgcca aaaaaatcgc tgaggtgatt aggagaaaag
     3421 ccgactttcg cttcattgta ttgctcttcg gtcctatgtt cgctcaactc tatcttttga
     3481 ttacctttcc gagatcgctg ctgctcatct ccactccagc cctacgttga gggtcggagc
     3541 aaaactaact tatcgacagc tcttaccaag acaacaaggt ctcccgaata tcgcaaattt
     3601 tttatgacgt cattgacatc cgtgtaaaca atcaatcgat accatctccg atcatgttcg
     3661 acttgttaga atagtagcgg cggtggcgat cgtcgaaaga tcctcctgga gatcgcggcg
     3721 ctctcgagct ggcccaacgc gatagccagc ggcttagtcg ctaacttaat cacgtgagga
     3781 gtgatctccg ctacaaaaga ccgatcatct tgccgtattt tcgcgcaagc gattctactt
     3841 tctcagattg atccttgcgc atatagatca tctgcttacc ctgggccttt tgataataag
     3901 ccgttccggg aatcaacgcg gagcttacgg cgacggtttt ttcccacacc tgttgcgatt
     3961 ccggcatcgt catgaagact gcgaagaggt gcgccgcgtt gggatgctgc gcgcctttga
     4021 gcacgccaat gtgataggac ggagcgacta ccggttgcac ctcgacggct tcgctgatcg
     4081 gagccccgct ttgtttggcc acgtggatgt agctgtcggt caaactcaac gcgacgagga
     4141 tctcgcctag ttgaagccgg gtatacgtag gccccagctc tccgacgacc gggttctgct
     4201 gcgccagagc tgttacatag gcggtggtct tttcttcgcc ccagggtccc aaagccagcc
     4261 gtcctaaatg gtgtgtcgcc gtggtaacac cgactttccc cttccacttt ggttggagga
     4321 ggtcctccca gctcttcggc gcatccgaag gcgcaaccaa actgctgttg taggccggca
     4381 cgatgtactg atggacgaag gaaatcgcgc cgccatcgtg atcgatcaat ttctcgtcca
     4441 cgcccaaaga tttgtagtcg aacggttttt gcagcttgcg cagccagagg ctcgcatggt
     4501 gggcgtctgt ggcaaccatg acatcccatt caggcggagc gccggtggcc gcctgagtcg
     4561 caaccttgcc cacgtcccga gtcatcgatc ccgacggaat gaatttgacc tcgatgttgg
     4621 tcccgtatct tttgttgaag gcttgagcga gaacctgagc gcccgcaggc ttcaaggtgg
     4681 acgggccata gaagttcagc gtgccttctt ttctggcggc gccgaccaat tcatcgagcg
     4741 gcgcggcgcg agcaattgag agccactgga acaaaatcaa gccggcaatc agtcccgagc
     4801 aaaggagacg cagcgatcgc atctttcacc tccggttacg tgccgtggcc ctttccagga
     4861 gccgtctggt aaatgcgccc gagctctttc ctggacggcg tctatcgctg gtcctgagaa
     4921 ttgtcaagtc gatatttcac gctgtcctct tgctcgcgcc tatttgacgg aatgcaagcg
     4981 atggctatgc cgagcggcag cccggaaagc gaaggaatca gttcttagat cggcgagcta
     5041 aattcactgt gcagcttggg atcgcggtcc cgtcgattag cgagatggcg atcgatagcc
     5101 gaaaagcaag ttatccaatg ccacccctaa aatgttcatc atatgtacct ttgagatcta
     5161 gtgcaccgga gcaagtcatc gacagcttgc cgcgcgccgt cggcaataaa gaccgctcgt
     5221 cgaatttcaa cggatctgga agatttcctt ccacgttttc tcgtacttcg gaaactctct
     5281 tacgtcgtcg atcgtcagca tataaggcgt cgtcgggcga attttttcca gaggttccac
     5341 tttgggatga gccggagttt cacccgcttg agcataaacc ttctgtcctt cttcactgac
     5401 cgcccagcga acccagagta gtgcggcgtt gggatgcgga gcttccttca tcaccatcgc
     5461 agagccgccg acttcgccca caccttccgt cattagcggt tgaatcgggg cgcctttttt
     5521 cactcgcata ggaaaatggt gtgagtagca agtaaaacaa atggcgcgct gaccggccat
     5581 gaggaactcg gctaactgcg aatgcccccg atgaaactct acttgattgg cggcgatttt
     5641 cttgaacagc tcgatcgcct tttcatcgtt gttgtatttt cgtttggcaa agccgaccag
     5701 catttggtaa tcgcgcggct cgcccatgat gctatctttc catttgggat tcgcgagctc
     5761 ctcgtagctt ttcggctctt cgcctttctt caccagattg gtgttccatc cggcaatata
     5821 gaattgcgta tccgttgcca cccaaaggtc gcctttcaac tgggccggat acgcggccgc
     5881 ctcgggtatg acgacgcgct ccacgatctt taggtccagg atctgcggca aataggtgag
     5941 cgctccgctt atgacgtcgg caatgacgcg tccgcctttc tgctccgcga gcactcgcgc
     6001 gatcagcttg tcgctcgtgg catcgatatg atcgaccgtt ataccgggaa agcgcttcat
     6061 gaacgccggc ggaatcaccg acatcgcgct aggggatagc ggtgcataaa gcgtaacctt
     6121 tccttccttt tttgctttct cataaagttg atcgagggag tccgctgcag ccgcgaaaag
     6181 cttaccggca gaagcgccgc agataaggac agctaaggcg atggtcgaga taaaggttgc
     6241 agatccgatc gaactcaatg aactcttctt ttgattcatt tcactcctcc gtgatcttca
     6301 ttccatgatg ctgaatcaat atccgggaaa attttccctg ccttgcgact cttcgtaaac
     6361 ccttctctgc aactcctcca tgtaggaaaa taccgaagat gtcaaactta ggccaggcat
     6421 tcatgtttcg ctcatcccac taggtagttc caaattaaac cactaagttt tgcgagaggg
     6481 attcgcggct gcgcgcagat ttgggtgagt tattttttat ataaggcggc gatgaatccg
     6541 ctctggtcga tccgtcggac gaacgatgga tcgacgatga gattcggatc ggtttgtttt
     6601 gcttgagggt aatgggcgga aatcatgtcg agcgcactgg caatcacctg cacggaaggt
     6661 gttggcaccg actccagtcc cgcgatagtg tgctggtagg tttcctcgag aaactcctcg
     6721 ctcgcgcccc gcatgtattt acgccaaaca gcgaaactct tttgtttatt ggttttaaaa
     6781 atcgccacgg actcgatgag agccttcaaa actccctcga tcatctgtgg tgtcttcgca
     6841 atcgtcggtc tgagggtggt tacggcatgc tgggggtagc t
//
LOCUS       DASUVS010000166         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1376709, whole genome shotgun sequence.
ACCESSION   DASUVS010000166 DASUVS010000000
VERSION     DASUVS010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376709"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VF182_09275"
     CDS             <1..330
                     /locus_tag="VF182_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437309.1"
                     /translation="SRLPDTPLLSELMDEVKTPDLGRRVATVMLGSGELGRPVVTSPA
                     TPNDRIQLLRDAFMKSMSDPTLLEEAKQKKLDITPVPGEELAKIAREVIDQPQDVVDR
                     IRKILAQ"
     gene            429..1556
                     /locus_tag="VF182_09280"
     CDS             429..1556
                     /locus_tag="VF182_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX6437310.1"
                     /translation="MKIADTEILELQNIPVTPPLFKQPVRTAVRLLKVKTDDGRVGIS
                     QLGGFMHSATSAFIQQDLLPFLKGKDPLENERLMHQMLWKFNTRAHAGVWNFAASAVD
                     VALWDLKGKFYNAPVWRLLGGAQKAIPSYITFGLRAYNRDELAEAAKHWVANGQSRLK
                     IQVGRSNIRGEMDPSGASGEHREDNPAEDESRIRAVREAVGDDVELMADANCLMKYDA
                     ALRWCKRFEPYNLMWFEEPIIHNDPHLLAELRKQTSIPIAAGQWDNFFKLADLVKQNS
                     VDFLNIHVLSVGGFTMGMKAAGLAHAFNLPVGNGDHFDIHLHAAVPNGWRAEIHVNNW
                     LTANVVYKDLPGPVKGWITLTEKPGLGLELNDDAIKEFQVR"
     gene            1597..>2161
                     /locus_tag="VF182_09285"
     CDS             1597..>2161
                     /locus_tag="VF182_09285"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6437311.1"
                     /translation="MKQRMGIGIVVFLSGLYFGSVSPSLAQESFYKGKSIRLIVGSTP
                     GGFYDRWARFFARYMPKYIPGNPEISVQNMPAAGSLVAANYVYNVAKPDGLTVGVVQY
                     NIYMDQLVGRKEVQFDVRKFSWIGSPASESVLLYMRKDAPYQSIRDIIKAKEPPKCGS
                     SGTVSTDYLLARLLEDSIGAKFNTVIGY"
BASE COUNT          506 a          570 c          617 g          468 t
ORIGIN      
        1 tcgcggctgc cggatacacc gttattgagc gaactgatgg acgaagttaa aacgccggat
       61 ctcggacgac gagtggcgac ggtcatgctc ggttccggtg aactcggccg gccggtagtg
      121 acgtcgccgg ccacgccaaa cgatcgcatc caattgctac gcgatgcctt tatgaaaagc
      181 atgagcgatc caacgctgct ggaggaagca aagcaaaaaa agctggatat taccccggta
      241 cccggcgaag agctggcgaa gatcgcccgc gaagtcatcg atcagccgca ggatgtcgtc
      301 gatcgcatcc gaaaaatact ggcgcagtag gacacgggga gagtggtgcc tctgctaaat
      361 cgccaggatc atcttcgccc tcatcgtgcg gctgtttaat aacgcgcgaa ttccaaagga
      421 gaaatacttt gaaaatagct gataccgaaa tcctcgagct acaaaatatt ccggtgactc
      481 cgcccttgtt caagcagccg gtgcgcacgg ccgtaaggct attgaaggtc aaaaccgatg
      541 atggccgcgt cggtatttcc cagctgggcg gctttatgca ttcggcgact tcggctttta
      601 ttcaacagga tctgctgcct tttttgaaag gcaaggatcc gctggagaac gagcgcttga
      661 tgcatcagat gctctggaaa ttcaacaccc gcgcccacgc cggagtatgg aattttgccg
      721 ccagcgccgt cgatgttgcg ctctgggatc taaaaggaaa attttacaac gctcccgtgt
      781 ggcgtctatt gggcggggcg caaaaagcca tcccgtccta tatcaccttc gggctgcgcg
      841 cgtataaccg ggatgagctt gccgaagcgg cgaagcattg ggtcgccaac ggacagagcc
      901 gcctcaagat tcaggtcggc cgctccaaca tccggggtga gatggatcct tccggcgcca
      961 gcggtgagca ccgagaagac aatccggcgg aagatgagtc tcgcatcaga gctgtgcgcg
     1021 aggcggtggg cgatgatgtt gaattgatgg ccgatgccaa ctgtttgatg aaatacgatg
     1081 ccgcactgcg atggtgcaaa cggttcgagc cgtacaatct catgtggttc gaagaaccga
     1141 tcatccacaa cgatccgcat ttattggccg agctgcgtaa acagacgagc atcccgattg
     1201 ccgccggaca gtgggataat tttttcaagc tcgccgacct ggtaaagcag aattccgtag
     1261 acttcttgaa catccatgtg ctttccgtgg gcgggtttac catgggcatg aaagcggcgg
     1321 gactggcgca cgcgttcaac ctgccggtgg gaaatggcga ccatttcgat attcacttgc
     1381 acgcggccgt tccgaacggc tggcgcgccg agattcacgt caataactgg ctcacggcaa
     1441 acgtcgtcta taaggactta cccggaccgg tcaaaggctg gataactctg accgaaaaac
     1501 caggactcgg cttggagctt aacgatgacg cgatcaaaga atttcaagta cggtaagtcc
     1561 ggagttcagg tgaagtgcac atcttgcgga ggagtcatga agcagcgcat gggtattggt
     1621 atcgttgttt tcctctcggg tctttacttc ggttctgtct cgccctcatt ggcgcaagaa
     1681 tcgttctaca aaggaaagtc catccgcttg attgtcggct caactcccgg agggttctat
     1741 gaccgctggg cgcgattctt tgcccgatac atgcccaagt atattcccgg caatcctgag
     1801 atttccgtgc agaacatgcc tgcggccggg tctttggttg cggcaaacta tgtctacaat
     1861 gttgccaagc ccgacggact cacagtggga gtggtgcagt acaacattta catggaccag
     1921 ctggtggggc gtaaggaagt gcagttcgac gtccgcaagt tcagctggat cggctctccg
     1981 gccagcgaaa gcgttttgct ttacatgcgc aaggatgccc cctatcaatc gatccgagac
     2041 atcatcaagg ccaaggagcc gccgaaatgc ggctcctccg gaaccgtaag cacggactat
     2101 ctgctggcga ggttgctcga ggattccatc ggagcgaaat tcaatacggt tatcggttat
     2161 c
//
LOCUS       DASUVS010000167         8656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1595709, whole genome shotgun sequence.
ACCESSION   DASUVS010000167 DASUVS010000000
VERSION     DASUVS010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8656
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595709"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..2046
                     /locus_tag="VF182_09290"
     CDS             190..2046
                     /locus_tag="VF182_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6437312.1"
                     /translation="MYGSQPADRSVHPVSNSRSLIFRLKGTTIYRYYLRIKFLIDRYR
                     PQLILGVLSLIGTDLTGIALPWLIKEGIDAAVEKELAGVSLIDFPLLILLAAIFQGFF
                     RYCWRININGFSRKAEADLREKVFSHLQKLPLSYFQHNKTGDLMSRLTIDIQAVRELL
                     GFGSLASVDAIITIIFSLACMIYIDPWLTFWALLAMPMITITVRTFGRHIFRWSHSVQ
                     EQLSDISAYVQENLAGMRVVQVYAQEDNQIRGFQKISAECMRKNMWLAILWGIFWPLM
                     RVVSGIAATIVLWLGGRQVLQGTMTLGEFVAFNGYLGMLTWPLMAIGYVVNQYQRGTA
                     ALTRIGEVLDAEPSPRYDVSVFAKQQSAIELAIEFRNLTFTYRDNGPTLKNITLAVPA
                     GNVCALVGETGSGKSTLVNLLLRLYEPPSGTIFINGMDITKMPPRVLTDSIGYVSQDI
                     FLFSGSIRENILFGTNPVDEAVIERASSAAQLLPAVQEFPDKFETIIGERGVRLSGGQ
                     KQRTALARAIVKDPPILILDDAFSSVDAETEETILARLRDFMAGRTTLLISHRIATVR
                     DADLIVYMRRGEMIEKGRHYELLQRKGAYYELYRRQQLVREVESLDRMDGRL"
     gene            2043..3890
                     /locus_tag="VF182_09295"
     CDS             2043..3890
                     /locus_tag="VF182_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6437313.1"
                     /translation="MSQDPHAEDEILGKAYDARLMRRILRYLQPYWKLLAVALVFLIF
                     HTGTELLRPYITKIAIDRYIAAGDVDGLDTMVWLYLGTVIAGFVFLFIQTYSTEYAGQ
                     RAMHDLRMQIFTHLQRQDMAYFDRNPVGRLMTRTVNDVETLNELFSTGVVGLLGDVFI
                     VFGIAGAMLWLDWKLALIALSAFPVIIYASRFYRRRAREVYRESRLILARLNARLQET
                     IAGVGTIQAFGQEGRMYDRFQEINAGYRNVLLRSVRYNAVFFPIIEIFSALSIGLVLW
                     YGGWSIGIDGLQAGVIVAFIQYLQRMYQPIRDLAEKYNIMQAAMASSERIFTTLDSQP
                     TIQNPAAPVSPAKLRGAVEFKDVWLSYGNGEPILKGISFSAHPGERIALVGATGGGKT
                     SIVSALCRFYEIDRGAILVDGVDIRDWDKQALRRQLGLVLQDVFLFSGDIASNITLGD
                     ERISDARMIEASRRAQIAPFIEKLPGGYRAEVQERGSTLSQGQRQLLSFARALAFDPR
                     ILILDEATSSVDTETEMLIQTALEELLKHRTAIIVAHRLSTIKNADRILVINKGQIWE
                     QGGHDELLQRAGLYARLYDLQYRLERLNGATAEASSAAEPLSADQAERS"
     gene            complement(3864..5084)
                     /locus_tag="VF182_09300"
     CDS             complement(3864..5084)
                     /locus_tag="VF182_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX6437314.1"
                     /translation="MDPSRRSFLKSVGLGFVALGLPPTFLVRAAGAEQNGGGKVLVVV
                     FQRGGMDGLNVVVPFKDPAYYKLRPSIAVSEPATGEDRAIDLDGFYGLHPALAPLKSI
                     YEKEHLAVIHAAGSPDNTRSHFDAQDYMEIGTPGVKSTPDGWLNRSLAGRSSAPMSLR
                     GVAMTARTPRMLAGETPVLAISSIDEFRLRNEQLNSSLQKLYAQSTDPLFRRGGESLF
                     QAMATLRQVQATIPESTATFPNGRFGQSMKQIARLIKSNVGLEIAFAEIEGWDTHVNE
                     GGAKGQLANRLKELGDGLTAFYQDLGDRMEDVVVLTMSEFGRTARENGNRGTDHGHGN
                     VMFALGGNVRGGRVLGRWPGLAPEVLYEGRDLDLTTDYRAVCSEVLQKHLGLKSSTAV
                     FPGFRPSGALGLIG"
     gene            complement(5085..6497)
                     /locus_tag="VF182_09305"
     CDS             complement(5085..6497)
                     /locus_tag="VF182_09305"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX6437315.1"
                     /translation="MMAPSLTQQQKFVHFLNRTSFGPTRDDFERLQHSGIAAYLDQQL
                     SPEQISDSIVADKVAELKTMRLSSRELLELYPQAAVAQAQGMDTAQMQGPRQVILELQ
                     LARLSRAVYSRRQLYEVMVDFWSNHFNIYAAKGADRWLSTSYDRDTIRPHALGRFKDL
                     LTATAQSPAMLFYLDNWMSVSPQSPAAQRGGNNRRRGINENYAREVMELHTLGVDSGY
                     TQKDIQEVARCFTGWTIERPRGEGEFRFEQRFHDNGPKTVLGTHIASGGGMEDGLKVI
                     DILANHPATAKFIVTKLARRFVADDPPPALVSKASHVFQQSGGNIAAVLRAIIDSPEF
                     FSSEFYQGKVKKPLEYVASALRITDAETKITHPLLRYLNRMGEPLFLAQPPTGHPDTG
                     ASWISPDMLLTRMNFVSDLLGNRLPGARVRKDAVSDPELIIGLIGPESISPGTRAAMN
                     ETKGAESLALLLAAPEFQRR"
     gene            complement(6497..7123)
                     /locus_tag="VF182_09310"
     CDS             complement(6497..7123)
                     /locus_tag="VF182_09310"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEX6437316.1"
                     /translation="MICLVILLAFLLAPGASWSQSGGRDESATQRNREAGDRWQGMTP
                     EQKQKLRDRWKSLPPEQKAEMRKKFERWRSMSAEEKASARKNFERWQQLSPEDRERLR
                     DRWQQWRELPPESREALKRRLERFRELPPDTRQELREKARERFEKMSPEEKQQRRERF
                     RERMEKLSPEEKSALRENFRERKQREAEEPGTLRKDLRDPSQKPREKD"
     gene            complement(7334..7834)
                     /locus_tag="VF182_09315"
     CDS             complement(7334..7834)
                     /locus_tag="VF182_09315"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6437317.1"
                     /translation="MSMVCAELEQDLVLYHYGDLSGASRDRLATHLRACPGCATYLKE
                     LTALLPLTVNTDEPGEDFWQNYSREMRHKLAELQEKKSWWQELQEVVWPWTLPVLAGT
                     AVVALALTLNTANKTTVIDDGSTADQALMEVLPMAENLELINNMDVLENIDVLEVMEG
                     SGNEAA"
     gene            complement(7843..8478)
                     /locus_tag="VF182_09320"
     CDS             complement(7843..8478)
                     /locus_tag="VF182_09320"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6437318.1"
                     /translation="MAFALQGCALTVDGAFEQCMETAPDKPRDEALVILAQKGDSKAF
                     EELVERYKQKAFRIAFDFSRDRDEAKDLSQEAFLRAYTHLKNFDGRSGFYTWFYRILV
                     NICLDYRRKTKRASAEEFNETIESQVEPSHPAAHSVSPDQQAIAAQMSRQVDAALDAL
                     PPRQRMAFVLKNHQGLSIREIAEMMETAEGTVKVHLHRAVTALRIRLAEFA"
BASE COUNT         1770 a         2432 c         2396 g         2058 t
ORIGIN      
        1 gtgatagtaa ttcggccgcc gatgaaaaag tcaccattcg cgatcgtaat accatgcaac
       61 aaattcgcgt gccgctggct agcgtggaag cggtttttac gaaactgatg gccggacatt
      121 ggaacgatgt tgcccgaagc ccaggcgttt caatagtgaa ccctgcagac tgatctaagc
      181 tccggcatcg tgtatggatc tcagccggcg gatcgcagcg tccatccagt ctcaaacagt
      241 cgttctctaa tctttcgatt aaaaggcacg acgatttacc gttactatct tcgcatcaag
      301 tttctcattg accgttatcg gccccagctc attctcggag tactatcgct gatcggcacc
      361 gatctgaccg gaatcgcgct gccctggctg atcaaggaag gcatcgatgc ggcggtagag
      421 aaggagctcg cgggcgtttc gctgatcgat tttccgctgc tgatcttgct ggcggccatt
      481 ttccagggtt tttttcgcta ctgctggcgc atcaacatca acggtttttc acgcaaagcg
      541 gaagccgact tgcgcgaaaa ggttttttct catctgcaga agctcccgct cagctatttc
      601 cagcacaata agaccggcga cttgatgtcc aggctgacca tagatattca ggccgtgcgc
      661 gagttgcttg gcttcggttc gctggccagc gtcgatgcca tcatcacgat cattttcagt
      721 ctcgcctgca tgatctatat cgatccgtgg ctcacatttt gggcgctgct ggcgatgccg
      781 atgatcacaa tcacggttcg caccttcggc cggcatatct tccgctggtc gcattcggtg
      841 caggaacagt taagcgacat cagcgcttat gtccaggaga atctcgcggg catgcgcgtg
      901 gtccaagtct atgcgcagga ggacaatcag attcgcggtt tccagaagat cagcgcagag
      961 tgcatgcgca aaaacatgtg gctggcgatt ctatggggaa tattctggcc gctcatgcgg
     1021 gtggtctccg gtatcgccgc gacgattgtt ctatggctcg gcgggcgcca ggtgctgcag
     1081 gggacgatga ccctcggcga gtttgtcgcc ttcaacggct acctgggcat gttgacctgg
     1141 ccgctaatgg cgatcggcta cgttgtcaac cagtatcagc gtggcaccgc ggccctgact
     1201 agaattggcg aggttttaga cgccgaacca tcgccgcgtt acgacgtatc cgtttttgct
     1261 aaacagcaaa gcgcaatcga gttggcaata gagtttcgta atcttacctt tacttatcgc
     1321 gacaatggac cgactctgaa aaatatcacc ctcgccgttc cggcgggcaa cgtatgcgcc
     1381 ctagtcggtg aaacgggttc gggaaaaagc acgctcgtca atttgcttct gcgtctctac
     1441 gagccgccgt ctggcacgat cttcattaac ggcatggaca ttaccaagat gccgccccga
     1501 gtgctgaccg actccatcgg ctacgtctcg caagatatct ttcttttttc ggggagcatt
     1561 cgcgaaaaca ttctgttcgg gacgaatcca gttgatgaag cggtgatcga aagggcgtcg
     1621 agcgcagcgc aacttctgcc tgcggttcag gaatttccgg acaagttcga aaccatcata
     1681 ggcgagcggg gcgtgcgact ctccggcggg caaaagcagc gcaccgcatt ggcgcgggcg
     1741 attgtcaaag acccgccgat cttgatccta gacgatgctt tttccagtgt cgatgccgaa
     1801 accgaggaga cgatccttgc gcggctgcga gattttatgg cgggccgaac aaccttgctg
     1861 atttctcata ggatcgccac cgtccgcgat gccgatctga tcgtttacat gcgccggggg
     1921 gaaatgatcg agaaaggccg ccattacgag ctgctccaac gtaagggcgc ttattacgag
     1981 ctataccgcc gtcaacaatt ggtgcgcgaa gtcgaaagcc tggatcgaat ggacggccgg
     2041 ctgtgagcca agatccgcac gcggaagacg aaatactggg caaagcttac gatgcccgtt
     2101 tgatgcgccg catactgcgt tatctgcaac cgtattggaa gctgctcgca gtggcgctgg
     2161 tatttttaat cttccataca ggaacggagt tgctccgccc ctatatcaca aaaatcgcga
     2221 tcgaccgtta tattgcagcc ggcgacgtcg acggtttaga tactatggtg tggctctatc
     2281 tgggcacagt gatcgccggt tttgtttttc tgtttattca aacttatagc accgaatatg
     2341 cgggccagcg ggcgatgcac gatttgcgga tgcagatatt tactcatctg cagcggcaag
     2401 acatggctta tttcgatcgt aatcccgtcg ggcgtctgat gactcgcacc gtcaatgacg
     2461 tcgagacact gaatgaattg ttcagtaccg gcgtggtcgg tttgttgggc gatgtgttca
     2521 tagtttttgg aatagccggc gcgatgttat ggctcgactg gaagttggcc ttgatcgctc
     2581 tgtccgcttt tcccgtgatc atctatgcat cgcgctttta tcgacggcgc gcgcgcgagg
     2641 tgtaccgcga gagccgtttg atccttgcgc ggctcaatgc ccggctccaa gaaaccatcg
     2701 ccggcgtcgg caccatccag gcttttggtc aggaaggcag gatgtacgac cggttccagg
     2761 aaattaacgc cggttaccgc aatgtgctcc tgcgcagcgt gcgctacaac gcagtattct
     2821 ttccaatcat cgaaatcttt agcgctctct caatcggcct ggtgctttgg tatggcggtt
     2881 ggtcgatcgg catcgatggc ctgcaggcgg gtgtcattgt ggcttttatt cagtacctgc
     2941 agcgcatgta ccagccgatc cgggatttgg cggagaaata caatatcatg caagccgcca
     3001 tggcgtcgtc ggaacgcatt tttaccactc tggatagcca gccgaccatt cagaatcctg
     3061 ccgcgccggt atcaccggcc aagctgcgcg gcgccgtgga gttcaaagac gtctggctgt
     3121 cttacggcaa tggcgaaccg attctcaagg gcatctcctt cagcgctcat cctggcgagc
     3181 gaattgcgct ggtgggcgcc accggcggcg gtaagacgtc gattgtctcg gcgctttgtc
     3241 gcttctacga gatcgatcgc ggcgcgattt tagtcgatgg cgtggatatc cgggactggg
     3301 acaaacaggc gttacgccgg caactcggac tggttctgca ggatgtcttt cttttttccg
     3361 gtgacatcgc cagcaatatc actcttggcg atgaacgtat cagtgacgcc cgcatgatcg
     3421 aagcgtcgcg gcgcgcgcag atcgcgccgt tcatcgagaa gctgccgggt ggttatcgcg
     3481 cggaagttca ggagcggggt tcgaccttgt cccaaggaca gcggcagttg ctttcgttcg
     3541 cgcgcgccct ggccttcgat ccgcgcattt tgatactgga tgaagcgacg agctcggtgg
     3601 acaccgaaac ggagatgctg atccagacgg cgcttgaaga gttgctcaaa caccggaccg
     3661 cgatcatcgt cgcgcaccgt ctttcaacga tcaaaaatgc cgaccgcatt ctagtcatta
     3721 acaaagggca gatttgggaa cagggcggac acgatgaatt gctgcaacgc gctgggctgt
     3781 acgcgcggct ttatgatttg cagtaccgct tagagcgact aaacggcgcg accgctgagg
     3841 catctagcgc cgccgaaccc ctatcagccg atcaagccga gcgctcctga agggcggaat
     3901 cccgggaaca ccgctgtcga agatttaagc ccgagatgct tctggagcac ttcgctgcaa
     3961 acggcgcggt aatcggtggt caggtcgaga tcgcgtcctt cgtaaagaac ttccggagcc
     4021 aagcccggcc atcgacctaa aactcggccg ccgcgaacat tgccaccaag cgcgaacatc
     4081 acgttgccgt gaccgtgatc ggtgccgcga ttgccgtttt cccgcgccgt gcggccaaac
     4141 tccgacatcg ttagtacgac gacgtcttcc atccgatcgc cgagatcttg ataaaaggca
     4201 gtgagaccgt cgcccagttc ctttaaccga ttggctagtt gacctttcgc gccgccttca
     4261 ttgacatggg tatcccatcc ctcgatctcg gcaaaggcga tttccaatcc gacgttgctt
     4321 ttaatcagcc gggcaatctg tttcatgctt tggccgaagc ggccgttagg aaatgttgcg
     4381 gtgctttccg gaatagtcgc ttgtacctgg cgtaacgtag ccatggcttg aaaaaggctt
     4441 tcaccgcccc gacgaaacag cggatcggtg ctctgggcgt agagtttctg caggctgctg
     4501 tttagctgct cgttgcgcag acgaaattca tcgatggacg aaatagctaa caccggcgtt
     4561 tcgccggcga gcatcctcgg cgtgcgcgcg gtcatggcga ccccgcgcaa cgacatgggc
     4621 gcgctggacc ttccagccaa agatcggttc aaccacccgt cgggtgtgct ctttaccccg
     4681 ggcgtgccga tctccatgta gtcctgcgca tcgaagtgcg agcgcgtatt gtccggcgac
     4741 ccggcagcat ggatgaccgc caaatgttct ttttcataaa tgctcttgag cggggcgagc
     4801 gcgggatgca atccatagaa gccatcaaga tcgatggctc tgtcttcgcc tgtagccggt
     4861 tccgatacag cgatgctggg acgcaattta taataggcgg gatccttaaa cggcacgacc
     4921 acatttaacc catccatgcc gccgcgctgg aacaccacca ccaggacttt ccccccgcca
     4981 ttctgctcgg cgccggcggc ccgaaccaga aatgtcggcg gcaatcctaa cgcgacaaag
     5041 ccaagaccaa ctgatttgag aaacgatcgg cgtgatgggt ccatttatct cctctggaat
     5101 tccggcgctg caagcagaag agcgagcgat tcggcgcctt tggtttcgtt catagcagcg
     5161 cgagtgccgg gtgaaatcga ctctggcccg atcaaaccaa tgatcaactc aggatcgctc
     5221 acggcgtcct ttcggacacg cgcgccggga agacggttgc caagcaaatc gctgacgaaa
     5281 ttcatccgtg tcagcaacat gtccgggctg atccaggacg cccccgtatc aggatggccg
     5341 gtgggcggct gcgccaggaa cagcggttcg cccatgcgat ttaaatagcg caacaaagga
     5401 tgcgtaattt tagtctcggc atcggtaatc cgcagcgcgc tggcgacata ttcgagcggt
     5461 ttctttacct taccctggta gaactcgctg gaaaaaaact cgggagaatc gatgatcgcg
     5521 cggagtaccg ccgcgatgtt cccgccgctc tgttgaaaca cgtgggaggc tttgctgacg
     5581 agtgctggcg gcggatcgtc agcgacaaaa cggcgcgcta actttgtcac gatgaatttt
     5641 gccgttgccg gatgatttgc cagaatatcg atcactttga gaccgtcttc catcccgccg
     5701 ccggacgcga tgtgggtgcc gagcacagtt ttgggaccat tatcatggaa gcgctgctcg
     5761 aagcgaaatt cgccttcgcc gcgcggacgc tcgatggtcc agccggtaaa acagcgggcg
     5821 acctcctgga tatccttctg cgtataacca ctgtcaaccc cgagggtgtg cagttccatc
     5881 acctcgcggg catagttttc gttgatgcct cgccgccggt tattccctcc ccgttgagcg
     5941 gcaggacttt gcggactgac gctcatccaa ttgtccaaat agaaaagcat cgccgggctt
     6001 tgcgccgtcg cagttagaag atctttaaac cgcccgagag catggggtcg aatcgtgtcg
     6061 cggtcatatg atgtcgatag ccagcgatcc gcgcctttgg ccgcataaat attaaaatga
     6121 ttgctccaga agtcgaccat gacttcataa agctggcggc gactgtaaac tgcgcgactc
     6181 aatcgggcaa gctgcagctc gagtatcacc tgccgcggtc cctgcatttg cgccgtgtcc
     6241 atgccttggg cttgggcaac ggcggcttgt ggataaagct caagaagctc gcggctactc
     6301 aaacgcatgg ttttgagctc agccactttg tcagcaacga tcgaatcgga gatttgctca
     6361 gggctgagtt gttggtccag atatgcggcg atccccgagt gctgaagtct ttcgaagtcg
     6421 tcgcgtgttg ggccgaagct tgtgcgattg agaaaatgca caaatttttg ctgttgggtg
     6481 agcgatggcg ccatcattaa tccttctcgc gcggtttctg cgacgggtct ctcaggtcct
     6541 tgcgaagagt tcctggttct tctgcttcac gctgtttacg ttcacgaaaa ttttcgcgca
     6601 gcgcgctttt ttcttcgggc gatagtttct ccattcgttc gcgaaaccgc tcgcgccgtt
     6661 gctgtttctc ttcgggggac attttctcga atcgctcgcg cgccttctcc ctgagctctt
     6721 gtctggtgtc cggcggcagt tcgcgaaagc gctcgaggcg tcgttttaga gcttcgcggc
     6781 tctcgggcgg cagttcgcgc cattgttgcc aacggtcgcg caagcgctcg cgatcctcgg
     6841 gagagagctg ctgccaacgc tcgaagttct tgcgcgccga ggccttctcc tcggctgaca
     6901 tgctgcgcca gcgctcaaac tttttccgca tttccgcctt ctgctcaggc ggcagcgatt
     6961 tccaacggtc gcgcagtttc tgcttttgct ctggcgtcat cccttgccag cggtcacctg
     7021 cttcacgatt tctttgcgtt gccgattcat cgcgtcctcc cgattggctc cagctcgcgc
     7081 caggcgccag aaggaaggcc agtagaataa caaggcaaat catcgctgac ttcattttag
     7141 cgtttctttt ctatagacgc ggcgatagct cggcgatcgt gtacatcccc atccatccgt
     7201 ccatctgggg tttgcgtttg ccgcaaccgg tttcacggcg cgttgcattg cgatcacccg
     7261 ctgcttgccc ttggttcgtc gctcgaccgg agtcgcgtag caagatagca gcggaagctg
     7321 agcgcctggt aagttaagcg gcttcgttgc ccgaaccttc catgacctcg agaacgtcga
     7381 tgttttcaag aacgtccata ttgttgatta attccaggtt ctccgccatc ggcaatactt
     7441 ccatgagagc ttgatccgcc gtggacccgt cgtcgatgac ggtcgttttg ttggcagtat
     7501 tcaacgtgag ggctagggcg acaaccgctg ttcccgccag caccggcaat gtccagggcc
     7561 agacgacctc ctgcaattct tgccaccagg attttttctc ttgtagctct gccagtttgt
     7621 gacgcatttc acggctatag ttctgccaga agtcctcgcc cggttcatcg gtgtttaccg
     7681 tcagcgggag tagagccgtc aattccttca ggtaagttgc acaccccggg cacgcgcgca
     7741 ggtgggttgc gagccggtca cgacttgcac cacttagatc gccataatgg tagagcacca
     7801 gatcttgttc aagctcagcg caaaccatac tcatgaatcc tcctaggcga actcggccaa
     7861 tcttattctg agcgccgtca ccgcgcggtg taaatgaacc ttgaccgtcc cctcggcggt
     7921 ttccatcatt tcggcaatct cacgaattga tagcccctga tgatttttta gaacgaatgc
     7981 cattcgttgc ctcggcggca aagcatccag tgccgcgtcc acctggcgcg acatctgggc
     8041 agcgatcgcc tgttgatcgg gtgaaacgga atgggctgcg ggatggctgg gttccacctg
     8101 actctcgata gtctcgttaa actcttcggc tgaagctcgt ttggttttgc gccggtaatc
     8161 cagacatatg ttcaccagaa tcctatagaa ccaagtatag aaacctgagc ggccgtcgaa
     8221 atttttcaga tgcgtatagg cgcgaagaaa cgcctcttgc gacagatctt tggcttcgtc
     8281 acgatcgcga gaaaagtcga aagcgatacg aaacgctttc tgcttgtaac gctccaccag
     8341 ctcttcgaat gctttggaat cgcccttttg ggctaagatc accagcgctt cgtcgcgcgg
     8401 cttgtccggt gccgtctcca tgcactgttc aaatgcacca tcaacagtca atgcgcaacc
     8461 ctgaagagca aatgccataa gtccttcggt ggtcaggttg acccgatttt cctgccccca
     8521 acagcggatc ttggcaggtc gagcagatcc gttatacgac gatattgctt taaatcctta
     8581 gacgcgccgc tgagcggaag gtttacttgg catagctcga aatagcttgt ggttcagccg
     8641 cttaggtatc ttaagc
//
LOCUS       DASUVS010000168        15560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1595989, whole genome shotgun sequence.
ACCESSION   DASUVS010000168 DASUVS010000000
VERSION     DASUVS010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 15560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15560
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595989"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3470)
                     /gene="mfd"
                     /locus_tag="VF182_09325"
     CDS             complement(<1..3470)
                     /gene="mfd"
                     /locus_tag="VF182_09325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX6437319.1"
                     /translation="MSLSQLEETLHRFLNGRWAGAKRLQGLQGGALAYVLGLAAAQNK
                     RPMLVISASAQDAENLHGDLSFFIGEQPALAPSRKRLHLFPSWEVLPFEKLSPHPDNL
                     AGRLEGLYRLIEEPAPIIISTPAALMQKIIPREALKRSYLYIVAGQDLSREMLTEHLA
                     QWGFQNVPLVEEYGDFSVRGGIIDLFSPGYQLPIRLEFDGDRLESIREFNPSSQRSEN
                     VLEDLLLLPVKEFSLKRVGIDQIVRKLEQRGIELDIDRSEKNLLLESIKEGIPFPGIE
                     FLLPYFYPELTSVFSYLPSDTLVCLAGADRVEAEIERFAKLVWDRNAKAKEEGRLVAP
                     VEQLYLNEHEWRAAIEPLAQVNCEVLTIMAAAERLQDSTLTIDSFLTNDIQHENLIKH
                     GKEQSLAPLVERLTKWEGQKIIFVAPTQGDAGRLRELLGHYEIDVPIIDQPIYGLLNS
                     SKLSRAIVLGHLNQGFRLPGAALIVITFDEIFGTRKRHSTTVAKKHPSHFLTSLSELK
                     QDDFVVHLDHGIGVYRGLKFLTVAGVQGEFLHLEYEAGDRLYLPVDRINVVQKYIGGD
                     GAQPSLDKLGGTSWERVKAKARKSIFAMAEELIQLYAVREAREGNAFAPPDALYREFE
                     AGFEFEETHDQQRAIDESLADMQRRKPMDRLVCGDVGFGKTEVAMRAAFLAVEGGKQV
                     AVLAPTTILAQQHLQTFRHRFRNHPVRIEMVSRFLTNKEIEQVLKDGAKGTVDIVIGT
                     HRLLQKDVEFKELGLVIIDEEHRFGVAHKERLKKLRQMVDVMSLTATPIPRTLHMSII
                     GIRDLSIIETPPVDRLAIQTYLTRYDERVIRDAILRELERGGQVFFLHNRVETIDRIA
                     MKLADLVPEAKMSVAHGQMKPKDLEKVMLDFLENKTQILVCSAIIESGLDFPNANTII
                     INRADRFGLAQLYQLRGRVGRSHRHAYAYLLIPGEQAITPDAEKRLRALQEIDGLGGG
                     FKLALHDLEIRGAGNLLGQQQSGNITAVGFELYTEMMENAVRELRGEEVMPDIEPEIR
                     LGISAYFPDDYIPDVNQRLFFYKRLASLRNPLELEEIKQEIRDRFGPQGAAVDNLFLV
                     MNLRRVLKQFLVQQISASEEKVFLLFHPQSPVKIEKLLELINKPKSRFRLAPDGRLSF
                     SPKNQEWESL"
     gene            complement(3471..3845)
                     /locus_tag="VF182_09330"
     CDS             complement(3471..3845)
                     /locus_tag="VF182_09330"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX6437320.1"
                     /translation="MFDPMDARLREGVNLFNQGRFFEAHETWEMLYQRAAINDKAFLE
                     GLIQLAAACRLIEDFGEIKGPVKLIYQALIRFENYQPTYLDVKVADLAAAMEVWAKEI
                     EASGTRGRQPAPTIAVRRFGLF"
     gene            complement(3993..5240)
                     /locus_tag="VF182_09335"
     CDS             complement(3993..5240)
                     /locus_tag="VF182_09335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6437321.1"
                     /translation="MRFSPTPWLVAALAFVVLGFSRGLHSSFGVFNVALLDSFNWSRG
                     ATAGIFSVVLAIDAMLSPMAGYLLDRYGAKKVVLAGCVTLALGLFLSSQVSELWHLYI
                     CFGLITAFGFTFVGMVPHVFLISEWFSSNRASAIGVVYAGSGVGIMILAPLSAWLIAT
                     HGWARALEIYAAVVLISLLPLVWIFYQHGPFGEKLRQHGQKRTEQNQWTAKLALKSLQ
                     FWLLFFARVCAAAGTTVIVTHQVAHVVEVGYTQLLAASIFGFAGIISSFGRVVFGFIA
                     DRLSRQAAYTLNILMTLIGVGALMILRDARQSWLLYVYVVCFGIGFGSRAVIFSALTA
                     DIFSGKGFGSILGYSTVAVGVGGALGSWLGGVYYDWTGSYLVSFGLSALVLSLSDICI
                     WLAAVPSVANYDKRLWGETPAAH"
     gene            complement(5524..6507)
                     /locus_tag="VF182_09340"
     CDS             complement(5524..6507)
                     /locus_tag="VF182_09340"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437322.1"
                     /translation="MVSVTVRQLAWLVSSLILFAAGSAGGAENMRVAHPSLSSSVICL
                     LIANKEGYFREEGINVEFLSIRGEIAIRTALAGEVDFFTNAGSAMAAAARNVPVKILA
                     VFQDRPGWDLIAQPNIKSIAQLRGSTIAIMSPEGSLAVVTREILKKNGMDPAKDANLV
                     VMGGDDVRLPAMKAKAIQASLFNAAASVRAQKDGFVKLASASEYATTLQGGLATTEEK
                     IRQHPARMAKFIRGSLKGLQFFINKRDASVKHMMQFLKTNDHEMATAIYDIEAKLIVR
                     DGASDDKVLQPMIDEMKRATKTQRDMKVTDVFDFSMVRKVNEELQISGWRP"
     gene            6766..8235
                     /locus_tag="VF182_09345"
     CDS             6766..8235
                     /locus_tag="VF182_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX6437323.1"
                     /translation="MSNSILEFDVAIIGGGGAGIPAAIEVGRAGGTAAVLEQAAECGG
                     TAAISGGGCCIVGTPLQKSQGIEDTPDLAFEDWIKWGGGSADEVWARYYLEHTLHDLY
                     YWAENCGAKWVDMKFQEGNRVLRWHRPENNGLGLMTALIKTAESMPNIRIMTATGAGK
                     LLTKNSRVIGVTAINNNETVEIFSKAVVVATGGFNSNLDMVLEARPEFRKFRVLEGSG
                     FGAKGIGHKMIKELGGYFTHMDHIWFYVYATPDYRDPQQRRGLVFRMTPGYIWFNQQG
                     KRFHNESITGGASATPALLAQNPPHAWAILDHPMTAKMDVADPYYRRGDVIDHAKVQE
                     LLDNSPYIRKADSLEELGKKIEVDLASFLSEIKNYNDVFDRGLETEPKFGKSLKLSKK
                     FDTPPYYAIQLFPLARKNFGGVKTDLCCRVLDKHFEPIPGLYAAGEVSGMAGGHINGR
                     NGLEGTMLGPSVFSGRVAGAWAAHEAGFGPGFIGKPNRP"
     gene            complement(8462..9121)
                     /locus_tag="VF182_09350"
     CDS             complement(8462..9121)
                     /locus_tag="VF182_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437324.1"
                     /translation="MIKRAAVLFTWMAATFLFASCVSIEKSYPDKRYFVLDITADAAP
                     ENPGETGTLQIGSARVSPRYADRSFVYRRSETRFESDFYNQFLISPGVLLAEELRQGL
                     SQSGIFKFVVGSANAVPPTHTLEIIVNTLYGDFRDLRAPKAVMEIEFFLSQEGSPNGI
                     VFHKRYRSAISLEARNPEALVQGWNRALEAISGSLVGDLKASNLQLEKRSTSQQPTVS
                     G"
     gene            complement(9118..10176)
                     /locus_tag="VF182_09355"
     CDS             complement(9118..10176)
                     /locus_tag="VF182_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX6437325.1"
                     /translation="MRKFGYFKIGLFVISAILIGISGVIALGLGSIFQKKNLAETYIE
                     ESVQGLDVGSPVKFRGVSIGRVEQITLTSAEYLTRRRYVLVRFDISPNIFGFPGGDKS
                     DKALEDELQYGFRVRLAAQGLTGVAYIEADYLDPDRNLPLEIDWRPKYPYIPSAPSRI
                     TQLSESVERILRNVEELDIPQLTASVEKSLAAMTKLAEGANLEQIARQANALLSEIRD
                     TNRQLGVLVHAPELKSALADTAAAAGTARRLIENAEKPVSQLLTDLPGVSESISHVAL
                     RLEAVTADLPQTSSQFQQTLRQLNRLIVTQQEQIQSTLESLRSVTEDIQEFANESRKY
                     PSQILFGAPPPRSEVFEQ"
     gene            complement(10212..10979)
                     /locus_tag="VF182_09360"
     CDS             complement(10212..10979)
                     /locus_tag="VF182_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6437326.1"
                     /translation="MQQEQREIIRVEDLTVAYNGQVILDHVSFTVAAGEVFVILGGSG
                     SGKSTLLKNMIGLYKPATGSIFIDGENITGAEGEDRLAILRKIGVMYQSSALFGSMTL
                     LENVSLPLEEFTALPAEAIKLIALIKLKQVGLESFGNHMPSELSGGMQKRAAIARAMA
                     LDPRVLFLDEPSAGLDPITSAELDELILRLAKTLKITFVIVSHELSSIFTIADRVIML
                     DKQTKGIVATGNPHELRDRSDNPRVRQFFLRRPPSRS"
     gene            complement(10989..12125)
                     /locus_tag="VF182_09365"
     CDS             complement(10989..12125)
                     /locus_tag="VF182_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HEX6437327.1"
                     /translation="MMDFPGHIELHTRRTEANVLEIIIEGRLDSFTTSSIWRKASDTV
                     AGAKTSTVLLNASNIDYCDGSGVALLIEMRQQQLRSGGRFEIRGLQPELQALVEEWRT
                     ESFADGGYQSAGRSSAIEEIGRSIVEICDDIYSLLIFVGELAAALTRAVLKPRVARWR
                     DTLRIAESVGVNALPIVALIGFLMGLIMAFQAAIPLGQFGAQIFVANLIGLAILRELG
                     PLMTAIVLAGRSGSAFAAELGTMKVREELDALKTMGLDPVPFLVAPRVIAAVLMTPLL
                     TIFADLVGLIGGAVVLLSLGFPLITFWNQVQLAVGHGDLLGGLAKSFVFGILVAAIGC
                     LRGLQTQTGAAAVGESTTRAVVSGIILIVIADGLFSVIYYYLGI"
     gene            complement(12493..13548)
                     /locus_tag="VF182_09370"
     CDS             complement(12493..13548)
                     /locus_tag="VF182_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX6437328.1"
                     /translation="MKLPSPLRGRGSGSNPKNRFEPIERIREPSTDGDEISSPQTQFF
                     RDESKSLIAYNNSPDVGFNASINPYRGCEHGCVYCYARPTHEYLGFSSGLDFETKIMV
                     KEDAPELLRSELSTPNWKPQVVALSGNTDCYQPVEKKKQLTRRCLEVFLDFRNPVVII
                     TKNHLVTRDIDLLAELAKYQCIGVTISITTLDTKLASLMEPRASTPERRLAAIRALSQ
                     AGVPVGYLQAPMIPALTDSEAPAIALAAAKAGAKFCGYVTLRLPFAVKLLFEHWLEQH
                     YPGKKEKVLNRIRSMRAGKLNDPNFKSRMRGEGIFADQMAELFQLAQKKAGIWDRWPQ
                     FSLEYFRRTSDNQLRLF"
     gene            complement(13553..13774)
                     /locus_tag="VF182_09375"
     CDS             complement(13553..13774)
                     /locus_tag="VF182_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437329.1"
                     /translation="MDVNALPSLKQNIRQLKKEKAEILTSSGDSAKVKRIRRKIKLLK
                     RETRELAAQKKKATVKASAEKTTATAASE"
     gene            13832..14503
                     /gene="nth"
                     /locus_tag="VF182_09380"
     CDS             13832..14503
                     /gene="nth"
                     /locus_tag="VF182_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX6437330.1"
                     /translation="MPVPTRNAFAGTKTRIKKIIERLAKIHPDAKLDLDFSSALELLI
                     ALILAAQARDDLIRKITPGLFKKYLTAKDYAKTSLPKLQRDIGRINFYRNKSRSIHNC
                     CSKLVERFGGEVPDNLDDLLSLPGVGRKTANILLGNAFGQQTIGVDTHVMRLSQRLGL
                     TEETDPDKIEADLIRVVAPAQRVRFCHLLQYHGRRICIAKKPKCAECIIREFCPYQQK
                     CLGMK"
     gene            complement(14613..14728)
                     /gene="rrf"
                     /locus_tag="VF182_09385"
     rRNA            complement(14613..14728)
                     /gene="rrf"
                     /locus_tag="VF182_09385"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(14836..>15560)
                     /locus_tag="VF182_09390"
     rRNA            complement(14836..>15560)
                     /locus_tag="VF182_09390"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         3826 a         4197 c         3950 g         3587 t
ORIGIN      
        1 atcaatgatt cccattcctg gtttttcggt gaaaacgaaa gacgaccgtc cggcgctagc
       61 ctgaaacgac tcttgggttt gtttattagt tcgagcagtt tttcgatctt caccggcgat
      121 tgcggatgaa acagtaagaa aaccttctcc tcggatgcgc tgatctgctg caccagaaat
      181 tgtttgagca cgcgccttag gttcataacc aaaaaaagat tgtccacggc tgcgccctga
      241 ggaccaaacc ggtcacggat ctcttgcttg atctcctcta actcaagcgg attacgcagg
      301 ctcgccagcc gcttataaaa aaacaaacgc tgattcacat cgggaatgta atcatctggg
      361 aagtaagcgg atatacctaa acgaatttcc ggctcgatgt cgggcatcac ttcttcgcct
      421 ctgagttcac gcacggcatt ttccatcatt tcagtgtaaa gctcaaatcc taccgccgtt
      481 atattgccgg actgctgctg tcctaaaaga ttgccggcgc cgcgtatttc cagatcgtgc
      541 agcgccaatt tgaatccgcc acccaaccca tcgatctctt gcagcgctcg cagtctcttc
      601 tccgcgtcag gagtgatcgc ctgttcgccg ggaataagaa ggtaagcata agcatgccgg
      661 tgggagcggc cgactcgccc tcttaactgg tagagttgcg ctaaaccaaa tcgatcggcg
      721 cggttgataa tgatggtatt ggcgttggga aaatccaggc ccgactcgat gatcgccgag
      781 cagaccaata tttgcgtctt attctcgaga aaatcgagca tcactttttc cagatctttg
      841 ggtttcattt ggccatgtgc cacagacatc ttggcctcgg gaaccaaatc ggcaagcttc
      901 atggcgatac gatcgatggt ttcaactcgg ttatgtagga aaaacacctg tccgccacgc
      961 tcgagttcgc gtaaaatcgc atcccgaatc acccgctcgt cgtagcgagt cagataggtc
     1021 tggatcgcca gacggtcaac cggtggagtt tcgataatgc tcaggtcgcg tatgccgata
     1081 attgacatat ggagcgtgcg cgggatcggc gtggccgtaa ggctcatgac gtcgaccatt
     1141 tgacgcagtt ttttaagccg ctctttgtgc gccacgccga atcgatgctc ctcatcgatg
     1201 atgacgagtc ctagttcctt gaattcaaca tctttttgca gcaaccgatg tgtaccgatg
     1261 acgatgtcga ccgttccttt cgctccgtct ttgagaacct gttcgatctc cttgttagtt
     1321 aagaaacggc tgaccatttc gatccgcacc ggatggttgc gaaagcgatg gcgaaatgtt
     1381 tgtaggtgct gctgcgccag aatcgtcgtt ggcgccagca cggccacctg cttgccacct
     1441 tcgacggcta gaaaggcggc gcgcatggcg acctccgtct taccgaaacc gacatcgccg
     1501 cataccaacc ggtccatcgg ctttcgtcgc tgcatatcgg cgagggactc gtcgatggcg
     1561 cgctgttggt cgtgggtttc ttcaaattca aaaccggctt cgaactcccg gtacaacgca
     1621 tcgggcgggg caaaagcatt gccttcacga gcttcacgca ctgcatagag ctgaatcagt
     1681 tcttccgcca tggcgaaaat cgatttgcgt gctttggcct tgacccgttc ccaggatgtg
     1741 ccgccgagct tgtcgagcga aggttgggcg ccatcgccgc ctatgtactt ctgcacgaca
     1801 ttgatgcgat cgaccggcag gtaaagccga tccccggcct catactccaa gtgcaagaac
     1861 tcgccttgga ctccggcgac tgtgagaaac ttcaggccgc gataaacgcc gatgccatga
     1921 tcaagatgga ctacgaaatc gtcctgcttc agttcgctca ggctggtgag gaaatggctt
     1981 gggtgctttt tggccaccgt cgtggaatgg cgcttgcgcg taccgaatat ctcatcgaaa
     2041 gtaattacga tcagcgccgc gcccggtaga cggaatcctt gattgagatg tcccagaacg
     2101 attgcccggg agagcttaga cgagttcagc agtccataaa tcggttgatc gattatcggg
     2161 acatcgattt cgtaatgacc caatagctct cgaagccgac cagcatcgcc ctgggttgga
     2221 gctacgaaga tgattttttg cccctcccat ttagtgagcc gttcgacaag cggcgccaaa
     2281 gactgctctt tgccgtgctt gatgagattt tcgtgctgga tgtcgttggt gagaaacgaa
     2341 tcaatggtca aggtcgagtc ctgcaagcgc tcggccgcag ccatgatcgt caagacttcg
     2401 caattcactt gagctaaagg ttcgatggcg gcacgccatt catgctcgtt gagatagagc
     2461 tgttctaccg gtgccaccaa acggccttct tcttttgctt tcgcattgcg atcccagacg
     2521 agcttagcga atcgctcgat ctctgcttcg acccgatccg cccccgccag gcaaaccaaa
     2581 gtatccgatg gtaggtaaga gaacaccgag gtcagctccg gataaaaata tggcagcaag
     2641 aactcgatgc cgggaaaagg aatgccctcc ttgatcgatt ccagcaaaag attcttttcg
     2701 ctccggtcga tatccaactc gatcccgcgc tgttccagtt tcctgacgat ttgatcgatg
     2761 ccgactcgct tgagagaaaa ttccttcacc ggcaacagca gcagatcctc caaaacgttt
     2821 tccgagcgtt ggctggatgg attgaattcg cggatggatt ccaagcgatc gccatcgaat
     2881 tccaggcgga tcggcaactg ataacccgga gaaaagagat cgatgatgcc gccacggacg
     2941 ctgaaatctc cgtattcttc gactagggga acgttctgaa atccccactg cgccagatgt
     3001 tcagtcaaca tttcgcggga tagatcctgc ccggcaacaa tgtagaggta agatcgtttt
     3061 agcgcctctc gcggaataat cttctgcatc aacgccgcag gcgttgaaat tatgattggt
     3121 gccggctctt caattaacct gtagagtccc tctagtcgtc ccgccaaatt gtcgggatgg
     3181 ggagataact tctcaaaagg gagaacctcc caggacggaa atagatgaag ccttttacgt
     3241 gacggagcta aagcgggctg ctcgccgata aagaagctaa gatcgccgtg caaattttca
     3301 gcatcctgag cgctggcgga gatgacgagc attgggcgtt tgttttgtgc cgccgccaac
     3361 cccaatacgt aagccagcgc tcctccctga agaccctgaa gccgcttggc tcctgcccac
     3421 cgtccattaa gaaagcggtg cagtgtctct tccaattgtg aaaggctcat ttagaaaagc
     3481 ccgaaacgcc ggacagcgat cgttggagca ggttgtcgtc ccctggtacc gctggcttcg
     3541 atctctttgg cccagacctc catcgccgca gctaggtcgg ccactttgac atcgagatag
     3601 gtgggttgat aattctcaaa gcggatcagc gcttggtaga tcaactttac cggtccttta
     3661 atctcgccga aatcttcgat caaccggcaa gccgcagcta gttggatcag tccctcgaga
     3721 aacgctttgt cgtttatcgc ggctcgctga taaagcatct cccaggtttc atgagcctcg
     3781 aaaaaacgac cttgattgaa aagatttacc ccttcgcgta agcgcgcatc cataggatcg
     3841 aacattcaag gtaacaactt ctcgccatac cctcaaggtt gatgcagtag tagtcgctta
     3901 gctaaggctg tgagggcgct gccgggcaca agatggcacg atgcagtgaa tggcaatcgg
     3961 ttatacaatg cttcaggcac aggaaaggtg gattaatgcg cagctggagt ctctccccag
     4021 agtcgtttgt cgtagttagc cacagacggc accgcagcca accagatgca aatatccgat
     4081 aaggacagca ccaaagccga taagccaaac gaaacaagat aactgccggt ccaatcgtaa
     4141 taaacgccac ccaaccagga gcccagcgca ccgcccacac caacagccac tgtcgagtaa
     4201 ccgagaatcg aaccgaaacc tttgccggaa aagatgtccg cggtcaacgc cgagaagatg
     4261 acggctcgcg aaccgaaacc tatgccaaag cagaccacat agacataaag taaccagctt
     4321 tggcgggcat cacgcaaaat catcagagcg ccaacgccaa tcaacgtcat caagatatta
     4381 agggtatagg ctgcttgcct ggataagcga tcggcgatga agccgaaaac cacccgacca
     4441 aagctactga tgatgccggc aaaaccaaaa atggacgcgg ccagcaattg cgtatagcca
     4501 acctcgacga catgagcgac ttgatgagtc acaatgaccg tcgtacctgc cgcggcacaa
     4561 acccgcgcaa aaaaaagcag ccaaaactga agacttttta aggcgagctt tgccgtccat
     4621 tggttttgct cggtcctttt ctgcccatgc tgacgaagtt tttcgccgaa cggtccgtgc
     4681 tgataaaaaa tccacaccag aggaaggagc gatatcaaaa ctactgctgc ataaatctcc
     4741 agagcccgcg cccaaccatg agtggcgatc agccaggcgc taagtggcgc aagaatcatg
     4801 attcccacgc cggaaccggc atagacgaca cctatggccg atgcccgatt ggaggagaac
     4861 cattcggaga ttagaaagac gtgcggcacc atgccgacga aggtaaaccc aaacgcagta
     4921 atcagcccaa aacagatgta caagtgccaa agctcgctaa cctggctgct caaaaagagg
     4981 cccaatgcca gcgtgacgca cccggcaagc acaacttttt ttgctccata acggtcgaga
     5041 agataaccgg ccatcggcga caacatggcg tcgatcgcaa gcacaaccga aaagataccg
     5101 gcagtcgcgc ctcggctcca gttgaaagag tcaagcagcg caacgttgaa aactccgaac
     5161 gaggaatgca gcccgcgcga gaacccgaga acgacgaagg caagcgcagc aactaaccag
     5221 ggtgtaggag aaaaacgcat gacgttcggt gcgcctctgt tgtagcaaag tggaaggtgg
     5281 gccacaacta agaacgcgcc agccccggaa agcgcctatc acgagatctt cagttgaatc
     5341 gtaagcgtca aacagctgca gaacgcgatg ttcaactggc cagaatcatg aaattacaaa
     5401 cgcagcatag ggggaattcc tttgtcggaa attctttgtt cttcgggcca taggagaatc
     5461 gatatagagg ccacaatgat aatgccgctg caggaaaacc aagattccat tggaagggtc
     5521 gtgttatggg cgccagccgc taatctgcaa ttcttcgttc actttacgca ccatgctaaa
     5581 gtcgaagacg tcggttacct tcatgtcgcg ctgcgttttt gtcgcccgtt tcatctcatc
     5641 tatcatcggc tgcagaactt tgtcgtcgct ggcgccgtca cggacaatga gctttgcttc
     5701 aatatcgtaa atcgccgtag ccatttcatg gtcgttggtc ttgagaaatt gcatcatgtg
     5761 tttgaccgag gcatcccgct tgttgatgaa gaactgcaat cccttcagac tgccacgaat
     5821 gaacttggcc atgcgcgccg ggtgttgccg gattttctct tccgtggtcg ccagaccgcc
     5881 ctgcaacgtc gtcgcgtatt cgctggcgga ggctaactta acgaatccat ctttttgcgc
     5941 ccgaacactg gcagcagcat taaacaacga ggcttggatc gctttcgcct tcatcgccgg
     6001 cagacgaacg tcgtcgccgc ccatcacgac caaattggca tctttcgccg gatccatacc
     6061 attctttttt aaaatttccc gggtcacaac cgccaatgat ccttccggcg acattatggc
     6121 aatggtacta ccccggagtt gagcgatgga tttaatattt ggctgggcaa tcagatccca
     6181 accaggtctg tcttgaaaca ccgcaagtat cttaaccgga acatttcgcg cggctgccgc
     6241 catcgcgctg cccgcattag tgaagaaatc cacttcgccg gcgagcgccg ttctgatggc
     6301 gatctcgcca cgtatactca ggaattcgac attgattccc tcttctctga agtagccttc
     6361 tttgttggca atgagcaggc aaatgaccga tgaagaaagc gacggatggg ccacgcgcat
     6421 attttctgcg ccacccgccg aacccgcagc aaaaaggatt aaggaagaaa caagccatgc
     6481 aagctgacgt actgtaacag agaccattat cagatccctc cttgccggat gagccgaaac
     6541 agcttccctc ttcgcttata gaaagattaa aagattggca agcgagaagt tagtgtgccg
     6601 cagactgaat acatgggagg aacatttcag tgggaaagct ttacgtcact ggaagctaat
     6661 ggttctggtc acacgagatc gcaacagaaa tcgggattta ttaggatatt tgaccacaag
     6721 gcaagaacgg tgataatcgt cggcaggtat aaaaaggaga tcccgatgtc taattcaatt
     6781 ttggagttcg atgttgccat catcggcggc ggcggcgcag gcatacccgc agcgatcgaa
     6841 gttggccggg caggcggaac agccgcggtc ctggagcaag ccgcggagtg tggcggcact
     6901 gcggcgatct ccgggggcgg ctgttgcatc gtggggacgc cgctacaaaa atcgcagggc
     6961 attgaagaca cgccggatct ggcgtttgaa gactggatca aatggggcgg cgggtcggcg
     7021 gatgaggttt gggcgcgata ctatctcgaa catacgctgc acgaccttta ctactgggcc
     7081 gagaattgcg gcgccaaatg ggtcgatatg aaattccagg agggcaatcg ggtgctacgc
     7141 tggcaccggc cggagaacaa cggcctaggc ttgatgaccg cattaatcaa gactgccgag
     7201 tccatgccga atatcaggat catgacggcc accggcgcag gcaaacttct cacgaagaat
     7261 agccgtgtga ttggggtcac ggcgatcaac aacaacgaaa cggtcgaaat cttcagtaag
     7321 gccgttgtcg ttgctaccgg aggtttcaat tcgaatctcg atatggtttt agaggctcgg
     7381 cccgaattcc ggaaattcag agttctagaa ggctctggtt tcggcgcaaa aggtatcggt
     7441 cataagatga ttaaagaatt aggcggttac ttcacccaca tggaccacat ctggttttat
     7501 gtttatgcga ccccggatta tcgtgatccc caacaaagac gcggtctcgt ctttcgcatg
     7561 accccggggt acatttggtt caaccagcaa gggaaacggt tccataacga atcgattacc
     7621 ggcggcgctt cggctactcc cgccctttta gcgcaaaacc cgccgcacgc atgggcgatc
     7681 ctcgatcatc caatgacagc aaaaatggat gtggccgatc cctattatcg ccgcggcgat
     7741 gtgatcgacc acgccaaagt ccaagaactt ctggacaatt cgccgtatat tcgcaaggcc
     7801 gatagcctgg aagagttagg caagaagatc gaagtcgatt tagcttcatt tctttccgaa
     7861 atcaaaaact acaacgatgt ctttgatcgc gggctggaaa ctgaaccaaa gttcggcaaa
     7921 tctttgaagc tttcaaagaa attcgacact ccgccgtact atgcgattca gctgtttccg
     7981 ctcgcacgga aaaatttcgg cggggtcaaa acggatttgt gctgccgcgt gctggacaag
     8041 catttcgagc cgatccccgg cttgtatgcg gcaggcgaag tatccggcat ggcaggcggt
     8101 catatcaatg ggcgaaatgg attggaagga accatgctcg gaccgtcggt ttttagcgga
     8161 cgagtagcag gcgcttgggc ggcacatgaa gcaggcttcg gtcctggatt tattggcaag
     8221 cccaaccggc cgtaacgtcg ctcttttgcg ggtgagccac gatcgcgact tggaactact
     8281 gcgtcatcat ttagcgcccg cacctgaggc ggaatcttgg ttctcgagtg acattgtcaa
     8341 gcgcgccgct ggagagagtg ccaatgcgcg gagcatgggt cgctcacgcc atgctctaca
     8401 gtgcagatgt gcggagtttc ctaccctctt gaatctcagc gccaccacgg cgagcaatcg
     8461 ctcaccctga aactgttggc tgctgcgaag tagatcgttt ttccaattgc agattagacg
     8521 cttttaagtc accgacgaga gagcccgaaa ttgcctcgag agcccgattc cagccttgca
     8581 ccagcgcctc cggattacgt gcttcgagcg aaatagcact gcgatagcgt ttgtgaaaaa
     8641 caattccatt gggtgatccc tcttgactga gaaaaaactc gatctccatc actgctttcg
     8701 gcgctctgag atcgcgaaag tcgccataga gagtattcac gataatctcc agcgtatgcg
     8761 ttggcggtac cgcgttggca gaaccgacga cgaatttaaa gattcccgac tgtgacaacc
     8821 cttggcgcag ctcttcggca agtagcacgc cgggagatat cagaaattgg ttgtaaaaat
     8881 ccgattcgaa tcgagtctcg cttctccgat agacgaaact tctgtccgca taccggggcg
     8941 atacacgtgc ggatccgatt tgcagagtgc cggtttctcc tggattttcg ggcgctgcgt
     9001 cggcagtgat atcgagcaca aaataacgtt tatcaggata gcttttttca atgctgacac
     9061 agctggcaaa gagaaaagtt gccgccatcc atgtgaacaa cacagcagcc cgctttatca
     9121 ttgctcgaag acctctgaac gaggtggcgg cgcgccgaaa agaatctgcg atggatattt
     9181 ccgagactcg ttcgcaaatt cttggatatc ttcggttact gaccgtagac tttccaatgt
     9241 gctttgaatt tgctcctgct gtgtgacaat gagccggtta agttgccgca gtgtctgctg
     9301 aaattggctg cttgtctgcg gcagatcggc tgtgactgcc tctagccgca gcgctacatg
     9361 actgatgctt tctgatacgc cgggtaggtc ggtaagcagc tgacttaccg gtttttcggc
     9421 gttttctatt agtcttcgcg ccgtgccggc ggccgctgcg gtatctgcca aggcactctt
     9481 cagttcgggt gcatgcacca gcaccccgag ctgacgattc gtatctcgga tttcgctcag
     9541 cagggcattg gcttgcctgg caatttgttc cagattggcc ccctccgcaa gctttgtcat
     9601 ggcggcaagc gacttttcga cgctagcggt tagttgagga atatcgagtt cctcaacgtt
     9661 gcgcagaatg cgttctaccg attcgctcag ctgagttatc ctgctagggg ccgaaggaat
     9721 atagggatac ttcgggcgcc aatcgatttc caacggtaga ttgcggtcag ggtccaaata
     9781 gtccgcttct atgtaggcga ccccggttaa gccctgagcc gctaatctca cgcgaaatcc
     9841 gtattgaagc tcatcctcga gcgccttatc cgatttatct ccgccaggaa aaccaaaaat
     9901 attcggtgag atatcaaaac gcaccagaac gtagcggcgc cgagtgagat attctgcgct
     9961 ggtcaacgta atctgctcga ctctgccgat ggaaacaccg cggaatttca ctggcgagcc
    10021 aacgtccaat ccctggacgg attcctcgat gtaagtttcg gcaaggttct tcttctggaa
    10081 aatcgaaccg agacccaaag cgatcacacc gctgattccg atcagtatgg cgctaattac
    10141 aaatagacca atcttgaaat atccgaattt tctcatcctt ggtgtgactt gagatttagc
    10201 ttgcgccgga ctcaagacct cgaaggcggc cggcgcaaga agaattgacg tactcgggga
    10261 ttatcgctgc gatcgcgcag ctcgtgtgga tttcccgtcg ccacgatccc tttggtctgt
    10321 ttatccaaca taataactcg atccgcaatc gtaaagatac tcgacagctc atgactgacg
    10381 atcacaaatg ttatcttcag agtttttgct aggcgtaaaa tcagctcatc cagttcggcg
    10441 gaagtgatgg ggtcgagacc ggctgatggc tcgtcgagaa atagcacccg cgggtcaagc
    10501 gccatggcgc gcgcaattgc cgcccgcttc tgcatgccac cgcttaactc tgacggcata
    10561 tggttgccga aactttcgag accgacctgt ttcagtttta tcaaagctat aagtttgatc
    10621 gcctcggcgg gcagtgctgt gaattcttca agcggcagag aaacgttttc gagaagcgtc
    10681 atcgaaccga acaatgcact gctctgatac ataacaccga tctttcttag tatggccagt
    10741 cgatcttcac cttcagcgcc ggtaatattt tctccatcga tgaaaatgct tccagtggct
    10801 ggtttgtaga ggcctatcat attttttaat agcgtgcttt tacccgagcc ggagccgcca
    10861 agtatgacaa acacctcacc ggcggccact gtaaaactca catgatcgag gatcacttgg
    10921 ccgttgtacg ccacggtaag atcttcaacc cgaataattt cccgctgctc ttgttgcacg
    10981 cctgcccgct aaataccgag gtaataataa attaccgaaa aaagaccatc ggcgattacg
    11041 attaagatga tcccgctcac cacagcacgg gtcgtcgatt cgcccaccgc ggcggcaccc
    11101 gtttgggtct gcagaccgcg caggcagcca attgccgcca cgagaatgcc gaaaacgaat
    11161 gatttagcca gcccgccgag cagatcgcca tgaccaactg ccagttggac ctgattccaa
    11221 aaagtgatca aaggaaaccc aagcgaaagc aatactaccg cgcctcctat cagcccgacg
    11281 aggtcggcaa agatagtgag tagcggtgtc atgagaactg cagcgatcac tcgcggcgca
    11341 accagaaaag gcacgggatc caatcccatc gttttcaagg cgtcgagttc ttcacgaact
    11401 ttcatcgtcc ccaattccgc tgcaaacgcc gaacccgaac gtcctgccaa gacaatggcg
    11461 gtcatcagcg ggccaagctc gcgcagaatc gcgagaccga ttagattcgc gacaaatatc
    11521 tgcgcgccga actggcccag cggtatagca gcctggaaag ccataattag gcccatgaga
    11581 aatcctatca gcgcgacaat gggcagagca ttgactccga ccgactcggc gatacgtaag
    11641 gtatctcgcc agcgagcgac gcgcggcttg agaacggcac gtgtcagagc cgccgccagt
    11701 tcgccgacga atatcaacaa actgtagata tcgtcgcaga tctcaacgat cgagcggcca
    11761 atctcttcga tcgccgaaga tcttcctgca ctctgatatc ccccgtcagc aaaactttcc
    11821 gttcgccact cttcgacgag cgcctggagc tccggctgta gcccgcgaat ttcaaagcgt
    11881 cctccggacc ggagttgctg ctgacgcatt tcaattaaca aagcaacgcc cgaaccgtcg
    11941 caataatcaa tgttcgaggc attcaacaaa accgttgaag tcttggcgcc ggcaacggta
    12001 tcgcttgctt tgcgccagat gctgctggtc gtaaacgagt ccagccggcc ctcgataatt
    12061 atttcgagta cattagcttc ggttcttcgt gtgtgaagct cgatgtggcc cgggaaatcc
    12121 atcatgaggt taaagcagac ttctattatg ctgcgtctgt tagaagcgtc aaccgtcgac
    12181 gtgcgaaaat cacagccttt gtgcttgaca ttcagaaagg cgttcagttt ctttagtaag
    12241 gcttggccta gagatcaggc cctagtagag tacgtcatga agtactctcc aatttggatt
    12301 atctggctgg agcgaagagc cgggaacgat caagctttgg caagtagcat ctggaaatga
    12361 ttcagaacaa ctcggagcgt atagagagaa caaaaaacgt atgattaacg tcgaagcctt
    12421 agcctaagct tttttctatt tcagtgaatt cgcgcgtgga tgtttgaggt gcatcgggaa
    12481 gaatcgcatc tgttagaaaa ggcggagctg attatcgctt gtacggcgaa aatattctaa
    12541 cgaaaattgc ggccaacgat cccaaatgcc tgctttcttt tgagcaagct gaaacagctc
    12601 ggccatctga tcggcaaata taccctcgcc ccgcatgcga ctcttgaagt ttggatcatt
    12661 gagcttgccg gctcgcattg atctaatgcg atttagaacc ttttctttct tgccgggata
    12721 gtgctgctcc aaccaatgtt cgaacaataa cttgacagca aacggcaacc gcaatgtcac
    12781 ataaccgcaa aacttcgcac ccgctttagc agctgccaga gcgatcgcgg gcgcctctga
    12841 atcggtgaga gctggaatca taggcgcctg aagataaccc accggcacgc ccgcttggga
    12901 tagtgcccgg atggccgcca accggcgctc gggagttgat gcccgcggct ccatcaaaga
    12961 tgccagttta gtgtccaagg tggtaatcga aatcgtcacc ccgatacatt ggtactttgc
    13021 aagttctgct agaagatcga tgtcgcgcgt taccaggtgg ttctttgtga tgattaccac
    13081 gggattacga aaatcgagga acacttccaa gcatcgcctc gttagttgct tcttcttctc
    13141 caccggctga taacagtccg tattgccgct cagcgcgaca acctgcggct tccagttagg
    13201 ggtcgataat tcactgcgca atagctcggg cgcgtcctct ttcaccatga ttttggtttc
    13261 gaaatctaac cctgaagaaa aaccgaggta ctcgtgtgtc ggacgcgcat agcagtaaac
    13321 gcatccgtgc tcgcaacccc gatagggatt gatgctagcg ttgaatccga catccgggct
    13381 attgttgtag gcgatcagtg atttggattc atcccggaag aattgtgttt gaggtgagga
    13441 gatttcatcg ccatcggttg acggctctcg aatccgctca atcggttcga agcggttctt
    13501 tggattgctt ccagatcctc tgccgcgcag cggggacggc aatttcatgc tcttattcgc
    13561 tcgctgcggt tgcagtggtc ttctcagccg aggctttgac cgtcgctttc ttcttctgcg
    13621 ctgccagctc gcgcgtctcc cgcttgagca gcttgatttt ccggcgaatc cgcttcacct
    13681 ttgcgctatc gcctgacgaa gttaggattt cagctttttc tttcttcagt tgccgaatgt
    13741 tttgtttaag acttggcaat gcgttcacat ccatatatga atttcctcct ttgcgcttac
    13801 gttagctagc acaattgcgg gacaaaccaa aatgccagta cctaccagaa acgcgttcgc
    13861 gggaaccaaa acaaggatca agaaaatcat tgagcgtttg gcaaagattc accccgatgc
    13921 gaagctagac ttggacttca gcagtgccct ggaattgttg atcgccttga tcttagcagc
    13981 ccaggcacga gacgacctga ttcgaaaaat aactccaggc ttgtttaaaa aatatctaac
    14041 cgccaaagac tacgcgaaga catcactacc taaattgcaa cgagacatcg gcaggatcaa
    14101 tttctaccgc aacaaatcgc gatcgattca caactgctgc agcaaattag tcgaacgctt
    14161 cgggggcgag gtgccggaca acttagatga tttactttcg ctccccggag ttggacgcaa
    14221 aaccgccaat atcctgctcg ggaatgcttt tggccaacag acaattgggg ttgacactca
    14281 tgttatgcgt ctttcgcaac ggttaggatt gacggaagag actgatcccg acaaaatcga
    14341 agctgatctc atacgcgtcg tcgccccagc gcaacgcgtt cgcttctgcc acctgcttca
    14401 ataccacggc agacgaattt gcatagcgaa gaaaccgaaa tgtgccgagt gcataattag
    14461 agaattctgc ccgtatcagc aaaaatgctt gggaatgaaa tagattaatc agcaatattc
    14521 tctggggcta atgtcgacga atatctttat catgtacgtc gcccacaaac aaagaagccc
    14581 caacatcttg gttggggctt ctttgaacat aaatccggcg gcgtcctact cttccataga
    14641 gcaactctat agtaccatcg gcgctggagg gcttaacggc cgagttcggg atgggatcgg
    14701 gtgtgacacc tccgctatcg ccaccggaaa cctttttaaa ccgttcaatg ttcctgactt
    14761 gaaactgttg aatgtaattc catcacaact gaatacgggc acaaacgaat tacctgctga
    14821 gtcgcattgg taaatatggt caagccgcac gaccaattag taccggttag cttcaatcat
    14881 tactgatctt ccacatccgg cctatcaacc tcctagtcta gaaggggtct ttagaggcct
    14941 aacggcctgg gataccttat ctcggagggg gcttcccgct tatatgcttt cagcggttat
    15001 cccgtccaca catagctacc cggcaatgcg gctggcgcca caaccggaac accagcggtg
    15061 tgttcttccc ggtcctctcg tactagggaa aactcccctc aagtatcctg cgcccacggc
    15121 ggatagggac caaactgtct cacgacgttt tgaacccagc tcgcgtgcca ctttaatcgg
    15181 cgaacagccg aacccttggg acctacttca gccccaggat gtgacgagcc gacatcgagg
    15241 tgccaaacac ccccgtcgat gtgaactctt ggggggtatc agcctgttat ccccggcgta
    15301 ccttttatcc gttgagcgat ggcccttcca cgcggaacca ccggatcact aaagcctggt
    15361 ttcccacctg ctccacttgt aggtgtcgca gtcaagctcg cttatgcttt tgcactctcc
    15421 ggctgatttc caaacagcct gagcgaacct tcgcgcgcct ccgttacagt ttaggaggcg
    15481 accgccccag tcaaactacc cacctagcag tgtccctgac ctggatgaca gatcgaggtt
    15541 agaacgccgg agtgttaagg
//
LOCUS       DASUVS010000169         6919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_939210, whole genome shotgun sequence.
ACCESSION   DASUVS010000169 DASUVS010000000
VERSION     DASUVS010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6919
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939210"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(472..1740)
                     /gene="serS"
                     /locus_tag="VF182_09395"
     CDS             complement(472..1740)
                     /gene="serS"
                     /locus_tag="VF182_09395"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX6437331.1"
                     /translation="MLDIRLLRDDLEQVKRRMATRGIVIDWEEFVSLDRERRDVLSRL
                     EKLKEQKNRRSGEIGIIKKTGADATGLMREVEELSKQVRDLETPLAEVEGRFENFMLT
                     VPNLPNSDVKVGKDSDDNREVRRWGAPPEFDFPARNHWEIGEELDILDFQRAAKLAGA
                     RFVIYKGAAARLERALINFMLDLHTVEHGYKEIIPPALANRAAVTGTGNLPKFEEDLF
                     RIETPDYFLIPTAEVPLTNVHREEILERDALPIKYTACTPCFRREAGSYGKDVRGLIR
                     QHQFNKVEMVKLCEPEFSYDELEAMVENAEEVLKRLELAYRVVELCTGDMGFAAAKTY
                     DIEVWLPGQGTYREISSCSNCEDFQARRAQIRFRKDKGKLTLVHTLNGSGLAVGRTVV
                     AVLENYQQKDGSVAIPQVLQPYMHGLDRIG"
     gene            complement(1750..2169)
                     /locus_tag="VF182_09400"
     CDS             complement(1750..2169)
                     /locus_tag="VF182_09400"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX6437332.1"
                     /translation="MRIRARNGSRFAVRQPVFLNRAKIVLGKEGERIAELFLKSKGYK
                     LVERNYRCAAGELDLIMLDRRVIVFVEVKTRKGVGYGTPLEAVEVRKQQRMIYAAQFF
                     LTAKKLNQREARFDVVGICWPGAQPRVEHIENAFELI"
     gene            complement(2112..2843)
                     /locus_tag="VF182_09405"
     CDS             complement(2112..2843)
                     /locus_tag="VF182_09405"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020586845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX6437333.1"
                     /translation="MGGKQYTLSEDFDRPLMQGAIERQSYSRGFSCVAGVDEVGRGPL
                     AGPVVAAAVVLPMGFTHADIKDSKMLTTEQRERLLPTIRESAVCWAVGVVEVEEIDRI
                     NILEASLLAMAKALRALRPVPDCLLIDGNQMIPAEFFSVNTLFKKPPVQRTIIKGDQL
                     CVSIAAASILAKVSRDTMMLELDKSYPEYGFAHHKGYSCITHMEALRRFGPSPIHRRS
                     FRPVRAMCALELETDLALPFVNPCS"
     gene            3015..3302
                     /gene="gatC"
                     /locus_tag="VF182_09410"
     CDS             3015..3302
                     /gene="gatC"
                     /locus_tag="VF182_09410"
                     /inference="COORDINATES: protein motif:HMM:NF014716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX6437334.1"
                     /translation="MRLNSQDVARVAQLARLRLLPEELNQLTIELQSILQYMDKLREI
                     DTSEIEPFTQAVDSSNAFRDDLVTNNPNTDAILANAPATAKTFFKVPKIIE"
     gene            3299..4765
                     /gene="gatA"
                     /locus_tag="VF182_09415"
     CDS             3299..4765
                     /gene="gatA"
                     /locus_tag="VF182_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX6437335.1"
                     /translation="MSLFSLTIHEASEKLRKREVSSQELTEAIFQRLAETDGLIRGYI
                     TLCRDSAIAQAKEADERLKREASASPLLGIPVALKDNLLTQGLRTTCASKILQNFIPP
                     YDATVVTKVRSAGAVITGKTNLDEFAMGSSAENSAFFPTRNPWHLERVPGGSSGGSAA
                     SVAADQCIAALGTDTGGSIRQPAAFCGIVGLKPTYGRVSRYGVIAFASSMDQVGPMTK
                     DVRDCALLLEAISGHDPADSTSVDFPVPSYSKSLTRDITGLRLGIPKEYFSSGLQPAI
                     EQALVIAIRQLESDGAIIEEISLPHTDYAVAVYYIIATAEASSNLARYDGMRYGQRAP
                     GGDLTETYTLSRAKGLGPEVKRRIMLGTYALSAGYYDEYYMKAQKVRTLVRRDFETAF
                     KHCDAIITPTSPSTAFKMGEKTQNPLQMYLSDICTISINLAGVPAISVPCGLDGEGLP
                     IGMQVIGKHFDEPTILRVAYAYEQQTDWHRKKPAFSDA"
     gene            4762..6201
                     /gene="gatB"
                     /locus_tag="VF182_09420"
     CDS             4762..6201
                     /gene="gatB"
                     /locus_tag="VF182_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX6437336.1"
                     /translation="MNYETIIGLEVHAQLLSDSKIFCGCSTRFGAAPNHNTCPVCAGF
                     PGALPVLNKKVVEFALRAGLAMHCKIAGSSRLARKNYFYPDLPKGYQISQYELPICSN
                     GYIDVDVGGATKRVRLIRIHIEEDAGKNIHDAYEDSSLIDLNRAGVPLLEIVSEPDIR
                     NAGEAVSYLKRLRAILRYLEVCDGNMEEGSFRCDANISLRRIGTGSLGTKVEIKNLNS
                     FKAIEKALEYETERQTDTLSTGGDLVQETRLWDDNNEQTRSMRSKESAHDYRYFPDPD
                     LLPINLDQAWTQEVCSNLPELPDARKVRFMSQYGLSAYDAELLTGRRDVADYLEAAIN
                     ICPNAKAVSNWIMGDLFRVLKERKLDEQLRIDNWPIQPTQLGDLVQLIDDGKISGKIA
                     KLIFEALLDTNQSPQVIMTQRGLEQVSDPASLERSIDQVLITHAQQVAAYRGGNDKIF
                     GFLVGQVMKASQGKANPQKVNELLKSKLS"
     gene            complement(6250..>6919)
                     /locus_tag="VF182_09425"
     CDS             complement(6250..>6919)
                     /locus_tag="VF182_09425"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX6437337.1"
                     /translation="KLVELAEKRDQLESASNSIDSQLRASISELRPTEFSQVDVGFHT
                     PPVPGSTETQLSPTQQVKPGSESERPDEVASVPKMDLRSADTKATAPVLIKPSKQQNA
                     AELDTFEQQPVLGHAKRIEDVPEMKSLVRPVPKALEGYIIQMSFQDHSEARRWADTFE
                     QRGYAVSITEAGTADSLRVRIGNFRLRDDAERQLKSIREGGLVGVILNLPQPYRPNAH
                     SSLP"
BASE COUNT         1718 a         1864 c         1704 g         1633 t
ORIGIN      
        1 aagaacttca agctaagctg ctaagagaaa ttcaagaact ctacgaaaaa cgcgggaaga
       61 tcgaagaaga catcagggca ttggaagcac aaagcaaact gcgcatcgaa aaggcagcac
      121 ttcaggaaat tgacgcctat aagaacctga atcgtacgcg cgcgcagcca tcctaagctt
      181 agaagcaatc ccggtggagc caaagtttgt ccgctgcagc gactgttcgg gtcacatcgc
      241 ttacgcccac aacgctcacc gcctcatgtc ctgcaacacc tgttcttacc actccacagg
      301 atcattttct ccatagtcac tgattagttc agcgagccat gacctccagt ttaaaagctg
      361 tacgatgcaa ttcggcgaca gcgtcgatga acagagtagt gagccggtgc aggaaagctc
      421 gcttggagta gttgccgatt tcgcaagctc cgcgcggttc ttccgctatg ctcagccgat
      481 tctatccaat ccgtgcatgt acggctgaag aacctgcggg attgcgacgc tgccatcctt
      541 ttgctggtaa ttttccaaaa cggccacgac agttcggcct acggcaagtc cggagccgtt
      601 tagagtatgg acaagcgtca gcttcccctt atccttgcga aaacggattt gtgcacggcg
      661 tgcttgaaaa tcttcgcagt tcgagcacga actaatctca cgataagtgc cttgtcccgg
      721 caaccaaacc tcgatgtcgt aagtttttgc ggctgcaaat cccatgtcac ccgtacaaag
      781 ttccacgacg cggtaagcca attcaagccg ttttaaaact tcttcagcat tctctaccat
      841 ggcctccaac tcatcatagg agaattccgg ctcgcacagc ttgaccattt cgactttgtt
      901 gaactggtgt tgccgaatca gcccgcgcac atctttgccg taagaacccg cttcacggcg
      961 aaaacacgga gtacaggctg tatactttat cggcaaagcg tcgcgttcca aaatctcctc
     1021 gcggtgaaca ttagtgagag gcacctcggc cgtcgggatc aagaaataat ccggagtctc
     1081 aatccgaaac aggtcctctt caaacttggg caaattgccg gttcccgtca ccgcagcgcg
     1141 gttcgcgagt gcaggcggaa ttatttcctt gtagccgtgt tcgacggtgt gcaaatccaa
     1201 catgaagttg atcaatgcgc gctcaaggcg agcagccgca cccttataga taacgaaccg
     1261 tgcaccggct agtttggcgg cgcgttggaa gtctaaaatg tctaactctt caccgatttc
     1321 ccagtgattt cttgcaggaa agtcaaactc cggcggtgcg ccccaacggc gaacttccct
     1381 attgtcgtct gaatccttgc cgactttaac atcggagttg ggaagattcg gcacggttaa
     1441 cataaagttt tcgaatcgcc cttcaacttc cgctagcggt gtttcgagat cacgaacttg
     1501 cttgctaagc tcctcaactt cccgcatcag cccggtggca tcggcgccgg ttttctttat
     1561 aatgccgatc tcgcccgagc gtcgattttt ctgctctttg agcttttcta atctactcaa
     1621 cacatcacgg cgttctcgat cgagcgaaac gaattcttcc caatcgatca ctataccgcg
     1681 ggtcgccatc cgccttttca cttgctccag atcatcccgc aacagcctta tgtccagcat
     1741 caaataatcc tagattagct cgaatgcgtt ctcaatgtgt tcgacccgcg gttgggctcc
     1801 gggccagcaa ataccgacga catcgaatct ggcctcccgc tgattgagtt ttttcgcggt
     1861 caaaaagaac tgagccgcat aaatcattct ctgctgtttc ctaacttcga cagcttccag
     1921 tggcgttcca tacccgacgc ccttacgcgt cttcacttca acaaaaacga tcacccgccg
     1981 gtccaacata atgagatcca gctctcccgc agcgcaacga tagttgcgct caactagttt
     2041 gtagcctttg cttttcaaga agagctcggc aatccgctca ccctctttac ctaaaacgat
     2101 tttggctctg tttaggaaca cgggttgacg aacggcaaag cgagatccgt ttcgagctct
     2161 aatgcgcaca tcgcacggac cggcctgaag cttcggcgat gaatcggcga cggtccaaat
     2221 cgccgcaaag cttccatatg cgtgatgcag ctatatccct tgtgatgggc aaagccgtac
     2281 tccggataac tcttgtcgag ttcgagcatc atggtgtcac gcgaaacttt ggcgagaatc
     2341 gaggccgcgg ctatcgagac acaaagctga tcgcccttga taatagttct ctgaacagga
     2401 ggtttcttga agagcgtgtt tactgaaaaa aactcggcgg gaatcatctg attgccatcg
     2461 atgagcaaac aatcgggcac cggccgcagg gcccgcaagg ccttggccat cgcgagcaaa
     2521 ctagcctcga gaatattaat tcgatcgatc tcctcgacct cgacaacacc caccgcccag
     2581 catacggcgg actctctaat cgtcggcagc aatcgctcgc gctgctcggt cgttaacatt
     2641 ttcgaatctt tgatgtcggc gtgagtgaaa cccatcggca ggacgacggc cgccgcgacg
     2701 acgggcccgg ctaaaggccc ccggccgacc tcatctactc cggcaacgca gctgaagcca
     2761 cgcgagtatg actgtcgttc aatggctccc tgcattaacg gtcgatcgaa gtcttcagac
     2821 aatgtatatt gtttcccgcc catgtcttcc cttttagcca aaagagaaaa caactgcaaa
     2881 taagaaagtt tactcgacag cgattgattt catcgcaaga ctaactattc aaaaacgggg
     2941 gctgccagct attgatttct cgtcaacagc gattcgaatt ggatttgagt ttgcccatgt
     3001 gttagaaaat ttagatgaga cttaatagcc aagatgtcgc acgggttgcg cagttggctc
     3061 gattgcgcct gcttcccgaa gagttaaatc agctcaccat cgagcttcaa tctattctgc
     3121 agtacatgga taaactcagg gaaattgata cttcggaaat tgagcccttc actcaggccg
     3181 tagatagctc caatgcattt cgtgacgact tggttaccaa caatcctaac acggacgcaa
     3241 tattggcgaa cgcgccagcg acggcgaaga cgttttttaa agttcctaag atcatcgaat
     3301 gagtctgttc tcactgacaa tccatgaagc cagcgaaaaa ctgcgtaagc gcgaagtttc
     3361 ctcgcaagag ctgacggaag ccattttcca gcgcctcgct gaaaccgacg gtctgatccg
     3421 cggctacatc accctttgcc gcgattcggc gatcgcacag gctaaagaag ccgatgaacg
     3481 gctgaagcgg gaagcatcgg cttctccttt gctcggaata ccagtggctc ttaaagataa
     3541 tctcctgacc caagggttaa ggacaacttg cgcatcaaag atattgcaga attttattcc
     3601 tccttatgac gccacggtcg ttacaaaagt ccgctcggcc ggcgcagtga ttacaggcaa
     3661 gaccaacctg gatgagttcg cgatgggttc atctgctgaa aactcggctt ttttcccgac
     3721 gcgcaacccc tggcacttgg aacgcgtacc cggcggatcc tcaggtggat ccgcagcctc
     3781 agtggccgct gatcaatgca tcgcagcatt aggcaccgac accggcggtt caatccgtca
     3841 accagcggct ttctgtggca tagttggcct gaagccaacc tacggcaggg ttagccgcta
     3901 tggcgtcatc gcctttgctt cttccatgga tcaagtaggt cccatgacta aggacgttcg
     3961 cgactgtgcg ctgctgctcg aagccattag cgggcacgac ccagcggatt caacttccgt
     4021 cgattttccc gtaccgtcgt actccaaatc gctaacgcgc gatattacag gtttacgcct
     4081 gggcattcct aaggaatatt tcagttccgg gttgcagccg gccattgagc aagcccttgt
     4141 gatcgccatt cgtcagcttg aaagcgatgg cgccattatc gaggaaatct cattgccgca
     4201 taccgattat gctgtcgctg tgtactacat catcgcaact gctgaggcta gctcaaacct
     4261 cgcgcgttac gatggaatgc gctatggcca gcgcgcccca ggcggcgatt tgacggaaac
     4321 ctatacgctt agtcgggcaa aagggctcgg gccagaggta aagcgacgca tcatgcttgg
     4381 aacctacgcg ctttcagctg gatattatga tgagtactat atgaaggcac aaaaggttcg
     4441 caccttggtt cgccgggatt ttgagacggc attcaaacat tgtgacgcaa ttatcactcc
     4501 tacctcgccg agtaccgcat ttaagatggg cgagaagaca caaaatcccc tgcaaatgta
     4561 cttgtcggat atttgcacga tttcgatcaa tctcgccggc gtgccggcga tttcggtacc
     4621 ctgcggtctc gacggtgaag gactgccgat cgggatgcaa gttattggaa aacactttga
     4681 cgagccaacc atcttgcggg tcgcgtatgc ttacgaacaa caaacggatt ggcacaggaa
     4741 aaaaccggct ttttctgacg catgaactac gaaaccatca tcggccttga agtccatgct
     4801 caacttttga gcgactcgaa gattttttgc ggctgctcca ccaggtttgg tgcggcgccg
     4861 aaccacaata cttgcccagt ctgcgccggc tttcccggcg ccttaccggt gttgaacaag
     4921 aaggtcgtcg agtttgcctt gcgtgccggg ctggcgatgc actgtaagat cgccggatcc
     4981 agccggttgg caaggaagaa ttacttttat cctgatttac ccaaaggtta tcagattagc
     5041 cagtacgagc tcccgatctg cagcaacggc tacatcgatg tcgatgttgg gggcgcaacc
     5101 aaacgagttc gtttaatccg aatccatatc gaggaggatg cggggaagaa cattcacgat
     5161 gcctacgaag attcgagctt gatcgatcta aaccgtgccg gagtaccact gctggagatc
     5221 gtcagtgaac ccgacatccg caatgccggc gaagcagtaa gctatttaaa aaggttacgc
     5281 gcaattcttc gatatctgga agtctgtgat ggtaacatgg aagaaggcag cttccgctgt
     5341 gacgcaaaca tctcgttacg caggattgga acggggtccc tgggaaccaa agtggaaatc
     5401 aaaaacctga actccttcaa ggccatcgaa aaagcactgg agtacgagac ggaacgtcag
     5461 acagatacgc tttcaacagg tggtgacctg gttcaagaaa cgcggctctg ggacgacaat
     5521 aacgagcaaa cccgctcgat gcgatctaag gagtcggctc acgattatcg atacttcccg
     5581 gaccccgatc tcttaccgat caatctggac caagcgtgga cgcaagaggt atgtagcaat
     5641 ttgcctgagc ttccagatgc aagaaaagta cgcttcatgt cgcagtatgg actttcggcc
     5701 tatgacgccg agctgcttac tggccgcaga gatgttgccg actatttaga ggccgcgatt
     5761 aacatctgcc cgaatgccaa agcagtgagc aattggataa tgggcgatct ttttcgcgtc
     5821 ctaaaagaac gaaagcttga tgaacagctc cgtatagaca attggccgat ccagccgaca
     5881 caactgggcg atttggtaca actcattgat gacgggaaga tcagtggaaa aattgcgaaa
     5941 ctcatctttg aggccttgct cgacacgaac caatctccgc aagtcatcat gacccaaaga
     6001 ggcttggagc aggtctcaga tcccgcaagc cttgagaggt ccattgatca ggtcttgata
     6061 acccatgcgc aacaggtagc tgcctaccgc ggtggtaatg acaagatttt tggctttctt
     6121 gtgggtcagg ttatgaaagc ctcacagggg aaagctaacc cgcaaaaggt caatgaatta
     6181 ttgaaatcaa aactttcttg aagtgtggac ttgaacagat cctttttgaa gctacacaac
     6241 acctgcgaac tacggtagag aagaatgcgc attaggtcga taaggctggg gaagatttag
     6301 aatgactccg accagcccac cctctcggat acttttaagt tgccgctcgg catcatctcg
     6361 caatcggaaa tttccaatcc gtactcgcaa tgaatcggct gttccagctt cagtgatgga
     6421 cacggcatag ccgcgctgtt cgaaagtgtc tgcccaacgg cgtgcttccg agtggtcctg
     6481 gaaagacatt tgaataatgt agccttcaag ggctttgggc acgggtctta cgagcgattt
     6541 catttccggt acgtcttcaa tccgtttagc atgacccagc acaggttgct gttcaaaagt
     6601 atctagctcg gcagcgttct gctgcttcga gggtttgatc agcacggggg ccgtcgcctt
     6661 ggtatctgcg gatctcagat ccatcttcgg aacggatgct acttcgtccg gcctctcact
     6721 ttcgctgcca ggctttacct gctgagtagg ggataactgc gtttcagtcg atcccggtac
     6781 aggcggcgta tggaatccca catcaacttg cgaaaactcg gtcggccgca attctgaaat
     6841 cgaagcccga agctgagaat cgattgaatt tgaagccgat tcgagttggt ctcttttttc
     6901 agctagttca accaacttg
//
LOCUS       DASUVS010000170         2950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_503814, whole genome shotgun sequence.
ACCESSION   DASUVS010000170 DASUVS010000000
VERSION     DASUVS010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503814"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VF182_09430"
     CDS             complement(<1..555)
                     /locus_tag="VF182_09430"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437338.1"
                     /translation="MPREIAFLQGLRDLGWVERQNLTIERRYWENRAERLPALADELV
                     RLNLDLIVTTSGTAAWAVKKVTNTIPIVMITSADAVTQGLVASLARPDGNVTGLTNIS
                     TDVIGKRLEILKETFPRVSRVAVLYCPASGKSIAETRWKEIEAAAQALRLYLQPIEVS
                     GPPEIDRALRTATSERADAVFVHDC"
     gene            complement(758..1750)
                     /locus_tag="VF182_09435"
     CDS             complement(758..1750)
                     /locus_tag="VF182_09435"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437339.1"
                     /translation="MKRKIFALTLCAMLFALCGFASAQQTGKVFRIGFLDNSTAAGSA
                     VLLEAFRQELNKLGWIEGKNITIEYRFAEGKFDRLPDLAADLVRLKVDLIVVSGGQPA
                     LAARSATTTMPIVMANAGDPVAFGLVASLGRPGGNVTGLSSLVPELNSKRLEILSDTV
                     PKLIRVGFLGLSGGGGPAGEATMKEIRPAALGLKIKLEEIQTQLDAKGLESAFQTAKQ
                     KQVGAIMTTTGNPFFTERKRIVELAVKHRLPAIYPQKEFVDAGGLMSYGANYDDLYRR
                     AAVYVDRILKGAKPADLPVQQATKFEFVINLKAAKQIGLTIPNNLLERANKVIK"
     gene            complement(1782..2357)
                     /locus_tag="VF182_09440"
     CDS             complement(1782..2357)
                     /locus_tag="VF182_09440"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437340.1"
                     /translation="MTGLSRLTPELSGKQLELLKEIIPKLSRVAVFGSSSQPGNGQAL
                     TEIQLAAKTLGVEVQYSDVLSPKDIETGFRTARNGRADAVMWLVAGLIGTSHRKEVVD
                     LTVKSGFPAIYNQPGYVEAGGLMTYGATLSDLDRRAATYVDKILRGAKPADLPVEQPK
                     KFELVINLKAAKQIGLTIPPNVLARAARVIR"
     gene            complement(2375..2845)
                     /locus_tag="VF182_09445"
     CDS             complement(2375..2845)
                     /locus_tag="VF182_09445"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX6437341.1"
                     /translation="MNAFPTKSLSGNHTFKSCPKHGRSIQNRKWVGFSLIAFMLAVVA
                     AVAQAQQLTNIPRVGWLSGSSRSAISVRSDAFRQGLGEVGYLEGKNIVVEWRYGEGKP
                     DRVSEFAAELVHLKVDVIVTAGPTPTRFAKKATKTIPIVMHRTAIRSETVLLSA"
BASE COUNT          649 a          868 c          697 g          736 t
ORIGIN      
        1 gcagtcgtgt acgaacactg catcagcgcg ttcgctggtt gctgttcgaa gcgcacgatc
       61 aatttccggg ggaccgctca cctctatcgg ctgaaggtaa agtcttagag cttgcgctgc
      121 ggcttctatt tccttccatc gcgtttccgc tatcgatttt cctgacgccg gacagtacaa
      181 gacggctacg cgtgagactc tcgggaaagt ttccttgagg atttcgagtc gcttcccaat
      241 cacatccgta gagatgttgg tcaaccccgt gacattaccg tccggacgcg cgaggctcgc
      301 gacaagtccc tgagtgaccg catcggcgga ggtaatcata acaataggga ttgtatttgt
      361 gactttcttg acagcccatg ccgctgttcc gctcgtcgtc acaatcaagt ccaagttcag
      421 acgaaccaat tcatcagcaa gagccgggag tcgctcggcc cggttctccc aatacctgcg
      481 ttcgattgta aggttctgcc tttcaaccca gccgagatcg cgaagtccct gaaggaaggc
      541 tatctctcgg ggcatgctgg acggagatcg atcgattccg agaaagccga tttgagcgcc
      601 ttccggatcc ctgctgcgcc tcgctaataa cactaatcgc taggagcaga atgactagac
      661 caacttggat catcggtttt gtcgctctca cttcattatc ctgcccgccg tggccagcac
      721 gcacggcgga atctctgttt ctgaacggct gtctactcta tttgatcact ttattcgctc
      781 gttcgagcag gttgtttgga atcgtcaaac caatctgttt tgccgccttt aaattaatga
      841 cgaactcaaa cttcgtcgct tgttgcaccg gcaaatcagc cggtttggca ccttttaaga
      901 tcctgtcgac gtaaaccgct gcgcgtcgat agaggtcatc gtagttcgcc ccataggaca
      961 tgaggccgcc cgcatcaaca aactctttct gtgggtaaat agcaggcagt ctgtgtttga
     1021 cagcaagctc gacaatccgc tttctttcgg taaaaaaggg attaccagtg gtggtcatta
     1081 tcgcgcccac ctgcttctgc tttgcggttt gaaaagcgct ctctaaacct ttggcgtcga
     1141 gttgagtctg gatctcctcc aattttatct tcaagcctag agctgcaggt ctgatctctt
     1201 tcattgtggc ttctcctgcc gggccgcccc ctcccgacag gcccaaaaat ccaactcgga
     1261 tgagcttggg gactgtgtcg gaaagtatct ctagcctttt gctatttaac tcgggcacta
     1321 aactcgacag cccggtgaca ttgcctccgg gccgcccgag actggcaacc agaccgaagg
     1381 ccacggggtc tccagcattc gccatcacaa tgggcatagt cgtagtggcg ctcctggccg
     1441 ccaatgccgg ttgcccgccc gaaaccacaa ttagatcaac cttcagccgc accaagtccg
     1501 cggcaagatc aggaagccga tcaaattttc cttcggcaaa tcgatactcg atggtgatat
     1561 tttttccctc aatccaacca agcttgttca gctcttgccg aaacgcttcc aacaggacgg
     1621 cactcccagc agcagtgcta ttatccagga aacctatgcg gaagactttc cctgtctgct
     1681 gcgccgatgc gaaaccgcaa agcgcaaaga gcatcgcgca aagcgtcaat gcaaagattt
     1741 ttctcttcac tcttttctac tcacgccttt cgcctcacgg ctcaccgaat taccctggcc
     1801 gctctcgcca gcacgttcgg cggaatcgtc aggccgatct gcttggctgc tttcagattg
     1861 atgaccaact caaacttctt cggctgctca acaggtagat cggctggctt agcgcctctc
     1921 aaaatcttat cgacataagt cgcggcgcgc cggtccaagt cgctcaaggt cgcgccgtag
     1981 gtcataagcc cgccggcttc cacatatcct ggctggttat atatcgccgg aaatccgctt
     2041 tttaccgtga ggtctacaac ttcttttcga tgagaagtgc cgatcaagcc cgccactaac
     2101 cacatgacgg cgtcagctcg cccattccgt gcagtgcgga aacctgtctc gatatctttg
     2161 gggcttagca catcggaata ttgaacctcc acgccgagtg tctttgcggc gagctgaatt
     2221 tctgtcaagg cttgcccatt gcccggctgg ctcgaacttc cgaagacagc cacacgcgaa
     2281 agcttgggga tgatctcctt caaaagctcc agttgttttc cgcttagctc cggtgtcagc
     2341 ctcgataatc cagtaatgtt cccgcccgga cgcgctaagc tgacaacaaa accgtttccg
     2401 acctgatcgc cgtcctgtgc atgacgatcg ggatcgtctt tgtcgctttt ttagcaaaac
     2461 gagttggtgt tggaccagcg gtgacgatga cgtccacctt cagatgcacc agttccgctg
     2521 caaactcgct tacgcgatct ggttttccct ctccatatcg ccactcaacg acaatattct
     2581 tcccctctag atagccaact tcgcccagac cctgccggaa tgcgtcactg cgtaccgaga
     2641 tagcagaacg agaactacca gatagccatc ctacccgcgg gatgttcgta agctgctgtg
     2701 cctgggctac ggcagccacc accgccagca taaaagcgat taatgaaaac cccacccatt
     2761 ttcgattttg gatacttcga ccgtgcttag gacagctttt aaacgtgtga ttgccggaaa
     2821 gagatttcgt aggaaatgcg ttcatcggtt tgtctcccgg ctttcaatcc aaatcccaaa
     2881 tcgaaaatcc aaaattgttt catcgaatta ttctatccgc ccgcgccagc acgtttgtgg
     2941 ggatcgtcaa
//
LOCUS       DASUVS010000171         3747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_503850, whole genome shotgun sequence.
ACCESSION   DASUVS010000171 DASUVS010000000
VERSION     DASUVS010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503850"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VF182_09450"
     CDS             <1..811
                     /locus_tag="VF182_09450"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437342.1"
                     /translation="GKNIAFELRYAEEKPQRLSVLADELVRLKLDVLVAGGPNDGIAA
                     KKATKTIPIVFTDTPADPVPRGLVDSLARPGGNITGFYSMADVLAGKRVEALKEALPK
                     LSRVAVLWYPSAESGQPQWAESQRAARQLGLQIHSMEIGSMENYEGAFKEAIKAGSAA
                     LAVTRHRLPGTNAVRKQIFELTAKYRLPAIYYSEDFVEQGGLMSYGADEVEPFKRVAW
                     MIDKILKGAKPADLPVEQPTRFALVINLKTAKQIGLTIPPNVLARADRVMR"
     gene            808..1839
                     /locus_tag="VF182_09455"
     CDS             808..1839
                     /locus_tag="VF182_09455"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437343.1"
                     /translation="MKRLRFFNFGFCTPGRAKSLFFIYVLCVWLLGFSTPLDAQQSGK
                     MSRVGFLANTRTTNSIEPFRRGLQALGYTEGKNIHIDYRYLETKRELIPSLVSELVQT
                     KVDVLVAGIPSAIRAAREATKTIPIVIVTNQDPVAVGYVESLARPGGNITGITNMTRE
                     LGGKRLELFRDVVPKLSRVGILWNARSAETGFGRGIQDYEAKAKALNIPLESLPIRAA
                     NLDFEGAFRSAAKARVSAVITLAHIFLSPYRREIAGLAIKHQLPSMGEIEEYAEAGGL
                     ISYAAEDDESYRRAAWYVDKILKGAKPGELPIEQPTKFELVINLKTAKQIGLIIPPNI
                     LARADRVIR"
     gene            2017..2994
                     /locus_tag="VF182_09460"
     CDS             2017..2994
                     /locus_tag="VF182_09460"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437344.1"
                     /translation="MKNILSTFLIIAVVVVGTIAEAQQPGKIFRIGFLDSTTASGMGG
                     TLEAFRQELSKLGWTEGKNITIEYRFAEQKFERTPELAAELVRLKVDLILVSGSQPAL
                     AAKSATNTIPIVMTNAGDPVAAGLVASLARPGGNITGNSGLEAQLNTKRLEVLKDAVP
                     RLAQVGLLWSPRAAELQLKELRSAALALKLKLEEIETQVDAQSLESAFQTAKRKKVGA
                     IITTSGRTFFAERKRIVELAGKYSLPAIYYQKEFVAEGGLMFYGTDFDDMFRKSAHYV
                     DKILKGAKPADLPVQQATKFEFVINLKAAKQIGLTIPNKLLERANKVIK"
     gene            3061..>3747
                     /locus_tag="VF182_09465"
     CDS             3061..>3747
                     /locus_tag="VF182_09465"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437345.1"
                     /translation="MKLTKPMIQVGLILVLIAISVTGEAQHGPLAQIGFLGTDRSPST
                     MPREKAFLQALRDLGWIEGQNLTIERRYWQNRADRLPALADELVRLNLDAIVTSSGTA
                     ARAVKKATNTMPIVMITSADAVTQGLIASLARPGGNVTGLTNVSTDVIGKQLEILKEA
                     FPRVSRVAVLHCPASGISITEIRWKEIEAAAQALRLYLQPIEVSGPPEIDRALRTATS
                     ERADAVFVHDC"
BASE COUNT          942 a          967 c         1048 g          790 t
ORIGIN      
        1 aggcaagaac atagctttcg agctgcggta tgcggaagag aaacctcagc ggctttctgt
       61 gttggcggat gagctggttc gtctcaaact cgatgtcctc gttgcgggtg gtccaaacga
      121 tggcatcgcc gccaagaagg ctacaaaaac gatccccatt gttttcaccg atacccccgc
      181 cgatccggtt ccgcgcggct tggttgacag cctggcgcgg cctggaggaa acatcacggg
      241 cttttattcg atggccgacg ttctagctgg caaacgagtg gaggcactca aggaggcttt
      301 acctaaactc tcccgtgttg ctgtattgtg gtacccgagc gccgaaagtg gccagccgca
      361 gtgggcagaa agccagcgcg ccgcacgaca acttgggctg caaattcact ctatggaaat
      421 cggcagcatg gagaactatg agggcgcctt caaagaggca ataaaagcgg gtagcgctgc
      481 tcttgcagtg acgcggcatc gtttgcccgg aaccaacgca gtccggaaac agatcttcga
      541 gctgacagca aagtatcggt tgccggcaat ttactatagt gaggatttcg tcgagcaagg
      601 tggcttgatg tcttacggcg cggacgaagt cgagccgttc aagcgcgtgg catggatgat
      661 cgataaaatt ctcaaaggtg ccaagcccgc cgatcttccc gtcgagcagc cgacaagatt
      721 cgcgctagtg attaatctga aaaccgccaa gcaaatcggt cttacaattc ctcccaacgt
      781 gttggcaaga gcggatcgag taatgcgatg aaacggctcc ggtttttcaa ttttggattt
      841 tgcaccccag gtcgcgctaa aagtcttttc tttatttacg ttctttgtgt gtggctgttg
      901 gggttctcta ctcctctcga tgcacaacaa tcaggcaaga tgtctcgggt cggatttctc
      961 gcgaacacca ggacgacaaa cagtatcgag ccgtttcgcc gtgggctgca agcactcggc
     1021 tataccgaag gaaaaaacat ccatattgac tatcgctatt tagaaaccaa acgggagctg
     1081 attccgtctc tggtatccga actggtgcaa acgaaggtcg atgtacttgt tgctggcatc
     1141 ccgtccgcaa tccgcgcagc ccgggaagct accaaaacaa tcccgatcgt catagtgacc
     1201 aatcaggacc ccgtcgcggt aggctacgtc gaaagtcttg cgcgacccgg tggaaacatc
     1261 acgggaatca ccaatatgac ccgcgagttg ggaggcaagc ggctcgaact gttcagagac
     1321 gttgtgccga aactatcgcg cgtcggcatt ctatggaacg cgcgatcggc ggaaactgga
     1381 ttcggccggg gaatccaaga ttatgaagct aaagctaagg ctttgaacat tccactggag
     1441 tcgttgccta tccgcgcggc aaatctcgac ttcgagggag cctttcgcag tgccgcgaaa
     1501 gcgagagtca gcgccgtcat cacgctcgcg cacatctttc tcagcccata tcggagggaa
     1561 attgccgggc tagccataaa gcaccagctg ccgtcgatgg gcgagataga agaatatgca
     1621 gaggcaggcg gactgatatc gtatgcagcc gaggatgatg aaagttaccg ccgggctgca
     1681 tggtacgtcg ataagatcct caagggcgcc aagccaggcg agcttccgat tgagcagcca
     1741 accaagtttg agctagtgat caacctcaaa accgccaagc aaatcggatt aataattcct
     1801 ccgaatattt tggccagagc cgaccgagta attcgatgac cttagaatct aaaatcccaa
     1861 aatctaaaat ccaaaatgcg aatgatcccg ccgagcgtgc tcgcgcgggc ggacgcggtg
     1921 attaaatgac cgtgctgaat aacgagtgtt gaggactgag ttggagatgg ggaaagactc
     1981 aggactcatc gctcagcact tcggcggaga gtcgcgatga aaaatattct ttccactttt
     2041 ctcatcatcg cggtggtggt tgtgggcacg atcgccgagg cgcagcagcc cggaaaaatc
     2101 tttcgcatcg ggtttctgga ttcaaccact gcttccggta tgggagggac tttggaggcg
     2161 ttccgacaag agttgagcaa gcttggatgg actgaaggaa agaacatcac catcgagtat
     2221 cggtttgccg agcaaaagtt tgagcgcacc cccgagcttg cggcggaact ggttcgtctt
     2281 aaagttgatt tgatcctggt ctcagggtca cagccggcgt tagcggccaa gagcgccacc
     2341 aataccatac ccattgtgat gacaaacgcc ggggaccccg tggctgcagg attggtcgcc
     2401 agtttggcac ggccgggagg caatatcacc gggaactcgg gtttagaagc ccaactaaac
     2461 acaaaaaggc tggaagtact caaggacgca gttcccaggc tcgcccaagt tggacttctg
     2521 tggtcgccgc gagcagcaga attgcaactc aaagagctta ggtcggcggc tctggcactg
     2581 aagctgaaat tggaggagat cgaaacgcaa gtagacgccc aaagcttgga gagcgccttt
     2641 caaactgcaa agcgaaaaaa ggtgggcgca attataacaa cctccggtcg caccttcttc
     2701 gctgaacgca agcggatcgt cgagcttgcc gggaagtaca gcttgccagc tatttactat
     2761 cagaaggagt ttgtcgctga gggcggtctc atgttctatg ggacagactt cgatgacatg
     2821 ttccgcaagt ctgcccacta cgtggacaag atattgaaag gcgccaagcc ggcggatctg
     2881 ccggtgcagc aggcaacgaa gtttgaattc gttatcaact taaaggcagc gaaacaaatc
     2941 ggtctgacaa ttccgaacaa gctcctcgag cgcgcaaata aagtaatcaa ataaatcttc
     3001 tacagcgatt cagaaatcag agatcagggc cgtacgtggg acggggccag ggatgatcaa
     3061 ttgaagctga caaaaccgat gatccaggtt ggtctgatcc ttgtgctcat agccatcagt
     3121 gttactggcg aggcgcagca tggaccgctc gctcaaatcg gctttcttgg aactgatcgt
     3181 tccccgtcca ccatgccccg ggagaaagcc ttccttcagg cacttcgtga cctcggttgg
     3241 atcgaagggc aaaatcttac aatcgagcgc agatattggc agaaccgagc ggaccgactc
     3301 ccggcccttg ctgatgaact ggttcggctg aacttggacg ccatcgtgac gagtagtgga
     3361 acagccgcgc gcgcggtaaa aaaggctacc aatacgatgc cgattgtgat gattacttcc
     3421 gccgatgccg tcactcaggg acttatcgcg agtctcgcgc gtccgggcgg caatgtcaca
     3481 ggcttgacga acgtgtctac cgatgtcatc gggaaacaac ttgagatcct taaggaagct
     3541 ttcccgagag tctcacgtgt cgccgtcttg cactgtccgg cgtcagggat atcgataacc
     3601 gagattcgat ggaaggaaat agaagccgca gcgcaagctc taagacttta ccttcagccg
     3661 atagaggtga gcggtccccc ggaaattgat cgtgcgcttc gaacagcaac cagcgaacgc
     3721 gctgatgcag tgttcgtaca cgactgc
//
LOCUS       DASUVS010000172        21413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1596499, whole genome shotgun sequence.
ACCESSION   DASUVS010000172 DASUVS010000000
VERSION     DASUVS010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 21413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21413
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596499"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VF182_09470"
     CDS             complement(<1..938)
                     /locus_tag="VF182_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437346.1"
                     /translation="MATYVRKSAYRRDPITAMLLFVVMLLFGSACREAFAGSAAEPTR
                     LRISIPGLGSSSYPLIMAQKKGYFRAEGYEVELIPMAAGLAVKTLLAGEVGVTAAGTV
                     LANLQGAKVRLVMGFIRELPYDLVVPPEIRRVEDLRGKKIAVADRGSVTFFVVRSILQ
                     AHGMEPDRDVTLLNMGTPAVRHQALLAGIVHGVVANFDGTALLVDRGYHSIAKAGSFM
                     KGFAGSLSVTQEQLEKRPDDVFRAVRATLKGIRAYLAQRDEAIKYSSAWTKISAANAA
                     KVHELTSAGALAPDGLLDRETMESVIHESREVAGIKR"
     gene            1079..2215
                     /locus_tag="VF182_09475"
     CDS             1079..2215
                     /locus_tag="VF182_09475"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6437347.1"
                     /translation="MKNGFKIIDSDMHIMEPLDLWEKYIDPRFKERAPRPVRVPTRGD
                     LNLLMVDGKEPRQCSPTLAHDVRMISGRRNNTRGDVIDFARKQNFNAESQLEAMDIEG
                     IDVAVLFPTACLHIMTMPEMDPLLAEAICRAYNDWLHDFCQENPKRLFGAALLPPHDV
                     SLAVREARRAREKLGFVTAFLRATPLPGQTWFSVYWEPLWAELEEIGMTVGFHECTNS
                     GHYPHLERFGTGNRLLRHICTHVTGQMVTMVDIIVGGVLERFPQLKVAFLECNCGWAP
                     SWLQRMDRHWQQLGKHDAPLLTIKPSEYFKRQCVIGCEGDEADIGNVCQLIGDDNLVF
                     SSDYPHSDSDFPKATQEFFHQEMPADSRRKILWDNCVKFYGIEG"
     gene            complement(2279..3235)
                     /locus_tag="VF182_09480"
     CDS             complement(2279..3235)
                     /locus_tag="VF182_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437348.1"
                     /translation="MCRAGFCATLTLGILLFACQTSIAQTRLRASYGSLAVSQVVLPL
                     GARAGIFQKNGLHLEPIYIGGRSVSALISGDVQFGFMGGPPAILAHVGGADVVMLAGL
                     NGLDQILVALPIIKRGADLVGKRVGISRFGTTADYGARIGLKKLKLQAQKDVTLIQIG
                     DTPARIGGMLSGAIEAATLNSSEKELAFRNGFHILADPSDVEFPGNAIVTTRSFMKTN
                     REDVKRFVRALVEVIHFAKTQPERGKKLLQEIYRQNDTGVIAKRYDAMVEMFPDYPYL
                     TRGSVLSFLEILRDEGKLKDPLYPDPFLDTSLLSEVERERKK"
     gene            complement(3256..4368)
                     /locus_tag="VF182_09485"
     CDS             complement(3256..4368)
                     /locus_tag="VF182_09485"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6437349.1"
                     /translation="MSKNGFRAFDSDMHVFEPADLYTKYMNPKWGERIPRGQPRTKHG
                     MTRYFLGNGKPIRPPSDVVDFHEKRMAERFNESLARDYDNISQLAAMDKEGLDIAVLF
                     RTSPLHTNEKFEPEYANDLCKAWNDWMADFCKADSRRLKGSALITLHDVDLAVNEAKR
                     AVENGAVGLSLCPEPINGRQIHDRYFDPLWQEAQEMNIPICFHPPARPEQPQVSKDRF
                     EGHPNQALLVNPLRNPIEQILAVSAFTAGGVLERFPKLKVAFLEGNCSWLPWLLYRID
                     EYWEMQGAMANIPLRKKPSEYFKEQCFISVDVDEALVKNVIDYLGDDNIVISTDYPHI
                     DCRWPHALDEFLTIPISEQSKKKILWDNCARLYNHH"
     gene            complement(4808..5740)
                     /locus_tag="VF182_09490"
     CDS             complement(4808..5740)
                     /locus_tag="VF182_09490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6437350.1"
                     /translation="MANLHTPCGIELPQVFFDGPTDMEHIRSFAMRAETLGYDSLWLQ
                     ERIIGDFTMLEPVTLLSYVAGITTKLKLGTSVILLPLRNPLQLAKAYSSLDVMSGGRA
                     ILGVGLGGGHLGSHEDVFGYSREGRVTRFTEAVQIMKLLWMEPTASFQGRYWSFKEIS
                     MEPKPLQKPHLPIIFGGHHDNALGRAVKTANGWMGAGSSSSASFIREAARIRIFLDQA
                     QRDPASFHFAKRVYLAVDNDKARGEKRIREWFARRYKNADLGPKVSIWGSSAECAEKI
                     QEIVHAGAQHIVLNPMFDEMEHLEICAKEIMPHL"
     gene            6071..6508
                     /locus_tag="VF182_09495"
     CDS             6071..6508
                     /locus_tag="VF182_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437351.1"
                     /translation="MKRAEKSGSPVKKPARRRNIDVQTEQGQQSTMATASSTLKATPR
                     STDRPEESSIFKERKRLFLGVGDEVTHLRYEHWGIGVVMECMTSSVPGGTCLARIRFQ
                     DGTLRCFNNDLDNDSCCYYFGLRRYWNPTHGVNAIRSRLFMRG"
     gene            6512..6844
                     /locus_tag="VF182_09500"
     CDS             6512..6844
                     /locus_tag="VF182_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437352.1"
                     /translation="MLKTAKTARRLIVAALFVGSLSPSILNAEEAIEIAAVDVGVTAG
                     NLVFLPAKAASVAMGLVSGALSFVLTGGNADLTRQIWRDTTEGPYLITPQQARKAIGE
                     RPELMEPK"
     gene            complement(6910..7644)
                     /locus_tag="VF182_09505"
     CDS             complement(6910..7644)
                     /locus_tag="VF182_09505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6437353.1"
                     /translation="MAQTVDNIVDPNDSSLTSEMVQFPGSAGNVSAYVSRPKDGKNLG
                     TVIVIHENRGLVGHIKDVARHFAKEGFAAIAVDCMGRIGGSDQYNGSDEATEAIKKVN
                     GGMVAEDLTAALNYMKKQSYVNGKVGVVGYCWGGGQSLNFATKNKDLNAAVVYYGRNP
                     DPLDSVANIPCPVMGNYAEDDANIMPGVEPLKAAMQKAGKSFDVKTYPGAKHAFNNNT
                     NADRYNPEAAKDAWGRTVSFFKKNLA"
     gene            complement(7941..8273)
                     /locus_tag="VF182_09510"
     CDS             complement(7941..8273)
                     /locus_tag="VF182_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6437354.1"
                     /translation="MPVYEYECQKCGTFETTQRITEEPLSKCPTCKGKVKKLISNTSF
                     QLKGTGWYITDYARKGKDAPKTENGASSSSSESKESKKESKETKPETKKESAPSKSES
                     SPTSTSTS"
     gene            complement(8343..8738)
                     /locus_tag="VF182_09515"
     CDS             complement(8343..8738)
                     /locus_tag="VF182_09515"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6437355.1"
                     /translation="MDFVNLGEKQYEARETKKRKIDLFGTDNFRSWMLYFVPGDGTDM
                     HYHASPETFLVLEGSGTVKSIKGDERLVKKHDVLFLGAKDYYQITNTGTEPLVLFGNR
                     SEAFGGAHILADGKDRTKSNNEPATSSGS"
     gene            8924..9631
                     /locus_tag="VF182_09520"
     CDS             8924..9631
                     /locus_tag="VF182_09520"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6437356.1"
                     /translation="MTSSWESTTVDNNTMPLYLSVPERGGPVPGIVVVHGQSGLESFI
                     KETTHMLALQGYAAIAPDLYHRDGPDCKDDNATKKSRVRDAGIIQDINGAMQFLKNRR
                     QVDGARLGIVGFCMGGRIVYLMSAATRDLKAAVMFYGSSTMTPFGEGPSPFDRTREIS
                     CPIQGHFGADDQNPSPEDMRKLDGELTRWNKPHEFHTYAGAAHAFANAGSANYRPHAA
                     ALAWPKAMEFFSRHLIG"
     gene            9888..11465
                     /locus_tag="VF182_09525"
     CDS             9888..11465
                     /locus_tag="VF182_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX6437357.1"
                     /translation="MAENSEGSGSARHHMHYYNAAADLIERNLAVRPEKIAYIDENGR
                     YTFAELAERVNRCAHVLCELGATLESRVMVCLSDTIDFPSAFLGAIKAGLVPVAVNTL
                     LTTGDYDFMLRDSRARILVVSESLLPVFKPTLAGQPFLSHVVVSGPSASDEPRLAELM
                     AAANNEFTVAPTCPDDACFWLYSSGSTGTPKGTVHVQTSMVATTELYAKPILGIEESD
                     VVFSAAKLFFAYGLGNGLSFPLSVGATTVLLGERPTPAAVCWILRQHRPTIFYGVPTL
                     YGALLASAELPKRDELNLRRCTSAGEALPPNVGRKWLEHTGVDILDGLGSTEMLHIFL
                     SNRPGDVRYGTSGKPVPGYEVRLLDEDGNKVAKGEIGELQVSGPSSAAYYWNNREKSR
                     TTFLGRWTRSGDKYIESEDGYFTYCGRADDMLKVSGIWVSPFEVEAVLVSHEAVLEAA
                     VVGRADDNGLIKPKAFVVLRGDLTQTAELTAELQQHVKTHLAPHNYPRWIEFVAELPK
                     TATGKIQRFKLREPMGK"
     gene            11506..12690
                     /locus_tag="VF182_09530"
     CDS             11506..12690
                     /locus_tag="VF182_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437358.1"
                     /translation="MDLNSSRRQFLVRAGLLTASALLPNPPALAQTQKIRIGLMLPYT
                     GTYAQLGTAITNGFKLTVAERGGKLAGRELEYFSVDDESEPAKAPENANRLVQRDKVD
                     VLVGTVHSGVQMGMVKVAKESNTLMIIPNAGLDAATGALCAPNIFRTSFSNWQPSFPM
                     GKVLADRGMSNIVTLAWKYGAGQEAVAGFNEGFIKAGGKSVRGLWIPFPNVEFQALLT
                     EIASIKPDAVFVFFAGAGAAKFIKDYNAAGLRRQIPLFGSGFLTDGVLDAVDKAAEGL
                     ETTLHYADGLNTKRDREFRAAYDKAYKSEPDVYAVQGYDAGLLLNIGLDAVKGDVTKK
                     KEMIAAMEKAKIDSPRGPWTMSKAHNPIQDIYLRRVVGRENKMVSIAHKALADPARGC
                     KM"
     gene            12875..13732
                     /locus_tag="VF182_09535"
     CDS             12875..13732
                     /locus_tag="VF182_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6437359.1"
                     /translation="MFYLIQLLNGIQYGLLLFLLASGLTLIFGIMGVINLAHGSFYMI
                     GAYLAYWLARTTGSLWLAIPLSIIIAFSIGLLLETTIFSRLYKRDHLYQVLLTFGLIL
                     VFEELRSILFGDDVHGVPVPPLLDFSIPLTETLSYPVYRLFVTAVCLAIALTMYFVIH
                     KTRLGMMIRAATSNREMAASLGVNIPLLFAFVFAFGVALAAFAGTISAPIASVSPGMG
                     NHIVIICFVVVIIGGIGSINGAMIAALAIGLADTLGKVTAPEYSAVAVYLLMAAILLW
                     RPQGIAGRA"
     gene            13746..14744
                     /locus_tag="VF182_09540"
     CDS             13746..14744
                     /locus_tag="VF182_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6437360.1"
                     /translation="MTLPRSVLLVAAAAIAVFPLVGSTFYVQLGAKIMIMAIFALSLD
                     FLVGHTGLVSLGHAAFFGLAAYVLALISPQYQAANLWLTLPVAVVITALAALVIGFLV
                     VRTTGVYFIMVTLAFAQMLYAIFHDTNLGGGSDGIYIYVRPEPAVAGTRPFNLESPEQ
                     FYYFVLLIMVGVYALLSKVLHAPFGRALAGIKANEHRMRSIGYPVSSYKLGAFVFGGA
                     IAGVAGYLAACQFGFVNPEILAWHHSAGVLMMVILGGMGKLHGAVLGAFAYILLQEIL
                     STPLLLGSYAKHWQLAMGSLIVFIVLALPHGLAGILDSSLRSRSSKPARVEEAADD"
     gene            14737..15495
                     /locus_tag="VF182_09545"
     CDS             14737..15495
                     /locus_tag="VF182_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6437361.1"
                     /translation="MIESILQVRQLAKKFGGLAAVDEVSLDVNVGQLHAVIGPNGAGK
                     TTLINMLSGDLSPSAGSIRFGGTEVAHLSPERRSRLGIGRSYQKVNIFAAFSALENCR
                     LAAQSRDPHPWNWLRQASSYDQIMANARSALADVGLTNRADTLAFTLSHGEQRQLEIA
                     MSLATQPKLLLLDEPLAGLGIEESSSMVDLIRRMAEMHAILLVEHDMDAVFQLARVLT
                     VMVDGRVLASGKPGDVRGDEQVQRAYLGKFDEHR"
     gene            15492..16208
                     /locus_tag="VF182_09550"
     CDS             15492..16208
                     /locus_tag="VF182_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6437362.1"
                     /translation="MTLRSIIEAQGLHTYYGASHILHGIDFLVRRGEAVGLMGRNGMG
                     KTTLIRTLVGHIAPRHGAIKINDRVMTRAAPYLIARQGIAYVPEGRGIFPNLSVRENL
                     IMMARPGPNGQCDWTYERVLQTFPRLAQRLSYGGQQLSGGEQQMLCIGRALMTNPTLL
                     VLDEATEGLAPLIAQEIWRIIRTVRESGIAAIIVDKNFGAVSALTDRNIILVKGRIVF
                     EGTGSELRKRPELLQQHLGI"
     gene            16309..16977
                     /locus_tag="VF182_09555"
     CDS             16309..16977
                     /locus_tag="VF182_09555"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6437363.1"
                     /translation="MDNQKFVDSIIQEIVQPGVDQLINNRFFTELRQGKLSQKRLKGF
                     ALQHYHCNHAINKGLAFCMVRNANNPPLYNQFQELFTEEQSHPGLMKRFGLAIGLTDE
                     DFDRELLIFECVAHTAAVIRGMFLGTQAESRAGALVNETMVCRYSGEFDTYLERHYGF
                     DSRARTFFIVHSKVDQEHTAMASESIARNANTDRDKEMVRAAARNMVRFKLAKFEGIY
                     DAYA"
     gene            17201..18193
                     /locus_tag="VF182_09560"
     CDS             17201..18193
                     /locus_tag="VF182_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437364.1"
                     /translation="MIKMIPTALRLLALSTLLISATELAWCAEKTSKLAPVRIAYVSR
                     SILDMPYVIARDRGFFREEGLEAEFVFMKAIQTVQTMLAGGVDFGTATGTAISAAVNG
                     ADVRVVFALTDKPSFDMIAQPNVTNVQQMRGKKLGISAPGSLTEILARQILLVNKIPL
                     DQVTMLSLGTSDVTYVALKAGTIDATMLQVPQNLISQEEGYRKIASGADVYRAVQGGL
                     TTTKTVLSERPEVVTRVIRATQKALRLIRNDKKFTVEFIKGPFLDLGKDRDKYADRVY
                     DTALAFYLQSGVVDEKLQRDMIATAAQRVKPKDPVPPERVFDFSFAQKVADSMK"
     gene            18272..19426
                     /locus_tag="VF182_09565"
     CDS             18272..19426
                     /locus_tag="VF182_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX6437365.1"
                     /translation="MKFSTDRILTTHAGALPQPDDLKQMHTAKISGQPIDEAAFAQRV
                     RQSVAEVVRKQLACGLDIINDGEVGKSNFSRYARERLSGFIERDAKPEDHVSSIFARD
                     MVEFGDYFRRRMGGIRGENVKRVFCNGPLKYVGHASLQAEIEDFKAGLQGQSYVEAFL
                     PAIAPGTIEHWMKNDYYPDDESYLAAIADAMHEEYKAIVNAGFLLQIDDPDLPDGWQF
                     MSRMSVPEYRRYAELRVDALNHGLRDIPPEKVRFHTCWGSYHGPHKFDIPLRDIVDII
                     LKVNANTYSIEASNPRHEHEWRVWQDVKFPQGKVIVPGVVGHATDIVEHPEVIAERLL
                     RYARIVGRENVMAGTDCGLGPRVGDPQIAWAKFEAMAEGARIASKELWGR"
     gene            19537..19977
                     /locus_tag="VF182_09570"
     CDS             19537..19977
                     /locus_tag="VF182_09570"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX6437366.1"
                     /translation="MTTDRFSAIALGVFALLVIWDSLQLPLGTLRQPGPAFIPILLAS
                     LLLIFAILLVLTSSRAPSLSSVKWTEWRHALAILAASLFSVFAIERLGYRLTVLLVLG
                     FLVKLVEQRGWIVSLSFAFTLSFASFFLFYTVLRVPLPQGPFGF"
     gene            20256..>21413
                     /locus_tag="VF182_09575"
     CDS             20256..>21413
                     /locus_tag="VF182_09575"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6437367.1"
                     /translation="MTETLHNLYVGFSVALAPNVLLYAFVGCVIGTLVGVLPGVGPLA
                     GISLLLPVSFGLDATSAIVLLAGIYYGAMYGGSTTSILMRIPGEAASVMTCIDGYEMT
                     RKGRAGAALAIAAVGSFIAGTVSIVGLMLLAPPLASFALRFGPPEYFTLLLLGLLALA
                     YMSGGSMLKSLAMASLGLLIGMIGIDPMTGFIRFSYGLIELGDGIGVVPVAVGLFGIS
                     EILLTAGQPSTPRVKQPRLKELLPSRAEWRDSFWPFGRGTVLGFLIGIIPGSAHIIAS
                     FVSYAMERRLSKHPERFGHGAVEGVAGPESANNAATSGAFVPMLALGVPSGPIPAVMI
                     AAMMVHGISPGPLLIAQQPELFWGFVASMYVGNLVLLILNLPLVGIFVTILR"
BASE COUNT         4645 a         6009 c         5776 g         4983 t
ORIGIN      
        1 cgtttaattc cggcgacttc gcgggattcg tgtatcactg attccatggt ctcacgatcg
       61 agcagcccat ctggcgccaa tgcgccggcc gacgtcagct cgtgaacctt cgcggcattg
      121 gccgctgaga tcttggtcca cgccgaactg tacttgatcg cctcgtcgcg ctgcgccagg
      181 taggcacgga tacctttcag cgtagcgcga accgctcgaa acacgtcgtc gggccgcttc
      241 tccaactgtt cttgcgtcac cgagagcgac ccagcgaagc ctttcatgaa gctcccggcc
      301 ttcgcgatgg aatggtaacc gcggtcgacc agcagagcgg tgccgtcaaa gttcgccact
      361 acgccgtgaa caatcccggc cagaagcgct tgatgacgca ctgccggcgt tcccatgttg
      421 agtaacgtga catcccgatc cggctccatg ccatgcgctt gcaaaatcga ccgcacgacg
      481 aagaaagtca cgctgccgcg gtcggccacc gcaatttttt tgccgcgcag atcttcgacc
      541 cgtctgatct ccggcggcac cacgagatcg tacggaagtt cgcgaataaa gcccatcacc
      601 aagcgcacct tcgcgccttg caagttcgcc aacacagtgc ccgcggcagt gacaccgact
      661 tcgccggcca gtaaggtttt taccgcaagc ccggctgcca tgggtatcag ctcgacttca
      721 tagccttcag cgcggaaata gcctttcttc tgcgccatta tcaaagggta agatgatgag
      781 ccgaggccgg gaatgctgat gcgcaagcgc gtgggctccg cggcactccc ggcaaaagcc
      841 tctcggcatg cgctaccgaa tagcagcatc acgacaaaga gcaacatcgc tgttattggg
      901 tcacgcctat atgctgattt acggacgtat gttgccatcg tgccgactcc ctagtgactc
      961 gctgcgcgaa tctgtattcc tcctggttcg ctcatgtcaa gcttacggtt gacagcgcct
     1021 ccgcgctagt tgtagttgga gataaccgaa cggcgtcatt ttgacgtggg gaagaccaat
     1081 gaagaatgga ttcaagatca tcgactccga catgcatatc atggaaccgc tggatctttg
     1141 ggaaaaatac atcgacccgc gcttcaaaga gcgcgcgcca aggcctgtgc gtgtgcctac
     1201 gcgcggcgat ttaaatctgc tcatggtcga cggtaaagaa ccgcgccaat gtagtccgac
     1261 cttggcccat gacgtgcgta tgatcagtgg caggcgaaat aacacccgcg gcgatgtcat
     1321 cgacttcgcg cgcaagcaaa atttcaacgc cgagtcgcag ctcgaagcga tggatatcga
     1381 aggcatcgac gtggccgtgc tcttccccac cgcgtgtctg cacatcatga ccatgccgga
     1441 aatggacccg ttgctcgccg aagcgatctg ccgcgcttac aacgattggc tccacgactt
     1501 ctgccaggaa aatcccaagc gtcttttcgg cgccgcgctg cttccccctc acgatgtctc
     1561 gcttgccgtg cgcgaagcgc gccgcgctag agagaagctc ggattcgtaa ccgcgttcct
     1621 gcgcgccacc ccgttgccgg gacagacttg gttcagcgtc tattgggaac ctttatgggc
     1681 ggaactagaa gagataggca tgaccgtcgg ctttcacgaa tgcaccaatt caggacacta
     1741 cccgcatctt gaacggttcg gcacaggcaa ccgcttgctg cgtcacatct gtacgcacgt
     1801 caccgggcaa atggtgacaa tggtcgatat aatcgtcggc ggcgtattgg aacgttttcc
     1861 gcagctgaaa gtcgcattcc tcgaatgcaa ctgcggctgg gcgccttcat ggctgcagcg
     1921 catggaccgg cattggcaac agctcggcaa acatgacgcg ccgctgctga cgataaagcc
     1981 cagtgaatac ttcaaacgcc agtgcgtgat cggctgcgaa ggggacgaag cagatatcgg
     2041 taacgtctgc caattgatcg gcgacgataa tctggttttt tccagcgact atccgcacag
     2101 cgactcggac ttccccaaag ccacccagga gttttttcat caagagatgc cggcggacag
     2161 ccgcaggaag atattgtggg ataactgcgt caagttctat gggatcgagg gttaacgcga
     2221 ttcaaagttc aagctgaagc gccgatcata ctcttgattc cttgaactcc ggaaccagct
     2281 atttttttcg ctcccgctct acttccgaca acaagcttgt atcgagaaat gggtccgggt
     2341 aaagcggatc tttcagtttc ccttcgtcgc gcagtatttc caggaatgac agcaccgaac
     2401 ccctggtcaa atagggatag tcgggaaaca tctccaccat ggcatcgtaa cgcttcgcaa
     2461 tcactccggt atcattctgg cggtagatct cttggagcag ttttttgccg cgctcaggct
     2521 gagttttcgc aaaatgaatc acttcgacca aagcacgcac gaatcttttc acgtcctccc
     2581 tgttggtttt cataaatgac ctcgtggtca cgattgcgtt gccgggaaat tcgacatcgc
     2641 tcggatcagc caggatgtga aagccattcc tgaaggccag ctccttttca ctgctgttca
     2701 acgtcgccgc ctcgatggcc cccgacaaca tccctccgat gcgcgccgga gtgtcgccga
     2761 tctggatcaa cgtcacatct ttttgcgcct gcaacttcag cttcttgagc ccgatgcgag
     2821 cgccataatc cgcggtagtg ccgaaacggc tgatgccgac ccgtttaccc accagatcgg
     2881 caccgcgttt gatgatcggc agagcgacga gaatctgatc gagaccgttc aacccggcaa
     2941 gcatgactac atcggcacca ccgacgtgcg cgaggatcgc tggcggacca cccatgaagc
     3001 cgaattgcac atcgccgctg atcagggcgg agacggatcg gccaccgata tagataggtt
     3061 ctaaatggag gccatttttc tgaaagatgc cagcgcgggc gcccaagggc aaaaccactt
     3121 gcgatacggc tagggaaccg taagacgcgc gcaggcgggt ttgggcgatg ctggtctgac
     3181 atgcgaacag caaaatgccc aaagttagag ttgcacagaa tccggcacgg cacatagcga
     3241 gctcctccgc gatgattaat gatgattgta cagccgcgcg caattgtccc agagaatttt
     3301 tttctttgat tgctcgctga tcggtatcgt cagaaactca tcgagcgcgt gcggccagcg
     3361 gcagtcaatg tgcgggtaat cggtggaaat gacgatatta tcgtcgccca aataatcgat
     3421 cacgttcttg accagcgctt catccacgtc cacagagata aaacactgct ccttgaaata
     3481 ctcactgggc ttcttgcgta aaggaatatt tgccatcgcg ccctgcatct cccaatactc
     3541 atcaatccga taaagcaacc acggcaacca gctgcaattg ccttcgagaa aagccacttt
     3601 cagtttaggg aaccgttcca acacgccgcc cgcagtgaag gcgctgaccg ccagaatttg
     3661 ctcgatcgga ttgcgcagcg gattgaccaa aagcgcctga ttaggatggc cttcgaagcg
     3721 atctttcgac acctgcggct gctccgggcg cgccggcgga tgaaaacaga tcggaatgtt
     3781 catttcctgc gcttcctgcc acagcggatc gaagtaacga tcatggatct gccggccgtt
     3841 gatcggctcc gggcaaagcg acaaaccgac tgcgccgttc tctacggcac gtttagcttc
     3901 gttcacggca agatcgacgt catggagcgt aatcaacgct gatcctttga gacgcctgga
     3961 atccgctttg cagaaatcgg ccatccaatc gttccatgcc ttgcacagat cgttggcgta
     4021 ctccggctca aatttctcgt tggtgtggag cggtgaagtg cgaaacagga ctgcgatgtc
     4081 caaaccttcc ttatccatgg ccgctagttg agaaatattg tcgtagtcgc gcgccagcga
     4141 ttcgttgaag cgttcggcca tgcgcttttc atgaaaatcc accacgtcgc tcggcggccg
     4201 aatcggcttg ccgttcccta agaaatagcg cgtcatgccg tgctttgtgc gcggctggcc
     4261 gcgcggaatg cgctcgcccc atttgggatt catgtacttc gtgtacaaat cagccggctc
     4321 gaagacatgc atatcgctgt cgaaggcgcg aaagccgttt tttgacatgc caaattcctc
     4381 ccttcatgcg agtcgagtat cagataggtc gcttctatcg catcgcatct tgtgtaagca
     4441 aggatcaaaa ctcacctaaa aggaatcgcg cgcagcacag gaggcaatca atttgcttga
     4501 cctcttcgaa agtcaaagat ttagaagcct gctctttgcg agagcgaagt gcctagacac
     4561 ccgactcaga aagcgcacca tccgtttgcg ggggtcgcca ttatcaactt acatgtggca
     4621 acctaacgtg ggcgtcctga caagtcttta gcgaagaagt gaccgtcaac ttttgtcgat
     4681 tttcaaaaac agctggagaa tagagacccg cgtacgttct agtgagactc ctctttagtt
     4741 ggcgtcttta cgcctttacg cgagaaattc cgacctcgtt gcgatcattt ctctgagtta
     4801 gattcactta caaatgtggc atgatctctt tggcgcagat ttccaaatgc tccatctcat
     4861 caaacatggg attgaggaca atgtgctgcg cgccggcgtg aacgatctct tgaatctttt
     4921 cggcgcattc ggcgctgcta ccccagatcg agaccttggg tcccaaatca gcgtttttgt
     4981 aacgtcgagc aaaccattct cgaatacgct tctcgccgcg agccttatcg ttgtcgactg
     5041 cgagatagac tctcttggca aaatgaaagc tagccggatc gcgctgcgcc tggtctaaga
     5101 aaatacgaat acgcgccgcc tcgcgaatga aagatgcgga agaagacgac cctgcgccca
     5161 tccaaccatt ggcggtcttc actgcccgcc caagggcgtt gtcgtgatgg ccgccaaaga
     5221 tgatcggcaa gtgcggcttt tgcagcggtt tgggttccat ggagatttct ttgaagctcc
     5281 agtaccggcc ttgaaaactc gccgtcggct ccatccaaag aagcttcatg atctgcactg
     5341 cttcggtaaa gcgtgtcact cgcccttccc ggctgtagcc aaaaacatcc tcatgagatc
     5401 cgagatgacc accgccaagc ccgacgccca aaattgcgcg gccgccgctc attacgtcca
     5461 agctcgaata cgccttggct aactgcaacg ggttacgcag cggcaacaga atgaccgacg
     5521 tgcccaactt caatttggtt gtgatcccgg cgacatagct cagcagtgtc accggttcga
     5581 gcatggtgaa gtcgccgatg atccgttcct gcagccataa gctgtcgtaa ccaagcgtct
     5641 ccgctcgcat cgcgaaactg cgaatgtgct ccatgtcggt ggggccatcg aaaaaaactt
     5701 gaggaagctc gatgccgcaa ggtgtgtgca ggttggccat atgttgggtc cttcctcttg
     5761 aagtttcctt aatcgctcag aacggaaact cgcccacttg cgcgcgcttc gctaacgagt
     5821 tctgaagttc acactttcat gttccaatac tcagtcgctg accaacattc agcagtaaaa
     5881 ggttcagcgc gcccggacct ctcgccttca tactgatcgc gcctctcggt gtcaactcag
     5941 tttcggcccg aaactttcgc ccgctcagac atgtcagagt tttttatttg gagcttgact
     6001 gtcacccggc attttggtga atatgagggg gtgaatggag caaggcagca gatggttcac
     6061 aggagttttt gtgaagcgcg cggaaaaaag cggatcgcct gtaaaaaagc cggctcgaag
     6121 gcgtaacatc gacgtccaga cggaacaagg gcagcaatcg acgatggcta ctgcgtcttc
     6181 aacattaaaa gccactcccc gcagcactga tcgccccgag gagagttcaa ttttcaagga
     6241 aagaaaacgg ctttttttgg gcgttggcga cgaagtcacg catcttcgtt atgagcactg
     6301 gggcatcggt gtggtgatgg aatgtatgac atcgtcagta ccaggcggca cctgcctcgc
     6361 ccggattcgc ttccaagacg gcacactgcg ctgttttaac aacgatctcg ataacgatag
     6421 ctgctgttac tatttcggcc tgcgccgtta ctggaacccg acgcacggag tgaacgcaat
     6481 ccgctcaagg ctttttatgc gaggttagtg catgttgaaa accgccaaaa ccgctcgccg
     6541 cctaatcgtt gccgcattgt ttgtcggcag tctgtctcca tcaattctga acgcggaaga
     6601 agccatcgaa atagccgctg ttgatgtcgg cgtaaccgct ggcaatctcg tgtttcttcc
     6661 ggctaaagcg gcctccgtag cgatgggctt ggttagcggc gctttatcgt tcgtgctaac
     6721 cggcggtaat gctgatctga cgaggcagat ttggcgcgac accactgaag gtccttatct
     6781 catcaccccg caacaagcgc gtaaagccat tggcgaacgt cccgaactga tggaaccaaa
     6841 ataaaaacgg catgtaaccg ccatcgttga gatggagcca catgccgttt tcatcgtacg
     6901 tccgatcgat taagccaagt tcttcttgaa aaaactaact gttcttcccc aggcgtcctt
     6961 agccgcctcg gggttataac gatcagcgtt ggtattatta ttgaacgcat gtttcgcccc
     7021 ggggtaagtc ttaacatcga acgactttcc cgccttttgc atcgccgctt tcaatggttc
     7081 aacgcccggc atgatgttcg cgtcatcttc agcgtaattg cccatcaccg gacagggaat
     7141 atttgccact gaatcgagcg gatcgggatt gcgtccgtaa taaaccacgg cggcgttgag
     7201 atctttattt ttggtggcaa aatttaatga ttgaccgcca ccccaacagt aaccgacaac
     7261 gcctaccttg ccgttcacgt agctctgctt cttcatgtaa ttaagagccg cggtcagatc
     7321 ctccgcgacc atcccaccgt ttactttctt gatcgcttcg gtggcctcgt cgctgccatt
     7381 gtattgatcg ctgccgccga tgcgccccat acagtccacc gcaatcgctg caaagccttc
     7441 cttggcgaag tgtcgagcga cgtctttgat gtgaccaacc aggcctctat tctcatggat
     7501 gacgataacc gttcccaggt tcttgccatc cttgggtcgt gaaacatagg cactgacgtt
     7561 ccccgcactg ccaggaaact gtaccatctc cgacgtgaga ctcgaatcgt tcggatcaac
     7621 gatattgtct acagtttgag ccatcttagt tctctccttt cgcgtgttat ggatggccca
     7681 gctatagcca aaatcaaccc acaaatccag cgaaggatga cttgtttgga agaaataatt
     7741 gagcgccaaa tgccgccagt caagcaccga catcgcagac ctgttgcctt cgttccggag
     7801 ggttcgtccc ttgatcgctg tgtcaccctg aactgcggag ttcgggttca acagattaaa
     7861 acaattcaca gaagctaaaa cgccgcgctc ataattagcg cggcgttggt gagacttact
     7921 cgatcggagc tataatctac tcaggaggta ctcgtggatg tgggcgaaga ttcggattta
     7981 gatggagcag actctttctt ggtctcgggt ttcgtttcct tgctttcctt tttggattct
     8041 ttggactctg aggacgacga acttgcgccg ttctcggtct tgggagcatc tttacccttg
     8101 cgcgcgtaat cggtaatgta ccagccggtg cccttaagct gaaacgatgt gttcgagatc
     8161 agcttcttta ccttcccttt gcaggtcgga cacttggata atggttcttc ggtaattctc
     8221 tgagtcgttt caaaagttcc acacttctgg cactcgtatt catagacagg catgctagtt
     8281 cctcaattac gatggtcaat ccataagcta atcacagaca cccggcttgt caatcgatac
     8341 ggttagctcc cggaactagt tgctggttcg ttgttcgatt tggttctatc cttgccgtcg
     8401 gcaaggatgt gggcgccacc aaacgcttca gagcggttgc caaagagcac cagaggctcg
     8461 gttccagtat tggtaatctg gtaataatcc tttgcgccaa gaaacagcac gtcgtgtttt
     8521 ttgactagtc gctcatcgcc tttgatgctc ttgaccgtgc ccgatccttc gagcacgaga
     8581 aaagtctccg ggctggcatg atagtgcatg tccgttccgt caccgggaac gaagtagagc
     8641 atccatgagc gaaaattatc ggttccgaaa aggtcgatct ttctcttctt ggtttcgcgc
     8701 gcttcgtatt gtttttcgcc caagttgaca aagtccatga ttaatctccc tgttggaaag
     8761 tattcgattc taacttacag gcgcagtact acgcaaggat tgacaatctg ccctgacagg
     8821 tgtggagtgc tgcactattg agacaagcgt ttacttgatc acaatcaaca acctgataat
     8881 cgcaacatcg ggctttgact gccgacgaac gcggaggtga gccatgacca gctcttggga
     8941 aagtactacc gtcgataaca acacaatgcc gctgtatctc agtgtacccg agcgtggcgg
     9001 tccagtgccc ggcattgtcg tcgttcacgg acagtcgggc ctggaaagtt tcatcaaaga
     9061 aacgactcat atgctcgcgc tgcagggtta tgccgccatc gcgccggacc tgtaccatcg
     9121 cgacggtccg gattgcaaag acgataacgc aaccaaaaaa tcgcgagtgc gcgacgccgg
     9181 tattatccag gacataaacg gcgcgatgca gtttttgaaa aaccgccgac aggtagacgg
     9241 cgcgcgtttg ggtatcgtcg gtttctgcat gggcggacga atcgtttatc taatgtcggc
     9301 cgcaactcgg gacttgaaag ccgctgtgat gttttacggc agcagcacga tgacgccgtt
     9361 cggcgagggc ccatcgccgt ttgatcgcac acgcgaaatc agttgcccta ttcaaggaca
     9421 cttcggcgcg gatgatcaga acccatcacc cgaggatatg cgcaagctcg atggcgaact
     9481 cactcgctgg aataagccgc acgagtttca cacttatgcc ggcgccgctc acgcctttgc
     9541 taatgcaggt tccgccaatt accggcctca cgcagcggct ttggcatggc ccaaagccat
     9601 ggaatttttc agccggcatt tgatcggtta gagaatcatt caagcgcgcc gaagaaagag
     9661 gtgcacgatc gtaaagatcg tgcgccgttg gatttgcatg ctgccggagc agttcattgc
     9721 gcatttttca aggtcgttgc gcgatgcgcg ccacacccgg aacatcctca acacctgcca
     9781 gcagccgtct tgatgctcgc ggtccttcca tagtaagaaa tgattctagc catggctggc
     9841 aagctatgag cgttatccga agaatccgaa ttacgaggaa ctgacggatg gctgagaatt
     9901 cagaggggtc gggatcggct cgtcatcaca tgcattacta caatgccgca gccgacttga
     9961 ttgaacgcaa cctcgctgtg cgccctgaaa aaattgccta catcgacgag aacggccgtt
    10021 acacctttgc cgagctggca gagcgcgtca atcgctgcgc ccatgtcctt tgtgagctgg
    10081 gtgcgacctt ggagtcgcgc gtcatggttt gtttgagcga taccatcgat ttcccgagcg
    10141 cgtttcttgg cgccatcaag gcagggctcg ttccggtcgc ggtaaacacg ctgctcacca
    10201 ctggtgatta cgacttcatg ctgcgcgaca gccgcgctcg catactggtg gtatccgagt
    10261 cattacttcc ggtattcaaa ccgacactgg cgggacagcc gttcctaagc catgttgtgg
    10321 tctccggccc gagcgcttcc gacgaacctc ggcttgcaga gttgatggcg gccgctaaca
    10381 acgagtttac cgtcgcgccg acctgtcccg atgacgcctg cttttggctt tactcttcgg
    10441 gctccacagg tacaccgaaa ggcactgtgc acgttcagac cagtatggtt gcaaccacag
    10501 agctttacgc caaaccaatc ttgggaatcg aggagagcga tgttgtattc tcggcggcga
    10561 aattgttttt cgcctatggt ctgggtaacg ggttgagttt tccgctttca gtgggcgcga
    10621 ccacggtctt gctgggcgag cggccgacgc ctgctgcggt ttgctggatt ctgcgccagc
    10681 atcggccgac aatcttttat ggcgtgccga ctctttatgg cgcgctcctg gccagcgcgg
    10741 aattgcccaa acgagatgag cttaacctgc gacgttgcac gtcggccggc gaagccttgc
    10801 cgccaaacgt cggccgtaag tggctcgaac ataccggagt agacattctc gatggcttag
    10861 gttccacgga aatgcttcat atctttttat ccaatagacc cggcgacgtg cgctacggga
    10921 cttccggcaa accggttccg gggtatgagg tgcgactgct cgatgaggac ggcaacaagg
    10981 tggccaaagg tgagatcggc gaattgcagg tcagcggccc cagcagcgcc gcatactact
    11041 ggaacaatcg cgagaaaagt cgcaccacgt ttctcggcag atggaccagg agcggcgaca
    11101 aatacatcga aagcgaagac ggttatttta cctattgcgg gcgcgccgac gacatgctca
    11161 aggtcagcgg catctgggtc tccccattcg aggtcgaagc agtgctggtc tcgcacgaag
    11221 cggtgctgga agccgcggtc gttggccgtg ccgatgataa cgggctgatc aaacccaagg
    11281 cattcgttgt tctacgcggc gatttaacac agacagccga gctcactgcc gaactacagc
    11341 aacacgtcaa aacgcatttg gcgccgcata actatccccg ctggatcgag ttcgtggccg
    11401 agctgccaaa aaccgccacc ggcaaaatcc aaaggttcaa actgcgagag ccaatgggga
    11461 aatgacccgc gctgaacttt tcgggctacg gagacaatca tgaacatcga tctcaactca
    11521 agccgccggc aatttcttgt cagggccggc ctacttacgg caagcgcatt gctgccgaat
    11581 ccgccggcac tcgcacagac tcagaagatt cgcatcggcc tcatgctccc ttacaccggc
    11641 acttatgccc aactgggcac cgcaatcacc aacggcttta aactgactgt ggcggagcgt
    11701 ggcggtaagc tcgccggtcg cgaactcgaa tacttttccg tcgatgacga atccgaaccg
    11761 gctaaagcgc cggagaacgc caataggctg gtgcagcgcg acaaggtgga cgtgctggtc
    11821 ggcacggttc acagcggcgt gcagatgggc atggtcaaag ttgcgaaaga aagcaatacc
    11881 ttgatgatca ttcccaatgc cggcctcgat gccgctaccg gagcgctgtg cgcgccgaat
    11941 atctttcgca cttcattttc caattggcag ccgtcctttc caatgggcaa agtgctcgcg
    12001 gaccgcggca tgagcaatat tgtgacgctg gcttggaaat acggcgctgg tcaggaagcc
    12061 gtcgccggat ttaatgaagg ctttatcaaa gccggcggca aatcggttcg tgggctttgg
    12121 attccgtttc ccaatgttga atttcaagca ttgctcaccg aaatcgcttc gatcaaacca
    12181 gacgcggtgt tcgtgttttt tgccggcgcc ggcgcggcaa agttcatcaa agattataat
    12241 gcggctgggc tgcgcaggca gatcccgctc ttcggttcgg gttttctcac cgacggcgtg
    12301 ctcgatgccg tggataaagc cgcggaaggc ctggaaacta cgctacacta tgccgatggc
    12361 ctgaacacca agcgggatcg cgagttccgc gcggcctatg acaaggcata caagagcgag
    12421 cccgacgtct acgcggtgca aggctatgac gctggtcttc tcctcaacat tggactagat
    12481 gcagtgaagg gcgacgtcac caaaaagaaa gaaatgatcg cggctatgga aaaagccaag
    12541 atcgacagcc cgcgcggacc ctggacgatg tcgaaagcgc acaatccaat tcaagacatt
    12601 tacttgcgca gagtagtggg gcgcgaaaac aaaatggtca gcatcgcgca taaagcgctc
    12661 gccgatcctg cgcggggatg taagatgtaa ccctcgcagg ccgaagccgg cctgtgaggg
    12721 gttcaagacg ttcaaatcgt tcaagacgtt caagccgtac cggaatcgca aagcgatata
    12781 caaacttctt ttgcataaac cacagacacg aactcgtgcg gcctcttaaa cgttttcaac
    12841 tgcttgaacg atttgaacgg cttgaactgc ctttatgttt tatttgattc aacttctgaa
    12901 cggcatccaa tacggccttc tattgttttt actggccagc ggtctgaccc tgatctttgg
    12961 gattatgggt gtcatcaatc tcgcccacgg cagcttctat atgattggcg cctatctggc
    13021 ttattggctg gcaaggacaa cgggcagcct ctggttggca attccgctca gcatcatcat
    13081 cgccttctca atcggtttgc tgcttgagac aaccatcttc agccggctgt acaaacgcga
    13141 ccatctttat caggtcttgc tcacgttcgg cttgatcctg gtgttcgagg agttgcgcag
    13201 cattttgttc ggcgacgacg tgcatggcgt tccggtgcca ccgctgctgg atttttcgat
    13261 tccactcacc gagacgctct cgtatccggt ctaccgcctg ttcgtcaccg ccgtatgcct
    13321 cgctattgcc cttaccatgt acttcgtcat ccataagacc cgactcggca tgatgatccg
    13381 cgccgccacc agcaatcgcg aaatggccgc ttcgctcggg gtcaacattc cgttgctgtt
    13441 tgcgtttgtc ttcgcattcg gcgtcgcgct cgcagccttt gctggaacga tctcggcgcc
    13501 gattgcctcg gtgtcgcccg gaatgggcaa tcacatcgtg atcatttgtt tcgtggtcgt
    13561 gatcatcggc ggcatcggtt cgatcaacgg cgcaatgatc gcggctttgg cgatcggcct
    13621 ggccgacacc ctcggcaaag taacggcgcc ggaatactcc gcagtcgccg tttatttgct
    13681 gatggcggcg attctcttat ggcggcccca aggaatcgcc ggcagagctt gacgtcgtga
    13741 cccctatcac tctgccgcga tcggtcttac tcgtcgccgc cgcggcgatc gccgtcttcc
    13801 cgcttgtcgg ctcgacattt tacgttcagc tcggcgccaa gatcatgatc atggcgatct
    13861 ttgccttgag cctggatttt ctcgtcggcc ataccggtct ggtgagcctt ggacacgcag
    13921 ctttttttgg cttggcggca tatgtgctgg cgttgatttc acctcaatat caggcagcca
    13981 atctctggtt gaccttgccc gtggcggttg taatcaccgc tctggcggcg ctggtgatcg
    14041 gttttcttgt tgtgcgaacc accggcgtct atttcatcat ggtgacgctt gccttcgcac
    14101 agatgctcta tgctattttt cacgacacca atctgggcgg cggctcggat gggatttaca
    14161 tatacgtgcg gccggaaccg gcagtagccg gcactcgacc tttcaatctg gaaagtccgg
    14221 aacagtttta ctacttcgtg ctgctgatca tggtgggtgt ttatgctttg ttgagtaaag
    14281 tgcttcatgc gccgttcggg cgcgcgctgg cgggcatcaa agccaacgag caccgaatgc
    14341 gctccatcgg ctatccggta tcgagctaca agctcggcgc tttcgtgttc ggcggcgcga
    14401 tcgcgggggt cgccggttat ctcgccgcgt gccagttcgg attcgtcaat cccgagattc
    14461 tcgcctggca ccattcggcc ggcgtgttga tgatggtaat cctgggcggc atgggcaaac
    14521 tccacggcgc ggtgctcggc gcgttcgctt acatcttgct gcaagaaatt ctctcgacgc
    14581 cgttgctgct tggttcgtac gccaaacact ggcagctggc catgggaagc ttgatcgtgt
    14641 tcatcgtgct tgcgttaccg cacgggctgg ccggaattct cgattcctcg ttacgcagcc
    14701 gctcaagcaa gccggcgcgg gtggaagagg cggccgatga ttgagtcgat cctgcaggtg
    14761 cgccaattag ccaaaaaatt cggcggtctt gcagcggtcg acgaggtttc tctggacgtc
    14821 aatgtcggcc aactgcatgc ggtgatcgga cctaatggcg ccggtaaaac cacactgatt
    14881 aacatgctgt cgggcgatct atcgcccagc gccggcagca ttcggttcgg cggcaccgaa
    14941 gtggcgcatc tttcaccgga gcgtcgctcg cgtttaggaa tcggcagaag ttatcagaag
    15001 gttaatattt ttgccgcatt cagcgcgctg gagaactgcc gcttggcagc gcaatcgcgc
    15061 gatccgcatc catggaattg gctgcgccag gcaagctcct atgatcagat catggctaac
    15121 gcccgatccg cgcttgcgga tgtcggttta acaaatcgcg ctgacaccct agcgttcact
    15181 ctcagtcatg gcgagcagcg ccagctggaa atcgctatgt cgctggccac ccagcccaaa
    15241 ctgcttctgc tcgacgagcc gttggcgggt cttggcatcg aggaatcttc aagcatggtc
    15301 gacctgatcc gtcgcatggc agaaatgcat gctattcttt tggtggaaca cgacatggat
    15361 gcggtcttcc aactggctcg cgtgctcacg gtgatggtcg atggcagggt tctcgcttcc
    15421 ggcaagccgg gcgatgttcg gggagacgag caagttcaac gcgcgtacct gggaaagttt
    15481 gacgaacacc gatgacgctc cgctcgatca tcgaagccca aggcttgcac acgtactatg
    15541 gcgccagtca tattcttcac ggcattgact ttctagtgcg ccgcggcgaa gccgttgggc
    15601 ttatggggcg caacggcatg ggcaagacca ctctgatccg cacgctggtt ggtcacatcg
    15661 cgccgcgcca tggcgcgatc aaaatcaacg accgtgtcat gacgcgcgcg gcgccctacc
    15721 tcatcgcgcg ccaaggcatc gcttacgttc ccgaggggcg tggtattttt ccgaaccttt
    15781 ccgtgcgcga aaatttgatt atgatggcgc gaccaggacc gaatgggcaa tgtgattgga
    15841 cctacgagcg ggtgctgcaa accttccctc gtttagccca gcgcttgtcg tatggtggac
    15901 aacaactttc cggcggcgag caacaaatgc tttgcatcgg ccgcgccctg atgaccaatc
    15961 caacgttact ggtgctcgat gaggccactg aaggcctggc gcctctgatt gcccaggaaa
    16021 tttggcgcat catccgcact gttcgcgaaa gtggtatcgc tgccatcatc gttgacaaaa
    16081 acttcggcgc cgtttcggct ctcaccgatc ggaacattat actggtcaaa ggccgcattg
    16141 tttttgaagg caccggcagt gaattacgca agcggccgga actgctgcag cagcatctcg
    16201 gaatttgacc gaaattcccg cttagccgtg cccattgagc cgcttgacac tttatggttg
    16261 ctttgagagg ttcggctgcc ggtgattttc tcctcaagga aggtaattat ggacaaccag
    16321 aagtttgtcg attccattat tcaagaaatt gttcaaccag gcgtcgatca attaatcaat
    16381 aaccgttttt ttaccgagtt aaggcaaggc aagttatcgc agaagcggct caaaggcttc
    16441 gcgctacagc actatcattg caaccacgcg atcaataagg gactggcgtt ctgcatggtg
    16501 agaaacgcca ataacccgcc gctctacaat cagtttcagg agctgtttac cgaagagcag
    16561 tcgcaccccg gcttgatgaa acgtttcggt ttggccattg ggttgactga tgaagatttt
    16621 gacagagagc tgttgatctt tgaatgcgtg gctcacaccg ctgcggtgat ccggggcatg
    16681 tttttaggaa cccaggccga aagccgcgcc ggcgcgctgg tgaatgaaac catggtttgc
    16741 cgctattccg gagaattcga tacctacttg gagaggcact acggtttcga cagccgcgcc
    16801 cgaacattct tcatcgtcca tagcaaagtg gatcaagagc acactgccat ggcttccgaa
    16861 agcatcgcgc gcaatgccaa caccgaccgt gacaaagaga tggtccgtgc cgcagcgcgc
    16921 aatatggtcc gtttcaaact ggccaaattc gaaggcatct atgacgcgta tgcctgagac
    16981 acgcgagcag gctgctgaaa agcctcatat actttcgaaa gaggaatacg aaagctaaaa
    17041 actgaattcc gctttcatcc ctgtgattcg cgcccgttgt ctctggagtc tttttgagca
    17101 gtctgcattt cgaaaatcgc aatgactttt aaaacagact gggtttcgaa gacgctagct
    17161 gaaggggcaa tgacgacgat aacgcctgga ggtggtttgc atgatcaaga tgatcccaac
    17221 ggcgctccgg cttcttgccc tatcgacact tctcattagc gcgactgagc tcgcctggtg
    17281 cgctgagaaa accagcaaac tcgcgccggt tcgcatcgcg tacgtgtcgc gcagcatctt
    17341 ggatatgccc tatgtgatcg ctcgcgatcg aggttttttc cgcgaggaag gtttggaagc
    17401 agagtttgtc tttatgaagg cgattcagac ggtgcagaca atgcttgccg gcggcgtcga
    17461 ctttggcacc gcgacgggta cggcgattag cgccgcggta aacggcgccg acgtacgcgt
    17521 cgtgttcgct ctgacggaca agccgtcctt cgatatgatc gcgcagccga acgtcaccaa
    17581 cgtgcagcag atgcgcggca agaaactcgg catttccgcg cccggctcgc tcacagaaat
    17641 tcttgctcgg cagattctgc tcgtgaacaa gatcccgctg gaccaagtca ccatgctctc
    17701 cctgggcacc agcgatgtta cctacgttgc cctgaaagcg ggcaccatcg acgcgaccat
    17761 gctgcaggtg ccgcagaacc tcatctccca agaagaaggc tatcggaaga tcgcctccgg
    17821 cgccgatgtt taccgagccg ttcaaggagg tctgaccacg accaagacgg tcctcagcga
    17881 acgtccagag gtggtcacga gagtgattcg ggcaacgcag aaggcgctgc gcctgatccg
    17941 caacgataag aaattcacgg tggagtttat caagggaccg tttctcgacc tgggaaagga
    18001 tcgcgacaaa tacgccgatc gagtttatga caccgctttg gcgttctatc tccagtcggg
    18061 agtggtcgat gaaaaattgc agcgcgacat gatcgccacc gcggcgcaac gagtcaaacc
    18121 caaagatccg gtcccgccgg agcgtgtctt cgatttcagc ttcgcacaga aagttgcgga
    18181 ttcgatgaag tagaggatgc agacaagatt acaattctga cggactgaac ccttgaacgt
    18241 tcaacggata gaacaatcat tcggagggat aatgaaattc agcactgacc gaatactgac
    18301 cacccacgcc ggcgcgttgc cgcagcccga tgatctaaag cagatgcata cagcaaagat
    18361 cagcggccag ccaattgatg aggccgcttt tgcgcagcgg gtgcgccaat ccgttgccga
    18421 agtggttaga aaacagttgg cttgcggtct cgatatcatc aacgatgggg aagtcggcaa
    18481 gtcgaacttt tcgcgttacg cgcgggagcg actgagcggt tttatcgagc gtgatgcgaa
    18541 accggaagac catgtctctt cgatcttcgc tcgcgacatg gtcgagtttg gcgactattt
    18601 tcgcaggcgc atgggtggca tccgcggcga aaacgtcaag agggtcttct gcaacgggcc
    18661 gctcaaatac gtcggtcatg cctcgcttca agccgagatc gaagacttca aagccggcct
    18721 gcaagggcag tcttatgtag aggcttttct tccggcgatt gccccgggca ccatcgagca
    18781 ctggatgaag aacgactatt atcccgacga cgaatcgtat ctggccgcca tcgccgacgc
    18841 gatgcacgag gaatataagg cgatcgtcaa tgccggattt ttgctgcaaa tcgacgatcc
    18901 ggatttgccc gacggctggc aattcatgtc gcgaatgtcc gtgccggaat atcgccgcta
    18961 tgccgaactg cgcgtcgacg cgctcaatca cggcttgaga gacattcctc ccgagaaagt
    19021 tcgctttcac acctgctggg gcagttatca tggaccgcac aagttcgaca ttccgttgcg
    19081 tgacatcgtc gacatcatcc tcaaagtcaa cgccaacacc tattcgatcg aggcgtccaa
    19141 tccgcgccac gagcacgaat ggcgcgtatg gcaagacgtc aagtttccgc agggcaaagt
    19201 catcgtgccc ggcgttgttg gacacgcgac agacatcgtc gaacacccgg aagtgatcgc
    19261 cgaacgtttg ctgcgttatg ccaggatcgt tggccgggaa aatgtcatgg ccggcaccga
    19321 ctgcggcctc ggaccacggg tcggcgatcc gcagattgcc tgggcgaaat tcgaagccat
    19381 ggccgaaggc gcgcgcattg ccagcaagga gctgtggggc cggtaaacag agcattggcg
    19441 aagctaaaaa cacgaagatc gaaactgcca tcttgttccg aagatgaaaa gattatctac
    19501 ccagcctgat gcgagcttgc tacaatccgc gccaccatga caaccgatcg gttttcggca
    19561 atcgcgcttg gcgtctttgc ccttctggta atctgggaca gccttcagct tccgctgggt
    19621 acattgcgcc agccgggccc ggccttcatt cctatcctac tggcttctct tcttctgatc
    19681 ttcgcaattc ttctggtact aaccagcagc cgcgctccat cgttatcatc cgttaagtgg
    19741 accgaatggc gccatgcgct ggccattttg gcagcgtccc tcttttcggt tttcgccatc
    19801 gagcgtctcg gttatcgtct cactgtgctg ctcgtcctgg gctttctggt caaactggtg
    19861 gaacagcgtg gttggatagt ttccttatcg tttgccttca ccttgtcttt tgcttccttc
    19921 ttccttttct ataccgtcct gcgagtgccc ctgcctcagg ggccattcgg attctgaaag
    19981 agccaactca tcttgagcaa cgattcattc ggatggcgca gtagaaaggg agagctggac
    20041 agcgatggca agccttgaaa gaagcgacgg cctagagaac gaatcggtac cagccgacga
    20101 gcgcttggtc aggactcgca cgttcggagt agctgcgacg tagatggcga cgccacgacc
    20161 gctggccgag tgtcttttgc tatcggaggg ctcaccgctt gatccaaatg acacttcgaa
    20221 cccggaatcc cggtttgcag tgagttgatc ctgtcatgac tgaaactctg cacaatcttt
    20281 acgtcggctt ttcagtcgcc cttgcgccca acgttttact ctacgccttt gtcggctgtg
    20341 taatcggaac cctcgtaggt gtcttgccag gtgtgggtcc gcttgccgga atcagcttgc
    20401 tcctgcctgt ttcgttcggt ctcgatgcca cttcggcgat cgtgcttctg gccggaatct
    20461 actacggcgc catgtacgga gggtccacca cttcaatact gatgcgcatt ccgggtgagg
    20521 cagcttcggt catgacctgt atcgacggtt acgagatgac gcgcaaagga cgggccggcg
    20581 ccgctttggc cattgcagcg gttggttctt tcatagccgg aaccgtcagc atcgttggcc
    20641 tcatgctttt ggcgccgccg ttggcgagct ttgcgctccg cttcggacct cctgaatatt
    20701 tcactcttct gcttctcggc ctcctggctt tggcctatat gagcggcggc tccatgctca
    20761 aatcgctggc gatggcaagc ttggggttgc ttatcgggat gatcggcatc gatcccatga
    20821 ctggattcat tcgctttagc tatggattga tcgaactcgg tgatggcatc ggggttgttc
    20881 cagtcgcagt ggggctattc ggaatctcag agattttgct caccgcagga cagccctcca
    20941 ccccccgagt caagcagccc aggctcaaag aacttctgcc ctcgcgcgct gaatggcgcg
    21001 attcattctg gccgttcggt agagggaccg ttctgggttt tttgatcggc atcataccgg
    21061 gctctgctca cattatcgcc tcgttcgttt cgtatgcgat ggagcggcgc ctttccaagc
    21121 atcccgaacg cttcggtcat ggcgccgtcg agggagtagc ggggccggag tcggccaaca
    21181 acgcggcgac atcaggagcc tttgttccta tgctcgcctt gggagttccg tcggggccga
    21241 tcccggccgt gatgattgcg gctatgatgg tgcatggcat ttcgccgggt cctcttttga
    21301 ttgcccagca accggagctc ttttggggtt tcgtcgccag catgtacgtt ggcaatctcg
    21361 tgctgttgat tttgaatctg ccgctggtcg ggatttttgt caccatttta aga
//
LOCUS       DASUVS010000173         9998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_286211, whole genome shotgun sequence.
ACCESSION   DASUVS010000173 DASUVS010000000
VERSION     DASUVS010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9998
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286211"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1301)
                     /locus_tag="VF182_09580"
     CDS             complement(165..1301)
                     /locus_tag="VF182_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437368.1"
                     /translation="MVQIHSTALTEPLALFLGFLGLYLLARYINGAKLVLLYISALSI
                     ALSSLTRYAAVIFVLTGIGALIWLNPWSWKTKSIRSSLFLVLSALPLIAWVARNTLSA
                     GNAVNRTFAVHLPKPEDLTTALDAVSLWVFPVTLWREAVWPRLLVLLFVVAVLSWSAA
                     KMVLLKSRLHEICALFVVCYVAFVFASRSLLDAAIPFDTRLLAPAYFAAMILIVSAVW
                     VERTRPLKSMSLLRRIAVYVVFLLSVLQAIPAMAWLKISYTSGLGLAAKGWKNSESMR
                     IVGKLDATTPVYTNGPDLIYMFLNRLSYMIPRKLDPYSQLPNGQYEIEVAEMKKILRE
                     KNGVVAYFDAESREWFLPSLKQLDMKIGLRMISGESDPVIYRIK"
     gene            complement(1338..1556)
                     /locus_tag="VF182_09585"
     CDS             complement(1338..1556)
                     /locus_tag="VF182_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437369.1"
                     /translation="MLRGEGVTYFDDNGQMAPVTHYAPLYPLMVAGLGVAGIDPLEGV
                     RWLNALLFASNIMLASWIVFTSTRPVAA"
     gene            complement(1751..2677)
                     /locus_tag="VF182_09590"
     CDS             complement(1751..2677)
                     /locus_tag="VF182_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6437370.1"
                     /translation="MPFTRSVSRSCAVSIIIPVFNEAESLREVLEDIRALEIAQSEIL
                     VIDDGSLDGSAEIAFNAGANVVRHPYNIGNGAAVKSGIRAAQGRRLVLMDGDGQHQPA
                     DIPKLLAQCERYHMVVGARGKGSKLRFHRYAANLFYNQLASYVTRFKVKDLTSGFRVL
                     SRSDALRFIDLLPNTFSYPTTLTLAFLRSGLTIKYVPIQSLYRSGQSKISLVTDGIRF
                     LLIITKIATLFSPFRIFLPVSLFFFFAGLANYIHTYLTQYRFTNMSVFLLTTAVIIFM
                     LGLVSEQIALLRMEQRASFRSERTEGRYDQEL"
     gene            complement(2652..4436)
                     /locus_tag="VF182_09595"
     CDS             complement(2652..4436)
                     /locus_tag="VF182_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX6437371.1"
                     /translation="MIDERSSALLFRTCLAGCVLGTAATVYLLVGLAGHVHNPLANVA
                     VVSVLATSALFFLVEFFSIPIKSNRLERSIWFAILVILIAEIVLGFLPPTARDELTHH
                     LAIPKLYARAGQIIEVPMAPYAYYPMLLDMLYTPFVYWGYDFIAKHTHALFGFLTGLL
                     IYAYITRRLSRVYGLLGFFFFISTPAVLRLSHWAYIDLGITFYSTAALLCLLRWSEER
                     EARCWLFLAGLSLGFALATKPNGLLAALLLGLLFVLAIGRGPRKPVGRLVSELALFVG
                     VTLLPFTPWLMKNWHQTGNPFYPSLGSLFPPRSASFIDPASSVGFGVLEKRHLLYGEN
                     LWQIIGLPLRIFVSGQDDKPQYFDGVLTPVLLLFLPWAFRGKWSEEKKLIAFFAFGYL
                     ALALFLIDMRIRYILPIVPALVVLLGYGIFNMYLSIKRPILLYAALLLFAAWHGTYLW
                     RYVQDSRSYNYLRGTESREAFLNRALSEYPAMRYINQKLTGTAKIYLLFLGRRAYHCE
                     RDYFHDAGELPAFLIGAIQKAKDAGDIGRVLDSRNLTHLMIREDLLIRFLTENLTPAQ
                     GRMWNAFATTRLKLAFRERGYAVYEIRL"
     gene            complement(4801..5400)
                     /locus_tag="VF182_09600"
     CDS             complement(4801..5400)
                     /locus_tag="VF182_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6437372.1"
                     /translation="MAVRLFQSQTSMFCEKVRVVFAMKKVPYEIVDVRKDDRKSLIEY
                     TGQRKVPSVDYNGKCVIDSTIISALLEDQQPAPSIFPEGAANKGLCLALEDWSDEVLI
                     HANHAIRRAETPEARKKAEDEWAGHFRTLDLMYSGKKFIFDRITIADIAIFSQLHYLY
                     TTVKYEIPASYKNVHAFLENMRQTLKLNALADSFEAKSL"
     gene            5592..6674
                     /locus_tag="VF182_09605"
     CDS             5592..6674
                     /locus_tag="VF182_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6437373.1"
                     /translation="MSAAESDQKENFYQEAEDKHLIPLWKVTAKLLPPQPQTKVLPYL
                     WKWSDLRRMAYRAGELVPIERGGERRVLAFVNPGLQGKYAATHTLWGAVQIVLPGEIA
                     PCHRHSASAIRFIIEGGRSFTNVDGDKCVMSRGDLVLTPNWTWHDHGNESDQAMIWMD
                     GLDLPLVNDLDGIFFEPYPQHKHPVTQVNGSEQKYGGAHLKPTWENPPATRSPLLNYK
                     WGPTYEALKRIGEGPASPFDDVCFEYLNPNTGGPTLPTMSCCVQLIRPGVHTQAHRQV
                     NSAVYHVFEGRGHAVINGQRFDWQRGDFFVVPPWAWHEFANESKEEVILFSVQDTPVM
                     QALGLYKEEAYSQNGGRQEVTATFSG"
     gene            6693..6902
                     /locus_tag="VF182_09610"
     CDS             6693..6902
                     /locus_tag="VF182_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437374.1"
                     /translation="MAKYLQCSECGKLAFYYDDYVGTEKPFYKRFMRYPEGETTYQLP
                     RCFSCGGVPRMIHDLIKEEKAAAKH"
     gene            7741..9030
                     /locus_tag="VF182_09615"
     CDS             7741..9030
                     /locus_tag="VF182_09615"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HEX6437375.1"
                     /translation="MPKMRILWDLFTSARGPLPTERDLRLDFFRGLALFSIFICHIPG
                     NILAWFTLQAVMCADAAEVFVFVSGFTAGTVYSRTMRREGFLIAGLRVYRRVWQLYIT
                     NIVLFVTLIAIISSTADLLKTSRYIDYFIGTPDFFQEPGVALARVLSLQFQPGFIDIL
                     PLYIVCLGFLPVVLAGFRFRPGLVILTSLSLWVTVQLDGRIAPPAWPSAATWGFNPFA
                     WQALFYLGTWLGWRGNQPEVFYLGRRWRIYLAAGLVMAGFLIRFNWTLQGFYGPIKSP
                     IPGEPLWQFLDKTNLGLIRFVNIFAIALLVGHMIHPQGGFFCSRIARPFILCGRHSLP
                     IFCLSAVLAVFGQLILNEIFGRWPMQLAVSTTGVAIMVGLAAMLDWFAATRNPSRGRS
                     LEIRRQVAEPNMNQVPMWSAAEVKTAAAHGPQIREQQ"
BASE COUNT         2549 a         2618 c         2591 g         2240 t
ORIGIN      
        1 atcgagagtc aaacccaaaa cggaaaagta ttcggccacg gttataactc ttcgggaccg
       61 cacggatgcc cgccgtgcgc tgccgagcga tctcatcaga gaaggcaatc tgcaacaact
      121 aatcaccacg ttgaaactgc gccgtggctg tttgccacgc ttatctactt gatacggtaa
      181 atgaccggat cgctttcgcc tgagatcatt cgtaagccga tcttcatgtc caactgtttc
      241 aacgacggca gaaaccactc cctactttcc gcatcgaagt acgccacaac cccgttcttc
      301 tcccgtagaa tttttttcat ctccgcgact tcaatctcat attggccatt tggcagttga
      361 ctataagggt ccagcttgcg tggaatcata tagctaagcc tgttgagaaa catgtaaatc
      421 aaatctgggc cattggtata gacaggagtt gttgcgtcca gcttcccaac aattcgcatc
      481 gattcggagt tcttccaacc tttagctgcc agaccgagac cagaagtgta gctgatctta
      541 agccacgcca ttgccggaat tgcttgtaag accgacaaca ggaaaacaac gtagacagcg
      601 atgcgacgaa gcagggacat acttttcagt ggtctcgtcc tttcaaccca aactgccgat
      661 acgataagaa tcatcgctgc aaaatacgcc ggcgctagca ggcgagtatc gaaggggatc
      721 gctgcgtcaa gaagtgaccg cgacgcaaat acaaacgcaa catagcaaac aacaaacaac
      781 gcgcagattt cgtgtaagcg cgatttcagc agaaccatct tagctgcaga ccacgataac
      841 accgcgacga cgaacagcag aaccaatagc cgcggccaaa ccgcctcgcg ccacaaggtt
      901 accggaaaaa cccaaagtga aaccgcgtca agcgcggtgg tcagatcttc gggcttaggc
      961 aggtgaacgg caaaggttcg gttgacggcg tttccggctg acagagtatt gcgggcaacc
     1021 caggcgatca ggggcagcgc gcttaaaact aaaaagagac ttgagcgaat cgatttcgtt
     1081 ttccaactcc agggattgag ccagataagt gcgccgatcc ccgttaaaac gaaaatcact
     1141 gcggcataac gtgtaaggct actcagagct atcgaaaggg cagagatata gagtaggacc
     1201 agtttggcac cgtttatata ccgggctaac aaatagagac cgaggaaccc taaaaacaag
     1261 gccaagggct cagttagcgc cgttgagtgt atttggacca taggaaacgc cgtagtcgcg
     1321 aagaaagaag cggccaatca tgcagcaacc gggcgagtgg acgtgaacac gatccaagat
     1381 gcgagcatga tattcgacgc gaacaacagc gcattcagcc aacggacgcc ttccagggga
     1441 tcgatgccgg caactccgag acctgcaacc atcaacggat aaagaggcgc atagtgcgtc
     1501 acgggcgcca tctggccgtt gtcgtcgaag taggtcacgc cctcaccccg taacacattg
     1561 cgagcaactc ctacataaac gaccgaatcg gaataaattc caattccacc ggggggtgcg
     1621 taggataact cccaggaaac cgatagcagc gaggccgggc acaatcgcgc aaaggaaacg
     1681 gcggttgcag caaatcatga ggcgacctta gtgtagtgat acaaggaaca actgccaagg
     1741 gtggcaattt ctatagctct tgatcgtatc ggccttctgt acgctccgac cggaacgatg
     1801 ctctttgctc catcctcaga agcgcgatct gctcagacac caagcctagc ataaaaatga
     1861 tcaccgcggt ggtcagcaga aaaacagaca tgttagtgaa acgatactga gtcaagtagg
     1921 tatgaatgta attagccaac cccgcgaaga agaaaaacag actgaccggc agaaagatac
     1981 gaaaaggcga aaacagagtg gcgatttttg tgataatcaa caagaaccgg ataccatcgg
     2041 tgacaagact aatcttgctt tgcccggagc gatagagtga ctgaatcggc acatacttga
     2101 tggtcaaccc agagcgcaaa aatgccaagg tcaaggtcgt cggataggaa aaggtattgg
     2161 gcaaaagatc gatgaaccgc aaagcatcgc ttcgcgacag gacgcggaaa cctgaggtaa
     2221 gatcctttac tttgaaacgg gtcacataag aagccaattg attgtaaaag aggttggccg
     2281 cgtagcgatg gaagcgcagt tttgagccct tgcctcgcgc gccgacgacc atgtggtacc
     2341 gttcgcactg agccaataat ttcgggatgt ccgccggttg gtgctggccg tctccgtcca
     2401 tcaacaccag tcttctcccc tgggcagctc tgataccgct ctttaccgcg gcaccattgc
     2461 cgatattata aggatggcgc accacattgg cgccggcgtt aaacgctatc tcagcgctac
     2521 catcaaggga cccatcatcg ataaccagaa tttcagattg agcgatttcc agcgcgcgga
     2581 tatcttccaa aacttcccgc agactctcgg cttcattgaa aacaggaata ataatcgata
     2641 cagcgcacga tctagagacg gatctcgtaa acggcataac ctctctctcg gaaagctaat
     2701 ttaagccgcg tcgtcgcgaa agcattccac atccggcctt gagccggcgt tagattctcg
     2761 gtgagaaagc ggatcaggag atcctcacga atcattagat gcgtcagatt cctgctatcg
     2821 aggactcgtc cgatatctcc tgcgtccttt gctttttgaa tcgctccgat aagaaaggca
     2881 gggagctcgc cggcgtcatg aaaataatcc cgctcacaat gatacgccct gcggccgaga
     2941 aatagaagat aaatcttagc cgtgcccgta agtttttgat tgatgtagcg catggccgga
     3001 tactccgaca gagcccgatt gagaaacgct tcgcgagatt cagttcccct gaggtagttg
     3061 tacgatcggc tatcctgtac ataccgccag agataagttc catgccaggc ggcgaaaaga
     3121 agtagcgcag cgtagagtaa aatcggccgc ttgatgctga gatacatatt gaagatgcca
     3181 tagcctaata atacgaccaa cgccggcacg atcggaagaa tgtagcggat gcgcatgtcg
     3241 atcagaaaca aggccaaggc caagtagccg aatgcaaaga acgcaatcag ctttttttct
     3301 tcactccatt ttcctctgaa agcccaaggc aggaacaaca gaagtaccgg tgtcagcacg
     3361 ccgtcaaagt actgtggctt gtcatcctga ccagagacaa aaattcgcag cggcaagccg
     3421 ataatctgcc acagattctc gccgtatagc agatgtcttt tttctaagac cccaaagcca
     3481 acggagcttg cgggatcgat gaaagatgcg ctccgggggg ggaaaaggct gcctaatgaa
     3541 ggataaaaag gattgccggt ctggtgccag tttttcatca accacggcgt aaacggcaac
     3601 aacgtgacgc cgacgaacag ggcgagctcg gaaaccagtc tgcctaccgg cttcctcgga
     3661 cctcttccaa tggccagcac aaacagcagc cccagcagca gggcggcaag cagcccgttg
     3721 ggtttcgtcg ccagcgcaaa cccgagcgac aagccggcga gaaatagcca acatcgagcc
     3781 tccctctcct cagaccagcg gagcaagcat aaaagagccg cagtggaata gaacgtgata
     3841 ccgagatcga tgtaagccca gtggctcaac cgcaagacgg cgggggttga aatgaaaaag
     3901 aaaaagccca gcaacccgta cacccggcta aggcgccgcg ttatataagc atagatcaac
     3961 aagccagtaa gaaaaccgaa gagcgcgtga gtgtgtttgg caatgaaatc gtagccccaa
     4021 taaacaaacg gtgtatagag catatcgagg agcatgggat aataggcgta gggcgccatc
     4081 ggcacctcga tgatttgtcc cgcgcgcgca tagagttttg gaatcgccag atgatgggtt
     4141 agctcatcgc gggccgtggg cggcagaaag ccaagaacga tctcggcgat gaggatgacc
     4201 aggattgcaa accagatgga tctctccagc cggttcgatt tgatcgggat tgaaaagaat
     4261 tcgactaaga aaaacagcgc gctggtggcc agaaccgata cgacggcaac attggccagc
     4321 ggattgtgca catgaccggc aaggcccacc agcaagtaaa cggtcgcagc cgttcccaga
     4381 acacaaccgg ctaagcaggt tctaaacagg agggcagaag agcgctcgtc aatcattcag
     4441 atgccaacct gcgaagcggg actggtttag ggcgaagggt tgcaagctaa gcgcccacca
     4501 gaacggaatg tctggtgagc gcatcagcgg atagagaaaa cgggttgagc ttgaatgcgg
     4561 caagtcatgg gcccgatctg ggctcgatgc cgggaataac gagcaagata ctaggaattg
     4621 ccatcctttg cgctcgtgtc tcgccattat tcagcgcagg agattttatc atcctaattt
     4681 aaattttcgc ctgatctcga tcgatccggc gcttcctgcg cgcgctgaga attacttcgg
     4741 ccatcgcagt attttaattc ccagaatcac cgcgaggatc acatcttttt acataacatc
     4801 ttagagcgac ttagcctcga agctgtccgc cagtgcgttc aatttcagag tctggcgcat
     4861 gttttccaag aatgcatgaa cgttcttata actagccggg atctcatatt tgaccgtagt
     4921 atagagataa tggagctgcg agaaaatcgc gatgtcggcg atggtgattc gatcaaaaat
     4981 aaatttcttg ccggaataca ttagatccaa ggtcctgaaa tggcccgccc actcgtcttc
     5041 ggctttcttg cgagcctccg gtgtttcggc ccgtcgtatc gcgtggttgg catgaatgag
     5101 gacttcatcg gaccagtcct ccagagcaag gcaaagaccc ttgtttgccg ctccctccgg
     5161 aaagatgctt ggcgcaggct gctggtcttc caacaaagca gagatgatcg tcgagtcgat
     5221 cacgcattta ccgttataat caacggacgg aacttttctt tgcccggtat actcgatgag
     5281 cgacttgcga tcgtccttgc gcacgtcgac aatctcataa ggaactttct tcatggcaaa
     5341 aacaacgcgc accttttcgc agaacataga tgtttgtgat tgaaaaagcc tcaccgccat
     5401 aatttattct cccttcgatt aaatagtcgg gttgtaaacc gtctagggct gttgacggaa
     5461 aaccgaaatc gattctagaa gccttttagc agccatgcaa cagatttcgc gttgctcagt
     5521 cgcggcgctg cgcagtcatt gacctgctga gtattcatga gtattcttca agacgtaaag
     5581 aggacgcccc tatgagtgcc gctgagtcag accaaaaaga aaatttctac caagaagccg
     5641 aggacaagca tctcataccg ctgtggaagg tcacggccaa attgctaccg ccgcagccgc
     5701 aaaccaaggt cctgccttat ctttggaagt ggtccgatct gcgacggatg gcgtatcgcg
     5761 ccggcgaact tgtcccgatc gagcgcggcg gggagagacg agttttggct tttgtgaacc
     5821 cgggtctgca aggtaagtat gccgctacgc ataccttgtg gggggcggtt cagatcgttc
     5881 ttccaggaga aatcgcgccg tgtcatcgtc attcggcatc ggcaatccgg tttatcatcg
     5941 agggcggtcg ctcgttcacc aatgtcgatg gcgacaagtg cgttatgagc cgcggcgatt
     6001 tggtgctgac tccgaactgg acctggcacg atcacggcaa tgaatctgat caggcgatga
     6061 tctggatgga tggcctcgat cttccgttgg tcaacgactt ggacggcatc tttttcgagc
     6121 cttacccgca gcacaaacat ccggttaccc aggtgaacgg atcggagcag aaatatggcg
     6181 gcgctcattt aaaacccacc tgggagaatc cgccggcaac tcgttctcca ttattgaact
     6241 acaaatgggg cccaacttac gaggccctca aacgaatcgg tgaaggtcca gccagtccgt
     6301 tcgatgacgt ctgtttcgaa tatttaaacc caaataccgg tgggccgact ttgccgacta
     6361 tgagttgttg tgttcagctg attaggcccg gcgttcacac ccaagcgcat cgacaggtca
     6421 atagcgccgt ctatcacgtc ttcgaggggc gcggccacgc agtcatcaat ggccagcgct
     6481 ttgattggca acgcggcgat ttctttgtgg tgccgccttg ggcgtggcat gaatttgcca
     6541 acgagagcaa agaggaagtg attctcttct cggtgcagga cacgccggtg atgcaagccc
     6601 tcgggcttta caaagaggaa gcctatagcc aaaacggcgg gcggcaagag gttaccgcaa
     6661 ccttcagcgg ttaggactta ttagggactg gcatggcaaa gtatttacaa tgcagcgagt
     6721 gcggcaagct ggccttttac tatgacgact atgtcggcac tgagaagccc ttctacaagc
     6781 gattcatgcg ctaccctgaa ggcgaaacca cttaccagct tccccgctgc tttagctgcg
     6841 gcggcgtgcc acggatgatc catgatctga tcaaggaaga aaaagccgcc gccaagcatt
     6901 agacgaagca gctccgtcgt cgagaacgtt atctttcgcg ttttagagca gagaagtgca
     6961 caaaaaggag gcggctgatt acccgcccct ttttttagac tcgtctaccc tgcaacagat
     7021 ctcattcacc ttaaagagcc gctcgcgttg ggcccaagcg atatatatag cccctccgca
     7081 atctgatagc cgccgatcga aaaaagcccg gctggagact ttcttcagcc cgaatagtag
     7141 gacacggaga tgcgcgattg tttatatttt ggatggcata caatcggacg atatttgtcc
     7201 gcacatgctt acttaatagt atttggaagt ttcaggttgc ctaagaattt tgtcgcttgc
     7261 tgaacgaggc caaaattcgg ccttctaaca gtgcagcgta gctcagaact cgaggctgag
     7321 ccacaatgat tgaagcggct gatggatgtc gccgaagaga tgagcgacaa agcgcagtgc
     7381 cgtccgcttc tcgtttcccg gcgcgtcggt tccaaaggac tcagctgtac gaatgagcca
     7441 taatcttgct gcggcatctg gcaataaagg tactcctttc ctcgcgccaa tcatgatgca
     7501 actcttcgat cgcccgcctg aggtcagatt agcgcagtgg tcgttaggtt aaagggcata
     7561 atctacatag tgggatgcaa cggttaatag ggtgcactcg ggctaaataa cccttttccc
     7621 ccaaacctcg ggcttttccc gctacagaat tgtcgtgttt tgagcggcac gttcccgcac
     7681 tgtcctgcga aaccatggca cattcttggc gtttcgggtt tcaccgggaa gtccgcacgt
     7741 atgccaaaaa tgcgcatctt gtgggatctc ttcaccagcg caagaggtcc tttgcccacc
     7801 gaacgcgatc tccggctcga ttttttccgt ggccttgctc ttttctccat ctttatttgt
     7861 cacatcccgg gcaatatctt ggcgtggttc acactccagg cagtgatgtg cgctgacgcc
     7921 gctgaggtct tcgtttttgt ttccggcttc accgcaggca cagtgtacag tcgcacgatg
     7981 cggcgcgagg gttttttgat agcgggactt cgggtgtacc gccgcgtttg gcagctctac
     8041 attaccaaca tagttctgtt cgtaaccctt atagcgatca tttcgtccac cgccgatctc
     8101 ctcaagactt cgaggtacat cgattatttc attggcacgc cagatttttt ccaggagccg
     8161 ggcgttgcgc tggcgagagt attgtcgttg caatttcagc caggcttcat cgatatccta
     8221 ccgctttaca ttgtgtgtct cggattcctt cctgtagtcc tcgccggatt ccggttccgg
     8281 ccggggctgg tgattcttac gtccttatca ctctgggtca cggtgcagct cgatggccga
     8341 attgcaccgc cagcatggcc tagcgcagca acctggggtt tcaacccgtt cgcatggcaa
     8401 gcgctattct atctgggcac ctggctaggt tggcgcggta accagcctga ggttttctac
     8461 cttggccgta gatggcggat ctaccttgcc gcagggttgg tgatggccgg attcttgatt
     8521 cggttcaatt ggacattgca aggcttctat ggtccaataa aaagtcctat ccctggcgag
     8581 ccactgtggc agttcttgga taagacgaat ctaggattaa ttcgtttcgt gaacatattt
     8641 gccatagcgt tgctcgtagg acatatgatt catccccagg gcgggttctt ctgtagtcga
     8701 atcgcgcgcc cttttattct ctgcgggcgt cactcgctcc ctatcttctg tctcagtgct
     8761 gtactcgcgg tgttcggaca gctgatacta aacgagatct tcggccgatg gccaatgcag
     8821 ctggcggtct ctacaacggg cgtcgcaatt atggttggtt tggcggcgat gctcgattgg
     8881 tttgccgcta cccgaaaccc gtctcgcggc agatcgttgg agatccgacg gcaggtagct
     8941 gagcctaaca tgaaccaagt gcccatgtgg tccgcagcag aagtgaaaac cgcagctgct
     9001 catggcccgc aaatacgcga acagcagtag ctgcctcacg ctcccggtaa atagtttgcc
     9061 tagaggctgg ctataaaccc gtgcactgga ctagaggtca aggctgcttg cgagattcaa
     9121 ctttaggaag tgtgaacgga tgaggcacaa cacaacgagg acacgcatcg gcagaccggt
     9181 tgattcgtgc tgcgcaacga acgcaaaccc ttttacgcaa tcgcctcaga agaagtaagg
     9241 cgatcatgac tgccgctact gcgacatatt cgtttatgtg agggccggta atctgtatcc
     9301 caatttgtgg aaaccaatgc agcatcacga cgactgccaa gtgcatggcc gagcgcacct
     9361 acagtacggt gatgataaag tatgcagcag cgaggtaagt ctcaatgtac cgaagactac
     9421 taacaaaata agtaaagtgt cttcgacacg gctcgaccag aacttacggg ctttgatcat
     9481 atgagtatct ctcacttcgc tgatgatctt tttaagccgg tagttccttt gcctttaaca
     9541 tttctcggat ctgagctgaa gatccagacg ttaaacggct tgacaagaca gccgttactt
     9601 ccggtccaaa cacgccttca atcctcagaa cgaagactgc ggttgtgcca agatcgggat
     9661 gtttttgttt cagaggcgcg caagtgttct cgccgcttcc aaccgaccat cacccgcata
     9721 tcattgtcca tcaagatcaa gtcctgcctc tctgcgttag cctttctacg ccctctttac
     9781 catggctgtg agaatgggat atagccagct agccgtactc tgagcgctaa taggacgcga
     9841 tatcagggtt atcttccacg atcaggattc tcgttcgcct acctttgtcg aatcatcatc
     9901 aaccaactaa caaagagaat ggcgccggga acgaaaagaa tctttggtgt agttacgagt
     9961 tgcgcttctt cgccccggcc actagccgac tgggcaag
//
LOCUS       DASUVS010000174         3112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1161167, whole genome shotgun sequence.
ACCESSION   DASUVS010000174 DASUVS010000000
VERSION     DASUVS010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161167"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="VF182_09620"
     CDS             <1..1359
                     /locus_tag="VF182_09620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6437376.1"
                     /translation="DLTADTIPYFQRDKEYVYAPDRSIWILVSTALLHDKDRRPLNLI
                     SHIQDISKYKEVEKEKQTFAHQLSERVKELKALHYIARLLHDDEKPTSILLQEIVTFL
                     PAAWEYAERAAARIAFDGAEYKSQNFIAMGGTQSADFVTAGGKHGLLEITYPETGADQ
                     AELGFLAEEKNLLDSLAEMLKLHLDRKEARDRIDRITRELVERNKELWSLQQEMGRVE
                     QRVALGWMTGAIAHELGTPLNSVLGYTHLLAQEDLSEKAQRHVKTITSQVQRMTSIVQ
                     YYLDRTRGSTSERSWVNINDLIRETLLLLDSVFAQRNVHPVMNLEESLPLINAHGGSL
                     QRVLINLLNNALASIRDKVEITIATRTELAQRQRPGIIPGIIIEISDTGVGIPADLLP
                     KIFDLFMTTRPEGSGTGLGLAVSQEIVKEHGGKITIDSQVERGTTVTVFLPTNFDRMG
                     QL"
     gene            1356..2798
                     /locus_tag="VF182_09625"
     CDS             1356..2798
                     /locus_tag="VF182_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6437377.1"
                     /translation="MKARILVVDDDKVSCQLFTEVLEAEGHEIVNAYSGEEALERLRS
                     EPYDLLLVDVRMPGITGLEVTRIMRQEQPQLPIIVMTAFGSIETAVEAIQEGAVDYVS
                     KPMNLDELKRIVSRAVGQRELGAFSRSKLKQLEDPEQQKTIIGRSAAMVEVFKRVARV
                     ASTKSTVLILGESGTGKEVIARSIHRHSDRAQRPFVAVDCGALTETLLESELFGHTRG
                     AFTGAVSDKKGVFQLASAGTCFLDEIGDISANMQAKLLRVLQEGEVRPVGAKEWLKVD
                     VRVLAATNKDLTDLVKRGVFREDLYYRLNVVTIQLPPLRERPEDIDALLEIFVRRYSE
                     LARKHITAISHDALERLKTYSWPGNIRQLENAIEQAVVLSTHPVITLEDLPREIREGV
                     APSYSNAEDQVLVVSDMPSLDEIKKRYVVHVINRVRGNISRAAKTLNVDRRSLYRMLA
                     RWKVVPFAAVRSEEPLENGSKDAKSTDEAK"
     gene            2867..>3112
                     /locus_tag="VF182_09630"
     CDS             2867..>3112
                     /locus_tag="VF182_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6437378.1"
                     /translation="MRVLIVEDDETFCAFLMQILEGQGHEVVCATDSLEGYQKAWSYS
                     YELIILDVRMPGLLGTEIAAALKQKNPSAKIILISAFA"
BASE COUNT          784 a          811 c          878 g          639 t
ORIGIN      
        1 gacctgaccg ccgacacaat tccatatttt caaagagata aggagtacgt ctatgcgccg
       61 gatcgttcga tctggattct tgtgagcact gctttgctcc acgacaaaga ccgaaggcca
      121 ttaaacttga tcagccacat acaggacatc agcaagtaca aggaggttga aaaagaaaaa
      181 caaacgtttg cgcaccagct cagtgaacgg gtcaaagaac ttaaggcgtt gcactatata
      241 gcgcgattgc tccatgatga cgagaaacca acgtcgatct tgttgcagga aatcgttacg
      301 tttcttccgg cagcctggga atacgccgag cgcgctgcgg cacgcatcgc ttttgacggt
      361 gcggaatata aaagccaaaa cttcatagcg atgggcggaa cacaaagcgc agatttcgtg
      421 accgcaggag gcaaacacgg cctgttggaa atcacctatc cagaaaccgg agcggatcaa
      481 gcggagcttg gttttctcgc tgaggaaaaa aatctgctcg attcccttgc agagatgcta
      541 aagctccatt tagaccgcaa ggaggcgcgg gatagaatag atcgaatcac ccgagaattg
      601 gttgagcgca ataaagaact ctggtccctg cagcaagaaa tggggcgagt ggaacagcgc
      661 gtcgccctag gttggatgac cggagccatt gcccacgaac tcgggactcc attaaattcg
      721 gtgctcggat atacgcacct gttagctcag gaggacctgt cggagaaagc ccagcggcat
      781 gtgaaaacaa taaccagtca ggtccagcgc atgaccagca tcgttcaata ttatcttgat
      841 cgcacgcgcg gctccacgag tgagcgcagc tgggttaaca tcaacgattt gatcagggaa
      901 actctccttt tgctcgactc ggttttcgcg caaaggaatg ttcacccggt catgaatctc
      961 gaggaatcgt tgcccttgat caacgcacat gggggctcgc tgcagcgtgt tctcatcaat
     1021 ctcctcaaca atgccttggc ttcgatccgg gataaagtcg aaatcaccat tgccactcga
     1081 actgagctcg cgcaacgtca gcgcccgggt attattcccg gtatcataat cgaaatcagc
     1141 gacaccggtg tgggcatccc tgcagattta ctgcctaaga tttttgatct tttcatgacc
     1201 acccggcctg agggaagcgg aaccggatta gggctggcgg tgtcgcagga gattgtcaaa
     1261 gagcatggag gcaagataac gattgatagt caagtcgagc gcggcacgac ggtaacggtg
     1321 tttctgccta ccaactttga tcgcatgggg caattgtgaa agcgcgcatc ctggtagttg
     1381 acgacgataa ggtaagctgc cagcttttca ctgaagttct cgaggcagag ggtcatgaga
     1441 ttgtcaacgc gtatagcggg gaagaggcgc tcgagcgcct gcgcagcgaa ccttacgatc
     1501 tcctgctggt cgatgtccga atgccgggaa tcaccggact agaggtcacc cggatcatgc
     1561 ggcaggagca gccgcaactc cctatcatcg taatgaccgc ctttggctcg atagaaacgg
     1621 ccgtagaggc tatccaagaa ggcgccgtcg actatgtctc aaagcccatg aacctcgacg
     1681 agctgaaaag aatcgtctcc cgagccgtgg gacagaggga attgggtgcg ttttcccgga
     1741 gcaagctcaa acagctcgaa gacccggagc agcagaaaac cattattggc cgaagcgccg
     1801 ccatggtgga ggtgttcaag agagtggccc gtgtcgcttc gaccaaaagc accgtgctga
     1861 ttctcggcga aagcggcacc ggcaaggagg tcatcgcccg ttcaatccac cggcacagtg
     1921 accgcgcgca aaggccgttc gtggctgtgg actgcggggc actgacggag acgctgcttg
     1981 aaagcgagct gttcgggcat acgcgcggcg cctttaccgg agcggtatcc gacaagaagg
     2041 gagtttttca gctcgccagc gccggcacct gttttctcga cgagatcggc gatatcagcg
     2101 ccaatatgca ggccaaacta ctcagggtct tacaggaagg tgaagtgcgg ccggtcggcg
     2161 caaaggaatg gctgaaagtc gatgtgcggg tattggccgc gaccaataaa gatttgacgg
     2221 acctggttaa gcgtggggta tttcgcgagg atctctacta tcgtttgaac gtggtgacaa
     2281 tccagcttcc accactacgc gagcggcccg aagacatcga cgcgctgttg gaaatttttg
     2341 tccgccgcta cagcgaacta gcgcgcaagc acatcaccgc gatctcacac gatgctctcg
     2401 agcgtctgaa aacctattcc tggccgggca acatccggca gcttgaaaat gccatcgagc
     2461 aagccgtggt gctctcaacg cacccggtaa tcacgctcga ggatctgccg cgagaaatac
     2521 gcgagggtgt ggccccgtcc tatagcaacg ccgaagacca agtcctcgtc gtttcagata
     2581 tgccgagcct cgatgagatt aaaaaacgtt acgtcgtgca tgtcatcaac cgtgtccgtg
     2641 gaaatatttc gcgggcggca aagactctca acgtcgatcg acgttcgctc taccgcatgc
     2701 tggcgcgatg gaaggtagtc ccgtttgcgg ctgtcagatc cgaagagcct ctggagaacg
     2761 gcagcaagga tgcgaaatca accgacgaag caaaatagcc tcccgaccgt ttgcgagcgg
     2821 catgtgcatt gcacagcaac cgcatgagag gagaacggag gcttcagtgc gtgttttaat
     2881 cgtagaggac gacgagacat tctgtgcatt cctcatgcaa atattagagg gccaggggca
     2941 tgaggtggtg tgtgctacag attcgctgga ggggtatcaa aaagcgtggt cctattccta
     3001 tgagctgatt attcttgatg tgcgtatgcc gggactctta ggcaccgaga ttgccgcggc
     3061 gctaaagcag aaaaatccct cagccaaaat catcctgatc tcggccttcg cc
//
LOCUS       DASUVS010000175        19959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_940881, whole genome shotgun sequence.
ACCESSION   DASUVS010000175 DASUVS010000000
VERSION     DASUVS010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 19959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19959
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940881"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..1559
                     /locus_tag="VF182_09635"
     CDS             345..1559
                     /locus_tag="VF182_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6437379.1"
                     /translation="MKNPRVIIAGGGPVGVVSALACAQHGYPVTLLEAEDKIDDNPRA
                     ATTHPSTLELIARVGLIDQFIREGLVARFFQFWDKPSRTKIVEFDHDILRDETPYPFV
                     VQTEQHKLARMGIERLRTFPDAEVRFSSRATTVDQDADGATVTIEGSQGTETLRGEYV
                     IGADGGRSTIRKALDIEFEGFTWPERFLVLTTIEDFQKLLTGCCYRNYLADPDEWTNL
                     FKVAGDDGKGRWRAVFPTRVEESDEQALGDDSTYSRLQRVFPMERPYKVVHRNLYKVH
                     QRVAATFRKGRVFLAGDSAHVNNSVGGLGLNGGIHDAMELVDTLHRVITKQGSEALLD
                     RYTRRRRTMNIEFVQEQTILNKKRLEERDPKTRQTRFDELRAIAEDTRKHKEFLLRTS
                     LIASVRKAQSLE"
     gene            complement(1972..2196)
                     /locus_tag="VF182_09640"
     CDS             complement(1972..2196)
                     /locus_tag="VF182_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437380.1"
                     /translation="MGIFYLDSKSRNFRVNAGQEAPIGSNAAPLFCATWIRQSKAARI
                     RVLELSTVGIAFDTGHTPAVEAGQFNAPTR"
     gene            2242..3423
                     /locus_tag="VF182_09645"
     CDS             2242..3423
                     /locus_tag="VF182_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437381.1"
                     /translation="MIAILSPYLCVVALQLLLLYPLHTTLDLPTWDEAIYLQQGGWFL
                     HGEGLGPISISPVYSLLYSVLIKIFGTVDSVFTMQYLVKVTVSGLLLLCLVEHLRSRL
                     LALLLTLIWVVSGINIFERVLVYHVALGFFLLALFSLNKHRGISLLLLVLCSLTRLEY
                     LFPTLAFAGYLIFISVPKFKRRGSQSTSVKRRVTAPIFMGFLLAAAILYVIVNVDGLN
                     PGTDRTWLAFNQNYALHEVEAGRYKLNPYLDYNFIIQEDFPGAESLADAFTISPGLFL
                     KHVARNIAMLPQAVLSFIIPYIGLRIWLNLWRVAGICDDNRDVRCGCQPTIHSVRLAR
                     RHRGAKRAALRNRCELAIASSNTSGLPAAASHIDHGSALPTVGWAHLLSGVKYHQLSA
                     V"
     gene            3392..3871
                     /locus_tag="VF182_09650"
     CDS             3392..3871
                     /locus_tag="VF182_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437382.1"
                     /translation="MLNIINSPLFSRWSLATLNALFILSIGVTSKPYVSKPPDRPVYD
                     KVMQLIELWPNEKVKLMAVGASWYAGYIGVDKVDAIEPLATASGDKIADQGTDLRLLL
                     EKYHPDAVLVNNELISSKNFKTASLEVLNSDGWVKQTIGADTVYFLGEKFKPARENK"
     gene            4068..4487
                     /locus_tag="VF182_09655"
     CDS             4068..4487
                     /locus_tag="VF182_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008865144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437383.1"
                     /translation="MPDETALPGAAPPQLVNVANLVYALHALSLVIGITTAATIVGAF
                     VFGVPSIIAVVINYFKREDARGTFLESHFRWQIRTFWFSLLWCIVAVMLFITILGIPF
                     ALIVLIAAGLWVIYRIARGWLALRDRKPMYTSGGLRI"
     gene            complement(4613..5350)
                     /locus_tag="VF182_09660"
     CDS             complement(4613..5350)
                     /locus_tag="VF182_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003574656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6437384.1"
                     /translation="MRYELYYQPSIQGRGEFIRLALEEAGADYIDVARNPNFGRPGIM
                     KFLEDSVLERPPFAPPFLKAGELLISQTANILQFLAPRLGLVPEKEDSRLWAHQLQLT
                     IADWLYETGQTHHPVANVLYYEEQKDEAKKRAAYFISTRIPKFLGYFERILKLNANGA
                     DFIFGEDVSYVDLSLFQMIAGLRYAFPKTMAQVEPQHPKAVLLHDRITARPRIAAYLA
                     SPRRLAFNEQGIFRHYPELEEAQTQNP"
     gene            6023..7081
                     /locus_tag="VF182_09665"
     CDS             6023..7081
                     /locus_tag="VF182_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008023106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6437385.1"
                     /translation="MQYKTFGRRTGLRVSELALGAANFGTRWGHGAERQDAKRVFDGY
                     VEAGGNFIDTADSYQFGESEELIGEFIAADRDDFVVATKYTLGTTATDGVSRTGNSRK
                     NMIRCLEASLKRLKTDRIDLYWAHFADGMTPMEEIIRAFDDLVHAGKIHYAGLSNFPA
                     WRISRADLLAEMRGWGPIAGIQVEYSLAQRTAERELLPMAEALGLGAALWSPLGGGFL
                     TGKYRTGDEGRINSFLARLVHTEKTQCERATLDTVMAIAKEVDRSPTEVAIAWILDKA
                     RLSTTALIPILGPRTYEQLAGNLRALEVSLSPEQIARLDETSQIALGTPHEQIHGSAA
                     AISGGKPELLRTRIIPVA"
     gene            7116..8138
                     /locus_tag="VF182_09670"
     CDS             7116..8138
                     /locus_tag="VF182_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018859046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEX6437386.1"
                     /translation="MAKMRAAQITGPNRPIEIVEREIPEPGHGEVRIRVEACGVCHSD
                     SFTKEGTLPGIQYPRVPGHEVAGIIDAIGPGIAGWTLGQRAGVGWHGGHCGYCDSCRR
                     GDFVTCQVAPQVTGIAYDGGYAEYMIAPAGALALIPEGLSAVDVGPLMCAGVTTFNPL
                     RNSGARSGDLVAVLGIGGLGHLGVQFAAKMGFQTVAIARGRDKEALARQLGARQYIDS
                     ESQNAAAELAKMGGAKIILATVTSGKAMSAVLGGLGVNGKLIILGAAHEPLEVNGPLM
                     IGGRRSIMGWPSGSSIDSQDTLSFSMLNDVRAMTEVFPLERAADAYDRMISGKARFRA
                     VLTMSR"
     gene            8349..9005
                     /locus_tag="VF182_09675"
     CDS             8349..9005
                     /locus_tag="VF182_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012408813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX6437387.1"
                     /translation="MKLISTNVGLPRIVTFNGDPVSTGIFKEPVAGRVMLRTLNLDGD
                     RQADLSVHGGRSKAVYLYPSEHYDYWKRELPGMNLPWGMFGENFTSAGLLESEINIGD
                     RFRVGSAVVIVTEPRMPCYKLGIKFGRSDIVKKFLASGRTGFYFGVWQEGEVGADDSI
                     ELIEKADLNVRVSDITRLYTREKHNLGLLRRAVQVEALPESWKSYFRQRIAKLSNERG
                     "
     gene            9109..9384
                     /locus_tag="VF182_09680"
     CDS             9109..9384
                     /locus_tag="VF182_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX6437388.1"
                     /translation="MAKSCEHLSGLKVEDFPPQRTPDACEECLTEGTFWVALRECRSC
                     GHVGCCDSSTGKHATKHFHDTQHPVMRAVPPASWIWCYVHQAQDVLD"
     gene            9638..11317
                     /locus_tag="VF182_09685"
     CDS             9638..11317
                     /locus_tag="VF182_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6437389.1"
                     /translation="MPERKLPVPLTQASGAERMFPTLTTPQIKRIAAHGRVRRIRSGE
                     VLIEPGERIVPFFVVLTGQLEIVRPSRAAEELVAVHGAGQFSGEVNMLSGRPALLRTR
                     ASEAGEVIELDREGLLALVQTDSDIGELIMRAFIIRRVELIAHGLGDVVVLGSNYCSG
                     TLRVKEFLTRNGHPYSYIDLDRDADVQDFLDRFHVSAADVPVVICAGEVVLRSPSNQQ
                     IADCLGFNDAIDQTQIRDVVVVGAGPAGLAAAVYAASEGLGVLVLETNAPGGQAGSSS
                     KIENYLGFPTGISGQALAGRAFTQAQKFGAQVVIARCAKRLVCEQKPYVIELDGGSQV
                     PARTIIIATGAAYQKPTLDNLSHFEGAGVYYGATFMEAQLCRGEEVVVVGGGNSAGQA
                     AVYLAETVKRVHMLVRSNGLAQSMSRYLIRRIEENPLINLRANTELTALEGGNHLERV
                     RWRNGQTAATETHEIRHVFIMAGAAPNTRWLDGCVGLDANGFIKTGADLLQEDLASAH
                     WPLARAPHLLETNLPGVFAVGDVRAGNVKRVASAVGEGSIAVSFVHRALHE"
     gene            11440..11598
                     /locus_tag="VF182_09690"
     CDS             11440..11598
                     /locus_tag="VF182_09690"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX6437390.1"
                     /translation="MCQECGKTHCCDDSPNRHASKHARTSNHPVIASAEPGERWLYCF
                     PDEAFAEY"
     gene            12010..12465
                     /locus_tag="VF182_09695"
     CDS             12010..12465
                     /locus_tag="VF182_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6437391.1"
                     /translation="MTNIFRHQKLALGAEALKMLHDKIPDPKKREESWENITQGIQSR
                     FFVDAVRRFDRLLADVEACLTEQSWMAGKEFSLADIGYAPYIRRLQDLQLQFLWDKRP
                     HLAAWFGRVAKRPGYQQGFEDWPNEAYASLMKEKGAEAQSRIQDILTLA"
     gene            12544..13125
                     /locus_tag="VF182_09700"
     CDS             12544..13125
                     /locus_tag="VF182_09700"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6437392.1"
                     /translation="MEAAALVIDALGRVRDIVRAALRDLAPEELLASPKPHIAWLVWH
                     LSRVQDANFSALMERPQLWIADGWHTRFNMAPSPRDYGSGHRQTPAQVEAFTITDKSL
                     LLDYLDAVFARTKAYLSTVTNDDLNRILNEPRYQPLPTLGIRLASVINCNTRHAGQIE
                     YLRGLIKHQGWFPVGESEFQASAAAQLHSSQKR"
     gene            complement(13257..14750)
                     /locus_tag="VF182_09705"
     CDS             complement(13257..14750)
                     /locus_tag="VF182_09705"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX6437393.1"
                     /translation="MEMLNFHLFVLTLALVGVVIIIAALLSGFIDRTDLPQVGIFLAL
                     GAMLGPAGLGLLDITLDSPILRVVATLSLVLVLFTDAVSLSIVQVRRHSALAFRVLGP
                     ATLLSAGLIALAAWLLLGFPLAAAAILGAALASTDPVMLRGLLRGQGLPQPVALALRL
                     ESGLNDAVLLPIVLIAMPFLGHDGLPTQTEWARIGLDLFLLGPGAGIAVGLLSIAALE
                     MIRRKTGVRRDYESIYSLGVAFAAFAAAEAVHGSGFLAAFAAGITIAALDVELCDCFL
                     EYGQTTAEMTLLFTFVLFGSSLIWTGITVLSWPVFLFAITVLLIRPIAFFISLAGTQL
                     DFRSRLLIAWFGPRGLSSLLLILVPVFAAVPGTEPLFSICCFIVLLSVVIHGGSVMAL
                     EHNESTVASLEKAPASGDNTIAANSSLTSPGNGSSKQDEIISIAEMREIQRSGAPHLA
                     LDVRAERNLADSDLRAGGSVRIPPELAVKRMMELDVPRQTWLFAFCA"
     gene            complement(15001..15303)
                     /locus_tag="VF182_09710"
     CDS             complement(15001..15303)
                     /locus_tag="VF182_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437394.1"
                     /translation="MEERKCLTLVDGKECGLDLLLVERELETQVEIYECALGHRTEHL
                     FAEVVKRTCTSFVDGKECGLPLALVQRELETATEVYECPLDHRTYVPLESETMDEQ"
     gene            complement(15481..17301)
                     /locus_tag="VF182_09715"
     CDS             complement(15481..17301)
                     /locus_tag="VF182_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6437395.1"
                     /translation="MISLPEKWRQLRQSFTHTPQAVGFVWRTNRWATVGLGLLTLGGA
                     LLPASQAWVGKLIVDGVVASIQSGQDAAQVRRVFVYLLLELGLFLLSAAFNHGRRLIQ
                     QLIQLQLANRIRAEIIAKALTLDLAYFEDSDFYDRLQNARREGGYKPVELINDTFLIV
                     QNSITLISFALLMLRFSPWLVVVLLATSIPSFISETRYAERGFRLLTWRAPETRQVNY
                     LSRLLTEDTAAKEIKLFNLGGSLLNRYTTLFDKFFEEDKSLALRRATAGFALGLIATL
                     GFYGSYAWIVWYTVQNKISLGDMTLYLAIFRQGQATFQSILSALGNIYENNLFMANFF
                     EFLCLEPRMKMTGHTRKLTVPLRGGIEFREIGFRYPDAEEWALRGVNLVIRPGEKIAL
                     VGHNGAGKTTLIKLLSRLYDPTEGSILIDGIDIRDLDPLELQQRIGVIFQDFVRYHLP
                     VRENIGFGQIDALEDLGRIAAAARKSGAESVVEELPQGYETMLGRWFHDGHELSLGQW
                     QKIALARAFMRDAEILVLDEPTASVDARTEYEIFQNFKTLTEGKMAILISHRFSTVRM
                     ADRIAVIQEGRIAELGTHDELLRREGIYAELFSMQAEGYR"
     gene            complement(17589..18587)
                     /locus_tag="VF182_09720"
     CDS             complement(17589..18587)
                     /locus_tag="VF182_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437396.1"
                     /translation="MAAFLRTSAVAIALLTAAVLVCASHSQAQPGLRKVRVSIPAANV
                     TYLPFYAAKDRGYYKDEGIDVEFILMPAALASTAALTGDIDYNGAVTGVVAAAVRSQP
                     IKAVIFTMRSPVQGLMAKAEIKNFQQLKFKKIAVSSPGATTDLATRYTLRREGLEFGR
                     DYSTVYVASEAGRLAALDAGIVDAAMLSVPENILARQKGFTELAFSGDYIEFPQNGFG
                     ASIKKIKDNPDEIYRMVRGTLRGLMFVSDKNNKDACLDMIAKHWSIKNRAMASEMYDY
                     MVRVMLPDGAINMNGLQALVDQQRESAKVSEPISAAQVIDYSFVEKARKELAAGRQ"
     gene            18968..19945
                     /locus_tag="VF182_09725"
     CDS             18968..19945
                     /locus_tag="VF182_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437397.1"
                     /translation="MTRYLAVIYSFIWLLVLSLGDGRGAERARGQPHTIRIAYVSPVT
                     TMAPIWIASEIGAYQREGLDPRIVFIDARVSIAALVAGEVDAVVISAPSVIPAVLSGA
                     NIAFIAGLHNKMIFSFHAQPDIQSSAQLRGKIVGTNRPGTPADYGNRVALTKMGLKPT
                     DVQLMPLGSSGVMWSALQSRQVAGVTLAPPYSFRADAMKFSRLVDTYDKPYQNTGVVV
                     RKERIHSSADTWLRLLRALRQANLRWYEDPKLAMFVLSKYTQQSDPDVLQKTYDFESN
                     PPGFTKDLKVSDTGLQGILDFLASTVRPEAGKASPKEFYDTTILDRLEK"
BASE COUNT         4492 a         5302 c         5688 g         4477 t
ORIGIN      
        1 cgatgtgttg cgcatgatgg agcgaggcgg gttgatttcg atgatcggtt cggagaattt
       61 cttttggagc tcggatcaag cgattgtcgc gctggaaaac cgaagctgcc gttattgcgc
      121 caacgagaag cctgctatca tcaagcaaca agtcgttgcg atctgatcgg ttatgtgagc
      181 gacaactgct agtctcccgc ggcggattac gcaagtagat tttgccgtgc gaaccgctcc
      241 gtcggcccgt taaccaagaa gcgatcgctc cggttgatgc ctaccatgag tctcgatgat
      301 atgatatccg atcgatgatc ggctgatcca gagaatccct tacaatgaaa aaccctcgcg
      361 tcatcatagc cggcggtggt cccgtcggcg ttgtgtcagc gctggcctgc gcacaacatg
      421 gctacccggt aacgctcctc gaagccgagg ataagatcga cgacaatccg cgagcggcaa
      481 caactcaccc ctcgactctc gaactgatcg cccgggtcgg cctgatcgat caattcattc
      541 gagaggggtt ggtggcgcgg ttctttcaat tctgggataa gccgtcgcgc actaaaatcg
      601 tcgaattcga tcatgacatc ctgcgtgacg agacgcccta tccatttgtc gttcaaaccg
      661 agcaacacaa gcttgcccgg atgggtatcg aacggctcag gacttttcct gatgccgaag
      721 tgcgcttttc tagccgcgcc accacggttg atcaagatgc cgacggcgca accgtgacca
      781 tcgagggttc tcagggcaca gaaaccttac gcggtgaata tgttatcggc gcagacggtg
      841 gccgcagcac cattcggaag gctctggaca tcgaatttga ggggtttacc tggcccgagc
      901 gctttctcgt gctgaccacg atcgaggact ttcaaaaatt gttgacgggc tgctgctatc
      961 gtaattatct ggccgacccg gacgagtgga ccaacctatt caaggtggcg ggcgatgacg
     1021 gcaagggccg ttggcgcgcg gtttttccga cacgagtgga agagagcgat gaacaggctc
     1081 tgggggatga ctccacctat tcgcggctgc aacgcgtatt tcccatggag cgaccctata
     1141 aagtcgtgca tcgcaattta tacaaggttc accaacgcgt tgcggcgacg tttcgcaagg
     1201 ggcgggtgtt cctcgccggc gattccgccc acgtcaataa ctctgtcggc ggcctgggtc
     1261 tcaacggcgg gatccatgac gctatggagc tggtcgacac tctccaccgg gtgatcacca
     1321 agcagggaag tgaggcgttg ctcgatcgct acacccggag aagacggacg atgaatatcg
     1381 aattcgttca agagcaaacg atactcaaca aaaagcgact ggaggagcgc gaccccaaga
     1441 cgcggcagac gcgcttcgat gagttgcggg caattgccga agatacccgc aagcataagg
     1501 aatttttgct acgcacttca ctgatcgcca gcgtgcgcaa agcccagagc ttggaatagt
     1561 cttacggaaa gtcgactaga tagccggtac actcgcctat ctcaccagcg tcgagcgcct
     1621 ttgtcacggg aaagtctaaa aacttctcgt cggtgcgcgt tcgagcgcag aacgtgggca
     1681 gattgcctcg cgctaacggc cttacaattc ccatcgactg aaaatgaccg actagcgagt
     1741 gatcgttaag gtgattgctc tcgtgccctc caaccgacac aagcggttat tagccgctcc
     1801 cgcgtgttgg attgtccagt catatgcaat atgaaagatg gaatgctttt gtaccgtcga
     1861 gtttagcaga gcataacagc ggagccatgg cgatgagccc attctaaagg aatgaaactg
     1921 atccagcgta actcattgaa tcggtaaatc cgaagaacct gttcatgctc atcatcgtgt
     1981 cggcgcgttg aactgccctg cctcgaccgc cggagtatgt ccggtatcga aggcgatgcc
     2041 aacggtgctc agttcaagga cgcggattcg tgccgccttc gattgacgta tccatgttgc
     2101 acaaaacaac ggcgcagcgt tcgagccgat cggcgcctcc tgaccagcat tgacccggaa
     2161 attgcgcgac ttggagtcga ggtaaaagat ccccaatcgc aatttcaccg taagattgtg
     2221 aaccgctccg gaggatggaa gatgatagct atactttccc cgtacctgtg tgtggttgcg
     2281 cttcagttat tgctgcttta tccgctgcat acgaccttgg acttgccgac gtgggacgag
     2341 gctatttacc ttcagcaggg tggctggttc cttcatggcg aggggctcgg acctatatcg
     2401 atctcgccag tttacagcct gctttattcg gtattaatca aaatattcgg caccgttgat
     2461 agcgttttca ccatgcagta cctggtaaag gtcactgtct ccggattgct tcttctttgc
     2521 ctggtagaac atcttcgctc gcgcctgcta gccttactgc ttacgttgat ttgggttgtc
     2581 tcgggaatca atattttcga aagagttctg gtatatcacg ttgccttggg ctttttttta
     2641 ctcgcgttat tttcattgaa caagcaccgc ggaatttctt tgctgctgct tgttttatgc
     2701 tctctgacga gattggagta cctgttcccg acgctcgctt ttgccggtta tctgatcttc
     2761 atctccgtcc ctaaattcaa gcgccgcggg tcacaatcta cttccgtaaa gcgtcgtgtc
     2821 accgcgccga ttttcatggg attcctgctt gcggcggcga tcttgtacgt catagtcaat
     2881 gttgatggcc ttaatccggg caccgaccgg acctggttag cgttcaatca gaattatgcc
     2941 ctccatgagg tagaagccgg acgctacaaa cttaatccgt atcttgatta caacttcatc
     3001 atacaagagg actttcctgg tgctgaatcg ttagccgatg ctttcacgat tagtcccggg
     3061 ttatttctaa agcatgttgc cagaaatatc gcgatgcttc cgcaagccgt cctgtcgttc
     3121 attatcccgt acattggctt aagaatatgg cttaatctat ggcgtgttgc tgggatttgc
     3181 gatgacaatc gtgacgtacg ctgtggttgt caaccgacga ttcattctgt caggctggct
     3241 cggcgccatc gcggagcgaa gagagctgct ctacgtaacc gttgcgagct tgctatcgct
     3301 agctccaata cttctggttt acccgcggcc gcatcacaca ttgatcatgg ttccgctttg
     3361 cctactgtgg gctgggctca cctgttgtca ggtgttaaat atcatcaact ctccgctgtt
     3421 tagtcgatgg agcctggcta cgctcaatgc gttgttcatc ttgtctattg gggttacctc
     3481 caaaccctat gtctccaagc cgcctgaccg ccctgtttat gacaaggtaa tgcagctgat
     3541 cgagctatgg ccgaatgaga aggtaaagct tatggcagtg ggggcttcct ggtatgcagg
     3601 ctatataggc gtcgacaagg ttgacgcgat tgaaccgtta gcaactgcat caggcgataa
     3661 aatagcagat caaggcactg acttgcggct tttgctggaa aaatatcacc cggatgctgt
     3721 gctggttaat aacgagctga ttagttcaaa aaatttcaag accgcttccc tcgaagtttt
     3781 gaactctgat ggatgggtta agcagacaat cggtgccgat accgtctatt ttttagggga
     3841 aaaattcaag ccggcccggg agaacaaatg agcggcgtta tgagcggaag cgatcaggcg
     3901 aggaggttgt ctggatgatt gcggaacagg ggaggcagat ggattccagg cacgcgccgc
     3961 gaacccagtt gcatgcgtgc ttgcgatgat tttgcttgac ctttacccat cacctgataa
     4021 ttgaaacgag tttggaatat ctgtgatcga tcaaggcaga aattctcatg cccgatgaaa
     4081 ctgcactccc tggagccgcg ccgccgcagc tcgtcaatgt tgctaatctg gtgtatgcgt
     4141 tgcatgccct gagcctcgtc atcggtataa cgaccgcagc gacgatcgtc ggcgccttcg
     4201 tctttggtgt gccgtcgatc attgcagtcg tgatcaacta cttcaaaaga gaagacgcaa
     4261 gaggaacctt tctcgagtct cacttccgct ggcagatccg aacattttgg ttttcgcttc
     4321 tctggtgcat cgtcgccgtg atgctgttca ttacaatcct gggcattccc tttgccctaa
     4381 tcgtcttgat cgcggccggc ttatgggtca tctatcggat cgctcgcggc tggctggcgc
     4441 tacgcgatcg aaagccgatg tataccagcg gtggattgcg aatctgaccg gcaaaagagc
     4501 cgttaaattc aaggggtgga catgacgctc agctcgttct gaaaaaattg aagcgaagtg
     4561 agccgatgac gcggcgaatc ggctgcttgc ggcgtttagg gcagtctttt attcatggat
     4621 tttgcgtctg cgcctcttcc aactccggat aatggcggaa gatgccttgc tcgttgaacg
     4681 cgaggcggcg cggtgacgct aagtacgcgg cgatccgcgg acgcgccgtg atgcgatcgt
     4741 gcagaagtac ggccttcggg tgttgcggtt cgacttgtgc cattgtcttt ggaaaagcgt
     4801 agcgcagtcc cgcgatcatc tgaaagagcg acagatcgac atagctgacg tcctcgccga
     4861 aaataaagtc ggcgccgttg gcattgagtt ttagaattct ttcgaaatag ccaaggaatt
     4921 tgggtatgcg ggtcgagatg aaatatgcgg cgcgtttttt cgcctcgtcc ttctgctcct
     4981 cgtaatacag gacgttggcg accgggtggt gcgtctggcc ggtttcatag agccaatcgg
     5041 ctattgtcag ttgcagctga tgcgcccaga ggcggctgtc ctctttttcg ggtacgagac
     5101 ccagacgggg ggcaaggaac tggagaatgt tggcggtttg cgaaatcagc agctcgccag
     5161 ctttcaggaa cggcggggcg aacggcggac gttccaaaac cgaatcctcg agaaacttca
     5221 taattcccgg acgaccgaaa tttggattgc gcgcgacgtc gatataatcc gcgcccgctt
     5281 cttccagcgc aagccgaata aactcgccgc ggccttggat cgatggttga tagtagagct
     5341 cataacgcat ggtaatcctc ctctcttaat caaaatattc agtggcccag cattagatcc
     5401 agatgaaaaa atgtgtcgag tagcagcatt tccaaacggc caaactcgcc ttcggctagc
     5461 ttgcccggtc ttaacactaa tcgaagaata atacacgctg agtcagattt gaacctgaga
     5521 gcgctcccaa tttggtgatg gaagaatgtg ggtcagtctt tcggttgcgg tttatagcta
     5581 atcgtctact cttaagcctc aatcagttct taatcgagta tcttattcgg tgcgactaaa
     5641 catcgttgcc gttctgcttt agtgctgaag cagcaacggg cgaacctctc cgaagtttcg
     5701 ccggctgagc ctatgcgttt tacaagcttc acaatggtct tcattgcatt cgcaactctg
     5761 tgggaagtca gaaacccgag atcatgattg caaaagttcc gatgtgcaac agaggccggt
     5821 gcgcctcatc ggtgactccg ttgaagctct tgaggctgat cggcattgca ttcctgatcg
     5881 gttcaagcgg accgagtgca agagcttatt ggaagccgac gtcaatcgag ggatcggtca
     5941 gtgcgttgtc taagctaacg ctagagtgaa attacttgca gtccggacgc gagaattcac
     6001 atgtgagctg aggagtgcaa tgatgcaata taaaactttt gggcggcgca cggggttgcg
     6061 ggtttctgaa ctagctcttg gcgccgcaaa ctttgggacg cgctggggtc acggcgcaga
     6121 gcgccaggat gcaaaaaggg tcttcgacgg ttacgtcgaa gcggggggca acttcatcga
     6181 caccgccgac agttatcagt ttggcgagtc agaagagctg atcggagaat tcatcgcggc
     6241 ggatcgagac gactttgttg tcgccactaa atacacgctg ggaacgactg ctaccgacgg
     6301 cgtctcgcgt acgggcaata gccgtaagaa tatgattcgc tgcctggagg cgagtctcaa
     6361 gcgactgaag accgaccgca tcgatctcta ttgggcgcat ttcgccgacg gcatgacgcc
     6421 gatggaagaa attattcgtg cgtttgatga tctagtgcac gccggcaaaa ttcattatgc
     6481 ggggttgtcc aactttcccg cctggcggat ttctcgcgcc gatctcctgg cggagatgcg
     6541 cggctggggg cctatcgccg gcatccaggt cgaatatagc ctggcgcagc gcaccgcaga
     6601 acgggaactt ctgccgatgg cggaggcgct cgggttggga gccgcgctat ggtcgccgct
     6661 cggcggcgga tttctcaccg gcaagtatcg cacaggcgac gagggacgga tcaattcgtt
     6721 cctcgcccgg ctggtccaca ccgagaagac gcaatgtgag agggcgactc tcgatacagt
     6781 aatggcaatc gccaaggaag ttgaccgatc gccgacggaa gttgccattg cctggatact
     6841 ggataaggcg cgcctgtcca cgacggcatt gattccgatc ctcggcccgc gcacttacga
     6901 acaactcgcc ggcaatctca gggcgctgga ggtaagtcta tcgccagagc agatcgcccg
     6961 gctcgatgag acgagtcaga tcgcgttagg cacgccgcac gagcaaatcc atggctctgc
     7021 ggctgccatt tccggcggca agccggagtt gcttcgaacg aggatcatac ccgtggcgta
     7081 aagatcgaga cgaaaagcaa acgatcggag gcaatatggc aaagatgcgc gccgcacaga
     7141 ttactggccc gaaccggccc attgaaatcg ttgagcgtga aatacccgaa ccgggccatg
     7201 gagaggtgcg tatcagggtg gaagcttgcg gcgtctgcca cagcgattcg ttcaccaagg
     7261 agggaacctt gcccggcatc cagtacccgc gggtccccgg gcatgaggtt gcaggaatca
     7321 ttgatgcaat cggtcccggc attgccggat ggacactggg gcagcgtgcc ggtgttggct
     7381 ggcacggcgg acactgtggc tactgcgact cttgccggcg tggcgatttc gtcacctgtc
     7441 aagtcgctcc ccaagtgacc ggcatcgcct acgacggcgg ctatgccgaa tatatgatcg
     7501 cacccgcggg cgcattggcg ctgattccag aagggctctc tgcggtggat gtaggaccac
     7561 tgatgtgcgc cggggtcacc accttcaatc ctcttcgcaa cagcggcgcg cgctccgggg
     7621 atttggtggc ggtactcggg atcggcgggc ttggtcattt gggagtccag ttcgcggcga
     7681 agatgggttt ccaaacggtt gccatcgctc gaggccggga caaggaagcg ctcgctcgac
     7741 aacttggcgc gcgtcagtac atcgatagcg agtcgcagaa tgctgcggcg gagttggcca
     7801 agatgggcgg ggctaaaatc attctcgcaa cggtgacaag cggcaaagca atgagcgcgg
     7861 tcctgggcgg tctcggcgtc aatggcaagc taatcatcct cggcgctgcg catgagccgc
     7921 tcgaggtgaa tggacctctg atgatcggcg gacgacggtc aatcatggga tggccctccg
     7981 gcagctctat cgattcgcag gatacgctgt cgttcagcat gcttaatgat gtgcgcgcta
     8041 tgactgaagt ttttccgctc gaacgcgcgg ccgacgccta tgaccgcatg atcagcggca
     8101 aggcgcgctt tcgcgcggtt ctaacaatga gtcgttaagc ctgaaatact tagacgataa
     8161 cgatggtcgc gcaatgaagc tttgcgccgc aaagcaatgg acgctctcga gtttaacgct
     8221 gttcaattcg ggtcggtaaa ttcggtcaaa gctctgttga atctgaacta tcgttggacg
     8281 cgggtcggat taaaggatcc atacggggcc gtttcgccat cgagagaagg tgtgtgatga
     8341 aaactgacat gaagctgatc tccaccaacg tcggactgcc gcggatcgtc acattcaatg
     8401 gcgatccagt ctcgacagga attttcaaag agccggtcgc agggcgggta atgctgcgaa
     8461 ccctgaacct cgacggtgac cgtcaagccg atctttccgt tcacggcggc cggagcaaag
     8521 cggtctacct ctatccctct gaacactacg actattggaa gcgcgaattg cccggaatga
     8581 atctaccctg gggcatgttc ggtgaaaatt tcaccagcgc gggacttttg gaatccgaga
     8641 taaacatcgg tgacaggttt cgcgtaggtt ccgccgtcgt aatagtgacc gagccgcgga
     8701 tgccatgtta caagctcggc atcaagttcg gtcgctccga catcgtcaaa aaattcctgg
     8761 caagcgggcg caccggcttc tatttcgggg tttggcaaga aggcgaggtt ggcgcggatg
     8821 actcgatcga acttatagag aaagccgatc tcaatgttcg ggtcagcgac atcacccgac
     8881 tctatacccg cgagaagcat aaccttggac tgcttcgccg cgccgtacaa gtcgaggctc
     8941 tgcccgaaag ttggaagagc tattttcgcc agcgaattgc taaactaagt aatgagcgcg
     9001 gctagcgcgc cattcttcgg agagtgccaa ccgcggctga gatgaatatc tcacggggaa
     9061 gaatcgaccg gccgcaaaaa aaatttgacg ctgagatcgg aggagcctat ggccaaatcg
     9121 tgtgaacatt tatcgggcct gaaagtcgag gattttccgc cgcagagaac tcccgatgcc
     9181 tgcgaggaat gcctgaccga ggggacattt tgggtcgctc ttcgcgagtg ccgatcgtgc
     9241 ggtcacgtcg gctgttgcga ttcttcgacc ggcaagcatg ccaccaaaca tttccacgac
     9301 acgcaacatc cggtgatgcg cgctgtgccg ccagcgtcct ggatctggtg ctatgtgcac
     9361 caggcgcagg atgtgctcga ttaggctgcc gccgctccgg gtgccaattg gcgatcgtaa
     9421 tcctcgaccg gctagcacgc ccgacccaaa cgtttgggat gagcgaacag gggtcatgag
     9481 tgctgttcgc atgaacactc ctgggttgca tctctacgca tgagcctcac tggttgaagc
     9541 gatctagcgt gaccttgaaa gattcggatg taaagtccca acgttcaatt tgagctgcct
     9601 tccgacacaa cagaaagtgt atcaaggagt attcttaatg cccgagagaa agctgccagt
     9661 gcccttgact caggcttcgg gtgcggaacg gatgtttccg acactgacga cgccgcagat
     9721 taaacggatc gccgctcatg gacgcgtacg tcggatccgg agtggagagg tccttatcga
     9781 gccaggagag cggattgtgc cgttcttcgt cgtcctgaca ggtcagctcg agattgttcg
     9841 gccgtcgcgc gccgccgagg agctcgtcgc tgttcatgga gccggtcagt tcagcggtga
     9901 ggtcaacatg ctttccggcc gcccggcgct gctccgcacg cgggccagcg aggcaggcga
     9961 agtgatcgag ctagatcgtg agggcttgtt ggcgctggtc cagaccgaca gcgacatcgg
    10021 tgagctcatc atgcgcgcct tcatcattcg tcgtgtcgag ctgattgcgc acgggctggg
    10081 agatgtcgtg gtcctcggtt caaattattg ttcgggaaca ctccgcgtca aagagtttct
    10141 gacgcgcaac ggccatccgt actcctacat cgatctcgac cgtgatgccg acgttcagga
    10201 ttttctcgat cggtttcacg tgagcgccgc agacgtgccg gtggtgatct gcgccggtga
    10261 ggtggtgctt cgaagcccga gcaatcagca gattgccgat tgtctcggtt ttaacgatgc
    10321 catcgatcag actcagattc gtgacgtggt cgtagtcggg gccggccccg ccggacttgc
    10381 ggcggcggtg tacgcggctt cggaaggcct cggtgttttg gttttagaga cgaatgcgcc
    10441 aggcggacaa gcgggatcaa gctcgaagat tgagaattac cttggatttc cgaccggcat
    10501 ttccggtcag gctctcgccg gacgagcttt cactcaggcg caaaaattcg gtgcgcaggt
    10561 agtcattgcc cggtgcgcca aacgcctggt ttgcgagcag aaaccatatg tcattgagct
    10621 tgacggcggt tcgcaggtgc cggcgcgcac cataattatc gcaaccggtg ccgcgtacca
    10681 aaaacccacc ctcgataatc tgtcgcattt tgaaggggcc ggtgtgtact acggcgcgac
    10741 gtttatggaa gcccagttgt gccgcggtga agaagtggtg gtagtgggcg gcggaaattc
    10801 cgccggccag gctgcggttt atcttgccga gacggtcaag cgtgtgcaca tgctggtccg
    10861 atcgaatggc ctagcgcaga gcatgtcccg ttatctaatt cgccgcatcg aggaaaatcc
    10921 gctgatcaac ttgcgagcga acacggaact gacggctctc gaaggcggta accatctcga
    10981 acgagtacgc tggcgcaatg gccagaccgc agctacggag actcacgaaa ttcgccacgt
    11041 ctttatcatg gcgggcgctg ctcccaatac tcgttggctc gatggttgtg tcggactcga
    11101 tgccaatggc ttcatcaaga cgggagcgga tctgttgcag gaagatttgg catcggcgca
    11161 ttggccgctc gccagggctc ctcatctcct cgaaaccaat ctacccggcg tgttcgcggt
    11221 cggtgacgtg cgggccggca atgtcaaacg agtagcatcg gccgttggcg aaggatcgat
    11281 tgcggtctcc ttcgtccatc gagcgctgca cgagtaagag gaaaaacgat ggcagaagga
    11341 acgtgtagtc atatcgaggc gattacgact gtgaaacgcg cgaaacgtcg ggagtgcgaa
    11401 gaatgcgtga aaaccggctg accatgggta catctcagga tatgtcagga atgcggcaag
    11461 acgcactgtt gcgatgattc accgaaccgc cacgcctcca agcacgcgcg aacgagcaat
    11521 catccggtga ttgcttcggc tgaaccaggg gagcgttggt tgtactgctt tccggatgag
    11581 gcgtttgccg agtactaatc gatatcgaag gaacaccaac agtcactaag aaacctttcg
    11641 aattcgctct gcagacttaa gtaaatcata ggaggaaaag tgatacctcg cggcgaggca
    11701 gtaaagagtt tcctccaaat tcaaccagct cttcagggat gcttgcgggc cggcgtagaa
    11761 tgctgacggt ttcgaaatat tgctcggcgc ctggtcactc gacggctttc ttgccgcgca
    11821 gttctagttg ctagagaacg caccgaaccc cttgattgcg aatcgaacag gatttgcggg
    11881 gttcccagag cctggctttt ttgctccctc tgcgcctacg caactcggcc agcactatct
    11941 tcatcgagga ttcagccggc gcgtgaccgg gcaaaaaatc gtgactctca tcgcacaagc
    12001 agccaagcga tgacgaatat cttccggcat caaaaactcg ctcttggcgc ggaggcgttg
    12061 aagatgcttc atgacaagat ccccgatccg aaaaaacgcg aggagagctg ggaaaatatt
    12121 acacaaggca tccagtcgcg tttctttgtc gatgccgtac ggcgcttcga caggctcctg
    12181 gccgacgtcg aagcttgctt gacagaacaa tcctggatgg caggaaaaga attctcactg
    12241 gccgatatcg gttatgctcc ctacatccgt cgtttgcagg atcttcaatt gcagttttta
    12301 tgggacaaac ggccgcatct tgccgcatgg ttcggcaggg tcgctaagcg accgggttat
    12361 cagcagggct tcgaggactg gcccaatgaa gcttacgcct cgctcatgaa agagaagggc
    12421 gctgaagcac agtcgcgaat tcaagatatc ctaacgctgg cgtaaacaat cggcttcaac
    12481 cactcaacgc tggaacgacg ggaatccgat cgcgaaaact aacgctgttg aacggaggct
    12541 cgaatggaag ctgcggcgct ggtaatagac gctctggggc gagtgcgcga tatcgtgcgg
    12601 gcagccttga gggatttagc tcccgaagaa ctgcttgcct cacccaaacc tcatattgcc
    12661 tggctggttt ggcatttgtc tcgggtccag gacgccaact tctccgcgct gatggagcgg
    12721 ccgcaattgt ggattgccga cggatggcat actcggttta acatggcgcc tagtccgaga
    12781 gactacggct cgggtcatag acaaactccg gcacaggtag aagcgttcac cataaccgat
    12841 aaatcgctac tgctcgatta cctcgacgct gttttcgcca ggaccaaggc ttatctatcg
    12901 acagtcacga atgacgattt gaatcgaatt ctgaacgagc ctcggtatca accgttgccg
    12961 acgttaggta tccggctcgc cagtgtgatc aactgcaaca cgcgccacgc gggtcaaatc
    13021 gaatatttac ggggcttgat caagcaccag ggttggtttc cagtcggcga gagtgaattc
    13081 caagccagtg cagcggcgca gcttcattcg tcacagaagc gttagtcgag cgctttcgat
    13141 tcgaccggta atccggcgga ttgccacgct tgccatccac ccaccagcgc gcgcgctttg
    13201 ctccaacccg cggtctgtag ctctcgcgcc acacggacgc ttgttgcttc atccggtcaa
    13261 gcacaaaatg cgaacagcca cgtctgccgc ggcacatcca gctccatcat acgcttgacg
    13321 gcgagttcgg gtggaattct gactgacccg ccagctcgaa gatcgctgtc cgcgagattt
    13381 cgctctgccc gaacatccag agccaggtga ggcgcccccg atctctgaat ttcgcgcatc
    13441 tccgcgatcg agataatctc gtcctgttta ctgctcccgt tgccgggtga agttagcgac
    13501 gagtttgctg cgatggtatt gtcgcctgac gccggggcct tctccaacga tgccaccgtg
    13561 gattcattat gctcgagcgc catcactgag ccgccatgaa tcacgaccga aagtaagacg
    13621 ataaagcaac agatagagaa caaaggctcg gtgccgggaa cggctgcgaa gaccggcacg
    13681 agaatcaaca acagcgagct caggccccgt ggtccaaacc acgcgatcaa caagcggcta
    13741 cggaaatcga gttgggttcc agccagggaa atgaagaacg cgattggccg gattaacagt
    13801 acggttatcg cgaacaggaa gaccggccaa ctcagcacgg ttattccggt ccagattaaa
    13861 gaactgccga atagaacgaa ggtaaagagg agcgtcattt cagcggtggt ttggccgtat
    13921 tcgagaaaac agtcgcaaag ctctacgtcc agcgcggcaa tcgtgatccc ggcagcaaac
    13981 gcggctagga agccgctgcc gtgaacggcc tcagccgcgg caaaagccgc gaaggccacg
    14041 cccaaagagt agatcgattc atagtcgcgc cgtactccgg tcttgcgccg gatcatctcg
    14101 agggcggcga tcgagagcag cccgaccgcg attccagcgc caggtcctag aagaaaaaga
    14161 tcgagtccga tgcgcgccca ctcggtctgg gtcggcaatc cgtcatggcc gagaaatggc
    14221 atcgcgatca agacgatggg taacagcacg gcgtcgttga gaccgctttc cagccgtagc
    14281 gccagcgcca cgggttgagg aagcccctgc cctcgaagca ggccgcgcaa cattaccgga
    14341 tctgtcgaag cgagagccgc gccaaggata gctgcggccg ctaaaggaaa tccgagcaac
    14401 agccacgccg cgagagcgat aagtccggct gagaggagtg ttgcgggtcc caggacgcgg
    14461 aatgccagcg cgctatggcg ccgcacttgt acgatactga gactgacggc atccgtgaat
    14521 agcaccagaa ccaagctcag tgtggctacc acccgaagaa tcggcgagtc cagagtaatg
    14581 tccagcaacc ctaatcccgc cgggccgagc atcgcgccga gcgccaggaa gatcccgact
    14641 tggggtaggt cggtacgatc gataaatccg gagagtagcg cggcgatgat gatgaccacg
    14701 ccaactaatg cgagggtcag cacgaaaaga tgaaagttga gcatttccat ggaagttctc
    14761 tggatgtttg tggtcgagga tgtttctctc agcgttctga agggaaaata aaatgccgcc
    14821 tcaagatacc agttcgcggt ctcggtcgac aactgactta ggcgagctta ggcgaaagag
    14881 tcgccaggtt ttctaggtag ttttattcta ttggagctcg gcaaccagaa ctgtgatgcg
    14941 ttcgtgggaa gaagggcgtt ggagccgaaa agtcaacgaa caaatggcat gtggcggcgg
    15001 tcactgctca tccatggttt ccgattcgag tggcacatag gtgcggtgat ccagcgggca
    15061 ttcgtaaact tctgtggcgg tttcgagttc tcgctgtacc aaagcaagtg gcagcccgca
    15121 ttccttaccg tcgacaaagg aggtgcacgt tcttttgacg acttcagcaa acaggtgctc
    15181 tgtgcgatga cccagcgcgc attcgtaaat ttctacttgg gtttcaagct cgcgctcgac
    15241 caggagtagg tcaagaccgc attctttacc atcgaccagc gtgagacatt ttctttcttc
    15301 catctctgtt tcctcctcgt gatcgcaatc gattcatcat cgccctgcta tttccacatt
    15361 tgcccgctac cgttcggggt gtcaagtagg acgaaacaat ctcgagcgac aaataaacgg
    15421 gtggttccaa gtcgaattac ggggttgaag tctaggcaat cctggatcta aagacgacac
    15481 tcagcgatac ccttcggctt gcatgctgaa gagctcagca taaatgcctt cgcggcgcag
    15541 aagttcgtcg tgggtgccga gttcggcaat gcggccttct tggatcacgg cgatgcggtc
    15601 ggccatgcgc accgtggaaa aacgatgcga aatgaggatt gccattttcc cttcggtgag
    15661 tgttttgaag ttctggaaaa tttcgtattc ggtgcgcgca tcgaccgatg cagtgggttc
    15721 atcgagcacc aaaatctcgg catcgcgcat aaaagcgcga gccagggcga ttttttgcca
    15781 ctgtccgagg gagagctcgt gaccgtcgtg aaaccagcgc ccgagcatgg tttcatagcc
    15841 ctgcggaagt tcttcgacca ctgactccgc gccacttttt cgggcagccg cagcaatgcg
    15901 tccgaggtct tccagcgcat cgatctggcc aaatccgatg ttttctctca cgggcaggtg
    15961 atagcgcacg aagtcttgaa agatcacgcc gattctttgc tgcagttcta acgggtccag
    16021 atcacggata tcgatgccgt cgatcaaaat gctgccttcg gttggatcgt aaagccggct
    16081 caagagtttt ataagcgtgg ttttgccggc tccattgtgg cctaccagcg caatcttttc
    16141 gcccggccgg atgacgagat tgacgcctcg cagggcccat tcctcagcgt cgggatagcg
    16201 gaagccgatt tcgcgaaact cgatgccgcc gcgcagcgga accgttagct tgcgtgtgtg
    16261 tccggtcatt ttcatgcgcg gctcaaggca aagaaattcg aaaaagtttg ccatgaataa
    16321 gttattctca tagatattgc ccagggccga gagaatcgat tgaaacgtcg cttgcccctg
    16381 gcggaaaatc gccaggtaaa gcgtcatgtc gccgagggat attttattct gcacggtata
    16441 ccagacgatc cacgcatagg agccgtaaaa tcccaacgtg gcgatgagtc ctagggcgaa
    16501 tcccgcagtg gcgcggcgta gggccaggga tttgtcttcc tcaaagaact tgtcgaacag
    16561 tgtcgtgtaa cgattaagca agctgccgcc tagattgaac agcttgattt ctttagctgc
    16621 ggtgtcttcg gtcagcaggc gcgagagata attgacctgg cgggtttcgg gcgcgcgcca
    16681 ggtgagcaag cgaaagccgc gctccgcata gcgggtttcg gagatgaacg acggaatact
    16741 ggtcgcgaga aggactacca ccagccacgg gctaaaacgg agcatcagga gagcaaagga
    16801 gattaaggta atcgagttct gcacgatcag aaaagtatcg ttgatcagtt ccaccggctt
    16861 gtacccgccc tcgcgccggg cattttgcag ccggtcgtaa aaatcagaat cctcgaaata
    16921 agcgagatcc aaagtcaggg ctttggcgat gatctcggcg cgaatgcggt tggcaagctg
    16981 gagctggatg agttgttgaa tcagacgccg gccatgattg aacgctgcgc tgaggaggaa
    17041 aagaccgagc tcaagcagca aatagacaaa tactcttctc acctgcgcgg cgtcctggcc
    17101 gctttggatg gacgcgacta cgccgtcgac gatgagcttt cccacccagg cttggctcgc
    17161 cggcaataaa gcgccaccca aggtcaacaa acccaaaccc acagttgccc aacggttggt
    17221 gcgccagaca aagccaacgg cttggggcgt gtgagtgaac gattggcgca gttggcgcca
    17281 cttttctggc agagaaatca tccggcaggt ttaagattac agtttcgggt ctctgattga
    17341 aactataaac ccgcgctgca aagtcggagc tcaggtaggg cgagcgttat gcgtcctgag
    17401 ttgctatcca ggatcggaaa cggatcgaat tcgtcgcgca atttatggcc gaacgcgaga
    17461 cggttccgga ggctggcaag aaggataccg gaatcgatgt gggcgactct ctagctttga
    17521 aatgattctt ctttgatggc gtagcagcgt agggagatca attcgaacga catggtccag
    17581 ggatcaaatt attgtcgtcc ggcagctaat tccttgcgcg ctttttccac gaagctgtag
    17641 tcgatcacct gagccgcact gatgggctct gatactttgg cgctttctcg ttgttgatct
    17701 accagcgcct gaagtccgtt catgtttatg gcgccatcag gcagcatcac cctgaccatg
    17761 taatcataca tttcgctcgc catggcgcgg ttctttatgc tccagtgctt ggcgatcatg
    17821 tcgaggcaag cgtccttatt gttcttatct gagacaaaca tcagtccacg cagggtgccg
    17881 cgcaccatcc ggtaaatctc gtcaggattg tctttgatct tttttatcga ggcgccgaaa
    17941 ccattttgcg gaaactcgat gtaatcgccg gaaaaagcca gctcagtgaa acctttttgc
    18001 cgggcgagaa tgttctcagg caccgaaagc atcgcggcgt cgacaatccc ggcgtcgagg
    18061 gcggcgagcc ggccagcttc ggatgctaca tagacggttg aataatcccg gccaaattcc
    18121 aagccttcgc ggcgcaaggt atatcgagtc gcgaggtcag tcgtagcgcc gggtgagctg
    18181 acggcaattt ttttgaattt gagttgttga aaatttttaa tttctgcttt tgccataagg
    18241 ccctgcacgg gcgaacgcat cgtaaagatg accgccttga tcggctgtga cctgacggcc
    18301 gcagcgacaa cgccggtaac ggcgccattg taatcgatgt caccggtgag ggccgccgtg
    18361 ctagcgaggg cggcgggcat cagaatgaac tcaacgtcga ttccctcatc tttgtaatag
    18421 ccccggtctt tggctgcgta aaacggcaag tacgtcacgt ttgcagctgg aatcgagacg
    18481 cgaactttcc tcagtcccgg ctgagcttgt gaatggctgg cgcaaaccag gactgcggcc
    18541 gtcaacagag cgatcgcaac agctgacgtt cttagaaaag cggccatgga agacctcctt
    18601 tatcatccgg gattgctgcg caatcgtaat gttctcgtca acaaaaagat tccttcgtac
    18661 ttagcacatt ttgatcagcg acatggaatt cttcccccca attaggaagc gtctgcggtt
    18721 actctggaaa gcgcatgtca atcgcgttac ggagtttcac ctccggtgcc gttcgcggcg
    18781 tgagcatctc tgtagattca agcttcagcg cgccttcgac ctaagttctt cttgaagaaa
    18841 gtcttaaccc agggggcgat agtcgcgaag cagttttggg gtgacacctt gctgacctac
    18901 cgcgggcagc tcaattccaa ttgacacgct cccccgatgg atcttataag cgaccggagg
    18961 atctcacatg acgcgatatc tagcagtcat ctatagtttc atttggctcc tggttttgtc
    19021 gctcggcgat ggtcgaggcg cagagcgcgc ccgcggtcaa ccgcatacga tccggattgc
    19081 gtatgtttca ccggtgacca ccatggcgcc gatctggatt gcgagcgaga tcggtgcata
    19141 ccagcgcgaa gggctcgacc cgagaatcgt cttcattgac gcaagagtct ccattgctgc
    19201 ccttgtcgca ggcgaggtcg atgccgtcgt tatctctgcg ccctcagtca tacctgctgt
    19261 cctgagcggc gccaacatcg ctttcatcgc gggactgcac aacaagatga tcttctcttt
    19321 tcacgcgcag ccggatatcc aaagctcggc gcagcttcgg ggaaagattg tcgggacaaa
    19381 ccgccccggc acacccgcag attatggaaa cagagtcgcg ctgacaaaga tgggtttaaa
    19441 gcccaccgac gtgcagctta tgccgctggg aagttcggga gtaatgtggt cggcgttgca
    19501 atcgcgccag gtcgcaggcg tgacgctcgc tccgccttac agctttagag cggatgcgat
    19561 gaagttttcg cgcctggtgg atacctacga caaaccttac cagaatacgg gcgtcgtggt
    19621 tcgcaaagag cgaatccatt cgagcgcgga tacatggctg cgtttgctca gagctctgcg
    19681 gcaggcaaac ctacgctggt atgaagatcc aaagctcgcg atgttcgtgc ttagtaaata
    19741 cactcagcaa tcggatccgg atgtgctaca gaaaacgtac gatttcgaat cgaatccacc
    19801 cgggtttacc aaggacttaa aagtatccga caccggtctt caaggcatcc tcgacttttt
    19861 agcctccacc gttcgccctg aagccggaaa ggcttctccg aaagaattct acgatactac
    19921 gattctggat cggctggaga aatgaatcag cccgaacaa
//
LOCUS       DASUVS010000176         9746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1600421, whole genome shotgun sequence.
ACCESSION   DASUVS010000176 DASUVS010000000
VERSION     DASUVS010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9746
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600421"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1516)
                     /locus_tag="VF182_09730"
     CDS             complement(<1..1516)
                     /locus_tag="VF182_09730"
                     /inference="COORDINATES: protein motif:HMM:NF028512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX6437398.1"
                     /translation="MAGALVNQIYFLDVGSLRRRLQSTRVNSDIVYTLIMDSEGAVLS
                     DGTSANVLRDQKPTDPFTNRLMKENDWISAVEGELLKVAGPVFMPDRSRVGYIIVGFS
                     LQRINEMVHETTKSSLYLTAFAFGIAFLLAFAVAASFSRPILTVARAAKDIGEGRFDT
                     RLSVTRSDELGMLAQSVNLMAETLQSRDAEVRLSVERLEQEIAERNEAEEGLRHSEER
                     FRSLVSVITDVPWVADPTGAFATPQPAWEAYTGQRWEEHRGFGWANALHPDDRDRVKE
                     LWKEACATRALYYCEERLWYAPRQQWRFVVAKATPLINANGSVREWVGTCTDIHDQKR
                     AQEWLETTVAQRSSELHQANAALLRDMEERHKLQEQLLHAQKMESMGTMAGGIAHDFN
                     NILNIIQGYASLLQGYGAHNKEIAESLSVIHKSVQRGSTLVQQLLTLARKTTTDFNPV
                     NTNNLIEELIALISQTFPKTIALSTTLESHLPPITADENQIEQALLNLCVNARDA"
     gene            complement(1663..2697)
                     /locus_tag="VF182_09735"
     CDS             complement(1663..2697)
                     /locus_tag="VF182_09735"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6437399.1"
                     /translation="MFIADVTSILLVTDHNTFVDLETSRHALTKLLIHPELNPAGGVA
                     MFHSFRFQATAVLLMAGFICPYFAPAEPLVIGSIHHEVTSEIKKFLPLADYLGKHLQS
                     EEVNEAKIVVAKDVFQMGTLLQKRKVDIYIDSFFPSVAANLLSGSKFLLRRWKRGRPE
                     YRSVIYHKKDSNLHQVESLKGHILAFEEPFSTSGYFIPKIALVQAGLKLTPKPDAAKS
                     TDPQEIGYVFSLDDENTVAWVLAGKVSAGATDDYTYQRQTHRYPNSLKAIHTTSSFPR
                     HLVSHRADLAPKLVTKIKAILIQMDQSEEGRRALKDFERTTMFDELPSASTAQLLNVR
                     KFVEAELRLR"
     gene            3089..3451
                     /locus_tag="VF182_09740"
     CDS             3089..3451
                     /locus_tag="VF182_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6437400.1"
                     /translation="MTKILLVEDHSELRHVLRDRIEGLGFTTITATNGKEGVDTAVAE
                     KPDLIMMNSMMPEMDGWEAARILRSNPETKDIPILAATAMFQPAHLKAWLDVGCSDYI
                     IKPFTLDELSRKIRGLIG"
     gene            3766..4770
                     /locus_tag="VF182_09745"
     CDS             3766..4770
                     /locus_tag="VF182_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437401.1"
                     /translation="MLTKIRLLLIALAWIVPASLSAAERHIHTSAGLGGSGLPLYVAK
                     SAGIFEKYGLDVDLLVIPGGPLGMQTLLSGNTQSANIAAMTPIRTVLAGGDIVMVGGH
                     LNRQMFNLVTRKEIRKPEDLRGKKIGVVSLAGTNAVAVRLVLKEWKIPFEAVNLLVVG
                     GSPTQLAALESGTIDAAVLTYGITKEALRKGMTVLADLSKPVPEFPDRTIIMRRSFLM
                     KERNHVKHFMQGLSEAIYQIRTADNRRREEFIAIAGKHLRVNPTAAREFYDLYENAFS
                     FPPRVGRNGMAAVIDLIQEETGRPKVEFELNRFLDESVLDELEREGFFKKLESEHARR
                     "
     gene            4834..5703
                     /locus_tag="VF182_09750"
     CDS             4834..5703
                     /locus_tag="VF182_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6437402.1"
                     /translation="MQARIRHVAVMCQDAPRVARFYELLFALAAKAQTSDELHAKMFG
                     APVLGSKRVVRPYERTIIVSDGNIGFAFLRRAPGYPGGIDHFGIEVDDLDTVFARMKE
                     RYPKVGAVKRPANRPFAAYSSHDPEGHLFDLAQHDSQNIRGVWSETPREQERYIKHIT
                     IRAIDPEAMARFYMDVFEFKQEEKALEDPNFYLTDGKVTLVLTPWKIQDYPEAEHRGP
                     GFDHVGFKVESVEAFKKDLETVTSVDPANLAPRAASNIPEQKVVLGLLQSCRYGQYHV
                     PDPEGNYVDVSEH"
     gene            complement(5930..6913)
                     /locus_tag="VF182_09755"
     CDS             complement(5930..6913)
                     /locus_tag="VF182_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437403.1"
                     /translation="MKLRLASAAPASPAKKKRHCLNALAVALFVVASIVSESGQAQPV
                     LSAARPEKPGKPAPLTDINYTVTEAFPHDGKIVAAVNRSRELALVGDSAAFFVDKKRN
                     ITEFECLVFSAGATQLFTTPASINNRGDIVGFCHDDLGQIETGFLRSKNGNLFQFTAP
                     DAASTVAHGVNDFGQIVGRASGTSGSFSFIRSSDGRYQNILPPTIEALVQAVAINNSL
                     KVVGHYSDNNGQHAFLWDNGQFTELRVPGANGNNDVRQLNNNDQALMFATVDGVGSYF
                     LYSDGNYLRINNPEGYTWQSVNALNDNGELTGSAFDDQNVFFNIIARPSKK"
     gene            complement(7244..8311)
                     /locus_tag="VF182_09760"
     CDS             complement(7244..8311)
                     /locus_tag="VF182_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase family protein"
                     /protein_id="HEX6437404.1"
                     /translation="MALPRDILDVQTIYMEPAIEAYDRGRQVLARYPDARLIIVPSHN
                     NIPTLYGNEGNAEDWLHIKQHTLVLGVKKSMSMRPNERSSNFIAPSTSNGCAMACAYC
                     YVPRRKGYANPITVFVNMEQICAALTRHAQRQGVKPRADQVDPKLWVYDLGENGDLSV
                     DALVSDNVRDLIATFRDIPNAKGSFATKYVNRDLLGYDPQRKTRIRFSLMPERVAQVV
                     DVRTSPVSERIAAINDFVDAGYEVHINFSPVIVYDGWQTDYVSLFANLDETLSAGAKE
                     QLKAEVIFLTHNAALHEVNLRWHPRAEDWLWRPELQQKKLSQNGMWNLRYKVHEKRGY
                     LESFLGTLKSALPFCEVRYAF"
     gene            8615..9568
                     /locus_tag="VF182_09765"
     CDS             8615..9568
                     /locus_tag="VF182_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6437405.1"
                     /translation="MPVIPLKPVDRVEVLSVMDNSIDVLMGNTPVARRIKRSRDSHTR
                     PQLRAEHGVSMLVSTYADGNRDSFLFDTGVTLDGVLHNMDVMEIKGNELHAVVLSHGH
                     TDHTRGLMGFIKRYGRPRIPILLHPDAYLKRKNVFPDGHESEHIPPSKRDLEAEDVQI
                     VEDRGPSMLIGGRALVTGQIPRTTGYEKGSPNQVAWIDGKWQPDPWIHDDQAIVLNVK
                     DKGLVVLTGCGHAGVINTLRCARELTGVDQVHAVIGGFHLTGPVFEPVIAPTVEALKD
                     FAPKIIVPQHCTGWRATHMIARHFPDAFVPNSVGTTLVIGA"
BASE COUNT         2315 a         2509 c         2623 g         2299 t
ORIGIN      
        1 tggcatcgcg ggcgttcaca caaagattta gcagcgcctg ctcgatttgg ttttcatcgg
       61 ccgttatcgg cggaagatgc gattccagtg tcgtgctcaa cgcgatggtc ttgggaaaag
      121 tttggctgat cagcgcgatc agttcttcaa ttagattgtt cgtattcacg gggttgaaat
      181 ccgtcgtagt tttccgcgcc agagttaaaa gctgctgtac cagggtggag ccgcgttgga
      241 cgctcttgtg aatgacgcta aggctttccg cgatctcctt gttgtgggcg ccatacccct
      301 gcaggagaga agcgtagcct tgaataatat taagaatatt attgaagtcg tgggcgatcc
      361 cgccagccat tgtacccatg ctctccatct tctgcgcgtg caaaagctgc tcttggagct
      421 tgtgtcgttc ctccatatcg cgcaaaaggg ccgcattggc ctgatgcaac tcgctactgc
      481 gctgggccac cgtggtttcc agccactcct gggcccgctt ctgatcatga atgtcggtgc
      541 atgtgccgac ccactcacgc acactgccgt tcgcgtttat cagcggtgtc gctttggcga
      601 caacgaaacg ccactgctgc cgcggcgcat accaaagccg ctcctcgcag taatagagcg
      661 ctcgggtggc gcaagcctct ttccagagtt ctttgactct gtcgcggtcg tcaggatgaa
      721 gagcattggc ccagccgaat ccacgatgtt cttcccacct ttggccggtg taagcttccc
      781 aagcgggttg cggcgtggcg aaagcaccgg tgggatcagc gacccacggt acgtcggtta
      841 tgactgaaac caacgagcga aaacgctctt cgctgtgccg taggccttct tctgcttcat
      901 tgcgctcggc gatttcctgc tctaagcgtt cgacgctcaa cctaacttca gcatcgcggc
      961 tctgcaaagt ttccgccatt aggtttacag actgcgccaa cataccaagc tcgtcactcc
     1021 tagtgaccga taaccgagta tcaaatctac cctcgccaat atcctttgct gcgcgcgcga
     1081 cagttagaat gggacggctg aaactcgccg cgacggcgaa cgccagaaga aatgcgatac
     1141 caaaagcgaa ggcggtgaga tagagactcg atttcgtagt ctcgtggacc atttcattga
     1201 tcctttgcaa tgaaaaaccc acgataatgt atccgactcg acttcgatcc ggcataaaaa
     1261 ctggcccggc gactttgagc agctccccct ctaccgcaga tatccaatcg ttttccttca
     1321 ttaatctgtt ggtaaatggg tccgtgggtt tctgatcgcg caggacattt gctgaggtgc
     1381 catcggagag aacagcccct tcggaatcca tgatcagcgt gtatacgatg tcggagttca
     1441 ctcgcgtgct ttgtaagcgc cggcgcagtg aaccgacatc gaggaaatag atttgattga
     1501 cgagtgcccc agccatgagc gctgcagttc tccgcgaatt ttgctcaaaa gctgttagca
     1561 tccggcgctg tccgagaaat acggaatgaa gggaaatgct tcctcccacg agcaggacaa
     1621 tggcaaaagt caaaaggacc agcttaaggc ggatattgaa cttcatctga gtcttagctc
     1681 ggcttcaaca aatttccgga cgttcagtaa ttgtgccgtg gatgcgctag gcaactcgtc
     1741 aaacatagtt gtgcgctcga agtcctttag cgccctcctg ccttcctctg actgatccat
     1801 ttgtatcaat atcgccttga ttttagtcac aagttttgga gcgagatcgg cgcggtggct
     1861 aaccaaatgg cgtggaaaag aagacgtggt gtggattgcc ttcaaactat tcgggtatcg
     1921 atgtgtctgc cgctggtagg tgtaatcatc cgtggctccg gcgctcactt ttcctgcgag
     1981 aacccatgcg acggtatttt catcatcaag actgaacaca tagccgattt cctgcggatc
     2041 tgtggacttt gcggcatcag gcttgggagt cagtttcaat cccgcctgta ctaaagcgat
     2101 cttcgggatg aagtaacctg atgtcgaaaa aggctcctca aaggcgagga tatgaccctt
     2161 gaggctctcc acctgatgca agttgctgtc ctttttgtga tagatgacgg agcgatactc
     2221 cggccgccct ctcttccatc ttcgcaatag gaacttgctt cctgaaagga gattcgccgc
     2281 gaccgaagga aaaaaactgt ctatgtagat atccaccttg cgtttttgta aaagcgtacc
     2341 catctgaaaa acgtcttttg ccactacgat tttggcctca ttgacttctt ctgactgcaa
     2401 gtgttttccg agataatcgg caagcggcaa aaactttttg atctccgacg ttacttcgtg
     2461 atgaatagag cctataacaa gcggctcagc cggggcgaaa tagggacaaa tgaagccggc
     2521 catcagcaat accgctgttg cctggaatct aaaggagtgg aacatcgcga ctcctcctgc
     2581 cggatttaat tcagggtgga tcagtagttt agtgagagcg tgacgagatg tttcgagatc
     2641 gacgaaggtg ttgtgatccg taacgagcag aatagatgta acatcggcaa taaacaatta
     2701 cggctgcacg agatctccct caaatgtcgt ttcatttcac acctccggaa agacaaatga
     2761 gccgatagct ccctgacttc tactgccgtt cgctagcgct tgtctagata tttctgtgac
     2821 gaaatcatcg agcatgccta gtgaacacga cgagaaatat ggttcgtata tttaggcacg
     2881 cctcctcgtg tgaagattga ttccgaggta agtcggacaa acagttgaag tagcagaact
     2941 accctcatgt tacagatccg agaaggccac cctatctgga cgatacggtt cggttgacga
     3001 acggataacc gttggctagt ctctagttgc gaaccgcgag tacaaagtga attgatcgtt
     3061 ggcggaaata agcggaatcc gtttcaccat gactaaaatt ctgcttgtcg aagatcattc
     3121 tgaattgcgc catgtcctcc gggaccgaat agagggactg ggatttacta ccattaccgc
     3181 tacgaacggc aaggagggcg ttgatacagc tgtcgcggag aaaccagacc taataatgat
     3241 gaattctatg atgccggaga tggatggttg ggaagccgcc agaatattgc ggtcgaatcc
     3301 tgaaactaag gacattccga tcttggccgc gaccgcaatg ttccagcccg cccatcttaa
     3361 agcttggtta gacgtcggct gcagcgacta tatcatcaaa ccattcactt tggacgaact
     3421 ctcaaggaag atcaggggtc tcattggctg aagtgttccg aggcgagccg gagccgcaca
     3481 agatcttaat tcctcatcat cattttcgca catgagcatc gttgtaggct taccgaaggt
     3541 tgtgtttgaa aaggatgctc ccacttcaag cagtcgttac aaacgtttga cccttcgctc
     3601 agttgatgtt gtaagtcaag gcgttcgcgc tcgcaggata agcggctcgt ccaatggcat
     3661 ccaactattc aactagatcg ttccaatggc gaaccagacc tgacataccg agatagactt
     3721 gacagccgtt cttttgccgc agtagggtcg acgggtaaag gcggcatgtt gacaaaaatc
     3781 cgactgctgt tgattgccct agcttggatc gtgcctgcga gcttgtctgc tgcagaacgt
     3841 cacatccata ctagcgctgg gttaggcggc tcaggtttac ctctctacgt tgcgaaaagc
     3901 gctggcattt ttgaaaaata tggactggac gtcgacttgc tcgtcattcc cggcggaccg
     3961 ctcggaatgc agacgcttct tagcggtaat actcagtccg ctaatatagc ggccatgact
     4021 cccatcagaa cggttctggc aggcggcgac atcgtgatgg tcggggggca tcttaaccgt
     4081 cagatgttta acctcgtcac aagaaaagag atccgtaaac ccgaagacct gcgcggaaaa
     4141 aagattgggg tcgtcagcct ggcaggaacg aacgcggtcg ctgttcgctt ggtgctcaaa
     4201 gagtggaaga ttccgttcga ggcggtgaac ctgctcgtgg ttggcggaag tcctacacaa
     4261 ctggccgctc tcgaatccgg tacgattgat gcggccgtgc ttacgtatgg cataaccaag
     4321 gaagccctgc gcaaaggaat gaccgttctg gccgatctat ccaagcctgt cccggaattc
     4381 cccgatcgga cgattatcat gcggcgctcc ttcttgatga aagaacgaaa tcacgtcaaa
     4441 cacttcatgc agggactcag cgaagctatt tatcagatta gaaccgccga caatcggcgg
     4501 cgggaggaat ttatcgcgat tgccggaaaa cacctgagag taaacccgac ggcggcgagg
     4561 gagttctacg atctctatga aaatgctttt tcctttcccc cgcgtgtcgg ccgaaacggg
     4621 atggcagcgg tgatagatct gattcaggag gaaaccggac ggccgaaggt ggagtttgag
     4681 ctgaaccgat ttcttgacga gagcgttctc gatgaactcg agagagaagg cttctttaaa
     4741 aagttggagt cggagcatgc gcgaagataa acagcgtgtt ttcaaaattg ataggagcgc
     4801 gcctctatct gcgcgccaaa taggaggtcg atcatgcaag ctagaatcag acacgtcgca
     4861 gtaatgtgcc aggatgctcc tcgtgtcgcc aggttttacg agctgctgtt tgcgcttgcg
     4921 gccaaggcgc aaacttcgga cgagcttcat gccaagatgt tcggcgcgcc ggttttagga
     4981 tcaaaacgag tcgtacggcc ctacgagcga acgatcatcg tgagtgatgg aaatatcggt
     5041 tttgcttttc ttagaagggc gcccggttat ccaggcggca tcgatcattt tggcattgag
     5101 gtcgatgatc tcgatacggt gtttgcgcgc atgaaagagc gctatccgaa agttggcgcg
     5161 gtaaaaaggc ccgccaatcg acctttcgca gcctacagtt ctcatgaccc ggaaggtcat
     5221 ttattcgatt tggcgcagca cgattcgcaa aatataagag gcgtttggag cgagacgcca
     5281 agagagcagg agcggtacat caaacacata accattcgcg cgattgaccc tgaggccatg
     5341 gcccgatttt atatggacgt gttcgagttc aagcaggaag aaaaggcttt ggaggatcca
     5401 aatttttatc tcaccgacgg caaggtgact ctggtcctca ctccatggaa aattcaagat
     5461 tatcccgagg ctgagcaccg aggcccggga ttcgatcatg taggtttcaa ggttgaaagc
     5521 gtggaggctt tcaaaaaaga tctggaaact gtgaccagcg tggatccggc gaatctggcg
     5581 cctcgcgcag cgagcaatat tcccgagcag aaggtcgtgc tggggctgct gcagtcatgt
     5641 cgttacggcc aatatcacgt gcccgaccct gagggaaatt atgttgatgt ttcagagcat
     5701 tgaaacagtt ctcttgccgt tgcttagaag ctgatattca agaggagggt gcggtagcgc
     5761 gacgcgcggt tcggtaattg aactcgtcgc ctccgaagaa ctagttccga gtacgttgac
     5821 ctctttgcca gttattgcga gatagcgcga tattcaagat accaagcaga aatcgtattt
     5881 aggaagaaac cgcccggctt tgcttctagt atgtgactga gttgcaaaat tacttcttgc
     5941 taggtctggc aatgatgttg aaaaaaacgt tctgatcatc gaaggcagaa ccggttaatt
     6001 ccccgttatc gttcaaggcg ttgacacttt gccaagtgta gccttcagga ttattaatcc
     6061 gcaggtaatt tccatcactg tatagaaagt aagatcctac gccgtccact gtcgcaaaca
     6121 tcagtgcctg atcattattg ttgagttggc gaacgtcgtt gttaccatta gcaccgggca
     6181 ctctcagctc ggtaaactgt ccgttatccc atagaaatgc atgttggcca ttattatccg
     6241 agtagtgacc gaccactttt aagctgttat tgattgcaac agcctgtacc agagcttcga
     6301 ttgttggcgg caaaatattt tgataccggc catcgctgct ccgaatgaaa ctgaaactgc
     6361 cggaagtacc cgacgcccgc ccgacgatct gcccaaagtc attgacgccg tgcgccaccg
     6421 tcgatgcggc atcaggcgca gtaaactgga acagattgcc atttttgctg cgcaagaaac
     6481 cggtttctat ctgacccaga tcatcatgac agaatccgac aatatcgccg cgattgttga
     6541 tcgaggcagg cgttgtaaaa agttgagtgg caccagcgga gaacacgagg cattcaaact
     6601 ctgtgatgtt gcgctttttg tcgacaaaaa aagcggcaga atcgccgacg agcgccaatt
     6661 ccctcgatcg gttaaccgct gctacgattt taccgtcatg agggaaggcc tcggtgacgg
     6721 tgtagttgat gtccgtaagt ggggccggct tccctggctt ctcgggccgt gcggcggaca
     6781 gaacaggctg cgcctgccca ctctctgaca cgattgatgc gacgacgaaa agagctacag
     6841 ccaaggcgtt tagacaatgc cttttctttt tggcaggcga tgcaggagca gcagaagcaa
     6901 gtcgcaattt catggacacc tccagaggcg gttgattatg ccaagcacat ctctccgggc
     6961 gcgaattctg tgatttactc tcagcgtata cgaacatttg ggaccaagac accaatgatt
     7021 acctaaaatt tacaaaaatt gctatgaaat gcccataact gcatagcacc gcaactaacc
     7081 tagcgtcaac aggattcttg cgcagcttgc ggcttctcaa acgaaaacag ctctgggggt
     7141 ggggccgccg cggtggcggg agagacgggc gcggagatgg cccgtctact ttcaactcag
     7201 gccgcttatt tctttaaaga tcgagcgccc gcacatctgc agcttagaag gcgtaacgca
     7261 cttcgcagaa agggagagcg ctttttaatg tgccgagaaa agattccaga tacccgcgct
     7321 tctcatgcac tttatagcgc aggttccaca tgccgttctg gctaagcttc ttctgctgca
     7381 actccgggcg ccagagccag tcctcggctc ggggatgcca gcgtaagttt acttcgtgca
     7441 gagccgcatt gtgcgttaga aaaatgactt ctgccttgag ctgctctttg gccccggcgg
     7501 agagtgtctc atctaaattc gcgaagagag agacgtaatc agtctgccag ccgtcataga
     7561 cgatcacggg ggaaaaattg atatggacct catatccggc atcgacgaaa tcgttgattg
     7621 ccgcgatccg ttcgctcaca ggcgatgtgc gcacatcgac aacttgggca acccgctcgg
     7681 gcatcaagga aaagcgaatg cgggtcttgc gttggggatc gtagccaagc agatcgcgat
     7741 tgacgtattt ggtcgcgaaa ctgcccttgg cgtttggaat gtcgcgaaag gtggcgatga
     7801 gatcgcgcac gttgtcggag accagggcat caacggacaa atcgccattc tcgccgagat
     7861 cgtagaccca gagtttcgga tcgacctgat ctgctctcgg cttcacgccc tggcgctgtg
     7921 cgtgtcgcgt caacgcggcg cagatctgct ccatattgac gaaaacagtg atcggattgg
     7981 cgtaaccctt acgccgcggc acatagcagt aggcgcatgc catcgcgcag ccattggacg
     8041 ttgacggcgc gataaagttg gagctgcgtt cattgggccg catactcatg ctcttcttca
     8101 cgccgagtac cagggtgtgt tgcttgatat ggagccagtc ttcggcgttg ccttcattgc
     8161 cgtaaagggt agggatgttg ttatgcgaag gaacaatgat gaggcgcgca tccggatagc
     8221 gcgcgagcac ctggcgaccc cgatcataag cttcgatcgc cggctccatg tagattgttt
     8281 gaacgtcgag gatatcgcgg ggaagcgcca tgtctaagaa tcagtattgc taccggcagc
     8341 ttcaacttca acagtgtgcg tcaataccat ccggatcgcg atgttatggc cagcgattgt
     8401 atgccaggcg aacatccaaa atccgcaggt gagaaaattt cgctcagctc gaagatctct
     8461 tgatgaagag caggacagac acacctgaat tggctttatc gcacgaagtt gagatgccct
     8521 actgccgctg gcttctcatt aacccgatct cctttagttt ccttgacgcc tttgcgcggt
     8581 ttcggctagc gtgccggcaa agatggaggt gccgatgcct gtcatcccgt tgaaacctgt
     8641 ggatcgggtc gaggtgttga gcgtcatgga taattccata gacgtgctca tgggaaacac
     8701 gccggtggcg cggcggatca agcgctcgcg tgactcgcac acacggccgc aacttcgggc
     8761 cgaacacggc gtctcgatgc tggtgtcgac ctatgctgac ggcaacagag atagctttct
     8821 cttcgatacc ggcgtgacac tcgacggcgt tttgcacaac atggatgtca tggaaatcaa
     8881 aggtaacgag ctgcatgccg tggtcctgag tcacggtcac accgaccaca cccgcggctt
     8941 gatgggattt atcaagcgtt atggccgtcc gagaatcccg atcctgcttc atcccgacgc
     9001 ctatctgaaa agaaagaacg tctttcccga cggccatgaa tccgagcata tcccgcctag
     9061 caagcgcgac cttgaggccg aagacgtgca gatcgtcgaa gaccgcgggc catcaatgct
     9121 gatcggcggt cgcgccctgg tcacggggca gatccctcgc accacaggct atgaaaaagg
     9181 cagcccgaac caggtggcct ggattgacgg aaagtggcag cccgatccct ggatccatga
     9241 cgatcaggcg attgtcctga atgtgaaaga caaggggctg gtggtcttaa ccggctgcgg
     9301 ccacgccggc gttatcaaca cgctcagatg cgcgcgcgaa ttgaccggtg tagatcaggt
     9361 ccatgcggtc atcggcggat ttcatctgac cgggcctgtg ttcgagccgg tcatcgcacc
     9421 gacagtcgaa gccctgaaag atttcgctcc taaaatcatt gttccgcagc actgtaccgg
     9481 ctggcgcgca acccacatga tcgcgcgaca tttccccgat gctttcgtgc cgaatagtgt
     9541 gggcacgacc ctggtaatcg gcgcctaagc ctctgcgcgt ctcggtcagg tttacatcca
     9601 gtcacatgtt cgcgggcctt actcccttca cacggcgttg tttcgaacgg caaagtatta
     9661 aagggcgtcc cactgagcct ccagcccgac cgcgtcgttc ggcgcgcgac agggtttcat
     9721 tcagtgaaag agcagataaa gttagt
//
LOCUS       DASUVS010000177         4159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_289903, whole genome shotgun sequence.
ACCESSION   DASUVS010000177 DASUVS010000000
VERSION     DASUVS010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289903"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..909
                     /locus_tag="VF182_09770"
     CDS             334..909
                     /locus_tag="VF182_09770"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEX6437406.1"
                     /translation="MKIHASLGLLLTLILIAEPNNLPAANESIFSSEDRSLTPENIGR
                     RLRELLDRVKENFSFEISAEDRAPISLMLRNRERLGLTADQVKHLEQLRNDFAKESIR
                     SEANLRIAELDLTSLLEAQPVDMARVEAKIREIERIRADLRIARVRSIEKGKAQLSAE
                     QRRKLQELEADQNFAGSRFKNEPSTWFKESK"
     gene            complement(956..1933)
                     /locus_tag="VF182_09775"
     CDS             complement(956..1933)
                     /locus_tag="VF182_09775"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437407.1"
                     /translation="MKIRTFTIMSVLLLAFFSFQSAVLAQSGPERIRISYSSGGMTSI
                     DLFIARDRKYFQQQNLSSELIRMSANLAISAGISGDVDVLGSIGSAIRSIQRGTPLRV
                     ISVTLRRPLFFLVARPEYSTVKDLKGKTLGIVTFGGSQHTTAKRLIALHGVNADKELT
                     SIQIGEESMQLQALTTNAIQVAAISPPYAQIARDKFNMKILETSTDKFASIQNGAAVN
                     VKMLQERPDFLKRFLRARAQANRYFLEREKEASELLATIWNSDFKIANESYRASKAAF
                     TGTGIPTDDEIKEYLALDAQIIGLPQPVPAASVFDFTLQREINKELGVK"
     gene            complement(2047..3474)
                     /locus_tag="VF182_09780"
     CDS             complement(2047..3474)
                     /locus_tag="VF182_09780"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6437408.1"
                     /translation="MPDLRTWLSEVDKLGQLITVENVDWDLELSTLTEIINEQSKIRP
                     AIVFDRIKGYPDGYRVAVNLLSSLQRLALTMGMDPGISEFEFVQQWRKQVKSIQPIEP
                     RKVKQAPVFENVQKGGDINLLKFPVPRWHELDGGRYVGTDDLVVTRDPEENWVNVGTY
                     RIMVHGRDHLGLHMSPGKHGRVHRDKYHQAGKPLPIAVSFGHHPINFLVASTDVPNRI
                     NEYAYAGGITGNALDIIDAPLTGLPLPADAEIALEGEVLPNDFMPEGPFGEWTGYYAS
                     NQPAVPVIRVKAVYHRNDPILCGFPLLKPSSGDNLHFSLMRSALIWNALDEAGVPDVQ
                     AVWAHPSGGRFMTVLSIRQRYPGHAKQAAMIASQCRSGAYLGRYVVVVDDDIDITNSE
                     EVIWAISSRSDPAQSIDILRRCWSGPLDPRIPAEEKGFSSRAIIDATRPYEWRDKFPK
                     VSGASRELKSEISKKWNGFLDDKIR"
     gene            complement(3608..>4159)
                     /locus_tag="VF182_09785"
     CDS             complement(3608..>4159)
                     /locus_tag="VF182_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6437409.1"
                     /translation="GNKVQTIRENAGDHLQRRAGVVRPTWENTKRENLPLRYAWKDIE
                     PQLRAFVDQPGSPYDGVTLEYVNPVTGGHTLRTLSCWIQLLRPGERTKKHRHTSSAAY
                     FVVAGEGTTIVGDKALEWNKHDCFAVPNWAWHEHLNRSKSEEAILFSVNDIPIVEAFN
                     LYREEPENSLHTTQVPQVPAIER"
BASE COUNT          920 a         1134 c         1133 g          972 t
ORIGIN      
        1 gcgagaactt cttcgtccgg tagaatgctt cgcccgcaag cagcgggctt tgatgacccg
       61 gtggagtttc gtcttgcata gcaaagacct cccttccata ggggcctact cttcgttccc
      121 cctgcgccga gccgcgctca gtgcactcaa tattaatctg ttacctacga gattatttgt
      181 caagcgaatt ttttattaaa tcaaaaattc ttttttgtgc gaaatttgga ctgctgaatc
      241 ggtggcaaca cttagttggt gcgccggagg gaggtttgac atcgggcgcg tagaagctca
      301 gatttaactt cgtcgactgc ccaaggaggg gccatgaaaa tacacgcaag cttaggattg
      361 ctgttgacgt taattttgat agcagagcct aataacctcc cggcggccaa tgaatcgatt
      421 tttagctcag aggatcgaag tttaacgccg gaaaacatcg gccgcagatt gcgcgaactg
      481 ctggaccggg tgaaagaaaa tttttctttc gaaatttcgg cggaagaccg agcgcctatt
      541 tcactgatgc tgcgcaatcg cgaacggctt ggtttaaccg ctgatcaggt taaacacttg
      601 gagcaactgc gcaatgattt tgccaaagag tcgattcgaa gcgaagcaaa tctgcgcatc
      661 gcggaattgg atctgaccag tttgctggag gcgcaaccgg tcgacatggc acgagtcgag
      721 gccaagatcc gcgaaatcga acggattcgc gccgatttac ggatcgcgcg cgttcgatct
      781 atcgaaaaag gaaaagcgca gttgagcgct gaacagcgcc gcaaactaca ggagttagag
      841 gcagatcaga atttcgccgg cagtcgattc aagaatgaac cgtcaacatg gttcaaggag
      901 tcaaaataga ttcggcgatg ggtctcgcgc gctgccacgg cattcggcaa tctactcact
      961 tgactcccag ctctttattg atttctcgct gcaaggtgaa gtcgaagacg ctcgccgccg
     1021 gcaccggctg cggcaggccg atgatttgcg cgtcgagcgc aagatattct ttgatctcat
     1081 catcggtggg aattccggtg ccggtgaatg cggcttttga ggcccgataa ctttcgttgg
     1141 caattttaaa atcggaattc cagatagtgg cgagaagctc gctggcttct ttttctcgct
     1201 ccaaaaaata cctattcgcc tgagcgcggg cgcgcagaaa gcgttttaga aagtccggcc
     1261 tctcctgcag catcttcaca ttgacggcgg cgccattctg gatgctggca aatttgtccg
     1321 tggacgtctc gagaatcttc atgttgaact tgtcccgggc aatttgcgca taaggcggac
     1381 tgatcgccgc cacttggatc gcattcgtcg tcaaagcctg cagctgcatc gactcttcgc
     1441 caatctgaat cgaagtaagc tccttgtcgg cattaacgcc gtgcagcgcg atcaagcgtt
     1501 tggcagtggt gtgctggctg ccgccgaagg tgacgatgcc cagcgtcttt cccttgaggt
     1561 ctttcaccgt gctgtattcc ggccgggcga cgagaaagaa cagcggccgg cgcaaggtta
     1621 ccgagatcac ccgcaaaggc gtgccccgct gaatcgaacg gatcgcgctg ccgatggagc
     1681 ccagtacatc aacgtctccc gaaatgcctg cactgatggc gaggttcgcc gacattcgaa
     1741 tcaattcgct gctgagattc tgctgttgga aatactttcg atctctagcg atgaagagat
     1801 cgatggaggt catcccgccg ctggaataac taatccggat ccgctcgggc ccgctttgcg
     1861 ctaggacggc agattggaaa gagaaaaacg cgagcagcaa aactgacata attgtgaaag
     1921 ttcgaatctt catgctttca tccgcacctg tcaaaataaa attctccgac aaagcacgcg
     1981 cttggactgt actcgtcagg gttggctaat ctcgaaccgg atgtctcgtc gcccatagct
     2041 cttcgttcag cggattttgt catcgaggaa gccattccac ttcttgctta tctcactttt
     2101 taattctcga ctggcgccgc tcactttggg aaatttatcg cgccattcgt atgggcgggt
     2161 ggcatcgata atggcgcgcg agctgaagcc cttctcttcg gcgggaattc gtggatccag
     2221 cggcccgctc cagcagcgcc gcaaaatgtc gatggactga gccggatccg agcgcgaact
     2281 gatcgcccag attacttctt ccgaattggt gatatcgata tcatcgtcaa cgacgacgac
     2341 gtaacggccg agatacgcgc cggagcggca ctggctggcg atcatcgccg cttgtttggc
     2401 atggccagga taacgctggc ggatcgacaa taccgtcatg aagcggccgc ccgacgggtg
     2461 cgcccatacc gcttgcacgt ccggaactcc agcttcgtcc agcgcgttcc agatcaacgc
     2521 cgagcgcatc agcgaaaaat gcagattgtc gccgctggaa ggcttcaaca atgggaagcc
     2581 gcatagaatc ggatcattgc gatggtagac cgctttgaca cgaatcaccg gcacggctgg
     2641 ttgattgctc gcgtagtagc ccgtccattc gccgaacggc ccctcaggca tgaagtcgtt
     2701 tggcagcacc tcgccttcaa gggctatttc ggcatcggcg ggcagcggca atccagtcag
     2761 cggtgcatcg atgatgtcga gcgcgttgcc ggtgatgccc ccggcataag cgtactcatt
     2821 gatccggttg ggcacgtcgg tcgaggccac cagaaaattg atcgggtgat gaccgaagga
     2881 aactgcgatc ggcaagggct tgccggcttg atgatatttg tcgcgatgga cgcgcccatg
     2941 ttttcccggc gacatgtgca gaccgagatg atcccgtccg tgcaccataa tccggtaggt
     3001 gccgacattg acccagttct cttcgggatc acgggtaacg acgagatcgt ccgtgccgac
     3061 gtaacgcccg ccgtcgagct catgccagcg cggcaccgga aacttgagaa gattgatgtc
     3121 accgcctttt tgaacatttt caaacaccgg cgcctgctta actttgcgcg gctcgatcgg
     3181 ctgaatcgat ttgacttgct tgcgccattg ctgaacgaac tcgaactcgc tgatgcccgg
     3241 atccatgccc atggtaagag cgagacgttg aagcgacgat agcaggttga cggcgacgcg
     3301 atagccgtcg ggatatcctt tgatcctatc gaatacgatc gccggccgga tcttggattg
     3361 ctcattgatg atttccgtca aggtcgatag ctccaaatcc caatcaacgt tttcaacggt
     3421 aatcaattgc cccagcttgt cgacttcact gagccatgtt cttaaatccg gcatgggtat
     3481 tcgcctttta agagttcaag tagtccaagc cgttcaaccg cttcgctccg ttcaatcccc
     3541 ctccttcatc ctcccccgcg acgcgaggga ggaacagagg tgggggttgg aacaactgga
     3601 acaattatca tctttctatt gccggcactt gcggcacttg cgtcgtatgc agagaattct
     3661 ccggctcctc gcgataaaga ttaaaggctt cgacgatcgg tatatcattc acggaaaaca
     3721 agatcgcttc ttcgctcttc gaacgattga gatgttcgtg ccacgcccaa ttgggaaccg
     3781 cgaagcagtc atgtttgttc cattcaagtg ccttgtcgcc gacgatggtg gtgccctcgc
     3841 cggcgaccac gaagtaagcc gcgcttgacg tatgtcgatg ttttttggtt cgttcgccgg
     3901 gccgcagcag ctgaatccaa cagctaagcg tcctgagcgt gtgcccgccg gtaacgggat
     3961 tcacatattc cagcgtaacc ccatcgtagg gactgccggg ctgatcgaca aacgcgcgca
     4021 gctgcggctc gatgtctttc caggcataac gcagcggcag attctccctc ttggtgtttt
     4081 cccatgtggg tcgcacgacg ccggcacggc gctgcaagtg atcgccggca ttttcgcgga
     4141 tcgtctgcac cttgttacc
//
LOCUS       DASUVS010000178        24378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1600945, whole genome shotgun sequence.
ACCESSION   DASUVS010000178 DASUVS010000000
VERSION     DASUVS010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 24378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24378
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600945"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VF182_09790"
     CDS             complement(<1..369)
                     /locus_tag="VF182_09790"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX6437410.1"
                     /translation="MVNQFTLIKKDSTSKARLGRLETAHGMIETPCFMPVGTQGTVKA
                     VLPRDLKEMGCQILLGNTYHLHVRPGADLIRQLGGLHKFMGWDGPILTDSGGFQVFSL
                     GAMRKLSEEGVRFQSRLDGSY"
     gene            complement(452..1486)
                     /gene="queA"
                     /locus_tag="VF182_09795"
     CDS             complement(452..1486)
                     /gene="queA"
                     /locus_tag="VF182_09795"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005338735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX6437411.1"
                     /translation="MDVQEFSYDLPESLIAAFPSGNREDSRLLVIRRRSGELIHTRFS
                     ELGNFLDPGDLLVLNDTQVFPARLHGAKESGGKVEVLLLERFPDERSLWIALVNAAKK
                     PKVGSRLIFADMSAEVIGDIGRGRFGLEFHHSGDFETRLAAIGEPPLPPYVRRRRAAA
                     GVDWQRYQTIYASHPGAIAAPTAGFHFTPELLDTLEAKGIQRALLTLHVGPGTFEPIR
                     TEQIESHRMEGERYVLPAEAAAKIQRAKDQARRVVAVGSTTTRTLEWVALQKGRIEAD
                     EGIARLFIRPGDRFRTIDALITNFHLPRSTPLLIVTAFAGLELIRKAYAEAIQKRYRF
                     YSYGDAMLIL"
     gene            complement(1569..2327)
                     /locus_tag="VF182_09800"
     CDS             complement(1569..2327)
                     /locus_tag="VF182_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX6437412.1"
                     /translation="MFGIGAPITFLIIIIVAIISGVKVLKEYERGVVFRLGRMVGARG
                     PGLVYIIPGIEKMVKMDLRTVTMDIPPQDVITRDNVSVKVNAVLYFRVLDPNLAVREV
                     ENYLFATSQLAQVTLRSVCGQGELDELLAEREKINTRIQDILDKQTDPWGIKVVLVEL
                     KHIDLPQEMQRAMAKQAEAERERRAKVIHADGEFQASQKLLDAAEVMSKQPMALQLRF
                     LQSLVEIASEKNSTTIFPVPIDLLTPFLNKSNQT"
     gene            complement(2341..3660)
                     /locus_tag="VF182_09805"
     CDS             complement(2341..3660)
                     /locus_tag="VF182_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX6437413.1"
                     /translation="MKTVRFFFFVSLMAFGLSVLAQPNNSPAPHVDLIVIDGSINPAV
                     DDFIRESINRAISSEARALVIQLDTPGGLLSSTRTIVKEMLSAKIPIMVYVSPSGAGA
                     GSAGVFITMAAHIAAMAPATNIGAAHPVAGGGQEVKGVMGEKIENFTASFSETIAQKR
                     GRNTEWAIEAVRRSVSITETDALKKKVVDIIAKDIDDLLRQADGRKVDLDGRMTTLSV
                     KGVNVVRHEMSLKQKIVNAIADPNIAYMLLMAGILGLYMEFSNPGVMFPGITGAICLV
                     MALISLQLLSFNYAGLFLILLGIALLVGEAFMPSFGILGIGGVISLALGSFLLFDTPN
                     SDLTIDATIVYTAVATMAALVLAIGYLVFRSQKAKPTLGMDGLIGEIGEVRAKLSPSG
                     KVFVHGEYWNAQTQSDGQIDVGEKVQVIGYDGMNLKVKRLQEQGFVA"
     gene            complement(3657..4235)
                     /locus_tag="VF182_09810"
     CDS             complement(3657..4235)
                     /locus_tag="VF182_09810"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX6437414.1"
                     /translation="MSSQPGNDNVSSLEELRGEIGDCRRCKLHLGRTHVVFGTGNPNA
                     NLMFVGEGPGRDEDLKGEPFVGRAGQLLTDIITKGMGLAREDVYIANVVKCRPPQNRN
                     PEPDEVITCEPFLKKQIELIRPKIIVALGKFAVQTLLQSKVPITRLRGNWHMYMGIKL
                     MPTFHPAYLLRNPADKKLVWEDIKKVIAEMQK"
     gene            complement(4537..5733)
                     /gene="coaBC"
                     /locus_tag="VF182_09815"
     CDS             complement(4537..5733)
                     /gene="coaBC"
                     /locus_tag="VF182_09815"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX6437415.1"
                     /translation="MTNGKVTNVLLGVSGGIACYKAVELVRLLVNEQYAVQVIMTQGA
                     TQFVAPLTFQTLSGRPVATETFSLTQESEIGHINLADSADIFVIAPATANMIGKITAG
                     IADDLLTTVLMATQAPVLIAPAMNIHMYENPILQENLRKLRRFGYEILEPDEGYLACG
                     YEGKGRLPDPKVILEEIQRLLKKKDLAGEKLVVTAGPSREPLDPVRYISNRSSGKMGY
                     ALARAAARRGADVVLVSGPTGLTPPPGVKLKAVNTAAEMRQAILDEYARCTAVIMAAA
                     VADFHPAEFSSDKIKRQTGPLDLRLEPNPDILKELGQRKDGKLLIGFAAETRDLDSNA
                     EKKLREKNLDMIVANDVTKEGSGFDGDTNIATIFDCRGATRVLPLMSKDELADQVFDH
                     FLTLRH"
     gene            6044..6895
                     /locus_tag="VF182_09820"
     CDS             6044..6895
                     /locus_tag="VF182_09820"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEX6437416.1"
                     /translation="MKQYAWALKIRTSSALWLIVGALFCLDASSNYALGQEDYSAFPA
                     ANEPSTFAAASSPLTEQSQNTQLALASALAETAEAGEDFDSDPLEVFNEKMFWFNREV
                     LDRFLLKPAATAWDFVIPDPVQRGIHNIFDNLAVVRRVVNNTLQWKLTGAATELARFT
                     INSTVGLVGFFDVARDQFGIQQRDEDTGQTFGLWGIGPGPYLVLPFLPPLTIRDGIGY
                     AFDTAMTPYIYFIPWYANAGMTATSTVNERSLNLETFERVAESTIDLYGAVRNAYLQR
                     RAAAIRQ"
     gene            7126..7485
                     /locus_tag="VF182_09825"
     CDS             7126..7485
                     /locus_tag="VF182_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437417.1"
                     /translation="MPRTKKKTAARHTKDRAHEYIDDGTHDEIDDEDLPSDEAGRRKI
                     KRNYEELLGIRIEKLKGRVLNREKINKSIDGLYFICFECKKICHNLDEGWVEEPTTQH
                     NFCSACAKIKGIRSTAA"
     gene            complement(7482..8480)
                     /locus_tag="VF182_09830"
     CDS             complement(7482..8480)
                     /locus_tag="VF182_09830"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX6437418.1"
                     /translation="MKVRHHTTVGEVAVQGSSRNLTWEIGAVEDGDRLETFVRRRLPH
                     LSRRQSAKAIDENAFLLNGRTAAKGQRLKEGDRVIFQGAQHWLFDSPVGIEIAGVKIA
                     YEDDEIILLDKPAGIPTHGFSARDLDTLANFIVARWPELFSVGRSRWEPGLLNRLDTE
                     TSGLVLVAKNQQAFGNLREQFQRRRITKQYIALVWGEIDSSGSIGLPLAHDPSDPSRM
                     QVVNEFGSNPPVPRSWHATTWFRKLATFGEMSLLEAAMRTGVMHQVRVHLAAMGHAIV
                     GDSLYGSEKYRTDLERHFLHASRLEFSHPGNGNPMVVESPLPIELSSFLKRKEIVV"
     gene            8496..9629
                     /locus_tag="VF182_09835"
     CDS             8496..9629
                     /locus_tag="VF182_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEX6437419.1"
                     /translation="MRQKLYSVIIAGGRGTRFWPLSRSRKPKQLLKLFGPKTLLDQTV
                     ERILSLAGSDRTLIVTVAEQLNAVRNELSGLPRKNFLAEPEGKNTAPCIGLAALEVIA
                     RDSDAVMIVLPADHWVSDVPAFRRTLKAAAELAARHDRLVTIGMQPAYPETGYGYIVK
                     GKPLAGDEPSSAYQVKRFKEKPDAKVARQLIRQASLWNAGIFAWKASTLLTLIERYQP
                     QIHDSLTNIAKAAGGKSLAAQATRLGTVIAREYKKMPSISVDHAILEQAASEGRVLTV
                     PGNFGWSDVGSWAAVHQMSPRDANGNAGNGNWLGLDAKNCLIHAPDRLVVVLGMRDAI
                     IVDTPDALLVADLKRSQEVRELVEELKKRGYASYTIDSFRSGR"
     gene            9640..10461
                     /locus_tag="VF182_09840"
     CDS             9640..10461
                     /locus_tag="VF182_09840"
                     /inference="COORDINATES: protein motif:HMM:NF020287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEX6437420.1"
                     /translation="MDINYPKGPLREGEPVVLLDRKDREYLARLDPERPLAIRGGKVC
                     VQELIGRDEGCTVRSSVNEPFLVFRPSLPQLIPNLPRNAQVVYPKDLGPILIWADLFP
                     GARVVEAGVGAGALSMTLLRAIGSSGQLITYEIREDFADMARKTVSKYFGPTPNWRLK
                     IGDIESELEEIDVDRVLLDLPEPWKVIERAWKALKPGGILLSYLPTIIQVKSLVDGLR
                     ADKRFACVETMETMMRFWHIKGMSVRPQHRMIAHTGFLTAARRLANGERDIPRAP"
     gene            complement(10497..10916)
                     /locus_tag="VF182_09845"
     CDS             complement(10497..10916)
                     /locus_tag="VF182_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6437421.1"
                     /translation="MKIKQIYHIGLPCNDLERATKFYTDVLGMKCTKVGFDTESGGPY
                     KDVYGMFPEISRLFMEDGMCLVLFQRPKPIERGDFDEGTSHIALEVSSDDFDKAADEL
                     KKAGVKVLIDQPVVRPTGKAIYFFDPEMNYIQLWSRP"
     gene            11180..11956
                     /locus_tag="VF182_09850"
     CDS             11180..11956
                     /locus_tag="VF182_09850"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6437422.1"
                     /translation="MASFIRAQNVCLVFKAKDREPVTALTNFSLDIAKGEFVSIVGPS
                     GCGKSTFLNIVLGLIEPASGVIELNGTRISGPGQERAMVFQEFGLLPWRSVTTNVELG
                     LELKGIPSAKRAARATELINLVGLKGFENHYPHELSGGMKQRVGLARALATEPEVLLM
                     DEPFAALDAQTRDLMQTELLQIWERSKKTVLFVTHSIEEAAYLSDRVIVMTARPGRTK
                     DLIRIDLPRPRDYEMRLTPKFNDIKLRIWEVLREELTSRG"
     gene            11959..12744
                     /locus_tag="VF182_09855"
     CDS             11959..12744
                     /locus_tag="VF182_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6437423.1"
                     /translation="MAAQAFSEHRRLWLGLGSILFMIVSWEFVLTYIFPVDPFFFTKP
                     SLIGAAFKEQVQGGKLWHDLAVSSQAFLWGFTFAVIVGIPVGLVMGWRKRVEYSLDPF
                     LTALYASPLVALAPLFIIVFGVGVLGKAALVFLLAIFPFIFNTFAGVKSTDALLINVI
                     RSFGGTEKDLYLKVILPSTMPYIIAGSRIAIGRGLVGIIVGEFYAASEGIGFAISQAG
                     DTYRLPDMFVGIIILSLIAIILTELMRRLELTLAPWRATEEGR"
     gene            12745..13542
                     /locus_tag="VF182_09860"
     CDS             12745..13542
                     /locus_tag="VF182_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6437424.1"
                     /translation="MKAKKIFAEHESLIFGTGFIVLLLVLWESVPLWYTLPKGMALFF
                     TTPSRIASAFYQLLLDGTIEKHFYVSAVAFLAGLGLSIIVGLPLGLLMGRSVTLEQLL
                     DPYVTAANATPRIVFLPLLILWFGIGIWSKILIVFIGALFPLLINTYVGVKNVNRVLV
                     NVVRSFGANEWQVMKIVVLPNSVPYIIAGLRLAIGRAILGVVVGEFFGSSHGLGYMIA
                     SAATNYKVDVVFVGVAIFMAMSVLLTLAIKQLESRLAVWRPEAAKSF"
     gene            complement(13575..14573)
                     /locus_tag="VF182_09865"
     CDS             complement(13575..14573)
                     /locus_tag="VF182_09865"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437425.1"
                     /translation="MKKSSQWIVSEIAAVSLLTLMLAIHASAQSVKIGRTVGGSGFHI
                     PSYVGVDKGLFKAEGLDASFIAATAGVLVRATIAKEIEFTPIPGGGSEAMLKGAPLVF
                     FVGQSLISQWTLTTTPDIKRVEDLRGKTLGLGRPGSADYSEIVITLGKHFKMQPGKDY
                     RVISFTGEPDRIAALIQGSIQGGVLSFPHAARAEKEGMKILMRTGDYIPRLGGTFLTH
                     RDVIKEKRDVVKRFTRGIIKAEDYIRTNKKGTMEVIEKYFEIKDPKVVESIYAQVYDK
                     YSPEMPPDLIQDLFESRTTPELGWPQGKPLPDLNQFTDRSIVNEVLKEMGRKPGGK"
     gene            14713..15663
                     /locus_tag="VF182_09870"
     CDS             14713..15663
                     /locus_tag="VF182_09870"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6437426.1"
                     /translation="MNILITGAGLIGAHAARRAVDAGHKIVLYDLSPNRNYIHEIVGT
                     DPVDVVAADIRDLPALLSALEQFKIDTVVHTAGLIGSRVQENSYTGATNNILGTINVL
                     EAARLRQLRRVVYISTFGVYDRSKIDDSVVRESHPIGGHNLYATTKVCSEHLVHAYAA
                     LYKLDTVILRPGGVFGRGFYVGGSTVGKIMRDLALNIIKGDPIILDANAYGLNEYTYG
                     KDVGLAVFLACQKDNLKQRIYNAGTGIVTGPEELAQIVRELSPNVKVTVSGAVKADRN
                     KAIPLDLSVSKAELGYAPRFPLKEALRDYMDELWKEGSRG"
     gene            complement(15681..16391)
                     /locus_tag="VF182_09875"
     CDS             complement(15681..16391)
                     /locus_tag="VF182_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6437427.1"
                     /translation="MKLSVLIPVYNEERTLEEVVRRVRSIELPKEIILVDDGSKDRSR
                     EILTQLQKEGEQAPDPLNEIRVFLQPNNQGKGAAIKVALDHARGDVIIIQDADLEYDP
                     HDYPALLEPIERGLADVVYGTRFAGGGAHRVLFFWHSLGNRMLTLISNMLTNLNLSDM
                     EVGYKMFRGEVLRSIKLESKRFGFEPEVTLKLAKKGYRFYEVPISYYGRTYQEGKKIT
                     WKDGVSALYYMLRFRLMS"
     gene            complement(16388..17173)
                     /locus_tag="VF182_09880"
     CDS             complement(16388..17173)
                     /locus_tag="VF182_09880"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX6437428.1"
                     /translation="MVIEPLTFESRPNLKNPILILSFAGWSDAGSAATTASRYMCDQL
                     LAKRFASIDPEEFYDFSVQRPMVRLNDNKEREIHWPSYDFFYGEGIGAQADFVFGVGM
                     EPQLRWRAFTDTMLRFIRECQIGMLVTLGAYLDEVLYTRPVPLSGFSTDPKLMEQLDL
                     VPSRYQGPTGIIGVLNEVCRKAAIPGLSLWAALPHYLSVSPNPRGTLALLLRLTQWLG
                     IRLDMSPLERAAADFQAKLNEAVSADPKLSAYIRELKKREFEQ"
     gene            17439..18173
                     /locus_tag="VF182_09885"
     CDS             17439..18173
                     /locus_tag="VF182_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6437429.1"
                     /translation="MESKAALITGGARGIGRAVALDLASRGWSVAICYRTSAGAAHEV
                     VDAARRHGTAALAFQCDVSDPSAATAMVHDLGNLWGRIDALINGAGPYHRIPLLEETN
                     EGWHSMFDNNLHPVFYLSKAVVPLMKQRNWGRIICFSMSNAEQLIAQPQLTAHYISKV
                     GVIVLMRSLARLVAPYGITANCIAPGYIDSGSASKEELQAAVKRIPAGYIGTVEETAA
                     VVRFLLSDEARYINGANIQISGAWGV"
     gene            18178..18663
                     /locus_tag="VF182_09890"
     CDS             18178..18663
                     /locus_tag="VF182_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6437430.1"
                     /translation="MTCSEVHTKALELVQQAMNRQMAGEIDDSIRLYRESIALHPTAD
                     AHTYLGWAYSFQGRIDEAIAQCEIAIQLDPQFGNPYNDLGVYLMQQQKFDEAIPWLER
                     AKQAQRYEPRHFPYINLGRVYMTKGMLQKAIEEFRGAVRLNPADTELVQLLEELETKL
                     Q"
     gene            complement(18792..20114)
                     /locus_tag="VF182_09895"
     CDS             complement(18792..20114)
                     /locus_tag="VF182_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HEX6437431.1"
                     /translation="MKVPSIRTKLPGPNAQAIIDKDRQYTAPAYGRVYPLVVKQGRGM
                     VVEDVDENLFLDFMAGIAVANTGHSHPRVVQAIEEQARKFLHICGSDFYYQPMMELAE
                     KLNELAPGTGTKKIFFTNSGTETVEAALKLARYATKRQHVIAFHGAFHGRTMGALSLT
                     ASRSSHRARFSPLLPGVHHAPYAFCHRCPYHLNYGSCDVGCVSYLEQVLFRYEVTPQE
                     VAAIFVEPIQGEGGYIVPPPEYLGMLQDLCRRHGILLVADEIQSGFGRTGKMFACEHW
                     NIEPDILCVGKGIASGMPLGAMIARSEISTWTPGTHGSTFGGNPVACAAALASIAVIE
                     AGLLHNATDVGAYLMEKLIQLKNKHAVIDDVRGLGLMIGVEFARADGNRTPDAALRDK
                     VMKNCFERGLLLLGCGESTLRFCPPLIVTREESTAAAEIFDLAISELA"
     gene            complement(20172..21662)
                     /locus_tag="VF182_09900"
     CDS             complement(20172..21662)
                     /locus_tag="VF182_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6437432.1"
                     /translation="MSEVFKNYIDGKWLPAQAGEIIENRNPANRNDLVGLFPASDQND
                     VDTAVAAAKKAFAGWRLVPAPKRGELLYRVGELLRAHKEEIARIMTREMGKVLKETRG
                     DVQEGIDTAFYVAGEGRRLFGETTPSELPDKFAMSVRAPVGVCALITPWNFPMAIPTW
                     KMFPALICGNTVVLKPAEDTPYTAVKLFEILERAGVPPGVVNLVQGRGEEVGAALVRH
                     RDVQLVSFTGSAAVGREVAAACGQSLKRVSLELGGKNAQIVMEDADLALAVDGALWGA
                     FGTTGQRCTATSRLILHRTIKTDFIDRLVARIEKIKIGDGLDESVEIGPLINQSARDK
                     VQAYMEIGRQEGARVLIGGSSYDTGACANGFFFQPTILDRVTPSMRVAQEEIFGPVLS
                     VIEVPDFDAAIAVLNDTRYGLSSSIYTADVNRAFRAMRDIEAGITYVNGPTIGAEVHL
                     PFGGVKDTGNGHREAGTTVYDIFSEWKSIYVDYSGRLQKAQIDNAE"
     gene            21898..22923
                     /locus_tag="VF182_09905"
     CDS             21898..22923
                     /locus_tag="VF182_09905"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6437433.1"
                     /translation="MRQLSCLFTAAVIGLFALRPAAAAEDFYKGKTIRIVVGFSAGGG
                     FDTYARAIARHMGKHIPGEPAIIVENMTGAGSLIAANHVYKVAKPDGLTIGHFIGGLF
                     LGQVLGQKGIEFDARKFEFIGAPITDHVVCALTKASGITSVEAWMASKTPIKMGGIAP
                     GTSTPDNATRILKEALGLPIQIVSGYKGTADIRLAAESGEIAGGCWGWDSVSVTWQKA
                     LASGDAVVVLQANRRNHPDLPKVPQAIKLAKTEEGRKMIEVGIHGDSDIVRTYTLPPG
                     TPKDRVQLLRKAFDETLKDPEFLADAKKSKLNVDPVDVEEVEKVIAGLFKLDQSLISK
                     LKEILYN"
     gene            22979..>24378
                     /locus_tag="VF182_09910"
     CDS             22979..>24378
                     /locus_tag="VF182_09910"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6437434.1"
                     /translation="MVEMFSALSSGLVQVFNWSTFSLMMIGIAVGFVVGILPGLGGPT
                     AMALMLPFVFKMNAVEAFAFLLGMTAVTATTGDITSVLFGVPGEPTTASTIVDGHSMA
                     RNGEAGRALGAVLMSSLVGAIFAAVALAIAIPIIRPLVLSFGSPEFFMLALLGISFVA
                     SLSGEDQLKGIVAGGIGLLLATIGLDPISGIQRYTFGQLFLWDGIGLVPITIGFYAIP
                     ETIELAVLGTSIAKQEVGKLGGVMEGVKDTFRHFWLVIRCSALGCFIAIIPGMGAATT
                     QWLAYAHAVQSSPNKERFGKGDVRGVLGPGAANNSTLGGSLITTIAFGVPASVTMAIL
                     LGAFIIQGIVPGPDMLIPPPKGKLDLTFSFVWVIILSNIITVAICFLFLKPLAKVTQV
                     RGGIIIPLILVLIYLGAYAEKNAFADMVVVLLFGGLGWIMEKMEWPRPPVLLGLVLGP
                     LAENRLFLSTDNYGMN"
BASE COUNT         5554 a         6718 c         6463 g         5643 t
ORIGIN      
        1 gtaagaaccg tccaggcgcg attggaagcg cacgccctcc tcggaaagtt tgcgcatagc
       61 gcctaagcta aatacttgga agccgccgct gtcggtgagg atcggaccat cccatcccat
      121 aaacttgtgc aaaccaccca attgccgaat caggtccgcg ccagggcgta catggagatg
      181 gtaggtattg ccgagcaaga tttgacagcc catttctttt agatctctcg gcaagacggc
      241 tttgacggtc ccctgcgtcc ctaccggcat gaagcaggga gtttcaatca tgccgtgagc
      301 ggtttccaaa cggcccaacc gagctttgct cgtcgagtcc ttcttaatca gggtaaattg
      361 attgaccatg cttcgaagtg cgagatgcga aatcttagat ccgatgccat caacgcagca
      421 gatctatcca ccctgccgca tcaacgtgct ttcacaaaat cagcatggca tcgccatagc
      481 tgtaaaatcg ataccgcttt tgaatcgctt cagcatatgc tttacggatc aactctagtc
      541 cggcgaaagc agtcacaatg agcaggggag tcgatcgcgg caggtggaaa ttggtgatca
      601 aagcatcgat ggttcgaaaa cgatcgccgg ggcgtatgaa cagtcgcgca atgccctcat
      661 cggcctcgat ccggcctttc tgcagtgcta cccattccag cgtgcgggtg gtggtcgagc
      721 cgaccgcgac gacgcggcgg gcttggtcct tggcgcgctg aatcttagcc gccgcttcag
      781 ccggaagaac gtaacgctcg ccttccatcc ggtggctttc aatttgctcg gttcgtatcg
      841 gttcgaaagt acctgggcca acgtgcagcg tcaacagagc ccgctggatt cctttcgcct
      901 cgagcgtgtc aagcaactcc ggagtgaagt gaaacccggc ggtgggagcc gctatagccc
      961 ccgggtggga cgcatagatt gtttgatatc gttgccagtc cacgccggca gcagcgcgac
     1021 ggcgccgcac ataaggcggc aaaggcggtt cgccgattgc ggctagtcgc gtttcaaaat
     1081 caccgctgtg atggaactcc aggccgaagc gaccgcggcc gatgtcgcca attacttcgg
     1141 cgctcatgtc ggcgaaaatc aatcggcttc cgaccttggg ctttttggcg gcattaacta
     1201 atgcaatcca aagactgcgc tcgtcgggga agcgttcgag caacagcact tccactttgc
     1261 cgccgctttc cttggcgcca tgcaggcgtg cgggaaatac ctgggtatcg ttaagaacca
     1321 gcaaatcgcc gggatcgagg aaattgccta actcggaaaa acgcgtgtgg atgagctcgc
     1381 cgctcctccg ccgaatgaca agcagccgag agtcttcccg attgccgctt ggaaatgccg
     1441 caatgagaga ttcgggaagg tcataggaaa actcttgaac atccatctca atggagcggc
     1501 aaagcttacc tctcacaaga aaaacgttca gcgccgcgga gtttagattg cggcgcctga
     1561 tttggatctt acgtctgatt agatttgttg agaaacggcg tcaatagatc gataggcacc
     1621 ggaaagatgg ttgtagagtt cttttctgag gcgatctcaa ccaggctctg cagaaagcgc
     1681 agctgtagcg ccatcggttg tttcgacatg acttcggcag cgtccaagag cttttgcgat
     1741 gcttgaaatt cgccgtcggc gtgaatcact ttcgcgcgcc tttcgcgctc cgcttccgct
     1801 tgcttggcca tcgcccgctg catctcctga ggcagatcga tgtgcttgag ctcgacgagc
     1861 acgactttga tgccccatgg atcggtctgc ttatccaaaa tatcctgaat gcgcgtattg
     1921 atcttttctc gttcagccag aagctcgtct aattcccctt gaccgcaaac actgcgcagc
     1981 gtgacttgag ccagctggga agttgcaaaa agatagtttt ccacttctct gaccgctaga
     2041 ttcgggtcca gcaccctgaa ataaagcacc gcattcactt tcaccgacac gttgtctcgg
     2101 gtaatcacat cctgcggcgg aatatccatg gtcaccgtgc gcaaatccat cttgaccatc
     2161 ttctcgatcc ccggaatgat gtagaccaat cctggcccgc gcgcgccgac cattcggcca
     2221 agtcgaaaca caacgccgcg ttcgtactcc ttgagaacct tcacgccact gatgatcgca
     2281 acgatgatga tgattaaaaa cgtgatcggg gcgcctattc cgaacataat acctcctccg
     2341 ctaagccacg aagccttgtt cctgaagccg cttcacttta agattcatac cgtcataacc
     2401 aatgacttga actttttcgc caacatcgat ctgtccgtcc gattgcgtct gggcattcca
     2461 atactcaccg tggacaaata ctttgcctga cggacttagt tttgccctga cctcgccgat
     2521 ctcgccaatc aatccgtcca tacccagcgt gggtttggct ttttgggaac gaaaaaccag
     2581 gtaaccgatc gcaagaacaa gcgccgccat cgtcgctaca gcggtatata caatcgttgc
     2641 atcgatggtc agatccgaat tcggcgtgtc gaatagcaaa aacgaaccta gcgccagaga
     2701 gataaccccg ccgatgccaa gaatgccaaa actcggcatg aacgcttctc cgaccaacag
     2761 cgcaatgccc agaaggatca aaaagagacc ggcataattg aacgaaagaa gctgcaatga
     2821 gatcaatgcc atcaccaggc agatggcgcc ggttattccg gggaacataa ccccgggatt
     2881 ggagaactcc atatagaggc cgagaatgcc ggccatcagc agcatgtagg cgatgttggg
     2941 atcggcaatg gcattgacga ttttttgttt gagactcatc tcgtgccgga cgacgttaac
     3001 ccctttgacg gaaagcgtcg tcatgcgacc gtccagatcc actttacgac cgtcggcttg
     3061 cctcagtaga tcatcgatgt ctttggcaat gatgtcgacg accttctttt tgagcgcatc
     3121 ggtctcggtg atggaaacgc ttcggcgcac cgcctcaatg gcccactcgg tattgcggcc
     3181 gcgtttttgc gcgatggtct cgctaaagga cgcggtgaag ttttcgattt tttcacccat
     3241 cactcctttt acttcctgtc cgccgccggc gaccgggtga gccgcgccga tattggtggc
     3301 cggggccatc gccgcgatat gcgccgccat ggtgatgaaa actccggccg atcccgcccc
     3361 cgcgccgctg ggcgatacgt agaccattat cggaatcttt gcgctgagca tctccttgac
     3421 gattgtccgg gtcgagctca aaaggccccc gggagtgtcg agctgaatga ccagcgctcg
     3481 agcttcgctc gatatggccc ggttgatgct ctcgcgaata aaatcgtcca ctgccggatt
     3541 gatcgaacca tcgatgacga tgaggtcaac gtgcggcgct ggcgaattgt ttggctgtgc
     3601 cagcacagac aacccgaaag ccatcagcga cacaaagaaa aagaacctaa ccgttttcac
     3661 ttttgcatct ctgcaatgac ttttttaatg tcttcccaca ctagcttctt gtcggccgga
     3721 ttgcgtaaca gataggcagg atgaaaagtt ggcatcaact tgatgcccat atacatgtgc
     3781 caattgccgc gtagccgggt aatcggaact ttgctttgca gtaaagtttg tacggcaaat
     3841 tttcccaggg caacaataat cttggggcgt atcaattcaa tttgcttctt tagaaaaggc
     3901 tcgcaagtaa taacttcgtc cggttctgga ttgcgattct gcggcgggcg gcacttgacg
     3961 acgttggcga tataaacgtc ttctcgcgcc aggcccatgc ccttggtgat gatgtcagtc
     4021 agcagctgcc cagcgcgccc gacgaaaggt tcccccttga gatcttcgtc ccgtcccggg
     4081 ccctccccga caaacattag gttggcattt gggttaccag tgccaaaaac cacgtgcgtt
     4141 cggcctaaat gcaatttaca acggcgacag tcgccgattt cgccgcgaag ctcttcaaga
     4201 ctgctcacgt tatcgttacc gggctggctc gacatccgct gcggcgcatt atccagcggc
     4261 tttttagtgt caactgccgc cggcgtaacc gtgccaactt caggtcgcgc cacgaaccga
     4321 acaccccagc gttgttgcct ttgtaaatgc cggcgcagcg aacttatcaa ggttgcgagt
     4381 tcgtcgccgt cattggcaac cataaacaat aattcctttc acgcggagat cttagcaccg
     4441 gagacttgca gctatcaatg acagaatcgc agccgctacg agcgaatcgg cgctgataag
     4501 atcggctgat gatcacgaat actaaaatgg cggtcgttaa tgccttaggg tgagaaaatg
     4561 atcaaaaact tggtctgcca attcatcctt actcatgagt ggcagcacgc gggttgcgcc
     4621 gcgacaatca aaaatggttg cgatattagt atcgccgtca aaaccgctgc cctccttggt
     4681 cacgtcattc gcaacgatca tatcgagatt tttttcgcgc agctttttct cggcgttact
     4741 atccagatcg cgggtttcgg cggcaaagcc gattaagagc ttaccatctt tgcgttgccc
     4801 aagctctttg agaatgtccg gatttggctc taggcgtaga tctagcggcc cggtctgccg
     4861 ctttatcttg tccgatgaaa attcggccgg atgaaaatcc gctaccgcgg cggccataat
     4921 gacggcagta catctggcgt actcgtcgag tatcgcctgg cgcatttcag cggctgtgtt
     4981 aacggccttc agcttaacgc cgggaggcgg cgtgagccct gtcgggccac tgactagcac
     5041 gacgtccgcc cctcgtcgcg ctgccgctcg ggctagcgcg tagcccattt tgcctgatga
     5101 tcggttggag atgtagcgaa ccggatctaa gggttcgcgg ctgggaccgg cggtaacgac
     5161 cagcttttca cccgccaggt cttttttttt gagcagccgc tggatctctt cgagaatcac
     5221 cttgggatcc ggcagccgtc ccttaccttc atacccgcat gcaagatagc cttcgtcggg
     5281 ctcgaggatt tcataaccaa agcggcgtaa cttacggagg ttttcttgca agatcgggtt
     5341 ctcgtacatg tgaatgttca ttgccggcgc aatcagcacc ggcgcttgcg ttgccattag
     5401 cacggtagtt aatagatcgt cggcgatccc agcggtgatt ttaccgatca tattggccgt
     5461 ggcgggcgca ataacaaaga tatcggctga gtcggctaaa ttgatgtggc cgatttctga
     5521 ttcttgcgtc agactgaagg tctccgtagc gacaggccgg ccagatagcg tctggaaagt
     5581 gagcggcgct acgaactgtg ttgcgccctg cgtcatgatc acttgtacgg cgtactgttc
     5641 gtttaccaac aagcggacta gctccacggc cttgtagcat gcgatcccgc cgctgacgcc
     5701 gagaagcaca ttagttacct ttccgttcgt cacgagctga aaatataaaa actattagag
     5761 ccttgcaacc tcaaatgacc gtgagccgta tccgccccga actaaatttg tgacgattac
     5821 accaaagcgt ccgttgattg ttgctctcga tgtgcagcga aggcgttttg agcgtccaca
     5881 aaggattcaa aatcttaaca aaaccccaaa gcatgcgtcg gccccgggtg atctcaatgg
     5941 catgatttta gcgtgaatgg ccgacttacc ggtgaattat aaacgaatgc ttgaccaaaa
     6001 tatcgacatc atgtaagtga caagaatgct aaaggagcgg tggatgaagc aatacgcgtg
     6061 ggcgttgaag atccgaacaa gcagcgctct atggttgatc gtcggggcac tattttgtct
     6121 tgacgcgagt tctaactatg ctctcggcca ggaagattat tcagccttcc cggccgccaa
     6181 cgagccaagc acctttgctg ctgcttcatc gccacttacc gagcagtctc agaatacaca
     6241 actggctctc gcgagcgctt tggctgaaac agcggaagcc ggagaagact ttgactccga
     6301 tcccttagaa gtttttaacg aaaaaatgtt ctggttcaat cgggaagtgc ttgaccgatt
     6361 tttgctgaag cccgccgcaa cggcctggga ttttgtgata cccgatcctg tacaacgggg
     6421 aattcacaac attttcgaca acctggcagt tgtgcggcgc gtcgtgaaca ataccctgca
     6481 atggaagctc accggcgcag ctacggagct ggcgcgcttc accatcaaca gcacggtagg
     6541 gctggttggg ttcttcgatg tcgctcgaga tcaattcgga atccagcaga gagacgagga
     6601 tacagggcag acatttggtc tctggggaat cgggccgggt ccgtacctgg tcctgccctt
     6661 tctgccgcca ctcacgattc gtgacggtat cggttatgcc ttcgatacgg ctatgactcc
     6721 ttacatctac tttatccctt ggtatgccaa tgccggcatg accgcgacaa gcacggtcaa
     6781 tgagagatca ctgaatctcg aaacattcga acgcgttgcc gaatccacga ttgatctcta
     6841 tggagccgtg cgtaacgctt atctgcaaag acgcgcggcg gcgattcggc agtagtgctg
     6901 aggcggaggg attgaagtta tcaggctctc cggccacgct ttctcgctgc ttgagagcgt
     6961 ttgagcagca gcatctatcc acccaaacta ccctactcgg cctccataac cggccatatt
     7021 ctcgccggtt atggaggctg ctcgcctatt gacaacgccg tggtggttgt ttaaaaaaat
     7081 aatcccgcct cagaatcgag aacttcagag gagagagtcg cgaacatgcc caggactaaa
     7141 aagaaaaccg cggcacgaca cacaaaggac cgagctcatg agtacattga tgatggtact
     7201 catgatgaga tcgacgatga ggatctaccg agcgacgagg ctggacgacg caaaataaaa
     7261 agaaactatg aagaactgct cgggatccgt atagaaaagc tcaagggccg ggttctcaac
     7321 cgggaaaaga tcaacaagag catcgacggg ctttacttca tctgctttga gtgtaaaaaa
     7381 atatgtcata atttagacga gggttgggtc gaagagccaa ccacccagca caacttctgc
     7441 tctgcctgcg ccaaaatcaa aggcattcgc agcaccgcgg cctagacaac tatttctttc
     7501 cgcttaagaa agctactcag ctcgatcgga agcggcgatt ccacgaccat cgggttaccg
     7561 ttccctggat gagaaaactc caaacgagag gcatgtaaaa agtgccgctc tagatccgtc
     7621 ctatattttt ccgaaccata gagcgaatcg ccaactatgg catgacccat cgccgcgaga
     7681 tgaacccgaa cctgatgcat gaccccggtc ctcattgcag cctcaagaag gctcatctcc
     7741 ccaaaggtgg caagctttcg aaaccaggtt gtcgcatgcc agctccttgg gaccggcgga
     7801 ttcgatccga attcattgac cacctgcatt cggctcgggt cgctcggatc atgagcaagt
     7861 ggcaatccga tggatcctga ggaatcgatc tcgccccaga ccaacgcgat gtattgtttg
     7921 gtgatccggc ggcgttgaaa ctgctcccgc aggttgccaa aagcctgctg attttttgcc
     7981 accagcacca atcctgaagt ctctgtgtcc aagcggttca gcaacccggg ctcccatcga
     8041 cttcttccca ccgaaaataa ctcaggccat cgcgcaacga taaagttggc cagggtatcg
     8101 aggtcgcgcg ctgagaatcc atgagttgga atgccggcgg gcttatcgag taagattatt
     8161 tcatcatctt cgtacgcgat cttgacacct gcgatttcga tcccaaccgg cgagtcaaag
     8221 agccaatgct gcgcgccctg aaagatcacc cgatcccctt cttttaatcg ttgacccttg
     8281 gctgcggtcc gaccgtttag caaaaaggcg ttctcgtcga tggctttagc cgattggcga
     8341 cgggacaggt gcggtagtcg gcggcgcaca aacgtttcca gccggtctcc gtcttcgacg
     8401 gcgccgattt cccaggtgag attccggctc gatccttgaa ccgccacttc gcctaccgta
     8461 gtatggtgcc gcaccttcat gaatagcatt ctagcatgag gcaaaaactt tacagtgtca
     8521 tcatcgccgg cggcagggga acacgcttct ggccgctcag ccgttcgcgc aagccaaagc
     8581 agctgcttaa gctcttcggc ccgaagacgc tgctcgacca aaccgtcgag cgaatccttt
     8641 ctctcgcggg aagcgatcga acgttgatcg ttacagtagc cgaacagttg aacgccgtgc
     8701 gcaatgagtt gagcgggttg ccgcgaaaga actttctcgc cgagcctgag ggaaagaaca
     8761 cggcgccgtg catcgggtta gccgcattgg aagtgatcgc cagagactct gatgcagtga
     8821 tgattgtttt acctgcagat cactgggtat cggacgtgcc ggctttccgc cgcacgctga
     8881 aggccgccgc tgaactcgcg gcccggcacg atcgcctggt caccatcggc atgcaacccg
     8941 cctatccgga aaccggctat gggtacatcg tcaaaggcaa accactggcc ggtgacgaac
     9001 cctcaagcgc ttatcaagtt aaacggttca aagaaaagcc ggatgccaag gttgcgcgcc
     9061 agctaattcg tcaagcttct ctctggaacg ccggcatctt cgcctggaaa gcatcgactt
     9121 tgctgacgct aatcgagcgg taccagccac agattcacga ctccttgacg aatatcgcca
     9181 aggctgccgg cggcaaatcg ctggcagccc aggctacacg gctcggtact gtgatcgctc
     9241 gtgaatataa gaaaatgccg agcatctccg tcgaccatgc aattcttgaa caggcagcgt
     9301 cggaaggcag agttttgacg gtgcccggca attttggttg gagcgatgtc ggtagttggg
     9361 cggcggtgca tcagatgtcg ccgcgggacg ccaacggcaa tgccggtaac ggaaactggc
     9421 tcgggcttga cgccaagaat tgcctgattc acgcccccga tcgtctggtc gttgtattag
     9481 gcatgcgaga tgcgatcatc gtcgatacgc cggacgcgtt acttgtcgca gatctcaagc
     9541 gctcacaaga ggtgcgcgag ttagtcgaag aactaaagaa aagaggctac gcctcttata
     9601 ccatcgactc gttcagatca ggccgctgac tgtaatctcg tggatatcaa ttatcccaaa
     9661 ggtccattgc gcgaaggcga acccgtcgtt ttgctcgacc gcaaagaccg agagtatctc
     9721 gctcgcctcg acccagaacg gccgctggcc atccgtggcg gaaaagtttg cgttcaagaa
     9781 ttgatcggcc gcgacgaagg ttgcacggtg cgttcttctg tcaacgaacc cttcctggtt
     9841 tttcgcccga gtctgcccca gttgattccc aatttgccgc gcaacgcgca ggtggtttat
     9901 ccgaaggatc tgggaccgat tctcatttgg gccgaccttt ttccgggagc gcgcgtcgtt
     9961 gaagccggcg tcggcgctgg cgctctcagc atgaccttgc tgcgcgccat cggcagctcc
    10021 ggtcaactca ttacttatga aatccgtgaa gattttgccg atatggcgcg aaagacggtc
    10081 agcaaatact ttggtccaac gcccaattgg agattaaaga taggggacat cgaaagtgaa
    10141 ctagaggaaa tcgatgttga tcgcgttctt ctggatttgc cggaaccctg gaaagtgatc
    10201 gagcgcgctt ggaaggccct caaacccggt ggcatcttgc tcagctatct ccccacgata
    10261 atccaggtaa agagtttggt cgatggcctg cgcgccgaca aacgctttgc ctgtgtcgaa
    10321 accatggaga caatgatgcg gttttggcac ataaaaggca tgagcgtccg gcctcaacac
    10381 cgcatgatcg cccatacggg ttttttgact gctgcgcgcc gcttagcaaa tggtgaaaga
    10441 gacattcccc gagctccctg acggcggctc gggcaatgtc aaactcatcg atatgactaa
    10501 ggtcttgacc aaagctggat gtagttcatt tcagggtcaa agaaatagat cgctttgccg
    10561 gtaggacgaa cgaccggttg gtctatcagc actttcacgc cggccttctt cagctcatca
    10621 gccgccttat cgaaatcatc cgagctgact tccagcgcaa tatgcgatgt cccctcgtcg
    10681 aaatcacccc gttcgatagg ctttgggcgc tgaaacagaa ctaaacacat gccatcttcc
    10741 atgaacagcc gggagatctc gggaaacatg ccataaacat ctttgtacgg accgccgctt
    10801 tctgtgtcaa agccgacttt agtgcacttc attcccagca catccgtgta gaacttggtt
    10861 gcccgctcca gatcgttgca cggcaagccg atatggtaaa tttgctttat tttcatgagt
    10921 cacctccttg tgttcctgtc tatactgcaa cgagcgaggg atttccaata gctccaggta
    10981 ctcaaagagc agctggtctt catcggcaat ctccttgaca gatcatcatc ggcaagtcta
    11041 agaatgccga cagaaaaatc acggaccagc ggaataacta gacatcgaca ggatagaaat
    11101 ctatagatga agtcatagac gatagtacag agaacaatag tctaattaag ctggtgcatt
    11161 gcgacacgta agcttttcta tggcatcttt cattagagct cagaacgtct gtctagtctt
    11221 caaagccaag gatcgagagc ccgtcacggc gctgaccaac ttcagcctcg acattgccaa
    11281 aggcgagttt gtcagtattg tcgggccatc gggctgtgga aaaagtactt tcctcaatat
    11341 cgtcttgggt ctgattgaac cagccagcgg cgtcattgag ttgaacggca cgcgcattag
    11401 tggtccgggc caagagcgcg cgatggtgtt tcaagagttc gggctgctac cctggcgaag
    11461 cgttaccacc aacgtagaac tcggacttga gttgaagggt attccatctg cgaaaagagc
    11521 ggcaagggca acagaactga tcaatttggt cggattgaag ggctttgaaa atcattatcc
    11581 gcatgagctt tccggtggta tgaagcagcg cgtcggcctt gctcgcgcgc tggcaaccga
    11641 gccggaagta ctgcttatgg atgagccctt tgccgcgctc gatgcgcaga cgcgcgactt
    11701 gatgcaaacg gaactactgc agatctggga acgttcaaaa aaaaccgtgc tcttcgtcac
    11761 ccacagcatc gaagaggctg cttatctttc agaccgagtc atcgttatga cggcgagacc
    11821 gggccggacc aaagacctca tccggatcga tttgccccgc ccgagagatt atgaaatgcg
    11881 gcttacgccc aaattcaatg acatcaagct tcgcatctgg gaagtcttga gagaagagct
    11941 gacctctcga ggttagaaat ggcggcacaa gcttttagcg aacaccgacg actctggttg
    12001 ggactcggct ccattttatt catgatcgta tcctgggaat ttgttctgac atatatcttc
    12061 ccggtagatc ccttcttctt taccaaacct tcactgattg gcgcggcgtt taaagagcaa
    12121 gtgcaaggcg gcaaattgtg gcacgatctg gcggtaagca gccaggcttt cctgtggggc
    12181 ttcacgttcg ccgtgatcgt cggcattcct gttggtctcg taatggggtg gcgaaaacgg
    12241 gtcgagtact cactcgatcc gttcctgacg gcgctttacg cgagccctct ggttgccctg
    12301 gcgccattgt tcattatcgt ttttggcgtt ggcgtgctcg gcaaggcagc gctggttttc
    12361 ttgcttgcga tttttccatt catcttcaac acatttgccg gagtaaagtc gactgacgcg
    12421 ctattaatta atgtcattcg ctcgttcggc gggactgaga aggatctcta cttgaaagtc
    12481 attttgccga gcacgatgcc ttacattatc gccggctcgc gcattgcaat tggccgaggg
    12541 ttggttggaa tcattgtcgg tgaattctat gccgcttccg aggggatcgg atttgcgatc
    12601 tcccaagccg gcgataccta ccgtctgccg gacatgttcg tcggcatcat cattctgtcg
    12661 ctaattgcga tcattctgac cgagctgatg cgccgcctcg agctcacgct tgcgccgtgg
    12721 cgcgcaaccg aagagggacg ctgaatgaaa gctaaaaaga tctttgcaga gcacgaatct
    12781 ctgatattcg ggaccgggtt tatcgtgcta ttacttgtcc tttgggaaag cgttccgctc
    12841 tggtacaccc tgccgaaagg gatggcgctc tttttcacca cgccgtcacg aatcgcgtcg
    12901 gcgttttacc aattacttct cgatggcacc attgaaaaac acttttacgt cagcgcagtt
    12961 gccttcctcg cgggactagg tttatccatc atcgtcggat tgccgttagg actcctcatg
    13021 ggcaggtcag tgactctcga gcagttgttg gatccctatg tgacggcagc caatgccacg
    13081 ccgcggatcg tcttcttgcc attattgatc ctctggttcg gcatcggcat ttggtccaag
    13141 atcttgattg tttttatcgg cgcactgttt ccattgctca ttaacaccta tgtcggcgtc
    13201 aagaacgtca accgagttct agtcaatgtc gtgagatcgt ttggcgctaa tgaatggcaa
    13261 gtgatgaaaa tcgtggtgct gcccaattcg gtcccgtaca ttatagccgg gctgagattg
    13321 gccataggca gagcgattct cggagtggtt gtcggcgaat tttttggctc tagccatggc
    13381 ttgggttata tgatcgcgtc cgccgcgacc aactacaaag tcgatgttgt cttcgtcggt
    13441 gttgcaatct ttatggcgat gtcggtgctc ttaaccttag ctatcaaaca gctggaatcc
    13501 cgattggccg tgtggcgccc tgaagcggca aagagcttct agagctagcg ctcagatttg
    13561 ggctttaaaa tggatcattt tcctcccggc ttgcgtccca tctcctttag gacttcattg
    13621 acgatgctac gatcagtgaa ctgattcaga tccggcaacg gttttccctg cggccagccg
    13681 agttcaggag tggttcggga ttcaaaaaga tcctggatca aatcgggcgg catctcgggg
    13741 ctgtacttgt catagacctg agcgtaaatg ctctcgacca ctttgggatc cttgatctcg
    13801 aaatattttt caatcacctc catggtcccc ttcttattgg tccggatgta atcctctgct
    13861 ttgataatac ctcgggtgaa gcgcttgacg acgtcgcgct tttctttgat gacgtctcga
    13921 tgggtaagga atgtccctcc cagacgcggg atatagtcgc cggttcgcat aagaatcttc
    13981 atgccctctt tctccgcacg cgccgcatga ggaaatgaca gcacaccgcc ctgaattgag
    14041 ccctgaatca gcgccgcaat gcggtcgggt tctcccgtaa agctgatcac tcggtaatct
    14101 tttccgggct gcattttgaa gtgtttcccg agtgtgatca caatctcgct gtaatcagcg
    14161 gaaccagggc gacccaggcc caaggttttg ccgcgaagat cctcgacccg cttgatgtcc
    14221 ggcgtcgtcg tcaacgtcca ctgcgaaatt agagattgcc ccacaaagaa aaccagtggc
    14281 gcacctttga gcatcgcttc cgaaccgccg ccgggaatcg gcgtgaactc gatctctttg
    14341 gcgatggtcg ctctgaccag cacacccgca gtcgcagcaa taaaactcgc atccaaaccc
    14401 tcggctttaa aaagcccctt atcaacgcca acgtaggatg gaatgtgaaa accacttccc
    14461 ccgacggtcc tgccgatttt gacggattgc gcagatgcgt ggatcgcgag catcagtgtt
    14521 agcaatgaaa ctgcggcgat ctcgctgacg atccactgac tcgacttttt catgaacatc
    14581 tcctctccgg aaccaaaata accgcgagcg agcctagcat agtccgagcg ctggccgata
    14641 cgcaagacgg ttgctatttg acagccctct tcctccaact taaggtaggg cttccaagca
    14701 gtaggaggaa aaatgaatat tctcatcacc ggcgcaggac tcattggcgc ccacgcggcc
    14761 cgccgtgccg tcgatgccgg ccataaaatt gttctgtacg atttatcgcc gaaccggaac
    14821 tatattcatg agatcgtcgg caccgatccg gtcgacgtgg tggcggccga tattcgcgac
    14881 ttgccggcgc tgctcagcgc gttagaacaa tttaaaatcg acaccgtggt gcacacagcc
    14941 ggcctgatcg gctcgcgtgt tcaggagaat tcttataccg gcgcaaccaa caatattctt
    15001 ggcaccatca atgtcttgga agcagctcgg ctgcgacaac tccgccgggt ggtctacata
    15061 agcacctttg gcgtttatga tcggtcgaag atcgatgaca gtgtcgttcg tgagagccat
    15121 cccatcgggg gccacaatct gtatgcgacg accaaagtgt gctccgagca cctggttcat
    15181 gcctacgccg ccttgtataa gctcgacacg gtaatccttc ggccgggcgg cgttttcggt
    15241 cgaggtttct acgtcggcgg ttccactgtc ggaaaaatca tgcgcgatct cgcgctcaac
    15301 atcatcaagg gtgatcctat tatcctcgat gccaacgcct atggactcaa cgagtatacc
    15361 tatggcaaag atgtcggttt ggcggttttc ctcgcctgcc agaaagacaa tctcaagcag
    15421 cgaatctaca acgccggcac cggcatagtg accggacctg aagagctcgc ccagattgtc
    15481 agagaattat cacctaacgt caaagtcact gtttcgggcg ccgtcaaagc cgataggaac
    15541 aaggcaattc cgctggatct tagtgtgtcg aaggcggagc tgggttatgc gccgcgcttc
    15601 ccattgaagg aagcgttacg ggactatatg gatgaattat ggaaggaggg ttcgcgcgga
    15661 taagctagct gctagtccgc tcagctcatc aatctgaagc gcaacatgta atataaagcc
    15721 gaaacgccgt ctttccaggt aatcttcttg ccctcttggt aggtccgacc ataataggag
    15781 ataggcactt catagaagcg atagcctttc ttggctaact tcagagtgac ttcgggctcg
    15841 aatccgaagc gcttcgattc caatttgata cttctcagga cttcaccccg aaacattttg
    15901 taacccactt ccatatccga aagattcaaa ttggtcagca tgttcgagat cagcgtgagc
    15961 atccgatttc ccagcgagtg ccagaagaat aaaacgcggt gcgcgccgcc tccggcgaat
    16021 cgcgttccat aaaccacgtc ggcaagacct cgctcgatgg gttccaacag cgcgggataa
    16081 tcgtgtggat catattcgag atcggcgtcc tgaatgatga tgacgtcgcc ccgggcgtga
    16141 tccagggcaa ctttaatcgc cgcgcctttt ccctgattat ttggctgcaa aaaaactcta
    16201 atctcgttca gcgggtccgg cgcttgctca ccctctttct gcagttgcgt caaaatttcc
    16261 cggcttcgat ctttcgagcc gtcatcgacc aggataattt ccttgggtaa ctcgatagaa
    16321 cgtactctgc gtacgacctc ttccagcgtg cgctcttcgt tatagaccgg gattaaaact
    16381 gacagcttca ctgctcgaat tcgcgttttt taagctcgcg gatatacgcg gagagcttgg
    16441 ggtcggcgct gacggcttcg ttgagtttag cttggaaatc ggcggcggct cgttcaagag
    16501 ggctcatatc gagccgaatg ccgagccatt gggttagacg cagcaatagc gccaatgtac
    16561 ctcgtggatt gggcgacacc gaaagatagt ggggcaacgc agcccagaga cttaaaccgg
    16621 gtatcgcagc cttacggcat acttcgttta agacaccaat gatgccggtg ggtccttgat
    16681 agcgtgacgg taccaagtcg agttgttcca tcagcttcgg atcggtagag aaaccggaaa
    16741 gcggcaccgg acgcgtgtat agaacctcgt cgagatacgc acccaaagtg accagcattc
    16801 caatttgaca ttcgcggatg aagcgcagca tcgtgtccgt aaaggctcgc catcgtaact
    16861 gcggctccat gccaacccca aacacgaaat ccgcctgagc gccaatgccc tcgccataaa
    16921 agaaatcgta tgaaggccag tgaatttcac gttccttgtt atcgttcaat cgaaccatcg
    16981 gacgctgcac agagaagtca taaaactctt ccgggtcgat gctggcgaat cttttcgcta
    17041 ggagttgatc gcacatatag cgagaggccg ttgtcgccgc cgagccagca tcactccaac
    17101 cggcaaaaga taaaatgaga atgggattct ttagattggg acgactctca aaagtgagag
    17161 gttcaatgac catgaccgat catagcacag tggggctatt ttatacaaaa ttgttatagg
    17221 gcgtctgggc caatgttctg ccgcaggggt ttgagcgcat tggtgaaatc gatgatcaca
    17281 cagcgatggc ggcctttcta cgtcaactct gggaatctaa tccgttgatc gccatcacgg
    17341 tgatccaatc acgcgcagcg accttgtcca attctctgca tccaagccgt cgagactgga
    17401 agccgatcgt caaaagctga tatcatcagt gtgcaacaat ggagagcaaa gctgccttaa
    17461 tcaccggcgg tgcccgcggc atcggtcgcg cagtggccct ggatttggcg tcccgcggct
    17521 ggtccgtggc catttgctac cgcaccagcg caggcgcggc gcatgaagtg gttgacgccg
    17581 caaggcgcca cggcacagcc gcacttgcgt ttcaatgcga tgtctcggac ccaagcgcgg
    17641 ccaccgcgat ggtccatgat ctcggaaacc tatgggggcg cattgacgcg ctgataaacg
    17701 gcgccggtcc ttatcatcgc attcctttgt tggaggaaac caacgagggt tggcattcga
    17761 tgttcgacaa caaccttcat ccggtttttt atttgagcaa ggcagtggtt ccattgatga
    17821 agcaacgcaa ttgggggcgc atcatttgct tcagcatgtc caatgcagag caactgatcg
    17881 ctcagccgca actgaccgca cactacatct ctaaggtcgg agtcatcgtc ctgatgcgct
    17941 cgctggctcg cctggttgcg ccgtatggca tcacggcaaa ctgcatcgcg ccgggttaca
    18001 tagattccgg cagcgcgtcc aaggaggagc tgcaagccgc agtcaaacgc attccggccg
    18061 gctatatcgg gacggtggaa gagacagcgg ctgtggtgcg tttcttactt tctgacgaag
    18121 ctcgctatat caatggcgcc aatattcaga taagcggcgc ctggggagtt taaatctatg
    18181 acttgctctg aagttcatac caaagcattg gagctcgtgc agcaggcaat gaaccgccaa
    18241 atggcgggag agatcgatga ttctatccgg ctctaccggg aatccatcgc gcttcatccg
    18301 accgcggatg cgcatacgta cctgggctgg gcctatagct tccaagggcg aattgacgaa
    18361 gctattgcac aatgcgaaat cgccattcag ctcgatcccc agttcggcaa tccttacaac
    18421 gatctcggtg tttatttgat gcaacagcaa aagtttgatg aagcaattcc ctggttagaa
    18481 cgagccaagc aagcgcaacg ttatgagccg cgccactttc cgtacatcaa tctcggtcga
    18541 gtttatatga ctaaagggat gctccagaag gctatcgagg aattccgcgg cgcggtgaga
    18601 ctcaatccgg cggatacgga gcttgtccag ttactggaag aactcgaaac caagttacag
    18661 tgatttaaag cgctactttg agccgggtcg cgggaacctg agcggagctc caacctctgc
    18721 agcggggatg aagtcgccgc agcgcgcatt catgcatggc tccaggagtc tcgccgtatc
    18781 atcggcctgc gctacgcgag ctcgctaatc gccaggtcga agatttcagc tgcggcggtg
    18841 ctctcttcgc gggtaacgat cagcgggggg caaaagcgca gagtgctctc gccgcaacca
    18901 agcaggagca gacctctttc gaagcaattc ttcatcacct tatcgcgaag cgcggcgtcg
    18961 ggggtacgat ttccgtccgc acgggcgaat tccacgccga tcatcagccc caaaccacgc
    19021 acatcgtcga taacggcgtg tttgttcttt agctgaatga gtttctccat caaataggcc
    19081 ccgacgtctg tggcattatg gagaagtcct gcttcgatca cagctatgct cgcgagcgcc
    19141 gcggcgcatg ccactggatt accgccgaag gtggaaccat gagtgcccgg tgtccacgtg
    19201 ctgatctcgc tgcgagcgat catagcgccc agcggcatgc cgctcgcaat gcccttgccc
    19261 acgcagagga tatcgggctc aatgttccaa tgttcacacg caaacatctt tccagttcga
    19321 ccgaagcccg actgaatctc atcagcgacg agtaagatac cgtgacggcg gcagagatct
    19381 tgaagcatgc cgaggtactc gggaggcggg acgatatacc ctccctcgcc ttgaatcggc
    19441 tcgacaaaaa tcgcggcgac ttcctgtggt gtcacttcgt accggaatag gacttgttcc
    19501 aaataactga cgcatccgac gtcacaggaa ccataattga gatgataggg acagcgatgg
    19561 cagaaggcgt aaggagcatg atgaactccg ggcagcagtg gagaaaaccg cgcccggtgc
    19621 gatgagcgac tggcggtcaa tgatagggca cccatcgtgc ggccgtgaaa agcgccatga
    19681 aaggcgatca cgtgctgcct tttggtggcg tagcgcgcca gtttgagcgc ggcttccacc
    19741 gtttcggtac ctgaattggt gaaaaaaatt tttttggtcc cggttccagg cgccagctcg
    19801 ttgagtttct cggctagctc catcatcggt tggtaataga agtcactgcc acagatatgc
    19861 aaaaatttgc gcgcctgctc ttcgatggct tgaaccacgc gcgggtgaga atggccggta
    19921 ttggcaacgg caatgccggc cataaaatcg agaaaaagat tctcatcgac atcttcgacc
    19981 accatccctc gaccttgctt cacgaccagc ggataaaccc gcccataagc cggcgcagta
    20041 tactggcgat ccttatcgat aattgcctgc gcgttgggtc cgggaagttt tgtcctgatc
    20101 gaaggtacct tcatggagtt ctccgtggct cttgcaggtt gaatagcaaa caatcttgag
    20161 ccggacgaaa gtcattccgc attatcgatc tgcgccttct gcaatctgcc cgagtaatcg
    20221 acataaatag atttccattc actgaagatg tcatagacgg tggtgccggc ttcacgatgg
    20281 ccgttgccgg tatctttgac gccgccgaac ggcagatgca cttccgcgcc gatcgtgggc
    20341 ccattgacgt atgtgattcc tgcctcgata tcgcgcatgg cgcggaaggc gcggttaacg
    20401 tcagcggtgt aaatggaact ggagagcccg taccgcgtgt cgtttagcac ggcgatggcc
    20461 gcatcaaaat ccggtacttc gataactgaa agcaccggtc cgaatatttc ttcttgagcc
    20521 acacgcatac tcggcgtgac tcgatccaat atcgtcggct ggaagaagaa accgttggcg
    20581 catgcgccgg tatcatagct cgaaccgccg atcagcacgc gcgccccttc ttgccggcca
    20641 atttccatat aggcttgcac cttgtcgcgc gccgattgat tgatcagtgg cccgatttcg
    20701 acgctttcat ccaaaccgtc gccgatcttg atcttttcga ttcgcgccac cagcctatcg
    20761 atgaaatcag tcttgatagt acgatgcaga ataagccggc tggtggcggt gcagcgctgt
    20821 ccggtcgtgc cgaacgcgcc ccagagcgca ccgtcgacgg caagggccag gtcggcatcc
    20881 tccataacga tctgagcatt ttttccgccg agctcaaggg agacacgctt gagcgactgc
    20941 ccgcaggcag cggcaacttc tcggccaacc gccgcggaac cggtgaagga aaccaactgc
    21001 acgtcccgat gacgcaccag cgcggcgccc acttcctccc cacgcccctg aaccaggtta
    21061 accacgccgg gaggcacgcc ggcacgttcc aggatttcaa agagcttaac cgcggtatag
    21121 ggggtatctt cagcaggctt gagcaccacc gtgttgccac aaatcagcgc cggaaacatt
    21181 ttccatgttg gaatcgccat gggaaaattc cacggcgtga tcaacgcgca aacaccgact
    21241 ggcgcgcgca ccgacatggc gaacttatcc ggcagttccg acggcgtcgt ttcaccgaaa
    21301 agccgccggc cctcacccgc cacataaaaa gcagtatcga tgccttcctg cacatcgccg
    21361 cgggtctctt tcaagacctt gcccatctcg cgagtcatga tccgagcaat ctcttctttg
    21421 tgcgccctta agagttcacc gactcgatag agcaattccc cgcgtttggg cgcgggcaca
    21481 agacgccacc cagcaaaagc cttcttcgcc gccgcgaccg cggtatcgac atcattctga
    21541 tccgaggctg gaaaaagacc gaccagatcg ttgcggttgg ccggattgcg gttttcgata
    21601 atctctccgg cttgcgccgg cagccatttg ccatcgatat agtttttaaa tacttcagac
    21661 ataacgaagc tccgcgtgtt cgtttctctg acttcagata ccaagcctca tcactgatga
    21721 cgaagcaaaa tcattctaga gccggcccag cgtgaacaca aacgttactg atccgtgagg
    21781 atgtctaaac tcgtgtaatt cgttgatttt tgacaatcaa tcagttagct tctggcaact
    21841 cgccggtgga gactatcgga tcgagcggcc aaaactagcg aggagaagga gatctctatg
    21901 cggcaacttt cttgtttgtt cacggcagca gtcatcggat tgtttgcgct ccgacccgcc
    21961 gccgcggcag aagactttta taagggcaaa accatcagaa tcgtcgtcgg gttttccgcc
    22021 ggcggaggat tcgacactta cgcccgcgcc atcgcccggc acatgggcaa acatattcct
    22081 ggggaaccgg caatcatcgt ggaaaacatg actggcgccg gcagcctgat tgcagcgaat
    22141 cacgtctaca aggttgccaa accggatggc ctcaccatcg gccacttcat cggcggctta
    22201 ttcttaggtc aggtgttggg gcaaaagggc atagaatttg atgcccgcaa attcgaattc
    22261 atcggcgcgc ccattaccga tcacgtcgtg tgcgcgctaa ccaaagccag cggcatcacc
    22321 agcgtggaag catggatggc atccaaaacc ccgataaaaa tgggtgggat tgcgcccggc
    22381 acgtcgactc ccgacaacgc gacacggatc ttgaaggaag cgcttggact gccgattcaa
    22441 atcgtatcgg gctacaaagg cacggccgat atcagattgg ctgccgaaag cggcgagatc
    22501 gccggcggct gctggggctg ggattcggtc agcgtgactt ggcagaaagc actcgccagt
    22561 ggcgatgccg tggttgtcct ccaggcaaac cgccgaaatc accccgacct tcctaaagtc
    22621 ccccaagcaa tcaagctggc caaaacagaa gagggtcgca aaatgatcga agtgggaatt
    22681 cacggcgata gcgatatcgt tcgcacttac acgctaccgc cgggaactcc gaaggatcga
    22741 gttcaattgc tgcgcaaagc attcgatgag acgctaaaag atcctgaatt cctggcagat
    22801 gcaaagaagt cgaagttgaa cgtcgatcct gtggacgtcg aagaagtgga gaaagtgatc
    22861 gccggcctct tcaagctcga tcagtcgctg atcagcaaac tgaaagagat tctttacaac
    22921 tgaaaactcg ggattgaccg tcatgttaaa tcgctgtatc gcgtcgaggt agtccctcat
    22981 ggtggaaatg ttcagcgcgc tgagttccgg actggttcaa gttttcaact ggtctacctt
    23041 cagtttgatg atgatcggta tcgccgtggg cttcgtcgtc gggattcttc ccggcttggg
    23101 cgggcccacc gcgatggctt tgatgttacc cttcgttttc aaaatgaacg cagtcgaagc
    23161 gttcgccttt cttctgggga tgaccgcggt tacggccacc actggagata tcacgtctgt
    23221 cctgtttggt gttccgggcg aaccgacgac cgcttcgacg attgtcgacg gtcactcaat
    23281 ggcgcgcaac ggtgaagccg gccgcgcgct cggcgccgtg ctgatgagtt cgctggtcgg
    23341 cgccattttc gccgccgtcg ctctcgcgat cgctatcccg atcatccgtc ctctggttct
    23401 ctcgttcggt tctcccgaat ttttcatgct ggcgctcctc ggcatctcgt tcgtcgcttc
    23461 tctgagcggc gaagaccagc tcaaaggaat cgtcgccggc ggcatcggcc tattgctcgc
    23521 caccatcggc ctggatccga tctccggaat ccaacgctat accttcgggc agttgtttct
    23581 ttgggacggt atcggtctgg tgccgatcac gatcggcttt tacgccattc ctgaaaccat
    23641 tgagcttgct gtcttgggga ccagtattgc taagcaggaa gtaggaaaac tcggcggcgt
    23701 catggaagga gtgaaagata cctttcgtca cttttggctg gtaatccgct gcagcgcgct
    23761 gggctgtttt atcgcaatca ttccaggcat gggagctgcg accacgcagt ggcttgccta
    23821 cgcccacgcc gtgcagagct cgcccaacaa agaacgcttc ggcaagggcg atgttcgcgg
    23881 tgttttgggt cccggggcgg ccaataattc aaccttgggc ggaagtttga ttacgacgat
    23941 tgctttcggt gttcccgcca gcgttaccat ggcgatcttg ctcggagcat ttatcattca
    24001 gggaatcgtc cccggtccgg acatgctgat tcctccgccc aaaggtaaac tcgaccttac
    24061 cttttcgttc gtatgggtga tcattctttc caacatcatc acggttgcga tctgctttct
    24121 gttcttgaaa ccgctcgcca aggtgactca ggtacgtggc ggtattatca ttccgctgat
    24181 cttggtgctg atctacctgg gcgcttacgc cgaaaagaac gcatttgccg acatggtggt
    24241 ggtgcttctg ttcggcgggc tgggttggat catggagaag atggagtggc cgcgcccgcc
    24301 ggtgttgctc ggtttggtgt tgggtccttt agcagagaat cgcctctttc tctccaccga
    24361 taactatggc atgaattg
//
LOCUS       DASUVS010000179        28507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_728410, whole genome shotgun sequence.
ACCESSION   DASUVS010000179 DASUVS010000000
VERSION     DASUVS010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 28507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28507
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728410"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VF182_09915"
     CDS             complement(<1..573)
                     /locus_tag="VF182_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6437435.1"
                     /translation="MVRRLSLDGGNLALALVIAILIYQVVIPLLMVVWTSLKVERPGE
                     VGFFELSFSLTNYARAFGSAYFWQATWNTLYFAVASTIVSFGFGTFLAWVVHRTNTPL
                     ARLIGVITLGRIIIPGILITVSWILLASPSIGVLNHALEGATGIKGILNVYSFWGMVW
                     VHSLEMTPLAYLLLSAALQSMDPRLEEASGA"
     gene            complement(619..1695)
                     /locus_tag="VF182_09920"
     CDS             complement(619..1695)
                     /locus_tag="VF182_09920"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6437436.1"
                     /translation="MKRFVNSLMACAVLAMSAVDSLYAAATQSPEQLFAELAKLKPDQ
                     RLKRLEEGSRKEGKLNFIHTFRGKLAREHVRLFEKRYPFVKVEMADMGSQDAAERFIA
                     EETAGRHLTDTISLAVPDLPVILKQDLLADYPTPATSRIPKQFEGFLDKQNRWVPYYW
                     SEHGISYNTNLIPPDKAPKSWQDLCNPAYKGQVSFDPPETRFMVGLYVLMGEEKFQEW
                     LKCMGQNQPIIQRGHTQRMNLMLSGDHALQGDNFLYVGAEEKKKDPKTPYAIVYSAPI
                     LAYAGVMIINKNTRSPHAAALFSDWTLSEESQKYIAAEFRGPLIGKHPYLPDNIKVVT
                     FGYVSDDIVDRLHGYWNQYIGKKK"
     gene            complement(1858..3324)
                     /gene="tcuA"
                     /locus_tag="VF182_09925"
     CDS             complement(1858..3324)
                     /gene="tcuA"
                     /locus_tag="VF182_09925"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX6437437.1"
                     /translation="MKHETIPVVVVGGGSAAFEAAVAAKQAGAPRVVMLEKAPEPEFG
                     GNARYSHTGFRWVFSGAEEVRRFLPDLDEQTFNKLHLPAYSAEQFTADLQRVTRGRID
                     TELADVMVQQSNAAMHWMRELGIKWEIDSHVMIEGRYYFEPGLVIHPVGGVGGGLGQL
                     MQWREIAMRMGIEIRYESRVKQLLGNDRRIEGVAVSDAKEEYDIRTGSLILCAGGFQA
                     NPEMRARYLGANADLMKVRGSRHDTGEVLMMALDLGCKASGHWQGAHATPIDSTFPDV
                     EIGSKANRYGYPYSITVNSLGQRFFDEGEARHSYTYAKTGWAVLGQPGAIAYQIYDQK
                     TIPLLGTRYEFSTPIVASTIHELAGKIGVEPAVLDHTVKEFNNSARGDIAFDPTKLDG
                     KCTEGITPKKSNWASPVDQPPYWAYSITGGITFTFGGLQINESAQALNTSGNPIRGLY
                     ASGDIVGLFYHNYPSCTGQTRNVVFSRLAGRHAAAQGL"
     gene            complement(3392..3790)
                     /locus_tag="VF182_09930"
     CDS             complement(3392..3790)
                     /locus_tag="VF182_09930"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6437438.1"
                     /translation="MPKLKHLAIRTEDTGKLATFYMDVFEMQVLHKDKNEGGAIFLTD
                     GYFNLAILPNHEQQAPNGLYHFGFEVESGEKIVERLKKVNPNKLPKPRPNGRPYAETR
                     GSDPDGNYFDISEHGFSNAVPLGENKDRAN"
     gene            complement(3845..5314)
                     /gene="tcuA"
                     /locus_tag="VF182_09935"
     CDS             complement(3845..5314)
                     /gene="tcuA"
                     /locus_tag="VF182_09935"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX6437439.1"
                     /translation="MTIKHASVVVVGGGSAAFEAAVAAKQAGAPSVIMLEKAPEAEFG
                     GNARFSHTGFRFVYSGADEIRRFLPDMDEASFSHLQLPGYHPEHFTADLLRVTRGRIN
                     KDLAEVLVNESNGAIHWMRELGIQWEPAGSVEIDGKRYFEPGIVIHPTGGVGGGVSQL
                     NQWRAIASRMGIEIYFDSRVRELLGNDRHIEGVRVSDFNEEYELRAGAVILCSGGFQA
                     NPEMRARYLGANADLMKVRGSRHDTGEVLQMALGLGCKASGHWQGAHATPIDATFPDV
                     ELSNKANRYGYTFGITVNSLGQRFFDEGEAHHSYTYAKTGWAVLGEPGGVAYQIYDRK
                     GVAFCDKSYYAFATPVEASTIAELASKIGIDAAILRHTVDEFNRAVRGEMPFDATRAD
                     GRCTEGITPRKSNWALRIDEPPFHAYPVTGGITFTFGGLEVNRQAQVLNTGGRPIRGL
                     FASGDILGLFFHNYPSCTGQTRNVVFSRLAGRHAAAQGL"
     gene            complement(5356..6363)
                     /locus_tag="VF182_09940"
     CDS             complement(5356..6363)
                     /locus_tag="VF182_09940"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437440.1"
                     /translation="MATYHRKFLCVFALAVGFLPTQSPAQIKSNLSSSITSESMAAVW
                     IARDRGLFKKYGLDVQYILMPRSPLAVSALVGGEIDMAVIGPGHLVNAAAGGTDVIGI
                     ANFAQKLDYRLNTRPEIKKKEELRGKRIAISGPGSTSHLVSLLALQHLALDPNAAKIA
                     FFTISGTEINRRLALESGSIDATTLNGSVGDLYGQKGYPVMFNFKGSGVTMPQTMLVT
                     TRRLAITKPQVVDAYAKTIVEAIAVMFDPANKESVTRTIASNLRLSNPSDSEQAYQSV
                     INSYERVPYPSPEGMQRLHGLLTTINPKLAGVKIDTVVDDSFIRKLDSSGFVESVSKK
                     H"
     gene            complement(6396..6788)
                     /locus_tag="VF182_09945"
     CDS             complement(6396..6788)
                     /locus_tag="VF182_09945"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6437441.1"
                     /translation="MPKLRHLAIRTEDTGKLATFYMDVFEMQVLHKDKNEGGAIYLTD
                     GYFNLAILPNHEQQSPNGLYHFGFEVENGEKIVERLQKFNPNKLPKQRPNGRPYAETR
                     TSDIDGNYFDISEHGFLTVEPVGEKKPN"
     gene            complement(7080..8474)
                     /locus_tag="VF182_09950"
     CDS             complement(7080..8474)
                     /locus_tag="VF182_09950"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX6437442.1"
                     /translation="MQYLAICEDDLLFQFMRDVATEHGEVRFLVQEETVARRIKRMGG
                     KAWYGDLLKEETYKKVKLHHIDQAVVFLRDPESQDRLCKLLRTMDKALPVVLLTNDKN
                     CKKDENDPLVRQIQLTHTFREFWAAHLLDTLNRKKVESIRALFGEEKVHILLQHDPDP
                     DAIGSALALRELLGRNRATTPIVTFGGVARPENLAMIRLLDVQIDRITHDDLHKEGAK
                     LAMVDVQPPYFDKPLGKVDLVVDHHPKRANFKARFTDLRTPYGATSTIFTEYLRAAGI
                     EPSQRLATALLYGIKTDTLFLERGSNLADLSAFTYLYPFANKAMISRIERPALPREDL
                     EALGRALSRLRLDNGVAVIHMGEINREDVIPQMAEFCLQIEGVEWGVVSGLVNERVVI
                     SVRNVGYVKSAGEIMKKLYDEIGSAGGHRAMAKAVIPLDRFKERFGEVSDRVIRDAML
                     PLIVERETEHSAHG"
     gene            complement(8713..9741)
                     /locus_tag="VF182_09955"
     CDS             complement(8713..9741)
                     /locus_tag="VF182_09955"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6437443.1"
                     /translation="MPELPATQADLSPRSLMRILGDFANSQILDASIEYDFFTLIDNG
                     FHTANEIARQAGTDPRATRIVLDSLLATALVQKRNSLYQLTPVSEAFLVRGKPSYVGD
                     FRHVALALWDGMAHLKESLKTGKPFSRLDTVDALEVWEKLVLGIIVIAEPAARALCDV
                     LKIGTERKAVNVLDIAGGSSIFGMTILSRDPSAKVTQLDWPNVNAVAKKLNKERGLEG
                     KIRFIDGEHHSARIETNHYDLVLASNFCRFESPKRNQELFAKAYSALKPGGYFIVNDF
                     VPNEERTEPTFALRFSVYTLTHTPEGECWTLSQYAEWMDEAGFGALETHGDIPGTLPG
                     TMLILARR"
     gene            complement(9744..10541)
                     /locus_tag="VF182_09960"
     CDS             complement(9744..10541)
                     /locus_tag="VF182_09960"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437444.1"
                     /translation="MPELEGKDYSGKRLSPQEADAAIADLKKYLLELADRWLFKGRFH
                     TDMSAGTLPMEAIKVFWQNWYGFVAEINNFHGVAYQRQLPFFKRHPELQKHFANHVAD
                     EMIHPTPPGHIQIVLEQGRNFGLTHDQMIEYEMLAECRAFLEYYRGVLYEGTMAEWWA
                     SFCVEEAVGHWARFFGSALRQNYHLPEEKVVYFRVHAQADLEEHQGVMAHAELDWTVL
                     RKLLEEGRAETRPGFNLAYCLRMGTAYFAWFFEGVYQHIKKEGLYKT"
     gene            complement(10559..12043)
                     /locus_tag="VF182_09965"
     CDS             complement(10559..12043)
                     /locus_tag="VF182_09965"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6437445.1"
                     /translation="MLSLDEMVVRLKEIAGGRLVSTNPIDKINYQHTLAYGAYRALPD
                     VIVRVESDAQGNHALAKILSFANEHKIPIVAKGGVGMGISSPLKGGILLDMLGMDKIL
                     EVNPSMQYIIAEGGASVYAIHHALRQHGLMLPNFGTYGPAVVIGAMINKGGIGYGMTR
                     YGWIADMVLGLEVILANGETVRLGALANQGTTFGPFQKWIHLPDLLGLFTLAAGSMGM
                     ISKVCLRAIEGKREWLHDYCYTFRRDQLLNAQEALMQLVKSEVVYDIHFTDRWQYHWP
                     MEEGLYHKSRIPDDAWFFLKTVIMARDAEEIAFKEQRMRRIYHNAGGIDVPEIAAKAM
                     GAKAQEHGLHGWPDYHVMGPDGWCGAVARHSGMFAVTSKMYPLTFLKEMYDLDEAAKR
                     ECGIWDAEHSPQHDSYVTRDLAIKEEYFVFYNPYDDEDVGKLRKWMSMVQRFGNERGV
                     VWTAPTLSTKGPLPYERLTNLYDLVKEIKQLVDPNNILNPGALY"
     gene            complement(12149..12922)
                     /locus_tag="VF182_09970"
     CDS             complement(12149..12922)
                     /locus_tag="VF182_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6437446.1"
                     /translation="MTEQDKHLQLREKVVLVTGASRGIGAAAAKRLAQSGASVIINYL
                     RNREAAKRVLDEIRGAGGEGMVFQADVRRKDQVDLMIKGVREHYRAIDVLVNNAYFPF
                     QVGQLHELSWDDFHRAIEHELIAFHNCVQACLPDMIEKKSGKIVVVSTRLAQQPLPKM
                     GAYAAAKSALESMADTMAIELGPVGINVNVVTPAFTLTEASSIMPDAYRERVRENRPL
                     KKHLYPEDVAGAIAFLAGDQASMLTGSHVLITGGSHLQL"
     gene            complement(12937..14418)
                     /locus_tag="VF182_09975"
     CDS             complement(12937..14418)
                     /locus_tag="VF182_09975"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6437447.1"
                     /translation="MKILFVTPPMGNWAPWGDRHVAVNSLHAQLAGFVREHNATEVEV
                     LDCRALALDDDGMLEKIKQTRPDVAFFGNMIAAAGGAAQLNRFHAAMSRIKDILPDTI
                     TVGGGLMYTAVPQKIMADNPQLDFAIVGVFGDNEHTLCELLEALKMPSPRFATIKGLA
                     YRENGEVVLNPPRPLISNLDDLPLPAYDLFPMDRYCGYSVIPNYNETVTSRGCEGACH
                     FCYEWWLVDQRNPRDFSSHRTKGGKKVVEEMELLNQKYGVKALTFMDDDFNSDRQKMV
                     DMLEELEKKRLDMSWFMLSRAQNLIRDADLIPRLRKVGLYQVLIGIDGGTDEEIAEAR
                     KGPMKVGVQELKELIKFLRKNDISTISTYLNGFWEDDEAKIRQRAKAVDEIDPDIVMI
                     QLLNPIPGSPIYKKAVKENLIEIDNLSLYDLEHCVMPTKYLSRQQLGELTGWAFQSFY
                     SKPGRIERILNGYTSPYVKMKFLSFKNNAAKYEKGASEDAVAI"
     gene            14644..15489
                     /locus_tag="VF182_09980"
     CDS             14644..15489
                     /locus_tag="VF182_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6437448.1"
                     /translation="MADQAAEEQLLDVSVAEASAAYKFYLNNEKKILGTSAVVLFLTA
                     WELVGNVLGMINPMFMSAPSLIFKAAIQMFSSGEIYNDLYVSGLEFAWGYLLSVAVGV
                     PFGIACGWYKKFAYIFDPFINAMNATPRVALLPLVIIWLGIGILSKVGIIFLGAVFPI
                     LINARDGVKTTPYNLLTAARSFGASEWQIFKSVVLPSTLPFILTGLRLGVGRALIGVM
                     VGELYAATAGIGFMITVAGATFQTDKVFVGVLIFAISGMIAMELLTRLESRFDKWRPK
                     VGAVA"
     gene            15666..18353
                     /locus_tag="VF182_09985"
     CDS             15666..18353
                     /locus_tag="VF182_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX6437449.1"
                     /translation="MKGLGGLFLFAIFLVLISIGQLISLYVDWLWFHEVGYTQVFTTS
                     LFFKTVLALVGGGIFALLIYANVRIAARLVRGIRFLDQENFLDLPRPELIDPLVQRLL
                     LPAALVLGLFAAPQAAKHWESFLLLLNPTSFGLEDPLFNKDLSFFVFQLPALRTLYNW
                     LMAALALSLALTAIVYVLYRSLQYSPRGIFLAPRARLHLLILIAALFAVKAAGYVLDT
                     YDLLYASGGAAFGAGYTDVYVRLPALRILTVAALIAAALSVAQITRPGFGYLLVGIAG
                     LVVVHVIGLSAAPAMVQHFRVVPNEVDSERPFIEHNIKFTRTAYGLDKIENEDFPADE
                     TLNAADLQRNDATIKNIRLWEHQPLLATYAQLQEIRTYYKFVDVDNDRYNIDGTYRQI
                     MLSARELSHQHLPSRIWINEHLTYTHGYGVVFGPVNQVTPEGLPEFFIKDIPPVSEGS
                     IKVTRPEIYYGELANDYVFVKSGARELDYPSGDQNIYTQYSGSGGVSIGSLWRKLIFS
                     AHFATLRIVLSEDIVQESRILYQRQIQERVKKILPFVTFDRDAYMVIAQGGRLFWMID
                     GYTTSDRFPYSAPIRRRGTNYIRNSVKAVVDAYNGTVDFYMSDPDDPIVQSYQKIFPQ
                     LFKPLEKMPEDLRAHIRYPEDLFTIQAQMYATYHMRDPQVFYNKEDLLSIPRQTTSGS
                     EREMAPYYTIMRLPGETKEEFVLLLPFTPNNRDNMRAWLAARSDPPHYGKLIALDFPK
                     AKLVYGPKQIDARIEQDAFISQQLSLWGQRGQVIRGSLLAIPIEKSLLYVQPLFLAAE
                     KGSLPELKRVIVAFGNQIAMEETLDQSLQRVFGGKASTEPTPVAASVKEARIDSTLAQ
                     RALQHFSRSQEFLRQGNWAGYGEEQKRLEAILRDMQQGR"
     gene            18489..19298
                     /locus_tag="VF182_09990"
     CDS             18489..19298
                     /locus_tag="VF182_09990"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX6437450.1"
                     /translation="MKQMHPSWDCSNSLDLVSYEFYCYKAAMTGQQRQLAIRGLIFVV
                     LIGLLFLFSRYLPQPFSALVPPGEEIDDFEGIYSSSDKLGNFLRSLGPYSPAVFVLFQ
                     VSQVIAAPFPGELTGVAGGYVYGETLGFILSTIGLTLGSWVAFELASILGRPFVERFV
                     KQDVLHRFDFLTTNTGAAICFLLFLFPGFPKDYLCYVLGLSRMKLATFLFVTIVGRIP
                     GTYFLTMQGASIRNQEYYTAIIFAVVSAVILLIAYLYRGRIFSWMKHRHEI"
     gene            complement(19357..20187)
                     /locus_tag="VF182_09995"
     CDS             complement(19357..20187)
                     /locus_tag="VF182_09995"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX6437451.1"
                     /translation="MEGWAVAVILGVVEGLTEFIPVSSTGHLIIASHLLGFFGEKAAI
                     FQVAIQFGAILAVLLLYWRRFTDLLPVSNNSTRFRCEDSNLLGWAGIWRLTAATLPAL
                     VVGFLTKDLIKTHLFTPWAVTVALAGGGLAMLIAERLLKRRTSKPLETVTIAQAIGVG
                     LFQVLALWPGTSRSAATIVGGMLLGLDRKGAAEFSFLIAVPIMLVVECYELMKMGTLF
                     SAQDATNFMIGFLVSFIVALMAVKTFIFFLSKWTLVPFAWYRIIVAPIFFLLTGETRL
                     "
     gene            complement(20242..22020)
                     /locus_tag="VF182_10000"
     CDS             complement(20242..22020)
                     /locus_tag="VF182_10000"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6437452.1"
                     /translation="MSATTQPSINIRLESDIIEPPRRWLPWLLLAVGLTSLFYQLGTA
                     GLFEPDEGRNAEKAREILLLNDWVTPHEDFFPVLDKPMFFYWLIAISYKAFGVNEWAA
                     RLPSVLAALGCFALVYSFARIRWGPWEALWSGLILISGVEFFILSRVVIFDMSLTFCI
                     SLALYSFYQAAHADAAMQRRIYCLLMYGALAAGTLIKGLVGLLIPGLIIFFYLLIANR
                     WSNLGSVYLIPGALLFLALVTPWYLLAEARNSGFIRYYLWDEHFGRFATASFDREEPW
                     YFFLGVLVVGFLPWSALLPSVIRRYWSQSLDDRKIFLIVWSTVPLVLFSVSRSKLPHY
                     ILPIFPALSILTATTLSSMFQNRAHNLKRPLAFFWALQSLSIWYLVLGLIWPVILPDR
                     IRGAFISMKWSIVVYAIICLAVLIALGWGKIAQLWRSQSTVFALHAAGAFFFSVLLAQ
                     IMVSVSRDRSAQEIAGAVAARTYADTQVVLYDTYLTGMPFYLRAQRPVWIVTHANKKK
                     TVLGNFYVATHRAWPDTRWGKALFDLDEFRRVWERNDTPLLIIVKQKNISRMEREIGA
                     SPRKLTSIDEYVLMSNRQLSDNQGHE"
     gene            22192..22407
                     /locus_tag="VF182_10005"
     CDS             22192..22407
                     /locus_tag="VF182_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX6437453.1"
                     /translation="MFGLIRIPELLIVSVIALVVFDPANFPDFRKSLGLSIRGSMKTL
                     TDFVRWAIERSGSSTPVNQAFTFQFVP"
     gene            22427..23650
                     /locus_tag="VF182_10010"
     CDS             22427..23650
                     /locus_tag="VF182_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437454.1"
                     /translation="MKRHPIKVATIILLFLSTFLTPTSNLLYAQQPVASFFYDDQGHV
                     TQQARDTNGDGKMDRWTHYNERGQIERVEQDMNFDGKPDAFVYYENGKPRRQEVANNN
                     DGRINLWYLLDPDGKVERKGEDPNRTGKPTVWVYYQDGQPLRAEEQNGPSGVANRHVY
                     FRDGKISRVEEDTDADGKPDSFAFYDNGQLVRKELDRKHTGKTDLWGYYQDNRLVKQE
                     EDLRGDGTVSRIVYFQNQIVVRREEDTMGRQRMDTIDSFTQGKLTKRLRDTKGTGKWD
                     AVYVFENDQVVREERDTDADGIFDLRIFYADGQIRRQEGDTNGDRRVDVWVIFENGER
                     IEQLEDQHSRGMISARYIFKNGQVVEQQAIADAQPAPMALPFVAAEDELRSMTASTGV
                     QTDERRATAATAGLQ"
     gene            23818..24825
                     /locus_tag="VF182_10015"
     CDS             23818..24825
                     /locus_tag="VF182_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437455.1"
                     /translation="MPQYPTQSQVTSSQRWNHAWNKAMEGAFVGLNIGGPFGGAGGFT
                     LGLLTGLITAESHYGVLNAQIQSEQEKDRQLEAAIEQELQRQSGFENQVTQATLTSAG
                     TPQDKPAALPGEQASVAPTVPPVRTASGNVAVASVNKSPGSIPPTQFKNVEVKDINGD
                     GVPDLWTYHNPRNPGEIVRQEESTKYDGTVDAWSYFKDGKLVRRDVDTRGQGRPDTIF
                     YYADDKIAREERDESGTGRISYRAIYQNGRLAQVEKDTRGEGKIDLWIHYDTNGTEEI
                     IIKDEKDMNGDGIADLWTYYENGRVVRRDVSALGLELLAKQEQISLPSDDVKQIQVPG
                     N"
     gene            24830..25849
                     /locus_tag="VF182_10020"
     CDS             24830..25849
                     /locus_tag="VF182_10020"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX6437456.1"
                     /translation="MNDLASAWEDSVLSLFRSAFLVPSLLLHALLLGLLFRAAGLSVP
                     RPGFDPPISVQLMEVNEGGSRDKSIGSNKGPGGQRSLPKRGTTVAPAQRSGKLDSGSV
                     EESKPSDTVEATPAPKPMPLPGPKLLSSDSTREAVNVKETSPDSLVRLPTKESATNLP
                     GAVAIDSETSQKSLAALKSTDSGAGIKALREAEQIPGSLKGVGTGAGPYGVPGGSRSG
                     TGLSGGGTGSGTGGGSSTGLKGIANSDYNQYLQQLKRRVESVWKYPDDVTGIQKVAVR
                     FILDRAGKLTLAEVLDSSDSRLNTSAVEAIRRASPFPPIPESLKDLANEPMIIRFEVA
                     IRVRG"
     gene            25934..26560
                     /locus_tag="VF182_10025"
     CDS             25934..26560
                     /locus_tag="VF182_10025"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX6437457.1"
                     /translation="MEQQGLNLIEIIHKGALATYPLILLSVISVTVVFERLWSLRNIR
                     STTLRIAESLIDPVQKGQRDLALAICKQNSHCPAGRIFHSVIEGAESHRPETGSNVAV
                     EAMFEETQHLRKHLWILGTVASSAPFIGLFGTVVGIIKAFESMAVSGTGGFAVVAAGI
                     SEALVATALGLAVAIIALVFYNYFQTRIGSLTALFRIQVARVLRHFNP"
     gene            26568..26990
                     /locus_tag="VF182_10030"
     CDS             26568..26990
                     /locus_tag="VF182_10030"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX6437458.1"
                     /translation="MALTPLGSNEENPDIVAEINITPLTDIFLVLLIIFMITSSAMIE
                     SGGKVNLPKAVATQSESRGTTVTLTPKREIFVNQKQISEDKLEPALKEALAGSVDKVV
                     ILRGDKDVLLGDTVKIMSIIKRAGASEIAIAAETEKSR"
     gene            complement(27004..28266)
                     /locus_tag="VF182_10035"
     CDS             complement(27004..28266)
                     /locus_tag="VF182_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6437459.1"
                     /translation="MKLIFLRCAFLATFVLSLLSRPFPLVSAEAKVQIHSPKDGATIN
                     QEQNLVFVSGKVATTAARSANVDIMLLLDASGSTANYAGVDLTGMEQLPGTGSGFTTP
                     QIFIGGMSVGGPAMRNLRNSILAAEVIAARRLLPQLNSQTTRVGVVSFGESAKLVQPL
                     THEFDRVRVALDEVYKAGPYGGTNMVEGIRTGITELMGMGSSEKKTDAVKVEFLLTDG
                     FPTMPIGGGKSATPQDTDLAINAARLAGRAGIKVHVFALGDEALSYPRAAVGIAKQSG
                     GIYTPVSRPVDILSVVENISAVGVDYVQIVNQSSGQKANQLRVAADGFFSAAVPVIEG
                     RNQIDVFARASDGSNGKDSVTVNYQSGNQKSLELEVFLEREKNLKLEVERLGKSQAEI
                     QQDIERARQDGLGRSQRPLPPDQGTQVR"
BASE COUNT         6727 a         7658 c         7492 g         6630 t
ORIGIN      
        1 cgcgcctgac gcctcttcga ggcgcgggtc catggattgc agtgcggcgg agagcagcag
       61 ataggcgagc ggggtcatct ccagcgagtg gacccaaacc attccccaga aagagtacac
      121 gttcagtatt cctttaatcc cggtggctcc ctccagggcg tggttcagaa ccccgatgct
      181 cgggctcgcc aagaggatcc aggatactgt gatcagaatg ccggggatga tgatccggcc
      241 aagggtaatg acaccgatca gccgcgccaa aggagtgttg gtccgatgca ccacccacgc
      301 gagaaaagtt ccgaagccga atgaaacgat agtggaggca acagcgaagt agagggtgtt
      361 ccaggtcgcc tgccaaaagt atgctgaacc gaaggctcga gcataattgg tcagcgagaa
      421 actcagctcg aagaatccca cctctccggg ccgttcgacc ttgagactgg tccagaccac
      481 catcagcagc ggaatgacca cctgataaat caggattgca ataacgaggg cgagcgcgag
      541 attgccgcca tctaacgaaa gccgccgcac catcactgac gagccggtcc catattcaac
      601 cgattgtcga tggcagggtt attttttctt gccgatgtac tggttccaat agccgtgcaa
      661 acggtcgacg atatcgtcgc tgacataacc gaacgtgaca actttaatgt tgtcgggcag
      721 ataaggatgc ttgccgatta gcggcccgcg aaactcagcg gcgatatatt tttggctttc
      781 ttcagaaagc gtccaatcgc tgaatagcgc cgccgcatgc gggctccgag tatttttgtt
      841 gatgatcatc acgccggcat aggccagaat cggcgcggaa taaacgatcg cgtaaggggt
      901 ctttggatct tttttctttt cctccgcgcc gacgtaaagg aagttatcgc cctgaagtgc
      961 atggtcgcca gaaagcatca ggttcattcg ctgcgtgtga ccgcgctgaa tgatcggttg
     1021 attttgcccc atgcacttaa gccattcctg gaatttctcc tctcccatca agacataaag
     1081 tccgaccatg aagcgggtct cgggaggatc gaaggagacc tgacctttgt acgccggatt
     1141 acaaagatct tgccagctct tcggcgcttt gtccggcggt atgagattgg tgttgtagga
     1201 aatgccgtgc tcgctccaat aatacggcac ccagcggttt tgcttatcca aaaacccctc
     1261 aaactgcttc gggattctgc tggtagccgg ggttggataa tcggcgagta ggtcctgttt
     1321 cagaatgacc ggcagatccg gaacggcaag gctaatggtg tcggtgagat ggcgtcctgc
     1381 ggtttcctcg gcaatgaagc gctcggctgc atcttgagac cccatatcgg ccatttccac
     1441 tttgacaaac gggtagcgct tctcgaacag acgcacatgc tcgcgcgcga gcttgcctct
     1501 aaaggtgtgg atgaaattga gcttcccctc cttgcgtgat ccttcttcga ggcgcttgag
     1561 gcgctgatcc ggcttcaact tggcgagttc tgcaaatagc tgttccggag attgggtggc
     1621 ggctgcgtat aatgaatcaa ccgcactcat tgccaacacg gcacatgcca tcagagaatt
     1681 cacgaatcgt ttcatggtaa tcccttttcc tcggaagcag cggtctcgac tgcgtcgcac
     1741 catcagaaaa ctttgatata cgccaggcga tgagcgattt tcaaggtgca agttgcaatg
     1801 attatggctg gaattctgct gagggacaga gagcgtgaga aggaaaatct cggttcatca
     1861 cagcccctgg gctgcggcat ggcgtcccgc caaacgggag aaaacgacgt tacgtgtttg
     1921 tccggtgcag gacggatagt tgtgatagaa cagaccgacg atgtcacccg aggcgtacag
     1981 accgcggatc gggttgcctg aagtattgag cgcctgggcg ctctcgttta tctgcaggcc
     2041 gccgaacgtg aaggtgatgc cgccggtgat cgaataagcc cagtaggggg gctggtcgac
     2101 gggacttgcc caattggatt tcttcggcgt gatgccttcg gtgcacttgc cgtccaattt
     2161 ggtcggatcg aacgcaatgt cgcctcgcgc cgaattgttg aactctttca cggtgtggtc
     2221 gagcaccgcc ggctcaacac cgatcttgcc ggccagttcg tgaatcgtgc ttgcgacaat
     2281 tggcgttgag aattcgtatc tcgtcccgag caacggaatc gttttctgat cgtagatttg
     2341 atacgcgatc gctcctggtt gaccgagcac agcccatccc gtcttggcat aagtatagga
     2401 atggcgcgct tcgccttcat cgaaaaaacg ttgccccaat gaattaacgg taatgctgta
     2461 cggataacca tagcgattgg ccttgctgcc gatctccacg tctggaaaag ttgaatcgat
     2521 gggtgtggcg tgggcgccct gccagtgccc cgaagctttg caccccaagt ccaaggccat
     2581 catcagcacc tcgccggtgt catgccggct gccgcgcact ttcatcagat cggcgttagc
     2641 gcctaggtag cgcgcgcgca tttcagggtt ggcttgaaac ccgccagcgc aaagtatcaa
     2701 ggaccccgtg cggatgtcgt attcttcctt ggcatccgac accgcgacgc cttcgatgcg
     2761 cctgtcgttg ccaagcaact gcttgacccg tgattcatag cggatctcga tgcccatccg
     2821 catcgcgatc tcgcgccact gcatcagttg acccaagccg ccgccgacac cgccgaccgg
     2881 atggatcacc aagccgggct caaaatagta acggccttca atcatcacat ggctgtcgat
     2941 ctcccatttg atcccgagct cgcgcatcca atgcatggca gcgttggatt gttgcaccat
     3001 gacatcggca agctccgtat cgatccgacc tcgagtcacc cgctgcaggt cggcggtgaa
     3061 ctgctcggcg gaataagccg gcagatgcag cttgttaaac gtctgctcgt cgaggtccgg
     3121 caaaaatctg cgcacctcct ccgccccgga aaatacccag cgaaagccgg tatgtgaata
     3181 gcgggcgttg ccgccaaact cgggctccgg cgctttttcc aacatgacga ctcgaggcgc
     3241 gccggcctgt tttgccgcca ccgccgcttc gaaagcggcg ctaccgccgc cgacaacaac
     3301 caccggtatg gtttcatgtt tcatggcaag aaattttcaa taacgtgatt taggatgtag
     3361 ggcgggttta aaacccgccc ctacgagcag attaattagc tctgtccttg ttctcgccca
     3421 ggggaacggc attggaaaac ccgtgctcgg agatgtcgaa atagttgccg tccggatcgg
     3481 aaccacgcgt ctccgcatag ggacggccat tgggccgcgg ctttggcaat ttgttgggat
     3541 taactttttt cagtctctcg acgatcttct ctccgctctc gacttcgaaa ccaaaatggt
     3601 aaaggccgtt cggcgcctgc tgctcgtgat tcggtaatat cgccaaatta aaatagccgt
     3661 cggtgagaaa gatcgcgccg ccttcgtttt tgtccttgtg cagcacctgc atttcaaaaa
     3721 cgtccatata aaaggtcgcg agcttgccgg tgtcttccgt gcgaatagca agatgtttaa
     3781 gtttcggcat ggttcaatcc tcctttgtaa tagagcacat gactaggtta ttgccgtctg
     3841 tcgatcacaa gccttgcgcc gccgcgtgcc tgccggccag ccgcgaaaag acgacgttcc
     3901 gtgtttgacc tgtgcaggat ggataattgt gaaaaaataa tccgagaata tctccggatg
     3961 caaacaggcc gcgaatcggc cggccgccgg tgttaagcac ctgcgcctgc cgatttactt
     4021 caaggccgcc gaaggtgaag gtgatgccac cggttactgg ataggcgtgg aatggcggct
     4081 catcgatgcg cagtgcccaa ttggatttgc gcggcgtaat tccctcggtg cagcggccgt
     4141 cggcgcgagt ggcatcgaac ggcatctcac cgcgcaccgc ccggttgaac tcgtcgaccg
     4201 tgtgtctcag gatcgcggca tcgatgccga tcttcgaagc gagttccgcg atcgtcgaag
     4261 cttcaacagg agtggcaaac gcataatagc tcttatcgca aaacgccaca cctttgcgat
     4321 cgtaaatttg ataggcgact ccgcccggct cacctaaaac cgcccatccc gttttcgcat
     4381 aggtgtagga gtggtgcgcc tcgccttcat cgaagaaacg ctgccccagc gagttcacgg
     4441 taatgccgaa ggtatagccg taacgattgg ctttgttgct aagctctaca tcgggaaatg
     4501 tcgcatcaat cggtgtggca tgcgccccct gccagtggcc ggacgccttg cagcctaaac
     4561 cgagcgccat ttgcaacacc tcgccggtgt catggcggct gccgcgcacc ttcatcagat
     4621 ctgcattcgc acccaaatat ctcgcgcgca tttccggatt ggcttgaaac ccgccggagc
     4681 agagaattac ggctccggcg cgcaactcgt attcctcatt gaaatccgac acccggacgc
     4741 cttcgatatg gcggtcattg cccaaaagtt cacgtacccg agaatcaaag taaatttcaa
     4801 ttcccatgcg gcttgcgatg gcacgccatt gattgagttg actgaccccg ccgccaactc
     4861 caccggtagg atgaatcaca atcccgggtt cgaagtagcg tttgccatcg atctcgaccg
     4921 agccggccgg ctcccactga attccaagtt cgcgcatcca gtggatcgct ccgttcgact
     4981 cattgaccaa cacttcggcc aaatctttgt tgatgcggcc gcgggttacg cgcagtagat
     5041 cggcggtgaa atgctcggga tgatagccgg gtaattgcag atggctaaag cttgcctcat
     5101 ccatgtcggg caaaaaccgg cggatctcat ccgcccctga ataaacgaag cgaaagccgg
     5161 tatgagagaa gcgagcattg ccgccgaatt ccgcctccgg tgccttttcc aacatgatca
     5221 ccgacggcgc gccggcctgt ttggcggcaa ccgccgcctc gaaagctgcg ctgccgccgc
     5281 cgacgacaac taccgaagca tgcttaattg tcatagaaac gtgcggagga tcacgaatgg
     5341 tcttaacaat ttcagttaat gcttcttcga gacgctttcc acgaagccgg agctatcgag
     5401 ttttctgata aacgagtcat cgaccacggt atcgatcttc acaccggcca gcttcggatt
     5461 aatggtagta agcaagccgt gcaggcgttg cattccttcc ggtgaaggat agggcacccg
     5521 ttcataagaa ttaatgactg actgataagc ttgctcgctg tcactcggat tggataggcg
     5581 caagttggaa gcgatggtgc gtgtgacgct ttctttgttg gcgggatcga acatcaccgc
     5641 gatcgcttcg acaatcgtct tggcgtaggc gtcgacgact tggggtttgg tgattgccaa
     5701 tcggcgtgtg gtgacgagca tagtttgcgg catcgtcact cccgagcctt tgaaattgaa
     5761 catcaccgga tagcccttct gcccatagag atcgccgacc gagccgttca gtgtcgtggc
     5821 gtcaatactg ccgctttcaa gtgctagccg gcggttgatt tcggtgcccg aaatcgtaaa
     5881 aaaagcgatc ttggctgcat tgggatcgag cgccaggtgc tgcaatgcca gcaatgaaac
     5941 caaatgagat gttgagccgg ggccgctgat ggcgatccgt ttaccgcgaa gctcttcctt
     6001 tttcttgatc tcgggcctcg tgttgagacg ataatcgagt ttctgcgcga aattggctat
     6061 cccgatcaca tcggtgccgc cggcggcggc gttgaccaag tgtcccggcc cgatcaccgc
     6121 catatcgatc tcgcctccga ccaacgcgga aacagccaac ggcgaacgcg gcattagaat
     6181 gtattggaca tccagtccgt acttcttgaa caggccgcgg tctctggcaa tccagaccgc
     6241 ggccatgctt tcactcgtga tgctcgaaga taaattggat ttaatctggg caggagattg
     6301 agtcggtaaa aaaccgactg ccaaggcaaa gacacaaaga aatttccgat ggtaagtagc
     6361 catcgaaatg ttgcctccct ctgcgtcgaa gggtcttaat tgggcttctt ttcgcccaca
     6421 ggttcaacag tcagaaatcc atgctccgaa atgtcgaagt aattgccgtc aatgtcagaa
     6481 gtgcgggttt cggcataggg tcgcccgttc ggacgctgct taggcaactt gtttggattg
     6541 aatttctgca atctctctac gatcttttct ccattttcga cttcgaagcc gaaatgatac
     6601 aagccattcg gcgattgttg ctcgtgattg ggcaggatcg ccaaattgaa atatccgtcc
     6661 gtaagataga tggctcctcc ctcattttta tctttgtgca acacctgcat ttcgaaaaca
     6721 tccatgtaga acgtggcaag ctttccagta tcctcagttc gaattgctaa atgacgaagc
     6781 ttaggcatgg ctttcctcct gcgcgaattt cattcaaccc ttatgcccga ccgatgagct
     6841 tgtcaagcca ggtattcccg cgctgtataa accaatttcg ggaagcgcga ttgacggctg
     6901 attgatcgag aaaaattttg cgttgtgtcg actacgaatc gacggaagtg cgccccggtg
     6961 cactcgacca gctgtgacgc atgcgacggc aagtcttacc gaatgagagg ttcaataacg
     7021 aacggaagga gtaactcgca aaatcatggg tgacggcggt acttgttttc caacaggagc
     7081 taaccatgcg cggaatgttc ggtttcgcgc tcgacgatca gcggcaacat cgcatctcgg
     7141 atcacccgat cgctgacctc gccgaagcgt tccttgaaac ggtcgagcgg aatcaccgcc
     7201 ttagccatgg cgcggtggcc gccggcgctg ccaatctcgt cataaagctt tttcatgatt
     7261 tctccggcgc ttttgacata gccgacgttg cgcacggaaa taactactcg ctcgttcacc
     7321 agaccggaga caacgcccca ttcaacacct tcgatctgca agcaaaactc ggccatttgc
     7381 gggatcacat cctcacggtt gatttccccc atgtggatca cggcaacgcc attgtcgagc
     7441 cgcaaacggc taagcgcgcg gccgagcgct tcgaggtctt cccgcggcaa agccgggcgc
     7501 tcgatccgcg agatcattgc cttgttcgca aacggataaa gataggtaaa agcgctgaga
     7561 tcggcaaggt tgctgcccct ctccagaaac aacgtatcag tcttgatgcc gtagagtagc
     7621 gcggtggcga ggcgctgcga gggttcgata ccggcagcgc gcaggtactc ggtgaaaatt
     7681 gtagaggtcg cgccgtatgg ggttcgcaga tcggtgaacc gggctttgaa gttggctcgt
     7741 ttaggatgat gatcgactac taaatccact tttcccagcg gtttgtcaaa atagggcggt
     7801 tgcacgtcga ccattgccag cttggccccc tctttatgca agtcgtcatg ggtgatccga
     7861 tcgatctgaa cgtccagcaa ccggatcata gcgaggtttt caggtcgggc gacgccgcca
     7921 aaagtcacaa tcggcgtggt tgcgcgattt cttcccaaga gttcgcgcag tgctagagcg
     7981 ctgccgatgg cgtccggatc gggatcatgc tgcaaaagaa tatgaacctt ttcttcacca
     8041 aaaagcgccc tgatgctttc aacttttttc cggttaaggg tgtctaggag gtgtgccgcc
     8101 caaaactccc ggaaggtatg ggtcagctga atctgccgaa cgagcgggtc gttctcgtct
     8161 ttcttacagt ttttgtcgtt ggtaagtaaa accacgggca aagccttgtc catggttcgc
     8221 agcagtttgc agagtcgatc ctgagattca gggtcacgaa gaaagacaac cgcttggtcg
     8281 atatgatgta atttgacctt cttgtaagtc tcttctttga ggagatctcc gtaccaggcc
     8341 ttaccgccca tacgcttgat ccgacgcgca acggtctcct cttgaactag aaagcgaacc
     8401 tcgccgtgtt cagttgcgac gtcgcgcata aactggaaca ggagatcgtc ctcgcagatg
     8461 gctagatatt gcatggtcgt ccctacgagg gactatcgag gaagcttgca gccggcgagg
     8521 ttgaacgtgt agacgctcgt taacacggcg gaaactttag cacttagccc tgagggaatc
     8581 cagtctaggc tcgacgatag gaacttagga tggcgtgata ggcgcgacaa acaacatggg
     8641 agcgcggccg gcaaaccccg aacctggttg acggccggcg cgcaacccag atcctgtgaa
     8701 accgagtact acttacctcc tcgctaaaat tagcattgtc ccgggcaatg ttcctggaat
     8761 atcgccatgg gtttcgagtg ccccaaatcc ggcctcgtcc atccattccg catactggga
     8821 tagggtccag cattctccct caggagtgtg tgtcagcgta taaaccgaaa agcgcagggc
     8881 aaaagtcggc tccgttctct cttcattggg cacgaagtca ttgacaatga agtacccgcc
     8941 cggttttagt gcgctgtaag ccttggcgaa cagctcttga ttccgctttg gcgactcgaa
     9001 ccggcagaag ttgctggcga gaaccaggtc gtaatggttg gtttcaattc ttgcggagtg
     9061 gtgctcgcca tcgatgaagc ggattttgcc ttccagaccg cgctctttgt tgagcttctt
     9121 cgctacggcg ttgacgttag gccaatccaa ctgcgtcact ttcgccgatg gatcccgtga
     9181 aagaatcgtc attccgaaga tgcttgaacc gccagcgatg tccagcacgt taacggcttt
     9241 acgctctgtg ccgatcttaa gaacatcgca tagggctctg gcggcaggct cggcaataac
     9301 aataattccc agaaccagct tttcccagac ttcaagcgcg tcgaccgtgt ccaagcggct
     9361 gaagggtttc ccggtcttga gggattcttt gagatgagcc atcccgtccc acagcgccag
     9421 tgcaacatgg cggaaatcgc cgacataaga aggtttacct cgaaccaaaa aagcttcgct
     9481 gaccggcgtt agctggtaga gactatttcg cttttggacc aacgccgtgg cgagcaggct
     9541 atcgagcacg atacgagtcg cccgcggatc ggttccggcc tgtctagcga tttcgttcgc
     9601 cgtgtggaag ccgttgtcga tcagggtaaa aaaatcgtac tcgatggacg cgtcaagaat
     9661 ctggctattg gcgaaatcgc ccaagatccg catcaaggac cgcggtgaaa gatcggcttg
     9721 cgttgctggt aattcaggca tgatcaggtt ttgtacagac cttccttttt gatgtgctgg
     9781 taaacacctt cgaaaaacca ggcgaagtaa gcggtcccca ttcgcaggca atacgccagg
     9841 ttgaatcccg gtcgagtctc ggcgcggccc tcctcgagca gctttctcaa gaccgtccaa
     9901 tcgagctcgg catgcgccat gacgccttgg tgttcctcca gatcggcttg agcatggacc
     9961 cgaaagtaaa cgactttttc ttcgggcaaa tggtaatttt ggcgcaacgc gctgccgaaa
    10021 aacctggccc aatgtccgac ggcttcttct acgcagaaag aagcccacca ttcggccatg
    10081 gtgccctcgt aaagcacacc tcgataatat tcgagaaaag cgcggcactc ggcgagcatt
    10141 tcgtattcga tcatctgatc atgggtgagg ccaaaatttc ttccctgttc aagaacgatt
    10201 tgaatgtgac ccggcggcgt cggatggatc atttcgtcgg caacgtgatt ggcaaaatgt
    10261 ttttgcaatt ccgggtgccg tttgaagaaa ggcaattggc gttggtacgc cacgccatgg
    10321 aaattattga tttcagcgac aaagccgtac cagttttgcc agaaaacctt aatcgcctcc
    10381 attggcaagg tgccggcgct catgtctgta tgaaatcggc ccttaaacag ccagcgatcg
    10441 gccaactcaa gcaaatattt ttttagatcc gcgattgccg catcggcttc ttgcggcgac
    10501 aatcgctttc ccgagtaatc tttaccctcg agctccggca tatcgttctc ccgttaaatc
    10561 aataaagcgc gcccgggttc aagatattat taggatccac cagttgtttt atctctttca
    10621 ccaggtcgta taagttagtc aatctttcat agggtaacgg acccttggta ctcagcgtgg
    10681 gagcggtcca aaccacccca cgctcgttgc cgaatcgttg caccatgctc atccatttgc
    10741 gcagcttgcc cacgtcttcg tcatcgtagg ggttatagaa aacaaaatat tcttctttga
    10801 tcgccagatc gcgcgtaaca taactatcat gctgcggcga atgttcagca tcccatatgc
    10861 cgcactccct ttttgccgcc tcatcgagat cgtacatctc tttcaagaac gtcaatgggt
    10921 acatcttgga agtcaccgca aacatgccgc tgtgacgcgc gaccgcgccg caccaaccgt
    10981 ccggtcccat gacgtgatag tcgggccagc cgtgcagccc gtgctcttgc gccttggcgc
    11041 ccatggcctt cgcggcaatc tccggaacgt caatgccgcc cgcgttatgg taaatacggc
    11101 gcatcctttg ttctttgaaa gcgatttctt ctgcgtcccg cgccatgata acggtcttca
    11161 aaaaaaacca ggcgtcatcc ggaatccggc tcttatggta gagtccttct tccattggcc
    11221 aatgatattg ccagcggtcg gtgaagtgga tgtcgtaaac gacctcactc ttgacgagct
    11281 gcatcaacgc ttcttgagca ttcagcagct ggtcacgacg aaacgtgtag cagtagtcat
    11341 gcagccactc gcgcttacct tcaatggcgc gcaagcatac tttgctaatc attcccatgg
    11401 aacccgctgc tagggtaaag aggccgagca aatccggcaa atggatccat ttctgaaatg
    11461 gaccaaatgt ggttccttga ttcgccagcg ctcccaacct gacggtttcg ccattggcca
    11521 gaatcacttc caaaccgaga accatatcgg caatccagcc gtagcgcgtc ataccgtagc
    11581 cgataccgcc cttattgatc atcgcgccga tcacgaccgc aggaccgtaa gtgccaaagt
    11641 taggcagcat caggccatgc tggcgtaatg catggtgaat cgcataaacg ctggcgccgc
    11701 cttcggcgat aatgtattgc atcgaggggt tcacttcgag aatcttgtcc atgccgagca
    11761 tgtcgagcag gatgccgccc ttcagcggtg agctgattcc cattcccacc ccgcctttgg
    11821 cgacgatcgg gattttatgc tcattggcaa agctgaggat ctttgccagc gcatgattgc
    11881 cttgggcgtc cgattcgacc cgtacgatca cgtccggcaa ggctcggtaa gcgccataag
    11941 cgagcgtgtg ctgataattg attttgtcga tcgggtttgt cgaaaccaac cgaccgccgg
    12001 cgatctcctt aagtctgacg accatctcat cgagactgag catgcgattc actgcatcat
    12061 ctaattggca gagccaggag aaaaccggcg gcatagacaa caacacacga tccgttccgg
    12121 agcgaactcg agctgctgat gttgcggttt agagctgaag gtgactgccc ccggtaatca
    12181 ggacatggct gccggtgagc atactcgctt ggtcgccggc caagaaggcg atggcacccg
    12241 ccacatcttc aggatagaga tgctttttga gcggcctgtt ttcacggacc ctttctctat
    12301 acgcatcagg cataatcgag gacgcctcgg tcaacgtaaa agccggtgtg acaacattca
    12361 cgttgatccc aaccggcccc aactctatcg ccatggtgtc agccatagat tcgagggccg
    12421 atttcgcagc agcgtaagcg cccatcttgg gcagcggctg ttgcgccagg cgcgtgctga
    12481 ccacgacaat cttaccggat tttttctcga tcatatccgg caagcaggcc tgaacgcagt
    12541 tatggaaagc gatgagctca tgttcgatgg cgcgatgaaa gtcgtcccaa gataactcgt
    12601 gcagctgacc cacctgaaaa ggaaagtaag cgttgttaac cagaacatcg atggcgcgat
    12661 aatgctcccg aacgcctttg atcatcagat caacctgatc tttgcgcctt acatcagctt
    12721 gaaagaccat tccctcgccg cccgcccctc ggatttcgtc aagaactctt ttcgcggctt
    12781 cccggtttcg aagatagttg atgatcaccg atgcgccgga ttgagccaac cgcttagccg
    12841 cggccgcgcc gatgcctcga ctcgcgccgg taacaagaac gaccttttcc cgaagctgca
    12901 aatgtttgtc ttgctcggtc atggagctaa gagcaattag atcgccacgg catcttcgga
    12961 ggcgcccttt tcgtatttgg ctgcgttatt cttgaaggac aggaacttca tctttacata
    13021 gggagaagta taaccattta ggatccgctc gatccggccc ggtttactgt agaaggattg
    13081 aaatgcccag ccggtgagtt cgccgagttg ctgacgcgag agatacttgg tcggcatgac
    13141 gcaatgctct aaatcgtaca agctcaggtt gtcgatctcg atcagatttt ctttcaccgc
    13201 tttcttgtag atcggcgatc cgggaatcgg gttgagcaac tggatcataa cgatatccgg
    13261 atcaatttca tccacagcct tggcgcgctg acgaatcttt gcctcatcat cctcccaaaa
    13321 gccattcaag taggtggaga tggtcgagat atcgttctta cgcagaaatt tgataagttc
    13381 ttttagttcc tggactccca ccttcatcgg tcctttgcga gcttcggcga tttcctcatc
    13441 ggtcccgcca tcgataccaa tgagtacctg gtacagaccg acttttctca aacgcggaat
    13501 cagatcggca tcacgaatca agttctgcgc tctgctgagc ataaaccaac tcatgtccaa
    13561 tcttttcttt tccagctctt cgagcatatc taccatcttt tgtcgatcgg aattgaaatc
    13621 atcgtccatg aaagtaagcg ctttaactcc atatttctga ttgagaagct ccatttcctc
    13681 cacgaccttc ttgccgccct tggttcgatg cgagctaaaa tcgcgaggat tacgctgatc
    13741 gaccagccac cattcataac aaaagtggca agcgccttcg caacctctcg aagttacggt
    13801 ttcgttgtaa ttcggaatca ccgaataccc acagtaacgg tccatcggaa agagatcgta
    13861 agccggtaga ggcaaatcgt cgagattaga aataagcggg cgcggcggat tgagaacgac
    13921 ttctccattt tcacggtaag ccagcccttt gatcgttgca aatctcggag acggcatttt
    13981 caaggcttcc aacaattcgc aaagcgtgtg ctcgttatcg ccgaacacgc cgacgatcgc
    14041 aaaatccaat tgaggattat cggccatgat cttctgcgga acagccgtgt acatcaaacc
    14101 accgccgacg gtaatagtgt cgggtagaat atccttgatt cggctcatgg ctgcgtgaaa
    14161 ccgattgagt tgcgcggcgc cgcccgcggc agcgatcatg ttgccaaaaa aagccacatc
    14221 ggggcgggtt tgtttgattt tttcgagcat gccatcgtca tcgagtgcta gcgcgcggca
    14281 gtcgagcacc tcaacctcag tagcgttgtg ttctctgacg aagcccgcaa gctgcgcatg
    14341 cagtgagttg acagcgacgt ggcgatcacc ccagggggcc cagttgccca tggggggcgt
    14401 aacgaaaaga attttcatgt atctcctcga cccgcggaag gaaaattgat ggttgaaacc
    14461 aaaatattta agacctcaac gattgtcaag attcgcgtat aaatcagctt gtcacgacct
    14521 ggaaccgtcg cgctgaaaag cggctttgat cgggccagca ctagtattga caagcactac
    14581 cccgtttgct acacgttcgc gttgctgccc gagagtgact ataaaccacc aggaggacaa
    14641 aatatggctg atcaagccgc agaggagcaa cttttagacg ttagcgttgc ggaggcatct
    14701 gccgcctata agttctatct gaacaatgaa aagaagatac taggcacctc ggcggtcgtc
    14761 ttatttttaa ccgcctggga gttggttgga aacgtgctcg ggatgattaa cccgatgttt
    14821 atgagcgcgc cgtcgctgat cttcaaagcg gcgattcaga tgttctcatc gggagagatt
    14881 tataacgatc tatatgtcag cgggcttgag ttcgcctggg gctatttgtt gtccgtcgcc
    14941 gtcggagtgc cgtttggcat cgcctgcggt tggtataaga agtttgctta catattcgat
    15001 ccttttatca atgccatgaa cgcgacgccc agagtcgcgt tgttgccgtt ggttatcatc
    15061 tggctcggga tcggcatcct gtccaaggtc ggaatcatct ttcttggcgc cgtctttccc
    15121 atcttgatca atgcgcgcga cggcgtaaaa accaccccat acaacctctt gaccgccgca
    15181 aggagcttcg gtgcttcaga atggcagatt ttcaaaagcg tcgttctgcc ctccactctc
    15241 ccttttattc tcaccggatt aagactcggg gtgggcaggg cgttgatcgg cgtcatggtt
    15301 ggcgagcttt atgccgcaac ggcgggcatc ggctttatga tcacggtggc gggtgccacg
    15361 tttcaaaccg ataaagtatt tgtgggcgta ctcatcttcg cgatctcggg aatgatcgcc
    15421 atggagcttc tgacccggct cgaatcccga tttgacaagt ggcgtcccaa ggtcggagct
    15481 gtcgcttaag cagccatcac gcaccatcag ggtggctccc gaatcgggag ccacctgttc
    15541 catccggcat ttccttccac gaacaatcaa tcgctcgtag cttagcatcg ctcttgatgc
    15601 tattccctac ggaactcgtt ctaatcgggc atcctggaaa attctagctg aacagaggca
    15661 atctcatgaa gggactgggt gggctattcc tattcgcaat ctttttagtg ctgatttcca
    15721 ttggtcaact catttcgctt tatgtcgatt ggctctggtt tcacgaagtg ggttatacgc
    15781 aggtttttac cactagccta tttttcaaaa ctgttctggc tctcgtgggc ggtggtattt
    15841 ttgcgctcct gatctacgct aatgtccgaa tagcggctcg gttggtcaga ggaatccggt
    15901 tcctcgatca agagaacttt cttgacctgc cgcgacctga gctaatcgat cccttagtcc
    15961 aacgattgct tctacccgcg gcattggttc tggggctttt tgccgcgccc caggctgcca
    16021 agcattggga atcattcctg ctgctgctca atccaacgtc ctttggccta gaggatccgt
    16081 tattcaataa agaccttagc ttttttgtgt ttcagttacc ggcgctgcgg acactctaca
    16141 attggctgat ggcggctttg gcgctttctc tggcattgac cgcgatcgtt tacgtacttt
    16201 atcgaagcct gcagtatagt ccgcgcggca tttttctcgc cccgcgggcc agactccact
    16261 tgttgatcct gatcgcagcg ttatttgctg ttaaagcagc aggctatgtc ttggatacgt
    16321 acgatctgct gtatgcttcc ggcggtgccg cctttggcgc tggctatacg gatgtttatg
    16381 taagactccc ggccttgagg atcttgacgg tcgctgcttt gatcgcggct gcgctctccg
    16441 tcgctcagat cactcgcccc ggttttggct atctgcttgt tggcatagcg gggttggttg
    16501 tggtccatgt gatcggactc agcgccgccc ctgcgatggt tcagcacttt cgtgtagttc
    16561 caaacgaggt ggattcggaa cgtcccttta tcgaacacaa cattaaattt acccgaaccg
    16621 cctatggcct cgataaaatc gagaacgagg attttccggc tgacgaaacc ctgaatgccg
    16681 ccgaccttca acggaatgat gcgacgatca aaaacatacg cctttgggaa catcagccgt
    16741 tattggcaac ctacgcgcaa ttgcaggaaa tacggaccta ctacaagttt gtcgacgtcg
    16801 acaacgatcg ctataacatc gacggcacct atcgtcagat catgctttct gcccgtgaac
    16861 tatcgcatca acacttgcca agtcggattt ggatcaacga acatttaact tacacgcacg
    16921 gctatggggt tgtcttcggg ccggtgaatc aggtcacgcc ggaagggctt ccggagtttt
    16981 tcatcaagga tataccaccg gtttcggagg gcagcatcaa ggtaacacgg ccggagattt
    17041 actacggcga gctcgccaac gattatgtat tcgtcaagag tggcgcgcga gagctggatt
    17101 atccatcggg cgaccagaat atctacactc agtacagcgg cagcggcggt gtatccatcg
    17161 gctcgttgtg gcgcaagctt atcttttcgg cgcattttgc gactttaagg atcgtgctct
    17221 cagaggacat tgttcaagag agccggattc tatatcaacg gcaaattcaa gagcgagtta
    17281 aaaagatcct gccgttcgtc accttcgatc gcgacgctta catggtcatt gcccaaggcg
    17341 gcaggctgtt ctggatgatc gacggctaca ccacctcgga tcgatttccg tactcggcgc
    17401 cgatacgccg tcgaggcacg aactatattc gcaattccgt caaggcagtg gttgacgcct
    17461 acaacggtac cgttgacttt tacatgagtg atcccgacga tccgatcgtt caatcctatc
    17521 agaagatttt cccgcaactt ttcaagccgc tcgaaaaaat gcctgaggat ctgcgcgcgc
    17581 acatacgtta tcccgaggat cttttcacca ttcaagctca aatgtacgca acctatcaca
    17641 tgcgggatcc gcaggttttt tataacaaag aggatttgtt aagcatcccg cggcagacca
    17701 caagcggcag cgagcgggaa atggcgccgt actacaccat catgcgtcta ccaggcgaga
    17761 ccaaagagga gttcgttctg cttctgccct tcaccccgaa caatcgtgac aatatgcgcg
    17821 catggctggc cgcgcgcagc gatccaccgc actatgggaa actcatcgca ctcgattttc
    17881 ccaaggcaaa actcgtttat ggtccgaaac agatcgacgc ccgtatcgaa caagacgcct
    17941 ttatatcgca gcaattgagt ttgtggggac agaggggaca ggtgatccgt ggaagcttac
    18001 tggccatccc tatagaaaag tccctcctct atgtccaacc cctattccta gcagcagaaa
    18061 aggggtcttt accggagcta aagcgagtca ttgtcgcctt cggcaaccag atcgcaatgg
    18121 aagagactct cgatcaatcc ctccaacgcg tgtttggtgg aaaggcaagt accgagccta
    18181 cgccagtggc ggcgagcgtt aaagaagccc ggatcgacag tactttggcc caacgggcgc
    18241 tgcagcattt ttcccgttcc caagaatttt tacgtcaagg aaattgggcc gggtatggtg
    18301 aggagcagaa aaggcttgaa gcaatcttac gtgatatgca gcaaggacgt taatcaggca
    18361 atgagtcttt tgcatcctga aacggaatcg gcaagagtgg aaccgtatcc gcgcacatca
    18421 tatcgcagtc agtgcttatc agaaatcaat tgagccagat tgctggttga atcgctcacc
    18481 aagcgctgat gaagcaaatg cacccgtcct gggattgcag caactccctc gacctcgttt
    18541 cttatgaatt ttactgttat aaagccgcga tgacggggca acagcgacag ctcgcaatca
    18601 ggggtttgat ttttgttgtt ctgatcggac tgttgtttct cttcagccgc tatttaccgc
    18661 aaccgttttc cgctttggtt ccgccgggcg aggagattga tgacttcgaa gggatctatt
    18721 ccagcagcga caagttaggt aactttcttc gttcgctcgg tccttattcg ccggcggtat
    18781 tcgttctctt ccaggtttcg caagtcattg ccgcgccctt tccaggcgag ctgaccggag
    18841 tggcaggtgg atacgtttat ggggaaactc tgggtttcat tctctcgacc ataggactta
    18901 cgctcggttc atgggttgcg tttgaattgg caagcatttt agggagaccg tttgtcgaac
    18961 gttttgtcaa acaagatgtc ttgcaccggt tcgatttttt aaccaccaat accggagcgg
    19021 cgatctgctt tctattattt ttgtttccgg gttttccgaa ggattatctc tgctatgtgc
    19081 ttggcctgag ccgaatgaag ctggcgacgt tcttgtttgt gacaatcgtt ggaagaatcc
    19141 ccgggactta ctttctcacg atgcaaggcg ccagcatccg aaatcaggag tactacacgg
    19201 caattatctt cgccgtggtc tccgcggtta tcttgctaat cgcctatctt tatcgcggtc
    19261 gaattttcag ctggatgaag caccgccacg aaatctaaag gcgtcaaatc ggccgctggc
    19321 acgcacactc ttctgcgccg gttcccagtg catcagctag agcctcgttt caccggtgag
    19381 aagaaaaaaa attggagcaa caataatccg gtaccaggcg aacggtacaa gagtccattt
    19441 actaaggaaa aaaataaatg ttttcacagc catcagcgcg acgataaacg aaacgaggaa
    19501 tccaatcata aaattcgtcg cgtcttgggc gctgaacaac gtgcccattt tcatcaattc
    19561 ataacattca acgacaagca taatcggcac cgcgatcaaa aacgaaaatt ccgcggctcc
    19621 cttccggtca aggcccaaaa gcatgccgcc gacgattgtc gcagcggatc gtgaagtgcc
    19681 gggccacagg gcaagaacct gaaatagccc cactccaatg gcctgggcta ttgtcacggt
    19741 ttccagcggt ttcgacgttc ttcgcttaag cagacgttcg gcgattaaca tcgctagccc
    19801 acctccggcc aacgctacag ttaccgccca cggtgtgaaa agatgggttt tgatcaagtc
    19861 tttcgttaag aagccaacca caagggcggg taacgttgcc gcggttaggc gccagatccc
    19921 cgcccatccg aggaggtttg aatcttcgca tcgaaatcga gtggagttgt tgctcaccgg
    19981 gagcaaatcg gtaaagcgac gccaatacag cagcagcaca gctagaatag cgccaaattg
    20041 gatggcaact tggaagatcg cggccttttc tccgaagaag cctagaagat ggctcgcaat
    20101 gataagatgc ccggtcgatg ataccgggat gaattcggtt aacccttcaa ccacacccaa
    20161 aattacagca actgcccagc cttccatgtt tccctttcta gtttactgcc ctaggacgaa
    20221 aattcgacct gcggagtttc cctattcatg cccttgattg tcgctcaatt ggcgattcga
    20281 catcagcacg tactcatcaa tgctggttag cttgcgcggc gaagctccga tttccctctc
    20341 catgcgagag atattttttt gtttgacgat gatcagcaac ggcgtatcgt ttctttccca
    20401 gactcgacga aattcatcca agtcaaaaag tgcctttccc cagcgcgtgt cgggccaagc
    20461 ccgatgagta gccacataga agttaccgag cactgtcttt ttcttgttag catgggtgac
    20521 gatccaaaca ggtctttgcg cacgtagata gaagggcatt cctgtaagat aagtgtcata
    20581 caaaaccact tgggtgtccg cataggttcg cgcggcaacg gcgccggcga tttcttgggc
    20641 tgacctgtcc cgtgatacgg agaccatgat ctgcgcgagg aggaccgaga agaaaaacgc
    20701 tcctgcggca tgtagggcaa agacggttga ctggcttcgc caaagttgag caatcttacc
    20761 ccagccaagc gcgatcaaaa ccgcaaggca gataatcgca tagacgacga tggaccattt
    20821 catagaaata aaagcgccgc gaatccggtc tggcagaatc accggccaga tcaatcccag
    20881 gacgaggtac caaatgctaa ggctctgtag cgcccaaaaa aacgcaagcg gccgcttgag
    20941 gttatgagcg cggttctgaa acatgctact tagcgtggtt gcggtcagga tcgacaaagc
    21001 gggaaagatc ggcagaatat aatgcggcag tttcgaacga gagaccgaaa aaagaactaa
    21061 cggtacggtg ctccagacta tcagaaagat ttttctgtca tccaacgatt gtgaccagta
    21121 ccgtcgaatg acgctcggaa gcagcgcgct ccagggaaga aacccaacaa cgaggactcc
    21181 taaaaaaaaa taccagggtt cttcccgatc aaagctagct gtcgcaaagc gtccgaagtg
    21241 ttcgtcccat agatagtaac gaataaaacc ggagttacgg gcctcggcca gaagatacca
    21301 cggagtaacg agcgccagaa acaataatgc accaggaatt agataaaccg agccaagatt
    21361 tgaccaccgg ttcgcgatca aaaggtaaaa gaaaatgatc aacccgggga tcagcaatcc
    21421 cactagacct ttgattagcg taccggcggc taacgcgcca tacatcagga ggcagtagat
    21481 cctgcgctgc atcgccgcgt cggcatgcgc tgcttggtaa aacgaataga gtgccaggct
    21541 gatgcaaaaa gttaggctca tgtcgaagat gacgacgcga gacaaaataa aaaattccac
    21601 gccgctgatc agtatcagcc cgctccatag cgcttcccag ggaccccatc gaattcgtgc
    21661 aaaactataa accagcgcaa agcagcccaa tgccgccagg acagatggta atcgggctgc
    21721 ccattcattc acgccgaagg ctttgtaaga gatcgcgatc agccaataaa aaaacattgg
    21781 tttgtcaaga accggaaaga aatcctcatg cggcgttacc caatcgttta gtagtagaat
    21841 ttctcttgct ttctccgcgt tccgaccttc atccggttcg aacaagcccg ccgttccaag
    21901 ttggtagaag agagatgtta agccgacggc taacaacagc cagggcaacc agcggcgtgg
    21961 cggctcaatg atatcggact cgagtcggat gttgattgaa ggttgcgtag tagcgctcac
    22021 cggaactgat ctgtaggacg tgttcgacaa ggtttacccg agaatcaccg aattacaaga
    22081 taacagcgag ttgcgcgcaa ccgtccgtag aagcctggtt atgggatcat ggattgagat
    22141 tgacaacctc ggggggctga cgtagcttga gccgtaatca gggggtgcct tatgtttgga
    22201 cttatccgaa tcccggagct gcttatcgtt tcggtgatcg ccctcgtggt cttcgatccc
    22261 gccaactttc ctgatttccg aaaatctctg ggcttatcga ttcgtggttc aatgaaaaca
    22321 ctaaccgact ttgtcagatg ggcgattgaa agatctggca gttcaacgcc agtcaaccag
    22381 gccttcacct tccagtttgt gccgtaaagt ccggggttct cgaccaatga agcggcaccc
    22441 aatcaaagtc gcgacaatta tacttttatt tctgtcgaca tttctaacgc caacttccaa
    22501 tctcctttat gcccaacaac ccgtagccag ctttttctac gatgatcaag gacatgtgac
    22561 gcaacaagca cgggacacta atggggacgg caagatggac cgttggaccc attataatga
    22621 gcgaggacaa atagaacgag tagaacaaga catgaatttc gacggcaagc cggatgcgtt
    22681 cgtctattac gagaacggca agccccggcg ccaagaagtc gctaataata acgacggacg
    22741 aatcaatctc tggtatctgc ttgatccaga tggaaaagtc gagcgcaaag gcgaagatcc
    22801 aaatagaacg ggtaagccaa ccgtgtgggt ctactatcaa gacggccagc cgctacgggc
    22861 cgaagaacag aacggtcctt ccggcgtagc caatcggcat gtttattttc gcgatggtaa
    22921 aataagtcgg gtcgaggagg atacggacgc cgatggtaaa cccgatagct tcgcctttta
    22981 cgacaacggc cagttggtca gaaaagagct ggaccgcaag cacaccggca aaacagatct
    23041 ttgggggtac tatcaagaca accgattggt gaagcaggaa gaggaccttc gcggcgacgg
    23101 cacagtcagc cggattgttt atttccaaaa tcagatcgtt gtgcgccggg aggaagatac
    23161 gatggggcgg caacgaatgg ataccataga ctcgtttact caaggcaagt tgaccaaacg
    23221 tctacgggac accaaaggca caggaaaatg ggacgcggtt tatgttttcg agaatgacca
    23281 ggtcgtacgc gaagaacggg acactgacgc ggatggtatt ttcgacttgc gaatcttcta
    23341 tgccgatgga cagatcaggc ggcaggaagg ggacaccaac ggcgaccggc gtgtcgatgt
    23401 ttgggttata ttcgagaacg gggaacgaat cgagcagctt gaggaccagc attctcgcgg
    23461 gatgatcagc gcgcgctata tctttaagaa tggacaggtc gtcgaacagc aggcaatcgc
    23521 ggatgcgcaa cccgctccga tggcgcttcc cttcgtggcg gccgaagatg agctgcgatc
    23581 gatgaccgct tccaccggcg tacagacgga cgagcgtcga gcgactgcgg caactgccgg
    23641 gctgcagtag ggtagcggaa cgaaatggaa actggcggtt agaagatgaa ccggcgattt
    23701 ggatcgatat cgtacgtgat cttattaaca ttgctcgggt gtgaacctgc tgcagttcaa
    23761 tatccgccgg gctctgttca accgccttat tccggacccg cgataagcaa tccaagcatg
    23821 ccgcagtacc ctacgcaatc acaggtgacc agctcgcaaa gatggaatca cgcatggaat
    23881 aaggcaatgg agggcgcctt cgtcggcctg aatatcggcg gtcctttcgg cggcgcgggc
    23941 ggattcactt tggggctgct taccggacta atcacagccg agtctcatta tggcgtgttg
    24001 aatgcgcaaa ttcagtcgga acaggaaaaa gaccggcagc ttgaagcagc gatcgaacaa
    24061 gaacttcaac ggcagtcagg ttttgagaac caggtgactc aagcaactct tacctccgca
    24121 ggcactcctc aggataaacc agccgccctg cccggggagc aagcaagcgt ggcgccgaca
    24181 gttccacccg ttcgtacggc aagtggtaat gttgcggtgg cgtcggtcaa caagagtcct
    24241 gggtctatcc ctcccacgca gttcaaaaat gtcgaagtga aagatatcaa tggggacgga
    24301 gtgccggatc tgtggacgta tcataacccg cgaaaccccg gcgagatcgt ccgtcaggaa
    24361 gagtccacga aatacgacgg cacggtcgat gcctggagct atttcaaaga tggcaaactg
    24421 gtccggcgcg acgtcgatac ccggggtcag ggaagaccgg atacgatctt ttactatgcc
    24481 gacgataaga tcgcccggga agaacgtgat gaatcaggca ccggtcggat cagttatcgc
    24541 gccatctatc aaaacggtcg ccttgctcag gtcgaaaagg atacgcgcgg agaaggtaaa
    24601 atcgaccttt ggattcacta cgacactaac ggcaccgaag aaatcatcat caaagatgaa
    24661 aaagatatga atggagatgg tattgcggat ctgtggacct actatgagaa cgggcgggtg
    24721 gtccgacgtg atgtcagcgc gctgggcttg gaactccttg ccaaacagga acagatttca
    24781 ttgccgagcg atgacgtaaa acagattcag gttcccggca actgacatta tgaacgacct
    24841 agcctcggcc tgggaagatt cagttctctc actattccgc agcgcctttt tagtgccctc
    24901 gctgctcctg cacgcattgc tcctaggtct gctgttccgt gccgccggtt tatcggtacc
    24961 acggcccgga ttcgatccgc ctatctcagt ccaactgatg gaggtaaacg agggcggttc
    25021 ccgcgacaaa agcattggtt cgaacaaagg ccccggtggt cagcgcagct tgccaaagcg
    25081 cggcactacc gtggctccag cccagcggag cggcaaattg gacagcggtt ctgtcgaaga
    25141 atcaaagccg agtgacaccg ttgaagcgac acccgcgccg aaaccgatgc cgcttccagg
    25201 ccctaaactt ctgtctagcg actcgactcg ggaggctgtc aatgtaaaag agacttcacc
    25261 ggattccctg gtgcggctgc cgaccaagga gtcggcaacc aatctgcctg gcgctgtggc
    25321 gattgattcg gaaaccagcc agaagagctt agctgcgctg aagagcacag attccggggc
    25381 agggatcaag gcgctaaggg aagccgaaca aattccgggt tctttaaaag gcgtgggaac
    25441 tggagccggt ccgtacggcg ttccgggtgg aagtaggagc ggcactggtt tgtcgggcgg
    25501 cggcactggc tccggtacag gcggcggcag tagtacaggc ctaaagggaa tcgcaaattc
    25561 tgattacaat caatatctgc agcaacttaa gagaagagtg gaatcggttt ggaagtatcc
    25621 cgacgacgtc accggaatcc aaaaggtggc cgtccgtttc attcttgatc gcgcgggcaa
    25681 gctgactttg gcggaagtcc tggattcttc tgattctaga cttaacacca gcgccgtcga
    25741 agcgatcagg cgagcgtcgc cctttccacc tattccggaa agtttgaaag atctcgccaa
    25801 tgaacctatg atcatcaggt tcgaggtcgc tatccgcgtc cgcggatgaa gactgcattt
    25861 tcatcttcct aactctcgtt gaattaatta tgaactaagg taatctcacc ggagatttcc
    25921 gaaggaatgc ataatggaac aacaagggct gaatctcatc gagatcattc acaaaggcgc
    25981 cctagcaact tatccactga tcctactttc cgtcataagc gtaacggtgg tgtttgaacg
    26041 gttatggtca ttgagaaata ttagatcgac gactctgagg atcgctgaat ccctgatcga
    26101 tccagttcaa aaaggtcaac gcgatctggc actggctatt tgcaaacaga attcgcactg
    26161 tccggcggga agaattttcc acagtgtcat cgagggagcc gaaagccacc ggcccgaaac
    26221 cggtagcaat gtcgcggtgg aagcgatgtt tgaagaaacc cagcatttga gaaaacatct
    26281 ttggatcttg ggaaccgtgg caagcagcgc gccgttcata ggcctatttg gaaccgtcgt
    26341 cggaatcatc aaagctttcg aaagtatggc ggtcagtgga accggcggct tcgccgtagt
    26401 cgcggcaggt atttccgaag cgctggtcgc caccgctttg ggcttagcgg tagcgattat
    26461 cgcgttggtt ttttacaatt acttccagac ccggattggc agcttgacag ctctgtttcg
    26521 catccaagtc gcaagagttc tccgccactt caacccctga ggcatacatg gcactgacgc
    26581 cgcttggcag caacgaagag aacccggaca ttgtcgccga aatcaacatt acccctctga
    26641 ccgacatctt tctcgtctta ctgatcattt tcatgatcac tagctcggca atgatcgaat
    26701 ccggcggcaa agtgaatctg cccaaagctg tcgctactca gtccgagagc cgcggcacga
    26761 ctgtcaccct tactccaaaa cgtgagattt tcgtcaatca gaaacagatc agtgaggata
    26821 aattagaacc ggctctcaag gaagcgctag ctggaagcgt tgataaggtt gtcattttgc
    26881 gaggtgataa ggacgtgctc ttgggcgata ccgttaagat tatgtcgatc atcaaacgcg
    26941 ccggcgcatc cgagattgcc attgccgccg aaaccgaaaa atcccgctag taggccgtcg
    27001 attctatcgt acttgcgtcc cctggtcggg tggcagcggc cgttgcgagc gtccgaggcc
    27061 atcttgcctt gcgcgttcta tatcttgttg gatctcggct tggctctttc ctaatcgttc
    27121 aacctctaac ttgaggtttt tttctctttc gaggaagact tccaactcca aagacttttg
    27181 atttccagac tgataattaa cggtaaccga gtccttgccg ttggaaccat cgctcgctcg
    27241 ggcgaaaacg tcgatctggt tacgcccttc gatcaccggc accgcggcgg aaaaaaaacc
    27301 gtcggccgcg actctcagtt ggttggcttt ttggccgctg ctctgattga cgatttgaac
    27361 gtagtccacg ccgaccgcgg atatgttctc cacgacggac aggatatcta ccggccgtga
    27421 aaccggtgta tagatgccgc cactttgttt ggcgatgccg acggcggctc ttggataaga
    27481 gagcgcctcg tcgcctaacg caaaaacgtg aaccttgatt ccggctcttc ccgccagtcg
    27541 cgccgcattg atggctaggt cagtatcttg cggcgtggcg cttttgcctc cgccgatggg
    27601 catcgtcgga aacccatctg tcaaaagaaa ctccactttt accgcatcag ttttcttttc
    27661 actgcttccc attcccatca gttcggtgat gccggtacga attccttcga ccatgttggt
    27721 gccaccataa ggccctgcct tatacacttc atcgagagcg acgcgaaccc ggtcgaactc
    27781 atgcgtgagt ggctgaacca gttttgcact ctcgccgaaa ctcaccacac ccacccgggt
    27841 cgtttgcgag ttcagttgcg gcaaaagcct acgggctgca atcacctccg ccgcaagtat
    27901 agagttcctg aggtttctca tcgcgggccc gcctacgctc atgccgccga tgaatatctg
    27961 cggagtggtg aatcctgaac cagttcccgg cagctgttcc atacctgtca aatcgacccc
    28021 ggcgtagtta gcggtactcc ctgatgcatc cagaagaagc ataatgtcaa cgttggctga
    28081 acgggccgcg gtcgtagcca ccttgccgct tacgaagact agattctgtt cctggttgat
    28141 cgtagcgccg tcttttggtg agtgaatctg cactttggct tctgcgctga ctaatggaaa
    28201 aggccggcta agcagggaaa gcacaaaagt cgcgaggaat gcacaccgga gaaaaatcaa
    28261 cttcatggcg ggtactccag tgagtagacg ctaacacgaa ccgagaacac tgtaaacgcc
    28321 acgatgggga gtcgaccgct gggaatttga tgaagatgag gcagttaaat cgggtagaca
    28381 gctcgaaaga ggaaaccaaa gcagcaacac tgtcctgacc cagagcggtc tataagcatt
    28441 ccgctgccgc tctcaagccc aatagataac tctgcacacc gaaaccggta atctgtccga
    28501 cagcgac
//
LOCUS       DASUVS010000180         4447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1383666, whole genome shotgun sequence.
ACCESSION   DASUVS010000180 DASUVS010000000
VERSION     DASUVS010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383666"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VF182_10040"
     CDS             complement(<1..806)
                     /locus_tag="VF182_10040"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6437460.1"
                     /translation="MRAIDVHVHPSTRGLDTHACNYFRRNLSEVPQSADGFADVYLQH
                     DVKALLIGWHPSTVSEGAHNSNEHAVELVSNYPKSFCGVLASLDTNSTDLPGIARYAE
                     ELVRSPKVIGFKFHPPDQGFYPSDKRFYDIWEVLQAGGKPAMFHIGFTVLGANTPGGS
                     GIGLDYGRPIHLDTLAKDFPRLKILAAHPGWPWVEETVGVVTHKQNVYVDTSGYLAEQ
                     LPELFQRAIGGRLQDKALFGTDFPYVDLGKALVSFDKMNFKDAVKEKLLLA"
     gene            complement(793..1998)
                     /locus_tag="VF182_10045"
     CDS             complement(793..1998)
                     /locus_tag="VF182_10045"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX6437461.1"
                     /translation="MLDIIRTVGVLGLGVMGFDIAFLYAQKGYRTLVYDDSQVAMQNL
                     VSRRDQTIERLKRRNRISETETESVKNGLVPVASLSDMTSAVLVTEAVSESAKIKKSV
                     YKALRKTGFTGVLTTNTSSLTRTSLLDHNEYPDEKFATTHFFNPVLYTQMVEVVRGGI
                     NDLNYATLMSFLIDLGRKPIETRDISGFVSNSVLMIYAVMALRLVEAGATIEAVDGAA
                     KEMQSLPPLFSFDSWKPSIVEDVTRVMSEFRGDDFLRSSTLLTTLAKTNPVFYSNQIP
                     NAAIYNLIEKRRSGVDPSMLKLALLTSIRVAAARTVELGESPATVDFISTEGLKLPRA
                     PLAEIDELGPEAVLDDLAKVNQAIPGGKMKPPELLTTMAQERQTFFKDGKSNPAIATL
                     LRGSAHARH"
     gene            complement(2008..2877)
                     /locus_tag="VF182_10050"
     CDS             complement(2008..2877)
                     /locus_tag="VF182_10050"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437462.1"
                     /translation="MDQIKIGIGVSSIGRFLPFIGLAAGLFEKQNILVELVNRQDEEK
                     IVDDIVAGVTPIGTPNAPSLVFSLLESNDLVIVGGVLNCPAFFLAGNHSIKSIADLKG
                     KRIGINQPRRMAGMVMLALLRRWGFDIQRDLTLVDLGLNDRSLEALRDNQLDAALLPP
                     EKAFLAASEGCHIIADSFELDFHWVPLATTRRFLSDNRALVARIAGVYVESIRIFKTQ
                     PEKTKQAIGRWLPFLANKPEVLAECYRLFADRFEESLRPSLTSISSILKNAALQDSRA
                     YGIRAESIIENVL"
     gene            complement(2882..3268)
                     /locus_tag="VF182_10055"
     CDS             complement(2882..3268)
                     /locus_tag="VF182_10055"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6437463.1"
                     /translation="MVEMKNPINEADAHPEIRHLSLWRTKLAYAWVINCEPGIQDDMH
                     YHENDDHIFMVLEGECTVRTPHKEFVLKQHDTVMLESKQPYQLCNTGKGRLLLLGAGN
                     AGVNGVPRSRVPDVASHTPVQEPVVA"
     gene            complement(3333..3605)
                     /locus_tag="VF182_10060"
     CDS             complement(3333..3605)
                     /locus_tag="VF182_10060"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX6437464.1"
                     /translation="MGLFYEDLIEPVIVKQVSKEVAYCRCTRSKNLPFCDGSHAGTNI
                     SPRMLEFDEPRTIAICRCWRSKDHPYCDGTHGRLVKPKERPKRALE"
     gene            3947..4090
                     /locus_tag="VF182_10065"
     CDS             3947..4090
                     /locus_tag="VF182_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437465.1"
                     /translation="MARQPVDHRAIVFGGDVEAMAWEMLPSQTKEQVRPSITELFDGR
                     EAA"
     gene            4245..4317
                     /locus_tag="VF182_10070"
     tRNA            4245..4317
                     /locus_tag="VF182_10070"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4278..4280,aa:Phe,seq:gaa)
BASE COUNT         1025 a         1197 c         1126 g         1099 t
ORIGIN      
        1 gctaaaagca acttttcttt gaccgcatct ttgaagttca ttttgtcgaa acttacaagc
       61 gcctttccta agtcgacgta agggaagtcc gtgccgaaaa gcgccttgtc ttgcagcctg
      121 ccgccgatcg ccctttgaaa caattccggc agctgctctg caagataacc ggaagtgtcg
      181 acatagacgt tttgtttgtg tgtcaccacg ccgactgttt cttccaccca tggccaaccc
      241 ggatgtgcgg cgagaatttt caaccgcgga aaatccttgg ccagtgtgtc tagatgaatc
      301 ggacgtccat aatcgagacc gatgccgctt ccaccaggcg tgtttgcgcc gagcacagtg
      361 aaaccgatgt ggaacatcgc cggtttgccg cccgcctgca aaacttccca gatgtcgtaa
      421 aagcgcttat cgctgggata gaagccctga tcgggcggat gaaatttaaa gccgattacc
      481 tttggacttc ggaccagctc ttcggcgtag cgggcgatgc ctggcaaatc cgtcgaattc
      541 gtatccagcg atgctaggac gccgcaaaat gatttgggat agttggacac gagctcaacc
      601 gcatgctcgt tggaattgtg cgcgccctca ctcaccgtcg acggatgcca gccgatcagc
      661 aatgctttga cgtcatgttg cagatagaca tcggcgaacc cgtccgccga ttgcggcact
      721 tcgctcaggt tccgtcgaaa atagttgcag gcatgagtat cgagcccgcg cgtgctcggg
      781 tgaacgtgaa catcaatggc gcgcatgagc actccctctg agcaaagtag cgatcgccgg
      841 attggattta ccgtctttga agaaggtctg ccgctcctgc gccatagttg tcaaaagctc
      901 gggcggtttc atcttgcctc ctgggatcgc ttggttcact tttgccagat cgtccaagac
      961 cgcctcagga ccgagctcat cgatttcagc cagcggtgcg cgtggaagct ttaaaccttc
     1021 ggtggaaatg aagtcgaccg ttgcgggcga ttcaccaagc tcaaccgttc tcgcggccgc
     1081 aacccggatc gaagtcagaa gagccagctt gagcatcgag ggatcgactc cgctgcggcg
     1141 tttttcgatt agattgtaga ttgccgcgtt aggtatttga ttggagtaaa acaccggatt
     1201 cgttttggct aatgtggtaa gcagcgtgga cgaccgcaag aaatcgtcgc cgcgaaattc
     1261 ggacatgacc cgcgtgacat cttcgacaat cgatggtttc cagctatcga aactgaacag
     1321 aggcggcaat gactgcattt ctttagctgc cccgtccacg gcctcgatgg tcgcaccggc
     1381 ctcgaccaag cgaagcgcca tgacggcgta gatcatcaat acgctgttag atacgaagcc
     1441 cgaaatgtct ctagtctcaa tcggcttgcg gccgagatcg atcaggaatg acatcagagt
     1501 cgcatagttc aaatcgttta tgcctcctct aacgacttcg accatctgtg tgtagagcac
     1561 gggattaaaa aagtgcgtcg tggcaaattt ctcgtcggga tattcgttat gatcgagcag
     1621 gcttgtccgc gtcaatgacg atgtattcgt ggtgagaact ccggtgaagc cggttttgcg
     1681 taacgccttg tatacggact ttttgatctt ggccgactcc gatacggctt ctgtcaccaa
     1741 gacggcgctc gtcatgtccg acaggctcgc aaccgggact aggccgttct taacactttc
     1801 agtttcagtt tcggaaatcc tgttgcgtct ctttagtcgc tcgatggttt gatcgcgacg
     1861 ggacactaga ttttgcatgg ccacttgaga gtcgtcgtaa accaaggttc gataaccttt
     1921 ctgcgcgtac agaaaagcga tatcgaaccc catcacaccc aacccgagaa caccgacggt
     1981 tctgatgatg tctaacatag gactttttta gagaacgttt tcgattattg attcagctct
     2041 gatcccgtaa gccctcgaat cctgtaatgc cgcattcttg aggatcgagc taatggaggt
     2101 caacgaaggc ctcaacgatt cttcgaagcg gtcggcgaag agacgatagc actcggctaa
     2161 cacttccggc ttgtttgcta gaaacggcag ccaacgaccg atcgcctgct ttgtcttctc
     2221 tggctgagtt ttaaagattc gaatgctctc aacgtaaaca ccggcaattc tagctaccag
     2281 tgctctgtta tcggacagaa atcgccgggt ggtggcaagc ggcacccaat gaaagtcgag
     2341 ctcgaaacta tcggcgataa tgtgacagcc ttccgatgcc gctaaaaacg ctttctcggg
     2401 cggcagtagg gcggcgtcga gctggttatc tctgagcgct tccagacttc gatcattgag
     2461 accgaggtcg accagcgtta aatccctttg aatatcaaag ccccagcgcc tgagcaatgc
     2521 gagcatgacc atacccgcca tgcggcgcgg ctggttgata ccgattcgct tgccttttag
     2581 atcggcgatg cttttgatcg agtgattgcc ggctaaaaag aacgccgggc aattcaagac
     2641 gccgccgaca atcaccagat cgttactctc gagtagtgaa aagaccaaag agggagcgtt
     2701 gggcgttcct attggagtta caccggctac aatatcgtcg acgatcttct cttcatcctg
     2761 tcgattgacc agctcgacca agatattctg cttctcaaat agtcccgccg ccagcccgat
     2821 gaatggtaga aagcgcccga tactggaaac accgatgccg atcttgattt gatccattta
     2881 gctaagccac taccggttcc tgtaccggcg tatgactcgc cacgtccgga actcggcttc
     2941 gaggcacacc attgactccg gcatttccag ctccgagtag cagcaatctt ccctttccag
     3001 tgttacacaa ttgatacggc tgcttggatt ccagcatcac cgtgtcgtgc tgtttaagca
     3061 cgaattcctt gtgcggtgtg cgtacggtgc actcaccttc caggaccata aagatatgat
     3121 catcgttctc gtgataatgc atgtcgtctt ggattcccgg ttcgcagttg atcacccatg
     3181 cataggcgag cttggttctc cacaaactca aatgccggat ctccggatgg gcgtcagctt
     3241 cattgatcgg attcttcatc tccaccataa atcctccttt tcggttaact cgttccagcg
     3301 tttgcgatca ctcagatcgt gtaaagggca actcattcga gggcccgctt cggacgctct
     3361 ttgggcttca ctaaacgacc gtgggtgcca tcgcaataag gatggtcctt tgatctccaa
     3421 catcggcaaa tggcaatcgt tcttggttcg tcgaattcca gcatgcgcgg gctaatattt
     3481 gtgccggcat gagaaccatc gcaaaaaggc aaattcttgc tccgggtgca gcggcagtac
     3541 gcaacttctt tgctcacctg cttgacaatc accggttcaa tcaaatcttc gtagaatagt
     3601 cccactccag cccctttcgt ttatcgtgat tcgcgtcttt tgtgtccggc ttcttgatat
     3661 ggatttctga aaatgcgcgc aagggcaaac tatagaccca cttgagcttc gtcaaggttt
     3721 aaacattgta cccatccatg tatccacctg aggatctaaa ttgagtgcgg cgaatcttat
     3781 tggtgtttga gaccacccgg tgcttgggag caaccgcatc cgccagtggc gctgaattat
     3841 gctcagcagc acccggacac tatgccaggg ggtgaattcg tgcccgatag caaaatagta
     3901 aaggttgcgg ccgcgcttct acatctccat agctggctcg tttccggtgg ctcggcaacc
     3961 agtcgatcat cgggcgatcg tcttcggcgg cgacgtggag gcaatggcgt gggaaatgct
     4021 cccaagccaa actaaagaac aggttcgtcc gtcaatcacc gagttgttcg acggcagaga
     4081 agcggcttaa cgcaaaagaa tgacgctgag attccagaat cgatgacgca actggtgctg
     4141 ctatttcccg caatgatgca agagatgcgc agatgtgcag agttttccgt acgacccctt
     4201 gaatccccac agcaaacccg ttaggtaaat gcttttacgc gtatggccag gtagctcagt
     4261 cggtagagca gaggactgaa aatcctcgtg tcggcggttc gattccgtcc ctggccacct
     4321 ctgaccctca agtatttcaa acactgcgcc tcgacaaaga agcacatctc atccactttg
     4381 aagttcctga aattcgctgc tgatcaaacc tttgtaagtt ctagtttagc tcgggtcgtt
     4441 cccaatt
//
LOCUS       DASUVS010000181         3954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1603715, whole genome shotgun sequence.
ACCESSION   DASUVS010000181 DASUVS010000000
VERSION     DASUVS010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603715"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1122
                     /locus_tag="VF182_10075"
     CDS             118..1122
                     /locus_tag="VF182_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6437466.1"
                     /translation="MIRPSIFALLISLSISISAAAQDAKLIEQAKKEGGKLIVYGSLE
                     SFTVEPIAEAFQKKTGIAVEYWRASATKVMDRALSETRAGKALFDVMLNNSGAMHVLK
                     KEGMFIPYSSPATAAFPKEITDPQLGPIYRNTPIGIVYNTGAIKPADAPKSLEDLLHP
                     KYKGKFVMPDPTQHTTTLQWIASLYKIMGKEKAEKFTRELGATKPLLVESLAPAAERV
                     STAETPLGISLVRYVVTFSEKGAPVDYVRLGKMLSLGQYIALSSKASRPNSGKAFIDF
                     FLGDESMRMLAKQGEFVNRQGIYPSLPDADKIQAVEMDDFDAQGFKEKTREYQKIFLK
                     "
     gene            1405..1773
                     /locus_tag="VF182_10080"
     CDS             1405..1773
                     /locus_tag="VF182_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6437467.1"
                     /translation="MNAALKVSGIDHVVFHVKDLPRARKFYVDFLGLEVEHERDWQCF
                     LKCGNQGIALFEVDDGEEVHGGSEVNHMALRLDSGEYERVKATLEDAGIEVHGRKGDP
                     RCIYFHDPDGHQLQFLLPND"
     gene            complement(2163..3101)
                     /locus_tag="VF182_10085"
     CDS             complement(2163..3101)
                     /locus_tag="VF182_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6437468.1"
                     /translation="MFLRRHPSRLGIACTILLFDLILGSGALAQTPRGQDAKTITLGI
                     IADTNQKKVEANFQDFVRYVGRNLSASSGIQGKVMIAPTQSRLASLLTERRADFYMES
                     PYPTYLINDVYGAGKLLLRRWKGGMADYHSLIFTRTDGETKRLEDLKGKIIAFEDPQS
                     TSGYFLPKLFLTRKGFTLSRMGQIDAKVRPGEIGYVFATSQRKLFDLVLTKKVAAGAF
                     SNDDYAALEESKKPAITILAETASLPRHLVSVRKGLPPALVNGLEKVLLSMHRDSEGR
                     SIMQKAAGTTKFDALPGGESAISRRLLEIYYSPEKK"
     gene            complement(3486..>3954)
                     /locus_tag="VF182_10090"
     CDS             complement(3486..>3954)
                     /locus_tag="VF182_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437469.1"
                     /translation="GDEVLIRTGWGTRERAYQLGIDYYKRTPSIHFDAASILAKKMDE
                     MDSGFFMTDCGLVNPPRVQGNNWFRGESPLVPHPKPWPSAEARERVLDLGAHRHGSPH
                     PSSYGALIKKSIAGCKCLVDCDKITKPRVKMIILPLLIKQGGASPCRFIAVED"
BASE COUNT          953 a         1089 c         1039 g          873 t
ORIGIN      
        1 ataacgcgct cgatctgatc catatgtagg tcgatgaaac aatccaaacc ccagaccatg
       61 aacgcctaca tggctcgagc tgcggactcg tggtgaactt ctcatggaga gcttgtgatg
      121 attcgtccat cgatttttgc cctgttgatt agcctgtcga tttcgattag cgccgcagcg
      181 caggacgcca agctcatcga acaggccaag aaggaaggcg gtaaactgat cgtctacggc
      241 tcgttggagt catttaccgt cgagcctatt gccgaggctt ttcaaaagaa gaccggcatc
      301 gcggtggaat actggcgcgc ttcagccacc aaggtgatgg atcgggcgct gagcgaaacg
      361 cgcgccggca aggcgctgtt tgacgtaatg ttgaacaaca gcggcgcgat gcacgtgctc
      421 aaaaaagaag ggatgtttat cccgtacagt tctccggcaa ctgccgcttt ccccaaagaa
      481 atcaccgatc cgcaactggg cccgatctac cgcaatactc cgatcggtat cgtctacaat
      541 accggcgcga ttaaacccgc cgatgcgccc aagtcgctcg aggatctgct ccatcctaaa
      601 tacaagggca aattcgtcat gcccgacccg acgcaacaca cgacgacgct gcaatggatc
      661 gccagcctgt ataagataat gggtaaagag aaggcggaaa agttcacccg cgagctcggc
      721 gccaccaagc cgcttctggt ggagtctctg gcgcccgcgg ctgaacgggt atccacggcc
      781 gagactcccc tgggtatcag cttggtaaga tatgtcgtga ccttttcgga gaagggcgcg
      841 ccggtcgact acgtcaggct cggcaaaatg ctcagcctgg ggcaatacat cgcgctcagc
      901 agcaaggcgt cacgacctaa cagcggcaag gcattcatcg atttttttct cggcgatgag
      961 agtatgcgca tgctcgccaa acaaggagaa ttcgtaaatc gccaaggcat ctatccgtcc
     1021 ttacccgacg cggacaagat tcaagccgtg gagatggatg acttcgatgc tcagggattc
     1081 aaggaaaaaa cccgggagta ccagaagatt tttctgaaat agtaaaagtg gctagcccgg
     1141 attatccatg tttagaggaa aaaccacgac actatgtcga tcgaagggtg agcctggagg
     1201 agtgaagaca tctagacgaa cagcttcaat gaaccctttg gtgcttgatt ctctgctcgc
     1261 acttttccca ggcgtccgct ccacaactgt gttatgcagg ccgctccgag gcacaggccc
     1321 atttgtcttc gttccggagg gctcgcccgt cgctcaccgt caccagcgaa taatccgggc
     1381 taaatcattt tacggaggac gatcatgaac gcagctctca aagtaagcgg tatcgatcat
     1441 gtggtctttc acgtcaaaga tctcccgcgc gccaggaaat tttacgtcga tttcctcgga
     1501 ctagaagtcg agcatgagcg ggattggcag tgcttcctca aatgcggcaa ccagggcatc
     1561 gcgctctttg aagtcgatga cggcgaagaa gtgcacggcg gcagcgaggt caatcatatg
     1621 gcgctgcggc tcgactcggg cgaatacgaa cgggtcaaag cgacgctgga ggacgccggc
     1681 atcgaagtcc acggccgcaa gggcgatccg cgttgtatct acttccacga tcccgatggc
     1741 catcagctgc agttcttgct gcccaacgac taagggccgc tctttatcag atcgcgcgtt
     1801 ccaaccgttc caatagttcg agcgcatcgc tccgttcaag tcggcacacg gaagcgaact
     1861 cgctcaaccg ttcaacaatt caaatattgc agatcgcaac cgttcaacgg ttcaatagtt
     1921 caacagttca acgaaagaga ggaacttcca cgttcgagaa ttccggaaga tgagatcttc
     1981 ggacctacga gccaggtgag gtacaaagtc agcggggcgg ctaccgcttt actggattgt
     2041 aagaactggc ccaggtgaac tggcggcaac ttatatgtgc aataaagggg gcgcggaata
     2101 ggaagagccg agcacgggat cgagagccta tccgaagcgg cgagcccatt gctgactgac
     2161 ggctattttt tttccggaga gtagtagatt tccaagaggc ggcggctgat cgccgactct
     2221 ccacccggga gggcatcgaa ctttgtcgtg ccggcggctt tttgcataat cgatcggcct
     2281 tcggaatctc gatgcatcga cagcagaacc ttttccaagc cgttgaccag agcaggaggc
     2341 aaacctttcc gaacagagac caggtggcgc ggcaaactgg ccgtttccgc caggatcgtt
     2401 atcgctggtt ttttactttc ttccagtgcg gcataatcat cgttgctgaa agctcccgca
     2461 gcgacctttt tcgtgagaac caggtcaaac agttttcgct gagaagtagc gaaaacgtaa
     2521 ccgatttcgc ccggacgaac cttggcgtcg atttgaccca tgcgcgatag ggtaaatccc
     2581 ttccttgtaa gaaataactt cggtaggaaa tagcccgacg tcgattgggg atcttcaaaa
     2641 gcgatgatct ttccctttaa gtcttcgaga cgcttggtct caccgtccgt tcttgtgaaa
     2701 atcaaggagt ggtagtcggc catgccgcct ttccaacgcc tgagaagtaa ctttccggcc
     2761 ccatagacgt cattgatcaa atacgtcggg tagggactct ccatgtaaaa gtccgccctc
     2821 ctctccgtaa gcaggcttgc gagccgggac tgcgttggag cgatcatgac tttcccttgg
     2881 atccccgatg acgcggatag atttcttccg acatagcgga caaaatcttg gaaattcgcc
     2941 tcgacctttt tctgatttgt gtccgctatt attcccaagg taatcgtctt tgcgtcctgg
     3001 ccacgcggcg tctgcgccaa tgcgccggag cctagaatca gatcaaaaag gagaatggtg
     3061 caggctatgc ccaggcgaga tgggtgtctt cttaaaaaca taatgtttat cgaaaaccct
     3121 ctctcgcgta gatatgatac aaaccaaatt atcttgcaag gaaaaacagc gcacgccctt
     3181 caggctgtag acagtcgttc ccgtagtgcc acgcttcgct acgttcatcg tagaatcgag
     3241 ccttccaatc gttccaccgc atcgctccgt tcaagtcgta gaaaaatcga acttgttcaa
     3301 cggttcaaca gttcagtgtt gaacggcaga taatcaacga aactcgtcga aatggccgag
     3361 gttttaaatc ggttgaagcg tgaaccaagt tctacaaggc tctcaacagt acctgcaccc
     3421 ctctacccgc tgacggttca gcttcgatgc ttcaagcggg caacagaagc tcaaaacacg
     3481 acacctcaat cctccacggc gatgaaacgg cacggcgatg cgccgccctg cttgatcaac
     3541 agcggaagaa tgatcatttt cacccggggc ttggttatct tgtcgcaatc aaccaagcat
     3601 ttgcagccgg cgatcgattt cttaatcagc gcgccgtagc tgctcggatg cgggctgccg
     3661 tgccggtgag caccgagatc cagtacgcgt tcccgtgctt cggctgaagg ccacggcttg
     3721 gggtggggaa ccagaggact ctcgccacga aaccagttgt tgccttgcac cctcggcgga
     3781 ttcaccaggc cgcaatcggt cataaaaaac ccgctgtcca tctcgtccat tttcttcgcc
     3841 aatatggatg ccgcgtcgaa atggatgctc ggcgttcttt tgtagtaatc gatccccagt
     3901 tggtaggccc gctcgcgcgt tccccaaccg gtgcgaatca gcacctcatc gcct
//
LOCUS       DASUVS010000182         8886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_729828, whole genome shotgun sequence.
ACCESSION   DASUVS010000182 DASUVS010000000
VERSION     DASUVS010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8886
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729828"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VF182_10095"
     CDS             complement(<1..499)
                     /locus_tag="VF182_10095"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437470.1"
                     /translation="MLFIKNTPVDQILSMTEMIEAIEEALKELRLGRGFDLPRRRIHH
                     PNRMIFGLLPGSVDGIMGAYLQTDLDRRRHNETVILYSVETGEPLILFQNCSINEART
                     GAAGGIGAKYLARKDSRVAAVLGSQAHAVMQLQAAHAVRPLAAATVFSPTESHRRACV
                     EKIKRQ"
     gene            complement(591..1265)
                     /locus_tag="VF182_10100"
     CDS             complement(591..1265)
                     /locus_tag="VF182_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6437471.1"
                     /translation="MDFEVIVDNFRFLFVQGFLGFGEFAGGTLRLAIPAIVLGFALGI
                     VIGLGRIAKTPAVRLLATTYVEFFRGVPLVMVIFWFWFIIPNLLKISLPEYGIALTAF
                     VIFEAAYLGEIIRAGIQSVPRGQMEAAVATGLSRGSTMRHIVLPQALRNMIPALVTQF
                     IVLLKDTSLASIIGYIDLTKAAQIVNNREIRPFELYLFIAVVYWCFSYAMSRYARRLE
                     RQLNPA"
     gene            complement(1274..1993)
                     /locus_tag="VF182_10105"
     CDS             complement(1274..1993)
                     /locus_tag="VF182_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6437472.1"
                     /translation="MHYQFNWSVLWSGQSGAWLLQGVITTLQLSLLAWCLAVMLGIVA
                     GAFRTISFAPLRWLGSFYVEFFRNVPLLVWMFFWYFAGPPLLPQPAQDWLFDHSAEFW
                     AGMFALGVYHGARFSEVIRAGIQSIPRNQYESAVSTGLTLAQTYRLVIIPIALRLIIP
                     PATNETLNLLKNSSVALTIGVAELTFQTRQIETYTAKAIEALTAGTVIYLLLCLGIAV
                     LASRLERRFAIPGMIVQVKTD"
     gene            complement(2058..2915)
                     /locus_tag="VF182_10110"
     CDS             complement(2058..2915)
                     /locus_tag="VF182_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX6437473.1"
                     /translation="MRKISRAMLLVLLLTGVSGSAGAETTLEKIARTGTLTIGARTGS
                     PPFGYVNKNNEWVGFSIELVESAIAPVLSKKIGKKIKVEKKESAPQTRIPLLSSNAVD
                     LIAETMTDTQSRRESVDFSITYFITGAQFLVKRGNAINGLKATAGKRIAAQQGSTNAR
                     IIRERVPTAKLVEFPDQPAAFQALAQGQVQAYTNDGIQLAGLKAKAPKPDEWMVVGEF
                     FSYEPYGMAMRKNESDFRNVINVGLMDAIESGRYFEIYDKWLGQKGEVPYPVTAETKR
                     FLQMQVVPK"
     gene            complement(2924..3652)
                     /locus_tag="VF182_10115"
     CDS             complement(2924..3652)
                     /locus_tag="VF182_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009362713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX6437474.1"
                     /translation="MIEFHQVNKWFGSLHVLRNIDLNIEAGEVVVVCGPSGSGKSTLI
                     RCINRLETIQSGELTVDGLSLGDSNLDTAKLRANVGMVFQSFNLYPHMTALQNITLAP
                     VKVKGMGRSDAEKQARDLLERVGIPDKVSAYPANLSGGQQQRVAIARALAMRPKIMLF
                     DEPTSALDPEMIKEVLDVISALAREGMTMVVVTHEMGFARRVANRIVFMDEGNIVEIG
                     EPEKFYTAPQSPRAQQFLSKILSH"
     gene            3839..4297
                     /gene="sixA"
                     /locus_tag="VF182_10120"
     CDS             3839..4297
                     /gene="sixA"
                     /locus_tag="VF182_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEX6437475.1"
                     /translation="MEFYLVRHGEAASQPVNGDRPLTTSGRRDVERIGRAAAERGIKP
                     GQIFHSGLLRAQQTAEILSDSLDPAAGVRELVGLRPDDDPAVAKAELESSTISLMLVG
                     HLPHVSRLTSLLVTGDADRNLVEFAPATVIGLSCEDDRWTILWKLTATTA"
     gene            4376..5662
                     /locus_tag="VF182_10125"
     CDS             4376..5662
                     /locus_tag="VF182_10125"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX6437476.1"
                     /translation="MASDTLTITDNRTGKKYDIPVQNGAIKATDLFQIKVSEDDGLVS
                     YDPGFMNTASCQSKITYIDGDKGILRYRGYPIEQLAEQSTYLETAYLILYGELPGKSE
                     LADWIGNITIHSIIHENIKKHIDGFHHDAHPMGILVSTVAALSTFYPDAKKIFDAKSR
                     RDQTYRLISKMPTLAAFAYRHSLGLPYSYPDNDLSYTGNFLNMLFKMTELKYKPHPVL
                     EKALDVLFILHADHEQNCSTNAMRGVSSSQVDPYSSVAAAGAALYGPLHGGANEAVLR
                     MLMAIGSKEKVPDFMKKVKGGEGRLMGFGHRVYKNYDPRAKIIKIIADQVFEVRGRNP
                     LLDIALELERIALEDDYFIKRKLYPNVDFYSGLIYQSMGLPMDMFPVLFAIPRTSGWI
                     AQWEEMLTDPDQKLARPKQVYLGADKRDYVAMDRRT"
     gene            6289..6927
                     /gene="pssA"
                     /locus_tag="VF182_10130"
     CDS             6289..6927
                     /gene="pssA"
                     /locus_tag="VF182_10130"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX6437477.1"
                     /translation="MARTKSPNRTIHFSMLRSYQPADLITLANSAAGMAAILSIMSYL
                     QTPENWRVYLALSLIPAAFIFDILDGRLARRQHKNSVFGQELDSLADIVSFGVAPAAL
                     AYGLGMNGALDVVILIFFVSCGISRLARYNVTAAELADAGGKVRYFEGTPIPSSLILV
                     ALLALCFYLGHIGNDLPFGVVEIASFEWHPSSLVFFINGSAMISKTLKIPKL"
     gene            7164..8123
                     /locus_tag="VF182_10135"
     CDS             7164..8123
                     /locus_tag="VF182_10135"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HEX6437478.1"
                     /translation="MRICDLRVKIAGSELEPRIQRFFDELAAKGISFMPLCYLGDEWF
                     CPEGSAAIAIPFYLAHPRLKKLEERMMMEVEGGTETWCMRLLRHEMGHVLNHAYLIDK
                     DRGWQKLFGPPSLEYSESFRARPYSKRFVRHLAGWYAQSHPEEDFAETVAIWLTPGVD
                     WRQQYRDWTALAKLEYVDALMSKLAGEPPKVLSKVKMSEASRLRSRLEAYYKRRRRLY
                     AQDFPDFFDADLKKLFIDGSLAPTHERAAAFLRRSSNMLLNAVSAWTGEPKFTLNRVL
                     RALTQRCADLDLRLRSESAGVEVSAYLATLASHYRLTGKFKDT"
     gene            8130..>8886
                     /locus_tag="VF182_10140"
     CDS             8130..>8886
                     /locus_tag="VF182_10140"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437479.1"
                     /translation="MASAKRKYKVLVLFDTAGTPPSKQDFTEEMKTDDWAAEAHIIAA
                     LSQLGHDVRTIGVYDEPGMIVDEIKTYPPDVVFNLTEHFNAVSEHDRNVAGLLEMLDV
                     PYTGSSPTGLTLCKNKGMSKEILSYHDIRVPNFAIFPLGATLRRPKKLNFPLFIKPLK
                     EEASYGISQDSFVEDDTAFEERIRFIHERMNQEALAEEYIEGRELYASILGNDRLQTF
                     PLREVVFSAVPDGQPRFSTFKAKWDKAYRKRWGI"
BASE COUNT         2076 a         2432 c         2408 g         1970 t
ORIGIN      
        1 tctgtcgctt tattttctcg acgcaggcgc gccggtgaga ttccgtcggg ctaaaaacag
       61 ttgccgcggc caaaggtctg actgcgtgcg ccgcttgaag ctgcatcacc gcgtgagcct
      121 ggcttcccag aaccgccgcg acccgggagt ctttgcgcgc caggtatttg gcgccgattc
      181 cccccgcggc tccggtacga gcttcgttga tggaacagtt ctgaaacaga atcaaaggct
      241 cgccggtttc gacgctatag aggatgacgg tttcattgtg ccggcgtcga tccaagtcgg
      301 tctgcaaata ggcgcccatg ataccatcaa ccgagcccgg cagcagtccg aaaatcatgc
      361 gattgggatg gtgaatacgc ctgcgcggca ggtcaaagcc gcgccccaag cggagttctt
      421 tcagggcctc ttcgatggct tcgatcattt ccgtcatgct gagaatctga tctaccggcg
      481 tgtttttgat aaaaagcatc gcacctccaa agtggtctca tccgatatga gcctgaacga
      541 ttcgaatcga ctgtgaactc ggattggaaa agcggcaacg atcgcgcggt tcacgccgga
      601 ttcagctggc gttcaaggcg ccgggcgtag cgcgacatcg catagctgaa acaccagtaa
      661 acgacggcaa taaacaggta cagctcaaac ggtcggatct cccggttgtt gacgatctgg
      721 gccgctttgg tgagatcgat gtagccgatg atcgatgcca gtgacgtgtc tttgagaaga
      781 acgataaatt gggtaaccag agcgggaatc atattgcgca acgcctgcgg cagcacgata
      841 tggcgcatcg tggagccgcg ggagagcccg gttgccaccg ccgcttccat ctgccctcgg
      901 ggaaccgatt ggatgccggc gcggatgatc tcgcctagat aggccgcttc aaaaatgacg
      961 aaggcggtaa gcgcgatgcc atactcaggc aacgagattt ttaaaagatt ggggatgata
     1021 aaccagaacc agaaaatcac catcaccagc ggcacgccgc gaaagaattc gacataagtc
     1081 gtcgcgagca accggacagc cggcgttttg gcgattctcc caagaccgat gacaataccc
     1141 agtgcgaagc caagcacaat ggctggtatt gccaggcgca gcgttcctcc ggcaaactcg
     1201 ccgaacccta aaaacccctg gacgaaaagg aaccggaaat tatcaacgat gacttcaaag
     1261 tccatggaca actctaatcg gttttgacct gaacgatcat gccgggaata gcgaagcgcc
     1321 tttcaagacg tgatgccaga actgcgatgc cgaggcagag taataaatag atcactgtgc
     1381 cggcagtcag cgcttcgatc gccttggcag tgtaggtctc gatctgccgt gtctgaaatg
     1441 tcagttcggc aacgccgatg gtcaacgcca ccgatgaatt tttgaggagg ttcaatgttt
     1501 cgttggtggc gggcggaata atcaatcgta gtgcgatggg aatgataacc aggcggtatg
     1561 tctgtgcgag ggtcaaaccg gtggaaaccg cagactcgta ctggtttcgc gggatcgatt
     1621 ggattccggc ccgaatgact tcggaaaaac gcgcgccgtg atagaccccg agcgcgaaca
     1681 tgccggccca gaattccgcg ctgtggtcga acagccaatc ctgcgcgggt tgaggcaata
     1741 gcggtggacc ggcgaaatac cagaagaaca tccagaccag cagcgggaca ttgcggaaaa
     1801 attccacgta gaacgagcct agccagcgta gcggcgcgaa tgagattgtg cgaaacgcac
     1861 cggcaacgat gccgagcatg accgcaagac accacgccag caggcttagc tgaagggtcg
     1921 tgatcacgcc ctgcagcagc caagcgccgg attggccgct ccataagacg ctccaattaa
     1981 actggtagtg catgcgcgag gcaggtgcgg agccaggata gaactctatc tatatcctgg
     2041 ctaacccggc acctcaatca tttgggcacc acttgcatct gcaggaagcg tttggtttcc
     2101 gcggtcacag gatacgggac ctcgcctttt tgcccaagcc acttatcgta aatttcgaaa
     2161 tagcggcccg attcgatagc atccatcaat ccaacattga tcacattccg aaaatcggac
     2221 tcgttttttc tcatggccat gccatagggc tcataagaaa aaaattcacc gaccaccatc
     2281 cattcgtccg gcttcggtgc tttggccttc agtccggcga gttggatacc gtcgttggtg
     2341 taggcttgca cttggccctg cgccagcgct tgaaaagccg ccggttgatc cggaaattcc
     2401 accagcttgg ctgtgggaac gcgttcccgg ataatgcgcg cgttggtcga accttgctgc
     2461 gccgcaattc gtttgccggc ggtggccttc aggccattga tggcatttcc tctcttgacc
     2521 agaaactgcg ccccggtgat aaaataggta atgctaaaat ccacgctctc ccggcgtgat
     2581 tgcgtatccg tcatcgtctc cgcaatcaag tccacggcat tggaggagag cagcggaatg
     2641 cgggtttgcg gtgctgattc tttcttttcg actttgattt ttttgccgat tttcttggac
     2701 aacaccggcg caatcgcgct ttcaacgagc tcgatagaaa acccgaccca ctcattattc
     2761 ttgttaacat agccgaaggg cggcgatccc gtgcgagcgc caatcgtcag ggtgcccgtg
     2821 cgtgcgatct tctccaaagt tgtttcggct ccggccgatc cgctcacacc agtcagcagg
     2881 aggacaagga gcatagcgcg ggaaattttt ctcatattga ctcctaatgc gagagaattt
     2941 tgctcagaaa ttgttgcgcg cgcggcgatt gcggcgccgt atagaacttc tccggctcgc
     3001 caatctcgac aatgtttcct tcgtccataa atacgatgcg attcgcgacg cggcgcgcga
     3061 agcccatttc atgcgttacg accaccatcg tcatgccctc ccgggccaga gcgctgatca
     3121 cgtccaagac ctctttgatc atttccgggt ccaaggcgga ggtaggctca tcgaagagca
     3181 tgattttggg acgcatggca agcgcgcgag caatggcaac ccgctgctgc tgaccgcctg
     3241 agagattggc aggataagcg cttactttat cgggaatgcc gacccgttcg agcagatctc
     3301 tagcctgttt ttcagcatcc gagcggccca ttcctttgac tttgaccggc gccagagtga
     3361 tattctgcag cgccgtcatg tgcggataga gattgaaaga ttgaaaaacc atgccgacgt
     3421 tggcgcgcaa ctttgcggta tcgagattgg aatcgcccaa agaaagcccg tcaaccgtca
     3481 gttcgcccga ctggatcgtt tccagccggt tgatgcaccg gataagagtg ctcttgccgc
     3541 tgccgctggg accgcaaacg acgacgactt ctcccgcttc gatgttgaga tcgatatttc
     3601 tcaacacgtg cagcgatcca aaccatttgt tgacttgatg aaattcgatc acgttacgag
     3661 gtaaaccaaa aagcaaaata gaattgttga accccggcat cctatatcaa catgtgcttt
     3721 ttgcaaccgg atctcatgcg gctcgaaata ctggaattcc gatcgctatc gagcgttcac
     3781 agagatctta tccgcgacat attgctgtgt ccggcagcgg tgttaagtta agtcgacgat
     3841 ggaattctat ttagtccgtc acggcgaagc agcttcacag cccgttaacg gtgaccgtcc
     3901 actgacgacg agcggtcggc gcgacgtgga acgcatcggc cgggcggcgg cggaacgtgg
     3961 gattaaacca ggccaaattt ttcactcagg gcttctccgg gctcagcaaa cggcggaaat
     4021 cctgagtgac agtctcgacc ccgcggcagg agtacgtgaa ctcgtcgggt tgaggcccga
     4081 tgacgatcca gccgtcgcca aggccgaact cgagtcgtcg accatttcgc tgatgctcgt
     4141 tggtcacttg ccacacgtca gccggctaac ttctcttctg gttaccggcg atgccgatcg
     4201 gaacttggtc gagttcgctc ccgcaaccgt gatcggttta tcgtgcgagg atgatcgctg
     4261 gacaatcctt tggaaactca cggctacaac ggcgtagagc aagttgaccg gcgtaagagg
     4321 atagtctaaa agggatcgat aacaagaacc tgggtgaaat tcgaaaggta gcgcaatggc
     4381 ttcagacact ctgacgatta ccgataatcg aaccgggaag aaatacgata tcccggttca
     4441 aaatggcgcg atcaaggcca ccgatctgtt ccagattaaa gtctctgagg atgatgggct
     4501 ggtcagctac gaccccggtt tcatgaatac cgcgtcatgc caaagcaaaa tcacctatat
     4561 cgatggtgat aaaggaatct tgcgttatcg cggctatcct atcgaacagc tagccgagca
     4621 gagcacttac ctcgaaactg cttacttgat tctctatggt gaattgcccg gcaaatccga
     4681 gctcgcggac tggattggca acattactat tcattccatc attcacgaga atataaaaaa
     4741 gcacatagac gggtttcatc atgatgcgca tcccatggga atcctggtca gcaccgttgc
     4801 agcgctatcg actttttatc ccgacgccaa gaaaatcttc gatgctaaat cgcgccgcga
     4861 ccaaacctat cgactgatca gcaagatgcc gactttggcc gcctttgcct accgtcacag
     4921 cctgggcttg ccttactctt atcccgacaa cgatttgagc tacactggga attttctcaa
     4981 catgctgttt aaaatgaccg agctcaaata taagccgcac ccggtgttgg agaaagccct
     5041 cgacgttctt tttattctgc acgcggatca cgagcagaat tgcagcacca atgccatgcg
     5101 cggggtctcg agctcgcaag tggatccata ttcttcggta gcggccgccg gcgccgcgtt
     5161 gtacggaccc ctgcatggcg gcgctaacga ggcggtatta cgcatgctca tggcgatcgg
     5221 ctccaaagaa aaagtgccgg attttatgaa aaaggttaaa ggcggcgagg gccggctgat
     5281 gggctttggg catcgggtat acaagaatta cgacccccgc gcgaaaatca tcaagatcat
     5341 tgccgaccag gtcttcgaag tcagaggacg taatcctctt ttggatattg ctcttgaact
     5401 cgaacgcatc gcactcgaag atgattactt catcaaacga aaactctacc ccaatgtcga
     5461 tttctattcc ggcctaattt atcagtcgat gggtctgccc atggacatgt ttcccgtgct
     5521 atttgcgatc ccacgcacct cgggctggat cgcgcaatgg gaagaaatgt tgacggatcc
     5581 ggaccagaag ctggcccgtc caaaacaggt ctatctcggc gcagataaac gcgattacgt
     5641 ggcgatggac cgacgaactt agttggcgag cacagttccg acgccgtttg ggatcccgtg
     5701 cgctcaacca atcatgcttg cgttcagcgc taacgcagcg tgccgcgcaa gctctggagc
     5761 agtaccctta gcttctgtcc gacccgtaaa ctagagcctt gcaagccgtt gaattctacc
     5821 aaaagccgaa cttctcgccc gaagcggcgt gcgatcgaga gcagattctc gcctcgtcga
     5881 acctcgtgaa gtaggagtta ggctatttgc atccgttatt cagggtgctt ctcctccgct
     5941 cgcattcccc aatgacaacg tgacacgaaa cgattttctc cccggagaag tagtgctttg
     6001 cagcaactcg gggattcaac tgtctaatcg tttgtgccgg agtgccggtg atttcggcgt
     6061 ctatcggcag atggtaatcg ctcaagtcga atccatgctt ttgtggcttg ggcgcgagcc
     6121 cgtgcctgcg cagcaggtat gaccatatac acagcggatc gtaagactta catgatccat
     6181 tacaatggag tactacatga tccgggctac cagtgcacga cacggcgtag acgtctgcgg
     6241 atagggaatg tatcttcggc cagcgcacgg aaccggaggt aaagacacgt ggcaagaaca
     6301 aaatcaccca accgaacgat ccatttctcg atgctgcgga gctatcaacc cgcggacctg
     6361 attacgcttg cgaacagcgc ggcgggcatg gcggcaattc tctcgatcat gagctatctc
     6421 cagacacccg agaactggcg cgtgtacctg gcgctatcgc taattcccgc cgcatttatc
     6481 ttcgatatct tggacggcag gctagcgcgc cggcagcaca agaattccgt gttcggtcag
     6541 gaactcgact ctttggcgga catcgtcagc tttggcgttg cgccagccgc gctggcttat
     6601 gggctgggaa tgaacggagc tctcgatgtg gtgatactca tattttttgt cagctgcgga
     6661 ataagccgtc tggctcgata caacgtcacc gccgcagaat tggccgatgc cggaggcaag
     6721 gtgcgctatt ttgaagggac gcccatccca tcgagcttga tcctcgttgc cttgcttgcc
     6781 ctatgcttct atctgggtca catcggcaac gacttaccgt tcggcgttgt cgagattgcc
     6841 tcgtttgagt ggcatccgtc gtcgctggta tttttcatca acggcagcgc tatgatcagc
     6901 aagacgctca agattcccaa gctatagggc cgtgttcacg ggttgagcag gatgaggata
     6961 ggcttgatca atgcagcggc ggatttcagc cccgaggcag ctcaagttcc atcggaccgg
     7021 acacaaacat atgggcgctc ggatcgttct ctgttaaact gactagaatg gaggatgcga
     7081 ataagcacaa accgaccgcg ccagctcatg ctccgtttgc cgcacccgac ctctccggaa
     7141 agtctgatga cgagctcttg cagatgcgca tctgcgactt gagggtgaaa atcgccggca
     7201 gtgaactgga accccgcatt caaagattct ttgacgagtt ggcggccaaa gggatttcat
     7261 tcatgccgct ttgttacctg ggagatgaat ggttttgtcc cgaaggatcc gcagccatcg
     7321 cgataccgtt ttatctggct catccgcgcc tgaagaagct cgaagagcgc atgatgatgg
     7381 aagtcgaagg aggcacagaa acctggtgca tgcggctctt gcggcatgag atgggccatg
     7441 tgctgaatca tgcgtatctg atcgacaaag atcgcggctg gcagaagctc ttcgggccgc
     7501 cgtccttgga atactccgaa agctttcgcg cgcggcctta cagtaaacga ttcgtccggc
     7561 atctcgccgg ttggtacgcg cagagccacc ccgaagaaga cttcgccgag accgtcgcta
     7621 tctggcttac gcccggggta gattggcggc agcagtatcg cgattggacc gcactggcta
     7681 agttggagta cgtggacgca ttgatgagca aactcgccgg agaaccgcct aaggtgcttt
     7741 ctaaagtaaa gatgagcgaa gcctcccggt tgcgctcgcg cctggaggcc tattacaagc
     7801 gccgccggcg cctttacgcc caagattttc ctgatttctt tgacgccgat ctaaagaagc
     7861 tttttatcga cgggagcttg gcgccgactc acgagcgcgc cgctgcattt ctgcgacgtt
     7921 cgagcaacat gctcctgaat gccgtgtcgg cctggaccgg agaaccgaag tttaccttga
     7981 atcgcgtgct gcgcgcgctc acacaacgtt gcgcggatct cgatcttcgc ctgcgcagcg
     8041 aatccgccgg cgtcgaggtc agcgcctatc tggcaacttt ggcatctcac tatcgcttga
     8101 caggtaaatt caaagatacg taaggagtca tggcgtcggc taagcgcaaa tacaaagtcc
     8161 tagtgctctt cgacaccgcc ggaacaccgc cgtcgaagca ggacttcact gaggaaatga
     8221 aaaccgatga ctgggcggcg gaggcgcata tcatcgccgc gctcagccag cttggccacg
     8281 atgtgcggac catcggtgtt tatgatgaac ctggaatgat cgtcgacgag ataaaaactt
     8341 atccgcccga cgtcgtgttc aatctaacgg aacattttaa cgcggtatcg gaacatgacc
     8401 gcaacgtggc cggtctactc gagatgttgg acgtccccta taccggctca agtcccaccg
     8461 gactgaccct gtgcaagaac aagggcatgt ccaaggaaat cctctcctat cacgatattc
     8521 gcgtgcccaa tttcgcgatc ttcccgcttg gcgcgacact gcgccggcca aaaaaactca
     8581 acttcccgct ttttattaaa ccgttgaaag aggaggcttc ctacggcatt tcgcaagatt
     8641 cgttcgtcga ggacgatact gcttttgagg aacggatccg cttcattcat gaacgcatga
     8701 accaggaagc tttggccgaa gagtacatcg agggtcgaga actgtatgcg agtattctcg
     8761 gcaacgatcg tttacaaacc tttccgctgc gtgaagtggt tttttcggca gtccccgatg
     8821 gccagccacg cttctcaacc ttcaaagcaa agtgggacaa agcgtatcgc aagcgctggg
     8881 gcatcg
//
LOCUS       DASUVS010000183        29559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1823221, whole genome shotgun sequence.
ACCESSION   DASUVS010000183 DASUVS010000000
VERSION     DASUVS010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 29559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29559
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823221"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1555)
                     /locus_tag="VF182_10145"
     CDS             complement(<1..1555)
                     /locus_tag="VF182_10145"
                     /inference="COORDINATES: protein motif:HMM:NF004780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX6437480.1"
                     /translation="MTTTEMSPDSHTRIPVLIVGAGPVGLSLALELGWRGIRCLLVEP
                     NEHFSGNPKAKTVHQRTLELFRRWGRGIPDKLRAAAPLGADFPSTILYVTRLTGQLIT
                     ALHNATPADSGNRFSPERTLRIPQSFLEPVLREEVREMPGVILRLGWKLEGLEQRRGE
                     VRAEIVHVESGARQEITAEYVAGCDGGRSTVRKLLGIRLDHWPGLKHALGGVFRAPTL
                     WSTLAFEKAFHYNVLNDDLPHLAVVGPLHPPDAWFFDLMGVDPDRLDPAEVIRNIVGQ
                     PIPFELQHVAPWTVHNALAERYHQGRVFLLGDAAHLQSPSGGYGMNGGVGDAVNFGWK
                     LAAVLQGWGGEDLLETYEIERRQFHIRALEESIQNSEDNELLTPRLEDPVHGATVRQT
                     LAGRIQLTKPKNFQSLGVSLGYRYSTSPVILPDGTPPTPYETSRYIPTAQPGHRAPHT
                     WLPDGTALFDHFGQGFTLLKLGHSEIDCSSLERAACNRGVPLTVLRRSEPELRELYQG
                     RLVLIRPDQH"
     gene            complement(1645..2421)
                     /locus_tag="VF182_10150"
     CDS             complement(1645..2421)
                     /locus_tag="VF182_10150"
                     /EC_number="4.1.3.34"
                     /inference="COORDINATES: protein motif:HMM:NF004868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEX6437481.1"
                     /translation="MAETEKLVSSMGKAELDKIFVRGYDLNEQLLGKISFAQMVYLMI
                     AGRMPTAEEGRMIDTMLIVLVDHGMTTGAAAARMTFHSAPEAIQGAVAAAILGAGSVH
                     LGSSEHCAKMLHDALPPDSKETDLDAIAQAAVEKRLANKQVVPGIGHGIHTGGDPRAE
                     RLFQVAQETKVYGRYCELLRKIGKVADQRVGRHLPVNVTGAIAAISMDMGLHWQMSKS
                     FAILGRALGGLAHVGEEIRRPIARGISNLIRDNLQYEPEK"
     gene            complement(2506..3609)
                     /locus_tag="VF182_10155"
     CDS             complement(2506..3609)
                     /locus_tag="VF182_10155"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6437482.1"
                     /translation="MSEQAMRVIDIDGHVREADDLWARYLEQPFRARAPKIERVANGQ
                     LLFLLEGKRHHRKPDETPFREKNDGSPVNEHRDLAIDPRARLKDMDRDGIERGLLFPS
                     AGLYLTSVEEEAYATALCRAYNNWLYDYCGADRGRLMAVGAIPVQDVNAAVAETRRVV
                     NELGFKAIFARPNPVKGRTLDDPYYDPLYDMLQELGVPLMVHEGSGSYLPTAGVDRFA
                     GQWFFTHTISHPFEQMLASLGLICKGTLERHPKLQVVFLEAGAGWLPYWLWRMDEHYE
                     ILPFQVPWLKMKPSDYFRRQCFTSCEPDETRLGEVIESIGAERVLFASDYPHWDAKLS
                     GSPQAVIDRPDIHADDKRKIMGENAARLLRLDS"
     gene            complement(3612..4562)
                     /locus_tag="VF182_10160"
     CDS             complement(3612..4562)
                     /locus_tag="VF182_10160"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437483.1"
                     /translation="MKRTSAALTWASLILVIVAVVPAWCAEKSLEKVRIAVSSKSLGF
                     LDVWAAREGGFYRNHGIEAEIIAMRPPLTIGAIQAGEIDYAFGASSISRGSMSGAPVK
                     IVSLSLRTSFHTLVARSSIKSMADMKGKTIAVTIGAADDFVARLLVRRGGLDVRDVSF
                     VNMGGSDTRFPALATGRIDATALSLPFFVIARRQGYNLLGSAGDVIDMATVGIGTSTR
                     KIQQEREQVRKMVRAQLDTLHWIKNQKAEVVPFLQKYFDLEPDVATESHAIYSRLIVD
                     DAKPLAEAIKTVLEQQGKPDLPLDRVVDSSIVEEVLRERR"
     gene            4814..6061
                     /locus_tag="VF182_10165"
     CDS             4814..6061
                     /locus_tag="VF182_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX6437484.1"
                     /translation="MPNYAAKLTFGPEAADWQERFNPDRMRRQRVERAQIMMRKYGVA
                     ALLEANHHNIRYLTALKGFAYPMCRYVLFFAEHDPVMYEHNGYYHQMPDQCPWIKEWR
                     PARSWLTGSPGPEACAAEAKAFAAEIKTELEKRGLLGEKLGLGSFDGNAREALVNAGV
                     KNIVDTRQLLLEARMVKNQDEISCLKTCAAMVDGVWYRIWEILKPGMRDTDLMTIGAS
                     AGYQIGLEAAVPGGWRSGPMTFDRGCHATNRIIQVGDLVYGSYCGATYMGYGSCTYRT
                     FMVGRNPTTKEKDWYKQVRERLDAVIEEIKPGKTTADAAKHFPPASKWGYKSEVEVLA
                     SEIGHGIGLGTSTGYDAPIINRLWSFEHPQLLEEGMTIAVESREGETRVGGARLENML
                     VVTKNGAEIMDYFPRDEILVAPR"
     gene            6109..6798
                     /locus_tag="VF182_10170"
     CDS             6109..6798
                     /locus_tag="VF182_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6437485.1"
                     /translation="MSQLRCLTIKAQEPSRVADFYRSVFELKTVTEDPELVRLSDGVF
                     TLAVLKESKEDSTGLHASGFQVEDLGKVAQKSEKGGQALPQSEAQVADPDQNLIDLTT
                     NDFGLAPEKTRYPIRHIALYTADPKRLSNFYGDAFGMKEVAHSDRSSIFVSDGYFNLA
                     LLYQRVREEKNGFNHFGFRVDDLEQIRDRAEKAGTRRGAARPSRIPFAEVRLHDPEGN
                     GVDISVKGWDV"
     gene            6911..8113
                     /locus_tag="VF182_10175"
     CDS             6911..8113
                     /locus_tag="VF182_10175"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6437486.1"
                     /translation="MPIQALQHVTVIECATFVTGPYASALLANLGARVIKIESPPRGD
                     PYRYFAPDPHYSFNFAHLNRNKQSLVLDLKTARGRKICSELLKSADVFVENFRPGTVE
                     RLGLGYETLRALNPRLVYCSISAFGQSGPYVDKPGFDTLGQAVSGLLSLLNDSDDPKV
                     MGMALSDYITGMSAGYGILGALLAREQSGEGCRVETSLLQATLSFIGESAAGYLRTGS
                     VPNRMARVKNAHAFAFICGDGLPLAIHCSVPEKFWLALLEATGRMDIAVDQRFATRDA
                     RRQNYACLEETLASTFKTKSRKEWLRCLEACDVPVVPLHDIAEALADPQIRHLRLIET
                     VEHPIAGPMEFVGAPVRYRNFTESPSLPPPLLGEHTNAILRELGYSDQAIEEVRAEQV
                     IGIADQAP"
     gene            8737..9735
                     /locus_tag="VF182_10180"
     CDS             8737..9735
                     /locus_tag="VF182_10180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6437487.1"
                     /translation="MIYDVEINSAAHYPAAEVPGLVVLAEQVGFGAFWKGESNSTDPV
                     VMLSAVASRTKTIKLGTAIYHIYGRSPVTLGIQAATLQDLSGGRLLLGLGVANKSIAG
                     WHGGVFDRPLKRAREYIDIVRKVAAGERVEYEGEIYQTGKRFQLSWKPAHQNVPVYLA
                     GLGPQMTKLVGKISDGVFINMATPAKVKEIAARVREGAVEAGRDPSKIEIIAKSRVSL
                     NPDKQAARNKLRQVLTFYNIADHYSDMLRGMGFEKEVNAIHEAFQKGGFKAAMGALSD
                     DYMDKLPVVPASDVKEIKEKMKEFEEAGVTRMVIPYVPVTEPVEDARRFLEAWGKA"
     gene            9861..10883
                     /locus_tag="VF182_10185"
     CDS             9861..10883
                     /locus_tag="VF182_10185"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HEX6437488.1"
                     /translation="MCDFAADAAAAEQLMVGRTLSVERVRQLNAKDYFYQMLKDNPEM
                     LKIYPGIENELLEGAIDCHIHAFPDFVHRSQDMIQIAIEASKCGMRAIAFKDHWNISA
                     TSAYLTQRYIDDMIARGELSHRVEVYGGVGMCFGMRPEYVRVGLQYPNFKMIWFPTFT
                     SYGFWRGAGHPEKEGVRLVAEDGKVLPEVKEIMEMAAEKRVGIGFGHTDFQELLPLAR
                     LAKDIGVRATLDHPLLELNKLLLDEMKQLRDLGVYVGTYCQPMIPSLYQPVADPMETI
                     RTIKEIGPDRCIIGSDFGQVLHMDAVDGMRVFIRALLGFGIKPDEVKVMLKENPAKLM
                     WLDEPR"
     gene            complement(11883..12485)
                     /locus_tag="VF182_10190"
     CDS             complement(11883..12485)
                     /locus_tag="VF182_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018304498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX6437489.1"
                     /translation="MSDWITVFENMGTVGVALLMFAENVFPPIPSELIMPLAGFSAAQ
                     GERNLLIVIIAGSIGSLLGAVLWYYVGQKIGAERLERWAAKHGRWLTLSPNEVEQACA
                     WFNRHGGKAVFIGRLIPAVRTLISVPAGIAGMPLGSFLLYSAAGTILWTALLGTAGYF
                     LESQYEKVAHWMNPISNVVIGLIILGYLYRVVSFQRRRAG"
     gene            complement(12536..13018)
                     /locus_tag="VF182_10195"
     CDS             complement(12536..13018)
                     /locus_tag="VF182_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
                     /protein_id="HEX6437490.1"
                     /translation="MRRSITGFNLDEYGDWVAFLSCGHKQHVRHRPPFEERPWVISKL
                     GRSKKLGKPLDCIRCDRFEMPDNFKVYRQTGEFNERTIPRSLRRDHSTKPGVWAKIQV
                     IEGELNYRIDALGVQFELSPERPGIVIPEIRHHVEPIGGVRFFVEFYKKAESDRDDDT
                     "
     gene            complement(13194..13649)
                     /locus_tag="VF182_10200"
     CDS             complement(13194..13649)
                     /locus_tag="VF182_10200"
                     /inference="COORDINATES: protein motif:HMM:NF025032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEX6437491.1"
                     /translation="MEQSFGRISMSGFLLLMSLFGCSTRESVSLPSASSPLVGRWRQT
                     SIATGNQSAKCPATMPLPGGSTTSCSGNDMIEFDPNGTFTATFSGSSVKGSGTWRLTG
                     NNLALTFTAPPNVAGRSQSATIRFGEGGKTININSKLGETRTTETYTRQ"
     gene            13818..14261
                     /locus_tag="VF182_10205"
     CDS             13818..14261
                     /locus_tag="VF182_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437492.1"
                     /translation="MRTTWLLIACALMSSASLGEGAEYFIYRDAAGRIVLANETPPPG
                     AEIVKRHELQDVSDEAVRAAREHEEFFWRRLKDEQLAESNQRLAESKDRLAEAIITAA
                     ALRETEPEVLIQVASSHDSRFRGLRLDQGRIPARPPAGFRHTKIR"
     gene            complement(14258..15973)
                     /gene="ybaL"
                     /locus_tag="VF182_10210"
     CDS             complement(14258..15973)
                     /gene="ybaL"
                     /locus_tag="VF182_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HEX6437493.1"
                     /translation="MPHSVTLISTIAAGLGLALVMGFIAARLRLPVLVGYLTAGMIIG
                     PATPGFVADVELSSQLAEIGVMLLMFGVGLHFSLDDLLAVRRIALPGAVVQITVATLL
                     GMAVSATWGWSLAAGLVFGLALSVASTVVLLKALEHRGVLDSVNGRIAIGWLIVEDLV
                     MVLVLVLLPAISVWLGGATESAGDRSLLKAFALTVGKVSVFIVLMLVAGRRLFPWLLW
                     QIARTGSRELFTLCIVAAAVGIAYGSAELFGVSFALGAFFAGMVMRESPLSYRAAEES
                     LPLRDAFSVLFFVSVGMLFEPDVLISEPLHVLMVLAIIIFGKSLAAFFIVLLFRYPLN
                     TALTVSASLAQIGEFSFILVGLGVSLGLLPTEGQSLILSGAIISIALNPLLFGAIEPA
                     QAWIRSRSKLAQTLERPDDPLAALPMTTDLKRLTGHVVIVGYGRVGRRIGEALTAHGI
                     SIVVAEENREIVERLRSSGIAAVSGDASDPAVLLQAHVHRASILVIATPDTLNVRKMI
                     EIARTVNPHIQTVVRTHSEEEAVLLEKEQAGKVFLGEHELAIGMIQHVLECHAARVEH
                     DKRQA"
     gene            16586..17809
                     /locus_tag="VF182_10215"
     CDS             16586..17809
                     /locus_tag="VF182_10215"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6437494.1"
                     /translation="MARKWWLVIVGCITHAVNTGLSYFGMSAYFPSFEREFGWSRTAI
                     SGAFSLARIESGLLGPMEGYVTDRVGPQRMIFIGLFLCAGGFLALSLVDSLPMLYAVI
                     VLGIVLGSSLGYNMPISVLIAKVFRERRSLAFGIFRMGPGISGPMVPLVGWMIGLYGW
                     RTAAVISGCVLLAVGLPLACIIRKIYSQEEAAILTAEHSGADLKHSPHASTEPQFTLK
                     QALRHRSFWLLSVAMGMRHLVTEGVSVHFVILLVDRGWSTEAGSTLLGISALVGAPAR
                     IGMGWLGDLLDKRRLVMGLLLALSISVLLMGYTAQAFIFTTCMIVYSLAYGGLAALQE
                     PIRADYFGTRAFATIQGMSRSVVTAGTFLGPIIAGFFYDVTKSYTIAFGVFAVVSLVA
                     TLFMFLAKPPRGCAD"
     gene            17974..19038
                     /locus_tag="VF182_10220"
     CDS             17974..19038
                     /locus_tag="VF182_10220"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6437495.1"
                     /translation="MRQNRFPVIDGDGHVTERNKEILEFLGGNYRGTSKDQSWADTFS
                     PFPSLDGWARLSGMSSPGLRDYPDCDTWISFLDDCGIQTTIVYPTAALAFGLIQDPAW
                     AVDIARAYNSWLHASYTKRTSRILGAALIPIHDVPAAVRELRRVKEELGMPAAMLPSV
                     TNLRKGYGHESFFPIYEEACRLDMPLAVHGAPSRGLGFDFFDSFAEVHALEHPLPLMI
                     QLTHMIFQGVFELYPDLRVAYLEAGCGWIPFMMDRLDEDFERRGKKWAPRLKRAPSEY
                     IKGGSVFVSLESEERTLPYVLELFGEDHIFFASDYPHERQRSQYLEDIPGIEARADIS
                     DSAKQKILAGNAKRFYRLSD"
     gene            19067..20110
                     /locus_tag="VF182_10225"
     CDS             19067..20110
                     /locus_tag="VF182_10225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX6437496.1"
                     /translation="MHLTTIGVRILLASFFLFLPQWQTGASAAAKLQCGPTTSTSNDL
                     PRTVAIASNLAGTGAHALASGLAAVASRSTPIAAKVQPYNGPNAWMPLMENGEIEFGI
                     INILDSHMAVTGTGNYKKPYPILRVVSGGVFPFTGSIMVRDKSEIKQPSDLKGKRMAW
                     DFGGHAITQTWQNAAMEVSGLKPSDVVQVRFSNLNDAIRAVPEGRVDATFAAIGIGIN
                     EEVNAMEPIRFLSLPNTDASNKILSKYGGSIVKQDPTAGIRGDTYVIGYPLHLASSTK
                     VNDKTVATVLKAWWDNLPELQTIHPQFKKWTKDVQAITNFTIPYHSGAVKFYKDAGVW
                     TEKHDLRTKEICS"
     gene            20367..22334
                     /locus_tag="VF182_10230"
     CDS             20367..22334
                     /locus_tag="VF182_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEX6437497.1"
                     /translation="MEIEENVRSRELSGLAGWITQALLIAIPVSGMFFLLDVPQRLDW
                     QVFHEQYIGLFLALTLAAIFLLIPGSKGQSQRIPWFDYIGAAAGLFVGLYIFVKFPEI
                     SNSLGDIYTERVILGAITVILLAEACRRLVGWPLVIIAGVFIFYAFFAYLFPGDFYGR
                     GWSIGRLGTYLYLDANGIIGQPLQIGASIVLVFVLFGEALYAIGGAAFLSDFALAVMG
                     RFRGGQAKIAIVSSSLFGNISGSAVANVVVDGAFTIPMMKKAGYPSPVAAAIEAVAST
                     GGQIMPPVMGAAAFLIAEYLAIPYAQVALAAFVPALLYYIALFIQVDLLAARNGIYGL
                     PPAELPRLLPVLRRSAGFVVPLTVLIIWMFFLNRRPEEAGLLAVASALLVGLFTPGIS
                     LGPRELLNILINTGRGMLEIAAITGLAGVVIGVLQLTGLGFTLTLTLLNVGQNSAILL
                     LILTAIVSIILGMGMPTTAVYVLLAVLVAPGLSKLGILPIAAHLFIFYFGMLSMITPP
                     VCLASYAAASIGKTDPVKTGWEAMRLCAIAYVVPFLFVFSPALLLIGSWYAVVISIAT
                     AIVGAVLLGVGLVGYLFRPIGVLRRLLFLLAATGLLIPVAETGTYTTLTWAINAAGVV
                     LAVFMIAIEWLARSRLQESKIAAAAKASAGG"
     gene            complement(22380..22982)
                     /locus_tag="VF182_10235"
     CDS             complement(22380..22982)
                     /locus_tag="VF182_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437498.1"
                     /translation="MKKSLLRNLSAVFLLIVFASSGFAVAVSQQDGDRLQAKIDAIVK
                     NAAANPPKSGEFSIPEREANSYLVFNLKEKFPKGLANPEITMMDDGALAARVLVDMDE
                     VRRRRQSPGLFDPLNYLSGQVPVNARGILRTREGRGQLYLRSADIGGVPLPKPLLQEL
                     VGFFSRTAQNPKGFDIDAPFDLPSKIREISVRAGESVVVQ"
     gene            23137..24810
                     /gene="ilvD"
                     /locus_tag="VF182_10240"
     CDS             23137..24810
                     /gene="ilvD"
                     /locus_tag="VF182_10240"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX6437499.1"
                     /translation="MAENLKPRSHVITDGPDRAPTRAMLKAVGFTDDDLSRPIIGVAN
                     TWIEIGPCNFHLRRLAAKVKEGIRAAGATPMEFNTVSISDGISMGAEGMKCSLISREV
                     IADSIELVARGNHFDALVCLSGCDKTNPGVVMALARLDIPGLALYGGSIAPGHLGDRD
                     LTIQDVFEAVGAYSSGRIKEEQFKSVENSACPGAGACGGQFTANTMSTVMEFLGISPM
                     GSNGIPATAAEKDEAAFNAGKLVVELLRRDLRPSQILTRKSIENAIASVAATGGSTNA
                     VLHLLAIAREAGIELNIDDFDRISARTPLIADLKPGGRFVANDLYKAGGIKLVAKRLI
                     EAGLIHEDTLTVTGKSLREAAQDVQEETGQEVVRPMVNPLKPTGGLVILKGNLAPEGC
                     VVKVAGHERLIHKGPARVFDREEDAFAAVQNGGIRADDVVVIRYEGPKGGPGMREMLG
                     VTAALVGAGLGDSVALLTDGRFSGATHGLMAGHVAPEAAQGGPIAALRDGDTIVFDIA
                     ARRLDVQISQEEMNARLRQWAPPAPRYTSGVMAKYAKLVSSASQGAVTL"
     gene            24839..25138
                     /locus_tag="VF182_10245"
     CDS             24839..25138
                     /locus_tag="VF182_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437500.1"
                     /translation="MAEWTLWAALFTLGTALAMVALYLICRERFQAMSTRIAALEREL
                     EAARLREEANEKNLSKILDAVMELRGSTESHLENLRHGLDQLRAENYSHPSVDAA"
     gene            complement(25266..25844)
                     /locus_tag="VF182_10250"
     CDS             complement(25266..25844)
                     /locus_tag="VF182_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6437501.1"
                     /translation="MRMKLLAVAAALVVLGGASPALLKSAEKTKQEKIVKDGSVVSLQ
                     YSLSEEDGKLIETNKGKDPLKYTQGNRQIIPGLEKELAGMKVGGEKRVKVKPEDAYGV
                     VNAKAFQEVPKEQIPANGLKLGATLSAKGPQGQEIPVRVHEIKEKTVVLDLNHPMAGK
                     TLLFDVKILDIQPSAASSSAPPPKPAQTPQPK"
     gene            26226..27659
                     /locus_tag="VF182_10255"
     CDS             26226..27659
                     /locus_tag="VF182_10255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6437502.1"
                     /translation="MTDLPAKLQRLALINVCLGQFMTALDSRSIIVALPTISIYFNSS
                     MAVVQWIPLAYQLTIIGFVLSLARLGDMRGRKKIYGSGFLLLALGSACSGLSTGLWQI
                     IAFRVLAGVGGAMVLANGRAILSIVYAQHERGRALGFASMAFHLGYISGPSVAGVLID
                     TVGWRWIFFLNLPVALTAAYMARKVLPETPIQRQTHALDLPGMLTLFATAVTLILGLQ
                     QIAKSGITWVPLAIFLLSVVAGALLFHFERKSSAPLLDLSLFKIRVLTAGVLSHLFVV
                     ISHSSTFFLLPFYLQGILHFSPTQVGVTVIFFSLAIVFLAPLGGWLGDRLGSRLLCTT
                     GSALSLISMLGFSRLGADSGYIAVMIPLMILGVGWSIFQAPNLSAIFSAVEPRYVGAV
                     SGISLTSANIANAVGVAIGSALFLRWLNRYGLDQSGVPPYTQWAENPAVFIRAFQNSW
                     LLIAGLTVIAIVASSMRGVDKRQGQGS"
     gene            complement(27732..28328)
                     /locus_tag="VF182_10260"
     CDS             complement(27732..28328)
                     /locus_tag="VF182_10260"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6437503.1"
                     /translation="MAKLVDPWDHHDWSSQQYVNQWAEGQDEREVERQEIFRLIATTL
                     SDDSQAPLNILDLGAGYGALAQFLLKHFPNGRALCQDGSEEMAKLGRQRMEHLTGRFD
                     YVLCDFSKPGWRRKIEGPFDAVVSAIAIHNVRSPKIIKSIYAETFLLIKTGGLFLNFD
                     RMTPSKEDQLAWLQEAGFRNVKCLWDGGRRALVGGYRT"
     gene            complement(28413..29240)
                     /locus_tag="VF182_10265"
     CDS             complement(28413..29240)
                     /locus_tag="VF182_10265"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6437504.1"
                     /translation="MSNVKLPSGVDLYYESHGQGEPLILVPSTAYSGEVWKPSQMPLT
                     NSLNLILFDPRGCGRSIPIQEVYTIEQMAVDIVALMDHLKIASAHLLGHSMGGRIALS
                     LTQNFPGRVKSLIMAASGSGPAARLGTDCIPGLPHRLVLEMIELGFEKFIYNEICESD
                     TFFTEVYRRQNRDKVEEFYRLAWATHAKLPEFIHLCMARHNFEGTHRLGDVKAPTLVL
                     IGDADKSGSNHVAQSQILADRIPGAVMKILHGQSHGFFWQAPEETNTAILDWVRRHS"
     gene            complement(29352..>29559)
                     /locus_tag="VF182_10270"
     CDS             complement(29352..>29559)
                     /locus_tag="VF182_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437505.1"
                     /translation="DMLEATYGEARSYIETVPYATKKGLEAIISELASAETKSKAAKP
                     DDFLDTRFVSQLEKEGFYKPAAAR"
BASE COUNT         6365 a         8062 c         8390 g         6742 t
ORIGIN      
        1 cgtgctgatc cgggcggata aggaccagcc ggccctggta aagctcacga agctcgggct
       61 cggagcgtcg caagacggtg agcggcactc cacggttgca cgcggcacgt tcgaggctgg
      121 agcagtcgat ctcgctgtgt ccgagcttta gaagcgtgaa gccttgacca aagtggtcga
      181 acaacgccgt gccatcgggt agccaggtat gaggggcgcg gtgacccggc tgagctgttg
      241 ggatgtagcg cgaggtctcg tacggcgtcg gtggcgtgcc gtccggcaaa ataaccgggg
      301 aggtcgagta gcgatagccg aggcttacac ccaaactctg gaagttcttg ggcttggtca
      361 gctgaatccg ccccgccaac gtttggcgta cagttgcacc atggacgggg tcttcgagcc
      421 tcggcgtaag gagttcgttg tcttctgagt tctggatcga ttcttccaag gctctgatat
      481 ggaattgacg gcgctcgatt tcgtacgtct ccagcaagtc ctcgccgccc cagccttgga
      541 ggaccgcggc caacttccag ccgaagttga cggcatcgcc gacgccgccg ttcatcccat
      601 agccgccgct cggcgactgc agatgagccg catcgcccaa gagaaagaca cggccttgat
      661 gataacgctc cgctaaggcg ttgtggacag tccatggtgc gacgtgctgt aactcaaaag
      721 ggatcggctg gccaacgatg ttgcggatta cctctgctgg atccagtctg tcaggatcga
      781 cgcccattaa gtcgaagaac catgcgtcgg gcgggtgcaa cggacctacc accgcgaggt
      841 gcggcaaatc gtcattcaag acgttgtaat ggaaggcttt ctcaaaggcc agcgtggacc
      901 atagcgttgg cgcgcggaaa acgcctccga gcgcgtgttt gaggcctggc caatgatcca
      961 ggcggatgcc taacagcttg cggactgtgc tccggcctcc gtcgcaaccg gccacgtact
     1021 cggcggtaat ttcctggcgc gctccgctct ccacgtgcac gatctccgct cgcacctcgc
     1081 cgcgccgctg ctcgaggccc tcgagcttcc agccgagccg gagaatgaca ccgggcatct
     1141 cgcgtacttc ctcgcgcagt acgggttcta aaaacgattg cgggatgcgc aatgttcgtt
     1201 cgggactgaa gcggttgccg ctatcggccg gggtagcatt gtgcaacgcg gtgatcagct
     1261 ggccggttaa ccgcgtgacg tacaagatcg tcgatggaaa gtccgcgcca agaggcgccg
     1321 ccgcacgcaa tttatcgggg atgcctcgcc cccagcgacg aaacagctcc aaggtacgct
     1381 gatgcaccgt cttggctttg ggattgccgc tgaagtgctc attcggttcg actagcaaac
     1441 agcgtatgcc gcgccagccg agctccagcg caaggcttaa tccaactggt ccggcgccga
     1501 ctatgaggac cggtattctg gtgtgactgt caggagacat ttcagttgtg gtcatgccta
     1561 tggtttaggg cgcgagcctt tccttttcga ggaccgttca attactccaa ttcatcgttc
     1621 actacggtat cctgccgggt ggtattactt ctccggttca tactgcaagt tgtcacggat
     1681 caggttggag atgccgcggg cgatgggccg gcggatttct tcgccgacat gagcgagccc
     1741 gcctagggcg cggcccaaga tcgcaaagct cttggacatc tgccaatgca accccatgtc
     1801 catcgagatt gccgcgatgg cgccagtgac gtttaccggt aaatgtctgc ccacccgttg
     1861 gtctgcgact ttgccgatct ttcgcagcag ctcgcagtaa cggccgtaga cttttgtttc
     1921 ttgcgcaact tgaaataatc tctcggcgcg cggatcaccg ccggtgtgaa taccatggcc
     1981 gatgcccggc acgacttgtt tattggcgag ccgcttttcc acggctgctt gagcgatggc
     2041 gtcgagatcc gtttctttcg agtcgggcgg cagcgcatcg tgaagcattt tcgcgcagtg
     2101 ttccgaagaa ccgagatgaa cgctgccggc gccgagaatc gccgcggcaa ccgctccctg
     2161 gattgcttca ggcgccgagt gaaacgtcat ccgcgccgcc gccgcgccgg tggtcatgcc
     2221 gtgatcgacc agcacgatca gcatggtatc gatcatgcgg ccttcttccg ctgtcggcat
     2281 gcggccggcg atcattaaat aaaccatttg ggcaaaagag attttgccga gcagttgttc
     2341 attcaaatcg tagccgcgca cgaaaatttt atcgagctcg gctttgccca tcgaggaaac
     2401 caatttttct gtttcagcca ttaatcctcc agtttatgtt tcaggtttcg agttatccgg
     2461 gaatatagaa tcaagttaac gaacaatcta tgaagatgtc gaggctcacg aatccagacg
     2521 caacagccgg gcggcgttct cgcccatgat ctttcgtttg tcatcagcat gaatatccgg
     2581 acgatcgatg actgcctgtg gcgagccgga cagcttggca tcccaatgcg gataatcgga
     2641 cgcgaacaga acccgctctg cgccgatgga ctcgatgacc tcacctaaac gggtttcatc
     2701 gggttcgcac gaggtgaagc actggcggcg gaaatagtcg ctcggtttca ttttcagcca
     2761 tggcacttga aacggtagaa tctcgtaatg ctcatccatg cgccacagcc agtagggcag
     2821 ccagccggcg ccggcctcga gaaagacgac ctgcagcttg ggatggcgtt ccagagttcc
     2881 cttgcagatc aaacccaagc tggcaagcat ctgctcgaag ggatgggaaa tcgtatgcgt
     2941 gaaaaaccac tgaccggcga aacggtcgac accggcggtg ggcagataag agccggaacc
     3001 ctcgtggacc atcaatggca cacccaattc ttgcagcata tcgtacagcg gatcgtagta
     3061 gggatcatcg agtgtgcggc ctttgaccgg attgggccgg gcgaaaattg ccttgaagcc
     3121 gagttcgtta actacacgtc gggtttcggc aactgcggcg ttgacgtcct gcacgggaat
     3181 cgcgccgact gccattagtc gcccgcgatc ggcgccgcaa taatcgtaga gccagttgtt
     3241 gtaggcgcga cacaacgccg ttgcgtaagc ttcctcttcc accgacgtca ggtacaagcc
     3301 ggccgatgga aacaacagac cccgttcgat gccgtcgcga tccatgtctt tgagcctggc
     3361 gcgcggatct atcgccagat cgcgatgctc gttgaccggt gagccgtcat tcttttcacg
     3421 gaaaggtgtt tcatcaggct tgcgatggtg gcgcttgcct tccagaagaa aaagtagctg
     3481 gccattggcg actctctcga tctttggcgc ccgggcgcga aacggctgtt caagataacg
     3541 cgcccataga tcgtcggcct ctcgaacatg cccgtcgata tcgatgacac gcattgcttg
     3601 ctccgacatt cttatctccg ttcgcgcaga acctcttcga cgatgctgga atccaccacc
     3661 cgatccaacg gcaggtcagg ttttccttgc tgctcgagaa cggttttgat tgcttccgcc
     3721 aagggtttgg cgtcgtcgac gataagccgg gagtagatgg catgagattc ggtagcgaca
     3781 tccggctcca gatcaaaata cttctgaaga aatggcacga cttcggcttt ctggtttttg
     3841 atccagtgca gagtatccag ttgcgcgcgc accatcttcc tgacctgttc gcgttcctgc
     3901 tggatcttgc gcgtcgacgt gccgatgccc accgtcgcca tatcgatgac atcgccggcg
     3961 ctgcccaaga gattgtagcc ttgccgtctg gcaatcacga agaaaggcag tgaaagtgcg
     4021 gtggcatcga ttctgccggt tgcgagcgcc gggaaacggg tgtccgatcc gcccatgttg
     4081 acgaagctga catcgcgcac atcaagcccg ccgcggcgca ccaaaagacg cgcaacgaaa
     4141 tcatcggcag caccgatggt gacggcgatg gttttgcctt tcatgtcggc catcgatttg
     4201 atgctcgacc gcgccaccag ggtgtgaaat gaagtcctaa gcgacaggct gacgatcttt
     4261 accggtgcgc ccgacatgct gccacgggag atcgagctgg cgccgaaggc ataatcgatt
     4321 tcgccggctt gaatggcgcc gatggtaagc ggcgggcgca tggcgatgat ctctgcttcg
     4381 atgccgtggt tgcgatagaa gccgccctcg cgcgctgccc acacatccaa aaatcccagc
     4441 gacttcgacg atactgcgat gcgaactttc tcgaggcttt tctcggcaca ccaggcggga
     4501 acaacagcga caatgaccag aatcaaactt gcccaagtca gtgcggccga tgttcgcttc
     4561 atgcattcac ccagataaga gtagcgcgca tgtttggctg catactacag gaagtaatga
     4621 cggtcaatcg aaggtgacgc gaaattaaca attaaatcgt tcaacatttc aacgtaaaag
     4681 ccgtgagggg ttatcgcagg agtagccgcg aagaactgtt gcaagcggcg agtggttcaa
     4741 gatagattcg cgccaagagt tgcagcgcca gggatgaagc atcgagtgga agcgaacagt
     4801 ccggaggtta gccatgccta actatgcagc taagttgacg ttcggtcccg aggcagcgga
     4861 ttggcaggag cgtttcaatc cggatcgtat gcgccggcag cgggttgaac gcgcgcagat
     4921 aatgatgcgc aaatacggcg tcgccgcgct gctcgaagcc aatcaccaca acattcgcta
     4981 tctcaccgcg ctcaagggtt ttgcctatcc catgtgccgc tacgtgctgt tttttgccga
     5041 gcacgatccg gtgatgtacg agcataacgg ttactatcat cagatgccgg accaatgccc
     5101 ctggatcaaa gagtggcgcc cggctcgctc ctggctcacc ggttcgccgg gtcccgaagc
     5161 ctgcgccgcg gaggccaaag cctttgccgc ggaaatcaaa accgagctcg agaaacgcgg
     5221 gctccttggc gagaaactgg gtttgggcag cttcgacggc aatgcacgcg aagcgttggt
     5281 caatgccggc gttaaaaaca tcgtcgatac acggcagctc ctgctcgaag cgcgcatggt
     5341 taaaaatcag gatgagatca gctgcttgaa gacctgcgcg gcgatggttg atggcgtctg
     5401 gtatcgaatc tgggagatct tgaagccggg gatgcgcgat acggatctca tgacgatcgg
     5461 tgctagcgcc ggctatcaga tcggtttgga agccgccgtg ccgggaggct ggcgcagcgg
     5521 gccgatgact ttcgatcgcg gctgtcacgc aaccaaccgt ataattcagg taggcgatct
     5581 agtgtacggc tcgtactgcg gcgccactta catgggctat ggcagttgca cgtatcggac
     5641 attcatggtt ggccgcaatc cgacgacgaa agaaaaagac tggtataaac aggtgcgcga
     5701 gcgcctggat gcggtgatcg aagagatcaa gccgggcaag acgacagcgg atgcggcgaa
     5761 gcattttccg cccgcgagca aatggggcta taagagcgaa gtcgaggtgc tcgccagtga
     5821 aatcggccac ggcatcggcc tcggcaccag caccggttat gacgctccga tcattaaccg
     5881 gttgtggtca ttcgagcatc cgcaacttct cgaagagggc atgaccatcg cggtggaatc
     5941 ccgcgaggga gagacccgcg tcggcggcgc gcgcctggaa aacatgttag tggtaacaaa
     6001 aaacggcgcg gagatcatgg actacttccc gcgtgacgaa atactcgtgg cgccgcgatg
     6061 atcagctggc gtgaccggga gccttaatca taggaccaag gaggatttat gtctcagcta
     6121 cgttgtctca caatcaaagc gcaagagccc agtcgcgttg cggattttta ccgctcggtt
     6181 ttcgaactga aaaccgtcac ggaagatccg gagttggttc gtctttcgga cggcgtgttc
     6241 acccttgccg tcctcaaaga gagtaaggag gattccaccg gactccacgc cagcggcttt
     6301 caagtcgaag atttgggcaa ggttgcgcag aagtcagaaa agggcgggca ggcgttgccg
     6361 caaagcgagg ctcaggtggc cgaccccgat cagaatctga tcgatcttac gaccaatgac
     6421 ttcggtcttg cgccggagaa aacccgctat cccatccggc atatcgcgct ttacaccgcc
     6481 gatccgaaac gactgtcgaa tttttacggc gacgcgtttg gcatgaagga agtcgctcac
     6541 agcgatcgct cgtcgatctt cgtttcggat ggctacttca atctcgcgct cttgtaccag
     6601 cgagtgcgtg aggaaaaaaa tggctttaat catttcggtt tccgcgtaga tgacctcgag
     6661 cagatccgcg atcgcgccga aaaggcaggg acacgccgcg gcgccgcccg cccaagcaga
     6721 attccgtttg ccgaagtccg gcttcacgat ccagaaggca acggcgttga catttccgtc
     6781 aagggatggg acgtgtagtc gagcaaccgc aaacagatcc gcggttttct ttagcgaggg
     6841 gtatcgatct gcgcaaaatg gctgatcccg ccctatcgcc tccgaacctc gacacgtgga
     6901 agaaagtttc atgccgatcc aagccctgca acacgttacg gtgattgagt gcgcgacctt
     6961 tgttaccggt ccgtacgctt cggccctgtt ggccaacctc ggtgctcggg tgattaaaat
     7021 tgaatcgccg ccccgagggg atccgtaccg ttattttgct cccgatccgc attacagttt
     7081 caatttcgcg catctaaatc ggaacaaaca aagcctggtg ctcgatctca aaactgcgcg
     7141 tggaagaaaa atttgttccg agctgcttaa aagcgcggac gttttcgtcg aaaattttcg
     7201 tcctggaacc gtcgaacgtt tgggcctggg gtacgaaact ctgcgcgctc tcaatccgcg
     7261 gttagtttac tgttcaattt ccgccttcgg tcaaagcggg ccgtatgtcg ataagccggg
     7321 attcgatacc ttggggcagg ctgttagcgg attgctgagc ttactcaacg attcagacga
     7381 tccaaaggtg atgggcatgg ccctttcgga ctatattacc ggaatgtcag ccggctacgg
     7441 cattctcggc gcgttgctgg cgcgcgagca gtccggcgaa ggttgccgcg tggaaacctc
     7501 gctgctgcag gcgacacttt ctttcatcgg cgaaagcgcc gcgggctatc tgcgcaccgg
     7561 gagtgtgccg aatcggatgg cgcgggttaa aaatgcccac gcgttcgcgt ttatatgcgg
     7621 ggacgggttg ccgctggcga tccattgttc tgtgccggag aaattttggc tcgcgttgtt
     7681 agaagcgacg ggaaggatgg acattgccgt cgaccaaagg tttgctacgc gagatgcgcg
     7741 ccggcaaaac tatgcatgtc tcgaagaaac cctggcatca acatttaaaa ccaaaagcag
     7801 gaaagaatgg ttgcgctgcc tcgaagcctg cgatgttccg gtcgtgccgc tgcacgacat
     7861 cgcggaggct ttagccgacc cgcagattcg tcatctccga cttatcgaaa ctgtggagca
     7921 tccaattgcc ggcccgatgg agtttgtcgg cgcgccggtg cgataccgca atttcaccga
     7981 atctccttcg ctgccgccgc cactcctggg cgagcatacg aatgctattt tgcgcgagct
     8041 cggttattcg gaccaggcga tcgaagaggt acgggccgag caagtgatag gaattgcgga
     8101 tcaggcgccc tgacgtgatc catgattaac gaataaaaca gatcgaagaa gaaagttatt
     8161 gcggctttca ttgtggacaa cactttgttc tggcttttgc ggccatcctc gtatcgctga
     8221 cggtcggtac cgcggccggt gtggatcgag tttatctttc cggcttgcag gcactctggc
     8281 ttaccgccat catcggttcc gattcgacag tactcaccat ttgaaagaag aactgtatcc
     8341 tcccgcgcga aagacgagat cgtcacaacc ggaccctgga ttgttccccg ctttaacacg
     8401 actaacgcac gagccctgca ggtgcaggcg agccagtgat gcgtggttca actcgttcaa
     8461 atactttcaa atcgttccca gcctccacct gtttcctccc ccgcgaaccg cgggggagga
     8521 ttaaggaggg ggttgaacgg cttaaacatc ttgaacattt ggaaccccgt tcggcggcag
     8581 tccgcccctc acattgacgc agtctcgtcc ttcttcttga gtcatctgtc atccggacta
     8641 gtcacgggcg tatgatttga cttgagcgaa tgcgtctgac tactgtacct cgtatttcga
     8701 ggctgaaggg caaaccttca aggggtgcaa aaagaaatga tttacgatgt tgaaataaat
     8761 tcggcggcgc actatccggc ggctgaagtc cctggcttgg tcgtgttggc tgagcaggtc
     8821 ggcttcggcg ctttttggaa aggcgaatcg aacagcaccg atcccgtggt tatgctttcg
     8881 gctgtggcga gccgcaccaa gaccatcaaa ttaggtacgg caatttacca tatctacggc
     8941 cgcagtccgg ttacgttagg aatccaggcg gcaactctac aagacttatc gggaggtaga
     9001 ctgctgctgg gattaggcgt tgcgaataag agcattgccg gatggcatgg cggagtcttc
     9061 gaccggccgc tcaagcgcgc ccgcgaatac atcgatatcg tccgcaaagt tgccgccggc
     9121 gagcgagtcg aatatgaagg cgagatttat cagaccggca aacgttttca gctttcgtgg
     9181 aagcctgccc atcagaatgt gccggtttac ttggccggac tcgggccgca gatgaccaaa
     9241 ctggttggca aaatatccga cggtgttttc atcaacatgg caactccggc gaaagtcaaa
     9301 gagattgccg cgcgggtgcg cgagggagca gtagaagcgg gccgtgatcc aagcaagatc
     9361 gagatcatcg ccaagtcgcg ggtttcactc aatcccgaca aacaggcggc gcgtaacaag
     9421 ctgcgccagg tactgacgtt ttacaatatc gccgatcact acagtgacat gttgcgcggc
     9481 atgggattcg aaaaagaggt gaacgcgatc cacgaagctt tccagaaagg cggcttcaaa
     9541 gcggcgatgg gcgcgttgag cgacgactat atggataagc tgccggtggt cccggccagc
     9601 gacgtgaaag agatcaagga aaagatgaag gagttcgagg aagccggagt gacgcgcatg
     9661 gtaatcccct acgtgccggt aaccgagccg gtggaagacg caaggcgatt cctggaggcg
     9721 tggggaaagg catgaaagac gtgatcgtga tcgcgggaac gtgatggatc ggatgccgaa
     9781 aacgaacacg tgccatcaac acgaacgccg aagaacaact cagtaccaca atctgaaaca
     9841 gtcgttagaa ggaggagatg atgtgtgatt ttgctgcaga cgccgcggcc gcggaacaac
     9901 tgatggtcgg tcgcactcta agtgtggagc gggtgcgcca gctcaatgcc aaggattatt
     9961 tttatcaaat gctcaaggac aatccggaga tgctgaagat ttatccgggc atcgagaacg
    10021 aattgcttga gggggctata gactgtcaca tccatgcttt tccggatttt gtgcaccgct
    10081 cacaggatat gattcagatc gcgatcgagg cttcaaaatg cggcatgcgt gccattgctt
    10141 tcaaagacca ctggaacata agcgcgacgt cggcttatct gacgcagcgc tacatcgacg
    10201 acatgatcgc ccgcggcgag cttagccatc gagttgaagt ctacggcggg gtcggtatgt
    10261 gcttcggcat gcggcccgaa tatgtccggg tgggactgca gtatcctaat ttcaaaatga
    10321 tctggtttcc gaccttcacg tcctatggct tctggcgcgg cgccggccat ccggaaaagg
    10381 aaggggtgcg cctggttgct gaagacggca aggtcctgcc cgaagtgaaa gagatcatgg
    10441 agatggcggc ggaaaagaga gtgggcatcg gcttcggcca caccgacttc caagaattgc
    10501 tgccgttggc gagacttgca aaagatatcg gtgtccgagc gaccctggat catccgttgt
    10561 tagagttgaa caagctgcta ttggatgaga tgaaacaact gcgcgatctc ggcgtctatg
    10621 tcggcactta ctgccagccg atgattccga gtctctatca gcctgtggcc gatccgatgg
    10681 agaccatccg cacgatcaaa gaaatcggtc ccgaccgctg catcatcggc agcgacttcg
    10741 gccaggtgtt gcacatggat gcggtcgacg gtatgcgcgt cttcattcgc gctttgctcg
    10801 gattcggcat taagccggat gaagtgaagg tgatgttgaa ggagaatccg gcgaaattga
    10861 tgtggctgga cgagccccgg tgaaaaaggc ccaccgcggc gttacgcttg gccggctcgc
    10921 tccaacgtac tacaacgtac gcgtccgcgt cgatcttagc gtgcctcgca tccgggacct
    10981 attgacccgc gcctcaaacg tcaatgtttg aggaattaaa atacgtcgtc gttttaggtg
    11041 gctgcgtcgc gagacacagc cgcctaaatt ctttttggta taagttcacc accatctcct
    11101 aatatcctgg tgattaaagg caatggcatg atcttatcta actgccagcc actgagggca
    11161 atgagggggt gccgactgtg cacagcttcg atcgcgttta acgttgcatg caaggcggct
    11221 atcccagtgg tcgcgatagt atcgcaatag cgagtgtaaa tcccgtgcct gatacgaatt
    11281 gatacaccta gtcccatatg tttctatggt ccaattctaa aagggtcaga aagggacctg
    11341 ctttcagtta aagttgccca gttaactttt tggctagaca gttcgacgat ggcatctatc
    11401 ttgcgctttg acgacggatg agcttagcag aggcaacttg cgagcattgt gagggtatcc
    11461 tcctcggcaa caaagcgtat cgggtgtgga ctgaagattc cgggctaatc ctgttagatg
    11521 tggtcgtatg ctacgcctgt catctggagg cgcaaaagct tggccttaat agcgacgaaa
    11581 ttaagcaaat cgccagacca tcacacacgt aacctgtgac gaggtggcgc ctcaatctcc
    11641 cgccgaagca gtcgtggttc ccacaattca cgtttaaggc ccgttgcggt aatcactggc
    11701 ggcggaacaa ttctgatgca caacgattac gcgtatgatc aacgatgaac cagtgattgc
    11761 tgctgagaat acgatagccg gtcacgagat tcgtccaacc aagcgcggat ccagttaaca
    11821 tctttatagc tggcgagtct catacccgaa cttccagttt cgctagcgtt accccgcaga
    11881 gtttagccgg cgcgccggcg ctggaagctt actactcgat aaagatatcc gaggatgatt
    11941 aggccgatga ccacattcga aataggattc atccaatgag ccaccttttc gtactggctc
    12001 tcgaggaagt acccggccgt acccaggagc gcggtccata agattgtgcc ggcggcagaa
    12061 tagagaagaa aggagccgag cggcatcccg gcaatgcccg cgggaacgga gatcagggtt
    12121 cgaacggcgg gaataagccg cccgatgaac accgctttgc ctccgtgacg attaaaccag
    12181 gcgcacgcct gctcgacttc gttgggcgaa agcgtgagcc agcggccatg ttttgccgcc
    12241 caccgctcta acctttcggc gcctattttt tgaccgacgt agtaccagag aactgctccg
    12301 agcagcgaac cgatggatcc cgcgatgatg acgatcagca agttgcgctc gccctgagcc
    12361 gccgaaaacc ccgccagcgg catgatcagt tccgacggga ttggtgggaa aacattttcg
    12421 gcgaacatca aaagagcaac accgactgtt cccatgtttt caaagactgt aatccaatcg
    12481 gacacgactc ctcctttcca tgacttttct gccacggcgc gcttccgtcc atgcttcagg
    12541 tatcatcatc gcgatcactc tcagcctttt tgtagaattc aacgaagaag cgcacgccgc
    12601 cgattggttc gacatggtgc cggatctctg gaatgacgat tccgggccgt tcgggagata
    12661 actcaaactg cacgcccaga gcgtcgattc tatagttcaa ttctccctcg atcacctgga
    12721 tcttggccca cacaccgggc ttggtggagt gatccctgcg cagactccga ggtatggttc
    12781 gttcgttgaa ctcgccggtc tggcggtaga ctttaaaatt gtccggcatt tcgaagcgat
    12841 cacacctgat gcaatctaac ggcttaccga gcttcttgct ccgcccaagt ttacttatca
    12901 cccacggccg ttcttcaaag ggtggccgat gcctgacatg ctgcttatgg ccgcagctca
    12961 gaaaagctac ccagtcgccg tattcgtcga ggttgaatcc ggttatcgag cggcgcactt
    13021 gactaagtct cgcaattcat cagaactgat cggagttacg cgatctgctc gattgcgggc
    13081 tcgaccatag agtgtaaagc gaacaggaga gttgagcttt gctctcgtgg gcctctgttc
    13141 cttggacaaa acggattatt cagttgagct ctacctgcac agatcgccgg tgattattgc
    13201 cgtgtgtatg tttctgttgt acgtgtctcg cccaatttgg aatttatgtt gatggttttg
    13261 ccgccttcgc cgaatcggat ggttgccgac tggcttctgc cggcaacgtt cggcggggcc
    13321 gtgaaagtta acgcgagatt attgccggtg agacgccagg ttcccgaacc tttaacagaa
    13381 ctgccggaga atgtggcggt gaacgtgcca ttcggatcaa attcgatcat atcgttgccg
    13441 ctacacgaag tcgtcgagcc gcccggcaac ggcatagtgg cgggacattt tgccgactga
    13501 ttgccggtgg caatcgatgt ttggcgccat cgcccaacca gcggcgatga ggcagaaggt
    13561 aagctgacac tttctctggt actacagcca aacagggaca tcagcagtaa aaaaccgctc
    13621 atcgaaattc ggccaaagct ctgctccatc gattgtttct ctttcagtcc tgtgtacgtg
    13681 ggcccctgct tcttcttaac ccgacaggtt gctattgcca atggctctat gacccgaatg
    13741 agtgttctct ggacaaggat tgaccggaac tagaaggcag gctaggcata agacaggaat
    13801 caaaggaggg catcacgatg agaacaacct ggttattgat tgcttgtgct ttgatgtcga
    13861 gcgcttcgct gggggaaggc gccgagtact ttatctaccg agatgctgcg gggagaatcg
    13921 tcctggccaa tgaaacacct cctcccggag ccgagatagt taagcgccat gaattgcagg
    13981 atgtcagtga tgaagcggtg cgcgcggccc gggagcacga ggaatttttt tggcgccgcc
    14041 taaaagacga acagcttgcc gagtcaaacc aaaggctggc cgaaagcaaa gatcgtttgg
    14101 ccgaggccat catcacggcc gcagcgctgc gtgaaaccga gccggaggtt ttgatacagg
    14161 tcgcaagttc tcacgattct cgattccgcg gccttcgttt ggatcagggg cgaataccag
    14221 cacgtccacc cgccggcttt cgccatacga agatccgcta agcttgtcgt ttgtcgtgtt
    14281 cgactcttgc cgcgtgacat tcgagcacat gctgaatcat gccgattgcg agttcgtgct
    14341 cgccaagaaa cactttgccc gcctgctctt tttccaggag cacggcctcc tcttcgctgt
    14401 gggtgcgcac gacggtctga atgtgcggat taacagtgcg ggcgatctcg atcatttttc
    14461 gaacgttgag ggtatcggga gtggcgatta ccaaaataga tgctcgatga acatgggcct
    14521 ggaggagaac cgcgggatcg gaggcatcgc ctgacactgc cgctatgccg ctcgagcgca
    14581 ggcgctcgac gatctcccgg ttctcctcgg caacgacaat cgatatgcca tgcgcggtca
    14641 gtgcctcgcc gatgcggcgg ccgacccggc cgtagccgac gatgacaaca tgtccggtca
    14701 ggcgctttag atctgtggtc atcggcaatg ccgcgagcgg atcatccggg cgttccaaag
    14761 tttgagcgag ttttgaacgc gaacggatcc aggcttgcgc cggctcgatc gctccaaaca
    14821 acagagggtt gagagcaatc gaaatgatgg cgccagacag gatcaggctc tggccttcag
    14881 tgggcaataa accaagtgaa accccgagac cgaccaggat gaacgagaac tcgccgattt
    14941 gcgccaggct tgcagagact gtaagcgcgg tattgagcgg gtagcggaag aggagaacga
    15001 taaaaaaagc cgccagcgat ttgccgaaga tgatgatcgc cagcaccatc agaacgtgga
    15061 gcggttcact aatcagcacg tcgggctcaa acagcatgcc gacggacacg aagaacaaaa
    15121 ccgagaatgc gtctctgagt ggcaacgatt cttccgccgc ccgatagctg aggggggatt
    15181 ctctcatcac catgccggcg aagaatgcgc cgagcgcgaa tgacacgcca aacagttcgg
    15241 cggacccata ggcaatgccc accgcggctg cgacgatgca caaagtgaaa agctcgcgag
    15301 agccggtccg ggcaatctgc cacagcaacc agggaaataa gcggcgacct gcgacaagca
    15361 tgagtacgat aaacaccgag actttgccga ccgtcagcgc gaatgctttc agtaagcttc
    15421 gatctcctgc agattcagtg gcaccgccca gccacaccga tatcgctgga agcaggacta
    15481 gcaccaacac catcacgaga tcttcgacga tcagccagcc gatggcaatg cggccattga
    15541 ccgaatcaag aacaccgcga tgttccaagg ctttgagaag cacgaccgtg ctggccaccg
    15601 agagcgccag accgaacacc aatccggcgg caagactcca accccaagta gcggagacag
    15661 ccattcccag cagcgtggcg accgttattt ggacaaccgc gccaggcaac gcgatccgcc
    15721 gcaccgccaa caggtcatcg agtgaaaaat gcagtccgac gccgaacatc aacaacatca
    15781 caccgatttc agcgagctga ctcgatagct ccacgtcggc aacaaagccc ggtgttgccg
    15841 gaccaatgat catgccggcc gtgagatagc cgacgagaac gggcagcctt agtcgggctg
    15901 caataaaacc catcaccagg gcaagcccca ggccggcggc gatcgtgctg attaaggtaa
    15961 cgctatgagg cacctagttc ttctcctaaa ttcgactatg gacgcgactt aggtccagag
    16021 agccgaaact gtcgcgagtt ggtgttgcca gtcagccagc ccttgaagcg caccgggtca
    16081 gaaacatcgg ctgcgactta caatggtgtc ttggtcctgg ccccgtttcc gcatggatgt
    16141 gccggaggat gggcgcagag ctggtaggca cagagcgcga acaactttaa tcgcggattg
    16201 agcatcctga tcagcttgaa cggccgatca gacagcggca gtttgttaaa cgattggtgt
    16261 cgggtacagc gacacagacc aaaagtataa tgaataccct gttcccgcaa cttctccgga
    16321 tacaatgagc aaatatgcag aggcgcttac gttctttaga cgtttagaag gcgcgcgttt
    16381 aggcaggagc atggatgctc cgatcagaga taaactgagg cgggttttcg caagctcatt
    16441 atcatgaact ggcctccttc ccctagcatc ctctacccgc gctaatatcc tgtccgcttc
    16501 actttcgtac cgcggcgatt tcagttatcg tgcggacgtt taacaacttc gctcgacatc
    16561 aacttagttt cggaggtcgg ttgccgtggc aagaaagtgg tggctcgtga tcgttggttg
    16621 catcacccac gcggtgaaca ccggcttgag ttattttggc atgagcgcct actttccttc
    16681 cttcgagcgc gagttcggtt ggagccgcac ggccatctca ggcgcttttt ctctggcgcg
    16741 cattgaatcc ggcctcctcg gaccgatgga aggatatgtc accgaccggg tcggaccgca
    16801 gcgaatgata tttatcgggc tgtttctctg cgccggtgga tttttggcgc tgagtttggt
    16861 cgattcgctg ccgatgctct acgccgtgat tgttctcggc atcgttctcg gttccagcct
    16921 gggctacaac atgccgatca gtgttctgat cgccaaggtt tttcgcgaga gaagaagcct
    16981 tgcctttggc atcttccgta tgggaccggg gatttccggg ccgatggtac cgttggtcgg
    17041 atggatgatc ggcttgtacg gatggagaac cgcagcggtg atctccgggt gtgtcctgct
    17101 ggccgtaggc ttaccgctgg cctgcatcat aagaaaaatt tactcccaag aagaagccgc
    17161 cattttaacc gcggagcatt ccggcgccga tttgaaacat tcgccgcatg catcgaccga
    17221 accgcagttc actctcaagc aggcattacg tcatcgatct ttttggttgc tttctgtcgc
    17281 gatgggcatg cgccatttgg tgaccgaagg agtctcggtg cattttgtta ttctcctggt
    17341 ggaccggggt tggagcaccg aagcgggtag caccttgctc gggatctccg cattggtcgg
    17401 cgcgccggcg cggatcggca tgggttggtt gggagatttg ctcgataagc gccgcttggt
    17461 gatgggatta ttattggccc tgagcatctc ggtattgttg atgggctata ccgcccaggc
    17521 gtttattttt accacgtgta tgattgtcta ttcgctagcc tacggcgggc tcgccgcttt
    17581 acaagaaccg attcgcgccg actatttcgg caccagggca ttcgcgacaa tccaaggcat
    17641 gagccgatcg gtcgtcactg cgggcacttt tctggggccg atcattgccg ggtttttcta
    17701 cgatgtgact aaaagctaca ccatcgcatt cggcgttttc gctgtcgtca gcttggtggc
    17761 aacgttgttc atgtttctcg ccaaaccgcc gcgaggttgt gccgattgat cgtggtttgc
    17821 gagctaggcg aaaggatcaa gtgcgctaca ccgaggcagg aaccaatctt cgcagaccga
    17881 gcttgacaac tccgaagcac tcgagcaata cacattacgg cgctggtaat cgaattttca
    17941 atctgcgaaa tcgcgaaagg cggtaacgtt gcgatgagac agaatcgctt tcctgtgatc
    18001 gacggcgacg gtcacgtcac ggaacggaac aaagagattc tggagtttct cggcggcaat
    18061 taccgcggca ccagcaagga ccagagctgg gccgatacgt tctcgccatt tccgtctctg
    18121 gacggttggg cgcggctgtc gggcatgagc tcgccggggc tgcgcgatta tccagattgc
    18181 gacacctgga tctcgtttct tgacgactgc ggtatacaga cgaccatcgt ctacccgacc
    18241 gccgcccttg ccttcggttt gattcaggat cccgcctggg cggtcgacat tgcccgcgct
    18301 tacaactcct ggctgcacgc cagttatacc aagcgaactt cccggatcct tggcgcggcg
    18361 ctgatcccga ttcacgatgt tcccgccgcg gtcagggagt tgcgccgcgt caaagaagaa
    18421 ctgggcatgc ccgcggcgat gctgccatcg gtcaccaacc tgcgcaaagg ttacggtcac
    18481 gagagtttct ttccgatcta cgaagaggcc tgccgcctcg atatgccctt ggcggttcat
    18541 ggcgcgccta gccgcggact gggatttgat ttcttcgaca gcttcgccga agttcacgcg
    18601 ctggagcatc cgctgccgct gatgatccag ctcacccata tgatttttca gggtgtgttc
    18661 gagctctacc cggatcttcg ggtcgcttat ctcgaggccg gctgcggctg gattccattc
    18721 atgatggacc ggctcgacga agacttcgaa aggcgcggta agaagtgggc gccgcgacta
    18781 aagcgcgccc ccagtgaata tatcaaaggc ggttcggtct tcgtttcatt ggaatccgag
    18841 gagcgcacct tgccctatgt gctagagctg ttcggcgagg atcacatctt tttcgcttcg
    18901 gactatcccc acgaacgcca gaggtcccag tatctcgagg atatccccgg catcgaagcg
    18961 cgcgccgata tctcggattc cgcgaagcaa aaaattctcg ccggaaacgc taaacgattt
    19021 tatcgactgt cggattgaat atttgctgca cgagggagga ccggcgatgc atctaacaac
    19081 catcggtgtg cgaatcctct tggcgagttt ttttctgttc ctgccgcaat ggcaaaccgg
    19141 cgcaagcgcg gctgccaagc ttcagtgcgg tccgaccact agtaccagca acgatctgcc
    19201 ccggacggtt gcgattgctt ccaatctggc gggaaccggc gcccatgctt tggcttcggg
    19261 gttggcggcg gtggccagca ggtcgacgcc gatcgccgcc aaagtccaac cctacaacgg
    19321 cccgaatgca tggatgccgc tgatggaaaa tggcgagatc gagttcggca tcatcaacat
    19381 tctcgactcg cacatggcgg tgaccggcac cggcaattac aagaagccat atccgatcct
    19441 tcgcgtggtc tccggcggtg tgtttccgtt taccggcagc atcatggtgc gtgacaagtc
    19501 cgagatcaaa cagcccagcg atcttaaggg gaagcggatg gcatgggatt tcggcggtca
    19561 tgcgattacc cagacgtggc aaaacgccgc catggaagtt agcggtttga agcccagcga
    19621 tgttgttcag gttcgttttt ccaacctcaa cgacgcgatt cgcgccgtgc ccgagggcag
    19681 ggtcgacgcc acttttgccg ccatcggcat tggtatcaac gaggaagtaa acgccatgga
    19741 gccgatccga ttcctcagcc ttcccaatac cgacgcatcc aacaaaatcc tgtcaaaata
    19801 tggcggatcg atcgtcaagc aagatccgac cgccggcatt cgcggcgaca cctacgtgat
    19861 cggttatccg cttcatctcg ccagctcaac caaggtaaac gacaaaactg ttgcgacggt
    19921 tctcaaagcg tggtgggaca atctgcctga actgcagacg attcatccgc agttcaaaaa
    19981 atggaccaag gatgttcagg caatcaccaa tttcacgatc ccctatcatt cgggcgcggt
    20041 gaagttctat aaggacgccg gggtatggac ggaaaaacat gaccttcgca ccaaggaaat
    20101 ctgcagttga ttgaaactcc agctgacaag accagggagg cgtgagaccg gggggttatt
    20161 ccagtgccct tgtcattccg gagcgataaa gttttgtcag gagacgggcg cttggccggt
    20221 caaacagaga gggcgtgcaa agcagacctg cggtaatcgt ttgtcgagtc cttgcgttaa
    20281 tcggacaatg ccggcggtaa atgtattcac aacgtctacg ttgcttttga gaaacgatta
    20341 cgtctctaac cgacgcggag aattaagtgg agatcgaaga aaatgttcgc tcacgtgagt
    20401 tgagcggact tgccggttgg atcactcagg cgcttctgat cgcgattccg gtcagcggta
    20461 tgttcttttt gctcgatgtg ccgcagcggc ttgattggca ggtctttcat gaacagtaca
    20521 tcgggctctt cctcgcgttg actctcgctg ccatttttct tctgattcca ggttcaaaag
    20581 gacaatcgca acgcattccg tggttcgatt atattggagc ggcggccggc ttgttcgttg
    20641 gcttgtatat cttcgttaag tttccggaaa tctccaactc tctaggcgat atttatacgg
    20701 agcgggtgat cctgggggcg atcaccgtga tcctgcttgc cgaggcgtgc cggcgattgg
    20761 tgggctggcc tctcgtcatc attgcaggcg tttttatttt ttatgcgttc ttcgcttatc
    20821 tgtttccggg cgatttctac ggtcgcggct ggtccatcgg ccggctcgga acctatttat
    20881 atctcgatgc caacgggatt atcggccagc ccctgcagat cggcgccagt atcgtgctgg
    20941 tgttcgtcct gtttggcgaa gcgctttacg ccatcggcgg cgccgccttc ctcagcgatt
    21001 ttgctttagc ggtgatgggt cgctttcgcg gcggccaggc aaagatcgct atcgtttcct
    21061 cgagtttgtt cggcaacatc agcggcagcg cggtcgccaa cgtcgtggtt gacggcgcgt
    21121 tcacgattcc gatgatgaaa aaagccggct acccgtcgcc ggtcgccgca gccatcgagg
    21181 cggtcgcttc caccggcggg caaatcatgc cgcctgtcat gggcgccgcg gcattcctga
    21241 ttgccgaata tctggcgatc ccttatgcgc aggtcgcgct ggcggcgttc gtgccggctt
    21301 tgctctatta catcgcgcta ttcattcagg tggatctgct cgccgcacgt aacggcatct
    21361 acggtctacc gccggcggag ctacccagat tgttgccggt gctgcgacgc tccgccggct
    21421 ttgtcgtgcc gctgacagtt ctgatcatct ggatgttctt cctaaaccgc cgccctgaag
    21481 aggccgggct gttggctgtc gcttcggcgc tcctggtcgg cttgttcact cccggtataa
    21541 gcctcggtcc gcgcgagctg ctgaatattc tgatcaatac cggccgaggc atgctcgaga
    21601 ttgccgccat caccggtctc gcaggcgtcg tcatcggggt gctccagctc acgggtctcg
    21661 gatttacttt gacactgacc ttactgaacg tcgggcagaa cagcgccatc ttgctcctta
    21721 tcttgaccgc aatcgtcagt atcatcttgg ggatgggcat gccgaccaca gcggtttatg
    21781 tgctgctggc ggtgctggtc gctccggggc tttccaaact gggcattttg cccatcgctg
    21841 ctcatctgtt tattttttat ttcggcatgc tgagcatgat cacaccgccg gtttgtctgg
    21901 cgagctatgc cgcggcatcg attggcaaaa ccgatccggt taaaaccgga tgggaagcga
    21961 tgcgtctgtg cgcgatcgcc tatgtggtgc cgttcttgtt tgtcttctcg ccggcgctgc
    22021 tgttgatcgg ctcgtggtat gcggttgtga tttcgatcgc cacggcgatc gtcggcgcag
    22081 ttctcttggg agtcggcttg gtaggttatt tatttcgccc catcggcgtt cttaggcggc
    22141 tgcttttcct ccttgcggcg accggtttgc tcattccagt ggccgagacc ggaacttaca
    22201 caaccctcac ttgggcgatc aatgctgcgg gtgtggtgct cgccgtgttt atgatcgcga
    22261 tcgaatggct ggcgcgctcg cgcctgcagg agtcaaaaat tgccgcggca gcgaaggctt
    22321 ccgcaggtgg ctgaatagtc tgtggaagag agcattactc tcgcttctac tgcataccgc
    22381 tactgaacca ccacactctc ccccgcccga acagaaattt cacggatctt tgacggaaga
    22441 tcgaacggcg cgtcgatgtc gaagcccttg ggattttgcg cggtacgcga gaagaacccc
    22501 acgagttcct gtaggagcgg cttaggcaag ggcaccccgc cgatatcagc cgagcgcaga
    22561 tagagttgtc ctctcccttc gcgggtccgc aagataccgc gtgcattgac cgggacctgt
    22621 cccgataagt agttcaacgg atcgaacagg ccgggcgatt gtctgcgccg cctgacttca
    22681 tccatgtcga ccagcacccg cgcagcaagc gccccgtcgt ccatcatcgt gatctccgga
    22741 ttggctagcc ctttggggaa tttttccttt aaattgaaaa ccaggtacga gttcgcttct
    22801 cgctcgggga tcgagaattc ccccgatttt ggcggattgg ctgcggcatt tttcactatc
    22861 gcgtcgattt tggcttgaag ccggtcgccg tcttgttgtg atacggccac ggcaaatccg
    22921 ctcgaggcaa atacgatcaa caaaaaaaca gccgatagat tgcgaagcaa agattttttc
    22981 atctccaaca tcgtaccaag ttccgaattt tctgggaagc cggcaaaaga ctttgcacac
    23041 agatgcgatt tcgcgtgaaa ttaccggtca aggcgagcct tgcaaattag accgggccat
    23101 agttaatttg aacacttatt acagtaggag aaatcgatgg cagagaattt aaagcctcgt
    23161 agtcatgtga tcaccgatgg accggatcgc gcgccgacgc gcgcgatgct caaagccgtc
    23221 ggctttaccg atgatgatct gtcgcgaccg attatcggcg tcgccaacac ctggatcgag
    23281 atcggcccgt gtaattttca tttgcgccgg ctggccgcca aagtgaagga gggaattcga
    23341 gccgccgggg ctacgccgat ggagttcaac acggtctcca tctccgacgg catcagcatg
    23401 ggtgcggaag gcatgaaatg ctcgttgatc agccgcgaag tcatcgccga ttccatagag
    23461 ctggtcgcgc ggggtaacca tttcgatgct ttggtgtgtc tatcaggctg tgacaaaacc
    23521 aacccggggg tagtcatggc gctggcacgg ctcgatattc cgggtctcgc tctttacggc
    23581 ggctcgattg ccccgggcca tctcggcgat cgcgatttga ccatccaaga tgtcttcgag
    23641 gcagtgggcg catacagcag cggccgcatc aaagaagagc aattcaaatc agtcgaaaac
    23701 agcgcctgtc ccggcgccgg agcgtgtggc ggccagttta ccgccaacac catgtcgaca
    23761 gtgatggagt ttctcgggat ttctccgatg ggcagcaacg gcattccagc taccgccgct
    23821 gagaaagacg aggcggcatt caacgccggt aagctggttg tcgaactttt gcggcgcgac
    23881 ctgcggccgt cgcagattct cacccgcaaa tcgattgaga atgcgatcgc atcggttgcc
    23941 gccaccggcg gctcgaccaa cgccgtgctg catcttctcg cgattgcgcg cgaagccggt
    24001 atcgaactga acatcgacga tttcgaccgt atcagcgcgc gcacgccgtt gatcgccgat
    24061 ctaaaaccgg gcggtcgttt cgtcgccaac gatctttaca aagcgggtgg catcaaactg
    24121 gtcgccaaac ggctgatcga agcggggctg atccatgagg acacgctcac ggtcacgggc
    24181 aagagcctca gagaggcggc gcaagacgtg caagaagaga ccggacaaga agtcgttcga
    24241 ccgatggtta atccgctcaa gcccaccggt ggattggtaa tcttaaaggg taatttagcg
    24301 ccggaaggtt gcgtggtgaa agtcgcgggt cacgagagac taattcataa aggcccggct
    24361 cgagtgttcg atcgcgagga ggacgcgttc gcggcggtgc aaaacggagg gatcagagcc
    24421 gatgatgtgg ttgtcattcg ttacgaagga cccaagggcg ggcctgggat gcgcgagatg
    24481 cttggcgtta cggcagcgct ggtgggcgcc ggcttaggcg attcggttgc actcctcacg
    24541 gatgggcgtt tctccggggc aactcatggg ctgatggccg ggcacgttgc ccccgaagct
    24601 gcccaaggcg gtccgatcgc cgcgctgcgc gacggcgaca cgatcgtctt cgacattgcc
    24661 gcgcggcggc tcgatgtgca gatttcacag gaagagatga acgcgcgttt acggcaatgg
    24721 gcgccgccgg cgccgcgtta caccagcggt gttatggcaa aatacgctaa gctcgtttcg
    24781 tccgcgtccc agggagctgt gacgctctag aaaacaggcg gccgggaaga aggttaacat
    24841 ggcggaatgg actttgtggg cggcactttt tactctaggc acggcgctgg cgatggtggc
    24901 gctgtacctg atctgccgcg aacgcttcca agcgatgagc actcggattg ctgcgcttga
    24961 aagagagctt gaagcggcgc gcttgcggga ggaagccaac gaaaagaact tgagcaagat
    25021 cctcgatgct gtgatggaat tgcgcggctc caccgaaagt cacttggaga atctgcgcca
    25081 tggtttggat cagttacggg cggaaaatta ctcgcaccca tctgtcgatg cggcgtgata
    25141 ccaacaataa gctttcgtgt tcgtgccatg tgatcgtgtt atgatcggtc acgcttcacc
    25201 gacaggatca cgaaactccg cgtgtcaaat tgttttcagc ctgcccatct ctatcgacaa
    25261 tctggttact tcggctgcgg tgtttgtgcc ggctttggcg gcggcgccga actggaggcc
    25321 gcggatggtt ggatatcgag aatcttgaca tcgaacagaa gcgttttgcc ggccatcgga
    25381 tgattcagat ccagcacaac ggttttttct tttatttcat gaacccgcac cgggatttct
    25441 tgcccctgtg gccctttggc ggaaagagtc gctcctagtt ttaaaccatt agccggaatc
    25501 tgctccttgg gcacctcttg aaaagccttc gcgtttacga cgccataggc atcttcaggt
    25561 ttaaccttga cccgtttttc gcctcccacc ttcatgccgg ctaattcctt ttcaagaccg
    25621 gggatgatct gcctattgcc ctgggtgtat ttcagcggat cttttccttt gttcgtttca
    25681 atgagcttgc catcttcctc cgagagcgaa tattgaagac taacgacgga accgtccttc
    25741 acgatttttt cctgtttggt tttttcggcg gatttaagaa gtgccgggga cgcgccgccg
    25801 agaactacta gagccgcggc taccgccaat aatttcatgc gcatgaatac tcctttgatt
    25861 gaatggctgc gaacaggcag ctcaggtaag gttatacacc ggaattgcgg aactcaacgc
    25921 agccccaaaa ttttctggat gaggtccgtt caatggagat gaacgattgc tgccggtcaa
    25981 gtaggatgtc aacttctatt tattgatggg ttggaaaaaa cctcgcgttg aagctcgtcg
    26041 agcaagctca tgtcgatgag atgcgccgtt tccttttcgc tctttcccag ggtcgtgata
    26101 aggttcggcc agccgtttgg ttcgttgcgc gggatatcgg caaagctatc ggcctccgcc
    26161 ctcagaagtc agccgcgatt ccggctaact tcagttgaat ccgttcctat tcgcagttgg
    26221 tatccgtgac agatcttccc gccaaattgc agcggctcgc gctgatcaat gtttgtctcg
    26281 ggcaatttat gaccgccctg gactcgcgtt cgatcattgt tgcgctaccg accatttcga
    26341 tttatttcaa tagctcgatg gcggtggtcc agtggatccc gctggcttat cagctgacta
    26401 ttattggctt tgtgcttagc ttggctcggc ttggcgacat gcggggccgc aagaagattt
    26461 acggatccgg ctttctgctg cttgctctgg gctctgcatg ttcgggtcta agcaccggct
    26521 tatggcaaat catcgcgttc agggtgttgg ccggagttgg tggggctatg gtcttggcca
    26581 atggcagggc catcctgtcg attgtctatg cccagcatga gagaggccga gccctgggat
    26641 tcgcttcgat ggctttccac ttgggttata taagcggccc ttcggttgca ggagttctca
    26701 tcgataccgt gggctggcgt tggatttttt ttctgaacct gcccgttgcg ctgacggcag
    26761 cttatatggc gcgaaaggtt ctaccggaaa ctcctatcca acgccaaacc catgcgctgg
    26821 atcttccagg aatgctcaca ctgtttgcga cggccgtaac ccttatcctg ggcttacagc
    26881 agattgccaa atccgggatt acctgggtcc cgctagcaat ctttttactg tccgtcgtgg
    26941 ccggagcatt gctgttccac ttcgagagaa aaagctctgc tccgttgctc gatctctcgc
    27001 ttttcaagat acgagtgctc actgctgggg ttctaagcca tctattcgtg gtgatctcgc
    27061 atagctcgac attctttctg ttgccatttt acctgcaggg aattctgcat ttttctccga
    27121 cccaagtcgg cgtcacggtt attttctttt ctctggcgat cgtttttcta gcgccgcttg
    27181 gcggatggtt aggagaccgc ctaggctcgc gattgctttg cacgaccggt tccgccttaa
    27241 gcctgatcag tatgcttggg ttttcccgtt tgggagcaga ttccggttac attgccgtta
    27301 tgattcctct gatgatcctg ggagtaggtt ggtcaatctt tcaggctccc aatctaagcg
    27361 ccatttttag cgcagtggag cctcgttacg taggagcagt gagcgggata tcgttaacca
    27421 gtgccaatat cgccaacgcc gtgggcgttg cgattggcag cgcgttgttt ttgcgctggc
    27481 ttaatcgtta tgggctggat cagtcgggag tgccgcccta cacgcaatgg gcagaaaacc
    27541 cggcagtctt tatcagggca ttccagaatt cctggctgct tatcgccggg ctaacggtga
    27601 ttgcgatcgt ggcttcgtcg atgcgcgggg tcgacaagcg gcagggccaa ggttcttagt
    27661 ctatattcgt gaactcttga acaaaactat ttatggcgca gcggaggttg aaagtttggc
    27721 ttgcgggcaa ctcacgttct gtaaccaccg acgagcgctc gtctgccgcc atcccacaaa
    27781 catttcacat tcctgaagcc tgcctcttgt agccaggcga gttggtcttc ttttgaagga
    27841 gtcatccgat cgaaatttag aaagagacca ccggttttga tcaacaaaaa cgtttccgcg
    27901 tagatcgatt tgatgatctt tggtgatctc acattatgga tcgctatggc tgatacgacc
    27961 gcatcgaaag gcccttcgat cttacgacgc caaccgggtt tgctgaaatc acagaggaca
    28021 tagtcgaatc gtcccgtgag atgttccata cgctgacgac cgagcttcgc catctcttcg
    28081 gatccgtcct gacagagcgc tctcccgttg ggaaaatgct tgagcagaaa ttgggctaag
    28141 gcgccgtagc ctgcgcccaa atccagtatg ttaagaggtg cttgcgaatc gtcagacaga
    28201 gttgtcgcga tcaagcgaaa gatctcctgc cgttctacct cccgttcatc ttgaccctct
    28261 gcccattgat taacgtattg ttgcgaactc cagtcatgat gatcccaagg gtcaactaac
    28321 tttgccatcg cgggtctcct tcaagatcgc ctcgcaattt acgttgataa tgcggtaaca
    28381 gggtagtcaa agtttctttg tcgagcccaa tattaactgt gccgtctaac ccagtccagg
    28441 atggccgtat tggtttcttc cggcgcttgc cagaaaaagc cgtgcgattg gccgtgtaag
    28501 attttcatca ctgctccggg aatacgatcc gcaagtatct gggattgggc gacgtgattg
    28561 ctgccactct tatcagcatc gccgatcagc actaatgtcg gcgccttcac gtcacccaaa
    28621 cgatgcgtac cctcgaaatt gtggcgcgcc atacagaggt gaataaactc cggtagcttc
    28681 gcatgagttg cccatgccaa tctgtagaac tcttcgactt tgtcgcggtt ttgcctgcga
    28741 taaacttcgg taaagaatgt gtctgattca cagatttcat tgtagataaa tttttcgaaa
    28801 cccagctcaa tcatttcgag aaccagccga tgcggcagac cgggaatgca atccgttccc
    28861 agacgcgccg ccggacctga accgctcgca gccatgatca ggcttttcac ccgacccgga
    28921 aagttctgag tcagggacaa tgcgatcctg ccgcccatcg agtggcccaa taggtgcgcg
    28981 ctggcgatct tcaaatgatc catcaaagca actatgtcca cggccatttg ctcgatagta
    29041 taaacctctt gtatcggtat ggatcgacca cagccgcgcg gatcgaaaag aatcaggttc
    29101 aaagaattcg tcagtggcat ctgcgacggc ttccaaacct cgccggaata agcggtcgag
    29161 ggcacgagaa tcaacggctc gccctgaccg tgggattcgt agtaaagatc aacaccgctc
    29221 ggcagtttta cgttcgacat aaaaatgatc ccttcaaatc aaccaagcta taagaacatg
    29281 agtcccagat ctcgatggtt gattcattga cgtcgatgtt tttctcgaag caatgagctt
    29341 ccgagatacc gctatcttgc ggccgctggt ttgtagaaac cttccttctc caactgactc
    29401 acgaaccgcg tgtctagaaa atcatcgggc ttcgctgcct tgctcttggt ttcggccgat
    29461 gccaattcac tgatgatggc ttcgagtcct tttttcgtcg catagggaac tgtctcgatg
    29521 tagctgcgtg cttcgccata cgtcgcttcg agcatatcc
//
LOCUS       DASUVS010000184         9773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1167883, whole genome shotgun sequence.
ACCESSION   DASUVS010000184 DASUVS010000000
VERSION     DASUVS010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9773
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167883"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..1289)
                     /locus_tag="VF182_10275"
     CDS             complement(288..1289)
                     /locus_tag="VF182_10275"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437506.1"
                     /translation="MAIIMQTSSLMIFLVSLIIASATEKAVFAQERIRIATAGGLSIV
                     PVWVMQDKGLLTKRGVTAEIVQIASSPVAIQAMLSGEVEAIVTSAATLVTSHLAGADV
                     TMIMSTTPTFPSVLMAAKSITEVNQLRGKAGGVNRFGSNTDFGLRLVLRKNTIDPDKD
                     VKLIQVGGTAQMVAAISRGLIQFALFNEPFVRDAEKLGFKPLVDVPSQKIPFHWNGVL
                     TKESALKARRPVFSRLTLALTEAIHVYKTEKEWTKALIGRYLKVNDTESLERAYNSLK
                     TILPETPSPTPDGVKTLLDDLAVKNSKAAAANPRDFVDTSFVEEVERSGFIKQLYGR"
     gene            1495..2457
                     /locus_tag="VF182_10280"
     CDS             1495..2457
                     /locus_tag="VF182_10280"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6437507.1"
                     /translation="MKVLITGGMGVNGAVLARSMVRDGERPVLLDNRADFSLIRDIKD
                     RVDWVEGDVTDAQFLDKVVGDFAVTHFAHLAALMPEPAEADPRLGIKVGVDGTVNILE
                     VARARNIKRVVFTSSKAVYGELLGEFGPPQYKPVDESHPKNPADLYGTIKVCCEEVGR
                     YYRNRYGIEFIALRYSTIYGPGKEARHGALSFYGQLIEKAMAGEKWVMEAGGDQLNDA
                     VYVGDVAQSVRRALTAAPSDFWQFNIGTGKGSTPREFLAAINEICPGHQIQMGSGNSQ
                     LGRSKQSFCIFDISAARKHIGYEPAYDVKKGVRDYVETIKRLRS"
     gene            2493..4295
                     /locus_tag="VF182_10285"
     CDS             2493..4295
                     /locus_tag="VF182_10285"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6437508.1"
                     /translation="MAEQSRTAGVPEVWSAGAGKKSNAQYGSDLMVEVLRELGIKYIA
                     LNPGASYRGLHDSMVNFEGKGPEIIMCTHEEIAVALANGYARVTGDIMATGLHNVVGL
                     QHASMAIFNAWCDRTPILNLGGGGPQNATHRRSTDWVHTALVQGNAVRDYVKYDDQPN
                     SVDAIPESFLRAYRIAMTEPRGPVYICLDTDVQEEKISKPMVVPHAQLFRPPSGPAAN
                     PESLRQASRLLAEAQWPVIVAGELGRNPKALPPLLDIAEELGAAVIDSDGRYAFPSTH
                     PLNLTNAREEALRDADVVLALDVPSLGVPLGPSVRERGNFAPVISPSCKVIHMTLLDL
                     ERQSWVSDAMWLLPVHVPIAADTAVALPQLLEQLRERIKSNTESARQVRERRAKIEAL
                     YNQARKKSQEWIEETWGEKPISQARFFGEINKRMQGKAWALVSAHGRRWREVIEVTEP
                     AHGIGGGRGGGVGYGLPSSIGAALGHKESGRLCVSIIGDGDFLMTSNALWTAAKYQIP
                     LLVLVMNNRSYYNDEEHQERMAKWRQRPVENKGIGIRIEDPAPDLAAIARALSVDAFG
                     PITEPDQLGSTLDKAIAIVEGGKPAVVDIITQPR"
     gene            complement(4408..4914)
                     /locus_tag="VF182_10290"
     CDS             complement(4408..4914)
                     /locus_tag="VF182_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6437509.1"
                     /translation="MGEQIGKPEPVQPQKQSQPEYLQRSKALFAEGKYEAALDLNQRA
                     LAEGRGAPDAALFNIGLISAYSQNPKKDYPKALQSFRALGAHHPQSPFAEQGNLWLLI
                     LEEHQKILEEHKKMTEERQRLSDERRAVIREREVLAQERKKLKYTEEKSRQLDLDIEK
                     RRRKSLNR"
     gene            complement(5175..6200)
                     /locus_tag="VF182_10295"
     CDS             complement(5175..6200)
                     /locus_tag="VF182_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437510.1"
                     /translation="MSLPLFWRIILGYSVILLLASGVAAYSIVQLGGLSGTARSALDS
                     ENRMMAYQETLTDAFLSEARYAGRFLLTRADAHHKEFRHFKEDFKRYMGEMTSRATAV
                     EIKARLIRTDELHLRYHELFDQEVRYIKAGQPYAQTRYQQEREKILDSALRELERLRA
                     QLQANLNNKLETMERAARAARNIAIAGTLVLIGLGVALSLLISRSITKPILELTRRTA
                     EEPQVPDGAVGTGAIPEIQELSETLAHERHRIREAARSQAEFFDDLFERVTAPLTSIQ
                     QRLTYLKRDLSQADGHEQNPSLEVIIKETERLIDHCNQLKPPSTAAMAAAGDCPEPVR
                     YQSGKAS"
     gene            complement(6204..8471)
                     /locus_tag="VF182_10300"
     CDS             complement(6204..8471)
                     /locus_tag="VF182_10300"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6437511.1"
                     /translation="MEKNALRAARAIAAEHERFFENAHQFLIMLARLPQIRENDKASC
                     RRVLAGLLEPLYADLGVTDLKGNSICTALPAASSLINSKGKHRARVIETHDFSTGTIR
                     TDASTGKTLLEIGYPVMDSPEAIRAVVIGAMNFSWVTRLAVERHLYPGASYTLINNNG
                     DVLIRYPETDDSKDEPIIAKTLLQTMASRDTEKSFEAAGANGIRRLFALSQLRNTLGG
                     QTVFAVIDIPTVAAFEEANLILVRNLIVLGVLSALALAAAWLGADIFVLRRIRDIVAA
                     TEKVAGGDLGARTTLPYERSELGQMARAFDDLAQTLEQREAEAAVSEHQINKQRQRQK
                     ILYDLNCAITSTLDMSTVLETLLQQLSSLFPLRAATISWIDRKTGALKPIVHRNLDDT
                     AAQPLPAIEEGLPIAVLNQQGLLTLSNAHLDPRTTNPEFFRKYGFVSYLGLPLIARGE
                     SLGVLSFYTTEEHRFGPEEINFLNTLVSGTAMPIYNSRLYEETRNQAAALERSNRIKD
                     EFLGVMSHELRTPLNVIMNYAEAMKIGAFGNISSEQERGTVKILSQAGHLLRLINGIL
                     EITKIESGSVILHKEQFDLAAFIAETRSDYMMPMEKKLTINWNYPTDLPAIVSDRMKL
                     KQIITNLIDNAIKFTDDGSVTVSFQLINQNHTLEIKVTDTGPGIPEDLLPLIFDKFRQ
                     IDSTTTRHFSGAGLGLYIVKVFAGLLEGTIDATSKLGEGSVFAVRVSTNLGAAPKDEQ
                     RHSAADARIMDFAED"
     gene            8954..9145
                     /locus_tag="VF182_10305"
     CDS             8954..9145
                     /locus_tag="VF182_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437512.1"
                     /translation="MEKIIEQSLMAQLAREGLEPRAGDLEEFAKIIELYLDNLKTLHS
                     VNLGAEELGPVFRPEWTGK"
     gene            9286..>9773
                     /locus_tag="VF182_10310"
     CDS             9286..>9773
                     /locus_tag="VF182_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX6437513.1"
                     /translation="MAVAEKPLYYLSIHEAQQLIQKRQLSPVELTQAVLDRIEAVEGK
                     LHAYIDLMADSAMQEARDAEAEVQKGKWRGPMHGIPVAVKDQLDVEGAQARIRQFTTG
                     VGDATAVKKLRQAGAIIMGKLHMSSLPDGSLPVPRNPWNTEHITGGSSTGSGAAVSGG
                     LCL"
BASE COUNT         2176 a         2592 c         2773 g         2232 t
ORIGIN      
        1 gaagatctcc ggcactggta tctggccatt gctgagcttt catcgaatgg cacttccccg
       61 gcgaaacgtg ccgcaaagaa ggcgagcatc gcaaagccga agaagatctc tgctaccaga
      121 ccgaagagca atacgacgat ctctccacgg tagaactttc ccaaccacac gacgctcttc
      181 tggcaccgtg cagccgaccc gtttggcgag cgaagaagaa tccgtatgtt ctcatctcgt
      241 tttcgtgatg tgaagagagt taccgggtga ttgccaccac ataaccatca gcggccatag
      301 agctgcttga taaaccccga tctttctact tcttcgacga agcttgtatc gacgaagtcc
      361 ctcggattgg ccgccgcggc ctttgaattt ttaactgcga gatcatcgag caacgtcttc
      421 accccatccg gggtcggcga gggggtttca ggtaaaatcg tcttcaagct gttgtaagcc
      481 cgttcgaggc tttcggtatc gtttactttc agatacctgc cgatcagcgc cttggtccac
      541 tctttttccg tcttgtaaac gtgaatggct tcggtcagtg ccaaggtcag ccgggaaaaa
      601 accggccgtc gagctttgag cgccgactcc ttggtcagga caccgttcca atgaaaagga
      661 attttttggg atggcacatc gacgagcggt ttaaagccaa gtttttccgc atctcgcaca
      721 aagggttcgt tgaaaagcgc gaactggatc aagccgcgtg agattgctgc gaccatctgt
      781 gcagtgccgc cgacttgaat caacttgacg tccttgtcgg gatcgatggt gttcttgcgc
      841 aataccaggc gcaaaccaaa gtcggtattg ctgccgaatc gattgacgcc gcccgcttta
      901 cccctgagtt gattgacctc tgtgatgctc ttggcggcca tcagaaccga cggaaacgtc
      961 ggcgtggttg acatgatcat ggtaacgtcg gctccggcca ggtgcgaagt caccaaagtg
     1021 gctgccgagg ttacgatcgc ttcgacctct cccgagagca tggcttgaat ggccaccggg
     1081 ctcgacgcga tttgcacaat ctccgcggtc acgccgcgct tcgtcaacag tcctttgtcc
     1141 tgcatcaccc agaccggaac aatcgagagc ccaccggcag tggcaatacg gatgcgctcc
     1201 tgagcgaaca ccgccttttc ggtggcgctc gcgattatca gtgatacaag aaaaatcatt
     1261 agcgaggatg tttgcatgat gatggccatc tttgtctctg aactaagaac caaattcgtc
     1321 gctatctgga gcttataaac agcgaccttc cacaagtcaa aagtatttcg cggggagcct
     1381 gcgcaatagc acgattctga aatgtcttct attcaattga aatgtgaatg tgctatagcc
     1441 catgctaata gatactgaga tgtcgatgaa cggtcgcatg aaaggaatcg ctcgatgaag
     1501 gttttaataa cgggcggcat gggagtgaac ggcgcggtgc tggctcgaag catggtccgc
     1561 gacggcgaga gaccggtgtt gttggacaat cgggcggatt tctcgctgat tcgcgatatc
     1621 aaagatcgag tcgattgggt cgagggcgac gtcaccgatg cgcagttttt ggacaaggtg
     1681 gttggtgatt ttgctgtgac tcattttgcg cacctggccg cgcttatgcc ggaaccggcc
     1741 gaagccgatc cacgcctcgg catcaaagtc ggggtcgacg ggacggtaaa catcctggaa
     1801 gtcgcgcggg cgcgaaacat caagcgggtg gttttcacca gctccaaggc tgtatacggt
     1861 gagctgctcg gcgaattcgg accgccgcag tacaaaccgg tggacgaaag tcatccgaag
     1921 aatcccgccg acctttacgg cacgatcaag gtttgctgcg aggaggtcgg tcgctattac
     1981 cgcaacaggt acggcatcga atttatcgcg ctacgctatt cgaccatcta tggacccggc
     2041 aaagaagccc gccacggcgc tctgtcgttt tacggccaat tgatcgagaa ggcgatggcg
     2101 ggagagaagt gggttatgga agccggcgga gatcagctaa acgatgcggt ttatgtcggc
     2161 gatgtggcgc aatcggtgcg ccgcgcgctt actgcggcgc cgtcggattt ttggcagttc
     2221 aacatcggca ccggaaaagg gtctacgccc cgagaatttc tcgcggcgat caacgaaatc
     2281 tgccccggcc atcaaattca aatgggcagc ggcaacagcc agctaggacg cagcaagcag
     2341 agcttttgca ttttcgatat ttccgctgcc agaaagcata tcggttacga gcccgcctat
     2401 gacgttaaaa aaggcgtgcg cgattatgtc gaaacaatca agcggctgag atcatgagca
     2461 acaagcttag gcattacatt acggaggaat gaatggcaga acaaagcaga acggcgggcg
     2521 tcccggaggt atggtccgcg ggcgccggca aaaaaagcaa tgcgcaatac ggctcggatc
     2581 tcatggtcga agtgctgcgc gagctgggaa tcaaatacat cgccttaaat cccggcgcca
     2641 gttatcgcgg cctgcacgat tcgatggtga atttcgaggg caagggtccg gagatcatca
     2701 tgtgcacgca cgaagagatc gccgtggctt tggccaatgg ttacgcccga gtcaccggcg
     2761 atatcatggc caccggcctg cacaacgtcg tcggcctgca acacgcgagc atggcgattt
     2821 tcaatgcctg gtgcgaccgc acgccaattt tgaatctcgg cggcggcggc ccgcagaacg
     2881 cgactcatcg ccggtctact gattgggtcc acacggctct ggtgcaaggc aatgccgtgc
     2941 gcgattacgt caagtatgac gatcagccaa attcagtcga cgccattcct gaatcattct
     3001 tgcgcgccta ccggatcgcg atgaccgagc ccagaggccc ggtttatatc tgcctcgata
     3061 ccgatgttca ggaagagaaa atcagcaaac cgatggtggt gccgcacgcc cagttatttc
     3121 gcccgccgtc aggtccggca gccaatcctg aatccctgcg gcaggcatcg cggctgctcg
     3181 ccgaagcgca gtggccggtg atcgttgccg gcgaactcgg ccgcaatccc aaggcgctgc
     3241 cgcctctgct ggacatcgcc gaggagctcg gcgcagcggt aatcgattcg gatggccgct
     3301 atgcatttcc cagcacgcac ccgcttaatc tcaccaacgc ccgcgaggaa gcattgcgcg
     3361 atgccgacgt cgttctcgcg ctcgatgtgc cgagtctcgg tgtgccgctt ggcccttcgg
     3421 tgcgcgagcg cggcaatttc gctccggtga tttcgccgag ctgcaaagtg attcacatga
     3481 ccctgctcga tctcgaacgg cagagttggg tgagcgacgc catgtggctc ttgccggtgc
     3541 acgtgccgat cgcggccgat accgcggtgg cgttgccgca gctgctcgag cagttgcgcg
     3601 agcggatcaa gagtaatacg gaatcggctc ggcaggttcg cgagcgccgc gcgaaaatcg
     3661 aagctctcta taatcaagcg cgcaagaaga gccaggagtg gatagaggaa acctggggcg
     3721 aaaaaccgat ttctcaagcg cgcttctttg gcgagatcaa caaacgaatg caaggcaagg
     3781 cgtgggcgct ggtatcggcg cacgggcgcc gctggcgtga agtgatcgaa gtcacggagc
     3841 cggcgcacgg cattggcggc ggcagaggag gcggcgtcgg ctacggcttg ccgtcatcga
     3901 ttggcgccgc gctcgggcac aaggaaagcg gcaggttatg cgtcagcatc atcggcgatg
     3961 gcgactttct gatgacctcg aatgccttgt ggacggcggc caagtatcag attcctttgc
     4021 ttgtgctggt catgaacaat cgctcctact acaacgacga agaacatcag gagcggatgg
     4081 ccaaatggcg gcaacggccg gtggagaata aagggatcgg cattcgcatc gaggatcccg
     4141 cgccggatct ggcggcgatt gcgcgcgctt tgagcgtcga tgctttcggc ccgattaccg
     4201 aacctgatca actcggctcg actctagata aggcgatcgc gatcgtcgag ggagggaaac
     4261 cggcggtcgt ggatatcatc acgcagccac ggtgagctgc gatcagcggc gacggctcga
     4321 tagttcggta aacggaagga tccgagttca gacttataga tgggaagagc gcgtctattc
     4381 ggcttggaag gagaggtgcc ctctggctta tcggtttaaa gattttctcc gcctcttctc
     4441 gatgtccaga tcgagttgac gcgatttctc ctctgtatac ttgagcttct tgcgttcttg
     4501 cgccaacacc tctctttccc ggatcacggc tcgcctttcg tcagacaacc gttgtctttc
     4561 ctcggtcatt ttcttgtgtt cttccaggat cttctggtgt tcttccagga tcaggagcca
     4621 gagattcccc tgttcggcaa acgggctctg cggatggtga gcgcctaggg ctctgaacga
     4681 ctggagtgct ttgggatagt ctttcttggg gttttgagag tacgcggaga tgagtccgat
     4741 attgaacagc gccgcatcgg gtgcgcccct accttcggcg agcgctctct ggtttagatc
     4801 cagggccgct tcatacttgc cttcggcaaa gagagcttta ctgcgttgca aatattccgg
     4861 ctgcgattgt ttttgcggct ggacgggctc gggtttcccg atctgttctc ccattgtagt
     4921 gcaacctatg actgacacgc atgcagccaa ggccgagatg cttaacgaac gatgagaaag
     4981 tctcatggtg cctctgcaag tccggcttga tcggcgatgt tgttcatagt ttctttgcct
     5041 ttccagggag gtacgattta atcgactgaa tgccggccat aatgcaagca aaacgcgacc
     5101 gagcttaacc gctaaccgca gtgtatgacc cgcgcgcgcg aaaagttcgc tccctgagag
     5161 gatcattttt ttggtcatga tgctttcccg gattgatacc gcaccggctc gggacaatcg
     5221 ccagcggcag ccatggcggc ggtagatggc ggttttagct ggttacaatg gtcgatcaat
     5281 cgctcggttt ccttgataat gacttccaat gatgggtttt gttcgtgccc gtcggcttgc
     5341 gacaaatccc gtttcaaata cgtgagcctt tgttggatcg aagtaagcgg ggccgttacc
     5401 cgctcgaaga ggtcatcgaa aaattcggct tgtgatcggg cggcttcacg tattctgtgt
     5461 cgctcgtgtg ctaaggtttc tgagagttct tggatttccg ggattgcgcc ggtcccgacc
     5521 gcgccatcgg ggacttgggg ttcttcggct gttctccggg tcaactccaa gatcggcttc
     5581 gtgatgctcc tgctgatcag caaagacagg gcgacgccca gtccgatcag gaccagagtg
     5641 ccggcaatgg caatatttcg cgccgcgcgt gcggctcgtt ccatggtttc cagcttattg
     5701 ttcaggtttg cttgcagctg cgctctcagg cgttcgagct ctctcagggc ggaatctaaa
     5761 atcttctctc gctcctgctg ataccgggtc tgagcatacg gctggccggc ttttatgtaa
     5821 cgaacctctt gatcgaacaa ttcgtgatag cggaggtgca actcatcggt tctgattaaa
     5881 cgcgccttaa tttcaacggc ggtggcgcgt gacgtcatct cgcccatata tcgcttgaag
     5941 tcttccttga agtggcgaaa ctccttgtgg tgggcatcgg cccgggtaag taaaaatctt
     6001 ccagcataac gcgcttcgga gaggaaggcg tccgtcaatg tctcttgata tgccatcatg
     6061 cgattttcgc tatccaaagc cgagcgtgct gttccactca ggccacctaa ctggacaatc
     6121 gaataagccg ctacgccgga ggctaggagc aagatgacgg agtatcctaa aatgatacgc
     6181 cagaataatg gcaacgacat atcctaatcc tccgcgaagt ccataattct cgcatcggct
     6241 gccgaatgcc gctgctcatc tttgggcgcc gccccaagat tcgtggacac gcgcacggcg
     6301 aagacagagc cttcgccaag tttgctggtg gcatctatcg taccttcgag gaggccggca
     6361 aagactttaa cgatgtacaa accgagcccc gcaccggaaa aatgccgcgt cgttgtactg
     6421 tcgatttggc gaaacttgtc aaaaatcaat ggcaggagat cctcgggaat tcccggtccg
     6481 gtatctgtga ccttgatctc gagagtgtga ttctgattaa tcagctggaa agacacagtg
     6541 actgatccat cgtcggtaaa ttttattgcg ttatcgatca agttggtgat gatttgtttc
     6601 aatttcatac ggtcgctgac gatcgcagga aggtcggttg ggtagttcca attgatggtg
     6661 agctttttct ccatcggcat catatagtct gatcgggttt ccgcgatgaa tgctgcgaga
     6721 tcgaactgtt ccttgtgcaa gatgaccgat ccgctttcta tcttggtgat ctccaaaata
     6781 ccgttgataa gcctgagcag gtgccctgcc tgggaaagta ttttgaccgt tcctctttcc
     6841 tgctcgctgc tgatgtttcc gaacgcgcct attttcatag cctcggcata gttcatgatg
     6901 acatttagcg gagtcctcaa ctcatgggac atcactccaa gaaactcatc tttgatgcgg
     6961 ttcgatcttt ccagtgccgc cgcttgattg cgggtttctt catagagccg cgaattatag
     7021 atgggcatgg cggttccact gaccagggtg tttagaaaat tgatctcctc gggcccaaat
     7081 cgatgctctt cagttgtata gaaagaaaga actcccaatg attcccctcg ggcgatcaaa
     7141 ggcaaaccga gataagagac gaaaccgtac tttctgaaga attccgggtt ggttgttctt
     7201 gggtccaaat gggcattcga taaggttagc agcccctgtt gattgagcac ggcgatcgga
     7261 agaccttctt cgattgccgg cagcggttgt gccgccgtgt catcgagatt gcggtgaacg
     7321 ataggcttta gcgcaccggt ctttctgtcg atccaactta tggtcgcggc gcgaagcgga
     7381 aacaagctgg atagttgctg caacagagtt tccaataccg tcgacatatc gagagtagag
     7441 gttatcgcgc aattaaggtc atagagaatt ttttgccgct gtcgttgttt gttaatctga
     7501 tgttccgaca cggcagcttc ggcttcacgt tgctccaacg tctgcgcaag atcgtcaaaa
     7561 gcgcgcgcca tctgtccgag ctcgctccgc tcgtagggca gcgtggtacg cgcacccagg
     7621 tcgccgccag cgaccttctc tgtcgccgca acgatgtctc ggattcggcg aagaacgaaa
     7681 atgtcggcgc caagccatgc ggccgccagc gctagagctg ataacacccc gagaacgatt
     7741 aggttccgaa ccaaaatgag attcgcctcc tcgaatgcag cgacagttgg gatatcgatg
     7801 accgcgaaaa cagtttggcc gcctagtgtg tttctcaact ggctcaaagc aaagagtcgc
     7861 cgaatgccgt tggcaccagc tgcttcgaag cttttttcgg tatcccgaga agccatggtt
     7921 tgtagtaaag tttttgcgat aatcggctcg tccttcgagt cgtctgtttc agggtatcga
     7981 atcaacacgt ctccgttgtt gttaatgagc gtgtaggatg cccccggata cagatgccgt
     8041 tcgactgcca agcgggtcac ccaagaaaag ttcatagctc cgatgacgac ggcgcgtatg
     8101 gcctcgggcg agtccattac cggatagccg atttccaaaa gtgtcttacc ggtcgaggca
     8161 tcggttcgaa tagtgccggt ggaaaaatca tgagtctcga tgacccgcgc tcgatgtttt
     8221 cctttgctgt ttatcagtga gctggcggcc ggcagcgctg tgcaaatgga attgcccttg
     8281 agatcggtga caccaagatc ggcgtaaagc ggctccaaca atcctgccag gactctccgg
     8341 cagcttgcct tgtcgttctc gcgaatttgc ggcaagcggg ccagcatgat caagaattgg
     8401 tgggcgtttt cgaaaaacct ttcgtgctca gcggcgattg cccgggccgc tctcaatgcg
     8461 tttttttcca cttgctctgc ggtcaaatca cgatgactag aagcgctgtg cagaatgacg
     8521 ccaaaggctg gaatgacagc caacaataca agaacaataa gcctgcatcg taacgatgaa
     8581 atgaactgaa ccatggaggc gaaccggtgg acgaaggcgg cgagtggccc gcgttacact
     8641 cgaggaccgc aaccggcatg ccagtcagtc tatagcgcgt tttggagcgc aactataaac
     8701 gatggcaacc caacccactc acatcacaaa aagcagaaca actttgccaa ttcctatgat
     8761 atcttgcgtg cgagcgtcag gtgtgaataa tcctgcatta atttgttctg ctggaggaca
     8821 cggcaaacca caatcagcgc cgcgtgcgtc ttctactcct aagaatggaa gaacttgact
     8881 caaacgcgcc atgaacggta gagtcgcgac ttcaaccttc cggaccgtca aacagctctg
     8941 aaggagaaag catatggaaa agatcatcga gcaaagcctg atggcgcagc ttgcgcgcga
     9001 aggtttggag cccagagcgg gcgacctcga agagtttgcc aagatcatcg agctttacct
     9061 ggataacttg aagactttgc attcggtcaa tctcggcgcc gaggagttgg gcccggtgtt
     9121 tcggccggag tggacgggaa agtgattgaa tgactgacac ctgatgtctg gcttgcttgg
     9181 aacgacaagc aaagccggcg caccagagtt gttaccgaaa caaagtcaag gatccgtttt
     9241 tgtcacgcaa ttagttgaca ccgcaaattg caaaggaggc tcgccatggc tgtagcagag
     9301 aagccgctct attatctgtc gatccatgaa gcgcaacagc tgattcaaaa acgtcaactc
     9361 tcgcccgtcg agctgaccca ggctgtgctc gatcgcattg aagccgttga gggaaaattg
     9421 catgcctata tcgatttgat ggccgacagc gcgatgcagg aagctcggga cgccgaggcg
     9481 gaagttcaga agggcaaatg gcgcggtccg atgcacggca ttccagtggc cgttaaagat
     9541 caattggacg tggaaggggc ccaggcgcgg atccgccaat ttactaccgg cgtaggcgat
     9601 gcgaccgctg tgaaaaagct gcgccaagcc ggcgcgatca tcatgggcaa gcttcacatg
     9661 agcagtttgc ctgacgggtc gctgccggtg ccgcgcaatc cgtggaacac cgaacacatc
     9721 accggcgggt cgagcacggg ttccggcgct gccgtctccg gcggactctg tct
//
LOCUS       DASUVS010000185        31268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_512463, whole genome shotgun sequence.
ACCESSION   DASUVS010000185 DASUVS010000000
VERSION     DASUVS010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 31268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31268
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512463"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VF182_10315"
     CDS             complement(<1..807)
                     /locus_tag="VF182_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6437514.1"
                     /translation="MTDRSDYRNFFPGQRRTRLPGGYMGKILRVDLSTEKLVDLQLPE
                     EPILRKYWGGQLFAEYVLLNELPINIDPYDARNVIVGMTGPITGTGFTPGGTKMCFVY
                     LSPATRYTLGRGATSGYFGTSLKAAGYDGVIITGAAQQPTYLYIGEDKVELRDAGRVW
                     GKGSRDTEDMLRDESGHQDARVGCIGPAGESLIRAAMFVNDYNHNAAHGLGAVMGSKK
                     LKAIVAWGTKRPRVHDKNTLIEAGLRWRKAIAARTTTVKKRLTSVGHGEDW"
     gene            complement(1016..1987)
                     /locus_tag="VF182_10320"
     CDS             complement(1016..1987)
                     /locus_tag="VF182_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437515.1"
                     /translation="MNEGVRLYAGTQAGLIIWRSVARGWQEVGRHFEDGIIDSVFGCK
                     TMPERVFVGVTHDGLYRTTDGGESWAKVLDGDIRSVAVDPTDDKVIYSGVEPVALYRS
                     EDGGNHWQELTTLQDVPETARKNWWFPQPPHKGHIRNIFIHPDNPSTIYLCIEHGGIV
                     RSFDHGKSWEDVSKGIDYLDIHVIANLPKRMDRYYVATARGFFTSDKPEDGWLRAENG
                     LTRNYFHDFLFLPPVISGEKPTMILATADQSPGFWRRENRGARAALFKSADGAGSWTR
                     ITDGIADDLDSMIWALVHHPTDHRALFAGFGNVARAMPVDQETPATS"
     gene            complement(2157..2885)
                     /locus_tag="VF182_10325"
     CDS             complement(2157..2885)
                     /locus_tag="VF182_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6437516.1"
                     /translation="MTPEKDLREQLATCTRIFAMQGLIGLFGHVSVYQPGTKTIFVTP
                     GSGSDKPRFQASDMIPIHLDGKPLDGKTLPPVEWPIHTALHGARADALAVAHLHTAYA
                     TLFTIARREFHPVTLQGTIFGDGVPLYKEAHLVTTPQRGDNLLKTIGNHRAVLMRGHG
                     IVVAGKSLAEVLFASMILEDDAKKTMQALTLGPVDHFSAEECRGFNAEIDFERRSQRA
                     WDFFTRLESTWDRQAGTGRMELFP"
     gene            complement(2882..3898)
                     /locus_tag="VF182_10330"
     CDS             complement(2882..3898)
                     /locus_tag="VF182_10330"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437517.1"
                     /translation="MRPSKFRSSFIARPWILSALLMVSFTTEVCAEKIRTAIPQANLN
                     YLSIYVADAKGFFGDEGLENETVVISGPLATAALLSGDVDFSGGGGSGMRAALKGAPV
                     KGIFFQTEKVTFYLIADPSIKTAADLKGKKVAVGSSNDTQDRMITMFAERGGVSSRDL
                     TRIAMGSDTNSRILALKSGAAHASAVDPGGLVFAQKEGLVSLGFLGDLFPMPFQGFVT
                     LDRKIRENPRQIKRWLKAAIRGLMFVRDQPEEAVNLGIKKLQLGKATRPMIVEGTKNY
                     ARALPVGVPGLPTAEGIRNFLEYDIKIPLQIREDIPPDRLLHLQLVEEVKKELEAAQR
                     RPSR"
     gene            complement(3967..4779)
                     /locus_tag="VF182_10335"
     CDS             complement(3967..4779)
                     /locus_tag="VF182_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6437518.1"
                     /translation="MRDSLTGPGESIQQYGNLVPTGRLHDGNARSILFDATKCIGCRH
                     CVQACKDWNDHDRTNLYSLSSTNWITMEPPVLEGLSPLWARNSCMHCEFAACAAVCPV
                     EAITKYEEGPVVINQEICIGCEYCIHACPWEVITKDKVTQKASKCTMCSDRISEDKQP
                     FCVQACPTQALDFGLTEEIAAKARKQAGTVGGYTYGDKEAGGGNVIYVLKENKEKYGV
                     RSIGEEKFPKHKIPIGLMLRDLFTPRCGIKGKLRAFYLAIIHPKRLIYRYFS"
     gene            complement(5014..5607)
                     /locus_tag="VF182_10340"
     CDS             complement(5014..5607)
                     /locus_tag="VF182_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HEX6437519.1"
                     /translation="MAGKNTAAFGIYPNETALEDGISALQQAGYRNEDISVLFPENEG
                     TKDFAHRKGTKAPEGAASGAGTGAVAGGVLGWLAGIGAIAIPGVGPFIAAGPIMAALA
                     GVGVGGALGGLTGALVGAGIPEYEAKRYEGRIKDGGILVSVHCDNSEWTSRAKDILER
                     TGAQDVSSTSEASADFAETDKPLPRDASGTVRRRDDV"
     gene            5855..7093
                     /gene="glp"
                     /locus_tag="VF182_10345"
     CDS             5855..7093
                     /gene="glp"
                     /locus_tag="VF182_10345"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX6437520.1"
                     /translation="MAQTFFKVLAPQEALRLLVQVNPVPTERVATVTARARVLSEDLR
                     SAVDLPHFNRAAMDGYAVRSKDTFGASQSLPAYLKLAGVVEMGKEAIQTVQTGEALRI
                     STGGMMPPASDAVVMVEYTNEVEGGLVEVHRGVSPWQNVIQIGDDIKKGEPVFQSGRR
                     LKAHDIGALTGIGLSSVPVFKRPRVALISTGDEIVDADVTPLPGQVRNINQHSLAGLI
                     AECGAELRDFGVIPDDRERLTSAITQALDWADLVLLSGGSSMGAKDIALETILSFPDS
                     EFIFHGISVAPGKPTIFAKACGKPIMGLPGYPVSALVIFDLFAAPLIRRLGGEVIATH
                     ARFARTAKATLKTNIASQIGREDYVRVALEQETQGLLATPLPSKSGAIFTLVKADGMV
                     RIDLNQDGLEAGEEVEVILF"
     gene            7197..9125
                     /locus_tag="VF182_10350"
     CDS             7197..9125
                     /locus_tag="VF182_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HEX6437521.1"
                     /translation="MARKRYLKKTPLKEAKEFFLAKVEPSRIGNEFISVEEALDRITA
                     EPVFAKISSPHYHAAAMDGICVRAEDTFGATEFKPKTLICASSDSPGPNAFHYVDTGN
                     SLPAWANAVIMIEKVRQLKDGSLEIFESVGPWNHVRLVGEDVVATELLLPRYHRLRPY
                     DLGALLASGHTLVEVKSRPKVGIIPTGDELIAPGEQPLPGAVIEFNSTILAAFLREWG
                     AAPIKYPRVKDQAVLLEEVVRRAAAECDLVTIIAGSSAGEHDLTADVVADLGELWAHG
                     IDVMPGKPAVLAGVADKPVIGIPGYPVSAIVIAREILRPAVEKFLGAAAPSYSIVHAV
                     VPKKIASHLGLEEFIRVSVGRVENKLVAVPLARGAGVITTMVRADGLLRIPSLVEGLN
                     GGEEVEIELLRPRADIENTILCTGSHDLAIGVIEDQLKQLYPELKIAAANVGSVGGLL
                     ALQRGETHMAGTHLLDPNTGVYNVPDIKRTIPKLPVALVHLARREQGIVVARGNPKSI
                     GNLSDLTSGIRFVNRQSGSGTRVLLDFELKRLGINPNSINGYDREEFTHMSVGVAVAS
                     DLADAGLAVRAAAQALGLDFIPIASEQYDLLFSRSFFESDRGERLMLVLQSRAFKDAV
                     SALGGYDSSGSGKLLYQQ"
     gene            9437..9655
                     /locus_tag="VF182_10355"
     CDS             9437..9655
                     /locus_tag="VF182_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6437522.1"
                     /translation="MDEKIDSIRLLTLDEAAALLQVSKRTLHRMIKTNELPAFKVGGQ
                     WRVRETQLRQWVEHREAGSTERERKGNL"
     gene            9762..13520
                     /locus_tag="VF182_10360"
     CDS             9762..13520
                     /locus_tag="VF182_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012412573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6437523.1"
                     /translation="MPKTKSLAKPRSFARATSSGFLQSFWEFLPSGESLPPAVWQSRH
                     LFLLRLTWLHALLLLLIAVLYGESWEFSLPAVVRNGTVLQTVGNAVIVGLFASVAALT
                     SGRALPAAAVGFGLLSASAFLVHLSGGAIEFHFHFFVVLAFLALYQDRVSYVLVLVYL
                     VIYHFVVGAIWPQHVFNHTAALAAPWSWAAIHSFFLIAAGIGNMSAWRFNQKALAQIN
                     LILNSATEGIYGIDLEQRILFMNSAGAELLRCRPDQAIGKTLQQVLRHTRSDGTAFSN
                     QPSSIVASLRDGAKHQASDELFWRSDGSSFPVEYKCAPIFDRNALAGTIVTFSDISAQ
                     QQRETALRQSEERFRQIAENIKAVFWITNPMNNEKLYVSPAYREIWGRDTAKIGSLSQ
                     SWLGLLHEDDHSQVLEAVGTKQVTGQYNEEYRIVREDGSIRWINDRGFPVRNESEEVY
                     RIVGLAEDITARKQAQQDLLDRYTEMNVLHNVGQAVLSATDIELLLENILEQALATLS
                     LEIGNIRLFEPISGTRIGIYRGYGNQENTRVYADMKNPRSDALSMRIIESGKSLVFEN
                     LAARDGLPAFTKEQVCSALVVPIILERETLGLIEVGSRKRWTFRPQDMRLLNAIGNQV
                     GLAIQKARLLHETERRANEQAALNIIAKAISQSLRIDELLKIALEKIFEVTGRERLLI
                     RLKESASNGMILAAHRGFLPEEIEEFAKASSDAITGEVFASGQPLVINNSPAIGSPKA
                     ISPENPAGMIPIKAGTQVMGILRVSAVRPIPFELRELELLEAIGNMLGVALENARLFS
                     ETEARYRELKTLQAISDTILETLDLTIMMERILDRAFEIGRFDCGVIRLLDRDHESLK
                     LVASRGYRDREGVLDHYEASEQKSTARIIAEVMASKQTNVKNLSTSDGMRTFRREGVC
                     TVVTIPLHTQQDVLGIIQLGSRITREFQDSELRIFDAIGGQAGIAIQKARLYDESQRA
                     QAALNEKAAELTRSNTDLHHSTQEVKSAKEKLERVNSLLTVQAAELARSNTELEQFAY
                     VASHDLQEPLRMVASYVQLLARRYKGKLDSEAEEFIGFAVDGSKRMQDLIQALLAYSR
                     IGTKGRQFAPTDCEIVLQNALKNLQIAIEDSRARITHDPLPTVMGDAIQLGQLFQNLI
                     GNAIKFRGEKLPSVHVTAERHDREWLFSFRDDGIGIDSQYAERIFVIFQRLHTKEEYP
                     GTGIGLALCKKIVERHSGRIWVESEPHKGSIFRFTLPAENPVEGDKPL"
     gene            13517..13969
                     /locus_tag="VF182_10365"
     CDS             13517..13969
                     /locus_tag="VF182_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013936054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6437524.1"
                     /translation="MNVYNGCIQVLLVEDNPGDVRLTKEALKEGKLLNQLTVVGDGVE
                     ALSFLRKEGVYADAPQPELILLDLNLPKKDGREVLAEIKADPNLRRIPVVVLTTSSSE
                     EDILKIYDLHANCYITKPVDLEQFMGVVKSIEDFWVSVVKLPSYEATK"
     gene            13966..15603
                     /locus_tag="VF182_10370"
     CDS             13966..15603
                     /locus_tag="VF182_10370"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6437525.1"
                     /translation="MSSAPIQVLLIEDNPGDARLLRELLQETGSSQFELVHADRFSEA
                     LAWLSERRFDVILLDLSLPDAQGLDTIGRLGAQAEGTPIVILTGLNDEELAIRALQQG
                     AQDYLVKGQADGQLLARALRYSIERHKAEESLKARNRELMVLRKISEAILASLDLNTV
                     LEHILEQAIFIGSFDLGNIRLLESDGDTLQVVVVRGYRDLGNVASHRAISRNTGIAQS
                     SHFKELVFQQPCVEEAVQSCDGLKTLKKEGVQSFVQVPVRADGEVLGIIQLATRTPRK
                     FKVEEVSLWETIGNHVGVAVQRAQLYEETKRQARELEKASKLQADFSAMIAHDLRSPL
                     MNITGVTEVMLDGMFGPVTEEQKKWLLRVQANSHNLIDLVSDFLDFSKLESGYVNVSK
                     QVVSVTDLVRKSIESFQVVAQNKQVTVAEAIDASLPSILADPQRLEQVLSNLISNAIK
                     FTGDGGEVKVGAALTATGGIRVWVQDSGEGIPREEIGELFQKYRQGGNANSSNHKGTG
                     LGLVICKMIVEAHGGTIWADSQEGQGSIFSFSLPVAA"
     gene            complement(15629..17182)
                     /locus_tag="VF182_10375"
     CDS             complement(15629..17182)
                     /locus_tag="VF182_10375"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX6437526.1"
                     /translation="MAKSRDDLEKKLSALEKAAVAGTVKATGKQPDQATLTARERVET
                     LLDPGTFVEEFMLAETQCTDFGMAERKQPSDGVVTGFGKVDGRPVYVFSQDRSIMAGS
                     VGSAHAEKIAYVIQTARRLGVPLIGLNDSAGARIQEGLSVTSAIGKIFFENSIASGCI
                     PQISAIMGPCIGVGSYSPALTDFVLMVQNSSQMFITGPAVIKEVLGETVTMEELGGAK
                     IHSEISGVADLVVKSDQECLTTIRRLLGFLPPNFESLPPRKSTSDHPDRSLDALNKIV
                     PDDMRRAYNILQVIKAIVDDNDFLELKPKFARNLVTGFGRLDGYPVGFVANQPMFLAG
                     SLDVDASDKAARFIRFCDAFNLPIITLMDVPGFFPGRQQEEKGIIRHGAKMLYAYAEA
                     TVPKITVVLRKGYGGAKQALCTREMGADQLFVWPGVELAVMGGGGAVNVLYKREIERS
                     ADPEQTRREKIAEFQERFSGPFEALSKQFAHAAVHPAQTRQRLIRSLEILRNKKEERP
                     KKKHGLMPV"
     gene            complement(17273..18418)
                     /locus_tag="VF182_10380"
     CDS             complement(17273..18418)
                     /locus_tag="VF182_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEX6437527.1"
                     /translation="MESAIASGLQGLLLGISNLTLGHAVMILIGSLLLYLGISKGYEP
                     LLLLPIGFGAILVNIPLAGLMDEHGILRYFYENGILTEIFPVLIFLGIGAMTDFSPLL
                     ENPKIILLGAAGQFGIFLTLLLALAVGFDKLDAVAVAIIGACDGPTAIYVSSKYAPHL
                     LGAVSVAAYSYMSLVPLIQPPIMRMLTTERERKIVMGVIRQPVSKTTKILFPVVVTIF
                     ASILAPIGTPLIGTVMLGNLMKESGVVERLKTASENEIANIITLLLGLTIGATMEADK
                     FLRGETLLVLGLGLVAISLDTVVGVLFGKLMCLLTGGKINPLIGAAGISAFPMAARVA
                     QTEGQKYNKKNYLLMHAMSANAGGQIGSVIAAAVMLSVLTGMGIVGG"
     gene            complement(18427..18573)
                     /locus_tag="VF182_10385"
     CDS             complement(18427..18573)
                     /locus_tag="VF182_10385"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HEX6437528.1"
                     /translation="MDIWTFGWTLSIIGMAGTMLVLWCISLLILLLRKMFPYKPEATA
                     PKKA"
     gene            complement(18624..18839)
                     /locus_tag="VF182_10390"
     CDS             complement(18624..18839)
                     /locus_tag="VF182_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEX6437529.1"
                     /translation="MATPITAPMVGKILKLEKNAGDRVDEDEVVLVMEAMKMEIPIVA
                     PASGVLKEIKVSVGQAVEADQLLAEIA"
     gene            19102..20196
                     /locus_tag="VF182_10395"
     CDS             19102..20196
                     /locus_tag="VF182_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia lyase-activating protein"
                     /protein_id="HEX6437530.1"
                     /translation="MAKPAVQQDVSIDNIPYYEKWQLGEGIPIVKTFFVQDLRKVELK
                     PWDRAGGNGAFINMEGAEGTTGAYVLEIPAGKSLNPQRHLYEDMLYVLKGRGATTIWN
                     DKGAKQTFEWQEGSLFALPLNSWYQHFNGQGDEPARFFSVNSMAITFNLFHNEDFIFN
                     TPRDFMDRFDGDPEYFSGKGKAHPGRVWDTNFIADARNFKLEDWKERGASGKNVMLEM
                     ANGSMAAHISEFPVGTYKKAHRHGPGFNVMIIKGHGFSLFWRQGEQPKRYDWQDGSVF
                     TPPAMMFHQHFNTGATGARYLPPRLGGIKYSLGESFGDITKVDKDVKAGGNQIEYYDE
                     DPNIRKMFEEELAKSGTTTRMNPDFYKKPA"
     gene            20325..20915
                     /gene="plsY"
                     /locus_tag="VF182_10400"
     CDS             20325..20915
                     /gene="plsY"
                     /locus_tag="VF182_10400"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX6437531.1"
                     /translation="MEILLIVLGYLLGSVPVGFIVGLRSGIDVRETGSGNVGATNVAR
                     VLGKRQGILTLVADTAKGFLPVIVAMQLGASLSATILVGTAAFLGHLFPVFLKFKGGK
                     GVATALGVFLALAPIATLVLVALFAVTVLASRIVSLSSILTAVAAPIIVWLFSYPPLV
                     VGMSAFIALAITCRHRSNIRRMMNGSEPRFGSASSQ"
     gene            complement(20902..21612)
                     /locus_tag="VF182_10405"
     CDS             complement(20902..21612)
                     /locus_tag="VF182_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX6437532.1"
                     /translation="MNNLPRFFTAALIALAMIGAGCSGGSLTTREKGAGIGALGGAAA
                     GGIVGAAVGRPGVGAAVGGALGLGTGALIGDQLQGQEIKQAEQQKAIDQQRAEIAKNQ
                     ALIDELKKRNIEARETSRGVTVNLPNVNFQFDSADLTVDGRDRVNQIANILQREAPNR
                     KVTVEGHASRESAAQEAYNQRLSERRASSVAQSLERNGVNGGRLTSAGLGTRAPIASN
                     DTEDGRRQNRRVEVIIEN"
     gene            complement(21641..21979)
                     /locus_tag="VF182_10410"
     CDS             complement(21641..21979)
                     /locus_tag="VF182_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEX6437533.1"
                     /translation="MKRALTITVGFCLISAVVGCSSGGLTTREKGAGIGALGGAAVGG
                     IIGSAVGRPGAGAAIGGALGLGSGALVGDQLQGQEQKQSEQQRQIDANQAELERQKRE
                     LERLKKTAEY"
     gene            22192..23292
                     /locus_tag="VF182_10415"
     CDS             22192..23292
                     /locus_tag="VF182_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX6437534.1"
                     /translation="MESLSQERILGVLRKVQDPELHRDIVSLGMVKNLVLNDGKVSFT
                     VELTTPACPLRETIETDCKKALAEVAGIRDLEINFGAQVRGSKAGEGQTDLLPSVKNV
                     VLVAAGKGGVGKSTVAANLAVALKKHGAATGLLDADIYGPSVPIIMGVKGEPSKVEVN
                     GAFKIAPTIAHGVPVMSIGFFLDPEQAVIWRGPMLGKALHQLMADVHWGELDYLVVDM
                     PPGTGDVQITFSQQLKVSGALLVATPQDVALADVIRAKTMFDKVMIPIVGVVENMSYF
                     ICDGCNKKHEIFSRGGAFRAAERFNIPYLGEIPITPALREGGDKGVPILIQDPKSEVS
                     RIFLEIAAKLAGQLSIASERAKQTQGLKIVSS"
     gene            23340..23639
                     /locus_tag="VF182_10420"
     CDS             23340..23639
                     /locus_tag="VF182_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018031534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEX6437535.1"
                     /translation="MITKEQVYEALHECYDPEIPVNIVDLGLVYDVQVNDDNVAVKMT
                     LTAPGCGMGTMIAADAKEKILALEGVSDATVDLVWDPPWDPSRISDEAKQRLGIG"
     gene            23727..24977
                     /gene="amrB"
                     /locus_tag="VF182_10425"
     CDS             23727..24977
                     /gene="amrB"
                     /locus_tag="VF182_10425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX6437536.1"
                     /translation="MNLFPRLRAVEAFPVQQNGNDLIYLKDPLNLATPLGVSPIGYFL
                     MAHFDGRHSFIDIQEIYSKQFGTVLLTDELKRFVDMLDEHYYLLSERFTEYQKSVIEA
                     FSLAPARAPAHMGGVYPNDAVALKAQLDGYFNSPEGPGLALTNGRRMTPQAIVAPHID
                     FHRGGSAYAWAYKPLLESDGADLYILLGTSHCGGNTPFILTLKDFETPLGIVETDKTF
                     VSHLQNACQQDLFADEYLHRGEHSVEFQVLFLKYLAQRRAELLGQPERPFKIVPILVS
                     SFHEMVATKTLPERTPTVGTFLGALKSLVDSERREVCFVAGVDLAHVGRQFGDGEPVT
                     EDFLKWVEAEDDRLVERLAARDAPGFFNEIAKDHDKRRICGFSPLYSLIHLLKKGKGQ
                     RLKYSQSFTPETASAVTFTSMIFD"
     gene            complement(25051..25809)
                     /locus_tag="VF182_10430"
     CDS             complement(25051..25809)
                     /locus_tag="VF182_10430"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX6437537.1"
                     /translation="MRLQDKVAIVTGGGVGIGKAYAHGLAKEGAKVVVADIQEAEAKK
                     VATDIKQSGDEAMAVAVDVTSFDKAQAMAQAALTAYGRIDILVNNAGLYSALKKKNFM
                     EIDSDEWDRVMAVNVKGLFHCVKAVYPAMKQQGKGKIINISSGTTLNGSPFFLHYVSS
                     KAGVIGFTRALAREVGGDNICVNSIMPGLTISGANQEGVMTAEQLADRRKRRSIQRDQ
                     YPQDLVGTVIFLASDDSDFMTGQSISVDGGMNMH"
     gene            complement(25836..26876)
                     /locus_tag="VF182_10435"
     CDS             complement(25836..26876)
                     /locus_tag="VF182_10435"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6437538.1"
                     /translation="MNVNYPIIDADGHVMETDAELREFLDGRYRQGPHFKTYSYFPSL
                     DGWNRGFGVPGKVPETPAPKWLEFLDELGVHTTVLYPTGGLSLGLIQNPEWSCVIARA
                     YNSWITERFTKQSSRLKAVALLPVHEPIEAAKELERAKGLGLVAGLLPAVTVLNKGYG
                     HTDFDPIYQAAEKLDMPLTVHGAPSRGMGFDFFNKFLHVHTLEHPFAILIQFTHMVFE
                     GVFSRFPQLRVAFLEAGSGWLPYMMDRMDEEMEKPYRFQAPLLKQEPSEIIRSGQVWA
                     TCEVEEQALPQVIKQFNPACLMWPSDFPHERLPDMFSHDLPEFLGRTDIGEEDKKAIL
                     HDNPARFYRLEI"
     gene            complement(27181..27951)
                     /locus_tag="VF182_10440"
     CDS             complement(27181..27951)
                     /locus_tag="VF182_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6437539.1"
                     /translation="MVEGLKDKVVIVTGGGHGIGKAYCLGFGQAGSRVVVADIDKPAA
                     DQVSAEVMQQNGAQALALHTDVADEKSTKEMAAAVLQRFGRIDVLINNAAIFATIPMN
                     RGRIETIDPAEWDRLMAVNLKGLFFCCRAVLPSMRAQKSGKIINIASGTVFAGSAGRI
                     HYVTSKMATIGFTRTLAREVGDDNINVNVLAPGNTLSEENPSQEVIRLRESSAGLRAL
                     KRIQMPQDVVGAMLFLASPLSDFMTGQTVNVDGGISFV"
     gene            complement(27978..29072)
                     /locus_tag="VF182_10445"
     CDS             complement(27978..29072)
                     /locus_tag="VF182_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX6437540.1"
                     /translation="MATVRGATLLGPDKLEIREYPMPDIPADGGLVKVEMGGVCGTDV
                     KYLHGKLKVDYPIILGHEILGRVEKLGKNAAAIHHVKEGDRIILKGALGCGRCSDCRR
                     NNQRFCRQRTNYGGRTKCDQPPHLFGGFADYVYLAPDVLATKVSDALPAEAAALIGSV
                     MANGFQWAIRRGGVKMGDFVLIQGPGQQGLSCTFASRHAGAAKVFISGIGRDQPRLEL
                     AKKFGATRTINVEEEDVVEVVRKETNGEMCDVVVDVSGNPNAILKSVDCLHRQSTMVL
                     GGLTGDTTVTPMLMDKLVWNEIKLQGCFTADNDATEAALRLIEETKFPVQEMVSHTFP
                     LNEIEKCIRAVGGEIPEMFPTKALIDPALG"
     gene            complement(29126..30112)
                     /locus_tag="VF182_10450"
     CDS             complement(29126..30112)
                     /locus_tag="VF182_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437541.1"
                     /translation="MKYGRHFLAGSLIVLGALCQGAVARAANINVVWTSEVGQFAPLW
                     ITKEAKLFEKYGNQVQLIFIQGAASAAAALSSDDAQVGMFSPQVVISTPALELVMFGR
                     LGNTMDNRIYARKGIKSLKEVKRIAISRFGSNADFAARYLLQREGLRPDTDVALLQVG
                     NQANRIAAVETANADAAMLTPPMTLQARKLGFSLIADASKLGIPYSSLFFVSRRPYLM
                     KNRADLVNFTKAMIEGVHYYKANKEFSMKVIGKYMKVQDREVIEENFREYDFPLKPYP
                     AKEYYELPIQEVGKKDPKVLKEDPERFADQSLVKELDASGFIDKLTKEYKVK"
     gene            30208..31185
                     /locus_tag="VF182_10455"
     CDS             30208..31185
                     /locus_tag="VF182_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437542.1"
                     /translation="MSRLLFAFIFVLLSTSDVTAQGLTRVMSGYAATSGPHAVLWLAR
                     EAGLFEKNGLRTDVAYIRSGSTMGQALVAGEIQLAQMGGPAALAAGVAGFDITMVAVA
                     LNTTPIVIMGKVSKMEELKGKAIGVTRYGSNTDISARFAIRKAGMQPEKDVALIQLED
                     YAGIMGGIQSGRIAAGALADPFTDHAKRLGYNEIADIAALGLEFPFVGIVTKKTFIKE
                     QPDVVQRFVRAYTESIALYKSNRELAKKVTSKYTGIKDPEILNSTVDFYAPKLASVPY
                     PTVGGIRFVLDQVATRDPRAKNFNPETFMDVRFVKQLEDSGFIQGLKRK"
BASE COUNT         7007 a         8381 c         8515 g         7365 t
ORIGIN      
        1 ccagtcctcg ccgtggccga cactggtcag acgttttttc actgtggttg tgcgcgccgc
       61 gattgctttg cgccagcgca gtccggcttc gatcagcgta ttcttgtcgt gcaccctggg
      121 ccgcttggtg ccccatgcga cgatggcctt aagttttttc gagcccatca cagcgcccaa
      181 gccatgggcg gcgttatgat tgtagtcatt gacgaacata gcggcacgaa tcagactttc
      241 gccggccggt ccgatacacc ccacccgcgc gtcctgatga ccggattcgt ctcggagcat
      301 gtcttcggtg tcgcgcgagc ctttacccca cacgcgccca gcatcgcgca attcaacctt
      361 gtcttcgccg atataaagat aagtcggctg ctgcgcagcg ccggtgatga tgacaccgtc
      421 atagccggcg gctttgagtg atgtgccgaa atagccgctg gttgcgccac ggcccaaggt
      481 gtagcgagtt gccggactga gatagacgaa gcacattttc gtgccgcccg gggtaaagcc
      541 ggttcccgta atcggtccgg tcatgccaac gatgacgttg cgggcatcat agggatcgat
      601 atttatcggc aattcgttaa gcagaacgta ctcagcgaac agttgaccgc cccagtattt
      661 tcgcaatatc ggctcttccg gaagctgcaa gtcgaccagt ttttccgtgc tgagatcgac
      721 gcgcaagatc ttacccatgt aaccgccggg gaggcgcgtc cggcgttggc cggggaagaa
      781 gtttctatag tcggagcgat cagtcattga cgttcactcg ggtttatggg tccgaattgt
      841 ttaaaccgtt caagccatta taccaatcgg cgcgagcctc ggtgcagcac ttgaatgttt
      901 tgaaccgctg ccactgaagc agtccgtgta tatcagtccg ccgccgccca aagcacccgg
      961 tcggccggca agtcgatgtc gagtctccgc caaccctcgc ccgcatcgcg gctgatcatg
     1021 aggtcgccgg cgtttcctga tccactggca tggcgcgggc aacgttgcca aatccggcaa
     1081 aaagcgcgcg gtggtcggtc gggtgatgca ccagagccca gatcatcgag tcgagatcgt
     1141 cggcgatacc gtcggtaatg cgcgtccatg atccggcgcc gtccgcgctt ttgaagaggg
     1201 cggcgcgtgc gccgcgattc tctctgcgcc agaagccagg cgactggtct gcggttgcca
     1261 aaatcatcgt cggcttttct ccgcttataa ccggcggcaa aaacaggaag tcgtgaaagt
     1321 aattcctggt cagaccgttc tcggcgcgca gccaaccgtc ctcgggtttg tcactggtga
     1381 aaaagccgcg agcggtggcg acgtaatatc gatccattcg cttcggtaga ttggcgatca
     1441 cgtggatgtc cagatagtcg atgcctttgc tgacgtcctc ccagctcttg ccatgatcga
     1501 agctgcggac aatgccgcca tgttcgatgc atagatagat tgtgctcgga ttatcgggat
     1561 ggatgaagat attgcgaatg tggcccttat gcggcggctg aggaaaccac cagttctttc
     1621 gtgccgtctc cggcacgtct tgcagcgtgg tcaattcctg ccagtgattg ccgccgtctt
     1681 cactccgata aagcgccacg ggttcgacgc cggaatagat caccttgtca tccgttgggt
     1741 ccaccgcaac ggaacgaatg tcgccatcca aaactttagc ccaactctcg ccgccatccg
     1801 tggtgcgata cagcccatca tgcgtaacgc cgacgaagac ccgctcgggc atggtcttac
     1861 agccgaaaac ggaatcgatg atgccatcct cgaaatggcg accgacctct tgccaaccgc
     1921 gtgcgaccga tcgccaaatt atcagtccgg cctgcgttcc cgcatagaga cgaactcctt
     1981 cgttcatcgg acctcccctc gttcaccgct ggtatatact gaatttttac gactaaaccc
     2041 ccggttttgt tcaaaccgtt caagcagttc aaagtgttca aatgatctcg gaaaaacatg
     2101 gaggtgctgt ttgaacgggt tgggcgattt gaacgagttc agctgtcttt ctccgcttat
     2161 ggaaacaact ccatcctccc ggttccggct tgccgatccc aggtggattc caagcgtgtg
     2221 aaaaagtccc atgcacgctg ggaacggcgt tcgaagtcga tttcggcatt aaagccacgg
     2281 cattcttctg cgctgaaatg atcgacagga ccgagcgtca aagcctgcat ggtctttttg
     2341 gcatcgtctt ctaaaatcat cgatgcaaac aaaacctccg cgagactttt cccggcgacc
     2401 acgatgccat ggccgcgcat aagcacggcg cgatgattgc ctatggtctt caacagattg
     2461 tcgccgcgtt ggggcgtcgt cactagatga gcttctttgt aaagtggcac gccgtcaccg
     2521 aagatcgttc cttgcaaggt gaccgggtga aactcgcgcc gggctatggt gaacaaggtc
     2581 gcataagcag tatggagatg agccactgcc aacgcgtccg cacgggcacc atgtagagcc
     2641 gtatggatcg gccactcgac aggaggcaga gtcttgccgt ccaatggctt accatccagg
     2701 tggatcggga tcatatcgct cgcctgaaaa cgtggcttgt ccgatcctga tccgggtgtg
     2761 acaaagatcg tcttggtccc gggctgataa acgctgacgt ggccgaacag gccgattagc
     2821 ccctgcatcg cgaaaatccg agtacaggtg gcaagctgct ctcgcaagtc tttctccggt
     2881 gtcatcgact aggtctcctt tgtgcggctt ctaattcctt cttcacttct tcaacgagct
     2941 gcaaatgtaa caaacggtcc ggaggaatat cttctctgat ctgcagcggg atcttgatgt
     3001 catattcaag aaagtttctt atcccttcag ctgtcggaag tcccgggacg ccaaccggta
     3061 aggcgcgcgc atagttttta gttccttcaa caatcatcgg gcgtgtcgct ttgcctaatt
     3121 gaagcttttt aatgccgagg ttgaccgcct cctcaggttg gtcgcgcacg aacataagcc
     3181 cgcgaatcgc tgccttgagc cagcgcttga tctgccgtgg gttttcgcga atttttctat
     3241 cgagagtaac aaatccctga aagggcatgg gaaaaagatc accgagaaag cccaacgaaa
     3301 ccaatccctc tttttgcgca aacacaaggc cacccggatc caccgcgctg gcgtgcgccg
     3361 cgcctgactt gagcgcgaga attcggctgt tggtgtccga acccatggcg atgcgggtaa
     3421 gatctctgct ggaaacgccg ccgcgttccg caaacatagt aatcattcgg tcctgcgtat
     3481 cgttcgaact gccgaccgca acctttttgc cctttagatc tgcggccgtc ttaatgcttg
     3541 gatctgcaat caagtaaaac gtaacttttt ctgtttgaaa aaaaatgccc ttgaccggcg
     3601 cgcctttgag cgccgcccgc atgccgctgc cgccgcctcc gctaaaatcg acatcgccgc
     3661 tcaagagcgc cgcagtggca agcggtcccg agatcacgac cgtctcgttc tcaagtccct
     3721 catcgccgaa gaagcccttg gcgtcagcta cgtaaatgga aagatagtta agattggctt
     3781 gcggaatggc ggtccttatt ttctcggcgc aaacttcagt cgtaaatgag accattaaga
     3841 gagcgctgag gatccatggt cgtgcaatga acgaagaccg aaatttggac ggtctcatgt
     3901 ttgataatcc ccaggttaac ggttcatgaa ttaagtggct ggggcgggca ttagttgtcg
     3961 taacgctcac gagaaataac gatagattag ccgcttcgga tgaatgatgg ccaaatagaa
     4021 tgcgcgcaac ttgcccttga taccgcaacg cggcgtaaac agatccctca gcatcaagcc
     4081 gatcggtatc ttgtgctttg gaaatttttc ctcgccgata ctgcgcaccc catatttttc
     4141 cttattttct ttgagcacat aaattacgtt gccgccgccg gcttctttgt cgccgtaggt
     4201 gtaaccgcca acggttcccg cttgcttcct tgctttcgcg gcaatctcct cggttaaacc
     4261 gaaatccaat gcctgggtag gacaagcttg gacgcaaaac ggttgcttgt cttcgctgat
     4321 acgatcactg cacatggtgc atttgctggc tttttgcgtg accttatcct ttgtgatgac
     4381 ttcccaaggg caagcgtgaa tgcagtattc gcaaccgatg cagatttcct gattgatgac
     4441 taccgggcct tcttcatact tggtgatcgc ttccaccggg caaaccgccg cgcaggcggc
     4501 gaactcgcaa tgcatacaac tgttgcgtgc ccaaaggggc gagagcccct cgagcacagg
     4561 gggttccatg gttatccagt tggtgctgga gagagaatag aggttggtgc ggtcgtgatc
     4621 gttccagtcc ttacaagcct ggacacagtg gcggcagccg atgcacttgg tcgcatcgaa
     4681 cagaatactt cttgcattgc catcatggag cctcccggtc ggaaccaggt tgccatactg
     4741 ttgaatgctc tcgcccgggc cagtcagact atcgcgcatg ccccggatgt atcacttctt
     4801 tttcgcagcc gtcaactatt gccaggcata cggagagcca aaactagtaa aaacttagaa
     4861 ttgaaacgta aacaggtggg agtaaaaggc ggctattgaa gacgctcagg gagtaagcga
     4921 tccaattcgg cctgccggag cgcggccttg cgcccgctct cgacaagccg aatcgatgat
     4981 gtgactcaaa gaggaattta caagtcgaac gatctaaaca tcatcacggc ggcgaaccgt
     5041 gcccgaggca tcgcgtggca gcggtttgtc ggtttccgcg aaatcagcgc tggcctcgct
     5101 ggtcgaggac acatcctggg ctccggttct ctccagtatg tccttggcgc ggctcgtcca
     5161 ttcagaattg tcgcaatgga ctgagaccag tatcccgccg tccttgattc tgccctcgta
     5221 tctcttggct tcatattcgg gaatacccgc tccgaccaaa gcgccggtca agccgcccag
     5281 cgcgccaccg accccaacgc cggcgagtgc agccatgatt ggccccgccg cgataaaggg
     5341 tccaacacca ggaatcgcga tcgcgccgat accggctaac caccccaaca cgccgccagc
     5401 tacggctcct gtacccgcgc cacttgccgc tccctcagga gctttggtac ccttcctgtg
     5461 cgcaaagtct ttggtgcctt cgttctctgg aaataagacc gagatgtctt cgttgcgata
     5521 gcccgcctgt tgcagagcgc taattccatc ctccaaagcc gtttcgttcg gatatattcc
     5581 aaatgctgca gtatttttac ctgccatatt atggtactcc tttcgatcgt cgttgaggca
     5641 cagtagagcg aatcacgtgc caatcgatcg tcccgaaacg gtgcagacgg gagtgaactt
     5701 tttcgaagtg atgtcccaaa ccgtggaaac cagcaggtgg ggcaagcccg catctgggac
     5761 agcattttga agataactgt tgaggctgct tgagggcagc cgaacgctaa gcctcgttgc
     5821 ttcctattct atctctgatt atgatctggc atctatggcc cagacctttt tcaaagtgtt
     5881 agcgccgcaa gaagcgttac gcttgcttgt gcaggttaat cccgtaccca ccgagcgtgt
     5941 tgcgactgtg accgctcgcg cccgggtgct ctctgaggat cttcgttccg ctgttgactt
     6001 gccccatttt aatcgcgccg caatggatgg ctatgctgtt cgttccaaag ataccttcgg
     6061 cgcgagtcaa agcctgccag cttatttaaa acttgctggg gttgtcgaaa tggggaaaga
     6121 ggcgatccag acggtgcaaa cgggcgaagc ccttcgcatt tccaccggcg gtatgatgcc
     6181 gccggcaagc gatgccgtag tcatggtcga gtacaccaat gaagttgaag gcgggctggt
     6241 ggaagttcat cgcggtgtgt cgccgtggca gaatgtcatt caaatcggcg atgacatcaa
     6301 aaagggcgag ccggtttttc agtccggcag gagactcaag gcgcatgata taggcgccct
     6361 aacaggcatc gggctttcat cagtgccggt gttcaagcga ccgcgggtgg cattaatttc
     6421 caccggtgac gagatcgtcg atgccgacgt cactcccttg cccggacagg tgcgcaatat
     6481 caaccagcac tcgctggctg gtttgatcgc cgagtgcggc gccgaattga gagacttcgg
     6541 agtcataccc gatgatcgcg agcggctcac gagcgcgata acgcaagctc tagattgggc
     6601 cgacctcgtg ctgttgtctg gcggcagttc tatgggcgcc aaagatattg cccttgaaac
     6661 gattctttcg tttccggatt cggagtttat atttcacgga atatcggtag cgccgggtaa
     6721 gccgacgatt tttgccaaag catgcggtaa gccgatcatg ggacttcccg gatatccggt
     6781 ttccgctctg gtgatctttg atcttttcgc cgcgcctctg atccgcagac tgggcggtga
     6841 ggtgattgcg acacacgccc gctttgctag gacggccaaa gcaaccttga agaccaacat
     6901 cgcttcccaa atcggccgtg aagattacgt tagggtagct ctcgagcaag aaacacaggg
     6961 gcttctagcg actccgttac caagcaaatc cggcgcgatt tttacgctcg tgaaagccga
     7021 tggcatggtg cggatcgatc tcaatcaaga tggtttggaa gccggagaag aggtcgaggt
     7081 cattttgttc tagcttttgc tgatcgcgcc gttggtgacg gtggctccac cccagtatgc
     7141 ctcatagccg agaagaaatt ctcgcacccg ataaaagatc ggtccccagg agagttatgg
     7201 ctcgcaaacg ttatcttaaa aaaactccgt tgaaagaagc caaagaattt tttctagcca
     7261 aagtcgagcc gtcacggatt ggcaatgaat ttatttcggt ggaagaggct ctcgaccgca
     7321 tcaccgcgga gccggtcttc gccaagatct cctcgccgca ttaccatgct gccgccatgg
     7381 atggcatctg cgtgcgcgcc gaagatactt ttggcgcaac tgaatttaaa cccaaaacac
     7441 taatctgtgc gagcagcgac tcgcccggtc cgaacgcgtt tcattacgtc gataccggca
     7501 actcgctacc ggcatgggcg aatgcggtga tcatgatcga aaaggttcgc cagctcaaag
     7561 atggatcgtt agagattttt gaatccgtcg gaccctggaa tcatgtccgt ctggtgggcg
     7621 aggatgtcgt tgctacggaa ttgctcttgc cgcgctatca ccgtcttcgt ccctatgatc
     7681 tcggtgcttt gctggcctcc gggcataccc tggtagaggt gaaatcccgg cccaaggtcg
     7741 ggatcattcc aaccggtgat gagttgatcg cgcccggcga gcaacccctg ccaggcgctg
     7801 ttatagagtt caattcaacc attcttgcgg cgtttctgcg cgagtggggt gcagcgccaa
     7861 tcaaatatcc gcgtgttaag gaccaggccg tgctgctcga agaagttgtg cgccgcgccg
     7921 ccgcggaatg tgatctggtg acgatcatcg ccggctcctc ggcgggagaa catgatttga
     7981 cggcggacgt agtggctgat ttgggtgaac tctgggcgca tggaatcgat gtcatgccgg
     8041 gcaaaccggc ggtgctggca ggagtcgccg acaagccggt gatcggtatt cccggctacc
     8101 ccgtctctgc tatcgtgatc gcacgggaaa tcctgcggcc cgcagtggaa aaatttctgg
     8161 gcgccgcggc gccatcctat tcgatagttc atgctgtggt gccgaagaaa atcgcttcgc
     8221 acctcggcct ggaagagttt atccgggtat cggtgggacg ggtggagaac aaattggtgg
     8281 cggtcccgct tgcgagaggc gcaggggtga ttacaaccat ggttcgcgcc gatggattgt
     8341 tacggattcc aagtctcgtc gaaggattaa acggaggtga agaagttgag attgagttac
     8401 ttcggcctcg agcagatatc gaaaacacga ttctctgcac cggcagtcac gatctcgcca
     8461 tcggcgtgat cgaagatcaa ctcaagcagc tttacccgga gcttaagatc gccgctgcca
     8521 atgtcggtag cgttggcggt ttgctggcgc tgcagcgtgg tgaaacccac atggccggca
     8581 ctcatctcct cgaccccaac accggcgttt acaatgttcc ggatatcaag cgaacaattc
     8641 ctaaattgcc ggtggcgctt gtgcacctcg cgcggcgcga acaagggatt gtcgtcgcgc
     8701 ggggcaatcc taaatcgatc ggcaatttaa gcgatctcac gtccggaatt cgatttgtca
     8761 accggcaatc gggatcgggg actcgcgtgc tgctggattt tgagctcaag agactgggca
     8821 tcaatcccaa ctcaatcaac ggctatgatc gggaagagtt cacccatatg tctgtggggg
     8881 ttgcagtcgc cagtgattta gcggacgcgg gtcttgcggt gcgggccgct gctcaagcct
     8941 tggggcttga tttcataccg atcgccagtg aacagtacga cttgctgttt tcgcgctcat
     9001 tcttcgaatc agaccgcggc gagcggctga tgctggtgct tcaatcgaga gcgttcaaag
     9061 atgctgtgag cgcgttgggc ggatatgaca gtagcggttc cgggaagctg ctctaccagc
     9121 agtaggcatc gtattcgtaa ctgtccgcac tctcgagatt gatggtggac gggtaaagtt
     9181 cgagatccgc acggtgttca ctcacacgca ttgagatcgt tgtctggcaa ctcgttcgct
     9241 cttaaagatc gtctctctca agaacctctt cactgacgac tcctgcgtcg cgtcgtcgca
     9301 gattgtccag atttgtccac ccacttcgcc agagtcttac ttttggccct aaatcacttg
     9361 ctttaatcag tacttagact ggtagtgcca tggcattgcc attgcgttag cagccgccgg
     9421 gagtttcttg agtcctatgg atgaaaagat cgacagcatc cgtttactga cccttgacga
     9481 ggctgcggca ctgctccagg tttctaagcg cactctccac cgaatgatca agaccaatga
     9541 gctgccggcc tttaaggtag gcggccagtg gcgggtacga gagactcaac ttaggcaatg
     9601 ggttgaacat cgagaagccg gcagcacgga gcgagagagg aaaggcaatt tgtaagaaaa
     9661 gagttaatct ttcatcggga cctttcgaag tttttactcg gacattcatt tccgctttca
     9721 aataggctcg ccggaataaa ctcagttgca gacacatcga catgcccaaa actaagagct
     9781 tggctaagcc tagaagcttt gcgcgagcga catcttccgg gttcctgcag agcttttggg
     9841 agttcctgcc ttccggagaa tcattaccgc cggccgtctg gcaaagccgt catctatttc
     9901 tattgcggct gacgtggctt cacgcgttgc tgctcctgtt gatcgcggtc ctgtatggtg
     9961 aaagctggga atttagcctg ccagccgtgg ttcgcaacgg cacggtgctt cagacggtcg
    10021 gtaacgcggt tatagtcggc cttttcgcaa gtgttgccgc tctaacctcc gggcgcgctc
    10081 ttccggctgc agcagtcggc ttcgggcttc ttagcgcttc ggcttttctc gttcatctct
    10141 ccggtggtgc catcgagttt cactttcatt tttttgttgt gcttgccttc ttggcgcttt
    10201 accaggatcg agtttcgtac gttctggttc tggtttacct cgtgatttac cactttgttg
    10261 tcggtgcaat atggcctcaa cacgttttca accatacggc ggctctagcc gctccctgga
    10321 gttgggccgc gattcattca ttctttttga tcgccgccgg tatcggcaac atgtctgctt
    10381 ggcgcttcaa tcaaaaggct ctcgcacaga ttaatttaat cctcaattct gcgaccgaag
    10441 gtatttatgg cattgacctg gagcaaagaa ttctctttat gaactcggcc ggcgctgaac
    10501 ttctgcggtg tcggcctgat caagcgattg gaaaaacact gcagcaagtg ttgcgacaca
    10561 ctcgctctga tgggacagca ttttccaacc agccgtcgtc cattgttgct tcattaaggg
    10621 acggcgccaa gcaccaggcc agcgatgagc tattctggcg cagcgacggc tccagctttc
    10681 ctgtggaata taaatgcgct cctattttcg accgaaacgc gctggcggga acgattgtta
    10741 cgtttagcga tatcagcgcg caacagcaaa gagagacggc gttgcggcag agcgaagaac
    10801 ggtttcgcca gattgccgaa aatatcaaag cggttttttg gattacgaat ccgatgaaca
    10861 acgagaagct ttacgtcagc cccgcatacc gcgaaatctg gggacgcgat actgccaaga
    10921 tagggtcttt gtcccaatcc tggctggggc tactccatga agatgatcac tcacaagtgc
    10981 tcgaggcggt gggaaccaag caggtaaccg gtcagtacaa cgaggagtat cggatcgtac
    11041 gcgaggatgg ctcaattcgt tggatcaatg atcggggctt tcctgtgcgg aacgagtccg
    11101 aagaagttta tcgcatcgtc gggttagcgg aagatatcac ggcacgtaag caggcacagc
    11161 aagatttgct agaccggtac accgagatga acgttttgca caatgtcggt caagccgttt
    11221 tgagcgctac agacattgag cttcttttgg aaaacattct cgagcaagcg ctcgccactc
    11281 tctcgctcga gatcggtaat attcgcctgt ttgagcctat cagcggtacg cgcatcggaa
    11341 tttatcgagg ctacggcaat caagaaaata cccgcgttta cgccgacatg aagaatccca
    11401 gaagcgatgc tctttctatg cggatcatcg agtcaggaaa aagtctggta ttcgaaaatc
    11461 tcgcagcacg ggacggattg cctgcattta caaaggaaca ggtgtgttcg gctcttgtcg
    11521 tgccgatcat cctggaacga gagacgctcg gattgatcga ggtgggaagc cgcaagcgct
    11581 ggacgtttcg gccccaagac atgcgcctgt tgaatgcgat cggcaaccaa gtaggtctcg
    11641 ccattcagaa ggcacggctt ctccatgaga cggagcgccg cgccaatgaa caggcagcgc
    11701 tcaatattat tgccaaagct atcagccagt cgcttcgtat cgatgaactg ctgaagattg
    11761 cgttggaaaa gatcttcgaa gtcaccggac gcgagcgcct attaataagg ctcaaagaat
    11821 ccgcttctaa cggaatgata ttggcggcgc accggggatt tttgccggag gaaatcgaag
    11881 agttcgctaa agcgtcatcc gacgccatca ccggggaggt gtttgcaagc gggcagccgt
    11941 tagtcatcaa caattctccc gctatcggga gtcctaaagc aatctcaccg gaaaatccgg
    12001 ccggcatgat cccgataaaa gccggcactc aggttatggg gatcttgagg gtctctgcgg
    12061 tccgaccgat tccctttgaa ctgcgcgaac tggaattgct agaggctatc ggaaacatgc
    12121 tcggcgtggc gctcgaaaat gcgcgactgt tcagtgaaac cgaggcacgc taccgcgaac
    12181 tcaagacgct ccaggctatt agtgacacta ttctcgagac tcttgattta acgatcatga
    12241 tggagcgaat tcttgacagg gcatttgaaa tcggcaggtt tgattgcggc gtgatccgcc
    12301 tgttggaccg agatcatgaa agtctaaaac tagtggccag ccgtggatat cgcgatcgtg
    12361 aaggagttct cgaccattac gaagccagtg agcaaaaatc caccgcaaga attatcgcgg
    12421 aagtaatggc gagcaagcag accaacgtaa agaacctgag tacatcggac ggcatgcgca
    12481 cattcagacg agaaggagtg tgcacggtgg tgactattcc actccatacg cagcaggacg
    12541 tgctcggtat cattcagctc ggcagccgga tcacgagaga gttccaggac agtgagttac
    12601 gaattttcga cgctatcgga ggtcaggctg gcatcgccat tcaaaaggct cggctctatg
    12661 atgaaagcca acgggcacag gcggcattga acgaaaaggc ggcggagttg acgcgctcga
    12721 atacagatct acaccattca acccaggagg ttaaatcggc gaaagagaaa ctagagcgcg
    12781 taaacagtct gctgacggtt caggccgccg aactggctcg ctccaatacc gaattggagc
    12841 aattcgccta tgtcgcatcg cacgatttac aagagccgtt aagaatggtt gcgagctatg
    12901 tgcagctgct tgcccggcgc tacaaaggca aactcgatag cgaggccgaa gaattcatcg
    12961 gctttgcggt ggatggctcc aagcgaatgc aggacttgat ccaggcgttg ctggcttact
    13021 cgcgcatagg aaccaagggt cgccagttcg cgccgactga ttgcgagatc gtgcttcaaa
    13081 acgctctcaa gaatctgcag atcgctatcg aagacagtcg agcgagaata actcacgacc
    13141 ctctccccac cgtaatgggc gatgctattc aactaggcca gctattccag aatctaatcg
    13201 ggaatgcgat taaatttcgc ggtgaaaagc tcccttccgt tcatgtgacc gcggagcgtc
    13261 acgatcgcga gtggctgttc tcgtttcgcg atgacggcat tggcatcgac tctcaatacg
    13321 ccgaacggat cttcgttatt tttcaacgtt tgcatactaa ggaggaatat ccagggacgg
    13381 gaatcggcct tgcgctgtgc aaaaaaatcg ttgaacgaca cagcgggcga atctgggtgg
    13441 agtcagagcc gcacaagggc tcgatctttc gctttacgtt gccggcggaa aatcccgttg
    13501 aaggagataa acccttatga atgtttacaa cggctgcatt caggtgctgt tggtagaaga
    13561 caatcccggc gatgtccgcc tgaccaaaga agcgctcaag gaaggcaaac tgctaaacca
    13621 gttgacggtc gtcggcgacg gagtagaggc actctcattt ctgcgcaagg agggggtata
    13681 cgccgatgcg cctcaaccgg aattgattct cctggatttg aatttgccga aaaaagacgg
    13741 cagggaggtt ctcgctgaaa ttaaagccga tcccaacctc cggcggattc ctgtggttgt
    13801 gctcacaact tcttcgtcgg aagaggacat actgaaaatc tacgacttgc acgccaactg
    13861 ctatatcacc aaaccggtgg acctcgagca atttatggga gtggtgaagt ccatcgaaga
    13921 tttctgggtg agtgtcgtca agttgccttc gtacgaggcg actaagtgag cagcgcgccg
    13981 attcaggttc tgttgatcga agacaatccc ggcgatgccc gccttctgcg cgagcttttg
    14041 caggagacgg gaagctccca attcgaactg gttcacgcgg accgttttag cgaagcctta
    14101 gcgtggctaa gcgaacggcg atttgacgtg atcttattgg atctgtcgct acccgacgcg
    14161 cagggtctcg ataccatcgg ccgcttgggc gcgcaggccg aaggcacgcc aatcgtgatt
    14221 ttgaccggtc tcaatgatga agagcttgcc atccgggccc tccaacaggg agcgcaagac
    14281 tatctggtta aaggccaggc cgatggtcaa ttgttggcgc gcgcgctgcg ttactcgatc
    14341 gagcggcata aagccgaaga aagtctgaaa gcacgaaacc gagaactgat ggtgctgagg
    14401 aaaatcagcg aggctatttt ggcatccttg gacctcaaca cggtgctcga acatattctc
    14461 gagcaggcaa tttttattgg ttcgttcgac cttggcaata tccgattgct cgaatccgac
    14521 ggcgatacgc ttcaggtggt ggttgttcgt ggttaccgag atctggggaa cgtcgcaagc
    14581 caccgcgcaa tttcccgtaa caccggcatc gcccaatcct ctcatttcaa agagttagta
    14641 ttccagcagc cgtgcgtcga ggaagctgtg caaagctgcg atggcctgaa aactcttaaa
    14701 aaggagggag tgcagtcgtt tgtccaggtg ccggttcgcg ccgatggtga ggttttgggc
    14761 attattcaac tggcaacgcg gacgccgcgc aaattcaagg ttgaggaagt gagtctttgg
    14821 gaaacgatcg gcaatcatgt gggagttgcg gtgcagcgtg cgcagcttta tgaggaaacc
    14881 aagcggcaag cgcgcgagct cgaaaaggca agcaagctgc aagctgactt ctcggcaatg
    14941 atcgcccatg atcttaggtc cccgctcatg aacataaccg gagttacaga ggtgatgctg
    15001 gatgggatgt tcggcccggt gaccgaggag caaaaaaaat ggttgttgcg ggttcaggcc
    15061 aacagccata atctaatcga cttagtgagc gactttctcg atttttccaa actcgagtcc
    15121 ggctacgtca atgtcagcaa acaagtcgtc agcgtcacgg acttggttcg gaagagcatt
    15181 gagagttttc aggtggtagc acagaacaaa caagtgacgg ttgccgaggc gattgatgcg
    15241 tcgctgccat cgatccttgc cgatccgcag cgcttggagc aagtcttaag taatctgatc
    15301 agcaacgcca ttaaatttac cggcgatggc ggcgaggtaa aggtcggagc agctttgact
    15361 gcgacaggcg gtattcgagt ctgggttcag gatagcggcg aaggcattcc ccgagaggag
    15421 ataggcgagc ttttccaaaa atatcgccaa ggggggaatg ccaacagttc gaatcacaaa
    15481 ggcaccggtc tcggtctggt gatctgtaaa atgatcgtag aagcgcacgg cggcacaatt
    15541 tgggccgaca gccaggaggg ccaagggagc atcttctcgt tctcgctgcc ggttgcagcg
    15601 taacccatcg gggtgaattg gttcatagtc acaccggcat taggccatgt ttcttcttcg
    15661 gtcgctcttc tttcttgttg cgcagtatct ccaaagatcg gatcagccgc tggcgggtct
    15721 gtgctgggtg tacggctgcg tgcgcaaatt gtttggacag cgcttcaaag gggccgctaa
    15781 agcgctcctg gaactcggcg attttttctc tacgagtctg ctccggatcc gctgatcgtt
    15841 cgatttctcg cttgtaaaga acgttgaccg ccccgccgcc ccccatcacg gcgagttcga
    15901 ccccgggcca aacgaacaac tgatcggcgc ccatttcgcg agtacagaga gcctgtttgg
    15961 cgccgccata acctttccgc aagacaacag ttattttcgg caccgtcgcc tcggcatacg
    16021 cataaagcat cttagcgccg tgacgaatga tgcctttttc ttcttgttgt cttccaggga
    16081 aaaatcccgg tacgtccatc aatgtgatga tcggaagatt gaaagcatcg cagaagcgaa
    16141 tgaagcgagc ggctttgtcg gatgcgtcga cgtccaaact gccggcgaga aacattggct
    16201 gattagcgac gaatccgacc ggatacccat ccaacctgcc gaagcccgtc accaaattgc
    16261 gcgcgaattt cggtttcaat tcgagaaaat cattatcatc gacgatcgct ttgatgactt
    16321 gaagaatatt gtaggcgcgc cgcatatcat ccgggacgat tttgttgagc gcatcgagac
    16381 tgcgatccgg atggtcgctg gtcgatttgc gcggcggcag agattcaaaa ttgggtggca
    16441 agaaaccgag cagccggcga atcgtcgtga gacattcttg atcgctcttg accaccaggt
    16501 ccgcgacgcc tgagatttcg gagtgaattt tggcgccacc gagctcctcc atagtgacgg
    16561 tttcgccgag tacttctttg atcacggccg gacccgtgat gaacatctgg ctggaattct
    16621 gaaccatcaa aacgaaatcc gtaagcgccg gcgaatagga gccgacgccg atacacggcc
    16681 ccatgattgc cgagatctgg gggatgcagc ccgaggcgat actgttctcg aagaaaatct
    16741 tgccgatggc ggaagtgaca gacagcccct cctgaattcg agctcccgcg gagtcattca
    16801 gaccgatcag aggaacgccc aacctgcgcg ccgtctggat cacataggcg atcttttctg
    16861 cgtgggcact gcccacgctc ccggccatga tggaccgatc ctgtgaaaaa acataaaccg
    16921 ggcgcccgtc gactttaccg aatcccgtga caacgccgtc ggacggctgt tttcgttccg
    16981 ccatgccgaa atccgtgcat tgcgtttccg ccagcataaa ctcttcgacg aacgtgccgg
    17041 gatcaagcag cgtttcgact cgttctcgcg cggtgagcgt ggcttgatcg ggttgtttgc
    17101 cggtcgcctt tacagtgccg gcgaccgcgg ctttttctaa cgcgctaagc tttttttcaa
    17161 gatcgtctct cgatttggcc ataacgtcct gtcatccgcc gacgattccc attccagtta
    17221 acacaataag gcttttctca agatcgtctc tggatattta catgactttc cgttatcccc
    17281 cgacgattcc cattcccgtg agcaccgaca gcatcaccgc agccgcaatg acggaaccga
    17341 tctgcccacc ggcattggcg ctcatcgcat gcatcaagag ataattcttt ttattgtact
    17401 tctgaccttc cgtttgcgcg acccgggccg ccattggaaa tgctgaaatc cccgccgcgc
    17461 cgatcagagg attgatcttg cctccggtaa gcaggcacat gagtttgccg aagagcaccc
    17521 cgacgacggt gtccaggcta atggcgacca gcccaagtcc taagaccagc agtgtttcgc
    17581 cgcgcagaaa cttatccgct tccattgtgg cgccaatcgt aaggcccaaa agtaaagtga
    17641 tgatattggc aatctcattt tcggaagcag tttttagccg ctccacgaca ccgctttctt
    17701 tcatgagatt gcctaacatg acagtgccga tcaaaggtgt gccaatgggc gcgaggatcg
    17761 aagcgaagat tgttacaacg accggaaaga gaatcttcgt ggtcttcgaa accggctgcc
    17821 ggattacacc catgacaatt tttctttcgc gctcagtcgt cagcatgcgc atgatgggcg
    17881 gctggatcag cggaaccaaa gacatgtagg aataagcggc aactgagacc gcccccaata
    17941 gatgcggcgc atatttagaa ctgacataaa tcgcggtggg gccgtcgcat gcgccgatga
    18001 tagcaacagc tacggcgtcg agcttgtcga accccactgc caaagcaagg agtagagtca
    18061 gaaaaatccc gaactgtccg gcagcgccga gcagaataat cttcggattt tccaacagcg
    18121 gcgaaaaatc ggtcattgcg ccaataccca ggaaaatgag cacgggaaat atttcggtga
    18181 ggataccatt ctcgtagaag tagcgcagga tgccgtgctc atccatcagg ccggctaaag
    18241 ggatattcac tagaatggcg ccgaagccga tgggaagaag cagcagcggc tcataccctt
    18301 tgctgattcc aagataaagc agcagcgatc caatgaggat catgacggca tgacctaagg
    18361 tcagattgct gatcccaagt agaagacctt gaaggcctga agcaatcgcc gattccatca
    18421 gtttgtctag gctttcttcg gtgccgttgc ttcgggttta tatggaaaca tctttcgaag
    18481 gagcaggatc agcaggctta tgcaccacaa caccagcatc gttccagcca tgccaatgat
    18541 cgacagcgtc catccgaacg tccagatgtc catcagttta tctcctctca agattccggc
    18601 ggcgtggtga tcggcgagcg ggattatgcg atttctgcca gcagttgatc tgcttcgaca
    18661 gcctgtccga ctgacacctt gatctctttg agcactccag aggcaggcgc aacaatgggt
    18721 atttccatct tcatcgcttc catcacgagc actacttcgt cttcatcgac gcgatctccg
    18781 gcgttttttt caagctttaa gattttcccg accatcggcg ctgtaatcgg tgtcgccaag
    18841 ttgttctcct tgatccttga ggcacgtgga atactgccat ttactagagt tttaccgtgt
    18901 tgttgtcaat gctcgtagag gcgaatttga ctatttgcgc cccggtaaca aagccgcggc
    18961 ataccggtgc ggagagatca cgatcctgtt cctgccggac aaaaaaactc cgctgtaaaa
    19021 ccgcgtcaga tcggccatct ccgccttgct tttgcagatc tgatgcgata gaaaccgaga
    19081 tagttttggg gaggattcta tatggctaaa cctgcggttc aacaagatgt ttcgatcgat
    19141 aacattccgt attacgaaaa gtggcagctt ggggagggaa tccccatcgt caaaactttt
    19201 tttgtccagg acttaagaaa agttgaacta aaaccatggg accgagccgg cggcaacggc
    19261 gctttcatca acatggaagg cgcggaagga acgaccggcg cctatgtgtt ggaaattccc
    19321 gcgggaaaga gcctcaaccc gcagaggcat ctctatgaag acatgcttta tgttctcaag
    19381 ggccggggcg ccacgacgat ttggaatgac aagggcgcga agcaaacgtt cgaatggcaa
    19441 gagggcagcc ttttcgcact gccacttaac tcctggtacc agcacttcaa cgggcagggc
    19501 gacgagccgg cgcggttttt ctcggtcaac agcatggcta tcaccttcaa cctttttcac
    19561 aacgaggact ttatcttcaa tacgcctcgg gattttatgg atcgtttcga cggcgacccg
    19621 gaatacttca gcggcaaggg caaagcgcat ccaggccggg tttgggatac caactttatt
    19681 gctgacgcgc gcaacttcaa actagaagat tggaaggagc ggggcgcctc cggaaagaat
    19741 gtcatgctcg agatggccaa tggctcgatg gcggcgcaca tctccgagtt ccccgtaggc
    19801 acctataaga aagctcatcg acacggacct ggtttcaacg tcatgatcat caagggtcat
    19861 ggcttctcat tgttctggag acaaggagaa cagccgaaaa ggtacgattg gcaggacggc
    19921 agcgttttta ctccgcctgc gatgatgttc caccagcact tcaacaccgg cgccacaggc
    19981 gcgcgctatc tgccgccgcg cttgggcggg atcaagtaca gcttgggcga aagcttcggc
    20041 gacatcacca aggttgataa agacgttaag gccggcggca accagatcga gtattatgac
    20101 gaggatccga acatccgaaa gatgtttgaa gaagagctgg ccaaatccgg gacaaccacg
    20161 agaatgaatc cggatttcta caaaaaaccg gcctgactta tcgttgctcg ccggaatcgg
    20221 catcaatatc taagagccct gcatggtttt aaccgtgcag ggcctttttt tggccgatga
    20281 tcgtcgttat ttaactgagt tgtttggaat ccgggtagct tcgggtggaa atacttttaa
    20341 tcgtacttgg ctatttactg ggatcggtgc cggtgggatt cattgtcgga ttaaggtcag
    20401 ggatcgatgt cagggagacc ggtagtggta atgtcggcgc aacaaatgtc gccagggttc
    20461 taggcaagcg tcaaggtatc ttaaccctag tcgctgatac ggcgaaagga ttcctgccgg
    20521 tcattgtggc tatgcaactc ggggcgagcc tgtccgcaac cattttggtt ggcacggctg
    20581 cttttctcgg gcacctattc ccagtttttt taaaatttaa gggaggaaaa ggagtggcca
    20641 ccgccctagg tgttttcctg gcgctggctc caatagccac gctggtgttg gttgcgctgt
    20701 ttgccgttac ggttctagcc agcaggatcg tttccctaag ctcgatttta accgcagtag
    20761 cggcgccgat aatagtttgg ctgttttcct acccgccgtt agtagtcggc atgtctgctt
    20821 tcattgcgtt ggcaatcaca tgccggcatc gcagtaacat tcggcgaatg atgaacggaa
    20881 gcgaacctcg ctttggatcg gctagttctc aataatcact tccacccgcc gattttgcct
    20941 tcgaccgtcc tcggtatcat tgctggcgat cggagcccga gtgcctaatc ctgccgatgt
    21001 aagacgcccg ccgttgacgc cattacgctc cagtgattga gcgacggagc ttgcccgccg
    21061 ctcggacagc cgctggttgt aggcttcttg cgcggcgctt tcgcggcttg cgtggccttc
    21121 cacagtgacc ttgcgattgg gcgcctctcg ctgaagaata ttagcgattt gattcactcg
    21181 gtctcgccca tccacagtta aatcagcgct atcgaattga aagttgacgt tgggaagatt
    21241 gacggttacg cctcggctgg tttctcgcgc ctcgatgttg cgctttttta attcatcaat
    21301 cagcgcctgg tttttagcaa tttcagctcg ctgctgatcg atggcttttt gctgctccgc
    21361 ctgttttatc tcctggccct ggagttgatc gccaatgaga gctcccgtac ccaaaccgag
    21421 cgccccgccg accgccgcgc cgacgccggg ccgaccgacg gcagcgccga cgataccacc
    21481 cgcggccgcg ccgccgagcg cgccaattcc ggcgcctttt tctcgagtag ttaatgatcc
    21541 accggaacat ccagctccga tcatcgccaa tgcgatcaac gctgcggtaa aaaatctcgg
    21601 aagattgttc atagtaatct ttctcggcag taattcgggg ttagtattca gctgtttttt
    21661 tgagcctttc gagctcgcgt ttttgccgtt ccaactctgc ttggttggca tcgatctggc
    21721 gctgctgctc cgattgtttt tgttcctgcc cttgtaattg atctccaacc aatgcgcctg
    21781 atccaagacc tagcgcacca ccaatggctg caccagcgcc cggtcgtccg acagcggagc
    21841 caataatgcc gccgactgca gcgccgccaa gcgcgccaat gccggctccc ttctctcgtg
    21901 tcgttaaacc gccgcttgaa caaccgacaa cggcggatat cagacaaaaa ccgaccgtta
    21961 tcgtgagtgc gcgtttcata tggcctccca aagaattacg gcaaagaggc agtttgcagg
    22021 taaaacaagt ttagcaaatg gcctctgacc gggcaacttt ttcaaacgca atcacccaca
    22081 cttaaatgta tctattcggg tctttagact catctgtcag ggtcttgtta acacacccat
    22141 cctgttgaca tcatagacct tataagtcta gagtttaaga aaggagttcc attggaaagt
    22201 ttatctcagg aaagaattct cggcgtatta aggaaggttc aggaccctga acttcaccgg
    22261 gacatcgtaa gtcttggaat ggtgaagaat ttggtcctaa atgacggcaa ggtcagtttc
    22321 acagttgagt tgacgactcc ggcttgtcct ctgcgcgaga ctatcgaaac ggattgtaag
    22381 aaggcgttgg cggaagttgc tggaatcaga gacttggaaa taaatttcgg tgcgcaggtg
    22441 cgtggtagca aggcaggcga gggtcaaaca gatctgcttc catcagtaaa aaacgtcgtg
    22501 ctggttgccg ccggcaaagg cggtgtcggt aaatccactg tggcggctaa tctggccgtg
    22561 gctctgaaaa agcacggcgc ggcaaccgga ttacttgatg cagatattta cggaccttcg
    22621 gtgccgatta tcatgggcgt caaaggtgag ccgtccaaag tcgaggtcaa cggcgcgttc
    22681 aagattgctc cgacgatagc tcatggcgtg ccggtgatgt ccatcggctt tttcctggat
    22741 cccgaacagg ccgtgatttg gcgcgggcca atgctcggca aggcgctgca ccagctcatg
    22801 gctgacgtgc attggggaga actggattac ctggtcgtgg acatgccgcc tggaaccggc
    22861 gatgtgcaaa ttacgttttc gcaacagtta aaagtcagcg gcgcgctgtt ggtcgccacg
    22921 ccgcaagacg ttgctctggc cgacgtcatt cgtgccaaga ccatgtttga caaagttatg
    22981 atccccatcg tcggcgtcgt tgagaacatg agctatttca tctgcgacgg ctgtaacaag
    23041 aagcacgaga ttttttcccg cggcggtgct ttccgggctg cggagcggtt taatattccc
    23101 tacctggggg aaatcccgat cacgccggcg ctgcgagaag gtggagataa gggagtgcca
    23161 attctcattc aagatcctaa gtcggaagta agcaggatct ttcttgagat cgcggccaaa
    23221 ttggcgggac agctctcaat tgcttcggaa cgcgccaaac aaacgcaagg tctgaaaatc
    23281 gtttccagct agtctgaacc acaaatattt agccggacaa atccggaggg gtaggatcaa
    23341 tgattactaa agaacaagtc tacgaagcgc tgcacgaatg ttacgatccc gaaatcccgg
    23401 tcaatatagt cgacttgggc ttggtttatg atgtccaagt gaatgacgac aatgtcgcgg
    23461 ttaaaatgac cctgaccgcg cccggctgcg gcatgggcac gatgatcgct gccgatgcga
    23521 aagaaaagat tctcgctcta gaaggtgtga gtgacgccac cgttgatctg gtttgggatc
    23581 cgccatggga tccgagcaga attagtgacg aggccaagca gagactcgga atcggttaat
    23641 tactttcgcg atttgctgcg cttgcagcga tccggcgtac ctacaaaact cctgaagctt
    23701 gagcgcagtc agcttcgttc ttcacgatga atctttttcc ccgtttgcgg gccgttgagg
    23761 cctttcctgt tcaacaaaac ggcaacgatc tcatctatct caaagatccg ctcaatcttg
    23821 ccactccgtt gggagtttct cccatcggct attttctcat ggctcatttc gacggccgcc
    23881 attcttttat cgacatccaa gaaatctact cgaaacaatt cggtacggtc ttgctcaccg
    23941 atgaactcaa aagatttgtc gacatgctcg atgagcacta ttacctcctc agtgaacgat
    24001 ttacagagta tcagaagagt gtcattgaag ctttttccct agcgcccgcg cgggcgccgg
    24061 cccatatggg tggggtgtac cccaatgatg ctgttgccct gaaggctcag ctcgacggat
    24121 atttcaattc accggaaggc cccggtctgg cgttgacaaa cggcaggagg atgaccccac
    24181 aggcaattgt cgctccgcac atcgatttcc atcgtggcgg ctccgcctat gcgtgggcct
    24241 ataagccgct gctggaaagc gatggagctg acttgtacat cctgcttgga acttctcatt
    24301 gcggcggtaa cactcccttc attctaacgc taaaagattt tgaaactcct cttggcattg
    24361 tcgagaccga caaaacgttt gtttcacatc tacagaatgc ttgtcagcag gatctgttcg
    24421 cagacgagta tctccatcgc ggtgaacatt ctgtcgagtt tcaggtgctg ttcttaaaat
    24481 acctggcgca gcggcgcgcc gaattactag gccagcccga gcgacctttc aagattgtac
    24541 cgatcctggt ttcatccttt catgagatgg tggcgaccaa aaccctgcct gaaagaactc
    24601 cgacggtcgg aacatttctc ggcgcgctca agagcttggt tgacagcgag agacgagaag
    24661 tgtgttttgt cgctggcgtg gatctagcgc acgtcggacg ccagttcggc gacggtgagc
    24721 cagtcacgga ggattttctc aaatgggtcg aagccgagga tgatcgtctg gtggagcgtc
    24781 tggccgctcg tgatgcgccg ggatttttta acgagatcgc aaaggatcac gataaaagac
    24841 ggatctgcgg gttctcaccc ctttactcgc tgattcactt gctcaagaag ggcaagggac
    24901 aacgattaaa atatagccaa tcctttacgc ctgaaacagc ctcggcggtg acctttacca
    24961 gcatgatctt tgactgatcg ggatgagaag cggcgaccgt tgtgcttact cccggttctc
    25021 cgatggtgtc tctgggtcgg ttcatcacgc tcaatgcata ttcatgccgc catcgacgct
    25081 aatcgactgg ccggtcatga agtcgctatc atcggaggca agaaagatca ccgtgcctac
    25141 cagatcctga gggtactgat cgcgctgaat cgagcgacgc tttcttcggt ctgccaattg
    25201 ctcggcggtc atgacgccct cttgattcgc gcctgaaatg gtcagacccg gcatgattga
    25261 attaacgcag atgttgtcgc cgcctacctc ccgtgccagc gcccgagtga agccaatcac
    25321 gccggccttg gatgatacgt aatggagaaa gaatggcgag ccgttcaagg ttgttccgga
    25381 tgaaatgttg attattttcc ctttgccctg ttgtttcata gccggataaa ccgccttgac
    25441 gcagtgaaac agacccttaa cgtttactgc catgacacgg tcccactcgt cgctgtcgat
    25501 ttccatgaag tttttctttt tcagcgctga atagagtccg gcgttattga ctaggatgtc
    25561 gattcggcca taggcggtaa gcgcggcttg agccatggct tgcgctttgt cgaatgaagt
    25621 cacatcaact gcgaccgcca tcgcttcgtc gccggattgt ttgatgtcgg tggcgacctt
    25681 tttcgcttcc gcttcttgaa tatcggcgac gacgaccttg gccccttctt tggcaaggcc
    25741 gtgagcgtat gctttgccga tgccgacgcc gcccccggtg acgatcgcga ctttatcttg
    25801 taggcgcatg tgagtttcct ccgttttaaa tctgatcaga tctcgagccg gtaaaagcgt
    25861 gcggggttat cgtgcagaat cgctttcttg tcttcctcgc cgatgtccgt gcgtcctaag
    25921 aattcgggta agtcgtgact gaacatgtca ggcagacgct catggggaaa atccgacggc
    25981 cacatcaagc aagcaggatt gaattgcttg atgacctgcg ggagcgcttg ctcttcaact
    26041 tcacaagtag cccaaacctg acccgatcga atgatctcac tcggctcttg cttgagcaac
    26101 ggcgcctgga agcgatacgg cttctccatc tcttcatcca tgcggtccat catgtagggt
    26161 aaccagcccg aaccggcttc gaggaatgcc actctcagct gaggaaagcg gctgaacaca
    26221 ccttcgaaca ccatgtgcgt gaactggatc aggatcgcaa aggggtgttc cagggtatgc
    26281 acgtgaagaa acttgttgaa gaagtcgaat cccatgccgc ggctcggcgc gccatggacc
    26341 gtcagcggca tatcaagttt ctcagcggct tggtagatcg gatcgaaatc ggtatgtccg
    26401 tagcctttgt tcaggaccgt caccgccggc aggagcccgg cgactagtcc gagcccttta
    26461 gcccgctcca gctcctttgc cgcttcgatc ggctcgtgca ccggcagaag cgctaccgct
    26521 ttcaggcgcg aactttgttt ggtgaagcgc tcggtgatcc atgagttgta agcgcgcgcg
    26581 atcacacagg accactcagg gttctgaatc aatccaagcg acagaccgcc ggtggggtag
    26641 agcacggtgg tgtgcacacc aagctcgtcg agaaattcga gccatttggg cgcgggtgtc
    26701 tccgggacct tgccgggaac gccgaatccg cggttccagc catcaaggga aggaaaataa
    26761 ctataggtct tgaagtgcgg accctgtcga taacgaccgt caagaaactc ccgcagctca
    26821 gcgtcagtct ccatcacatg gccatcggca tcgatgatcg gatagttaac gttcattcgg
    26881 ttattctccc gtggttccgg tgcgcagcac ctgcattgtt ggcggcgtat cgggtggtcg
    26941 cattgctgaa gaccggatcg gcttatctct cactcgggta aatcggttgc gcaaaggcgg
    27001 accggtcagg atcttactac gaaggccacg aagttagata agttataaga gatcaatcat
    27061 ctgaacgatt cggtgcttcg tgataaatct ctgtttcgct ttgtttcggc tattgcgccc
    27121 aactcatttt ggttaaagcc ctatctatcg atcatcaccg gttcggacgg agccgctcgg
    27181 ttatacaaac gagatgcctc catctacatt cacggtttgg ccggtcatga agtcgctcag
    27241 tggcgaggcg agaaacaaca tggcgccgac cacgtcttgc ggcatctgaa tccgcttcaa
    27301 cgcgcgcaag ccggcggagg attcgcgcag acgaatgacc tcttggcttg gattttcttc
    27361 agagagggta ttacccggcg ccaggacatt gacattgata ttgtcatcgc cgacctctcg
    27421 agccaacgtc cgggtaaagc cgatggttgc cattttagat gtcacgtagt gaattcttcc
    27481 ggcacttccc gcgaacacag ttcccgaggc gatattgatg atcttgccgg atttctgcgc
    27541 ccgcatactt ggcagcacgg cgcggcaaca gaaaaacagc ccttttaggt tcacagccat
    27601 gagccgatcc cactcggcgg gatcgatggt ttctatgcgc ccgcgattca tggggatggt
    27661 cgcgaagatc gctgcgttat tgatcagaac atcgattctg ccgaaacgct gcaataccgc
    27721 cgcagccatc tccttggttg atttctcgtc ggcaacatcg gtgtgaagcg ccagagcttg
    27781 cgcgccattc tgctgcatga cttctgcgct gacttgatct gctgccggct tgtcgatgtc
    27841 agccaccacc acgcgcgagc ctgcttgtcc gaagccgagg cagtatgctt tgccgatgcc
    27901 gtggccgccg ccggtgacga tcacgacttt gtctttgagt ccctcgacca tcttctagct
    27961 cgggatctct ggatccatta tcccaatgcc ggatcgatca gcgctttggt cgggaacatc
    28021 tcgggaatct caccgccgac tgcgcgaatg cacttttcga tttcatttag cggaaatgta
    28081 tggctgacca tttcttgcac cgggaacttc gtctcttcaa tcagccgcag cgcggcctcg
    28141 gtggcgtcat tgtctgcggt gaaacaaccc tgcagcttga tctcattcca aactagttta
    28201 tccatgagca tcggtgtcac tgttgtatca ccggttagtc cgcccagaac catagtcgat
    28261 tgccgatgca gacaatcaac cgattttaag atcgcgttcg gattgccgga tacatccacg
    28321 accacgtcgc acatctcacc attggtttcc ttgcgaacga cctcgacgac gtcttcttct
    28381 tcgacgttga tggtacgggt ggcgccgaac ttcttggcca actcaagccg cggttggtcg
    28441 cggccgatgc cgctgataaa aacttttgcg gcgccggcat ggcgcgaggc aaatgtgcag
    28501 gagagccctt gttggccggg accttggatc aaaacgaagt cgcccatctt cacgccgccg
    28561 cggcggatcg cccattgaaa gccgttggcc attacagagc cgatcaacgc cgccgcttcg
    28621 gcgggcaacg catcgcttac cttggtcgca aggacatcag gggccaggta aacgtaatcc
    28681 gcaaaaccgc cgaacaggtg tggcggctgg tcgcatttgg ttcgaccgcc atagttggtc
    28741 cgctggcggc aaaagcgctg gttgttgcga cggcaatccg agcagcggcc gcagccaagc
    28801 gcgcctttca gaatgatacg atcgccttct ttgacgtgat ggatcgcggc ggcgtttttg
    28861 cccagcttct caactcgacc gagaatttca tgcccgagaa tgatcggata gtcgactttg
    28921 agcttcccat gcaggtactt cacatcggtg ccgcatacgc cgcccatttc aactttaacc
    28981 agcccgccat cggcagggat gtcaggcatt ggatactcgc gaatctccaa cttatccgga
    29041 ccgagaagcg ttgcgcctcg gactgtcgcc ataaaaacct cctcacattt ccgcgtttag
    29101 tatgctccgg atctgtattg ccttgttact tgactttata ttccttggtc aacttgtcga
    29161 taaaaccgct ggcatcgagt tctttgacca atgactgatc ggcgaaacgc tccggatctt
    29221 ccttgaggac cttgggatct ttcttaccga cttcctggat cggtaattca tagtattctt
    29281 tggcagggta gggcttcaga ggaaagtcat attcgcgaaa attttcctcg atcacctccc
    29341 ggtcctggac tttcatgtat ttgccgatca ctttcatgct gaactctttg ttggctttat
    29401 aataatggac gccttcgatc atggctttgg tgaagttcac cagatcggcg cgatttttca
    29461 tcaggtaggg gcggcgtgaa acgaagaaca aactggaata aggaatgccg agcttggatg
    29521 cgtcggcgat cagggaaaat ccgagcttgc gcgcttgcaa ggtcatgggg ggagtgagca
    29581 tcgcggcatc cgcgttggcg gtctctacgg cggcaattcg attggcctga ttgccgacct
    29641 gcagtaacgc cacatcggta tcgggtctga gtccttcgcg ctgcaagaga tagcgtgcag
    29701 caaagtcggc attgctgccg aagcggctga tggcgatgcg tttgacttcc ttaaggctct
    29761 tgattccttt tctggcgtag atgcgattgt ccatggtatt gccaagccgg ccaaacatga
    29821 ccagctccaa cgccggcgtt gaaattacta cctgcggtga aaacatgccg acttgcgcgt
    29881 cgtcggatga caacgcggcg gcggcggatg cagcgccttg gatgaagatg agctgaactt
    29941 gattgccgta cttttcaaac agtttggctt ccttggttat ccagagcggc gcgaactggc
    30001 ccacttcgct ggtccagacg acattgatgt ttgccgcacg cgcaacggct ccttggcaca
    30061 gcgctccgag aacgatcaag ctcccggcta aaaagtgacg gccgtatttc attcgcagct
    30121 cctttgcatg tccgacaaca agattgacgc cgatggctta atcctatact gcgttagatg
    30181 cctgagaatc aagtaaggga gagcccaatg agccgactct tgtttgcatt tatttttgtg
    30241 ctgctgtcga ccagtgatgt cacagcgcaa ggattgactc gggtgatgtc gggatacgcc
    30301 gccacgtcag gaccgcatgc agtcctttgg ctggctcgcg aagccggtct cttcgagaaa
    30361 aacggtttgc gcactgacgt cgcatacata cgcagcggct cgaccatggg gcaggcgtta
    30421 gtcgccggag aaatccagtt agcgcaaatg ggcgggcccg ccgctcttgc cgcaggcgtc
    30481 gctggtttcg acatcacgat ggtggcggtc gctctgaata caacgcccat cgtgatcatg
    30541 ggcaaagttt cgaaaatgga agagctcaag ggcaaagcga ttggggtgac gcgctacggt
    30601 tccaataccg atatatctgc tcgcttcgcg atcagaaaag ccggaatgca accggagaaa
    30661 gatgtcgctc taatccagct ggaagactat gcaggaatca tgggcggcat tcagtccggg
    30721 cggattgccg ccggcgcgct ggctgatccg tttaccgatc atgccaagag gttgggctat
    30781 aacgaaatcg ctgacatcgc cgcgctgggc cttgagtttc cgtttgtcgg gattgtcacg
    30841 aaaaaaactt tcatcaagga acagccggac gttgtccagc gattcgtgcg cgcctatacc
    30901 gaatccatcg cactgtataa aagcaaccgc gaactggcga agaaggtgac cagcaaatac
    30961 acgggtatca aagacccgga aatcctgaac tccacggtag atttttacgc gcccaagtta
    31021 gccagcgtgc cttatccgac cgtcggcgga atacgatttg tattggatca ggttgcgact
    31081 cgagatcccc gcgccaagaa cttcaatcct gagactttca tggatgtgcg gtttgtaaag
    31141 caactcgaag acagcggatt tatacaggga ttgaaacgga agtagtgatc gatctgtttg
    31201 gttagtatcc attcttcgcg agaccgactc tacgacatcg tccctgatat gcgcctggcc
    31261 tgacagcg
//
LOCUS       DASUVS010000186         6826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_732015, whole genome shotgun sequence.
ACCESSION   DASUVS010000186 DASUVS010000000
VERSION     DASUVS010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732015"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VF182_10460"
     CDS             complement(<1..193)
                     /locus_tag="VF182_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6437543.1"
                     /translation="MKLPIYMDNHATTQVDQRVIETMLPFFTEKFGNASSRNHVFGWE
                     SEAAVDHAREQVASLIHAAS"
     gene            complement(332..2857)
                     /gene="uvrA"
                     /locus_tag="VF182_10465"
     CDS             complement(332..2857)
                     /gene="uvrA"
                     /locus_tag="VF182_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX6437544.1"
                     /translation="MRGEIVVRGARVHNLKNIELKIPREQLVVITGVSGSGKSSLAFD
                     TLYAEGQRRYLESLPVNHRQLLQQLDKPDVDSIDGLSPAIALQQRPANLGPRSTVGTM
                     SEVQDFLRLLFTRVGQPYCTHCGARISVQTTEQIVDQLAALPNGARIVLMAPIYPSRK
                     IAANALLREFAGQGFTRVRIDGQLQELDEEKHLEPDRTYQIELVIDRLTIKEGVEKRL
                     ADSLETASRVGQQVVRIEVQADSAPSKELRFSQKFACAECGSSFPEIVPSLFSFNSPN
                     GACPACAGLGGLERASRKPKTETEATDMALCKECNGARLRKESLAVRIGGKNIAQLAA
                     LPIDDAIQYFDALQFQPQERITARKILAEIIPRLGFLKQLGLDYLTLDRLSSSLSGGE
                     LQRVRLAKQIGSKLSGVLYIFDEPSIGLHQKDNAKLMELLQELRAAGNSILIVEHDPE
                     TILSADYIVDMGPGAGVRGGEVVAIGSPDEFMTQTHSLTAQYLSGRRKIPVPLLRRKG
                     SGEFVILKGARQNNLQDITVEIPIGVVTCVTGVSGSGKSTLVMDILYNEVSRRLRRYR
                     TKTGALDDLIGWQQFDRVVGVDQSPIGRTPRSNPATYAGIYDPIRELFAQLPESRMRG
                     YQANRFSFNASGGRCEACGGEGLMRIEMYFLADVLVTCDICKGRRYNRETLDVKYKGY
                     SIADVLELTVIQALDLFAHIPAIAERLHALIRVGLGYIQLGQAAQTLSGGESQRLKLA
                     KELARRSSGRSLYVLDEPTSGLHYADVEVLLELLHRLTDAGNTLVVIEHNLEIIKNAD
                     YVIDLGPGAGKSGGRVIARGTPEEVAAVSQSHTGRYLTKVLTG"
     gene            complement(2866..4221)
                     /locus_tag="VF182_10470"
     CDS             complement(2866..4221)
                     /locus_tag="VF182_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX6437545.1"
                     /translation="MNPSIFREYDIRGVAEKEFDEDFAFLLGKVHGTIVNEAGGARVS
                     VGRDCRATSDRYTQAVIDGLVSTGLQVVDIGVCPTPLLYFSLFHLDTDGGIEVTASHN
                     PPEYNGFKLCLGKDTLYGAQIQNIKARIEQNHFVEKPGGRVEGYEIVLPYQQHLLRDV
                     PKLKRLLKVVVDAGSGVGGPVAPPIFRHLGCTVWEIACTPDSNFPVHHPDPTVPENLE
                     LLIQKVRAEGADLGIAYDGDADRIGAVDEQGNIVWGDELLMIFARDVLQRNPKATIIS
                     EVKCSQRLYDDIVKRGGKPIMWKAGHSLLKAKLKETNALLAGEMSGHMFFKERYFGYD
                     DAIYASLRLLEIIANSDGPFSALLADLPRTVSTPEIRIDCPDDRKFIIAERAKEYFRR
                     HYDIIDIDGVRVRFPEGWGLIRASNTQPALVLRFEAQTAEKMLEYRALVEGKLKELEI
                     S"
     gene            complement(4218..5213)
                     /gene="pdxA"
                     /locus_tag="VF182_10475"
     CDS             complement(4218..5213)
                     /gene="pdxA"
                     /locus_tag="VF182_10475"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX6437546.1"
                     /translation="MGDPAGVGPEVILKALSHQAIGRTCNPLILGDWGVLQRVRARSK
                     RLPKLISWQSGMPLLPLLRGAGGFVVCPLSTLRENESRPGRPGKAGGHAAYRYITVAA
                     RLALSSVADAIATAPISKSILIDAGYNYPGHTELLAELSRTRECRMMLIGKKLRVVLV
                     TGHVGYTKVVRALTSDGIRTTLELTHRALINSFGIARPRIAVAALNPHGGEEGIFGSE
                     EKEIIVPGIQAARRKGFPAFGPFPADSLFHKAVRGEYDAVVCMYHDQGLIPLKLHHFF
                     GGVALTLGPPFIRTSVDHGTAYDIAGTGRADETSMAQAILLAADLARQKIKSRDH"
     gene            complement(5290..6621)
                     /locus_tag="VF182_10480"
     CDS             complement(5290..6621)
                     /locus_tag="VF182_10480"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6437547.1"
                     /translation="MLMLGRALKNSLPILSFLIFLSPFSVQGRVVEQIVTVIDGEPYT
                     LTNIASYAKTKMAREFPVGSLEKINGSDREVLEQFITEKLLDAEIREAGIKVTDQDIE
                     IYIDQIKQKNRLTDADLKTALGREGQSLQSYKASVRAELEKGELINRQVRKKVNITDE
                     DVERYYKLNSNNYRVEDRARIRHILLPLSDKASPEEVQVALAKGNELYDQIAAGGDFA
                     GLAQKHSDGAGRESGGDIGWVNRGTLLKPIEDAAFDKLSVGQVSRPIRSSMGLHLVKL
                     ESRDIGAVLPLSKVASKIKEELYAKAMEERYSKWLKTELRRKHTVDVKLAGVVFKPED
                     SKEDTMGSLMAKSTRLNRKEDRGFLAYLNPLSYVVSETPSNEEEQKQGPLAGKNIVSV
                     FGVPLFTTDRVDDVPDILSPPAQPSEKNSPAQEPQSGFFSSIVNRLNPFKR"
BASE COUNT         1570 a         1879 c         1751 g         1626 t
ORIGIN      
        1 gagatgcggc atggatcaaa gaagccacct gttcacgcgc atgatctacc gccgcttcac
       61 tctcccaacc aaagacgtga ttgcggctcg aagcattgcc gaacttctct gtaaaaaacg
      121 gcaacatcgt ctcgataacc cgttgatcca cctgcgtggt ggcgtgattg tccatgtaaa
      181 taggcaactt catagacagg tgctgaaatc tcagcttatc ccactacttt ggtctgattt
      241 aaagataact aatccgatag tgtcagtcaa tgagtcgatc ggggaattat ctggaatcag
      301 gaactcggag ctagccaaaa acgtcgagaa tttagccggt aagcaccttg gtcaagtatc
      361 ggccggtgtg tgactgcgat accgcggcga cctcttcggg cgttccacgc gcaatcactc
      421 tgccgccgct cttcccggca cccggcccta gatcgatcac gtagtcggcg ttcttgatga
      481 tttccagatt gtgctcgatc acgaccaagg tgttgcccgc gtcggttaaa cgatgcagca
      541 gttctaggag tacctcgaca tccgcgtagt gaaggccgga agtgggttca tcgagcacat
      601 agagagaacg tccggaggat cgtcgcgcca gttctttggc gagcttgagt cgttgcgact
      661 cgccaccgga aagggtctgg gctgcctgcc cgagctggat ataacccagg ccgacccgaa
      721 tcaaagcatg aagccgctcg gcaatagccg gtatgtgagc gaataaatcg agagcctgga
      781 ttacggtgag ttccagaacg tcggcgatgc tgtacccttt gtatttaaca tctagggttt
      841 cccggttata gcgacggcct ttgcaaatat cgcaagtaac aagcacatcg gccagaaaat
      901 acatctcgat gcgcatcaga ccctcaccgc cacaagcttc acaccggccg ccactggcgt
      961 taaatgagaa ccggttagcc tgataacctc tcattcgcga ctccggcagt tgtgcgaaaa
     1021 gctcgcgaat cgggtcgtag attcctgcgt atgtggctgg attcgagcgt ggcgtcctgc
     1081 ctatcggcga ctggtcgacg ccgaccaccc gatcgaattg ctgccaaccg ataagatcat
     1141 ccagggcccc tgtcttggtg cgatatcgcc gcaggcgccg ggaaacttca ttataaagga
     1201 tatccatcac taacgtgctc ttgcctgagc ccgaaacccc ggtcacacaa gtcaccacac
     1261 ctatcgggat ctcaaccgtg atgtcctgca ggttgttttg tcgcgctcct tttaaaatta
     1321 cgaattcgcc ggaacctttt cggcgcaata gcggaaccgg aatctttcga cggccggata
     1381 aatactgagc cgtcagcgag tgcgtctggg tcatgaactc gtcgggcgac ccgatcgcaa
     1441 cgacctcacc gcctctaact ccggcccccg gtcccatatc gacgatataa tcggcactga
     1501 gaatagtttc cggatcatgc tccacgatca agatagaatt gccggcagcg cgaagttcct
     1561 gtaaaagctc catcagtttg gcattatctt tttgatgcag cccgatgctg ggctcgtcaa
     1621 aaatgtaaag aactcccgat agcttcgagc cgatttgctt agctaagcgg accctttgca
     1681 attcgccccc agacaaactg ctcgatagcc gatccagggt cagataatca agtccgagtt
     1741 gctttagaaa accaagccgg ggaatgatct ctgcgagtat ttttcgtgca gtgatgcgct
     1801 cttgcggttg aaactggagc gcgtcgaaat attggatggc gtcgtcgatc ggtaaggcgg
     1861 ccagctgagc gatatttttg cccccaattc ggaccgccaa gctctctttc cttaggcgag
     1921 cgccgttaca ttccttgcaa agcgccatat ccgtggcctc agtctctgtc tttggctttc
     1981 gagacgcccg ctccaacccg cctaacccgg cacacgccgg acaggcgccg ttcgggctat
     2041 taaatgaaaa caggctcgga acaatctccg gaaaggaact gccacattca gcacaggcga
     2101 acttttggct gaagcgtaat tctttggacg gcgccgaatc tgcctgcacc tcaattctta
     2161 caacctgctg gccgacacga gaagcggttt caagcgaatc tgctagacgc ttttccacgc
     2221 cttctttaat cgtcaaacgg tcaattacca gttcgatctg gtaagtccgg tctggttcca
     2281 aatgtttctc ttcgtccagc tcctgcaatt ggccgtcgat cctgactcga gtgaagccct
     2341 ggccggcgaa ctcgcggagc agtgcattgg cagctatttt tcttgacggg taaataggcg
     2401 ccataagaac gatccgggcg ccgtttggca gagctgcgag ttggtcgact atctgttcgg
     2461 tagtctggac cgagattctc gcgccgcagt gagtgcaata cggctggccg acacgagtga
     2521 acagcaaccg caaaaaatct tgcacctcgc tcatcgttcc gacggtagag cgtgggccca
     2581 agttcgcggg tctctgttgg agcgcgatgg cgggagaaag tccgtcgata gaatcgacgt
     2641 cgggtttatc gagctgctga agtaactgac gatggttgac gggtaacgat tcgagataac
     2701 ggcgctgacc ttcggcatac agtgtgtcga aggctagcga cgatttacct gaaccggaaa
     2761 ctccggtgat aacgactaac tgctcgcgcg gaatcttcag ctcgatattt ttgagattat
     2821 ggacacgcgc ccccctgaca acgatctcgc cgcgcattat gggtactatg agatctccag
     2881 ttccttaagc ttgccttcga ccaaggcacg atattccagc attttctccg cagtttgagc
     2941 ctcaaaacga agcactagcg cgggctgcgt attcgatgct cggatcagac cccatccttc
     3001 ggggaatcgc acgcgcacgc cgtcgatgtc gataatatcg tagtgccggc gaaaatactc
     3061 ttttgccctt tcggcgatga taaatttcct atcatcgggg cagtcgattc ttatttccgg
     3121 tgtcgataca gtccggggca ggtccgcaag caaggcggag aaaggcccat cagaattggc
     3181 gataatctca agcaaccgca acgaagcata aatcgcatcg tcataaccaa agtagcgctc
     3241 cttaaagaac atgtggccgc tcatctcacc ggccaacaag gcgttagttt ccttcaactt
     3301 agccttcaac agcgagtggc cggccttcca catgatcggt ttgccaccgc gcttaacgat
     3361 atcgtcgtag aggcgttgcg agcatttcac ttcagaaatg atcgtcgctt tggggttacg
     3421 ctgcagaaca tcgcgtgcaa aaatcattaa cagctcatca ccccaaacga tatttccctg
     3481 ttcgtccacg gcgccgatac gatcggcatc cccatcgtaa gctataccta aatcggcccc
     3541 ttccgcgcga actttttgga tgagaagctc gagattttcg ggaacggtag ggtcgggatg
     3601 gtgaacggga aaattactat caggcgtaca ggcgatttcc caaaccgtgc agcctaggtg
     3661 acgaaaaatt ggcggcgcca ccggtccacc aacgccactt cctgcatcga ccacgacctt
     3721 gagtaagcgc tttagcttgg gaacatcgcg caacagatgt tgttggtaag gtaggacgat
     3781 ttcataccct tcgactctgc cgccgggctt ctcgacgaaa tgattttgct cgattctggc
     3841 tttgatgttc tggatttgcg cgccgtacag cgtatcttta ccaaggcaaa gcttaaatcc
     3901 attgtactcg ggcgggttgt ggctcgctgt gacctctatt ccaccgtcgg tatcgagatg
     3961 gaatagcgaa aaataaagca agggggtcgg acagacacct atgtcgacga cttggagacc
     4021 cgttgatacc agcccgtcta tgacagcctg ggtgtaacga tcggaagtag cccggcagtc
     4081 tcggccaaca ctgacgcgag cgccgcctgc ctcattgaca atggtgccgt gaactttgcc
     4141 aagcagaaaa gcaaaatctt catcgaactc cttctcggcc accccgcgga tgtcgtattc
     4201 gcgaaagatc gatggattca atgatctcta ctcttgattt tttgcctggc taaatctgca
     4261 gcaagcaaaa tcgcttgcgc catgctggtt tcatcggccc ggccggtccc agcgatgtca
     4321 taggcagtgc cgtgatcgac ggaagttcga ataaacggag gccctaaggt gagcgccact
     4381 ccgccaaaaa aatgatgcag cttgagagga atcagtcctt gatcgtgata catgcagact
     4441 accgcgtcat attcgccgcg caccgctttg tgaaaaagac tatcggccgg aaacggccca
     4501 aatgccggaa atcccttgcg ccttgcagct tgaattccag ggacaatgat ttctttttct
     4561 tcgcttccga aaattccttc ttcaccgccg tgcggattca aagccgctac agcgatgcgt
     4621 gggcgtgcga tcccaaacga attgatcaaa gctcgatggg tcagctccag ggtcgtccga
     4681 atgccgtcag acgtcagcgc gcgcactact ttggtatatc ctacatgtcc ggtcaccagg
     4741 acaactcgca gttttttgcc gatcagcatc atgcggcatt cgcgggtccg gctgagctcg
     4801 gcaagaagtt cggtatgacc cggatagtta tatcccgcat cgatcagaat acttttgcta
     4861 atgggggccg tcgcgatcgc atcggctaca ctcgataagg ccagccgggc tgctacggtt
     4921 atatagcggt aggctgcatg cccgcctgct ttgcccgggc ggcccgggcg cgattcattt
     4981 tcccgcaagg tcgacagcgg gcagacgaca aagccgccgg cgcctcgcaa caaaggtaac
     5041 aacggcattc cggattgcca ggaaattaac ttgggcagcc gtttacttcg agcgcggacc
     5101 cgttggagaa ctccccaatc gccgagaatc aggggattgc aggttcgtcc aattgcctgg
     5161 tggctgagag ccttcagaat gacttcagga cctacccctg cgggatcgcc catcgttacc
     5221 gccactatcg gttttcgaga cataagctca gatggccggg atcggggtgc cggacagaga
     5281 aatagaggcc tagcgcttga agggattcag ccggtttaca atggatgaga aaaaaccgct
     5341 ctgtggttct tgagctggcg aatttttttc gctgggctgc gccggtggag agagtatatc
     5401 gggaacatca tcaactcggt cggtcgtaaa aagcggcacg ccgaagacgc taacgatatt
     5461 cttaccggct agcggtccct gtttttgctc ttcttcgtta gacggagttt cgctgaccac
     5521 gtacgaaagt ggattcaaat aagctaagaa cccgcggtcc tcttttcggt tgagccgggt
     5581 cgacttcgcc atcaaggatc ccatggtatc ttccttcgag tcttccggct taaataccac
     5641 ccccgctaat ttgacatcga cagtatgctt gcgtcgcaac tcggttttga gccatttaga
     5701 gtaacgttcc tccatcgctt tagcgtagag ctcttccttg atcttggaag caactttaga
     5761 cagtggcaga accgcgccga tgtcccggct ctcgagtttc accaaatgta accccatgct
     5821 gctccggatt gggcggctga cttgaccgac agagagtttg tcgaatgcgg catcctcgat
     5881 aggtttcaac aacgttccac gatttaccca gccaatatcc ccacccgatt cccggccggc
     5941 gccatcggaa tgcttttgcg ccaacccggc aaaatccccg ccagcggcaa tttgatcgta
     6001 taattcgttt cccttggcta gcgccacttg tacttcttcc ggggaagctt tgtccgacag
     6061 tggcagcaag atatgacgaa tccgagcgcg atcctcgacc cgatagttgt tcgaattcag
     6121 cttgtaatat cgttcgacgt cttcgtcggt gatgttgact ttttttcgca cctgacggtt
     6181 gatcagctcg cctttctcga gctcggctcg aactgaggct ttgtaagatt gcaagctctg
     6241 tccctcgcgg cccaaggcgg ttttcaaatc cgcatccgtc agacgattct tctgcttgat
     6301 ctgatctatg tatatttcga tgtcctggtc ggtaaccttt atgccagcct cgcgaatctc
     6361 tgcatcaagc agcttttctg ttataaactg ctctagtact tctcgatcgc tgccgttaat
     6421 tttttccagt gacccgaccg gaaactcccg cgccattttg gttttggcgt aggaggcgat
     6481 gtttgtcaga gtgtacggct ctccgtcaat caccgtgaca atctgttcaa cgactcggcc
     6541 ttgcactgaa aacggactta aaaaaattag aaaagatagg atcggaaggc tgtttttgag
     6601 ggcgcgcccc agcataagca ttctacctcg acagatggtt tgttgggagt acagtttaaa
     6661 tcaggcctaa cacgatcccg cgaaaaagcc aaacctgtga cttaaacatt gatgctaaac
     6721 atgctccgat atcgcaacat tatgcagcgc ttccgaacgg agtgatagat tcgccggcgg
     6781 ctcatagatg ctatggagca attcttccac ctccgtaatc aatgat
//
LOCUS       DASUVS010000187         4865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1606351, whole genome shotgun sequence.
ACCESSION   DASUVS010000187 DASUVS010000000
VERSION     DASUVS010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606351"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..740)
                     /locus_tag="VF182_10485"
     CDS             complement(249..740)
                     /locus_tag="VF182_10485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6437548.1"
                     /translation="MDDITIRKAAPEDAEAACAVLVRSIKEICAPYYENDEEILAQWL
                     ENKTPANVRQWIDSDGSHCVVAVNLHGQVLGFSSMYGAEIMLNYVLPEALHQGIGKAM
                     LKALEAQAIASGIDHIRVLSSIPARTFYERNGYVSNGAPRHVGRILGDFPLIKRIPRK
                     HRP"
     gene            complement(806..1360)
                     /locus_tag="VF182_10490"
     CDS             complement(806..1360)
                     /locus_tag="VF182_10490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEX6437549.1"
                     /translation="MTERDKMLAGELYDPLDPDLVAARERARDLCQGLNQTRESEREL
                     RRSLLCDLFGAGGDTVWMQSPFYCDYGSNIYLGTRIFFNFNCIVLDVCEVRIGDYTLF
                     GPGVQILTPVHPFNAVLRRGQESGKPIEIGADVWVGGGALILAGVHVGSRAVIGAGSV
                     VTRDIPAGVFAAGNPCRVIRDILE"
     gene            complement(1564..1740)
                     /locus_tag="VF182_10495"
     CDS             complement(1564..1740)
                     /locus_tag="VF182_10495"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6437550.1"
                     /translation="MCGWLKDKYGVSWQIVPRVLIEMINDPDSEKSVKVMEAMLLMKK
                     IDIEKSKRAYTGSV"
     gene            complement(1751..1969)
                     /locus_tag="VF182_10500"
     CDS             complement(1751..1969)
                     /locus_tag="VF182_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437551.1"
                     /translation="MSNDMHGGKTITGKTENGGGRRVGGWRIAPWAAAALVLLLPLIA
                     MQFTDEVNWDVADFLFAGALLVGVGGRG"
     gene            complement(2102..2503)
                     /locus_tag="VF182_10505"
     CDS             complement(2102..2503)
                     /locus_tag="VF182_10505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6437552.1"
                     /translation="MRLPKWVVRIANEITNLALLVGIPRPPYTRENALIVETAGRRSG
                     KRRRIPVGYLDDGGRIIVVVEDGPRADWVRNALANDGRLRIHLRGAWHDARLQIVEAD
                     PANYLSRMNRVHAGFVRLESTTPAVVEIVPE"
     gene            complement(2614..2880)
                     /locus_tag="VF182_10510"
     CDS             complement(2614..2880)
                     /locus_tag="VF182_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437553.1"
                     /translation="MDEAQISKVAAVLTEWNPLGSGADRIADLDGYRIEAIDIIMAIR
                     VFPRFATPEEAVSDILNQAFDLSLKPAECMAPARQIESILRPDQ"
     gene            complement(2973..3392)
                     /locus_tag="VF182_10515"
     CDS             complement(2973..3392)
                     /locus_tag="VF182_10515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEX6437554.1"
                     /translation="MTVAHRQLTAKERQILEKQVLVHVGIVLPDGTPHVSAMWVDLDG
                     EDIILNTAEGRTKANVLHLGSPVALSVLDPENPYRNLGLRGRVVEVITEDRGAGAGIN
                     RLAKKYLGVEEYPYRAPGERRVQFRIRPEVVSGWGKE"
     gene            complement(3898..>4865)
                     /locus_tag="VF182_10520"
     CDS             complement(3898..>4865)
                     /locus_tag="VF182_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6437555.1"
                     /translation="QAAANYMGAKPDEIALVDSTTEGLALIYNGMPLKRDDEILTTTH
                     DHYVHHEAIRLATQKLGAVTMDEVVTNLRRAIGRKTRVVGLTWVHSSTGVKLPIRQLA
                     AVVSEANGKRGEKDRILLVVDGVHGFGSQDESPVDMGCDFFVAGTHKWIFAPRGTGIV
                     WGRRDQWARIRPTVPAFHGLAVHAWMEGKDPGPTQASFVSPGGFKAYEHQWAMPEAFA
                     FHRSIGRKRIADRIAALNTQCKEGLAGIPLVKLHTPHSPELSAGIICFEIAGMAPDEA
                     VPKLLDKKVIASRSPYKVSYARLAPSLVNDEEQVEHALRAVRALS"
BASE COUNT         1033 a         1454 c         1303 g         1075 t
ORIGIN      
        1 cggcatatcg aattctccaa actcctcact attctttttc taaagactgt gctgcgcggc
       61 gtggctgtac aagcggctca cgtctagggt gaagcttagc tgagccgcca aaacgtgtcg
      121 caacgcaact tccgaggatc aatgaaattc caatcaccgg aaattcgtca cacttaagtg
      181 gctatttttc aacttgacca gggtctaaga tatctgccgc gcggccctca cgcatgtaga
      241 atctcaggtc aagggcgatg tttacgtggt atcctcttga tcagcggaaa atcaccaaga
      301 atccggccta catgcctcgg agcgccatta gaaacgtagc cgttgcgttc gtaaaacgtc
      361 cttgcgggta tactgctcaa tacgcggatg tggtcaattc cagaagctat cgcttgagcc
      421 tctaacgctt taagcattgc cttgccaata ccctgatgca acgcctcggg aagaacatag
      481 ttgagcatga tctcggcacc gtacatgctc gaaaaaccca aaacttgacc gtgaagattg
      541 accgccacga cgcaatgaga cccgtcagag tcgatccact gccggacgtt ggcaggggtt
      601 ttattctcaa gccattgcgc caaaatctct tcatcgttct cgtagtaggg cgcacagatt
      661 tccttgatcg agcggaccag taccgcgcat gctgcttcgg cgtcttcggg agccgccttt
      721 cggatggtaa tatcgtccac gttaactacg agaccgacct tgtcttagcg ccttagggcc
      781 tgcgctggaa cgggattcag gcattctact caagaatatc ccgaatgaca cgacaagggt
      841 tgcctgccgc aaaaaccccg gccgggatat cgcgcgtcac tacgctgcca gcgccgatta
      901 ccgcgcgcga cccgacatga acaccagcta agatcagcgc gccaccacca acccaaacgt
      961 ctgctccgat ctctatcggt ttcccagatt cctgccctct gcgcaaaacg gcgttgaacg
     1021 gatggacggg cgtcaggatc tgtaccccag gaccgaacag agtgtagtct ccgattcgca
     1081 cttcgcaaac gtccagtact atacagttga agttaaagaa tatccgcgtt ccaagataga
     1141 tgttcgagcc gtagtcgcaa tagaatggcg attgcatcca gacggtatca ccgccggcgc
     1201 cgaacaaatc acaaagcagg cttcgtcgga gttcccgttc tgactcgcgt gtttgattga
     1261 ggccctggca cagatctcgt gcgcgttcac gcgccgcgac gagatccgga tcgagcgggt
     1321 cgtatagctc gcctgcgagc atcttgtcac gttcagtcat ccggatccgt cactgatgag
     1381 cagtaacatt tcgcgatcgg caggaaccta ggtttgactc tctcattcat agcgcgctat
     1441 cgctttcgcg tgaatatctg tgatcggcct gaaaacttcc cgtgagttgc tgcatccact
     1501 aaatcggtgc tcgatagccg tactcgcagg atccttgccc gggagagaat tacctgtcgg
     1561 gcctcaaacg ctaccagtat acgcccgctt tgatttctcg atgtcgatct ttttcattag
     1621 cagcatcgct tccataactt ttacggattt ttcggaatcc ggatcattga tcatctcgat
     1681 caaaactcgc ggaacgattt gccacgacac cccgtattta tccttgagcc agccacacat
     1741 ctgagttttt tcatccccgc ccaccaacgc cgacgaggag ggcgccggcg aaaaggaaat
     1801 cggccacatc ccagttcact tcgtcggtga actgcatcgc aatcaagggc agcagcagta
     1861 ccagcgcggc ggcggcccag ggcgctattc tccaaccgcc cacgcgccgt ccgccaccgt
     1921 tttccgtctt tcctgttatt gtcttgcctc cgtgcatgtc gttgctcatc gttcttccga
     1981 ggttgatgga gagggggtcg cactgcaata tactttacta cgcaaagtaa aatataaagt
     2041 tctctgcccc caaatccctt tcagaaactc cttgtggagc gcataatttg gcttgcgttt
     2101 ttcattcagg cacaatctcc acaaccgctg gggtggtcga ttcgagccgc acgaaacccg
     2161 catgtacgcg attcattcgg ctgagatagt tggccggatc tgcttcgaca atttgcaaac
     2221 gggcgtcgtg ccacgcgcct cgtaagtgaa tcctgaggcg accgtcgttt gcgagcgcgt
     2281 tgcggaccca atcggcgcgc gggccgtcct ccacgacgac gatgatgcgc ccgccatcgt
     2341 cgagataccc tacggggata cggcggcgct tacccgagcg acggccggcg gtttcgacga
     2401 tgagcgcgtt ttctcgggtg tatggaggcc gtgggatacc aaccaaaagg gcaaggttag
     2461 tgatctcatt tgcgatacga acgacccatt tcggaaggcg catatgattg cctctagcag
     2521 cgaaaagtta tgcgctgagc cgctaacaac aaaacagaag attcgtcatc gtatcgccgt
     2581 cgcctcaagc ggcggtttag gcatcggctg ccgttactgg tcgggcctca ggatcgactc
     2641 aatttgcctc gcaggtgcca tacactcggc gggcttcagc gataaatcga atgcctggtt
     2701 tagaatatcg ctaactgctt cctcgggcgt ggcgaatctc ggaaacaccc taatggccat
     2761 aataatgtcg atcgcttcaa tgcgataccc atcaagatca gcaatcctgt cggcgcctga
     2821 cccgagcgga ttccactcag taaggacagc cgctaccttt gaaatttgcg cctcatccat
     2881 agtttcgaac ctataccctc aactttggtg cggctaaacc gcgaccgata tatttagtat
     2941 tggttccgga attgcccgcg aaaccgctca ggttattctt tgccccaacc gctgacgacc
     3001 tccggccgta tccggaactg cacccgtcgc tctcccgggg cgcgataggg gtattcctcg
     3061 acgccgagat acttcttggc aaggcggttg atccctgcgc cggccccccg gtcttcagtg
     3121 atgacctcga ccacgcggcc gcgcaggccg aggttgcggt aggggttttc aggatcaagg
     3181 acactgagcg cgacggggct cccgaggtgc aggacgttgg ccttggtccg gccctccgct
     3241 gtgttgagaa taatatcctc gccgtccaga tccacccaca tcgccgatac gtgaggtgtc
     3301 ccgtccggca aaacgatgcc aacgtgaacg agcacctgct tctcgagaat ctgacgctcc
     3361 ttcgcggtca actgccgatg cgctactgtc atgtttggat gctgcagagc catctttcct
     3421 cctttgtcct atctaacacc ttggctcgcc gtgacgaata agcgagcctc aaaggcgagc
     3481 gttctcgaca tcaatcttca gccgcatgtt agacggcgat tctcccgggt accgcttccc
     3541 atcggttaga ttttttccct cgagcggcga tcataaaacc gattgccgcc gcacacgcgc
     3601 cgaagaatcc gatcgatact atagcaatca actacggtcg tgggtcaagt gccaggatga
     3661 cgacgcagcg gccgcgaatg cgacagagcc aacatcacgg catcatacat tggcaaagtg
     3721 ttgtcgtcat agtaaatccg tgttccttct gaatcagcga atgccataag tcactccagt
     3781 aggtaagcag cctgacggcg aagcatcagc cgcggcccga gcgtgcggcg cggggaccgg
     3841 ccgctggagc ctatttgttg ggcggcagtt gctcgcttac attacactcg cgagtgttca
     3901 ggaaagcgca cgcaccgctc gaagcgcgtg ctctacctgc tcctcgtcat tgaccagact
     3961 cggcgcgagg cgcgcatagg aaaccttgta cggagacctc gaagcgatca cttttttgtc
     4021 caagagtttg ggcactgcct catcgggggc catgccagcg atctcgaagc agatgattcc
     4081 ggcagagagt tccggcgagt ggggtgtgtg cagcttgacg agcggaatgc ctgcaaggcc
     4141 ctccttgcac tgtgtattta gggcggcgat gcggtcggca atgcgcttgc ggcctatgga
     4201 ccggtgaaac gcgaaagcct ccggcatggc ccactggtgc tcgtaggcct tgaatccgcc
     4261 cggcgagaca aagctcgcct gcgtcggccc ggggtccttg ccctccatcc aggcgtgcac
     4321 ggcgaggccg tgaaaggcag gaacggtcgg gcggatgcgg gcccactggt cgcgacggcc
     4381 ccagacaatt ccggtacccc ggggcgcaaa gatccacttg tgagtccctg cgacgaagaa
     4441 atcgcagccc atgtccaccg gcgactcatc ctggcttcca aaaccgtgca cgccgtccac
     4501 caccagcagg atgcggtcct tctccccgcg cttgccattg gcctcggaca cgaccgctgc
     4561 gagctgacga atcggaagct tgacgcccgt tgacgagtga acccaggtca gcccaaccac
     4621 gcgtgtcttg cgtccgattg cacgccgcag gttggtaacc acttcatcca tggtgaccgc
     4681 gccgagcttc tgcgtcgcga gccggatcgc ctcgtgatgc acgtaatggt catgcgtcgt
     4741 ggtcaggatt tcgtcatcgc gcttcaaagg catgccgttg tagatgaggg ccagcccttc
     4801 cgttgtgctg tccactagag cgatttcatc gggcttggcg cccatgtagt tggcggcggc
     4861 ctgct
//
LOCUS       DASUVS010000188         4406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_949154, whole genome shotgun sequence.
ACCESSION   DASUVS010000188 DASUVS010000000
VERSION     DASUVS010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949154"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VF182_10525"
     CDS             <1..730
                     /locus_tag="VF182_10525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6437556.1"
                     /translation="TELMTLLLTHPHFFSVVAELDGKIVGSNFLDERSAIAGVGPITV
                     EPSIQNRGVGRRLMEAVLSRASEKAHPGVRLLQSAYHGRSLALYTTLGFQVREGIACM
                     QGSPIGASLASYPVRPAREEDLEACDRICRFVHGHDRHRELLDAVKLGTAKLVAHDGR
                     ITGYATDFGFFGHAAAETTRDLIALISSASAFTGPGILVPMRNAPLFQWCLSQRLKIV
                     QVLTLMTRGLYNEPVGAYLPTILY"
     gene            complement(846..1208)
                     /locus_tag="VF182_10530"
     CDS             complement(846..1208)
                     /locus_tag="VF182_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437557.1"
                     /translation="MIRFQIFERTGVNLYKTLVDAMRAGELRTFEATKRGRRIQHKNV
                     LYPGWMNWSAHDGMINCEVISPRKPGREWQFFSAFLGRLADRYADQIIGINIQFPATH
                     SESGKAPVKKSRRMGRRK"
     gene            1395..2402
                     /locus_tag="VF182_10535"
     CDS             1395..2402
                     /locus_tag="VF182_10535"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437558.1"
                     /translation="MSVFSARRLILILVLALHFSASAILQAQTLKVPYVSLSPTAGPL
                     WIAYEAGYFRKNNIASELLYIPGGSTIIQAIISGDVKVANMAPPSAIGAWAKGFDLTL
                     IASGVDQLLETVVTGQTIRTVADLKNKKIGVSRFGSLTDMALREALRLHKLNPDKDVT
                     ILQTGGEATRFAALTSGAIDGAMLSGDKKVQAEKMGFHVAIDLSQLPIYYPVNGVVAS
                     KKFVSSNPEVVENFLKGWLEGIKTFKTDKELSLKVLAKYLKINDRAILEESYEIYRPV
                     YKKLPYGDKRAVKFALDQMGKELPDASKLNAEDFIYNGILTKLEKSGFIDQIYGESAK
                     R"
     gene            2415..3215
                     /locus_tag="VF182_10540"
     CDS             2415..3215
                     /locus_tag="VF182_10540"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6437559.1"
                     /translation="MAAKIKHLAIISDNYALLSRFYEALFGMKTSKSPRPESAVAIGD
                     GYVGMNIIPQKLGRQAGLEHFGLEVEDVEKIAGRLREKYPSVQLIKRPINRPFAGVST
                     HDPEGYVFDLSQQDMTNRGEVYVEGEWKQDRYVSHFVLRALNPANLAMFYREIYELQE
                     MEKPADDPSHYLTDGRMTMIISPWAITDFEGAGIEPRHMDHLGFRVEDVESFKKDLKE
                     LVNRNPALAPKPGGTEGDIRLKLLAKCKHGQFQLADPDGVPIDVASGR"
BASE COUNT         1042 a         1160 c         1161 g         1043 t
ORIGIN      
        1 taccgaactc atgaccctat tgctcacgca tccgcatttc ttcagtgtcg tggcagagct
       61 cgacggcaag atcgtcggca gcaattttct tgacgagcgc tctgcaatcg ctggggtagg
      121 cccgattacc gtagagccaa gcattcagaa ccgcggggtc gggcgccggc tgatggaagc
      181 cgttctcagc cgcgccagcg aaaaagccca tccaggcgtg cgacttcttc agtccgccta
      241 tcacggtcgc tccctggctt tatatacgac gttaggtttt caagtgcgcg aaggaattgc
      301 ttgcatgcag ggctccccga taggcgcctc gctggcgagc tatcctgtac gcccggcgag
      361 agaggaagat ctcgaagctt gtgatcgcat ttgccggttc gtccatggac acgaccgtca
      421 tcgagagctg ctggacgccg ttaagcttgg cacagcgaag ctcgtcgcgc acgatggacg
      481 aatcaccggt tatgccactg attttggttt ctttggtcat gccgcggctg aaaccacccg
      541 cgacctgatc gcgctgatta gttcggcttc tgcttttacc ggtccgggga ttctcgttcc
      601 gatgcgcaac gcgccgcttt ttcaatggtg tttgagtcag cgcttgaaga ttgtccaagt
      661 actaactcta atgacccgcg ggctttacaa cgaaccggta ggggcctacc tgccgacgat
      721 cctctattaa cgaatagcaa aaccggagag tagtcgagag ccattgcgca actcgagact
      781 cgctatgagg aagtctcgcc gcaaaaaaga ctgatcgctc gatgattacg gcgctcgtgg
      841 ttaagttatt tgcgccggcc catgcgccgg ctctttttaa caggtgcttt gccggattct
      901 gaatgtgtgg cggggaattg aatattgatg ccgatgatct ggtcggcgta acgatctgcc
      961 aggcgaccaa gaaaagcgct gaagaattgc cactcgcgac ccggcttccg aggacttatg
     1021 acttcgcaat tgatcatgcc gtcatgggca gaccagttca tccacccagg atagagtacg
     1081 ttcttatgct ggatccgtcg cccacgcttg gttgcttcga aggtgcgcag ttcgcccgca
     1141 cgcatcgcat ccaccagtgt cttgtaaaga ttcactccgg tccgttcgaa aatctgaaag
     1201 cgaatcatgc tcggcacctc ccgttgtgtc ggttacctgc ctggcgcaga attttacggg
     1261 tagccactct ggttggcaag cgtttcgttt ttgggcctcg atgaaaccgt tgacacgaga
     1321 acgacttaat tgttaaggca agcagtgatc cggtagtgca caatgatggc gagttaatcg
     1381 gtcaaaggac agctatgagt gttttttctg cgcgtagatt gattctcatc ttggttcttg
     1441 cactccattt ctcggcatcg gcaatcctgc aggctcagac acttaaggtt ccatatgttt
     1501 cgctctcccc caccgccggt ccgttgtgga tcgcgtacga agccggctac tttagaaaaa
     1561 acaacatcgc cagcgagcta ctctacatcc ccggcggatc gacgatcatt caggcgatca
     1621 tctcgggcga cgtcaaagtg gccaacatgg cgcctccgtc agccatcggc gcctgggcca
     1681 aaggcttcga tctgacgttg atcgccagcg gggtcgatca actgctggag acggtggtca
     1741 ccggacagac catcagaacc gtggctgatc tcaagaataa gaagatcggc gtcagccgct
     1801 tcggttctct cacggatatg gcgcttcgcg aggccttgcg gctgcacaag ctcaacccgg
     1861 ataaagatgt aaccatttta caaaccggag gcgaggcgac gcgctttgcc gcccttacca
     1921 gcggcgctat cgatggagcg atgctttcgg gcgacaagaa agttcaggcg gaaaagatgg
     1981 gctttcatgt ggccatcgat ctcagtcagt tgccaatcta ttatccggtc aacggcgtgg
     2041 tcgcgtccaa aaagtttgtc tcgagtaatc ccgaagttgt tgaaaacttt ctcaaaggct
     2101 ggctagaagg aatcaaaact ttcaagaccg acaaagaact atcgctcaaa gtcctcgcca
     2161 aatatctaaa gattaacgac cgagcgattc tggaggagag ttatgaaata taccggccgg
     2221 tttacaagaa gctgccgtat ggcgataaga gagcggtgaa gttcgcactc gaccagatgg
     2281 gcaaagaact ccccgatgcc agcaaactca acgctgaaga tttcatttac aacgggatcc
     2341 tcacaaaatt ggagaagagc ggttttatcg atcaaatcta cggcgaatct gcaaaacgat
     2401 aaaaggaatt aaacatggcc gcgaaaatca aacatttagc gatcatcagc gacaactacg
     2461 ctttattgag ccggttctac gaagcgctat tcggcatgaa aacttcaaaa tcaccgcggc
     2521 cggaaagcgc cgtggcgatc ggcgacggtt atgtcggcat gaacatcatc ccgcagaagc
     2581 tgggacggca agcgggcctg gaacattttg gattggaagt cgaagacgtc gaaaaaatag
     2641 cgggccgctt gcgggagaag tatccgtccg ttcagctgat caagagaccg atcaatcgcc
     2701 ccttcgccgg cgtcagcacc cacgatccgg aaggctatgt cttcgactta tcgcaacagg
     2761 atatgaccaa tcggggcgaa gtctatgttg aaggcgaatg gaagcaggat cgatacgtca
     2821 gccacttcgt cctgcgcgcg cttaacccgg ccaatctcgc gatgttctat cgcgaaatct
     2881 atgagcttca agaaatggaa aagcccgccg acgatcccag ccattatctt accgacggcc
     2941 gcatgacgat gatcatctca ccctgggcta ttacggattt tgaaggcgcc ggcatcgagc
     3001 cacggcatat ggatcacctg ggattcagag ttgaggatgt cgaatcattc aaaaaggatc
     3061 taaaagaact ggtcaataga aaccctgccc ttgcgcccaa acccggcggc accgaaggcg
     3121 atatccgtct caagctgctc gccaagtgca agcacggaca gtttcagctt gccgatccgg
     3181 acggcgtgcc catcgacgtg gcatctgggc gttaaggatt cccaatagca aaatcgccgg
     3241 tcaaggaatg ttgtctcccg agctgactcg gatccactct atcccaagag gattacgaga
     3301 attaataccg accttttggt gctccctctg tgaatttgga ttgacagttt gcgaccataa
     3361 atgcctaacg attttgatcc tagagaacga gcagtggagg ccactcggct tctcaattcc
     3421 tttctcgtag attttattgt tggaacccgc ggacttgaga tctttgattc tccggccata
     3481 gcaccgaagg tgacacagag gattgcgatc ggcttgaacc gaatggcaat ttcccatcta
     3541 agtatttgtc tttcgaagtg gacagagttt tacaggtctt gccgtgcgat tctgccgctg
     3601 gatgtccgta gtgcatgtct gaaggtgcat aatgaagtca tctctcgcgg tattgtcgat
     3661 tttcggaaca aggttattgg ccatatagca gatgacgata cgaagcggcc ttggacggcc
     3721 gctgagattg atgaacattt taaccgaatg atcgccggta atcgtgagga tttcttgcgc
     3781 tggatcaata atccagaagc caacatcttc ccacactcgg ttgtcgcgat tacggagcac
     3841 gtgcgggata gactccgaac agactatgga gttcgcgatg acgagatcgg atgagtgttt
     3901 aacgttgttg ttagttcagg cgacccacta acacccgtgg agcggacatc ggaccattac
     3961 tatcactcaa atccggctgc tagattcagc atgaatctcc tgccttctgg taatccgctc
     4021 gtttgccttt gccacaccct actttcgggg ttcgcccctt acgaaagttt ccgtgtccgc
     4081 agctttcagt aacgccttca gtgagtcagg atctgcggcc tgcacatagc ccacggttac
     4141 gatgccgtca accgtttacc cttgacggtt tagcgtagtt tcgattagcc tcgggccaac
     4201 tggacgttgg tcgtaaacca atcgcagctt ccaacctgtg ggggtgcaac atgatgacgg
     4261 ttttgatgat tcttctgggt tttttgtttt tcggcatgaa cgcacaggca cagacgcctt
     4321 tctatcaagg gaaaaccatc accgtgatca tcggcacgcc gccgggaaat ctttatgatc
     4381 tctgggggcg tttgattgtc gaacac
//
LOCUS       DASUVS010000189         2551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_949275, whole genome shotgun sequence.
ACCESSION   DASUVS010000189 DASUVS010000000
VERSION     DASUVS010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949275"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VF182_10545"
     CDS             complement(<1..801)
                     /locus_tag="VF182_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6437560.1"
                     /translation="MKAVDHLNNKTRNIIGTPTQRLEGPEKVSGQAIYATDVVVPGML
                     WCKVLRSPISYGRIQRIDASRALALSGVRAVATGEDVKGFLIGRKIYDMPILADGVVR
                     FIGEKVAAVAAESEAIAEAAVELIDVEYEEMEPLLDPLDAIEPDAPLLHPNVTGYRGL
                     LHSIETPSNVFVDMNWEKGDVEAGFRESDIVVENVFTTRAVHQAYIEPHACVVQAQEQ
                     GHVDIWACSKVPFALREQVATAFGKGLEQFTVHPCYIGGCFGGKGDFMD"
     gene            complement(798..1559)
                     /locus_tag="VF182_10550"
     CDS             complement(798..1559)
                     /locus_tag="VF182_10550"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6437561.1"
                     /translation="MTTSMDSRQAKALVDEHYRKVAKRWQERVIDGPFMKQLQAGKLP
                     ANALKTFFKNWGSYTIEINTLEAASYHKHISFFRKHRDLMGPMAAKLADELIHPKPPG
                     HVHVVVETAKALGITEDEIFLAPMLAEFRAKLDFKRSILWEGTVAEFYAAGATEEQTG
                     YWSAEFFKALTAHYGLTREQAVYFSTHEEADLEEHDGVMGHGSFRRLVLQRLFEDGMA
                     TARPGYTLEYCGLTAVDLHGVCLQAALDCAGRESQ"
     gene            complement(1577..2176)
                     /locus_tag="VF182_10555"
     CDS             complement(1577..2176)
                     /locus_tag="VF182_10555"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6437562.1"
                     /translation="MIRFDCLELLAPLITDHIVVTSLSGQKIEWAHLSKHDGNLLVGT
                     MGTALGVGMGLAVALPQRKVIVLESDGSVLLSLFNLPTLANLDLNNLIVFVFDNASYS
                     GSRISEPTATAGKTDLAKMAQAAGIENAVTVRDLQSFKQEMTAAMNESGVRFIVCKVE
                     ESTEHRNIPRTNMDPLENKYVFVRYLERTEGKKIVRSHG"
     gene            complement(2173..>2551)
                     /locus_tag="VF182_10560"
     CDS             complement(2173..>2551)
                     /locus_tag="VF182_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437563.1"
                     /translation="LIPVSSEAEAITIGAGAALGGKQVAVYMECTGLYVSCYSILSIA
                     KRFGVPLLLLIAHLGSFADQRNNSVYAVSGTYVTPVLDALEIQYRLVESGDNLETCIK
                     DAARHTNALKLPVALLFSGEFTR"
BASE COUNT          544 a          757 c          678 g          572 t
ORIGIN      
        1 atccatgaaa tcgcctttgc caccgaagca accgccgata taacaaggat gaaccgtgaa
       61 ctgctcaagc cctttgccaa acgccgtggc gacttgctcg cgcagcgcga atggcacttt
      121 ggagcatgcc caaatatcca catggccctg ttcctgtgct tggacgacgc aagcgtgcgg
      181 ctcgatataa gcctgatgga ccgctctggt ggtaaaaaca ttttcgacga cgatgtcgga
      241 ttcgcggaac cccgcttcga catcgccttt ctcccaattc atatcgacaa aaacgttgct
      301 tggcgtttca attgaatgaa gcaagccgcg atagccggtc acgttgggat gaagcagcgg
      361 cgcgtccggt tcaatcgcat cgagcgggtc gagcagcggt tccatttctt catattcaac
      421 atcgatcaat tccactgccg cttcggcaat cgcctcgctt tcggcggcca ccgccgccac
      481 tttctcgccg ataaacctga caacgccatc ggcgaggatc ggcatgtcgt aaattttccg
      541 gccgatcaga aaacctttga cgtcttcgcc ggtggcaacc gcgcgcaccc ccgacaaggc
      601 caatgcgcga ctggcatcga ttctctggat ccggccgtag gaaatcgggc tgcgcagaac
      661 tttacaccag agcattcccg gcaccaccac gtcagtggca taaattgcct ggcccgacac
      721 tttttccgga ccttccagac gttgcgtggg agtgccgatg atattgcggg ttttattatt
      781 tagatgatcg actgctttca ctgtgattcc ctgccggcgc aatccagagc ggcttgcagg
      841 cagacgccgt gcagatcgac ggccgtcaac ccgcaatatt cgagagtgta gccgggccgc
      901 gcggtcgcca tgccgtcttc gaagagccgc tgcaaaacca gccgtctaaa actgccgtga
      961 cccatcacgc catcgtgctc ctcgaggtcg gcctcttcat gggtcgaaaa atagaccgcc
     1021 tgctcgcggg tcagcccgta atgcgcggtc agtgctttaa agaattccgc cgaccagtag
     1081 cccgtctgct cttcggttgc acccgcggca tagaactcgg ccaccgtccc ttcccagagg
     1141 atcgaccgtt tgaaatcgag cttggcgcga aactcggcca gcatcggcgc gagaaaaatc
     1201 tcatcctcag taatccccag cgccttggcg gtttcgacta cgacgtggac gtgaccgggt
     1261 ggtttagggt gaatcaattc atccgccagc tttgccgcca ttggccccat gagatcgcga
     1321 tgcttgcgga aaaaagagat gtgcttgtga taggaggccg cttccagggt gttgatctcg
     1381 atggtatacg agccccagtt cttaaaaaac gtttttaaag cgtttgccgg cagctttccc
     1441 gcttgcaact gcttcataaa gggcccgtcg atcactctct cctgccagcg cttggcgact
     1501 ttgcggtagt gctcatcgac caacgctttg gcttgccggc tatccatcga ggtcgtcatc
     1561 tgattctcct gtttcgttaa ccgtgactgc ggacgatctt tttcccttcg gtcctttcga
     1621 gataccgaac gaatacatac ttgttctcca gcggatccat attcgttcgc gggatgttgc
     1681 gatgctccgt gctttcctcc accttgcaca cgataaatct caccccgctc tcgttcatcg
     1741 ccgcggtcat ctcctgtttg aacgattgaa ggtcgcgcac agtcacagca ttttcaatac
     1801 cggcggcttg cgccatcttg gccagatcgg tttttcccgc ggtggcggtc ggttcgctga
     1861 tccgactgcc gctgtaggaa gcgttgtcga aaacgaaaac aatcaagttg tttaaatcga
     1921 gattggccag ggtcggcaga ttgaacaagg acaagagaac gctgccgtcc gattcgagca
     1981 cgattacttt tcgctgcggc agagcgacag cgagacccat gccgacaccg agcgccgtgc
     2041 ccatggtccc gaccagcaga ttgccgtcat gcttggacaa gtgtgcccac tcgattttct
     2101 ggccgcttaa cgaagtcacg acgatgtgat cggtaatcag cggcgcgagg agctcaagac
     2161 agtcgaatcg gatcatcggg taaattctcc gctaaacaga agagccaccg gcagcttgag
     2221 cgcgttggtg tggcgcgccg cgtctttgat gcaggtttcc agattgtcgc cgctttcaac
     2281 cagccggtac tgaatctcca gggcgtcgag caccggcgtc acatacgttc ccgaaaccgc
     2341 atagacggaa ttgttgcgct gatcggcaaa actgccgaga tgagcgatca gcagcaggag
     2401 cggcactccg aaacgttttg cgatcgataa gatactgtag caggaaacat agagcccggt
     2461 gcattccatg taaaccgcaa cttgcttgcc gcccaaagcc gcgcccgcgc cgatggtgat
     2521 cgcctcggct tcgctggaaa ccggaatgag c
//
LOCUS       DASUVS010000190        23185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1388215, whole genome shotgun sequence.
ACCESSION   DASUVS010000190 DASUVS010000000
VERSION     DASUVS010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 23185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23185
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388215"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VF182_10565"
     CDS             <1..213
                     /locus_tag="VF182_10565"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007637873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HEX6437564.1"
                     /translation="GTGWPSFFTTIPGVFGTRTDYRLILPRTEYHCIRCVGHHGHLFS
                     DGPKPTGQRWCNNGIALKFIPQSGSV"
     gene            complement(301..939)
                     /locus_tag="VF182_10570"
     CDS             complement(301..939)
                     /locus_tag="VF182_10570"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEX6437565.1"
                     /translation="MSSMKIFFFDIETIPTDQSVLDNNLLAAQMRLDEPEIIKKLSLS
                     AATARILCLAYAIEPEIDSPVEILHGTESEIISGFWQLAADCHLFVGHNIIDFDLRFI
                     YQRSIINRIRPSREISLARFRNDPVYDTMHEWSKWGREHVSLDLLARALGIPSPKESL
                     DGSKVYPYFCAGKLPDICEYCKRDVETVRQVYRRLTFAKISETMTPPLEIAE"
     gene            complement(940..4365)
                     /locus_tag="VF182_10575"
     CDS             complement(940..4365)
                     /locus_tag="VF182_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX6437566.1"
                     /translation="MATVLADARDRSTAVETFDRNLVVTAGAGTGKTTLLIDRIVHLL
                     LRNPDPLLITDIVALTFTNKAANEMKQRLRDRLQAYLKVDLRAEPPGEDREKLQLQVA
                     SLISSYRLSKDELDARVQEALRNLERAEIGTIHSFAATLLRLFPLEAGVDPQFKEDDG
                     KQFRRLFDQQWHLWLDQELALESPRAGEWRAALKHLTLEQIKDLARSLCSESVQLEPL
                     RANQPANAALGAWLRSLLHSAASLSKRHPEDRNNEKLVRAAHSIIQAFIDGADANGTA
                     LGADKACLREHAINRETKGWSEADIKQAHSIVTAAKALCRVDAGLTHLIRHLLLPYAA
                     RFRETFVHEGFISFDGLLIRARDLVRDHLRVREELKGQYRTFLIDEFQDTDPIQYEIL
                     LYLTEQPGSTAADWRKVKLTAGKVFVVGDPKQSIYAFRRADIEAYLEVVEKIIKAQNG
                     TECHLLTNFRSDTAIIDVVNGVFSSLIQPKPGMQPPYIGIQPPPGHDSPAGIGSAKPL
                     PKAMMRKIVAPDGEVSAETARRLEGESLADWLHDQVLDEAGFVNARGEWVLAQPKDVA
                     ILFRKLTDIHDYLEPLRRRGIRYVVEGERHFYAAKEIIDAVNLLRAIENPNDRLALVG
                     VLRSPIGGLSDQNIYDLHCDNLLDYRRSEQLSGKNFPPALTELYRALAKLHAECRILP
                     VGAAVSQIFDRLPLRLLAACYFHGEQAVANIDKLRRQAELLGREDSSMTFKAAIRQLQ
                     QRVLDVEEEGESVLAEEDVDAVRIMSVHKSKGLEFPIVVLAGCHAGINGQQRRPAEAL
                     FDWSSGLTGIRVGSFTDLAGVYIAEKNKLRAEEETKRLLYVAMTRAREHLIISSGPAA
                     RKFTGNFVGMLDEAAQDQIGCAEQSTTIEIGCGRLAVELVEASLTAPGAATSKKKPLD
                     KKRDWQVYIEAWGHREEKYQAALQTATFVTPTSLKRREQEITEASVTTQAFAQERAPA
                     MLVGALAHRFLEHWDFGGDRNLLRDQLGAFLSASLPAEYSCRASSIGKELQATLEPFV
                     SSDIYAELSKARILGREVPLLMSWKEQIMEGVIDVIYERKGLLYLADYKTDRIEAKNL
                     RLGAERYRQQAEIYTEAARRSLRREVAAFKLIFLRLGESVEIDFSPEKEPWLF"
     gene            complement(4377..7655)
                     /locus_tag="VF182_10580"
     CDS             complement(4377..7655)
                     /locus_tag="VF182_10580"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX6437567.1"
                     /translation="MMQALLAPFHPHLENSLVEEVLHYKNTDPLCPLLILVPSDALRR
                     HLKILLIRERNLSFINLQLLTFHQLSAKLFAEAKGLNPPVLRDDLFLEETLRQIIRAQ
                     QPGTAAFSGIEERAGGCAALWQSLRDLRDGLVQPDIALEAFRENQFDRRTHETTANLL
                     VMLQTFLRFCQEHGILDHSTLDKSAIATAPASDFLGQFARIFYYGFYDLTQIQVDLVH
                     AVARAVPTTLFFPLLETQPAHEGWQFAKRFYAVYIQGRADNPSTNNPPAECDHRIDLP
                     QIAAVFDQDPHRQYQPLPANWHCRITNTFGIDDEISAVAKEILLLTEQDGMAFDQIGV
                     VARTLEGYGAKIQEVFAQHRIPITGSIDESIAQFPLTKAAILLMNLAARDYLRSHVID
                     LVSSPYFRLSGAAQPEIELRPDLWDLATRELAICKGMKEWQRIDRYVRVGMKLSQISD
                     DEERRFIEIPATQLRALATILKGLDNVLSRLPARASWTDYADSWKQMFGAYLGIQPEP
                     SGGFNITETLICKKIIEILDQISGFDAVEAEVSLREFSETLQHWLERSTIVETNKNLA
                     GVTVTSASAARGIKFRALFIVGMNEGVFPRPIREDAFLRDRDREVLERDLGYKVGQKL
                     AAFDEEKLVFTLLVNSATERLYCSYQRSDESGRVLAPSWYLAELKAALASAQQEQFVE
                     QSIPRSTVDKVRSRPFDVEHSLLPAELAIRLNLSGTDSRSLIAAASLSPSLYEQGLKA
                     IAAIDLSTDRLDVFDGIIQTPAPHWQRFSKRGLSPTALELYGRCPFQYFARQLLGLQS
                     FEAPETAMGPNAAEMGELGHLILKLTYQELIQRGYFADRGSPVAVDSIISAAAEKVFT
                     QYEAENPIGYPLMWETMRETLTQLIREVVGRDLQELSSSGYVPVDLEVGIDDRLPPDW
                     PAPLNTLPIHGRIDRIDSDPLGNRLRVVDYKFKFGGKPSPEDNDLTRAALRGQRLQPP
                     FYSLLGKSKRRRDSLRNGEYQIEASFYYIALRWSDGPLVIKSFNSEELSGRIGEEIKN
                     SMAQLGKGIQAGQFFMQPGEYCPHCEIAEICRKNHPPSLWRAENDPITAPHRQLRQKD
                     IKKC"
     gene            7837..9516
                     /gene="ggt"
                     /locus_tag="VF182_10585"
     CDS             7837..9516
                     /gene="ggt"
                     /locus_tag="VF182_10585"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX6437568.1"
                     /translation="MRCLIPLRFVFIFIFLSNTFFAPSLVLAQRATGLVVSDSPLATQ
                     AGMEMLGRGGNAIDAAIATAYALAVVDQASSGIGGGGFMVIYETKDRRAHALDFREAA
                     PEGTRKDLYIKDGKPVPSLSLTGALAVAVPGEVAGLAQAHKRFGSMPMQVLMAPAIRL
                     ASNGFPLDATLRYAIDRQQGSMKKFADLGRIYMPNNEVPAEGELIRQPELGDTLKAIA
                     SQGADAFYQGWIADAIVETIKREGGVLNSEDLKNYRPVWREPLIGNYRQRTVIGMPPP
                     SSGGVAILQMLKVLEGYHLSALPHNSATYLHLITETMKHAFADRAQYLGDPDFVKPPV
                     PLLTSKDYARWIRGRISAAKTQPTRYYGLVNFKPELGGTTHFSVMDRFGNAVACTISV
                     NTRFGSKLLVPDTGIVLNNEMDDFAIHSTGNVYGLVGNEANSVLPRKRPLSSMSPTII
                     LQGDRPELVVGGAGGPRIISATLQTILNVLDYRMPVAKAVEAARVHHQWLPDQLSVEN
                     DIPIETRRSLERRGHAVRERGVLGVVQAITAGQDGVKGAADPRKEERARSE"
     gene            complement(9676..9981)
                     /locus_tag="VF182_10590"
     CDS             complement(9676..9981)
                     /locus_tag="VF182_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HEX6437569.1"
                     /translation="MNGETSRERLINDLKTMVSDAEELLRATTSQAGEKVAAARQKIE
                     QSLIEGKKALADAEQALFETSKEYADIADDYIRENPWNAVAIAAGIGLLVGLTIRRS"
     gene            complement(10209..11504)
                     /locus_tag="VF182_10595"
     CDS             complement(10209..11504)
                     /locus_tag="VF182_10595"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX6437570.1"
                     /translation="MSNVAIIGAQWGDEGKGKVVDLFTCDADIIVRFQGGNNAGHTLV
                     VDGKKTVLHLVPSGALHPDKLCVIGNGVVVDPEILIDEIDALKAQGHLLDDSLLRISE
                     EGHLIMPYHKAIDLARERLRGKGKIGTTGRGIGPAYEDKVARVGIRFIDLLEEDTFRE
                     KLRHNIEEKNIYLKAILKEKTLDFNHIHDTYAGYRERLRPLIANTSLLLHREIRAGKK
                     VMFEGAQGTLLDVDHGTYPYVTSSNTSTGGACTGAGVGPQHIHHVIGISKAYTTRVGS
                     GPFITEQFGPEGEILKREGVEFGATTGRPRRCGWFDAVGVRHAVRVNGITGIALTKLD
                     VLTGFKKVPICTAYRFDGRLYDEFPASSKVLDKAEPVLEEMAGWNEPITGVRKFSELP
                     SAAQNYVQRIEKLLDTEIILVSVGPGREQTILLKNPFDS"
     gene            complement(11522..13105)
                     /gene="serA"
                     /locus_tag="VF182_10600"
     CDS             complement(11522..13105)
                     /gene="serA"
                     /locus_tag="VF182_10600"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX6437571.1"
                     /translation="MKILVSDSLAPQCLEVFKRAPGFEVDVRTGLDPNELKKICADYD
                     GWVIRSGTKISAELIEAAKNLKVIGRAGVGFENIDVDAATQRGIVVMNTPGGNNVTTG
                     EHTISLMMALARHIPQAVASLKNGKWERNKFIGVELCNKTIGVIGLGNVGRIVAERAA
                     GLRMKVLGYDPFISAENIARMEVEPAPLEEIFSKSDFITVHVPLTNETQGLINREAFA
                     KMKPGVRIINCARGGIVDEKDLAEAIKEGKVAGAALDVYVDEPPPADHPLLNMDQVIT
                     TPHLGASTDEAQLNVAIAVAEQMVDFLTEGLVRYAVNVPSVSPELFKILRPYLILAEK
                     LGSLQVQMADVLPQAVHVEYRGEVTQYNIAPLTLAVLKGMLTPVMESSVNYVNAPILA
                     KERGIQVVESKSSKAGDFSSSITVKIATGDREVEIEGAIFGDKHPRIVRVDNFYLEAA
                     PEGYILILRNKDVPGVVGNVGTILGKNGINIAGMELGRGAKGGNAISFIHVDDQIPKE
                     TMKELRNLPQTVSAELVKL"
     gene            complement(13108..14250)
                     /locus_tag="VF182_10605"
     CDS             complement(13108..14250)
                     /locus_tag="VF182_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX6437572.1"
                     /translation="MIKHYLLSPGPTPIPNEVALAMSETMIHHRTPQFNKVFDQARQG
                     LKKLFGTKSDVLMLASSGTGAMEASVANLFSPADKVLVINGGKFGERWLNIANAFGLN
                     PIELKVEWGQAVKVDAVDKQLKAHPDLKGVMVQASETSTTVLHPIREIAKLTKNGPLL
                     VVDGVTAVGVVSIPFDEWGLDVLVTGSQKAMMLPPGLGFIALSDRAWESTQQAKLPRF
                     YFDLNLERKNQQKGSGAFTPAVSLIFGARASLEMMQREGLDRVYARHARMSRATRAAA
                     TALGLKLLAPDNPSPAATGVYLPDGIDADQVLDYLRDHMNITLAEGQDQLKGKVIRIA
                     HVGYMGAFDVITAVAALEMVMRKFGVELPFGRGVAAAQEILMESLV"
     regulatory      complement(14416..14500)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            14606..15298
                     /locus_tag="VF182_10610"
     CDS             14606..15298
                     /locus_tag="VF182_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6437573.1"
                     /translation="MKRLLLFDIDGTLTRSHNGHIPFNQAILETFGVPGDIRSVIPDG
                     NTDPMIVTEIFSKSNILIDDIDDKWQQFSSNLRQCYSQAISSGNTSVRALPGTVELLQ
                     TVGVGGIFGSSVVSGNLEPMARVKLETAGLYSYLCRGAYGSDSPRRADLPAIAKHRCE
                     AAFGVSIPFNECIVIGDTPRDFEAARCNRMKCILVATGRYPLAELSKCQAERCLADLT
                     DTAAVLETLLNI"
     gene            15473..16468
                     /locus_tag="VF182_10615"
     CDS             15473..16468
                     /locus_tag="VF182_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6437574.1"
                     /translation="MQFRTIGKTGVRVSEIGFGCGNNAVLMVKASYEDQVKAVRHALD
                     LGINYFDTAFAYGLGKSEENLGKILNQLKTPAVISTKIRLEADALTDIKAATQREVEA
                     GLRRLQRDSVDLIQLHTRVVTGRQQDKRFGMTPGEILGRSGVIEGFKAMRDKGKVGYF
                     GFTGLGDPAALHEVVDSGAFHSFQAYYNLLNPSAGHPVPPAFSALDYGQLIDRAAARG
                     MGVAVIRVLAAGALTSNPGAGGGSTPEPLSKGSEYSLDVQRAEKLKAALAADGKGMTQ
                     AAIRFGLMNPKVSTVLVGFSNSAHIDEAVACSGAPPLSNEEMAMIQRLWQTDFGR"
     gene            16485..17600
                     /locus_tag="VF182_10620"
     CDS             16485..17600
                     /locus_tag="VF182_10620"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437575.1"
                     /translation="MALFFHSDEVDEFIPFTEAVAISENALRNIKTPAGVNAPRKRLN
                     LHREIAEGSFDTVLNIYAGGSAAYGAIGAQVALHRKAIAGNMQRRPPYNPDQTELALI
                     YDTDTGSLLGIMANRPRHVGGAADLRTPAVSLAGLEQFARRDARRVGIYGSGQQAYST
                     FVGLTEMRKIESAKIYSPTPAHREGFARKISQRTGVPVRAVENPELAARDVDIILCMT
                     NTNVPVIDGSWLEQGQYIISVVGSNIELVKSGNIAAPRREIDDETLNRCSFIVALSKE
                     QAIDSQQGDIYWPVQNGIMAWDKVIDISDILTGKAKGRTDDEQIILYKNQGGQGIVDI
                     ALAKKCFDLAKQHGKGHELRIEARENWWVQGGRTQTW"
     gene            17644..18777
                     /locus_tag="VF182_10625"
     CDS             17644..18777
                     /locus_tag="VF182_10625"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6437576.1"
                     /translation="MIGKSSGCTVQGFKRSKAQGSKSSTIANTLKFILRLAALIIFHL
                     TIPFTVRAVFAQTPYHQGKTITIVVGTVAGDLYDLYARAIGLVMGKYIPGNPNIIVQN
                     MPGAGHMIAANYIYNVAKPDGLTMAAINAGLYFEQLIGRPEAKFDWTQYTWIGNATKS
                     PQVLYMRADSPYKSIDDVRNAKEPPKCGTTGMSNMGYFVPKLLEETVGAKFNVVAGYQ
                     GGNEVDLGVERGEIQCRSLSSEAYFSREPFLSWRKRGFARELVQGGRSRDERMPAAPT
                     IYELMDKYNTPEPARRLATALLASGDFHRPYLAPPKLRPEHVKILREAFNKTMKDPEF
                     LAEAKKKRLDIDPTSGEEVEALTNEVMSQPPEVIERLKKLMGR"
     gene            complement(18893..19330)
                     /locus_tag="VF182_10630"
     CDS             complement(18893..19330)
                     /locus_tag="VF182_10630"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6437577.1"
                     /translation="MAAEFIDLLNLPELDIGHSKKVLFTSDHFHTWVHGDYPGTKGPM
                     HKHTADQQFYCVKGECTFHFPDTPARKLTPGMLVTIPAGQLYQLDNTGDEYMILLGAR
                     AEAAAKPRFSDKDEVVTTGDYAVQNLDKIKAPEDKKAKAQAKL"
     gene            complement(19454..>23185)
                     /locus_tag="VF182_10635"
     CDS             complement(19454..>23185)
                     /locus_tag="VF182_10635"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEX6437578.1"
                     /translation="LAATLLARFAQGVNVFDRTRNILIFIALAGVISTAAGATLGMTT
                     LYLGGFIAPPELSTVWLTWWLGDSVGNLIIAPLLLMWLTQPVLPVRSRHIFEAAGLLM
                     AVISMSALLFLNPVPAGLEYFALGPLLWAALRFGRRGAVTAAFVTSAIALWGTVRDLG
                     PFAPSEPHESIFLLQLFIATISMTALIVASVVSQQQRLEQRLRIKDAVSRIFSEASTM
                     AEAAPRVIKAICETAAWDFGALWLVNRNSNVLTCVDMWCLPSIDLSGFAAVTREITFE
                     RGTGLPGRVWESATPAWISDVLKDNNFPRAPVAISVGFHGAVGFPIKLDNEVFGVLEF
                     FSRETREPDDEFQEMVANLGTQFGQFIERQRAEAALRESEGRLQLALSAGQMGAWEWN
                     ISASKVTWSASLEVIHGLKRGSFGGAFEDLKKTIHPEDVESVLKELQKTLETRSDYEI
                     AYRIVHPDGQVRWVEALASLSYGADGHPEKLAGVCMDITESRHQEEMLRQRTRSLEII
                     NRVGYALAAELDLKKIAQIVIDAGREISGAQFGGFFYNVANKSGNSFPLYSLSGAPHN
                     VVDKLMPRNRQIFGSMFAGGVLRIGDVLNDPRFATTLPDQGSAEDALPARSYLSVPVS
                     SRSGDVIGGLLFGHSQPNLFTAEAESIVTAIAAQASVAFDNANLYQTVQRRVEEFQKL
                     IDTAPIGIAVATDSESKHIWGNPEFMRMVGTGADQNLSKTGAEQDQLSFKIFRNGKEL
                     PAEELPMQRAVREGVDVVDEELEIEREDGSIIHELCRATPLRDEHGKVRGCIGIFLNI
                     TDRKQGDAALQRARDDLARANEGLEKRIQLRTAELQMANAALFAEREEEKRLEQQLRQ
                     AQKMESMGTLASGIAHDFNNILNIIKGYASLLLQTTEDPQMAAEALQVIDETVERGAA
                     TVRQLLALARESNLQFEPIDLNEMLENLKSLLSGTLPKTIDIEVDLKNSLPQSMADPN
                     QLHQVFLNVCLNARDAMPHGGKLRLATDVIAGVELRKTYADAKEQSYACVTLEDTGSG
                     IDETVKHRIFEPFFTTKPQGQGSGLGLAVAYGIIANHRGFIEVTSEPGQGAAFRIYLP
                     LAETSQLNPESRISSRSEAPGSISTKGQLVLFVDDEKNQVRLMIAYLESAGYRVLTAL
                     DGVEAVETFRQYKDEISVVVLDLGLPKLNGWDVLKQMKIADPTIKPILASGYVSAEVE
                     SALDNGDLSAVIFKPYKLEEIKNAVAAAAVERRTLDVLG"
BASE COUNT         5078 a         6344 c         6425 g         5338 t
ORIGIN      
        1 ggcaccggct ggccgagttt cttcactacg attcccggcg tcttcggcac tcgcactgat
       61 tacaggttga tcttgccgcg caccgagtat cactgcatcc gttgcgtcgg gcatcatggc
      121 catctgttca gcgacggccc caagcccacc ggacaacgct ggtgcaacaa tggcattgcc
      181 ttgaaattca ttccgcagag cggcagcgtc taagcgtttc agttgggcag gcggatcatt
      241 cgccgccccg tgagagggcg cgcagctaac acagcatgaa tagcgcgccg cgagagattt
      301 ttattccgca atttcaagtg gcggggtcat agtttcgctg atcttggcaa acgtcaaacg
      361 gcggtacacc tgacgaacgg tttctacatc gcgtttgcag tactcgcaga tgtccggcag
      421 ctttcccgcg caaaaatacg ggtatacctt ggagccgtcc aggctttctt tgggggatgg
      481 gattccgagc gcccgagcga gaagatccag gctcacgtgt tcgcgccccc atttactcca
      541 ttcatgcatc gtgtcgtaaa ccggatcgtt acggaaccgg gccaacgaga tctcgcgcga
      601 aggtctgatc ctattgatga tggaacgctg gtagataaat cgcaggtcga agtcaatgat
      661 attgtggccc acgaacaagt gacagtccgc ggcaagctgc cagaagccgc tgataatctc
      721 cgactcggta ccgtgcagga tctcgacggg agaatcgatt tccggttcga tggcgtaagc
      781 caggcataga atacgcgcgg tggcggcgga aagactaagc ttcttgataa tctccggctc
      841 gtcgagacgc atttgcgcag ccagcaggtt gttgtcgagc accgactggt cggtgggaat
      901 ggtctcgata tcgaaaaaga aaattttcat ggatgacatt taaaaaagcc acggctcctt
      961 ttctggacta aaatcgattt cgacgctctc gcctaagcgc aaaaaaatta atttgaaagc
     1021 ggcgacttct cgtctcaagc tgcggcgcgc cgcttcggta tagatctcgg cttgttggcg
     1081 gtagcgctcc gcgccgagac gcagattctt tgcttcgatc cggtctgtct tatagtccgc
     1141 caggtacagc agtcctttgc gctcatagat gacatcgatg acgccttcca tgatctgctc
     1201 tttccaggac atcaacagcg gcacctcgcg gccgaggatc cgagccttgg acaattcagc
     1261 atagatatcc gaacttacga aaggctccag ggttgcttgc aactctttac ctatgctgct
     1321 tgcccggcaa ctatattccg ctggcagcga tgcgctaaga aaagcgccga gttgatcacg
     1381 gagcaagttt ctgtcgccgc caaaatccca gtgttcgaga aatcggtgcg ccagcgcgcc
     1441 gacgagcatt gccggcgcgc gttcttgagc gaaggcctga gttgtcacgc tcgcttcggt
     1501 gatctcttgt tcgcggcgct tgagcgaagt gggcgtgaca aaggtcgctg tctgtaaagc
     1561 tgcttgatac ttttcttcac ggtgccccca cgcctcgatg tagacctgcc aatcgcgttt
     1621 tttgtcgaga ggcttctttt tgctggtcgc ggctcccggc gcggtaaggc tcgcctcaac
     1681 cagttcaact gctaaccggc cgcagccgat ctcgatggtg gttgactgtt cggcgcagcc
     1741 gatttgatcc tgtgccgctt catccaacat gccgacaaag ttgccggtaa acttcctggc
     1801 ggcagggccg ctggagatga tcaaatgctc tcgagctcgg gtcatcgcta catagagtaa
     1861 gcgcttggtt tcttcttcgg ctctcaactt atttttttca gcgatgtaca caccggccaa
     1921 gtctgtgaac gagccgacac gaattccagt cagcccgctg gaccaatcga acagcgcttc
     1981 tgccggccgc ctctgctggc cattgatgcc ggcgtgacag ccggccagaa ccacaatggg
     2041 aaactcgagt cccttggatt tatggacact catgatgcgc acggcatcga catcttcttc
     2101 ggcgagcacg ctttcgcctt cttcttcgac atcgagcacg cgctgttgca gttggcggat
     2161 agccgctttg aatgtcatgg agctatcttc acgcccgagg agttcggcct gacgtcgcaa
     2221 cttatcgata ttcgccacag cctgttcgcc atgaaaatag caggcggcca aaagcctcag
     2281 tggcagcctg tcgaaaattt gcgatacggc agcgccaacc ggcaggatgc ggcactcggc
     2341 gtgcaatttg gctaaggcgc gataaagttc cgtcagcgcc ggtggaaagt ttttgccgct
     2401 aagctgctct gaacgccggt agtcgagcag attatcgcag tgcagatcgt aaatgttttg
     2461 atcgctcaag ccaccgatgg gcgagcgcag cacgccgacc agcgcgaggc gatcattggg
     2521 attttcgatg gcccggagca aattgaccgc atcgatgatt tctttagcgg catagaaatg
     2581 gcgctcgcct tcgacgacat agcgaatgcc ccggcgccgc agtggttcga ggtagtcatg
     2641 aatgtcggtg agcttgcgga agaggatcgc aacatctttc ggctgcgcca gaacccactc
     2701 gcctctggca ttgacgaagc cggcttcgtc caacacctga tcatggagcc agtcagccag
     2761 gctttcgccc tcgaggcgac gagcggtttc ggcgctgacc tctccatccg gcgccacgat
     2821 ctttcgcatc attgctttcg gcagaggctt agcgctgcca attccagccg gtgaatcgtg
     2881 cccgggcggc ggctgaattc cgatgtaagg cggctgcata ccgggtttcg gctgaatcag
     2941 cgatgaaaac accccgttga cgacatcgat aatcgccgta tcactgcgaa aattggtaag
     3001 caagtgacat tcagtgccgt tttgcgcttt gataatcttc tcgacgactt ccagataggc
     3061 ctcgatatcg gcgcgtctga atgcgtagat cgactgcttg ggatcaccca ccacaaaaac
     3121 ttttcccgcg gtaagtttaa ccttgcgcca atcggcggcc gtactgccgg gctgttcagt
     3181 caggtagagc aaaatctcat actgaatagg atcggtgtct tgaaattcgt cgatgagaaa
     3241 cgtccggtat tgccctttca attcttcgcg cacgcgcaga tggtcacgca ccaaatcgcg
     3301 cgcgcgaatc agcaggccgt caaacgaaat aaatccttca tgaacgaaag tctcgcggaa
     3361 acgcgcggcg tagggtaaga ggaggtgccg gatcagatga gtcaaacccg cgtcgactct
     3421 gcacaaggct tttgccgcgg tgactatgct gtgcgcttgt ttgatatcgg cctcgctcca
     3481 gcctttggtc tcccggttaa tggcgtgctc ccgtaaacag gctttatcag cgcctaaagc
     3541 cgttccgttt gcatccgcgc cgtcgataaa cgcctggata atcgagtggg cagcgcgcac
     3601 gagcttctcg ttattgcgat cttcgggatg gcgtttgctc aaactcgcgg cgctatggag
     3661 cagtgagcgc agccatgcgc ccagcgcagc atttgccggt tgattggctc ggaggggttc
     3721 gagctgaaca gattcggagc atagagatcg agcgagatct ttaatctgtt caagcgttaa
     3781 gtgtttgagc gccgcacgcc attcgccagc gcgagggctc tcgagcgcga gctcctgatc
     3841 gagccagaga tgccactgct ggtcgaaaag acggcggaac tgtttgccgt cgtcttcctt
     3901 aaactgcgga tccacgccgg cttccaatgg aaataggcgc agcaacgtcg ccgcaaagct
     3961 gtgaatcgtg ccgatctcag cgcgctccag attgcgtagc gcctcttgca cccgagcatc
     4021 gagctcgtcc ttggacaacc tataactact tatcaagctt gcgacttgca gttgtagttt
     4081 ttctcggtct tccccggggg gttcggctcg caggtccacc ttcaggtatg cttgaagccg
     4141 atcgcgtaac cgctgcttca tctcgttggc tgccttgttg gtaaaggtca aggcgacaat
     4201 gtcggtgatg agaagcgggt ccggattacg taacaacagg tggacgatcc ggtcgatcag
     4261 gagagttgtc ttgcccgtgc cggcccccgc agtgacgacc agattgcggt cgaaagtctc
     4321 gacggctgtg ctccggtcac gcgcatcggc gagaacggtc gccattgcgt ctgcgttcag
     4381 cattttttga tatctttctg gcgcaactgc cggtgcggag cggtgatcgg atcgttctcc
     4441 gcgcgccaga gactcggcgg gtgattcttg cggcagatct ccgcaatctc gcagtgtgga
     4501 caatactctc ccggttgcat gaagaattgg ccggcctgaa tgcccttgcc cagttgagcc
     4561 atgctgtttt tgatctcctc gccgatcctg ccggatagtt cttcgctatt gaatgatttg
     4621 ataaccaatg gaccgtcgct ccagcgcaat gcaatatagt aaaaactggc ttcgatttga
     4681 tattcgccgt tacgcaaact gtcgcggcgc cgctttgatt tacccagcaa agaataaaat
     4741 ggcggctgaa gtcgctgtcc gcgcaatgcg gcgcgggtca ggtcgttgtc ttccggcgaa
     4801 ggtttgccgc caaatttgaa tttgtaatcg acaacccgca atcgattgcc caaaggatcg
     4861 ctatcgatgc gatcgatgcg gccgtggatc gggagcgtgt tcaatggggc gggccaatcc
     4921 ggcggcaagc gatcatctat gccaacctcc aggtccactg gaacatagcc cgaagatgaa
     4981 agctcctgta gatcgcgacc cacaacctct ctgatgagtt gggtcagcgt ctcacgcatc
     5041 gtttcccaca tcagcggata cccgatcggg ttctcagcct catactgggt gaagactttt
     5101 tctgcagcgg cgctgataat cgaatcgaca gcaacaggag agcctctatc cgcgaaataa
     5161 ccgcgctgga tcagttcctg atacgtcagt ttcaatatta aatgacccag ctctcccatt
     5221 tccgccgcgt tcggtcccat ggcagtctcc ggcgcctcga aactctggag tccaagcagc
     5281 tggcgcgcga aatactgaaa cgggcagcgc ccgtaaagct ccaatgcagt aggcgagagc
     5341 ccgcgctttg agaagcgctg ccagtgcggt gccggtgtct ggatgatccc gtcgaagaca
     5401 tcgagccggt ccgtgctgag atctatggcg gcaatcgctt taagcccttg ctcgtacaag
     5461 ctgggcgaaa gactcgctgc tgcgattaaa gatcgcgaat ctgtcccgga aagattgaga
     5521 cgaatagcga gctcggccgg cagcagcgag tgttcaacat cgaatgggcg gctccttact
     5581 ttgtcgacgg tgctccgcgg gattgattgt tctacaaatt gttcctgctg cgcgcttgcg
     5641 agcgcggcct tgagctcggc gagataccat gaaggcgcca gcactctgcc gctttcatca
     5701 gagcgctggt acgaacaata gagccgctca gtggccgagt taaccaggag tgtaaacacc
     5761 agcttctctt cgtcgaaggc ggcgagcttt tggccgactt tatagccgag atcacgctcc
     5821 agcacctcgc gatcccgatc gcgcagaaac gcgtcttcgc gaatcggacg cgggaaaacg
     5881 ccttcgttca tgccgacgat aaacagcgcg cgaaatttaa taccgcgcgc ggcgctggcg
     5941 ctcgttacgg ttaccccggc tagattctta ttggtttcga caatggtgct gcgctcgagc
     6001 cagtgctgca atgtctcgct gaactcgcgc agcgagactt cggcctcgac tgcgtcaaag
     6061 ccggagattt gatccagaat ctcgatgatt tttttgcaga tcaaggtctc cgtgatgttg
     6121 aaacctccgg agggctccgg ctgaatccct aggtaggcac cgaacatttg tttccatgag
     6181 tcagcgtaat ctgtccagga agcgcgcgcc ggcaagcggc ttaaaacgtt atcaaggcct
     6241 ttaagaatag ttgccagcgc ccgcaactgg gttgcaggga tctcgataaa gcgccgctcc
     6301 tcgtcatcgg atatttgcga tagcttcatg ccaacccgga catagcggtc gatcctttgc
     6361 cattccttca tccctttgca gatcgccagc tctcgagtgg ccaggtccca aagatcggga
     6421 cgaagctcga tttccggctg tgccgcgccg cttaagcgga aataaggcga cgacaccagg
     6481 tcgatcacgt gggagcgcag gtagtctctg gcggcaaggt tcatcaggag tatcgcagct
     6541 ttggtcaaag gaaattgcgc tatcgattcg tctatggaac cggtaattgg aattcgatgt
     6601 tgggcaaaca cctcttggat cttggcgccg tagccctcca gagttcgcgc caccacaccg
     6661 atctgatcga atgccatgcc gtcctgctcg gtcaggagca ggatctcctt ggccactgcg
     6721 ctgatctcgt catcaattcc aaaggtattg gtgatccggc aatgccagtt ggccggtaac
     6781 ggttggtatt gtcgatgtgg atcttgatca aaaacagcag ctatctgagg caagtcgatt
     6841 ctgtgatcgc actcggccgg aggattgttg gtcgatgggt tatccgctcg cccctggata
     6901 tacactgcgt aaaagcgctt ggcgaattgc caaccttcat gcgccggctg agtctctagc
     6961 agcgggaaaa acagagttgt cggcacggct cgtgcgacgg catgaacgag atccacctgg
     7021 atctgggtga gatcgtagaa gccatagtag aaaatccgcg cgaactggcc taaaaagtcc
     7081 gacgccggcg ccgtcgcgat tgccgatttg tccaaggtag aatgatccaa aatcccgtgc
     7141 tcctggcaga agcgaagaaa cgtttgaagc atcaccaaga gattggctgt cgtttcgtgc
     7201 gttcgccgat cgaactggtt ttcacggaac gcttccaaag cgatatccgg ctgtaccagc
     7261 ccatcgcgta gatctctcag gctctgccag agcgcggcgc atccgccggc acgttcctcg
     7321 atgccgctga aagctgccgt gccgggttgt tgcgcgcgga taatttgacg caatgtctct
     7381 tcaagaaaaa ggtcgtcgcg caagaccggg ggattgagtc ccttggcttc tgcgaacagc
     7441 tttgccgaca gttggtgaaa cgtcagcagt tgtagattta tgaaggataa gtttcgttcc
     7501 ctgattagca atattttaag atgtcgccgt agcgcatcgg atgggactag aatcagcagc
     7561 ggacaaagtg gatcggtatt cttgtagtgc agaacttctt cgacaaggga attttccagg
     7621 tgcggatgga agggagcgag aagggcttgc atcatctttg gaattgttgg ccagacgcac
     7681 ggctaatatg gaataaggga tgagctatgg caagcataaa ccgcgcgggt actcactcgg
     7741 taattcgcga aagcgcgcga cgcaacttac cggtgttatc gagtgcaggt aaataagttg
     7801 caaatcccgg aacaaagaac taagaatgat gagtttatgc gctgcctgat tcccctgcgt
     7861 ttcgttttca tttttatttt cctatctaac actttttttg ctccgtcgtt agtgcttgcg
     7921 caacgcgcca ctggcttggt ggtgtcggat agcccattgg ctacccaggc gggaatggag
     7981 atgctcggcc ggggcggcaa tgcaattgat gccgccattg ccactgccta tgcgttggcg
     8041 gtggtcgacc aagcttcatc gggaatcggc ggcggcggct ttatggtcat ttacgaaacc
     8101 aaggatcgtc gcgcgcatgc gctggatttt cgcgaagccg ccccggaggg gacgcgcaag
     8161 gacctgtata tcaaggacgg caagcctgtg ccctctttga gtcttaccgg cgcgctggcc
     8221 gtggcggtgc ctggcgaggt cgccggtttg gcgcaggccc acaaacgctt cggcagcatg
     8281 ccgatgcagg tgctgatggc ccccgctatc agactcgcgt ccaacggatt tccgctggac
     8341 gccactttgc gctacgccat cgatcgccag cagggttcga tgaaaaagtt tgccgatctc
     8401 gggcgcatct acatgccaaa taacgaggtg ccggcagaag gcgaattgat tcgccagccg
     8461 gaactcggtg acaccttaaa ggcgattgcg tctcaagggg ccgacgcttt ctatcaaggt
     8521 tggattgccg acgcgattgt cgaaactatc aagagagaag gcggcgtttt aaacagcgag
     8581 gatctgaaaa attaccgtcc ggtatggcga gaaccgctaa tcggcaatta tcgccagcga
     8641 acagtcattg gcatgccgcc gcccagttcc ggaggcgtgg cgattttgca aatgctcaaa
     8701 gtgctcgaag gctaccatct aagcgctttg ccgcataatt cggcaacgta tctccacctg
     8761 attactgaaa ccatgaaaca cgcctttgcc gatcgcgcgc aatatcttgg cgatccggat
     8821 tttgtcaagc cgccggttcc gctacttacc tccaaagatt acgccaggtg gattcgcggc
     8881 cgcatctccg cggcgaaaac tcagcccacg cgatactatg gcctggttaa tttcaaaccg
     8941 gagctgggtg gaaccacaca tttcagcgtg atggaccgtt ttggcaatgc cgtggcatgt
     9001 accatttcgg tcaacacgcg ctttggctcc aaactcttgg tgcccgatac cggtatcgtg
     9061 ctcaataacg aaatggacga tttcgcgata cattcgaccg gcaatgttta cggtctggtc
     9121 ggcaatgagg cgaattccgt gctacctagg aagcggccat taagcagcat gtctccgacg
     9181 atcatattgc aaggtgatcg gccggaactt gtggtgggcg gggcgggtgg ccctcgcatc
     9241 atcagcgcca cgttgcagac gattctgaat gtgttggact accgaatgcc ggtggccaag
     9301 gccgtggaag ccgctcgcgt gcatcatcag tggttgcccg atcagttaag cgtcgaaaac
     9361 gatattccca tcgaaaccag gagatcactc gagcggcgcg gacacgcagt acgcgagcgc
     9421 ggcgttctcg gcgtggtgca ggcgatcacc gccgggcagg atggggtcaa aggagcggcc
     9481 gatcctcgaa aagaggaaag agcgcgatcg gagtgagtgc gttagtcacg gaaccaaatt
     9541 cgcggaaccg cgtaggtcac cgagctcagg tttaagcgct atcactctta tcgagaggtg
     9601 gcgcagcttc caatctggcg atttcgatcg ggtctaactg aatgtccgtg cgctttccag
     9661 aaggtctcga atcactcaac tacgacggat agtcagccca accagaagac ctatgccggc
     9721 ggcaatggcc actgcattcc acggattttc ccgaatataa tcgtcggcga tgtcggcgta
     9781 ttctttggaa gtctcaaata gcgcttgttc ggcatcggcg agcgccttct taccttcaat
     9841 caagctttgc tcgatttttt gtcgcgccgc ggccactttc tcgcccgcct ggctggtggt
     9901 ggctcttaac agctcttccg cgtcgctgac catagtcttg agatcattga tgagcctttc
     9961 tcttgaggtt tcgccgttca tctgtcctcc tccggttctg cttttaacgt agcccaagat
    10021 aaacacgagg ccagcaggta ttcaagcttg gatgctcata cgaccaggtt gtttcgcggt
    10081 tttaatatgt catcgaaaga tccggagaac taccaggatt tctctgcacc gatcaaacag
    10141 tactggacat cttcaaacta cgatagccat gatcgcgcgg tagcggctgg cttgggcccg
    10201 acttggcgct agctgtcgaa aggatttttc agcaggatgg tttgttcccg gccgggtccg
    10261 acggaaacca gaataatttc agtatccaga agcttctcga tgcgctgaac atagttctgc
    10321 gcggctgatg gcagctcgga aaatttcctc actccggtta tcggttcgtt ccatcctgcc
    10381 atttcctcga gaaccggttc ggccttgtcc agcactttcg aactggctgg aaattcatcg
    10441 tatagccggc catcgaaccg gtatgcggta cagatcggga ctttcttgaa accggtcagg
    10501 acatcgagct tggttagagc gatgccggta atgccattga cgcggacggc atgacggacg
    10561 ccgacggcat cgaaccaacc acagcgccgg ggacgtccgg tcgttgcgcc gaactcgacg
    10621 ccttcgcgtt ttaagatttc accttcgggc ccaaactgct cggtgataaa tgggccgctg
    10681 ccaacccgtg tcgtataggc ttttgagatc ccgatcacat gatgaatgtg ttgcggcccg
    10741 actccggcgc cggtgcatgc gccgccggta gatgtattcg aggaagtcac ataagggtag
    10801 gtgccatggt cgacatcgag caaggttccc tgggcgcctt cgaacatcac ttttttgccg
    10861 gcgcggatct cgcggtgaag caataaactc gtgttggcga tgagcggccg cagccgttca
    10921 cggtagccgg cgtaagtgtc atgtatgtga ttgaagtcga gagtcttctc tttgagaatt
    10981 gctttgagat agatgttctt ctcttcgata ttatggcgta gtttctctcg aaaggtgtcc
    11041 tcttcgagga gatcgataaa ccggataccg acccgcgcta ctttgtcctc gtatgccggc
    11101 ccgatgccac ggccggtggt gccgattttt cccttgcctc tgagccgttc gcgtgccaga
    11161 tcgatggctt tgtggtacgg cataatcagg tgaccttctt cgctgatacg cagcagcgaa
    11221 tcatcgagca aatgcccttg ggccttgagg gcgtcgattt cgtcgatcaa aatttccgga
    11281 tcgaccacca cgccgttgcc gatgacgcat aacttatcgg gatgcagcgc cccggagggg
    11341 accaaatgca gaacggtctt cttaccgtca accaccaagg tgtggccggc gttgttgccg
    11401 ccttggaacc ggacgatgat atcggcgtcg caggtaaaaa ggtcgacaac cttgccttta
    11461 ccttcgtcgc cccactgagc tccaatgatt gctacgttgg acatttgatt aggcgatggg
    11521 tttatagttt gactaattcg gctgaaaccg tttgcggcag attgcgcagc tctttcatgg
    11581 tctctttggg aatctgatca tcgacgtgaa tgaatgaaat ggcattgccc cctttcgcgc
    11641 cgcgtccgag ctccatgccg gcgatgttga tgccgttttt acccagtatg gtgccgacat
    11701 ttccgaccac gccgggcacg tccttgttgc gcaaaataag gatatagcct tccggcgccg
    11761 cttccagata aaaattatcg acacgaacaa tgcgcgggtg cttgtcgccg aaaatggctc
    11821 cctcgatttc gacttcacgg tcaccggtag cgatcttgac ggtgatggaa ctggaaaagt
    11881 cgccggcctt gctgctcttg gattcaacca cctgaatgcc gcgttctttg gccagtatcg
    11941 gcgcgttgac gtaattgacg ctcgactcca tcaccggagt aagcatgcct ttgagcaccg
    12001 ccaacgtgag cggagcaatg ttgtattggg ttacttcgcc gcgatattcg acgtgaaccg
    12061 cctggggcag cacatcggcc atttgcacct ggagactgcc gagcttttcg gcaaggatca
    12121 gatacggccg gagaatctta aaaagttccg ggctgacaga agggacgtta acggcgtagc
    12181 gaaccagtcc ttcggtaaga aaatccacca tctgttcggc caccgcgatc gccacgttca
    12241 gctgggcttc gtcggtggat gcgccgagat gcggcgtggt gatgacctgg tccatattga
    12301 gcagagggtg gtcggcgggc ggcggttcgt caacatacac atccaaagcg gctccggcca
    12361 cttttccttc tttgatggct tccgctagat ccttttcatc gacgatgccg ccgcgcgcgc
    12421 agttaatgat gcgcacgccg ggtttcatct tggcgaaagc ttcgcgattg atcagcccct
    12481 gggtttcatt cgtcagcggg acgtgaacgg tgatgaaatc ggacttggag aaaatttcct
    12541 ccagcggcgc cggctccacc tccatgcgcg cgatattctc ggctgagatg aacggatcgt
    12601 agccgaggac tttcattcgc aggccggcag cccgttcggc aacgatccgg ccgacattgc
    12661 cgaggccgat cacgccgatg gttttgttgc ataattcgac gccgataaac ttgttgcgtt
    12721 cccatttgcc gttcttcaga gaagccaccg cctgcgggat gtggcgcgcc agagccatca
    12781 tcaaggatat cgtatgttcc ccggtggtga cgttgttgcc tcccggggtg ttcatgacga
    12841 cgatgcctcg ttgggttgcc gcatcgacat cgatattttc aaaaccgacg ccagcgcgtc
    12901 cgatcacttt caggttcttc gccgcctcga tcagttcagc gctgatcttc gttccactgc
    12961 ggatcaccca accatcgtaa tcagcgcaaa tcttcttaag ctcgttagga tcgaggccgg
    13021 tccgcacatc cacttcaaag cccggggcgc gcttaaatac ttccaagcat tgaggcgcga
    13081 gagaatcgct caccaggatc ttcatcgtta aaccaaagac tccatgagaa tttcttgtgc
    13141 cgccgctacg cctctgccga acggaagctc gacgccgaat ttgcgcatga ccatttccag
    13201 cgccgcaacc gcggtgatca cgtcgaaggc tcccatataa ccgacgtgcg cgattcgaat
    13261 gacttttccc tttaactgat cctgtccttc ggcgagcgta atattcatgt gatcgcgcag
    13321 atagtcgagc acctggtcgg catcgatacc atccggcagg tagacgccgg tggctgccgg
    13381 gctgggattg tcgggagcaa gcagcttcaa ccctaacgcg gtggcagccg ctcgggtggc
    13441 tcgtgacatg cgcgcatggc gggcatagac ccgatcgaga ccttcgcgtt gcatcatctc
    13501 gagcgaggcg cgcgcgccga aaatcaacga caccgccggt gtaaatgcgc cggagccttt
    13561 ttgttggttc ttgcgctcga gattcaggtc aaaataaaag cgtggcaatt ttgcttgttg
    13621 ggtactttcc caggcgcgat cgctcagagc gatgaaacca aggccgggcg gcaacatcat
    13681 cgctttctgc gatccggtca ccaaaacatc gagtccccat tcatcgaatg gtatcgaaac
    13741 gacacccacc gccgtgaccc catcgaccac taatagcgga ccgttcttgg ttagcttggc
    13801 gatctctcgg atcggatgaa gcacggtggt ggaggtctcg ctggcctgga ccatcacgcc
    13861 tttgagatcg ggatgggcct tcagttgctt gtcgaccgcg tcaactttta ccgcctggcc
    13921 ccattcaact ttcagctcga tcggattcag cccaaaagca ttagcgatat taagccagcg
    13981 ttcgccgaat tttccgccat tgatcaccag gaccttatcg gctggggaaa ataaattggc
    14041 aaccgatgcc tccatagctc cggtgccgga agaggcaagc atcaacacgt cacttttggt
    14101 gccgaaaagt tttttgagtc cctggcgcgc ctgatcgaaa accttgttga attgcggcgt
    14161 ccgatgatga atcatcgtct ccgacatggc caaagcgact tcattaggaa tcggggtggg
    14221 accgggcgac aataagtagt gcttgatcat cggtttttac ctctcagagc tctagcaacc
    14281 ctgcgagttt tcggcagaag gaggagcaag aaacgtgcgc tctgagccga tttgagattt
    14341 cttagccggc agcgagagcg atgcgcggtg aagttagttt gttcgcaggg cataaaaaag
    14401 cctgaacctc tatctgtaag agacccaggc gttcggccgt cttagtgctt tgcactcccc
    14461 cgtggttaat tccacgttga cgccagtcgc gcaaaactta cataatttat ctatcgtaca
    14521 ccctatacct tgtcaacggt ttgcgatcgc tgccactcgc gccaatttgc cattaccggt
    14581 tcgatttgcg actatccgat gctcgatgaa acgattgtta ctcttcgata tcgacggcac
    14641 cctgactcga agccacaacg gtcacatacc ttttaaccag gcgattctcg agacttttgg
    14701 cgttccgggc gatatccgtt cggtcattcc cgatggcaat accgacccga tgattgtcac
    14761 cgaaatcttc agcaaaagta atattctgat cgacgacatc gatgataagt ggcaacagtt
    14821 ctcaagcaac ctgcgtcaat gctattcaca ggcgattagc tcgggaaata ccagcgtgcg
    14881 cgccttaccc ggaactgtcg aattgctgca aacggtcggc gtaggcggca ttttcggcag
    14941 cagtgtcgtc agcggcaatc tcgaaccaat ggcacgggtg aagctcgaaa ccgccggtct
    15001 ttattcttat ctctgccgcg gcgcttacgg cagtgattcg ccgcgtcgcg ctgatctgcc
    15061 ggcaatcgcc aaacaccgct gcgaagcggc attcggcgtc tcgataccgt tcaacgagtg
    15121 cattgtcatc ggcgataccc ccagggattt cgaggccgcc cggtgcaacc gcatgaaatg
    15181 catccttgtc gcaaccggtc gttatccgct agcagaatta tcaaagtgcc aagccgaacg
    15241 gtgtttggcg gatttgaccg acacggccgc agttttagag acgcttttga acatttagca
    15301 ggttgctcaa aaagacctca gaagcaagcc gatctcgtgc ttgtgttcgt ggaaaacgtg
    15361 atcgagaatt ccgcaacgag cacgaatacg ggccacgaac acgatttacg atccgatcga
    15421 gcggcctgga atcagagttc gagaatctga ctatccttag ggaggtgcca ttatgcaatt
    15481 tcgaacgatc ggtaaaaccg gagttcgagt ttcggaaatt ggcttcggct gcggcaataa
    15541 tgcggttttg atggtcaagg cgagttatga agatcaggtc aaggcggtgc gccacgcgct
    15601 tgatctgggc ataaattatt ttgacacggc gtttgcctat ggattgggga aatcagagga
    15661 gaatctcggg aaaattctca atcagctgaa aaccccggca gtgatctcga cgaaaatccg
    15721 gctcgaggcc gacgcgttga ctgatatcaa agcggcaacg cagcgcgagg tcgaggccgg
    15781 gctgagacgt ttacaaagag attctgttga tctgattcag ctgcacacga gggttgtcac
    15841 cggtcggcag caagacaaac gctttggcat gacacccggc gagatactgg gccgttcggg
    15901 agtaatcgaa ggtttcaaag cgatgcgtga caaaggaaag gtcggctatt ttggtttcac
    15961 cggtttggga gatcccgcag cattgcacga agttgtcgat agcggcgcct tccactcgtt
    16021 tcaagcgtac tacaatctgc tcaatcctag cgccgggcat cccgttccgc ccgcattcag
    16081 cgctttggac tacggtcagc ttattgatcg cgccgccgcc cgaggcatgg gggttgcggt
    16141 gataagggtt ttagcggccg gcgcgctcac ttccaatccc ggcgcgggcg gtggcagtac
    16201 cccggagcct ttgtccaaag gctcggaata ctcgctcgat gtacaacggg ccgaaaagtt
    16261 gaaagccgct cttgccgcag atggcaaagg aatgacccaa gccgctattc gcttcggatt
    16321 gatgaatcca aaagtttcga ccgtgctggt aggcttttcc aacagcgccc atatcgatga
    16381 agcggtggca tgctccggcg cgccgccatt atcgaacgaa gagatggcga tgatacaaag
    16441 gctttggcag accgatttcg gcagatagaa ttaaagaggt taacgtggct ctgttctttc
    16501 actccgacga agtcgatgaa ttcattccgt ttacagaagc tgttgccatt tccgagaatg
    16561 ctcttcgcaa tataaaaact cccgccggag ttaatgctcc gcgcaaacgg ctcaacctgc
    16621 atcgcgagat cgccgaaggg agcttcgaca ccgttctcaa catatacgcc ggcggctctg
    16681 cggcctacgg cgcaattggc gcgcaggtgg cgctccatcg caaagcgatt gccggaaaca
    16741 tgcaacgacg gcctccttac aatcccgatc aaaccgaact cgcgctaatc tacgatactg
    16801 acacgggatc cctactcggg atcatggcca accggccgcg ccatgtaggg ggcgccgccg
    16861 atctgcgcac gccagcggtg agtttagccg gcctggagca gtttgccagg cgtgatgcca
    16921 ggcgcgtcgg catctacggc tccggccaac aagcttattc gactttcgtg ggattaaccg
    16981 agatgaggaa aatcgagagc gccaaaattt atagtccgac gcccgcgcac agggaaggtt
    17041 ttgccagaaa aatcagtcag agaaccggtg tgccggttcg agccgtggaa aatccagagc
    17101 tcgccgcgcg ggacgtcgat attatcttgt gcatgactaa caccaatgtc ccggtgatcg
    17161 atggatcgtg gctcgagcag gggcaatata ttatctccgt ggtcggcagc aacatcgaac
    17221 tggtcaaaag cggcaacatt gctgcgccgc ggcgcgagat tgacgacgag actctcaacc
    17281 gttgtagttt tatcgtcgcg ctatcaaagg aacaggctat cgattcgcag caaggggata
    17341 tctactggcc ggtgcagaac ggaatcatgg cctgggataa ggtaattgac atctccgata
    17401 ttcttaccgg gaaagcgaaa ggccgcactg atgacgagca aatcatcctc tacaagaacc
    17461 agggtgggca gggcatcgtc gacatcgctc tggcgaaaaa gtgtttcgat cttgccaaac
    17521 aacacggcaa aggtcacgag cttcgcatcg aagcgcgtga gaactggtgg gtgcagggag
    17581 gaagaactca aacgtggtga ggttcgacag cagattcgtt ttacggaaaa aaaggaggtc
    17641 ttgatgattg gcaagtctag tggctgcacg gttcaagggt ttaaacgttc aaaagctcaa
    17701 gggagcaaat caagcacaat tgcgaacacc ttgaaattca tcttacgtct ggcggctttg
    17761 atcatttttc atttgactat tcctttcacc gtccgtgccg tattcgccca aactccctac
    17821 catcagggca aaaccattac catcgttgtc ggcaccgtgg cgggtgatct ctatgatctt
    17881 tacgcgcggg ccatcggcct cgtcatggga aaatatattc cgggaaatcc caacatcatt
    17941 gtgcaaaaca tgcccggcgc cggccatatg attgcggcca attacattta caacgtcgct
    18001 aaacccgacg gtttaacgat ggcggcgatc aacgcgggtc tctattttga gcagctgatc
    18061 gggcggcccg aagccaagtt cgattggacc caatacacct ggatcggcaa cgcgacaaaa
    18121 tctccccagg tgctctacat gcgcgcggat agcccgtaca aatcgatcga tgatgtgcgt
    18181 aacgccaaag agccgccgaa atgcggcacc acgggtatga gcaacatggg atacttcgtg
    18241 ccgaagctgt tagaggaaac cgttggtgca aagttcaacg tcgtcgctgg ctatcaaggc
    18301 ggaaatgagg tcgatctggg cgtggagaga ggtgagatcc agtgccgctc gcttagctct
    18361 gaagcgtact tttcacgaga gccgtttctc agttggcgca agcgaggctt tgctcgcgaa
    18421 ctggttcaag gtgggcgttc aagggatgag aggatgccgg cggcgccgac tatttacgaa
    18481 ctgatggaca agtacaatac ccccgaaccg gcacggcggc tggcaacggc tctgctcgcg
    18541 tctggagatt ttcaccgccc ctatttggca ccgcccaagc tgcgccccga gcatgtgaaa
    18601 atattgcgtg aggcgttcaa caaaaccatg aaagatcccg aattcttggc cgaagccaag
    18661 aaaaaacggc tcgatatcga tccgaccagc ggcgaggaag tggaggcgtt aaccaatgaa
    18721 gtgatgtccc agccgcccga ggtaatcgag cgattgaaga agctgatggg acgttagaag
    18781 tggcccactc gggcagtaag actcttgcag tttcaaagtt atggaacccg gcaccaacgg
    18841 ctaactagcg ctcgggcttg cgcgttccaa cttgctgccg ctctgagtgt tactaaagtt
    18901 ttgcctgcgc cttggctttc ttatcctccg gcgctttgat cttatcgaga ttctgcaccg
    18961 cataatcgcc ggtggtgacc acttcgtcct tgtcgctgaa acgcggttta gcggcggcct
    19021 cggcgcgcgc cccgagcagg atcatgtact cgtcgcccgt gttatcgagt tgataaagtt
    19081 gaccggccgg gatggtcacg agcatgccgg gtgtgagttt tcttgccggg gtatcgggaa
    19141 agtgaaacgt gcattcgccc ttgacgcaat agaattgctg atcggccgtg tgtttgtgca
    19201 tcggcccctt ggttcccgga tagtcgccat gcacccaggt atgaaagtga tccgaagtga
    19261 acagaacttt tttactatgg ccaatgtcca gttccggcag atttaacaaa tcgatgaatt
    19321 ccgcggccat ggtcggctct cctatgggtt tgttgagact gatcttgaag tgagttggtg
    19381 cctcactcct ggttacaatt gtaagttttt gcaacaatcc tgcattggat acttcctcat
    19441 agcgatttgt cgattacccc aagacatcga gcgtccttcg ttccacggca gctgcggcga
    19501 cggcgttttt gatttcctcg agtttgtaag gcttgaagat caccgcgctt agatcgccgt
    19561 tgtccaatgc agattcgact tcagcggaaa cataaccgga agctaggatt ggttttatcg
    19621 tcggatcggc tattttcatt tgcttcagaa cgtcccagcc attcaattta ggcagtccta
    19681 gatccaagac cacgaccgag atttcgtcct tgtactgcct gaaagtctcg acagcctcga
    19741 caccatctag tgccgtcaac actcggtaac cggcggactc aagataggcg atcatgagcc
    19801 tgacctggtt cttttcatcg tcgacaaata gcaccaactg gcctttcgtc gaaatacttc
    19861 ctggcgcttc agaacggctc gatattcgcg actcagggtt caactgggag gtctcggcaa
    19921 gcggcagata gatacgaaaa gcggcacctt gacccggctc gctggtgact tcgatgaaac
    19981 ctcggtgatt ggcgataata ccataggcga cagccagtcc cagtccactg ccttgacctt
    20041 ggggtttggt ggtaaaaaaa ggttcgaata tgcggtgctt cacggtttcg tcgattcccg
    20101 atccggtatc ttcaagagta acgcacgcgt aggattgctc tttcgcgtcg gcgtaggtct
    20161 tgcgcagttc cacgccggca atcacgtcgg ttgcgagacg aagctttccg ccatgcggca
    20221 tcgcgtcgcg cgcattgaga caaacattta aaaacacctg gtgcagctgg ttgggatcgg
    20281 ccatagattg cggcaggctg ttcttcaaat ctacctctat gtcgatagtc ttgggcagag
    20341 tgccgctcag aagacttttc aaattttcca acatctcatt aagatcgatg ggctcgaatt
    20401 gaaggttgct ctcccgggct agcgcgagca actgccgcac cgtcgctgcc ccgcgctcga
    20461 ccgtctcgtc gatgacttgc aaggcctcgg cggccatttg ggggtcctca gtggtttgca
    20521 gcagcaggga agcataccct ttgatgatgt tcaaaatgtt attgaagtcg tgcgcgatgc
    20581 cgctcgcgag agttcccata ctctccatct tctgggcttg ccgcaactgt tgttcgagcc
    20641 tcttttcttc ctctcgctcg gcgaaaagcg cggcgttggc catctgtagt tctgcggtgc
    20701 gcagttgaat tcttttttcg agcccctcgt tggctcgtgc cagatcatct ctggcgcgct
    20761 gcagggctgc gtcaccctgt ttgcgatcgg tgatgttcaa aaagatgccg atgcagcccc
    20821 gtaccttgcc gtgctcgtcg cgcagaggag tcgcgcgaca gagctcgtgg atgatgctgc
    20881 cgtcttctcg ctctatctca agctcttcat ccactacatc gacgccttcg cgcacagctc
    20941 gctgcatcgg taactcttcc gcgggaagct ccttgccgtt gcggaaaatc ttgaaagaca
    21001 attggtcttg ttctgcgccg gtcttcgaca ggttctggtc ggcaccggtg ccgaccattc
    21061 gcataaattc cggattgccc caaatatgtt tggattccga atcggtagcc actgcaatac
    21121 cgatgggggc cgtatcgatt agtttttgaa attcctcgac acgccgctga acggtctgat
    21181 acaaattagc attgtcaaat gcgaccgatg cttgtgcggc gatggcggtg acgatgcttt
    21241 cagcctctgc ggtaaagaga ttaggttgtg aatggccgaa tagtaatcca ccgataacgt
    21301 ctcccgagcg cgagcttacc ggaaccgaca ggtaactccg cgccggcagg gcgtcttcgg
    21361 cgctcccttg atccggcaac gtcgttgcaa agcgcgggtc attcaagaca tcgccgatgc
    21421 gcagcacgcc tccggcaaac atcgatccaa agatctgtcg gttgcgcggc atcaatttat
    21481 cgaccacgtt gtgcggcgcg ccggataaag aatagagcgg aaacgaattg ccggatttat
    21541 ttgctacatt gtagaagaaa ccaccgaact gggcaccgga gatttcacgg ccggcatcga
    21601 tgacgatttg cgcaattttt tttaaatcca gctcggcagc caatgcgtag ccaaccctgt
    21661 tgataatttc cagtgagcga gtccgctgcc gcagcatctc ttcctgatgt ctgctttcgg
    21721 ttatatccat gcagacgccg gctaacttct ccgggtgccc gtcggccccg taggatagac
    21781 tggcaagagc ctctacccag cgaacctgac cgtcagggtg gacgatgcga taggcgatct
    21841 cgtagtcgct tcgggtttcc aatgttttct gaagttcctt cagaactgat tcgacatctt
    21901 cggggtggat agtttttttt aaatcttcga aggcgcctcc gaagctgccc cgcttcaagc
    21961 cgtgaattac ctctaaactc gccgaccaag tgaccttgct cgcgcttatg ttccattccc
    22021 acgcgcccat ctgtccggca gacaacgcca gttgaagtcg accttcactt tcgcgcagcg
    22081 cggcttccgc acgttgccgt tcgatgaact ggccgaattg ggtgcccaga ttagcgacca
    22141 tctcctggaa ctcgtcgtcc ggttctctcg tttcgcggct aaaaaactcc aacacgccga
    22201 aaacctcgtt atcgagtttg atgggaaaac cgacagctcc atgaaagcct acgctgattg
    22261 ccacgggcgc gcgcggaaaa ttattatctt tgagtacatc gctaatccaa gccggggttg
    22321 cgctctccca gacgcggcct ggcagacctg taccccgctc gaacgtgatc tcacgcgtga
    22381 ccgctgcaaa tcctgataga tcaatggaag ggaggcacca catgtcgaca caggtgagta
    22441 cgttcgaatt tcggttgacg agccataatg cgccgaaatc ccaggcggcg gtttcacaga
    22501 tagctttgat gacccggggc gccgcctcgg ccatagtcga agcctcggaa aagatgcggc
    22561 tcacagcgtc tttaatcctg agacgttgct ccagacgctg ctgttgcgag accactgaag
    22621 cgacgatgag agctgtcatg ctgattgtcg cgataaacag ctgtagtaaa aatatcgatt
    22681 cgtgcggttc cgatggcgcg aaaggcccga gatcccgcac cgtgccccag agtgcaatcg
    22741 cggatgtcac gaatgccgcc gtgacggcgc ctcttcggcc gaagcgcaag gcggcccaga
    22801 gtagcggccc gagagcaaaa tattccaagc cggcgggaac cggattaaga aacagcagcg
    22861 ctgacatcga aatgactgcc atcaacaagc ccgcagcctc gaagatatgt ctggatcgga
    22921 caggcagtac aggctgcgtt agccacatca gcagcagagg agcgataatc aggtttccca
    22981 ctgaatcgcc gagccaccac gttagccaaa cggtggagag ctcgggcggc gcgatgaagc
    23041 cgccgagata caaagtagtc atacccagcg tcgcgccggc agcagtgctg atgacgccgg
    23101 cgagagctat aaaaatgaga atgtttctag tgcggtcaaa aacattgacg ccctgggcaa
    23161 agcgagctaa caacgtcgct gccag
//
LOCUS       DASUVS010000191        10793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_77451, whole genome shotgun sequence.
ACCESSION   DASUVS010000191 DASUVS010000000
VERSION     DASUVS010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10793
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77451"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            553..1353
                     /locus_tag="VF182_10640"
     CDS             553..1353
                     /locus_tag="VF182_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX6437579.1"
                     /translation="MMPKYCVGFIVFIVGAVVGSFLNVCISRIPNGQSVIQPPSHCPK
                     CQAPIPFFYNIPLISYLLLRGRCCSCGDRISFRYFAVELLMASLAVLLFYQFGLGVAF
                     VVEFVFVAALIVISFIDLDVRIVPDVISLPGIVIGLLFSVIGHYLIRDPLHVVPTPLS
                     AFIGVLVGGGFLLAVAWIYEKFTGVEGMGGGDIKLLAMIGAFLGWPSIPLTLFFSSLS
                     GSVVGLTIMLAKGVDRRYALPFAPFLCLGALVYLFFGREIIDYYVPPR"
     gene            1489..1935
                     /locus_tag="VF182_10645"
     CDS             1489..1935
                     /locus_tag="VF182_10645"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEX6437580.1"
                     /translation="MDCQSRVGFSLIELIVVLSLMGILFAIGIPASNKLLLSYQLNGS
                     ARRVQTDLQNIKMRAAAENLGFQFTYGAGSGSYTIRRDGAVFVSKPLPEGIVITQAGT
                     VSFSPRGTAGSNRIRLQNRGGACKQVVVSSTGRIRVCNPAGCGEDC"
     gene            1938..2348
                     /locus_tag="VF182_10650"
     CDS             1938..2348
                     /locus_tag="VF182_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437581.1"
                     /translation="MLLRFPARKRYEDGFTFNEVLLAMNVIVIGILGYSVGTGNLIRW
                     NSASRDYTVAVNLAQDKLEQIKAQKNWPDVNNCPASGDHGIAATGEPGGQFNRCWVIA
                     DSTLGAELKEITVTVSWPGRESREVTLTTLLYSG"
     gene            2495..3004
                     /locus_tag="VF182_10655"
     CDS             2495..3004
                     /locus_tag="VF182_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437582.1"
                     /translation="MATLEDARGAMDIISRDLKLAGSWGTGSAPSETGLGDDPDADDD
                     SACNRIYRASERVIQIQMDLNGNGNCADLEPRENIRYELGAATTNCPGRTVIRRNGDC
                     LVAYVTAPNSGKLFKYYDENGLLLSDTPPLAAIKRIVISFSVGMKETDFKTGASLGSA
                     LSSSVELRN"
     gene            3004..4191
                     /locus_tag="VF182_10660"
     CDS             3004..4191
                     /locus_tag="VF182_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437583.1"
                     /translation="MKASDAYSKALVADQRGIVLYSSLMLLALIMAVGVQAIVSTQSN
                     FQISANLRAENVAFYISEAGIEWSKNELAKATAHPVPPFNSSSTISSGAFSVATISTI
                     AVSPLVSRMVVRSMGRFASSSQIVQAELIKRFLLADAAVGLRGNASHPIFVGNDFSIS
                     GIDHDPATSKPMASLRSYAGFSVSHPATKEAIEASLEPNQLSRVSGTDRQGALIAETQ
                     KLPSPAIAQLTGDLCSAENAQLTAVPAGTSLSLTDQQWGDRSVPQLRCLDGAPGSADA
                     VVKLSNSSGAGILIARNADLILEGGFRWEGLIIITGDMVSLKILETGNKEIIGGVIIN
                     ETGTFSETNPSTLEVHGDIKILFSRSALHTAGTVVPASALTTGHASLPVEVVQSYWRS
                     ITP"
     gene            4345..4728
                     /locus_tag="VF182_10665"
     CDS             4345..4728
                     /locus_tag="VF182_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6437584.1"
                     /translation="MDTQRTIMVVDDNPDIITIVKTILEGKGYHVLSASSGQELLNLL
                     TDGKPDLIILDIMMPEMDGLEVLGRLKAVTETASIPVILLTAKVQYEDVLGGYKLGAD
                     YYITKPFTSTQLVNGINLLLGESKN"
     gene            4780..5475
                     /gene="tmk"
                     /locus_tag="VF182_10670"
     CDS             4780..5475
                     /gene="tmk"
                     /locus_tag="VF182_10670"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX6437585.1"
                     /translation="MVQRYTLGETVLLINPMVGFITFEGGDGSGKSTQIRLLEKHLVM
                     RDKTCLTTREPGGTSLGRLLRQILLETGKRPISASTELFLYLADRAQHVHEVIAPAIL
                     KGTIVLSDRYIDSTVAYQGYGRGFDLQLLLSLNRIASEGLTPDITFLLDCPVQLGLSR
                     TVHRGSNPSTSKSNDRFESENLEFHEKVRNGFLDLAAAEPERFYIIDASRSEQEVFAA
                     VKQIVDRRLDLPR"
     gene            5494..6480
                     /gene="holB"
                     /locus_tag="VF182_10675"
     CDS             5494..6480
                     /gene="holB"
                     /locus_tag="VF182_10675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX6437586.1"
                     /translation="MGLSEIIGHEKQIETLRQSLLNGRLHQAYLFIGPDGIGKRTVAL
                     ALAQMIHCMELDSDFCGACGSCRAIQTGNHADFRVVEPLPNKKDISIQQVRELEKALS
                     LRSFSGRQKVAIIDPATSMNWPAQNALLKTLEEPPPGCVLILVASNAGGVLPTVRSRA
                     FALSFAPLPRQLIVRFLISSKGKTREQAEFSAALAMGSLGALSKIDNEKMIENRLEWM
                     ETLVSLRAGDYRAALNAAEALAGNREETLSFLEWAGLWYRDLLTFRIAHAPNQVVNID
                     MLPRIEEQSAGIREDDLFSLLSKTTEASRLIQRNLNRRMVLEDLLLRAVERP"
     gene            6480..8018
                     /gene="metG"
                     /locus_tag="VF182_10680"
     CDS             6480..8018
                     /gene="metG"
                     /locus_tag="VF182_10680"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX6437587.1"
                     /translation="MDRIYITTPLYYVNAEPHLGSAYTMILSDTLARYYRGRGCETFY
                     LTGTDEHGDKIAHAAANAGLEPKEFTDQISATFRSVWDECGFAYDYFIRTTDDYHVRY
                     VQEVLGTVYKSGDIYFDRYSGLYCFGCERFYTDKELVNGKCPDHLTEPELIEEENYFF
                     RMSKYQDHLLRHIERHPQFIRPEGYKNEVLAMLREPIEDLCISRPKTRLTWGIELPFD
                     KRYVTYVWFDALINYVSALKREGEGEFQKFWPEAQHFIGKDIVKPHGVFWPTMLLAAG
                     LPLYKHLNVHGYWTVEGKKMSKSIGNTISPLEVKQTIGMDAFRYFVLRESVFGQDADF
                     RRESLIGRYNADLANNLGNLVSRVLAMQAKYFAGVVQPLSQQWTDEDNQLKNVYAKAE
                     SEIGRHLAELQFHRALEVLWAALDHTNRYIVQTSPFTLMKDPANRPRVGEILHHLLQA
                     VCQLARLLKPFMPDTAKELQSLLAFDEGDPALEASWGNSFAAGHKVNSPKVLFPRIEV
                     PANV"
     gene            8026..8817
                     /locus_tag="VF182_10685"
     CDS             8026..8817
                     /locus_tag="VF182_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013921253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX6437588.1"
                     /translation="MSDPKRLSLVDSHAHIQGTEYAGDVEAVIARAREAGIDKIIAVG
                     GAGEMSSNHEAVALAASYENVYATVGMHPHDAKDVGSEELANLRDLTVHPKVIAVGET
                     GLDYYYSHSPHDVQRRVFAEFIQLARQAGLPLVVHERDAAQDAAQLLRSEGRGNLRGV
                     IHCFTGNYQAACAYLDLGFYLSFTGIITFKNAEPLRDVVRQVPLEKILIETDSPYLTP
                     VPHRGKRNEPAYVRLVAEAVAIMKSISLEEVAQITSRNVRELFSI"
     gene            8901..9377
                     /gene="bfr"
                     /locus_tag="VF182_10690"
     CDS             8901..9377
                     /gene="bfr"
                     /locus_tag="VF182_10690"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEX6437589.1"
                     /translation="MKGNADVLKVLNEVLRKELTGINQYFIHAKMCKNWGYAVLAEHA
                     WNESIDEMKHADQIVERVLFLEGVPNVQAYDKIQVGSNVKQQLENDLGLEQAALTVLR
                     PGIRTCLDARDDASRELLEHIVIDEEHHIDWIETQFHQIKEVGYENYLAQQIFKKN"
     gene            complement(9764..>10793)
                     /locus_tag="VF182_10695"
     CDS             complement(9764..>10793)
                     /locus_tag="VF182_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX6437590.1"
                     /translation="SFDVFLARAQKQMPEWATIMIRLPARGDGPVTVSLQEAQPPHPF
                     ARSQLTLDHTTGEVVKWEPYSASSPGRKLRTYLRGLHTGEALGFIGQTIAGLASLGGC
                     FLVWTGLAMAWRRFRYRERGAQNAMTYTPVPAEIFPAAPINLSQSNETSKTLARIEME
                     LGNCNQTNGRAHNGHEAARPRWMTRYNADSVLILYGTVTGNAESLAHRLAASLRCEGL
                     TSEVRDMAHCQPNVLSDTKCVLMVVSTYGDGEPPDGVIPFWQAVVHGDGPNLSGVRFS
                     VLALGNATFDHFCKCGREFDAALERLGATRIHRRVDCDVDYDAPAQHWLEGVLAQLRD
                     NQHSTLSA"
BASE COUNT         2490 a         2760 c         2909 g         2634 t
ORIGIN      
        1 tcggcaatac ccgaacctca attttgttca agtttgcgcg aaaaaccaaa agacaaatgg
       61 cgcttgcatg gtttgtgtct aatcaaaagt tcaagtcttt tcgtcagcaa ttcggagcaa
      121 cccggtcctg atggatcacg aagcaactgc ttcctcttgt cgcgtcagtg tcattgccaa
      181 tattgtcgtt gatgcataga tgtctgagtc agggcgcact ggccaaccgc gaatctctgc
      241 ttgctcgaag ttacaacggc acaactggaa gtttcgtctt cttggaagtt gttgcgttcg
      301 cccgtagtcc gggcgcatgc tttgcgacct ttgtcatgtc cagcggagtt gattatcaac
      361 tcattcatgc agctttgcag ttcgaagtgt cccagtctga tcaggtctct cgtatgaaca
      421 aacccgagta ctattggatt caaatagaca taagtcgaaa tcttagagta tcccgatgtt
      481 tagcagcagt acatcgcacg ttcactaagc gcgcgggtgt tagctgattt aattacagga
      541 gaagttaaat cgatgatgcc caaatattgt gtcggcttta tcgttttcat tgtcggcgcc
      601 gttgtcggca gtttcttaaa cgtctgcatc tcgcgaatac ccaacggcca atccgtcata
      661 cagccgccct cgcactgccc gaaatgtcaa gcgccgatac cgttctttta caacattcct
      721 ttaatcagct atctactgtt acgcggccgc tgttgttcgt gcggggatcg gatttctttt
      781 cgttactttg ccgttgaact gttgatggca tctctggcgg tgcttttgtt ttaccaattc
      841 ggcttagggg tcgcttttgt tgtagagttc gttttcgttg cggctctgat tgtcatctca
      901 tttatcgact tagatgtaag gattgtgccg gacgtgatca gcttgcccgg gattgtcatt
      961 gggcttctgt tctcggtaat cggccactac ttgattcggg atccgctgca tgttgtgcct
     1021 actccgctga gcgcattcat cggcgtgctg gtgggtggcg gatttcttct ggctgtagct
     1081 tggatttatg agaagtttac cggcgtagag gggatgggcg gcggtgatat taagctccta
     1141 gccatgattg gcgcttttct cgggtggcct tcgattccgt tgactttatt cttttcatcg
     1201 ttaagcggtt ctgtcgtcgg tctaactatc atgttagcca agggggtaga tcgcaggtac
     1261 gctcttccct tcgctccttt cctctgtttg ggcgcgcttg tgtacttatt tttcggtaga
     1321 gagatcatcg attactacgt cccgccccgt tgagtctaac atttcagtct cccacttctt
     1381 cccgttattt ccataaactg cccttggtag ctctgttcgc gacctgacta gcggcccttg
     1441 acagcccctc atgcgcacct gtatggtccg cgaacttccg gaggagccat ggattgccaa
     1501 agcagggtcg gattctctct catcgaactt atcgtggtgc taagtctgat ggggatcttg
     1561 tttgcgattg ggattccggc atcgaacaag ttacttcttt cctatcagct gaacggctcc
     1621 gcgcgccgcg ttcagacgga cttacaaaac attaagatgc gtgcggcagc cgagaacctt
     1681 ggctttcaat tcacatacgg cgcaggaagc ggcagctata ccatccgaag agacggtgcc
     1741 gttttcgtca gcaagccttt acccgaaggc attgtaatca ctcaagccgg taccgtttca
     1801 ttttcaccga gaggcaccgc agggagcaat cgcattcgac ttcaaaatag agggggcgca
     1861 tgcaaacaag tagtggtcag ctccaccggc aggattcggg tctgcaatcc tgctggttgc
     1921 ggcgaggatt gctgaggatg ctgctgcggt tccctgcacg aaaacggtac gaagacggat
     1981 tcacgttcaa cgaagtgtta ctggcgatga acgtgatcgt catcggcatt ctcggctatt
     2041 ccgtcggtac cggtaatctg attcgctgga attccgccag ccgcgattac accgtggcgg
     2101 ttaacctggc gcaagacaag ctggagcaga ttaaagctca gaaaaattgg ccagacgtaa
     2161 acaactgccc ggcatcgggg gaccatggta tcgctgcaac cggtgagccg ggcggtcagt
     2221 ttaaccgatg ttgggtgatt gcggattcga cgttgggagc ggagttgaag gagatcactg
     2281 taacggtttc gtggcccggc cgcgaaagcc gggaagtgac gctcaccacg ttgctttatt
     2341 caggttgaga tcgcctatgc aatttattcg gaacgattgc ggcgccactt tgatcgagca
     2401 actaagtgct ctaatgcttg gtacgataat cattacggcc ttatttggat tctaccgtag
     2461 cgaacttttc catcttctcg cgcaagaaac tcggatggca acactggaag atgccagagg
     2521 cgcaatggac atcattagcc gagatttgaa attggcgggt tcatggggca caggaagcgc
     2581 gccttcggaa actggcttgg gggacgatcc ggacgccgat gacgactctg catgtaatcg
     2641 catttatcgg gcgagcgagc gggtaatcca gattcaaatg gatcttaatg gaaatggtaa
     2701 ttgcgccgat cttgagccgc gcgaaaatat tcgctacgag cttggcgcag ccactacgaa
     2761 ctgcccagga agaacggtca tccgccgcaa tggtgactgc ctggtcgctt atgtgacggc
     2821 gccaaactca ggcaagctgt ttaaatatta cgatgaaaac ggtttgctgc ttagcgatac
     2881 cccgccgctg gcagccatca aacggatcgt gatttcgttc tccgtcggca tgaaggagac
     2941 ggatttcaaa acgggcgcaa gcctcgggtc cgcgctttcc agcagcgttg aactgcggaa
     3001 ctaatgaaag cttcggatgc ctacagcaaa gctctagtag cggaccagcg tgggattgtc
     3061 ctttactctt ctttgatgtt gctggcgctg atcatggctg tgggcgttca ggcgatcgtt
     3121 tcgacgcaaa gtaactttca aatttcagca aatttgcgag ctgaaaacgt cgccttttat
     3181 atatcggaag caggaatcga gtggagcaaa aatgagctcg caaaagccac ggctcatccg
     3241 gtgccgccat tcaattcttc gtcgacaatt tcctcggggg cgttttctgt cgcgacaatt
     3301 tccacgattg cggtctcgcc tttagtgtcc aggatggtgg tacggtcaat ggggaggttt
     3361 gcttcatctt cccagatcgt ccaagccgag ctgatcaagc ggtttctact ggccgacgcc
     3421 gcggtcggat tgcggggaaa tgcaagccac ccgatcttcg tcggcaatga tttctcgatt
     3481 tcgggtatcg atcatgatcc ggcaacaagc aagcccatgg cgagcctgcg atcctatgcc
     3541 ggattcagtg tttcacatcc tgcaactaag gaagcaatcg aggcgtcgct ggaaccgaac
     3601 cagctcagta gagtttccgg tactgatcgt cagggtgcac taatagctga aacccaaaag
     3661 ctgccgtccc cagcaattgc gcagttgact ggcgatctgt gttcagcaga aaatgcccaa
     3721 ctcacagctg ttccggccgg aacttcacta tcattgaccg atcaacagtg gggcgaccga
     3781 tccgtccctc agcttcgctg ccttgatggc gctcccggtt ccgccgatgc ggttgttaaa
     3841 ctcagcaact cgtcaggtgc cggtatcttg atcgctagaa acgctgatct gatactggaa
     3901 ggcgggttcc ggtgggaggg gctgattatc attacgggag acatggtgag tttgaaaata
     3961 ttggaaactg gcaacaaaga gatcatcggc ggggtcatca tcaatgagac cggtactttt
     4021 tccgagacta atccgtcgac actcgaggtt cacggtgata tcaaaatact ttttagccgt
     4081 tccgctttac acactgccgg gaccgtcgtc cccgccagtg ctttgacgac cggtcacgca
     4141 tcattgcccg tggaagtcgt tcagagttat tggagatcga taactcctta gaagtaaatc
     4201 ccggccgatt ctcccgccgt ttgccagcga atccacggaa acggccgcat tacatagcca
     4261 aaacaataat ttacaatctt gggataaggt ggtaaattac ccaatcttaa gcgtcaagcc
     4321 ttgcaactct agggagtaga acgcatggac acacagcgga ccataatggt ggttgacgac
     4381 aatccggata ttattacgat cgtcaaaacc attttggagg ggaagggcta ccacgtgctg
     4441 agtgcttcga gcggtcaaga attattgaat ctgctgacgg atggaaagcc ggacctgatt
     4501 atcctcgata tcatgatgcc tgaaatggac ggattggaag ttctgggccg tctaaaggcg
     4561 gttacagaga ccgcttctat tcctgtaatt ctgctcaccg ccaaggttca atatgaagat
     4621 gttctaggcg ggtataaact aggcgccgat tactacatta ccaagccttt caccagcacg
     4681 cagctagtca atgggatcaa tctgctccta ggcgaaagca agaactagcg ccggcgccgg
     4741 ctccatctcc acctcagcca tttctaccgc tgtattggcg tggtacaaag gtacacgctc
     4801 ggcgaaacag ttctgttgat caatcccatg gttggtttca tcactttcga aggcggtgac
     4861 ggcagcggaa aatctaccca gattagattg ctggagaagc acctcgtgat gcgggataaa
     4921 acctgcctca cgacgcgaga accgggcggc acttcgctcg gtcgacttct gcgccagatt
     4981 cttttagaaa ctggaaaacg gccgatcagc gcctcgaccg agctcttctt gtatttggcc
     5041 gaccgggccc agcatgttca tgaagtgatc gcgcccgcga tccttaaggg tacgatcgtt
     5101 ttgtccgacc gatacataga ctcgaccgtg gcataccagg gctacggacg tggctttgac
     5161 cttcagttgc tgctcagcct gaatcgaatc gccagcgaag gtctgacacc cgatatcacc
     5221 tttttgctcg actgtccggt acagttaggg ttgtcccgta cggtccatcg cgggtcgaat
     5281 ccatcgacat ccaagagtaa cgatcgcttt gaaagcgaga atctggaatt tcacgagaaa
     5341 gtgcgtaacg gctttctcga tttggcggca gcggaaccgg aacgatttta tatcatcgat
     5401 gcttcgcggt cggagcaaga agtattcgca gctgttaaac agattgttga ccgaagacta
     5461 gatttgccgc gctaaaaatt agtagaacca atcatgggcc tttccgaaat cattggtcat
     5521 gaaaagcaaa tagagacgtt gcgccagtca ctcttgaacg gcagattgca tcaagcctat
     5581 ctgtttatcg ggcctgatgg gatcggcaag agaactgttg ccttggcctt ggctcaaatg
     5641 atccactgca tggagttaga cagtgacttt tgcggcgcct gcggttcttg tcgtgccatt
     5701 caaacaggca atcatgccga tttccgagtt gtggaaccac tccccaacaa gaaagatatc
     5761 agcatccagc aggttcgcga attagagaaa gcgttgagcc ttcgatcctt ttcgggacgc
     5821 cagaaagtgg cgatcatcga tcccgcgact tcgatgaact ggcctgcgca aaatgcattg
     5881 ctgaagacat tggaagaacc gccgccgggc tgcgttttaa tcttggtcgc gtctaatgct
     5941 gggggcgtgc tcccgaccgt gcgctcgcgc gccttcgctc tttctttcgc tccattgccc
     6001 cgacaactta tagtcagatt tttaatctcc agcaaaggaa agaccagaga gcaggccgaa
     6061 ttctcagcgg ctttagcgat gggaagcctg ggggcgctaa gcaagatcga taacgaaaag
     6121 atgatcgaaa acaggctcga atggatggag acgctggttt ccttgcgagc cggagactac
     6181 cgggcagcct taaacgcagc cgaagcgctt gccggaaatc gtgaggagac actgagtttt
     6241 ctggaatggg cgggactttg gtaccgcgat ttgctaacgt tccgaatcgc tcatgcgccg
     6301 aatcaagtcg ttaatatcga tatgctgccg cgaatcgaag aacagtccgc aggaatccgg
     6361 gaggatgatc tgttctcgct gttgtcgaag acaaccgagg catcacggct gattcaaaga
     6421 aatcttaatc gccggatggt gctagaagat ctgttgctcc gcgctgtcga gagaccttga
     6481 tggacagaat ttacatcacg acaccccttt attacgtcaa cgcggaacct catttgggca
     6541 gcgcttatac catgatcctt tcggacaccc tggcgcggta ttaccgcggc aggggctgcg
     6601 aaacttttta tctcaccggc accgacgagc atggcgacaa aattgctcac gcggcggcca
     6661 atgcgggact ggagccgaaa gagtttactg atcaaatcag cgcgactttt cgctccgtat
     6721 gggatgaatg cggctttgcg tacgattatt ttatccgcac cacggatgat tatcacgttc
     6781 gttacgtgca ggaagttctg ggcaccgtat ataaatccgg agatatctat ttcgatcggt
     6841 atagcggcct ttactgcttt ggctgtgagc gtttttacac ggataaagag ttggtcaacg
     6901 gtaagtgccc ggaccatctc accgagccgg aactgatcga ggaagaaaat tatttcttcc
     6961 gaatgagcaa gtaccaagac cacctgctgc gtcacattga aaggcatcct cagttcatca
     7021 ggccagaggg atataagaac gaagtgcttg caatgctgcg agaaccaatc gaagacctct
     7081 gcatttcccg ccccaagacc cggctcacgt ggggcatcga gcttcctttc gacaagcgat
     7141 acgtgaccta cgtttggttt gacgcgctga tcaactatgt cagcgctcta aagcgtgaag
     7201 gcgaaggcga gtttcaaaaa ttttggcctg aagcccaaca cttcattggc aaggatatcg
     7261 tcaaacctca tggcgtattc tggcccacga tgcttctggc ggcggggctt ccgctctaca
     7321 agcatctcaa tgttcacggc tattggaccg ttgaaggtaa gaagatgtcg aagagtatcg
     7381 gcaacaccat atcgccgttg gaagtgaagc aaacgattgg catggacgct tttcgctatt
     7441 tcgtccttcg cgagagcgtt ttcggacagg acgccgattt tcgacgagag agcttaatcg
     7501 gacgttacaa tgctgatctg gccaataacc tgggcaactt agtcagtcgt gttcttgcga
     7561 tgcaggcgaa atattttgcc ggtgtggtgc aaccgttaag tcagcaatgg accgatgaag
     7621 ataaccagtt gaagaacgtt tatgccaaag ccgagagcga aatcggacgc catttggctg
     7681 agcttcaatt tcaccgcgca ctagaagttc tctgggccgc tctggaccat accaaccgtt
     7741 acatcgtgca gacttcgccc tttacgctca tgaaagatcc cgccaatcga ccgcgagttg
     7801 gagagatttt gcaccacttg ctacaggcgg tttgccaatt agctcgccta ttgaagcctt
     7861 tcatgccgga taccgcgaaa gaacttcaat cgctgcttgc tttcgatgag ggcgatccag
     7921 cgctcgaagc atcttggggg aattcattcg ctgccggcca taaggttaat agcccgaaag
     7981 tcttattccc tcgcatcgaa gttccagcca acgtctagct tccaaatgag cgatcccaag
     8041 cgtctcagtc ttgtagacag tcacgcccat attcaaggga cggagtacgc gggtgacgtt
     8101 gaagccgtca tcgcgcgcgc gcgtgaggcg ggaatcgaca aaatcatcgc ggtgggcggc
     8161 gcgggtgaga tgtcgagcaa ccatgaggcc gtggcgctag ccgcttcgta tgaaaacgtc
     8221 tatgcaaccg tcggcatgca tccccacgac gcaaaggatg tcgggtcaga agaattagca
     8281 aacttacgag atctgactgt gcatccgaaa gtgatcgcgg tgggggaaac gggtctggac
     8341 tactattaca gtcattcgcc gcatgacgtg cagcggcgag tgttcgccga gtttattcaa
     8401 cttgcgcgcc aggccggctt gccgctggtc gtccacgaac gcgatgctgc gcaggatgct
     8461 gctcaacttc tacgttcgga aggacgtgga aacctgcgcg gcgttattca ttgcttcacc
     8521 gggaattacc aggctgcctg cgcctacttg gatctgggat tttatctttc ctttaccggg
     8581 attattacat ttaaaaatgc cgagcctctg cgcgatgtgg tgcggcaagt gcctttagaa
     8641 aagatactta tcgaaaccga ttcgccttat ctcactccgg tgccgcatcg cggcaaacgc
     8701 aatgagccag catacgttcg cctggtcgcg gaagctgtgg ctataatgaa aagcatttcc
     8761 ctggaagagg tggcacaaat cacatcacgg aacgttcggg agctgttcag catttagcgc
     8821 attgcggcct ttgttgtctt gttcttcgat cattgctgtg tattagattc aaataacact
     8881 gcaagcgggg agggcgaggt atgaaaggga atgccgacgt gctcaaggtg ttgaacgagg
     8941 tcctgcgcaa ggaactcacc ggcatcaatc aatatttcat tcacgcgaaa atgtgcaaaa
     9001 actggggcta tgccgtgctc gccgaacacg cgtggaatga gtcgatcgac gaaatgaaac
     9061 atgcggatca aatcgtcgaa agggttcttt ttctcgaagg cgtcccgaac gttcaagctt
     9121 acgacaagat tcaggtcgga tctaacgtca agcagcaatt agaaaacgat ctcgggttgg
     9181 agcaggcggc gctgactgtt ctcaggcccg gtataagaac ctgcctggac gctcgagacg
     9241 acgcaagccg ggagctgctc gaacatattg tcatcgacga agagcaccat atcgattgga
     9301 tcgagactca gtttcaccag atcaaggaag tcggctacga gaactacctg gcgcagcaga
     9361 tttttaagaa gaattgatcc gcttatcgcg aattttccta atccgaacaa gctgccccct
     9421 tagactcgat cagttagtcc atgtcttgag ggtagatcct gtcccgcaca tctaaatcct
     9481 catcgtggaa tgatcctcac cggccatcgg acctctaact gttggcggtc agggaatatc
     9541 gtattgcgta cgatgatgcc gacggtatct gtggggtatg ggttgaagtt cgtgcgttgc
     9601 cgactggttt tccgttcatt cgcttgacgg gagcggctca aagcgcgcac cgggctcgcc
     9661 agccgacagg gatacattat ctgggtgtac tgagaaatca gccgtcggcc gcaaacgtgg
     9721 tgcatcgaga gaggatccat ttgacttgag cattgtattg actctatgcc gaaagagtac
     9781 tgtgttggtt gtctctgagt tgcgccaata ctccttcgag ccaatgttgg gccggcgcat
     9841 cgtaatcgac atcgcaatcg acacgtcgat ggatgcgcgt agcgccgagc cgttccaacg
     9901 cggcatcgaa ctcccggccg catttgcaga agtgatcgaa cgtcgcgttg ccgagtgcga
     9961 gtacagaaaa cctgacgcca ctcagattgg ggccgtcgcc gtgcaccacc gcttgccaga
    10021 acggtatcac gccgtcgggc ggttcaccat cgccgtaggt gctgactacc atcaagacac
    10081 acttggtgtc actcaacacg tttggctgac agtgcgccat gtctctcact tctgatgtaa
    10141 gtccctcgca tcgcagcgat gccgccagcc tatgagccaa cgactcggcg ttgccggtga
    10201 ccgtgccgta aagaatcaga actgagtcag cgttgtaccg cgtcatccat cgcgggcggg
    10261 cggcttcgtg accattgtga gcacggccgt tagtttgatt gcagtttcca agttccatct
    10321 cgatcctcgc tagggttttt gatgtctcat tactctgcga cagatttatg ggggctgcag
    10381 gaaaaatttc cgcgggcaca ggtgtatagg tcatcgcatt ttgggcgccg cgttcgcgat
    10441 agcggaagcg ccgccacgcc atggcgagtc cggtccagac gagaaaacaa ccgccgagcg
    10501 atgccaggcc ggcaatggtt tggccgataa agcccagcgc ttcgccggtg tggagaccgc
    10561 ggagataagt cctaagtttc cgtcctggac tgctagcgga ataaggctcc cacttgacca
    10621 cttcaccggt tgtgtggtcg agcgttagct gcgaacgcgc gaaaggatgc ggtggttggg
    10681 cctcctgaag tgacaccgtg accggaccgt caccccgggc gggcagccga atcatgattg
    10741 tcgcccactc gggcatttgc ttttgcgcgc gcgcgagaaa aacatcgaag ctc
//
LOCUS       DASUVS010000192        19726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_298033, whole genome shotgun sequence.
ACCESSION   DASUVS010000192 DASUVS010000000
VERSION     DASUVS010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 19726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19726
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298033"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..399
                     /locus_tag="VF182_10700"
     tRNA            324..399
                     /locus_tag="VF182_10700"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Lys,seq:ttt)
     gene            404..481
                     /locus_tag="VF182_10705"
     tRNA            404..481
                     /locus_tag="VF182_10705"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Glu,seq:ttc)
     gene            876..2483
                     /locus_tag="VF182_10710"
     CDS             876..2483
                     /locus_tag="VF182_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX6437591.1"
                     /translation="MGKHPNFLLIITDQQRADHLGCYGNSVVQTPNVDSLATAGTRFD
                     RFYVATPICMPNRATLMTGRMPSLHGARQNGIPLSLSATTFVDILRAAGYETVLIGKC
                     HLQSINGTPPVIGIPQADVKRIAPPEHLREADNSINEQGRYDQELPSTWAHDPEFEPA
                     KPYYGFNHVELAIGHGDKAVGHYERWLKARYSDSDSLRGATNQLPGNDYIAPQAWRTR
                     MPEELYPTTFVAERTMDYLETYYRSDQSRPFFIQCSFPDPHHPFTPPGKYWEMYDPES
                     IALPSSFNSGERPIPPHLQKLYEERELNKSNRDGQRTFAISEREAREAIALTYGMITM
                     IDDAIGKILARLVALGLESNTVIVFTTDHGDFMGDHQLLLKGALHYRGLVRVPFIWHD
                     PAAVKKNIINSGLCGTLDIASTVLDRAGLAGYNGMQGRSLLSAVEGDATGYDALLIEE
                     HQRRGYMGLENNFRARSLITDKHRLTLYEGVEWGELYDLASDPDESNNLWDVSHAAKY
                     RNHLTERLARKMMQLAETSPLAKHHGP"
     gene            2641..2850
                     /locus_tag="VF182_10715"
     CDS             2641..2850
                     /locus_tag="VF182_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HEX6437592.1"
                     /translation="MSEHVYKQIELTGTSKTSVEDAVQNAVTKAGQTLHNIHWFQVIE
                     TRGYVENNKIDYWQVIIKLGFRLDD"
     gene            complement(2937..3758)
                     /locus_tag="VF182_10720"
     CDS             complement(2937..3758)
                     /locus_tag="VF182_10720"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6437593.1"
                     /translation="MTIDPEAFRIFEHAGWETIPERYHLAFGKLTTQTIGPLLDAVRV
                     KAGTKFLDIAAGPGYVAAAAAKRGATVLGLDFSARMLEEARKLHPGIEFREGNAEQLP
                     VGNGLFDSAVSNFGILHFAQPELALREAWRVIRVGGYFAFTAWARPEETIGFDIVLRA
                     VESYGEPRIELPEGPAFFRFSDPAECLRELTGAGFENTTVVKVPQVWRLSDGEALFGA
                     MLNSTVRTAGLLQSQKPVVLQKICAAICADAEQYRIGEVIELPMPALIAAGTKAK"
     gene            complement(3924..4649)
                     /locus_tag="VF182_10725"
     CDS             complement(3924..4649)
                     /locus_tag="VF182_10725"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437594.1"
                     /translation="MVFTSWRGVVGVVKPTHRPGSLEEFIRLLPEGIGVLPVYLNFKR
                     GTVDEFRAALDAVEEKVAELANEGVDLIHPEGAPPFMVHGYQGEKTIITDWECKYKVP
                     IITAAQTQVDALRALNVKKIIGVTYFIGSVNEITTSYFRDAGFDVLAMEGMSVAFEDV
                     GRLASQEIYAHTRQAFLKHPGAEAIYMLGTGWRCLDIIRLLEDDLQVPVIHPVPARVW
                     SVQKRLHVRQPLHGFGRLLLEMP"
     gene            complement(4664..6142)
                     /locus_tag="VF182_10730"
     CDS             complement(4664..6142)
                     /locus_tag="VF182_10730"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6437595.1"
                     /translation="MARPERVGTTQSTVSYHGLRDWLEQVEKLGELQKINGAHWDREM
                     GAITQMLTETGKGKAPAILFDEVPGYPKGYRTLYGQFSTIKRVALTLGLPLEYEHKAD
                     IVKAYHHRMEDMKLLKPKIVKSGPIFDNVLEGDKVDVLKLPVPIHHQQDTHRYIGTAD
                     MCITKDPDAEWYNFGAYRCEVYDEKTIGCQITEGKHGRIHRDKYFERGQSAKFAIVCG
                     QDPLLYLLSASPLPSGVSEYDFAGGIRGAPVEVVKGPFTGFPIPADAEIVIEGEAVPG
                     QVRPEGPFGEWMGYYSDDVVPRPYLNVKTVMYRNDPILTCAPQHKPVDETGLLKGIAG
                     AAEIWKALEACGLPEILGVWNHEGAPATRFTAIQIRQRYPGHARNVLHVAANCMAGAY
                     NGKWTVVVDDDVDCSDMDQVLWAMGTRFDPITDIDLIQKAWSSGRDPMFLPGNFNNRI
                     LIDACVPYNRKLKGEFPKVVEVPNDVRAQLKAKFPQVFKSKS"
     gene            6334..6816
                     /locus_tag="VF182_10735"
     CDS             6334..6816
                     /locus_tag="VF182_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437596.1"
                     /translation="MGSGNGYDQRLRAIGQALDTKRMTAFELKADAERYVVRGVPEKD
                     SSVRARLRDWRNLMQGASKETALSYGTAEIERLDHQGRLNRRKPDRLPDFYSLPNTLR
                     TVGSYLDSKNAELLELHKSPLSVTLLYQNENGHPDMEERSIASFYNLFVALHAKRKSN
                     "
     gene            complement(6893..9121)
                     /locus_tag="VF182_10740"
     CDS             complement(6893..9121)
                     /locus_tag="VF182_10740"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6437597.1"
                     /translation="MTDLRSVGIPSPRVESEQKVGGKAVYAGDVVLPGMLWVKVLRSP
                     VAHARIKKIDMPRALAAPGVKTILAGDDFRGARIGKKIIDMPILAEGVVRYVGEKVAA
                     VAAESEIQAEAALDLIDIDYEELPAVLDPLQAMEPSAPLLHPDLAGYKGLLHKIESPS
                     NLFVHLTWKKGEVEEGFRQSDITVEHTYSVPAVHQAYIEPHGCLVRVNTDGRAEIWAS
                     TKSPFNLREQVGNALQVPAASLIVHPCYVGGDFGGKGDANEVALCYALAKKTGRPVKL
                     LIDYSEELLAGNPRHGAVIRVKTGVKMNGMMMAQRIEFVFDSGAYGSYRPQGYLVGAH
                     DAAGPYRVANCFIEEKYVYTNKTPCGYMRAPGHLQAFFATESHADLVARRLEMDPAEY
                     RRINFMTDGDKSPLGEVMPHVKPGDTLLKAVEISDYRRPKRKNVGRGCAIANWVSKGG
                     ESYAFVRIDKDGVVTLSSAVTDTGPGAFTIMRQIVGQELKVSPESIRVEMLDTTQVIK
                     DTGVRGSSSTRVHGGSAYEAAKKASEEVLRIAAEAMNAAPEELILYDGGVTHGRAERR
                     MTFAEIAEANEGSIIGQGHYANMADGPETSIVAQVAEVEVDTQTGEVQLIKLTTAHNT
                     GAILNPLTHQGQIDGGVIMGMGYGIMEDLTVDESGKVLTTNLGEYKIPNIKDVPVLKT
                     AIIQSAIGSGPYNSMSIGETALIPTAAAIANAVDDAVGARITSLPITAEKVLNAIRGR
                     SE"
     gene            complement(9422..13792)
                     /locus_tag="VF182_10745"
     CDS             complement(9422..13792)
                     /locus_tag="VF182_10745"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6437598.1"
                     /translation="MDAFHSLIQRWFRERFENPTLPQQEGWLSIARGQPTLIAAPTGS
                     GKTLAAFLWSIDRLFKRAISGELRDQTSVVYVSPLKALGNDIAKNLQQPLDEIFHLAY
                     REGIMLPEIRTAVRSGDTPARERQLMVRRPPHILITTPESLYILLSSPRSREFLKNAE
                     TVIIDEIHAIAADKRGAHLALSLERLNQLAERPLQRIGLSATQKPIEEIARLLVGSRA
                     VHEDGSPDCVIVDAGHQRHLELRIEVPDEELGPIIRQDIWAGVYDKIVEQIKNHKTTL
                     VFVNTRRLVERVAHQLTERLGEGKVGAHHGSLSRKTRLEVEQKLKMAQYPVVVATASL
                     ELGIDIGHVDLVCHIGGPRTLAVLLQRVGRSGHWLGATPKGIFYPLTRDDLMQCAAAI
                     RSVRQGELDKIALIEKPLDILAQQMVATVASMTPAVPADISASKSQPEGGINEERLWH
                     MVRGAYPYRNLSWVEYEQMLHMLSEGVETRRSRRGAYIHRDRIHGMLRARRGARLTAI
                     TNGGAIPDTADYDVVEFPSETMVGKVNEDFAIESLTGDIFLLGNRSWRIRRVGSGKVW
                     VEDAQGLPPSIPFWLGESPGRTVELSKAVSDLRRDVADRISDRTGAQGWLVGEINIPE
                     AAADQMVNYVAETQSVLGTMPGQQCIIAERFFDEGGGMQLVIHSPWGARINRAWGLAL
                     RKRFCVSFDRELQAAATDDGIVISLVEQHSFPLSDVFQMLRPAMIEPSLIQAALASPM
                     FTNRWRWNSTRALAVMRYSGGRKVPVALQRIRAEDLLAAVFPEQMVCGDNRTGPVELP
                     DHPLVNETIRDCLHEAMDLDGVKKIIAAIESGAINTLAVDTPAPSPMAHEILNANPYA
                     FLDDAPLEERRARAVSLRRTDLRLEREFGQLDQAAIDEVCHQAWPEIRNGDELHDFLL
                     GVGILPVQERPEWRRIAEELINNGRVTEATWLPPGSQSAITAYVAAERFEWVRTCLPD
                     ATLAPTVEFPSGFEAQALSEEEAFRRIVQGWMEAIGPTTVNALSKRLGIEARKIESAL
                     LSSEGTGMVLRGRFTPSSDSAGEIEWCDRVLLARIHRLTLGRMRREIEPVSPVEFMNF
                     LLSWQHVGVNRQLEGRDGVLRVIQQLQGLELPAPAWEQSVLPARVRNYSPADLEHLCL
                     AGVVAWGRLRPEQSADDHLSEKIRTRRRRKRLAAPARNAPIAFLLRDDLDYFLRDRSL
                     SWVDVATLSSSARDVARYLEQRGASFVADIARGAGLLKIKVEEALWELVAHGLATGDG
                     IAGLRVLLLPEHKRQGKRHNFRVISGGKSAVRTMPVGRWSLWNNQNGDAQLNREQIIE
                     RQARQLLERYGVVFRDLLAREANLPPWRTLLTTYRRLEARGEIRGGRFVNGFVGEQFA
                     LPVAIEKLRAERRSDDSQQPFIISAADPLNLVGILTPGGRVSPYSNQAIAYHKGLPVQ
                     VGPLGSVLSSLQIHQAGEKP"
     gene            complement(13873..14214)
                     /locus_tag="VF182_10750"
     CDS             complement(13873..14214)
                     /locus_tag="VF182_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437599.1"
                     /translation="MDEERNEIEEPEPAAPRVERGANLPLTILLGALVLWFGFQTVQL
                     ASERANLGEARGHQEAAMQEAQKLRTQFESLISKTSELANKGHAGAKLVMEELQKRGM
                     GTQPESITPQK"
     gene            complement(14226..14516)
                     /locus_tag="VF182_10755"
     CDS             complement(14226..14516)
                     /locus_tag="VF182_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437600.1"
                     /translation="MKTGKMVLTGLSVLVLLLVIVNILLMAGNQSLQLSVNERQQYIA
                     ETIQLEALNRQVIGVLAEMALKTNDGQLKELLSGVGINLSPTPSQPPAANPK"
     gene            complement(14572..15222)
                     /gene="lepB"
                     /locus_tag="VF182_10760"
     CDS             complement(14572..15222)
                     /gene="lepB"
                     /locus_tag="VF182_10760"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX6437601.1"
                     /translation="MSAGKVTKAESQSLAKSKSTFREYAEAIGMALLLALFIRTFIVQ
                     AFKIPSGSMIPTLQIGDHILVNKLAYGIRVPFLEDYVVDFSKPKRGDVIVFIFPEDRS
                     KDFIKRVIGVAGDVVQIRGKKISINGKEIDDPHAHFEGDDPQTAGLSNRDDYGPRTVP
                     ENHIFVMGDNRDRSYDSRFWGYVNLDEVRGKAFLIYWSWDGTDRWVRWERLGSLIR"
     gene            complement(15234..17027)
                     /gene="lepA"
                     /locus_tag="VF182_10765"
     CDS             complement(15234..17027)
                     /gene="lepA"
                     /locus_tag="VF182_10765"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX6437602.1"
                     /translation="MSLEHIRNFSIIAHIDHGKSTLADRLLEFTGAVSAREKTDQILD
                     SMDLERERGITIKASPVCLRYRAKNGKDYKLNLIDTPGHVDFTYEVSRSLAACEGALL
                     IVDAAQGVEAQTVANVHMALDHDLEILPVINKIDLPSADPERVRKDIEDVIGLDASNA
                     ILASAKEGVGTEEILEGIVQRFPHPKGTVDDPLRALIIDSWFDPYHGAVVMMRVFDGK
                     VAKGSRIRMMFSGRAFEVTRLGVFAPAPVEVEELAAGEVGFLMAAIKEVAETRIGDTI
                     TLDDRPAGEALAGFKPMKPMVFSGLYPTDANQYQGLREALDKLRLNDSSFTFEPETSQ
                     ALGFGFRCGFLGLLHMDIIRERLEREFGLSLITTAATVVYRVITTSGEALHIDNPVRL
                     PPEGDIEQIEEPIIQATIHLPQDFLGGVLGLCEEKRGTQKEIRYLGPKRVMIVYELPL
                     NEIVVDFYDRLKSVSRGYASMEYELAGYRAADLVKLDVRINGDVVDAMSMIVHRDKAY
                     YRGRDLVQKMRELIPRQMFEVVIQAAIGSRIISREAVKALRKNVTAKCYGGDITRKRK
                     LLERQKEGKKRMKQVGKVEIPQEAFLAALKV"
     gene            complement(17024..17950)
                     /locus_tag="VF182_10770"
     CDS             complement(17024..17950)
                     /locus_tag="VF182_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6437603.1"
                     /translation="MKIVVTGGAGFIASHIVDAYLHQGHEVHVIDDFSTGRKLNLNPN
                     ALLHKFNVADAEAARMIQKIKPDVLNHHAAQMDVRHSVEDPLFDARVNIVGFLNLLEA
                     CKKAGTAQVVFASSGGAIYGDEEPIPADEDHPKKPMSPYGVSKMTGELYLAYYYMAFG
                     MRYVALRYANVYGPRQSTKGEAGVVAIFISQLLAGKPPTINGDGKQTRDYVFVGDVVR
                     ANIAALETGHVGGINIGTGRETDVVTLCRMLQERSRSKVEAVHGPAKTGEQMRSSLEN
                     GLALRVLNWRPERELEEGLTQTIDYYLQNEIS"
     gene            complement(17947..18891)
                     /locus_tag="VF182_10775"
     CDS             complement(17947..18891)
                     /locus_tag="VF182_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEX6437604.1"
                     /translation="MAKYLITGGAGFIGSHLCENFLSQGHDVICMDNYSTGAQQNIAS
                     FAANRRFTFMDHNVSRYIEVQQPLDFVLHFASPASPVDYLELPIPTLKVGSLGTHNAL
                     GLAKAKNAVFLLASTSEVYGDPLVRPQHEEYWGNVNPVGPRGVYDEAKRFAEAMTMAY
                     HRYHGLDTRIVRIFNTYGPRMRMQDGRVVPNFILQALKGEPLTVYGQGEQTRSFQYVD
                     DLVAGIGRLLESCEHYPVNIGNPHEMTVLQFAKKILQLTGSRSEISYRPLPQDDPQVR
                     QPDITKAKKLLNWEPKVNLDEGLKKTIDYFRARLKGQP"
     gene            complement(18884..>19726)
                     /locus_tag="VF182_10780"
     CDS             complement(18884..>19726)
                     /locus_tag="VF182_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX6437605.1"
                     /translation="AAVEDFMKPDRVVLGGDDPEALETVKELYEPFLRTGNQILVMDS
                     RSAEMSKYAANAMLATKISFINEVSRLCEYMGADINDVRRAIALDRRIGQHFIFPGVG
                     YGGSCFPKDIRAIISMGGDQEEMSLMKAVEQVNERQKRVLVEKVKRRFGAKLSGKTFA
                     IWGLAFKPRTDDMRDAPAIVVIEALLQAGARIQAFDPEATEEARKIFRDRVVYAARNY
                     DALDGASALLVLTEWNEFRRPDFERIKELLKEPIIFDGRNIYDPGELKKLGFDYYSIG
                     RTNG"
BASE COUNT         4341 a         5646 c         5209 g         4530 t
ORIGIN      
        1 cttgcggata tcgtagaaag ctgcgcaaga cagcttgaag ctcacggatt cattcgagtc
       61 gatgaatcat ttgttccgcc gtcccccgct ctcaaaagca caccggcttt gaacgaacag
      121 tcaccgcgct aagtaagcag atcaatcggg ttccgctcgg aggccatgca cgaggttctc
      181 tcatttcgaa tgcgcaaagt taaattcgtg gcccacctta gttgtaataa gacccaaaac
      241 cacgatcttg cctttcgttg tcttccaagt tcattgactt agtaagccaa tgtcgctatc
      301 aattaacgag tttacccgcc agcgagccgt tagctcaggc ggtagagcat ctgcctttta
      361 agcagagggt cgctggttcg aatccagcac ggctcaccac tacgtccccg tcgtctagcc
      421 tggcccagga caccggcctt tcacgccggc gacacgggtt caaatcccgt cggggacgcc
      481 aatgatgatc ttgttgttca gcgtttcgtg tccctcacca tctaatcgat taaatgtcat
      541 gctctaccgt caagtaaagc acatcgaacc aatcgaccct ggaagtctta gcaccttctg
      601 atgatcgaag cgctgtcgcg gcgccctgca aactaatcca ggccaaatct ggttaagccg
      661 cgaccgaggg gattcacaca atgaggcaaa gtaaatcaag caaatcgatc gtgctactct
      721 acgatcgtgg catcgcttca gctctggccg accagactct tgtaccggca acatcggtta
      781 tattcgagat ctttcacaga gctatatatc cttatgaaag acgtttcgag ccagttgctc
      841 gtgtgatact tacctataaa aggatgatac taccaatggg caagcaccct aattttctgc
      901 tgatcatcac cgaccagcaa cgagccgatc acttgggttg ttatggcaat tctgtggtgc
      961 agactccaaa cgtcgattcg ttggcaacgg caggcacacg gttcgatcgt ttttacgtgg
     1021 caactccgat ttgcatgccg aatcgagcca cgctgatgac cggtcggatg ccgtcgctgc
     1081 acggggcgcg tcagaatggc attccacttt cactgagcgc gaccacgttt gtcgatattt
     1141 tgcgcgctgc gggctacgag acggttctga ttggaaagtg tcatctccag agcataaacg
     1201 gcactcctcc ggtcataggc atacctcaag cagacgtaaa gcggattgcg ccgcccgaac
     1261 atttgcgtga agccgataac tcgatcaatg aacaaggccg ctacgatcag gaactaccat
     1321 cgacctgggc gcacgatcct gaatttgagc cggcgaagcc ctattacggt ttcaaccatg
     1381 tggaactggc gatcgggcac ggcgataagg ctgtcggaca ttatgaacgt tggcttaaag
     1441 cacgttattc ggattccgac tccttgcgcg gagcgacgaa tcagcttccc ggaaacgatt
     1501 atattgcacc gcaggcttgg cgcacgagaa tgccggaaga actctatccg accacttttg
     1561 tcgcggaacg cactatggat tacttggaga catattaccg ctcggatcag agtcggccat
     1621 ttttcattca gtgttcgttt cccgacccgc accatccatt cacgcctccg ggcaaatact
     1681 gggaaatgta tgatccggaa tcgatcgcat tgccgtcgtc gtttaattca ggagaaaggc
     1741 ctatcccgcc gcacctgcaa aaactttatg aagaacgaga gttgaacaag agcaatcgtg
     1801 acggtcagcg aactttcgca atcagcgagc gcgaggcgcg tgaggcaatc gcgcttactt
     1861 acggcatgat caccatgatc gatgacgcga tcggcaagat actcgcgcgg ctggtggcgc
     1921 tggggctgga gtcaaatacc gtgatcgtgt tcaccaccga tcatggggat tttatgggcg
     1981 atcaccaact acttttgaaa ggcgcgctgc actaccgcgg gctagttcgg gtgccattta
     2041 tctggcatga tcctgccgca gtcaaaaaga acatcatcaa ttccggactg tgcggcacgt
     2101 tggacatagc cagcactgtt ctcgaccggg ccggcttggc aggatacaac gggatgcagg
     2161 gacgaagttt gctttccgcc gtggaagggg acgctacggg gtacgacgcg ttgctcatcg
     2221 aggaacatca acgacgcggc tacatgggtc tggagaataa tttccgggcg cgaagtctga
     2281 tcaccgataa acatcgactt accttgtatg aaggagtgga atggggcgag ctgtatgatc
     2341 tggcgagcga tccggacgag tcgaacaact tatgggatgt ttcgcatgcc gctaaatatc
     2401 gcaaccactt aaccgagaga ctcgcgcgta agatgatgca gttggccgag acaagcccgt
     2461 tggcgaagca tcacggcccc tgatcaaggt ggaagccacc ttggttcttg caacatagtt
     2521 cgttatcttt gctgtgacat ccgtgggaca ggcatccagg aggacccctt aaaaattagc
     2581 ttttgaagcg ggcaccttta ttgcttttcg accgaaaacg accaaacctc gaggtgaaac
     2641 atgagcgagc acgtctacaa acaaatcgag ttgaccggca cctccaagac cagcgtcgaa
     2701 gatgcggtac agaatgcagt tacaaaggcc ggccagacgc tgcacaacat tcattggttc
     2761 caggtaatcg agacgagagg ctacgttgag aacaataaga tcgattactg gcaagtgatc
     2821 ataaagcttg gcttccggct ggacgactga gccctcggtt aaggtagcgc ggttgccatc
     2881 ggcgatttga aagtcgaacc gccggtagag tcgtccacca acgttaagcg ccggtcttac
     2941 ttcgctttgg tccctgcggc gatgagcgcc ggcatcggca gctctataac ctctccgatt
     3001 cggtactgct ccgcatcggc gcagatcgct gcacaaatct tttgcaatac caccggcttc
     3061 tggctctgga gcaatccggc tgtgcgaacc gtactattta acatagcacc gaaaagcgcc
     3121 tctccatctg acaagcgcca tacttgagga actttgacga cggtcgtatt ctcgaagcct
     3181 gcgccggtca gttcccggag acattcagca ggatcgctaa agcgaaagaa ggctggtcct
     3241 tccggcagtt cgattcttgg ttcgccgtag gattccaccg ctcgaagcac gatatcgaac
     3301 ccgatggtct cctcaggtcg agcccaggcg gtaaaagcaa aataaccgcc aactcggata
     3361 actcgccagg cctcacgtag cgcgagctcc ggctgggcga agtgaaggat accaaaatta
     3421 cttacggccg aatcgaatag tccgttgcct actggtagct gctccgcgtt cccctctcgg
     3481 aactcgatgc cgggatgaag cttgcgcgcc tcttctagca tcctcgccga gaaatccaga
     3541 ccgagcacgg tcgcgccgcg ttttgctgcg gctgcagcga catagccggg ccctgcggcg
     3601 atgtcgagga attttgtgcc ggcctttacc cgaaccgcat ccaacagagg tccgatcgtt
     3661 tgggtcgtca gcttgccgaa tgccagatga taccgctcgg gaatcgtttc ccatccggcg
     3721 tgttcaaaaa tccgaaacgc ttcggggtcg atcgtcatag gactgggcca tgcaaaacga
     3781 acttaacagt ttgtcggttt ggaaggaggt ttttcgacgt tgtgcggtga ttgacgtcgt
     3841 cactgttcaa gccgcgctca actcacgtga cttctggatg agactacgag gagtatcatt
     3901 ggattgagat tcttaggatt gaattacggc atctcaagaa gtaaacgccc gaaaccgtgc
     3961 aatggttgtc ttacatgcag ccgtttctgc actgaccaga ctcgggcggg caccggatga
     4021 atcaccggaa cctgcaaatc gtcttccaac aaacgaatga tgtccaggca gcgccaacca
     4081 gtgcctagca tgtaaatcgc ttcggcaccg ggatgcttta agaaggcttg tcgcgtatgc
     4141 gcgtagattt cctgcgacgc gagccggccg acatcttcga aggcaaccga catcccttcc
     4201 atggccagca catcgaaacc ggcatcgcgg aaataactgg tcgtaatctc gtttacgctg
     4261 ccgataaagt aggtgacacc aataatcttc tttacattta aagcacgtaa ggcgtctact
     4321 tgggtttgcg ccgccgtaat gatcggcacc ttatatttac actcccagtc tgtgataatc
     4381 gttttttcgc cttgataacc atgcaccatg aatggtggag cgccttcggg gtgaatcaga
     4441 tcgacgcctt cgttggccag ctcggcaact ttctcttcga ccgcatcgag ggcggcccgg
     4501 aattcatcta ccgttccccg cttgaaattt agataaaccg gcaggacgcc gataccctcg
     4561 ggcagcaaac gaatgaattc ttcaagtgat ccgggacggt gtgtcggttt cacgacaccg
     4621 acaacgccgc gccagcttgt aaaaaccact ccgcgctcct tcgctacgat tttgacttga
     4681 atacctgcgg aaacttcgcc tttagctgcg cccgcacatc attggggact tccaccacct
     4741 tcgggaattc ccccttcagc ttgcggttat agggaacgca cgcatcgatc aaaatgcggt
     4801 tgttaaaatt tcccggcaga aacatcggat cgcgccccga agaccaggct ttctgaatca
     4861 gatcgatatc ggtgatcgga tcgaaccggg tgcccatcgc ccatagcacc tgatccatgt
     4921 ccgagcaatc gacatcatcg tcgacaacca ccgtccattt gccgttgtac gcgccggcca
     4981 tacagttggc ggcgacgtga agcacgttgc gagcatgccc cggataacgt tgccggatct
     5041 gaatggcggt gaagcgagtc gccggggcgc cttcgtgatt ccagacgccc aaaatctccg
     5101 gcaacccgca ggcttccagc gctttccaaa tttccgcagc gccggcaatg cctttgagca
     5161 agccggtttc gtccaccggc ttgtgctgtg gcgcgcatgt gagaatcgga tcattgcgat
     5221 acatgacggt tttcacattc aagtacggtc gcggcacgac atcgtcggaa tagtagccca
     5281 tccattcgcc gaacggtccc tcaggtctga cctgccccgg caccgcctcg ccttcgatga
     5341 cgatctccgc gtcagccgga atcggaaaac ctgtgaaggg tcctttcacg acctccaccg
     5401 gcgcgccgcg aatccctccc gcaaaatcgt attcagagac tccgctcggc aatggactcg
     5461 ccgagagcaa gtaaaggagc ggatcctggc cgcagacaat cgcaaatttg gcgctctgcc
     5521 cgcgttcgaa atacttgtcg cggtgaatgc gtccgtgttt accttcagta atctgacaac
     5581 cgatagtttt ttcatcgtaa acttcacagc gataggcgcc aaagttatac cattccgcat
     5641 ccggatcctt ggtgatgcac atatcggcag tgccgatgta gcggtgcgta tcttgttggt
     5701 gatgaatcgg caccggcaac ttgagcacat ccaccttgtc gccttccaac acattgtcaa
     5761 atatcggccc gcttttgacg atcttcggct tgagcagctt catgtcttcc atgcgatgat
     5821 gataggcttt gacaatatcg gccttgtgct cgtattcgag cggcaacccc agagtcaagg
     5881 cgacccgttt gatggttgag aactgtccgt agagcgtccg ataacctttt ggataaccgg
     5941 gaacttcatc aaaaagaatc gccggcgcct tgcccttgcc ggtttccgta agcatctggg
     6001 taatcgcccc catttctcga tcccagtgcg cgccgttgat tttctgcaac tcgcctaact
     6061 tttcgacctg ttccagccag tcgcgcaaac catgatagga caccgtgctt tgggttgttc
     6121 caactctctc tggacgtgcc attgatcctc ctaattattt gctcagtgtg ggttgtgccg
     6181 gtgcatcggg gagcacaaga aaccatacgt ttggctcccc ttcttgtcaa ggaactcagg
     6241 cgcttagctc ttgtttaccg attttaccga tgatatgacg cggagcgttt ctgataatcg
     6301 gtgataaagg cctgctcgga aagtgagggt ttgatgggat ctggaaatgg ttatgatcaa
     6361 cgtcttcgtg cgattgggca agcgctcgac accaagcgga tgaccgcttt cgaactcaag
     6421 gctgatgccg agcgctatgt ggtgcgcgga gttccggaaa aggattcaag cgtgcgagcg
     6481 agattgcgcg attggcgtaa tctcatgcag ggggcatcca aggaaaccgc gctttcctat
     6541 ggaaccgccg agatcgaacg cctcgaccat caaggccgtt tgaaccgaag gaagcctgac
     6601 cggttgcccg acttttatag cttgcccaat actttgagaa cggtgggatc ttacctggat
     6661 tcgaagaacg cggagctgct ggaattgcac aaaagtccgc ttagcgtcac tctcttatac
     6721 caaaatgaaa acggccatcc cgatatggaa gaaagatcga ttgcgtcatt ttacaactta
     6781 tttgtcgcgt tacacgctaa gcgcaagagc aactgacagc gtcggtgcgt tgggcaatag
     6841 atgacggacg ccggcgcgtt tcagttagag attgttttct cacaaaattc tgctactccg
     6901 agcggcctct gatcgcgttc agcactttct cagcggtaat cggcaaagac gtgatgcgcg
     6961 cgcccaccgc gtcgtccacg gcgttggcaa tggcggcggc ggtgggtatc aatgcagttt
     7021 cgccgatgct catactgtta tagggaccgc tgccgattgc cgactgaatg atagcagttt
     7081 tgagcaccgg cacgtctttg atattaggga ttttgtattc tcccaggttg gttgtcagaa
     7141 cctttccgct ttcatcgaca gtgagatctt ccatgatgcc atagcccatt cccataatca
     7201 cgccaccgtc gatttgcccc tggtgggtta acgggttaag aatggctccg gtattatgag
     7261 cggtggtcag ctttataagc tggacctcac cggtttgagt atccacctcc acctcggcca
     7321 cctgagcgac aatcgatgtc tccggcccat ccgccatatt ggcgtagtgc ccctgcccga
     7381 ttatggaacc ctcgttagct tcggcaatct ccgcaaaggt catgcgccgt tcagcgcgac
     7441 catgagtgac gccgccatcg taaagaatca gttcctccgg cgctgcattc attgcctcgg
     7501 cggcaattct caggacctct tcgctcgcct ttttagcggc ctcatacgcc gatccgccat
     7561 gcactcgggt gctgctactg ccgcgcactc cagtatcttt gatcacttga gtcgtatcca
     7621 gcatctcgac ccgaatcgac tccggggata ccttgagctc ttggccgacg atttgacgca
     7681 tgattgtaaa tgcgccagga ccggtatcgg tcaccgccga cgaaagtgtg acaactccgt
     7741 ctttatcgat tctgacgaaa gcgtaggact ctccgccttt ggaaacccaa ttggcaatcg
     7801 cgcagccgcg gccaacgttc ttgcgcttgg gacggcggta atcggatatc tcgaccgcct
     7861 tgagcaaggt atcgccgggc ttgacgtgcg gcatgacttc acccaatgga gatttatcac
     7921 cgtcggtcat gaaattgatt cggcgatact cggctggatc catttccaga cgcctggcaa
     7981 cgaggtcggc atgactttcg gtggcgaaaa atgcttgtag atgtccggga gcgcgcatgt
     8041 aaccgcatgg cgtcttgttg gtgtagacgt atttttcctc gataaaacaa ttggccaccc
     8101 gataaggtcc tgcggcatcg tgagcgccga ccagatatcc ctgcgggcga tacgagccgt
     8161 aggcgccact gtcgaagacg aattctatgc gctgagccat catcatgccg ttcatcttga
     8221 caccggtttt gacccttatc acggcgccat ggcgcggatt tccggcgagt aattcctcgc
     8281 tgtaatcgat cagcagcttg accggtcgcc cggtcttttt tgccaatgca taacaaaggg
     8341 cgacttcatt ggcgtctccc ttgcccccga aatcgccgcc gacatagcag ggatgcacga
     8401 tcaagcttgc cgccgggact tgtaacgcat tgccgacttg ctcgcgcaaa ttaaaagggc
     8461 tcttggtcga agcccagatt tctgccctac catcggtatt cactctcacc aaacaaccat
     8521 gaggctcgat gtaggcctga tgtacggctg gaaccgaata cgtgtgctcg acggtaatgt
     8581 cggactgccg gaaaccttcc tccacttccc ccttcttcca agtgagatgc acgaaaagat
     8641 tgctcggcga ttcgatctta tgtagcaggc ccttatatcc tgctaaatcc ggatgcagca
     8701 ggggtgccga aggttccatc gcttgaagcg gatcaaggac agccgggagc tcttcgtagt
     8761 cgatatcgat caaatccagc gccgcttccg cctgtatttc agattcagcc gccaccgctg
     8821 caaccttttc accgacgtaa cggacgacgc cttccgccag gatcggcata tcgataattt
     8881 tcttgccgat cctggcgccg cggaaatcat ctcccgcgag tatcgtttta acgccgggtg
     8941 ccgcgagggc tcgcggcata tcgatttttt ttatgcgcgc gtgagcgacc gggcttctca
     9001 gcactttcac ccatagcatg ccgggaagaa ccacatcgcc ggcataaacc gccttgcccc
     9061 cgactttttg ttcgctttca acccgcgggc tagggatgcc aacgctacgt agatcggtca
     9121 taagcaacct cgattgaatt ttcttgaggg tcaccacttg aagatcggtg ctagctatga
     9181 gtctggttaa cttcgattct tagcgcgctg aatattgagc tgcgtttttc agtgaccata
     9241 ggttcagcag tggattcaag tcaacactaa agctaacact gagcggctct aaaaaggacg
     9301 tcaagctccg aaggaaggtt gccagagtcc gccaatgacc tcgtcttagc gcttctaaaa
     9361 gaaagagaga gttgtgactg tgagttctgt ccaaactcta cacgccgaag ctcctataaa
     9421 ttcacggctt ttctccggcc tgatgtattt gcaagctact gaggacgctg ccgagaggcc
     9481 cgacttgaac cggcaggcct ttgtggtagg cgatcgcctg attcgaatag ggggaaaccc
     9541 gtccaccggg cgtaaggatt cccaccagat tcagggggtc cgctgccgat atgatgaacg
     9601 gctgctgact gtcatcactg cgccgttcgg cacgaagctt ctcgatggca accggcaacg
     9661 caaattgctc gccgacgaaa ccgtttacaa agcggccgcc gcgaatctcg ccgcgcgcct
     9721 ctaatcgacg ataggtcgtg agcagagttc gccatggcgg gagatttgcc tcccgggcga
     9781 gaagatcacg aaaaaccacg ccatatcgtt cgagtagttg ccgcgcctgc cgctcgataa
     9841 tttgttcgcg gttcaattga gcgtcgccgt tctgattatt ccacagggac cacctgccta
     9901 cgggcatcgt ccgcaccgcg ctctttcccc cactaatcac cctgaaattg tgacgcttcc
     9961 cctggcgctt gtgctcaggc agcaacagca ctcgtaaccc ggcaatgccg tcgccggtag
    10021 ccagaccatg ggccaccagc tcccaaagcg cttcttcgac cttgattttc agaaggccgg
    10081 cgccacgcgc aatgtcggca acaaaggacg cgccccgctg ttccagatag cgcgccacgt
    10141 cgcgcgcgct gctcgaaagc gtggccacat cgacccaaga cagtgagcgg tcccgaagga
    10201 aataatccag gtcgtcccgc agcaagaaag cgatgggagc gtttcgcgcc ggagctgcca
    10261 agcgcttgcg ccggcgcctg gtgcgaattt tttccgaaag atgatcgtcg gcactttgct
    10321 cgggccgcaa tctgccccaa gccacgacac cggcaagaca cagatgttcc agatccgctg
    10381 gggagtaatt ccgcacgcgc gccgggagaa ctgattgttc ccaggcaggc gccggcagtt
    10441 ccagtccctg caactgttgg atcacgcgca ggactccgtc acgcccctcg agctggcgat
    10501 tgactccgac gtgctgccag gacaatagaa aattcataaa ttctaccggc gagaccggct
    10561 ctatctctcg gcgcatgcgg ccgagagtga gacgatggat gcgcgccagc aagacgcgat
    10621 cacaccattc gatttcaccg gccgaatcgc tcgatggagt gaaccggccg cgcaaaacca
    10681 tcccggtccc ttctgaggac aacaacgccg actctatctt gcgcgcttcg atacccagac
    10741 gcttcgacaa ggcgtttacg gtcgtcggtc cgatcgcttc catccagccc tggactattc
    10801 ttctgaacgc ttcttcttcc gacagggctt gcgcttcgaa tccggacgga aactctacgg
    10861 ttggagcgag cgtcgcatcc ggcaggcaag tccggaccca ctcaaacctt tcggccgcga
    10921 catacgcggt gattgcgctt tgagagccag gaggcagcca agtcgcttct gtaacgcggc
    10981 cgttgtttat caactcttca gcgatcctgc gccactcggg gcgctcctgc accggcagaa
    11041 tgccaacccc gagcaaaaaa tcgtggagct cgtcgccatt gcggatttcg ggccaggcct
    11101 gatggcagac ctcatcgatt gccgcttggt caagctgccc gaattcgcgc tccagccgta
    11161 gatccgtccg tcttagagaa accgcccggg cgcgccgttc ttccaggggt gcatcgtcca
    11221 ggaaggcata aggattagca ttgaggatct catgtgccat gggcgatggc gcaggcgtat
    11281 ccacagccag tgtgttgatc gcaccacttt cgatggctgc gatgatcttt ttgacaccgt
    11341 cgagatccat cgcttcgtgc aggcaatcgc ggatcgtttc attgaccagc gggtgatccg
    11401 gcaactcgac cggaccggtt cggttgtcgc cgcacaccat ctgctcggga aaaaccgccg
    11461 ccagcagatc ttcggcgcgt atccgctgca acgccaccgg cacttttctg ccgccgctgt
    11521 aacgcatcac agccagcgct cgggtggaat tccagcgcca gcggttagta aacatcggcg
    11581 aagccaaggc cgcctggatc aggcttggct caatcatcgc cggccgcagc atctgaaata
    11641 catcgctcaa cgggaaggag tgttgctcga ccagagagat aacgatgccg tcatcggtgg
    11701 cggccgcttg caattcgcga tcgaaactga cgcagaagcg ctttctcagc gccaaacccc
    11761 aggcgcgatt gatacgcgcg ccccaggggg agtgaatcac cagttgcatg ccgccgcctt
    11821 cgtcaaaaaa ccgctcggcg ataatgcatt gctgcccagg catagttccc agcacgctct
    11881 gagtttccgc tacatagtta accatctggt ctgcggccgc ttcagggata ttgatttcac
    11941 cgacaagcca gccctgagct cccgttcgat ctgaaatccg gtcagccacg tcgcggcgta
    12001 aatccgatac cgctttggat aattctacgg tgcgccccgg cgactcgccg agccagaagg
    12061 gaatactcgg cggcagcccc tgcgcatctt cgacccaaac cttgcccgag ccgacccgtc
    12121 ggatgcgcca agatcgattg cctaataaga aaatatctcc ggtgagactt tcgatggcaa
    12181 aatcttcatt gactttgccg accatcgttt cgctgggaaa ctcgaccacg tcgtaatcag
    12241 cggtatcggg gattgctccg ccattggtga ttgccgtcaa ccgggcgccg cgccgggcgc
    12301 gcaacatgcc atggattcgg tcccggtgaa tataggcgcc gcgccggctc cttcgggtct
    12361 cgactccctc gctgagcata tgcagcatct gttcgtattc gacccacgac aggttgcgat
    12421 agggataagc gccgcgaacc atgtgccaca atctttcttc gttgatgccg ccttcgggtt
    12481 gagatttgct cgcggagatg tccgctggaa ccgcgggcgt catactggcg accgtcgcga
    12541 ccatttgctg cgccagaata tcgagaggtt tttcgatcaa agcaattttg tccaactccc
    12601 cttggcgaac gctgcgaatc gcggcggcgc actgcatcag atcgtcccgg gtcaagggat
    12661 aaaaaatacc tttgggcgtt gctcccaacc agtgccctga gcggccgacc cgttggagca
    12721 gaaccgccaa tgtgcgcggc ccaccgatat ggcaaacgag atcgacatgg ccgatgtcga
    12781 ttcccagctc caacgatgcg gtcgcgacta cgaccgggta ttgagccatt ttcaattttt
    12841 gttcgacttc caagcgagtt tttcgggaga ggctgccatg atgggcgccc accttgcctt
    12901 ctcctaaacg ctcggtcaat tggtgcgcca ctcgctccac cagacgtcgc gtgtttacga
    12961 acaccaatgt cgttttgtgg tttttgattt gctcgacgat tttatcgtag acaccggccc
    13021 agatatcctg gcggataatc ggcccgagct cctcatcagg aacttcgatg cgaagctcca
    13081 gatggcgttg gtgacccgcg tcgacaatca cgcagtcggg gctgccgtcc tcgtgcactg
    13141 cacgcgaccc gaccagcagt cttgctattt cctcgatagg tttttgcgtt gcgctcaagc
    13201 cgatccgctg caaaggccgc tccgccaatt gattgagccg ctcaagtgaa agggccagat
    13261 gcgcgccacg tttatccgct gcaatggcat gaatttcgtc gatgataacg gtctcggcgt
    13321 ttttgagaaa ttcacggctg cgcggtgaac tcagcaggat gtacagcgat tccggcgtcg
    13381 tgatgaggat atgcggcggc cggcgcacca tcaattgccg ttcccgagcg ggcgtatcac
    13441 ccgaccgtac cgcggtgcga atctccggca acataattcc ttcgcggtac gcaagatgaa
    13501 agatttcatc gagcggctgc tgcaagttct tggcgatatc gttgccgaga gccttcagcg
    13561 gtgagacgta aacgacgctg gtctggtctc gtaactcgcc cgatatcgcc cgcttgaaca
    13621 gccggtcgat ggaccacaag aaagcagcca acgtcttacc ggatccggta ggagcggcaa
    13681 tgagggtcgg ttggccgcgc gcgatcgata accaaccttc ctgttgcggc agcgtcggat
    13741 tttcgaaccg ttcacggaac caacgctgga tgagcgaatg gaatgcgtcc acatgttctc
    13801 ctgtagatct caagggagaa gaacaagctt aagaggaaat tacggcaggt tcaacagatg
    13861 ctactgatag gattatttct gcggcgtgat cgattccggc tgagtcccca ttccacgctt
    13921 ctgcagctcc tccatgacga gcttggcgcc ggcgtgacct ttgttggcca attcactggt
    13981 tttgctgatc agagattcaa attgcgtgcg aagtttttgc gcttcctgca tggccgcttc
    14041 ctggtgaccg cgtgcttcgc caagattggc gcgctcgctc gcgagctgta ctgtttgaaa
    14101 accgaaccag agcaccaagg cgccgagcag aattgtcaac ggcagattcg ctcctcgttc
    14161 gacccgcggc gcggccggct cgggttcttc gatttcgttg cgctcctcgt ccatgatcaa
    14221 aacacctatt tcgggttcgc ggcgggaggt tgcgagggag tcggtgacag attgatgccg
    14281 acgcccgaca acagttcctt cagctgtcca tcattggttt tcagggccat ttcagccagc
    14341 acgcctatca cctgccggtt cagggcttcc agttgaatcg tctcggcaat gtattgttgc
    14401 cgctcgttga cgctgagctg gagtgactga ttaccggcca tcaataagat attgacgatc
    14461 accagtaaca acaccaagac cgacaatcct gttaggacca tttttcctgt tttcatgtct
    14521 cgagtcgttc ggtagctgaa tcgttcctgg cgtttgttaa gcccgctcga tttaacggat
    14581 caagctgccg agacgctccc aacgtaccca acgatcggtg ccatcccagg accagtagat
    14641 cagaaatgct ttgccgcgca cctcgtccaa gttaacgtaa ccccagaaac gactatcgta
    14701 actacgatcg cggttatcgc ccataacgaa gatgtgattc tcgggcacgg ttcgcggacc
    14761 atagtcgtca cggttcgaaa ggccggcggt ttgcggatca tcgccttcga agtgcgcgtg
    14821 ggggtcgtcg atttcttttc cgttgatcga aatcttcttg ccgcggatct gaaccacatc
    14881 tcccgccaca ccgatgaccc gtttgatgaa atccttgctg cggtcttcag gaaaaataaa
    14941 gacgatcacg tcaccccgtt tcggtttcga gaagtccacc acataatctt ccagaaacgg
    15001 cacacgaata ccgtaagcca gcttgttaac caggatgtga tcgccgatct gcaaagtggg
    15061 gatcatggag cccgatggta ttttgaacgc ctgaacaatg aacgtccgga tgaaaagcgc
    15121 cagcagtagc gccatcccga tagcttcggc gtactcgcga aaggtcgatt tgctttttgc
    15181 caatgattga ctctccgcct tggtcacttt tccggccgac ataaaactcc gatctaaact
    15241 ttcaaagcgg caagaaacgc ttcctgcggg atttctactt tgcccacctg tttcatgcgt
    15301 tttttcccct ctttttgccg ctccagcaat tttcttttgc gggtgatatc accgccgtag
    15361 cacttggccg tgacattttt tcgcagggct ttaaccgcct cccgggagat gattcggctg
    15421 ccaatggcgg cttggataac cacttcgaac atctgccgcg gaatcagctc gcgcattttt
    15481 tgcacaagat cgcgaccgcg gtaatacgcc ttatcccgat gcacaatcat cgacatggca
    15541 tccaccacgt cgccattaat gcgcacatcc agtttaacca gatccgcagc ccgatagcca
    15601 gcaagctcgt attccatgga agcatagccc cggctcaccg atttcaagcg atcgtaaaaa
    15661 tcgacgacga tttcgttcaa gggcagctca taaacgatca tcactcgctt gggcccgaga
    15721 tagcggattt ccttctgtgt gccgcgtttc tcttcacaga gcccgagcac tcctccgaga
    15781 aaatcctggg gcaaatgaat cgttgcttga atgatcggtt cttcgatctg ctcgatgtcg
    15841 ccctccggcg gcaatcgcac cggattatcg atgtgcaggg cttcgccgct cgttgtaatg
    15901 acgcggtaga ccacagtggc ggcggtggtg atgaggctca aaccgaattc tcgttctagc
    15961 cgctcccgga taatgtccat gtggagcaag ccgagaaagc cacagcggaa tccgaaaccc
    16021 agggcttgcg atgtttccgg ctcgaaggtg aaggatgaat cgtttaaccg taacttatcc
    16081 aaggcttccc gcaaaccttg gtattgattg gcgtcggtcg gataaagacc gctgaacacc
    16141 atgggcttca tcggtttgaa tcccgccagg gcttcgcctg ccgggcgatc gtccagggtg
    16201 atggtatcgc cgattcgggt ctcggctact tctttgattg ccgccatcaa aaacccgact
    16261 tctccggcgg ccaattcctc gacctcaacc ggcgccggcg caaatacccc gaggcgggtc
    16321 acttcaaaag cacggccgct aaacatcatg cgaatgcggg agcctttggc gactttgcca
    16381 tcgaacacgc gcatcataac gacggcgcca tgataaggat cgaaccaaga atcgataatc
    16441 aacgcgcgca aaggatcatc cacggttcct ttgggatgcg gaaaacgctg cacgatacct
    16501 tcgagaattt cttcggtgcc caccccttcc ttggcgctgg ccaggatggc attggaggca
    16561 tccaggccga tgacatcctc aatgtctttg cgcactcgtt cgggatcggc gctaggaaga
    16621 tcgatcttgt ttatgaccgg aaggatttca agatcgtggt ccagcgccat gtgaacgttg
    16681 gccacggttt gagcctcaac gccctgcgcg gcatcgacga tcagtaatgc tccctcacag
    16741 gctgccaagc tgcgcgacac ctcgtaagtg aaatcgacgt ggcccggagt gtcgatgagg
    16801 ttaagcttgt aatccttgcc attcttggcg cggtaacgca ggcaaacggg gctggctttg
    16861 atcgtgatgc cgcgctcccg ctccaggtcc atgctgtcca gtatctgatc ggttttctcc
    16921 cgggcgctca ccgcgccggt aaactcgagc aggcgatcgg ccaaagtcga tttgccgtga
    16981 tcgatatgcg cgatgattga aaaatttcgg atatgctcaa ggctcatgaa atctcgttct
    17041 ggagataata atctatcgtc tgcgtcagcc cttcctcaag ttcgcgctcg ggccgccaat
    17101 tcaggacgcg gagggccaag ccattttcga gagagctgcg catctgctcg ccggttttcg
    17161 ccggcccgtg gactgcttcg accttgctgc gggaccgctc ctgtaacatc cggcaaagag
    17221 tcacaacgtc ggtttcgcgg ccggtgccga tgttgatgcc gcccacatgg cccgtttcca
    17281 gagcggcgat attggccctg acgacgtcgc cgacaaaaac ataatcccgc gtctgctttc
    17341 cgtcgccatt gatggtcgga ggctttccgg caagcagctg tgaaataaag atggcgacaa
    17401 cgccagcctc gcccttggtt gattggcgcg ggccgtagac attggcataa cgcaaggcca
    17461 cgtaacgcat cccgaacgcc atataataat atgcaagata taactcgccg gtcatcttac
    17521 tgacaccgta agggctcatc ggcttttttg gatgatcttc atcagctggg atcggctcct
    17581 catcgccgta aatggcgccg ccggatgaag caaaaacaac ttgagcagta ccggcttttt
    17641 tgcatgcctc gagcaaattg aggaagccca caatattgac gcgcgcatcg aacagaggat
    17701 cttccaccga atgacgcaca tccatctgcg cagcatgatg attgagcacg tcaggtttga
    17761 tcttttgaat cattctggcg gcttccgcat cggcgacgtt gaatttatga agcagcgcgt
    17821 tggggtttag attgagcttg cgtcccgtgg agaaatcatc gatgacatgg acttcatgac
    17881 cttggtgcag gtaggcatcg actatatgcg atgcgataaa acccgcgcca ccggtcacaa
    17941 caatcttcat ggctgtcctt tcagtcgcgc gcgaaagtag tcgatagttt ttttcaaccc
    18001 ttcgtccaag tttaccttcg gctcccagtt caacagtttt ttagctttgg taatatcagg
    18061 ctggcgcacc tgcggatcat cttgcggcaa cggccggtaa gagatctcgc ttctggaccc
    18121 ggtgagttgg agaattttct tggcaaattg caaaaccgtc atctcatggg gattgccgat
    18181 attcaccgga tagtgctcgc aagactcgag caagcggcca atccccgcga ccagatcatc
    18241 gacgtattga aagctccgag tttgctcgcc ctgaccgtaa accgtaagcg gctcaccttt
    18301 gagagcctgg agaataaagt tcggcacaac ccggccgtcc tgcatgcgca tgcgcggtcc
    18361 gtaggtgttg aagattcgca caatgcgcgt gtccaggccg tggtaacggt gatacgccat
    18421 ggtcattgct tcggcaaagc gtttcgcctc atcgtacacc ccgcgagggc ccaccggatt
    18481 gacgttcccc cagtattctt catgctgcgg acggaccagg ggatcaccgt agacttcaga
    18541 tgtcgaagcc agcaaaaaca ccgcattttt tgccttggct aatcccaagg cattatgagt
    18601 gccgagtgag ccaactttca gagtgggtat cggcagttcc aaataatcca ccggacttgc
    18661 cggtgatgca aaatgaagaa cgaaatccag gggctgctga acttcgatat aacggctgac
    18721 gttatgatcc atgaaggtga accgacggtt ggcggcgaag ctggcgatat tctgctgcgc
    18781 tcccgtcgaa taattgtcca tgcagatcac gtcgtgtcct tgacttagaa agttctcaca
    18841 aagatgagag ccgataaatc cggcgccgcc ggtgatcagg tatttagcca tttgtgcgtc
    18901 ctatcgagta gtagtcgaag ccgagctttt tcagctctcc gggatcgtag atattacgtc
    18961 cgtcaaagat gatcggttct ttgagcagtt ccttgatgcg ttcgaaatcc ggccgacgaa
    19021 attcgttcca ttcggtaagc accagcagag cgctggcgcc gtccagcgca tcgtaatttc
    19081 gcgccgcata aacgacacgg tcgcggaata tttttctggc ttcttcggtt gcctcaggat
    19141 cgaacgcctg gatccgggcg cccgcttgca gcagggcttc gatcacaacg atggccggcg
    19201 cgtcgcgcat atcgtcggtg cgcggtttaa acgcaagccc ccagatcgca aacgtctttc
    19261 ccgacagctt tgcgccgaaa cggcgcttga ccttttccac cagcactctc ttctgccgct
    19321 cgttgacttg ttccacagcc ttcatcagag acatttcttc ttgatcaccg cccatgctga
    19381 taatggctcg gatatctttg ggaaagcagg agccgccata gcctactccg gggaagatga
    19441 aatgctgtcc gatacgccgg tccaatgcga tggcgcggcg cacgtcattg atgtccgccc
    19501 ccatatactc gcataaacgc gagacttcgt tgataaacga tatttttgtc gcgagcatgg
    19561 cgttggccgc atacttgctc atctcggcgc tgcggctatc catcacgagt atctgattgc
    19621 cggtgcgcag aaacggttca taaagctctt tgacggtttc caaagcctcg gggtcatcgc
    19681 cgcccagaac tacccggtcg ggtttcatga agtcttctac ggctgc
//
LOCUS       DASUVS010000193         7161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_299042, whole genome shotgun sequence.
ACCESSION   DASUVS010000193 DASUVS010000000
VERSION     DASUVS010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299042"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="VF182_10785"
     CDS             <1..1365
                     /locus_tag="VF182_10785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6437606.1"
                     /translation="GTATRIVGISMPTIASSLGTDLLGVSWALLSYQLSNIGLSIVFG
                     RLSDLWGREKVFALGFLVFSAGSLLCGLSQSLIQLILFRFLQGIGAAMLQSSSRALAA
                     ESVPENLAGRAQGYMTTAHHVGFIIGPSIGGLMIDYLSWRWSFFFLVPIGICGMLLSW
                     LNVKRRPQTFPSRSASIDYLGAALLFATTSLLVLLLDRRTQQMVAPSSKIALVILFSV
                     CLGGLIFHEARTRNPILNLKLFKIRRFSMSVISLLVVASCYALTAFLLPFYLQDVLGL
                     SATSVGMLFMAPSVITVALAPASGYLADRLGPRLPASVGVAFMIVSLGMGAFLRVDSH
                     WLLPALLVIVSAITNGIFNPANSMAMIALMPAEHRGFASAVNHVTFGFGNVLGVALSS
                     LLMSVAFEHHTGIAGTALTTANPSGFVAALNTTFIIAAAWSTVAVAASLIGSARKSSH
                     PGQR"
     gene            complement(1379..2362)
                     /locus_tag="VF182_10790"
     CDS             complement(1379..2362)
                     /locus_tag="VF182_10790"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6437607.1"
                     /translation="MFTARRATLPASAKVILLKEDFTIQRRQKLAPPKPLSGKERRLL
                     NPNHGLVKLFQRNMDRLISRFLYPHLSAIWHPYAWLIEHRFRVAETTLSPAGWPQGLP
                     PLRVLLLSDIHCGIFLKPESLAEIVKFLMQLEPDLVLVTGDIVTGHSRELRPFIDALL
                     PLSRAPRGAWFCFGNHDYFGGDPEEIRAGLQAIGIITLKNESVKISHGPGSFVLGGID
                     DLILGKPDWARMASKHGAPHLLLSHNPDYFYEAADQGVALVVSGHTHGGQIRLPNGPP
                     IVRQSRFCLDEGAYSFQSSLLVVTRGLGSILLPWRWGADPEAVMIEIVAAK"
     gene            2594..4204
                     /locus_tag="VF182_10795"
     CDS             2594..4204
                     /locus_tag="VF182_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437608.1"
                     /translation="MTKTKRLIVFLIALSLCGWPALVQAAEGPAAQQPRSGGELVFAV
                     GELPPSFDGHRETTFGMLHPVAPHYSTLLRFDPQNYPKIVGDVAERWSFSRDGLTATF
                     TLRKGIKFHDGTPLTARDVKASYDKIIFPPEGVASARKASFAMVDKIEAPDDATVIFR
                     LKHIAASFLPNLASPWNFIYSAERLKKDPRWYEKNILGSGPFIFGEHVAGSHWTGKRN
                     PNYFMPGRPYLDSFRAVFIRDTAPRVAAVRSGQVLAEFRGFNPAARDDIVKALGNKAT
                     VQESPWACNILVALNNEKKPFNDVRVRRALNLAIDRNEASKALSQISVMKYVGGVFRP
                     GSEFATPPAELTKLAGFGKDIETSRKEARRLLKEAAVPEGFSFVLKNRNVKEPYEVSG
                     VFLVDQWRKIGLNVTHTPQEGGPYFSDLRQGNFDAGVDFACDFMDEPDLYSLKYVSTE
                     KSPINYSRYKDATLDELYERQSRMLDLKDRLPLLRQMERRAIADQAYQFPLLWWQRIV
                     PHSSRLKGWKIGPSHYVNQDLRDVWLAP"
     gene            4241..5191
                     /locus_tag="VF182_10800"
     CDS             4241..5191
                     /locus_tag="VF182_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6437609.1"
                     /translation="MGQYILKRVLLMIPTLFGVAVLVFVLLRIAPGDIVELKYAGSGT
                     FAPKEAIERERVQLGLDKPVWQQFLDWSWGIVRMDFGVSMWTGRPIAEEIRIRLELSL
                     QLAIMATLVAILIALPCGTLAALKQDTWVDYLVRIFSIGGLAVPSFWFGIVIILLFLI
                     FFKWLPPLTFTSFWTDPLANMSQMIWPALAVGYRYSAVATRMTRSAVLEVLGEDYVRT
                     ARAKGLRENAVVLKHALRNALLPIVTVIGLEFAFLIGGLVVTEQVFNLNGIGMLLVES
                     ITHRDYTMTQALVLLVSFAFIAMNFFLDLLYVWLDPRIQY"
     gene            5226..6089
                     /locus_tag="VF182_10805"
     CDS             5226..6089
                     /locus_tag="VF182_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020021157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6437610.1"
                     /translation="MTLFRAQRPWQAVLRFVQRRPLGAVGAAIVILMALAALLAEIIA
                     PFDPLQNDYGAMFHAPGATHWLGTDSFGRDVLSRLLYGSRTALWVGFSSSLFASTVGA
                     ALGVTAAYFGGKVDLLLQRLLDILISFPLIVLALVVVALLGSGIWNVIVAIAIPMIPR
                     AALVLRSSSLALRQMLFIEAALTLGAGHARVIFSHMLPNVVAPFLIILTAFLGQAILL
                     EASLSFLGLGVAEPTAAWGLMLRGAAVDFAERAPWMALFPGLAISLAVFAFNVFGDSL
                     RDALDPKLRVP"
     gene            complement(6122..6955)
                     /locus_tag="VF182_10810"
     CDS             complement(6122..6955)
                     /locus_tag="VF182_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6437611.1"
                     /translation="MSGRFLDLQQGKVWLLENGRGAPLLYLHGFVDVHSIKESWMPFH
                     DLLATGSRVIAPAHPGCSKSDENQDIEAIEDVVFHYLEFLDGLNIPQFDLIGSCVGGW
                     IAAELAARHPEKIRKMVLIGATGLFVQSALIGDVFMMAQPERGSSYAGLRQMLFSDHD
                     HSKALELFPDGMGAIEDELRRYQMLRFGSRIGFKPPYFYNRPLANRLHRITAPALVIW
                     GEHDRMVPLSHGETYAQSMPQAQPLKVIPGAGHSVHVEQPELAARIISDFLAVDTASS
                     H"
     gene            complement(6952..>7161)
                     /locus_tag="VF182_10815"
     CDS             complement(6952..>7161)
                     /locus_tag="VF182_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437612.1"
                     /translation="QRIWNLIERAKVGNLLIQFHIGNMSKELALKSMTLFAEQVAPRL
                     RESSMSFFEQEFPGVDIEQQKGATL"
BASE COUNT         1439 a         1930 c         2053 g         1739 t
ORIGIN      
        1 ggcactgcga cgagaattgt cggcatatcg atgccgacaa ttgccagcag cttgggaacc
       61 gatctgctcg gcgtttcttg ggcgctgctc tcctatcagt tatcgaacat cggcctatcg
      121 atcgtttttg gccgtctgtc agatctctgg gggcgggaaa aagtttttgc cttaggattt
      181 ttggttttta gcgccggctc acttttgtgc ggcctgtccc agtcgcttat tcaactgata
      241 cttttccgtt ttttgcaggg catcggcgct gcgatgttgc agtcctccag tcgggccttg
      301 gctgcggaat cggttccgga gaacctcgcg ggaagggcac agggctatat gacaacagcg
      361 catcatgtcg ggtttattat cggcccaagc atcggcggcc tgatgatcga ttacctgagc
      421 tggcgctgga gtttcttttt tctggtgccg atcggcatct gcggcatgct gctgagttgg
      481 ttgaatgtaa agcggcgtcc ccagaccttc ccatctcgct ctgcttccat cgattatctc
      541 ggcgccgctt tgctattcgc gacaaccagt ctgttggtgc tgctgctcga caggcggaca
      601 cagcaaatgg ttgcaccgag ctcgaaaatt gctttggtga ttttatttag tgtctgcctt
      661 ggcgggttga tttttcatga agccagaacc aggaatccga ttctcaacct gaaattgttc
      721 aaaatccgca ggttcagcat gagcgtaatc agcttgctgg tcgtcgccag ttgttatgcc
      781 ctgaccgcat ttctcctgcc gttctacctc caagacgtcc tcggcttaag cgcgacgagc
      841 gtaggcatgc tattcatggc gccgtcggtg ataaccgtag cgttggcccc agcaagcggt
      901 tatctcgccg accgtctcgg gccgcgcctg ccggcgagtg tcggcgtcgc cttcatgatt
      961 gtttcgctgg gtatgggtgc gtttttgcgt gtcgattcac attggctact gcccgcgctc
     1021 ctggttattg tcagtgccat caccaacgga atcttcaatc cggccaattc catggcgatg
     1081 atcgcgctga tgccggccga gcaccgcggt ttcgcctcgg cggtgaatca cgtcacgttt
     1141 ggtttcggca acgttctggg tgtcgccttg agcagtttgc taatgtcagt agccttcgaa
     1201 catcataccg gcatcgccgg aaccgctctc acaacggcaa atccgtcggg gtttgtagcg
     1261 gctctgaata ccacatttat cattgctgct gcctggagca cggttgcggt cgcggcttct
     1321 cttatcggca gcgcgcgaaa atcttcgcat ccgggacagc gttgatggtc acgcgagact
     1381 attttgctgc aacgatctcg atcatgaccg cctctgggtc ggcgccccag cgccacggca
     1441 ataggatgct gccgagaccg cgggtgacca ccagcagcga agattgaaaa gaataagctc
     1501 cttcgtcaag gcagaaccgg ctttgccgga cgatgggcgg cccattaggc aggcgaatct
     1561 gaccgccatg cgtgtgcccg gaaacgacaa gcgccacccc ttgatctgcg gcctcataaa
     1621 aatagtcggg gttatgcgac aatagtagat gcggtgcgcc gtgtttagag gccatgcgcg
     1681 cccaatcggg cttgccaagg atgagatcat cgatgccgcc aagcacaaag cttcccgggc
     1741 catgggatat ttttacagac tcgttcttga gcgtaatgat accgatggcc tgcaaccctg
     1801 cgcgaatctc ttcaggatcg ccgccaaaat agtcgtgatt gccgaagcag aaccaggcgc
     1861 cgcgtggagc gcgcgacaag ggcaatagag catcgataaa tggccgcagt tcacgcgaat
     1921 ggccggtgac gatatcccct gtcaccaata ccagatcggg ttccagttgc atgaggaact
     1981 tgacgatttc ggccaagctc tcaggcttaa gaaaaatgcc gcagtgaatg tccgatagca
     2041 atagcacccg aaggggtgga agcccctgcg gccagccagc gggcgataga gtggtctccg
     2101 ctactcggaa acgatgctcg atcagccagg catacgggtg ccaaatggcc gagagatgag
     2161 ggtacagaaa gcgtgatatg agccggtcca tattgcgctg gaaaagcttg accagcccat
     2221 gattcggatt gagcaggcgc cgttccttgc cgctgagcgg cttcggtggc gcaagttttt
     2281 gtcgtctctg tatggtgaaa tcttccttca ggaggatgac cttggctgac gctggtagcg
     2341 ttgcccggcg cgccgtaaac aagaactatt ctgtcttaaa ttcttccccg ggacaatgat
     2401 ccaacagcgc ttcgggctgt attggcgagc taaaccatta gaagaacatt tgaagagttc
     2461 cgattgcagc gcgaccaaac tgcctaacag cttgacatca cgaagacgaa tcacctattt
     2521 tcgcgcagtt gtatgcttat actccgaaac gtgaagagat gatcgattgt ccagccacag
     2581 atgagagggc tctatgacca aaaccaagcg actcatcgtg ttcctgattg cactaagttt
     2641 atgcggatgg ccggcgttag ttcaagcggc ggagggtccc gcagcgcagc agccgcgttc
     2701 cggaggggag cttgtctttg ccgtcggcga acttccgccg tcgttcgacg gacaccgcga
     2761 aacaacattc ggcatgctgc atccggtagc cccgcattac agcacgctgc tgcgcttcga
     2821 tccgcagaac tatccgaaaa tcgtcggcga tgttgccgag cgctggtcgt tctcgcgcga
     2881 tggcttgaca gccacgttca cgcttcgcaa aggcattaaa tttcacgatg ggactcccct
     2941 gacggcgcgc gatgtcaaag ccagctatga caagatcatt ttccctcccg aaggggttgc
     3001 cagcgcgcgc aaagcttcct ttgccatggt cgataagatc gaagcgccgg atgacgcaac
     3061 ggtgattttt cgtctcaagc acattgcggc gtcctttttg ccaaatttag cgtcaccttg
     3121 gaatttcatt tatagcgcag agcgactgaa gaaagaccct cgttggtacg agaaaaatat
     3181 tctgggatcg gggccattta tcttcggcga gcacgtcgca ggctctcatt ggaccggtaa
     3241 acgaaatccg aattatttta tgcccggccg gccctaccta gatagttttc gagccgtctt
     3301 catccgcgac accgctccgc gtgtggctgc cgtaagaagc ggtcaagttc tcgccgaatt
     3361 tcgtgggttc aatcccgccg cccgagatga cattgtcaaa gcgctgggga acaaggcgac
     3421 cgtacaggag agcccctggg cgtgcaatat tttagttgcg cttaataacg agaaaaagcc
     3481 gttcaatgat gtgcgcgtca ggcgggcgtt gaatctcgcc atcgatcgca acgaggcgtc
     3541 caaggcgttg tcacaaatct cggtgatgaa gtatgtaggc ggcgtctttc ggccgggctc
     3601 ggaattcgcg acaccgccgg ccgaactgac gaagctcgct ggatttggca aggacatcga
     3661 aacgtcgcgc aaagaggcgc gtcggttgct gaaggaagcg gcggtgccag aggggttctc
     3721 atttgtgttg aagaaccgca atgtcaagga gccatatgaa gtgagcggtg tgtttctcgt
     3781 cgatcaatgg cgcaaaatcg gtcttaacgt gacccacacg ccgcaggaag gcggccccta
     3841 ttttagcgat ctgcgacaag gaaacttcga tgccggcgtg gatttcgcct gtgatttcat
     3901 ggacgaaccg gatctttatt cgctgaagta tgtgagcacc gaaaagagcc cgatcaatta
     3961 ctcgcgctat aaagatgcta cgctcgacga gctttatgaa cggcagagcc gcatgctgga
     4021 cctgaaggat cgattgccgt tgctacgcca gatggaaaga cgcgcaatcg ccgaccaggc
     4081 ctatcagttt ccgcttctat ggtggcagcg gattgtgccg catagcagcc ggctcaaagg
     4141 ctggaagatc ggtccaagcc attatgtcaa tcaggatttg cgcgacgtct ggttggcgcc
     4201 gtaagcgcct gacgattgac atcgccgcgc tcggatctca atgggacagt acatactcaa
     4261 gcgtgtgcta ctaatgattc cgacgctctt cggggttgcc gtgttggtct ttgtcttact
     4321 ccgaatcgcg ccgggagaca tcgttgagct caagtacgcc ggcagcggca cgtttgcgcc
     4381 caaggaagca atcgagcgcg aacgggtcca gctcggactt gataaaccgg tatggcagca
     4441 attcctggat tggtcgtggg gcattgtgcg catggacttt ggcgtttcca tgtggactgg
     4501 tcggccgatt gcggaagaaa tcagaatacg acttgagctc agtctccagc tggcgatcat
     4561 ggctactctg gtggctattc tgattgcgct tccctgcggc accttggctg cgttgaagca
     4621 ggacacctgg gtggactacc tggtgcgaat tttttccatc ggcggccttg ccgtgccctc
     4681 gttctggttc ggcattgtta tcattttgct gtttttgatc ttctttaaat ggcttccgcc
     4741 gctgactttt acttctttct ggaccgatcc gctcgctaat atgtcgcaaa tgatatggcc
     4801 tgccttagcc gtgggatacc gctattcggc cgtggcaacg cgcatgacgc gatcggcggt
     4861 gttggaagtt ttgggtgaag attatgttcg cacggcccgc gccaaaggcc tcagggaaaa
     4921 tgcggttgtc ttgaaacatg cgttgcgaaa cgcgctgctg ccgatagtta ccgtgatcgg
     4981 tttggaattt gcttttctga tcggcgggct ggttgtcacg gagcaagtct tcaatctcaa
     5041 cggcatcggc atgctgctcg tcgaatcgat cacgcatcga gactacacga tgacccaagc
     5101 tttggtgctg ttggtgtcgt ttgcgtttat cgcgatgaat tttttcctcg acttgctcta
     5161 tgtctggctc gacccgcgca tccaatacta aggaagtatc acagcgtcag atagagacgg
     5221 gtaggatgac tttattccga gcacaacgac catggcaggc ggtccttcgt tttgtccaga
     5281 ggcggcctct cggagccgtc ggcgcggcta ttgtcatttt aatggcttta gcggccttgc
     5341 tggcggagat catcgctccg ttcgatcctt tgcagaacga ttatggggcg atgtttcatg
     5401 ctccgggcgc aactcactgg ctgggaaccg attccttcgg gcgcgacgtg ctctcccggc
     5461 tcctctatgg ctcccgcaca gccttatggg ttggattctc ttcttcgctt tttgccagca
     5521 ccgtaggcgc tgctctcggt gtgacagccg cctactttgg cggcaaagtt gatcttcttc
     5581 tgcaacggct gctcgatatc ctgatatcgt ttccgctgat tgttctagct ctggtggttg
     5641 tggcgctgct tggctcaggg atttggaatg tgatcgtggc gattgcgatt ccgatgattc
     5701 cccgtgccgc tctggtgctg cggagcagct cactggcatt gcgccagatg ctttttatcg
     5761 aggcagcgct gacgctgggc gcgggtcacg cccgtgtgat attcagtcac atgctgccga
     5821 atgttgtcgc gccttttctg atcattctga ccgcttttct cggccaggcg atccttctcg
     5881 aagcttcgct ttctttcctc ggtctgggtg tggccgaacc gacggcggct tggggattaa
     5941 tgcttcgtgg agctgcggtg gatttcgccg agcgcgctcc gtggatggcc ttgtttccgg
     6001 gtctagcgat cagcctggcg gtgttcgctt tcaatgtctt cggagactcg ttgcgtgatg
     6061 ctctcgatcc aaagttgcgg gtgccttgag ttggacaacc tcgcataggc gcgagaatcc
     6121 gctagtgact gcttgcagtg tcaactgcta aaaaatccga aatgattcgt gccgcgagct
     6181 cgggctgttc gacatgcacg ctgtgccctg caccgggaat gactttgagc ggttgcgctt
     6241 gcggcatcga ctgggcgtaa gtctcgccat gtgataacgg caccatgcgg tcgtgttccc
     6301 cccagatcac cagggcaggc gcagtgatgc gatgaagtcg gttggccagc ggtcgattat
     6361 agaagtaggg tggtttgaag ccgatacgcg agccgaagcg cagcatctga tagcgtcgca
     6421 gctcgtcttc gatcgcaccc atcccatcgg gaaaaagctc cagtgctttt gagtgatcgt
     6481 gatccgaaaa aagcatctgg cgcagcccgg cataactaga gccgcgctcg ggctgagcca
     6541 tcatgaagac atctccgatc aaggcactct gcacgaacag tcctgtggcc ccgatcaaaa
     6601 ccatcttgcg aattttttcc gggtggcgcg ccgcaagctc cgcggcaatc cagccgccga
     6661 cgcagctgcc gataaggtcg aactgcggaa tgttcaatcc gtccaggaac tcgagatagt
     6721 ggaagacgac atcttcgatc gcttcgatgt cctggttctc gtcgcttttc gagcagccag
     6781 gatgcgccgg cgcaatcact cgagaccctg tggcgagaag gtcgtgaaaa ggcatccagc
     6841 tttccttgat ggagtggaca tcgacgaagc cgtgcaggta aagcagagga gcgccacggc
     6901 cgttttccag gagccaaacc ttgccttgtt ggagatcgag aaagcggccg ctcacaaggt
     6961 cgctcccttt tgctgctcaa tatcaacgcc cggaaattct tgctcgaaga agctcattga
     7021 gctctctcgc aggcgcggcg cgacttgttc cgcgaacaaa gtcatgctct ttagcgcgag
     7081 ctccttggac atgttgccga tgtgaaactg gatcaagagg tttccgacct tggctcgttc
     7141 gatgagattc cagatccgct g
//
LOCUS       DASUVS010000194         5502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_79909, whole genome shotgun sequence.
ACCESSION   DASUVS010000194 DASUVS010000000
VERSION     DASUVS010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79909"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="VF182_10820"
     CDS             <1..1093
                     /locus_tag="VF182_10820"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6437613.1"
                     /translation="EVLMKAIVRSTGVLLLCTVLFGATTVQAWGAAAKVNFDEKAVAE
                     FYKGKTVRIIVGFSAGGGYDAYSRVIGRHLHKHIPGNPTVVVDNMAGAGSILAANHIS
                     NVAPKDGTIIGNFSGQIILEQLFANSAVQYDMTKFPILGVPIGETYLLVVTKKPGVTK
                     YEEVLGPKGKQIIMGGIPGSTVEQAPIIMRDALGANIKVVSGYKGTSDVRLAIDSGEV
                     DGLFNSWTSLKITSLDKIKSGEWLVLAQIGEEPIRDLPNSSNIPTIPQVAKTAEQREL
                     LRFGIAAPNQFGKIYVLPPGAPQDRASALEAAFTKTFEDKEFLAEADKGRLEIDPVGA
                     EKAQGLIRDLMGMSPAVKTQLQKMLRTPK"
     gene            1293..2318
                     /locus_tag="VF182_10825"
     CDS             1293..2318
                     /locus_tag="VF182_10825"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6437614.1"
                     /translation="MLRKLSWLFVLFVLEVASPRGVFAQEPFYTGKIVRIIVGFSAGG
                     GFDTYARVIARHMGKHIPGGPAIIVENMPGAGSLIAANHGYNTAKPDGLTINNWIGGL
                     VLQAVLGGKGVQFQAPNFGWVGALQDSQSCGLTKASGVSNVQEWLAAKNPIKLGGTAS
                     GSTDVDIPKILKATLALPIQLVEGYKGTADIRIAADSGEVSGYCGTWAGMKSTWSQAL
                     ESGNAHIVLQINSKKHPDLINVPNAVDLAKTDEARELIKMGIHYQADISRNFSLPPGT
                     PKERVAILQGAFLQTTKDKEFLADAQKAKLSVEAISGDDVSRIVSDYSAMKPEVIKKL
                     KEIMLPN"
     gene            2364..3170
                     /locus_tag="VF182_10830"
     CDS             2364..3170
                     /locus_tag="VF182_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437615.1"
                     /translation="MDFSDEVKKTLTAILAKYAAGNAEIVRTYFSRPRSREQDIAWLK
                     CQAARELTTAWKFLDNLKELQPKFEKELKRKDYESLARAFSEELEHYRLFVTILEEEF
                     GERIDVDELLTLGVWSDNERFPENTKKARYEKELRASGDEMAKIALGIGEGGGSGWLI
                     GLSRISGGKLEQRMAHAAKEISHDEMFHGPIHIAAAGRRIKRPEQLETAKTVLARYLQ
                     YHLRMKNEQFGNPLSKERLEEIDQGKVQPLEGVDFSPIGREREDLAAYAL"
     gene            3493..4518
                     /locus_tag="VF182_10835"
     CDS             3493..4518
                     /locus_tag="VF182_10835"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6437616.1"
                     /translation="MAKYLASLVTLILAITLNLSTSAFSQESFYKDKVIRIIVATAAG
                     GGYDLYTRTMARHLRKYIPGEPTIVVENMPGAGHLIGANYVYKVAKPDGLTIGHFIGS
                     LFLQQLLVKPGVEFEALKFNFIGVPAQDNYMIGLSKTTGITSMEQWLASKRILKIGGI
                     GAGSATDDIPKVLAATLGLPLQVVAGYKGTSEVRLAFNSGEIEGICNAWESFKSTWRK
                     ELDANEVALVLQAIPTRHPDVPNVPLAIDYAKTDEAKRMIRSVIHTIAPTVRPYVLPP
                     GTPKDRVEMLRRAFMSTMKDQEFLADAAKAKLDINPLDGATLESNVREVFNLDPALIP
                     RLKEILK"
     gene            4557..>5502
                     /locus_tag="VF182_10840"
     CDS             4557..>5502
                     /locus_tag="VF182_10840"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX6437617.1"
                     /translation="MTESTRVPQGLKSVSELILRSYWHPVATSSELRNAPLAASLLDE
                     PIVVWRTKDKVAAFRDLCIHRGTALSLGWIQDDNLVCPYHGWTYAPDGNCVRIPSLPA
                     GHAIPAKARATAYCNVRERYGLIWVCLAEPKAPIPEIPELEDPNYHTFFHSTDMWETG
                     AARMIENFIDTSHFPYVHPGINATPDDPVIPEFEVKQNGFELSFETKFTAPPSGAFRG
                     PSALASYSAFTEGRRQYRVVLPFTAQAIRPMEGARRQLVSIIAAPISAKRMRYYTFSS
                     RNFALDQPDDAFRELIQVIFAQDRKIVESQRPEELPLDL"
BASE COUNT         1374 a         1370 c         1517 g         1241 t
ORIGIN      
        1 tgaggtgctt atgaaagcga ttgtgcgatc caccggagtg ttactccttt gcacggtgct
       61 gtttggagct acgacggtcc aagcctgggg agcggctgcc aaagtcaact tcgacgagaa
      121 agcggttgcg gagttttaca agggtaagac ggtacgcatc attgtcggat tttcggccgg
      181 tggcggttac gatgcttatt cgcgtgtgat cggccggcat ttgcacaagc atattcccgg
      241 taacccgact gtggtcgtcg acaatatggc cggcgccgga agcattctgg cggcgaacca
      301 tatcagcaac gttgctccta aagacggcac gatcatcggc aatttcagcg gccagattat
      361 tttggagcag ctgttcgcca attccgccgt tcagtacgac atgacaaaat ttccgattct
      421 cggagtgcca atcggagaaa cttacttgct ggtagtcacg aagaaacccg gcgtgacaaa
      481 gtatgaagag gtgctcggcc ccaagggaaa acaaatcatc atgggaggta tccccggatc
      541 taccgtggag caggctccca tcatcatgcg cgacgcgctg ggcgccaaca tcaaagtggt
      601 gagcggctac aaaggcactt ccgacgtccg gctcgccatc gatagcgggg aagtggatgg
      661 tctctttaac agctggacct cgctcaagat tacctctctg gacaagataa aaagcggcga
      721 atggctcgtg ctcgcgcaaa tcggtgaaga gccaatccgc gatctgccga acagcagcaa
      781 tattccgaca ataccgcagg tggccaagac cgccgagcag cgggaattgt tgcgcttcgg
      841 catcgcggcg cccaatcagt tcggaaagat ttatgtgctg cctccgggag caccccaaga
      901 tcgtgcttcg gcgctggagg cggcgttcac caaaactttc gaggacaaag agtttctcgc
      961 cgaagccgac aagggccggc ttgagatcga cccggtcggc gcggagaagg cgcagggact
     1021 aatcagggat ttgatgggca tgtcgccggc ggtgaaaacg cagctccaga agatgctcag
     1081 gacgcctaaa tgaggcgtaa gaatacgaat cgttggcaga tatccaatgg tgtccatcca
     1141 ggttgcagcg tcgatgccca gtgcttagcc gcatggacat tttctgacct caatgaagcc
     1201 ttcgacttgc actcagtgaa tcgatccgca acgggtcctc tactgatccg gtgatcagat
     1261 ccagtaaatc tttgctgcgg aaaggaactc taatgttgag gaagctgagt tggttgtttg
     1321 tgctatttgt gctcgaagta gcatccccga ggggagtctt cgcgcaagaa cctttttaca
     1381 caggtaaaat tgtgcgcatc attgtaggtt tctcagcagg cggaggattt gacacgtatg
     1441 cacgagtgat cgcgaggcat atgggaaaac atatcccggg cgggccggca atcatagtgg
     1501 agaacatgcc tggtgcggga agtttgattg cggctaacca tggttacaac accgccaagc
     1561 cggacggact gaccattaac aactggattg gcgggttggt gctccaggca gtcttgggtg
     1621 gcaaaggtgt ccaatttcaa gcgccgaatt tcgggtgggt tggagcgctg caagatagtc
     1681 agagctgcgg gctcaccaaa gcaagcggtg tatcaaacgt tcaagaatgg ctggcggcaa
     1741 aaaatccgat caagctcggg ggaacagcat cgggaagtac ggacgtcgac ataccgaaga
     1801 tcttaaaagc taccctggcg cttccgatcc aacttgtcga gggatataaa ggaactgctg
     1861 atatccggat cgcagcggat tccggggagg tttccggtta ctgcggtacc tgggccggga
     1921 tgaaatccac ttggagccaa gcgttagagt caggcaatgc tcacatcgtg ctccagatca
     1981 attcgaagaa acaccccgat ctcatcaacg tgcccaacgc tgtggatttg gcaaagaccg
     2041 atgaggcacg cgagcttata aaaatgggaa tccattacca agccgatatt tctcgaaatt
     2101 tctcgctgcc gccgggcact ccgaaagagc gcgtagcgat ccttcaaggc gcttttttac
     2161 aaaccacgaa ggacaaagag tttcttgcgg atgcacaaaa ggcgaagctt tccgtggagg
     2221 ccatttcggg cgacgatgtt tctcgaattg tgtccgatta ttccgccatg aagcccgaag
     2281 taataaaaaa attaaaagag atcatgctgc caaactagtt catccagacg cgagacggat
     2341 tgcccataga aagggggaac gaaatggatt tttccgatga agtgaaaaaa acactgacag
     2401 caattcttgc caaatatgcc gcgggcaacg ccgaaattgt ccgaacctat ttcagccgcc
     2461 caagatccag agagcaagat atcgcctggt tgaagtgtca ggcggcacgc gagctcacga
     2521 cggcttggaa gtttttggat aatttaaaag agcttcagcc taagtttgaa aaggagctca
     2581 agcgcaagga ctatgagagt ctcgcgcggg cgttttccga agaattggaa cactatcgtc
     2641 tgtttgtgac gatcctcgag gaagagttcg gggaaaggat cgatgtagat gagttactga
     2701 cactcggcgt ttggagtgat aacgagcgct tcccggaaaa tacaaaaaaa gctcgctatg
     2761 agaaggaact gcgcgcttca ggggatgaga tggctaagat cgccctcggt atcggcgagg
     2821 gtggtgggtc cggatggctt atcggattga gccggattag cggaggcaag ctggaacagc
     2881 ggatggcgca tgccgcaaaa gagatctccc acgatgaaat gtttcacggg ccgattcaca
     2941 tcgccgctgc cggccgccgc atcaaaaggc cagagcaact cgaaaccgcg aaaaccgtct
     3001 tagcccgcta cctgcagtat catttgcgga tgaaaaacga acagtttgga aatccgcttt
     3061 caaaggagcg tctcgaagaa atcgatcaag gcaaggtcca gcctttggag ggagttgatt
     3121 tctcgccgat tggacgagag cgcgaagatc ttgcggcata cgcattgtaa cttcgctttc
     3181 tagcgtattg tacccgagat cttatctcat tcattgaatg gatggatgcg gcgacaaccg
     3241 ctcttttact ctaagcaatc aacagaaatt atccggaaga ccggagagca cttgccgcgg
     3301 aatctctcag gggcgacgac cgcagccgac cttacgaagt tcctcccaca agtcggacat
     3361 tacgatgtcg acctgctcaa cttgactgct gatagggtgt taacggtcac gaatcttggc
     3421 tagctcctta tgactgaccg gcgatcatga atcccatcga acaatccaag gcaaattgcg
     3481 cgaggaggga gtatggctaa atacttggcg agtctcgtaa cactaattct tgctatcact
     3541 ctaaaccttt cgacctccgc gttttcacaa gaatcgttct ataaagacaa ggtcatcagg
     3601 atcatcgtcg ccactgcggc gggcggtggt tatgacctct acacccggac catggcccgg
     3661 cacttgcgca aatatattcc gggagagccg acaattgttg ttgagaacat gcctggcgca
     3721 ggccacctga tcggggcaaa ctatgtttac aaggtagcta aacccgacgg tctgacgatc
     3781 ggtcatttca tcggttcatt gtttctacag cagcttctcg ttaagccggg cgtcgaattt
     3841 gaagcgctga aattcaattt catcggggtt cccgcgcagg acaactacat gatcggtttg
     3901 tcaaagacga ccggcattac cagtatggaa cagtggctgg cgtcaaagag aattcttaaa
     3961 atcggcggca taggggccgg ttctgccacg gacgatatcc ctaaagtact cgcggccact
     4021 ctcggtctcc ctcttcaagt cgtggctggt tacaagggta cgtcggaagt tcgactggcc
     4081 ttcaattcgg gagagatcga gggaatctgc aacgcatggg aatcctttaa atcgacatgg
     4141 cgcaaggagc tggatgcaaa cgaagtggcg ctggttcttc aggctattcc gacgcgccat
     4201 ccggacgttc cgaacgtgcc tttggctatc gattatgcca agactgacga agcgaaaagg
     4261 atgattcgat cggtcatcca tacaatcgcc ccgacagtac ggccttatgt cttgcctccc
     4321 ggtacaccaa aggaccgcgt tgaaatgttg cgtcgggctt ttatgagcac gatgaaggat
     4381 caggagtttc ttgccgacgc ggctaaagcc aagctcgata tcaatcctct agatggggca
     4441 accctggaga gtaatgttag agaggtcttc aatctcgacc ctgcactgat acctcgattg
     4501 aaggaaattt tgaaataaca gtctttgacc gtttaatgaa tgaacgagga tctagcatga
     4561 cggaatctac aagggttccc cagggcctaa agtcggtgag tgaattgata ctgcgcagct
     4621 attggcatcc agtggcgacg agcagtgagc ttcgcaatgc tccgcttgcg gcgtctttac
     4681 ttgatgagcc aatcgtcgtt tggcggacga aagataaggt agcggcattc cgggacttat
     4741 gcattcaccg gggaactgcc ctatcgctgg gctggatcca agacgataat ttagtttgtc
     4801 cttatcatgg ctggacctac gctcccgatg gcaattgcgt gcggattcct tcgttaccgg
     4861 cgggacacgc aattcctgcc aaagctcgcg ctaccgcgta ttgcaacgtg cgtgaacgtt
     4921 acggcttaat ctgggtgtgt ttagcagagc cgaaagcacc tattcccgag attccagaac
     4981 ttgaagatcc gaattatcac acgttttttc attcgactga catgtgggaa accggcgccg
     5041 cgcgcatgat cgaaaacttt attgacactt cccacttccc ttatgttcat cctggtatca
     5101 atgctactcc cgatgacccg gtgatccctg agttcgaggt gaagcagaac ggtttcgagc
     5161 taagttttga gacgaagttt actgctcccc caagcggggc atttcgaggg ccgtccgcgt
     5221 tagcctcgta cagtgcattt accgagggca ggcgtcagta cagggttgtc ctgccattca
     5281 cggcccaggc tattcgtcca atggagggag cccggcgtca gctagtatcg atcatcgccg
     5341 cgcctatttc ggcaaagcgc atgcgttact acaccttctc ttcccgcaac tttgcattgg
     5401 accaaccgga tgatgcgttt cgagaactta tacaagttat ttttgcgcag gaccgcaaga
     5461 tcgtcgagag ccagcgccct gaagagttgc ctttggattt aa
//
LOCUS       DASUVS010000195        17346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1173323, whole genome shotgun sequence.
ACCESSION   DASUVS010000195 DASUVS010000000
VERSION     DASUVS010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 17346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17346
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173323"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="VF182_10845"
     CDS             <1..1014
                     /locus_tag="VF182_10845"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX6437618.1"
                     /translation="MGIVVMALIPQQSARTAIISQMLMNLSESLGYKSPSKASTGLFV
                     ASFLGLGQLGFLFLTGSTTSLIAWGLLPEEVRAQFNWGYWFYAASVPTLTVIVIVISA
                     TLILYKPESKARVSYKMVQTQLQILGPLSRHEWITLGVLCFTLAGWLSKSYHDIDGAW
                     IALIALCVLINTGVLGWGMMKKGIDWEMLLYMGATLSIPALLTQAKIDQWLVGMFAPV
                     ILPFTNSPALCFTVIILMAIAVKLAFTSFLTVVALTVALLPLASELGISPWVMAMIVL
                     MGSEVWFLRFQVDWHTMASATTEGKGFSYPMMYRINPVYAVAYVLAAIVAIPWWRHLG
                     LIG"
     gene            complement(1084..1863)
                     /gene="phnC"
                     /locus_tag="VF182_10850"
     CDS             complement(1084..1863)
                     /gene="phnC"
                     /locus_tag="VF182_10850"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019378558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEX6437619.1"
                     /translation="MIILEIENLQKSYDNNIPVLCGINLAVNRGEFVGIIGLSGSGKS
                     TLLRCINRLIEPSSGHIRVPAVLITRNGAGNLVDVVRLNQFELRRLRRKIGMIFQQFN
                     IVKRLSVIENVLSGGLGYQSPLRSTLRSFTRDEKRKALTNLHRVGLLDHAYRRADELS
                     GGEQQRVAIARTLMQEPALILADEPVSSLDPKLSSIVLDILKRVCQEDGITALVSLHT
                     LELTREYAARVVGLKNGRVSFDGTCDELTAPVVQAIYHNEL"
     gene            complement(1860..2777)
                     /locus_tag="VF182_10855"
     CDS             complement(1860..2777)
                     /locus_tag="VF182_10855"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6437620.1"
                     /translation="MLASSISRLSIGLFLSLFLVACGHSSKSPRVLRVGFVPAEDAQQ
                     VMQNAQPIVDILEKELGMEIQPFVAADYTGVVEALRVNKLDVAFLTPASYVLAKNEAD
                     VKVVLKSERKGIASYYAAIITRADSGIQTLEGLRGKTFAFGDALSTTGNIFPRKMFKE
                     RGIDPVRDFKQILYSGGHDATVLAVLNGKVDAGATYANSPDGEDTAWMRYLKDPEDVK
                     KIRAIAFSEPIPADNLVINGKLDEAIAKKVEEIFLQLSREPKGKQMMRDLYQIDGFVP
                     ATDRDYDSVRQAFDIAGIPLKTSLQRKQP"
     gene            complement(2850..3707)
                     /locus_tag="VF182_10860"
     CDS             complement(2850..3707)
                     /locus_tag="VF182_10860"
                     /inference="COORDINATES: protein motif:HMM:NF045274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6437621.1"
                     /translation="MHMLAQSFGFWTLIIILASPGWAAEVDEPISPRDAKNACPQCRN
                     DSVEIAQLHKTADRLYAQFKPKEAANELLKILRVDDQNFEALIKLSRAYIDLGDMIPE
                     SGSDWKETRLKDYRTAEDYARRAIKANPNSTWGYFYLGASLGVTAVVSPVARQVELAE
                     EIRVALEKSLALDPNNGFAYHAYGVWHRKVSEIGKMSRMFASVLYGRSLPQGSLEKSV
                     EYLKKAIAINPTVIVSRLELANTYIAMEDFPAARTMLSSVRNLPIQFSDDAKHKQKAQ
                     ELLEEVASR"
     gene            complement(3714..4085)
                     /locus_tag="VF182_10865"
     CDS             complement(3714..4085)
                     /locus_tag="VF182_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX6437622.1"
                     /translation="MADTREPTLRVTMLPRDTNAHGTIFGGIILSHIDLAGGVAASRQ
                     ARRNFVTRAMREVEFIAPVYVGDVVSFYTSIQRQGTTSLTVRVEVEAERLENPRQTVR
                     VTAAEVVYVAVDGAGKPLSIA"
     gene            complement(4128..5045)
                     /locus_tag="VF182_10870"
     CDS             complement(4128..5045)
                     /locus_tag="VF182_10870"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX6437623.1"
                     /translation="MSLKTEKIWLDGELVRWEDATVHVLTHALHYGTAYFEGIRCYEL
                     GDGRSAVFRLPEHLRRLWDSGNILGFPLPYSVEQLTQATLQVVGANRLKECYIRPLAF
                     VGIGDLGLYAPNNPVHVSIAVWPWGAYLGDEGLKNGIRAKVSSFTRHHVNVMMTKSKA
                     AGNYINSVLAKHEVKKAGYDEAIMLDAEGYVSEASGENIFIVRDRKVKTTPLTSILPG
                     VTRDSIITLARGKKYQVIEERFTRDELYTADEAFLTGTAAEVTPIREVDDRRIGSGRR
                     GPITEDIQASFFEVVKGRQTEYSSWLTYL"
     gene            complement(5170..5439)
                     /locus_tag="VF182_10875"
     CDS             complement(5170..5439)
                     /locus_tag="VF182_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX6437624.1"
                     /translation="MTKSELIDSVAKTANISKRAAGEAVDATFVGISKAIKKSKRFQI
                     PGFGTFTVRSRKARKGRNPQTGDVINIKASRTVGFKPAPNIKKGL"
     gene            complement(5533..6468)
                     /locus_tag="VF182_10880"
     CDS             complement(5533..6468)
                     /locus_tag="VF182_10880"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX6437625.1"
                     /translation="MISPRCAFIDIGTNTILCLIAELRDGGRFRVLEDLAEITRLGHG
                     VDRVGLISREGEQRSSAVLRRYLDACKTLGVGEVIAVGTSALRDAGNSVEVRQRWEAE
                     LGLGVRVIGGDDEAAYSFLAAQRGLPLGGEELLVIDVGGGSTEFIRGNDSGVRDAFSL
                     NLGSVRLTERFIRSDPVSAEEFAHMTSAIDSELTILGKRLGHLAGASTWVGIAGTFTT
                     LAAVEKKLTRYSHSMVHGSNLTHLEVQRQVRLYRSQTLEDRKMIPGLEPKRADVILAG
                     ACLIDRMMTLYGAPRLIVSDQGVRYGLLHERLDRR"
     gene            complement(6465..7826)
                     /gene="trmFO"
                     /locus_tag="VF182_10885"
     CDS             complement(6465..7826)
                     /gene="trmFO"
                     /locus_tag="VF182_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX6437626.1"
                     /translation="MEKIVRIVGGGLAGSEAAYQLARRGICVELFEMRPRRMTEAHAS
                     GDLGELVCSNSLRSDSLSAPAGLLKEEMRKLDSLIIRTAEIARVPAGSALAVDREIFA
                     RALTAAVEALPGVTVIRQEIEQIPSGIVILASGPLTSPVLAQQVASLLGQEHLYFYDA
                     ISPIVTAESIDMDIAFRASRYDRDGDDYLNLPLTREEYYHFIDALVKAERVPTYSFER
                     FVPFEGCMPIEEMADRGKDTLAFGPMRPVGLVDQRTGKRPYAAVQLRQENRDRTLYNL
                     VGFQTKMTYPEQRRVFAMIPGLARAEFIRLGSLHRNTFIHSPLHLLPTLQWRQRESLF
                     FAGQMTGVEGYIESAATGLLAGINAARLLSAQPLRVPPDTTALGALLRYITDSQRKKF
                     QPMNVNFGLLPPLSGVLRGKAKKEAMSQRALADMAAWVQDLEVKFAEDLNFSAPLAPA
                     PQP"
     gene            complement(7831..10149)
                     /gene="topA"
                     /locus_tag="VF182_10890"
     CDS             complement(7831..10149)
                     /gene="topA"
                     /locus_tag="VF182_10890"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX6437627.1"
                     /translation="MPAKNLVIVESPAKAKTLEKYLGRDYQVKASVGHVVDLPKSKLG
                     VDVKKNFAPDFHIIQAKKKVIDDLKKAARGKEKIYLASDPDREGEAIAWHIADQVAKN
                     HKNVHRVLINEITKKAVQEAIANPLELDRNKFDAQIARRVLDRLVGYQLSPLLWNKVR
                     RGLSAGRVQSVAVRLVCEREKEIRAFTAQEYWSLTALLEGRLPPSFKARLVQWRENKI
                     DNKKFRLENEPMVQEIVKSLDGASWIIGEVEKKERRRYPTPPFVTSKLQQEAARKLGF
                     QPKRTMQLAQQLYEGLELGPEGSVGLITYMRTDSTRVSNDALAAVRQFIAGHYGKNYV
                     PGKPNSYRSKKGAQDAHEAIRPTSLEYTPDRVRPYLRRDAFQLYSLIWDRFVASQMVP
                     AVFDQTSFEIPVREALFRATGQQIKFDGFMKVYIEGRDAPAGKESEDGEEEAEREAQD
                     SDGVLPDLQRGDALSLLSMDPRQHFTQPPPRYTPASLIKELDEKGIGRQSTYAAIISN
                     IIDREYVAQDERRTLAPTDLGFLVTDLLVESFPDILNVEFTAGMEDELDKIEEGKEKW
                     TRAMKRFYTPFSRDLKKAETEMRDVKRQEVPTEIPCEKCGAMMVIKWGRNGEFLACPQ
                     YPECKSTKNFSRDEKGEIKVAEEVQVEESCENCGRPMLLRWGKYGKFLGCSGYPECKT
                     IQPLEKPVDLGIKCPDCGEGNVKERKSRWGKMFYGCDRYPDCRFATWDKPVPGPCPDC
                     NDPILVEKVTKRAGRTRRCRRKECGYSVPLPE"
     gene            complement(10190..11299)
                     /gene="dprA"
                     /locus_tag="VF182_10895"
     CDS             complement(10190..11299)
                     /gene="dprA"
                     /locus_tag="VF182_10895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX6437628.1"
                     /translation="MSRSDHSEHALWLALTRIKGLGCISFKKLAGHFADPTQSLSATA
                     AELSAIEGIDRNVVDGLLAFTQWDEVEQEVQRANQAGVKIVPFGSAIYPARLRSIADP
                     PACLYVKGEIRQEDDKAVAVIGTRSASDYGRRVARDLCRGLASLGFTVISGMARGIDG
                     VAHDAALTAGGRTIAVLGSGVDRAYPPEHQKLYNRISEHGAVISELPLGAPPLAFNFP
                     ARNRLISGLSAGVVVVEATEKSGSLITAAIALEQGREVFAVPGEVGSSRSRGAHRLIR
                     QGAKLVENVDDILEEIAPQLVATHRPSVTRTLPVEAQPETHKIFELLQERSLHIDEVI
                     EASGISAPKVSQILLELELQGFLRQLPGNRYSAEC"
     gene            complement(11741..13120)
                     /gene="der"
                     /locus_tag="VF182_10900"
     CDS             complement(11741..13120)
                     /gene="der"
                     /locus_tag="VF182_10900"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX6437629.1"
                     /translation="MVEPAPHQNSLEPKLPILAIVGRPNVGKSTLFNRLVGERKAIVH
                     DLPGVTRDRNYGAAEWSGKKFLLVDTGGLDSEAEDGLDERVQEQSRIAMAEADAILFL
                     FDGKGGLNPIDQQAIKQLRKTKKPVFFAVNKLDTLKKQDNLYEFYSLGLDRLFPLSAE
                     HGIGVPDIMNEIVSLFPDEPEVDDRVEEEKPQEPLCIAIIGRPNAGKSTLTNRLLGFE
                     RCVVHSKPGTTRDALDTPFELNGLPCILVDTAGMRRKARIDDRVERFSVQRALRVVDR
                     GDLIIHVIDAAEGVTDQDAQILAYAFQRGKALLLAINKWDLPAKDDKDVEAYRAEVYY
                     NLSFLEHVPVTFVSAVTGFGVRKMLDSAGRLLKAYQRKISTSALNQALQTIVKGHPAP
                     LNQGRPVKFYYGTQTGIRPPTFTLFVNTPSGVGEGYRRYLIHQMRDSLGLEHTPIKLF
                     LRARREERS"
     gene            complement(13122..14045)
                     /gene="era"
                     /locus_tag="VF182_10905"
     CDS             complement(13122..14045)
                     /gene="era"
                     /locus_tag="VF182_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX6437630.1"
                     /translation="MTKESPKNKRGKEPAFRSGYIAIVGRPNVGKSTLLNSLLGEKVA
                     IVSPKPQTTRNRITGIKTSSRSQIVFLDTPGIHQARSLMNRRMVEIALQSLHEVNGVL
                     WLLDSHGRVGLEDERIAATLRGVKSPVFILLNKIDLVSKGRLLPLIQRCAELLPGKEI
                     IPISALKGENLPLLLDLVETGLPEGPKFFPEGEFTDQSERFLAAEIIREKVFLLTHEE
                     IPYGVGVSIDEFTEKEEKNLIVIQATIHTERESHKPILIGKRGSMLKEIGQSAREELE
                     RLLGCKMYLELFIRVDQGWTQNPYALTEMGL"
     gene            14149..14772
                     /locus_tag="VF182_10910"
     CDS             14149..14772
                     /locus_tag="VF182_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437631.1"
                     /translation="MKKRLTRRAQEMIARALPHFPELAGRTITVGYTRKHLGSASIIY
                     RGGKIARLIIRLKVRKLTYQTIGHELTHLVQALVRGDRPHIDSIDLPKVPYGEKPCDI
                     WTLARHELFCDDPPTYIKMPRILREYWLDYAEAVRRLCIAAIERRITQRLYIQWLEIE
                     IAKLARTAPRRRTFPAQLILPFADSPTSTERSSSIDDGVHFSQFFSS"
     gene            complement(14725..15663)
                     /locus_tag="VF182_10915"
     CDS             complement(14725..15663)
                     /locus_tag="VF182_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6437632.1"
                     /translation="MDYRHLGNAGVRVSVIGLGANRFGSKEVSQVEVDRIIDQALELG
                     VNFFDTANVYNEGRSEETLGHALKGRMDEVVLASKFSFPRKTGPNSWGASRYQLMQAL
                     DASLRRLQTDHIDLYYTHRWDASTPIEETLRALDDVIRMGKVRYIGASAYASWQLAHA
                     NVLAEMKGWSSFVVLQSEYNLLRRDVEREVLPYCRAHQVGFVPYYPLAGGFLTGKYEQ
                     GQPAPAGSRGATARNVQEFMTEANYDRVGRLTNWAKARGRGVNELAQAWLLAQAPVCS
                     VISGATKAEQVVSNVRACDWQLGAEELREVDAIINR"
     gene            complement(15692..16057)
                     /locus_tag="VF182_10920"
     CDS             complement(15692..16057)
                     /locus_tag="VF182_10920"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX6437633.1"
                     /translation="MKKVYCAKDPLMIGHLRNVLGTYGITCITKNLDLSAAAGELPPI
                     DCWPELWVEDEQLVRAKSILKKTMAPLKSVKKPWTCGGCAEEIEGQFTECWKCGRSRS
                     REPTRRDNVVSLSEFQRNR"
     gene            complement(16077..16415)
                     /locus_tag="VF182_10925"
     CDS             complement(16077..16415)
                     /locus_tag="VF182_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015126918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOPA 4,5-dioxygenase family protein"
                     /protein_id="HEX6437634.1"
                     /translation="MSQREIKGYHAHIYYDPASREAATRVRAGLANFNVQLGNWHDEP
                     VGPHPKAMYQALFANDEFSRVVPWLMLNRQGLTVLVHPSTGDSYGDHLERSLWLGEVL
                     KLREEVLRRA"
     gene            complement(16998..>17346)
                     /locus_tag="VF182_10930"
     CDS             complement(16998..>17346)
                     /locus_tag="VF182_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437635.1"
                     /translation="PSQRGIHLLTLYAEGEPGHAGQPVGTIPYIAPYPKEYRELKPNT
                     ALLSRLAEETGGEMIDADKLDNGVARLYTPTPGKGRRGQETWWPFAGFGLLLFLVDLV
                     IRSWPRRSAQIQA"
BASE COUNT         3782 a         4974 c         4520 g         4070 t
ORIGIN      
        1 atgggcattg tcgtcatggc attgatcccg cagcagagcg ctcgcaccgc gatcatcagt
       61 cagatgctga tgaacttgtc cgagagtctt ggttataaga gtccgtccaa ggcttcgacc
      121 ggtcttttcg tcgcgagctt cctgggcctc ggtcaactcg gttttctatt tctgaccggt
      181 tcaaccacga gcctgatcgc atggggcctg ctgcccgagg aggttcgagc gcaatttaac
      241 tggggctact ggttttatgc cgcatcggtg ccgacgctta cggtgatcgt gattgttatt
      301 tcagccaccc tgattcttta caaacccgag tctaaggcgc gtgtttccta caagatggtc
      361 caaacccagt tgcagattct cggccctcta tcacgccatg aatggattac cctgggagtc
      421 ttgtgcttca cgctggcggg ctggctcagc aagtcgtatc acgatatcga tggcgcttgg
      481 attgcgctga tcgcattgtg tgttttgatc aataccggcg tgctcggttg gggtatgatg
      541 aaaaagggca tcgactggga aatgctcctc tacatgggcg cgactctgag cattcccgcg
      601 ctgcttaccc aagcaaagat cgaccagtgg ctggtcggta tgtttgctcc cgtaatcctg
      661 ccgtttacga attcgccagc gctctgtttc accgtcatca tacttatggc aattgcggtg
      721 aagctggcct ttaccagctt cttgactgtc gttgccttga ctgtggcgct tttgccgctc
      781 gcctctgagc tgggcatcag tccatgggta atggctatga tcgttctcat gggttcagaa
      841 gtttggtttc ttagatttca ggtggactgg cataccatgg caagcgccac aaccgaggga
      901 aaaggattta gctatcctat gatgtaccgc attaatccgg tgtacgccgt ggcatacgta
      961 ttggcggcta tagtcgcgat cccgtggtgg cgccacttgg gtttgatcgg ttgagccggc
     1021 tcgtccgcgt ggacgaatat taaattcagc tcacagcgga ccacagctag cgacctcatg
     1081 aggtcaaagc tcgttgtgat agatggcctg caccaccggc gcagttagct cgtcgcaggt
     1141 tccgtcgaag gagacccggc cattcttaag tccgacgacc cgtgcggcat attcacgggt
     1201 tagctctaaa gtgtgcaagc tcaccaaggc tgttatgccg tcttcctgac agactctttt
     1261 gagaatatca agaacgatgg aagacaactt cgggtcgagg ctcgaaaccg gctcatcggc
     1321 tagaatcagc gccggctcct gcatcaaagt tcgagcgatg gccacccgct gctgttcacc
     1381 gccgctgagt tcgtcggcgc ggcggtaggc atggtccagc aatccaacgc ggtgaagatt
     1441 ggtcagggcc ttgcgctttt catcacgggt aaaacttcgc agcgtgctgc gcaacggcga
     1501 ctgatagccc aagcccccgg acaaaacatt ttcgatgacg gacaaacgct tgacgatgtt
     1561 gaactgttga aaaatcatgc cgatcttgcg tctcagccgt cgcagttcaa attgattcaa
     1621 tcgcacgaca tcgaccaggt tgccggctcc atttcgtgta attagcacag cgggcactcg
     1681 gatatggccg gaactgggct cgatcagacg gttgatgcag cgcagcaaag tcgactttcc
     1741 cgagccgctg agcccgatga tgccgacgaa ctcgcctcga ttaacggcta aattgatgcc
     1801 acagagcacc ggtatattgt tatcgtagga tttttggaga ttctcgattt ctagaatgat
     1861 cacggctgct ttctctgtaa acttgttttc agcgggatac cagcgatgtc gaaggcctga
     1921 cgcacagaat catagtctcg atcagtagcg ggaacgaacc cgtcgatctg ataaagatcg
     1981 cgcatcatct gcttgccttt aggttcgcga ctcagctgca aaaaaatttc ttccactttt
     2041 ttggcaatag cttcatcgag cttgccgttg atcactagat tgtcagccgg gatcggctcg
     2101 gagaaagcga tcgcgcggat cttcttcacg tcttcgggat ccttcaaata gcgcatccac
     2161 gcggtatcct cgccgtcggg cgaattggcg taggtcgccc cagcatcgac tttcccgttg
     2221 agcacggcaa gcacggtggc atcgtgcccg cctgaataca gtatttgttt gaagtcgcgc
     2281 accggatcga taccgcgttc cttgaacatc ttgcgcggga aaatgttgcc agtggttgat
     2341 agcgcgtcgc cgaaagcgaa agttttgccg cgcaggccct cgagcgtttg gatgccgctg
     2401 tctgctcggg tgatgatcgc cgcataataa gaagcgattc ccttgcgctc cgacttaaga
     2461 acgactttca cgtcagcttc attcttcgcc aatacatagg aggccggggt caagaaggca
     2521 acatcgagtt tgttaacccg gagcgcttcg accactccgg tatagtccgc cgcgacgaag
     2581 ggctgaatct ccatacccaa ttccttttcc agtatgtcga cgatgggctg cgcattttgc
     2641 atcacttgtt gagcatcttc cgccggtacg aagccaaccc gaagcacccg gggcgatttg
     2701 cttgaatgac cacatgcaac cagaaaaagg cttaagaata atccgataga aagtcgtgat
     2761 atcgaagatg cgagcatgtt gaggcttatc gtgacagttc accgctgggg gcgcaaatcc
     2821 ctgatcatgg gcgaagcatc cgagcggctc tagcggctcg caacttcttc caacaactct
     2881 tgagctttct gcttgtgctt ggcgtcgtcg gagaattgaa taggtaaatt ccggactgag
     2941 gagagcatcg tgcgggcagc cggaaaatct tccatggcga tgtaagtatt tgccaattca
     3001 agccggctta cgatcacggt tggattgatt gcgattgcct ttttgaggta ctccactgat
     3061 ttttccaggc tgccttgcgg tagagaacgg ccgtagagaa ccgaagcgaa catgcggctc
     3121 atttttccga tttcagagac ttttcggtgc caaaccccat aagcatggta cgcaaaccca
     3181 ttgttcggat ccagcgcaag cgatttttcc aacgcgacgc ggatctcctc cgccaattcc
     3241 acctgtcgtg caaccgggga aacgacggcc gtgactccta acgaagcgcc caggtaaaaa
     3301 taaccccagg tcgagtttgg attggctttg atggctcggc gagcgtaatc ttctgccgtt
     3361 cgataatcct ttagacgtgt ctccttccaa tcggacccag actcggggat catgtcgcca
     3421 aggtcgatat acgcgcgcga cagtttgatg agagcttcaa aattctgatc gtccacgcgc
     3481 aaaattttca gcagctcatt ggcggcttct ttgggtttga attgagcata cagccgatcg
     3541 gcagtcttat gcagctgcgc aatctcaaca ctgtcgttgc gacactgggg gcatgcgttt
     3601 ttggcatccc gcggtgaaat gggctcatcg acttccgcag cccatcccgg cgatgccaga
     3661 atgatgatca gagtccaaaa gccgaagctc tgcgccagca tatgcatcta tgcttatgct
     3721 atggatagcg gctttccggc cccatctaca gcgacgtaca cgacctcagc cgccgtgacc
     3781 cggaccgttt gccgcgggtt ttcgagtctt tccgcttcaa cttccacccg gactgttaac
     3841 gaggtcgttc cctgacgttg tatcgaagta taaaaactca ccacgtcccc gacgtacacc
     3901 ggcgcgataa attcgacttc ccgcatcgcg cgagtaacga aatttcgacg ggcctgacgc
     3961 gacgccgcca ctcctccggc aagatcgata tgactgagaa tgatgccgcc gaagatggtt
     4021 ccgtgcgcat tcgtgtctcg cggaagcatc gtcacccgca gggtcggttc acgagtatca
     4081 gccatccgca gccacccgat aatttgataa agattagtct gcgacaacta aaggtaagtc
     4141 aaccagctcg aatactcggt ttgtctccct ttcaccactt cgaaaaaaga ggcttgaatg
     4201 tcctcggtga tcgggcctcg ccggccgctc ccgatccgtc tatcatcgac ctcgcgaatg
     4261 ggagtcactt cggccgcggt tccagtaaga aacgcctcat cagcagtata gagctcatcg
     4321 cgtgtaaacc gttcctcgat gacttgatac tttttaccgc gggccagagt gatgatcgaa
     4381 tcgcgagtca cgcccggcag aatcgaggtc agcggtgtgg ttttcacctt acgatcgcgc
     4441 acgatgaaaa tgttttcgcc actcgcttcg gaaacgtaac cctccgcgtc gagcataatc
     4501 gcttcgtcat accctgcctt tttaacctca tgtttggcaa gcacggagtt gatgtaattg
     4561 ccggcggcct tacttttggt catcatcacg ttgacgtgat ggcgagtgaa agacgagact
     4621 ttcgcacgta tgccgttctt gagcccttca tctccaagat aggctcccca tggccaaacg
     4681 gcgatgctca catgaactgg gttattcgga gcgtacaaac ccaaatctcc aatcccaacg
     4741 aaagcaagcg gccggatgta acactctttg agccggttag caccaacgac ttgaagtgtc
     4801 gcttgggtca gctgttcgac tgaatagggc aaggggaacc cgagaatatt acccgagtcc
     4861 caaaggcggc gcagatgttc cggcaaacga aataccgcgg aacggccatc gcccaattcg
     4921 tagcagcgaa tcccttcgaa atacgctgtg ccgtaatgta acgcatgtgt cagcacatga
     4981 accgtcgcat cctcccagcg caccagctcg ccatcgagcc atatcttttc ggtcttgagg
     5041 gacatatcta aactttcgtg atctgagcca ttgctgcatt agtctacgtg cccagtacag
     5101 taccagcaag ggctctccaa aaaaaaggcc accacgaggg tggtggcccg gcatggatcg
     5161 gcaatgatac tacaaacctt ttttaatatt cggcgccggt ttgaacccga ccgtgcggct
     5221 ggctttgata ttgatcacgt cgccggtttg aggatttctc cccttgcggg ctttgcgaga
     5281 tcgtacggta aaggtgccga agccgggaat ttggaatcgt ttactcttct tgatcgcctt
     5341 ggagatacct acgaaggttg catccactgc ttcccccgct gctcttttgc tgatgtttgc
     5401 ggttttcgcc actgagtcga tgagttcgct tttcgtcatt gtctttcgct cctccttgca
     5461 agctgatcga tcggtatgat cgtccaaagc aggtcgaaat aatcatggat tcatcctgaa
     5521 atgtcaaata ctttatctcc ggtccaagcg ctcgtgcagc agcccgtaac gaaccccctg
     5581 atcgctgaca atcagccgcg gcgcgccata caatgtcatc attctgtcga tcaaacaggc
     5641 gccggcaaga atcacatcgg ctcgtttggg ttcgaggcca ggaatcattt tacggtcttc
     5701 gagagtttga cttcggtata atcgcacctg ccgttgaacc tcaaggtgcg tcaagttgct
     5761 gccatgtacc atgctgtgcg aatatcgcgt caacttcttc tccacggcgg ccaaggtggt
     5821 gaaggttccc gcgattccaa cccaagtcga agccccagca agatggccca atcgtttccc
     5881 caaaatcgtc aactcgctgt cgatggcgct cgtcatatgg gcaaattctt cggcgctaac
     5941 gggatcggaa cgaataaacc gttcggttaa tcggaccgat ccgagattga ggctgaaggc
     6001 atctctaaca ccggaatcat tgccgcggat aaattccgtg ctgccgccgc cgacgtcgat
     6061 gacgagcagc tcctctccac ccaacggaag tcctctctgc gctgccagga aagaataggc
     6121 ggcttcatca tcgccgccaa tgacacggac gccgagtccc agctcggcct cccagcgttg
     6181 ccgtacttca acactgttgc cagcatcgcg aagagcgctg gttccaaccg ctatcacttc
     6241 gccgacaccc aacgttttgc acgcgtccaa gtaacggcgc aacacggccg agcttctctg
     6301 ctctccttct cggctgatca atcccacccg gtcgactcca tgccccagac gagtaatctc
     6361 cgcgaggtct tctagcactc tgaaacggcc gccatctctg agctcggcta tcagacacaa
     6421 aatcgtattg gtcccgatat cgatgaaagc acaacgcggc gagatcatgg ttgcggcgca
     6481 ggagcgagcg gcgcggagaa gtttagatcc tcggcgaatt ttacttcgag atcttggacc
     6541 caagcggcca tatcggccag cgccctttgc gacatcgctt cctttttggc cttgccccgc
     6601 aaaacgccgg ataacggcgg caacaggccg aagttgacgt tcatcggctg aaattttttt
     6661 cgctgcgaat cagtgatgta acgcaacaga gcgcccagag cggtggtgtc gggcgggacc
     6721 cgaagcggtt gagcggacag caatcgcgcg gcatttattc cggctagcaa gccggttgcg
     6781 gcagattcga tatagccttc gacaccggtc atctggccgg caaaaaacaa actctcgcgc
     6841 tggcgccatt gcaaagtggg cagcaggtgg agcggcgaat gaataaacgt gtttcggtgc
     6901 agactgccca ggcgaatgaa ttcagcccgc gccaagcctg ggatcatggc gaaaacgcgg
     6961 cgctgctcgg ggtaagtcat tttggtttgg aacccgacca ggttgtacaa cgtccgatcg
     7021 cggttttcct gacgcaattg gaccgccgca tacgggcgct ttccggtgcg ttgatcgaca
     7081 agtccgactg gccgcatcgg accgaacgcc aaagtgtcct tgccgcggtc ggccatctct
     7141 tcgatgggca tgcagccttc aaacggcacg aagcgctcga aactataggt cggcactcgt
     7201 tctgctttca ccagcgcatc gatgaaatgg taatactcct cgcgcgtaag cggcagattc
     7261 aggtaatcgt cgccatcccg atcatatcgt gaggctctga acgcgatgtc catgtcgatg
     7321 gattcggcgg ttacgatcgg tgaaatcgca tcataaaagt agagatgttc ttggcccagg
     7381 agcgacgcca cctgctgtgc tagaaccggc gaggtgagcg gcccgcttgc cagaataacg
     7441 atgcccgaag ggatttgctc gatttcttgg cgaatcaccg tcacgcccgg tagtgcttcg
     7501 acggcggcgg tcagggcacg cgcgaagatc tcgcgatcca ccgctaatgc cgatccggcg
     7561 ggcacccgcg cgatttccgc ggttctgata atcaaagaat cgagcttgcg catctcttct
     7621 tttagtagac cagcgggggc ggataacgaa tccgagcgca gcgagttgct gcacaccagt
     7681 tcgcccaaat cacctgaagc gtgcgcctcc gtcatacggc gcggccgcat ctcgaacaac
     7741 tcaacgcaga taccgcgtcg cgcgagctga taggcggctt cggaaccggc caatccgccg
     7801 ccgacgatgc gaacgatttt ttccatcgat ttattccggg agcggcactg aataaccgca
     7861 ttcttttctg cggcagcgcc tggtgcggcc ggcccgcttg gttacctttt cgaccaagat
     7921 cggatcgttg caatccgggc atggccctgg caccggctta tcccaagtag cgaacctaca
     7981 atccggataa cgatcacagc cgtaaaacat cttcccccag cgcgatttac gttccttcac
     8041 gttgccttcg ccgcagtccg ggcacttgat gccgagatct accggcttct ccagaggctg
     8101 tatggtcttg cattcgggat aaccgctgca gccgagaaat ttgccgtact tcccccaacg
     8161 cagcaacatc ggcctgccgc agttttcgca gctttcctcg acctgcactt cttcagcaac
     8221 tttgatttcg cctttctcgt cccggctaaa attcttggtg cttttgcatt cagggtattg
     8281 cgggcaggct aagaactcgc cgttgcgacc ccatttgatc accatcatcg cgccgcattt
     8341 ttcgcacggg atttccgtcg gcacctcttg ccgcttgacg tcgcgcatct cggtttccgc
     8401 tttcttgaga tcgcgcgaaa agggggtgta aaagcgcttc atcgctctgg tccacttttc
     8461 tttcccctct tcgatcttgt ccagttcgtc ttccatcccg gcggtaaact caacattgag
     8521 aatatccgga aaactttcca ccaacagatc ggtcaccaag aagcccaggt cggtgggcgc
     8581 taaagtgcgt cgttcatcct gagcgacata ttctcgatcg atgatattgg agatgatcgc
     8641 ggcataggtc gactgccgcc cgataccttt ttcgtccagc tctttgatca gggaggccgg
     8701 cgtgtagcgc ggcggaggct gagtaaaatg ttggcgcgga tccatggaaa gaagcgacag
     8761 cgcatcccct ctttgcagat cgggcaacac accatcgctg tcctgagcct cgcgttccgc
     8821 ctcctcttcg ccgtcttcgc tttctttgcc ggccggtgcg tctctccctt cgatgtaaac
     8881 tttcatgaag ccatcaaact taatctgctg tccggtggca cgaaacagcg cctctcgcac
     8941 cggaatctca aaactagtct gatcaaagac cgccggcacc atctggctgg caacgaatcg
     9001 gtcccaaatc aacgagtaga gttgaaaagc gtcgcggcgc aaatacggcc ggactcggtc
     9061 gggcgtgtac tccagcgaag ttgggcggat cgcctcatga gcgtcctgcg ctcccttttt
     9121 cgagcgatag ctgtttggtt taccgggaac atagtttttt ccataatgcc cggcgatgaa
     9181 ttggcgcaca gcggcgaggg catcgttgga cacgcgggtc gaatcggttc gcatgtaagt
     9241 aatcaaacca acggaacctt ccggcccgag ctcaaggcct tcgtagagtt gttgggccaa
     9301 ttgcatggtt cgtttgggct gaaaacccaa ctttcgcgct gcctcttgct gcagtttgct
     9361 ggtgacaaac ggcggcgtcg gatagcgccg tcgctccttc ttctcgactt cgccgatgat
     9421 ccatgatgcg ccgtcgagag atttgacgat ctcctgcacc atcggctcgt tctcgagtcg
     9481 gaatttcttg ttgtctatct tgttttcacg ccattgaacc agccgggctt tgaacgatgg
     9541 cggcaggcgg ccttcgagaa gagccgtcaa agaccagtac tcttgggcgg taaacgcccg
     9601 tatctccttc tcacgctcgc aaaccagccg caccgccacc gactgtacgc gccccgcgct
     9661 gagtccgcgc ctgactttgt tccacaagag cggactcagt tgataaccga ccagtcgatc
     9721 gagcacccgt cgcgcgattt gcgcgtcgaa cttgttgcga tccaactcga gcggattcgc
     9781 gatcgcctcc tggaccgctt tcttggtgat ttcgttgatc aacacgcgat gaacgttctt
     9841 gtgattcttg gccacttgat cggcgatatg ccacgcgatt gcctcaccct cgcgatcagg
     9901 atcggacgca agatagatct tctccttgcc tcgggccgcc ttttttaaat cgtcaataac
     9961 ttttttcttt gcctgaatga tatggaagtc cggcgcaaaa tttttcttga cgtcgacacc
    10021 gagtttgctc ttgggcagat cgaccacatg ccctacggaa gctttgactt gataatctcg
    10081 gcctaaatat ttttccaatg tttttgcctt ggcgggagat tcgacgatga ctaaattttt
    10141 tgctggcata accctcaacc ccatccttgt tgtctaacgt atgaacatat caacattccg
    10201 ctgaatagcg attgcccggg agttgcctga gaaacccttg caactcgagt tccaaaagga
    10261 tctgcgacac tttcggagcc gatataccgc tggcttcgat gacctcgtcg atgtgcaatg
    10321 agcgctcctg aagcaattca aatattttgt gcgtttccgg ttgagcctcg accggcaacg
    10381 ttcgcgtcac cgatggacga tgggtcgcga ccaactgagg cgcgatctct tcgagaatgt
    10441 cgtcgacgtt ttcaaccagc ttcgcacctt ggcgaatcaa acgatgcgcg ccgcgactgc
    10501 ggcttgagcc cacttctccc ggtacggcaa aaacttctct cccttgttcc agcgcgatcg
    10561 cagccgtgat caacgaaccg ctcttctcag tggcttcgac aaccaccacg ccggctgaga
    10621 gaccgctgat caggcgatta cgcgccggga aattgaacgc gagcggcgga gcaccgagcg
    10681 gcaactcgga aatcacagcg ccgtgctcgc tgatccggtt ataaagcttc tgatgctcag
    10741 gcggataagc tcggtcgacc ccggatccca ataccgcgat ggttcggccg ccggcggtga
    10801 gcgccgcatc gtgagccacg ccgtcgatgc cgcgagccat accgctgatt accgtaaaac
    10861 ccagagaagc tagaccgcga caaaggtcac gcgccactcg ccgaccgtaa tcactcgcac
    10921 tgcgcgtgcc aatcaccgca acggctttat catcctcctg ccgaatctct cctttgacat
    10981 aaagacaagc aggcggatcg gcgatgctcc gcaaacgagc tggatagatc gcgctgccaa
    11041 atggaacgat ttttactccc gcctggttgg cgcgctgtac ctcctgctca acctcatccc
    11101 attgcgtaaa ggctagcagc ccatcgacca cgttgcggtc gatgccttct atcgcagaga
    11161 gttcagcggc tgtagccgat aacgactggg tgggatcggc gaagtggccg gccagctttt
    11221 taaaactgat acagcctaac cctttgatcc gcgtcagcgc aagccataaa gcatgttcac
    11281 tgtgatccga cctggacaaa ccacacctct cgagaaagtt acgcatgcct gaatcacatt
    11341 cgttccaggt ttgtcaatgg gtcatgcgag aacgcgggac agcagaccta aaaaggatat
    11401 gcgaaatagc caagcaacgc tacaactcgc ttttggcaat ccgctacaag ctcgaacggc
    11461 gattctggta aacgtatagt ttgtttattt tgccaacctt tgtctaatca acagctttga
    11521 tacttattct ccaaccgagt ttgctgcaaa tgagaattaa ccgactaatc agacgctttt
    11581 ctgcagaaat cgtacaaacg cgagcggtgg ctgcgccgcc ggaagatgat gccagggata
    11641 tctgagcacg tcagcggagg aacttcaggc ttgcggcaaa ggtatcaatt gaatgaacgc
    11701 gcctgcggac agccaaatga aatctagcat ctcgggatag ctagcttcgt tcctcgcgtc
    11761 gagcgcgcag gaagagctta atcggcgtgt gttccaatcc taagctgtcg cgcatctgat
    11821 gaatcaaata tcttcgataa ccttcgccca ccccagatgg cgtattgaca aacagagtga
    11881 atgtcggcgg gcggatgccg gtttgggtac cgtaatagaa tttgacgggc cgtccctgat
    11941 tcagcggcgc aggatgccct ttaacgattg tctgcagcgc ttgattgaga gccgaggtcg
    12001 agattttacg ctgatacgct ttgagcaatc ggccggcgct gtcaagcatt tttcgtacgc
    12061 caaagccggt tacggcggag acgaatgtta ccggaacatg ttcgaggaac gacagattgt
    12121 aataaacctc cgcgcgatag gcttcgacat ccttgtcatc cttagccggc aaatcccatt
    12181 tgttaatcgc cagcagtagg gccttgccgc gttggaatgc gtaggccagg atttgcgcgt
    12241 cctggtcggt gactccttcc gctgcatcga tgacgtgaat gatgagatcg cctcgatcga
    12301 caacccggag cgcgcgctgc acactaaagc gctcaactcg atcgtcgatc ctggccttgc
    12361 gccgcatccc ggcagtatcg acgagaatac aaggtagccc gtttagctcg aatggcgtgt
    12421 ctagcgcatc tcgcgtggtc ccaggttttg agtgaaccac gcagcgctca aagccaagca
    12481 accggttggt gagcgtcgac ttgccggcgt tcggacggcc gataatggcg atgcataggg
    12541 gctcttgcgg tttttcctct tccactcgat cgtcaacttc cggctcatcc ggaaacaggc
    12601 tgacgatttc attcatgata tccggcaccc cgatgccgtg ctcggccgac agtgggaata
    12661 accgatccag gcccaacgaa taaaactcgt acaagttgtc ttgtttcttg agagtatcca
    12721 gtttgttaac cgcgaaaaat accggctttt tggttttgcg gagctgcttg atggcttgtt
    12781 gatcaatcgg gttaagcccc cccttgccat cgaacaggaa gagaatcgca tccgcttcgg
    12841 ccatggcaat tcggctttgt tcctgtaccc gctcgtcgag accgtcttca gcctcactat
    12901 ccagacctcc ggtatcgacc agcaagaatt tcttaccgga ccactcggcc gcgccgtaat
    12961 tgcggtcacg agtaacgccg ggaagatcgt gaacaatagc tttgcgctcg ccgaccagtc
    13021 gattgaagag cgtggacttg ccgacgttgg gccggccgac aatggccaag atcggcagct
    13081 tcggttccaa actattctgg tgcggcgccg gctcgaccat cttataagcc catctccgtt
    13141 agagcgtagg gattctgggt ccaaccctga tcgactcgaa taaacaactc caaatacatc
    13201 ttgcagccca gcaatctttc caactcttcc cgagcgctct gaccaatctc tttgagcatc
    13261 gaacctcttt tgccgatgag aatcggctta tgggattcgc gctcggtgtg gattgtcgct
    13321 tgaattacaa ttagattctt ttcctctttt tcagtgaatt catcgatgct cacgccgacg
    13381 ccgtagggga tctcctcgtg ggtgagcaag aatactttct cacgaatgat ctcggcggca
    13441 agaaatcgct cgctctgatc ggtgaactcc ccctcgggaa aaaatttggg tccctcgggc
    13501 aaacccgttt cgaccaagtc caatagcaaa ggcaggtttt ctcctttaag agcgctgatc
    13561 gggatgattt ccttaccggg cagcagttcc gcgcaacgtt ggattagcgg cagcagcctt
    13621 cccttggaca ccaagtcgat tttgttcaaa agaataaaca ccgggctctt tacgcctcgc
    13681 agagttgcgg cgattctttc gtcctccagg ccgactctac cgtgtgaatc gagcagccac
    13741 agcaccccat tgacttcgtg caggctctgc agcgcgatct cgaccatgcg ccggttcatc
    13801 agcgagcgcg cctggtgtat tcccggggta tccagaaaca cgatctgcga acgggagctg
    13861 gttttaatcc cggtgatgcg attgcgcgtc gtctgaggtt tgggcgaaac gatcgcaact
    13921 ttttcgccga ggagactgtt aagcagggtc gatttgccga cattgggacg gccgacgatt
    13981 gcaatgtacc ccgatcgaaa cgcaggttct ttgccgcgct tgttctttgg gctttccttt
    14041 gtcataagtt tcgatttata agctagatta tggcacgggc caatcaacct tcaaactcgc
    14101 cgatcttcga acaacttgca cagcgtgccg cttaagctca caccattgat gaagaagcgg
    14161 cttacgcgcc gcgctcagga aatgattgcg cgcgctctgc cgcactttcc ggaattagcg
    14221 ggtagaacga tcaccgtggg ttatacgcgc aagcacctgg gcagcgcgag catcatttat
    14281 cgcggcggca aaatcgcgcg tctgatcatc cggctcaaag tccgaaaatt aacttaccag
    14341 acgattggcc acgagcttac ccatctggtg caggcgctgg tgcgcggtga ccgcccgcat
    14401 atcgattcga ttgacttgcc aaaagttccg tacggagaga agccctgcga catctggaca
    14461 ttagcacgcc atgagctctt ctgcgacgat ccacccacct acatcaaaat gccgcgaatc
    14521 ctgcgcgagt attggctcga ctacgccgaa gcggtacgaa gactgtgcat tgcggccatc
    14581 gaacgtcgga tcacccagcg gctatacatt cagtggcttg aaatcgagat cgccaagctg
    14641 gccaggactg cgccccgccg gcgaaccttt ccagcgcagc tgattttgcc gtttgccgat
    14701 tcaccaacgt cgactgaacg gtcatcatcg attgatgatg gcgtccactt ctcgcagttc
    14761 ttcagctcct agctgccaat cacaggctct tacgttgctt acgacttgtt cagccttggt
    14821 cgcgccggag attaccgagc aaaccggcgc ttgcgccagc agccatgcct gcgccagctc
    14881 gttcaccccc cgacccctcg ccttggccca gtttgttaat cgaccgaccc gatcatagtt
    14941 ggcctcggtc ataaactctt gcacgtttct ggcagtggcg ccgcgagagc ccgccggtgc
    15001 tggttggcct tgctcatatt tgccggtaag aaagccgccc gccagggggt aataaggaac
    15061 gaagcccacc tgatgagcgc gacagtaagg caatacttcc ctttcgacat cgcggcgcag
    15121 gagattgtat tcggattgaa gaaccacaaa cgaggaccag cccttcattt cagccaaaac
    15181 gttggcgtga gccaactgcc atgacgcata agccgatgcg ccgatgtagc gcaccttgcc
    15241 catgcgaatg acgtcatcca gcgctcgcaa cgtctcctcg atcggcgtcg atgcatccca
    15301 gcgatgagta taataaagat cgatgtggtc ggtctgtagc cggcgcaaac tcgcatcgag
    15361 ggcttgcatt aactgataac gcgatgcgcc ccaagagttt ggaccggttt ttctcggaaa
    15421 cgaaaatttg ctggccagga ccacttcatc catgcgacct ttcaacgcgt ggcccaaggt
    15481 ttcttcggac cgtccttcat tgtaaacgtt agcggtatcg aaaaaattca ctccaagctc
    15541 gagagcttga tcaatgattc tgtcgacctc gacttggctc acttccttcg aaccgaaacg
    15601 attggcgccc agcccaatga cagaaacacg cacgccggca ttgcccagat gacggtagtc
    15661 catgaaagct ccttcaggtg actagagaga gttatctgtt ccgctggaac tcagagagag
    15721 aaacaacatt atcccggcga gtaggttcac gagatcgact tctgccgcac ttccaacact
    15781 ccgtgaattg tccttcgatt tcctcagcgc agccgccaca ggtccatggc ttcttgacgg
    15841 atttcaaggg cgccatggtt tttttcagga tcgacttggc tctgacaagc tgctcatcct
    15901 caacccagag ctcgggccag caatcgatgg gcggcagttc gcccgccgcc gcggaaagat
    15961 cgagattttt ggtgatgcag gtaataccgt aggtgcccag aacgtttctc aaatggccga
    16021 tcatcagcgg atctttggcg cagtatacct tcttcattag ttttccatta gcaggctcag
    16081 gcgcggcgca acacctcttc tcttagcttc aagacctctc ccagccaaag agaacgctcc
    16141 agatgatcgc cataactgtc gccggtggat ggatggacca gcacggtcaa gccttgccga
    16201 ttgagcatca accaaggaac gacccgggag aactcgtcgt ttgcaaacag cgcctgatac
    16261 attgctttcg gatgaggtcc aacgggctca tcatgccagt tgccaagctg cacattgaag
    16321 ttcgccagcc ctgcgcgcac ccgggtcgct gcttcacggc tagccggatc gtagtaaata
    16381 tgcgcatgat agccttttat ttcccgctgg ctcatacagg cctcctgagc gcttcctatg
    16441 gttgtaccaa atctgcccgc cggattccaa acggagtaga gatcgtggct cataaactcc
    16501 tcgacagttg catgaaagat ccgctgcgga acaatggtgc attaccgaat tggcgaccac
    16561 gttagtagcg attggaaatg gagcacatcg ctccaaccac caacccccgc cggtggctgt
    16621 tttatccggt gcaatccctt ggtatcacgc ctcaaacttt gctcgagggc ggctgtgcgg
    16681 cagagttctt gtccaaacat tcagagcaga tgccggattg cttcttccac aagcctctga
    16741 agaaatgtct ccggcataaa atcctgcgac actgtttgca ggccgcgccg cttaactcgc
    16801 cgaaagttcg cttgcagata ccgcagatta aaggagctat cgagaatgcc atctaagtga
    16861 ttttcctccg gactctctcg aaaaccgtcc tgtcgagttg cttaccagcg ggttcgatga
    16921 tcttctccgc ttcgatatcg cgcgcttgcg cgtttgacaa tctcgcatcg cgggccccga
    16981 caggctcacc acaacgatca agcctgaatc tgcgcggacc tgcgtggcca actccgtatt
    17041 accagatcga caagaaacaa cagtaacccg aaacccgcaa acggccacca ggtttcttga
    17101 ccacgcctgc ctttgcccgg cgtcggcgtg tagagacggg cgacgccatt gtcgagctta
    17161 tcggcatcga tcatttcccc gccggtctct tcggcaaggc ggctcaggag ggccgtgttg
    17221 ggcttcagtt cacggtattc cttcggatag ggagcgatgt aaggaatcgt tcccaccggc
    17281 tgacctgcgt gtcccggctc tccttcggca tagagcgtca gcaaatggat gccgcgctgt
    17341 gatggc
//
LOCUS       DASUVS010000196        15746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1829193, whole genome shotgun sequence.
ACCESSION   DASUVS010000196 DASUVS010000000
VERSION     DASUVS010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 15746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15746
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829193"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VF182_10935"
     CDS             complement(<1..686)
                     /locus_tag="VF182_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6437636.1"
                     /translation="MRIDEAEKSSRTQSRYAETEKRSSSAEYDEAEESFAELRKVDCL
                     DDYVRNHPSAVGLFECALQKALNSAYPTATDAANFHSSQADNCARLFLQRVLYQINRL
                     KLFWFDDLRNYDNERSDYLCQLRDRIERAWQAWELSMLNLAALRREKVRDGLLRRAAL
                     DVSPPASASGLYFRHHVGRVGYRRLLAIASLDGLVEASQLSRTLGGVSNDIHSMLTRL
                     MVEEYGGGRL"
     gene            1573..3675
                     /gene="glgX"
                     /locus_tag="VF182_10940"
     CDS             1573..3675
                     /gene="glgX"
                     /locus_tag="VF182_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX6437637.1"
                     /translation="MKVWPGNNYPLGASYDGAGTNFSIFSEVAERVELCLFDEQGNET
                     RCDLPEVTSLCWHGYLPNIVPGQRYGFRVHGPWAPQEGMRCSPAKLLLDPYARAIVGQ
                     VDWNEAIFSYAFDDPDGPLNQADSAPFMPKSVVINPFFDWGNDRHPRTRWSETIIYET
                     HVKGFSKRFSDIPEELRGTYAGLGHPKAIEYLTQLGISAVELMPVHQFVHDSHLLERG
                     LRNYWGYNSIGYLAPHNEYSSGGQTGQQVQEFKQMVKALHEAGIEVILDVVYNHTAEG
                     NHLGPTLSFKGLDNAAYYRLMNDDRRFYMDYTGTGNTLNMRHPHVLQLIMDSLRYWVL
                     EMHVDGFRFDLASTLARGLHDVDRLSAFFDIIQQDPVISQTKLIAEPWDVGEGGYQVG
                     NFPPLWLEWNGKYRDCVRDYWRGQDQTLGEFAFRITGSSDLYEGTGRRPYASVNFITA
                     HDGFTLNDLVSYNDKHNEANGEENRDGESHNRSWNCGAEGPTDDPAVNALRNQQKRNF
                     LSSLFLSEGIPMLLGGDEIGRTQQGNNNGYCQDNEISWYDWQAGDRDLLEFTRKLIEL
                     RKQHPVFRRRRWFQGRPIHGAAVTDLGWFTPEGIEMAEEHWGEGFAKAVGIFLNGEAI
                     DDPDPRGERIVDDSFYVLFNAHHEPLRFVLPKEEWGKDWTVVLDTAGTRPEQQERHKA
                     GEEVGVEARSLKVLRRVT"
     gene            3665..6322
                     /gene="treY"
                     /locus_tag="VF182_10945"
     CDS             3665..6322
                     /gene="treY"
                     /locus_tag="VF182_10945"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018174429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX6437638.1"
                     /translation="MSPEFRATYRIQLHRGFAFDDAAAITDYLAELGISHVYCSPYLQ
                     AAHGSTHGYDVVNPHQVNQELGGSTAHARLCAAIAEVGLEQVLDIVPNHMAIGGRENP
                     WWWDVLENGPSSRYAGYFDVDWDPPEAKLRNTVLLPVLGDHYGRVLEQSELVLQREGG
                     QFIIRYHDHVFPVSPRSLDELIATAAQRCDSDDLAFLAEALRQLPPSTATDWDAVQRR
                     HRNKEVLRHMLVRLCDEQPQVAAALDQTVAELNSDPDRLDALLERQNYRLAYWRTAER
                     ELGYRRFFDINSLVGLRAEDERVFADTHALILDWLARGVFDGVRVDHPDGLRDPEQYF
                     DRLHTACPRAWITAEKILEPGETLPESWPIAGTTGYDFIYRVNNLFVDSAAEAALTRF
                     YGEFTGEATDFAALVRDKKQFVLREILASDLNRLTALLVDICERHRRHRDYTRHELHE
                     ALRELIASFPIYRTYVRAEAGVISDEDARIINSTIDDAKARRPDLDAELFDFFRSLLL
                     LRERGPLETEWVMRLQQLTGPVMAKGLEDTAFYCFNRLVSLNEVGGDPGHFGLSPDEF
                     HTACAETQARWPRTMLASSTHDTKRSEDVRARISLLAEIPESWTDAVTRWAELNDRYR
                     REGFPDRNTEYLLYQTLVGAWPIETDRVLAYMEKASREAKTYTSWTDPNSIYDDALRA
                     FVEGTMNNSEFTENLQAFVKPLIEPGRINSLAQTLIKLTAPGIPDFYQGTELWDLSLV
                     DPDNRRPVDYQLRRRLLAEMKCLSAEQLWTRADQGLPKIWLIRQTLHVRHERQLFQPQ
                     DSYRSLMPSGAKREHVIAFARNERAITVVPRFPLKLAGEWGDTAIEIPDGRWRNYFTG
                     EKPDGKLVSVAELLTRFPVALLLREDENR"
     gene            6319..8079
                     /gene="treZ"
                     /locus_tag="VF182_10950"
     CDS             6319..8079
                     /gene="treZ"
                     /locus_tag="VF182_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017940807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX6437639.1"
                     /translation="MTPFRVWAPCAQRVQVDVAGNRHPMTATDYGWWHAELALDDNRA
                     DYAFVLDGGELLPDPRSPWQPRGIHGASRLVDHKAFSWTDQRWQAGPVSGAIIYELHV
                     GTFTPQGTFAGVIDKLDYLVELGVTHIELMPVAEFSGSRGWGYDGADVYAPHHAYGGP
                     DELKRLVDACHGRGLAMILDVVYNHLGPAGNYLARFGPYFTERYATPWGQAINFDGPD
                     SDEVRRFICDNALMWLRDYHFDGLRLDAVHALIDTSATHLLEQLACEVAELQAQSGRH
                     LILIAESDLNDPRIVRSQEIGGYGIHAQWSDDFHHALHTVLTGERDGYYADFGSLADL
                     AKAIERVFVYDGSYSAFRRRRHGRAPKVIFGHSFLGYLQNHDQIGNRAKGERSSHLLS
                     VGRLKIAAALVLSAPFIPMLFQGEEWAASSPFLYFTNHQDAELGRLVTEGRRREFAAF
                     SAHSDDVPDPQALETFERSKLVWDECGRKPHSEMLDWHRRLIRLRHEFAALSDARLDR
                     VSVTFDEQQKWLVMARGPIAVACNLSQRRQHTPLSSGQDARILLASEKEIRIVGDGIE
                     LPPDSVAVVEASGNHSAVRR"
     gene            8081..9910
                     /gene="malQ"
                     /locus_tag="VF182_10955"
     CDS             8081..9910
                     /gene="malQ"
                     /locus_tag="VF182_10955"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX6437640.1"
                     /translation="MASVDNSAAIVQRYQDAFGNWREASDETCAAILATMEQTPEEAD
                     ASVWVVRRGERKHLPGPAEITLEEGAELRAETDLPEDLPLGYHTLRYLDREDETKLIV
                     SPGLCYLPEHLRIWGWSAQLYALRSAGSWGLGDFADLRELGRWSSQELDAGLLLINPL
                     GAASPYAQQERSPYFPSSRRFRNPLYLRIEEIPGAAEVQLDLERLAAAGKALNLQPRI
                     DRDAVFRLKKDALEILWQRFSGDPRFDKFCREQGEPLQQFAAFCVLAEHYQTGWRQWP
                     AEYRTPRSPAVIHFARERSTRVRYFQWLQWLLDEQLAKAAAEISLMQDLPIGVDSNGA
                     DAWAWQEVFAEGFSVGSPPDEYNAQGQDWGLPPFIPSKLRTAGYDPFIQTLRGALRHA
                     GGLRIDHVMGLFRLFWIPQGASPTAGAYVRYPQDELLAILALESQRSKAYIVGEDLGT
                     VEEGVRTKLADSRVLSYRLLWFESDPPARYPELSLAAVTTHDLPTVAGLWSGADLKAQ
                     RDLGLEPNEEGTLKISERLSAMTGSTDETPVEEVITRTYELLAQTPSAIVTATLEDAL
                     AVEERPNIPGTTETSWSLALPESLEALKASPLARAIGERLRGR"
     gene            10183..12567
                     /locus_tag="VF182_10960"
     CDS             10183..12567
                     /locus_tag="VF182_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6437641.1"
                     /translation="MEHSHTVRGYCALCTAHCATIATVENGKVIRLDPDHDHPNGGVL
                     CIKGKAAPELVYHPDRLNYPLKRTRPKSDADPGWRRVSWDQALEEIAERLIFIRERFG
                     ARAVALAKGTRGGTSVSDAERWLARFLNSFGSPNWVSTTHVCNWHKDTGFSYTFGVEI
                     PTPDVAQSNAFLLWGHNPSSTSLILAHDIVAAKKRGMKIVAVDPRRVGTAAQADVLLQ
                     VRPGADGALALALIDVVIEEKLYDQEFVSQWTNGPLLVRSDTGAILTEADLMIGGSAH
                     RFIVWDESGDAAVVYDPSSGSFDKPSVRPALTGERRIKTKSGEEIFCQPVFAALAALA
                     ANYNAARSRSITWVDSEKVRQAAHLLAANRPVSLYMHNGVGQHTNATQTSRAIATLYA
                     LLGDFDRPGGNVVFPKSGVNAVAGKEFLPNEVALQRIGRERKPLGPPAKPGNCAAYDI
                     FTAILEGTPYQVKAVLNFGSNTIMSTGDSQRACEAFRALDFAVATELFMTPTAELCDY
                     VLPATSFLEMGNLAAGFEHRPKGKLHLQYRPAAVPPLAERRSDTWMIFELAKRVGLGR
                     EFWEGDAEAGYEHELEPTGVTLAQLKGSPGGISVAATPRYRKFAATDEHGIVRGFNTA
                     DKKVAIYSHTFADHGFSPLPEYVEPLVSPVSRRDLAAEYPLVLTNAKFTTYIHSQLRG
                     LSSLRKASPEPTADIHPDTAAPHGIKNRDWMIIESPRGAITARARVTEAMAPGVVCCQ
                     HGWWQDCKQLDLPGYVSVGSGSANPSLLIGSDLADPISGSLPHRSYLCRVRPAT"
     gene            complement(12845..14026)
                     /gene="chrA"
                     /locus_tag="VF182_10965"
     CDS             complement(12845..14026)
                     /gene="chrA"
                     /locus_tag="VF182_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX6437642.1"
                     /translation="MGDKIGLWPLVAYFLKLGTIGFGGPVALVGFMHRDLVEQRRWIT
                     EDTYKLSLALAQIMPGPLAAQTAIAIGYFEGGVSGATMVGISFVLPSFLMVIGISLVY
                     VAYGGLWWMQALFYAIGATVIAIIAIAAYKLARSTNKRDPMLWSIFGVLTVMTAWAEA
                     ELAEFFIVAGLIVLLVYAWPGRKRAVLMALGATALGLAIWAMEAWLRQAQTVGHSSDI
                     LAQILLFFTKAGAFVFGSGLAIVPFLQQGVVQQFGWLNQHQFLDAVAVAMITPGPVVI
                     TVAFIGFLVAGLAGAIMASIGIFLPVYIFTIVPAPWFKRHRDNPQLKAFVNGATAAAT
                     GAITGAVIVLGARAITDLPTAIIALVSLVVLWRFKISEPVIVTISGIVGLILWPLLKI
                     G"
     gene            14410..14715
                     /locus_tag="VF182_10970"
     CDS             14410..14715
                     /locus_tag="VF182_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437643.1"
                     /translation="MLRRRLALLLVVSWVILSGFDVTEDLDLPERIALHQTTDALSLG
                     AGSAGLLAGNIVESADHPHLPCASRSAERYLEFTASYVPSFSQRAFKLHKVNQVFLI"
     gene            complement(15296..>15746)
                     /locus_tag="VF182_10975"
     CDS             complement(15296..>15746)
                     /locus_tag="VF182_10975"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEX6437644.1"
                     /translation="VPRTLTALQVLDHIKRSGSHLVLVIDEYGGIEGLLTHHDVLEAI
                     AGDMPFGETVMPPKAVQRADGSWLVDGMIPVDEFKEIFDLDSLPGEKHDAYQTLGGFI
                     FTRMGRVPSVSESFEWNHLRFEIVDMDGKRIDKVLVSHVTEKGAAEL"
BASE COUNT         3420 a         4384 c         4431 g         3511 t
ORIGIN      
        1 ttgagccttc cgccgccgta ctcctcgacc atcagccgag tcagcattga gtgtatatcg
       61 ttcgacactc cgcccaacgt gcgcgacaat tggctggcct cgaccaggcc gtcgagagaa
      121 gcgatggcga gcaggcgacg ataaccgacc cgtccgacat gatgccgaaa gtaaagaccg
      181 gaagccgacg ctggggggga tacgtcgagg gcggcgcggc gcagcagccc gtcgcgaacc
      241 ttctcacgcc tgagggccgc aagattcaac attgagagtt cccatgcttg ccaagcccgt
      301 tcgatccggt cccgcagctg acatagataa tctgaacgtt cattgtcgta gtttctgaga
      361 tcgtcgaacc agaaaagttt cagacgattg atttgataaa gcacgcgctg caagaacagc
      421 cgggcgcagt tgtcggcttg gcttgaatga aagttagcgg cgtcagtagc ggtcgggtac
      481 gcagagttca gcgctttctg aagggcgcat tcaaatagcc ctacagccga cgggtggttt
      541 ctgacgtagt cgtccaaaca atcgaccttt ctcagttcgg caaaactctc ttccgcttca
      601 tcgtactctg ccgaagaact tcgcttctcc gtttcggcat acctcgactg tgttcggcta
      661 ctcttttcag cttcgtcaat gcgcattcat gctccttgtt ctaagtattt gctttgcgcc
      721 agaaagcccc accccggagc ctgcgcagtg cgcatcgtta tgcgcagcgg atccgtcgtt
      781 cgaggggccc aaataataca agttgttccg tctgggcgac gctagaagcc cgccaccgat
      841 aaagcccagt gggcgcttta cgagcgtgtg acgcagaggt tcgctgcggg caagcagctc
      901 aacggctggt cttcaaacat aattgtcgga cagtaaaggg ttctgccacg tccttgctcc
      961 accagcgcgc atctgacaag agctcaggac gtgccagcgg ctacgacccg tgatagacgc
     1021 attgtgcaag aatcacgccc agtatcttga tcaatcagag cgcctcaaga agtcaatcgt
     1081 tctaacgatc gggcctcaat caaattgaac tcagacagca gcggtgatgc gccagccgag
     1141 gggctgaaag gctgcgatcc agagttctcg gcgcctcgct cgcggaaaat gttcagtgta
     1201 gcgtcgatgg gatacggtca ccgcaatggt tgttccagat tcgcacagca tccaggcgat
     1261 acttgctgag cttcatccaa ttcgcaaagc tcgatgcgag ttcctcgcgc aggtcgatcg
     1321 cgagatcctg tggcactatc gggatcatgc cggtgagaaa gacgctcgtt ggatgttcct
     1381 aacgagtgaa ccgaacctag acgctctccg cataaaagca gattcgtaag ctgctggcat
     1441 ttcttttgct ctacaatcga acttcagtta tgtaacaagt taaaaaacgc gcagtatcta
     1501 cgattgaatc acatcgagac aagctccagc agctccggct aaatcattcg aagaatcgcg
     1561 tgggagtggt gtatgaaggt atggccgggg aacaattatc cactaggcgc gtcctatgac
     1621 ggcgccggta ctaatttttc gattttctct gaggtagccg agcgagtgga actctgtttg
     1681 ttcgatgaac agggaaacga aacacgttgt gaccttccgg aagtgacgag cttatgctgg
     1741 catggttacc tgcccaacat cgttcccggt cagcgttacg gctttcgggt tcatggaccc
     1801 tgggccccgc aagaaggcat gcgctgtagc ccggcgaaac tattgcttga cccttatgct
     1861 cgcgcgatcg tgggccaggt ggattggaat gaagcaattt tctcctacgc ctttgatgat
     1921 cccgatgggc ctctcaacca agccgacagc gctcctttca tgcccaaatc ggtggtgatc
     1981 aatccatttt ttgattgggg caacgaccgc catccacgaa cccgatggag tgaaacgatc
     2041 atttatgaaa cccacgtcaa aggtttctct aaacggttct cagatatccc ggaagagctg
     2101 cgcggaacct acgccggtct cgggcacccc aaagcgatcg agtatctgac ccagctcggg
     2161 attagcgcag tcgagttgat gccagtccac caattcgttc acgactcaca tcttctcgag
     2221 cgcggcttgc gaaactattg gggctataac tcgatcggtt atttggcgcc gcacaatgaa
     2281 tactcatcgg gcggtcaaac cggccagcag gttcaggaat tcaaacagat ggtgaaggcg
     2341 cttcatgaag cgggcatcga ggtaatcctc gacgtggttt acaaccatac cgccgagggc
     2401 aatcacctgg ggccgacact ttcgtttaaa ggcctcgata acgcagcgta ctaccgcctg
     2461 atgaatgacg atcgccgttt ctacatggat tacaccggca ccggcaatac tctgaacatg
     2521 cgtcatccgc acgtgctgca gctgatcatg gattcgctgc gctattgggt gctcgaaatg
     2581 cacgttgacg ggtttcggtt cgatcttgcg tccaccttgg cgcggggtct acacgacgtc
     2641 gaccggcttt cggctttctt cgacatcatt caacaggatc cagtcatcag ccagaccaag
     2701 cttattgctg aaccctggga tgtcggcgaa ggcggttacc aggtggggaa cttcccgcca
     2761 ttgtggctcg aatggaacgg caagtaccgc gattgcgtgc gtgattattg gcgcgggcag
     2821 gaccagacgt tgggtgagtt tgccttccgc atcaccggca gctccgatct ctacgaggga
     2881 acgggccggc ggccgtatgc aagcgtgaac ttcataaccg cccatgacgg atttacgcta
     2941 aacgatctgg tttcatataa cgataagcat aacgaggcca atggcgaaga aaaccgcgac
     3001 ggcgagagcc acaatcgttc ttggaactgc ggcgccgagg gacccacaga cgatccggcc
     3061 gtcaacgcgt tgcgcaacca gcaaaaacgc aactttctat cgagcttgtt cctctcggaa
     3121 ggcattccga tgcttctcgg cggcgacgag atcggccgca cccaacaggg caacaacaat
     3181 ggctattgtc aggacaacga gatctcttgg tatgattggc aggcgggcga tcgagatttg
     3241 cttgaattca ccaggaagtt aatcgagttg cgcaaacagc atccggtgtt tcgcagacgt
     3301 cggtggtttc agggccgtcc gattcacggc gctgcggtca ccgacctggg ctggtttacg
     3361 ccggagggaa tcgagatggc cgaggaacac tggggcgaag gctttgccaa agcagtcggt
     3421 atttttctca acggtgaagc tatcgatgat ccggatccgc gcggcgagcg aatcgtcgat
     3481 gacagcttct acgtgctttt caacgctcac cacgaaccgc tgcgctttgt tctcccgaaa
     3541 gaagagtggg gaaaggattg gacggtcgtt ttggacactg ccggcacacg gccagaacaa
     3601 caggagcggc ataaggcggg cgaagaagtt ggcgtggagg cgcgatcact gaaggtgctg
     3661 cgtcgtgtca cctgagtttc gggctacgta ccgcattcag ttacatcgcg gcttcgcctt
     3721 cgacgatgcc gcagcgatta ccgactacct ggctgaactc ggcatcagtc atgtttattg
     3781 ctcgccatac ctgcaggcag ctcacggtag cacgcacggt tatgacgtgg ttaacccgca
     3841 tcaggtcaac caggagctcg gcggaagcac cgctcatgct cgcttatgcg cggcgatcgc
     3901 ggaggtcggc ctcgagcagg tgctcgatat cgttcccaac cacatggcta tcggcggtag
     3961 agaaaaccct tggtggtggg atgttctcga gaacggacct tcaagccgct atgcgggata
     4021 tttcgatgtc gactgggatc cgcccgaagc caaactgcgc aacacggttc ttcttccggt
     4081 tctgggcgat cattacggac gagttcttga acaatccgaa ctcgtactgc aacgagaggg
     4141 cgggcagttt atcatccgtt atcacgatca cgtcttcccc gtttcaccta ggtctttgga
     4201 tgagctcatc gccactgccg ctcaacggtg cgattcagac gacctggctt tcctcgcgga
     4261 ggcattgcgt caactgccgc cgtcaacggc gactgattgg gacgcagtgc agagacgtca
     4321 tcgcaacaaa gaggttctgc gacacatgct ggtgcgcctg tgtgatgagc aaccgcaagt
     4381 cgcggccgct ctcgatcaaa cggtcgctga gcttaattcc gatcccgatc ggttggatgc
     4441 gttactggag cgccaaaact atcgcctggc atactggcgt accgcggaac gagaactcgg
     4501 ttaccgccgg tttttcgata tcaacagttt ggttggcttg cgggcggagg acgagcgggt
     4561 gtttgccgat acccacgcct tgatcctcga ctggttggcc cgaggcgtgt tcgatggcgt
     4621 gcgcgtcgat catcctgacg ggctgcgcga tcctgaacag tacttcgatc ggcttcatac
     4681 agcctgtccg cgcgcgtgga tcacggccga gaaaatcctc gagcccggcg aaacgcttcc
     4741 tgaatcatgg ccaatagccg gtaccacggg ctatgacttc atctatcgcg tgaacaatct
     4801 tttcgtcgac tctgctgcag aggccgcgct gacccgattt tacggcgagt tcaccggcga
     4861 ggcgaccgac tttgccgcgt tggtgcgtga caaaaaacaa ttcgtcttgc gcgaaattct
     4921 tgccagcgat ctcaatcgtc tgaccgcctt gttggtcgat atttgcgagc gacatcgccg
     4981 gcatcgcgac tacacgcgac acgagctgca tgaggcgcta cgcgaattga tcgcgtcttt
     5041 cccgatctat cgaacttacg tccgcgccga agccggagtt ataagcgacg aggatgctcg
     5101 aatcatcaat agcaccatcg atgacgccaa agcgcgccgg ccggacttgg acgctgagtt
     5161 gttcgatttt tttcgcagcc ttcttctgct gcgcgaacgc ggtccactcg agaccgagtg
     5221 ggtcatgcgt ttgcagcaac tgaccgggcc ggtgatggcc aaaggtctcg aagacacagc
     5281 tttttattgt ttcaaccggc tggtttcgct aaacgaagtc ggcggcgatc cggggcactt
     5341 tggcctttct ccggatgaat tccatacagc ctgcgccgag acccaggcgc gctggccgcg
     5401 caccatgctc gcttcctcga cacacgacac caagcgcagc gaagacgtgc gcgcgcgcat
     5461 cagtttgctt gcagaaattc cagagagttg gaccgacgcg gtgacaagat gggccgagct
     5521 caatgaccgc tatcgtcgtg agggttttcc cgatcgaaat actgaatatc tgctttatca
     5581 aacgctggtg ggcgcctggc ccatcgaaac cgatcgcgtt ctcgcgtaca tggagaaagc
     5641 ctcccgcgag gccaagactt atacctcctg gactgatccg aattcaatct acgacgatgc
     5701 tttgcgagct ttcgtcgaag gcaccatgaa caattccgag ttcacggaaa atctgcaagc
     5761 gttcgtaaaa ccgctgattg agccgggccg gattaattct ctggcgcaga cactgattaa
     5821 actcaccgcc cccggtattc ccgattttta ccaaggtact gaactgtggg atctaagtct
     5881 ggtggatccc gacaaccgcc ggccggtgga ctatcaattg aggcgccggt tgctcgccga
     5941 gatgaaatgc ctgtcagccg agcagttatg gactcgggca gaccagggtc tgcctaaaat
     6001 ctggttgatt cggcaaacac tccatgtgcg acacgagcgg cagttatttc agccgcagga
     6061 tagttaccgt agcttgatgc catccggagc aaagcgcgaa cacgtcatcg cgttcgcgcg
     6121 caatgagcgc gcgatcacgg tggtgccgcg ctttccttta aagctcgccg gagaatgggg
     6181 cgataccgcc atcgaaatcc ccgatggccg ttggcgtaat tacttcaccg gcgaaaagcc
     6241 ggatggcaaa ctagtttcag tggctgagct gctcacgcgt tttccggtag cgcttttatt
     6301 acgagaggat gaaaatcgat gactccgttc cgagtttggg cgccttgtgc ccaacgggtt
     6361 caagttgatg ttgccggcaa ccggcacccg atgacagcca ccgattacgg gtggtggcat
     6421 gccgagctag cgctggatga caatcgcgct gactacgcct tcgtgctcga tggcggtgag
     6481 ctgctccctg atcctcgctc cccttggcag ccgcgcggca ttcacggcgc ctcccgcctg
     6541 gtcgatcaca aagcattttc atggacggat cagcgctggc aagccggccc cgtttctggg
     6601 gcgattatat acgagcttca tgtcggtact ttcacgccgc aaggaacttt cgccggagtc
     6661 atcgacaagc tcgattatct agtcgagctc ggcgtcaccc atatcgagtt gatgccggtt
     6721 gccgaatttt cggggagcag gggttggggc tatgacggcg cggacgtgta tgcgccgcat
     6781 catgcgtacg gcgggccgga tgagttgaag cgtctggtgg atgcctgtca tgggcgcggc
     6841 ctggcgatga ttctcgatgt cgtttataac cacttgggac ctgcggggaa ttatctggcg
     6901 cgcttcgggc catatttcac cgagcgttat gctacgccgt ggggccaagc gatcaatttc
     6961 gacggtccgg atagcgacga ggttcgacgt tttatttgcg ataatgcttt gatgtggctg
     7021 cgcgattatc acttcgatgg gctgcggctc gatgcggtgc acgcgctgat cgacacctcg
     7081 gctacgcatc ttcttgagca gttggcgtgc gaggtggccg aactccaggc ccaaagcggc
     7141 cggcatctaa tactgatcgc cgagagcgat ctcaacgatc ctagaatcgt ccgctctcag
     7201 gagatcggcg gttacggcat tcatgcgcaa tggagcgatg atttccatca tgcgttgcat
     7261 actgtgctca ccggcgagcg cgatggttac tatgctgatt tcggctcgct ggctgatctg
     7321 gccaaagcca tcgagcgggt attcgtctat gatggcagtt actcggcatt tcgccgacga
     7381 cgacatggaa gagctcccaa ggttattttc ggccattctt ttctcggcta cctgcagaat
     7441 cacgatcaga ttggcaaccg cgccaaagga gaacggagca gtcatctctt gagtgtcgga
     7501 cggctcaaga tcgctgcagc cttagtgctg agtgcgcctt tcattccgat gttgttccaa
     7561 ggcgaagaat gggccgcgag ctcgccgttt ttgtatttca ccaatcatca agatgccgaa
     7621 ctgggtcgct tggtaaccga aggccggcgc cgggagtttg ccgccttcag cgcgcactcc
     7681 gatgatgtgc ctgatccgca agcgcttgag accttcgaac gttccaagct cgtttgggac
     7741 gaatgcggcc gcaagccgca ctctgaaatg ctcgattggc accgccgact gatccggctg
     7801 cgccacgaat ttgccgctct ctcggatgct cggctggacc gtgtcagcgt cacttttgac
     7861 gagcaacaga agtggttggt gatggcgcgt ggtccgatcg ccgtagcttg taatctgagc
     7921 caacgccggc agcacacgcc gttaagcagc ggccaagatg cgcgcatcct gcttgcttcg
     7981 gagaaagaaa ttcgaatcgt cggcgatgga atcgaattgc cgccggactc ggtggcggtc
     8041 gtcgaggcaa gcggtaatca ttctgcggtg agacgataac atggctagcg tggacaactc
     8101 agcagccatt gtccaacggt accaggatgc tttcggcaac tggcgtgaag ccagtgatga
     8161 gacctgcgcg gcgatcctcg cgacgatgga gcaaacgccg gaagaagcgg acgcctcggt
     8221 ctgggtagtt cggcgcggcg agcgaaagca tctgcccggt ccggcggaaa tcacgctcga
     8281 agagggcgct gagctgcgcg ccgaaactga cctgccggaa gatctgccgc tgggttatca
     8341 cactttgcgc tatctcgatc gtgaggatga aactaaatta atcgtcagcc cgggactctg
     8401 ttatctgccc gagcatttgc ggatttgggg ttggtcggcg cagctctacg cgcttcgctc
     8461 ggccggtagt tggggcctcg gcgatttcgc cgatctgcgt gagctgggcc gctggtcgtc
     8521 acaagagctc gatgccggat tgcttttaat caatccgctg ggcgcagcat cgccttatgc
     8581 gcagcaggag cggagtccct actttcccag cagccgccgt tttcgcaatc cattgtacct
     8641 gcgtatcgag gagatcccgg gcgcggccga ggttcagctt gacctggaac ggttagccgc
     8701 cgcgggcaaa gctctcaatc ttcagccgcg gattgatcgt gatgcggtat tcaggctcaa
     8761 aaaagatgcg ttggagatac tttggcagcg cttttcaggc gatccgcggt tcgacaagtt
     8821 ttgccgcgag cagggagagc cgctacaaca attcgccgcc ttttgcgtgc tcgctgagca
     8881 ctatcagacg ggttggcgtc agtggcccgc tgagtatcgc acgccccgat cacccgcagt
     8941 gatacacttt gccagggagc ggtctacccg ggtgcgctat tttcaatggc ttcaatggct
     9001 cctcgatgag cagttggcaa aagccgctgc cgaaatatct ttgatgcaag atctgcccat
     9061 cggagttgat tctaatggcg ccgatgcctg ggcatggcaa gaggtgtttg cggagggttt
     9121 tagcgtcggc agcccgcccg acgaatacaa tgcgcaaggt caggattggg gattgccgcc
     9181 gtttattcca tcaaagcttc gcactgccgg ctatgatccg ttcattcaga cgctccgggg
     9241 agcactgcgg catgccggag ggcttcgcat tgatcatgtc atgggactgt tccgtttgtt
     9301 ctggatcccg caaggcgcat ctccgactgc cggcgcttac gttcgatatc ctcaggacga
     9361 gctcttggcg atactcgctt tggaaagcca gcggtcgaaa gcgtatatcg tcggcgaaga
     9421 tctcgggacc gtcgaagaag gcgtccgcac aaagctcgcc gacagtcgcg tcctgtctta
     9481 ccggctgctc tggttcgaga gcgatccgcc cgcccgttac cctgaactgt cgctcgcggc
     9541 ggtgaccacc cacgacttgc cgacggttgc aggtctgtgg agcggcgctg atttgaaggc
     9601 tcagcgtgac ttaggtcttg agcccaacga agaaggaacc ttgaaaattt ccgaacgcct
     9661 gagcgcgatg acgggtagca ccgacgaaac ccccgtggaa gaagtgatca cgcgaaccta
     9721 tgagcttctc gcgcaaacgc cttcggctat cgtcaccgcg acattggaag acgctttggc
     9781 ggtggaagag cggccgaata tcccgggcac cacagagaca tcgtggtctc tcgcgctgcc
     9841 tgagtctctg gaagctctca aggccagtcc tctcgcccgc gccatcggtg agcgactgcg
     9901 cggtcgttga cgcccatgca gcgaatcgac tgcggattga acgtaagttt tgactagtgc
     9961 cgatgaaaac gcctgtatct agccgatcgt gatttggggt gccgcctgca cctttcaagc
    10021 ttttcgccca agcttcccaa tcgcttaccg agttcctgaa ggctttgtcc caaaatctga
    10081 acaaacgatg cgcaacggca agaatcatct cgcaggccga agcttgcgga gagttgcgca
    10141 gccatcggcc tttgacatag tcttaactcc atggctgatc acatggaaca ttcacacacg
    10201 gtgcgcggct attgcgcgct ttgcaccgct cactgcgcca ccatcgcaac agtagaaaac
    10261 ggtaaagtaa tccgcctcga tccggaccat gatcatccga acggcggcgt gctttgtatc
    10321 aaaggcaaag cggcgccgga gttggtttat catccggatc gactgaatta tccgctcaaa
    10381 cgcacgcgcc cgaagagcga tgccgatccg ggctggcgaa gagtcagttg ggatcaggcg
    10441 ctcgaggaaa ttgccgagcg gctgattttt attcgcgaac gcttcggcgc gcgcgcagtg
    10501 gccctagcca aagggacacg cggcggcacc tccgtttccg atgccgagcg ctggttggcg
    10561 cgctttctca atagttttgg gagccccaac tgggtatcga ctacgcatgt ttgtaactgg
    10621 cacaaggata ccggcttcag ctatacgttc ggcgtggaga ttccgacacc cgacgtagct
    10681 caaagcaacg cttttctcct ttggggacac aacccaagct cgacgtctct gattttggct
    10741 catgacatcg ttgcggcaaa aaaacgcggc atgaaaatag ttgcggttga tccacggcgc
    10801 gttggaactg ccgcccaagc cgatgtgctg ctgcaggtta ggcccggcgc agatggggca
    10861 ttggcgttgg ctctgatcga tgtggtcatc gaagaaaaac tctacgatca agagttcgtg
    10921 agtcaatgga ctaacgggcc tcttcttgtg cgaagcgata ccggagcgat tctcactgaa
    10981 gccgatctca tgatcggggg atcagcgcat cgatttattg tctgggacga aagcggcgat
    11041 gctgctgttg tctatgatcc aagctccggc agtttcgaca agccgtcggt gcggcctgca
    11101 ctgacaggtg agcggcgcat taaaaccaaa agcggcgagg aaatcttctg ccagccggtg
    11161 ttcgcagcgc ttgcggcgct ggcagcgaac tataatgccg cgcgctcccg atctattact
    11221 tgggtcgaca gcgagaaggt caggcaggcg gcgcatctgc tggcggccaa tcggccggta
    11281 agtttgtata tgcacaatgg cgtggggcag cacaccaatg cgacccagac cagtcgagcg
    11341 atcgcaaccc tttacgcgct attgggagat tttgaccggc cgggcggcaa cgtagttttc
    11401 cccaagtcag gggtaaacgc ggttgctggt aaggaatttc tgccgaatga agtcgcgctc
    11461 cagagaattg ggcgcgagcg caaaccgctt ggaccgcccg cgaagcccgg caattgcgcc
    11521 gcttacgata tcttcaccgc tattctcgaa ggcactccgt atcaagtcaa ggcagttttg
    11581 aatttcggct ccaataccat catgtctacc ggggattctc agcgggcgtg tgaagccttt
    11641 cgcgctctcg actttgccgt ggccaccgag ctattcatga cgccaacggc ggaactctgc
    11701 gattatgtct tgccggccac cagctttctc gagatgggta atttagccgc cggattcgaa
    11761 caccggccga aaggaaagct tcatttacag tatcgccccg ccgcggttcc gcctctggct
    11821 gagcgacgtt ccgacacctg gatgatcttc gagttagcta aacgcgtggg tcttggccgt
    11881 gagttctggg aaggcgatgc cgaagcgggt tacgagcacg agttggaacc gactggcgta
    11941 acccttgcgc aactcaaggg atctcccgga ggaatcagcg ttgccgctac accgcgctac
    12001 cgaaagttcg ctgcaaccga cgagcacggc attgtccgcg gctttaacac ggcggataag
    12061 aaagtggcga tctactcaca tacatttgcc gatcacggtt tttcgccttt gcccgagtat
    12121 gtggaacctt tggtcagccc ggtcagccgc cgcgatttgg ccgccgaata cccgctggtg
    12181 ctgactaatg cgaaatttac aacttatatt catagccagc tgcgcggtct ctccagtctg
    12241 cgcaaggctt cgcccgagcc gaccgccgat attcatcccg atacggcggc gccgcatgga
    12301 attaaaaatc gcgactggat gatcatcgaa agcccgcgcg gggcgatcac ggcgagggcg
    12361 cgcgtcacgg aagcgatggc gcccggagtg gtctgttgcc agcatggatg gtggcaggac
    12421 tgtaagcaac tcgaccttcc cggctacgtc tcggtcggaa gtggttcggc taacccgagc
    12481 ctgctgatcg gctccgatct tgctgatccg attagcggct cgctaccgca tcgctcttat
    12541 ctctgtcgtg tgcgccccgc aacgtgatcg agccagctgg caatttggtg cgcaagagcg
    12601 aacggagagt tgaatatcgt attattcttt cttcaaagtc tttgtagaag cgggcaaagc
    12661 ggtcgtgaac ttagtcctcc ccccggccgg gtcatacaat tcgtgattct gcctacggga
    12721 gagtatctgt ttagcaattg ctcggcgcaa tccggtgagg tgggcatgcc atgagaatag
    12781 cgctcgccac tctgcaggtt gctcggtagg aaagcggtcg aaaacagatc aagggaagcg
    12841 gtatttagcc gatcttcaac agcggccata gtatcaagcc aactatcccg gagattgtaa
    12901 ctatcaccgg ctctgatatt ttgaagcgcc acaaaacgac caggcttacc agcgcgatta
    12961 tcgctgttgg caagtctgtg atggcacgtg ctccgagcac gatcactgcg cccgtaattg
    13021 cgccggtcgc ggcggccgta gcgccattga cgaaagcctt gagttgggga ttgtctcgat
    13081 gccgcttgaa ccaaggggcc ggaacgattg taaagatgta taccggcagg aagataccaa
    13141 tcgatgccat aatggcccca gctaaaccag ccacaagaaa gccgatgaat gcgacagtga
    13201 taacgactgg ccccggcgtg atcatagcta ccgcgaccgc atcgaggaat tggtgctggt
    13261 tcagccagcc gaattgctga acaacacctt gttgcaggaa cggtacgatt gctagaccgc
    13321 ttccgaacac aaaggcaccc gccttagtaa agaacaaaag aatctgcgcg agaatgtcag
    13381 atgagtgccc caccgtctga gcttggcgta gccaggcttc cattgcccaa atcgcgagcc
    13441 ccaaagctgt agcacctaac gccatcagca cagcccgctt tctgcccggc caagcataca
    13501 caagcagcac gattaatcct gcaacaatga aaaattccgc cagctccgcc tctgcccaag
    13561 cggtcatcac cgtaagaact ccgaaaatgc tccaaagcat cggatcgcgt ttgttcgtac
    13621 tacgtgcgag cttgtaagca gcgatggcaa tgatggcgat gactgtggcg ccgatggcat
    13681 agaagagagc ctgcatccac cacaagccac cataggcgac gtagaccagc gagatgccga
    13741 tcaccattaa aaaagagggg agcacgaagc ttattccgac cattgtcgcg cccgaaacgc
    13801 cgccttcaaa atagccaata gcgatggcgg tctgtgctgc taagggtccg ggcatgatct
    13861 gggcaagcgc taatgagagc ttgtaagtat cttcggtgat ccaacgccgt tgctcgacca
    13921 aatctcggtg cataaagcct actaatgcga ccggtccacc gaaaccgatt gtgcctaatt
    13981 ttaggaagta tgcgacgagt ggccaaaggc cgatcttgtc gcccatgata ggtttaggct
    14041 cgtcttctag atcgactggg aaaaccatca gtctcaatcc tctctgcctt catagcacag
    14101 gttgaatcag ctagcaggtg tattttcccg gaagcaaatg agtactcgac aaaaatgata
    14161 atcgaaacaa gcccgtgatt ttaagccgtc acatggtgcg aatgatcttc tagagcagtt
    14221 tacccggcgc cacttcgata ctgaaaaaaa acctacccgc aagtgacaat cgtcaaacga
    14281 cttctggcgt gagagtttaa gggaatcaat caacacttca aaaactggaa ttggcgcgga
    14341 tgaattgaat cccccactaa aaggaaatat tgctgttgac atcttccgtg ctccgttaga
    14401 ctatccgcta tgcttcgaag acggcttgcg ctattgctcg ttgtttcgtg ggtgattctt
    14461 tccggattcg atgtcaccga agatctggac ctgccggaac ggattgcgct tcaccaaacg
    14521 accgatgctc tttcactcgg tgctggatca gcgggtctcc tcgctggcaa catcgtcgaa
    14581 tctgcagacc acccgcacct gccttgcgcg agtcgatccg ccgagcgcta tctcgagttc
    14641 accgccagct atgtccccag cttctcgcaa agagccttca aacttcacaa agtaaaccaa
    14701 gtgtttttaa tctgagcacg tcatcttctt ttgttctccc gttctaagct cctttaagtt
    14761 cgcccggaga gcgcaatcgg ttgcggctga ttggctcata aacgtttcga gacacaagga
    14821 gaggatgatg gctgattttc ccttatggtt gaaaggtgtc gtctggctgg ttgtaggttc
    14881 tagcgtgttt tatgcggctg ctcaggttct atactcattg ataaattaac tggcgacgca
    14941 ggagtttggc gtattgtctg ccatttgcgc aatgcgtctt tgcgttcgtt ttattttgca
    15001 tcggtaactt gttccgccag agcggtcttc cattgccttt ttcaatcctc atgaacgatt
    15061 ttttgagacg cacaggctca tcttaatgcc gttttgtttg tgcggagcct tggccgctat
    15121 ctggtaggga gacgcttgtc ccggcctccc tataaaacga tcttggcggt agaaaaaatt
    15181 gccaagacag ccttcaacga ttttcccgcc agcgaaattg cgcgccgctc tgagaggccg
    15241 ccttgggacc gctcaagtcg gacatcgttc tctacgagtc gctctgcgcg gcgagttaaa
    15301 gctcagcggc gcctttttcc gtcacatgtg ataccagtac tttgtcgatg cgcttgccgt
    15361 ccatatcgac aatctcgaat cgcaaatgat tccattcaaa ggattccgac accgacggca
    15421 cgcgacccat cctggtaaaa ataaaaccgc ccaaggtctg ataagcgtca tgcttttctc
    15481 ccggcaagct gtcgagatca aaaatttctt tgaactcgtc caccggaatc atgccatcga
    15541 ctagccatga gccgtctgcg cgttgcacag ccttgggcgg catgacggtt tcgccaaacg
    15601 gcatatcgcc ggcaatggct tccagaacgt cgtgatgcgt cagtaatccc tcgatgccgc
    15661 catactcgtc gatgaccaag accagatggc tgccggagcg cttgatatga tcgagtacct
    15721 gcaatgcagt taatgtccgc ggcacc
//
LOCUS       DASUVS010000197         7399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_736790, whole genome shotgun sequence.
ACCESSION   DASUVS010000197 DASUVS010000000
VERSION     DASUVS010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7399
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736790"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(507..1370)
                     /locus_tag="VF182_10980"
     CDS             complement(507..1370)
                     /locus_tag="VF182_10980"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6437645.1"
                     /translation="MDTAERTKPKDKFVNVDGLQLRYIEEGSGPSVLFLHGASLGSSA
                     DVFLRNLGPLAQAGFRAVAFDLPGFGLSDVPAKQSTAQQRNAIPKFIDAAGLGKTALI
                     AHSRSGGFAVQLALEDPNRYSHVIILGTGSLLPPQTQEQVGKYEAVQARVDKEMAQKE
                     PTLEDARKLLQADTFNHSLISDEDVAIRHSRMTGRNFKAHQERQNQDAPAGGGASQAK
                     PIWQRLTELKMPLLMFFGREDRAHAGERAELLKKMNPEINLHIVNGCKHMVHWDAFDD
                     LMRLGVPFLKS"
     gene            1967..4294
                     /locus_tag="VF182_10985"
     CDS             1967..4294
                     /locus_tag="VF182_10985"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX6437646.1"
                     /translation="MTIKTQTLLMVALLLVIAVVATAGVMVWGSRRGLLAEGETQGLV
                     IARLLARSAAFGARVTSDVEKAIGEQMVVEATIAAHLVALGEMAGVGPEEINRRLKQI
                     ADDTVLDEIWITDEKGHAYLRNITEVDFTFSPDRERQPQAHAFYPLLTGKSKAVVQEA
                     RQREIDTQVFKYVGVAGVDKPRIVQVGYHAGMLRQLQQRMGLTRLVNQLVSEGSVIAI
                     RVLDKNMITVEYAESFRDKKLPEPTETDLADWRRLVSEGRNHSSLEASLLNVLAPIGE
                     EGGELKGGAILVTLPTDHVQAAIQNRIRIATVVSPLVLLLGSVIAIFWAKKISSPIVE
                     LTEMTRRIAGGDFTQRIAISARNEIGTLAASFNEMTRRLNQSIEHLKETTAAKERIES
                     ELQIAHEIQMSMVPKIFPPFPERSEFDLFATLVPAKEVGGDLYDFFFIDDDHLCFAVG
                     DVSGKGVPASLFMAVTKTLFRATAGNGGSPGEILARLNAEICRDNESCMFVTFFCGIL
                     NIHTGQVHYSNAGHNLPFYLSRDGVRPLENFGGISLGLLEQGPYASGGMILRPGESLL
                     LYTDGVTEAMNPSRTLYSDERLEQFLGTTDSSAPRRIIDGLISDVRHFAGQAPQSDDI
                     TVLALQYFGTAKEMLEPVEIRVNNKLSELASANQALTEFGRQYGLADNVLHDLNLALE
                     EILTNIISYGYTDSGEHEIAVRFSIKAGEMRVDVEDDGQPFNPLEAPEVDTSKPLEER
                     TIGGLGVHLVRKLMDGLEYQRREGKNLLVMRKFLL"
     gene            4374..4688
                     /locus_tag="VF182_10990"
     CDS             4374..4688
                     /locus_tag="VF182_10990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6437647.1"
                     /translation="MKELEITEQQSQSTTVLSVVGAVDSNTAPELQQALLRATETSTG
                     GVELDLAKVSYLSSAGLRALLMAAKALQKRSERLKLINVPLPIKNVLNLTGFATFIDV
                     SP"
     gene            4731..6461
                     /locus_tag="VF182_10995"
     CDS             4731..6461
                     /locus_tag="VF182_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6437648.1"
                     /translation="MKIEANVWQRVSGTPSVDIFPIIPKANCVSSNCYIFSAPEALVV
                     VDPGASAEQTRQISRILTDALMVSQRPVLLFLTHCHQDHSQEADLELPTGTEVTRFAH
                     KSGVEALKHRDRNLTTAYLYPWNPEICAAPFDGILFASSSASEPATLESSGGRRFELY
                     TEPATLPDGATLQRQWLSLGAGNRLEIYHTPGHSACSISLRIGSLLMLGDLPFAANPG
                     VCGLDGWNHADLLQTLRNVDWLLEIPDITACCPGHGYCVPAQSMREKLRLMRTEASNL
                     TDVPLMGAERISGLKIYADELLEETTALLTIFSGRLYTVSYCLSMLEEEDSAASRILE
                     SLDIDQIDRILSDFRRVAEAFNSSAMPEWTVVLKGVQVARSLQKVLSAENVRQLLDFS
                     LVDRAQRRLDDYLSLVRGLQFLQTEQPAAVNEIVTELLKRTKETTMTQSADLMEAVDD
                     DQSFLQALTRRLAAYSPLRDIQFEFAPAAQETNANIGAERLDDILTNVMEGMAGMGVK
                     HISIATQVASGQVEIRLSSPDPMDPAAFGKRRLDLYNRTLEWLGGGFECIQHRGNADF
                     IIRLPALKSM"
     gene            6749..7384
                     /locus_tag="VF182_11000"
     CDS             6749..7384
                     /locus_tag="VF182_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437649.1"
                     /translation="MLLVLIGREWIKMTDKSGVPRLENPEDFVRLEILAGLEQKTVIV
                     PVLLADAEMPSAQALPAPLQPFARRNAIEISDSRFHSDVDRLIEAIEKIFNPQRAAVP
                     PIRPEQRNQSSTPFSSASLRPNTGFIAAAIAAVALIVAGIWWFTTGAEQETSFQAEPY
                     IQSPAPKSAENSPRQDTRQSPEQQSPRQSPVQQDPSQTSPAYNPRQSAPRN"
BASE COUNT         1691 a         2006 c         2106 g         1596 t
ORIGIN      
        1 gacctcctca caaacttcgg atttgagagt gatggatcaa tcttctcgtg tggcgacgaa
       61 gatgcacagg aaaatgccga ctgcgaatag tgcatggcaa ttccgatgag ccggacaaag
      121 agcgagcgaa gctgacccgc cgcagcaaac ggtcggtaag agcttgcccc agttcttggg
      181 attgccgatt tccgaacggg cgatagcgtc aacagcgatc aaagcaggcg acgagtccta
      241 aacctaaaca ccaactccgc gcgagcctaa gtctttcgag gttcgctaat tcattcagct
      301 gttcgtgctg ctccgaatgc cattgccaac cggctctaat gttaatcttc tgctagatta
      361 cttatggacg gccttattga tatgcaggct gctcaaaaac ctcagaggca agacgcgcga
      421 agttcgatga gctgaggcgt acgcttcagt acgttgaagc gagtcgggca agcgcaacga
      481 agcatatggg cttttttcag tagccgctag ctcttcaaaa acggcacgcc caggcgcatc
      541 agatcgtcga aggcatccca atgcaccatg tgcttgcagc cattgacgat gtggagattg
      601 atctcggggt tcattttttt gagcagttcg gcgcgttcac cggcgtgcgc gcggtcttcg
      661 cggccaaaga acatcaaaag cggcatcttc agttcagtca gcctttgcca tattggcttg
      721 gcctgcgacg cgccgcctcc cgcgggggca tcctggttct gccgttcctg gtgcgctttg
      781 aaattgcggc cggtcatgcg gctgtggcgg atcgccacgt cttcatcgga gatcagtgaa
      841 tgattgaagg tgtcggcctg caaaagtttg cgcgcatcct ccaacgtcgg ttccttttgc
      901 gccatttcct tgtcgacccg cgcctgtacc gcttcgtact tgccgacctg ctcctgggtc
      961 tgcggcggca gcagtgagcc ggtgccgaga atgatcacat gcgagtagcg gttgggatct
     1021 tccaatgcca gctgaaccgc gaagccgccc gagcgtgaat gcgcgatcaa cgcggtcttg
     1081 cccaatcccg cggcatcgat gaatttcggg atggcattgc gttgctgcgc cgtgctctgc
     1141 ttggcgggca cgtcggagag gccaaaaccg gggaggtcaa aggcgaccgc gcgaaatccg
     1201 gcttgggcga gaggaccgag attgcgcaga aacacgtccg ccgagcttcc cagcgaagcg
     1261 ccgtggagaa acaaaaccga cggtcctgat ccttcttcga tataacggag ctgcagccca
     1321 tcgacgttaa cgaacttatc tttcggtttg gttctttccg cggtatccat aagattgtct
     1381 cctattaagc caaaaaagat caggctgttg gaaaaggcac ttcgatgcgc tgggtcgcct
     1441 caggtcaatt cgtattcttg ttagcgtgtt ccgtgctcgt ggatttcacg gtcacgttca
     1501 tagacacggt ctcggttcat gtgcggcgcg catcaagagc tctttgaaca gcttggatcc
     1561 gagtttttac atagtctgta tctgcgaaag catgacgaac cttttctgag ccgacctata
     1621 acgctgaagc cacgaggtgt caatcacgcg cgagcttagt ggaatgagta aacgagatgc
     1681 tgctggtctg tccgagaaat ccccagggag tatcccggct cacagccgac gtatccgcga
     1741 aagcggctcc ggatttcttt cgattggacg gttccgagtc gcgccacttt tcgcacttgc
     1801 ctgaaatgac gattatttgc tagcgcttgt cctgaatagc gcagcgacta tagacgggat
     1861 tgaacgtttt tgcaccgggt gccatcgcac agtgaaaatc ggggtatttt tttgattcgc
     1921 gatgtggttt gagtgagccg gaagtaatag ggcgaaggag aaacttgtga caatcaagac
     1981 gcaaacgctt ttgatggtag ccctgctgct tgtcatcgcg gttgtcgcta ctgcgggagt
     2041 catggtctgg ggttcgcggc ggggattgct cgctgaaggc gaaactcagg gtttggtcat
     2101 cgcccggctc ttggctcgca gcgcggcctt cggcgcgcga gtcacaagcg atgtcgaaaa
     2161 agcgattggc gagcagatgg tcgtagaggc gacgattgcg gcgcatttgg tggctctggg
     2221 cgagatggcg ggagtcggtc cggaagagat caacagacgc ttgaaacaga ttgccgacga
     2281 tacggtgctc gacgaaatct ggatcaccga tgaaaaaggg catgcttact taagaaatat
     2341 tactgaggtg gacttcacgt tcagtccgga tcgggagcgg caacctcaag ctcacgcgtt
     2401 ctatcctctg cttaccggaa aaagcaaagc tgttgtccaa gaggcgcgcc aacgtgaaat
     2461 cgacacgcag gttttcaagt acgtgggagt tgccggggtg gataaaccgc gcatcgtcca
     2521 ggttggttat catgccggca tgcttcgaca gctgcagcag cgcatggggc tgacccgact
     2581 ggtgaaccag ctggtctccg agggaagcgt catcgcgatc cgtgtcctcg acaagaacat
     2641 gatcaccgtg gagtatgcgg agagcttcag agacaaaaag ctgccagagc cgacggagac
     2701 cgacttggcc gattggcgca gactggtgag cgaaggccga aaccacagct ctctggaagc
     2761 atctttgctc aacgtcctcg ctccgatcgg cgaagaggga ggagagctaa aaggtggagc
     2821 aattctcgtc acactgccca cggatcacgt gcaggctgcc attcagaacc ggattcggat
     2881 cgccacggtg gtgagcccgt tggtattgct gctcggttcg gtgatcgcca ttttttgggc
     2941 gaagaaaatt tccagcccga ttgtggagtt gaccgagatg acccggcgga tcgccggcgg
     3001 ggacttcacc caaagaatcg ccatctcggc acggaacgaa atcggcacac tcgcggcctc
     3061 gttcaacgag atgacgcgtc gcttgaacca atccatcgag catctgaagg aaaccactgc
     3121 ggcgaaggag cgcatcgaaa gcgagctaca gatcgcccac gaaatccaga tgagcatggt
     3181 gccaaaaatc ttccctccgt ttcccgagcg cagcgagttc gatctttttg ccactctggt
     3241 gccggccaag gaggtaggag gggatctcta cgatttcttc ttcatcgatg acgatcacct
     3301 atgttttgcc gtcggcgatg tctcgggtaa gggagtaccg gcgtcgctgt ttatggccgt
     3361 caccaaaacc ctctttcggg cgaccgccgg caacggcggc agtccgggtg agatcctggc
     3421 acggttgaat gccgagatct gtcgcgataa cgagtcctgt atgttcgtga ccttcttttg
     3481 tgggatcttg aatatccaca ccggccaggt ccattatagc aacgccgggc acaatctgcc
     3541 cttttacctg agccgcgacg gcgtacgtcc tttggaaaac ttcggcggaa tatctctggg
     3601 gcttttggag caaggcccct atgcatcagg cgggatgatt ctccgtcccg gcgaaagttt
     3661 actgctttac accgacgggg taaccgaagc catgaatccg agccggacgc tttattccga
     3721 tgagcggctc gagcagtttc taggaacaac tgacagttcg gctccacgcc gaatcatcga
     3781 cggtttgatc agcgacgtaa ggcattttgc tggccaagcg ccgcaatcgg atgatattac
     3841 ggttcttgcg ctgcagtatt ttgggaccgc gaaagaaatg ctcgagcctg tcgagatcag
     3901 ggtcaacaac aaactttcgg agctcgcctc agcgaaccaa gccttgacgg aattcggccg
     3961 gcaatacggt ctagccgaca atgtcctgca cgatcttaat ctcgccttgg aagaaattct
     4021 cacaaacatc atctcctatg gttacaccga cagcggggaa cacgagattg cagtaaggtt
     4081 tagtatcaaa gctggagaga tgagagttga cgtcgaagat gacggccaac cgttcaatcc
     4141 attggaagca ccggaggttg acacgtcaaa gcctctggag gaacggacta tcggtggact
     4201 gggcgtccat ctcgtgcgca agctcatgga cggcttggaa tatcaaagac gtgaaggaaa
     4261 gaatctattg gtcatgagaa agttcctgct ctagcagatc gctttaacac tccctagtgg
     4321 atccatagcc gaagctaggt ggagaatttt tcgatcatta agagaggcgc gatatgaaag
     4381 aactggaaat caccgagcag cagtcgcaat cgacgactgt tctatccgtc gtgggcgccg
     4441 tggatagtaa cacggctccg gagctgcagc aggccttgtt gcgcgcgact gagacttcca
     4501 cgggaggggt ggagctcgat ctggcgaagg tttcgtatct gagcagcgcc ggcctgcgcg
     4561 ctttgctgat ggcggccaag gccctgcaaa aacgcagcga acgcctgaaa cttatcaacg
     4621 tcccgctgcc tatcaagaac gtattgaatc tcacaggctt tgcgacattc atcgacgtca
     4681 gcccttgatg gcataggcgc ggccaaggac tgcggcgcgc gcgagacagt atgaagatag
     4741 aagccaacgt ttggcagaga gtttctggca ctccgtcggt cgacatcttt ccgatcatac
     4801 ctaaggccaa ctgcgtttca tcgaattgct acattttttc cgctcccgag gcgctggtgg
     4861 ttgtcgatcc gggagcaagc gccgagcaga cgcggcagat aagccgaatt ctaaccgacg
     4921 ctctaatggt gtcgcagcgc ccggtcctcc tatttcttac tcactgccat caagaccact
     4981 cgcaggaggc ggatcttgaa cttcccacag gtacggaagt cacgcgcttc gcgcacaagt
     5041 cgggagtcga ggcgctgaaa caccgcgacc gcaacctgac taccgcttat ctctatccat
     5101 ggaatccgga aatatgcgcg gcgccattcg acggaatcct attcgcctcc agctcggcgt
     5161 cggagccggc cacactggaa tcgtccggcg gacggcgatt cgagctttac actgagccgg
     5221 cgacgctgcc cgatggcgca acgctgcaac ggcaatggct ttctctcggc gccggtaatc
     5281 ggctcgagat ctatcacacg cccggacatt cggcatgcag catatcgtta agaatcggat
     5341 cgttgctgat gcttggtgat ttgccgttcg ccgccaaccc tggcgtgtgc ggtctcgacg
     5401 gctggaacca cgcagatctt ctgcaaacac tgcgcaacgt cgattggctc ctggaaatac
     5461 ccgacattac cgcgtgttgc cccgggcacg gttactgcgt tccagcgcag agcatgcggg
     5521 agaagctccg gttgatgaga actgaagctt ccaatctgac cgacgtaccg ctgatggggg
     5581 ccgaacgcat tagcggtctt aagatttacg cggatgaact gctcgaagaa acgacggcgc
     5641 tgcttaccat cttttccgga cgcctgtaca ccgtatctta ctgcttatcg atgctcgagg
     5701 aggaggacag cgcggcaagc cgtatcctcg agagcttgga tatcgaccag atcgaccgca
     5761 tcctctcgga ctttcgccgt gtcgccgaag cctttaacag cagcgcgatg cccgaatgga
     5821 ccgtggtgct caaaggcgtt caagtcgcca ggtcattgca aaaagttctg tccgcagaaa
     5881 acgttcggca actcctggat ttttctctgg ttgatcgggc ccagcgacgg ctggatgact
     5941 acttgagcct ggtgcgcggc ttgcaatttc tccagaccga gcaaccggcc gccgtaaacg
     6001 agatcgtaac ggagctactc aagcggacca aggaaactac gatgacacaa tccgctgacc
     6061 tgatggaagc ggtggacgat gatcaatcct tcctgcaggc tcttacgcgc cgattggctg
     6121 cctactcgcc tctgcgagat atccagtttg aatttgcgcc tgccgcccag gagactaacg
     6181 caaacatcgg cgcggagcgg ctcgatgata tattgaccaa cgtgatggaa ggaatggccg
     6241 gaatgggcgt caagcacatc agcattgcca ctcaggtggc atcgggtcag gtcgagattc
     6301 gactttcgag tcccgatcct atggatccgg ctgcgttcgg caagcgccgg ctcgatcttt
     6361 acaacagaac tctcgaatgg ctcggcggcg gcttcgaatg catccaacac cggggcaacg
     6421 ctgatttcat catcagactg ccggcgctga aatcaatgtg aagcggagct gtcgtaaaca
     6481 gatcggcgcc cgcctcgaag atttggatgc gaggatggta acaattcgat tgacatcttt
     6541 cgccaaagat aatcagaata cggctgatca tgcctaagca aacaatcttt ataagttacc
     6601 ggcgccaaga taccgcagcc tacgcggggc gcatctatga tcgcctgtcg gcgaaatacg
     6661 gcgagcataa tgtatttatg gatatcgaca gaatcgagcc gggacagatt ttgtcgatgc
     6721 catcaaccat agcgtcgcgg aagcgggcgt gcttctcgtc ctcatcggac gagagtggat
     6781 caagatgacc gacaaaagcg gcgttcctcg actggaaaat ccagaagatt ttgtacgatt
     6841 agagattctt gctgggctcg agcaaaagac cgttatcgtc ccggttctgc tggccgacgc
     6901 ggagatgccc tctgcgcagg ccttgccggc gccgctgcag ccgttcgcgc gcaggaacgc
     6961 catcgagata agcgactcga gatttcattc cgacgtggat cgattgatcg aagccatcga
     7021 aaaaattttc aatccacaaa gagcggcggt gccaccgatc cgacccgagc agcgaaacca
     7081 aagttcgacg ccatttagta gcgcgagtct acgtcctaac acgggcttca ttgccgcggc
     7141 gattgctgca gtcgcactga tcgtggcggg aatttggtgg ttcacgaccg gagccgagca
     7201 ggaaacgagt tttcaggcag aaccttatat ccagtctccg gctccaaagt cggccgagaa
     7261 ctcaccgcgt caagatacac gccagtctcc cgagcaacaa agtccgcgcc agtctccggt
     7321 gcaacaagat ccaagccaaa cgagtccagc atacaatcct agacagagtg ctccgagaaa
     7381 ttaaccagtg tatcgctgc
//
LOCUS       DASUVS010000198        57996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_954360, whole genome shotgun sequence.
ACCESSION   DASUVS010000198 DASUVS010000000
VERSION     DASUVS010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 57996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57996
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954360"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..817)
                     /locus_tag="VF182_11005"
     CDS             complement(8..817)
                     /locus_tag="VF182_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6437650.1"
                     /translation="MPIDAAHPPSSNLWLARLREVAYNWLGIIGIIIFFLAWEIATRT
                     HILNPFYFPPFSQILAKGYELFANGSIWEHMRFSLTNFSIGFLISVLLGVVIGVPMGW
                     YRSLSKAFDPLLSGIYATPLIALLPLIIMVFGLGPISKIIMTILAAVFPILINTMVGI
                     ANTDHRLITMARAFGAKDSTIFLKVSVPGSLPYIVAGMRVALGRALVYIVVAEQYGAA
                     TGLGYLSSVAAQRFQMAAMFVPIVIIAALGATLNELLKALEQRLEKWKPQK"
     gene            complement(1542..2870)
                     /locus_tag="VF182_11010"
     CDS             complement(1542..2870)
                     /locus_tag="VF182_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017266271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6437651.1"
                     /translation="MSIKELRRVVIAAAVGNIIEWYDFYIFGSLASILAVKFFEKGHP
                     VAAFLSTVALFSVGFLIRPLGAFLFGWLGDKVGRKYTFIVTLTGMGISTAIIGMIPTY
                     AQIGIAAAIIIFFLRLIQGLCLGGEYGGAITYVAEHVSDEKRGYYTGWLQTSPTIGIV
                     VSLAVIIGTRTYLGEAAFNDWGWRIPFIVSLLLVAIAIYIRLSLQETPIFQDIKAKGQ
                     TSINPWREAFMSRNLRYVLIASVVVLGQGCVWYSGQFWALYFLQTVKKLDVLTSSYIV
                     GAALLIATPTLIFWGWLSDKIGRKPIILGGMLLAALTYYPLYTALGRYAEAGNMNYPM
                     SILIVVILVNYVGMTYGPIAAFLAEFFPGNIRYTSVSVPYHIGNGWGGGLVPIITTSV
                     YLSTGSLAWALAYPIGLPLLMFLIAIFVMPETRKHSIWKEGAIEATRHRA"
     gene            3380..4264
                     /locus_tag="VF182_11015"
     CDS             3380..4264
                     /locus_tag="VF182_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6437652.1"
                     /translation="MIIKNPRYDYSPIITREKIAWPDNGRIALWVAPNIEYFHFDMPI
                     RGSGSTHKPDVPGYTLRDYGSRVGVFRIMSVLDKFGIRASVLLNAEVCEHHPEIITEG
                     NKRNWEWLGHGLTNSVSMLDYSPDEERGIIRKVKETITASTGKEPKGWLGPGLGETFD
                     TPDHLAAEGFEYVCDWGNDEQPTPMRVKTGRMIVVPYELGVNDIRVFNRENHTAEQYY
                     RMVCDHFDTLYRDSVSGGRVLCLPLHPFVIGLPFRISYLEKALDYICSHEGVWRTTSG
                     EIAEWYYKNYYKDPGKPA"
     gene            complement(4320..4874)
                     /locus_tag="VF182_11020"
     CDS             complement(4320..4874)
                     /locus_tag="VF182_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX6437653.1"
                     /translation="MSKNITREPGDMADVVQRNIQALLLRRNQEEKTKGVQDRIADAI
                     SRFAGSMTFVYIHLIGFGIWIVVNLGWTPLPTFDPTFVVLAMFASVEAIFLSTFVLIT
                     QNRMAAQADRRADLDLHISLLAEHEVTRLISLVTAIAERVGIEAASDHPELAELEKDV
                     DPEKVLEKIEETESNMTKKAPDQL"
     gene            5127..6572
                     /locus_tag="VF182_11025"
     CDS             5127..6572
                     /locus_tag="VF182_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6437654.1"
                     /translation="MTDGKAKQFAVENIERNREAIALLCDNIFYFAELGMQEFETSGL
                     MMQILEKAGFTIERNPSGMPTGFIASYGSGKPVIALHTEFDATPGCSQAPGVAEPTPI
                     VDGAPGHTEGHNVNGAVMIGAALAIKKTIDEFGLKGTLKVFGAPAEEQLVSRPYFVRD
                     GYFKDVDVALHDHIGKELGTVYGLRQYALISAEFTFKGQSAHAATAPWNARDALDAAV
                     LMDIGWDKLREHLEPTQRSHRVITNGGDQPNVIPNRSTIWWFFREATAEKTRALFEKA
                     KRVAQGAALMTETSYDVNVLSAVWPTRANRTLAEVIQANIEEIGMPRWTEGEQKLVHE
                     VQTKVKAKPTGMPTKVAPLKECVQSVSANDSGEVSWVVPTGLITFPSNIPGVSYHHWS
                     AGVSLATSIAHKGAIAGAKAMAASALDLLLDPQIVAKAKKTFKDEIGDVEYRPLLPAE
                     QKPPLDLNRDLMERYRPKMREHYLRERPEFR"
     gene            complement(6697..7410)
                     /locus_tag="VF182_11030"
     CDS             complement(6697..7410)
                     /locus_tag="VF182_11030"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX6437655.1"
                     /translation="MNLSILIMSLILLSSSVHAQERNRVRPPSVTAQGEAVIIAEPDQ
                     AQIEIGVLTQARTAPEAAKENAEKLGRVLAEIKKLLGKGDEVKTTGYTLNPNYRYPQG
                     GKPEITGYTAANVLRIKTGILESVGKLIDAGMQAGANQINRLVFTLKDEHSAQVQALR
                     MAAQKAQSKADEMAGALGLKVVRVLSLTENERGVRPIIAQTRTVQAEALSSTAPTPVE
                     AGTIEVRSSVTLTAEIGPR"
     gene            7864..9183
                     /locus_tag="VF182_11035"
     CDS             7864..9183
                     /locus_tag="VF182_11035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6437656.1"
                     /translation="MESTIEQAHPAAKQKFFYGWYIVGVGFLANVASAFSLASTMSVF
                     LKPLTADLGVSRGVFSLLRSGEGLIAAFLAPVIGTLMDRYGGRWLMVFGAIIASLGYI
                     VLSHVDNFFQFSIVRLSLITIGDCFMGYIVINLAIAHWFSRKRGRALALTSMGIGFAK
                     VGMPVLAASLIVWLGWRQTWLVFGIVTLVLVVGPALLIVRRSPEDMGLHPDGALSSAA
                     KDEPVTHSAKTPSITSKAIRDDVEWTRQEALHTRAFWLLVVTFGISSIGVTGLNLHVF
                     PYVNDLGHPPVVAATVMSVIASMQLASPIAWGILAERIDTRFAATLRFVVQGIGLGLA
                     VITDNLFCLYAGFLLYGIGLGGNMVLPDILWADYFGRRSLGKVRGLGLLISQTVAALG
                     PPFFGFLFDLTGAYDLSFMLFGAALITSAFLSLMLRPPVKTCEQANS"
     gene            9207..10481
                     /locus_tag="VF182_11040"
     CDS             9207..10481
                     /locus_tag="VF182_11040"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX6437657.1"
                     /translation="MSLQTVHLDFAELTADQQVGRLKEQHALLRGKGAVVRARVHDLP
                     VRQYIALLERGYRVGLEQEGSGFTLTLDPNGSTPRTGLRGAHSVVSHDDGRIYANTTD
                     NRVAVIDGGTRRLKKHIPTGDDPSHLELSADGKRLYVANSGSNDVTIIDTTADQVPAT
                     VSTGKRPLLPCIAPNGNAVYLPSGPERTVTVLGADGEFRARIAVGIAPHDIAVSPDSS
                     WAYQPNSASHTVTIIDGRNYSVAGEIKVGLGPGHIAFDPENRFAYVANTVSDDVTVID
                     VGTHEVVASIPAGRGAHLPVIGPDGRYGYVANFASDNLTVWDCNNHQVIATIPVGIYP
                     HFFALSTDGDWLVVSNTGESSVCIIDARRHEIRARLQVGPAPAHLAFSPDNECAFVGC
                     ESSDEVVVIDLRKQTLVESVKAGQRGPEAPWS"
     gene            complement(10747..11766)
                     /locus_tag="VF182_11045"
     CDS             complement(10747..11766)
                     /locus_tag="VF182_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437658.1"
                     /translation="MSIIGKFIQRSLFTLAFILFASSAWAQSASMDELYNKALKEGGT
                     LNFYGTLAQISADKVLPAFEKRFPGIKINHVDATSNKMVARAVAEARGGKTIGDVLQI
                     NLDDTVHAYEQNLLTEFAVPEAKEYPDGLKGSYWIASDMDFYIAAWNTNLVKKEEEPK
                     EFDDFADPKWKNRLIGEPNDEEVLIGLAKYKFKNDDKAIALMKKIAANNPEFHRGHSN
                     LAELLSSGQAAACFTCFSQHFPARIKKGSPLNYMLTEGVAGIGATALFKNAPHPNTAL
                     LYIRWISSQEGQKMMAIAGRTPAHPKVERVNKTRTEKIYPIGVADIKEFSKYQKIWRD
                     IFQLR"
     gene            complement(11916..14330)
                     /locus_tag="VF182_11050"
     CDS             complement(11916..14330)
                     /locus_tag="VF182_11050"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6437659.1"
                     /translation="MSERVSVSEETNSTRAVRGYCALCTAHCATIATVENGRVIALEA
                     DHDHPNGGVICVKGKAAPELVYHPDRLDYPLKRTRPKGAADPGWQRVSWEDALDDIAQ
                     RLSAIRDQHGAKALALGKGTRSGTSVADVERWLGRFLYLFGSPNWVSTTHVCNWHKDT
                     GFSYTFGSSIPTPDLAHSKSFLLWGHNPSSTSLILAHDIVAARSRGMKTVVIDPRRVG
                     IGSQADVLLQPRPGTDGALALALIHCLVEESWYDAGFARQWTNGSFLLNTATDQLVRE
                     ADLVVDGARNRYLVWDEIKNQAVIYDPSTGGYERDNIQAALFGPRALRGKSGEEIPCK
                     PVFEKLGEIASAYAPERSEKITWVPAKNVWQTALLLAHNRPVSMYMWNGLGQHTNATQ
                     TSRAIASLYALLGDYDRPGGNASFPGLRVNDVGGKEFLAKETAAIRIGRERKPLGPPA
                     KPGNCTAYDIFTAILEQQPYPVKAFLNFGSNTVLSTADSSRAREAMVALELGVAADLF
                     MTPTAELCDYVLPATSFLEMASITTSFEHRPAGKTHMQYRPAVVKAAGERRSDTWIIF
                     ELAKRLGFGEKFWNGDIEAGYAYELEPTGVTLAELKQAPGGISLPAQPAYQKYAGTNK
                     DGAVRGFATPSKKVELYCETFAAHGYPAMPEYIEPALSPISRPDIAAEYPLVLTNAKF
                     TTFVHSQQRALPSLRKISPHPTADIHPKTAGLYGINNKEWMIVESPRGAIKVKARVTT
                     NIVPGVICCQHGWWQDCKELELPGYDPYNSNGANPSTLIGTDITDPVSGSLPHRSYLC
                     RVRPAG"
     gene            complement(14454..15734)
                     /locus_tag="VF182_11055"
     CDS             complement(14454..15734)
                     /locus_tag="VF182_11055"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6437660.1"
                     /translation="MDGSTTEQQAVLKLIDEQELVELAVAMGNIKAPSGYEQPMADFV
                     LQWLQANGFDDSYQQLVSEGRSNSIGVLRGTGQGKSLIFNSHMDSEQGMPMRLDEEPP
                     PGPTASVDQKKKRIFGLAVQNDRGPMAAFMIATKAIKNSGIRLAGDILMTMVVGEIGM
                     GPVDEFRGARFIGKGYGSRHAVTHGINGDFALIAETTDFGVTWIEAGAAYFKVTVDGR
                     SFYTPRLPERGEFKDNPNALIKMIAVIEAIEKWAVEYESKYTIEYPVGKMVPKVSIGA
                     IRAGAPYKPSTTPKTCSIYVDVRVPPPLEFNQVERELRDVVQSVGLGGTVECFMARKG
                     YEGKNVEALIDAVRNAHLLVRGAAPPPVSTPETSMWRDINIFNEVGIPAATFGMPRKS
                     APDAVERFVEIKDIVDAAKMYALVALEICGARRN"
     gene            15885..16679
                     /locus_tag="VF182_11060"
     CDS             15885..16679
                     /locus_tag="VF182_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6437661.1"
                     /translation="MKEIPALELRDVSYKANGKTILQGINWSVQSGEHWAILGPNGSG
                     KTSLLKIACGYIWPNAGGEVFRRGRLLTNLPELRKSIGWVTSTLTTQIPKHEKVIRTV
                     VSGKFAQIGLLEQFGGNAGKRDYATARRYLSEMGCDHLREQEFGVLSQGEQQKILIAR
                     ARMTRPYLIVLDEPCAGLDPGARENFLATLGRIARQAKIPALIYVTHHIEEILPIFRK
                     TLILKDGRVVHSGNTDRVLKGDVLQDLYGVGLSIVKKKRRCWPVVR"
     gene            16939..18423
                     /locus_tag="VF182_11065"
     CDS             16939..18423
                     /locus_tag="VF182_11065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6437662.1"
                     /translation="MISPQSYEPNTQRHRWWSLAVVSLGTFMVTTDIGLLSISLPVII
                     TDLHADLALAGWIALIYALVTASLYLPCGRLSDMIGRAKVYRVGFFLYAVTSLIAGFA
                     QDAWQLILFRGLQAIGSALIMTNSFAMVAAIFPPEERGRAMGIAGGTVSALGYTLGPV
                     IGGVLTYALGWRSNFFLTSSLAFIGFLAACYLLRDDADASARQGVKESFDFAGAVSFA
                     LAICALLLGLTAGQRAGWSSVLILSELGFGLATLIFFIWWEAGNRFPLLDLRLFQIRA
                     FTFGNIARLLSFMVISLNNLAMPFFLQLAMGLDPLHAGLLVAPTPLALALLAPLAGWF
                     SEKVSARLLTAVGLTLKAIACVLLAYVSVTASSFDLVLRLGLLGLGLGIFQTPNNNSL
                     MSSIPRERLGVGSSFLSVVRSLGHSAGTALATAIISARLLAVTGQEAIDDLKNTASIG
                     AGAVLPAFMEGFRYAFLTAAALCVVGAVISALSVENDRSTARIR"
     gene            complement(18410..19231)
                     /locus_tag="VF182_11070"
     CDS             complement(18410..19231)
                     /locus_tag="VF182_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007114061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HEX6437663.1"
                     /translation="MSRTLIQFSGWLALAYIHGYTLALIISPMIIIFSLLFVLGLPLY
                     TVLSGQVPLGRDYRTADRSSAGIAPPAETTPEAVVQVYGARALNWRGIFGVHTWIATK
                     PADATQYTIHHVIGWRAYRNLPVVVSAPGIPDGRWFGNEPELLLDIRGDVAAGAIPKI
                     IEAAATYPYAKDYLLWPGPNSNTFMAYVGRQVPELKMDLPATAIGKDYPINGSVIDRT
                     PSGTGYQLSLLGVFGITLARKEGIELNLLGLSFGVDILKPALKLPFVGRLGVSEF"
     gene            19397..19867
                     /gene="ispF"
                     /locus_tag="VF182_11075"
     CDS             19397..19867
                     /gene="ispF"
                     /locus_tag="VF182_11075"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX6437664.1"
                     /translation="MRIGHGFDVHRFRRGRKLILGGVEIPHKAGLIGHSDADVLLHAL
                     MNALLGAMAEGDIGSHFPDSDARYKNIASAKLLAEVLAIMRRKRFKLINVDVTLMAQE
                     PKLAPYYAAMKTNVAGYFKVDENRINIKANTMEKLGWAGKGEGMAATVVVLLAR"
     gene            19935..20282
                     /locus_tag="VF182_11080"
     CDS             19935..20282
                     /locus_tag="VF182_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437665.1"
                     /translation="MISNLPNLAPQAREQEIEKVAQELGRLIQGADPEQRNDLKEMAF
                     ALIREEFIHKAEDQESQPGSAPAPFNPLGTGVLIFCVGAGLSFIFGPVGLVLMLAGVI
                     FVTWGAVISWIKG"
     gene            20290..20808
                     /locus_tag="VF182_11085"
     CDS             20290..20808
                     /locus_tag="VF182_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX6437666.1"
                     /translation="MPEEEKKKRGIWSRRDFFTRLGWAGFGVFGGLSLLGFLRSAFPR
                     VLFQPPSTFKAGSPSDYAIGEVSEKYKQDQRVWIVREDTGIYAIFAKCTHLGCTPRWL
                     ATEAKFKCPCHGSGFFKSGLNFEGPAPRPLDRFQITLGDDGQLVVDKSKVFKMAPGGE
                     PDEQHADSILKV"
     gene            20820..21590
                     /locus_tag="VF182_11090"
     CDS             20820..21590
                     /locus_tag="VF182_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX6437667.1"
                     /translation="MSLLEKIKSDIVESHVWKSIFRHGYDDTPRNRVMMVTSNVFLHL
                     HPAKVRRHAVRMRFTWCMGGLTFLMFLITAVTGVYLMFYYRPVAEYAYADMKYLETDM
                     PFGMLMRNMHRWAAHAMIVFVWLHMFRVFLTGSYKPPREFNWIVGVLLLVLTLLLSFT
                     GYLLPWDQLAIWAVTVGSNMARATPLLGYEGPFAEFVGANPIYDARAFLFGGGEIGPH
                     TLLRFYILHCIFIPLVASILMAVHFWRIRRDGFSGPSL"
     gene            21614..22381
                     /locus_tag="VF182_11095"
     CDS             21614..22381
                     /locus_tag="VF182_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX6437668.1"
                     /translation="MAEAVRERRVPVDDKIHTWPHLVRMEFLVALFVIVALTLWAMMV
                     DAPLEEAANPTRTPNPSKAPWYFLGLQEMLVYFDPWHAGVVLPTLIIVGLMIIPYIDI
                     NPKGNGYYCFKDRKYEILTFFLGFHVLWMVTIIIGTFFRGPGWNLFWPWQHWDAHKVV
                     ALTNVDLPYLFGFRDYWWSAIFGLMVIGAYFVFGLIAFYYWVLKIKGREFLERWGMVR
                     FTLTAVIFVLMLSLPAKMFLRLVFNVKYILVTPWINI"
     gene            22381..22548
                     /locus_tag="VF182_11100"
     CDS             22381..22548
                     /locus_tag="VF182_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437669.1"
                     /translation="MFYVAKILQALGLADVGYALLTGIMKNSSMAEEIVLALIGLGVF
                     SLGRALERKVA"
     gene            22559..25258
                     /locus_tag="VF182_11105"
     CDS             22559..25258
                     /locus_tag="VF182_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX6437670.1"
                     /translation="MAERGIDPQEEKKSYGSVFLLGIALLVALSIWAFWDDNITRRPW
                     KSFQSQFFRLDYNKAKAAYEEEDKKLQADPAYQELNKKLAAIQGSIEDGELGEKLATL
                     EKQESEASVRFGEIDQEVKFIKSELEEAWYEHDHAIQQKRDPKSYAEHIQELEKEKAK
                     LDPELQAARLKRDNIRDEIKKINSSSKELQTEVSKLSAERDRWRRVMENATFKLGPVS
                     FYKIPKIQQTVMEEFDRNRFNQPIARVDRCQTCHLAINRAGFENEPHPFKTHPRREVL
                     LADSAHPPETFGCTGCHEGQGVMVNGVKQAHGEVHLWEFPLLRGVKTQSSCTSCHLDV
                     QKLQDVPLLTEGQRLFEQVGCTGCHLVQGYDDIPKIAPSLTKISAKVDPSWMVRWVEN
                     PHNFRPRTRMPNFQFKPEEALAISAYIWSQSKEEAEPWLQQNPLPAGIREGDSNEAAR
                     GKKLVESIGCKGCHGFAEGEFSTPLGKTKDLVPNLKDIAAKTGPQWMFHWIKNPKGFS
                     PETKMPSLRLSDDEARAITSYLTTLGTKGETIAGLEEKLANPQNIKDGEALVRKYGCP
                     GCHDIKGMEKESRIGVELTTFGSKTVEELAFGNRTDVPLTWDDWTFHKIKTPRGYATE
                     RVEQLMPQFDLADEDINALRVLLGGFRERKVGKRYLADHGQRATQVVEGRRLMQQYNC
                     VGCHEIENRGGFVRKYYGENIAAAPPQLNGEGEKVQSNWLFGFLKAPFTLRPWLTIRM
                     PTFGFSDQHTTELINYFNGLSRVESPFAYFDEKNIPPDHLDAARKLVSEEYFNCFSCH
                     VRGGKNPEGPPEGWAPDLAMARQRLNPNWIIKWIQDPQKIQPGTKMPSFYPGGPDDIL
                     GGKDDRQIEALRDYVMSLGRTGGATAATPSPAVEESRAKAVKR"
     gene            25313..26026
                     /locus_tag="VF182_11110"
     CDS             25313..26026
                     /locus_tag="VF182_11110"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX6437671.1"
                     /translation="MTKFGSLVLSAAIFALPASSLAQYKAGDVKDGGSVSGTVKFKGT
                     APEPKKLDVSKDKEVCGKTPKVDPTLVVNNGNLANAVVTITDIKTGKKMDAKKVTLDQ
                     KGCEYHPHVLAFPAGSTVEILNPDGILHNVHSYSKVNSPFNMAQPKFKKNLDVKIDKP
                     EAIEVKCDVHGWMHGWLVATESPYVAVTDNSGNFKLTDVPPGSYTMEVWHEKLGKNTQ
                     KVTVKAKEDAKVNFELAAK"
     gene            complement(26109..26795)
                     /locus_tag="VF182_11115"
     CDS             complement(26109..26795)
                     /locus_tag="VF182_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6437672.1"
                     /translation="MGSDLQKLKKDVVSACRILSRQKLVQGFGHVSARIIDSDQFLIT
                     PRISLALVREAELLTMDLDGKVVAGDHPAPFEAALHASIMKMKPGINAITRIHARVAN
                     MFSVTNRKLEPVHNHGSFFAGGVPVFYLPDLISTPQLGEDVASTIGEQPAVLLRGNGQ
                     VTVGRTIPEAVMMAIYLEEAAEILYGALQIGTPIPLTSEESAKRQVEALPPVDLERAW
                     NYFKDQIDIE"
     gene            26867..27136
                     /locus_tag="VF182_11120"
     CDS             26867..27136
                     /locus_tag="VF182_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437673.1"
                     /translation="MKIKVESISDAKGQETPSRFMLGIRTIEIQAVADRWYGEQACYF
                     RVLGSDENSYILKGPLEDGSWELVSFTHKDSRGTELEYEGKRELQ"
     gene            27293..28657
                     /locus_tag="VF182_11125"
     CDS             27293..28657
                     /locus_tag="VF182_11125"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6437674.1"
                     /translation="MSKQHLVAVIGAGPAGIYGTRKLAEAGHRVVLLNRDIKPGGLAE
                     YGIFLDKEKMKTGLRKQFKKILADPKVFYLGHVSVGHDQALTLNDLKEIGLSAIVVTA
                     GAQGTKKLGITGEDSIGVYHAKDVVYHYNLLPPFSQRNFEIGRRVAIIGMGNVMVDVA
                     NWLLNYKKIDQVVVVARRGPLEKAYDDSEFEVIEPYLDNLDMKEEIERIRPQLTAVGQ
                     NIDQISEKFVKQGEPAEGRRLQFRYLCSPTKVIADDRGGVAALEVEENELILQDGKTV
                     AKGTGKQTQIKVDCVIYAIGDQVDASLGLPFSRGVFVTNPRKVLADPDPAAYQPYDPE
                     AATVLDGIYVAGWSRNASVGLVGVAKQDAERGIKVVNAYLSAKDGFTPGELEAKIEAI
                     LKILDKKRVSVVTKEDIEILESVEHEEARNRKTWDFKFMTDDQMLQAIRERKARADSP
                     LKFS"
     gene            29331..29528
                     /locus_tag="VF182_11130"
     CDS             29331..29528
                     /locus_tag="VF182_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437675.1"
                     /translation="MIKAENSIKSSENFDDFTEARASTNARRSNNWKVGSLETFLQSL
                     MARLNPHNGPNRLKEWSELSR"
     gene            29683..30675
                     /locus_tag="VF182_11135"
     CDS             29683..30675
                     /locus_tag="VF182_11135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HEX6437676.1"
                     /translation="MMDTEGNIKRSVIGVLLCSVLLGFAASWVWSAATDAPKPAPATY
                     VGNEVCQACHAPQFEKFSTTLMGKIFLHNPRNETEKRACENCHGPGSEHVAAGGGKGV
                     GGMITFRKDSGESAEVQNEPCLGCHQRGIQTFWKASTHAGRGLTCVSCHTLMEKTSGK
                     QLTKQTEVTPYGVNRAEIEVCGQCHLQRKAQLMRSSHMPMREGKMVCSDCHNPHGSPN
                     PAQLRQTSVNENCYTCHAERRGPFLWEHPPVLEGCNNCHEPHGSVNDRLLKVTDPRLC
                     TQCHIHTRHPATPRPLKPLSPTVGPSVFAFNRSCTNCHSQVHGSNHPSGKFYTR"
     gene            30694..32997
                     /locus_tag="VF182_11140"
     CDS             30694..32997
                     /locus_tag="VF182_11140"
                     /inference="COORDINATES: protein motif:HMM:NF023282.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family decaheme-associated outer
                     membrane protein"
                     /protein_id="HEX6437677.1"
                     /translation="MICKKTILHLSIGIATLLLFAARGAAQMEVGDYTLSGAVEAGAM
                     PSHRSGNISKLEEYRDLPETLVVPQLQLFIDSKRKDFYLEFDSIKTGRDDQSYRLRTG
                     RYGLLDFEFVWDQIPHLFNVDNARTPFNMKDGRYTLANKPAGTNGADVRDWVNANAEP
                     VDLKLYNGLARFNVRYTPTPGWTFNGSYGSQNTNGERAFGVYIGPSPGSYNISELAEP
                     IDYQTHNIELGGEYAGDGWSLGLKYNGSLFHNNTSTLTWDNPLNISGIGGACVDSAVY
                     NQNTGTGPCRGRVDLYPSNQAHNITLSGTASLPFKTNFIGAFSYGWRLQDDPFLPFTI
                     NSAVTQPTISRRSLDGDVRPTMINATFVNNFFRDVGLKAAYRYYDFDNRSKRVLLPDG
                     FIVNDQGVANGTELLSFPYSYAKQNIGLDASYNVTRWLTAKLGYGWERMHREYARELL
                     NSNEHSFGPTFDIKPSAWTLFRMSYRRSIRDAHDYDAGRNVAVEIGETPEDVRLSRLE
                     ELRKFDEAARDRDRFTFFSQVSPLENLTLHGGFDFTKDRYPRTVIGVQRDIDYAPSLG
                     FIYAPLEWLSVFGDYNWERFDWRLQSIARNTGPGGCPDLEARTSENCPAATWRSRGND
                     EIHTVKIGTDMQVVKNILNLRLQYGFSVGSSEVNSSGNSPASGDTGIVPATDYPTIVN
                     RWHEFVAGMEYVLSKNLSLKLGYYFNSYSSKDAGVDIMRVWMGDVETPAGGPLFNTST
                     GRSIFLGDRGKGSYQAHIGFLGARVKF"
     gene            33313..34578
                     /locus_tag="VF182_11145"
     CDS             33313..34578
                     /locus_tag="VF182_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437678.1"
                     /translation="MNLIGAILLTAVVVLCSDLTAGAAETKPQEDSCATCHLALGIEK
                     LTQPAELYKADVHASKAFGCAACHGGDPNIMGLEAMDRKKGYIGKPAPIQVVEMCGKC
                     HSDANFMRRYNPSLRVDQVAEYYTSVHGKRLQDQKDPKVATCASCHTPHAIRPPNDSR
                     SSVHPTRVAKTCGSCHANAEYMASYKIPTDQLEQYQQSVHWKTIINKSDLAAPTCNDC
                     HGNHGAAPPGVSSVVNVCGQCHAVNAELFNKSPHSKVFAQMGISGCATCHGNHAVVET
                     SDAMLGVGDKSACAGCHPPGSSGGALAASMLGSIDRLKAGYEKAFAVLAKAEHAGMEV
                     SQPLFELNQAKTTLIKARAAIHGFDQTLLDKEVEPGLQAIEKSYARGVRAMSELQFRR
                     RGLVVSTLIILALVIGLIFKIRQMERKSK"
     gene            34597..35058
                     /locus_tag="VF182_11150"
     CDS             34597..35058
                     /locus_tag="VF182_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX6437679.1"
                     /translation="MAGNERSIPLDNGRRRFLNWFLGTTAGAFFISVTYPLSRYLIPP
                     AVEESTAHTVTLSLKPQEVKPNSGQIFRFGSQPAILIKTPNDELKAFSAACTHLACIV
                     QYRSDISHVWCACHNGHFDLNGKNIEGPPPKPLEEYIVNVRSDQIVVNKRA"
     gene            35065..36162
                     /locus_tag="VF182_11155"
     CDS             35065..36162
                     /locus_tag="VF182_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEX6437680.1"
                     /translation="MEMPTPKHGKLWLWFDERIGLSDFAYLAKKKEIPLHRHTFWYYF
                     GGMTLFLFMVQVISGILLLLYYRPSAAEAFESIQFLMTEVEFGWLVRSIHSWSANLMI
                     FAMFIHLFSALFLRAYREPREITWLSGGGLLGLMLAFGFTGYLLPWNELAFFATRVGT
                     EIPGIVPVVGPFLRRLLRGGDDVTGATLTRFYGIHVAILPAIVTVVLGLHLFLVQKHG
                     MSRPPSGETGEPGKSTMPFFPNFFLRDLVGWLCALAVLAALAAYFPAELGKKADPFQP
                     APVGIKPEWYFMAMFQTLKLLPAHIIGIEGELLGVLGFGLLGIILVLMPFLDRRHALK
                     GWSRWPAAFGVAMIVYAIVLTYLGYTMSPTK"
     gene            36365..36694
                     /locus_tag="VF182_11160"
     CDS             36365..36694
                     /locus_tag="VF182_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6437681.1"
                     /translation="MRQKINVAIAGLAIWAGTIVAAVAGDADSGKALYEKSCVGCHGP
                     DGKGNEKMAKLLGDKGLNIVGDETKKKSDEQLMKVLSEGTGKMPASKLSREEQKQVLS
                     YVRSLAK"
     gene            complement(36768..36959)
                     /locus_tag="VF182_11165"
     CDS             complement(36768..36959)
                     /locus_tag="VF182_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437682.1"
                     /translation="MTEERSRLHRKLKIAGYLLLAGLAVEGITLHWSHPTSFLLFIIL
                     GGVLVLAGITIYLIELVTA"
     gene            complement(36956..37564)
                     /locus_tag="VF182_11170"
     CDS             complement(36956..37564)
                     /locus_tag="VF182_11170"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEX6437683.1"
                     /translation="MADYAVATVIVLSIILIGVLLTRPSITAAQGGKIVAFIAFFILP
                     IVATVLGASIHLEQSTSTEFCLSCHVMEPYGKSLLIDDPSYLPAAHFQNNRIPKDHAC
                     FTCHTDYTMFGDVNSKLRGLRHIYVYYFKTPPAKLALYEPFKNRECLHCHANGRSFEA
                     TSPHTEMREALGTNDMSCLTCHTKVHDVANIDQKKFWKGPGT"
     gene            37830..38159
                     /locus_tag="VF182_11175"
     CDS             37830..38159
                     /locus_tag="VF182_11175"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6437684.1"
                     /translation="MKYTVLTTAISLALAWPMNQSLAADADVGKTAYEKNCAGCHGAD
                     GKGNPALAKTLGEKELNIVGKETMQKSDEVLLKIIADGAGKMPPSKKLTKEEQKSVLQ
                     YSRSLAK"
     gene            complement(38449..40731)
                     /locus_tag="VF182_11180"
     CDS             complement(38449..40731)
                     /locus_tag="VF182_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX6437685.1"
                     /translation="MAREPMPISSGWFQGAVLTYLFGFTVLGVLAYLVYRDQPPIPAR
                     VLAEDKVLFTREDIFAGMNVFQRYGLMEYGSVYGHGAYLGPDFTAEYLHNTSEFLIKS
                     YAGAPMGGLSSRERVIAELHKNTYNPADDSLVWTGARAAAHRMMEEHYQSIFFAKKTR
                     GGAQAEWISNPDDIRKLTAFFAWTAWTSATDRPGYSYSYTNNWPPEPLAGNFITADAI
                     TWSVISIIGLLGGTGLILYFFGRYDWLGWSETETEPVRFRSIDQVAVTPAQRAIVWFL
                     FVSSLLFVLQTLTGGLIAHYRAEPEGFFGIDFSTILPFNIMRTWHVQLAIFWVSASFL
                     ATGIFIVPLIAGRERSGQGPLTIVLLIAVAIVVFGSLAGEYLGARGWLGEGLWFWFGH
                     QGWEYLDLGRLWQILLVVGLFLWVFILFRGLRGTLREHHFGNMPWLLFYAALAIPAFY
                     AVGLLASPREGFVVNDFWRFWVVHLWVEDFLELFTTVMVAYMFVLLGVVRQTTALRVI
                     YLDIILYSLGGVIGTMHHLYFSGTPVAHLALGATFSAMEVIPLLLLSLEAWSFIKSGE
                     QKTVERYHLHRWAVWFLVAVGVWNFLGAGVFGFLINLPVVSYYEIGTALTANHAHTSM
                     MGVYGMLAAGLLLFCLRYLTRADRWSDRAAKISFWSLNIGLAWMAFVNLFPLGIVQLY
                     YAVDIGYWYARSLAFLDLRWVNVLEWARLPGDALFIIGGALPLLWLCWQALRYPNPRR
                     IEAEAKLPQMLYTYDTEPGK"
     gene            complement(41094..41972)
                     /locus_tag="VF182_11185"
     CDS             complement(41094..41972)
                     /locus_tag="VF182_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6437686.1"
                     /translation="MREPHNVSLLSRILGATDLSPRAVKAVARAVQLADEHRATLTIL
                     HVLPDALGDEASKRQIVLQVEQYLHRKVIKLSPDREKTVSIEVTTGTAFVEIIRRARE
                     KAVDIILVGAHGEQFINKLRFGTTVERIVRRGDRPVLVVKRRVRGPYRTVLMATDFSD
                     QSGEALALAMRVAPSAKYVLLHAYQGIEQQLWRSDFRKSDILRYRRELANQSQEKLKH
                     FIRKRGLDNTSFVRLVRYGRAPQVISSAARRLRPDLVAVGSAGRTGLPHVLLGSVAEH
                     VLREVSCDVLVARPAR"
     gene            42686..46294
                     /gene="nifJ"
                     /locus_tag="VF182_11190"
     CDS             42686..46294
                     /gene="nifJ"
                     /locus_tag="VF182_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEX6437687.1"
                     /translation="MNQRTITVDGNEAAASVAHRMSEVIAIYPITPSSPMGELSDEWS
                     NQARRNLWGNIPEVIEMQSEAGAAGAVHGALQAGALATTFTASQGLLLMIPNMFKIAG
                     ELTPFTMHVAARTVATHALSIFGDHSDIMACRPTGFAMLGAASVQEAHDFAAIAHAAT
                     LESRVPFLHFFDGFRTSHEVSKIHELSDDDLRTLMPEAPIAAHRARGLTPDRPVLRGT
                     AQNPDTFFQAREATNLFYRACPAIVEEIMTRFAARTGRRYGLFEYVGHPDAERVLVIM
                     GSGAETAHETVEFLAARGEKVGVLKVRLFRPFAISAFLSVLPQSVRAIAVMDRTKEPG
                     ALGEPLYQDVVTALAEARADGGSPFTVEPRVVGGRYGLSSKEFTPAMVKAVLDNLAQP
                     DPKNHFTIGIVDDVTHTSLPYDAAFETEPADIATALFYGLGADGTVGANKNSIKIIGA
                     ETDLYVQGYFVYDSKKSGATTVSHLRTSPRPIRSAYLIQKARFVACHQFEFLDRIDVL
                     EHAADHSVFLLNAPGGVDLLWERLPREMQQQIIDKHIRFYAIDAIAVARQAGMGGRLN
                     TIMQTCFFAISGALPREEAIVQIKHAIEKTYHKRGPEVVKRNWEAVDLTLAHLHEVSV
                     PEVVTGTHARPPLVSALAPDFVQRVTAVLMAGKGDLLPVSAFPVDGTWPLGTAKWEKR
                     NLALEIPVWDTSICIQCNQCALVCPHAAIRAKVYDADALATAPPTFKSAPYKGLEFKG
                     RQYTIQVAPEDCTGCNLCVTVCPAKDRTNPRHKAIDMHPQAPLRDAERDNYEFFLKLP
                     DPAHSDLPKVDHKTSQFLEPLFEYSGACAGCGETPYLKLLTQLFGDRVLIANATGCSS
                     IYGGNLPTTPYTTNREGRGPAWSNSLFEDNAEFGLGLRLGVDSHSRAARALVEHQSGR
                     IGDALAGELLEADQSTEAGIHAQRERVIALRQVLARLTTPEARRLDALADYLVKKSVW
                     LVGGDGWAYDIGYGGLDHILASRRDVNVLVLDTEVYSNTGGQQSKATPLGAVAKFAAA
                     GKTVQKKDLGLLANMYGHVYVARVAFGAKMPQTVQAFLEAEAYPGPSLIIAYSHCIAH
                     GYDMAQGAAQQKLAVNSGVWPLFRFDPRRIAKGEPPLHLDYGPPKAQVAEYMRNESRF
                     RVVERTDSVRFKEFLKESQAAAQQRYAVYQQLAGITVPQVKVTIDDQDTPAAENED"
     gene            46296..47288
                     /locus_tag="VF182_11195"
     CDS             46296..47288
                     /locus_tag="VF182_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HEX6437688.1"
                     /translation="MDLSTQYLGFTLPHPLIAGASPLSDDLDSVKRLEDAGASALVLR
                     SLFEEQITHEQMAEHFHLDAHAESFAEATSYFPGPDAFALGTEQYLEHLRRVKESVRI
                     PVMASLNGTTLGGWLEYARLMAQAGADALELNIYRIATDPEISSGDIERQTIDIVRHV
                     KQAVAIPVAVKLSPFFTSIAHVAREIDQTGADGLILFNRFYQPDIDAEELAVSRTLHL
                     SDSSELLLRLHWMAILSGRIRASLAVTGGVHTALDVVKATMAGAHATQMVSALLAHGP
                     IRIRAVFEDLVAWLEEHDWNSLGEMRGNMNLLKVPDPDAYERANYMLMLQSWRV"
     gene            47386..47571
                     /locus_tag="VF182_11200"
     CDS             47386..47571
                     /locus_tag="VF182_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437689.1"
                     /translation="MIGVIDRIYHMSEGRIISHLSRQSGKRKEIKCMPDWSWFIAAFV
                     VYIISVRWVLPRFGVPA"
     gene            48012..49406
                     /locus_tag="VF182_11205"
     CDS             48012..49406
                     /locus_tag="VF182_11205"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6437690.1"
                     /translation="MDQPSIKFLGAAGTVTGSKFLVSYNGTEILLDCGLFQGLKELRL
                     RNWSPLPVKVQALRAVILTHAHIDHSGYLPKLIKLGFRGDVYATSATCDLLKILLPDS
                     AYLQEEEARYANRRGYSKHRPALPLYTVEDAERALALLRPVSYADEIELAKGLSCHFS
                     RVGHILGAAAIRIGFDTANGKRFLVDSGDLGRYGRPILKDPDPIEHADWLLVESTYGN
                     RTHPTNSEDELAKVIKKVAVGMGCLIIPSFAVGRTQEIIYSIRALEDQGKIPPIPVHI
                     DSPMAIDATDVYCAHSEEHDLDMKLLSDAKLCPLSSKKFYIHRTAEESKAINDLAGPL
                     IIISASGMVTGGRVLHHLKRRLPDDKTTVLLVGFQAEGTRGRTLQSGKNEVKMLGEII
                     PVRAEVKTIDGFSAHADQDEILRWLRSFKKPPRRTFVTHGEPPASQALHTVLRDELKW
                     NTEIPSIGQTVVLQ"
     gene            complement(49484..52225)
                     /locus_tag="VF182_11210"
     CDS             complement(49484..52225)
                     /locus_tag="VF182_11210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6437691.1"
                     /translation="MASVHPRYLPVPYQDSAESGRLILRDGTVAEIRLAQPKDCQAMM
                     NFFIGLSGESRRRRFFGFGRPSDKLINAFCDSSDVRKQLSLIVTRTGAAGRQIIATGN
                     YVALDDNTAEVAMAVDDSFHGKGIGTLLLERLTLLAVRSGFRRFRALTLSDNQPMLDV
                     FLQSGFECHRKHSDDYVEIDLSVSPNELSVTRAELRDRASTIASLQAFFRPSSVALVG
                     ASRNPARIGGRILNALLEAGFSGRIYPVNPHMVSINDLTVYPTVAAIPQPVDLAIIAV
                     PPEAVLGVIDDCAKRQVRALVVITAGFAEAGNDGVVLQHQLVGKVRGYGMRMIGPNCL
                     GIMNADPAVSLNATFSPLFPAPGGIALSSQSGALGLAVLALARERQIGLSNFVSVGNK
                     ADVSGNDLLQYWEEDKQTQVILLYLESFGNPRRFARIAKRVARRKPIVTVKAGRTSAG
                     HRAAGSHTAALAASDVAVDSLFHQTGVIRADTLEELFDLAMALSTQPLPGGRRVGILT
                     NAGGLGILCADSCEANGLIVPELDDRTKTQLKAFLPAAASVTNPVDMIASAGTEHFTK
                     ALEILLPSAELDAVIVLYIDVGLADTNSMAKSIASGVAVLRKTVEAQKPVLACVLSAA
                     ETRMQIKADGENIPNFPFPESPARVLSKLVNYSEWKKQPLGIIPDFDDIEPAVARSLC
                     REILAERGAGWLSAEETRQVLTAMALPLTPYGLCRTAEEAVQAARKIGFPVALKLASR
                     EIIHKTEFGGVQLNLQDGDAVRRAFEYVSDQLAKREMSKAMDGAIVQPMISGGVELMI
                     GVSQDPSFGPLIAFGLGGIHVEILRDVCFRVTPITDRDAAEMIRSIRGYRLLQGYRGH
                     RPADIPAIEDLLLRVARLVEDVPEIGELDLNPVIALPPGGGCQVVDARIRVNPESIR"
     gene            complement(52411..52665)
                     /locus_tag="VF182_11215"
     CDS             complement(52411..52665)
                     /locus_tag="VF182_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437692.1"
                     /translation="MMITNKNEEYYERLRERRGEILRTLDYLNKEQRTLAENKHSIDR
                     AAYESRCHLLDSLADWYANETMRIDQALMRSREIEKREFS"
     gene            53276..53629
                     /locus_tag="VF182_11220"
     CDS             53276..53629
                     /locus_tag="VF182_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437693.1"
                     /translation="MTHNLERTMSDDTLTEEFFAAYRRGEHLEPEKHLLMAILQDAIH
                     DYRKHRQVRSPEGKAYFREAEQWITAVDGSWIFSFTNICEILGLDADYVRRGLSDLDT
                     DRAKQREHRTSRELH"
     gene            complement(53869..57936)
                     /locus_tag="VF182_11225"
     CDS             complement(53869..57936)
                     /locus_tag="VF182_11225"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX6437694.1"
                     /translation="MALANVLHDGVMRARFHAEPRMQATELLLQERTPRDVLVTRPRV
                     EEVRAVANVRDLVLPVLRRFNSPHDTTPAVHLLSNGQYSVMVTTAGSGYSHWRNLAVT
                     RWREDRTRDASGTYFFLRDKSSGHVWSAAYQPSGVEPDNYEVTFSEDRVEIRRRDGAI
                     GTTFEIVVSPEHDAEVRRVSITNFGARTREIELTSYAEIVLATPAADSAHPAFSNLFV
                     QTEYVADLGALLATRRPRSHGERQVWAVHVIVAEGETVGAMEYETDRGRFLGRGRGIR
                     TPMSVIDGHPLSNTAGAVLDPITSLRRCVRLAPGASARVTFSTLIAQSRDEALALADE
                     YNNPATFERAATLAWTQAQVQLRHLGIDVDEAYLFQELASRILYSDATLRPSSAVLSG
                     NNRGPSGLWRYGISGDLPIVLIRIDEAEDQSIVRQLLRAHEYWRMKQLAVDLVILNEQ
                     ASSYAQILQEALETQVRASQSRLQHEGQESFGGVFILRGDLISAEERTLVQTAARAVL
                     LSRRGTLAEQVERQQRSQAPALPLPRRPPATKAPDRLPAPPDLEFFNGLGGFTADGRE
                     YVTTLGEGQWTPAPWINVIANPSFGFQVSESGSGYTWSLNSRENQLTPWSNDPVSDPP
                     GETIYVRDEDSGELWGPTVLPIREEAWPYTARHGQGYSRFEHASHGISLNLLQFVPLA
                     DPIKISRLTIENNSGRSRRLSVTAYVEWVLGVSRSGSAPFVVTEIDEETGAMLARNAW
                     NSEFAGRVAFADLSGRQTAWTGDRTEFLGRNGTPDHPGSLERGDSLSGKVGAGLDPCA
                     ALQAMIELRAGDRAEIVFVLGETATTEDARTLIALYRGADLNGTLRAVTKHWDNVLGT
                     VQVTTPDRAMDLMLNRWLLYQTLACRVWSRAGFYQAGGAYGFRDQLQDVMALMVAQRD
                     IARAQLLRAAARQFVEGDVQHWWHPPSGRGVRTRVSDDLLWLPYVVIQYLEVTDDIGV
                     LDEIVPFLDGPVLVAGQEEAYFEPKVSAQTGTLFEHCARALDRSLAAGSHGLPLIGTG
                     DWNDGMNRVGSAGKGESVWLGWFLHTTLWEFAKLADARGEHQRAETWRLRVSALKASL
                     EREAWDGEWYRRAYFDDGTPLGSAGNAECRIDSIAQSWGVISGAAEPARAARAMAAVE
                     KYLVRRAEGLVLLFSPPFDHASPDPGYIKGYLPGIRENGGQYTHAAIWSVIAFAALGD
                     GDKAGELFALLNPINHGSTRADVYRYKVEPYVVAADIYAEPPHVGRGGWTWYTGSAGW
                     MYRAGIEWILGVRLRGARLHFDPCIPRAWRGFEITFRYHSSRYEIVVENPQGVTRGVS
                     SVELDGVSRARGSMHVPLADDGATHHVRVILGAKS"
BASE COUNT        12972 a        15792 c        16311 g        12921 t
ORIGIN      
        1 acgccgctta cttttgcggc ttccattttt ccaaacgctg ctcgagtgct ttcagcagct
       61 cgtttaaagt ggcgccgaga gccgcgatga tgacgatggg aacgaacatc gccgccatct
      121 gaaagcgttg cgccgccact gagctcagat agccgagacc ggtcgccgcg ccgtattgct
      181 cggcgacgac gatgtagacc aaggcgcggc ccagcgccac ccgcatgccc gcgacaatgt
      241 agggaagcga tcccggaacc gacactttga gaaaaatcgt gctgtccttg gcgccgaagg
      301 ctcgcgccat ggtaatcaga cgatgatcgg tgttggcaat accgaccatg gtattgatca
      361 ggatcggaaa cacggccgcc agaattgtca tgatgatttt cgatatcggc cccaggccga
      421 aaaccatgat gatcaatggc aatagggcga tcagcggtgt ggcgtagatc ccggagagca
      481 gcggatcaaa agccttgctg aggctccgat accaacccat cggaactcca atgacaaccc
      541 caagtagcac cgagatcaga aagccgatcg agaaattggt caggctgaag cgcatgtgct
      601 cccagatcga gccgttggca aaaagctcgt agcccttggc gaggatctgc gaaaacggcg
      661 gaaaataaaa tggattgaga atgtgcgtgc gggttgcgat ttcccaggct agaaaaaata
      721 ttataatgcc gataatccca agccagttgt atgcaacctc acgcaagcgc gccagccaca
      781 ggttggagct gggcggatgc gccgcatcga tgggcaccag tttcagtgac tcttgttcct
      841 gcttggacgt agccggctcc cttgttcgtg gtcaccgaat ctattgtcat gggtaggcag
      901 tgtcaagcat tcagcgcatt cgatcgctga gcggtcatcg tccgcggccg ccgtgatcaa
      961 cctgagcccg agcgtcctga agaccccgtt ttttgtcggc ttatcctccg aagcaacacc
     1021 ggccaaatct cgattatcta agtcaggttc gatggatctt acgatgaatc ccgttgcgat
     1081 gtacacgtca gcgaaaacag agagcctcgc cttgaaagaa agggagagat cgattcgaac
     1141 tgggcatggt tacaagactg acatcccggg cgctgagaaa atacggtcat ctaaggatgg
     1201 attgtcttga gccatcgctg gctgatgtac ctattcggtt tcgcccgact cgcaggatct
     1261 tttgagcggt cgagggctaa gtcacaccgg gtttacgaat cgacctatag tgaacggtgg
     1321 aggaggagcg cgctcggcgg agagtgagat gatcgatcga ttaacggcag aaatgacagc
     1381 tctcggatta cgatcgccgc aagggtttta aatccgaggg taaaatgcgc attactgtca
     1441 acggtgaggc acaagtagaa cggcattggc cgctgaacgt ccaatgccgt tgagtaaagg
     1501 ctgaccgcac gcggtcgcca tcctgccagt agagaagttg ctcaagcgcg atgccgggtt
     1561 gcctcgatcg ctccttcctt ccagatgcta tgcttgcgcg tctcaggcat aacgaaaatc
     1621 gcaatcagaa acatgagtaa tggcaaccct atcggatagg caagcgccca agccagactg
     1681 ccggtcgaca gatacaccga ggtggtgatg atgggcacca agccgccgcc ccagccatta
     1741 ccgatgtgat acggcaccga caccgacgtg taacgaatat taccggggaa gaactccgcc
     1801 aagaacgcgg cgatcggacc ataggtcatg ccgacgtagt taactagaat taccacgatg
     1861 aggatcgaca tcggataatt catattgccg gcctcagcgt accttccaag tgctgtgtat
     1921 aatggataat acgtaagtgc cgccaggagc atcccgccca agataatagg ctttctgccg
     1981 atcttgtctg agagccagcc ccagaagatc aaggtgggag tggctatgag cagggcagca
     2041 cccacgatat aactggacgt cagcacgtcg agtttcttga cagtttgcaa gaaatagagc
     2101 gcccagaact gaccgctgta ccatacgcat ccctgcccca gcacgaccac gctggcgatc
     2161 agcacgtaac gcaggttccg gctcatgaac gcctcgcgcc atggatttat cgatgtttgg
     2221 cccttggctt tgatatcctg aaaaatcgga gtctcctgca gagaaaggcg aatgtagata
     2281 gcgatcgcga ccagcagaag cgaaacgata aatgggatac gccagcccca atcgttaaaa
     2341 gcggcttctc cgaggtaagt gcgtgtgccg atgatcacgg ctagcgaaac gacgataccg
     2401 atcgtcggcg aagtctggag ccatccggtg tagtatcccc gcttctcatc cgagacatgc
     2461 tccgcgacat aagtgatagc gccaccatat tctccaccta ggcacagccc ttggatcagg
     2521 cgcaggaaga atatgatgat ggctgccgct atgccaatct gggcataggt gggaatcatg
     2581 ccaatgatcg cagtggagat tcccatcccc gtcaaggtca cgatgaaagt gtatttgcga
     2641 ccaactttgt cgcccagcca gccgaacagg aacgcaccca ggggccggat gagaaagcct
     2701 accgagaaga gcgcgaccgt gctaaggaac gccgcgaccg ggtggccttt ctcgaagaac
     2761 ttgactgcaa ggattgaggc caagctcccg aagatataga agtcatacca ttcgatgata
     2821 tttcccacgg ccgcggctat gacgacgcgg cggagttctt taatcgaaat gtttggtccg
     2881 tccataatgt gtccccctta attcgccgac tcgcgtcagt tctgattgtt catactcgtc
     2941 tcagaactcg aatatgccgt ggcatctcac gccgtagact tcgttcatga gagattgcaa
     3001 aacttaataa gcgatcggtg agttgggtga cgatggcggt cccagcgcaa cggagggcga
     3061 gaactgcagt gccggtacga ctttgtttct tccgccgtgc gatcagccat atgcacctcc
     3121 cgatgcaatt tgagtctgac actaagagcg aaactgccgt ccgaagctat gctctcgatg
     3181 gcgcataaaa gataatgcag gcgccagccg agagttgggt tatgtcccag gacacggacc
     3241 atagaattcc cgttgcaatc ctgagacgat cttctagttg ccctttagca aggggccccc
     3301 aagctgtcaa gcggattctc aattcgggtt gacggctttt ttcgacctga taggctagct
     3361 gaaatcctgg aggtcaccat tgattattaa aaatccgcgc tacgattaca gtccgattat
     3421 cactcgagaa aaaattgcct ggcccgacaa tggccgcatc gccctatggg tcgcgccaaa
     3481 tattgaatac tttcatttcg acatgccgat ccgcggctca ggcagcactc ataagccgga
     3541 cgtgccggga tatactctgc gggactatgg ctcgcgggtg ggcgtgttcc ggatcatgag
     3601 tgtgctcgat aaatttggaa ttcgcgccag cgtcctgctc aatgccgagg tttgcgaaca
     3661 ccatcccgag atcatcaccg aaggcaacaa gcgcaactgg gaatggctcg ggcacggtct
     3721 gaccaacagc gtatcgatgc tggattactc gcctgacgaa gagcgcggaa tcatccgcaa
     3781 ggttaaagag actataacgg catcgacggg caaagagccg aaaggctggt tgggtcccgg
     3841 actgggcgag acattcgaca cgcccgatca tctcgccgcc gaaggattcg aatacgtttg
     3901 cgactggggc aacgacgagc agcccacgcc gatgcgggtg aagacaggta gaatgatcgt
     3961 cgtgccctat gagctcggcg taaacgacat ccgggttttc aatcgggaga accacacggc
     4021 tgaacaatat taccgcatgg tgtgcgatca cttcgacacg ctttaccgcg acagcgtttc
     4081 cggcggccgg gtgctttgtc tgccgctgca tccgttcgtc attggcctgc cgtttcgaat
     4141 ttcatatctg gagaaggcgc tcgattacat ttgctctcat gagggcgtct ggcgcaccac
     4201 gagtggagaa atcgccgagt ggtattataa gaactattac aaagatcccg gaaaaccggc
     4261 ttagcatgct tccgccggta tggacgtgat ggcgcgcggt tggctgatcg gcaacctgtt
     4321 cagagttgat ccggagcttt cttggtcata tttgactcgg tctcttcgat tttttccagg
     4381 accttttccg gatcaacatc tttctccaat tccgccagct ccgggtgatc ggatgccgct
     4441 tctattccca cgcgctcggc tatggcggtg acgaggctga tcaaccgggt tacttcgtgt
     4501 tcagccaaaa gactgatgtg caggtcgagg tcggcgcgcc gatcggcctg tgccgccatt
     4561 cgattctgcg tgatcagtac gaaggtcgaa agaaatatcg cttccacaga tgcgaacatt
     4621 gccaaaacta caaaggtggg gtcaaaggtt ggtagcggtg tccatccaag attaacaacg
     4681 atccaaatgc caaaaccaat taagtgtatg tagacaaagg tcatgctgcc ggcaaaccgg
     4741 cttatggcat ccgctattcg gtcttgaacg ccttttgttt tttcctcctg gttgcgccgc
     4801 aagagtaagg cttggatgtt tctttgaacc acatccgcca tatcgccggg ctcgcgggtt
     4861 atgttcttgc tcatcgattc tacttcgata cgtacaatgg ttggtttagc gcgccgtgag
     4921 tgtagtgata ttacaattct aactcctcta agccgtttag ctagcccggt gccgctgctc
     4981 ggcaccatgt aaccggtttg agtgtttgac gggatgaaaa tgttctagcg acgggcgtag
     5041 agtaatgggt caactttaac ttgttgctcg gactgttata tccatcaaaa cagagcatct
     5101 ttactctatc ggataggaga ccgattatga ctgatggtaa agccaagcag tttgccgtgg
     5161 aaaacatcga gcgaaaccgc gaagcgatcg cgcttttgtg tgacaacatc ttctatttcg
     5221 ccgagctcgg catgcaggag ttcgaaacct ccggcttgat gatgcagatc ctcgagaaag
     5281 ccggatttac cattgagcgc aatccatcgg gaatgccgac ggggttcata gccagctacg
     5341 gatcgggtaa gccggtgatt gcgctgcaca cggaattcga tgcgactccg gggtgctcgc
     5401 aggcgcccgg tgtggcggag ccgacgccga ttgtcgatgg cgctccggga cataccgagg
     5461 ggcacaacgt taacggcgcg gtcatgatcg gcgcggcgtt ggcgattaag aaaaccatcg
     5521 atgagttcgg cctcaaaggc acactcaaag ttttcggcgc ccctgcggaa gagcagctag
     5581 tcagccggcc ttatttcgtt cgcgatggct acttcaaaga tgtcgatgtg gcgttgcatg
     5641 atcatatcgg caaagagctt ggtaccgttt acggcttgcg gcagtatgct cttatttccg
     5701 ctgagttcac cttcaaagga cagagcgccc atgccgccac cgcgccgtgg aacgcccgcg
     5761 atgctttgga tgccgcggtg ctgatggaca tcggctggga taagctacga gagcatctgg
     5821 agccaacgca gcgcagtcac cgtgtgatca ccaacggcgg cgatcagccc aatgtcattc
     5881 cgaatcgttc gacgatctgg tggttcttcc gtgaagcaac ggctgagaag acccgggcgc
     5941 tgtttgaaaa agctaagcgt gttgcccaag gcgcggcatt gatgaccgag acctcctacg
     6001 atgtaaatgt cctgagcgcg gtttggccga cccgggccaa tcgaacgctc gcggaggtca
     6061 ttcaagcaaa tatagaagaa atcggcatgc cgcggtggac agaaggcgag cagaagttag
     6121 tccatgaggt tcaaaccaaa gtaaaggcca agccgaccgg aatgcccacc aaagttgcgc
     6181 cgctcaaaga atgtgtgcaa tcggtttcgg ctaacgattc gggcgaagtg tcctgggtgg
     6241 ttccgaccgg tctgattacc ttcccttcga atattcccgg ggtatcgtac catcactggt
     6301 ccgccggcgt gtctttggcc acatcgatcg cgcacaaagg cgcgatcgcg ggagctaaag
     6361 cgatggcggc atcggcactc gatttattgt tggatccgca aatcgttgcc aaagccaaga
     6421 aaactttcaa ggacgaaatc ggcgatgtcg aatatcggcc tttgctgccg gcggaacaga
     6481 aaccgccgct ggatttgaat cgggatttga tggagcgata ccggccgaag atgcgtgagc
     6541 attatctgag agagaggccg gagtttcggt aaaagtcgtt caaccgttca agctcagagc
     6601 tgaaggccaa gaggctcaag cagttcaaaa cgttgaaact gtgcaaaccg gttaggaaaa
     6661 gaccagtgtg cctgctgttg cgggaggtct cagcgcttag cggggaccga tctcggcggt
     6721 tagtgtcact gaggagcgta cctcgatggt gccggcctcc accggcgtcg gtgcggtgga
     6781 tgacagtgcc tctgcttgaa cggtgcgggt ctgcgcaatg attgggcgaa ctcctcgttc
     6841 attttccgtc agcgacagaa ctcgaacgac tttcaatcct aatgcgccgg ccatttcgtc
     6901 ggcctttgac tgcgcctttt gcgctgccat gcgcagcgcc tgaacttgag ctgaatgctc
     6961 atctttcaat gtgaaaacaa ggcgattgat ctgattcgca ccggcttgca tgccagcgtc
     7021 gattagcttg ccgacgcttt ccaaaattcc ggttttgatc cgcaaaacat tggcagccgt
     7081 gtaaccggtt atttccggct tgccaccttg gggatagcga tagtttggat ttaacgtgta
     7141 tccggtggtt ttcacttcgt cacctttgcc gaggagtttt ttgatctcag ccagcacacg
     7201 gccaagcttc tcggcgtttt ctttggctgc ctccggtgcg gtgcgggctt gcgtgagcac
     7261 cccgatctcg atttgcgcct ggtctggctc tgcaatgatg accgcctcgc cctgggcggt
     7321 gaccgacggc ggtctcactc gatttctttc ttgcgcgtgg acactcgacg acagcagaat
     7381 gagactcata atgagtatgg ataggttcat ggcagatcgg tcctttacag atcttagacg
     7441 aggctatcgc ataaacggaa gcgccgccaa caatcactcg ccctgctggg acaatctccg
     7501 gcgagcctga tagccgatgg cattgcggtt cctgatcttt ttaggtttgc gttgagtagc
     7561 ctagcgagca aagtgtacgc cgcgcttgat tgcaaaaatc aggttaaccg taatccgaca
     7621 ttattcgaat tccgcgaggc aagcgagcct tacagccgtg ggcaatgggc cagtgcctta
     7681 aaggccgccg cgcattcccc taaaggtgat gtttggtacg tcggggcctt agagccaact
     7741 gcgtgctcga ctgcccacgg cttgcagtct caccatcctc gctcactgct gacaacctta
     7801 actagtgtcg gatcagggtt aagttaattg gatgtccagt ttgattcctg ttaaaggcta
     7861 gatgtggaat cgaccatcga gcaggcgcat cccgcagcca aacagaagtt cttctacggt
     7921 tggtacatcg tcggcgttgg ttttcttgcc aatgttgcct cggctttttc tctcgccagc
     7981 accatgagcg tgtttctaaa acctctaact gccgatctgg gcgtctcgcg cggggtgttc
     8041 tcgttgctcc gttcgggcga agggctaatt gccgcattcc tggcgccggt gattggcacg
     8101 ttgatggatc gttatggcgg tcgctggttg atggtctttg gagcgatcat cgccagcctc
     8161 ggctatatcg tactcagcca cgtcgataat tttttccaat tcagcatcgt gcgcttgagt
     8221 ctcatcacca tcggcgattg tttcatgggc tatatcgtaa tcaatctggc gattgcccat
     8281 tggttttcgc gcaagcgcgg ccgggcactg gcgctgacca gcatgggcat cggttttgcc
     8341 aaagtgggca tgcctgtgct ggcggcatcg ttgatcgttt ggctcggttg gcgccagaca
     8401 tggctggtgt tcggcattgt tactttggtt ttggtagtcg gtcccgccct gttgatcgtg
     8461 cgccgctcgc ccgaagacat ggggctccat cccgatggcg ctttgtcctc ggctgcgaaa
     8521 gatgagccgg tcacccactc agccaagact ccttcgatca catcaaaggc gatccgcgac
     8581 gatgtcgagt ggacgcgaca agaagcgcta cacactcggg cgttttggct gctcgtggtc
     8641 accttcggca tttcgagcat cggagtgacc ggcttgaatc ttcacgtttt tccctacgtc
     8701 aatgatttgg gtcatccgcc ggttgtcgcc gccaccgtga tgagcgtgat tgcatccatg
     8761 cagcttgcct cgccgatagc ctggggcatt ctcgccgaac gaatcgacac gcgcttcgcg
     8821 gcgaccttgc gtttcgtcgt ccagggaatc ggtctcggtc tggcggtcat tacggacaat
     8881 ctcttttgtc tctatgccgg gtttcttctt tacggcatcg gtctcggggg caacatggtt
     8941 cttcccgata ttctttgggc agactacttc ggccgtcgtt cactcggaaa agttcgcggc
     9001 ctgggtttgc tcatttcaca gactgtggcc gctttgggcc cgcccttctt cgggttcctg
     9061 ttcgatctca ccggcgccta cgacctctct tttatgcttt tcggcgcggc gctgataacc
     9121 tcggcgtttc tcagcttgat gttgcggccg ccggtgaaaa cgtgcgaaca agcaaactca
     9181 taaactgacg ggtaaggagc attcccatgt cgttgcaaac ggttcatctg gatttcgccg
     9241 agttgactgc agatcagcag gtaggtcgcc ttaaagagca acacgcttta ctgcgcggta
     9301 aaggcgctgt agtgcgggcg cgcgtacatg atctgcctgt gcggcagtac atcgctctgc
     9361 tggagcgcgg ctaccgggtc gggttggagc aagagggtag cggctttacc ttgactctcg
     9421 atccaaatgg atcgacgccg cggacgggac ttcgaggcgc gcactcggtg gtgagccatg
     9481 acgatggccg gatctatgca aatacgactg acaatcgggt cgcggtcatc gatggcggca
     9541 cgcggcgttt gaaaaagcac attcctaccg gcgacgatcc atcccatctg gaattgtccg
     9601 ccgatgggaa acgactttac gtcgccaata gcggctccaa cgacgtgacg atcatcgaca
     9661 ccactgcgga tcaagtgccg gcgacggttt cgaccggaaa gcgtccactg cttccctgca
     9721 tcgctccgaa cggcaacgcc gtttacttgc cgagcggacc cgaacggacg gtcactgtgc
     9781 tcggcgcgga tggcgagttt cgcgcaagga ttgcggttgg catcgcgccg catgacattg
     9841 cggtttctcc ggacagcagt tgggcttatc agcccaactc cgcttcgcat acggtcacga
     9901 tcatcgatgg ccggaactat tcggttgcag gcgagattaa agttggtctc ggcccggggc
     9961 acatcgcctt cgatcccgag aatcgcttcg cctatgtggc gaacaccgtg tccgatgacg
    10021 tgacggtcat cgacgtggga actcatgagg tcgtcgcatc gataccggcc gggcgaggcg
    10081 cgcatcttcc ggtgattggt ccggatggcc gttacggata tgtcgccaat tttgcatcgg
    10141 ataatctgac ggtgtgggat tgtaacaacc atcaagtcat agccaccatt ccagtaggca
    10201 tctatccgca cttcttcgcg ctaagcacgg acggggattg gcttgtggtc tcgaacacgg
    10261 gcgaatccag cgtttgcatc atcgatgctc gccggcacga aatacgagca cgactgcaag
    10321 tcgggccagc gccggcgcac ctggctttca gcccggataa cgaatgcgcg ttcgtcggtt
    10381 gcgagagcag cgatgaagtc gtggttatcg acttaagaaa gcaaacgctc gtggaatcgg
    10441 ttaaggcagg gcagcgcgga ccggaggcac cttggagtta gcatgaattt aaagtagcag
    10501 agctgaaatt ctcttgggcc tatttcacgc tgcgcgcggg tgttggttca tttaggaaag
    10561 atctctcact ttgttcgaga tgacagatcc cgtattttgg tgtgattccg agcggatgcg
    10621 agcaattttt cctgtgattg acttacgaaa tctcaaactg agtactaacc gcgcgctcgg
    10681 tgttggtatt actggacctg tattatcggt ccgattccgc caccaagagt ggaacttatg
    10741 taaatctcac cggagctgga atatgtctcg ccaaatcttt tggtatttgg agaactcttt
    10801 gatatcggcg acgccgatag gatagatttt ctccgtcctg gttttattca cacgttcgac
    10861 cttcgggtgc gctggggttc gcccagcgat cgccatcatt ttttgtccct cctggctgga
    10921 tatccaacgg atgtacaaca atgctgtgtt tgggtgcggt gcgtttttga atagcgctgt
    10981 tgcgccaatc cccgcaacgc cttctgtcag catataattc agcggagatc ctttttttat
    11041 gcgtgcggga aagtgctgtg aaaagcacgt aaagcaagct gcggcttgcc cggagctcag
    11101 aagttccgcc aggttggaat gacctctgtg aaactcggga ttattggcgg caattttctt
    11161 catcaaagca attgctttgt catcgttttt gaatttgtat ttggccaaac caatcagcac
    11221 ttcctcgtcg tttggctcgc caattaatcg gtttttccat ttggggtcgg caaagtcgtc
    11281 gaattccttt ggttcctcct cttttttgac cagattggta ttccaggctg caatgtaaaa
    11341 atccatgtcg gacgctatcc agtagcttcc cttcaagccg tcgggatatt ctttggcctc
    11401 gggaacagcg aactccgtca aaagattttg ctcatacgca tgaactgtat cgtccaagtt
    11461 gatttgcaat acgtcgccta tcgttttacc cccgcgggct tcggcgaccg cgcgcgcaac
    11521 cattttgttg gaagtagcat ccacatgatt gatcttgata ccagggaagc gcttttcgaa
    11581 cgccgggaga accttgtccg cgctgatctg cgccaacgtc ccatagaaat ttaatgtgcc
    11641 gccttccttc aatgccttgt tgtaaagctc gtccatactt gctgactgtg cccaagccga
    11701 cgaagcaaaa agaataaagg cgagtgtgaa gaggctccgt tggatgaatt tcccgatgat
    11761 gctcattgaa aggtcctcct aaatgtcgct gccccagtaa tcctagtttt ttcttgctgg
    11821 ggcgaaacgt tcgaggtctt gatacaaagt ccccgaagaa gatcatcgct agacacgatt
    11881 cttatttcag tgtgcacggg gggtaatatt ttcgcttaac ccgccgggcg cacccggcag
    11941 agataagagc ggtgcggcag cgagccgctg accgggtcag tgatgtccgt gccgatgaga
    12001 gtggatggat tggcgccgtt gctgttgtag ggatcatagc cgggcagttc gagctcttta
    12061 cagtcctgcc accagccgtg ctggcaacag atcacgccgg gaacgatgtt cgtggtcact
    12121 cgtgccttga ctttgatcgc gccacgcggg ctttcgacaa tcatccactc cttgttgtta
    12181 attccataaa gcccggccgt tttaggatga atatcggcgg tggggtgcgg cgagatttta
    12241 cgcaaactcg gcaatgcgcg ttgctggcta tgtacgaagg tcgtgaattt tgcgttggtc
    12301 agaacgagcg gatattccgc ggcgatgtcc gggcgactga tcggactcaa ggcaggctcg
    12361 atgtattccg gcatcgccgg atagccgtgc gcggcaaagg tttcgcagta aagctcaacc
    12421 ttctttgacg gcgtggcgaa accacgcacc gcgccgtctt tgttggtgcc ggcgtatttt
    12481 tgataggctg gctgcgccgg cagtgaaatt ccgccgggtg cctgcttgag ctcggccaaa
    12541 gtcactccgg tgggttcgag ctcataggca taacccgctt cgatatcgcc attccaaaat
    12601 ttttcgccga agcccaaccg tttggccaat tcgaaaatga tccaggtatc cgagcgccgc
    12661 tcgccagccg ctttcactac ggcgggccgg tactgcatgt gagtcttgcc cgccgggcga
    12721 tgttcgaagc tcgtggtaat acttgccatc tcgaggaagc tcgtggccgg cagcacgtaa
    12781 tcgcaaagct cggctgtcgg cgtcataaag agatcggccg ccacgccaag ctccagggcc
    12841 accatcgcct cgcgcgcgcg gctggagtcc gccgtggaca ggaccgtgtt ggagccgaag
    12901 ttcagaaacg ctttgaccgg ataaggttgc tgctcgagaa tcgcggtgaa gatatcgtat
    12961 gccgtgcagt tgccgggttt ggccggcggt ccgagcggtt ttctttcgcg tccgatgcga
    13021 atggccgccg tttccttcgc caaaaattcc ttgccgccga catcattgac tcgcaagcct
    13081 ggaaaactag cgttaccgcc cggccgatca tagtcgccaa gaagcgcata gagcgatgcg
    13141 atggcgcggc tggtttgggt cgcattggta tgttgcccaa gaccgttcca catgtacatg
    13201 ctgaccggtc gattgtgcgc cagtagcaaa gcagtctgcc atacgttttt cgccggcacc
    13261 caggtgatct tttccgagcg ctcgggagca tacgccgagg caatctcgcc gagtttttca
    13321 aaaaccggtt tgcatgggat ttcttcgccg gattttcctc tcaatgcacg aggcccgaac
    13381 agcgcggcct gaatattgtc gcgttcatag ccgccggtgc tcggatcata gatgacggct
    13441 tgattcttta tctcatccca tacgaggtat cgattccgcg cgccgtcgac gaccaggtcg
    13501 gcttcacgga ccaactggtc ggtcgcagta tttagaagaa acgaaccatt ggtccattgc
    13561 cgcgcaaagc ccgcgtcata ccagctctcc tcgacgaggc aatggattag cgccaatgcg
    13621 agcgcgccat cagtgcccgg ccgcggttgc aacagcacgt cggcttgact gccaatgccg
    13681 acacggcgtg gatcgatcac cacggttttc atgccgcggc tgcgcgcagc aacgatatcg
    13741 tgggcgagga tcaatgatgt ggagcttgga ttgtgacccc ataaaagaaa gcttttgctg
    13801 tgtgccaggt ccggggttgg aatgctgctg ccgaaggtgt aactgaaacc ggtgtccttg
    13861 tgccagttgc agacgtgggt cgtcgaaacc cagttcggac tgccgaacaa atacagaaaa
    13921 cggccgagcc agcgctcgac atcagccacc gaagtgccgc tccgggtccc tttaccgagg
    13981 gccaatgctt tagccccatg ctgatcgcgg attgcgctga ggcgctgcgc gatatcatcg
    14041 agcgcgtcct cccagctcac tcgctgccag cctggatcgg cagcgccctt gggtcgggtg
    14101 cgcttgagcg gataatcgag ccggtcggga tggtaaacca attcgggcgc ggccttgccc
    14161 ttaacgcaga taaccccgcc gttgggatga tcatgatcgg cctcaagagc gatgacacgg
    14221 ccgttctcca ctgtagctat ggtggcgcaa tgcgccgtgc atagcgcgca gtacccgcgc
    14281 actgcgcgag tgctgtttgt ttcttccgag accgagaccc tctcactcat ggcttgcggc
    14341 ttactatagc ccattggcct tgtcaactaa gcatctcgac ttgtcagttg tctgattcgc
    14401 agcatctcct cacttcggat tacaccactc gagttcttcg ttgtgatctc tggtcaattc
    14461 ctccgagcgc cgcaaatctc cagcgccacc agcgcataca tctttgcggc atcgacgata
    14521 tcctttatct cgacaaatcg ctctacggcg tccggcgcgc tcttgcgcgg cataccgaag
    14581 gtcgccgcgg ggattcccac ttcattaaaa atgttgatgt cgcgccacat gctggtttca
    14641 ggcgtgctta cgggcggcgg cgcggcgcct ctgacgagta aatgcgcatt gcggaccgca
    14701 tcgattagag cctcgacatt cttcccttca tagcctttgc gcgccatgaa acattcgacc
    14761 gttccgccca gtccgaccga ttgaacaacg tcgcgcagct cacgctcgac ttgattgaat
    14821 tccagcggcg gcggcacgcg tacgtcaaca tagatactac aagttttcgg cgttgtgctg
    14881 ggcttgtagg gagcgccggc gcgaatcgcc ccgatgctga cttttggcac cattttgccg
    14941 accggatatt caatcgtgta cttgctctcg tattccacag cccatttctc gatggcttcg
    15001 attacggcga tcattttgat cagcgcattg ggattatctt tgaactcgcc gcgttcgggt
    15061 aaccgtgggg tataaaaact tctgccgtcc acagtcactt taaagtaagc ggcgcctgcc
    15121 tcgatccagg tgacgccaaa atcggtcgtt tcagcaatca aggcgaaatc tccgttgatg
    15181 ccgtgggtta ccgcgtgccg gctgccatag cccttgccga tgaatctcgc gccgcgaaat
    15241 tcatcgacgg gtcccatgcc gatttcgccg accaccatcg tcatcaggat atccccggcc
    15301 aaacgaatcc cgctgttttt gatggctttg gtcgcgatca tgaacgccgc catcggaccg
    15361 cgatcgtttt gcaccgccag accaaaaatt cttttcttct tctgatcgac tgatgccgtg
    15421 ggaccgggcg gaggttcttc atccaggcgc atcggcatgc cttgttcgga atccatatgg
    15481 ctgttgaaga tcaagctttt accctgcccc gtgccgcgca gtacgccaat gctattggaa
    15541 cgcccttccg aaaccagttg ctgataggag tcgtcaaaac cgtttgcctg gagccattgg
    15601 agcacgaaat ccgccatcgg ttgctcatag cccgacggag ccttgatatt tcccatcgcc
    15661 acagccaact cgaccaactc ctgttcgtcg attaatttga gaaccgcctg ctgctccgtc
    15721 gttgaaccat ccatctatgc ctccctcatt taatcaatct ccacagctgc tgaaatccgc
    15781 tgcatcagac ataactgccg gctcgagaaa aaccaagtct gatgcggatt gcttttactt
    15841 tgaggaaaag tgcattagtt ctgtgacttg aggcacaaca ccgcgtgaaa gaaattcctg
    15901 cgctcgaact tcgcgacgtt tcatacaaag ccaacggcaa aacaattctc cagggtatca
    15961 actggtcggt tcaatccggc gagcattggg ccattctcgg gcccaatggt tccggcaaga
    16021 cctcgctatt aaaaattgcc tgcggctata tctggcccaa cgccggcggt gaagttttcc
    16081 ggcgcgggag gttgctgacg aatcttcccg aactgcgcaa gagcatcggc tgggtaacgt
    16141 cgactttgac gacgcagata ccgaagcacg aaaaagtgat tagaaccgtg gtatcgggaa
    16201 aattcgcaca gatcggcctt ctcgaacagt ttggcggcaa tgccggcaag agggactacg
    16261 ccactgcccg acgttacctg tctgaaatgg gttgcgatca tctgcgcgag caggagtttg
    16321 gcgttctatc ccaaggtgag cagcagaaga tactgatcgc gcgcgctcgc atgacccggc
    16381 cttacctgat tgtgttggat gagccgtgtg ccggcctgga tcccggcgcg cgggaaaact
    16441 tcttggcgac gcttggccga atcgccaggc aggcaaaaat cccggcgctg atctatgtca
    16501 cgcatcacat cgaagagatc ctgccgatct tcagaaaaac tctgatcttg aaagatggcc
    16561 gggtggtgca tagcggtaac acggatcgcg tgctgaaggg cgatgttttg caggatctct
    16621 atggcgtagg cctgagcatc gtgaaaaaga aacggcgttg ctggccggtt gtccgatgat
    16681 cgcagtgcca tacgccttaa tccaactcaa aagagaatgg gagcgatttt atcgccggcc
    16741 gacgctggag aggaaagtcg tggcggcaaa tagctccagc gcgtttcgtg cgaagctgcc
    16801 agtctcggcc gtcagttgca aactagtctg gctccgaatg ttgagcggaa acggattgct
    16861 tgaagggatt aacgggttga gtcgcactac gcgatcaatc atccggcacg ccttggataa
    16921 ggtcacattc ttcacaagat gatttcgccc cagagttacg aaccgaacac acagcggcat
    16981 cgttggtggt cgttagccgt tgtgtcctta gggacgttta tggtgaccac cgatatcggc
    17041 ctgctcagca tatcgcttcc ggtcattatc accgacttac acgccgatct ggctttggct
    17101 ggttggatcg cgttgattta tgcgctggtg acggcatctc tctatcttcc ctgcggcaga
    17161 ctctcggaca tgattggccg ggccaaagtt taccgggttg gattctttct ctatgcagtg
    17221 acttcgctga tcgccggttt tgcgcaggat gcttggcagt tgattttgtt tcgcgggctt
    17281 caggccatcg gcagcgcact gatcatgacc aacagcttcg ccatggtggc ggcaattttc
    17341 ccgcccgaag aacgcggtcg tgccatggga atcgccggcg gcacggtttc agcgctgggt
    17401 tataccttgg gtccggtgat cggcggggtg ctcacttatg cgctcggctg gcgatcgaac
    17461 ttttttctga cctcgtcgct ggcgttcatc ggctttctgg cggcgtgtta tctactgcgc
    17521 gatgacgccg atgcgtcggc acggcagggc gtgaaggagt ccttcgactt tgccggtgcg
    17581 gtgagtttcg cattggctat ctgcgccttg ctgctgggcc taacggcagg gcagagggcg
    17641 ggttggagca gcgttttgat cctaagcgaa ctcggttttg gtctggccac tttaattttt
    17701 ttcatctggt gggaggccgg caaccgcttt cctctgctcg atttgcggct ttttcagatt
    17761 cgcgcgttca ccttcggcaa catcgctcgc ctgctcagct tcatggtcat tagtctaaac
    17821 aatttggcga tgcctttctt tttgcagctc gccatgggct tggatccgct gcacgcgggg
    17881 ctgctggtcg cgccgacgcc tctagcgttg gcgttgctgg cgccgctcgc gggatggttt
    17941 tcggaaaagg tttcggctcg cttgctgacc gcagtcggac taacgctcaa ggcaattgcc
    18001 tgcgttctcc tggcgtatgt ttcggtgacc gcgtcttctt ttgacttggt tctaagatta
    18061 ggacttttag gattggggct gggaattttt caaactccta acaacaattc gctgatgagc
    18121 tcgattccca gggaacggct gggagtggga tcatcttttc tgtcagtagt ccgtagcctg
    18181 ggtcattcgg ccgggacggc cttggcaact gcaattatca gcgcgcggct gctcgcggtt
    18241 accggccaag aggccatcga tgatctgaag aacaccgcaa gcatcggcgc cggtgcggtg
    18301 ctgccggcct ttatggaagg gtttcgctac gcttttctga ctgcggctgc gctctgtgtt
    18361 gtcggggcgg tgatcagcgc gctatcggtg gagaatgatc gatcgacagc tagaattcgc
    18421 tgactccgag gcgcccaacg aaaggcagtt tcaacgcggg cttgagaatg tcaaccccaa
    18481 aacttaggcc aagcagattc aattcgatgc cctctttccg cgccaaggtg attccaaaca
    18541 cgcccaatag tgaaagctgg taaccggttc cgctgggggt gcgatcgatc accgagccat
    18601 taatcggata gtccttaccg attgcggtcg cgggaaggtc catttttagt tcgggcactt
    18661 ggcggccgac ataagccata aaagtattgc tgttgggtcc cggccagagg agataatcct
    18721 tggcataggg ataagtcgca gcggcttcga taattttcgg gatcgccccg gcagcgacat
    18781 cgccgcgaat atccagtaga agctcgggtt cgttgccaaa ccagcgtccg tctggaatac
    18841 ccggcgccga aaccaccacg ggtaaattgc ggtatgcgcg ccaaccgata acatgatgga
    18901 ttgtatactg agttgcatca gccggcttgg tggcgatcca cgtatgcacg ccgaaaatgc
    18961 cgcgccagtt aagcgcgcgc gcgccataaa cttgcacgac cgcttcgggc gtggtctccg
    19021 cgggcggcgc aatcccggcg ctcgaacggt ccgcggttcg atagtctcta cccagcggaa
    19081 cctgtcccga gagcaccgtg tagaggggca gacccaaaac aaacaaaagt gaaaaaataa
    19141 tgatcatcgg agagattatc agagcgagag tataaccatg aatataggcc aaagctagcc
    19201 agccgctgaa ttgaattagt gttcgcgaca cgtgttcgtg ttcgttcgga attcctgatg
    19261 cacattttca cggacacgat cacgaaaatt gctccctgcc gctggaggca tttctaagta
    19321 gtctctcatc agagtttgtg gctattcttg tgatcgcttg caagccagga tagatttccc
    19381 aagcatggca gcggagatac ggatcggaca tggcttcgac gtccatcgat tccgccgcgg
    19441 tcgcaaactg atcctgggcg gtgttgagat accccataaa gctgggctga tcggtcactc
    19501 cgacgccgac gtcctgttac acgctttgat gaacgccctg ctcggcgcaa tggcggaagg
    19561 agacatcggt agccacttcc ctgacagcga tgcgcgttac aaaaatatcg caagcgccaa
    19621 actcttggca gaggttcttg cgatcatgcg acgcaagcgc tttaaactaa tcaacgtcga
    19681 tgtcacgctg atggcgcagg agcccaagct cgcgccctat tacgcggcaa tgaaaacaaa
    19741 cgtcgccgga tacttcaaag tcgacgaaaa tagaatcaat atcaaagcca acaccatgga
    19801 aaaactcggt tgggctggaa agggagaagg catggcggcg acggtggtgg ttttactggc
    19861 gcgataatct ctcctaaggg gccaaatggt tgacaagttt tttattctat attaatcaac
    19921 tgctctgatc ttaagtgatt tctaatctgc ccaacctagc gccgcaagcg cgagaacaag
    19981 agatcgaaaa ggtagctcaa gagcttggcc gtctgattca gggcgccgac cctgagcagc
    20041 gcaacgatct taaagagatg gcatttgccc tcatccgcga ggaattcatc cacaaggctg
    20101 aggatcaaga gagccaacca gggtcagctc cggcgccatt caatccgttg ggaacaggag
    20161 ttttgatttt ttgcgtgggc gcgggtctgt catttatttt tggacctgtc ggtctggtcc
    20221 tcatgttggc cggcgtgatc ttcgtcacat ggggagccgt cattagttgg atcaaaggat
    20281 aacagcagta tgccagaaga ggaaaagaaa aagcgcggta tctggtcccg gagagacttt
    20341 tttacacgcc tcggttgggc tggatttggc gtattcggcg gtttgagtct gttgggtttt
    20401 cttcgttccg cgttcccgcg tgtgttgttc cagccgccgt cgaccttcaa agccggctcg
    20461 ccgtcggact atgccatcgg cgaagtcagc gaaaaataca agcaggatca gcgcgtctgg
    20521 atcgttcggg aagataccgg aatctatgcg atcttcgcca aatgcactca tctcggctgc
    20581 accccgcgct ggctcgccac cgaagccaaa ttcaaatgcc cgtgtcacgg cagcggattt
    20641 ttcaaatccg gattgaattt tgagggcccg gcgccgcggc cgttggaccg ttttcaaatt
    20701 actctcggcg acgatggtca actggtggtc gacaaaagca aagtattcaa gatggctccc
    20761 ggcggggaac cggatgagca gcatgccgac agcatattga aagtttaacg gattggaaca
    20821 tgagtctttt agaaaagatc aaaagcgata tcgtggaaag tcacgtctgg aaatcgatct
    20881 tccgtcacgg ctatgatgat acgccgcgca accgcgtgat gatggtgaca tccaacgtct
    20941 ttcttcatct tcatcccgcc aaggtccgcc gccatgctgt cagaatgcgg ttcacctggt
    21001 gcatgggggg gctgactttc ctgatgtttt tgatcaccgc agtgaccgga gtttacctga
    21061 tgttctatta ccggccggtc gccgagtacg cttacgcgga tatgaagtac ttggagaccg
    21121 atatgccctt cggcatgctc atgcgcaaca tgcatcgatg ggccgcgcac gccatgatcg
    21181 ttttcgtgtg gctgcatatg tttcgcgtat tcctgaccgg ctcttacaaa ccgccgcggg
    21241 agttcaattg gatcgtcggt gtgttgctgc ttgttttgac gctgttgctg agctttaccg
    21301 gttacctgct gccctgggac cagcttgcta tctgggccgt gacggtcggt tcgaatatgg
    21361 cgcgtgcgac gccgttattg ggctatgaag gtccgtttgc tgaatttgtc ggcgccaatc
    21421 caatctacga tgctcgagcc ttcttattcg gcggcggcga gatcggtcct cataccttat
    21481 tgcgattcta catcctgcac tgcattttca ttcctctggt tgccagcatc ttgatggcgg
    21541 tgcatttttg gcggattcgc cgcgatggtt tctcgggtcc ttccttataa gcaacttact
    21601 taagaggagt acaatggcag aagccgttcg agagagaaga gttccggttg acgacaagat
    21661 ccatacctgg ccccatctcg tgcgcatgga gtttctggtc gcgctgtttg tcatcgtcgc
    21721 tttgaccctg tgggcgatga tggtcgatgc gccgctcgaa gaagcggcta acccgacccg
    21781 gacgcccaat ccttccaagg ctccgtggta ttttcttggc ttgcaggaga tgctcgtcta
    21841 tttcgatccg tggcatgcag gcgtcgtgct gccgaccttg attattgtcg gcctgatgat
    21901 catcccctat atcgacatca atcccaaggg aaacggctat tattgtttca aggatcgcaa
    21961 gtacgaaatt ctgacgttct ttctggggtt ccacgtgttg tggatggtta ctatcatcat
    22021 cggcacgttt tttcgcgggc cgggatggaa cttattctgg ccctggcaac actgggacgc
    22081 gcacaaagtc gtggcgttga ccaatgtgga cctgccttat cttttcgggt ttcgcgatta
    22141 ctggtggtcg gcgattttcg ggttgatggt gattggagcc tacttcgtct tcggcttgat
    22201 cgccttctac tactgggttc tcaaaataaa aggaagagag tttctcgagc gctggggcat
    22261 ggtgcggttt accctgaccg cggtgatatt cgtcttgatg ctttcgcttc ccgccaaaat
    22321 gtttctgcgg ttagtattca acgtaaaata tattctagtt actccctgga tcaatatttg
    22381 atgttttatg tcgccaagat tctgcaagcg ctagggctgg cagatgtggg ttatgccttg
    22441 ctcacgggta ttatgaagaa ttcatcgatg gctgaagaaa tcgtattggc actgatcggt
    22501 cttggggttt tttctttggg ccgcgcgcta gagcgcaagg tcgcttgagg gagtcgcgat
    22561 ggcggaacga ggaatcgacc cacaggaaga aaagaaatca tacggcagcg tctttttact
    22621 cggcatagcc ttactcgtgg ctttgtcgat ctgggcgttt tgggatgaca atattacccg
    22681 ccggccgtgg aaatcatttc agtcgcagtt tttccggctc gactacaata aagccaaagc
    22741 ggcgtacgag gaggaagaca agaagcttca ggcggatccc gcgtaccagg agctaaacaa
    22801 gaagctagcc gctatccaag gcagcatcga ggacggcgag ctgggggaaa agcttgcaac
    22861 gctggagaag caggaatccg aggcctctgt gcggttcgga gaaatcgatc aggaggtgaa
    22921 attcatcaaa agcgaactgg aagaagcctg gtacgaacac gatcatgcga ttcaacaaaa
    22981 acgagatccc aaatcttacg cggaacatat tcaggagctt gagaaagaga aagcgaaact
    23041 cgacccggag ctacaagccg cgcggctaaa gcgggacaat atcagagacg agatcaagaa
    23101 gatcaattcg agcagcaaag agcttcagac tgaagtcagc aaactaagcg ctgagcgcga
    23161 tcgctggcgg cgggtgatgg aaaacgcgac cttcaagctg ggtccggtgt ctttctataa
    23221 aattcctaag atccaacaaa ctgtaatgga ggaattcgat cgcaatcgct tcaatcagcc
    23281 gattgcgcgg gtcgaccgct gtcaaacttg ccatttggcg atcaaccgcg ccgggttcga
    23341 gaacgagcct catcctttca agacccaccc gcggcgcgaa gttttattgg ccgatagcgc
    23401 ccatcccccg gaaacattcg gttgcaccgg ttgccacgaa ggccagggcg tcatggtcaa
    23461 cggcgtcaag caagcgcatg gtgaagttca tctttgggag tttccgttac tgcgcggcgt
    23521 gaaaacccaa tcgagctgca cgtcatgcca tctcgatgtg caaaagcttc aagatgttcc
    23581 gctcctgacg gaggggcaac gattgtttga acaagtcggc tgcaccggat gtcatctggt
    23641 tcaaggatac gacgatattc caaagatcgc gccgagcctg acaaagatca gcgccaaggt
    23701 cgacccgagc tggatggtgc gatgggtcga gaatccgcat aatttcagac cgcgcacgcg
    23761 catgccgaat tttcaattca agcccgagga agcgctggcg atctcggctt atatttggtc
    23821 gcaatccaaa gaagaggcgg aaccgtggct gcagcagaat ccgcttcctg ccggtattcg
    23881 cgagggcgat agcaatgagg cggcccgcgg caagaagctg gtcgaatcca tcggctgtaa
    23941 gggctgccac ggcttcgccg agggcgagtt cagcacccct ctggggaaaa ccaaagacct
    24001 cgttccaaac ctcaaggata ttgccgccaa gaccgggccg cagtggatgt tccattggat
    24061 caagaatccc aagggattct cacccgagac gaaaatgccg agcctgcggc tcagcgatga
    24121 tgaagcgcgg gcgattacca gctacctgac gacgttaggc acaaagggcg aaaccatcgc
    24181 cggtctcgaa gaaaaacttg ccaatcccca aaacatcaag gacggcgaag ccttggtgcg
    24241 aaagtacggc tgtcccgggt gtcacgatat caaaggcatg gaaaaagagt cccgcatcgg
    24301 cgtcgaactg accacatttg gctccaagac cgtggaagag ttggcgttcg gtaatcgtac
    24361 cgacgttccc cttacttggg acgattggac gtttcataaa ataaaaacac cccgcggcta
    24421 tgcgacggaa cgggttgaac aactgatgcc gcagttcgat ctcgccgacg aggacattaa
    24481 cgcgctgcga gtgctgctgg gcggtttcag agaaagaaaa gtgggcaagc gctatctcgc
    24541 cgatcacggc caacgcgcca cccaagtggt tgagggtcga aggctgatgc agcagtacaa
    24601 ttgtgtcggc tgtcatgaga ttgaaaatcg aggcgggttt gttaggaagt actacggcga
    24661 aaacattgcc gcggctccgc cgcagcttaa tggtgaagga gagaaggttc agtcgaattg
    24721 gctgttcggc tttttgaagg cgccgtttac gctaaggcct tggcttacca taagaatgcc
    24781 gacgtttgga ttctcggacc agcacacgac tgaattgatc aactacttca acgggctttc
    24841 gagagtggag agcccatttg catacttcga tgaaaaaaat attcctcccg atcatttgga
    24901 cgcggctcgt aagctggtgt ccgaggaata tttcaattgt ttcagttgtc acgtgcgcgg
    24961 cggcaagaat ccggaggggc cgcctgaagg atgggcccct gatttggcaa tggcgcgtca
    25021 gcgtctgaat cccaactgga ttatcaagtg gatacaggat ccgcagaaga ttcagccggg
    25081 cacgaagatg ccgtcttttt atccgggagg tccggacgac atcctgggcg gaaaagacga
    25141 caggcagatt gaagcgctac gcgactatgt gatgagctta ggaagaaccg gcggtgccac
    25201 ggccgcaact ccttcgcccg ccgtcgagga gagtcgcgca aaggccgtca agcgctgaat
    25261 acgaaagttt gctattcgtc taattaacga caaacttaac aaggaggata gtatgacgaa
    25321 gtttggatcc ctagttctat cagccgcaat attcgcgctg ccggcgagca gcctggctca
    25381 gtataaagcc ggcgacgtga aggatggcgg ttcggtttcc gggactgtca agttcaaggg
    25441 caccgcgccg gaacccaaga agttggacgt cagtaaggac aaagaggttt gtggtaaaac
    25501 cccaaaggtc gatccgaccc tagtcgtgaa caatggcaac ctcgccaacg cggtggtgac
    25561 cattactgac atcaagaccg gcaagaagat ggacgcgaaa aaagttacgt tggaccagaa
    25621 gggctgcgag tatcaccctc atgtgctcgc attcccggcc gggagcactg tcgaaatttt
    25681 gaatccagat ggcatacttc acaacgtcca ctcctacagc aaagtgaaca gcccgttcaa
    25741 catggcccag ccgaaattca agaagaactt agatgtgaaa atcgacaaac cggaggccat
    25801 tgaagtgaag tgcgacgtcc acggttggat gcacggctgg ttggtagcca cggaatcccc
    25861 ttatgttgcg gtcacggata actcggggaa tttcaagctg accgacgtgc cgcctggctc
    25921 ttataccatg gaagtttggc acgagaagct tggtaagaac acccagaagg tcaccgtcaa
    25981 ggccaaagag gacgcgaagg ttaactttga gctagcagcc aagtaggttt acttagtctc
    26041 aattgttatg aagcggcgct cagaaatgag cgccgcgttt tttgttgcgc tgcatgccgt
    26101 gagccggttt attcaatatc gatctggtct ttaaagtagt tccatgctct ttccagatcc
    26161 accggcggta acgcctctac ctgacgctta gccgattctt cactggtcaa aggaatcggt
    26221 gtgccgattt gcaatgctcc gtagagaatt tctgctgctt cctccaaata gatcgccatc
    26281 atcacagcct ccggtattgt cctgccgacg gttacctggc cattgcctct gagcagaacg
    26341 gccggttgct caccgatcgt gcttgcgaca tcctcgccca attgcggcgt tgaaataaga
    26401 tccgggagat aaaacaccgg gacaccgccg gcaaaaaagc tgccgtgatt gtggaccggc
    26461 tctagctttc gattcgtcac agagaacata ttggctacac gagcgtgaat tcgagtgatc
    26521 gcattgattc ctggcttcat cttcatgatc gaagcgtgca acgctgcctc gaatggcgca
    26581 ggatgatcgc cagcaactac ttttccatca agatccatgg tcagcagctc ggcttccctg
    26641 accagcgcga ggcttatcct cggcgtgatg agaaactgat ctgaatcgat gatgcgggcg
    26701 ctgacgtggc caaatccttg aaccaatttt tgccgtgaga ggatgcgaca ggcgctgacc
    26761 acgtccttct tcaatttctg caggtccgat cccataaagc tcctttaaat ttcgacaata
    26821 ttaacgtata ttacggtgat caaattttta gtaaaagaga gctgccgtga aaataaaagt
    26881 ggaatcgatt tccgatgcca aaggacagga aaccccgtcc cggtttatgt tgggtatccg
    26941 aaccatcgag attcaagccg tggcagatcg gtggtacggc gagcaagcct gttattttcg
    27001 tgttctgggt agcgatgaga actcatatat tctcaaggga ccgttagaag atggttcttg
    27061 ggagctcgta tccttcaccc acaaagactc gcgcggtacg gaactcgaat atgagggaaa
    27121 acgggagctt caataacagc acttggatca ggccgtgacg cagtgccagt cgtccgatgg
    27181 aagtcattcc tgacgtggtc acctccgccc gctcgcaaac acctggtgcg aggcgctcca
    27241 gcaacaactc tataaccttc tcattcctaa aactctgaca aggacccact cgatgagcaa
    27301 gcagcacttg gtggcggtta tcggcgctgg ccccgctggt atttatggca cacgcaagct
    27361 tgcggaggcg gggcaccgcg ttgtgctgct gaatcgtgac atcaagccgg gaggtttggc
    27421 cgaatatggc atctttctcg ataaagagaa gatgaagacg gggctgcgta agcagttcaa
    27481 gaaaatcctg gctgatccga aagtttttta ccttggccac gtaagcgtcg ggcatgatca
    27541 ggctcttaca ctgaacgatc taaaagaaat cggcttaagc gccatcgttg tgaccgcagg
    27601 agcgcagggg accaagaaac tcggtatcac cggggaggac agcatcggcg tctaccatgc
    27661 caaagacgtt gtctaccact acaatttatt gccgcccttt agtcagagga atttcgaaat
    27721 cggccgcaga gttgccatta tcggcatggg aaacgtcatg gtcgacgtcg cgaattggtt
    27781 gctgaattat aaaaagatcg atcaggtggt ggtggtggcg cggcgcggcc cgctcgaaaa
    27841 ggcgtacgat gacagcgaat ttgaggtgat cgaaccgtat ctcgataatc ttgatatgaa
    27901 agaggagatt gaacgcatac ggccgcaact tacagcggtg ggacagaaca tcgatcagat
    27961 cagcgaaaaa tttgtcaaac agggagagcc tgccgaaggg cgcaggttgc aatttcgtta
    28021 tctatgttcg ccgacgaaag tcatcgccga tgatcgcggt ggggttgccg cgcttgaagt
    28081 tgaagagaac gagttaatac ttcaggatgg caaaacggtc gccaaaggaa ccggtaaaca
    28141 aactcagatc aaagtcgatt gcgtcattta cgcaatcggg gatcaagtcg acgcctcgct
    28201 gggattgccg ttttcacgcg gtgttttcgt gactaatccc agaaaagttc tcgccgatcc
    28261 tgatccagcc gcgtaccagc cctatgatcc tgaagccgcg acagtgctcg acggcattta
    28321 tgttgccgga tggtcgcgca acgccagcgt cggtcttgtc ggcgtcgcta agcaggatgc
    28381 ggagagaggc atcaaagtgg ttaatgccta cttgagcgcc aaggacggat tcacacccgg
    28441 cgagctggaa gccaagatcg aagccattct caagatcctc gacaaaaaac gcgtttccgt
    28501 cgtgaccaaa gaagacatcg aaatccttga gagtgttgaa cacgaggaag cccgcaacag
    28561 aaaaacctgg gatttcaaat tcatgaccga tgatcaaatg ctgcaggcga ttcgagagcg
    28621 caaagcgcgc gctgactccc ctcttaaatt ttcatagcct taattaagcc cgccgctgat
    28681 tctcgaccct cgcctggcca ttgccccagt acagctttcg tgctctccct gatgttgagt
    28741 aagaagaccc aaagcagttc aggagtgcca gcgttccttc catctcggga gagaggacga
    28801 agctcatcta cgacgcaatg gacgactata atccggttga tgaagcacag aacggtcata
    28861 gcggcagtaa gtctcgtgag agtgccggcc gctcgggagt tcattatccc tgccccaggc
    28921 tttacgctgc ggcgttatga tcagaagaaa ttgagggcgg ccgaggtggg gtggaggaag
    28981 cttgacctca taaccgcaca gcctgagctt cttcgctcgc gctaacgctg ctagccagct
    29041 ttcgaatgat tcgcgcgttc ggtctgcgat cacccgcttt gaactggttt gtttgatgag
    29101 gatgatgtct tttcttcgtt tcaagtttgt tcccaatcgg ctgaaccact ccattttgca
    29161 gccgcttcaa cgcaatgaaa gggccatgga caagagtacg tttatcttgt cggtctttga
    29221 aactccggcg agcgttaaag cagttcatgt tccatcgctt tcctattttc acgaattcca
    29281 gcgccgtttt tttgcactca caggaaagaa gctcgggatc gatagatgaa atgatcaaag
    29341 ccgagaacag cattaaatcc tcggaaaatt tcgatgattt cacggaagca agagcttcaa
    29401 ctaatgcacg gcgcagcaac aattggaaag taggttcact ggaaaccttt ttgcaatcat
    29461 tgatggcgcg cttaaatcct cataacggcc caaatcggct gaaagaatgg tcggaattat
    29521 cacgttgaca aacgggccgg tatcatctac cgcggcggta atgcgttaag cgccagttaa
    29581 tttgcgaaat gccgggggaa caatctaagt cgcgattttt tttatggaca aacccatcct
    29641 ggcagtgtta tagcctatgc aattttgcgg tttagacctg cgatgatgga caccgaagga
    29701 aatatcaagc gctcggtgat cggtgtgcta ttatgcagcg tgctgttggg cttcgctgcc
    29761 tcatgggttt ggtccgcggc aaccgatgcg ccgaaacccg ctcctgccac ttatgtcggt
    29821 aatgaagttt gtcaggcttg ccacgcgccc cagttcgaaa aattctcgac aactttgatg
    29881 ggcaagatat tcttgcacaa cccgcgcaac gagacggaaa aaagggcgtg cgagaattgc
    29941 catggtcccg gaagcgagca tgtcgcggcc ggcggcggca aaggggtcgg cgggatgatc
    30001 acgttccgca aagactccgg cgaatccgcc gaagtacaaa acgagccttg cctcggctgc
    30061 catcaacggg gcattcagac tttctggaag gcaagcacgc acgcgggccg cggtttaact
    30121 tgtgtcagct gtcacacgct gatggagaag accagcggca agcaactgac caaacaaact
    30181 gaggtaactc catacggggt caatcgcgcc gagatcgaag tttgcggtca gtgccatctc
    30241 cagcgtaaag cccagctcat gcgctcctct catatgccga tgagagaggg gaagatggtc
    30301 tgctccgatt gtcacaatcc gcacggtagt cccaacccag cgcagctcag gcagacgagt
    30361 gtcaatgaaa attgctacac ctgccacgcg gagcgccgcg gtccgttttt gtgggagcat
    30421 ccgcctgttt tggaagggtg taataactgc cacgagccgc atggttccgt caacgatcgt
    30481 ttgctgaaag tcaccgaccc gcggctttgt actcaatgtc atatccatac gcgccatcca
    30541 gctacgccgc ggccgttgaa accgctttcg ccgaccgtgg gtccatcggt ttttgccttc
    30601 aacagaagct gcaccaactg tcacagccag gtgcatggca gtaatcaccc atcggggaaa
    30661 ttttataccc gttaggggac acggagatcg gatatgatat gcaaaaaaac cattttgcat
    30721 ttgagtatcg gcatagcgac tctgttgtta tttgcggcgc gcggcgcggc gcaaatggaa
    30781 gtgggggatt acacgctcag cggtgcagtt gaagccggcg ctatgccgag ccatcgtagc
    30841 gggaacatct cgaagctcga ggagtatcgc gatcttccgg aaacgctggt cgtgccccag
    30901 ctgcaactat ttatcgacag taagcggaaa gacttttatc tcgagtttga ctcgataaag
    30961 accggccggg acgatcagag ctatcgcctg cgcacaggcc gatacgggct cttggatttc
    31021 gagtttgtct gggatcagat cccgcatctc ttcaacgttg ataacgcgcg cacacctttc
    31081 aacatgaaag acggcagata caccttggca aacaaaccag cgggcacgaa cggcgccgac
    31141 gttcgcgatt gggtcaacgc caacgcagaa cctgtggatc tcaaattgta taacggcctg
    31201 gcccgattca acgtaaggta tacgccaacc ccgggctgga ccttcaacgg cagctacggc
    31261 tcgcaaaata ccaacggcga gcgcgctttc ggcgtttaca tcggtccgag tccgggcagt
    31321 tacaacattt ccgagctggc ggaaccgatc gattatcaaa ctcacaatat cgagctcggc
    31381 ggcgaatatg cgggcgacgg ttggtcgctc gggcttaaat acaacggctc tctgtttcac
    31441 aacaatacca gcacattgac ctgggataac ccgctgaaca tcagcggcat cggcggcgcc
    31501 tgcgtcgatt cggcggttta taatcaaaac accggaacag gtccgtgccg cggccgtgtc
    31561 gatttatatc ccagcaacca agcgcacaac atcaccctga gcggcaccgc gagcttgccg
    31621 ttcaaaacca acttcatcgg cgctttctca tacggatgga ggttgcagga cgatccgttc
    31681 ctgccgttta ccatcaacag cgcggtcacc caacccacga tcagcaggcg cagcctcgat
    31741 ggtgatgtcc gcccaaccat gatcaacgcc acattcgtca ataatttttt cagagacgtg
    31801 ggactcaaag cggcttaccg atactacgat ttcgacaatc gcagtaaacg agtgctcttg
    31861 ccggacggct ttatcgtcaa tgatcaaggc gtggcgaatg gcacagagct tctttcgttc
    31921 ccgtattctt atgccaaaca gaacatcggc ttggatgctt cttacaatgt aacccgatgg
    31981 ctcacggcaa aactaggcta cggttgggag cgaatgcatc gcgaatacgc gcgcgaactt
    32041 ttgaattcca acgagcacag cttcggcccg accttcgaca tcaagccgag cgcctggact
    32101 ttgttccgga tgtcttacag gaggtcgatc cgtgatgcgc atgattacga tgccggtcgc
    32161 aatgtcgcgg tggaaatagg cgaaaccccc gaggacgtcc gcttgtcacg gctcgaagaa
    32221 ttgcggaagt ttgacgaagc cgcgcgcgac agagatagat ttactttttt ttcccaagtc
    32281 tcgccgctgg aaaatttgac tcttcacggc ggctttgatt tcaccaaaga ccgttatccg
    32341 cgcacggtca tcggtgtgca aagagacatc gattatgcgc catcgctcgg gtttatctat
    32401 gcgccgctcg aatggctgtc tgttttcggt gattacaatt gggagcgctt cgattggcgg
    32461 ctgcaatcga tcgcgcgcaa cacgggtccg ggcggatgtc cggatctgga ggcccgcacc
    32521 tcggaaaatt gtcctgccgc tacttggagg agcagaggca acgacgaaat ccataccgtc
    32581 aagatcggca cggacatgca ggtggtcaag aacatattga acctgcggct gcagtatggc
    32641 ttttccgttg gcagcagcga agtcaactcg agtggcaaca gccccgcttc gggggatacc
    32701 ggcattgttc ccgcaaccga ttatccgacg atcgttaacc gctggcatga atttgtggcg
    32761 ggaatggaat acgtgctcag caaaaatctt tcgctcaagc ttggctatta cttcaatagc
    32821 tacagcagca aggacgctgg tgtagatatc atgagggtct ggatgggcga cgttgaaacg
    32881 ccggcaggcg gaccgctttt taacacctca acgggccggt cgatctttct cggcgatcgc
    32941 ggtaaaggat cgtatcaggc tcacatcgga tttctcggcg cgcgggtcaa attctagaaa
    33001 cagcgggcca gcagtgcatg ccgattcgct gcgttgcacc ccggagaaaa ttccgattgc
    33061 cgtccactgt cctactcaat ctacgaaatg agccgacgtc ttcatgagtg ccgatttggc
    33121 tctctcgggg ttgcctcact tcaactagga gaaggcagtc acagccaaga ccatccgtgt
    33181 ttgggcgggc ggttcggcag gcgagcgcat aatcgttcgc tcggatagaa agcccgtgta
    33241 agaccgtcag cctgtgggta cccttcacag gagtcattct tatactatac gcagtcccga
    33301 tggagagtat ccttgaacct gattggagcg attcttctaa cggccgtcgt tgtgctctgt
    33361 agcgatctga cagctggcgc tgcggaaaca aagcctcagg aagatagctg cgcaacctgc
    33421 catctggcac tcggcataga gaagctgacg cagcctgctg agctctacaa agccgatgtc
    33481 catgcctcca aggcttttgg ttgcgctgcc tgtcacggtg gtgaccccaa cattatgggt
    33541 ctcgaggcga tggaccgcaa gaaaggctat atcggcaagc cagcgccgat tcaggtggtg
    33601 gaaatgtgcg gcaaatgcca ctctgatgcg aacttcatga ggcgatataa tccgtcgctg
    33661 cgcgtcgatc aagtcgccga gtattacacg tcggttcatg gcaagagact ccaggatcaa
    33721 aaagacccga aagtggcgac ttgcgcgagc tgccatacac cgcacgcgat ccgaccgccc
    33781 aacgattctc gttcgtcggt ccacccgacc cgggtcgcta aaacctgcgg ctcttgtcac
    33841 gccaatgcgg aatatatggc gtcctataaa attccaaccg accaacttga gcagtatcag
    33901 cagagcgttc actggaagac gatcatcaac aagagcgatc tggcggcgcc gacttgcaac
    33961 gactgtcatg gcaaccatgg cgcggcgcct cccggagtgt cgtctgttgt gaacgtgtgc
    34021 ggccagtgcc acgcggtcaa tgccgagttg ttcaacaaaa gcccgcatag caaagtcttc
    34081 gcgcaaatgg gcatttccgg ttgcgccacg tgtcatggca atcacgcggt cgtcgaaacc
    34141 agcgacgcga tgttgggcgt cggcgataaa tcggcctgtg cgggctgtca tccccccggt
    34201 tcttcgggcg gcgcgcttgc agccagcatg ctcggttcga tcgatcgcct caaagcgggt
    34261 tatgaaaaag cttttgccgt gctagcaaaa gcggagcacg ccggcatgga ggtgagtcaa
    34321 ccattattcg aactcaacca ggccaagacg acgctcatca aggcccgggc agcgattcat
    34381 ggatttgatc agacacttct cgacaaagaa gttgagccag gcctgcaagc aattgaaaag
    34441 tcttatgcgc ggggtgtccg agcgatgagc gagttgcagt ttagacgcag aggattggtt
    34501 gtctcaacgc tgattattct cgccctagtc atcggcttga ttttcaagat ccgccagatg
    34561 gagagaaaat caaagtaacg gcaggaagag gtaaaagtgg ccgggaacga aagatcgatt
    34621 cctctggata acggacgcag acggtttctt aactggtttt tgggcaccac ggccggggcg
    34681 ttctttattt ccgtgaccta tccgttgagc agatatctga taccgcccgc ggtggaggag
    34741 tctaccgcgc acacggtcac tttgtcgcta aaaccgcaag aagtgaaacc gaatagcggt
    34801 cagatcttcc gcttcggcag ccagccggcg atcttgatca agacgccgaa tgacgagctt
    34861 aaagcattca gcgcggcctg tactcaccta gcgtgcatcg tccagtaccg atccgatatc
    34921 agccatgtgt ggtgtgcatg ccacaatggc catttcgatt tgaacggcaa gaatatcgag
    34981 gggcctccgc cgaaacctct ggaagaatac attgtgaacg tcagaagcga tcagatcgtc
    35041 gtcaataaaa gagcctaaat cgacatggaa atgccaacgc ctaagcacgg caaactttgg
    35101 ctctggttcg atgaaaggat cggcctaagc gatttcgcct atcttgcgaa gaaaaaagag
    35161 ataccgctgc atcgtcatac gttttggtat tatttcggcg gcatgacttt gtttctcttc
    35221 atggtccagg tcatcagcgg catcctgctc ctgctctact atcgtccgag cgccgccgag
    35281 gctttcgaat cgattcagtt tctgatgacc gaggtggaat ttggctggtt ggtgcggtcg
    35341 attcattcct ggtcggcgaa tctgatgatc tttgcgatgt tcattcatct ttttagcgcg
    35401 ctgtttttac gcgcttatag ggagcctcgc gagataacct ggctatcagg cggaggactg
    35461 ctcggcttga tgttggcatt cggttttacc ggatacctct tgccgtggaa tgaactggct
    35521 tttttcgcca cacgcgtcgg caccgaaatt cccggcatcg tgccggtcgt cggtcctttc
    35581 ctgcggcggc tgttgcgcgg cggcgacgat gttaccgggg cgacgctcac tcgtttttac
    35641 ggtattcacg tggcgatctt accggctatc gtcactgtcg tgctcgggct ccatctgttt
    35701 ttggttcaga agcacggcat gagcagaccg ccgagcggag aaaccggcga gccgggcaaa
    35761 agtaccatgc ccttttttcc gaactttttt cttcgcgact tggtaggctg gctctgtgcg
    35821 ctggcagtgc tggcggcact cgcggcctat ttccctgccg aacttggaaa gaaagccgat
    35881 ccttttcagc cggcgccggt agggatcaag cctgaatggt atttcatggc gatgttccag
    35941 acgctaaaac tgctgccggc gcatatcatc ggcattgagg gcgagctgct gggcgttttg
    36001 ggattcggtt tgctcggaat aatattggtt ctgatgccat tccttgaccg ccgccatgcc
    36061 ctaaagggct ggagccgctg gccggcggct tttggtgtcg cgatgatcgt ctatgccatc
    36121 gtgctgacct atttgggata tacaatgagt ccgacgaaat gatcagtccc ggtttgataa
    36181 cgattgaccg cgatggtttc actcgtcatt gacagcacag attgcgcggc gaaccgaatt
    36241 attgggtggt aaccgcgtct aacaagtacg gtgcctatat gtgcgacaaa ggcgaccatg
    36301 ggtgaggtac cgtcgacaga ggcaatgccc actcaaagca caacacgaaa caaaggagaa
    36361 actgatgagg cagaagatca acgtcgcgat agcagggtta gcgatctggg cggggacgat
    36421 tgtggctgcg gttgccggcg acgccgacag cggcaaagcg ctttacgaaa agtcgtgcgt
    36481 cggctgccat ggcccggatg gcaaaggcaa tgagaagatg gccaagttgt tgggcgacaa
    36541 gggactcaac attgttggcg acgagacgaa gaaaaaatcc gacgagcaac tcatgaaagt
    36601 tcttagcgaa ggtacgggaa aaatgccggc ttccaaactc tccagagaag agcaaaagca
    36661 ggtattgagt tacgtgcgct cgctggcgaa ataggcggaa gtgaaatctc aggccgctgc
    36721 cgagtgatgc tcaaagcgtc acctcggtac cagtgcaaag ccgattctca agcggttacg
    36781 agttcaatta aatagatagt tatgcccgct aagacaagaa caccgccgag gatgatgaaa
    36841 agcagaaagc ttgtcggatg cgaccagtgg agagtaatcc cttcaacggc cagtccggcg
    36901 agcaataagt agccggcgat ttttagtttt cgatgcaagc gcgatcgttc ttctgtcatg
    36961 tacccgggcc cttccagaat tttttctgat cgatattcgc cacatcgtga accttagtgt
    37021 ggcaggtcaa gcaagacatg tcgttcgtgc cgagcgcttc ccgcatttcg gtgtgcggcg
    37081 aggttgcttc gaaggatcga ccattggcat ggcagtgtaa acattcgcgg tttttgaacg
    37141 gctcatagag cgcaagtttc gccggcgggg ttttaaagta atagacatag atgtgcctga
    37201 gaccgcgcaa tttgctgttt acatcaccga acatcgtgta atcggtgtga caggtaaagc
    37261 aggcatggtc cttggggata cgattgtttt gaaaatgggc ggcgggaaga tagctgggat
    37321 catcgataag aaggcttttt ccgtagggct ccatgacgtg gcaagataaa caaaactcag
    37381 tcgaggtaga ctgctccaag tgaatcgacg cgcccagaac cgtcgccaca atggggagaa
    37441 tgaagaaagc aatgaaggcg acaattttgc ctccttgcgc tgccgtaata gaaggccgcg
    37501 tcagtaatac tccgattagg atgatactca gaacgatgac ggtagcgact gcataatctg
    37561 ccaagattag ttccttctgg ttggtatcgg cgcaccgcgg ttaatcaatt ctcgatattc
    37621 attccgtata ccggatgatc gtcgaattat ccagtaaaaa gttgtatgag ctttcgatca
    37681 ctacctagtg ggatcgtaat ctatgatcgg agtaatgtcc ctcggaagaa atgtagcgat
    37741 tccctgcatg cctgcgtgga gattattgac agtcacttgc cttgattgta aatcctcaga
    37801 ataagagaaa aacaaccagg aggactgccg tgaaatatac agtgctaacg accgcaatta
    37861 gcttagcttt agcgtggccg atgaaccaga gcttggcagc cgacgccgac gtgggcaaga
    37921 cagcttacga gaagaactgt gccggttgtc atggggcgga cggaaaaggc aatccggcat
    37981 tggccaaaac tttaggcgaa aaagaactca acatcgtcgg caaagaaacg atgcaaaaat
    38041 cggacgaggt cttattgaag attatcgctg acggcgcggg caagatgccg ccgagcaaga
    38101 agcttaccaa ggaagagcaa aagtctgtcc tgcagtacag tcgatcgctg gctaaatagc
    38161 tctatcgccg gaaccaccgg ggtgccggat aattgacagc ggctctcatc actatctccg
    38221 gcatggccgc ggggtttgag ttccgtcaaa atctgcgccg gggatcaaac gcgcgtcagc
    38281 ttaagtttgc gcgaaccggc gtcagccccc tcctcacgaa cgtgcctgga cttctcaaaa
    38341 cgaaatactt ggaaaccgtg atttgattta ttctcggttc attagctccg ctctctatcg
    38401 taatctccgg aagttcagta gttgctgtgc agttgattca ctactggtct acttcccggg
    38461 ttccgtatcg taagtgtaaa gcatttgtgg cagcttggct tcggcttcaa tgcggcgcgg
    38521 gttgggataa cgcaacgcct gccagcagag ccaaagcaaa ggtaaagctc cgccgataat
    38581 gaacagagcg tcgcccggca ggcgcgccca ttcgagaaca ttaacccagc gcaaatcgag
    38641 aaaagcgaga ctgcgcgcgt accagtagcc gatatccacg gcataataga gctggacaat
    38701 gccgagtggg aaaagattca caaaggccat ccatgccaag ccgatattca gcgaccagaa
    38761 agatatttta gccgcccggt cgctccatct gtccgcacgg gtcaagtaac gcaggcagaa
    38821 gagcaaaagc ccggcggcga gcatgccgta aacgcccatc atcgaggtgt gggcgtgatt
    38881 ggccgtgagc gcggtgccga tttcataata gcttacgact ggaagattga ttaagaatcc
    38941 aaatacgccg gcgccgagga aattccacac acccaccgct acgagaaacc aaacggccca
    39001 acgatggagg tgatagcgct cgacggtctt ttgctctccc gacttgatga aggaccacgc
    39061 ctcgaggctc agcaacaata atggaatgac ttccatcgcg gagaaagtcg cgcccaaagc
    39121 caggtgcgcc accggcgtgc cgctaaaata gagatgatgc atcgtgccga taacaccgcc
    39181 gagggagtaa aggatgatgt ccagatagat gacgcgcagc gccgtggttt ggcgcacgac
    39241 gcccagcaac acgaacatgt aggcgaccat cacagtggta aatagctcga gaaaatcctc
    39301 gacccaaaga tgcaccaccc agaatcgcca gaagtcattg acgacgaaac cttcgcgagg
    39361 gctcgccagc agccctacgg catagaacgc aggaatggca agggcggcat aaaacagcag
    39421 ccacggcata ttgccgaagt ggtgctcgcg caaagttccg cggagaccgc gaaacaaaat
    39481 gaacacccaa aggaaaagac ccacaacgag caggatctgc cagagcctgc caaggtcgag
    39541 gtattcccag ccttgatggc cgaaccaaaa ccacagtccc tcgcccaacc agccgcgcgc
    39601 tcccaaatat tcccctgcga gactgccgaa gacgacgatg gccaccgcga tgagaagcac
    39661 aatcgtaagc ggcccctgac cggagcgctc tcgtcctgca attagcggca caatgaaaat
    39721 gcccgttgca agaaacgacg ccgacaccca aaaaattgcc agctgaacgt gccaggtccg
    39781 catgatgttg aagggcagaa tcgttgagaa atcgatcccg aagaatccct cgggctcagc
    39841 ccggtaatgc gcaatcaagc cgccggtcaa agtttgcaag acaaacagca gcgaagacac
    39901 gaacagaaac cagactatgg ctcgctgtgc cggcgtcacc gccacttgat cgatggaacg
    39961 gaatcgcacc ggttctgttt ccgtttcgct ccagccaagc caatcgtagc ggccaaagaa
    40021 atacaggatc agcccggtcc cgcctaacaa tccgatgatc gaaatcacgc tccaggtgat
    40081 cgcgtcggcg gtgatgaaat ttcctgccag gggctcgggc ggccagttgt tggtatacga
    40141 ataggagtat ccgggacggt cggtagcgga tgtccaagca gtccaggcaa aaaaagccgt
    40201 gagcttgcga atatcatcgg gattgctgat ccattctgcc tgagcgccgc cacgggtttt
    40261 ctttgcgaaa aagatcgact gatagtgctc ctccatcatt ctgtgtgccg ctgctcttgc
    40321 tccggtccaa acaagcgaat cgtccgccgg gttgtaagtg tttttgtgca gctcggcgat
    40381 aactcgttcg cgtgatgaaa gaccgcccat gggcgcgcct gcatacgact tgatgaggaa
    40441 ctcggaagta ttgtgaagat attcggcggt gaagtcaggg ccgagataag cgccgtgccc
    40501 gtacacggaa ccgtattcca ttaggccgta ccgttggaaa acattcattc cggcgaaaat
    40561 atcttcgcgg gtaaacagga ctttgtcttc cgccaggact ctggccggga tcggtggttg
    40621 gtcacgatag accaagtagg cgagcactcc gagtacggta aaaccgaaga gataagtcag
    40681 caccgctccc tgaaaccaac cagacgaaat cggcatcggt tctcgcgcca ttatgtgcca
    40741 cctccctgtt ccgcttcgat taaggctgca tcggaactct ttaagttttg tttggttgac
    40801 ttgccgaacc cggatcgcga gcgcagtctc tggacccagg gcttgcgaca atagaacata
    40861 atgaaccgct tggagtccag tgatctctcg acctcatcgg caccgtgggc cgtagaacac
    40921 agcgccgctg agctctcggc gtgaacggtt gaactctaga aaaggcagcg gtcgcctcca
    40981 ccggtatttt caacgctact cgcgtgttgt cccttcccca tggaggctaa gctcaccgga
    41041 ctcgctctaa tccccgcgtg gccgatggtg atatttctac tggagttaaa aagctaacga
    41101 gccggtcgtg ccaccaacac gtcgcaagag acttcccgga gcacgtgttc agccacactg
    41161 ccgagcaaga catgtggcag tcctgtacgg ccagcgctgc caacagctac gaggtccgga
    41221 cgtaaacgtc gcgcggccga ggagatgacc tggggtgcgc gcccgtatcg taccagccgc
    41281 acgaaagacg tgttgtccaa gccccgctta cgaataaaat gctttagctt ttcctggctc
    41341 tggtttgcca gctcacggcg ataacgcaga atgtccgatt ttcgaaaatc cgatcgccag
    41401 agttgttgct caattccctg atatgcgtga aggagaacgt atttcgcgct aggcgctacc
    41461 cgcatagcaa gcgccagcgc ttctccggat tggtcggaga aatcggtcgc cattaggaca
    41521 gttcggtagg ggccacgaac ccgccgtttg acgaccagta cggggcgatc tcctctgcgc
    41581 actattcttt ccacggttgt accgaaccgc aacttgttga tgaattgctc cccgtgggcg
    41641 cccacgagaa tgatgtcgac tgctttctcc cgtgcccgac gaatgatctc tacaaaggct
    41701 gtgcccgtgg tgacttcaat ggacactgtt ttctcacgat caggggatag ttttatgact
    41761 ttccggtgca gatactgctc cacttgtaga acgatttgtc gcttgctagc ttcatctccc
    41821 agcgcgtccg gcaacacatg aagaatcgta agtgtcgctc tatgttcatc ggccagctgc
    41881 acggcgcgcg ctaccgcctt cactgcgcgc ggtgacaaat cggtcgcgcc aaggattcta
    41941 cttagaagcg atacattatg gggttccctc atggtcgtcc ctccgttgtt gccggcgcaa
    42001 tatcgggaac atcgaggcaa gagctgatag cggtgattgg cgcttgtctc cagtcactct
    42061 cgttagttgt tcctccaagg cgaagcgaaa accaaagaat gacttcggct tgtctcccgg
    42121 gataagttac ggattttgac agttagtagc aaaccaactc acagcatgcc ggcagaaaac
    42181 tttattgttt ctgaagcgtg aattgccgcg cctcattgtc atttccgcaa atcaatctca
    42241 ttttttttgc aattctgaaa atctggctgc gaaagactca tgcaggccaa gaaatccttg
    42301 gcatgccggt tgcgctgtgt ctagatccta agttggcgcg gtaaggagag atagcaatca
    42361 cacagagagc gccgggtcgg cgtagtttcc tgcgcggaaa taggataaga aagaaaaccg
    42421 gtcgtgacaa ggttttgcat gatgccgtga tgctctgtac gtgatcaagc cgactggtga
    42481 acagcatcac gagattcatt ccgtgctctg caaacagatc gaacgtgttc gcaccgaatc
    42541 gtcgccgccc gaaatgatct gaacatcatc aggaagattt tttcagggcc cggaaagatg
    42601 gacaccaacg ctctgtgcgc attctcgttc ttcgtacatt atgggtgatc aggcatttga
    42661 ctgccgtttg gggggacaga tccttatgaa tcagcgaacc attacggtgg acggcaacga
    42721 agcagccgcc tccgttgcgc atcggatgag cgaagtcatc gctatttatc cgattacgcc
    42781 ctcgtctcct atgggtgaat tgtccgatga atggtcgaat caagcgcgtc ggaacctttg
    42841 gggcaacatt ccggaggtta tcgagatgca gtcggaagca ggcgcggcgg gcgcggtgca
    42901 tggcgcgctg caggcgggcg ctctggcgac gaccttcacc gcatcgcaag gtttgctgct
    42961 gatgatcccc aacatgttca agatcgccgg ggagctcacc ccatttacca tgcatgtggc
    43021 ggcgcggacc gtcgccactc atgcgctgtc gatctttggc gaccattccg acattatggc
    43081 gtgccgccca accgggttcg cgatgctcgg cgccgcgtcg gttcaagagg cgcacgactt
    43141 cgcggccatt gcccatgcgg cgacgctcga gtcccgcgtg ccattcctgc attttttcga
    43201 cggctttcga acctcgcacg aagtctccaa gattcatgag ctgagcgatg acgatttacg
    43261 aaccctgatg ccggaagctc cgatcgccgc tcatcgagcg cgtggactga cgcctgaccg
    43321 gcccgtgctg cgcggcacgg cgcaaaatcc ggatacgttt ttccaggcgc gcgaagccac
    43381 aaacctattc tatcgggcct gccctgcgat cgtcgaggaa atcatgacgc gattcgctgc
    43441 gcgcacaggc cgaaggtacg gcttgttcga gtacgtcggc catcctgacg ctgaacgagt
    43501 gctcgtgatc atggggtcag gcgccgagac tgcgcacgag actgtggagt ttctcgcggc
    43561 tcgcggcgag aaggtcggcg tgttgaaggt gcgtctgttt cggccatttg cgatcagcgc
    43621 attcttaagc gtgcttcccc agtcggttcg cgccatcgcg gtcatggacc gcaccaaaga
    43681 accgggagcg ctcggtgagc cgctctatca agatgtagtc accgccctgg cggaggcccg
    43741 cgcggacgga ggctcaccct tcacggtcga gccgcgcgtc gtgggcggtc gctacggctt
    43801 gtcatcaaaa gagttcacgc ccgccatggt gaaggcagtc ttggacaacc tcgcacagcc
    43861 tgacccgaag aaccacttca caatcggcat cgtcgacgat gtgactcata cctcgctgcc
    43921 ctacgatgcg gcattcgaga ccgagccggc ggatatcgcc acggcgctct tctatggcct
    43981 cggtgctgat gggaccgtcg gcgccaacaa aaactcgatc aagatcatcg gcgccgagac
    44041 cgatttgtac gtccagggat acttcgttta tgactcgaag aagtcggggg cgacgacggt
    44101 atcccacctg cgcacgagcc ctcggccgat tcgctccgcg tacctgattc agaaagcgcg
    44161 cttcgtcgcg tgccatcagt tcgaattctt agaccggatc gacgtgcttg aacatgccgc
    44221 cgaccattcc gtgttcctgt tgaacgcgcc gggaggcgtc gacctactct gggagcgcct
    44281 gccacgtgag atgcagcagc agatcatcga caagcacatc cgcttctacg ccatcgatgc
    44341 catcgccgtg gctcgccagg cgggcatggg cggccggctc aataccatca tgcaaacatg
    44401 cttctttgcg atctcaggcg cgctgccgcg cgaagaggcc attgtgcaga tcaaacatgc
    44461 gattgaaaag acctatcaca agcgtggtcc cgaggtggtg aagcgcaact gggaagcagt
    44521 cgatttgacc ttggcgcacc tgcatgaagt atcggtgcct gaggtagtca caggaaccca
    44581 tgcgcgtccg ccgctggtgt cggcgctggc gccagacttt gttcagagag tcacagccgt
    44641 gcttatggcg ggaaagggcg accttttacc ggtcagtgct tttccggtgg atggaacctg
    44701 gccgctgggc acggccaagt gggaaaagcg aaacttggcg ctcgagatcc cggtttggga
    44761 tacgagtatt tgtatccagt gcaaccagtg cgccctggtt tgcccgcatg ccgccattcg
    44821 cgccaaggtt tatgacgccg atgcgctcgc taccgcgccg ccgacgttca agtcggcgcc
    44881 ctataagggc ctggagttca aggggcgaca gtacacgatt caagtggcgc ctgaagactg
    44941 cacgggttgc aatttgtgtg tcacggtctg tcccgcgaag gatcgcacca acccccggca
    45001 taaggcgatc gacatgcatc ctcaagcgcc gctgcgcgat gccgagcgtg acaactatga
    45061 attttttctg aagctgccgg atcccgcgca ctccgacctg cccaaggtgg accataagac
    45121 ctcgcagttt cttgaaccgt tattcgagta ctcgggggca tgcgccgggt gcggcgagac
    45181 cccatatctc aagctgctca cccagctctt tggcgatcgc gtgctgatcg ctaacgcaac
    45241 cggatgctcg tcgatctatg gcggtaacct gccaaccact ccctacacga ccaaccggga
    45301 gggacgggga ccggcttggt cgaattccct attcgaggat aatgccgaat tcggccttgg
    45361 gctccggctc ggggtcgata gtcatagccg cgcggcgcga gcgctcgtgg aacatcagag
    45421 cggccgcatt ggcgacgcac tcgcgggcga gttgctcgag gccgaccagt cgacggaagc
    45481 gggcattcac gcgcagcgtg aacgggtaat cgcgttgcgc caggtcctcg cgcgtctcac
    45541 cacgcccgag gcgcgccgtc tcgacgccct cgccgattac ctggtgaaga agagcgtctg
    45601 gctggtggga ggcgacgggt gggcgtacga tatcggctat ggagggctcg accacatcct
    45661 ggcgagccgg cgcgacgtca acgtgctcgt gctcgacacc gaggtctact cgaacacggg
    45721 cggccagcag tccaaggcga cgccgctcgg cgcggtggct aagttcgcgg cggcgggcaa
    45781 gacggtccaa aaaaaggatc tcggcctgct cgccaacatg tacggccatg tgtatgtggc
    45841 gcgtgtggcc tttggcgcga agatgccgca aaccgtccag gcgtttctcg aagccgaggc
    45901 gtatccgggt ccgtccctta tcattgcata cagccactgc atcgcccacg gctacgatat
    45961 ggcgcaggga gccgcgcagc agaagctggc ggtcaattcc ggggtgtggc cgctcttccg
    46021 cttcgatccc cggcgcatcg cgaagggaga gccgccgctt catctcgatt atggtccgcc
    46081 gaaagcgcag gtggccgaat acatgcggaa cgagtcgcgc ttccgcgtcg tcgaacgcac
    46141 cgactccgtc cgtttcaagg agtttctgaa agagtcgcag gcagcggcgc agcagcgcta
    46201 tgcggtgtac cagcaactcg ccggcatcac cgtgccacag gtcaaagtga cgatcgatga
    46261 ccaggacacg ccggccgccg agaacgagga ttgatatgga cttgagtact cagtatctag
    46321 gcttcacctt gccgcatccg ctcatcgcag gagcttcacc gctttccgac gacttggaca
    46381 gcgtgaagcg tctcgaggac gccggcgcct cagctctggt cctccgatcg ctttttgaag
    46441 agcagattac ccacgagcag atggccgagc attttcattt agatgcccac gctgaatcgt
    46501 tcgccgaggc cactagttac ttcccgggcc ccgacgcgtt tgcgttgggg accgagcagt
    46561 acttggaaca tttgcgacga gtgaaggaat cggtgcgcat tccggtcatg gcatcactca
    46621 atggcacgac cctcggtggc tggctcgaat atgcgcgcct catggctcag gctggcgctg
    46681 atgcactcga actcaacatc tatcgcatcg cgacggatcc cgaaataagc agcggcgata
    46741 tcgaacgaca gacgattgat atcgtgcgac atgtcaaaca ggccgtggcg attccggtgg
    46801 cggtcaagct ctcgccattc ttcacttcga tcgctcatgt ggcccgggag attgaccaga
    46861 ccggtgccga tggactgatc ctgttcaatc gtttttatca accggacatc gacgctgagg
    46921 agttggcggt ctcgcgcact ttacatctat cggattcgtc cgagctcctg ctgcgcttgc
    46981 actggatggc gatcctctcg ggacgtatcc gcgcgtcgct ggcggtgacg ggcggtgtgc
    47041 acacagcact cgatgtagtc aaagccacga tggccggcgc gcacgccact cagatggtct
    47101 ccgcgctgct ggcgcacggg cccattcgca tccgagcggt attcgaagat ctcgtcgcct
    47161 ggctggagga acacgattgg aactcgcttg gcgaaatgcg cggtaacatg aatctcctaa
    47221 aggtgcccga tccggacgcg tacgaacggg cgaactacat gctcatgctc cagagctggc
    47281 gcgtctgatc acgagacgcg caacgaacct ttcgacaccc ccgtgccctt gggtattgta
    47341 ctcacggcta taacaggcgt gatcgttgcc agccatgatg aaagaatgat cggtgttatc
    47401 gatcggatct atcacatgag cgagggccgg atcatcagcc atctgagccg tcagagcggc
    47461 aagcgaaagg agatcaaatg tatgcctgac tggtcgtggt tcattgcggc gtttgtcgtc
    47521 tacattattt cggtgcgatg ggtgctgccg cgtttcggtg tgcccgcgtg agtggcaaat
    47581 tctggcgacg tcgagtctcg acgggacgac aaagaaaaga agctgtagcg cgataatgga
    47641 atcggctcaa ccaataatcc tcgtacctag aaccgtgccg gtgagttgcc gcactcatct
    47701 cccggcctct tggcttggcc ttgatctttc ttcgccagct tgcaaatctc tccgccatcg
    47761 ttggctctgg gagaaaatga gccgcgaact ttcgatcctt tccaaatgac cgcactggta
    47821 ttacgttgcc tagcatccct atatacgaga aggtgggtga ctgaaaccat tgccgtcgag
    47881 ggatgccggg ttaggctatc aagcgcttgc cctgacttgt gtcaaaaaac tgccgctttt
    47941 gacgcatgta gcctttctcg gaaaaaagct gggcgcattg gtatgaagta gcacggcgaa
    48001 tcttgataca aatggatcag ccaagcatta aattcttggg tgccgcaggt accgttaccg
    48061 gatcgaagtt tctcgtctcc tataatggaa ccgagattct gttggattgt ggtttgtttc
    48121 agggattaaa agagctgcgg ctgcgtaact ggtctccatt gccggtaaaa gttcaggctc
    48181 tccgcgccgt catcctgact cacgcccata tcgaccattc cggctatctt cccaagctta
    48241 tcaagctcgg ctttcgcggc gatgtttacg ccacgtccgc aacctgcgat ctgctgaaaa
    48301 ttctcttacc tgattctgct tatctccagg aggaagaagc ccgctatgcc aatcggcgcg
    48361 gctattccaa gcaccgcccc gccctgccgc tttatactgt tgaggatgcc gagcgtgctc
    48421 tcgcgctcct ccggccggta agttacgccg acgagatcga gctcgccaag ggattgtctt
    48481 gccatttttc tcgcgtcggc catatcctcg gtgcggctgc tatccgcatc ggctttgaca
    48541 cagccaatgg aaagagattt ttagtcgatt caggagatct tggtcggtat ggaaggccaa
    48601 tcctaaagga tcctgacccc atcgaacacg ccgattggct gctggtcgag tcgacctatg
    48661 gaaatcgaac gcatccaacc aactcggaag acgagctggc gaaagtcatc aaaaaggtag
    48721 ctgtcgggat gggatgctta atcatcccat cattcgccgt cggtaggacg caggagatta
    48781 tttacagcat ccgtgcacta gaggaccaag gaaaaatacc gccgattccg gtgcacatcg
    48841 acagccccat ggcgatagat gcaacggatg tttactgcgc gcattctgaa gagcacgatc
    48901 tcgatatgaa gctcctgagc gatgcgaagc tctgcccgct ctcttccaag aagttttaca
    48961 tacacagaac tgccgaagaa tccaaagcca tcaacgacct ggccggaccc ctaataatca
    49021 tttcggcaag cggaatggtc accggcggcc gcgttctgca tcatttgaag cgccggctgc
    49081 ccgatgacaa aacaaccgtg ctgctggttg gttttcaggc cgaagggacc cgcggtagaa
    49141 cgcttcagag cggcaagaac gaagtaaaaa tgctgggtga aataattccg gtcagagccg
    49201 aagtgaaaac catcgacggc ttttccgccc atgccgatca agacgagatt ctacggtggc
    49261 taagatcttt caaaaagccg ccgagaagaa ctttcgtcac ccatggcgag ccgccggcta
    49321 gtcaggctct gcacaccgtc ctacgggacg agttgaagtg gaatacggaa atccccagca
    49381 tcggccagac tgttgtcttg caatgatcgt gggctcctgc ggcacggctg gattgaagcg
    49441 gataccttct gcttgcgcga tcgaatcttc aacaagctta tagctatctg atgctttctg
    49501 gattgacccg aatgcgcgcg tcaaccacct gacatccccc gcctggtggt aatgcgatga
    49561 ccgggtttag atccagctct ccgatttccg gaacgtcttc caccaatcgc gccacacgca
    49621 aaagcagatc ttcgatcgca ggtatatccg ccggccgatg gccgcggtag ccctggagca
    49681 ggcgatagcc ccgaatgctt cgaatcatct ccgcggcgtc ccggtcagtg atcggcgtca
    49741 cacgaaagca aacgtctctg agaatctcga cgtgaatacc gccaagacca aaggcaatca
    49801 acgggccgaa agaaggatct tggctgactc cgatcatgag ctcaacaccg cctgaaatca
    49861 tcggctgcac gatcgcccca tccatcgcct tcgacatttc tcgctttgcc aattgatcgc
    49921 taacgtactc gaaggcgcgg cgcactgcat ccccgtcttg tagattcaat tgaacgccgc
    49981 cgaattcagt tttatgaatg atttcgcgcg aggccagttt aagcgcaacg ggaaatccaa
    50041 tcttgcgcgc cgcttgaacc gcttcctccg ctgtgcgaca gaggccgtag ggggtcaaag
    50101 gcaacgccat tgcggtgagg acctggcggg tctcttctgc cgacagccag ccggcgccgc
    50161 gctcggcaag gatttcccgg cacagcgatc gcgccacagc gggttcgatg tcatcaaagt
    50221 ccggtatgat tccaaggggt tgttttttcc attcgctgta attaacgagt ttgctcaaaa
    50281 cgcgcgcggg actttccgga aatggaaagt tagggatgtt ctcgccatca gctttgatct
    50341 gcatccgggt ctctgccgct gataggacgc aggctagaac cggcttttgc gcttcaaccg
    50401 ttttcctcaa aacggcgaca ccggaggcaa tgctcttggc catggaattc gtgtccgcga
    50461 gacctacatc gatgtagagc acgatcaccg catcgagttc tgcgctcggc agaaggatct
    50521 ccaaagcctt ggtgaagtgc tcggtgcccg cggatgcgat catgtcgacg ggattggtta
    50581 cactcgcagc tgcgggcaag aatgctttta actgagtttt agtcctgtca tcgagctcgg
    50641 gaacaatcag gccgttggct tcgcagctat cggcgcaaag gattcctaga ccgcctgcgt
    50701 tggttagaat tccgacccgg cggccgcctg gcaagggttg agtgctgagc gccatcgcca
    50761 ggtcgaacaa ctcctccaac gtatccgcgc gaatgacgcc ggtctggtga aaaagcgaat
    50821 caaccgcgac atcgcttgct gccagggcag cggtatgcga gccagccgcc cggtgtccgg
    50881 cgctggttct tccggccttg acagtgacga taggtttgcg gcgcgcgact cgcttggcga
    50941 tgcgggcgaa gcgccttggg tttccaaatg attccaggta gaggaggatg acttgcgttt
    51001 gcttgtcttc ctcccagtat tgaagcagat cattccccga aacgtcggct ttgttgccga
    51061 cacttacaaa gttcgacagt ccaatctgcc gttcacgcgc caaagcgagc acagcaagac
    51121 ccagcgcgcc gctctgtgat gacaaggcga tgccacctgg cgctgggaag agtggtgaga
    51181 aagtggcatt caaactcacc gccgggtctg cgttcataat tcccagacag ttgggtccga
    51241 tcattcgcat gccatagccg cgaactttcc caacgagctg gtgctgcaaa acaacgccat
    51301 cattgcctgc ttccgcgaat ccggcggtaa tcacgactaa agccctgact tggcgcttgg
    51361 cgcaatcatc aatgacgccg agaaccgcct cgggtggcac ggcaataatc gccagatcaa
    51421 ccggctgcgg tatggcggca acggtcggat aaactgtgag gtcgttgatc gagaccatgt
    51481 gtgggtttac gggataaatt cgtcccgaaa acccggcctc gagcagcgca ttcagaatcc
    51541 ggccgccgat tcttgccgga ttgcgcgaag cgccgaccaa agccaccgag cttggtcgaa
    51601 agaaagcctg cagtgatgca atcgtcgaag ctcggtcgcg cagttcggcg cgtgtgacgc
    51661 taagttcgtt gggactaacc gaaagatcga tctcaacata gtcatctgag tgtttgcggt
    51721 ggcattcgaa gccggactgc aaaaacacat ccagcatcgg ttggttgtcg ctcaaggtga
    51781 gggctcgaaa acggcgaaag ccgcttctca ccgccagcaa tgtcaggcgc tcgaggagca
    51841 atgtaccgat gcctttgcca tgaaatgaat cgtccacggc catcgcaacc tctgccgtgt
    51901 tgtcgtcaag cgcgacgtaa tttcctgtgg cgattatctg ccggccggcg gcgccggttc
    51961 gagtgacaat aagactgagc tgtttgcgta catccgaaga atcgcaaaat gcgttgatca
    52021 acttgtcact aggacggccg aagccaaaga atcgccgcct tctggactct ccagagagtc
    52081 cgatgaagaa gttcatcatg gcctggcaat ctttcggttg agccagccgg atctccgcaa
    52141 cggttccgtc gcgcaggatc aatcgacctg attccgcgga gtcttgatac ggtacaggca
    52201 aatatcgtgg gtgaacggac gccacagagc cttgtagata actaccaggg gagtttcaag
    52261 agtgaacgcg tatcaactac atccgctagc aactgccgga ttaggaggga tattaccggg
    52321 cagtcttgaa cagatcccag cgcagtgtaa aacgtgacga ttgatgcaat aaggaaagcc
    52381 atgggccgcg gagcaccagt ccagcatcgc tcaactaaac tcccgttttt ctatctcgcg
    52441 gctccgcatc agagcttgat cgatgcgcat agtttcattg gcataccagt cagctaagct
    52501 atcgaggaga tgacatctgc tttcgtaggc ggccctgtcg atcgagtgtt tattttcggc
    52561 cagagtccgt tgttctttat tcagatagtc tagcgtcctc aaaatttcgc ctcggcgctc
    52621 gcgtaacctt tcgtaatatt cctcgttttt gtttgtgatc atcatctgat ctccatcggg
    52681 attccggtcc taaagatcat ccgtaaccac tcaaccctca cccactgatc atgtgctacg
    52741 aattgcagca gcgccattgt gagccggtgc gtttaccttc tccgggcctc agataaattg
    52801 ccccagcgcg tgttcttggc cgaattcttt gcgccgctca tcttcctagt tgtagtgaat
    52861 ttgccggcga tgtcagcagg cctccaaccc cgaacctcgc gacaaacatc ccacgggcgg
    52921 tggatcattt catcgaagag gcgttccact tcgctcgaga gatgaatcgg gaatgagact
    52981 aggtcgttgt gtttttttgc caacgccgcc actctcctct aagttcgtac cgtgcgttgg
    53041 agcgagtata ctcaaagcta aacgcaacgg ccgtgccagt taggaactcg atttagttta
    53101 atgtttcaat gaccataaat gcgccatttc ttaatagtgg gaagcgacgg gtgctttctt
    53161 ggcattattg cagacggcgg cctgccggcg cccactaatt tgctggcaca tcggttgcgg
    53221 tttgatccat ctaaatgttt aagtcgcgcg ctcgtaaaga aaggaggtta ggacgatgac
    53281 acacaatctg gaacggacga tgagcgatga cactcttacg gaagagttct ttgctgctta
    53341 tcgccgcggc gagcatctcg aaccggaaaa gcatttgttg atggcgattc tgcaggatgc
    53401 gattcacgat tatcgcaagc atcggcaggt tcgaagtccg gagggaaagg cgtacttccg
    53461 cgaggccgag cagtggatca cggctgtcga tggcagctgg attttctcgt tcaccaatat
    53521 ctgcgagatt ttgggtctcg atgccgatta tgtccgccgc gggctcagcg acttggatac
    53581 ggatcgggcc aagcagcggg aacatcgtac aagccgagaa ctgcattaat cctttgctga
    53641 atcttcaacg cacgagaaat gggtcgataa agtcatcaga tcagtgccac gcaagctaca
    53701 accgattcaa atgcggcggc cagtagagca ataggggccc gcacgcgtgc cgcacgcaag
    53761 atgccgcgat gctacccatt acgattcgct tgccgcgttg cgagcatcct agtgggtgct
    53821 tttgttcgga catttacccg gttagttttc atccggtacg gctcatctct aactcttggc
    53881 cccaaggata acgcgcacat gatgcgtcgc accgtcgtcg gcgagcggga catgcatgct
    53941 gccccgtgcg cgcgatacgc catcgagctc cactgacgat accccccgag taactccttg
    54001 tggattttca acgacgattt catagcgtga cgagtgatag cggaacgtga tctcaaaacc
    54061 tcgccaggca cgcgggatac agggatcgaa atgaagcctc gcgccgcgca ggcggacacc
    54121 caggatccac tctatgccag cccgatacat ccaacccgct gagccggtgt accatgtcca
    54181 accgccgcgg ccgacgtgcg gcggttcagc ataaatatcc gcggcgacga cgtagggctc
    54241 gactttgtac cggtaaacgt ccgcgcgggt gctgccatga ttgatggggt tcaagagcgc
    54301 gaaaagctca ccggctttgt ctccgtcgcc gagcgcggcg aaagctatga ccgaccagat
    54361 cgctgcatga gtatattggc cgccattttc acggatgccc ggaaggtacc ctttgatata
    54421 acctgggtcc gggctggcgt ggtcaaacgg cgggctgaac aggagcacga gaccctccgc
    54481 gcggcgcacg agatatttct cgacggctgc catggcgcgc gccgcacgag ccggctcagc
    54541 ggcaccggaa atgactcccc aggattgggc gattgaatcg atgcggcatt cggcgttgcc
    54601 ggcggatccg agcggcgtcc catcgtcgaa gtaagcgcgc cggtaccatt cgccatccca
    54661 cgcttctcgt tccagcgacg ctttgagtgc gctgacacgc agtcgccagg tctcggcacg
    54721 ctgatgctcg ccacgcgcat cggcaagctt ggcaaattcc cagagtgtgg tgtgaagaaa
    54781 ccagccgagc cagacgctct cgccctttcc tgcagatccg acccggttca tcccgtcgtt
    54841 ccagtctcct gtgccgatca gcggaaggcc gtgactgcca gctgccaggc tgcgatcgag
    54901 agcccgcgca caatgttcga acagggtgcc ggtttgcgca gatactttgg gctcgaaata
    54961 cgcctcttcc tgccccgcca cgagaacggg accgtcgagg aaaggcacga tctcgtcgag
    55021 aacaccgata tcgtctgtga cctcaagata ttggataact acatatggca accatagcag
    55081 atcatcggag acacgcgtgc gcacgccccg gcctgacggc ggatgccacc agtgttggac
    55141 gtcaccctca acgaactgcc gcgccgcagc ccgcagtaac tgcgcacgag cgatgtctcg
    55201 ttgcgcgacc attagcgcca tcacgtcctg gagctgatcg cgaaagccat atgcaccgcc
    55261 cgcttgataa aacccggctc gcgaccagac acgacaggcc agcgtctggt aaagcagcca
    55321 gcggttgagc ataaggtcca ttgcccgatc gggggttgtc acctgcacgg taccaagaac
    55381 gttatcccag tgcttggtga ccgcacgcag ggtcccgtta aggtcggccc cgcggtaaag
    55441 cgcgatcaat gttcgcgcgt cttccgtggt cgccgtctcg ccgaggacaa agacaatctc
    55501 ggcgcggtca ccggcacgca gctcaatcat cgcctgcagg gcggcgcagg gatcgagacc
    55561 ggcgcccact ttgcctgaga gactgtcccc tcgttcgagc gaacccggat gatcgggggt
    55621 gccgttgcga cccaaaaact ccgtccgatc accggtccac gcggtttggc gcccgctgag
    55681 atcggcaaac gccacgcggc cggcaaattc gctgttccaa gcgttgcgcg ccagcattgc
    55741 cccggtttcc tcgtcgatct cagtgacgac gaagggagcg gagccgctgc gcgaaacgcc
    55801 gagcacccac tcgacgtacg cggttacgga aaggcgtctg gaccgacctg agttattctc
    55861 gatagtgagg cgggagatct tgatcgggtc cgccaggggt acgaactgta acaggttaag
    55921 ggagattccg tgtgacgcgt gctcgaagcg gctgtatccc tggccatgtc gtgcggtata
    55981 cggccaagcc tcctcgcgga tcggtagcac cgtcggcccc cacagctcgc cggagtcttc
    56041 atcgcggacg tagattgtct cgccgggtgg gtcgctgacc gggtcattcg accacggggt
    56101 aagctggttc tcacggctgt tgagcgacca ggtgtaaccg gagccagact cagagacttg
    56161 aaagccgaag gacggattgg cgatcacgtt gatccagggg gccggtgtcc actgcccctc
    56221 gcccagagtc gtcacatatt cccgaccgtc ggcagtgaat ccgcccaggc cattaaagaa
    56281 ttctaggtcc ggtggcgcag gcaaacgatc cggtgcctta gtcgccggcg gacgtcgtgg
    56341 cagcggcagc gcgggcgctt gggatcgttg ctgacgttcc acttgctcag ccagggtacc
    56401 gcggcggctc aagagcactg cccgggcggc ggtctggact agcgtgcgct cctcggccga
    56461 aatgagatct cctctaagaa taaacacgcc gccgaacgac tcctgcccct catgctgcag
    56521 gcgcgactgg ctggcacgga cctgggtctc caaagcctcc tggaggattt gcgcatacga
    56581 ggacgcctgt tcgttcaaaa tcaccagatc gaccgccaac tgcttcatgc gccaatattc
    56641 gtgagcacga agcaattgac gtacgatact ctggtcttca gcctcgtcga tgcgaatgag
    56701 cacgattggc agatcgccgg aaatcccgta tcgccatagt cctgaaggcc cccggttatt
    56761 gcccgagagc accgcagacg acggtcgcag tgtcgcgtcc gagtacaaga tccggctggc
    56821 gagctcttga aagaggtagg cctcatcgac atcgataccg agatgacgta gctggacctg
    56881 ggcctgtgtc cacgccagcg tcgcggcgcg ctcgaatgtt gctggattat tgtattcgtc
    56941 ggccagggcg agcgcctcgt ctcgcgattg cgcgataagc gtcgaaaaag taacccgcgc
    57001 cgaggctccc ggcgcaagtc gtacacagcg ccggagactg gtgatcgggt cgagtaccgc
    57061 gccggctgtg ttggagagcg gatggccgtc gataaccgac atcggcgtcc gaatgccccg
    57121 accgcggccg agaaaacggc cgcgatcggt ctcgtattcc atcgcgccga cggtttctcc
    57181 ttcggcaacg atcacgtgca ccgcccaaac ctgacgctcc ccgtgcgatc gcggccggcg
    57241 cgtcgcgagc agcgcgccga ggtcagcaac atactctgtc tgcacaaaca gattggaaaa
    57301 agccgggtga gccgaatccg ctgccggcgt agccaggacg atttcggcgt acgatgtcag
    57361 ctcgatctcc cgggttcgtg ccccgaaatt tgtgatcgac acccggcgca cttccgcgtc
    57421 gtgctctggc gaaaccacta tttcaaaggt cgtcccgatc gcgccatcgc gccgccggat
    57481 ttctacgcga tcttcggaga aagtcacttc atagttgtcg ggttcgacgc cgctcggttg
    57541 atacgcggcc gaccagacgt ggccgctgga tttatcgcgt aggaaaaaat acgtccccga
    57601 ggcatcacgt gtgcggtcct cgcgccatcg tgtaaccgcc aggttgcgcc agtggctgta
    57661 tcccgaaccc gcggtagtga ccatcactga gtactgtccg ttggaaagga ggtgaacggc
    57721 tggagtcgtg tcgtgcgggg agttgaagcg acgcagcacc ggtagcacga gatcgcggac
    57781 gttcgcgacg gcccgcacct cctcgaccct gggtcgggtg accaggacgt cgcgtggagt
    57841 gcgttcctgc agcagcaact ccgtggcttg catgcgcggc tcggcgtgaa agcgcgcgcg
    57901 catcacgccg tcatggagga cgtttgcgag ggccacgagg gacatccctt gatgatgggc
    57961 catgtaggcg tgtacgacgg ccactgcttg gttctc
//
LOCUS       DASUVS010000199         6949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_81426, whole genome shotgun sequence.
ACCESSION   DASUVS010000199 DASUVS010000000
VERSION     DASUVS010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6949
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81426"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..921
                     /locus_tag="VF182_11230"
     CDS             265..921
                     /locus_tag="VF182_11230"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX6437695.1"
                     /translation="MAVRINDIAPDFTAETTQGKINFHEWIGDSWVILFSHPKDFTPV
                     CTTELGYMAKLKPEFDKRNCKIIGLSADPLTDHQGWSKDIQETQGHAPNYPIIADPEL
                     NVAKLYDMLAASETPGKRTPADNQTVRNVFLIGPDKKVKAILIYPMASGRNFDEVLRL
                     LDSLQLSVKHSVATPVNWKPGDDVIIPTSVSDEDAKKKYPQGFKTHKPYLRTVSQPGK
                     "
     gene            complement(1142..1804)
                     /locus_tag="VF182_11235"
     CDS             complement(1142..1804)
                     /locus_tag="VF182_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6437696.1"
                     /translation="MMALEVFWGSGSPFAWRVLLTLEVKRLTYQSTLLQFSKGEHKAP
                     EFLNLNPRGKVPVLKDDGFVLNESLAIMAYLDKKYPEPPLFGTSPQETGLIWRAILEM
                     EGYIVTAGDKLVRPLFFGQGLEDTDRIREAAAAIRREFRRVDTELEAASWLIGTQISA
                     VDISLFPLVQMIVRAAGKDAAKPFNFQLLPLEKNFTNIAKWVKRIAALTGYERTYPPH
                     WR"
     gene            complement(1834..2850)
                     /locus_tag="VF182_11240"
     CDS             complement(1834..2850)
                     /locus_tag="VF182_11240"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437697.1"
                     /translation="MKRLRDTVVNCIFSLVLLALIAASNAAGNAAESAKLTPLRLAYS
                     AITVNQAIPWISLEAGHFKKHGLDVELIHASSITALQALLAGEVTIVQSATDGAVSSN
                     LSGADTVFMGAILDKPLYSFIVNAKIKTPQDLKGKRVGVTRFGATPDALARATLKMWG
                     LDPATDVSMIQLNEMGLLVQGLVNGVIDAAPISIPNNIRAKNLGFVELFDMTKIGKTY
                     ITGTVVTRKRFIDGQRDVAKRFMRGFLEGMKTYQEDEEFTIKVIQKWTRAKNRDEVKE
                     AYALQAKNMLRIPRTSIEGVRTILEGLDKMPAAKTADPRRFIDFTVLDELEKEGFLKT
                     LYKN"
     gene            3240..5021
                     /locus_tag="VF182_11245"
     CDS             3240..5021
                     /locus_tag="VF182_11245"
                     /inference="COORDINATES: protein motif:HMM:NF006203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX6437698.1"
                     /translation="MAEAISHDARWSEYPGDEWSDAMVQAMKLGGIDQLFFVSGSEIA
                     FWQESIAKAQERDWPAPKLITVPHESVALNAALGSAMIRNQPAATAVHVDVGTLNYGG
                     AIHTAWRGNYPVLITAGTGPRAYAGSMTGARNSPVQWVQEPRDQGGIVRQYTKIDHRL
                     EHQDNPGFMISRLLQVAMSEPKGPVYLAAPRETAMLKLAGKTYFPTRDELGIARPVWP
                     DPADARRAAQWLIEARNPLICCATAGRNPEALAETVRLAELLALPVLDCDRVDRLNFP
                     STHTLYGTGPEIKDADALLMLEALVAYTMPSSAPRPGSKIIWVDADPVMSAYKTMDFQ
                     ADLWLPVTATTAARAIYEAATALLSKSDMSRIAERRQRLEASKREMIDNNEQAAIAAG
                     RRKPMHPRWVAYQLGKALEPNAIVLDDAISNSDFVCAYHGRAQAGTYFASGGSSGGWG
                     AGAALGAKLAAPNRDVILATGDGYFMFGTPMPALWTAAHYKAPFLTLVFVNRSYSTGT
                     NGLRRTYPTGVAVRTENFEGGLFDPPPDFAKLAESANCYGETVREPDEVGPALKRGLE
                     QVRNGTPALVAAYLPTLVEEVSLAARR"
     gene            complement(5257..5460)
                     /locus_tag="VF182_11250"
     CDS             complement(5257..5460)
                     /locus_tag="VF182_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437699.1"
                     /translation="MSRDKLEQAQSNERHGSYAFEAFEKFIDSHLVTGHDVRMFLDPN
                     GNSYSILCVTCDPRLTEIVSTRH"
     gene            complement(6560..6682)
                     /locus_tag="VF182_11255"
     CDS             complement(6560..6682)
                     /locus_tag="VF182_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437700.1"
                     /translation="MNKPLRFGSNYAEDILLILLAVFGTLGLYLFAAYFFISVL"
BASE COUNT         1645 a         1946 c         1840 g         1518 t
ORIGIN      
        1 gctgctacgg gacgattggt tgacgtgctg catccatcgg ggcaacagaa ggcaggcaaa
       61 atcgagactt cttaggacga gctcctgcag agaaaatcga tcggccaaaa aagctggact
      121 gactccccca ccgtacgtct caggatattg cctctgatgg ttaagcgtag gttaaaaccg
      181 gcaaagttct ccaaaagcat tgactgagaa ccctacacgc gatagctgta tgccagtcaa
      241 aactatcacg aaaggagaat atttatggcg gttcgaatca acgatattgc gccggatttt
      301 acggccgaaa cgacccaagg caaaatcaat ttccatgagt ggatcggcga tagctgggta
      361 atcctatttt cgcatccaaa ggattttacg ccggtctgca ccaccgagct cggttatatg
      421 gctaaactca agccagaatt cgacaagcgc aactgcaaga tcatcggact gagcgccgat
      481 cctttaaccg atcaccaggg ttggtccaag gacattcagg agactcaggg gcacgcgccg
      541 aactatccga tcatcgccga cccggagtta aacgtcgcca agctctacga catgctcgcg
      601 gccagcgaga ccccgggcaa acgcaccccg gcggacaacc agaccgtacg caacgtattt
      661 ctcatcggac ccgacaagaa ggtcaaagcg atcctgattt atccaatggc cagcggccgc
      721 aacttcgacg aagtgctgcg cctgctcgat tcgctgcaac tcagcgtcaa gcactccgtg
      781 gcgacgccgg tcaattggaa gccgggcgac gacgttatca ttccgacatc ggtttccgat
      841 gaggatgcca aaaaaaaata cccgcagggc ttcaagaccc acaagcccta tctccgcacg
      901 gtatcgcagc ccggcaagta gcgctttcct atcgctcgat atctgaaggg gtaagaaata
      961 tccgggtggc tgctgtggat taaatcgatc cacagcggcc aaaacaagct tggcgtcagt
     1021 cgaagacgtg cggtatcgcc cgcgctcact tgagagaatt aaaacaaagt ctgaatcgcg
     1081 cattagcaaa cgtctgattc agattatcac cttccgattt catcattcgt tttatctcca
     1141 ctcatcgcca atgcggcgga taggtgcgct cgtaaccggt aagcgccgcg atccgtttca
     1201 cccatttcgc gatgttggta aaatttttct ccagcggcag aagctggaaa ttgaacggct
     1261 tggcggcatc tttgcccgcg gcccgtacga tcatttgcac caaagggaaa aggctaatgt
     1321 ccactgcgga gatttgcgtt cctatcaacc aactcgccgc ttctagctcg gtgtcgacgc
     1381 gcctaaactc ccgccggatc gctgccgctg cttcgcgaat cctgtcggta tcctcgagcc
     1441 cttggccgaa gaaaagcggc cggacgagtt tatcgccggc agtaacgata taaccttcca
     1501 tctctagaat ggcgcgccag atcaaaccgg tttcttgcgg cgacgttccg aacaacggcg
     1561 gctcggggta ttttttgtcc aggtaagcca tgatcgccag cgattcgttg aggacgaaac
     1621 catcatcttt cagcacgggt actttgccgc gcggattgag attgagaaat tcaggagctt
     1681 tatgttcccc cttggagaac tgcaaaagag ttgactgata agtgagacgt ttcacttcca
     1741 acgtcaataa gacacgccag gcaaacggac tgccgctgcc ccagaaaact tccagagcca
     1801 tcatgcacct ctactttata gataaaaaag atatcagttt ttgtacaaag tttttaaaaa
     1861 gccttctttt tccagttcat caagaaccgt aaaatcgatg aagcggcgcg gatcggcggt
     1921 cttcgccgcc ggcattttgt cgaggccctc gaggatggtc cggacccctt ctatggacgt
     1981 gcgcgggatg cgcagcatgt tcttggcctg tagcgcatag gcctccttca cttcatctct
     2041 attttttgcg cgggtccatt tctgaatcac cttgatagtg aactcttcgt cttcctgata
     2101 ggtcttcatg ccctcgagga agccgcgcat gaaacgtttg gcgacgtcgc gctgcccgtc
     2161 aatgaagcgt tttctcgtga ccaccgtgcc ggtgatatag gttttgccga tcttggtcat
     2221 gtcgaataac tcgacgaagc cgagattctt tgcccggata ttattgggga tcgatatcgg
     2281 cgcggcatcg atgacgccat tgacgagccc ttgcaccaac agacccattt cattcagttg
     2341 aatcatcgac acatccgtag caggatccaa cccccacatc ttgagcgtgg cccgcgccag
     2401 agcatccggt gtcgcgccga accgggtgac gccgactctc ttgcccttga gatcttgtgg
     2461 cgttttgatc ttagcgttga cgataaagct gtacagcggc ttgtcgagaa ttgcccccat
     2521 aaaaacggtg tcggcgccgc tcagattgga gctcacggcg ccatctgtcg cggattgcac
     2581 gatcgtcacc tcgcccgcga gcaatgcctg cagcgccgtg atgctgctgg cgtgaatcag
     2641 ttcgacgtcg aggccgtgct ttttgaaatg gcccgcctct aagctgatcc atggaatcgc
     2701 ctggttaacc gtgatcgcgc tgtaggcaag ccgcaggggc gtgagcttcg ctgattcggc
     2761 cgcgttgccg gcggcattcg atgcggcaat caaggccagc agaaccagcg aaaatatgca
     2821 attcacaacc gtgtctcgaa gtctcttcat acatcctccc accgttcgcc ttagcaatat
     2881 tcgacttttc tgctgtagat caaggggaat ttgttcctag tcacgaggtt caccttgaac
     2941 atcgaatcag gagcagcacc tgatcgagtg accgacatgc agtctgagaa gctcatggac
     3001 acgcaacctg cagatttcac ttactcggga tctgctccga tcccgcctga attccgatac
     3061 gaccattcgg caatgacgaa tgcagttatg agaaagtatt tttaaacact cgttgcctcc
     3121 aaacaaacct caaaagtatc aagccgagtt caaaaagggg ttgccaaaac actgggctat
     3181 ggaggatagt ccgggtctag cctttcgaaa acgaaaatag tcttgaagga gatacgttga
     3241 tggctgaagc aatctctcac gatgctcgct ggagcgaata tcccggcgat gaatggtccg
     3301 acgccatggt gcaggcaatg aagcttggcg gaatcgatca actgttcttt gtttccggct
     3361 ccgagatcgc tttttggcag gaaagcattg ccaaggctca ggagagagat tggcccgcgc
     3421 ccaaattgat caccgttcca cacgagagcg tcgcgctcaa tgccgcgctc ggcagcgcca
     3481 tgatcaggaa tcagccggca gccaccgcag tgcacgttga cgtcgggaca ttgaattacg
     3541 gcggcgcgat ccacaccgcc tggcgcggaa attacccggt attgatcacc gctggaacag
     3601 gaccgcgcgc ttatgccggt tcgatgaccg gcgcccgcaa cagccccgtg caatgggttc
     3661 aggagccgcg cgatcagggc ggcatcgtca ggcaatacac caaaattgat catcgcttgg
     3721 aacatcagga caatccagga ttcatgatca gccgcttgtt gcaggtcgcc atgagcgagc
     3781 ccaaagggcc ggtctatctt gctgcgcccc gggagacggc gatgttgaag ctcgcaggca
     3841 agacgtactt cccgacccgc gacgagcttg gaattgcgcg tccggtgtgg cccgatcctg
     3901 ctgacgcgag gcgagcggcg cagtggctca ttgaagcgcg caatccgctc atctgctgcg
     3961 ccaccgcggg acgcaatcct gaggcgctcg ccgagactgt tcggctcgcc gagttgctgg
     4021 ccttgccggt actcgattgc gaccgcgtcg accggctgaa cttcccgagc acgcacacgc
     4081 tttacggcac cggacccgaa atcaaggatg ccgatgcctt gctgatgctc gaggcgctgg
     4141 tggcttacac gatgcctagt agcgctccgc gacccggcag taaaatcatc tgggtcgatg
     4201 ccgatccagt gatgtcggcc tacaaaacca tggatttcca agccgatctc tggcttccgg
     4261 tgaccgccac aaccgcagcg cgagcgatat acgaagcggc gactgccctg ctttctaaga
     4321 gcgacatgag ccgaatcgcc gaacgacgtc agcgattaga ggcaagcaag cgcgaaatga
     4381 tcgacaacaa cgaacaggcc gccatcgcag ccggccggcg caagcccatg cacccgcgct
     4441 gggttgccta tcagctggga aaggctctcg agccgaatgc catcgtgctc gacgatgcga
     4501 tcagcaactc ggatttcgtc tgcgcgtacc atgggagagc tcaagccggc acctacttcg
     4561 cgagcggcgg atcatccggc gggtggggcg ccggcgcagc gttgggagcc aagctcgccg
     4621 cgccgaatcg cgatgttatc ctcgcaaccg gcgacgggta tttcatgttc ggtacaccga
     4681 tgccggcctt gtggactgcc gctcactaca aagcgccgtt tctgacactt gttttcgtca
     4741 accgctcgta cagcaccgga accaacggct tgcgcagaac ctatccgacc ggcgtcgccg
     4801 tgcgcacgga aaattttgaa ggcggcctat tcgatccacc gcccgacttt gccaaactcg
     4861 ccgagtccgc gaactgctat ggcgagactg tgcgtgaacc cgatgaagtc ggcccggcgc
     4921 tcaaacgagg attggaacag gtgcgcaatg gcactcccgc tttggtcgcc gcatatttgc
     4981 cgactttggt cgaggaggtg tcgctggcgg cgcggagata aaaaactatg tgatcggggt
     5041 aagattgagg taggcggatc attcgtgagt tcacatttgt ttgcggaccc gaggagcgcc
     5101 atcggatctc aaataactcc cgctattacg ggaaataaga tagctatctg cttgatcgaa
     5161 cgtagccatg gtaagcgtcc ggtgaaagcg cactcgccaa atgagggatg agaacgacac
     5221 gcacttcggt atgacagtcg gaagttgctc gaaactttaa tggcgggtcg atactatctc
     5281 ggtcagtcga gggtcacagg tgacgcacag aatagaatag gaattgccgt tcggatccaa
     5341 aaacatacgg acatcgtggc ctgttaccaa gtggctgtcg atgaacttct cgaacgcttc
     5401 aaaggcataa gaaccatgac gttcattgct ttgtgcttgc tctaatttgt ccctgctcat
     5461 tttgcctccc ggtgttgcac gagtaaaaat gcgccgggta tatagcatag cttcttgcgc
     5521 gagtcacgcc ctgctctttc gagtaagacc gggtagtgcc ggttggcagg atcactgcag
     5581 ctaaatcaag tctgacctct attcggtgcc ccaatagtct gtccttaaga tcttccgaaa
     5641 ccagctttcg gtgaatcatc cgtcgtccac aggttgcatc agacttgatg ctgctgatgt
     5701 atcgccttcg gacctcgtac ctgatggccg atctgccagt cccaggacgc tctacgcact
     5761 gctttctggc gagataccgt catctaaggc atggctggga gacaccctcg aagttgactt
     5821 ccgactgttt tagaatcaac aatctcatga gtaacatcga gaagcggtag ttcgcttaaa
     5881 caactcttgc gctcgttacg tccgaaagga gcataaacta tggcagagca gaactgcgcc
     5941 cacgccggtt gcgcctgtaa ggtcaaacat ggacagaccg tctcacgggg aaatgacaat
     6001 tactgcagtg atcactgcgc gcaagccggc acagctcgag cgggtcagtg tgtgtgcggc
     6061 catccggatt gcgagactgc cgggatgacc cgatgaacaa tcccgttctc gaccggagtc
     6121 ggtcagaacc caaaatgcag ctcaggtgct gcagagattg accccaaaag cctttcagtt
     6181 ttcataactg gcccgcaaca gcggtcgatc attccagacg ccacgactca tcagcagggc
     6241 gttatgcttg ggcaagtccg ctccggattt cagtgggtga gactgtcaac agtttcgccg
     6301 atcgttattt cgcgaacgtg tgcccagaac gttgcactat cggctaactt cgaaacccct
     6361 tgtttacaga ggagtatgga tctggaggta attcccctct ggatcgaaag aataaaccga
     6421 gcttcccgac tcgcgcacgt gttcctcgta cataattttg acgcctgatt tcttcaggtc
     6481 ggctacagcg gttgtaaaat cctctttggg cagctccagt ctggcggatc accaagcccg
     6541 caaaaggatc aagccttatc taaagcacgc tgataaagaa atatgcagca aacaaataaa
     6601 gccctaatgt accgaagacg gctaatagaa taagcagtat gtcttcggca tagttcgacc
     6661 cgaacctgag tggcttgttc atacgacgtc tcccattgat gctcaaccaa attgcgatgg
     6721 gcagatcgct tccttcaaaa cgaacgctta agtggggccg gcaacactaa agagtgacga
     6781 aatacaggcc cccataattt gccagatccc gagatctttt tactcgagca attccttcgg
     6841 ggctaacaat tctcggattt tgcgctggag atccgcaccc gcaaatggtt tgacaatgta
     6901 gccagtacag ccggcgtcca aacacgactt caggtcctcc gaacgaaag
//
LOCUS       DASUVS010000200        10061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_81574, whole genome shotgun sequence.
ACCESSION   DASUVS010000200 DASUVS010000000
VERSION     DASUVS010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81574"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VF182_11260"
     CDS             <1..443
                     /locus_tag="VF182_11260"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437701.1"
                     /translation="ALDRIEWMPPRASKRETAPVILQAELQKLMWEKAGPFRTGEKLS
                     EALRRIRDMRERELPDLKIASDGAFNLDLQDWFELRAMLTTAEAVVTSALARTESRGA
                     HQREDYPELDPLYLKNQLLRLEEGALKSAWIEPVRRVAGLAKNG"
     gene            436..801
                     /locus_tag="VF182_11265"
     CDS             436..801
                     /locus_tag="VF182_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX6437702.1"
                     /translation="MVEGSISKTVLRVRRGGPADAPRFDDFEVPFEEGMSVLDALRWI
                     RLHRDSSLAIRYSCINANACKTCMALIDGEVEYTCIAKLLPGIVTVEPLPKRRLVRDL
                     VTDVVPEEEKACLAEKQPD"
     gene            complement(984..1874)
                     /locus_tag="VF182_11270"
     CDS             complement(984..1874)
                     /locus_tag="VF182_11270"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HEX6437703.1"
                     /translation="MSLYDEMIEGGIDVHCHIDFEFSAKERKREPEFLWLPKAEAMGM
                     RGVVLKSHWWPTINVVPYILSSVETRVRLWSSVALNSTAGGPNPCIIESCAQLGGKMV
                     FLPTWSARNDVERKGFSNRLKSYYKRHAQLDNPNYYFLDDKGKLISLGYEIIECCKAH
                     DLTLGMGHVSWQESLAFIEAARDMGYSHRIIVNHVQSHIIAMPLDAMQRAAAMGAFLE
                     TCWNALAPGRMDSPELVQQFRQIGLQQIIASTDYFRPYSPNPAELFRMFLGMLHEGGL
                     SKDEVKQVACANPAKLMGLE"
     gene            complement(1966..3261)
                     /locus_tag="VF182_11275"
     CDS             complement(1966..3261)
                     /locus_tag="VF182_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HEX6437704.1"
                     /translation="MNTASREFQIMVKTVGAICNLDCHYCYYLEKEELYPKGSNFRLS
                     DETLERYIIQHIQATPKELVSFSWHGGEPTLLGVDFFRKAVELQKKHLPADKKIINGI
                     QTNGTTLNQEWCEFFAQETFYVGLSLDGPQDLHDHYRVTKGQKPTHKQVVQAFQMLRR
                     HKVHVDLLCVVHDINVKHPIAIYRYLKEIGGQYLQFLPLVEKPDKPGAPAGSRSVPAA
                     AYGEFLSAIFDEWVRHDIGKVFIQLFDESVRPFLGMEHALCIYRETCGDVPVIEHNGD
                     FYSCDHYVEPAYKIGNIYEQTLASMIDHPAQHEFGRKKWTSLPRFCMECEVRSMCNGG
                     CPKDRFIKTPDGEDGLNYLCAGLKGFFTHSKPYLKKFAELVDAGEPGEKLMQIVRAAD
                     AKKIVPQAGRNDPCPCGSGKKYKRCCGAGKIKSRGSVIL"
     gene            complement(3536..4216)
                     /locus_tag="VF182_11280"
     CDS             complement(3536..4216)
                     /locus_tag="VF182_11280"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX6437705.1"
                     /translation="MLGPNRTEKKFRASVEDTAGEIRKAILQGKIAPGEQLSEEKLAA
                     SLRASRVVLREALRALEAQGYVTFLPSNHVVASNPSREDMHDYYTIAGALEGLAARLA
                     VERAQPEEIARLRELHQLLREACHKGDALQYFDANSNFHRYIAELSHNERLYQLVEEL
                     RREIQKTRLLSLHMPQRLDYSMREHDQIMDAFLKKNPQLAESMMVQHLTNHLKALGKA
                     LDSGSESR"
     gene            complement(4219..5727)
                     /locus_tag="VF182_11285"
     CDS             complement(4219..5727)
                     /locus_tag="VF182_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX6437706.1"
                     /translation="MQSVYVAGIGMTKFGKDPRSLAEICHDAAQTALTSSEIQEVEAI
                     YIGVMNPEEFTGDSNIASHIADALGLTGIPAVRIETASSAGAAAIQAGFQAVASGYYH
                     RVLVLGGEKMTHLSTSATTRVLAGVIDQEERQCGATMPALAAMITEKYREKFNLSQSR
                     LETMLCAVALKNHGNGAHNPMAQFQKPISRETYLASKYVATPLRLYDCAPITDGAAAV
                     MLTSDKTDVRLAGVGQGTGPLSLRGRQVFTSFPATRMAAARAYQMAETSPQGIDFAEV
                     HDAFTPFEIITTEDLGFFVPGKGGDAVLDGVTAAEGRLPVNPSGGLKARGHPVGASGL
                     AQIVEVVKRLRGENGSKRQFNRGLTQSTGGLASNNFVTILERTEASLPRAPGFPQPSR
                     NPAKRRPTTMPMSNEGVIETFTILYVTPDGFLPPLALALIREQNGQLIMAQGEQSKHL
                     KIGREVYLKRTEDYYLFTLKGRFRMVQDSLKRNLWQRARALVKKPMPAGGKL"
     gene            complement(6038..7897)
                     /locus_tag="VF182_11290"
     CDS             complement(6038..7897)
                     /locus_tag="VF182_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6437707.1"
                     /translation="MTTLNKKAGVRRRKLRGSLAPATAVRAEKFSSLLILPTLVLIIA
                     TLYWGQAFLIPVALSILLTFLLTPVADGLERIGLGRLASVMLIVILAFSLLAAIGWMV
                     TLQLTSVANELPTYRSNIREKISDIRGAGKGGAIEKIRETAEEVKEEFSKEETTKPSS
                     KPREVVVQSEESSTFWPLPLATAPMLERMASGGLVIVLVIFMLIQRENLRNRLIRLVG
                     YGRLTITTKALEEAAQRISRYLLMQSIINASFGIAVGLALYLFGLPYALLWGFLAAVL
                     RFIPYVGPVAAALLPSTLSMAVFPGWTWPLLVVATIIALELINNMVLEPLLYGESAGV
                     SEVGLLVAVAFWTWLWGPIGLVLSTPLTVCVVVISKYMPQLDFIGVLMSDEPVIEPSI
                     SYYQRLLAMDQDEAAEIVEEHLKSHPKEQIFDDLLIPALNYLKRDFGFGKMAESEQQF
                     VLQATRQIIEDLDNSGTGKASFEAPSANMQTGGEQPADATRKIRIMGCPVRDEADELS
                     LLMLRQLLDPVNYELEIIADELLASEIIDQIAEKKPALVCLVSLPPGGLAQTRYLCKR
                     LRARFPETKLIVGRWGSRTDNSNALIDAGADKSGNKILETRDHIIQLSQILPR"
     gene            complement(8202..9704)
                     /locus_tag="VF182_11295"
     CDS             complement(8202..9704)
                     /locus_tag="VF182_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX6437708.1"
                     /translation="MHDSNKAQPWPVLGDMPPNEFRKSLHRIADWVADYREGIEQLRI
                     SPDVIPGDIKARLPQAPPDVAVPFNNVFQDFEKLILPGIVHWGHPAFFGYFGSTTTSA
                     GIIGEMLMGALNVSTMTWRTSPAATELETLVLDWLRQLLGLSSDYTGVVYDTASVAVM
                     HALAAARENLGLDARARGLCGRAEVPALRIYASDQAHSSVEKAAITLGIGERNVRRVS
                     CEANYRMDISALRSAIETDLRAGLLPMAVIATVGTTSTTSVDPIPEIAAVCNEHRLWL
                     HVDAAYGGAMALLPEGRHVMKGVEAADSIVVNPHKWLFVPLDFSALYVRRPELLRATF
                     SLVPEYLRGDAEQGERNYMDYGIQLGRRFRALKAWMVLRSFGSAGLAARIREHLRLAR
                     LFANWLDADSEFELLAPVTMAVICFRMTPRKLVTRQAADQELNGFNRRLVEAVNRTGK
                     AYLTYTNLNGHIAMRLAVGNILTTQQHLAMVYDLIKCEAARLDSSTESSQ"
     gene            complement(9738..>10061)
                     /locus_tag="VF182_11300"
     CDS             complement(9738..>10061)
                     /locus_tag="VF182_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437709.1"
                     /translation="LALGLINGAHAVLAFFFAISIGAGLLAGRGLLRDRAYRFFPIYV
                     IASAPLWIFERSLSVYWALYWRLAYGGYPFGEQLLSKGTGRAWTAARAFMRKRQPLTN
                     QSRRA"
BASE COUNT         2210 a         2766 c         2806 g         2279 t
ORIGIN      
        1 ttgctcttga tcgaatcgag tggatgccgc cgcgcgcttc taaacgagaa accgcgccgg
       61 ttatccttca ggccgaactg caaaagctga tgtgggagaa ggccggacca tttcggaccg
      121 gtgaaaagct ttccgaggcc ttgcggcgga tccgcgacat gcgtgagcga gagttgcccg
      181 atcttaagat tgccagtgac ggcgccttca atttggatct tcaggattgg ttcgagctcc
      241 gtgccatgct tactacggcc gaggcggtgg taacatcggc cctggcgcga accgagagcc
      301 gcggcgcgca tcagagggaa gactatcccg agcttgaccc gctgtatctc aagaatcaac
      361 tcctgagact cgaggagggc gcgttgaagt ccgcctggat cgaaccggtc cggcgtgttg
      421 cggggctagc gaaaaatggt tgaaggctca atcagcaaaa ccgttttgcg cgtccgccgc
      481 ggcggtcccg ccgatgcgcc gcgtttcgat gatttcgaag ttccgttcga agaaggcatg
      541 tcggtgctcg atgcgctacg ctggatccgg cttcatcgcg atagctcgct ggcgattcgt
      601 tacagctgca tcaacgccaa tgcgtgtaaa acctgcatgg cgctaatcga tggggaagtc
      661 gaatatacct gcatagccaa actgttaccc ggcatcgtta ccgtggagcc actgccaaag
      721 cgccggctgg ttcgcgattt ggttaccgat gttgtgccgg aagaagaaaa agcgtgcctt
      781 gcagagaaac aaccggattg aactatttcg tctgatgagg tcgtttatcc gagaatatcc
      841 gtcggttgta acgacgtgcc cggaatcttt caggaccagc ttgcaatcac ctgtccgtcg
      901 ctcatgcaag aaggcttgcg atgataaccc tcgataactt gctgatcatc ggctgtgcgc
      961 ggttctgcat tgccagtcgt tggttattct aaacccatta actttgccgg gttcgcacag
     1021 gcgacctgct tcacttcatc tttgctgaga ccgccttcgt gcagcatacc aaggaacatc
     1081 cggaagagct cggccgggtt cggactgtat gggcggaagt aatccgtgct ggcgataatt
     1141 tgctgcaagc caatctggcg gaactgttgc acgagctcag gggaatccat gcgcccggga
     1201 gcaagcgcat tccagcaggt ctcaagaaaa gctcccattg ccgcagccct ctgcatggcg
     1261 tccagcggca tagcgatgat atggctttgc acgtggttaa cgataatccg atggctataa
     1321 cccatgtccc gcgccgcttc gataaaagcg agagattcct gccaggagac gtggcccatc
     1381 ccaagagtta ggtcgtgcgc tttacagcac tcaataattt catagcccag cgaaatcagc
     1441 tttcctttgt catcgaggaa ataatagttt gggttatcga gctgcgcgtg cctcttgtaa
     1501 taggatttta ggcggttgct aaagcctttg cgctcgacat cgttgcgcgc gctccaggtc
     1561 ggaagaaaaa ccatcttgcc gccaagctgg gcgcaggatt cgatgatgca tgggttggga
     1621 ccgccggctg tgctattgag cgcgacgctg gaccagaggc ggaccctcgt ctcgaccgac
     1681 gaaagaatat aggggacgac attgattgtt ggccaccagt gcgacttgag aactaccccg
     1741 cgcattccca tcgcctccgc ttttggcagc caaagaaatt ccggctctcg ctttcgttct
     1801 ttggcggaaa attcgaagtc gatgtggcaa tgaacgtcga tgcctccttc gatcatctca
     1861 tcgtataggc tcatgctcag actcctccga acgtagttgg tagagcgtag acgttgtgct
     1921 gccggtgagt gttatcagaa acgggctgag ctcgtgtact ctgtcctata gtattacgga
     1981 gccccgcgat tttattttgc cggcgccaca gcatcgtttg tatttcttgc cactgccgca
     2041 ggggcaggga tcgttgcgac cggcttgagg gacgatcttt ttggcgtcgg cggcgcggac
     2101 gatttgcatc agtttttcgc ccggttcgcc ggcatcgacg agttccgcga attttttcaa
     2161 atagggtttg ctgtgcgtga agaaaccttt caacccggca cagagataat tcaacccatc
     2221 ttcgccgtcc ggcgttttga taaagcgatc cttcgggcag ccgccgttgc acatgcttcg
     2281 aacctcgcac tccatgcaaa agcggggcag gctcgtccat tttttcctgc cgaattcgtg
     2341 ctgcgccgga tgatcgatca tcgatgccag agtctgttca tagatgttgc cgattttata
     2401 tgccggctcg acgtagtggt cgcatgagta aaaatcgcca ttgtgctcga tcaccgggac
     2461 gtcgccgcaa gtctcgcggt agatgcagag cgcgtgctcc atgccgagaa agggccgcac
     2521 cgattcgtcg aacagttgaa tgaaaacttt gccgatatcg tggcgcaccc attcgtcgaa
     2581 gatcgccgag agaaattcgc cgtaagcggc agccggcacc gaacgggagc cggcgggagc
     2641 gccgggttta tccggctttt ccacgagcgg cagaaattgt aagtactggc cgccgatttc
     2701 cttcagatag cgatatatgg caatgggatg tttcacattg atatcgtgga ccacacacag
     2761 caaatcgaca tgaactttgt gccggcgcag catctggaaa gcttgaacaa cttgcttgtg
     2821 ggtgggcttc tgccctttgg tcacgcggta atgatcgtgc aggtcttgcg gtccgtccaa
     2881 gctcaacccg acgtagaacg tctcctgagc aaaaaattcg caccattcct ggttcagcgt
     2941 cgtgccattg gtctgaatgc cattgataat tttcttgtcc gccggcaagt gctttttttg
     3001 cagctccacg gctttgcgga aaaaatcgac gcccagcagt gtcggctcgc cgccatgcca
     3061 ggagaagctg acgagctctt tgggggtcgc ctgaatatgc tggatgatgt agcgttcgag
     3121 agtttcatca ctaaggcgga agttggatcc tttgggataa agctcctctt tttcgaggta
     3181 atagcaataa tggcagtcta gattgcagat ggccccaacc gtcttgacca tgatctgaaa
     3241 ttcacgagac gctgtattca accgttacct ccgacgatcc gaaaagtggc cggcgcagcc
     3301 ggccaatcca tggcaggact ctacttaaaa atagttcgga tgtcatcccc tgggtaaact
     3361 tcgccgccct cggcggttct tgtgggaatc ggctggatca tgtgaagggt ttaagacgcg
     3421 acgagaaaaa cacgtctgga actcagatga agtatgagga ggatcctaca catgtattac
     3481 tgcattactt ggttgcctgc agattgtgca cgatgttcga gcgcaagcag aggatctatc
     3541 gcgattcgct accggaatcg agcgcctttc cgagggcttt taaatgattg gtgagatgtt
     3601 gcaccatcat gctttcggcc agctggggat ttttcttgag gaatgcatcc atgatctgat
     3661 catgctcgcg catcgagtag tcgagtcgtt gcggcatatg caacgacagc aggcgggttt
     3721 tctgaatttc acgccgcagt tcctcgacga gttggtaaag ccgctcattg tgcgacagct
     3781 cggctatgta acgatgaaaa ttgctgttgg catcgaagta ctgcagcgcg tcacccttgt
     3841 ggcatgcttc tctgagaagc tggtgcagct cgcgcaggcg agcgatctcc tccggctgag
     3901 ccctctcaac ggccaggcgt gccgccaagc cctcgagcgc gccggcaatg gtgtagtaat
     3961 cgtgcatgtc ctcgcgcgat ggattgctgg cgactacatg gttgctgggc aaaaacgtca
     4021 cgtagccctg cgcttccaag gctcgtagcg cttctcgaag caccacgcgg ctggcgcgga
     4081 gagaggccgc cagcttttct tccgatagct gctccccggg cgctatctta ccttggagga
     4141 tcgcctttcg aatctcaccg gcggtgtcct caaccgatgc tctaaatttt ttttctgttc
     4201 gattggggcc gagcatgatt ataacttacc gcccgccggc atcggctttt ttacgagagc
     4261 tctggcgcgt tgccagagat ttcgttttag agaatcctgg accatcctga aacgtccctt
     4321 gagggtaaag agatagtagt cctcggttcg cttcaggtaa acttcacggc cgatcttcag
     4381 gtgcttgctt tgttctccct gcgccataat tagctgcccg ttctgttcgc ggatcaaggc
     4441 tagggccagc ggcggtaaga atccgtccgg ggtaacgtat aagatcgtga aagtttcgat
     4501 gaccccctca ttgctcatgg gcattgtcgt cggccggcgt ttcgcggggt tgcgagaagg
     4561 ctgcggaaat cccggcgcgc gcggcagcga tgcttcagtg cgttcgagaa tcgtgacaaa
     4621 attattgctg gcaaggccgc cggtgctctg ggtcagccct ctgttgaatt gccgtttcga
     4681 accgttttca ccacgcaacc ttttcaccac ttcaactatc tgcgccaatc cgcttgcgcc
     4741 gaccggatgt ccccgcgcct tcaaaccgcc tgacgggttt accggtaagc gtccctcggc
     4801 tgcggtcacg ccgtccagga ccgcatcgcc gcctttgcct gggacgaaaa accccaaatc
     4861 ttcagtggtg ataatctcga acggcgtgaa cgcatcgtgc acctcggcga agtcaattcc
     4921 ctgcggcgaa gtctcagcca tctgataagc ccgcgccgcc gccatccggg tggctggaaa
     4981 ggaggtaaaa acctgtctgc ctcgcaggct gagcggcccg gttccttgac cgacacccgc
     5041 cagccggacg tcggttttat cgctggtaag catgacagcc gcggcgccat cggtgatcgg
     5101 cgcgcagtcg tacaggcgca gcggtgtggc gacgtatttg ctcgccagat aggtctcgcg
     5161 gctgatcggt ttctgaaact gcgccatcgg gttgtgcgcg ccattaccat gattctttag
     5221 cgccacggcg cacaacatcg tctccagccg ggactgcgat aagttgaatt tctcgcgata
     5281 cttctccgtg atcatggcag ccaatgcggg catggtcgcg ccacactgtc gttcctcttg
     5341 gtcaatgacg ccggccaaaa ctcttgtcgt cgcgctggtg ctcaagtggg tcatcttctc
     5401 gccgccgaga actaaaactc ggtgatagta accggatgcc acggcttgga aaccggcctg
     5461 aatcgccgcc gcgccggcgg aagacgcagt ttcaatgcgc accgccggaa ttccggtcaa
     5521 ccccagggca tcggcgatgt gcgaagcaat attgctgtca ccggtaaatt cctcgggatt
     5581 catgacgccg atataaattg cttccacttc ctgtatttcg gaagaggtta gggccgtttg
     5641 agccgcgtca tggcagattt cggccaagga tctcggatcc ttgccaaact tcgtcatgcc
     5701 gattccggca acataaaccg attgcataca agcaattata ctgaccaaga cgctaattcc
     5761 aagtagagtg gcgaccgacc taggttgaaa ttagccgaat cgtgagggga aacaaaaata
     5821 ctgaatatga agattttgtt acgctggatg acagatccaa agagagtatg cgcaatctcg
     5881 attgacgtca cgtaatttcg gtgcgattgg cgcagaatta tatagaaatt gacagagctg
     5941 tgagaaggta gagcagcgcg gtgactgcgc agaacttacc cagctggcct gattaatacc
     6001 cgtgtgtcgc cagtcacaac gcactaatcg aaaacgatca gcgcggaaga atctgactta
     6061 actggatgat gtgatcgcgt gtctcgagaa tcttattccc gctcttgtcg gcgccggcat
     6121 cgattagagc attgctattg tcggttcggc tgccccatcg accgacgata agtttagtct
     6181 cgggaaatcg cgcgcgtaac cttttgcaga gatatcgggt ctgagctaag ccgccaggcg
     6241 gcaaagagac aagacaaact agagccggtt ttttttcggc aatctggtcg ataatctccg
     6301 acgcgagtag ctcatcggca ataatctcga gttcataatt gaccggatcc agtagctgcc
     6361 gcagcatcag cagacttagc tcgtcggctt cgtcgcgaac cggacagccc atgattcgga
     6421 tcttgcgcgt ggcgtctgcg ggctgctcgc cgccggtttg catgttcgca gacggcgcct
     6481 cgaaggaagc ctttcccgtt ccggagttgt caagatcctc gattatttgc cgcgtggctt
     6541 gcagaacgaa ctgctgctcg ctttccgcca tcttaccgaa accgaagtct cttttgaggt
     6601 agttcaaggc cgggatcagc aggtcatcaa agatctgctc tttaggatgt gacttcaaat
     6661 gctcttcgac gatttccgcc gcttcgtcct ggtccatagc cagcagcctt tgatagtaac
     6721 tgatgcttgg ctcgatcacc ggctcatcgc tcatcagaac tccgatgaaa tccaactggg
     6781 gcatatattt actgataacg acaacgcaca ccgtaagcgg tgttgagaga acaagtccga
     6841 tgggtcccca tagccaagtc cagaacgcga ccgcaaccag aagcccaacc tcggaaaccc
     6901 ctgcgctttc accataaagg agaggctcca ggaccatatt gttgatcagt tctaaagcaa
     6961 tgatcgtggc aaccaccagg agtggccaag tccaacccgg aaacaccgcc atactgaggg
     7021 tgctcggtag aagcgctgcc gcaaccgggc ctacataggg aatgaaccgc agtaccgcag
     7081 ccaagaaccc ccacaggagc gcgtatggca ggccgaacag gtagagcgcg aggccgactg
     7141 ctattccaaa cgaggcattg atgatggatt gcatgagcag atatcggctg atccgctgcg
     7201 ctgcttcttc gagagctttg gtggtgatcg tcagacgacc gtagccgacg agacggatca
     7261 gacggttgcg cagattttca cgctgaatga gcataaaaat caccagcacg atcaccaggc
     7321 cgcctgaagc cattcgttcc agcatcggcg ccgtggctaa cggaagaggc caaaatgtcg
     7381 atgactcttc ggattgaacg acgacttccc ggggctttga cgaaggtttt gtggtctctt
     7441 ctttactgaa ctcctctttg acctcctcgg cagtctcgcg aatcttctcg atagcgcctc
     7501 ccttaccggc gccgcgtatg tcgctgattt tttctctaat gttgcttcta taagtgggca
     7561 actcgttggc gacgctggtc agttgaagcg tcaccatcca gccgattgcc gccaagagcg
     7621 aaaatgccag gatgacgata agcatcactg aagccagtcg gcccaagcca atccgttcta
     7681 aaccgtcggc aaccggcgtt agtagaaacg ttagtaaaat agaaagcgcc accgggatga
     7741 gaaatgcttg gccccaataa agggtagcaa taatcaaaac cagtgttgga aggattagta
     7801 gcgatgagaa tttctcggcg cggacggcag tagcaggcgc aagactacct cgtagtttcc
     7861 gacgccttac accggccttt ttattgagcg tggtcaatca ggtatccttc tcaaggttct
     7921 taaaagcact cgtacgctgt atcgacgcga gaccattcat cggcccagag aactctagga
     7981 aagcggtgcc cgcccccaat gtgtgagagt tcttggcctg gtcccgaatc gccggcgcta
     8041 ctttgactct gtccgaggtt ggtccctggc gcaagacgag cttgcagcgc ctgtaggtcg
     8101 ccccgcccct ttacttgatt agatgatctc cgagaactct cgtgctctaa cgagccgaag
     8161 gtcgtcacga aattccgcag aagtagcgac gacgttcgca gctattgaga gctctcggtg
     8221 gatgaatcaa ggcgagcggc ctcgcacttg atgagatcat aaaccatcgc gagatgttgt
     8281 tgcgtggtta gtatgtttcc gacggctagc cgcattgcaa tatggccatt tagattggtg
     8341 taggtcagat aggctttgcc ggtgcgattg accgcttcga cgagccgtcg attaaaaccg
     8401 ttaagctctt ggtccgccgc ctgtctggtt acaagcttac ggggcgtcat gcggaagcag
     8461 atcacagcca tcgtgactgg ggcgagcagc tcaaactccg aatcggcatc gagccagttt
     8521 gcgaacaagc gcgccagacg aaggtgctcc cgaattcgag cggcaagtcc cgcgctgccg
     8581 aagctgcgca ataccatcca cgccttgaga gcgcgaaatc gacgaccgag ttgtatgccg
     8641 taatccatgt agttgcgctc gccctgttcg gcatcaccgc gcaggtactc ggggacgagc
     8701 gagaaggtgg cgcgcaggag ctccggccgc cgcacgtaga gcgcgctgaa gtcgagcggg
     8761 acgaataacc acttatgtgg attgacaaca atggagtcag cggcctcgac tcccttcatt
     8821 acgtgacgac cttcgggcaa cagagccatc gcgccgccat aagcagcatc aacatgcagc
     8881 cacaagcggt gctcgttaca gaccgcggca atctcaggta tggggtcgac gctggtggtc
     8941 gaagttgttc cgactgtggc tatcaccgcc atcggcagca gtccggcgcg caagtccgtt
     9001 tcgattgcgc tgcgaagcgc agatatgtcc atgcgatagt tcgcttcgca ggacactcgg
     9061 cgcacatttc gttcgccgat gccaagagtg atggcagctt tctctaccga gctgtgcgcc
     9121 tgatccgacg catagatgcg taaggccgga acttcggcac gcccgcataa gcctcgagcg
     9181 cgggcatcga gacccaagtt ctcacgggcc gccgccagcg catgcatcac agctacggaa
     9241 gcggtatcgt aaaccacgcc tgtatagtca ctggagagcc cgagaagttg gcgcagccaa
     9301 tcgagaacca atgtttcgag ttcggttgcc gccggcgagg tgcgccacgt catcgtgctg
     9361 acgttaagtg cgcccatcag catctcaccg atgatgccgg cagaggttgt ggtcgaaccg
     9421 aaatagccga agaatgccgg atgtccccag tgcacgatgc cgggaaggat taatttctcg
     9481 aaatcctgaa agacgttgtt gaacggcacc gcaacgtcag gtggcgcttg cggcagccgc
     9541 gccttgatgt cgcctggtat aacgtctggc gaaattctta gctgttcaat gccttctcgg
     9601 tagtctgcaa cccaatctgc aattcgatgc agcgacttgc ggaactcatt tggcggcata
     9661 tctccgagca cgggccaggg ttgcgcttta ttgctgtcgt gcatggttct ctctggttgc
     9721 cttagtcaca agctggctta ggcccggcgc gactgattcg ttaatggctg ccgctttcgc
     9781 ataaatgccc gcgctgcggt ccaggctcgc cccgtccctt tcgataataa ttgctcgccg
     9841 aacggatagc cgccgtaagc caggcgccag tacagcgccc aatagacgct cagcgaacgc
     9901 tcgaatatcc agagcggcgc agaggcaatc acataaatag gaaaaaaacg ataagctcgg
     9961 tcgcgcaaca acccgcggcc ggcgagcagc cctgcgccga tcgatatggc aaagaaaaac
    10021 gccaacacag catgcgcgcc attgatcaag ccgagtgcga g
//
LOCUS       DASUVS010000201         3391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_954778, whole genome shotgun sequence.
ACCESSION   DASUVS010000201 DASUVS010000000
VERSION     DASUVS010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954778"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(352..1920)
                     /locus_tag="VF182_11305"
     CDS             complement(352..1920)
                     /locus_tag="VF182_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015200339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6437710.1"
                     /translation="MSLLRFFLEHRAEVVALILEHLFLVGVSIAIALLIGVPLGILLT
                     RKPALSKPVLGFANIMQTVPSLALFGFLIPVNLMLFDIRIIGGIGARTAIAALVLYSL
                     LPIIRNTFTGISGVDPAIREAGRGMGMTDRQLLLQVELPLARSVIIAGVRVATVICVG
                     TATIAAAIDAGGLGRYIFRGLRANDNVLILAGAVPAALIALIADLFLGSLERATRSRG
                     AASRLRKFVWPALATALLMIVFGTTNFLRDHSASRMVIGAKDFTEQIILGELLAQTIE
                     AKTGLQVERRFDLGGNLAHQAMIGGEIDVYVEYTGTALLAILKKPPIKDAAEAYRQVK
                     SSYAKEFSSEWTEPLGFDNTFAILVRADDARRDKLKTISDAARLSSHWRAGFGQDFMS
                     RPDGYVGFIKTYGLNFKEVREMDLSLTYRALAEGQVDLIAGNSTDGLISRYGLVQLED
                     DLRYFPPYDAVPVVRQKALENHPEVREALKQLGGILSVEEMRDLNYAVDGEQRQAKDV
                     VREFLTRKKILIDR"
     gene            complement(1917..2684)
                     /locus_tag="VF182_11310"
     CDS             complement(1917..2684)
                     /locus_tag="VF182_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015144747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6437711.1"
                     /translation="MTDNRVAIEFDNVSFEIGGGKKLLVDLNLTIDRGETVVLLGRSG
                     SGKTTTMKMINRLLDPTSGEVRVEGTSTGAWDPIKLRRRIGYVIQEIGLFPHLTIEEN
                     IGLVPRLESWPAERIRNRARELLTMVSLEPDRFAARYPAELSGGQRQRVGVARALAAD
                     PPMILLDEPFGALDPITRREIQQEFRSLQKQLGKTMVFVTHDVAEAFALATRIGLLKN
                     GEMIVLGPPAELLASNHPEAKAFVQCFQQSDRAPSDP"
     gene            2743..>3391
                     /locus_tag="VF182_11315"
     CDS             2743..>3391
                     /locus_tag="VF182_11315"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX6437712.1"
                     /translation="METEPLPSILAAQQKARWKTNQDGIWTLDGSRVDEIPARSILTT
                     LWDQPRWLEAYHLYDQRGSELFEQICQLPEYYLTRTENSILEENAARIIAAAPVDCIV
                     ELGAGYSKKTIHLLTEQLRQRDKGIFAPIDVSESGLLASREAVARDFPGLEFQGLHAR
                     YEDGFTSIEQAVPTLFVFLGSTVGNFNHSDFPRFFRALSEAMGPNDYLLLGADRIK"
BASE COUNT          807 a          967 c          852 g          765 t
ORIGIN      
        1 atccatccaa ggttgggaag aacgggaacg acaagagatc ctccctgccc ttataaggcg
       61 gcggcaagaa atccaaaaga gcccgatcat tttcgactac atgtccgtca gcatcgataa
      121 atcccttaac cttagtcatc gcattcctca cttccccgtt tccccggcaa aacgttacgc
      181 aagtactttg cggtcttctc aaccatatca gttatcaaga aaagatgaag atctgcaatg
      241 catggaagaa gctgttcatg ctcctgcgcc acgttgtaaa ttcgactccg atgctttttc
      301 gcagccaggt aacatttttt cctgattaac ggaaagttga agtcgtatac gtcaacgatc
      361 aattaagatc ttcttgcgcg tcaaaaactc gcgaaccaca tccttcgcct gtcgctgttc
      421 gccatcgact gcatagttga ggtcacgcat ctcctcgacg cttagaattc cgcccaactg
      481 ttttaaggct tctcttactt caggatgatt ctccaaagcc ttctgcctca cgaccggtac
      541 ggcatcataa ggaggaaagt agcgcagatc gtcctcgagc tggaccaggc cgtagcggct
      601 gatcaggcca tcggtcgaat ttccggcaat caaatcgact tgcccctcgg ccagagcgcg
      661 ataagtcaaa gacagatcca tttcacgtac ttccttgaaa ttaagcccgt aggtttttat
      721 gaagccgaca taaccgtcgg gccgtgacat gaaatcctga ccaaagccgg cgcgccagtg
      781 ggacgacaat cttgcagcgt cactgatcgt ttttagtttg tcgcgccgcg cgtcatcggc
      841 tcgaaccaga atcgcaaagg tattgtcgaa accgagcggt tcggtccatt cgctgctgaa
      901 ctccttggcg taagacgact tgacctgacg gtatgcttcc gccgcatctt taataggcgg
      961 tttttttaag atcgccagca gcgcagtgcc ggtatattcg acataaacat cgatctcgcc
     1021 gccgatcatt gcttggtgag ccagatttcc ccccaagtcg aaacgtcgct ccacttgcag
     1081 gccggttttc gcttcgattg tctgtgccag caactcgccc agaatgatct gttcggtgaa
     1141 atctttcgcg ccaatgacca tgcggctggc actgtgatct cgcaaaaagt tggtggtgcc
     1201 gaacacgatc atcaggagcg cggttgccag cgccggccat acaaacttac ggagtcgact
     1261 tgccgcgccg cgagaacggg ttgctcgttc caaagatcca agaaacagat cggcaatcaa
     1321 tgcaatcaat gcagccggaa ccgctcccgc cagaatcagg acgttgtcat tggctctcaa
     1381 gccgcggaaa atatagcgtc ccaaaccgcc ggcatcgatt gccgctgcaa tggttgcggt
     1441 gcccacgcag ataactgtcg cgacgcgcac gccggcgata atgacgctgc gagctaatgg
     1501 cagttcgacc tgaaggagca gctggcgatc tgtcatgccc ataccgcggc cagcctccct
     1561 aatagccgga tcgacgccac tgattccggt aaaggtattt ctaatgatcg gcagcagtga
     1621 atataaaacc agcgcggcaa tggcggtacg ggcgccgata ccaccgatga tgcggatatc
     1681 gaagagcatc aggttgaccg ggatcagaaa gccaaaaagc gccagactcg gaacggtttg
     1741 catgatgttg gcgaacccca aaaccggttt gctcaatgcc ggtttgcgcg taaggagaat
     1801 gccgagtggc acgccgatca ggagcgcaat ggctatcgaa acgccgacta gaaacagatg
     1861 ctcaaggatc aatgcaacaa cttcagcccg gtgttctagg aaaaagcgca gcaagctcat
     1921 ggatcgctcg gcgctctgtc ggattgctga aaacactgga cgaaagcctt ggcctcagga
     1981 tgatttgaag caagaagctc ggcaggcggt cccaatacaa tcatctcgcc attcttgagt
     2041 aatccgatgc gcgttgccag cgcgaaagct tcagccacat cgtgagttac gaaaaccatg
     2101 gtcttgccca attgtttttg cagcgagcga aactcttgct ggatttcgcg tcgtgtaatc
     2161 gggtccaaag caccgaaggg ttcatccagg aggatcatcg gcggatctgc cgccaacgca
     2221 cgcgccaccc cgaccctttg ccgctgtcca cccgaaagtt ccgccggata acgcgctgcg
     2281 aacctatcag gctctaagct caccattgtc agcaactctc gagcgcggtt tctgattctc
     2341 tcggccggcc agctttcgag cctcggcact aggccgatat tttcttcaat tgtcagatga
     2401 ggaaataatc cgatttcctg aataacatag ccaatccgtc gccgtagttt gataggatcc
     2461 catgctccgg ttgaggttcc ctcgacccgc acctcaccgg aagtcggatc cagtagacgg
     2521 ttgatcatct tcatggtagt tgttttgccc gagccgctac ggccgagaag cacgactgtt
     2581 tcgccccggt ctatcgtaag gttgagatca acgagaagtt ttttcccgcc gccgatctca
     2641 aagctgacgt tgtcgaattc gatagctact cgattgtcgg tcatcgctcc atcgtctccc
     2701 ccgggtcgca tccgattcca ctttcttata ttctatatcg atatggaaac agagccgttg
     2761 ccgtcgatcc ttgcagcgca acagaaggcc cgctggaaaa ccaatcagga tggtatctgg
     2821 accctagacg gttcccgcgt ggacgaaatc ccggcccgct cgattctcac aactttgtgg
     2881 gatcagccgc gctggctgga ggcctatcac ttatacgatc aacgaggctc cgagcttttc
     2941 gagcagattt gccagctgcc ggaatattat ctgactcgta ccgaaaatag catcctagaa
     3001 gaaaatgccg cccggatcat tgccgccgcg ccagtagact gcatcgtcga actaggcgcc
     3061 ggctacagca agaaaacgat acacttgctc acggaacagc tgcggcagcg cgacaaaggc
     3121 atcttcgcgc ccattgacgt cagcgaatcc ggactgctcg cctcgcggga agccgtcgcc
     3181 cgggattttc caggattaga atttcaagga cttcacgcgc gctacgagga cggctttacc
     3241 agcatcgagc aagccgtgcc gactctattt gtatttctcg gcagcactgt gggtaacttc
     3301 aaccattctg attttccacg tttctttcgc gcattatcgg aggctatggg accgaacgat
     3361 tatctcctgc tcggcgcgga ccggatcaag g
//
LOCUS       DASUVS010000202         4561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1612086, whole genome shotgun sequence.
ACCESSION   DASUVS010000202 DASUVS010000000
VERSION     DASUVS010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612086"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1236)
                     /locus_tag="VF182_11320"
     CDS             complement(34..1236)
                     /locus_tag="VF182_11320"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX6437713.1"
                     /translation="MPVPENSDSRNLPAEIMELQMGPSHPATHGTIKFNLKLEGEKVL
                     DCDVEVGYLHRGFAKSCENVTWTQVIPYTDRLNYASPLINNVGYALAVEKLIGVETPP
                     RCQYIRVIVSELSRIFDHLTCCGMCCSELGAITVGFYMIEAREMITRIVEALTGARLT
                     VTYVRVGGVKHDLPLDFAERVERTFKIIRKLLDDCDGLLSRNRIFIDRMSNIGVISRE
                     TAISYSLTGPILRATGVNYDVRKTNPYLVYDQLDFTVPLGTKGDNYDRFVCRIQEIEQ
                     SMKIVEQALKNLPVGPVWIEDPRFVLPEKEKVYNSIEGLMHHFKIIMEGIKVPPGETY
                     FAVEGGNGELGFYIVSDGAGRPYRVRVRPPCFLAMGAFHEMIKGYMIADIVPTFGMIN
                     MIGGECDR"
     gene            complement(1239..1802)
                     /locus_tag="VF182_11325"
     CDS             complement(1239..1802)
                     /locus_tag="VF182_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX6437714.1"
                     /translation="MPESSPSLQDIQEKLGSKVLETSQFRGDDIVVLDPVDLRESFRI
                     LKEDPGLGFDFLSDITAVDYWKKKEPRFELVYQLLCLQNGKRLRVRVPVSEANAAIES
                     LTPLWRGANFLEREIWDMFGIKFIDHPDLRRILLFDEFKGHPLRKDYPVNQQQHRVPE
                     RKVEGTFVDERSHNKLLRLKKTLGSKD"
     gene            complement(1809..2165)
                     /gene="ndhC"
                     /locus_tag="VF182_11330"
     CDS             complement(1809..2165)
                     /gene="ndhC"
                     /locus_tag="VF182_11330"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX6437715.1"
                     /translation="MDHPYFPLVIVFALAGVVVLALLFIAQKVGPKSTNPAKMEPFES
                     GNPPKGDARIRFSVRFYLIAMLFLIFDLEVVFLYPWAIYFRQLGLFGLVQMGIFLTIL
                     TIGYVYVWKKGALEWD"
     gene            complement(2270..3103)
                     /locus_tag="VF182_11335"
     CDS             complement(2270..3103)
                     /locus_tag="VF182_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX6437716.1"
                     /translation="MTAIVYNGTLRCLRRQSRSNAKRGSKAVMLRWCFVAVGAWFVLN
                     WIYQVVRKPGEILAPVSDSLAKSPDLTWQSYGSLFEKNSTKVISAEFLAALAQVEGSG
                     NPIASTYWRWRWSWNPFEIYRPASSAVGMFQITDGTFAEARKYCIRDHQVFTEGPWHD
                     VNSCWFNSFYSRTVPSHSIQLTAAYLHRTVIDTLAARPRPRSTPAQQQRLAAVIHLCG
                     LKHGQLFAKRGFKLAPGERCGSHNVAQYLARIDTMKRRFVELRRQSADQSTRFPLSAL
                     S"
     gene            complement(3573..4058)
                     /locus_tag="VF182_11340"
     CDS             complement(3573..4058)
                     /locus_tag="VF182_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437717.1"
                     /translation="MADDILIDCGGDYANAAVRSVLENYLPSDVLGAFDGRLAFVSTG
                     DAGGVRLTKSFCRDRDVIVLSERILPVKSGDEEFHPGYRYFIFVVLREIARACKDHLS
                     PLSDDLTAAQLETQTREADELALNWFNEHASTTLFQPPITIAEVEEFRETVRARRDNN
                     K"
     gene            complement(4058..4459)
                     /locus_tag="VF182_11345"
     CDS             complement(4058..4459)
                     /locus_tag="VF182_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437718.1"
                     /translation="MGTIFLLIGFISANVHLAEAQQPPKIPRIGYISGTGDAYHQGPY
                     VEALRQGLKEQGYTESKNIVIEYRGAEAKLDLVGNLVVELVQLKVDVLVLPILPAILA
                     AKKATKRSRDTDRAVSTSPLDHSRRFVYKPK"
BASE COUNT         1125 a         1239 c         1173 g         1024 t
ORIGIN      
        1 caaagatcgc gatgacacga ttctactttg acgctaccga tcgcattccc ccccgatcat
       61 attgatcatg ccgaaagtcg gcacgatatc ggctatcatg tatcccttaa tcatctcatg
      121 aaaagcgccc atcgccagga aacagggcgg ccgcacacgc acccggtagg gccgtccggc
      181 gccatcgctg acaatataaa agcccagttc tccattgccg ccttcgaccg caaagtaagt
      241 ttcacccggc ggcaccttta ttccttccat gatgatttta aaatgatgca tcaatccttc
      301 gatgctgttg taaacttttt ctttctccgg cagcacgaaa cgaggatctt cgatccacac
      361 cgggccgacg ggcaaattct tcagcgcctg ctcgacgatt ttcatgctct gttcgatttc
      421 ctggatgcgg caaacgaacc gatcgtagtt gtcgcctttg gttcccagag gcacggtgaa
      481 atcgagctgg tcgtaaacca aatagggatt ggttttccgg acatcataat tgacgccggt
      541 ggcgcgcaag atcggaccgg tcaggctata gctgatggcc gtctcccgcg aaattacacc
      601 gatattggac atacgatcga tgaagatacg gttgcgcgac aggagtccgt cgcaatcgtc
      661 caacagcttg cgaatgatct tgaaggtgcg ctcgacccgc tcggcaaaat ccagcggcaa
      721 gtcgtgcttg accccgccaa cgcggacgta agtcaccgtg agccgggcgc cggtgagagc
      781 ctcaacaatt cgcgtgatca tttcgcgggc ttcaatcatg tagaagccga cggtaatcgc
      841 gccaagctcg gagcaacaca tgccacagca agtaagatga tcgaaaatac gcgagagctc
      901 gctgacgatc acgcgaatat actggcagcg cggcggcgtc tcgacgccga tcagcttctc
      961 taccgccaag gcataaccga cattgttgat caacggcgac gcatagttca gccggtcggt
     1021 ataaggaatt acctgggtcc aggtgacgtt ctcgcagctt tttgcaaagc cgcgatggag
     1081 atagccgact tcgacatcgc aatcgagaac cttctcacct tccagcttga gattgaattt
     1141 gatggtgcca tgggttgccg gatgggatgg gcccatctgc aattccatta tttctgccgg
     1201 cagattccgg ctgtcggagt tttccggcac tggcatggtt agtctttgct ccccaacgtt
     1261 ttcttcaaac gcaaaagctt gttgtgcgaa cgttcatcga caaaggtgcc ttcgaccttg
     1321 cgctcgggga cccggtgctg ttgttgattg accggataat ccttgcgcaa cggatgaccc
     1381 ttaaattcat caaacagcaa aatccggcgc agatccggat gatcgataaa tttgatgcca
     1441 aacatgtccc aaatttcacg ctccagaaaa ttagcgccgc gccaaagcgg cgtcagcgat
     1501 tcgatagcgg cattcgcctc ggataccggc acccgcaccc gcaatcgttt gccgttttgc
     1561 aaacaaagca attggtaaac gagttcgaag cgtggttctt tctttttcca gtagtcaacg
     1621 gcggtgatgt cagaaagaaa gtcaaaaccg agcccaggat cttcttttag tatacgaaaa
     1681 ctctctctca aatccaccgg gtcgagaacc acgatatcgt cgccacgaaa ctggctcgtc
     1741 tccagaacct ttgagccgag cttttcctgg atgtcctgaa ggcttgggct gctctcaggc
     1801 atcggcgatc aatcccattc cagcgcgcct tttttccaca cgtacacata accaatagta
     1861 aggatggtga ggaaaattcc catttgcact aatccaaaca gccccagctg tcggaaatag
     1921 atcgcccacg gatagagaaa aacaacttcc agatcgaaga ttaaaaacag catggcaatc
     1981 aaataaaacc gcaccgaaaa acgaatacgc gcgtcgcctt tgggcggatt accggactca
     2041 aacggttcca tctttgccgg attggtggat ttcggtccaa ccttttgcgc aataaagagc
     2101 agagccagaa ccacaactcc cgccagcgca aatacaatga ccagaggaaa ataaggatga
     2161 tccatgcagc tatctaaaaa aaaccatacc taaccgtttc cagaggtatg gattggtaga
     2221 ataaatgcta gcggtactta aaaatttcta ttggaaagtt gcggggttgt caagataacg
     2281 ctgacagcgg gaatctagtc gattgatcgg ctgactgcct tctcaattca acgaaccgtc
     2341 gcttcatcgt atcgatgcga gcgagatatt gggccacatt gtgagaccca cagcgctcgc
     2401 cgggcgctaa tttgaagccg cgctttgcga agagttgccc gtgtttcaac ccgcataggt
     2461 gaatcaccgc ggccaatctt tgctgttgag ccggagtgct ccgagggcgg ggccgagcgg
     2521 cgagcgtgtc gatcacagtg cggtgcagat atgcggcggt tagttgaatc gaatggctcg
     2581 gcacagtgcg gctatagaag ctgttgaacc agcaggaatt gacgtcatgc cacggtccct
     2641 cagtaaagac ctgatgatcc cggatacagt actttcgcgc ttcggcgaac gtcccgtcgg
     2701 tgatttgaaa catgccgacc gcgctcgaag ccggacgata aatctcgaaa ggattccagg
     2761 accagcgcca gcgccaatag gtgctagcga tgggattgcc gctgccctcc acctgagcca
     2821 gcgcggcaag aaactcagcc gaaataacct tcgtagaatt tttctcgaat agggatccat
     2881 agctctgcca ggtcaaatcc ggcgacttag ccaaggagtc actgactggc gccaggatct
     2941 ccccgggctt acgaaccacc tgatagatcc aattgagcac gaaccaagct ccaactgcga
     3001 caaaacacca gcggagcatg accgccttcg atccccgctt ggcgttactc cgagactgtc
     3061 gcctgagaca gcgcaaagtg ccgttataga caatcgccgt cacgttgaag gacacctctt
     3121 agcattcgca ctgacatcat ccgacatcga ttatacttcg gctcgagatc tggaactagc
     3181 aacgaacttg attttgagca gggttgaatt tggttcagtt gattcagtgg tttgaacggt
     3241 cgaaccctga ttccgcggta gacatcgaca atgtgtggca ataaagggtg agcctggagg
     3301 agcgaaggca tagaagcgaa acttcaatgt cgtccagggt gcttggtcca gggctgacaa
     3361 tctttgcccg agtccgctgc agcccgaaga ttgcaagctg ctacaagctg ctggcctgtt
     3421 gcattcgctc tgcaggtttg ccgctatcgc tgtgtcacgt tggattcggg agtccggttg
     3481 aactgccaac ggcttattcg cagtatagga ccgatgaacc ctggtcatga atccacgact
     3541 tccatagtta acgcgtatcg gctgacggct tatcatttat tgttgtcacg ccgtgctcgg
     3601 accgtttccc tgaactcctc aacctcggca atcgttatag gcggctggaa cagagtggtc
     3661 gaggcgtgct cgttgaacca attcaacgcg agctcatcag cctcgcgcgt ttgagtctcg
     3721 agttgcgccg ccgtcagatc atccgataac ggcgagaggt ggtccttgca agcgcgcgct
     3781 atctcacgaa gaaccacgaa gataaagtaa cgatagccag ggtgaaactc ctcgtcaccc
     3841 gacttcaccg gcaggatgcg ctcggacaac acgatgacat cacggtccct gcagaagctc
     3901 ttggtcaggc gaaccccgcc cgcatcaccc gtggagacga aagccagccg gccgtcaaac
     3961 gcgccgagca cgtcagaagg caggtagttc tccagcacgg aacggaccgc ggcattggcg
     4021 taatcgccgc cgcaatcaat cagaatgtcg tcagccatta cttcggcttg tagacgaacc
     4081 tacgggagtg gtcaagggga gatgtagaaa cggctctgtc tgtatctcgg gatcgttttg
     4141 tcgccttctt tgctgcgagt atggctggca ggatggggag gacaagcaca tcgactttga
     4201 gttgcactaa ttcaactaca agatttccga caaggtccag ctttgcttcc gcgccgcgat
     4261 actcgatgac gatattttta ctctctgtat agccctgttc cttgagacct tgtcgcaatg
     4321 cctcaacgta aggcccttgg tggtaagcat cgcccgtccc tgaaatatag ccgatgcgtg
     4381 gaattttagg tggctgctga gcctcagcga gatggacatt agccgatata aaaccaatca
     4441 gcagaaagat agttcccatt gacggatttt taccgctgtt cgatatcggt tgtccacaaa
     4501 gttttagaga tcaacgcgat cgcggtaatc cataagggga atcgggtgaa accccctgcc
     4561 c
//
LOCUS       DASUVS010000203         7706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1394048, whole genome shotgun sequence.
ACCESSION   DASUVS010000203 DASUVS010000000
VERSION     DASUVS010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7706
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394048"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VF182_11350"
     CDS             complement(<1..303)
                     /locus_tag="VF182_11350"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX6437719.1"
                     /translation="MAIYTPLAKKDAIKIADEFALGDLVSITGIKNGSVNTHYLLETK
                     RGRFFAKIDEIKGELEVKQELDLLFYLKKQGFPCLQPIKSKTGRHYVEIDGKILTIS"
     gene            851..1051
                     /locus_tag="VF182_11355"
     CDS             851..1051
                     /locus_tag="VF182_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_033015550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX6437720.1"
                     /translation="MATGKVKWFNNSKGYGFIEKEGGGDVFVHYTAIQGDGFKTLNEG
                     QLVEFELSQGDKGPQATNVTKV"
     gene            1186..1692
                     /locus_tag="VF182_11360"
     CDS             1186..1692
                     /locus_tag="VF182_11360"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEX6437721.1"
                     /translation="MDTVNAVKDSKFGQDQLLNETVVLTRLDKAIGWARKYSLFPYPF
                     ATACCAMEYMSLSMSPYDIDRFGALLPRFSPRQADLLMVIGTVNHKLSPVLKRVYEQM
                     SEPKWVMAFGACAASGGFYDNYATVQGIDRIIPVDVYVPGCPPRPEAVLDGLMELQKR
                     ISNAKQPV"
     gene            1689..1997
                     /locus_tag="VF182_11365"
     CDS             1689..1997
                     /locus_tag="VF182_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437722.1"
                     /translation="MRDEETAILPHTDDLFHHGVITKLFPSNNTGIVRIESGREVFFS
                     YEFVILTGEVKSPLNLKAGQPVGYDLGWTPSGLRVTKIKTYPAAAGIRSPGTSKGQGS
                     "
     gene            complement(1977..2894)
                     /locus_tag="VF182_11370"
     CDS             complement(1977..2894)
                     /locus_tag="VF182_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437723.1"
                     /translation="MWLRAGYVAAVSIRRGVPSCGKQAAPKIGMRGFSSSFLLFFYQI
                     ALGGLFGLAFTPFNELDRAFYKSTGGVLFVIALLGFWGKTNLYLHSISKSFSWIVAAD
                     ALAHFLFVVFFACYIVSLWGERRLFRARTFAACIFSGLAGIFLSAHSFYEAPIGSIET
                     LVYPLSFFLSCLLLGSVTVGMLIGHWYLIDTGQTLDPFIRIYKFFVISLVVQSCFSPV
                     SFLLLYFLGTDQSIVELEKLWAHHSTLLMTRVLIGYLGPLALSWMIWRTLLIPHTMAA
                     TGLFYIALLGVFVGEILSKQLLALASLPF"
     gene            complement(2810..3532)
                     /locus_tag="VF182_11375"
     CDS             complement(2810..3532)
                     /locus_tag="VF182_11375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HEX6437724.1"
                     /translation="MTSIDNPGNENVRRMFDRIAGRYDLLNRVISFRLDAWWRKKAVS
                     AVVRSGYEKILDLGTGTGDLTFAASKSLRGHGRVIGLDFSFKMIQLARVKQESQNSST
                     TEFLQANALFPPFKDEVFDGVMTGFVLRNVSDLRIFFIQAFRVLKPGGRIASLDMFPP
                     AAGIFATFYAFYFYRFMPWIAGLLAGDRKAYQYLSDSVRQFVPPDRVSQLLEQCGFEQ
                     VTLQRFLFGAVCLHVANKPHQK"
     gene            complement(3588..4628)
                     /locus_tag="VF182_11380"
     CDS             complement(3588..4628)
                     /locus_tag="VF182_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX6437725.1"
                     /translation="MIHETKSKTVKSADSFTSLGFRIPSLQGSSDPFRYIAQELQLVE
                     ETLARAIRSREQSLTDIAAHIILGGGKRVRPAVTILAFLAFGGKRASDIVDIAAAMEL
                     IHTATLLHDDIIDGAETRRGKDSAYKKFGLKSTLVAGDFLFIKAFEFAGKFDETVVQW
                     TADACSLLTEGEMLQGHFNRNRDVTLDDYIEIVKRKTASLFHTGGKVGAYLAGADANH
                     VEATECYGLNMGIAFQVVDDILDIVGHVELLGKPTGMDLRDGNPSLPIILALQDRESA
                     VCEAFECEQPSEGQIAAALDALKTGNAIEAAKSISKRYAEDALKSIKKFPPSLYRNGL
                     KNLVQLIIDRDF"
     gene            4902..5597
                     /locus_tag="VF182_11385"
     CDS             4902..5597
                     /locus_tag="VF182_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEX6437726.1"
                     /translation="MEAAHFFFRWFHFLAGITWIGILYYFNFVQTPFFAETEASVRTG
                     AIQKLVPRALWWFRWGAMITFLAGLLMYLMRLGEMGAGLFFTSSYGVTISLGALMGTI
                     MFLNVWLIIWPNQQIVIASTNQVASGGQALPAAAGAARRGALASRTNVVFSIPMLFFM
                     GAASHYPSLVGPSAAFGVFWILALIIIAAVEINPLVGTQGATKKFLDSVSGALWAGFI
                     LTAIFYVLLLALM"
     gene            complement(5606..5947)
                     /locus_tag="VF182_11390"
     CDS             complement(5606..5947)
                     /locus_tag="VF182_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6437727.1"
                     /translation="MANAFIKLVIVLIAVGLSACSKDSDQAAQASKDPDWERGRSVYI
                     ANCVACHNNDPSKDGPIGPALKGSDRELIEARVLTSKYPPNYKPKRPTQVMPQFPFLK
                     SEISYLAAYLR"
     gene            complement(6151..6237)
                     /locus_tag="VF182_11395"
     tRNA            complement(6151..6237)
                     /locus_tag="VF182_11395"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6201..6203),aa:Leu,seq:cag)
     gene            6669..6848
                     /locus_tag="VF182_11400"
     CDS             6669..6848
                     /locus_tag="VF182_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437728.1"
                     /translation="MLNEKAKARDIATGAAPAPKRGQVIDIREALKRSMESGGEQTGD
                     AFAAKKIKNVVGVGN"
     gene            7367..>7706
                     /locus_tag="VF182_11405"
     CDS             7367..>7706
                     /locus_tag="VF182_11405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEX6437729.1"
                     /translation="MDPFQRLVTSNLEAWQNFRTRHPILFNSMYPAYRLYKQGRRWKY
                     RQIYNRFKDYTMLLPDWYAANLELCENFGHIAGNIVECGVWKGGMSAGIAYLLGDDRA
                     YYLYDSFEGLP"
BASE COUNT         1934 a         1892 c         1934 g         1946 t
ORIGIN      
        1 gctaatggta agtattttgc catcaatctc cacgtagtga cggcccgtct tactcttgat
       61 gggctgcaaa caaggaaacc cctgtttttt caagtagaaa agcaagtcaa gttcctgctt
      121 aacttccagt tctcccttga tttcgtctat ttttgcgaaa aatcgcccac gcttggtctc
      181 gagaagatag tgcgtgttca cggagccatt tttaattccg gtaatactca caagatcgcc
      241 aagtgcaaac tcatcggcga tctttatagc gtcttttttt gccagtggag tataaatagc
      301 catctgttaa gttaccgact gatttcaggt ttttaacgga atatcccgtc cagatctgct
      361 aaccccgcta aatataatga aaaacgctga tcgtgtcaat atctaagtgc ctcagtcaag
      421 taaatgaggt gtactgcatt cgaggttgtt gagagcttag ttttaccggc tgaaaaccga
      481 caccgcgacg ccgggatcgc cattttcctc atcgaggata agctttcaac ggtcaaaatg
      541 tccatacgga gtcgcacacg agctgtttca tcacctttgc gtgagttgct ggtttgagcc
      601 gatgagcaat tgagaaacct tttgttacac agctatgtgg gaaaactttt ctcttgacaa
      661 ttctacatac gtttttttaa tgtctctgac gtactatgga tcgcacgtca gtaatgggac
      721 accagaccgg cacgattatt tgctagagcg tgcttgggtt agtaagagac aggaatcgtc
      781 gtcggcagag atcgtccgag ttgagtccgc atgctatagc cgggcaacgt ggacaggagg
      841 tgttagggga atggcaacag gaaaggtaaa gtggttcaat aattcgaaag gatacggttt
      901 cattgagaaa gaaggaggcg gcgatgtgtt tgtccattat accgccattc agggcgatgg
      961 gtttaaaact cttaacgagg ggcagctagt cgaattcgaa ctatcacaag gcgataaagg
     1021 ccctcaggcc accaatgtca caaaagttta acggcaacga catctttggg acaatacgaa
     1081 ctcgccgcca attgacgggc catgcttgca gctcgcttgg tgagggagag tttgcagttt
     1141 ggagttgggc cgcgctgttt atttaaaggc acatagatca tacaaatgga tactgtgaat
     1201 gcggtgaagg attcgaagtt cgggcaagat caattattga atgaaacagt agtgctaacc
     1261 cgtttggata aggcgatcgg ttgggcacgg aagtattcct tattcccgta cccgtttgca
     1321 acggcatgtt gcgcgatgga gtacatgtca ctatcgatgt cgccctacga tatcgaccgt
     1381 ttcggagcgc ttttgccgcg ctttagtcca cggcaagcgg atctgcttat ggtgattggc
     1441 actgtcaatc ataagttgtc gcccgtttta aagcgtgtct acgaacaaat gtccgaaccc
     1501 aaatgggtca tggcctttgg tgcatgcgca gcgagcggcg gtttctatga taattacgcc
     1561 acggtgcaag gaattgatcg catcattcca gtggacgttt atgtcccggg ctgcccgccg
     1621 cggcccgaag cagttcttga tggcctgatg gagttgcaga agcgcatttc gaatgcaaag
     1681 cagccagtat gagagatgaa gagaccgcca tcctgccgca caccgacgac ttgttccacc
     1741 acggcgtcat cactaaacta ttcccctcaa ataataccgg tattgtacga atcgagagcg
     1801 ggcgcgaggt tttcttttcg tacgagttcg tgattcttac cggggaagtt aaatcccccc
     1861 tgaatctaaa ggctggacag ccggtcggtt acgacttggg ctggactcct agcgggcttc
     1921 gagtgaccaa aataaagact tatccggcgg cagcgggcat ccgatcgccg ggcacttcaa
     1981 aagggcaggg aagctagggc cagtagttgt ttgctcaaga tctctccgac aaacacaccc
     2041 agcagcgcaa tgtagaataa ccctgttgcc gccatcgtgt gcggaatcag caaagttcgc
     2101 cagatcatcc aagagagagc caatgggcct aggtaaccga tcaggacccg tgtcatcagc
     2161 agggtcgagt gatgggccca cagtttttca agctccacaa tactttggtc ggttccgaga
     2221 aagtacagca acaaaaaaga gaccggtgaa aagcaactct ggaccacgag ggagataaca
     2281 aaaaatttat agatgcggat gaacggatca agtgtctggc cggtatcgat caagtaccaa
     2341 tgcccgataa gcatgcccac cgtcacgctg ccaagtaaaa gacacgacag aaagaacgac
     2401 aaggggtaaa ccaaagtttc aattgaccca atcggcgcct cgtaaaagct gtgcgcgctc
     2461 aagaaaattc cagccagacc tgaaaaaatg cacgctgcga atgttctagc tctaaaaagt
     2521 cgtcgttcac cccaaagcga gacgatatag caggcaaaaa aaacgacgaa gaggaaatgg
     2581 gccagagcgt ccgcagccac gatccagcta aagctcttcg atatgctatg taaatagagg
     2641 tttgtttttc cccagaaacc tagcagggca attacaaaca gtacgccgcc ggtcgatttg
     2701 taaaatgctc gatcgagttc gttgaatggg gtaaatgcca atccaaagag gccgccaaga
     2761 gcaatctgat agaaaaaaag taaaaagcta ctggagaagc ctcgcatgcc tatttttggt
     2821 gcggcttgtt tgccacatga aggcacaccg cgccgaatag aaaccgctgc aacgtaacct
     2881 gctcgaagcc acattgttca agtaattgtg aaactctatc cggaggcaca aattggcgca
     2941 ccgagtctga caggtactga tacgcttttc gatccccggc gagaagtccg gcaatccaag
     3001 gcataaagcg ataaaaatag aaggcataga atgtcgcgaa tatgcctgcc gccggcggaa
     3061 acatatccag cgaagcgatc ctgccgccgg gttttaagac ccggaaagcc tgaataaaga
     3121 atatccgcag atcagaaaca ttacgcagta cgaacccggt catgacgcca tcgaacacct
     3181 cgtctttaaa tggcgggaag agtgcgtttg cctgcaggaa ctctgttgtg ctgctgtttt
     3241 gactttcttg cttgacccgg gccagctgta tcatcttgaa ggaaaaatcc aatccgatga
     3301 ctctgccgtg gccgcggagc gatttgcttg cagcaaaggt gaggtctcct gttccagttc
     3361 ccagatcaag gattttttca taaccgcttc taacaaccgc ggaaaccgcc tttttcctcc
     3421 accaggcatc caagcggaag ctaataaccc gattgagtag atcgtaacgg ccggcaatgc
     3481 gatcgaacat gcgccgtaca ttttcattcc ccggattatc gatggatgtc atctggtgat
     3541 ggtaaccgta tcatcgtagc tgcgaatgtt tgagccagcg gcgacgatca aaaatctcga
     3601 tcgatgatga gttggacgag gttcttcaat ccattgcgat aaagagacgg agggaacttc
     3661 ttgatggatt ttagcgcatc ttcagcatag cgcttggaaa tgctcttggc tgcttctata
     3721 gcgtttccgg ttttcagagc atcgagagcc gcggcgattt gaccttcact cggctgctcg
     3781 cattcgaaag cctcacaaac ggcgctctcc cggtcctgca aagcaaggat aatcggcaaa
     3841 gacggattgc catcacgcaa atccatcccc gtcggttttc ctaacaactc gacatggcca
     3901 acaatgtcaa gaatgtcgtc cacaacctgg aatgcaatcc ccatgttgag gccgtagcat
     3961 tcggttgcct caacatgatt tgcatcggcg ccagccagat aagcgccgac tttacctcca
     4021 gtatgaaaga gcgatgccgt ttttcttttg acgatttcta tatagtcgtc gagagtgaca
     4081 tcccggttgc gattgaaatg accctgcagc atctcacctt cggtcagcag cgagcaggca
     4141 tcggctgtcc attgcaccac cgtctcatca aacttcccgg caaattcaaa agctttgata
     4201 aaaagaaaat caccggcgac caacgtagat ttaagcccaa attttttata agcggagtct
     4261 tttccacgcc gggtctcagc gccatcgatg atatcatcgt gaagtagagt tgccgtatgg
     4321 ataagttcca tggcggcagc aatatcgacg atatcgctgg cccttttacc tccgaacgct
     4381 aaaaaagcca gaatcgtcac ggccggacgc acgcgcttac cgccgcccag aattatgtga
     4441 gctgcaatat cagtaagtga ttgctcccga gaccgaatcg cccgcgccaa agtctcctcg
     4501 actagttgaa gttcttgggc gatatagcga aacgggtcgc tgctgccctg tagcgaagga
     4561 attctgaagc ctaaactcgt aaagctatcc gcggatttta ccgtcttact ttttgtctcg
     4621 tggatcaaaa acactccgtg cggtgaagga gagagagtta gcaggaaaat gtcatagatc
     4681 aataatcagg tcaagccgag tcggagtcgt tttcgactca tatcggttca tccggtttac
     4741 cgaaaaagtc catggtttgt cctttccagg tcgagttcta cgaacgatca catttcgcat
     4801 cgagaaagct cgacgtttgc cttgacaaat tcactgctgc cctaatatat aaaaacctgc
     4861 tttttgatag attgaattag gattaggagg aggggagttc tatggaagcg gcacattttt
     4921 tctttcgatg gtttcatttt ttagccggga tcacctggat cggtatcttg tactatttca
     4981 attttgtcca gacacctttt tttgcagaaa ctgaagcaag cgttcggaca ggagccattc
     5041 agaagcttgt gcctcgcgcc ctgtggtggt ttcgttgggg ggcgatgatc acgttcctgg
     5101 ccggtttact gatgtacttg atgcgacttg gcgaaatggg tgcgggacta ttcttcacct
     5161 cgtcgtatgg ggtgaccatt agtctgggcg ctttaatggg gacgatcatg tttctcaatg
     5221 tctggttgat catttggccc aatcagcaga tcgtcatcgc ctccaccaac caagtcgcca
     5281 gcgggggaca ggcattgccg gcggccgccg gggcggcgcg acgaggggct ctggcatcaa
     5341 ggaccaacgt ggtattttct attccgatgc tcttttttat gggcgctgcg agtcactacc
     5401 cgtcgttggt cggaccctcc gctgcattcg gcgtgttctg gattttggcg ctgatcatta
     5461 tcgccgcagt ggagatcaac ccgctggtgg ggacacaagg agcaactaag aaattcttag
     5521 attctgtttc cggcgcgctg tgggctgggt ttattctcac cgctattttt tacgtgttat
     5581 tgcttgccct gatgtgaggg caagctcagc gaaggtaggc tgcaagatac gaaatttctg
     5641 acttcaaaaa cggaaattgc ggcatcacct gggtcgggcg cttcggttta tagttgggcg
     5701 ggtacttact agttagcacc cgggcttcga tcaattcccg atcagaacct ttcagcgcgg
     5761 gtccgatcgg accatctttc gatggatcgt tgttgtgaca tgcgacgcag ttggcgatgt
     5821 aaacggatct tcctctctcc caatcaggat ctttgctggc ctgcgccgct tgatcggagt
     5881 cttttgaaca ggcactcagg cctacagcga ttaagacgat taccaacttt atgaaagcgt
     5941 ttgccatctg aagtctggtc catttgcgcc tacttgcctg actggcccct cagtcgtcga
     6001 cccagcttat ttcgagaact caccacgttc ttaaaccatt gaggacctaa gtattcgtga
     6061 gaatgaggca tcaccgggct agcggcatac tatcgttcga aaaagatgat ttagtattaa
     6121 cagagaaatc ctggcgtgtg ttattcatcc tggtgcgaaa gaggggagtc gaacccctac
     6181 gagtgttacc tcacaggatc ctgaatcctg cgcgtctacc aattccgcca ctttcgcgag
     6241 gcgcggttcc ttttaatatc agacagttaa ctattttctc tgctcgtgtc aatcgcttgc
     6301 ttgcgcctca agcgatccaa tctgtgccta acctcaaatt tgccgccgct cttttagatc
     6361 tcggaactcg atctttccgg ctcgtattct cttgcggcgt ctttcatcag ccttgagtcc
     6421 gtccccaaat tacttaaaca gcgagagggc gtttttttgg gcacatagtg ctcccattta
     6481 gctagttatc agtgaaaagt atgctcgcgt tgatctctga ctctaacaaa ctgtcgaaat
     6541 taaattttac cgcgacgagt ttagaaatca tcatcagtga tgttcggtgt cggcttattt
     6601 gagcgccgac gtccgatgag ttccagcctc aaaattacaa agtttgaata ccgcatccgc
     6661 gtgctcggat gcttaatgag aaggcgaagg ctagagacat cgcgaccggt gcagcaccag
     6721 cgccgaaacg cggtcaggtg atcgacatcc gggaagcact aaagcgcagc atggagagtg
     6781 gcggcgaaca aaccggcgac gccttcgcag ctaagaaaat aaaaaatgtc gtaggcgtcg
     6841 gaaactaacg gccctcgttc aatatgttta atcctgctcg cgcaacatac cgggcgccgt
     6901 gaggaccgtt gccggatccg ggattgacat cgccggcggt ctgttgttct cgccgcgcca
     6961 accagaagga tcgatgtgaa atgctatact gtacatactt gtccttattg ctagagccga
     7021 gctccccacc ggtagcttgc ctgcgagttc agaaattttg cctttaggaa ccggtgaaga
     7081 ttcagcacaa gctgctcttc aatcggtagc cgagcctcac tttcccgcat attacgctca
     7141 tgctgcgcga tttcggcttg tagttctcac atgcgtttca gcagtgcttt ctgtcgagag
     7201 attttgtgga tttaacataa atgtgcggta gcttatcgct gagtgatcaa ggatcaggtg
     7261 acggactgca ccttttttct tgcgaacctc cgtgcattgc tcgtttgtta tcctcatgct
     7321 aatttttgga ttgttcacga aaattccgac taagtcagga taaagcatgg atccattcca
     7381 gcggctcgtc acgtccaatt tggaagcctg gcaaaatttt aggacacgtc accccattct
     7441 tttcaattcc atgtacccgg cctataggct ttacaagcag ggtaggcgct ggaaataccg
     7501 ccagatctat aaccgcttca aagattacac gatgctactg ccggattggt acgcagccaa
     7561 cctcgaactc tgcgaaaatt tcggccatat cgccggaaac atcgtcgagt gcggagtgtg
     7621 gaaaggcggg atgagcgctg gaattgccta tttgcttggt gacgatcgcg cctattatct
     7681 ctacgatagt tttgaaggat tgccag
//
LOCUS       DASUVS010000204         3922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1176152, whole genome shotgun sequence.
ACCESSION   DASUVS010000204 DASUVS010000000
VERSION     DASUVS010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176152"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..1254)
                     /locus_tag="VF182_11410"
     CDS             complement(247..1254)
                     /locus_tag="VF182_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011749537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6437730.1"
                     /translation="MIIDAHTHYTTAPAQLQAYRGQQITHLGKPIPAKLQISDEVLAR
                     SMEGQFKRMKESGIDRLLFSPQASAMGHHFGSALVSRYWTEACNDLIARVARLWSDKI
                     SPVCQLPQSPDVSPKEWVDELDRCVKELGFVGCNVNPDVAGGREPLTPSVGSEWWYPL
                     WEKMVELDVPCTIHASASLNPAMHLTNSYYLAHHNSAAIELLKSRVFKDFPTLKIIIP
                     HGGGAVPYQWNRNRGMHVKEGLEAFEEAARRVYWDMAIYDKESMELLIRRVGADNVLF
                     ATEMFGAVNAIDPKTGRNFEDLVPIFQEIDWLTNEERYKITEGNVRKLFPRIFDGPRV
                     R"
     gene            1709..1963
                     /locus_tag="VF182_11415"
     CDS             1709..1963
                     /locus_tag="VF182_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437731.1"
                     /translation="MRIQRKMNQALLALAISLLFVPQSHAHGGDPNLIHACINNEGIV
                     RIVAPNDECAKSETARHWIRVRQGDKPPRDREGFGTKLTL"
     gene            2228..3247
                     /locus_tag="VF182_11420"
     CDS             2228..3247
                     /locus_tag="VF182_11420"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX6437732.1"
                     /translation="MIHTRICDLLEIVHPIILGGMGTATTAPLVAAVSNAGGFGTLGT
                     SAFNAATLDKEIASILERTEKPFGINHLLFQIREDMFTVTLNAKPTVAAFAWARKDQD
                     LREYFERAHDVGCRVMYMAGEVPEALRGADAGADVIVAQGTEAGGHVGWMASLPLLPM
                     MVKAVAPLPVLSAGGIADGRGLAAALALGAEGVLLGTRFMATPEAPIHPNFKQAIVES
                     DGHDTVLTEIPDLASQRVWPGAMSRAKRNGFIERWSGREWALRQNAQEIGRQVAAARA
                     AGDIDNASLSFGQDAGLISSIKNVREVIQEIVGEAEAIIKDRLPNLVRQSGELNKLSS
                     DKKTA"
     gene            complement(3664..>3922)
                     /locus_tag="VF182_11425"
     CDS             complement(3664..>3922)
                     /locus_tag="VF182_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437733.1"
                     /translation="CGIRVALSRVVIPQHRIKQRKGRVMRPLKNLRNERGQGLMEYTL
                     IIFLVALVFWGGMKSTNIGDELAGSWDKVIDCITTPFSCGS"
BASE COUNT          889 a         1040 c         1062 g          931 t
ORIGIN      
        1 ctatgatgtc acacttgtag catctttcca atactgagcg cacatgtacc ttttggctcc
       61 tgattaagta catatcccgc gacggctctg ccggcatcgc tccgtggcgc gggcgctgat
      121 gttaacctcg gtggttttat ctcgatagca ggtttcgcgc agaagcagga tgagcttaac
      181 attgtaaact tcttcatagg catccgttgt ttgtctatca aaggtcgccg gcgataggta
      241 gtgcgatcac ctaacacgcg gtccatcgaa aatgcgtgga aagagctttc tcacattacc
      301 ttcggtaatt ttgtatctct cttcattggt gagccaatca atttcctgaa agatcggcac
      361 aaggtcctcg aaatttctgc cggtctttgg gtcgatggca ttaacggcgc cgaacatctc
      421 agtggcaaag aggacattgt ccgcgcctac ccgtctgatt agaagctcca tggattcttt
      481 atcatagatt gccatgtccc agtaaactcg ccgcgccgct tcctcgaacg cctcgagtcc
      541 ttcttttacg tgcatgccgc ggttgcggtt ccattggtac gggacggccc cgccgccgtg
      601 agggattatg atttttaatg ttggaaaatc tttgaacacc ctggatttga gcagctcgat
      661 ggcggccgaa ttgtgatgag caagataata agaatttgta agatgcatcg ccggattaag
      721 cgaagcgctg gcatggattg tacacggcac gtcgagttcg accatctttt cccataaggg
      781 ataccaccac tcactgccga cagaaggcgt caaaggctcg cgcccgccgg caacgtccgg
      841 gttaacattg cagccgacaa atcctaattc tttgacgcaa cgatcgagtt cgtcaaccca
      901 ttccttgggg cttacatccg gcgattgcgg caattggcac acaggagaaa tcttatccga
      961 ccatagtcgc gcaacccggg cgatgagatc gttgcatgcc tccgtccagt agcggcttac
     1021 gagcgccgag ccgaaatgat gtcccatcgc ggaggcctgc ggagaaaata gaagacgatc
     1081 gatacccgat tctttcattc gcttgaactg accttccatt gagcgtgcta gaacctcatc
     1141 gctgatctgc agctttgcgg gtatcggctt tcctaaatgc gtgatctgct ggccgcgata
     1201 cgcctgcagc tgggctgggg ctgtagtgta gtgagtatgg gcatcgatga tcatgagtgg
     1261 tttcctccta tataattgtg gttttctgcc ctgacactct ttgctcgacg atttgcctca
     1321 agcactagtt acaaggagtg cgttattacg ccagacatag cccgcttaga gagcttaatc
     1381 aaagccctcg agcaagccga tagcgctctg gcttcacttc tccagctata ggagaccgtg
     1441 gccggccaag ccggcaagtt cgccgcaccg ccttctagcc tagccacctg tagctgaata
     1501 gcccggcgta ttagcacgac gaattttttt aagctgcttg gatttggctg agagatctga
     1561 ctccacgaag ccgcgttgtt gcgcgcgcgg ccagtcaccc tcgaataccg aagtaaccga
     1621 tatgcagcca cattgacagc caattttcgt gtgatattga gcccggcatt cgggagggtg
     1681 tgatgccgtc ggttgaaagg atgacaacat gagaatacaa cgcaagatga accaggcgct
     1741 gcttgcatta gcgattagcc tgctcttcgt tccgcaatca cacgctcacg gcggcgatcc
     1801 taatctgatt cacgcctgta ttaataatga gggaattgtc agaattgtcg cgcctaatga
     1861 tgaatgcgcc aaatcggaaa cggcccgtca ctggatacgt gttcgacaag gcgacaagcc
     1921 tccccgtgac cgcgaggggt tcggtaccaa gctaacattg taaagcccgc ttctcagggc
     1981 gattgcgccg agagtatgaa gccgagcatt ggacttactc cctaatgcca agcatcgagc
     2041 ggagccgttt cgatctgatc cgcggtgacc ggttcctcgt cgggcgcgga ttccaccttc
     2101 cgggcgccga ttgcaggaat gaaagcgctt catcagagtg acttctaata gctagcgcga
     2161 gttctttatt tacaagggcc ggcatttcaa atagtagagt agcctatcaa ttcgtcatgg
     2221 aggcgccatg attcacaccc gcatttgcga tctgcttgag atcgttcatc cgattatcct
     2281 gggaggcatg ggaaccgcga ccaccgcgcc gcttgttgct gccgtatcca atgccggcgg
     2341 cttcggcact ctggggacgt cggcgttcaa tgcggcaacc ctcgataagg aaatcgcgtc
     2401 gattcttgag cgaaccgaaa agcccttcgg catcaatcat ctgctctttc aaatcagaga
     2461 ggacatgttc acggtgacgc tgaacgctaa accgacagtg gccgcgtttg cttgggcgcg
     2521 caaggatcag gacctgcggg aatattttga gcgcgcgcac gatgttggct gcagggtcat
     2581 gtacatggcg ggtgaagttc ctgaggctct ccgtggcgca gatgcagggg cggatgtgat
     2641 tgtggcgcag ggaacagaag ccggagggca tgtgggttgg atggcatctc tgccgctgct
     2701 gccgatgatg gtcaaagccg tagcgccgtt acccgtgctc tctgcgggcg gtattgccga
     2761 tggccgcggg cttgcggcgg cgctggctct tggggccgaa ggtgtactgc tcggcacccg
     2821 tttcatggcc acgccggaag cgccaatcca tcccaacttc aaacaagcga tcgttgaaag
     2881 cgatggccat gacacagtgc tgacggaaat tcctgattta gcaagtcagc gcgtctggcc
     2941 cggggcgatg tcgcgagcca agcgcaacgg tttcatcgag cgttggtcag gacgcgaatg
     3001 ggcgttgcgg caaaatgctc aggagatcgg taggcaggtc gcggctgcac gcgctgccgg
     3061 cgatatcgac aatgcctcgc tttctttcgg tcaggatgcc ggcttgatta gttccatcaa
     3121 aaatgtccgg gaagtaattc aagagattgt cggggaagcg gaggctatca tcaaagaccg
     3181 cttgcccaac cttgtgcgcc agagcggaga actgaacaag ttatccagcg acaaaaagac
     3241 ggcgtgaaag gttgatggga actgccgatt tgcccggccg ttatcggatt aggcggaagc
     3301 agtaaagaac agttgttggg gcaaaaaatc tcgaccatct ccgctctatt cgacgaggct
     3361 ggcttaatgc gctcgaatta gcgaatgggg aggcgagcca ttgttttcta aaggccggtc
     3421 tgacctctcc taatcttcaa ccaagcaggc gactcttcca aatgcgccga ctggcatcgt
     3481 tcatcacgat gcttttgaat acagctcaag cagtagccga tcacgggggc attaacagtc
     3541 tcctcatagt aacgtagcgg ccaattctcg caccctctgc agaaatgcca cgtatccgtc
     3601 aaattagatt ttcgataaaa agttctgata tcgaacatgc gaaatcctga tcagcatagc
     3661 tggttacgaa ccgcaggaga aaggggtggt gatgcaatcg atgactttgt cccaactccc
     3721 tgccaattca tcgcctatgt tggtgctctt catgccgccc cagaaaacca gagcgaccag
     3781 gaatatgatc agggtgtatt ccatcaatcc ctgaccccgc tcattacgca agttctttag
     3841 tggtctcatg actctcccct ttcgttgctt tatgcgatgc tgtggtatca caacccggct
     3901 tagcgcaact ctaatgccac ag
//
LOCUS       DASUVS010000205        14038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_83962, whole genome shotgun sequence.
ACCESSION   DASUVS010000205 DASUVS010000000
VERSION     DASUVS010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14038
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83962"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..237)
                     /locus_tag="VF182_11430"
     CDS             complement(52..237)
                     /locus_tag="VF182_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437734.1"
                     /translation="MAKLLRAAFVSAVKDPELKVEAEKRNYELDPVAGEDMEKLAKEV
                     MSQPQTVIDRMKKILGN"
     gene            527..661
                     /locus_tag="VF182_11435"
     CDS             527..661
                     /locus_tag="VF182_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437735.1"
                     /translation="MGIALAAQAQDLDPVFHKLKDGIYTFAPERVTTTCSFVITEAVF
                     "
     gene            817..1641
                     /locus_tag="VF182_11440"
     CDS             817..1641
                     /locus_tag="VF182_11440"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6437736.1"
                     /translation="MTLTATKVILLATLSMSYPPRSAVAQQEIVTLPTRTGVTQSYFL
                     TSIPQSLQALALLFPGSGGLIQLRAENGKPRFNQGNFLVRSRAEFIKRGVAAAIIDAP
                     SDQQSGWGMSDEFRLGNEHLSDISRIIDDLAKRFPEVPLFLIGTSRGTISAAALSRRL
                     GERTAGAVLTATMFRHTSRKSKEPGEGLSKFDFATVKVPLLLVHHVNDQCESTPYGDA
                     ARLSEKYRLISVFGGPTPQSGPCDAFSQHGFLGKESETVEQIVNWMMKRPFSQEVK"
     gene            1773..2753
                     /locus_tag="VF182_11445"
     CDS             1773..2753
                     /locus_tag="VF182_11445"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6437737.1"
                     /translation="MRYVQFGKSEFKVSQIGLGCMSMSGVYGPADDNESIATLHQAFD
                     LGINFIDTSASYGSGHNHELIAKALRGRRERIVIHSKSGSPRAPDASGMRSGSTPEYL
                     TQNCEQSVKRLGIDCLDIFCMSRVDPNIPVEESVGAMAQLVKRGLTRYVGLSEASATS
                     IRRARNVHPIVSLQMEYSLWSRDPEAGNIAACREFNMGFMAYSPLGRGFFAGAVHDVK
                     DMTDGDGRSNHPRFQPENLARNLKLLEQFEAFAQEKTATPSQLALAWLMAQGNDIIPI
                     PSNKSRRHLEENIKAVDLQLSKEDLARLDQIFPPGAAAGPRTKDLHRVNV"
     gene            complement(2855..3688)
                     /locus_tag="VF182_11450"
     CDS             complement(2855..3688)
                     /locus_tag="VF182_11450"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6437738.1"
                     /translation="MKLLITGGAGKVGQWLVRELLNNPTYELTVFDRTPGPERGIVKY
                     LVGDIQDLGQVIEAMAGAEAVIHLAAIHNPNIATTTVTYQTNVVGTFNVHHAAFRLGI
                     RRVISASSNAIVGWSYGERFMPEYLPIDEDHPLRPEDVYGLSKEIGETIARSYTRKGL
                     ETVMLRPSGVVTPDELEQMKKDQGRRASGFHAYSYVDARDLAVAFRLAIERTIPSGTI
                     LFIVADDSTVAEPLCELYPRIMPSIGDKARALTGSKGAYSNARAKQLLGWQPIHCWRC
                     E"
     gene            complement(3879..4445)
                     /locus_tag="VF182_11455"
     CDS             complement(3879..4445)
                     /locus_tag="VF182_11455"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6437739.1"
                     /translation="MFLRGSECDLLLSDSYFFPLYDAASRLDLPICIHSATGSPILFN
                     YFKYETIGFSKFKLVLVGAFHNLIMDGIPERFPRLKVAFLEVSAQWLPYVLTDLAKRY
                     HLHGRELNKKDLLRASRFYVGCETSDDLPYIIGHAGEDNLVVGTDYGHADSATELLAL
                     RGVLDDTRLSGSSANKIVSDNARALYGL"
     gene            complement(4464..4907)
                     /locus_tag="VF182_11460"
     CDS             complement(4464..4907)
                     /locus_tag="VF182_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437740.1"
                     /translation="MLKIDADAHVLETEKTWEYMEGPERKFRPQVVGSNDGSSDDEYW
                     LVDGTLRLKSRNVGKNTPMASRELRDVTARLKHIDELGVDIQVIFPTIFIIPLTPRPE
                     IDLALCRSYNRWMANIWRQAKDRLRWVAVLPLLSMDKVYDEAKFA"
     gene            5082..5933
                     /locus_tag="VF182_11465"
     CDS             5082..5933
                     /locus_tag="VF182_11465"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6437741.1"
                     /translation="MRRFDLVEPTSLEDACAFIANNDDAKAIAGGTALLTLIKHGIFV
                     PKTLVNLKKIQGASDISYGAQTGLRIGALTTIYDIETSPLVRQHYEVLAEACHVVANI
                     RIRNMATIGGNLAHGDYQSDPPTVLVALEAVVELLSRTGMRRMKLSDFLKGSYETAIE
                     PGELVSALIVPPADHLRGTYTKFTTGSSEERPCAGIAALASMDDSVCLELRLVVGAVS
                     PKPVRMQNGESMARGERLTQEMVERIAKDASRNVDPIDDLRGTADYKRHLVHVLTRRA
                     LSALAGV"
     gene            5947..8265
                     /locus_tag="VF182_11470"
     CDS             5947..8265
                     /locus_tag="VF182_11470"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6437742.1"
                     /translation="MPLVGTDVAMVGGLAKVTGAVNYSPDLVLPRMLYAKALRSPYPH
                     AKLLRVDASKAEKYSGVVAVVTHEDLTGLNPYFGPVVDDQPIVATDRVRHVGEVVALV
                     AAESREIAEEALDLIEVDYQELPAVFDLLEAAKPGAPVLHEQRNETNAGVHREEFNFE
                     LGGNVCSVFRVADGNIQKGFQEAEEIFENTYVLPPVQHGHIEPHAATAYWEPDGKLVV
                     HSASQNPSGVQEQLAHIFDLPENKVRVIVPILGGGYGGKTHARLEPALALLTRKARRP
                     VQWVLTRDEVFLTGRRYGAVVKIKTGVKRDGDIVARQVEVFYEIGAYALNGPVNAKTG
                     CYVSSGPYNIPNRSLSTYSVYTNLPPTGPFRGVGVSHVCWAYESEMDDIARRLGLDPL
                     ALRLKHLVKEGDVFVTGEKLVSVGITDCLERAAAAIGWKGKEEQSARPDGNNLIRGKG
                     LAVTIKSTTTPTTSSANVRLNADGSAILLTSSVDMGQGALTSLAQIVGDELGLPFEKV
                     SVSLPDTDITPYDKSTSSSRTTFHMGQAAQKAAREIRNQLLEVGAKKLEARIEDLELR
                     EGAVSVRGVPDKRLSITDIFKARFGSSVGSIFGGYCFKSEGGLNPKTGKGKAAAFWFF
                     SACGAEVEVDIETGKVRVLNIVTAVDVGKAVHPKQCGLQNEGSMLSGMGSALFEEMLY
                     DSGQPINSNFLEYMLPSMEDHPLEFHSVLVETPHPEGPFGAKGMGEAALPPVAPAIGN
                     AIANALGGIRIRDLPIKPDKIIAMRNARKERS"
     gene            8266..8727
                     /locus_tag="VF182_11475"
     CDS             8266..8727
                     /locus_tag="VF182_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6437743.1"
                     /translation="MTITISTTINGSRQEVAVRPNQTLLEFLRENLSLQGSVEGCGVG
                     VCGSCTVLVDGRPVSSCLMLASNAEGKKVTTIEGLSHSGELDPVQSAFLKHQAFQCGY
                     CTPGMIMAVKGLLANNPHPSEEQARDYLSGNLCRCGTYSEVLAAVQELAEE"
     gene            8833..9888
                     /locus_tag="VF182_11480"
     CDS             8833..9888
                     /locus_tag="VF182_11480"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437744.1"
                     /translation="MPTPVRIYAGTQEGLFVWRSKNSSWDRVAVSFETGTIDSIDGLR
                     SRPNVVYLGVTQDGLYRTQDAGKSWCRVFEGNVRAVTVDPTDEQVIYVGIEPIHLYRS
                     EDGGESWEELTGVQDLPAQVKGKWSYPRPPHREHVRHIFVHPDDPKILHVCLEHGGII
                     RSFDRGKTWQDVSGGIDYLDVHHISSAPSRKDLYFVATARGFFKSSDPGQGWQRAETG
                     FSRDYFHDFVFLPGATPAMLVATADKSPGYWNRPERAQGAVFRSRDFAESWERVGVGK
                     WLPENMQQMVWALAQHPEDPSIVYAGLGAVSRGRSADATQKGHGDILVSLDQGDSWDR
                     LPLDLPADRVLFVASDS"
     gene            9925..10968
                     /locus_tag="VF182_11485"
     CDS             9925..10968
                     /locus_tag="VF182_11485"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6437745.1"
                     /translation="MKSYRTACLGMTQSFLLVALLMILAEPTCAQTSDLIQSAKKEAE
                     VTVFGSLENDVAGAINKGFEAKYGIKTNYFRGSSTVIIDRITTEHRTGKMSSDVVYTT
                     AEPMKFINKEKGLLARYVSPSAKEYDKKLIDDFFGPNYRSVIIGFIYNKNMIKPEDAP
                     KSYEDIVSPKWKGKIAMGNPTLHDTTIDWLSSLHFVFGSTQKANEWIKKLAALEPLML
                     DSMVPVGERIASGEVPLGVAYVKYVHLWNRKGAPMDYVKGLPVYLGDGNYIGLTNKAP
                     HPNSARLFVDYFLGQESSEIMANVGEFVNRPGIYPPIEGAEQVAGRFVQVVSMTADQY
                     SKKKEEYRQIFRR"
     gene            complement(11362..12342)
                     /locus_tag="VF182_11490"
     CDS             complement(11362..12342)
                     /locus_tag="VF182_11490"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437746.1"
                     /translation="MKQLVSALLFVLFCAHSVFAADRMRIAYSSISGAYIGIWAAREA
                     GFFAQENLDDQIIFIPSATQLSQVIVAGDVDIASLGGGPIMAAAISGADMKIIGNNVN
                     KMIFSIHTKPEVKSIQELRGKRIGVTRFGSSSDVSARYALRKHNLDPQKDVILLQLGS
                     MTSIFGALKSGAIEAALVSPPTQFLSESLGFKELVSVTDMDYAYPNPAMAVPGALIRN
                     KPDSIDRFMRAYVRGVHRARTAREFAIKTLAKYTTVTDAVLLGKTYEFYIGKVVEPAP
                     YVNMTGLQNALDDLARSVPAAKTAKPEQFVDMRFLERVEKSGLLKELYKN"
     gene            12555..13538
                     /locus_tag="VF182_11495"
     CDS             12555..13538
                     /locus_tag="VF182_11495"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437747.1"
                     /translation="MKIHLSIAVALMSAIAIPANLDAQMKTRIAWTSFASNMAGTWVA
                     QEEGLFRKNGVEAELVHIPSTSRAIQTMLAGEIAFTYMDGRNSVQAGLKGIDVVILAG
                     VANRLVFSFMSRPEIKNFAELRGKKIGITRLGSSTHSVTLWTMSKMGLRPEEYQLLQL
                     VDVPNILTAIMAGQIEAGALSPPTNFRARKAGLNELIDLTKEGPEYVSVAIGSTRAFV
                     KANEDLTRRLIRGYSEGVQLLKANKAVGIRAIQKYARIKDPDILEATYGEARSYIETV
                     PYATKKGLETIISELASTEAKGRQAKPDDFLDTRFVSQLEKEGFYKPAAAR"
     gene            13649..>14038
                     /locus_tag="VF182_11500"
     CDS             13649..>14038
                     /locus_tag="VF182_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6437748.1"
                     /translation="MSNVKLPSGVDLYYESHGQGEPLVLVPSTAYSSDVWKPSQMPLA
                     HSLNLILFDPRGCGRSIPMQEVFTIEQMAVDIVALMDHLEIPSAHLLGHSMGGRIALS
                     MTQNFPGRVKSLIMAASGSGPAARLGTD"
BASE COUNT         3279 a         3664 c         3964 g         3131 t
ORIGIN      
        1 gaactagttg gtggtgcatt tgaccgccgc tttagaaact aaaaacttgt ttcagttccc
       61 cagtatcttt ttcattctgt caatgaccgt ttgaggttgc gacatcactt ccttcgcgag
      121 tttctccatg tcctcgccgg caacgggatc caattcataa ttcctttttt cagcttcgac
      181 tttgagttcg ggatctttga ccgcactcac aaatgcggcg cggagaagtt tcgccattca
      241 ccggtcgccg actatctcgg caacagcgtc ctcatggtaa tcttccaagt tacgcacgcg
      301 ccaaaggtta atgtcgatgt cgcacgtaat caaaggtcgc tgacaagggc tcatgataca
      361 aacgacactt atgggtcaat tgcatcgatg gcgttcgcga cgatttagca gatcatgtct
      421 tgacgcagta ctgcgattga actagcttcc cgtccagaaa ttcaaatcgc cgaagaggca
      481 gcaggatggc caaaatcaag catattattc ccgtgcagtc gttacgttgg gtatcgcatt
      541 agccgcgcag gcgcaggacc tcgacccagt atttcataag ctcaaggatg gcatctacac
      601 tttcgcgccg gagcgagtta cgacgacatg cagcttcgtg atcaccgagg cggtgttttg
      661 atgatcgatg cctagaacag cgcgttggat tcgcgccgaa aggcgtcagc gatcaggaga
      721 atgactcaca tacctgtcat tgatgtggaa tgagaaggtg gtcaagaact aacaagctaa
      781 agcggacttc tttcaatgcg aaccaaggcg atactcatga cattgacggc gacaaaagtg
      841 attctgctcg caacgttatc gatgtcctat cctccccgta gcgcggttgc ccagcaagaa
      901 attgtaactc tgccaacacg aacgggagta acccagtcgt atttcctaac gagcatcccg
      961 caaagtctcc aggcactcgc gctgctattt cccggttcag ggggcttaat acaactgcga
     1021 gcggaaaacg gaaagcccag attcaatcag ggtaattttc tcgtgcggag ccgcgctgaa
     1081 tttatcaagc gcggagttgc cgccgcgatc atagatgcac catctgacca gcagagtggc
     1141 tggggcatga gcgacgaatt ccgtctcggt aacgaacatc tgagtgacat ttcccgcatc
     1201 attgacgatc tcgcaaagcg atttcccgag gtgccgttat ttttgatagg tacgagccgt
     1261 ggaaccattt ccgcggcagc cctcagccgc cggctcggtg aacggactgc gggcgcggtt
     1321 ttgaccgcga cgatgtttcg ccatacttct agaaagtcga aagaaccagg cgagggtctg
     1381 agcaaatttg actttgcaac ggtcaaggtg ccattgctgt tggtacatca cgtaaacgat
     1441 caatgcgagt caacgcctta tggcgatgcg gcacggctgt cagaaaaata tcggctgatt
     1501 tcggtgttcg gcggacccac gccacagtct gggccatgcg atgcgtttag ccagcacggg
     1561 tttttgggca aggagtcgga gacggtggag caaattgtga actggatgat gaagcggcct
     1621 ttcagtcaag aagttaaata gaaggtcaac acccgatagt aaagcgctat aaagttcgcc
     1681 agattcaaga cttcaaagtt caatgcgttc agaaaccaaa attgctcgag aaggtctagc
     1741 gggagcatta ttgctcagag ggaggaagat tcatgcggta cgtacaattt ggcaagagtg
     1801 agttcaaggt ctcgcaaatc ggactgggct gcatgagtat gtcgggggta tacgggcctg
     1861 cggatgacaa tgaatccatc gctacgctgc accaagcttt cgatcttggc atcaatttca
     1921 tcgatacttc ggctagctac ggcagcggtc acaaccatga gctgattgcc aaggctctca
     1981 ggggcaggcg cgagcggata gtaattcatt caaaatcggg cagcccgcgc gcaccggacg
     2041 ccagcggcat gcgcagcggc agcacgccgg aatacctgac acaaaattgc gaacagagcg
     2101 tcaagcggct gggcatcgat tgcttggata ttttctgcat gtcccgggtc gatccaaata
     2161 ttccggttga agaatcggtg ggcgccatgg cgcaactcgt gaagcgcggg ttaacccggt
     2221 acgtcgggtt gtcggaagcg agcgcaacgt cgattagacg ggcgcgcaac gttcacccga
     2281 ttgtctcgct gcagatggaa tattcgttgt ggtcgcgtga tccggaagca ggaaatatcg
     2341 ccgcatgccg tgaattcaat atgggcttca tggcctactc accgctcgga cgcgggtttt
     2401 tcgctggtgc ggtccacgat gtcaaggaca tgacagatgg cgacggacgc agcaatcatc
     2461 cacgtttcca gccggagaat ctagcacgca acctgaaact gctggagcag ttcgaagctt
     2521 ttgcccaaga aaaaactgcg actccctcgc aactcgcctt ggcctggctg atggcgcaag
     2581 gcaacgacat cattccaatc ccaagcaaca aaagtcgccg tcacctcgaa gaaaacataa
     2641 aggcagtcga tctgcagtta agcaaggaag atctcgcgcg actggaccag atcttcccac
     2701 cgggtgccgc ggccgggccg cgcacgaaag atctgcatcg ggtgaatgtc tgagataggt
     2761 tcagatcgtt tcaatccgtg ctaatttttc ttggtccaag tcacgatccg ctcttccgct
     2821 attttgcacg aagagatgtt tctgtccggt cctttcattc gcagcgccaa caatgaatgg
     2881 gttgccagcc gagcaattgt ttggcgcggg cgttggagta tgctcccttg ctccccgtca
     2941 gggcacgggc tttgtcgccg atgctcggca tgatccgcgg ataaagttcg cagagcggtt
     3001 cggcgacagt gctatcgtcg gcgacgatga acaagatagt gccgcttgga atcgtgcgct
     3061 caattgccag tcgaaacgcg acagccagat cgcgcgcatc cacgtaactg tatgcgtgga
     3121 agccgctcgc tcgacggcct tggtcttttt tcatttgttc gagctcgtcc ggcgtcacca
     3181 caccgcttgg gcgcaacata acggtctcta accccttgcg ggtgtaagaa cgggcgatgg
     3241 tttcgccgat ctctttcgat agaccataga catcttcagg tcgtagcgga tgatcttcat
     3301 cgatgggaag gtactcaggc atgaagcgtt cgccatagct ccagccgacg atagcattgc
     3361 tgctcgcgga gatgactcgt ctgatgccga ggcggaaggc ggcatgatga acgttgaacg
     3421 tgccgacgac gttggtttga taggtcacgg tggtcgtcgc gatgttagga ttgtgaatcg
     3481 ccgccaggtg aatgacggct tcggcgccgg ccatagcctc gataacttgg ccaagatctt
     3541 ggatgtcgcc aactagatac ttgactatgc cgcgttccgg tccgggtgtg cggtcgaaca
     3601 cggtgagttc gtaagtggga ttattgagca gctcgcggac gagccattgg ccaactttgc
     3661 cggctccgcc ggtgatgaga agtttcatta tgtggattcg tctatcatgc ggattactta
     3721 tgacggcgca tgcatgtacc gattcgcatc ggagaattta ccgcggcggc gcagagggcg
     3781 aggaaaaagg tttttagaga aaactctgta tgcattctat gctctgcttt gaggttgtat
     3841 tttgatttat cacttcggga gcccgcattt ccaattgatc acaacccgta aagtgcccgg
     3901 gcgttgtcac tgactatctt attagcgctc gagccgctta accgggtatc gtccaacaca
     3961 ccacgcagcg ccaggagctc ggtggcgcta tcggcatggc cgtaatcagt accgaccacc
     4021 agattgtctt ctccggcatg accaatgatg tatggaagat catcgctggt ctcgcagccg
     4081 acatagaagc ggctcgcgcg cagcaagtct tttttattca attctctgcc gtggaggtgg
     4141 tagcgcttgg cgagatcggt tagcacgtag gggagccact gcgcgctgac ttcgagaaaa
     4201 gccaccttca gccttggaaa acgttcgggg attccgtcca tgatcaggtt gtgaaacgca
     4261 cctaccagta ccagcttgaa cttcgaaaag ccgatagttt cgtatttgaa gtagttgaat
     4321 aagatcggac ttccggtggc ggaatgaatg cagatgggca agtccaagcg gctggccgcg
     4381 tcgtagagcg gaaaaaaata agaatcgctc agcagcagat cgcattccga tccgcgcaga
     4441 aaaataccga ccgcgccgtt ctcctaggcg aactttgctt cgtcataaac tttgtccatg
     4501 ctgagaagcg gcaataccgc cacccaacgc agccgatctt ttgcctgccg ccaaatattc
     4561 gccatccagc ggttatagct gcggcatagc gcgagatcga tttccggccg aggcgtgagc
     4621 gggataatga agatcgtcgg aaaaatgact tgaatgtcga cgccgagctc gtcgatatgc
     4681 ttgagacggg cggtgacatc acgcaattcc cgcgaagcca tcggcgtgtt cttgccgaca
     4741 ttgcgcgatt tcagccgcag cgtaccgtcg accaaccaat actcgtcatc cgaggaacca
     4801 tcgttggaac cgacgacctg gggtctaaac ttgcgctcag ggccttccat atattcccaa
     4861 gttttttctg tttcaaggac gtgggcatcg gcatcgatct tgagcatgat caaacctctg
     4921 tcttaagttg tcttggttat ggtcattgtt agctgcttcg ttcataacac ccgttggagc
     4981 tgggcgtcaa cgaagatgct tgacacagct tgactctgtg atagtagcgt tttcaacgac
     5041 gcgacgatca ttttccgctg cgagatcagc aaagccaagc aatgcgacga ttcgatttag
     5101 tagaaccaac cagcctcgag gatgcttgcg ctttcatcgc caacaatgac gatgccaaag
     5161 ctatcgccgg tggtacagcg cttttgactc tgatcaaaca tggcatcttc gttccaaaaa
     5221 cccttgttaa tttgaaaaaa attcaaggcg ccagcgacat cagctatggc gcacaaaccg
     5281 ggctgcgtat cggcgcactg acgacaatct atgatatcga aacctcgccc ctagtacgac
     5341 aacactatga ggtgctcgct gaagcgtgcc atgtagttgc gaacattcgc attcgcaaca
     5401 tggctaccat aggcggcaat ctggcgcacg gcgactatca atccgatccg cccacggttc
     5461 tggtggcgct ggaggccgtc gtcgaacttt taagtcgcac aggaatgcgc cggatgaaat
     5521 tatccgactt cttgaaaggc agttacgaga ccgcgatcga gccgggagaa ttggtgtcgg
     5581 ccttgatcgt tccgccggcc gatcatcttc gcggcaccta cactaaattt accaccggct
     5641 catcggaaga gcggccctgc gccggaattg ccgcattagc gagcatggac gacagcgttt
     5701 gcttggagct tcgtttggta gtcggcgcgg tatcgcctaa accggtgcgg atgcaaaacg
     5761 gcgagtctat ggcgcgcgga gagcggctta cacaggagat ggtcgagcgc atcgccaaag
     5821 atgccagccg taacgttgat ccgatcgatg acttgcgtgg caccgccgat tacaagcgcc
     5881 acctggttca tgtgctaact cgccgggcgc taagcgctct ggcgggagtc taagaaggaa
     5941 cgaagaatgc ctctggtcgg aacagatgta gcaatggtag gagggctggc caaggtcact
     6001 ggagcggtga attactcacc tgatctcgta ctgccgcgca tgctctatgc caaggcgctg
     6061 cgcagcccgt atccgcacgc aaagcttttg cgggtggacg cgagcaaggc ggaaaaatat
     6121 tcgggcgtcg ttgctgtcgt cactcacgag gatttaaccg gcttaaatcc ctattttgga
     6181 ccggtggtcg acgatcaacc catagtcgct accgatcgcg tgcgccatgt cggtgaagtc
     6241 gtcgcgctcg tcgccgcgga aagccgcgag attgccgaag aggcgctgga tttgatcgaa
     6301 gtggactacc aagagttgcc ggcagtattc gatcttctcg aagccgccaa gcctggcgcg
     6361 cccgtactgc acgagcagcg caatgagacc aatgcaggcg tgcaccgcga ggaattcaat
     6421 ttcgagttgg gcggcaacgt gtgcagcgtg ttccgcgtcg ccgacggcaa catccaaaaa
     6481 ggttttcaag aagccgaaga gatttttgag aatacctatg tgctgccgcc ggtgcagcac
     6541 ggtcatatag agccgcatgc agccactgcc tattgggagc cggacggcaa attggtggtt
     6601 cattccgcct cgcaaaatcc ttccggcgtg caggagcagc tggcacatat ttttgatttg
     6661 ccggaaaata aggtgcgcgt gatcgttccc attctcggtg gcggctacgg cggcaagacc
     6721 catgcaaggc tggagcccgc tctggcgcta ctcacacgca aggcgcgccg cccggtgcag
     6781 tgggttttga ccagggacga ggtctttctc accggcagac gctacggcgc ggtggtgaaa
     6841 atcaaaaccg gagtaaaacg cgacggtgac atcgtcgcgc gtcaagtcga ggtcttttat
     6901 gaaatcggcg cttatgcgct caacgggccg gtcaacgcca aaaccggctg ttatgtctca
     6961 agcggtccct acaatattcc caaccggagt ctttcgacgt actctgttta cacgaatttg
     7021 ccacccaccg gaccgtttcg cggtgtcggc gtttctcatg tctgctgggc ttacgaatcc
     7081 gagatggacg atatcgcgcg gcggcttggc ctcgatccgc tggcgttgcg cctgaagcac
     7141 ctcgttaaag agggtgacgt attcgtcacc ggggaaaagc tggtttctgt cggcatcacc
     7201 gattgtttgg agcgcgccgc tgcagcgatc ggctggaaag ggaaggaaga acaatcagca
     7261 cggccggatg gaaacaacct aatccgcggc aaagggcttg cggttacgat caaaagcacg
     7321 accacgccga cgacgtcgtc ggcaaatgtg cgtctgaacg ccgacggttc agctattctt
     7381 cttaccagca gcgtcgatat ggggcagggg gcattgactt cgctggcgca aatcgtcggc
     7441 gacgagttgg ggttgccgtt tgagaaagtc tccgtgagct tgcccgatac cgacattaca
     7501 ccctacgata agtcgacgag ctcaagccgc acgacctttc atatgggaca ggcggcgcag
     7561 aaagcggcgc gcgagatcag aaatcagttg ctcgaagtcg gggcgaaaaa actcgaggcg
     7621 cgaatcgagg atctagaatt acgggaaggc gccgtgtcgg tgcgaggcgt tcccgataaa
     7681 cgcctgtcga tcacggacat cttcaaggcg cgttttggat cgtcggtcgg cagtatcttc
     7741 ggcggctact gctttaagag cgaagggggt ttgaacccca agaccggaaa gggaaaggct
     7801 gcggcattct ggttcttctc cgcctgcggc gctgaagtcg aagtcgatat cgaaacaggc
     7861 aaggtacgtg tcctgaatat cgtgacggcg gtggacgtgg gcaaagcagt tcatcccaaa
     7921 caatgcgggt tgcagaatga agggtcgatg ttgtcgggaa tgggctcggc gctctttgaa
     7981 gaaatgcttt atgacagcgg ccagccgatc aacagtaact tcttagagta catgctgccg
     8041 tcaatggaag atcatccgct ggagtttcat tccgtgttgg tcgaaacgcc ccaccccgag
     8101 ggccccttcg gtgccaaggg aatgggagaa gcagcgttgc cgccggtagc accggccatc
     8161 ggcaatgcca tcgccaatgc gctgggcggc attcggataa gagacttacc gatcaagccg
     8221 gataagatta tcgcgatgcg taacgcgcgc aaggagcgaa gctaagtgac gatcaccatc
     8281 tcgaccacga tcaatggatc gcggcaagag gtcgccgtta gaccgaatca gacgctgttg
     8341 gagttcctac gcgagaatct ctcgcttcag ggttccgtgg aaggctgtgg cgtgggagtc
     8401 tgtggttcct gcaccgtact ggtcgatggt cgtccggtga gctcctgtct catgctcgcc
     8461 agcaatgccg agggcaagaa ggtaactacc atcgaaggtt tgtcgcacag cggcgaactc
     8521 gacccggtgc agagcgcatt tctcaaacat caagcttttc agtgcggata ctgcacgccg
     8581 ggcatgatca tggcagtgaa gggactgctc gcgaataacc ctcatcccag cgaagaacag
     8641 gcacgcgatt atctgtcggg gaatctttgc cggtgcggca cctatagcga agtgctggcc
     8701 gcggtccaag aattggcgga agagtgagag cgctggagtt agggcggact gggtagtgga
     8761 gtgtgggagg ccccgaatac tcgataaccg ataagtggcc ccagcgaatt ggggcgggaa
     8821 aggagttttg tcatgccaac tccagttcga atttatgccg gtacccaaga gggattgttc
     8881 gtctggcgtt ccaagaatag ttcctgggat cgtgtcgccg tttctttcga gaccggcacc
     8941 atcgattcca tagacgggct ccgcagtcga cccaacgtcg tctatctcgg cgtcactcaa
     9001 gacggtctct atcgcaccca agatgccggt aagagttggt gccgggtgtt cgaaggcaac
     9061 gtccgcgccg tcaccgtcga tcccacggat gagcaagtga tctatgtggg tatcgaaccg
     9121 attcaccttt accgcagcga ggatggcggc gagagctggg aggaattgac gggcgttcag
     9181 gatctgcctg cacaagtcaa aggcaaatgg agctatccgc ggccgccgca cagagaacat
     9241 gtacgccata tcttcgtcca ccccgatgat cctaaaattc tccacgtctg cttggaacat
     9301 ggcgggatca tccgcagctt tgaccgaggc aagacctggc aggatgtcag cgggggcatt
     9361 gactatttgg atgtccatca catcagcagt gcgcccagcc gcaaagatct ttatttcgtc
     9421 gctaccgctc gaggattttt taaaagtagc gatccgggcc agggttggca gcgcgccgag
     9481 acgggcttct cgcgcgacta cttccacgat tttgtttttt tgcccggcgc cacgccggcg
     9541 atgctggtgg ccacggcaga caagtcgcct ggttattgga atcgcccgga gcgcgctcag
     9601 ggcgcggttt ttcggagccg cgattttgcc gagagttggg agcgtgtcgg ggtgggaaag
     9661 tggctgccgg aaaacatgca gcagatggtc tgggcattgg cgcaacatcc ggaagatccg
     9721 tctatcgtct atgccggtct cggcgcggtc tcgcgcggcc gctcggcaga cgccacccaa
     9781 aaaggacatg gagacatact ggtcagcctg gatcagggcg acagctggga ccggttgcca
     9841 ttggatttgc cggccgatcg cgtgctcttt gtggcttctg attcttgatt ttcgaagcgc
     9901 tgaccaagcg caacggagga gatcatgaaa tcttatcgaa cagcctgcct aggcatgact
     9961 caatcatttt tgctcgttgc gttgttgatg atactcgccg aaccaacctg cgcgcaaact
    10021 tcagatctca tccagagcgc caagaaagaa gccgaggtca ccgttttcgg atccctcgaa
    10081 aatgacgtcg ccggagccat caacaaggga ttcgaggcga agtatggcat caagaccaac
    10141 tacttccgcg gctcgtcgac ggtgatcatc gatcgaatca ctaccgagca ccgcacgggt
    10201 aaaatgagct cggatgtcgt ttacaccaca gcggagccca tgaagttcat aaacaaagag
    10261 aaaggactcc tggctcgtta cgtgtcacca tcggcgaaag agtatgacaa gaaattgatt
    10321 gacgacttct ttggccccaa ctatcgcagc gtgattatcg gcttcatcta caacaaaaac
    10381 atgattaagc cggaggatgc gccaaaatct tacgaggata tcgtcagccc aaagtggaaa
    10441 gggaagattg ccatggggaa tccgactctc cacgacacca ctatcgactg gctctcaagt
    10501 ctccatttcg tcttcggctc gactcagaaa gccaatgagt ggataaaaaa gctcgccgct
    10561 ttggagccgc ttatgctgga ttcaatggtg ccggtcggcg agcgcatcgc ctcgggagaa
    10621 gtgcccttgg gtgttgctta cgtgaagtat gttcatctgt ggaatcgaaa aggcgcaccg
    10681 atggattacg tgaaaggact tcctgtatat ttgggcgacg gcaattacat cggtcttacc
    10741 aacaaagcgc cgcatcccaa ttcggcgagg cttttcgtcg actatttcct cggccaggag
    10801 agctcggaga taatggccaa cgtgggtgag ttcgtcaacc gcccgggcat ttatccgccc
    10861 atcgagggcg cggagcaagt cgccggtagg tttgtccaag tggtgtccat gacggcggat
    10921 caatactcaa aaaagaaaga agagtatcgc cagatctttc ggcgatagtc gaaacacaat
    10981 ccggactttc acagcactgc gccatgacac ggcaacacaa gcctgcaaag gcgttttttc
    11041 ggaaccatcg ctcagctcag agcagtggct gtcgagcgag tctttctggt tcatggcgca
    11101 aggcgacgac ctcattctaa tcaggcaaca agacgcgccg tcatttggaa aaaaatatca
    11161 gggcgatcga tatttggttg agcaagggcg atctcgcgcg tcgaccagat tttcccgcca
    11221 agagccgcgg ccgcggcccc gcacgaagga tttgcatcgg gtaatgtaga gcgtcgaact
    11281 gttttaaatc gtttcattcg ctcaagcagc cgaccgcgtg cgctcggttg agcttttgcg
    11341 gcaaaacgtc tcgacgctgt tctagttctt gtagagctct ttgagcagcc cgctcttttc
    11401 aaccctctca agaaaacgca tgtcaacaaa ctgttccggc ttggcggttt tcgcagccgg
    11461 caccgaacgc gccagatcgt ccagagcgtt ttgcagacct gtcatattga cgtaaggcgc
    11521 cggctcgacc actttgccga tgtaaaattc ataagttttc ccgagcaaca ccgcgtccgt
    11581 gacggtggtg tacttagcca gcgttttgat agcaaactca cgagcggttc tcgcgcggtg
    11641 tacacctctt acgtaagcgc gcatgaagcg gtctatggaa tcaggtttgt tgcggatcag
    11701 cgcccctggc actgccatcg ccggattcgg atacgcgtag tccatatccg taacgcttac
    11761 cagttcttta aaacctaagc tctcggacag aaactgtgtc ggcggcgaaa ccagagcggc
    11821 ttcgatagct cctgacttca aagcgccgaa gatcgaggtc atggaaccca gctgaagaag
    11881 aattacgtct ttttgcggat cgaggttgtg ttttcgcaac gcgtaacgcg cagaaacgtc
    11941 ggagctcgaa ccgaatcgcg tcacaccaat gcgtttgccg cgtagttctt ggatactctt
    12001 cacttcgggc ttggtatgaa ttgaaaagat catcttgttc acgttgttac cgattatctt
    12061 catatcggca ccgctgattg cggccgccat gatcgggcca ccgcccaagg acgcgatgtc
    12121 cacgtcgccg gcaacgatca cctgcgacag ctgcgtcgcg ctgggaataa agataatctg
    12181 atcatccaaa ttttcctggg cgaagaaacc ggcttcacga gcggcccaga tgccgatgta
    12241 ggcgccgctg attgaactgt aggcaatgcg cattctgtcg gcggcaaata cgctatgcgc
    12301 acagaagagt acgaatagaa gagcgctgac gagctgtttc atactgagct ccttttagaa
    12361 tggtatccat taggactaca ctcgtcgttt ccgagatttg cttttccgat catacaacga
    12421 tcagtaaaca ctgcgccact cgccagagcg cgtttgcagc ctagagctcg atcgttgccg
    12481 cttggtctcg acggtcgaga aggttcatga tattgagctg ccattctcat cgaataagcc
    12541 ccagggaacc gaacatgaag atccacttga gtatcgcagt ggcattgatg agcgcaatag
    12601 cgattcctgc aaatttggac gcgcaaatga agacgcgcat tgcctggact tcgtttgcga
    12661 gcaatatggc tggaacttgg gtagctcagg aagaaggact gtttagaaag aacggagtcg
    12721 aagccgaact ggtgcatatt ccttcgacgt cccgggcaat ccagaccatg ctcgccggcg
    12781 agattgcgtt tacctacatg gatggccgta attcggtaca ggcgggattg aagggaatcg
    12841 acgtcgtcat tcttgctggc gtcgctaatc ggctggtatt ttcctttatg tcgcggccgg
    12901 agattaaaaa ctttgccgaa ctcagaggga aaaagatcgg tatcactcgc ttgggatcct
    12961 cgacacacag cgtaaccctc tggacaatgt caaaaatggg cctcaggccg gaagagtatc
    13021 agctacttca gctggtcgac gtgcccaata tcctgaccgc gattatggcc ggtcagatcg
    13081 aggccggcgc tttatcacca ccaacgaatt ttcgcgcgcg taaagccggg ctcaacgaac
    13141 tgattgatct taccaaggag ggacccgaat acgtctcggt cgcgatcggt tccacccgtg
    13201 ctttcgtaaa ggcgaatgag gacctaacgc ggcgtctgat ccggggttat agcgaaggag
    13261 tccagttgct caaagccaac aaggccgtcg gaataagagc gattcaaaag tatgcgcgga
    13321 tcaaagatcc ggatattctg gaagcgactt atggcgaagc gcggagctac atcgagacag
    13381 ttccctatgc gacgaaaaaa ggcctcgaaa ccatcatcag tgaattggcc tcgacggaag
    13441 caaagggaag acaagcgaag cccgatgatt ttctagacac gcggttcgtg agtcagttgg
    13501 agaaggaagg tttctacaaa ccagcggccg caagatagcg gtatctcgga agctcattgc
    13561 ttcgagaaaa catcgacgtc aatgaatcaa ccatcgagat ctgggactca tgttcttata
    13621 gcttggttga ttggaaggga tcatttttat gtcgaacgta aaactgccga gcggtgttga
    13681 tctttactac gaatcccacg gtcagggcga gccgttggtt ttggtgccat caacggctta
    13741 ttccagtgat gtttggaaac cgtcacaaat gccgctggcg cattctttga atttgattct
    13801 ctttgatcca cgcggttgtg gtcgatccat tccgatgcaa gaggttttta caatcgagca
    13861 aatggccgtg gacatagtag ccttaatgga tcatctggaa attcccagcg cgcacctttt
    13921 gggtcattcc atgggcggca ggatcgcctt atcaatgact cagaactttc cgggtcgggt
    13981 gaaaagcctg atcatggctg cgagcggttc aggtccggcg gcgcgtctgg gaacggac
//
LOCUS       DASUVS010000206         8740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_957626, whole genome shotgun sequence.
ACCESSION   DASUVS010000206 DASUVS010000000
VERSION     DASUVS010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957626"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /gene="secD"
                     /locus_tag="VF182_11505"
     CDS             <1..648
                     /gene="secD"
                     /locus_tag="VF182_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX6437749.1"
                     /translation="RIGGGRASITGSFDIKEARDLAIILRAGALPAPVEIAEERTVGP
                     SLGQDSIRQGINSFIVGGALVIVFMIAYYKGAGLLAVVALLFNLLFLIAILAGFQAVL
                     TLPGIAGIVLTIGMAVDANVLINERIREELRAGRAPRSAVEAGYQHALPAILDSNVTT
                     FLSGVILFQFGTGPIKGFAVTLCVGILTTVITAVYLTRIYYDYRASSRKLARVSV"
     gene            655..1557
                     /gene="secF"
                     /locus_tag="VF182_11510"
     CDS             655..1557
                     /gene="secF"
                     /locus_tag="VF182_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX6437750.1"
                     /translation="MELIKPGLKIPFTRYRKIAVIVSTIVNLAVLIMLFTKGPNLGVD
                     FAGGTMVQLKFQQSVTIPEVRQAFENMNLGGSVIQDFGQPGANEYLVRLDQTSVEIGM
                     LGEQIKKVLTDQFGADRFEVRRIESVGPKIGEDLRQRGALSVIVATIMMGIYIWFRFD
                     LRFGIGAVIALVHDVLVAVGALMLANYEFDLTIIAALLTIVGFSVNDTVVICDRIREN
                     MRKIKRENLEGIINISINETLSRTILTTGTALLVLISLYALGGAVIRPFAFTLLVGFI
                     SGVYSTIFIASPVILMWEKTPVKK"
     gene            1667..3409
                     /gene="recJ"
                     /locus_tag="VF182_11515"
     CDS             1667..3409
                     /gene="recJ"
                     /locus_tag="VF182_11515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX6437751.1"
                     /translation="MQEELRKRWILKQPDQGFVSRCCDELKLSALLARMIALRDLTAI
                     DTARCYLSSSLRSDLPSPFLMADMDAAVNRIVHAIQASESIGIWGDYDVDGTTGASVL
                     VSFLREIGVEAIFYVPHRIEEGYGLNIEGLRRLKERGVGLVVTVDSGISNFKEVAAAR
                     EIGIDIVIVDHHQPPEQLPPAIAVVNPHRIDCAFPDKGLCAAGLAFYVVIALRARLRD
                     AGWFRASGDPDLRRHLDIVALGTIADMVPLKGVNRTLVKRGLTALGCSTRPGVVALKQ
                     IAGFTPGDVTAGQVGFQLGPRINAAGRMDAAIKVIELLTTESAEAALRIARELDDNNR
                     ERRAMEAEVLKTALQQAEEVIRCRHYSLVLSGEGWHPGVLGIVASRVVERFHRPTIVL
                     GFHAGEGKGSARSIRGFHMVQGLRRCSHLLEKFGGHEYAGGLTIKEENLPSFIEVFDE
                     AARAVLSPEALLPFLEIDAQLSFTEIGHALMREIEVLKPFGVGNPEPIFLSQGIEVCE
                     RKDFSMGARFRFRQAGRFVSGVAFGVKENFAARPGAIVDLVYRLSENEWNGNSIVEVK
                     VVDARPTGSSIAQV"
     gene            3486..4499
                     /locus_tag="VF182_11520"
     CDS             3486..4499
                     /locus_tag="VF182_11520"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437752.1"
                     /translation="MRRKRRRCLLALALFLTVMAGPSFGADIGGKPEKADVVVTYAQP
                     SGAFTPIWVAYEAGLFKKYGLNASLQLLTPQVSAQAVISEEADFYTDGPDLINARLRG
                     GQVKYIGGTMQQLVFQIWGAKEIKSIKDLKGKTIAASTPRAALDTATREALKKNGLVP
                     DKDVQILYVQSVPAILSSVLGGKTSAGTLSAPNTLKAKESGLNLLVDIGKLNIPAFQV
                     AYGTTEKYLKNNPNTVYAFLKAMAEGVVMSKKDRDVAKKAIGKYGKIEDPATIDGTYE
                     AFAPYWALSLAIRPEAIRAQFEYLDPKEFPNAKNADPKDFFDNSFVDSLAKSGFLKQL
                     GMK"
     gene            4546..5274
                     /locus_tag="VF182_11525"
     CDS             4546..5274
                     /locus_tag="VF182_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEX6437753.1"
                     /translation="MNRAGLDATSRRDIVYLALAAFFVTNAILGELTGGKLFTLGPFT
                     MSIGVIPWPVVFITTDLINEYFGRDGVRRLTLTTIGLIAYAFVVLYLSIQVPAASFSP
                     VNDTQFQAVFGQSLWIIVGSIVAFGISQMVDVGVFWLVRHRTGGKHLWLRATGSTLVS
                     QLIDSIVIIGIAFWLPGKVKTEEFFIVAGTNYSYKMMIAVGVTPLLYLGHSIIDRYLG
                     IEESHRLIESSAQADAELAVAEPN"
     gene            complement(5267..7105)
                     /locus_tag="VF182_11530"
     CDS             complement(5267..7105)
                     /locus_tag="VF182_11530"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6437754.1"
                     /translation="MQHTKDTRQLTWLLAWAVVFCDIGTSVYYVPGILYGNVGDNIPF
                     FVLLTTIGFIPLALKYIEISWRNPEGGGVVSITTKALGPMWGCLGGMLITTSYFLTAA
                     ISAVSGFHYIGSIVHLIDANIVLFACIGLLALALLNIVGIRESATVALVMAIAALSVD
                     LIVIAVSFWQFSPAQLWHAVQSFAPRQNLSFRELLVGFGAAWLAFSGLESISQLSPNM
                     RFPLRRTAKWAMIGVVITTVITSPLLTMLSVNLLPAEIKATQSERFISEVALVSGGLG
                     LNIAVVLSASTLLLFAANTAIIGAYHIFLALTNLGFLPRAIALRSATFRSPHIAIAIA
                     TIVPVAVILATEGELALLGEMYAFGLLGAFLFSSLSLDVIRWRLGRRDFAFWVGVLTT
                     GVILVAWCINLVEKELATLFGGVLTAIGMSIAVGVRRAWFLDLLHKVPWIQRLQARAY
                     MASEALVDEELRGLVTLGDAVELKNLYPSSTLLAVRGENPRLVQEGIRRAKGMGESAL
                     YCVYVEEWPGLFAGDTPHRPSDEGVITLRSALQEIHGKGLEAIPIWSVSYNAPEAIAN
                     AAKALDVDAVLIGASQRSAFYHMLRGHVLKGLMKKLPHDCHLMIVN"
     gene            7216..8373
                     /locus_tag="VF182_11535"
     CDS             7216..8373
                     /locus_tag="VF182_11535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6437755.1"
                     /translation="MPLPVQYFQKLVESCPDIIISVDKYGVITFYNDGARQNLGFSHE
                     EVLGKSVLAIYPTLDEARRVMTALRAGSGEGQGRIRSFETTFKTKKGELIPVAISASI
                     IYDDNGQEIGSIGFAKDLRDIRRRDQLVTLAEIAIGVSHEVNNSLEVLVNQVELLQKY
                     VERVASDEDYIVESDRLASVASQVKKIQDITARISQMADEGEYGTKEYLSGKMMTDLR
                     PGLNGKGCERPKPDERYPLRGLKILIVDDDAGICQSLRDLLEAEDCEVQTAPSGVYAM
                     QWLERSQFDLVLSDVVMPDIDGYQLYQRVKKDAPELPVILMTAFNYDKDHIIKRSCLE
                     GLQGVIFKKPVNPMLLKKALLQQCRPERVHQQAAAGSSPQTTPPPDSKRLR"
     gene            8375..8722
                     /locus_tag="VF182_11540"
     CDS             8375..8722
                     /locus_tag="VF182_11540"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6437756.1"
                     /translation="MSKEDFYNKGMELFMEENFDAAIAAFQQALAEDAKYTDALHALA
                     MTYSHQEKLDDAIETGKKLIEVAPDDELAHTSLSIFYQQKGMIAEAEHIAAQARTLGW
                     KRQLSQKNEPQKS"
BASE COUNT         2082 a         2226 c         2439 g         1993 t
ORIGIN      
        1 cgaatcggcg gcggcagggc ttccatcacc ggcagcttcg atatcaagga agcgcgtgat
       61 ttggcaatta ttctgcgcgc cggcgcgctt ccggcgccgg tggagattgc cgaggagcga
      121 acggtgggcc catccctggg gcaagactct attcggcagg gcatcaattc tttcattgtc
      181 ggcggcgcat tggtgattgt ttttatgatt gcctactaca aaggagcggg cttgctggcg
      241 gtggtggcgc tgcttttcaa tctcctgttt ctgatcgcga ttctagctgg tttccaagcg
      301 gttctgacct tgcccggcat tgccggtatc gttcttacca tcggcatggc cgtggatgcc
      361 aacgtattga tcaatgagcg gatccgcgag gagcttcgcg ccgggcgcgc gccgcgatcg
      421 gctgtcgagg caggctacca gcacgcgctg ccggcgattt tagattccaa tgttacgaca
      481 tttctttccg gagtgatctt atttcaattc ggcaccgggc caatcaaagg ctttgcggtg
      541 actctatgtg tggggatctt gacgaccgtc atcaccgctg tctacctgac ccgcatttat
      601 tacgattatc gagcgagctc tcgcaagctc gcccgtgtca gcgtctgagg aagtatggag
      661 ctcatcaaac ctggcttaaa gattccattt actcgctacc gtaaaatcgc cgttatcgtt
      721 tccacgatcg tgaatcttgc ggttctgatc atgctgttta ccaagggtcc caatttaggg
      781 gtcgactttg ccggcggtac aatggtgcag ctcaaatttc agcaaagcgt gacaataccc
      841 gaagtccgtc aggcgttcga gaacatgaat cttggtggaa gtgtcataca ggacttcggt
      901 caaccgggcg cgaacgaata tcttgttcgc ttggaccaga cctcggtgga gataggcatg
      961 ctcggtgagc agataaaaaa agttttgacc gatcagttcg gcgcggaccg cttcgaggtg
     1021 cgtcgaatcg aatccgtggg cccgaaaatc ggcgaggatc tgcgccaacg cggcgcgttg
     1081 tcggttatcg tcgccacgat tatgatgggc atctacatct ggtttcgctt cgatcttcgt
     1141 ttcggtatcg gcgctgtcat cgctctggtg cacgacgttc tcgtggctgt gggcgctctc
     1201 atgctggcca attacgaatt tgacctgacc atcatcgcgg cgttgctgac gattgtcggg
     1261 ttttcggtta acgatacggt cgtcatctgc gaccggatcc gggaaaacat gcgcaagatc
     1321 aagcgagaaa acctcgaagg catcatcaac atcagcatca atgaaactct tagccgaact
     1381 attcttacca ctggcaccgc tcttctcgtg cttatttctc tttatgcgtt aggcggtgcg
     1441 gtgatacggc cgttcgcttt tactctcttg gtgggcttta tctccggggt ttactcgacg
     1501 atcttcattg ccagtccggt gatcctgatg tgggagaaaa cgccagtgaa gaagtaatcc
     1561 atgtatgaga catccgagac gaaaactcgc aatgcttcaa ccggcgatta aaacctgagg
     1621 ccggtcgccg tatccactct ctctgatcat tcatttcacg acaacgatgc aagaggaact
     1681 ccgcaaaagg tggattctta aacagcctga tcaaggcttc gtcagccgat gctgtgacga
     1741 gcttaagcta tcggcgcttt tggcgcgcat gattgcgctg cgcgatctca cggcgattga
     1801 caccgctcga tgctatcttt cttccagcct ccgctccgat ctgccatcgc cgttcttgat
     1861 ggcggacatg gatgctgcgg taaaccggat tgttcacgcc attcaggcat cggaatcaat
     1921 cggcatttgg ggtgactacg acgttgacgg caccaccggc gcctcggttc tcgtctcatt
     1981 cctgcgtgag atcggtgtgg aagcgatttt ttatgttcct caccggatcg aggaaggcta
     2041 cggcctcaat atcgagggcc tacggcgact caaagaacgc ggagtcggac tcgtggtgac
     2101 ggtcgattcg ggaatttcaa atttcaaaga agttgctgcc gcgcgtgaaa tcggcatcga
     2161 tatcgttatc gtcgaccatc atcaaccgcc tgaacaactt cctccggcga tcgcggtggt
     2221 caatccacac cgaatcgatt gcgcgtttcc cgacaaagga ctttgcgccg caggtttagc
     2281 tttctatgtg gtgattgcgc tgcgagcccg gctccgtgat gcaggctggt ttcgcgcttc
     2341 cggtgatccc gatcttcgtc gtcatctcga tatcgtggca ttggggacga ttgccgatat
     2401 ggtgccgctc aagggcgtta accgtactct agtaaagcgc ggtttaacag cgctcgggtg
     2461 ttcaacgcgc ccgggggtgg tggcattgaa gcaaatcgcc ggattcactc cgggcgatgt
     2521 cactgctgga caggtagggt ttcaattagg accgcggatt aacgccgccg gacggatgga
     2581 tgctgccatc aaagtaattg aactgcttac gacggaatcg gctgaagcgg cgctgcgaat
     2641 cgctcgagag cttgacgata ataatcgcga gcggcgtgcc atggaggcgg aggtgctcaa
     2701 aacagcgctc caacaggccg aggaagtcat ccgatgcagg cactattcct tagtattgag
     2761 cggtgaaggc tggcatcctg gtgtgctcgg cattgtcgcc tcccgggtgg tggagagatt
     2821 tcatcggcca acgatagtgc tggggtttca tgcgggagaa gggaaaggct cggcgcgtag
     2881 catccgcggc tttcatatgg tgcagggact tcgtcgctgc agtcacctct tggaaaaatt
     2941 cggcggccat gaatatgccg gcggcctaac gatcaaagag gagaatcttc ccagcttcat
     3001 cgaggttttt gatgaagcag cgcgcgctgt gctgtcgccc gaggctctac tgccgttcct
     3061 ggagatcgat gcacagctca gtttcacgga aatcggtcat gcgctgatgc gcgagattga
     3121 agttttgaaa ccttttggtg tgggcaatcc tgaaccgatc tttttaagcc aaggaatcga
     3181 agtctgtgaa cgcaaggatt tctcgatggg ggcgcgattc cgctttagac aggcaggacg
     3241 gttcgtcagc ggcgtggcat ttggcgtgaa agaaaatttt gccgcgcgtc ctggtgcgat
     3301 cgtcgatcta gtttaccggc tgagtgaaaa tgaatggaat ggaaactcaa tcgttgaggt
     3361 caaagtcgtc gatgcgaggc cgacgggcag tagcattgct caagtatgac tgtccggtaa
     3421 gcgttgggcg tgcgcacgga gcaataaaga ggggagagga taataaaatg gacataagat
     3481 gcggcatgcg aagaaaacgt cggagatgtt tactggcact tgctttgttt ttaaccgtga
     3541 tggcgggccc gtcgtttggc gcagatatcg gaggtaagcc ggagaaagcg gatgtggtcg
     3601 tcacgtatgc ccaacccagc ggcgctttta cgccgatttg ggttgcctac gaagcggggc
     3661 tgttcaagaa atacggattg aatgccagcc tgcaactcct gacgccccaa gtgtcggcgc
     3721 aggcggtgat atcagaagag gcggactttt acaccgatgg cccggacctg atcaatgccc
     3781 gattgcgggg tggtcaggtg aaatatatcg gcggcactat gcagcagttg gtgtttcaga
     3841 tctggggagc gaaggaaatc aagtcaatca aagatctcaa aggaaaaacg attgcggcaa
     3901 gcacgccgag ggcagcgttg gataccgcta cccgtgaagc cttgaagaag aatggcttgg
     3961 ttccagataa agatgtgcaa atactttacg tccaaagcgt gccggcgatt cttagctcgg
     4021 tgctcggtgg caaaacttct gccgggactt tgtcagcgcc caatacgctc aaagccaaag
     4081 aatccgggct caaccttttg gtcgatatag gaaagctcaa cattccggcg ttccaagtgg
     4141 cgtatggcac gaccgagaag tatttaaaaa acaaccccaa cacggtttat gcatttctaa
     4201 aagccatggc cgaaggagtt gtcatgtcaa aaaaagatcg cgacgtggcc aaaaaggcga
     4261 ttggcaaata cggaaaaatc gaggatccgg cgacaatcga cgggacttac gaggccttcg
     4321 cgccttattg ggcgctgagt ctcgcgataa gaccggaggc catccgagct caattcgaat
     4381 atcttgaccc gaaagaattt cccaacgcaa agaacgccga tcctaaagat ttctttgata
     4441 attcttttgt cgattcgctg gcgaagtcag gatttttgaa gcaactcggg atgaagtgaa
     4501 tttgtcatga cgatactttc gcgatcgcac agtttgagga aaggcgtgaa cagagcgggc
     4561 cttgatgcga cgagcagaag agatatcgtc tatctcgctc tcgcggcttt tttcgtaacc
     4621 aatgccatcc tgggtgaact caccggcggt aagctattta ccttagggcc gtttaccatg
     4681 agcatcggcg tgattccgtg gccggtggtg tttatcacta cggacctgat caatgaatac
     4741 ttcgggcgcg acggcgtgcg caggctgaca ttgacgacca ttggccttat cgcctacgct
     4801 tttgtcgtac tctacctttc gattcaagtt ccggcggcat cgttttcacc ggttaacgac
     4861 acgcagttcc aggccgtttt cggtcagtct ctttggatca tcgtcggcag catagttgcc
     4921 tttggcataa gccaaatggt cgatgtcggc gtgttctggc tggtgcggca cagaaccggc
     4981 ggcaaacatc tttggctgcg cgcgacaggg tccactctgg tttcgcaact catcgatagc
     5041 atcgtcatca ttggaatcgc gttttggctg ccagggaagg taaagactga ggagtttttt
     5101 atagttgccg gaaccaacta ttcttataag atgatgatcg ccgtcggggt cacgccctta
     5161 ctttatctgg gtcattcgat catcgatcgt tacctaggca tcgaagaatc ccatcgtctg
     5221 atcgaaagct cggctcaagc cgacgcagag ctggccgtgg cggaacctaa ttgacgatca
     5281 tcaaatgaca atcgtgaggc agctttttca tcagcccttt aagtacatga cctcgcagca
     5341 tgtgatagaa ggccgagcgt tgagaggctc caataagaac ggcatcgaca tcaagtgcct
     5401 tggctgcgtt ggcaatcgcc tccggggcat tgtaagaaac gctccagatg gggatcgcct
     5461 cgagcccttt gccgtgaatc tcctgcaagg cggaacgtaa ggtaatcacc ccttcatcac
     5521 tgggccggtg cggcgtatcg ccggcaaaga gacccggcca ttcctcgacg tacacacagt
     5581 atagggcgct ttcgcccatg cctttggcgc gccgaatgcc ttcctgaact aggcgcgggt
     5641 tttctccgcg aacggccaga agcgtcgatg acggatacag atttttgagt tcaacagcat
     5701 cgcctaaagt caccagaccc cttaattctt cgtctacgag agcttctgat gccatatagg
     5761 ctcgcgcctg aaggcgctgg atccaaggaa ctttatgaag cagatccaaa aaccacgcgc
     5821 ggcggacgcc tacggctatg ctcatcccga tcgccgttaa gacgcccccg aacaatgtgg
     5881 ccagttcttt ttcgacgaga ttgatacacc aggcaaccag gatcacgccg gtggttaaaa
     5941 ctccgaccca gaaggcaaaa tcgcgccgcc cgagccgcca acgaatgaca tcgaggctaa
     6001 gagatgaaaa gagaaacgcg ccaagcagtc cgaacgcata catttcaccc aacagggcca
     6061 actcgccttc agttgcgaga atcaccgcaa ccgggacaat ggtcgctata gcaatagcga
     6121 tatgaggact acgaaaagtc gcgcttcgca gggcgatggc ccgcggcagg aaaccaagat
     6181 tagtcagcgc caggaaaata tggtaggcgc cgatgatggc agtattggcc gcgaacaaca
     6241 gcaaggtcga cgcgctgagt acgacggcaa tgttaagccc aagtcctccc gagaccagcg
     6301 caacttcgga aatgaaacgt tcgctctgcg tcgcctttat ttcagcagga agcaaattga
     6361 ccgacaacat ggtcagcaaa ggcgaggtga tcacggtagt aatgaccact ccgatcatcg
     6421 cccatttggc agtgcgcctg agcggaaagc gcatattcgg actcagttgg ctgatacttt
     6481 ccaaacccga gaacgccagc caagccgcgc caaacccaac tagaagttct cgaaagctga
     6541 ggttctgtct tggcgcaaag ctctgcaccg catgccacaa ttgggcaggt gagaactgcc
     6601 agaatgacac cgcgatcaca atcagatcga cgcttagtgc tgctatcgcc ataaccagcg
     6661 ccacagtggc gctttcgcga atacccacta tattcaaaag agccaaggcc aacagaccga
     6721 tgcacgcaaa cagaacaatg ttggcatcga tcaagtggac tatactgccg atgtagtgga
     6781 atccagaaac cgccgagata gcagctgtca gaaaataact ggtggtgatc aacatgccgc
     6841 cgagacagcc ccacatcggt cccaaagctt tggtggtgat ggacacgacg ccgccgcctt
     6901 cgggattgcg ccagctgatc tcgatatatt tcagcgccag cggaatgaaa ccgatggtgg
     6961 ttagtaagac aaagaacggg atattatcgc cgacattgcc gtagagaatt cccggaacat
     7021 agtagaccga ggtgccgatg tcgcagaaca cgacagccca ggcgagaagc caggtgagtt
     7081 gcctggtatc tttggtatgc tgcatcagac cagggacttt tgtgattgcg gtttcagatt
     7141 aggcaaatga tgagctgcgg ctcttgcatt gacgtcgtcg ttcgacctat catacgtatg
     7201 ttggccgacg tcggtatgcc cctacctgta caatatttcc agaagctcgt cgagagttgt
     7261 cctgatatca tcatctccgt cgacaaatac ggcgtcatta cattctataa cgatggcgcc
     7321 aggcaaaatc tcgggttttc ccacgaggaa gtgcttggca aaagcgtttt ggcgatctat
     7381 ccgacattgg atgaggcgcg ccgggtcatg accgcgttgc gcgccgggtc cggcgaaggt
     7441 caaggcagga tcaggagttt tgaaactaca ttcaaaacaa aaaagggtga gctgattccc
     7501 gtcgccatct ccgcgtccat catttacgac gacaatggcc aggagattgg ctctatcggc
     7561 tttgccaaag atctaaggga cattcgccgg cgcgatcaac tcgtcacttt ggctgagatc
     7621 gccattggtg tatcgcacga agtcaacaat tcactggaag tgttggtaaa ccaggtagaa
     7681 ctcttgcaga agtatgttga acgagtcgcc agcgacgagg attacattgt cgaatcagac
     7741 cgtttggcat cggtcgcctc gcaggtgaag aagatccagg acatcactgc gcgaattagc
     7801 caaatggccg atgagggcga gtacggaacc aaggagtatc tatcaggcaa gatgatgacc
     7861 gatctgcggc ccgggctgaa cgggaaaggt tgtgagcgcc caaaacctga cgaacgctat
     7921 ccattacgcg gcctcaagat cttaatcgtc gatgatgatg ccgggatttg ccagtcgttg
     7981 agagatctgt tggaggcgga ggattgcgag gtgcaaaccg ctccatcggg tgtttatgcg
     8041 atgcagtggt tggagcgcag ccagttcgat ttagtcctga gcgatgttgt catgccggat
     8101 atagacggtt accagctcta tcaacgtgtg aagaaggatg cgccggagct tccggttatc
     8161 ttaatgacgg ctttcaatta cgataaggat catattatca agcggagctg tcttgaagga
     8221 ttgcaggggg tgattttcaa gaaaccggtg aatccgatgc tgctcaagaa ggcgctgctc
     8281 cagcaatgta gacctgaaag agttcatcaa caggccgctg ccggttcctc gcctcaaaca
     8341 acgcctccgc ccgattcaaa acgtttacgc tgacatgagc aaagaagatt tctacaacaa
     8401 gggtatggaa ctgtttatgg aggaaaactt cgacgcggca attgcagcct ttcagcaggc
     8461 gctggccgag gatgccaaat acacggatgc cttgcatgcg ctagccatga cctactcgca
     8521 tcaagaaaaa ctggacgacg ccatcgaaac cggcaagaaa ctcatcgagg ttgcgccgga
     8581 cgacgagcta gctcacacca gcctgtcgat attttaccag caaaaaggaa tgatcgcgga
     8641 ggccgagcac attgctgcgc aagctcggac gctaggctgg aagcgacagt tgtctcagaa
     8701 aaatgagcca caaaaaagct aaccattgat cattgttctc
//
LOCUS       DASUVS010000207        14621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_304047, whole genome shotgun sequence.
ACCESSION   DASUVS010000207 DASUVS010000000
VERSION     DASUVS010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14621
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304047"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VF182_11545"
     CDS             complement(<1..1148)
                     /locus_tag="VF182_11545"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HEX6437757.1"
                     /translation="MTTVENLVSRYREPLETYPLDLASDWKKMHPDKKLVGCYPVYSP
                     VELIHAAGMLPVGVIGAGNRLEIAHADSRFQSFICSIVKSTLELGLTDKLKFLDGIVF
                     HSICDPARNLGSVFTRNFPDLMVEYIHFPQNMTSPHTIDYLMAEYRRIGASYEKLSGK
                     KITDDGLSQSIALYNRQRRLIRELYAIRRESPQNLTTVECYVLTRVGTLLPPEEHIAI
                     LEEAIPQLRQRNQKPKDRIKVVLEGSFCEQPPIELLDGLEAAGCYILDDDFLTGWRWF
                     PEDISNEGDPLRNLANAYLNRSVYSGVKHDTREPKSKHLIDKFRETGAAAVLILAAKF
                     CEPALFDYALYKRALEKEGIPHLCLEFEEKMWIFDKARTEVETFVESML"
     gene            complement(1761..3980)
                     /locus_tag="VF182_11550"
     CDS             complement(1761..3980)
                     /locus_tag="VF182_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX6437758.1"
                     /translation="MIPVHIAGVGMTRFGKSPNTLVEIMRDAAVSALAASKIQDLDAI
                     YIAVMNPEEYTGDANIASHLAEVLGLRGIPAVRVETASSAGAAAVHVAFQGVASGYYR
                     HVLVVGGEKMTHLTTSASTRILAEVIDAQERNCGATMPALAAMITARYRKKYRLSEST
                     LARVLCAVAMKNHANGVHNPCAQFRQVITQEKYFASKFVATPLRLYDCAPMSDGGAAI
                     ILTGERTDVTVCGIGQGTGPVSLRERETFTCFPATQIAARRAYQMAGISPREIDCAEV
                     HDAFTPFEIIGTEDLGFFPAGKGVEAAASGRTAADGNLPINASGGLKSRGHPVGASGV
                     AQIVELVHMLREQSTAKLSRAPHRCLAQSIGGLASNNFVTILERSKGVTQLLCSSIAP
                     PSRQAELPRVEKKHPALPIESEGVIETFTIVYTTPDGFLPPLALAMIRDRKGARIMAQ
                     GEDISHLQIGREVCLHQVAGVFYFTVKNHLKKVRAAMRDFCAVRAIRNDPNTPTERIE
                     LIAKRTTCGSEKQFHSMIKLVADELRDAIAAGKIAPGSRMAEGQLALSLKVGRVPLRD
                     ALRRLESEGYITAISHGQFMVSQLTLEDAEEYYSIAGSLEGLAARLAVERGSAEELAR
                     LRDLHRRLKLAYREKNLEQYFEANRQFHRFIAQMSRNDRLYQLISEMRRGLRKARLLA
                     LQVPQRADYSMREHDQILDAFLKRNPDLAESTVLKHLANQLAAIKEILQSSEGSSHA"
     gene            complement(3985..4995)
                     /locus_tag="VF182_11555"
     CDS             complement(3985..4995)
                     /locus_tag="VF182_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437759.1"
                     /translation="MSQTIVVRYDPRLIEEAVFLALRNHPDGRQYQKEREHVYEIDDA
                     REQDRLFGELNQEWLVRLGLNKAIEQSLAEQPVISSTTSSCFVGRVAQAKEEGAELFV
                     GPDEQLDAIACRIVSILLRPESMLQREVLLTLLRHELFHIADMLDPAFAYEPALPKAE
                     GGPTYDTLITNRYRALWDVTINGRMTRRGWLAATAREQQLSDFRNAFPMLEENHEEFF
                     KQFFDAEQPRHAELVAFAFDPRAASGVGIHSSAPGTHCPLCRFPTHAYEPEPEGLDAA
                     VLGAIADDFPNWTPSHGLCLHCADLYRGRQMSMDALRLLPGWNQGIASMSRVTGRPDP
                     AD"
     gene            complement(4992..5792)
                     /locus_tag="VF182_11560"
     CDS             complement(4992..5792)
                     /locus_tag="VF182_11560"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEX6437760.1"
                     /translation="MTLNELHERTDDIDIAYCRATLYSALALGFRPPTEEAIARLVTA
                     QNAAALGEAAAIFDSDGKLGVVSLVEAVAHAGSAGVAGLVSSYRALFGHTVRGPIPPY
                     ETEYGNEALFQQPQEMSDLMGFYRAFGLTVKVGGHERCDHISCECEFLSFLALKEAYA
                     LEHGDLEMVEESRKATRLFLRDHLGRFLPAFASKLQREDPTGFYGMLVKLCLAFIEVE
                     SNRFGVPRGAASLGLRPADDDRVPMACGSGAECAALPGACAPEGADCV"
     gene            complement(5912..7177)
                     /locus_tag="VF182_11565"
     CDS             complement(5912..7177)
                     /locus_tag="VF182_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6437761.1"
                     /translation="MPNVHNWQIGREMNYPYTQAFPKRQFAFVFNINRCIACQSCTMA
                     CKSTWTFSKGQEHMWWANVETKPYGGYPQFWDAKILDLLEKANPGNQQWSGQPSADKK
                     KPYGQFDGKTIFEAQNALTPDSARVLGYLPTDEEWNSPNIFEDNPVGKKGVRNELDKE
                     GAALPEHKTWFFYLARICNHCSYPACLAACPRKAIYKRPEDGIVLIDQKECRGYRKCV
                     EACPYKKTMYRGNTRVSEKCVACYPRIEGKDPESGGQPMETRCMAACIGQIRMQGLVK
                     MNNDGTWAEDRYNPLYYMVHVAKVALPLYPQFGTEPNGYYIPPRWVPRDYLKQMFGPG
                     VDEAIERYSYPDRELLAVLQLFRRSNLIIDRYEIKEGEKIYEADLRGRKITMYNDTVI
                     AYGQNGKEIFRTQVEEPVHVRANKHANSI"
     gene            complement(7235..7594)
                     /locus_tag="VF182_11570"
     CDS             complement(7235..7594)
                     /locus_tag="VF182_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437762.1"
                     /translation="MPFNDPDPTDPNILVGVMLPADAEATRDMAYVFAEEFARFGYTR
                     EQLLGLFKNPFYGGAHGAYRSLGEPETLSIIDECLKVWGNVKVRVQDVQAVQSVQPPP
                     SSSPARVEEGLGGSNKS"
     gene            complement(7748..11215)
                     /locus_tag="VF182_11575"
     CDS             complement(7748..11215)
                     /locus_tag="VF182_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6437763.1"
                     /translation="MNDAEARRMMNVTRRDFLKWMGLGTGAAAIGSGCLNYFSATDAG
                     DNPLARSINRDWEKIYRDQYKYDSSFDWVCSPNDTHACRVRAYVRNGVVTRLGSTYDY
                     QNYADLYGNKATVNWNPRQCAKGYTFHRVIYGPYRLRHPIVRKGWKAWVDAGFPELTP
                     ENKSRYMFDARGQDEFVQISWPDAFDSIAKALVAISTRYSGEEGKKRLLAQGYQPEMV
                     EEMGGAGTRTVKMRGGMGLLGVIGKYGMYRLNNSLALLDAKVRGVGPDQARAGRNFSN
                     YTWHGDQAPGHPWVHGLQASECDFNDLRFSKLIIMDGKNLVENKLTDSHWFIETMERG
                     AKIVVIAPEYGPPSTKADYWIPIRPQTDAALWLGITKLMIDKKWYDEAFVKRFTDFPL
                     LVRTDNLRRLRAAEVFPNYKTTLSPDGPSMKIQGLTAEQHAKLGDYVIWDSKSKAPGA
                     INRDDVGEAMIKKGVDPALEGTFKVKLVDGKEVEVATLWTLYQTHLKDYDIETVAEIT
                     QAPKEMIEQLAQDIATMKPVAIHQGEGINHWFHATEMNRASYLPLMLTGNIGKPGAGC
                     HTWAGNYKAALFQGSPWTGPGFKGWVAEDPFELNLDPKAHGKEIHAHAYTKDEEPAYW
                     NHGDLALIVNTPKFGRKNFTGKTHMPTPTKALLFNNVNLINNAKWAYGMIKNVNPNVE
                     MIVSMDIQMTASIEYADVALPANSWLEFEGLEITASCSNPFLQIWKGGIPPVFDSKDD
                     LDIIAGIANALANATGDKRFRDCFAFAAADKRGIYIQRLLDTCTTTAGYKLSDIMAGK
                     YGPPGGCLLNFRTYPRIPFYEQVHDSEPFHTDTGRMHAYADVGEAIEYGENFIVHREG
                     PEATPYLPNVIVSSNPLVRPEDYGIAANAEHWDDRTVRNIKMPWSKVKQTKNFLWEKG
                     FQFYCLTPKTRHRVHSGWSNVDWHMLMDSNFGDPYRLDKRAPCVGEHQLHMNPQAARD
                     LSINDGDYVYLDANPADRPYLGAKPGDPFYRVSRCMLRVTYNRAYPYNIVMMKHAPFI
                     ATEKSVKAHETRPDGRALSENTGYQANLRYGSQQSVTRNWHMPMHQTDTLFHKTKAFM
                     GFIFGGEADNHAVNTVPKETLVRVTKAEDGGMGGKGIWKPATSGFSPDNENELMKRYL
                     AGDLTKVKA"
     gene            complement(11384..12967)
                     /locus_tag="VF182_11580"
     CDS             complement(11384..12967)
                     /locus_tag="VF182_11580"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HEX6437764.1"
                     /translation="MIKRLLVVAVLGFVVLLITVVSKTAEIKPPPSTPKLIASGKQIY
                     AQQCAACHGNEGRADGEAAYLLYPKPRDFVAAKYRLVSTWDRVPTDQDLFDTISRGMP
                     GSAMPSWEHLPADQRWGLVYYIKSLAEKPLEIKAQKMPAADGSGGEGIIAVPKAPPFD
                     AAARARALELYKDACASCHGDTARGDGTQEQKDDEGFPTRPRDLTAGVFKGSPDPIAL
                     YRHIVAGIPGSPMPMSDWAYGDDAWHLVNYVRSLSSDTQRARVEMKKFTIEALRVATL
                     PDHPEASVWRSARPVNLHLMPLWWRSDRPEEVTVRAIHDGKDIAILMVWHDDTSDQSA
                     LRTQDFRDAAAIQFSLSANPPFFAMGANGAPVNIWMWKSERQADLDVAYQDLDRAYPN
                     IGIDSYPNLTRSAVEQPTRRAVTLQSDPAFVTGWGAGNIVSDPQRKSAVEDLSAQGFG
                     TLRARPMIEQKIQARGVYGQGSYSVMFRRTLAATGKGAVALTADSTVPVAFAVWNGSA
                     GDRDGKKSVTIWQDLKIGK"
     gene            13415..13759
                     /locus_tag="VF182_11585"
     CDS             13415..13759
                     /locus_tag="VF182_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6437765.1"
                     /translation="MARLPKAYEDFKKAYPKIWQDYDRLGAAVHDSGPLDKKNRELIK
                     LGMAIGARLEGAVHSHTRRALEAGATAEEIYHVVLLSLTTLGFPQTVAAMTWVEDDLK
                     DDKHRERKGDNR"
     gene            13772..14281
                     /locus_tag="VF182_11590"
     CDS             13772..14281
                     /locus_tag="VF182_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX6437766.1"
                     /translation="MHVYYLISVWLHIMAAIVWVGGTIFLVIVLVPAIRRPQFAVVAS
                     PLIRFTALRFRWVGWVCFGVFVSTGIVNLAARGIRWQELQEAVFWRGSFGRTLAVKLI
                     LVAAILAISGFHDFFLGPRASAAWESNPASAETLRLRRQAVKLGRLNLLLALTAIVLG
                     VMLVRGVPW"
BASE COUNT         2980 a         4090 c         4138 g         3413 t
ORIGIN      
        1 agcattgact cgacgaaggt ctcgacctcg gtgcgcgcct tatcgaagat ccacatcttt
       61 tcttcaaact cgaggcagag gtgtggaatg ccttccttct ctagcgccct tttgtagaga
      121 gcgtaatcga ataacgccgg ctcgcagaat ttcgcggcga ggatcaggac tgcggccgcg
      181 ccggtttctc taaatttgtc gatcaaatgt ttcgatttgg gctcgcgcgt atcgtgtttc
      241 acgcctgagt aaacactgcg gttgagatag gcgttagcga ggttgcgcag gggatcgcct
      301 tcatttgaaa tatcctcggg aaaccagcgc cagccggtaa gaaaatcatc gtccaggata
      361 tagcagccgg cggcctcgag tccgtcgagc aattcgatgg gcggctgctc gcagaacgat
      421 ccttccaaga ccaccttgat ccggtccttg ggcttttgat tgcgctggcg cagttgcggg
      481 atcgcctcct caagaatcgc gatgtgttcc tcgggcggca gtaacgtgcc gacccgcgtc
      541 aagacgtaac attcgacggt ggtaagattc tgtgggctct ctcggcgaat agcatacagc
      601 tcccggatca ggcgtcgctg tcgattgtaa agcgcgatgc tctggctgag cccatcgtcg
      661 gtgatctttt tgccggagag cttttcgtaa ctggcgccga tccttcggta ctccgccatc
      721 agataatcaa tcgtgtgcgg cgaagtcata ttctgcggaa agtgaatgta ctccaccatt
      781 agatcgggaa aatttcgcgt gaacacgctg ccgaggttgc gcgccggatc acagatcgag
      841 tgaaaaacga tgccgtcgag aaatttgagt ttgtcggtca gaccaagttc cagagtgctc
      901 ttgacgatgg aacagataaa cgattgaaaa cgtgagtcgg catgggcgat ctctagccgg
      961 ttgccggcgc caatgacgcc gacaggcagc attcccgcgg catggataag ttcaacgggg
     1021 ctatagaccg gatagcatcc gacaagcttt ttatcgggat gcattttttt ccagtctgat
     1081 gcgaggtcga gagggtaagt ctcaagaggc tcgcgatagc gggaaacaag attttcgaca
     1141 gttgtcatgg gtgacctctt actcgcttta ctccgcgttt tacacgcaat accgcaatag
     1201 ggatgccacc agtattcgac tggttgctct ggattttcga tcgaatcttc gcaaccatcg
     1261 gaaagctgcc tccgatagtt ctcaattctc agaatgaaga tggccgccgc tctcctggcc
     1321 gctcccatga gacttgcctt gttactttcg tcgatagttt ggtgcgaaca tcgctcgcga
     1381 atagacgagc atcctcatcg cgcgtgcgcg aagatgcata gccgatgctg atcttggggg
     1441 gagccttccg ttcgacgaga tcggcgagtg gctcgttgat gctattgggc ttgacgtcgc
     1501 gaccgctatc cagaagcgcg ggcggactat atgaaatcga gatcgacggc ttttaggata
     1561 tctgctgaac ttcgcttcta gttattaaca aagatctcaa gcggcctcac gcgcaccgac
     1621 cctgctgatt gagaaacttc tgcgtcagct tgaaaccggc acgaaatgct cgcagcccat
     1681 gatgacgtcc gcccagctta gcgcttgcgc tgaacagtgg tgatttgtga aggtctgcct
     1741 ccttgaaaac ttccacgaag ctatgcatgg cttgagccct cagacgattg caatatttct
     1801 ttgatggctg ccagctgatt ggccaggtgt tttagaacgg tgctctcggc gagatctgga
     1861 ttgcgcttga gaaaggcatc gaggatctgg tcgtgttcgc gcatcgaata atccgcccgc
     1921 tgaggcacct gcagcgcaag caggcgagct ttgcgtaggc cccgacgcat ttcactaatc
     1981 agctgataga gccggtcgtt tctgctcatc tgagcgatga agcgatgaaa ctggcggttg
     2041 gcttcaaagt attgctccaa atttttttcc cgataggcca gcttgagccg tcggtgcaag
     2101 tctctaagcc gagcaagctc ttcggcgctg cctctctcca cggctaatcg tgcggctaat
     2161 ccttcgagcg agccggcgat cgaatagtat tcctccgcgt cttcaagagt cagttggctc
     2221 accatgaatt ggccatggga gatagccgtt atgtaccctt ccgactcgag acgccgcaga
     2281 gcgtcccgca acggaacccg gccaactttg agcgataacg cgagctgtcc ttcggccata
     2341 cgactgccag gcgcaatctt accggcggca atcgcgtcgc gaagctcgtc ggcaaccagc
     2401 ttgatcatcg aatgaaactg tttctcggaa ccgcatgttg ttctcttggc gataagctcg
     2461 atcctttcag tcggcgtatt aggatcgttc cgaatcgccc gcacggcgca aaaatctcgc
     2521 atggcggcac gaaccttctt taagtgattc ttcacggtga agtagaaaac tcctgcgact
     2581 tgatgcaagc aaacctcgcg accaatctgc aagtggctga tgtcttcacc ttgcgccatg
     2641 attcgcgctc ctttacggtc gcggatcatg gccaaagcca acggcggcaa gaagccgtcc
     2701 ggggtcgtgt aaacaatcgt aaaggtctcg atcacgccct cgctttctat agggagagcg
     2761 gggtgtttct tctccacgcg aggcagctca gcctggcggc tcggcggtgc tatcgaagaa
     2821 cagaggagct gcgtgactcc tttcgagcgt tcgaggatgg taacgaaatt attggaagcg
     2881 agcccgccga tgctttgcgc caggcagcga tgcggcgcgc ggctaagctt cgccgtcgat
     2941 tgttctcgca gcatatgcac gagctcaacg atctgagcca cgccgctagc gcccacggga
     3001 tggccacgcg acttgaggcc cccggaggcg ttgatcggca gattcccatc cgccgctgtc
     3061 cgtccggacg cggcggcctc cactccttta ccggccggaa agaagccgag gtcttccgtg
     3121 ccgatgatct cgaaaggcgt aaaggcgtcg tgaacctccg cgcagtcgat ttcccggggc
     3181 gaaatgccgg ccatttgata agctctcctg gcggcgattt gggtcgccgg gaaacaagtg
     3241 aacgtttccc gctcgcgcaa actcacgggg ccggtaccct ggccgattcc gcagacggtc
     3301 acgtcggtgc gctcgccggt aagaataatc gcggcgccgc cgtcgctcat aggcgcgcaa
     3361 tcgtacagtc gtaaaggggt tgccacgaat ttactggcaa aatatttctc ttgagtaatg
     3421 acttggcgaa actgagcgca aggattatga actccattgg cgtgattctt catcgcaacc
     3481 gcgcataaaa cccgcgccag agtagattct gacagacggt attttttgcg gtacctggcg
     3541 gttatcatgg ccgcgagcgc gggcatggtg gcgccgcaat tgcgctcttg cgcatcgatg
     3601 acttctgcga gaattcttgt gcttgcgctg gtggtcagat gggtcatctt ttcgcccccg
     3661 accaccaata cgtgacggta atagccggac gcgacgccct ggaatgcgac atgaaccgct
     3721 gccgcgccag ccgaagacgc cgtttccact ctcactgccg ggattcccct gagtccgagc
     3781 acttcggcca gatgtgaagc gatattggcg tcgccggtgt attcttccgg gttcatcacc
     3841 gcgatgtaga tggcatcgag atcttggatc ttcgatgccg ctagcgcgct cacggcggca
     3901 tcgcgcatga tctcgaccag cgtgttcggg ctcttgccga accgggtcat gccgacacct
     3961 gcgatgtgaa cgggaatcat ccgcctaatc ggccgggtcg ggacgccccg tcacccggct
     4021 catgctcgcg atgccctggt tccatcccgg cagaagtctc aaggcgtcca tcgacatttg
     4081 tcggccgcgg taaagatcag cgcagtgcaa acacagtcca tgcgagggtg tccagttcgg
     4141 gaaatcgtct gcgatcgcgc cgagcaccgc ggcgtcgaga ccttcgggtt ctggctcata
     4201 ggcatgagtc gggaatctac acagaggaca atgagtgccg ggagcggaac tgtgaatccc
     4261 aactccgctg gcggctctcg ggtcaaaggc gaacgcgaca agctcagcgt gcctaggctg
     4321 ctcggcgtca aagaactgtt taaaaaactc ttcgtggttt tcttccaaca tcggaaaagc
     4381 gttccgaaaa tcactcagct gctgctcccg ggcggttgcc gcgagccagc cgcggcgggt
     4441 catccggccg ttgatcgtca catcccatag cgcgcgatag cggttggtaa tcagcgtgtc
     4501 gtaggtggga ccgccctccg cctttggcaa cgccggctcg taagcaaatg ccggatcgag
     4561 catgtcggcg atatgaaaca gttcgtggcg tagcaaagtc agtaatacct cccgctgtag
     4621 catcgattcc ggccggagca gaatgctcac gatgcggcag gcgattgcgt cgagctgctc
     4681 atccggaccg acaaaaagct cggcgccttc ttctttggct tgcgccaccc gaccgacgaa
     4741 acaactgctc gtcgtcgaag aaatcacggg ctgctcggcc aaagactgtt cgatcgcttt
     4801 gttcaggccg agtcgaacga gccattcctg attcaattcg ccgaataatc ggtcttgttc
     4861 ccgcgcatcg tcgatttcat agacgtgctc gcgctctttt tgatactgcc ttccatccgg
     4921 gtggttgcgc aaagctaaaa acaccgcctc ttcgatcaag cgcggatcgt agcgaaccac
     4981 gattgtctgg ctcatacgca gtcggctccc tctggcgcgc aggcgccggg caacgccgcg
     5041 cattccgcgc cgctgccgca ggccatcggt acccggtcat cgtcggccgg acgtaaacca
     5101 agacttgcgg cgccccgcgg cacgccgaaa cgattgctct ccacttcgat gaacgcaagg
     5161 caaagcttga ccagcatgcc gtaaaaccct gtcggatctt ctcgttgcag cttgctggca
     5221 aatgccggca gaaagcggcc aagatgatcg cgtagaaaca ggcgcgtagc cttgcgcgat
     5281 tcttccacca tctcaaggtc gccgtgctcc aaggcatagg cttccttaag ggccagaaaa
     5341 gatagaaact cgcattcaca gctaatgtga tcgcagcgtt cgtgcccacc aactttgacc
     5401 gtaagaccga aggcgcgata gaaacccatc aaatcgctca tctcctgcgg ctgttgaaaa
     5461 agcgcttcgt tgccgtactc ggtttcgtag ggcggaatcg gaccgcgcac ggtatggccg
     5521 aacagggctc tatacgagga tacgaggccg gctacgccgg ccgagcccgc atgagcgaca
     5581 gcctcgacga gagagacgac tcccagtttg ccgtcgctat cgaatatggc cgccgcttct
     5641 ccgagagccg ccgcgttttg cgcggtcaca agccgggcga tggcctcttc ggtcggcggc
     5701 cgaaagccca acgccagcgc gctgtaaaga gttgcgcgac agtaggcgat gtcaatatca
     5761 tccgtgcgtt cgtgcagttc attgagtgtc attggctacc tcgtcattgt aatggttaaa
     5821 tccttcaacg gttcaaacga tcgaaaactc cgaaacgttt tggaacggag cgacgcggtt
     5881 gaacggcttg aacgttttga acgtgttcca ttcatattga gttcgcatgc ttattcgctc
     5941 gcacgtgcac gggttcctcg acctgagtgc gaaaaatttc tttaccgttt tggccgtacg
     6001 cgatcacggt atcgttgtac atggtgattt tgcgcccgcg caggtcggcc tcgtaaattt
     6061 tctctccttc tttgatttcg tagcggtcga tgatgagatt cgaacggcga aagagctgta
     6121 gcacggcaag caactcgcga tcgggatagc tgtagcgttc gatggcttca tcgaccccgg
     6181 gaccgaacat ttgctttaaa taatcccgcg gcacccaacg cggcgggata taatagccat
     6241 tgggttcggt gccgaactgc gggtagagcg gcagggcgac tttggcgaca tgcaccatgt
     6301 agtagagcgg gttgtaacgg tcttccgccc aggtgccgtc gttattcatt ttgaccaatc
     6361 cctgcatccg gatctggccg atgcaagccg ccatgcagcg ggtctccatc ggctgtccgc
     6421 cgctttcggg atccttgcct tcgatgcgcg gatagcaagc gacacatttt tccgagacgc
     6481 gggtgttgcc gcgatacatg gtcttcttgt acgggcaggc ttcgacgcac ttgcgatagc
     6541 cgcggcactc tttctgatca atcaggacga tgccgtcctc gggacgttta tagatcgcct
     6601 tacgcgggca tgcggctagg caggctggat agctacaatg attgcagatg cgcgcgagat
     6661 agaaaaacca ggtcttatgc tcggggaggg cggctccttc tttgtcgagt tcgttgcgca
     6721 cacctttctt gcccaccgga ttgtcttcga aaatattcgg cgaattccac tcctcgtcgg
     6781 tcggcagata gcccagcacc cgggcgctgt ccggagtcag cgcgttttgt gcttcgaaaa
     6841 tagtcttgcc atcgaattgc ccatacggtt ttttcttatc ggcggatggt tggccggacc
     6901 attgttgatt gccggggtta gccttctcca atagatcgag aatttttgca tcccagaact
     6961 ggggatagcc gccgtacggt ttcgtttcga cgttggccca ccacatgtgc tcttggcctt
     7021 tgctgaacgt ccaggtggac ttgcaagcca tggtgcagct ctggcaggca atgcagcgat
     7081 tgatgttgaa cacaaaggcg aattgccgct tcggaaaagc ctgcgtgtag ggataattca
     7141 tctcccggcc gatctgccag ttgtggacgt taggcatctt cttactcgct cctctcactc
     7201 attcacgttc aaaccgttca agctgtctcc aaggtcagga tttgttcgaa cctccgagtc
     7261 cctcctccac gcgcgcgggg gaggacgaag gagggggttg aacgctttga actgcttgaa
     7321 cgtcttgaac tcttactttg acgtttcccc aaaccttcaa acattcgtcg atgatcgata
     7381 gagtctctgg ttccccaagc gatcggtagg ctccatgggc gccgccgtag aagggatttt
     7441 taaacagccc gagcaattgc tctcgcgtgt aaccgaagcg ggcgaactct tcggcgaaga
     7501 catacgccat gtcgcgcgtt gcttccgcgt cagcgggcag catgacgccg acaagtatgt
     7561 tcggatcagt ggggtcggga tcgttaaaag gcattttttt cgctcctatc gctcgcgttc
     7621 aaatcgttcc agccctcacc ccggatcgag tccggggcag gctctttttc ctcgctcgca
     7681 tcgcggggga ggacgaagga ggttgaaact ttcttggaac cgttggaacc actggaacgg
     7741 cttcgcttca cgctttcacc ttcgtcaaat ctccggccaa gtagcgcttc atcagctcgt
     7801 tttcgttgtc cggcgaaaaa ccgctggtgg ccggcttcca gatgcctttg ccgcccatgc
     7861 cgccgtcttc ggctttagtg actcgcacca gagtttcttt gggtacggtg ttgaccgcgt
     7921 ggttgtccgc ctcgccgccg aagataaatc ccatgaaagc cttggtcttg tgaaacaacg
     7981 tatcggtctg gtgcatcggc atgtgccaat tgcgtgtgac cgattgctgc gagccatagc
     8041 gcagattggc ctggtagccg gtgttctccg acaacgcccg cccatccggt ctggtctcgt
     8101 gcgccttgac gcttttttcc gtggcgataa agggcgcatg tttcatcatg acgatgttgt
     8161 acgggtaagc gcggttgtag gtgacgcgca gcatgcaacg cgatacccga tagaacggat
     8221 cgcccggttt tgcgcctagg tagggccggt cggcgggatt ggcatcgagg tagacatagt
     8281 ccccgtcgtt aatgctcaga tcgcgagccg cctgcgggtt catgtgcagt tgatgttcac
     8341 cgacgcacgg cgcgcgcttg tccagccgat agggatcgcc gaagttcgag tccatcagca
     8401 tatgccaatc gacgttggac cagccgctat gtacgcgatg gcgtgtcttc ggggtcaggc
     8461 aatagaactg gaagcctttc tcccaaagaa aatttttcgt ctgcttcacc ttgctccatg
     8521 gcatcttgat attgcgcacg gtgcggtcat cccaatgctc cgcgttggcc gctatcccgt
     8581 agtcctccgg ccgcaccagc ggattggagc tgacgatgac gttcggcaga tatggcgtcg
     8641 cttccggtcc ctcgcgatgc acgatgaagt tttcgccata ctcgatcgct tcacccacgt
     8701 cggcgtaggc gtgcatgcgc ccggtatcgg tatgaaacgg ttcgctgtca tgcacctgtt
     8761 cgtaaaacgg aatgcgcgga taggtgcgga aattgagcag gcagccgccc ggagggccat
     8821 atttgccggc catgatatca ctgagcttgt aacccgccgt ggtcgtacag gtgtcgagca
     8881 ggcgctggat atagatgcct cgtttgtcgg cagcggcaaa agcaaagcaa tcccgaaacc
     8941 gtttatcgcc ggtggcgttg gcgagcgcgt tggcgatgcc ggcgatgatg tcgaggtcat
     9001 ccttgctgtc gaataccggc ggaatgccgc ctttccagat ttgcaggaac ggattcgaac
     9061 aactcgcggt gatttccaat ccttcgaatt cgagccacga gttggccggc agggcaacat
     9121 ccgcatactc gatcgacgcg gtcatctgaa tgtccatcga aacgatcatt tcgacgttcg
     9181 gattgacatt cttgatcatg ccataggccc acttggcatt gttgatcagg ttgacgttat
     9241 tgaagagcaa agctttggtg ggtgtcggca tgtgcgtctt gccggtaaag ttcttgcggc
     9301 cgaatttcgg cgtgttcacg atcagagcca gatccccatg attccaataa gccggttctt
     9361 catcttttgt gtaggcgtgg gcgtgaatct ctttgccgtg ggcctttgga tcaaggttga
     9421 gctcgaaagg gtcttccgcc acccatccct tgaagccggg accagtccat ggcgaaccct
     9481 gaaagagcgc ggctttgtag ttgccggccc acgtgtggca accggcgccg ggcttgccga
     9541 tatttcccgt gagcatcaga ggcaaatagg atgcgcgatt catttccgtc gcatggaacc
     9601 agtgattgat gccttcgccc tggtggatcg ccaccggctt catggtcgcg atgtcctgcg
     9661 ccagttgctc gatcatctct ttaggcgcct gggttatttc ggccacggtc tcgatgtcat
     9721 agtctttcag atgggtctgg tagagggtcc aaagagtggc gacttccact tctttgccgt
     9781 ccacgagctt gaccttgaag gttccttcca gagccggatc aacacctttc ttgatcatcg
     9841 cttcaccgac atcatcgcgg ttgatcgcac cgggggcctt gctcttgctg tcccagatca
     9901 cgtaatcgcc gagcttagca tgctgttcag cagtcaaacc ctggatcttc atcgacgggc
     9961 catcgggtga aagcgtggtt ttgtaattcg ggaagacttc tgccgcgcgc agccgcctta
    10021 aattatcggt gcgcaccagc agcggaaaat cggtgaagcg tttgacgaat gcttcgtcgt
    10081 accacttctt gtcgatcatc aatttggtga taccgagcca aagcgctgca tcggtttgtg
    10141 gccggatggg aatccagtag tcggccttgg tggacggcgg gccgtattcc ggcgcgatca
    10201 ccacgatttt tgcgccgcgt tccatcgtct cgataaacca gtgcgaatca gtgagcttgt
    10261 tttcgaccag gtttttgccg tccatgatga tcagttttga aaagcgcagg tcgttgaaat
    10321 cacattcaga agcttgcagt ccgtgcaccc atggatggcc cggagcctga tcaccatgcc
    10381 aggtataatt cgagaaattg cggccggcgc gcgcctgatc ggggccgacc ccgcgaacct
    10441 tggcgtcgag cagcgccagc gagttgttaa gccgatacat gccgtatttg ccgatgacgc
    10501 caagcagccc catgccgccg cgcattttca cggtccgggt tccggcgcca cccatctcct
    10561 cgaccatttc cggttgatag ccttgggcga gcaagcgttt tttcccctcc tcgccgctat
    10621 aacgagttga gatcgccacc agcgctttgg cgatggagtc aaaggcgtcg ggccaggaaa
    10681 tttgcacaaa ctcatcctgg ccccgcgcat cgaacatgta tcgcgatttg ttttccggtg
    10741 tcagctcggg aaacccggca tcgacccagg ccttccagcc cttgcgcacg atcggatgcc
    10801 gcaaccggta cggcccatag atgacacgat gaaaggtgta acccttggcg cactggcgag
    10861 gattccaatt gaccgttgcc ttattgccgt agagatcggc gtagttttga taatcgtaag
    10921 tgctgcccag ccgtgtcacg acgccattgc gcacgtaagc ccgcacgcgg caagcatgag
    10981 tgtcgttggg cgagcagacc cagtcgaaac tgctatcgta tttgtattga tcccgataaa
    11041 tcttctccca atcccgattg attgatcgcg caagaggatt gtcgccggca tctgtcgcgc
    11101 tgaaataatt caaacagccg gaaccgatgg ccgctgctcc ggtccccaaa cccatccatt
    11161 tgaggaagtc gcggcgtgtt acattcatca tccgtcttgc ttcagcgtcg ttcattctgg
    11221 tctctcctct cgtcccccgc gttccaatcg ttcaagccgt taatcgcttc ctccgttcca
    11281 agccgtttcg aaagcttcgt tcgatttcct ctcctccccc gcgcccggga gaatacagga
    11341 gggggttgag cgacttgaac gttttgaacg agttgaacgg tattcatttt ccaattttca
    11401 gatcctgcca aatggtcact gatttcttgc cgtcacgatc gccggcgctg ccattccaaa
    11461 ccgcaaaggc gacgggcacg gtggaatccg ctgtcagggc cacggctccc ttgccggtgg
    11521 ctgccagcgt tcgacggaac ataaccgaat aggaaccctg cccgtagact ccgcgtgcct
    11581 gaatcttttg ttcgatcatc ggccttgcgc gcagcgtgcc gaatccctgc gcggaaaggt
    11641 cttcgaccgc gctctttcgc tgcggatcgg agacgatatt cccagcgccc catccggtga
    11701 caaatgcggg atccgattgc aatgtcacag cgcggcgagt cggttgctcc accgccgagc
    11761 gcgtcaagtt gggatacgaa tcgatgccga tattgggata agctctgtca aggtcctggt
    11821 aagcaacatc caaatcggcc tgccgttcgg atttccacat ccagatattg accggcgctc
    11881 cattcgcgcc catggcgaaa aatggcggat tagcactcaa ggaaaactgg atggccgctg
    11941 cgtcgcggaa atcctgggtt cgaagggcgc tctgatcact ggtatcgtcg tgccagacca
    12001 taagaatagc gatgtctttg ccatcgtgta tggcccgcac ggtgacttct tccggtctgt
    12061 cgctgcgcca ccagagcggc atcaggtgca ggtttaccgg cctcgcgctg cgccagacac
    12121 ttgcctcggg atgatcggga agagtggcga cgcgcaaagc ttcgatggtg aatttcttca
    12181 tctcgacccg cgcgcgttgg gtgtcgctgg agagcgagcg cacgtagttc accagatgcc
    12241 atgcgtcgtc gccataagcc caatcgctca tcggcatcgg gcttcccgga attcccgcaa
    12301 cgatgtggcg ataaagcgcg atcggatcgg ggctgccttt gaaaacaccg gcggtcagat
    12361 cacgcggccg ggtgggaaac ccttcgtcgt ctttttgctc ttgcgtgcca tcgcctcttg
    12421 cggtgtcgcc gtggcacgag gcgcaagcgt ccttatatag ctccagggcg cgcgcccttg
    12481 ccgcggcgtc gaaaggcggc gctttgggaa cggcgatgat gccttcgccg ccgcttccat
    12541 cggctgcggg cattttctgc gctttgatct cgagcggttt ttcggcaagc gacttgatgt
    12601 aataaaccag cccccatcgc tgatccgccg gcaagtgctc ccaggagggc atcgccgagc
    12661 cgggcatgcc gcgcgaaatc gtatcgaaga gatcttgatc agtcggcacc cgatcccagg
    12721 tagagaccaa ccgatatttc gcggcaacga aatcacgcgg cttgggataa agcagataag
    12781 cggcctcgcc gtcagcccgg ccctcattgc cgtggcaagc agcgcactgt tgcgcataga
    12841 tttgttttcc cgaggcaatc agcttgggtg tggacggcgg cggctttatc tccgcagtct
    12901 tactgaccac agtaatcagc agaaccacaa acccgagaac cgcgactaca agaagacgtt
    12961 tgatcacttg gcctccagat ttttcagtga agaattgcga gcgattcgaa tactaagcga
    13021 tttgaaacgc cggcgcgccg atcgtccgtt cacctggttc gatctcatct ctcgataaag
    13081 tttttgccct agtcatcgcg gtttctcaca tgagcaaagc gaaggccatg aaacgttccc
    13141 tgtgaaaagg gggacgacag atcgaggatc tttccgactt ttgagaagct gaccagctag
    13201 ctttagcagt gatgggaatc cacgcgccgt tgcagaacgc cgaaccgcaa gttccgcgtc
    13261 caagagctgc tggtgtttgt agagatttcc tagcggactg aaaacaagaa agcgggatgc
    13321 aaagctggtt gaccgcgtgt gtcaaacgca tataacaatg cagggaaaat attttgttgg
    13381 gcaagattat tttgttgggc aagaaggagg cgctatggct cgattaccaa aggcctatga
    13441 agatttcaaa aaagcatatc cgaaaatctg gcaggattat gatcgattgg gtgccgcggt
    13501 tcacgatagc ggaccgctgg acaaaaaaaa ccgcgagtta atcaagcttg gcatggcgat
    13561 cggagcccgg ttggaaggcg ccgtgcattc acacacccgc agggcgctcg aggctggcgc
    13621 caccgctgaa gagatctacc acgtggttct gcttagtctc actaccttag gttttcccca
    13681 aaccgtcgca gcgatgacct gggtcgaaga cgatctgaaa gatgacaaac accgcgagag
    13741 aaaaggtgat aatcgatgag ctgatgcgac gatgcatgtt tattatttga tctcggtatg
    13801 gttgcacatc atggccgcga tcgtatgggt gggcggcacg atttttctcg tcatcgtgct
    13861 cgttcctgcc attcggcggc cgcagtttgc cgttgtcgcc tcgccgctga ttcgcttcac
    13921 ggcgcttaga tttcgctggg tcggctgggt ttgttttggt gtatttgtct ctaccggtat
    13981 tgtgaacctc gccgcccgcg gcatacgttg gcaagaactg caggaggcgg ttttttggcg
    14041 aggctctttt ggacgcacgc ttgccgtaaa gctgatactc gtcgcggcga ttctggcgat
    14101 cagtggtttt cacgattttt ttttagggcc tcgagcgtct gcggcgtggg aatctaatcc
    14161 cgcttcagcc gagacacttc gactgaggcg ccaagcggta aaattgggcc ggctcaatct
    14221 gctgctggca ttaaccgcaa ttgtcctggg cgtcatgctg gtgcgcggcg tgccctggta
    14281 gagcaatgga attacgagct cgaaattcga aacgatttcg aatgaacagc cgaatgatgc
    14341 aaacaaatgg gccagtccac ggcgtgtaac gtaactgtca cttcgcggtg aatccgagaa
    14401 atccttatcg tgcttggaat tttaaatttc gatattcgga tttcctctga gtcgcgctgt
    14461 cacaacgtta ttgaacgatg aagttaaatc caaaatgcgg ccggttcgtc ctcatgggtt
    14521 tagctgggct ctcactgcta gccggcctgt gggccggcat cggtcggatg ggctggttcc
    14581 tgccggtgcc gaacagtcag tttatagtga tccacggacc g
//
LOCUS       DASUVS010000208        41253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_957886, whole genome shotgun sequence.
ACCESSION   DASUVS010000208 DASUVS010000000
VERSION     DASUVS010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 41253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41253
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957886"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VF182_11595"
     CDS             <1..796
                     /locus_tag="VF182_11595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6437767.1"
                     /translation="GLEVATAVGVDPAIVVGSQARVPFGIDEFHVAGGLRGAAVKLTR
                     CETIDVEVPAFAEVVIEGVTVPGERVADGPYGEYPGCYSEAKQAPVLKVTAITMRHNP
                     IWQTALTGMPVTENHTLIEYGNAAVVYREVRKVVPEVRGINMTPGGTFRHHVVVSIKK
                     RAENEGRNVILALLSMGIGLKQVIVVDDDIDPFDPMQVDWAMATRFQADKDAIVIPRI
                     ACSTLDPSCPEPRVTAGLGIDATAPMKDRWRFEKVEIPGVDKVSYI"
     gene            1028..1897
                     /locus_tag="VF182_11600"
     CDS             1028..1897
                     /locus_tag="VF182_11600"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6437768.1"
                     /translation="MRNFDYLEPTTVAEACAMLKQHGGEAKVFAGGAHVTILMKQGLY
                     QPKALVNIKKISGLKNISFDTTEGLTLGALVTHREIETSALVKEKLPVLSEAEREIAN
                     IRVRNMGTVGGNLASGEPLTDLSQIFIALDGTLRIAGPDGQRAIRVEELFLDFYTTSL
                     AEDEIITQVVLPPLSPHSGIDYIRFSSSSVVDKPSAGVAVRLTLDKEAVQVARIVLGC
                     VGPTPVRANKAEALLIGKILNAELTREAGALASQECSPIGDLRGSEEYKRAIVNALVR
                     RATDAAYRKSLNQ"
     gene            2046..2516
                     /locus_tag="VF182_11605"
     CDS             2046..2516
                     /locus_tag="VF182_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6437769.1"
                     /translation="MNRKTISMNVNGVERKVEVEARTTLLDLVREEFGLTGAKLGCDI
                     QVCGACTLLLDGKPVSACSVLAVDAEGHNVVTIEGLGSDSKLDPIQEAFMEFGALQCG
                     YCTSGFILTAKALLDECPKPSDEQIRNYLAGNFCRCGCYQEIIQAVRKVSGAAD"
     gene            2603..4927
                     /locus_tag="VF182_11610"
     CDS             2603..4927
                     /locus_tag="VF182_11610"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6437770.1"
                     /translation="MSTNEIGKSARRLDYESKISGRAMYLADMHMPGMCHGKILRSPL
                     PHARIKGIDISRALKVAGVVTIITRDDILHDQGIEPYYGPVFKDQTILAVDKVRHVGD
                     PVAAVAAATLDAAEEALRLIEVDYEELPPVLDVHEALNNGAVLVHDSIKLPSSGFADL
                     AEIKPVEGTNICTHFKLNRGEVEKGFAEADRIFEDTFTLPATQHSFLETHACIALAEA
                     GRITVWATTQNPFVVRTQLANIFKVPVSKVRVIVPYLGGGYGGKVYPKVEPITVALAQ
                     KARRPVRVVLSREEVFYTITKHAAVIRMKTGVKNDGTLIARECEIHLDTGAYAEIGPR
                     VAKKSGYTAAGPYRIPNLKIDSYSVYTNKPPAGAFRGFGVSQSAWAVESQMDIIASAL
                     QMDPLELRLKNGYVNGDRFVTEETLRSVGLKDCLEGVAKSIGWEDKTFEGSNVQKFND
                     GNLKRGKGLACMIKATITPSISCAAVKLNEDSSLSVYSGTVEMGQGSETVLAQIAGQE
                     LGIPLQRIQVLGVDTDVVPYDLTTSSSRSTFHMGKAVQLAARDIMRQLKDIVVQEYGV
                     PADQVSFADGKVRLPEGALDYAEIMFKRFGMQGGTLMGEGEVKTSVKDEYGEKSTSAF
                     WFLAAGAAEVEVDVETGKFKLLKYATAVDVGKALNPLGCRQQLTGAAITGIGQAAFEE
                     IAYDNGQLINPNLIDYVLPSLADMPAVIDPIAIEIPDRNGPFGAKGIGESALIPVAPA
                     IANAIFDAVGVRIKDLPIKAEKIFLALEDLKNQP"
     gene            4949..5386
                     /locus_tag="VF182_11615"
     CDS             4949..5386
                     /locus_tag="VF182_11615"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6437771.1"
                     /translation="MTRRSGLLSLAVFAAALCACNSEILQKQAEQIKQQELEIAKQRK
                     DLEAMLAGQKLQDQKQQDCNRAFGDYFEKAQMASNGDQAIALYREGLSLCPDDEVAHY
                     ELGRALAARGQNLEAEKEFETALHLRPDFTDARKQLESVRKNR"
     gene            5531..6304
                     /locus_tag="VF182_11620"
     CDS             5531..6304
                     /locus_tag="VF182_11620"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX6437772.1"
                     /translation="MRIVLGFLLILIVFAASVHGADDNKLLDMTYPFAENTLHWPTAK
                     PFHLEKTNEGRTAAGYWYSAYDYSGSEHVGTHLDAPFHFAEGKWTTDQIPLARTIGPG
                     VIIDIRGKVDADRDYRLNLQDIQAWEKRFGRIPKGSIVLMYSGWGRYWADRKQYFGTD
                     EAGDVANLHFPGFSKEAAEFLTKQRDVKAVGVDTPSIDYGPSRDFIVHQILGAANVPI
                     FENVAALDRLPAKGATIFAIPMKIKGGSGAPLRIFAQLP"
     gene            6419..7807
                     /locus_tag="VF182_11625"
     CDS             6419..7807
                     /locus_tag="VF182_11625"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX6437773.1"
                     /translation="MTGWKSTLFPCLSVCVSLGTVYPMSVSLAADMVLQVQTTPATQS
                     PPQGKLLTIEDAVRIGLENHPRIRAARERIGSQEAILGQQMSAYYPTINFNNSYRTTN
                     SSGTSGTTSSAADTYASQANLNLTLYNFGKREGTVQAARETVDATREDFSTTTQDIVL
                     SIKQAYYIYLGTQALVNVSQETVRNRELLVRQAQGFYEVGTRARIDVARAEANLYTAR
                     ADLIASENSVKIAWVTLRNAMGSPRLPEQPVAQDSPEVELTMNLAGARTIAFDNRTEL
                     RSFEAQRRASDQLIATARRGHLPDVVFDAFYGRRNSSGGGESTFPLQPAWQAQVSLNI
                     PIFDGFRTTNRVEETLHNYYNIRAQEEERRQQIALEVEQSYLRVVETQERINATESAT
                     RAAKENLDLAQGRYQVGVGSIIEVTDAETLYTDAQTNYVRTLYDYRIADAQLARAMGD
                     NRVGVLKPGSIR"
     gene            7840..9111
                     /locus_tag="VF182_11630"
     CDS             7840..9111
                     /locus_tag="VF182_11630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX6437774.1"
                     /translation="MKRTLLLVTLLVVVGAFAGFQQYSSWSLNRSQNNPELKSAGGAA
                     QTSPNQSRAANQSQGGARSRDAVPVLIATAVRKAVPVQLHAVGNVEPYTTVSIKSQVT
                     GVLLEAHFKEGQDVKKGQQLFTIDPRPFEAALKQAEANMQRDNAQLKNLREQVRRYAE
                     LVEKQYVSREQYDLIKTNADAAEAVVEADKAAVENAKVQLSYCYIYSPVTGRVGSLLV
                     NEGNLVRVNDATPLVIINQVNPIYVTFSVPEQHLSDLKRHMNVGQLKVDASFQSDEGR
                     SEQGRLEFVDNAVDRSTGTIKLKAVFANNERRLWPGQFVNVALTLTTQPDAVVIPSEA
                     VQVGQEGQHVFVVQPDKRVEVRPVTLGTTSGGEAVILKGLAAGEQIVREGQFLLGPGS
                     RIEIKDTVVSQGKEEKRERKTKAKAQEGDPS"
     gene            9108..12311
                     /locus_tag="VF182_11635"
     CDS             9108..12311
                     /locus_tag="VF182_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX6437775.1"
                     /translation="MNISGLFIRRPVTTTLVMLGILIFGIMGYKSLPVSDLPTVDFPT
                     VTVNANLPGASPETMASSVATPLEKNFSTIAGLDSMSSTSSLGRTQITLQFSLGRNID
                     AAAQDVQSMIARSARDLPQNMPSPPSYRKVNPANNSILLLALKSPTLPLSTVDEFAQT
                     ILAQRISMISGVAQVDVIGSQKYAVRLQLDPNQLAARQIGIEDVVAAVQRGSVDLPVG
                     TLNAPNRAYTLVSDAQLTNAEAFRPMVVTYRNGAPVRVQDLGKAVDSVENTRAGSWFN
                     NERAVILGVQRQPGANVVEVVDAIKAVLPTLRAQIPQSIELELFFDRTQSIRESVHDV
                     QLTLIGTVFLVVLVIFLFLRNISATVIPSLALPMSIAGTFAIMYLLDFSMNNLSLMAL
                     TLAVGFVVDDAIVVLENIVRHMEEGERPYDAAIKGAKEIGFTIVSMTVSLVAAFIPVL
                     FMSGMLGRLLREFAVTICVAILVSGFISLTLTPMLCSRYLRPPSEQKHGWLYRFLGRC
                     LEGMNRAYEVTLQWVMRHHVITAASSLVILLATVYMFGLVPKGFIPSEDTGQVLINTE
                     GAQGVSYEQMVQYQQELAAIVAEDQNVESFFSSVGVGGSGLTGNTGRIFMKLKPRSER
                     PLSADELIRELRPKLARVPGIRVSLQNPPVIRVGGRLSRSLYQFTLQSPDATELYRHA
                     AAFEEKLRALPDMRDVGSDLQLKNPQLQVVIDRDKMATMGITAQGISPRQVEEAFWNA
                     YATRQITTINTPNNQYQVIVELDPKFQNDPSALSQLYIRSTTGQTATAQPMVPFGALA
                     TLIYSAGPLSVNHSGQLPSVTFSFNLRDGVALGDAVETINGLARENLPGTISTTFQGT
                     AQEFQSSIKSLWVLLALAVFVVYLVLGILYESFIHPVTILSGLPSAGLGALIALYLLQ
                     MDLSIYAFVGLIMLIGIVKKNAIMMIDFALERQRKDGKSPAQAIYEGALVRFRPIMMT
                     TMAAIVGAIPIAIGWGAGAEARQPLGIAVVGGLLVSQILTLYITPVMYLYLERGQDFL
                     VSLPVRLRGRDKGAQAGAATATIGAKLGVENPGQRNP"
     gene            complement(12712..14577)
                     /locus_tag="VF182_11640"
     CDS             complement(12712..14577)
                     /locus_tag="VF182_11640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX6437776.1"
                     /translation="MKNKFPLALTAVVIGISGCSSLSMPSVPWFQSAAPVDPTAEALF
                     AEGSRYFNEKKYARAIDVLQKLKSGHPFSPLLTETELKVADAYYLNQQYPEAINAFKE
                     FQSLHPTNDNIPFVILRLGQAHLDQFTSNDRDQKNTEIAKGYFETVITNHPKSPHAPL
                     AAEKLAKCVEHLSEHEFNVAVFYHQQEKFPAARDRFEEIVRKYPGTPTAIRSLYYLGE
                     SYRKEKNWVKASLAYEALIQHYPETKFAADAKTQLAQLDKEKQDPLALLLMRDRRPGA
                     APAAETKEEPALSKLKDLNLVTKKDLVHEEPGDEKGLLRRVADKLNPFSSSDEPKESA
                     TELLVKRKEADSKESSGILASLWPFGRQSSSSNNKPSQNTALVGHIDDSLKQKGIDNA
                     GQASLTPPVADLPKNDEAPAQKMDPGVLLGSIDSNLKKSGKSAELPPAPEAAAAFKDP
                     AAVQNANTTAKAAAPTQSPASSGLLSSIDQKLKSQGLEPSGFELPPSPEVSERAVKNT
                     PPVKVELEPKVSSERGPLFLAPAELPSHATSGHADDADKNNVKNDGAEKQQPAGSEAP
                     RNLVRGPTPSLPANRATKAPEQKKPAPDEEETKGLFDYLRDDAENVSKILNPFRW"
     gene            15606..17129
                     /locus_tag="VF182_11645"
     CDS             15606..17129
                     /locus_tag="VF182_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437777.1"
                     /translation="MKKRFLGLGMLSAFLFAVPVLAQNVDQKIQALEQELLQLKEQQI
                     ELKKEATEAAAAMPTFSYRPGNGLNIEAADKSWSFRPTIETYFRMLFEEGRDQVGRTN
                     GEIMGRRFRPGFFFCVNNCLWELEATFDMDGFGTGNAKNATGDDVGSILQRGALNVHL
                     ENLNPFLPTVQLGMEVSGSMSSARQGSSATGSQAEYDLHTRNNGFNTGRAGQGILLNW
                     DDRSLSGIGIPGRIGRFQLGMASFGESDDGLSSFTDKKDFNTYLSLQPFSQTKSFWIQ
                     GLTFEMGAWFCNADDRALNNGCSRYRIQDHGDGGRQTLFDSGADSIGDGLHIGLTPGI
                     IWNLGPYTLRAMAHRQRSEDRGGTPGQKKGDSWLIGHDLFLWSPKGFLTGSANTPGSI
                     LLGTHFERVDMEVGCNGGTGISCASVVAAGGSAQLNQFHRNRILLREADLWYFVAPRM
                     SIGVNWLWYDASNLRNGNNQAAHNLGVCDTPATPANGCRNGKGGDWHTLFLNWRYTF"
     gene            17310..17936
                     /locus_tag="VF182_11650"
     CDS             17310..17936
                     /locus_tag="VF182_11650"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6437778.1"
                     /translation="MNSSLIGRRSVPVFFTMILLMVSIGCNHAIPRFGVGGRYEEGRD
                     QFFRGKGGDMDRAVDALEYVVSRDPLYKDSLTYLGRAYYRKGRYEDAFAILQRAVAVN
                     KDDEVAWIALGLTQMRLGQTDKGLGSLKGGITLAHKVMVEGYHNHQNWDSRGVIRGAL
                     RRSAFLLAKGPEERDNILQTNDRLLALVDDEENFQKNARLQNSNQIYN"
     gene            complement(17940..18581)
                     /locus_tag="VF182_11655"
     CDS             complement(17940..18581)
                     /locus_tag="VF182_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437779.1"
                     /translation="MKNLSSIGLLSLILLVSGCAGFQAAREVNSGRQAYLSGNNEVAY
                     GHFQRAAEVDPNYVYGTALRQGVWSYVGRVNYDMGRLPQAREALERALAANREEDLSR
                     LYFGLALAREGDRQRGLKEIEGGLRGVHEWLEYVDQNQGASFGRFWDPAREIRSAIQT
                     DLAMISGRDIDWPKLIAAGEWVGKQMELESDRASRDETIDRNRDNDSGGGEEP"
     gene            complement(18816..19610)
                     /locus_tag="VF182_11660"
     CDS             complement(18816..19610)
                     /locus_tag="VF182_11660"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6437780.1"
                     /translation="MISLAFISTLTALGVIGGFFSGLLGFGGGVMMFPLLYYIPPLLG
                     LSRLDANTVAAVVISQVFFSTIIGGMAHLRQRRVHRPIVLGAGPSSAIGSFAGALLST
                     KVSEDLLLALFGIITVLVLAMMFLPVPSQAQEDLPREQVSVALLPLTLYSLAAGVLIG
                     LLGAGNFVFVPLLIYVLKVPTRIAIGSSLFIAMMNTSSGFLGKILTGQIPLLLTSAVV
                     MGAAIGAVLGEKLHGKISAKTLRQIYGVMVALIAARVWMTLLGFDT"
     gene            complement(19607..20497)
                     /locus_tag="VF182_11665"
     CDS             complement(19607..20497)
                     /locus_tag="VF182_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HEX6437781.1"
                     /translation="MLVEKIRNYASLVRFSHTIFALPFAFASLVLAWPNHPITLRTVF
                     WILIAMVGARTAAMGFNRLADKKFDALNPRTAGWELPNGTVKPWEAGFLTIVASILFV
                     FAAYQLNWTCFVLSPVALTVLFFYSLTKRFTWASHLFLGIALSLAPIGAWLAVTDSPL
                     GLKELLIPVCLGLAVVFWLAGFDIIYSLQDREFDRAHGLYSIPARFGVVWALRLSSFF
                     HLCTIVFLILVGITADLGSIYWIGCLAVSFILMWEHRIVRPHDLSRINRAFFDFNAYV
                     SIGYFLITLADVLVSGTSVG"
     gene            complement(20582..22024)
                     /locus_tag="VF182_11670"
     CDS             complement(20582..22024)
                     /locus_tag="VF182_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HEX6437782.1"
                     /translation="MAYNSLQDFVQVLERQGELRRINYPVKAELEITEIADRVMKNNG
                     PALLFENVPGKQIPVLINAFGSQKRMALALGVDDVEEVAGEVQKLVQTRPPRSFSDKL
                     HLLSDLLKLAGIPPKIISDGECQQIIHRQPDLSLLPVLTCWPGDAGPFITLPMVFSKD
                     PGKGTRNVGLYRMQVFDSQTTGMHWHLHKVGARHYQHQKEQSGRMDLAVCLGGDPAMI
                     YAATAPLPDQIDEILFTGFLRKKGVRLVKGVTVDVEVPANSDIVIEGYVDPAEPLRRE
                     GPFGDHTGFYSLADDYPVFHVTCITQKEHPIYPTTIVGRPPMEDAYMGKATERIFLPL
                     LRLTFPEIVDMNLPVHGVFHNLAIISIKKEYPAHARKIMHALWGMGQMMFTKILVIVD
                     HDVNVHDLSEVTWVTGNHIDPQRDTVIVEGPVDVLDHAAPVLGYGSKIGIDATRKWRS
                     EGFDREWPEVIVMDENTKNYIDSIWQKLGI"
     gene            22321..24396
                     /locus_tag="VF182_11675"
     CDS             22321..24396
                     /locus_tag="VF182_11675"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6437783.1"
                     /translation="MKKASRQQQKKAEPEKTPGTAAKSPASVDSGNTRFWFLTIFAVA
                     FAIRLVYLFQIATIPLFEHLAGDARTYYEWGQRIAAGDWLGTGVFYQAPLYPYFLGIL
                     QFFFGETLWLVRLIQITLGAVSCSLIFLIGQQLFSRQAGIISGFILASYGPALFFDGL
                     IEKSILDLLLLTVILFLVFCAGGGLTVKWLGVGAVLGLLALSRENALILVPVVPCWIA
                     LSSRSISATSRLPQIGLFFAGLTLVLLPVGLRNLALGGEFKLTTSQLGPNFYIGNNPA
                     ADGTYNSIRSIIGEPQLEGRDAKRLAERALQRNLSPGEVSNYWLGKSFEYIKKEPADW
                     LRLLAKKWTLVWNAREIEDSDDFYIYRQWSSLLSVLGRLNHFGVLAPLAAAGLLLTLG
                     QWRRLWLLYAMILALAASVAGFYVFGRYRFPLVPLLVLFAGAGLIELVRFYKQGRWRP
                     LLGVMAVFCACAIFTNWPVFGFSGPGAAGYNNLSNAYYKQGKVEQAIETALKAIEREP
                     GYGVAHYNIGNLYAGQGKIALAQRHFEEALRIYPNYAEVRSNLGQLLAEQGDLQAAIE
                     QFERAIALDSGLFRAHLNLGVALAKQGQIAKADAPLREAARLRPQSTEARFYLGSVYA
                     AQNRFDLAAESFKNALQIDPSLAAAHESLGRVLSVQGKKEEAVLHYQEAIRLMSGRKG
                     GAKGSVTIR"
     gene            complement(24433..26436)
                     /locus_tag="VF182_11680"
     CDS             complement(24433..26436)
                     /locus_tag="VF182_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437784.1"
                     /translation="MPAKHKTRKISRQDKRDSAVSATGSLANPHRRLLTGPHGRLFER
                     PVIVIVAISALHAFGLICLFDVFAGLYNPDPIIDQDWGLHFHHLKSLEAFWQDKSTWG
                     YNPLFMAGYPSNTIQDLSIKLFEFLALALSLIALTPIQWFKISAFIAMAAVPWLMYFS
                     ARNLFAREETRNAVAVLAALLGTIYWWNSLPREMFFYGMIGYSTAAYASGLGVSLFYR
                     LTRQQTSWGAVHLGWIFFAIAILPLHVQSLVIFVPPILALLIAQPKLMQRDLALWTAG
                     AAAISLSLNLVWIMPAFSHRGDDVSKTIVEQLPLFVSTDPLTLIKDYFAPKNFWSFRQ
                     SFWEKGFRLLLLILGTTGTVTLLRSRDKNVAILLGAALISLFLITYFSFFIPPLQAWQ
                     PLRFKVPFDLFLTVGAAYALADWLGAGSTKSSFYSYTVPFLLFTASITFLINLVGTES
                     QAKLRLRTLVRPEITEIVGWVRQDTPKEARVLFEESGDETGFVYDGMYLSAFIPHWSG
                     RELIGGPINLYNDRHHFAEFHSGRLFGRDIQAFSDEEIKQYFRLYNIGAVVAFHPASV
                     KRLRSIPGLITLDRRLGPVHLMRVNQPPYWFLQGHGQVVAKLNRLEVSTAAASEVILK
                     YHWLDGLISNPAATIIPVKIYDDPIPFIKVVDPPRSFILTIVP"
     gene            26707..27447
                     /locus_tag="VF182_11685"
     CDS             26707..27447
                     /locus_tag="VF182_11685"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX6437785.1"
                     /translation="MLGVALVLGFLTALANILGSALAVLQREPSRRFTAAALGLSGGF
                     ILAAALLEMLPESLSRGPSMPGYVAVGYLSLFLIEQFTNVHLHSLPDDEHRMPVPVAA
                     GFASLVAFNVHDFIDGLAIGAGMIAKTDLGILVFLSVLLHEIPAGFVIAALMRAAGWS
                     RWSAMVAGGSLGVVTILGIVLPFWARELKPFFAEALLAFAAGNFIYVGATLLVPLSES
                     GKSRWITLLVVLGFAIFYVSSRLVESFF"
     gene            27486..28022
                     /locus_tag="VF182_11690"
     CDS             27486..28022
                     /locus_tag="VF182_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX6437786.1"
                     /translation="MFLKLLCATMLVILAAACSMSPEQPSRATAELKDKDGKAVGVAT
                     FRESSGGVTVEVNVKGLTPGLHAVHVHAVGRCDAPAFTSAGGHFNPAQKKHGYKSPEG
                     AHAGDLPNMLVAKDGTGRFESFTDGMTLKPGATSVFDKDGSALVIHAGVDDNVTDPTG
                     NAGDRAACGIITMAQSRM"
     gene            complement(28028..28771)
                     /locus_tag="VF182_11695"
     CDS             complement(28028..28771)
                     /locus_tag="VF182_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX6437787.1"
                     /translation="MKAMVLAAGQGTRLRPLTDTRPKALVSVGGRPMIEYSLLLLRHY
                     GIRDIVINLHHHGDQIETRLADGSQLGLQISYSLEPELLDTGGGLLKAKPFLQSNTFI
                     VINTDVLIDLHLDQLLTFHEKQKAAATLVLRPDSDADRYGSMDIDEQGRIRRFLDTRT
                     PAQSPGPIRKLMFTGVQVLEPTVFDYMDNGMTAHKFSTTRETYPRMLLDGQPLYGFCF
                     DGFWQDLGTADRIQEAERSLAAGTRLHYL"
     gene            complement(28768..29838)
                     /locus_tag="VF182_11700"
     CDS             complement(28768..29838)
                     /locus_tag="VF182_11700"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX6437788.1"
                     /translation="MDHQKIISDKIGAVFGANTAVSSLVPLAGDASSRRYYRAVIEAS
                     GAPKSVIVMELPAGSALPLSSEELAVFKHAPKELPFLNLHRFLSGIGVRVPELYGHWQ
                     NDGILLLEDLGDTALWDRIQGLPEDEVLTWYQKAIDELLVLQIRGTKARDDACVAFQQ
                     RFDFRLYMWEFDHFIEYGLEKRPGVYVAGTGIETLRRIFSKIAHRLDRYPSCLNHRDY
                     HSWNLMIHDKEVALIDFQDALLAPPQYDLASLLNDRITDSVILPHMEDQLVSYYLRRR
                     ADIEKRAIDADDFREIYLLSAIQRDLKVVGRFYYLDLVKGKPAYRKFIPPTVRRLKRN
                     LARVPLLEPIVPLLAEHYEEMR"
     gene            complement(29886..30104)
                     /locus_tag="VF182_11705"
     CDS             complement(29886..30104)
                     /locus_tag="VF182_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258239534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(30063..30065),aa:Sec)
                     /transl_table=11
                     /product="UXX-star (seleno)protein family 1"
                     /protein_id="HEX6437789.1"
                     /translation="MSSEPVLIFGQDLUPYTSGAREDFSRRKISFEYINVLEDDDGLA
                     RMLKLSNGRRQIPVIVEAGKVTIGFGGS"
     gene            30222..31061
                     /gene="rsmI"
                     /locus_tag="VF182_11710"
     CDS             30222..31061
                     /gene="rsmI"
                     /locus_tag="VF182_11710"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX6437790.1"
                     /translation="MPGTLYIVATPIGNLEDITQRAIRTLKEADIIAAEDTRHTQILL
                     SQFTIDTPLTSYHEHNERAKAGQLVARLERGQSIALVSDAGTPAISDPGYRLIVEAIR
                     MGIRVVPIPGPSALIAALSASGLPTDGFNFRGFLPSRKRERRSRLQELRSDRYSIVVY
                     ETPHRLKESLDDIREILGDRRMVLAREVSKIHEEFLRGRVSEVIGEVRHREIRGEVTL
                     VIEGCSDLDPPSEDVLSEEIARLIADGMRVKEIAEVLGEKYGYSKRQIYALAMDQARS
                     TNS"
     gene            complement(31051..32439)
                     /locus_tag="VF182_11715"
     CDS             complement(31051..32439)
                     /locus_tag="VF182_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6437791.1"
                     /translation="MAPGSKLHAGKHGRILIVDDDRQMCQLLVDVLAEEGYDVEAAHD
                     GRSALEKFRAAGCDLMITDMMMPRMRGTELVKEVKAIDPDALILLITAFGSIESAVEA
                     MKAGAYHYVTKPFRTDEMILHVSRAFEQRRLCQELQRLRSEVLERNSFENIIGKSEKM
                     QEVFSIIRQTSDLAANVLILGESGTGKEMVARAIHHHSSRAEGPFIPLNCAAVPETLL
                     ESELFGYLRGAFTDARKDRRGLFQEASGGILFLDEISEIPINLQAKLLRVIEDKEVRP
                     LGANQSERVDTRIVSACNLDLEHLVEQGRFRQDLYYRLNVIRIELPPLRERSEDIPLL
                     AEHFLHKFAPQAKRDIASIDREALAGLVNYRWPGNVRELEHTIERAVLLGKETSIGLK
                     DLPLPSQASNGSSVPLAAAVANGYTLKDLEREYILRVIESTYGNKAEAARILGVDRTT
                     LYRKLEEYKVKS"
     gene            complement(32423..33955)
                     /locus_tag="VF182_11720"
     CDS             complement(32423..33955)
                     /locus_tag="VF182_11720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6437792.1"
                     /translation="MKLGTRLIVSLVVIIVVTMTVHGYLSIQQDRENVTREIRVGMRG
                     FSRAIHSALRDLYADGQNLQATQEFANTVGPRGNIHGLIVYNLNAEPVVLSSSLRYPN
                     DFDELDPTAILKLDPRPALAEPNGIDGYIREPGILVYYRIEPFTSSDGRTAGVFVLAR
                     QGSQLISNIEARRQRIIGTTAALVILLSVLILIIVRRNVTRPINQLVERVREIGRGRW
                     EQRIEIKGRDEIAALAGEFNSMSEELNRSYSKFLEEQQQKLNLERDLRHSERLASVGQ
                     LAAGLAHEIGTPLSIVRGRAEYLLRRPRSSEELAGNLEVIRSQSDRITAIVRQLLEFS
                     RRREPMFRSVELSELLNHVRYLLEHQLLEKKLDVEIHVSPSPLFIQADPDLLQQVFIN
                     LFANSLHALGPGGVIKIQASAMPFVGGHGSVHIIFEDNGIGIAPEAIDHVFDPFFTTK
                     DVGEGAGLGLAVTYGIIKEHNGEIIVESEPAKFTRFIIHLPLGQQKEAPVQGNLENGT
                     RV"
     gene            34327..34449
                     /locus_tag="VF182_11725"
     CDS             34327..34449
                     /locus_tag="VF182_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437793.1"
                     /translation="MKKLLFAFLLGLTVVSSIVDLATPVSAQDEDPKPRKPEGD"
     gene            complement(34525..34824)
                     /locus_tag="VF182_11730"
     CDS             complement(34525..34824)
                     /locus_tag="VF182_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX6437794.1"
                     /translation="MSTTPSQTRIRLIVKGRVQGVYFRASTVQCARHLGLTGWVMNRY
                     DGSVEMVAEGRPDRIEELIVWCRQGPPGARVDEVDLQRQDFRGEFSGFRIKRPLD"
     gene            35047..35358
                     /locus_tag="VF182_11735"
     CDS             35047..35358
                     /locus_tag="VF182_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX6437795.1"
                     /translation="MGAKLYVGNLPYSVTEERLQQHFAQQGSVISARIVTDKFTGRSK
                     GFGFVEMSSDGEAERAINALNGTDFEGRNVVVSEARPQAPRDSGFGGGGSAGRPRRDR
                     Y"
     gene            complement(35352..37373)
                     /gene="ligA"
                     /locus_tag="VF182_11740"
     CDS             complement(35352..37373)
                     /gene="ligA"
                     /locus_tag="VF182_11740"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX6437796.1"
                     /translation="MAKNLEIAAENEIIVLRQQLEKHNHQYHVLDDPLISDAEYDRLF
                     QRLIELEAAHPQFASPDSPTRKIGAPPLDKFRTVRHTLPMLSLSNANDQKDLAEFEDR
                     IVRFLRATEPIEYLVEPKIDGVAVELVYAEGRLTAGSTRGDGINGEDITLNLKTIRSI
                     PLLLRAGARPVPQRLEVRGEVFLSRAAFQRMNREREEGGQAVFANPRNAAAGSLKQLD
                     STLTAKRPLDVFFHGAGVITGASLTSHAEFRAAIQDWGLKPVPFARVCRGIEDILKFR
                     DEMESRRDELPYEIDGLVVKVNRHELQRRLGEIARSPRWAIAYKFKPRQATTRIIEIQ
                     PQVGRTGTLTPVASLEPVAIGGVMVKSASLHNMDEINRKDIRVGDTVVVERAGDVIPY
                     VVKVITEKRTGQEKHFCMPEHCPVCGAAVYREEGEAAYRCIGISCPAQLKESLKFFGS
                     RNAMDIEGLGDKLIDQLVERGAVKDLGDLYSLTEERLASLDRMAKKSAQNLLAALQRS
                     KDSTLTRFLTALGIRHVGEATAKLLTAHFGDLNTIMNASEEKLMEVREIGPEVAKSIA
                     RFFSQDENRQVIAKLLHSGISFKAETIKQGALSGSTFVLTGGLETMSRVEAQRRIEAL
                     GGRVSSAVSRNSSYVVAGSEPGSKLKKARELGVRIIEEQELLKLLTPDQ"
     gene            37498..39243
                     /locus_tag="VF182_11745"
     CDS             37498..39243
                     /locus_tag="VF182_11745"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6437797.1"
                     /translation="MRTRFYGSAIWLLILSGCASSGAVLKAPANGLFGSPEAPFLQQQ
                     RVEVPAEARGLSHFMKGHLLLGEGRFDEALEEFESASQANPQDAFLRFRLATLYVRKG
                     DLKRALVEAEAAKRMDPNSVENRLLLAGLYSSLGENQKGIAEYQEVLKLDPKNQEALL
                     YLGALYLQNDDNSRATEMLDTLLKLDNDSVLGHYYLGRVRAKSKLYSAAEQSYRRALE
                     LNPKSEAVLMDLALVYELQSKSEDAIQTYQRLLGLNAQNVWARKRLGELYVGQNKLDD
                     ALAQFENLKSSEADPRETRIKIGLIFYEKGDFERAATEFNLVLAGDPENDRARYYLGA
                     TYVELKSDEKALGEFAKIPEKSDLYLDSRLQMAYLYERTDQLQKAIDSVQSVLRKKND
                     RKEVYAFLSTLYRKAKEFPKAIEAMERVIVLDPKSDQAYFQLGALHDEAKNKEESVVN
                     MKRAIELNPKNAAALNYLGYTWAEMGVRLDEAEQLIQRALQLEPNDGFYIDSLGWVYF
                     QKGDYARAVEQLERAVAITVDDPTIIEHLGDAYLKFGNFDLALSRYREAIQKSKEDDQ
                     VKRIRDKIEHLEKKI"
     gene            39240..40061
                     /locus_tag="VF182_11750"
     CDS             39240..40061
                     /locus_tag="VF182_11750"
                     /inference="COORDINATES: protein motif:HMM:NF025490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HEX6437798.1"
                     /translation="MRAALRRELSVRVQNFVFLFAAAVFTINGCATVKPPPAKPELPA
                     KQWQTGELIHSLGERQKQFRSLRALARLDYAGPDGKGNVQEAVLVQRPDRLRLETLTF
                     LGAVLIVTVNDREIIGYHPREGIFVRGPRNKENLFRYTQIPLELDEMTALLLGLPPVD
                     TGAPVRQEGPALVFSPNGLKQDAVAFEAQAPLPTKWERFNDAGAVELSAQFMDYVLTP
                     AGPFPSRIQIEAELQKRKLDIRYQQPEVNASLAPEIFTQEKPAHAEELRLETLGH"
     gene            40064..>41253
                     /locus_tag="VF182_11755"
     CDS             40064..>41253
                     /locus_tag="VF182_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437799.1"
                     /translation="MFVARGVGVLLLCLLLAGCRDQAEITVGDSQNIVDDKNAGAPTT
                     GDWLIIHALSDPEQLNPLTSNDAGSSEINGYIFESLLSRDPRTLELKPIIAEARPEIS
                     QDKLSYTFKIRKNARFQDGRPVTGEDVLFSVKAIKCPLVNAPFLRVYFNSVVDAQLLD
                     PYTIRFFIREPYFLNESVLGGVIVMPRHYYDRENLLRSITVRQLAQDPTKLPAEVKKF
                     ADVFNRNYNRNPLGSGPYKFSHWKTGSETELTRDTDYWGNGQEGIDQPHVDKLKFRVV
                     NNMDAALTRLKSGSLDYMESLQPVQVVRGTSSERFKREFKKYEYYAPTYTYIGWNNDH
                     PIFGDKRVRQAMTYLTNRKQIVDTVLFGLGEVVDSPIYFFRPEYDKTLVSYPFDPAKA
                     LALL"
BASE COUNT         9546 a        10963 c        11374 g         9370 t
ORIGIN      
        1 aggcttggaa gtggccaccg ccgttggcgt cgacccagcc atcgtcgtcg gctcccaggc
       61 gcgggtgccg ttcggcatcg atgagtttca tgtcgccggc ggtttgcgcg gcgccgcggt
      121 aaaattaacc aggtgcgaga ccattgatgt cgaggtaccg gcattcgccg aagtcgtgat
      181 cgaaggtgtg accgttccgg gcgaaagggt tgccgacgga ccgtacggtg aatatcccgg
      241 ctgctacagc gaagccaagc aagcccccgt gctcaaagtg acggcgatta ccatgcgcca
      301 taatccaatc tggcagacgg ctctcaccgg catgccggtg acggaaaatc acactttgat
      361 cgaatatggc aatgccgcgg tcgtttatcg agaagtaaga aaggttgtgc ccgaagtccg
      421 cggcattaac atgacgccgg gaggcacctt tcgccaccat gtggtggtgt cgatcaaaaa
      481 gcgcgccgag aacgaagggc gcaacgttat tctggcgctt ctttcaatgg gcatcggcct
      541 caagcaggtg atcgttgtcg atgacgacat cgatcctttc gatcccatgc aggttgattg
      601 ggcgatggca acacggttcc aagcggacaa agacgcaatc gtcatcccac gcatcgcttg
      661 ctcgacgctt gacccgtcgt gtccggaacc ccgggtcacc gccggcctcg gcatcgatgc
      721 caccgcgccg atgaaagatc ggtggcgctt tgaaaaagtc gaaatccccg gcgtcgataa
      781 agtgagttac atttaaaaga ggataagctc gagatccgaa atcgcaaatc cgagaaaatt
      841 aatgatcaaa acggaaagca cgaaaccgaa gcaggaagct taaagcaaat ccaaatgacc
      901 aaaacctaat gtgacaaaca ttcgtgattc ggattcaggg ttttggattt tgttggtttg
      961 aaatttcttt ttagctaaat ttgttgcgga ttttgctatt cggatttcgg attttcatga
     1021 accaagcatg agaaacttcg actatctcga gccgacgacc gtagcggagg cgtgcgccat
     1081 gctcaagcag catggcggcg aagccaaagt ttttgccggc ggcgcgcatg tcacgatact
     1141 tatgaagcaa gggctctatc agcccaaggc gttggtcaac atcaagaaga tttctggtct
     1201 aaagaacatt tccttcgaca ccacggaagg attgaccctc ggcgcgctgg tgacgcaccg
     1261 tgaaattgag acctccgcac ttgtgaaaga aaagcttcca gtgctctcgg aggcggaaag
     1321 agaaatcgca aatatccgcg tgcgcaatat gggaaccgtc ggcggcaatc tcgcttccgg
     1381 cgaaccgctg accgatctgt cgcaaatttt tattgcgctc gacggcacgt taagaatcgc
     1441 cgggccagac gggcaacgcg ccatccgggt cgaggagttg tttcttgatt tttacaccac
     1501 cagtttggcg gaagacgaaa tcatcacaca agtggtgctc ccgccgctct cgccgcattc
     1561 cggcatcgac tacatccgat tttccagcag ctctgtggtc gacaaacctt cagccggtgt
     1621 cgcagtgcga ctgacgttgg acaaagaggc tgttcaagtc gccaggatcg tgctcggctg
     1681 cgtcggtccg acaccggtca gggcgaacaa ggccgaggcg cttctgatcg gcaagatact
     1741 gaacgctgag cttacgagag aggccggcgc gctggcatcc caagagtgca gtcccatcgg
     1801 cgacctgcgt ggttccgaag aatacaaacg agcgatcgtc aatgcgctcg tgaggcgggc
     1861 gacggatgcg gcgtacagaa aatctctcaa ccaataatta cgaggaaggt cggagcattc
     1921 atacaggatg aatttgtcag agttcgaatt gtccctttcc cctccatggg aagggttagg
     1981 ataggggtga gttccgagcg cataagcctg ttcctatggc caggccttta cagagtagaa
     2041 ctgtaatgaa tcgcaagacg atctcaatga atgttaacgg ggtcgaacgc aaggtcgaag
     2101 ttgaagcgcg cactactctg ctcgatttgg tgcgcgagga attcggattg accggcgcca
     2161 agctcggctg cgatatccag gtatgcggcg cttgcacgtt attgctcgat ggcaaaccgg
     2221 tcagcgcgtg ctcggttttg gcggtcgacg cggaggggca taacgtggtg accatcgagg
     2281 gactgggcag cgatagtaag ctcgatccaa tccaagaagc ttttatggaa tttggcgctt
     2341 tgcagtgcgg ctactgtacc tcaggattca tcttgactgc gaaagccttg ttggatgaat
     2401 gccctaagcc atccgatgag caaattcgaa attacctagc cggcaacttt tgccgctgcg
     2461 gctgttacca ggagattatc caagcggtca ggaaggtatc cggagcggct gattaatacg
     2521 accttggcgg cccctttttc atcttcgccc gcgtgcgggg gaaagaggtg ggggaaaatg
     2581 cgaggactat gtgggatctt caatgagcac aaacgaaatc ggcaaatccg cgcgacggtt
     2641 ggattacgag agcaagattt caggccgggc gatgtatctg gcggacatgc acatgccggg
     2701 catgtgccac ggcaaaattc tgcggagtcc gctgccccac gcccgcatca aaggcatcga
     2761 tatttcacga gctctcaaag ttgccggtgt tgtcacgatc atcacccgcg acgatattct
     2821 tcatgaccaa ggtatcgaac cttactacgg tccggtgttc aaagatcaaa ccatcttggc
     2881 cgtggataag gtgcgccatg tcggtgatcc ggtagcggcg gttgcggcgg caaccctgga
     2941 tgctgccgaa gaagcgctgc ggctgattga agtcgattac gaagaactgc cgccggtgct
     3001 cgatgtgcat gaagcgttaa ataacggcgc tgtcttagtg cacgattcaa tcaagctacc
     3061 aagttccggt ttcgccgacc ttgccgaaat caagcccgtg gaagggacta acatctgcac
     3121 gcacttcaag ctcaaccggg gcgaggtgga gaaaggtttc gccgaagcgg accgcatttt
     3181 tgaagacacc ttcaccttac cggcgacgca acacagtttt ctggagaccc atgcctgtat
     3241 cgcgttagcg gaggcgggac gcatcactgt ctgggcgacg acccagaacc cgttcgtcgt
     3301 acgcactcag ttggccaaca tattcaaagt tccggtatcc aaggtgcggg tcatagtgcc
     3361 gtatctgggc ggcggctacg gcggtaaagt ttatcccaaa gttgagccga tcacggtggc
     3421 tcttgcgcaa aaggcgcgcc gcccggttcg cgtggtccta tcgcgcgagg aagtcttcta
     3481 cacgatcacc aagcacgccg cggtgattcg gatgaaaacc ggcgtgaaaa atgacggcac
     3541 cctgatcgcc cgcgaatgtg aaatccatct tgacaccggc gcctatgccg aaattggacc
     3601 gcgggtggcg aagaagtcgg gctacacagc ggccggacca taccgaattc ccaatctgaa
     3661 aatcgactcg tactcggttt acaccaataa accaccagcc ggcgctttcc gcggcttcgg
     3721 ggtttctcaa tcagcctggg cggtcgaatc gcagatggat atcatagcct cggccttgca
     3781 aatggatccg ctcgagcttc ggctcaagaa cggctacgtc aatggcgatc gattcgtgac
     3841 cgaggaaacc ctgcgcagcg ttggcttgaa agattgtcta gagggagtcg cgaaatcgat
     3901 tggatgggaa gataaaacgt tcgagggttc aaacgttcaa aagtttaatg acggaaatct
     3961 gaagcgcggc aaggggttgg cctgtatgat caaggcaacc atcacacctt cgatatcgtg
     4021 cgccgcggtc aagctcaacg aagacagtag cctgtcggtt tatagcggca cggtcgaaat
     4081 gggccaggga tccgagacag tgctggcgca aatcgccgga caagaactgg gaattccgct
     4141 gcaacggatt caagttctag gcgtcgatac cgatgtcgtc ccgtatgatt tgactacctc
     4201 gtcaagccgg tccacctttc acatgggtaa ggcggtgcaa ctcgcggccc gcgatatcat
     4261 gcggcagctt aaggacatcg ttgtccaaga gtatggcgtg ccggcggacc aggtgagctt
     4321 cgccgatggc aaggtgcgct tacccgaagg cgcgctcgat tatgctgaaa tcatgttcaa
     4381 aagattcggc atgcaaggcg gcactttaat gggcgagggc gaggtaaaga cctcggtgaa
     4441 agatgaatac ggcgaaaaga gcacttcggc attttggttc ttggctgcgg gcgctgcgga
     4501 agtcgaagtg gacgtggaaa ccggcaaatt caagctgctc aagtacgcca ctgcggtgga
     4561 cgtcggtaaa gcgctcaacc ctctcggctg taggcagcag ttgactggcg cggcaattac
     4621 cggcattggc caggcggctt tcgaagagat cgcctatgac aatggccagc tgatcaatcc
     4681 caaccttatc gactatgtct tgccctctct ggctgatatg cccgcggtaa tcgatcccat
     4741 agccatcgag attccggaca ggaacggtcc attcggcgcc aaaggcatcg gcgagagcgc
     4801 cttgatcccg gtggcgcccg ctatcgctaa tgcgattttc gatgctgtgg gcgtgcgcat
     4861 caaggattta ccgatcaaag ccgaaaaaat ttttctggcg ttagaggatt tgaaaaacca
     4921 gccctagcgc aggctgccga ttcagacgat gactcgcaga tccggcttac tttcattggc
     4981 tgtttttgcg gcagctctat gtgcctgcaa ttctgaaatt ctgcaaaaac aagccgaaca
     5041 gatcaagcag caagagttgg aaatcgcaaa gcagcgcaag gatctcgagg cgatgctggc
     5101 gggtcaaaag ctgcaagatc agaagcaaca ggattgtaac cgcgcttttg gcgattattt
     5161 cgagaaggcc cagatggcaa gcaatggcga tcaggccatt gcgctttatc gcgaagggtt
     5221 gtctctttgt cccgatgatg aggttgcgca ttatgagctg ggcagggcgc tggcggcacg
     5281 cggacaaaac cttgaagctg aaaaggagtt cgagaccgcg ctccatctga ggcccgactt
     5341 caccgatgcg agaaaacaac tcgagtctgt acgtaagaac cgctaatcca ggagctcaag
     5401 ataagcgagc gggcagcagt ttactctcga attctttccc ctgccgttgc aaaccaggcg
     5461 cgtttctcgg gttcttcttt tcgcggcgca gatcattctt tggatacgtt tacttccagg
     5521 aggtagatcg atgcgaatcg tcctcggatt tttgcttatc ttgattgtat tcgcggcttc
     5581 ggtgcacgga gccgatgaca ataagctcct ggatatgact tacccattcg ccgaaaatac
     5641 gttgcactgg ccgaccgcca agcctttcca tctcgagaag accaacgaag gacgaacggc
     5701 agcgggttat tggtattccg cttatgacta tagtggctcc gagcacgtcg gtacccatct
     5761 tgatgcgccg tttcactttg ccgaaggcaa atggacgacc gatcagattc cattggccag
     5821 gacaatcggt cccggtgtca tcatcgacat tcgcggcaag gtcgacgccg atcgcgatta
     5881 tcgattaaac ctccaagaca ttcaggcatg ggagaagcgt ttcgggcgca ttcctaaagg
     5941 atcaattgtc ttgatgtact cgggatgggg cagatactgg gccgatcgca agcagtactt
     6001 cggcacagat gaagcaggcg acgtcgccaa tcttcatttt ccaggattct cgaaggaggc
     6061 ggcggaattt ttaacaaagc agcgcgacgt caaagccgtc ggcgtcgata cgccgagcat
     6121 cgactatggg ccatcgcgcg atttcatcgt tcatcaaata ctcggcgccg ccaatgtgcc
     6181 gatcttcgaa aacgtcgcgg ctctggatcg cctgcccgcc aagggcgcaa cgatctttgc
     6241 gattcctatg aaaatcaaag gcggcagcgg cgctccactt cgaatatttg cccagctccc
     6301 gtaggtttat cgcaaatttt ccagcctcga aaacgtcacg attgtgcgct ccgcagtttt
     6361 tgctttcacc tgctaaaacc actatagtct gcgcgcacga agttaaggga ggaactgtat
     6421 gaccgggtgg aaatcgactc tgttcccatg tctttcggtg tgtgtttcgc tgggaacggt
     6481 ttatccaatg tcggtttcgc tggcggccga catggtgttg caggtccaaa cgactccggc
     6541 cacgcaatct ccgccccaag gcaagcttct gaccatcgaa gatgcagtcc gcatcggtct
     6601 ggaaaaccac cccaggatca gggcggcccg cgaacgaatt ggctcacaag aagcgattct
     6661 gggtcaacag atgtccgcct attatccgac gatcaatttc aacaattcct accgcacgac
     6721 taattcctca ggaaccagcg ggacgacctc aagcgcggcc gatacgtacg ccagtcaggc
     6781 caatttgaat ctgacgttgt ataacttcgg taaacgcgaa ggcacagtgc aggccgcgcg
     6841 ggaaacggtc gatgccactc gtgaggattt ttcgacaacg acacaggata tcgttctgag
     6901 catcaagcag gcctattaca tttatttggg gacacaagcg ttggtgaacg taagtcagga
     6961 gacggtgcgt aaccgcgagc tgttggtccg tcaggctcaa ggattttacg aggtcggcac
     7021 ccgtgcgcga atcgatgtcg ctcgcgcgga agctaacctt tataccgcgc gtgccgatct
     7081 catcgcttct gagaactcgg ttaaaattgc ctgggttaca ttaagaaatg ccatggggtc
     7141 gccgcgcttg cccgagcaac cggtcgctca agactcgcct gaggtggagc taacgatgaa
     7201 cttggcaggc gcgcggacca ttgctttcga taaccgcacg gaactgcgta gctttgaagc
     7261 acaaaggcgg gcttcggatc agttgattgc gaccgcgcga agaggccatt tgcccgacgt
     7321 cgttttcgac gcattctatg gccggagaaa ttcgagtggc ggtggcgaga gcacctttcc
     7381 ccttcaaccc gcctggcagg cgcaggtgag tctcaacatt cccatcttcg acggtttccg
     7441 cacaaccaac cgggtcgagg agactttgca taattactac aatatcagag ctcaggagga
     7501 ggagcggcga cagcagattg ccttggaagt tgagcagagc tacctcaggg tggtagaaac
     7561 tcaggagcgg attaacgcca ccgagtccgc tacacgagca gccaaggaaa atcttgattt
     7621 agcccaagga cgctaccagg tcggggtagg ttccattatt gaagtaaccg atgctgagac
     7681 tctgtacacc gatgctcaaa cgaattacgt gcgcacgctt tatgattaca gaattgccga
     7741 cgctcaattg gcgcgggcta tgggcgataa ccgcgttgga gttcttaagc ccgggtctat
     7801 aaggtagttt atacagggtc gcctaggagg ctcgaggtga tgaagcgtac tctcttactt
     7861 gtaacgctgc tcgtggtagt aggcgctttt gccggcttcc agcaatactc ttcatggtcg
     7921 ttgaatcgat cacagaacaa tcctgagctc aaatcggcgg gaggcgcagc gcaaacttcg
     7981 cccaaccaga gtcgcgccgc taatcagagt caaggcggcg cccgatctcg cgacgcggtg
     8041 ccggtgctca ttgccacagc ggttcggaag gcagttcccg tacaactcca cgcggtcggc
     8101 aatgtcgagc cctatacgac ggtgtcaatc aagagtcagg tcaccggcgt tctcttggaa
     8161 gctcatttca aggaagggca agacgtaaaa aagggacagc agctctttac tatcgatccg
     8221 cgccctttcg aagccgctct caagcaggcc gaagccaata tgcagcgcga caatgcgcag
     8281 cttaagaacc tgcgtgaaca agtgcggcgc tatgccgagc tggtggaaaa acaatacgtc
     8341 tccagagagc aatatgatct gatcaaaacc aacgcggacg ctgctgaggc ggtcgtagaa
     8401 gccgacaagg cggcggtaga aaatgccaag gttcagctaa gctactgtta catctattcg
     8461 ccggtcactg gccgggtcgg cagcttgctg gtgaacgaag gtaatttggt tcgggtcaac
     8521 gatgcgacgc ctctggtgat catcaatcag gtgaatccga tctatgtgac attctcggtt
     8581 cccgaacaac atctgagcga tctcaaacgt cacatgaacg taggacagct gaaggtggat
     8641 gcctcgtttc aatccgatga aggacgttct gaacagggcc ggctcgaatt cgtcgacaac
     8701 gccgtcgacc gctcaaccgg aactatcaaa ctgaaagcgg tgtttgctaa taacgagcga
     8761 cgtttatggc cagggcagtt cgtcaacgtc gctctgaccc tcaccaccca gcccgacgcc
     8821 gtagtgattc cttccgaagc ggttcaggtg gggcaggaag gtcaacatgt attcgtcgtc
     8881 caaccggaca agcgagtgga agtgagacca gtaaccttgg gaaccaccag cggcggcgag
     8941 gcggttatcc taaaaggact tgcggccgga gaacagattg tccgggaggg gcagttccta
     9001 ttgggacccg gctcgcgcat cgagatcaaa gacactgtcg tcagccaagg caaagaggaa
     9061 aaaagggaac gcaaaactaa ggcaaaggca caagaagggg acccgtcatg aacatctcgg
     9121 ggcttttcat ccgcaggccg gtgacgacga ccctggtcat gttagggatc ttgatctttg
     9181 gcatcatggg ctacaagtcg ctcccagtca gcgacctgcc gacagtcgat tttccaaccg
     9241 ttacggttaa tgccaatctg ccgggtgcca gtcccgagac gatggcgtcc tccgtcgcca
     9301 cgccgctgga gaaaaacttt tctaccatag ccggcctcga ttcaatgagc tcgacaagtt
     9361 ccttgggtcg gactcaaatc acgctgcagt ttagcttggg ccgtaacatc gatgccgcgg
     9421 cgcaagacgt gcaatcgatg atcgcgcgtt cggcgcggga cttgccgcag aatatgccca
     9481 gcccgccgtc gtatcgaaag gtcaatccgg ccaacaactc cattcttctc ctggctttaa
     9541 agtcgcctac gctgcctctg tcgacggtgg atgaatttgc ccaaaccatc ttggcgcaga
     9601 gaatctcgat gattagtggt gtggctcaag tcgatgtcat cggttcgcag aaatacgccg
     9661 tcagattgca gctagacccc aatcagctgg cggcgcggca gatcggcatc gaggatgtgg
     9721 tggcggcagt tcagcgcggt agtgtggatc taccggtggg aactttaaat gccccgaatc
     9781 gcgcttacac cttggtctct gacgcgcaac taaccaatgc ggaagctttt cgcccgatgg
     9841 tggtgaccta tcgcaacggt gccccggtgc gggttcagga tctcggcaag gccgtcgaca
     9901 gcgttgagaa caccagagct gggagctggt tcaacaatga aagggcggtt atcctgggcg
     9961 ttcagcgaca gcctggggcg aatgtcgttg aagtagtcga tgccatcaaa gcggtgcttc
    10021 ccacgcttcg cgcacagatt ccacagtcca ttgagctcga gctatttttc gaccgcacgc
    10081 agtccatccg agagtccgtc cacgatgtcc agctcaccct cataggaact gtttttctgg
    10141 tggtgctggt gatcttcctg ttcctgcgaa atatctcagc caccgtcatt cccagcctgg
    10201 cattgccgat gtctatcgcc ggtacgttcg caatcatgta cctgctcgat ttcagcatga
    10261 acaacctatc cttgatggcg cttaccctgg ccgtcggttt cgtggtcgat gacgcgatcg
    10321 tcgtgctgga aaatatcgtc cgccacatgg aagagggtga gcggccctac gatgcggcga
    10381 tcaaaggagc aaaggaaata ggcttcacaa tcgtttccat gaccgtttcg ctggtggccg
    10441 cctttattcc agtgctgttt atgagtggta tgcttggcag actcttgcgc gagttcgcgg
    10501 tcacgatctg cgtagctatt ttagtttcgg gctttatttc tttaaccctg acgccgatgc
    10561 tgtgtagccg ttatctgcgg cctccttcgg agcagaagca tggatggctt taccgctttc
    10621 tcgggcgctg cttggaggga atgaaccgag cctatgaagt cactctgcag tgggtgatga
    10681 ggcatcatgt gatcaccgcg gcatcatcgt tggtgatcct gctagcgacg gtttacatgt
    10741 tcggtctggt tcccaagggt ttcatcccaa gtgaagatac ggggcaggtg cttatcaata
    10801 ccgaaggagc ccaaggcgtt tcgtatgaac aaatggtgca gtatcagcag gaattagccg
    10861 ccattgtcgc cgaggatcaa aatgtcgagt cgtttttttc cagcgttggc gttggcggtt
    10921 cgggtctgac gggcaatacc ggccggatat ttatgaagct caagccgcgc tcggaacggc
    10981 cgctttctgc tgatgagttg attcgtgagt tgcggccaaa gttggcgcga gtccccggta
    11041 ttcgtgtatc gttgcagaac ccgccggtga ttagagtcgg cggccgtcta agccgaagcc
    11101 tatatcaatt cacgctgcaa agtcccgacg ccactgaact ctaccggcat gcagccgcgt
    11161 ttgaagaaaa gctcagagca ctgcccgata tgcgcgatgt gggcagcgat ttgcagctca
    11221 aaaacccgca gttgcaggtt gtgattgacc gcgacaagat ggctaccatg gggattacgg
    11281 cgcaaggcat ttctcctcga caagtcgaag aggctttttg gaacgcctat gccacgagac
    11341 agattacgac tatcaacacg ccaaataatc aatatcaggt gatcgtcgaa ctggatccca
    11401 aatttcaaaa cgatccgtca gctctgtcgc agctctatat tcgatcgacc accggtcaaa
    11461 cggctacagc ccaaccgatg gtaccctttg gagcgttggc aactcttatt tattccgctg
    11521 ggccgctatc ggtaaatcat tccggacagc tgccgtcggt gacattttcc ttcaacttaa
    11581 gagatggagt tgctttgggc gatgcggtgg agactatcaa tgggctggcc cgggagaatt
    11641 tacctggaac catcagcacg acctttcaag gcactgccca ggagtttcaa tcatcaatca
    11701 agagtctctg ggtcctcttg gcgctggccg tgttcgtggt ctacttggtt ttgggaattc
    11761 tctacgagag ctttattcat ccggtgacta ttctttccgg tcttccttcc gccggtcttg
    11821 gtgcattgat cgcgctctac ctgctacaga tggatctgag catttatgct ttcgttggtt
    11881 tgatcatgtt gatcggtatc gtaaagaaaa atgccatcat gatgatcgac tttgctcttg
    11941 agcgacaacg caaggacggc aaaagcccgg cccaagccat ctacgaaggg gcgttagtgc
    12001 gctttcggcc gatcatgatg acaaccatgg ccgcaattgt tggagcgata cccatcgcga
    12061 taggctgggg ggcgggcgct gaggccagac agcctttggg catcgctgta gttggcggtc
    12121 tgctcgtttc gcagatcctg acgctctaca taactccagt gatgtatctt tacctcgaac
    12181 gaggccaaga ttttctggtt agccttcctg tgagactgcg cggacgtgat aaaggtgctc
    12241 aagccggtgc cgcaaccgcg accatcggcg ctaaactcgg agtggaaaat ccagggcagc
    12301 gtaatccgtg actatttgtt gcaatctcgc gatcgtttaa gctcttcaga caagcgcgac
    12361 cgtcgtaaaa gggggttcat tcggtaaatg cctcgaaact ggttgtcgaa cgcacgaggt
    12421 aatcgccgat atctgcggta aatgacgcaa tgggcttcgt tccactgcgt cggttttgcc
    12481 gtacttcaac tgatcgcctg ccgccgactt ccctcgtgca tgggatcaaa tcactgcaat
    12541 tgcgcgacga gtcacaagcc tcgagcaggt ttgtttgggt aaggccggaa cttcggtcgg
    12601 aaaagaacgt cgtaactaag ccgcgttttt accggatact cgtataaatt ttcgtaccca
    12661 ccgggtcgtt acggcgaaag gtgtgagcgc ttgacttcgg tctagactcg cctaccagcg
    12721 aaatgggttc aatattttac tgacgttttc agcatcatcc cgcaggtaat caaacagccc
    12781 tttggtttct tcttcgtctg gcgctggctt cttctgttca ggagcctttg ttgctcgatt
    12841 ggcgggtaaa gacggagtgg gtccacggac tagattacgt ggagcttcgc tgccagcagg
    12901 ctgttgtttc tcagcgccgt cgtttttgac attattttta tccgcatcat ccgcatggcc
    12961 gctggtggcg tggctcggaa gctcagccgg cgccaggaac aatggccctc tctcggaact
    13021 tacttttggc tccagttcga ccttcactgg aggagtgttc ttaacggcgc gctcgctgac
    13081 ctcaggggat gggggcaatt cgaatccgga aggttccaat ccctgggact ttagcttttg
    13141 atcgatacta ctcagtagtc cgctgctggc agggctttgt gttggcgcgg ctgctttggc
    13201 tgtggtgtta gcgttttgaa cggcagccgg atctttgaag gccgccgccg cctccggcgc
    13261 cggcggcagt tcggcgctct taccgctctt ttttaaattc gaatcgattg aacccagaag
    13321 cacacctgga tccatcttct gagcaggcgc ctcgtcgttc ttcggtaaat ctgctaccgg
    13381 aggagttagg ctggcctgcc cggcgttatc aatacccttt tgctttaaag agtcgtcgat
    13441 gtgaccgaca agcgcggtgt tctggctggg cttattgttc gagcttgagc tttgcctgcc
    13501 gaatggccag agagatgcga gaataccgct ggactccttg ctgtcagctt ctttgcgttt
    13561 gaccagcagt tccgtcgcgc tttcttttgg ctcatcggat gaagagaatg gatttagctt
    13621 gtccgccaca cggcgcagaa gtcctttttc gtctcccggt tcttcatgca ccaggtcctt
    13681 ttttgttacc agattcagat ccttcagttt gctgagcgct ggctcttctt tcgtctccgc
    13741 cgcgggagca gcgcccggcc gacgatcgcg catcagcagc aatgccaatg gatcctgttt
    13801 ctctttgtcg agttgcgcca attgagtttt cgcgtccgcc gcgaatttag tttccggata
    13861 atgctgaatc agcgcttcgt aagcgagaga agcttttacc caattctttt cttttctgta
    13921 actttctccc aggtagtaaa gacttctaat cgcagtcggc gttcccgggt atttgcgaac
    13981 aatttcttcg aaacgatctc ttgccgcggg aaatttttct tgctgatgat agaacacggc
    14041 aacgttaaat tcgtgctcgg acagatgctc aacgcatttg gccagctttt ctgcggctag
    14101 tggggcgtgc ggcgactttg gatggttggt aataacagtt tcgaaatagc ccttggcgat
    14161 ttcggtattt ttctgatctc gatcattcga cgtgaactga tccaagtgcg cttgtccaag
    14221 tctcaaaatg acaaagggaa tattgtcgtt ggtcgggtgc aggctctgaa actctttgaa
    14281 tgcgttgatg gcctcggggt actgctggtt taaatagtag gcatccgcaa ccttcaactc
    14341 ggtctcagtg agcagcgggc taaacgggtg tcccgatttg agcttttgca aaacgtcgat
    14401 ggcgcgcgcg tatttcttct cattgaaata gcggctgcct tcggcaaata aagcctcggc
    14461 ggtcggatct actggagctg cggattggaa ccaaggcacg gacggcatcg aaagtgagga
    14521 gcaacctgag atcccaatga caaccgcagt gagagcgagc ggaaacttat ttttcatttt
    14581 tcacctactg atggtagttt ttccataata tgcccacagg agacagagtc aagcttattg
    14641 tgcttgtgtg ttgcgttcca ggcgatctgg tgttcgctgc tcacgaccag tgaattggcg
    14701 cgagctaaga aacggtgaat cgagatagat cgtttaaatt gttttctaga cgaagagccg
    14761 ctcgcgaatt ttgaaatcac gagctactca acgcagagtg attcagcgag ccgagatgcg
    14821 tatgaaataa aagtggcgcc gatttttgcc gagcgattta gccgcgccac ccagagcaat
    14881 gctcgcggag caaactagct ctaccgacaa ttcactctaa gatcagggag cttgtaagtt
    14941 gaccgtaaaa acccgagcgc ggaaccccga gcgcggaacc ctttgatgag tcgcccacct
    15001 caaatggttg aacgaacgcc ggagcggatt caaatctcac ctgttccggc gacactgatg
    15061 ctccgcgcgc aattctcccg ctgatccacc gaaggtttag agctgccaaa ggctcctcta
    15121 aaatctagag cttagccttg aatagctctc gctccttgaa gtcaacctaa aggcgtagtg
    15181 aattgcagcc ttgtccttga gaaaggctga gcttgaacag agcgcagaga cttgccgcgc
    15241 gacccatgag ctcgccctcg aagtggctaa aggagaaaca gccgttttta ccgggcgttg
    15301 aaaaatttgg ttggttgacg attaagctgc taacgatttc agcgcgtttc gacatctctt
    15361 ctcgagaatt acagcaaaac cccggcagaa atatatgcgc cggatctttt actaagccct
    15421 aagtcgtaga aagaagaagc cagagcgggt gtttagtggc aaaatcatac tactcgcgta
    15481 ttgaaggcac cgggccaatt caagtacaac cttgcgagac tgatggcggg gaggcgttga
    15541 tggtaactgc ttcttggcca aaaaacattt gcagcaaaaa gatacaaggg aaagaaggag
    15601 gcaaaatgaa gaagaggttt ctgggattag gaatgctatc cgcgttcctt tttgctgtcc
    15661 cggtccttgc gcaaaacgtc gatcaaaaga tccaggcgct tgagcaagag ctgttgcagt
    15721 taaaggaaca gcagatcgag ctgaaaaaag aggccaccga agcggcagcc gctatgccga
    15781 cttttagtta ccgtccgggt aacggcctta atatcgaggc agcggacaag tcatggtctt
    15841 tccgccccac catcgaaact tatttccgca tgctcttcga agaagggcgg gatcaagtag
    15901 gccgcaccaa cggcgagatc atgggccgtc gtttccggcc cgggttcttt ttctgcgtca
    15961 acaactgctt gtgggagctc gaggcgactt tcgacatgga cggtttcggc accggcaacg
    16021 ccaagaacgc gaccggagac gatgtcggct ccatattgca gcgcggcgca ctcaacgtcc
    16081 atttggaaaa cctcaatcca tttttgccga cggtgcaact cggcatggaa gtctcgggta
    16141 gcatgagcag cgcgcgccag ggttcgagcg cgaccggctc gcaggccgaa tatgacttgc
    16201 atacccgcaa taatggcttc aacacgggtc gcgcgggcca gggcatcctg ctgaactggg
    16261 acgaccgctc gctctccggc atcggcattc cgggtcgaat cggccgtttc caactgggga
    16321 tggcttcatt cggcgagagt gatgacgggc tcagcagttt tactgacaaa aaagatttca
    16381 acacttacct gagccttcag cccttttcgc aaacgaagag tttctggata cagggtctaa
    16441 ccttcgaaat gggcgcatgg ttctgtaatg ccgacgaccg cgcgcttaac aacggttgct
    16501 ctcgttaccg cattcaggat cacggcgacg gcggacgcca aaccctattc gatagcggcg
    16561 ccgattccat tggcgatgga cttcatatcg gcttgacacc aggcattatc tggaaccttg
    16621 gtccctatac tttacgcgcc atggctcacc gccagagaag tgaagaccgc ggcggcacgc
    16681 cgggtcagaa gaaaggtgac agctggttga tcggtcatga tctttttctg tggagcccca
    16741 agggttttct gacgggctct gccaacacgc ccggatcaat tctactcggc acgcacttcg
    16801 agcgcgttga catggaggtg ggttgtaatg gcggcaccgg gatctcttgc gccagcgtcg
    16861 ttgctgccgg aggtagtgct cagctcaatc aattccaccg caacaggatt ctattgcgtg
    16921 aggcggatct ttggtacttc gtggcgccgc gcatgagcat cggcgtcaac tggctctggt
    16981 atgacgcttc gaacctgcgc aacggcaata accaggccgc ccataatcta ggtgtatgcg
    17041 acacccctgc tacccctgct aacggttgta gaaacggtaa aggcggagac tggcatacgc
    17101 tgttcttgaa ctggcgctat acgttctaaa cgcgtttaac gttcgcaacc gaaggggagt
    17161 cagaaatgac tccccttttt tttgcgggac tgaataccat ttgctattca ctatagcgct
    17221 ccttgcaaat tcgcctcgac gccgtattat ttccaagaca agccgcgtct gtaacaaagc
    17281 gcaaaaaagt tcaaaggata aggatagcta tgaacagttc actcataggt cgtagatccg
    17341 ttccagtgtt tttcacgatg attcttctga tggtctcgat cggctgcaac catgcgatcc
    17401 ccagattcgg ggtcggcggc cgctatgaag agggacgcga tcaattcttt cgcggcaagg
    17461 gcggtgacat ggaccgggcg gttgacgctt tggagtacgt cgttagccgt gatcccttgt
    17521 acaaggatag tttgacctat ttgggccgtg cttactatcg aaaaggacgt tatgaggacg
    17581 cctttgctat tttgcaacgg gccgtggcgg tcaacaagga cgatgaggtg gcctggattg
    17641 cgctgggact gacccagatg cgcctgggac aaaccgataa ggggctgggt tcgcttaagg
    17701 gcggcatcac cttggctcat aaggtgatgg tggagggata ccacaatcat caaaactggg
    17761 acagcagggg cgtgatccgc ggcgccctgc ggcgctctgc ttttcttttg gctaaaggtc
    17821 cggaggaaag agacaatatc ctccagacca atgaccggct tctagcactc gtggacgatg
    17881 aggaaaattt tcagaagaac gcccggctcc aaaacagtaa tcagatttac aattgatctt
    17941 tagggctctt ctccgccacc gctgtcgtta tcgcggtttc gatcgatggt ctcatcgcgg
    18001 ctggcacgat cgctctcgag ttccatttgt tttccgaccc attcaccggc ggcgataagc
    18061 ttcggccagt cgatatctct cccggaaatc atcgctaaat cggtctgaat ggccgagcgt
    18121 atttcacgcg cgggatccca gaacctgcca aatgaggcac cctggttctg atccacatat
    18181 tccagccatt catgcactcc acgcagacca ccttcaatct ccttgagacc tctctggcgg
    18241 tcgccctctc tagcaagcgc gaggccgaaa taaagcctgc taagatcttc ttcacgatta
    18301 gcggcaagcg ctcgctcaag tgcctcgcgc gcttgaggga ggcggcccat gtcgtaattc
    18361 acccggccga cgtaactcca aactccctga cgcagagctg tgccgtagac ataatttggg
    18421 tcgacttccg cagccctttg gaagtgtcca taggcgactt cattattgcc gctcaggtaa
    18481 gcctgtcttc ctgagttgac ctctcgagcg gcttggaaac cggcgcaacc tgaaaccagt
    18541 agaatcaacg acaacaagcc gattgaagaa aggtttttca taattcctcc tgatcggtaa
    18601 agataatccg gagcatctaa aaagacaatc cggctgctca gtgccgacca gccgtgcaat
    18661 gtaccgaagc tacgatggtg accgagttag atccaatatt tgctccagag taaatgatgt
    18721 tggccgcgtg attccacgat cgaataagca gctactccac cgcacgatct cttctcgaga
    18781 tagaagactc agcggactgg attacggttc ccggcctaag tatcaaatcc cagtagagtc
    18841 atccaaaccc gagcagcgat aagcgcgacc ataacgccgt aaatctgccg gagagttttg
    18901 gcggaaatct tgccgtggag cttttcaccg agtaccgcgc cgatagctgc tcccatgacg
    18961 acggcggagg tgagcagcag gggaatctgg ccggtcagaa tctttcctaa aaacccgctc
    19021 gaggtattca tcatcgcgat gaagagactg ctgccgatgg caatccgggt gggtactttc
    19081 aagacgtaga tcagtaaagg tacaaagacg aaattaccgg cgccgagtaa gccgatgaga
    19141 accccggcag ccagcgaata gagtgtcagc ggcaacaaag ctaccgacac ctgctctcgt
    19201 ggaagatcct cttgagcctg actgggtaca ggtaagaaca tcatggccaa gacaagaacg
    19261 gttatgatac caaagagcgc gagcaagaga tcttcggaaa ctttcgtcga taatagggct
    19321 ccggcgaatg agccgatcgc ggaactgggg ccggctccta aaacaatcgg ccggtgcacg
    19381 cgccgttggc gcaaatgagc catgccaccg atgatggtcg agaaaaatac ctggctgatc
    19441 accaccgcag cgaccgtgtt tgcgtccagc cgggatagac cgagcagcgg aggaatatag
    19501 tacagaagag gaaacatcat cacaccgcca ccgaagccca acaaaccgga aaaaaatccg
    19561 ccgatgactc ccagcgcggt tagagtcgag atgaaggcga ggctgatcat ccgacgctgg
    19621 tgccggaaac caaaacgtcg gccagtgtga tcaagaagta accgatactc acgtaagcat
    19681 tgaaatcgaa gaaagcgcga ttaatccggg atagatcatg aggccggaca atacgatgct
    19741 cccacattaa aatgaaagaa actgccaggc agccgatcca gtatatgcta ccgagatcgg
    19801 cagtaatacc gaccagaatc aggaagacaa tggtgcagag gtgaaagaag cttgagaggc
    19861 gcaatgccca aaccacgccg aacctagcag gaatcgaata caatccatgc gcgcgatcga
    19921 attcgcgatc ctgcagcgaa tagatgatat cgaaacccgc gagccaaaag accaccgcta
    19981 gaccgagaca aactggaatc agcaactcct tcaaacccaa tggcgaatca gtgaccgcca
    20041 gccaagcgcc gatgggcgca agcgaaagag cgatcccgag gaacaggtgt gatgcccagg
    20101 tgaaacgttt ggtcagtgaa taaaagaaaa gtaccgttaa ggccacagga gacagaacga
    20161 aacaagtcca gttgagttgg taggcggcaa agacaaatag tattgatgcg acgatcgtca
    20221 aaaatcccgc ttcccacggc ttgactgttc cgttcggcag ttcccaacct gccgtccgcg
    20281 gattgagcgc gtcgaatttc ttgtcggcca gacgattaaa acccatcgcg gcggtacgcg
    20341 cgccgaccat ggcgatcaat atccagaaga ctgttctcag agttatcgga tgatttggcc
    20401 aggcaagtac cagtgaagca aacgcaaaag gcaacgcaaa gatcgtatgg gaaaaacgga
    20461 cgagacttgc gtaattgcga attttttcga caagcattgc gatcaagacc gaaagctaag
    20521 tttgtcagtc tgtccgaatg ccgcaagaga attcacggca ggagcgtagg gaatgcgcat
    20581 ctcaaatgcc cagtttttgc caaatcgaat cgatataatt tttggtgttt tcgtccatga
    20641 cgatgacctc cggccattcg cggtcaaacc cttcgctgcg ccatttccgt gtcgcatcga
    20701 tgccgatctt tgacccgtag ccgagaaccg gggctgcatg gtccaagaca tccacgggac
    20761 cttcgacgat aaccgtgtca cgttggggat cgatgtggtt tcccgttacc caggtgacct
    20821 cggaaagatc atggacgttg acgtcatgat caacgattac cagaatcttg gtgaacatca
    20881 tctgacccat gccccagaga gcgtgcatga tcttgcgcgc atgggcgggg tattccttct
    20941 tgatcgagat gatcgccaga ttatggaata ccccgtgcac gggcaaattc atgtcgacaa
    21001 tctcgggaaa agtcagccgc aacagcggca gaaagattcg ttcggtcgcc tttcccatgt
    21061 aagcatcttc catgggcggg cgaccgacaa tggtcgttgg atagatcgga tgctcttttt
    21121 gggtgatgca agtgacgtgg aatacgggat agtcatccgc cagcgaataa aaaccggtat
    21181 gatcaccaaa agggccttcg cggcgcagcg gttcggcagg atcgacgtag ccctcgatta
    21241 cgatatcgga gtttgccggt acttcgacgt ccaccgtgac acccttgacc agtcgcacgc
    21301 ccttcttgcg taagaaaccg gtaaagagta tctcatcgat ttggtccggg agcggcgctg
    21361 tggcggcgta gatcattgcg gggtcgccgc ccagacagac agccaaatcc atgcgcccgc
    21421 tttgttcttt ctggtgctga taatggcgcg cgcctacttt gtgcaggtgc cagtgcatgc
    21481 cggttgtttg cgagtcaaaa acctgcatcc ggtaaagacc tacgttgcga gttcctttgc
    21541 ccgggtcttt tgaaaaaacc atcggcaagg ttataaacgg gccggcatct ccaggccagc
    21601 aggtgagcac gggcaacagg ctcaagtccg gttgccggtg aatgatctgc tggcattcgc
    21661 cgtcactgat aattttcggc gggatgccgg cgagttttag caaatctgaa aggagatgca
    21721 gcttgtcact gaaggatctc ggcggtctgg tttgcaccag tttttgaact tcgccggcaa
    21781 cctcctctac atcgtcgaca cccagggcga gagccatgcg tttttgcgag ccgaaggcat
    21841 tgatcaaaac cggaatctgt tttccgggga cgttctcaaa caatagcgcc ggaccgttgt
    21901 ttttcatcac gcgatcggca atctccgtga tctccaattc cgccttgacc ggatagttaa
    21961 tgcgcctgag ttcgccttgg cgttctaaaa cttgtacgaa gtcttgcaga ctgttatatg
    22021 ccatgtgctt cctgatctct ttcgaatgaa gagcaataag ccgacaagaa ggcgcaagtc
    22081 aagtaatcat ctgcgcggtg gcctcgcaat cgctacagtg atggggcttc cttggttttg
    22141 cccgtattta tctgtgtctt acgacctatg attgtcgacg tgggatcctg agacgatctg
    22201 atcgaatcga tactcgcctc cttgcaacgg atcaacacat ttcagcatta tcgttgagca
    22261 gtgacaacat ctcccagcgg ttcgcatcga acaggtcgca attctcgcgt agagagatca
    22321 atgaaaaagg cgtcgcgaca acagcaaaaa aaagctgaac ccgaaaaaac ccccggcact
    22381 gcagcgaaat cgcctgccag cgttgatagc ggaaatacgc gcttttggtt cctgactatt
    22441 tttgccgtcg cctttgctat tcggctcgtt tatctctttc agatcgctac gattccgctg
    22501 tttgagcatc tcgcgggaga tgcaaggact tattacgaat ggggccaacg gatcgcggcg
    22561 ggcgactggc tcggcacagg agttttctac caggctcctt tgtatccgta ttttctcgga
    22621 attctacaat tctttttcgg cgaaactctt tggctggtgc ggcttataca gattacgctt
    22681 ggggcggttt catgctcgtt gatctttttg atcggacaac agttgttctc acgtcaggcc
    22741 ggcataatct cgggattcat tcttgccagc tacggcccgg cactgttctt tgacggcctg
    22801 attgaaaaaa gcattctcga tctactcttg cttacagtta ttttgtttct cgttttttgt
    22861 gccggcggcg gactaacagt caagtggctg ggtgtcggcg cggtgctggg tctgctagcc
    22921 ttatcgcgcg aaaacgcgct tatcttggtc ccggtggtgc cgtgctggat tgcgctatca
    22981 tcgcgatcga tatctgcaac atcacggctg ccccagatcg gcttgttctt tgccgggttg
    23041 accctagttc tcctgcccgt cgggctgcgc aatcttgctt taggggggga attcaaacta
    23101 acgacgtcac aattggggcc aaacttttac ataggcaaca atccggctgc ggacggaact
    23161 tacaattcaa tcaggagcat catcggcgaa cctcaactcg aaggccgcga cgctaaacga
    23221 ttggcggaaa gagcgttgca acgcaatcta tccccgggcg aggtatcgaa ctactggtta
    23281 ggcaagtcgt tcgagtacat caaaaaggag ccagcagatt ggctgcgtct cctggccaag
    23341 aaatggactt tggtgtggaa cgcgcgcgag atcgaagatt cggacgattt ctacatctat
    23401 cgtcaatggt catcgttgct tagcgtgctc ggacggctga accattttgg cgttctggcg
    23461 cctctcgccg ctgccggtct cttacttaca ctcggacaat ggcgtcgctt gtggcttctc
    23521 tacgcgatga ttttggcgct tgccgcaagt gtcgcgggtt tttacgtatt tggccgttat
    23581 cggtttccgc tggtgccctt gctggttctt tttgccggcg ctggattgat agaactagtt
    23641 cgcttctata aacagggacg atggcggccg ctgctcgggg tgatggctgt attctgcgcc
    23701 tgcgcgattt ttactaactg gcccgtcttc ggatttagcg ggccaggtgc cgcgggttac
    23761 aacaatctat ccaatgctta ttacaaacag ggcaaggtcg agcaggctat cgagacggct
    23821 ctcaaggcta tcgaacgaga accgggctat ggcgtcgccc actacaatat cggcaacctc
    23881 tatgccggac aaggtaagat tgcactagcg caacgtcatt tcgaagaggc gctgcggatc
    23941 tatcccaatt acgccgaagt gcggtccaat cttggacagc ttctcgccga acaaggtgac
    24001 cttcaggctg ctatcgagca atttgaacga gcgatcgcgc tcgattcagg gctcttcagg
    24061 gctcatctca atctcggggt tgccttggcg aaacaagggc agatcgcaaa agcggatgct
    24121 ccgctgcgag aggccgcccg gctcaggccg caatccacgg aagctcgctt ttatctgggc
    24181 agtgtctatg cggctcagaa tcggttcgat ttggcggcgg agtcattcaa aaacgcgcta
    24241 cagatcgatc ctagcttggc ggccgcacac gagagtcttg ggcgcgtcct atcggtccag
    24301 gggaaaaaag aagaagccgt actgcactat caggaagcga tcaggctgat gagtggtaga
    24361 aaaggcggag ctaaaggatc cgtaacaatc cgctaacgag tcctcgggta agagctgagt
    24421 tctacgtcat gttcagggca cgattgtcag aatgaaagac cgcggcggat ccaccacctt
    24481 gatgaacggg atcggatcgt cataaatctt aaccgggatg atggttgctg ctgggttgct
    24541 tatcagcccg tccagccaat ggtatttcaa aataacttca ctagctgcgg cggtcgagac
    24601 ttccaggcga ttgagtttgg cgacgacttg accgtgccct tgtaagaacc aatacggcgg
    24661 ctggttcacg cgcatcagat gcaccgggcc tagccgccgg tccagcgtta ttagtccagg
    24721 aatagatcgt agccgtttga ctgaagccgg gtgaaaggca accaccgcgc cgatgttgta
    24781 gagccgaaaa tactgtttga tctcttcatc actaaaagcc tgtatatctc gtccgaataa
    24841 tctgcccgag tgaaattccg cgaagtggtg ccggtcgtta tagagattga tgggcccgcc
    24901 gatcagctcg cggccgctcc aatgcggaat aaaagcggat aagtacatgc catcgtatac
    24961 gaaacccgtt tcatcgcctg attcttcaaa cagaacccgg gcctccttgg gcgtgtcttg
    25021 tcgcacccag cctactatct cggtgatctc aggacggacg agcgttcgca gcctgagttt
    25081 cgcttgagac tcggtaccga cgagattgat caggaaagtg atgctcgcgg taaacagcaa
    25141 aaacggaact gtataggaat agaaagacga ttttgttgaa cccgccccca gccagtcggc
    25201 aagcgcgtaa gccgcgccaa cggttaagaa aagatcgaag ggaactttaa atcgcaacgg
    25261 ctgccatgcc tgcagaggcg gaataaaaaa actaaaataa gtgattagga acagactgat
    25321 cagcgcggca ccgagcaaaa tagccacatt tttatcgcgg gagcgcagca gcgtcaccgt
    25381 gcctgttgtg ccgagaatca gcagcagtaa tcgaaagcct ttctcccaaa atgactggcg
    25441 aaatgaccag aaattcttcg gcgcgaaata gtccttgatc aatgtcaggg gatcggtact
    25501 cacaaatagc ggcagctgct cgacaatcgt tttggaaaca tcgtcgcccc ggtggctgaa
    25561 agcgggcatt atccaaacca agtttagcga caacgatatc gcggcggcgc cggccgtcca
    25621 tagcgccagg tcgcgttgca tgagcttggg ttgcgcgatt aacaaggcga gaatgggcgg
    25681 cacaaaaatg acaagcgatt gaacgtggag cggtaggatc gcgattgcaa agaagatcca
    25741 gccgaggtgg acagcgcccc aggaagtttg ctgtcgggtc agacggtaaa atagcgaaac
    25801 tcccaaaccg ctggcataag cggccgtcga gtagccgatc atgccgtaaa aaaacatttc
    25861 tcgaggcagg gaattccacc aataaatcgt tccaagaaga gcggccaaca cggccacagc
    25921 atttctggtt tcttcgcgcg cgaacagatt gcgcgcgctg aaatacatca gccacgggac
    25981 cgccgccatc gcgataaagg ccgaaatttt aaaccactgg atcggcgtta aagcgatcaa
    26041 cgacaatgca agagcaagga actcgaacag tttgatgctt agatcttgga tggtattgga
    26101 cgggtagccc gccatgaaga gcgggttata gccccacgtt gacttgtcct gccaaaatgc
    26161 ctctagggat ttgagatggt ggaaatgcaa cccccagtcc tgatcgatga tcggatcagg
    26221 attgtataga ccggcaaaaa catcgaagag acagatcaac ccaaacgcat ggagcgcgga
    26281 gattgcgacg atcacaatca ccgggcgttc aaaaagccgt ccatgaggac ccgtaagtag
    26341 ccgtctgtgg gggttagcta acgatccggt ggcgcttacg gcagaatcgc gcttgtcttg
    26401 ccgggaaatt ttgcgggttt tatgtttagc tggcacgtac ggggactata ctatcgataa
    26461 tcggacggaa aaaacaaaat caacccagga tatccatcag ctcgggtgtt gtctcgtagt
    26521 ctgcaaggta caatctcgta gaacacgggg ctgggtggca tccgtcacgg ctcccagcgt
    26581 tcttcctccc gcccgaacgc aattccgccg ccggactcga tcagcttggt tcgaactggg
    26641 cgcttcgcca gagcgattct tacgcgatca tttgcatccc aagatcaagt ttgtcagaag
    26701 agacggatgc ttggtgtagc tctcgtgctc ggattcttga ctgcgctagc caatatcttg
    26761 ggcagtgctc ttgcggtgct ccagcgtgag ccctcgcggc gctttaccgc ggcggcgctt
    26821 gggttaagcg gcggatttat tctggcagcg gcgctcctcg aaatgttgcc ggaaagctta
    26881 tcacggggac cgtcgatgcc cggttatgtc gctgtcggat acctctctct atttctgatc
    26941 gagcaattta ctaatgtcca cctgcatagt ctgccggatg atgaacatcg gatgcctgtg
    27001 ccggtagctg ccggatttgc atctctcgtt gctttcaacg tccatgactt tatcgatgga
    27061 ctggctatcg gcgcgggcat gatcgccaaa accgatctgg gaatattagt atttctctcg
    27121 gtgctacttc atgaaattcc ggccgggttc gtgatcgctg cgcttatgcg cgcagcaggc
    27181 tggagccggt ggtctgctat ggttgccggc ggcagcttag gggttgtcac gatccttggt
    27241 atcgtcctgc cattttgggc ccgggaactt aaaccgttct ttgccgaagc cctattggca
    27301 ttcgctgccg gaaactttat ctatgtgggt gcgactctgc tcgtgccgct ctccgagtcc
    27361 ggtaaatcgc gctggatcac gctcctggtg gtgctcgggt ttgctatttt ctacgtttcg
    27421 agccggctcg ttgagtcctt tttctagatc gcatttattc ttgtgaccaa aaaaggggga
    27481 gtactatgtt tttaaaatta ctgtgcgcaa ccatgctagt gatcttggcg gcggcctgtt
    27541 cgatgtcgcc cgaacagccg tctcgagcga cggccgaact aaaagacaag gacggcaagg
    27601 ccgtcggggt ggctacgttt cgcgagagct ccgggggagt gacggtcgag gttaatgtca
    27661 aaggactcac gccgggcttg catgcggttc atgtgcacgc agtcggacgg tgtgatgcgc
    27721 cggcgtttac cagcgcaggc ggtcatttca atccggcgca aaaaaaacat ggctataaaa
    27781 gtcccgaagg cgcgcacgcc ggcgatctgc ccaatatgct ggtcgccaag gacggcacag
    27841 ggcgttttga atctttcacc gatggcatga cgcttaaacc tggcgcgaca tcggttttcg
    27901 acaaagacgg gagcgcgttg gtgattcatg ccggtgtcga cgataacgtc accgatccta
    27961 ccggcaatgc cggcgatcga gccgcgtgcg gcatcattac aatggctcaa tccagaatgt
    28021 agcaggacta gagataatgt agcctggtcc cggcggccag gctgcgctcc gcttcctgga
    28081 tgcggtcggc agttccaaga tcctgccaaa atccatcaaa gcaaaaccca tagagaggtt
    28141 gcccgtccaa gagcatccgc gggtaggttt cgcgggtggt gctgaatttg tgcgccgtca
    28201 ttccgttatc catgtagtcg aagaccgtcg gctccaggac ttggacgcct gtaaacatca
    28261 atttgcgaat cggcccgggc gactgcgccg gcgttctcgt gtcaagaaat cgacggatac
    28321 gaccttgctc gtcgatgtcc atcgagccgt atcgatccgc atcgctatca ggccgcagaa
    28381 ccaaagttgc cgcagccttc tgtttctcat gaaacgtcag caattggtcg agatgcaagt
    28441 cgatcaacac atcggtgttg atgacgatga aagtgttgct ttgcaagaat ggcttggcct
    28501 tgagcagacc gccgccggta tccaacagct ccggctcgag cgaataactg atctgcaatc
    28561 caagctggct gccatcggcc agccgggtct cgatttgatc accatgatga tgaaggttga
    28621 tgacgatatc gcggatgccg tagtgtcgta agagcaggag ggagtattcg atcatcggcc
    28681 gacctcccac cgagacgaga gccttgggcc gggtatcggt cagcggccgc agccgtgtcc
    28741 cctgtccggc ggctaaaacc atcgctttca tcgcatctcc tcatagtgct cagccaaaag
    28801 cggaacgatc ggttcgagca acggcacgcg cgccaagttc cgtttcaagc gacgcaccgt
    28861 aggcgggatg aatttccggt atgccggttt acccttcaca agatcgaggt aataaaaccg
    28921 gccgacgact tttaagtcgc gctgaatcgc tgagagcaga taaatctctc ggaaatcgtc
    28981 agcgtctata gcgcgctttt cgatgtcggc tctccggcgt aaataataac tcacaagctg
    29041 atcttccatg tgcggcagga tcacgctgtc ggtgatccgg tcgtttagca gcgaagccag
    29101 atcgtactgc ggcggcgcga gcaaggcgtc ttgaaaatcg atcagtgcga cttctttgtc
    29161 gtggatcatc aggttccaac tgtggtagtc gcgatgattt aaacagctcg gataccgatc
    29221 cagacgatgg gcaatcttcg agaatattct tcgcagcgtc tcgatcccgg ttcctgccac
    29281 atagacgccg ggccgcttct ccaatccgta ttcgatgaaa tgatcgaatt cccacatgta
    29341 gagccggaaa tcgaaacgct gctgaaatgc gacgcaagcg tcgtcgcgtg cctttgtccc
    29401 tcggatttgc agaaccaaaa gctcgtcgat agctttctga taccatgtca ggacctcatc
    29461 ttcgggcagg ccttggatgc ggtcccataa cgctgtgtcg cctaaatcct ccagcagaag
    29521 aatgccgtcg ttttgccaat gaccgtaaag ttcgggcacc cgcacgccga ttccgctaag
    29581 aaatcggtgc aaattgagga aaggcaattc tttaggagcg tgcttaaaga ccgctagctc
    29641 ttcactggag agcggcagag cgctccctgc cggtaattcc atgacgatca ctgatttcgg
    29701 cgcgcctgaa gcctctatga cggctcggta gtaacgccgg ctcgacgcat cccctgctag
    29761 cggcacgagc gaagacacag ccgtgtttgc gccgaaaacg gcgccgatct tatctgagat
    29821 aattttctgg tgatccattt agatgtgcaa ttctattctg ccggcgccgc cggccattaa
    29881 acttctcaac tgccgccgaa accgatggtg accttgccgg cctcgacaat caccgggatc
    29941 tggcggcggc cgttggagag cttgagcata cgcgccaggc cgtcgtcatc ctcgagcacg
    30001 ttgatgtatt cgaaagatat ttttcgccgg gagaaatcct cacgggctcc cgaagtatag
    30061 ggtcagagat cttggccaaa gataagaacc ggttcgctgc tcatcgtctt tgctcctgct
    30121 aggttgataa acagatatta ctatgaaccg ataaattctg catgtttcag atcaccgcaa
    30181 attgaaataa gcttgtgctg cgtgttaggg atgggtactt tatgcccggc actctgtaca
    30241 tcgtcgccac gccaatcggt aatctcgaag acatcacaca acgagcaatt cgcacgctga
    30301 aggaggcgga tatcatcgcc gccgaagaca ctcggcacac gcaaattcta ctaagccagt
    30361 tcaccatcga cacgcctctg acgagttatc acgagcacaa cgagcgagcc aaggcgggcc
    30421 aattggtcgc tcgcctcgag cgcggccaaa gcattgccct ggtctcggac gccggcacac
    30481 cggcgatatc cgatccgggc tatcgcttaa ttgtcgaggc aattcggatg ggaattcggg
    30541 tggttccgat tcccggtccg tcagcactga tcgccgctct cagcgccagc ggtctgccta
    30601 ccgacggatt taatttcaga ggatttctac cttcacgcaa gcgcgagcgg aggtccaggc
    30661 tgcaagagct ccgcagcgac cgttatagca tcgtagttta cgagacgccg catcgtctca
    30721 aagaatcgct cgatgacatt cgtgaaattc ttggcgaccg ccggatggtg ctggcgcgcg
    30781 aggtgagcaa aattcatgag gagttcctcc gcggccgcgt cagcgaagtg atcggtgaag
    30841 tccgtcatcg agaaattcgc ggcgaagtga ctcttgtcat agaaggctgt tccgatctgg
    30901 atcctccttc agaagatgtc ctgagcgagg agattgctag gctcatagcc gatgggatgc
    30961 gggtaaaaga gattgccgag gtgctcggcg agaaatacgg ctattctaaa cgccagatct
    31021 acgctttagc tatggatcaa gccagaagca ctaactcttg accttgtact cttctaattt
    31081 tcgataaagg gttgtgcgat ccacgccgag tatccgagcc gcttcggcct tattgccata
    31141 cgtgctctcg atgacgcgca aaatgtactc tctctccaga tctttgaggg tgtatccgtt
    31201 tgccacagcg gccgcgagcg gcacggaact tccgttgcta gcctgcgacg gcaacggtaa
    31261 atccttcaag ccgatgctcg tttctttgcc aagcagcacc gctcgttcga tggtgtgttc
    31321 caattctcgc acatttcccg gccagcgata atttactagc ccggccagtg cctcccgatc
    31381 aatggaggct atgtctcgtt tcgcctgagg cgcaaatttg tgcagaaagt gctctgccag
    31441 aagcggaata tcttccgacc gctcgcgcaa cggcggcaat tcgatgcgga tcacatttag
    31501 ccgatagtag aggtcctgcc ggaatctacc ttgttcgacc agatgttcca aatcgaggtt
    31561 gcaggccgag acgatgcgcg tgtccactct ttccgactga tttgccccca agggccttac
    31621 ttccttatct tcgatgacgc gcaacagctt ggcttgcagg ttgatgggaa tctcgctgat
    31681 ctcgtcgagg aaaaggatgc cgccgctggc ttcctgaaac agtccgcgcc gatcttttcg
    31741 cgcgtcggta aacgcgccgc gtaaatagcc aaagagctcg ctttcaagca gcgtttccgg
    31801 aacggcagcg cagttaagcg gtataaacgg cccttcggcg cgtgagctat gatgatgaat
    31861 ggcgcgggca accatttcct tgccggtgcc gctttcaccc aggatgagga cattggcggc
    31921 aagatcgctg gtttgccgta taattgagaa aacctcctgc atcttctcgc tcttgcctat
    31981 aatgttctcg aagctgttgc gctccagtac ctcgctccga agacgctgca gttcttgaca
    32041 gagccggcgc tgttcgaatg cgcggctcac atgcaagatc atttcatcgg tgcgaaaggg
    32101 tttcgtgaca taatgataag cgcctgcttt cattgcctcg accgcgctct caatgctgcc
    32161 gaacgccgtg atcaaaagta tcagcgcgtc cggatcgatg gctttaactt cttttaccag
    32221 ttccgtgccg cgcatccgcg gcatcatcat atcggtgatc atcaagtcgc agccggctgc
    32281 gcgaaacttc tccagcgctg atcggccatc atgcgccgcc tcaacgtcat agccttcctc
    32341 ggccaacaca tcgaccagca gctggcacat ctgtcgatca tcatcgacaa tcagaatacg
    32401 gccgtgcttg ccggcgtgca gtttagaccc gggtgccatt ttccagattg ccctgcaccg
    32461 gcgcttcctt ctgctgccct agaggcagat ggatgatgaa gcgggtaaac tttgcaggtt
    32521 cgctttccac gatgatctca ccgttatgct ctttgataat gccgtaagtc actgccagac
    32581 cgagtccggc tccttctccg acatccttgg ttgtgaaaaa cgggtcgaat acatgatcga
    32641 tggcttccgg cgcgatgccg attccgttgt cttcaaagat gatatgaacg ctgccgtggc
    32701 cgccgacaaa aggcatggct gaggcctgaa ttttaatcac tcctccaggg ccaagcgcgt
    32761 ggagagaatt agcgaacaga ttgatgaaca cttgttgaag cagatcgggg tcggcttgaa
    32821 tgaataaggg cgatggcgag acatggattt cgacatcgag cttcttttcg aggagttggt
    32881 gttccaaaag gtagcggaca tgattgagca attccgataa ctcgacggat cggaacatcg
    32941 gttccctgcg ccgagaaaat tccaggagtt gccgcacgat ggcggtaatg cgatcgcttt
    33001 gggagcggat cacctcgagg ttgccggcaa gttcctcgct gctgcgcggc cgccgcaaca
    33061 ggtattcagc ccggccgcga acaatgctca atggagtgcc gatctcgtgc gcgagccccg
    33121 ccgccagttg acctaccgat gccaagcgtt cggagtggcg caggtcccgt tctagattta
    33181 gcttttgttg ctgctcttcc aagaacttgg aataactccg attgagctct tccgacatgc
    33241 tattgaactc tccagctaac gcggcaattt catcgcgacc cttgatctcg atgcgctgct
    33301 cccaacgacc tctgccgatc tcccggacac gttccaccag ctgattgatg ggacgcgtga
    33361 cattgcggcg cacgatgatc agaatgagaa ccgacaaaag gatgaccaat gccgcggtag
    33421 ttccgatgat tcgctgccgt cgcgcttcga tgttgctaat caattgagat ccctgacgcg
    33481 ctaagacaaa gaccccggca gtccggccat cgctcgaggt aaacggttcg atacggtagt
    33541 acaccaaaat accaggttcg cgaatatacc catctatgcc gttgggttct gcgagagccg
    33601 gtcgcggatc cagtttcaga atggcagtag gatccagttc gtcaaaatcg ttcggatatc
    33661 taagtgaaga ggaaagcacg accggctcgg cattaaggtt gtaaacaatc aggccatgaa
    33721 tgttgccccg cggtcccacc gtgttggcga actcctgagt cgcctgcaga ttttgaccgt
    33781 cggcatacaa gtcccggaga gctgagtgga tggcgcggga aaagccgcgc atgccgaccc
    33841 gtatttctcg cgtgacgttc tcacggtcct gctgaatgct caaatagccg tgaaccgtca
    33901 ttgtcaccac gatgatgacg accagcgaaa caattaatct agtgccaagt ttcatgggcg
    33961 ctgcttcgta cgcggttgag caagccttat accgtgtcgg atgcaaagcg taattgcatt
    34021 aaaaatcgaa ctctcgggat gttcacgaaa aaacatggag caactctgtg gcaatttacg
    34081 acagcatgcg gtttttcaca atcatcgaaa tttagctcgt tcattcaggc aattgagctt
    34141 ctttcgcaga ggcgcacgca cttctcacgt tgcatattcc aacggctaga accgccaaga
    34201 tgcgcgttta ttttgagaat gtcggtgacg cctcgacggc acgacgcttg cgtttagggg
    34261 ggcgatacca gcgcctaaca aaataacaaa ctctcagatc cgaaaactta gaaaggagga
    34321 gccgatatga aaaagctact ttttgcattc ctgcttggac tgaccgtcgt gagctcgatc
    34381 gttgatcttg ctacgccggt gagcgcccaa gacgaggatc ccaaaccaag gaagcccgaa
    34441 ggggattgat cgggagaagt tgatttccca gttgcaaaag ggggctttgt gccccctttt
    34501 tttatacttg atgatgacgt cgtcttaatc aagcggccgc ttgatgcgaa atccactaaa
    34561 ctcgccgcga aagtcctgcc gctgcagatc tacttcgtcg acgcgagctc ccggcggtcc
    34621 ctgccggcac cagacgatga gctcttcgat acgatccggt cggccttcgg ctaccatctc
    34681 gacggaacca tcatagcgat tcatgaccca gccggttagg cccaagtggc gcgcgcactg
    34741 aactgtcgaa gcgcgaaaat agacaccctg cactcgacct ttcacaatta gccggatacg
    34801 cgtctgggag ggagtcgttg acatgactgc cggttttcta acgcggtgcc tgttctgacg
    34861 caagcgtcat gcagcaatgc ccgcccaaaa ttatttgaca agcgccccgc agtttcggta
    34921 ggctttcgcc ggttctaagg gtttaccgaa gagcccgcgc cgtggtcctc cagaccgatt
    34981 taggccctgc cctttggcga ctcgatttgc tcagatgcgc actcgtgaat caaaaaggag
    35041 ggtctgatgg gagccaaatt atatgtcggc aacctgccgt actcggtgac ggaggagaga
    35101 ttgcagcagc attttgcgca gcaaggctcg gtgattagcg ctcgtatcgt taccgataaa
    35161 ttcacgggcc gttccaaagg ttttggcttc gtcgagatga gttcggatgg cgaagccgaa
    35221 cgcgccatca atgctctgaa cggaaccgat ttcgaaggac gtaacgttgt cgtcagcgaa
    35281 gcccgtcctc aggcgccgcg agattctgga ttcggtggcg gcggaagcgc cggcagacca
    35341 cgtcgggatc gttactgatc aggtgtcagc agcttcagga gctcttgttc ctcgatgatg
    35401 cgaacgccga gctctcgagc ctttttgagc ttggagccgg gctcggagcc ggcgacaaca
    35461 tagctgctat tgcggctgac tgcgctgctt actcgcccgc ctagagcctc gatccggcgc
    35521 tgcgcttcca ccctcgacat agtctcgagt ccgccggtaa ggacgaatgt cgaacccgaa
    35581 agcgcgcctt gtttgatagt ctctgccttg aagctaatgc cggaatgcaa aagcttcgcg
    35641 atcacttgac ggttttcgtc ttgggagaaa aagcgcgcga tgcttttggc gacctctggg
    35701 ccgatctcgc gcacctccat cagtttctcc tcgcttgcat tcataatcgt attcaggtcg
    35761 ccgaagtgcg cggtcaaaag tttggccgtg gcttcgccga cgtgccgaat gcccagagca
    35821 gtcagaaaac gagtgagagt cgaatccttg ctgcgctgca aagccgcgag caaattttgc
    35881 gcggattttt tggccatgcg atccaagctc gccaatcgct cttctgtcag gctgtaaaga
    35941 tcaccgagat ccttgaccgc gccgcgttcg accagttgat cgatcagctt gtcaccgagc
    36001 ccctcgatat ccatggcgtt gcgcgacccg aaaaacttta ggctttcctt gagctgtgcc
    36061 ggacacgaga taccgatgca gcggtatgcc gcctcgcctt cttcgcggta tactgccgcg
    36121 ccgcataccg ggcagtgctc gggcatgcaa aaatgtttct cttgcccggt tcttttctcc
    36181 gtaatcactt taaccacata aggaatgaca tcgccggcgc gctcgaccac gaccgtgtcg
    36241 ccgacgcgaa tgtctttgcg gttgatctcg tccatgttgt gcaatgaagc gcttttgacc
    36301 atgacgccgc cgatggcgac gggttccagg cttgcgaccg gagtcaacgt gccggtgcgg
    36361 ccgacctggg gttgaatctc gataatacgc gttgtcgcct ggcgcggttt gaacttatag
    36421 gcaatcgccc agcgcggcga ccgggcaatc tcgccaagcc gtcgttgcag ttcgtgccga
    36481 ttgaccttga ctaccaagcc atcgatctcg tacggaagtt catcgcgccg gctctccatc
    36541 tcgtcgcgaa attttaggat atcttcaatg ccacggcaga cgcgcgcaaa aggcaccggc
    36601 tttaaacccc agtcctgaat cgccgcgcga aattcagcgt ggctggtcaa gcttgctccg
    36661 gtgatgactc ccgcgccatg gaaaaagaca tcaagcggcc gtttcgcggt caatgttgaa
    36721 tcgagttgtt ttaacgagcc agccgctgca ttacggggat tggcgaagac cgcttgcccc
    36781 ccttcctcgc gttcgcggtt catccgctgg aaggctgcgc gcgaaagaaa aacctcgccc
    36841 cggacttcaa gccgctgcgg caccgggcgg gctccggcgc gcaagagcag cgggatcgat
    36901 cgaatggttt tcagattgag cgtgatatct tccccattga tgccatcgcc gcgagtggaa
    36961 ccagcagtta accggccctc ggcgtagact agctccaccg ccacgccgtc gattttgggc
    37021 tccaccaagt attcgatggg ttcggtggcc cttagaaatc tcactatccg gtcttcgaac
    37081 tctgccaaat ctttttgatc gttggcattg ctgagagaca gcatcggcag cgtgtgccgc
    37141 acggtcctga atttgtccag aggaggagcg ccgatttttc gggtcggtga atccggtgaa
    37201 gcaaattgcg ggtgagctgc ttcgagctcg atcagacgtt ggaatagccg gtcgtattcg
    37261 gcgtcactga tcaacggatc atcgagcaca tggtattgat gattgtgttt ctcgagctgc
    37321 tgtcgcaaca cgatgatctc gttttcggcc gcaatttcaa gattctttgc cataggacca
    37381 actagtgaaa ctttactcgc ccaaccaaaa cgaattagca taattgtcac gctgactgaa
    37441 gctcgcgcga gcttacttca ttgaccggct ctggtgagta tgttaaccac cttgataatg
    37501 agaacacggt tttacggaag cgctatctgg cttttgatat tgtccggttg cgcctcttcc
    37561 ggtgccgtgc tcaaagctcc cgccaacggg ctgttcggat cccctgaagc ccctttcctg
    37621 cagcagcaac gagtcgaggt cccggctgag gccagaggtc tttcgcattt tatgaaaggc
    37681 cacctgctgc ttggcgaggg cagattcgat gaagcgctcg aagaattcga gagcgcttcg
    37741 caagccaatc cgcaagatgc gttcctgcgc tttcgactgg ccacgcttta cgtcaggaaa
    37801 ggcgatctta aacgagcgct ggtggaagct gaggcggcaa agagaatgga tccgaacagt
    37861 gtcgaaaacc gtttgctgct ggcaggcctc tattcatctc tgggagagaa tcagaagggc
    37921 atcgccgaat atcaggaagt tctcaaactc gatcccaaaa accaggaggc tctgctctac
    37981 ctcggcgcgc tctacttaca aaacgacgac aactctcgcg ccactgaaat gctcgatacg
    38041 ctgctgaagc tcgacaacga ctcggtgctc gggcactact atctcgggcg tgtgcgcgcc
    38101 aagagcaagc tctattcagc ggccgagcag agctatcgca gagccttgga gttgaatccc
    38161 aagtctgaag cggtgcttat ggatctggcg ctggtctatg aactgcaaag caagagtgaa
    38221 gatgccattc agacttacca aagattgctc gggctgaacg cgcagaatgt ctgggcgcga
    38281 aaacggcttg gcgaactgta cgtcggccaa aacaaactcg atgatgctct ggcgcaattt
    38341 gaaaatttga aaagcagcga agcggatccg cgcgaaacac ggatcaaaat cggtctcatc
    38401 ttctatgaaa agggcgactt cgaacgggcc gcgaccgagt tcaatctggt cctggccggc
    38461 gatcccgaga acgatcgggc gcggtattat ctcggcgcca cctacgtcga gttaaagtcg
    38521 gatgaaaagg ctctcggcga atttgccaag attcccgaga agagcgacct gtacttagac
    38581 agtcgtttgc agatggctta tctctatgaa cgcacggacc agttacaaaa agccatcgat
    38641 tcggttcaat cggtgctccg caaaaaaaat gaccgcaagg aagtctacgc gtttctttca
    38701 accttgtacc gcaaagccaa ggaattcccc aaagccatcg aggcaatgga gcgcgtcatc
    38761 gtcctggacc cgaagagtga tcaggcctat tttcagttgg gagcgctgca cgacgaggcc
    38821 aagaacaaag aggaaagcgt cgtcaacatg aaacgggcca tcgagctcaa tcccaagaac
    38881 gccgcggcgc taaactatct tggctacacc tgggccgaga tgggcgtgcg cctagatgag
    38941 gcggaacagt tgatccagcg cgcattgcag cttgagccta acgacgggtt ttatatcgac
    39001 agcctcggct gggtatactt tcagaaaggc gactacgcca gggccgtcga acagttagaa
    39061 cgcgcggtgg cgatcacggt tgacgatccg accatcatcg agcatctcgg cgatgcttac
    39121 ctaaagtttg gaaatttcga tcttgccttg tcgcgctatc gcgaggcgat tcagaaatcc
    39181 aaagaagatg atcaagtcaa acgaatccgt gataagatcg agcacttgga gaaaaaaatt
    39241 tgagggcggc tctgcggcgc gaattatccg ttcgcgtgca gaatttcgtt ttcttgttcg
    39301 cggccgccgt tttcacgatt aacggctgtg ccaccgttaa accgccgccg gcgaagccag
    39361 agcttccggc caaacagtgg caaaccggcg agctgatcca ctcgctaggc gagcggcaaa
    39421 agcaattccg ttcgttgcgt gctttggcgc gccttgatta tgccgggccg gacggtaagg
    39481 gcaatgtgca agaagcagtg ttggttcagc ggccggaccg gctacggctg gaaaccctga
    39541 ccttcctcgg cgctgttttg atcgttaccg tcaatgaccg ggagatcatc ggctatcacc
    39601 cgcgcgaggg catttttgtg cgcggtccgc gaaacaagga aaatcttttt cgctatacgc
    39661 aaattcctct ggaactagat gaaatgaccg cgttactgtt gggcttgccg ccggttgaca
    39721 caggcgcgcc ggtgcgtcag gagggtccgg ctctggtgtt ttcgccaaac ggactcaagc
    39781 aggatgcggt cgcattcgag gcgcaagccc ccctgccgac caaatgggaa cgctttaacg
    39841 acgccggcgc cgtggagtta agcgcgcagt tcatggacta cgttttgacg ccggctggcc
    39901 catttccatc gaggatccaa atcgaagcag agctgcagaa gagaaagctc gatatccgct
    39961 atcaacagcc cgaggtcaat gcgtctctcg cgccggaaat ctttacacaa gaaaagccgg
    40021 ctcacgctga ggaactgcgg ctggagacac taggccatta gggatgtttg tcgcgcgtgg
    40081 tgttggagtc ctgttacttt gtttgctgct ggcaggatgt cgtgaccagg ccgagatcac
    40141 cgtcggtgac tcgcaaaaca tcgttgatga caaaaacgcc ggcgcgccga caacgggtga
    40201 ctggctgatc atccacgccc tcagcgaccc tgaacagctc aatccgctca ccagcaatga
    40261 cgccggttcc agcgaaatca acggttatat cttcgagagt ctcttaagcc gcgacccgcg
    40321 tactctggag ttgaagccga tcatcgcaga agcgagacca gagatttcgc aggataaact
    40381 tagctacact ttcaaaattc gaaagaatgc gcggtttcaa gacgggcgtc cggttaccgg
    40441 tgaagatgtc ctatttagcg tcaaagcgat caagtgcccg ttggtcaacg cgccctttct
    40501 aagggtctat ttcaattccg tcgtcgatgc gcagcttctc gatccttaca cgatccgatt
    40561 tttcatcaga gaaccctatt ttctaaatga gagtgtgctg ggcggggtga ttgtgatgcc
    40621 gcgtcattac tacgaccgag agaatctttt gcgctcgatc acggtccggc agctcgcgca
    40681 agatccgacg aagctgcccg ctgaggtaaa gaaatttgcc gacgtgttta atcgtaatta
    40741 caaccgtaat ccgctcggtt ccggaccgta taagttcagt cactggaaga ccggcagcga
    40801 aaccgaactg acgcgcgaca ccgattattg gggcaacggt caagaaggaa tcgaccagcc
    40861 gcacgtcgat aaactcaaat ttcgcgtggt caacaacatg gacgcggcgc tcacgcgtct
    40921 caagagcggc agtttggatt atatggagag tttgcagccg gtgcaggtgg tgcgcgggac
    40981 gagcagcgaa cgatttaagc gagaattcaa aaaatacgaa tattacgcgc ctacctacac
    41041 ctatatcgga tggaataacg atcatccgat atttggcgac aaacgagtgc gccaggcaat
    41101 gacgtatctc accaatcgca agcaaatcgt cgataccgtg ctcttcggat tgggcgaggt
    41161 ggttgatagc ccgatttatt tttttcggcc ggaatatgac aaaactctag tgagttatcc
    41221 cttcgatccc gccaaggcgc tcgccttgct caa
//
LOCUS       DASUVS010000209        20647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1834203, whole genome shotgun sequence.
ACCESSION   DASUVS010000209 DASUVS010000000
VERSION     DASUVS010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 20647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20647
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1834203"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..461
                     /locus_tag="VF182_11760"
     CDS             6..461
                     /locus_tag="VF182_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog family protein"
                     /protein_id="HEX6437800.1"
                     /translation="MSWPDIRSLIPHSGAMVLLDRVIAIDEETLCAEVLIGPDSLFGS
                     AEGVGAWVGLEYMAQTIAAFAGYTASLRGEAVKPGFLLGARRYECTLPVFSVGSLLKV
                     HVRRVLQSENGLGSFQCRIEHGEEEVATAMLTVFQPADARDFVKGDSNE"
     gene            454..1179
                     /gene="fabG"
                     /locus_tag="VF182_11765"
     CDS             454..1179
                     /gene="fabG"
                     /locus_tag="VF182_11765"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX6437801.1"
                     /translation="MNKTVLVTGSSRGIGRAIAYRLAQDGYDIVAHCRVRRQEADELG
                     RSIVALGRQARVLQFDIGDRAAAAATLEADVERHGCYYGIVCNAGVARDSVFPGMSGE
                     DWDVVLRTNLDGFYNVLNPLIMPLVRRRAPGRIVTLASVSGLIGNRGQVNYSAAKAGI
                     IGATKALALELATREITVNCVAPGLIETEMTGQAPVDEILKLIPAGRTGTPDEVAAAV
                     SFLLSDGAAYITRQVMSVNGGLI"
     gene            1176..2405
                     /locus_tag="VF182_11770"
     CDS             1176..2405
                     /locus_tag="VF182_11770"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEX6437802.1"
                     /translation="MRRRVVVTGMAGISPIGNHWTAIRARLESYRNAVMRMPEWSDYD
                     GLNTLLGAPAAPFELSERYTSKATRSMGRVALMATRASELALLDAGLLDDPLLKSGHL
                     GIAYGSSAGTPKAICDFGKMIDEKSTKGINATTYIKMMGHTAPVNLGVFLGITGRIVT
                     TSSACTSGSQGIGYAYETIRSGRQRAMIAGGAEELCVTEVAVFDTLFATSARNDAAHS
                     TPRPFDAERDGLVLGEGAGSLVLENLEHARARGAKIFAEVVGFGTNSDGSHITQPNAR
                     TMQIAMELSLADADLSPSAIGYINAHGTATQQGDVAESQATRQVFGGNVPISSLKSYM
                     GHTLGACGALESWISIEMMRAAWFAPTINLAQIDPHCADLDYIVGAGRQLECEYVMSN
                     NFAFGGINTSLIFKRYD"
     gene            2487..2933
                     /locus_tag="VF182_11775"
     CDS             2487..2933
                     /locus_tag="VF182_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ATPase subunit"
                     /protein_id="HEX6437803.1"
                     /translation="MANVVSMAILTLCLAIGLPAHARDTRHVLSIAVAMETKDALDKL
                     DGSVRFYFGDQSAPQVLAMLGSDVSNRKTNAFGKSDEKACNWAFLSAMLALEKRAHQM
                     GANAVINIVSYYNKKVMSSATEFECHAGGIMAGVALKGDFVKITDG"
     gene            2926..3633
                     /locus_tag="VF182_11780"
     CDS             2926..3633
                     /locus_tag="VF182_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEX6437804.1"
                     /translation="MDERNSRAATVWLLDIRRVPECVVNYFVQRLAASEFHRYAKFMR
                     SQRKRQFLLARMLLRLAASKMMNVPVDKITVIDRVGSAPELLVLDGQVSPPNFSISHS
                     RNWVGCALSTEVMLGFDIELNDPARDIIALGDAAFQRNEQRWLTQQPDNSRIAAFYEL
                     WSTREALYKLMSNLGREMPSSSLNSAEKHDTSRGWHRRAFMHRGLTAVVCSDKSISKL
                     EKVDLAGLTPADLLGRA"
     gene            complement(3656..4246)
                     /locus_tag="VF182_11785"
     CDS             complement(3656..4246)
                     /locus_tag="VF182_11785"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6437805.1"
                     /translation="MTEYRLAMKEKLSSLIICVGLLAFGLIDATAEFQEEIPFVPTPV
                     EVVDKMLEMAKVRENDVLYDLGSGDGRVVIRAAKRYGARAVGIEMDQTLLDKARKSAE
                     TEGVGHLVEFRAEDALTTDLSPATVVTLYMLPWFNEAMKPNFRKFLKPGSRIVAHDFG
                     IEGWKPDKTEKLSEPEKKAGGYVHYHTIFLWNVRAD"
     gene            4581..5903
                     /locus_tag="VF182_11790"
     CDS             4581..5903
                     /locus_tag="VF182_11790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6437806.1"
                     /translation="MQNTTGPAARPPLSLPFYYGWFVLSIVFLTTLTSAGVRSSPSVL
                     IHPLEAEFGWSRALIASAVSMNLLLFGIAAPISGWLIDKYGPRKVMIGSLTLLIFGVS
                     GTMAMDQFWQFFLVWGVVVGLGAGGVGSVLTATVGNRWFVAKRGLALGILGSASSTGQ
                     IIFLPLFMAAITYAGWRMGSMALIIVAIILVPLIYFFMRDDPSEVGLEPYGAGDPAAT
                     SSGGMASLRGMRSGNATITAREVISHPTFWLLASSFFVCGGTANGLIGTHLIPHEIDR
                     GIPQITAASLLGVMGALNMVGTIFSGWMIDRVQPQRWLALVYALRGLSLLMLPFVQNI
                     SGFFLFAVIYGLDWFATVPPSMAITADTFGKQNVGKVYGWIFMSHQIGAAIMASSAGA
                     LRTWLGDYHYAFLSGGVIALVAAGLALQIKTKPRAVPPVTVHVQPAGA"
     gene            complement(5938..6345)
                     /locus_tag="VF182_11795"
     CDS             complement(5938..6345)
                     /locus_tag="VF182_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437807.1"
                     /translation="MKNISTSFYLFALIALLASSCGRDLGTAQGVTEAFVDHHYVSFD
                     LQKAKDYTVSIALDKVNEEIRLTTGQTIDASTQKPRVNYRLLEKKEGDRRASFLYEGT
                     IQSDDGTSFTRKWLIAARRDGNHWRVSNFSESD"
     gene            complement(6354..7109)
                     /locus_tag="VF182_11800"
     CDS             complement(6354..7109)
                     /locus_tag="VF182_11800"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6437808.1"
                     /translation="MLQEEISFSTSDGKTIVGILHHPAGESPRGAIILCHGMDSSKDS
                     EKLVSLGRGLATLGFLTLRFDFAYVGQSSEKFEDITYSGEVEDLAAACSFIRDRFSGG
                     IGILGSSMGGTVALLFAAQDPDIAALVTIAAPVHPEAFPRRILSPKGLQQWRDRGFTF
                     YNGQRLNLALLEDLEKINVVESARKIACPLLIIHGDTDEVVPVEEAHELNACISGKKK
                     LLILKGTDHRLSNPVTMHQAFTEAVDWLTAHVG"
     gene            7265..8173
                     /locus_tag="VF182_11805"
     CDS             7265..8173
                     /locus_tag="VF182_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX6437809.1"
                     /translation="MNDLIKRYTDYLRDERNLSAHTLRNYLSDLNQFEQFLRDSGLCL
                     DKNGNMDEQRLDIHVARAYLASINKDRKKTSVGRKLAALKGFFKYLAATGVIATNPLS
                     LIQTPKQEKPLPSFLSVDDVFQLLGGIVIKGSLDVRDRAMLEVFYSTGIRVSELVGLN
                     WGDVDFQLGIIRVVGKGSKERIVPIGAIAMQALQDYSLEHGKNWNLPCNGENPVFLNR
                     HGKRITTRSVERSVKKHLKACGIAVPIGPHGLRHTFATHLLNSGADLRVIQEMLGHAS
                     LSTTQHYTHLNLGQLTAVYDKAHPRA"
     gene            8266..8796
                     /gene="hslV"
                     /locus_tag="VF182_11810"
     CDS             8266..8796
                     /gene="hslV"
                     /locus_tag="VF182_11810"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX6437810.1"
                     /translation="MFEGTTILAIRHQGKVVVVGDGQVSLGQTVMKHSARKVRKLHHD
                     KVIAGFAGATADAFTLFEKFEAKLEQFNGNLKRAAVELAKDWRTDRILRRLEALLIIA
                     DINDLLVISGAGDVVEPDDGVIAIGSGGNFALAAARVLVKHTQLDARTIAEESMRVAA
                     SLCVYTNEQLTFEELS"
     gene            8970..10298
                     /gene="hslU"
                     /locus_tag="VF182_11815"
     CDS             8970..10298
                     /gene="hslU"
                     /locus_tag="VF182_11815"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX6437811.1"
                     /translation="MTPREIVSELDRYIVGQKDAKRAVAIALRNRWRRQQVSEDLREE
                     IVPKNIIMIGPTGVGKTEISRRLAKLAQAPFIKVEASKFTEVGYVGRDVESIIRDLTD
                     LAVNMVKEEEKQKVEIKAHEVAEERVLDLLLPGAGGGTDGEVIDSARVQGTREKLKKM
                     LREGKMDERFVDIEVTQSAMPMIEVLTPQGMEGMEFNLKEMFSNLMPKKTKKKTVKIP
                     EALEILTQEEAGKLVDMDKVSGEAVRRVEQSGIVFLDEIDKIAGREAGQGPDVSRQGV
                     QRDLLPIVEGSTVNTKYGMVKTDHILFVASGAFHTAKPSDLIPEFQGRFPIRVELSSL
                     GKDDFVRILTEPKNALIKQYEALLETEGVHLRFGAAAIEEIARIASDVNDRMENIGAR
                     RLHTILERLLDDVSFAAPEMHGKEVAIDAEYVRERLTPILKNEDLSRYIL"
     gene            10313..11197
                     /gene="argB"
                     /locus_tag="VF182_11820"
     CDS             10313..11197
                     /gene="argB"
                     /locus_tag="VF182_11820"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX6437812.1"
                     /translation="MDDFIGKAEVLMEALPYIQRFYGKTFVVKYGGHAMENEELKHSF
                     AQDIVLLKYVGINPVVVHGGGPQIDTVLDKMGITSRYVRGMRVTDQATLDIVEMVLVG
                     KVNKEIVNLINRHGGMAVGLSGKDGGLILARKMNVTVPSANGAPPELIDIGMVGEIIG
                     INPLVIESLDQNKFIPVIAPVGVGQEGETYNINADLVAGEVAEALHAEKLIMMTDVEG
                     VKDKRGSLLSTLKENQARKMIQDGVVGSGMIPKVECCVNALKGGVGKTHIVDGRVKHA
                     VLLEIFTKEGIGTEVVRK"
     gene            11198..12385
                     /locus_tag="VF182_11825"
     CDS             11198..12385
                     /locus_tag="VF182_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX6437813.1"
                     /translation="MNNLEIAALTDKYVARTYARIPIALVRGKGTKVWDADGKEYLDF
                     LAGIAVNNLGHCHPAVVRAIREQSKKLLHVSNLYHILPQSELARELCHHSFADRAFFC
                     NSGAEANEAAIKLVRRYGLEKLGGRHEILSTHNSFHGRTLATLTATGQEKIRAGYDPL
                     PSGFRQVPYNDLAAMEEAIDEKKTAAILVEPIQAEGGVNVPDDAYMRGLRELCDQRGI
                     LLVFDEVQTGMGRTGTLFGYEHFAIQPDIMTLAKALGGGLPLGAMLAREEIAASFGPG
                     SHASTFGGNPVACSAGLMVMQTLLASALDNCRQMGNYFFRGLEALQKRFSFIREVRGK
                     GLMIGMELEMEGSKIADSCMQEGLLLNCTAYKVLRFVPPLTIKKKEIDRGLDILEKVL
                     ARQ"
     gene            12407..13408
                     /gene="argF"
                     /locus_tag="VF182_11830"
     CDS             12407..13408
                     /gene="argF"
                     /locus_tag="VF182_11830"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX6437814.1"
                     /translation="MNKRDLLNFADWSRDELESILRLARDLKQKQKQGVMHHLLEGKG
                     LGMLFEKPSLRTRVTFEAGMVQLGGHAVFLSPTEVQMGVRETPEDCARSLSRWVDMIV
                     VRTFAQATLEEMARAASVPVINALTDLYHPCQVLADCLTLLEHKGKLQGLKVAFVGDG
                     NNMVHSWMEAAEKIPFSFALACPKGYEPDRDIAARVKRNGARVTVTHSIRDALNGADA
                     IYTDVWTSMGQEWDVATRLKAFHEYQVNHRVLAMAKKDAVVMHCLPAHRGQEITDEVL
                     ESPQCIAFDQAENRLHAQKAVMVWLLKGARSSTVPKSKGSSKKANQSTGSRGSNKKR"
     gene            13405..14613
                     /locus_tag="VF182_11835"
     CDS             13405..14613
                     /locus_tag="VF182_11835"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX6437815.1"
                     /translation="MNVKKVVLAYSGGLDTSVILRWIKEEYGCEVIAFCADLGQGEDL
                     QAIKRKAIDTGASKAVIGDLREEFVKNYVFPMLRGNAIYEGSYLLGTSIARPLIAKAQ
                     IDIAVKERADAVSHGATGKGNDQVRFELTYYALKPAIKVIAPWRLWRLNSRTSLIAYA
                     RKHGIPVPVTKARPYSTDRNLFHISYEGGILEDPWKEPPPDMFVWCRPLERAPNRSEY
                     VEIDYLRGDPIAINGKTLSPAKLLAQVNDLGAKHGIGRVDLVENRYVGMKSRGVYETP
                     GGTILHAAHRALESITMDREVMRLRDSLIPRYAEMIYYGYWFAPEREVLQGAIDAAQR
                     NVNGTARLKLYKGNVVVAGRKSGSSLYDPRVATFEQDTVYQQADAEGFIRLNALRLRL
                     QKLSKIKRSY"
     gene            14610..15998
                     /gene="argH"
                     /locus_tag="VF182_11840"
     CDS             14610..15998
                     /gene="argH"
                     /locus_tag="VF182_11840"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX6437816.1"
                     /translation="MKVSKARQKKLWGGRFASATAASVEAFTASIHIDSRLYRYDIMG
                     SIAHTKMLSKQGIIAAGDGRRIVRGLKQIEAEIEQGNFNFSSADEDIHMNIERRLTEK
                     IGSAGGMLHTARSRNDQVALDMRLFLREEVRSIIDDLSALQREIAGAAKRHIDVIMPG
                     YTHLQRAQPVLFAHHLLAYYDMFDRDRQRFTESLQRINVLPLGAGALAGTTFPIDRAY
                     VASLLGFPEISKNSIDAVSDRDFLLEFTAGSSILFVHLSRLADEWVLWSSQEFSFIEL
                     PDGYCTGSSMMPQKKNPDVLELIRGKTGRVFGHLQALLTIMKGLPLAYNRDLQEDKIP
                     LFDTVDTVKASLQMMREIVRGVKVKANRMLAAAQDGFMNATDLADYLVDRGEPFRSAH
                     EIAGRIVQHCIAKGRRIEQLPLEELRDFSEKIEKDVHRYLSIEGVVGRRQALGGTARN
                     NVLRRLKEIRSA"
     gene            15995..16435
                     /locus_tag="VF182_11845"
     CDS             15995..16435
                     /locus_tag="VF182_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437817.1"
                     /translation="MKSFLVFASLILLLCIGCGKKGEPRAPELAMPQLIKNLTAKPDR
                     TGIVLTWSRPTEYVDGRSLTDLTGFVIFRKDLSPTCPECPVPYRPLAAVNVEDREKFV
                     KQKQYRFIDENAQAQMIYRYRVSSQLKDGSLSNPSNEVEATRGP"
     gene            16435..17706
                     /gene="lysA"
                     /locus_tag="VF182_11850"
     CDS             16435..17706
                     /gene="lysA"
                     /locus_tag="VF182_11850"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX6437818.1"
                     /translation="MNHFEYRNGEMYAEGVAVKRIAREVGTPAYIYSLATLKRHYQVF
                     DQAFAKVPHIVCFSVKANSNLAVLRTFAKEGSGFDIVSAGELFRALKAGGDPKKIVFS
                     GVGKKKDEIEYALSSEILMFNVESEDELVALNEIAGAIGKKAPISIRVNPDVDPQTHP
                     YISTGMKKAKFGVEIKKSLDTYKKAAALPNVDVVGVDCHIGSQLTSLSPFVDALTRVR
                     EYLDRVLVGSLEKEGAQIRYLDLGGGLGINYNDETPPQPEEYAKAIIEGLGGLDVTLI
                     LEPGRVIVGNAGILVTEVQYVKDNESKKFVIVDGGMNDLIRPALYGSYQAIRPVVETR
                     AEKIFADVVGPICESGDFFAKDREITRPQRGDLLAVMSAGAYGFTMASNYNSHPKAPE
                     VLVDGDKYYVVRKRESFEDLINGETIPAALE"
     gene            17915..18730
                     /gene="dapF"
                     /locus_tag="VF182_11855"
     CDS             17915..18730
                     /gene="dapF"
                     /locus_tag="VF182_11855"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX6437819.1"
                     /translation="MPFTKMHGCGNDFIFIDCLKNDVPDLGAISGRLCDRRFGIGADQ
                     LLTVHPSKIADFKMEIYNADGSQVEMCGNGIRCFAKYVYEHGLTRNKELEVETLAGII
                     RPRIVGDLVEVDMGEPTLEGRKIPVDDDGQIVNRPLIVDGRTYTVTCVSMGNPHCVTY
                     FDDIESLDLEKVGPGFEHHAFFPKRVNTEFIKVLSPNEVNMRVWERGAGETWACGTGA
                     SAVAVAGVLGGKTERKLTVHLKGGDLFIEWRDNNRVYMTGAAEEVFQGRVKIS"
     gene            18740..19648
                     /gene="dapA"
                     /locus_tag="VF182_11860"
     CDS             18740..19648
                     /gene="dapA"
                     /locus_tag="VF182_11860"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX6437820.1"
                     /translation="MFTGSMVALITPFTGGKVDWQSLERLIDFHIESGTHGIVPCGTT
                     GESATLSHEEHDEVIKAVIKAVNRQLPVIAGTGSNSTDEAVRLTRAAEKSGADGALMI
                     SPYYNRPTQEGIYQHYKKVASEVGIPIIIYNIPGRTGSKIEPETLARLAEIDNIAGVK
                     EATGSVDQAIDVIRLCGDNLALYSGEDTLTFSLMSLGGKGVISTVANVAPREMSQLTE
                     ACLKGEWERGRDLQLKLTPLIRSLFVETNPIPVKTAAALMGKCSGELRLPLTPMAEGN
                     LQKLKQAMIEFGLVKQSHHIAKANES"
     gene            19693..20505
                     /gene="dapB"
                     /locus_tag="VF182_11865"
     CDS             19693..20505
                     /gene="dapB"
                     /locus_tag="VF182_11865"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX6437821.1"
                     /translation="MTAKNLNLIVCGVGGRMGGAVVRAIQQTAGVALIAGIDKSGGTR
                     LGKDAGEISAAGNFGISVTDRIEPYLKPGTVIIDFTRPEASLTYLRAAAKKAVPIVIA
                     TTGFDPRQIAEIKRLSRQTPALLSANTSLGVNVLVNLLGKAAKMLGDDYDVEIVEAHH
                     RFKKDAPSGTALALGRAVAGALSRDLDKVGIHGRKGIIGERSKKEIALLSVRAGDIVG
                     EHTVIFGGIGERLEFIHRAHNRDTFAHGAIRAAQWLTQQKPGLYGMQDVLGL"
BASE COUNT         4741 a         5332 c         5954 g         4620 t
ORIGIN      
        1 atgagatgag ttggccggat attaggtcat tgattccgca cagcggcgcg atggtgctac
       61 tcgatcgggt cattgcgatc gatgaagaga ctctgtgcgc ggaggtgctt atcgggcctg
      121 atagtttgtt cggttcggcg gaaggtgtcg gcgcatgggt gggtctcgaa tacatggcac
      181 aaaccatcgc tgctttcgcc ggatataccg catccttgcg cggcgaagcc gtcaaaccag
      241 gatttttact cggagcgaga cgctatgagt gtaccctccc ggtattcagt gtgggcagct
      301 tactgaaagt ccatgtccgc cgcgtgttgc agagcgaaaa cggtttagga tcgtttcagt
      361 gccgtatcga gcatggcgaa gaggaagtgg ctactgccat gctgacggtt ttccaacctg
      421 ccgatgcccg agattttgtc aaaggggact cgaatgaata agaccgtcct ggtaaccggc
      481 tcgagccgcg gcattggcag agccatcgcg tatcggctcg cgcaggacgg ttacgacatc
      541 gtggcgcatt gccgcgtgcg ccgccaagag gccgatgaat taggccgatc cattgttgca
      601 ctaggccggc aagcgcgcgt gttgcagttc gatattggcg atcgcgccgc cgccgcggca
      661 acactggaag ccgacgttga gcggcatggc tgttactacg gcatagtatg taacgccgga
      721 gttgcccgtg acagcgtttt tcctggcatg tcaggcgagg attgggacgt tgttctcaga
      781 acaaatctag acggctttta taatgtgctc aatcccttga tcatgccgtt ggtgcggcga
      841 cgagcgccgg ggcggattgt cactctggcg tcggtgtctg ggttgatcgg caatcgcggt
      901 caagtcaatt acagcgcagc gaaggcgggc atcattggcg cgaccaaagc attggcgctg
      961 gagctggcaa cgcgcgagat caccgtaaat tgcgtcgctc ccgggctgat tgagacagaa
     1021 atgactggcc aagcgcctgt agatgaaata ctcaaactga ttcctgcagg tcgaaccggt
     1081 actcccgacg aggtcgcggc agcagtgagt ttcctgctta gcgatggcgc ggcgtacatc
     1141 actcgccaag tcatgtcggt caatggcggg ctcatatgag gcgtcgggtg gtggtcaccg
     1201 gcatggcggg catcagcccc atcggcaacc actggacggc tattcgggcg cgcttggaga
     1261 gctaccgcaa tgccgtcatg cgcatgccgg agtggtccga ctatgatggg ctgaatactt
     1321 tgctaggcgc gccggcggca ccgtttgagc tgtctgaacg ttacaccagc aaggcaaccc
     1381 gcagcatggg gagagtagcg cttatggcaa cccgcgccag cgaactagct ttactcgatg
     1441 ccgggctgct cgatgacccg cttttgaaaa gcggtcactt gggcatcgcc tatggctctt
     1501 cggccggcac gcccaaagct atttgcgatt tcggcaagat gatcgatgaa aaaagtacca
     1561 agggcatcaa tgcgacgact tacataaaaa tgatggggca taccgcgccg gtaaatctcg
     1621 gggttttttt gggcattacg ggccggattg tcacaacctc cagcgcttgt acctcgggta
     1681 gccaagggat cgggtacgca tatgaaacca ttcgcagcgg gcgccagcga gcgatgattg
     1741 ccggcggcgc cgaagaactg tgcgtcaccg aggtagcagt tttcgatact ctatttgcca
     1801 ccagcgcgcg caacgacgcc gcccactcga cacctcgtcc tttcgatgca gagcgcgatg
     1861 gccttgtcct gggcgaaggc gctggcagct tggttttgga aaatctcgaa catgcccgcg
     1921 cccgcggcgc taaaatcttt gccgaagtcg tcggcttcgg caccaacagc gatggcagtc
     1981 acatcaccca gccaaatgcg cgcaccatgc aaattgcgat ggagttgtcg cttgccgatg
     2041 ccgacctttc accgtcggct atcggttaca tcaacgctca cggaaccgcc acccagcagg
     2101 gcgatgtcgc ggagtcgcaa gctacacggc aggtcttcgg cggcaacgtg ccgatcagtt
     2161 ctctcaaaag ctatatggga cacacccttg gcgcttgcgg cgctttggaa tcgtggatca
     2221 gcatcgagat gatgcgcgca gcgtggtttg ccccgacaat taatcttgcc cagatcgatc
     2281 cgcactgcgc cgatctcgac tatatcgtcg gcgccgggcg gcagctcgaa tgcgaatacg
     2341 tcatgtccaa caacttcgcc ttcggcggca tcaatacttc attaatcttc aaaaggtacg
     2401 attaagcgaa agtggctctc acggtgcgtc acagcagaag ctggactcaa acctagataa
     2461 atcgatcaaa gtcgaaggag aaccgaatgg caaatgtggt atcgatggca attttgacgc
     2521 tgtgcctggc aattggtttg cccgcgcatg cccgggacac gagacatgtc ctgtcgattg
     2581 cggttgcgat ggagaccaaa gatgcgctgg acaagcttga cggatcggtt agattttatt
     2641 tcggggatca atcggcgccg caggtcctag cgatgctagg cagcgatgtc tcgaatcgca
     2701 agacgaacgc cttcgggaaa tccgacgaga aagcctgcaa ctgggcattc ctgtcggcaa
     2761 tgttagctct tgaaaagcgc gcgcatcaga tgggcgcaaa cgccgttatc aacatcgtca
     2821 gttattacaa caaaaaagtg atgtcgagcg cgaccgagtt tgaatgccat gcgggcggga
     2881 tcatggctgg cgtggcgttg aaaggtgatt tcgtaaaaat aaccgatgga tgagcgcaac
     2941 agccgcgctg caactgtctg gttgctcgac attagacggg tgcctgagtg cgttgtcaat
     3001 tatttcgtgc aacgactcgc ggcctctgag ttccaccgat acgccaagtt tatgcggagc
     3061 cagcgaaagc ggcagttcct gcttgcgcgg atgcttcttc gtctagcggc gtccaagatg
     3121 atgaacgtgc cggttgataa aatcacggtc atagaccggg tgggatctgc tccagagctt
     3181 ttggttctcg atggacaggt ctcaccgcca aatttcagca tctctcacag tcggaattgg
     3241 gtcggctgcg cgctcagcac cgaggtgatg ctgggcttcg atatagaact caacgatccg
     3301 gctcgagata tcatcgctct tggcgacgcg gcatttcaac gcaatgagca gcggtggctg
     3361 acgcagcaac cagacaactc tcggattgcg gcgttttatg agctgtggag cacccgggaa
     3421 gctttgtaca agctgatgtc taacctggga cgggagatgc cgtcctcatc cttgaacagc
     3481 gcggagaagc acgatacttc acgaggctgg catcgccgtg ccttcatgca tcgagggctc
     3541 acggccgtgg tttgtagcga taagtcgata tcgaagttag agaaggtcga tcttgccggg
     3601 ctcaccccgg cagacttgct gggccgcgcc taacctttgt tgcgaatcgc gacgattagt
     3661 cggcgcggac gttccataag aaaatggtgt ggtagtgaac gtagccgccg gctttcttct
     3721 caggttcgga taatttttcc gtcttatcag gtttccaacc ttcaatgccg aagtcgtgag
     3781 cgacaatacg ggatcccggt ttaaggaatt tccggaaatt gggcttcata gcttcattga
     3841 accagggaag catgtaaagc gttacgactg ttgccggtga gaggtcggtt gtcaaagcat
     3901 cttctgcgcg aaactcgacc aggtgaccga cgccttccgt ctcggcggat ttgcgcgctt
     3961 tatcgaggag cgtctggtcc atctcgatgc cgaccgcccg cgctccgtac ctcttggccg
     4021 cgcgaattac gactcgtcca tcaccgctcc caaggtcata cagcacatca ttttcccgga
     4081 cttttgccat ttcgagcatt ttgtcgacga cttcgacggg tgtcgggaca aaagggattt
     4141 cttcttgaaa ttctgcagtc gcatcaatga ggccgaaggc gagtagccca acgcagataa
     4201 tcagcgagga taatttttct ttcatcgcga gcctatactc agtcattcag cattgccggt
     4261 gatccctggc gcggcaggtc ttcttggcac ggttcgtcgg tatagttgaa atccgtgtac
     4321 ggctatcttt ggttcgcatc tcaggacggg gcttgtcatc agtggcccgg aactattcaa
     4381 ccgattaacg acgaaatatc aagcatatct gagcccttcc ctttggggcc ccgaaggatc
     4441 ccggagctac agcggccgca aagccgcgtt tgtcttcaaa cctaaggcgc tgctgcttga
     4501 tcactcgcga ttagatcttg tccctgcgcg caggcacaag cgagtttgtg aagtaaccca
     4561 aaactggttt atataaaaag atgcagaaca ctacaggtcc cgcagcgcgc ccgccgctat
     4621 cgttgccctt ttattatggc tggttcgtct tgtcgattgt ctttttgaca acattgacta
     4681 gtgctggagt tcgttcctcg ccgtcggtcc tgattcaccc tttagaagct gagtttggct
     4741 ggagccgagc cctaatcgcc tcggccgtca gtatgaatct gttgttgttt ggcattgccg
     4801 cgccgatcag cggttggctc attgataaat acgggccgcg caaagtcatg atcggcagcc
     4861 tgacgttgtt gatttttggc gtaagcggca ccatggcaat ggatcagttc tggcaatttt
     4921 ttctggtttg gggagtggtc gtgggacttg gcgccggcgg ggtaggttcg gttctgaccg
     4981 cgaccgtggg gaaccgctgg ttcgtcgcga aacgaggact cgcattgggc attctcggca
     5041 gcgccagctc cacgggacaa ataattttcc tgccgttgtt catggccgcc atcacctatg
     5101 ccggctggcg catgggctcg atggcattga tcattgtcgc gattatcctg gtgccgctaa
     5161 tttacttttt catgcgcgac gacccgtccg aagtcggact ggaaccttac ggggcgggag
     5221 acccggcggc taccagctca ggcggcatgg cttcgctacg gggtatgcga tctggcaacg
     5281 caaccattac ggcgcgcgaa gtgatttccc atccaacctt ttggctgctg gcgagtagct
     5341 tctttgtctg tggcggcacc gccaacggtc tgatcggcac tcatttgatt ccacacgaga
     5401 tcgaccgggg tattccacag atcaccgccg cgtctttact tggcgttatg ggcgcgctca
     5461 acatggtggg cacgattttt tccggttgga tgatcgaccg tgtccagccg cagcgatggc
     5521 tggcgttggt ctatgcactt cgcggactct cgctgctcat gctgcccttt gttcagaata
     5581 tctcgggatt ttttctgttc gcggtaattt acggcctgga ttggttcgct acggtgcccc
     5641 catcgatggc gatcactgca gacacgttcg gcaagcaaaa cgtcggtaag gtttatggct
     5701 ggatttttat gtcgcatcag atcggcgcgg cgattatggc gtcgagcgcg ggagcccttc
     5761 gcacctggtt gggcgactat cattatgcat ttctttcagg cggggttatt gcgctggtcg
     5821 cggcgggact ggcattacag ataaaaacca agccgagagc cgttccgccg gttacagtgc
     5881 acgtgcagcc tgcaggagcc tgagcagagg tgaacggccg aaggcccagt gtcgccttca
     5941 gtcggattcg ctaaagttcg acacgcgcca gtggttgccg tctcggcgcg cagctatcaa
     6001 ccacttgcgg gtaaaagatg tgccgtcgtc ggattgaatc gtcccttcgt aaagaaaaga
     6061 agcccgccga tctccttcct ttttctccag taaacggtag ttgactcgag gcttctgagt
     6121 cgaggcatcg attgtctgcc cggtggtcag cctgatctcc tcgttaactt tgtccaaagc
     6181 aatactgacg gtgtaatctt tggccttctg caaatcgaaa ctgacgtagt gatgatcgac
     6241 gaatgcttcg gttacgccct gtgcagtacc gagatcgcga ccgcagctac tggccaacag
     6301 agcgatcaga gcaaacagat agaaagatgt ggagatgttt ttcatttggc gcgtcaacca
     6361 acgtgtgccg taagccaatc gacggcttcg gtaaaagctt gatgcatggt caccggattg
     6421 gacaggcgat ggtccgttcc tttcaatatc aacaattttt tcttacccga aatgcacgcg
     6481 tttaactcat gcgcttcttc gaccggcacc acctcgtcgg tgtcgccatg gataatcagc
     6541 agcggacaag cgatcttgcg cgcggattcg accacgttga tcttttctaa atcctcgagc
     6601 aacgccagat taagccgttg cccattgtag aaggtaaagc cgcggtcgcg ccattgttgc
     6661 agccctttgg ggctgaggat ccgccgcgga aaagcttctg gatggacggg cgcggcaatc
     6721 gtaaccagag ccgcaatatc cggatcttgg gcagcaaaca gcaaagccac cgtcccgccc
     6781 atgctggagc cgagaattcc gattccgcct gaaaaacgat cgcggatgaa cgaacatgcc
     6841 gcggccaagt cctcgacttc gccgctgtaa gtgatgtctt caaacttctc gctggattgg
     6901 ccgacatatg cgaaatcaaa tcgcagcgtg agaaatccta gcgtagctaa accccggccg
     6961 agggaaacga gcttttcact gtctttgctg gagtccatgc cgtggcagag gatgatggct
     7021 ccccgcggcg attcgccggc tggatggtgc agtatgccga cgatagtctt accgtcgctc
     7081 gtactaaaag atatttcttc ttgcaacatt ggctcggcgc cggttagcag gactctagct
     7141 cagatttagc taagtttgaa gtcggtgagg ccggcattgc ttctgtcatg gcagaaacct
     7201 tgaagtcgtt gatttcgttt gctgaggggg catgttatcc tgtgaaagtt cggcaacccg
     7261 caaagtgaac gaccttatta agagatacac cgattatctc cgcgatgagc gcaacctctc
     7321 ggcgcacaca ctgagaaact atttaagcga tctaaatcag ttcgaacagt ttcttcgcga
     7381 tagcggcttg tgtctcgata aaaacggcaa catggacgag caaagactgg acatccatgt
     7441 ggcgcgcgct tatctcgctt caatcaataa ggatcgaaaa aagacttctg tcgggcgcaa
     7501 gctggccgcg ctgaaaggat tttttaaata tttggcggcc acgggggtga tcgcgacaaa
     7561 cccgctatca ctgattcaaa cgcccaagca ggaaaagccg ctgccatctt ttctctcagt
     7621 ggatgatgtg tttcagctgc tcggcggtat tgtcatcaaa ggatccttag atgtgcgcga
     7681 ccgagccatg ctcgaagtgt tttattcgac gggaatacgg gtgagtgaat tggtggggct
     7741 taactggggt gatgttgatt tccagctggg gatcatccga gtggtcggaa aaggctccaa
     7801 agagcgcatc gtgccgattg gcgcgatagc aatgcaagcg ctacaagact atagtttaga
     7861 gcatggaaag aactggaatt taccctgcaa cggcgaaaat ccggtctttc ttaaccgaca
     7921 tggcaaaagg ataacgacac gaagcgtaga acgcagcgta aaaaagcatt tgaaggcttg
     7981 cggaattgct gtgcctatcg gtccacatgg tttacggcac acgtttgcga ctcaccttct
     8041 aaatagcggc gccgatcttc gagtgataca ggaaatgctt gggcatgcga gtctttccac
     8101 gactcaacac tatacgcacc tgaacctggg tcagttaacg gcggtatacg acaaagctca
     8161 tccacgggcc tgaaagcagg gcttaagcga tacgcgcgag gcaccgcgag attatcttcg
     8221 agtcgtggat cattgaaatt tgaatgatga atggtgagtt gagcgatgtt tgaaggaacg
     8281 acaatattag cgattcgaca ccagggaaaa gtggtggtcg tcggcgacgg gcaggtgagt
     8341 ctcggtcaaa ccgtgatgaa gcacagcgcc cgcaaggtcc gcaagcttca tcacgacaaa
     8401 gtcatcgccg gttttgccgg cgccactgcc gacgcattta cgttgttcga gaagtttgaa
     8461 gccaagctgg aacagttcaa cggcaatctt aagcgcgcgg cagtggagct ggccaaggac
     8521 tggcgcacag accgaatctt gcgtcggctt gaggcgctac tgatcatcgc cgacatcaat
     8581 gatctgttgg taatttccgg agccggcgat gtggtagaac ccgacgatgg cgtcattgcc
     8641 atcggatcgg gaggaaattt tgcattggca gcagctcggg tgttggtgaa gcacactcag
     8701 cttgatgcgc gcacgattgc tgaagagtcg atgcgcgtgg ccgccagtct ctgcgtctat
     8761 actaacgagc aacttacctt tgaagagtta tcgtagcatg tcccgcttcg caccgagtcg
     8821 ggacggcgat tgacgcaaga aaccgtacat ttctgcaaca aaagagtatt tgcttttgtc
     8881 tttgaagttg ctaggagtta gctattctaa tgaagcatga acccattata tctttgcccg
     8941 attcgtctga ctctcccccg gctccggtga tgacaccccg ggaaatcgtt tctgagctcg
     9001 atcgctacat tgttggacaa aaagatgcca aacgcgcggt ggcgattgca ctgcgcaatc
     9061 gttggcggcg ccagcaggtt tcagaagatc tgcgcgaaga gatcgtacct aagaatatca
     9121 tcatgatcgg ccccacgggt gtcggtaaga ccgaaatctc acgccgtctt gccaagctcg
     9181 ctcaggcgcc attcatcaaa gtcgaagctt ccaaattcac tgaagtcggc tatgttggcc
     9241 gcgatgtcga atcgatcatc cgcgatctca ccgatcttgc ggttaacatg gtgaaagaag
     9301 aagagaagca gaaggtcgaa attaaagccc atgaggttgc cgaagagcgc gttttggatt
     9361 tgctactgcc cggggcgggc gggggcacgg atggtgaggt aatcgatagc gcccgagtgc
     9421 agggcacacg cgagaagctg aagaagatgc ttcgtgaagg caagatggat gagcgcttcg
     9481 tcgacattga agtcacccag tcggcgatgc cgatgattga agtgttgacg cctcaaggca
     9541 tggaaggcat ggaattcaac ctgaaagaaa tgttttccaa cttgatgccg aaaaaaacca
     9601 agaagaaaac cgtcaagatc cctgaagcct tggaaatcct tactcaggaa gaagccggaa
     9661 aattggtcga tatggacaag gtgagcggcg aagccgtgcg ccgggtggag cagtccggga
     9721 ttgtcttcct cgatgaaatc gataagattg ccggtcgcga agccggccag ggtccggacg
     9781 tttcccgcca aggcgtgcaa cgtgatttgc tgccgatcgt tgaaggttcg acagttaata
     9841 ccaaatatgg catggtcaag accgatcaca ttttgttcgt tgcttcagga gcgtttcata
     9901 ccgcaaagcc ttctgatttg atccccgaat ttcaaggtcg ctttcccatt cgtgtggagc
     9961 ttagttcctt gggtaaggac gacttcgtac ggatcctcac cgaacccaaa aacgcgctga
    10021 tcaagcaata cgaagccctg ctcgaaacag agggagttca cttaaggttc ggcgccgcgg
    10081 cgatcgaaga aattgccagg atcgcttccg acgtgaacga ccgaatggaa aatatcggcg
    10141 cccgccgcct ccacaccatt ctcgaacgtt tgctcgacga tgtctctttc gccgcgcccg
    10201 aaatgcacgg caaggaagtt gccatcgacg ccgaatatgt gcgcgaacga ctcacgccaa
    10261 tcttgaagaa cgaagatcta tcgcgctata tcctctgatc caaggcaggc gcatggacga
    10321 tttcatcggc aaggcagagg tgttgatgga ggcgctgccg tacattcagc gcttctacgg
    10381 caaaacgttt gtcgttaaat acggtggcca cgcgatggaa aatgaggagc tgaaacacag
    10441 cttcgcgcaa gacatcgtcc tgctgaagta cgttggcatc aatcccgtgg tagtgcacgg
    10501 cggcggcccc caaatcgata cggtgctcga taaaatgggg attaccagcc gctatgtgcg
    10561 cggcatgcgt gtcacggatc aagcaacttt ggatattgtc gaaatggttc tggttggcaa
    10621 ggtgaataaa gaaatcgtca accttatcaa tcggcatggc ggcatggccg tgggtttgag
    10681 cggcaaagac ggcggtttga ttctggcgcg caagatgaat gttaccgtcc cctcggccaa
    10741 cggggcgccg ccggaactca tcgatatcgg catggtgggc gagatcatcg gcatcaatcc
    10801 tttagtgatc gagtcgttgg atcagaacaa gtttattccg gtcatcgccc ctgtcggcgt
    10861 cgggcaggag ggcgagacct acaatatcaa cgccgatctg gtggccggtg aagttgccga
    10921 agcccttcat gccgaaaagc tcattatgat gaccgatgtc gaaggagtga aagacaaacg
    10981 ggggagctta ctgagcacgc tcaaggagaa tcaagcgcgc aagatgattc aggacggggt
    11041 ggtcggctcc ggaatgatcc ctaaggtcga atgttgcgtc aatgcgctca agggcggagt
    11101 cggtaagacc catatcgttg atggtcgggt aaaacacgcg gtactcctgg aaatcttcac
    11161 caaagagggg atagggactg aggtggtgcg taaataaatg aacaacttgg agattgctgc
    11221 gctaactgac aaatatgttg ctcgcaccta cgcccggatt ccgatcgcct tggttagagg
    11281 caaagggacg aaagtctggg atgccgacgg taaagagtac ctcgatttcc tggccggaat
    11341 cgctgtcaac aatttgggtc actgtcatcc ggctgtcgtc cgcgcgatcc gggagcaatc
    11401 gaaaaaactg ctgcacgttt cgaatctcta tcacatcctg ccgcagtcgg agttggcgcg
    11461 cgagttgtgc caccattcgt tcgccgatcg ggcttttttt tgcaacagcg gcgccgaggc
    11521 aaacgaggcg gcgatcaagc tggtgcgccg ctacggcctg gagaagctcg gcggcaggca
    11581 cgagattctt tctacacaca actctttcca cggccgcaca ctcgcaaccc tgaccgccac
    11641 cgggcaggag aaaattcgcg caggttacga tcctttgccc agcgggtttc gccaagtgcc
    11701 ctacaacgat ttggctgcca tggaagaagc catcgatgag aagaaaaccg ccgctatctt
    11761 agtcgagccg attcaggctg aaggcggcgt caacgtgcca gatgacgcgt acatgcgagg
    11821 gctccgtgag ctctgcgacc agcgcggcat cttgctcgtc ttcgatgagg ttcaaaccgg
    11881 catggggcgc accggcacgc tgttcggcta cgagcatttc gcaatccaac cggacataat
    11941 gacgttggcc aaggccctcg ggggaggctt gccgctgggc gcgatgttgg cacgcgaaga
    12001 gatcgccgcc agtttcggac cgggtagcca tgcatcgaca tttggcggca atccggtggc
    12061 atgcagcgcc ggcttgatgg tgatgcaaac tctattggcc agcgcgctag ataactgccg
    12121 gcaaatggga aattattttt ttcggggcct ggaagctttg caaaagcgct tttcattcat
    12181 acgggaagta cgcggcaaag ggctgatgat cggcatggag ctggagatgg aaggcagcaa
    12241 aatcgccgac agctgcatgc aagagggatt gctccttaac tgcaccgcct acaaagtcct
    12301 ccgtttcgtt ccaccgctga cgatcaagaa aaaagagatt gaccgcggcc tcgacattct
    12361 cgaaaaggtt ctggcccgcc aataaccgtt gggcggtcat cttgtcatga ataaacgaga
    12421 tttgttgaat ttcgctgatt ggagccggga tgagctcgaa tcgattctga gactggctcg
    12481 cgatctaaaa caaaagcaga aacaaggcgt catgcatcac ctgctcgagg gcaaaggcct
    12541 gggcatgctg tttgagaaac ccagtttgag aacgcgcgtg acttttgagg ccgggatggt
    12601 tcagctcggc ggccatgccg tatttctctc gcccacggaa gtacagatgg gcgtgcgcga
    12661 aactccagag gattgcgcgc gcagcttgag ccgctgggtc gatatgattg tcgtgcgcac
    12721 attcgcccag gcgactctgg aagaaatggc tcgcgctgcc tcggtgccgg tgattaacgc
    12781 attgaccgac ctttatcatc cttgccaggt tcttgccgac tgcctgacat tgttagaaca
    12841 caagggcaag ctacaaggat taaaggttgc gttcgttggc gacggtaaca atatggtgca
    12901 ttcctggatg gaggctgccg agaagatccc attttcgttc gcattggcat gtcccaaggg
    12961 ctacgaaccc gacagagaca tcgcggctcg ggtaaaaaga aacggcgcgc gggtgaccgt
    13021 aacccattcg atcagagatg ctttaaacgg agccgatgcg atctacaccg atgtctggac
    13081 cagcatggga caggagtggg acgtcgccac tcgtctcaaa gctttccacg aatatcaggt
    13141 aaaccatcga gtcctggcga tggccaagaa agatgctgtg gtgatgcatt gtttaccggc
    13201 ccatcggggc caggaaatca ccgacgaggt tttagagagc ccgcagtgca ttgccttcga
    13261 tcaggctgaa aacagactgc atgcgcaaaa agccgtcatg gtctggttgc tcaagggcgc
    13321 gcgatcttca acggttccaa agtccaaagg ttcaagtaaa aaagcaaatc aatctacggg
    13381 aagcagaggt tccaacaaga aaagatgaac gtcaagaagg tggtgctggc ttactcggga
    13441 ggattggaca catcggttat tctgcgttgg attaaagaag aatatggctg cgaagtcatc
    13501 gctttctgcg ccgaccttgg acagggtgaa gacctgcagg cgatcaaacg caaggcgatc
    13561 gacaccggcg caagcaaagc ggtgatcggc gacctgcggg aagagttcgt caaaaattat
    13621 gtcttcccga tgctgcgtgg caacgcgatc tacgaaggtt cctacctgct cggcacttcc
    13681 atcgcccggc cgctcatcgc caaggcgcaa atcgacatcg ccgtgaagga gcgcgccgat
    13741 gcggtgtcgc acggcgccac tggtaagggc aacgaccagg tgcgttttga attgacctat
    13801 tatgcgctca agccggcgat caaggtgatt gcgccgtggc gcctttggcg tctcaattca
    13861 cgcacctctt tgatcgccta tgcgcgcaag cacggcattc cggtgccggt gaccaaggcg
    13921 cggccctaca gcaccgatcg aaatctcttt catatcagtt acgagggcgg cattctcgag
    13981 gatccatgga aagagccgcc gccggacatg tttgtctggt gccggccgtt ggaaagggcg
    14041 cccaatcgat cggagtatgt tgaaatcgat tatttgcgtg gcgatccgat cgcgatcaat
    14101 ggcaagacat tgtccccggc caagcttctg gcgcaggtga acgatcttgg cgccaagcat
    14161 ggcatcggcc gcgtcgatct ggtggagaat cgctatgtcg gcatgaaatc acggggtgtt
    14221 tacgagactc ctggcgggac catattgcat gcggctcatc gcgcgctgga gtcgatcacc
    14281 atggatcgcg aggtgatgcg gctaagagat tcgttgattc cgcgctacgc tgaaatgatt
    14341 tattacggct actggttcgc gccggaacgt gaagtgttgc agggagcaat cgacgcggcg
    14401 cagcgcaatg taaacggaac agcgcggtta aagctttaca aaggcaatgt cgttgtcgcc
    14461 ggacgcaaat ccggttcgtc gctctacgat cccagggtgg cgacttttga acaagatacc
    14521 gtttatcaac aggcggacgc cgagggattc atccgcttga acgctctgcg attgcgcctg
    14581 cagaagttga gcaaaatcaa gagatcgtat tgaaggtttc aaaagcaaga caaaaaaaac
    14641 tttggggcgg gcgtttcgct tccgctaccg cggcatccgt ggaagccttc acggcttcga
    14701 tccatatcga ctcgcggctc tatcgatacg atatcatggg cagcatcgcc catacaaaaa
    14761 tgctgtcgaa gcagggcatc attgctgctg gcgacggccg tcggatcgtc cgcggtctaa
    14821 aacagattga agctgagatc gagcagggca actttaactt ttcctcggcg gacgaagaca
    14881 tccatatgaa catcgagcgc cgtttgaccg aaaaaatcgg ctcggccggc ggcatgctgc
    14941 acacagcgcg cagccgcaat gatcaggtcg cgctcgatat gcggctgttc ttgcgtgaag
    15001 aggttcgatc gatcatcgat gacttgagcg cgctgcagcg cgagatcgcc ggtgcggcta
    15061 aacggcacat tgatgtgatc atgccgggat acacccactt gcagcgggcg cagccggtcc
    15121 tgttcgcgca tcatttgctt gcctactacg atatgttcga ccgcgaccgc cagcgcttta
    15181 ctgaatcgct gcaacggatc aatgttttgc ctttgggggc aggagctctt gcgggaacga
    15241 cgtttccgat cgatcgcgcc tacgttgcca gtctcctggg ctttcccgag atttccaaaa
    15301 acagcatcga cgcggtcagc gatcgcgatt ttcttctcga gtttactgcc ggatcttcga
    15361 ttcttttcgt tcacttgagt cgccttgcgg atgaatgggt gttgtggtct agtcaagaat
    15421 tctcgttcat cgagttgccc gacggttact gtaccggaag ttcgatgatg ccgcagaaga
    15481 aaaatcccga tgtcctggaa ttgattcgcg gcaaaacggg gcgggtgttc ggtcatctgc
    15541 aggcgctgct gacgatcatg aagggattgc cgcttgcgta caatcgcgat ctgcaggagg
    15601 acaaaattcc gctgttcgac accgtggata cggtcaaagc cagcttgcag atgatgcgag
    15661 aaattgtccg gggagtaaaa gtgaaggcca atcgaatgtt agctgcggcg caggatggtt
    15721 ttatgaacgc aaccgatcta gccgactacc tggtcgatcg cggcgaaccg tttcgctcgg
    15781 cgcatgaaat cgctggccgt atcgtgcaac attgcatcgc taaggggcga cggattgaac
    15841 aattgccgct tgaagagttg agagattttt cagaaaagat cgaaaaagat gttcatcgct
    15901 acctttcaat cgaaggggtg gttggccgac gtcaggcatt gggtggcacg gcgcgaaaca
    15961 acgtgctgcg tcgcctcaag gaaatccggt ctgcatgaaa tctttcctcg tattcgccag
    16021 tttgatccta ttgctttgta tcggttgcgg taaaaagggt gagccgcgcg ctcccgagtt
    16081 agcgatgccg cagcttatta agaatctcac ggcaaaacct gatcgcactg gaattgtact
    16141 aacctggagc cgccccaccg agtatgtcga cggcaggtca ctcactgatc tgaccgggtt
    16201 tgtcatcttt cgcaaggatc tctcgccgac atgcccggaa tgtccggtgc cgtatcggcc
    16261 cttggctgcc gtgaacgtcg aggatcggga aaaattcgtc aaacaaaagc agtatcgctt
    16321 catcgatgaa aatgcccaag cacagatgat ttatcgctat cgggtgtcgt cgcagttaaa
    16381 ggatggttcg ttaagcaacc catccaatga agttgaggct acgcgaggtc cgtaatgaat
    16441 catttcgaat accggaatgg agaaatgtat gcggaggggg tggcggtaaa acgcatcgcc
    16501 cgggaagtcg gcacgccggc ttatatttac agcttggcga cattgaaacg gcactaccag
    16561 gttttcgatc aggcttttgc caaggttccg cacatcgttt gcttctcggt taaggcaaac
    16621 tcaaatctgg ccgtgctccg aactttcgcc aaggagggca gcgggttcga tattgtttcc
    16681 gcaggcgagc tgtttcgcgc gttgaaggcc ggcggcgatc cgaaaaagat cgtgttctct
    16741 ggcgtgggca agaaaaagga tgaaatcgag tacgccctca gttcggagat tttgatgttc
    16801 aacgtcgagt cggaagacga attggtggct ttgaacgaaa tcgccggcgc catcggcaaa
    16861 aaagctccga tcagtatacg ggtaaacccg gatgttgatc cgcaaactca tccttacatc
    16921 tcgaccggca tgaagaaagc gaagttcggc gtcgaaatca aaaaatcgtt ggacacctac
    16981 aaaaaagccg cagcattgcc caacgtcgat gtcgtcgggg tcgattgtca catcggctcg
    17041 cagctaacgt cgctttcgcc attcgtcgat gccttgacca gggtgcgaga gtatcttgat
    17101 cgcgtgctgg ttggttcgct cgaaaaagag ggcgcacaga ttcgctatct cgatttgggc
    17161 gggggccttg gcatcaacta taacgatgaa actccgccgc agcctgagga atacgccaag
    17221 gcaatcatcg aaggcttggg gggtctcgat gtcacattaa tactcgagcc cggacgcgtg
    17281 attgtcggca atgccgggat tcttgtcacc gaagtgcaat atgtcaaaga caatgaatcc
    17341 aagaaattcg tcatcgtcga cggcggcatg aacgatctca tacgtccggc gctttatggg
    17401 tcctatcagg cgatccgtcc tgtggtggaa acacgagccg agaagatctt cgcggacgtc
    17461 gtcggtccga tttgcgagag cggcgatttt tttgctaagg atcgcgagat cacacggccg
    17521 caacgcggcg atctgttggc cgtcatgagc gccggcgcgt atggctttac tatggcatcg
    17581 aattacaatt ctcatccaaa ggcgcccgag gttctcgttg atggtgacaa gtactacgtg
    17641 gtccgcaagc gcgagtcttt tgaagatttg attaatggcg agacaatacc ggcagcgcta
    17701 gaatgaaatt tcgttcaaag gttcaacgtg acgtgagccg aattagggct aaattcggga
    17761 aaatgttttt caggctgttc aaaaagatct cataggcgag gcgtgcgacg ttcgatgagc
    17821 tgaggcgtac gcttcagtac gttgaagcga atcggacgag tgcaacgaag catatgagtc
    17881 tttttcagca gcctgcaagg tcggtgagat ggagattccg tttaccaaga tgcatggctg
    17941 cggcaatgat tttattttta tcgattgcct gaaaaacgat gtccctgatt tgggcgccat
    18001 ctccggcaga ctctgcgatc gtcgtttcgg catcggcgct gatcagctcc taacggtaca
    18061 tccatctaag atcgccgatt ttaagatgga gatttacaat gctgatggca gccaggtcga
    18121 aatgtgcggc aacggcattc gttgtttcgc caagtacgtt tatgaacacg ggttgacacg
    18181 gaacaaggaa ctggaagtcg agacgttagc cggtatcatt cgtccccgga tcgtcggcga
    18241 tttggtcgaa gtcgatatgg gcgagccaac tctcgaagga cgcaaaattc ccgttgatga
    18301 cgatggccag atcgtaaacc gacccttgat cgtcgacggc aggacttaca ccgtgacgtg
    18361 tgtttcgatg ggcaatcccc actgcgtgac ctacttcgac gatatcgaat cccttgattt
    18421 ggaaaaggtc gggcctggtt tcgagcatca cgctttcttc ccaaaaagag tgaatacgga
    18481 attcattaag gtgctgagcc ccaatgaggt gaacatgcgc gtctgggagc gcggcgcagg
    18541 ggagacctgg gcttgcggca ccggcgcatc ggcagtagcc gtggccggcg tgcttggcgg
    18601 taagaccgag agaaagctga ccgtgcatct taagggcggc gacctgttta tcgagtggcg
    18661 cgacaataat cgtgtttata tgaccggcgc ggccgaagag gtctttcagg gcagggtcaa
    18721 aattagttag gagcagttga tgtttaccgg ttctatggtc gctttgatca caccgtttac
    18781 cggcggcaag gtcgactggc aaagccttga gcggctgatc gattttcata tcgagagcgg
    18841 cacccatggc atcgtgcctt gcggcactac cggcgagtcg gcgaccttga gccatgaaga
    18901 acacgacgag gtcatcaaag ccgtcatcaa agccgttaac cgccagctac ccgtaatcgc
    18961 gggaaccggt tctaattcga cggacgaagc cgtacgctta acacgcgcag ccgaaaagtc
    19021 gggcgcagac ggcgcgctaa tgatttcgcc atattacaat cgaccgacgc aggaaggcat
    19081 ctaccaacac tacaagaagg tcgcttccga agtcggcatc ccgatcatca tatacaatat
    19141 tccgggacgg actggatcaa aaatcgagcc ggagacgctg gcgcgcttgg ccgagatcga
    19201 taatatcgcc ggcgttaaag aagcgactgg ctcggtggat caggcgatcg atgttatccg
    19261 cctttgcggc gacaatctgg ccctttattc cggtgaagac acgctgacgt tttctctgat
    19321 gtccttgggc ggcaaaggtg tgatttcaac agttgccaat gttgcgccaa gagaaatgtc
    19381 acagcttacc gaagcttgcc tcaagggaga gtgggaaaga ggccgcgatt tgcagctgaa
    19441 actgactccg ttgattcgca gccttttcgt cgagactaat ccgattccgg tcaagacggc
    19501 tgctgcgctc atgggcaaat gcagcggcga gctgcggctg ccgctcacgc cgatggccga
    19561 aggcaatctc cagaagctca aacaggcaat gatcgaattc ggcttggtca aacagtccca
    19621 ccatatcgcg aaggcgaacg agagttagcc ggcccctggt tagccgatct aaattcttgg
    19681 catcaatatt tcgtgacggc gaaaaatctc aatcttattg tttgtggtgt cggcggccga
    19741 atgggcggcg ccgtggtacg agcgatccag caaaccgccg gtgtggcgct gattgcaggc
    19801 attgataaat ccgggggcac gaggctggga aaagacgccg gcgaaatttc cgcagccggc
    19861 aatttcggca ttagcgtaac cgacaggatc gagccttacc tcaaaccggg aacagtcatc
    19921 atcgatttca ctcgacctga ggcatcattg acttacctga gggcagcggc gaaaaaggcc
    19981 gtgcccattg tcatcgcgac gacaggattt gatccgcggc aaattgcgga gatcaagcgg
    20041 ttgtcacgcc agacgcccgc tctcttgtcg gccaatacta gcctgggtgt caatgtgctt
    20101 gtgaacctgc tcggcaaggc cgcaaaaatg ctcggcgacg attacgacgt cgagatcgtc
    20161 gaggcgcacc atcggttcaa gaaggacgcg cccagcggca cggccctagc gcttgggcgc
    20221 gctgttgctg gagcgctgag ccgtgatttg gacaaggttg gcattcacgg gcgcaaaggc
    20281 atcattgggg aacgctcaaa aaaagaaatt gctcttctct ccgtccgcgc cggcgacatc
    20341 gtcggcgagc ataccgtgat ttttggcggt atcggcgagc gcctcgaatt catccaccgc
    20401 gcccacaacc gcgatacctt cgctcacggc gccatccgcg ccgcccaatg gctcacacaa
    20461 cagaaacccg gcctttacgg catgcaggat gtgttggggc tgtagtaggt agagtcccaa
    20521 cgtctcattc tctttgctga gcagatcagc atcactcccg aaacagcggc ttcattctct
    20581 tcgacagaac cgccgaacat ggagccgctc caccgattgc caatgcgcct ccgggggatg
    20641 accagag
//
LOCUS       DASUVS010000210         3721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_959243, whole genome shotgun sequence.
ACCESSION   DASUVS010000210 DASUVS010000000
VERSION     DASUVS010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959243"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1643..2200
                     /locus_tag="VF182_11870"
     CDS             1643..2200
                     /locus_tag="VF182_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437822.1"
                     /translation="MNPFYIIAVPLALIIIGMILNNTGLAYDQPPSSQEQDPTKKLAA
                     EKETYRKFFNLQRTRSVTRQKRIGQYSWLVMAAFIGAFIWLYTDTVNKTSLSSRIAAM
                     QTLATEEGKQMVLSVTQSDGTNVKYLIKLPQADKLVETTKAQEAVTKEKVSSWELERL
                     GTALSIGNNSLPNGIALQMSQAEAR"
     gene            2695..3177
                     /locus_tag="VF182_11875"
     CDS             2695..3177
                     /locus_tag="VF182_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX6437823.1"
                     /translation="MTEQWTEWWSRFLELPQDSFRQAINILQQRYAEETQQRDRFNQH
                     ADKMHYPQFRQKLLRISDEKTQYADRIAEAIVALGGKLPSAPELRSTDENSWQQLMIA
                     LDDENRSADHLAEQLRGIQSDIPDVVKLLQHVSEQQKEHRGEVRDMLMRSDPFAFSLA
                     "
     gene            3398..>3721
                     /locus_tag="VF182_11880"
     CDS             3398..>3721
                     /locus_tag="VF182_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX6437824.1"
                     /translation="MSNWATHLFARRAQYSRRAMNLNAPWMLIKDTFVQWLDDNPLQL
                     AAALAYYTLFSMAPLLLIAIAVAGLVFGRDASQLQIIGVIEDFIGVRGAQAIQAIIES
                     AAQRPD"
BASE COUNT          980 a          938 c          954 g          849 t
ORIGIN      
        1 tagaaatctt ttttaaacga ggcgggagtg attgcgggcg tcttcatcgg gggttaccgt
       61 gaataatact cttctgtaca aaaactattt catcggttgt gaatcattcc aacgtgagaa
      121 gcacggtagc tgggttcctc aatacacaat cacccggcaa gtgagtgaat caaacgaatt
      181 tccctcgcat cagtatcaat tcagtcacac attgccgact aagaatgacg ccgatcgtta
      241 cgccgcgcaa aaagcacagg agtggatcga ccagaaccaa aagtcctgat gagtttattt
      301 ggtagaaccc ttcgggatga ggtaatgtca gggaaagtgc gtcgctattc gatttttccg
      361 ccggagcgtt agcgttgatg ggagcggcaa gattgacaag agcctgagat aagattgaag
      421 cactggtcct gtctgccgac aaaatcctct gattcacata ccatcagctt ggtgcgcaac
      481 ggcaccaaaa gctgattgga acctgcaatc cgccttctgg tttgcttctg ttgggagatt
      541 ttcaagattg gttcttttgt tcagtattgg acagcaatcc ctctgcaggg atgcgcaagt
      601 aagcgagtca accggcgata gagcctaacg attggccgtt ttgctagccg caaatcgctc
      661 cggtcgttgc tccgacgggc ggatcatcat ttctctattc tgaccgcgag cactacctgg
      721 cgcattcagg aacacgagat tacgaaggct tggagagtac tttcgaccac ttcccgtccg
      781 tcccagccga gacaggatgc aacgagggcg taggaaccac acacatgtaa ggcgttggag
      841 ttgcggtgtg gccagatacc gaccggcggg gcggacgatt tgcggcgaga aaacattatg
      901 atgattgtgc taggtcactt cctttttgca gcagcagccg cgatcgcaac atcagcgatc
      961 atcgggtatt ttcttactaa attgctgctt tggttacttc aaaaggtgga tcggtcgatc
     1021 agactctaca gaggtaaggt ccctgcgttt tctgcacctc gccgcatctc atcgatgccg
     1081 ctctcaaacg tatcaaaaag cttcgccgca atgaagggga agaagtttgc gctgagtaca
     1141 cagatccgac tcccagagaa aaagtcgtga tatagaaaaa ggcccgcctc aacgccaaac
     1201 aaaatgatgt ataggtcgaa aagagtcctt gccacgattt ggactgcgcc ccagctgtgg
     1261 gggaccaggt cctctaaaaa cgattttaaa gatagataag cggctgaacg cggttctctt
     1321 gaaacatcga aggttgcacg gtggaatgac tgcgcgacca atcgggcatg attcctttgt
     1381 gcaagaattc ttttctatcc tgttcactat tagacaaggc gcaattgctt ccagcctagt
     1441 cgatggttat cttagacatc gacatgcagc tgtgtaatgg attttcgaaa gctgtaatgg
     1501 cgcgcaagag gtaaagaagg ctgggccacg ctctgagcag tggcatgggc ttccgctcca
     1561 gttgactcgc gaacgtattc ccggtccccc ctccctcttc caatatccga aggctattag
     1621 gagcccttca aggagagagt ttatgaatcc tttctatatc atcgccgtcc cgttagcgtt
     1681 gataatcata ggcatgattt tgaacaacac cggacttgct tacgatcaac cccccagttc
     1741 acaagaacaa gatcctacga aaaaactcgc ggcggaaaaa gagacctacc ggaagttctt
     1801 caatctccag agaacgcgat cggtcaccag gcagaaaagg attggccagt atagctggtt
     1861 ggtgatggcc gcctttatcg gcgccttcat ctggctctat accgataccg tcaacaagac
     1921 cagcctttcg agtcgaattg ccgcgatgca aaccttggca acggaagaag gcaagcagat
     1981 ggtcctgtcg gtgacacaga gcgacggcac caacgtcaaa tatctcatta aattacctca
     2041 agccgacaaa ctggtggaaa ccactaaagc acaagaagcc gttaccaaag aaaaggtctc
     2101 atcctgggag ctggaaagac tgggcacggc tttgagcatc ggcaataatt cactgccaaa
     2161 cggcatcgcg ctgcagatgt cccaagccga agccagataa agtccgggat tccaggaatc
     2221 cacatcaggc ttgaggtggc gagttgcacc tcaagcctga gattgttacc tcgacgttta
     2281 gttgtctcca gctagtttct tgaacagatt aaacctggaa taaccgcttg catctgttcc
     2341 aaacgccgag cctagaacag gtcaaacaca taaccgcaat tttgcgaagt ccgccatggg
     2401 taaactgaac ggattaatat tggactgcgt ggatttgaag cggagccgga tcttcggaaa
     2461 tcgaagtctg acggtactga aaagcggcct cgtgttcgag cgtcctaaca caaaagccgc
     2521 ttactgttag attggattac cgctgcagcg gaattggcaa aagcggggag agagcgagcc
     2581 cagagcttac aatcaatggg tgccgaactt catgaggtcc agtcatttgg gcgttcacca
     2641 tacaatcgtg cccggcagac cagctggtgt cagctatggg agaactttaa ggccatgacc
     2701 gagcaatgga cggagtggtg gagtcggttt ttagagttgc ctcaagatag cttccgtcaa
     2761 gccatcaaca tcttgcagca gcgctacgcc gaagaaacgc agcagcgcga ccgatttaac
     2821 caacacgccg acaagatgca ttatccccag tttcgacaaa aactcctccg gatatcggac
     2881 gaaaaaaccc aatatgcgga ccgtattgcc gaggcgatcg ttgcacttgg aggtaagctg
     2941 ccgagtgcac cggagcttcg ttcaacagac gaaaacagtt ggcaacagtt gatgatcgcc
     3001 ctagatgacg aaaatcgcag cgccgatcat ttggcagaac aactgcgagg tatccaatcc
     3061 gacatccccg acgtcgtcaa attgctgcaa catgtttctg agcagcagaa agagcatcgc
     3121 ggcgaagtta gagatatgct gatgcgtagc gatccattcg cgttctcgct tgcctgatgt
     3181 ttcataggac cgtgagttca ttgtcctgtt cggcgccggt acaagtgtaa agatccgaga
     3241 acagccggag ctgctcacgt aaatgcggta attgtgttgc ggttgactcg aggggacaca
     3301 caggagatgt ccatgaaatc gcctaccgtt caaaaaaaac tgcagtcatc ccgctgccaa
     3361 gaaacttctg ccgccgatcc ggcttacgga ttatgacatg agcaactggg ccactcacct
     3421 attcgctcga cgagcgcagt actccagaag agcaatgaat ctaaatgcgc cgtggatgct
     3481 catcaaggat acctttgttc aatggctcga cgataacccg ctccaattag cggccgcgct
     3541 ggcttattac accttattct ccatggctcc tctcctgttg atcgcgatcg cggtcgctgg
     3601 cttagtgttc ggacgggacg cttcccaact tcagattatc ggtgtgatcg aggatttcat
     3661 cggagttcgt ggcgcgcagg caatccaggc tataattgaa agcgcagcgc aaaggcccga
     3721 c
//
LOCUS       DASUVS010000211        16447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_86468, whole genome shotgun sequence.
ACCESSION   DASUVS010000211 DASUVS010000000
VERSION     DASUVS010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 16447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16447
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86468"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VF182_11885"
     CDS             <1..366
                     /locus_tag="VF182_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6437825.1"
                     /translation="LAPAVPEIAIRQFLMKNIARASSGGFQWRIDLASIVKNYDNLTK
                     PIVAAAAYDKPALFIRGGRSDYIADVDLASIKAIFTQAELVTIARGGHWLHADATYEF
                     LAILNDFLNSHTVDHRTLR"
     gene            532..912
                     /locus_tag="VF182_11890"
     CDS             532..912
                     /locus_tag="VF182_11890"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6437826.1"
                     /translation="MAIRKSIHIQGMEHGAPIPNGVVLGNLVFSSAISGKDAKTGVLS
                     EDPAEQARAMFRNLELFMEKAGGASDNIAHMKVYLKEEKYRDAVNQAWLEMFPDEHDR
                     PARHALKVELRGNVLFQIEVVAVL"
     gene            961..1347
                     /locus_tag="VF182_11895"
     CDS             961..1347
                     /locus_tag="VF182_11895"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6437827.1"
                     /translation="MSVDIIDLVDLAHQEKRRKNIFNTPRFHAWMHYYQPGHKDEMHC
                     HNADQTFYVIEGECTMRFPDGGQVVLKPGQAALITGGSFYQLENAGSEPMIMMGNRSG
                     PAEKIQIISYETRKDIRQQVQTEFNT"
     gene            1625..2899
                     /locus_tag="VF182_11900"
     CDS             1625..2899
                     /locus_tag="VF182_11900"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6437828.1"
                     /translation="MVEPLSLDDNKTLVESHFGFHGMPFGVAPDPRFFFRNACYAEGL
                     DILSEGIKAKKGLLLVTGEVGTGKTILLRKLMRHLEASTRFVFVSTSHLNSVGLIQLI
                     VEDLGLPTEGRTAFELAQALKEYVLQQNRAAKNVAVLIDEAQKLTDGALEGLCDLSNL
                     ETEQEKLLQIILAGQPELTTKLCQPALQRIKQRIAIHYQTRRLSLASDVEHYVRHRLG
                     ASGYEGPEIFNRKALEAIWLYSAGTPRLINTLCDNALAMASRAGRKRVSGYLIVKVAA
                     KLMLERPTDGRTNGASHNGNGIPILPTGDLGAPTSQASPQAAGISRSFINQRLSRVAA
                     PLSDPKERTVASQFFDFMARAAAEGIGPMAKLIIDDQIADLGESRDSFPQKKLGDLIE
                     RVSREIFNDTMRALFLDKMAQAVSALRSIRAF"
     gene            2940..3686
                     /locus_tag="VF182_11905"
     CDS             2940..3686
                     /locus_tag="VF182_11905"
                     /inference="COORDINATES: protein motif:HMM:NF028512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX6437829.1"
                     /translation="MGAESGVSLKWKIGGVYTAVMLILGLLLIAALYQLTRTAMREQL
                     DKRAVAIATNFSDAAASHIVSKNQLALYALAAKYTLFEGVAYVYINDPRGEIIAHTLG
                     SFPQQLRQGAVAGAQREPQRRTLSLQEKTVYETSVPVLDGQAGTAHVGFWSDAVDKEV
                     RSFIWPMIGVIALVPLVGAALSFIIANWIVRPIIGLTEVADKVTKGDLEMSIGNSVVS
                     RDEIGELARSLERMRSSLRAAMLRLNREAT"
     gene            3716..5050
                     /locus_tag="VF182_11910"
     CDS             3716..5050
                     /locus_tag="VF182_11910"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437830.1"
                     /translation="MHYSKKYSPLRLLIFVTIALFLPAGAYSADTPERALEKITIAYS
                     SVSGNMAPLWITHDRGFFRKNGLDVQIVFIESGSTAVQSLSNKDVAFAQMAGAGVLQS
                     RLRGSDVLMIAGFLNTLDYQLMVEKNITRPEQLKGKSMAVSRFGSSSDFATRYAIEKF
                     GLVPEKDVPILEIGSQPARFAALESGKIQAAMIAVPLTARAKALGFHALADLQMLGLE
                     YQHTGLVTTQALIKSRPDIVRNVMKAYVEGIHYYKTNRPESIAILAKYLKTNDSELLN
                     EVYTDLGLTLTPQKPYPTLRGIDIMLRELAGRDAKAKTARPEEFVDLTFIKELDSSGY
                     IDRLYKTQPVVAKRQEAPAAAPATPSAKAAAQPKAAPPPAKVATAKPAAAVDAPSEGS
                     QEYTVVSGDTLSHLAQRFYGDHYRWNKIFEANKATMKNPNFLFIGQKIFIPS"
     gene            5168..5560
                     /locus_tag="VF182_11915"
     CDS             5168..5560
                     /locus_tag="VF182_11915"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6437831.1"
                     /translation="MAQIKHIAIRTRDVDRTAAFYKDAFGLTQVGMGKNGVYLTDGHL
                     NIAILKYQQGKDGQPLRLGVDHVGFQVADLDSALAQIELLRGTRLTEPTEVTPVNAAE
                     PQSYYEIKCVGPDEQVIDISSIGWVGAK"
     gene            complement(5660..6469)
                     /locus_tag="VF182_11920"
     CDS             complement(5660..6469)
                     /locus_tag="VF182_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6437832.1"
                     /translation="MLKDFLPFIWLIPLGLLAGAYGALIGAGGGFILVPALLILFPNE
                     APEIVTSISLMVVFFNALSGTIAYARSKRIDYKIGIIFSLATMPGAVLGALATTAVSR
                     ERFNLLFGFLLLAIAVVLVISPGKDSATRIAQINYRSSSLRSLDTWTLIIGAPVSTAF
                     GFLSSFLGIGGGILYVPALVYLLRVPVHVATATSLFVLTITAFTGSITHLITGLFHAG
                     MARAVALSLGAVLGAQAGAMLSQKIHAEWIIRSLALALGLVSIRLLSQSLW"
     gene            complement(7018..7581)
                     /locus_tag="VF182_11925"
     CDS             complement(7018..7581)
                     /locus_tag="VF182_11925"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6437833.1"
                     /translation="MKVRMVGSVSALLLVLTISLAWSQDPIPYVPSTMPVVERMLELA
                     EVRKDDFVYDLGSGDGRILIQAAKKYGARGVGIDLDPQRVAEATQKAKEEGVSHLVEF
                     RAEDGTQTDISKATVVTLYMFKWFNNAIKPKLQKLKPGSRIVAHDYDIDDWEPTAVVQ
                     LEPTKDPGIERHHTLYLWKVGIESAQR"
     gene            7782..8099
                     /locus_tag="VF182_11930"
     CDS             7782..8099
                     /locus_tag="VF182_11930"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HEX6437834.1"
                     /translation="MSIPTVWSYLSVNLLLQAFDGVLTYRVLSLGIPEANPLVSASIA
                     QWGELWGLLLWKMLACVLLLVIFRFRHQRQSLTIQAYRITGGVYGWFGVLSLCELLVN
                     LDV"
     gene            complement(8326..11544)
                     /locus_tag="VF182_11935"
     CDS             complement(8326..11544)
                     /locus_tag="VF182_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX6437835.1"
                     /translation="MQKLAEVSIRRPVFASMIVLALVVVGAASYFRLGVDRFPSVDLP
                     NVSVRTTLPGASPEEVETVISQPIEEVVNTVEGIDELRSVSGRGTSFVIATFNLDRDI
                     DVAAQDVRDRVATVLNRLPREVDPPVISKFNNEDSPILAIAVSGDRSLRELTEIADKI
                     VKVQIERSIGVGEVRIVGGLERAINIWIDADRLAAYQIPITAVRDAIQRQNADIPGGN
                     VTAGAREQVLRTMGRIVVPEAFNDLVVSTVNGVPIRIRDIGSAEDGTKEQRSFARLNG
                     VPTVQLQVRRQSGANTIAVIESVKENLQRVRRQLPGDVKTEIIRDQSRYIYAALHEIT
                     FHLILGSLFACLVVLAFMRSWRSTIIAAVAIPSSVISAFGMMWALDFTLNSVTMLALV
                     LMVGIVIDDAIVVLENIFRFVEEKKMNPFEAARAATAEIGLAVMATTFSLVIIFVPVS
                     FMSSISGRFLYQFGLTASVAVLVSLLVSFTLTPMMSARMLSAADAHAAGEDHHARSRR
                     GFYGWIDRHYERMLGWSLAHRFAVMLFAGLVVLSSVPLYMLVRQEYIPTDVDEAEFEV
                     NITAPQGTSLAAMDEIMRAVETELRATPLVRLMLCDAGGGFISGVGSGGCYVRIALHA
                     ERIFSFGRLWRETLDGRPWAAFSNVSQRDIMQEVRVRLSRFSDLRTSVRNLPSFNIGG
                     GNWEIDFVLRGPDLKALADYAERLRIRSHDLGIIDADTTLKLDNPELRVVIDRKRAAD
                     LNVDTEHIASALRLMVGGDEEVSRFIDPAVNEEYDVQLRLSPHDREDIGVISRLYVPR
                     AGAGLVRLDNLVQVEPAQSASRIDRLDRQRQVSLRAQIAPGYALADRIEALNGAVREM
                     NLPAEYTTSISGRGRELQRTFNQFLWAFLLSIIFMYMILASQYESTIHPLTILLSLPL
                     SVPFAFLSLWLTGNSLNLYSALGILVLFGVVKKNAILQIDHMNRLRQEGTERMPAIMR
                     ANRDRLRPILMTTLALVAGMLPLALGSGPGAEERRAVAVVVIGGQSLCLLLTLLVTPV
                     AYSTFEDAAALLFSKRAAAPGRLFNLRRRFIGSWKSQRPPIR"
     gene            complement(11544..12764)
                     /locus_tag="VF182_11940"
     CDS             complement(11544..12764)
                     /locus_tag="VF182_11940"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX6437836.1"
                     /translation="MTWLRSTGQRRRGLLSAALLLPLALAGCGGDQSATAVIESSPAP
                     RQVKIATAVEAKVARTVSATGTLAAEDQVVLGTKVVGRLGEISVDLGSRVRKGQAIAR
                     IDPSDYRLRVDQAEAALQQARVRLGLPAEGTSDKIEPEQTATVRQAAATLKEARLTHD
                     RMAKLWEGNYIARAELDAAVAQLAIAESQYQNALEEVRIRQGLLFQRRSELEIARQQL
                     ADTVIVSPMDGAVSERQGSVGQYLTAGAPVVTLVRMDPLRLRLAVPERQAGSVHVGQT
                     VELMVEGDSQRYSGRVVRLSPAISENNRTLLIEAEVANSNGRLRPGSFAKADIIVEAG
                     EQIVTVPRDAIITFAGIDKVLSVEEGQAVEKRVRTGRRIGDQIEIVEGVVAGYQVIVD
                     PGNLVAGQPVKVMN"
     gene            complement(12761..13507)
                     /locus_tag="VF182_11945"
     CDS             complement(12761..13507)
                     /locus_tag="VF182_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HEX6437837.1"
                     /translation="MFNHQDKHSTELSSATERRLLEAAGEIFAEVGYRAATVRQICEK
                     AGANIAAVNYHFGDKEGLYMAVLRSVPDAHAEKFPADRGLSPGATAEQRLAAYIESLL
                     NRVFDEGRPGWHTKIMAREMIEPTRALDTLVADVALPVHQELASIVRELLGSRASEDA
                     VRLCTLSILSQCVYYHHARSVLSRLYPEQEYGSPDIVLLADHITRFSQGALQRFAQGN
                     GAGSSAKSRRALQPARTHPVRTKRVRRIDR"
     gene            13642..14052
                     /locus_tag="VF182_11950"
     CDS             13642..14052
                     /locus_tag="VF182_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6437838.1"
                     /translation="MAKLRHLALLTHQPEKLADFYKKVFELEEVFRTKNGSVHLSDGE
                     VNLAILNANDPKDADDLKHMGMYHFGFHVDDQETVANRIQEIYPQGAPKKRPNGTSYA
                     ETRGTDPDGNIFDISTWGWSGKPLPKDKGGPEQQ"
     gene            14073..15152
                     /locus_tag="VF182_11955"
     CDS             14073..15152
                     /locus_tag="VF182_11955"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6437839.1"
                     /translation="MTKFLLGLLLILATFSIHSNSGAATVADVLKKIQPLQPPQRKAA
                     LEEGAKKEGQVVIYTSVSLSDYPKILSAFEQAHPFIKTHAFRSTPSGVFRRVDTEAKT
                     GRFAVDLVGSAPIEVWQLKERQLSTPYASPERGNLFKGAFDADSYWTAFDITPIVLAY
                     NTKLVAAPEVPKNYQDLLNPKWRGKMSLGTEDYDWFSVMLDHMGKEKGLEYMNSLAKQ
                     NLHMPGSSSVMRVQLLLAGESSIAIAARGRRVAELKSKGAPIDFRIFDPYAGEPDLLA
                     LLQRAPNPHAAILFYDWLLSQDGQTKMSDQTGRISVRKGIKHQPWLQELLQKDFVFVT
                     PTSRAVNLKEVTELYHQVFGLHQAR"
     gene            complement(15252..15746)
                     /locus_tag="VF182_11960"
     CDS             complement(15252..15746)
                     /locus_tag="VF182_11960"
                     /inference="COORDINATES: protein motif:HMM:NF017517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEX6437840.1"
                     /translation="MFRRVDLPARIPGTLLLHSMPGRFEAIERVWRQVQIDAVRAIVC
                     LTEKYEIRLKSSNYADALEAGAVPCSVLPFEIREGGVPADRQAFWQLARDLAERLQSG
                     EALLIHCAGGVGRTAMLAVSVLLALGQPFDEAESAVSRAGSIVETMPQIEMLSWCAAQ
                     VEIK"
     gene            15947..>16447
                     /locus_tag="VF182_11965"
     CDS             15947..>16447
                     /locus_tag="VF182_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437841.1"
                     /translation="MKKIFCFLLIALFSFLSENSFAHFTGKGHVHTLSETKQDYLNSD
                     CRPTASCDLKRFTLTTSVYEVWFSDDPNYPTYGNSVIMEYQTDSVAALERYAIVQFKK
                     GCVFYTSKTPAGNIDTKVNDTVPSFGEDVPFCFPRWVIDSQDTDPAYNSDPETGRFYL
                     LRWNKPG"
BASE COUNT         3923 a         4646 c         4425 g         3453 t
ORIGIN      
        1 ctcgcaccgg ccgttcccga aatcgcgatc aggcagtttc taatgaaaaa tattgccaga
       61 gcgtcgtccg gcggttttca gtggcgaatc gatcttgctt cgatcgttaa aaactatgac
      121 aacctgacta agccgatcgt cgccgcagcg gcctatgaca aaccggctct cttcatccgt
      181 ggcgggcgat ctgattacat cgcagatgtc gacctggcct cgatcaaggc gattttcaca
      241 caggctgaac ttgtgacaat cgctcgtggc gggcattggc ttcacgccga cgctacgtat
      301 gaattccttg cgattctgaa cgattttcta aacagccata ccgtcgacca tagaaccttg
      361 cgctagagtc attgaaccgc cggcgtctct ctcttaacca tcccgtcaca ctgcgcgagc
      421 ttcatctcac gacatttcgc caaaacaatg ccatcccgcc gggaccatag accatggtgg
      481 gagggcgtgc tatgacacag ctgactcttt ggagcgaagg aggcttctct catggcaata
      541 cgcaaaagca ttcacatcca aggcatggag cacggcgcgc ctattcctaa cggcgtggta
      601 ctcggcaatc tggttttctc ttcggcaatt tccggcaaag acgccaaaac cggcgtgctt
      661 tcggaggatc cggcggaaca agcccgcgcc atgtttcgca atctcgaact attcatggag
      721 aaggccggcg gcgcgagcga caatatcgcg catatgaagg tttatctaaa agaggaaaag
      781 tatcgtgatg ccgtcaatca ggcgtggctc gagatgtttc ccgatgaaca cgatcgcccg
      841 gcccgccacg cgctcaaagt cgaattgcgc ggcaacgtgt tgtttcagat tgaagtcgta
      901 gctgtgctat aagtttcttt gacggtcgga tctggccaat attataatca cggaggtttt
      961 atgagtgtcg acattatcga tctagtggat ctggctcacc aggaaaaacg gcgcaagaac
     1021 attttcaaca cgccgcggtt ccacgcttgg atgcactatt atcagccggg gcataaagac
     1081 gagatgcatt gtcacaatgc cgatcaaacc ttctatgtga ttgagggtga atgcaccatg
     1141 cgttttcccg acggcggtca ggtcgtgctg aaaccgggcc aggcggcgct gatcacggga
     1201 ggatcattct atcaactgga gaacgccggc tccgaaccga tgatcatgat gggcaaccgc
     1261 tcgggaccgg ccgaaaaaat tcaaatcatt agctacgaga ctcgcaagga tattcgccag
     1321 caagtgcaaa ccgaattcaa tacctgagat ttctgtcagg cgggtagcgg tgccatgcat
     1381 tccaggcttt acgagaaccg caatcagaag cgccggcttt ctctgaagag ctcgcctggc
     1441 ccaggacggc tcagaagctt cttcttgaca agtaaaacaa aaatctacta tgaattgctg
     1501 ctcgcgccac cgaccgcaaa cttcagcacg tacgacagtc aaggttaatc acgggccacg
     1561 atttcaggca ccggccggtt tgatttcatc cgatcgcagc gaggttggga agacggggga
     1621 gtgaatggtc gaacctctta gcttggacga caacaaaacc ttggtagaaa gtcacttcgg
     1681 atttcacggc atgccttttg gcgttgcccc ggatccccgc ttcttctttc gaaacgcttg
     1741 ctatgccgaa gggttagata ttctttccga aggcattaaa gcaaaaaaag ggttgttgct
     1801 cgtcacgggt gaagttggaa ccggaaagac tattctgttg cgcaaactga tgcgccatct
     1861 cgaagcaagc acacggttcg tcttcgtttc gacaagtcac cttaacagcg ttggactgat
     1921 ccaattgatc gttgaagatt taggcctccc cacggaaggc aggaccgcgt tcgaactagc
     1981 gcaggcgctc aaggagtacg tgctgcagca gaatagagca gcaaaaaacg ttgcggtatt
     2041 aatcgacgaa gcccaaaaac tcactgacgg agcattggaa ggcctgtgcg atctctcgaa
     2101 cttagagaca gaacaggaga aactgcttca aatcatcctg gcgggacaac ctgaactgac
     2161 caccaaactg tgccagccgg ctctgcaaag aattaaacaa agaattgcaa ttcattatca
     2221 aactcgccgc ctgtcgttag ccagtgatgt ggagcattac gttcgccatc gtctcggcgc
     2281 gtccggttat gaaggtccgg aaattttcaa tcgaaaagcg cttgaggcga tctggttata
     2341 ctccgcagga acgccgcgat tgatcaacac cctgtgcgac aacgcgctgg cgatggcctc
     2401 gagagcaggc cgcaaaagag tctccggtta cttgattgtc aaggtggcgg caaagctcat
     2461 gttggagcgg ccgaccgatg gtcgaaccaa cggcgcgtcc cataatggta atggcatccc
     2521 tatcctccca accggggatc tcggcgcgcc tacttctcaa gcgagcccgc aagccgccgg
     2581 cataagtcgc agcttcatca accaacgatt aagccgagtg gcggcgccgc tgagcgatcc
     2641 caaggaacgc acggtagcgt cgcagttttt tgacttcatg gctagagcag ccgccgaagg
     2701 cataggaccc atggcaaagc tgatcatcga tgatcagatt gccgatcttg gcgagtcgcg
     2761 cgacagcttt ccacaaaaga agctcggtga ccttattgag cgagtcagcc gtgagatttt
     2821 caacgatacg atgagggctc tctttctaga taaaatggcg caggcggtca gcgcgcttcg
     2881 atcaatccga gccttttagt taccggtact gggtttcatt cgaaggagaa gataattata
     2941 tgggtgcaga atcaggtgtc agtttgaaat ggaagatcgg cggagtctat accgccgtca
     3001 tgttgatctt ggggctcctt ctaatagccg cactctatca gctaacgaga acggccatgc
     3061 gagagcaact cgacaagcgc gccgtcgcca tcgccaccaa ctttagcgat gccgctgcca
     3121 gtcatatcgt cagtaagaat cagctggcgc tctatgcgct ggccgcgaaa tacaccctct
     3181 ttgaaggagt cgcttacgtt tacatcaatg acccgcgcgg cgagatcatt gctcatacgc
     3241 tcggctcttt tccgcagcaa ctgcgccaag gagccgttgc gggagcccag cgtgagccac
     3301 agcgccggac gttatcatta caggagaaaa cggtttatga gacaagcgtt ccagttctcg
     3361 acggacaagc gggaacggct catgtggggt tctggagcga cgcggtggac aaggaagtac
     3421 gaagctttat ctggcctatg atcggcgtca tcgcgctggt tccgcttgtc ggtgcggcgc
     3481 tctcttttat tatcgccaac tggattgttc gacccattat cggtttaact gaggtggccg
     3541 acaaagtcac caagggtgat ctggagatgt cgatcggcaa cagcgttgtc tctcgcgatg
     3601 aaatcggtga gttggcgcgt tcgcttgaac ggatgcgctc gagcctgaga gccgcgatgc
     3661 tccgtctgaa tagagaagcc acgtgacttt ctcaaactcc catgggagga tgaatatgca
     3721 ttactcgaag aaatatagcc cgctacgatt gctgattttc gttacgatcg cgctgttctt
     3781 acctgcgggc gcttacagcg ctgacacgcc ggagcgagcg cttgagaaga tcaccatcgc
     3841 gtacagctct gtttcaggca acatggcgcc cttatggata acccacgatc gcggcttctt
     3901 tcgcaaaaac ggtctcgacg tgcaaatcgt cttcatcgaa agtggcagca ccgcagtgca
     3961 gagcttgagc aataaagacg tggcattcgc ccagatggcc ggagctggcg tgctgcagag
     4021 ccgcctcaga ggatccgacg tgctcatgat agccggtttt ctgaacactc tggattatca
     4081 actgatggtt gagaaaaaca tcaccagacc tgagcaactc aaaggaaaat ctatggcggt
     4141 aagcaggttt ggttcttcct cggatttcgc cactcgctat gctatcgaaa aattcggcct
     4201 ggtgccggaa aaagatgttc cgatcctgga aattggatct cagccggcgc gatttgcagc
     4261 gcttgaatcc ggcaaaatcc aggcggcgat gatcgccgtg cccctaaccg ccagagccaa
     4321 agccctcggc ttccacgctc tcgccgatct ccaaatgctc ggtctagaat atcaacatac
     4381 cggcttggtg acgactcagg cgctgatcaa atcccggcct gacattgttc gtaatgtaat
     4441 gaaagcttac gttgaaggaa ttcactacta caaaaccaat cgccctgaat ctatcgctat
     4501 cttagcaaaa tacctaaaga ccaacgactc ggaattgttg aatgaagtct ataccgactt
     4561 aggcctgacg ctcacgccgc agaaacctta tccaacccta cggggcatcg acattatgct
     4621 ccgcgaactg gccgggagag atgcaaaagc taaaacagcg cggccggagg aatttgttga
     4681 tctgacgttc attaaagagc tagatagctc cggttatatc gatcgccttt ataaaaccca
     4741 gccggttgtg gccaagcgtc aggaggcccc tgccgcggca ccggccactc ccagcgccaa
     4801 agcggcggcg cagcccaaag ccgcgccgcc tccagcgaag gtcgccacgg cgaagccggc
     4861 tgctgctgta gatgctccca gtgagggttc gcaagaatac accgtggttt ccggagacac
     4921 tctgagccac ctggcacagc gtttttacgg agatcattac aggtggaaca aaatctttga
     4981 ggcgaataag gcgacgatga aaaatcccaa ttttttattc atcgggcaaa agatattcat
     5041 tccctcgtga tttgaagtga gttttcgaat actgcatgac gcacggcgta gtaagtttac
     5101 cttaatatcg ctgccgatct tcccaaacga caacgagcgc atggtttgaa ggcggaggag
     5161 tgaagcgatg gctcagatta agcatatcgc tattcggact cgcgatgtcg atcgaacggc
     5221 cgctttttac aaggacgcat tcggtcttac ccaggtcggc atggggaaga acggcgtcta
     5281 tttaaccgac ggccatctca atatcgcaat tctgaaatac cagcagggca aagacggcca
     5341 gcctttaagg ctgggcgttg atcatgtcgg tttccaagtc gcagatcttg atagcgcttt
     5401 agcccagatc gaattgttgc gcggaactcg attgacggaa ccaaccgaag tcacccctgt
     5461 taatgccgca gagccgcaat cctactatga aatcaaatgc gtcggtccgg atgaacaggt
     5521 aattgatatc tccagtatcg gctgggtagg ggccaaatag cgatatagtt cgcgagtcac
     5581 cggggacaac acgctttgcc ccgagcgaac agccaaatac gcaccgcaat cacgtaaccg
     5641 gcctaaacga tcctggcgat caccacaatg attggctgag cagtcgaata ctcactaggc
     5701 caagggctag tgcgaggcta cgaatgatcc actcggcgtg aattttctgc gacagcatgg
     5761 cgcctgcctg cgctccaagc acggcaccca gcgaaagagc aaccgcacgc gccattccgg
     5821 catgaaaaag ccccgtgatc aaatgagtga tgctgccggt gaacgccgta atcgtgagaa
     5881 cgaacaatga agtggccgtg gcgacgtgga ccggaaccct caagagataa accagggcag
     5941 gaacatagag aataccgccg ccgatgccca agaaactcga caagaatcca aaggccgtgc
     6001 tcaccggtgc accgatgatt agagtccaag tgtctaaact gcgcaggctt gatgatcgat
     6061 aattgatttg cgcgattcgg gtagcgctgt ctttgccggg actgatcacc agaacgacag
     6121 cgattgcgag caaaagaaag ccaaacaata gattgaatcg ttcccggctc accgccgttg
     6181 tggccagtgc tccaagcacg gctcccggca tggtcgcgag cgagaagata atgccaatct
     6241 tgtaatcgat ccgtttcgaa cgcgcgtaag cgatggttcc ggagagcgcg ttaaagaaaa
     6301 ccaccatcag cgaaatgctc gtgacaattt ccggagcctc gtttgggaac aggataagaa
     6361 gcgccggcac taagataaaa ccgcccccgg cgccaatcaa cgcgccataa gcgccggcaa
     6421 gaaggcccag cgggatgagc cagatgaagg gtagaaaatc tttcagcatt cagttccgcc
     6481 tgtgcatggt tccgattgac ctgcatgatt gaaagctgct cactccacag cctttgctcc
     6541 gtttactgat cggccaaaag cgccggcatt taaccatcga ccacttgggg cgatcaggat
     6601 ttgcatcttg cgcgagtaac gaacggctgt actccccgcc actggtgatg gaaacaacgt
     6661 cggagtcgcc tcgactccac gcaccaggcc gagctgccgg cgccgaagcg gttagatcat
     6721 aaatcatcca gccgagcgat atctaatcca tttagaaagc gagcgaacta aaggaatggc
     6781 cgagccatag cagccgaaat cactataagc ggagcgcctc cacggcgcga attttcggga
     6841 atcaaacgtg ctgtcgacgg atctaaagag cgctttgtcg aactcaaagc agtggttacg
     6901 gacttctcgc tgatccggcg atccgcgatt ggggcgatgg aatattcgat tttggatctt
     6961 actctaagcc cggcgccgag cagatgttga acttcgaaga tttaacccaa gattgggtca
     7021 ccgctgcgcc gactcgatcc cgactttcca caggtagagg gtgtgatgtc tttcaattcc
     7081 agggtccttg gtcggttcca actgtacgac agctgtcggc tcccaatcat cgatgtcgta
     7141 atcatgggcg acgattcgcg atcccggttt taacttctgc agcttaggtt tgattgcgtt
     7201 gttaaaccat ttgaacatgt acagtgtcac cacggtggct ttcgaaatat cggtctgagt
     7261 gccatcctcc gcgcgaaact cgacgaggtg actcacccct tcctctttgg ctttttgcgt
     7321 cgcctcggcc acccgttgcg ggtccagatc gatgcccacc cctctggcgc cgtacttttt
     7381 tgctgcctga atcagtatcc gtccatcgcc gctgccgaga tcgtaaacaa aatcgtcctt
     7441 ccgaacctcg gcgagctcga gcatccgctc gacgaccggc atcgtgctcg gcacataggg
     7501 aatcggatcc tgcgaccaag ccagcgagat ggtcaagacc aacaacagcg ccgaaactga
     7561 gccgaccata cgaaccttca tgtgggaacc tcgcatttaa aatgaatttc gaacatgcct
     7621 atcgggtcaa cgtatttggg atggctttta attgcaacct atttgcctgt tcagcatcgc
     7681 cggcgagcgg taaagattct gctgcgcagt ttgtcgcttg acaacagcgg tcacgcttgt
     7741 ttggtttaac attaggacaa cttctttgat gaggcatcgt tatgagcatt ccgacggttt
     7801 ggagctatct cagcgtcaac cttttactgc aagcgtttga cggggtttta acctatcggg
     7861 ttttatcgct tggcataccc gaagcgaacc ctcttgtgag cgcctccatc gctcaatggg
     7921 gcgagctgtg gggcctactt ctgtggaaga tgcttgcgtg cgtcttgctg ttagtcattt
     7981 ttagattccg ccaccagcgc cagagcctta cgattcaggc ttacagaatc accggtgggg
     8041 tctatggctg gttcggcgtt ttgtctttgt gtgagttgct ggtaaatctc gacgtttgat
     8101 ccgtttcgaa ctcggcgaaa cttcggggtg aggctaagca ggtcctgacc gggcttcgcc
     8161 tcgcccctga ctccttcttc gattcgaaat agcgatccga tgtcgctaat cggtgccggc
     8221 acgttttgcc cgcagtaggg ccgccacggc tgatcccgac ccaaccagcg ctgacccagt
     8281 aaaggacagg gtgtaagaaa gctcgggtat aaagacgatt aaaagtcagc ggatcggcgg
     8341 ccgctgcgat ttccagctgc cgataaatcg gcggcgcagg ttaaagagcc ggccgggggc
     8401 cgctgcgcgt ttcgaaaaca ataatgccgc ggcgtcttca aacgtcgaat acgccaccgg
     8461 cgtcaccagc aaggttaata gcaggcagag cgattgtccg ccgataacca cgaccgccac
     8521 agcccgccgc tcctcagcgc ccggccccga tcccagagcc agcggcaaca tgccggcgac
     8581 caaggccagt gtggtcatca gaatcggccg caaccggtcg cgattggcgc gcatgatggc
     8641 gggcattcgc tcggttccct cctggcgcag ccgattcata tggtcgattt gcaggatcgc
     8701 gttctttttg acaacgccaa agagcaccag tatgccaagc gcggaataga gattgaggct
     8761 gttgccggtc agccacaatg acaggaacgc aaacggcacc gacagcggca gcgataaaag
     8821 aatcgtcaac gggtgaatcg tgctttcata ctgcgatgcc aggatcatgt acatgaaaat
     8881 gattgaaagc aaaaacgccc ataaaaattg attgaaggtg cgctgcaact cacggcccct
     8941 ccccgatatg ctggtggtat attcggcggg tagattcatt tcacggaccg ccccattcag
     9001 cgcctcgatg cgatcggcca gcgcataacc gggagcgatc tgggcgcgca ggctcacctg
     9061 gcgttgccgg tccaggcgat cgatccgcga cgcgctctga gccggctcca cttgcacgag
     9121 attgtcgagg cgcaccagcc cggcgccggc gcgcggaaca tacagccggg aaatcacccc
     9181 gatatcttca cgatcgtgcg gcgacagacg caattgcaca tcgtattcct cattaaccgc
     9241 cggatcgatg aatcgcgata cttcttcgtc gccgcccacc atcaaacgca gcgccgaggc
     9301 gatatgctcg gtgtcgacgt taagatccgc ggcacgcttg cgatcgatta ccacccgtaa
     9361 ttcggggttg tcgagcttga gcgtggtatc ggcgtcgatg atgcctaaat cgtgggagcg
     9421 aatgcgcaaa cgctcggcat aatcggccag cgccttgaga tccggcccgc gcaagacgaa
     9481 gtcgatctcc caattaccgc cgccgatatt gaacgacggc aggttgcgca cggaggtgcg
     9541 aagatcggaa aatctgctta aacggacgcg aacctcctgc atgatgtcgc gctgcgaaac
     9601 gttactgaag gccgcccaag gcctgccatc aagcgtctcg cgccagagcc ggccaaacga
     9661 gaatattcgc tcggcgtgca gcgcaatgcg cacatagcac ccgcctgagc caacgccgct
     9721 gatgaaaccg ccgccggcat cacaaagcat cagccggact aacggagttg cccgcagctc
     9781 ggtttccacg gcgcgcatga tttcatccat tgccgcgaga ctggtccctt gaggcgcagt
     9841 tatgttcacc tcgaactcgg cctcgtcgac atcggtgggg atgtattcct gtcgcaccaa
     9901 catataaagc ggaactgaag agagcacgac caaaccggca aataacatca ccgcaaaacg
     9961 gtgcgccaga gaccacccga gcatgcgttc gtaatggcgg tcgatccagc cataaaaacc
    10021 gcgccgcgag cgcgcgtgat gatcttctcc tgcggcgtgc gcgtctgcgg cgctgagcat
    10081 gcgcgcgctc atcatcggcg taagcgtaaa agataccaac aagctgacca agaccgccac
    10141 cgaggcggtc agaccgaatt gatagaggaa tcgtcccgag atgctcgaca tgaacgagac
    10201 cgggacgaaa ataatcacca ggctgaaggt cgtcgccatg accgccaagc cgatttccgc
    10261 ggtggcggcg cgcgccgcct cgaaaggatt catcttcttt tcctcgacga aacggaagat
    10321 gttttccagc acgacgatgg catcatcaat gacaatgccg accatcagca ccaacgccag
    10381 catggtgacg ctgttcagcg taaaatcgag cgcccacatc atgccgaagg cggaaatcac
    10441 cgaactggga atcgccaccg ccgcaatgat cgttgagcgc cagctgcgca taaaagccag
    10501 caccaccaaa caggcgaata gactgcccaa aatcaggtgg aaagtaatct cgtgcagcgc
    10561 ggcgtaaatg taacgggatt gatcccggat aatctcagtt ttgacatcgc ccgggagctg
    10621 gcgcctgacg cgctgcaagt tttccttgac cgactcgatc acggcgatgg tgttcgcgcc
    10681 cgactgtcgc cgcacctgga gttgcacggt cggcacccca ttgagccgcg cgaaggatcg
    10741 ctgttccttg gtgccgtctt cggccgaacc gatgtcgcgg atgcggatcg ggacgccgtt
    10801 tacggtagaa accaccagat cattgaaagc ttccgggacc acaatccgcc ccatcgtgcg
    10861 aagcacctgt tcccgggcgc ccgcggtcac gttgcccccg ggtatgtcgg cgttctgccg
    10921 ctgaatcgca tctcgcacgg cagtgatggg aatttgatag gccgctaaac ggtccgcgtc
    10981 tatccagatg ttgatggcac gttccaagcc gccgacgata cgaacctcgc cgacccctat
    11041 cgagcgctca atctgcacct tgacgatttt atcggcgatc tcggtcaatt cgcgcagcga
    11101 ccggtcgcct gaaacggcga tggcaaggat cggcgaatct tcattgttga atttagagat
    11161 gaccggagga tcgacttcgc gcggcagacg attgagaacc gtggcgaccc ggtcgcggac
    11221 atcctgagca gcgacatcga tgtcgcggtc gagattgaac gtggcgatca cgaacgaggt
    11281 tccgcgcccg gacacggaac gcagctcatc gattccttcg acggtattga cgacctcttc
    11341 gatgggttgt gaaattacgg tctcgacctc ctcaggggaa gcgccgggca gagtagtgcg
    11401 caccgaaacg ttgggcaggt ctacggaggg gaagcgatcg acacccaatc ggaaatagct
    11461 cgccgcgccg acgaccacca gcgccaaaac gatcatcgag gcaaacaccg gacggcggat
    11521 cgatacttcg gcgagcttct gcatcagttc attactttca cgggctgccc ggcaaccaga
    11581 tttcccgggt cgacaatgac ttgatagccg gccacaacgc cttcgacaat ctcgatctga
    11641 tcgccgatac ggcgaccggt gcgcacccgt ttttccaccg cttgcccctc ttccacactc
    11701 aagaccttat cgattccagc gaacgtgatg atcgcatcgc gcggcaccgt aacgatttgc
    11761 tcgccggctt ccacgataat atcggctttg gcgaatgatc caggacgcaa gcggccgttg
    11821 ctattggcca cctccgcctc aatcagcaat gttcggttgt tctcgctgat cgccggagat
    11881 aaccggacga cccgaccgga atagcgctgg ctatcacctt ccaccataag ctcgaccgtt
    11941 tggccgacgt gcacggatcc cgcctgccgc tcggggaccg ccagccgcaa acgcaatgga
    12001 tccatgcgca ccagcgtgac caccggcgca ccggcagtca aatactggcc caccgagcct
    12061 tggcgctcgc taaccgcgcc gtccatcggc gacacaataa cggtgtcggc gagctgctgg
    12121 cgcgcgatct cgagctccga ccggcgttga aacagaaggc cctggcggat tctgacttct
    12181 tcgagcgcgt tctggtactg gctttcggca atcgccaatt gcgccaccgc agcatcgagc
    12241 tcagcgcgcg cgatataatt accttcccac aatttcgcca tgcgatcatg ggtcaggcga
    12301 gcttctttga gagtagctgc cgcttgacgc acggtcgcgg tttgctcggg ttcgatctta
    12361 tcgctggtgc cttcggccgg cagcccgaga cgaactcggg cttgctgaag ggcagcctcg
    12421 gcttggtcca cccgcagccg gtaatcactg ggatcaatgc gcgcgatcgc ctggcctttg
    12481 cgcacccggc tgccgagatc caccgagatc tcgccgagcc ggccaacgac tttggtgccc
    12541 aggacaactt gatcttcggc tgctagcgtc ccggtggcgc tgaccgttcg ggccactttg
    12601 gcctccacgg ccgtggcgat ttttacctgg cgcggcgccg gcgaactttc gatcactgca
    12661 gtggctgatt gatcgccgcc acatcccgcc agcgcgagcg gcagcagtag agccgcggat
    12721 agcaggccgc gccggcgctg cccggttgat cgaagccaag tcatcgatca atccttcgaa
    12781 cgcgcttggt ccgcactggg tgtgtcctcg ccggttgtag agcgcggcga ctcttcgcac
    12841 tcgacccggc gccatttcct tgggcaaaac gctgcaacgc gccctgagaa aatcgggtaa
    12901 tatgatcagc cagcaggacg atgtccgggg agccgtattc ctgttccgga taaagacggc
    12961 tcaggaccga gcgggcatga tggtagtaaa cacattggct taaaatactc agcgtgcaga
    13021 gccgcacggc atcttcgctg gctctcgatc ctaacagctc gcggacgatg gacgccagct
    13081 cttgatgcac cggcaaggca acatcggcta ccaaagtatc caaagcacga gtaggctcaa
    13141 tcatttcccg agccataatt ttggtatgcc atccgggccg tccttcatcg aaaactcgat
    13201 ttaacaagga ttcgatatag gctgcgagcc tctgttcggc ggttgctcca ggagataggc
    13261 cccggtcggc aggaaatttc tccgcatgag catccggcac ggagcgcagc actgccatgt
    13321 agagtccctc tttgtcgccg aagtggtagt tcacggcggc aatgttggcg ccggccttct
    13381 cacagatttg ccgtaccgta gcggcgcgat aaccgacttc cgcgaaaatc tcgcccgcgg
    13441 cttcgagcag acgacgctcg gtggcggaag acaattccgt cgaatgttta tcctgatgat
    13501 taaacatatg tttcaagcaa ccgtatcaaa cggccgttgg gcgcacaagc catataggca
    13561 ggctcggtgc gccgctcgct tccatttgct tcaaagtgcg tcagatgcta gacgaaactc
    13621 atcgtagtca ggaggatgat catggcaaaa ctcagacatt tagcgttgtt gacccatcaa
    13681 ccggagaagc tcgccgattt ttataaaaaa gttttcgagc tcgaagaagt gtttcgcacc
    13741 aagaatggct ccgtacacct gtcggatggc gaagtgaatc tggcgatctt gaacgccaat
    13801 gatcccaagg atgcggacga tctaaaacac atgggcatgt accacttcgg ctttcatgtc
    13861 gatgaccagg agacggttgc aaatagaatt caggaaatct atccgcaagg agcgccaaag
    13921 aagcggccca acggcaccag ctacgctgaa accagaggga cggatcccga cggcaacatc
    13981 tttgacatct cgacctgggg gtggagcgga aagccgctgc cgaaagacaa aggcggaccc
    14041 gaacaacagt aaccaaggga gttaaccatc agatgacgaa gttcctgctg ggattattgc
    14101 tgatactcgc cacgttttcg attcactcaa acagcggcgc cgccaccgtt gccgacgtcc
    14161 tgaagaaaat acagcctctt caaccgccgc agagaaaagc tgcgttggag gaaggcgcca
    14221 aaaaagaagg tcaggtcgtc atctatacgt cggtgagctt atcggattat ccgaaaattc
    14281 tgtccgcttt tgaacaagcg catccattta tcaaaactca cgcgttccgc tcgacaccct
    14341 cgggtgtatt ccgccgggtc gataccgaag ccaagaccgg ccggttcgcg gtggatctgg
    14401 tcggatcggc gccgatagaa gtttggcaac tgaaggagcg gcaactctcg actccctatg
    14461 cttctcccga acgaggcaat ttattcaaag gagcattcga tgccgacagc tactggacgg
    14521 ctttcgacat cacgccgatc gtgctcgcct acaataccaa gctggtagcc gcgccggagg
    14581 tgcccaagaa ttatcaagat cttttgaatc ccaaatggcg cggcaagatg agccttggca
    14641 cggaggatta cgattggttc tcggtcatgt tggaccacat gggcaaagaa aaggggctcg
    14701 aatacatgaa cagcctggcg aagcagaatt tgcatatgcc gggctcgagt agcgtcatga
    14761 gagtccagtt gttgctcgcc ggcgaatcgt cgatcgccat agccgcacga ggccgtagag
    14821 ttgcggagct caaatccaaa ggcgcgccca tcgatttccg aattttcgat ccttatgccg
    14881 gcgaacccga tttgctggca ctgctgcagc gcgcccccaa tcctcatgcc gcaattctct
    14941 tttacgactg gctgctctcc caggacgggc agaccaagat gtccgaccag accggccgca
    15001 tttcggttag gaaaggaatc aagcaccagc cctggcttca ggaactactg cagaaggatt
    15061 ttgttttcgt cacgccaacg tcgagagccg tgaacttaaa agaagtgacc gagctctacc
    15121 atcaggtatt cgggctgcat caggcgcgct aagaagctca agatggtgat cggtccgttt
    15181 caatagttca aaacgttcaa ccgcttcgct ccgttaaaaa cgtcactcga agaaagccgg
    15241 ttcaaggcta gttacttaat ctcgacctgc gccgcgcacc aggaaagcat ttcgatctgc
    15301 ggcatggttt ccacgatgga tccggctcgc gacaccgcgc tttccgcttc gtcaaacggt
    15361 tgccctaacg caagcagcac cgagaccgca agcatggcgg tgcggccgac gccgccagcg
    15421 caatgaatca gcagcgcttc cccggattgc aatcgctctg ctagatccct ggcaagctgc
    15481 cagaacgcct gacgatccgc cggcacaccg ccttcacgaa tttcaaaggg cagcacggaa
    15541 cagggaactg ccccggcttc caaagcgtca gcatagttag aagatttcag gcggatttcg
    15601 tacttttccg tgagacagac aatcgcgcgc accgcatcga tctgcacctg ccgccaaacc
    15661 ctctcgatcg cctcaaaacg gcccggcatg ctgtgcaaca gcagtgttcc aggaatccgc
    15721 gcgggaagat cgacacgacg aaacatctag aatctcctag gaagcagcca tcgatgttac
    15781 tggctagaaa tattttgtaa tcgtcatcgc acgccgagat gatgatgtaa agtcggcaac
    15841 cctttgaaca gagcgagcgg ttgaacccat cggaacattt ggaccggctt caatggtgag
    15901 attgacacct gatttttgaa tccgctagat tggcgtgcca cctaaaatga aaaagatttt
    15961 ctgcttttta ttgatcgcgc tcttctcttt tctctcggaa aatagttttg cgcacttcac
    16021 cggcaaaggg catgttcaca ccctctcgga aacgaaacag gattatttga attccgattg
    16081 tcggccgacc gcctcctgcg atctcaaaag attcacgtta acaacatccg tttacgaagt
    16141 ctggttcagt gacgatccaa actatcccac ttatggcaac agcgtgatta tggagtatca
    16201 gaccgactcg gtagctgcac tcgagagata cgccatcgtt caattcaaaa aaggttgtgt
    16261 attttacact tcaaaaaccc ccgccggaaa catcgatacg aaagtaaatg atacggtgcc
    16321 gagctttggc gaagatgttc ccttttgctt tccgcgctgg gtaatcgatt cacaggacac
    16381 cgatcccgct tacaacagcg atccggaaac cggcagattc tatttactac ggtggaataa
    16441 accaggg
//
LOCUS       DASUVS010000212        10342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_305799, whole genome shotgun sequence.
ACCESSION   DASUVS010000212 DASUVS010000000
VERSION     DASUVS010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10342
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305799"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..762
                     /locus_tag="VF182_11970"
     CDS             247..762
                     /locus_tag="VF182_11970"
                     /inference="COORDINATES: protein motif:HMM:NF017830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydratase"
                     /protein_id="HEX6437842.1"
                     /translation="MKQLIPIDLTRVVDEVKAEGRGRRVLWQDSESLAFLSSGRKERK
                     DFHVDPGDEVTVQLSGVQNLVYIDNEGKQQTAVIKAGQMLLCPGGVPHSPRVAENSWF
                     IVFERKRKSGERDHFIWYCDKCGEKIYEVAVEVGDYRQDPVSQVHQRFYNDETLRTCA
                     KCGAIMPVPPP"
     gene            778..1557
                     /locus_tag="VF182_11975"
     CDS             778..1557
                     /locus_tag="VF182_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437843.1"
                     /translation="MANRWAQQLTKLASAYGAAEAEVVGAFFTVPRQKQDHLRWLKAQ
                     GFKEYSAIKPILDALTQLYPNIDNGIARHDYTELTEKLADETKHARLVMDLLEEISGK
                     KIKPRDLTWLPQDRKLARVRAGYSKTYAGLLHGSAKVTSQEMRRKDEELERAAITLTE
                     GGGGALYQVCGKLKKRGIEEKIAAVFQEILQDELEHKDGGSRSLATLVKDQASFRRAA
                     QIICEVSRQRLRMRNEQFGFPLSEAQMKTLDEHARQSAGGL"
     gene            1557..2429
                     /locus_tag="VF182_11980"
     CDS             1557..2429
                     /locus_tag="VF182_11980"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX6437844.1"
                     /translation="MLLLFLVAFFLSLSWHSPSSALELPPLNDTINDLAGIMPEPSYL
                     DLEKRIKRFKARSGHSVVVITLQSLEGEDPESFGEKAFHSLPLGEADLKKTILLTVAR
                     KEHAVSLQTGAELKELFPEPTAREKLQKHVDVYFNGMRPDLGIHSAVFFVFRTIRGEV
                     KMDHATDIEKLEEASTQGAEAGPIFAIFLAPFLAFFIGGLWGICSTNYGVQTAVRLTT
                     GAVLGGGTAKIVAGLLELMGAVGDGLWYFILFLSIALATFGSLTEFWMAGDWRGIPRV
                     KDPVVRKPEDNIGI"
     gene            2608..3591
                     /locus_tag="VF182_11985"
     CDS             2608..3591
                     /locus_tag="VF182_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6437845.1"
                     /translation="MTLLAPTDKQTAQPDVIERRSISRRELLRLALGAGISVSTGGMA
                     MAAATSSKAILTKPIPRTGESLPVIGLGTWQTFDVGSAQSERAPLRQVLGEFVRFGGT
                     VIDSSPMYGRSENVAGDLANELKLDNKLFVATKVWTSGREAGIRQMEESFRRLRIKRI
                     DLMQIHNLVDWRTHLTTLRRWKKEKRVRYIGVTHYTASAYDQLARVLESEELDFVQLN
                     YSIVEREAEQRLLPLAADRGMGVLVNRPFAQAQLFSKVRGKPLPAFAAEIDCASWAQF
                     FLKFVISHPAVTCAIPATSKVQHLTDNMQAATGQLPATEIRQRMAKYVADL"
     gene            4040..5719
                     /locus_tag="VF182_11990"
     CDS             4040..5719
                     /locus_tag="VF182_11990"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX6437846.1"
                     /translation="MNDIFISYDSADRPIAQKFADALETRGWSVWWDREIPLGKAFDQ
                     VIEEELNGARCVIVLWSKESVRSRWVKTEAAAAADRDCLLPVLIEDIAIPFEFKRIQT
                     AMLINWRGDDSDPEFNRLVSAIEQLAKAPPRPLPSRPAVAIPAPWPKPTNRWIIPIAL
                     IALVIAGTLALKNFARRSDPPPQQVSSESAKPSATPTQSTRSPASFPEPSAGTPKLTA
                     PQGAFPIKIGDKIADGVPAAGAGIIETPYEQDVYVFKAAARQKVYFRMLGRGTGMDYI
                     NWKLTDSDGAEVFNTCLGCGEVGVRSLTKGGTYTLTVGNDKQRATGTYQLQLIDVPPP
                     NQFSIKIGDQIKDDVPGPGAGSIESPGAEDIYTFTAAPRQKVYFRSWEHSTGMSYIKW
                     QLIDNDGMQVFDSCLGCGEPGVQTLTKGGSYTLTVGHQNNPATGTYRVQLYDVPKAKQ
                     FSINIGDKIRPGIPAAGAGTIESPGSEDVYLFSATPGQTASFRITERSKGMDYINWRL
                     VDDNGMELFNSCLGCGERGVQTLSKGGTYSLTIGNTRNPATGDYGFEIGSR"
     gene            6066..9707
                     /locus_tag="VF182_11995"
     CDS             6066..9707
                     /locus_tag="VF182_11995"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX6437847.1"
                     /translation="MRSMTDLTPKIRIVGIGASAGGLESLKTFFTEVPADSGFAFVII
                     QHLDPNRESLMAELLSKHTKMAVTAARDQVAVEANHVYTIPPNKFLFVKDAALHLADP
                     FSNDGARMPIDFFLRSLASDQEASSIAVLFSGGGSDGTLGIREIHGAGGLVIVQDPET
                     AQHQSMVENAIATGLVDYVLPAREIPAKLIDYVRHRPSIDTSDTDSSTGAVDGVLNLL
                     ATDNKHDFRAYKKNTVRRRIERRMGIHHISELSEYYDFVRGNSEELAKLAKDLIISVT
                     SFFRDPEAFKELLDKVIAPLVQEHDPKEPLRVWIPGCATGEEAYSIVILLLEEMARAS
                     KNTTLQVFASDIDREALKRARHGIYPESISVDISPERLATFFIKQDGAYRVDQRVRDC
                     VTFAEHNLILDPPFMRLDLVSCRNLLIYIEPAVQEKIVNLLAFALKPSRYLFLGKADT
                     PADRSEFFEAVSRTWRMFRRRKSLALPLGRVPPGVLSRQVRDEEPPPRRLGELSQDVL
                     LQHFQAAMVMVNERGEILHFHGRTEKYLTHPVGDANLSLYDMIDRQYSLALRLGAERA
                     GRENRQIELKLRQFNRQDNDEPIAVTISPVSDPRSGKRLLAVIFEAAAAAPLKEPVLP
                     PRPVEQQDFDVFNQLQAENDRLKHDLQSAIENFQVTHEEFTAANEEVLAINEELQSTN
                     EELETSKEELQSVNEEMITVNAQLNDKVEELTTAHDDLANLVNTSEVGTLFLDRACCI
                     RRFTPSMTRILNLLPLDVGRPVNHIATRMIGVDLVAIAEAVLQSLSPYQQEVLTADRL
                     WYMLYCVPYRTQNNVIDGLVFTFTDVTRLKHSEQVMRQARTFAENIIQTVSESLLVLD
                     SELKIVSANQAFYRTFHVGPEDVEGRLIYNLGNRQWNIPKLRELLEGVMHRDGSFENF
                     EVEHEFQDIGRKIMSLNCRKFSASQSDEARLILLAIDDITERRQAEAERSWLEGELRQ
                     AQKMESLGTLAAGIAHDFNNILNIVQGYAFVLRDSKIADELVKESVAAILDSTTRGAT
                     IVQQLLTLARKTEPKFDSLDIDALIEEVAQLFGKSSPHTIEIKLELSSPTPPILADRN
                     QIFQALLNLCLNARDAMPNGGTLKLKTSLANCNAVRNTVAGSEEVTAEQYVRIDVDDT
                     GEGIDENVLGRIFEPFFTTKGAGRGTGLGLAVVYGILKHHKGFINVTSKPGAGTSFQI
                     YLPVTRRSD"
     gene            9721..10128
                     /locus_tag="VF182_12000"
     CDS             9721..10128
                     /locus_tag="VF182_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6437848.1"
                     /translation="MEPGNNHSRTILVVEDEGLMRRLLQRILCTDDTSVLLAADGQEA
                     VDLYRDHKLEISVVLLDLGLPKLSGWEVFKNLKQQNPDVCVIVASGYLDPDVKTRMHD
                     AGVQYFIEKPYKLNELLATLHGVINYGCEWPNG"
     gene            10148..>10342
                     /locus_tag="VF182_12005"
     CDS             10148..>10342
                     /locus_tag="VF182_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012413351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX6437849.1"
                     /translation="MANRDIIVMGASAGGLQALTRIVKDLPEHLKAAVLIVWHVSPYA
                     HSILPEILSSAGKLKAHHAVD"
BASE COUNT         2393 a         2896 c         2906 g         2147 t
ORIGIN      
        1 gaattttcca atcacgtttg gtcacttgaa gaactggcgg tctggcaaac tgacacacta
       61 gttaaaggcc gcctaaggca agagctaaga catagccgga taccggaaga atttgcgtcc
      121 actgtttgcc cccggtgggg cgggctcaag taatcgacgc cgttatccgg agacaaccct
      181 tcgatatctc ctcagcaaat gccaatgctg ttgaacctgc gctttcgata gcgctatagt
      241 cggctcatga aacagctcat tcccatcgat ctcactcgtg tcgttgacga ggtcaaggcc
      301 gaaggcaggg ggcggcgcgt cttgtggcag gattccgaat cgctggcgtt tctcagcagc
      361 ggtcgcaagg agcgcaaaga tttccatgtc gatcccggcg atgaggtcac ggtgcagctg
      421 agcggcgtgc aaaatcttgt ctatatcgac aacgaaggca agcagcagac cgccgtcatc
      481 aaagccggcc agatgctgct ttgtccgggc ggcgtgccgc actcgccccg ggtcgcagag
      541 aactcctggt tcatcgtttt tgagcgcaag cgcaaaagcg gcgaacggga tcattttatc
      601 tggtactgcg acaagtgcgg cgaaaagatt tacgaggtcg ccgtcgaagt cggcgattac
      661 cggcaggatc cggtctctca agtgcatcaa agattctaca atgacgaaac cctgaggacg
      721 tgcgcaaagt gcggcgctat catgccggtg ccgcccccgt gagagcgaca aatttctatg
      781 gcaaatcgat gggcgcaaca gctgaccaag ttagcatcgg cctatggcgc ggcggaagcc
      841 gaggtcgtcg gcgcgttttt taccgtgccg agacagaaac aagaccacct gcgctggctc
      901 aaagcgcaag gtttcaaaga gtactcggcg atcaagccga tcttggatgc cttgacccaa
      961 ctctatccga atatcgataa tggcatcgcg cgccacgact acactgaatt gacggaaaaa
     1021 ctcgccgacg agaccaagca cgcgcgtctg gtgatggacc tcctcgagga aatcagcggc
     1081 aaaaaaatca agccgcgcga tctcacgtgg ctgccgcaag accgcaaact cgccagggtc
     1141 cgcgccggct attcgaagac ctatgccgga cttttgcatg gatcagcgaa ggtgacgtca
     1201 caggagatgc gccgcaaaga cgaagaactg gaacgcgccg ccatcaccct gaccgaaggc
     1261 gggggcggcg cgttgtatca agtgtgcggc aaactaaaga agcgcggcat cgaagaaaaa
     1321 atcgccgcgg tctttcaaga aattctccaa gatgagctgg aacacaaaga cggcggcagc
     1381 cgctcattgg ctaccctggt caaagatcag gcctcatttc ggcgcgccgc acagatcatc
     1441 tgcgaagtct cgcgccaacg gctgcggatg cgcaatgaac aattcggctt tccactcagc
     1501 gaagcgcaga tgaagacgct ggacgaacac gcccggcaat cggcgggcgg tctgtgatgc
     1561 ttctgctctt tttagtcgcc ttcttcttaa gcttgtcttg gcactctccg tcaagcgccc
     1621 tcgagctgcc gccgctgaac gataccatca atgatcttgc cggcatcatg cccgagccca
     1681 gctatctaga tctcgaaaag cgcatcaagc gtttcaaagc gcgcagcggc cacagcgtcg
     1741 tcgtcattac tctacagagt ctggaaggcg aagaccctga aagcttcggt gaaaaggctt
     1801 ttcacagctt gcctctcgga gaagcggatc taaaaaaaac gatcttgctg actgtcgctc
     1861 gcaaggaaca tgcggtcagc ctgcaaaccg gcgcggagct gaaagaattg tttccggaac
     1921 cgacggcgcg agagaaactg cagaagcacg tcgatgtgta tttcaacggc atgcgccctg
     1981 atttgggaat tcattcggcg gttttctttg tattcaggac gatccgagga gaagtcaaaa
     2041 tggatcacgc caccgacatc gagaagctcg aggaggcgtc gactcagggc gccgaggcgg
     2101 gaccgatctt cgcgattttc ttagcgccgt ttttggcttt ctttatcggc gggctttggg
     2161 gaatctgctc gaccaactat ggggttcaaa ctgccgtgcg actgacgacc ggcgccgtgc
     2221 ttggcggcgg taccgccaag atcgtcgcgg gtttattgga actcatgggc gcagtgggtg
     2281 atggcttatg gtatttcatt cttttcctaa gcatagctct ggcaactttc ggcagcctca
     2341 ccgagttctg gatggcgggc gactggcgcg gcataccgag ggtgaaggat ccggtggtac
     2401 gcaaaccaga ggataacatc ggaatctgaa atgaagcgcc acgcaacaag ctgccaggta
     2461 tcagaacata gtgttgagtt ttacgccaaa gccgtcaccc ccgaatgtgt aattgggctc
     2521 caactccgtt ctcattcccg ataaaagcat ggggaatgac gactcgataa tttctgaggc
     2581 gacctaaagc tctatcggtg atgcgcagtg actttactcg ccccaaccga caagcaaacg
     2641 gcgcagcccg acgtcataga gcggcgatcc atttcccggc gcgagttgct gcggctggca
     2701 ctcggagccg gaatatctgt cagcacggga ggtatggcga tggcggcagc cacttcatct
     2761 aaggcgattt taaccaagcc cattccgcgc accggcgagt cgctgccggt gatcggtctc
     2821 ggcacctggc aaacttttga cgtcgggtcg gcgcaatccg agcgcgcgcc tttgcgccag
     2881 gtattgggcg agtttgtccg gttcggcggc acggtgatcg actcctcgcc gatgtacgga
     2941 agatccgaaa acgttgccgg agatcttgcc aacgaactga agctcgacaa taaactcttt
     3001 gtcgccacta aagtttggac gagcggccgc gaggccggca tccgccagat ggaagaatcc
     3061 tttcggcgcc tgcgcatcaa acgtattgac ctgatgcaaa ttcacaatct ggtcgattgg
     3121 cgcacgcacc tgaccactct gcgccgctgg aaaaaagaaa aaagagttcg ctacatcggc
     3181 gtcacgcatt acacggccag cgcttacgac cagctcgctc gtgtgctcga aagcgaggag
     3241 ctcgatttcg tgcagctgaa ttattcgatt gtcgagcggg aagccgagca acggctgctg
     3301 ccgctggcgg ccgaccgagg tatgggagtg ctggtcaacc ggccgtttgc ccaagcgcaa
     3361 ctgtttagca aagtcagagg caagccgctg ccggcctttg ctgcggaaat cgattgcgca
     3421 agctgggcgc aattttttct caagtttgtg atctcccatc cggccgtcac atgcgcgata
     3481 ccggcaacca gcaaagttca acatctaacc gataatatgc aggccgccac gggccagctt
     3541 cccgctacag aaattcgcca gcgcatggcg aagtacgttg ccgatctatg atccggcgtg
     3601 actagcatac gactaaccca cgtatcgcta tcttcgtcgg cctgacagat tccaagctga
     3661 ctgcgccggc aaaaacgctt tacaacatac cccaccgcag agtaagtttc tcaccggcgc
     3721 cggcgttcga tcgcaccggc cagatctcta atccactcga gcaaattgtc ccgggttccg
     3781 cagtagggat taaggatgtc ttctgatcga ggggcgaagc ccggaggagc gaaggcacag
     3841 aagcgaacaa tttcaatgcc gtctagggtg cttggtccag tgctcgtagt ctgtcaagga
     3901 agtccactgc accaccggcg tttacaggcc gcgccaagac actggcccat tgcatcgcgc
     3961 cggagggctc gccacttgat cgccgcgtca tttgaactgc ggaatccggg gatcattaaa
     4021 atcacttcgc tgtaccttca tgaatgatat tttcatcagc tacgacagcg ccgatcggcc
     4081 gatcgcgcaa aaatttgccg atgctttgga aacgcgcggc tggtcggtct ggtgggaccg
     4141 ggagatcccg ctaggtaagg ctttcgatca ggtgattgag gaagagctga atggggcgcg
     4201 ctgcgtgatc gtcttgtggt cgaaagaatc ggtacgctcg agatgggtga agaccgaagc
     4261 ggcggcggcc gcggatcgag attgtctcct gcctgtgttg attgaggaca tcgccattcc
     4321 gttcgagttc aaacgcattc aaacggcgat gctcatcaac tggcgcggcg acgattccga
     4381 tcccgagttc aaccggctgg ttagcgccat cgagcagctg gctaaggcgc cgccacgccc
     4441 gcttccctcg cggcccgcag tagcgatccc ggcgccgtgg ccaaagccaa ccaaccgttg
     4501 gatcattcca atcgcgctta tagcgctggt gatcgcgggc actttggcgc tgaaaaattt
     4561 tgctcgacga tctgatccgc cgccgcagca agtctcgtcg gaatccgcca aaccttcagc
     4621 gacgccgacg cagtcgacca ggtcaccggc atcctttcct gagccctccg ccggcacacc
     4681 gaaattaacc gcgccgcaag gcgcttttcc gatcaagatc ggagataaga tcgccgacgg
     4741 cgttccggca gccggcgcgg gcatcatcga aacgccatac gaacaggatg tctatgtgtt
     4801 taaggctgct gcccggcaga aagtttattt ccgcatgctc ggccgcggca ccggaatgga
     4861 ctacatcaac tggaaattga ccgattcaga cggcgccgaa gtattcaaca cctgcctggg
     4921 ttgcggcgaa gtcggcgtgc gctcgctgac aaagggcggc acttacacgc tcaccgtcgg
     4981 caacgacaaa cagcgcgcga cggggaccta tcagctgcag ctaatcgatg tgccgccgcc
     5041 gaatcagttc tcgataaaaa tcggcgacca gatcaaagat gatgtgcccg gccccggggc
     5101 tggaagcata gaaagtcccg gagcggaaga tatctacacg tttaccgcgg caccgcggca
     5161 gaaagtttac ttccgatctt gggagcacag caccggcatg tcgtatatca aatggcagtt
     5221 gatcgataac gacggcatgc aggtgttcga ctcctgtttg ggctgcggcg agcccggcgt
     5281 tcaaactttg accaagggcg gtagttatac cctcacagtc ggccatcaga ataatcccgc
     5341 caccggtact tatcgggtgc agctttatga tgtgcccaag gcgaaacaat tctcgatcaa
     5401 catcggcgat aagatcaggc ccggcattcc agccgccgga gcgggtacga ttgagagtcc
     5461 gggctccgag gacgtttatt tattcagcgc cacgccgggc caaacggcgt cctttcggat
     5521 aaccgagcgc agcaaaggga tggattacat caactggcgc ttagtcgatg acaacggcat
     5581 ggagctgttt aacagttgtc ttggttgcgg ggagcgcggc gtgcagacct tgagtaaagg
     5641 cggcacttac agcttgacga tcggcaatac tcgcaaccct gccaccggcg attacggatt
     5701 tgaaattggt tcccgttgaa actcgggtga aaaaattctg ttgtatagga gcggtatact
     5761 aaccgccgga gccgccccat acggcaaaga cggcggctct tcagcgcgcc gcatgaaacg
     5821 ctgaacctcc tgcgagatca tggatcttgg cttacgcacc gctgcagcgg tgggactgag
     5881 aagcaatgga agtcccaatg ctcctaaaat tgtttgactg catctggcgg ggatcttaga
     5941 aagcggagcg cgccggcccc tgtttcaatt ccagattcgg tcgtcgcatc gagcgagaat
     6001 tccatgccaa aaaagtttta ctggcaaacc tacttgatcg ttcttcatcg ggcgatagga
     6061 tggctatgag atctatgact gacctgacgc caaaaatccg catcgttggt atcggcgctt
     6121 ccgccggcgg actcgaatcg ttaaaaacat tctttaccga agtgccggcg gactccggtt
     6181 ttgccttcgt gatcattcag catctcgacc cgaatcgcga aagcctcatg gccgagttgc
     6241 tgtcgaagca cacaaagatg gcagtgacag cggcacgtga tcaggtggcg gtggaagcca
     6301 accatgttta caccattccg cccaacaagt ttctgttcgt caaagacgcc gctctccatc
     6361 tcgccgaccc gttctcgaac gacggcgcgc gaatgccgat cgatttcttt ctccgttccc
     6421 tcgcttcgga tcaggaggca tcgtcgattg ccgtgctttt ctccggcggc ggttcggacg
     6481 ggactctcgg cattcgggag atccatggcg ccggcggact ggtgatcgtc caggaccccg
     6541 agacagcgca gcatcagtcg atggtggaaa acgccatcgc cacgggtcta gtggattatg
     6601 tcctccccgc gcgggaaatt ccggccaaat tgattgatta cgtgcggcat cggccatcga
     6661 tcgatacctc cgataccgac tcatcgactg gcgctgtcga tggcgttctg aaccttttgg
     6721 cgaccgataa caagcacgac tttcgcgcgt acaagaaaaa caccgtccgg cgccggatcg
     6781 agcgccgtat gggcatccat cacatttctg aattatccga atattacgac tttgtgcgcg
     6841 ggaattccga ggaactggcg aagctcgcca aagatctgat catcagcgtc accagtttct
     6901 tccgagatcc cgaagctttc aaagaattgc tcgacaaggt catcgctccg ctggttcaag
     6961 agcatgaccc aaaagaaccg ctacgggtat ggattcccgg ctgcgccacc ggcgaagagg
     7021 cctattccat cgtcattttg ttgcttgagg agatggcgcg ggcgagcaag aatacaaccc
     7081 ttcaggtttt tgcttcggat atcgatcgcg aggcactcaa acgcgcgcgg cacggaattt
     7141 accctgaaag catcagcgtc gacatatcac cggagcggct cgcaactttt tttatcaaac
     7201 aggacggcgc gtaccgggtg gatcaacggg tgcgcgattg cgttaccttt gccgagcata
     7261 acctgattct ggatccaccg ttcatgcggc tcgatctggt cagctgccgc aatctgctga
     7321 tttatatcga accggcagtt caagagaaaa tcgtgaacct gttggccttt gctctgaagc
     7381 ccagccgtta tctgttcctc ggcaaggcgg acacccccgc ggataggagt gagttcttcg
     7441 aagcggtgtc gagaacttgg cggatgttcc gccggcgaaa gtccttagca ttacccctgg
     7501 gccgagttcc ccccggagtg ctcagccggc aagtcagaga cgaggaaccg ccgccaagga
     7561 gactcgggga gcttagccag gacgtgctgc tgcagcactt ccaggcggcg atggtcatgg
     7621 taaacgagcg aggcgagatt ctgcattttc atggccgcac cgaaaaatat ctgacacatc
     7681 ctgtgggcga cgccaacttg agcctgtatg acatgatcga cagacaatac tccctggcgc
     7741 tgcgcctggg tgccgagcgc gccggccggg agaatcggca gattgaattg aaactgcgcc
     7801 agttcaatcg ccaggataac gatgagccga tcgcagtcac catctcaccg gtcagcgatc
     7861 cgcgctcagg caagcggctg ctcgcggtga ttttcgaggc tgcggcggcg gcgccgctga
     7921 aggagcctgt tttaccgccg cggccggtgg aacagcagga tttcgacgtt ttcaaccagc
     7981 ttcaggccga gaacgataga ctcaaacacg atctgcaaag cgcaattgag aatttccagg
     8041 taacgcacga agagttcacg gccgccaatg aagaagtgct ggcgatcaac gaggagctgc
     8101 aatctaccaa cgaagaactg gaaacatcaa aggaagaact gcaatcggtc aacgaagaaa
     8161 tgatcaccgt taacgcccag ctcaacgaca aggtggaaga gttgaccacc gctcatgatg
     8221 atttggccaa tctcgtcaat acatccgaag tgggcacgct gtttctcgac cgagcctgct
     8281 gtatccgtcg tttcaccccc tccatgaccc gaatattgaa tctgctcccg ttggacgtcg
     8341 gccgtccggt gaatcacatc gctacgcgga tgatcggcgt cgatctcgtt gccatcgccg
     8401 aagccgttct ccagagtctt tctccttacc agcaggaagt gctgaccgcg gaccgtctct
     8461 ggtacatgct ctactgcgtt ccctatcgaa ctcagaacaa cgtcatcgac gggctggtct
     8521 ttacctttac cgacgtcacc cggttaaagc attcggaaca ggtgatgcgc caggcgcgca
     8581 ccttcgccga aaatatcatt caaaccgtta gcgaatcgtt gctggtgctg gattcggagc
     8641 tgaagatcgt ctcggccaac caggcattct acagaacctt tcacgttggg ccggaagacg
     8701 tcgagggacg cctgatttac aatctcggca accggcaatg gaatattccc aagttgcgcg
     8761 aattgctcga aggagtcatg caccgagacg gcagcttcga gaatttcgag gtggagcacg
     8821 agtttcaaga tatcgggcgc aaaatcatgt cgctcaactg tagaaagttc tccgccagcc
     8881 agagcgacga ggcgcgcctg atcctgctgg caatcgacga catcaccgag cggcggcaag
     8941 ccgaagccga acgcagttgg ctcgaagggg agctgcgtca agcgcagaag atggaaagcc
     9001 ttggcaccct ggcggcgggg atcgcgcacg acttcaacaa cattttaaat atcgtgcagg
     9061 gatacgcttt cgtcttgcgg gattccaaga tcgccgatga attggttaag gaaagtgttg
     9121 cggcgattct tgacagcacc acacgcggcg cgaccatcgt tcagcagcta ttaacattag
     9181 cgcgcaagac agaacctaag ttcgactcgt tagatatcga tgccttgatc gaggaggtgg
     9241 cgcagttgtt cggcaagtcc tctcctcaca caatcgaaat caagctcgaa ttaagctcgc
     9301 cgaccccgcc gattttggcc gatcgcaatc agatttttca agctctgtta aatctttgct
     9361 tgaacgcgcg cgacgcgatg cccaatggcg gaaccctcaa attgaaaacg tcgctggcta
     9421 attgtaatgc cgtgcgtaat accgtggctg gctcggagga ggtcaccgcc gaacagtatg
     9481 tgcgcatcga cgtcgacgat accggcgagg gaatcgatga aaacgttctg ggtcgaattt
     9541 tcgagccttt cttcaccacc aaaggcgccg gccggggaac cggtctgggt ctggcggtcg
     9601 tatacggaat tctcaagcac cacaaaggct tcatcaacgt aacaagcaag cccggcgcgg
     9661 gaacgtcttt tcaaatctat ttaccagtta cgaggcgaag cgattgaagg attgaccgga
     9721 atggaacctg gcaataacca ctcgcgcacc attcttgtgg tcgaagacga aggtctcatg
     9781 cgccgcttgc ttcaaaggat tctctgtaca gatgatacgt cggtgctgct ggccgctgat
     9841 ggtcaggaag cggtggacct ctatcgcgat cacaaactgg agatcagcgt ggtcttactg
     9901 gatctcggcc tgcccaagct ctcgggttgg gaagtgttca agaatttgaa acagcaaaac
     9961 cccgacgtct gcgtgatcgt cgccagcggt tatctcgacc cggacgtcaa gaccaggatg
    10021 cacgatgccg gcgtgcagta tttcattgaa aaaccgtaca agcttaatga attactagcg
    10081 accttacacg gggtcattaa ctacggatgt gagtggccta atggctagcc tctagaaggg
    10141 agtcttcgtg gcaaaccgcg atatcattgt catgggagct tccgccggtg ggctgcaggc
    10201 gcttacccgg attgtcaaag accttcccga acatctgaag gcggcggttc tcatcgtctg
    10261 gcatgtctcg ccctatgccc attcgattct gcctgagatc ctcagttcgg cgggtaaact
    10321 caaggctcac catgcagtcg ac
//
LOCUS       DASUVS010000213        16912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_306531, whole genome shotgun sequence.
ACCESSION   DASUVS010000213 DASUVS010000000
VERSION     DASUVS010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 16912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16912
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306531"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..1448
                     /locus_tag="VF182_12010"
     CDS             372..1448
                     /locus_tag="VF182_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437850.1"
                     /translation="MSRFGSKYTLALAFFFLVSAVLTTIFINPVFSQAPFYQGKTITL
                     IIGAGPGGMGDLRARALASVLVKHIPGNPTITFQYMAGAGGRKAANHLYNTARADGLT
                     LFRISSSIIPYAVLGESGVQYDIDKLVYLGTTEHILYYLFNTRKQLGLDTLAKLRAAS
                     GVRIGSAPVGHTGYIQSRVTAYLLDMKEPRFIPGYENEDLDVAFARGEVDAQVASTST
                     VIEHDLLNKKLVDFHAAIEVPKGRKDSRFAKLNLPDIEEFAKADKEKKLLNMMRGFRA
                     IGTALLLPPATPKDRIEILKEAVRKTHTDPAFINEYRKLTGGDEPTPLMPDEQAKVVK
                     EIPRDAETIELFKKFAGTGPLPAR"
     gene            complement(1738..1998)
                     /locus_tag="VF182_12015"
     CDS             complement(1738..1998)
                     /locus_tag="VF182_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437851.1"
                     /translation="MILLEKYPLITGISLADEARYGSNSMKQSTSSYTDQNWSWPQST
                     WFQKRLNAIKAQEEAARQKHPVPQKPTGADTKSNKIEPSEDF"
     gene            2307..2945
                     /locus_tag="VF182_12020"
     CDS             2307..2945
                     /locus_tag="VF182_12020"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX6437852.1"
                     /translation="MNTNKYRFILLGLMVAGLGILIGTILWIKLAPQPRFVGSARSSL
                     EEMSHYGSVPDFSLIERSGRTIGLEQLRGTIWVADFIYTDCQDTCPLQSAEMARLQDE
                     LPAQKAIKLVSFSVNPDKDTRLVLSQYADRFKASSDRWLFLTGGHEEIKNLVQNGFRL
                     SVATASTDPGVILHSPRLVLIDRQAQIRGYYDSRDNQALQRLRRDVAILAKG"
     gene            2958..3839
                     /locus_tag="VF182_12025"
     CDS             2958..3839
                     /locus_tag="VF182_12025"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX6437853.1"
                     /translation="MAKTSKNRPTASIQAAAPSTAKQHPNWPILALAFAGMILSGYLT
                     YSAWQGQSVAGCTVGSACDIVLNSRWSKLLGVPTSFWGFLTYALLAAIAWNKTTPSQW
                     KMAWTVTVIGLLYSLYLTGVSLIILESACPYCLTSLALMGAIFAVLLMRRPAKMPGFS
                     WRPWLGKTAGAAVLLIIALHLHYSGYIGKTAGPEDPWVRGLVEHLVKTDAKFYGASWC
                     PHCADQKELFGPSRSRLPYIECSPGGPRAPQAQACNDAGIKSYPTWIINGQRYSGIQS
                     LDSLAQVSNYKTEGAKK"
     gene            3836..4162
                     /locus_tag="VF182_12030"
     CDS             3836..4162
                     /locus_tag="VF182_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6437854.1"
                     /translation="MKTFISRTSLLFAFWVVSSLAYYHTASADPSKYPQFAQQSLPAN
                     VPLVLTSIDELVADVKAGKKPLIIDVRTMAEYQEVHILGALSAPLAEFKEYMKSIPRD
                     RPVVLY"
     gene            4311..5012
                     /gene="queC"
                     /locus_tag="VF182_12035"
     CDS             4311..5012
                     /gene="queC"
                     /locus_tag="VF182_12035"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX6437855.1"
                     /translation="MARKKAVILLSGGIDSATAAAIARHNRFDLYALTFRYGQRHERE
                     IKSANKVAVWLGVEEHVIIDADLRAIGGSALTDRIEVPKGRSTSEIEHGIPITYVPAR
                     NTIFLAYGLALTEKIAASDIFFGANQLDYSGYPDCRTEYVRAFEKMANLATKAAVQGR
                     TRLKIHTPLIQLTKAEIIKQGSELGLDYGLTWSCYDPTDEGKACALCDSCQLRLKGFK
                     EAGLSDPIPYAAIAS"
     gene            5144..5914
                     /locus_tag="VF182_12040"
     CDS             5144..5914
                     /locus_tag="VF182_12040"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX6437856.1"
                     /translation="MEFADYRRYSPGDDLRYLDWGIYARTDRLYIKVFREEVDLFAYM
                     FIDASASMAFPSQEEKFLPAAHIGLALSYVILANHDHVKLHLLHGDSMSPASPFYRGR
                     RRMADCVRFATSLEPRGRLDLAGSLSEHLKKLRRPGKAILISDFMMPAQSYQRGLNLL
                     RALNLDIAVIQVLSRPEIDPPFTTGGLNLVDSETQGEIRLQWSARARQEYQAKLAHHN
                     SELRSFCHQSGIHYSLYVNDRDLGDFVFATLPAIGLFK"
     gene            5924..7744
                     /locus_tag="VF182_12045"
     CDS             5924..7744
                     /locus_tag="VF182_12045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6437857.1"
                     /translation="MEFLNPAALYGLFALPLLLIPYLVRRKPRRVIFSSLLLFIEHGS
                     EATAKPWGRLRLPLVFFLQLLLLVLLILALAQPVFSVRLSNIAIVMDNSASMQAREDG
                     TTRVALARDQARRVLGDLGANGKVDIFTIVPQLEKANKAPLSPSDAIALLAGLEAYDL
                     ADAPADYGDLLRQLAEDHKYERVYFVTDRPVRGQSGAVRVLTVGKPRDNLALSAFTVS
                     PSSLANRRLEAVVEVANYSARDQRVRVAVLGDGAETALANRDLRVPAGQTASASFQGL
                     SEKRYYIAEIDGNDALSLDNRQFAIAANARSLKILAISPRPQALNSLRAIPGVTLTIV
                     APEDYQQTDRSSFGLEIFHYAALASLPLNPSLLILPPDSNPIVKLGNPITGPVASGWR
                     ESHPLSRYLNFALFRPTYARPIIPLTAGEAIIETVQGPLAFTVERQGNRHLVLGFDPF
                     PYLGRENLPMSIFTLNLLEWFTSVSGGKGRATGEPVALGAAEQESSILTTPQGQKHVL
                     KPGSSSFANTFHQGIYQLARGAERQILAINYHSASESDLRDSKRIELKPGASGSNSGS
                     TLFSFWPYLLMASLLLLIIEWFFVPPADRRRLPVTAPRPT"
     gene            7753..10302
                     /locus_tag="VF182_12050"
     CDS             7753..10302
                     /locus_tag="VF182_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6437858.1"
                     /translation="MADVEFAKPLVFWLLIGLPVLWFWFADRRWFVLIWRTLILAILI
                     VALADPQFVTKQTRRESEDRIFAFDVSKSISPNMRQWMDSSTQDALAPDDTDHTLVFA
                     GEAKDTSKWRDWIKSGPAVQDGIRPENTNLQKLFENLLSLPSAPRSLYLFTDGWENQG
                     NLESMLPAIAASGLKVYPIVPTQQLGLANVTVSKLVAPTQGDAGEAVNIKVVLKNQNS
                     REVDGTLTLTRNGVTFKTDPVKLKPGSQILSYQSTLADGPLNSYRASFTARQDGSDQF
                     EPDNHALAWTSVRSKGKILIVSGRTEGGRYIEEILRRQGFEVTSRSAAAAPTPNGFNI
                     VIFNNVEREKLTAAYLSAIERYVSGGNGFIMLGSEWSFGPGGYRRTPIETVLPVEFKD
                     PPKQEEKNRAIILVIDKSGSMREDNRILYAQEAAKAVARQLKDTDLLGVIGFDVEPFV
                     VVPLASVGSLRGSFNTQIERLKPAGRTVMLPAIIEAKRQLERQNAGRKHVIVLSDGET
                     GGSGGDFIDLVNVMKAEQQITVSAIAIGAEANIPLLKRITQYGGGFFHHIYDPTTLPQ
                     IVLQRVQEKQKDEPPPPERDLIPVQERGSELLAGLSGKAYPPLRGFIETELKRGAQLD
                     LSIAKEGRKAPLLASWRYGRGKAVALTTDLEGRWSRAWIQWGGLPEFWEKVLDWIRPT
                     ADTPIPLHETRISLLGSQPILDLYLYEETPSDSQFRFTASGNGTKSEGILKRIATGHL
                     QAALPTLPVGEYRIDLVEERKGRQIAYPPVGFTATYDPKRELPRPDVNLALLRRLAEA
                     SSGEINPISVEKMERQHTTAVHQPVRAPLIVLAFGLFIFEIAVRRLLLGES"
     gene            10381..11766
                     /locus_tag="VF182_12055"
     CDS             10381..11766
                     /locus_tag="VF182_12055"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX6437859.1"
                     /translation="MQLRFPYGDPESRCGQALCFKGFRAGYSLLIWFAVLIATGATKP
                     ADAETIKQKSVRKVGKGKVSAPTPPIRRLTNNTLLPPRESTFPDLWQRQEHIIHRIRS
                     GDTLPGILDLFSLTPGEKELWYRSVRQHFRSGKLAAGRQVHLYFANPNIDDNRGGESL
                     MAVEVDFDDAMDLTWEKNGRSVFFARREKPVDVELRTATATIDDSLFTSGMKAGIPSS
                     LLSQLVDIFTWDVDVEKEIHQGASFKILYEQRSTRGQSDKAERRILAAELTNAGQKMT
                     AIYFEKQKGIGSYYNAEGRSLARSFLRFPVEFTNITSKFSDSRFHPVLKTSLPHTGVD
                     FAAERGTPVRAVGDGVINQAGWSGSYGKLVEIQHDATFTTRYAHLDGYGRGVQRGTVV
                     KKGQIIGFVGSTGRTTGPHLHFELYKDQQYINPFSLDFPAEDTIEPALLTLFEDQKNT
                     FLMELTSLPQS"
     gene            12060..13301
                     /locus_tag="VF182_12060"
     CDS             12060..13301
                     /locus_tag="VF182_12060"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX6437860.1"
                     /translation="MMQKAVILSTGDELTTGRVIDTNSAAIAKRLSTIGMEVQAVLKV
                     GDNKDQLLWALRQARALGDIAIGSGGLGPTSDDLTTEVVAEFLGCDLRQDEQIARELQ
                     QRFKERRLSWSANSLKQALFPGGATIISNPVGTAPGFYVPIGQDKTLVWLSGVPQEMV
                     AMLDQTVLPWIERQRDKPLQAFSCTFKIYGVTEGKLDELLRPVNLGNYGKLSFRAHYP
                     DLSLRLTVRTQRADGTVLFETLKDAIRAILSKYIYAEGDTTLEEVIGRLLTDKQQTLA
                     LAESCTGGYISHRITRIAGSSAYYYGGAVTYSNEAKMRFLGVRPETLQEHGAVSRETS
                     LEMSAGIKHRTGADVGLSVTGVAGPGGGSATKPVGTVWVSIAKTGSHEAKLFRFAGDR
                     EYIILGTSQAALNWLRISLLQ"
     gene            13317..13880
                     /gene="thpR"
                     /locus_tag="VF182_12065"
     CDS             13317..13880
                     /gene="thpR"
                     /locus_tag="VF182_12065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX6437861.1"
                     /translation="MIRAFIGVQIAPEVRARISAAITQLTPAISGIRWVREENFHFTL
                     KFLGLIEEPQIEPISNAFAQAILPFRRFTINAKGLGVFPDIKKARVLWVGLEGNDLTA
                     LAKSVESVLEPFGFPRENRIFRPHLTVGRWRHPRGSQQELGGKLKRWKNFEFGAFAVD
                     QVVLFQSVLKSDAAVYRPLKTVSLAGD"
     gene            13920..14972
                     /gene="recA"
                     /locus_tag="VF182_12070"
     CDS             13920..14972
                     /gene="recA"
                     /locus_tag="VF182_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX6437862.1"
                     /translation="MDINREKALDLAVSQIEKQFGKGAIMKLGEGGVTKDVPVISTGS
                     LGLDIALGIGGVPRGRVIEIYGPESSGKTTLALQIVAEAQKQGGMGAYVDAEHALDLG
                     YAKKLGVKTDDLLVSQPDHGEQALEIAETLVRSGAIDVLVIDSVAALVPKAEIEGEMG
                     DSHMGLQARLMSQALRKLTGTIARSHTVVIFINQIRMKIGVMFGNPETTTGGNALKFY
                     ASVRMDIRRIGALKDGDNIVGGRTRVKVVKNKMAPPFKEAEFDVLYGTGISRDGEIVD
                     LGSEMGIVEKSGAWYSFAGDRIGQGREAAKNFLKEHPEIAQQIMSRVMERVGLKREEA
                     PVTTGNVDRKEKAKVK"
     gene            15492..>16912
                     /gene="alaS"
                     /locus_tag="VF182_12075"
     CDS             15492..>16912
                     /gene="alaS"
                     /locus_tag="VF182_12075"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX6437863.1"
                     /translation="MMTGNDIRRSFLDYFTKQSHTVVKSSSLVPEKDPTLLFTNAGMV
                     QFKNVFLGQERLSYSRATSSQKCLRISGKHNDLEAVGRDTYHHTFFEMLGNWSFGDYY
                     KAEAIEWAWELLTREWSLPKEKLWATVYLDDDEAERLWPRISGLPAERIRRFGEKENF
                     WEMGETGPCGPCSEIHLDRGPEACDRKGLPGHVCQVNGDCARYIELWNLVFIQYNRKE
                     NRELEELPSKHVDTGMGLERITAVMQNVLSNYDIDYMRELTAAVENLAGKRYGADATA
                     DISFRVIDDHARAVSFLIADGVMPSNDGRGYVLRRLLRRAARHGRLIGLKEPFLYQIA
                     STVGKVMGEAYPELQTEAPRIREIIRSEEERFSETLEKGLVLLEDAAAKLKQENKKML
                     PGEVAFRLYDTYGFPLDLTEDILRAENISVDQHGFENLMDTQRSRGREARAIAMDVFD
                     QVAVADTVIATKFIGYDQLEAEST"
BASE COUNT         3970 a         4476 c         4574 g         3892 t
ORIGIN      
        1 ggccagtcct cgcgggcatg atctacgaca agactcaaac gtatgggatg gtcttatgga
       61 tcttcttgac tctgttgatc attgcgacac tgttggtgtt gtcgttaatt cgagcatgga
      121 agattcgcat gatcgggacc ggctctccgg tctcgtcaag tgcaacgact gcttagcctc
      181 gcgctatcaa gatttattcg ggtgggtttc gttcaaagtg aacgttaaag cacgtcatgg
      241 aggcttactt cggcacgctt gtaatcgccg gcggcacagc tcatttgcct tgacgtaaat
      301 tgtcaatgcg ctatctctat cgaatcgtgc cataagattt tatcggaact ggtttgaccg
      361 aaggagtcaa tatgtcccgc tttggatcca agtacactct cgctctcgcc ttcttttttc
      421 ttgtctcggc tgtgctgacc acgattttta tcaatccagt tttttcacag gcgcccttct
      481 atcagggaaa aacgatcacg cttatcatcg gcgccggtcc aggcggaatg ggtgacttgc
      541 gcgcccgcgc cctggcgtca gttctggtca aacacattcc gggaaatccc acaatcactt
      601 ttcaatatat ggccggggct ggcggacgta aggcggccaa tcatctttac aacaccgctc
      661 gggccgatgg tcttacgctg tttcggatct ccagcagcat cataccttac gcggttttag
      721 gggaatctgg tgttcagtac gatatagaca agctcgttta cctgggaacg acggaacata
      781 tcctttatta tttgtttaat acacgcaagc aactgggtct ggatactttg gccaaactcc
      841 gcgctgcgtc aggagtccgc atcggcagcg ctcctgtggg ccatactggg tatatccaga
      901 gccgcgtgac tgcctacctg ctcgacatga aggagcctcg tttcattccc ggatatgaaa
      961 atgaagatct cgacgtcgct ttcgcgcgtg gggaggtgga cgcgcaggtc gcttcaacaa
     1021 gtacggtgat tgagcacgat ctgttaaaca aaaaattagt cgactttcac gccgccatcg
     1081 aagtgccgaa aggacgaaaa gactctcgtt tcgcaaaatt aaatctgccg gacatcgaag
     1141 agttcgccaa ggcggataaa gagaaaaagc ttctgaacat gatgcgtggg ttccgagcca
     1201 tcggcacggc tctgctcttg cccccggcaa ctcccaaaga tcgtatcgag attctcaaag
     1261 aggccgtgcg aaaaacccat acagatccgg cattcatcaa cgagtaccgg aaactcaccg
     1321 gcggcgatga accgacgccg ctgatgcctg atgagcaagc gaaagttgtc aaggaaattc
     1381 ctcgcgatgc tgaaacgata gagctgttca aaaagtttgc cggaaccgga ccactcccag
     1441 cgcgctaagt taccgactgt ccatagtcgt gcttaccgtg tgcgggattt aacatgcaag
     1501 ctaaaatggc tgcatcggta gatcctaagc cgcgcttcaa tgttccctcc agctctttcc
     1561 gcggcggccg ctagagaagc tacgatgttg agagttgata tctctcttgt gcgtctgcgc
     1621 tttggaaaca gctggccgat aatcgcgaat atccgccgca caccggcgtc aagaaagtga
     1681 taggaaaccc ttggatggag caagctgatt ggaatcggga gagtagagta gcgggtctta
     1741 gaagtcttcg gaaggctcaa tcttatttga ttttgtatcg gcgccggtcg gcttttgcgg
     1801 aaccggatgc ttttgccgcg ccgcctcttc ctgcgctttg attgcgttga gacgtttctg
     1861 gaaccaagtc gactgtggcc agctccagtt ctgatccgtg tagcttgaag tggactgttt
     1921 cattgagtta ctcccgtatc tggcctcatc agcaagcgat atgccagtaa tcaacgggta
     1981 tttctcgagc agaatcatga cgaagaccgc cactgtcatg acaggactca gccattttcg
     2041 ctcaaattcg aacagtcaat tttgccgaag aggtaacgta aatagcgcct ccgtgcagag
     2101 atctctccga tctggtccga tccctcaggg ctgatgacgg tcatcaataa gctgagtaaa
     2161 tgcaattcgt cgcgctgcgg cgcaattgta gaatcggcgg aatcggcgat acaacaacgc
     2221 gtcttcaccg tcgtcgctgg caattgagcc ggcctctaca caaaccgcaa attaatcgat
     2281 aaacttcctc ccggtaagat ttactcatga atactaataa ataccggttt attctgttgg
     2341 gattaatggt tgccggatta ggcatcctca tcggtacgat actctggatc aagttggctc
     2401 cgcagccccg tttcgtcggc agcgcgcgga gttctttgga agaaatgagc cactacggct
     2461 ctgtgcccga tttctcatta atagaaagaa gcggcaggac catcgggctc gaacaactgc
     2521 gcggcacaat ctgggttgcc gactttattt ataccgattg ccaggatacc tgcccgctac
     2581 agagcgcaga aatggctagg ctccaagatg aactgccagc gcagaaagcc atcaagctag
     2641 tgtccttttc tgtcaacccc gacaaagata cgcgcttggt tctctcgcag tatgcagatc
     2701 gatttaaagc gagcagcgat cgttggctgt ttttaaccgg cgggcacgaa gagatcaaaa
     2761 acctagtgca gaacggcttc cgcttgagcg ttgctaccgc ctctactgat cctggagtga
     2821 ttcttcatag tccgcgattg gtattgatcg accggcaagc acaaatccgc ggctattacg
     2881 atagccgcga caaccaggcg cttcagcgcc ttcgcaggga cgtcgccatc ttagcaaagg
     2941 gttgaggatt tagtcacatg gcaaagacaa gtaagaacag gcccaccgcc agtatccagg
     3001 cggcagcacc gagcacggcc aaacagcatc cgaactggcc aatactcgct ctggcgttcg
     3061 ccggcatgat tctatccggc tatctgacct attccgcttg gcagggccaa tcggtagccg
     3121 gatgcacagt cggaagcgcg tgcgatatcg tactcaacag tcgatggtcg aaactgttgg
     3181 gcgtgccgac ttccttctgg ggttttttaa cctacgccct tctagctgcc attgcctgga
     3241 acaaaaccac gccgagtcag tggaagatgg cctggaccgt cacggttatc ggattgcttt
     3301 acagcctcta tcttaccggc gtttcattga tcattttaga gtccgcttgt ccctactgtt
     3361 taacgtcact agcattgatg ggagccattt ttgccgtgct tctcatgcgc aggcctgcta
     3421 aaatgccagg cttctcttgg cgaccatggc tcgggaaaac tgccggagcg gcggtcctac
     3481 tcatcatcgc gctccacctt cattattcag gttacatcgg aaaaaccgcc ggcccggagg
     3541 atccctgggt gcgcggcctg gttgaacatc tggtcaaaac cgatgcgaaa ttttacggcg
     3601 cgtcgtggtg cccccattgc gccgaccaaa aggaactttt tggcccgtct cgctcccggc
     3661 taccgtacat cgaatgcagc ccgggtggcc cgcgggcgcc gcaagcgcaa gcatgcaatg
     3721 atgccggcat caagagctac ccgacctgga ttatcaatgg gcaacgctat tccggcatcc
     3781 aatcgttgga cagcttggct caggtcagta actacaagac cgaaggagca aagaagtgaa
     3841 gacatttatt tcccggacga gtctcttgtt tgcattttgg gtggtgtcct cactggcgta
     3901 ctatcataca gcaagcgccg acccatctaa gtaccctcaa tttgcccaac aatcgctgcc
     3961 ggctaacgtg ccgctagttc ttacatcgat tgatgagttg gtggctgacg taaaagcagg
     4021 caagaagcct ctgattatcg atgttcgaac catggcggaa taccaggaag ttcatatcct
     4081 cggagccctt tcagcaccac tggccgagtt taaagaatac atgaagagta tcccgaggga
     4141 tcggccggtt gttctttact gagcctgccc gcatcacctg gccagtttgg cttatggcat
     4201 tctctatgaa aatggctacc gcaatatgaa ggttctgaac gaaggcttgg tcggctggta
     4261 cgaaaaacgt tacccaatgg aaggaaccaa ggttccttga atccggggtc atggcgcgca
     4321 aaaaagcagt catcttactt agcggcggca tcgattccgc caccgcggca gcgattgcgc
     4381 gccacaatcg cttcgatctt tacgccctca ccttccgcta tggccaacgg catgaacgtg
     4441 agattaaatc cgctaacaag gtcgcagtct ggttgggagt tgaggagcac gtcatcattg
     4501 atgccgacct ccgtgcaatt ggcggttcgg ctctgacgga ccgtatagag gttcccaaag
     4561 gccgctccac atccgaaatt gagcacggca taccgataac ttacgtgccg gcacgcaata
     4621 ccatctttct cgcatatggc ttggcattga ccgaaaagat tgccgcatcg gacatttttt
     4681 ttggcgctaa ccagcttgat tatagcggct atccggattg ccgcaccgaa tacgttcgcg
     4741 cctttgaaaa gatggccaat ctggcgacca aagccgccgt ccaaggccga acgcgcttga
     4801 agattcacac cccgttgatt caactgacaa aggcagaaat catcaagcaa ggcagcgagc
     4861 tgggactcga ctacggtctt acctggagtt gctacgatcc aaccgacgaa ggcaaagctt
     4921 gcgctctgtg cgacagctgt caactaaggc taaaaggttt caaggaggca ggacttagcg
     4981 atccaattcc atacgcggcc atcgcgagtt agatctttca atgctccctg aaccctttac
     5041 gccggaattc ctcacacagt tggaaacgct tcgtttacgc accaggaaag aatttctcgg
     5101 cagccatacc ggcagctatt catcaccccg gcgcggcaca agcttggagt ttgccgatta
     5161 ccggcgttat tcccccggag atgatctgcg ctatctggat tggggcatct atgcgcgcac
     5221 cgatcggctt tatataaagg tgttccggga agaggttgat ctcttcgcct acatgttcat
     5281 cgatgccagc gcatcgatgg cattcccatc gcaggaagaa aaattcttac ccgccgcgca
     5341 tatcggacta gcgctttcct atgtaatttt ggctaaccat gaccatgtca agctccattt
     5401 gttacacggc gactcgatga gcccggcctc ccccttctat cgcggacgtc gccgcatggc
     5461 tgattgcgtg cgctttgcaa cgtcattaga gccgcgcggt cgcctcgatt tagcgggatc
     5521 gctgagtgag catcttaaga aactgcggcg gccaggcaag gcaatcttga tctccgattt
     5581 tatgatgcca gcacaatcct accagcgcgg actgaacttg ttgcgagccc ttaatctgga
     5641 tatcgcggtc atccaggtac tgagccggcc tgaaatagat ccaccattta ctacgggtgg
     5701 cttaaacttg gtggacagtg aaacgcaagg tgagattcgg ctgcaatgga gtgcccgcgc
     5761 gcggcaagag taccaggcga agctggcgca tcataactca gaactacgca gtttctgtca
     5821 tcaaagcggc attcattact ccctatatgt taatgaccgt gatctggggg atttcgtttt
     5881 cgccacctta ccggcgatcg gtttgttcaa ataagcttgg ctcatggagt ttctcaatcc
     5941 cgcggctctc tacggactct ttgcgctgcc cttgctcctg attccctatc tcgtccggcg
     6001 taaaccacgc cgtgtgatct tttcaagcct gctgttgttc atagagcatg gcagtgaggc
     6061 aactgcaaag ccctggggcc gcctgcgcct tccgctggtt ttttttcttc aattgctcct
     6121 actcgtgtta ttgattttgg cgcttgccca accggtattc tcggttcggc tatccaatat
     6181 cgccatcgtc atggacaatt cggctagcat gcaggcgcgc gaggatggca caactcgtgt
     6241 ggcactcgcc cgagatcagg ccaggagagt gttgggtgac ctcggtgcaa acggcaaagt
     6301 agatatcttc accatcgtgc cgcagcttga aaaggctaac aaagcgcctc tttctccgtc
     6361 tgatgccata gcactgctgg ccggtctaga agcctatgac ctcgctgatg cgccggctga
     6421 ttacggcgat ctattaaggc agctcgccga ggatcacaaa tatgagcgtg tctacttcgt
     6481 taccgatcgg cccgtacgag gccaatcggg tgccgttcgg gttcttaccg tgggaaaacc
     6541 gcgagacaat ctggcgctaa gcgcatttac cgtctcgccg tcttcgctgg ctaacaggcg
     6601 tttagaggcg gtggtcgaag ttgccaatta ttccgcccgc gatcaacgcg tacgagtcgc
     6661 agttctgggt gatggcgctg aaactgcgct agccaatcgc gatcttcgcg tcccggcagg
     6721 acaaaccgct tccgcatcgt ttcagggcct ctccgagaag cgatactata ttgctgaaat
     6781 cgacggcaac gacgctttga gcctcgataa tcggcaattt gccattgccg caaatgcgcg
     6841 cagcttaaaa atcctggcga tttcccctcg acctcaagcg ctgaacagtt tgcgcgccat
     6901 ccccggggtt accctgacca tcgttgcgcc ggaagattac cagcagaccg atcgttccag
     6961 ttttggcttg gagatttttc actatgcagc gctggcctcg ttgccgctca atccatcgct
     7021 gcttatcttg cctcccgaca gcaatccgat tgtcaaactg ggcaatccga ttaccggacc
     7081 ggtggcctcg ggttggcgcg aatctcatcc actgagccga tacctcaact ttgccctatt
     7141 ccgcccaacc tatgcccggc ctataattcc gctaactgcc ggtgaagcaa tcatcgaaac
     7201 ggttcaagga ccacttgcct tcacagtcga gcgccagggc aacaggcacc tggtactcgg
     7261 gtttgaccct tttccttatt tgggccgaga gaatttgccg atgtcgattt ttacgctcaa
     7321 tcttcttgag tggtttacat cggtcagcgg cggcaaaggg cgagccacgg gcgagccggt
     7381 tgccttggga gctgccgaac aagagtcgtc aatcctcacc accccgcaag ggcaaaagca
     7441 tgtgcttaaa ccgggctcta gtagctttgc taataccttt caccagggca tttaccaatt
     7501 agctcgaggt gccgagcggc agatattggc gatcaactat cactcggcca gtgagtcgga
     7561 tctgcgggac agcaagcgca tcgaattaaa gccgggcgcg agcggcagca acagcggttc
     7621 gacacttttc tctttttggc cctatctgct aatggcgtca ttactgcttt tgattatcga
     7681 gtggttcttt gtcccgccag ccgaccgcag acgattgccg gtgacagcgc ctcggcccac
     7741 atgaacactc tgttggcaga tgtagagttt gctaagccat tggttttctg gctgctgatt
     7801 ggactgccgg tactttggtt ttggttcgcc gaccggagat ggttcgtcct catttggcgc
     7861 acgctgattt tggctatctt aatcgtcgct ttggccgatc cgcaattcgt caccaaacag
     7921 acccgtcgtg aaagcgaaga ccgtattttc gcgttcgacg tttcgaaaag tatttccccg
     7981 aatatgcgcc aatggatgga cagctcgacg caagatgcgc ttgctcctga tgacaccgat
     8041 cacacgttgg ttttcgctgg cgaggcaaag gacacttcaa agtggcgcga ttggataaaa
     8101 agcggccctg cggtgcagga cggcattcgg ccggagaata ctaatcttca gaagcttttc
     8161 gaaaacctgc tctcgctccc gtcggcgccc cgcagcctct acttatttac cgatgggtgg
     8221 gagaaccagg gcaatctaga atcgatgttg cccgccattg ccgcctccgg tttgaaagtt
     8281 tatccgattg tgccgacgca gcagttgggc cttgccaacg taactgtttc caaactcgtg
     8341 gcgccgactc agggagacgc cggcgaagcg gtaaacatca aagtagtgct gaagaaccag
     8401 aatagtcgcg aggtcgatgg aactttgact ctgacccgca acggcgtaac cttcaaaacc
     8461 gatcctgtga agctaaaacc cggcagtcag attttaagct atcaaagcac gcttgcggac
     8521 ggaccgctaa attcgtatcg cgccagcttc acagcccgtc aggacgggtc cgatcaattt
     8581 gaaccggata accacgcgct cgcctggacc agcgtgcgta gcaagggaaa aatcctcatc
     8641 gtcagcggtc gaactgaggg cggaaggtac attgaagaga tcctgcgccg ccagggtttc
     8701 gaagtgacct cgcgcagcgc cgctgccgca ccgacgccaa acggcttcaa tatagtgatt
     8761 tttaacaatg tcgaacgcga aaaactcacc gcagcatatc tcagcgcaat cgaaaggtac
     8821 gttagcggcg gcaatggctt cataatgctg ggcagtgagt ggagtttcgg accgggggga
     8881 tatcgccgca ccccaatcga aacggttttg ccggttgagt ttaaagatcc gcccaagcag
     8941 gaagagaaaa atcgagccat tatactggtc atcgataaat ccggtagtat gcgggaagat
     9001 aaccggatct tgtacgctca agaggctgcc aaggctgtcg cgcggcagtt gaaggacacc
     9061 gatctgctcg gggtcattgg ctttgacgtt gagccattcg tggtggtgcc gctcgccagc
     9121 gttggcagcc taagaggatc attcaataca cagatcgagc ggctgaaacc tgcgggtcga
     9181 accgtgatgc ttccggctat catagaagct aaacgacagc ttgaaagaca aaatgccggg
     9241 cgcaaacatg tcatcgtgct cagcgatgga gaaaccggcg gaagcggcgg cgatttcatc
     9301 gatctggtca acgtcatgaa ggccgaacaa caaattacgg tgtcggccat tgctattggc
     9361 gcagaagcaa acataccgct cctgaagaga atcacccagt acggcggcgg cttttttcac
     9421 cacatctacg atccgacgac gctgccgcag attgtgctgc agcgggttca ggagaagcaa
     9481 aaagacgagc cgccaccacc ggaacgcgac ttgatacctg tccaagaaag aggctcggaa
     9541 ctgctggcag gcttatcggg caaagcatat ccgccgttgc gcggttttat cgaaaccgag
     9601 ctcaagcgcg gcgcgcagtt ggatctctca atcgccaaag agggacggaa agctccactg
     9661 cttgcatcct ggcgctacgg ccgaggcaaa gcagtcgctt taaccacaga cctggaaggg
     9721 cgatggagtc gagcttggat ccagtgggga ggcctgccgg agttttggga aaaggttctg
     9781 gactggattc gccccaccgc tgataccccc ataccacttc acgaaacaag gattagcttg
     9841 ctcggcagcc agccaatcct ggatctttat ctatatgagg agacaccttc agatagtcaa
     9901 tttcgcttca cagcaagcgg aaacggcact aaaagcgaag gtatactcaa aagaatcgct
     9961 accggtcatt tacaggctgc cctgccgacc ttacctgtcg gtgaatatcg gatcgatctt
    10021 gtggaagagc gcaagggtcg ccagatcgcc taccctcctg tgggatttac cgcgacgtac
    10081 gaccccaaac gtgagttgcc gcgccccgac gtaaacttag cactgcttag gagactagct
    10141 gaggcgagca gcggggaaat aaaccctatt tccgtagaaa aaatggaaag acaacatact
    10201 accgccgttc atcaacctgt tcgggcgccg ttgatcgtcc ttgcatttgg gctttttatt
    10261 tttgaaatcg ccgtaagaag gttgcttttg ggcgagtcct gatccgcttc catgggatcc
    10321 cgctacccgg attcggggtt gacacaccca gctaaacggt ttatttagtg taaagtttgt
    10381 atgcaacttc gattcccata tggggaccct gaaagccgct gcggccaagc gctgtgcttc
    10441 aaagggtttc gcgctggtta tagtctactg atatggtttg ccgtgcttat agcaactgga
    10501 gcgacgaagc cggcagacgc cgagaccatt aaacaaaaat cggtgcgcaa ggtcggcaag
    10561 ggtaaagtat cggcccctac tccgccgatc cggcggctga cgaataacac gttattgccg
    10621 ccgcgggagt caacgttccc cgatctttgg cagaggcagg aacacattat tcaccgtatc
    10681 cgttctggag ataccctacc cggaatcctg gacctattca gcttgacccc cggcgagaag
    10741 gagctctggt accgctcggt ccgccaacat ttccgatccg gaaagttggc tgccggaaga
    10801 caagttcatc tctatttcgc caacccgaac atcgatgata acaggggtgg cgagtcgttg
    10861 atggcggtgg aggtcgattt cgatgacgcc atggatctga cgtgggagaa gaacggccgg
    10921 agtgtttttt ttgcacgacg ggaaaagccg gtcgatgttg agttgagaac cgccaccgca
    10981 actatcgacg actcgctatt cacatcgggt atgaaggccg gtatcccaag cagcctactt
    11041 tcacagttgg tcgatatttt tacctgggat gttgatgttg agaaagagat ccatcaaggc
    11101 gctagcttca agatattata cgaacaacgc agcacacggg gccaatcgga caaggccgaa
    11161 cggcggattc tggccgcaga attaaccaat gctgggcaaa aaatgactgc catctatttt
    11221 gagaagcaga aaggcatcgg ctcgtattac aacgccgagg gcagaagttt agctaggtcc
    11281 tttcttcgat tcccggtgga atttaccaat atcacctcaa agttcagcga ctcacggttc
    11341 catccggttc taaagaccag cctgccgcac accggtgtcg attttgccgc ggaacggggc
    11401 acgccggtcc gagctgtggg cgacggagtt atcaatcaag ccggttggag tggcagttat
    11461 ggcaaattag tcgaaatcca gcatgatgcc acctttacga ctcgctacgc ccatctcgac
    11521 ggttatgggc gtggggtcca aagaggtacc gtcgttaaaa agggacaaat cattggattt
    11581 gtcggttcca ccggcagaac caccgggccc cacctccatt tcgagctcta taaggatcaa
    11641 caatatataa acccattcag cctcgatttt cctgccgaag ataccataga gcccgcattg
    11701 ttaactctgt ttgaagacca gaaaaatacc ttcctaatgg aattgacctc cttgcctcaa
    11761 tcctaagttg gttgcctgcg gctctcgccg tgatcctgta gcagtcttaa aatcgtctag
    11821 aacctcagtt tctacctgtg cgcagttcag gtcttcgcgc atcgctgtca tggttctgaa
    11881 gtaaacgcaa ctgagatttg ccgataacga ggccggaagg ctatcgtccg gttgtaggca
    11941 accccctcgg cagctaatct ctattgatat cgatgatttc agcagggctt ctggacgatg
    12001 cgaaaatcgg gttgtacact ctgatcgtcc cgcagctacc ttagcgatta ggcactgaca
    12061 tgatgcaaaa ggccgtgatc ttgagcaccg gcgacgaact caccaccgga cgggtgatcg
    12121 acacgaactc ggcagctatc gcgaaacgcc tttccaccat cgggatggag gtgcaagcgg
    12181 ttctgaaagt cggcgacaac aaagatcaac tcctgtgggc gcttcgccaa gcccgggcgc
    12241 tgggggacat tgcgatcggt agcggcggcc ttggaccaac cagcgatgat ctcacaaccg
    12301 aagtcgtcgc ggagtttctc ggatgtgacc ttcgacagga cgaacaaatc gcgagagaac
    12361 ttcagcagcg cttcaaagag cgacgtctca gttggtcggc caattccttg aagcaggcgc
    12421 tgtttccagg gggagcgacg atcatttcta atcccgtggg aacggcaccc ggtttttacg
    12481 tgccgattgg gcaggataag acgctcgtgt ggctttccgg ggtgccgcaa gaaatggtcg
    12541 ctatgctcga tcagacggtt ttaccgtgga tcgagcgcca gcgggacaaa cctctacagg
    12601 ctttttcctg cacgttcaag atttacggag tcactgaggg caagcttgat gaactgctac
    12661 gacctgtgaa tttggggaac tatgggaagt tatcctttcg cgcgcattat cccgatcttt
    12721 ctttacggct aaccgttcga actcagcgcg cggatggaac agttcttttt gagactctca
    12781 aagacgcaat cagggcaatc ctgagcaagt acatttatgc cgaaggcgat accactctgg
    12841 aagaggttat cggccgatta cttacggaca agcagcaaac tcttgccctg gcggagtctt
    12901 gcaccggcgg ttacatcagc caccggataa ctcgcattgc cggtagctcc gcttattact
    12961 atggcggtgc ggtaacttat tccaatgagg cgaagatgcg gtttctcggc gtccgcccgg
    13021 aaactttaca agaacacggt gccgtcagcc gtgaaacgtc cctagaaatg tcggcgggta
    13081 tcaagcaccg caccggtgca gatgttgggc tgagcgtcac cggtgtcgcg ggtcctggag
    13141 gaggcagcgc gacaaagccc gtcggcacag tttgggtcag tatcgctaaa actggaagcc
    13201 atgaagcgaa gctttttagg ttcgctggcg atcgcgagta cattattctc ggcacgtcgc
    13261 aggctgcgct gaattggctc cgtatcagcc ttctccaatg aggttaagcg ttagccgtga
    13321 ttcgcgcgtt tatcggagtg cagattgctc ccgaggtccg cgcccgaatt tccgcagcga
    13381 taacgcagtt gaccccggca atttccggga tccgctgggt ccgagaagag aacttccatt
    13441 ttacgctgaa gtttctcggt cttatagaag agccgcaaat cgagcccatc agtaatgctt
    13501 ttgctcaagc gattctgccc tttcggcgtt ttactattaa tgctaaaggt ttgggagtat
    13561 tccccgatat taaaaaggct cgcgtgttat gggtcggtct ggaagggaat gacctgacgg
    13621 ccctggcaaa gagtgtcgaa tctgtgttag aacccttcgg ctttccccgt gaaaacagaa
    13681 tctttcggcc ccacttaacc gtaggccgat ggcgccatcc gaggggatca cagcaagaac
    13741 tcgggggtaa gctcaagcgc tggaagaatt ttgagttcgg cgcattcgct gtcgatcagg
    13801 tggtgctttt tcaaagcgtg cttaaatcgg acgccgcggt gtatcgaccg cttaaaacgg
    13861 tctcgctcgc cggcgattga acgacatcgt aaaggatcaa ttttaaggag acaaaatcca
    13921 tggatataaa ccgggagaaa gccctcgatc tagcagtcag ccaaatcgaa aaacaattcg
    13981 gtaagggcgc gatcatgaag cttggcgagg gaggtgtgac aaaagacgtt ccggtgatct
    14041 cgaccggctc tcttgggctc gatatcgctc tcggtatcgg cggcgtgccg cgcggccgag
    14101 taatcgaaat atatggccct gaatcatccg gtaaaacgac attggcgtta cagatcgttg
    14161 ccgaagcgca gaagcaaggc ggcatgggag cgtacgtcga tgccgagcat gccttggatt
    14221 tgggttacgc taaaaagctc ggagtcaaga cggacgattt gttagtttca caaccggacc
    14281 acggcgaaca agccttggaa atcgccgaga ctctggtccg tagcggcgcc atcgatgtcc
    14341 tggtgatcga ctcagttgcc gcactcgtcc ccaaagcgga aattgaaggt gagatgggtg
    14401 attcgcacat ggggttgcag gcgcgactga tgtcccaggc cttgcgcaaa ctcaccggaa
    14461 ccatcgcgcg ctcccacacc gttgtcatct ttatcaacca gatccgtatg aagatcggtg
    14521 tgatgttcgg caatccggaa accactaccg gcggcaatgc tctaaagttt tatgcgtcgg
    14581 tgcggatgga tattcgccgg attggcgcgc tgaaagacgg tgataatatt gttggtgggc
    14641 gcacccgggt aaaggtagtt aaaaataaga tggcgccgcc attcaaagaa gccgagttcg
    14701 atgtgcttta cggcactggc atttctcgcg acggggagat tgtcgatctc ggcagcgaaa
    14761 tgggcatcgt cgagaaaagc ggggcctggt attcgttcgc cggcgataga atcggccagg
    14821 gacgcgaggc ggcgaaaaat tttctgaaag agcatcctga gatcgctcag caaattatgt
    14881 caagggtgat ggagcgtgtc ggcttgaaac gagaggaagc tcctgttacg acgggaaatg
    14941 tagacaggaa agaaaaagcg aaagtaaagt gacaaaacgt tagtcgcgga tctgaaccga
    15001 acgagtcgca cacccaaatc gcctgagtcg gaggaggagg cgcttcaatc cgcccttcga
    15061 tatctcagct accgcgcccg cagtgaagcg gagctactcg cttatctatc caggaaaggc
    15121 tattcagccg ccgcccgcga gaaggctctg ggaaaactcc gctctctcaa ttacattaat
    15181 gatgagtcct ttgcgcgcaa ttgggcctcg tcccgagccg ccggccggaa ctacggaccc
    15241 aagagaattg agcaggaact aaaggcaaag ggtattgggc agccgttgat ccgggatgta
    15301 atcggcgcga tcttctctca gggagacgaa gaaacgaacg ccaaaaactt gctggagaaa
    15361 aaatttaaag gagtgagttt tcgagatccc aaagtccttc gtcgggcgag cgcgtttctc
    15421 gcacgccgtg gttatagcaa tcaggttatt tttagtttga tgcggcgccg cccggaggac
    15481 gatttagagc catgatgact ggaaacgata ttcgcagatc cttcttggat tacttcacga
    15541 agcaatctca tacagttgtg aaaagttctt ctctggtgcc cgagaaggat ccaacattgc
    15601 tcttcaccaa tgccggcatg gtgcagttta agaacgtttt tcttggccag gaacggctgt
    15661 cctattcgcg cgccacaagc tcgcagaagt gtttgcgcat cagcggcaaa cataacgacc
    15721 tggaggcggt cgggagggat acctatcatc acacgttctt tgagatgttg ggcaattggt
    15781 ccttcggcga ttattataag gccgaagcga ttgaatgggc ctgggagctc ctgacccggg
    15841 agtggagttt gcccaaagag aagctctggg cgaccgtata cctcgacgac gatgaagcgg
    15901 agcgtctttg gcccaggatt agcgggctcc cggctgagcg cattcggcgc tttggtgaga
    15961 aggaaaattt ctgggagatg ggcgagaccg gaccctgcgg gccctgtagc gagatccatc
    16021 tcgaccgcgg acctgaagct tgcgacagga agggcctgcc ggggcatgtc tgccaagtca
    16081 acggcgattg cgcccgctac attgaactat ggaatcttgt ttttattcag tacaaccgca
    16141 aagagaaccg cgagctggaa gagctgccat ccaaacatgt tgataccggc atgggcttgg
    16201 aacgcattac tgcggtgatg cagaatgtac tatcgaatta cgatatcgat tacatgcgcg
    16261 agctgacggc ggcggtcgaa aatttggccg gcaaacgtta cggcgctgat gcgacggccg
    16321 atatttcctt tcgtgtgatt gacgatcacg ccagagctgt cagctttctc attgccgatg
    16381 gtgtgatgcc tagcaatgat gggcgtggct atgtcttacg acgattgctt agaagggcag
    16441 cccgtcacgg ccgcctgatc ggattgaagg aaccgtttct ttatcaaatc gcgagcacgg
    16501 tcggaaaagt gatgggggag gcgtatcctg agcttcaaac tgaagcaccg cgcatccgtg
    16561 agatcatccg cagtgaggaa gagcgcttca gtgaaacgtt ggagaaggga cttgtcctcc
    16621 tcgaggacgc tgccgcaaaa ctgaaacagg aaaacaagaa gatgctgccg ggcgaggttg
    16681 cttttcgcct gtacgacact tacgggttcc ctctcgacct gactgaggac attcttcgcg
    16741 ccgagaatat ttctgtcgat cagcacggtt tcgaaaatct catggacaca caacgttccc
    16801 ggggtcgtga agcccgggcg attgcgatgg acgtttttga ccaggttgcg gttgcggaca
    16861 cggtgatagc aactaaattc atcggctacg atcaactcga ggcagagtcg ac
//
LOCUS       DASUVS010000214        15652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_960733, whole genome shotgun sequence.
ACCESSION   DASUVS010000214 DASUVS010000000
VERSION     DASUVS010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 15652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15652
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960733"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="VF182_12080"
     CDS             <1..1042
                     /locus_tag="VF182_12080"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6437864.1"
                     /translation="RKGSVIMAHNFEVIDADGHITEEDNQLKQYMDARYRDRAATLTP
                     RDSWDRSIGGTLGTRARDARSWLDAMDRGGVSTAVLYPTNGLSVGWIREPDVAVAYSK
                     AWNDFASEEFQKVSPRLKAVALVPFQDVPEAVKELRRAVTELKLPGLMLPAVGLRLPL
                     GHESFWPIYEEAERLDCMVGVHATVRGPHYFAADMFDQFIEVHTLSHSFAQMMQCTSM
                     MFRGVFDQFPKLRVAYMEAGCSWAPYWFGRMNEEWEKRGVVEAPKCKKKPTEYFKEGR
                     IYFHAEDYEPLIGATTEVISHNVIYYASDYPHWDTEFPENIDHLARRKDLNDEAKKWL
                     LAETAKKLYNLS"
     gene            1052..2077
                     /locus_tag="VF182_12085"
     CDS             1052..2077
                     /locus_tag="VF182_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6437865.1"
                     /translation="MATRRKVCFTGPTRLKVAEEILRGAGCELVLGKNQDDFREYKYP
                     RRELVELIGDSAVVFPSGRDIIGGEILDSCANLQAVVKSSIGIETVDVDAATDIGILC
                     CNSPTPENYLGVAEATVGLMVMLFKRLKFNEALLRQGGWKEPQNRGMLMIGRTIGIIG
                     VGRIGRNVAKRLSGWDLNFLGYDPYVDQETAAPLGIKMVSLEQLLGESDLVTIHVVLT
                     RETRKMIQLPQLKMMKKTAYIVNTSRGQVFNEADLAQALNEGIIAGAALDVFDEEPTP
                     LDNPLRQVDPTKLTMTPHIIGNNPGSLEAGQRLAAQSILSILDGKPPDTVVNPKAIPL
                     WKERFWP"
     gene            2189..3208
                     /locus_tag="VF182_12090"
     CDS             2189..3208
                     /locus_tag="VF182_12090"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437866.1"
                     /translation="MRESKRLTLALALLAVLGLGKIPARAASAPDQMVGIHSSRVMSQ
                     SLPWMAQEAGLFKKYNLDFNLVFISSSSIVTAALLGGDAEMTVTGGIGNVNAYVRGST
                     DVVFIGAIKNVMTQTIVAGGNIKRPEDLKGKRIGVSRIGGNSHYFTLQALRKFNMDPN
                     RDVSFMQTGGDPETFAALISGSLEVANLTPPTDALAISRGYHYVIYGPDLKIPYAATA
                     FVTKRSVIAKRPQVVGNFMRAMAETAKIMHTDREFVYKVLGKYLRITDRSVLDSAYNA
                     EIKALEPRLAIKTEALQAILDEVAQTDPRAKKVKPQELIDSRYLDEMEKSGFMEQLWG
                     KKKEL"
     gene            3205..4200
                     /locus_tag="VF182_12095"
     CDS             3205..4200
                     /locus_tag="VF182_12095"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437867.1"
                     /translation="MMRNFFPAIYFLLVAPVWTHAASAPDKLVGIHSARVMSQSLPWM
                     ADEAGLFKKYNIDFNLVFISSSSIVTAALLGGDAEMTLTGGIGNVIAYVRGSTDVVFV
                     GGVKNVMTQSLVAGGALKRPEDLKGKRIAVSRIGGNTHYFTIQALRRHGLEPNRDFSF
                     MQSGGDPESLAALMTGNVEAAALTPPSDAKALANKFHYVLYGPDLKIPYAATAFVTKR
                     SVLTKRPQVIGNFMRAMAETAKLMQTDREFVYKVLGKYLRVTEKPILDAAYNAEIKAL
                     EPRLAIKAEAIQATLDEIAPNDPRAKKISPEELIDRRYLDEMEKTGFFDQLWAKK"
     gene            4215..5375
                     /locus_tag="VF182_12100"
     CDS             4215..5375
                     /locus_tag="VF182_12100"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6437868.1"
                     /translation="MEQTIISSDSHVIEVPDLWEKGVPSSFAERAPKAFFDEKRDAWM
                     FGSAEVPVQAVGGLFMAGQRPDQVESFRRAGFSVARPGGWDPLERMKDMVTDGVAAEV
                     LYPSLGLGLFCLEDAALQEALFRTYNDWLIDYCQKVPDRLFGIALISMFDIDHAIAEM
                     KRCKEQGIVGTMIWQVPHPKLPFSAEHYERFWAASQDLELPVHLHILTGFGDSMHRQT
                     SHGIQRYRIGVQQTREIEDALFDIIFTGVLERYPQLKIVSVENEIGWMPFWLGQCDKA
                     FKRHRHSEKLTIDKPPSEYFRRQVYATFFNDPVGAKLFSWWGCDNFMWSNDYPHQNST
                     WPNSRDVIARDMGHLDAAARQKLLSSNVTKLYQIKVADSLPDTRVGATAAHA"
     gene            5392..6411
                     /locus_tag="VF182_12105"
     CDS             5392..6411
                     /locus_tag="VF182_12105"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6437869.1"
                     /translation="MELREAVKVLDSDAHARDLDENLRPYLNEPYRSQRAPFFLRETY
                     DRNLGGTLGFSGAKHEERLAAMDTQQIDSAVMYPTSGLGIGAIRDPAFNAALCRAYND
                     YIADYCKTSPRLKAVANLPVNNPAEAANELNRAVTKLGLCGGMLAAQSHMKNLGSPEF
                     YPLYEEAQRLNTPIAIHAFGGDEPGTEIFDKFICLHTTGHPFPVLRQLTAIVFGGIPE
                     LFPKLKIGYLEVGCGWIPYWAERMDEEWEKRGKAEAPLCKKKPSEYLTNGNSYYGCEP
                     EEKMMGYVVNEIGSKTLMYASDYPHWDMSWPESATIVWNREDLSVEAKKDILLNNAKR
                     FYNLS"
     gene            6490..8223
                     /locus_tag="VF182_12110"
     CDS             6490..8223
                     /locus_tag="VF182_12110"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6437870.1"
                     /translation="MIQTKGLTGAQSMLRVLARMGVEKIFASPGSEWSPVWEALAEPG
                     VEGMPQYLSTRHEEVAVGMASGYAKATGKLPAVMIHTTVGSLHATMGMRGAIREQVPM
                     VVFSGESIGYGEDEGPDPGGQWLGHLSNIGGPAKMVEQTVKWSFGVNNKAILPATIQR
                     ACQLAMAAPKGPTFVSLSMEHLFDKMIKDVPSVGFAPGATADPKGIEELAKLLAGAKN
                     PVIVTEDSGRTAKSVHCLVEIAELLGCVVVETRSTGALNFPRTHPLHSGYDPRKALAG
                     ADAILLLAVIAPWHPASATPSPGATVAILDENPLRHDMPYYGYQADLCLTGEIESSLE
                     QLLAALKSKVSLSDPSRKQRAEEIGKKNNAQRKAWRDEALALADQKPMDTRWVAHEVS
                     QVLPADAMVVEETITHRLAIHRYFDALKPGSFFAGCVGGLGTGLSTALGVKSANPKRP
                     VVCVIGDGSFNYDPAPAVYGAAQEHNLPFLTVMFNNQGYLSQKSGIPKYYPDGWAVKS
                     KNFSGLHIAPCPEYAQIIKAFDGYGEKVEDPADVRKAVERGLRAVAGGQVALLDIRLK
                     PVEQIVERGEG"
     gene            8333..9340
                     /locus_tag="VF182_12115"
     CDS             8333..9340
                     /locus_tag="VF182_12115"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437871.1"
                     /translation="MHARGIPFEKRWRVSVAVFLSLVAFGTGQAWSADKLIGIHSARV
                     LSQAMPWIAQDAGLFKKYNLDFPMVFIPASSAVTAAMLGGDGEIALTGGEGMIRANLA
                     GGSDFVFIGAVKNILTHSILGKPELKTPEDLKGKKVGVTRLGGNPHYFAIQALRLKGL
                     DPSKDVTFIQTGGAPETLAALKSGVIDAAALTAPTDTQATAMGYNFVIFGPDLKIPYA
                     ATVFVTRRAVIAKRSAVLGQFMRAMAEASRILHNDREFVYKVLGKQLRLTDRKILDAA
                     YNGEIKALEPRLSLRAEPYQAILDDIAKSDPRAKKVKAEEMMDSRYVDELEKSGAFDK
                     S"
     gene            9366..10076
                     /locus_tag="VF182_12120"
     CDS             9366..10076
                     /locus_tag="VF182_12120"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6437872.1"
                     /translation="MENSFHDRLVATRDRKHSKNHPFFELWAQGKLTREQTAFYCTQH
                     YHFVGEFLNWLAYQGSQIRERDVKNYLFENLGDEENPDDRHLDMLKDYVAASGVNRDS
                     VESSVVLAATEGLQNWGWRMVYQRPWQVALAGMFIGLESQFLDICKKIVPALHKHYGY
                     APHAREIRFFEEHIYADEIHGSKGFAIVEKYCNTPELQALAIQTVEDATVRRWRYMNG
                     IYWYAIHGKQDDTPSLPG"
     gene            10344..11792
                     /locus_tag="VF182_12125"
     CDS             10344..11792
                     /locus_tag="VF182_12125"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6437873.1"
                     /translation="MKIRIDGGVVVGWSGTGHEIIPDGSVVIENDTIRSVGTDKSPAV
                     DKVIDASGKIVSPGFVNLHVHSQLNVGDYLLPDVTRKDYLTANWFVFGAPLKEKVTPP
                     PAPAVAIGRKYALFSALKNGATTVLDPGGGPGALDEYVAIVGQQGGRVFFSPPYRSSD
                     IFTDAQGRLYYEEREDKGRPGLERAVAFIKQYHGAHNGRIQGILNPAQAETCEPALLK
                     ETAAAAKELNVPIHLHAGGNAREFLHILNTYRKPVVEYLAGTGILGPRTTLGHMAITG
                     GHSLIDYPRGEELKLIAESGATVGHCPHKCAKMAFAMESFDQYLTAGVRIGLGTDTYP
                     LDIIAEMRYASLISRLVDRNASGARPADCFNAATIGGADALGRKDLGRLAAGAKADMV
                     IINMRTTRYGPWRDPINALVEYGSGADVETVIVDGQIVIDNGRSTRIDEDELFAQANA
                     GAQRAWDGWANRDWNGRSAEDIIPPAFPTRSK"
     gene            complement(11920..12393)
                     /gene="bfr"
                     /locus_tag="VF182_12130"
     CDS             complement(11920..12393)
                     /gene="bfr"
                     /locus_tag="VF182_12130"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEX6437874.1"
                     /translation="MKGDEKVVSVLNQVLRKELTGINQYFIHAKMCKNWGYERLYKTM
                     WDESIGEMKHADEVIERLIFLEAIPNMTGYDKISVGSNVKQQLENDMGLELAALTILR
                     PGIRTCLEAADDASRELLEHILVDEEKHVDWIEAQLHQINEIGTQNYLAQQIKKD"
     gene            complement(12452..12835)
                     /locus_tag="VF182_12135"
     CDS             complement(12452..12835)
                     /locus_tag="VF182_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437875.1"
                     /translation="MTASSRILFLSAMVLIFAGCTRSYDEKIAGVEIPIPRGMKKSDG
                     AGIEVALLGFGGGQSSFRGQLEPRTVIEFYKKEMPARGWQPNVGVISKGGMLTYAKDA
                     RTVMIMVGGNNDATTMTVTVGGTGR"
     gene            complement(12930..14105)
                     /locus_tag="VF182_12140"
     CDS             complement(12930..14105)
                     /locus_tag="VF182_12140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6437876.1"
                     /translation="MADNYHSFSRAEIGPVIVVSLISALRLLGIFLILPVFSVYAVHY
                     PEASLAMAGIAFGAYALTQSILQIPLGWASDRWGRKPVLLIGLALFGAGSIGCGLAQS
                     ISQLIIARAIQGTGAIGSVALAALADLTRPAVRTQAFTITGIAIGSAFMIGILGGPLL
                     AAAIGLSGLFYVLAGLAFAAMILAAISFPKENRSIDKESAMAFRPILMHGELRPIFIA
                     AFVLSFTLNLFFFTYPLSWQEIGLNRAELWKVYVIIFLPSALLVFPYMRRAERLGRFR
                     VPIMIGWLTAAIGYAVYLVGAQSDLLLYVSGAAFFFGYSLFQPLLPTFLTKRIPQGGR
                     GTATGVYTFFGFIGSSLGGMLGGVLIHISPALPEFLGVTLLVLWYLTGLPTAPETAS"
     gene            complement(14220..15137)
                     /locus_tag="VF182_12145"
     CDS             complement(14220..15137)
                     /locus_tag="VF182_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX6437877.1"
                     /translation="MFGTSYEAYVPKLIDFSINFAQSSLRVVLIVIAALLAIKFIRIG
                     LSRLEAILVRARESSESVPGAAAMRIRTLMSVLWTIVAGLVWFTAVLTILGQVGVNVT
                     PILASAGVVGLAVGFGAQNLVKDFVSGFFLILENQIRVGDVAVINGTGGLVEAITFRT
                     IVLRDQAAVVHVFPNGSINTLSNMTKDWSAYVIDVGVSYREDTDRVVEVMRRVAGELQ
                     SDPQYGSAMLAPIEIIGVNAFTDSAVTIKARLKTLPIKQWMVGREYRRRLKKAFDAEN
                     IEFPFPQRTVLVGEAKAPLPLGIKPSEGN"
     gene            complement(15181..>15652)
                     /locus_tag="VF182_12150"
     CDS             complement(15181..>15652)
                     /locus_tag="VF182_12150"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX6437878.1"
                     /translation="LEYFLAAAREQKKVVNLHTKGGERSILELLERYDVRRPIIHWYS
                     GPLDILGALVQFGAYFTVGVEVLYSETIQAIARAIPDRLLLTETDNPGGLKWLKGIVG
                     MPSDIKKVIDELARIKSSTPESMSRLVHTNFVKLIQNDPWLPQVQALLSQPYNT"
BASE COUNT         3592 a         4258 c         4483 g         3319 t
ORIGIN      
        1 aagaaagggg tcggtcatta tggcgcacaa ttttgaagtc attgatgctg acggccacat
       61 cactgaagag gataaccagc tcaaacagta tatggacgcg cgttatcgcg atcgcgccgc
      121 taccctgacg ccgcgcgata gctgggatcg ttcgatcggc ggaacattag ggactcgcgc
      181 gagagacgcg agaagctggc tcgatgcgat ggatcggggc ggtgtttcga cggcggtgct
      241 ttatccgacc aatggtctca gcgtcggttg gatccgcgag cccgatgtgg cggtggcata
      301 ttccaaggca tggaacgact ttgcttcgga agagtttcag aaagtcagcc caaggcttaa
      361 ggcggtggcg ctggtcccgt ttcaagatgt gcccgaagcg gtcaaggagc tgcgccgcgc
      421 ggtcacggaa ctcaagttgc cgggtctcat gctgccggca gtagggttga gactgccatt
      481 ggggcacgag agcttctggc ccatttacga agaagcggag aggctcgatt gcatggtcgg
      541 ggttcacgcc accgtgcgcg ggccgcatta tttcgccgcc gacatgttcg atcagttcat
      601 cgaagttcat acgctatctc actcctttgc tcagatgatg cagtgcacga gcatgatgtt
      661 ccgcggcgtt tttgatcaat tccccaaact ccgggttgct tatatggagg cgggttgtag
      721 ctgggcgccc tactggttcg ggcgaatgaa tgaggaatgg gaaaagcgcg gcgtggtgga
      781 agctcccaaa tgcaagaaga aaccgacgga atacttcaag gaagggcgga tctacttcca
      841 tgccgaggac tacgagccgt tgatcggggc caccaccgag gtgatatcgc acaatgttat
      901 ttattatgct tcggactacc cgcattggga caccgagttt cctgaaaata tcgatcacct
      961 cgccagacga aaagatctca atgacgaggc caagaaatgg ctgctcgccg agaccgcgaa
     1021 aaaactttac aacctcagct gaggccgacc tatggcaaca agacgtaaag tttgctttac
     1081 cggtccgaca cggctcaagg tcgccgaaga aattttgcgc ggcgccggtt gtgaactggt
     1141 gttggggaaa aaccaggatg actttcgcga gtacaaatat ccgagacgcg agctggtcga
     1201 actgattggt gactccgcgg tggtgtttcc ctccggtcgc gatatcatcg gcggcgagat
     1261 tctcgactcg tgcgccaacc tgcaagccgt ggtcaagtcg agcatcggca tcgaaaccgt
     1321 tgatgtcgat gctgctaccg atatcggcat tctctgctgc aacagcccga cgccggaaaa
     1381 ttacctcggg gttgccgagg ctacggtggg attgatggtg atgcttttta aacgcctcaa
     1441 attcaacgaa gctttgctgc gccagggcgg gtggaaggag ccgcagaacc gcggcatgct
     1501 gatgatcggc cggaccattg ggatcatcgg cgtcggccgc attgggcgca acgtcgccaa
     1561 acgcttgagc ggctgggatc tgaactttct cggttatgat ccgtatgtcg atcaggaaac
     1621 ggcggctcct ctcggcatca agatggtctc attggagcag ttgctcggag aatcggatct
     1681 ggtgacgatt catgtcgttc tgacccggga aacgcgaaag atgattcaat tgccgcagct
     1741 caagatgatg aagaagaccg cgtacatcgt caatacgtcc cgcggtcaag tgttcaacga
     1801 ggccgatctg gcgcaagctc ttaatgaagg gatcatcgcc ggggcggcgt tggatgtttt
     1861 cgatgaagaa ccgacgccgc tggataatcc cctgcgccag gtcgatccca ccaagctgac
     1921 catgacgccg cacatcatcg gcaacaatcc gggctccctg gaagccggtc agcggctcgc
     1981 cgcgcaaagt attctttcga ttctcgacgg caagccgcct gacaccgtcg tgaatcccaa
     2041 agcgatcccg ctgtggaaag agcgcttttg gccgtgaacc tctgactgac ttatcttttg
     2101 acaaccggtt cattgaagcg atagaaaaag gctgggcgag agatcacgct tggaagtgtt
     2161 tgccgccgtt cgcagcagga gaataccgat gagagaaagc aaaagactga ccctcgcatt
     2221 ggcgctcctg gcagtgctgg ggttaggcaa gatcccggcg cgcgccgctt cagctcccga
     2281 tcagatggtc ggtattcact cctcgcgagt catgtcccaa tcgctcccct ggatggcgca
     2341 ggaagccggg ctatttaaga agtacaatct cgatttcaac ctggtattta tttcatcctc
     2401 gagcattgtc accgccgcgc tgctcggcgg cgacgccgag atgacggtca ccggcggcat
     2461 cggcaatgtc aacgcttacg tgcgcggttc caccgatgtg gtcttcatcg gcgcgatcaa
     2521 gaacgttatg acccaaacca tcgtcgccgg cggcaacatc aaaagaccgg aagacctgaa
     2581 ggggaagaga atcggcgtga gccgcatcgg cggcaattcc cattatttca cccttcaagc
     2641 gctgcgcaaa ttcaacatgg atccgaaccg cgatgtttct ttcatgcaga ccggcggcga
     2701 tccggagacc tttgcggcat tgataagcgg gtcgcttgaa gttgccaatt tgacgccgcc
     2761 gaccgatgcg cttgcgatct cacgcggcta tcactatgtg atctatggtc ccgatctgaa
     2821 gattccttac gccgccactg cttttgttac caaacgttcc gtgatcgcaa agcggccgca
     2881 ggtggtggga aattttatgc gggccatggc ggagacggcc aagatcatgc acaccgaccg
     2941 ggaatttgtt tacaaggtgc ttggcaagta cctgcgaatt accgatagaa gcgtgctcga
     3001 ctctgcttac aatgccgaga tcaaagcgct cgagccgcgg cttgcgatca agaccgaagc
     3061 gttgcaagcg attcttgacg aagtggccca aaccgatccg cgggcaaaga aggtcaaacc
     3121 gcaagagctc atcgacagtc gttacctgga cgaaatggaa aaaagcggat tcatggagca
     3181 actctggggg aagaagaagg agctatgatg cgaaattttt ttcccgcgat ttactttctg
     3241 cttgtcgcgc cggtttggac tcatgccgct tcggcccctg acaagctggt gggtatccac
     3301 tctgcgcggg tgatgtcgca gtccttgccg tggatggccg atgaggccgg cctgttcaaa
     3361 aaatacaata tcgatttcaa cctggtattt atttcttcct cgagcatcgt cacggcggcc
     3421 ttgctcggcg gcgatgctga gatgactttg accggcggta tcggtaacgt gatcgcttac
     3481 gtccggggtt cgacggatgt ggttttcgtc ggcggggtca aaaacgtcat gacccagagt
     3541 ctggtcgcag gaggagctct caaacgaccg gaggatctca aagggaagag gatcgcggtc
     3601 agccggattg gcggcaacac gcattatttt accatccagg cgctgcggcg gcacggcctc
     3661 gaacccaatc gggatttcag cttcatgcaa agcggcggcg atcccgagtc gctcgcggct
     3721 ttgatgaccg gcaacgttga ggcagccgcg ctcaccccgc cgtccgacgc caaagctctc
     3781 gcaaataaat tccattatgt actttacggg cccgatttga agatcccgta tgcggccacg
     3841 gcgttcgtga ccaagcgttc ggtgctcacc aaacgcccgc aggtcattgg taatttcatg
     3901 cgcgccatgg ccgaaaccgc caagctcatg cagaccgacc gggagtttgt ttacaaggtg
     3961 ctcggcaaat atttacgcgt gaccgaaaaa ccgatcctgg atgccgccta caacgccgaa
     4021 atcaaggcgc tcgaaccgcg gctggcgatc aaggcggaag ccatccaagc gactctcgat
     4081 gaaatcgccc cgaatgaccc gcgcgccaaa aaaatatcac cggaagagct catcgatcgg
     4141 cgttatcttg atgaaatgga aaagaccggg ttttttgatc agctatgggc caaaaaatag
     4201 ttcgaggagg agccatggaa caaacgatca tctcgtcgga ttctcatgtc atcgaagttc
     4261 cggatttgtg ggagaagggt gtgccgtcat cgtttgccga gcgcgcgccg aaagcgtttt
     4321 tcgatgaaaa gcgcgacgcc tggatgttcg gctcggctga ggtgcccgtg caagcagtgg
     4381 gcggcttgtt catggccggc cagcgcccgg accaggtaga gagttttcgc cgcgccggtt
     4441 tttccgtcgc ccggcccggc ggctgggatc cgctcgagcg gatgaaagac atggtcacag
     4501 acggtgtcgc cgctgaagtc ctttatccca gtctcggtct cggtttgttc tgcctcgaag
     4561 atgccgcgct tcaggaggcg ctgtttagaa cctacaacga ttggttgatc gattattgcc
     4621 agaaagtgcc ggaccggctc tttggtattg cgcttatttc catgttcgac atcgatcacg
     4681 ccattgccga aatgaagcgc tgcaaggagc aggggattgt cggcaccatg atctggcaag
     4741 tgccgcatcc caagctgcct tttagcgccg aacactatga acgattctgg gcggcatccc
     4801 aggatctgga attgccggtt catctgcaca tcctgaccgg tttcggcgac agcatgcatc
     4861 gccagaccag tcacggcatc cagcgctatc gtatcggcgt gcaacagact cgagaaatcg
     4921 aggacgctct gttcgacatc attttcaccg gagtattgga acgttatccg cagcttaaaa
     4981 tcgtttcggt ggagaatgaa attggctgga tgccgttctg gctggggcaa tgcgacaaag
     5041 ccttcaagcg ccatcgacac tcggagaagc tgacgatcga taagccgccc agcgaatatt
     5101 ttcgccggca ggtttatgcg accttcttca acgatccggt cggcgccaaa ctgttttcat
     5161 ggtggggatg cgacaacttc atgtggtcca atgactaccc acatcaaaat tccacctggc
     5221 ccaactcgcg ggatgtgatt gcgcgtgata tgggtcatct cgatgctgcc gcccggcaga
     5281 aactgctcag cagcaacgtc actaagcttt atcaaataaa agtagcggat tcgttacccg
     5341 atacccgtgt gggcgcgacg gcagcgcacg cctgaatgag gaggagcaat catggaattg
     5401 agagaagcgg tgaaagtcct ggattccgac gctcatgcgc gggatctcga tgaaaatctt
     5461 cggccctatc tgaacgaacc ctaccggagc caaagagcgc cattttttct ccgagaaact
     5521 tacgaccgca atctaggcgg gacgctaggg ttttccgggg cgaaacacga agaacgtctc
     5581 gcagccatgg acacgcagca aatcgatagc gcggtcatgt atccgacaag cgggttgggt
     5641 atcggcgcga tccgcgatcc ggcgttcaat gcggcgctgt gccgggccta taacgattat
     5701 attgcggatt actgcaagac gtcgccgcgt ctgaaagcgg tggccaacct cccggtcaac
     5761 aatcccgccg aagcggccaa cgagctcaat cgcgcggtga caaaactcgg gctctgcggc
     5821 ggcatgttgg cggcgcagtc tcatatgaaa aatcttggca gccccgagtt ctacccgctc
     5881 tatgaagagg cgcagcggct caatacgccg attgcgattc atgctttcgg cggcgacgag
     5941 cccggcaccg agattttcga taagttcatc tgcctgcaca ccaccggcca cccgtttccg
     6001 gtgctgcgcc agctgacggc aatagtcttc ggcggtattc ccgaactgtt ccccaaacta
     6061 aagatcggct atctcgaagt cggatgcggc tggataccgt attgggcgga gcggatggat
     6121 gaagagtggg aaaaacgcgg caaggccgaa gctccgctgt gcaagaaaaa accgagcgag
     6181 tatctgacca acggcaacag ctattatggt tgcgagcccg aagagaagat gatgggctac
     6241 gtggtcaacg agatcggctc gaagacattg atgtatgcct ccgattatcc gcactgggat
     6301 atgagttggc ctgaatcggc gaccattgtg tggaaccgtg aggacctgtc ggtggaagct
     6361 aaaaaggata ttctgcttaa taacgccaag cgattttaca atctttcgta gcgcttaaag
     6421 cggccgtcgg tcttgagtat ttctgctgcg gaccggcggc gatttcttca tttaccgaaa
     6481 ggaagacata tgattcaaac taaagggttg accggggcgc aatccatgct gcgcgtcctc
     6541 gcaagaatgg gcgtggagaa aatttttgcg tcgccggggt ccgaatggtc gccggtgtgg
     6601 gaggcgctcg cggaaccggg cgtcgaaggc atgccgcagt atctgagcac gcgccacgaa
     6661 gaagtagccg tcggcatggc aagcggatac gcgaaggcca ccggcaaact gccggcggtc
     6721 atgatccaca ccacagtggg ctcgctgcac gcgaccatgg gcatgcgcgg cgcgattcgc
     6781 gaacaggtgc cgatggtagt cttcagcggt gaatcgattg gctatggcga agacgaagga
     6841 ccggatccgg gagggcagtg gttgggccat ctctccaata tcggcggccc ggcgaagatg
     6901 gtcgagcaaa cggtgaaatg gagtttcggt gttaacaaca aagcgatttt gccggcgaca
     6961 attcagcgcg cctgtcaact cgcaatggcc gcgcccaagg ggccgacgtt cgtatctttg
     7021 tcgatggagc atctgttcga caagatgatc aaagacgttc cctcggttgg cttcgcgccc
     7081 ggagcgaccg ccgatccgaa gggcatcgag gagctcgcca agctgctagc cggcgcgaaa
     7141 aacccggtca ttgtcaccga agattcgggg cgcacggcaa agtccgtcca ttgcctggtc
     7201 gagattgccg agcttctggg gtgtgtggtt gtcgaaaccc gcagcacggg cgcgcttaat
     7261 tttccgcgca ctcatccgct gcacagcggc tatgatccac gcaaagcttt agcgggcgcg
     7321 gacgcgattc tcttgctggc ggtaatcgcg ccgtggcatc cggcttcggc gacgccgtcg
     7381 cccggcgcca ccgtggcgat tctggatgag aatccgttgc ggcatgatat gccctactat
     7441 ggttatcagg ccgatctctg tttgaccggt gagatcgaaa gctcactcga gcagctcttg
     7501 gccgcattga agagtaaagt ttccctcagc gacccatcac gcaagcaacg cgcagaggag
     7561 atcggcaaga aaaacaatgc ccaaaggaaa gcctggcgcg acgaggcgct ggcccttgcc
     7621 gatcagaaac cgatggatac ccgctgggta gcgcatgaag tcagccaggt cttgccggcc
     7681 gacgccatgg tcgtggaaga gacgatcacg caccgtttgg cgattcatcg ttatttcgat
     7741 gcgctcaagc caggctcttt tttcgcaggc tgcgtcggcg gcctgggcac cggcttgtcg
     7801 acggcgctgg gcgtcaaatc ggccaatccc aaacgtcctg tggtgtgcgt gatcggtgac
     7861 ggatcgttca actatgatcc ggcgcctgcg gtgtacgggg ccgcccagga gcataacctg
     7921 ccgttcctca ccgtcatgtt taacaaccag ggttatctgt cgcagaaatc cggtatccca
     7981 aaatattacc ctgacggttg ggcggtcaaa tcgaagaatt tctccggcct gcacatcgcg
     8041 ccgtgtccgg agtatgcgca gatcatcaaa gctttcgacg gctatggcga aaaggtcgaa
     8101 gaccccgccg atgtgcgcaa agcagtcgag cgcggtctac gcgccgtggc cggcggccag
     8161 gtcgccttgc tcgacattcg acttaaaccg gtagagcaga tcgtcgagcg cggcgaggga
     8221 taatctctgg agcacaacca atagtaccac ggagctagat gctatcatta gagcgatgtc
     8281 cggcgtaaca agccgacatc gctcttgtgg tttagaggca cagaggtgcg aaatgcatgc
     8341 gcgcggaatt ccatttgaga aacgctggcg agtgagtgtt gcggtttttc tatcccttgt
     8401 agcgttcggc acgggtcagg cgtggagcgc tgataaactc atcggcatcc attcggcgcg
     8461 agtcttatct caagcgatgc cttggatcgc tcaggatgcc ggtctcttta aaaaatacaa
     8521 tctggacttt cccatggtat ttattcctgc gtcgtccgcg gtgaccgcgg ctatgttggg
     8581 cggcgacggg gagatagcgc tcaccggagg cgaggggatg attcgcgcca acctggcggg
     8641 tggcagcgac ttcgtgttta tcggagcggt aaaaaacatc ttgactcata gcatcctggg
     8701 caaaccggag ctcaagacgc ccgaggatct gaaagggaaa aaagtcggag taactcgact
     8761 cggcggcaat cctcattatt tcgccattca ggccttgcga ttgaaaggat tggacccaag
     8821 caaggacgtc actttcattc aaaccggcgg cgcgccggaa acgctggcgg ccttgaaatc
     8881 gggagtgatc gatgcggcag ccttgaccgc gcccacagac acgcaagcga cagccatggg
     8941 ttacaacttc gtcatcttcg gccccgatct gaagataccg tacgcggcaa cggttttcgt
     9001 gacccgtcgc gccgtcatcg ccaagcgttc cgcagtgttg ggtcaattca tgcgcgcgat
     9061 ggcggaagct tccaggattc tccacaatga ccgtgagttc gtctataagg ttttaggcaa
     9121 gcaactccgc ctgacggaca gaaagatttt ggatgccgcg tataacggcg agatcaaggc
     9181 gctcgaaccg cgactctctc tccgagccga accgtatcag gcgattctgg acgacatcgc
     9241 caagagcgat ccacgagcca agaaagttaa agctgaggag atgatggaca gtcgttatgt
     9301 cgatgagcta gaaaaaagcg gcgctttcga taaatcttag acttgtaaac cgttgcgagg
     9361 atcaaatgga aaacagcttt catgatcgat tagtcgcgac ccgcgatcgc aagcacagta
     9421 agaatcatcc gtttttcgag ctatgggcgc aagggaagct gacccgggag caaaccgcct
     9481 tctactgcac gcagcactat cattttgtcg gcgagtttct gaactggctt gcgtatcagg
     9541 gctcgcagat ccgcgagcgt gatgtaaaaa attatctgtt cgagaatctc ggggatgaag
     9601 agaatcccga cgatcgtcat ttggacatgc tcaaggacta cgtcgccgct agcggcgtca
     9661 atcgggacag cgtcgaaagc tccgtcgttc tcgccgccac cgaaggattg cagaattggg
     9721 gctggcggat ggtttatcaa cggccttggc aggtcgctct ggccgggatg ttcatcggtc
     9781 tcgaatcgca gtttttggat atctgcaaaa agatcgttcc cgctctacac aagcattatg
     9841 gttatgcgcc gcatgcgcgg gagatccgat ttttcgaaga gcatatctat gccgacgaga
     9901 tccatggctc caaaggattt gccatcgtcg agaagtattg caacacgcct gagctgcagg
     9961 ctctggcgat ccagacggta gaggatgcga ccgtccggcg atggcgctat atgaacggaa
    10021 tctactggta cgcaatacat ggtaaacagg acgatacgcc gagcctgccg ggctaaggtc
    10081 tagcagcccc ggatttcgca attgaaaggg gacggcggga tcaagggacg agccctccgg
    10141 aacgaaggca atgggtctgt ggctcgtaat ggtgtgggaa cttcgtggtt gagcggtcgg
    10201 cctgtcgagg aatcaactgt cggccgtgga ttgaaaacga cattgaaccg ttcgcttcaa
    10261 tgccttcgct cctccggtct cgccccttga tcgtaaaata gcattgacct ctctgttgct
    10321 gaattcgata tgaggattga ataatgaaga tacgcattga tggcggcgtc gtcgtgggtt
    10381 ggtccggtac gggccatgaa atcatcccgg acggctcggt cgtgatagaa aacgatacga
    10441 ttcgctcggt ggggacggat aagtcgccag ccgtcgataa ggtgatcgac gcctccggaa
    10501 aaattgtttc gccgggattc gtcaaccttc atgttcattc gcagttaaac gtcggcgatt
    10561 atttgctccc cgatgtaacc aggaaggatt acctgacggc caactggttt gtttttggcg
    10621 ctcccttgaa ggaaaaagtt actccgccgc cggcgccggc agtggcaatc ggccgaaagt
    10681 atgctttatt tagcgctttg aagaatggcg caaccaccgt gctcgatccc ggcggcgggc
    10741 ccggtgcttt ggatgaatac gtcgctatcg tcggccagca gggaggacgg gtttttttca
    10801 gcccgccgta ccgtagcagc gatatcttta ccgatgcgca gggccgtctc tactatgagg
    10861 agcgtgaaga caaggggcgt cccggtctcg aaagagccgt tgcttttatc aagcaatacc
    10921 acggcgccca taacggcagg attcaaggaa tattaaatcc cgcgcaagcc gaaacgtgcg
    10981 agcccgcgct gctcaaggaa actgccgccg ccgctaaaga gctcaacgtc ccgattcacc
    11041 ttcatgccgg cggcaacgcg cgcgagtttc tgcacattct gaatacttac cgaaagccgg
    11101 tggtcgaata tcttgccggg acaggaattc tcgggccgcg aacgaccctc ggccacatgg
    11161 caatcaccgg cggccattcg ctaatcgatt acccgcgcgg cgaagaattg aagctaatcg
    11221 ccgagtccgg cgcaaccgtc ggccattgcc cgcacaaatg cgccaaaatg gcctttgcta
    11281 tggaatcatt cgaccagtac ctgaccgccg gcgtcaggat cggtcttggc accgatacct
    11341 acccgctcga tatcatcgcc gagatgcgtt acgcgtcgtt gatttctcgc ctggtcgatc
    11401 gcaacgcctc cggcgcgcga ccggccgatt gtttcaacgc agcgaccatt ggcggcgccg
    11461 atgctctggg ccggaaagat ctgggccggc ttgcagccgg cgccaaagcc gacatggtca
    11521 ttattaacat gcgcaccacc cgttacggcc catggcgtga tccgatcaac gccttagtcg
    11581 agtacggcag cggcgccgat gttgaaacgg tgattgtcga cggccaaatc gtcatcgaca
    11641 acggccggtc gacgcgcatc gacgaagatg agctatttgc tcaagcaaac gcaggtgcgc
    11701 agcgcgcgtg ggacggttgg gcaaatcgtg actggaatgg caggagcgcg gaggacatta
    11761 ttccgcctgc ttttccgacg agaagcaagt gatcatgtcg tcgggggtga cggtgtggca
    11821 aactttcatc gccatcttcc ggcactccag tttactgtag ctattcattc tggttgaaag
    11881 tcaaagaccg acaacaagag cggtgattca ttgggatgat taatcttttt tgatctgttg
    11941 cgctaggtaa ttctgagtgc cgatctcgtt gatctggtgc agctgggcct cgatccaatc
    12001 cacatgcttc tcctcgtcca ccaaaatgtg ttcgagcagt tctcgtgatg cgtcgtctgc
    12061 ggcctccagg caggtgcgta ttcctggacg gagtatcgtg agggcagcca gctcgaggcc
    12121 catgtcattt tccagctgct gcttcacgtt cgaaccgacc gagatcttgt cgtagccggt
    12181 catattggga atcgcttcga gaaagataag ccgctcgata acttcatcgg cgtgtttcat
    12241 ctcgccgatg gactcatccc acatggtttt gtagagcctc tcgtaacccc agtttttgca
    12301 catcttggca tgaatgaaat attgattgat ccccgtgagc tccttgcgca gcacctgatt
    12361 gaggacactc actacttttt cgtcgccttt catagccgcc tgcctttctc acaacgattt
    12421 ttcctgatcg tgtggcctcg tttaaaagaa ctcaacgacc cgttccgccg acggtgaccg
    12481 tcatcgtggt ggcgtcgttg ttgccgccga ccatgatcat cacggttctg gcatctttgg
    12541 cgtaagtcag cattccaccc ttggatatga cgccgacgtt gggctgccaa ccgcgtgccg
    12601 gcatctcctt tttataaaat tcaatcacgg ttcgcggttc cagttgaccg cgaaatgaac
    12661 tttgaccgcc gccgaacccg agcagtgcca cctcaatccc cgctccatcg gattttttca
    12721 tcccgcgcgg gatcggtatc tccacaccgg cgattttttc gtcataggat ctagtgcagc
    12781 cggcaaaaat caaaaccatt gccgacaaga aaagtatccg actgctagct gtcatagcgg
    12841 catgcggcga tttaaaagcc acttcgcatg ttccagtatt tataccaatg acaccgtccg
    12901 ggcaaagcag aatcaatcga cgatctaagt caagaagcag tttccggcgc cgtgggtagc
    12961 ccggtcaaat accacagcac caacagcgtc acaccgagaa attcgggcag cgcgggagag
    13021 atatggatca gtaccccgcc gagcatgccg ccaagcgatg agccgatgaa gccaaaaaaa
    13081 gtataaacac cagtggcggt gccgcggccg ccctgcggaa tcctcttggt taaaaatgtc
    13141 ggcaacaggg gttggaacaa gctgtaaccg aagaagaaag ccgcgccgct gacatacaac
    13201 agcaaatccg actgagcgcc gaccagatac acggcatacc caattgcggc ggttagccag
    13261 ccgatcataa tgggcacgcg aaagcggccc agtctttcag cgcgacgcat ataaggaaat
    13321 accaataacg cgctgggtaa aaagataatg acatagactt tccacagctc ggcacggttc
    13381 aatccgattt cctgccagct cagcggataa gtgaaaaaga aaagattgag ggtgaaagaa
    13441 agaacaaagg cggcgatgaa aatcggccgc agctcgccgt gcatcagaat cggtctaaag
    13501 gccatcgccg attctttgtc gatagatcga ttttcttttg gaaacgaaat tgccgcaaga
    13561 atcattgccg caaaagcgag cccggccaaa acatagaaca agccgctgag accgatggcc
    13621 gccgccagca gcggtccgcc caatatgccg atcataaagg cggagccaat ggcgattcca
    13681 gtaatggtaa atgcctgcgt gcgaaccgca ggccttgtaa gatcagccag cgccgccaga
    13741 gctaccgaac cgatcgcgcc ggtgccttga atagcccgcg cgatgatgag ttgggaaatg
    13801 ctctgcgcca aaccacaacc gatgctgccc gctccaaaca gcgccaggcc gatgagcaat
    13861 accggcttgc gtccccagcg atcactggcc cagcccagcg gaatttgcag aatactctga
    13921 gttagggcat aggctccgaa agcaatgccg gccatggcaa ggctggcttc ggggtaatgg
    13981 acggcataga cgctgaatac cgggaggatc agaaaaatac cgagtaaacg caatgccgat
    14041 atcagactga cgacaatcac cgggccgatt tcagcgcggc taaaggagtg gtaattgtca
    14101 gccaatgtga tgcgcgagct ccaagatata ggtccggaaa aataatcagt ccaatttaca
    14161 cgctttgccg aaaatgggaa ggctcggcga acattaccgt tgcgtggaat atgcccgggt
    14221 caattcccct ccgatggttt gatgccgagc ggcaacggcg ccttggcttc ccccacaaga
    14281 accgttctct gcggaaatgg aaactcgatg ttttcggcgt cgaatgcctt tttcaaccgg
    14341 cggcggtact ctcgccccac catccactgt tttatcggca atgtttttag ccgcgccttg
    14401 atggtgactg ctgaatccgt gaacgcattc acgccgatga tctcgatggg cgcgagcatc
    14461 gccgaaccgt attgcggatc ggattgcaac tcgccggcga cccgccgcat cacttcgacg
    14521 acgcgatcgg tatcttccct gtacgagacc ccgacgtcga tgacgtacgc cgaccagtcc
    14581 ttggtcatgt tggacagcgt attgatcgag ccgtttggaa atacgtgaac cacagcggct
    14641 tggtcgcgca ggacaatggt gcggaaggtg atcgcttcga ccaggccgcc ggttccgttg
    14701 atgaccgcga catcgccgac gcgaatttga ttttccaaaa tcagaaagaa tccgctgacg
    14761 aaatccttca ctaaattttg cgccccgaat cccaccgcga gtccgacgac gccggcgctg
    14821 gcaagaatcg gcgtgacgtt cacgccgact tgcccgagga ttgtcaaaac cgcagtgaac
    14881 cagacaagac cggcgacgat cgtccagagc acgctcatca aggttcggat tcgcatcgcc
    14941 gccgcccccg ggaccgattc cgaagattcg cgtgcccgca caagaatggc ttccagccgg
    15001 ctaagcccaa tgcggataaa cttgatggcg agcaaagccg ctatgactat taaaacgaca
    15061 cgtaatgagg actgcgcaaa attgatgctg aagtcgataa gctttggcac ataggcttcg
    15121 tacgaggttc caaacattct ctatcctcca cgagacgggt cgtttagacc cttcccgatt
    15181 ttaagtattg tacggctgcg acagcaaggc ttgtacttgc ggcagccagg gatcgttttg
    15241 aatgagcttc acgaagtttg tatggaccag tcgtgacatc gattccggcg tagagctttt
    15301 aatccgagcg agctcatcga ttactttttt gatgtccgac ggcatgccca ctatgccttt
    15361 cagccatttc aaaccgcccg gattgtctgt ttcggtgaga agcaagcgat cgggaatcgc
    15421 gcgcgcgatt gcctgaatcg tctccgaata gaggacctcg accccgacgg taaagtaagc
    15481 cccgaactgt actagggcgc cgaggatatc cagagggccc gagtaccaat gaatgatcgg
    15541 ccgcctgacg tcgtaccgct ccaacagttc gagaatggat cgctcgccgc ctttggtgtg
    15601 aaggttgacg actttttttt gctcgcgggc ggcggcaaga aaatattcca gg
//
LOCUS       DASUVS010000215        14819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1836688, whole genome shotgun sequence.
ACCESSION   DASUVS010000215 DASUVS010000000
VERSION     DASUVS010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14819
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836688"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2117)
                     /gene="ligD"
                     /locus_tag="VF182_12155"
     CDS             complement(<1..2117)
                     /gene="ligD"
                     /locus_tag="VF182_12155"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX6437879.1"
                     /translation="MASLSVKIGTKKLSLSNIDKILYPESGFTKGQVVDFYSRVAPYI
                     LPHIANRPITLKRYPNGIKGEHFYEKNAPSFTPAWIKTYPIQRSSRESMINYILINDL
                     ASLVWSANLANLEIHPFLAKAPEITVPTMVVFDLDPGDGASLVQSCEVAFLLRDLLNQ
                     LALESLLKLSGSKGIHVHVPLNTPVTYEMTQAFAQSVAQYLTREHPKLIVSEMAKIKR
                     SGKVLIDWSQNSQHKSTVAVYSLRAKAETPFVAMPVSWSELETVVNKNNAANLFFEPE
                     AALKRLEKTGDLFAPSLRLKQTLPKPFSQLSSETARAGGDARFAALEPYRRKRDFTKT
                     REPAPSVLTSRPRGGPRLFVIQKHAASHLHYDFRLEMGGTLKSWAVPKGPPYDLHERR
                     LAMATEDHPMDYANFEGIIPAGEYGGGTVMVWDIGTYELVDGNYWKGKLHILLNGKKL
                     RGEWVLVKGNDRDGKGNVWYLIKAGASMKRISEKRDNSSALTDRNLNQIAKAEDAVWH
                     SNRTAAQKLSIGNGNATSNPDLPDIRIKFIEPMLSETVEELPQNSDLWSYEIKLDGYR
                     CLALRDDSGVKLYSRKKNLLNSRFPTIVKALDSMEAGIILDGEIVALDDNGRPSFNLL
                     QNSRFTAKQIHFYVFDLLVERNKNIMGLALEKRRELLAIRLEEVADPIRLSQSIDGSP
                     EDIVAAAKELSLEGIIAKRKDSV"
     gene            complement(2213..2965)
                     /locus_tag="VF182_12160"
     CDS             complement(2213..2965)
                     /locus_tag="VF182_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437880.1"
                     /translation="MATKKSEKKTKQKAGSPKPREQEKHPDDLNPERMEGQNIGPSSK
                     RNHLGGARTAADIDVLAGRLDEFTRAELAQIPIVPVGTRLEQGAVYLDMRIPAAVPFA
                     ATADIKVTEMNYYVPKAEISYQLWDRLVAALGPATMGEASKRDEENKPFTPARAAKES
                     AAEETRGGSANRERNSEPEIDDALADSFPASDPPSWTTGRETRNAPEDETTVDDLEQL
                     TVQQLSDKARELNIEASALNREQLIRAIRSQS"
     gene            3223..3582
                     /locus_tag="VF182_12165"
     CDS             3223..3582
                     /locus_tag="VF182_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6437881.1"
                     /translation="MILSRNVDVKSLVVKSIEGKQMNGDLLVKPLIKGDEMTLLEIHY
                     TPGVGAPLHVHEHESLAYVVKGKVKMTVGKETYILGPGDVCRHPQGVPHGVEGIDESI
                     VVEIKSPAPEISSFLGM"
     gene            3617..3979
                     /locus_tag="VF182_12170"
     CDS             3617..3979
                     /locus_tag="VF182_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437882.1"
                     /translation="MPEKAKYLMIASMDVDPEHDAIFNEVYDKEHIPNLGKVPGVLGI
                     TRYRRGELTMNIGGERKTIDIPNEPTYTAIYELASPDVLTSAAWDKAIEDGRWPSQVR
                     PYTKNRRHVLLKLMEAEK"
     gene            complement(4058..4540)
                     /locus_tag="VF182_12175"
     CDS             complement(4058..4540)
                     /locus_tag="VF182_12175"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX6437883.1"
                     /translation="MPKRHPGLVRLSHDHHHGLALALRCRKQALGQIKPLGVEGLRLR
                     ASEFRTLYAAELSSHFEAEERILFPQMSLHVPESEPLIGELVRDHETVRRGVAKLQAL
                     TGLGKMIFDLGDLLERHIRREERELFPLFEQHAAVIAADEIGERLANFLELARRSNSA
                     "
     gene            complement(4605..5780)
                     /locus_tag="VF182_12180"
     CDS             complement(4605..5780)
                     /locus_tag="VF182_12180"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6437884.1"
                     /translation="MSGALAGIKIIEIASYVTGPFASQLLADMGADVVKIEEPKRGDP
                     FRGWGEKNYSATFCSLNRNKRSLTLDLRHEQAREIVLRLAAQSDVLIQNFRPGVMEKR
                     GLGYEDVRKVNSKLVYCSISGFGPKGPYRDMPGYDTIGQARSGLLSLLTDPGKPQGMG
                     ISFSDHLTGMYACYGVLAALMNRMLTGQGQLVETSLLRASISFIAENAARYFETAHVP
                     RRKHRTTTAGVFAFVDQQNLPFILHMSSPDKFWNALFKVVDRPEWSNDTRFNNRQGRI
                     ANYDILSESLQEIFKRDRREKWLKQLIDNDVPAAPLNTLDEVFDDPQVREYGFPQVMS
                     HPKMGDVKMVGNAVDMSRTPPTLNRPPPTLGEHAEDILTSLGYDIAAIKDLRSKGTV"
     gene            complement(5873..8788)
                     /locus_tag="VF182_12185"
     CDS             complement(5873..8788)
                     /locus_tag="VF182_12185"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6437885.1"
                     /translation="MTHRRKIFVRAYVGGIRRRLMLFGLVLLGAVLVLSTFAGSSYTR
                     RQIYESTAALQTEIASATARQIHHFMTRKVERLRDAGVFMSRYPIGSEEQRLMTLLLL
                     KNDPAFRQISILDKEGMERVKSSEILAFQSADFQNRSGETNYTQAIQGKIYIGPVRTS
                     DRAEPYIVIAMPLMESPRKAIGVMVAQVHLRFLWEVIAGSRFRRGGYAYLIDENGEII
                     AHEDPSLVLKRLNLGAVAKVQRFLAHRASDPAPAELGAGINGAQVLSTYAEVPDLRWA
                     VIVEQPAAQALSDLERLHRYSILLIGSGLLVGSGIIVWVSRKISRPIQELRQAVRTIR
                     GGNLEHRAEITTGDEVEDLAQEFNDMAEALQTSYATLEQKVRQRTEETAALYEVTTAV
                     NKSLDLQIILQAVIDKITGIFQFETTRIFLFNAEMEQMELRASFEVDPEHWTEVHTFR
                     RGQGVIGRVVESGEPMIFENIETDPIYASLSSSRATQKAKLRFFGVLPIKTQSCAFGA
                     IVFNGKSPRKLSADQIRLLTSMAEHLGVAVEKINLFDEVNTRSRHLAVLHTIGAAVSR
                     SLNLETVLKEAVRKIAETLNFDAVWIYELEAEKGILHLRGQTGLSDEIVKGMAERSVA
                     SGISGQVVRTGRPLVFEDIVNDPQYRELTSAGRIVSLGFQAAAAFPIVVKESIAGTLH
                     VVSRAKRHFSQDEQQLIQSIAQEIGVAVENARLFAEVKKTTSELEKTNQELLDATRAK
                     SEFISAMSHELRTPLHVIIGNADLAQDEFFGPLNADQKTALQKILRNSRVLLKMINDV
                     LSLSRIEARKMSLDLATVEIEELIEHARHQVEQINRDGRLEVNWDIDENLPPVTTDPI
                     KLEEILQNLIANACKFTPKGQVDVRVRTLMDRDRVQFSVADTGIGIEAEDVRRIFQEF
                     EQIKEAHTGQFDGVGLGLSIVKKYLDLLDGEIRVESQPGQGSIFTFSIPRSLRRHAA"
     gene            complement(8794..9822)
                     /locus_tag="VF182_12190"
     CDS             complement(8794..9822)
                     /locus_tag="VF182_12190"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437886.1"
                     /translation="MGPGTAIPLTDPVRANRFPIATLIRLSVMVIALIIIPVSPSEAQ
                     KVYSIGSLNTGDSFINAFEGFRARMTELGYRERQNVQYQYYNSKGNEELLRTIAHKLV
                     HEKVDLIVTSSTTATVAAARATQGTPIPVVFLSAGNSQNLFKSFSGSGSNLAGISSAS
                     LELTGKRFELLKELAPGTKKIAIPLDPNGVNYKAIVAEVQATAPKFGLGYTEIHVRQV
                     EEIGTMVQSITRKKYDAIYHPPDSLVTEGVQQVVEQAIKEKLPLVTSLLVNVKRGCLA
                     TYAADYPALGKQAAALADKIFKGIKPSELPIELPYKLNLVLNLQTAKAIDLKLAKEML
                     LRADQVFE"
     gene            complement(10201..10917)
                     /locus_tag="VF182_12195"
     CDS             complement(10201..10917)
                     /locus_tag="VF182_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6437887.1"
                     /translation="MKPLLRIAGLHVHYGAIEAVKGIDLTLGQGRITTLLGANGAGKS
                     TILLTISGVVKASSGSIHFDGMEVQNLSPHRIVGHGIVQVPEGREILTTLTIKENLLL
                     GAYRQSRHKANLATDLENVLSLFPRLKERLDGTAGNLSGGEQQMLAIARALMARPRLL
                     LLDEPSMGLAPLLVQEIFKTLRELNDRGLTIFLVEQNARQALKIADDAYVLENGKTVL
                     SGAGAEMLNDPRIIQAYLGG"
     gene            complement(10914..11702)
                     /locus_tag="VF182_12200"
     CDS             complement(10914..11702)
                     /locus_tag="VF182_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6437888.1"
                     /translation="MLSLSGLSKNFGGLQVLQNVTLTIPERAIVGLIGPNGAGKTTVF
                     NLITGLLVPAKGTIELFGRLLNGMPPYQITRCGVARTFQNIRIFKEMSVLENVLVALR
                     PSHDYNPGRAFLSLRGLHDSRPREEEAALELLRRVSLIDMVAQPAGTLSYGAQRRLEI
                     ARALATSPKLLLLDEPAAGMNPVEKQQLMDEIVNLHQSGLGVLLIEHDMSFVMGLCKQ
                     IIVMNFGQVIAAGSPEEIRQNPRVIEAYLGRDDETNPTVSQPRS"
     gene            complement(11706..12566)
                     /locus_tag="VF182_12205"
     CDS             complement(11706..12566)
                     /locus_tag="VF182_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6437889.1"
                     /translation="MDWFDNFWATYNTLVLSVGTNVLLALSIYLTLACGLLTVANAAF
                     MAIGSYSSALLTLHTGTPFFVALFVGSLLPALVALVIGRPTLKLSGVYLAMATLAFGE
                     VVRIALLNSEDLTGGALGLNGIPQLTGWFSVALAVSVVVYILLRSARSRFGRTMAAIR
                     QDEVATEVIGINVRAYKLFAFVVGAALAGLAGGLNAHFTFFISPNEYGFDRGVEILAM
                     GVLGGTGSPWGAVLGGVLITLLPELLRGLGDYRPLINGIILILIILYSPRGLWGLIKL
                     ARRSPNYGGN"
     gene            complement(12620..13501)
                     /locus_tag="VF182_12210"
     CDS             complement(12620..13501)
                     /locus_tag="VF182_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6437890.1"
                     /translation="MFAQQFINAVSLGGVYALFALGFTLVFGVLGVVNLAHGAVFMLG
                     AYSALQAVVILQLPLPAAVLTAMLITAATGWLLDKLIFAPLRARQAPHLAPMIATIGL
                     AIALNSSIQGFFGAQNLRFPVEALPVQTLSIGGAQITWLEIEIVVLAFILMAVLLWGM
                     RRTRIGCALRGLAESPKAAAFLGINVEGLFRLTSVIAALLGGAAGVLIALYTNAVFPT
                     MGQPMLHKGIAVVILGGMGDMRGALLGGFFLGFAEVFSVAYIGSSMRDAVGFGLLFAI
                     LLLRPQGLFGTAAQRRA"
     gene            complement(13508..14653)
                     /locus_tag="VF182_12215"
     CDS             complement(13508..14653)
                     /locus_tag="VF182_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017685419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437891.1"
                     /translation="MKPAILMAMATVFLGSPIVGAAEIKIGVAAALTGNAAQYGVPIR
                     KGFELAVREINDSGGIGGTGLELVIEDEQGKKEEAINSFRKLIFQDKVLMLFGPTLSN
                     SAQASDPVAQAARTVAFGTSNTADGITSIGDYVFRNSVTERDILPVTLKVAAQKTGMK
                     KVAVLYGNDDIFTKSGYDNFKKALQDLKIPVTATETFAKGDVGFKPQLTNIKASSPDA
                     IVLSALVAEGAPIMVQARQLGIQLPFIGGNGMNSPRIFELAKNSSENLWVGSPWSVES
                     PTSENKRFIASYQKAYLEMPDQFAAQAYDGMYIAAQALKKIKLTGKVEADRKALRDAL
                     PATQWNGATGPFKFRQVNGRDGKPAGYDAVQTAIVMMTTGNRYVIQK"
BASE COUNT         3482 a         4054 c         3776 g         3507 t
ORIGIN      
        1 tacaccgagt ctttcctttt ggcgataatg ccctcgagcg acaactcctt tgccgctgcg
       61 acgatatcct ccggtgagcc gtcaatgctt tgcgacaatc ttatcggatc ggcaacttcc
      121 tccaagcgga tggccagcaa ctcacgcctt ttctcgagcg cgagccccat gatgttttta
      181 tttctctcaa ccaaaaggtc gaacacatag aaatgaatct gcttggccgt gaagcgagag
      241 ttctgcagaa gattgaatga cggcctgccg ttatcatcaa gtgcgacgat ttcgccgtcc
      301 aaaataattc ccgcttccat gctgtccaag gctttaacga tggtcggaaa acgcgagttg
      361 agcaaattct tctttcgcga gtacagcttc acgccgctgt catctcgtag cgccaggcag
      421 cgatagccgt cgagctttat ttcatagctc cacagatcgg aattctgcgg caactcctcc
      481 acggtttcag acagcatcgg ctctatgaat ttgatccgga tgtcgggtaa gtcgggattc
      541 gaagtcgcgt tgccgttgcc tatcgagagt ttctgcgccg ctgttctgtt gctgtgccag
      601 acggcgtcct ccgctttggc gatctggtta agatttcggt ctgtcaatgc tgaagaattg
      661 tctctctttt cggagattcg cttcatgctc gcgccggctt ttatgaggta ccaaacattg
      721 cctttaccat cgcgatcatt ccctttcacg agcacccatt cgcctcggag ctttttgccg
      781 ttcagcagga tatgaagttt tcctttccaa taatttccat cgacaagctc gtaagttcca
      841 atgtcccaga ccatcacggt accgccaccg tattcacctg ccggaataat cccctcaaaa
      901 ttggcgtagt ccatcgggtg atcttccgtc gccattgcca accttcgctc gtgtagatca
      961 tagggcggcc ctttgggtac tgcccatgat ttcaaagtgc cacccatttc cagtcggaaa
     1021 tcataatgaa ggtgactggc ggcatgcttc tgtatgacaa acagccgggg tcctcctcga
     1081 gggcggctcg taagaacaga aggcgccggt tcccgagtct tagtgaagtc gcgtttgcga
     1141 cgatagggtt caagagcagc aaagcgtgcg tcgcccccgg cacgcgcagt ttcggaactg
     1201 agctgcgaga acggctttgg caaggtttgt ttcaggcgca atgacggcgc aaataaatcg
     1261 ccggttttct ccaacctctt caatgccgcc tctggttcaa aaaatagatt ggcagcgtta
     1321 ttcttgttca ccacagtttc caattcgctc caacttaccg gcattgcgac aaacggcgtt
     1381 tccgcctttg ctcttaacga ataaaccgcg acggtcgact tatgctgtga gttttggctc
     1441 cagtcgatga gtacttttcc gctgcgtttg attttagcca tctcggatac gatcaattta
     1501 ggatgctcac gggtaagata ttgggcgacg ctttgggcga acgcttgagt catctcgtag
     1561 gtcacgggtg tattcagagg cacgtgcacg tgaatacctt tagaccccga cagcttaagc
     1621 aaggactcga gcgccagttg gttgagcaga tctcgtagta gaaatgccac ctcacaggat
     1681 tgtacgaggc ttgctccgtc tccgggatcg agatcgaaaa cgaccattgt cggaacggtg
     1741 atctcaggtg ccttagcgag aaaggggtga atctcgagat tcgctaaatt tgccgaccat
     1801 actaaactgg ccaggtcgtt gatcaggatg taattgatca tcgattcgcg ggagctgcgt
     1861 tggatcgggt aggtctttat ccaagcgggc gtgaaagagg gagcattttt ttcatagaaa
     1921 tgctcgccct tgatgccatt tggatagcgc ttgagtgtga tcggccgatt tgcgatatgg
     1981 ggcaatatgt aaggggcgac ccggctgtag aaatcaacca cctgcccctt ggtaaagccg
     2041 ctttcgggat aaagtatttt gtcaatgtta gagagagaga gtttcttggt gccgatcttg
     2101 acggacagcg aagccatcaa cgaattctat cgcgccaagg ggaagatgca atgaactttg
     2161 agcgcgcata gactcgcgcc tggttgagcg cgagccctgc acgcatgaaa cgctacgatt
     2221 ggcttcgaat tgcccggatc agttgctccc ggttcaacgc ggaagcttcg atgttcaact
     2281 cacgcgcttt atcactgagc tgttggacgg taagctgctc gagatcatca acagtcgttt
     2341 catcctccgg cgcgtttctg gtctcgcgcc ccgtggtcca tgatggcggg tcgctcgcgg
     2401 gaaaagagtc ggcgagggca tcgtcgatct ccggctccga atttctctct cggttcgcgc
     2461 tcccgccgcg agtttcctct gcagccgatt cctttgccgc cctggcgggc gtaaaaggtt
     2521 tgttttcctc gtctcgttta gacgcttctc ccatcgtcgc cggccccaaa gctgcgacca
     2581 acctatccca tagttgatac gatatctccg ccttcggcac atagtaattc atctctgtaa
     2641 ccttgatgtc ggcggttgcg gcaaatggca ccgcggccgg aatccgcata tctaggtaaa
     2701 ctgcgccttg ctctaatctt gtcccgacgg gaacgatcgg aatttgcgcc aactcggcgc
     2761 gcgtaaactc atccaagcgg ccggcaagaa cgtcgatatc ggcggcagtg cgtgcgcctc
     2821 ccaaatggtt gcgttttgaa gaaggtccaa tattctgtcc ttccatccgc tcaggattca
     2881 aatcgtctgg atgcttttcc tgttctcgcg gtttaggtga tccggctttc tgtttggttt
     2941 ttttctcgct cttttttgtt gccattaatg ctcctgcgga agtcaatttt gtttgtgcta
     3001 agcacgcgaa aacgcaaccg atgtgccacc ccgttagcgt gccaaattgg ggatacacga
     3061 gatctgtgtt ccatttctgc gaaagaaatc cgatcctgtg atccattttc aaaactagcc
     3121 tattccttga ccggagccag gatacaaacg atagactcgc cgcgcattta ctgatagtta
     3181 gcattcagcg gacctttttt gctgcagaac ggagggagtg agatgatttt atcaagaaac
     3241 gtggacgtga aatcgctcgt cgtcaagtcg atcgagggca agcaaatgaa tggtgatcta
     3301 ttagtcaaac cgcttatcaa aggcgacgaa atgacactcc tggaaattca ctatacaccg
     3361 ggcgtgggag cgcctttaca cgttcacgaa cacgagtctc tggcctatgt tgtcaagggc
     3421 aaggttaaga tgacggttgg caaagaaacc tacattctcg ggccgggcga tgtttgccgg
     3481 catcctcaag gcgtgcccca cggtgtcgaa ggaatcgacg aatccattgt cgttgaaatc
     3541 aaatccccgg ctcctgaaat ctctagcttc ttgggaatgt aaatcgagcc aacagcacgg
     3601 acgcaggaga acagctatgc cggaaaaagc aaaatatcta atgatcgcga gcatggacgt
     3661 cgatccggaa cacgatgcga tattcaatga agtttacgac aaagagcata ttccaaattt
     3721 aggcaaggtg cccggggttc tgggcatcac tcggtaccgc cgaggtgagc tgacgatgaa
     3781 tatcggcggt gagcgcaaga ccatcgatat tcccaatgaa ccgacctata ccgccatcta
     3841 tgaattggcg agccccgacg ttctaaccag cgccgcgtgg gacaaagcaa ttgaagatgg
     3901 ccgctggccg agtcaggtga gaccttacac gaagaatcgg cgtcacgtgc tcctgaagct
     3961 gatggaggcg gagaagtaat cgcttcgcat catgctctgc agcagtgctt ttgcggctta
     4021 tatgaagtga agtcaatgac gcgcagggtg acgatgttta cgccgaattg cttcttctag
     4081 caagctcgag aaaattggct aatcgttcgc cgatttcgtc agcggcaatg acggcagcat
     4141 gctgctcgaa caagggaaag agctcgcgtt cttctctgcg aatgtgacgc tctaaaagat
     4201 caccgagatc aaaaatcatt tttccgagac ctgtaagcgc ctgcaacttc gcgacacccc
     4261 gtctcacggt ttcgtgatcg cgcactagct cgccgattag cggctcgctc tcaggcacgt
     4321 gcaagctcat ttgcgggaaa agaatccttt cttcagcctc aaagtgtgag ctaagctcag
     4381 cggcgtaaag agtacgaaac tcactcgcgc gcaaacgtaa gccttctacg ccgagtggtt
     4441 tgatctgtcc caacgcttgc ttgcgacaac gtaaggcgag cgccaaacca tggtggtggt
     4501 cgtgggaaag acgaaccaat ccggggtgtc gcttcggcat actcgaccat tatcggcagt
     4561 gttttaacga aagaaaatgg cagcctgatg cggcaaccca atcgtcagac ggttcccttg
     4621 gacctcagat ccttgatcgc cgctatgtca tagcccagcg aggttaatat atcctcggca
     4681 tgctctccta acgtgggtgg cggtcgattc agggtcggcg gcgtgcggct catatcaacc
     4741 gcattaccaa ccatcttaac atcgcccatt ttgggatgac tcatgacttg aggaaagccg
     4801 tattcccgga cttgcggatc atcaaacact tcgtccaagg tattgagcgg cgccgcgggc
     4861 acatcgttgt caatcaattg cttcaaccat ttttccctcc gatcgcgttt gaaaatttct
     4921 tgcagagatt cagagagaat gtcgtaattg gcgatgcgcc cctgccggtt gttaaaacgc
     4981 gtgtcattgc tccactccgg tctgtcgaca accttgaaca gggcattcca gaacttatcc
     5041 ggcgacgaca tgtgcaggat gaacggcaga ttttgttgat ccacgaaggc aaagacccct
     5101 gcggttgtgg ttctatgctt gcgccggggc acgtgcgccg tttcgaaata gcgcgccgcg
     5161 ttttcggcaa tgaacgagat ggatgctctg agcaatgatg tttcgacgag ttgcccctgt
     5221 cctgtcagca tccggttcat cagagctgcc agaacgccgt agcaggcata cattccggtc
     5281 aaatgatccg agaaagaaat ccccatccct tgaggcttac ccggatcagt aagcaaactg
     5341 agcagaccgc ttcgcgcctg gccgatggtg tcgtacccgg gcatgtcccg gtaaggtccc
     5401 ttgggaccga atccggaaat tgagcagtag acaagcttgg aattgacctt acgaacgtct
     5461 tcataaccga gcccgcgttt ttccattacc ccgggtcgaa aattttggat caaaacatcg
     5521 ctttgcgcgg ctagcctcag aacgatctcc cgagcttgct catggcgcag atccaaagtt
     5581 aagcttcttt tgtttctgtt gagactacaa aaggtggcgg aatagttttt ttcaccccat
     5641 ccacggaatg gatcaccacg cttaggctcc tcaatcttga cgacgtccgc gcccatatcg
     5701 gccaacagtt gcgaagcgaa cggtcccgtc acataactag caatttcgat gatcttgatt
     5761 ccagctaaag caccgctcat ctatcacctc tccgccgcta acccaaagtg gctggcgatt
     5821 ggatattacc ttcctaactt tgggcggcgt aaaaagtcaa gcgcgagcgc gctcaggccg
     5881 catggcgccg cagcgatcga ggaatcgaaa aagtaaaaat ggacccttgg ccgggttggc
     5941 tttcaacccg gatctcacca tcgagtaagt ctaggtactt tttgacgatg ctgagaccga
     6001 gacctacgcc atcgaattgc ccggtgtgag cctctttgat ttgttcgaac tcctggaaga
     6061 tccggcgaac atcttcggcc tcaatgccga tgccggtgtc ggctacgcta aattgaacgc
     6121 ggtctctatc cataagggtg cgcactcgga cgtcgacttg gccttttggg gtgaacttgc
     6181 acgcattagc aatcaagttt tgcagaatct cttcgagctt gattggatcg gtagtgactg
     6241 gggggaggtt ttcatcgata tcccagttca cctctaggcg gccgtctcga ttgatttgct
     6301 cgacctggtg ccgggcatgc tcgatgagtt cttcaatctc cacggtggca agatctagcg
     6361 acatttttcg tgcttcaatg cgcgacaggc tcagcacatc attgatcatc tttagcagaa
     6421 cacgggagtt ccgcagaatt ttttgaagcg cggttttctg atcagcgttg agcggaccaa
     6481 agaattcgtc ctgtgccaga tcggcattgc caatgatgac atggagcggc gttcgcagct
     6541 catgtgacat ggccgaaata aactctgatt tggctcgtgt cgcatcgaga agctcctgat
     6601 tggttttctc gagctcggaa gtcgttttct tgacttcggc aaacagtctg gcgttttcca
     6661 ccgcgacgcc gatttcctgc gcaatggatt ggatcagctg ctgctcatct tgagaaaagt
     6721 ggcgcttggc gcgactgact acatgtaatg ttccggcaat actctctttg acgacgatcg
     6781 gaaacgcggc agcggcttga aatcccagcg agacaatcct ccccgcgctg gtcaactcgc
     6841 gatattgggg atcgttgaca atatcctcga agacaagcgg gcggccggtt ctcacaactt
     6901 gaccgctaat tccactcgct acactgcgct cggccatgcc tttgacgatt tcatcgctga
     6961 gcccggtttg cccccgtaga tgcaaaattc ccttctccgc ttccagttcg tagatccaga
     7021 ctgcgtcgaa attaagtgtc tctgcaatct ttctaacagc ttccttaaga acggtttcca
     7081 gattgagcga acggctgacg gcggcaccga tggtgtgtag cacggccaga tgccgcgatc
     7141 tcgtgttaac ctcgtcgaat aagttgatct tctctaccgc gacgccgaga tgctccgcca
     7201 tggaagtcag cagacgaatc tgatcggcgc tcagctttct aggcgatttg ccattgaaga
     7261 caatggcgcc aaaagcgcag gactgggttt taatcggcag cacgccgaaa aaacgcagct
     7321 tggccttctg cgtggctcga ctgctgctga gagaagcata aatcggatcg gtttcgatat
     7381 tttcgaagat cattggctcg cccgactcga cgacccggcc gatcacgccc tggccccttc
     7441 tgaaagtgtg gacctcggtc cagtgctccg ggtcgacctc aaacgatgct cgcaactcca
     7501 tttgctccat ttccgcattg aaaaggaaaa ttcgggtcgt ttcaaattgg aagataccgg
     7561 tgatcttatc gataaccgcc tgcaggatta tttgtagatc cagtgatttg tttacagctg
     7621 tcgtcacttc ataaagcgcc gcagtttctt cagttcgctg tcttactttc tgttccaggg
     7681 tggcatacga ggtctgtagc gcttcggcca tgtcattgaa ctcctgcgcc aaatcttcga
     7741 cttcatcacc ggtagtgatc tcagcccgat gctcaagatt gccaccacga atggttctga
     7801 cagcctgacg gagttcctga atcggtcgac taatcttccg actcacccag acaataattc
     7861 ctgaacccac taaaagtccg ctaccgatta gcaaaataga ataacggtgc aatcgctcaa
     7921 gatcggataa agcttgagca gcgggctgtt cgacgattac cgcccatcgc aggtccggta
     7981 cttcggcgta ggtgcttaac acctgcgcgc cgttaatgcc ggcccccagt tcggctggag
     8041 cgggatcgct ggctcgatgc gcaaggaagc gttgcacttt agcaaccgct ccaagattta
     8101 gacgcttaag caccagcgaa ggatcctcgt gcgcgataat ttcgccattc tcgtcgatca
     8161 agtaggcgta gccaccacgg cgaaaccgac ttccggcgat cacttcccag agaaatcgaa
     8221 ggtgaacctg cgccaccata acaccgattg ctttacgcgg actttccatc aatggcatgg
     8281 ctataacgat gtagggctcg gcacggtccg aagtgcggac cggaccgata tagattttgc
     8341 cctgaatagc ctgggtataa ttagtttcac cactccggtt ttggaaatca gcggattgaa
     8401 aggcaagaat ctccgaggat ttgactcttt ccattccctc cttgtccagg attgaaatct
     8461 gcctgaaagc aggatcgttc ttgagcagca acaatgtcat caagcgttgt tcttcgctgc
     8521 cgatagggta acgactcata aacacacccg catcccgtaa ccgctccacc ttgcgagtca
     8581 tgaaatgatg tatctgtctt gcggttgcgg aggcgatttc tgtttgcagc gccgcagtgg
     8641 actcgtagat ttgtctgcgc gtgtagctcg acccagcaaa tgtgcttagc accaggactg
     8701 ctcccaacag aaccaagcca aaaagcatca agcggcgtct aattccgccg acatacgccc
     8761 ggacaaagat tttccgccga tgcgtcatcg ccctcactca aacacttggt cggctcttaa
     8821 caacatttcc ttggcaagtt tcaaatcgat ggcttttgcg gtctgcaggt tcagcactag
     8881 attaagcttg tacggcaact cgatgggcag ctcagacggt ttgattcctt tgaagatctt
     8941 gtccgccagc gccgcagctt gcttgccgag cgcgggataa tccgcggcat aggtagccag
     9001 acagccacgc ttgacgttga ccaacaacga agtcaccaac ggcagctttt ctttgattgc
     9061 ctgctcaacc acctgctgaa caccttccgt cactaaactg tccggcgggt gataaatggc
     9121 gtcgtacttc ttgcgagtaa ttgactgaac cattgtgccg atctcttcaa cctggcgcac
     9181 atggatttcc gtatagccaa gaccaaactt gggcgctgtc gcttggactt ccgcgacaat
     9241 ggctttgtaa ttgacgccgt taggatctaa cggtatggcg atctttttgg ttccgggggc
     9301 taactccttg agtagctcga agcgcttgcc ggtcaattcc aaagacgccg aactgatgcc
     9361 ggcaaggttg ctacccgagc cggaaaagct cttaaaaagg ttctgcgagt ttcccgcgct
     9421 caggaagaca accggaatcg gtgttccttg cgtcgctcgc gccgctgcta ccgtggcagt
     9481 agtcgaagag gtaactatca gatcgacttt ctcgtggacc aacttatgcg cgatggttcg
     9541 gagcagctct tcattgccct tcgagttata atactgatac tgaacatttt gccgttcacg
     9601 ataacccaat tcggtcatac gtgctctaaa tccctcgaac gcgttgataa acgaatcacc
     9661 ggtattgagt gaaccgatgg aataaacttt ttgcgcctcg ctcggagata ccgggatgat
     9721 tatcagagcg ataaccatca ccgacaacct aatcagcgtg gcgattggaa atctgttggc
     9781 gcgcacaggg tctgtcagag ggattgctgt tccaggtccc atcggtcctc cggatcgact
     9841 tagcactttc ttcgcgccgg aagctcttca acgtgagtcg aggaaagtct gatagatttg
     9901 ttcaagctac cgtaaccgac gaggtaagtc ggcatcttcc gaaaaacgcc ttatcaaggc
     9961 tttggctacc gacgcaggat ttgcgccaga gcaaagatca actcttgaag agcagctcta
    10021 gtgatttacg cagatttgcg cagagtcttt gtcaaattct acgcgagaga ggaaacgcgg
    10081 cgctgaaaat ttttgacgaa atcatactga atacgccata tttggctaga ttggcacgtt
    10141 atggctatgg tcggacgcag ctataatcct gcgcgcaagc cgttatgtaa ctcccgccgg
    10201 ttacccgccc agatacgctt ggatgatcct ggggtcgttc agcatctccg cacccgctcc
    10261 ggacagcaca gtcttgccgt tttccagaac gtaagcgtca tccgcgatct tgagcgcttg
    10321 ccgggcattc tgttcgacta aaaaaatcgt caagccgcgg tcgttcagtt ctcgcagagt
    10381 tttgaaaatt tcttgcacca ggagcggcgc taaacccatc gagggttcgt ccaatagcag
    10441 caaccggggt cgtgccatta gcgcacgggc gatggccaac atctgctgct cgccgcctga
    10501 gagattgcct gcagtcccat cgagtcgctc ttttaatcgc gggaacaaac ttaaaacatt
    10561 ctctagatcc gtggccagat tcgctttatg tctgctttgg cggtaggctc cgagcaacaa
    10621 gttctctttg atcgtcaagg tagtaagaat ttctcggccc tccgggacct gcacaatccc
    10681 atgcccaacg atccggtgcg gcgaaaggtt ctgcacttcc atgccatcaa aatggatcga
    10741 tccggagctc gctttcacaa ctcccgaaat cgtcaagagg atggttgatt tacctgcgcc
    10801 gttggctcct agcagtgttg ttatccgccc ttgaccgagc gttagatcaa tccctttaac
    10861 cgcttcgatg gcgccgtagt ggacatgcaa accggcaatg cgcagaagcg gtttcatgaa
    10921 cgaggttggg agacagtagg attcgtttcg tcatcccgtc ccaaatacgc ttcgataact
    10981 cggggatttt ggcgaatttc ttcggggctt ccggccgcga ttacctgacc gaaattcatc
    11041 acaatgattt gcttacagag tcccatgaca aagctcatat cgtgctcaat cagtaatacg
    11101 ccaaggccac tttgatgtag gttgacaatt tcgtccatca attgctgttt ttcgacagga
    11161 ttcatgccag ccgccggttc atccaagagc agcaacttgg gcgaagttgc cagcgcgcgc
    11221 gcaatctcta aacgccgctg tgcgccatac gaaagtgtgc ctgctggttg cgccaccatg
    11281 tcgattaggc tgactcttcg caagagctcg agcgcggcct cttcttcacg gggccttgag
    11341 tcatgtaaac cgcgtaagga gaggaacgcc cgccccggat tgtagtcgtg ggacggtctg
    11401 agcgcgacga gaacattttc cagcaccgac atttctttga agatccgaat attctgaaaa
    11461 gtacgtgcga caccacaacg ggtgatctga taaggaggca tgccgtttaa cagtcgtccg
    11521 aagagttcta tggtcccctt cgcgggcacc agtaagcccg tgatgagatt gaagacggtt
    11581 gtcttaccgg cgccgttcgg accgatcagt ccaacaatcg cccgctccgg tatcgtcaga
    11641 gtgacatttt ggagcacctg caagccgccg aaattcttcg aaagacccga caagctcagc
    11701 atctctcaat taccgccata gtttggcgac cggcgcgcaa gctttatcaa accccaaagt
    11761 ccccggggcg agtacagtat gataagaatc aagatgatcc cattgatcaa tggtctatag
    11821 tcacccagtc cacgcagcag ttccggtaaa agcgtgatca agacgccgcc gaggaccgca
    11881 ccccatggac tgccggtacc gccaaggaca cccatcgcaa gaatttccac gccacgatcg
    11941 aagccatatt cgttaggact gataaagaat gtgaaatgcg cattaagtcc acccgccaat
    12001 ccggccaacg cagcgccgac cacaaaagca aataatttat aggcgcgaac gtttatcccg
    12061 atcacttcgg tggcgacttc atcctgacgg atcgccgcca tcgtgcgacc gaagcgggac
    12121 cgtgcagatc gcaataaaat atacaccaca acggatacag ctagcgccac agaaaaccag
    12181 cctgtcaact gcggaatacc gttcagccca agggcgccgc ctgttagatc ttcgctattg
    12241 agcagcgcta tccgaaccac ttcaccgaaa gccaaagtcg ccatcgccaa ataaaccccc
    12301 gagagtttaa gcgtcggacg gccaatcaca agcgcaacca gagcgggcag caacgagccg
    12361 acaaacagcg ccacgaaaaa tggcgttccg gtgtgcaaag taagtagcgc ggagctatag
    12421 ctcccgatag ccataaaagc cgcattagct accgtcaaca agccgcaggc taaagtcaga
    12481 taaatcgaca aagccagaag cacgttagtg ccgacagaga ggactagcgt gttgtaggtt
    12541 gcccagaagt tatcgaacca atccatgctc cgttgggttg cggtgctctc agaggagagt
    12601 gatcaagcag cgaagagcgt tatgctcggc gctgagcggc agttccgaaa agaccctgag
    12661 gccgaagcag gagaatcgcg aagagcagac cgaagccgac agcatcacgc atgctcgagc
    12721 caatgtaagc gactgagaaa acctcggcaa atccaagaaa aaaccccccc aataaagcgc
    12781 cgcgcatgtc gcccatgccg ccgagaatca caaccgcgat gcctttgtgc agcatgggct
    12841 ggcccatggt agggaagacc gcgttagtgt acaaggcaat cagaacgccg gcggcgccgc
    12901 cgaggagcgc cgcaatcacc gatgtgagcc ggaacaatcc ctccacattg atccctaaga
    12961 atgccgccgc ttttggagat tcggccaagc cacgcagtgc gcaaccaatg cgggtccgcc
    13021 gcatgcccca gagcaataca gccataagaa taaaggctaa gaccactatc tcgatttcca
    13081 accaggtgat ttgcgcgccg ccgatgctta acgtctggac aggcaaggct tcaactggaa
    13141 agcgcaaatt ttgcgcgccg aaaaaacctt gaatcgagct atttagggct atagccaaac
    13201 cgatggtcgc aatcatcggc gccaggtgcg gcgcctgacg cgcccgcaag ggcgcgaaga
    13261 tcagcttgtc gagaagccaa ccggtagcgg ccgtgatcag catcgccgtc aggaccgctg
    13321 ccggcagcgg cagctgcaga atcacaaccg cctgcaaagc ggaataagct ccgagcatga
    13381 acaccgcgcc atgcgccaga ttcaccacgc ccaatacgcc gaacaccaag gtaaaaccca
    13441 gcgcgaaaag cgcataaact ccgcccaagg aaacagcatt gatgaactgc tgcgcgaaca
    13501 tggacgacta tttttgaatc acgtaccggt tgcctgtggt catcatgaca atggcggttt
    13561 ggacggcgtc gtagcccgcg ggcttgccgt cgcggccatt aacctggcga aatttaaacg
    13621 gacctgtggc cccgttccac tgcgttgccg gaagagcgtc gcgcagcgct ttgcgatccg
    13681 cctcgacctt acccgttaat tttatcttct ttaaagcctg tgccgcgata tacataccat
    13741 cgtacgcttg ggccgcaaac tggtcgggca tctccagata ggctttttga taggaggcga
    13801 taaaacgctt gttctcggac gtcggacttt ccacggacca agggctgcca acccacaggt
    13861 tttcagagct gttcttggca agctcaaata ttcgtgggga gttcatcccg ttgccgccga
    13921 tgaacggcaa ttggatgccc aattgccggg cctgtaccat gatcggggcg ccttcggcga
    13981 ccaaagcgga aagcacgata gcgtcaggac tgctggcttt gatattggtc agttgtggct
    14041 taaatccaac atcccctttt gcgaaagtct cggtcgctgt aaccgggatc tttagatcct
    14101 gcagagcttt cttgaagtta tcgtagccgc tcttagtaaa gatatcgtca ttgccgtaaa
    14161 gcactgcgac tttcttcatg ccagtcttct gtgcagcgac tttaagcgtc accggcaaga
    14221 tatcccgctc ggtaaccgaa ttacgaaaca catagtcgcc aatcgaagtg attccgtccg
    14281 cggtattcga agtaccgaaa gcaaccgttc tggcagcctg cgcaacgggg tctgaggcct
    14341 gcgccgaatt ggacagcgtc gggccaaaca gcatcagcac tttgtcctgg aagataagct
    14401 ttctaaaact attgatagcc tcctcctttt tgccctgctc atcttcaatg accaattcca
    14461 atccggtccc gccgatgcca ccgctgtcat ttatctctcg aacggctagc tcaaatcctt
    14521 tccggatcgg caccccatat tgcgccgcat tgccggtcaa ggcggcggcg actcctattt
    14581 tgatctcggc agcgccgact ataggggaac ccaaaaagac cgttgccatc gccatcagta
    14641 ttgccggttt cattttcact gaagacacct accgcaaaat attcgacaaa cgtcgatgaa
    14701 catcactcta tctcgtcatg gtcatccaaa cattagcggc tcacaacaac tagttcatag
    14761 aactggccgc tcattttgaa gtcgccaggt tcgtcgggac catactagct gatgtgatt
//
LOCUS       DASUVS010000216         4092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_744248, whole genome shotgun sequence.
ACCESSION   DASUVS010000216 DASUVS010000000
VERSION     DASUVS010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744248"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..637)
                     /locus_tag="VF182_12220"
     CDS             complement(239..637)
                     /locus_tag="VF182_12220"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6437892.1"
                     /translation="MSQDNIAVIRGLYESFSTGDVTSVLGQMHQHIEWREAENFIYAD
                     RSPYRGPQAVLEGVFMRLASEWADFKVMPEEWLDAGNHIVVLGNYSGRHKESGKEVRA
                     QFAHIWGVTHGRVVRFQQYTDTKQFADAIG"
     gene            964..1290
                     /locus_tag="VF182_12225"
     CDS             964..1290
                     /locus_tag="VF182_12225"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6437893.1"
                     /translation="MTSMIRVAVLLVVVFAVTGCQALTGRTAGRNIDDLTITASVKAR
                     LVADKLSNFTRIDVDTVNAVVSLTGLVESEFEKRRAEDIASLVEGVENIINDLQVQKE
                     PAAKSE"
     gene            complement(1559..2395)
                     /locus_tag="VF182_12230"
     CDS             complement(1559..2395)
                     /locus_tag="VF182_12230"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX6437894.1"
                     /translation="MELIERIQDRYSSLSKNHRKVADYIANNGMAASFDSITELSQKV
                     GVSEASLVRFAKELGYKGYPSLRKELQAEYKRTSGTASRVGDAVTTVGERSFPKSMFQ
                     HDIDLIKETQSSLTKASFTQAVRMIFGAKKIFIIGFRAAFALAYFLHFRLLRIGLDAR
                     LIMLTGGTSLVEQLAQLRKGDLVIALGFDETPRETKITIDFAIKQRIPVLGISHTPTS
                     EIARSSTLCLLGRRLPHSTQSLAASMSLLNALAIAVAARNKRQALRQLRRLDIIEQQY
                     SK"
     gene            2548..3693
                     /locus_tag="VF182_12235"
     CDS             2548..3693
                     /locus_tag="VF182_12235"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6437895.1"
                     /translation="MSDATYFSADSHVNEPPEAWERIPKQLRTHGPHFVQDPAGRKGL
                     YMVFDGHEPDPVGMTFTAGVQKEAGGVRKMIESFRWEDWRGPWDPLARLSDMDLDGVR
                     MEVLYPSMARNFYSLKGEEVPLQQAGLRSYNDWMRDYCLAAPDRLIGLGLLSALDIQW
                     SIDEMKRCLKLGHKGVVLPSGLPDGMSYSDPEFDSLWAAAQELDCPIHFHVNILQGRD
                     RMAARLKVITKLQQGRNAVRRGILEPLALLTDLVFGGVLNRFPKLRFVFAEYELSWLY
                     PFFTKMDGSVTRARSEAPESPTIEALPSESIRRQVYTTFQDDRVGVLGSEALNLVDNV
                     MWASDYPHGGATWPHSKDIIKAQFRGVSEEIKEKLTWKNAAKFYGVE"
     gene            3716..>4092
                     /locus_tag="VF182_12240"
     CDS             3716..>4092
                     /locus_tag="VF182_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437896.1"
                     /translation="MKWFRSIFTAFLVGAVISGDSVFAQTEAFYKGKTLQILVGSGPG
                     AGNDLKTRIFARHAPKYLPGTPNVIVENMPGAGGLRARNHLYNLVKPDGLTIAQIVRG
                     TELQEAIGDPGVKYKADRFNWIG"
BASE COUNT         1028 a         1026 c         1055 g          983 t
ORIGIN      
        1 gaaagaccga caagaatctt cttctgcgct tgagccgtgc cggcctcaag ccaaacgatg
       61 agaaagacag ccgcaataaa gagctttcgc agggcactct tgatgatcat gctttaaagt
      121 ttgctccgag gtcgctactt aaaagtccaa cgcgcatggc cgacgcccaa tgcagcagcc
      181 cgaagtcgtt atccgcgatc aagggactcg tatcctggtt gctgttgatc accccgagct
      241 aaccaatggc atcggcgaat tgcttggtat cggtatactg ctggaaccgc accactctgc
      301 cgtgggtcac gccccagatg tgggcgaatt gcgcgcggac ttctttaccg ctttctttgt
      361 gcctgccgct atagttgccg agcaccacaa tgtgattccc ggcatccagc cactcttccg
      421 gcatgacctt gaaatccgcc cattccgaag caagtctcat gaaaactcct tcgagcaccg
      481 cctgggggcc gcggtaagga ctccggtcgg cgtagatgaa gttttcagcc tcgcgccact
      541 cgatatgttg gtgcatttga ccaagcaccg aggtgacgtc tcccgtgctg aaggactcgt
      601 acaagccgcg aatcaccgcg atattatctt gtgacatctt gccctccttg aacgcacaga
      661 ttttcgctcg tgggccgtaa caatctcgtt atagatcctc cctagtggat atcgaaagca
      721 tccgcaacct cttgcgcagc tttcaaacac gatcgtcagt ctctcaaccg aagatcctgc
      781 catatctatt tcaccgcaat aagatgagaa gatcccgccg acaatctctg cgtctcgggc
      841 aaagcccgtc gcgatttcac ttcatgcaaa accagcgggc cccgactaaa gatgcgtgca
      901 gttttcgaga agaagaagca actgttagat tagatcgctc gttgtagaaa ggaactatat
      961 cgaatgacat caatgataag agtagcagtt ttgctagttg tagtttttgc ggtaaccggt
     1021 tgccaagctc tgactggacg aaccgccggt cggaatatcg atgacctgac gatcacggca
     1081 tcagtcaagg cacggcttgt cgcagacaag ctgtcaaatt ttactcgcat cgatgtggat
     1141 acggtgaatg cggtcgtttc cttaaccggg ctggttgaat ccgagtttga gaaacggcgg
     1201 gcggaagaca tcgcctcgtt agtcgaaggg gtggagaata taatcaacga tcttcaggtg
     1261 cagaaagaac ctgcagctaa gtcggagtga ccgtaactcc tgaaaaagca accgacagtg
     1321 accatttggc cttaaacaat cctggcgctg taaggtcatg gctccactga actttcgaaa
     1381 tctttggtcc tcaaaatgct gggatatcaa tccatccgca agggaattca gctgtacgag
     1441 tcgggctcta gtacgcgggt agtgatacgc gagcgcgaga acctgtatcc gaatggaaca
     1501 gacgagttat agttctctcg atggccatga actacagcac tgatgaaaaa tcagcggttc
     1561 atttggaata ctgctgctca attatatcca gccttcgaag ctgacgaaga gcttggcgtt
     1621 tgttccgagc cgccactgca atcgccaggg cattcagcag tgacattgaa gcggcaagag
     1681 actgagtgct atgaggtagg cggcgcccta acaggcagag cgtcgatgac cgcgcgatct
     1741 ccgaagtcgg cgtgtgactt atgcctaaca ccgggattcg ctgtttgatg gcgaaatcaa
     1801 tcgtgatctt tgtttctctt ggggtctcgt caaaacccag agctatgaca agatcgcctt
     1861 tcctcagctg cgccagttgc tccacaagag atgtgccgcc tgttagcatg atgaggcgag
     1921 catccaagcc gatacgtagc aagcgaaagt gtaaaaagta ggctaatgcg aaagccgcgc
     1981 gaaacccaat aataaagatc tttttggcac cgaagatcat tctgaccgct tgcgtaaatg
     2041 aggcctttgt taagctcgat tgcgtctcct tgatcaagtc tatgtcatgt tgaaacattg
     2101 actttggaaa gcttcgttct ccgaccgttg tgacggcatc ccctactcta gaggcagttc
     2161 cagaggtcct tttgtattcg gcttgaagct ccttgcgaag actaggataa cctttgtagc
     2221 ccagctcttt ggcaaaccta accagtgatg cttcactgac acctactttc tgcgatagct
     2281 cggtaatgga atcaaacgat gctgccatgc cattgttggc aatatagtcc gccaccttac
     2341 ggtggttctt actgaggctg gaataacggt cctgaattct ttcaatcagt tccaagtagc
     2401 ttctccgcat cgctgattag acgtttaaat tatagcttga cagcaaattt tcattttgat
     2461 accgctctga aaaaatattt gcaaacagtt attcgttagg attcagatcg actttgaatt
     2521 ctcatcaagc agtaaggagg catcaccatg agcgacgcca catacttctc ggcggactcg
     2581 cacgtcaatg aaccaccaga ggcatgggaa cggattccaa aacagctacg aacacacgga
     2641 ccacatttcg ttcaggatcc agcaggccga aagggcctgt acatggtttt cgatggccat
     2701 gagcccgatc cggtgggcat gacatttaca gccggcgttc agaaagaagc tggcggcgtt
     2761 cgtaaaatga tcgagagttt tagatgggag gattggcgcg gaccttggga cccattagcg
     2821 agattaagcg atatggacct ggacggagtc agaatggaag tcctttatcc gagcatggcc
     2881 cggaatttct acagcttaaa aggcgaggaa gtgccgcttc agcaagcggg acttcgctct
     2941 tacaacgact ggatgcgaga ttactgcctg gcggcgccgg ataggcttat cggcctcggg
     3001 ctcttgtctg ctctcgatat tcaatggtcc atcgacgaaa tgaagcgttg cctaaagctc
     3061 gggcacaaag gagttgtgct accgtcaggt ctcccggatg ggatgagtta ctcggatcct
     3121 gaatttgatt ctttatgggc ggctgcgcag gagttggatt gcccaataca cttccacgtc
     3181 aacattctgc agggtaggga tagaatggcc gctcgtctca aagtgattac caaacttcag
     3241 cagggacgaa acgcagtcag gcgtggaata ctcgagcccc ttgcattgct cactgacctg
     3301 gtctttggcg gagtattaaa ccggtttcca aagcttcgat tcgttttcgc tgagtacgag
     3361 ctgtcttggc tgtatccttt ttttacgaaa atggatggct cggtaacccg agcacgttca
     3421 gaggctcccg agtcgccaac cattgaggcg ttaccgagcg aatccatcag aagacaggtt
     3481 tatacaacct tccaggatga ccgagtgggc gttctcggtt cggaggctct caatctcgtc
     3541 gacaacgtaa tgtgggctag tgactatcct catggaggtg cgacgtggcc gcactccaaa
     3601 gacatcatca aagctcagtt ccgaggtgta tcagaggaga ttaaagaaaa gctcacctgg
     3661 aaaaacgcgg caaagtttta cggtgtcgaa taactcgtgg tcttcggaac gcgctatgaa
     3721 atggtttaga agcatcttta ctgcattcct agtcggagct gttatcagcg gtgattcagt
     3781 ctttgctcag acagaggcct tctataaggg taaaacgctg caaatcctcg ttggctccgg
     3841 ccccggcgcc ggtaatgatc tcaagacaag aatctttgcg aggcatgcac caaagtattt
     3901 gcccggaacc ccgaacgtga tcgttgagaa tatgcccggc gctgggggtc tcagagcgag
     3961 gaaccacctc tacaacctgg tcaagcctga cggattaacc attgcacaaa tagttagggg
     4021 taccgaactt caggaagcga ttggagatcc tggcgttaag tacaaagcag ataggttcaa
     4081 ctggatcgga aa
//
LOCUS       DASUVS010000217         5644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1399582, whole genome shotgun sequence.
ACCESSION   DASUVS010000217 DASUVS010000000
VERSION     DASUVS010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399582"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VF182_12245"
     CDS             <1..227
                     /locus_tag="VF182_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437897.1"
                     /translation="LTPRPPWSGYSLGLWPEEAAIQAGNAVAGNHSLNAEFSSKHGIK
                     VPRGAKFMDLKKKYMAEEIEKFKKQAGETK"
     gene            316..1320
                     /locus_tag="VF182_12250"
     CDS             316..1320
                     /locus_tag="VF182_12250"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437898.1"
                     /translation="MKLIKALICAVIVAFICNPTGAGSQAKKIKVGYSSDAPGSLVTW
                     LAREAGIFAKNGLDADLVRTRTTIGVMALLSGELDFIQASGPVVLESALRGSDLVYIA
                     GGMATLDFIFMSQPEIKRAEMLKGGVVGLASIRGASLVATEFALQKLGLNTKDVKFIV
                     IGGTPERLISMRNKRLQATLLSPPTSIVAEREGFNVLADVGALGLPFLHNGIAASRRF
                     VRDNPDTARRYVKAQVEAVHLMKTDRKTSVAVLGKYLRQTANKEILERSYELSSTDDK
                     YSRKQYPSLPGIQTVLDAIADENPKAKSARPEQFVDTRFIKELDDSGYIDSLYKGKAK
                     "
     gene            1323..1829
                     /locus_tag="VF182_12255"
     CDS             1323..1829
                     /locus_tag="VF182_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437899.1"
                     /translation="MQETLAQSLVHSLRAAGIDFITYLPETRLSQIIPLLQSDSHFQL
                     APVGSEAEGVSIAAGAALGGKVVASYMEGTGVYVACYNILTVGVRYGVPMLLLVSYVG
                     SLEDHRNSFLYVQRGIKLIPQFEALDVQYQVIRNGDGLERKVKNGVRMMNALKQPVAL
                     LFTEDFTL"
     gene            1826..2425
                     /locus_tag="VF182_12260"
     CDS             1826..2425
                     /locus_tag="VF182_12260"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6437900.1"
                     /translation="MIRYDCFEWLAPLMTDQLVVTSLSGQRVEWGHLSKREADLNLGS
                     MGNALAVGIGLALALPHRKVLVFESDGSVLLSLFNLPTLANLNPGNLAVFVFDNQAYS
                     GTRISYPSATAGKTDLAGVAKASGIDHSVTVAELDDFKKEAGAALTEIGLRFVVCKIE
                     ESVGHRQITRSNVDLLETKYRFVRYLERTEGKPIFHGRG"
     gene            2434..2940
                     /locus_tag="VF182_12265"
     CDS             2434..2940
                     /locus_tag="VF182_12265"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6437901.1"
                     /translation="MKPELAHKTVAALKRAGIDFVSYIPETRLSEILPVMKADEAFTL
                     AAVSSEAEAVGIAAGAALGGKRAAVYMEGTGLFVSAYNLLTVGERYGVPMLLVVAFVG
                     SFDDQRNSFLFSHYGIKTMSLLKTIGIEHVVVESGEHIEARIDDAVRMMNALKLPVAL
                     LFTGEFTD"
     gene            3118..4086
                     /locus_tag="VF182_12270"
     CDS             3118..4086
                     /locus_tag="VF182_12270"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437902.1"
                     /translation="MKPRVCKITGFLVAFFALGFQSVEAKTIYIGVSNPDMSFLSGGV
                     AHFQGYFKDEGLDVDVLQMNANVSVAALAAGNIDYNLILQSVVTANLRGLPLKVTGIL
                     IERPNHAVVVHPSIQKFADLKGKKIAISSFGSLVDILARLTSAHFKLDPKSDVQYVAA
                     GSSSARLTQLQTGVVQAAFLTPPGNLRAEAAGFKTLLNVRDLFAFPVNGIGLHEQKLK
                     NDRDEVKKVTRALLRANRFILDNPKGAIKILAAWGRSKLDVAEEAYQYNAKNYSRNLL
                     VSRSTLENVIESTRWNVETKRNVAVDDVFDFTIVREILKEMGETPK"
     gene            4147..5142
                     /locus_tag="VF182_12275"
     CDS             4147..5142
                     /locus_tag="VF182_12275"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437903.1"
                     /translation="MLTTLSRKIYAAASSVVLCLLVILLWIDSLPAQTADKLIFSIPS
                     RSIAAIDLYIAKERGFFRGEGLDVDIVQIRGNVAIAAALSGQVQATNGVGTVIRALER
                     SELPMKILTVSLKRNLFWLVSRPEITSIAQLKGKTLGTTTLGGSQHSAAMRLIRKGGV
                     DEKDITVVVGGDVPAQLQSLVGRTIDAAALSPPTVILARDKYNMNVLGSAMEDLANLQ
                     NGTAVTEKFLREKRDVLKRVLRARMRANRYFVENERGTSEVLAKYQNVELPVAVESYR
                     LAKPAFTMNGIPSDKEIEEFLKSEAELLKLKEPVSPSKIFDFSLQREVNQELGVK"
     gene            5376..>5644
                     /locus_tag="VF182_12280"
     CDS             5376..>5644
                     /locus_tag="VF182_12280"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6437904.1"
                     /translation="MEQELAQKLVTSLKEAGIDVITYLPETRLSQILPLLRSDDSFKL
                     IPVSSEAEAITIGAGAALGGKQVAVYMECTGLYVSCYSILSIAKR"
BASE COUNT         1308 a         1507 c         1583 g         1246 t
ORIGIN      
        1 aattgactcc gcgcccgccc tggtccggat attcattggg cttatggccg gaggaagcgg
       61 caatccaggc cggcaacgca gtcgccggca atcacagcct gaatgccgag ttcagttcga
      121 agcacggtat caaagtgccc agaggcgcga agtttatgga tctcaagaaa aagtacatgg
      181 ccgaagaaat tgaaaaattt aaaaaacaag ccggtgaaac gaaatagcaa taggaagctt
      241 attattatgc cggcgtgttc gatatccgcc cgctgacaaa tagaacatga taattccggg
      301 tcagagttgc gcttgatgaa acttataaaa gcactgatct gcgcggtaat cgttgcattt
      361 atctgcaacc ctaccggcgc cgggtcccag gcgaagaaga tcaaggtcgg ctacagttcc
      421 gatgccccgg gttcgctggt cacatggctc gctcgtgaag ccgggatttt tgccaaaaat
      481 ggcttggacg ctgacttggt ccgcactcgc accaccatcg gggtcatggc gctcctttcc
      541 ggcgagctgg atttcattca ggcttcgggt cccgtcgttc tcgagagcgc tctcaggggc
      601 tcggacctgg tttacatcgc cggcggcatg gcaaccctgg attttatttt tatgagccag
      661 ccggaaatca aaagagccga aatgctcaag ggcggagtcg tgggacttgc ttcgattcgc
      721 ggtgcctctt tggtagccac agaattcgcg ctgcaaaaac ttggtttgaa tacgaaggac
      781 gtcaaattca tcgttatcgg cggcacgccg gagcggttga tctcgatgcg caacaagcgc
      841 ctgcaagcca cgcttctcag tccacccacc agcatcgtcg cggagcgcga aggattcaac
      901 gtgctcgccg atgtcggcgc tctgggattg ccctttctgc ataatggcat tgccgcatcg
      961 cgccgctttg tccgggataa tccggacacg gcaaggcgct acgtcaaggc gcaggtcgaa
     1021 gctgtccacc tgatgaagac cgatcgcaaa accagcgtcg ccgtgctcgg caagtatttg
     1081 cggcagaccg ccaataaaga aattctcgaa agaagttacg agctctcgtc cacagacgac
     1141 aaatattcac gcaagcagta tccgtcattg ccgggaattc agacggttct agacgccatc
     1201 gccgatgaaa atcccaaggc caagtcggcg cggcccgagc aattcgtcga tacccgtttc
     1261 atcaaagaac tcgatgacag cggttacatc gatagcctct acaaaggcaa ggcaaaatag
     1321 ggatgcagga aaccctggcg caaagcctgg tgcattcgtt gcgggcggcg ggaatcgatt
     1381 tcattactta tctcccggaa acgcggctca gccagatcat accgttgcta caaagcgata
     1441 gccatttcca actggcgccg gtgggcagcg aagccgaagg cgtgtcgatc gccgccggcg
     1501 ccgctctcgg cggcaaagtc gtcgcgagtt atatggaagg aaccggggtt tacgtcgctt
     1561 gctacaatat cttgaccgtg ggcgttcgct acggcgtgcc gatgttgctg ctggtaagct
     1621 acgtcggcag tctcgaagac caccgaaaca gctttcttta tgtccagcgc ggcatcaagc
     1681 tgatcccgca attcgaagct ctcgatgtgc agtatcaggt gattcgcaat ggcgacgggc
     1741 tcgagcgtaa agttaaaaac ggtgtgcgca tgatgaacgc gctcaaacag ccggtcgcct
     1801 tattgttcac agaggacttt actttatgat tcggtacgat tgtttcgaat ggctggcgcc
     1861 gctcatgacc gaccaactgg tggtcacgtc gctgagcggc cagagagtcg aatgggggca
     1921 tctgtcgaag cgagaggccg acctcaacct cggctcgatg ggaaatgccc ttgcggtcgg
     1981 catcggcctg gcgctggcgc tgccgcaccg gaaagtgttg gtcttcgaat ccgatggcag
     2041 cgtgctgctt tcgctcttca acctgccgac tttggccaat ctgaacccgg ggaatctggc
     2101 ggtattcgtt ttcgacaatc aggcttacag cggcacgcga atcagctatc ccagtgccac
     2161 agcgggcaaa acggatttgg cgggagtggc gaaagcctcg ggcatcgacc attcggttac
     2221 cgtagcggaa ttggacgact tcaaaaaaga agcaggcgcc gctctcacgg aaatcggctt
     2281 acgtttcgta gtctgtaaga tcgaagagag cgtcggccac cggcaaatta ccagaagcaa
     2341 tgtcgacttg ttggaaacaa agtatcgttt cgtgcgttat ctcgaacgga ccgaaggtaa
     2401 accgattttt catgggcgtg ggtagcggtg attatgaaac ccgagttggc tcataaaacg
     2461 gttgccgcgc ttaaacgcgc cggaatcgat ttcgtcagtt acattcccga gaccagattg
     2521 agcgagatcc ttccggtgat gaaagcggac gaggccttta ccctggctgc ggtatcgagt
     2581 gaagcggaag ctgtcggcat tgccgccgga gccgctttag gcggcaaacg cgcagcagtc
     2641 tacatggaag gcaccggcct gtttgtctcc gcttacaacc ttcttaccgt cggcgagcgc
     2701 tatggcgtgc cgatgcttct ggtagtagct ttcgtcggca gcttcgatga tcagcgaaac
     2761 tcgtttttgt tttctcacta tggcatcaaa accatgagcc ttctaaagac catcggcatc
     2821 gaacacgtcg tggtcgaatc cggggagcat atcgaggcgc gaatcgacga cgccgtgcgc
     2881 atgatgaatg ctttgaagtt gccggtggcg ctgttattca ccggcgaatt taccgattaa
     2941 caggctacag gctgctgaaa agcctcatag gcttcgttgc gcttgcccga ctcgctcaac
     3001 gtactcacaa cgtacgcttc cgctcgtcag tcatcgcgcg cctcgcctat gagatctttt
     3061 gagcagcctg caggaacttg ttgcttatta catcaacttg caacgggaga atgaaagatg
     3121 aaacctcgag tgtgtaagat aaccggcttt ttagttgctt tctttgcttt aggatttcag
     3181 agcgttgaag ccaaaactat ttatatcggt gtgtccaatc ccgacatgtc gtttctgagc
     3241 ggcggtgtcg ctcatttcca aggctacttc aaagacgagg gactggacgt cgacgtgctg
     3301 cagatgaacg ccaatgtgtc ggttgcggcg ctggcggccg gcaatatcga ttacaatctt
     3361 attttgcaat cggtggtcac cgcgaatctc cgcgggctgc cgctcaaagt taccggcatt
     3421 ttgatcgaac ggccaaacca cgccgtggtg gttcacccga gcattcagaa gtttgctgat
     3481 cttaaaggca agaagatcgc gattagctcg ttcggctctc tggttgatat cctcgcccgg
     3541 ctgacttcgg cacacttcaa actcgaccca aaaagcgatg tccagtacgt tgccgccggc
     3601 agcagctcgg cgcgcctgac gcaattgcaa accggcgtcg ttcaggcggc atttctaacg
     3661 ccgccgggaa atctgcgggc cgaagccgcc ggcttcaaaa cattattgaa cgttcgcgat
     3721 ctgtttgctt ttccggtgaa cggcatcgga ctgcacgagc agaagctcaa aaacgatcga
     3781 gacgaagtca aaaaagtaac ccgcgctctg ctgcgggcca accgcttcat tctggataat
     3841 cccaaggggg cgatcaagat tttggccgct tggggcagat ccaaactgga tgtcgccgag
     3901 gaggcctatc agtacaacgc caagaactat agccgtaatc tgctggtgtc ccgctcgacg
     3961 ctcgagaatg tcatcgagag cacgcgctgg aatgttgaaa ccaagaggaa cgtcgcggtg
     4021 gatgacgtat ttgattttac catcgtgcgc gagatcttga aggaaatggg cgagaccccc
     4081 aagtagccgc ggcctgtcac tcaccgtgat catccaggtg acaacatcac aatacccgag
     4141 gcttccatgc tgactaccct tagtcgaaaa atctatgccg ctgccagctc tgtggttctg
     4201 tgcctgctcg tgattttatt gtggatagac tcgttgccgg cgcaaaccgc cgacaagctg
     4261 attttctcga tcccgagccg cagcattgcc gccatcgatc tttatatcgc caaagagcgg
     4321 ggatttttcc gcggcgaagg cctcgatgtc gacatagttc aaattcgcgg caacgttgcc
     4381 atcgccgcgg ccttgagcgg gcaggttcaa gctaccaatg gtgtcggcac ggtgattcga
     4441 gctctcgagc gcagcgagct gccgatgaag attctaactg tcagcctcaa gcgcaattta
     4501 ttctggctcg tgtccaggcc ggaaatcacc tccatcgccc agctcaaagg caaaaccttg
     4561 ggcactacca cattaggcgg ctcacagcat tcggcggcga tgcgtctgat ccgcaaaggc
     4621 ggcgtcgacg aaaaggatat tactgtagtt gtcggcggcg acgtcccggc tcagctgcaa
     4681 agtctggtcg gcagaaccat cgatgccgcg gcgctttcgc cgccgaccgt tatcctggcc
     4741 cgcgacaagt acaacatgaa cgtgcttggc tcggcgatgg aagatttggc caaccttcag
     4801 aatggcacgg cggtgacgga gaagttcctg cgcgagaaaa gggacgtgct gaagcgcgtc
     4861 ctgcgcgcgc gcatgcgggc gaaccgctat tttgtggaga acgagcgtgg caccagtgaa
     4921 gtcctggcaa aatatcagaa cgttgaattg ccggtggcgg tggaatccta ccgcctggca
     4981 aaaccggctt tcactatgaa cggcatccct tctgataaag agatcgaaga gtttctcaag
     5041 tccgaagccg agcttctcaa actgaaggag ccggtttcgc cgtcgaagat tttcgatttc
     5101 tcactgcagc gcgaggttaa ccaggagctt ggagtcaagt gaggaggaat gattgagata
     5161 acgttggagt actgccgcgg ggtctgtgaa gttcgttgat acaattcagc catcactata
     5221 ggcaagcggg gaaagttacc gtcacctgga aagccaggcc ccctctttgg ctcggcctta
     5281 gcaaagggga gagtagtggg ggttgatctc cactttggcg agtatgacgc ggagccgtca
     5341 aattctttct aaactttaga taagacacta gtcccatgga acaagaactg gcgcaaaaac
     5401 tggtgacgag cttaaaggaa gccggcatcg acgtgatcac ctatctgccg gaaaccagac
     5461 tcagccaaat tctgccgctg cttagaagcg acgattcgtt caagctcatt ccggtttcca
     5521 gcgaagccga ggcgatcacc atcggcgcgg gcgcggcttt gggcggcaag caagttgcgg
     5581 tttacatgga atgcaccggg ctctatgttt cctgctacag tatcttatcg atcgcaaaac
     5641 gttt
//
LOCUS       DASUVS010000218         3423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1618866, whole genome shotgun sequence.
ACCESSION   DASUVS010000218 DASUVS010000000
VERSION     DASUVS010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618866"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VF182_12285"
     CDS             complement(<1..243)
                     /locus_tag="VF182_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437905.1"
                     /translation="MKRKPLFFCTIGAISIAVCSTAPAQQQRKIPHVGYLTISSLSSN
                     VARNEAFRRGMQELGYVEGKRLLSMRAVSCPTDTTPL"
     gene            complement(284..1312)
                     /locus_tag="VF182_12290"
     CDS             complement(284..1312)
                     /locus_tag="VF182_12290"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437906.1"
                     /translation="MRMMSSHWSIVGEIRGRKMKSKMILICLLATFLLTISSTQAQQP
                     KISRVGILFVGGRDQPHLEALKKGLSELGYTEGKNIILEYRYAEGRYDRLPELAKELV
                     NGGVDVIVTTTSVSAQAVRKATKTIPIVMTSGNPVEQGLADSLAKPGGNVTGLTVLLS
                     DLSGKRVELLKETFPKINHVATLWSSRDALSSVAFKETQGAATAFAIQLSSMDLRNAE
                     DIDKAFAELPKKHVDAILVATSNPLVTFHSKEIVELALKHRLPGMYPTRQFAEEGGLM
                     AYGPLISHLYYRAATYIDKILKGAKPADLPIEQPTKFEFIINLKTAKQIGLTIPPNVL
                     ARADRVIR"
     gene            complement(1322..1513)
                     /locus_tag="VF182_12295"
     CDS             complement(1322..1513)
                     /locus_tag="VF182_12295"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX6437907.1"
                     /translation="MSYGVDLADLQRRAAIYVDKILKGAKPADLPVEQPTKFELVINL
                     KSAKQNRPHNPAERARAGG"
     gene            complement(1751..2725)
                     /locus_tag="VF182_12300"
     CDS             complement(1751..2725)
                     /locus_tag="VF182_12300"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437908.1"
                     /translation="MTPRFRLYFLALILLTWIPVIHAQQPKSFHRIGILRVGKPPDPF
                     IDAFRQGLQEMGYTEGQNIVIEYRWLQREDQLNDAAEELVRLPVDIVVATTTPAALAA
                     KRATRSIPIIAPVMGDPIGSGLMKTFAHPGGNVTGLTNLAPELWPKRLELLQETVPKL
                     HRVAMLWNTSNPAMALGAKETRVSAGPMKIMIQDRGAKNPNELENVFVALKNERPDGL
                     LVMIDPFTNRHAREIIEFASSVQLPAMYDEKSLSERGGLLSYGESRGELFRRAASYVH
                     KILRGTKPADLPVEQPMKFELVINLNTAKQIGLTIPPNVLARADRVIK"
     gene            complement(2825..2956)
                     /locus_tag="VF182_12305"
     CDS             complement(2825..2956)
                     /locus_tag="VF182_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437909.1"
                     /translation="MDRADLPVEQPTKFELVVNLKTAKQIGLLIPPNVLARADRVIR"
     gene            complement(3065..>3423)
                     /locus_tag="VF182_12310"
     CDS             complement(3065..>3423)
                     /locus_tag="VF182_12310"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX6437910.1"
                     /translation="FSSNLPTIQQVRFCCCPGREVVFGPSAVTVAIQHRLPTMASQTV
                     IADAGGLMTYGAPVTDISKRAATYVDKILKVAKPGDLPIEQPTNFDYVINLKAAKQIG
                     LTIPPNVLARANRVIR"
BASE COUNT          780 a          904 c          898 g          841 t
ORIGIN      
        1 cagtggagtc gtgtccgtag gacatgagac cgccctcatg gacaaaagcc ttttcccttc
       61 tacataaccc agttcctgca tcccccgacg gaaggcctcg ttgcgggcga cgtttgaaga
      121 aagggacgaa atggtcaagt atcccacgtg cgggattttt ctctgttgct gcgccggcgc
      181 ggtcgaacaa accgcaatgg agatagcgcc gatagtgcag aagaaaagtg gttttctctt
      241 cattttcaca atccaaacgt caagtttaaa atctaataca gcatcatcga attacccggt
      301 ccgctcgcgc cagcacattc ggcggaatcg tgaggccgat ctgctttgcg gtcttaagat
      361 taatgatgaa ctcaaacttt gtcggctgct cgatggggag atcagcgggt ttggcgcctt
      421 tgaggatctt atcgatgtag gtggcggctc tatagtagag atggctaatg agcggcccgt
      481 aagccattaa accgccttcc tcggcgaact gccgcgtggg atacatgcct ggaagccgat
      541 gcttcagcgc gagctccacg atctctttgg agtgaaaagt cacgagcgga ttggacgtcg
      601 ctaccaggat cgcatcgacg tgcttctttg gcagctctgc aaaggctttg tcgatgtcct
      661 cggcgttgcg tagatccatc gacgataact gtatggcgaa agctgtcgcc gcaccctgag
      721 tttctttgaa ggcgacgctt gataacgcgt cgcgcgaact ccacaacgtc gcaacatgat
      781 taatctttgg gaaggtttct ttgagtagct ccacacgttt gccgctcaaa tcggagagta
      841 atacggtcag gccggtgacg ttgccaccgg gcttggccag gctatcggcg agtccttgct
      901 ccaccggatt tccgctggtc atgacaatcg gaatcgtctt tgttgccttg cgcacggcct
      961 gcgcgctgac ggatgtcgtg gtcacgatca cgtcgacacc gccgttaacg agttccttgg
     1021 caagctccgg caaacgatcg tatcggccct cggcataccg gtattccaag atgatattct
     1081 tgccttcagt gtaaccaagc tcactgagac cttttttcag tgcttctaga tggggctgat
     1141 ctcttccgcc gacgaaaaga atgccaacgc gtgaaatctt cggctgctgc gcctgggtgg
     1201 acgaaattgt cagcaggaaa gttgccaata gacagatcag gatcattttg ctcttcattt
     1261 tccgcccgcg gatttcgccg acaatcgacc agtgactact catcattctc atttgatcac
     1321 cctatccgcc cgcgcgagca cgttcggcgg gattgtgagg ccgattttgc tttgcggatt
     1381 taaggttgat caccagttcg aacttggtcg gttgctcgac tggcagatcg gcaggcttgg
     1441 cgcctttgag aatcttatca acatagatgg cggcacgtcg ttggagatca gcgaggtcaa
     1501 cgccgtagct catcaggctg ccgcttcgac gaaattccct ctgacgtaca cggctggcaa
     1561 cttgctcgcg gtcgcatgct ccgctagtgc ttttcgcctg acattgaata ccgggccacc
     1621 taacacgagg actcgtagaa gtggggtcta ctattacgaa gaactttctt cggtgcagcg
     1681 cctgagctga aaagctaata accaagagca taacgcctag cgcaaaagcg aatgattctg
     1741 ccgacggaaa tcacttgatt acccgatccg cccgcgccaa tacattcggc gggatcgtta
     1801 acccaatctg cttggcagtg ttgagattga ttaccagctc gaatttcatc ggctgctcca
     1861 caggaaggtc agcgggcttc gtccctcgca aaattttatg cacgtaacta gcggctcgtc
     1921 ggaagagttc gccgcggctc tccccatacg aaagcaggcc acctctctca ctcaagcttt
     1981 tttcgtcgta cattgccggc aattggacgc tcgaggcgaa ctcgataatc tctctcgcat
     2041 ggcgatttgt gaacggatcg atcatgacga gcagtccgtc cggtcgttcg ttcttcagag
     2101 ctacgaaaac attctccagt tcgttcggat tctttgcgcc gcgatcttga atcatgattt
     2161 tcatgggacc ggccgaaact ctcgtttctt tcgcaccgag cgccatggcg ggattactcg
     2221 tgttccacaa catcgctaca cgatgaagct tcggcacggt ttcctgtagc agttccaaac
     2281 gtttgggcca tagctccgga gccaggttgg tgagacccgt cacattgcct ccaggatgag
     2341 cgaaagtctt catcagtcca ctgccaatag ggtcgcccat aaccggagcg ataattggga
     2401 tcgatcgtgt tgcacgctta gctgccaagg cggccggcgt ggtagttgcg acgacgatat
     2461 caactggcag acgaaccaac tcttcagctg catcattgag ctggtcttcg cgctggagcc
     2521 agcgatattc aatgacgata ttttgacctt cggtatagcc catttcctga agaccctgcc
     2581 tgaacgcatc gatgaaagga tcgggtggtt ttccgactct gaggatgccg atcctatgga
     2641 agcttttcgg ctgttgggcg tggatcaccg gaatccaagt gagaagaata agagccaaaa
     2701 aatagaggcg aaaccgtggt gtcatcttac ctcccgtaaa gcgttaacga acagcgatgc
     2761 ttcttgcggc gtcatgttga tccgtatcga ggcgggctgg atcttattcc acagcgcagg
     2821 acagttaccg gatcactcga tcagctctgg ctaaaacatt cggcggaatg aggagaccaa
     2881 tctgcttggc ggttttgaga ttaaccacca gctcgaattt tgttggctgc tcgaccggaa
     2941 gatcggctcg atccattctc gatttccgat tttagatttc cgattagcgc gaagtcagaa
     3001 tcaaagactc ggacccaaca cgatttctcg agtaaagttt cttccttcaa tcgaaatcta
     3061 aaattcatcg gatcactctg ttcgctcggg ctaggacgtt cgggggaatg gtcaaaccga
     3121 tctgcttggc ggctttcaaa ttaatgacgt aatcaaaatt cgttggctgc tcgatcggca
     3181 gatcacccgg cttggctact ttaagaatct tatcgacata agttgcggca cgtttagaga
     3241 tgtcggtaac gggagcccca taagtcatga gcccaccggc atcagcgatt acggtctggc
     3301 tagccatagt aggtaggcgg tgttgaatgg caactgtcac cgccgaagga ccaaagacaa
     3361 cttcgcggcc cgggcaacaa caaaaccgca cctgctgaat cgttggtaag ttgcttgaaa
     3421 aca
//
LOCUS       DASUVS010000219         8407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1837443, whole genome shotgun sequence.
ACCESSION   DASUVS010000219 DASUVS010000000
VERSION     DASUVS010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1837443"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2324
                     /locus_tag="VF182_12315"
     CDS             <1..2324
                     /locus_tag="VF182_12315"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6437911.1"
                     /translation="LLETFADQAVIAIENIRLFQELKESLEQQTATSEILGVIASSPT
                     DIQPVLDAVAKNAARLCEATDAQIRLIEGDGHRLVASFGTLPAPEFRVTSPENPFSRA
                     ILKRETVHIHDVQEEYGHPAASGARTLLSTPMLREGTPIGLINIRRTEIRPFSERHIR
                     LLETFAAQAVIAIENVRLFKELQERNAELREALEHQTATSEVLGIISRSPTDVQPVLD
                     AIVESAARVCGIDDVALRLREGNTMVMRAHFGPIPIPTSRVEISIDEPWYRWMREHGA
                     LQVPDVREAQNDFPTMGFGGDWRSILFIPLRQQGELAGTLIARRTVVRPFTPAQVKLL
                     ETFADQAVIAIENVRLFQELDARTRELARSVEELKALGEVGQAVSSTLDLLTVLSTIV
                     ARAIQLSGTDCGVIYEYDEAAEVFHLRASHRMEEELVEAIRAAPLRVGEGYTGQAALV
                     RAPVQVSDLRNEQEVRLMRIRPISDRLGYRSLLTVPILLEDRLMGALTVWRKRAGTFT
                     AELINLLQTFATQSALAIQNARLFREIEEKSRQIEAANRHKSEFLANMSHELRTPLNA
                     IIGFSEVLGERLFGELNEKQAEYTEDILSSGRHLLSLINEILDLSKVEAGRMELELAP
                     FDFPLAIDNARTFVRERATRHGITVDVKIDERLGEFVGDERKIKQILLNLLSNAVKFT
                     PEGGRIGINARQSNGAIEISVSDTGIGIAPEDQPRIFEEFRQVGSDYEHKREGTGLGL
                     TLAKKFVELHGGRIWVESELGKGSTFTFSLPHH"
     gene            complement(2574..2960)
                     /locus_tag="VF182_12320"
     CDS             complement(2574..2960)
                     /locus_tag="VF182_12320"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX6437912.1"
                     /translation="MTLLLDTHFLIWLVLESKRLANFPWLDRYRPWGVSPVSFLEILF
                     LAEVGRLSVSNPEFTSTVMKDRRFIIDDISLETLVGHALRFDWTRDPFDRLLVAHSLA
                     RRVALCTTDRTIRSRHRLVPSELNEE"
     gene            complement(2957..3214)
                     /locus_tag="VF182_12325"
     CDS             complement(2957..3214)
                     /locus_tag="VF182_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437913.1"
                     /translation="MKASEARKRWFQLLDDALRGEVIAIERNGQRIVLRREEPAQTKS
                     AKASEQYKKILKVPRADEADQWSWEWRGPGRRLVPRRRSRK"
     gene            3250..3375
                     /locus_tag="VF182_12330"
     CDS             3250..3375
                     /locus_tag="VF182_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437914.1"
                     /translation="MKQHKNSDPERWGTTNEGVRVALEEIENSKFEMDVSLKEKS"
     gene            3474..>8407
                     /locus_tag="VF182_12335"
     CDS             3474..>8407
                     /locus_tag="VF182_12335"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6437915.1"
                     /translation="MPPTKRPPPKNKRSSHETTDAKLRRENKQLRRERDEALEQQAAT
                     TEILRVIASSPTDIQPVLETVAQSAARLCEAQDAVIYRLDGDVLRRAAICGLIPMRET
                     PLRLSRGTTSGRAVVDRQTIHIHDIDAESETEFPDIDKTGREGGSRARTRLAVPLLHE
                     GVALGTILIRRMEARPFTDKQIALLKSFADQAVIAIENARLFQELTEALEQQIATSEI
                     LRVIASSPTDIQPVLNAVAENAARLCDASDAVIFRYDSDLARSVASYGSIPLARAMGE
                     EHRTTRGSPPGRAMLDRKTIHVHDIVAEFDTEFPESRARHQISGSRTVLATPLLREGA
                     SIGAIMIRRTEVRPFSDKQIKLLETFADQAVIAIENVRLFQELQTRNRDLTEALEQQT
                     ATGEVLRVIASSPTELQPVLDTLIANAVRLSGASRGHVRRYDGEFHRVVAHYGETPEM
                     IAVLSANPLPASSEVPAARALMGRQPVHILDVLEERGAHLDLARQTGVRTMLAVPLMR
                     EGNHIGSLTIWRDLVEPFTDRQIELVKTFADQAVIAIENVRLFNELKESLEQQTATSE
                     ILGVIASSPTDIQPVLDVVAENAARLCDASDAAIWRADSDKFWLVASHGSIPIHRREE
                     FRSMNRRGPLGRAMIDRETIHIHDLSSSEAQAEFPAAWTISQSVGVRTDLVTPLLREG
                     VAMGAIHIRRTEVRPFSDKQIALLKTFADQAVIAIENVRLFKEIQERNAELREALEHQ
                     TATAEVLAIISRSPTDVQPVLDAIVESAARVCGIDDVVLRLKDSDSMIPRAHFGSIPI
                     ARVKVSTDDLRFRWMQEHGTLHLPDYRAQNDFPKGDSVLGFRTFLSAPLRQHEKLIGV
                     LSARRMELRPFTPVQIKLLETFADQAVIAIENVRLFQELKEALEQQTATSEILGVIAS
                     SPTDLQPVLEAVAASAARLCDANDAIIHRREEGLARPVAFYGPIGFTRPAGQGFRPTR
                     SDPPGRAMLDGKTIHVQDIVAELDTEFPESRAHQQISGSRTILATPLLREGVSIGSIM
                     IRRTEVRPFTDKQVKLLETFASQAVIAIENVRLFKELQERNAELREALEHQTATAEVL
                     GIISRSPTDVQPVLDAIVESAARVCGIDDVLLRLREGDVMIARAHFGPIPIGYVGLSI
                     DEPNYRWIHQHGTLHVADVRDQNEFPTLGLAGGWRTLLSSPLRQKGELVGLLNARRIE
                     VRPFTPVQIKLLETFADQAVIAIENVRLFNELKEALEQQTATSEILGVIAGSPTDIQP
                     VLNTIAEHAGRICGTPDVSIIRVEENAYRIVSTYGSAPNWTTGEIRPLDRGSVVGRAI
                     VDRQTLHIHDVRAVEDDFWRVKNNAVLQGLRTMLAVPLMREGLAIGAIGIRRTEVHPF
                     TDKQIALLKTFADQAVIAIENVRLFKELDERNKELREALEHQTATSEVLSIISRSPTD
                     VQPVLDAIVESAAKVCGIDDVHLRLRAGNAMVSRAHFGTIPILRPEIPIDESNFRWVR
                     EHGTLHVPDVSKQSKFQQLGSERHFRTHLVVPLRQQGDFIGLMGARRTEVRPFTPAQI
                     KLLETFADQAVIAIENVRLFQELKESLDQQTATSEILGVIASSPTDIQPVLDVVAENA
                     AHLCEANDALIFRTDGDVMHMAAKYGPMPVAETMR"
BASE COUNT         1976 a         2282 c         2391 g         1758 t
ORIGIN      
        1 agctgctaga gactttcgct gaccaagccg ttattgccat cgagaacatc cggctgttcc
       61 aagagctgaa ggaatcgctg gagcagcaaa ccgcgacgag cgagattctt ggcgtcatcg
      121 ccagctctcc gaccgacatt caaccggtat tggacgctgt cgctaaaaat gcggcccgat
      181 tgtgcgaagc aaccgatgca cagattcgac tcattgaagg tgatggccac cgattagtgg
      241 catccttcgg aactctaccc gctccggaat ttagagttac gagcccagaa aaccctttca
      301 gccgagcgat tcttaaacga gagacggtac acatccacga tgttcaggaa gagtatggtc
      361 acccggcagc ctctggagca cgcacacttc ttagtactcc gatgctaaga gaaggcactc
      421 ctatcggtct gatcaacatc agacgaacgg agattcgtcc tttttcagag agacatatta
      481 gattgctcga aaccttcgcc gcccaggcgg tcatcgcaat cgagaacgtg cggctgttca
      541 aagaattgca ggagcgcaat gctgaactgc gtgaggcgct ggagcatcag acggcaacgt
      601 cggaggtgct cggcatcatt agccgctcgc ccacggatgt gcagccggtg ctcgacgcca
      661 tcgtcgagag cgcggcgcgg gtttgcggaa tcgacgatgt ggcgctacgg ctccgcgagg
      721 gaaacactat ggttatgcgg gctcatttcg gtcccatacc cataccaact agccgcgtcg
      781 agatcagtat tgatgagcca tggtatcgct ggatgcgcga gcacggcgcg ctccaggttc
      841 ccgacgtccg cgaggcacag aacgatttcc cgacaatggg ttttggcggc gactggcgca
      901 gcatcttgtt cattcccctt cgtcagcagg gggaactcgc tggaaccctg atcgcacgtc
      961 gcaccgtggt gcgtcccttt acccccgctc aggtcaaact gctagagacc ttcgccgacc
     1021 aagccgtcat cgccatcgag aacgtgcgct tgtttcaaga attggacgca cggacgcgcg
     1081 aactggcgcg atcagtagaa gaactcaagg ccttgggaga agttggccag gcggtaagtt
     1141 cgacgctgga tcttttgacc gtgctcagca cgattgtggc acgcgcgatt cagctgtcgg
     1201 gaaccgattg cggagttatt tatgagtacg atgaggcggc agaggtgttt cacctgagag
     1261 ccagccatcg tatggaagag gagctggttg aagctatccg cgccgccccc ttgcgggtcg
     1321 gggaaggcta tacgggacaa gcggcgctag tgcgggctcc ggtccaagtc agcgatctgc
     1381 gcaacgagca agaagtcagg ctgatgcgga ttagaccgat ctcagaccgg cttggttatc
     1441 gatctctttt aacggttccg atcctgcttg aagatcggct catgggcgcc ttgacggtct
     1501 ggcgaaagcg ggctggaaca ttcaccgcag agctgatcaa tcttcttcaa acctttgcca
     1561 cgcaatcggc tctcgccata cagaacgccc ggctgttccg cgagatcgaa gagaagagcc
     1621 gacagatcga agctgccaac cggcacaagt cagaatttct cgccaacatg tcgcacgagc
     1681 tgcgcacgcc gctcaacgcc atcatcggtt tctccgaagt cttgggcgag agacttttcg
     1741 gcgagctcaa tgagaagcaa gccgagtaca ccgaggacat tctttcctcc ggccgccatc
     1801 ttctctcgct catcaacgaa atactggatc tctccaaggt cgaagccggc cgcatggagc
     1861 tggagctggc accgtttgat ttcccattgg cgatcgacaa tgcgcgcacg tttgtgcggg
     1921 agcgcgcaac gaggcatggc attacagtcg atgtaaaaat tgacgagcgc cttggcgaat
     1981 ttgtcggcga tgagcgcaag ataaaacaga tcctgctcaa tctgttgtcc aacgcggtaa
     2041 agttcactcc ggagggggga cggatcggga ttaatgccag acagagtaac ggtgctatag
     2101 agatctcggt aagtgacaca gggatcggca tcgcgcctga ggaccagccc aggatcttcg
     2161 aagagtttcg tcaggtgggc agcgactatg agcacaagag agaggggacg ggacttggtt
     2221 tgaccttggc gaagaagttc gtcgagctgc atggaggaag gatctgggtg gagagcgaat
     2281 taggcaaagg cagtaccttt accttctccc taccccacca ttaattttcg attttcgatt
     2341 ttggattacg gatctcggta tgaacgaagc agctttcatc ggacaaagca ggttgcttgc
     2401 ggaatcatcc gactcgtcga atcgctgcct aacgccgaac ggcagatgtt attggaaggc
     2461 aattacttcg ttctgggacg tcggttggtg ccaactaaag tgcggcatga caacctcgca
     2521 atattcgagg aagaacagat gagaagagct accgttatgg ttgacgtgaa tgctcattcc
     2581 tcattgagtt ccgatggtac aagccgatgc cgggaacgaa tcgtgcggtc agttgtacaa
     2641 agcgcaacac gtcgggccaa gctgtgtgcg acaagcaatc ggtcaaaggg gtcacgggtc
     2701 cagtcgaacc tcaaggcatg gcccacgaga gtttccagcg aaatatcgtc aatgataaat
     2761 cgtctatctt tcatgacggt gctggtgaac tcgggattac taacggaaag ccgtccaact
     2821 tcagccaaaa acaggatttc cagaaatgaa acaggagaca caccccatgg gcgataccga
     2881 tccagccaag ggaaattagc caaacgtttg gattcgagga cgagccagat tagaaaatgg
     2941 gtgtcgagaa gaagggtcac tttcttgacc gccgccgtgg aaccaggcgg cgccctggac
     3001 cacgccactc ccacgaccat tgatctgcct catcggcgcg cggcactttc aggatttttt
     3061 tatactgttc ggaagctttg gcgctcttgg tttgcgcggg ctcttcccgg cgcagcacga
     3121 ttcgttggcc gttgcgctcg atcgcaatta cttcgccccg gagcgcatca tcgagcagtt
     3181 gaaaccaacg ttttctggct tcggatgcct tcacgtgctt cacatgtaca atgtacaaca
     3241 gagggtgcaa tgaaacaaca caagaattcc gatccggagc gctggggtac cactaacgaa
     3301 ggagtaaggg tagcattgga ggaaattgaa aactctaaat tcgaaatgga tgtttcgtta
     3361 aaagagaaat cgtagttttc accgccgagg cgcggaaaac atagagaagc aaaagagatg
     3421 ttgcctctgc gaactctgcg tctctgcggt tatatttgat ccgtaggtaa acgatgccgc
     3481 cgactaaacg acctccgccg aaaaataaac gttcatccca tgagacaacg gacgccaagc
     3541 tgcgccggga gaataaacaa ctaagacgcg agcgcgacga agcgttggag caacaggcgg
     3601 ccacaaccga gatcctgcgt gtcattgcca gctcgccaac agatattcag ccggtgctcg
     3661 aaaccgtggc gcaaagcgcc gctagactat gcgaggcaca agacgcggta atctatcgtt
     3721 tagatgggga tgttttgcgg cgagcggcga tatgtgggct gattcccatg cgggaaacgc
     3781 cgttacgact aagccggggc acaacgagcg gccgcgccgt ggtcgatcgg caaacaattc
     3841 atatccatga catcgatgct gaatctgaaa ccgaattccc ggatatagac aagacgggca
     3901 gggaaggcgg tagccgggct agaacgagac tcgcggtacc tttactgcac gagggtgtcg
     3961 cgctgggcac catcctgatt cgccgaatgg aggcccgccc gttcacagat aaacaaattg
     4021 cgctcctgaa atcttttgcc gatcaggcgg tgatcgcgat cgaaaacgcc cggctttttc
     4081 aagaactcac cgaagccttg gaacagcaaa ttgcgacgag tgaaatattg agggtcatcg
     4141 ccagctcgcc gaccgacatt cagcccgtgc tgaatgctgt agccgaaaat gccgcgcgac
     4201 tatgcgatgc aagcgatgca gtgatctttc ggtatgacag cgatctcgcg cggtccgttg
     4261 cgtcgtatgg atcgattccg ttggcaagag cgatgggaga agaacatcgc acgacacgag
     4321 ggtcgcctcc agggcgagcg atgcttgacc gcaaaacgat tcacgttcat gacatcgtcg
     4381 cagaattcga taccgagttt ccggagtcca gggcacggca tcagatcagt ggctcccgaa
     4441 cagtgcttgc cacgcctcta cttcgcgaag gtgcgtccat cggggcgatc atgattcggc
     4501 gcacagaggt tcgtccgttc tcagacaagc agataaagct cctggaaacc ttcgctgacc
     4561 aagccgtcat cgccattgag aacgttcggc tgttccaaga gctacagacc cgcaaccgcg
     4621 atctgactga agcattagaa cagcaaactg caaccggcga agtgttgcgg gtgattgcat
     4681 cctcgcccac agagcttcaa cctgtacttg atacgctgat cgcgaatgcg gttcggttgt
     4741 ccggagcaag cagggggcat gttcggagat atgatggcga gtttcaccgt gtagtggctc
     4801 attacggtga gacccccgaa atgattgccg ttctcagtgc caatcccttg ccggcaagct
     4861 cggaagtgcc agctgcgaga gcccttatgg gacgacagcc ggtccacatc ctcgatgttc
     4921 tagaggagcg cggtgcgcat ctcgatctgg cccggcaaac gggggttcga actatgctcg
     4981 cggtgccgct catgcgcgaa ggaaaccaca tcggtagtct cacgatctgg cgtgacttgg
     5041 tcgaaccatt tacggatcgg cagatcgagc tggtgaagac cttcgccgac caggcggtga
     5101 tcgcgatcga aaacgtgcgg ctcttcaatg agctcaagga gtcacttgag caacagactg
     5161 cgacgagtga aatattgggt gtgatcgcca gctccccgac tgacatccag ccggttttgg
     5221 atgtggtcgc agagaacgct gcacggttgt gcgatgcaag cgacgctgcg atttggcgtg
     5281 ctgacagcga taagttctgg cttgtagcct cgcatggatc gatcccaatt caccggcggg
     5341 aggaatttag atcgatgaat cgtcgtgggc ctctcggcag agcaatgatc gaccgagaga
     5401 ccatccacat ccatgattta tccagttcag aggcccaagc cgaatttccg gcggcctgga
     5461 ctatcagtca aagcgtcggc gtccgaaccg atctcgttac accgctgctt cgtgaaggag
     5521 ttgcgatggg ggcgattcac atacgccgta cggaggttcg tcccttctcg gacaagcaga
     5581 tcgcgcttct caagaccttc gccgatcagg cggtcatcgc catcgagaat gtgcgtctat
     5641 tcaaagaaat ccaggagcgc aacgcggaat tgcgcgaggc cctggaacat cagaccgcaa
     5701 cggccgaggt gctcgcaatc atcagccgct cgcccacgga tgtgcagccc gtgctcgacg
     5761 cgattgtcga aagtgcagcg cgggtttgtg ggatcgatga cgtggtgctg cggctcaaag
     5821 atagcgactc gatgattccc cgggcgcatt ttggttcaat acctattgcc cgcgtcaagg
     5881 tcagtactga tgacctacgc tttcgctgga tgcaggagca tggcacgctc caccttcccg
     5941 attaccgcgc ccagaatgat ttcccgaagg gcgactccgt cctcggcttt cgcaccttcc
     6001 tatccgctcc ccttcgccag catgaaaagc tgattggcgt actgagcgca cgtcgcatgg
     6061 agctgcgccc cttcacccct gtgcagatca aactgctcga aacctttgcc gatcaagctg
     6121 ttatcgccat cgagaacgtg cggttgttcc aggaactaaa ggaggcgtta gagcagcaaa
     6181 cggcaacgag cgaaattttg ggagtcatcg ccagctcacc gactgacctg cagcccgtcc
     6241 tcgaagcggt tgcagcaagc gcagcccggt tgtgtgacgc caatgacgca atcatccatc
     6301 gtcgcgaaga gggactagct cgtcccgtgg cgttttatgg tcctataggg ttcaccagac
     6361 ccgctggaca aggtttccgt ccgactagat cagacccgcc tgggcgagcc atgctggacg
     6421 gaaaaacaat acatgttcag gacatcgtag cggaactcga tactgagttt ccggaatcta
     6481 gagcacacca acagattagc ggttctcgca cgattctcgc cacgccgctt ctgcgtgaag
     6541 gcgtatccat cggctcgatc atgatccgtc gcacggaagt tcgccctttc actgataaac
     6601 aggtcaaact tcttgaaacg tttgccagtc aggcagtgat agccatcgag aacgtccggc
     6661 tgttcaagga attacaggaa cgcaatgctg aactgcgcga agcgttggag catcagaccg
     6721 caacggccga ggtgctcggc attatcagcc gctcgccgac agacgtgcag ccggtcctgg
     6781 atgccatcgt cgaaagcgcc gcacgggttt gtggaatcga tgacgtgctg ctgcggctcc
     6841 gcgaggggga cgttatgatc gcgcgggctc attttggccc catacctatc gggtatgtcg
     6901 gccttagtat cgatgagcca aactatcgct ggatacacca gcatggcacg ctccacgttg
     6961 ccgatgtccg tgaccagaac gaattcccaa cgttgggtct agccgggggc tggcgcaccc
     7021 tattgtcctc tccccttcgt cagaaggggg aactcgttgg attactgaac gcacgccgca
     7081 tcgaggtgcg ccccttcacc cctgtgcaga tcaaactgct cgaaaccttt gccgatcaag
     7141 ctgttatcgc catcgagaac gtgcggttgt ttaatgaatt gaaagaggca ttagaacagc
     7201 agacggcgac aagcgagatc ctgggcgtga tcgccggctc accgacggat attcagccgg
     7261 tactgaatac gattgctgag catgctggac gtatttgtgg aacgcccgat gtctccatca
     7321 ttcgcgtcga agagaacgct tatcgcatcg tttcgacgta tggctccgcc cccaattgga
     7381 cgaccggcga aatccgtccg ctggatcgtg gctccgtggt tggccgtgcg atagtcgatc
     7441 ggcaaacgct acatattcat gacgtccgag cagtcgaaga cgacttttgg cgcgtcaaaa
     7501 ataacgcagt actacagggc ttacgaacaa tgcttgcggt tccgttgatg cgcgaaggtc
     7561 tcgcaatcgg tgcgattggg attcgccgta ccgaagtcca tcccttcacc gacaagcaga
     7621 tcgctcttct aaagactttc gctgatcagg cggtgatcgc gatagagaac gtgcggctgt
     7681 tcaaagaact tgacgagcgc aataaagagt tgcgcgaggc cctggaacac cagactgcaa
     7741 cctcagaagt gttgagcatc attagccgct cgcccacgga cgtgcagccg gtgctggatg
     7801 ccatcgtcga gagcgccgca aaagtatgtg gaatcgatga cgtgcacctg agacttcgtg
     7861 ccgggaatgc gatggtttca cgcgctcatt ttggcacgat acctatactg cgcccagaga
     7921 tccccatcga tgagtcaaat ttccgctggg tccgtgagca tggcacgcta catgttcccg
     7981 atgtcagcaa gcaaagcaag tttcagcagc tcggttccga aaggcatttc cgcacacatt
     8041 tagtagttcc gctccgccag cagggagatt tcattggatt gatgggtgcg cgtcggaccg
     8101 aggtgcgtcc tttcactccg gcgcaaataa agcttcttga aacgttcgcc gatcaagcag
     8161 tcatcgctat tgagaacgtg cggctgttcc aagaattgaa agagtcgttg gatcaacaaa
     8221 ccgcgacgag tgaaatactc ggtgtcatcg ccagctcgcc cactgacata cagccggttt
     8281 tggatgtcgt tgcggagaac gctgcgcatt tatgcgaggc aaacgacgct ttaatcttcc
     8341 ggaccgatgg agacgttatg cacatggcgg cgaaatatgg accaatgccg gtggcggaaa
     8401 cgatgcg
//
LOCUS       DASUVS010000220         3087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1837653, whole genome shotgun sequence.
ACCESSION   DASUVS010000220 DASUVS010000000
VERSION     DASUVS010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1837653"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VF182_12340"
     CDS             <1..499
                     /locus_tag="VF182_12340"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEX6437916.1"
                     /translation="INQEYEEKTIIKQSRIPGAGNGLYAAVKINKGETIGELGGRLVS
                     DDDESHGNHYIASIPECAWEKTRPYKYINAKEHGGHVSRINFAPSEINGIETHLQNAT
                     IKQICEYPYFIYVAVRDIEPGEEIWSSYGPDYEYGHFMNIPQVRDFFCGLVKLDCRDR
                     FTFSH"
     gene            682..1749
                     /locus_tag="VF182_12345"
     CDS             682..1749
                     /locus_tag="VF182_12345"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX6437917.1"
                     /translation="MKIETIETTILEMPYTKPLITATNHFTVARGLLVKVVTDGGLPG
                     YGYSDLFPRTGETAETARHVIDAVLEPKVNGRELEDLARIRADLDHALTGNPRAKAAL
                     ECALYDALARSIHIPLNLLFGGRYRNEIRVIKMISVGQPEMMASEAKQVADEGLALKL
                     KMSGNISLDLSRVAKVRQAVGDDVFIKVDANEAYDAKTAIRLAKGLADHGVAIFEQPV
                     PRDQLGALWEVKKHAPIKIEADQSVRTVADAAYLIRERMVDSINTGIQKIGGIQEVRK
                     IAELCEQAGINCALSNTAGSMVGDAAAVHLAASMPGISPLCELGEFEVISGDPFFGLA
                     VEKGAIQVPEGEGLGVGLRGF"
     gene            1775..2482
                     /locus_tag="VF182_12350"
     CDS             1775..2482
                     /locus_tag="VF182_12350"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6437918.1"
                     /translation="MSQSEFAAAIDKIVGDYNDRLKWVSFSKDHLTRESAQVLIEQWS
                     NFTRHSRQCWAHVVGNCPIIEVRKFIVTENLYEEEAQEGHSHFEILVRMGMAVGLTRH
                     DIEFAKPLPTTVVAMNAWEAMTKNRSWYEGLAAKSVLERTNNPNCGNFSHHQADYWTR
                     QLKLSKEDTEFWWMHDSVDQIHGDGSLKLLEKYLTTDDQKQAALRAAEESMMAWTVYF
                     DGFYYEGLRRSGTSPLK"
     gene            2639..>3087
                     /locus_tag="VF182_12355"
     CDS             2639..>3087
                     /locus_tag="VF182_12355"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6437919.1"
                     /translation="MAKPQTKKSLETFNQELAEVHMTGQWIYEELLNRAIGGPRPRGD
                     AYLWPWQMVRGKLLEACDVLEESFTARRSVLFGNPGLNDNATTHTLLMGIQMIKPGEI
                     AWAHRHSLAAIRFVIKGDGKVFTVVDGEKCPMEPYDLILTPQWTWHD"
BASE COUNT          762 a          792 c          882 g          651 t
ORIGIN      
        1 aatcaatcaa gagtacgaag aaaagaccat aataaaacaa tcgagaattc ccggtgcggg
       61 caacggattg tatgcggcag taaagatcaa caagggcgaa accatcggcg aattgggcgg
      121 ccgtctggtc tctgatgacg acgagtccca tggcaatcat tacatcgcat cgattcccga
      181 atgcgcttgg gaaaaaaccc gtccttacaa gtacatcaac gcgaaagagc atggcgggca
      241 cgtcagtcgc atcaatttcg ccccaagcga gataaacgga atagaaactc acctgcaaaa
      301 cgcgacgatc aaacaaatct gcgaatatcc gtattttatc tatgtcgccg tgagagacat
      361 cgaacccggc gaggaaattt ggtccagtta cggcccggac tacgaatacg gccacttcat
      421 gaacattccg caggtgcgag actttttctg tggcctggta aagcttgact gccgcgatcg
      481 atttaccttc tctcattaga ttgcacgacg gcttgggatg gctaggtctt acagctcagc
      541 accgctgatc atgcgtgttc cttcttaaat gaacttatcg gcgccgaaaa cttcttttca
      601 gaagtggcga ctcttcgccc cgttgtgata tcggtcaggc gaggtttgtt ctaaactgca
      661 cgaatcgggg gcaagtagcc gatgaagatc gagaccattg aaaccaccat actcgagatg
      721 ccgtacacca agccgttgat cacggcgacc aatcatttca ccgtcgctcg cgggctgctt
      781 gtcaaagtcg ttaccgatgg cggccttccg ggctatggct attcggatct tttcccgcgc
      841 accggtgaga cagcggagac cgcgcgccac gtcattgacg cggtgctcga gcccaaggtc
      901 aatgggcgtg aactagaaga tttggcccgc atacgggcag acttggatca cgcgctgacc
      961 ggcaatccaa gagccaaagc cgccctggaa tgcgctttgt acgatgccct ggcgcgcagc
     1021 attcatattc cgttgaatct gctgttcggc gggcgttatc gaaatgaaat cagagtgatc
     1081 aaaatgatca gcgtcggcca gcccgagatg atggccagtg aagccaaaca ggtggccgat
     1141 gaaggactcg cgctaaagct caagatgagc ggcaatataa gcctcgacct gtcccgcgtc
     1201 gcaaaagtcc gccaggccgt cggcgatgat gtatttatca aggtcgatgc taatgaggcc
     1261 tacgatgcca agactgcgat tcgtctggcc aagggcttgg ccgatcatgg cgtggcgatc
     1321 tttgagcaac ccgttccgcg cgatcaactc ggcgcgctgt gggaagttaa aaagcatgcg
     1381 ccgataaaga tcgaggcgga tcaatcggtg cgcacagtgg ccgacgccgc ctacttgatc
     1441 agagagcgaa tggtggattc gatcaacacc gggatccaaa agatcggcgg catccaggaa
     1501 gtaagaaaaa tcgccgagct gtgcgaacag gccggcataa actgcgcgct atccaatacc
     1561 gcgggcagca tggtcggcga tgctgcggcg gtgcatctgg ccgcgagcat gccggggatc
     1621 tcgccgctgt gcgagctcgg agaatttgaa gttatttccg gagatccgtt ttttggcctc
     1681 gcagtcgaaa aaggcgcgat ccaggtaccc gaaggagaag ggctgggtgt gggtctgaga
     1741 ggattttaaa tgcggggagt tccggaggat cttcatgagc caatctgaat ttgccgcggc
     1801 gatcgacaag atcgtgggcg attataatga tcgtctgaag tgggtgtcat tttccaaaga
     1861 tcatctaacc cgtgagtcgg ctcaagtatt gatcgagcaa tggtccaatt tcacccgaca
     1921 tagccggcag tgctgggcgc atgtggttgg taactgtccg attatcgagg tgcgtaaatt
     1981 tattgtcaca gaaaatcttt atgaggagga ggcgcaggag ggccattcgc attttgaaat
     2041 tctcgtgcgc atgggaatgg ccgtagggct cacgcgacac gatattgagt tcgccaagcc
     2101 cttgcccacc acggtagtgg cgatgaacgc ttgggaagcg atgaccaaaa accgttcctg
     2161 gtacgagggc cttgcggcca agtccgtcct cgaacgaacc aacaatccga actgcggcaa
     2221 cttctcccat catcaggccg attactggac ccgccagttg aaactgtcca aagaggacac
     2281 cgagttttgg tggatgcacg actcggtaga tcagattcac ggcgacggct ctttgaaact
     2341 tttagagaaa tacttgacaa cggacgatca gaagcaggcg gcactgcgcg cggcggaaga
     2401 atcgatgatg gcttggacgg tctacttcga cggtttttac tatgaaggac tccgaagaag
     2461 cgggacgagc ccgttgaagt gaaacgtgtt ccaatcgttc aaaacgttca agccgttcaa
     2521 atcgttcaat cgcaatcccc ctccttcatc ctcccccacg accgggggag gaatagaggt
     2581 gggggttgga acgagctgaa cggttggaag gggagcgaag cgacaattag gagttaccat
     2641 ggctaagccg cagacgaaga aatcgttaga gacattcaat caggagctgg ccgaggtcca
     2701 tatgaccggt caatggatct acgaagaatt gcttaatcgc gccatcggcg ggccccggcc
     2761 gcgcggcgac gcctatcttt ggccgtggca gatggtgcgc ggcaaattgc tcgaagcctg
     2821 tgacgtgctc gaagagagct ttacagcccg ccgcagcgtt cttttcggta atcccggttt
     2881 gaacgataac gctaccacgc acacgctttt gatgggcatc cagatgatca agcccggcga
     2941 gatcgcctgg gctcatcgtc acagtttggc ggccatccgc ttcgtcatca aaggcgacgg
     3001 caaagtgttt accgtggttg acggcgagaa gtgtccaatg gagccctatg atttgattct
     3061 tacgccgcaa tggacctggc acgatca
//
LOCUS       DASUVS010000221         8988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1837801, whole genome shotgun sequence.
ACCESSION   DASUVS010000221 DASUVS010000000
VERSION     DASUVS010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8988
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1837801"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /gene="tyrS"
                     /locus_tag="VF182_12360"
     CDS             complement(<1..730)
                     /gene="tyrS"
                     /locus_tag="VF182_12360"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX6437920.1"
                     /translation="MLNAEAQLEIIKRGAVEVLPEEELVRKLRAGRPLKIKAGFDPTA
                     PDLHLGHAVLIQKMKQFQELGHEVVFLIGDFTGMIGDPSGKAETRKQLSREEVLRNAE
                     TYKEQIFKILEPTKTIIEFNHRWMEKLDAVQLIELSAKYTVARMLERDDFKQRFQKHQ
                     PISIHEFLYPLMQGYDSVILKSDIELGGTDQRFNLLVGRELQREYGQEPQVVLTMPLL
                     EGTDGINKMSKSLGNYVGINEIPED"
     gene            complement(735..1529)
                     /locus_tag="VF182_12365"
     CDS             complement(735..1529)
                     /locus_tag="VF182_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX6437921.1"
                     /translation="MRVLILGDVVGRPARRAIRDLVPSLIEREGIDLAIANAENAAGG
                     IGVDVKSAKELLSSGVDVLTSGNHIWKKKEIYQYMDEHSELIRPANYPEGAPGRGWCA
                     WQNDHGSKALIINVQGRVFMPNHVDDPFRSVDAICRERGRHSRVIIVDMHAEATSEKN
                     AMGWHLNGRASIVFGTHTHVQTSDDRILPGGTAYITDVGMCGPLDSVIGMDKETVIKG
                     FLSQLPRQFEVAHENVVLQGIIVDVDDDSGQAREIRRLRIPYQATQ"
     gene            complement(1540..3093)
                     /gene="rny"
                     /locus_tag="VF182_12370"
     CDS             complement(1540..3093)
                     /gene="rny"
                     /locus_tag="VF182_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX6437922.1"
                     /translation="MFSIVIGVLIGVGASLVIAWIRKKQQQADLQIAQNTAARIIDEA
                     KKDANAIKKEAEIYARDSVQQERIEFDKEVRETRRELQAQEKRLVSRDEGLEKRIDSI
                     EKRENEINRREGALKSREKTLDDKSAECDRQIDAARRQLEQVAGLTRDEARKSLIDEM
                     IEEAKHEAAKRIRVIEEEAKAEAARKGQKIVALAIERLAGDFVAERSVTVMPLPNDEL
                     KGKIIGREGRNIRALEAATGIDLIVDDTPETVVISGHNPIRREIARLSLEKLISDGRI
                     HPGRIEEVVRKSEQEIDEAIREAGQKAIFDVGVHGVHPEIVKLLGRLKYRYSYAQNVL
                     LHSIEAAFICGSMAAELGLNEKQARRAALLHDIGKAVDHEVEGSHAIIGAELARKYGE
                     SPKIVNAIAAHHEDVKAETILAPLVDAADALSGARPGARREMIESYTRRLEELERIVN
                     SFKGVEKSYAVQAGREIRIMVQHDLVSDDDAARMAREIAKKIENEMTYPGQIKVTVIR
                     EMRAVDYAK"
     gene            complement(3142..3744)
                     /locus_tag="VF182_12375"
     CDS             complement(3142..3744)
                     /locus_tag="VF182_12375"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX6437923.1"
                     /translation="MFESKTALRVALLSQRKSLSTEKAESWSRSIQARLLETPLYRSV
                     TAIALYSSIENEADTEQIRDHSLRNGKRVVYPRLLPNRSLEFAEIESPTELVRGKSGI
                     AEPTGRKVLSHYDGIDLLLIVPGVGFDPKGNRLGRGQAWYDRVIRQWGSRATPVGLAY
                     EFQVVDHVPVDSWDEKVLFISTQERFIDCQRVPEQTKQVC"
     gene            complement(4465..4713)
                     /locus_tag="VF182_12380"
     CDS             complement(4465..4713)
                     /locus_tag="VF182_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437924.1"
                     /translation="MDKLKIMALDAIEQLEVKINRLIEQHEKAKKEKELMEKKLQQKE
                     SEWHHLKGQIRQYERERLELREKLDKIIGQFSTLDLSE"
     gene            complement(4808..5239)
                     /locus_tag="VF182_12385"
     CDS             complement(4808..5239)
                     /locus_tag="VF182_12385"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6437925.1"
                     /translation="MLAAKQVLCLIFLLSTVACGRNERMDTLYAQRCLSCHGQAGNGD
                     GPLAQSLPVRPPAFRDTVARRTNSQIRRAIAEGQRTMPAFSPALRQSEISDMLQMVRF
                     LSLEGRETRWWEKFDTLVVAHCSLPWETVLGYDDPVEDSKR"
     gene            complement(5355..6428)
                     /gene="modC"
                     /locus_tag="VF182_12390"
     CDS             complement(5355..6428)
                     /gene="modC"
                     /locus_tag="VF182_12390"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HEX6437926.1"
                     /translation="MKLRCRIPLANFELDVDAAFESRITAIFGPSGSGKTTLLDTIAG
                     LRPMSYGEIEIGGKVLFSSARRINLPARQRGIGYVPQEGALFPHLSVRKNMLFGAERT
                     GAVNSAEALRMEHVLEILEIPHLLARAVTQLSGGEAQRVALARAVLSRPQLLLLDEPL
                     AALDVGLKERILPYLARVRDEFGIPMIYVTHNLTEVLTLADWVLMIREGRLITQGVPR
                     ETLRLSPSILQIPGEQLENVFTVVFVESDAKTGKSKVRLQSGEELFVPYLPQPANRTL
                     QIRIAADDIMVATNKPEGISASNVLPGTIRTIDLLDGQAILTILVGEEFYVRLTASAV
                     RRLDLCQDSRVFLIMKTHSFRIL"
     gene            complement(6428..7102)
                     /gene="modB"
                     /locus_tag="VF182_12395"
     CDS             complement(6428..7102)
                     /gene="modB"
                     /locus_tag="VF182_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEX6437927.1"
                     /translation="MIFSFETWQITLFSLAVGLASTLLILPFGIALAWLLARKEWPLK
                     SVLETVVLLPLVMPPVSTGLVLLKTFGRRSPLGQWLYDRGIEIVFNWKGVLIAMAVMS
                     FPLLVRSVRTSFADVNVRLEQIAATLGASPLKIFFVVTIPLAYRGIVAGALLAFSRAL
                     GEFGATILLAGNIPGKTQTLSLAIYNFVQLGKDPEAYVLLGITVALAYLFVWSSEWLL
                     RTGKRV"
     gene            complement(7188..7964)
                     /gene="modA"
                     /locus_tag="VF182_12400"
     CDS             complement(7188..7964)
                     /gene="modA"
                     /locus_tag="VF182_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6437928.1"
                     /translation="MKTRTDISALVILVYATVLICFTPTRARAEEILVSAAASLTDVL
                     QEISGGYRSMSKHTVKFNFGPSSGLARQIDEGAPADMFFSADLPQMEKLDKSGRLEPG
                     TRKNLLSNQLVVIVPADSKLAISSAKDLLNTAIKRVALAEPSSVPVGLYSKKYLTDEG
                     LWNKVEAKVVPVQDVRATLASVESGNVEAGFVYKTDAAVSKKVKIVYEVPVDKGPKIT
                     YTVAIVKESKRKGVARDFMNYVQSSAAKTAFKKYGFVVLD"
     gene            complement(7966..>8988)
                     /locus_tag="VF182_12405"
     CDS             complement(7966..>8988)
                     /locus_tag="VF182_12405"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX6437929.1"
                     /translation="NQYSPATSVALQLARSLRCRVEDLFSIKQGGDIVEGELLGFLPQ
                     SSAPVRAQVSEVRHRLLVRPLIGTGELTGLGVPADGIIIGSNAGTNRVNVKLLKECEE
                     VRRKVVVAGCDPAMFLAGQHVRKYDRESLVPYLVGSSIALNALKRGEVHVAGIHLAEE
                     KSGRWALPNLQRSVGDMNCMVVTFAHWEEGFIVRQGNPKKIRTITDVVKPTVRIVNRE
                     KGSGARGLLDKQLEAGRIEPSQVKGYGDQVLSHLDVASRIKTGLADVGIGVRSVASIC
                     GLDFVPLERERYDLVIPKSYYEMMAGLRLLLDVIVSKPFRDELEALGGYDTGHTGEMV
                     AEIRSR"
BASE COUNT         2018 a         2572 c         2216 g         2182 t
ORIGIN      
        1 tgtcttccgg aatttcgttt atcccgacgt aattgccgag agacttgctc atcttattaa
       61 taccgtcggt accttcaagc agcggcatcg tcagaacaac ctgtggctcc tgaccatact
      121 cgcgttgcag ttcacgcccg acgagcaaat tgaatctctg gtcagtgcca ccaagttcga
      181 tatcggactt caaaattact gaatcgtagc cctgcatcaa cgggtagaga aattcgtgga
      241 tgctaatcgg ctggtgtttc tgaaaacgct gcttaaaatc atcccgctcc agcattctcg
      301 cgacggtgta ttttgcgctg agctcgatca attgcactgc gtcgagtttt tccatccatc
      361 gatggttgaa ctcaatgatg gtctttgtcg gttccaaaat cttgaagatt tgctctttat
      421 aggtttccgc attccgaagg acctcctcac ggctcagctg tttacgtgtt tccgccttac
      481 ccgacggatc acctatcatg ccggtgaagt ccccgattaa aaaaacgact tcatgtccca
      541 gttcctggaa ctgtttcatt ttttgaatca aaacagcgtg gccaagatgg agatccggcg
      601 cggtggggtc gaagccggct tttatcttta acggtcgacc ggctctcaac ttcctaacca
      661 gctcttcttc aggaaggacc tcgacggccc ctcgcttaat tatttcaagc tgggcttccg
      721 cgtttaacat gtcgtcactg tgtcgcctgg taaggaattc gtaagcgtct aatctcgcgg
      781 gcttgcccag aatcgtcgtc gacatcgaca atgatacctt gcaggacaac attttcgtga
      841 gccacctcga actgccgagg aagctgactt aaaaatccct tgatcactgt ttctttgtcc
      901 atcccgatga ctgaatccag tggaccacac atcccgacat cagtgatata agcagtaccg
      961 cccggcaaaa tccgatcgtc ggaagtctgc acatgagtat gggtcccgaa aacgatggaa
     1021 gctcgcccgt tcagatgcca acccatggcg ttcttttccg aggtcgcctc tgcatgcata
     1081 tcaacaataa tgacccgcga atgacgcccc cgttcccgac aaatcgcatc gacactgcga
     1141 aaaggatcgt ccacatgatt aggcataaag acacgtccct gaacattgat aatgagcgcc
     1201 tttgaaccat gatcattttg ccacgcgcac cagcctcgac caggcgcgcc ttcgggataa
     1261 ttcgcgggcc ggatcagctc cgaatgttca tccatatact gatagatttc ctttttcttc
     1321 cagatgtgat ttcccgaagt gagaacatcg accccagaag acaggagctc ttttgcgctc
     1381 ttcacatcga cccctatgcc accggcggca ttctccgcat tagcaatcgc cagatcgatt
     1441 ccttctcgct cgatcaatga cggaaccaaa tctcgaatgg ctcttctcgc cggccgacca
     1501 accacatccc caagaatcag aacacgcatt ccgaatcctt tacttggcgt agtcgaccgc
     1561 gcgcatttcg cgaataaccg taacttttat ctgtccgggg taggtcattt cattctcaat
     1621 cttcttggcg atctcacgag ccattcgcgc cgcgtcatcg tcggacacaa gatcatgctg
     1681 caccatgatc cggatctctc tcccggcctg aaccgcatag gacttctcca cgcctttaaa
     1741 ggaattgacg atgcgctcga gttcttcaag gcgtcttgta taactctcga tcatctcacg
     1801 acgcgcgccc ggccgggcgc ccgaaagcgc atcggcagca tcaaccaacg gcgccagtat
     1861 cgtttcagcc ttcacatcct cgtggtgggc ggcgatggcg ttgacaatct tcggcgattc
     1921 gccatatttc cgtgccaact cggctcctat aatcgcatgg gatccttcca cctcgtgatc
     1981 caccgcttta ccgatatcgt gcaataacgc tgcacgcctg gcttgcttct cgttcagtcc
     2041 caattcggct gccatggagc cgcaaatgaa agctgcctca atcgagtgaa gcaaaacatt
     2101 ctgcgcataa ctatatcgat atttcagtcg ccccagaagt ttgacgattt cgggatgaac
     2161 gccatgaact ccaacatcaa agattgcctt ctgtccggct tctcgtatag cttcatcgat
     2221 ctcctgttcg gatttccgga ccacctcttc gattctgccc gggtgaatac ggccgtcgga
     2281 aatcagcttc tccagcgaca atcgcgctat ttcccgtcga attgggttgt gtccggatat
     2341 gacaaccgtt tcaggcgtgt cgtccacgat gagatctatc ccagttgccg cctcaagcgc
     2401 gcgaatgttg cgcccttcac ggccgattat ctttcccttc aattcatcgt tcggcagggg
     2461 cataacggtc accgatcgtt ccgcgacaaa gtcgccagcc agtctttcaa tcgctaaggc
     2521 gacaattttt tggccttttc gagccgcttc ggccttggct tcctcttcaa tgacccgtat
     2581 acgtttggct gcctcgtgtt tggcctcctc aatcatttcg tctatcagac ttttcctggc
     2641 ttcatcgcga gtaagcccgg ctacttgttc cagctgtcgc ctggctgcat cgatttggcg
     2701 atcgcactct gccgacttgt cgtcgagagt tttctctcga gatttaagcg caccctcacg
     2761 gcgattaatc tcattctccc ttttttctat agaatcgatc cgtttttcca atccctcgtc
     2821 tctgctgacc aagcgctttt cctgcgcctg gagctcccga cgcgtttcac gcacctcctt
     2881 gtcgaactct atacgttctt gttgaacact gtccctggca taaatctcag cctctttttt
     2941 gattgcgttg gcatctttct tcgcctcgtc aataatccgg gccgcggtgt tctgcgctat
     3001 ctgtaggtcc gcctgctgct gttttttgcg tatccatgcg ataaccaaag atgcaccaac
     3061 gccaatcaat actccgatga caatcgaaaa aatattcgcg tccaataaca gcaccctccc
     3121 ttctggtgat atagtcctag cttagcaaac ctgcttggtc tgctcgggca ctctctgaca
     3181 atcaataaac cgctcctgcg tcgaaatgaa aagaaccttc tcatcccagg aatccaccgg
     3241 cacgtgatca acgacctgaa attcgtaagc cagtcctaca ggcgttgccc gcgaacccca
     3301 ctggcgaatc acgcggtcat accatgcctg accgcgacct aatcgatttc ctttcgggtc
     3361 gaaacctacg ccgggcacaa tgagcaagag gtcaatcccg tcgtaatggc tcaagacctt
     3421 gcggcctgta ggctcggcaa tccccgattt gccgcggacc aattccgtag gggattcaat
     3481 ctcggcaaac tctaaactgc ggtttggaag caaacgtgga tagaccactc tcttgccatt
     3541 gcgcaatgag tgatctctta tctgctcagt atccgcctca ttttctatcg agctataaag
     3601 agcgatggct gttaccgatc ggtatagagg tgtttcgaga agccgtgcct gaatggagcg
     3661 gctccaagac tcggcctttt cggtcgatag cgacttccgc tgcgacagca gcgcaactcg
     3721 caaagctgtt ttgctttcga acaccaatat cccctatggc gtagtccgcg gcggggaaac
     3781 gaggagagac tgtgagagga ggaatgtctc taaagatctg aacgtcggat cgagagggtg
     3841 ggtcctcggg agagtcggcc cccggctaaa ataaccacag gtgtgtactc cgccgtactt
     3901 ggcgctaatt catcaactct attgaacgta aaccggtcca gtcggattcc ccgcgcgcgg
     3961 agatacccca aacacccgag cacccaacac atcagatttt gaaatatctt tgctccccta
     4021 attctcatca tctttcccag ccgccgtctt aataatccgc cgcttgcgcc gttcaacgac
     4081 taaacctgac cataccgctg ctatagcggg cccgtatcca ttggctcacg gtgctccgtc
     4141 gacccacaca attcggcgaa tctaagctcg tcaagcgtcc tcactcaatc tggctgacaa
     4201 ttcttttgat agctggttga ctcgttttaa catcgtttca tgagcttctt ttagccgata
     4261 atactcgtcg gctatattta aagctgttaa catcgcaacg ttcgatttgg ccgccggttt
     4321 cgaggatcga gacagctctt gcatcttgtc attaacgtaa ctggcgactc tcaatatgta
     4381 gctttcttcc gcatcgctgc tgatcgtaaa actttgaccc agaatttcca catttagcga
     4441 tcgcttcata ccgcgtactc aagctcattc cgagaggtcg agagtggaga actgaccgat
     4501 gattttatcg agcttctccc gtaactcgag acgttcacgc tcatattggc ggatctgccc
     4561 cttgaggtgg tgccactcgc tctctttttg ctgaagcttc ttttccatca attctttttc
     4621 ttttttggct ttctcgtgct gctcgatcag ccggttgatc ttcacttcaa gttgttcgat
     4681 tgcgtcgaga gccatgattt tcagtttgtc caaacttcat gtttgagttt agttttaagg
     4741 gtaccccttg tcaagttcat tatcgagaca ctggcttatc gccgcactgc gccgattggc
     4801 tgagtagtca tcgctttgaa tcttcaaccg ggtcatcata accgagcacc gtttcccagg
     4861 gtagactaca gtgagcaacc actagggtat cgaatttttc ccaccaacga gtctcccgac
     4921 cctcgagact gagaaatctt accatttgca gcatgtccga aatttcggat tgacgaagcg
     4981 ccgggctaaa cgccggcatc gtgcgttgcc cttcggcaat tgctctacgt atttggctgt
     5041 tggttctgcg cgcaacggta tccctgaagg caggaggccg aaccggtaac gactgagcca
     5101 acgggccatc cccattccca gcttgaccat gacagctgag gcagcgctgt gcgtaaagcg
     5161 tatccatacg ttcgttgcgc ccgcaggcaa ccgtgcttag caggaatatc agacacagaa
     5221 cttgtttcgc tgcgagcatg tcttatgttt atctaatgac gggtatacgt ggtgaacatg
     5281 aacaagatga ctttagcaaa atcagcggca gagctcgact gttcagaaac gcagagagat
     5341 cagcgaggcc gggcttacaa gattcgaaac gagtgagttt tcataatgag aaaaacgcgg
     5401 ctgtcttgac ataggtccag ccggcgcacc gcggaagcag tgagtcttac atagaattct
     5461 tctccgacga gtattgtcag tatggcttga ccgtcgagca agtcgatggt ccgaatcgtc
     5521 ccaggcaaga cgttgctcgc ggagatacct tcaggtttgt tggtcgccac cataatatcg
     5581 tcggcggcaa tgcgaatctg cagtgtgcga ttcgcgggtt gaggtaggta cggaacaaaa
     5641 agctcttcgc cactttgcag gcggactttg cttttcccgg ttttcgcgtc cgattccaca
     5701 aaaaccacgg taaagacgtt ttccaactgc tctccgggaa tttgtaagat cgaaggagat
     5761 aatctgagcg tctcgcgggg aacaccctga gtaatcagtc gaccttcgcg gatcatcaaa
     5821 acccaatctg ccaaggtgag cacctccgtg agattgtgcg tgacataaat catcgggatg
     5881 ccaaactcgt cccggacgcg ggcgaggtag ggcaggatgc gctccttcaa gccgacgtcg
     5941 agcgcagcca gcggttcgtc gagtaaaagc aactgtgggc gcgataaaac cgcgcgagcc
     6001 agcgctaccc gctgggcttc gccgccagag agttgagtga cggcccgggc caacagatgg
     6061 ggaatttcca gaatttcaag gacatgttcc atgcgcaggg cttcagcact attgacagcg
     6121 ccggttcgtt cggcgccgaa aagcatattt tttcgcacgg aaagatgcgg gaacaaggcg
     6181 ccttcttgag gcacatagcc gatgccacgt tggcgcgccg ggaggttgat cctgcgcgcc
     6241 gaggagaaaa ggactttgcc gccaatctcg atctcgccat agctcatcgg gcgcaatccg
     6301 gcaatggtgt cgagcagggt tgttttcccc gaacccgatg gtccaaagat cgcggtgatg
     6361 cgcgactcga aagcggcatc aacatcgagc tcaaagttgg cgagcggtat gcggcagcgg
     6421 agcttcatta gacccttttt cccgttcgca gcagccattc cgacgaccac acaaacaagt
     6481 acgcgagcgc gaccgtgatg cccagcaaga catacgcttc gggatccttg cccaactgca
     6541 cgaaattgta gatcgccagg gacagggtct gggttttccc gggaatattg ccggccagca
     6601 aaatggttgc gccgaactcg cccagagcgc gcgaaaacgc cagcagcgcg ccggcaacga
     6661 tacccctata agcgagcgga atcgtgacga cgaagaatat cttcaagggc gaagcgccga
     6721 gcgtagcggc gatctgttcg agccggacat tgacatcagc gaatgatgtg cgaacggaac
     6781 ggaccagtag gggaaaagac atcaccgcca tcgcgatgag cacgcctttc cagttaaaca
     6841 cgatttctat gccgcgatca taaagccatt gtccgagcgg gctgcgacga ccgaaagttt
     6901 tcaagagaac cagtcccgtc gataccggcg gcatgaccaa tggcagtagc acgacggttt
     6961 cgagaacgct cttgagcggc cattccttgc gcgccaacag ccatgccagc gcgatgccga
     7021 acggcagaat caacaacgtg ctggccagtc cgacggctag cgaaaaaagc gtgatttgcc
     7081 aggtttcaaa cgagaagatc acgaaacgaa gaaatctccg ttcaatcgcc gggggtcaat
     7141 tctccgcagg cagtttgttg atacttcgca gctctctgag gagtatttca atccaatacg
     7201 acaaagccgt atttcttgaa agcggttttc gcggcggaac tttgaacata gttcatgaag
     7261 tcgcgtgcca cgcccttgcg cttcgattcc ttcacaatcg cgaccgtata agtgatcttg
     7321 ggacccttgt cgacgggcac ttcgtagacg attttcactt tcttggaaac ggcggcgtcg
     7381 gttttgtaca cgaagcccgc ttcgacgttg ccggactcga ccgatgccag agtggcgcgc
     7441 acgtcttgca ccggcacaac cttggcttcg accttgttcc acaagccctc atcagtaaga
     7501 tatttcttgc tgtagaggcc caccggaaca gaggacggct ctgcgagcgc gactcttttg
     7561 atggcggtat tcaataaatc cttcgccgaa gaaatggcga gcttggagtc cgccggcacg
     7621 atgaccacga gctgattgga gagcaagttt ttacgagtgc ccggttcgag gcggccgctt
     7681 ttatcgagtt tctccatctg cggtaaatca gccgagaaaa acatatccgc cggcgcgcct
     7741 tcgtcgatct gccgggccaa gccgctggag ggaccgaagt tgaatttcac cgtatgtttg
     7801 gacattgacc gataaccgcc gctgatttcc tgcaagacgt ctgtgaggct tgccgcggct
     7861 gagacgagaa tttcttccgc acgtgcccga gtcggcgtga aacaaatcaa gacggtggcg
     7921 tatacaagga tgacaagggc gctgatatcg gtgcgtgttt tcatttcaac ggctgcggat
     7981 ttctgcgacc atttccccgg tatgtccggt gtcgtagccg cccaacgcct ctagttcgtc
     8041 ccggaacggc ttactgacga taacatcgag caacagccgc agacccgcca tcatttcgta
     8101 ataggatttc ggaatcacga gatcataccg ttcccgctcc agcggcacga aatccaggcc
     8161 acagatagat gcgacggacc gaacgcctat gccgacgtcg gccaaacccg tcttgattcg
     8221 tgacgcaaca tcgaggtgtg acaagacctg gtcgccgtag cccttaacct ggctgggctc
     8281 gatgcggccg gcctccaact gtttgtccag aagcccgcgc gcacctgaac ctttctcgcg
     8341 attgacgatc ctgacggttg gtttcacaac gtcagtgatg gtgcggatct tttttggatt
     8401 gccctgccgg acgataaagc cttcttccca gtgcgcgaac gtcaccacca tgcagttcat
     8461 atcaccgacg ctgcgctgca gattgggcaa ggcccatcta cctgatttct cctcggcgag
     8521 gtgaatgcct gccacgtgca cctcgccgcg cttgagcgca ttgagggcga tgctgctacc
     8581 cacgagataa ggcaccaagc tttctcgatc gtattttcgg acatgctgac cggccaaaaa
     8641 catcgctgga tcgcagcctg cgacaacaac tttacggcgt acctcttcgc actcttttag
     8701 caatttgacg ttaacccggt tcgtgccggc attgcttccg atgattatac catcggccgg
     8761 tacgccgaga cccgttaatt cgccggtacc tataagcggc cgcaccagaa gtcggtgccg
     8821 aacctcgctc acctgagcgc gcactggggc gctactctgc ggaagaaagc cgagcagttc
     8881 tccttcgacg atgtccccgc cttgtttgat gctgaacaga tcttcgaccc gacaacgaag
     8941 cgatcgtgcg agctggagcg cgaccgaggt agcgggagag tattgatt
//
LOCUS       DASUVS010000222        17843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1619815, whole genome shotgun sequence.
ACCESSION   DASUVS010000222 DASUVS010000000
VERSION     DASUVS010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 17843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17843
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619815"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VF182_12410"
     CDS             <1..447
                     /locus_tag="VF182_12410"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX6437930.1"
                     /translation="GLSRNFPQHKAAFEKNLTAYVADLNAAIARWEAMAAPLKDVKII
                     AYHQDWSYFANRFGMQIIGSIETKPGIEPTPNHLVSLAQRMQQEKAQIIIYGPQSDRF
                     PRQLAQQTGATVVRLQSMAGALPETDTYIKFVDYNLRSLLGAVKKG"
     gene            485..1162
                     /locus_tag="VF182_12415"
     CDS             485..1162
                     /locus_tag="VF182_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6437931.1"
                     /translation="MTKSPLVSLSNARLGYPDTAILHNVDLEIYPGDLIAMAGPNGSG
                     KTTLFRTILGFLPLLAGSLIRNCALHEFGYVPQSAALDPTFPISSGEVVEMGAYGRLR
                     PFQQTPAAERQRVGEVLEEVGLRHLASRSFFSLSGGQKQRILIARALMVRPKILILDE
                     PLSGVDSESQAAITELLLKINREEGKAVFFSSHDLRMVRHVASKVLRVDGGRLAWEES
                     TMTEYPW"
     gene            1156..2025
                     /locus_tag="VF182_12420"
     CDS             1156..2025
                     /locus_tag="VF182_12420"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX6437932.1"
                     /translation="MVSFFDIINPSFILFPALLGSAILAMVCPLVGAHLILRRRVFLG
                     LILPQVAACGVAFTFWIYHEAGAIHGAGGERLLAVAGSLFFTLAGMAVLDHLDRHASG
                     SSEGRLAAAYALASALTILFIVFNPAGELEILNLLKGEVIALSREELRLLAIVYAVVF
                     FAMILFRREFLLSSFDQDLAFLLTGGNAQWGFWLYVLCGLSIAVGVIMAGPLLVFGFM
                     VLPPLAARALARGMGSFFLLSSLFGVGIAILGFYTSVKLDLPLGPTDVTLGCAVIFLI
                     YAVRAGLHKCGSG"
     gene            2162..3334
                     /locus_tag="VF182_12425"
     CDS             2162..3334
                     /locus_tag="VF182_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6437933.1"
                     /translation="MASILINNIGTLVTGKLEDPLRKADSIYIEDGLVRAIGNGLKQK
                     ADQIIDANGITAIPGLIDSHSHPSIGEYTPAQNALTWITNYMHGGVTALISAGELHLP
                     GLPLPPDARTALSVAIVTKRCYDNLRPSGVKVHAGTLLLVPGLAEKDFDEIQALGIKL
                     VKFIFYDYSLLPNGEAERYVKWAKARGIKVKIHSGGVSRSGVSQVAGFDIVKKIQPDI
                     IGHITGGPIPMAEEDMIRIASETECFMEICTSGNYRLALVLARHVKANNLHHRVLIGT
                     DTPGGTGVVPRGILREVAFLSSVGEIEPEKSICMATGNVGRAHGLEIGILEEGRPADL
                     VLLHKITGSVANDALESFAKGDLPGISTVIIDGEVRVAGRSQQTPPPERLAIQSCC"
     gene            3402..4382
                     /locus_tag="VF182_12430"
     CDS             3402..4382
                     /locus_tag="VF182_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6437934.1"
                     /translation="MSTFEFGVGLFPTEPLPQMMQLAKVTEQLGYSHIWVGDSHLIWR
                     EAYVNMAAMALNTATVKLGTGVTNPLTRHPSVVASAYATLEEYAPGRTIVGIGLGDSS
                     VETMGMKPAKLSYFEKTMAQMRQLLDGQEVQLETGKIHLLHPCKNKVPIYIAASGPKM
                     LELSGRIADGIIVLVGVADDYIAHARERIFAGAKAAGRKVENINLVLWVPCAVSDSAP
                     AKDAVKAHVARVVAHPLPYVLDPTEQKVLAEIRKTYDYYHHMDQQANHAEVIPDWLVD
                     KFAIAGTVAECSAQIERIKKSGIQQIAIIPYSPPGGSREETIRGFAKAMS"
     gene            4409..4801
                     /gene="gloA"
                     /locus_tag="VF182_12435"
     CDS             4409..4801
                     /gene="gloA"
                     /locus_tag="VF182_12435"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010873329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEX6437935.1"
                     /translation="MKIVHTMIRVNDLDESLKFYCDGLGMKLLRKRDYESGRFTNVFV
                     GYGSEDTDAVIELTYNWDTHSYDIGNGFGHIALGVPDVYKTCDELRAKGVKIVREAGP
                     MKHGSGANAIAFLEDPNGYKIELTTRAE"
     gene            complement(5082..6080)
                     /locus_tag="VF182_12440"
     CDS             complement(5082..6080)
                     /locus_tag="VF182_12440"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6437936.1"
                     /translation="MALSYNKILLALALVHAIALGLAATRSWSQEKYPSRSIEMIVAF
                     PAGGFADVTGRIFAEEMSKVLNTPIATINKGGATGSLAATSMLSAPTDGYNILVNTLG
                     GMVLAPVTLKDIKYDTNKDFDPVAFITSAPDGVTVRAESPYKNLNDLIEAAKKTPNKL
                     AYGTAGTGVGGHFNSLILSQVTAIKIKHVPYKGGGEYIPALMGGHLDFVIGTAIATLP
                     QESAGKLRTLALMGGNKMKALPSVPTTAELGIKGNYLDSWSGAFVTAGTPKPVIDTLT
                     AAAEKVIKSKEFGARIEKTGGLVQYIKPADFRSMIERDKKTALEIAKKEGLGQGAQ"
     gene            complement(6098..6559)
                     /locus_tag="VF182_12445"
     CDS             complement(6098..6559)
                     /locus_tag="VF182_12445"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX6437937.1"
                     /translation="MKRTRILAAVAGAFFWVTLGVLACYGANQLGRGSVAEPGAGFIF
                     FWSGLVLMTLSLIVLADSLRSSDDTIRRIPKINWPKITLVLFSLLLYAFFLERLGFVL
                     TTFVLMGFLLGCIEGRGWFRSLGIASAAALTSYALFELWLKIRLPKGFFGF"
     gene            complement(6546..8048)
                     /locus_tag="VF182_12450"
     CDS             complement(6546..8048)
                     /locus_tag="VF182_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6437938.1"
                     /translation="MFESLTSLLYGFNIALQPQNLLYCFIGVTMGTLVGVLPGIGPTA
                     TIALLLPATFSLNPISAIIMLSGICYGAMYGGSTTSILLNIPGEASSVVTCFDGYQMA
                     RQGRAGAALGISAFGSFIAGTVSIFGLILLAPTLADFALRFGPPEYFALMVMGLTVVA
                     YLARKSVAKALMMAVVGILLGCIGLDPITATPRFTFGMLELADGIGIAPVVMGLFGIA
                     EVLENVGVLTKTEIFAPKIKGIFPNREDWKRSAGPMARGSLLGFFLGLLPGVGAIVPQ
                     FLSYALEKKLSRYPERFGNGAIEGVASPEACNNAAVGATFIPLLSLGIPSTAMTAILL
                     GALMIYGLTPGPLLIKNSPDLFWGVIASMYIGNLMLLILNLPLIPLWVRVLKIPYSYL
                     SCFIILFCLIGAYSLNNSTTDIYVAIVFSVIGLLMKKFAFEPAPLVLAFVLGPLLETA
                     LRRSLIQSDGSVLIFLQRPISAAFILFSIIVLVVPLFSKMRLGRELSEED"
     gene            complement(8206..8946)
                     /locus_tag="VF182_12455"
     CDS             complement(8206..8946)
                     /locus_tag="VF182_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX6437939.1"
                     /translation="MEWIFDPQAWIAFATLLALEVVLGIDNVIFISILAGKLPGSEQA
                     KARYVGLGLAMVMRIILLFSISWVIGLTAPLFTVFEQEISGRDIILLVGGLFLIAKST
                     HEIHQRLEGGEAHASAAAAASFNSVIIQILLLDIVFSLDSVITAVGMVEDISIMISAV
                     VFAVLFMMAFAGPIGRFVERHPTVKMLALSFLLLIGMTLIAEGFDQHIPKGYIYSAMA
                     FSVFVEMLNLNAKKPKAAPVKLHRRYAE"
     gene            complement(9076..9702)
                     /locus_tag="VF182_12460"
     CDS             complement(9076..9702)
                     /locus_tag="VF182_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX6437940.1"
                     /translation="MDNIAQFALVTMTSLLFIVDPIAVVPAYLVITSDETAGEQRRTA
                     KRACIVMTLLLIFFAATGNLLFRAFAITLPAFRTAGGLILWYVAMDMLRGERRTQEGQ
                     EEVLEGQTKEDVAITPLAIPMLAGPGAISTVIVLSSQAQGLRELGVVYGSVMLTGLIS
                     YWVLRLGEGLLRRLGKTGIRVLTRIMGLILAAVAVQFVLSGIQEAFGI"
     gene            9894..10259
                     /locus_tag="VF182_12465"
     CDS             9894..10259
                     /locus_tag="VF182_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEX6437941.1"
                     /translation="MARVIAAPTVIPAAGQPPKIIEEFIGRVNSATSKLSIAKMTSPS
                     GWREQGQTPEFDEFTVVLRGQLQVETRDAVHQISAGQAIIVNAGEWVRYSTPFVSGAE
                     YIAVCLPAFTPETVHRDDR"
     gene            complement(10353..11285)
                     /locus_tag="VF182_12470"
     CDS             complement(10353..11285)
                     /locus_tag="VF182_12470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX6437942.1"
                     /translation="MSALKIFSIMITWVVLLHHDASSITIATGSQEGTYYKIAQDLKQ
                     LAEKEGIPIEIISTNGSFDNINLLGAGKVDLAILQLDVLKFVSEIMQKEANFDVFEQA
                     KVVLNLYLEEIHIITRNEGLLSIYQLEGKKVAVGPQRSGSALSAEVLLAGYNLRVQAV
                     FEGPNDALEKLKSGELDALIFVGGAPVPAFEKLDDSFHLVRVPANSTLEQIYKKQKIE
                     RSVYPWAGDSETYAVPSVIMTRARDDREYVTTLQKLLIAILLNKETLDSTGHPKWKSS
                     FIRSIVGNAGYRPAVDVLQIFDVLDEGGYRIIKK"
     gene            complement(11396..11881)
                     /locus_tag="VF182_12475"
     CDS             complement(11396..11881)
                     /locus_tag="VF182_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEX6437943.1"
                     /translation="MDTVQGYYLLATLLVLAGLAGLVLPGLPGSPLIFAGLFVAAWAE
                     DYTYVGAGTLIVLAVLAVVTYAVDFWATMFGAKRFGASKRAIVGALIGAIVGLFLGLP
                     GVIFGPFIGAVIGELSAQRGLQDAARAGIGATIGLAIGVAVKMAVAFSMIGIFIFARF
                     F"
     gene            12237..13397
                     /locus_tag="VF182_12480"
     CDS             12237..13397
                     /locus_tag="VF182_12480"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437944.1"
                     /translation="MTHPLADNLSSLRVDLVGSLLRPAKLKQAFSKWASGEISEEELQ
                     KFQDEAIRQVIAKQEAHHLPLVVDGEFRRTNFMESFSVVAGVEEWQSGVKTYHNILAR
                     EDSETSKTAKGQDPILLNRKRVTQRLRLVRNRLLEDYRFAQSLTLRPVKVSLIGPDRI
                     TQCYDSETSRSVYPSIDDFVADVVTVERDMIGQLAEAGCRYVGIDAPGYTAYVDGPSL
                     AAMRERGEDPEQNMERSIRADNQIIADFSDLMFGIHLCRGNRQSMWHREGSYDAIAEK
                     LFNGLNHHRLLLEYDTERAGSFEPLRLVPKGKTVVLGLITTKVGRVETVDELRRRIDA
                     AAGYIPIEQLALSPQCGFASSIMGNLLSEDEQFRKLDVMLETAAKVWGTAAA"
     gene            complement(13629..14354)
                     /locus_tag="VF182_12485"
     CDS             complement(13629..14354)
                     /locus_tag="VF182_12485"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437945.1"
                     /translation="MYGWRARIGHVAPSRGDTLVYEFYKMFPEGFMMLNTTGTVRQLV
                     DNDFEKQLQRIEEAVQDLVDNNCDSIIFSGSPLFTRLPFGTDREMGRKLTEKFGVPVA
                     AGLTAEVEALKAMEIKKLVVGTPYEEEINQRLKRHLEASGFEVLQIAGYGVRKNAQLT
                     DLPVHASYKIAKRLYAKGRNADGIFIPCPRWPTITDVALLEEEIGKPVITSSLACIWH
                     ALKLIDIKENVTGYGRLMASLGN"
     gene            14583..15152
                     /gene="pal"
                     /locus_tag="VF182_12490"
     CDS             14583..15152
                     /gene="pal"
                     /locus_tag="VF182_12490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX6437946.1"
                     /translation="MSVRSWGLLAMISLLGCGNPVNPPEPKWTAENPNASSSAKSTAA
                     SAQKPVAENKADKSGGGSLDDLQKGGTAATGPLKDVFFAFDRSDLSPDARSILKANSD
                     WLKANPAAQVQIEGHCDERGTVEYNLALGSKRAQASKDYLETLGIPANRLSMISYGEE
                     IPVCRENNDSCWEKNRRARFIVTSGRPAS"
     gene            15326..16945
                     /locus_tag="VF182_12495"
     CDS             15326..16945
                     /locus_tag="VF182_12495"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX6437947.1"
                     /translation="MDKVLNQILDLADHGTVEQRCASLLVLGALKLQSTETIKTVGPI
                     LEHANPILKDYALRYFEQVQAKAGIPLFVKLLDDPDRDVQERAVRLLMQSGAATVDLL
                     VKNSSGASRHWQLNAARILCAIQGSNARQALLEMLRAGSDDFNKSVCDLMTPAIREMD
                     VKEQGLFYGELEEFGKKLDEKQQRPALVSVMRLLGQLGRADARRWLFRFIGPEHHSVL
                     RSHALVALLRCLREQDLRKDEYAKLFPLLEEAEMTELTRLALELLDKHELPDDSRSLL
                     ARLLESGHPEVQKFALRKMAGFGTPGTVRTLIEQLGDTDYRRRDVAASSLRKMPEART
                     ALIKELLTCEEPSKAWSIAELLASVEGKWRQDTVDALWDRLQAAISAEDRIQTAFLHV
                     MKKADIDQTYDQLARQAARLIKAKKYKEGLAFLTPLKEFPGFKPEHKFQIALAQLKLH
                     SHSVASHRQHPVLDLFTDLYRNSAFPLYEALKKEKSLAPEEYFALGFSLVERSGQERN
                     LGKDLLDHVAAKSPRTKIGKSAKNKLKLLNW"
     gene            17253..>17843
                     /locus_tag="VF182_12500"
     CDS             17253..>17843
                     /locus_tag="VF182_12500"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437948.1"
                     /translation="MKNHRGLMCVAVVVSVVNWSAAVSAQLTKVAIGYSGISADQLVI
                     WVAKDAGIFAKNNIDAQTVYFSGGTTSVTAMISGDTPLIQASGPGIVSAGLAGADPVY
                     VVGGITTLDYWLMTQPEIKTPEQLKGGTIAVARFGGAADFVARYALARLGLTPGKDVT
                     IVQTGSTPERLAAMESRRVSGSTLVPPAMFTAQKKGF"
BASE COUNT         4122 a         4791 c         4952 g         3978 t
ORIGIN      
        1 ggtttgtcgc gaaatttccc ccaacataaa gctgccttcg agaaaaatct gactgcatat
       61 gtcgccgacc taaatgcagc gatcgcgcgc tgggaggcaa tggcggcgcc gctcaaggac
      121 gtgaagatca ttgcctacca ccaagattgg agttatttcg ccaatcgttt tgggatgcag
      181 attatcggca gtatcgagac caagccaggc atcgagccaa cgccgaatca tctggtctca
      241 ttagcccaga gaatgcagca ggaaaaagcg cagatcatca tttatgggcc tcagagcgac
      301 cgattcccgc gccaattagc acaacagacc ggtgccacag tcgttaggct gcagagtatg
      361 gccggcgcat tgcccgaaac cgacacgtac atcaaattcg tcgattacaa tcttaggagc
      421 ctcctcggcg ctgtaaagaa gggatagagt tttatggatg cacccggcag caggcggtca
      481 ctccgtgact aaatcgcccc ttgtgagcct cagcaacgcg cgattggggt atcccgatac
      541 agcgattttg cacaacgtcg atctggaaat ttatccggga gatttaatcg ccatggcagg
      601 gcccaatgga tcgggcaaga cgacgctctt tcgaacgatt ctcgggtttc tacctttgtt
      661 ggccggttcg ttaatccgga attgcgcgct gcacgagttt ggctatgttc cgcagagcgc
      721 ggctctcgat ccgacatttc caataagttc tggcgaagtg gttgagatgg gcgcctacgg
      781 acgactcaga ccgtttcaac aaacccctgc cgccgagcgt cagcgcgttg gtgaagtcct
      841 ggaagaagtc ggacttcggc atttggcgag ccgctctttt ttctcgttat cgggcgggca
      901 aaagcagcgg attctgatcg cccgcgcgct aatggtgcgg ccaaagatcc ttattctaga
      961 cgaacctctg tccggcgttg acagcgaatc gcaagcggcg atcaccgagc tattactcaa
     1021 gatcaatcgc gaggaaggca aggcggtatt tttctccagc catgatttac gaatggtgcg
     1081 tcacgtcgcg agcaaggtat tgcgtgtcga cggtggccgg ctcgcctggg aggaatcgac
     1141 aatgacggaa tatccgtggt gagttttttt gatatcatca acccgtcgtt cattctgttt
     1201 cccgccttat tgggcagtgc cattctcgct atggtttgtc ctctggtggg cgcgcatttg
     1261 attttgcggc gtcgagtttt tctcgggctt attttgccgc aggtggcggc ctgcggggtg
     1321 gcctttacct tttggatcta tcacgaggcc ggcgcaattc acggcgcagg cggtgaaagg
     1381 ctgctcgcag tggctggctc tttgtttttt actctggccg gcatggcggt tttggatcat
     1441 ctcgaccgtc atgctagcgg gtcttcggaa ggtcgcctcg cggcagccta tgcgctggca
     1501 tcggcgttga cgattctatt catcgtcttc aatccggccg gtgagctgga aatcctcaat
     1561 cttctcaaag gcgaagtgat cgctttatca agggaagagc tcaggctcct agctatcgtc
     1621 tacgccgttg tgtttttcgc gatgattctg ttccggagag aattcctctt gagctccttc
     1681 gaccaggatc tggcgttcct gttgaccggc ggaaatgcgc agtggggttt ttggctctac
     1741 gttctgtgcg gcctcagcat cgcagtcggg gtgatcatgg cggggccgct gctggtgttt
     1801 ggtttcatgg tgttgccgcc gctggctgca cgcgcgctgg cgcgcggcat gggttccttc
     1861 tttttattat catcactatt tggcgtcggc atcgcgatcc taggttttta tacttcggtc
     1921 aagttggatc tgccgctcgg acccaccgat gtcactctgg gctgcgcagt gattttcttg
     1981 atctatgctg tgcgggctgg gctgcacaaa tgcggctctg gctaatgctc aagtgcttga
     2041 ttcatcgttg gcagtgacaa accgatttct accgtcgtag aattctgcgc aagtgttgct
     2101 cgccgctgat cgcatctcac cctttacagt cctagaagct ttgatatagt tccgcacaag
     2161 tatggcttca attctcatca acaacatcgg cacactggtt accggcaaat tggaagatcc
     2221 cttgcgcaag gccgattcca tttatatcga agacggttta gttcgcgcca tcggcaacgg
     2281 cttgaagcag aaggcggatc aaatcatcga tgccaacggc atcaccgcga ttccagggtt
     2341 gattgattcg cattcgcatc catctatcgg tgaatacacc ccggcgcaaa atgcgctcac
     2401 atggataacc aactacatgc atggcggcgt aaccgcattg atctccgccg gcgaattgca
     2461 tcttcccggc ctgccgctgc cgccggatgc tcgtaccgct ttgtcggtgg cgatcgtgac
     2521 caaacgctgc tacgacaatc tccgcccgtc gggggtcaaa gtgcacgccg gcacattgtt
     2581 attggttccc gggctggcgg aaaaagattt cgacgaaatc caggcgttgg gaatcaagct
     2641 ggtgaaattc atcttttatg attacagctt gttgcccaat ggcgaagcgg aacgttatgt
     2701 gaagtgggcg aaagcccggg gcatcaaggt aaagattcat tccggcggcg tttcgcgatc
     2761 cggcgtcagt caggtggcag gattcgatat cgtcaagaaa atccaacccg acattatcgg
     2821 tcatatcaca ggcggtccga ttccgatggc ggaagaagat atgatccgca tcgctagcga
     2881 gaccgagtgc tttatggaga tttgcacgtc gggaaattat cgcctggcgt tggttcttgc
     2941 gcgtcatgtg aaagccaaca acttgcatca tcgtgtgctc atcggcaccg acactccggg
     3001 cggcaccggc gttgtgccgc gcggcatctt gcgtgaggtc gcctttctct cttccgtcgg
     3061 tgaaatcgaa cccgagaaat cgatttgcat ggcgaccggc aacgtcggcc gagcacacgg
     3121 tttggaaatt ggtattctcg aagaaggacg accggctgat ctggtcctgc tacacaaaat
     3181 caccggctcc gtcgccaacg acgccctgga atcctttgcc aagggtgatc ttccgggtat
     3241 ctcgacagtg atcatcgacg gtgaagtccg agtcgccggc cgcagccagc aaacgccgcc
     3301 gcccgagcgg ctggcgattc aatcctgttg ttgaaattct ccgagtttgt tcgagacaag
     3361 ctgcttgatg aacttgcaca gtggcttagg aggaacccgt tgtgagtact tttgaatttg
     3421 gcgtagggct ttttcccacc gaaccgctgc cgcaaatgat gcaactggcc aaggtgactg
     3481 aacagttggg ttatagccac atctgggtgg gcgattccca tctgatctgg cgtgaagcct
     3541 acgtcaacat ggcggcgatg gcgctcaata ccgccacggt caagctcggc accggcgtca
     3601 ccaacccact cacgcgccat ccgtctgtgg tggccagcgc ttatgcgact ctggaggaat
     3661 acgcgccggg tcgcactatc gttggcatag gattgggcga tagttcggtc gagacgatgg
     3721 gcatgaagcc ggcgaagctc tcttattttg agaaaactat ggcgcagatg cgccagctat
     3781 tggacggcca ggaggtccaa ttggagaccg gcaagatcca tttattgcat ccctgcaaaa
     3841 acaaagttcc catttacatc gcggcgagcg ggccgaagat gctagaactc tccggccgca
     3901 tcgccgacgg gattatcgtt ttagtcggcg tggctgacga ctatatcgcc catgcgagag
     3961 agaggatttt cgccggcgcc aaagcggcgg gaaggaaagt cgaaaacatc aacttggttc
     4021 tctgggttcc ttgcgcggtg tcagatagcg cgccggccaa ggacgcggtc aaggctcatg
     4081 tcgcccgggt tgtggcgcat ccgcttccct acgtgctcga tcctaccgag caaaaagtgc
     4141 tggcagaaat ccgcaagacc tacgactact atcatcacat ggatcagcaa gcgaaccatg
     4201 ccgaggtgat accggattgg ctggtagaca agttcgcgat tgccggaacc gtcgccgagt
     4261 gcagcgcgca gatcgaacgg attaagaaat ccggcataca gcagattgcg atcattccct
     4321 acagcccacc aggcgggagc agggaggaga caatccgcgg attcgccaag gcgatgagct
     4381 aatcaggcgc gatatataag gaggaacgat gaaaattgtt catacgatga tccgcgtgaa
     4441 tgatctcgac gagagtttga agttttattg cgacgggctc ggcatgaagc ttttgaggaa
     4501 aagagactat gagtcgggtc gatttaccaa cgtttttgtc gggtacggtt ccgaagatac
     4561 cgacgccgtc atcgagctca cctataattg ggatacgcat tcgtacgata tcggcaacgg
     4621 ctttggccac atcgccctcg gtgtgcccga tgtttacaaa acctgcgacg agcttcgcgc
     4681 caaaggggtg aagatcgtgc gcgaggcggg cccgatgaaa cacgggagcg gcgcgaatgc
     4741 catcgccttc ctcgaggacc ccaacggtta caaaatcgaa ctgacgacgc gcgcggaata
     4801 gtgatttatc gttctacgtt tactccttgc acggccggat attttgatgt gagtctccgc
     4861 gctgtcactt gactgcggag aatctttagc ttgtttcatg ttttctcatg acagccaccg
     4921 tgcgcattaa tcgatgtgca catgatcttc cttgtgcaga gttcttggcg tcctaaatac
     4981 gtcatcgatg ttggcaaatt gcctctttca gcccagtggg tagcccgata aggacttgtt
     5041 catcctgatt cacaaagctg ttaatagtga gattgtcttc ctcactgtgc cccctgaccc
     5101 agtccttctt tcttggcgat ctccagcgcg gtcttcttgt cacgctcgat catcgagcga
     5161 aagtcagcgg gtttgatgta ttgaaccagg ccgccggttt tttcgattcg cgcgccaaat
     5221 tcttttgact tgatcacttt ctcggccgcg gctgtaagag tatcgatgac cggcttgggc
     5281 gtgccggcgg tgacgaaggc tccggaccag ctatcgagat aatttccttt gattcccaac
     5341 tcggcggtgg tcgggaccga gggcagcgcc ttcattttgt tgccgcccat gagcgccaag
     5401 gttctgagct tgccggcgct ttcctgcggc agagtcgcga tcgcggtgcc gatgacaaaa
     5461 tccagatggc cacccatgag cgccggaatg tattcgccgc ctcctttgta tggcacatgt
     5521 ttgatcttga tggcggtcac ctgggagagg atcagagaat tgaagtgacc gccgacgccg
     5581 gtgcccgcgg ttccgtaggc caatttgttg ggggtctttt ttgccgcttc aatcaggtca
     5641 ttgaggtttt tatacggcga ttctgcgcgc accgtcactc cgtccggcgc ggatgtgatg
     5701 aaagcgacag gatcaaaatc cttgttggta tcgtatttga tgtctttgag ggtaaccggc
     5761 gccagaacca tgccgcccag ggtattcact aaaatattat atccgtccgt cggcgcgctg
     5821 agcatggaag tggctgccag gctgccggtg gcgccgcctt tattgatggt agcgatcgga
     5881 gtattgagca ccttcgacat ctcttcagcg aaaattctgc cggttacgtc ggcgaaaccg
     5941 ccggcgggaa atgccacgat catttcaatc gatcttgaag gatatttttc ctgcgaccag
     6001 gaccgtgtcg ccgccaaacc gagagcgatc gcgtgcacca aggcgagcgc cagcaggatt
     6061 ttattgtatg acagtgccat ctttttctcc tttttaatca gaaaccgaag aatcctttgg
     6121 gaagcctgat cttgagccac agttcgaaca aagcatagct cgtcagcgcc gccgcgcttg
     6181 ctatgccgag ggagcgaaac caacccctac cctcgatgca gcccagcaaa aaacccataa
     6241 gaacaaacgt tgttaaaaca aagccgagcc tttccaagaa gaaggcgtac aacaggagag
     6301 agaaaagtac cagagtaatc ttcggccaat ttatcttcgg gattcgtcga atggtatcgt
     6361 cggaactccg aagcgaatcg gcaaggacga taagggataa ggtcatcaga accaaccccg
     6421 accagaagaa aataaaaccg gcgccgggtt cagcgacgct ccctcgaccg agttggttcg
     6481 ctccataaca ggccagcact cccagcgtaa cccagaaaaa tgcgccggca accgcggcga
     6541 gtatcctagt cctcttcact gagttcacgc cccaatctca ttttgctgaa cagcggcact
     6601 actaggacaa tgatggaaaa aagaatgaat gccgctgaga tcggccgttg cagaaagatc
     6661 aatacgctgc catccgattg gatcagcgag cgcctcaaag cggtttcgag caaaggaccc
     6721 aagacgaatg cgagcaccag cggggcgggc tcgaaggcga acttcttcat gagaagtcca
     6781 atgacgctga agacgatcgc gacgtagata tcggtggtgc tgttgttgag gctgtaggcg
     6841 ccgattaggc aaaatagaat gataaagcag gaaagataag agtaggggat cttgagtacc
     6901 cgcacccaca atggaattag cggcaggttg aggatcaaca gcatcaggtt gccgatgtac
     6961 atgctcgcga tcactcccca gaacagatcg ggggaattct tgatcaacaa gggtccgggc
     7021 gtgaggccat agatcatcag tgcccccagc aaaatcgcgg tcatcgcagt ggaaggtatg
     7081 cccaagctca gcagcgggat gaaggttgcg ccaaccgcgg cgttgttgca ggcctccggt
     7141 gaagcgactc cttcgatcgc cccgttgcca aatcgttcgg gatagcgcga gagtttcttt
     7201 tccagggcat agcttaaaaa ttggggtacg atcgcgccga ctccgggtaa gagtccgagg
     7261 aaaaatccca acaacgatcc gcgcgccatt ggaccggcgg atcgtttcca atcttcacgg
     7321 ttaggaaaaa tcccctttat tttcggcgca aagatttcgg tcttggtcag cactccgaca
     7381 ttttccagaa cttcggcgat accgaaaagt cccatcacca ccggcgcaat gccgatccca
     7441 tccgcgagtt ccaacatgcc gaatgtgaac cgcggagttg ccgtgatcgg atccagaccg
     7501 atgcatccga gcaggatgcc gacgactgcc atcatcaagg ctttagccac cgattttcgc
     7561 gcgagatagg cgaccactgt caaacccata accatgaggg caaagtattc cggtgggcca
     7621 aatcggagcg cgaaatccgc gagcgtcggc gcgagcagga tgagaccgaa gatactgacc
     7681 gtgccggcga tgaatgagcc gaaggcggaa atccccaggg ccgcgccggc gcggccctga
     7741 cgggccatct gataaccatc gaaacaggta accacggaag aagcttctcc tgggatattc
     7801 agcaagatcg aagtcgtcga gccgccgtac atggcgccgt agcagatgcc cgaaagcatg
     7861 atgatggcgg aaatcgggtt gagcgagaag gtagccggca aaagtagcgc gatggtcgcc
     7921 gtcggaccga ttccgggaag gacgccgacg agagttccca tagtgacgcc gatgaagcaa
     7981 tagagaaggt tttgcggctg cagcgcgatg ttaaagccgt agaggagact cgttagagac
     8041 tcaaacattg gattatgctg agaccctcaa ggggcgggca gaagtaaagt ctatcacaga
     8101 ttcaaccagc gcggctggtt gcaatgtaga cgtgacgacc gtctgctata gctcaggcaa
     8161 aaccggaatc gcagggtctg tagccgtaat ccatagtccc gctccttatt ccgcatagcg
     8221 tcggtgtagc ttcaccggcg ctgctttggg ttttttcgcg ttcagattga gcatctcaac
     8281 aaataccgaa aacgccatag cggagtagat gtaacctttc gggatgtgct gatcgaaacc
     8341 ttcggcgatg agagtcatgc cgatcaatag taaaaagctc agcgccaaca ttttcaccgt
     8401 tggatgccgt tcgacgaagc ggccaatcgg gccggcgaat gccatcatga acaacaccgc
     8461 aaacaccacg gcggaaatca taatgctgat atcctcgacc atgccgactg ccgtgatcac
     8521 cgaatccagg gagaaaacaa tatcgagtag aagtatctga atgatcacac tgttaaaact
     8581 cgccgccgcc gcagcggagg cgtgtgcttc gccgccttcg agccgctggt gaatttcatg
     8641 cgtgctttta gcgatgagaa acaggccgcc gaccagcaag atgatgtcgc gcccggaaat
     8701 ttcttgctcg aaaaccgtaa agagcggcgc cgtcaggccg atgacccagg aaatggaaaa
     8761 aagcaggatg atgcgcatca ccatggccaa accaagacca acataacggg ctttggcttg
     8821 ctcggagccc ggcaatttgc cggcgaggat ggaaataaag attacattat cgatcccaag
     8881 cacgacctct agagcgagca gtgtagcgaa ggcaatccat gcctgcgggt cgaatatcca
     8941 ctccatgctc tctttctcct ctcgtcttcg taatggaacg gctaaagatc tgatgcctgc
     9001 tagagtgcag gttaatcggc atgcgcgaaa gttgcggcgt aacctaaccc gacggactaa
     9061 atggtggccg gaggatcaga tgccgaaggc ctcctggatt ccgctcaaaa caaactgaac
     9121 tgcgactgcc gccaagatca accccatgat tcgagtcaaa actctgatcc cagtcttgcc
     9181 gagccgtcta agtaatccct caccgagtcg taacacccaa tatgaaatca gtcctgttag
     9241 catcactgag ccgtagacca cacctaactc ccgcagacct tgtgcttgag atgaaagtac
     9301 gatgaccgtg gaaattgcgc cgggtccggc gagcatcgga atcgcaagcg gcgtgatcgc
     9361 cacatcttcc ttggtctggc cttcgagcac ttcttcctgc ccctcttggg tgcgccgttc
     9421 gccgcgtaac atgtccatgg caacgtacca aaggattaat ccgccggcgg ttctaaacgc
     9481 gggcagggtg atggcgaagg cgcgaaacaa caagtttccg gtcgcggcga aaaatatcag
     9541 cagtagcgtc attacgatgc aggcccgttt agccgtgcgg cgctgttcgc cggctgtctc
     9601 gtcactagtg atgaccaggt aagccggcac caccgcaatc ggatcgacga taaacagcag
     9661 cgaggtcatc gtaaccaaag caaattgcgc tatgttgtcc atctatcgct catcgctgct
     9721 tctacttacg ccttgctcga gatcactcgc gacgccaaat aactcccggc tttgcctgat
     9781 ttgttcgata tcaccgcgcc agcgcccgga gtgttatcct cgcccggagc aacaggtcgg
     9841 atcgcggtat cgattcaatt gcgctggtag agttttgcat gtacgttttg atggtggcaa
     9901 gagtcattgc ggcaccgacc gtaattccag cggcagggca gccgccgaag atcatcgaag
     9961 agtttatcgg gcgggtgaat tctgcaacgt ccaaactcag catcgccaag atgaccagcc
    10021 cgtcaggatg gcgtgagcaa ggccagacgc cggagttcga cgagttcacc gtcgtcctgc
    10081 gcggtcaact gcaagtggaa acgcgcgatg ccgtgcatca aatttccgcc ggacaagcga
    10141 tcatcgtcaa tgccggtgag tgggtgcgct acagcacgcc ttttgtctcg ggggcggagt
    10201 acatcgctgt ttgtttgcca gctttcacgc ccgaaacggt tcatcgcgac gatcggtgag
    10261 actcctctcc agtcgctacg gacgtttgct gtcagttccg agcggcaagc atcacctcaa
    10321 cccggcattt cgtggccgcg gctggcatgt ctttacttct tgatgatacg gtagccgccc
    10381 tcgtctagaa catcaaagat ttgtaaaaca tcgacggcgg gacggtaacc ggcattacct
    10441 acgattgacc gaataaaaga actcttccat tttggatgcc cggttgagtc cagagtctct
    10501 ttgtttagca gaatcgctat cagcagtttc tggagagttg tgacgtattc gcgatcatca
    10561 cgcgcgcgcg tcatgatgac cgatggaaca gcataggttt cgctatcgcc ggcccatggg
    10621 tagaccgatc gctcgatctt ttgtttttta tagatttgct ccagcgtgct gtttgccggc
    10681 acgcggacta gatgaaaact atcgtctaat ttttcaaagg cgggcacggg cgccccgccg
    10741 acaaaaatca gggcgtcgag ctctccgctt ttaagctttt ccaaagcatc gttcggccct
    10801 tcgaaaacgg cttggacccg tagattataa cctgctaaca acacttcggc gctgagagct
    10861 gagccgctcc gctgcgggcc cacggcgact tttttgcctt cgagctgata aatcgagagc
    10921 aaaccttcat tcctggtgat gatgtgaatc tcttccagat agagattgag gacgaccttt
    10981 gcttgctcga acacgtcgaa gttcgcttcc ttttgcataa tctcggagac gaatttcaac
    11041 acgtccaatt gcagaatagc caaatcaact ttgccggcgc cgaggaggtt gatattgtcg
    11101 aacgatccat tggtggaaat gatctcgatg ggaatccctt ctttttcggc gagctgtttg
    11161 aggtcttgag cgatcttgta gtaggtccct tcctgggaac cggtcgcgat ggtgatagaa
    11221 ctcgcgtcgt gatgtaaaag caccacccag gttatcatta ttgaaaatat ttttagcgca
    11281 ctcattctcc aactctcttt ctctcatcaa tgtcgcgcca acatagtgtc ttggaagttg
    11341 aacgcgcaat agacactgtc ctgattggtt gacttgattg cgatggacgc gagttttaaa
    11401 aaaatcgggc gaagataaag atgccgatca tcgagaatgc cacggccatc ttgacggcaa
    11461 cgccaattgc cagaccgatc gtagcgccga tgccggcgcg agcggcgtcc tgcaggccgc
    11521 gttgagcgga aagctcgccg atcaccgccc cgatgaaggg accgaatata actccgggaa
    11581 gaccaaggaa caggccgacg atggcgccga tcagcgcgcc gacgatggcc cgcttggacg
    11641 cgccgaatct cttggcgccg aacatggttg cccagaagtc gacggcatag gtaacgacgg
    11701 cgagcacggc caggacaatc aacgtgccgg cgccgacgta agtataatct tcagcccagg
    11761 ctgcgacaaa gagccccgca aatatcagcg gtgatcccgg cagccccgga agtacgaggc
    11821 cagccaagcc ggcgaggacc agcagcgtgg caagtaagta gtagccttga acggtgtcca
    11881 tcagggatta agtttgaacg ccgccgtact gatcaagcca tcgatcgtca tagtttaaaa
    11941 ttcaagagcg ctgtcgaatg cccatgctag gtgaaaccct aaccaacacc tagcaattct
    12001 agtcccaatt ggtccggcct ggccgaaata ttcaccagga ggtgaaaact actgtactgg
    12061 cgcaatttag tatatatcgg ttcatatcat tccagcattc tacacggcaa tgccggagag
    12121 tcggtcggtg tctctacgtg caaggtatag gcagtttcaa gagcagctcc tctggcgttg
    12181 ctgcgcgcat ggtagtgaag atgctagaaa taggcgaacc ataaaggaaa ctgaatatga
    12241 cgcatccatt agctgacaat ttgagttcat tgcgggtcga cttggtcggg agcctgctcc
    12301 ggcccgccaa gctcaaacag gcattttcta aatgggctag cggcgagatc tcggaagagg
    12361 agctgcaaaa atttcaagat gaggcgatcc gacaagtcat cgccaagcaa gaggcgcatc
    12421 atctgccgct ggtcgtcgat ggcgagtttc gccggaccaa tttcatggaa agcttctcgg
    12481 ttgtcgctgg agttgaagag tggcagagcg gcgtaaagac gtaccataac attctcgcgc
    12541 gtgaagacag cgaaacgtcg aaaacagcga aaggacaaga tcccattctt ctcaatcgca
    12601 agcgtgtcac acaacggttg cggctggtgc gcaatcggtt gctcgaggat tatcgtttcg
    12661 cgcagagcct gaccctccgg ccggttaagg taagcttgat cggaccggat cgaatcacgc
    12721 agtgctatga cagtgaaact tcgcgttcgg tctatccgtc gattgacgac ttcgttgcgg
    12781 atgtggttac ggtcgaacgc gacatgatcg gtcaattggc cgaagcgggc tgtcgctacg
    12841 tcggcatcga tgctccgggc tacaccgcct atgtggatgg gccgtcgttg gcggccatgc
    12901 gcgagcgcgg cgaagatccg gagcaaaata tggagcgctc gatcagagcc gacaatcaaa
    12961 tcatcgccga tttctccgat ctcatgtttg gaattcactt gtgtcggggc aaccgtcaaa
    13021 gcatgtggca tcgcgaaggc agttacgatg ccattgccga gaagcttttc aatggcctga
    13081 atcaccaccg cctgctgctc gaatacgaca ccgaacgcgc cggcagcttc gagcccctgc
    13141 gcttggttcc gaaaggcaag acggttgttt taggtctcat taccaccaag gtgggccggg
    13201 tcgagaccgt cgatgaactg aggcggcgca tcgacgcggc ggccggctat attccgatcg
    13261 agcagcttgc gttgagtccg caatgtggct tcgcctcaag catcatgggg aatcttctca
    13321 gcgaagacga gcaatttcgc aagctcgatg tgatgctcga gacagcggca aaagtctggg
    13381 gcaccgcggc agcatagcgg tttcctaagc taatccgaca taattaagcc ccccgaggcc
    13441 agcgagccct acagccgtgg gcaatgggac agtgcgttga aggccgcccc gcattcccca
    13501 aagggtgatg tttcgtacgt cgaggcctta cagtcaacgg cgcgttcggg tgcccacggc
    13561 ttccggtctc gccgtcctcg ctcaatgctg acggtggcga ctattgtggg attacggcta
    13621 agccttcctt agtttccaag actcgccatc agccgtccat atccggtgac attttccttg
    13681 atgtcgatga gctttagcgc gtgccagata cacgccaggc tgctggtgat caccggcttg
    13741 ccgatctctt cttcaagcaa tgctacatcg gtaattgtcg gccagcgggg gcatgggata
    13801 aaaatgccgt cggcgttccg gcccttggcg tagagtcgct tggcaatttt gtaagaggca
    13861 tgaaccggaa gatcggtgag ttgggcattt ttccgaacgc cgtaaccggc aatctggagc
    13921 acctcgaatc cggaggcttc cagatgccgc ttcagccttt gattgatctc ttcctcatag
    13981 ggcgtgccga caacgagctt tttgatttcc atcgccttca acgcctcgac ttccgctgtc
    14041 agtccggcgg ccaccggcac gccgaatttc tcggtcagct tgcgccccat ttcgcgatcc
    14101 gtgccgaaag gaagcctggt aaaaaggggt gagccgctaa agatgatcga atcgcaattg
    14161 ttatccacta agtcctgcac cgcctcctca atgcgctgca actgcttctc gaaatcatta
    14221 tccaccaact gccgcacggt tccggtggtg ttcaacatca tgaatccctc cggaaacatt
    14281 ttgtagaatt cataaaccaa tgtgtctccg cgcgaaggag ccacgtggcc gatgcgggcg
    14341 cgccatccgt acataaagag accctcctaa gtaagataca tgctcagcgg ctgaggccgc
    14401 ttgtgtactt tatttcacga tgacagcgaa ttaccagaag cgcggatgac ggattttcaa
    14461 ggctggttac ttacaaacaa ttttgttgac acttctaatg taatgcatca caattcgagc
    14521 cgtttcagct tctcgtccgg cttctcaacc caaataatca cgagctcgaa aggagagtct
    14581 aaatgtctgt tcgcagttgg ggtctattag caatgattag tttgctaggt tgcggaaacc
    14641 cggtaaatcc tccagagccg aagtggacgg ccgagaatcc gaacgcatca tcgtctgcaa
    14701 aatcaaccgc cgcctcggct caaaaaccag ttgctgaaaa caaggctgac aaatccggcg
    14761 gtggtagcct ggacgattta caaaagggcg gcacagctgc tacaggcccg ctgaaagatg
    14821 ttttttttgc tttcgaccgt tccgatttat cgcccgatgc ccgttcgatc ttgaaggcaa
    14881 actcggattg gcttaaagcc aatccagcgg cccaggtgca gatcgagggt cattgtgatg
    14941 agcgcggaac ggtggagtat aatctagctt tgggttccaa acgcgcgcag gcaagcaagg
    15001 attacctcga aaccctcggc attccggcaa atcggctgtc gatgattagt tacggcgaag
    15061 aaatcccggt ttgcagagaa aacaacgatt cgtgttggga aaagaaccgc cgtgctcgat
    15121 tcattgtgac gtccgggcgt cccgcatctt aattaccgcc tagagaactt cgcttaacgg
    15181 ctggctgtca agaattcatc cgatcaaagg cgcttcaact tgacttccgg aggcccaatc
    15241 tcatgcgaga ttgggccttt ttttatctcg cacccgtcaa accgatttct tgacttttga
    15301 ccatggatga cgttcaaccg tcactatgga caaagttcta aaccaaatac ttgacctggc
    15361 cgatcacggt accgttgagc agcgttgtgc ttcgctgctg gtgctgggag ctcttaaatt
    15421 gcagagtacg gaaaccatca agactgtggg cccgatactc gaacatgcca atcccattct
    15481 caaagactat gcgttgcgtt atttcgagca ggtgcaagct aaagctggaa tcccgctgtt
    15541 tgtgaaactt ctcgacgatc cggatcgcga cgtacaagaa cgggctgtgc ggctgctcat
    15601 gcagtcaggc gccgccacgg tcgatctgct ggtcaaaaat tcttcgggcg catcaaggca
    15661 ttggcagctc aatgccgcgc gcattctctg cgccattcag gggtcaaatg cacgccaagc
    15721 attgttggaa atgctccggg cgggaagcga tgatttcaac aaatcagttt gcgatttgat
    15781 gacgccggca atccgcgaga tggatgtcaa agagcagggg ctcttctacg gtgagctcga
    15841 agagtttggc aagaagctcg atgagaagca gcagcgaccg gcgctggttt ccgtcatgcg
    15901 cttgttgggc cagctcggcc gcgccgatgc gcgtcggtgg ctgttcagat ttatcggtcc
    15961 cgaacaccat tcggtgctgc gctctcatgc gctggtggct ttgttgcgct gtctgcgcga
    16021 gcaggacctg cgcaaagacg aatacgccaa attgtttccg ctgctcgaag aagccgagat
    16081 gacggaacta acccgtttgg ccctggagct gctcgataaa cacgagctgc cggacgattc
    16141 cagatctcta ctggcccgct tgttggaaag cgggcatccg gaagtgcaaa aatttgcttt
    16201 gcgcaaaatg gccggcttcg gcacaccggg aaccgtgcgt actctgattg agcagctcgg
    16261 tgacaccgac taccgccggc gcgatgtcgc ggcaagctcc ctgcgcaaga tgccagaggc
    16321 ccggaccgcg cttatcaagg agcttctaac ctgtgaggaa ccgagtaagg cgtggagcat
    16381 cgccgagttg ttggcttcgg tagaaggcaa gtggcgccag gacacagtgg acgcgctctg
    16441 ggacagattg caagcagcca tcagcgccga agatcgaatt cagacggctt tcttgcacgt
    16501 catgaagaaa gccgacattg atcaaaccta cgatcagctt gcacgacagg cggcgcgcct
    16561 gatcaaggct aagaagtaca aggaagggct agcctttcta accccgctca aagaatttcc
    16621 tggattcaag cccgagcaca aatttcaaat cgctttggcg cagctcaaat tgcattctca
    16681 ttcagtggcc agtcaccgac aacacccggt actcgatctg ttcaccgatc tgtaccgtaa
    16741 ctcggcgttt ccgctgtacg aagcattaaa gaaggaaaag agtctcgcgc ccgaggagta
    16801 tttcgcgctg ggctttagtc tggtcgaaag atccggccaa gagcgcaacc ttggaaagga
    16861 tttgttggat cacgtcgccg ccaaatcgcc gcgcaccaaa atcggcaaga gcgcgaagaa
    16921 taaattgaag ctcctcaatt ggtgagactt gcttggcgcc gatcttatgc caacgccgca
    16981 gcatgatgta aaacaccttg gtgacggtga tcgaagggcg agccctccgg agcaaagaca
    17041 aatgggccgg tgccttggag cggcctgcaa accagtttgt ggagcgatcg cctggaaaaa
    17101 tgcgagcggg cataaagcac caaagacgtc attgaagctg ttcgtctagt tgtcttcatt
    17161 cctccagccc atgcgtcgat cgagacggtg tcgaagtctt cactgagcat ggatcattcg
    17221 gaccggcaat ctgtaaaccg cgaggaaaca acatgaaaaa tcatcgaggg ctaatgtgcg
    17281 tggcagtcgt cgtttcggtg gtgaattgga gcgcggcggt ctcggcgcag ctgaccaaag
    17341 tcgctatcgg ttacagcggc atcagcgcgg atcaactcgt tatctgggta gctaaagatg
    17401 ccggcatttt tgccaaaaac aatatcgatg cgcaaaccgt ctatttctcc ggcggcacga
    17461 catcggttac cgcgatgatt tccggggaca ccccgctcat tcaagcgtcg ggacctggaa
    17521 tcgtcagcgc cggtctcgcc ggcgccgacc cggtctacgt cgttggcggc attaccaccc
    17581 tggattactg gctgatgacg cagccggaaa tcaagacgcc ggaacagctt aaaggcggca
    17641 ccattgcagt ggcccgattc ggcggcgcgg ccgatttcgt ggcgcgctac gcgcttgccc
    17701 ggctcggact gactcccggc aaggacgtaa ctatcgtgca aaccggcagc acgccggaac
    17761 gtttggcggc gatggagtcg cgccgggtca gcggcagcac cctggtgccg ccggcgatgt
    17821 ttaccgcgca aaagaagggc ttt
//
LOCUS       DASUVS010000223         7120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_962762, whole genome shotgun sequence.
ACCESSION   DASUVS010000223 DASUVS010000000
VERSION     DASUVS010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962762"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1450)
                     /locus_tag="VF182_12505"
     CDS             complement(215..1450)
                     /locus_tag="VF182_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437949.1"
                     /translation="MNCVEAQNHFADLLDHSRAERLFKVNDHLAACSRCRQELAALAA
                     CQRLVSALPPVEPPLGFTTRIMAEVRDAAGRPNLWRRLFSSIQNLPLQVTAVILINVL
                     AVFIYQKEFSQQPSTTVPPASGLQKQAEADKSAPGSSGAPALESEMKKRDASGAQEAQ
                     PNPSFQSEQPRSPAKPEEQSNIRIGGTDDPGARNTAPPASGHPSRVAPSRPREDSSPA
                     VEDGSVRQEQSLRSGGARMERVPQSAPLHDNDTASQDRTFGGHSRSSAGLEEKKARSS
                     LDALDSGTAVFSDRDLILRLKQTARDDSKIEASSELEHFQAGASPSRTRFKDFDGGRQ
                     RAIQTGQPQTVSAIIDVSQYDGFKKELASLGKIESEMPAPANEIDALPKSSAQLRITV
                     TILPPLPSANTLPTQPSSR"
     gene            complement(1447..2025)
                     /locus_tag="VF182_12510"
     CDS             complement(1447..2025)
                     /locus_tag="VF182_12510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6437950.1"
                     /translation="MLAEEIGDAECVKRVQRGDTQSFEILLRRHQHTTFNLIYRFLGD
                     YDEATETAQEVFLSAYNSIQQFRGDATFSTWLYRIAFNHASTRRKSLNNKLQRQVALD
                     DEVVLVDCGENPESSAERKEIQERVQQALNGLDGDDAQIILLRDLQDISYEDIAQTLD
                     VPVGTVKSRLHRARQALRNILAPYFTCDRKVS"
     gene            complement(2280..3212)
                     /locus_tag="VF182_12515"
     CDS             complement(2280..3212)
                     /locus_tag="VF182_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437951.1"
                     /translation="MIRIAVPDLVSNSYFPAIAAVELGFFKSEGLDASIDLLFPVPKT
                     FAALRDGQLDFVVGSAHATLLAFPDWNGAKLLAAIAQHTYWFLVIRKDLNPQRGDLSI
                     VKGLRIGAAPGVDLSLQRMLVEAGIDPAKNNVQIMPIPGAAGPNVSFGLSAAKALEDG
                     KLDGFWANGMGCEVALRGGVGTMVVDVRRGDGPPSARHYTFPALVTTEKRIQQEPETA
                     AAAIRAIIKAHKALKQDIGLATEVGRKRFPPAEAELIAELIRRDLPYYEPTITEEKIA
                     SMNRFATDIGLLSAPVTYDKVVATQFSHLWKEAV"
     gene            complement(3343..3693)
                     /locus_tag="VF182_12520"
     CDS             complement(3343..3693)
                     /locus_tag="VF182_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437952.1"
                     /translation="MKIVRLYTGSDQKSHFEEIDLNFGGNQPMLTTESRAATSAVFRS
                     APAGLFLDRHPAPRRQFLVTLSGSWEIEASNGVKRVFKTGDVMLADDTTGEGHTSRVL
                     GSEPHVFMTVPLAD"
     gene            complement(3708..4130)
                     /locus_tag="VF182_12525"
     CDS             complement(3708..4130)
                     /locus_tag="VF182_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020158744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX6437953.1"
                     /translation="MKLDLAKANMIVDAALENARKSKIRPLCVAVLDDGGNLKALKRD
                     DGASILRPQIAISKAWGAIGMGESSRHLGNRLKELPAFVGALSAMTGGKVVPVAGGVL
                     IMDGEVILGAAGASGATADEDEACVIAGIQAAGLQFKV"
     gene            complement(4175..4606)
                     /locus_tag="VF182_12530"
     CDS             complement(4175..4606)
                     /locus_tag="VF182_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEX6437954.1"
                     /translation="MVEFAPMDISTAKEFLARHHWGVLATRRKEGSLQMSPVTVGIDP
                     AGCAIISSRETAYKVNNLRRDSRAAVCVFTTEFHGEGWVQINGNAEIISLPEAIETLV
                     YLERQAKGENRDWLSFHERMAREKRVIIRIRIDDTGPKRRG"
     gene            4777..5457
                     /locus_tag="VF182_12535"
     CDS             4777..5457
                     /locus_tag="VF182_12535"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX6437955.1"
                     /translation="MKIPNLPVPEILPIFPLTGVLVLPGTVLPLHIFEPRYRNMVEDA
                     LEADKVFGMIQPFVPQPDNLPQPGAEKESPDLYKIGCAGYIEKYEKSSDGRFFIQLKG
                     INRFRFQKELEMRRGYRRVKALYSEFPDASLPEDWHCEREAILQALTLFGQAHGMQVK
                     PEQAQRFSDLELVNLVSVSLPFHPAEKQALLEAQTLKDRENTLINLLRLGAGQADPDS
                     DTTPRTLN"
     gene            complement(5605..6420)
                     /locus_tag="VF182_12540"
     CDS             complement(5605..6420)
                     /locus_tag="VF182_12540"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6437956.1"
                     /translation="MFAKIRHLAMYTHDLASVTHFYQTIFGMKRMTSGTVDESGKQNA
                     NRGHISDGVIGMAILPRYAGIQSGMDHYGFEVTDIKDFVRRMEQYYPDVKIAKGLEYV
                     PFAGLRSYDPAGTQFDIAQAHVGNVREGYNESGWDQSRWINHISIRSDNPMAVAEFYE
                     KIFELKEAETYRDDGSICMTDGKVKLLLRPRDNSLYRGLREGLDHVGFKVESLKAAAD
                     DLDALTVSSPEAAPRKLTVGLHGRLIENDFRKCCLGQHFISDPDGVLIDLSEN"
     gene            complement(6549..>7120)
                     /locus_tag="VF182_12545"
     CDS             complement(6549..>7120)
                     /locus_tag="VF182_12545"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437957.1"
                     /translation="TATLFVLPKLGVQPDKEMNIVQVGSVPARFAALVNGSVQGALLI
                     PPETVKAKELGFRILSNFADIDIHYQQNGVYTTRSYINKRPDVLRGFAMAYSEGNHYI
                     HTNSEGAQRIMRKYLHGDEKAIKEAYTEVVLKATPKIPYPSKPGIQTLLDFLAKTAPE
                     ASNAKPDDFIDVRFVKELEDNGFYAKLYR"
BASE COUNT         1473 a         1940 c         1996 g         1711 t
ORIGIN      
        1 tcctcctgga gcggtgtttc cgcattgcga tttgttcgat tagacttcgc gcggagcaaa
       61 atgttccgaa cacgctgctg aaaaagcctc atacgcttcg ttgcgctcgt tcgcctcgct
      121 tcaatgtact gaagcgtacg gctcagctcg tcgaacgatc gcgcgctttg cgtctagagc
      181 tctttgagca gcgtgtaatc agggggcgag atttctagcg gctcgaaggc tgagtcggta
      241 gagtgtttgc agaaggcagc ggcgggagaa tggttacggt gatccgaagt tgggccgatg
      301 attttgggag agcatcgatc tcgtttgccg gcgcgggcat ttcgctctcg atcttcccca
      361 aactggcgag ctctttcttg aatccatcat attggctgac gtcgatgata gcggagacgg
      421 tttgtggctg acccgtttga attgcccgct gccggccgcc gtcgaaatct ttgaatcttg
      481 ttcgtgacgg tgatgctccc gcctgaaaat gctccaactc cgatgatgct tctatcttac
      541 tgtcatcccg tgccgtttgc ttcagacgga ggatcaagtc gcgatcggag aaaactgcag
      601 taccggaatc aagggcatcc aacgaggagc gcgctttttt ttcttccaaa cccgcagaag
      661 agcgcgaatg gcctccgaag gtgcggtcct gtgaggcagt atcattgtcg tgaagcggag
      721 cggattgtgg aactcgttcc atccgtgctc ctccagatcg taacgactgc tcctgtcgaa
      781 ctgagccgtc ttcaactgcg ggcgacgagt cctccctcgg ccgggaaggc gcgacacggg
      841 atgggtgacc agaggccggc ggcgcagtat tgcgagcgcc agggtcgtcg gtaccgccta
      901 ttctaatatt gctctgttct tctggtttgg cgggcgaccg cggctgttct gattggaacg
      961 aaggattcgg ttgtgcctct tgcgcccccg atgcgtcgcg ctttttcatc tcggattcaa
     1021 gggcaggcgc tccgcttgaa cccggcgccg atttgtctgc ttcagcttgt ttttgaagac
     1081 cgctcgcagg cggaacggta gtggatggct gctggctgaa ctccttctga taaataaaaa
     1141 cggccagaac gttgatcaga atcaccgccg tcacctggag cggaaggttc tgaatggatg
     1201 aaaacaaccg tcgccacagg ttgggccggc cagcggcgtc tcgaacttcg gccatgatcc
     1261 gggttgtgaa gcccagtggc ggctcgactg gcggcaaagc agacaccagt cgttggcatg
     1321 cagcaagcgc agccaactct tgccggcacc gcgagcaggc ggcgagatgg tcgtttactt
     1381 tgaataaccg ttcagcgcgg ctgtgatcca acaaatcggc aaagtggttc tgtgcctcta
     1441 cgcagttcat gatactttcc gatcgcaagt aaagtagggc gccaggatat ttctcaacgc
     1501 ctgtcgggcg cggtgcagcc gcgatttcac ggtgcccacg ggaacatcaa gcgtctgggc
     1561 gatgtcctcg taggaaatat cttgcagatc gcggagcaga atgatttggg catcgtcacc
     1621 atcgagaccg ttaagcgctt gttgtacgcg ttcctggatt tccttccgtt ccgcgctgct
     1681 ctcaggattc tcgccgcagt cgaccagcac cacctcgtca tcaagcgcaa cctgtcgctg
     1741 tagtttgttg ttcaagcttt tgcggcgggt gctggcatgg ttaaaagcga tccgatagag
     1801 ccaagtggaa aaagtcgcgt cgccgcgaaa ctgctgaatc gaattgtaag ccgagaggaa
     1861 tacttcttgg gccgtctcgg tcgcttcatc gtagtctccc aaaaaccgat atatcaggtt
     1921 gaaggtcgtg tgctgatgcc gccgcaacaa aatctcgaag gattgagtgt cgccccgttg
     1981 aacgcgtttg acgcattccg cgtcacctat ttcctcagcg agcatggacc tgttctctat
     2041 tagacggccg tgttgggaaa aaagttcccg tcgctttaaa tcggcaacta gacgtggctt
     2101 tttgggaagt atcaggacat aacagcgaaa attacccgag gccgtcaccc gcgaatgcct
     2161 gaatcggggg tccagtccgg tctcgcctgg attcccgata gaaacattcg ggaatgacgg
     2221 attttagcag atctcggaat aactcgttac gcggcacaat gcgaagacta atcgcctgat
     2281 tagactgctt ctttccagag atggctgaac tgggtcgcga ccactttgtc gtaagtcacc
     2341 ggagccgaca gcagtccgat gtcggtggcg aagcgattca tgctggctat tttttcttcg
     2401 gtaatagtcg gctcgtaata gggaagatcg cgccggatca actccgcgat gagttcggct
     2461 tccgcgggag gaaagcgttt tctaccgacc tctgtggcca aaccgatgtc ctgtttgagc
     2521 gctttgtgcg ccttgatgat ggcgcggatc gccgcggcag cggtttcggg ttcctgttgg
     2581 attctttttt cggtggtcac caatgccgga aacgtatagt ggcgcgccga aggagggccg
     2641 tcaccgcgac gcacatcgac aaccatcgtt ccgacgccgc ctcgtaacgc gacttcgcat
     2701 cccatgccgt tggcccagaa tccgtcaagc ttgccgtctt ccagggcctt tgccgcagag
     2761 agcccgaaag aaacgttggg tccggcggcg cccggaatcg gcatgatttg cacattgttt
     2821 tttgctggat cgatgccggc ctcgaccagc attcgttgca aactcaagtc gacgcccgga
     2881 gccgcgccga tgcgcagccc tttgacgatg ctcaaatcac cgcgctgggg attgagatcc
     2941 ttgcgaatca ccagaaacca gtaggtatgc tgggcgatcg cggccagtaa tttcgcgccg
     3001 ttccaatccg gaaaggccaa tagcgtggca tgtgccgagc cgacgacaaa atcgagctgc
     3061 ccgtcgcgca atgctgcgaa ggttttcggc accgggaaca ataaatcgat gctggcgtcg
     3121 agaccttcag atttgaaaaa gcctagctcg accgccgcga tggccgggaa ataagagttc
     3181 gatactaaat cagggacggc gattcggatc atgacttacc tcaaaaattc taatccatgg
     3241 ctctctaacg ttgaaccggt gggtgattga acggttgaac cgattttcgt tcaacgttca
     3301 aaggttcaac gtcagtcatt aattaaattt ggctcagcgt tcttaatccg ccagcggcac
     3361 agtcataaaa acatggggct cactgcccag cacgcgcgat gtatgaccct cgccggtcgt
     3421 gtcatcggct agcatgacat caccggtttt gaagacccgc ttgacgccgt tgctggcttc
     3481 gatttcccag gagccggaca gcgtcaccag gaattgccgc cgcggcgccg ggtggcgatc
     3541 gagaaaaagc ccggcgggcg cggagcgaaa gaccgcactg gttgcggctc gagattcggt
     3601 cgtgagcatc ggttgattgc cgccgaagtt gaggtcgatt tcttcgaaat gcgacttctg
     3661 atcgctaccg gtatacagcc tgacgatttt cattatgttc tcctgcatta aactttgaat
     3721 tgcagcccgg ctgcttggat gccggcaatg acacacgcct cgtcttcatc cgccgtcgca
     3781 ccgctggcgc ccgccgcgcc gagaatcact tcgccatcca tgatcaacac gccgcctgcg
     3841 accggaacga ccttgccgcc agtcatggct gatagggcgc ccacaaaagc cggaagctct
     3901 ttcagccgat tgcccaggtg gcggctcgat tctcccatgc cgattgcgcc ccaggctttg
     3961 ctgatcgcga tttgcggtcg caaaatgctg gcgccgtcgt cgcgcttgag cgcctttaag
     4021 ttgccgccgt cgtcgagaac tgcaacgcag agcggtctga ttttcgactt tcgcgcattc
     4081 tccagcgccg catcgacgat catgtttgct ttagccagat cgagtttcat aaatctccat
     4141 gtcagtagaa gacgttagta atttgcgcgc taatctaacc gcggcgtttg gggccggtgt
     4201 cgtcgatgcg gatgcgaatg atgacccgtt tctcacgcgc cattcgttcg tggaacgaca
     4261 gccaatcccg gttctcgcct ttggcttgcc gttctaagta caccaatgtc tcgatggcct
     4321 cggggagcga tatgatttca gcgttaccgt tgatttgcac ccaaccttcg ccgtggaact
     4381 cggtggtgaa gacacacacc gccgcgcgcg aatcgcgccg cagattgttg accttgtacg
     4441 cggtttcgcg gctgctgatg atggcgcatc cggcaggatc gatgccgacc gttaccgggg
     4501 acatctgcaa tgagccttct tttctccggg tcgctagcac gccccaatga tgccgcgcca
     4561 agaactcctt cgcggtcgag atatccatcg gcgcaaattc aaccatccta ttcctatagg
     4621 ctgtagctga acttttgaca agctggaaag ccgagcgggc attgcgacag ccgattgttc
     4681 ctgagttgcc gttgccactg cccgggtatt gtccaccttg accgttcctg cgccataacc
     4741 tataaacaag agggtgtcga gagcaattct gtggtaatga aaattccaaa tcttccggtg
     4801 ccggaaatac tgccgatctt tccgttgact ggagttttgg ttttaccggg aacggtgctg
     4861 cccttgcaca tctttgagcc gcgttatcgc aacatggtgg aagacgctct cgaggccgac
     4921 aaggtctttg gcatgattca accgttcgtg ccgcaacctg ataatctgcc gcagcccggc
     4981 gccgaaaagg aatcacccga tctgtacaag atcgggtgcg ccggctacat cgagaaatac
     5041 gagaaatcga gcgatggccg cttttttatt cagctcaagg gtatcaaccg ctttagattc
     5101 caaaaggagc tcgaaatgcg gcgcggctat cgccgcgtta aagcgcttta tagcgaattt
     5161 cccgacgcga gcctgccgga ggattggcac tgcgagcgcg aagccatact gcaggcgctc
     5221 acgttattcg gccaagctca cggcatgcag gtcaaaccgg agcaggcgca gcgcttctcc
     5281 gatttggaac tggtgaatct tgtgtcggtg tcgttgccgt ttcaccccgc ggagaaacaa
     5341 gcattgttgg aagcgcaaac tctaaaagat cgggagaaca cgctcatcaa tttgcttcga
     5401 ctcggcgcgg gtcaagcgga tccggattcg gataccacac ctcgcacctt gaactgattt
     5461 caggctgctg aaaagactca tatgctgcgt tgcgctcagt cttctcgctt cgacgtactg
     5521 gaagtacgct tccccgcgtc gatctacgcg cgccttgcct atgagatgtt ttgagcagcc
     5581 tgcggcgctg actcgcagag catctcaatt ctccgagaga tcgatcagca caccatcggg
     5641 atccgatatg aagtgttggc ccaggcaaca tttgcggaaa tcgttttcga tcagtctgcc
     5701 atgcaggccg acggtgagtt ttcgcggcgc cgcctccggc gaagaaacgg ttaacgcatc
     5761 gagatcatcg gctgcggctt tgagactctc cactttgaag ccaacgtggt caagaccttc
     5821 tcgcaggccg cggtaaaggc tgttgtcgcg aggccgcagc aataatttga ctttgccgtc
     5881 ggtcatgcag attgatccgt cgtcgcgata agtctccgct tctttcagct cgaagatctt
     5941 ttcgtagaac tccgccacag ccatgggatt atcggagcga atcgatatgt gattgatcca
     6001 gcgcgattga tcccagccgc tttcgttgta gccttcgcgg acattgccga cgtgcgcttg
     6061 cgcgatgtcg aactgagtgc cggcgggatc atagctgcgc aatccggcaa acggcacata
     6121 ctccaatcct ttggcgatct ttacgtccgg atagtattgt tccatccggc gaacgaaatc
     6181 cttgatatcg gtgacttcga atccataatg gtccatgccc gactgtatcc ctgcgtatcg
     6241 cggcaggatg gccatgccga tgacgccgtc gctgatgtga ccgcggttgg cattttgttt
     6301 gccgctttca tcgacagtgc cgctggtcat ccgtttcatg ccgaagatcg tctggtaaaa
     6361 atgggtcacg gaagccagat catgagtata cattgctaga tggcgaattt ttgcgaacat
     6421 ggcggacctc ctcatgagta cacggtggag aaagcttctt ctaggttagt gttcctgaag
     6481 ttgtcaagaa ggcgtcgaaa agttgtgcgt ttccggtcca atgttttgcg tcagctcgtt
     6541 tctttttgct agcgatatag tttagcgtag aaaccgttat cctcaagttc cttgacgaaa
     6601 cgaacgtcga taaaatcgtc gggttttgca ttgctcgctt cgggcgccgt cttggcgaga
     6661 aaatctaaga gcgtctgaat gccgggtttg ctcggataag gaattttggg cgtcgctttc
     6721 aagaccactt cggtataagc ctccttgatg gccttttcgt cgccgtggag atacttccgc
     6781 atgattctct gcgcgccttc ggaattcgta tgaatgtaat ggttgccttc tgagtacgcc
     6841 atggcaaagc cgcgaagtac gtcgggccgc ttgttgatgt atgaacgcgt cgtgtaaacg
     6901 ccgttttgct gatagtggat gtcgatgtcg gcgaagttgc tgagaatccg aaagcctaac
     6961 tcttttgcct tgaccgtttc cggcggaatc aacagcgcgc cctggaccga accattcacc
     7021 agagcggcga agcgcgccgg taccgagcct acctgtacta tgttcatttc tttgtctggc
     7081 tgaacgccga gcttgggcag gacgaacagg gttgccgtgt
//
LOCUS       DASUVS010000224         6381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1183190, whole genome shotgun sequence.
ACCESSION   DASUVS010000224 DASUVS010000000
VERSION     DASUVS010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183190"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VF182_12550"
     CDS             <1..429
                     /locus_tag="VF182_12550"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6437958.1"
                     /translation="PEELKSLQDRSTDLIILDVRTPEEYARFCIPGGINVPGGDLILW
                     AEELKQRKATNVIVNCAGRTRSIIGAAALRRLGVGNVRALRNGTMGWVLAGFELEDKP
                     ERTPPPAPATDAEAATAVARRIAEEESIPWISALELSRRL"
     gene            448..1173
                     /locus_tag="VF182_12555"
     CDS             448..1173
                     /locus_tag="VF182_12555"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6437959.1"
                     /translation="MIDVRSQGEFAGGHVPESINVPGGQAVQRADDFIAVRNGQIVFI
                     SNESARAVMAAYWYRHMGFSHLAVLQGGLRGWVESGGILEKGSIRNEPLGFKAARRVA
                     RVDPRGVEVKTRDQSVLLLDVGTSLDFETSHIPGAKWISRGWLELKLPELYPDRNQPM
                     VIACRDGRQSLLAALTLGELAYSDVVVLDGGVSAWSAAGFHTQQGLDGCLVEPNDVVL
                     SPSIRGTKEDMQRYLDWELQLKK"
     gene            complement(1232..1798)
                     /locus_tag="VF182_12560"
     CDS             complement(1232..1798)
                     /locus_tag="VF182_12560"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX6437960.1"
                     /translation="MTTSYSLQDFIEDLDRITRAESAQENIVAKAKPLLAKLIQQRDC
                     IDEKYRRRGATAYGRYMLHRAPLFNVSSVVWGPGDNAKAHNHDTWGLVGVIENEIQET
                     RFRRKDDGTRNQYADLEVTAVVSNQAGMVSCLVAPSDDIHEMNNVTDRNTVEIHVYGK
                     DLTDMPRLRFDTAAKAVKTFASPKYDNC"
     gene            2125..2958
                     /locus_tag="VF182_12565"
     CDS             2125..2958
                     /locus_tag="VF182_12565"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6437961.1"
                     /translation="MPTANLNGVQIYYEDSGEGEAVLLSPPSWWPCDTWKVKVVPVLS
                     QRYRTIIFDCRGTGFSSKPDHGYTISQFAKDCAGLIEHLKISRVHAAGFALGGQIAQA
                     LAIERPDLVATLTIAAAGAGTKATSGAPREIRNTDEEEIREHGFERFIRSHVENTHMA
                     FNAIFYKEHPEVVRALSDALWQRQTSPEQHRYHYEARRTWDTLGNAPKIKVATLILCG
                     AEDNVNRGGSTPVATARRLAELVFGHELALVPDVKHMTFWDGEGALIALQNFLQRHPI
                     R"
     gene            3013..4071
                     /locus_tag="VF182_12570"
     CDS             3013..4071
                     /locus_tag="VF182_12570"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6437962.1"
                     /translation="MTFRAFVAAVTVGFLTLFVSAAGYAQGKGQSSDGIAELYNAARK
                     EGRVVIWGPTDAIVYQRMQEALDKQYPGIKIESFESIPEPLVQRIIAESQAGKPAAVD
                     VIQSGSLRALRPLIDRDMLAPYPGWEKLFGSDAVYAGQRFVGGYNLTLPIAYNTKTVS
                     AQDAPRSWEDLADPKWKGRKIIIEARLVPFAMLGTEWGKNKAIELTKKIISQQQPLIV
                     QGGTTVANALAGGQVSLAVGTYAFTIEGLKKQGAAVDWLPVAPLPVLTSAEGVLKTAP
                     HPNAARFFAGWMGTPEGQKIRYATRGQAMEVGRNAIGNVAERIRNEKPAIILETDKTF
                     ASILDIQRELGKLLGALR"
     gene            4241..6022
                     /locus_tag="VF182_12575"
     CDS             4241..6022
                     /locus_tag="VF182_12575"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6437963.1"
                     /translation="MSLSPTTLDKRFTTRSWSWTRNLSVRSFAFGSTVAVVSYLVLVP
                     LIMLLYASVKSTEDKLPFETTGMTLANYVTVLNSPSTLPIFLNTFLFSAGSLAVGLPL
                     AVVMAWLLERTAMPARRWIAALILVPMTIPSLLSAIAWIQLLDPRIGLVNIVLRNVLG
                     LSGDSGPFDIYTLSGMCFVQGLRLVPSAYLMIAASFRAMDPSLEEQSAMAGRGVGQTT
                     LRITLPVMRPALLAMLIYYIIVVIESFDIPGLLGFTARIRVLSTAIYWATHSEVGLPD
                     YGLASALGALVLALALLLMWVYQRLTKHQERFTTITGKGYRPRQIDLGAWRAPATALC
                     VFYVALAVVLPFLMLIWTSVQPFYAVPSAEALGRITLDGYVKIWHESTITRALWNTTL
                     LALATSAATILLAVLVSWFVVRRTRVADGLSYYLATVSFLPQCVPSIVIGLAFIFVYV
                     RFPIPIYGTLWIIVLAMTTRYLAYSSRTMTSALMQVHGELEEASQMSRAPWARTLRKI
                     TMPLLAPAMINVFFWVAVHAMQELSMALMLYNPDTVVISTMIWSMWQNGRTTDAAALG
                     VILIILSAVLLLGGLLFARFRRGPESV"
BASE COUNT         1324 a         1726 c         1858 g         1473 t
ORIGIN      
        1 ccggaagagc tcaagtcgct gcaggatcgt tcgacagatc tgattatcct cgatgttcgc
       61 actccggaag agtacgcccg cttctgcatt ccgggtggca tcaacgttcc tggcggtgat
      121 ctgattctct gggccgaaga attaaagcag cgtaaggcga caaatgtcat cgtcaactgc
      181 gccggccgca cacgcagcat cattggggcc gcggcgttgc gccggctcgg cgtgggcaat
      241 gtccgggcgc tgagaaatgg aaccatgggt tgggtgcttg ccggttttga gctggaagat
      301 aagcccgaac ggacgccgcc gcccgcgccg gccacagatg ctgaagcagc aacggcagtg
      361 gcgcggcgca tcgccgagga agaatccatc ccgtggatct ctgctctaga gttgtcgcgc
      421 aggctgtaaa ccaaaaccat cgattacatg atcgatgttc gctcccaagg cgagttcgca
      481 ggcggtcacg tgccggaatc gatcaacgtt cctggcgggc aggcggtgca gcgggccgat
      541 gatttcatcg ccgtgcgcaa cggccagatc gtcttcatca gcaatgaatc cgcgcgcgca
      601 gtgatggcgg cgtattggta ccggcacatg ggtttctccc atctagcggt gctgcaaggc
      661 ggactgcgcg gatgggtgga gagcggcgga attctcgaga aaggatcaat tcgaaatgag
      721 ccgttgggtt tcaaagctgc gcggcgggtt gcgcgcgtcg atcctcgcgg cgtggaagtt
      781 aaaacgcgtg atcaatcggt tttgcttctc gacgtcggca cgagtctcga tttcgaaacg
      841 tcacacatcc ctggtgctaa atggatttcg cgcggttggc tcgaactgaa attgccggaa
      901 ctttaccctg atcgaaatca gccgatggtc atagcttgcc gtgacggccg ccagtcgcta
      961 ctcgcggcgc tgacgctcgg cgagttagct tacagcgatg tcgtggttct agacggcggc
     1021 gtcagcgcat ggtcggcggc cggatttcac acacaacagg gactcgatgg ctgcttggtc
     1081 gaacccaacg atgtagtctt atcgccttcc attcgcggta ctaaggagga catgcagcgt
     1141 tatttggatt gggagttgca gctaaagaag taatcgcgtg agattggctt gattgtaagt
     1201 gaactctgcg ccgtgcaaat tgccgtaaca ctcagcagtt gtcgtacttc ggactggcga
     1261 acgttttgac cgctttggcg gctgtgtcga agcgcaatcg cggcatgtcc gtcaaatcct
     1321 tgccgtaaac gtggatctcc acggtattgc gatcggtaac gttgttcatt tcatggatgt
     1381 catcgctagg tgccaccaga caagaaacca ttccagcttg gttacttacc acggcggtta
     1441 cttcaaggtc ggcgtactga tttcttgtgc cgtcgtcctt gcggcgaaac cgagtttctt
     1501 gaatttcatt ttcgatgaca ccgaccaggc cccatgtgtc atggttgtga gccttagcgt
     1561 tatcgcccgg gccccaaacc accgaggaga cgttgaatag cggcgcacgg tgcagcatgt
     1621 agcggccgta ggctgtggcg ccgcggcgcc tgtatttctc atcgatgcag tcccgttgct
     1681 gaatgagttt ggcgagcaag ggctttgcct tggctacgat gttctcctgc gcgctttccg
     1741 cgcgtgtgat ccgatcgaga tcttcgataa aatcttgcaa tgaatagcta gttgtcatgg
     1801 tgattgtcta gcacgaatcg caaaaggtca aaagaaggcg catcttaggc gcccgttgga
     1861 tctttcttgc gctaacagag atgctaatgt gattggagtt aatccacgca ggaagtcatg
     1921 gcgcccaaaa tcgagagcgg agcatgcggc ggtgcttgac cctgtgctag gcgtgaaacc
     1981 tgaccacggc gaaagtacat cccgaatccg ctgaaccacc acaatagctg atgtaatctg
     2041 ttgacagcca aatccaattt ggattaggtc tcgaacgaag cgattacaga catgggtcca
     2101 aacctaatcg attaggaatg aaatatgccg acggccaatt tgaacggtgt gcagatttat
     2161 tacgaggatt caggagaagg cgaagccgtg ctgctatcgc cgccttcctg gtggccgtgc
     2221 gacacctgga aggtcaaggt cgtgcccgtt ctttcacagc gctaccgcac gatcattttc
     2281 gattgtcgcg gcactggttt tagcagcaag ccggatcatg gctacaccat cagccagttc
     2341 gctaaagact gcgccgggct tatagaacat ttgaaaattt cccgagttca tgccgcggga
     2401 ttcgcgctcg gcggacagat tgctcaggct ctcgccattg aacgacccga tctcgtcgcc
     2461 accttaacga tcgcggccgc aggggcggga acgaaggcca ccagcggcgc gccgcgcgag
     2521 atccgcaata ccgatgaaga agaaatccgt gagcatggct tcgagcgctt catccgaagt
     2581 cacgtcgaaa acacgcatat ggcgttcaat gcgatatttt acaaagagca tccggaagtg
     2641 gtccgcgcgc tctccgatgc gctgtggcaa agacagacga gcccggagca gcaccgctat
     2701 cactacgagg cacggcgcac ctgggacacg ctcggcaacg ccccgaaaat aaaagtcgcg
     2761 acgttgattc tttgtggcgc ggaggacaac gtcaatcgcg gcggcagcac tccggtggca
     2821 acagcgcgcc gacttgccga gcttgttttc gggcacgagc ttgcgctcgt gcccgacgtc
     2881 aagcatatga ctttttggga cggggagggc gcgctgatcg cattacaaaa cttcttgcag
     2941 cgtcatccga tcagataact tgaactttga accattgaac aggcgtacaa aagaattggg
     3001 agatatcggc ccatgacatt tcgagcattc gtcgccgcag tcactgtcgg cttcttgact
     3061 ctgttcgtca gcgccgcagg atatgctcaa ggcaaaggcc aaagcagcga tgggatcgca
     3121 gaactttaca acgccgcaag gaaagagggc agggtggtca tctggggacc gacggatgcg
     3181 attgtctatc aacggatgca agaggcgctg gataaacagt atccaggcat caagatagaa
     3241 tccttcgaga gcataccgga gccgctggtc cagcggatca tcgccgaaag ccaagccggg
     3301 aagccggctg ccgtcgatgt tatacagtcg ggttcgctgc gggcactgcg cccgctgatc
     3361 gatcgcgata tgcttgcgcc ctatccggga tgggagaaac tatttggctc tgacgccgtt
     3421 tatgccggcc aacgctttgt cggcggttat aatctcacgt tgccgattgc ctataacacc
     3481 aagacggtga gcgcccaaga tgcacccaga agctgggagg atcttgccga tccaaaatgg
     3541 aagggtagaa agatcattat cgaggcccgt ctggtgccct tcgcgatgct cggcacggag
     3601 tggggcaaaa acaaggcgat agagctgacc aaaaaaataa tttcacagca gcagcccctg
     3661 atcgttcaag gcggcaccac cgtagccaac gcgctggccg gcggtcaggt ttcgcttgcc
     3721 gtggggactt acgctttcac tatagaaggt ttgaaaaaac aaggagcggc ggttgattgg
     3781 ctcccagtcg cgcccttgcc ggtgctgacg tccgcagagg gagttctcaa aactgcgccg
     3841 catcccaatg cggcacggtt tttcgcagga tggatgggaa cccctgaagg gcagaagata
     3901 cgatacgcca ccaggggcca agccatggag gtggggagaa acgcaattgg caacgttgcc
     3961 gaaagaatca gaaacgaaaa accggcgatt attttggaaa cggataaaac ctttgcgtcg
     4021 attctcgaca tccaacgcga gctcggcaaa ctactcggtg cgttgcgcta gtgcgcagta
     4081 ttcggctaac cgctaaactg gcggggtgtc atccggagcg cgccgcgacg cgctacaggc
     4141 tagccgtcgc cagatctaat tcgctacaac tgacaaagct ctttacgtga tgttctcagc
     4201 atgactttgt tatactgagg attaaaaaca agttccttcc atgagcttat cgccaaccac
     4261 tctcgacaag cgcttcacca cgcgatcgtg gtcttggacg cggaatttgt ccgtccgttc
     4321 tttcgctttc gggtccaccg tcgccgtcgt ttcgtatctt gttcttgtgc cgctgatcat
     4381 gttgctttac gccagtgtca aaagcacgga agataaatta ccctttgaaa cgaccggtat
     4441 gacgctggcg aattacgtca cggttctgaa cagtccttcc acgttgccga tatttttgaa
     4501 tacgtttctc ttttccgccg gttccttggc ggtaggtctg ccgcttgcag ttgtcatggc
     4561 gtggctttta gagcgcacgg ccatgccggc gcgccgctgg atcgccgctc tcattctcgt
     4621 tcccatgacg attccgtcgc tgctgtcggc aatcgcatgg atccagctgc tggacccgcg
     4681 cattggcctc gtaaacatcg tcttgcgcaa cgttctcggt ttatcgggag actccggacc
     4741 gttcgatatc tacacgctct cgggcatgtg cttcgttcaa gggttgcgtc tggtgccatc
     4801 ggcgtatctc atgatcgctg cttcgtttcg cgccatggat ccatcactcg aagaacagag
     4861 cgccatggcc ggccgcggcg tcgggcagac gaccttgcgc atcaccctgc cggtcatgcg
     4921 gccagcgctg cttgccatgt tgatctacta tattatcgtg gtcatcgaat cgttcgacat
     4981 tcccgggttg ctcggtttca cggcccgtat tcgggtcctc agcactgcga tttattgggc
     5041 gacccactcg gaagtcggat tgccagacta tggtttggcc tcggcgttgg gagcgctggt
     5101 gttggcgctg gcgctgttgc tcatgtgggt gtaccagcgc ttaacgaagc accaggaaag
     5161 attcaccacg atcaccggca aagggtatcg tccgcggcaa atcgatctcg gcgcgtggag
     5221 ggcgccggcg accgcgcttt gtgttttcta tgtggcgctg gccgtggtgc tgccgtttct
     5281 catgctgatc tggaccagcg tccagccgtt ctatgcggtg ccttcggcag aggcgctggg
     5341 gcggattact ctcgacggat acgtaaagat ctggcacgag agtacgataa cccgcgctct
     5401 ctggaatacg accttgctgg cgctggcgac ctccgcggcg acgatcctgc tcgccgtcct
     5461 ggtaagctgg ttcgttgtcc ggcgcacgcg agtcgcggat gggctctctt attatctcgc
     5521 aaccgtgtca tttctgccgc aatgcgttcc gagcatcgtc atcggtctgg cgtttatctt
     5581 cgtttacgtc cgctttccca ttccgatcta cggcacgctc tggattatcg ttttggcaat
     5641 gacgacccgg tatctcgcgt atagttcgcg cacgatgacg tccgcgctca tgcaggtgca
     5701 cggcgagtta gaggaagcat cccagatgtc gcgggccccc tgggcgcgca ctctgcgcaa
     5761 aattaccatg ccgctgttag cgcctgccat gatcaacgtt tttttttggg tcgcggtgca
     5821 cgcgatgcag gagctgtcga tggcactgat gctttacaat ccggataccg tggtgatttc
     5881 gacgatgatc tggagcatgt ggcaaaacgg ccggactacc gatgccgcgg ccttaggcgt
     5941 gatcttgatc atcctctccg ccgttttgct gcttggcggg ttgctgtttg cgcgcttccg
     6001 gcgcggtcct gaatccgtat aggcgggctt gaaacttgtg tgcttcgttg caatcaagcg
     6061 acttcaatca acccacgaat gaatacatct acgcttagcg ataatggccc actcgcatct
     6121 ggatctgtgt gcgcagcctg cttgggcttt tatgggaagc ggttttttcg gtatcggatc
     6181 tcttggcgag cttggcacct ggattgattg ttgtcggatg actgctcccg tcaagttgca
     6241 aacgctgcaa agattcggaa caagatgatc ggtgttagag atctggaaaa gtcgtttgcc
     6301 ggcgccagag gtgaagtgcg cgcgctgcgg ggtgtttctt tcgaagtaag caaaggcgaa
     6361 ttgtttacct tgctcggtcc a
//
LOCUS       DASUVS010000225         6857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_309564, whole genome shotgun sequence.
ACCESSION   DASUVS010000225 DASUVS010000000
VERSION     DASUVS010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309564"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VF182_12580"
     CDS             <1..732
                     /locus_tag="VF182_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437964.1"
                     /translation="YYYSTQERILFEQLLASGHVPKMAIFLDGINDFCFDKDDSTFSE
                     RFSNAFEGHDRGHARSWLVPWLSGLPVGRAVESAKRRLSMAKDSGPQGPSIDSRADEA
                     MYNDAAVIRHVIDRYLQNKKIIESTSRIYDVTPVFVWQPAPAYKYDLRYHSFAANGLG
                     VHTRHRYGYAALADMIRDRPLEGNFLWLADIQEQSQESLYIDLIHYSPAFLKELATQI
                     ESRLTELGVYDQALQPRYDSAGPPR"
     gene            753..941
                     /locus_tag="VF182_12585"
     CDS             753..941
                     /locus_tag="VF182_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437965.1"
                     /translation="MSFPLGSIYRGEACWRIIELLEVGFRHQRQLSKDERQMQKSETL
                     LQPAVIRLMVRRVARSIV"
     gene            1561..2658
                     /locus_tag="VF182_12590"
     CDS             1561..2658
                     /locus_tag="VF182_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437966.1"
                     /translation="MQPAHDDQSYDILFLGGSVLDSDWGQVEQSLREQLAYAGQRNVR
                     IFNLASAAHTSRDSWLKYAALSEARFDLVVFYHGVNDSRANNSPPEEFRENYGHFSWY
                     EAVNTLAQYHGQAMFALPYTLRWLVIRTRQVVMKDRYVPTREPRADWVRYGQESRSAV
                     SFKHNLEAILDLASQRGDQVLLMTFASYVPADYSLERFKEKRLNYGLHLSALEIWGLP
                     QHVLATVAVHNEIVRWLATQHKQILFVDQASLMTGSALYFNDPYHFTVLGSIKFVENI
                     VRALLPRPDSNRTRKEKLSLFAATNQFLGKQRSPRGRGHWMPMPEVTEKSVVRYAGGS
                     DSYRGGIPERLDDGRTGFLVPVGTLEHSLSD"
     gene            3123..5546
                     /locus_tag="VF182_12595"
     CDS             3123..5546
                     /locus_tag="VF182_12595"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEX6437967.1"
                     /translation="MSDNRSVPSLITLLNQAIARVARLAAPVLLDVVCLMITPLIVRL
                     SLLHSEGIRLQRHDVKWLISGLAVVFVAVGLLSLIPKRRRFPFFATISVLWAIAHYVN
                     YETYLALGGVLELAFLPLLFDPTFFVGSATHVSRPVLLIASLVLPIVVAWGLEKKTPS
                     CGYPKVSCVTAIVIAILISMSHKTNILVATWRQTDPVYYNIRELTSHLQIKLFGGAGV
                     SAAADSGVAVVERDPLNPLAPSAESWSRQLKLLRLSAFTNLAPDFKEEVAKTFELDLN
                     GKRWLETAHKKTPNVLFIVIEGLSAAYFREAATSNSFDSSRHFRETEAVLAAQPHVFY
                     QNFMAHNRQTSRGQYAIICGRFPNVLVRDLEFNNIIKDASENKLCLPEILRRKGYSTA
                     FVQASTISFLNTDRFLEKAGFEVIKGNESFSNRRAFGFWGVDDATLYSKALDTIAAMD
                     GARKPWFVVAETIGTHHPYPIPDEFKKKTKLTDAAAAFAFADQELADFLRELERRKIF
                     DSTIVFIMSDESQGNTSLKDKDIMSTASQSWIPMIVIAPEKISHKVQDIYTHRDLAVS
                     VLDYMAEDLRKYNLQGRSMFRTYGHERVIPFGNIYHQRVGMIDPRGFYLICDYEFSAC
                     SQFKRARDSVFPEEGQRLPFDESQVKELQYLLFLNEYRDDAPVLLENQNVALVRLGPR
                     DVESKEKFDFIGRRYFNVPPFQAACLVLDFALQKGELALSVDFTVGHGKSFPFSTSWV
                     VLEEGEKFKGQACYAGGPSGIVDLEPRVIVYNKAADKSVLDVRNAAFSLQKSSKASHH
                     VINDFRKID"
     gene            6386..>6857
                     /locus_tag="VF182_12600"
     CDS             6386..>6857
                     /locus_tag="VF182_12600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6437968.1"
                     /translation="MLTSAVMETRVIPNGVDLSIFHPADKKDARSVLGIPQNALVILF
                     TANAVRRNIWKDYRTVEDAVKLAATRTKEQNVLFLALGQEGKGERIGPAEVRFVRYQQ
                     DPEVVARYYQAADVYVHAARADTFPNTILEALACGTPVVATAVGGIPEQIEDGRT"
BASE COUNT         1584 a         1775 c         1840 g         1658 t
ORIGIN      
        1 tactactatt cgacccagga gaggatccta ttcgagcagc tgctggcgtc cggtcatgta
       61 cccaagatgg caatattcct cgacggtatt aatgacttct gttttgacaa agacgattcg
      121 acgttctccg agcggttttc caacgcgttt gaggggcacg accgtggaca cgcgcgctcc
      181 tggctggttc cttggctttc tggcttgcct gtgggccgag cagtggaatc tgctaagagg
      241 cgcctaagta tggctaagga ttccggccca cagggcccga gcatcgattc ccgtgcggac
      301 gaggccatgt acaatgacgc agcggtcatc cggcacgtca tcgatagata cctgcagaat
      361 aagaaaatca tcgagagcac cagtcgcatc tacgacgtga ctcccgtgtt tgtgtggcag
      421 ccagcgcctg cctacaagta tgacctgcgg taccattctt tcgccgctaa cggattaggt
      481 gtccacactc gccaccgtta cggctacgcg gctctagcgg acatgatccg cgaccgtcct
      541 ctagaaggca attttctgtg gctggccgac attcaggagc agtcccagga gtcgctttat
      601 atcgatctga tccattactc gcccgcgttt ctcaaagagc tggcaactca gattgagagc
      661 cggctaacgg aactgggcgt gtacgatcaa gcactgcaac cacgctatga tagcgccggt
      721 cccccaaggt gaaattgatt ggcgctatct ttatgagttt cccccttggg agtatctatc
      781 gaggagaggc ctgctggcgg atcattgaac tgctcgaagt cggcttccgc caccagcgcc
      841 agctgagtaa ggacgagcgc caaatgcaga agagtgaaac gctgctgcag cccgccgtga
      901 ttcgactgat ggtgaggcgc gtggcgagaa gcatcgtatg attttagaac agagcacgct
      961 ctcaccggag taggtgctcg accagggtgc gctgctcgtc agcaatgatg ccctcttcgg
     1021 cgctagctac aaactggcgt ccacagcttt gcaccggtga ttctgcttgc ccataggaat
     1081 atggccgtct tctgaactgt atcaatccac accctggaca cgcgtccctg acactcatga
     1141 ctggcgtggg cctgcttcgg gggtaatgtt ttgaccacgg tagcacggat ttggatcgtg
     1201 gggaattacc tgtgtagcgc taccatctac cgtgtacaac ttggtgcgcg tgcccttgga
     1261 tagtggcgag gattccatat gcggtttgtt acaaataaaa tcaaggctat ggccctgttc
     1321 ttcaggggtg atgatgtgtc aggacgccgt accgggagag ccgattcaat tcaaggttat
     1381 acgatgcgcc acaacggatg gcgcactacg ctcccctcgc tcttaatctc agcggtccta
     1441 cttgttgtgg gtgcggaagc cgcagcgcgg attttctggc gcctctgtta cggcattcca
     1501 attggtcacc cggatcgcat cctgtatgcg tactacccag ctctacgaga ggtggacaag
     1561 atgcaaccgg cccatgacga tcaatcctat gacatcctgt ttctcggggg ttcggtgctt
     1621 gatagcgact ggggccaagt ggagcagagc ctccgtgaac aactggccta cgccggccag
     1681 cggaacgttc gtatcttcaa cctggccagc gccgcgcata cctcgcggga tagctggctt
     1741 aaatatgccg cgttgagcga ggcccgcttt gatctcgtgg tcttctatca tggagtcaat
     1801 gacagccgtg cgaacaattc tccgccggaa gaattccgag aaaactatgg gcatttttcc
     1861 tggtacgagg cggttaacac tcttgcccaa taccacgggc aggctatgtt tgctttgccc
     1921 tacactctgc gttggcttgt catccgaacg cgacaagtcg tgatgaagga ccgttacgtc
     1981 ccaaccaggg agccacgcgc agattgggtt cgctatggac aggaatcacg cagcgcagtg
     2041 tctttcaaac acaatctgga agctattctc gatctcgcct cgcagcgtgg cgatcaggtg
     2101 ctgctcatga cgttcgcgag ctacgtcccg gctgattact ccctcgaacg gttcaaagaa
     2161 aagcggctca actatgggct gcatctttcc gcgctcgaaa tctggggact acctcagcac
     2221 gtactggcaa cggtggctgt tcacaacgag atagtgcgct ggctggccac ccagcataag
     2281 cagattcttt ttgtagacca ggcgagcttg atgactggct ccgccctcta cttcaacgat
     2341 ccttaccact tcactgtcct cggatccatc aagtttgtcg agaatattgt cagagccttg
     2401 cttccaagac ccgacagcaa tcggacgaga aaagagaagt tgtcattgtt tgctgcgacc
     2461 aatcaattcc tgggtaagca acgcagtccg agaggtagag gacactggat gccgatgcca
     2521 gaagtaaccg agaaatccgt tgttcggtac gccggtggga gcgacagcta taggggtgga
     2581 attcctgaac ggcttgatga cgggcgaacc gggtttttgg tgcccgtcgg cacgctcgag
     2641 cattcgctga gcgactgaca caattgttat cagataatgg cctcaccaac gcatgaggat
     2701 attggccgct aaagctgcgc gccggcaatt cgatctgcag cactgggcag aggcatatct
     2761 tgattggtac aaagagcttg tggaacagca aacagctgag cgccgggcaa ggagaaacta
     2821 acgtgcgctc cccaatgttt aaccaactta ccccgcctcc gccgagcaaa gccagccacc
     2881 ataaaccgga gaatctcccc ggttgttaga aatagggctt ccgggggcaa caccggtcgc
     2941 acgttggcgt tgttacaccg tcatacgatc aaggagaatt gatcgaagag actattcggt
     3001 gctgcgataa tatcggagcg gttacgatga agatgaatcg aagcaggatg acccagaaca
     3061 ggcgctggac ttgcccgcta tatatccggc aaatttagcc aacacactgg aagcacacaa
     3121 ctatgtccga caacagatct gtgccttctc ttattacact actcaaccaa gccatcgcac
     3181 gtgtggcgag attggctgct cccgttctcc tagacgttgt gtgcctgatg atcacgcctt
     3241 tgatcgttcg gctaagtctt ctgcattcgg aagggattcg attgcagcgg cacgacgtga
     3301 agtggttgat ctcgggctta gccgtcgttt ttgtggcggt gggacttttg tctctaatac
     3361 caaaaaggag acgattcccc tttttcgcga cgatctcagt tctgtgggcg atcgctcatt
     3421 atgtcaatta cgaaacctat ctggccctgg gtggagtttt ggaactcgcg tttttgccgc
     3481 tgctctttga tccgactttt ttcgtcggtt cggcgactca cgtgagcaga cccgtgttgc
     3541 tcatcgcatc gctggttttg cccatcgttg tggcttgggg cttagagaaa aagacgccat
     3601 cctgtggtta cccgaaagtt tcgtgtgtga cagcgatcgt catcgctatc ttgattagca
     3661 tgagccacaa aacaaatatc ttggtggcga cctggaggca gacagatccc gtctattata
     3721 acatacgcga acttacgagc catttgcaga tcaaactttt cgggggagct ggagtaagcg
     3781 cggctgcgga ttccggggtg gcggtggttg aacgagatcc attgaaccct cttgctccat
     3841 ctgcggagag ctggagtcgg cagctaaagc tgcttagatt gtcggcgttc acgaatcttg
     3901 cgcctgattt caaagaagag gttgcgaaaa cgttcgagct cgatcttaac ggcaaaaggt
     3961 ggcttgaaac agcccataaa aaaaccccaa acgtcttatt catcgtcatc gaggggttat
     4021 ccgctgcata ttttagagaa gccgccacaa gcaattcttt tgacagcagt cgacattttc
     4081 gggaaaccga agcagtcttg gcggcgcagc cccacgtctt ttatcagaat ttcatggcgc
     4141 acaatcggca aacaagtcgt ggtcaatatg cgattatttg cggacgtttt cccaacgtcc
     4201 tcgttaggga tctggagttt aataacatca ttaaagatgc cagcgaaaac aaactttgcc
     4261 tcccggaaat tcttcgacga aagggttaca gcacagcttt tgtccaggcc tcgacaattt
     4321 cgtttctcaa tacagatcga tttctggaaa aggccggttt cgaagtgatc aagggcaacg
     4381 aaagtttttc gaatcgtcga gcttttggat tttggggtgt tgacgatgca accctatatt
     4441 ccaaggccct tgatactatc gctgccatgg atggagcacg aaagccgtgg tttgtcgtcg
     4501 ccgagacgat cggcacacac catccttatc caatccccga cgagttcaaa aagaaaacaa
     4561 aattgaccga tgcggccgcg gcatttgctt ttgccgacca ggaattggcg gattttttgc
     4621 gcgagctcga gcggcgcaag attttcgact cgacgatcgt atttatcatg tcggatgaat
     4681 cccagggcaa cacgagcctg aaagataaag acatcatgag tacagcctcg cagagttgga
     4741 ttccgatgat tgtcatcgct ccggaaaaaa tttcccacaa ggttcaagac atttacactc
     4801 atcgtgacct cgctgtctcg gttctcgatt atatggccga ggaccttcgc aagtacaatc
     4861 tgcaaggtcg aagcatgttt cgcacctacg ggcatgaacg cgtcattcca ttcggcaaca
     4921 tttatcatca gcgtgttgga atgatcgacc cgcgggggtt ttatctcatc tgtgattatg
     4981 aattttcggc ctgtagccag ttcaagcggg cgagggactc agtgtttccg gaggaaggtc
     5041 aaagacttcc tttcgacgaa agccaagtta aagagcttca atatctgctg ttcttgaacg
     5101 aatatcggga tgacgctcca gtactgctcg aaaaccaaaa cgtcgcgctg gttcgcctcg
     5161 ggccacgcga cgtcgaatcg aaggaaaaat tcgatttcat cggacgacgt tacttcaacg
     5221 tcccgccgtt tcaggcagcc tgcctcgttc tggactttgc attgcagaaa ggcgaactag
     5281 cgctatctgt tgacttcacg gttggccacg gcaaatcctt tcccttttcc acctcttggg
     5341 tcgttctgga agagggcgag aagtttaaag ggcaagcttg ttacgccgga ggcccgtcgg
     5401 gcatcgtgga tctcgaaccg cgcgttatcg tttataataa ggcagcagac aaatctgtcc
     5461 tggacgtccg gaacgcggca ttctcgttgc agaaatcatc caaagccagc caccacgtca
     5521 ttaatgattt tcgcaagatc gactgagtcg actgggtttg cttcccctcg gtcaccgatt
     5581 tgccgaattt ttattccaaa ggcattcagt gaactgagtt atcatgcagg aaagttgtgc
     5641 tgacctgatg ggactacctt agttactaag tcattataag cgttgcaagt ggtttatgat
     5701 taaccgaggg ttatgtatct tgcaggtcag cacgtacgat atcagtggtg gggcggagaa
     5761 ggtggccagc aacctgtttc agagctaccg atcaagcggc tatgattcat ggctggccgt
     5821 cggacataaa ctcggtatcg atccggacgt gttgcttgtt ccgaatcata aaagacgcgt
     5881 caggcaacgt cgtttacttt ctgataacca ttcgggctca cagtcaccca cactgcaggg
     5941 aggcacggtc tcatggctga gcgcatttgt tgatgtgata gctgagccgt gcagagggct
     6001 cgctcgttat tgtggaattg aagactttag tttccccgga acctggaagc ttctgaacct
     6061 gtccccaaaa ccaccagaca ttgttcactg tcacaatctg cacgggagat attttgatct
     6121 gcgagctctc ccttggttga gccagcaggt accggtaatt ttgactctcc atgatgcttg
     6181 gctgctcagc ggtcactgcg cccattcgtt tgattgcgag cgctggaaaa taggttgcgg
     6241 ccattgcccg gacctgacca ttgatccggg tatccgacgg gatgcgacgg cctacaattg
     6301 gcggcgaaag cgcgagatat tcaagaacag ccgactctac atcgctactc catgccggtg
     6361 gctgatgcag aaggtggaac aatcaatgct gacatcggcc gtcatggaaa ctcgagtcat
     6421 tcccaatggt gtcgatctgt cgatatttca tcccgccgat aaaaaggatg ctcgctcggt
     6481 tctgggcatt ccgcagaatg ctctggtgat cctctttaca gcaaacgcgg tgcgacgaaa
     6541 catctggaaa gattatcgga ccgtggagga tgccgtcaag ctagctgcta cccggacgaa
     6601 agaacagaac gtgctgtttc tcgctttggg tcaagaggga aagggagagc gaatcggacc
     6661 cgccgaagta cgctttgtcc gttaccaaca ggatccggag gttgttgcgc gttattacca
     6721 agctgccgac gtttacgtgc atgccgctcg tgctgacacc tttcccaaca cgattttgga
     6781 agctctggcc tgtggtacgc cggtggtagc aacagcagtg ggcggaattc cggaacaaat
     6841 tgaggacggg cggacgg
//
LOCUS       DASUVS010000226         3254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1621156, whole genome shotgun sequence.
ACCESSION   DASUVS010000226 DASUVS010000000
VERSION     DASUVS010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621156"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(619..1920)
                     /locus_tag="VF182_12605"
     CDS             complement(619..1920)
                     /locus_tag="VF182_12605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6437969.1"
                     /translation="MADTTPIDSLAGLTTQITGLFRNDLIYNDSETSAAENRKALIAL
                     QWFVAIGTSYLILSSQAWSLGAPLPLLLISVCILSAPALQRLPLKFFSGKNIESKLLV
                     FDSILILAAIGSTQSAPWDLLALFFFCVFIAATGENLLHIVVGCTLLSLMFLIFISSQ
                     QIDLWTINSDLLLRVPFMLGISVLYGHLTNEVKRDKRRIQQLKQTEEFKRQVVCALAH
                     DVKAPLSVILGHAELLAGSFGGQPNPAEQTLSLKCIRENIDRIVNLVIGFLNVSELVT
                     PKAESSKTAVEINPILRDVIQQQTIALRKKKLDLRLELAEDLKPCHGDHNQLERVLWN
                     LIDNAIKYTPPGGAIALTSRMVAGKISLSISDSGVGIPKAELPNLFREFKRFEGSANT
                     EGTGLGLFIVKTILDDHNGTITVESDRGAGTTFKVELPVRS"
     gene            2488..2871
                     /gene="crcB"
                     /locus_tag="VF182_12610"
     CDS             2488..2871
                     /gene="crcB"
                     /locus_tag="VF182_12610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX6437970.1"
                     /translation="MGPILYVGAGSFVGGILRYLLSTWIYKILGNPLFPYGTLAVNSL
                     GCLVIGFLAGLAETRSIFTANDRLFIFVGVLGGFTTFSSVALETLWLARGSQGLSAMT
                     NIALQLFLGMFAVWIGNLLAHQIAN"
     gene            2983..>3254
                     /locus_tag="VF182_12615"
     CDS             2983..>3254
                     /locus_tag="VF182_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437971.1"
                     /translation="MIAINKRMTNTLTLAAALLIVLPGCASQRPSLYSNEHLMRVGSS
                     QAERDIDQCVQQAEGATEGRESAGENAAVTTAGSAAIGAAAGGAGGA"
BASE COUNT          786 a          792 c          878 g          798 t
ORIGIN      
        1 gaaaatgtcg ccactgaaac gatcccagcc aattgttgtt gcgctcgaat attgacaatc
       61 gccggatctg tcttgatccc atgagtttgc ggctgtgttc aacggagtcg aattcgaagc
      121 agtaagcgat ctcactcgac atcacatccg aaacgcgcat ctttgacgga tcgcggcctt
      181 ccgccatcgc acgaagggtg atatctcaat cgctcaccaa accggacaaa ttctgcccat
      241 cacacacggg aattgctccg acatccagtt ccttcacctt tgccgcggcc ggcacgaatg
      301 gaagcatttg gcgggatgac gtctacatcg cccgtcacga tctctgtaaa tctgtataaa
      361 ccaccacctt gattttacgc acagaaaaca cagccatatt gcaaacatta cgcgtatagc
      421 tcacgtctcg gagctaagct gaaggctaaa cttcgatatt gggggaatcc gaaacggatc
      481 aggactacac aggttaaggt ccgcaggagc cgttcacgtg cgcagagtga taacagattg
      541 gaaaagcaga tcgcgtcgat caatcgtgct gacttcaggc acaagcagtc gagcgagagt
      601 tgcgttggct tggatggctc aactacggac gggcaactcc actttgaaag tcgttccggc
      661 acctcggtcg ctttcgacag tgatggtgcc gttatggtcg tctagaatgg tttttacgat
      721 aaacaatccg agtccggtgc cttccgtatt ggcggagcct tcgaaacgct tgaactctcg
      781 aaagagatta ggcaattctg ctttcggaat gccgacaccg ctatcgctga tgctcagcga
      841 gattttccct gcgaccatcc gtgaggtgag agcgatggcg ccgcccggtg gggtgtattt
      901 gatggcgtta tcgatcaggt tccaaagcac ccgctctaat tgattgtgat cgccatgaca
      961 gggctttaga tcctccgcca gttccaatcg gagatccagc tttttcttgc gtagtgcaat
     1021 tgtttgctgc tggatgacgt ccctgagtat aggattgatc tccaccgcgg tcttactgga
     1081 ttccgccttc ggtgtaacaa gctcagagac attcagaaat ccgatcacca gattcactat
     1141 ccggtcgatg ttttcccgaa tgcatttcag cgatagagtt tgttcagcgg ggttgggttg
     1201 gccgccgaag gagccggcaa gcaattcggc atggccgaga atgacagaaa gcggtgcttt
     1261 aacatcatgc gcaagggcgc acacgacttg acgtttgaat tcttcagttt gcttcagttg
     1321 ttggattcgt ctcttatctc gtttcacctc attggtcaga tggccgtata gaaccgatat
     1381 gccgagcata aacgggactc gcagcagcag gtccgagttg atagtccaca agtcgatttg
     1441 ttgtgagctt ataaaaatca gaaacatcag acttaacaat gtgcatccaa ccacaatatg
     1501 taacaggttt tcgccggttg ccgcgatgaa cacgcagaag aaaaaaagcg ccagcaagtc
     1561 ccacggcgca ctctgagttg atccaatggc ggcaagaatg aggatcgagt caaacactag
     1621 aagttttgac tcgatgtttt tgcctgagaa aaacttcagc ggtagccgct gcagcgccgg
     1681 agccgatagg atgcaaacag atatcaatag taagggtaga ggtgccccga ggctccatgc
     1741 ctggctggat aagattagat aagaggtgcc gatcgcaacg aaccactgga gtgcaataag
     1801 ggccttgcga ttttctgctg ccgaagtctc tgagtcgtta taaatcaagt cattcctgaa
     1861 caagcctgtg atttgagtgg taaggccggc tagtgaatcg attggcgttg tgtcagccat
     1921 tttgccgccg tgcgagcgca atcctagtct cagccaagtc attttgagca tttcaaccat
     1981 tgagtcctag aatactgagg ctagagttgt tcgaagcggt tcacagacat gatgcgatag
     2041 ttgatacccc gtgtcgagca acccatgtgc cagagtgcaa gatacagtag gttcaatgac
     2101 ttacgtttta gtaaagcggc tgatttatga caaaatggtt aatcgtcgga aaaggcggcc
     2161 aaaactgagc ctcgcagctt ggaatcgggg aagttaaagg ttgaggagcg catcaaagac
     2221 tctgatcgta tttcgctgaa tcggtgagtc tggtggaatc ggttctatcg ccgtgctctc
     2281 tcgggagcgc agatatggtt cccgctaccc ccagacaatg ccgaaggatt ttatggattt
     2341 gatcgtaacg ggatcgaggc tcagtgtaat cggctccaaa tttccgtatg aattcgaaat
     2401 ggccggcgtc cggcagctaa accgtttgcg cacgaagtta ccggcagcga aaaaatttcg
     2461 cgcacatcct caaggaggtt gtattagatg gggcctatct tgtatgtcgg cgccggcagt
     2521 tttgttggtg gcattttgcg atacctgctg agcacgtgga tctacaagat cctgggcaat
     2581 cccttgtttc catacggcac actcgcggtc aactctttag gatgtcttgt tattgggttt
     2641 cttgccggct tggcagaaac gcggtcgatt ttcactgcaa atgatcgtct ttttattttt
     2701 gtcggcgtgc tgggaggatt cacaaccttc tcgtccgtag cattggaaac gttgtggctg
     2761 gcgcgaggca gccaaggttt gtccgctatg accaacatcg cgctgcaatt gtttcttgga
     2821 atgttcgcag tttggatcgg caatctactc gcgcatcaga tagcaaacta atttcctaat
     2881 gctcggcggc acgaagcgta ggagagatca aagaccagtc gtgaatcgta tatatccgga
     2941 tgggcgggtt gacccgtgta ttatggcccc tagaatcaat gcatgattgc aatcaacaag
     3001 cggatgacca acactctgac gctcgctgcg gcgctcctga ttgtgcttcc gggatgtgcc
     3061 agccaaaggc cttcacttta ttcgaacgag cacttgatgc gggttggttc tagccaggcc
     3121 gagcgggaca tcgatcaatg cgtgcaacaa gcggaaggcg ctaccgaagg gcgagagagc
     3181 gctggggaaa atgccgccgt taccacggcg ggtagcgctg ccatcggagc agccgctggg
     3241 ggtgcaggcg gcgc
//
LOCUS       DASUVS010000227         4454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_309657, whole genome shotgun sequence.
ACCESSION   DASUVS010000227 DASUVS010000000
VERSION     DASUVS010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309657"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /gene="tssG"
                     /locus_tag="VF182_12620"
     CDS             complement(<1..797)
                     /gene="tssG"
                     /locus_tag="VF182_12620"
                     /inference="COORDINATES: protein motif:HMM:NF018675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEX6437972.1"
                     /translation="MATESRRADPSLEHVLFEEGYRFDFFQAVRVLERLYSARLPVGR
                     SSIPSKEAVRFRSLLSLNFPASSIYDVAQSDNGDSPPEMVVTFMGLFGLAGVLPRHYT
                     ELLLERVRQKDFALRDFLDLFNHRMISLFYRAWEKYRFPVAYERSRSSTGAGYDPFSF
                     SLFNLIGMGTDGLRGKLKSGDEPLLYYSGLIAQQPRSASALAAMLGDYFNVPVIIKQF
                     VGAWLRVDDQNRTRLGRGDFNNQLGTTTMLGSKCWDQQAGFTVALGPL"
     gene            complement(761..2581)
                     /gene="tssF"
                     /locus_tag="VF182_12625"
     CDS             complement(761..2581)
                     /gene="tssF"
                     /locus_tag="VF182_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEX6437973.1"
                     /translation="MPDDLITYYERELSFLRQMGAEFAAKYPKIAARLLLEGDASEDP
                     HVERLIQAFAFLAARVHHKIDDDFPEVTESLLNVLYPHYLAPIPSMSVAQFVLDPDQG
                     KLTSGYAIPKQSTLYSQPVDGAPCRFRTCYPVTLWPVQIEAASLEPVDSRLGVFPKAA
                     LGWIRLEIRCLSGIKFVQLKLDRFRFFLHGEGALTYALYELLFNNVMEVRIPSNKPNE
                     SARDISLPVSSVRPVGFAADEGILPYGARSFLGYRLLQEYFSFPEKFLFFELTGLDRA
                     VHDRSDDRMEIRLLLNRMPRLEQPVTRDTFRLGCTPIVNLFSQTAEPVRLSHTSTEYQ
                     VFPDIRRQNATEVYSIDSVTSVSPGSDEVVEFEPFYSFKHGLEREQQQAFWYAARKPS
                     PKKGDNGSDVHLSFVDLNFRPTLPAVDTLIIHTTCSNRDLPGKLPFGGDRGEFELEGA
                     APLSRIRCLRKPTEALRAPLRRGTQWRIISHLGLNYLSLCEAGLEALREILKIYDFAD
                     SSSVRQQINGITRLTSKQVVGRPSSMPWNGFCRGLEVTIEFDEEKFVGGGVFLFAAVL
                     ENFIGLYTSINSFTQLVAKSRQREEPIKRWPPRAGEQILL"
     gene            complement(2581..3072)
                     /gene="tssE"
                     /locus_tag="VF182_12630"
     CDS             complement(2581..3072)
                     /gene="tssE"
                     /locus_tag="VF182_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HEX6437974.1"
                     /translation="MARIDNDVNIVPSILDRLLDDEPEVSTEPATSRIKGFRSLERSV
                     ARDLEALLNSRQETLKEVPSEFKEVARSLLTYGLPDFTSFSLLTQDDRLRIRRSVEDA
                     INTFEPRLLRVRVTLEAPREHDRGLRFRIDAFLRVDPSPEPVTFDAVLQMNTQQYVIQ
                     REQ"
     gene            complement(3279..>4454)
                     /locus_tag="VF182_12635"
     CDS             complement(3279..>4454)
                     /locus_tag="VF182_12635"
                     /inference="COORDINATES: protein motif:HMM:NF045154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEX6437975.1"
                     /translation="KQNLADNGVAYVSYNTYPGGHMRDMAREMMRFHVSEFNDPQQRV
                     TQARSLIKFIIDAGSEPDLYRMVLQKELDRITEFADSSLFHDDLSEYNAPVYFYQFVE
                     HAGRHGLKYLSEAHFFEIQTGIFPARVVEVLNRISDSAIAKEQYLDFLKCRRFRQTLL
                     CHDDQELDDTPRPEKVTTFYIASPIRPADANACLDSRDQLTFSGPKNSSLQTDNPLIK
                     AAMSALGKAWPKAIHFQELLAQARGSCESASSCATAGANDDARTLGEVLLRAYAGGIV
                     EFSLAAPNVVTSAGLKPVASALARLQSLEGVEVVNLRHFNLRVEDPLSRRLLQLLDGS
                     RDRTALLDGLEVSTKSGDAAVEIKNGASTDNAETRCFLAIELERKLAELAEMGLLVA"
BASE COUNT         1075 a         1220 c         1211 g          948 t
ORIGIN      
        1 agcggcccca gggccacagt aaaacccgct tgctgatccc aacactttga tcctagcatg
       61 gttgtggttc caagctgatt attgaaatca ccgcgaccaa gcctagtacg attttgatca
      121 tcaacacgga gccaagcgcc gacaaattgc ttgatgatca ccggcacgtt gaaatagtca
      181 cccagcatag cggcgagcgc gctcgctgag cggggctgtt gggctatcag gccgctgtaa
      241 tacagcagag gttcatcgcc cgatttgagc ttgcctctga gaccatccgt tcccattccg
      301 atcagattga atagagaaaa cgaaaatgga tcataaccag cgcccgtgga cgagcgggaa
      361 cgctcgtacg caaccggaaa acgatatttc tcccaagcgc gatagaagag ggaaatcatc
      421 cggtgattga aaagatcgag gaaatcgcgc agagcgaaat ctttctggcg cacccgttcg
      481 agtaaaagct ctgtgtaatg tcgcggcagt acccctgcga ggccgaacaa gcccataaac
      541 gtgactacca tctccggtgg actgtcaccg ttgtcggact gagcgacatc ataaatcgag
      601 ctggccggaa aattgagaga aagaagcgac cggaaacgca ccgcctcctt ggaaggaatg
      661 gaactgcgtc cgacaggcag gcgcgccgaa tagaggcgct ccaggacacg caccgcctgg
      721 aagaaatcga accgataacc ctcttcgaag agcacgtgct ctaaagaagg atctgctctc
      781 cggctctcgg tggccatcgc ttgataggct cctcgcgttg tcgagatttc gcaacgagct
      841 gggtaaaaga atttatcgag gtgtataaac caataaagtt ttccaagacg gctgcaaaca
      901 agaagactcc gccgccgaca aacttttcct catcgaactc gatcgtgact tcgagtccgc
      961 ggcagaaccc attccacggc atcgagctcg gccggccgac tacctgttta ctggtcaagc
     1021 gcgtgatgcc gttgatttgc tggcgtacag acgaggaatc ggcaaagtca taaatcttta
     1081 gaatttcccg taatgcttcg agacctgcct cacaaaggga taaataattg aggccgagat
     1141 gcgagatgat tcgccattgt gttcctcggc gtagcggcgc gcgcagcgcc tcggtcggct
     1201 tacgcagaca acgtatgcgc gatagcggcg ccgccccctc gagctcgaac tcaccgcgat
     1261 cgcctccgaa cggcaatttt ccgggaagat cccgattgct gcatgtggtg tgaatgatca
     1321 aagtgtctac cgcggggagt gtaggccgaa aattgagatc cacgaacgac agatgcacgt
     1381 cgctgccgtt atcgcccttt tttggagatg gcttgcgcgc ggcataccag aaggcctgct
     1441 gttgctctcg ttcaagaccg tgcttgaatg agtagaacgg ctcgaattcg actacctcat
     1501 cagaaccggg agaaacactg gtgaccgaat cgatcgaata aacctcggtg gcgttctgtc
     1561 ttcgaatatc gggaaacacc tgatactcgg tgcttgtgtg gcttaggcgg acgggctcgg
     1621 ccgtttgcga aaacaagttg acaatcggcg tgcaacccag ccggaaagtg tctcgcgtaa
     1681 ccggctgctc caagcgcggc atccgattga gcaaaagccg aatttccatc cgatcgtcag
     1741 atcgatcgtg caccgcccga tccagcccgg tgagctcaaa gaataaaaac ttctccggga
     1801 aactaaaata ctcttgcagt agacgataac caagaaacga gcgcgcgccg taaggcagaa
     1861 ttccttcgtc ggcggcaaat ccgaccggac gcacgctgct caccggcaac gaaatatccc
     1921 tggcgctttc gttcggtttg ttggatggga tgcgcacttc cattacgttg ttgaagagca
     1981 gttcgtacag cgcgtaagtc agcgcgcctt cgccgtggag aaaaaaccga aaccgatcga
     2041 gctttaactg gacaaacttg ataccgctca ggcagcgaat ctcgagccgt atccatccga
     2101 gtgccgcttt cggaaaaaca cccaagcgtg agtccaccgg ttccaggctc gctgcctcga
     2161 tctgaaccgg ccacaaggtg acgggataac aggtgcggaa cctgcatggc gctccatcga
     2221 caggctgcga ataaagagtg gattgtttcg gaatggcgta tccgctggtg agctttcctt
     2281 gatccggatc tagaacaaac tgggccactg acatcgaggg aatcggcgcc agatagtgag
     2341 gataaagcac gttgagtaga gattctgtca cctccgggaa atcatcgtcg atcttgtggt
     2401 ggacccgcgc ggccagaaag gcaaatgcct gaatcaggcg ctcgacatgg ggatcttccg
     2461 aagcgtcgcc ttcaaggagc aagcgcgccg cgatcttagg atatttggca gcgaactcgg
     2521 ctcccatttg ccgaagaaag ctgagttccc tctcatagta ggtaataagg tcgtccggca
     2581 tcactgctcc cgctgaatca cgtactgttg agtgttcatc tgcaatactg cgtcgaaggt
     2641 cacaggctcc ggggacggat cgactcgtaa aaaagcgtcg atgcgaaagc gaagaccgcg
     2701 atcgtgctcc cgcggagctt cgagagttac ccgcactcgc aacagccgcg gctcaaacgt
     2761 gttgatggcg tcttcgactg atcgcctgat ccgaagccga tcgtcctgag ttagtaagct
     2821 gaatgaggta aaatccggca gcccatacgt caacaacgag cgggctactt ctttgaactc
     2881 gctgggcact tccttcaagg tttcctgccg tgaattgaga agcgcttcga gatcacgagc
     2941 gaccgatcgt tccaaagatc gaaaaccctt gatccggctc gtcgccggct cggtggagac
     3001 ttccggctca tcgtcgagca accggtcgag gatcgacgga acgatgttca cgtcgttatc
     3061 aattctagcc atacgcaaaa atagttcggg cggaatccag ctctaccgca aaaatcaaaa
     3121 acttgtcaac aaatgtgaac gatgggctcg cttggcacag tcgtcgtctg caatcgccat
     3181 gcggtcctgc aagctcaaac gtttatggtc tttgccagat gtcccagtca gcgggcattg
     3241 taataaacgg atgatacttt cagtttgcaa ctgaggatct aagcaaccag caggcccatt
     3301 tcggccagct cagcaagttt tcgttccaat tcgatcgcaa ggaagcagcg cgtctcggcg
     3361 ttgtcggtgc tcgcgccgtt ctttatttcc acggctgcgt ctccggattt tgtcgagact
     3421 tctaggccat cgagcaaggc agtacggtct ctgctgccgt ctaacagctg cagcagccgc
     3481 cggcttaagg gatcttcgac ccggaggttg aagtgacgca ggttcacaac ctccacgccc
     3541 tcaaggctct gcagacgcgc caaagcgctt gccactggtt ttaaacccgc acttgtcacg
     3601 acgttaggcg cagcaagact gaattcgaca atgccaccgg cgtaagctcg caacagcact
     3661 tcgccgagcg ttcgcgcatc atcgttagcg ccggccgtgg cgcatgaaga cgcgctctcg
     3721 catgatccac gcgcttgcgc gagcagctcc tgaaaatgga tcgctttcgg ccaggctttg
     3781 cccaacgccg acatggctgc tttaatcagt ggattatcgg tttgcagtga tgagttcttg
     3841 ggaccggaga acgtcaactg atcacgggag tcgagacaag cgttcgcgtc agcgggacgg
     3901 atcggcgagg cgatgtaaaa tgtagtcacc ttctctggcc gtggagtatc gtccagctcc
     3961 tggtcatcat ggcacaacaa ggtctgccgg aagcggcgac acttgaggaa atcaagatat
     4021 tgctcctttg caatcgcgct atcggagatc cggtttagta cttctactac tctggcgggg
     4081 aagatacctg tctgtatctc gaagaagtgc gcctcagaca gatacttcaa gccatgacgc
     4141 cctgcgtgtt caacgaactg ataaaaataa accggcgcgt tgtactcgga taagtcgtca
     4201 tgaaaaagcg agctgtcggc aaactctgta atccgatcca gctctttttg cagaaccatg
     4261 cgatagagat cgggctcgct gccggcatct atgatgaatt tgatcagcga gcgagcctgg
     4321 gtgacgcgct gctgcggatc gttaaactcg cttacgtgaa agcgcatcat ctcgcgtgcc
     4381 atatcccgca tgtggccgcc cggataggtg ttgtagctca cgtaagccac tccattgtcg
     4441 gccaggttct gctt
//
LOCUS       DASUVS010000228         8274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1621685, whole genome shotgun sequence.
ACCESSION   DASUVS010000228 DASUVS010000000
VERSION     DASUVS010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621685"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="VF182_12640"
     CDS             complement(<1..1493)
                     /locus_tag="VF182_12640"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX6437976.1"
                     /translation="MKSSASDRLACVDLPAFALQLLFKRHPEWRAYPAAVVAEDKPQG
                     LILQINERARRCGVLPGLRYAAAFSLARGLRAGEVRPDEIHKAIITLTQRLMRFTPEV
                     EPSAPEPGVFWLNGKGLQRLYTSPQEWGGAIHQDIKAQGFQANLVAGFSRFGTYAIAK
                     EKSGVTIFDDPAEERSAARKVPLDRLDIEAKFRDTLFKLGIKTVGALLSLPSAGLRER
                     FGAGAHRLYRMAAGDLWTPLDPCAPEEIVAQRHLLDDPENDVTRLLFLIKQLLHPMLA
                     TLAARSQALSALWLGFLIDKSEWLKEQIRPAVPTLEAAQILDLVRLRLESVKFPAGVI
                     EIELTAEACVASVEQLRLFNEQPKRDFDSANRALARLRAEFGAEAVVQAKLKDGHLPE
                     ARFTWEPLDQVKLSKNVLNEAKRLNSFNELNLLAPSMLVRRINEKPIMLAGGPYNRHE
                     DGWLLLGPKHGSIDKLTGPYVYSGGWWNREIQRDYYFAETRRGDLLWL"
     gene            complement(1456..1932)
                     /locus_tag="VF182_12645"
     CDS             complement(1456..1932)
                     /locus_tag="VF182_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase A"
                     /protein_id="HEX6437977.1"
                     /translation="MAFGLVREAQQRGEPVGWVTSMASCFYPPDAAQNGTDLAALVVI
                     RIPAAEKIARAGEKLLRSGGFGLLIFDLGKADIPMPLQSRLTGLAHHHQTALVCLTEK
                     QLKSFSLGALVSLRVHAEKKRASENRFACMLKVLKDKRCGPTWNHEELRFGPAGLC"
     gene            complement(2219..2827)
                     /gene="lexA"
                     /locus_tag="VF182_12650"
     CDS             complement(2219..2827)
                     /gene="lexA"
                     /locus_tag="VF182_12650"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX6437978.1"
                     /translation="MLPALTPRQKAIYEFLLKAIREKGYAPSIHEIGSRFQIASTNGV
                     SDHLKALEKKGYIRRIGKRAIEVLSALGKPVLTATREIQVLGTVPAGKPFFSEENSEG
                     LLTIPTDMGAGKLFALQVKGDSMIDAGILDGDRVIVKQQGTAENGEIVCAIIDGEATL
                     KRFYQEDGVITLRAENEKYAPITVSAGEFRIAGRVVGLLRKF"
     gene            complement(3045..4637)
                     /locus_tag="VF182_12655"
     CDS             complement(3045..4637)
                     /locus_tag="VF182_12655"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6437979.1"
                     /translation="MVHIRYSRWDITRRDSLDSDDVFDQLNENMNDTGDLQQAMRRLM
                     QKGIKQGDKQTKGVDDLLSQVAKEMRKLYDQYRLQSAMDEVQEQLEEVLEQEQQTLED
                     LDQSGQDVKANQQFLNDLPSKTSEAVEKLTSYSFQNPDAEKQFEQLLAQLDQIRKLEN
                     WLRREGSLFRGQTPMQFQQSQELMERMEDLRRLEGQLSSMQLKDVDMELLEKLLGGEP
                     KQDFEGVMRMQSLLEEGGYVLEQGDRFELTPRGIRRVGQLALRDIYQQLRRDGMGRHA
                     TRNRGSQELMTENSRPYVQGDPLHINMIHTLKNALLHGGGVPVRIQPGDFAVYESEHS
                     TRAATVLLLDMSWSMSWEGRFAAAKKVALAMETLVRSMHPRDYFGIVGFFTRAVELKA
                     KDLPQATWNMGDPFTNLQDGLHLASQLLERKPSVNQQMIVITDGQPTAYCRQGRLYCE
                     WPLSFGGISQRAAEETLKEAERITRKGITINTFMLDDSPVLRGFVDDLTRINKGRAFY
                     TRPDRLGEYLLVDYLSHQRKKV"
     gene            complement(4672..6069)
                     /locus_tag="VF182_12660"
     CDS             complement(4672..6069)
                     /locus_tag="VF182_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEX6437980.1"
                     /translation="MNDAKTIGELKKSGVAVLPIREEMRKNLVHCLETGKRILPGIVG
                     YDETVIPEIENAILSGHHMVFLGERGQGKSRVIRGLMNLLDERIPIIQGCEINDNPYH
                     PICRSCRKKLQELGDNTPIQWIDRDSRYGEKLATPDVSIADLVGEIDPIKVAEGRYLA
                     DEETIHYGLVPRTNRGIFAINELPDLTEKVQVGLFNLMEEKDVQIKGYKIRLPLDVVI
                     VASANPEDYTSRGRIITPLKDRFDVQIRTHYPKNIEEELAIMEQEAAPMDQRSRKVEV
                     PRFIKEILGQLTFEARKSNEINQSSGVSVRVTINNFESLISNAEKRALKCKENEIVPR
                     VSDLHAVMASMTGKIEMEYVGEDKKEDELVEKLLNRSILKIFDQYFSLNSLQKVVEYF
                     NSGWGVEVSDQMASHDYLEGIKEIPGLKEAVKTLGTQESPSLMASATEFVLEGLHLHQ
                     KLNKEIEGGRVTYGK"
     gene            complement(6161..6679)
                     /locus_tag="VF182_12665"
     CDS             complement(6161..6679)
                     /locus_tag="VF182_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX6437981.1"
                     /translation="MKQLWAPWRMVYIDQDSIETGCIFCNPNRGVEESRQALVLARTA
                     HSMVMLNKYPYNNGHLLLSPQQHENDLSKLSISEHADLSEALRRSVDIVRRVLKPSGI
                     NLGMNLGKCAGAGLADHLHWHIVPRWEGDTNFMPVIADTRVMPQHLFDSYDRLAPHFE
                     YFHREVSQNVRG"
     gene            complement(6676..6990)
                     /locus_tag="VF182_12670"
     CDS             complement(6676..6990)
                     /locus_tag="VF182_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEX6437982.1"
                     /translation="MTKRDLIDEVNKRFPHLSRRDSEVIINAIFDSMVEALAQGDRIE
                     IRGFGSFVVKHRRARQGRNPKSGVLVSVAAKRVPFFKVGKELRFRVDGKPFEEEEEEA
                     AT"
     gene            complement(7048..7947)
                     /gene="sppA"
                     /locus_tag="VF182_12675"
     CDS             complement(7048..7947)
                     /gene="sppA"
                     /locus_tag="VF182_12675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX6437983.1"
                     /translation="MANRHPILKGLGLLTGFVLVVFAAAFFYAYFTGGDAKALSLLAG
                     DGIGILQVDGTIDDSRDVLTELRRFRDAPWIKAIVLRIDSPGGAVAPTQEIFDELQKV
                     RSKKPLIASMGGMATSGGYYIASACEKIVANPGTMTGSIGVIMQLNNVEELMKKIGVK
                     GQNIKSGANKDIGSPFKALSPEGQAILQSLVDNVHGQFVSAVATGRGMEEAVVKKLAD
                     GRVYSGAQAKDLGLVDELGNLEYAVDLAAKRVGLEESPVLYYSRLEQERWWGRLFFSL
                     TGIQWSGRDRPGLRYEWSLSLLR"
     gene            complement(7966..>8274)
                     /locus_tag="VF182_12680"
     CDS             complement(7966..>8274)
                     /locus_tag="VF182_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX6437984.1"
                     /translation="DEGVEGLIHVSQLSSERVDKPSALYKVGDEVEAEVVNIDLTERK
                     IGLSIRALRKSEERQEMESYLTRDKQGGMFSFNTLLNEELRLDRDEEGQGRNKGKGNS
                     "
BASE COUNT         1805 a         2323 c         2135 g         2011 t
ORIGIN      
        1 agccatagga gatcgccgcg tcgggtttcg gcgaagtagt agtcgcgttg gatctcgcga
       61 ttccaccagc cgccggagta cacatagggg ccggtgagtt tgtcgatgct gccgtgcttg
      121 ggacctaaga ggagccagcc gtcctcgtgc ctgttgtagg gtccaccggc gagcatgatc
      181 ggtttttcgt taatgcggcg caccaacata ctgggagcga gcaagttcaa ttcgttgaaa
      241 ctgttcaatc gcttcgcttc attcaaaacg tttttggata atttaacttg gtcgagcggt
      301 tcccaggtga atcgagcttc ggggagatgt ccgtctttta gtttcgcctg gaccactgct
      361 tcggcgccga attcagcgcg caagcgcgcc agcgcgcgat tggccgaatc gaaatcgcgc
      421 ttcggttgct cgttgaacag ccggagttgc tcgacgctgg caacacaggc ctcggctgtt
      481 aattcgattt caatcacgcc ggcgggaaat tttaccgact ccaagcgcag gcgcaccaga
      541 tcgagtatct gagccgcttc gagtgtgggc acggcggggc gaatttgctc tttgagccat
      601 tcgctcttat cgatcagaaa accaagccat agcgcgctga gcgcctggct gcgcgcggca
      661 agcgtcgcca gcattgggtg aagtaactgt ttgatgagga agagcagccg ggtaacatcg
      721 ttttcaggat catcgagcag atgtctttgc gcaacgattt cttccggcgc gcatggatcg
      781 agcggagtcc agagatcgcc ggcggccatc cggtagagac gatgagcccc cgcaccgaaa
      841 cgttcgcgca aacctgccga tggaagcgaa agcaaagcgc caaccgtttt gatgccgagc
      901 ttgaaaaggg tatcgcgaaa cttagcttcg atatccagac gatccagcgg cactttgcgc
      961 gccgcactgc gctcctcggc gggatcgtcg aaaatcgtta cccccgattt ttctttagcg
     1021 atcgcataag tgccgaaacg gctaaagccg gcaaccaggt tagcctggaa tccttgcgct
     1081 ttaatatctt gatgaatagc gccgccccat tcttgcggcg atgtatagag gcgctgcaga
     1141 cctttgccgt ttaaccagaa cactcccggc tccggcgccg agggttcgac ctcgggcgtg
     1201 aagcgcatca gccgttgggt caaggtaatg atcgctttgt gaatttcatc cggccgaacc
     1261 tcgccggcgc gcaacccgcg cgctaacgaa aaagccgcgg catagcgcaa tccgggaagc
     1321 acgccgcagc gccgggctct ctcattaatc tgcagaatca atccttgcgg tttgtcctcg
     1381 gccaccacag ccgccggata agcgcgccac tcgggatggc gcttgaacag cagctgcagc
     1441 gcaaatgccg gcagatcaac acaggccagc cggtccgaag cggagctctt catgattcca
     1501 agtcggtcca caacgtttgt ctttgaggac cttgagcatg caggcaaagc gattttcgga
     1561 agcgcgcttt ttttcggcgt ggacccgtag cgacaccagc gcccccagag aaaacgactt
     1621 gagttgcttt tcggtgagac aaaccagcgc cgtctgatga tgatgggcga gcccggtcaa
     1681 acggctctgc aacggcatgg gaatatcggc cttaccgaga tcgaagatca gcaaaccaaa
     1741 gccgccggag cgcaacaact tctcgccagc gcgggcaatt ttttccgcag ccggtatacg
     1801 gatcaccacc agcgcggcca aatccgtacc gttttgagcg gcgtcgggag gataaaagca
     1861 actggccatc gaagtcaccc agcccaccgg ttcgccgcgc tgctgcgctt cccgcaccaa
     1921 gccaaatgcc atactcaacg ccgccgaaga cgccgaagcg gaaatttcaa ccaatcgtcc
     1981 agcgacctcg gtgagcgacc aaggcgtcga atggtgagtt aacggacgaa aatttttagc
     2041 ggaggaaagc attaaaataa tcgttcaata gttcagcgat tcaacggttc aatgtcagaa
     2101 ccgaatcggt cccggcgtat tgtttgaagt aaaaccttta cttcatctac ctttgattcg
     2161 agctaaagat cgtcgttctt acgttgaacg tctgaacttt tgaacgttga acaactcttt
     2221 agaattttct aagtagcccg accacacgcc cggcgatccg aaattctcct gcagaaacgg
     2281 tgatcggcgc atacttttca ttttctgccc taagcgtaat aacgccgtct tcttgataaa
     2341 accgctttaa cgttgcttcg ccatctatta tggcgcaaac gatttcgccg ttttcggctg
     2401 ttccctgctg tttgacgatc actcggtcgc catcgagaat gccggcatcg atcatgctgt
     2461 cgcccttaac ctgaagcgcg aaaagctttc cggctcccat atccgtcggt atggttaaaa
     2521 gtccttcgga gttttcttcg ctgaaaaatg gttttcctgc tgggaccgtg ccgagaacct
     2581 ggatctcgcg agtggcggtt agaacgggtt tccccaaagc cgacaagacc tcgatggccc
     2641 gtttaccgat gcggcggatg tagccttttt tttcaagagc tttgaggtga tcggagactc
     2701 cattggttga ggcgatctgg aaccgactgc cgatctcgtg gatcgaagga gcatagccct
     2761 tctctcggat ggctttgagc aagaattcgt aaatcgcctt ttgccggggt gtcagagcgg
     2821 gcagcattag gtagttatac tgaaagtatt ttcagtagtc aaggacgcgg cttagccgcg
     2881 ggttcaatcg ttcaagccgt tcaaaacgtt caaacgtttg gtcggtttct gagagaaaag
     2941 tctgtatgga ctaaaccggg caatcgttcc gaaaacgttt tgaacggagc gtagcggttg
     3001 aaacgttttg aacgattttg aaccggtgta acgcgcggag cgagttacac tttctttctt
     3061 tgatgcgaga gataatcgac caggaggtat tctccgagac gatcggggcg ggtgtaaaaa
     3121 gcgcggcctt tgttgatacg ggtgaggtcg tcgacgaagc cgcgcagcac ggggctgtca
     3181 tccaacatga aggtattgat ggtgatgcct ttgcgcgtga tgcgctcggc ctccttcaag
     3241 gtctcttccg cggcgcgttg actgatgccg ccgaaactca acggccattc acaataaagc
     3301 cgcccttgac ggcaataggc ggtcggttgc ccgtcggtga taacgatcat ctgctgattg
     3361 acgctgggct ttcgttccaa gagttgcgat gccaaatgca atccatcctg aagattggta
     3421 aacggatcgc ccatattcca agtcgcttgc ggcaagtcct tggctttcaa ttcgacggcg
     3481 cgggtaaaaa aaccgacaat gccgaagtaa tcccgcggat gcatcgaacg cacgagggtt
     3541 tccatcgcca gggcgacttt ttttgctgcg gcaaaacgcc cttcccaact catcgaccag
     3601 ctcatgtcga ggagcaacac cgtggcggcc cgggtcgagt gctccgactc gtacacggca
     3661 aaatctcccg gttgaattct taccggaaca ccgccgccgt gcagcaaggc gtttttcagc
     3721 gtatgaatca tgttgatgtg aagcgggtct ccctgcacgt aggggcggct attttcggtc
     3781 atcaattcct ggctgccacg attgcgggta gcgtgccggc ccatgccatc gcggcgcagc
     3841 tgctgataaa tatctctcag cgctaactgc ccgacgcgcc ggatgccgcg cggggttaat
     3901 tcaaagcgat ctccttgctc gagaacatac ccgccctcct cgagcaaaga ttgcatgcgc
     3961 atcacgccct cgaaatcctg cttgggttcg ccgccgagaa gcttctccaa aagctccatg
     4021 tcgacatcct tgagctgcat gctcgatagt tgcccttcga ggcggcgcag gtcttccatg
     4081 cgctccatca actcctggga ctgctggaat tgcatcggcg tctggccgcg aaacaaactt
     4141 ccttcgcgcc gcaaccaatt ttcaagtttg cgaatctgat ccagctgggc gagtaattgt
     4201 tcgaattgtt tctcagcgtc cggattctga aaagaatagg aggttaattt ttccaccgcc
     4261 tcgctggttt tgctcggcag atcgttgaga aactgctgat tggccttgac gtcttgtccg
     4321 gactgatcga gatcttcgag agtctgctgc tcctgctcca ggacctcttc caattgctcc
     4381 tgaacttcgt ccatcgcgga ctgcaaccgg tactggtcat agagcttgcg catttccttg
     4441 gcgacctgag acaataaatc gtcgacgcct ttggtctgtt tgtctccctg cttgatgcct
     4501 ttctgcataa gccggcgcat cgcctgctgc agatcgccgg tatcgttcat gttctcattg
     4561 agctgatcga acacgtcgtc cgaatcgagg gagtcacgcc gggtaatatc ccaacggctg
     4621 tagcggatgt gcaccatggt tgattggctg ctttacggag tggtcaaaga tttacttgcc
     4681 atacgtgacc ctgccgcctt cgatctcttt gtttaacttt tgatgcaagt gtaggccttc
     4741 gaggacgaat tcggtggccg aggccatcag actgggcgat tcctgagttc caagggtttt
     4801 caccgcttcc ttcaatccgg gaatttcttt gatgccttcg agataatcgt gcgacgccat
     4861 ctgatcggag acttcaactc cccaaccgga gttaaaatac tcgaccactt tttgcagcga
     4921 attgagactg aagtattgat cgaatatttt taaaatggag cgattgagca gcttctcgac
     4981 cagctcgtct tccttcttat cctcgccaac atactccatc tcgattttgc cggtcattga
     5041 cgccatcacc gcatgtaaat cactgacccg cggcacgatt tcattctctt tgcatttgag
     5101 cgcccgtttc tcggcattgc tgatcaagct ttcgaaattg ttgatggtga cacgcacgct
     5161 gacgcccgaa gattgattga tctcgttcga tttgcgcgct tcaaacgtca gttggccaag
     5221 gatctctttg ataaagcgag gcacctcgac tttgcggctg cgctggtcca tgggagcggc
     5281 ttcctgctcc atgatcgcaa gctcttcctc gatgttcttg ggatagtgag tgcgaatctg
     5341 cacgtcgaag cggtccttaa gcggcgtgat aatgcgaccc cggctggtat aatcctccgg
     5401 attggcgctg gcgacgatca ccacatccaa cggcaggcgg attttataac ctttgatttg
     5461 aacgtctttt tcctccatca agttgaacaa acccacttgc actttttcgg taaggtcggg
     5521 caattcattg atcgcgaaaa tgccgcggtt ggtgcgcggc accaaaccgt agtgaatggt
     5581 ttcctcatcg gcaagataac gcccctcggc aactttgatg gggtcgattt cacccaccag
     5641 atcggcaatc gaaacatcgg gagttgccaa tttctcacca tatcggctgt cgcgatcgat
     5701 ccactgaatc ggtgtgttat cacccaactc ctggagtttt tttcggcagg aacggcaaat
     5761 cggatggtac ggattgtcgt tgatttcaca gccctgaatg atcgggatgc gttcatcgag
     5821 cagattcatc aagccgcgaa taacgcgcga cttgccctgt ccgcgttctc cgaggaagac
     5881 catgtggtga cccgacaaga tggcgttttc tatttcggga ataaccgtct catcatagcc
     5941 gacaatgccg ggcaaaatcc gttttccggt ttcgaggcaa tggacgagat tcttgcgcat
     6001 ctcctcgcga atcggcaaaa cggcgacacc gcttttcttg agctctccta tcgttttcgc
     6061 gtcattcacg ttgacccgcc tccctttaaa caattaatgt tgttggaacg cttcgaagca
     6121 cttgtgtaga ttggatgagc tgtccctata gcagcctaca ctacccgcgg acgttttggg
     6181 acacctcacg atgaaaatac tcaaaatgcg gcgccagacg atcgtaactg tcaaacaggt
     6241 gctgcggcat cacccgggtg tcggcaataa ccggcataaa gttggtgtca ccctcccagc
     6301 gcggcacgat atgccaatga agatgatccg cgagtcccgc accggcgcac tttcccagat
     6361 tcatgcccaa attaattccg gaaggtttca ggacccgacg aacgatgtcg acgctccgtc
     6421 ttagcgcttc actgagatcg gcgtgttccg agatcgagag cttcgacagg tcgttctcgt
     6481 gttgctgagg cgataaaaga agatggccgt tattataggg gtatttgttg agcatcacca
     6541 tgctgtgcgc cgtccgcgcc agaacaagcg cctggcggct ttcttcgacc ccgcggttcg
     6601 gattacaaaa gatgcagcct gtctcgatgg agtcctggtc gatataaacc attctccaag
     6661 gagcccacag ctgcttcatg tagccgcttc ctcttcttcc tcctcgaagg gttttccatc
     6721 aactctaaag cgcaattctt tgccaacttt gaagaacgga actcgtttcg ccgccaccga
     6781 taccaatacc ccgctcttgg gattgcgtcc ctggcgagcc cgccgatgct ttacgacaaa
     6841 actaccaaag ccgcggatct cgatccgatc accttgggcg agagcttcga ccatcgaatc
     6901 gaagatggcg ttgatgatga cttccgaatc tcgtctggag agatgtggaa atctcttatt
     6961 tacctcatct atcaaatccc ttttcgtcat aacgctcccc tcgcgacgta acaaaattct
     7021 tggctcgaaa agcagcggct gcaggactca gcgtagcaga gacaacgacc actcatatcg
     7081 aagtcctggt ctatcgcgcc cggaccactg aatacccgtt aaagagaaaa acaatctgcc
     7141 ccaccagcgt tcctgctcca gtcgtgagta gtaaaggacc gggctctctt ccaatccaac
     7201 ccgcttcgcc gccagatcca ctgcatactc caaattcccc aattcatcca ccaatccgag
     7261 gtctttcgcc tgagctccgg aatagacccg accatcggca agcttcttga cgacagcctc
     7321 ctccatgcct ctgcctgttg cgacggcgct aacaaactgg ccgtggacgt tgtcgaccag
     7381 cgactgcaaa atcgcttgtc cttcgggtga aagcgctttg aaaggcgagc caatatcctt
     7441 attggcgccg cttttaatat tttgtccctt taccccgatc ttcttcatca gctcttcgac
     7501 gttgttgagc tgcatgatga caccgattga gccggtcatg gttcccgggt tggcgacaat
     7561 tttctcacaa gcgctggcaa tgtagtaccc tccggaggtc gccattccac ccatcgatgc
     7621 aatcaatggt tttttgcttc gtaccttctg cagctcatcg aagatttcct gcgtcggcgc
     7681 cacggcgccg cccggcgaat cgatgcgcaa cacaatcgct ttgatccatg gggcgtcacg
     7741 aaatcgccgg agctccgtta gtacgtcgcg tgaatcgtcg atggtgccgt cgacctgtag
     7801 gatgccgata ccgtctccag caagaagcga caaagccttg gcatcgcctc ccgtgaaata
     7861 ggcgtaaaag aaggcggccg cgaagaccac cagtacaaat cccgtaagca ggcccaaacc
     7921 tttgaggatc ggatgccggt ttgccatttt gatgactgcg ctcctttatg agttgccctt
     7981 gcccttgttg cggccctgtc cctcttcatc acggtctagt ctcagctctt cattcagcaa
     8041 ggtattgaaa gagaacattc cgccttgttt gtcgcgggta agataacttt ccatctcctg
     8101 gcgctcttca ctcttgcgca aggcacggat gctcaggccg atctttcttt ccgtgagatc
     8161 gatgttaacg acttcggcct ccacctcgtc gccgacttta tacaaggctg aaggcttatc
     8221 gacgcgctcg ctgcttagct gcgatacgtg gatcaatcct tccacccctt catc
//
LOCUS       DASUVS010000229         6327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1402867, whole genome shotgun sequence.
ACCESSION   DASUVS010000229 DASUVS010000000
VERSION     DASUVS010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402867"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="VF182_12685"
     CDS             <1..1543
                     /locus_tag="VF182_12685"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX6437985.1"
                     /translation="ILYDDSNPLPAQLRREGVQFVGFPANKLVAKLVPASPLRAKQRN
                     MLYVGALAYLFGIDMEVIKSVLEDTFGKKPAVIESNLICIDAGYQYMKEKGFSQNIAR
                     LEVIPNGNKGKIITEGNTVAALGAIYGGASVICWYPITPSSSLAEAMEFYLPKLRKLK
                     DGKKAYAIVQAEDEIASAGMIAGAGWAGARAMTSTSGPGLSLMNETIGLAYYAEIPCV
                     FFIVQRGGPSTGLPTRTQQADISLMYGASHGDTRQMILIPHDMNSCFDFARQSFNYAD
                     RFQTPIFVAMDLDLGMNLWPSEPLKLQPESFDYGKRLSAEQLDEWSKTGKKFRRYFDQ
                     DGDGIPYRTVPGNQNRMASYFTRGSGHDADARYSEDETDYKYILDRLRKKFETARKFV
                     PKPIVEKERGVKTGIICYGSSYEPVREARDRLKAAGLKTNHLLIRALPLTSEVRDFLV
                     EHDTIYLVEQNRDGQMAAIIKDENPDLGSKLTSILVYNGLPVAAGEVVRQIFDAAQLA
                     QPKTA"
     gene            1577..2605
                     /locus_tag="VF182_12690"
     CDS             1577..2605
                     /locus_tag="VF182_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX6437986.1"
                     /translation="MAEAKLNRINLSEKDYEGAPSTMCRGCGHDAISASVIRAYYESS
                     IDPRKVVKLSGIGCSSKTPAYFLSQSFGFNAVHGRMSAVATGSNLANHTLMPIGVSGD
                     GDTSAIGLGNFCHMMRRNVNITYIIENNGVYGLTKGQFSATADVGSVMKGGKVNDIPA
                     IDLAELAITLGATYVARSFSGDRKQLTPLVQGSLHHKGSSILDVISPCVTFNNHEGST
                     KSLKYVKGHLDPIHDLDFIPPFENIEADYDEGTDHEVSLHDGSRIVLHKLGKDYDPTN
                     KAQAIQAIHAAIAEGKFLTGLIYYNPNRKEFAEELNLCDIPLSELTQADLQPPLEALD
                     QLNAEMMK"
     gene            complement(2679..3329)
                     /locus_tag="VF182_12695"
     CDS             complement(2679..3329)
                     /locus_tag="VF182_12695"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018930535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX6437987.1"
                     /translation="MEILKTLKDRCDPRYAALIVVDVQNDFVSPEGSAGKRGDDVGAA
                     MAMIPNLTSLIDQARKVGLTVVYIRTTHSDWTDTASWIYRTSQKSGLNTCREGTWGAE
                     FYNGIAPLPSERVVIKHRYSAFINTDLNTVLKARGIESILVCGVATNVCVETTARDGY
                     MYDYYVTMIDDCSAAYDAKLHMNTLENIRRHFGLVASSHQITETWSGLPVKQAVGL"
     gene            complement(3344..4333)
                     /locus_tag="VF182_12700"
     CDS             complement(3344..4333)
                     /locus_tag="VF182_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010494504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX6437988.1"
                     /translation="MLKVIPVEEAVGLPLAHDITEIVPGKHKGPAFRRGHVVRKEDIS
                     KLLDVGKRNLYVMELDRDELHEEDAAQRLAKAAAGINLKLVGPSEGRVNLVAEISGVL
                     KIDSDLLYRFNSLGNLIMATLQSDRYVNEGEVVAGTRTIPVVVKEELIQRAEALCKDR
                     PIVRILPMPPRRIHLIVTGSEVYTGRIQDGFEPVVRRKAAALGSELTDKQVANDDPDQ
                     IAKLIQEAQRTGAEIILVSGGMSVDPDDKTPEGIRRSGATIESHGFPVLPGSMFLMAY
                     LNGTPILGLSGCVLHDPFSAFDILLPRLLAGDKVTRMDIMAMGHGGLQKKHDH"
     gene            complement(4602..5612)
                     /gene="moaA"
                     /locus_tag="VF182_12705"
     CDS             complement(4602..5612)
                     /gene="moaA"
                     /locus_tag="VF182_12705"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013172840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX6437989.1"
                     /translation="MLVDALKRPIKDLRISVTDRCNFRCSYCMPLDEYEWINKKEILT
                     FEEISRLATLFVGLGIEKIRLTGGEPLVRQNLDKLVAKLSSITGLKDLCLTTNGALLA
                     EKVAALKAAGLKRVNISVDSLNPDKFRQMTKRGDLTKVLDGIFAAKRHGLNPIKLNAV
                     IEKGINEDDILSLVEFSRNNGFALRFIEYMDVGNSNDWTSGKLVSKKEMIETINSQYP
                     LKEVGRAGGSAPSVDYEFIDGRGDVGVIASVTEPFCSSCTRVRLTADGKIVTCLFSGI
                     GHDVKGLLRGGASDEEISKFIVSIWEERTDRYSAERLEALRSSNYDPKDHKKIEMISL
                     GG"
     gene            5668..>6327
                     /locus_tag="VF182_12710"
     CDS             5668..>6327
                     /locus_tag="VF182_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6437990.1"
                     /translation="MLPLGDLVSCAGMSLRQQQIAVFLGTIAIIAGLFAAGVWLIAQR
                     LTTEATLQTALLMARQVEIALADSLKQRPVVVQTGPSRTQSSSFWSFLGNIFPGSSRT
                     NTPQTIVRRPSRHEEVKGLMRAYVDRSGSIQAMWVLNADGKVLYSSAGQEKTDAYRDA
                     QFDEALRRGVTLINPRAQGKATFYDVVVPLQMPEGVHGPGGLRLWIDPADWTELLTGM
                     WR"
BASE COUNT         1546 a         1690 c         1598 g         1493 t
ORIGIN      
        1 gattctttat gacgattcca atccgctgcc cgcccaacta aggcgcgaag gcgtgcaatt
       61 cgtcggcttc ccggcgaata agcttgtcgc caaattggtg cccgcctcgc cattgcgcgc
      121 aaagcagcgc aatatgctct atgtcggcgc cttagcctac ctgtttggca ttgatatgga
      181 agttatcaaa tccgttctcg aggacacctt cggaaaaaaa cccgcggtga tcgaatctaa
      241 cctcatctgt atcgatgccg gctaccaata tatgaaagag aaaggattct cgcagaacat
      301 cgctcgacta gaggtgatac cgaacgggaa taaagggaaa attataaccg agggaaacac
      361 cgttgcagcg ttgggcgcga tctacggcgg cgcatcagtg atctgttggt atccgatcac
      421 tccgtctagc tcattggcag aagcaatgga gttctatctg ccgaaattgc gtaagctcaa
      481 ggacggtaaa aaagcttatg cgatcgtcca ggcggaagat gaaattgcct cggccggcat
      541 gatcgcagga gccggttggg ccggggcgcg cgcgatgaca tccacgtccg gacccggcct
      601 ttcgctcatg aatgagacca taggtctggc atattacgcc gaaattccat gcgtattctt
      661 tattgtgcag cgcggcgggc cgtcgaccgg attaccgaca cgaacacagc aagccgatat
      721 ctctttgatg tatggggcgt ctcatggtga tactcgccaa atgatcttga ttcctcacga
      781 catgaactcc tgtttcgatt ttgcccggca gagcttcaac tatgccgacc gatttcaaac
      841 accgattttc gtggccatgg atctagactt aggcatgaat ctctggccct ccgaaccgtt
      901 gaaactacag ccggaatcgt ttgattacgg taaacggctc agtgcggagc aactggacga
      961 atggtcaaaa accggtaaaa agtttcgtcg ctactttgat caagacggcg atggtatccc
     1021 gtacagaacg gttccgggga atcagaaccg gatggcatct tactttaccc gaggctctgg
     1081 ccacgacgcc gatgccagat attccgagga tgagactgat tacaagtata ttctcgaccg
     1141 tttgcggaag aagttcgaga cggccagaaa gtttgttccc aaaccaattg tcgaaaaaga
     1201 aagaggcgtt aaaactggca tcatctgtta tggttcgagc tacgaaccgg tgcgagaagc
     1261 ccgcgaccgg ctcaaggcag caggattgaa aaccaaccat ctgttaatcc gcgcgttacc
     1321 gctgacgagc gaagtcaggg attttctcgt tgaacatgac accatctacc ttgttgagca
     1381 gaaccgcgac ggtcaaatgg ccgcgatcat caaagatgaa aatccggatt taggatccaa
     1441 gctcacaagc attctcgttt ataatggctt gcccgtggca gctggcgaag ttgtcaggca
     1501 gatttttgac gccgctcagc tagcgcagcc aaaaaccgct taattcttaa ttttaaatct
     1561 tagtaaggac agagaaatgg cagaagcaaa actcaatcgt atcaacttgt cggaaaaaga
     1621 ttatgaaggc gcaccatcaa ccatgtgccg aggctgcggc catgacgcga tctcagcatc
     1681 ggttatacgc gcttattacg agagcagcat cgatccgcga aaagtggtca agttaagcgg
     1741 catcggttgt tcatccaaaa cgcctgcata ctttctaagt caatcgttcg gtttcaacgc
     1801 cgtacacggc agaatgtcag cggtggcgac cggatccaat ttggcaaacc acacgctcat
     1861 gccgattggg gtatccggcg atggcgatac gtcggcgatc ggtctaggaa atttttgtca
     1921 tatgatgcgg cgcaacgtga acattaccta cattatcgag aacaatggtg tgtacgggct
     1981 gactaaaggc cagttttccg cgaccgctga tgtcggatcg gtaatgaagg gcggcaaagt
     2041 caatgacata ccggcgatcg atctagccga attggcaatc accctcggag ccacctacgt
     2101 cgcccggagt ttctccggag accgcaagca gttaacccct ttggtccaag gctctctaca
     2161 tcacaaagga agctctatac tggacgtcat cagcccgtgc gtcacgttta acaaccacga
     2221 agggtcgacc aaaagcctca aatatgtcaa aggtcacctc gatcccattc acgacctgga
     2281 cttcattcct ccgtttgaga atatcgaagc tgactatgat gaaggcactg atcacgaagt
     2341 gtctttgcac gacggctcgc ggattgtctt gcataagttg ggcaaagact atgacccaac
     2401 caacaaagcg caagcaatac aagcgatcca tgcagccatc gccgaaggaa aattcttgac
     2461 tggtttgatt tactacaacc cgaatcggaa ggaatttgct gaggaattga acctctgcga
     2521 cataccctta tcagagctaa cccaggcgga ccttcagcct ccgctagaag cgttagatca
     2581 gctcaatgca gaaatgatga aataaccccg cgcgatgccc agcgaacaag agcctggccg
     2641 gttaatccgc gccaggctct tttgcatgcg tcccatcact ataagcccac ggcttgtttt
     2701 acgggcagtc cactccaggt ttcggtaatt tggtgtgacg aagcgacgag gccaaaatgg
     2761 cgacgaatgt tctctaacgt attcatgtgg agcttggcgt cataagccgc ggagcagtcg
     2821 tcgatcatcg tcacgtaata gtcatacata taaccatctc gcgcggtggt ttcgacacag
     2881 acattggttg cgacgccgca aaccagaatg ctttcaatac cacgggcttt caacacggtg
     2941 ttcagatccg tattgataaa ggcgctgtag cggtgcttga tcacgactcg ctcactcgga
     3001 agcggcgcaa tcccattata aaactccgcg ccccaggtgc cctcacggca agtattcagc
     3061 ccgcttttct gcgatgtgcg ataaatccag gacgcggtgt cggtccaatc gctgtgtgtg
     3121 gttcttatat aaacgaccgt aagccctact tttcgagctt gatcgataag actcgtcagg
     3181 ttcggtatca tagccattgc cgcaccgacg tcgtcgccgc gcttgccagc gcttccttct
     3241 gggctaacga aatcattttg gacatcgacc acaatcaacg ctgcatagcg cggatcgcaa
     3301 cggtctttta gcgtttttag aatctccatc gtttcctccg ttcttagtga tcgtgcttct
     3361 tctgcaaccc gccgtggccc atggccatga tatccatccg ggttacctta tctcccgcca
     3421 aaaggcgcgg gaggaggata tcaaaagcgc tgaacggatc atgcaggaca cagccggaca
     3481 atcccaaaat cggcgtaccg tttaaataag ccattagaaa catcgaacct ggaagcacgg
     3541 gaaagccgtg agattctatt gtcgcgccgc tgcggcgaat cccttctggt gttttgtcgt
     3601 cgggatcaac cgacattcca ccactgacga gaatgatctc tgcaccggtt cgttgggcct
     3661 cttggattag cttggctatt tgatccggat cgtcattggc aacttgcttg tcggttagtt
     3721 cggatcccaa tgcggcagcc ttccgccgca cgacaggctc aaagccgtct tggattctgc
     3781 ccgtgtaaac ttcgctgccg gtaacgatca aatgaatgcg ccgcggcggc atcggcaaaa
     3841 ttcgcacgat cggacgatct ttacataaag cctcagcacg ctgaatcaac tcttctttca
     3901 ccaccactgg gatcgttcgt gtaccggcta ccacctcgcc ctcattgaca taccgatctg
     3961 attgcaatgt tgccatgatc agattgccca atgagttgaa acgatagagc agatcagagt
     4021 cgattttcaa cactccggaa atctccgcga ccaagtttac tctgccttca ctcggaccga
     4081 ccagtttgag attgataccg gcggccgcct tggccaagcg ttgcgcggca tcttcttcat
     4141 gcagctcgtc tctatcaagc tccataacgt agaggttgcg cttaccgaca tcgagcagtt
     4201 tcgagatatc ttctttacgg accacgtgac cgcgccgaaa cgccgggccc ttgtgctttc
     4261 ccggtacgat ctcggtgata tcgtgggcca gtggaagtcc caccgcttct tcaactggta
     4321 taactttgag cattatcgat ctccttcaaa acaagcgtaa gcccttgatg tacgggcaat
     4381 tcttccacgg ggcatcctct ttgtccagcc cgagttgggt gcgaaatgcg cggacgatta
     4441 cctctatcct acggccgctg ttaccgccgc caggtgtccg tgcccaacct ggcgctacga
     4501 cttcacttgg agaaaggaaa gagagtcagc aacagactca ggtgcgagtt ggaacgcgag
     4561 cggagagcgg gacatgctcc ttgtcaggta aactgattgg gttaaccgcc taaagagatc
     4621 atctcgattt tcttatggtc cttaggatcg tagttggatg agcgaagagc ttccagccgt
     4681 tcggccgaat agcggtcggt ccgctcctcc cagatcgaga caataaactt gctaatttcc
     4741 tcgtccgatg cgccgccgcg taacaaccct ttgacatcgt gaccgatacc agaaaatagg
     4801 caggtgacaa ttttcccatc cgccgtaagt cgcacacgag tgcagctcga gcagaacggc
     4861 tcggtgacgg aagcaatcac gccaacgtcg ccgcgcccgt cgataaactc atagtctacc
     4921 gaaggtgcgc ttcctcctgc ccgcccaact tccttaaggg ggtattgaga gtttatcgtc
     4981 tcaatcatct cctttttaga aacgagtttt ccggaagtcc aatcgttgga attgccaacg
     5041 tccatgtatt cgatgaaacg gagggcaaag ccgttgttac gtgagaattc gaccaagctc
     5101 aagatatcat cttcatttat gcctttttca atcaccgcat tcagtttgat cggattaagg
     5161 ccatgacgct tagcggcaaa gatgccatct aagaccttgg tcaagtcacc tcgcttagtc
     5221 atttgtctga atttatccgg gttcaacgag tcaacgctga tgtttacgcg ctttagcccc
     5281 gctgctttaa gcgctgccac tttttcggca agcaaagcgc cattggtagt caggcacagg
     5341 tcttttaggc cggtaatgga ggaaagcttg gccacaagct tgtcaagatt ttgcctgacc
     5401 aggggttcgc cacccgtgag acgaatcttc tcgatgccga gtccgacaaa tagggtagcc
     5461 aaccgcgaga tttcttcgaa cgttagaatc tccttcttgt tgatccattc gtattcatcc
     5521 agcggcatgc aataactgca acgaaaattg cacctgtccg ttaccgatat tctaagatcc
     5581 ttgattggcc gctttaaagc atctaccagc ataagataac tatgcagcaa aggtcttgga
     5641 ggcgcaagtt acgtccgcag ttgaactttg ctgcctctgg gcgatctggt atcctgcgcc
     5701 ggcatgagtt tgcggcagca gcaaatagcc gttttccttg gcaccatcgc tatcatagcg
     5761 ggtctttttg ccgcgggtgt ctggttgatt gcgcaacgcc tgactactga ggctacccta
     5821 caaacggcac tcttgatggc gcggcaggtg gaaattgcct tggctgatag cctgaaacag
     5881 cggccggtcg tcgtgcaaac cgggccgtcg cggacccagt cttcctcatt ttggagtttt
     5941 cttggaaata ttttccccgg cagcagccgc acaaacacgc cccaaacgat cgtccgcaga
     6001 ccatcacgcc atgaggaagt gaaaggactc atgcgcgcgt atgtcgatcg cagcggcagt
     6061 attcaagcga tgtgggttct aaatgccgac ggaaaggtgc tttatagctc ggcggggcag
     6121 gaaaaaacag acgcctatcg agatgcgcaa ttcgatgaag cactgcgccg cggtgttact
     6181 ctgatcaatc cacgagctca aggcaaagca acgttttatg atgttgtggt accattgcaa
     6241 atgccggagg gggtgcatgg cccaggcgga ctgcgcttat ggatcgatcc ggccgactgg
     6301 actgagcttt tgaccggcat gtggcga
//
LOCUS       DASUVS010000230         2646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1403023, whole genome shotgun sequence.
ACCESSION   DASUVS010000230 DASUVS010000000
VERSION     DASUVS010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403023"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2355)
                     /gene="tssH"
                     /locus_tag="VF182_12715"
     CDS             complement(<1..2355)
                     /gene="tssH"
                     /locus_tag="VF182_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HEX6437991.1"
                     /translation="MNVNLKSLIGKLNATGRQALEAAAGLCVTRTHYDIDIEHLLLKL
                     VEAENTDIQKILRHYEVDQARLSRDLTRGLDRLKSGNARTPAFSPRIPRLITEAWNLA
                     SIDFGATKVRSGHLLLAILGDEDLRRLAREVSPELQKVSVEELHKHFADLTAATAEDD
                     MTSPAGTESAEADMRGPTKTPSLDQYTIDLTDRARKGQIDPVLGRDFEIRQMVDILTR
                     RRQNNPILTGEAGVGKTAVVEGLALRIAQGDVPPPLKNVALKSLDLTLLQAGAGVKGE
                     FENRLKSVIEEVKASAQPIILFIDEAHTMIGAGGQAGQGDAANILKPALARGELRTIA
                     ATTWSEYKKYFEKDAALTRRFQVVKVDEPTEEQAVAMLRGLMSTLESHHNVRILDEAM
                     DAAVRLSHRYITGRQLPDKAVSVLDTSSARVALGQMTTPPALEDCQRRITQLDTEIGI
                     LDREALTGGNTNERIDALAKEKAQIESRLAELNTRWNEEKRRVEELRQLRQKLEAHAA
                     ASKQPENGNTDAKLSPEDVKQVQGEIARLNDELGKIQGESPLMQVCVDAQAVAEVVSG
                     WTGIPVGKVMKDEIQTVLSLKDRLEERIIGQSHALEAIAQRIRTARAKLEDPRRPIGV
                     FMLVGPSGVGKTETALALADTLFGGERNVVVINMSEYQEGHTVSGLKGSPPGYVGYGE
                     GGVLTEAVRRKPYSVVLLDEMEKAHTDVMELFYQVFDKGMLEDGEGREIDFKNTLILM
                     TSNAGTDTIAKLCADPDTKPEPDVLADALRPDLLKAFKPAFLGRA"
     gene            complement(2370..>2646)
                     /locus_tag="VF182_12720"
     CDS             complement(2370..>2646)
                     /locus_tag="VF182_12720"
                     /inference="COORDINATES: protein motif:HMM:NF018675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEX6437992.1"
                     /translation="RGFLPSENGFSSLVELTRFFVGLTLDFDVQLVLQAAEAPGCRLN
                     KPGPGALRLGWSSWLKTKEFSCDAAEAVLGRHLTRLQKLSGHPSQAT"
BASE COUNT          510 a          805 c          722 g          609 t
ORIGIN      
        1 ggctcggccc aggaacgccg gcttgaacgc tttgagcagg tcgggacgca gcgcatcggc
       61 gagcacgtcc ggttccggct tggtatccgg atcggcgcag agtttcgcaa tcgtgtcagt
      121 gccggcattg gaggtcatga gaatcaacgt attcttgaaa tcgatttctc taccttcgcc
      181 gtcctccaac atgcccttgt cgaaaacctg atagaaaagc tccatcacat cggtgtgcgc
      241 tttttccatt tcatcgagaa ggacgaccga atacggtttg cgtctgaccg cttccgtgag
      301 cacgccgccc tcgccgtaac cgacatatcc cggcggcgag cccttgagac ccgagaccgt
      361 atgcccttcc tgatattcgg acatgttgat gacgacgaca ttgcgctcgc cgccaaatag
      421 ggtgtcagcc agcgccaacg ccgtttccgt ttttcccacg ccgctcggtc ccaccagcat
      481 gaaaacgcct atgggccggc gcggatcttc cagcttggcg cgcgcggtac gaatccgctg
      541 ggcaatcgct tcgagcgcat gggactggcc gatgatccgc tcttccaaac ggtccttgag
      601 cgagagcacc gtctggatct cgtctttcat gactttgccg acgggaattc cggtccagcc
      661 ggagaccact tccgcaacgg cctgggcatc cacgcaaact tgcataagcg ggctctcgcc
      721 ctgaatcttg cccagctcat cgttgagccg cgcgatctca ccctgcacct gcttgacgtc
      781 ctcgggagac agcttcgcgt ctgtgttgcc attctccggt tgtttgcttg cggcggcatg
      841 ggcctcgagt ttctgcctga gttggcgcaa ctcttcgaca cgccgttttt cctcgttcca
      901 gcgggtgttg agctccgcta aacgactttc gatctgagcc ttctctttag ccagggcgtc
      961 gatacgttcg tttgtgtttc cgccggtgag agcttcccgg tcgagaattc cgatctcagt
     1021 gtcaagctgg gtgatgcggc gctgacagtc ttcaagcgcc ggcggcgtcg tcatctggcc
     1081 cagagcgact cgcgccgagc tggtatcgag cacactgacc gccttgtcag gcaactgcct
     1141 gccggtgatg taacgatgtg aaagacgcac ggcggcatcc atcgcctcat cgagaatccg
     1201 cacgttgtga tgactttcga gcgtgctcat cagaccacgc agcatcgcca ctgcctgctc
     1261 ctcggtaggc tcatcgactt tcacgacctg aaatcgccta gtgagagccg cgtccttctc
     1321 gaaatatttt ttgtattcag accaggtcgt cgccgcgatc gtgcgcagtt cgcctcttgc
     1381 cagcgccggt ttcagaatgt tggcggcatc accctgcccc gcctgcccgc cggcgccaat
     1441 catggtatgc gcttcatcga taaaaagaat gatcggctgc gccgatgctt tcacttcctc
     1501 gatgacagat ttcaggcgat tttcgaattc acctttgacc cctgcacctg cctgcaacag
     1561 agtgagatcc agagacttaa gtgcgacatt tttaagcggc ggcggtacgt cgccttgcgc
     1621 aatccgcagc gccaggcctt cgactaccgc tgtttttccc acgcccgctt cgcccgtcag
     1681 gatgggattg ttctgtcgcc gtcgcgtaag aatatcaacc atctgccgga tctcgaaatc
     1741 gcggccaagc accggatcga tttgtccctt gcgagcccgg tcggtcaaat cgatggtata
     1801 ctgatcgagc gaaggcgttt tcgtcggtcc gcgcatgtcc gcctctgcgc tctcagtacc
     1861 tgccggagag gtcatgtcat cctcggctgt tgctgccgtg aggtcggcga aatgcttgtg
     1921 cagctcttca accgagacct tctgcagctc gggtgacact tcccgcgcca ggcggcggag
     1981 atcttcatcg ccgaggatcg ccaggagcag atgaccggag cgaaccttag tcgcgccaaa
     2041 atcaatcgag gctaaattcc acgcctcggt gatcaaccgc ggaattctcg gcgagaacgc
     2101 cggtgtgcgc gcgttgccgc tcttcagccg gtcgagccca cgcgtgagat cgcgcgagag
     2161 tctggcttga tcgacttcat agtgtcgaag aattttctgg atgtcggtat tctcggcttc
     2221 gacgagcttg agcaacagat gctcgatatc gatatcataa tgagtgcgag tcacgcacaa
     2281 acctgcggcg gcttcgaggg cctggcggcc cgtggcgttt aacttaccga taagcgattt
     2341 gagattgacg ttcatggggc ctgcctcctt taggttgcct gcgaggggtg gccagataat
     2401 ttttgcaacc gcgttaaatg gcgtcctaag accgcctctg cggcatcgca agaaaactct
     2461 tttgtcttaa gccagctcga ccagccgagt cgcagcgctc ccggccctgg cttattcaat
     2521 cgacagcccg gcgcttctgc tgcttgtaaa accaattgga catcaaaatc aagcgttaaa
     2581 ccgacaaaaa aacgggtcag ttccacgagc gaactaaatc cattctcaga aggaagaaat
     2641 ccgcga
//
LOCUS       DASUVS010000231         6264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1185314, whole genome shotgun sequence.
ACCESSION   DASUVS010000231 DASUVS010000000
VERSION     DASUVS010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185314"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1480)
                     /locus_tag="VF182_12725"
     CDS             complement(158..1480)
                     /locus_tag="VF182_12725"
                     /inference="COORDINATES: protein motif:HMM:NF024877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase domain-containing
                     protein"
                     /protein_id="HEX6437993.1"
                     /translation="MAVTLQTPSEKSDHSTNSQASVPPPYLGRTVRIVGPVVNVDMNQ
                     SPHPKNQRGELGKPLFNWYHNTVSKDPLTRVSAPNHFADRRHFLSTIINQATKGKVNP
                     RKIEVPDAAAMSRHIKAVAHYMGADVVAIAQAHPSFLYAGSRYVQDGTAEDAYQETSP
                     TDLVKKFPYIIVATTAWDYNKLQAHRHLIGDAAYHVSQIKGNMILKALEGYIKELGYT
                     ALRGVCTPQAAGVASGVGELGRNGLVINKTYGARIHMPDPILTDLPLIADQPVDNAVE
                     DFCKICRKCANTCPTNSIPFGDKVVHNGIEKYKINWLTCYKLRPYVHDYWGSCLTCAA
                     VCPFTKPNVWWHSLATWSLRTCPIPARPALVHALKWIDDRFWGVARTSRVRWLGYDSG
                     IKPGDQACTVSGCNASHEGKGPAKEVAGNLGYYAPLKENTNRFVKRDA"
     gene            complement(1603..2001)
                     /locus_tag="VF182_12730"
     CDS             complement(1603..2001)
                     /locus_tag="VF182_12730"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX6437994.1"
                     /translation="MAIPGEKAIQMVRNAHTQATQLDIAVTAVVVDQSGRMIALGRMD
                     RARPITVEIALNKAYTAASFQQPTKELAGVAGQTWFQSLVVSSNGRIMPGAGALPIVE
                     GGSVVGAVAVAGGTEDQDQRCAEVALASYQ"
     gene            complement(2274..3809)
                     /gene="gndA"
                     /locus_tag="VF182_12735"
     CDS             complement(2274..3809)
                     /gene="gndA"
                     /locus_tag="VF182_12735"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008100995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEX6437995.1"
                     /translation="MATQKPADIGLIGLAVMGENLALNIADHGFRVAVFNRTTQVMKD
                     FLAANPDTPGGLIGCATLPELLAAIKKPRKIIILVKAGPAVDAVTQQLMDAGLEKDDL
                     VIDCGNSLWTDTIRREKDYASHCRFFGSGVSGGEIGARFGPSLMPGGDARSWQHLEPI
                     WKAIAAKVDPETGKAIETAKPGKPVEGGIPCTAYIGENGAGHYVKMIHNGIEYIDMQL
                     ISEAYFLMRKLLGAEPDQLSQIFARWNEGDLDSYLIEITADILKQRDPQKPDAFLVDH
                     ILDSAQQKGTGKWTSVNALDMGIPANAIAEAVFARNLSALKDERVEASKQLVGPEYSH
                     SGNREELIDSVRDALYCSKICAYAQGFQLMRAAESEYRWKFDFASIAAIWRGGCIIRA
                     RFLQKITDAYQREPRLINLLLDPYFNEQIKKTQSRWRSVVAIAAQAGIAVPAFMAALA
                     YYDGYRSAELPANLLQAQRDYFGAHTYERTDQPRGRLFHLDWPVPDRPEIKISGSAEN
                     RKQ"
     gene            4017..4886
                     /locus_tag="VF182_12740"
     CDS             4017..4886
                     /locus_tag="VF182_12740"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6437996.1"
                     /translation="MQTDRYDYAPIIRRKPLHWPNKARVALMIAPNIEFFHIDKIIPG
                     ASSSQLPDVTGYALRDYGARIGVFRMMEVLDKHGIRATVLLNSDVCAHHAAIIEEGNK
                     RGWEWLGHGVTNNIRMNQYPAEEERAVIRQIKETIAGATGKAPRGWLGPGGGDESPNT
                     LDHLAAEGFDYVCDWGCDDQPVPMRVKSGRMIGIPYQQGLNDIRVIFQGGHTPKDWLQ
                     MVCDQFDTLYAEGASQPRVMTIPLHPFVIGLAFRIKYLDKALEYICAHEGVWKATGAE
                     IADHFYANFYQKP"
     gene            4901..5896
                     /locus_tag="VF182_12745"
     CDS             4901..5896
                     /locus_tag="VF182_12745"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437997.1"
                     /translation="MNLKRSVRFVGILIALAALGLDNADAADRVIIGNVSRTVEQLPN
                     YAATDKGFFAQEGIQGDVVLIGSTDTLIQALIAGQVNVAIVDPTPAINAVERGASLKI
                     VGGTVPIAAYTLVGCPKYKSIKELKGTSIGVVSLVSGSTIFLREMLKKEGLEINKDYS
                     IIQGGPTTQRLASLKGCNTSATMVLGGDLPRARELGFTEIARLHDYIPNLQFHSLIVD
                     SKWAEANSQLVVRYLKAMVRAMQWSHANKEEAAKLVTQRTGIPLKYTRENVEEYLSQG
                     VISRDGSVNREGFQRLIDLMGERLFKQRPYPAPEKYLDMSYLRRAHQELGLTAGR"
     gene            6005..>6264
                     /locus_tag="VF182_12750"
     CDS             6005..>6264
                     /locus_tag="VF182_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEX6437998.1"
                     /translation="MLPYNRRLKTKARSLRTKPTNAELHLWYRLRRKQILGVQFYRQK
                     PIANYIVDFYAPAAKLVVELDGAQHLEPGQTKYDAQRTRDLE"
BASE COUNT         1350 a         1744 c         1767 g         1403 t
ORIGIN      
        1 ggaaaaacgt tgccgggggg cgcattgcgg atcaactcgg ttacagtatt ggcgagatcc
       61 gccggcggcg tgttgataaa aaaatcccag tacgggtcgt tcttgtgagg atcccttctc
      121 agccagggca gtttgaacgg acccaatcgc aacgtcacta ggcatcccgc ttgacgaacc
      181 ggttggtgtt ctcttttaga ggcgcgtagt aaccgaggtt gcccgccact tctttggccg
      241 gacccttgcc ctcgtgcgat gcgttgcagc cggagacggt gcacgcctga tctcccggct
      301 tgatcccgga atcgtagcca agccagcgca cgcgactggt acgggcgact ccccagaagc
      361 ggtcatcgat ccatttcagc gcgtgaacca acgcaggtcg cgccgggatc gggcacgtgc
      421 gcaaagacca tgtagcgagg ctgtgccacc agacattcgg tttggtaaac gggcatacgg
      481 cggcgcaggt cagacagctg ccccagtagt cgtggacata cggccgcaac ttgtagcagg
      541 tgagccaatt gatcttgtat ttctcgatgc cgttgtgaac gaccttatcg ccgaagggaa
      601 tgctgttggt gggacaagtg ttggcgcatt tgcggcaaat cttgcaaaaa tcttcgactg
      661 cgttatcgac cggctgatct gctatcagag gaaggtcggt caggatcgga tcgggcatgt
      721 ggatgcgcgc gccgtaagtc ttgttgatga ccagcccgtt tctcccgagt tccccgactc
      781 ccgaagcaac gccggcggcc tgcggcgtgc agacgccgcg caatgccgtg tagccaagct
      841 ccttgatgta gccttcgagc gctttcagaa tcatgttgcc tttgatttgc gagacgtgat
      901 acgcggcatc gccgatcagg tggcgatgcg cctgaagctt gttgtaatcc caggcagtgg
      961 tagcgacgat gatatacggg aattttttca caagatcggt gggcgaagtt tcctgatagg
     1021 catcctctgc ggtgccatcc tgaacatagc ggctgccggc gtaaaggaac gatggatgcg
     1081 cctgagcgat cgcgacaacg tccgcaccca tgtaatgggc taccgcttta atgtggcgcg
     1141 acatcgccgc cgcatccggc acctctattt ttctcggatt gacctttccc ttggtcgcct
     1201 gattgatgat ggtgctgaga aaatggcgcc gatcggcaaa gtggttcggc gccgaaaccc
     1261 gagtcagagg atccttggag acggtattgt gataccagtt gaaaaggggc ttgcccaatt
     1321 cgccgcgctg atttttcgga tgcggcgatt gattcatatc gacattcacc acgggtccga
     1381 cgatccggac ggtacggccg aggtagggag gcggaacact tgcctgactg tttgtactgt
     1441 ggtcggattt ttcagacggc gtttgtaaag ttacagccat gatgcctccg gtagaagacg
     1501 ttcaacagtt caatcgttca acagttcaag gtctgagttc tcttacttcc gttctgacgt
     1561 tgaacgtttg aagcattgaa ccttgaacgg tactttctcg ttttattgat acgacgctaa
     1621 cgccacttcg gcgcagcgct gatcctgatc ttcggtgccg ccggctaccg caactgcgcc
     1681 gacaaccgaa ccgccttcaa caatcggcag cgcgcccgcg cccggcatga tgcggccatt
     1741 gctcgacacg accaggctct gaaaccaagt ttgcccggcg actccagcca gctccttggt
     1801 cggctgctga aaactggccg cggtgtaggc tttgttcagg gcgatctcca ccgtaatcgg
     1861 acgggcccgg tccattctac ccagcgcaat catccggcca ctctgatcga ccaccaccgc
     1921 tgtcaccgcg atgtccaact gcgttgcctg cgtatgcgcg ttgcgcacca tttgaattgc
     1981 tttttctccg gggatggcca ttgtcgttcg taaacctcct ggcacgctgc tgaaaaagac
     2041 tcatatcctt cgttgcactc tatcgtctcg ctacaactac ttgctagtac acctgcgctc
     2101 gccgattttc gcgcgcctcg gctctgagtt ctttttgagc agcctgcaaa tgcgcgtttc
     2161 catcaacctg ctagggtcag aatgccttaa tgtaacagca acaggtatgt aacagtcaag
     2221 cgtttctctt tgggcatatc atatgtagga gtgcagcatg ctgcgcccct actttattgc
     2281 ttcctatttt ctgcggaacc cgagatcttg atctctggac gatccggtac cggccagtcc
     2341 aaatggaaca gtctgccgcg cggctgatcc gtgcgttcgt aggtgtgcgc gccaaagtaa
     2401 tctcgctgcg cctggagtaa atttgccggc agctctgccg accgatagcc atcgtaatag
     2461 gccaatgccg ccatgaacgc gggaacggca atgcccgctt gcgcggctat ggctaccacg
     2521 gaacgccaac gcgattgcgt ttttttaatt tgctcgttga agtagggatc gagtagcaaa
     2581 ttgatcagcc gcggctcgcg ctgatacgca tcggtgattt tttgcaggaa acgggcgcga
     2641 atgatgcagc caccgcgcca gatagcggcg atggacgcga aatcgaactt ccacctgtat
     2701 tcactctcgg ccgcgcgcat gagctgaaat ccttgcgcat aggcgcagat cttcgagcag
     2761 tagagcgcgt cgcgaaccga atcgatcagt tcctcacggt tgccgctatg actgtattcc
     2821 gggccgacta attgtttgga tgcctcgact cgctcgtctt tgagcgcgct cagattgcgt
     2881 gcgaagaccg cctcggcgat ggcattggcc gggattccca tatccagggc gttgacgctg
     2941 gtccatttac cggtcccttt ctgttgcgcg ctatcgagaa tatggtcgac gagaaatgca
     3001 tcgggtttct gcggatcgcg ctgctttaga atatccgcgg tgatttcgat caggtaagaa
     3061 tcgagatccc cttcattcca gcgcgcaaaa atttgcgaga gctgatccgg ttcggcgcca
     3121 agcagcttgc gcatcaggaa gtacgcctcg gagatcaact gcatgtcgat gtactcgatg
     3181 ccgttgtgga tcatcttgac gtagtgaccc gcgccgttct cgccgatgta ggcggtgcaa
     3241 gggatgccgc cttcgacagg tttgcccggc ttggcggttt cgatcgcttt gccggtctcg
     3301 ggatcgacct tggcggcgat ggctttccag atgggctcga gatgctgcca agaacgcgca
     3361 tcgccgcccg gcatcaagga tgggccgaag cgcgcgccga tttcaccgcc ggaaactccg
     3421 gagccgaaga agcgacaatg gctcgcataa tctttttcgc gccgtatggt gtcggtccac
     3481 agactgttgc cgcaatcgat gaccaagtcg tctttttcca gcccggcatc catgagctgc
     3541 tgcgtcacgg catcgacggc tggtcccgct ttaacgagaa tgatgatctt gcgcggcttc
     3601 ttgatcgccg ccagcagctc gggcaacgtc gcgcatccaa tcagaccgcc cggcgtgtca
     3661 gggttggccg caaggaagtc tttcatgacc tgggtcgtgc gattaaagac ggctactcta
     3721 aagccatgat ccgcaatgtt cagcgccagg ttttcgccca taaccgccaa gccgatcagg
     3781 ccaatatccg ccggtttctg agtagccatg gcttatctcc ttttcgctaa agaccgggag
     3841 gcgtatatgt gatgctcgtg aggagtgtaa cagccggcag aaaaaacaaa agcgcccatg
     3901 gtgaaactca ttttcggccg tacggctgca tcgccggaag cgtggccgta aaaaaatctt
     3961 gacctaaata gctcgcgtgt cataggtatc atccagccaa gctcaaggag ttctaaatgc
     4021 aaaccgaccg ctacgactat gcgccgatta ttcgccgtaa gcctcttcac tggcctaata
     4081 aagccagagt cgcgttgatg atcgcgccca atatcgagtt ctttcacatc gacaaaataa
     4141 ttcccggcgc ttccagctct cagttgccgg acgtcaccgg ttatgcgttg cgcgattacg
     4201 gtgcgcgcat cggtgtgttt cgcatgatgg aagtcctcga caagcacggg attcgcgcga
     4261 ccgtgctgtt gaactccgac gtgtgcgcgc atcatgcggc gattatcgag gaaggcaata
     4321 aacgcggctg ggagtggctc ggccacggtg ttaccaacaa tatccggatg aaccaatatc
     4381 cggccgaaga ggagcgagcg gtaattcggc aaatcaaaga aaccatcgcc ggcgcaaccg
     4441 gaaaagcgcc gcgcggctgg ttgggcccgg gaggcggcga cgagagcccc aacacgttgg
     4501 atcatctggc ggccgaagga tttgattatg tttgcgattg gggttgtgac gatcaaccgg
     4561 tgccgatgcg cgtaaaatcc ggacgcatga tcggcattcc gtaccagcag gggctcaacg
     4621 atattcgggt catctttcag ggcggccata cgcccaaaga ttggttgcaa atggtctgcg
     4681 atcagtttga cactttgtat gccgaaggcg cctcgcagcc cagagtcatg actattccgt
     4741 tgcatccgtt tgtcatcggc ctggcattcc gcatcaagta cctggataag gcgctggaat
     4801 acatctgcgc tcatgaaggg gtgtggaaag ccacaggagc cgagatcgcc gatcatttct
     4861 acgcaaattt ttatcagaag ccgtgagtcc ggagacactc atgaatttga aacggtcggt
     4921 tagattcgtt ggaattttaa ttgccctcgc cgctttaggc ctcgacaacg ccgatgctgc
     4981 cgaccgcgtg atcattggca acgtctcgcg gacggtcgaa cagttgccca actacgccgc
     5041 cacggacaaa ggcttctttg ctcaggaagg cattcagggt gatgtcgttt tgatcggctc
     5101 gacagatacc ttgatccagg cgctgattgc cgggcaagtc aatgtcgcca tcgtcgatcc
     5161 gacgccggcc atcaatgccg tggaacgggg agcgtcgttg aagatcgtcg gcggcacggt
     5221 gccgatcgcc gcctacacgt tggtcggctg tcccaaatac aaatccatca aagagctcaa
     5281 aggaaccagt attggcgtgg tgagcctggt gtcgggcagc acaatatttc tgcgcgagat
     5341 gctcaagaag gaagggttgg agatcaacaa ggattattcg attattcagg gcgggccaac
     5401 gacccagcgc ctggccagct taaaaggatg caatacctcg gcgacgatgg ttctcggcgg
     5461 cgatttgccg cgggcgcgcg agcttggctt caccgaaatc gcccgcctgc acgattatat
     5521 tcccaacctg cagttccata gcctgatcgt cgacagcaaa tgggccgaag ccaacagcca
     5581 gctcgtcgtc cgatatctca aagccatggt tcgagcgatg cagtggtcgc atgccaataa
     5641 ggaagaggcg gccaaactgg taacccagcg caccgggatt cctctcaagt acacccgcga
     5701 aaacgtcgag gaatatcttt cacagggagt catcagccgc gacggttcgg tcaatcgaga
     5761 agggtttcag cgcttgatcg atctgatggg tgagcgcctc ttcaagcagc gtccctatcc
     5821 cgcgccggag aagtatctcg acatgagtta tttgcgccgc gcccatcagg agctggggct
     5881 gacggccgga cgatgattca aaactgaaaa ggctcttcgc gtaatcacgc ggtgaaacac
     5941 ggtaagaaat tctgctcttg taaggttcac cccctttgaa aaagggggat ttgggggatt
     6001 tccgatgtta ccatacaatc gccgacttaa gaccaaagct cgctccttgc ggacaaagcc
     6061 gaccaatgcc gagctacacc tatggtaccg cctgcggcga aaacaaatcc tcggtgtgca
     6121 gttctatagg caaaaaccca tcgcaaacta catcgtcgat ttttatgcac cggcggccaa
     6181 actcgtcgtc gaactcgatg gcgctcagca tttggagccc ggccaaacaa aatatgacgc
     6241 acaacgcacc cgggatctgg agca
//
LOCUS       DASUVS010000232         6087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1403923, whole genome shotgun sequence.
ACCESSION   DASUVS010000232 DASUVS010000000
VERSION     DASUVS010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403923"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1012)
                     /locus_tag="VF182_12755"
     CDS             complement(35..1012)
                     /locus_tag="VF182_12755"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6437999.1"
                     /translation="MTKKIIFFALCSLLLAPCSGVDAQQSGKTFRIGFLDNGTASGSA
                     VLLDVFRQELSNLGWIEGKNLTIEYRFAEEKNDRLHEFAADLVRLKVDLVVVTGGPTA
                     LAAKRATSTIPVVMTNAADPVGVGLVASLARPGGNVTGFSSLGELSSKRLEILKDAVP
                     KLSRVGVLQPAGGSGRQLKELRPAAQALKLKLEEIETQPDAKGFESAFQIAKQKQVGA
                     IMTTTGRRFFAERKRIVELAVKYRLPTIYPQKEFVDAGGLMSYGADYDDLYRRAAGYV
                     DKILKGAKPADLPVQQATKFEFLINLKAAKHIDLTIPNRVLERANQVIK"
     gene            complement(1454..2563)
                     /locus_tag="VF182_12760"
     CDS             complement(1454..2563)
                     /locus_tag="VF182_12760"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438000.1"
                     /translation="MKSGFKILDSDMHLREPADLWDKYMEAEFRDQAPKILSSTARSS
                     AMVLLDGKILQGYQPTYRGGIFDATRIDSEIAEARARKFDSGSQLQAMDREGLDVAAL
                     YPSIGLGIMMREQINPKLASAVARAYNNWLYDFCQADPKRLKGVAMLPLHDPAEAVKE
                     AQRTVGKLGFVGVFARPEPLHDLPWHSRYFDSLWSALEELGVPLGFHSAAAAGELPQI
                     GDRFGDNLLLRHVVSHPMENMLAMADIIGGGVCDRHPKLKLAFLECYCGWVSFLLHRL
                     DNAMEKGRFPTAGKLKPSEYFQRQCWISTEHEKELPMIISLVGDNKIVFSTDYPHGDS
                     DFPLAVEEFLEMDGVSKESQKKILWDNCAQLYGLN"
     gene            complement(2748..3671)
                     /locus_tag="VF182_12765"
     CDS             complement(2748..3671)
                     /locus_tag="VF182_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX6438001.1"
                     /translation="MNESLIEMRAKPSDSTRVPLTIKDFKGKAEPDWCPGCGDFGVLS
                     ALKQALAELAIRPHEAMVISGIGCSSNLPGYISTYGMHTLHGRALSVATGAQMANHEL
                     KIIVTGGDGDGYGIGGNHFIHSMRRNVDLTYIVMNNQIYGLTTGQLSPTSVKGMATKS
                     TPFGSVENPLNPIPLAIAAGATYVARGYTGQVKHLVELIKGGIQHKGFSLIDAFSPCV
                     TFNHENSHEFFKQRTFKLEERKHDPTDFAAAMKYGYEWGDEIAIGLFWKRTDLPSLDQ
                     LETGLADGGPLARRPLGISPEAAQSLIRELM"
     gene            complement(3843..5633)
                     /locus_tag="VF182_12770"
     CDS             complement(3843..5633)
                     /locus_tag="VF182_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX6438002.1"
                     /translation="MAKFDLSVAIGGAAGQGIATPGDILARIFVRRGLHLNAYNAYQS
                     IVRGGHIFLTLRTSDEPVLSFGDKLDVMIPLNQDTMNRHLGLMKAGSAVLYNGDKIKA
                     DDVAPGVQLCPFSVKELAPNIKGDLVQNTIALAAVLQLIGVEFKILADILSLQFKRKG
                     DAVIAENVGVARSGYEYAAKHFRSLPFSLPETRKPLAFFEGNQALAMGGAAAGVRFYC
                     AYPMSPSSGVLHWMARHARQLGIMVRQVEDEIGVINMAIGAAHAGCRAMCATSGGGFA
                     LMTEGLGAAAMMEIPLVCINVQRAGPATGVPTKTEQGDLWQVMGAGQGDYPRIIVAPT
                     NIADCFKTVPELFNLADKFQCPGIILSDLLLSEGRSSIDPAELDFSVPIERGAVIGLN
                     GSLPPLNGGYKRYEFTETGVSPRALPGTPGFEHVVATDDHDEYGVLISDEYTNPHKRQ
                     AMHEKRMRKMEAILPSIDPPQLWGAKNAQVTLLGWGSTEGVIREAIQKLASEEGIYAN
                     ALQIKWIVPLHAAAITSALSNSKKVVIVENNYSGQFARYLRSETGIVADAHIRKYDGE
                     PFMPHHIVDGLKAILAGRTQQYVPVHEIMV"
     gene            complement(5657..>6087)
                     /locus_tag="VF182_12775"
     CDS             complement(5657..>6087)
                     /locus_tag="VF182_12775"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX6438003.1"
                     /translation="SEAHSVVRKRVPNTAGQSPCLNARRTGFFILRAERTFMATYIAL
                     VNFTDQGVRQIRQTTERAKALVNAGSNLGVKIKDIYWTMGAFDAVFTAEAQDDDAIMA
                     FAASMGALGNIRTRTMRAFSAAEMNKILETVPAGNFISAK"
BASE COUNT         1303 a         1754 c         1614 g         1416 t
ORIGIN      
        1 aattcttact attgcgcact gcctgttgcc ttcttcactt aatcacttga ttcgcccgct
       61 cgagcacgcg gttcggaatc gttagatcaa tgtgtttagc cgccttaaga ttgatgagga
      121 attcgaactt cgttgcctgc tgcaccggca aatcagccgg tttggcgcct ttcaaaatct
      181 tgtccacgta accagccgcg cgccgataga ggtcatcgta gtccgcccca taggacatga
      241 ggccgcccgc atcgacaaac tctttctgtg ggtaaatagt cggtaaccgg tatttgacgg
      301 caagctcgac gatccgcttt ctttccgcga aaaagcgccg accggtggtc gtcataatcg
      361 cgcccacctg cttctgcttt gcgatttgaa aggcgctctc gaaacctttg gcgtcgggtt
      421 gagtctcgat ctcctccaat tttagcttca gtgcctgagc cgcaggcctg agctctttca
      481 gttggcgccc tgatcctccc gccggctgca gaactccgac tcgggagagc ttgggaaccg
      541 cgtccttgag tatctctagc cttttgctac ttagctcacc taaactcgag aaccccgtaa
      601 cgttgcctcc cggccgcgcc agactggcaa ccaaacccac acccacgggg tccgcagcgt
      661 tcgtcatcac gacgggaatg gtgctagttg ctcgctttgc cgccaacgcc gttggtcccc
      721 ctgtgactac gactagatca accttaagac gaaccaagtc cgccgcaaac tcatgcaaac
      781 ggtcgttctt ctcctcggca aaccgatact caatggtgag attctttccc tcgatccatc
      841 caagattgct cagttcttgc cggaacacgt ccaagagaac cgcgctacca gaagcagtgc
      901 cattatccag gaaaccaatg cgaaaggttt ttccagactg ctgcgcgtcg actccagagc
      961 agggagcaag gagcagggag catagagcaa agaaaatgat ttttttagtc attgctcgcc
     1021 gtcctgcttt tccgatctct gacatctgac tgcagcctgt cctgagtgaa atcgaaggga
     1081 tctctgtcat ttgattactt cattcgcccg cttgagcgct tcgactggaa ttgtcagacc
     1141 aatctgtttt gccgctttaa ggcttatgat gaattcgaac ttcgttgcat gcgggcgact
     1201 tttgttgcct gctgcgcgtg agttggatta acagtcgcca gaaacaaggt agtaagtacg
     1261 aagaaaagaa attttttctc accggttttc ctgattgtct tcgttccttc cccctagacg
     1321 gggaaaggtt aggatagggg tgatgtggcc tcacatcaca agatcacccc tactctaact
     1381 ctcccccatc taggggagag aatcggaatc gaagatcgac ggacaactag ccatcgagac
     1441 agatttcatt ctcttaattg agcccataca actgcgcgca attgtcccac aaaatttttt
     1501 tctggctctc tttcgatacc ccgtccattt ccaaaaattc ttccacggcg agggggaagt
     1561 cggaatcacc gtgcggataa tcggtggaaa atacgatctt gttatcgccc accagactga
     1621 tgatcatcgg caactctttc tcgtgttcgg tcgaaatcca gcattgacgc tggaaatatt
     1681 cgctcggctt taacttgccg gcggtgggaa agcgtccttt ctccatggca ttgtcgaggc
     1741 ggtgcagcag aaacgaaacc caaccgcaat aacattcgag aaaggccagc ttgagcttgg
     1801 ggtgccggtc gcagacgccg ccaccgatga tgtccgccat cgccagcata ttttccatcg
     1861 gatgagagac cacatggcga agcagtaaat tgtcgccgaa acgatcgccg atctgcggca
     1921 gctcgcctgc tgctgcagcg gagtgaaaac ccagtggaac tccaagttcc tcgagcgccg
     1981 accataggct atcgaaataa cgggaatgcc acggcaaatc atgtaaaggc tcgggccggg
     2041 cgaaaacccc gacaaagccg agcttgccga cggtgcgctg cgcttctttg acggcttcgg
     2101 caggatcgtg aagcggcagc atcgccaccc ccttgagccg cttgggatcg gcctggcaga
     2161 aatcgtagag ccaattattg taagcgcgcg ccacggctga ggcgagcttc ggattgattt
     2221 gttcgcgcat cataattccc aatccgatgg acggatagag cgccgccacg tccagtcctt
     2281 cgcggtccat cgcctgaagc tggctcccac tgtcgaattt tcgtgcgcgc gcctcggcaa
     2341 tttcgctatc gatgcgcgtc gcatcgaaga tgccgccgcg gtaggttggc tggtaacctt
     2401 gcagaatttt accatcgagc aagaccatcg ccgagctgcg cgccgtcgaa cttagaatct
     2461 tcggcgcctg gtcgcgaaat tccgcttcca tgtatttgtc ccagagatcg gccggctcgc
     2521 gcaaatgcat atccgaatcc aggattttaa agccggattt caagagtgtt gcctccccgc
     2581 ttgtgatttt tgtgctagta acgaaaaaat attttcgttt ggagtatcgc tataacacgt
     2641 atggctgtca accgaggtag gagagagggg tactgagata gtggaatagt ggagtaaagg
     2701 agccttagac tcgagcactc cactactcca ttgctccaaa ttttctttta catcagctct
     2761 cgaataagcg actgcgcggc ctctggggaa attcccagcg ggcggcgcgc cagcgggccg
     2821 ccgtcggcga gcccggtctc caactgatca agggacggca aatcggtccg tttccaaaat
     2881 aggccgatgg cgatttcgtc accccattca tagccgtact tcatcgccgc ggcgaagtca
     2941 gtcggatcat gcttccgctc ctccaacttg aacgttcgtt gcttgaaaaa ctcgtgacta
     3001 ttttcgtgat tgaacgtcac gcaaggactg aacgcgtcga tgagtgagaa gcccttatgc
     3061 tgaataccgc ctttgatcag ctcgaccaag tgctttactt gcccggtgta gccccgcgcg
     3121 acataggtcg cgcccgcagc aatcgccagc ggaatcggat tgagcgggtt ttcaacgctg
     3181 ccgaaaggcg tgctcttcgt tgccatgcct ttgacgctgg tcggcgagag ctgtccggtc
     3241 gttaggccgt aaatctgatt gttcatcacg atgtaggtca ggtcgacgtt gcggcgcatg
     3301 ctgtgtatga aatggtttcc gccgatgccg tagccgtcac cgtcaccacc ggtcacgatg
     3361 atcttaagct cgtgattggc catttgcgcg ccggtcgcca ccgacagcgc ccggccatga
     3421 agtgtgtgca tgccataagt gctgatatag cccggcaggt tcgaagaaca gccgatgccg
     3481 ctaatcacca tcgcctcgtg tggccgaatg gccagttccg cgagcgcttg cttgagcgcg
     3541 ctcaggacac cgaagtcgcc gcagccggga caccaatcag gttccgcttt gcctttaaaa
     3601 tccttgatcg tcaatggcac gcgggtacta tccgacggct ttgctctcat ctcgattaag
     3661 gattcattca taagtgactc cttagtgctt tgtagaacaa ggtcaatgtc ggttgcgagc
     3721 tcagtcaaaa gcgtccagtg gcaaggcgcg cgacggatcg atcagcgcag gcgtacgtta
     3781 acagtacgtc ggagtcagac gatcaagcgc aacgccgcaa atgggccctt ttcagcggac
     3841 tgctaaacca tgatttcgtg caccgggaca tactgttgtg ttcggccggc taaaattgct
     3901 ttcaagccat cgacgatatg gtgcggcatg aacggctcgc catcatactt tcgaatgtgg
     3961 gcgtcggcga cgataccggt ctccgaccgt aagtaacggg cgaattggcc gctgtaatta
     4021 ttttcaacga tcacgacttt cttgctattc gagagcgccg atgtgatcgc ggcggcgtgc
     4081 agcggtacaa tccatttgat ctgcagagca ttcgcgtaga tgccttcttc gcttgccagc
     4141 ttctgtatcg cttcgcgaat gactccttcg gtggagcccc aacccaacag cgtgacttgg
     4201 gcgtttttcg ccccccacag ctgcggcggg tcgatcgagg gcagaatcgc ttccatctta
     4261 cgcattcgtt tctcatgcat ggcctggcgt ttgtgcgggt tggtgtattc gtcgctgatc
     4321 aacaccccat attcatcatg gtcatcggtc gccacgacat gctcgaaccc tggcgtcccc
     4381 ggcagcgcgc gcggagagac accggtttcg gtgaactcgt aacgcttgta tccgccgttg
     4441 agcgggggca atgagccgtt cagaccgatc accgcgccgc gttcgatggg aacgctgaaa
     4501 tccaattccg ccggatcaat gctcgagcgg ccttcggaaa gcaacaaatc cgacaggatg
     4561 atgccgggac actgaaactt gtccgccagg ttgaataatt ctggcacggt cttgaagcaa
     4621 tccgcgatat tcgtcggcgc gacgataata cgaggataat ctccctgtcc cgcacccatt
     4681 acctgccata gatcgccttg ctcggttttc gtcggcaccc cagtggctgg acccgcgcgc
     4741 tgtacgttga tgcagaccaa cggaatctcc atcattgccg ccgcaccgag cccttcggtc
     4801 atcaaggcaa atccccctcc cgaggtcgcg cacatcgctc ggcatccggc atgcgcggcg
     4861 ccgatggcca tgttgatgac accgatctcg tcttccactt gccgcaccat aatccccagc
     4921 tggcgcgcgt gccgcgccat ccagtggagc acgccggacg atgggctcat cggataggcg
     4981 caataaaagc gcacccccgc cgctgccccg cccatggcca gggcttgatt gccttcgaag
     5041 aacgcgagtg gttttctggt ctcaggcagc gaaaaaggta acgatctaaa atgcttcgca
     5101 gcgtattcat aaccggagcg cgccacgccg acgttttcgg caatcaccgc gtcgcctttt
     5161 cttttgaact gcaggctcag aatgtccgcg aggatcttga actccacgcc gatgagctga
     5221 aggaccgcgg caagcgcgat cgtgttttgc accaagtcgc ctttgatgtt cggcgccagc
     5281 tctttcaccg aaaatggaca gagctgaacg ccgggagcga cgtcgtccgc tttgatttta
     5341 tcgccattgt agagaacggc agagcccgct ttcatgagtc ccaggtgccg attcatcgtg
     5401 tcttggttga gcggaatcat aacgtcgagc ttatcgccga agctgagcac gggctcgtcg
     5461 ctggtccgaa gcgtgaggaa aatatgccca ccccgaacaa tcgactggta cgcattgtaa
     5521 gcattcaggt gcagcccgcg gcgaacgaag atccgcgcga ggatgtctcc cggagttgcg
     5581 attccttgac cggcggcgcc gccgattgcg accgaaaggt cgaatttcgc catgaagttc
     5641 cccttttggc tctaactcat ttcgctgaga taaaatttcc cgccggcacc gtttctagga
     5701 ttttattcat ctcggctgcg gaaaaggccc gcatggtccg ggtccggatg tttccgagcg
     5761 cgcccatgct tgcggcgaag gccatgatag catcatcgtc ttgggcctcc gcggtaaaga
     5821 ccgcgtcaaa agcgcccatg gtccagtaga tgtctttaat tttcacgccg agattcgacc
     5881 ccgcattaac cagggccttg gctctttcgg tggtctggcg aatttgacgg acaccttgat
     5941 cagtaaaatt gaccagcgcg atgtacgtcg ccataaaggt cctttccgcc cgcaaaataa
     6001 aaaagccagt gcgccttgcg ttcaagcaag gcgactggcc tgcggtgttt ggtaccctct
     6061 tgcggacaac actgtgggct tcacttc
//
LOCUS       DASUVS010000233         9656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_967100, whole genome shotgun sequence.
ACCESSION   DASUVS010000233 DASUVS010000000
VERSION     DASUVS010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9656
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967100"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VF182_12780"
     CDS             complement(<1..489)
                     /locus_tag="VF182_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HEX6438004.1"
                     /translation="MTDLRRQYYSRAMATKPRPRNFTAGLLDWIERWGNKLPHPFNLF
                     ILFSLVVLIFSFLAHALGLAVVHPVTGKQITAVNLLDRQGIQNIITKAVGNFTSFAPL
                     GTVLVAMIGVGVAERSGLFSAALKGFVRSVPSWLITAALVFAGVNASLTVDAGYVILV
                     PLG"
     gene            771..2879
                     /locus_tag="VF182_12785"
     CDS             771..2879
                     /locus_tag="VF182_12785"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX6438005.1"
                     /translation="MPKHRVTVDTGGTFSDFVFFNEGSGEISITKLPSTPREPFQAVL
                     NGVKELLDRGVAAGDVSFFCHGTTVGTNALLEEKGAKTGLLVTEGFRGIYEVMEQTRG
                     YGPATYDLFFEKPRLLAPPFYTEEIPERVDFRGNVLKPIDVEAARQAIRRLKLKGVQS
                     VAVCFLFSFLNPDHELKIKELFAEEFPAARLSLSYEVLPQIREFYRMSTTVINAYIQP
                     VMANYLGTLENRMRDMGITTPKLYIMQSNGGVSTFAGSAKKPVATVLSGPAGGVIASM
                     GTCERVGINNIITFDMGGTSCDVALIHQGNPVITTQGKINLRPIALPMLDIHTVSAGG
                     GTIARLDAVGGLQVGPDSAGADPGPVCYDRGGENITITDANMVIGVLDPDHFLGGRMK
                     LNKTKARHLLEERIAKPLAMNLMEAAAGVLDIINVKMEEAIKAVSSQRGYDIRDFTLV
                     AFGGGGPMHAGRMALDLGIPSVLIPLTPGVHSALGLLMSDVKHDYVRSKLVGLDDLDL
                     DEINPMFGQLIDQAKADLYGEGFRDSEIKMETYLDLRYAGQGYELTVPCPMPPLSKDG
                     LALMRARFDTVHEQNSGHKAETERVELVSLRLISLGIVPQAKLSPGKVSGRAVEEAMT
                     GTRKVFFGKQHGMLNTQIYSRDALEPGHKLSGPAIVEQLDTTTVIHPEQEAIVDNYRN
                     LIIKVETQYAAALQDIEKRS"
     gene            2876..4612
                     /locus_tag="VF182_12790"
     CDS             2876..4612
                     /locus_tag="VF182_12790"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX6438006.1"
                     /translation="MTVVDPVTLQVIQARLAGIVQEMQNSLFRTGYSTIIRESQDASC
                     AILNRQGEVVAQHVVLPLHMGAFPACAAGILKNYSAEEIHQGDAFITNHPYLGGSPHA
                     PDMAVLSPIFFRGEWVGFAANLAHKSDIGGPVPGSCWSQAREIYQEGLHVPPIKFVHQ
                     YQTSKDIEAVLGANSRTPELVVGDLRGQVGAARLGENRFQEMVERYGKETILSSFEQL
                     FALTEAKVRAEIASWPDGVGEGERFVDSDGIDLDTPVRLHVRMEKKGRDLLFDFTGCA
                     DQTKGPANIRPTIVRAACCYLLTCLVDPALAINYGLAKVIDIKVREGSVVNPRFPAPV
                     NTYNPTVHALVESLLEASSQITPHKKVGDGCSSRSIIIGGRSNKTGKGYVQYEIFGGG
                     SGGRSGKDGVSGTNVNQSNAKIAPIEIIESEFSTRLRKFQLIPDSGGPGTFRGGLGFV
                     REYEVLDNDARFSLRSTKHTVAPKGIDGGANGRTGHCTLNPGKADERAIPSRFSDHVL
                     HPGDVFSLETPGGGGLGNPLQRDPQRVVSDVRNGYVTRQKAREIYRVAVDEKDSDFIL
                     NESETRALRKSR"
     gene            4743..6479
                     /locus_tag="VF182_12795"
     CDS             4743..6479
                     /locus_tag="VF182_12795"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX6438007.1"
                     /translation="MLMVDPITLQVIQARLAGIVQEMQNSLFRTGYSTIIRESQDASC
                     AILNRQGEVVAQHVVLPLHMGAFPACAEAILKTYEPAQIQEGDAFITNHPYLGGSPHA
                     PDMGMFTPIFFRGECVGFAANMAHKSDIGGTVPGSGSGSAREIFQEGLHLPPVKFMSR
                     LRPIREIEAMIAANSRTPEVVIGDLRGQVGAARLGERRIAELMDRYGKQTILDCTDIL
                     SSNTEKRVRQVIATWPDGESEAESFVDHDGVDLSRPIRIHVKVKKTGDRIHFDFSGSS
                     DQTQGPANIRPPLVRAACVYCLVALVDRFLPINQGLARVVEAKFRPGSVVDPRFPAAV
                     NTYMPTALTVAEAVLTALATFVPEKRIAGGSGSAALVMGGRDAESQRAYVHYEIFSGG
                     TGARWSKDGVSATAFHLSNCKTAPVEIIESEFPTRIERFAMIPDSGGAGRLRGGLGFS
                     RDYRILADEVRFSMRTDKHTIEPFGSQNGLPGRKGSCIVNPGTHQEKRLSSRFGDQRL
                     SKGDLLRIERPGGGGLGNPMDRAAENVLEDVRQGYVSVGSAASDYKVAIRFSESEPCL
                     DLSRTQALRGKW"
     gene            6551..6826
                     /locus_tag="VF182_12800"
     CDS             6551..6826
                     /locus_tag="VF182_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEX6438008.1"
                     /translation="MATAKKKTRKPNAAFMKPVQPDAALSAVVGTKAIPRTEVTKKLW
                     AYIKKNGLQDKKKKTNINADDNLKVVFGGKKQVTMFEMTKLVSKHLK"
     gene            complement(7080..8507)
                     /gene="purF"
                     /locus_tag="VF182_12805"
     CDS             complement(7080..8507)
                     /gene="purF"
                     /locus_tag="VF182_12805"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX6438009.1"
                     /translation="MDKFHDECAVMGVYGHPEAANMVYLGLYALQHRGQESCGIVSSD
                     GKALISHRQMGLVADVFKEDVIKRLEGQSAIGHNRYSTQGQSHLKNAQPFVVEYSQGP
                     IAIAHNGNLVNGAILRDELERSGSIFQSTSDTEVIIHLIATSKEVSLMARIVEALSRA
                     RGAYSLLFITPDRMIAARDSYGFRPLVLGKFREGPNNGAYVVASETCALDLIEADYVR
                     EVEPGEIITFGPDGVESLKPFPPVPHAKCIFEYIYFARPDSTLYGHNVYSVRKSMGRQ
                     LARESAVSADLVTPVPDSGVPAAIGYAEESKIPLEFGLIRNHYVGRTFIEPQQTIRHF
                     GVKIKLNAQREVLNGKRVIVVDDSIVRGTTSRKIIRMLRDAGAKEVHMRISSPATTGP
                     CYYGIDTPTRTELIASSSSVEEIRRFIEADSLAYLSQDGMYDYFGGKQQGYCDACFSG
                     NYPVHFEDEGHTRQLHLFDALQHRS"
     gene            complement(8570..>9656)
                     /locus_tag="VF182_12810"
     CDS             complement(8570..>9656)
                     /locus_tag="VF182_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEX6438010.1"
                     /translation="RPAERPKSQDDLEQLDLSTIAEPRDLGATLKLMLAAPNIASKEW
                     VYRQYDCYVRSNTVVAPGADAAVVRIKGTDKGLALTVDGNSRYCFLDPYVGGALAVVE
                     AARNLACVGARPIGLTDCLNFGSPENPAVMWQFARVIQGMRDACIALKVPVVSGNVSF
                     YNETDGIPIYPTPTIGMVGLLPKVDRVITPWFKTAGDIVVLLGRTREELGGSEYLKYI
                     HNLVRGTPPWIDLRLEQAIQNCCIEAIEKGILRSAHDISDGGLAVALAECCIGGPDKP
                     LGVRAEMREMIRGDALLFSESQSRILVSMQEKDLSQLQEIATGHGVPLQVIGAVGGSR
                     FSIQPILQLPLDELQSIWSSALGNRLK"
BASE COUNT         2234 a         2701 c         2692 g         2029 t
ORIGIN      
        1 gccaagcggt actaagatga catatcccgc gtccaccgtg agactggcgt tgaccccggc
       61 aaacaccagc gcggcagtaa tcagccagga cggcacactt cttacgaatc ctttgagcgc
      121 cgcggaaaaa agcccgctgc gctccgccac accaacaccg atcatcgcca ccagcaccgt
      181 gcctaaggga gcaaagctgg taaaattgcc tacagccttg gtgatgatgt tctggatgcc
      241 ttggcgatcg agtaggttaa cggcagtaat ctgtttcccg gtcaccggat gaaccactgc
      301 caatcccaaa gcatgggcga ggaatgaaaa aataagaact acaagcgaaa agagtatgaa
      361 taaattgaac ggatgcggca gcttgttacc ccaacgctcg atccagtcca gcaaacctgc
      421 agtaaagttt ctcggacgcg gtttcgtcgc catcgccctt gaataatact gtcggcgtaa
      481 atcagtcaag cagacgcctc cgccggggcc gcgagcgagg ccctaccctg cacggaccag
      541 ctcagcaatt gtgacgcaag catcgagcat cctgcgcggc gatccatcgc acgcttcgcg
      601 ggctgtttca gatttgcagc cgcggggagt gggcgggcag caatcatcaa gccgcgacgg
      661 ttcaaggctc ttcctggggt aaatccaaca ctttcgtact gtcacaagag cgcttttcga
      721 ttgccaaagc tgattgaaga cccaggggat aaatgttaga cggaacacct atgccaaagc
      781 accgagtaac cgtcgatacc ggaggaacat tttccgattt tgtctttttc aatgaaggga
      841 gcggcgagat cagcatcacc aagctgccgt cgacgccacg ggaaccattt caagcggtgc
      901 taaacggcgt caaggaatta ctcgatcgcg gagtggcggc cggagatgtt tctttttttt
      961 gccatggcac cacagtcggc accaatgcgc tcctggaaga aaaaggcgcc aagaccggct
     1021 tgctcgtgac tgaaggcttt cgtggcatct acgaagtcat ggagcaaacc cgaggttatg
     1081 gccctgctac ctacgattta ttttttgaaa agccccggct cctggctccg ccgttttata
     1141 ccgaagaaat tcccgaacgg gtcgactttc gcggcaacgt attaaagccc atcgacgtcg
     1201 aagcggcgcg ccaagcgatc aggcgattaa agctaaaagg cgtgcagtcg gtggcggtgt
     1261 gcttcttgtt ttcgttttta aatccggacc acgagctcaa gatcaaagag ctctttgccg
     1321 aagaattccc cgcggcgcgg ctgtcgcttt cctatgaagt gctgccgcaa atccgcgagt
     1381 tttatcgaat gagcacgacc gtcatcaacg cgtatattca gccggtgatg gccaactatt
     1441 tgggcactct cgagaaccgg atgcgcgaca tgggcatcac cacgcccaag ctctacatca
     1501 tgcaatccaa cggcggcgtt tcgacgtttg cagggtcggc caaaaagccg gtcgcaacgg
     1561 ttctttcagg tccggccggc ggcgtcatcg cttcgatggg aacctgcgag cgcgttggca
     1621 tcaataatat catcaccttc gacatgggcg gcaccagctg cgacgtggcg ttgattcatc
     1681 agggcaaccc tgtcattacg acgcaaggga aaatcaattt gcggcccatc gcgctgccga
     1741 tgctcgacat tcatacagtc agcgccggcg gcggcaccat cgcccggctc gacgcggttg
     1801 gggggttaca ggttggaccg gatagcgcgg gggccgatcc gggtccggtc tgttacgatc
     1861 gcggcggtga aaacatcacc atcaccgatg ccaacatggt gatcggcgtc ttggatcctg
     1921 atcactttct cggcggccgc atgaagctca ataaaaccaa ggcccgacat ctgctcgagg
     1981 aaagaattgc caaacctttg gcaatgaact tgatggaagc ggcagccggc gtgctcgaca
     2041 tcatcaatgt caagatggaa gaggcgatca aggcggtatc gtcgcagcgc ggttatgaca
     2101 ttcgcgattt cactctagtc gcctttggcg gcggcgggcc catgcatgcc ggcaggatgg
     2161 ctctggattt aggcattcca tcggtcctga ttccgcttac gcccggcgtt cactcggctt
     2221 tgggcttgct catgtccgac gtcaagcatg actacgtgcg atccaaatta gtcggactcg
     2281 acgatcttga tctagacgag atcaatccca tgttcggcca gttgatcgat caagcaaaag
     2341 ccgatcttta cggcgagggt tttcgcgata gtgaaatcaa aatggaaact taccttgatt
     2401 tgcgctacgc cggtcagggt tatgagctga ccgttccctg ccccatgccg ccgctcagca
     2461 aggacggttt ggcgctgatg cgcgctcgct tcgatacggt gcacgagcaa aactccggtc
     2521 ataaagccga gaccgagcgg gtcgaactgg tgagccttcg gttgatttca cttgggatcg
     2581 ttccccaggc caagctctca cccggtaaag tctccggccg cgcggtcgaa gaagcaatga
     2641 cgggaacgcg aaaagtattt tttggcaagc agcacgggat gttgaacacg cagatctata
     2701 gccgggatgc gctcgaaccg ggtcacaagc tcagcggccc ggcaatcgtt gagcagttag
     2761 atacgacgac ggtgattcac ccggagcagg aagccattgt cgataattat cgaaatctca
     2821 tcatcaaggt agagacgcag tatgctgcgg ccctacagga cattgagaaa cgttcatgac
     2881 cgtcgttgat cccgtcacat tacaagtgat ccaagcccgt ttagccggca tcgtccaaga
     2941 gatgcaaaat tcgctttttc gcaccggcta ttcgacgatt atccgtgaat cacaagacgc
     3001 cagctgcgcg attttgaatc gccagggcga agtcgtcgcg cagcacgtgg tactgccgct
     3061 gcatatgggc gcatttcccg cctgcgccgc cggcatcttg aagaattaca gcgctgagga
     3121 gattcaccag ggtgatgcct tcatcaccaa ccacccgtat ctcggtggca gcccgcatgc
     3181 gcccgacatg gctgtgctgt cgccgatttt tttccgcggc gagtgggtcg gcttcgcggc
     3241 caacctcgcg cacaaaagcg acatcggcgg tcctgtgccc ggaagctgct ggagccaggc
     3301 ccgagagatc tatcaagaag gtcttcacgt gccgccgatc aagttcgttc accaatatca
     3361 aaccagtaaa gatatcgaag ccgttctcgg cgccaacagc cgcactccgg aattggtggt
     3421 aggagatctg cgcggccaag tcggcgccgc gcggcttggc gagaatcgct ttcaagagat
     3481 ggtggagcgc tacggcaaag aaacgattct cagctcgttc gagcaattgt ttgcattgac
     3541 ggaagccaag gtgcgcgctg aaatcgcctc ttggcccgac ggcgtcggcg agggcgagcg
     3601 gttcgtcgac agcgacggga tcgatctcga tacgcccgtg cgcctgcatg tgcgcatgga
     3661 aaagaaaggc cgggacctac ttttcgactt caccggctgc gccgaccaaa ccaaaggtcc
     3721 ggcgaacatt cggccaacca ttgtccgcgc agcgtgctgt tatctcctca cctgcctggt
     3781 agacccggcg ctggccatca attatggatt ggctaaggtc attgatatca aggttcgcga
     3841 aggcagcgtc gtcaacccgc gctttcccgc gccggtaaac acgtataatc caaccgttca
     3901 tgcattggtg gaatctctac tggaagcgtc gagccagatc acgcctcata aaaaggttgg
     3961 cgatggttgc agcagccggt cgattatcat cggcggccgc agcaacaaga ccggcaaagg
     4021 ctacgtgcag tatgaaatct tcggcggcgg ctcgggcggc cgcagcggca aagacggcgt
     4081 ctccggcacc aatgtcaatc aatccaacgc gaagatcgcg cccattgaaa tcattgaatc
     4141 ggaattctct acgcgcttaa gaaaattcca gctgatcccc gattcagggg ggccgggaac
     4201 atttcgcggc ggcttggggt ttgtccgaga atacgaagtg ttggacaatg acgcgcgttt
     4261 ttcactgcga tccaccaaac acacggtcgc gcccaaagga atcgacggcg gcgccaatgg
     4321 caggaccggt cactgcacgt tgaatcccgg caaggccgac gagcgggcaa ttccatcgcg
     4381 cttctccgac cacgtactcc atccgggcga tgtttttagc ctggaaacgc ccggcggcgg
     4441 cggtctggga aatccgttgc aaagagatcc tcaacgagtt gtgagcgatg tgcgcaacgg
     4501 ttatgtgacc cgacaaaagg cgcgagaaat ctaccgtgtt gcggttgatg aaaaagacag
     4561 cgatttcatc ctcaatgaat ccgaaacacg cgcgctgcgg aaatcgcggt aactgtgcca
     4621 atgcttcacg gacagctgga acgattggaa ctgttggaac ggctggaatg caaaagccgc
     4681 aaatcgcaaa ctttcgaatt gtgccagatg ttccatctct aatcgcactg ggcggctaac
     4741 caatgctcat ggtcgatccg atcactctcc aggtcatcca ggcgcggctt gccggcatcg
     4801 ttcaggagat gcaaaattcg ctcttccgga ccggctattc aacgattatc cgcgaatcgc
     4861 aagatgccag ttgtgcaatt ctaaatcgcc aaggcgaagt ggttgcgcag catgtcgtgc
     4921 tgccgctgca catgggcgcg ttcccggctt gcgccgaagc catattgaaa acctacgagc
     4981 ccgcccagat tcaagaaggc gatgctttca tcaccaatca tccctatctc ggtggcagcc
     5041 cgcacgcgcc ggatatgggc atgttcacgc cgattttttt ccggggagaa tgcgtcgggt
     5101 ttgccgccaa catggcgcat aagagtgata tcggtgggac cgtcccggga agcggttcgg
     5161 gcagtgcgcg tgaaatcttc caagagggcc tgcatttgcc accggtcaag tttatgtcgc
     5221 ggctgcgacc gatccgagaa atcgaggcga tgatcgccgc caacagccgc actccggaag
     5281 tggtcattgg cgatctgcgc ggccaagtcg gcgccgcgcg gcttggcgag cgccggatcg
     5341 ccgagctcat ggaccgttat ggcaaacaaa ccatactgga ttgcaccgac atcctgtctt
     5401 cgaacaccga aaaacgtgtt cgtcaagtga tcgcaacatg gcccgacggc gaatccgaag
     5461 ctgaaagctt tgtcgatcat gacggtgtcg acctgagccg tccgatccgc attcatgtga
     5521 aagtaaaaaa gaccggcgat aggattcact tcgatttcag cggcagcagc gatcagactc
     5581 agggtcccgc caacatacga ccgccgctgg tgcgcgccgc ctgcgtctat tgtctggtcg
     5641 cgctggtcga tcgttttttg ccaatcaatc agggtctcgc acgagtagtg gaggcaaagt
     5701 ttcgccccgg cagtgtggtg gatccccgtt ttccggcagc ggtaaacacc tacatgccga
     5761 cggcattgac agtcgccgaa gcggtcctta ccgcgctggc cacctttgtt cccgaaaaac
     5821 gaatagccgg tggatcgggg agcgccgcgc tagtgatggg cgggcgggat gccgagagcc
     5881 agcgggccta tgttcattat gagatcttca gcggcggcac cggcgcgcgc tggagcaaag
     5941 atggcgtctc ggccacggcc tttcacctga gcaactgcaa gaccgcgccg gttgaaatta
     6001 tcgagtccga gtttcccacc cgtatcgagc gattcgcaat gataccggat tcaggcggcg
     6061 cgggccggtt gcgcggcggt ttgggtttta gccgcgacta ccggattctc gctgacgaag
     6121 ttcgtttttc aatgcgcact gacaaacaca ctattgagcc ctttggcagc caaaacggtc
     6181 tgcccggccg caaaggatca tgcatcgtca atcccggcac gcatcaagaa aagcgtttgt
     6241 cgtcacgctt tggcgatcaa aggctcagca aaggggatct tctgcgcatc gagcgtcccg
     6301 gcggcggcgg ccttggaaat cccatggatc gcgcagccga aaacgtgctg gaagatgtgc
     6361 gccaaggcta cgtttcagtc gggagtgctg cgtccgacta caaggtggca atccgtttca
     6421 gcgagagcga gccgtgccta gatctatccc ggacgcaagc tttacgaggg aaatggtaaa
     6481 aaataagtag caaaaatagc cggcctctgg tataagcacg ctaatacacg cgccccttaa
     6541 aggaggaaaa atggcgacag caaagaaaaa gacccgcaaa ccgaatgcag cctttatgaa
     6601 gcccgtacaa ccggatgcag cattgagcgc ggttgtcggg acaaaagcta tccctcgcac
     6661 cgaggtaacc aaaaagcttt gggcctatat caaaaagaat gggcttcaag ataagaagaa
     6721 aaaaaccaat attaatgccg acgacaacct caaagttgtt ttcggtggca agaagcaagt
     6781 cacgatgttt gagatgacca agctggtcag caaacacctc aagtaaacag tcctccttaa
     6841 aatctcaata gcattgtagc ggcgatcaag atcggcgatc cctattgtcg atcttgatca
     6901 ctagcacgat gctgcggctt tcccctttga gcggctacat tgacgccgaa acgtattttt
     6961 gtgtttgacc gccgtattca ttcatttcgg ttgctttatt gaggcagtga ccttcagcaa
     7021 agtatcgcag ccggctcggt tgagtcagag attgtgaatg cgttcagcag aaggtgtcgt
     7081 tatgaccgat gctgtagcgc atcgaacagg tgaagctggc gggtgtgccc ttcgtcttcg
     7141 aaatgaaccg ggtagttgcc gctaaaacag gcgtcgcagt aaccctgctg ttttccgccg
     7201 aagtaatcgt acatgccgtc ctggctaaga tacgcgagac tatcggcttc aataaagcga
     7261 cggatctctt ccaccgaact ggacgatgca attagctcgg tgcgggttgg tgtatcgatc
     7321 ccgtagtaac aaggacctgt ggtggctggc gagctgatac gcatgtgaac ttctttggcg
     7381 ccggcatcgc gcagcatgcg aatgatcttc cggctcgtcg tgccacgcac gatcgaatca
     7441 tcaacgacaa tgacgcgctt gccgttgaga acttctcgct gggcgttgag tttgatcttg
     7501 accccaaaat gacgaatcgt ttgctgtggc tcaatgaacg tccggccgac gtaatggttg
     7561 cggatcaagc caaactcaag cggtatttta gattcctcgg catagccgat ggcggcggga
     7621 accccggagt ccggaaccgg tgttaccaaa tccgcgctca ctgcgctctc cctcgccagt
     7681 tggcgaccca tagatttccg aacactgtaa acgttgtgtc cgtacagcgt gctgtctggc
     7741 cgggcaaagt aaatatattc aaaaatacat ttcgcatggg gcaccggcgg aaaaggtttt
     7801 aaagattcca ccccatccgg accgaaggta ataatttccc cgggctcgac ctcgcgcaca
     7861 tagtcggctt cgattagatc cagggcgcag gtttccgagg cgacaacgta agctccgtta
     7921 ttgggtccct cgcgaaactt acccagcacc aatgggcgaa agccgtatga atcccgggcg
     7981 gcaatcatcc gatccggtgt aatgaacagc aacgaatagg cgccccgggc gcggctgagg
     8041 gcttcaacga tccgcgccat gagcgaaacc tctttggagg tggcgatcaa gtggatgatc
     8101 acttcggtgt cggacgtcga ttgaaatatc gagcctgagc gttcgagctc atcgcgcaag
     8161 atagcgccgt taaccaaatt gccgttatgc gcaatggcga tggggccttg ggaatactcg
     8221 acgacgaacg gctgcgcgtt tttaagatga ctctggcctt gagtggaata gcggttgtgg
     8281 cctatggcgc tttggccttc caggcgtttg atgacatctt ccttaaagac atcggcgact
     8341 aggcccattt gtctgtgcga gatgagggcc ttaccgtccg aagacacgat cccgcatgat
     8401 tcctgacccc ggtgctgcag cgcataaaga ccgagataga ccatgttggc ggcttcgggg
     8461 tggccgtaga cgcccatcac cgcgcattca tcgtgaaatt tgtccatgaa ttctcgaccg
     8521 cgaaattcag tccctttcaa acagttcggc ggccgcaatg gctgaccggt tacttcagtc
     8581 ggttgcccag ggcagatgac caaatcgact gcagctcatc gaggggcaat tgtaaaatcg
     8641 gttgaatcga aaaccgcgaa ccgccgactg cgccgatgac ctggagcggc accccgtgcc
     8701 cggtggcaat ttcttgcaat tggcttaaat ccttctcctg catcgatacc agaattcggg
     8761 attgtgattc gctaaataat agggcgtcgc cgcggatcat ttcgcgcatc tcggctctaa
     8821 ctcccaaggg cttatccgga ccgccgatac agcactccgc caatgctaca gccaagccgc
     8881 cgtccgatat gtcgtgagcc gaccggagga tgcctttctc gatagcttca atgcaacagt
     8941 tttgaatcgc ctgctccagc ctaagatcga tccaaggcgg tgtcccccta accagattgt
     9001 ggatgtactt gaggtattca ctaccgccga gctcttcacg ggtgcgtccc agcagcacca
     9061 caatgtcgcc agcagttttg aaccaggggg taataactcg gtcaaccttt ggcaggagac
     9121 caaccatccc gatcgttggc gttggataaa tgggaattcc gtctgtttca ttatagaaac
     9181 ttacattgcc gctcacaacc ggcactttga gggcgataca cgcatcgcgc ataccttgaa
     9241 tcacacgcgc aaactgccac atgacggccg gattctcggg actgccgaag ttcaagcaat
     9301 cggtcagccc gatgggccgt gcgccgacac aggccagatt gcgcgccgcc tcgaccaccg
     9361 ccagagcgcc gccgacgtag ggatcgagaa aacaataacg gctattgccg tcgactgtca
     9421 gcgcgagccc tttgtccgtc cctttgatgc gaactaccgc agcgtcagca ccaggcgcca
     9481 ccacggtgtt gctgcgaacg tagcaatcat attgacgata aacccattcc ttggaagcaa
     9541 tgttgggcgc ggccagcatc agctttaacg tcgctccgag atctctcggc tcggcgattg
     9601 tcgataaatc cagttgctcg agatcgtctt gactcttggg ccgctcggcg ggccgg
//
LOCUS       DASUVS010000234        11483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1188098, whole genome shotgun sequence.
ACCESSION   DASUVS010000234 DASUVS010000000
VERSION     DASUVS010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11483
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188098"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..276
                     /locus_tag="VF182_12815"
     CDS             58..276
                     /locus_tag="VF182_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438011.1"
                     /translation="MANLFYNDRLIIAYAAFQQSSKLWSAGAEITWKINGQRQSHTIE
                     ALPDRFKTAGEAERFVINFAKSWIDANP"
     gene            complement(325..1653)
                     /locus_tag="VF182_12820"
     CDS             complement(325..1653)
                     /locus_tag="VF182_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438012.1"
                     /translation="MPKKMFWLSAVTLSLTFVAVVSSSFAQTNPVFVQLGQAKGAVYK
                     PDSGPQFPVGILVMHRESNYMNNIACREFSRRGFVVLCMNSRFENNEALVDWELIPLD
                     VAQGVNHLKSVQKVNKVILYGNSGGGVTMSFYQAVAENGPSVCQGANKLVQCGNNLAG
                     IPPADGIILSDGHPGNPILRLRSVNPAVNNEKNPDRLNPRLDPFDAKNGFKPEGASHY
                     SDEFKARYFKGQAERMNRLIDDALELLQRVEAGKYIYTDDAPFNIPKFDGARLLSLDP
                     SIRHTTLFPEKLLKNDGTIAVQIVESLAPSRPELAEANQTFSEGAREGLTVRSFLSSN
                     AIRANNSMDESQIDLCSSNNSTPCMLQQVSVPLLAAANQASFQNLIQEVEINYLYANS
                     ADKDFIVVEGATTGVTPCTNCANPATSYANVTKNFFDYAAKWINDRFSSN"
     gene            complement(1718..3754)
                     /locus_tag="VF182_12825"
     CDS             complement(1718..3754)
                     /locus_tag="VF182_12825"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEX6438013.1"
                     /translation="MKQISLAPLVRSRSQVGAMLFVALGLATINPAPSADARIIRLEI
                     LSVQSPTFGGLSFGNVGTYEKIFARAHGEVDPSDRRNSLITDIDLAPRNSSGMVEYSA
                     DVHIIKPTDMSKGNQRIFYDVVNRGNKGHGSFNEVGGNNPTTAADAGTGLLMRRGYTL
                     VFSGWEDEGLVPPGNNRALARLPIARNPDGSSVVEHTITEVIFDNATGTDFELTYRAA
                     NLDHSQAKMLVHNHTNFIGGPLVERVEVPASVWSYVNDTTVRINRSDPFLAPYDQGAA
                     YEFVYPAKDPQILGLGLAATRDVVSFLRHESSAENPLRGAIEYVIAHGSSQSGRYLKG
                     FTYWGFNEDESGRKVFEGIQPKISGAHAIASNDRFGDTNATGRSYQRHLSAKQEFPFT
                     YEVRFDPISGLTDGIFARCQVTGTCPKVMHTDSANEAYLKPTALVTTDGLGNDIRLPD
                     DVRVFMVSGTQHGPSETPQTTATCQQLSNPNPHNPIVRALYMALDDWATKGIRPPASR
                     YPRVSDGTLVPSLPQSVQGFPVIPGVNYTGWYNPVDLLDKSTLPNMPIPGKSYTVLVP
                     KTDVDGNDIAGVRHPNIEVPIATYTGWALRRAPFAEGEDCALTGQYIPFAETKAERLA
                     EGDPRLSLEERYRNHGHYVSQVARAADRLVRQRLLLDEDSVDIITAAAESDVGK"
     gene            4132..5088
                     /locus_tag="VF182_12830"
     CDS             4132..5088
                     /locus_tag="VF182_12830"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX6438014.1"
                     /translation="MTLHQLRIFECVGTVSNITKASEILHISQPSVSQQLKLLEEEFD
                     AKFFARLSQGVELTYEGKQFLDAVKPILIQAEVVEKTFKRNPKANPMVLLKIGGSNNV
                     SSDVLPHLLMTFKQNHPLVQFTLETNDSPVIEKRVINSEVELGLITNPSYRSEIAYEP
                     YEEMQVVAFCLPIYPVAGKTLSLKELAQLPLVVRTGGRIEHALRSHRQSVNISVRCEA
                     SAAVKAAVGLGMGVGILYRNAVASRVANGTLKLIDVPELREMGVQSYIIFHKRKPISP
                     VAAEFREMLRKRDSVFWNDKKAQSSVTLPLRDKKDHSTKMTL"
     gene            5125..5727
                     /locus_tag="VF182_12835"
     CDS             5125..5727
                     /locus_tag="VF182_12835"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6438015.1"
                     /translation="MKNKISGLLVRLAIAVSILGLGWPEERQAVLHADDGDWDSPHVA
                     PFVTTPHEVVKRMLELAEIKKSDILYDLGSGDGRIVLAAAKNFKIRSVGFEIDPVLVK
                     ASRQLIKQAGLEELVEIREQDIRTIDFSPASVVTMYLYPAANLRLRPVLMRELKPGAR
                     VISHDFGMGSWEPDRVERLEDTAGLSRTIYRWRIAEPPGR"
     gene            6111..7457
                     /gene="thrC"
                     /locus_tag="VF182_12840"
     CDS             6111..7457
                     /gene="thrC"
                     /locus_tag="VF182_12840"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX6438016.1"
                     /translation="MSYNAWFQCINGCPGQFSLLEIMYRCPSCGDLLEVRHDMDALKT
                     RAAAAWIKLFDDRYRQNLYPYGSGVWAKKEWVVPFIDNENIVSTFEGNTNLFWAERYG
                     KQLHLEDLWVKQCGNSHTGSFKDLGMTVLVSVVKQMMADGMPIDAVACASTGDTSAAL
                     AAYGAAAGIPTVVFLPRNKVSPAQLVQPLANGALVLSLDTDFDGCMAAVQEVTKHKHI
                     YLANSMNSLRIEGQKTIAIEIVQQCDWEVPDWVVLPGGNLGNVSALGKGFLMMHELGL
                     ISKLPRICVAQAENANPFYRAFLTNFEGFEPITAKETLASAIQIGNPVSIRKAIATLQ
                     RFDGVVEQASESELSEETARADRTGMFNCPHTGVALAALRKLVERKVIRSHERVVVIS
                     TANGLKFADQKIAYHAGTLVGVQPGHQNRPVELSADPKQIAGAISHYVERMRLIDT"
     gene            7457..8719
                     /locus_tag="VF182_12845"
     CDS             7457..8719
                     /locus_tag="VF182_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6438017.1"
                     /translation="MADRGSKDSDKPKLPLQTQTGVRRTSKAAIDSQGIGTIAVHGGE
                     TRPKFGNALATPIVQTATYTFANTGELRDHFERRIEREEYGRYGNPTQRIAEQKLAEL
                     EAAEDCLLFASGMAAITTTLFAVLSHGSHVVVTDDSYRRTRQFLTQVLHRYGIEVSVA
                     PAGDYEAIEGAIRPTTRVLVSESPTNPYNYIVDLERFSEIGRRHRIKTIIDATFATPF
                     NQRPLDFGVDLVLHSATKYLGGHNDLLAGAVLGSAELIDGIRGLQAVTGAIADPFTAY
                     LLIRGLKTFALRLARQNATAQALAEFLQSHPRITAVYYAGLATHPQHDVACRQMRGFG
                     GVVSFEIAGDLDATSRVVDACRIPQIAPSLGGVESLIEQPALMSFYELTTEERLLVGI
                     KDNLIRYSVGIEDSDDLIADLTAALERP"
     gene            8781..8999
                     /locus_tag="VF182_12850"
     CDS             8781..8999
                     /locus_tag="VF182_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438018.1"
                     /translation="MAIFDSLLNVLRTLAVGDRVRITVRPETGVFPNPMDGEITAKDD
                     SGNLSLKSQQGPIQVKAGDVLSITKLGL"
     gene            9137..9850
                     /locus_tag="VF182_12855"
     CDS             9137..9850
                     /locus_tag="VF182_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6438019.1"
                     /translation="MNFRWTLFRIPIRSMLEARTLIALFLSVGAMLIFAEFVDRIVKG
                     ETRAFDERLLLALRDPNSPSHPIGPPWLQILFRDITALGGYAVVALITLAVIGYLLMD
                     DKAAAALWVLVSVGGGAVLSNLLKFAIERPRPDLVVRLVEVNTTSFPSGHATLAAVTY
                     LTLGALLSRVEARRRAKIYVLTVAVALTVLIGVSRIYLGVHWPTDVLAGWCIGAAWAM
                     LCWRIALALQRSGKIESDV"
     gene            complement(9910..10470)
                     /locus_tag="VF182_12860"
     CDS             complement(9910..10470)
                     /locus_tag="VF182_12860"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HEX6438020.1"
                     /translation="MMGSSGLLNTVLLLALWVAPGVAGQPEQGAFEVRIKDHREAIDD
                     FSRLLLTINGVAISPKPGLKFWQTSWQEFSPSPASVDLTKYIGKDSARILRADIDPGT
                     FDAVHLKVRSVDGLLKKNKRIAPVKNTIAPVKLSFDVAAGSETVLVLDLTVIDFSDHP
                     PRGYELSLKGYELYSNGKLIDKIPPG"
     gene            complement(10467..11099)
                     /locus_tag="VF182_12865"
     CDS             complement(10467..11099)
                     /locus_tag="VF182_12865"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX6438021.1"
                     /translation="MSPMTFKWRISLWIAAGSLGLELLSTPVFSHIPIPPKKGEIGRR
                     ESSSPAPNFVLTDESGKPFKFTAASGKLVLVTFVYTTCPDVCPLFTARLASIQRILEE
                     ENYDDYLLLTITTDPARDTPAKMKAYAQAFHADFRRWHFLSGSGEALAQVWKEFGVTV
                     RDLGNGQIQHTNLTTLIDPRGTRRVDYYGDKWQEKEILKDLARLRHSRPR"
     gene            complement(11068..>11483)
                     /locus_tag="VF182_12870"
     CDS             complement(11068..>11483)
                     /locus_tag="VF182_12870"
                     /inference="COORDINATES: protein motif:HMM:NF016337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX6438022.1"
                     /translation="TIQGEEEFNLVKEGTNWRVYLDWAAGIRVSYDAVVPPTDFIEAV
                     PVNRSTTIRSSELFTIAYRVKNRTAQTISTRITHRFEPKELSHHLEIVDCALLLPVEL
                     RPGEESEFSTTYIVDGDLPEGVKELNVTYDFQVAH"
BASE COUNT         2570 a         3027 c         3148 g         2738 t
ORIGIN      
        1 caagattgca gtgcaaggcg tggaggactt ctcctcggat ctctatagcc cacgatcatg
       61 gcaaatctat tttataacga tcggctcatc attgcctacg ccgcttttca gcaaagcagc
      121 aaactctgga gcgcgggcgc agagatcact tggaagatca atggccaacg gcaatcgcac
      181 acgattgaag ccttgcccga tcgattcaaa accgctggag aggcagaacg gtttgtgatc
      241 aactttgcta aaagctggat tgacgccaat ccctagctcg ccagcccgtc cgatcgtaaa
      301 aagctctgcc aatcttcctg cgaatcagtt agatgaaaac cggtcattga tccatttcgc
      361 tgcgtagtcg aagaaattct tcgtcacatt cgcataggat gtcgcaggat tcgcgcagtt
      421 tgtgcaagga gtgacgccgg tggttgcgcc ttccacaaca ataaagtcct tgtcggcact
      481 attagcatat aagtaattga tttcgacttc ctgaatgagg ttttgaaaag aagcctgatt
      541 ggcagcagcc agcaatggga ccgagacctg ctgaagcatg cagggagtgg agttattgct
      601 ggagcaaaga tcaatctgac tttcatccat ggaattattt gctcgaattg cgttgctgct
      661 taggaaagat ctcaccgtca gcccttcgcg ggcgccttcg ctgaatgtct gattggcttc
      721 tgcaagctcc ggccgcgatg gagcgagact ctcaacgatc tgcacggcga tggtgccgtc
      781 gttcttgagc agcttctcag gaaatagcgt ggtgtgacgg atgctcgggt cgagcgagag
      841 cagtcgcgct ccatcgaatt ttggaatgtt gaacggcgca tcatcggtgt agatgtattt
      901 acccgcctcg actctttgca ggagttcgag agcatcatcg atcagccggt tcatccgttc
      961 ggcttgacct ttgaaatacc gagctttgaa ctcgtccgaa tagtgcgagg ccccttcggg
     1021 cttaaagccg tttttggcat cgaatggatc gaggcgagga tttaagcggt cgggattttt
     1081 ctcgttgttc actgctggat tgacgcttct aagccggagg attggattgc cgggatgtcc
     1141 atccgagagg atgataccat cagcaggagg gatccctgcc agattgttgc cgcactggac
     1201 gagtttattg gctccttggc atacggaagg tccgttttcc gcaaccgcct gatagaagct
     1261 catggtgact ccaccgccgc tattgccgta caaaatcacc ttatttactt tttgaacgct
     1321 ctttaaatga ttcactccct gcgccacatc caggggtatc agttcccagt ccactaaggc
     1381 ctcattgttt tcgaatcgcg agttcatgca gagaaccaca aaaccccttc tcgagaattc
     1441 cctgcaggcg atgttgttca tgtagttcga ttcgcgatgc atcactaaaa tacccacagg
     1501 gaactgaggc cccgagtcgg gcttgtaaac cgctcctttg gcctggccga gttggacgaa
     1561 taccggatta gtttgggcaa acgaggatga aacgacggcc acgaacgtca ggctcaatgt
     1621 aacggctgat agccagaaca tcttttttgg cattttaatt cctctgctta ggtggcctca
     1681 atcaacagtg actcaacatc cgaatgctat acggcgctca cttgccgaca tcgctttccg
     1741 cggctgctgt gatgatgtcc accgaatctt cgtccagaag caggcgttgg cgaacgagac
     1801 gatctgctgc gcgggcgacc tgactcacat agtgaccgtg atttcgatag cgctcttcca
     1861 acgagagtct cgggtcgccc tccgcgagcc gctcagcttt cgtttccgcg aaaggtatgt
     1921 actggcctgt cagagcgcaa tcttctcctt ctgcgaaggg cgcacgccga agagcccagc
     1981 ccgtgtacgt tgctatcggt acttcgatgt tggggtgacg aacgcccgcg atatcgttgc
     2041 cgtcgacgtc tgtctttggc accaatacgg tatagctctt tccgggaatc ggcatattcg
     2101 gaagtgtgct cttatccaat agatcaaccg ggttgtacca tcccgtgtag ttcacgcctg
     2161 gaatcaccgg gaagccttga accgattgcg gcaaagacgg gactaaggtt ccgtcgctaa
     2221 ctctcggata gcgacttgcc ggcggcctta tcccttttgt cgcccaatcg tcgagtgcca
     2281 tgtagagcgc ccgaacgatg ggattgtgcg ggttgggatt actgagctgc tggcatgtgg
     2341 cggtggtttg cggcgtttcc gacggcccgt gttgagtgcc tgaaaccatg aatacgcgta
     2401 cgtcatcagg taatctaatg tcgttaccaa ggccgtctgt cgtcaccaga gcggtgggct
     2461 tgaggtaagc ctcgttggcg ctatccgtat gcatgacctt cggacaagtg ccggtaactt
     2521 ggcagcgcgc gaaaatgccg tccgtgaggc cgctgatggg atcgaaccgc acttcgtatg
     2581 taaagggaaa ttcctgctta gcgcttaagt gtctctgata actgcggccg gtggcgttgg
     2641 tatcgccaaa tcgatcatta ctggcgattg cgtgagcgcc ggaaatcttc ggttgaatac
     2701 cttcgaagac cttacggccg gattcatcct cgttgaagcc ccagtaagtg aagcccttca
     2761 agtagcggcc gctctgggac gatccatgag cgatgacgta ctcaatcgcg ccacgtaggg
     2821 gattctccgc cgaactctcg tgacgcagaa acgagaccac atcgcgtgtg gccgcaagcc
     2881 cgagcccgag tatctgcggg tctttggccg gatagacaaa ctcgtacgca gcgccctgat
     2941 cgtacggtgc tagaaatgga tctgagcgat tgatgcgaac tgttgtgtcg ttgacatagg
     3001 accaaacgct tgcggggacc tctacccgtt cgaccagggg acctccgata aaatttgtgt
     3061 ggttgtgtac gagcatcttt gcttgactat ggtcaaggtt agccgctcga taagtcaatt
     3121 cgaaatccgt tccggtggcg ttgtcgaaga tgacctcagt gatcgtatgc tccacaaccg
     3181 agctgccgtc ggggttgcgt gcgattggca gcctggcaag ggcacgatta tttcctggag
     3241 gcacgagccc ttcatcttcc caaccggaaa aaaccagcgt atagccgcgg cgcataagca
     3301 gaccggtgcc tgcatctgct gcagtcgtcg gattgttgcc gcctacctca ttgaaggagc
     3361 catggccttt gttgccgcgg tttaccacat cgtaaaaaat cctttgattt cccttagaca
     3421 tgtcggtggg tttgataatg tgcacatcgg ctgaatattc gaccatgccg ctggagttac
     3481 gcggcgccaa gtcgatgtcg gtaatcaaag agttccggcg gtcggacgga tcgacttcac
     3541 catgcgcgcg cgcgaagatt ttttcgtagg tgccgacgtt tccaaacgac aggcccccaa
     3601 acgtcggcga ttggacactc agaatctcca accgaatgat ccgcgcatca gccgatggcg
     3661 ccggattaat cgttgccaac cccaaagcga caaaaagcat cgcgccgacc tgcgaccgac
     3721 tgcgcaccag cggtgccagg ctaatctgtt tcatggccat cctccctgta actgaattaa
     3781 ggcggctcga cacaattcgg cataggagtg tcttcttttt cattcttctc ttcggattcg
     3841 cggtgtatct gtagcaggat agatttggca gtatgcggct acatttgaat gtcgcgtttg
     3901 atatttccag cgtgaacatt ctcgcaaaag taatcttcat tccgacgggg ctatcactcg
     3961 cataacggta tgggcctcta ccatttagcg aagcggattt tgcccatctt tgcatagaag
     4021 tgtccaatac tttcttcgaa tgccagccat tgactttcct tataacgctc cgtaaaaaac
     4081 aaataggacg atcctttcga tgttctctga gcgttttttg ggggagggat catgacactc
     4141 catcagctga gaattttcga atgcgttgga accgtttcga acatcaccaa agcttccgag
     4201 atcctgcaca tcagccaacc gtcggtttcc caacagttga aattactgga agaagaattc
     4261 gacgcgaagt tctttgctcg actcagtcaa ggtgtcgagc tgacgtacga gggtaaacag
     4321 tttcttgacg ccgtgaagcc tattttgatt caggccgaag tcgtcgagaa aacgttcaaa
     4381 agaaatccaa aagctaatcc aatggtgctt ctaaagatcg gtggaagtaa taacgtttcg
     4441 tccgatgttc ttccccatct gttgatgacc ttcaaacaga accatccgct ggttcaattt
     4501 accctcgaga caaacgatag cccggtgatc gaaaagcgag tgatcaattc ggaagtggaa
     4561 ctaggcttga ttaccaatcc atcataccgc tcagaaatcg cttacgagcc ttatgaggaa
     4621 atgcaggtgg tagcgttttg tctgccgata tatcctgtcg ccggaaaaac tctaagcttg
     4681 aaagaactgg cccaattacc cctcgtggtg agaaccggcg gaagaataga acacgcgctc
     4741 aggagtcaca ggcagtcggt aaacatttca gtccgctgcg aagcttcggc agccgttaag
     4801 gccgcagtcg ggttgggaat gggtgtggga attttgtatc gcaacgccgt tgccagccgg
     4861 gttgccaatg gcacgctaaa attgatcgat gtgcctgaat taagggagat gggggttcaa
     4921 tcttatatca tttttcacaa aaggaagccg atatcgcctg tcgcggcgga gttccgagaa
     4981 atgctccgga aacgcgactc ggttttttgg aatgataaga aagcacagag cagtgttacc
     5041 ctgccgctgc gcgataaaaa ggatcactcg acaaagatga ctttataaat gattgcattc
     5101 gggggccgac tagagatcct tcaaatgaaa aacaaaattt ctggtctgct tgtgcggctt
     5161 gccattgcag ttagcattct tgggcttggt tggccggaag agcggcaggc ggtgcttcat
     5221 gccgatgatg gtgattggga ctctccgcat gttgctccct tcgtcaccac cccgcacgag
     5281 gtggtgaagc gcatgctgga gctagccgag atcaaaaaat cggatattct ttatgatctt
     5341 ggatcgggcg atggaagaat cgttttagcc gcggcaaaaa attttaaaat tagatctgtc
     5401 ggctttgaga tcgatccggt gttggtgaaa gcttcgcggc aactcatcaa acaagccggt
     5461 ctggaagaat tggtcgaaat tcgcgagcag gatatccgaa ccatcgattt ttcacccgca
     5521 agcgtggtta ccatgtacct ctatcccgca gcgaacctgc gtctccgacc ggtcctgatg
     5581 cgcgaattaa agccgggcgc cagggtgata tcccacgatt tcggcatggg cagttgggag
     5641 ccagatcgag tggagcgtct ggaagacaca gccgggcttt cccgaaccat ctatcggtgg
     5701 cgcattgccg agccgccggg acgctgacct catgttgcgg tagagacggc tatgataagt
     5761 gatttctaca tcaaatcggt cggtcagttt ggcagagcga aaacacgctg gcggcgctat
     5821 tcgcgttctt cgaaagaagc tgtcgcttgt tcgtctgagt tcgtagtgca aaatttcacc
     5881 gagtcttcag acttgcgata cttcacgtcc ggatatttat tgcacagctt ttagagacct
     5941 tgagttgaca ctatcgaagg ctatcggcta ctctttagac tcccataaag ccgacggttt
     6001 gtctcactag tagctgctga ttctctgaac ctgagttggc tggcacaacc gttcttgggt
     6061 ctggaattga tctgtcccat tgccgctcaa gcggggaaag ctcttttatc atgagttaca
     6121 atgcgtggtt tcagtgcatc aacggatgtc cgggccagtt tagcctgctg gagataatgt
     6181 atcgctgtcc ctcgtgcggt gacttgctgg aagtgcgtca cgatatggac gccttgaaaa
     6241 cccgggcggc ggcagcatgg atcaagctgt ttgatgatcg ttatcgccag aatctgtatc
     6301 cctacggctc cggtgtttgg gcaaaaaaag aatgggtcgt gccatttatc gataacgaga
     6361 acattgtctc gaccttcgaa ggcaacacca acctgttttg ggcggagcga tacggaaagc
     6421 agctgcatct ggaagacctc tgggtcaagc aatgcggcaa ttcgcatacc ggctcattca
     6481 aggatctcgg catgaccgtt ctagtttcgg tggtaaagca gatgatggcc gacggaatgc
     6541 cgatcgacgc agttgcctgc gcctcaactg gcgatacttc cgcggctctg gcagcgtacg
     6601 gcgcggcagc cggcattccc acagtggttt tcctgccccg taacaaggtt tcccccgcgc
     6661 aactggttca accgctggcc aacggcgcat tggttctctc actagacacg gacttcgatg
     6721 gctgcatggc tgccgtccag gaggttacca agcataaaca tatctacctg gctaattcga
     6781 tgaacagttt gcgcatcgag ggccaaaaga ccatcgccat cgaaatcgtc cagcagtgcg
     6841 attgggaagt gcccgattgg gtagtcctgc cgggcggaaa tctcggcaat gtcagcgcgc
     6901 tcggcaaagg atttttaatg atgcacgagc tcggcctcat ctctaagctg ccgcgcattt
     6961 gtgtggcgca ggcggaaaat gcgaatccgt tttaccgcgc cttcttgacc aatttcgaag
     7021 gcttcgaacc catcacagct aaagagaccc tcgccagcgc gattcagatc ggcaatcccg
     7081 tcagtattcg caaggcgatt gcgaccctgc aacgtttcga cggagtagtc gaacaggctt
     7141 ccgaatcaga gctttccgaa gaaaccgcgc gcgcggaccg caccggaatg ttcaactgtc
     7201 ctcataccgg ggttgccctg gcggcgctgc gcaagctagt cgaacgcaaa gtgatacgct
     7261 cgcatgaacg ggtggtggtg atttcgaccg ctaacggcct taagttcgcc gaccaaaaga
     7321 ttgcctatca tgcgggcact ctcgtcggtg tgcagccagg acaccaaaat cgcccagtgg
     7381 agttgagtgc cgatcccaag caaatcgccg gtgcgatatc gcactatgtc gaacgcatgc
     7441 ggctgataga tacttaatgg ctgatcgcgg atctaaggat agcgacaaac ccaagttgcc
     7501 gctgcaaaca cagacgggag tccggcggac atccaaggct gccatagatt cccagggcat
     7561 cggcactatc gcggtgcatg gcggtgagac gcggccgaag ttcggcaatg cgctagcgac
     7621 accgatcgtg cagaccgcca cttacacctt cgccaatacc ggtgagcttc gcgaccattt
     7681 tgagcgacgc atcgagcgcg aggagtacgg ccgctacggc aatccaacgc aacgtatagc
     7741 cgagcagaag ctggccgaac ttgaggcggc cgaggattgc ttattgtttg cgagtggtat
     7801 ggccgccatt acaaccacgc tgtttgccgt gctctcgcac ggatcgcacg tggttgtcac
     7861 agacgactcc tatcgcagga ctcgtcagtt tctcacccag gtgctgcacc gttatggcat
     7921 cgaagtctcg gtcgccccag ccggcgatta cgaggctatt gaaggagcga ttcgtccgac
     7981 cacgcgcgtg ctcgtaagtg aatcgccgac taatccgtac aactacatcg tcgatctcga
     8041 gcgcttcagc gaaatcggcc gacggcatcg gatcaagacg attattgatg cgacgtttgc
     8101 gaccccgttt aaccaacggc ccctggattt cggcgttgat ctggttctgc attccgcgac
     8161 caaatacctg ggcgggcata acgatctcct cgccggcgcg gtgcttggat ccgcggagct
     8221 cattgacggc attcgcggct tgcaggcggt aaccggcgcc atcgcggatc cattcaccgc
     8281 ttatctcctc attcgaggtc taaaaacatt tgcactgcgg ctcgcgcgac agaatgcgac
     8341 cgcgcaagcc ctcgccgaat ttctccaatc gcacccgcgg attactgccg tgtattatgc
     8401 cggcctagcg acccacccgc agcacgacgt cgcgtgccgc cagatgcgcg gtttcggcgg
     8461 tgtggtctcg ttcgagattg cgggcgacct cgatgccaca tcgcgcgtcg tcgatgcctg
     8521 ccgtataccg cagatcgcgc cctcattagg cggggtggag agtctcatcg agcagcctgc
     8581 gctgatgagc ttttacgagc ttacgaccga agagcgcctt ctggtcggta tcaaggacaa
     8641 tctcatccgc tattcggtcg ggatcgagga ttccgatgac ctgattgccg atttaacagc
     8701 cgccctcgaa cgtccatgaa gtgcatcgct aatctcaatc gtaagatgat agagttctct
     8761 ttgctgctca gggagggatg atggcgatat tcgactcttt gctaaacgtg ctgcggacgc
     8821 ttgcagttgg tgatcgggta aggataacgg tccggccgga aaccggagtt tttccaaatc
     8881 caatggacgg agaaatcacc gccaaggatg acagcggcaa tctctctttg aagtcgcagc
     8941 agggaccgat tcaggtgaaa gccggcgatg ttttatcgat tactaaactg ggactgtaat
     9001 cgctgatatg gaatcggcct gttcaccgta gcattacgca gcgctgcttg ccttattgac
     9061 ccgcctggtc ccacgacgat actacgctcg gatatcgtag aaagggtttc ggaggtgata
     9121 gactgaccgt gagtctatga actttcgttg gaccctcttt cggataccga tccgctcgat
     9181 gctggaggcg cgaaccttga tcgcgctatt cctaagtgtc ggcgcaatgc tcatttttgc
     9241 tgaattcgtc gatcgcatcg ttaaaggcga gacccgcgct tttgatgaaa ggctattgct
     9301 ggcgttgcgc gatccaaaca gcccttcgca ccctatcggc ccgccatggc tgcagatcct
     9361 atttcgcgac atcaccgcgc tcggcggcta cgcggtggtg gcgctgataa cgctagccgt
     9421 gatcggttat ttgctcatgg atgataaagc cgccgccgct ctgtgggtgc tggtctcggt
     9481 cggcggcggt gcggtgctta gcaatttgct caagttcgcg atagagcggc ctcgtccgga
     9541 tctcgttgtt cgcctggtcg aggtgaatac cacaagcttc ccaagcggcc atgccacact
     9601 tgctgccgtg acttatctga cgctgggagc gctgctatcc cgagtggagg cgcggcggcg
     9661 tgccaaaatt tatgttttga cagtggcggt ggccctgacc gtgttgattg gtgtgagccg
     9721 catttacctc ggcgtccact ggccaacaga tgtactggcc ggctggtgca ttggcgccgc
     9781 ttgggcgatg ttatgctggc gcattgcgct tgcgctgcag cgaagtggga aaattgaaag
     9841 cgatgtctga ctgaaaatac atgccaagct gaggagagtc gaacttgcgg cgagaaaagg
     9901 ttagcaatcc taaccaggcg ggatcttgtc aatcagtttg ccattactat agagctcgta
     9961 gcctttgagg ctcaattcgt atccacgcgg cgggtggtct gaaaagtcga tgacggttaa
    10021 gtcgagaacg aggaccgttt cggaccccgc tgcaacatcg aacgacagtt tgaccggcgc
    10081 gatagtgttt ttcaccggcg cgatacgttt atttttcttg aggagaccat cgaccgatct
    10141 gactttcaga tggactgcat cgaatgtccc gggatcgata tcggcccgta atatcctagc
    10201 gctgtccttt ccgatatatt tcgtcaggtc gacagaggct ggagaaggac taaactcctg
    10261 ccagctggtc tgccaaaatt ttagccccgg cttgggactg atggcgaccc cgttaatggt
    10321 gagaagcaac cgagaaaaat cgtcgatcgc ctcgcggtga tccttgatgc gaacttcgaa
    10381 tgcgccttgc tccggctgac cagcgacacc gggagcgacc caaagcgcca gcagcagcac
    10441 ggtgttaagc aaacccgacg atcccatcat cgcggtctcg aatgccgcag tcgggcaaga
    10501 tcctttagga tttctttttc ctgccatttg tcgccgtaat aatcgacacg cctagtccct
    10561 cgcggatcga tgagtgtggt caaattggtg tgctgaatct ggccgttgcc cagatctcgg
    10621 accgtcacgc cgaattcttt ccacacttgg gccagcgcct cgccagagcc gctgagaaaa
    10681 tgccagcgtc taaaatccgc gtggaaagcc tgagcgtaag ctttcatctt tgccggagtg
    10741 tctcgcgccg gatcggtggt gatggttaga aggaggtaat cgtcataatt ttcctcctcc
    10801 agtattcgct gtatggatgc caatcttgcg gtgaacagag gacagacatc ggggcaagta
    10861 gtgtaaacaa acgtgaccag taccaacttg ccgctggcgg cggtaaactt aaagggcttg
    10921 ccggattcat cggttaagac aaagttaggc gcgggactgc ttgattccct gcggcctatc
    10981 tcgccctttt tcggtggaat aggaatgtgt gaaaagaccg gggtcgacag caactccagg
    11041 cccaaagatc ccgcggcaat ccacaggcta atgcgccact tgaaagtcat aggtgacatt
    11101 taactctttg actccttcgg gaagatcgcc gtcgacaata taagtggtcg aaaattccga
    11161 ctcctctccg gggcggagct cgacggggag aagcaaggcg caatcgacga tctccaggtg
    11221 atggctgagt tctttgggct caaaccgatg agtgatgcga gtcgagatcg tctgtgcggt
    11281 tcgattcttg acccggtatg caatggtgaa aagttcgctc gagcggattg tcgtggatct
    11341 gttaacaggg actgcttcga tgaaatctgt cggcggcact accgcgtcat aactcaccct
    11401 gatcccggcg gcccaatcga ggtaaacccg ccagtttgta ccttccttaa ccaagttaaa
    11461 ttcttcttcc ccctggattg tcc
//
LOCUS       DASUVS010000235         3590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_94708, whole genome shotgun sequence.
ACCESSION   DASUVS010000235 DASUVS010000000
VERSION     DASUVS010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94708"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1240
                     /locus_tag="VF182_12875"
     CDS             230..1240
                     /locus_tag="VF182_12875"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6438023.1"
                     /translation="MSAIFTAGVQLGQRNTSSRVDRTWWKNNAGDYTPFAMEQFDIAI
                     IGGGMAGLSAALYSGWLGRKALLAERQLFGGQVVNADQIENFPGFPDGILGADLVSQV
                     RMQALKLGAKMQYLETTAIKIDGGGFQLATTEEPIQASSVIVATGGKHRVLGIRGEIE
                     FEGRGVSRCATCDGAFFQNQPVAVVGGGDTALDEALYLSNIASRVTIIHRRATPIASE
                     TLVRRAREIPKIEFVANTTVVEILGTDSVEAVRLNDGTLVTVSAVFTAIGFEPETSLL
                     KDLVQLDPIGHAPVDIHMQTTVPGLFAAGSARQGNAGQLASAAGDGVTAAVAAHRYLA
                     NS"
     gene            1528..1815
                     /locus_tag="VF182_12880"
     CDS             1528..1815
                     /locus_tag="VF182_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438024.1"
                     /translation="MATLKMLDPRGAVNPGDRPVMRGLDSLEGKVIGIIDNGQSNSTA
                     MFQELAKLIHEKYQPAEVLFKTKPSHMQGAPRPILDEFVSRCDAVITGLGA"
     gene            1855..2061
                     /locus_tag="VF182_12885"
     CDS             1855..2061
                     /locus_tag="VF182_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438025.1"
                     /translation="MQKNGKPTVTLCQSIFMELAKTEAKFFGLTALPLVEIPHHPSGG
                     ARAGEHRADAEGAVNLVRQRLTLG"
     gene            2381..3448
                     /locus_tag="VF182_12890"
     CDS             2381..3448
                     /locus_tag="VF182_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438026.1"
                     /translation="MTTLTSPIIEVEDSWEAANDWFCREKLSDGLPIVPPTPERVTRM
                     LEGTDRDPQEVIGLIPPKWAPASIEKLAINAVMAGCLPEYLPVIIAAVEAICEPRFNL
                     YGVQATTGYVGPALLLNGPIRTALNVNCDSGVFGPGFRANATIGRAIRLILITVGGAY
                     PGDTDRSTFGWPGKYTMCFGENQEKSPWEPYHVEMGFTPNDSTVTVFGINGFLPIHTA
                     GNRGEQALSSLAEVLKMHHGVSHLDVGSFGGGTPLIGLGIEDAEIMARDSITKKQVKD
                     YLWEHASLKFTEVPARRKGHKSDDELLRESPNVTPDGLVHLSTKPEDIMVVVLGGKHR
                     HSVFLPMWTGRNTLSVIKPIR"
BASE COUNT          843 a          947 c         1024 g          776 t
ORIGIN      
        1 ctgccggtaa aatattccgc cggtcctttg ccggaaggct gtgagccact tcgtcgcatg
       61 tccattgcca atcccctttt tgtctgatcc aagactcact catcttgctg ttaccgatca
      121 aatctatcag tttcgctggc atgagattag tagggtcgcg ctagtttatg aactcgagtg
      181 cgttcataaa ggcctttcta acaagaaaat gggttctgtc gagcgcagat tgagcgcaat
      241 atttacggcg ggagtacagc tcggtcagcg caatacttcc tcgagagtcg atcgaacctg
      301 gtggaaaaac aacgccggag attatacacc gtttgctatg gaacaatttg acatcgcgat
      361 catcggcggc ggcatggccg ggttgagcgc tgctctgtac agtggatggc ttggacggaa
      421 ggcattgctt gctgaacggc agctgtttgg cggacaggtt gttaacgccg atcagatcga
      481 aaattttccc ggttttccag atggtatttt gggcgctgac ctggtgtcgc aagtgcgcat
      541 gcaggcgcta aagctcggcg caaaaatgca gtacttggaa acgaccgcga tcaagattga
      601 cggtggcggt tttcagttag ccaccaccga ggaaccgatc caagcaagca gcgtgatcgt
      661 ggcgaccgga ggcaagcacc gcgttctggg aattagaggg gagatcgaat tcgaaggccg
      721 aggagtttct cgttgtgcta cgtgcgacgg cgcatttttt caaaaccaac cggttgcggt
      781 ggtcggcggc ggtgataccg ctctcgacga agcgttgtat ctttccaaca tcgcctcacg
      841 ggtcacaatc attcatcgac gggcgacgcc catcgccagc gagactctag tgcgccgggc
      901 gcgagaaatc ccgaagatcg agtttgtcgc aaatacgacg gttgtagaaa tccttggcac
      961 ggattcggtc gaagcggttc ggttgaacga cggtactctc gtgacagtta gcgccgtatt
     1021 taccgctatc ggctttgaac ctgagacgag cctgttaaag gatctcgtgc agttagaccc
     1081 gatcgggcac gcgccggtgg acatccatat gcagaccacc gtgcctggac tctttgccgc
     1141 cggatcggcg cgtcaaggaa acgccggaca actcgcaagc gctgccggcg atggcgtcac
     1201 ggccgcagtg gcagcgcacc gctaccttgc aaatagttga atgagccgga ctattcatta
     1261 gtccggcact tatttgttcc attcacatct caaatagatc ccgcaacgta cgtttgaatt
     1321 aattgcgcct ttccaagtca atgtgaactc cgggatgatg tgaggacgta aaactgttcg
     1381 caactgttaa tctcaaggcg ccactggtct gaactgacag ttgcagcgaa tgaatgactt
     1441 gcatataaga aaccaacaac cgaagaattc ttcacgaatt ttgcgatcac agagaacact
     1501 gatcgcgttg taggaggata ctgttcgatg gcaacgctca agatgctcga cccgagaggc
     1561 gccgtaaatc ccggcgatcg acctgtgatg cgggggttgg attcgttgga aggaaaggta
     1621 atcggcatta tcgacaatgg ccaatccaat tccaccgcga tgtttcaaga gctggcgaaa
     1681 ttgattcacg aaaaatatca accagcggaa gtgcttttca aaaccaagcc ctcgcatatg
     1741 cagggcgcgc cgagaccgat cctggatgaa tttgtcagcc ggtgtgatgc ggtgattact
     1801 ggtctgggag cttgaggatc gtgcacgtcg tggagtgtcc acgacgccat cgagttgcaa
     1861 aaaaacggca agccgacagt gactctgtgt caatcgatct tcatggagct ggccaagacc
     1921 gaggcgaaat tctttggcct cacggcactt ccactggtgg aaatccccca tcatccgagc
     1981 ggcggtgcaa gagccggcga gcatcgcgcc gacgccgagg gagcggtgaa tttggtgcgc
     2041 cagcggctga cgttgggatg aaattgtgca cagctcacga ccgcgcacga gccggaaagg
     2101 tttagtggcg tctgaagcag atttcctttt cctccccgtc agaacatggg acagttagga
     2161 tgagggttga ggcacactgt tgcaacagcg agagaactac gcgccaatgc gacattgaaa
     2221 ccacgaagtt gaccgacatc gaaactgtgc agaatgttat atgcagtact ctgaacgcgg
     2281 cccccctcct gaatcctacc tgaggtgggg aggacgtatg tcggaacaat ctcaattgca
     2341 tacctaagag cttgatgacg attaatcgga gtaatcataa atgacaactc tcacctcacc
     2401 gatcatcgaa gtcgaagaca gttgggaagc cgccaacgat tggttctgtc gcgaaaagct
     2461 gagcgatggt ttgccgatcg tgccgccgac cccggaacgg gtaactcgga tgctcgaagg
     2521 caccgaccgt gatccgcagg aagtgatcgg cctcatcccg cccaagtggg cgccggcaag
     2581 catagagaag ctcgccatca acgcggtgat ggcaggatgt ttgccggaat atttgccggt
     2641 gattatcgcc gctgtcgaag cgatctgcga gccccggttc aacctctacg gcgtgcaggc
     2701 aaccaccggc tacgtcggtc cggcgctgct tctcaatggt ccgatcagga ccgcgctcaa
     2761 cgtcaattgc gactccggcg tgttcgggcc tggttttcga gccaacgcca ccatcggccg
     2821 cgcgattcgc ttgatactca tcacggttgg cggagcttat cccggcgaca ccgaccgatc
     2881 gaccttcggt tggccgggca agtacaccat gtgcttcggt gaaaatcagg agaaaagtcc
     2941 ctgggagccg tatcatgtgg aaatggggtt cacgcctaac gacagcaccg taaccgtttt
     3001 cggcatcaat gggttcctgc cgattcacac tgccggcaat cgcggtgagc aggcgctctc
     3061 ttcattggcc gaagtcttga agatgcacca tggcgtgagc catctcgatg tcggcagctt
     3121 cggcggcgga acgccgctca tcgggctcgg catcgaagac gcggagatta tggcccggga
     3181 cagcataacc aaaaagcagg tcaaggatta tctctgggag catgcgtcgt tgaaatttac
     3241 tgaagttccc gcgcggcgca agggacataa aagtgatgat gaactgctgc gagaatcgcc
     3301 caacgtcacc cccgacgggc ttgttcattt gtcgaccaag ccagaggaca tcatggtcgt
     3361 ggtgctcggc ggcaagcacc gccattcggt gtttttgccg atgtggaccg gccggaacac
     3421 tttatcggtg attaaaccga ttcgatgata acgggcgctg gagcgacgga gcggctcgcc
     3481 gcttacattc aaaagtcgcc gatttcctct atatagccaa tgcgacggtg gacacgctat
     3541 aaccccgaag tatatcgacc ccgcttatcg gcatcaggca ctggcgaaac
//
LOCUS       DASUVS010000236         7514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1188198, whole genome shotgun sequence.
ACCESSION   DASUVS010000236 DASUVS010000000
VERSION     DASUVS010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7514
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188198"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VF182_12895"
     CDS             <1..1010
                     /locus_tag="VF182_12895"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438027.1"
                     /translation="PFPRIQNQKFVQVAGLVLAFLTCAAPAEAQQRSTIPKIGYVSAT
                     GDSSNQGVYVEALRRGLKELGYVEGKNITVEYRGARGETGRIPRLVRELVQLKVDVLV
                     APVLPAIRAAKEATRTIPLIMVISEDPVAAGLVDSLARPGGNITGLTRLQRELSGKRL
                     ELLKQTIPRLARIAVLRDAESPSAVIAFKEYENAAHVLKLQLQSVGVLGSKPDLDGAF
                     QNAAAQRVNALITVTNANILRDQERVAELALRHRLPSMFEGSTWVDAGGLISYSTNDL
                     EIYRRAATYVDKILKGAKPSELPVEQPNKFELVINLRTAKQIGLTIPSNVLARADRVV
                     R"
     gene            1194..2171
                     /locus_tag="VF182_12900"
     CDS             1194..2171
                     /locus_tag="VF182_12900"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438028.1"
                     /translation="MSIGAQAIRLILVLLSFPALALAQPVQPVVRVGALHLGTTKTAA
                     VSIEAFQRGMRELGYTEGKNIVIDYRYGEGKEERYTSMAAELVALKPAVIVTWGTDVT
                     AVVKKVTTAIPIVFALADRPDILGLVGSLSRPGANVTGVTTLNFELTTKRLELLKETI
                     PGLARVAILGLQHPLIPITVKEAEGTARSLGVQLQSIELKSPRDIESMFNRSSKEQPG
                     ALLFLPSREIVYGPAAVALALKQRLPTIASNTAITETGGLMNYGPRWADLSFRAATYV
                     DKILNGAKPAELPIEQPTKFELVINLKTAKQIGLTIPPNVLARADRVIR"
     gene            2243..3343
                     /locus_tag="VF182_12905"
     CDS             2243..3343
                     /locus_tag="VF182_12905"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438029.1"
                     /translation="MTEVGGQKTDTTPQIAEIPYQGAECSWLFLCRICGSMLNSIGDL
                     RALISILFTLLFVLCPFLDVQSSNLQRIGYLSASSSSEATPRTQAFRQGLRELGYVEG
                     KNLAVEFRYADGNFDRIPNLISELIRLDVGIIVTAGPSLTGPVKQATTTIPIVMTNDS
                     DPVGSGFIRSLARPGGNITGLTGLARELSGKRLELLKEILPKLSRAAILGISGIPGNA
                     RALKETELTAASLGVQTQYLDIRAAKDIEPAFGAAVRWRADAVSVLNGPPHIQRLIPD
                     VAAKNRMPAMYFNPAIIERDAGLLSYGASLSDLDRRAAAYVDKILKGAKPGDLPVEQP
                     IKFEMVINLKTAKQIGLTIPPNVLARADKVIR"
     gene            3343..4314
                     /locus_tag="VF182_12910"
     CDS             3343..4314
                     /locus_tag="VF182_12910"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438030.1"
                     /translation="MPGKIVFLALIALILNQHFPAEAQQPQLYKIGWLAPRSAAAVID
                     RFRREIENLGYVEGKNVAIESRYAYENLERFPALAEELVRLKPDVILTRGTPETIALK
                     NATKTIPIVFYSVTDPVGSGLVASLARPGANITGFSSIESILAGKRLELLKETVPKLT
                     RVAVLWNPQDLSSSQQWKESQAAARELGLEVYSMAVSSGDKYENAFKEAIKTGSAALA
                     VIAGSLEDDNRNQILTLASRHQLPAIYPRVIFVDSGGLMSYGSDPSEPMRRAAVMVDK
                     ILKGAKPADLPVEQASKFVLIINLKTAKQIGLTIPQSVFARAHKVIR"
     gene            4357..5397
                     /locus_tag="VF182_12915"
     CDS             4357..5397
                     /locus_tag="VF182_12915"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438031.1"
                     /translation="MIERRWMRLPDFLSDNLRSRIQNRKLAGIVAVFFTLAMDGAVAQ
                     AQQPPKVPRVGVLFIGSRNQPHLDAFKQGLRERGYVEQKNIVLEYRYAEGNNDRLPQL
                     ADELVRDEVDVIVTTASVSALAARRATKTIPIVMTSGNPLESGLAASLAKPGGNVTGL
                     TVLSADLSGKRVELLTETFPKTTRIAALQSPQDFEAGAGYKETQEAAKAFSLRLHSVE
                     VQSANDFEPAFAEMNKARETALLVILSPMITFNSRRIVELALKYRLPGMYPTKQFAEE
                     GGLMAYGPLIPDLYRRAATYVDKILKGANPGELPVEQPTKFELVINLKSAKHIGVTIP
                     PSVLARADKVIK"
     gene            5457..7076
                     /locus_tag="VF182_12920"
     CDS             5457..7076
                     /locus_tag="VF182_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438032.1"
                     /translation="MKLNKLFLFGILVTLLCPPAAIAQEKAMEKLGQVNFPVTCTAAA
                     QKQFDQALAALHSFWYEEALRLFTAVAETDPGCAMAYWGIAMSIYYPLWVPPSQPTLQ
                     KGMNAIEKAKSIGAKTEREGAYIAAIESFYKDSDKLEHRVRTLAYEKAMEQIHRQYPS
                     DREAAIFYALALNATAPPTDKTYAQQRKAGEILEKVFAEQPDHPGVAHYIIHSYDNPS
                     TANRGLSAAQRYIKIAPSVPHAQHMPSHIFTRLGLWQESIQSNLGAVAASKEYAAKVH
                     PEAAYYEHLHALDYLAYAYLQGAQDHEAKRVLDELRAIKKVQPEAFQAAYAYAAIPAR
                     YALERRRWSEAAALTVQPSAFPWSRFQWAEAVTHFARAMGSARSGNVASSQKDIEKLE
                     SLQDALVKAKDSYWAKQVDIQRRVASAWYLRAENKNDEALQLMQSAADLEDSTDKHPV
                     TPAPIQPARELLGEMLLELGEPARALKEFEVSQRVEPNRFRGLSGAAKAAQESGNREK
                     ARLYYAKLISLCERADADRPELKEAKAFLSE"
     gene            7295..>7514
                     /locus_tag="VF182_12925"
     CDS             7295..>7514
                     /locus_tag="VF182_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438033.1"
                     /translation="MNKKFFLAAMIIFTVGSLGVVNAQPTAKIPRIGIIAPGKSPQLD
                     ALRHGLRDFGYTEGQNLLIDYRYVEGNRD"
BASE COUNT         1830 a         2027 c         2049 g         1608 t
ORIGIN      
        1 agcctttccc aaggatccag aatcaaaaat tcgtgcaggt tgctggcctc gttctcgcct
       61 tcttaacgtg tgccgctccg gctgaggcgc agcagcgatc aaccatacct aagattggtt
      121 acgtgtcagc gaccggcgac agctcgaacc agggagttta cgttgaggca ttgcgacggg
      181 gtttgaaaga actcggctat gttgagggca agaatattac cgttgaatat cgtggcgcga
      241 gaggagagac gggtcgtatt ccaagactcg tgcgcgaact cgtgcaactc aaggtcgatg
      301 tcctagttgc cccagttctg cctgcaatac gggcagcgaa ggaggccacc agaaccattc
      361 cccttatcat ggtgatttcc gaggatcctg tagcagcggg cttggtggat agcttagccc
      421 gtcccggtgg aaacatcacg gggctaacaa gacttcagcg tgaattgagc ggaaaacgat
      481 tggaactgct caagcagacc attccccgac tagcgcgcat cgcagttctt cgcgatgcag
      541 agagccccag cgcggtcatc gctttcaaag aatacgagaa tgcagcccat gtcttaaaac
      601 tacagcttca atccgtcggg gttctaggta gcaagcccga cttggacgga gcatttcaaa
      661 atgcagccgc tcaacgtgtg aatgcgttga tcactgtcac aaacgccaac atccttcgtg
      721 accaggagcg cgttgctgag ttggctttga ggcaccggct gccttcgatg ttcgagggaa
      781 gtacctgggt tgacgcaggt gggcttattt cctattcgac caacgatctt gaaatctacc
      841 gccgcgccgc cacttatgtg gataaaatcc tcaaaggcgc gaaaccttcc gagctacccg
      901 ttgagcagcc gaacaagttc gagctggtca tcaaccttcg aaccgccaag caaatcggat
      961 taaccattcc atccaatgtc ttggctagag cagatcgtgt ggttcgatga ttcctaatcc
     1021 aataattgaa atccaaaatc tagaaatcgt aaggattgcg ccgcacgtgc ttgggcctat
     1081 cgagttgttt tctggcgtgc gcctgcaagg gcggcgcagc acagaccaat gaaaccacgc
     1141 gcccgagaga tcggatcatt ctgaagaggg cgcgcgcctg caccgggaga cccatgagca
     1201 ttggggcaca agccattcgt ctaattctcg ttcttctgtc gttccctgct ttagcgctgg
     1261 cgcagccggt gcagcctgtc gtccgtgtcg gcgcgctcca tcttggcaca acaaagacgg
     1321 ccgcggtctc catcgaagct tttcagcgcg ggatgcgcga actgggctac actgaaggaa
     1381 agaatatcgt catcgattac cggtacggag aagggaagga agaaagatat acctccatgg
     1441 ccgctgaatt ggtggccctg aaaccggcgg tgatcgtgac ctgggggacc gatgtcaccg
     1501 cagtggtcaa gaaagttaca acggccattc caatcgtatt cgctttggcg gaccgtccgg
     1561 acattctcgg gctggtgggc agtctgtcgc gtccgggcgc aaacgtgacc ggtgtcacca
     1621 cgctgaattt tgagctcacg accaaacgtc tcgagctgct gaaggaaacg attccaggtc
     1681 tggctcgagt tgccatcctc gggctgcagc atccattgat tccgatcacc gtcaaagaag
     1741 cggaagggac ggcacgatca ctcggagtgc agctccaaag catcgaattg aagagccctc
     1801 gcgatatcga aagtatgttc aaccgctcca gcaaggagca accgggagct ttgctgtttc
     1861 ttcccagccg ggaaatcgtt tatggtccag cggccgtcgc cctcgcgctg aagcagcgcc
     1921 tgccgacgat cgcgagcaat accgcaatta ccgaaaccgg tgggctgatg aattacgggc
     1981 cgcggtgggc ggatttatcg tttcgcgccg cgacctatgt ggacaagatt ctgaacggag
     2041 ccaagcccgc ggaactgccg atcgagcagc cgacgaagtt cgagctggtc atcaatctca
     2101 aaaccgccaa gcagatcggc ctcaccattc ctccgaatgt tttagccaga gccgaccgag
     2161 ttatccgatg atcctccaat ctaaaatcca aatggtgagt gattccgccg aacgtgctgg
     2221 cgcgagcaga ccgggtgatc agatgacaga agtcggaggt cagaaaacag acaccacgcc
     2281 tcagatagca gaaatcccat accaaggcgc ggagtgctcc tggctgtttc tctgccgcat
     2341 ctgtggttcg atgctcaatt cgatcggcga tctccgagcc ctgatctcta ttttatttac
     2401 cctgctcttt gttctgtgcc ctttcctcga cgttcaatcc tcaaacctcc aacggattgg
     2461 atatctgagt gcttcatcgt cttctgaggc tacgccccgg actcaggcat tccgtcaggg
     2521 cttgcgcgag cttggctacg tggaaggaaa aaaccttgcc gttgagttcc gttatgccga
     2581 cggaaacttc gatcggattc caaatctcat ttcggaactg attcgccttg atgtaggtat
     2641 aatcgttacg gcaggtcctt ccttgaccgg gcccgtcaag caagcgacga cgacaatccc
     2701 gattgtcatg accaacgaca gtgaccctgt agggagcggg ttcatccgaa gtctggcgcg
     2761 tccgggcgga aacatcaccg gcctcactgg gcttgctcga gaattaagcg ggaagcgatt
     2821 ggagcttctc aaggaaattc tccccaaact ttcgcgcgcg gcaattctgg gaatttccgg
     2881 tatcccagga aatgcccggg cattgaaaga gacagagctt accgccgctt ctcttggtgt
     2941 acagactcaa tacttagaca tccgcgcggc aaaagatatc gagccggctt tcggagcagc
     3001 cgttagatgg cgtgctgatg cagtatcggt cttaaacggg ccgccgcata tccagcggtt
     3061 gatcccagat gtcgccgcaa aaaatcgcat gccggccatg tacttcaatc cggcaatcat
     3121 cgaacgcgac gcgggactct taagttacgg tgcgagtctc agtgatttgg acaggcgtgc
     3181 cgccgcttat gtcgacaaga ttctgaaggg cgcaaagccc ggcgacttgc cagtcgagca
     3241 gcccatcaag tttgagatgg ttatcaatct gaaaacggcg aagcaaatcg gtctcacgat
     3301 tccgcccaat gtgttagcac gagcggataa ggtgatcagg taatgcccgg aaaaatcgtt
     3361 tttttagccc ttatcgcgtt aatcctgaat cagcactttc ctgccgaagc gcagcagcca
     3421 caactttata agatcggatg gctcgctcca cgcagcgccg cagcagtcat agatcgattt
     3481 cggcgagaaa ttgagaacct cggatatgtc gaggggaaga acgtcgctat cgaatctcga
     3541 tatgcttacg aaaacttaga acggttccca gccttggccg aggagcttgt ccgcctcaag
     3601 ccagatgtca tcctgacgcg cggcacgcct gaaaccattg ccctaaaaaa cgcgaccaaa
     3661 acaataccaa ttgtgttcta tagcgtaact gatccggttg gctccggttt ggttgccagc
     3721 ttggcgcgcc cgggcgcgaa tatcactgga ttcagcagca ttgagtcaat cttagccggc
     3781 aagcgactgg agttactcaa agaaacggtt ccaaaactca cccgagttgc cgtgctctgg
     3841 aatccgcagg atctaagttc aagccagcaa tggaaagaga gccaagcggc ggcacgagaa
     3901 ctaggattgg aagtttactc tatggcggta agcagcggcg acaagtacga gaatgcgttt
     3961 aaagaagcga ttaaaacagg cagcgccgct ctggcggtaa tagccggttc attggaggat
     4021 gacaaccgga accaaatctt gacgttagca agcagacatc aactgccggc gatatatcct
     4081 cgggtaatct ttgttgatag cggcggccta atgtcgtacg ggtccgatcc tagtgaaccc
     4141 atgaggcgag cagcggtgat ggtcgataag atcctgaaag gcgccaagcc agccgactta
     4201 ccagtcgagc aagcctcaaa gtttgttctg ataatcaatt taaaaactgc gaagcagatc
     4261 ggcctcacga tacctcagag tgtgttcgcg cgggcgcaca aggtaatcag atgaattgtg
     4321 gatctagaat tgccgattgt cgattaaggg aggagcatga tcgagcgacg ttggatgcga
     4381 ttgccggatt tcttgtccga caatctaaga tctagaatcc aaaatcgaaa attggcgggg
     4441 attgttgctg tgttttttac actcgcaatg gatggggctg tggcccaggc gcagcagccg
     4501 cccaaagttc cccgcgtagg agttcttttc atcggctctc gcaaccaacc gcatctagat
     4561 gcgttcaaac agggcctgcg agaacgtgga tacgtggaac agaagaacat cgtccttgag
     4621 taccgctatg ctgaaggcaa taacgatcga ctgccccaac tcgctgacga attagtacgc
     4681 gacgaagtcg atgtcattgt cacgacagca tcggttagcg cgctggccgc tcggagagcg
     4741 acaaagacga taccgattgt catgaccagc ggtaatcctt tggaaagcgg cctcgccgcc
     4801 agcttggcca agccgggcgg aaacgtcacc ggtcttacgg tactctcggc tgatttgagc
     4861 ggtaagcggg ttgaactcct caccgagaca tttccaaaaa caacgcgtat cgcggccctg
     4921 cagagtccac aggactttga agccggcgcc ggctacaagg aaacacaaga ggcggcgaag
     4981 gcattttccc tgcgtttaca ttccgtagaa gtccaatcgg ccaatgattt tgagcctgca
     5041 ttcgctgaga tgaacaaggc tcgtgaaacc gctcttctgg tgattctgtc gcccatgatc
     5101 actttcaact cgcgccggat cgtcgaactg gcgcttaagt atcggttgcc ggggatgtac
     5161 ccgactaaac aatttgctga ggaaggcgga ctcatggcgt acggacccct aatccccgac
     5221 ttataccgcc gggccgcgac ctatgttgat aaaatcctca aaggagcgaa cccgggcgaa
     5281 ttgcctgtcg aacaaccaac taagttcgag ctggtcatca atctaaaatc cgcaaagcac
     5341 atcggggtga caattccgcc gagcgtgctg gcgcgggctg acaaggtgat caaataaaac
     5401 cgggtgaggg tgacgcgaga tgaaggtgct accgtaaaaa catgaaggag gcgtttatga
     5461 agctcaacaa actttttctg tttgggattc tcgtcacact tctttgcccg cccgcagcga
     5521 ttgcgcaaga aaaggcgatg gaaaaactgg gccaggtcaa ctttccggta acctgcaccg
     5581 cggcggcaca aaagcagttc gatcaggcgc tcgccgcctt acactcattt tggtacgagg
     5641 aagcgctaag actgtttacc gctgttgcgg aaactgatcc tggttgcgcg atggcctact
     5701 ggggaattgc catgagcatc tactatcctc tctgggtgcc gccgagtcag ccgacattgc
     5761 agaaagggat gaacgctatc gaaaaggcga agtcgatcgg cgccaaaacc gaacgtgaag
     5821 gcgcttatat tgcggcgata gagagctttt acaaggattc ggacaagctc gaacaccgag
     5881 tccggactct cgcatacgaa aaagcgatgg agcagatcca tcgccagtat ccaagcgaca
     5941 gagaagcggc gatcttctac gcactggcgc tcaacgccac ggcgcctcca acggacaaga
     6001 cttatgctca gcagcgaaag gccggtgaga ttcttgaaaa ggtctttgcg gagcagccgg
     6061 atcaccccgg cgtggctcat tacattatcc acagctacga taatccgtcc acggcaaacc
     6121 gcgggctttc tgccgcgcaa cgctacatca agatcgctcc ctcagttccg cacgcccagc
     6181 acatgccgtc tcacattttt acgcggctcg gtttgtggca agaatcgatt caatcaaatc
     6241 tcggggccgt ggccgcatcg aaagagtatg cagcgaaggt tcatcccgaa gccgcatact
     6301 atgagcatct tcatgccttg gattacctgg cctacgccta cctgcaagga gctcaggatc
     6361 acgaagccaa gcgcgttctt gacgaactgc gggcgatcaa aaaagtccag ccggaagcct
     6421 ttcaggcagc ttacgcctat gctgcgattc cggcgcgtta cgcgctcgag aggcgccgct
     6481 ggtctgaggc cgccgcgtta acggtgcaac caagcgcctt tccctggagc cgttttcagt
     6541 gggcggaagc tgttacacat ttcgcgcgcg ccatgggatc tgcgcgcagc ggcaatgttg
     6601 ctagcagtca aaaggacatc gaaaaactcg agtcgcttca ggatgcccta gtcaaggcta
     6661 aggacagcta ctgggcaaag caggtcgaca tccagcgccg ggtggcaagc gcttggtatc
     6721 tacgggccga aaacaagaat gatgaggcgc tacagctcat gcagtccgcg gcggacctcg
     6781 aggactcgac tgacaaacac ccggtcacgc cagcgccgat ccagcccgcg cgcgaacttt
     6841 tgggcgagat gctgcttgag ctcggcgagc cggcgcgagc attaaaagaa ttcgaggttt
     6901 cgcaacgagt ggaacctaat cgcttccgcg gtctctccgg cgcggcgaaa gccgctcagg
     6961 aatctgggaa ccgtgaaaaa gcgcgccttt actacgcaaa gctaatttcc ctctgtgaac
     7021 gcgccgatgc cgatcgcccc gaacttaaag aggccaaggc atttttgtcg gagtaaatga
     7081 actgaacttg aatcgtgatc tagccctctt tcaacaacct ggagctgaga cctgtgtgag
     7141 cgattttgcc cttttgcggt ggattacttt ctggcccgat cttcatcgaa tgaccaaaat
     7201 taaaactgaa aacagatccc tttgacagct ttgagagcgg ataaggttat tgatgacggg
     7261 ttatcgtaga tccgatgtcc gttcgagaga aatcatgaat aagaagtttt ttttggcagc
     7321 aatgatcatc ttcaccgttg gttctcttgg ggtcgtcaat gcgcagccga ccgcaaagat
     7381 tccaaggatt gggattatcg cgcccggcaa gtcaccgcag ctcgacgctc ttcgtcacgg
     7441 cttgcgtgat tttggctata ccgaagggca aaaccttctg atcgactacc gctatgtcga
     7501 aggaaatcgc gatc
//
LOCUS       DASUVS010000237         4048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_968763, whole genome shotgun sequence.
ACCESSION   DASUVS010000237 DASUVS010000000
VERSION     DASUVS010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968763"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1142)
                     /locus_tag="VF182_12930"
     CDS             complement(153..1142)
                     /locus_tag="VF182_12930"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438034.1"
                     /translation="MLPKTFSIMMAVALFTSPVRAEPIKVAYSGVSSAGTPLWLAKDA
                     GIFARHGLEADLITVRSAPLQVSALVSNEVQFVRGSASSMLSAAAQGAKLKILLSLFA
                     ERASYDFLVSSSITDPHDLRGKKIGVQDFSGLLWSLTMLALREMGLDPQKDNISIQAI
                     GDSTVIAQALATGLIDGAALDKLQSVRLQGLGVKVLLDLSRIALPSSPYMGAEAFIQK
                     NPQTVENFVKAMIEASTMMRAQKEKGLAVLQKHIKTDRQLAEIGYKLLLDDLTPYTFV
                     TVKGLKTVQEIVALRDPKIAKYNVEDLLDQRILEKVVKSGYVEEIERKYKIRP"
     gene            1842..2261
                     /locus_tag="VF182_12935"
     CDS             1842..2261
                     /locus_tag="VF182_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438035.1"
                     /translation="MDIIIEMRPESGCLHASAMGEFSLAEAKRTFLEMLEAVARHQVK
                     KVLFDGRRLTGDPETLERFYYGKFAADAVLQFGGRGVAPAIQFAYVLKEPVLDPERFG
                     ETVAVNRGMLVKTFDNLEDALGWLRITPANKRDGDDG"
     gene            2391..3536
                     /locus_tag="VF182_12940"
     CDS             2391..3536
                     /locus_tag="VF182_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6438036.1"
                     /translation="MNLIPPDSPDNPRISSGLLDVLIRAGLISALAVLCYQVFSPFLS
                     LMAWSIILAITMYPLHQWLARRVGGKQWLASTILVIIGLLLIIMPTGLLMNSFADSVR
                     HFIGAVENNTLSIPAPPERVEQWPIIGKKVYDTWSKAHADLPGLVQSLQPKIGELAHK
                     ALAVVASIGGDLLLFLGSFIIAGIVLAYGESGSRSTRAIFHRVAGVARGEALAKLCTS
                     TIRAVALGVIGIAFIQAILIGLALLLAGVPAAGVLSIIALVLGVAQVPALIVTLPVIV
                     YIWSSGDYSNSAAILNTIILLLTGMADNVLKPLMLGRGVDVPMPVILFGALGGMASRG
                     ILGMFVGATVLALGYEIFMGWVATTPASDPVVPQNEPIKPSDSRPNT"
BASE COUNT          898 a         1087 c         1088 g          975 t
ORIGIN      
        1 cgccatgaac cctaacaggc tgcggaaaaa gacccatcgg cttcgttgcg tttaatcgtc
       61 tcgctcaatc cgtgttcgtg gtacgcgtgt tcgtgttcgt tcggaattct cgatcacgct
      121 ttcacgcaca cgaacacaat cgtccggtgt cttcacggcc gaattttata ctttctctca
      181 atctcctcga cgtagccgct tttgacaact ttttcgagaa tgcgctgatc gagaaggtct
      241 tcaacgttat acttagctat tttgggatcc cgcagggcga cgatttcctg gaccgtcttc
      301 aatcccttga cggtaacgaa cgtataaggc gtcagatcgt cgaggagaag tttatatccg
      361 atttccgcga gctgcctgtc tgtcttgata tgtttctgca gaacggccag ccctttttct
      421 ttttgagcgc gcatcatcgt gctcgcctcg atcatcgcct tgacaaagtt ttctaccgtt
      481 tgaggattct tctggatgaa agcttccgca cccatgtagg ggctggaagg aagagcgatc
      541 cggctcaaat ccagtagcac tttaactccg agtccctgaa gccgcaccga ctgtaattta
      601 tccaacgcgg ctccgtcgat cagtcccgtt gccagagctt gggcgatcac ggtgctgtcg
      661 ccaatcgcct gaatactgat gttgtccttt tgaggatcca atcccatttc tcggagcgcc
      721 aacatggtaa ggctccagag aaggccactg aagtcctgaa ccccgatctt ttttcctctc
      781 agatcatggg gatcggtgat cgaagacgat accagaaaat cgtaagatgc tctttcggcg
      841 aacagcgaca agagaatttt gagcttcgcg ccctgcgccg cggcggaaag catactcgaa
      901 gcgctgccac ggacgaactg cacctcgttg gagaccagag cggaaacttg caaaggcgcg
      961 ctgcgcaccg tgataagatc cgcctctaat ccatgcctgg caaaaattcc cgcgtctttg
     1021 gcgagccaca gcggagtccc tgccgacgat acaccggagt aagcgacttt gatgggctcg
     1081 gccctgaccg gcgaggtaaa cagcgctacg gccatcatga tagagaatgt tttggggagc
     1141 atagtgatga tcctccgtga aatcgccgag agcatttcga ggagcgccaa catccgtcat
     1201 cagcttcacg aaacgtaaat caagcaacag atctgatccg tacatactcg tcagccctca
     1261 cggaatcaag aaatttcttg tcgatgttta catgatagaa ctgcgcgagg aaatccataa
     1321 agccaagtat taaatcgagg cggacatggg gtgcgtagcc tcgctgacag cttaaatttc
     1381 gtaagaaaat gctcagatgc gacgagttcg attatacgga agcggctttc ggtctttatt
     1441 tgattgggct actgataaac gatcggttgt cgcccagatt tcgttgctta attggctaac
     1501 gtattcggtt tctctggcta gggatttttc cggagaacat agaacgatta aaagaggaca
     1561 cttctttgat ccttataatc aacgaatttt acaactcggc gctaagccta acttgacgct
     1621 tcagaccgat cgctaagata gtcggctgag cgatgcgttc ggcggcggac atcacacggt
     1681 caatcaatct gcttcgacgg ccggtacgaa cacattttga tgaccagacc gaaatcggag
     1741 ggcgcgtaac attggacttg agacagatct tcggaccagt cgcgacgctc gcgcgccgcg
     1801 cctgctcagc tttaagcgtt gctggaaagg ctggtgacaa gatggacatt atcatagaaa
     1861 tgcgccctga atcagggtgc cttcacgcaa gcgcaatggg ggagttttcg ttggcggagg
     1921 cgaagcggac ctttctggaa atgctggagg cggttgcccg acaccaggtg aagaaagtac
     1981 tcttcgatgg ccggagactg acgggagatc ccgaaaccct agaacggttc tactatggca
     2041 aattcgcggc cgacgcagtc ttgcagttcg ggggtcgagg tgtggctcct gccatacaat
     2101 tcgcgtatgt gcttaaagaa cctgtgcttg accccgagag gtttggcgaa acggtcgcag
     2161 taaaccgggg tatgctggtc aagaccttcg acaatctcga agacgcgctc ggttggctga
     2221 gaataacccc agccaacaag cgggatggag acgacggcta acagccgcgc ccgattcaca
     2281 tcgttaagcc gcctcaagca aaataggtgg ggatgactaa tgcgactgtt gctcggcctt
     2341 cttacgtagt acgctacgat ggcaagctcc gccttcacta tagaaaacca atgaatctga
     2401 ttccgccaga ctccccagac aatccgcgaa tctcatccgg tctgttggac gtactgattc
     2461 gcgcgggttt gattagcgcc ctagcggtgt tgtgctacca agtcttttcg ccgttcctgt
     2521 cactgatggc ctggtcgatc atcttggcga tcacgatgta cccgttgcat caatggctcg
     2581 cccgtagggt cggcgggaag caatggctcg cctcgaccat tctggttatc atcggcttgt
     2641 tgttgatcat catgccaacg ggcttgctga tgaattcttt tgccgactcc gttcgtcatt
     2701 tcatcggcgc cgtggaaaac aacacgctat caatacctgc cccgccagag agagtcgagc
     2761 aatggccgat aatcggcaag aaggtctacg acacctggtc gaaagctcat gctgatctac
     2821 ctggactcgt gcagagcctg cagccgaaga tcggcgaatt ggcccacaaa gcacttgccg
     2881 tggtcgcaag catcggcggc gacctgttgc tgtttttggg ctcattcatt atcgccggta
     2941 tcgtattggc atacggggaa tcgggttcgc gcagcacccg agcgattttc catcgagtcg
     3001 ccggcgtagc aagaggcgag gcgctcgcca aactctgcac gtcaaccatt cgcgccgtcg
     3061 cgcttggtgt catcggcatc gccttcattc aagcgatcct catagggttg gcgctactgc
     3121 tcgccggcgt acctgcggcg ggtgtgttgt cgatcattgc tctcgtattg ggtgtcgccc
     3181 aagtgccggc gctgatcgtc acacttccag tcatcgttta catctggtcg agcggcgatt
     3241 acagcaactc ggccgcgatc ctaaacacga ttatcctgct gcttaccggc atggctgaca
     3301 acgttttgaa accgctcatg ctcggccgcg gcgttgatgt tcctatgccg gtgatcttgt
     3361 ttggagcttt gggcggtatg gcgagccgcg gaattctagg aatgtttgtc ggcgcaacgg
     3421 tgttggccct tggctacgag atcttcatgg gttgggttgc cacaaccccg gcatcggatc
     3481 cggtggtacc acagaacgag ccgatcaagc cgagcgattc gcgaccgaac acttaaaata
     3541 acgcctgata ttcggcagtc cgagcgccag cctaagcaag gcccgcacgg gagcgcgcgg
     3601 agggtttcgc atgaatccct gagcttgctc gcgcgccggg cactagacaa aacaaatgag
     3661 tccatccaat tcgtttaagt tagagtgccg gctcttgcgt tggcttggct gtttgggcct
     3721 actagttcgg atgatctttg acaagatcct ttttgctgct gtcattggcg gatcggggat
     3781 tgtccaatcc cggcgctgga gccggcacaa agttaagtga tggtcattgt tttcttgata
     3841 gcggctcagc ggcatcgtta ctccgccgaa aagtaggcag agaaatgccg agtttccgca
     3901 cagtgcccta tcactcgccg atgtcaatgc gattgtgctc agatcgcgct tcaaatattt
     3961 cgctgcttcc gtaaaactga tcccgcccac ttctcgtccg attgcagcgt aatcgacgct
     4021 tgaccacttc gctgagcaga gcctggaa
//
LOCUS       DASUVS010000238        35648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1191635, whole genome shotgun sequence.
ACCESSION   DASUVS010000238 DASUVS010000000
VERSION     DASUVS010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 35648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35648
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191635"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VF182_12945"
     CDS             <1..405
                     /locus_tag="VF182_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438037.1"
                     /translation="AILIRKRRPLQVVTAVALLMVALTVSGSLWILPYVNQFKSHRPF
                     SLEINRIVPPAAPLYIYADTMNDFNFYTGREIIPVLSSPGEVANLRRQTENNYLLIKD
                     RDLKSLRMPELGEVVARDTLGSTTWNLVSLNP"
     gene            complement(499..1335)
                     /locus_tag="VF182_12950"
     CDS             complement(499..1335)
                     /locus_tag="VF182_12950"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6438038.1"
                     /translation="MARILLVLFGFLFLISIWGFYISIRPPKIVSSMTPRALNLHYEN
                     VSFKTGDGLTLRGWHVPSGKKTDKTLILLHGYPADKGDILPAIAFLQEDFNLLLFDFR
                     YLGESEGSYSTAGAKEVDDLLAAIRFLKNRAITEVGVWGFSMGGAVALMAIEKAPEIR
                     AVVAESSYASLSDMASQFFRVRLINHPIAYLIGLWAKLFLGIDLRDASPAEKIRNSAI
                     PILITHSSSDSVVPFSQARLLQQALANNPHAEFWFTSELAHGQLDAEYKNRLQNFFRK
                     NL"
     gene            1550..2569
                     /locus_tag="VF182_12955"
     CDS             1550..2569
                     /locus_tag="VF182_12955"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6438039.1"
                     /translation="MKLLTRLEVVCLLVMIAVSAHAQEPFYKGKTVRVVVGFSAGGGF
                     DVYSRTISRHIGKHLPGNPTVIVENMTGAGSLVAANHVFKVAKPDGLTIGNINGGIFM
                     QQLLGWPGIEFDALKYEYVGVPVKDKSVCVMTKASGYTSFEKWMASKSPLKLGATGPG
                     SATHNVPLILKEALDLPIQLVSGYKGIADVRLAAEGGELAGVCGWTWDSLKATWTRAL
                     ESGDAIVVLQTVSEPIAELAKVPLAISAAKTEESRQLIQAGIHDVSDLTYSYVLPPGT
                     PKDRVEVVRKAFADTMKDAEFITDAKKSKLGVDPMTGAELEKTVGGLFKLAPGTVSKL
                     KDVLK"
     gene            2585..3091
                     /locus_tag="VF182_12960"
     CDS             2585..3091
                     /locus_tag="VF182_12960"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6438040.1"
                     /translation="MAEDKGPRLIVESMKEAGVSFVTSLPDANLQKLLALVDADPELT
                     HVTLAREEEGIGICAGAYLGGLTPAIIMQNGGFLNSCNALTTTALNFNIPILLLIYYA
                     GDLGDEGFSMLGSVTEPVLRGLGIRYQVLRRLDEIKRIIEGASILAIDSRRPVAVLLS
                     KDVLGMRK"
     gene            3088..3705
                     /locus_tag="VF182_12965"
     CDS             3088..3705
                     /locus_tag="VF182_12965"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6438041.1"
                     /translation="MKRYDCLKIAAEEMSDALVITTVGGAAAEWNTIRPSDGNLRCRT
                     LGLVSSIAMGLALALPKRRIVGIDGDGALLMNACGLATLAWQKPANLTLLLFDNSIYE
                     ASGLRPTATSVGTDLIGMAKAAGLKNAFWATSLEDYRNELRRAKHGSALSMIGAKTES
                     GMEYFKTWRNLPAFEYNEVENVYRFMRHIEKLEGKRVVPVPGPRH"
     gene            3890..4756
                     /locus_tag="VF182_12970"
     CDS             3890..4756
                     /locus_tag="VF182_12970"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6438042.1"
                     /translation="MSATEIPSEAIDNSYVHDGVRIHFRDRGAGSPIVLIHGFGASLD
                     TWRYLEDGLKNDYRVVSLDLKGHGYSDRPPDDRYSLQDHAAVVLGLMDHLKLKNVVLA
                     GNSLGCAIALLAALKAQEESSAKVAAMVLIAGSLDGDKLPLYLRLLRLPVIGSLATKL
                     TPATFGTRLILKRAYYDDEKVTDSLVELYAKYQRIPGTEHALITTARQMVPADISGLR
                     EALKNLEIPTLNIIGKHDQIISRESAERICQILPRCRAVTIEQAGHVPQEEKPEEVIP
                     LIKDFVAKVSDH"
     gene            complement(4824..5909)
                     /locus_tag="VF182_12975"
     CDS             complement(4824..5909)
                     /locus_tag="VF182_12975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6438043.1"
                     /translation="MAAALLTLCDVIVRYGETVALQIAGLELHTREVLAIIGANGAGK
                     STLLRVMGLLQRPSEGTVLFQGEDAFDGNLLAHRRRVATVFQEPLLLNSTVYENAALG
                     LKLRGAAGREIARRIGPWLERLGIEHLRGRNTRNLSGGEAQRTSLARALALEPELLLL
                     DEPFAALDPASREALLRDFQPILNENKITTVFVTHDRNEAFSIAGRVGVLHQGRLAQI
                     GAREDVFHRPASETVAEVVGIENRLPGVVEDCAGGLASIRVRQNRLRAPGQLKPGTRV
                     IVCLRPENIRISRDSCPADDSNRLTGVILKSVAAGMMHQRITLDCDGMLLVALLERRT
                     CVALKFAERERITIIFSFGAAHIIIAD"
     gene            complement(5891..6604)
                     /locus_tag="VF182_12980"
     CDS             complement(5891..6604)
                     /locus_tag="VF182_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6438044.1"
                     /translation="MEVIWHGFLKAAELVFALDAEVWSITGRSLQISGCATLISLLVG
                     IPLGIALALMKFPGRNMTVALINTGMGLPPVVVGLFISIFLWRSGPLGFLELIYTPTA
                     MIIAQVVIAFPIVAGLTMASFQALDPKLALQLLGIGASKPQLLWLLCKEAKLPLLAAV
                     MAGFGGVISEVGASMMVGGNIRGQTRVLTTATVLETGKGNFEIAIALGLILLALTFAI
                     NLLLTRIQQREQLRWPRLS"
     gene            complement(6606..7460)
                     /locus_tag="VF182_12985"
     CDS             complement(6606..7460)
                     /locus_tag="VF182_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX6438045.1"
                     /translation="MRIARQLLPLIILISGVLGGASSQSAEIKSRSVILSTTTSTQDS
                     GLLDVLVPLFEKQTGYSVRTISVGTGQALELAAKGDADVALVHAPSLEKKYIAAGKIK
                     NRRLVMYNDFVIIGPKDDPAKIASLKSAAAAMKAIEHAKAPFVSRGDNSGTHNLERSL
                     WKDAGIQASGSWYIESGQGMGATLGIAQERNAYTLTDRGTYLALQKRVTLPILVQGDR
                     TLLNIYSVMEVNPANGPRVYAAAGKAFADFMVSAEAQNLIKSFGVEKLGQPLFVPVAD
                     KREEELGA"
     gene            complement(7552..8655)
                     /locus_tag="VF182_12990"
     CDS             complement(7552..8655)
                     /locus_tag="VF182_12990"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438046.1"
                     /translation="MAEYRIIDADGHVMELDSELREFIGPPYKDLEWHQSYSFWPGLT
                     MDGYLRSLRQKGGWAGGGDGPKANHWLDFLDKNKIELTVLYPTQGLTHGAIQDRDWAI
                     CMARAYNDWLYHKFMAVSPRLVGVALLPIQDISEAVKELRRCVNDLGMVGAVLPAVAP
                     AGRLYSGPEFYPLWEEAQKLDIPISTHGGLSMPSLGLDMVTNFTIGHTLEHPFAQIRQ
                     FTALMFEGVFELFPNLRFGCIECGVGWVPYMMDRMDEEQERKGHYSPRCKLKPSEYVK
                     KGNIFFAVEVEESALPLAAQVANENALIWASDYPHERDQRDFSQDIPNLINRKDISDE
                     LKRKIFWDNPLKFYPRLRERVEKSQISKKAAGA"
     gene            complement(8832..9791)
                     /locus_tag="VF182_12995"
     CDS             complement(8832..9791)
                     /locus_tag="VF182_12995"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438047.1"
                     /translation="MAKLKLTLACDKYDYLQPLREGQVEVEGIDLNLITVESGIRHER
                     MFRYGEYDACEFSMSSYLVARGQDVDWLQAIPFFPRRMWGHKFCFIRAGSGIKKPADL
                     KGGRIGLRSYENTLALVTKGMLMNSYDLAVTDVTWVIVNKEHVGFKLPASIKVEFVDG
                     QRKLEDLLVEGKVDAEVEPDLPQRWIRNEGTVERLFPEFEKEEKQYYQRTKVFPIMHP
                     IVIKKEILDNDPWVATSLYEAFIASREAYNDFMEQPHRLSFAWARSYLEEERKFFGKD
                     PFYQGFRENRHDVQNLIKFAGQQGMLGRELTVEELFAENTHGT"
     gene            10085..10831
                     /locus_tag="VF182_13000"
     CDS             10085..10831
                     /locus_tag="VF182_13000"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014995188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX6438048.1"
                     /translation="MRFEDKSVIITGGGGKIGKAYAMAFAKEGAKVSLPDIASADHVV
                     KAIRDMGGTAISSACDVSDESSVKRMVEETAKQFGGVDILVNNAAYFMTVWKGPFWEM
                     AVDEFDKAMAVNVRGSWLCAKAAFPYMKKQGKGKIINISSGTVLNGSPNYIHYVTSKG
                     AIIAMTRAMARELGDYNICVNTVAPGFVETEGRQVEPAFSASRVANRSLKRVQVESDL
                     VGTVLFLASPESDFMTGQLLNVDGGANFTG"
     gene            10933..11676
                     /locus_tag="VF182_13005"
     CDS             10933..11676
                     /locus_tag="VF182_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6438049.1"
                     /translation="MRFKDKSVIITGGGGKIGKAYAVAFAKEGAKLSLPDIAGADHVV
                     KAVTDIGGSAISMACDVTDEKSVKAMVDETAKRFGTVDVLINNAAYFMTVWKGPFWEM
                     AVDEFDKAMAVNVRGSWLCAKAAVPYMQKQNKGKIINISSNVALTGNPNYIHYVTSKG
                     AIIAMTRAMARELGDFNINVNTVSPGFVVTEGRKVDPEYEKIRAEQRSIKRSQVEDDL
                     VGTVLFLASPDSDFMTGQLLNVDGGFHFI"
     gene            11786..12073
                     /locus_tag="VF182_13010"
     CDS             11786..12073
                     /locus_tag="VF182_13010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX6438050.1"
                     /translation="MFAVIKYQGFLNRGDKVASSDDQPGGEYRFEVGRRDPTAMLETS
                     VIGMREGGTREARVSPHLAYREGTRDRRIPPNAVIQWVIELVRVEDGQVKT"
     gene            12155..12643
                     /locus_tag="VF182_13015"
     CDS             12155..12643
                     /locus_tag="VF182_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438051.1"
                     /translation="MASKADFTTEEWKTIVAAPPMVGLAVTCASPNGPWGVLKEMLSM
                     GMAMAEMLQKGSSNALIAELAADLKARQTKPEPPQGIKDPEQAKELALNHVRMVNDIV
                     NRKATRDEADEFKKWLLAVGQRVAEASNEGGIFGFGGERVSDAEKNVLRQIAFALGLP
                     AS"
     gene            12854..13096
                     /locus_tag="VF182_13020"
     CDS             12854..13096
                     /locus_tag="VF182_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438052.1"
                     /translation="MARIKGVSESEANDEVRQVFEAQKKRLGIVSNTAKVYALRPTIQ
                     RGVQALAQGIQDSGLISPELKHLLCVKTANINGCPF"
     gene            13121..13444
                     /locus_tag="VF182_13025"
     CDS             13121..13444
                     /locus_tag="VF182_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438053.1"
                     /translation="MQNGASAEKVEAVLGDYKKNPLFSLRERLALELAERMTYTEERV
                     TDEFFDQLKEHFSDDELVELAAVIALENFRSKFNPVFGVEANNFCPLPQVKEAAAIAT
                     RRFHE"
     gene            complement(13639..16902)
                     /locus_tag="VF182_13030"
     CDS             complement(13639..16902)
                     /locus_tag="VF182_13030"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6438054.1"
                     /translation="MGDHRSAESNEERLSVNQILNTSELFRRSSRAPEYAAENRALIA
                     LAQAMKEAPRTFLQKLADIALELCHADSAGISILETHEGAGVFRWRAIAGHYAPNVNT
                     SMVRDACPCGTVLERDSVVLFDRPERHFVAFREMNLPIFENLSVPFHSGGEPAGTLWV
                     LTHSPERKFDAEDARLLTSLSRFASAAYAMIGTVEAAASNHTEENLPQSEAEFRAMFE
                     LSSVGQCQIHPASGRFLRVNRKMCEMTGYSPEELTLRTIVELTHPDDRDATALLLQQL
                     SRGEILVISIETRLLRKHGSTFWNHLNVSLLRRAAGQAPRATAIFRNVSARKETERKL
                     YEAEERMRLATQAAGVFAWDVDLLTGTIKYSENMAQLFGYETLPEKYFSVERARGVIH
                     PEDRERVIQASLPAFEQGGTFSAECRAESADGGYVWVHCNGTVVRDAYGNPARFVGIV
                     QKIEDRKRAEQALQASQERLGRAIQIETVGVIFFKTDGRIIEANDAFLRMSGYSRSDL
                     ESGLVRWDRMTPPEWMPRSLRGIEEFERMGRTVPYEKEYIRKDGSRWWALFAATRIEA
                     DEGVEFIIDVTESKLAEEKLRESEERFRSIADSSPVMIWVTDASGRIEFVNRSYLDFF
                     GTTRERAARLEWSEIIHSDDREYLDAFNVALRDRQPLHARARVKRHDNQWRWIESRGN
                     PRLAPSGEVTGYVGSSADITELIKSQEAMREADRRKDEFLATLAHELRNPLAPIRNSL
                     HILRLAGDSKPGTERTYEMMERQVNQMVRLVDDLLEISRITRGQIELRKERVLLTTII
                     QSAVEVSKPLIEEARHHLEISLPTAPLIFEADPVRLAQIVSNLLNNAAKYTNPGGRIW
                     LRAQREGDHLSISVRDSGIGISPDMLPRIFEMFTQLEHSTSRTQGGLGIGLTLVRSLI
                     EMHGGTIEARSQGPGKGSEFIVRLPLDDNLRTETRDNKQHAGQLPSKRVLVVDDNRDA
                     ADSLGKLLNLAGAEARAVYNGFAALEVLPTYQPAVLLVDIGMPDMDGYELARRVRQLP
                     DCEDLTLIALTGWGQAEDRQRSQAAGFDFHLTKPADFNALQTLLDSVQDRQRSHARH"
     gene            complement(17265..18278)
                     /locus_tag="VF182_13035"
     CDS             complement(17265..18278)
                     /locus_tag="VF182_13035"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438055.1"
                     /translation="MKRTRIIAASLTMLISLPSQFEISLAASAPLEKLTIGWSAVAGS
                     QAPFWITKEAGLFEKNGLDVVMIFLDGGSRATQAMMSGDLPIAQVGGNAPVVAKLRGA
                     DVTLISGLLNVLAYSMVVSPEIKRPEDLRGKKLTVSRFGSNSDYATRKILLKWGLKPD
                     IDVAILQIPGGQPSRLAAIQTKQVHGMVAQPPVTNMARKFNMNIIAQPEDFGGAYPTT
                     PVASRVSYLREKRDTARKFTRALLEGIHLYKTDKEFSKKVIGKYVKTSDNDALEDSYQ
                     FFSRLVPEKPYPSLDSVKEALAELGEKDAKARAARPEDFVDMSLIKEYDDNGFIDGLY
                     KKR"
     gene            complement(18462..19256)
                     /locus_tag="VF182_13040"
     CDS             complement(18462..19256)
                     /locus_tag="VF182_13040"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6438056.1"
                     /translation="MALLRYIAYLSDDPEKVSDFYRRFLGAEELGRSAEGDISITDGF
                     YNLTFFKRRAQLNEMKMECGLHHIGLAVDDLEETKGRFLALNPRGMILQEADDIHHGE
                     IRIHDPECFPVSLSTRRFGVPPEKEKKFPRIRHIAYNALDPEMMLRFYTQVFGLREVP
                     SSYQRRQQGRGNRFCGDGQTNLAIHPFYNPIEGHEAKFGINHIGFLTNTMEATLAELR
                     NVLNIAPRPPTRPYAEVRFRDPEGNALDLSQTKGWEVDVNKWVSAA"
     gene            complement(19312..20409)
                     /locus_tag="VF182_13045"
     CDS             complement(19312..20409)
                     /locus_tag="VF182_13045"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6438057.1"
                     /translation="MIFLTNAHVEQVLDMPTCIQAMEEAYHELNQLHAGYRPRIDFYV
                     PQEPHYYRWGTMEGASRKLGIFAIRMKSDMLAWENKDGFIVEDKYCIERGTYCGLIFL
                     TSTRNGEPLAIINDGYLQHMRVGACAGLGTKYLARQNAKVLAMIGSGGMARTYAEAIK
                     QVRPIEVMRVFSPTRVNREAYAAEMTEKLDMEVIAVDSPEKALRGADVVALTTDSLVP
                     VIKAEWLEPGMHINNVRNNEAGPEVLKRADVIARLGTSTLFADRNAPEVTTGSDGMFG
                     YIAGNIDEKNKIPSAPHYEIDNPEIGTVPDVMAGRWIGRANDQQITFLNNQGTQGLQF
                     AAVGGTAYNLARAKGLGHPLPLEWFTQNIRD"
     gene            complement(20418..21425)
                     /locus_tag="VF182_13050"
     CDS             complement(20418..21425)
                     /locus_tag="VF182_13050"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438058.1"
                     /translation="MKRYREVVILILAIARCSSSSPVTAQELEKLAIGHSNLRNDIAA
                     LWVPKELGIFRKNGLDANIVLITGGQRMTQALLSGSAPLGFTGATPVVTAVAGGAETV
                     MILGINNKLTYDIWAKPEIKKPEDLKGKSLAISSFGSSSHVASFLMLQHFKLDEKRDR
                     ITFLAIGDEPTRAQALIAGRIDATIIDPSVSLQIKDRGFTYLGNLEPLGVPFVNNALV
                     STRSFLKSKVRTAEAVVRSIVEGNAFMLNPANRARVTEILAKHLRLSGEEARRTYEDL
                     LPKVEARPYPNMDAVKATIEIMGSRNPKIAQLKPQDVVDTSILERLEQSGSIRSESGK
                     Q"
     gene            21749..22741
                     /locus_tag="VF182_13055"
     CDS             21749..22741
                     /locus_tag="VF182_13055"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438059.1"
                     /translation="MKTILTVVLALCWLALPPCSAAQEKFRAASGGFSTAIHALLWLA
                     YEKKIFQKYGLDGEYLALESGTPAMQALLANELQIVFTTGALAITANLQGADATIIAG
                     GINFIPNKLLVRPDIKTPEGLKGKRIAISRFGSASDYATQVALEKLGINPKDVTIVQV
                     GGNMTRLAALSQGTVHATLLSEPHTTVGMRDFKLNSLIDLAESGVPFPQNCFIVKRSY
                     FEANRPKIVNAMKAIIESYFILRKDKPLAVSLMKKYNRVSDEDASLGYDYYLAKYGDG
                     VMVLPDRKGLEFIISQVAATNAKAKGQTPESLRLLDGSVLEEIKKSGFIEKFKK"
     gene            22862..23953
                     /locus_tag="VF182_13060"
     CDS             22862..23953
                     /locus_tag="VF182_13060"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438060.1"
                     /translation="MAGVIDADTHIAESEAMWKFIDPEMYPRRPILAKIPDDTWYKDR
                     NAFWLIDGEIFPKPAGKASFSLITPSAAKREAGRGDIHLAVRELTDPIARLKDMDRIA
                     VEVQVIYPTLFLVYLTDDPQLETALCRAYNRFVADACAKAPERLKWVAILPLRAVDAA
                     LKEITWAKQHGAVGVFFRGMEGNLTLDNPYFFPVYGQAEQLDLPICIHTGSGSRYLMQ
                     LFDLERNHTFAHNRVLPVFAFRDLVHNRIPEKFPRLRFGFIEAAASWVPYIWHVLRRQ
                     QSNWRFSDPQELFREYRFWVACEADEELPHLVRFLGEDKLIIGSDYGHNDPSKEPQFV
                     ANLRAREDVPGALVEKILCENPRMLYGLS"
     gene            24133..25680
                     /locus_tag="VF182_13065"
     CDS             24133..25680
                     /locus_tag="VF182_13065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6438061.1"
                     /translation="MTNPSREYTILNVDDHDAGRYAKSRILQLAGYRVVEASTGADAL
                     RLVREAKPQLILLDVKLPDVNGIKLCRTIKSDPLSTHIMVLQISAVHTTRADRIYGLE
                     CGADTYLIEPVQADELLATVNALLRLYDRERENRQLVAQLRNADRQKDDFLAALAHEL
                     RNPLGVVSNAFDILPDTQTPEPVSHELRDIIGRQIGLMSRLVDDLLDVSRISRGQIGL
                     ACEPCDFAAIVRQTVDDYRAILESSGLELTLDVPSHPVWVVGDCTRLAQSISNLLQNA
                     GKFTKAGDVVSVKLVDIPDEHCAVLTIRDTGIGMESDFLARIFEPFSQFDPGIGRRQG
                     GLGLGLSLVKGLIELHGGEVHASSEGLGHGSEITIRLPTRMFAESPDAAPKTTIADAD
                     APASYRILVIDDNSLAARTLQIFLADKGHEVELAVTGPQGLEKARKFLPQVVLCDIGL
                     PGLDGYSVARQLRQEQGSDKIFLIAVSGYGQEEDQERAKSAGFDAYLVKPISLKDLET
                     LLAELNH"
     gene            26016..27314
                     /locus_tag="VF182_13070"
     CDS             26016..27314
                     /locus_tag="VF182_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEX6438062.1"
                     /translation="MLEPVITCPQCNIEIKLTESLAAPLIQSTRREFEMRLAQKDADV
                     AKKEAMLRDREAAIVRAKAQIDEELAAKLLQERGKIAAEEAHKAKLSLAADLEQKAKA
                     IAELQEVVKEREIKLGEAQKAQAEVLRKQRELDDLKREVELTIEKRVQESLTATREQA
                     RKETEEALRFKVLEKEQTISSMQRQIEDLKRRAEQGSQQLQGEVQEIELENLLRTKFP
                     RDSIQPVPKGEFGGDVLHRVNGSLGQSCGFILWECKRTKNWSDAWLAKLREDQRTAKA
                     EIAVIVSQALPKDIETFELVDGVWVTHPRAAMPVAAVLRQTLLEVAAVRQSSEGQQTK
                     MEMVYQYLTGQRFRQRVQAIVEAFSCMREDLDREKRAIIKQWAKREEQIDRVMQATVG
                     MYGDLQGIAGKTLQEIEGLEFPALDQPNIEQGDLWGRDKI"
     gene            complement(27311..27820)
                     /locus_tag="VF182_13075"
     CDS             complement(27311..27820)
                     /locus_tag="VF182_13075"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6438063.1"
                     /translation="MMKIKKILAPADMSSLCISGIRFALDLAQWQQSEVLIYNVLTVE
                     ETPFPQGTEEWVAKQTESPKLKKTLEQRKKMLDDFISKEFTELIGTTTVRQEVEIGTP
                     YRRIVDKAAAESVDLIVMSTHGRTGLAHMLIGSVTERVLRRAPCPILAVPAPRRTKRS
                     SKRLRKTAG"
     gene            complement(27892..28305)
                     /locus_tag="VF182_13080"
     CDS             complement(27892..28305)
                     /locus_tag="VF182_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013367211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEX6438064.1"
                     /translation="MKASISSFLIGSIFGFGLAISEMINPARVIGFLDVTGPWDPTLV
                     AVMGGALLVAAGLFPLVLRRNRAFLGDRLVLPTKTEMDTPLIVGAVIFGIGWGLAGLC
                     PGPALAGLASASPSLAGFVIAMIGGQWLVSKWEKI"
     gene            complement(28302..28724)
                     /locus_tag="VF182_13085"
     CDS             complement(28302..28724)
                     /locus_tag="VF182_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004346662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEX6438065.1"
                     /translation="MTEPNLVGGLIGGSLIGLAAVLLLAINGRIAGVSGILGGLLVPT
                     VKDDRAWRAAFLFGLIAGAALYSGWRGRVPLQLEVHGPVLAIAGLLVGIGTRLGSGCT
                     SGHGVCGLARRSPRSLLATLTFMAVAILTVYLTRHIFS"
     gene            complement(28743..29801)
                     /locus_tag="VF182_13090"
     CDS             complement(28743..29801)
                     /locus_tag="VF182_13090"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6438066.1"
                     /translation="MHRAGIKALVLLLAGFISEPAWPAQVIEPLKDALQLHEAGKIKE
                     AIVLYDRFIKTYPKRVEGYFNRGNAYYDLGQNQRAVKDYSEAISLAPKDSEAYFNRGN
                     AYRRLKQERAALDDYSAAIKLNPQDSRAYINRGSIHFDARRYDAAVEDFSAAIRLSPE
                     DADSYYNRGNSYLALNKTDEAIKDYTQALSLEEKLVDAFYNRGIAYSDSDKIDAAIAD
                     FTEVIKLSPKFSDAYYNRGLAAAKVGNYQAAVEDYSEAIRLNAKDPEAPFNRGIAYLR
                     LEKYDYAARDFTEVIRLQPRNAEAHVYRGIARVYQKKDNEADADFQKAFQLDPALKKT
                     VQPLIEEAKAQIKSEPAK"
     gene            complement(29807..30661)
                     /locus_tag="VF182_13095"
     CDS             complement(29807..30661)
                     /locus_tag="VF182_13095"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6438067.1"
                     /translation="MLPVFRIFFLFVALALPFGNIAAGGEAQNAFDQGVKHHQAGRYK
                     EAIAAYDRAIKASPDASEAYNNRGLAYHKLGQIGNAIKDYDQAVKLDPQFTDALYNRG
                     NALLTQKQYDRAIKDFDQVIKQNPKMPKAYDQRGQAYSGLGQYENAVKDFSQSQKLDP
                     KDPDLYYRRGLAYRRLGKHDLAVQDYSEAIKLEPKNAEAYNNRANAYSLLGQFNHALR
                     DYDQAIKLDPKNARIYANRGMVKLRQGKGEDAQTDFNKAMELEPSLKEQIQSITSDQK
                     AAPAVKRQ"
     gene            30843..31898
                     /locus_tag="VF182_13100"
     CDS             30843..31898
                     /locus_tag="VF182_13100"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438068.1"
                     /translation="MSIAAARTAQSVLADERIVSADSHIMEPENLWEKNLSPSLKTKF
                     PKFPPRNTAGEKPGGWNPKARLDEMAVDGVSAEVLYPTHGLRLFALEDAELQEACFKI
                     ANDWLIDYCAAAPGRLVGIPMISLYNIPNAIKELERCKKAGLVGSLIWQVPPEHLPFT
                     SDYYDPFWEASEALEMPPNLHILTGFNYSRFDRKGLDIYKATVNTKLYDAANTLFDIV
                     FSGVFARFPKLKIVYVENEVGWIPFFVHEWDKYFVRHSPKMPLPYMKKLPGEYVAEQV
                     YATFFSDPAGGRLMEDFGQDTFMWSNDYPHAASTWPHSRDVIARELGHLPKEILRKVV
                     RDNVIKLYNLKLDGINN"
     gene            31928..32419
                     /locus_tag="VF182_13105"
     CDS             31928..32419
                     /locus_tag="VF182_13105"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6438069.1"
                     /translation="MQASATAAKYEKDEKPQTKLFSLRTPYMKQGRITQLVAETENMW
                     IHTKINYEGGENEIHTHLDEDHSFIVLEGQMSVFDEKGNELKVGKYQGVMIPKGAYYR
                     YHNTGPDNLVVIRVGAGIKGKPQGGEDMRVRPDGKPLPAYSAENKTVEPIEDTRFFAD
                     FAG"
     gene            32588..33139
                     /locus_tag="VF182_13110"
     CDS             32588..33139
                     /locus_tag="VF182_13110"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX6438070.1"
                     /translation="MTPSKTTFTWETPPELWRGLKPSAAWKGSVVNLYIAPEPAGPMI
                     SVSEVRALADRGLEGDRFFRESWAAAKRPDKAVSLIEQEVLEMAESEQQLTIAGDKTR
                     RNIITRGVPLIELLDREFMIGNVVMRGIRLFEPCAHLEKVSRISGIFRALENRSGLKA
                     AILSDGVLRIGDPIALQAAKKAS"
     gene            33313..34260
                     /locus_tag="VF182_13115"
     CDS             33313..34260
                     /locus_tag="VF182_13115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX6438071.1"
                     /translation="MQFGLHLPASTAGVTSQDLVRFARRAEELGFYCITVADHIIVPK
                     NISVPYPYTADGKYPGAGYHLETLTTIGFLAGATTRIRFVTSVMIAPYRNPVITAKML
                     ASLDVLSHGRIIVGLGVGWMKEEFENLKTPPYRERGRVSDEYIHAFRELWTQDAPSFQ
                     GEYCSFSDIVFLPKPVQKPAIPIWIGGHSRQALKRAGQLGDGWHPIGGVPTIPLEPED
                     LKQDVEIMRGFAQAAGRDPDAIHVAFKGSLYDREKQIVPGKRRRFVGAVDDIIGDIHE
                     YRDAGVDTLIFDVRRPSAAGTLERMEWLAQEVIGKIEKA"
     gene            complement(35228..35521)
                     /locus_tag="VF182_13120"
     CDS             complement(35228..35521)
                     /locus_tag="VF182_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438072.1"
                     /translation="MTKFCYLAVMIAWVLWIRTQSPKADSWNALPGFQSREQCAANIK
                     EKLAVWRAFKDAVIGENTVTFTENNTTMTYICLTDADDPRRKSKPPALKEPVS"
BASE COUNT         8036 a         9499 c         9974 g         8139 t
ORIGIN      
        1 gcaattctaa ttcgaaaacg gcggccttta caggtggtca ccgccgtagc attgctgatg
       61 gtcgcgctca cggtatccgg ctcgctctgg atcctgccat acgtgaatca gttcaagtcg
      121 caccggccgt tttcactgga aatcaaccga atcgtcccgc cggctgcgcc gctctacatc
      181 tatgccgata ctatgaatga tttcaatttt tataccgggc gggaaatcat tcccgtgttg
      241 tcttcaccgg gggaggttgc caacctgcgc cggcaaaccg agaataatta tctcctgatt
      301 aaagaccgtg atctgaagag tctacgcatg cccgaattgg gagaagtggt ggctagggat
      361 acccttggca gcacgacctg gaatctcgtc tctttgaatc cgtgagggct aaagacgcgc
      421 cggatccctg gacataccgg ggttctactg cacggttgcc gctgctttat aatttccact
      481 gatcgtcgtg gcgttgtcct agagattttt ccgaaagaag ttttgtagcc ggtttttgta
      541 ttcggcatcc agctggccgt gcgccaactc ggaggtgaac cagaactccg cgtgaggatt
      601 gttagccaac gcctgctgca gcagtctcgc ttgagaaaac gggacaacag agtcgctgga
      661 cgaatgggtg atcagaatcg gaatcgcgct attacgaatt ttttctgccg gggaggcatc
      721 tcttaaatcg atgccgagaa aaagcttggc ccataaaccg ataagataag ctattggatg
      781 attgatcaag cgaactctaa agaactgcga agccatatcg gagagacttg cataactcga
      841 ttcggcaaca actgctctga tctccggagc cttttcgatc gccatcaaag ccaccgcgcc
      901 acccatcgag aaaccccata cccctacttc tgttatagct ctgtttttta aaaatcgaat
      961 cgccgccagc agatcgtcca cctctttggc tcccgccgtg gaataactgc cctcgctttc
     1021 accgagataa cgaaaatcga acaagagaag attgaaatct tcttgcagga aagcaatcgc
     1081 cggcagtatg tctcccttgt ccgccgggta gccgtgaagc agaatcaaag tcttgtcggt
     1141 ttttttcccg gagggcacgt gccaaccgcg taaggtaagt ccgtccccgg ttttgaagga
     1201 aacattttcg tagtgcaggt tcagggcgcg aggcgtcatc gatgaaacga tcttgggcgg
     1261 gcgaatcgaa atgtaaaacc cccaaatact gatgaggaac aaaaaaccga atagaacaag
     1321 aagaatgcgg gccatacttc taatcacttg tgcgggcatt ttcttcggca gttaaagcac
     1381 tgtcaacgtc atttgcaact cgagcagaac cgctcgcaca gcagatcggc gagcggagtg
     1441 cgtgcggctt agcgcggtcg attacttgcg tgattgacag cacctccgac ctcgcataat
     1501 tttacgtgag ttgtgaacgg tcctaatgct ggttttctaa aggaagacta tgaagctcct
     1561 cacccggttg gaggttgttt gtctgttggt tatgatcgcg gtctctgccc atgcgcagga
     1621 gcctttctac aagggaaaaa ccgtgcgtgt cgtcgtgggt ttcagcgccg gcggaggttt
     1681 tgacgtttat agcaggacga tctcgcggca tataggcaag catctccctg gcaatccaac
     1741 cgtcattgtc gaaaacatga ccggcgcggg tagtctggtg gccgctaatc atgttttcaa
     1801 ggtcgccaag cctgacggtc ttaccatcgg caacatcaac ggcggcatct tcatgcagca
     1861 gctcctcggt tggcccggga tcgagttcga tgctctcaag tatgagtacg tcggcgttcc
     1921 ggttaaggat aagtctgttt gcgtcatgac caaagccagt ggttatacca gttttgaaaa
     1981 atggatggca tccaagtcgc cgctaaaact cggcgccacc ggaccgggtt cggcgacaca
     2041 caacgtgccg ttgattctaa aagaagcttt ggatctgccc attcaactgg tctccggtta
     2101 taaaggcatt gccgacgttc gtttggccgc cgaaggcggg gagttggctg gggtttgtgg
     2161 ctggacgtgg gattcactga aggcaacgtg gacgagggcg ttggagtcgg gggatgccat
     2221 cgtcgtgctt caaacggtat ccgagccgat cgcggagcta gcgaaggtcc cattggcgat
     2281 cagcgctgca aagaccgagg agtcgcgcca gctgatccag gcgggcatcc acgatgtttc
     2341 cgatctgacc tattcgtatg tcttgccgcc ggggacgccg aaagatcgcg tcgaggtcgt
     2401 gcgtaaagct ttcgccgata cgatgaagga tgcggaattt atcaccgatg ccaaaaagtc
     2461 taaattgggt gtggacccta tgaccggcgc tgaattggaa aaaacggttg gtggactttt
     2521 caaactcgct cccgggaccg tcagtaagct taaggacgtt cttaaataga gcgcaaagat
     2581 ctctatggcc gaagacaagg gacctcgatt gatcgtcgaa agcatgaaag aagccggtgt
     2641 cagcttcgtc acatcgctgc cggacgccaa tctgcagaaa cttctggcgc ttgtggatgc
     2701 cgacccggaa ctgactcatg tgacgttggc gcgagaagaa gaagggattg gcatctgcgc
     2761 cggcgcatac ttgggcggtc tgacgccggc gatcattatg cagaacggcg gctttctcaa
     2821 cagctgtaat gcgctgacga cgacggcgct taatttcaat attccgatcc tgctgttgat
     2881 ttactatgcc ggtgatctcg gtgatgaagg cttttctatg ctcggctcgg tcaccgagcc
     2941 ggtgcttaga ggccttggaa tacgctacca ggtgctgcgc cgtctcgacg agatcaagag
     3001 aatcatcgaa ggcgcgagca ttttggcgat cgattcgaga agaccggtgg cggtactttt
     3061 gagcaaagat gttctcggca tgagaaaatg aaaagatacg attgcctgaa aatcgccgct
     3121 gaagaaatgt ccgatgcgct ggtgataacc acagtcggcg gcgcagcggc tgagtggaat
     3181 acgattaggc ctagcgacgg caacttacgt tgccgcacgc tgggattggt atcttctatt
     3241 gccatgggtc tggcgttggc cttgccgaag cggcgcatcg ttggcatcga cggcgatggc
     3301 gcgctgctaa tgaacgcctg tgggctcgcc actctggcgt ggcagaagcc cgcgaatctc
     3361 acgttactgc tgtttgataa cagcatttat gaagcctccg gtctcaggcc gaccgcgacc
     3421 agcgtgggca cggatctgat cggcatggct aaagccgccg ggttgaaaaa cgccttctgg
     3481 gcaacttcgc tggaggacta tcgaaacgag ctgcgcaggg cgaaacacgg tagcgcgcta
     3541 tccatgatcg gagccaagac cgaatcgggc atggaatatt tcaagacctg gcgtaacctg
     3601 ccggctttcg aatacaacga agtagaaaat gtctaccggt tcatgcgtca catcgaaaaa
     3661 ctagaaggta aacgggtcgt gcccgttccg ggccccagac attgaacggg ttgcgcgtta
     3721 ggcatctgca gattatcgca cacttgttaa tgggaccctt gagggccgct cttttcttac
     3781 ccgaaatgcc acccgaaacg tcgatggtat accggatctg atgcaatctc tctgcagcgg
     3841 gtatggcatt ttgttggtcg tcattggcct gttaggcagc tgttcgtcca tgtccgccac
     3901 tgagatccca agcgaagcca tcgacaacag ctacgttcat gacggcgtta gaatccattt
     3961 cagagatcgc ggcgcgggaa gcccaatcgt cttgatccac gggttcggcg cttcgctcga
     4021 tacctggcgc tatctcgagg atggtctcaa gaacgattac cgggtggtct ctctagatct
     4081 caaaggtcat ggttattccg acagacctcc cgatgatcgc tattctttgc aagatcatgc
     4141 cgcagtcgtg ctagggctga tggatcatct gaagcttaag aatgtagtgc tcgcgggcaa
     4201 ttcgttgggt tgcgccattg ctctactagc ggctttaaaa gcgcaagaag aatcttcagc
     4261 gaaagtcgcg gcgatggtcc tgatcgcggg cagcctcgac ggggataagc tgcctttgta
     4321 cctgcgactt ttgcggctcc cggtgattgg ttcgcttgcc acaaaactta cgccggctac
     4381 ttttggaact cggcttatac tcaaacgcgc ctattatgat gatgagaagg tcaccgactc
     4441 gctggtagag ctctatgcta aataccagcg gataccggga accgaacacg cgctcatcac
     4501 aaccgctcga cagatggttc cagcggatat ttcaggcctg cgcgaggcgc tcaaaaatct
     4561 ggaaattccg acactgaata tcatcggcaa acacgatcaa atcatttccc gggaaagcgc
     4621 cgagcgcata tgccagatct tgccacgctg cagagccgtt acaatcgaac aggccggtca
     4681 tgtgcctcaa gaagaaaagc ccgaagaagt cattccgctg ataaaagatt tcgttgctaa
     4741 agtctccgat cattgatttc gttttgtgaa ttgccccggc tagatccaca cttagacgct
     4801 gagacttggc gtcgatatcg tgatcagtca gcgatgatga tatgagctgc gccgaagctg
     4861 aagatgatgg taatcctttc tcgctccgca aacttcaagg caacgcaagt gcgtcgttct
     4921 aacagcgcca caagcagcat tccatcgcag tccaaagtga ttcgttgatg catcataccg
     4981 gccgcgacgc ttttgaggat tacgcctgtg agtcggttgg agtcatctgc cgggcagctg
     5041 tcccggctga tccggatgtt ctccggtcgt aggcagacga ttacccgggt gcctggcttt
     5101 aattgtcccg gggctcttaa ccggttctgt ctgacgcgaa tgctggccaa gccgccggcg
     5161 caatcttcga ccacaccggg cagacgattt tcaatcccca cgacctcggc cacggtctcg
     5221 ctggcaggcc ggtgaaagac atcctcgcgc gcgccgattt gagctaaccg tccctgatgt
     5281 aaaacaccga cgcgaccggc gatgctgaac gcctcgttgc gatcgtgggt gacgaagacg
     5341 gtggttatct tgttctcgtt cagaatcggt tggaaatcgc ggagcaaagc ttcgcggctg
     5401 gcgggatcca acgcggcgaa aggttcgtcg agcaacagaa gctcaggttc gagcgcaagc
     5461 gcgcgggcga gactggtgcg ttgcgcttca ccgccggaca agttgcgggt gttgcgtccg
     5521 cgcaaatgct cgatgccaag ccgttccagc cagggaccga tgcggcgcgc gatttcccgg
     5581 ccggcagcgc cacgcagctt taagccgaga gcggcgtttt catatacggt cgaattgagc
     5641 agcagcggct cctgaaaaac cgtagcgacg cgacgcctgt gagcgagcaa atttccgtcg
     5701 aacgcatctt cgccttgaaa gagcaccgtg ccttcgctcg gccgctggag caagcccatg
     5761 actcgcaaca aagtggactt gccggcgccg ttggcgccga taatcgccag aacttcacgg
     5821 gtgtgtaatt cgagaccggc aatctgcagc gcgaccgttt cgccataacg gacaattacg
     5881 tcgcacaatg tcaggagagc cgcggccatc gcaactgttc ccgctgttgg atgcgggtga
     5941 gtaagaggtt gatcgcgaag gtgagcgcca gcaagatcaa tcctagcgca atagcgattt
     6001 cgaagttgcc tttgccggtt tccagcaccg tcgcggtggt gagaacgcgg gtttgaccgc
     6061 gtatgttgcc gccgaccatc atcgaggcgc cgacttccga gatgacacca ccgaaccctg
     6121 ccatgaccgc cgccagcagc ggcagcttcg cctctttgca gagtagccag agcaactgcg
     6181 gctttgaggc gccgatgcca agtagttgca aggcaagctt gggatcgaga gcctgaaagc
     6241 tggccatcgt caatcccgcc acgatcggaa aagcgatgac aacctgggcg atgatcattg
     6301 ccgttggcgt gtaaattaat tcgagaaagc ccagaggacc gctgcgccat aggaaaatcg
     6361 agataaacag gccgactacc acaggcggca gacccattcc ggtattgatc agagctacgg
     6421 tcatattgcg cccgggaaat ttcataagcg cgagagcgat gccgagcgga atgccgacta
     6481 aaagcgaaat caacgttgcg cagccggaga tctggagcga acgcccggta atcgaccata
     6541 cttccgcatc cagggcaaag accagctccg cagccttgag aaagccgtgc cagatcactt
     6601 ccatctcatg cccccagctc ttcttcccgc ttatcggcaa cgggcacaaa caaaggttgg
     6661 ccgagctttt cgacgccaaa actcttgata aggttctgtg cttctgccga gaccatgaaa
     6721 tcagcgaaag ccttgccggc ggctgcataa acgcgcggtc cgttcgcagg attcacttcc
     6781 atcacggaat agatgttgag caacgtgcga tcgccctgaa cgagaatcgg caatgtgaca
     6841 cgcttttgca gcgccagata agtgccgcgg tcggtcaggg tataggcgtt gcgttcctgc
     6901 gcgatgccga gagttgcgcc catcccctgg cccgactcga tataccacga accgctggcc
     6961 tgaatgccgg cgtctttcca taaagacctt tcaagattat gcgtccccga gttgtcgccg
     7021 cgagagacaa acggcgcttt ggcatgctcg atcgctttca ttgccgccgc ggccgatttg
     7081 agcgaagcaa ttttcgccgg atcatctttg ggtccgatga tgacgaagtc gttgtacatc
     7141 accagccgac ggtttttgat tttgcccgcc gcgatatact ttttttccag gctcggcgca
     7201 tggaccagtg ccacgtcggc atcgcccttg gccgcgagct ccagcgcctg gccggtgcca
     7261 acagagattg tcctcacgct gtagccggtt tgcttttcaa acagcggcac caacacatcg
     7321 agcaagcccg agtcctgtgt gctggtagtg gttgatagga tgacgctgcg actcttgatt
     7381 tccgcgcttt ggctcgaggc cccgcccagc actccgctta tgagaatgat cagtggcaac
     7441 aactgtcgtg ctattctcat tgccgtctcc tgtgccggtg atcagttcat gtcgaaattc
     7501 gacccgcctc gctgcgatcc taccagccat ggcttccagt tatgtaatag ttcaggcgcc
     7561 ggcggctttc ttcgatattt ggctcttctc cacccgttcc cgcaaacggg gatagaattt
     7621 caaaggattg tcccagaaga tcttccgctt gagctcatcg ctgatatctt tgcggttgat
     7681 cagatttggg atgtcctggc tgaagtcgcg ctgatcgcgt tcgtgcggat aatcggaggc
     7741 ccagatgagg gcattttcat tggcgacttg cgcggctagc ggtagagccg actcttcgac
     7801 ttccacggcg aagaagatat ttcctttctt gacgtactcg ctgggtttta gtttgcagcg
     7861 cggcgaatag tgacctttac gctcctgttc ctcgtccatt cggtccatca tgtaaggcac
     7921 ccagccgacg ccgcattcga tgcagccgaa gcgcaagttt gggaacagct cgaaaactcc
     7981 ttcgaacatc aaggcagtga actggcgaat ctgcgcgaag ggatgttcga gggtatgtcc
     8041 gatggtaaag ttggtcacca tgtcgaggcc gagcgacggc atgcttagcc cgccgtgagt
     8101 cgaaatggga atatcaagtt tttgcgcttc ttcccaaagc gggtaaaatt caggaccgct
     8161 gtagagccgt ccggcgggag cgaccgcagg cagcacggcg ccgaccattc cgaggtcgtt
     8221 aacgcaacgg cgcagttctt taaccgcttc ggaaatatct tgtatcggca aaagcgcgac
     8281 gccgaccagg cgcgggctca cggccatgaa tttatggtag agccagtcgt tgtaagctct
     8341 cgccatgcaa atggcccaat ccctatcctg gatggcgccg tgcgtgaggc cctgcgtcgg
     8401 gtagagaacc gttagctcaa tcttgttctt gtcgagaaag tcgagccagt gatttgcctt
     8461 ggggccgtcg ccgccgccgg cccagccgcc tttctggcgc aaggagcgga gatagccgtc
     8521 catggtaagg cccggccaga acgaatagct ctggtgccat tctagatcct tgtaaggcgg
     8581 gccgatgaat tcgcgcagct cgctgtcaag ctccatgacg tgtccgtcgg catcgatgat
     8641 tcgatactca gccatattgc ctcctgaatc ggtttttact ttttactgtt ggccctttgg
     8701 aataaaggtc gagtgaccga aagatttctt gcttggctcg aacaggcaac gtacagccca
     8761 taatggttta cgcaagctga catgtgattc cgagaagccg ttcgataagg accctctctt
     8821 attcaggagc atcacgtgcc gtgggtgttc tcggcaaaca gctcttcaac cgtcaattca
     8881 cggccgagca tgccctgctg accggcgaac ttgatcagat tctgcacgtc gtgacggttt
     8941 tctctgaagc cctgatagaa ggggtcttta ccgaaaaatt ttcgttcttc ttccaaatac
     9001 gagcgtgccc aggcaaaact gagccggtgc ggttgctcca taaaatcgtt gtacgcttca
     9061 cgcgacgcga taaatgcctc gtacaggctg gtggccaccc acggatcgtt gtcgaggatt
     9121 tctttcttga ttacaatcgg gtgcatgatc ggaaaaactt tggtccgctg gtaatactgt
     9181 ttctcctctt tctcgaattc cggaaacagt cgctcgacag ttccttcgtt gcgaatccat
     9241 ctctgcggca gatcgggttc gacctccgca tcgaccttgc cctcgaccaa taaatcttcc
     9301 agcttccgtt ggccgtcgac gaattcgact ttgatgctcg cgggaagttt aaagccgaca
     9361 tgctctttat tgacaattac ccaggtgaca tccgtgaccg ccagatcgta gctgttcatc
     9421 agcatgcctt tggtgaccag ggccagggta ttttcgtagc tgcgcaagcc gatgcggccg
     9481 cctttcaaat ccgccggctt cttgatgccg gaaccggcgc gaatgaaaca gaacttatgc
     9541 ccccacatgc ggcgcgggaa aaacggaatg gcttgcaacc aatccacgtc ctggccgcgc
     9601 gcgaccagat aggaagacat tgagaactca caggcatcgt actcgccgta tcgaaacatg
     9661 cgctcatggc ggatgccgct ttcaaccgtg atcaggttta gatcgatgcc ttcgacttca
     9721 acctgtccct cgcgcaaagg ctggagatag tcgtatttgt cgcatgccag tgtcaatttt
     9781 aatttcgcca tatgatctcc tgggcgctga aagagcttca gacgcttccc ttgtgctcgc
     9841 cgattctaaa ttcgtgctcg tgtttacgtg ttcgtgttgg ttcggaaaaa tctcatcatg
     9901 ttcaccatca cgaacatggc tttatctgct tcgatcatag gtgcggcgcc acgtcaagtc
     9961 aatgaaacgc caggttcgtt gacaggcatg ctaaagctga atatgaaagc tgaaagcgga
    10021 atgatttttg gagagggctt tgttcagctt tccgctttca tcttttctaa agggggagtc
    10081 gaccatgcgt ttcgaagaca aatccgtgat catcaccggc ggcggtggca agatcggcaa
    10141 agcttatgca atggcattcg ccaaagaggg tgccaaggtt tcattgccgg atatcgccag
    10201 cgccgatcat gtggtcaaag cgattcgaga catgggcggc acggcgatca gctcggcatg
    10261 cgatgtgtcg gatgaaagca gcgtcaagag aatggtggag gaaaccgcca agcagtttgg
    10321 cggggtcgat attctggtga acaatgccgc ttattttatg acagtatgga aagggccgtt
    10381 ctgggagatg gcggtggatg aattcgacaa ggccatggcc gtgaatgtgc ggggatcttg
    10441 gctatgcgcc aaggcggcgt ttccatatat gaagaagcaa gggaagggca aaatcatcaa
    10501 tatttcctcc ggcactgttt tgaacggcag tccgaattac attcattacg ttacttccaa
    10561 gggagccatc atcgcgatga cccgcgccat ggcgcgggag ctgggcgact ataacatctg
    10621 cgttaacacc gtcgctccgg gttttgtcga gaccgaaggc cgccaggtcg agcctgcgtt
    10681 tagtgccagc cgcgtggcaa accgctcgct gaaacgtgtc caggttgaaa gtgatttggt
    10741 gggaaccgtt ctgtttttgg cctcacccga aagcgatttc atgaccggac aattgctgaa
    10801 cgtcgatggc ggcgccaatt ttaccgggta aacaagccgg aattatgaaa gctgaaagcg
    10861 gaaagaatcc gcctccaaag gcatctgttt caggtttccg tttcatcttt catcatttcc
    10921 ggtaagagag ctatgcggtt caaagacaaa tccgtgatca tcaccggcgg cggtggcaag
    10981 atcggcaaag cctatgcagt agccttcgct aaagaaggcg cgaaactctc attgccggac
    11041 atcgcaggcg ccgatcatgt ggtaaaagcg gtaaccgata tcggcggcag cgcgatcagc
    11101 atggcctgcg atgtaaccga tgagaagagc gtcaaggcga tggtcgacga gaccgccaaa
    11161 cgatttggta cggtcgatgt tctcatcaat aacgcggctt acttcatgac cgtgtggaaa
    11221 gggccgttct gggagatggc ggtcgatgaa ttcgacaaag cgatggcggt caacgtgcgc
    11281 ggttcctggt tgtgcgccaa agctgcggtg ccttacatgc agaagcaaaa caagggcaaa
    11341 atcatcaata tctcttccaa cgttgctctc accggcaatc cgaactatat ccattacgtt
    11401 acttccaagg gcgcgatcat cgccatgact cgcgcgatgg cgcgggaact cggcgatttc
    11461 aacatcaacg tgaacacggt cagtccggga ttcgtcgtca ccgaagggcg caaggtcgat
    11521 cccgaatacg aaaagattcg cgccgagcaa cgcagtatca agcgctcaca ggtcgaggac
    11581 gacctggtcg gcaccgtttt gtttttggca tcgccggaca gcgacttcat gacgggacag
    11641 ctgttgaatg tcgacggcgg gtttcatttt atttgatgcg agcgagattt ctgctgcagg
    11701 ctctctcttg gtgagcgtaa ggcaaaagaa gggcattagg ataaaggatc ttactcttgg
    11761 cattgggcga gtggcggagc ctggaatgtt cgcggtgatc aagtaccagg gttttctaaa
    11821 tcgaggagat aaagttgcca gctctgatga tcaacctggc ggtgagtatc gatttgaagt
    11881 cggacgccgt gatcccaccg ccatgcttga gaccagcgtt atcggaatgc gcgaaggcgg
    11941 aacccgggag gcgcgcgtga gtccgcacct tgcctatcga gaaggaacgc gcgaccgcag
    12001 gatccctccg aacgcggtta tacaatgggt gatcgagctg gttcgggtgg aggatgggca
    12061 ggtaaaaacg tagtatcatt cacaccggtt agggatttat cggcgcatca tttcagcgcg
    12121 acaaccattt attctcagag caggaggcat tccaatggcc agcaaagctg attttactac
    12181 cgaagagtgg aaaacgattg ttgccgctcc gccaatggta gggctagcgg tcacctgtgc
    12241 cagccccaac ggaccgtggg gggtgttgaa ggagatgtta tcgatgggaa tggcgatggc
    12301 tgagatgctg caaaagggca gttccaatgc gctcattgcc gaactggctg ccgacttaaa
    12361 ggcgcgtcaa accaaaccgg agcctcctca gggcatcaaa gatccggagc aggccaagga
    12421 attggcgttg aatcacgtga gaatggtgaa tgatatcgtt aaccgcaagg ccacacgaga
    12481 tgaagccgat gagtttaaaa aatggctgct ggccgtcggc caacgcgttg ccgaagcgtc
    12541 gaacgaaggc ggtatcttcg gattcggcgg cgagcgcgtt tcagacgctg agaaaaacgt
    12601 gctaaggcag atcgcctttg ccttgggact gccggcgagt tgacctgtcg caatcaaaga
    12661 cgctaaggct gccgatgtgt gttgtagttc taagtgtcat tcccgaacca ggggaacctt
    12721 ccagcgcaag ggatgatagt ttgctccacc gctacagcag tgcagctaaa ccgggcgacg
    12781 cgagatctac acggttatag aagccgatcg aattccgtgt aaaaatataa gttgacaata
    12841 aggagagtgg aacatggcta gaatcaaagg tgtaagcgaa agtgaagcaa atgatgaggt
    12901 gcggcaagtt tttgaagcgc agaagaagcg cctagggatc gtttcgaaca ctgcaaaagt
    12961 ctatgcgttg agaccgacaa ttcagagagg agttcaggcg ctggcccaag ggattcagga
    13021 ttcggggctc atttcgcccg agttgaagca tctcctttgt gttaagaccg ccaatatcaa
    13081 cggctgccct ttttgaattg acagcaatgc atccagtgcg atgcaaaatg gggcttcagc
    13141 cgagaaagtc gaagcagtcc tcggagacta caagaagaat ccgctgtttt cattgcgtga
    13201 gcggttggcc ttggaattag ccgagcggat gacctacacg gaagagcggg ttacggatga
    13261 gttcttcgat cagctcaagg aacacttcag cgatgacgag ttggtggaat tagcggccgt
    13321 cattgctctg gaaaattttc gcagcaagtt caatccggtc tttggagtgg aggcgaataa
    13381 tttctgcccg ctaccgcagg ttaaagaagc agccgctatc gcgactcgcc gattccatga
    13441 atgaaggcgg cgcgagtgat tcgcgttgcg cgaagttacc acaaggtaac cacaagaact
    13501 tgcacgaccc ggcagaatcc taagacccta taattcttta ggagctagtt tgctcagcgg
    13561 cagctttaaa ctgacaagcg ccggaactaa cgctggtggg tggagcaagc gcgacgcggt
    13621 gcgggccttg ggctcggctc aatgtctcgc atgacttctt tgccgatctt gcaccgaatc
    13681 cagcaacgtc tgcagagcat tgaagtcggc aggtttggtg agatggaaat cgaagcccgc
    13741 ggcctgtgag cgctggcgat cctcggcctg gccccagcct gttaatgcaa tcagcgtcag
    13801 atcttcacag tccgggagct gtcgaacccg gcgcgccaat tcgtagccgt ccatgtcggg
    13861 catgccgatg tcgactagca gcacggccgg ttgatacgtt ggcagtacct ccaaagctgc
    13921 aaagccattg tagacggctc gagcctcggc tcctgccaaa ttgagcagtt taccgagact
    13981 atccgcggca tcacggttgt catccaccac taacacccgc ttggaaggta gctgcccggc
    14041 gtgctgttta ttgtctcgtg tttcggtgcg caggttatca tcaaggggta ggcgcacaat
    14101 aaattcgcta cccttgcccg gaccttggct tcgagcctcg atcgtacctc catgcatttc
    14161 gatcaggctg cgaaccagag tgaggccgat acctaacccg ccctgggtgc ggctggtcga
    14221 gtgctcgagt tgcgtgaaca tttcaaagat acggggcaac atgtcgggcg agatgccgat
    14281 gccactatct cggaccgaga tcgatagatg atcgccctcg cgctgggcac gcagccagat
    14341 tcgccctccg ggattggtgt acttggcggc attattgagg agatttgaaa cgatctgtgc
    14401 cagccgtacg ggatcggctt cgaagatgag cggcgcggtc ggcaatgaga tctcgagatg
    14461 atggcgggct tcctcgatca gcggcttact cacttccacc gcgctctgga tgatagttgt
    14521 caataacacc cgttctttgc gcagttcaat ttggccgcga gtgatccggg atatctccag
    14581 gagatcatcg accaagcgca ccatttgatt gacctgacgt tccatcattt cataagtccg
    14641 ttcggtgccc ggcttcgaat cgccggcaag ccggaggatg tgcaggctgt ttcgaatagg
    14701 ggccagcgga ttacgcagct cgtgcgccag cgtggcgaga aattcatcct tgcgccggtc
    14761 ggcctcgcgc attgcttcct gagacttgat gagttcggtg atatcggcgg aacttccgac
    14821 atagccggtc acttcgcctg atggggcgag gcgtggattg ccgcgcgatt caatccagcg
    14881 ccattggtta tcgtgacgtt tgactcgtgc ccgcgcatgc aacggctgtc gatcgcgcag
    14941 cgcaacattg aatgcgtcca agtactctcg atcgtcggaa tgaataatct cggaccattc
    15001 gagccgggcg gctctttcac gggtagtgcc gaagaaatcc agataggagc gattgacgaa
    15061 ctcgatgcgg ccagaggcat cggttaccca aatcatcacc ggcgaactgt cagcgatgga
    15121 gcgaaaacgt tcctcgcttt cgcgcagctt ttcttccgcc aacttgcttt cggtaacgtc
    15181 aatgatgaat tcaacaccct catctgcctc gattcgtgtg gcggcgaaca aagcccacca
    15241 gcgcgatcca tctttgcgga tgtattcttt ctcgtaggga accgtgcggc ccatcctttc
    15301 gaactcctcg atcccgcgca gcgatcgagg catccactcc ggcggagtca ttctgtccca
    15361 acgcaccagc ccggattcca gatcgctacg gctgtagccg ctcatacgca ggaaggcatc
    15421 gttggcctcg atgatgcggc cgtcggtctt aaaaaatatc acacccaccg tttcaatctg
    15481 tatcgcgcga cccaatcgct cctgcgacgc ttgtagcgcc tgctcagccc gcttgcgatc
    15541 ctcaatcttt tggacaatgc cgacgaaccg cgccgggttg ccgtaagcgt cgcgcaccac
    15601 ggttccgtta caatgaaccc agacataccc gccatccgca gattcggcac ggcactcggc
    15661 cgagaacgtt ccgccttgtt caaatgccgg cagcgaagcc tggatgaccc gctcgcgatc
    15721 ctccgggtga atcacgcccc tcgcgcgctc taccgaaaag tatttctccg gcaatgtctc
    15781 gtagccgaac agttgcgcca tgttctccga gtactttatc gtaccggtca atagatcgac
    15841 gtcccacgca aacacgccgg cagcctgcgt tgccagtcgc atgcgctcct cggcttcgta
    15901 caattttcgt tcagtttcct tccgcgccga aacattgcgg aaaatggcgg tggcccgcgg
    15961 cgcttggccc gccgcgcgcc gcagcaggct tacgttgagg tgattccaga aagtcgagcc
    16021 atgtttgcgc agcaatctgg tttcaataga gatcaccagg atttcaccgc gcgaaagctg
    16081 ctgcagtaag agcgcggtgg cgtcgcgatc atccggatgc gtcagctcga cgatcgtccg
    16141 cagagtcaac tcttcggggg agtagccagt catctcgcac attttccgat tgacccgaag
    16201 gaatcgccca ctggcagggt ggatctgaca ttgaccgacg ctcgaaagct caaacattgc
    16261 acggaattcc gcttcgcttt gaggcaagtt ctcttcggtg tgattcgaag cggcggcttc
    16321 gacagtgccg atcatcgcgt atgccgcgga tgcgaaacgg gaaaggctgg tcagcagccg
    16381 cgcgtcctcc gcatcgaact tccgttccgg actatgagtt agaacccaca acgtgcccgc
    16441 cggttcgcca ccgctgtgaa atggcaccga gagattttcg aagatcggca ggttcatttc
    16501 ccggaaagcg acgaaatgtc gttcagggcg atcgaaaaga acgacagagt cccgctcgag
    16561 cacggttccg cacgggcacg catcgcggac catcgaagta ttgacgttcg gcgcataatg
    16621 tccggctatg gcacgccagc ggaacactcc ggcgccttcg tgtgtttcca ggatgcttat
    16681 tccggcggaa tcggcgtgac acagctcgag cgcgatatct gccaactttt gcaagaaagt
    16741 tcgcggcgcc tctttcatgg cctgggccaa cgcgatcagc gctcggttct cggctgcata
    16801 ttcaggcgca cgcgagctac gccggaaaag ctccgaggtg ttcagtatct ggttgacaga
    16861 taaccgctcc tcgtttgact ctgcacttcg atgatcgccc atcgctatgg cctttcggga
    16921 ctcgtttgta ctcctacgac cgacgcttag atggttgtgc agcagcgttt aaggatatgc
    16981 tggcggttag aagcgttctc gacatcgatt aagcttccaa agtcatcggc aacatcagga
    17041 aagccccgcc gccgatcggt tggcggtcga tatcttcctc gacatcccca ggatgattga
    17101 ctgaatcgat ctacagcggg aacttatttc ttataacctg cccaatgggt tgtaaagatg
    17161 tcgttgaacc tggatagagc ggcatcgata acgaccttgt tccttgttac gactcagctc
    17221 tcagtagcaa tggtactaaa tggcttatct cgaatccatg cgaactagcg ctttttatag
    17281 agcccgtcaa tgaagccgtt atcatcgtac tccttgatca gactcatatc gacgaagtct
    17341 tccggccgcg cggcgcgcgc tttggcgtct ttctcgccga gctcggcgag agcttctttg
    17401 acggaatcga gcgagggata cggcttctcc ggcaccaggc gagagaagaa ttggtagctg
    17461 tcctccagcg cgtcgttatc gctggtcttg acatattttc cgatcacttt tttgctaaac
    17521 tctttgtcgg ttttgtaaag gtggatgcct tcgagcaagg ctcgggtgaa tttgcgcgcg
    17581 gtgtcgcgct tctcacgaag gtaggagact cgcgatgcca caggggtggt cggataagcg
    17641 ccgccaaaat cctcaggttg agcgatgatg ttcatattga atttacgagc catattggtc
    17701 acggggggtt gggcgaccat gccgtgcacc tgctttgttt gaatcgccgc gagccgggag
    17761 ggctggccgc ccgggatctg caagatcgcc acgtcaatgt cgggcttcag gccccatttg
    17821 agcagtattt tcctggttgc ataatccgag ttggaaccga aacggctgac cgtgagtttt
    17881 ttgcctctta aatcttccgg ccgtttgatc tccggcgaaa ccaccatgct gtaggcgaga
    17941 acgttcagca ggccgctgat cagggtcacg tcagctcctc gaagcttggc gacaacgggt
    18001 gcgttgccgc cgacctgagc gatgggaaga tcgccggaca tcatcgcttg ggtcgctctg
    18061 gagccgccgt cgaggaagat catgaccacg tcaaggccgt tcttttcgaa cagtccggcc
    18121 tccttggtaa tccaaaatgg cgcctgcgag ccggcaaccg cgctccagcc gatcgtgagc
    18181 ttttctaaag gcgcggacgc cgctaaagaa atttcaaatt gcgacggcaa cgaaatcagc
    18241 atcgtcaaac tggccgcaat gattcttgtg cgtttcatat tggtctcctg ccttctcagt
    18301 gctgagagtg cgtgcatgca atcacgggga atcgaatatc cggggttgat cgatggagtt
    18361 gaacccaagt tagatttctc gcgccctcat acgccaatgc tcctgccggc gattcatctt
    18421 tctagaacgt agacccgttg aactttgaac gttggtgagc gctaggccgc actgacccac
    18481 ttattcacat cgacctccca gcctttggtc tgcgacaaat ccagcgcgtt accttccgga
    18541 tcgcgaaatc ggacttcggc gtagggtcgg gtcggtgggc gaggcgcgat gtttagcacg
    18601 ttgcgcaatt cggctaaggt cgcctccatc gtattggtga gaaatccgat atgattgatg
    18661 ccgaatttcg cctcatggcc ttcgatgggg ttgtagaacg ggtgtatagc gagattggtc
    18721 tggccgtcgc cgcagaagcg attgccgcgg ccttgctgcc ggcgctggta gctggacggc
    18781 acctcgcgca agccgaacac ttgagtgtaa aagcgcagca tcatttccgg atccaaagcg
    18841 ttgtaggcga tgtggcgaat ccgcgggaac ttcttttctt tctcgggcgg cacgccgaac
    18901 cgtcgggtcg acaagctcac gggaaaacat tccgggtcgt gaattctgat ctcaccgtgg
    18961 tgaatatcgt ccgcttcctg gaggatcatg ccgcgcggat tcagagctaa aaaacgtcct
    19021 ttggtttctt ccagatcgtc gaccgccaaa ccgatatgat gcagcccgca ctccattttc
    19081 atctcgttta attgagcgcg tcttttgaaa aacgtcagat tgtagaagcc atcggtgatc
    19141 gagatgtcgc cttcggctga gcggccgagt tcttcggcgc caagaaagcg acgatagaag
    19201 tcgcttactt tttccggatc atcactgagg taagcaatgt aacgtaagag cgccatccga
    19261 gcctcttttt tgatttaaat tcaaacaggc aatgctccat cgctgcgtat tctagtcacg
    19321 aatattctgc gtgaaccatt ccagcggcag cggatgtccc aggcctttag cgcgcgccag
    19381 attgtaagcc gtgccgccga cggccgcgaa ctgcaggcct tgcgtgccct gattgtttag
    19441 aaacgtgatc tgttgatcat tggcgcggcc gatccaacgg ccggccatga catcgggcac
    19501 ggtgccgatc tccggattat cgatttcgta atgcggcgcg gagggaatct tgttcttttc
    19561 gtcaatattg ccggcgatgt aaccgaacat gccgtcactg ccggtggtta cttccggagc
    19621 gttgcggtcg gcgaacagag tggatgtgcc aagacgggct attacgtcgg cccgctttag
    19681 aacttcaggg ccggcttcgt tattgcgcac gttattgata tgcattccgg gctcgagcca
    19741 ttcggccttg atcaccggaa ccagcgagtc ggtggtcagc gcaaccacgt cggcgcctcg
    19801 gagcgctttt tccggcgagt cgaccgcgat cacttccatg tccagcttct cggtcatctc
    19861 agcggcgtaa gcttcccggt tgactctggt cgggctgaac acgcgcatga cctcgatggg
    19921 tctgacttgt ttgatcgctt cggcataggt gcgcgccatg ccgccggagc cgatcatcgc
    19981 caggactttc gcgttctgcc gcgccaggta cttggttccg agcccggcgc aggcgccgac
    20041 ccgcatatgc tgcaggtagc cgtcgttgat gattgccaac ggctcgccgt tgcgtgtgct
    20101 ggtgagaaat atcagaccgc aataggttcc gcgctcgatg cagtatttat cttcgacgat
    20161 gaagccgtct ttgttttccc acgccagcat gtctgatttc atgcggatgg cgaagatgcc
    20221 aagcttgcgc gatgcgcctt ccatggttcc ccatcgatag tagtgcggct cttgcggaac
    20281 atagaaatcg attcgcggcc ggtagcccgc gtgcagttga ttgagctcgt gataagcctc
    20341 ctccatcgct tgaatgcagg tgggcatgtc caagacctgc tcaacgtgag cattggtaag
    20401 aaaaatcatg atctctccta ttgttttcct gattccgagc gaatcgatcc gctttgctcg
    20461 aggcgctcaa gaatcgaagt gtcgacgaca tcttgcggtt tcaactgggc gatcttggga
    20521 ttgcgcgaac ccatgatctc gatcgtcgct ttgacggcat ccatattcgg gtaaggcctg
    20581 gcttcgacct tgggtagcaa gtcttcatac gtccgtctgg cttcctcgcc gcttagccgg
    20641 agatgcttgg ccaggatctc ggtgactctc gcccggttgg ccggattcag catgaaggcg
    20701 ttgccttcga caatcgatcg aacgacagcc tcggccgtgc gaaccttgct tttcaggaat
    20761 gaacgagtgg aaaccaatgc gttgttgacg aatggaactc ccaagggctc gaggtttccg
    20821 agataggtga aaccgcggtc tttgatctgc agcgacacgg acggatctat aatggtcgcg
    20881 tcgattcttc cggcgatcag cgcttgcgcc cgggtaggct catcgccgat ggctaaaaaa
    20941 gtgatgcggt cgcgtttctc atcaagtttg aagtgttgca acatgagaaa cgatgcgaca
    21001 tgcgatgagc tgccaaagct gctgatcgcg agagacttgc ctttcaagtc ctccggtttt
    21061 ttgatttcgg gctttgccca aatgtcgtaa gtcaatttat tattgatgcc aaggatcatc
    21121 acggtttcgg cgccgccggc aacggctgtg accaccgggg ttgcaccggt aaagcccagc
    21181 ggcgcggaac cagacagaag cgcttgagtc atgcgttgac cgccggtaat cagcacgatg
    21241 ttggcatcta agccgttctt gcggaagatg ccgagttctt tgggcaccca gagcgcggcg
    21301 atatcgttgc gcaggttgga gtggccgata gcgagcttct caagttcttg cgccgtgacc
    21361 ggcgatgacg acgagcagcg ggcgatggcc aagatcaaga taacaacttc gcgatatcgc
    21421 ttcatctaaa taaaggatcc ttatccgtcg aacgatctag acctacttat gtgagccggc
    21481 tgtataaagt caacaaacgg attcggtggg atgccttgat tcaacagtca aagcggcagg
    21541 agccgggagg aacgagcacc tggttcagtt taagtttatt ccatgtagat ttctcgcatg
    21601 ttcggaatga catgagaaca cgtgatctgt catctcaacg aagtgagaga atcttcccaa
    21661 atgaaccgct atcaggagcg atgttatctt gacagtgaaa tctgtccatg gtcaacctga
    21721 aggggtggag attcgataaa ggaactctat gaaaacgatt ttaaccgtcg tgctcgcatt
    21781 atgttggttg gcgttgccgc cgtgttctgc cgcccaggag aaattccgcg ctgcgagcgg
    21841 cggtttttcg accgcaatcc atgcgctcct gtggctcgcg tacgagaaaa agatttttca
    21901 gaaatacggc ctggacggcg agtacctggc gctggagagc ggcacgccgg cgatgcaggc
    21961 gctgctcgcc aacgaactgc aaattgtctt taccaccggc gctctggcga tcaccgccaa
    22021 tttacaggga gccgatgcga cgattattgc cggcggcatc aacttcattc ccaataagct
    22081 gctggttcgt ccggacatca aaacgccgga aggtttgaaa ggaaaacgaa tcgccatcag
    22141 ccgcttcggt tcggcatcgg actacgcaac tcaagtggcg ctggaaaagc tcggcattaa
    22201 ccccaaagac gtgaccatcg tccaggtcgg cggcaatatg acgcggctcg ccgctttgag
    22261 ccagggcacg gtgcatgcga ccttgttaag cgagccgcac accaccgtcg gcatgaggga
    22321 cttcaagcta aattcgctca tcgatttggc cgagagcggc gtgcccttcc cgcagaattg
    22381 cttcatcgta aagcgcagct acttcgaggc caatcgcccg aagatcgtca atgccatgaa
    22441 agcgatcatc gagagctatt tcattttgcg caaggacaaa ccgctggctg tgtctttgat
    22501 gaagaaatac aaccgggtca gcgatgagga tgccagtctc ggctatgact actacctggc
    22561 caagtatggc gacggcgtga tggtgttgcc cgaccgcaag ggattggaat ttataatttc
    22621 gcaggtggcc gccaccaacg ccaaagccaa ggggcagaca ccggagagct tgaggctcct
    22681 ggatggcagc gtcttggaag aaatcaaaaa aagcggattc atcgagaaat ttaagaaata
    22741 gcccagtggc tcggataatc atccgaagat gttgaggtac gaagcggggt gatcattctt
    22801 acgattgaac ggcttgaact acttgaacag tttcaacgaa ttctttgatc ggagcgaagc
    22861 catggctggt gtgatcgacg cagatacgca tatcgccgag tccgaggcaa tgtggaaatt
    22921 tatcgatcca gagatgtatc cacgccggcc tatcctggcg aaaattcccg acgatacctg
    22981 gtacaaagat cgaaatgcgt tttggctgat cgatggcgag atctttccga aacctgcggg
    23041 taaggctagc tttagtttga tcacgccctc ggccgccaag agagaagccg gacggggaga
    23101 cattcacctg gcggtgcgag aattgaccga tcccatcgcg cgtctgaagg acatggacag
    23161 gatcgctgtg gaagtacagg tgatctatcc gacacttttt ctggtgtatc tcaccgacga
    23221 tcctcagtta gagacagctc tgtgccgcgc ctacaatcgc tttgtcgccg atgcttgcgc
    23281 taaagcgccg gaacggctga agtgggtggc gatcctgcca ttacgggcgg tcgatgcggc
    23341 gttgaaagaa ataacttggg cgaagcagca tggggcggtc ggtgtattct ttcgcggcat
    23401 ggaagggaat cttacgctcg acaacccata tttctttcct gtttacggcc aggcggagca
    23461 gctcgatctg ccgatctgta tccataccgg ctccggcagc cgctatctga tgcagctctt
    23521 cgacttagag cgcaaccata cttttgcgca taatcgggtg ctgccggttt ttgccttccg
    23581 cgatctcgtg cacaatcgaa ttccggaaaa gttcccgcgg ctgcgcttcg gttttatcga
    23641 agccgccgcc agttgggtac cctacatctg gcatgtgctg cggcggcaac agagcaactg
    23701 gagattcagc gatccgcagg agctctttcg cgagtaccgc ttctgggtcg cctgtgaggc
    23761 cgatgaagag ttgccgcacc tggttcggtt tctcggcgag gataagttaa tcatcgggtc
    23821 cgattacggc cataacgatc cgtccaagga gccgcagttt gtcgcgaatc tacgcgctcg
    23881 tgaggatgtg cccggcgcct tggtcgagaa gatcctgtgc gaaaacccgc ggatgttgta
    23941 tggcttgtca taaagtcgaa tcttcgccgc gcgggctgag agattatccg tattacgcaa
    24001 gccgttggct cttaggatcg tggccttaat ttcacggttt ttgcgaatat gcttgatgat
    24061 ccacgggaaa ttctgtattt gaagcgcatc gattatcaat gactcgcgcc ttgggcatgg
    24121 cgccaaatgt aaatgacaaa tcctagtcga gagtacacga tcctgaatgt cgacgatcac
    24181 gatgccgggc gctacgccaa gagccgcatt cttcaattgg cagggtaccg ggtcgttgaa
    24241 gcctctaccg gcgcggatgc cttgcgcctg gtgcgagagg caaaaccaca actgattttg
    24301 ctcgatgtca aattgccgga cgtaaatggc atcaaactat gccgcacgat caaaagcgat
    24361 ccgctgagca cccatattat ggtcctgcag atctcggcgg tgcacacgac ccgggctgac
    24421 cggatttatg ggttggaatg tggcgcagac acttatctga ttgaaccggt tcaggcggac
    24481 gagctcttgg caacggttaa tgcgctattg cgtctctatg atcgagagcg ggaaaatcgt
    24541 cagctggtgg cacagttgcg caatgcggat cgtcagaagg atgattttct cgccgcgctg
    24601 gcgcacgaac tgcgcaatcc tcttggggtt gtcagtaatg cgtttgatat tctcccggac
    24661 acgcaaacgc cggagccggt gtctcacgaa cttcgcgaca tcatcggccg gcagatcggc
    24721 ctcatgagcc gtttggttga tgacttactc gatgtctcac gtatctctcg cggccagatc
    24781 ggactcgcct gcgagccgtg cgacttcgct gctatcgtgc ggcaaacggt ggacgactac
    24841 cgagcgattt tagagtcaag cggtctggag ctaacactgg acgtgccgag ccacccggta
    24901 tgggtcgtag gtgattgcac tcgactggcg caatcgatca gcaatctcct gcaaaatgcc
    24961 ggcaagttta ccaaggccgg ggatgtggtg agcgtaaagc tagtcgacat tccagatgag
    25021 cactgcgcgg ttttgacgat tcgtgatacc gggattggta tggaatcaga tttcctggcg
    25081 cgcatttttg aaccgtttag ccagtttgat ccaggcatcg gacgcaggca aggcggcctc
    25141 ggcctcggtt tatcgctggt caaagggttg atcgaactcc acggcggaga agtgcatgcc
    25201 agcagtgaag gacttgggca cggttcagag atcactatcc gcttgccaac ccggatgttt
    25261 gccgaaagcc ctgacgcagc tcccaaaacg accatagccg atgctgatgc gccggcgagt
    25321 tatcgtattc tggtcatcga cgacaactcg ctggcagctc gtacgctgca aatttttctt
    25381 gccgacaaag ggcacgaggt cgaacttgcg gtcacaggtc cgcagggcct ggaaaaggcg
    25441 cggaagtttt tgccgcaggt cgttctctgt gacatcggcc tgccgggact ggacggctac
    25501 tcagtagccc ggcagctccg gcaggaacag ggctctgata aaatcttctt gatcgcggtc
    25561 tcgggttacg gccaagagga agatcaggaa cgcgccaaga gcgcgggctt cgacgcctat
    25621 ctggtcaaac cgattagctt gaaggatctt gagacactac tcgcggagct taatcattag
    25681 tggctgcgtt gtgcatgctc ttaggcaact ttggcccgag accattgggc gtcatcgccg
    25741 catcaaacat aaatgatccg cagtaacccc atgcgtctgc tgcagctgat tcaagacttc
    25801 gcacacccaa gccgcgcctg atccggcctg gcagtttttt cagcgccggc tgccgctccc
    25861 ctgttcatgc cgatccaagc cgaaccatga tcgagagcga tccaagattc gccgctgcgg
    25921 cttcaagctt attgcacgat tgctagaaaa gttggattta tggaggcgtt ttatcgtcaa
    25981 ggtgaacgcg gtgtttagaa attcggaggt tgattgtgct cgaacctgtt attacatgcc
    26041 cgcagtgcaa tatcgaaatt aagctcaccg agtcgctagc ggcgccgctg attcaatcga
    26101 ctcgccgtga atttgaaatg cggcttgcgc aaaaggatgc cgatgttgcc aaaaaagaag
    26161 cgatgctgcg cgatcgcgaa gcggcgatcg tgcgggccaa agcgcaaatc gatgaagaac
    26221 tcgctgctaa attgcttcag gaacggggca agattgccgc cgaagaagcg cacaaggcga
    26281 aactgtcatt agccgcagat ctggaacaaa aagccaaagc gattgccgaa ctgcaagaag
    26341 ttgtgaagga acgggagatc aagctcggcg aagcgcaaaa agctcaagcg gaagtgctga
    26401 gaaagcagcg tgagctcgat gatctgaaac gggaagtgga gctgactatt gaaaaacgag
    26461 tccaggagtc actcaccgca acccgcgagc aggctcgcaa agagactgaa gaagcgctga
    26521 gattcaaagt cctcgagaag gagcaaacca tcagctcgat gcagcgccag atcgaagatc
    26581 tcaagcgccg cgcagagcag ggctcccagc agttgcaagg tgaagtgcaa gagatcgagc
    26641 tggagaattt gttaagaacg aaattcccac gagattcgat ccagccggtt cccaaaggtg
    26701 aattcggcgg cgacgtcctg catcgggtca acggctctct cggccagtcc tgcggattca
    26761 tactctggga atgcaaacga accaagaatt ggagcgacgc gtggctcgct aaactgcgtg
    26821 aagatcagcg aaccgccaag gccgagatcg cggtgattgt cagccaggca ttacccaagg
    26881 acattgaaac ctttgaactg gtcgatggcg tatgggtgac gcatccgcgc gcagcgatgc
    26941 cggtggctgc cgttttgcgc cagactcttc tcgaggtcgc cgcggttcgc cagtcttccg
    27001 aaggacaaca aaccaaaatg gaaatggtct atcaatattt gaccgggcag cggtttcgac
    27061 agcgggtgca agcgattgtc gaagcgtttt catgcatgcg ggaggatctc gatcgagaga
    27121 agcgggcgat tatcaagcaa tgggccaaac gagaggagca aatcgatcgt gtcatgcaag
    27181 ccaccgtggg catgtatggc gatctccaag ggattgccgg taagaccttg caggaaatcg
    27241 agggactgga atttcctgcg ctcgatcaac ccaatatcga acagggtgat ctctgggggc
    27301 gggacaagat ttaacccgcg gttttcctca gccgtttgga tgatcgtttg gtccgccgcg
    27361 gcgccggcac agcaagaatc gggcaaggcg cccggcgcaa aactctttca gtgacgctgc
    27421 cgatcaacat atgggcaagc ccggtgcgac catgagtgga catgacgatg agatcgacac
    27481 tttcggccgc cgccttgtcg acgatccgcc tatacggtgt gccgatttcc acttcctgcc
    27541 ggactgtggt ggttccgatt aattcggtaa attcttttga gatgaaatca tccaacatct
    27601 ttttccgctg ctccaaagtt ttttttaact tcggcgactc ggtctgcttg gcaacccact
    27661 cttcagtgcc ttgaggaaag ggagtttcct cgactgtaag gacgttgtaa atcaagacct
    27721 ccgactgctg ccattgcgcc aggtcgagag cgaaacgaat gccggaaatg cacagactgg
    27781 acatatccgc cggagccagg atttttttga ttttcatcat atcgttttga gctcttctct
    27841 ctgacaatct tgtaccaacc gcgccatggg cagcggccgc acgctgaatc cctagatttt
    27901 ttcccatttc gacacgagcc attgtccccc gatcatggca atgacgaagc ctgccaggct
    27961 cggtgaagcc gaagccagtc cggccaatgc agggccggga cataggccgg cgagtcccca
    28021 accgataccg aaaattaccg ctccaactat cagcggtgtg tccatttcag ttttcgtcgg
    28081 cagtactaac cggtctccca ggaacgctcg gttgcgccga agaactaatg gaaacaggcc
    28141 agccgctacc agcagagcgc ctcccatgac cgccactaga gttggatccc atggaccggt
    28201 cacgtccaga aagccgatca cccgcgcagg gttgatcatt tcggatatcg ctagtccgaa
    28261 gccaaagatc gatccaatca aaaacgagct gatgctggct ttcatgaaaa gatatgtcgg
    28321 gtcaaataaa cggtcagtat agccactgcc atgaaagtca acgtcgccag cagcgagcga
    28381 ggtgaacgcc gagccaaacc gcagacgccg tgccctgagg tgcaaccgga tccgaggcgc
    28441 gtgccgatgc cgacgagcaa cccggcgatc gccagcacag gcccgtgaac ttccagttgc
    28501 aacggcaccc tacctcgcca gccgctatat agagcagcgc cggcgatcaa accaaaaaga
    28561 aacgcggcgc gccaggcacg atcatctttc actgttggga ccagcaaacc gccgagaatg
    28621 ccggatacac cagcaattcg cccgttgata gcaagtagca ggacggcggc caggccgatc
    28681 aacgagccgc cgatcagtcc tccaaccaga ttcggttccg tcatcaattg cttctgatgc
    28741 cgttatttcg ccggctcact tttgatttgc gccttggctt cttcgatcag aggttgaacg
    28801 gtctttttta aagcgggatc gagctgaaat gccttttgga aatcagcgtc cgcttcgttg
    28861 tctttttttt gatagacgcg ggctatgccg cgataaacgt gagcttcggc attgcgcggt
    28921 tgcaggcgaa tcacttcggt gaaatctctc gccgcgtagt cgtatttctc caagcgcaaa
    28981 taggcgatgc cgcgattgaa gggggcttcc ggatctttcg cattgagacg aatcgcttcg
    29041 ctgtagtctt ccacggccgc ctgatagttt ccaaccttcg ccgccgccag cccgcgatta
    29101 taataagcat cgctgaactt cgggctgagt ttgatcactt cggtaaaatc agctatggcc
    29161 gcgtcgattt tgtccgaatc cgagtaggca atgccgcggt tgtaaaaagc atcgaccaac
    29221 ttttcttcca acgagagcgc ctgggtataa tccttgatcg cctcgtccgt cttgttgaga
    29281 gccagataac tattaccgcg gttgtaataa gaatcggcat cctcagggct gaggcggatg
    29341 gcggcggaga aatcctccac ggcggcgtcg taacggcgcg cgtcaaaatg aatgctgccg
    29401 cggttgatgt aagctctgct atcctgcggg ttcagtttga tggcggcgct gtaatcgtcg
    29461 agcgcggcgc gctcttgctt cagccgccga taggcattgc cgcggttgaa gtaagcctcg
    29521 ctgtccttgg gcgccagact aatcgcttcg ctgtaatcct ttaccgcgcg ctgattctgt
    29581 cccaggtcgt agtaagcgtt gccgcgattg aaataacctt cgacccgctt tggataagtt
    29641 ttgatgaatc tgtcatagag gactatcgct tcttttattt ttcccgcctc gtgcagctgg
    29701 agcgcgtctt tgagcggctc gatcacctga gcgggccagg ccggttcaga gataaacccg
    29761 gccaacaata agacaagagc tttgattccg gcacgatgca taacgattac tgcctcttga
    29821 ctgcgggagc ggctttttga tcgctggtga tcgattggat ttgttctttc aagctcggct
    29881 ccaactccat cgccttgttg aagtcggttt gagcatcctc gcccttgccc tggcgcagct
    29941 tgaccatgcc gcggttcgca tagatccgcg cgtttttagg atcgagcttg atcgcttgat
    30001 cgtagtctcg gagcgcatga ttgaactggc ccagcagaga ataggcgttg gcgcgattgt
    30061 tataggcctc ggcatttttg ggttccagct tgatggcttc cgaataatcc tgcaccgcca
    30121 ggtcatgctt gccgagcctc cggtaggcga gcccacgccg ataatagaga tcggggtcct
    30181 tgggatcgag tttctgtgat tgactgaaat ccttgacggc attttcgtat tgtcccagcc
    30241 ctgagtaggc ttgccctctc tgatcatagg ctttcggcat ctttggattc tgtttgatga
    30301 cttggtcgaa gtcttttatg gcgcggtcgt attgtttctg cgtcaatagg gcgttaccgc
    30361 ggttgtatag tgcgtcggta aactgaggat cgagtttaac tgcctggtca taatccttga
    30421 tggcattgcc gatctgtcca agcttgtggt aggccaggcc gcggttattg taggcctcgc
    30481 tggcatccgg ggatgccttg atagcgcgat cgtaagccgc gatggcttct ttatacctgc
    30541 ccgcctgatg gtgtttaacg ccttgatcaa atgcattctg cgcttcgccg cccgcggcta
    30601 tgttgccgaa gggcaatgcc agggcgacaa acaagaaaaa aattctaaat actggcaaca
    30661 tcaaggtcga atttagcacc gaacccggaa ttgaacaatt tcacggccga tctttttata
    30721 tatttaagaa ttcacgcaaa gatcaccggg tcatcgctca tccctggaac ttcagcctat
    30781 ggacaaagca gtttggatga aataaaagta ggcaataaat caaaaaagcg agggcaaacc
    30841 ttatgagcat agccgcggcg cggacagcgc aatctgtact tgccgatgaa cggatcgtct
    30901 cggcggattc acacatcatg gagcctgaaa acctgtggga aaaaaatctt tccccatctc
    30961 tgaaaaccaa attccccaaa tttccccctc gtaacactgc gggagagaaa cccggcggat
    31021 ggaatcccaa agcccgcctc gacgaaatgg cagtcgacgg cgtcagcgcc gaagttcttt
    31081 acccgactca tgggcttcgg ttgttcgccc tggaagacgc cgaactgcag gaggcctgct
    31141 tcaagatcgc caatgactgg ctgatcgatt actgcgcggc ggcgccaggc cgcctggtcg
    31201 gcattccgat gatctcgctt tataatattc ccaacgcgat caaggaactg gagcgttgca
    31261 agaaagccgg gctggtcggc agcttgattt ggcaggtgcc gcctgagcat ctgccgttta
    31321 ccagtgacta ctatgatcct ttttgggaag cctcagaggc tttggagatg ccgcccaacc
    31381 tgcacatttt gaccgggttc aattacagcc ggttcgatcg caagggattg gacatctaca
    31441 aggctacggt caacaccaaa ctatatgatg ccgccaatac tctctttgac attgttttca
    31501 gcggagtttt cgcgcgtttt cccaagctga aaatcgttta cgtcgaaaac gaagtcggct
    31561 ggattccgtt cttcgttcat gaatgggaca agtatttcgt ccgtcatagc ccgaagatgc
    31621 cgttgcctta catgaaaaag ttgcccggcg agtatgttgc cgaacaggtt tatgcgacct
    31681 tctttagcga tccggcgggg ggccgtttga tggaagattt tggccaagat actttcatgt
    31741 ggtcgaatga ttatccccat gctgcctcca catggccgca ctcccgcgac gtaatcgcca
    31801 gggaattggg acatctaccg aaggagattc tacgaaaagt cgtgcgcgac aatgtgatca
    31861 agctgtataa tcttaaactc gacggcatca acaactaatc cggatatcaa cgagaaggag
    31921 aatcactatg caagcaagcg ccaccgcggc aaaatatgag aaggacgaaa agccgcaaac
    31981 caaactgttt tccctcagaa ctccgtacat gaagcaggga cgtatcaccc agctggtggc
    32041 ggaaaccgag aatatgtgga ttcacaccaa gatcaattat gaaggcggtg aaaatgagat
    32101 ccacacgcat ctcgacgagg accattcttt catcgtcctg gaaggacaga tgagcgtttt
    32161 cgatgaaaag ggcaatgaat tgaaagtcgg caagtaccag ggagtgatga tccccaaggg
    32221 cgcctactat cgttaccaca acaccggtcc ggacaacctg gtggtcatcc gcgttggcgc
    32281 cggcatcaaa ggaaagcctc aaggcggcga ggatatgcgc gtgcgtccgg acggcaagcc
    32341 gctgccggca tactcggccg agaacaaaac cgtcgagcct atcgaggata cgcgcttttt
    32401 cgcggatttc gcgggataac cgactggcac gcatacgcga ctaaaatgtt gctggacaat
    32461 ggccgctgat cgaatcagtg gccattgtca ttttggcagg cgagtcctcg tgatcgtgtc
    32521 cgtgcaaacg tgatcgagaa ctccgacgaa cacgaacacg cgagcacgaa cacaaagtgg
    32581 gtactttatg acgccgagca agacgacatt tacgtgggag acgccgcccg agctatggcg
    32641 cggtttaaag cccagcgctg cctggaaggg cagcgtggtg aacctttaca ttgctccaga
    32701 gcccgccggt ccgatgatct cggtatcgga ggtacgagct ctggccgatc gcggtttgga
    32761 aggggatcgt ttctttcgcg agtcttgggc agcagcgaag cgacccgaca aagctgtcag
    32821 cctcatcgaa caggaagttc tcgagatggc cgaatcggag cagcaactaa cgattgccgg
    32881 cgacaagacc aggcgcaaca ttatcacgcg cggcgtgccg ctgatcgaac tgctcgatcg
    32941 agagttcatg atcggcaacg tagtgatgcg cggtattcgc ctgttcgagc cctgcgctca
    33001 tttggagaag gtttcccgga tctcgggcat ctttcgcgcg ctggaaaacc gcagcggtct
    33061 aaaagcggcg attctcagcg acggcgtgct tcgtatcgga gatccaatcg cgctgcaggc
    33121 cgctaaaaaa gcctcataag ctctgttgta cgcgcaccgg ctcgcttcag ttttcgcgtg
    33181 ttcgtgttcg ttcggagttc tcgatcacgt ttgcacggac accacacgag attccgcctc
    33241 tggactggag cagcctgcaa ccaaggtttt ccagcaatcg ctagagctca aaccttcaaa
    33301 taggacaagc ctatgcagtt tggcctccat ctgccggcct caacggctgg ggtgacatct
    33361 caggacttgg taaggtttgc ccggcgcgcc gaggagctgg ggttttactg tattacggtt
    33421 gccgatcaca tcatcgtgcc gaagaatatt tcagtccctt atccctacac ggccgacggc
    33481 aaatacccgg gcgccggcta tcatctcgag acattgacca ccatcggctt tctcgccggg
    33541 gcgactaccc gcattcgttt cgtcaccagt gttatgatcg cgccttaccg taatccggtg
    33601 attaccgcca agatgctcgc ctccctcgat gttctgtccc acggcaggat cattgtcgga
    33661 ctaggggttg gctggatgaa ggaggaattt gagaatttaa agacgccgcc ataccgggag
    33721 cgagggcggg tgagcgatga atacatccac gcctttcggg agctatggac ccaggacgct
    33781 ccgtcgtttc aaggagaata ttgcagcttt tccgatatcg tcttcttgcc caagccggtg
    33841 cagaagccgg ccatccccat atggatcggc ggtcacagca ggcaagcttt gaaacgcgca
    33901 ggacaactgg gcgacggctg gcatccgatc ggcggagttc ccacgattcc gctcgaaccg
    33961 gaagatctaa agcaggatgt ggagattatg cgcgggtttg ctcaagccgc gggtcgagat
    34021 cccgacgcca ttcacgtggc cttcaaaggt tcattgtacg atcgcgaaaa acagatcgtt
    34081 cccggcaaga gacgaaggtt cgtcggcgcc gttgatgaca tcatcggcga cattcacgag
    34141 tatcgtgatg ccggtgtcga taccttgatc ttcgacgtgc gccgtcccag cgccgccggt
    34201 actctcgagc ggatggaatg gctggcgcag gaagtgattg gtaagataga gaaagcgtga
    34261 tcgtgttcgt gaaatgtgat tgtggtttgg gacgactagt cagtggtcaa gtctctcatg
    34321 gagtttttct gacgagcagg tccgaagatc cggtctcaag ccgcacgttt aggcagtact
    34381 ggatccggtg ttttcagcct gcatcggaag gggaccagac aatgcaccaa gccggcgcga
    34441 gatctccccc ggattctgcg aatctccaaa gttgataccg ccatagatct gaaaacgcca
    34501 cacggtgatc tgtggacaat cgacagcctg tgcaccttca tagcaaaagg ttccatgccc
    34561 tagatcgtcg ttcactctag ctgctacgtc cagactgaac aacttatcga agtacgtttc
    34621 tcccgcgcgc gcgagagcga gcacctgaac ctcatcttct tgcctgagat aggttccaaa
    34681 gtggaaacag acaagtcctc gcacaatgag agcaaagagc tgctgcagtt ttgtcggatc
    34741 gagaggcaac gtggaaaaac ggagatgaac acctccttct ttcgcccaaa ctctgcccaa
    34801 tcgtcccgca acgtgcgatg aagtttgaca ttctcggtaa gacgtttcgg caccatgttt
    34861 tgagatttgg cagtgccgct ttgtggcgac tccaaacggg aggaccgaag ttaaataatg
    34921 ctcgagcgcc gatttttgag cattacataa agagcatgcc ggaacctgtg gcagattcgc
    34981 cctggcactt ggcaagaaaa agttccttgc gaagacgtgg tcagcagttt ctgaaagcct
    35041 ttcgaagcag tatgcacatg gcttcccttt gtgcttctta cccatgtcaa atagctgtta
    35101 agggttggta atcccgattt gctgcttttt ctcgctactc aagaacgaaa ttccccttcg
    35161 accaaatcgc tgtcagctga agctgcggac attgcgatgc gttgacggaa agataattca
    35221 tatcgaccta actgaccggc tcttttagtg ccggcggctt cgatttacgc cgcggatcgt
    35281 cggcgtcggt caagcaaata tatgtcattg tcgtattgtt ctctgtgaat gtgacggtat
    35341 tttcgccgat gaccgcgtct ttgaaagctc gccatacggc caacttttct ttgatattgg
    35401 cggcgcactg ctcgcggctt tgaaaacccg gtaacgcatt ccagctatcg gccttggggc
    35461 tctgcgtgcg tatccataaa acccaggcga tcataaccgc taagtaacag aactttgtca
    35521 tgggctgctt cggctcctcc aaatcggcgg cgtagcggga agacagaata cccctaatgc
    35581 ttcgattggt acaccgttct cttgacacac aagtcggttg aggcgtagac ggtttgagag
    35641 gaacccaa
//
LOCUS       DASUVS010000239         5344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1192742, whole genome shotgun sequence.
ACCESSION   DASUVS010000239 DASUVS010000000
VERSION     DASUVS010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192742"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VF182_13125"
     CDS             complement(<1..263)
                     /locus_tag="VF182_13125"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6438073.1"
                     /translation="MLRVSRYYIAAHTAEGTLVGFARVCGDPYVAQVLDVITHPDYRR
                     RGIATRCMRGVLAHLQRSQYLSVTLTDGSGIAGFYQRFGFRVC"
     gene            796..1008
                     /locus_tag="VF182_13130"
     CDS             796..1008
                     /locus_tag="VF182_13130"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6438074.1"
                     /translation="MGDISAELQTKLSRFLQEREFERVGGTQSIAVDVRVIAATNRDL
                     LSAMKDGRFREDLYYRLNVIPVSLPR"
     gene            1005..1415
                     /locus_tag="VF182_13135"
     CDS             1005..1415
                     /locus_tag="VF182_13135"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6438075.1"
                     /translation="MRERQEDIPILAKYFIRRFATETKKTFTEITADAEAKLVCYGWP
                     GNVRELANVIERVVVLSDGPEIAGEDLPPRIADTETDGAADGFSYRRAVDSARADVIK
                     RALAHTHGNRAAVARSLGLHKTHLLNLMKTLGIE"
     gene            complement(1440..2315)
                     /locus_tag="VF182_13140"
     CDS             complement(1440..2315)
                     /locus_tag="VF182_13140"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6438076.1"
                     /translation="MIPGLREKSIDAAGLNIHYLEWGEPADDLIVLVHGFLDHAHSWR
                     PFVIALQGKSKKPLWIVAPDCRGHGDSGWVGAGGYYHFPDYVFDLHCLIESLGASRCG
                     LIGHSMGGSISFLYTGTFPQRVRSLVLIEGVGPPGSLFSDAPPRMEKWITEVRERGRD
                     HFRQYPSIKAGARQLQQTNPRLGSEFALELARTGMRQNASGKWVWKFDPLHRTTAPQP
                     FYMDQAIEFLRRIECPTLTIEGKESHQTKRTDKQRRFEAISNRRHVVIENAGHMVHQD
                     NPEALARAISDFLRL"
     gene            complement(2401..2874)
                     /locus_tag="VF182_13145"
     CDS             complement(2401..2874)
                     /locus_tag="VF182_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX6438077.1"
                     /translation="MNTRRRLVLSHESTKTICKHAEETFPEECCGIILSDGTTDYVQR
                     LNNIQNQLHALDPETYPRTAAIAYAMDPTELERIIDNALLNGAKLKAFYHSHPDHTAY
                     FSEEDKAFASPFGEPVFPETAQIVISIYHREIRDIRAYIWSDDEKDFTEVAIDNV"
     gene            complement(2948..3643)
                     /locus_tag="VF182_13150"
     CDS             complement(2948..3643)
                     /locus_tag="VF182_13150"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX6438078.1"
                     /translation="MEKRPKKTHTHTLRWAASCLRLALIVILLLPGLVASGYMPRHYQ
                     IIELTPPAEAQTIERPRSRDLVKIFSIIEANRPDIMEREAWELAEVISTESSKYRLDP
                     ILILAVIDVESKFQFGAISPAGARGIMQIMPATGKFLVKSVRELGREIKADQFTPEHL
                     DNPVVNIKLGTYYLHDLRKSFRNLNEALTAYNLGPTELRIRMENQIGYTDDYANAVLA
                     AHQRFKKIKPATF"
     gene            complement(3927..>5344)
                     /locus_tag="VF182_13155"
     CDS             complement(3927..>5344)
                     /locus_tag="VF182_13155"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6438079.1"
                     /translation="GCPILFNKVKGKPHHRCITNLFGDMNVIGKLFGWKDHADRTRKL
                     AYALTHPIAPVIIDQKDAPVQEEVYEKPAEANEFVVPIRHTDIEPELTVGSGNGLISG
                     EYFDGGSHIGYNRMNFRWGNVGTFQISPGSHSWQVVTKYYKDNKLVPITMNFGLPPSS
                     SLLAGSGFDYVILPQGCDEIGMAGAVQGFPVRLVKARTVDALAVADAEVVLEGYINPR
                     DRRFETAEAEKAGVQGRYHFHPEWAGYMGKAYKAPTFHVTAVTTRRRESKPIIFPLGV
                     HTLDDHNIDTTVREAAMFELCERMQPGIMMDVNIPYCMTDWGGAIIQVKKRNKIEEGW
                     QRNFMAAILATSQGSRLVIAVSEDTDPYDMDDVMWCLTTRVNPKTDIINPLPGGRGQT
                     FMPAERMTAGEREWTASNTMFEGGMGIDATVPFGYESDFMRPVYPVDKIDLKKWFSEK
                     DIQNAKARMRGWVHSLARTGR"
BASE COUNT         1225 a         1357 c         1423 g         1339 t
ORIGIN      
        1 ttgcagaccc gaaatccgaa tcgttgatag aacccggcaa tgccactgcc atcggtcaag
       61 gtcacggaca gatactggga tcgttgcagg tgcgccagca ccccacgcat acaccgggtt
      121 gcaatcccac gacggcgata gtcagggtgc gtgatcacat ctaacacttg ggccacgtac
      181 gggtcgccac agacacgcgc gaatcctacc aaggtgccct cggctgtatg ggcagcgatg
      241 tagtagcggc tgacgcgcaa catctcggtt gtttcggtct cggtgcgtcg gttatgacta
      301 tctcactagc ggcaacgggt ttagccgctt catagcagcc gactctgagg tccggtggga
      361 ttcattctcg acgcgagatt gtaactcagc caaatataga tcgcttgggg tgtatctcat
      421 cgcagcgccg tccaaagaat aacatcgtag agttttctct acgggaatag agaaaactct
      481 acccattgag tgtatggccg tctagcttag attccatcca ttgaaaatta ttccgttcac
      541 tcaacgtctt agtttcatcg agccccaagc cctgcccgtc gcacgccggt tgtgctaaga
      601 aaatcttcga ctctcatccc gctggttaag caaatttagc caagtctgat gcgacgtggc
      661 ttatgactac tgggttcgtc ttgcggatgt gtactcaaga agaagaaagg gagtgatttg
      721 cttgtttacg gctaaataga cagcgtcatt gacaggatcg agttggccca tggcggcacc
      781 gtttttctcg atgaggtggg cgatatatca gcagagctgc aaacaaaatt atcgagattt
      841 cttcaggaac gggagtttga gcgggttggg ggaactcagt caatcgccgt cgatgttcga
      901 gtgatagcgg caacgaatcg agacctgttg agcgctatga aagacggccg ctttcgcgag
      961 gatctgtact atcgactaaa cgttattcca gtctcgcttc cacgttgaga gaacggcagg
     1021 aggatatccc gatcttagca aaatatttta ttcggcgttt tgccacagag acgaagaaaa
     1081 catttaccga gattaccgct gacgctgaag ccaagctcgt ttgctacggc tggccgggga
     1141 acgtccgcga gctggcaaat gtcattgaac gtgttgtcgt actaagcgac ggaccggaaa
     1201 tagccggtga ggacttgcca ccgcggattg ccgacacgga gacggacggc gcggcggacg
     1261 ggttttcata tcgccgggcg gtggattctg ccagagccga tgttatcaaa agagcccttg
     1321 cacacaccca cggaaatcgc gccgccgtgg cgagaagcct ggggctgcac aaaactcatc
     1381 tcctgaatct aatgaaaacg cttggtatcg agtaaagatg agcgtcgatt gtggtgtgac
     1441 tacaaccgga gaaaatcgct tattgcccgc gctaatgctt caggattgtc ttgatgcacc
     1501 atatggcccg cattttcgat gacgacgtga cgccgatttg aaatagcttc gaaacgtctt
     1561 tgtttgtcgg tgcgtttggt ttgatggctc tctttgcctt cgatcgtaag cgtcggacac
     1621 tctatgcggc gcaggaactc aatagcttga tccatataga aaggctgtgg cgcggtggtg
     1681 cgatgcagcg ggtcgaattt ccaaacccat ttgccgctag cgttttgcct cattccggtg
     1741 cgggccaatt ctaaggcaaa ctcactacct aatcgcgggt tggtctgctg tagctgcctg
     1801 gctccggcct ttatgctggg gtactgccga aagtgatctc gacctctctc gcgcacttcg
     1861 gttatccact tttccatccg aggcggcgcg tctgaaaaaa gcgatcccgg agggcccacg
     1921 ccttcgatca gcaccaaact gcgtactcgt tgtggaaatg tgccggtgta gagaaacgat
     1981 atcgatccgc ccattgaatg accgataagt ccgcaacgtg acgccccgag actctcaatc
     2041 aagcagtgaa gatcaaaaac atagtcagga aagtgataat agccgccggc gccgacccag
     2101 ccgctatcgc cgtgccctcg gcagtccggt gcgacaatcc acaacggttt tttactcttg
     2161 ccttgaagag cgatcacgaa cggtcgccag ctgtgtgcat ggtctaaaaa accatgaacc
     2221 agaactatta aatcgtctgc gggttcgccc cattccagat aatgaatgtt caaaccggcg
     2281 gcatcgatac ttttctcgcg aagcccgggt atcaagctat tctcattcca ttacgttaca
     2341 tttcttggcc gaaaattgcg ttcgaaacag cagagctgat tcgttcagct actgatttgc
     2401 tcatacgttg tctatagcaa cttcggtaaa atctttttca tcgtccgacc agatataagc
     2461 acggatgtct ctaatctccc gatgatagat ggatattacg atctgggcgg tctcaggaaa
     2521 aacaggttca ccgaaagggc tggcaaaggc tttatcttct tcggagaagt aggcggtgtg
     2581 atcagggtga gagtgataga aagccttgag tttcgcgccg tttaacaaag cgttgtcgat
     2641 gatccgttcc aactcggttg gatccatggc gtaggcaatc gctgccgtgc gcgggtaagt
     2701 ttcgggatcg agagcgtgga gctgattctg aatattattc aggcgctgga cgtaatcggt
     2761 tgtgccgtcg ctaagaatga ttccgcagca ttcctcaggg aacgtttctt cggcatgttt
     2821 gcagatggtt ttcgttgatt cgtgtgagag cactaaccga cggcgtgtat tcatcttcgc
     2881 gcacctgacc ttcttttagc acaaggacgg caggcgttgc taatctaaca aacagatgga
     2941 ttaaatctca gaacgtggcc ggcttgatct ttttgaatct ctggtgggcc gcgagtacgg
     3001 catttgcgta atcgtcggtg tatccgatct ggttttccat acgaatccga agttcggtcg
     3061 ggcctagatt gtaggcggtt aatgcctcgt tcaagtttcg gaagcttttt cggagatcgt
     3121 gcaaatagta ggtgccaagc ttgatattga cgacgggatt atcgagatgc tcgggcgtga
     3181 attgatccgc tttgatttct ctgcccaatt cgcgcacgct ttttacaaga aatttcccgg
     3241 tggccggcat gatctgcata atgccccggg cgccggcggg agaaatagcg ccgaattgaa
     3301 acttactttc gacatcaatt acagccagga tcaaaatcgg atccagcctg tatttagaac
     3361 tttccgtcga gatgacttct gccagttccc aagcttcccg ttccatgatg tcaggccgat
     3421 tcgcctcaat gatggaaaaa attttgacga gatcccgtga gcgcggcctt tcgattgtct
     3481 gggcttccgc gggtggagtg agttcgatga tttggtaatg tcgcggcatg tatccgctgg
     3541 cgacaagtcc tggcagtaat aagatgacga tcagcgcgag cctcaagcag ctcgcggccc
     3601 atcgtaaagt atgagtgtgc gtttttttgg gacgtttctc catacttaac ctcagtggat
     3661 ttttgttggt ggtggatctg cgggagcgaa gatatagaag ctacgagtta ggtaacaaac
     3721 aagagaattt ttgtcaatgt aaatttttgc agctgttgca ttcacaagcc catccgtaca
     3781 atgccgggat cttgatcttc gaattttcga agactctcag ggtgagcaaa tgttccttac
     3841 attaaaaaca aagggcaggg attatccctg ccctttgtac tccgaaccta aactgcgtga
     3901 tcgaaaaaaa gcgtgctgct ggaaaactat ctacccgtgc gcgccaatga gtgtacccaa
     3961 ccccgcatgc gcgccttggc gttttgaata tctttttcgc tgaaccattt tttgagatct
     4021 attttgtcga caggataaac gggtcgcata aagtcgctct cataaccgaa aggaaccgtg
     4081 gcgtcgatgc ccatgccgcc ctcaaacatc gtattcgacg cggtccattc ccgctcgccg
     4141 gcagtcattc tctcggcggg catgaaggtc tggccgcgac cgcccggcag cggattgata
     4201 atgtcggtct tgggattgac ccgagttgtt aaacaccaca tcacgtcatc catgtcataa
     4261 ggatctgtgt cttcgctgac agcaatgacg agccgggatc cttgcgaggt cgcaagaatc
     4321 gccgccataa aattgcgttg ccagccttcc tcgatcttgt tgcgtttctt gacttgaatg
     4381 atagcccctc cccagtcggt catgcagtag ggaatgttga cgtccatcat gatgccgggc
     4441 tgcattcgtt cgcacagctc gaacatcgcc gcctcacgca cggtcgtgtc gatgttgtgg
     4501 tcatcgagcg tgtgcacacc gagcggaaat atgatcggct tgctttcacg cctgcgcgta
     4561 gtgaccgcgg tgacgtgaaa agtcggcgcc ttgtaggctt tacccatata gccggcccat
     4621 tccggatgaa aatggtagcg gccttgtacg ccggcctttt cggcttcggc ggtttcgaaa
     4681 cgccggtctc ttggattgat ataaccttcg agaacgacct cagcgtcggc aaccgccaac
     4741 gcatcgacgg tgcgcgcttt aaccagccgg accggaaaac cctgcacagc tccggccata
     4801 ccgatttcgt cgcagccctg cggcaggatc acataatcga aacctgagcc tgctagcagg
     4861 cttgacgatg gtggcaagcc aaagttcatc gtgatcggca caagtttgtt atctttgtag
     4921 tacttggtga cgacttgcca actatgtgat ccgggggaaa tttggaatgt accgacattt
     4981 ccccaccgga agttcatccg gttgtaacca atgtgtgatc cgccgtcgaa gtattccccg
     5041 gagatcaagc cgttgccgga accgacggtc agctcgggct cgatatccgt gtgacggatc
     5101 ggaacgacaa actcgttcgc ctcggctggt ttctcataga cttcttcttg gaccggagcg
     5161 tccttctgat cgatgatcac cggcgcaatc ggatgagtca gagcgtaggc cagtttcctg
     5221 gtgcgatcag cgtgatcttt ccagccgaac aacttgccga ttacgttcat gtcgccgaat
     5281 agatttgtga tgcagcgatg gtgcggtttg cccttgacct tgttgaaaag aatcggacac
     5341 ccac
//
LOCUS       DASUVS010000240        14561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1192887, whole genome shotgun sequence.
ACCESSION   DASUVS010000240 DASUVS010000000
VERSION     DASUVS010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14561
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192887"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1075)
                     /locus_tag="VF182_13160"
     CDS             complement(65..1075)
                     /locus_tag="VF182_13160"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438080.1"
                     /translation="MQLPPVATTTVGSFPRPAWLAKSERSQVKFHWQGTALKEAQDDA
                     TRLILQTQQQIGLDLLTDGEQRRIGFIHHILAAFDGIDVENQAVKKIYRRREQEHLVP
                     RIIGKVKRRGSVNVEDLRFARRQTSKPIKMAVPGPMTVIDSTLDETYNDEPALAMDIA
                     AAINSELLELQAFGCDVLQVDEPAMTRYHEKVFAYGARALDRCLQDIRIPTIVHLCYG
                     YPGGADRQHQYEYPELLEELMKTKIGGFAVEFARSGYDPAVLEICKGRKIMFGCVDPS
                     DAPVPPVETVVQRVRHALKYIDPANLLLAPDCGLMTISRELADVKAKLLVDVAKEVRK
                     SI"
     gene            1486..2652
                     /locus_tag="VF182_13165"
     CDS             1486..2652
                     /locus_tag="VF182_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438081.1"
                     /translation="MATTNELRLYLLTAEGLIRCGWDGRSEHVEILNSALAGEVLREV
                     AQDPFDRNHLYAATITEIHVSEDGGAHWKWLPSGGIDYRDIWAMDVDPTRRDQIYVGT
                     LPAAVYVSENGGRSFRELSAFRKLPEYGKWTFPPAPHSAHIRCFALDARFPDEIIVGI
                     EEGGIARSRDRGESWQDISGPQSAMAYPEYNDPAGIKPYQMGQHEDGRVYRDVHWVLR
                     HPRSVNRLYATTGIGTYRSDDDGKSWIKAEYGMGRAYAIPIAIHRDSPDRIFLGAAEN
                     GPTSWLGHRTVRAGPYNTIKFSPDTSEKLGGAKTGILRSDDAGESWRRLNNGLPQSYP
                     HMTCGFALHPQDGNTVCVSYTDGSIYASNDAGESWRKLDVTVSKLYGIRLMTAN"
     gene            complement(2708..3586)
                     /locus_tag="VF182_13170"
     CDS             complement(2708..3586)
                     /locus_tag="VF182_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HEX6438082.1"
                     /translation="MNSRQLLKQLFKRDRLLVAPGCFDGLSARLVEEAGFEAAYLSGG
                     AVARSMGIPDIGLVTMSEVIERAAQVVAAVKIPIIADADTGYGNAVNLVRSVREFERT
                     GVAAIHIEDQITPKRCGHLDGKEVIPLAEMENKLQAALSSRNDPDFLIIARTDARGVH
                     GLDDALRRGRAFAELGVDAVFVEAPQSEAELEDIPRALPGVPLLVNVFKGGKTPMLPV
                     ERLQQMGYRIAIYPSETQRAAIHAMRQALGLLKREGTTEKMDDALTTFKERDKVVGLD
                     EWQRLEKQYMALSEWS"
     gene            complement(3851..5290)
                     /locus_tag="VF182_13175"
     CDS             complement(3851..5290)
                     /locus_tag="VF182_13175"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HEX6438083.1"
                     /translation="MTSTTLNIRPSSDALLLEIAAYAADYDASSAEAIDTARYCFMDT
                     LGCGLLALRYSDCTNQLGPVVPGALLANGARVPGTAWQLDPVHAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILALADYLTRSGQRSFTVGNVLTAMIKAHEIQGVLALENSF
                     NRVGLDHVLLVRVATAAVATAMLGGSREQIVSALSNAWTDGGSLRLYRHAPNTGPRKS
                     WAAGDATSRGVRLALMAMRGEIGYPSALSAKEWGFYDVLFHGNPFELGRPFGSYVMEN
                     ILFKISYPAEFHAQTAVECALRLHREVAHRLEDIEKVRITTQESAIRIIDKTGPLYNP
                     ADRDHCLQYMTAIGLIFGDLTADHYQDAIAKDPRIDALRIKMEVVEDPRYSKDYLDPD
                     KRSIANAVQVFFTDGSASENIAVEYPVGHRRRRAEGMPLLIKKFETNLTSRLAPAWCD
                     KILALCLDLKTLHSTPVDVFTDMFTVESA"
     gene            complement(5598..6716)
                     /gene="prpC"
                     /locus_tag="VF182_13180"
     CDS             complement(5598..6716)
                     /gene="prpC"
                     /locus_tag="VF182_13180"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEX6438084.1"
                     /translation="MATDKTGGLAGIIAGNTSIATVGKEGKGLTYRGYSIYDLAEHST
                     FEEVAYLLIYGKLPSGSQLREYKENLRKQRGLSSPLKAVLEQIPGSTHPMDVLRTACS
                     MLGTLEPENKEHDQIAIANRLTACFSSMLLYWHQYVNTGKRIDTKTEDDSTAGHFLHL
                     LHGKKPDELCRRAVDVSLILYAEHEFNASTFTARTIASTLPDFYSAVTGAIGALRGPL
                     HGGANEAAMELLEKFSTPEEAESGILEMTGAKKLVMGFGHRVYKISDPRSDVIKAWAK
                     KLADAQGDKKLYAVSERVEQVMRREKNMFPNLDFYSASAYHFCGIPTAMFTPLFVVSR
                     ITGWAAHIIEQRADNRLIRPNADYVGPEPRVYVPMADR"
     gene            6942..7946
                     /locus_tag="VF182_13185"
     CDS             6942..7946
                     /locus_tag="VF182_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016716533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HEX6438085.1"
                     /translation="MPLPQTFKAMVVSETADKKFSREIKQVALSDLPAGELVIEVKYS
                     SLNYKDALSATGNKGVTRKYPHTPGIDAAGVVVNSTSRDVAVGSEVIVMGYDLGMNTA
                     GGFGQYISVPAGWVVKLPAGLTLRDSMAYGTAGFTAALCVMRLLAGGLNKEAGEVLVT
                     GATGGVGSVAVGILAKLGFDVVAATGKTAEKDFLTGLGAKTIISRDEANDTSGRPLQK
                     GRWAGAVDTVGGNILATALKTAKYGGLVTACGNTMSADLSVSVFPFILRGVSLLGVDS
                     VEIPMATRARVWQKLASEWKLDMSGDLITECSLEELNPKIEEILKGAIRGRVIVNPSK
                     "
     gene            8183..10588
                     /locus_tag="VF182_13190"
     CDS             8183..10588
                     /locus_tag="VF182_13190"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6438086.1"
                     /translation="MTNDSKTNSESALSHLRVIELGDIPASYAARWLADLGADVIKVE
                     PPGGDPGRLLPPFAGNIEDRERSLTFIHANTNKRSIVLDLQREADRETFARLLASANL
                     LIEATPLGFLEKFGFTDERFANDFSHLVVVSLTPFGRTGLYRHYKGSDAIANATGGFL
                     FGQGDNTKGQCTAPSHLAYQMAGAMAAMLALAGIRHSRETGAGQRIDVSLQEALTFTN
                     SGSIARYSRENRLERRPGGKAYGGAGTNIYRCKDGRYVHFTTNMPHMWKEFAQNWMTD
                     KALAGPEWENPRYRDQHGEEVAKAFANFIGQFDADDFADQAQARHLAAAPLNTIGEFV
                     DCEQNRARGWLQELEHPVIGKYKAPGAPMRLSLTPICVRRPAPLLDQHRKEILAELEK
                     LAAGKSTNQSDANRTRQPMLAGLRMADLTQQYAGPLGTELLAYYGMEVVKIESAVIAS
                     KEREAAVHACMNRGKLGCTINLRSPEGKELFKQLVEKSDVVVDNFSSGVLERLGFSFA
                     TLQQINPGIVQVVMPGWGLTGPLKSWVAWGWQLLAYTGIMRLWGYPDSPMETRCKIAW
                     PDRVGSVTMALGVLAALEYQARTGRGQFIEAGMLEAQGSMMGPAILDYTINGNEWDAM
                     GYREILGEPYAPYGCYPCYGQDNWIIIACAGEKEWRSMVDLIGKSSWAADDRFATHKA
                     RKNYREELDQKLSQWTCKYTAKQLFRMLQESGVAAGIPSSGEALFHDIHLRERGHIVE
                     TEAQPWGKITHHGLPGVPSLSAASAARPAPWIGANNDQVFGEILGLSSEKVAELKETG
                     AIK"
     gene            10721..11344
                     /gene="pdxH"
                     /locus_tag="VF182_13195"
     CDS             10721..11344
                     /gene="pdxH"
                     /locus_tag="VF182_13195"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX6438087.1"
                     /translation="MHREHRTQTKDRGHGMNSDPILRFRALLAEASQLGIAPYNAAAF
                     ATTGKDLQPTVRMLLLKEIDNRGLVFYTNLESRKARQLSDNPRASACFWWPQLRQQVR
                     FEGAVEIVSGREADEYFASRPRGSQIGAWASHQSSELSSREELIAAAASLTAEYKDRM
                     VPRPPQWSGYRLLFERIEFWREQPDRLHDRELYTRQAEGWNVTLLAP"
     gene            11542..12426
                     /locus_tag="VF182_13200"
     CDS             11542..12426
                     /locus_tag="VF182_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6438088.1"
                     /translation="MKPAPFEYYAPLELEEALELLDRHGDEAKILAGGQSLMPLMNLR
                     LARPGVIVDINRLRNLDAISIAPDGGLTIGALVRQRTLERSSFVQEQNPILAAAMPLI
                     GHFQIRNRGTIGGSLVHADPAAELPAVSLLLGGEFLLKSRSGARHVAAADFFRGYLAT
                     ECRPGELLAEIHLPKWRSGEAWAIDEIARRRGDFALAGVALRAAPDGAGLLQSAGIVI
                     FGVGGKPQRMQRAEALLQGRRIDQTLLSELSRAVAEELEADSDVHASAAYRKEVGGVL
                     VRRALQSALLHAKETVSH"
     gene            12423..12920
                     /locus_tag="VF182_13205"
     CDS             12423..12920
                     /locus_tag="VF182_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6438089.1"
                     /translation="MSAKQVIKVTVNGRIYERLVEVRMTLADFIRQELDLTGTHLGCE
                     HGVCGACTILLNGEAVRSCLMLAAQADGAELRTVESLAEGDELHPIQRAFQDHHALQC
                     GFCTPGFLMTALAFLNDNPLPTRDEVREAISGNLCRCTGYQPIIDAILQASSELQSKS
                     TRPST"
     gene            12921..>14561
                     /locus_tag="VF182_13210"
     CDS             12921..>14561
                     /locus_tag="VF182_13210"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6438090.1"
                     /translation="MKAMDKAAQGYVGMPIKRREDVRFITGRATYVDDIKLPNMLHAA
                     ILRSPRAHARIRSMDTSKALQIRGVIKIFTFQDLAELAVPVPIRMYQLPGLERHLQPL
                     LAHDTVRYVGEPLALAVAESRYLAEDALDAIDVEYQDLPAVVDVYEALKDEVLVSEEA
                     GTNLAAVHEFTIGDVERAFQEAQYTRREEFRVHRFTGNPMETRGFVAAFDRGKGDLTV
                     WGVTKLPHLNRQTIAAFLKLPERKLHFIENDVGGGFGIRGELYPEDFLIPFAAVKLGQ
                     PIKWIEDRREHLMAANHSRENRCELEVAARNDGTILAFRAVVYGDMGAYVRTHGGLVP
                     CSTAALLTGPYRIPNYRAKIHLVVTNKTGLGTFRAPGRYESCFYRERMLDLMAEDLKI
                     DPVELRLRNLIRPDEIPYEVGITRPGSGPTILDSGNYPSALKRGLEEFGYEKLQPLQG
                     RLENGRYHGTGIACFVKNTGGLEPYEGARIVIGDRNDVAVYLSISVLGQGHETAMAQI
                     CADALGVPMDWITVYHGNTDTTPFGWGTFASRGTVMCGS"
BASE COUNT         3150 a         4049 c         4249 g         3113 t
ORIGIN      
        1 ttcccgtttt cacgggaatg acgacgaaag catctaagaa tctgcaacga acttctaggt
       61 gatactaaat cgacttgcgc acctctttgg caacatcgac gagtagcttt gctttgacat
      121 cggcgagttc ccggctgatg gtcatcaagc cgcagtccgg cgccaacagc agatttgccg
      181 gatcgatgta tttcagagcg tggcgcacgc gctggacaac cgtttcaacc ggcggcaccg
      241 gggcatcact gggatcgacg cagccgaaca ttatctttcg acccttgcag atttccagca
      301 ccgctggatc ataaccgctt ctcgcaaact cgacggcgaa accgccgatt ttggttttca
      361 ttaactcttc cagcaattcc ggatactcgt actgatgttg ccgatcagct ccgccgggat
      421 agccgtagca gagatgcaca atggtcggaa tccgaatgtc ttggaggcaa cggtccaacg
      481 cccgggcgcc atacgcaaac accttttcat ggtagcgggt catcgccggc tcgtcgactt
      541 gcaagacgtc gcagccgaac gcttgcagct ccaacagctc tgaatttatc gcggccgcga
      601 tgtccatggc gagagcgggt tcgtcgttat aggtctcgtc caatgtgctg tcgatcaccg
      661 tcatcggtcc cggtacggcc attttgatcg gtttgctggt ttgccgcctg gcgaaacgca
      721 agtcttcgac gttgaccgac ccccgtcgtt tgaccttgcc gatgatgcgc ggcaccaggt
      781 gttcctgctc acggcggcga tagatctttt taactgcctg gttttcgaca tcgatcccat
      841 cgaacgcggc aaggatgtgg tgaataaagc cgatccgtcg ttgttctccg tcggtcagca
      901 gatcaagacc gatctgttgc tgggtctgca ggatcaagcg agtggcatcg tcctgcgcct
      961 ctttgagcgc cgtgccttgc caatgaaatt tgacctggct gcgttccgat ttcgccagcc
     1021 aagcgggacg gggaaaactg ccgacggtcg tggtggctac gggtggaagt tgcatgggtt
     1081 tgcctccgaa aggttgctcc aaatctatac tgagccgcgc ccttaaagac aaccttttct
     1141 tgaaatacgt tctgtagtag cgtcggcaca gagccgaaag gggaaaccga tggcggtgtt
     1201 aattaaagga ttaaaaaacg gtccattgga aaatctccgg cgcggtgaaa gtattggact
     1261 ataagggcaa agagtacgcc gatgccggag atccgattta tctctgtcgt tgccaatcaa
     1321 agagcaaacc ttagtgcgat ggcagccctg cagtggtagg cttcaagtcc gaagatatag
     1381 ccgcctaaaa gctgtgcggc gaggaaaggg gcagatgcgc ggctgatcga gctgtcttga
     1441 atctaatcat cataaacttg gatcaagtta atgggaggtc tcgctatggc tacgactaac
     1501 gaacttcgat tgtatttgct gacggctgaa ggactgatcc gctgcggctg ggacggccgg
     1561 tccgagcatg ttgaaattct caatagcgcg ttggctggag aagttctgcg tgaagtcgcc
     1621 caggatcctt tcgatcgcaa tcacctttat gcggcgacga tcacggaaat ccacgtcagc
     1681 gaagatggtg gcgcgcattg gaaatggctt ccctccggcg gtatcgatta ccgtgacatc
     1741 tgggcgatgg atgtcgaccc cactcgccgc gaccaaatct atgtcggcac gttgccggcc
     1801 gctgtctacg tcagcgaaaa tggcggccgc tcgtttcgag agctctcggc atttcgcaaa
     1861 ctccctgaat acggcaaatg gacctttccg ccggcgccgc actcggctca catccgttgc
     1921 tttgcgctgg atgcgcgttt tccggacgag atcatcgtcg gtatcgaaga gggcggcatc
     1981 gcgcgcagcc gcgatcgtgg cgagagttgg caggatatca gcggtccgca gagtgcgatg
     2041 gcgtatccgg agtacaacga tccggccggc atcaagccct accaaatggg acagcatgaa
     2101 gacggtcgag tctaccgcga tgtgcactgg gttctgcgcc atccgcgcag cgtgaatcgg
     2161 ctttacgcaa ccaccggcat cggcacttac cgctccgacg atgacggtaa atcatggatc
     2221 aaggcggagt atggtatggg tcgggcgtac gccattccga tcgcgatcca tcgcgattcg
     2281 ccggacagaa tctttcttgg cgccgctgaa aatggaccga catcatggct cggtcaccgc
     2341 acggtgcgcg ccgggccgta caataccatc aagttcagtc ccgacacttc ggagaaacta
     2401 ggcggcgcga aaaccggaat cttgcgcagc gacgacgccg gagaatcctg gcggcgcttg
     2461 aacaacggat tgccccagtc gtacccgcac atgacttgcg gctttgcttt gcatccgcag
     2521 gatggcaaca ccgtgtgcgt aagctatacc gacggttcga tctatgcgag caacgatgcc
     2581 ggcgaaagct ggcgtaagct cgacgtgact gtctcgaagc tctatgggat ccggctgatg
     2641 acagccaatt gatttgtgac agtcgctctt aaagatttag gaagatccgc tcgaacagct
     2701 ttccggctca ggaccattcg ctgagcgcca tgtactgttt ttccaatcgc tgccactcat
     2761 cgagaccgac gactttgtcg cgctctttga aagtggtcag cgcatcgtcc attttctcgg
     2821 tcgtgccctc gcgtttgagc agcccgagcg cctgtctcat ggcgtggatc gcggctcttt
     2881 gggtctccga cggatagata gcgatgcggt aacccatctg ctgcaggcgt tcaactggca
     2941 gcatcggcgt tttgccgcct ttgaaaacat tcaccagcaa gggcacgccg ggcagcgccc
     3001 gaggaatatc ctccagttcg gcttcggact gcggcgcttc tacaaaaacg gcgtcgactc
     3061 caagctcagc aaaggctctg cctctgcgaa gcgcatcatc taagccatgc acgccgcgcg
     3121 catcggtgcg cgcaatgatc aagaagtccg gatcgttgcg gctcgacagc gccgcctgca
     3181 gtttgttttc catttcggcc agcgggatta cttctttgcc atcgagatgg ccgcagcgct
     3241 tgggcgtgat ttggtcttcg atgtggattg cggcaacgcc ggtccgctcg aattctcgga
     3301 cgctgcggac caggttgact gcattgccgt agccggtgtc ggcatcggcg ataatcggaa
     3361 tttttaccgc ggcaaccact tgcgccgcgc gctcgatcac ctccgacata gtcaccaagc
     3421 cgatatccgg tatgcccatg ctccgcgcca ccgcgccgcc gcttaggtaa gcagcctcga
     3481 agccggcttc ctcgaccagg cgcgccgaca atccgtcgaa gcatccaggg gcgaccaaaa
     3541 ggcggtcacg tttaaacagc tgcttgagta attgtctcga attcattttc gttctccgat
     3601 ttttcttatc ataaaaagat tttcaaatgg cacagcgatt gaggacgagc cctccagagc
     3661 aaaggcaata ggtttttgcc taatagcggc ccgcgtcctt cagtggttta tcgggcgtcg
     3721 gaaaaattac tagctcaggg ccaaacaccc tagacgacaa tgaaatagtt cgcctcgtgc
     3781 cttcgctcct ccggactcac cttcaatcga tgcaccgttg tccatctccc ctgcaacttt
     3841 gggaataaat tcatgcagac tctacagtga acatgtccgt gaaaacgtcg accggagtcg
     3901 agtgcaatgt cttcaagtcc aaacacagtg cgagaatctt atcgcaccac gcaggcgcca
     3961 gccgcgaagt cagattggtt tcgaattttt tgattagcag cggcatgcct tcggctcggc
     4021 gccggcggtg gccgaccggg tactcgaccg cgatattttc actggccgag ccgtcggtga
     4081 aaaagacctg cactgcattg gcaattgagc gcttatccgg atccagataa tctttggaat
     4141 aacgcgggtc ttcgacaact tccattttga ttcgcaaagc atcgatgcgg gggtctttgg
     4201 cgatggcatc ctggtaatga tccgccgtaa ggtcgccgaa gatgagaccg atggcggtca
     4261 tatactgcaa acagtggtcg cgatcggctg gattgtagag cggtccggtt ttatcgatga
     4321 tgcgtatcgc cgactcctgc gtggtaatcc ggaccttctc gatgtcttca agtcgatgag
     4381 cgacttcgcg gtgcagccgc aaggcgcact ccacggcggt ttgggcgtga aattccgccg
     4441 gataggaaat tttaaaaaga atattctcca tcacatagga gccgaagggt cggcccagct
     4501 cgaatggatt gccgtgaaac agcacgtcat agaagcccca ctccttggcc gacaatgcgg
     4561 acggatagcc gatctcgccg cgcattgcca tcagcgcgag acgcactcct ctgctggtgg
     4621 cgtcgcccgc cgcccacgat ttgcgcggtc cggtgttggg cgcgtgacgg tatagccgca
     4681 ggctgccgcc atcggtccag gcattggaaa gagcgctgac gatctgctct cggctgccgc
     4741 cgagcattgc cgttgccacc gcggcggttg ccactcggac cagcaagacg tggtcgaggc
     4801 cgacgcgatt gaaactattc tccagcgcga ggacgccctg gatctcatgc gctttgatca
     4861 tcgcggtaag gacattgccg accgtgaacg agcgttgccc cgagcgagtc aaataatcgg
     4921 ccagagctag tatcccgccg agattgtccg aggggtgccc ccactcggcg gccagccagg
     4981 tgtcgttgaa atcgagccag cgaatcatcg cgccgatatt gaaagcggcg tgtaccggat
     5041 cgagctgcca ggcggtgccg ggaacccggg cgccgttggc cagcaacgct ccgggaacaa
     5101 ccggcccaag ctggtttgtg cagtcggaat aacgcagtgc caggagcccg cagcccagcg
     5161 tgtccataaa acagtatcgc gccgtatcga tggcctcggc gctgctggca tcgtaatcag
     5221 ctgcgtaggc ggcaatctct agcaaaagcg catcggagga tggacggata ttcaatgtcg
     5281 ttgaagtcat aaggctaatc tgaggttttt accgcaaata acgatcaagg aacgaacttt
     5341 caggagcaaa ggcaatgggt ctccatctac gtctccgctc ctccaagctc actccttgat
     5401 cccatcgtga cgcatgttcc tcgaaggatg gtgcatcgaa gctaactagc ggtttgttga
     5461 aagctctgat taaaggctgc tcaaaagact cagaggcgag acgggcgaaa aatcggcgag
     5521 cgaaggcgta ctcttcagta cgtcgacgcg agacgattgg agcccaacga agcatatgag
     5581 tccttttcag cagcctacta acgatccgcc atcggcacat agacccgggg ctcgggcccg
     5641 acgtaatccg cgttgggtcg gatcagccgg ttatcggcgc gctgttcgat aatatgcgcg
     5701 gcccaaccgg tgatgcgcga gaccacgaac aacggcgtga acatcgccgt cgggatgccg
     5761 cagaaatgat aggcgctggc gctgtagaaa tccaaattgg gaaacatgtt tttttctcgc
     5821 cgcatgacct gctcgacccg ttccgatact gcgtagagct ttttgtcgcc ctgagcatcg
     5881 gcgagctttt tcgcccatgc tttgatcaca tcggagcgcg gatcagagat tttatacaca
     5941 cgatgaccaa aacccatgac cagctttttg gcgccggtca tttcgagaat tccgctttca
     6001 gcttcctccg gggtggaaaa cttttcgagt aactccatgg cagcctcatt ggcgccgccg
     6061 tgtaggggac cgcgtaaagc gccgatggcg ccggttactg cggaataaaa atcaggcaga
     6121 gtagaggcga tagtgcgcgc ggtaaaggtc gaggcattaa attcatgctc ggcataaaga
     6181 atcaaagaga cgtcgacggc gcggcggcat agctcgtccg gctttttgcc gtgtaaaaga
     6241 tgcaaaaaat ggccggcggt gctatcgtct tcggttttgg tatcaatgcg tttgccggta
     6301 ttgacgtatt gatgccagta caaaagcatc gagctgaagc atgcggtcag ccggttggcg
     6361 atggcgatct gatcgtgttc cttattttcg ggctcgagag ttccgagcat ggagcaggca
     6421 gtgcgcaaaa cgtccatcgg gtgcgtgctg ccgggtatct gttcgagcac agctttgagt
     6481 ggcgaagata atccgcgctg cttgcgcaaa ttctccttgt actcgcgcag ctgtgatccg
     6541 ctcggcagtt tgccgtagat caacagatac gcgacctctt cgaaggtcga gtgctcggcg
     6601 aggtcataga tcgaatagcc gcgataggta agtcctttgc cttccttgcc gacggtcgca
     6661 atcgatgtgt taccggcaat aattccagct aagccacccg ttttgtcggt tgccataaga
     6721 attcctccag gccccacttt gccctgaacc gaatacctat tgcaagcaag ttgtccgact
     6781 tcttctatcg attttgcgcg cgaatctttg cttcggagac gacttggctc gagtctggct
     6841 cgggcggcgc aattcttatc ggggctaccc cttgacagtt tgcgctgcag attatgtact
     6901 cgcttcttgt gactatcttt tacggatgag gagtgatcag catgccttta ccacagacgt
     6961 tcaaagcaat ggttgtttcc gaaaccgcgg ataaaaaatt ttcccgagag ataaagcagg
     7021 ttgcactgag cgatttgcct gccggtgaac tggtcatcga ggttaaatat tcctcgttga
     7081 actacaagga tgcgctatcc gccaccggca ataaaggagt tactcggaag tatcctcaca
     7141 cgccgggcat cgatgccgcc ggcgtggttg tcaactccac cagcagagac gtcgccgtcg
     7201 gtagcgaggt catcgtcatg ggttacgatc tcggcatgaa taccgccggc ggcttcggcc
     7261 agtacatcag cgtgccggcg ggctgggtgg tgaagttgcc ggcggggtta actttgagag
     7321 acagcatggc ctacggcacc gccggattta ccgcggcgtt gtgcgtgatg agattactgg
     7381 cgggaggtct caacaaagag gcgggcgaag tgttggtgac cggcgccacc ggcggagttg
     7441 gcagcgtcgc ggtgggcatt ctcgccaagc taggattcga cgtggttgcc gccaccggaa
     7501 agacagccga aaaagatttc ctcaccggcc tcggcgccaa aacgatcatc tcgcgcgatg
     7561 aggctaatga cacctcagga aggccgcttc agaagggccg ctgggccggc gcagtggata
     7621 ccgtcggcgg caacatcctg gcgacggcgc ttaagacagc aaaatacggc ggcctcgtca
     7681 ccgcatgcgg caataccatg tccgcagatt tgtccgttag cgtctttcca tttatcttgc
     7741 gcggagtcag tttgcttggc gtggattccg tcgagatccc gatggcgacg cgtgcgcgcg
     7801 tgtggcagaa gctcgccagc gaatggaagc tggacatgtc gggcgatttg atcaccgaat
     7861 gttcgttgga ggagttgaat cccaagatcg aggaaattct caaaggcgca attcgcggca
     7921 gggtgatcgt caatccgagc aagtaatttc cggcaggctg ctgaaaaaac ccataggttt
     7981 cgttgcgctc gcccgattcg cttcaacgta ctgaagcgta cgcctcagct catcgaactt
     8041 cgcgcgcctc acctctggat ctttttgagc agcctgcaag aggtttttaa caaccgctag
     8101 actaacattt atgcatttgt tctttccgtc tcctgtttgg ggatggaagg cgtgagggct
     8161 tgaagaaccg aaaggatagc aattgacgaa cgacagcaaa accaattctg aatcggcgtt
     8221 aagccacttg cgcgtcatcg aactcggtga catccccgcg tcttatgcgg cacgttggct
     8281 ggcggatctc ggcgccgacg tgatcaaagt cgaacctcct ggcggcgatc ccggccggtt
     8341 gttgccgccg tttgccggca atatcgagga tcgcgagcgc agcctgacgt tcattcatgc
     8401 caataccaac aagcgcagca tcgttctcga tctccaacgc gaagccgacc gtgagacctt
     8461 cgcccgcttg ctcgcttccg cgaatctgct gatagaagca actccgcttg ggtttctgga
     8521 gaagtttggc tttaccgatg agcgcttcgc caatgacttc tcccatctcg ttgtagtttc
     8581 gttaacgcca ttcggcagaa ccggactcta ccggcattac aaaggcagtg acgcgatcgc
     8641 caacgcgacc ggcggatttc tttttggcca gggcgacaac accaaagggc agtgcacggc
     8701 accgagccat ctcgcctatc agatggccgg cgcgatggcg gcgatgttag cgcttgccgg
     8761 aatccgtcac tctagagaaa cgggcgcagg ccagcgaatc gatgtctcat tgcaagaagc
     8821 gttgacattc accaacagcg gctcgattgc gcgctatagc cgcgaaaacc gcctcgagcg
     8881 ccgtcccggc ggcaaagcat acgggggcgc aggcacgaat atttaccgct gcaaggacgg
     8941 ccgctatgtc catttcacca ccaacatgcc gcatatgtgg aaggagttcg cgcaaaattg
     9001 gatgaccgat aaagcgctgg ccgggccgga gtgggagaac ccgcgctacc gcgatcagca
     9061 tggcgaggaa gtcgccaagg cgtttgccaa tttcattggt cagttcgatg ctgatgattt
     9121 tgcggatcag gcgcaggcgc gccatctcgc cgccgcgccg ctcaacacga tcggcgagtt
     9181 tgtcgactgt gaacaaaacc gcgcgcgcgg atggttgcag gaactcgagc atccggtgat
     9241 cggcaagtac aaggcgccgg gcgcgcctat gcgtctctcg cttacgccga tatgcgtgcg
     9301 ccggccagca ccgttgctgg atcagcaccg aaaggaaatt cttgcggaac ttgaaaagct
     9361 cgctgccggc aaatcaacta atcagagtga tgcaaatcga acgcgtcagc ccatgcttgc
     9421 cgggctgcgt atggccgatt tgacgcaaca gtatgcgggc ccgctcggca ccgaactgtt
     9481 ggcctattac ggcatggaag tcgtgaaaat cgaatccgca gttatcgcca gcaaagagcg
     9541 cgaagcggcg gtgcacgcct gcatgaatcg cggcaagctc ggctgcacca tcaacctgcg
     9601 cagccctgag ggcaaggaac tttttaaaca actggtcgaa aagtccgatg tagttgtcga
     9661 taacttcagc tccggcgttc tggagcggct gggcttcagc ttcgcgacat tgcagcagat
     9721 caatccggga attgtccaag ttgtgatgcc gggatgggga ttgaccgggc cgctgaagtc
     9781 gtgggtcgcc tggggctggc agctcctggc ttacaccggc atcatgcggc tctggggata
     9841 tccggattcg ccgatggaaa cccgttgcaa gattgcgtgg cccgatcgtg tcggctcggt
     9901 gaccatggcg ctcggtgtgc tcgccgcgtt ggaatatcaa gcgcgcacgg gcagaggcca
     9961 gttcatcgaa gccggcatgc tcgaagcgca gggctcgatg atgggacccg cgattctcga
    10021 ctacacgatc aacggcaacg aatgggatgc gatgggctac cgagaaattc tcggtgaacc
    10081 ctacgctccc tacggctgct atccctgtta tggccaggat aattggatca tcatcgcctg
    10141 cgccggggaa aaagagtggc gcagcatggt ggatttgatc ggtaaatcat cctgggccgc
    10201 tgatgacaga ttcgctaccc acaaagctcg caagaactat cgtgaagagc tcgatcaaaa
    10261 actttcacaa tggacatgta aatatacggc aaaacagtta tttcggatgc tgcaagagtc
    10321 tggtgtggca gctggcatcc catcctccgg cgaagcctta tttcacgaca tccatctgcg
    10381 cgagcgcggc catatcgtcg aaactgaagc gcagccctgg ggcaaaatca cgcatcacgg
    10441 gctgccggga gttccttctc tttccgccgc cagcgcggcg cgtccggcgc cctggatcgg
    10501 cgccaacaat gatcaagtgt tcggtgagat tctaggtttg agtagcgaga aagtagccga
    10561 gttgaaggaa acgggcgcga tcaagtaatt gtcgtaggct cgacagctaa tcgcaaagtt
    10621 cacagctcaa gacagatgag tccatggatg actaactgga catgtcgcga tactcttcgg
    10681 gagccgttcc tgcagccgac ctgatcaacc gtgatggcaa attcatcgag agcaccgaac
    10741 tcagacgaag gatcgaggac acggaatgaa tagcgaccct attctccggt ttagagcatt
    10801 gttagccgaa gcctcacaac tcggcatcgc gccgtataac gcggcggctt tcgccacgac
    10861 gggtaaagac ctgcaaccca ccgtgcgcat gctgctgttg aaagagatcg ataatcgtgg
    10921 acttgtcttc tacacaaatc tcgagagccg caaggcgcgc caactgagcg ataatccgcg
    10981 agcatccgcc tgtttctggt ggccgcagtt gcgacagcaa gtgcgcttcg agggtgcagt
    11041 ggagatcgtc agcggccgag aggccgacga gtacttcgcc agccgtccac gcggaagtca
    11101 gatcggcgcc tgggcatccc accaaagcag cgagctctcg tcgcgcgagg agctcattgc
    11161 cgcggctgcc tctttgaccg ccgagtacaa ggaccggatg gtaccgcgtc cgccgcagtg
    11221 gtcgggatac cggctgttgt tcgaacgtat cgagttctgg cgagaacagc ccgaccgttt
    11281 gcatgaccgc gaactttaca cgcgccaagc cgaaggttgg aatgtcacac tattggcgcc
    11341 ataaggaatg ctgcttccgg ctctttgttc agagcgatat tgagcagaag agtaagcagc
    11401 aaccgtttga agtgacaccg caagctaagc ctcttgatat ccgcttaccg ctgaaaaccg
    11461 atcgaattcg ccacggaatc gcgcgccgac tcgctttatt tgctgctcat cgacaagttt
    11521 gatatctggt cggtgacagt tatgaagccc gcgccattcg aatactatgc gccattagag
    11581 ctggaagagg ctttggagtt gctcgaccgc catggcgatg aggcgaagat cctggccggc
    11641 gggcagagcc tcatgccgct gatgaatctg cgactggcgc ggccgggcgt cattgtcgac
    11701 attaaccggt tgcggaatct cgacgcgatc tcgattgcgc cggacggcgg gctcacgatc
    11761 ggcgcgctcg tacggcagcg gactctggag cgttccagtt tcgttcagga gcaaaatccg
    11821 attctcgccg cggcgatgcc gcttatcggc cactttcaga ttcgcaaccg cggaaccatc
    11881 ggcggtagtt tggttcatgc agatccagcg gccgagcttc cggccgtgag cctgctcctc
    11941 ggcggcgagt tcttgctgaa gagtagatcg ggcgcgcgtc atgttgcagc ggcggatttt
    12001 tttcgaggat acttagcgac cgagtgccgg ccaggagagt tgcttgccga aattcacttg
    12061 cccaaatggc gctcaggcga agcatgggcg atcgatgaaa ttgcgcgacg gagaggtgat
    12121 ttcgcgttgg ccggggtggc cttgcgagcc gcacccgacg gggccggtct gttgcagagc
    12181 gccggcattg ttatattcgg agtcggtggc aaaccccaga ggatgcaacg ggctgaagcg
    12241 cttttgcaag gacggcgaat tgaccaaacg cttttaagcg agttgagcag agccgtcgcc
    12301 gaagaactcg aagcggattc ggatgttcac gcttccgctg cttaccgcaa ggaagtaggc
    12361 ggcgtattgg tgcgccgggc tctgcaatcg gccttgctcc atgcaaagga gacagtgagc
    12421 cattgagcgc aaagcaagtc atcaaagtta ccgtcaacgg acgaatctac gaacgtctgg
    12481 tcgaagtaag gatgacgttg gcggatttca tccgccagga gctggatctc acgggcactc
    12541 atctcggctg cgagcatggg gtatgcggcg cctgcacgat cttgttaaac ggcgaagcag
    12601 tgcgttcgtg cctcatgctc gcggcccaag ccgacggcgc cgagttgcgc actgtggaga
    12661 gtcttgccga aggtgatgag ctgcatccga tacaacgagc ctttcaggat catcacgcgc
    12721 tgcaatgcgg cttctgcaca cccggttttc taatgacggc attggccttt ttgaacgaca
    12781 atccgctgcc gacgcgggac gaagtccgcg aggcgatttc aggcaacctg tgccgctgca
    12841 ccggctatca accgattatc gatgcgatcc tgcaggcctc ctcggaattg caaagtaaat
    12901 cgacccgtcc gtcgacctag atgaaagcca tggataaggc agctcaaggt tatgtgggta
    12961 tgccgatcaa gcggcgcgaa gacgttcgct tcatcaccgg gcgggcgaca tacgtcgacg
    13021 atatcaagct ccccaacatg cttcatgcgg ccatcctgcg cagtccgcgg gcgcacgcga
    13081 gaatccgctc gatggacacg tcgaaggcct tgcagattcg cggcgtgatc aagatattca
    13141 cgttccaaga tctcgccgag ctggcggtgc cagtgcccat tcggatgtat cagcttcccg
    13201 gactggaacg gcacctgcag ccgcttcttg cgcacgatac ggtccgctat gtcggcgagc
    13261 cgctggcgct ggctgttgcc gagagccgat accttgccga ggatgccctc gatgccatcg
    13321 acgttgagta ccaagacttg cccgcggttg ttgatgtgta cgaagcgctt aaagatgaag
    13381 ttctggtgag cgaagaagcc ggaacaaatc tcgcggcggt tcatgaattt accatcggcg
    13441 atgtggagcg cgcgttccaa gaggctcaat acacccggcg ggaggagttc cgcgtccacc
    13501 gctttaccgg caatccgatg gagaccaggg ggttcgtcgc cgctttcgat cgcggcaaag
    13561 gggatctgac cgtttggggc gtcaccaagc tgccgcatct caaccggcag acgatcgccg
    13621 cgtttcttaa actccctgaa cgtaaactgc actttatcga aaacgacgtc ggcggcggct
    13681 tcggcatccg cggagagcta tatccggaag actttttgat cccgtttgcc gctgtaaagc
    13741 tcggacaacc gattaaatgg atcgaagatc ggcgcgagca tctgatggcg gccaatcatt
    13801 cgcgtgaaaa tcgctgtgag ctcgaggtcg cggcgcgcaa cgacggcaca atcctggcgt
    13861 ttcgcgcggt cgtttacggc gacatgggcg cctacgtgcg gactcatggc gggctggtgc
    13921 cgtgttcgac agcggcatta ttaacgggcc cgtatcgaat accgaattac cgagccaaga
    13981 tccacttagt ggttaccaac aagaccggcc tggggacctt tcgcgcgccg ggccgctacg
    14041 aatcctgctt ctaccgtgaa cgcatgctcg acctgatggc ggaggatttg aagatcgacc
    14101 cggtggaact acgcttgagg aatctcatcc ggccggacga gataccttac gaagtaggaa
    14161 ttacccggcc gggctccggg ccgacgatac tagatagtgg gaattatccg agcgcgctaa
    14221 agcgcgggct tgaagaattc ggctatgaaa aattgcaacc gcttcagggt cgtcttgaaa
    14281 atggtagata tcatggcacc ggcatcgcct gcttcgtcaa gaacaccggc ggcctggaac
    14341 cttatgaggg tgcgcgcatc gtaatcggcg atcgcaatga tgtcgccgtc tatctcagca
    14401 tctcggtttt gggccagggg cacgaaaccg ccatggcgca gatctgcgcc gatgccttgg
    14461 gcgtgccgat ggactggatc actgtttatc acggcaatac cgacacaact cctttcggtt
    14521 ggggaacgtt tgccagccgg ggaaccgtca tgtgcggcag c
//
LOCUS       DASUVS010000241        18884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1411145, whole genome shotgun sequence.
ACCESSION   DASUVS010000241 DASUVS010000000
VERSION     DASUVS010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 18884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18884
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411145"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VF182_13215"
     CDS             <1..234
                     /locus_tag="VF182_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX6438091.1"
                     /translation="ASEAYLIEDGKVTEPVKGATLIGNGPDVLTRVSMVGTDLKLDEG
                     VGTCGKDGQSVPVGVGLPTIKIDGLTVGGTATQ"
     gene            273..1628
                     /locus_tag="VF182_13220"
     CDS             273..1628
                     /locus_tag="VF182_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX6438092.1"
                     /translation="MERLDLSQELAREVLAKAQAKGATQGDVVMAEGDSFFVTVRLGE
                     IEKISQAQEKRLGLRLFYGNSSASASTSDISRQAIDRLVDDTINMARFTAQDPFSGLP
                     DASLLARDIPDMDLFDGAGGSVSVDDKIAIALAAEKAAMEFDPRITNSEGAEFSNQSG
                     RIIYASSHGFCGEYHGSNFGHSVAPVASQNGSMQRDYWYSSNRKFARLDSAESVGKRA
                     AQRVLRRLGARKIKTCEVPIIFDPDMAASLMRNLASALSGYSLYKGASFLNGQLGNKI
                     ASDLTTVWDDGTIPGALGSKPFDSEGLPITKKAVVENGFLRSYLLDTYSGKKLNMAST
                     GNASRSIGEPPGVAPTNFYLAPGRQSPDEIIGSVKQGLFLTETIGFGVNLVTGDYSRG
                     AAGIWIEDGELTYPVEEVTIAGNLKEMFQNIEMIGNDLEMRGRIAAPTIKISKMTVAG
                     E"
     gene            complement(1789..1998)
                     /locus_tag="VF182_13225"
     CDS             complement(1789..1998)
                     /locus_tag="VF182_13225"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEX6438093.1"
                     /translation="METKTAIISIDELKSDDLPRLREALETLPGVQSLDFSLERMVAV
                     IDFDPKQSHLDDFLRAVLKAGFKVS"
     gene            2219..2656
                     /locus_tag="VF182_13230"
     CDS             2219..2656
                     /locus_tag="VF182_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020405583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438094.1"
                     /translation="MAYDVEKLLSMSQADLDTLFGKCAAGDIPNGEADGTAIVAPGTK
                     FSSNIAKFISNFAWQGKVFDAGKGVLKNRILPFGLNAIIAKVYKGPSWLDGKDCIVLD
                     YSDTSIIAQWIRDEIRQIEPGLYLGKVYWEKKRLIDFALKFNS"
     gene            2868..4388
                     /locus_tag="VF182_13235"
     CDS             2868..4388
                     /locus_tag="VF182_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6438095.1"
                     /translation="MAQPELASPGERILKRIGWITVVALVLILTAGALLLGRFGADTP
                     VAYKDDLEHFKYGSLGSEHEFGVPYWIWRALPELFEDKLPQPGRGWESVGFVFEKGKN
                     LPVGMSQRRYLGFDVVWLNCAFCHAGTVRESAESEPKVYSAMPANTFDFRAFMRFLFA
                     TGEDRRFTPRDILRQIRIIRQREGLDDLPLIDRLVLRFYGIYFMRERILTLRDRLDFI
                     KEQPEWGPGRVDTFNPLKAYFNFPPDQLSEEERIGTTDFPSIWNQGQRESLKMNLHWD
                     GNNVSLEERNRSAAMGTGITPPTGDRTSLKRVADWVRNLAAPDYPFGINKDLAAQGAP
                     IYQEYCAKCHGADGKDFRGEDVGKVVPIADIGTDRRRLDSYTYDVAVNQNMIFAGYGE
                     ERFSHFRKTFGYANSPLDGLWLRAPYLHNGSVPTLRDLLEPSDKRPKSFYRGYDVYDR
                     KKVGFVGDVSEEKGRKYFRYDTAEPGNSNQGHQGKRYGTELPAAEKDALIEYLKTF"
     gene            4424..5884
                     /locus_tag="VF182_13240"
     CDS             4424..5884
                     /locus_tag="VF182_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438096.1"
                     /translation="MVAGQKKSWWKTCLVLVLLFVAVVGIIGWYKFLREEPQPSWVTA
                     TPDMRFKYGSIGAEHDAGIPYWIFFVLPRMFPEKLPGPGGYASLGVAWEQGQELPIGF
                     TKKVIGFPRVANNCASCHTASYRKSADQNPVFVPAGPNHTLNLQAFFRFIVDCARDPR
                     FNADEIMSEIALVYDLPLLDRLIYRYALIPITKKRLLEREAQFKWVYHKDFPLWGRGR
                     DDAMNLTKYFLLNWPMDDTIGPTDMPSVWNLKKYQPEKGMLMNLAGDSHDARSVIIDS
                     ALGLLNAEPHDRDDFIGQIDWLVKYLSELQAPKYPFAIKADQAQTGKAVFDSNCAMCH
                     ASERTGTRVPVEEVGTDRERLKTWSKQAAIESNKVVRNFGIERRGLVENEPSGYIAAF
                     LDGIWLRSPYLHNGSVPSLRDLLKPAAQRPKVFYRGYDVYDPENVGFVTQGAEAQRVG
                     TKYDVDERASGNQGHEYGVNLSPAEKDALIEYLKSL"
     gene            5968..8364
                     /locus_tag="VF182_13245"
     CDS             5968..8364
                     /locus_tag="VF182_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438097.1"
                     /translation="MENCRLNIIAIVILVTLAAAACGKGSPEPGSVKDEALTVGRTAE
                     SLPAADEDYFKDMDGGVELTVNEVKGRNNWIVWSGGNDRFWDHLVSKSFGAVDFLKIL
                     SSHPNIKHLSRDTRWHYLGLVNEPCFEKPQTANKDRFGLWLDTRSKDCPPDPFENEKK
                     YPGIQIGARGKNIPVGSLYGYASGIVGLRLFPNPDFDDAAQKKWDAEKFYNDPSYYND
                     KNLIRPYRVGMSCGFCHIGPSPVKPPADPENPKWENLNSNPGTQYFWVDRILFWQKDD
                     ANFIQQLLHTSLPGTLDTSFISSDYINNPRTMNAVYSVAARLKAAERWGEEKLAGGGV
                     KNKQFQDFKQTAALAQYHKPPDTVWTPRILKDGADSVGVLGALNRVFINIGLFSEEWL
                     LHFNALVGGKKITPIKIEDAEKNSSYWNATVAQTPDVALFFLRTARPDLLKNAPGGEQ
                     YLTKDQVQLARGKTVFAETCARCHSSKIPAAPAGVDERNWDQYWQWTKTDDFKKKMTE
                     MVMADDFLVDNYLSTDRRIPVTLLQTNACSPLATNGLGGNIWDNYTSQTYKYLPSVGK
                     ITVHNPIDGSPQEYEMPGNGRGYTRVPSLISLWSTAPFLLNNSVGRFNPSPSVEARIE
                     SFNDSIEKMLWPEKRDKDLILGDKVPGFIYRTTAPSYIKLAPGFLPASLNHLLSWNDW
                     GARWLPWLFSEEGVQIGPIPKGTPVNLLTNIDLESSKIDLAKLILRMKADLKAVEGKN
                     EQEAAEVFKNLVPDLLQVSKCPDFVVNRGHYFGTSLLKEETPLSDEDKRALIEFLRTF
                     "
     gene            8393..10246
                     /locus_tag="VF182_13250"
     CDS             8393..10246
                     /locus_tag="VF182_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX6438098.1"
                     /translation="MPAANAPSNPYDYIVVGSGAGGGTVAARLAEAGCTVLLLEAGGD
                     PLGIAGTRLPEDYQVPAFHPFASENAAMRWDFFVRHYGTDERQKKDAKFTAERDGVLY
                     PRAGALGGCTSHNAMILVYPHNQDWDDIAELTGDASWRAENMRRYFERLEDCRHRPPY
                     RWLKKILGLNPTRHGFDGWLTTERRDPRLVLGDRDMMEMIKRSAVRAFKEMDDPLTQV
                     RWSIKSQHDPNDWRLVQSNSFGVRYSPLTTRNQARIGTRERILDVAKRFPHKLHVEPH
                     ALVTRVLFDENNRALGVEYLKGERLYRAHGDPSSDAGEKCQAFASRETILCGGAFNTP
                     QLLMLSGIGPKKDLQSHGIKVRVDLPGVGKNLQDRYEVSVVNRMTQDWNVLAAAKFDT
                     SDAQYREWAADRKGVYATAGAALVVVKKSQPGRTVPDLFCLGLVGKFKGYFPGYSRLI
                     SAHHNYLSWVVLKAHTRNNAGTVSLRSADPRDTPEINFRYFDEGSDARGDDLKGVVEG
                     IKFVRQMTRALKSEKMVAEEELPGNQVQSDAELSDYARYNAWGHHASCTCSIGAIEKG
                     GVLTCDFKVHGTTGLRVVDASVFPRIPGFFIVSAVYMIGEKAADVILADNR"
     gene            complement(10494..13289)
                     /locus_tag="VF182_13255"
     CDS             complement(10494..13289)
                     /locus_tag="VF182_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEX6438099.1"
                     /translation="MKGTFLFCLMLALVLVGAYPLTRQTQVGSCIAYSLAGLREVAQQ
                     RSARFLGKVYEDSARCRGGESAVVWRNTPWIDWQRYAGAGGPESRTAGWGGKLGFFSP
                     DGRGINGALLDLEYQRLELLKFNLFDNNGTFETYIRESHSPSGHVSRMWPELRLPKDH
                     PLYADVGGNGPQLCSGELIRFRTITGICNDITNPLMGSSGQRFARNVDFESTFPELAV
                     DPIAANRHGGRIGLLKPDPQLISQKLFTRKGSLDGKCHSHGLPEQPDQAGCDYQKASH
                     LNLLAGFWIQFMTHDWFSHLDEGQNQSAMMGMGCDNHQASNDQSSSSSTKTQPVGCRA
                     ADRVEKSLIAEDIPAPTFSFGNNSYMTRAHRTTANKVTAWWDASQIYGYDDTSQRRVK
                     RDSRDPAKLLLDPARTGARENTQYLPILNSGDPMNPQWSGQEAVAFPDNWNIGLSFFH
                     NVFAREHNIFVDAFRLATTTDPDADSGLRRPAQPGRVVSNKEVAANELFEVARLVVAA
                     EIAKIHTIEWTTQLLYNEPVYLAMNANWSGLFDEHPLVNAALERVIQRFTRSADGKKS
                     TGWYAAFAAGPGIVGLGNTQADVNAGVNHFGSPFNFPEEFINAYRLHPMLPDFIEVRE
                     LNRDRNVVKSKIALSETLRGKATQAMHQHGPANWALSLGRQRLGKLTLRNHPRFLQSL
                     SMPRLKSGSGQIDVAALDLIRDRERGIPRYNEYRRQYGLKQLTKFDDFIDPRLAKNSG
                     EWTEQQKLTGLLREIYGQHRCDSSRIITTAQKNDDGSEIDDCLGHPDGTLVDNIEDVD
                     AMVGWLAEFARPHGFAISETQFQVFILNASRRLFSDRFLTSSFRPAFYTNLGYNWVNN
                     NGPGGKIFERKKTNGHTVEVSPLKRVLQRTIPELREELEPVVNVFDPWARDRGEYYSL
                     DWKPRRGAEADFSFKSN"
     gene            13398..14402
                     /locus_tag="VF182_13260"
     CDS             13398..14402
                     /locus_tag="VF182_13260"
                     /inference="COORDINATES: protein motif:HMM:NF015132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6438100.1"
                     /translation="MIRLDAQLFALYAKDCIAQEHVETADNHINDPTKFSRHNLLNMA
                     AIPAEFYALANAFLFAFHNMLTKKAMRYSNPATGVISSLLINIVFLWSVSILFVPLSS
                     LTSASILIFVVVGFFQPGLTRLLTYKGIDALGMAITDPIRATTPLFSAMMAIIFLGEQ
                     ITLPIIVATLMIIAGITLLSWRSGSVKLAGSAVFLWYPIAASALAGASQVVRKFGLAA
                     VPHPFLAAAVTASSSFVVSLLTLWYVEKSEETWKMNRQCFWWFLAAGITISLGMVCIY
                     YALDLGKVSVVIPISSTGPFFSLIFSSLFLRDVERVTGRIVISAAMIVGGVLLLTIWK
                     "
     gene            14796..15062
                     /locus_tag="VF182_13265"
     CDS             14796..15062
                     /locus_tag="VF182_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438101.1"
                     /translation="MNNLPGFGLESGAALAADAADQPGTYGSAFGFGRQSLGISAGHG
                     LALPVGGTDNPEMEDIQFVYVTPRWVSASRIPWEDVVGTGETSS"
     gene            15086..15403
                     /locus_tag="VF182_13270"
     CDS             15086..15403
                     /locus_tag="VF182_13270"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEX6438102.1"
                     /translation="MFEPKSGIAGGIAPALRYNFLTGTRFIPFLHAGVGFIALDADLA
                     RQSDGLNFILQSGVGLHYFLSQSTALTGEWRFHHISNANIHDRNAGINSSLFLLGLTF
                     FLR"
     gene            complement(15486..16190)
                     /locus_tag="VF182_13275"
     CDS             complement(15486..16190)
                     /locus_tag="VF182_13275"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6438103.1"
                     /translation="MKFETVIFDLFGTLVNHFPSSARQMHREMVAVLEVPNEEFNRLW
                     SRTTEMRVIGAFETVEASIQYVLDKMNAQARPEQIRNAVALRMKYIKEALRPKADAIT
                     TLKELRRRGYGLGLLSNCSVEIPLLWPETDFAGAIDATIFSCLARLKKPDERIYHLAC
                     EQLGSKPRSCLYIADGEEHELAAAARTGLQPVLIRSSSEESLSVLHTEAREWRGPAIS
                     SLTEALALAGIETSRK"
     gene            complement(16314..17348)
                     /locus_tag="VF182_13280"
     CDS             complement(16314..17348)
                     /locus_tag="VF182_13280"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6438104.1"
                     /translation="MKRKISCASFLLAIVLLPFLAVSTAYSQDTFFKGKTMRIIVGGP
                     PGGGFDTYARMIARQMSKHIPGQPTIIVDNMPGAGMMIAANHIYKAAKPDGLTIGHFT
                     GSQTISQILGQPGVEFDMRKFTYLGAPTADHFSCAFTKASGIVSTDKWLASPRPVKMG
                     AVGPGNLTDNTLRVLSTATNFPIQLVSGYKGTAPVRLAMEAGEVDGSCWGWDSIKSTW
                     SRAMETGDGVVVLQAAPKAHADLPKVPLAINFGKNDEGKQLIDVVIHGGNIVERSFML
                     PPGVPKDRIAAIKSAFDKTLGDPEFLAEAKKARLGVAPISGDELTRRVNAFFNLPEDM
                     KRKIKRVLYE"
     gene            complement(17381..18415)
                     /locus_tag="VF182_13285"
     CDS             complement(17381..18415)
                     /locus_tag="VF182_13285"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438105.1"
                     /translation="MKRYLINMYATIFCSVGVLIGWNTPAAAVAAPAKTVIGYATVTA
                     RLMPLWIAHEQGIFAKYGIDAQPILIRGAPTLVAGLASGDIHIGRTGGSAMLAAVAAG
                     HDFKFVASFSSRNTYDLVVRPNIKRAEDLRGKRFAVTSIGGTTWMGILLWLEHLGLDQ
                     QRDQIQMQVVGDQGIQAQAVENGLCDAAVLDGAFSRKLQQKGMTILHEYTEMTKLLAG
                     QSMVVPGALLQQKGDLAENYLKAEIEALAYSLAPKNKPAVLKSLMRHLRTDAAGAEDG
                     YLDLTRGVDRKPIPSVEGLRNVQRLLKTRTPKVGDLKVEDVIDGRIARRLEESGFIER
                     AFASQGASLK"
BASE COUNT         4185 a         5039 c         5282 g         4378 t
ORIGIN      
        1 gccagcgagg cctatttgat cgaggacggc aaagtgaccg agccggtgaa aggcgcgact
       61 ctgatcggca atggccccga tgtgcttact cgagtatcaa tggtcgggac cgacctgaag
      121 ttagatgaag gtgtcggcac ttgcggcaag gatgggcaat cagtgccggt cggtgtggga
      181 ttgccgacga ttaagatcga cgggttaacc gttggcggca ccgcgacaca gtgattggcc
      241 cgaaatagtc aggaagcaag cgggaaggaa acatggaacg actcgatctc agccaggaat
      301 tagcgcgcga ggttctcgcc aaagcccaag ccaaaggagc gactcaagga gacgtggtga
      361 tggcggaggg ggactcgttc tttgtcacgg ttcgtcttgg cgaaatcgag aaaatcagcc
      421 aggctcaaga aaagcgtttg ggattgcgtt tgttctacgg caatagctct gcctcggcgt
      481 cgacttcgga tatttcgaga caagccatcg accggctggt cgacgatacc atcaatatgg
      541 cccggtttac cgctcaagat ccgttcagcg gcttgccgga cgcgtcgttg ttggctcgag
      601 atattccgga tatggatctt ttcgatggcg cggggggttc agtttctgtc gatgacaaaa
      661 ttgccatcgc actggcggcc gagaaggcgg ccatggaatt cgatcctcgc attaccaact
      721 ctgaaggggc ggagttttcc aaccaatccg gtcggattat ctacgccagc agtcacggat
      781 tctgcggcga gtatcacgga tcgaatttcg gccattcagt tgctccggtg gcgtcgcaga
      841 atggttcgat gcaaagagac tactggtatt cgtccaatcg aaaatttgcc cggctggact
      901 ctgcggagag cgtgggcaag agagccgccc aacgggtatt gcggcgcttg ggcgctcgta
      961 aaataaaaac ctgcgaagtg ccgattattt tcgatcccga tatggcggct tcgctgatgc
     1021 gaaatctggc gtcggcactg tcgggatatt cgttgtataa aggcgcatcc tttctcaacg
     1081 ggcagctcgg caacaagatc gcctcggatc taaccactgt ttgggatgac ggaaccattc
     1141 ctggagcgct cggctcaaaa ccattcgatt ccgaaggttt gcccattacc aagaaagctg
     1201 tagtcgagaa tgggtttttg cgcagctatc ttcttgacac ttacagcggc aagaaattga
     1261 acatggcttc gaccggaaac gcttctcgct cgataggtga accccccggc gtggcaccca
     1321 cgaactttta tcttgctccc ggccgtcaat cgcctgacga gatcatcggc tctgtgaaac
     1381 agggcctctt tttaaccgag acgatcggtt tcggtgtgaa tctcgtaacc ggcgattact
     1441 cgcgtggcgc cgccggtatc tggatcgaag acggcgagct cacgtatccg gtcgaagagg
     1501 tgacgatcgc cgggaatctc aaagagatgt ttcagaacat cgaaatgatt ggcaacgatc
     1561 tcgaaatgag aggacgcatc gccgccccga cgattaaaat ttctaagatg accgtggccg
     1621 gagagtaatt gccgttcaag gttcaaccgt tcaatgccgg acccattcgg aatttgtgaa
     1681 aaatctccgg aatcgcttgc agcaaacaac gtccgggaga tggtactaaa gcgcttcgtc
     1741 ccatactgct taccttgaac cgttgaacct tgaacggttg aaaggctctt agcttacttt
     1801 aaatcccgcc ttcaaaactg cgcgcaaaaa atcgtcgagg tgagattgtt tgggatcgaa
     1861 atcgatcacc gcgaccatgc gttccaagct gaagtccaga ctctgaactc ctggcaatgt
     1921 ttcgagcgcc tcgcgcagcc gtggcaaatc atccgatttc aattcgtcga tagaaataat
     1981 cgctgttttt gtttccatgg gtttcacttg gctcatagct tgacaaaagt tacttcgatt
     2041 cacaattcaa cgtggaatgc ggggatactt tagttctcag ctagaatttt attcagccgt
     2101 ttggaaattt tcttgacgtc ggccgcagtc gtatgagatt agtcgtcgtt agttcggtca
     2161 gcccacaatg acatcttcgt agaatcttga aacgtatctg acggaaggag gaaactacat
     2221 ggcttacgat gtcgagaaac ttttgagcat gtcgcaagca gacctcgaca cccttttcgg
     2281 caaatgcgcc gccggcgata ttccaaacgg cgaagcggac ggcactgcga tcgtcgcacc
     2341 gggcaccaag ttcagctcga atattgccaa attcatcagt aactttgcct ggcaaggtaa
     2401 agtattcgac gccggcaaag gagtattgaa aaaccgaatt cttccttttg gtctcaacgc
     2461 catcatcgcc aaagtttaca agggacctag ttggctcgac ggcaaagact gtattgtttt
     2521 ggattactcg gatacttcta tcatcgccca gtggatacgt gatgaaatcc gtcaaatcga
     2581 acccggcctg tatttgggaa aagtttattg ggagaaaaag cgattaattg attttgccct
     2641 gaaatttaac tcctagtccg ataagccgaa gttgtgactc taaacaccgc gtttagggat
     2701 cgtttccgcg gcggccatca cgatcgagcc atcattctcc acgaaaattc ccagctattt
     2761 cgatctcgag ccaatcttca atcgctacac gtcaaacgtc ggcaataact tttgatgatc
     2821 gtgacaatgc cagcgcaccg agtaaccatt gcggggggag aacgtctatg gcgcagcccg
     2881 aattagcgtc gccgggggaa cgtattctga agcgcatcgg ttggatcact gtcgtcgcgc
     2941 tggtgttgat tttgaccgca ggagcgttgt tgctgggacg tttcggcgcc gataccccgg
     3001 tggcttacaa ggacgacctc gagcatttca agtatggctc tctcggtagc gagcatgagt
     3061 tcggcgtgcc ctattggatt tggcgcgcct tacccgagct gttcgaagac aagctaccgc
     3121 aacctggccg cggctgggaa tcggtcggat ttgttttcga aaagggcaaa aatcttccgg
     3181 tcggcatgtc ccagcgccgt tatctcgggt ttgacgtggt ctggctcaac tgcgcgtttt
     3241 gtcacgcggg aaccgtgcgc gaaagcgccg agagcgagcc gaaagtctat tccgctatgc
     3301 cggctaacac gtttgatttc cgcgccttca tgaggtttct gttcgcgacg ggagaggaca
     3361 ggcgattcac gccgagggac atcctgcggc agatcaggat catccggcaa cgcgaagggc
     3421 tggatgattt accattgatc gatcggctgg tgttgcgttt ctacggcatc tatttcatgc
     3481 gcgagcgaat cctgactttg cgcgatcggc tggatttcat caaagagcaa cctgagtggg
     3541 gcccggggcg agtcgacacc ttcaacccgc tgaaggctta ttttaatttt cctcctgatc
     3601 agctttccga ggaggaacgg attggcacta cggattttcc gtcgatctgg aatcaaggac
     3661 aacgggaaag tctcaagatg aacctgcatt gggacggcaa caacgtatcc ttggaggagc
     3721 gtaatcgcag cgccgcaatg ggcaccggca ttacgccgcc taccggggat cggaccagcc
     3781 tgaagcgggt tgcagattgg gtgcgcaatc tcgccgcgcc ggattatccg tttgggatca
     3841 acaaagatct tgccgcccaa ggcgcgccga tctaccaaga gtactgcgcg aaatgccacg
     3901 gcgccgatgg caaggatttt cgcggcgagg acgtaggcaa ggtcgtgccg attgccgata
     3961 tcggcaccga ccgccgccgt ctcgactcct acacctacga cgttgccgtt aatcaaaata
     4021 tgattttcgc gggctatggc gaagaacgct tcagccactt ccggaaaact ttcggttacg
     4081 ctaacagccc gctcgacggg ctttggctgc gggcgcccta tttgcacaac ggctcagtgc
     4141 cgactctgcg tgacttgctg gagcccagcg acaaacggcc aaagtctttt taccgcggct
     4201 acgatgtcta cgatcggaag aaggtcggtt ttgttggcga cgtcagcgaa gagaaaggcc
     4261 ggaaatattt tcgctacgat acggcagagc ctgggaattc gaatcaaggc catcaaggaa
     4321 aacggtacgg caccgagcta ccggccgcag agaaagacgc tttgatcgag tacctgaaga
     4381 ctttttaatc gctggcgcgc tcctgtgtgg ccctggaggt tcgatggttg caggacaaaa
     4441 aaaatcctgg tggaaaactt gtctggtgct ggtgctcctg ttcgttgctg tggtcggcat
     4501 tatcggctgg tataaattcc tgcgtgagga gccgcaacct agctgggtga ccgcgacacc
     4561 cgatatgcgt tttaaatatg gctcgattgg cgccgagcac gatgccggca taccgtattg
     4621 gatttttttc gtactgccgc gcatgtttcc ggaaaagttg cccgggcctg gcggttacgc
     4681 ttccttagga gtcgcatggg agcagggtca agagctgccc atcggcttca ccaagaaagt
     4741 gatcggcttt ccgcgcgtgg ccaataactg cgcctcgtgc cacaccgcca gctatcgaaa
     4801 gagcgccgac caaaatcctg tgtttgtgcc ggcagggccc aatcacacgc tcaatctcca
     4861 ggcctttttc cgcttcatcg tagactgcgc ccgggatccg cgtttcaacg ccgatgagat
     4921 catgagcgaa atcgctctgg tttacgactt gccgctgttg gatcggctga tttatcgcta
     4981 tgcgctgatt ccgatcacca agaagcgtct gctcgaacgc gaagctcaat ttaagtgggt
     5041 gtatcacaag gacttcccgt tgtggggccg ggggcgagat gacgccatga atctcaccaa
     5101 atactttcta ctcaactggc caatggacga taccattggt cccactgaca tgccgtcggt
     5161 gtggaatttg aaaaagtacc agccggaaaa gggaatgctg atgaaccttg ccggcgacag
     5221 ccatgacgcg cgttccgtga tcattgattc ggccttgggg ctgctcaatg ccgagcccca
     5281 tgatcgcgac gatttcatag ggcagattga ctggctggtg aaatatttga gcgagctgca
     5341 ggcgccaaag tatccgtttg cgatcaaagc ggatcaagcc caaaccggca aggccgtttt
     5401 cgacagtaat tgcgccatgt gtcatgcgag cgagcgcacc ggcacgcggg ttccggtcga
     5461 agaggtggga actgatcgag agcggctaaa aacctggagc aagcaggcag ccattgagtc
     5521 caacaaagtt gtgcgcaact tcggtatcga acgtcgcggt ctggttgaaa atgaaccgag
     5581 tggatatata gcggcattct tggatggcat ctggctgcgc tcgccgtatc tgcacaatgg
     5641 ttcggtgccg agtctgcgcg atttgctcaa accggccgcc cagcgtccaa aggtttttta
     5701 tcgaggctat gacgtttacg atccggaaaa tgtgggcttc gtcacccaag gcgcggaggc
     5761 gcaacgggtc ggcaccaaat acgatgtcga cgagcgcgcc agcggcaatc agggccatga
     5821 atacggcgtc aacttgtcgc ccgcggaaaa agacgcgttg atcgaatatt tgaagagcct
     5881 gtgaccatca gcaatgaaaa gatctatagc cgaaaagctg gccgattctt gagaatggca
     5941 gcgttgtggt tggggagaag taaaacaatg gaaaactgcc gtctgaacat cattgcgata
     6001 gtgatactcg tgacgctcgc cgctgccgcc tgtggcaaag gatcgcccga acccggctcg
     6061 gtcaaggacg aggcgttaac cgtcgggcgc actgcggaaa gcttgccggc agccgatgag
     6121 gattatttta aagacatgga tggcggggtg gagctcacgg tcaatgaggt gaagggacgc
     6181 aacaactgga tcgtttggag cggcggcaac gaccggtttt gggatcacct cgtcagcaag
     6241 agcttcggcg ccgtcgattt cctaaagatt ctttcatctc atcctaacat caagcatttg
     6301 agccgggata cgcgctggca ttaccttggc ctggtaaacg agccctgctt cgagaagcca
     6361 cagaccgcca acaaagatcg gttcggcctg tggctggata cacgcagcaa agattgcccg
     6421 ccggacccgt tcgaaaacga aaagaaatat cccgggatcc agattggcgc gcgcggtaag
     6481 aacattccgg tgggatcgtt gtacggctac gcgtcgggaa ttgtcggcct gcgcctcttt
     6541 ccgaatccgg acttcgacga tgcggcgcaa aagaaatggg atgcggaaaa attttataac
     6601 gaccctagct attacaacga taagaatctg attcgtccct atagggtcgg catgtcctgt
     6661 ggcttttgcc acatcgggcc gagtccggtt aaaccgccag ccgatccgga aaatcccaag
     6721 tgggaaaatc tgaattcgaa tcccggcacg caatatttct gggtagaccg aattctcttc
     6781 tggcaaaagg atgacgctaa tttcatccag caactgctcc atacgtcttt accgggcact
     6841 ttggatactt cgtttatttc cagcgattac atcaataacc cgcgcacgat gaatgccgtc
     6901 tacagcgtcg cggcacgatt gaaagccgcc gaacgctggg gagaggaaaa actcgccggc
     6961 gggggagtca aaaacaagca attccaagac ttcaagcaaa ccgcggcttt ggcgcagtac
     7021 cacaaaccac ccgacaccgt gtggacgccg cgtatcctca aagacggcgc ggattccgtc
     7081 ggcgtgctcg gcgcgctgaa ccgggtgttc atcaacattg ggctgttcag cgaagagtgg
     7141 ctgctgcatt tcaatgctct ggtgggcggc aagaaaatta cgccgatcaa aatcgaggac
     7201 gccgagaaaa attcgagcta ctggaacgcc accgttgccc agactccgga cgtcgcatta
     7261 ttcttcctca gaacggcgcg gccggacctg ctcaagaatg ctccaggggg cgagcaatat
     7321 ctgaccaagg atcaagtcca gttggctcgc ggcaaaacgg tgttcgcgga gacctgcgcg
     7381 cgctgtcatt cgagcaagat tcccgcagcg ccggctggtg tggatgaacg gaactgggat
     7441 caatactggc agtggaccaa gactgacgat ttcaagaaaa agatgactga gatggtgatg
     7501 gcggatgatt ttctcgtcga caattatttg tccaccgacc gccgcattcc ggttaccttg
     7561 ctccagacca atgcgtgcag cccgctcgcg accaatggcc ttggtggcaa catctgggac
     7621 aattacacat cgcaaaccta taaatatctg ccatcggtag gtaagattac cgtgcacaat
     7681 cccatcgatg gcagcccgca ggaatacgag atgcccggca acggccgcgg ctatactcgt
     7741 gtgccgtcgc tgatcagctt gtggtcaacc gcgccttttc tgctcaacaa ttcagtcggc
     7801 aggttcaatc cttcgccatc cgtcgaagcg cgcatcgaat cgttcaatga ttcgatagaa
     7861 aaaatgttgt ggccggagaa gcgtgacaaa gatttgattc ttggtgacaa ggtgccgggc
     7921 ttcatctacc gcacgaccgc gccgagctat atcaagcttg cccccggatt cctacctgcg
     7981 tctctgaacc atcttctcag ctggaatgac tggggggcgc gctggttgcc atggctgttt
     8041 agtgaagagg gggtgcagat cggccccatt ccaaagggca ctccggtaaa tctgcttacc
     8101 aatatcgatt tggaaagcag caaaatcgat ttggccaagc tgatactcag aatgaaggcc
     8161 gatcttaagg cggtcgaagg caagaacgaa caggaagcgg ccgaggtgtt taaaaacctc
     8221 gtgcccgatc tcttgcaggt cagcaagtgt ccggatttcg tcgtcaaccg gggccactac
     8281 ttcggcacga gcctgttgaa ggaagaaacg ccgctgagcg atgaagataa acgagcgttg
     8341 atcgaattct tgaggacgtt ctagcaatgt tctccggcca actccaataa gaatgcctgc
     8401 cgcgaatgcg ccatcgaatc catatgatta catcgtcgtc gggtccggcg ccggcggcgg
     8461 cacggtcgcg gcgcgattgg cggaagcagg atgcacggtc ctgcttctgg aagcgggcgg
     8521 cgatcctctc ggtatagccg gcacacgttt gccggaagac taccaagttc ctgcctttca
     8581 tcctttcgcg tcagagaatg ctgctatgcg gtgggatttt ttcgtgcgcc attacggcac
     8641 ggacgagcgc cagaagaagg acgcaaaatt taccgctgag agagacggcg tgctgtatcc
     8701 gcgggcgggc gcgctgggtg gttgcacgag ccataatgcg atgattctgg tctatccgca
     8761 taatcaagac tgggatgata tcgccgagct caccggcgat gcctcctggc gggcagagaa
     8821 catgcggcgg tacttcgagc gtctcgagga ctgccgccat cgacctccct atcgctggct
     8881 taagaagatc ctgggattaa atccgacgcg ccacggtttc gacggctggc tgacaaccga
     8941 gcggagagat cccagattgg tgcttggcga tcgggatatg atggaaatga tcaagcgttc
     9001 cgcggtccgg gcgttcaaag agatggatga tccgctgacc caagttagat ggtcgatcaa
     9061 atcgcagcat gatccgaatg actggcggtt ggtacaatcc aattcatttg gcgtacgcta
     9121 ttcacctctg accacgcgca atcaggcgcg catcggcact cgcgagcgca tcttggatgt
     9181 ggctaaaaga ttcccccata agctgcatgt cgagccccac gccctagtga cgcgggtgct
     9241 gttcgacgaa aataatcgcg ccttaggcgt cgagtatctc aaaggggagc gtctttaccg
     9301 cgcccatggc gatcccagca gtgacgcggg agaaaaatgc caggcgtttg cttcgcgcga
     9361 gacgattttg tgtggcgggg ctttcaatac gccgcaattg ctcatgctct ccggcatagg
     9421 cccaaaaaaa gatttgcaat ctcacggcat caaagtgcgg gtcgatcttc ccggcgtggg
     9481 caaaaatctt caggatcgtt atgaggtgag cgtcgtcaat cgcatgacgc aggactggaa
     9541 tgtattggca gcggcaaagt tcgataccag cgatgcccag tatcgagagt gggccgcgga
     9601 tcgtaagggt gtctatgcca ctgccggcgc tgcgctggtg gtggttaaaa aatcccagcc
     9661 gggtcgtacc gttccggatc tgttctgcct cggccttgtc ggcaaattca agggctattt
     9721 tccgggatat tcaagactta tctcagcgca ccacaactat ttgagttggg tggtgttgaa
     9781 agcgcatacc cgcaataacg ccggcacggt gagtttgcgc tcggccgatc ccagagatac
     9841 cccggaaatt aactttcgct atttcgacga gggtagcgat gcgaggggag atgatctcaa
     9901 aggcgttgtc gaaggcatca agttcgtgcg ccaaatgacc cgcgcgctta aatcagaaaa
     9961 gatggtggcc gaggaggaat taccgggcaa tcaggttcag tcggatgccg agctaagcga
    10021 ttacgcgcgc tataacgcct gggggcacca tgcttcctgc acctgctcca ttggggctat
    10081 agaaaaaggc ggggttttaa cctgcgattt caaagtgcac ggtaccacgg gattgcgagt
    10141 tgtcgatgct tcggtgtttc cacgcattcc aggcttcttt atcgtcagcg ccgtgtacat
    10201 gatcggtgaa aaagccgccg acgtgatcct ggcggataat cgctagctac agtagctcag
    10261 attccgcgga gcattgggag ctcccattgt cttgggattt ctgccaatcc ccgattccgc
    10321 ggtggaaatc ggcaatgtgt cgcgatcaag cggtgagcct ggaggagcag gccgctctgc
    10381 ggcatctcaa atctcaagtc aaatgacaga tgtgagatct gaaagctgag actccgagcg
    10441 taccgaggac tggggctggt tgatcgatgt atcacattga cgcggaatcc gagctaattg
    10501 cttttaaagg agaagtccgc ttctgcgccg cgccgcggct tccagtcgag cgagtaatat
    10561 tcgccgcgat cccgtgccca gggatcgaaa acattcacca ccggttcgag ttcttcgcgc
    10621 aattctggaa tcgttctctg aaggacgcgc tttaacggcg aaacttcgac cgtgtgtccg
    10681 ttggttttct tccgctcgaa gattttgccg ccaggcccgt tattgtttac ccagttgtag
    10741 ccgaggttgg tgtaaaacgc cggtcggaaa ctcgaggtta aaaaacgatc gctgaaaagt
    10801 cgccgagaag cgttcaggat gaatacctgg aattgtgttt ccgagatggc aaaaccgtgc
    10861 ggccgcgcga attcggcaag ccatcctacc atagcgtcga catcctctat gttatccacc
    10921 agggtgccgt ccggatggcc aaggcagtcg tcgatttcgc tgccgtcgtc atttttttgg
    10981 gccgtcgtga tgatcctcga agaatcacaa cgatgttgtc cgtagatctc acgaagtagc
    11041 cccgtcaact tttgctgctc tgtccattca ccggagttct ttgccagccg cgggtcgatg
    11101 aaatcgtcga acttggtcaa ttgtttaaga ccgtattggc gtcgatactc attgtaacgc
    11161 ggaatgccgc gttccctgtc gcggatcaga tctagagcgg ccacatctat ttgaccgctg
    11221 ccgcttttta ggcgaggcat agacaggctt tgtaaaaatc tcgggtggtt gcgcaacgtc
    11281 agttttccta gccgctgccg ccccaacgag agcgcccagt tggccggccc gtgctgatgc
    11341 atcgcctggg tcgctttgcc gcgcagagtt tcggaaagag caatcttcga tttgacgacg
    11401 ttgcgatcgc ggttcagctc gcgaacttcg atgaagtcgg ggagcatcgg atgcaaacgg
    11461 tacgcattga tgaactcctc cggaaaatta aacggagagc cgaagtggtt gacgccagca
    11521 ttgacgtcgg cttgggtatt gcccagaccg acgatacccg gtccagcggc gaaagccgcg
    11581 taccatccgg tggatttttt gccatcggcg gatcgggtaa accgctggat cacgcgttcg
    11641 agagcggcgt tgaccaatgg atgctcgtca aacaggcccg accaattggc attcatagcg
    11701 agatacaccg gctcgttgta aagtagctgg gtagtccact cgatggtgtg gatcttggca
    11761 atctccgccg caaccaccaa acgcgcgact tcgaaaagct cgttggctgc gacctccttg
    11821 ttgctgacca ccctaccggg ctgcgccggc cgccgcagtc cggagtccgc atccgggtct
    11881 gtagtcgttg ccaaacgaaa agcatcgacg aaaatattgt gctcgcgcgc gaatacgttg
    11941 tggaaaaaac ttagaccgat gttccagttg tccggaaatg caaccgcttc ctggccgctc
    12001 cactggggat tcatgggatc gccagaattc aatatcggca aatactgggt gttctctcgt
    12061 gcgcctgtcc tggccggatc gagtaggagc ttggccggat cgcgcgagtc gcgcttcacc
    12121 cgccgttgcg aagtgtcatc atagccatag atttgcgaag catcccacca tgcggtgact
    12181 ttgttggctg tggtcctatg cgcgcgcgtc atgtaggagt tgttgccgaa gctgaaggtc
    12241 ggtgccggga tgtcctccgc gatcaaactt ttctcgacgc gatcggccgc acggcagcca
    12301 accggttggg ttttcgtcga gctcgaacta ctttgatcgt ttgacgcttg atgattatcg
    12361 cagcccatcc ccatcattgc cgattggttt tgcccctcgt ctagatgaga aaaccaatcg
    12421 tgggtcatga actgaatcca gaatccagcg agcagattca gatgcgacgc tttttgatag
    12481 tcgcaacctg cctgatcggg ctgttccggc aatccgtggc tatggcactt gccatccaga
    12541 gacccttttc gggtgaataa tttttggctg atcagctgtg ggtcgggttt taacaaaccg
    12601 attcgaccgc cgtggcgatt ggccgcgatc gggtcgacgg ccaactccgg aaacgtgctc
    12661 tcgaagtcaa cgttgcgcgc gaaccgttgg ccgcttgagc ccatcagcgg attggtgatg
    12721 tcgttgcaaa ttccagtgat cgtgcgaaag cggatcagct caccggagca tagttgaggg
    12781 ccattgccgc ctacatctgc atacaacgga tgatcctttg gcaggcgcaa ttccggccac
    12841 atcctgctga catgacccga tgggctgtgt gactcgcgta tgtaggtttc gaatgtgccg
    12901 ttgttatcga atagattgaa cttcagcagc tcgagacgct ggtactcgag atcaagcagc
    12961 gcgccgttga tcccgcgtcc gtccgggctg aagaagccga gcttgccacc ccaacccgca
    13021 gtgcggcttt caggtccccc ggcacccgca tatcgttgcc agtcgatcca cggtgtgttg
    13081 cgccaaacca ccgctgattc gccgccgcgg cagcgcgcgc tgtcctcata gacttttcca
    13141 agaaagcgcg ccgagcgttg ctgcgccacc tcgcgcaaac cggccagaga ataggctatg
    13201 cagctaccga cctgagtttg tctggtcaga ggataggcgc cgactaagac cagcgcgagc
    13261 attaaacaaa ataagaaagt ccccttcatg gcacccggtc aaagctgtcg attttcgtcg
    13321 acgtcttctg ttctgatttt ctcagtatgc cggatcggca atcgaagagt caatttatcg
    13381 gagcgccgag cggctcggtg ataaggcttg atgcccaatt atttgccctc tatgctaaag
    13441 actgtatagc tcaggaacac gtcgaaacag cagataacca catcaacgat ccgacaaaat
    13501 tctcccgaca taatctactc aacatggctg ccattcccgc cgaattctac gccctggcca
    13561 acgcgtttct attcgccttc cataatatgc tgaccaaaaa ggcgatgcgc tactccaatc
    13621 ctgccactgg cgtaatcagt agcctgttga ttaacattgt atttctctgg agcgtgtcga
    13681 tcttgttcgt gccgctgtcg tctttaacca gcgcgtcgat tttgattttc gttgtcgtcg
    13741 gtttttttca acctggattg acccgcctgc taacctataa aggcatcgat gcgcttggga
    13801 tggcgatcac cgatccaatt cgggcgacca cgccgctgtt cagcgcgatg atggcgataa
    13861 tttttctcgg cgagcaaatc accttgccga ttatcgttgc cacattgatg attatcgccg
    13921 gcataacctt gttgtcttgg cgcagcggtt cagtgaaact cgcgggctcc gctgtctttc
    13981 tttggtaccc catcgctgcc tcggcgctgg caggcgcttc tcaggtggtg cgaaagttcg
    14041 gtttggcagc cgtcccgcat ccgttcctag cggcggcggt gaccgcaagt tcctccttcg
    14101 tggtttcgct gttgacgctt tggtatgtgg aaaagagcga ggaaacctgg aaaatgaacc
    14161 gacaatgctt ctggtggttt ctcgccgccg gcatcacgat cagcctcggc atggtgtgca
    14221 tctactatgc cttggatttg gggaaagtct cggtcgtaat tccgatcagc agcaccggtc
    14281 cgtttttttc gctgatcttc agctcgctct ttttacgcga cgtcgagcgt gtcacgggtc
    14341 ggatcgtcat cagtgccgcg atgatcgtcg gcggcgtgtt gctgctgaca atttggaagt
    14401 aattcgtttt tcagtcgttc gctctctctt gcagaagaat tgtggttgac gatcttatcc
    14461 ttcaactgcc gctacacacc gttttgtctg ccgagaaaaa gttcacgctt tatccggcat
    14521 gctgggactc gtaaaaacgg ctcgaaaccg ttatcggatg ggggatttgt agtagagcca
    14581 tttcgtggca tagatgaatg atagtttttg cgtcatggca gcggaatgct gctctgtaat
    14641 gtcgaggaag ttgcgcctaa tgtattaaca cgggccgaca ccaggaggtt gcaaacatga
    14701 tcaacgactc gtgtcatcct aacgctttta ctgactcaag gccaaagact ggtattgttg
    14761 cctgcctttg gcttctcatc attttaagtg ctgctatgaa taatttgccg gggttcgggc
    14821 tcgagagtgg cgcggctctc gcggcggatg cggcagacca gccgggcaca tatggctcgg
    14881 cgtttgggtt cggcaggcaa tcgttgggaa ttagcgccgg gcatggttta gcactcccgg
    14941 ttggtggtac cgataacccg gagatggaag atattcaatt tgtctatgtc actccgcgct
    15001 gggtatcggc atcacggatc ccttgggagg acgtagttgg taccggggaa acttcgagtt
    15061 aatgctcgaa gggaccttcc tttatatgtt cgaacccaaa agtggcatcg ccggaggtat
    15121 cgctcccgcg ctgcgctata atttcttaac cggcactcgg tttattccgt tcctccatgc
    15181 cggcgtcgga ttcatcgctt tggacgccga tctggccagg caatccgacg gcttgaattt
    15241 tatcctgcaa tcaggggtgg gtcttcacta ctttctaagc caaagcaccg cgttgaccgg
    15301 agagtggcgg tttcatcata tttcaaacgc aaatattcac gacagaaatg ccggtatcaa
    15361 cagcagtctg ttcctgctgg gccttacttt cttcctcaga tagaagctcg ctcgccgcgg
    15421 aaaacatgaa gcggtgcaag cgcaggtatg agtcgaagac cgcggtcaaa cgatcgttag
    15481 cgcaatcatt tccggctggt ttcgataccg gccagagcca aagcttctgt gagactagag
    15541 atcgccgggc cgcgccattc tctcgcttcg gtatgcaaca cactaaggga ttcttcggag
    15601 gatgatcgaa ttagcaccgg ctgcaaccct gttctagccg cggcggccaa ttcatgctcc
    15661 tctccatcgg caatatagag gcaggatcga ggtttgctgc cgagttgttc gcatgccaga
    15721 tgatagatcc tctcgtccgg tttcttcaaa cgagcgaggc aggagaaaat tgtcgcgtcg
    15781 atggcgcccg cgaaatcggt ttcaggccag agcagaggaa tctccaccga gcaattgctg
    15841 agcaggccga gtccgtatcc ccgccgcctg agttccttca gtgtggttat agcgtccgcc
    15901 ttcggccgca gtgcttcttt gatgtatttc atacggagcg caacggcatt tcgaatctgt
    15961 tccgggcgcg cctgcgcatt cattttatcg agcacgtatt gaatgctggc ctcgaccgtt
    16021 tcaaaagcgc cgatgaccct catctccgtg gttcgactcc aaagccggtt gaattcttcg
    16081 ttaggcacct ccaggacggc aaccatttcc cgatgcattt ggcgggcgga agacgggaaa
    16141 tgattgacca aggttccaaa caagtcgaaa atgacggttt cgaatttcat gagttaaaaa
    16201 tttccgaaac tgtcacgggt gcggtcacac aagtacggtt ttgctgcgct tgagcctcgg
    16261 tgatcgatac tccaatcgaa ctttcaacct cgaaccttag cgcacctggt tactcactcg
    16321 tacagcactc ttttgatttt tctcttcatg tcctcaggca gattgaagaa cgcgttcact
    16381 cgccgagtca gttcatcgcc ggagatcggc gcaacgccga gccgggcttt tttggcttca
    16441 gcgagaaatt ccggatcgcc caacgtcttg tcgaaggctg atttgattgc ggcgatccga
    16501 tctttgggca ctcccggcgg aagcatgaac gagcgctcga cgatgttgcc gccatggatt
    16561 accacatcga tcagctgttt gccttcatcg ttcttgccga aattaatcgc cagcggcact
    16621 ttgggcagat cggcatgcgc tttcggcgcc gcttgcagca ccacaacgcc atcgccggtc
    16681 tccatggctc tactccaggt cgacttaatc gaatcccatc cccagcagga accatcgacc
    16741 tcgccggctt ccattgccag tcgcacgggc gccgtgcctt tatatccgga gaccagttga
    16801 atcggaaagt tggtcgcggt gctgagaact cgcaacgtat tatcggtcag gttcccaggg
    16861 ccgacggcgc ccatcttcac tggccgcggc gaggcaagcc acttatccgt gctgacaatg
    16921 ccgcttgcct tggtgaaggc gcaggagaaa tgatccgcgg taggcgcgcc caggtaagta
    16981 aactttcgca tgtcgaattc gacaccgggt tgcccgagta tctggctgat agtctggctc
    17041 ccggtgaagt ggccgatcgt gaggccgtcc ggcttggctg ctttatagat gtgattggcc
    17101 gcgatcatca tgccggcgcc gggcatattg tcgacgatga tagtgggttg cccaggaatg
    17161 tgcttggaca tctgccgcgc gatcatacgc gcataggtat cgaaaccgcc gcctggaggg
    17221 ccgccgacga tgatgcgcat ggtcttgcct ttgaagaaag tatcttgcga ataagcagtc
    17281 gatacagcca agaacggcag caaaacgata gccagaagaa aacttgcaca gctgattttt
    17341 cgtttcattt acttgcctcg ctaggttttt gcccgtcgat ttacttcaag ctcgcaccct
    17401 ggctggcgaa ggcgcgttcg atgaagccgc tctcttcgag tcttcgggcg atccggccgt
    17461 caataacgtc ctcgactttg agatcgccta ctttaggcgt gcgtgttttc aaaagccgct
    17521 gcacattgcg cagcccttcc acagaaggga tcggcttgcg atcgacgcca cgcgtgagat
    17581 cgagataccc atcttccgcg ccggcggcgt cggttctcag gtggcgcatc aatgatttca
    17641 aaaccgccgg cttgttcttg ggcgccagcg aataggccaa cgcttcgatt tcggctttca
    17701 agtaattctc cgctagatcg cctttctgct gaagcagcgc gccaggaacc accatcgatt
    17761 gaccggcgag aagcttggtc atctcggtgt attcgtgcag gatcgtcatg cctttttgct
    17821 ggagtttgcg gctgaatgcg ccgtcgagaa ccgccgcgtc gcagagacca ttctcgaccg
    17881 cttgcgcctg gatgccctga tcgccgacca cctgcatttg gatctgatcg cgctgttgat
    17941 ccaggccgag gtgctcaagc cacaggagaa tgcccatcca tgtggtgccc ccgatgctgg
    18001 tcaccgcaaa acgtttgccg cgcaaatctt cagctctctt gatgttgggc cggacgacca
    18061 gatcataggt gttgcggctg gaaaagctgg cgacgaactt aaaatcgtgt cccgcggcga
    18121 ccgccgccag catggcgctg ccgccggtgc ggccgatgtg gatgtcgccc gatgccaggc
    18181 ctgccaccag ggtgggggcg cctcggatca gaatcggttg cgcatcgatg ccgtacttgg
    18241 caaagattcc ttgctcgtga gcgatccaca gcggcatcag gcgcgcggtg acggtggcat
    18301 agcctattac ggttttcgcc ggcgcggcca cagcggcggc aggcgtgttc caaccaataa
    18361 gaaccccgac cgaacaaaat atcgtggcgt acatgttgat aaggtagcgt ttcatcctta
    18421 ctccttatta ctgatcagga ttcaacttct ctcgaaaaag ctgtgacaat tcatccggag
    18481 agccctggcc gacgatcgac agcgtggcag ataaaattca tcgccgtgtg ttgctcatcc
    18541 ttagtacagc gattggtttg ccccgcggca cggctgtaca ttaaccattc atacacatat
    18601 ccacttaaaa gcgaaaactt actaatccta tatgggatgt cccgcgagcc attcctttgt
    18661 gcggcgagtt tggcccgttt tatacctgat ccgccgcccc cggatcaatc gatagcttcg
    18721 tctcgagtcc catgccattt ttcaggctag cagcgacatc cttgcggtct cgtcacgatg
    18781 cagactggcc gcggtcgcgc agagaaggat gatcgatcga aaagagaaac ggggatttgc
    18841 ttttatatag ggctgcggtc tcgggtcggt gccatacaag gcca
//
LOCUS       DASUVS010000242        10606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1631301, whole genome shotgun sequence.
ACCESSION   DASUVS010000242 DASUVS010000000
VERSION     DASUVS010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10606
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631301"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(450..1949)
                     /locus_tag="VF182_13290"
     CDS             complement(450..1949)
                     /locus_tag="VF182_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6438106.1"
                     /translation="MDLIDNITLGFQTAFTIANLFYCLTGVFLGTLIGVLPGLGPTAT
                     IAMLLPLTFSLPPISSLIMLAGIYYGSQYGGSTTSILVNLPGEAASVVTTLDGYQMAR
                     QGRAGVALATAAIGSFFAGTVATFLIAAFGPPLAAVALSFGPAEYFSLMTLGLVASIV
                     LAQGSLLHAFGMVVLGLLLGLIGTDVTSGVPRFTFDIPELADGIGFVTVAMGMFGLSE
                     IIRNLETEAQRTVAVAKVTTLMPTRQDFKRMIAPILRGTAIGSALGILPGSGSILGAF
                     AAYSIEKKVSRNRAQFGKGAIEGVAAPESANNAGAQTSFIPMLTLGIPSNPVMALMVG
                     AMIIQGIQPGPSVITEQPALFWGIVVSMWIGNLFLVILNLPLVGLWVRMIMVPYRLLY
                     PAILVFCAIGVFSLRNSQFDVYMMALFGVLGYIFSKLGCEPAPMLLAFILGPLMEEFL
                     RRAMLLSRGDPLVFIQRPISATLLGIAVLAMCAVLLPSFAKTREVALKE"
     gene            complement(1977..2429)
                     /locus_tag="VF182_13295"
     CDS             complement(1977..2429)
                     /locus_tag="VF182_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX6438107.1"
                     /translation="MIRSTRDFWTGAIYILIGLSAVLIARDYDMGSAVRMGPAYFPTI
                     LGIILMLVGAVALIRSFIVLGTPIGAFAIKGLLLVVFSVCLFGFIVRDAGLVIALPLM
                     VLISAFASSQFRWLPTIALAAGLTVFCALVFIKGLGVPLPVIGPWFGG"
     gene            complement(2560..3531)
                     /locus_tag="VF182_13300"
     CDS             complement(2560..3531)
                     /locus_tag="VF182_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugD"
                     /protein_id="HEX6438108.1"
                     /translation="MQKIFVFIISFLCVALASANAQDYPTKVVTMIVPFAAGGPTDTV
                     ARLLGGPMTKTLKQQIIVENVGGAGGTIAANRVAKAKPDGYTILIHHIGMSTAPALYR
                     KLAYNPMTDFEPIGLINEVPMTLVAKNDFPAKDLKDLIAYVKANKNKVNYANAGVGAA
                     SHLCGMLFMSAIETEVTTIPYSGTAPAMNDLLGGQVDFMCDQTTNTTSQIKAGKIKVY
                     GVTTKKRVPSLPNVPTMDEAGLKGFEVSIWHALYAPKGTPKPVIDTLTKALQAALKDP
                     TVKQRFGDLGTEPVSEDRATPEALRKHLKSEIDRWAPIIKKAGVYAD"
     gene            3842..4714
                     /locus_tag="VF182_13305"
     CDS             3842..4714
                     /locus_tag="VF182_13305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6438109.1"
                     /translation="MAEKIRELLRDYQQGKLSRREFMRQAIIATGSLAVANSLIASLL
                     PSAAVAAQVPPTDPEILTHNVQYDGKAGPIFAYLVRPLKAGKYPAVIVVHENAGLNDH
                     IRDVARRFAKEGYVALAPDYLSRHGGTEKANPKGGGLSNIRELAPWQNVAEDTQAGIQ
                     YLRVLPDVRGDRIGMVGFCWGGEVTFATVTQVSALRAAVVYYGRSPEPDRLQNIKTPI
                     MAHYGEKDPGITKGVPDTEAAMKKYNKTYDYKIYSGAQHAFNNDTNPERYNAAAAREA
                     WDRTQEFFKKHLKA"
     gene            4838..5797
                     /locus_tag="VF182_13310"
     CDS             4838..5797
                     /locus_tag="VF182_13310"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438110.1"
                     /translation="MKLLFLVSLLWSCTMGEISAAPLRLSYSVVGPTVAGIWMAHEYG
                     TFKKHGLDVQLVYVPSSGTNIQALLGGSLDMSAPGISGVVLAAARGAPVLAIGAMTNR
                     PPMTLYVHPDITRTEQLNGQTLGITRFNSTAHTVTTLILRKIGLEQTATMRPLGGQPE
                     IQAAFEQKQIAGMVTSVKPRAPARALLNAADLDIPFAMNVMAVTREFLQKNPDAVERA
                     MKAYIEAVAKMNNDKEATLKVLAKYFKRNDASFLDETYGIVTRFTEKIPRVDARNVAT
                     VLEFEPVKGADVETLTPKVIDNSIVEKIAKEGFIEKVFGKSLR"
     gene            5941..6552
                     /locus_tag="VF182_13315"
     CDS             5941..6552
                     /locus_tag="VF182_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438111.1"
                     /translation="MNFTSIALVVLIAVGVTDDPVAQERLKASYASIDATNAIWNIAI
                     ERGFYKKHGLDVDVVYIGSTTVTSAAILGQDVPIAISRIGSVSDVAVRELLKSLGLKR
                     IDDVALRQIGGAPERISAFSQGAVAGFINPPGSIHLVRQSRPAQSPDQHGRSGEAAGI
                     SMGLCGNHQELLAKRRDTVKRLVMALTEATHYFKSDKEGRKRS"
     gene            6609..6779
                     /locus_tag="VF182_13320"
     CDS             6609..6779
                     /locus_tag="VF182_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438112.1"
                     /translation="MKILERIPYVTREGMKAQLEQARSANPASKVTLDDVIDDTVVRE
                     IEKDGFIERLYR"
     gene            6850..7821
                     /locus_tag="VF182_13325"
     CDS             6850..7821
                     /locus_tag="VF182_13325"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438113.1"
                     /translation="MSRKLISQSILFGPLLFILSFPICAQAQELKKVKIGYPAFSLTF
                     LTFFVAKDAGIFKKHGLDVELIQLAGAVQTSALVAGEIDHLTGITGPLVAAARGLPFK
                     GILITHERPLFWLIANPEIRRAEDLIGKTIAVDRLATLQDIIARDLIKRKGVSPERVT
                     FVQTGSVSNSVQSLSQNSVAAALLSLPHNVVMMQRGYREIASATQFNMRSASGGIATR
                     EAKLKQDPAGVKAVIRALFESMDFNRKEKTWMVNYIQNKWKLSLKAADEAYRFWLEGL
                     TPDGKIPLKELQDIYDLAYSTQMIPTPVSVAKVMDYSLTDEVLKERK"
     gene            7898..8893
                     /locus_tag="VF182_13330"
     CDS             7898..8893
                     /locus_tag="VF182_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438114.1"
                     /translation="MAALQIDLACGDYDIVRPLRDGTIRAEGMEINFVTINKPPEVHW
                     RMGINEEFDAAEMSFGSFVAGKAHGEYPFIGIPAFVYRKFRHSCAYVNVKAGIVKPED
                     LKGKRVGVPEWQMTATVWLRGFLQDDYGVQPQDVQWLTGGLESSGRKEKVPLQLPPDI
                     RVEAISEGKNLSGMLVAGEIDALLTAQVPAPYVQRLPQVRRLFANPREIERDHFQRTG
                     IFPIMHVIVLREEIYKRHPWIAQSLYKALLEAKTHCIETFYKNDSIHAVLPWAGHYVD
                     ETRQLMGHDYWPYGVELNRKTLETFLRYSREQGLSNRNMEVDELFAKETLQIFHV"
     gene            complement(9368..9874)
                     /locus_tag="VF182_13335"
     CDS             complement(9368..9874)
                     /locus_tag="VF182_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438115.1"
                     /translation="MPFNTKSIRPISGSRPSCCVAGEELASSAGSSEWVKSDKKNRPG
                     FGGLADATESGESSGAVLPERAMLRTQRGKRRTNLAQLWLQFGTISISIHEDAFADRY
                     LSANPNSAQATARRCMARRSPCFAKKPAARNLVSLGFSQSFSFDSLLNLLLYFSVALL
                     IDCVPDFD"
     gene            complement(9980..10309)
                     /locus_tag="VF182_13340"
     CDS             complement(9980..10309)
                     /locus_tag="VF182_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438116.1"
                     /translation="MGIGNPSEYGELAPILNEKSQASTDYAGRADDCFVITMPPPEVI
                     AQWDGNDDCRELNRIVRDLRWSGMISDALVVLAITGVIYFLPSLETLRAAVERTAVSV
                     AEASRAD"
BASE COUNT         2696 a         2823 c         2766 g         2321 t
ORIGIN      
        1 taactatacc cccgatatcg tcgttatcga tccgcaccgc gcttgcttcg tcacccatct
       61 ggaccgcgca agccgcgata acagcggcga tgatgaccgc cagtacgctc aggcacatga
      121 tttgtttact cctcatccag cgctcctttc ggttttcacg tcagcataaa tgcgaatcca
      181 gcagatctgc taagcctttg aatcacataa cgattgtggt cggagattat cgaagaacga
      241 actctcgtgt caactgaatt gaactcatcg atcgtgctat cgtggcaaat ctcgccgatt
      301 aggatgactg gagtatcacg ctccgccacg acgttggacg tttctgggtg aagcagataa
      361 gccgcagccc ggatgggaga ggttcttatt actggagcgt agagcaacta ggtcacgacg
      421 cgtaaggtcc cctggctaga actgacgcat cattccttga gcgctacttc cctggtcttt
      481 gcaaaagaag gcagtagtac ggcgcacatc gccagcacag ctatccccaa caatgtcgcg
      541 ctgatcggac gttgtataaa caccaatgga tcgccgcggg agagcaacat ggcgcgtcgg
      601 agaaactctt ccatcagagg gcctaggata aaagccagca gcatcggcgc gggctcacaa
      661 ccgagcttag aaaagatgta accaagaacg ccaaacaggg ccatcatgta aacatcgaac
      721 tgggagtttc tcaaactgaa gacgccaata gcgcagaaaa cgagaatggc tggatagagt
      781 aggcgatagg gcaccatgat cattcgcacc cagagcccga ccaatgggag attgaggatt
      841 actagaaaga gattgccgat ccacatcgag acgacaattc cccagaacag ggccggctgc
      901 tccgtgatca ccgaggggcc gggctgaatg ccttgaataa tcatcgctcc aaccatcaga
      961 gccatcactg gattagaagg aattcccaag gtcagcatcg ggataaacga agtctgcgcg
     1021 ccggcgttgt tcgccgactc aggcgcggca acgccctcga tggcgccttt accgaactga
     1081 gcgcggttcc ttgaaacttt cttttcgatt gaatacgcgg caaacgcccc aagaatcgaa
     1141 ccgctgcccg gcaatattcc caaagcggaa ccgatcgccg tgccgcgcaa aatcggcgcg
     1201 atcattcgtt tgaagtcttg ccgcgtcggc atcaaagtgg tgaccttcgc caccgccact
     1261 gtccgttggg cctccgtctc tagattgcgg ataatctcac tgagaccgaa cattcccatg
     1321 gcgacggtca caaagccgat cccgtcagcg agctccggga tatcgaatgt gaaccgcggc
     1381 actcctgaag tcacatcggt gcctatgagt cccagaagaa gtccgagcac caccataccg
     1441 aaagcatgca gcagtgatcc ttgagcgagc acgatggacg caaccagccc caaggtcatc
     1501 aacgaaaagt attctgccgg accaaagctc agcgcaaccg cggcaagcgg cggtccgaac
     1561 gcggcgatca gaaaagtcgc cacggttccg gcgaaaaacg agccgatggc cgcggttgct
     1621 agcgcaactc ccgcccgtcc ttgcctggcc atctggtagc cgtccaaagt ggtcactacc
     1681 gatgctgcct cgcccgggag attaactaat atcgatgttg tcgaaccgcc gtactgggag
     1741 ccgtaataga taccggcaag catgatcagc gacgaaatcg gcggcaacga aaacgtgaga
     1801 ggaagaagca tggcaatggt ggcagtaggt ccgagaccgg gcaacacgcc gatcagggtt
     1861 ccgagaaaaa caccggtcaa gcaataaaag agattggcga tggtaaaggc ggtttgaaat
     1921 ccgagagtga tattgtcaat gaggtccata gggatacctg tgtttagcga tccacatcac
     1981 cccccgaacc atggtccgat gactggcaga ggcacaccca gccccttgat gaacacgaga
     2041 gcgcaaaaca ccgtgagtcc ggccgctaaa gcaatcgtcg gcagccagcg aaattgactg
     2101 ctggcaaagg cgctaattaa aaccatgagc ggcaacgcga taaccaaccc ggcatcgcga
     2161 acgataaagc cgaataagca aaccgaaaaa acgaccagca agagtccctt aatggcgaaa
     2221 gcgccgatcg gggttcccag cacgataaag gagcgaatca gggcaaccgc tccgacgagc
     2281 atcaagataa ttccaaggat ggtagggaaa taagccgggc ccattcgcac cgcgctaccc
     2341 atatcatagt cgcgcgcaat caggaccgcg ctcaaaccga tgagaatata aatcgcgcca
     2401 gtccagaaat ctctggtgct gcggatcatg agaaatcgcc gggttgtcgc aaaaaacctc
     2461 ctagtgcgca atgatcaagc cgccttctac aacaatatgt caagaaaaaa aaggccgggc
     2521 atatgcccgg ccttttttcg ctgttgtaat tagatcccgc tagtctgcgt acacgccggc
     2581 tttcttgata atcggtgccc atctgtcaat ttcggattta aggtgtttgc gcaatgcttc
     2641 gggcgtggcg cgatcctcgc tgactggttc cgtccccaaa tcaccgaagc gctgtttaac
     2701 cgtcgggtct tttagcgcgg cctggagagc tttggtcaag gtatcgataa ctggtttggg
     2761 cgtgcccttg ggggcataga gtgcatgcca gatcgacacc tcgaaaccct ttaacccagc
     2821 ttcatccatg gtcggcacat tgggcagcga aggcacgcgc tttttggttg tcacaccata
     2881 aaccttgatc tttcccgctt tgatctggct ggtggtgtta gtggtttggt cgcacataaa
     2941 atccacctgg ccgccaagaa gatcattcat cgcaggggca gtaccgctgt aaggaatcgt
     3001 cgtcacttcc gtctcgatcg cgctcataaa cagcataccg cagaggtggg aggcggcacc
     3061 cacgccagca ttggcgtaat ttactttgtt cttattagct ttaacgtaag cgattaggtc
     3121 tttgaggtct ttggcaggaa aatcattttt cgccactaac gtcatcggga cttcgttgat
     3181 gaggccgatt ggttcgaaat cagtcatcgg attgtaagcc agcttgcggt agagtgccgg
     3241 cgcggtcgac atgccgatgt gatgtatcaa aatggtgtag ccatcgggct ttgccttggc
     3301 taccctgttg gcggcaatcg ttccaccggc gccaccgacg ttctcgacga taatttgctg
     3361 cttcagggtc ttggtcattg gcccgcccag cagcctcgcc acagtatctg tcggtccacc
     3421 ggcggcaaac gggacaatca tggtaacaac cttggttgga taatcttgcg catttgccga
     3481 agctagcgcg acacaaagaa agctgatgat aaacacaaag attttctgca tcatttcgct
     3541 cctccttttg ttccagtcaa tcgtaggtcc atccgatagg ccaagaaaca ctggttccat
     3601 gataccaaat tgtcagacct ttgcatgcaa ggcattgtct ctttgttaga cgactgacag
     3661 acctaaacca tcgggtcaac ggtttttggg gcggtacggc atggaaccaa ttcggccctg
     3721 ggagcgatcc aggacacagt gcaaacaaac aaggatacac cttgacctta ctgttccgat
     3781 caatatatag gttgcacgct ggcccgaatg aatagttcgt tgaaaaatca aaggagacct
     3841 tatggccgag aagataagag aactgcttcg tgattatcag cagggtaaat tgtcccgccg
     3901 agaattcatg cgccaagcaa ttatcgccac cgggagcctg gctgtagcaa acagcttgat
     3961 cgccagcctt ctcccttccg ctgcagttgc agcccaagtg ccgcctaccg accccgagat
     4021 tctcacccac aacgttcaat acgacggcaa agccgggccg atcttcgcct atcttgtccg
     4081 gccgcttaag gccggtaaat acccggcagt catagtcgtt catgaaaacg ccggcctcaa
     4141 cgatcacatt cgcgatgtgg ctcgccgctt cgccaaggag ggctatgtag ctttagctcc
     4201 cgactacctt tcgcgtcacg gcggcaccga aaaagctaat ccgaagggcg gcggcttgtc
     4261 caatattcgt gagctggctc cctggcaaaa tgtcgccgaa gatactcagg cgggcattca
     4321 gtacctgcgc gttcttcccg atgtccgcgg cgatcgaatc ggcatggtag ggttttgttg
     4381 gggcggcgaa gtgacgtttg cgacggttac tcaagtttcg gctctaagag ccgcggtcgt
     4441 ttactatggc cgaagtccgg agccggatcg tctgcaaaac atcaaaaccc cgatcatggc
     4501 gcactacggt gaaaaagatc ccggcatcac caaaggtgtt ccagatactg aagcagcgat
     4561 gaaaaaatac aataagacat acgactacaa gatttattcc ggagcacagc atgcgtttaa
     4621 taatgacact aatccggagc gctacaacgc agcagcggcg cgcgaagcat gggaccgaac
     4681 ccaagaattt tttaagaaac atctcaaggc ctaagccttc tctacgaaat tagatgcggg
     4741 tggacccgtc gacgaaacgg gcccgctcgc gcgaaacctt tctcttcgaa agattctctg
     4801 accggtattt tcttactttg ggaagaatta aagcgccatg aaactgctct ttttggtttc
     4861 actgctctgg tcttgcacaa tgggcgagat ttcggcggcg cctctgcggt tgtcgtattc
     4921 cgtggtcggt ccgacggtcg caggtatctg gatggcacat gagtacggca ctttcaaaaa
     4981 acacggctta gacgttcagc tcgtttatgt cccttcgagt ggcacgaata tccaggcgct
     5041 tctcggcggc agccttgaca tgagcgctcc cggaatcagc ggcgtcgttc tggcagcagc
     5101 ccgcggcgcg cccgtgctgg caatcggcgc catgaccaat cgcccgccga tgaccttgta
     5161 cgttcatcct gacatcaccc gaaccgagca attaaacggt cagacccttg gcatcaccag
     5221 gtttaattcc acggctcaca cagtaacgac cttgattctc cgtaaaatag gattggaaca
     5281 gaccgcgacc atgcgtccgc ttggcggcca gccggagatc caggcggcat tcgaacaaaa
     5341 gcagattgcc ggtatggtga cctcggtcaa gccaagagcg ccggcgcgcg ctctgctgaa
     5401 cgcggcggac cttgatattc ccttcgccat gaacgtcatg gcagtgacgc gggaattcct
     5461 acagaagaat cccgatgcag tggaacgcgc catgaaggct tacatcgaag ccgtggcgaa
     5521 gatgaataat gacaaagagg caactctcaa agtgctggcg aaatatttca agcgcaatga
     5581 tgccagcttt ctcgatgaaa cctacggtat tgtcactcgc tttactgaga agataccgcg
     5641 ggtcgatgcg cgaaatgtcg ctacggtcct ggagttcgag ccggtcaaag gcgccgacgt
     5701 agagacgctg acccccaaag tgatcgacaa cagtatcgtc gagaaaatcg ctaaagaggg
     5761 atttatcgaa aaggttttcg gaaaatcgct gcgctaactc gagctctctt aggaaaaaca
     5821 acaaatttca gaattccaga ctcataaatt tggttgctct ttcagcgccc ttgtgaagtc
     5881 cgaacgcaga actctgattg tctccatcgg gcagcagcag gcgaaaggta cgtcggcgca
     5941 atgaatttta cctccatagc tctggtagtt cttattgcag tcggtgtgac cgacgatccc
     6001 gttgctcaag agagacttaa ggcctcctac gcatcgattg acgccaccaa tgcgatctgg
     6061 aacattgcta tagaacgagg cttctataaa aagcatggcc ttgacgtcga tgtcgtttac
     6121 atcggcagca ctacagttac atcggcggcc attctcggcc aggatgtgcc gatcgcaata
     6181 agccggatag gcagcgtctc agatgtagcc gtgcgcgagc tgctgaaaag tttgggactg
     6241 aagcggatcg acgatgttgc tttgagacaa atcggtgggg cgccggaacg aatttccgcg
     6301 tttagccaag gcgcagtggc ggggtttatc aatccgcctg gaagcattca cctggttcgg
     6361 caaagccgtc ccgcccaaag tcctgatcag catggccgat ctggagaagc cgccggcatt
     6421 tccatgggcc tgtgcggcaa ccaccaagag cttctggcga aaaggcgcga cacggtaaag
     6481 cgcttggtca tggctttgac tgaagcgacg cactacttca agagcgacaa agaggggcgc
     6541 aaaagatcat agccaagtac catgcttcgg ctaataaggc gtaccttgaa gatgcttatc
     6601 gtacaacagt gaaaattctg gagcgaatac cctatgtaac gcgcgaggga atgaaagcgc
     6661 aactggaaca ggctcgcagc gccaatccag ccagcaaggt tacgctcgat gatgtgattg
     6721 atgatactgt cgtccgtgaa atagagaaag atgggttcat cgagcgcctc tatcggtgaa
     6781 caatctagtt tcagcaaaag tggcgtgagg cttatcggcc gcttaggcac caaacgaact
     6841 ctaaaggcaa tgtcacggaa attaatttcg caatctattc tgttcggacc tttgctgttt
     6901 atcctgtcat ttccgatttg cgctcaggcg caggaactta agaaagttaa aatcggctac
     6961 cctgctttca gccttacctt tctgactttc ttcgtagcca aggacgccgg catcttcaaa
     7021 aagcatggac tcgatgtcga gttgatccaa ctggcgggag cggttcaaac atccgccttg
     7081 gtggcaggcg aaatcgacca tctcaccggt atcaccgggc cgctggtcgc cgccgcgcgg
     7141 ggcctgcctt ttaaagggat cttgatcacg catgaacgac cgctcttttg gctgattgcc
     7201 aacccggaga ttcggcgcgc cgaagatttg atcggcaaaa ctatcgccgt cgatcgtctt
     7261 gcgactctgc aagacatcat tgcgagagat ctgatcaaaa gaaaaggtgt gagtcctgag
     7321 cgagtcacct ttgtccaaac cgggtcggtt tccaatagcg ttcaatcgct gagccagaac
     7381 tccgtggccg ccgccctact ctctcttccg cacaacgtcg tcatgatgca gcggggatat
     7441 cgcgagatag cttccgccac gcagttcaat atgcgctccg cttccggcgg aatagcaacc
     7501 cgcgaagcaa agctgaaaca agatcccgcc ggcgtcaaag cagtaattcg cgctctattc
     7561 gaatcgatgg atttcaatcg caaagagaaa acgtggatgg tgaattacat ccagaataag
     7621 tggaaattat ctcttaaagc cgccgatgaa gcgtatcgat tttggctgga gggcctcacg
     7681 cccgacggca agattccatt gaaggagtta caggatatct atgaccttgc ttactccaca
     7741 cagatgatcc caacgccagt gtcagtagcc aaagtcatgg attattcgct gactgacgaa
     7801 gtcttgaagg aaagaaaata gccttccatg agacattttt catcgcctac gctttgaagc
     7861 tttcttggtt gatgcaacgc tggttcagga gtttaacatg gccgctttac agatcgactt
     7921 agcttgcggt gattatgaca tcgtgcggcc gctgcgcgac ggcaccatta gagcggaagg
     7981 aatggaaatc aatttcgtta cgatcaataa accgcctgag gttcattggc gcatggggat
     8041 caatgaagaa ttcgatgccg ccgagatgtc gtttggcagt tttgtcgccg gcaaagcgca
     8101 cggcgagtat ccgtttattg gcattcccgc tttcgtctat cgcaagtttc gacactcatg
     8161 cgcttacgtc aacgtcaaag ccgggattgt taagcctgag gacttgaaag gaaagcgcgt
     8221 cggtgtaccc gagtggcaga tgaccgccac tgtttggttg cggggctttt tgcaagacga
     8281 ttatggcgtc caaccgcaag acgtacaatg gctgacagga ggacttgaat cctcgggccg
     8341 caaagagaag gtgccattgc agttgccgcc ggacataaga gttgaagcca tctccgaagg
     8401 caagaatctt tccggcatgc tggtagcggg cgagatcgat gccctactga cagcccaggt
     8461 gcctgcgccg tatgtgcagc gactccctca ggtacggcga ctcttcgcca acccgcgtga
     8521 gatcgaacga gatcattttc agcgcacagg aatattcccg atcatgcacg tgatagtcct
     8581 tcgcgaagag atctataaac gccatccctg gatcgcgcag agcctctata aggcgctgct
     8641 cgaggccaag acgcactgca tcgaaacttt ttacaagaat gactcgattc atgctgtgct
     8701 gccttgggcc ggccattacg tcgacgaaac cagacaatta atggggcacg attactggcc
     8761 gtacggtgtc gaactcaacc gcaagacctt ggaaactttt ctccggtact cccgcgaaca
     8821 aggcttgagc aaccggaata tggaggtgga tgaactcttt gcgaaagaga cgctgcagat
     8881 ctttcatgta taaaacggat gatcgcatcg cttgagatag ccgcgcgtca atcgtttatt
     8941 ctcctcaccg tttgctgcta agtcacagag cggtcggtcg ctgtgcaacg ctcctgcata
     9001 ttacctagct attcatgttc aacgtctaga ccgttgtcgg ttagattaga cggaagattt
     9061 gactcgccat actaaaggat tgtactggca agcgctattt gacgttcatc gattcacaaa
     9121 gctgccgcta agtccacacg agtcacatga taactgcggc agtcgccgca atatgcggca
     9181 aataaccggc attgcttcgg ccgcctgcac ggaaatcagt cacaatctag agttggcagt
     9241 tctgaccgat aaggcttggc gtccttcaaa gaagtcgtcg ttgccggagc tatccaggga
     9301 atagctaggc aaaaagcacg acaaagttga cgtgcagtgg atttcgcagt cccggcacac
     9361 ggcctgctca atcgaagtca ggtacgcaat caatcagcag agcaaccgaa aaatacaaaa
     9421 gcaagttaag gagagaatca aatgaaaacg actgagaaaa acctagagag accaagtttc
     9481 tggcagctgg ttttttcgca aagcaaggtg aacggcgcgc catacaccgg cgagcagttg
     9541 cctgggcgga attgggattg gccgaaagat acctatctgc aaaggcgtct tcatgaatcg
     9601 aaattgaaat ggtaccaaac tgcaaccaga gctgagctaa attagttcgc cgcttcccgc
     9661 gctgcgtcct tagcatagct cgttcgggaa gaacggcacc actggactca cccgattcag
     9721 tggcatcggc caggccccca aagcctggcc gatttttttt gtccgatttt acccattcgc
     9781 ttgaaccggc cgaactggca agctcttctc cagcaacaca acaagaaggt cgcgaaccag
     9841 aaatcggtcg tatggatttg gtattgaatg gcaaaaagct tttacccgaa acctcggtcg
     9901 agcaagatat cgggccaagg agcaggcgga ccggagatgg ttgggactca agcgaatgcg
     9961 gggaatagtc agcttgagcc taatcggctc gtgatgcctc ggcgacggaa acggcggttc
    10021 tttccacagc agctcgcaga gtttccaggc ttggaagaaa ataaatcacc ccggtgattg
    10081 ccagcacaac caatgcgtca ctgatcatgc cgctccagcg aagatcacgt actatacgat
    10141 tcaactcacg acaatcgtca ttcccatccc actgagcaat cacttcgggc ggcggcatcg
    10201 ttatgacgaa acaatcatcg gcgcgacctg catagtcggt cgaagcctgt gacttctcat
    10261 tgaggattgg tgctagctca ccgtattctg aaggattgcc tattcccatc tcatcccttc
    10321 ctatgggata cgtccaccca accgcgctga aaattggggc gaccgagttg ttgtctttgt
    10381 ggttttccgc atctgtttag gcatcgctgc atccgcaggt tcaaatctgc aattcgttaa
    10441 ttgctcgata gcaacggttg tgccgattaa aggcagcgat gaggacgtac tttcgggtat
    10501 caaaccgaag ctttatcaag gatttgtaat tctaagactt agaaggtccg ttaccaaatc
    10561 aaacacttta tcgttcactc gatcattttc gtacgtcacc taaacg
//
LOCUS       DASUVS010000243        10829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_100564, whole genome shotgun sequence.
ACCESSION   DASUVS010000243 DASUVS010000000
VERSION     DASUVS010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10829
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100564"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VF182_13345"
     CDS             complement(<1..691)
                     /locus_tag="VF182_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438117.1"
                     /translation="MVYDDRSVKPMSASLMRARIATKILSGLYEDVPTLLTSAIRLMV
                     ELPGGSAHSGPPYPFTIGVSPAWCSGLNGAKLVASGKLDLVWLNPSIIPSMAVRGVGP
                     FRRKYPLRALAVFPSWDRLVIAVSPQLGVRTMEELIAKKPKMNVSIAVNDCVDFAIKY
                     LLKCHGISQKDFIDWGGTVDEVVRPSNPHRREGIVSGTLDMVIDEGMDSWGDVAVEHG
                     MRFLSFSEPVLK"
     gene            1031..2182
                     /locus_tag="VF182_13350"
     CDS             1031..2182
                     /locus_tag="VF182_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX6438118.1"
                     /translation="MYYPDLSGFGVDNDRLASQRLSRLQQAMQQHNLAALMLTDWLNI
                     RYATNSVFMMNLRATAVQRFVLVPATGAPLICQRQLSSRARGPSCRFDSFMFGMRPPV
                     ATRDFAAQTKANLRGLGITTERVGVDCLNLEAVEALRNTGIQLVDGSRAIYQARAVKT
                     TDELQLIRWTTRAKEKGYDLVRAALHEADLPEERLSRIMLDSLLDQGFEAGSEFVSIY
                     ESSKARTHNEPMGTDMVVVDGDLVICDATVAGPGGYYSDFARTFSRGRPTAQSRNRYR
                     EAYQSLQDSLPLIKPGPCTELFARFGQHSSSRIAGLDGFHGVGLCIYESPWLRGGDPP
                     EYAVDLEENMIVALEINHYPVKLEHLLRVTDSGAEILSRYPVDPELVPA"
     gene            2388..3302
                     /locus_tag="VF182_13355"
     CDS             2388..3302
                     /locus_tag="VF182_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX6438119.1"
                     /translation="MSRFVPAPQESRASALAIWSALLAVYLIWGSTYLAIRFTVETAP
                     PFVSAAFRFIVSGACLYTWRRFAGDPKPTLVEWRSAATVGLFLLLGGNGGVVWASQFI
                     PSSLAALLVATVPLWMVAIDSARRGNQRPSVPAIIGILIGFGGVVLLIRFAASDALVF
                     NLFGTTAVLFASLSWAIGSLYARTARLAASPLLATGMEMFAGGVALLAVAILFGEWKH
                     LDLGAISSRSALALAYLTAVGSAAFVAYVWLLRVAPTPLVSTYAYVNPLVAVLLGYFL
                     ANEPMTGRTLFAASLIIGSVVLVNAPKR"
     gene            3425..4030
                     /locus_tag="VF182_13360"
     CDS             3425..4030
                     /locus_tag="VF182_13360"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6438120.1"
                     /translation="MSITLYDASPSANSDRVKIVLHEKELPYQKVTLDLAKKEQKRAE
                     FLKLNPYGKVPVINDNGKVLFESCIINEYLDEQYPNPPLMPKDPYVRGRGRILVDYGL
                     RYMHEPYWALRGEMLKQESERDQTMIDEKRRELRELMRYLEEALGDKPYFLGELSLTD
                     IDLLARFIRFEDYGALPDLSLPRLNSWLQKIKQRPSVKPLL"
     gene            4109..5608
                     /locus_tag="VF182_13365"
     CDS             4109..5608
                     /locus_tag="VF182_13365"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX6438121.1"
                     /translation="MPEASQPFNIIEATIESVHRAYQSGQLTAQRLVQMYLDRIEAFD
                     KKGPAINAIISINPLALGEAERLDAAFKTSGFVGPLHGIPVIMKDQGDAKGMPTTLGS
                     VLFKDYYPQRDAFAVEKLKRAGAVILGKATLGELGGGDTHGSLFGSTRNPYDLERTAG
                     GSSGGSGASVAANYCTVAIGQEGFASIRRPSTWNSVCGMRPTAGLVSRSGVYAGWPSP
                     VGSLGPMSRSMEDLAKLLDVMVGYDPEDPLTARGVGQIPESYTSFLDKNGLKGARIGI
                     LREAMGYATEPESEDFHKIEEVFDRAVDELKSLGAEIVDPIVIPNLKALLAKRSGSLA
                     VEQESFKIYFGRNSGAPYKTREDAMKSPDFAKVVKGSRERWSRSGEPAAHYEYLKARE
                     QLMTNVLKVMADNRLDAIVHKAVEHQPTLIKDGINPPYVDQKGAPHINTFLIFVPAVV
                     VPAGYTRDNLPAGITFLGRPYEEGKMIKLAYAYEQATRHRRPPASTTAL"
     gene            5956..7488
                     /locus_tag="VF182_13370"
     CDS             5956..7488
                     /locus_tag="VF182_13370"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxyl carrier protein"
                     /protein_id="HEX6438122.1"
                     /translation="MNLMKQTGEIRFVDTTLRDGQLSLWASNMTTGMMLPVIDRIDAA
                     GFEAFELISSAFFKKAVRDLKEDLWERTRLVAQRTKKTPLRAIRSRSMLAFQITPGAI
                     AELWLERLAANGISELRTSDPSNTPSYWAEAVAGANRLGLKTILNIIYSISPKHTNDY
                     FLQRIREAAKLDVARICFKDPGGLLTPERTRALVPLILENTNGKPVEFHTHCTTGLGA
                     PSCLEAINAGIRSINTAIPPLAEASSNPSIFNVAMNARALGYSTAIDEEVLKPVTEHF
                     TAIAKEDNLPIGRPVEYDAFHPIHQVPGGMISNFRFQLANLGKLDQLDAVLEEVVRVR
                     AEFGYPIMVTPYSQFVGVQAAINVIVGERYKEITDEMIQYALGRWGEEEARSIDANVK
                     DRILSRPRAKELAKWTLPEISVKEFRAKFGGDGVSDDEKILRYFAGEDYVASMKAAAP
                     ARDYTSTATPLVALISSLAQRKDTTQIFIQRKDFSLRLEKRSAVAASDNVSVDSSEAK
                     SS"
     gene            complement(7502..8770)
                     /locus_tag="VF182_13375"
     CDS             complement(7502..8770)
                     /locus_tag="VF182_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015621624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6438123.1"
                     /translation="MPKHAESVAAQSGVAPVKPVAFAALRHRDFRFFFPATMLAMMAD
                     NIEHVISYWLLYQKFQSPVLAGFAVISHWTPFLLLSVYFGGLADRYDCRKLIQIAQLM
                     YMAVSIAWAFLFFTDSLEVWHACVLLVIHGIAGVFWTPAEQLLIHDIVGPEDLPSAVR
                     LNSTSRQLGILLGPAVGGGLMLWLGAPAGLLINAIIYLPLTIWLMIVPYTGHVRDDAP
                     PRRAMGWKDAIGTVRQIMEHRPIVTMIALGGAASLFVGNAFHAQMPEFAHDLGTEKAD
                     LTYSALLAANAAGAVVGGFVLDGKDWVPPTVRSAILCAILWCVVMAAFAFSYSYPLSV
                     ALLFCAGALNLAFYSTAQTIVQLQAPIDLRGRLIGLFTMSAYGLRAFSGVTVGVVGGL
                     IGIHWSLALSSMILLGITLALFAFALPAQK"
     gene            complement(9080..10174)
                     /locus_tag="VF182_13380"
     CDS             complement(9080..10174)
                     /locus_tag="VF182_13380"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6438124.1"
                     /translation="MMANNPENSAARFRGEIGIHFPLHVLNRYSLPELIALAERSRDT
                     LGPHGFTQVWTNDNLEYRSVLASSAAIVARLPVKLGTAIAVPYFRNPIDFAAAFATMS
                     ELTDGREISLGLGPGSRSILTHHVDRVKPMTIMTELATTLRTLFKGEAIRRSDMPALA
                     DYFHLNAESYTLRFKARAPIRLYYGPSLLKPGVLNLIARYFDGVILQTLYGLTAVEET
                     LNTLADARKQSTLSEPLRKVMLLNASVSRDGQAAKQHAKRFVSHIASGWPNDTLERIG
                     IDPKIMEPVRQAYAQNRGVDYAASITPDEAVERLIIAGTPAQCRERIAELFAFAARHG
                     FFQVCIGVPAGPDIAEVIELWGNEILPSLG"
     gene            10381..10740
                     /locus_tag="VF182_13385"
     CDS             10381..10740
                     /locus_tag="VF182_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438125.1"
                     /translation="MWIILILLWLLPLSLGLVLSARVLAGLPAVSEQEWRALFRDRID
                     FKPSLWSVLVFAVFAVMFFLIGVLEGVVLVNIGSYWLVFVPLLTGLAALLLVLFWLRT
                     EIRGFPMPRRARSIFSQ"
BASE COUNT         2460 a         3069 c         2997 g         2303 t
ORIGIN      
        1 tcttcagcac aggctcggaa aacgaaagaa atctcatccc atgctccaca gcgacatccc
       61 cccaggaatc cataccttca tcgatcacca tatccaaggt gccggagacg attccttccc
      121 ggcgatgtgg attgctcgga cgtactactt catccaccgt accgccccag tcgataaagt
      181 ccttttgcga gatgccgtga cacttaagta aatactttat cgcgaaatcg acgcaatcgt
      241 tgacggctat cgacacattc atcttgggct ttttggcgat gagctcttcc atcgtgcgca
      301 cgcctagctg cggcgaaacc gcgatcacca aacgatccca cgacggaaac accgccagtg
      361 cgcgcagcgg atacttgcgc cggaacggcc ccactcctcg aaccgccatg gacggaatga
      421 tcgaaggatt aagccagacc agatcgagct tgccgctggc aacgagtttg gcgccgttga
      481 gtccgctgca ccacgccggg ctcactccga tcgtgaatgg ataaggcggg ccggaatgcg
      541 ccgagccccc gggcagctcg accatcaatc gaatcgcgct ggtcaacagc gtcggcacat
      601 cttcatagag tccggagaga atcttggtag caatccgcgc gcgcatcaaa ctggcgctca
      661 tcggttttac actgcgatca tcataaacca ctcaacacca ctcctttagt ttttgtttga
      721 accatcagag ctatgctgaa tgattttcgg agtcaacagt ttgcatccgg agtggcgcgc
      781 tatcccacag ctttcggatc gccaagaacg ctgatcattc ttgacgcctg ttcggcgcaa
      841 gcgatatatc aattagctag caggctgcta aatcatcact gcaggctgct caaaaagatc
      901 cagatacaag gcgcgcgaag cacgaaggat gaaaacggaa atctgaaagc ggaattcagt
      961 tttcagcttt cataattcat cctttcccat ggcatatggg gctttttcag cagcctgcta
     1021 cggagagttc atgtattacc ccgacctcag tggtttcggc gtcgataatg accggctcgc
     1081 gtcgcagcgt ttgtcgcgcc tgcaacaagc catgcaacaa cacaatctgg cggcgctgat
     1141 gctcaccgac tggctcaaca ttcgttatgc caccaactcg gtgttcatga tgaatctgcg
     1201 cgccactgcg gtccagcgtt ttgtcctggt gccggcgacc ggcgctccgt taatttgcca
     1261 acgtcaactt tcgagcaggg ctcgaggccc gtcgtgccgc ttcgattcat ttatgttcgg
     1321 catgcgaccg ccggttgcga ccagggactt tgccgcgcaa acgaaagcca atctgcgcgg
     1381 attgggcatc accaccgagc gggtcggcgt cgattgcctg aacctcgaag cagtcgaagc
     1441 attgagaaac accggcatcc aactggtcga tggttcccgc gccatctatc aggcgcgcgc
     1501 agtaaagacc accgatgagc tccaactgat tcgctggaca acccgtgcca aagagaaagg
     1561 ctacgatctg gttcgcgcgg cgctgcatga agcggatctg cccgaagaac gcttgagccg
     1621 gatcatgttg gattctttgc tggatcaggg attcgaagcg ggcagtgaat tcgtttccat
     1681 ctatgaatcg tccaaggcgc gaacgcacaa tgagcccatg ggcacggaca tggtggtggt
     1741 ggatggcgat ctggtgatct gcgacgccac cgtcgccgga ccgggcggct attacagcga
     1801 tttcgcccgt acattcagtc gcggcaggcc gacggcgcag agccggaacc gttatcgaga
     1861 agcttaccaa agcctgcagg attcattgcc gctgatcaag cccggaccct gcaccgagct
     1921 tttcgctcga ttcggccaac atagctcttc gcgaatcgct ggactggacg gttttcatgg
     1981 cgtcggcttg tgcatctatg aatcgccctg gctgcgcggc ggcgacccgc cggaatacgc
     2041 ggtggatcta gaggagaata tgattgtcgc cctcgagatc aatcactatc cggtcaaact
     2101 cgagcacttg ttgcgcgtaa ccgattcggg ggcggagatt ttgtcgcgct atccggtaga
     2161 tccagagcta gttccggcgt gagtggccaa cgcgcaatcc cgacaaccgt caggcgcaat
     2221 tcggcggcag tctcatgcct atcgaggcca cagttcatta tcagcggcct tgagccggct
     2281 tggcgaatcg tgagtttatt cgagccggtc cttcagcctc ggaaccgttc cccgcgagcc
     2341 tgcgaaaatt ttctcaacga gctggtgagc ttcgaaagag cctgtccgtg agccggtttg
     2401 tgccggcgcc gcaagaatcc cgagcttctg cgctggcaat ttggtcggcg ttgttagcgg
     2461 tttacctgat ctggggatcc acctatctgg cgatccgctt tacggtcgaa acagctccac
     2521 cctttgtttc cgcagcattt cgtttcatcg tttcaggagc atgcttatat acctggcggc
     2581 gtttcgccgg tgaccctaaa cccactctag tggaatggcg cagcgccgct acggtcgggc
     2641 tttttctttt gctcggaggc aacggcggcg tcgtctgggc ttcgcagttc attccttcca
     2701 gtctcgccgc gctactggta gctacggtac cgttgtggat ggttgcgatt gactcggccc
     2761 gccggggcaa ccaaagaccg agcgttcctg cgatcatcgg cattctcatc ggcttcggcg
     2821 gcgtcgttct gctgatccgg ttcgcggcca gcgatgcgct tgtcttcaat ctattcggca
     2881 ccacggctgt gctgtttgca tcgctctcat gggccattgg ttcgctgtat gcaaggaccg
     2941 caagacttgc cgcctcaccg ctactggcaa cgggcatgga aatgtttgcc ggcggtgtcg
     3001 cactgctggc ggtcgcaatc ctgttcggag aatggaagca cctggatctt ggcgcgattt
     3061 catcacgctc ggcgctggcg ctggcgtact tgacagcggt cgggtccgcg gctttcgttg
     3121 cctatgtatg gctcttgcga gtggcgccga caccgctggt gtcgacctat gcttacgtca
     3181 atccgctcgt ggcagttttg ctggggtact ttcttgctaa cgagccgatg accggccgca
     3241 cgttgtttgc cgcgagtctg atcatcggct cggtcgtgct ggtcaacgcg cccaaacgat
     3301 agggcgcgac aatccaacca atagaaatgc agcaaccgat tgcaaatcgc cgcgacttct
     3361 aaatataaga ttcctatgat ctgcgatttg tagttttggc gaatttattg ttcgggggat
     3421 caatatgtcc attaccttgt acgacgcatc gccgtcggct aacagcgatc gcgtaaaaat
     3481 cgttcttcat gaaaaggaac tcccctatca aaaggtgacc ctcgatctgg ccaagaaaga
     3541 acagaaacga gccgaattct taaagctcaa tccgtacggc aaggtaccgg tgatcaatga
     3601 caacggcaaa gtgctttttg aatcctgcat catcaatgaa tatctcgacg agcaatatcc
     3661 gaatccgccg ttgatgccga aagatccgta tgtgcgcggg cgcggccgca tcttggtgga
     3721 ttacggtttg agatacatgc atgagcctta ctgggcattg cgcggcgaga tgttgaagca
     3781 ggaaagcgag cgcgatcaga caatgatcga tgagaagcgc cgggaattga gagaactcat
     3841 gcgctatctc gaagaggcac tgggtgacaa gccgtatttt ctcggagagc taagcctgac
     3901 cgacatcgac ctgctggcgc gctttatccg attcgaagat tatggagctt tgccggatct
     3961 gtctttgccg cggctcaata gctggctgca gaagatcaaa cagcgcccat ccgtcaagcc
     4021 gcttttgtag gcgagcgata attgcaacgt gctaaattcc caagcgtgaa gcgttacacg
     4081 acctagtcaa cttaaaggag agaagaagat gccagaagca agccagccct ttaatatcat
     4141 cgaagctact atcgagagcg tgcatagggc ttaccaatcc gggcagctta cggcgcagcg
     4201 cctggtgcag atgtatttgg atcgcatcga ggctttcgat aagaaaggcc cggccatcaa
     4261 tgccatcatc tcaatcaatc cactggcgtt aggcgaagct gaacgccttg acgccgcttt
     4321 caaaacatcc ggctttgtcg gtccgctaca cgggatcccg gtgatcatga aggatcaagg
     4381 cgatgccaag ggaatgccga cgactctggg ctccgtgttg ttcaaggatt actatccgca
     4441 gcgcgacgcc ttcgccgtcg aaaagctcaa acgggcaggc gccgtgatcc ttgggaaagc
     4501 cacgctcggc gagttaggcg gcggcgacac ccacggttct cttttcggtt cgacgcgcaa
     4561 tccctatgac ctggaacgca ccgcgggcgg atcctcgggc ggctccggag caagtgttgc
     4621 cgccaattat tgcacggttg ccatcggcca ggagggcttt gcatcgattc gccggccatc
     4681 gacgtggaat tcggtgtgcg gcatgcgccc aaccgccggc ttggtcagtc gcagcggagt
     4741 ctacgccggc tggccatccc cagtcggatc cctgggaccg atgtcccgtt cgatggaaga
     4801 tctggccaag ctgctcgatg tcatggttgg ctatgacccg gaagatcctc tcaccgccag
     4861 gggtgtcgga cagattccgg aaagctatac gagctttctc gataagaacg gcctcaaagg
     4921 cgcgcgcatc gggattttgc gcgaagccat gggttatgcg accgaacctg agtcggaaga
     4981 cttccacaag atcgaggaag ttttcgacag ggccgttgac gagttgaaaa gcctgggagc
     5041 ggagattgtc gatccaatcg tcattcccaa tttgaaagcc ttgcttgcca agcgctccgg
     5101 cagcctagcc gtcgaacaag aatcgttcaa aatttacttc ggcagaaatt ccggcgcgcc
     5161 gtacaaaacc cgcgaggacg cgatgaagtc gccggacttc gccaaagtcg tcaaaggctc
     5221 gcgcgaaaga tggagcaggt ccggcgaacc ggctgcgcat tacgaatacc tcaaggcgcg
     5281 cgagcaactg atgaccaacg tgttgaaggt catggctgac aaccgtctgg acgcgatcgt
     5341 tcacaaggcg gtggagcatc aaccgacctt gatcaaagac gggatcaacc cgccgtacgt
     5401 ggatcagaaa ggcgcaccgc acatcaatac gtttctgatc ttcgttcctg ccgtcgtggt
     5461 tcccgccggc tacacacgcg acaatttgcc cgcgggtata accttcctgg ggcggccgta
     5521 tgaggagggc aagatgatca aactggccta tgcctatgag caggcaacgc gccaccgccg
     5581 gccgccggcc agcaccaccg ctttgtgaat tatgatgctc gtgttcattt cgatttcaag
     5641 aatgcccgag gagcgagcct ctccggtcgc gggcaatggg cctgtgccat gaaggaggcc
     5701 tacaacctgt gtggtagatg attgtacagt gggaggaacg aagcttattg ccaactcgat
     5761 aaaagtcgcg gacttttcgt tcctgtgccc gctcgcttcg aggctcactc ctcgggcaac
     5821 acagtgtgat gattttcttc gaaagatgaa taatccgggc ctgcagccaa agagctattg
     5881 tctttgctcc ggaaggcttg ccgcttgatc gtattacaat tggaattgcg ggatcggggt
     5941 agcaaataat tgactatgaa cctcatgaaa caaaccggtg aaattcgctt cgtcgatacc
     6001 accttgcgcg atggtcagtt gagtctgtgg gcgtccaata tgactacggg catgatgctg
     6061 ccggttatcg atcgtatcga cgcagccggc tttgaagcat tcgagctgat ctcctcggca
     6121 tttttcaaga aggcggttcg cgatctcaaa gaagatctct gggaacgaac ccgattggtg
     6181 gcgcaacgga cgaagaaaac gccgctccgc gccatccgca gccgctccat gctggcgttt
     6241 cagatcaccc ccggcgccat cgccgagctc tggctcgagc gattagccgc taacgggatc
     6301 tccgagttgc gcacctccga tccttccaat actccatcgt actgggcgga agcggtggcc
     6361 ggcgccaatc ggctcggcct caaaacgatt ctcaatatta tttattcgat atcgccgaaa
     6421 cataccaatg actatttcct tcagagaatt cgcgaagccg ccaaactcga cgtggcgcgc
     6481 atctgcttca aagacccggg aggactcctc acgccggaac gaacccgcgc gttggttcca
     6541 ctgattctcg aaaataccaa cggtaaaccg gtcgaatttc acacccactg cactaccggg
     6601 ctcggcgcgc catcctgcct ggaggcgatc aacgcgggaa tccgctcgat caacacagcg
     6661 atcccgcctt tggcagaagc ttcctccaac ccgtcgatct tcaatgtcgc gatgaacgcc
     6721 cgggcgctgg gttactcgac agcaatcgat gaagaagttc tcaagccggt gaccgagcat
     6781 ttcaccgcga tcgccaaaga agacaatctt ccgatcggca ggccggtcga gtatgacgcc
     6841 tttcacccga ttcatcaggt ccccggcgga atgatttcca acttccgctt ccaactggca
     6901 aatctcggca agctcgatca attagacgcg gtgctcgagg aggtagtgcg ggtccgcgcg
     6961 gagtttggct atccgatcat ggtgacaccc tactcgcaat ttgtcggcgt gcaggcggcg
     7021 atcaacgtca tcgtcggaga acgctacaaa gaaattaccg atgagatgat tcagtacgcg
     7081 ctcggccgct ggggcgaaga ggaagcgcgc tccatcgatg ccaatgtgaa agataggatt
     7141 ctcagccggc cgcgcgccaa ggagctcgcc aaatggacgc tgccggagat ttcagtcaaa
     7201 gagtttagag caaagtttgg cggcgatggc gtctcggacg atgaaaaaat tctccgctac
     7261 tttgccggag aagattatgt tgcaagtatg aaggcggcgg cgccagcaag ggattacacg
     7321 agcacggcga cgccactagt agcgctgatc agctcattgg cgcaacgcaa agataccacg
     7381 caaatcttca ttcagaggaa ggatttctca ttgcggcttg aaaaacgatc ggccgtcgct
     7441 gcttcagaca acgtttcagt cgacagttct gaagcgaaat cgtcctaacg gcgcggggca
     7501 atcacttttg cgccggcagc gcaaaggcga ataatgccag agtaattccc agaagaatca
     7561 tcgagctcag cgccagcgac cagtggatgc cgatcaagcc gccgaccacg ccgacggtca
     7621 cgccgctgaa ggcgcgcagg ccataagccg acatggtaaa tagaccgatc agccggccgc
     7681 gcaaatcaat cggcgcctgc aactgaacga tggtctgcgc cgtcgaatag aacgcgagat
     7741 tcaacgcccc ggcgcaaaac agcagcgcaa cggataaggg atagctgtag gaaaaagcga
     7801 aagccgccat caccacgcac cacaagattg cgcagaggat tgccgacctc accgtcggcg
     7861 gcacccagtc cttgccatcg agcacgaacc cgcccactac cgcccccgcg gcattcgcgg
     7921 caagcaaagc gctgtacgtg agatctgctt tttccgtgcc gagatcatgg gcgaactccg
     7981 gcatctgggc gtgaaacgca ttgccgacga acagcgacgc agcgccaccc agcgcgatca
     8041 tcgtcacaat cggtcggtgc tccatgatct gccgcaccgt gccgatggca tctttccaac
     8101 ccatcgcgcg ccgaggcggc gcatcgtcgc gcacatggcc ggtgtaagga acgatcatca
     8161 gccagatagt caatggcaga tagataatcg cattgatgag caacccggcg ggcgcgccga
     8221 gccacagcat caagccgccg cccaccgccg gaccgagcaa aattcctaat tgccggctcg
     8281 tcgagttgag ccgaacagcg ctcggtaaat cttcgggtcc gacaatatcg tgaatcagca
     8341 gttgctcggc aggcgtccag aaaacgccgg cgatgccgtg aatcacgagc agcacgcagg
     8401 cgtgccaaac ctcgagacta tccgtgaaaa agagaaatgc ccaagcgatc gaaacggcca
     8461 tatacatcag ctgcgcgatc tgaatcagct tgcgacaatc gtaacggtcg gccaagccgc
     8521 cgaagtaaac agagagcaag agaaacggcg tccagtgact gatcaccgcg aaacccgcca
     8581 gcaccggcga ctggaacttt tgatatagga gccaataact gatcacgtgc tcgatgttgt
     8641 ccgccatcat ggcaagcatg gtggcgggga agaaaaaacg aaaatctcgg tggcgcagcg
     8701 cagcgaaagc cacgggttta accggtgcga ctccactctg cgcggctacg ctttcggcgt
     8761 gcttgggcaa ttctcctcca gagatgagcg cgggactaaa agctgatcgt agaactttcg
     8821 agagtagcca atcttccaaa ccgatgcaaa tcagtgggag gcggatcggc aacttattat
     8881 ggatgaacgg ctaacgtttg gaatgaggat gtgaaaagct tgcaccgcag ttatctaaaa
     8941 ccatcgcggc caattgtcca agacaactcg cgacagacaa aaacgctcgt gaccaaaatc
     9001 aggatcggtt tcaccggatc gagtgcgccg agcccgcatt caccaacttc acggctcccg
     9061 cagtaatcga cttcgctttt tagccgagcg aaggcaggat ctcgttaccc catagctcga
     9121 tgacttccgc gatatccggt cccgcgggca cgcctatgca cacctgaaag aaaccatgcc
     9181 gcgccgcaaa ggcaaagagc tcggcgatgc gttcgcggca ttgcgccggt gttccggcga
     9241 taatcaatcg ctcgaccgcc tcatccggag tgattgacgc ggcataatcg actccacgat
     9301 tttgcgcgta ggcctggcgc accggttcca tgattttggg atcgatgccg atgcgttcca
     9361 acgtatcatt gggccaaccg gaagcgatgt gcgaaacgaa acgcttggca tgttgtttcg
     9421 ccgcctgccc atcccgggac acagaagcat tgagcagcat gacttttcgc aacggctcgc
     9481 tcagagtcga ctgcttgcgc gcatcggcaa gtgtattcaa cgtctcttct accgccgtga
     9541 gaccgtaaag cgtttgcaga atcaccccat cgaagtagcg cgcgatcaga ttcaacacgc
     9601 ctggcttgag caggctaggg ccataataga ggcgaatcgg cgcccgggct ttgaagcgca
     9661 acgtgtagct ttcagcattc agatgaaaat aatcggccag cgccggcata tcgctgcgcc
     9721 ggatcgcttc acccttgaag agcgttcgta gcgtggttgc gagttctgtc atgatcgtca
     9781 tcggtttaac ccggtcgacg tgatgggtca aaatagatcg tgacccaggt cctaaaccga
     9841 gcgagatttc ccggccgtcg gttagctcgg acatggtcgc aaacgccgcg gcaaaatcga
     9901 tcggattacg gaagtacggc accgcaatcg ccgttcccag ttttaccggc agccgcgcta
     9961 ctattgctgc cgagctcgcc agcacactgc gatattcgag gttgtcgttg gtccatacct
    10021 gagtaaagcc gtgtggcccg agagtatcac gcgagcgttc ggccagtgcg atgagttcgg
    10081 gtagcgagta acggttgaga acatggagcg gaaaatggat gccgatttcg cccctgaatc
    10141 gtgccgcgga attttcggga ttattcgcca tcatacgcga gggctatcac accggatcga
    10201 aaagagtcaa tgaagatgag cgatgctaca ggaccgaaca tgctcaaatt ctacatctca
    10261 ttgaactgtc gcaaatccgt gacacgaaac cgccgctccg caaacttcgt caatgaactc
    10321 caaagcttac ccgtggaata tgctttgcaa ccaaccattg cttaggaagg taggtgtctc
    10381 atgtggatca ttcttatcct actctggttg cttcctctct cattgggact cgttctgtcg
    10441 gcgcgagtac tggctggatt acctgcagtt agcgaacaag aatggcgcgc actttttcgc
    10501 gaccggatcg acttcaaacc ttcactgtgg agtgtcctgg tgtttgcagt ttttgcagta
    10561 atgttttttc tgatcggcgt gctcgaaggt gtcgtgttgg tgaatatcgg gtcgtactgg
    10621 ttggtgttcg tgccgctttt gaccgggctt gcagcactgt tgctcgtgct attctggctt
    10681 cgcacggaga tccgcggctt tccgatgccg cggcgcgcac gtagtatttt ttcgcagtaa
    10741 gtttgttgcc gcgtgggtga aaaggatttg ccattagttc ttacgctcac aaaaaagagg
    10801 cggggataac cccgcctctt tgatcacgc
//
LOCUS       DASUVS010000244         9339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1412267, whole genome shotgun sequence.
ACCESSION   DASUVS010000244 DASUVS010000000
VERSION     DASUVS010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9339
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412267"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..980)
                     /locus_tag="VF182_13390"
     CDS             complement(120..980)
                     /locus_tag="VF182_13390"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6438126.1"
                     /translation="MAERPFTWPNKARVAFVLGIAFEAWDRTKPTTGRGLQRGSIPAN
                     AVCRRDYSTETFREFGAKTGLRRLLDICDRYGLKVSMPINGLTCEYYPELVKEAHQRG
                     HEMIAHGWDQGQYLYMLTREQERDNIVKTVEAITRVTGERPTGWSSPGVRSTDNTLEL
                     NVEAGLLYNCDYHDDEMPYPRRIGDKLLMMIPHQYTINDHRMEEEGTRHEFFDKFKDE
                     FEYRYKIGADNPTMMNVISHAYLSARIPVIDTFEDIIAYAKKHKDVWFARRGDIAAWA
                     RNYFEENEAL"
     gene            1309..3327
                     /locus_tag="VF182_13395"
     CDS             1309..3327
                     /locus_tag="VF182_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX6438127.1"
                     /translation="MDSRRRLKSTVKKAAKKTAFRVEEATIGGVHRAFRAKKLTAMEL
                     VNLYLARIKAYNGVCVRGGVDSATGLQLGDITPIPNAGQLNAFITLNLKKDKRIKLGF
                     SEKLKRTHTGGDDEKYPDALDRARELDRYFARTGKFAGPLHGIPVAIKDNYDTFDMRT
                     TAAAAAEYADDRPPDDATMVAKLRAAGAILLGKTNMDEYAPAGIARSAFGGQTCNPYD
                     TTRSPGGSSGGSAAAVAANLAMCALGTDTSGSVRFPSSCCSLVGLVASQGLVSRDGIV
                     PLSFTRDRGGPICRTVQDTAILLEVLAGYDPKDPVTAACAARPKISYARHAGGKSLRG
                     KRLGVLRDLMIEASMADRENIRVANEAIAAMKKAGATIVDPVNVDKTIADLVSYLEPG
                     WLRRNFSAAFPESDKPIDRIVSMAFDHALIPSGARGVNLRMLAAQPRGHEAHYAINRY
                     LRERGDAKFKDLQDMLVMPMFSGSLENLKRAFSDDAETLDTATQTDHLIRMQTLRRII
                     LQVMAHYSLDVLVYAYTTIPPHIILTNRLAKTLETHTEPRILKAETVMSDPTLVPGEP
                     VLKTDLDTYRGSGSSFAVSLSPETGFPAIVVPAGFTGEVYDRVPDHNDPNGSRLEGPK
                     QVELPVALEFLARPFDEATLFEIASAFESITLHRRPPPGFRELADKTG"
     gene            3694..4239
                     /locus_tag="VF182_13400"
     CDS             3694..4239
                     /locus_tag="VF182_13400"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEX6438128.1"
                     /translation="MAEPAIGEVGEKNPNVVIVWRAFELRPDPVPTLDPNGEYLHRVW
                     GSSVYPLAEQLRITIKLPPVQPRSRRAHQAAHWARRHGKFREYNDALFRAFFQRGEDI
                     GNNDVLTRLASDMALDGNDLRAALDNDLHLENVLADEREAKKLGLRGVPAFIANRKIA
                     LSGVQSVESLEKFVEHARSLS"
     gene            4252..4407
                     /locus_tag="VF182_13405"
     CDS             4252..4407
                     /locus_tag="VF182_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438129.1"
                     /translation="MRALAGASKILHTDCEYFYKFLGKSPATDGSIPDAAYNGEIEAL
                     EPRWLRA"
     gene            complement(4454..4864)
                     /locus_tag="VF182_13410"
     CDS             complement(4454..4864)
                     /locus_tag="VF182_13410"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6438130.1"
                     /translation="MSPLQGIKGMRHIALKVRNIAKAKSFYQSMLGMDVVWEPDPENV
                     YLSSGYDNIALHQVTAGFPSGAAEQQLDHFGFIVESIERVKELEQEFRSAGVTIVHPF
                     KIHRDGSASFYCADPDAIVIQMIYEPQLSPQALK"
     gene            5148..7547
                     /gene="ptsP"
                     /locus_tag="VF182_13415"
     CDS             5148..7547
                     /gene="ptsP"
                     /locus_tag="VF182_13415"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX6438131.1"
                     /translation="MAKTKKTGREVHPHLTILEDISTLIAHSHDLQDTLQNIVTTVRD
                     RMEAEVCSIYILDPQKKRLTLRATMGLDPESVGKVSMAIGEGLSGLVIERMEPVMVVD
                     ALAHPRYRYFPETGEERFHSFLGVPLFDNKSPLGVLVVQTSRRRQFSRDEIRLLTTIS
                     AQAASIIVQARLLESLRNKERERKEYQKRMVDALRKLRSYEGRRREKTSKTSHKWRGR
                     LVGLAASPGFGRGRAFVLNPRMELTAIEKKRARNPQREIERFRGAVERGIEQINVVKQ
                     RMTNLISREEGAIFDVYRLILEDPAIIQQIEAQIRKENYVAEYAVRVVFEQYLSSIAQ
                     IQDEYLRERTADVKDAAQRLLENLSGTQDQHIDVPSDAVLVAEDLSPADLSVLEGDRF
                     RGIVLSTGGVTTHASILAKSFEIPSVVAVEGLMDIVHQGDAVIVDGNSGVLYVNPNPE
                     VAREYDRLERDYAALNRELQELKDEPAETIDGHRVSLYANIGLMSDIAFAHLHGAQGV
                     GLYRTEIPFLAHRDFPSEEEQYSLYKRVVEGMAGKPVTVRTLDIGADKYPSYMRSTKA
                     EPNPFLGWRSIRISLDVEEIFKTQLRAILRAGTLGRVRLLIPMISSLEEVLKVKELLA
                     EAKDELQRESTPFDLNMELGIMVEVPAAVQIAERLLREVDFLSIGTNDLIQYILAVDR
                     SNRKVANLYEPLHPAVLAALYSTIQAGKREGKRVGMCGEMASDPIYALVLLGMGLEEF
                     SMGSLFVPVVKKTIRSVTYEAAKSMARIVLEMDTATEIKRYLFEQMRQLGMVELMEMY
                     H"
     gene            7666..8772
                     /locus_tag="VF182_13420"
     CDS             7666..8772
                     /locus_tag="VF182_13420"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEX6438132.1"
                     /translation="MHKIANFDDWIDYFRVWQKDIGYDPALLGDYKFETKLGELHSSD
                     IEFGDFKGQPKWQRIGQIPNQSIRDALMNLIVYQGDTEFASVEQQKNLLDTAPTEYDR
                     KALTRVNSEEMRHGWQMCYLLVNYFGDSGKLEARKLLERRAFRGDRLLGSFNAPVKNW
                     LDFFTYTEFVDRDGKYQLTMLSHSAFAPLAESVTAMLKEEHFHMFTGHTGLTRVLRAG
                     QIPVAIVQKYFNKWLSTAYDLFGTDNSSSANWAYVWGLKGRYDEHEATAAADKDRLND
                     LARDHYMAESSKLIDQLNQLIPEGQPKLSAPDLKFNRSIGEHAGKTFSVTGELLSAEA
                     YKKHVAEALPTAEDEQTLNEIMRGKEWVSQIQMN"
     gene            8845..9159
                     /locus_tag="VF182_13425"
     CDS             8845..9159
                     /locus_tag="VF182_13425"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX6438133.1"
                     /translation="MCLVCQNIDCKARGSEQLMGELEKRVAAKGLEDVEVKPYMCFGA
                     CQEGPNIVLYPEKSWYANVKVEDLDEIAEHIAGGPHVKRLDTIDPSLKELIYQLLDTG
                     IV"
BASE COUNT         2172 a         2501 c         2628 g         2038 t
ORIGIN      
        1 cattcgctct ggagggcttg ccgcttgatc gctgtgtcaa tttgaactgc gcaatccggg
       61 ttcacggacg cgagtactag gatccgcctg gcggctgagt cattttacca tcggaactgc
      121 tagagagcct cgttctcctc aaaatagttt ctcgcccatg cggcgatatc gccgcgccgg
      181 gcaaaccaga cgtctttatg tttcttggca tacgcgatga tgtcttcaaa ggtatcgatg
      241 acgggaatgc gcgcgctcaa ataagcgtga gagatcacgt tcatcatcgt cggattgtcg
      301 gcgccgatct tgtagcggta ttcgaattca tctttgaatt tatcaaagaa ttcgtgcctc
      361 gtgccttcct cctccatgcg gtggtcgttg atcgtgtatt ggtgcggaat catcatcagc
      421 aatttatcgc caatcctcct ggggtaaggc atttcatcgt cgtgataatc gcaattgtat
      481 aaaagacccg cctcgacatt gagctcgagc gtgttatcgg ttgaacgaac tccgggcgat
      541 gaccaaccgg tcggtcgttc ccccgtcacc ctggtaatcg cttctacagt tttgacaatg
      601 ttgtcgcgct cctgctcgcg ggtgagcatg tacaggtact ggccctgatc ccagccgtgg
      661 gcaatcatct cgtgcccgcg ctggtgcgcc tccttgacca gttctggata atactcgcag
      721 gtcaatccat tgatcggcat actgactttg agaccgtatc gatcacagat atccagcagg
      781 cgccgcaaac ccgttttggc gccgaactcg cgaaatgttt cggtggagta atcgcgcctg
      841 cagacggcgt tcgcgggtat cgagccgcgc tgtaatcccc ggcccgttgt cggcttggtt
      901 cggtcccaag cttcaaatgc aatgccgagc acaaaagcga cccgggcttt attcggccat
      961 gtgaaggggc gctccgccat gtaagtttct ccgactgata caggtcgtca gacaaccaga
     1021 gcttatagcg aaagttttcg aacacagcaa ggtgtaaatc cgaacttgag agtatctgac
     1081 aatttctttt agattgatcc gcatggtgtg gttgaacatg gtatgaacgg agttgcccag
     1141 cttgcgaaaa atcctcaatt gtgatggtgg ctggtgttga ttcagtgagc agccgacaga
     1201 gttcgagatg gtgatcaatt tgaaaattcg gaagcagctc gggttggcaa tcccgcaaac
     1261 cgtaccggca cggccggaca gagtcatcaa ataggaacag cagtgatcat ggattccagg
     1321 cgaagattaa aaagcactgt aaaaaaggcg gcaaagaaaa ccgccttccg tgttgaggaa
     1381 gcaaccattg gcggagtgca tcgagccttt cgcgcgaaga agctcacggc aatggagctg
     1441 gtgaatcttt atctcgcccg catcaaggcc tacaacgggg tctgcgtgcg cggtggtgtc
     1501 gacagcgcca ccggcctcca gctgggagac atcacgccga ttccaaatgc cgggcagctc
     1561 aacgccttca tcactcttaa cctgaaaaaa gacaagcgca tcaagcttgg tttttcagaa
     1621 aaattgaaac gcactcacac cggaggcgac gacgaaaaat atcccgacgc gctggatcga
     1681 gcgcgcgagc tcgaccggta cttcgctcgc accggcaagt ttgccggccc gctccacggc
     1741 atcccggtcg cgatcaagga caactacgat actttcgaca tgcgcacgac ggcggccgcg
     1801 gcggcggagt atgccgatga ccgcccgccc gatgacgcca ccatggtcgc aaagttgcgc
     1861 gccgctggtg caatcctgct cggcaagacc aacatggatg agtacgctcc tgcgggaatt
     1921 gcgcgtagcg ccttcggcgg ccagacctgc aatccctacg atacgacgag aagtcccggg
     1981 ggatcgagcg gcggctcggc cgcggcagtg gcagccaacc tcgccatgtg cgcattgggg
     2041 acggacacat ccggctcggt gcgttttcca tcatcgtgct gcagcctggt gggtctggtt
     2101 gcgagccaag ggctggtgag ccgcgacggc attgtgccgc tgtcatttac cagggatcgg
     2161 ggcgggccga tctgccgcac cgtgcaagac actgcgatcc ttctggaagt tctcgccggc
     2221 tacgatccca aagatcctgt caccgccgct tgcgcagcgc ggccgaagat ttcctatgcg
     2281 cggcatgccg gtggaaaatc gctacgcgga aaaaggctcg gtgtgctgcg cgatctgatg
     2341 atcgaagcga gtatggccga tcgggaaaat attcgcgtcg ccaacgaagc gatcgccgcc
     2401 atgaaaaagg ccggcgccac gatcgtcgat ccggtgaatg tcgataaaac aatcgccgat
     2461 ctggtttcct atctcgagcc cggctggctg aggagaaatt tttccgccgc gtttcccgag
     2521 tccgacaagc ccatcgatcg aatcgtgtcg atggcgttcg atcacgccct gattccttcc
     2581 ggcgcccgag gcgtgaacct gcgtatgctg gccgcgcaac cgcgcggcca tgaagcgcac
     2641 tacgcgatca accgctacct gcgtgagcgc ggcgacgcca agttcaaaga tctgcaagac
     2701 atgctcgtca tgccaatgtt ttcgggcagc ctggaaaatc ttaaaagagc gttcagcgac
     2761 gatgccgaaa ctctggacac ggcaacgcag actgatcact tgatacgcat gcagacctta
     2821 cgtcggatta ttctccaagt catggcgcac tatagtctcg acgttctggt ttacgcctac
     2881 acgacgattc cgccgcacat catcctgacg aatcgcctgg cgaaaacact ggagacgcac
     2941 actgagccgc gaattctgaa agccgaaaca gtcatgtccg atccgaccct tgtacccggc
     3001 gagccggtgt tgaaaaccga cctcgatacc taccgcggct ccggctcgag cttcgcggtg
     3061 agtctcagcc ctgagaccgg ctttccggcc atcgtcgtgc cggccggatt caccggcgaa
     3121 gtctacgatc gcgtgccgga ccataacgat ccgaacggca gccgtctgga agggccgaaa
     3181 caagttgagc tgccggttgc cctggagttc ctcgcgcggc ctttcgatga agcaacgctc
     3241 ttcgagattg cgtccgcgtt tgaaagcatc acacttcatc gccgcccacc gccgggattc
     3301 cgcgagcttg ctgataaaac gggttagaga gcggctcgaa cgcctacgtt ccggcttttc
     3361 gagaagtgat tttccaggac gataccgccc cattcgatat cggcctgtcg cgggtggata
     3421 aagcctgatt ctatggaccg gcttgaacgg agcgcagcgg ttgaaggact tgaacgattg
     3481 gaacaaatcc aaggttgtac agtcgggcgc ggccggaacc tggcagagcg aagcagcaat
     3541 agctactcaa aatttaccag ccgaactgcg actccgcctg ccgggcgatt tccctgcgat
     3601 cctctggaac gcgcggattg cggagttaga gtgaagattg tggaaccagt aaagctcgaa
     3661 gtgttttcgg attacgtttg acccttctgc tggctggcgg agccggccat cggtgaagtg
     3721 ggcgagaaaa atccgaacgt tgttatcgtc tggcgcgcat tcgagctacg ccccgaccct
     3781 gtgccgacgc ttgatccgaa cggtgagtat ttgcacagag tctggggtag ttcggtctat
     3841 ccgctcgccg aacagttgag aatcaccatc aagcttccac ccgtacagcc gcgctcccgc
     3901 cgcgcgcacc aagccgcgca ctgggcgcgt cggcatggca agttccgcga gtataacgac
     3961 gcgctttttc gcgcgttctt ccaacgcggc gaggacatcg gcaataacga tgtgcttact
     4021 cgtttagcgt cagatatggc gctcgatggc aacgacttgc gagcggcgct cgataacgat
     4081 ttgcatttgg aaaacgtgct cgccgacgag cgggaggcaa agaagctcgg actccgcggc
     4141 gtgcccgcct tcatcgcgaa tcggaaaatc gccttaagcg gcgtgcaatc tgtggaaagt
     4201 ctcgagaagt tcgtcgaaca cgcgcgctcg ttatcgtaaa acgcgaacta tatgcgtgca
     4261 ctggccgggg cgtcgaaaat cctgcacacc gattgcgaat atttctacaa attcttagga
     4321 aaatctccgg ccacggatgg aagtattccg gacgccgcat acaacggtga gattgaagct
     4381 cttgagccgc gttggctgcg cgcttagggg tgcttgtcta cgaggaacac tggagtttaa
     4441 attcgatccc cgatcacttg agcgcttggg ggcttagctg aggttcatag atcatttgga
     4501 tgacgattgc atcgggatcg gcgcagtaga aagaggcgct gccatcgcga tggatcttaa
     4561 atgggtggac tatggtgacg ccggccgaac ggaactcctg ttcgagctct ttaacccgct
     4621 cgatactttc tacaatgaag ccaaaatggt ccagctgctg ctctgctgct ccagacggaa
     4681 agccggccgt tacctggtgc agagcgatat tgtcgtaacc cgatgagaga tagacatttt
     4741 cggggtccgg ttcccaaacc acatccatgc cgagcatgct ctgataaaaa cttttcgcct
     4801 tggctatatt tctgaccttg agcgctatgt gacgcatgcc tttgatgccc tggagaggac
     4861 tcatggcagt aatataccat actgccgtgg caacgctgca atcatgtcag cggtaaccgg
     4921 caggctatta tgttaatgcc cgaggagact tcttcggcag ccaaagattc atcgaagcct
     4981 gcctgcttcg ccgtctgtga cagacctgag tacctggacc aaatgccgtc agctttccca
     5041 tcatttttcg gccatcgcac gtaggcatac tggcctccgt ctgttagttc actggcgaaa
     5101 aagtgaaatt tatgctctat aaaggagtct ctccaccgtc tgcaaacatg gcaaaaacaa
     5161 aaaaaaccgg ccgcgaagta caccctcacc tgacgattct ggaagatatc agcacgctga
     5221 ttgctcattc tcacgatctc caagacacct tgcagaacat cgtgacaacg gtccgcgacc
     5281 ggatggaggc tgaggtttgt tcgatttaca tcctcgaccc gcaaaagaag cgtctgactt
     5341 tgcgcgcgac gatgggatta gatccggaat ccgtcggtaa agtgtctatg gccatcggcg
     5401 aaggtttaag cggtctggtg atcgagcgaa tggagccggt gatggtggtg gatgctctgg
     5461 cgcacccgcg gtaccggtat ttccccgaaa ccggcgaaga gcgttttcac tctttcttag
     5521 gcgtgccgct gttcgacaat aaatcgccgc ttggcgtgct cgtcgtccaa acctcgcggc
     5581 gacgccagtt ctcgcgcgat gagatccgtt tgttaaccac gatttcggct caggccgcca
     5641 gtattatcgt ccaagcccgg ttattggaat cactgcgcaa caaagaacgc gagcgaaaag
     5701 aatatcagaa gcgcatggtt gatgcgctgc gcaagcttcg ctcctacgag ggccggcgcc
     5761 gcgagaagac ctcgaaaact tcgcacaaat ggcgcggccg tttggtcggt cttgcggcgt
     5821 cgcctggatt tggccgcggc agagccttcg tcttgaaccc gcggatggaa ctcaccgcaa
     5881 tagagaaaaa acgcgcccgc aatccgcaac gtgaaatcga aagatttcgc ggcgccgtgg
     5941 agcgcggaat cgagcaaata aacgttgtca aacagcgaat gacgaatctg atctcacgcg
     6001 aggagggcgc catcttcgat gtttatcggt tgatactgga ggatccggcc atcattcaac
     6061 agattgaagc gcaaatccgc aaagaaaact acgtcgcaga atatgccgtg cgggttgtct
     6121 ttgagcagta tttaagctcg atagcgcaaa ttcaagacga atatttgaga gaacgaaccg
     6181 cggatgttaa agatgcggcg cagaggctgt tggaaaatct ttccgggacc caggatcagc
     6241 acatcgacgt gcccagcgat gccgtgctgg tggccgaaga tctttccccg gcggatctga
     6301 gtgtgcttga aggcgatcgc ttccgcggca tcgtcctgtc aaccggcgga gtaacaacgc
     6361 acgcttctat tttggctaaa tcgtttgaga ttcccagcgt agtggcagtc gagggtttga
     6421 tggacattgt gcatcaggga gacgccgtca tcgtcgatgg caattccggc gtgctttacg
     6481 tcaatcctaa tcccgaagtc gcccgcgaat acgatcggct cgagcgcgac tatgcagcgc
     6541 ttaaccgcga gctccaggaa cttaaggacg agcctgccga aaccatcgat ggtcatagag
     6601 tttcgcttta cgcaaacatc ggcctaatga gcgatattgc tttcgctcat ctccatggcg
     6661 ctcagggagt gggcctctat cgaactgaga ttccgttttt ggcccatcgg gattttccca
     6721 gcgaggagga acaatactcg ctctacaagc gtgtggtcga aggcatggct ggtaaaccag
     6781 tcacggttcg gaccttagac attggcgccg ataagtatcc atcctatatg cgcagcacca
     6841 aagccgagcc taatccgttc ctcggttggc gctccattcg aatatcgctg gatgtcgaag
     6901 aaatttttaa gactcagctt cgggcaatcc tgcgggcagg aacattgggg cgcgttcgcc
     6961 tcctgatacc gatgatatcc agcctcgaag aagtcctcaa agttaaggaa ttgctagccg
     7021 aggccaaaga tgagctgcag cgagagtcca ccccgttcga cctcaacatg gagttgggca
     7081 tcatggtgga ggttccggcg gcagttcaaa tcgctgagcg actcttgcgc gaggtcgatt
     7141 ttctgagcat cggcaccaac gatttgatcc aatacattct tgcggttgat cgcagcaacc
     7201 gcaaagtggc gaatctctat gagccgcttc atcccgctgt tctcgccgcg ctctattcca
     7261 cgatccaggc gggaaagcga gaaggcaaaa gagtcgggat gtgcggcgag atggccagtg
     7321 atccgattta tgcgctggtg ctgctcggca tgggcttgga agaatttagt atgggttctc
     7381 tattcgtgcc tgtggttaaa aaaaccattc gttcggtgac ctacgaagcg gccaaatcga
     7441 tggcgcggat tgttctcgaa atggataccg caaccgaaat caagagatac ttgttcgagc
     7501 agatgcgaca acttggtatg gtcgagttga tggagatgta tcattagagt aggacgctgc
     7561 ggagcggcct aaaattcctt ttaacggggt tgattctaag gatgccgtag gttagattta
     7621 attcgaacga gcgttcgaat tagacccttc cgaaaggatt gaacaatgca taaaatcgca
     7681 aatttcgacg attggatcga ttacttccgt gtctggcaaa aagatatcgg ctatgacccg
     7741 gccctgctgg gcgattataa atttgaaacc aagctgggcg agctccacag ctcggacatt
     7801 gaatttggcg acttcaaggg gcagccgaaa tggcaacgga tcggacaaat accgaatcag
     7861 tcgattcgcg atgcgctgat gaatttgatc gtctatcagg gcgataccga atttgcttcc
     7921 gtggaacagc aaaaaaacct gctcgacacc gcccctacgg aatacgatcg taaagcgctc
     7981 acccgtgtaa acagcgaaga gatgcgccac ggctggcaga tgtgttacct gctggtcaac
     8041 tacttcggtg attcgggaaa attggaggcg cgtaagctgc tcgagcgaag agcgtttcgc
     8101 ggcgaccggc tgctcggatc tttcaatgct ccggtgaaga actggctcga tttctttacc
     8161 tacacggaat ttgtcgaccg agacggaaag tatcagttga cgatgctgag ccattcggcg
     8221 ttcgcgccct tggccgaaag cgtgaccgcc atgctcaaag aagagcactt tcacatgttc
     8281 acaggccaca ccgggctcac ccgcgtgctt cgcgccggac aaattcccgt tgcgattgtc
     8341 caaaaatatt tcaacaaatg gttgtccacc gcctatgatc tgtttggtac cgataattca
     8401 tcatcggcga actgggccta tgtctggggc ctgaagggaa gatacgatga gcatgaagcg
     8461 accgcggcgg ctgacaagga tcggttgaac gatttggccc gcgaccacta tatggccgaa
     8521 agctcgaagc taatcgatca gctgaaccaa ctcattccgg agggacagcc aaagctttca
     8581 gcgccggact tgaagttcaa ccgttccata ggtgagcatg ccggtaaaac ctttagcgtt
     8641 accggtgaac tcttgtcggc cgaggcgtac aagaagcacg tagcagaagc cctgccgaca
     8701 gcggaagacg agcaaacact caatgaaatc atgaggggca aagagtgggt ttcgcagatt
     8761 cagatgaact gagcaggcga tgcatgccaa tctttagact tgatggcggc cgagcgttaa
     8821 cagaaggaga gagcagtgaa gcggatgtgt ctcgtctgtc agaatattga ttgcaaggct
     8881 cgcggctccg aacagttgat gggcgagctc gagaaacgag tggcggcaaa agggttggaa
     8941 gatgtcgagg tgaaaccgta catgtgcttc ggcgcatgcc aagaggggcc gaacattgtc
     9001 ctttaccccg aaaagagctg gtatgccaac gtgaaggtcg aagacctgga cgagatcgct
     9061 gaacacatcg ccggtggacc ccatgtgaaa cgcttggata ctatcgatcc ttcgctcaaa
     9121 gaattgattt atcaacttct agatacgggg attgtttaac ccatggaagg cattcttttc
     9181 cctcatggtg ctcctgaggg tcgagaagaa ttaaacgact accgcaagcg cggcggctat
     9241 gaggcattag cgcgcgctat caggctgcct gcagaggatg tcgtcggcca agtctctgat
     9301 gccgggctcc gcggcagggg cggcgcggga tttccgacg
//
LOCUS       DASUVS010000245        23009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1849692, whole genome shotgun sequence.
ACCESSION   DASUVS010000245 DASUVS010000000
VERSION     DASUVS010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 23009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23009
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849692"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VF182_13430"
     CDS             complement(<1..914)
                     /locus_tag="VF182_13430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6438134.1"
                     /translation="MENAINQNTLVHVILLAAVCGLVFLLFLDKTPFHDKGEPREALV
                     VRDIVLEGRWLLPLRAGEHIASKPPLFHWFAAGASILRGEMTEASIRFPSALFATLGV
                     FLCYFFARRLYDPKTGLWAGLILATTAVYYIAGIEARVDMTLVFFVTLTLILFFSIYR
                     GFLKHRTWGYVFFATAGTSVTAKGPVSIVLCSLVIATFLLLRKRWDIFRTLLYHPGML
                     LCLGLCVAWYGAALYLGGSEFFNLQFIKENFARYFVYGEGGTGHQKPFYYFVPYLFTL
                     GMPWTLFLPGVIWCYFSDRLRQREDLLFL"
     gene            complement(926..1780)
                     /locus_tag="VF182_13435"
     CDS             complement(926..1780)
                     /locus_tag="VF182_13435"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HEX6438135.1"
                     /translation="MASQLKYRQLRENGWCLRVNLDNWSRELWQAILANIQKQAPERH
                     PQTIKLNLPERHRQTYFLKVYHTASAWSAVKDYFRTSKALRFLRQGMALSDAGFLVPP
                     GIAAGEKRHFGALKAAFVLTVAIDGAPLTTFLEENASGPMALSVVEKRAALRALARQI
                     RRFHDLGFVHGDLLASNLFVCRDVARNFQFVFMDNDRTRRYPKWLPQTLWKRNLVQLN
                     RLPLRSISLQDRIRFFQEYSATKRAADLNRPLLHWLEHKTRERRRECDSVDATISFRR
                     LMRSPRES"
     gene            complement(1894..2661)
                     /locus_tag="VF182_13440"
     CDS             complement(1894..2661)
                     /locus_tag="VF182_13440"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HEX6438136.1"
                     /translation="MLSDERIWGAATDGFNRITPEPHRRFLLRADVADQVQTDIFRGD
                     NHPGQPSRFAGRETLRSIALENGENALIRSYHHGGVLRWITGRIFFSWPPRPFRELAI
                     TEELRRRGVPTVEVYGAGVEGIFGPFYRGWLITRELKGAYDLWTALQNGFVHKWTVEK
                     VFRAVARSLRSLHRQGVYHSDLNLKNILVKSEPAGVKSYIIDFDKAVLFVGDLPDPLA
                     KKNLRRLLRSVSKLDPQRQYLTAAEWDKLLKWYHEAG"
     gene            complement(2709..3806)
                     /gene="waaF"
                     /locus_tag="VF182_13445"
     CDS             complement(2709..3806)
                     /gene="waaF"
                     /locus_tag="VF182_13445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX6438137.1"
                     /translation="MIPADWENVLVVQSSFLGDTVLTLPLLSEIKRHFPAGKLTLLCA
                     PVAAELLGAHALTDEIMIDDKRGRDAGILGLWRKAKILEQKNFTLAISPHKSLRSALL
                     LSLARIPRRIGFRQSKGAFLFHALAERNPLQHEVERNLSILQPLGIDTRTCRRDIELP
                     VAEAGREHARHLLEAMGVDLNKTVIGINAGSVWPTKRWAPELFAQLVTKLKQEIDCDI
                     VLFGAPEDHATVATIQALCGTEVVSLCGKTALGDLPAAVSACDLFISNDSGPMHIAVA
                     CGVPVVAIFCATTPSLGFYPYTSKAVIVQKDLPCRPCSSHGGRRCPLGTEDCIKLIRP
                     DHVLQAAMQMLRINEPLPLASTTHEPQYLDL"
     gene            complement(3810..3977)
                     /locus_tag="VF182_13450"
     CDS             complement(3810..3977)
                     /locus_tag="VF182_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX6438138.1"
                     /translation="MAISKDLLDILACPKCKGDIHLNESGDGLICDACRLMYPIKDDI
                     PVMLIDEAERI"
     gene            complement(4096..4956)
                     /locus_tag="VF182_13455"
     CDS             complement(4096..4956)
                     /locus_tag="VF182_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438139.1"
                     /translation="MLDYTDEDVGNIVSLEHVNLQVPDQAMATVFYIVGLGLTRDPYL
                     NVGLGNMWANIGEQQFHLPTRSAQKISGYIDLVLPDLDALEERLSSIKPALEGTQFSW
                     SSNQDYVAVTCPWGNRFRCYKSGPPYGDMAQGLAAVDFNVPKGTAAAIAGFYQNVLAA
                     PATVETDSGHTVAHVDIGRNQSLRFRESKETIAPYDGHHIAIYVANFSRAYHFLKSRQ
                     LISEDVRNHQFRFKSIIEPDSGRIVFLLEHEVRSLHHPMFHRPFVNRDPAQSQRNYRR
                     GRDALIPFRP"
     gene            complement(5004..5945)
                     /locus_tag="VF182_13460"
     CDS             complement(5004..5945)
                     /locus_tag="VF182_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017662991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6438140.1"
                     /translation="MKVLITGVAGFVGSHLAEKLLSENYDVVGVDKFLDNYPRRFKQK
                     NLEPSLKHRNFKFVEEDLLGLNLRELLKDVSYVFHLAAQPGVRSSWGDEFIQYSQNNI
                     MATQVLLEAGKESGVKKIVYASSSSVYGDTDDLPMREEGNTRPVSPYGVSKLAAEHLC
                     YLYWKAYGLPTVSLRFFTVYGPRQRPDMFFHIFMDRLRKNQEVPLYDDGEQTRDFTYC
                     GDIVDGLLGAAVYPGAGEVFNLGGGTESSVVNAIHIVEKISGRQAKLKTFERQKGDVR
                     HTRARLDSAAEKLRYRPKISLEEGLRQQWHWIQTLDG"
     gene            complement(6049..7152)
                     /gene="lpxK"
                     /locus_tag="VF182_13465"
     CDS             complement(6049..7152)
                     /gene="lpxK"
                     /locus_tag="VF182_13465"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX6438141.1"
                     /translation="METLGIQRVWQRTGTSGKLLWVLLLPASWAYRFAVAIRNLSYRW
                     RCARANALDCPVISIGNLSVGGTGKTPATVWLAQRLMQRGFTVGILSRGYKRKGSGPI
                     ILSGDNGMSSATLDGSEALAAGDEPVMMARIYGLTVSVAKNRYRAGLELLAKKNMDVL
                     ILDDGFQHRKLKRNFDLVLLGEDVPGSLLPCGPFREPTRSLRRADSFLVTGVAERWQK
                     WIEANGGRPSFQAMLQPQALIGFEFDQRKEYPLTLLYRSKILAVSGIAKPAKLYQTIH
                     EYDGELVDTLEYPDHHEYSAADWQQINRIARDVDLIVTTEKDIPKLMRFPFARGKLLA
                     LRVSMAVAQEQALLDLVIGAIRDHGRHQNEGKT"
     gene            complement(7160..8479)
                     /locus_tag="VF182_13470"
     CDS             complement(7160..8479)
                     /locus_tag="VF182_13470"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEX6438142.1"
                     /translation="MAFVVYNVLITLAFLIALPVLPMLLLGGERFRAGLGQRLGWYPR
                     HSVVPFTAPRPLWIHAASVGEVRSVRVLSEQLKRRFPGRAIILSTLTHTGNLTARQNP
                     AIDRVIFLPLDLWWTVRRALAVFDPAVVIFIETEIWPNFLHQSHRKGLPTILLSGRVS
                     EKAFNHYCRLAWFFKGVFSLLRACGMQSEIDRQRIIRLGATPNRVSITGNLKHAAVLN
                     DGVAAGCMISEHRSPGSWIWIVGSSHRGEEEIVCAAFKILKQRFPRLRMILAPRHPQR
                     FAEVEKLLVDGGVSFEKKTSLGNRREFRQDVLFLDTIGDLERFYADADLAFVGGSLVD
                     AGGHNLLEPARLGKPVLFGPYTSTVKLVARQLKQNGGGFEVSDAEDLAREITGLLTDP
                     EKRRLAGEKAYEVAAGDNAVIGRSLNIVATYLPASAANVVSSSAPTR"
     gene            complement(8494..10248)
                     /locus_tag="VF182_13475"
     CDS             complement(8494..10248)
                     /locus_tag="VF182_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX6438143.1"
                     /translation="MKTYRRLLPFLKPYIWPYFVLGVICMLGYSATDGVIPFLVRGVF
                     DDLFQKRDPNMLRYLPLAIIIVFSARGLLNFGQNYFSDYVGLRIINDVRNRLNSHLQY
                     LSLAFFYRHATGTLLSRVGNDVTLLRSVLTDALAALVRDSLSVIVLIAVAFYQDWVLA
                     SIAFFVFPAAVLPVMRMSRKIKRFTKRGQISTGNLTALLQESVQGNRIVKAFGMEEYE
                     ITRFTAENWRLFRQSMRASRIKAVVTPTMEFLASFGIAGVVWYGGASVIGGGRTQGEF
                     MAFMAAMMLMYQPFKSLARTYTAVQQGIAGAERVFEILDTHSDVQERPDSRPAPRFAE
                     SIEFHDVSFGYGEKLVLKNISLRIHAGEMVALVGVSGVGKSTMADLIPRFYDVTSGAI
                     TMDNTDIRELTLRTLRHQIGLVSQHTFLFNDTVRNNISYGDPERSMDDIIAAARAAYA
                     HDFIMALPKGYDTDIGEMGLQLSGGQRQRLAIARALLKDAPILILDEATSALDAESER
                     LVQNALDNLMTRRTTVVVAHRLSTIRRADRIVVLVGGKIAEQGSHEQLMARKQEYSRL
                     YSMQLLEDAEPAEPKMMH"
     gene            complement(10266..11411)
                     /gene="lpxB"
                     /locus_tag="VF182_13480"
     CDS             complement(10266..11411)
                     /gene="lpxB"
                     /locus_tag="VF182_13480"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX6438144.1"
                     /translation="MDRGKEIMLVVGEASGDMHGASLVRALLKRDPHLRFFGVAGDQL
                     QRTNFEAVFTVAELTGMGLLELAGNIKNIVAAYRLLRRMLRTRRPRLLILIDFPELNL
                     RLARLAKSLGIPVLYYVSPQVWAWRRGRIRQIAQVVDKMAVVFPFEVEFYERHQMPVE
                     FVGHPLLDVVGVSQGRDATLREIGLDKEKPVIALLPGSRRKEIAYHLPVMVAAAAELK
                     RVRQAQFFCARASTLDRAVLQKELDRAGFAIPIVDSGRYNAVNAADLVWAASGTATLE
                     TALLEKPMIVLYRVSWVTYWLARLLVKIKYIGIANIIAGKAIVPELVQSDLSPERLVH
                     ESQAILNDDDARAKMIANLSKLRDQLGSPGAAYRVAELAVSMMAVKA"
     gene            complement(11439..12371)
                     /locus_tag="VF182_13485"
     CDS             complement(11439..12371)
                     /locus_tag="VF182_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6438145.1"
                     /translation="MHPAQKILAAVIGVGYLGKFHAEKYAASEKADLVAVVDQDSARA
                     AEIGSGVGAAGLTDYRELFGRVQCVSIAVPTRVHHQVARDFITAGIDVLVEKPLAATL
                     DEAKELVELAESQHCILQVGHLERFNPAIRRLEGVIKEPKFVECHRLAPFVERGTDVD
                     VVLDLMIHDIDVIASLVRSPVIRAEAVGVPVLTETPDIANARLTFANGCIANVTASRV
                     SIKRERKVRFFQPDAYISIDYDQRRAQIIYKPAPGAGWLDVRSENIEFKEGDALADEI
                     DSFLDCVRARTAPLVSGIDGLNALEIASLIRAQL"
     gene            complement(12384..13205)
                     /gene="lpxI"
                     /locus_tag="VF182_13490"
     CDS             complement(12384..13205)
                     /gene="lpxI"
                     /locus_tag="VF182_13490"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707637.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEX6438146.1"
                     /translation="MTSLHDKRLGLIAGNGRFPYIFAQEAKREGYSIVAVAHRDETLA
                     EIEKVVDELTWVYVGQLGKIIRTFQRARVTQAVMAGGIRKVRLFGNFRPDLRGASFLA
                     RVKSRDDDGLLRGIANELEKDGIAILESTLCVPQIITTAGVLTRHAPSKRQWEDIRVG
                     IRIAKEIGRLGIGQTVVVKNGMVIAVEAVEGTDEAIKRGGNLARSGCVVIKVSKPNQD
                     LRFDVPAVGLETIKILRHAGAVALALEAEKTLLLEKDALLAEADAGGIAVVGVAV"
     gene            complement(13241..14017)
                     /gene="lpxA"
                     /locus_tag="VF182_13495"
     CDS             complement(13241..14017)
                     /gene="lpxA"
                     /locus_tag="VF182_13495"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX6438147.1"
                     /translation="MSRIHQTALINCQAEIDSDVEIGPYSVIGAGVRIGRATKVASHV
                     VIEGRTSLGQGNTIYQFATIGSRPQDLKYRGEASELIIGNHNTIREYVSLNPGTAGGG
                     MVTRVGDHNLLMMHCHIAHDCLIGNHNIIANGATLGGHVVVEDYVIVGGLVGIHQFAR
                     IGSGAILGAGSMVSKDVPPFCNTTGDRARLRGLNLEGLKRRGFTPAAIDALKKAYRII
                     FQSKLKTKDALEKIRREVSPTAEIDILLAFIAQSQRGICR"
     gene            complement(14038..14475)
                     /gene="fabZ"
                     /locus_tag="VF182_13500"
     CDS             complement(14038..14475)
                     /gene="fabZ"
                     /locus_tag="VF182_13500"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX6438148.1"
                     /translation="MLEVKDILKYLPHRYPFLLVDKILEIDGEEKIVAIKNVSFNENF
                     FQGHFPNRPVMPGVLICEAMAQVGAIFAHNARGGMDDNKVFVLTGLDNVKFKRPVEPG
                     DQLRMELTCLKRRGSFWKMHGSALVDGKLVAEAEISAMEIDMP"
     gene            complement(14480..15544)
                     /gene="lpxD"
                     /locus_tag="VF182_13505"
     CDS             complement(14480..15544)
                     /gene="lpxD"
                     /locus_tag="VF182_13505"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX6438149.1"
                     /translation="MPSRQRTLRDLAEYVGGRVIGDGSETIFRVSPIEEAGPGDITFL
                     ANPRYSRFISGCKASAIIVGPGLVGSELARSGQDFLEVAQPYVAFAKILQLFAPAAEY
                     QREIAPSAHIDPSSKLGHDVSVYPNVYVGQCAQVGDRTVLLPGVFLGDRVAIGSDCVL
                     HPNVVVRDGCRLGNRVILHAGVIVGSDGFGYAGSGEARIKIPQVGMVEIEDDVEIGAN
                     TTIDRATLGRTLIGRGAKIDNLVQIAHNVNVGANSVIAAQAGIAGSTKLGQNVTLAGQ
                     VGVVNHIEIGDGAIIGPQSGVPQSVPAGALLSGGIGVAPHHEWLKVMTLLPQLPKLWT
                     LVRRLEKQVAQLANRQPKEK"
     gene            complement(15547..16053)
                     /locus_tag="VF182_13510"
     CDS             complement(15547..16053)
                     /locus_tag="VF182_13510"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX6438150.1"
                     /translation="MKYWLVLFLTLLWAPAVGAQDRVKIGFIDIQRAISDSNAGKRAK
                     ERFQAQVKKAEAELLKEKNELERLKADLDKKGPLMKEDERRNLEGDLQRRYVNYQRTM
                     TDQQQELRQKEGALTGDILKELEKIVNEIGKSDKFTLILERSQILYSDQGIDVTNKVI
                     EVFNSRNK"
     gene            complement(16099..18630)
                     /gene="bamA"
                     /locus_tag="VF182_13515"
     CDS             complement(16099..18630)
                     /gene="bamA"
                     /locus_tag="VF182_13515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX6438151.1"
                     /translation="MASCSRSLTAADLTLQNEANLLEIRQSHPSKEVLPLARMKSQVT
                     ITEIIRSGLFCFLLLSATWASALRAQEKITLKDVRITGNVRVEEDGIRLHIKSRAGAE
                     FDPALVEQDVKGIFRMGFFDDVEAQLSPENVLIYSVKEKPYVREVRIQGASQVGRDKI
                     EAALGVTPRTVLDRSKVAEGIEKVRKLYGEQGYGNAQVDYAVSVESNNQAVLVLDIVE
                     GNRLLIKKISFEGNRTFSESELKGLMATKEEWLFSFITNRGVLDRDILTNDVAILGNH
                     YIDHGYIDHKIDEPVVLRGRDGLELVIRVDEGPQYRVGKVEIGGDLIQGGQEMLKQIK
                     LTEGQIFRGSRLRDDISTLTDLYSNKGFAFAQIDPVTKVNANEKKVDVALVIAKGPPV
                     YFNRVLVAGNTKTRDKVVRRELLASEQELYSSSKITQSRNALQRTGYFEDVQLTTKKT
                     DQPDTVDLLVDVKEGPTGTFNLGAGFSSGDGFIFNAGVAEKNLFGRGQGLNGSFAIGS
                     SRQDFIVGFTDPYFNDSQMSLGIDAFNTEREFHDFDERKLGFGINTSYPLKDVSVPFY
                     GRLRKQPSLGSDQLASNPPPSLWDYMRGGVGYELTRETINDVESTAPGSIQDEKGTSL
                     TSAMSPSISYDSRDHFFSPTQGTKSAFSVKFAGLGGDNRFIKSDLSGRWHYPLLKDPN
                     WGGAYVLALGGSLGYGVGLAERDNGKKDLPLFERYFLGGINSVRGFAERSLGPREPAN
                     CEGPNGPCTDTEVVGGDRAMVLSAELMFPIMEQFGLRGVAFFDMGNSFGDSDKFSFTD
                     LRRSVGFGARWMSPFGPLRVELGFPLNKQSGDETSVLGFTLGSQP"
     gene            complement(18596..19285)
                     /locus_tag="VF182_13520"
     CDS             complement(18596..19285)
                     /locus_tag="VF182_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6438152.1"
                     /translation="MSSLLSAREIDKSYSEGGREIVVLRQLSLELETAERLAIVGESG
                     VGKSTLLHILGTLDRPTGGRIIYSGKELPLNDEGALSAFRNREIGFVFQFHYLLPDFS
                     ALENVMLPALIQGMSPAQAREQAEDLLESVGLKERMSHRPGKLSGGEQQRVSVARSVI
                     LQPKLILADEPTGSLDFRIGQEVHDLLFQLNEQRGVALIIATHNREFAEKIGRQLELR
                     DGQLFPVTHCR"
     gene            complement(19278..20525)
                     /locus_tag="VF182_13525"
     CDS             complement(19278..20525)
                     /locus_tag="VF182_13525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEX6438153.1"
                     /translation="MNYELFIGLRYLRARRQETFISLITVISVLGVMIGVMTLNVVMA
                     VMTGFEETLRDRLLGINAHIALLKSGDPLSEYKELLQRVVKEEGVVAASPAIYGQVML
                     TSGSRVSGVVVRGVDPDRVNAVVDLQRYMKEGSIAGLKKQNPVQVQDRTIMLPGVIMG
                     ERLAAQLGLFVGSPVQVVSPLGSPTAIGVIPKVRRFVVTGLLNTGMSEIDSTLVFMGL
                     ADAQKFFELGDAVSNIEIRVDDVNQSREIADRIQRQLGFPYLTEDWTRLWPNLFSALQ
                     LEKTVYFLVLLLMVLIGAFNIISTLVMVVMEKRKDIAILMSMGATRASIRKIFLLKGL
                     IIGVVGTTLGLLLGLLVCTLIAQYRFELPKDVFLISTVPVRIYFSNFMIVTLSSFIVC
                     LLASIYPARQAAKLDPVEIIRYE"
     gene            complement(20547..22052)
                     /gene="lysS"
                     /locus_tag="VF182_13530"
     CDS             complement(20547..22052)
                     /gene="lysS"
                     /locus_tag="VF182_13530"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX6438154.1"
                     /translation="MPTFEKELVEETGEQIEVRRQKLAKLREAGHKIYPNDFRPNHTT
                     VEVVSKFNECREEDLPGVANDLSLAGRIIGIRKFGKASFFHLQDRRGRLQIYARKDRL
                     GDDGYALFQSFDVGDIVGVSGHLFRTKTKELTLEAESVRLLAKCLRPLPEKWHGLADV
                     EARYRQRYVDLMVNPEVRDVFERRSRIVRLVRKFFEERDFLEVETPMMQAIPGGAAAR
                     PFTTHHNALDLELFLRIAPELFLKRLVVGGIERVFELNRNFRNEGISVRHNPEFTMLE
                     FYQAYATFEDLMHLTEELFVFLANQVAGTLEVPYGEHVIDLKPPWRRMTIADAIALYG
                     NVETNEIANLGGLQSFAKSRGLQVDVDAPHGTLLVEVFEEVAEAKIIQPTFISGYPIE
                     VSPLARKNDNDPALVDRFELFIAGRELANAFSELNDPADQRQRFLEQMKARASGDDTA
                     NPIDEDYLRALEYGMPPAAGEGIGIDRLVMFLTNSASIRDVILFPLLRPQR"
     gene            complement(22116..>23009)
                     /gene="metF"
                     /locus_tag="VF182_13535"
     CDS             complement(22116..>23009)
                     /gene="metF"
                     /locus_tag="VF182_13535"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEX6438155.1"
                     /translation="GYGIHQEYSLVKLADLYAQPGLTLSVEFFPPKTDKGEEALFGEI
                     EFIKSLRPAFCSVTYGAGGSTREKTVDLVDRIHRECGLETMCHLTVVGQSKDDARGVL
                     RRLQQKGLENVLALGGDPPAGMPNWQPHPDGFQHAVELVKEAISFGCFSVAVAGFPEI
                     HPRAKSRTSDLGYLKEKIDAGAQAIITQLFFDNAQYYRYVEDVRKLGIQVPIIPGVLP
                     IVSAPQIRRFTALCRARIPPQLEQALNKVENDDNAATQLGIEYATRQCEALIQFGVSG
                     LHFYSLNKSHSVSAICKNLGI"
BASE COUNT         5464 a         6514 c         5883 g         5148 t
ORIGIN      
        1 aggaacagca aatcctctct ctggcgcaaa cgatccgaaa aatagcacca aatcacacca
       61 ggcaaaaaca aggtccaagg cattcccaac gtgaaaaggt aaggaacgaa atagtagaac
      121 ggtttctggt gtcccgttcc accttcacca tagacgaaat aacgcgcgaa gttttcttta
      181 atgaactgca ggttgaaaaa ttcgctgcct cctaggtaaa gcgccgcacc gtaccacgcc
      241 acgcacaggc ccaaacagag gagcattccc ggatgataaa gcagggtccg aaagatatcc
      301 catctttttc gcagcagcag aaaagtagca atcacaagac tgcagagaac aatgctgacc
      361 ggcccttttg cggtaacgct ggtcccggca gtcgcgaaaa atacgtaccc ccacgttcga
      421 tgcttgagaa agccgcgata aattgaaaag aacaaaatca gagtcagtgt cacaaaaaac
      481 accagggtca tgtccacccg agcttcgatg cccgcgatgt agtacacggc ggttgtcgcg
      541 agaatcaaac ccgcccaaag cccggttttg ggatcataca gacgccgggc gaaaaaataa
      601 caaagaaata cgccaagagt ggcgaagagc gctgagggaa agcggatgct cgcctcggtc
      661 atttcgccgc gaaggattga agctccggcc gcaaaccaat ggaacaacgg cggcttcgaa
      721 gcgatgtgtt cgccggccct gagaggaagc aaccaacgtc cttcgaggac aatgtcgcgc
      781 accacgagag cctcgcgcgg ctctcccttg tcatgaaacg gagtcttatc gaggaaaagc
      841 aagaatacca acccgcaaac cgccgccaaa aggatcacgt ggactagggt attttggttg
      901 atcgcgtttt ccacgtcttt gtcggttaac tttctcttgg agaacgcatc aagcgccgga
      961 agctaatcgt cgcatccacc gaatcgcatt ctctgcggcg ctcgcgggtc ttgtgttcca
     1021 accaatgcag taaggggcgg ttgagatcag ctgcccgctt cgtggccgag tattcttgaa
     1081 agaacctgat tcgatcttgt aaggagatgg atcggagagg caagcggttg agctgcacta
     1141 aattgcgttt ccatagagtt tgcggcagcc atttaggata tcgccgggtg cgatcgttat
     1201 ccataaaaac aaactggaaa tttcttgcaa catcccggca gacaaaaagg tttgaagcca
     1261 gcaagtcacc gtgcacgaag ccgagatcgt gaaagcgccg gatttgccgc gccaaagctc
     1321 gcaaggccgc tctcttctcg accaccgaaa gcgccatggg gccactggca ttctcctcta
     1381 gaaaggttgt caagggcgcg ccgtcgatag cgacggtgag gacgaacgcc gctttcaacg
     1441 cgccgaagtg acgtttttca cccgctgcaa ttcctggcgg aacgaggaat ccggcgtcgg
     1501 aaagcgccat gccttgtctg agaaaacgca gcgccttaga agtgcgaaaa tagtctttca
     1561 ccgcgctcca cgccgatgct gtatgataaa ctttcaagaa gtaagtctgc ctgtgtcttt
     1621 caggcaagtt caacttgatg gtttgcggat gacgctcagg agcctgtttc tggatgttcg
     1681 ccaagatagc ttgccagagc tcccgggacc aattatccaa attgacgcgg aggcaccagc
     1741 cattttccct gagctgccga tatttcagct gcgaggccaa cgtctcacct ttactgcgcg
     1801 cgctttgccc ggttcaatat ctcggcctga atgtcattgg agccggtgga gccatacagc
     1861 acgatcggaa tgccgcccac tactacgctc atttcatccg gcctcatgat accatttcaa
     1921 aagtttatcc cattccgccg cggtcaagta ttggcgctgc ggatcgagct tcgatactga
     1981 acgtaaaagc cggcgtagat ttttttttgc aagtggatcc ggcaggtctc caacgaacaa
     2041 tactgccttg tcaaaatcta taatataact cttcaccccg gccggctcgg acttcaccaa
     2101 tatgttctta agattcaaat cactatgata aacaccttgg cgatgcaggg agcgaaggct
     2161 ccttgccacc gctcgaaaaa ccttctcaac cgtccatttg tggacaaagc cgttttgcaa
     2221 ggctgtccac agatcatagg cgcccttcaa ctctcgggtg atgagccagc cgcggtagaa
     2281 agggccgaag ataccctcta cacctgcgcc atagacctcg actgttggaa caccgcggcg
     2341 acgcaactcc tcggtaatcg ctaattcccg gaacggccga gggggccagg agaaaaatat
     2401 ccggccggtg atccagcgga gaaccccgcc atgatggtag ctgcgaatca gagcgttttc
     2461 gccgttttct aacgcaatgc ttcgtagtgt ctcccttcct gcaaaccggc tcggctgacc
     2521 cggatgatta tctccccgga atatgtccgt ctgaacttga tccgccacgt cggcgcgcag
     2581 cagaaagcgg cgatgcggct cgggcgttat tctattgaag ccgtcggtgg ctgcgcccca
     2641 aattcgttcg tcgcttaaca cgttaaacct aggccagctc tggctcgagc cagcgatatg
     2701 aggggctgtt acaaatcgag atactgaggc tcgtgagtgg tcgaagctaa cggcagtggc
     2761 tcattgattc gcaacatttg catggctgct tgcaagacat ggtctggccg aatgagcttg
     2821 atgcaatctt ccgttcccaa cggacagcgt ctacctccgt gagaactaca gggacggcac
     2881 gggaggtcct tttgtacgat cacggccttt gaagtgtatg gataaaaacc cagagacggt
     2941 gtggtagcgc agaatatcgc aaccaccgga acaccgcagg caacggcaat atgcatcggg
     3001 ccgctatcgt tactgataaa cagatcacat gcactgacgg cggccggcag atcgccgaga
     3061 gcagttttgc cgcacaggct taccacctcg gtgccacaga gcgcctggat tgttgccacg
     3121 gttgcatgat cctccggagc gccgaaaaga acaatatcgc aatcaatttc ctgcttaagc
     3181 ttcgtaacca attgcgcgaa taactcgggc gcccaacgtt tagtgggcca aacggagccg
     3241 gcattgatac ctatgacagt cttgtttaga tcaacgccca tggcctccag taagtggcgc
     3301 gcgtgttctc tgccggcctc ggccacaggg agttcgatgt cacggcgaca tgtcctagta
     3361 tcaatgccta gaggctgcag gatcgaaaga ttcctctcga cttcatgctg cagcggattt
     3421 ctttcggcca aggcgtggaa caagaatgct cccttacttt ggcggaagcc gatacgccgg
     3481 ggaattctgg ccagggacaa caacaaagcg ctgcgcagtg acttgtgcgg agaaatggcc
     3541 agcgtgaaat ttttttgttc gagaatcttg gctttacgcc ataagccaag gataccggca
     3601 tcccggcctc gtttatcatc gatcatgatc tcatcagtga gcgcatgcgc ccccaacaac
     3661 tccgccgcca caggggcaca aagcaaagtc agcttgccgg cgggaaagtg ccgcttgatt
     3721 tctgaaagca atggcaacgt cagaaccgtg tcgcctagaa agctggattg aaccaccagg
     3781 acgttttccc aatcggccgg aatcatcgtt caaatgcgct cggcctcgtc aatgagcata
     3841 acggggatat cgtcctttat gggatacatg aggcggcagg catcacagat aagaccatca
     3901 ccgctctcgt ttagatgaat atcgcccttg catttcggac atgccaatat atcgagcaag
     3961 tctttgctaa tcgccatatt tcctcctaac tggctattga tgggcgcgag gtttccgatg
     4021 cccattcaag atcggttcgc cgggtcaata gctggaacgc atctcgacat ccgccacagt
     4081 ctatactccc gtcgatcacg ggcgaaacgg tataagggca tcccggccgc ggcgataatt
     4141 gcgctgactc tgcgccggat ctcggttgac gaaaggcctg tgaaacatgg ggtgatgaag
     4201 acttctaact tcatgctcta aaagaaatac aatcctgccg ctgtcgggtt caatgataga
     4261 tttgaaccta aactggtgat tgcggacatc ttccgagatc aattgacggc ttttaagaaa
     4321 atgatatgcc cgtgaaaagt tcgccacata gatggcgata tggtgaccat cataaggagc
     4381 gatggtttct ttcgattccc gaaaacgaag cgattgatta cgcccaatat cgacatgcgc
     4441 cacagtgtgg cccgaatctg tttcgacagt agcgggcgcc gcaagcacgt tttggtagaa
     4501 accggcgatt gccgccgcag ttcctttggg cacattgaaa tcaacagcgg caagtccctg
     4561 cgccatatcg ccgtagggag gtccgctctt ataacagcgg aagcgattgc cccagggaca
     4621 agtgacggca acataatcct ggttgctgga ccatgaaaac tgcgtgcctt ccagcgctgg
     4681 ctttatcgac gaaagccgtt cctccaaggc gtctagatcc ggcaacacca gatcgatata
     4741 gcccgagatt ttttgcgcag atcgggttgg tagatggaac tgctgctcgc ctatgttagc
     4801 ccacatattg ccgaggccga cgttcagata aggatcacgg gttaacccga ggccgacaat
     4861 ataaaataca gtcgccatag cctgatcggg cacttggaga ttgacgtgct ccagcgatac
     4921 gatattgcct acgtcttcgt cagtataatc aagcatacgg ctctcctccg ggcccggcga
     4981 cttggttgcc attcagtatc ttatcagccg tcaagcgtct gaatccagtg ccactgctga
     5041 cgtaagcctt cttccaaact gatcttgggc cgatagcgca acttctcagc ggccgaatcc
     5101 aggcgcgccc gggtgtgccg tacgtcccct ttttgccgtt caaaggtctt tagctttgcc
     5161 tgacggcccg aaatcttctc gacaatgtgg atcgcattga caacggagct ctcggtaccg
     5221 ccgccgaggt tgaacacttc gcctgccccc ggatagaccg ctgcccctaa tagtccatcg
     5281 acgatatcgc cacagtatgt aaaatcccgg gtttgctcgc cgtcgtcata gagcggcact
     5341 tcctgattct tgcgcagacg atccatgaaa atgtgaaaga acatgtccgg cctttgcctc
     5401 ggcccataaa cagtaaagaa ccgcagagat acggtcggca agccgtacgc tttccagtag
     5461 agatagcaaa ggtgttccgc cgccagcttg ctgacgccgt aaggcgacac cggccgagta
     5521 ttaccctcct cgcgcatcgg gaggtcgtca gtatcgccgt aaaccgagga cgacgaagca
     5581 taaacaatct ttttgacgcc ggattcttta ccggcttcca aaagtacttg agtcgccata
     5641 atgttgttct gactatactg gatgaattca tcaccccaac tcgagcgaac tccgggttgc
     5701 gctgccaaat gaaagacgta ggatacgtcc ttcagaagct cccgcagatt tagtcccagg
     5761 agatcttcct cgacaaactt gaaattccgg tgtttcagag atggttcgag gttcttttgt
     5821 ttgaatcgac gaggatagtt atccagaaac ttgtcgacgc cgacaacgtc atagttttcg
     5881 ctaagcagtt tctccgccaa atgggagccg acgaacccgg caacgccggt gatcaggact
     5941 ttcatactaa ccctcctgga agctaaatga ctcgatgtct gcggccaact cccgtcctat
     6001 tcgtcactcc ctgccaataa agcactcaca gatagtgctg gaatgcgatc aggttttgcc
     6061 ttcattctga tgacggccgt ggtcacggat cgcgccgatg accaggtcga gtaaagcttg
     6121 ttcctgcgcc accgccatcg aaacccgcaa tgccaacaat ttaccccggg caaaagggaa
     6181 gcgcatgagc ttcggaatat ccttttcggt ggtcacgata agatcgacat cgcgcgcgat
     6241 gcgattgatc tgttgccagt ctgcggccga atattcgtga tggtccggat attccagagt
     6301 atcgacgagc tcgccatcat actcatggat cgtttgataa agtttcgccg gctttgcgat
     6361 tccggaaacc gccaggatct tactgcgata cagcagggtc agaggatact ctttgcgctg
     6421 gtcgaattcg aacccaatca gcgcttgcgg ctgaagcatt gcttggaaag agggacgccc
     6481 tccgttggct tcaatccatt tctgccagcg ctcggcaacg cctgtgacta aaaaactgtc
     6541 ggcccgcctc agagagcggg tcggttcacg gaacgggccg caaggcaaga gtgatcccgg
     6601 cacgtcctcg cctagcagca ccagatcaaa attccgcttt aatttcctgt gctggaaacc
     6661 atcatccagg ataaggacgt ccatattttt ctttgccagc agctccaatc cagcccgata
     6721 tcgattcttg gccacactta cagtcaatcc ataaatgcgc gccatcatca ccggctcatc
     6781 gccggcagcg agcgcttccg atccgtcaag ggtggctgaa gacatgccgt tatcgcccga
     6841 cagaatgatc ggtcccgatc ccttccgctt gtagccgcgg ctgagaatgc caaccgtaaa
     6901 tccgcgttgc atcaagcgtt gcgccaacca taccgtcgcc ggtgtttttc cggttccacc
     6961 caccgacaag ttgccgatgc tgataaccgg gcagtcgagg gcgttagccc gagcgcaccg
     7021 ccatcgatat gacagattgc ggattgctac ggcgaagcga taagcccatg aagccggcaa
     7081 aagcaacacc catagaagtt tgcctgatgt tcctgttcgt tgccaaacgc gctgaatgcc
     7141 cagggtttcc atcaagactc taacgtgtgg gagcggatga cgaaactacg ttggcggccg
     7201 aagcgggcag ataggttgcg acaatgttca agctacgacc gatcactgcg ttgtcgccgg
     7261 cagcgacttc atacgccttc tcaccagcga gccgtcgctt ttccggatcc gtgagcaggc
     7321 cggtaatttc acgcgccaga tcttcggcgt cgcttacttc aaagccgcca ccattttgct
     7381 tcagctggcg cgccaccaat ttaacagtcg aagtataggg accaaagaga accggtttgc
     7441 ccaagcgcgc cggttccaga agattatgcc cgcctgcatc tacaaggctg ccgccgacaa
     7501 acgctaaatc ggcatccgca tagaaacgtt ccaggtcgcc gatggtgtcc aagaagagaa
     7561 cgtcctgccg gaactcacgc cggtttccca ggctggtttt cttttcaaac gagactccgc
     7621 cgtcgaccaa cagtttttcg acttcggcaa aacgttgcgg atggcgcggc gcgagaatca
     7681 tgcgcaggcg cgggaatcgc tgcttcagga ttttgaacgc cgcacagacg atctcctcct
     7741 cgcctcgatg cgagctccct acgatccaga tccagctgcc gggcgacctg tgctcgctga
     7801 tcatgcatcc cgcagcaact ccgtcattca gcacagcggc atgcttcagg ttcccagtga
     7861 ttgaaacccg gttgggcgtt gcgccgagcc tgatgattcg ctgccggtcg atttccgact
     7921 gcatgccgca ggctctcaat aagctaaata ctcctttgaa gaaccaagcc aaccggcagt
     7981 aatgattaaa tgccttctcc gaaacgcgcc cgctcagaag gatcgtcggt aagcctttgc
     8041 gatgagattg atgcaaaaaa ttgggccaga tttcagtctc gataaaaatc accacagctg
     8101 gatcaaaaac agccagcgct cgacgcaccg tccaccacag atctaaaggc aaaaaaataa
     8161 ctcgatcgat cgccggattt tgtcgcgcgg tgagatttcc tgtatgcgtc agagtggaca
     8221 ggatgatggc ccgaccaggg aagcgccgct tgagttgttc gctcagaacg cgcaccgacc
     8281 tgacctcgcc gaccgaagcc gcatggatcc atagcggtcg cggcgccgtg aatggaacca
     8341 cactgtgcct cggataccat cccagtctct gtccaagacc agcccggaag cgttctcctc
     8401 ctaagaggag cattgggaga accggcaggg cgatcaagaa agccagagtg ataagcacat
     8461 tataaacgac aaaagccatc tctcgacgat agttcagtgc atcatctttg gttcagccgg
     8521 ttccgcatct tcgagcaact gcatactata gagccggctg tactcctgct tgcgagccat
     8581 caattgttcg tgactacctt gttccgctat ttttccacca accaagacca cgatgcggtc
     8641 ggcccggcga atcgtcgaca gccggtgagc gaccactacg gtggtccgtc gggtcatgag
     8701 attgtcgaga gcattttgca ccaatctctc cgattcggca tcgagagccg aggtcgcctc
     8761 gtcgagaatg agaataggcg catcctttag cagtgcccgc gcgatggcaa gccgctgcct
     8821 ctgcccgccg gaaagttgca gccccatttc gccgatatcc gtatcatagc ccttgggcag
     8881 cgccatgatg aaatcatggg cataggccgc cctagcggcg gcaataatat catccatgct
     8941 tcgctccgga tcgccatagc tgatgttgtt gcgcaccgtg tcgttgaata aaaaggtatg
     9001 ctgactcacc aggccgattt ggtgccgcaa cgttcgcaac gtaagctctc ggatatcggt
     9061 gttgtccata gtaatagcgc cggaggtcac atcgtagaac cgcgggatca gatcggccat
     9121 ggtgcttttg ccgacgccgc taactcctac caacgcgacc atttcaccgg cgtgaatacg
     9181 caggctgata ttctttaaga cgagcttttc accgtaaccg aagctcacgt catgaaattc
     9241 gatggattcc gcgaatctcg gagccgggcg cgagtccggg cgttcctgaa cgtcggaatg
     9301 cgtatccaag atttcgaaaa ctctttcggc tccggctatg ccttgctgaa ccgccgtgta
     9361 ggttctggcg agagatttaa acggttgata catgagcatc atcgcggcca taaaggccat
     9421 gaattcgcct tgcgttcgcc cgccgccgat cacgctcgcg cctccatacc agacaacccc
     9481 ggcaatccca aaggatgcca gaaattccat ggtgggggtg accaccgcct tgattcggct
     9541 tgcccgcatg gattgccgga aaaggcgcca gttctccgcc gtaaatcgcg tgatctcata
     9601 ctcttccatg ccgaacgcct tgacgatgcg attgccttga acgctttcct gcagtaacgc
     9661 ggttagattc cccgtgctga tctggccgcg cttggtaaag cgtttgattt ttctgctcat
     9721 gcgcatcacc ggcaggactg cggccggaaa tacaaaaaat gcgatcgagg ctaaaaccca
     9781 gtcctggtag aacgcaacgg ctatcagaac gattacggag agactgtctc gcaccagcgc
     9841 ggccagcgca tcggtaagca ccgagcgcaa gagagtgacg tcgttgccga cccgggagag
     9901 aagtgtccct gtggcgtgac ggtaaaaaaa agccaacgac agatactgca gatgtgaatt
     9961 gagccggttg cgcacatcgt tgataatgcg caagccgaca taatcactga aataattctg
    10021 gccgaagttg agcagaccgc gcgcgctaaa gacaatgatg atggctagtg gaagatagcg
    10081 cagcatgttc ggatcccttt tttgaaaaag atcatcgaac acaccccgca ccaggaatgg
    10141 aatcacgcca tcggtggcgc tatagccgag catgcaaatc acgcccaaga cgaagtaggg
    10201 ccaaatgtac ggttttaaaa agggcaataa tcgtcgatag gttttcacgg agtttcaatc
    10261 caaggctagg ctttgaccgc catcatagag acggcgagct cggcgacgcg ataggccgcg
    10321 ccgggggaac cgagttggtc cctgagctta ctcaggtttg cgatcatctt cgcgcgggca
    10381 tcgtcatcat tgagtatcgc ctgactctcg tgcacgagcc gttcgggcga aagatccgat
    10441 tgaaccaatt ctggaacaat cgccttcccg gcgatgatgt tggcgatgcc aatatacttg
    10501 atcttgacca gtaagcgagc aagccagtaa gtgacccagg aaacccgata gagcacgatc
    10561 atcggttttt caagcagcgc tgtctccaac gtggcggttc ctgaagccgc ccagacaaga
    10621 tccgcggcat ttaccgcgtt gtaacgaccg ctgtcgacga tcggtatggc gaacccggcg
    10681 cgatccagct ctttctgcaa caccgctcga tccagggtac tggctcgggc acaaaagaac
    10741 tgcgcctgtc gaacccgctt caactcggca gccgcagcaa ccatcaccgg caaatgatac
    10801 gcaatctctt ttcgcctgct gcccggaagc agtgcgatca ccggtttctc tttgtccaag
    10861 ccgatttcgc gcaaagtggc atcacgtcct tggctcaccc cgacgacatc gagcaaggga
    10921 tggccgacga attctactgg catctggtgc cgctcataga actcgacttc gaacgggaac
    10981 accaccgcca ttttgtcgac cacctgggca atctgtcgaa ttcgtccccg gcgccatgcc
    11041 catacctgcg gactgacata atacagcacc ggtatcccca ggcttttagc gagcctggcc
    11101 agacgcaaat tcaattccgg gaagtcaatg agaatgagaa gcctcggccg gcgtgtccgc
    11161 aacatccggc gcaagagacg atatgccgca acgatgtttt tgatgtttcc ggccaattcg
    11221 aggagcccca ttcccgtcaa ctcggccact gtaaacacgg cctcgaaatt ggtacgttgc
    11281 agctggtcgc cggcgacgcc gaaaaatctg agatgcggat cccgcttcaa caaggctcgg
    11341 accaagctgg ccccgtgcat gtcgccggag gcttcgccca ccaccagcat gatctccttg
    11401 ccacggtcca taagtgattt gtcgatgcct tgggcagatc aaagctgagc tcgaattaac
    11461 gacgctattt ccaaagcatt gagcccgtcg atgccgctaa ccaggggggc cgttcgcgcg
    11521 cgcacacaat cgagaaacga gtcgatttcg tcggcgagag cgtctccttc cttgaattcg
    11581 atattctcgc tgcgcacgtc caaccatccc gcgccgggcg ccggtttgta aataatctgg
    11641 gcacggcgtt gatcgtagtc gattgagata taagcgtcgg gttggaagaa ccgaaccttt
    11701 cgttcccgct tgatggacac ccggctggcg gtaacgttgg cgatgcaacc gttggcaaag
    11761 gtcagtcggg cgttggcaat atcaggggtt tcggtcagta caggcacgcc gacagcctcc
    11821 gcgcggatca ccggagaacg caccaggctg gcaatgacat cgatgtcgtg aatcatcaaa
    11881 tcaagcacga cgtcaacatc agtgcctctt tcgacgaagg gtgccaagcg atggcattcg
    11941 acgaacttgg gttctttgat gactccttcc agccgccgga tcgccgggtt aaagcgctcg
    12001 agatggccca cctgcaagat acaatgctga gactccgcga gctcgaccaa ctcctttgcc
    12061 tcgtccaggg tggcggcaag cggcttttca actagcacgt cgatgccggc cgttataaag
    12121 tctctggcga cttgatggtg gacccgagtt ggaacggcaa tgctgacaca ctgcactcgc
    12181 ccgaatagct cacgataatc cgtcaacccc gcggctccga cgccactgcc gatttcagcg
    12241 gcacgggcac tatcttgatc aaccaccgcg acgagatcag ccttctccga agcggcatac
    12301 ttctccgcgt gaaatttacc tagataaccg accccgatca cggcagctaa aattttctgg
    12361 gctggatgca ttctagtttt tgttcatacg gccacgccga caaccgcgat tcccccggca
    12421 tcagcctccg caagcagcgc gtctttctcc agcaataacg ttttttccgc ttcgagagct
    12481 aaagcaaccg cgccggcatg gcgaagtatt ttgattgttt ccagaccgac ggcaggaacg
    12541 tcgaagcgca agtcttggtt cggcttgctg accttgataa ccacacaacc agagcgcgcc
    12601 aagttaccgc cccgcttgat cgcctcatcg gtgccttcta ccgcctcgac ggcgatcacc
    12661 atgccgtttt ttactaccac ggtttggcca atacccagac ggccgatttc tttcgcgata
    12721 cggatgccaa cacggatatc ctcccactgg cgcttggaag gcgcatggcg agtgagaacg
    12781 ccggcagtgg tgataatttg cggcacgcac agagtcgatt ccagaatggc aattccatcc
    12841 ttctcgagtt cgttggcgat gccccgcagt aaaccatcgt cgtctcggct tttcacacgg
    12901 gccagaaagc tggcgccgcg caagtcaggc cgaaaattgc cgaacagcct cactttgcga
    12961 atgccacccg ccatgactgc ctgggtgacc cgcgcgcgct gaaatgtgcg gataattttt
    13021 cccaactgtc caacataaac ccaggtcaat tcgtcgacaa ccttctcaat ctcggcaagg
    13081 gtctcgtcgc gatgcgcgac cgccacaata gagtatccct cgcgcttggc ttcctgagcg
    13141 aaaatgtagg ggaatcggcc gttgccggct attaaaccta gtcgcttatc atgaagagag
    13201 gtcaccgttg gtgagccctt gattgaaggt atggcactcg ttatcggcag atgccgcgtt
    13261 gagattgggc gatgaacgct aacaagatat cgatttccgc cgtgggtgat acctcgcgcc
    13321 ggatcttctc cagtgcgtct ttggttttga gcttcgattg gaaaatgatc cggtaagctt
    13381 tcttcaaggc gtcaatcgca gccggagtaa acccacggcg ttttaatcct tcgaggttga
    13441 gcccgcgaag gcgcgctcga tcaccggtgg tattgcaaaa aggcggaaca tctttggaaa
    13501 ccatcgatcc cgctcccaaa atcgcgcccg aaccaatacg ggcgaactga tgaataccaa
    13561 ccaagccacc gacgatcacg tagtcttcaa ccaccacatg cccgcccagt gtggcgccgt
    13621 tggcaataat attgtggttg ccgatcagac aatcgtgagc gatatggcaa tgcatcatca
    13681 gcaaattgtg gtcacccacg cgggtgacca tcccgccgcc ggcggttcca ggattgagcg
    13741 atacgtattc tcggatggtg ttgtggttgc cgatgatcaa ttcgctggct tctcccctgt
    13801 acttaaggtc ctgcggtctg gatccgatgg tggcgaattg atagatcgtg ttgccttggc
    13861 ccagagaagt ccgtccctca atcaccacat gggacgccac tttggttgct ctgccgattc
    13921 ttaccccggc gccgatgaca ctgtaaggcc cgatctcaac gtcagaatct atttccgctt
    13981 gacaattgat caaagctgtc tgatggatcc tgctcatcat tggtcctatt tgagtctcta
    14041 gggcatgtcg atttccatgg cggaaatttc agcttcagct acgagcttac cgtccaccaa
    14101 cgcgcttcca tgcattttcc agaagctgcc gcggcgcttc aaacacgtca attccattcg
    14161 cagttggtct ccgggttcca ccggacgttt gaattttaca ttatccaggc cagtgagaac
    14221 gaacactttg ttatcgtcca ttccaccgcg ggcgttgtga gcgaagatcg cgcccacctg
    14281 ggccatcgcc tcgcagatca atactcccgg catcactggc cgattgggaa aatggccctg
    14341 aaagaagttc tcgttgaatg agacattctt gatagcgacg attttttctt ctccgtcgat
    14401 ttccagaatt ttatcgacca gaagaaacgg atatcgatgg ggaagatatt ttagaatgtc
    14461 tttgacctcg agcataacgc tacttttcct ttggttgccg attcgcaagt tgcgcgactt
    14521 gcttttccag gcgtcgcacc agtgtccata gcttaggtag ctgcggcaac agagtcatta
    14581 ccttcagcca ctcgtgatgc ggcgccacgc caatgccgcc ggacagcaat gctcccgccg
    14641 gcaccgactg aggcactccc gattgcggtc cgatgatcgc tccgtcacca atctcgatgt
    14701 gattgactac gcctacttgt ccggccaacg tcacattttg gcccaacttt gtactgccgg
    14761 cgatccccgc ttgcgccgcg atcaccgagt ttgctcccac gtttacatta tgggcaattt
    14821 gcacgagatt atcgatcttg gctcctcgtc caatgagcgt gcggccaaga gttgcgcgat
    14881 cgatggtcgt attggcgccg atctcgacat cgtcttctat ctcgaccatg ccgacctgcg
    14941 ggatcttgat cctagcctcg ccactgccgg cgtaaccgaa cccatcactg ccaacaatca
    15001 cacccgcatg aaggatcacc ctgttgccga gccggcatcc atcacgaacc accacattgg
    15061 gatggagcac gcagtcgcta ccgatcgcaa cccgatcgcc caaaaacacc ccaggaagga
    15121 gcacagtgcg gtccccgacc tgcgcgcatt gaccgacata gacattggga tagaccgaga
    15181 catcgtgacc aagctttgag ctcggatcga tatgtgcgga aggagcaatt tctcgctgat
    15241 actcagcggc cggagcaaac agctgaagaa ttttggcgaa ggccacatag ggctgcgcca
    15301 cctctaaaaa atcttgtccc gatctcgcta gttcggaacc gacaagtccg ggcccaacga
    15361 taatcgcgct ggccttgcaa cctgatatga acctggaata acgaggatta gcgagaaagg
    15421 tgatatcgcc cgggcccgct tcttcgatcg gcgaaactct aaatatcgtt tcagagccgt
    15481 cgccgataac tcggccgccg acgtactcgg ccagatcgcg aagtgttctt tgtcgagacg
    15541 gcactgctat ttgttgcggc tgttaaaaac ttcgatcact ttgtttgtga catcgatgcc
    15601 ttggtcgctg taaagaattt ggctacgttc caagatcaaa gtaaatttgt cggactttcc
    15661 gatctcattg acgatctttt ccagctcttt cagaatatct ccggttagtg ctccctcttt
    15721 ctgccgaagt tcctgttgct ggtcagtcat ggttcgttga taattaacat atcgtctctg
    15781 cagatcaccc tccaagtttc gtcgctcgtc ctccttcatg agagggccct ttttatccag
    15841 atccgctttg agccgctcga gctcgttttt ttccttaaga agctcagctt cggccttttt
    15901 cacctgcgcc tgaaatcgtt ccttggcccg cttaccggca ttcgaatccg agatcgcacg
    15961 ttgtatgtcg ataaaaccga ttttgacgcg gtcttgggct ccaactgctg gagcccataa
    16021 cagggtcaaa aacaaaacca accaatactt cacttaagcc tccagaaggt tagattgata
    16081 gacgggacta ggcaccagct agggctggct gccaagagtg aagccgagga ccgaggtctc
    16141 atcacccgat tgcttgttca gcgggaaacc aagctcgaca cgcaaaggac cgaacgggga
    16201 catccatcgc gctccgaaac cgaccgatct tctaaggtca gtgaaactga acttatccga
    16261 gtcaccgaat gaatttccca tatcgaagaa agccacgccg cgaagcccga attgctccat
    16321 gatcggaaac atcagctcgg cgctgagaac catggcccgg tcgccgccga ctacttcggt
    16381 gtcggtgcat ggcccattcg gaccctcgca attggcgggt tcccgcgggc cgaggctccg
    16441 ttccgcaaac ccgcgcacgg aattgatgcc accgaggaaa taacgttcaa acagcggcaa
    16501 gtccttttta ccattgtcgc gttcggctaa acctacgcca tacccgaggg aacctcccaa
    16561 cgccaacaca taggcgccgc cccagttagg gtctttcaaa aggggataat gccagcgacc
    16621 gctcaaatct gatttgataa atcgattgtc gccgcccaac cccgcaaact tgaccgagaa
    16681 agccgacttg gttccctggg tcggactaaa aaaatgatcc cggctgtcgt agctgatgct
    16741 cgggctcatc gcgctcgtga gggacgtgcc cttttcatcc tgaatgctgc ccggagctgt
    16801 ggactcaacg tcgttgatgg tttcacgagt taactcgtag cccacgccgc cgcgcatata
    16861 atcccaaagc gagggtggcg gattgctagc caattgatcc gatccgagcg aaggctgttt
    16921 tctaagccgg ccgtaaaagg gcacgctgac atccttcagc ggatagctgg tattgattcc
    16981 gaaacccaac tttctctcat cgaaatcgtg gaattcccgt tctgtgttga aggcatcgat
    17041 gcccagagac atctggctgt cgttgaagta cgggtcagta aaccccacta taaagtcttg
    17101 gcggctggag ccgattgcga aactgccgtt cagtccttgg ccgcgaccaa ataaattctt
    17161 ttcggcgaca ccggcattga agatgaagcc gtcgccgctg ctaaatcccg cccccaggtt
    17221 aaatgtgccg gtgggccctt ctttaacgtc gaccaggaga tccacggtat cgggttggtc
    17281 cgtctttttt gtggtcagct gaacgtcctc aaaatatccg gtccgttgaa gcgcgtttcg
    17341 gctctgggta attttggagc tggaataaag ctcttgttcc gaagccagca attctctgcg
    17401 caccaccttg tcccgggttt tggtgttgcc tgcaacgaga actcggttga agtaaaccgg
    17461 aggccccttg gcgatgacca gcgcaacgtc tacctttttt tcatttgcgt tgactttggt
    17521 caccgggtca atttgcgcga aagcgaaccc tttattggag tagagatcgg ttaaagtcga
    17581 gatatcatca cgcagccggc tgcccctaaa gatctgcccc tccgtcaatt ttatctgctt
    17641 caacatctct tggccgcctt ggatgagatc gccgccaatc tccaccttgc caacacggta
    17701 ttgcggccct tcgtcaactc taatgaccaa ctccagtccg tcgcggccac gtaacaccac
    17761 cggctcgtcg attttgtgat cgatgtagcc gtgatcgatg tagtgattcc ctagaatcgc
    17821 aacatcattg gttaggatgt ctctgtccag gacgccccgg ttggttataa aagaaaagag
    17881 ccactcttcc ttcgtggcca tcaagccttt gagttcgctt tcgctaaacg tgcgattacc
    17941 ttcaaagctg atcttcttaa ttaaaagccg atttccctcg actatgtcga gaaccaaaac
    18001 ggcttggttg ttagattcga ccgatacggc ataatccacc tgagcgttcc cgtatccctg
    18061 ctcaccatag agctttctga ccttctcgat accttcggcc actttgctcc ggtcgagaac
    18121 cgttcgaggc gttacgccca gagcggcttc gattttatca cgcccgacct gtgaggctcc
    18181 ttgtattctt acctcgcgca cgtacggttt ctctttgact gaataaatca agacgttttc
    18241 aggcgataac tgagcttcga cgtcgtcaaa aaatcccatc ctaaaaatcc ctttgacatc
    18301 ttgttcaacg agagcgggat cgaactcggc gccggctctg cttttgatat gaagtcgaat
    18361 accgtcttcc tcgactctca cattgccggt gatcctgacg tctttgaggg taattttttc
    18421 ctgtgccctt aaggccgaag cccatgtagc tgaaagaagc aaaaaacaga aaagccctga
    18481 ccgaataatt tcagtaatag tgacttgcga tttcattctg gccaggggca agacctcttt
    18541 ggagggatgg gactgccgaa tttctaacaa attcgcctcg ttttgtaaag ttaaatcagc
    18601 ggcagtgagt gaccgggaac agctggccat cgcgcaactc caactgccgg ccgatctttt
    18661 cggcgaactc acggttgtgc gttgctatga ttagcgcgac tccccgctgt tcgttgagtt
    18721 gaaatagcaa atcatgcacc tcctgcccga tgcgaaaatc caacgaccca gtgggctcgt
    18781 cggccagaat cagttttggc tgcagaatca ccgagcgcgc caccgatact ctctgctgct
    18841 cgccgccgga taatttaccc gggcgatggc tcattcgctc cttgagtccg acggattcaa
    18901 ggagatcctc cgcctgttct cgggcctgcg ccggcgacat cccttggatc agcgccggga
    18961 gcatgacatt ctcgagggct gaaaaatccg gcaatagata atgaaactga aagacaaagc
    19021 cgatttctcg attgcggaaa gcagatagcg ccccttcgtc attcagcggc aactccttgc
    19081 ccgagtatat gattcgtccg ccagttgggc gatccaaggt cccgagtata tgtaagaggg
    19141 tgctcttgcc gacaccggat tcgccgacaa tggccaagcg ctcggcggtt tcaagttcca
    19201 agctcaattg gcgcaggaca acaatttccc tacctccttc gctgtatgat ttatcgattt
    19261 cgcgcgctga gaggagacta ctcatatcga ataatctcga cgggatccaa tttggcggct
    19321 tggcgggccg gatagatgct ggcgagcaaa caaacgatga acgatgacag cgtcacgatc
    19381 atgaaattgc tgaagtaaat acgcaccggc accgtggaga tcaagaatac atccttgggc
    19441 agctcaaacc ggtattgagc gatcaacgta cagaccagga ggccaagaag aaggcccaag
    19501 gtcgtcccga caacgccgat gatcaatccc ttgagcaaaa agatttttcg aatgctggcg
    19561 cgggtggcgc ccatcgacat caagattgca atgtccttgc gtttttccat caccaccatc
    19621 actagcgtcg agataatgtt gaaagcgccg atcaaaacca tcaacagcaa aaccaaaaaa
    19681 tagacggtct tctccaactg aagggcagaa aacaggtttg gccataaccg ggtccaatct
    19741 tcggttagat acgggaaacc caactggcgt tgaatccgat cggcgatctc acgtgattgg
    19801 ttgacgtcat caacccggat ctctatgttg ctgacggcat cgccaagctc aaaaaatttt
    19861 tgcgcatcgg caagccccat aaacaccaac gtcgagtcaa tttcactcat gccggtattc
    19921 aagagaccgg tgacgacaaa ccggcgcact tttggaatga cgccgatggc tgtgggactg
    19981 ccgagagggg agaccacctg aaccggactg ccgacaaaaa gcccaagctg cgcggctaag
    20041 cgctcgccca tgatcacgcc cgggagcatg atggtgcgat cctggacctg aaccggattc
    20101 tgcttcttga ggccggcaat gcttccttcc ttcatatagc gttgcaaatc aaccaccgca
    20161 ttaacacggt ccggatcgac gccacgaaca accacacccg aaacccgcga gccggaggtc
    20221 aacatcacct ggccgtagat ggccggcgag gcggcgacta caccctcttc cttgaccact
    20281 cgttggagta gctctttgta ttctgagagc ggatcgccgg acttcagcaa agcgatatgc
    20341 gcattgatcc cgagcaagcg atcgcgcagt gtttcttcga aaccagtcat aacggccatg
    20401 accacgttta aagtcatcac gccaatcatg acgccaagca cggaaataac cgtgatcagc
    20461 gaaataaagg tttcctgccg gcgcgctctc agatacctga ggccgataaa aagctcgtaa
    20521 ttcatcgtcg gaggatttgc tcgggtctag cgctgcggac gcagcaaagg aaataaaata
    20581 acgtcacgaa tcgatgccga attggtgagg aacatcacca gccggtcgat gccgatgcct
    20641 tcacccgcgg ccggcggcat cccatactca agcgcgcgca gataatcctc atcgatcgga
    20701 ttggcggtgt catctcctga cgcgcgcgct ttcatctgtt cgagaaagcg ctgccgctgg
    20761 tctgccggat cgttcaactc ggagaaggca ttggccagtt cgcgcccggc aataaatagc
    20821 tcaaaccgat ccaccagcgc aggatcgttg tcgttcttgc gcgccagcgg agagacttca
    20881 atgggatacc cggaaataaa tgtcggttgg attattttcg cctccgccac ctcttcgaag
    20941 acttcgacta acaatgttcc atgaggagca tcgacgtcta cctgcaatcc ccggcttttt
    21001 gcgaagcttt gtaatccgcc gagattggca atttcgtttg tctcgacgtt accataaagc
    21061 gcaatcgcat cggcaatcgt cattctgcgc cagggcggct ttagatcgat gacgtgctct
    21121 ccataaggaa cttcaagcgt tccggccacc tgattggcaa ggaaaacgaa caactcctcg
    21181 gttagatgca taagatcttc gaaggttgca taggcctgat agaattccag catggtaaat
    21241 tcaggattat ggcgcaccga aattccttca ttgcgaaaat tacggttgag ctcgaaaact
    21301 ctttcgatgc cgccaactac caggcgcttc aaaaacaatt cgggcgcaat tcgcaggaac
    21361 aattcaaggt cgagtgcgtt atgatgagtg gtaaacgggc gggcggcagc tccaccggga
    21421 atcgcctgca tcatgggagt ttcgacctcg agaaaatctc gctcctcaaa aaactttcgc
    21481 accagacgaa cgatccgaga gcgcctctcg aagacatccc ggacttcggg attgaccatt
    21541 agatcaacat aacgctgccg gtagcgggcc tcgacatcgg ctaaaccatg ccatttctcc
    21601 ggcaacggcc gaagacactt cgctagcagc cggacgcttt cagcctcaag ggtcaactcc
    21661 ttcgttttgg tgcgaaacag atggcccgag acgccgacta tgtcgccaac gtcgaatgat
    21721 tggaacaacg catagccgtc gtcaccgaga cgatctttgc gcgcgtagat ttgcagccgg
    21781 cctctgcgat cctgaagatg aaagaaagaa gccttgccga acttacggat accgatgatg
    21841 cgcccagcga gggagagatc gttggcaacg cccggaagat cttcctcacg acactcgttg
    21901 aatttggaga cgacttccac cgtcgtgtga ttgggtcgaa aatcattggg atagatcttg
    21961 tgacctgctt ccctgagctt ggcgagcttc tggcggcgca cttcgatctg ctccccggtt
    22021 tcttctacaa gttctttttc gaacgtcggc atcggcgtct gtgaaagcta ctattgacga
    22081 gggttgaatt caattaggcg ggcagcagaa aaccgctaaa tcccgagatt cttgcaaatt
    22141 gcgctgactg aatgggattt gttcaaggag tagaaatgaa gacctgaaac gccgaactgg
    22201 atcaacgcct cgcattggcg tgtcgcatat tcgatcccca attgagttgc cgcgttatcg
    22261 tcgttttcaa ctttgttcaa cgcttgctcc aactgcggcg gaattcgcgc gcgacataaa
    22321 gccgtgaagc ggcgaatttg gggcgcggaa acgatcggca gaacgccggg aatgatcggc
    22381 acttgaatgc ctagcttcct gacatcttcg acataacggt agtactgagc gttatcaaaa
    22441 aataattggg tgataatagc ctgagccccg gcgtcgattt tttctttaag gtaacccaaa
    22501 tccgaggtcc tgcttttagc ccgcggatga atctcgggaa accctgccac ggcaaccgag
    22561 aaacagccga aagaaattgc ctctttaact agttccacgg catgctgaaa accatccgga
    22621 tggggttgcc aattcggcat ccccgccggg ggatccccgc ccaaagccag gacattttcc
    22681 aaaccttttt gctgcaggcg cctgaggact ccgcgagcat catccttgga ctggccaacc
    22741 acggtcaaat ggcacatggt ctcgagaccg cattcgcgat gaattcgatc cacgagatca
    22801 acggtttttt cgcgcgtgct tccgccagcc ccgtatgtaa cagaacaaaa agcgggtctc
    22861 agcgatttga tgaattcgat ctcgccgaac agggcttcct cgcctttgtc tgtttttgga
    22921 ggaaaaaatt caactgagag tgttaacccg ggctgggcat aaaggtcagc taatttcact
    22981 aaggaatatt cctgatgaat accgtagcc
//
LOCUS       DASUVS010000246        19177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_757400, whole genome shotgun sequence.
ACCESSION   DASUVS010000246 DASUVS010000000
VERSION     DASUVS010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 19177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19177
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757400"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1105)
                     /locus_tag="VF182_13540"
     CDS             complement(104..1105)
                     /locus_tag="VF182_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX6438156.1"
                     /translation="MKAELNDKTTGQVAKSDYRAPWWLPGGHLQTLYAYFLKRETHLW
                     LRRERWETPDNDFIDLDWLDSSDDSASLLVLFHGLEGCSSSHYARSLVNHARRRRWRG
                     VISHFRGCSGEHNRLARAYHSGDSSEIDWILRRLKENNPKAKIYPIGVSLGGNVLLKW
                     LGEQGDRAFEIVEKAAAVSAPLELAAAADALDRGCRKLIYTRYFLKTLKQKSLYKIAK
                     HQLQIDPRQVRAAATFRQFDDLFTAPMHGFDSAEDYWAKSSSKRQLRNIQVPTLLLNA
                     RNDPFLPAAALPEAGEVSQSVILEYPESGGHVGFVSGRFPGDFEWLPRRIFNFFNAE"
     gene            complement(1194..4139)
                     /locus_tag="VF182_13545"
     CDS             complement(1194..4139)
                     /locus_tag="VF182_13545"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX6438157.1"
                     /translation="MTTRADTTEQAVQTLARSLYRKAAEHKPSLFEGKDLLGRMIDWS
                     LEDDSLRTALFRFVDVLPSLQSSAEIGRHLEEYFARVDHALGGLVYVAQALQAGRWVA
                     PVVRRNVVSFARRFIVEEKTDELIPILSGLRAEPAAFTLDVVGEATVSDREAVAMQNR
                     YLNLLGTLSGAAAEWSPRPQIDESSVGPMPRVSLSVKLSSLCARFDPLDPEAGAVVRQ
                     RVRTILREAARLDAAITIDMAPYAFKPLTFEIFRTLLEEEEFATTPQVALAVQAYLRD
                     SGSDLAELIGWAKQRGRRIGIRLVKGAYWDSELAWAQQKNWPVPVFLQKSETDANYER
                     LSRLLLENDDIVDAAFGSHNLRSLAHAIVAAHHLGVPPNGYEIQMLYGMAEPFRRAII
                     ENGQRVRVYLPVGELLPGMSYLIRRLMENTSNTSFLRQTYSDKKDVETLIAAPITAAR
                     SASREAQTADPGQISPFRNEPAIDFARSENRAQFYKALEATSERIKRQTKAARRGEWL
                     ESINPARLEEVVGRVKVASTEEANEAIDAATGFFPKWRATPAKTRADILLRAAEIMRR
                     KHLELASWEVLEVGKGWREADGDVIEAIDYLEYYAREMLRLAEPRITQRLAGESNVYL
                     YEPRGIAAIIAPWNFPLAILTGMTAAAVVAGNCAIMKPAEQSPMMAAHLLDILREAGL
                     PRHACQLLQGDGALGAHLVRHQSIHVIAFTGSREVGLEILKQAHTTQAGQTHVKRVVC
                     EMGGKNAVIVDNDADLDEAVVHIIESAFGYQGQKCSAASRIILLNEVHDRLLSRIIDA
                     AKSLKIGPPADPRNFIGPVIDADAKERILSYIDIGIKEAQCVLQMAAPDGGFFVGPAI
                     FSEVEPQSRLAQEEIFGPVLSIIRTRKFDDALEIANRSAFALTGGVFSRSPAHIELAR
                     KEFRVGNLYINRGITGAVVERQPFGGLKLSGIGSKAGGPDYLLQFLEPRTISENTLRH
                     GFVPPANGL"
     gene            4333..6036
                     /locus_tag="VF182_13550"
     CDS             4333..6036
                     /locus_tag="VF182_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6438158.1"
                     /translation="MNEPAQSSSSTITGAEAVVEMLQAHEVELIFGLCGDTSLPFYDA
                     LARLPHGMRHILARDERSAAYMADGYARVSKKVGVCEGPSGGGVTYIVPGLAEANESS
                     VPLLCINSDISVAARGRYTLTALDQGALMRPITKWNTVLDRAEDIPAVFRQAFRQITT
                     GRPGAAHIALPFDVQNGLVNRTEVWGETKFGSCPAERSAPEVDSIERATRLLRAAARP
                     VFICGGGVVVSGAEPELLALAERISAPVATTISGKGSIDEQHPLAAGVVGSNGGTPET
                     RSIVDQADLVIFVGCRAGSVTTERWRHPVPGQVKIVHLDVDSAVPGTNYPTDAAVVGD
                     AKLALRMLDEALGDLRRPLDAALVQNAKEQKFAKFQALAESAEKPIKPERVVAALAGA
                     LDPDAIVVADAGTPCPYLSAYYELRGSGRRFFSNRAHGALGYSLSAAIGAHLAKTAVK
                     CVSVMGDGSFGFSCGELETAARYYLPITFVVIANASYGWIKAGQKSGYGKRYFSVDFA
                     PTDHAAVASAFGVKSWRVTDPVNVAMTLKEALAYGGPSLVDIVCQPLEEAKAPVSEWI
                     A"
     gene            complement(6091..6552)
                     /locus_tag="VF182_13555"
     CDS             complement(6091..6552)
                     /locus_tag="VF182_13555"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX6438159.1"
                     /translation="MISLEQANRIIAAIFARAREQSCRPMSVIVVEPGAKIKAFQKED
                     DSAMMRFEMAFGKAYAALALGRSSSLVRMRTEERPLFMQYLIRASDDQIFPEGGGMLI
                     RDPDGELLGAVGVTGDTQERDEELAAHGIRAAGLKTDEDCAQFGSKVRLKN"
     gene            complement(6660..7514)
                     /locus_tag="VF182_13560"
     CDS             complement(6660..7514)
                     /locus_tag="VF182_13560"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone YbbN"
                     /protein_id="HEX6438160.1"
                     /translation="MNDWTIEAGEENFEADVLERSHEVPVLIDFWAPWCGPCRTLGPV
                     LEKLADEYSGKFVLAKINVDESPSLAGAFGVQGIPAVKLIKDGEIAGEFTGALPEPAV
                     REMLSRYLPSEYDEQADEAAELEEQGKSAEAQAIYQSILDAEPNHPKSLLGLGRVLMN
                     AGDRDGALQTLERISPVAEERKVADRLIARLQLQGDQPVDEAILREKLAAQPDSLEAR
                     FELAQALAASDKFEEALGEFLKIVKSDREFRDDGARKAMVQIFDVLPPGDPLVDKYRS
                     ELAKVLFR"
     gene            complement(7547..7792)
                     /locus_tag="VF182_13565"
     CDS             complement(7547..7792)
                     /locus_tag="VF182_13565"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6438161.1"
                     /translation="MPKDAEFFNLKYREGSLDAKTAQLILMAVNFAIGHEHGAKLHLD
                     KARENGATEDEIQETIVYCMRPVAAKVRNFAKEIIAK"
     gene            complement(7810..8310)
                     /locus_tag="VF182_13570"
     CDS             complement(7810..8310)
                     /locus_tag="VF182_13570"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255440914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8125..8127),aa:Sec)
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX6438162.1"
                     /translation="MTDDMLIKREIVLEADAETIGRMRKEGHARGFTVYCDEAERIGG
                     DNSAPPPLAYFGLAVGFULLTQISRYGEMLKTHIDKARVLVTARFKNEGSVLRETVRA
                     EGLGVETRCEITSTEPAEKIAKVLRNAEAGCYVIQTIRNPTEVKSVYTLNGATFEPKA
                     YINKLQ"
     gene            complement(8516..9631)
                     /locus_tag="VF182_13575"
     CDS             complement(8516..9631)
                     /locus_tag="VF182_13575"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6438163.1"
                     /translation="MNRQWLGTAFFFALLLLILYCAFLILTPFLKAIAWAVILAILVY
                     PVYAWLLKLLRWQATPAALIVIVAITLLVIIPGIELGWFLTDEAITLVQTVRSMMSDD
                     GMARWAETPWVQSILIWWDVISFRLIDFKINWKEILAQGAQVSSGFIVSQVKGVAQNI
                     LLVIVNFVIALFTLFFLLRDGKDFFRRIQRLLPMDREHQERLFRNVVDAVLAVVHGSF
                     VVAVIQGLLAGLAYGIAGVPFAALWGVVTAFFALLPVGGSTLVSIPVCLYLFFQGEML
                     RGFLLLGWCLGVVGTVDNVLKPLLIGNRLGLPVLLLFFGILGGITLFGALGIVLGPVV
                     FALLRALLDLYSEEYVEEERQRLSYPASFAERQRSND"
     gene            10017..10946
                     /locus_tag="VF182_13580"
     CDS             10017..10946
                     /locus_tag="VF182_13580"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438164.1"
                     /translation="MAEAQQPKKAWRIGFISSMSPVAYAHLYSAFQQGLGDLGYVDGQ
                     NVMIKARWAEGNMERLPELAAELVGDKVDLIVSTGGTLTALATKAATTHIPVVFTAGG
                     DLIRVGLISSLARPGGNLTGLNLLTTELGIKRLELLKESFPKIRRIGILGNPTNPNHA
                     MQVNETQAAAKQLGLQVQVLEARNLNEVEAVFSKLSEKSVSALLMLPDSMLNAHRDRS
                     AELATKSRVPAIFEFKEFVEAGGLMSYGTNIVAVYRRVAVYVDKIVKGAKPSDLPVEQ
                     PTNFEFVINLKTANQMGVTIPPNVLSRADKVIR"
     gene            11447..12436
                     /locus_tag="VF182_13585"
     CDS             11447..12436
                     /locus_tag="VF182_13585"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438165.1"
                     /translation="MRKISLIVLSAALLMLTVPARAQQAAKVSRIGFMAGVSPPPTEP
                     SRSNMEALRHGLRELGYVEGKNIVIESRWANGKLDRLPELAAELVGLQVDVIVTGGTP
                     AVIAAKQTTSTTPIVAASADNLVEAGVVASLARPGGNVTGSTRVDADFSAKRLELLKE
                     TFPKLSRLAVLSHGALGGDQEELEETQAVANASGVQIESFHVQDPSRFGSVYAEMKKK
                     RVDALIIFTSSFTSFHRRTLLEHATANRLPTMCAQAAWIEAGCFMSYGPNLTEFYRHA
                     AYFVDRILKGAKPADLPVERPRKYELIINLKTTNQLGLTVPPNILARADRVIR"
     gene            12531..13472
                     /locus_tag="VF182_13590"
     CDS             12531..13472
                     /locus_tag="VF182_13590"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438166.1"
                     /translation="MNVSITDAQQSQKPFRLGYIATVAADAGNPNFQAFRRGLRDLGY
                     VEGKNILIEHRSVEGRTERIPNLVNDMVQLKVDIIVSAMSAAIQGAKETTKTIPIVMI
                     AARDPVKSRFVASLAQPGGNITGVTRVTEELAGKRLELLKETVPQVTKIGVLQQVHAT
                     TSLDAMNDYEAAAHGLNLTVHSLPVRDPDLDFTPAFRDGAKERVSALITVTTPLFVRR
                     AKRITELAIKHRLPSMFEGSEYPDAGGLMSYSGNDAETFRRAAAYVDKILKGAKPADL
                     PVEQPTKFELVINLKTAKQIGITIPPSVLARADKVIR"
     gene            13563..14552
                     /locus_tag="VF182_13595"
     CDS             13563..14552
                     /locus_tag="VF182_13595"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438167.1"
                     /translation="MIRGKKILKFLPYVLLLALFSYADAQQSTNSSRRIGYLGGGSVE
                     LEKVWLDAFLQGLQELGYFEGKNIIVERRYADGRYEQLPALAAELIRLKIDIIVAAST
                     PVAQSAKKTTSSIPIVMVVADPIGTGLVASLARPGGNITGLSDFHADLVTKRLELLKE
                     VVTSLSRVAVLSNPASPTCSTQLKDLNTAGPALGLTLIAIEATKPEDLPNSFSRIKEA
                     RVGAVFVCGDRMFNTHRQQIFELVNNNQLPAIYSTKEYADSGGLMSYGANFPELYRRA
                     ATYVDKILKGAKPAELPVEQPTKFELVINLRTAKQISLTIPPNVLARADRVIK"
     gene            14549..15532
                     /locus_tag="VF182_13600"
     CDS             14549..15532
                     /locus_tag="VF182_13600"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438168.1"
                     /translation="MIKYRATVLWLIGLIFGSIHPAEAQQLAKKVTRIGVLSPGSLPL
                     GPLEAFRQTLREVGYIDGQNITIEWRFAEGKNDRLPELAEDLVRLKVDVIFVINTQAA
                     QAAKKTAGTIPIVFARVSDPTRTGLVASLSRPGGNITGLSNVADELGGKRLELLKETL
                     PHVSQLAVLWNSGNPGVALIVKEMEFASPRLNVQLHSVGMREPKDFLTASETLTKGRF
                     GALFVLDDLLVTSYKREIMDLSQRNRLPVISLYAEFVNIGGLMAYGPSIPEMYRRAAL
                     YVDKILKGVKPGELPVEQPTKFELVINLKTAKQIGLKIPPNVLARADKVIK"
     gene            15762..16757
                     /locus_tag="VF182_13605"
     CDS             15762..16757
                     /locus_tag="VF182_13605"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438169.1"
                     /translation="MTLKFFCAALTLMFGGFSDYAEGQQLGKIQRVGFLQRRIAPTAD
                     NPDPLGDALRQGLRELGYIEGKNIVIEQRYAGGKIDIVPAVIAELIQLKVDVLVVASS
                     PIIHAAKQATKTIPIVMIAQVDPVAAGWIDTLARPGGNLTGLTRMTRDLNAKRLEVLM
                     EAVPGISRVGVLVDASSPETASANLKEYEVAARGRQISIQIVEVRGPKPDFERAFQSA
                     KGRIDALITTRDALTASYPNRIAELAIQSRLPSMHADDSYVRAGGLMSYATSDSEQFR
                     RAAIYIDKILKGANPADLPVEQPTKFEFVINLKTAQLIGLAIPPNVLARADKVIK"
     gene            16754..17740
                     /locus_tag="VF182_13610"
     CDS             16754..17740
                     /locus_tag="VF182_13610"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438170.1"
                     /translation="MTVVRHQWSVVSFLLCATLCASFSHAEGQITNKLARIGYLTGSG
                     SSTSDAFRGELRGLGYADGKNITIDFHTAQGRLGNLPKLADKIAASSVDVVFAADFNS
                     AVAAKNATQRIPIVFQTLGDPVASGLVESLFRPGRNLTGVAGFGPELGGKRLEIIKEV
                     VSGISRVALLTDPSNAASAVTLRETQLAANSLNVNLQVFKVNNPIELKNAFVLMRKSQ
                     AGALLVNHDPTLTAQRTYIVRLVEEARLPAIYIESLWEQSGGLIVYGPNLSKQFRRAA
                     TYVDKILKGAKPAELPVEQPTKFELVINLKTAKQIGLTIPSNVLARADRVIK"
     gene            17878..18825
                     /locus_tag="VF182_13615"
     CDS             17878..18825
                     /locus_tag="VF182_13615"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438171.1"
                     /translation="MCGTRAEAQQAGKVFRIGFLERSTAAGSAVLLTAFREEMSKLSW
                     IEGKNITIEVRFADQKLERLPELAAELVGLKVDVIVTGGSGPTSFAKKATATIPIVFA
                     QDPDPVGNGFVRSLARPEGNVTGTSGLGTELNSKRLEILKDTVPKLARVGILRQQAGG
                     ITEGITMKEVKPAAQALKLKLEEIEIQLVDAKGLASAFQTAKQKQVNAIMWSGGRAIF
                     GERKRIVELAGEHRLPAIYFQKEFVDEGGLMSYGVDYDDLFRKSAHYVDKILKGAKPA
                     DLPVQQATKFEFVTNLKAAKQIGLTIPVNVLERANRVIK"
     gene            18830..>19177
                     /locus_tag="VF182_13620"
     CDS             18830..>19177
                     /locus_tag="VF182_13620"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX6438172.1"
                     /translation="MDFPILDFGFKAGYLDATTASASAAQLETFRQELSKLGWIDGKN
                     LVFEYRFAENKPQRVPELAAELVRLKVDLIVTSGNPPALAAQKATSTIPIVMGATNPV
                     ALGLVASLARPGGN"
BASE COUNT         4482 a         5352 c         5249 g         4094 t
ORIGIN      
        1 gtcatctccg gcgggccgaa gccgggaaca agcttctctt cgctcaatga agtttttacc
       61 ccgtgaatca cgttagacaa gcgccggagt ggggcagcca ctgttattcg gcattgaaga
      121 aattaaaaat ccgtcgcggt agccattcga aatcgcccgg aaatcttccg gatacgaatc
      181 caacatgacc gccggattcc ggatattcca gaatgaccga ttgggaaact tctccggcct
      241 ccggcaatgc agcagccgga agaaacgggt cgttgcgcgc gttgagcagc agagtcggca
      301 cctggatatt tctcagctgc cgcttgctgc tcgacttagc ccagtagtct tcggcgctat
      361 cgaagccgtg catcggagcg gtaaagagat cgtcgaactg ccgaaatgtc gctgccgcgc
      421 gcacctggcg cggatcgatt tgtaactggt gcttggcaat cttgtacagt gacttctgct
      481 tgagcgtttt gaggaaatag cgcgtgtaga tcagcttgcg gcaaccccga tccaacgcat
      541 cggcagcagc cgctagctcc agcggagctg agaccgccgc cgccttctcg acaatctcaa
      601 acgcccggtc accttgctcg cccaaccatt tgagcagcac gttgccgccg agcgagacac
      661 cgatgggata aattttcgct ttcggattgt tctctttcag ccgccggaga atccagtcaa
      721 tctcactcga atcgcctgag tgatacgcgc gcgccaggcg gttgtgctcg ccactgcagc
      781 cgcgaaaatg cgaaatcacg ccgcgccaac gccggcggcg cgcatggttc accagactcc
      841 tcgcatagtg actgctcgaa cagccctcca gcccatgaaa aagcaccagc agactcgcac
      901 tgtcgtcgct ggagtcgagc caatccagat cgataaaatc gttatcggga gtttcccagc
      961 gttcgcgccg caaccaaaga tgcgtctcgc gcttgagaaa gtaagcatag agggtctgca
     1021 gatgaccgcc cggcaaccac cacggcgcgc gataatcaga cttggcgact tgaccggttg
     1081 ttttgtcgtt aagctcggct ttcattcgtc gactgagtct ggccggatgc gcacacattg
     1141 ttggcaggtc tgacgacttg aaccatttga gcgatttaga cggcttgaac gattcagagt
     1201 ccgtttgctg gcggcacaaa accatggcgc agggtgttct cggagatggt ccttggctca
     1261 agaaactgca gcagataatc cggcccgccc gctttcgacc cgatgccgga cagtttgagc
     1321 ccgccgaacg gctggcgctc aaccacggca ccggtgatgc cgcgattgat gtacaagttg
     1381 ccgacccgga attccttgcg cgccagttca atatgcgccg gcgagcggga aaatactccg
     1441 cccgtgaggg cgaatgcgga gcggttggca atttcgagtg cgtcatcgaa cttccgtgtt
     1501 ctgatgattg agagcaccgg accaaaaatc tcctcctgag ctaaccggct ctgcggctcg
     1561 acttcgctaa agatcgccgg gccgacaaag aagccgccat ccggcgcggc catctggagc
     1621 acgcattgcg cctccttgat accgatgtcg atgtaactga gaattcgttc cttggcgtca
     1681 gcatcgatca ccggtccgat gaaattgcgc ggatctgccg gcgggccgat cttgaggctt
     1741 ttcgccgcat cgatgatccg cgacagcaac cgatcatgca cttcattgag cagaatgatc
     1801 cgcgaagcgg ccgagcactt ctggccctgg tagccgaacg ccgattcgat gatatgaaca
     1861 acggcctcgt cgagatcggc gtcgttatca acgatcaccg cgttcttgcc acccatctcg
     1921 cataccacgc gcttgacatg agtttggccg gcttgagtgg tatgggcctg cttcaatatt
     1981 tccagcccga cttcgcgcga gccggtaaac gcaatcacgt gaatactctg atggcgcacg
     2041 agatgcgcgc ccaaggcacc gtcgccctga agcaattgac acgcgtgccg tggcaatccc
     2101 gcttcacgta agatatcgag cagatgagcg gccatcatcg gtgactgctc ggcgggcttc
     2161 atgatcgcac agttcccggc gacgaccgcg gcggcggtca tgccggtgag aatggccaat
     2221 ggaaaattcc atggcgcaat gatcgcggcg atacctctgg gttcgtaaag atagacattg
     2281 ctctcgccag cgaggcgttg cgtgatccga ggttcggcca gccggagcat ctcgcgggca
     2341 taatattcga gataatcgat cgcttcgatg acatcgccgt ccgcttcacg ccagcccttg
     2401 ccgacttcaa gaacttccca ggacgccagc tccagatgtt tgcggcgcat aatttccgcc
     2461 gctcgcagga gaatgtctgc tcgcgttttg gcaggcgtgg cgcgccactt cgggaaaaaa
     2521 cccgttgccg catcgatagc ctcgttggcc tcttcggtgc tggctacctt cacgcggcca
     2581 acgacctctt caagcctggc cggatttatc gactccaacc actcaccgcg ccgtgccgcc
     2641 ttcgtctgtc gtttaatccg ctcgctcgtg gcctcgagcg ccttgtaaaa ttgcgcccgg
     2701 ttctccgacc gcgcaaaatc gattgccggc tcattgcgaa agggcgaaat ttggccggga
     2761 tcggcggttt gcgcttccct cgaagcgctc cgagcggccg tgatgggtgc ggcgatcaaa
     2821 gtctctacat ccttcttgtc agaatacgtc tgccgcagga aagatgtatt cgaagtgttc
     2881 tccatcagac ggcggatcag ataggacatt cccggcagca gctcgccgac cggaaggtag
     2941 acacgaaccc tctggccatt ttcgatgatc gcccggcgaa acggttcggc catgccgtag
     3001 agcatttgaa tttcatagcc attaggcggc acgcccaaat gatgggctgc gacaatcgca
     3061 tgggcaagac tacgcagatt gtggctgccg aaggcggcat cgacgatgtc atcgttttcc
     3121 agcaacaagc ggctaagccg ttcataattg gcatcagttt ccgacttctg caaaaatacc
     3181 ggaacaggcc aatttttctg ctgcgcccat gccaactcgc tgtcccagta ggctccttta
     3241 accagccgaa tgccgatgcg ccggccgcgc tgtttggccc agccaatcag ctcggccaaa
     3301 tcgctgccgg agtcacgcaa ataagcttgc acggccaagg caacttgggg cgttgtcgca
     3361 aactcttcct cttccaagag cgtgcgaaaa atttcgaatg tgagcggctt aaacgcgtac
     3421 ggtgccatgt cgatggtgat ggcggcatcc agccgcgctg cttcgcgcaa gatcgtacgc
     3481 acccgctggc gcaccaccgc tcccgcctcg ggatcgagcg gatcgaagcg cgcgcacaac
     3541 gaagacagct tgaccgaaag actgacccgg ggcatcggtc caaccgacga ctcatcaatt
     3601 tgcggccgcg gagaccattc cgccgctgct ccagacaaag tgccgagcag attgaggtag
     3661 cggttctgca tcgccacagc ttcgcgatca ctgactgtgg cttcgccgac cacgtccagg
     3721 gtaaaggctg caggctcggc tctaagcccg gagagaatcg gaatcagttc gtcagttttt
     3781 tcttcgacga tgaagcgtcg ggcgaaactc acaacattgc ggcgcaccac cggcgcgacc
     3841 catctgcccg cctgaagcgc ctgggcgaca taaaccagcc cgcctaacgc atgatccact
     3901 cgcgcaaagt attcttccaa gtgccggccg atttcggccg aagattggag gctcggcaat
     3961 acgtcgacaa agcggaacaa agcagtgcgc agcgaatcgt cttccagcga ccagtcgatc
     4021 attcgaccga gtagatcttt gccctcgaac agcgacggct tgtgctcggc ggccttgcga
     4081 tagagagacc gcgccagtgt ctgaactgcc tgctcggtgg tgtccgctcg cgtcgtcatc
     4141 tcatttgtaa atgatagctg aatcgctagc gatccgttga tcgccactgt ttgtgtttct
     4201 ctagaccatc ataatacgat cggcgccagc ggtgaaagac agctcgccga tcgcgcgttt
     4261 ttttctgcgc aacgaagtga gcaacgtatg gcgtgagagc acggtagaat agatcaaaga
     4321 atgaaacatt ctatgaacga gcctgcccaa tcatcgtctt caaccatcac cggcgccgaa
     4381 gcggtggttg agatgctgca agcccacgaa gtcgaattga tcttcggcct atgcggcgat
     4441 accagtttgc cgttttacga cgcgctcgcg cgcctgccgc atggcatgcg tcacatcctc
     4501 gcacgtgacg agcgctccgc cgcgtacatg gcggacggct atgcgcgagt atcgaagaaa
     4561 gtcggggtgt gcgagggccc gagcggcggc ggcgtgacgt acatcgttcc cgggttggct
     4621 gaagccaatg aatcttccgt gccgctgctc tgcatcaatt cggatatttc cgtcgccgcg
     4681 cgcggccgct ataccttgac ggcattggat caaggcgcgc tgatgcggcc gataacgaag
     4741 tggaacactg ttctcgatcg cgccgaagat ataccggcgg ttttccgcca ggcgtttcgg
     4801 caaatcacca ccggccgtcc aggtgcggcg catatcgctc tccccttcga cgtacaaaat
     4861 ggtttagtga atcgcactga agtttggggc gagacaaagt tcggcagctg tccggccgaa
     4921 cgcagtgcgc ccgaagtcga ttcgatcgaa cgcgctactc ggctgttgcg cgcggcggcc
     4981 cggccggttt ttatttgcgg cggcggtgtc gtagtctccg gagcggagcc ggaactgctc
     5041 gcccttgccg agcggatctc ggctccggta gcgacgacca tcagcggcaa aggctccatc
     5101 gacgagcagc atccgcttgc ggccggcgta gtcggctcca acggcgggac acccgaaact
     5161 cggtccatcg tagatcaagc ggacctggtc attttcgttg gctgccgcgc tggctcggtg
     5221 acgactgaac gctggcgcca tcccgttcca ggccaggtga aaatcgttca tctcgacgtc
     5281 gactccgcag tgcccggaac gaattatcca accgacgccg ccgttgttgg cgacgccaaa
     5341 ctggcactga gaatgttgga tgaagcgctc ggagacctgc ggcggccact cgatgctgca
     5401 ctcgtgcaaa acgcgaaaga acagaaattc gccaagttcc aggcgctcgc ggaatcggcg
     5461 gaaaaaccaa tcaagcctga gcgcgtcgtg gccgcgcttg ccggcgcgct cgatcccgat
     5521 gccatcgtcg tcgccgacgc cggcacgcca tgtccctact tgtctgctta ttacgagttg
     5581 cgcgggagcg gtcggcggtt cttttcgaat cgtgctcacg gggctctcgg ttactcgctg
     5641 tccgccgcga tcggcgctca cctcgccaag actgcagtaa aatgcgtctc ggtgatgggc
     5701 gatggcagct ttggatttag ctgcggcgag ctcgagaccg ccgcgcggta ttacctgccg
     5761 attacttttg tggtgattgc aaacgccagc tacggctgga tcaaagccgg tcagaagtcc
     5821 ggctacggca aacgttactt ctcggtggat ttcgctccca ccgatcacgc cgccgtcgcc
     5881 agcgccttcg gcgtcaaaag ctggcgtgtg accgaccccg taaacgttgc gatgacgctg
     5941 aaagaagcac tggcgtacgg cggtcccagt ttagtcgata tcgtctgcca gccgctcgag
     6001 gaagccaaag ctccggtctc ggagtggatt gcatgagaag gcgctaggca ttagacaaga
     6061 gttttctttc tcccctattt gcctaatgcc tcagttcttc agtcgcacct tcgagccgaa
     6121 ttgggcgcag tcttcgtcgg ttttgagtcc agcggcgcgg atgccgtggg cggcgagttc
     6181 ctcgtcgcgc tcctgagtat cgccggtgac gccgacggcg ccgagcagtt cgccatcggg
     6241 gtcgcgaatc agcatgccgc cgccctcggg aaaaatctgg tcgtcggaag cgcggattag
     6301 atactgcatg aacagcggcc gttcttcggt gcgcatgcgc accaatgacg acgagcggcc
     6361 cagtgcgagc gcggcgtagg ccttgccgaa agccatctcg aaccgcatca tcgcggaatc
     6421 gtcctctttc tgaaatgctt tgatcttggc gccgggctcg acgacaatca cgctcatagg
     6481 ccggcagctc tgctcacgag cgcgggcaaa gatggccgcg atgatccgat tggcttgctc
     6541 cagagaaatc atcccagcct cctcgcttgc gcaagcttcg attcagtcat ctgcgatgat
     6601 tgaccacatc taactttggc cctgccaagc cgaaggtttg tagccgttcg ctaggaactt
     6661 tagcggaaca gaaccttagc aagctcagat cggtacttat cgaccagcgg gtcacccggc
     6721 ggtaaaacgt cgaatatttg caccatcgct ttacgcgcgc cgtcgtcccg gaattcgcgg
     6781 tcgcttttca caatcttcag aaactcacca agagcctctt cgaacttgtc actggcagcc
     6841 aacgcctgag caagctcgaa gcgggcctcc aagctgtccg gctgagccgc aagcttctcc
     6901 ctcaaaattg cttcatcgac aggctgatcg ccctgcagct gcaagcgcgc aatcagacga
     6961 tcggcgacct ttcgttcttc ggcgaccggc gagattcttt ccagagtctg cagggcgccg
     7021 tcgcgatcgc ctgcattcat cagcacgcga cccaagccga gcaatgattt tgggtgattc
     7081 ggttctgcat cgagaatgga ttgataaatt gcttgggcct cagctgattt tccctgctct
     7141 tccaattcgg cggcttcatc ggcttgctca tcgtactcgg aaggcaaata tctcgacagc
     7201 atttcgcgca ccgccggttc cggtaacgcg ccggtgaact cgccggcgat ctctccatcc
     7261 tttatcaact tgacggccgg aatcccctgg acgccgaagg cgccggcaag cgacgggctc
     7321 tcatcgacat tgatcttggc gagcacgaac ttgccggaat attcgtcagc caacttctcg
     7381 agcaccggac ctaatgtccg acagggaccg caccagggcg cccagaaatc aattagaacc
     7441 ggaacttcgt gcgaacgctc cagcacgtca gcctcaaagt tctcctcgcc ggcttcaatg
     7501 gtccaatcgt tcatacgtta aacgtaatcg attcgttcga gaagatctac ttggcgatga
     7561 tctctttagc aaaattgcgc accttcgccg ccacaggacg catacaatag acaattgttt
     7621 cctgtatctc atcctccgtg gcgccattct cgcgcgcctt gtccaagtga agcttggcgc
     7681 cgtgttcgtg cccaatggcg aagttcaccg ccatcaagat cagctgggcg gttttcgcat
     7741 cgagagagcc ttcccgatat tttagattga agaattccgc gtctttcggc atgacatcct
     7801 ccttcgtgat tactgcaact tatttatata agctttgggc tcgaaagtcg cgccgttgag
     7861 cgtgtacacg cttttgactt ccgtgggatt gcggatcgtc tgaatcacgt agcatcccgc
     7921 ctccgcattg cgcaaaactt tggcgatttt ttcggcgggc tccgtggagg tgatctcgca
     7981 acgggtctca acgcccaaac cctccgcgcg aactgtttcg cgtaataccg aaccttcgtt
     8041 cttaaagcgc gcggtcacca acaccctggc tttatcaatg tgcgttttga gcatctcccc
     8101 gtaccgggaa atctgggtaa gcagtcagaa tccaactgca agcccgaagt acgcgagtgg
     8161 cggaggcgcc gagttgtcgc cgccgatccg ctcggcttcg tcgcagtaga cggtaaaacc
     8221 ccgcgcatgg ccttccttgc gcatccgccc aatggtctcg gcatccgctt ccaaaacgat
     8281 ttcccttttg atcagcatgt catcagtcat acttttacct cttcgaatcg ctatggtcgc
     8341 aagttacgtg catttcaacg atcgttgcaa cttatcagaa agctagccga attgttggcg
     8401 ccaatccaca aggagcgtcg atagatagcc gaatatcgag gctgacgaga ccgaacgtat
     8461 agcttcgatc ggcggtgtcc ctgctctccc aggatttaga ctagccgatc cagagctagt
     8521 cattggatcg ttgcctctcc gcgaaagatg cagggtagga caagcgctgt ctctcctcct
     8581 cgacatattc ttccgagtaa agatccagca aagctcgtag cagagcgaat acaaccgggc
     8641 ccaaaacaat tcccagcgct ccgaacaacg taatgccgcc caatatgcca aagaaaagaa
     8701 gcaacaccgg cagacccagg cgattaccga taagcagcgg cttcaacacg ttatcgaccg
     8761 ttccaacaac ccccagacac cagcccaaga gtaaaaagcc ccgcagcatt tcgccctgaa
     8821 aaaaaagata aagacacact ggaatcgaca ccaacgtcga gccgcccacc ggcagcagcg
     8881 caaaaaatgc cgtgactact ccccacaatg cggcaaaagg aacacccgct attccataag
     8941 ccagtccagc tagtagtccc tgaatgacgg caacgacgaa cgagccatgg accacagcaa
     9001 gaaccgcgtc gacaacgttt ctgaacaagc gctcttgatg ctcgcggtcc atgggtaata
     9061 gccgctggat acggcggaag aaatctttgc cgtcacggag caagaaaaac agtgtgaaca
     9121 gtgcaatgac gaaattaacg atcaccagca atatgttctg ggcgacgcct ttaacctggg
     9181 agacgatgaa acccgaggat acctgcgcgc cttgggccag gatctctttc caattgattt
     9241 tgaaatcgat caggcggaac gagatgacat cccaccagat cagaatactc tgtacccatg
     9301 gcgtctcggc ccagcgggcc ataccgtcat cgctcatcat cgaacgcacc gtctgcacca
     9361 atgtgatcgc ctcgtcggtc agaaaccagc cgagctcgat tcccggaatg ataacgagca
     9421 gggtaattgc aacgattaca atcagagcgg ccggcgtggc ctgccagcgc aacaatttca
     9481 gcaaccaggc gtagaccgga tagaccagaa tcgccagtat tacggcccag gcaatcgctt
     9541 ttagaaaagg cgtaagaatt agaaaagcgc aatacagaat gagcagaagc agcgcgaaga
     9601 aaaacgccgt gcccaaccat tgacgattca cacaaacccc ctcggccgta aattggctag
     9661 ccaactctac gcacaaggtt cgttgcctgc aagctatgtt gtcgccagct caggcgccgg
     9721 tggcgcagca attttgctac tggtctggag caaatctact actggctatt gcctgctaca
     9781 gggtgcagca aggcatgttt cgaagcgatt cggcaactta gcctcacccc gcggcatcgg
     9841 tcggctggta caacccttgc gctgaagaaa tttcgggggc gacagcgaga gaatacatcg
     9901 accatccacc gtccatgaaa atgcaagatc ttcgatcccg caatcagaaa tctaaaatcg
     9961 cgaatctaaa atcggccgga ttgcttgtat tcattttgct cgtgggggat gcgggcatgg
    10021 ccgaggcgca gcagccaaaa aaggcctggc ggatcggttt tatatcctca atgtcccccg
    10081 tcgcctatgc gcatctgtat agcgcatttc agcaggggct cggcgatctc ggttatgtcg
    10141 atggacaaaa cgtcatgatc aaagcacgat gggcggaagg caatatggaa cggctgccgg
    10201 agttagccgc ggagttggta ggagacaaag tcgatctcat cgtttcgacc ggagggactc
    10261 tcaccgcgct ggcgacgaaa gcggcgacaa cccatatccc tgttgtcttc accgcgggag
    10321 gcgatctcat cagagtcggg ctgatatcca gcctggctcg gccaggcggc aatctcaccg
    10381 gattgaattt gctcactacc gagctcggca tcaaacgtct cgagctgctg aaagaaagct
    10441 ttccaaaaat tcgccgcatc ggcattctgg gaaatcccac caatccaaat cacgcgatgc
    10501 aagtgaacga aactcaagcc gcggccaagc agctgggctt gcaggtccag gtgctggagg
    10561 cgcgcaatct gaacgaggtt gaggcggtgt tttcaaagct gtcggaaaag agcgtctctg
    10621 cgctgctcat gctgcctgat tcgatgctca atgcgcaccg ggaccgaagc gccgagttgg
    10681 cgaccaaaag ccgcgttcct gcgatttttg aatttaagga gttcgtcgaa gccggcggtc
    10741 tgatgagcta cgggactaat atagtagccg tgtaccggcg cgtggcggtc tatgtggata
    10801 aaattgtcaa aggcgccaag ccgtccgacc tgccggtgga acaaccgacc aatttcgaat
    10861 ttgtaatcaa tctaaaaact gccaaccaga tgggtgtgac cataccgccc aacgttctgt
    10921 caagggcgga taaggtcatc agataagtca catctcggcg agtttatcgt gagcaaacgt
    10981 caaagcgcgc tagccggttt catcaccgct tcccctgctt atcaacgcac ttgggctcag
    11041 aaattaatga gacaaacagc cggcaataga ctatggtcgg gcttcgcttg cgtccgcaat
    11101 cgggaatagc caagtcgaag taacgatctg agatcggcgg tgaagttgca gtctcctgtg
    11161 actcgcgagc tgtgcgggca tggcggaagc gtagcatgcg caatttgcag gatccacttt
    11221 agtctccaac gtgtgctgca gtaccccctc tccttccctt cggcatcgat gggcatcgag
    11281 cgaagccgat cagtgtagtc gtcatgtgga acgcgatccg ccaagtaagc acaagaccag
    11341 cgaggagaga ccgcaggcag agaagccctg cgacttcaga taccgtttgc cgcgtccgtg
    11401 cgccggcggt gcatcacacg atcaggtagc tgatagaaaa actagcgtga gaaagatttc
    11461 cttgatagtt ctcagtgccg cgctcttaat gttgacggtt ccggcacggg cgcagcaggc
    11521 ggcgaaagtt tctcgcatcg gcttcatggc gggtgtgagt ccccctccca ccgaaccttc
    11581 gcgctctaac atggaagctt tgcgccacgg cttacgagaa ctcggttatg tcgaggggaa
    11641 aaacatcgtc atcgaatccc gatgggccaa cggtaaactc gatcgcctgc cggaactcgc
    11701 ggcagaactc gtcggccttc aggtcgacgt tatcgtaaca ggcggaacgc cggcggtcat
    11761 cgccgccaag caaaccacca gcacgacccc gattgtcgcg gcaagcgcag acaacctggt
    11821 agaggcgggc gtggttgcaa gtctcgcccg gcctggggga aacgttaccg gatcgacaag
    11881 agtcgatgcg gacttcagcg caaagcggct ggagcttctc aaagaaacat ttcccaagct
    11941 gtctcgtctc gccgtacttt ctcatggcgc cttaggaggc gatcaggagg agctggaaga
    12001 aactcaggca gtagcaaacg cttcaggcgt tcagatcgaa tcctttcacg tacaggaccc
    12061 tagccggttt ggtagcgttt atgccgagat gaaaaaaaag cgcgtcgatg ccctgatcat
    12121 ttttaccagt agctttactt cctttcatcg gagaacgctc ctggagcacg ccaccgcgaa
    12181 ccgtctgcca accatgtgcg ctcaggcggc atggatcgaa gccggttgtt tcatgtccta
    12241 tggaccgaac cttactgaat tttaccggca cgctgcttac ttcgtcgacc ggatcttaaa
    12301 aggggccaag ccggcagatc ttccagttga gcgtccacga aaatacgagt tgatcatcaa
    12361 tctgaaaaca acaaaccagc tggggctgac cgtaccaccg aacattctgg cgcgagcgga
    12421 cagggtgatt cgttagttcg tgagctatgc ggcgcaacaa tcacaaacgc gttaacgctg
    12481 aaagctcaag aattgaaatc attctttgcc tgctgcccgt catattcctg atgaatgttt
    12541 ccatcactga cgcgcagcaa tcccaaaaac cattccggct cggttatatc gcaacggttg
    12601 cggcggacgc cggcaatcct aactttcagg cgttccggcg agggctgcgg gatctcggtt
    12661 atgttgaggg gaaaaatatt cttattgaac atcgctccgt cgaaggtcga acggaacgaa
    12721 tcccgaatct tgtcaacgac atggtgcaac tcaaggtcga tatcattgtc tctgctatgt
    12781 cagcagcgat ccaaggagca aaggagacaa ccaaaactat tcccatcgta atgatagcag
    12841 ctcgggaccc agttaagagt cgatttgtcg ccagcttggc gcaaccgggt gggaacataa
    12901 cgggcgttac cagagtcacg gaagaactag ctggaaaaag gttggagctc cttaaggaga
    12961 cggtgccaca ggtaactaag ataggcgtgc ttcaacaggt acacgctaca acctcgctgg
    13021 atgcaatgaa cgactatgaa gccgcggcgc atggtctaaa cctcaccgtt cattccctgc
    13081 ccgtgagaga tcctgatctt gatttcacgc cggcatttcg agacggcgcc aaggagcgcg
    13141 tgagtgcgct gataacggtt acgactccgt tatttgtccg tcgtgcaaag cgaataacgg
    13201 aacttgctat aaagcatcga ttgccgtcga tgttcgaggg aagtgagtac ccggatgccg
    13261 gcggcttgat gtcctattca ggtaatgacg cggagacatt ccgcagagcg gctgcatatg
    13321 tcgataaaat tctcaaaggc gccaagcccg ccgatcttcc agtcgaacag ccgacgaaat
    13381 tcgagctggt aatcaatctg aaaacggcca agcaaatcgg gatcacgatt ccgccgagtg
    13441 tgctggccag agccgacaaa gtaatccgat gaccgaacaa tctaaaatca aatcgaaaat
    13501 caaaaaccga tggattccgc cgcacgtgct agcacgagcg gacagcgtga tgcggtagag
    13561 aaatgataag gggcaaaaaa atactcaagt ttcttcccta tgttctgctt ctcgcgcttt
    13621 tctcgtatgc cgacgctcag cagtcaacca actcatcgcg acggataggt tacctaggcg
    13681 gtggatcggt cgaactcgaa aaagtctggc tagatgcttt tttacaagga ctgcaggaat
    13741 tgggttattt cgaaggcaag aacatcatcg tcgagcgacg ctacgcggat ggacggtacg
    13801 aacaactgcc ggcccttgcg gcggaactca tccggcttaa gatagacatc atcgttgccg
    13861 caagcacgcc ggtagctcaa agcgccaaga agaccactag ctcgatcccg atcgtcatgg
    13921 tcgtcgcaga tccgatcggt acggggctcg ttgccagtct agcgcggccg ggagggaata
    13981 taaccggcct ctcggatttt cacgccgatt tagtgacgaa gcgtttggaa ttgctcaaag
    14041 aggttgtcac gtcattgtct cgcgttgctg tgctctcgaa tccggcgagc cccacgtgct
    14101 cgacacaatt gaaagacctc aacacagccg gaccggcgct aggccttacg ctcattgcca
    14161 tagaggcgac aaagccggaa gatctcccta acagtttttc tagaataaaa gaggcacggg
    14221 tcggagcggt ctttgtttgt ggcgaccgga tgttcaacac tcatcgacag caaattttcg
    14281 agctcgtgaa caacaatcaa ttgccggcaa tttattcaac taaggaatat gcggatagcg
    14341 gcggcctgat gtcttatggt gcgaactttc ctgagttgta tcgccgtgcc gcgacgtacg
    14401 tggacaaaat tctaaaaggc gccaagcccg ccgagcttcc ggtcgagcag ccaaccaaat
    14461 tcgagctggt gatcaatctg agaaccgcca agcagattag tctcaccatc ccaccgaacg
    14521 tgttggcgcg ggcggatagg gtgatcaaat gataaagtat cgcgcaactg ttctttggct
    14581 gattggttta atcttcggca gtatccatcc tgccgaagcg cagcagctgg ccaagaaagt
    14641 cacgcgtata ggcgttctgt cacctggctc ccttcctcta gggcctcttg aggcattccg
    14701 acaaactctg cgtgaggtcg ggtatattga cgggcagaac atcaccatcg agtggcgatt
    14761 tgctgagggc aaaaatgatc gacttccgga gcttgcggaa gatctggtcc gcctcaaagt
    14821 agacgtgatt tttgtaatta atacccaggc ggcgcaggct gccaagaaga ccgcgggaac
    14881 aattcccatc gtcttcgcca gagtctcgga tccgactcga acgggcctcg tcgcgagtct
    14941 ttcacggccg ggtggcaaca tcacgggatt gtcaaacgtc gctgatgagt tgggcggcaa
    15001 gcggcttgag ttattgaagg aaacccttcc acacgtctca caacttgcgg tgctttggaa
    15061 ttctggaaat cccggcgtgg cacttatcgt gaaggagatg gagtttgcca gtcctcggct
    15121 aaacgtgcag cttcacagcg tggggatgcg agagccgaag gattttctca ccgcctctga
    15181 aaccctgaca aagggacgct tcggtgcgct tttcgtttta gatgacttgt tagtgacctc
    15241 ctacaagcgc gaaatcatgg atttgtccca aagaaatcgg ctgcctgtga tttccctgta
    15301 cgctgaattc gtcaacatcg gtggtcttat ggcctatggg ccgagcatcc cggagatgta
    15361 ccggcgagcc gcgctgtacg tggacaaaat tctcaagggt gtcaagccgg gcgaattgcc
    15421 agtggaacag ccgacaaagt ttgaactcgt tatcaacctc aaaactgcca aacagatcgg
    15481 tctgaagatt ccgccgaatg tgctggcgcg ggcggacaaa gtcataaagt gaaggtcctg
    15541 aggagcaaac ggtcactcgt cagccgaagc gtgatcgagc cggctcttgc cgtcctacct
    15601 tttgcgatgt gctcccccat taaagcgcag gatgaagggc aggagccgat gaaaattctt
    15661 cgcattggat ttctcatttg cagatcggtc ttaccattcc tccgaatata aggttaaaga
    15721 ggcgataagg ttatctgctg agacgtaacg agaagacctc aatgacttta aaatttttct
    15781 gtgctgcttt aactcttatg ttcggtggtt tttccgatta tgcggagggg cagcagctag
    15841 gaaaaataca acgagtaggg tttttacaga gacgaatcgc tcctaccgcc gacaatccgg
    15901 accctctcgg tgatgcctta cggcagggat tgcgagagct cgggtatatt gaaggcaaga
    15961 atatcgtaat cgagcaacgc tatgcgggcg ggaaaattga cattgttcct gctgtaatcg
    16021 ctgagctgat acagctgaaa gttgatgtgc tggtcgttgc tagttcaccg ataattcatg
    16081 cagccaagca ggcgaccaag accattccaa ttgtcatgat tgcccaagtg gacccggttg
    16141 ccgccggatg gatcgacaca ctggcgcgac cgggaggaaa tctcaccgga ctaacccgaa
    16201 tgacccgaga cctaaatgca aaaagattgg aagtactaat ggaagcggta ccaggaatat
    16261 cacgggtggg agttcttgtc gatgcaagta gtcctgagac agcaagtgcg aacctgaagg
    16321 agtatgaggt tgcagcgcga gggcgacaaa tcagcattca aatagtagaa gtgcgcggtc
    16381 cgaagcccga ttttgaacga gcattccaat cggccaaggg gcgcattgac gcacttatca
    16441 ctactcgcga tgcgttgacg gcaagttacc ctaatcgaat tgcagagctt gcaatccaaa
    16501 gccgacttcc ttcaatgcac gcggatgatt catacgttag ggccggcggg ctcatgtcgt
    16561 atgctaccag cgactccgag cagttcagac gcgctgcaat atatattgat aaaatattga
    16621 agggtgccaa ccccgctgat ttgcctgtag aacaaccgac taagtttgaa ttcgttatta
    16681 acctgaaaac cgcacaactg atcggcctcg cgattccgcc gaatgttctg gcgcgggcgg
    16741 ataaggttat taaatgacag tcgtcaggca tcaatggtcg gtcgttagct ttttgctttg
    16801 cgccacgctc tgtgcatcgt tctcccatgc tgagggccag ataactaaca aacttgcacg
    16861 aatcggttat ctgaccggta gcggctcatc tactagtgat gcctttaggg gcgaattgcg
    16921 ggggttgggc tacgcggacg gcaaaaacat caccatcgac ttccacacag cgcagggccg
    16981 ccttggtaat ctaccaaaac ttgccgataa aatagcggct tcatctgtgg atgtcgtttt
    17041 cgccgccgat tttaattctg cggtcgcggc taagaatgcc actcaaagga taccgatcgt
    17101 ttttcagaca ctcggagatc ctgtcgcaag cggcttggtt gagagcctgt tccgacctgg
    17161 acgaaatcta actggcgttg ccgggtttgg gcccgagctg gggggaaagc ggctcgagat
    17221 cattaaagaa gttgtttccg ggatttcccg ggtggcgctt ctaacggatc cctcgaatgc
    17281 ggcgagtgct gtgactttgc gcgaaacaca gctggccgcg aattcactca acgtgaatct
    17341 gcaagtgttt aaagtaaaca acccgatcga gctaaagaac gcgttcgtat tgatgcgaaa
    17401 aagccaggca ggtgctttgc tggtcaatca cgatccgacc ctaacggcac aacgcactta
    17461 catcgttcgc ctagttgaag aggctcgcct cccggctatc tacatagaat cactatggga
    17521 acaaagcgga ggactgatag tttacggacc caatctgtcg aagcaatttc gtcgtgctgc
    17581 tacttacgtg gataaaattc tcaaaggcgc gaagcctgcg gaattgcctg tggagcagcc
    17641 gaccaagttc gagctggtaa tcaatttgaa gaccgccaaa cagatcggcc tcacaattcc
    17701 gtcaaacgtg ttggcacggg cggacagagt gatcaagtga attttggatt ttcgatttaa
    17761 cgccaagaga acaaactgat gaacgcattc ccttcgaagt gtctttccga caatcggaaa
    17821 tcggcaatcc aaaatccaaa atggttagtg attgttactc tcgctgtcgc atgcgcaatg
    17881 tgtgggactc gcgccgaggc gcaacaggca gggaaagtct tccgcatagg gttcctggaa
    17941 cgaagcacgg ctgccggtag cgcggttctc ttgactgcgt tccgggaaga gatgagcaag
    18001 ctgagctgga ttgaggggaa aaatatcacg atcgaagtcc ggtttgcaga tcaaaaactt
    18061 gagcgcctgc ctgagctggc ggccgaactg gtgggtctaa aggtagacgt gatcgttacc
    18121 ggtggctcgg gaccaacgag tttcgctaag aaagcaaccg cgacgattcc cattgtcttt
    18181 gcgcaggatc ccgatcctgt ggggaatggg ttcgtccgca gcctggcgcg gccggaaggc
    18241 aatgtcaccg gaacctcggg tttaggaacc gagctaaaca gcaaaaggct tgaaatactc
    18301 aaggatacag tgcccaagct cgcccgagtt ggaattctgc ggcagcaggc aggcggcata
    18361 acagaaggca ttacaatgaa agaagtcaag cctgcggcgc aggcgctaaa gctaaaattg
    18421 gaagagatcg agattcaact cgtcgacgcc aaaggtttag cgagcgcctt tcaaacggca
    18481 aagcagaagc aggtcaacgc gattatgtgg agtggcggtc gcgccatttt cggcgaaaga
    18541 aagcgcatcg tggagcttgc cggcgaacac cggctgcctg ctatttattt ccagaaggaa
    18601 tttgtcgacg agggtggcct catgtcctac ggagtggact acgacgatct tttccgcaag
    18661 tccgcccact acgtcgacaa gatcttgaaa ggcgccaaac cggctgatct tccggtacag
    18721 caggcgacaa agtttgagtt cgtcaccaac cttaaagcag caaaacagat tggtctgaca
    18781 attcccgtca acgtgcttga gcgggcaaat agagtgatca aataacattt tggattttcc
    18841 gattttagat tttggattta aagccggtta ccttgatgca accactgctt ccgctagcgc
    18901 ggcccagttg gagacattcc gccaggagct gagcaagctc ggctggatcg acggaaagaa
    18961 tctcgttttt gaataccggt ttgccgagaa taaacctcag cgcgtacccg agttggcggc
    19021 agaattagtt cgtctcaagg ttgatctgat cgtgacctca gggaacccgc cggcattggc
    19081 agcccagaaa gcgacctcga ctatccccat agtcatgggc gctacaaacc ccgtggctct
    19141 aggtctggtt gccagcctgg cgcgaccagg cgggaat
//
LOCUS       DASUVS010000247         4692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1850290, whole genome shotgun sequence.
ACCESSION   DASUVS010000247 DASUVS010000000
VERSION     DASUVS010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850290"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /locus_tag="VF182_13625"
     CDS             <1..1369
                     /locus_tag="VF182_13625"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002648191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6438173.1"
                     /translation="DGLGWLDFLQRFGLGGCLADDMGLGKTVQVLALLESRRDSYARG
                     ERQTPRPSLVVVPRSLVFHWKRESARFTPKLQFLDHTGSFRAKPGEHFSDYDIVITTY
                     GTLRRDALEFKAQRFDYCVLDEAQAIKNAGTLSAKAARLLRADHRLAMSGTPVENHLG
                     ELWSLFEFLNPGLFGTATVFGRAGRNPDPATRNLLARALRPFVLRRTKSQVARELPAK
                     TEQTIYCDLEGREKKQYEELRTYYRARLLKDFEQGDRSRVKFQALEALLRLRQAACHP
                     GLIDKSKVGEPSAKLDLLMGQLEQVREEGHKILVFSQFTSLLAIVRDRLDRDQVAYAY
                     LDGRTRDREARVEEFQNDPNIKLFLISLKAGGLGLNLHAAEYVYLLDPWWNPAVEAQA
                     IDRAHRIGQTRQVFAYRLIARDTVEEKVLELQKSKRDLADAIITADNSLLHNLTREDL
                     ELLLS"
     gene            1714..3429
                     /locus_tag="VF182_13630"
     CDS             1714..3429
                     /locus_tag="VF182_13630"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438174.1"
                     /translation="MTYDVLIKGGRIYDGSGLPSYLGDVAVKDGRIIEIGRIDGSAHR
                     VVNADGLAVSPGFIDFHTHLDAQLLWDPLATSSCYHGVTTVIPGNCGLSLAPCREQDR
                     EVILKSFERVEAITLPALKAGVKWGFTAFGEYLAALRGNLGINVASLVGHCALRQFVM
                     GQASIERAATAAEIHQMKEVLKASVRAGAAGFSTNQNPVHMYADGTPIQSRFATDEEI
                     IELACALGEINQGAVQISRGSLGVSVPMRESVQLFDEISSRSGRPVIWQSITHRWDKP
                     NEWRQLLDLAKESIDRGVQSYPLCNARLFNNRLTMKNAQVFDDLPTWKAILFLPLEAR
                     IEAIKDPETRKKMRYEAVEEKKSSRFSRRWDLVYLINAVKPENKHLEKKSVAEIAKIR
                     GKDVIDAFLDLSLEEGLDTEFQTSSTNGDEKAVAEIIRSPYVLVGQSDAGAHLIYDAG
                     FGYSTRLLGYWVREKKIMSLEEGVRKLTFMVASIFGLQWRGLLRRGMAADLCIFDPTT
                     IRECEPEMVQDLPGNEKRFIQKATGIEMTIVNGQVLVEKGDHTGALPGSVLGNGNGHG
                     LQAAA"
     gene            3468..4190
                     /locus_tag="VF182_13635"
     CDS             3468..4190
                     /locus_tag="VF182_13635"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438175.1"
                     /translation="MYYGARGIVGLVKPTYRPGSMESFIKLMPEGVGFIPAHAGIRAG
                     NEQELQEALTVAEKKVARLAELGADVVMIMGAPPTMVRGHGADRAIADSLTKKYKIPV
                     TVATIAQVDAFRALGMKKLVGITYFQDDMNQRFARFLRDDGFEVKAMKGIDVPFKDAG
                     KTPPEAIYNFAKKTFIEHRPVDGIYMLGAGWNNLPVIEMLERDLKTTVVSSIPAQVWA
                     TKKILHLNEPVTGYGRLLAEMP"
     gene            4215..>4692
                     /locus_tag="VF182_13640"
     CDS             4215..>4692
                     /locus_tag="VF182_13640"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438176.1"
                     /translation="MRRIRILTVIVFVVAVGLSVPASTVRAQSVREKAEAEGKLMFYA
                     TFNAVDSKMLADGFKQAYPKIDATFYRATDAAIMERILTENRAGQNLWDVVVTTSFYG
                     HNLTKRGLFAAYDSPERRFFKEGYKDPQGAWTSVYTNYAAFGYNTSTVPKGSIPQSY"
BASE COUNT         1064 a         1266 c         1354 g         1008 t
ORIGIN      
        1 cgatgggttg ggctggctcg atttcctgca gcgcttcgga ttgggtggtt gtctggccga
       61 tgacatggga ctcggcaaaa cagttcaggt attggcgttg ttggaatcgc gccgcgactc
      121 ctatgcccgt ggcgaacgcc aaacgccgcg gccttcattg gttgtggtcc cgcgttcgct
      181 ggtgtttcat tggaagagag agtccgcgcg atttacgcct aagctgcaat ttcttgatca
      241 taccggttca ttccgcgcaa agcccggcga gcatttcagc gactacgata ttgtcatcac
      301 gacctacggc acgctccggc gcgatgcctt ggaattcaaa gcccagcggt tcgactactg
      361 tgttcttgat gaagcgcagg caattaaaaa tgccggaaca ttgtcggcca aagcggcgcg
      421 cctgctccgc gccgaccacc gcctagccat gagcggcact ccggtcgaaa accatttggg
      481 tgagctctgg agtttgttcg aatttctcaa ccccggtctc ttcggcactg ccaccgtatt
      541 tggccgcgcc gggcgcaacc ccgatcccgc cacccgtaat ttgttggcgc gggcgctgcg
      601 gccgtttgtg ctcaggcgca ccaaaagcca ggtggcgcgt gagcttccgg cgaagaccga
      661 gcaaacgatt tactgtgatc tggaggggcg cgaaaagaaa cagtatgagg agttgcgcac
      721 ctactaccgt gctcggttgt tgaaagactt cgaacagggc gaccgtagtc gcgtgaagtt
      781 ccaggctctc gaggcgctgc tgcgtttgcg acaggctgct tgccatcccg gcctcattga
      841 caagagcaaa gttggcgaac ccagcgctaa gctcgatctt ttgatggggc aactcgagca
      901 agtgcgcgag gagggacata aaattctggt gttctcccag ttcaccagtt tgctcgccat
      961 tgtccgtgat cgcctcgacc gtgaccaagt tgcttatgcc tacctggacg gccggacccg
     1021 cgaccgtgag gcgcgggtgg aggagtttca gaacgatccg aacatcaagc tatttctcat
     1081 cagccttaag gcaggcgggc tgggactcaa tttgcacgcc gctgagtacg tctaccttct
     1141 tgatccctgg tggaacccag cagtagaagc tcaggccatc gaccgcgccc atcgcatcgg
     1201 ccaaacccgc caagtatttg cttaccgtct gattgcgcga gataccgtcg aagaaaaggt
     1261 gctcgagttg cagaaaagta aacgcgatct ggccgatgcc atcattaccg cggataacag
     1321 cttgctgcac aatctaaccc gggaagacct cgagctgtta ctgtcctgag agttttactc
     1381 gagggttcaa cggtttgaag ataagccaat agctcgatgc acaagggttg gtatctaaca
     1441 atgctgaatc gaacttaaac aggctgccgg gtctgcaata tgaaactatc cccgcctcag
     1501 cgctctctgt ctcggcggtg aaccaatccg atcttttcca tatgacagag acaaactaca
     1561 gagaagttta aacaattgta gtcaccgctc tagtcgcggc gatattcaat ctggaacctc
     1621 gtctccctct ccgttagcca ttcccgattg acttttgcgc actcgtctct ataagctcac
     1681 gcagtcggtt ggatggacca aggaggatct ctcatgacct atgacgtact catcaaaggc
     1741 ggccgcattt acgatggcag tggattgcct tcctatctcg gcgatgtggc ggttaaagac
     1801 ggccgcatca ttgaaatcgg ccggatcgac ggcagcgccc atcgagtcgt gaatgccgac
     1861 ggacttgcgg tgtcgccggg cttcatcgat tttcacacgc acctagatgc ccagctctta
     1921 tgggatccac tggccacttc ttcctgttac cacggtgtca cgaccgtcat tcccggaaat
     1981 tgcgggctgt ctttggcgcc ctgtagggaa caggatcgcg aggtgatact gaaaagcttc
     2041 gagcgcgttg aagcgattac gctcccggct ctgaaagccg gagtgaagtg gggctttact
     2101 gcgtttgggg agtacctcgc cgcccttcgc ggtaacctcg ggatcaacgt cgcatcgctc
     2161 gtaggccact gtgcattgcg ccaattcgtt atgggccagg cttccatcga gcgcgccgcg
     2221 accgcggcgg aaattcatca gatgaaagaa gtgctaaaag ccagcgtgcg cgcgggcgcc
     2281 gccggttttt cgaccaatca aaatccggtt cacatgtatg ccgacggcac gccgatccag
     2341 agtcgctttg ccaccgacga agaaattatt gagctcgctt gcgcgcttgg cgaaatcaac
     2401 cagggcgccg tgcagatttc ccgcggctcc ctcggtgttt ccgtgccgat gcgcgagagc
     2461 gtgcagttgt tcgacgagat ctcgagtcgt tcgggccggc cggtgatctg gcaaagcatc
     2521 acgcaccgct gggataagcc caacgaatgg cgccagcttt tggatctcgc caaagagtcg
     2581 atcgaccgcg gtgtgcaatc ctatccgtta tgcaatgcgc gtctgttcaa caaccggctc
     2641 acgatgaaga acgctcaggt attcgatgac ctgccgacct ggaaagcaat cctgttctta
     2701 ccgctcgaag cgcgcatcga agcgatcaaa gatccggaga cccgcaagaa gatgcgctat
     2761 gaggcggtag aagaaaagaa gtcctcgcga ttttcgcgcc gctgggatct ggtttatctc
     2821 atcaacgccg tcaaaccgga aaacaagcac ctggaaaaga agagcgtggc ggaaatcgcc
     2881 aaaatccgtg gcaaggatgt catcgatgct tttctcgatc tctcgctcga ggaaggattg
     2941 gacactgaat tccaaacctc cagcaccaat ggggacgaga aggccgtggc cgagatcatc
     3001 cgcagcccct atgtgctggt cggccagtcc gacgctgggg cgcatctaat ctacgatgcc
     3061 ggctttggct actcgactcg cctgctcggt tactgggtgc gcgagaagaa aatcatgagc
     3121 ctcgaagagg gtgtgcgcaa gctgacgttc atggtcgcat cgatttttgg cctgcaatgg
     3181 cgcggcttgc tgcggcgcgg gatggccgcg gatttgtgca tctttgatcc gacgacgatc
     3241 cgcgaatgcg aaccggaaat ggtgcaagac ttgccgggga acgagaaacg cttcatccag
     3301 aaggcgaccg gcattgagat gacgatcgtc aatggccaag tgttggtaga aaaaggcgat
     3361 cataccggcg cgcttcctgg tagtgttttg ggaaacggca acggccacgg gctgcaagcg
     3421 gcggcgtaga aattaaagcg gagtaagagt taaggagaga acgcccaatg tactacggtg
     3481 cgcgaggaat cgtcgggtta gtaaagccaa cctaccggcc gggatccatg gaaagcttca
     3541 tcaagctcat gccggagggg gtcggcttta ttcccgctca cgcaggcatt cgcgccggca
     3601 atgaacagga gctgcaagaa gccttgacgg ttgccgaaaa gaaagtggct cgcttggcgg
     3661 aattgggcgc ggacgtggtg atgatcatgg gggcgccgcc gacaatggtg cgcggccacg
     3721 gtgccgaccg tgcgatcgcc gattcgttga ccaaaaaata taaaattccc gtgaccgtgg
     3781 cgaccatcgc tcaggtggat gctttccggg cgctgggaat gaaaaaactg gtcggcatta
     3841 cttattttca agacgatatg aaccagcggt tcgcaaggtt tttgcgggac gacggtttcg
     3901 aagtcaaagc gatgaaaggg atcgatgttc ctttcaaaga tgccggcaaa actccgcccg
     3961 aagcgatcta taatttcgcc aaaaagacct ttatcgaaca ccggccggta gatggcattt
     4021 acatgctcgg cgctggctgg aacaatttgc cggtgatcga gatgctcgag cgagatctca
     4081 agactacggt ggtgtcgagc attcccgcgc aggtgtgggc caccaagaaa attctgcacc
     4141 tcaacgaacc ggtaaccggt tacggccggc tgctggcgga gatgccgtaa aatccatgat
     4201 ggagcgttga agtgatgaga aggattcgga tcctgacggt tattgtgttt gtggtcgctg
     4261 tcggactatc ggtgccggct tcaaccgtta gagcgcagag cgtaagagaa aaggctgaag
     4321 ctgaaggtaa gttgatgttt tatgccacct tcaacgccgt cgactcgaag atgctggccg
     4381 atgggttcaa gcaagcctat ccaaagatcg acgccacctt ctaccgagct accgacgccg
     4441 ctattatgga aaggatcttg accgaaaatc gcgccgggca gaatctctgg gatgtcgttg
     4501 tgacgacaag tttctacggt cacaacttaa ccaaacgagg tctgtttgca gcctacgatt
     4561 cacccgagcg caggtttttc aaagagggct acaaagaccc gcaaggagcg tggacttcgg
     4621 tctataccaa ctacgccgcg ttcggctaca atacgagtac ggtgcccaaa ggcagcatac
     4681 cccagtccta tg
//
LOCUS       DASUVS010000248        21992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_101853, whole genome shotgun sequence.
ACCESSION   DASUVS010000248 DASUVS010000000
VERSION     DASUVS010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 21992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21992
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101853"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..1378
                     /locus_tag="VF182_13645"
     CDS             314..1378
                     /locus_tag="VF182_13645"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6438177.1"
                     /translation="MGFTRLKILLIAVFLFTHQWIAGAWAQSKKPVSTAELAAYAGAD
                     REQRLVTGARAEGKVVWYTSLAGSSYKDLANGFEAKYPGIKVETYRGTSTDIMTRITA
                     EAQAKQILADVLETTVPPLTYLRENKLLVAYTSPHLKNFPANTKEDAGRGLVYWAVDR
                     ETYSGVGYNTRLIPASAVPKKYDDLLKPELKGKIGFVSNETGVRAIGAMVKLKGEEFV
                     KKLKAQEVILQSISGRAMADLVVSGEVPLSPTIFRDHAIEVKGKGAPVDWVPMDVVPT
                     NTGGVAITAQAARPHAAVLMADFLLSPEGAKILGDLNYGGPLKPTPYKLWYPEAGMSM
                     AQYEQSMERWEKLLREIGRK"
     gene            complement(1799..2806)
                     /locus_tag="VF182_13650"
     CDS             complement(1799..2806)
                     /locus_tag="VF182_13650"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6438178.1"
                     /translation="MLQLVLQAALTIAVLVFTLPMTVLAQITPELIEAAKKEGEVMFY
                     GAITVSSSKAVGDAFEKKYGIKLRHWRGDATELINRSLAEARAGKPGFDVTLGNEVVM
                     ASLDEKNLFAVFEPPAAKGYPKQFLDADKKMTPWRVLPYGINFNHQLLKASEAPKTWE
                     ELVDPTWKGKFGMANPGIHVTTLQFVLNLDKIIGPKWLKVVEGWAKNDPRVGRSLADT
                     IQPLTSGEVQVALGYIKDKYQYPGPIEYVRMNKYLSSLSFVAINRQAPRPNAARLFTD
                     FFVGPEAQTIFGNYGEYVFHPEADHKFKKDVKDDQIVVMRLPSKEELEDWSKKFREMF
                     R"
     gene            complement(2998..3834)
                     /locus_tag="VF182_13655"
     CDS             complement(2998..3834)
                     /locus_tag="VF182_13655"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6438179.1"
                     /translation="MSESNKSNVEVRLDLPYGGGPEGNLCVDIYLPTTAGPNPALLCL
                     HGGAWLRGSQRQYKSWGPWLAERGYGVIAVDYRLSSQVSPAWPGVWEDVCRALDWLIA
                     NAASLHIDPARIGTVGDSVGGHMAAMLSLHETTAKRIRVMVGVYGIYDLPDWWRVTQP
                     PKRTDDPVVKLMGRPYPEAKGDYEDFSPLHRLQKFAGKPATRYLIIHGDQDAIVHHNQ
                     SERFVAGLHAKDASVEDMLIPGAGHHWFTLADDNPARRRVDQEPNAAIAPVLLRFLQH
                     GV"
     gene            complement(4044..5009)
                     /locus_tag="VF182_13660"
     CDS             complement(4044..5009)
                     /locus_tag="VF182_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438180.1"
                     /translation="MATNREALHLATHSGWYRYDRSGEDWRPADRALTFWQMSCIQVD
                     PGNPERVYIGTERSGMFVSDDGGKEWLRANPNVPCLAMSSMLALPGRLLVGTTPAALY
                     VKADTGWQEVKGVRTGAVGGTFPPNPDFPARTRVLSREDKLGGRLFAGIEVGGILVSD
                     DEGASWSPANEGLTDPDLHQILPSKKYPDLVVAACGEGVSRSIDRGSRWEKVTPPGGR
                     TYGNALAEDEDGNIYLGITQDRPRTWTRAGRAKTAIFKSRDGADWELLNENMQGGVMD
                     MCAAPDGKGVLVATADGEVVEIAPSGRARTLIDGLPCITAIALGA"
     gene            5204..5611
                     /locus_tag="VF182_13665"
     CDS             5204..5611
                     /locus_tag="VF182_13665"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6438181.1"
                     /translation="MKDVNRTREYLPGSERQTSRGYSPAVITTGGRTVYLAGHGGYSD
                     ESGTRYPGDFDAQVRICFERIRAVLAKVGGTLDDIVSMTVFIVDMKNGDRFTELRRGF
                     FSEGRYPGSALIGIKELAHPEMMLEIQAIAVIQ"
     gene            5910..6905
                     /locus_tag="VF182_13670"
     CDS             5910..6905
                     /locus_tag="VF182_13670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6438182.1"
                     /translation="MEKKRTTFGLTLSNRALVTMGTKPKDLIDMAVQAENSGAIDAIW
                     VGDSILSKPRLECIPLLGAIAARTSTVKLGVACMATIAQRNPVLLALQWASLDVLSGG
                     RTWLAACMGYPASQHPMAAKELEVMGIGSKERPARLEEMIQALRVLWSDEHASFRGKH
                     YSFEDVNLLPKPAQQPCPIYIAGTPRSNQIGDRGVEVSLRRIARYADGWMTNQIELER
                     FKDYLGRLRGMLLDEGRDPNAFKTILYYGISVNADRAHAFRQAKTFLDAYYQKDFTRQ
                     GVEIWNACGPVEHCVNCVGDFIEAGVDHVTIRPIGDDLTEQLRIYLKEVLPALRS"
     gene            complement(6944..8506)
                     /locus_tag="VF182_13675"
     CDS             complement(6944..8506)
                     /locus_tag="VF182_13675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6438183.1"
                     /translation="MFEVLSNIFTSEGFEPHGHCFLWQRPLLWLYVASDSLIAISYYI
                     IPIALIIFVRKRRDLAFNWMFLMFSAFIFACGTTHLMGIWKLWEPVYWLDGSIKAITA
                     GLSVATAATLWPLIPQALALPSPRSLELVNQELQQQILERELAVQKLRETETLFRSLL
                     ESAPDAMVIVDAQGMITLINSQTEKLFGYDRQELLGKPVERLVPARFRERHQDHRGMF
                     SKNPHVRPMGTGMELFAVRRDGSEFPVEISLSPIATGREVLMSAAIRDITERKQAEGL
                     LQEKERLATLGTTAAVFAHEIGNPLNGISTSLQIVKSILETADKTDPVLQETLDIAFQ
                     EVQRLATLLSDYRTFARPQRLNLRPADLTQIIEEVLSAQLPTYRATKVVLQTQLENVP
                     GTIVDREKIKQVILNLCKNAVEAMPNGGTLTVKASAADGGIILEVTDTGEGIARGIDV
                     FQLFRTTKPEGTGLGLPIVQQIVSDHKGTITYRSEIGKGTTFTIVLPLGLSIGGEVKA
                     EPPLDSILPRGL"
     gene            complement(8575..8931)
                     /locus_tag="VF182_13680"
     CDS             complement(8575..8931)
                     /locus_tag="VF182_13680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6438184.1"
                     /translation="MSCNLLLVEDNPLSRRNLAMFLQQAEFTVHQTDNGEAALELISQ
                     VNFDVVISDFRLPGKVNGLDVLKYQSCRGSGKRLILITAFGSEQAQAEAAAVGALYFE
                     KPISLRDLLDTIQITH"
     gene            complement(9219..9545)
                     /locus_tag="VF182_13685"
     CDS             complement(9219..9545)
                     /locus_tag="VF182_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX6438185.1"
                     /translation="MPKDPLARARVRIWIDYCNTRVQRAAGYIAHNYKVEESGKELRT
                     YLENLNRQMQGREYIADDYSLADITYIPFFLRRERYQVNIGDDLPHVKAWMKRLLERP
                     AVRATP"
     gene            complement(9567..9740)
                     /locus_tag="VF182_13690"
                     /pseudo
     CDS             complement(9567..9740)
                     /locus_tag="VF182_13690"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
     gene            9906..10055
                     /locus_tag="VF182_13695"
     CDS             9906..10055
                     /locus_tag="VF182_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438186.1"
                     /translation="MVDTRLGYAISGDITIDCHIYFFTRVIARTLGDACTGVFEVTRL
                     LEEAA"
     gene            10052..10981
                     /locus_tag="VF182_13700"
     CDS             10052..10981
                     /locus_tag="VF182_13700"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438187.1"
                     /translation="MIIDTHFHAFPKKFLDLVPEQSQSDVRGVGFHGFDHQEYLDVMD
                     QHGIDIGVLSNTGGRIEKGGDRRKALELCRIINDAFAEAHAKHPRRFKAFARPPMVDL
                     DDCVRELERCYRELNMHGVILSTNLAGKYLDEPEFKPFWDALEAGAKPLFLHPANAPC
                     QANWNKYSLHQKLLWPTDSSLAISRIVYSGLFDRYPAIKLIASHLGGMILLYLDRLNW
                     REGNVECRGVPEEYFKKIYYDTAGPIRTEFIKLVYDTVGPDQILFGADYPHGRGGRDD
                     QFYPMTLDAMSKLDIPDSDKQKIYYLNAQRLGF"
     gene            11143..11499
                     /locus_tag="VF182_13705"
     CDS             11143..11499
                     /locus_tag="VF182_13705"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6438188.1"
                     /translation="MSNRLEHVAIYCKDINESIEFYRTHFGGQPTAIRKGAAGYSFCF
                     VTIDGAPSIQLMESSDKTGVHHYGFISDDIEQAAKEFKEKNARILRENRDPSGKLTTI
                     FVQDPHGLEMEVRSAR"
     gene            11615..12634
                     /locus_tag="VF182_13710"
     CDS             11615..12634
                     /locus_tag="VF182_13710"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438189.1"
                     /translation="MFGSSFKKHAFGAIILSVIWATTLVEPALGQAKHEDIAIGYASP
                     SGVMTPLYVAQEQGLFKKHGLGVKELLLLRGTGPAAAQMLVAATAPLAALGGALVEAA
                     IRGADVAYIASTSNHLIFSIYSRSEVARPEDLKGKTVAVDAKGGSIELATIIALKQFG
                     LTLGKDVSSVYLGGPVPQLGALEKGIVDATTLSAPTTLRARKMGYKELIDIGGLKLSY
                     VHTAIGVNRAFAKNNPESVETFLKAYIEAMKMTREQPDFAMKAIAKYTQITDPEALKV
                     TYETFLPAFQQRVPYVARESVQGVLNFSSSAEAKARRAEDFIDHSFLKKIEESSFVKK
                     LYGDG"
     gene            12779..14167
                     /locus_tag="VF182_13715"
     CDS             12779..14167
                     /locus_tag="VF182_13715"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6438190.1"
                     /translation="MPRDMRSWIKQLEETGELIRIAKPVHPHTQMGALLYQSREKGLL
                     FENLEGFPGWRSLGMAPANLRHAALAFDTSIEKLIPSAAERTLKRRPVELVSTGPVKE
                     VILKGDRVDLTKLPAHVAGSEETPYIASGLMVARDPDSGIRNVAFHRLQIKGPRKTGA
                     LIVPRHTREILNKHEARNQPMPVSIFIGHHPLYYMAAASTGAFEMDELELAGGLIGES
                     AKVVKCETNDLEVPFDAEIVLEGRILPNVREDEGPFSEFHDYYVAGMGKNPVIEIDCI
                     TRRHDAIFKAIQNGSEVEGCIFHKVPFGVAVYNHIKNIGGYVNLHNLLVLPGLFGIVV
                     QMTPRFYGEAKQILMGVLSSPILHPKIAIAVDEDVNIFNYWEVIWAINTRCNPQDDIV
                     VIPGTRIHPMDPTGFEPVPPGGPYWHRVGGKVIIDATKPPLCAGEQARQPFQRLKPKG
                     WETVRLEDFLPQ"
     gene            complement(14297..14677)
                     /locus_tag="VF182_13720"
     CDS             complement(14297..14677)
                     /locus_tag="VF182_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX6438191.1"
                     /translation="MKSQLNPGLSRTATYQTTLEMRARQLARDVFSTPAMIGLMERTC
                     VELTEPYLEENEQTVGIHVDVRHLAPTNIGQSVTVTAELVEKKGNKLRYAVLAINDQN
                     VKIGDGTHARAVINTARFAKDPES"
     gene            14815..15072
                     /locus_tag="VF182_13725"
     CDS             14815..15072
                     /locus_tag="VF182_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438192.1"
                     /translation="MASAEKITVILRNSSSTLTTNEFEVFDKVCNRPVGTFTLKGGES
                     RSIEISQDETGRGNLKIRNPDLGPNDWLEVSSVSRGDVVTA"
     gene            15208..15837
                     /locus_tag="VF182_13730"
     CDS             15208..15837
                     /locus_tag="VF182_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-associated protein"
                     /protein_id="HEX6438193.1"
                     /translation="MSYNLLLTDFFSCPIPFSINLLYTLFVCVLVPLYWRQYGPANFL
                     WFSDLALFIGLAALWLESSLLASMQAVSVLLLELLWNIEFFVGLIAGKQILGMAGYMF
                     KADQPLWLRGLSLFHVPLPVILLWQVCCFGYDSRAWIVQTSLFWLVMPVCFFFTRPEE
                     NINWVFGPGTPPQRKIPAGVYLLLLLIGAPLFIYLPTHVLLRAMLLKSH"
     gene            complement(16063..17313)
                     /gene="cls"
                     /locus_tag="VF182_13735"
     CDS             complement(16063..17313)
                     /gene="cls"
                     /locus_tag="VF182_13735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEX6438194.1"
                     /translation="MIRILFLIYMFLLGCAGPTQISKIDPISVGEPTFFPTIAAHTDA
                     PIMGGNKIDVLLNGEQTFPAMLKAIRGARKSITYAQYLYQDGAIAYELAEAFAERCRA
                     GLTVKLLLDSHGGSKIPEDIPQLLTDAGCQLEWFRRVRLFQFITPWELLNYNYRNHRR
                     ILVIDGILGFTGGHGVAEEWTGDGRTEGKWRDTDVRVEGPIVQQLQAAFVESWRDTTG
                     HVLGGDLYFPALKPVGKVNAQVVKSSPLGGTYESYMLLLLSITSARKSIHLSNPYFLP
                     DERMQEALLEAVKRGVSVVVLTPGKIDHKLVYWASRRGFEPLLLGGIQIYEYQAALMH
                     AKTMVVDGVWAHVGTTNLDNRSFALNEEINLIAYDRAVGGELEKAFADDLKHSKKLTY
                     EGWKARPWREKFLELFTIPLKEQL"
     gene            17904..18335
                     /locus_tag="VF182_13740"
     CDS             17904..18335
                     /locus_tag="VF182_13740"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX6438195.1"
                     /translation="MAEHDWDLFIWSRANDLLHQAERIQRNFIQIAAATQYRSSQGRA
                     PSWEPPVNVIETDESLWVISAIPGVSSDQVKVRIEANELVITGHRPLPKCCDDGDLKI
                     WEIPLGHFERRLRVIAGETRLILGEVTLKDGLLIIQLRKHS"
     gene            18332..20743
                     /gene="lon"
                     /locus_tag="VF182_13745"
     CDS             18332..20743
                     /gene="lon"
                     /locus_tag="VF182_13745"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX6438196.1"
                     /translation="MKAEPAETPKSKPRINIPEGAMVILPLRNAVLFPSTIMPLVVGR
                     PASLQAIEETVRQQLPVGFLGQRDPAVENPRPEDLYNIGTAADILRMFSLPDGQRQII
                     AQGRRRFEIAEFLQVDPLLIARVTMVEETIPQTKQFEARIVNLRQEASRALSLLPEPM
                     NELKTTIERIEDPLMLVDMIASTLDITTAEKQELLATLDPVTRAQKVSEKLARQIEVL
                     ELSKKISEETKDSMTKAQRQYFLREQLKAIQKELGEEDGKGVEAEDLRQKIYDAKMPP
                     DVEKEALNELNRLQRMPEGGPEYGSLRTYFDWLVELPWSVLSKEEIDLPKAREILDAD
                     HYDLEKVKKRIIEFLAVHKLMPEGKSPILCLVGPPGVGKTSLGQSVARAMNRKFVRQS
                     LGGVHDEADIRGHRRTYIGSLPGNIIQGIRKAGSRNPVFMLDEIDKLSASFHGDPSAA
                     LLEVLDPSQNNTFQDHYLGVPFDLSQVLFIATANVLDTIPGPLRDRMEILEVPGYIED
                     DKLAIAKGFLIPRQTVENGLKPEQIHFTDEAIREIIRSYTREAGVRQLERELGAVCRS
                     VATRVAEGLTETITIDAESIISYLGQKKFFNEIALRTSLPGVATGLAWTPVGGDILFV
                     EVTKMPGEGRLILTGQLGDVMKESAQAALSLIKSRAEALGIDPEVFKKNDLHIHLPAG
                     AIPKDGPSAGITLFVALVSLLTGRRVSKDVAMTGEISLRGLVLPVGGIREKMLAAKRA
                     GITHALLPDLNRRDIEEIPLAARGEMHFDFLKTVDDALLLALESNGAGSGFDATERPA
                     AGAVP"
     gene            21068..>21992
                     /locus_tag="VF182_13750"
     CDS             21068..>21992
                     /locus_tag="VF182_13750"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6438197.1"
                     /translation="MHKKLRLLLALGAALIVAPVYAQDPKLVEAGKKEGKVTVYGSLE
                     TDSAEAVFKVFRQKTGIDLEYWRASSTKVMDRALTEYRAGKPGFDVILTNDNPMQIMS
                     REGIFANYESPSAKEFTKESIDPKLGPRYRNVMIGILYNKSIIKPGAEPKSLEDLLKP
                     QYRGKLVMADPTQHTTTTQWVASLEKLLGKDNAEKFIRDLAATKPIMVESLLPAAERV
                     ASGETPVGITYVKYVHTFGERGAPIDYVRLGKMMGDGHYLTLGNKAPHPNAGKAFIDF
                     FLGQESMTMLAKQGEFVNRKGVYPPIAGADKI"
BASE COUNT         5036 a         6163 c         5886 g         4907 t
ORIGIN      
        1 ttttcctttc ggtcccggcc gcaccggctc cccgccctga aaatcgaagg tgatggcgac
       61 cgcaactcga ttattattgg gccacgcgat ttgccccttg gcatggttct cgacgatcat
      121 acgacctcct cggttgtcat ctcaaagatg atcgactcta aatgagtttc gttggccgtg
      181 tcaatccgtc gttggcggtg attgaaacaa aacgccgatc gcgttgacct gcatctgacg
      241 cttatgctat cgaaagtttt tagcgaaccg ccaaagattt gccgcccgtc cttcaatcgg
      301 ctacgggaga tttatgggat ttacccgact gaaaatattg ttgatcgcag tttttttgtt
      361 cacgcatcag tggatcgccg gcgcctgggc gcaaagcaaa aaaccggtct ccacggccga
      421 gctcgccgct tacgccggcg ccgatcgcga gcagcggctg gtcacgggcg ccagagctga
      481 aggcaaggtc gtttggtaca cttcgctcgc aggcagctcg tacaaagatc tcgccaacgg
      541 cttcgaagca aagtatccgg gaattaaagt cgagacctat cgaggtacga gcaccgatat
      601 catgacccgg atcacggccg aggcccaggc caaacagatc ctcgctgacg ttttggaaac
      661 gacggtgccg ccgctgactt atttgcgcga aaataaactg cttgtcgcct acacctctcc
      721 ccatctgaaa aatttccccg ccaacacaaa agaagacgcc ggtcgagggc tcgtctactg
      781 ggcggtcgat cgcgagacat acagcggcgt cggctacaac acgaggctga ttcccgcctc
      841 tgctgtccca aagaaatatg acgatctttt aaaacccgag ctcaagggga aaatcggctt
      901 cgtttccaac gaaaccggag taagagcgat cggcgcgatg gtcaagctta aaggtgaaga
      961 gttcgtcaag aaacttaagg cgcaggaggt aattctgcag tccatatccg ggcgagcgat
     1021 ggcggacctg gttgtttccg gtgaagttcc cctctccccg acaatcttcc gggaccatgc
     1081 catcgaggtg aaaggcaaag gagctccggt ggattgggta ccgatggatg tcgtaccgac
     1141 caataccggc ggcgtagcga tcacggccca ggcggcgcgg ccgcatgccg cggtgctgat
     1201 ggccgatttt cttctcagcc ctgaaggggc aaagattctc ggcgacttga actatggcgg
     1261 tcctctgaag cctacgccct acaaactttg gtaccctgaa gcggggatgt ctatggccca
     1321 gtacgaacaa tcgatggaac gatgggagaa gctgctccgg gaaatcggcc gcaaatgaag
     1381 ctcgaattca caaccttggc gccttaccaa gatccgtgat cgtcaacaaa gctcgtacat
     1441 ctactcaggc cggttgaatc ggccccccaa cggcgcctaa ggcttagctg acggtgcata
     1501 gcaggaatcc gtgttccgcg tgaaggtctc aagctattcg atcacttccg gtttcttgcc
     1561 gagaccggac atcggatcgg caacgtgcat cagatcgtca gctcaacctg gcccgcactt
     1621 ccaactgcct gcgtggaagt tccgcgattt cgcgatctgg ttctccttga gctcggacag
     1681 ctgcttcgat cgctcggcgt catcgggata gagccattga ttcggtgctc agaattctcc
     1741 cccgcgccgg tcagcggtcg ccccttagct ccaactctga gctcgagact ccattctcct
     1801 atctgaacat ttccctgaac ttcttgctcc agtcctccag ctcttctttg ctcggcagcc
     1861 gcattacgac gatttggtcg tctttcacat cttttttgaa cttgtggtcg gcctcggggt
     1921 ggaatacgta ctcgccgtaa ttcccaaaga tggtttgcgc ttcagggcca acaaaaaaat
     1981 ccgtgaacag gcgcgccgca ttcgggcgcg gcgcctgccg gttgatcgcc acgaaactca
     2041 gcgaggacag gtatttgttc atgcgaacgt attcgatcgg tcccgggtac tggtatttat
     2101 ctttgatgta gccgagcgca acctgaacct ctccggaagt cagcggctgg atcgtatccg
     2161 caaggctccg tccgacccga ggatcatttt tcgcccaacc ttcgacgacc ttgagccatt
     2221 tcgggccgat aatcttgtcg aggttgagaa cgaactgcag tgtggtcaca tggatcccag
     2281 gattggccat gccgaacttg cccttccacg tgggatcgac cagttcttcc caggtcttcg
     2341 gcgcctccga cgctttcaat agctgatggt tgaagttgat cccgtacggg agcacgcgcc
     2401 agggcgtcat ttttttgtcc gcatcgagaa actgcttggg atagcctttt gccgctggtg
     2461 gctcgaagac agcaaagaga tttttctcat cgaggctggc catgacgact tcattgccga
     2521 gggtcacgtc aaatcctggc ttgcccgcgc gcgcctcggc tagcgaacga ttgatcagct
     2581 cagtcgcatc gccgcgccag tggcgcaact tgatgccgta tttcttctcg aaggcatcgc
     2641 cgacggcttt cgatgaactg acggtaatgg cgccatagaa catcacctcg ccttccttct
     2701 tcgccgcttc gatcagctcc ggtgtgattt gcgccagaac cgtcatgggt aacgtaaaaa
     2761 caagcacggc gatcgtcagt gccgcctgca aaaccagttg caacattctc tcctcctcga
     2821 aggtttggac tcttgtatcg ccgagaatag actccggtct caggcttcgt atcggtattg
     2881 tgataactca cttaccgtta cctccacgaa cgtcattacg cagcgcggcc gcggagtttc
     2941 ctcggcgccg caacttaacg acagcgtaaa cgcatcttcg cgattcactc ggccacgtca
     3001 tactccgtgc tgaagaaacc gcaggagcac aggcgctatg gcggcatttg gctcttggtc
     3061 tacacggcgg cgcgccggat tgtcatccgc caaggtaaac cagtggtgac cggctccggg
     3121 aatcaacata tcttcgactg aagcgtcttt agcgtgcagc ccggcaacaa atcgctcgga
     3181 ttgattgtga tgcactatcg catcctggtc gccgtgtatg atgaggtacc tggtcgccgg
     3241 cttaccggcg aatttttgca atcgatgcag tggcgagaaa tcctcgtaat cccctttcgc
     3301 ctccgggtaa ggtcttccca taagcttgac gacgggatcg tcggtacgct tgggcggctg
     3361 cgttacgcgc caccaatccg gcagatcgta gatgccgtag actccgacca taacgcgaat
     3421 gcgtttcgct gtcgtttcgt gcagcgacag catcgccgcc atgtggccgc cgacggaatc
     3481 tccaaccgtc ccgatgcgcg ccggatcgat atgcagcgag gctgcgttcg caatcagcca
     3541 atccaatgcc cgacagacat cctcccacac acccggccag gctggactga cttgactcga
     3601 aaggcggtaa tccaccgcga tcacgccata gcctcgctcc gccagccagg gtccccaact
     3661 cttgtactgt cgttgcgagc cgcgcaacca cgcgccgccg tgtaaacaca gcagggccgg
     3721 attcggcccg gcagtcgtag gtaggtagat gtcgacgcaa agattgcctt ccggcccgcc
     3781 gccgtaaggc aaatccaagc gaacttcaac gttgctctta ttgctctcgc tcatatgaag
     3841 tgcttcctcg tggggaaaag cggccttgat cagatattga cctgaatatt tagctgcaca
     3901 agttcgcgtt tggttcgcgt atcatgccag cacacggcgg aaaccaatcg catcccctta
     3961 cttttgttgc cgtgaatcga aatcgtgcag gcaaaggcat tttctcccgc gcctgaataa
     4021 cgcgggctgc acccggcgac tgtttacgct cccagcgcga tcgcggtgat gcagggcaag
     4081 ccgtcgatca gcgtccgcgc gcggccggat ggcgcgatct cgacaacctc accgtcggca
     4141 gtggcgacca acactccctt gccgtcgggc gcggcgcaca tgtccatgac tcctccctgc
     4201 atgttttcat tcagcagctc ccagtcggcg ccgtcgcgcg atttgaatat cgcggtcttg
     4261 gcccgaccgg cgcgtgtcca tgtgcgcggg cggtcctggg tgattcccaa ataaatattc
     4321 ccgtcttcat cttcggccag cgcattgccg taggtacgcc cgcccggcgg cgtgaccttt
     4381 tcccaccgcg agccccgatc gatgctgcgc gatacgcctt caccgcaggc ggcaacgacg
     4441 aggtccgggt attttttcga aggcagaatc tggtgcaggt ccggatccgt cagcccttca
     4501 ttggcgggtg accagctcgc gccttcatcg tcgctgacca gaatgcctcc aacttcgata
     4561 ccggcaaaga gcctgccgcc aagcttgtcc tctcggctga gaacgcgggt acgcgccgga
     4621 aaatcgggat tcggcggaaa tgttccgcca accgcgcctg tccgcacgcc tttaacttcc
     4681 tgccaacctg tatccgcttt cacgtacagt gccgcgggcg tggtgccgac gagaagccgg
     4741 cccggcaacg caagcatcga ggacatcgcc aaacaaggca cgttggggtt ggcgcgcaac
     4801 cattctttac cgccgtcatc cgagacgaac atgcccgagc gctcagtgcc gatatacact
     4861 cgttcgggat ttcccgggtc cacctggatg cagctcatct gccagaacgt gagcgcgcga
     4921 tctgccggac gccagtcctc acccgatcgg tcatagcgat accaacctga atgagtggcc
     4981 agatgcagag cttcgcggtt tgttgccata aagcacctcc cgatggatct cacaatacgg
     5041 cgggaaagct ttttccgtca agctcattgg agcattctga acccagcgca gcgatcaaaa
     5101 cccctgattt cagtgattcg atctattctc catgtcgcgt tgcacgctag cttgctgtcg
     5161 gctatttaaa aacctccaca ttactcttat caaggagacg atcatgaaag atgtgaaccg
     5221 aacaagagag tatttaccgg gtagtgaaag acagacaagc cgaggatact cgccggcggt
     5281 tataacaacc ggagggagaa ctgtttattt ggccggacac ggcggttaca gcgatgaatc
     5341 cggcacaagg tatccgggag attttgacgc tcaggtgcga atttgtttcg agcgcatccg
     5401 cgcagttctc gccaaagtgg gcggcacgct ggacgacatt gtttcgatga ccgtatttat
     5461 cgttgacatg aaaaatggcg atcgttttac cgaactgcgc agaggctttt tctccgaggg
     5521 ccgttatccc ggaagcgctc tgatcggaat caaggagctt gcccaccccg agatgatgct
     5581 cgagatacaa gcgatcgccg tcatccagtg acgacgaccg ctggcgtggt tttaactccg
     5641 gccgtaaagc ttgtccaaag agccgctttt cttgagctcg tcgagaaagt gaagtcgacg
     5701 aattcctcgg ctcgagcttt cgcagcctta cccctgttca actgcgctcc agcaattcgt
     5761 ccggaagaat tctgaagata tttcatctgt ccttcgacga taatagagct agttctgaat
     5821 agcagtcttg gatcattggc attggcgccc gcgaacgctc gatcactccc gcttgaaaga
     5881 tgacaccgcc tcgtcctatg ttcggcggca tggagaagaa gagaactacg ttcggcttga
     5941 ccttgtccaa ccgcgcgctg gttacgatgg ggacgaaacc caaggacctg atcgacatgg
     6001 ccgtgcaggc cgagaacagc ggcgcgatcg atgctatctg ggtcggcgac agcatcctta
     6061 gcaagccccg tttggagtgc attccactcc ttggcgcgat cgccgcgcgg acttcgacgg
     6121 ttaagctcgg cgtggcctgc atggccacga tcgcgcagcg taatccggtg ctgcttgctt
     6181 tgcagtgggc gagtctcgat gttctgtcgg gcgggcgcac gtggctagcc gcctgcatgg
     6241 gatacccggc gagccagcat ccgatggcgg ccaaggaact tgaggtgatg ggcatcggca
     6301 gcaaagagcg gccggcgcga ttggaagaaa tgatccaagc cttgcgagtg ctctggtccg
     6361 acgaacatgc cagcttccga ggtaagcact attcattcga agatgtaaat cttctgccca
     6421 agccggcgca acagccttgc cctatctaca tcgccgggac cccgcgatcc aatcagattg
     6481 gcgatcgtgg agtggaggtt tcgctgcgga gaatcgcccg atacgccgac ggctggatga
     6541 ccaatcagat cgagctcgaa aggtttaagg attacctcgg gcgattacgt ggcatgttgt
     6601 tggacgaggg gcgcgatccg aatgccttca agaccatttt gtattatggc atctcggtca
     6661 atgccgatcg cgctcacgct tttcgccaag caaaaacttt tcttgatgcc tactatcaaa
     6721 aggactttac gcgccagggc gttgaaatct ggaacgcctg cgggccggtg gaacactgcg
     6781 tcaactgtgt aggtgacttt atcgaggcgg gtgtcgacca cgtcaccatt cgcccgatcg
     6841 gcgacgattt aactgagcag ctccgtatct acttgaaaga ggttcttccc gccttgcgat
     6901 cgtaagctgc actcatgaaa cttatccatc agagaattcc cagctacaaa cctctcggca
     6961 gtatagaatc aagtggaggc tcggctttga cctcaccgcc aatcgaaagc ccgagcggca
     7021 aaacgatcgt gaacgttgtg cctttgccga tttcgctgcg gtatgtaatc gttcccttat
     7081 gatcgctaac gatctgctgc acaattggaa gtcccaagcc cgtgccctcg ggtttggtag
     7141 ttctgaacaa ctgaaataca tcgatgccgc gcgctatccc ctcacccgtg tccgtcactt
     7201 ccagaataat ccccccgtct gcagcactgg ccttgaccgt cagagttccg ccgttcggca
     7261 tcgcttcaac ggcgttcttg cagagattta aaatgacctg cttgattttt tcccgatcga
     7321 caatcgtgcc cggcacgttt tccaattgcg tttgaagcac gactttggtc gctcgatagg
     7381 tcggcaactg ggcactcagc acctcctcga taatctgcgt caaatcagcc gggcgcaggt
     7441 taagcctctg cggtcgcgca aacgtgcgat aatcggaaag caaggtcgcc agccgctgca
     7501 cttcctgaaa tgcgatgtcg agcgtttcct gtaaaaccgg atccgtcttg tcagcggttt
     7561 ctagaatcga tttgacaatt tgtaaggacg tggaaatacc attgagcgga ttaccgatct
     7621 catgggcgaa taccgcggca gttgtgccca acgtggcaag tcgctctttt tcctgaagca
     7681 atccttccgc ctgcttgcgt tccgtgatgt cgcgaatcgc cgcgctcatc agaacttccc
     7741 gccccgttgc aatcgggctt aagctgattt caaccgggaa ctcgctgccg tcgcggcgaa
     7801 cggcaaagag ctccatgccc gttcccatcg gccgaacgtg cggatttttc gaaaacatgc
     7861 cgcggtgatc ctgatgtcgc tcgcgaaacc gcgcgggaac aagtcgttcg accggcttgc
     7921 ccaaaagttc ttgtcgatca taaccgaaca gtttttcggt ttgtgaattt atcagcgtga
     7981 tcattccttg tgcatcgaca atgaccatcg cgtcgggcgc cgattctagc aaagagcgga
     8041 aaagcgtctc ggtttctctt aatttctgta cggccagttc tcgctccagg atttgttgtt
     8101 gcagttcctg attgaccagt tcgagcgagc gcgggctcgg aagcgccagc gcctgcggaa
     8161 tcagaggcca tagagtcgcg gccgttgcca ccgaaagacc ggcggtgatt gccttgatac
     8221 ttccatccaa ccagtagacc ggctcccaga gcttccatat tcccattaga tgtgtggtgc
     8281 cgcatgcaaa gatgaaagcg ctgaacatca ggaacatcca gttaaacgca agatctcgtc
     8341 gcttacgaac gaaaataatc agagcgatag gtatgatgta atacgaaatg gcaattaaag
     8401 aatcggacgc tacgtacaac cagagaagtg gtctctgcca gagaaaacag tggccgtgcg
     8461 gctcgaagcc ttcggacgta aagatgttgc ttaaaacttc gaacatagtc attccccccg
     8521 caggcagccc aaccggcgcg aacttactcg gctatgaaaa cgggcgatgg cttgttagtg
     8581 cgtaatttga attgtatcga gcagatcgcg caaagagatc ggcttttcga agtacagcgc
     8641 gcctactgct gcggcttctg cttgagcttg ctcggaaccg aacgcggtaa tgagaatgag
     8701 acgttttccc gaacccctac aactttgata cttaaggaca tcgagaccat tcaccttccc
     8761 gggcagacga aagtcagaga tcaccacgtc aaaattgact tgtgaaataa gctctaatgc
     8821 agcctcgcca ttgtcggtct ggtgaaccgt gaattcggct tgctgaagaa acatcgccaa
     8881 gttccggcgg gacaacgggt tgtcttcaac cagaagcaga ttacacgaca tgccgggaca
     8941 aattaaacca agattgtatt ttaagcaaca aaaacttcag cttaaggcgg caaattgcgc
     9001 tatagaaaca gcttctgtga ggctcaccta gcaccaatcg atagaaaatt ttgatcgatc
     9061 aagcgctgca gataaacagc gagctggcaa gtttctattt ggatgtacgc attattaagc
     9121 catcaggggc gtgtttgcgc gcttcgtgtc acttagcgag acgagtcctc tcggggaatc
     9181 gagcttcgct attcgagatc agatctatcc gtcgcgggtt acggcgtcgc gcgcaccgcg
     9241 ggccgttcga gcagacgctt catccaagct ttgacgtgag gcaagtcatc accgatgttt
     9301 acctgatatc tctcgcgtct aaggaagaat ggaatgtaag tgatatcggc gagagagtaa
     9361 tcatccgcta tgtactctcg cccttgcatt tgtcgattga gattttcgag atatgtccga
     9421 agctccttcc ccgactcttc gactttgtag ttgtgcgcga tataaccggc ggcgcgctgc
     9481 acgcgggtgt tgcagtagtc gatccagatt ctcacgcgcg cccgcgcgag ggggtctttg
     9541 ggcatcaacg gcacctcggg ataccgttct tctaaaaatt catcgataat tgcggactcg
     9601 taaacgaccg catcaccgtc gaccagcacc ggcactttgc cgtaaggatt tagttcgaga
     9661 agatctttag gcttgttgcc gaggtcgcat tcgatcaaat cataggaaat ctttttttcc
     9721 gccaggacaa gccttgtcct gtgagcgtag ggtcaggggc cgtaagagaa gagcttcata
     9781 agagtcacct ccgggtgatt gccttgtagc acctgagagc cgcggcggca acatttccga
     9841 ttgatcgaaa aagtttgcgg gtctaaacat aaagctcaag atcccatgcg cctcggatgc
     9901 aagcgatggt tgacactcgc ctgggctatg cgataagcgg cgatataacc attgattgtc
     9961 atatatactt tttcactcgc gtcatcgcgc ggacacttgg cgacgcctgt accggcgttt
    10021 ttgaagtgac ccgtttgcta gaggaggctg catgattatc gacacgcact ttcatgcctt
    10081 tcccaagaaa tttctcgacc tcgtgcccga gcaatcgcaa agcgatgttc gcggagtggg
    10141 attccacggc tttgaccatc aggagtatct cgatgtgatg gatcaacacg gcatcgatat
    10201 tggcgtgctt tcaaataccg gcggccgcat cgaaaaaggc ggcgaccggc gcaaagcgct
    10261 ggagctgtgc agaattatca acgatgcatt tgccgaagct cacgccaagc acccgcgccg
    10321 cttcaaagcg tttgcccggc cgccgatggt cgaccttgat gactgtgtcc gcgagctcga
    10381 acgttgttat cgtgaactca acatgcatgg cgtgatcctg tcgaccaatc tcgccggaaa
    10441 atacctcgat gagccggaat tcaagccttt ctgggatgcg cttgaagcag gcgcaaaacc
    10501 gctgtttctt catcccgcca atgcgccgtg ccaagccaat tggaacaagt actcgctgca
    10561 ccagaagctc ctttggccca ccgacagttc tttagcgata tcgcgaatcg tttatagcgg
    10621 gctattcgat cgctatccag ccatcaaact gatcgcctcg cacttgggcg gcatgattct
    10681 gctttacctc gaccggctca actggcgcga gggcaatgta gagtgccggg gagtgccgga
    10741 ggaatacttc aaaaagatct actacgatac cgccggtcca atccgcaccg aattcatcaa
    10801 actggtctac gatactgtgg gccctgatca aatcctgttc ggcgccgatt atccgcacgg
    10861 cagaggcggc cgcgatgatc agttctatcc gatgacttta gatgcgatgt ccaagctcga
    10921 tattcccgat agcgataaac agaagatcta ttatttgaat gcgcagagac ttgggttcta
    10981 accggttgct gataaagacc cctatgcttt gaaggatgag ttatgaaagc tgaaaattga
    11041 aatttccgct gtcagatttc caccttcagg cttgtaactg cctctggatc tttttgagca
    11101 gcctgtaaga gatttttcag caatctgaga aggaggactt ttatgagcaa tcgattggag
    11161 cacgtggcga tctattgcaa agatatcaac gaatcgatcg agttttaccg aacccacttc
    11221 ggcggccagc ctaccgctat cagaaaaggc gccgcgggct actcgttctg ttttgtgacc
    11281 atcgatggcg ccccgtcgat acagctgatg gaatcgtccg acaaaaccgg tgtgcaccat
    11341 tacggtttca tcagcgatga catcgagcaa gcggcaaaag aattcaaaga aaagaacgcg
    11401 agaatcctgc gcgaaaacag ggacccgtct ggcaaattga ctaccatctt cgtccaggat
    11461 ccgcacggac tcgagatgga ggtccgctcg gcgcgttagc agcgcgtgaa tgcatccgat
    11521 tcttttgccg ttggccttac agtcactaga cttacatcct ctgacatggg ccgggcttgt
    11581 tgcgacgctc atccagcgct gatgaggagg aaccatgttc ggaagtagct tcaaaaagca
    11641 cgcttttggc gccatcatcc tgagcgtgat ctgggcgacg actctcgttg aaccagctct
    11701 aggccaggca aaacacgaag acatcgcgat cggctatgca tccccgagcg gtgtcatgac
    11761 gccgctctac gtcgcccagg agcaaggctt gttcaaaaag catggcctgg gagtcaagga
    11821 gttgcttttg ctccgcggta ccgggccggc agcggcacag atgctggtcg ccgctacggc
    11881 gccgctcgcc gcgctcggcg gcgctctggt tgaagcggcg attcgcggcg ccgacgtcgc
    11941 gtatatcgcc agcacatcca atcacctgat cttctcgatt tattcacgtt cggaagtcgc
    12001 ccgtcccgaa gatctcaagg gaaaaaccgt cgccgtggat gccaagggcg gttccataga
    12061 actggcgacg atcatcgccc tgaaacagtt cggtctgact ctcggtaaag acgtgagctc
    12121 ggtttatctc ggcggtcccg tgccgcagtt aggtgctctt gaaaaaggca tcgtcgatgc
    12181 gacgactctc tcggcaccga caaccttacg ggctaggaag atgggctaca aagagttgat
    12241 cgacatcggc gggctcaagc tcagctatgt ccacaccgcg atcggcgtaa accgcgcctt
    12301 cgcaaagaat aatccggaaa gtgtcgagac ctttctcaag gcctatatcg aagcgatgaa
    12361 aatgacccgg gaacaaccgg atttcgcaat gaaagctatc gccaagtaca ctcagatcac
    12421 cgaccctgaa gcgctcaagg tcacttacga gacctttcta ccggcatttc aacaacgcgt
    12481 gccttatgtt gcgcgcgagt cggttcaagg agtgctgaac ttctccagtt cagcagaggc
    12541 taaggctcga cgcgccgaag attttatcga ccattctttc ttgaagaaga tcgaggaatc
    12601 atcttttgtt aagaagctct atggcgatgg ctagcccgaa tgaattcagg ttacacagca
    12661 gctaacgata gttgcaatga agctgaatcc gagaaccggc ggcccaagct ttaaattccg
    12721 tcctagcaaa attgaacgat cgtttcagtg tgagactccg aagcagcgag gataaccaat
    12781 gccaagagat atgcgcagtt ggatcaagca attggaagag acgggcgagc tcatccgtat
    12841 cgccaagccg gtgcacccgc acacgcaaat gggcgcgctg ttatatcaat cgcgtgaaaa
    12901 aggtttgctg tttgaaaatc tcgagggctt tccaggctgg cgcagcctgg gcatggcgcc
    12961 cgccaacttg cgccatgcgg cgcttgcttt tgataccagc attgagaaat tgattcccag
    13021 cgccgccgaa cgtactctca agcgacggcc ggtcgagctg gtctccaccg gtcccgtcaa
    13081 agaagtcatc ctcaagggag atcgggtcga tctgactaaa ttgccggcgc atgtagccgg
    13141 cagtgaagaa actccctaca tcgcctccgg attgatggtg gcccgggatc cggacagcgg
    13201 catccgcaat gtcgccttcc accggttgca gatcaaaggg ccgagaaaaa ccggagcttt
    13261 gatcgtgccg cggcacactc gggagattct caacaagcac gaggcgcgca atcaaccgat
    13321 gccggtttct atttttatcg gccatcatcc gctttactac atggcggcgg cgagcaccgg
    13381 tgcgttcgaa atggatgagc tggaattagc cggcggcctg atcggcgaat ccgccaaggt
    13441 cgtgaagtgc gagaccaacg acctcgaagt gcccttcgat gcagagatcg ttcttgaagg
    13501 gcgcattctg cctaacgtgc gtgaggacga ggggcctttt tcagaattcc atgattatta
    13561 cgttgccggc atgggaaaaa atccggtgat cgaaatcgat tgcatcacca gacgccacga
    13621 tgcgattttc aaagcgatcc agaacggctc ggaagtcgaa ggctgcatct ttcacaaagt
    13681 tcctttcggc gtcgcggtct acaaccacat caaaaacatc ggtggctatg tcaatctgca
    13741 caatctgctg gtgctaccgg gcttgttcgg gatcgtcgtg caaatgacgc cacgcttcta
    13801 tggcgaggcg aagcagattt tgatgggagt tctatcgagt ccgatcctgc acccgaaaat
    13861 cgccatcgcc gtcgacgaag atgtcaatat ttttaattac tgggaagtga tctgggcgat
    13921 caatacgcgc tgcaatccgc aagacgacat cgtcgtcatt cccggtaccc gcattcaccc
    13981 gatggatccg accggcttcg agccggtgcc gccgggaggg ccctactggc accgggtcgg
    14041 cggcaaggtg atcatcgatg ctaccaagcc tcccctttgc gcgggggaac aggcgcggca
    14101 accttttcag cggctaaagc ccaagggatg ggagaccgtt cgcctggaag acttcttgcc
    14161 tcagtgaatc acccggagcg atctgcgagg attaccagag gacgcgttat tccaaagccg
    14221 tcacctggat tccgatcgca gcattcgcga atgacggact ctaagttcag gatggaagaa
    14281 acggcgctgt gttcggttag ctctccggat cttttgcaaa acgagctgtg ttgatcactg
    14341 ctcgagcatg cgtgccatca ccgatcttga cattctgatc gttgatcgcc aacaccgcat
    14401 aacgcaactt gttgcccttc ttctccacaa gctcggcagt gaccgtaacg ctctggccga
    14461 tattcgtcgg cgctagatgc ctcacatcca cgtggatccc cactgtctgt tcattttctt
    14521 ccaggtaagg ctcggtcaat tcaacgcagg tcctttccat caggccaatc atcgctgggg
    14581 ttgaaaaaac atcgcgggca agctgtcgcg cccgcatctc caacgttgtc tggtaagtcg
    14641 ctgtacggga taaacccgga ttaagttggc ttttcatgac cgcaatataa catgcgggca
    14701 gcactcgggt agccgcccaa ctcacgagca ggaattcgag gctcagctgg tcgcctgatt
    14761 accttttcgg ctcgtagttt tgcggccgcg gacaaagctt gataacatcc gggcatggcc
    14821 tcggcggaaa agatcacggt catcctacga aacagcagct cgacgcttac caccaatgag
    14881 tttgaagtat ttgataaggt ctgtaatcgg ccggtcggaa cattcacgtt gaaaggtggc
    14941 gagtccagat cgattgaaat ctctcaggac gaaaccggca ggggcaactt gaagatacgc
    15001 aacccggatc tcggcccgaa cgattggctg gaagtctctt cggtcagtcg cggcgatgtc
    15061 gtcacagcat agagacgcat cacattctgc cgcacatacc ggcatccact gctcgtcgat
    15121 ttctaaggct caaaagtgat agccctctga cattcccgcc atgatcgcag ccaagcagcg
    15181 gatgccgcta tatcccgcga acgatcggtg agctacaatc ttttgttaac tgattttttt
    15241 agctgcccaa ttccattctc gatcaatctt ctctacacgt tgttcgtctg cgtgttagtg
    15301 ccgctctact ggcgccaata cggcccggca aattttcttt ggttttccga cctcgcgctg
    15361 tttatcgggc tggcggctct ctggctggaa agttccttgc tggcgagcat gcaagcggtt
    15421 tccgtgctcc tgctcgagct gctatggaat atcgagttct tcgtcggctt gatagccggc
    15481 aagcagatcc ttggcatggc cgggtacatg ttcaaagccg accagccatt atggctgcgc
    15541 gggttgtcgc tgtttcacgt tccactgccg gtgattttgc tttggcaggt ttgctgtttc
    15601 gggtacgact cgagggcctg gatcgtccaa accagtctgt tctggctggt catgccggta
    15661 tgctttttct tcacccgacc ggaagaaaat atcaattggg tgttcggtcc aggaactccg
    15721 ccgcaaagaa aaataccggc cggagtctat ttgctattgc tgctgattgg cgcgccgctg
    15781 ttcatctatt taccgacgca cgttctcctg cgcgcgatgc tattaaaatc acactgatca
    15841 tttattagcg cctttctatt ctccagccgg cgctgaaacg acatggactc tcgtccgtcc
    15901 ttgatctgga gtaaccgctc aagtccgcgg ccctgatgag ttcctgaaga atggcaccgc
    15961 agagacacag aggccgccga gaaaaacgtt tccgaactcg gtgcactccg cgcctcggcg
    16021 gtggatgagg tacgcgaacg gcagttctcc agctactgac tattagagct gttctttgag
    16081 gggaattgtg aacagttcga gaaacttttc gcgccagggc cgggccttcc agccttcgta
    16141 ggtcagtttc ttcgaatgct ttaagtcatc ggcgaacgcc ttctcgagct cgccgccaac
    16201 ggcgcgatcg taggcgatca ggttgatctc ctcgttcaga gcaaacgaac ggttgtccag
    16261 gttggtggtt ccaacatgcg cccagacgcc atccaccacc atggttttgg cgtgcatcaa
    16321 cgccgcttga tattcgtaaa tttgaatgcc gccgagcagc agaggctcga atcccctgcg
    16381 actggcccag tagaccagct tgtgatcgat ttttcccggc gtaagcacca ccacagacac
    16441 tcccctcttc acggcttcca aaagcgcctc ttgcatacgc tcatccggca aaaaatacgg
    16501 gttcgagaga tggatgcttt tgcgcgccga agtgatcgag agcagcaaga gcatgtagga
    16561 ttcgtaggtg ccgcccagag gcgagctttt aaccacctgg gcattgacct tgcccacggg
    16621 ttttagcgcc gggaaataaa gatcaccacc gagcacatgg ccggtggtat cccgccaact
    16681 ctccacgaac gcggcctgta gttgttggac aatggggcct tcgacccgaa cgtcagtatc
    16741 gcgccactta ccctcagtcc gcccgtcccc cgtccattct tcggcaactc cgtgaccacc
    16801 ggtaaaaccc aggatgccgt cgatcaccag aatacggcga tggttgcgat agttgtaatt
    16861 caacagctcc cacggggtga tgaattgaaa taatctgacg cgccgaaacc actcgagttg
    16921 acagccggca tcggttaaaa gctgaggaat gtcttcgggg atcttgctgc caccatggga
    16981 atcgagcaaa agtttcaccg ttaagcccgc gcggcagcgc tcggcaaatg cttcggcaag
    17041 ctcataggcg atcgcgccat cttgatagag atactgggca taggtaatcg atttccgcgc
    17101 accgcggatc gccttgagca tggctgggaa ggtttgctcg ccgtttagca gcacatcgat
    17161 tttgttaccg cccataatgg gcgcatcggt atgcgctgcg atggttggaa aaaatgttgg
    17221 ttcgccaacg cttatcgggt cgattttgct gatctgagta ggacccgcac aacccagaag
    17281 gaacatgtag atgaggaata gaatacgtat catctacatt caacgtaaca atttgttcaa
    17341 agcgcaacaa gcagcttcag cgagggtcat ggacgggcat cttccttgat atatctgcga
    17401 tcatcaagac ttaatacaaa atgtagcgcc taaaagttgt atcgggtccc gatgcctacc
    17461 ctgcaaggcc gggatcaagt acgagaagcc gcagactccg ccgccgtttt ctcttagcca
    17521 ttgtagcttt tactcgacga tagttgctta ttatgcgttt ttgccacttg acacaggcac
    17581 gagtgctcag aagactgctc aaaatcttag gaacttggga ccgcacatcg cgcgagccat
    17641 cacttgtcga tggcagacgc gttacgccgc ctcggcagat tagctactgc tccgctaact
    17701 gtctgatcgg ccgtccagaa aagtgcgggc tgaaaataag catccctttc ttgacattga
    17761 ctgccgccat tcctactgtt gatacagtag catcgcgacg gggtgtgaaa tgacaagggg
    17821 cattgcatac cgaatcgcat tcggacgata gactttattg cgctacggaa tgtgcccctt
    17881 agttgttgga ggcggcgatt aaaatggctg agcacgattg ggatttattc atctggtctc
    17941 gagcgaacga tttgctccat caagcagagc gcatccaacg caacttcata caaattgccg
    18001 cggcgacgca gtatcgctca tctcaggggc gagctccaag ctgggagcct ccggtcaacg
    18061 tgatcgaaac cgacgaaagc ctctgggtca tctctgccat tcccggagtg tcgtcggacc
    18121 aggtaaaagt acggatcgaa gccaacgaac tggttatcac cggccatcgg ccgctgccga
    18181 agtgttgcga cgatggtgat ctgaaaatat gggaaattcc gctcggccat ttcgagcggc
    18241 gtctcagggt catcgcaggc gaaacccggc tgattctcgg cgaggtcaca ttgaaggacg
    18301 gcctgctaat tattcagcta aggaaacatt catgaaagct gaaccggcgg aaactcccaa
    18361 gtccaaacct cggatcaaca tcccggaagg ggcaatggtc attttgccgc tgcgcaatgc
    18421 ggtgctcttt ccctcgacga tcatgccgct cgtggtcggt cgccccgcca gtctgcaggc
    18481 gatcgaagag accgtgcggc agcaattgcc ggtcggcttt ctcggtcagc gcgatccggc
    18541 agtggagaac ccaaggcccg aagatctcta caacatcggc acggcagcag acatacttag
    18601 gatgttcagc ctgccggatg gccagcggca aatcattgcc caaggacgcc gtcgcttcga
    18661 aatagccgag tttctccagg ttgatccctt gctgatcgcg cgggtcacaa tggttgaaga
    18721 gacaatcccg cagaccaaac agttcgaggc aagaatcgtt aacctgcgtc aagaagcgtc
    18781 cagagcacta tctttgttgc cggagccaat gaacgagctg aaaacgacca tcgagaggat
    18841 cgaagatcct ttgatgttgg tcgatatgat tgcctcgaca ctcgatataa caacggcgga
    18901 gaagcaggaa ctactcgcaa ctctggaccc ggtaacccgc gcccaaaaag tttcagaaaa
    18961 attggcgcgc caaatcgaag tcctggaact aagtaagaaa atcagcgaag aaaccaagga
    19021 ctcgatgacc aaggcgcagc gccagtattt tctccgtgag cagctaaagg cgattcaaaa
    19081 agagcttggt gaggaggacg gcaaaggggt agaggcggag gatctgcgcc agaagattta
    19141 cgatgcgaaa atgcctcccg acgtcgaaaa ggaagcactc aatgagctga accggttaca
    19201 gcgcatgccg gagggcggac ccgaatacgg ttcgctgcgc acctacttcg actggttggt
    19261 cgaactgcca tggagtgttc tgagcaaaga agaaatcgac ctgccgaagg cgcgtgagat
    19321 tctcgatgcc gaccattacg acctcgagaa agtaaaaaaa cgcatcatcg aatttcttgc
    19381 ggtccataag ctcatgcccg agggaaaaag tccgatcctg tgtttagtgg ggcctccggg
    19441 agtcggcaag acttccctcg gacaaagcgt cgcgcgagcg atgaatcgca aattcgtgcg
    19501 ccaaagcctg ggcggcgtgc atgacgaggc ggatatccgc ggccatcgcc gcacctatat
    19561 cggttctctg ccgggcaata tcattcaagg cattagaaag gccggcagca gaaatccggt
    19621 gttcatgctc gatgagatcg acaagctcag cgccagtttc cacggcgatc cttcggccgc
    19681 attgctcgaa gtcctggatc cctcacaaaa caacaccttt caggatcact atctcggtgt
    19741 gccttttgat ctgagccagg tgctgttcat cgccacggca aacgtcctcg ataccattcc
    19801 cggcccgctg cgcgatcgca tggaaattct ggaagtgccg ggctacatcg aagacgacaa
    19861 attagctatc gccaagggtt ttcttatccc gagacagacg gtcgagaatg ggctcaaacc
    19921 agaacagatt catttcaccg acgaggctat ccgggaaatc atcaggtcgt acactcgaga
    19981 ggccggcgtc cgtcaactcg agcgcgagct cggcgctgtt tgccggagcg tggcgacgcg
    20041 ggttgccgaa ggtcttacag agacgatcac cattgatgcc gaatcgatca ttagttatct
    20101 gggtcagaaa aagtttttta acgagattgc gctccgcacc agcttaccgg gtgtggccac
    20161 gggtttggcc tggacgcccg tgggcgggga tattttgttt gtcgaagtca ccaaaatgcc
    20221 gggagagggt cgattgattt taaccggcca gttgggcgat gtcatgaagg aaagcgcgca
    20281 ggccgctttg agtctgatca agtcacgcgc cgaagccctc ggcattgacc ccgaagtatt
    20341 taaaaagaac gacttgcaca tccatctgcc cgctggggca attcccaagg atggtcccag
    20401 tgccggcatc acgctctttg tcgctttggt ttcacttctc accggacggc gagtcagcaa
    20461 agatgtcgcc atgaccggcg aaatcagcct gcgcggcctc gtcctgccgg tcggaggaat
    20521 cagagaaaaa atgttggccg ccaaacgcgc cgggatcaca catgccttgt tgcccgacct
    20581 gaaccgaagg gatatcgaag aaattccact cgcggcccgc ggcgagatgc actttgattt
    20641 tctaaaaacc gtcgacgacg ctttattgct tgcgttggaa tccaacggcg ccggcagcgg
    20701 cttcgatgcc accgagcggc ccgcggccgg cgctgtaccc tgattgcgat tgtcctccag
    20761 cggggctgtt gcacgactca aactgctgga gtatcgaatt ttcactcgct tcgttagcgc
    20821 atccctgcac cctccaacaa tgtttaggta cgccctccat gatctaccgc ccgaagccct
    20881 caatcttcgc gcgactcatg atttgaactg cgcggcgact taaagatcgc aaaaccgccc
    20941 aacaggtcat tgcgccactc attggccctg gtaaactcaa cggcgaaatg ctgccatcag
    21001 tgctaggttc atcaaccatt gtaatagtcg atgcccagca acttcagcca acacccggga
    21061 ggaatcgatg cacaaaaaac tacgtctgtt attagccctg ggcgccgcct tgatcgtagc
    21121 gccggtttat gcccaggacc ccaaactcgt agaagccgga aaaaaagagg gcaaagttac
    21181 ggtgtacgga tcacttgaga ccgacagcgc agaggctgtt ttcaaggtct tcaggcaaaa
    21241 gaccggcatc gacctggagt actggagagc atcatcgacc aaagtcatgg atcgcgctct
    21301 gaccgagtat cgcgccggca aaccgggctt cgatgtaatt ttgaccaacg acaatccgat
    21361 gcagatcatg tcgagagaag gaatctttgc aaattacgaa tcgccttcgg ccaaagagtt
    21421 caccaaggaa tccatcgatc ccaaactcgg acctcgctac cgcaacgtca tgatcggcat
    21481 tctctataac aagagcatta tcaaacctgg cgcggagccc aagtcgctcg aagatctact
    21541 taagccgcaa taccggggaa agctggtcat ggccgatccg acccagcaca cgaccactac
    21601 tcagtgggtc gcgagcctgg aaaaattgct cggcaaggac aacgccgaga aattcattcg
    21661 cgacttggcc gcgaccaaac cgatcatggt cgagtctctc ttgcctgcgg cagagcgcgt
    21721 cgccagcggc gaaacacctg tcggcatcac ttatgtaaaa tacgttcata cgttcggtga
    21781 gagaggcgcg ccgatcgatt acgtcaggct cggcaagatg atgggcgacg gccattattt
    21841 gactcttggc aacaaggcgc cgcacccgaa cgccggcaaa gctttcatcg atttttttct
    21901 cggtcaggaa agcatgacga tgctcgctaa acagggcgaa ttcgtcaacc gcaaaggtgt
    21961 ctacccgccg attgccggcg ccgacaagat tc
//
LOCUS       DASUVS010000249         5313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_758146, whole genome shotgun sequence.
ACCESSION   DASUVS010000249 DASUVS010000000
VERSION     DASUVS010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758146"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VF182_13755"
     CDS             complement(<1..396)
                     /locus_tag="VF182_13755"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX6438198.1"
                     /translation="MVHMLLGFVVFVSGAVLMSLEIAGSRILAPHFGSSIFVWGSLIS
                     VVMASLSIGYYWGGWLSARDPSYGRLLTLLLIPGIVIFFLPFVYPAANEWVARNDFGM
                     RLNPLIACTILFLLPGVFLGTISPYAIRLA"
     gene            complement(400..2400)
                     /locus_tag="VF182_13760"
     CDS             complement(400..2400)
                     /locus_tag="VF182_13760"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX6438199.1"
                     /translation="MTTEYILKNPTEPIRNRSGLDYRKELNEAQYQAASTVEGPLLII
                     AGAGTGKTRTLVYRVAHLIDSGVDPRSILLLTFTRRAAEEMIRRAGLLIDSRCHQVAG
                     GTFHSFANLVLREYGRRIDLLPSFTIMDRTDSEAVIQEIRAEMGLNTKDRRFPRKQTC
                     AEIFSMALNKQTPLGDLIELEYPHLIESNEELIDLNRRYVEYKQTKSLVDYDDLLTKL
                     KDLLADHEQVRNRLSERFRFIMVDEYQDTNHLQSKIVRLLAATHDNVAVVGDDAQSIY
                     SFRGADFRNIMDFPKHFPGARIIKLEENYRSTQPILNLTNEIIQRAAEGYEKRLFTRK
                     NAGPRPRLVQAGSEQTQSQFVCQKILELREQGVPLWDVAVLFRSSFHSFDLEIELARQ
                     NIPFVKRGGFQFMEASHVKDLLAHLRILANPRDAISWTRVLLLLEGIGPGANQKIIKW
                     LLDGGNPIERLRTYPARGKIAQGLKTLAQVLEVTSEAELPAEQAQYLMQYYTPVLKHN
                     YPDDHPKRLRDLEHFQGMTERYRNLERLLSDMALEPPNDSIAGVLAVNPNEGPLILST
                     IHSAKGLEWNSVFIIWALEGRFPSIYNTNTTEELEEERRLLYVAATRAKQNLFISYPT
                     RVFDKSLRMVLSRPCQFVDGISHRLLEPVSLVPEGSLWSWES"
     gene            2582..3244
                     /locus_tag="VF182_13765"
     CDS             2582..3244
                     /locus_tag="VF182_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438200.1"
                     /translation="MIKPCAVLLLGLFLMVRCVSMLGAQEQPAIAEYRDPEYAYSFQY
                     PAHWKLKKLPEGAANDDIRVSLQTPGGDSFMVIVEKRGQNPTKAEFNSDPKRSGRVDK
                     LIDETTEEIYRIIARNLGAVETEFGEKTDLSNDAAIKFYVSSLNKMKAGEPIIVAGIH
                     LYPFSKPYSISFIMTAFFNPGAEKENALMRAVFNSFQLVEPAQPSSAPSSADLPKASQ
                     SK"
     gene            complement(3365..3742)
                     /locus_tag="VF182_13770"
     CDS             complement(3365..3742)
                     /locus_tag="VF182_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6438201.1"
                     /translation="MTSPTLLIVDDEFGVRESLKMVFCKEFRLMEADSVETAIPIVLE
                     SKPAIVLLDVLMPKTDGMEALRRIKEIEPACEVIMLTGVNSQQLSSKALNFGAFDFVA
                     KPFDVVDLRQKVARALRKTQEIK"
     gene            complement(3905..5068)
                     /locus_tag="VF182_13775"
     CDS             complement(3905..5068)
                     /locus_tag="VF182_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438202.1"
                     /translation="MLLLALKLRRALLWLAIFSTAQVGSSFAQAQPESPTSSELPSIE
                     ALKLCLAGPDSAACLDKIFRDVLKTESTKTALQLIQRFELQDPELRRDCHPIVHAVGR
                     ETFRIKGNIHDSFSACDQTCHSGCYHGSVERFLRGDDIYSQVERHPSQAELKEKAAAA
                     CDSDVPVRLRFQCLHGLGHALMFFSRYKLAPSLEACDALTEEWSRQSCYGGVFMENVS
                     SSTPELRDLSPTDYHYPCNRIDAKYRAECYVMQTSRMTEMGLSVSRVFEECQKSGEYD
                     LPCTLSIGRDLSNDVRMGQTHPTAQKCESVAGARRLACMRGVVYALIDNTWDGRYVLP
                     FCIAFEQDSDQQSCIKESIGYLKTTFQKSIEEITNDCAQYLGQPKRCLDLASR"
BASE COUNT         1277 a         1386 c         1413 g         1237 t
ORIGIN      
        1 cgccagccgg atcgcatagg gcgagatggt tccgagaaac acgccgggca ataaaaagag
       61 gatggtacaa gcaatcagcg gattcagacg catgccgaaa tcgttgcggg ctacccattc
      121 gtttgccgcg ggataaacga atggaagaaa gaagatcaca atgcccggaa tcagaagcag
      181 ggttaagagc cggccgtaag agggatcccg cgccgagagc cagccgcccc agtagtaacc
      241 gatgctcagg gaggccatca ccaccgaaat cagactgccc cagacaaaga tggagctgcc
      301 gaaatgaggg gcaagaatgc ggctgccggc gatctccagg gacatcaaaa ccgccccact
      361 aacgaacacc acgaaaccga gcaacatgtg caccatgaat taactctccc aactccaaag
      421 actcccctcg gggaccagcg agaccggttc gagcagccga tgcgagatgc cgtcgacgaa
      481 ctggcagggg cgagacaaca ccatgcgcaa gctcttgtca aagactctgg tcggataaga
      541 gataaaaaga ttctgcttgg ctcgggttgc cgccacgtat agcaaacgtc tttcttcctc
      601 caactcctcg gtcgtattgg tgttgtagat ggacgggaat cttccttcca gggcccaaat
      661 aataaaaacc gagttccact cgagcccttt ggcggaatga atggtcgaga ggatgagcgg
      721 accttcgttg ggattaaccg cgaggacccc ggcgatgctg tcattcggag gctccagcgc
      781 catgtcgctg agcagacgtt caagattgcg atagcgttcg gtcatgccct gaaagtgttc
      841 caggtcgcgc aaacgctttg gatgatcatc cgggtaattg tgcttcaaaa ccggcgtgta
      901 atattgcatt aaatattgcg cctgctcggc gggcagctcg gcctcggaag tgacttcaag
      961 gacttgcgcc aatgttttca aaccctgagc gatcttgcct ctagccggat aggtgcgtaa
     1021 ccgctcgatt ggattaccgc cgtcgagaag ccatttgata attttttgat ttgcccccgg
     1081 tccgatacct tcgagcagca gcagcacccg ggtccaggaa atcgcgtccc gcggatttgc
     1141 caaaatccgc aaatgcgcaa gcagatcctt gacgtgggat gcttccatga actggaaacc
     1201 gccgcgtttg acgaacggaa tgttttgccg cgccaattca atttcgagat cgaaggaatg
     1261 gaagctggag cgaaatagta ccgccacgtc ccataacggc acgccctgtt cgcgcaactc
     1321 caagatcttc tggcaaacaa actgtgattg tgtttgttcg ctgccggcct gcaccagccg
     1381 cggccttggg ccggcgtttt tgcgagtgaa caaacgcttc tcgtagcctt cggcggcgcg
     1441 ttggatgatt tcgttggtaa gattcaagat cggctgggtg ctgcggtagt tttcctcgag
     1501 cttgataatc cgggcgccgg gaaagtgctt cggaaaatcc atgatattgc gaaaatcggc
     1561 gccgcgaaac gaatagatgc tttgagcgtc gtcgcccacc acagcaacgt tgtcgtgggt
     1621 cgccgcgagg agccggacaa tcttcgattg caggtgattg gtgtcttgat attcatcgac
     1681 catgatgaat cggaaccgct ccgacagtcg gttgcggacc tgttcatgat cggccaacag
     1741 gtcctttagt ttggttaaaa gatcgtcata gtcgaccagc gatttggttt gcttgtattc
     1801 gacgtaacgt cgatttaaat cgatcagttc ttcgttggat tcgataagat gagggtattc
     1861 caattcaatg agatcgccca acggcgtctg tttgttaagc gccatgctga atatctcggc
     1921 gcaggtctgc ttgcgtggaa aacgccggtc tttggtgttt aaacccattt cagctcgaat
     1981 ctcttggatc acggcctcgc tatcggtgcg atccatgatc gtgaaagaag gcaatagatc
     2041 gatgcgccgc ccgtattcac ggagcacgag attggcaaaa gaatgaaatg ttccaccggc
     2101 tacctgatgg caacggctgt cgattaacag tccagcccgg cgaatcattt cttccgcggc
     2161 ccggcgagta aacgtcagca ataaaatcga acgtggatcg actccgctgt cgatcaagtg
     2221 agcgacgcgg tagaccagag tgcgagtctt tccggtgccg gcaccagcaa taatcagcag
     2281 cggcccctcg accgtgcttg ccgcctgata ttgcgcctcg tttagctctt tgcggtaatc
     2341 gagtcccgag cggttgcgga taggttccgt aggatttttg agaatgtatt ctgtggtcaa
     2401 gcaaactcca attcgaggta aattacctat tttggatcat atcggcaagc gatgatggac
     2461 aatgtttttg ctcctggaca tttagtcgct tcactgtaac cgtctcattc ctgccggtct
     2521 ttcatcgtcg gtcgcgtgtg ctagtctcag tctcgtaacg caatcgatgg gagcagaaat
     2581 tttgatcaaa ccatgcgcag ttttgttgct cggtttgttt ttgatggtgc gctgcgtgag
     2641 catgcttggc gcccaggaac aacccgcgat cgcagagtac cgcgatcctg agtatgccta
     2701 cagcttccag tatccggccc actggaagtt gaagaaattg ccggaaggag cggctaatga
     2761 tgacattcga gtctcgctgc aaacacccgg aggcgactcc ttcatggtga tcgtggagaa
     2821 gcgcggtcaa aatccgacga aagcggagtt taactccgat cccaagcgca gtggccgggt
     2881 cgataagctg atcgatgaga ctacggaaga aatctaccgt ataatcgcgc gaaatctcgg
     2941 cgctgtggag acagaattcg gtgagaagac cgatctctca aacgatgccg ctatcaagtt
     3001 ctatgtctcg agccttaaca agatgaaagc aggggaaccg atcattgtcg caggtataca
     3061 tttgtaccct ttttccaagc catattcgat cagctttata atgacggcct ttttcaatcc
     3121 cggcgctgaa aaggaaaacg ccctgatgag ggcagttttc aactcgttcc aattggtcga
     3181 gccggcgcag ccttcgagcg cgcctagttc tgccgatctt ccaaaagcca gtcagtcgaa
     3241 ataatcctcg gcaccttgaa actctctctg cttagaagcc tctgctgaag tcgaaggtta
     3301 gtcgattgtg aggcttgccc gggacgcagt ggatgatccg gcagatgagc cgcccaatca
     3361 gcctttattt gatttcctgg gtcttgcgca gcgctcgcgc gactttttga cgcagatcga
     3421 cgacgtcgaa tggtttagcg acgaagtcga aggctccaaa attcaaagct ttgctcgaaa
     3481 gttgctgtga gttcactccc gtgagcatga tcacttcaca ggcaggctcg atttccttga
     3541 tgcggcgcag agcttccatg ccatcggttt ttggcatcag cacgtcgagc aggacaatag
     3601 ccggtttgga ctccagcacg atcggtatcg ctgtctccac cgaatccgct tccatcaggc
     3661 gaaactcttt gcaaaaaacc atcttgaggg attcacgcac gccaaattca tcgtcgacaa
     3721 tgagcagagt cggtgaagtc atcggctaga gaaccacaaa ctctaactcc tgctcatcag
     3781 atgcccttgg gttaaccgtt ttcagagaca ccctcttgat cttgatcctg aacgactcga
     3841 tttacaccct gcaaagcgcc caagcagcgc atgctgttta gggctgcata tagtgaagca
     3901 ttcgctaacg tgatgccaga tccaggcagc gcttcggttg accaagatat tgtgcgcaat
     3961 cgttggtaat ctcctcgata gatttttgaa aagtggtttt caggtagccg atgctttcct
     4021 taatgcaact ctgttgatcg ctgtcctgct cgaaggcgat gcaaaacggc aacacataac
     4081 gaccatccca ggtgttgtcg attagagcat aaaccacgcc gcgcatgcac gccagccggc
     4141 gcgcgcctgc caccgactcg catttctgtg ccgtggggtg agtctggccc atccgaacat
     4201 cgttggaaag gtcgcgtccg atggaaagtg tgcacggcaa atcgtattca ccgctctttt
     4261 gacactcttc aaaaactctc gagaccgaca gccccatttc agtcatcctg gaggtttgca
     4321 tcacgtagca ttcggcccgg tacttggcat cgatccgatt acaaggataa tggtaatcgg
     4381 tggggctgag atcgcgtagc tccggtgtgg aactggagac attttccatg aacaccccgc
     4441 cgtagcacga ttggcggctc cattcttcgg taagggcatc gcatgcttcc agcgacggcg
     4501 cgagcttata tcgtgaaaag aacatcagag catggccgag accatgcaga cattgaaagc
     4561 gcaatcttac cggcacatca gagtcgcagg cggccgcggc tttctccttg agctccgcct
     4621 ggcttggatg gcgctcgact tgcgagtaaa tatcatctcc ccgtaaaaaa cgctccactg
     4681 atccgtggta gcaaccggaa tgacaggttt gatcgcaggc cgaaaaggaa tcgtggatgt
     4741 tacccttgat tcggaaggtt tcgcgaccca cggcatggac aatcggatgg caatcccggc
     4801 gcaactcagg atcctggagt tcaaagcgct ggatgagctg taaagcagtt ttcgtcgatt
     4861 ccgtttttaa gacgtcgcgg aaaatcttat ccaggcacgc agcgctgtcg gggccggcaa
     4921 ggcaaagttt cagcgcctcg atggaaggca gttcagatga cgttggggat tccggctgcg
     4981 cctgagcaaa gctcgaacca acttgagctg tggaaaaaat tgccaaccac aacaacgccc
     5041 ttcttagttt caatgcaagt aataacacaa tgactaatct cctttagagg attagaccgc
     5101 aaatataaca acggccatag ttgattgtta caagcgcttc tagcgagatc gcggcgcgtt
     5161 tttctttccg gctaaatgtg taactatttc acttatgatt cgtctaaagc gtaaaatgac
     5221 ccggcgattg cttgaactcc cttgtactat taaggcgtta gctttggcaa cgtttttcat
     5281 gacggcggcc cacggccagg aaccggtgaa ggt
//
LOCUS       DASUVS010000250        15651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1633059, whole genome shotgun sequence.
ACCESSION   DASUVS010000250 DASUVS010000000
VERSION     DASUVS010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 15651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15651
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633059"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VF182_13780"
     CDS             <1..226
                     /locus_tag="VF182_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438203.1"
                     /translation="VVALLYMLSVFGALLAKPWAWWTGLMAVVLNLLLVLPGLAYGAS
                     VLEVLAWSAVPVILLVYLFSPHRSNALRRA"
     gene            392..817
                     /locus_tag="VF182_13785"
     CDS             392..817
                     /locus_tag="VF182_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438204.1"
                     /translation="MTTKNLLGSVAIGAALFAMGCFLAIPVAIKYYQSTREYVATAEV
                     GVDADAVYRAAIKEAEARSSTIKITKRDDSGRLLEITDGLQSASFKAVSLNGQKAEIV
                     VVADVPKSTGGKAKEEELALRIIRIVADSLGARYEIAKK"
     gene            1098..1253
                     /locus_tag="VF182_13790"
     CDS             1098..1253
                     /locus_tag="VF182_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438205.1"
                     /translation="MVSGHDGYIGYKGRRMLSILNKQKLERVLAYLLDENEFLGSHGI
                     RSLNDDD"
     gene            complement(1417..2511)
                     /locus_tag="VF182_13795"
     CDS             complement(1417..2511)
                     /locus_tag="VF182_13795"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438206.1"
                     /translation="MMRMVGYLPKQRSWRINLASVMLSIVMFVSDCTSVLAASVAEIA
                     LYRGKDRETKLIEGAKKEGQLMFYNSNTWMEVVAQEFEKKYPFIKVGVWRSEGRDVLK
                     RVLEEYASGRYIADVVETSTGIEILQKRGFLQEHFSPEIDAYSDDVKEKGKSGGVFFW
                     ANRELYIGLGFNTKAVPANDAPKDYAALLDPKWKGKMTMAGGSSGPRWVGHIVNTMGR
                     EFLEKLSRQDVKVQNIVPAALANQVVSGEVPLSPTIFDSNMVTAKRKGAPVEWRPLEP
                     VLTNVGSSGMTTRSPHPHAAILFLDYLHSKEGQQVVMKGGLNSPRDDMDVDQKFKKNY
                     LENRYSLEEFEKHFAEWQDLMKRLFISGNK"
     gene            complement(2513..3382)
                     /locus_tag="VF182_13800"
     CDS             complement(2513..3382)
                     /locus_tag="VF182_13800"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6438207.1"
                     /translation="MWKERRIESLPRWPNGVRLPIIISPHHQSEEMSSLYPDDQPDGF
                     DFSERQYGGRRGAWRLLEIMEKHEVKGTWLICGATAEKYPEVSRAVKKAGNAIAGHTY
                     EHEMMCNLPAAKELELMKKTVTVFEDLLGERLRGWRTCFASHNTIDLILKHFDFEWDG
                     SMWNDDLPYLLEGHGRQLVEIPFAAYSDVALCSQRFQFNTYSTWNCTTPDFFLRGLKA
                     GFDALYERGAQEPVLMPLTVHDYVTGRPSRAKSFSDFIAYAKQFEGVVFTTHEDVVSW
                     WRDGTKTNLTGEK"
     gene            complement(3385..4233)
                     /locus_tag="VF182_13805"
     CDS             complement(3385..4233)
                     /locus_tag="VF182_13805"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6438208.1"
                     /translation="MAIEHRSVSEIKWPSGKRLPVVLTFEHQSGEGAPPLPGDRPNFM
                     VSGTMEYGGRTGIWKILELLDRFQIKATFFVCGTTAERYPQAIRAAHEAGHELAGMSY
                     SFEKVRGYSTEREQSVLRRSSQALENVAGIKIIGWRCPDYRTSPQTLDLLAENGFTWD
                     SSLLNDDLPYLFDCDGRQLVEIPFTTSTADKTFIGYPYPQRGGSDGLSNVWNNEFDML
                     YEESERASRLFILSLQTWGVGRPAPLRALKHFLERLSGHEELWFAKCSEIAQWSKRCL
                     QPKSGS"
     gene            4412..5242
                     /locus_tag="VF182_13810"
     CDS             4412..5242
                     /locus_tag="VF182_13810"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6438209.1"
                     /translation="MRPKSIEWPNKAKIAVSFFVGLESFVKYSQYRGSGDRVDYASVA
                     FGEYGGKVGIWRILDVFTKHQVKGTIDTNGLAAERYPDAVKELHSLGHEIVGHGWSND
                     VPPTALDDQEERRVIKRTVDAISSVTGKRPVGWVSPAHLMTDKTFGFLVDEGIFWSGD
                     LPGDDLPYHVNVESRPLVVMPRLDYANDLSLIFRPKNSPSLFFECFKTAFDRMYAEGD
                     DGSPKLIDALIHAHIGGRPNYIGMYEQCITYAKQFSDVWFCTKGEIAKWYLDCYVKKS
                     "
     gene            5357..7075
                     /locus_tag="VF182_13815"
     CDS             5357..7075
                     /locus_tag="VF182_13815"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6438210.1"
                     /translation="MVYDRLARWTGFNFNFQRILFFVLLTILCYLVLPPLFFTMQSSL
                     YVDRGFIEPELSLKNYTDFLTTPDTGVMLVNSLWFAFGSSLVALTVGTLLAWVVERTD
                     TPFKNLAYLSAFTSFAIPGIVKVIGWIMLFGPEKGVINVWLKELFDLQYSPFNIFTLS
                     GMILVEGVVWAPVVFLFMAAPFRSMDPSLEESALMSGAGPVQTFYHVTLKLALPSVLS
                     VLLLTFVRAMEAFEIPVLIGIPGRVLVLTSEIYLRLSSGLLPQYGMASAYGVVLIFLV
                     SIGLYFYSRATRESQKFYTITGKGFRPRTIEMGKWRVVTTMIVFVLPTLLALPLLILM
                     WTSVLPYYMAPSVDALRQLTTINYSAAFQSPNVWLALKNSLIISLTSASLTILLTALV
                     AWVVVRSNISGKGILEHLTSFTLAFPSVVLAVAMLLTYLKLPIPVYGTIWILVLGYIT
                     RYIPYGLRYCSPGLLQINKELEESAEMSGAPWFTVFRRIVVPLMMPSIFAGWIYIFLL
                     SVRELTMALLLYSPGSQVISVTIFKMWDFGTLGELSAFSVSVSLLLVGMAILFHRLSR
                     RYGLRV"
     gene            7100..8200
                     /locus_tag="VF182_13820"
     CDS             7100..8200
                     /locus_tag="VF182_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6438211.1"
                     /translation="MLRVRDLYKSFPTGLADVRAVDDIGFEVAHGKFFSLLGPSGCGK
                     TTTLRCIAGLERPGSGEIEVDGHLVFSQPGVFVPPYRRGIGMVFQSYAIWPHMNVFEN
                     VAFPLKIGHRASGREIKERVEKALHLVQLEDLGQRPATNLSGGQQQRLALARAVVREP
                     KLLLLDEPLSNLDAKLRDEMRVELKRVQRTLGLTTVYVTHDQTEALSMSDTVAVMNKG
                     KIVQLASPKEIYEKPLNNFVADFIGSANMIVGKLTALTSAEVGRVETKHGSLICAIPQ
                     PANTNVGIDVLVSVRPEDIQLTPAIESSNSSEWLGVIDQVSFLGGLIEYQIRISGTRM
                     RARVHPSNFFQEGDSVQLTFQPGRCCTVSSED"
     gene            8240..8974
                     /locus_tag="VF182_13825"
     CDS             8240..8974
                     /locus_tag="VF182_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6438212.1"
                     /translation="MDELAVLKDKLKTTVKILQWELCDMWGHVSAKTPDGKHFLLAFL
                     RPPADPKIPVDEVLEFDMSGTLLSGRRNVPDEIYFHLCPYKDRNDVGAVIHCHPPMSL
                     AVVATGRKIIPLYQHVAHFGNGVPISPWIYGTMRAHGNRAVKLMGENCALMIKGHGAV
                     VVGENIEEACMNMARMERTAKMMMYAAALGRPAPVPRGVGKMFAAIYRAPGNHKQEKK
                     LRSIGHKTEFQYYEGLVKRGEKWPTI"
     gene            9017..10006
                     /locus_tag="VF182_13830"
     CDS             9017..10006
                     /locus_tag="VF182_13830"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6438213.1"
                     /translation="MRMLMVGVGPIGGIIGGRLARAGHDIVFVDVDKEHVAAIRESGL
                     QVDVPDGSFNVRVRVGFPGETEGKFDIAFIAVRSNYTPSALSTALPRLADDGLLVSLQ
                     NGFNPPIMEGKVGADRTVGVAIRMGCKKIGPGQLQTSHRGHLYVGHLHRKTTPQLEEL
                     RKLLNSVIPTEISHEILDVLWSKLTHTCVCFFEALADVSLKQVCEKNSNLRVLVNYFA
                     EIIAVGKAAGAQFIPLPEYNPLDFHPIHSLEARLARMAEMARNFPENRKGPFRQIQRG
                     IVTEIDFIVGHVVREGERLEMPTPLCLKLAEMIHELESGKRQFDVENYAELSY"
     gene            complement(10283..11731)
                     /locus_tag="VF182_13835"
     CDS             complement(10283..11731)
                     /locus_tag="VF182_13835"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX6438214.1"
                     /translation="MNSLDLDRATISELSPYLETKKISPLELIKFVLAKTKKLNPVLN
                     AYVTVTEEVALADAKAAEREIQQGKYRGPLHGVPVSIKDNIATQGIRTTAGSKILSDW
                     MPNYDATVVDKLKAAGAIIIGKTNMHEWASGGTTINPYYGTTLNPWDTTRIPGGSSGG
                     SAAAVAAGLCLGSIGTDNAGSVRNPASFCGTIGLKATYGRVSRFGDVPGTGGFSTDHF
                     GIFTRTVKDGALVLAAVAGKDPKDPLSSKEVVPDYSSSLGKSISGLRVGILRGYAEDN
                     VSNEVRSAVENSVKVLQSLGMQRAEITVPHSDLIAAVQMVTSRVENVSNLDDYLRTRP
                     QDFSRSLLLRHIGSLMISGSTYVAAQRVRRVICEEFSQAFENLDVIVTPTTPLAAPKI
                     EECKTGYVEVDGRKIPYQNPTGSFGTVFTIPFNLTGLPAMSICCGYSSSGLPIGIQIV
                     APAFREAIMLQVAHAYEQAAGWYRTRPVIKQA"
     gene            complement(11735..11905)
                     /locus_tag="VF182_13840"
     CDS             complement(11735..11905)
                     /locus_tag="VF182_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438215.1"
                     /translation="MEEHVRRLNREYGFNLNEDEIKLVARQAEEAERLFQSLQEIDLT
                     HVMPILKVDSKG"
     gene            complement(11908..13056)
                     /locus_tag="VF182_13845"
     CDS             complement(11908..13056)
                     /locus_tag="VF182_13845"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438216.1"
                     /translation="MIDRSAAIGYKTGMRMHEKPKREPRSINPETRSPLVYAGISTTR
                     NPEKNTMTHCFRLVCVVSLFAISVGTAAAQDKLLVATLRSVSQACFWVALESGFYKDY
                     GLEVLPVNFSGGTQTITSLMSGDVQITTTGGSTAINAKLKGGDAVLISTTLGTFPYAF
                     YVSQRIRVADDLRGKKIGILSFGGTAYPATRFALQKLGLNPDTDVAFIQTGDQKARFG
                     ALISGSIDGTLIQPPDTLKAKELGFKPILDLAQSGIKFPMNHNSTTSKFLKTDRDKVK
                     RFMMGYIAGLARLKTDREFSEKVLAKYLGNPSKAILDETYDFWSEIFPPKPYVDREEV
                     ENYLATLKDRGSAKVEDFFDNSIVSELDREGFIDAVYKRKLKIAPKEK"
     gene            13159..14148
                     /locus_tag="VF182_13850"
     CDS             13159..14148
                     /locus_tag="VF182_13850"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438217.1"
                     /translation="MIIDMHAHFIPTEWIDALRKNGQACGCKIEQDSAGHLQISITGE
                     SKPLRLSPSLSDVPRRMRALNERRLDRQVLAPVMSMVGYRLPQRQAQGFSRLYNETNA
                     TFAETSNGRFIPVATVPMQFSQTAVEELDYAVNSLGIRMVEIGTNINGQNLDEESFRP
                     FFARAAELGVLVQLHPHQDQVAGKDRLGKYFLSNLIGNPMDTAIAAASLIFGGLLERY
                     PTLNVCLVHGGGALPYLLGRISCGYSQIPEIRTMPRVPEEYFQRFYFDTMTHDARGLT
                     FLHGLAGAEHLMVGTDYPYDNTGDQDPIGLLERAGISGSKSILGGNAAKLLNL"
     gene            14274..15290
                     /locus_tag="VF182_13855"
     CDS             14274..15290
                     /locus_tag="VF182_13855"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6438218.1"
                     /translation="MKSRYRTYATLVALSFLLLEMAMAWAQSETELTRGAKQEGKVMF
                     WSAMRNEDNQALVEGFEARYPFVKVDVLRASSEQLVNRAVTEALAGKGTADVLNGFAL
                     KALQNRGMVLAYESPEGTHYPAGFKDPKHHWISLYNGYSTIGYNSKLVSKADAPKDWP
                     DLLNPKWKGQIGMDKEEYFWYAGMLKHWGDEKGRTFMESLARQQIHWRSGHSLLANLM
                     STGEFPLMGIVYPDRIEQMKTNGEPVDWVRTADPILVNLGLVVIAAKAPHPNAAKLFY
                     NYSVSREGQTILRKRNRVSARDDVPPLVPDMDPKKLRLVPLDPEIATTTKYNEDFQKV
                     FGLR"
     gene            15304..>15651
                     /locus_tag="VF182_13860"
     CDS             15304..>15651
                     /locus_tag="VF182_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438219.1"
                     /translation="MRKIIFPWLDREFILLCGEARSSGTVEQQTDELFERFEAELRAS
                     QLSLEDTVRTRIFGADREARELSTSARAKILTGKRKAASSSYISTSHFDSNVRVALDL
                     LAMRPLRRGADREA"
BASE COUNT         3670 a         3928 c         4108 g         3945 t
ORIGIN      
        1 ggttgttgcg ctgttgtata tgctgtcggt tttcggcgcg ttgctggcaa aaccttgggc
       61 ctggtggacc ggtcttatgg ccgtcgtgct gaacctgctg ctcgttcttc ctggtctagc
      121 gtacggagct tctgtgttgg aagtgttggc ttggtcagcg gtgcccgtga ttttattggt
      181 ttatctattt tcgccgcaca ggagtaacgc actcaggagg gcctaacgtc cgcgagatga
      241 gcccgaaaac caaagtacat tatgtcattc cagtgattgt ctttagattc tcgctggatt
      301 ttcggtgtcc gcagccgcag tgattgtcag tagggtctat gcggcaccgt tgtcgatccc
      361 gattaaaagg agggttttgt atggaaagat tatgacgaca aaaaatcttt tagggtcagt
      421 ggcaatcggt gctgcgttgt ttgcgatggg ttgttttctc gccatcccgg tggcgattaa
      481 atattaccaa agcacgagag agtacgtggc aacggctgag gtcggtgtcg atgctgacgc
      541 agtttatcgc gccgccatca aggaagctga agccagatcc tcgacgataa aaattaccaa
      601 gcgggacgac tcaggacggc tcctcgagat taccgatggc ctgcagtccg cttcgttcaa
      661 ggcggtgtcg ttgaacgggc aaaaagccga aatcgtcgtg gtggccgacg ttccaaagag
      721 tacgggcggc aaggcaaaag aggaagaact cgctttacga ataattcgca tagtggccga
      781 cagtctcgga gcgagatacg aaattgcgaa gaagtaggtg gatttcctag ctgcaaatac
      841 aattagcttc catgagcttc gtagaaggga tgagccgagt gctggaataa ccggcggctt
      901 actgcaccaa ttattatcgt attagggtta ctcggctgta cagccgttgc agaaaattta
      961 cgctgccaat caacaatgac ggaaaagctc taggtcgatg aagaccttta cgggcgagaa
     1021 gcgcgagcca cttcagtccg tccttcgggc agcgacatgc tccgattttg attagcccat
     1081 cgtaacccac cgttttgatg gtcagcgggc atgacggcta catcggctat aaagggcggc
     1141 gcatgttgtc gattctcaac aagcagaagc tcgagcgcgt gctcgcctac ctgctcgacg
     1201 agaatgagtt tctcggatct catggcattc gttctctgaa cgatgacgat taacttatag
     1261 aaacatcgtt catactggct gggaaaggaa catatggaga aggcttacta tgaatcgaga
     1321 taatacgacc agcggtgtcg atactacctt gaatagcagg acgtagcctg acgttaatga
     1381 accggcggta accacccatt aaattgggag ctgctactat ttattaccgc tgataaacaa
     1441 gcgtttcatc aagtcctgcc actcggcaaa gtgcttctcg aactcttcca acgagtatcg
     1501 attttccaag tagttctttt tgaatttctg atcgacgtcc atgtcatctc tgggagaatt
     1561 gaggccgccc ttcatgacga cctgctgacc ctcctttgag tggagatagt cgagaaacaa
     1621 gatcgcggca tgcggatgcg gagatcgggt cgtcatgccg gatgatccca cattggtcag
     1681 caccggttcc aatggacgcc attccacagg ggcgcctttt cttttcgccg taaccatatt
     1741 cgaatcgaaa atggtcggag agagcggaac ttcacccgag accacctgat tggcaagtgc
     1801 agccggaacg atgttctgga ccttgacatc ctgtcggctg agtttctcca agaactcacg
     1861 gcccatggta tttacgatat gaccaaccca gcgcggaccg cttgagcctc cggccatggt
     1921 catcttgcct ttccacttgg gatcgaggag ggcggcgtag tctttcggtg cgtcatttgc
     1981 cggaaccgct tttgtgttaa atccaagccc aatatacagt tcgcgattcg cccagaagaa
     2041 aacaccgccg ctcttgcctt tctcctttac atcatcgctg taggcgtcga tctccggcga
     2101 aaagtgttcc tgcaaaaagc cacgcttttg taaaatttcg atgccggtgc tggtttcgac
     2161 aacgtcagcg atataacgtc ccgaagcata ttcttcgagg acccgcttaa ggacgtctct
     2221 gccttcgctc cgccatacac cgactttgat gaaggggtat tttttttcaa attcctgggc
     2281 gacgacctcc atccacgtgt tggagttgta gaacattaac tggccttctt ttttggcacc
     2341 ttcgatcagt ttcgtttctc gatctttgcc gcggtacaac gcgatctcgg cgacgctggc
     2401 ggcaagtacg ctagtgcagt cgcttacgaa cattacgatg gagagcatca ccgatgctag
     2461 atttatacgc caagatctct gtttcggtaa ataaccaacc atccgcatca ttctattttt
     2521 ctcccgtgag attggttttg gtgccatcac gccaccacga cacgacgtct tcatgggtcg
     2581 tgaatacgac gccttcgaac tgtttggcgt aagcaatgaa atcgctaaag ctcttcgctc
     2641 tcgatggccg gcctgttacg taatcatgga cggttaacgg catcagcaca ggctcttgtg
     2701 cgccgcgctc atagagcgcg tcgaaccccg cctttaaccc gcgcaagaaa aaatcgggag
     2761 tggtgcagtt ccaggtgcta taagtattga actgaaatcg ttgggagcat agagcaacgt
     2821 cgctatacgc cgcaaaaggg atttcgacaa gctgccggcc gtgtccttca agcaagtaag
     2881 gcaagtcgtc gttccacatg ctgccatccc attcgaagtc aaaatgtttc aagattaaat
     2941 cgatggtatt gtggctggca aagcaggtcc gccaaccgcg cagacgttcc cccaaaagat
     3001 cttcgaaaac cgttacggtt ttcttcatca gctcgagctc tttggcggcg ggcagattac
     3061 acatcatttc atgctcatag gtgtgacccg cgatcgcatt tccggctttc ttgacggccc
     3121 gcgacacttc cggatatttc tcggccgtgg cgccgcaaat gagccaggtt cctttgactt
     3181 catgcttctc cataatttcc agcagccgcc aagcgccgcg gcgtccgccg tattggcgct
     3241 cgctgaaatc gaaaccgtcg ggttgatcgt cgggatagag cgagctcatc tcctcggatt
     3301 gatggtgcgg gctgatgatg atcggcagcc gtactccatt cggccaccgc ggcagtgatt
     3361 caatgcggcg ttctttccac atggttagct cccggatttc ggctgcagac aacgtttgga
     3421 ccactgtgcg atctcgctgc actttgcaaa ccaaagctcc tcatggccgc ttaaacgttc
     3481 gagaaaatgt ttcaaagctc gcagcggggc cggacggccg acgccccagg tttgcaggct
     3541 caagatgaag agccgagaag ctcgttcgct ttcctcgtag agcatatcga actcattatt
     3601 ccatacattg ctcaagccgt cggagccgcc gcgttggggg tagggataac caatgaaggt
     3661 tttgtccgca gtgctggtcg tgaatggaat ttcaaccaat tgtcggccgt cgcaatcgaa
     3721 tagatagggc aaatcgtcgt tcaacagact gctatcccag gtaaaaccgt tttcggctag
     3781 aagatccaga gtctgcgggc tggtgcgata gtctggacaa cgccaaccga tgattttgat
     3841 gccggcgacg ttttctaaag cttgacttga gcgccgcagt accgactgtt cgcgctcagt
     3901 gctgtagcct cgcacctttt caaaactata actcatgccc gctagttcgt ggccagcctc
     3961 atgagcggcg cgaatagctt gtgggtacct ttccgccgtt gtgccgcaaa cgaaaaaagt
     4021 tgccttgatt tggaatcggt cgaggagttc caagattttc catatgcccg tgcggccacc
     4081 atactccatc gttcccgaca ccatgaagtt gggacgatcg ccaggaagag gaggggctcc
     4141 ttcgccggac tgatgctcaa aggtaaggac aactggaaga cgttttcccg aaggccattt
     4201 gatttcactc acagagcggt gttcgatagc caattgtgat cacctaacct gcgttttttg
     4261 ctgctataac aatcggtgtc agtcttgtca aacttacgag ttcatcgttt atatcgactt
     4321 acttgcggca gaaatacaat gctgatattt aagcgaaact gttccgccga aagcgctgcg
     4381 ctcaaactac accacgggta gaggaaacat catgagacct aagtcaatcg aatggccaaa
     4441 caaagcgaaa atcgccgtca gtttttttgt cggactcgaa tctttcgtca agtattcaca
     4501 ataccgggga agcggcgatc gggtcgatta tgcttcggta gcgttcggcg aatacggcgg
     4561 taaggtgggc atctggagaa ttctggacgt tttcactaaa caccaggtca agggcacgat
     4621 tgacacgaat gggcttgcgg ctgagcggta tcccgacgcg gtcaaagaac ttcatagcct
     4681 gggccatgaa atcgtcggtc atggttggtc caacgacgtc cctcctacgg cgctggacga
     4741 tcaagaggaa cgccgggtaa tcaagcggac agtggacgcg atctcgtcag tgaccggcaa
     4801 gcgtccggtt ggttgggtta gcccggcgca tttgatgacg gataagacgt ttggatttct
     4861 cgtcgatgag gggattttct ggagtggcga tttgcccgga gatgatctcc cgtatcacgt
     4921 taatgtcgag agccgcccgt tggtggttat gccgcgcctc gattacgcca atgacctaag
     4981 cctcattttc cgtccaaaga attctccgag tttgttcttc gaatgtttta agaccgcgtt
     5041 cgatcgaatg tatgccgaag gagacgatgg aagtccgaag ctgatcgatg ctctcataca
     5101 cgcgcatatt ggcggccggc ccaactatat cggcatgtac gagcagtgta tcacttatgc
     5161 caagcagttc tccgatgtct ggttctgtac taaaggtgag atcgccaagt ggtatctgga
     5221 ttgttacgtg aagaagagtt agtccgtcac tcggtcattg ttaatgaggc cgaacatttc
     5281 gtctgatcgc gacatactcc tggcagctgc cgcagcgctt cttgagcctg gcatcacatc
     5341 agcaacgatc taaacgatgg tttacgatcg tcttgcccgc tggactgggt ttaatttcaa
     5401 ttttcaacgt atactcttct tcgtcctgct gacgatactc tgctacttgg ttctaccacc
     5461 gctatttttc acgatgcaat cgagtctcta tgtcgatcgc ggcttcatcg agccggagct
     5521 atcgctaaag aactatacgg atttcctcac aacacccgac accggcgtca tgctggtgaa
     5581 ttctctttgg tttgcgtttg ggagttcact ggtcgccctt accgtaggaa ctcttcttgc
     5641 ctgggtggtc gagcgaaccg acacgccttt caagaaccta gcctaccttt cggcattcac
     5701 gtcatttgct attcccggca tcgtgaaagt tatcggctgg atcatgctct ttggtcctga
     5761 gaagggtgtt ataaatgttt ggttgaaaga actctttgac ctgcaatact ctccattcaa
     5821 catattcacg cttagcggaa tgatccttgt agagggggtg gtatgggcgc cggtcgtgtt
     5881 tttgtttatg gcggcgccgt ttcgttcaat ggatccatcg ttagaagagt cggcgctcat
     5941 gtctggcgca ggacctgtgc aaacgttcta ccacgtcact cttaaacttg cgctaccgag
     6001 cgtactttcg gtgcttctcc tgacatttgt ccgcgctatg gaagcattcg agattcctgt
     6061 cctcatcggt attcccgggc gcgtgttggt cctgaccagc gaaatttatc tgagactctc
     6121 aagcggactt ctgccgcaat atggcatggc gagcgcgtat ggagtcgttc taatcttttt
     6181 ggtttcaatc ggtctttatt tttattcgag agccactcgt gagtcgcaaa agttttatac
     6241 catcaccggc aaaggtttca ggccgcgaac catcgaaatg ggaaagtgga gagtcgtcac
     6301 gacaatgatt gttttcgtgc tgccgacgtt gctagctttg ccgttgctga tcctgatgtg
     6361 gacctcggtg cttccttatt acatggcgcc gtcggtagac gcgctccgcc agttgacgac
     6421 gatcaattat tcggcggcgt ttcaaagccc caatgtttgg ctggctctga aaaacagcct
     6481 gatcattagt ttaaccagtg cttctctgac aattctgctg accgcactgg tcgcgtgggt
     6541 ggtcgtcagg agcaatatct ctgggaaagg cattctggag cacctcacgt cgtttacgct
     6601 ggcctttccc agtgtggttc tcgcagtcgc gatgttgctg acctacctta aacttcccat
     6661 cccggtctat ggcaccatct ggattctcgt cctaggctac atcacgcgct acatcccgta
     6721 cgggcttcgc tactgctcgc ctggtctgtt gcagatcaat aaggagttgg aagagagcgc
     6781 ggagatgagc ggtgctcctt ggtttactgt ctttcgtcgc atcgtcgttc cgttgatgat
     6841 gccgtcaata tttgccggat ggatctatat attcttattg agcgttcgag agctcacgat
     6901 ggcgcttttg ctttacagtc cggggtcgca ggtgatttca gtaaccattt ttaaaatgtg
     6961 ggatttcggt actctgggcg aattgtcggc ttttagtgta tccgtaagtc tgctgcttgt
     7021 cggcatggcg atcctatttc atagattgag ccggcgttac gggctgcgtg tgtgatactg
     7081 aacgctcaag gtcgcgaata tgcttagggt aagggatctt tataaaagtt ttccaaccgg
     7141 tttggcggac gtgcgagcgg tcgatgatat cggctttgaa gtcgctcatg gcaaattttt
     7201 cagtctgctc ggccccagcg gttgcggaaa aactacaacc ctgcgctgca tcgccggact
     7261 cgaacggccg gggtcgggcg agattgaagt ggacgggcac ctggtttttt cgcaaccagg
     7321 cgtgttcgtg ccaccgtacc gtcgcggtat tggtatggtg tttcagtcgt atgcgatatg
     7381 gccccatatg aacgtcttcg agaacgtcgc ctttcccctg aaaatcggtc acagagcgtc
     7441 cggtcgtgaa ataaaagaac gggtagaaaa ggcgcttcac ctggtccagc tcgaggatct
     7501 cgggcaaaga ccggcgacca acctcagcgg cggccaacag caaagactcg ctcttgcaag
     7561 agcggttgtg agagagccta aactcttgct gctggacgag ccgcttagca acttggacgc
     7621 aaaactgcgc gatgaaatgc gtgtggagtt gaagcgggtg caacggaccc tgggtctgac
     7681 aacagtttat gtaacccatg accaaaccga ggcgctgtcc atgtcggaca ccgtggccgt
     7741 gatgaacaaa ggcaagatcg ttcagctggc ctctcccaag gaaatctacg aaaaaccgct
     7801 gaataatttc gttgccgatt ttattggatc cgccaatatg atcgtcggca agctgacagc
     7861 gttaaccagt gctgaagttg gtcgagtgga aacaaagcac ggttcactga tttgtgccat
     7921 cccgcaacct gcaaatacca acgtcggcat cgacgttctt gtgtctgttc gtccagagga
     7981 tattcagttg acgccggcga tcgaatcatc gaatagttcc gaatggctcg gagtcatcga
     8041 ccaggtgtca ttcctcggcg gcctaatcga gtatcaaatc agaatttcgg gaaccaggat
     8101 gcgcgcgcgc gtccatccgt ctaacttttt ccaagagggt gattctgtcc agctaacctt
     8161 ccagccggga cggtgctgta ccgtctctag cgaagattaa cgcacgtcct aaaaatgcgc
     8221 cggcatctag gagattaccg tggatgaact tgcggttttg aaagacaaac taaaaacgac
     8281 ggtgaaaatt ttgcaatggg aactctgcga catgtggggc catgtgagcg caaagacgcc
     8341 cgatgggaaa cactttctgc ttgctttcct gcgacctccg gcagatccaa aaatacccgt
     8401 cgatgaagtc ttggagttcg acatgagcgg cacgctatta tcgggccggc gcaacgtccc
     8461 cgatgaaatc tattttcacc tgtgtcctta caaggaccga aacgatgtcg gggctgttat
     8521 tcactgtcat cctcctatgt ctctcgcagt tgtcgccacc ggccgcaaaa tcattccgct
     8581 ataccaacat gtcgctcatt tcggcaacgg agtacccata tccccttgga tatacggaac
     8641 gatgcgcgcg catggaaatc gtgccgtcaa gttgatggga gagaactgcg cattgatgat
     8701 caaaggacat ggggccgtcg tagttggcga gaatatagaa gaggcgtgca tgaacatggc
     8761 gcgaatggaa cgcactgcga agatgatgat gtatgcagcc gcgctgggaa gaccggcacc
     8821 ggttccgcgc ggcgttggaa aaatgtttgc ggcgatttat cgggcgccgg gaaaccacaa
     8881 gcaagagaaa aagctgagaa gcattggtca caagactgaa ttccaatact atgaaggctt
     8941 ggtcaaaaga ggcgagaagt ggccgacgat ttgatgccat cgattagaca ttggatggca
     9001 aaggaaatgt gagcaaatgc gcatgttgat ggtgggcgtc ggacccattg gtggaataat
     9061 cggcgggcgg ctcgcgcgcg ccggacacga cattgtattc gtggatgtcg acaaagagca
     9121 tgtcgcggcg attcgggaaa gcggtttaca ggtggatgtt ccggacggat cattcaatgt
     9181 cagagttcgc gtcggttttc ccggcgagac cgaaggcaag ttcgatatcg cgtttattgc
     9241 ggtgagatcc aactatactc caagcgcact gtcaacggcg ctaccgcgcc ttgcggatga
     9301 cggtttgctc gtgtcgttgc agaacggctt caacccgcca atcatggaag gaaaggtagg
     9361 ggctgatcgg accgtcgggg tagcgatccg catgggttgt aaaaaaatcg gccccgggca
     9421 gctgcagacg tcacaccgcg gccatcttta cgttggtcac ctgcatcgca agacgacgcc
     9481 gcagttggaa gaactgcgca agcttctcaa ttctgtgata ccgaccgaga tctcacacga
     9541 aatattagac gtgctgtgga gcaaattgac ccacacctgt gtctgtttct tcgaggcgct
     9601 tgcagatgtc tccctcaagc aggtgtgcga aaagaattcg aatctccgtg tccttgtcaa
     9661 ctattttgct gaaatcatcg cggtcggcaa ggcagcaggc gcccaattca tcccgctgcc
     9721 tgaatacaat cctctagact ttcaccccat tcattccctc gaggcgcggc tcgccaggat
     9781 ggccgaaatg gcgaggaact ttcccgagaa tcgaaaaggc ccctttcgtc aaatacagcg
     9841 tggcattgtc actgaaatcg atttcatagt tggtcacgtc gtgagggaag gggagcgcct
     9901 ggaaatgccg acgccccttt gtctaaaact cgctgaaatg attcacgagc ttgaaagtgg
     9961 gaaacggcaa tttgatgtgg agaattatgc tgagcttagt tattgagggg atgggcgcaa
    10021 gtgggaatcg tccgagtcaa ggagcgcagt gaattggaac tggctgttgg agtgatcttg
    10081 tcggtgtttg tgcagttgtc gcttctcgct agctcgaacg cgagaggcgc gtaaaggaat
    10141 ccatttacga gacagcccgg ccgagattat ctgatccatc ttcaacctga gagtattctg
    10201 agatacgaca atcacgtcag caatcttcaa ggttgcgctg taggcgcatg taactgcata
    10261 gtgccgtcgc agatgcgaac gtctaggctt gctttatcac cggccgtgtt cggtaccatc
    10321 cagctgcttg ctcataggca tgtgctactt ggagcatgat tgcttcccga aaagctggag
    10381 ccacgatctg aattccgatc ggtaatccag acgacgaata accacagcag atactcattg
    10441 ctggaagccc ggttagatta aaaggtatgg taaatactgt gccaaagctt cctgttgggt
    10501 tttgataagg gatctttctg ccgtccactt ctacgtagcc cgttttgcat tcttcaatct
    10561 ttggggcggc taaaggcgtg gtcggtgtta ctattacatc caggttctca aaagcctgtg
    10621 aaaattcttc gcaaataact cgccttacac gttgagccgc aacgtaagtt gaaccggaaa
    10681 tcataagaga accgatatgg cgaagtaata aagatcgact gaaatcttgt ggacgcgtcc
    10741 ttaaatagtc atcgaggttt gaaacgttct cgacccggct tgtaaccatc tgaaccgctg
    10801 cgatcaaatc agaatgaggg acagtaatct ctgctctttg catacccagt gactgcaaaa
    10861 ccttaacgct attctcgaca gcgctcctga cctcattcga gacattatct tccgcataac
    10921 ctctcaaaat gccgactctt aaacctgaga tcgatttgcc caggctgctc gaataatctg
    10981 ggacaacttc ctttgagctc agcgggtcct tcgggtcttt tccagctacg gctgctagaa
    11041 cgagggcgcc gtctttaacg gttctggtaa agatcccgaa atgatcagta gaaaatccgc
    11101 cagttccggg aacgtctcca aatcggctca ctctaccgta ggttgcttta agcccgatgg
    11161 ttccacaaaa agatgcggga ttcctaaccg agcccgcgtt gtctgtgccg atagagccta
    11221 gacacagacc agccgcaaca gctgcggcag agccaccact cgaacctccc ggaattctcg
    11281 ttgtatccca aggatttaac gtggtgccgt agtacggatt gattgtggtg ccgccactgg
    11341 cccattcgtg catattggtt ttgccgataa tgatggcgcc cgccgccttt aacttgtcca
    11401 caactgttgc gtcgtaattc ggcatccaat ccgacagaat cttcgatccg gcggttgtcc
    11461 taattccttg agtcgctata ttgtctttga tggaaaccgg gactccatgg agcgggccgc
    11521 gatatttgcc ttgttggatc tctcgttccg cggctttggc atcagcgagc gcaacctctt
    11581 ccgtaaccgt gacataagca ttaagaaccg gattcagctt cttcgtcttg gctaatacaa
    11641 acttaatgag ctcaagaggg gagatcttct tggtttctaa atacggtgac aattcgctga
    11701 tcgtcgctcg atccagatct aatgagttca tctcttaccc tttactatcg accttcaaaa
    11761 tgggcatcac gtgagttaga tcaatctcct gcaagctctg aaacaaacgc tccgcctctt
    11821 ccgcctgtcg cgcaaccagc ttgatctcgt cctcattcaa attgaatccg tattcgcgat
    11881 tgagcctcct gacgtgctcc tccataatta tttctccttg ggcgctattt ttagtttcct
    11941 tttatacacc gcgtcgatga agccttcacg atccagctca gaaacaatag agttatcgaa
    12001 gaagtcttcg actttagctg aacccctgtc cttcaacgtt gcgagataat tttcgacttc
    12061 ctcgcgatcc acgtagggtt ttggcggaaa aatttcggac cagaaatcat aggtttcatc
    12121 caagatcgcc ttgctcggat ttcccaaata ctttgccaag accttctcgg agaactctct
    12181 atcggttttc aatcttgcaa ggccggcaat ataccccatc ataaatcttt tcaccttgtc
    12241 ccgatcagtc tttagaaact tgctcgtggt cgaattgtgg ttcatgggaa acttgattcc
    12301 gctctgcgcc agatcaagga tcggcttgaa cccaagctct ttcgccttca gtgtgtcggg
    12361 aggctgaata agagttccgt caatgctgcc gctaatcaag gctccgaaac gtgccttttg
    12421 atcaccggtt tggataaacg caacatcggt atcggggttg agacccagct tttgcaaagc
    12481 aaagcgcgtt gccggataag cagtaccgcc gaagctgagg attcctatct tcttgcccct
    12541 cagatcatct gcaacccgta tacgctggga tacgtaaaat gcataaggaa aggttcccaa
    12601 ggtggtgctg atcaaaaccg catcaccgcc cttgagcttt gcgtttatgg cggtggatcc
    12661 tccggtggtg gttatctgga cgtccccaga cattagagac gtgatagtct gtgttccgcc
    12721 gctaaaattt actggaagta cctcgaggcc gtagtcctta tagaagccgg actccaaagc
    12781 cacccaaaaa cacgcctggc taacactgcg aagggtcgcc actaacagtt tatcttgagc
    12841 tgctgccgtt ccgactgaaa ttgcgaacaa acttacaacg cagaccaatc tgaagcagtg
    12901 cgtcatcgta tttttttccg ggtttcgagt tgtagaaatt ccggcgtaaa cgaggggaga
    12961 acgtgtctca gggtttatcg agcgaggctc acgtttgggt ttttcgtgca tgcgcatgcc
    13021 ggttttatat ccaatcgccg ctgatctgtc tatcatcggt gtcagttaac tagcgattgc
    13081 ttgcatgtaa atcttgacat gcacagcaga gttgtgatca cgtccgacgt aagctgaatc
    13141 tgtgataccg aggaaatcat gatcatcgac atgcatgctc attttatccc taccgaatgg
    13201 attgatgcct tgcgcaaaaa tggtcaggcg tgtgggtgta agatcgaaca agactccgcc
    13261 ggtcacctgc agattagcat caccggggaa agtaaaccgc tccgattatc gccatctcta
    13321 tccgatgtac cgcggcgcat gcgggctttg aacgaacgtc ggctcgaccg tcaggttctc
    13381 gctccagtaa tgagcatggt cggctatcgt ctgccacagc gtcaagcgca agggttctcc
    13441 agactttaca acgagaccaa tgcaacattt gcagagacat ccaacggccg gttcatccca
    13501 gttgccactg tgcctatgca atttagccaa acggccgtcg aggaactcga ctacgcagtt
    13561 aacagtctag ggatccgcat ggttgagatt ggaacgaata ttaatgggca aaatttagac
    13621 gaagaatcct tccgcccgtt ctttgctcga gccgctgagc tgggtgttct cgtacaatta
    13681 catccacatc aggatcaggt cgcaggcaaa gaccggttag gcaaatactt tctaagtaat
    13741 ctgatcggaa acccgatgga taccgcaata gccgcggcat cgcttatttt tggcggtttg
    13801 ctggagcgat atccgacact gaacgtctgc ctcgtgcacg gaggcggcgc gctgccctac
    13861 cttctggggc ggataagctg cggttattct cagattccgg agatacgaac catgccgcgg
    13921 gttcctgagg aatactttca acgtttttat ttcgatacga tgacgcacga cgctcgcggg
    13981 cttacgtttt tgcatggttt agccggcgcc gagcatctta tggtcgggac agactatcct
    14041 tacgacaata ccggggatca agatccgatt ggattgttgg agcgcgccgg aatatcaggt
    14101 tcaaaaagca ttctaggtgg caacgctgct aagcttctta acctgtaggt ggtagtccgg
    14161 ccactatgac cacttgggca caacgaagcg catcggttta agcatgtcgc ttgggttaga
    14221 ttctctgatc agcattgaac gaggaatcga gcgaactcag aaaggagtgc atcatgaaat
    14281 cccgttacag aacatacgcg acattggttg ctttatcatt tctgttgctc gaaatggcga
    14341 tggcgtgggc acaaagcgag accgaactga ctcgaggagc aaagcaagag ggaaaagtga
    14401 tgttctggtc cgccatgagg aacgaggaca atcaggcgct ggtagaaggt ttcgaggcta
    14461 gatatccctt cgtcaaagtc gatgttcttc gcgccagcag cgagcagttg gttaatcgcg
    14521 ccgtcacaga agccctcgcg ggaaaaggga ctgctgacgt tctaaatggc tttgccttaa
    14581 aggctttgca gaatcgagga atggttttag catatgaatc cccggagggg actcattatc
    14641 cggcaggatt caaagaccct aaacaccatt ggatctcact ttataacggt tacagcacga
    14701 ttggttacaa cagcaagctc gtctctaaag cggacgcacc taaagattgg ccggaccttc
    14761 tcaaccccaa atggaagggg caaataggga tggataagga ggaatacttt tggtacgcgg
    14821 gaatgctcaa acattggggc gacgaaaaag gacgaacctt tatggagtca ctagcgcgcc
    14881 agcagatcca ctggcgatcg gggcacagtt tattagcaaa tcttatgtct acaggtgagt
    14941 ttccattaat ggggatcgtc tatcctgatc gcatcgagca gatgaagacc aatggcgagc
    15001 cagtcgattg ggtcagaacc gccgatccga tactggtaaa tcttggccta gtggtgattg
    15061 cggctaaggc accccatcca aatgctgcaa agcttttcta caactatagt gtgtcgaggg
    15121 aagggcaaac gattctccgc aagcggaatc gggtttccgc tagggatgat gtgccaccac
    15181 tggttccgga catggatccc aagaagttgc gcttggttcc gctcgatcct gagattgcaa
    15241 caacgacgaa atataacgag gacttccaaa aagtttttgg cttgagatag gattggatca
    15301 gttatgcgta aaatcatttt cccttggctg gacagagagt ttatattgct ttgcggtgag
    15361 gcgcggagtt caggcacagt cgagcaacag actgatgagt tgttcgagcg cttcgaagcg
    15421 gagcttagag cttcccaatt atctctggaa gacacggtac gtacgagaat tttcggggcg
    15481 gacagagagg cgcgcgagtt atcaacgtcc gcccgggcca agattctcac cggcaaacgt
    15541 aaagcggcaa gctccagtta tatttcgacg agccacttcg attccaatgt gcgagtggcc
    15601 cttgatttac ttgctatgag accgttgcgc cggggtgccg acagagaagc c
//
LOCUS       DASUVS010000251        10596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1633316, whole genome shotgun sequence.
ACCESSION   DASUVS010000251 DASUVS010000000
VERSION     DASUVS010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10596
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633316"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VF182_13865"
     CDS             <1..446
                     /locus_tag="VF182_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438220.1"
                     /translation="HGTLSVPQIIHTVRVPQPEIREDDEDENEFEDLSLTSFLTANAI
                     RSKHSLDESKIDWCSTNNSTPCAVANISVPTLVMAMQGHYFIRDGESIYESAASKDKQ
                     FVVVEGATHGLGNCNACADYHGTGPYTNVPLNLWNYVRDWANGRF"
     gene            complement(696..1289)
                     /locus_tag="VF182_13870"
     CDS             complement(696..1289)
                     /locus_tag="VF182_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX6438221.1"
                     /translation="MMMAALLSTAAGCGAISDESHRSISRGAVLPAASLNDNPSVDEP
                     QADIPRESSSVANIPRSPETQEDSPPSTVPTPTPSTIIGTGLASWYGAKHHGKRTASG
                     EIFDQKKFTAAHRTLPWGSIVKVTNLDNGKSVEVRINDRGPFSKGRIIDVSRAAARAL
                     GMVESGVSPVRMELLPPGETSNELVLQNSQSPSTEGN"
     gene            1828..2010
                     /locus_tag="VF182_13875"
     CDS             1828..2010
                     /locus_tag="VF182_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438222.1"
                     /translation="MPRNTQSSCTVLREPILGGCAIPPGCFSQGETVDEAINKFEMSS
                     NFMCRGKGREIPKKKI"
     gene            complement(2287..3321)
                     /locus_tag="VF182_13880"
     CDS             complement(2287..3321)
                     /locus_tag="VF182_13880"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438223.1"
                     /translation="MAGYRIGILAALFLGLVAFDHHRIELHAAAAPPKVVISFAGVNP
                     RQTPLWIAQEQKFFTKHGVDADVVFIRTGPIQVAAVSSGATQLAYSGPASVLGAAAGG
                     TDLRLIASFTNKLTYTMIARPEIKRAEDLRGKRFGVQAIGGSVWMGAVLGLEHLGLEQ
                     RRDNINIINVGDQTVLAQAMESGTIDVTVLDGVFSRRLKQKGFSVLVELAETTIPYVS
                     NTIVGTRTFFQQQPELTENIMKGLIESLAFIMAPQNKPIVVNSIMKRLKISDPSVAEQ
                     GYQDLVQISDPKPYVSLEGLRNIQRLIKIQNPPIGNVKLEELIDNRPLKKLEDSGFIQ
                     KTFAAYGVAR"
     gene            complement(3369..4406)
                     /locus_tag="VF182_13885"
     CDS             complement(3369..4406)
                     /locus_tag="VF182_13885"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438224.1"
                     /translation="MYIDCDSHYFPVKFLEPVSAKFPHSPRVVRKGDDVRSVLPDGTL
                     IKNQAPKGRWHLDTRDQHVDWEGFDVHVLIPENRELLYTWDRDLGCELARAYNDAVAQ
                     DLKECTHPEKYIGVAWVFLPDVEESCREVERAVKTLGLKAVKFMGGFEGANLGAEKLW
                     PFYDKIGQLDIPILVHDTASDRDGHPNPALVGIEQLFLEADNNALAFLLAFPFRYMVD
                     IGSLILRGALKEFPELKICFVEGSAAFVPWLMERLDMDIKAKERLGKLPSEFFNQIWV
                     SAFAAETSLKYACDFWKDHNLTVGSDYPHGDPSATWNIGTVKMIKEMDGMSEVDKEKI
                     LGGNAMRLFGM"
     gene            5076..5393
                     /locus_tag="VF182_13890"
     CDS             5076..5393
                     /locus_tag="VF182_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438225.1"
                     /translation="MKKPNAKSSRLNRDASDKEIIRWAKSNDVFDRLEAGISEIIENH
                     TDLNAILYEAIFQDNTAQLNMRLPPAMKAVLSKLARERTTDATTLARIWLAERLEREL
                     KAG"
     gene            5774..6481
                     /locus_tag="VF182_13895"
     CDS             5774..6481
                     /locus_tag="VF182_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX6438226.1"
                     /translation="MLHIEQARLVRHMQREAIVERLGKLDTCSVSDGLDSLKLAGATW
                     GVRPQWLCSRIAGRAVTVKIKPVGLQQPTQHLGTPAIEAAQPGDIIVIDNGGQRQFST
                     WGGLLSLSAKLKGLSGVVIDGACRDIDEARDLQFPVYARGVVPMTARGRVMQDSFNQE
                     IQFAGVQVHPGDLVIADGSGVVFIPKEKEEEVLDAAEMVFRKEAQMAEGIRKGSSSLE
                     MLEKLGYERMLQADKTK"
     gene            6687..7325
                     /locus_tag="VF182_13900"
     CDS             6687..7325
                     /locus_tag="VF182_13900"
                     /inference="COORDINATES: protein motif:HMM:NF006731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HEX6438227.1"
                     /translation="MASSLDETVISRAIHFGTATLHEAMGRKGALPHRIKPISPRMRV
                     AGLAVTVSSPPMDNLMLHQAIYAAEPGSIIVAEVNRGYEGGYWGEIMTIAAQQRKIAG
                     LVIDGCVRDADLIEHLGFPVFSRGLSVRGTDKQGGGHINWPILLGDITINPSDLVVGD
                     RDGVIVIPAAEIAATLEAAQKREAKEDQTKRDLMAGKSTLGIYGWPPPKLNS"
     gene            7810..8679
                     /locus_tag="VF182_13905"
     CDS             7810..8679
                     /locus_tag="VF182_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase"
                     /protein_id="HEX6438228.1"
                     /translation="MRRGEFDEAWRNSDILLRHHLAAPRDAHDVPRHQQVIWNGASLQ
                     GKRVLVRCYHGLGDTIQFIRYIPLLKAIARHVTVWAQPSLVTLLSTACGIDRMFPLHD
                     GTPDCDYEADVEVMELPYVFRTTIETIPAQVPYFHLDASALEDCRMKVGLVWRGGDWD
                     QRRDVPFDLLTQLAELQEISFYALQQETSAFEHHQRLKMILPAGADALTTARVMLALD
                     LVISIDSMPAHLAGALGIRSWTLLQKNADWRWMSDRTDSPWYPTMRLFRQEQAGDWEP
                     VVAQVKRELRKLL"
     gene            8956..10044
                     /locus_tag="VF182_13910"
     CDS             8956..10044
                     /locus_tag="VF182_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia lyase-activating protein"
                     /protein_id="HEX6438229.1"
                     /translation="MIPSKQATLTDESTYQQWVRTEGIPIIREFFIQDITKVALEPWD
                     RMGGSGVYLNLIGTGEANDAYILEISPGQKGREQHHLFEEMIYVIGGRGATSIWQANG
                     KKQTFEWQTGSLFSPPINCHYQHFNGSGSEPARMLAVTNAPVLLNLFHNADFVFNNSY
                     VFSDRFSGEEEYFSGKGESFPGRIWDTNFVPDVHTMALQSWKERGAGGKNIMLELSEN
                     TMGAHISEFPVGTYKKAHRHGPGAHVIIIAGKGYSCLWPQGEEIKRFDWQPGSIVVPP
                     EFWFHQHFNAGNEPARYLALRASGKKFRGLTKLYGVDEDVKKGGSQMEYEDEDPQIHK
                     EFEQELEKVGARCAMGGQHPRCTEKAAA"
     gene            complement(10263..>10596)
                     /locus_tag="VF182_13915"
     CDS             complement(10263..>10596)
                     /locus_tag="VF182_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438230.1"
                     /translation="LVGATDAIWSAARGTILQLTTPERFRGRIMGVFQLSNRGLQPLG
                     QTETGLMVPLIGARETTVVGGLLVSAVTLMIAWRIPSIIRFRWNEGEDRDGREAQGNK
                     VADRQNDG"
BASE COUNT         2451 a         2799 c         2969 g         2377 t
ORIGIN      
        1 agcacgggac cctctctgtt ccccagatca ttcacacggt ccgagttcct cagccagaga
       61 tcagggagga tgatgaagat gaaaacgagt ttgaagattt atccctcact tcgttcttga
      121 ccgccaatgc catccgatcc aagcactcgc tggatgagag caaaatcgat tggtgtagca
      181 ctaacaactc tacaccgtgc gcggtcgcga atatttcggt gccaaccctg gttatggcca
      241 tgcaggggca ttacttcatt cgtgacggtg aatctatcta tgaaagtgcg gcaagcaaag
      301 ataagcagtt tgtggtagtg gaaggagcga cccacgggct tggcaactgc aatgcgtgcg
      361 cagactacca tgggactggc ccttacacga acgttccgct gaatctttgg aattacgtgc
      421 gcgattgggc gaacggtcgg ttttagcgag aactgttagc agttgaccgg cttggatggt
      481 ttcgccggaa actaaacggc cggacgtttc actctaacgt cagccctttt ctagctatct
      541 gtgaagtcga gagattccgg tacgtagcct ggttagctgg cttggtcgtc gcggaaaaag
      601 cgagcgcaac gataaccgag gcgctttctg gcggatgcag gttgacttgg actcggcgat
      661 gcctgaatga tcgaagatct gtaacgatga gggggctaat taccctctgt tgaaggcgat
      721 tggctattct gcagcaccag ttcgtttgac gtttcgccag gcggaagcaa ttccattcgc
      781 accgggctaa ctcccgattc caccatgccc agagcccttg ccgcggcgcg cgagacatcg
      841 atgatgcggc ccttagaaaa cggcccgcga tcgtttatcc gcacttcaac tgattttcca
      901 ttgtcgagat tggttacctt cacgatgctg ccccacggaa gagttcgatg cgcggcggtg
      961 aatttcttct ggtcgaaaat ttcgccgctc gcggtccgtt tgccgtgatg ttttgctccg
     1021 taccacgacg ctagcccggt ccctatgatc gtcgaaggcg taggcgtcgg gacagttgac
     1081 ggcggcgaat cctcctgggt ttcgggagag cgcgggatat tcgctacaga tgaactctca
     1141 cgcggaatgt cagcctgtgg ctcgtccacc gagggattgt catttagact cgcagccggc
     1201 aacactgcgc ccctcgatat cgatcgatga gattcatctg aaatggcgcc gcagcctgca
     1261 gcggtcgaca acagcgcggc catcatcatc catgcgcgta tccgcgactc aaagcaggac
     1321 agatgactcg gcaaagttga tgatcttgta aacgaacggt gcatgactca ccgcgaaact
     1381 agttccgcaa tgaccatgcc cctgaatcac tgatggacac cggcggcggg gaggcgcaaa
     1441 agcccgcgat agggcggatt aactgacaaa aacgcaccca aaatcgggtg ggttcgtacg
     1501 acatcgtacg aaaatgatgg ccttcagccc ggcaacgaat atgaacaaag agcgtgaaat
     1561 cgggaattga gcattggaat cgagggttag gttttcatca tgactgtcaa atcacacggc
     1621 cacgaaggct gtgacgacgc tgcaccgtct gtatgcggga tgctttacac gctgcgaaga
     1681 tgaatatttg agagttcacg atgaaaatgc cgcgtggaca gctcattaca attccatgca
     1741 taggagtgaa aatcgctttg cttgagagcc tatccgcaag cacggttgtc cgagcaacag
     1801 ttcctggact ggctctggaa agcgaagatg ccgcgcaata cacagtcgtc gtgcaccgtg
     1861 ctgagggagc cgatattggg tggatgtgcc atccctcccg gctgtttcag tcagggtgaa
     1921 acggtcgacg aggcgatcaa caagttcgag atgtcatcga acttcatgtg caggggcaag
     1981 gggcgcgaaa ttcccaagaa gaagatttga tgattgcccg agtagacgtt tgaacagctc
     2041 gagcatggag ccctggtgca aagtcgcaga cggagagaag aagttcggga aggaaggtca
     2101 tttaaccttc acgagctcgc catcgcataa gctaagagag gatgagcgat cgaatgaatc
     2161 acgctctgga atcgatcgtt gtttactgtg aacgcgatcg ctatagacgc agttctgcga
     2221 tgttcggatc cgcgggtcga accctaacac cgaagtttct ctccattctt cattctccct
     2281 gtctagctat cttgcgactc cgtaggcggc aaaagtcttt tgaataaaac cgctgtcttc
     2341 gagtttcttc agcggccggt tgtcgattag ttcctccagt ttaacattgc cgatgggcgg
     2401 attttggatt ttgatcagcc gctgtatatt gcgcaagcct tccaaggaga cgtacggctt
     2461 tgggtcggag atctgaacca ggtcctgata tccctgctcg gccaccgacg gatcgctgat
     2521 tttcagacgt ttcatgatag aattcacgac gattggtttg ttctgtggcg ccataataaa
     2581 tgccaaactt tcaattaatc ctttcatgat gttctcggtc agttccggct gctgttggaa
     2641 aaatgtccgg gttccgacga tcgtattact gacataggga atggtcgtct cggcaagctc
     2701 gaccaggacg gagaagcctt tctgctttaa cctgcggctg aaaacgccgt cgagcacggt
     2761 gacatcgatg gttccgctct ccatcgcctg cgccagcacc gtctgatcgc cgacgttgat
     2821 gatgttgatg ttatcgcgcc gctgctcgag gccgaggtgt tcgagcccca agactgctcc
     2881 catccaaaca gatccgccga tggcttgcac gccgaaacgt ttgccgcgaa ggtcttcggc
     2941 ccttttgatt tctggccgcg cgatcattgt gtaagtaagc ttgttggtga aagaagcaat
     3001 cagccggaga tcggttccac cggcggctgc gcccagcacc gatgccggac cggaataggc
     3061 aagttgtgtg gcgccggagc ttacggctgc cacttgaatc ggtcccgtgc ggataaagac
     3121 gacatccgcg tcgacgccgt gcttggtgaa aaatttttgt tcttgcgcga tccagagcgg
     3181 agtttgcctg gggttcacgc cggcaaacga gatgacaact ttgggaggcg cagccgcagc
     3241 gtgtagctcg atccgatggt gatcaaatgc caccagccca agaaacagag ccgccagaat
     3301 accgatccta taaccggcca tagctcgcct ccttgaccgc ccatcgactg gcgcggaatt
     3361 ctcccccgct acatgccaaa taagcgcatg gcattaccgc cgaggatttt ttccttgtcg
     3421 acctccgaca ttccgtccat ctcttttatc atcttgacgg tgcctatgtt ccaggttgcc
     3481 gatggatcgc cgtgcggata atcgctgccc acagtcaggt tatggtcttt ccagaaatcg
     3541 cacgcatatt ttagcgaagt ctcagcggcg aatgccgaaa cccagatttg gttgaagaat
     3601 tcgctcggca gcttgccgag ccgttccttc gctttgatat ccatatcgag ccgttccatc
     3661 aaccagggaa caaacgccgc cgatccttcg acgaagcaga ttttgagctc gggaaactcc
     3721 ttcagcgcgc cgcgcagaat cagactgccg atatcgacca tgtagcggaa cgggaaggcg
     3781 agcagaaaag ccagagcatt attgtccgcc tccagaaata actgctctat gccgacgagc
     3841 gcgggattgg gatggccatc gcggtcggac gcagtatcgt gaacgagaat cggaatatcg
     3901 agctggccga tcttatcgta gaagggccag agcttctccg cgcccaggtt ggccccttca
     3961 aatccgccca tgaacttgac tgctttaagt ccgagcgttt ttaccgctct ttcaacctcg
     4021 cggcaggatt cctcgacatc cggcagaaac acccaggcga caccgatgta tttttccgga
     4081 tgcgtgcatt ccttaaggtc ttgtgccacg gcgtcgttat aggcgcgggc gagctcgcaa
     4141 ccgagatcgc gatcccaggt gtagagcagc tcgcggtttt cgggaatcag aacgtgaacg
     4201 tcaaagccct cccagtcgac atgttggtcg cgggtatcga gatgccagcg gcctttcggc
     4261 gcctggtttt taattaaagt tccgtcgggc agaaccgatc tgacatcgtc gcccttgcga
     4321 accacgcgcg gagagtgcgg gaatttggcg ctcaccggct cgagaaattt aaccggaaaa
     4381 taatgcgagt cgcagtcgat atacatggat ggaacctccc ttcgaagcct ccgacgcgcc
     4441 ggatcaagcg agtctgagat tgcacattag gcgcgctcag ggagggccgt caaccggaaa
     4501 cttcagggcg tggtagttca gccaagatct caccttaact gatcagcgaa aatgtcaccg
     4561 ctgggcatag tcaggtaaga gccagctttg cttgtgacat ctccatcagg gttctcgcgg
     4621 ccgtaacgct gcggtccgcc ggcatgtcaa gcatcattcc tgaaagccgc actgcattat
     4681 tattgcaagg tggcccgctg gcaaaaactc cagatccggt cgaatcgatt tttgtggtga
     4741 tttttttgct ttgctataag aggataatcg cccttaccgc aaggtttgcg atggcgtata
     4801 gcaactgcta caatccgcgc gatgggagtg attttttccc cttccgcgtt ccggcatggc
     4861 tatgccgaac aagatttata tgaattattg gcgacaaact atttaaaaat caaaagccag
     4921 cgcggactca atgatgtcta tgagttactc ggaagaaatc taagtggaac ctatcttcat
     4981 gtgatctatc gggtgttgcc cgatcgtacg ttacgggcat tccatataaa ttctatgaca
     5041 gaagcacaga aaaggcgtta tcggaggtac cggaaatgaa aaaacctaac gcaaagagtt
     5101 cccggttaaa tagggacgct agcgacaaag aaattatccg ctgggcaaaa tccaatgatg
     5161 tatttgatcg tttggaagcc ggcatatctg aaataattga aaaccatacc gatttgaacg
     5221 cgattctcta tgaagcgatc tttcaggaca ataccgccca gctgaacatg cggcttccac
     5281 cggcgatgaa ggcggtactc agcaagctcg cgcgcgaacg caccacggat gcaacgactc
     5341 ttgctcgtat ttggctggcc gagcgtttgg agcgggaatt aaaagctgga taggcaatag
     5401 gctgcgccgg gcgcgagtac ttgttcgaca attttcttct ctatgttgac cccgcaccgc
     5461 caggggaggt ggatatttca ggtttcgcca ttttccctac gtttcagaat cgaaaccgaa
     5521 gtttttcctg cttgtttcca cctcatccac ttcaaggccg cgcaccgcat tcagtgatct
     5581 cgggtggaac ggatgaaatg atttccgaat tgcggagaga aaattcgtat ccgatggtaa
     5641 acaccgctaa acgatgcgcg gtttgatcgc tctccccgct tgacacactc cgagctttcc
     5701 agatagatct ctcgtagata agtgagcggc ttaaagtcaa acccgctagc tacagcgagg
     5761 ttccggatga gctatgcttc acatcgaaca agctcgcttg gtgagacaca tgcagcgaga
     5821 agcgatcgtc gaacgactgg ggaagctgga tacctgctcg gtttcggatg ggttggacag
     5881 tttgaaactg gccggcgcga catggggagt acggccgcaa tggctgtgct cgcgcatcgc
     5941 cgggcgcgca gtgacagtta agataaagcc cgtgggttta cagcagccga cccagcatct
     6001 cggcacgccg gccatcgaag cggcccagcc gggcgatatt atcgtcatcg acaatggcgg
     6061 ccagcggcag ttttccacct ggggcggcct attgtcgttg tcggcgaagt taaaagggct
     6121 cagcggagtc gtgatcgacg gcgcttgtcg agacatcgat gaggcgcgcg acctgcagtt
     6181 tcctgtttat gcccgcggcg tggtgccgat gacggcgcgc ggacgggtga tgcaggattc
     6241 tttcaatcag gaaattcaat tcgccggtgt gcaggttcat cccggcgatc tggtgatcgc
     6301 cgacggtagc ggtgttgtgt tcattccaaa ggaaaaagaa gaagaggtgc tcgacgcggc
     6361 ggagatggtt tttcgcaagg aagctcagat ggcggaagga attcgcaagg gttcttccag
     6421 tctcgagatg ctggagaagc ttggttacga gcgaatgctc caagcagata agacaaagta
     6481 acgtgcagga cccggcgttt ggagattcca tgattggccg cgagtctgtc gatgcagagg
     6541 ttggaacggt cgagcggcag cagctgattc atctgagcca gattcagtgg ccgatcagtt
     6601 acgggcggaa tagatcgttt ggcggcgatc gaacaataca cttggacgcg aataaataac
     6661 gtcagacaag taatacggaa aggtttatgg cttcttcatt agatgaaaca gttatttccc
     6721 gtgcaattca cttcggcacg gcaacattac acgaggcaat gggcaggaag ggtgctttgc
     6781 cccatcgcat caaacccatt tcaccgcgca tgagggttgc gggactggcg gtaacggtca
     6841 gctccccacc gatggacaat ctcatgctgc atcaggcgat ctacgccgct gagcctggat
     6901 cgatcatcgt ggcggaggta aatcgcggct acgaaggcgg ctattggggc gagatcatga
     6961 cgattgcggc gcagcagcga aagatcgctg gtctggtgat cgacggttgc gtgcgtgatg
     7021 cggatttgat cgaacacctt ggcttcccgg tgttctcgcg tggcttatcg gtgcgcggca
     7081 cggacaagca gggcggcggg cacatcaact ggccgatcct actcggcgac atcaccatca
     7141 acccgagtga tctggtggtc ggtgaccgag acggcgtgat cgtcatcccg gcagcggaga
     7201 tcgccgcaac tctcgaagcg gcgcagaagc gcgaagccaa agaagatcag acaaagaggg
     7261 atctgatggc gggaaaatcg accttaggga tctacggatg gccgccgccg aagctgaatt
     7321 cctgagaccg cctcgctgac gaaacatacg aattacatcg cagtgaccgc ggcgtcccgc
     7381 attggtcgtg gcgggtagct gaacttgcgg caaaaccgat cgggacactc aacggggaag
     7441 catttctgca tagacggctg gctaactcca cggcggactg gaacgatggc gcttagcgag
     7501 ggtcgcgggt ccaacactga actgatctcg cgcaagcatg tcctggtttg ccgcccgagc
     7561 gttcagccgg tttaaatcaa tccctaaact ccgcactttg ctgcgcgaat aatccaccat
     7621 tccaagtggg tcggataccc cggccgccgc agatgtgatc tgaatccgca agaccatacc
     7681 gatttaatct ttcgagaatc ttgcctgaga ttcttatggc ttgtctgtgc tcgaaaatcg
     7741 cgtttcaatc aattagtgac tcgcaatccg aatcaactga tcgatttgac agctcgctgg
     7801 atggagcaca tgcgccgggg cgagttcgat gaggcttggc gcaacagcga catactgctg
     7861 cgacatcact tggctgcgcc acgcgacgct cacgatgtgc cacgtcatca acaggtcatt
     7921 tggaacggcg cttcgctgca gggtaagcga gtgctggtac ggtgctacca tggcctcggc
     7981 gacaccattc agttcatccg ctacattccg ctgttaaaag ccattgcccg acacgtaacc
     8041 gtttgggcgc agccctcgtt ggtaacgctg ctaagcacag cctgcggaat tgaccgcatg
     8101 tttccgctcc acgacggaac gccggattgc gactacgagg ccgacgttga agtgatggaa
     8161 ctgccgtacg tgtttcgcac gaccatcgaa accataccgg ctcaggttcc ttattttcat
     8221 ctggatgcgt ctgccctcga ggattgtagg atgaaagtag gtctcgtctg gagaggcggc
     8281 gattgggatc aacgccggga tgtgccgttc gatctgctca ctcaactcgc ggagcttcag
     8341 gagattagct tctacgcgct ccaacaagag acgagtgcat tcgaacacca ccaaaggtta
     8401 aaaatgattt tgccggcggg agccgatgct ctgactacgg cgcgcgtcat gctggcgctc
     8461 gatctggtca tctccatcga tagcatgccc gctcatctgg ccggtgcatt aggaatccgc
     8521 agctggacac tgcttcaaaa gaatgcggac tggcgctgga tgagtgatcg cacggatagc
     8581 ccctggtacc cgaccatgcg tctgtttcga caggagcagg cgggagattg ggagccggtc
     8641 gtggcgcaag ttaaacgcga gctgcgaaaa cttctctgat ctcgcaagtc aatttagctt
     8701 tcctctccct ccctgacctg ctggcgccac cccggcctag tctttagcta gaacaaagag
     8761 cagatgatat gcggcacctc gcgcgcttgc agatctctat ttcaagcaac cgacgagtgg
     8821 catcgattgc gacgaattca cagttggttt ttggattccg agcgggctcg gtctcttgcg
     8881 caaagtaatc accgcggata tacccgattt taattcatag aatcgactca cgagaattat
     8941 caaacggagg gagtgatgat tcccagcaaa caagccacat tgaccgatga atctacctac
     9001 cagcagtggg tgcgaaccga aggaattccg atcatccgag aatttttcat tcaagacatc
     9061 acgaaggttg ccctggaacc ttgggatcga atgggcgggt caggcgttta cttgaatctc
     9121 atcggcaccg gcgaagccaa tgacgcctat atcttggaga tttccccggg ccaaaaaggc
     9181 agagagcagc accatctatt tgaggaaatg atctatgtga tcggcggccg gggcgcgacc
     9241 tcgatttggc aagccaacgg caaaaaacaa acctttgaat ggcaaaccgg gagtttgttt
     9301 tcaccgccga tcaattgcca ctaccagcac ttcaacggca gcggttcgga accggcgcgg
     9361 atgctggcgg tgaccaacgc gcctgtgctg ctgaatctat ttcacaacgc cgattttgtc
     9421 ttcaacaaca gttatgtttt ttcagatcga ttctccggcg aggaggaata tttttccggt
     9481 aaaggcgagt cgttcccggg acgaatctgg gacacgaatt ttgttcccga cgtgcacacg
     9541 atggcgctcc agtcttggaa agagcgtggc gcagggggca aaaacatcat gctggagctt
     9601 tcggaaaata ctatgggagc gcacatttcg gaatttccag ttggcactta caagaaggcg
     9661 catcggcatg gtcccggcgc acatgtgatc atcatcgccg ggaagggcta ctcgtgcctg
     9721 tggccgcagg gtgaggagat caagcgcttc gattggcagc ccggctcgat cgtcgttccg
     9781 cccgagttct ggttccatca acatttcaat gcgggcaacg agccggcgcg ctatttagcg
     9841 ctgcgcgcca gcggcaaaaa gttcagaggc ctgacaaaac tctacggcgt ggatgaagac
     9901 gttaaaaaag gcggatcgca aatggagtac gaggacgagg atcctcagat ccacaaagaa
     9961 ttcgaacagg aactcgagaa agtcggcgcg cgctgcgcca tgggcggtca gcatccgcgc
    10021 tgcacggaga aagcagcggc ctagcaggct attaggattc tgctgctgcg atccgtaatt
    10081 ttattctgct gccttcaggg cgagtgatcg aacatcagca gcgcggatct aattacgtaa
    10141 cccaacctga caggccgccg cgttctcaga ggcggaaact gattgtctct tgcgaaacgt
    10201 ttgctgcgct catatctgcc gttcgactgc cgagccggtg atttttcaca ttcagatata
    10261 gcttacccgt cgttctgtcg gtcggcgact ttgtttccct gagcttcacg gccatcccgg
    10321 tcctcgcctt cattccatcg gaaacggatg atgcttggta tgcgccaggc gatcatcagg
    10381 gtaaccgccg agactaatag cccgcccact acagttgttt cacgggcgcc gatcagcgga
    10441 accatcagtc cggtttcagt ctgccccaac ggctgaaggc cgcggttgct caattgaaag
    10501 acccccatta ttcgcccgcg aaatctttcc ggggtggtta attgcagaat cgtgccgcgc
    10561 gccgccgacc agatcgcgtc ggtggcgccg accagg
//
LOCUS       DASUVS010000252         8241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_102668, whole genome shotgun sequence.
ACCESSION   DASUVS010000252 DASUVS010000000
VERSION     DASUVS010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8241
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102668"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1282)
                     /locus_tag="VF182_13920"
     CDS             complement(215..1282)
                     /locus_tag="VF182_13920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX6438231.1"
                     /translation="MPSSFVTTQEIITQARRNLSQHAWHYLTGGAESETTMCRNRLAL
                     DSLAFRPRVLVEVAKIDPSTTFFGNRLRIPVMLAPIGSLQIMTSEGAVAVARAAHEFG
                     TLNFVSSVTQPSLEETAAATPHPKIFQLYVQGDMRWVETLLTRVKNAGYRALCLTVDT
                     AYYGRRERQMMDRWLPPSRRLSGYEHRSAITWETMAAIKEFAGLPFILKGVATAEDAA
                     LAVERGVSAIYVSNHGGRQLDHNRGTLAMLPDIVEAVAGRAAVVLDGGIVRGSDVLKA
                     LALGADAVTIGKLQGWGLGAGGQQGLIRVLEILESEIITSMALLGVTRIDQLTPEYLC
                     KCQPIALSHEMSAFAHMPRLF"
     gene            complement(1399..1776)
                     /locus_tag="VF182_13925"
     CDS             complement(1399..1776)
                     /locus_tag="VF182_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006508628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX6438232.1"
                     /translation="MSFETGRMKGDLKVRTKEFALAVVRLYSGLPKRREAQIIGDQVL
                     RSGTSVGAHFREAQRAKSRPDFISKIEGGMQELEETVYWLELLEETGFATLKTLGTLK
                     SEADELMAIFVTIAKKTKAQLKK"
     gene            complement(1866..2735)
                     /locus_tag="VF182_13930"
     CDS             complement(1866..2735)
                     /locus_tag="VF182_13930"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6438233.1"
                     /translation="MEPPPLQIGNWRVVWRRFKQHRVAYVGFFFFVFLLLLVIAGPHL
                     LPYELDQVSLAERMHGPSLTHWFGTDELGRDTWVRIMHGGRVSLAVGLIVGLSTVLLG
                     GLIGILAGYVGGTLDNILMRMVDTIYTIPRIVLLLVLSKVAGPGLLNIIIILVALEWT
                     NAARLARSAVLSFREQEFVVAAHCLGASRWRVMLRHLLPNSLAPLIVAATLDAGAAIR
                     AETTLSFLGLGIQPPTPSWGNLLTNASTNIFIAPLQVFLPGIFIFFALMSFNLLGDAL
                     RDALDPRLSKRKG"
     gene            complement(2739..3710)
                     /locus_tag="VF182_13935"
     CDS             complement(2739..3710)
                     /locus_tag="VF182_13935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6438234.1"
                     /translation="MVLYIFRRLIQLVPLLFFVSALIFFLLYSMPGDPLYRMLEGIPN
                     LRPEDYERLRKLYGFDDPFYIQYWKWLWQLLQLNPGYSREYGQPVIDIIIPALKNTLV
                     LTITAVVLGKLIAIALGIFSAVRQYSIGDYLLTALTFVAYSVPAFWLGLMMIILFSVK
                     LGWLPTSGIVNPDLTPGTWEATVDWMKHLILPVSVLAISEIIQVQRFMRSSLLEVLRQ
                     DYLTTARAKGLSEQTVIGRHALKNALIPVVTVIAVTMPRVIGGSTVVETVFAYPGMGR
                     LLYTSVMGNDYVIAMTVVMILAVAVVFFNLLADILYGWLDPRIRYQN"
     gene            complement(3861..5588)
                     /locus_tag="VF182_13940"
     CDS             complement(3861..5588)
                     /locus_tag="VF182_13940"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6438235.1"
                     /translation="MSDGNKKSNSKTRVDLSRRQFIRLAGTTAAAFGTLPWPNAPLFA
                     AQPARKRIIIHGERQVTSLGYHNRRETEHVSMVDAGLVGQNPATLERVPVLAEELPSV
                     KKGTWKIDPQKKTMITVYKLRPNLKWHDGKPYTSKDFEFGWQIAKHPQFPMPDRLVPE
                     MISKIETPDDRTLVIHWNDLYNEAYAIQYTHVRAFPRHLLQDAFNAGDMKAFANLPYW
                     NKNFIGAGPYRVTEWDAGSRMELEAFNDFALGKPKIDRVTYKTVEDNNTNLAAVLAGE
                     VDLCMRSTISFDGAMILRDQWEKAGKGKVHISPASWTWLNLSRDNALFSDVRVRRALL
                     HAIDRDAMVQNMFRGEKVVSDIPLSRVRKIYKKALSAVTLYKYDPERAKKLLAEAGWK
                     PGSDGILTNDKGERMEFEFRVTAERRDHEQAEAIIADYWKKIGVRGNIKNLPNRLLNS
                     ADNRNRWPGAFIGSHNVTVEEWPERFHSKNIPTAENKYAPENVSGWNDPRKDAILDEI
                     NSIIPDARSEQLQLDFLKMFTEALPHLPLYYSPEILVVKRGLTGVTPRQESGGQNSSS
                     WNMQQWDKT"
     gene            5797..6504
                     /locus_tag="VF182_13945"
     CDS             5797..6504
                     /locus_tag="VF182_13945"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX6438236.1"
                     /translation="MAVDNFEDHCWKDVVTPEILKTYTPYHRETFIGQRPALLAIDLY
                     NLAYEGGAKEPHELVDTHKSSCGVYAHNAIKPTQELFALARSLKIPIFYTTTETRKEA
                     NPGKVYATNRQRVKIDPQAFEIREEFKPEPDDVVIFKERASGFFGTPLIAHLTQKGID
                     SLIVCGESTSGCVRASSVDGYSLGYHVSIVEECVFDRSPLSHKVNLFDMHHKYCDVMK
                     LDEVKEKIKQERPRTAK"
     gene            6631..7425
                     /locus_tag="VF182_13950"
     CDS             6631..7425
                     /locus_tag="VF182_13950"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6438237.1"
                     /translation="MYSTANGLKIYYEVSGEGFPFVMVHANPFDHNLWMYQIAHFSTY
                     FKVIAIDIRGYGRSDKPTTPFTLADMADDVLGVCRDESVNEAILGGVSVGSGMALLLG
                     LDHPELFKALILVGGSSGPGDAIDGRIHGYTKVGIEKYHIQHLKELVAPDFPETKLGK
                     YLLNMYVERDPWLSGESIAQIFRARGGTDLTPRLGSMKVPTLVINGEYDNSLKGGRRT
                     ASLIPGAVHKILPKTGHACCIEDPAGFDAFVIDFLRANRLLPSSPS"
     gene            7484..8038
                     /locus_tag="VF182_13955"
     CDS             7484..8038
                     /locus_tag="VF182_13955"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6438238.1"
                     /translation="MTINIERLKSMTPPPGMDFKIGKLGHIVLQVSDLERSTAFYTQV
                     LGLRVSDVYPETMMPGGMVFLRCNTDHHCLALVGAGERQAANQELHHFAFEVPTLAEV
                     MRARDRLRANQVQIDFEGRRRAGCQIAVEFRDPDNHSVEIYWGVDQVGSDGRARPQNE
                     WHGVKSLEEAIAHPVPGQDTHLPK"
     gene            8035..>8241
                     /locus_tag="VF182_13960"
     CDS             8035..>8241
                     /locus_tag="VF182_13960"
                     /inference="COORDINATES: protein motif:HMM:NF018349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1152 domain-containing protein"
                     /protein_id="HEX6438239.1"
                     /translation="MSSGSPSLEDIIGESSRSLVIGIGGGGDVAGALATARFLELCGL
                     EFVLGGLSWERRVFDPLPGPRMLSE"
BASE COUNT         1869 a         2320 c         2213 g         1839 t
ORIGIN      
        1 gtttccttca ccgacttgta aaaccggcac acgttggttt caacctgctc gttgacagcc
       61 aagttttcat cggccggttt gttccaagtg ccgtcgaaac aaagaactat tcttcgcatg
      121 atcatccctt ctactgcgca attaagccac taccgagcgc agccatttta aaatttccca
      181 atcccgcggc gcaccccatg ctgcaatagc gcgcttaaaa taatctcggc atgtgcgcaa
      241 acgcgctcat ctcatggctc agcgcgatag gctgacattt acataaatat tcgggcgtta
      301 gctgatcgat gcgggttacg ccgagaagcg ccatcgaggt gatgatctca ctttcaagaa
      361 tttccaatac ccttattagc ccttgctgcc ctccagctcc gaggccccac ccttgaagct
      421 tgccgatggt cacagcgtcg gcgccaagcg cgagcgcctt caacacatcg ctgccgcgca
      481 cgatgccgcc gtctaaaacc accgcggctc tgccggccac ggcttcgacg atgtccggca
      541 gcatcgcgag cgttccccgg ttgtgatcga gttgccggcc gccgtgattg gatacataga
      601 tcgcgctgac gccacgctcg acagccagtg cggcatcttc ggcggtcgcg acccccttga
      661 gaataaacgg taatccggcg aactctttga ttgccgccat ggtctcccaa gtgatcgccg
      721 agcggtgttc gtagcccgat aggcggcgac tcggcggcag ccagcgatcc atcatctgac
      781 gctcgcgcct gccgtaatag gcggtatcca cagtcaaaca gagagctcga taaccggcat
      841 tcttgacccg agtaagaaga gtctcgaccc acctcatatc gccctggaca taaagctgaa
      901 agatctttgg gtgcggcgtc gcggcggcgg tctcctcgag gcttggctgg gtaaccgagc
      961 tgacgaagtt aagtgtgccg aactcgtgcg cagctctggc gacagccacg gcgccttcag
     1021 acgtcatgat ctgcagcgaa ccgatcggcg ccagcatgac tggaattctg agcctgtttc
     1081 cgaaaaacgt tgtcgaggga tcgatcttcg ccacttcgac gagaacgcgc ggccgaaatg
     1141 cgagactatc taacgcgagc cgatttcggc acatggtggt ctccgactcg gcccctccgg
     1201 tcagataatg ccaggcatgc tggctaagat tgcgccgcgc ctgggtaatg atttcctgag
     1261 tcgtgacgaa gctggatggc atactggctc cagtaaacat gaattatgaa agcggaaagc
     1321 ggaaattgga acagactcgt aaagatattt atgagagctg atatctgcga ctttatccta
     1381 atgagaagtc gaatcgagct attttttcag ctgcgccttc gttttcttag cgattgtaac
     1441 aaaaatggcc atcaactcgt ccgcttcact tttcagtgtt cccagtgtct ttagtgtcgc
     1501 aaatccagtc tcttcaagca gctcgagcca atagactgtc tcttctaatt cctgcatgcc
     1561 accttcgatt ttgcttatga aatctggtct tgatttcgcg cgctgcgctt cccggaagtg
     1621 cgctccgaca gacgtgcctg atcttaatac ctggtcgccg atgatttgcg cttctcgtct
     1681 cttgggcaag ccagaataaa ggcgaacaac tgccagcgca aattccttcg tcctgacttt
     1741 caagtctcct ttcatccttc cagtttcgaa actcatccgt gttccttact cacgcgctgt
     1801 aatcgttcgc atccgcccat ttgaaggttg ttgatctccg ctcttcagct ttccgctttc
     1861 atcactcatc cttttctttt agataaccgc ggatccaggg cgtcccggag cgcgtcaccg
     1921 agcagattga agctcatcag ggcgaagaaa atgaagatgc ccggtagaaa gacttgcagc
     1981 ggggcgatga agatgttggt tgaggcattg gtgaggagat ttccccagct tggcgtcggc
     2041 ggctgaatcc cgagcccgag aaaactcaag gttgtttcgg cgcggatggc agcgccggca
     2101 tcaagggttg ctgcgacgat cagcggcgcc agcgagtttg gcagcaggtg gcgcagcatg
     2161 acgcgccagc gagacgcacc cagacaatgg gcggccacca cgaattcctg ctcgcgaaaa
     2221 gaaagcaccg cgctgcgcgc cagccgggcg gcgttagtcc attctaatgc gaccagaatg
     2281 atgatgatgt tcaaaagacc cggtccggct actttggaca gcaccagtaa tagtacgatg
     2341 cgcggaatcg tatagatggt atccaccatc cgcatgagaa tattgtccag tgttccgccg
     2401 acgtaaccag cgagaatgcc gatcagcccg cccaagagta ccgtgctcaa cccgacgatg
     2461 aggccgactg caagtgagac tcggccgccg tgcatgatcc tgacccaggt atcgcgcccc
     2521 agttcatccg tgccgaacca gtgcgtcaga gacggtccat gcatgcgctc tgcgagcgaa
     2581 acctggtcga gttcgtaggg gagcaggtga ggtccggcaa tcaccagaag cagcagaaaa
     2641 acgaagaaaa agaatcccac ataggcgacg cggtgctgtt taaagcgccg ccagacgacg
     2701 cgccagttcc ctatctgaag cgggggcggc tccatgggtc agttttgata gcgtatgcgt
     2761 ggatcgagcc agccgtacag aatgtcggcc agcaggttaa agaacaccac ggccacggcg
     2821 agaatcatca ccaccgtcat ggctatgaca taatcattgc ccatgaccga ggtgtagagg
     2881 agccggccca ttccggggta cgcaaaaacg gtttcgacca ccgtcgaacc gccgattacg
     2941 cgcggcatcg tcaccgcgat gacggtgact acgggaatca gcgcattctt cagagcgtgg
     3001 cggccgatca cggtctgctc gctcaatccc ttggcccggg cggtggtcaa gtagtcctgg
     3061 cgcagcacct ccagcaaact tgaacgcata aaacgctgca cctgaatgat ttcggaaatc
     3121 gccagtaccg acaccggcaa tatgagatgt ttcatccaat cgaccgtcgc ttcccaagtg
     3181 cccggcgtca agtcgggatt gacgatgccg gacgtgggca gccaaccaag tttgacggaa
     3241 aaaaggatga tcatcattag cccaagccag aacgccggaa ccgaataggc gacaaacgtc
     3301 agcgccgtca gcaagtaatc gccgatggaa tattgtcgaa cggcagaaaa aattcccaac
     3361 gcaattgcga tcagcttgcc gagaaccacc gccgtaatcg tcagcacgag agtatttttc
     3421 aacgccggga taatgatatc gatcaccggc tgcccgtact cgcgcgagta accggggttc
     3481 agttgaagca actgccagag ccatttccaa tactgaatgt agaaaggatc gtcgaagccg
     3541 tagagtttgc gcaaacgttc ataatcttcc ggacgcagat tgggaatgcc ttcgagcatg
     3601 cgatacagcg gatccccggg catggaatag agcaggaaaa aaatcagcgc ggagacgaag
     3661 aagagcaggg gaacgagctg gatcagacgg cggaagatat acaaaaccat aaaaaacttg
     3721 gttcaacagt tcaaggtgac tagggttaac aggcctgctt cgatagtcaa aattcaagtt
     3781 ccatcgcgac atttggaacc catctgtccc gtttatgctg acgttgaacg ttggaacgct
     3841 tgaactgttg aacgagcgtt tcaggtctta tcccactgct gcatgttcca actgctggag
     3901 ttttgcccgc cgctttcctg ccgcggcgtc actccggtga gccctcgttt caccaccagg
     3961 atttccggcg agtagtatag cggcagatgc ggcagcgcct cggtgaacat cttgagaaaa
     4021 tccaattgca gctgctcgga gcgcgcatcc ggaataatcg agttgatctc atccaaaatc
     4081 gcgtctttgc gcggatcatt ccaaccggag acgttttccg gagcatactt gttctcggcc
     4141 gtgggaatgt ttttgctgtg aaaccgttcg ggccattctt ccactgtaac gttgtgcgaa
     4201 ccgatgaacg cgccgggcca acgattgcgg ttatccgctg agttgagcag ccggttcggc
     4261 agatttttaa tattgccgcg cacgccgatc ttcttccagt aatcggcgat gatcgcctcg
     4321 gcctgctcgt gatcgcgccg ttcggccgtg acacggaact cgaactccat ccgctcgcct
     4381 ttatcattgg tcaaaatgcc gtcggagccg ggcttccatc ccgcctcggc aagaagtttt
     4441 tttgcccgtt ccgggtcgta cttgtagaga gtcaccgcgg ataaggcttt tttgtaaatc
     4501 ttgcgcacgc gcgagagcgg aatatcggaa accactttct ctcccctgaa catattctgc
     4561 accatcgcgt cgcgatcgat ggcatgcagc aacgcacggc gcactctgac gtcactgaac
     4621 aatgcgttgt ctcggctcag gttgagccag gtccaggagg cagggctgat atgcactttg
     4681 ccttttcccg ccttctccca ctgatcgcgc aggatcatcg cgccatcgaa agaaatagtg
     4741 ctgcgcatgc acaggtccac ctctccggcc agcaccgccg ccagattggt attgttgtcc
     4801 tcgacggttt tgtaagtcac tcggtcgatc ttaggcttgc cgagcgcgaa gtcgttgaaa
     4861 gcttcgagct ccatgcggct gccggcgtcc cattcggtta ctcggtatgg gccggcgccg
     4921 atgaagtttt tgttccaata aggcaaattg gcaaatgcct tcatatcgcc ggcgttgaag
     4981 gcgtcctgca acaggtggcg cggaaaggcg cgcacgtgag tgtactggat cgcataagcc
     5041 tcgttgtaga gatcgttcca atggatcacg agagtgcgat cgtcgggcgt ttcgattttt
     5101 gaaatcattt ccggcacgag tcgatccggc atgggaaatt gcggatgttt cgcaatctgc
     5161 cagccaaact cgaaatcttt ggaggtgtac ggcttgccgt cgtgccactt gagattagga
     5221 cggagcttat agaccgtaat catggttttt ttctgaggat cgatcttcca cgttcccttc
     5281 ttaaccgagg gcagttcctc ggccagcacc ggtacgcgtt cgagagtggc aggattttga
     5341 ccgaccaggc ccgcatccac catggacaca tgctcggtct cgcgccggtt gtgatagccg
     5401 agactcgtaa cctggcgctc gccatgaatg ataattcttt ttcgcgcggg ctgagcagcg
     5461 aacagtggcg cgttcggcca tggcagagtg ccaaaggctg ccgcggttgt gccggccaat
     5521 ctgatgaatt gacgccggct cagatcgacg cgagttttgc tattcgattt cttgttcccg
     5581 tctgacatca agtatcctcc ctttaatgcc gatggttgtc ggcatccata taactaacct
     5641 cgaacttgag tgtcaatcga tacgccgatc acaagatccg tcgccgcctc gagcgcgccc
     5701 acgggatttt agaatgcctt tgtctccggg gtcttccttg acaaggcttc aagccaggcg
     5761 atacaacaaa caatctaatc gagtaaggag aaacccatgg cagtggacaa ttttgaggat
     5821 cattgttgga aagacgtcgt aactccggaa attcttaaga cctacacgcc gtatcatcgg
     5881 gaaaccttta tcggccagag accggcgttg ctggccatcg acctttacaa tctcgcttat
     5941 gaaggcggcg cgaaggagcc tcatgaatta gtcgacaccc acaagagctc gtgtggcgtc
     6001 tatgcacata acgcgatcaa gccgacgcag gaacttttcg cgctcgctcg ttccctaaaa
     6061 attccgatct tctacaccac gacagaaacg cgcaaggaag ccaatcccgg taaggtttat
     6121 gccaccaatc ggcagcgggt gaagatcgat ccacaggcgt ttgaaattcg cgaagagttc
     6181 aagccggaac ccgatgacgt cgtgatcttc aaagagcgcg ccagcggctt ctttggcact
     6241 cctctgatcg ctcatttgac acagaaaggg atcgacagcc tgatcgtttg cggcgaaagc
     6301 actagcggct gtgtccgggc cagctcggtc gatggctatt cgctgggtta tcacgtgtcg
     6361 attgtggaag aatgcgtctt tgatcgcagc ccgctttcgc acaaggtcaa cttgttcgat
     6421 atgcatcaca agtactgcga tgtcatgaag ctcgatgaag taaaagaaaa gatcaaacag
     6481 gagcgcccgc gcaccgcgaa gtagaacgat tgtccaagcc gttcaattcg ttcaagccgt
     6541 ccaacgaact gtacagaaca cattctgacg atttgaacgg agcgcagcga ttgaacggct
     6601 tgaacatttg aaactaagca tttcccaaag atgtacagca ccgccaacgg tctaaaaatc
     6661 tactacgagg tttccggcga gggatttccc ttcgtcatgg tccatgccaa tccgttcgat
     6721 cataaccttt ggatgtatca gatcgcgcac ttctcgacct atttcaaagt gattgccatc
     6781 gatatccgcg gctacgggcg ttcggacaag ccaaccacac cctttacctt ggcggatatg
     6841 gccgatgatg ttctcggcgt ctgccgcgat gagagcgtca acgaagctat ccttggcggc
     6901 gtcagcgtcg gctcgggaat ggcgcttctg ctcggcttgg accatccgga attattcaag
     6961 gctctgattc tcgtcggcgg cagcagcggt ccgggcgacg ccatcgatgg acgaatccac
     7021 ggctatacca aagtcggcat cgagaaatac cacattcaac acctgaagga actggttgcg
     7081 ccggattttc ccgagaccaa actcggcaaa tatctgctca acatgtatgt cgagagggat
     7141 ccctggcttt ccggagagtc catcgcccaa atcttccgtg ctcgtggcgg taccgacttg
     7201 acgccgcgcc ttggttcaat gaaggtcccg accttggtga tcaacggcga atacgacaac
     7261 tctctcaaag gcggccggcg caccgcctcg ctgattccgg gcgcggtgca caagatcttg
     7321 cccaagactg gtcacgcctg ctgcatcgaa gatccagccg gcttcgatgc attcgtgatc
     7381 gatttcttgc gcgccaatcg tctcctgcct agttcgccga gttgaactag tccatcagtt
     7441 tctcatcgag ccggtcgctt tagcggcaga aagaggaacg accatgacca tcaatatcga
     7501 gagattgaag agcatgactc ctcccccagg aatggacttt aagatcggca agctcggcca
     7561 tatcgtgctg caggtcagcg acctggaaag gtccactgct ttttacaccc aagtgctcgg
     7621 cctcagagtc tccgacgttt accctgagac aatgatgccg ggcgggatgg ttttcctgcg
     7681 ttgcaacaca gaccatcatt gcctggccct ggtcggtgcc ggcgagcgac aagccgccaa
     7741 tcaagagctg catcacttcg cctttgaagt tccgacactc gcagaagtta tgcgggctcg
     7801 tgatcgcttg cgcgccaatc aagttcaaat cgatttcgaa ggacgccgcc gggctggctg
     7861 tcaaatcgcc gtggaattcc gcgatccgga caaccattcg gtggaaattt attggggcgt
     7921 ggatcaagtc ggcagcgatg gtcgggcccg gccgcagaac gaatggcacg gcgtgaaatc
     7981 gctcgaggaa gctatcgctc atccggtacc cggtcaggac actcaccttc ctaaatgagt
     8041 tccggctctc cttctctcga agatataata ggcgaatctt cgcgcagcct ggtaatcggc
     8101 atcggcggtg gcggcgatgt cgccggcgca ctggcaacgg ccagatttct cgaactttgc
     8161 ggtttggaat ttgtcctcgg cggcctctcc tgggaacgcc gggttttcga tccgctgccg
     8221 ggcccgcgca tgctgagcga g
//
LOCUS       DASUVS010000253         8307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1633571, whole genome shotgun sequence.
ACCESSION   DASUVS010000253 DASUVS010000000
VERSION     DASUVS010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8307
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633571"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VF182_13965"
     CDS             <1..798
                     /locus_tag="VF182_13965"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6438240.1"
                     /translation="GVTHPAVRHLTVTASAIATLSELSESRAFLGFGVGATGPGNIGM
                     KPVSIPELEENILILTKLFAGETVNLGGKDVRCLFPPGHVPIYIGTRATQVMKIACRL
                     ADGLIYTGETETLKAVVERVRGYTAQANRRHAEVRFVYRLPCSLSDDAAEARAKIKGI
                     IASVAFTHLGRLYNRNELHDQQDRRALERLRVHYDTYHHMGPEHQHLIRDEWVDRFGL
                     AGTPDQVRAKVREFIDAGIDEITIVPCIGDSKISLLKSFARDVMDKL"
     gene            complement(1273..2334)
                     /locus_tag="VF182_13970"
     CDS             complement(1273..2334)
                     /locus_tag="VF182_13970"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6438241.1"
                     /translation="MSIPIATLIFVLFAGAAFPAPAAERPSANSDERAVADFYRGKVV
                     RIVVGFPPGGGADVYSRIVARYLGHSIPGNPTVAVTNMPGAGSMVAGNHIFNAGATDG
                     TEIGMLNGAVILEQLFGNPAVQFDMAQFRYLAVPVTEIYVMIVARRSGITRFSELIGA
                     KAKQAVLGAIPNSTLEHAPILLRDALSANLKVVSGYKGSADIRLAIDSGEIGGFFNPW
                     STVKASSLEKFTSGQWSVILQLSDQSLPDLPSANVPTIADLTTEESQRMLLRYGTLAP
                     NQFGKVYMLRPGVPADRAAFLEAAFAKTFADLNFRAEAEKSQLEISPLYGGSIRKIVG
                     DFLTMPPAIKERLKRSLKR"
     gene            2861..3103
                     /locus_tag="VF182_13975"
     CDS             2861..3103
                     /locus_tag="VF182_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438242.1"
                     /translation="MIEFCGGARAATGSQHLIEVNGQRILLECGPYQDKHAEAYERNR
                     NFLFDAMVLSYAHINHSGKAANSLFCANLHLREQCV"
     gene            3433..4158
                     /locus_tag="VF182_13980"
     CDS             3433..4158
                     /locus_tag="VF182_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX6438243.1"
                     /translation="MVGGSTAFGTGLESDDETFAQRLEHLLDAEVINAAVVGHASGQE
                     LVSLVMELVDLQPDLVIALNGWNDYERPLTSKFRGSNGFAEIEEQLKTLQSVTDDSLL
                     TRVSNLYWVLFPNITKKLEPLWLRLFKREKQTGETDLGLAGAVYATNMTKMRRISEAF
                     HSTFLCVLQPDRDRQQRYRVFRETAKMHLQQAGIVALDLSELDEPGIKAERYMDEMHL
                     DAVGNQAIARIIANKIVDDDLLR"
     gene            complement(4230..5315)
                     /locus_tag="VF182_13985"
     CDS             complement(4230..5315)
                     /locus_tag="VF182_13985"
                     /inference="COORDINATES: protein motif:HMM:NF023327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HEX6438244.1"
                     /translation="MSLNQILFSSSNEDGFSELAALDPNARRIVLCITGSGSRVLNLL
                     TVNPVRISCIDINPAQTHLAELTFLAFKHLNSYEDLLRFIGIEAGKGRWRTYYALRPH
                     LSPPARQFWDQRCNMIQRGIIYSGKWERIFRITGKIFSILKHDARDQLFQASSLHEQT
                     LAWNRCDTWRWRMMLKLFSWALKWQCVFNRSGIHLVIDKMELDSHLNALLKHAASHHL
                     FRTNAFLYLILYGRYSSEGPLPFYLQRSQFTKIKNSSTQVDFHTGSLLSFLSTSSCPE
                     FDAFSLSNFSSYTTEDDYRRTWEKIVIKAKHGARFCERQFLVYRTLEQYVAGESLDRD
                     QEMETLLERTDSTLFYKFLIGTIHHKS"
     gene            complement(5569..6369)
                     /locus_tag="VF182_13990"
     CDS             complement(5569..6369)
                     /locus_tag="VF182_13990"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6438245.1"
                     /translation="MPFADSEGTQIYYDEKGKGEPVFLCLTGWCVHHTIFLPLAERLS
                     AQHRVLVIDWRAHGKSQASDQDFGYAEMLADVHSVIQASGARSVIPIAQAHGGWVAID
                     LRRQLGQRVPKMISISWNPIYITGDPLASPFLSARQALQDQASWRETLEQMLTTWLSG
                     APPSVDAHIRTEMKSHGFKDWARAGREISAMYAREGDPLRALSRLSPPVPVLHVYAEP
                     RTPEYLSAQESFARDHSWFVIRRLDSVSHFPTLEVPDDTARVIREFIQ"
     gene            6999..7241
                     /locus_tag="VF182_13995"
     CDS             6999..7241
                     /locus_tag="VF182_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438246.1"
                     /translation="MRTILFLAGLLLMASALIAGKLLFEHFPSAPNWALEASLAWWLA
                     VTGVNMWIAVSKVGYSVAEKLLILVLLFAVPPAAAV"
     gene            7275..7859
                     /locus_tag="VF182_14000"
     CDS             7275..7859
                     /locus_tag="VF182_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX6438247.1"
                     /translation="MGKNRLEAFSDGVLAIIITIMVLEMKVPHGENLKTLAPLIPVFM
                     SYVLSFVYLGIYWNNHHHMLHTCSRVTGGILWANLHLLFWLSLFPFVTGWMGENHFAA
                     LPTALYGGVLLAAALAYWILQQTIIAVQGPGSPLKTAIGNDWKGKLSPVLYVLGVVST
                     LQWPWVGQALYVAVALLWLVPDRRIERSLRSDGA"
     gene            complement(8097..>8307)
                     /locus_tag="VF182_14005"
     CDS             complement(8097..>8307)
                     /locus_tag="VF182_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438248.1"
                     /translation="PIIGFANNVRHEKTRNVRQLNIFIVFLTERDCAPVRIFHGHLYV
                     NLRAEQLWVNHEEPMRRGFGAGMGY"
BASE COUNT         2034 a         2191 c         2227 g         1855 t
ORIGIN      
        1 ggcgtgacgc atcctgccgt gcgccatctg acggtcacgg caagtgctat agcgacattg
       61 agcgagctaa gcgaaagccg ggcttttctt ggtttcggcg tcggagctac cggaccaggt
      121 aatatcggca tgaaaccggt atccatacca gagctcgaag aaaatattct tatcctgaca
      181 aagcttttcg ccggtgagac agtaaacctg ggagggaaag atgtccgctg tctttttccg
      241 ccaggacacg tgccgatcta catcggtacg cgagccacgc aagttatgaa aatagcctgc
      301 cggctcgcag atgggctgat ctacactgga gagactgaga cactgaaggc tgtcgtcgaa
      361 agggttcgcg gatatacggc gcaagcgaac cgacggcacg ccgaggttcg tttcgtttat
      421 cggctgccgt gttcattaag cgacgacgct gccgaggcaa gagcaaaaat caaaggtata
      481 atcgcgagcg tggcgtttac ccacctggga agattataca atcgcaacga gcttcacgac
      541 cagcaggatc ggcgcgccct ggaacgcctc agagtacatt acgacaccta ccatcatatg
      601 ggtccggagc atcaacatct gattcgcgac gagtgggtgg accgatttgg acttgccgga
      661 acgccagatc aggtgagagc gaaggtgcgt gagtttatcg atgccggcat cgatgagatc
      721 accatagttc cctgtatcgg agattcgaaa atatctctcc tcaaaagctt tgcccgcgac
      781 gtgatggata agctgtagaa tggctgacga aggcaatcct gggatgacgc gcggcgtcag
      841 gcatcacttc ttgacttatt cttgagccga tgctgcaact ccgactgatt gtatcccatc
      901 tctccacaga tgccgcatgc aaccggctga tcatcgacgg atcgcaattg cggtgttcgc
      961 caaatctttc gctttgatcc cgcaccactc ccgcgcgtca tagaccagca ataaaaattg
     1021 ccagggggcg ggagatcacc ccaaagcctt gaacctgatg ggtgactggt gcgaacctac
     1081 agcccgacac cgattgattg aaaacagagg cgcgtcaatt agaaattaca catccgctag
     1141 gattgaaaag agaataagaa aaaatctcac aggagcgact gtcctctcga cccgcctgct
     1201 aacgggtggg tgacgcggca acaagaagcg tgagcgtaag gagcgaactg tcttgccgtc
     1261 caggtgccct gttcaccgtt tgagagaacg cttcagccgt tccttgatgg ccggcggcat
     1321 agttagaaag tcgccgacga ttttccggat cgacccaccg tagagcggtg aaatttccaa
     1381 ttggctcttt tccgcctcgg ctcgaaaatt cagatcagcg aaagttttgg caaacgccgc
     1441 ttcgagaaac gcggcccgat ccgccggcac gcccgggcgc agcatataaa ccttgccgaa
     1501 ttggttcggc gccagagtgc cgtagcgcaa cagcatgcgc tggctttcct ctgtcgttag
     1561 atcggctatc gtcgggacat tagccgatgg cagatcgggc agcgattggt cgctcagctg
     1621 aaggatcacc gaccactggc cactggtgaa tttctccaag gagctcgcct tgaccgtgct
     1681 ccacgggttg aaaaaaccgc cgatctcgcc cgagtcgatg gccaggcgga tgtccgcgct
     1741 gcctttgtag ccggacacta cttttaaatt cgcactcaga gcatcgcgca gcaggatcgg
     1801 cgcatgctcc agggtcgaat tgggaatggc gccgaggacg gcctgtttcg ccttggcgcc
     1861 aatgagctcg ctgaacctcg tgatccccga gcggcgcgca acgatcatga cgtagatctc
     1921 ggttacgggt acggcgagat agcgaaattg cgccatgtcg aactggactg ccgggttgcc
     1981 gaatagctgt tcgagaatca ccgcgccgtt gagcatgccg atctctgtgc cgtcggtggc
     2041 cccggcattg aagatatgat tgccggcaac catgctgccg gcgcccggca tgtttgtcac
     2101 cgcgaccgtt ggattaccgg ggatcgagtg gccgagatag cgggcgacaa tccttgaata
     2161 gacgtcagcg ccgccgcccg gcggaaagcc gacgacgatg cgcacgactt tgccgcgata
     2221 gaaatccgcg acagcgcgct catcgctatt cgcgctcggg cgctcggccg ccggtgccgg
     2281 aaacgcggcg ccggcaaaca ggacaaaaat caacgtggct attggaatac tcaagcgttc
     2341 taccttgccg cgcggtaatc tagcacaacg tatgaagaat gcgggagcag acatgagttg
     2401 ttgatgaatt gagccaacgc tgcagctccg actcagatcg cttgtcccgg ttctccacat
     2461 atgccgcatc cagccggctg attcatgaac accgggaatg ggatcaatct ttacctatag
     2521 cacaagcgat atcctcgatc tcgattgata ccagttcact tcaatcgtgg caccgcagaa
     2581 caccccgggc cgctgattaa gagcgaatcc agggcggacg agaagcgaac gtaaccagag
     2641 agtccactta tttttgaggt ctgcttgctt attcttcgga aacggaacgt gtatgttctg
     2701 cccaggtctc acacaaaaca agcacagcag tgaatcaccc ctcgccttgc cggcagcctt
     2761 tgcggatttt aatatcgcga aaggctgatc tatgtattac tgatataccc agtgtagata
     2821 cggcgaagat catgttttta gaaccgctgg aggaagcgac atgatagaat tttgcggcgg
     2881 tgcgcgggcg gccacgggtt cgcagcacct gatcgaggtc aacgggcagc gtattctgct
     2941 cgaatgcggc ccataccagg acaagcacgc agaagcgtac gagcgcaacc gcaattttct
     3001 gttcgatgca atggtgcttt catatgcgca catcaaccat tcaggcaaag ccgccaacag
     3061 cctgttttgc gcgaacctcc accttcggga gcaatgcgta tgagacggtc ggggaaaata
     3121 ttaccgattc tgtttgtcaa cgcgttgatc ctttgtttcg ccacgttcgg gttggagttc
     3181 aaactggccc aggaccgtca tcaagcagag gcaaagtggt ccaagcaggt gaggccgtat
     3241 tattggccgt cgctccagac gcacgacggc atcgctctcg ggacgaggtc cggaccgttg
     3301 aaattagtcc tacatccgtt cctcagttac gccaacttac cgaaccaaaa aaatccgcac
     3361 tttacgatca atcacctcgg gtttagaggc ggcaatatcc cgaaggacca aaagactaaa
     3421 aagcgcatta ttgtggttgg aggctccacg gcatttggca ccgggctcga gtctgacgac
     3481 gaaacctttg cacaacgcct cgaacatctg ttggatgccg aagtgatcaa cgctgcggtc
     3541 gtcggtcatg cgtccggaca ggaactcgtg tctctggtaa tggaactggt ggacttacag
     3601 ccagatctgg tgatcgcgct gaatgggtgg aatgattatg agcgccctct gaccagcaag
     3661 ttccgcggat caaatggctt tgcggaaatc gaggagcagc tgaaaacgtt acaatctgtg
     3721 accgacgact cattgctcac gagggtatcg aacctgtatt gggttctgtt tcccaacatt
     3781 accaagaagc tggagccact gtggctcagg ctcttcaaga gggaaaagca aacaggtgaa
     3841 acggatcttg gcctggccgg tgcagtctac gcgaccaata tgaccaaaat gcgcaggatt
     3901 tcagaagcct ttcattccac atttctctgc gtcttgcagc ctgatcgaga taggcagcag
     3961 cggtaccggg tcttccgcga aacggcgaaa atgcacttac agcaggcagg gattgtcgcg
     4021 ctggatttaa gcgaactcga cgagccgggc atcaaagctg agcgatatat ggatgaaatg
     4081 catttggatg cagtcggcaa tcaggcaatc gcccggatca tcgcaaacaa gatcgtcgat
     4141 gacgacctac tccgctaagg tccacatgct gcggaaactt gcagattcca cccaaaaagt
     4201 tttagtcatc tcggcaatag gctgcacggc tatgatttat ggtggatcgt tccgatcaag
     4261 aatttgtaga acaaggtcga atcggtacgc tcaagtagcg tttccatttc ctgatcacga
     4321 tccagtgact caccggccac atattgctcc aatgttcgat aaaccaaaaa ttgtcgttca
     4381 caaaaccgcg cgccgtgttt ggctttgatc actatttttt cccaggtccg tctataatca
     4441 tcctctgtcg tgtacgaaga gaaattggat aaagaaaaag catcaaattc cgggcacgag
     4501 gaagtcgaga ggaatgacag caacgaaccg gtatggaagt caacctgcgt cgaactgttt
     4561 ttgattttgg tgaattgtga tcgctgcaaa taaaaaggta aaggtccttc agagctgtaa
     4621 cggccataga gaatcagata taagaatgcg ttggtacgaa aaagatggtg cgatgcggca
     4681 tgtttaagga gagcattaag atgagaatca agctccattt tatcgattac caaatggatt
     4741 cccgatctgt tgaaaacaca ctgccattta agcgcccaac taaagagttt aagcatcatt
     4801 ctccaacgcc aagtgtcgca ccggttccat gctaatgttt gttcatgaag gctgctggct
     4861 tggaacaact gatccctcgc atcgtgttta agaatgctaa atatcttccc ggtaatccga
     4921 aaaatacgtt cccatttgcc tgaataaatg atcccacgtt gaatcatatt acatctttgg
     4981 tcccaaaatt gacgcgccgg tggactgagg tgcggccgaa gtgcatagta cgtgcgccat
     5041 cggcctttac ctgcctcaat tccgataaag cgaagaagat cctcgtaact attaagatgc
     5101 ttaaatgcga gaaaggttaa ttcggcgaga tgggtctgag cgggattgat gtcaatgcaa
     5161 ctgattcgta caggatttac ggttagcagg ttcaacacgc gcgagccgct gccagtgata
     5221 catagaacaa tacggcgagc gttaggatct aacgcagcta actcggagaa tccgtcttca
     5281 tttgaagagg aaaacaggat ttggttcaaa ctcatgcact aagtgtcgat ggaggtctga
     5341 ctagtatgat aggtgcaaaa ctaaccagat caacctcctc taacgggtag ctaaacacgc
     5401 atgagcaata tggaatggct gcgaaagcgg gtagacacat cgcgaatctc gcaggattga
     5461 gccgttggag aaatcagttc aatgtacgct agcgtccagg ctgagccata atccgagcgt
     5521 aagtctagag ccagtggtca aacgtctgcg tggatctagg ccagtctcct attggataaa
     5581 ttctctaatt acacgagcgg tatcgtcagg tacttcgagt gtcggaaaat gacttaccga
     5641 atctaaccgg cgtataacga accaagaatg gtcccgagca aatgattctt gtgccgagag
     5701 gtactcaggc gtacggggtt ctgcatacac atggagcacc ggcacgggcg gactcaatcg
     5761 gcttaacgcc cgcagcggat caccttcacg tgcatacatg gcggaaatct cgcgcccggc
     5821 tcgtgcccaa tccttaaaac cgtgactctt catctcagtc cgaatatgcg cgtccacaga
     5881 tggaggcgca ccgctcaacc aggttgtaag catttgctcg agtgtctcgc gccagctggc
     5941 ctgatcctgc aaagcctgcc ttgcactcag aaacggcgaa gcaagcgggt ccccagtgat
     6001 gtaaatgggg ttccaactga tcgagatcat ctttggcaca cgctgaccta gctgccgacg
     6061 caaatcgatg gctacccatc caccatgcgc ttgagcgatg ggaattacgg accgcgcgcc
     6121 gctggcttga atcacggaat ggacatccgc gagcatctcg gcatagccaa agtcctggtc
     6181 ggatgcctgc gatttaccat gtgcccgcca gtctataacc aagacacgat gttgtgcact
     6241 caatcgctcc gccaggggca ggaaaattgt gtggtgtaca caccagccgg ttaagcacaa
     6301 aaataccggc tcgcccttgc ctttttcgtc gtagtaaatt tgtgttccct ctgaatcggc
     6361 gaacggcata ggtctggttc tcctgtctcg ctgccatgtt ttcgtcgcct agcgcgaagg
     6421 ttgacctgtt cggaggatcg ggcggcgaca aatgtcggtc ggcttatccc ggcgacgact
     6481 cgggcataaa aacgtcaaat cagctacaat gtccatcagc taaccgaggt ttgtcaacat
     6541 caaactctcc gcaaaagccg acaggcgtga cggcttatct ccttagtggt caacggccta
     6601 tttcgccaca ctatcgcaag gagacggcag gattgaacca atcgacgcta cagacaaatc
     6661 tcacaggatt ggtgcgatgg agaatcattc cgatcctggc gcagaacacc cctcggccgc
     6721 gatcaagagt caagtccaga gcgaccagaa ccgaacattg acaagaaagc ccaactattt
     6781 cgaagcccgc gtgctttttc ttcgacaacc gttaggtgta tatttccgcc cgcgttcagg
     6841 cacagccaag cacagcccga ggactcaagg tccttatgga tcggccctca accttgcagg
     6901 cggctagtgc cgatattaag atccgaaaga caagtccgta tagtcttagt taagcgccag
     6961 aattcaggtc tatttcaacg cggcaaagga tcgaccacgt gcgaacaatc ttgtttcttg
     7021 cgggtttgct gctgatggct tcagctctca tagcgggaaa actgcttttc gagcacttcc
     7081 cgtccgcgcc taattgggcg ctagaggctt cgctggcttg gtggctggcg gttactggcg
     7141 tgaacatgtg gattgctgtg tccaaagtcg gctactcggt cgccgagaag ctgctgatac
     7201 tggtgctgct atttgcagtg cccccggcgg cggcggtgtg agctggcgat tctttaggcg
     7261 agacgggacc gaacgtgggc aagaacaggc tcgaggcgtt tagcgacggg gttctggcga
     7321 tcatcatcac gatcatggtg ctcgagatga aggtgccgca cggcgagaac ctgaaaacgc
     7381 tcgcgccgct tatcccggtg ttcatgagct atgtgttgag cttcgtgtac cttggcatct
     7441 actggaacaa tcatcaccat atgcttcata cgtgcagccg ggtcacggga ggcattctct
     7501 gggccaacct gcatctgctt ttctggctct cgctgtttcc gtttgtcact ggctggatgg
     7561 gcgagaatca cttcgccgca ttacctacgg cactctacgg cggggtactg ctggcggcgg
     7621 cccttgccta ctggatactt cagcagacga ttattgcagt gcagggaccg gggtcaccgc
     7681 tgaaaacggc aattggtaac gactggaaag gcaaactttc gcctgtgctg tacgtgttgg
     7741 gagtcgtttc cacgctccaa tggccatggg tcgggcaggc cctgtatgta gctgtcgcac
     7801 ttctttggtt ggtgcctgac cgcagaatcg aaaggtcact acgctccgat ggcgcctaac
     7861 agccgcatgc agccgacgcg caagaacccg cgcgcagctg atgcgagatt cgccttaagg
     7921 agaactccgc gataacgttc ttggaatgct cgagccgatc aaggtaggcg atccggtggc
     7981 tttcgtcgcg aaaaatacct cggtgatttc cccgcacgat cacttggtat aaagcacctc
     8041 ccaatcgact ctcggattgc gctttacctg cgcgtatctc caggcaatcc gataaatcaa
     8101 taacccatgc cggcaccaaa tcctcgccgc attggttcct catgattgac ccaaagttgt
     8161 tccgctcgca gattgacata caaatgccca tgaaatatgc gaacaggagc acaatcacgc
     8221 tcagtgagga agacaatgaa aatatttagt tgcctgacgt ttctcgtctt ttcatgtctt
     8281 acgttgtttg caaatccaat gatcggt
//
LOCUS       DASUVS010000254        23208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_322668, whole genome shotgun sequence.
ACCESSION   DASUVS010000254 DASUVS010000000
VERSION     DASUVS010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 23208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23208
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322668"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VF182_14010"
     CDS             <1..357
                     /locus_tag="VF182_14010"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX6438249.1"
                     /translation="LIYSHFRSALSQVPTLEKILPVALSEAKETEQLTEYLYEPGAEE
                     LLSSLLPKITEIKIQRALLEATASEHGARMTAMDSATTNASKMMGSLTLQMNRARQAS
                     ITRELMEIVGTAEALK"
     gene            383..1792
                     /gene="atpD"
                     /locus_tag="VF182_14015"
     CDS             383..1792
                     /gene="atpD"
                     /locus_tag="VF182_14015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX6438250.1"
                     /translation="MSMGRITQVLGAVIDVEFSNGSLPSIYNALKVSNPAIDQEQWNL
                     VLEVAQHLGENTVRCIAMDTTEGLVRGMDVMDTGTGITVPVGEETLGRILNVIGEPVD
                     DNGPVNAKKSYPIHRPTPEFVDQETKVQALETGIKVVDLLAPYARGGKIGLFGGAGVG
                     KTVILMELINNVAMKHGGFSVFGGVGERTREGNDLWLEMKESGVINKTSLVYGQMNEP
                     PGARARVALTALTLAEYFRDDEGRDVLLFLDNIFRFTQANAEVSTLLGRMPSAVGYQP
                     TLATDLGELQERITTTHKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRQIAE
                     LGIYPAVDPLDSTSRILDPHVVGDEHYEVARQVQVILQRYKDLQDIIAILGMDELSED
                     DKLTVARARKIQRFLSQPFHVAEAFTGTPGEYVELKDTIRGFKEIVEGKHDDIPEQAF
                     YMVGTIEKAVEKAKKMAAQ"
     gene            1860..2276
                     /locus_tag="VF182_14020"
     CDS             1860..2276
                     /locus_tag="VF182_14020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX6438251.1"
                     /translation="MADKIRLKVVTPGRLLLDEEVDEVTAPGVKGEFGVLPNHISFLT
                     LLEPGEMSYKQGGIRRFLALSGGYAEVLDNVMTILAPAAEFGVEIDVNRARAAKDKAE
                     KRLSETQYEDKSFATAQEALRKSLVRLQVAVKEARK"
     gene            2300..3433
                     /locus_tag="VF182_14025"
     CDS             2300..3433
                     /locus_tag="VF182_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6438252.1"
                     /translation="MAKAATKEPVITMWSKEFTYDRWMQSQGIPIHRGYYIEDLRKVE
                     LEWWEERGCRAAFIQLNGQEGVTASRVSEVPPGKSLPPVKFALDEIVYVLAGRGLTTV
                     WAGEGKPKKSFEWQEHSMFLVPRNHWHVFSNMQGDKPIRLFHYSYMPIAMSALPEPDM
                     YFNNPYEPKVSVGDGDFYSEAKLVQAKDADEGLGMRAFWYGNFFPDMRAWNKLDANER
                     RGAGGKSVFMQFPNSELTAHMSVFAPQTYKKAHRHGPGRAIIIPAGEGYSIMWEEGKE
                     KIVCPWHECSMVTPPDKWFHQHFNAGGDMARYLALHPPMQFHGHAEKIEDRAKDQIEY
                     PNEDPFIRRKFEDELAKRGIKSIMPEECYTNPDYEWSKAMGKQ"
     gene            3535..5052
                     /locus_tag="VF182_14030"
     CDS             3535..5052
                     /locus_tag="VF182_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEX6438253.1"
                     /translation="MHDDIYDHDHPDNFVIKEDEIVTDIEGLEMFSLKSVGIDIGSST
                     SHLIFSQITLRREGASLSGRFKVTNREVLYRSPIMLTPYVSATKIDTDKVNAFIHSAY
                     KEAGLTPEDVDTGAVIITGEALKKENAQPIVEAFAKYSGKFICAAAGHNHEALLAAYG
                     CGAVDLSKAEHKTVLNVDMGGGTTKFSLVEDGVVSQTASINVGARLIAFDEKDIVTRI
                     EDAGRIMMKEIGHTVALGEKITEQQKEDFGAYMAKILFEVIERGPTSTMAKQLMVTPS
                     FVNYRGFKQIHHIVFSGGVSEHVYDRDSRAYGDIGPVLGSHMREYLKKLPKEVLREPV
                     EGIRATVIGAGEYTIQASGNTSYVSNLEALPVHGLKVIQAAMRKGVSVETALRQALAK
                     FDLPRFTSGLALSLTIDGQPDYQSVRKLAEGIAEVLRDSEDNECPLYLTLDVDIAKSL
                     GGILRDELKIHRDIIAVDGIEVGDLDYIDIGECLGITEVIPVTVKSLMFPTANSD"
     gene            5418..6554
                     /locus_tag="VF182_14035"
     CDS             5418..6554
                     /locus_tag="VF182_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6438254.1"
                     /translation="MSKAAHADRKMWSKIYTYDEWMEAQGIPIYRGYYIEDLRTLELG
                     WWAARECEAAFIQLVGQEGVTSARLTEIPPGKTVSPLKFSLDEIVYVVEGRGLTTIWA
                     GENRPKKTFEWQKHSMFLLPHNYTHQFSNMQGDKPARLLHYNYMPLTLSGVRDVNFIF
                     GNSYEGPDIQNQLDFYSEAKMVQQSLLDDSWGRRAYWYGNFFPDMRAWDKLDSNARRG
                     AGGRSVYIQFPDSEMSCHMSVFDARLYKKAHRHGPGRVIVIPVGEGYSIMWEEGKEKV
                     VVPWHEASCFVPPNKWFHQHFNAGGEPARYLALHPPMQFHGHAEKVEDRAKDQIEYVN
                     EDKWVREKFEAELAKRGITSLMPPEAYVNPEFEWNWSPFLRKSS"
     gene            complement(6575..7477)
                     /locus_tag="VF182_14040"
     CDS             complement(6575..7477)
                     /locus_tag="VF182_14040"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6438255.1"
                     /translation="MGKIGLAVVSPAPLIRPNYVVNFAKNCEAMGVDSIWTIDRVAYD
                     NLEPLTMLAAAAGATQRIRLGTSVLLGNLRHPAHLAKIVATLDFISNGRVTLGLGFGS
                     RESDYKAVEVPFDKRGSRAAEQVNLIKRLWTEENVTHTGKFFNIENVTIGPRPIQKPI
                     PIWTGGSAEVALKRAGTWADGYVCGSSAIPEFPQTWEKVAGHARAAGRNPDDIKKAAL
                     TFMAIDEDHGRAVKTIENYMTRYYGKVRGDVASTSLVGSPGAIADRIGSFLSRGLDTL
                     IIGVADPDPTQLDLFGDRILPRLQ"
     gene            7599..8033
                     /locus_tag="VF182_14045"
     CDS             7599..8033
                     /locus_tag="VF182_14045"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6438256.1"
                     /translation="MEASTFKLKTPHITGGRSHTPLAQTEHMTIGLNYYIPGRKNKLH
                     THPGEDHTFVVMDGQATFYNKDHQPTILNKGEGIILPENHYYYFQSTGDRPLALFRVS
                     AKKGTKPKVLRVDSDGNKRTDEEIDFVVVDGETVEGKFWELS"
     gene            8040..9191
                     /locus_tag="VF182_14050"
     CDS             8040..9191
                     /locus_tag="VF182_14050"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX6438257.1"
                     /translation="MKITEVKTLRLRAAIPQEGQVFSRSGVRNYRSTTLVQIDTDEGV
                     SGLGSCSGNGELIEFIVAKILSPLIVGMDPTRIDEVWDRAYIRGGHKEFGTRGIGVVA
                     MSGIDVALWDIMGKIHGQPLYQILGGQCRDKVPVYATALYPEEPATVAKRARGFADQG
                     FQGVKIKVGFDLDQDIEIVRAVRQELGRDFIVMTDANQGYTVDVGLKAAEAFADIGVF
                     WLEEPLFADDIAGHAQLRETTEVPIAVGENLHMCSAFENFVMRQAVDFLQPDVARAGG
                     ITEIRKIAALAAEHRVPISFHTWGDAVALAASIHLSAAMPECTVMELDYTYNPLRAEL
                     LREPLKVENGFMAPPDKPGLGIELDADALARFAFSGVEELAVRQKALIA"
     gene            9802..11178
                     /gene="mnmE"
                     /locus_tag="VF182_14055"
     CDS             9802..11178
                     /gene="mnmE"
                     /locus_tag="VF182_14055"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX6438258.1"
                     /translation="MYQEDTIAAIATAMGEGGVAIVRISGPNAEKTVRAIFVRSRQND
                     IKLRSHMLYQGSIRDPHTDLILDQVLVTIMRQPHSYTGEDVAEIHCHGGAFLVQRILG
                     VVLAQGVRHAERGEFTKRAFLNGRMDLSQAEAVLDLIRARTDQGIQLAVQNAEGVLSK
                     WVGDLREELIDILVQVEAAIDFPEEDIELSDRAALIAKIAALREKIGSIISTYRWGRL
                     FKEGARVCICGRPNVGKSSLLNALVGDNRVIVSAIPGTTRDVIEESLNLDGLPVVLWD
                     TAGIRETEDEVEKAGVGLSLEHIANADVVIAVLDGSESLTQEDISLLLNIDHSKSLVA
                     VNKSDLQQQLDLIEARHMFTSSCFVPVCAKDGTGIDGLKSKLRQFLLGKSIESPVILN
                     NLRHKNSLLRGQDVLATAIMHLERSDPPDIVAISLQEARESFEEVVGMVTSEDVLERI
                     FSSFCIGK"
     gene            11231..13129
                     /gene="mnmG"
                     /locus_tag="VF182_14060"
     CDS             11231..13129
                     /gene="mnmG"
                     /locus_tag="VF182_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX6438259.1"
                     /translation="MKNDQIFDVIVVGAGHAGIEACLAASRMGSKTLMLTLNLDHIGQ
                     MSCNPAIGGIGKGHLVKEIDALGGEMGRAIDETGIQFRVLNTKKGPAVRASRAQADKA
                     LYRMRMKRVLEACDSLTLRQGSVEKLLVRNGDIEGVETLIGEKFFGRTVILTTGTFLK
                     GLIHIGSQQYSAGRAGDFAVQGLSQYLAEYGFKVGRLKTGTCPRLDARTINFPRLQVQ
                     LGDSEPVPFSFSNRSIIQKQVPCYITYTNKRTHDLIRSAIHLSPIYSGVIQSRGPRYC
                     PSIEDKVVRFTDKIRHQIFLEPEGLDTVEIYPNGISTSLPLEVQVDLVHSIEGLEEAA
                     IMRPGYAIEYDYVEPTQLKPSLETKLIRGLYHAGQINGTTGYEEAAAQGLMAGINAAL
                     SNRGEDPLILGRDQAYIGVLIDDLVTKGTNAEPYRMFTSRAEYRLMLREDNADLRLTD
                     IGYKLGSVSDIAYEKLKQKMRDMAALAELLNRIQVTPCGETNSILAHFGSAPIINKIS
                     LIQLLRRPELTITDLIRFAPEIGSYSPHAATQLEVQVKYAGYLNRQTEMINRSKNMEG
                     SRLPQDIDYDAINGLSREVREKLEHIRPLSIGQAARIPGITPAAISLLSVYLKKHFGR
                     HELKISSS"
     gene            13242..14036
                     /locus_tag="VF182_14065"
     CDS             13242..14036
                     /locus_tag="VF182_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6438260.1"
                     /translation="MGKVIAIANQKGGVGKTTTSINLAASLSIEGKRTLLVDVDPQGN
                     ATTGLGLNRSELTSSLYDVITGTRVLAEVICGTELPLLFVVPASKDLVGAEIELVDVD
                     RREYRLKYALAPLTDEYDFIVLDCPPSLGLLTLNSLTAANSLLIPIQSEYYALEGLGA
                     LLETLQLVKQGLNPTLELQGIIITMFDGRNRLSHQVVEEIKKHFPEKLFKSIIRRNVR
                     LSESPSYGKPVCLYDPSSSGSQDYLDLAKELIAQGDTHVITENRIG"
     gene            14008..14892
                     /locus_tag="VF182_14070"
     CDS             14008..14892
                     /locus_tag="VF182_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX6438261.1"
                     /translation="MSLQKTGLGKGLSALIPVQPSESVRSTISDVPTTEVNIDKINPS
                     PFQPRRAFDETKLQELAMSIRNQGIIQPLVVRPKGERFELIAGERRWRAAMKAGLNSV
                     PVVVREASDHDALQLALIENLQREDLNPIEEATGYRRLQEEFIWSQEVVAEKVGKSRP
                     AVANALRLLSLPSEVQQEVAAGNLPAGQARALLGLQSEAVIITAYREVIARALSTRET
                     EKLVRNLKLGRRRRRESPAVDPDLRSLVEDLQRALGTKIRVLPKARSNKGKIEIEYYS
                     LPDLERIIGTMTTRSSLM"
     gene            complement(14928..16988)
                     /locus_tag="VF182_14075"
     CDS             complement(14928..16988)
                     /locus_tag="VF182_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX6438262.1"
                     /translation="MDAKHTNRLIHETSPYLLQHAHNPVDWYPWSDEAFAKAKREDKP
                     VLLSIGYSACHWCHVMERESFENEHIAQLMNDLFINIKVDREERPDLDEIYMNAVQML
                     TGRGGWPMTMFLTPDRQPFYGGTYFPPEDRHGMPGFPRILHGVAQAYRDKPEDVQKSV
                     EQILSALSRMSQSEDHEHAFSPETIGKAAAQIATAYDAENGGLGQAPKFPNPGVYELF
                     LRQYHQSGTLHFLEMVTHTLTRMALGGIYDHLGGGFHRYSVDGKWLVPHFEKMLYDNA
                     QLVRILALLYRVTVNEFFKRVMEDTLDYLVREMLHSEGGFYSTQDADSEGEEGRFFVW
                     SRTEVNQILSEEDGEIFCRIYDVTESGNFEGKNILHPILTVSQAAGLFKKTPDQIETV
                     IVQAKQKLFAARETRHKPFRDEKIVTSWNGLMLSGLAEAIKITTKPNFLAAASGTIQL
                     IFTKLISHGLLLHTYKDGTAKQLGFLDDYAFLAVGLLDFYEATLDRSALERAIDLTNV
                     MVREFWDSSGGGFFYTGKSHEQLVSRSKPVFDASVPSGNAKATELLLRLYHLTGNEDF
                     LSKAEIVLRSYSHAMESQPFGFAHMLCALNFYLRKPNDIVLVGKLEDSRTKELLAEIN
                     SIYLPNMTLQLVGPDQSLDEVSPLLAGKTQIAGNATAYVCRNQTCSAPVTQRDQLRSL
                     LVTQ"
     gene            complement(17159..17362)
                     /locus_tag="VF182_14080"
     CDS             complement(17159..17362)
                     /locus_tag="VF182_14080"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX6438263.1"
                     /translation="MTGTIKKIIKPKGFGFITPDDGSEEVFFHRGSMAPRNQIEDFNE
                     GDTVQFQTRKGEKGPVAFDLKIR"
     gene            17479..18555
                     /locus_tag="VF182_14085"
     CDS             17479..18555
                     /locus_tag="VF182_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438264.1"
                     /translation="MSSVLYYITGHGYGHAVRSCQVIWKLKERCPELTVHIRSTVPEW
                     LFEHPHYSVKQSSQALDVGIVQDDSLKMELGPTLEACKRLTARIPSLVEQELDFITKQ
                     NITLIMGDVPPLCFEVAARAGVPSVAITNFSWSGIYRGFLQALPDFLPLIEEMEDFYR
                     RTTLALTLPYSCAVEIFPMRERIPFITRSSALAQREARERFGIPSLATVVLLSFGGHG
                     LRRLHLDRLLRQKEFFFIGTGDELMKESNLLILPDAQRSYVDLVRAADVVVSKPGYGI
                     VSDIITHQVPLLYTDRGEFAEYPYLVKALEDCATADFIPQEDLISAHLTPYLKRLLTK
                     AKHWPAVSLNGAAVASEKVLSLLG"
     gene            18624..19562
                     /gene="ispH"
                     /locus_tag="VF182_14090"
     CDS             18624..19562
                     /gene="ispH"
                     /locus_tag="VF182_14090"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008747791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX6438265.1"
                     /translation="MAVEKVVLAKPRGFCAGVEMALATVERALEKHGPPLYVFHEIVH
                     NQHVVRDFIARGVHFIQSMDEVPAGARVIFSAHGISPEVWQQAGQKRLAFVIDATCPL
                     VDKVHREVKKFASQGYGIILVGHENHDEIVGTSGEAPEHVRVVANINQAKNVTVPDPE
                     RVAVLTQTTLSVDDTREIIDVLRRRFPRMVTPAKEDICYATQNRQDAVKQLTRAVDLV
                     LVIGSENSENSNQLCAVARSQGTPAYLISEIALLDPNWLQGVRRVGITSGASVPERLV
                     NEAVDYFIRQGAEVSHLGFIEENIHFALPTEVVHPQ"
     gene            19826..21187
                     /locus_tag="VF182_14095"
     CDS             19826..21187
                     /locus_tag="VF182_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX6438266.1"
                     /translation="MHHTKAIILILFFASTIPLTAALSQEVKPSGGAPDTAITAPTAA
                     PEPPVDKGDTAWILTSSALVLMMTIPGLFLFYGGLVRAKNVLGTIMQSFVVVALISIQ
                     WVLWGYSLAFGPDVGGWIGSLAWVGLDSVGATPNPAYAATIPHQAFMVFQMMFAIITP
                     ALITGAIAERTKFGTFIVFVLLWATFVYDPLAHWVWGKGWMGAMGALDFAGGTVVHIS
                     SGFSALAAAYFLGRRVGYGYETLTPHNLPFTVIGASLLWVGWFGFNAGSALAADGLAT
                     IAFVTTNTAAAAAALAWMFTEWFTHGKPTALGAASGAVAGLVSITPAAGFVGPLSAII
                     IGAVGGMLCYKACNLKAALGYDDSLDVVGVHGIGGTWGALATGLFASKAINPAGNDGL
                     FYGNPAQFGVQAVTVVATIAFALLMTFIILAILKATMGLRVSEEEERMGLDLTQHNER
                     GYA"
     gene            21275..22690
                     /gene="glnA"
                     /locus_tag="VF182_14100"
     CDS             21275..22690
                     /gene="glnA"
                     /locus_tag="VF182_14100"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX6438267.1"
                     /translation="MPVKDALEMAKKNKSEIVDFKFIDLPGLWQHFSIPATELSADIF
                     EQGLGFDGSSIRGFQSINESDMILLPDSDTAMMDPFREHSTISFVCNVLDAITREPYS
                     RDPRFVTQKAEKYLKSSGIADISYWGPEIEFFIFDSIRFDQNHHEGYYHIDSAEGFWN
                     SGNNDGPNLGNKIRYKEGYFPMPPMDQYQDLRSEMVLNLQKCGITVEVHHHEVATGGQ
                     TEIDMRYDTMTKMADNVLLYKYVVKNTARRRGKVVTVMPKPLFQDNGSGMHCHQSLWK
                     NGKNLFYDKKGYGLISDTARYYIGGLIKHAHALCAFIAPTTNSYRRLVPGYEAPINLV
                     YSARNRSACVRIPMYSDSEKAKRLEFRTPDPSCNPYFAFPAMLMAGLDGIRNKIEPPK
                     PIDKNLYELEPEEAAKVKSMPGSLDQALDALEKDHDFLLKGDVFTKDVIDTWLEFKRK
                     NEVDAIRLRPHPWEFALYFDI"
     gene            22759..23100
                     /locus_tag="VF182_14105"
     CDS             22759..23100
                     /locus_tag="VF182_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013571272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX6438268.1"
                     /translation="MKKIEAIIKPFKLDEVKQRLTAAGITGMTITEVKGFGRQKGHTE
                     LYRGAEYTVDFLPKVKIEILAPEEMAPKIVEAILESAQTGKIGDGKIFVSSVEEVIRI
                     RTSERGEEAIS"
BASE COUNT         5714 a         5583 c         6247 g         5664 t
ORIGIN      
        1 ttgatctaca gccatttccg ttccgccttg tcacaagtgc cgaccctgga gaagatcttg
       61 ccggtggctt tgagcgaagc caaagaaacg gaacagttaa ccgagtacct ttacgagccg
      121 ggggctgagg agctcttatc aagccttttg ccgaagatta cggaaataaa aattcagcgg
      181 gcgctattgg aggcgactgc gagcgagcat ggcgcgcgca tgacagcgat ggattccgca
      241 accacgaatg cttcgaagat gatgggaagc ttaacgctgc agatgaaccg agcgcgacaa
      301 gcttcgatca cccgtgagct gatggagatc gttggtacgg cggaagcgtt gaagtgagca
      361 acgaacggag gaagcgttag ttatgagcat gggaaggatc acccaagttc tgggagcggt
      421 tatcgacgtc gagttttcca atggttcgct gccatcgatt tacaatgcct tgaaggtcag
      481 taatccggcc atcgaccagg agcaatggaa cctcgtgctc gaggtggcgc aacatctagg
      541 cgaaaacacc gttcggtgta ttgctatgga taccaccgag ggtttggtgc ggggcatgga
      601 tgtcatggac actgggacgg gcattacggt gcctgtcggt gaggaaaccc tgggacgaat
      661 tttaaatgtt atcggcgagc cggtggatga caacggtccg gtgaatgcaa agaaatccta
      721 tcccattcac cggccaactc cggaattcgt cgatcaggaa accaaagttc aagctcttga
      781 aaccggcatc aaagtcgttg acttgctggc tccgtacgct cgcggcggta agatcggtct
      841 gtttggcggt gccggtgtcg gaaagaccgt catcctgatg gagctcatca acaacgttgc
      901 catgaagcat ggtgggtttt ccgttttcgg tggagtcggc gagcgcaccc gtgagggtaa
      961 tgacctgtgg ctggagatga aggagtccgg ggttatcaac aaaacctcgc tggtttatgg
     1021 gcagatgaac gagcctcccg gagctcgcgc tcgggtggcg ttgaccgcat tgacattggc
     1081 tgagtatttc cgcgatgacg aaggtcgtga tgtcctgttg ttcctcgata atattttccg
     1141 ctttactcaa gccaacgccg aagtttcaac ccttttaggg cgcatgcctt cggccgtggg
     1201 ttatcaaccg actctcgcga ccgatttggg ggaacttcag gaacgtatca cgacaactca
     1261 caagggatcg atcacctcgg tccaggcgat ctatgtgccc gcagatgact taaccgaccc
     1321 ggcgccggcc acgacctttg cccatttgga tgcaaccacc gtgctctccc gccagattgc
     1381 cgaactgggg atttatccgg cggttgatcc gcttgattct acgtcgcgaa tactcgatcc
     1441 gcatgtcgtc ggtgacgaac attatgaagt tgcgcgccaa gtgcaggtta tcttgcagcg
     1501 ttacaaggat ttacaagata tcattgcgat cttgggcatg gacgagcttt ccgaagacga
     1561 taagctgacc gttgctcgcg cgcgtaaaat acaaagattt ttatcgcaac cgttccacgt
     1621 ggctgaagca tttacgggta cgcccggtga gtacgtcgag ttgaaggata ctattcgcgg
     1681 gttcaaggaa attgttgaag gcaaacacga tgatatcccc gagcaggcgt tttatatggt
     1741 cggcaccatc gaaaaagcag tggaaaaagc gaagaaaatg gccgcgcaat aacagtccgc
     1801 ggttaattaa ttagaagata tctagcgtta attgatcttg actgtattct ccacggatca
     1861 tggcagacaa aattagactc aaagtagtga cgcccggccg gctccttttg gatgaggagg
     1921 tggacgaggt cactgcgccg ggagtgaagg gcgaatttgg cgtgttgccg aatcacattt
     1981 ctttccttac ccttctcgaa cccggtgaga tgagctataa acaaggaggg atcaggcgat
     2041 ttctcgctct tagcggcgga tatgccgaag tgctagataa tgttatgacc atcctagcgc
     2101 cggcggctga atttggtgtc gagattgatg tcaatcgggc tcgtgccgcg aaagacaaag
     2161 ctgaaaagcg gttaagcgaa acgcaatacg aggacaagag ttttgctacc gcccaagaag
     2221 ctctgcggaa atctctagtc cgactgcagg tcgccgtcaa agaagcgaga aaatagcagg
     2281 acaagaagga gtatatttta tggctaaagc cgctaccaaa gagcccgtca tcacgatgtg
     2341 gagcaaggaa ttcacctacg atcggtggat gcaatcccag ggaattccca ttcaccgggg
     2401 ctactatatc gaagatttgc gaaaggttga gttggaatgg tgggaggagc gtggttgccg
     2461 ggcggcattt atccagctca atggtcaaga aggtgttact gcatcccgtg tgtcagaggt
     2521 tcctccggga aagtcgctgc cgccagtcaa attcgcttta gatgagattg tttacgtatt
     2581 ggcaggtcgc ggcttaacga ccgtttgggc cggcgagggc aaacccaaga aaagtttcga
     2641 atggcaggag cattcaatgt tcctggtacc gcgcaatcac tggcatgtgt tcagcaatat
     2701 gcaaggtgac aaaccaatcc ggctttttca ctatagctat atgccgatcg ccatgtctgc
     2761 gctgcctgaa cctgacatgt acttcaacaa tccgtacgag ccgaaggtta gcgtcggcga
     2821 tggggatttt tattctgaag caaagttggt tcaggccaaa gatgccgacg aagggctcgg
     2881 gatgcgggca ttttggtacg gcaatttctt ccccgatatg cgtgcctgga ataagctcga
     2941 cgccaatgag cggcgcggcg ccggcgggaa gagtgtcttc atgcaatttc ccaattcgga
     3001 attaaccgcg cacatgtcgg tgttcgcgcc gcaaacctat aagaaagcgc accgccatgg
     3061 tcccgggcgg gcgatcatta ttcctgctgg cgaaggttat tcgattatgt gggaagaggg
     3121 caaagaaaag atcgtttgtc cgtggcacga atgcagtatg gtgacaccgc ctgacaaatg
     3181 gttccatcag catttcaatg ccggtggcga catggcgagg tatctagcgc ttcatccacc
     3241 catgcagttc cacggtcatg cggaaaagat cgaagaccgc gccaaggatc aaattgagta
     3301 tccgaatgaa gatccgttta tccggcgcaa attcgaggac gagctcgcca agcgaggtat
     3361 caaaagcatt atgccggagg agtgttacac caatccggac tacgagtggt ccaaagcgat
     3421 ggggaagcag taagaactct aacgctgtca ggccctgagg taagaatccc cgccgcgtgc
     3481 ggggattcgt gtttctatca agaacagaga gagattaagt tttaaggcgg aacaatgcac
     3541 gacgacatat acgatcatga tcatccggat aatttcgtca tcaaagaaga tgaaatcgtc
     3601 accgatatcg aagggctcga gatgttttcg cttaaaagcg tgggcatcga tatcgggtct
     3661 tcgacttccc atctaatctt ttcacagatc acactgcgcc gtgagggcgc atcactgtcc
     3721 ggcagattca aggtgaccaa tcgggaagtt ctttatcgct cgccgatcat gctcacgcct
     3781 tatgtgtcgg cgaccaaaat cgatacggat aaggtaaatg cctttattca cagcgcgtac
     3841 aaagaagccg gcctaacgcc ggaagatgtc gacaccggcg cggtgatcat caccggcgag
     3901 gcccttaaaa aggaaaatgc tcaacccatt gtcgaagctt ttgccaaata ttccggtaag
     3961 tttatttgcg ccgcagctgg tcacaatcac gaggcgctgc tagccgctta cggttgcggc
     4021 gcggtggact tatcaaaagc cgaacacaag accgtgctaa acgttgatat gggcggcggc
     4081 acgaccaagt tttcgttggt tgaagacggt gtggtttcgc aaacagcgtc catcaacgtc
     4141 ggtgcgcgac tcatcgcctt cgacgagaag gatatcgtca ctcggatcga ggacgctggc
     4201 cggataatga tgaaagagat cggtcacacg gtagcgctgg gcgagaaaat caccgagcag
     4261 cagaaggagg actttggcgc atatatggcc aagattcttt ttgaagtgat cgagcgagga
     4321 ccgacatcga ccatggctaa acaactcatg gttacaccgt cctttgtgaa ttatcgaggg
     4381 ttcaagcaga tccaccatat tgtattctcc gggggtgttt cggagcacgt ctatgaccgt
     4441 gattccagag cttacgggga tatcggtccg gtactcggca gccacatgcg tgagtacctg
     4501 aaaaagctgc cgaaagaagt gttgcgtgag ccggtcgaag ggattcgcgc gaccgtaatc
     4561 ggcgcaggcg aatacactat tcaagccagt ggcaacacca gttatgtttc caatctcgag
     4621 gccttacccg tgcatgggtt gaaagtaatt caagcggcaa tgcgcaaagg tgtctctgtc
     4681 gaaacggcac tcaggcaggc gctggcgaag ttcgacttgc cgcgttttac ttcgggtctg
     4741 gcattgtctc tcactatcga tggacaaccg gactatcaat cggtgcgcaa gttagccgag
     4801 ggcatcgccg aggttttgcg ggattccgaa gacaatgagt gtccacttta tctgacgctt
     4861 gatgtagata tcgccaaatc gttaggcgga attttacgag acgaactgaa gattcatcgc
     4921 gacatcattg cagtggatgg tatcgaggtt ggcgatttgg actatatcga tatcggtgag
     4981 tgcttaggaa tcactgaggt gattccggtc acggtcaagt cgttgatgtt tccaaccgcc
     5041 aacagcgatt agatcgggcc ttcgcgtctg atctgtcccg cagcacttaa atccgaacgc
     5101 catccgacaa attgggtgat cgcacagaag ccgcagttaa actattcgat ggacgagctt
     5161 ctgctctctg gtgctttccc ggtctcgatc cgcaaacatc ttaagctgag agatcgcttg
     5221 ccttcgtagg cgcgctgttt ccggccatgt acttcggctt cagcacccca ggcgccatga
     5281 tcaaacaaat ctgacttgga ggtcgccctt tcaaagtgtc gcggtagatt gctgtggtca
     5341 gtccagtcgg tcgtgcattt gtttcgattt ttaatggtga tatgactcaa tgaagttacc
     5401 tgcggggaaa ggataccatg tctaaagcag cgcatgcaga tcggaaaatg tggagtaaga
     5461 tttataccta cgacgagtgg atggaagcac aaggtattcc catttaccgc ggttattata
     5521 tcgaggattt gcgcactttg gagttggggt ggtgggcagc gcgggagtgc gaagcggcgt
     5581 ttatccagct ggtcggacag gaaggcgtca cttctgctcg attgaccgaa attccgcccg
     5641 gcaagaccgt atcgccactt aaattcagcc tggacgaaat cgtctacgtg gttgaaggtc
     5701 gtggtctgac aactatctgg gccggtgaaa atcggccaaa gaaaactttc gaatggcaaa
     5761 agcacagcat gttcttgttg ccgcacaatt acacgcatca gttcagcaat atgcaggggg
     5821 acaagccagc gcgcttgctc cactacaatt acatgccact gacgctatcc ggggtccgcg
     5881 atgtcaactt catattcggc aattcttacg aagggccgga tattcagaac caactggatt
     5941 tctattccga ggccaagatg gtgcagcaaa gtcttctcga tgactcatgg ggacgccgcg
     6001 cctactggta cggtaatttt ttccccgata tgcgcgcttg ggacaagctc gatagcaacg
     6061 cccggcgcgg cgccggcggc cgaagtgtct acatccagtt ccccgactct gagatgtcct
     6121 gccatatgtc agtctttgac gcgcgcctat acaaaaaagc ccaccggcac ggcccgggcc
     6181 gggttatcgt gattccggtg ggcgagggtt attcaattat gtgggaggaa ggaaaagaga
     6241 aagtggttgt gccctggcat gaggcgagtt gcttcgtgcc gcccaacaaa tggtttcatc
     6301 agcacttcaa tgccggcggc gagccggcgc gatatcttgc cctgcatccc ccgatgcagt
     6361 ttcatggcca cgcggaaaag gttgaagacc gcgccaaaga tcagatcgag tacgtcaatg
     6421 aagacaaatg ggttcgcgag aagtttgaag cagagctggc caagcgcggc attacatcct
     6481 tgatgccgcc ggaggcatac gtcaatccgg aattcgagtg gaactggtcc ccttttctgc
     6541 gtaaatccag ctaatcgaga aattgaatcg ctcgctactg gagtctcggc aggattctat
     6601 ccccaaacaa gtccaattgt gtcgggtcag gatcggctac gccaatgatc agtgtgtcga
     6661 gtccacgaga taaaaaagaa ccgatgcggt ccgcgatggc gcccggagag cctactaagg
     6721 atgtgcttgc cacgtctccg cggaccttgc cgtagtaacg ggtcatgtag ttctcgatgg
     6781 tttttaccgc cctgccgtgg tcctcatcaa ttgccatgaa ggtgagcgcc gcttttttga
     6841 tatcatctgg attgcgtcct gccgcgcgcg cgtgaccagc tactttctcc caggtctggg
     6901 gaaattccgg aattgccgaa ctaccgcaaa catagccgtc cgcccacgtg ccggcgcgtt
     6961 tgagtgcgac ctcagcgctg ccaccggtcc aaataggaat tggtttctga attggccttg
     7021 gacctatggt gacattctca atattgaaaa acttaccagt gtgcgtgacg ttctcttccg
     7081 tccagagacg tttgatgaga ttgacttgtt ccgctgctct actgccgcgc ttatcgaaag
     7141 gtacttctac ggctttgtaa tcgctttccc ggctgccgaa accgaggccc agtgtcacgc
     7201 ggccgttgga aatgaaatcc aaggtggcaa cgatcttggc gagatgggct gggtgtcgca
     7261 ggttgcccag tagcactgaa gtaccgagac gaattctttg tgtcgcgccg gctgcggcag
     7321 ctaacatggt caacggctcg agattatcat aggcgactcg atcgatagtc catatcgaat
     7381 cgacacccat ggcctcacag ttcttagcaa aattgacaac atagtttggc ctgatcaacg
     7441 gcgctggact gacaaccgca agaccgatct tacccatttg tttctccttt tttacggatc
     7501 caaaaacact gctaacgtcc ggctaaatta gcctgggcag agcgcccttt tcaagttgaa
     7561 ttctctgcga tatacgagtc acaaggagga cgcataaaat ggaagcttct acattcaagc
     7621 ttaagactcc gcacattact ggaggccgta gtcatacgcc gctggcgcaa acagagcata
     7681 tgacaattgg cctgaactac tacataccgg gacggaaaaa caaactacat acgcatccag
     7741 gagaggatca tacttttgtc gtgatggacg gacaagccac cttctataac aaggatcatc
     7801 agccgactat tctcaataag ggcgaaggaa ttattttgcc ggagaatcat tactattatt
     7861 ttcaaagtac cggcgatcgg cctctcgcct tgttccgggt gagcgcgaag aagggcacca
     7921 aaccgaaggt tttgcgggtc gattctgacg gaaataagcg caccgacgaa gagatcgact
     7981 ttgtcgttgt tgatggcgag actgtagagg gtaaattttg ggagcttagc tgagcacaaa
     8041 tgaaaatcac cgaggttaaa acacttcgtt tgcgtgcggc tatcccgcaa gagggacaag
     8101 ttttcagtcg ttccggagtg agaaactatc gttcaaccac tctggttcag attgacaccg
     8161 acgaaggggt ttccggattg gggtcctgct cgggcaatgg tgagctgatt gaattcatcg
     8221 tcgcgaagat tttgagtcca ctgatagttg gtatggaccc gacccgtatc gatgaagtgt
     8281 gggatcgggc ttatatcagg ggcggccaca aagaattcgg cactcgtggc atcggggtgg
     8341 tcgcgatgag cggaatcgac gtagctctgt gggatattat gggcaagatt catggtcagc
     8401 cgctctatca aatccttggc ggtcagtgcc gtgacaaggt tcctgtatat gctacggctc
     8461 tttatcctga agagccagca acagttgcta aaagagcccg cgggtttgct gaccagggct
     8521 ttcagggcgt gaaaatcaag gtcggtttcg acctggatca agacatcgaa attgttcgtg
     8581 cagtacgtca agagttgggc agagacttca ttgtgatgac tgatgcgaat caaggctaca
     8641 cggtagatgt cggattgaaa gcggccgaag cgttcgccga cattggggtc ttctggcttg
     8701 aggaaccgct atttgcggat gacatcgccg gacatgcgca gttgcgcgaa acgactgagg
     8761 tcccaattgc cgtcggagag aacttgcaca tgtgctcagc ctttgaaaac ttcgtgatga
     8821 gacaagccgt cgatttcctt cagccggatg tggcgcgcgc tggcgggatt acagagataa
     8881 gaaaaattgc ggctctcgct gcggaacatc gggtgccgat atcgtttcat acttggggag
     8941 acgcagtggc cctggccgct agcattcatc tttcagcggc catgccggag tgcaccgtga
     9001 tggagcttga ctatacgtac aatcctctac gggcggagct attacgagag ccgttaaaag
     9061 tggaaaacgg cttcatggct cctcctgata aaccgggatt gggaatcgag cttgatgccg
     9121 atgcgctcgc gcgtttcgcg ttctctggcg tcgaagagct tgcggtacgg cagaaagcgt
     9181 tgattgcata aattcggcag ttgttgcact cgcgggagcg tgagggtgcg atccgctgca
     9241 gcggggaagg cggcgtgtta gttgccatga ggttgcattc acaatcagga attccgcctg
     9301 atctgaccct ccgctgagat gtgtgctttt gaagtagccg tgtgtgagtg gtggcttcct
     9361 gtcctgcata aatcgttaag tctgctataa ctccccgctc attaacagaa cgggcttctg
     9421 caaggacttc cgtgcaccgg gctgtaagga tcctctactt ccgtagaggg tacggcgcag
     9481 acgctctgct agattaggtt tggtcccatc gatacaactc agatgactgg taattatagg
     9541 gggtagctta aatgtttcgt ccgaaggtat ggcttggaag ccgagaggcc tgatgactta
     9601 ggcacgtgag cttcagtgat tttatcattt cctgggggtc gctctacctg agaagcctta
     9661 tcgattttac gaacatagcc aatggtctag acaaaattag cgatttcgcg gaatcggaaa
     9721 gtcactgccc attagcctgt tttgataatc ggccgttttg ggttgccttg ccaatcgaag
     9781 ttgctacgat tgttaggttt tatgtaccaa gaagatacga ttgcggcaat tgcgacggca
     9841 atgggagaag gcggagtggc gattgtccgc atcagcggac cgaacgctga aaaaactgtc
     9901 cgtgcgatat ttgtgcgttc acgtcagaat gacatcaagc tgcgttccca catgctctac
     9961 caaggctcga ttcgcgatcc gcacaccgat ctaatcctcg atcaggttct cgtaaccata
    10021 atgcggcagc cccatagtta tacaggcgaa gacgtagccg aaatccattg tcacggcggc
    10081 gcctttttgg ttcaacggat tctgggagtg gtgctcgcgc aaggggtgcg tcatgccgaa
    10141 cgcggtgagt ttaccaagcg agcattttta aatgggagaa tggatctttc ccaggctgaa
    10201 gctgtgcttg atctgattcg tgcgcgcacg gatcagggca ttcaattagc agttcaaaac
    10261 gccgaaggtg ttctatcaaa atgggtgggc gatcttaggg aagaattgat tgatattctc
    10321 gtccaagttg aagcagcgat cgacttcccg gaggaagaca tcgaactttc ggaccgtgct
    10381 gcgttgattg caaagatcgc tgctctaagg gaaaagatag ggagtattat aagtacttat
    10441 cgatggggca gattattcaa agaaggtgca cgagtctgta tttgcggtcg ccctaatgtt
    10501 ggtaagtcga gtcttctgaa tgcgctcgtg ggcgataacc gtgtcatcgt cagcgcgatt
    10561 cccggcacca ccagagatgt tattgaagag tctctcaacc tggacggctt acctgttgtt
    10621 ttatgggata ccgcagggat tcgcgagacc gaggatgaag tggaaaaagc cggtgtaggc
    10681 ttgtctctgg aacacattgc aaacgcagat gttgttatcg cagtcctgga cggttctgag
    10741 tcgctgaccc aagaggacat ctcgctattg ttaaacatcg atcactcaaa gagtctggtc
    10801 gccgtaaaca agagtgatct gcaacagcaa ctcgatctta ttgaagctag gcatatgttt
    10861 accagctcat gctttgtccc cgtttgcgct aaggacggta ctggaataga cgggttgaag
    10921 tctaagctcc ggcagtttct gctcggaaaa agtatcgaat cgccagtgat cttgaacaac
    10981 ctgcgccata aaaattcgct acttcgcggc caagacgttc tcgcaacagc tattatgcat
    11041 ctcgagcgaa gcgatccacc cgacatcgtc gcaatcagtc tccaagaggc gagagaaagt
    11101 ttcgaagagg tagttgggat ggtcacaagc gaagatgttc tagaacgcat ttttagtagt
    11161 ttttgtattg ggaaatagat atttaacact ttttaaaatt atccgctggg agcatgtttc
    11221 acgtgaaact atgaaaaatg atcaaatctt cgatgttatc gtggttgggg ccggccatgc
    11281 aggtatagaa gcgtgtctgg ctgcctctag aatgggctca aaaactttaa tgctgacatt
    11341 gaacctcgat cacatcggcc agatgtcatg caatccagca atcggcggaa tcggcaaagg
    11401 acaccttgta aaagagatcg atgctttagg tggtgaaatg ggccgggcta tcgatgaaac
    11461 cggcatccag tttcgagtgt tgaataccaa gaagggtccc gcggtccgtg cctctcgagc
    11521 tcaggccgac aaagctcttt atcgaatgcg tatgaagcga gttcttgaag cttgcgacag
    11581 cttgactctt cgccagggaa gtgtcgaaaa acttcttgtt agaaacggcg atatcgaagg
    11641 agttgagact ttaatcggcg aaaaattttt cggcagaacc gttatcctaa ccaccgggac
    11701 tttcctcaaa ggactcatcc atattggtag tcagcagtac tctgccgggc gtgccggcga
    11761 ttttgccgtg caaggattga gtcaatatct ggccgagtat ggcttcaaag tagggagact
    11821 caagacagga acctgccccc gtttagatgc gcgtacgatc aactttcctc gcttgcaggt
    11881 tcaactgggc gattccgagc cggtcccatt ttcgttctcg aatcggtcga taatccagaa
    11941 gcaggtccct tgttacatta catacaccaa taagcgaact cacgacctga ttaggtctgc
    12001 gattcacctg tcaccgatat actcaggtgt gattcaaagc cgtgggccgc gctattgccc
    12061 atccattgag gataaggtag tgcgatttac agataagatc cggcatcaga tatttctcga
    12121 acccgagggg ttggatacag tagagattta cccgaatggc atttccacta gcttgccact
    12181 ggaagtacaa gtcgatctag tacattcgat tgaaggtctg gaagaagccg cgattatgag
    12241 acccggttat gcgattgaat atgactatgt cgaaccaacc cagctaaagc cttcgttgga
    12301 aactaagctt atccgggggc tataccacgc cggccagata aacggaacca caggttacga
    12361 ggaggcagcg gcgcaaggac ttatggctgg aatcaacgct gccttgagca atcgggggga
    12421 agatcctcta atcttaggtc gcgatcaggc gtacattggg gttttgatag acgacttggt
    12481 aaccaaggga actaacgctg agccgtacag gatgtttact tcccgagcgg agtatagact
    12541 gatgttgcgc gaagataatg cggatctcag actgacagat atcgggtaca agctcggttc
    12601 agttagcgac atagcttacg agaagttgaa gcaaaaaatg cgcgatatgg ccgctcttgc
    12661 agaattgctg aaccggatcc aggtcacacc gtgcggtgaa acaaattcaa tattggcgca
    12721 cttcggctcc gcaccgatta ttaataagat ctctctcatt cagttacttc gtaggccgga
    12781 gctaacaatc accgatctga tccggttcgc tccggaaatc ggaagctatt cgccccatgc
    12841 cgcaactcag ctagaggtgc aggtgaaata tgcgggatac ctcaatcgcc agactgaaat
    12901 gattaaccgt tctaagaaca tggagggatc tcggctccct caagacattg attacgatgc
    12961 tattaacgga ctatcacggg aagttcggga gaagttggaa cacatccgcc cgttatccat
    13021 cggccaagcg gctagaattc cgggtatcac tccagcggcg atttcattac tctctgtgta
    13081 tttgaagaaa catttcggcc gccatgaact gaagatttct tcctcctaaa tatttaacgt
    13141 ttcacgtgaa acattaattt tctgctttcc ttccgacctg ctttgtgtta caactttcgc
    13201 caacacatac acgtaagtac ctaatttata taggaatcag cttgggaaaa gtcatcgcga
    13261 ttgctaacca aaaaggtggt gtgggaaaaa ctaccacttc gatcaatttg gccgcttcat
    13321 tgtcgatcga agggaaaaga acattgctgg ttgatgtaga tccccaggga aacgctacaa
    13381 ccggcttagg tttgaatcgt tctgagctaa catcgagtct ttacgacgtt attactggaa
    13441 cccgtgtgct cgcagaagtg atttgtggaa ccgaactgcc tctgttgttt gtcgtccccg
    13501 catccaaaga cttggtaggt gccgagatcg agttggttga cgtcgacaga cgtgaatatc
    13561 gccttaaata tgcgttggct cctttgactg acgagtatga ctttattgtt cttgattgcc
    13621 caccatcctt aggcctcctg accctgaaca gcttgactgc agcgaattcc ctgttgattc
    13681 cgatacaaag cgaatactat gctctcgaag gactgggtgc cctcctcgaa accttgcaat
    13741 tggtaaaaca agggctaaat cctactttgg aattgcaggg cattatcata acgatgtttg
    13801 acggtcgtaa tcggctctcg catcaagtcg ttgaagagat caaaaaacat tttcccgaaa
    13861 aattatttaa gtcgatcatc cgcagaaacg tgcgtctgag tgaaagcccg agttatggca
    13921 agccggtttg tttatatgat cctagctcta gcggttctca agattatctg gatttggcta
    13981 aagaattaat cgcacaggga gatacgcatg tcattacaga aaacaggatt gggtaaaggt
    14041 cttagcgcgc taattcctgt acaaccgtcg gagtctgttc ggagcactat aagcgacgtt
    14101 cccacgaccg aggtcaacat tgacaaaata aatccaagtc cattccagcc gcgccgggcg
    14161 ttcgatgaaa ccaagcttca agagttagcg atgtcgatcc gtaatcaagg gatcattcaa
    14221 ccattggttg ttcgaccgaa gggtgagcga ttcgaactga tagctggcga acgccgctgg
    14281 cgcgccgcaa tgaaggcagg actgaatagt gtgccggtcg tcgtgcgaga ggcaagcgac
    14341 catgatgcgc ttcagcttgc tttgatcgaa aaccttcagc gtgaggactt aaatccaatc
    14401 gaagaagcca caggttaccg ccgccttcag gaagagttta tctggagcca ggaagtagtg
    14461 gcggaaaaag ttggcaagag ccggcctgcc gttgcgaatg ccttgcgact tttatcgctt
    14521 ccctctgaag tgcagcaaga agtggccgct ggcaacctgc ccgcaggaca agcgagagcc
    14581 ttgctgggat tgcagagcga agcagttatc atcactgcct atcgtgaagt catcgccaga
    14641 gctctttcca cacgcgagac tgaaaaactt gtgcgcaact tgaagcttgg cagacgacgc
    14701 cgacgagagt cgccggctgt agatccggat ctccgttctc tggttgaaga cttacagcgg
    14761 gcgctcggaa ctaagataag agtgttgccg aaggcgcgaa gcaataaggg taaaatcgaa
    14821 atagagtact acagccttcc cgacttggag cgaatcatcg gtactatgac aacccgctcc
    14881 agcctgatgt aatgcacggg ggttgaccga caatgatcaa acaaggatca ctgagtaact
    14941 aacagagatc tcaattgatc gcgctgagtc accggcgctg agcacgtttg gttccggcaa
    15001 acgtaagcag ttgcgttacc agcgatctga gttttgcctg caagcagagg tgaaacctcg
    15061 tctagagatt gatcaggtcc aacaagttgc agggtcatgt tcgggagata aattgaattt
    15121 atctccgcga gcaactcttt agttctcgag tcttctagtt tgcctacaag cacaatgtcg
    15181 ttcggttttc gcaggtaaaa attcaaagcg cataacatgt gggcaaaacc aaagggctga
    15241 ctctccattg cgtgtgagta cgagcgtagg actatttcag ctttgctgag aaaatcctcg
    15301 tttcccgtta aatgataaag ccgcagcaac aactcggtcg ctttggcatt gccggaagga
    15361 accgacgcat cgaaaaccgg tttactgcgg cttaccagtt gttcatgaga cttccccgta
    15421 tagaaaaacc ctcctccgga tgaatcccaa aattcccgta ccatcacgtt ggtaagatca
    15481 atggctcttt ctaatgcaga acggtctaga gtcgcctcgt aaaaatccag tagcccgacc
    15541 gctagaaaag cataatcatc taaaaaccct aattgtttcg ccgtcccatc cttatacgta
    15601 tgaagtagca agccatggga aatcaatttc gtgaaaatga gttggatagt gccgctggcg
    15661 gccgccaaga aatttggctt cgttgtaatc ttgatcgctt cagccaaccc tgacagcatc
    15721 aggccgttcc atgaggtaac gattttctcg tcgcgaaatg gtttgtgtcg ggtctcacgc
    15781 gctgcgaaaa gcttctgctt tgcctgaact attacggttt caatctgatc cggagttttt
    15841 ttgaacaagc ccgccgcctg ggacacagtc aggatcggat gaagaatgtt tttgccctcg
    15901 aaattaccgg attccgtgac gtcgtaaatg cggcagaaaa tttcgccgtc ttcttcgctg
    15961 agaatctgat tgacttcggt ccgactccag acgaaaaacc tgccttcctc accttcgctg
    16021 tccgcatctt gtgtcgagta gaatccccct tcactgtgca gcatctcgcg aactaaataa
    16081 tctaacgtgt cttccatcac tcgcttgaag aactcgttga cggtaactcg gtagagtagc
    16141 gccaagattc gaacaagctg tgcgttatcg taaagcatct tctcgaaatg agggacgagc
    16201 catttcccgt caactgaata acggtgaaat ccaccgccta ggtgatcgta aattccacct
    16261 agggccatcc tagtaagggt gtgtgtgacc atctcgagaa aatgcagggt tcctgactga
    16321 tggtactggc gcaaaaataa ctcgtacaca cctggattgg gaaacttcgg tgcttgcccc
    16381 aacccgccat tctccgcgtc gtaagcagtc gcaatttgtg cggcagcttt gccgattgtt
    16441 tccggggaaa aggcatgctc atgatcttcg gattgagaca tccgactgag agccgataaa
    16501 atttgctcaa cgctcttctg aacatcttcc ggtttgtctc tataagcctg cgccacgccg
    16561 tgcagaatac gcggaaatcc cggcatgccg tgacgatctt ccggcgggaa atatgtaccg
    16621 ccataaaacg gctgccgatc cggggtaaga aacatcgtca ttggccaacc tcccctgccg
    16681 gtcagcattt gtacagcatt catgtagatt tcatccaagt cggggcgttc ttcgcggtcc
    16741 actttgatgt tgatgaagag atcgttcatc agctgagcga tgtgctcatt ttcaaaagat
    16801 tcgcgctcca taacgtgaca ccagtggcag gcggagtaac caatgctcag tagaaccggt
    16861 ttatcctcgc gtttagcctt agcgaaagct tcgtcactcc acgggtacca atccacggga
    16921 ttatgggcgt gctggagcag ataggggcta gtctcatgaa tcaaacgatt tgtgtgtttc
    16981 gcatccattt ggatccctcc tttagtgacg ctaacacaga gaaaggcgtg tcaacaccta
    17041 accttggctg cggaatttac tcgcagccgg tcagcgattg attgggttgg ggatgcaggt
    17101 aatgagatta ggcacgacac ccgagatgca gaatagtttg agtgggcttg gaaaggattc
    17161 accgaatctt caagtcgaaa gcgacgggcc ctttctcccc ttttcgagtt tgaaattgca
    17221 ctgtgtcgcc ttcattgaag tcttcaatct gattgcgcgg tgccatgctg ccgcggtgaa
    17281 agaaaacctc ttcgctgcca tcatccggag tgatgaaccc aaagcctttg ggtttaatta
    17341 ttttcttgat agtgccagtc ataataattt cctcctcacg cgcgcttaaa gcgggattgc
    17401 atctcggatg cgactattga caatcaaaac acatttaact tagcgtgcaa atttaaaatt
    17461 catatagatc aaggaacctt gagttcagtt ctctactata tcaccggcca tggttacggc
    17521 cacgccgtgc gttcttgcca ggtcatttgg aagctgaagg agcgttgtcc ggaattgacg
    17581 gtccatatcc gcagcaccgt tccggagtgg ttgtttgagc atccgcacta ttctgtaaaa
    17641 caaagctcgc aggcgctcga cgtcgggatt gttcaagatg acagtctgaa gatggaactc
    17701 gggccgacgc tggaagcgtg caagcgttta acagcccgga tcccatcctt agtcgagcaa
    17761 gagctagatt ttataacaaa acagaacatc acccttatca tgggcgatgt cccgccgctg
    17821 tgttttgaag ttgctgcgcg agccggggtt ccttccgtag caattacaaa cttttcctgg
    17881 agcggcatct acagaggctt cttgcaagct ctcccggact ttctgcccct aatcgaagag
    17941 atggaggact tttatcgtag gacaacgctg gcgctgaccc ttccctatag ctgtgcagtg
    18001 gaaatttttc cgatgcgaga acggattcca tttattaccc ggtcttcagc attggcgcag
    18061 agagaagccc gagagagatt cggaatccca agcctggcca ccgttgtgct tttaagtttt
    18121 ggaggtcatg ggcttagacg attgcatctg gacagattac tacggcagaa agagtttttt
    18181 tttatcggta ctggtgatga gctgatgaag gaatccaact tgttgattct tcctgatgct
    18241 caacgtagct acgtggacct ggttcgcgct gcagatgttg ttgttagcaa acccgggtat
    18301 ggcattgtta gtgacattat cactcatcag gttccgctcc tctacaccga tcgcggcgag
    18361 tttgcggagt atccttatct ggtgaaggcc ttggaggact gcgcgacggc ggatttcatt
    18421 cctcaggagg atctcatatc cgctcatctt acaccttacc tgaagcggct tttgaccaag
    18481 gcgaagcatt ggccggcagt ttcattaaac ggcgctgcag tcgccagcga gaaagttttg
    18541 agcttactcg gataagcatt ttcggtaggt atgcgataga acgtgttgac agccatggct
    18601 gaatcatcca gattgagatc gttatggcag tcgaaaaggt cgttcttgca aagccgcgag
    18661 gattctgcgc gggtgttgaa atggctttgg caacagtgga gcgggccctt gagaaacacg
    18721 gtccgccgct ctatgtattt catgagattg tccataatca gcatgttgtt cgggacttca
    18781 tcgctcgcgg cgtgcatttc atccaatcca tggatgaagt gcccgccggc gcgagggtga
    18841 ttttcagcgc ccatggaatc tctcccgagg tatggcagca agcaggccaa aagcgtctcg
    18901 cctttgtgat cgatgccacc tgccctcttg tcgataaagt tcatcgggag gtgaaaaagt
    18961 ttgcatcgca aggatatggg ataattttag ttggtcatga gaaccatgac gagatagttg
    19021 gcaccagcgg tgaagcgcct gagcacgtta gggttgtcgc gaacatcaat caagccaaga
    19081 acgtaacagt gccggatcct gagagagtcg cagttctgac gcagacaact ctcagtgttg
    19141 acgacactcg cgagatcatc gatgtgctca ggcgaaggtt tccaagaatg gttacgccgg
    19201 cgaaagaaga tatttgttac gcgacacaaa accgtcagga tgcggtgaaa cagttaacgc
    19261 gcgcagtcga tctcgttctg gtgataggat cggaaaacag tgaaaattcg aatcaactat
    19321 gtgcggttgc ccgttcacaa ggaactcctg cgtatttgat cagtgaaatc gcgctgcttg
    19381 atcccaattg gctacaaggg gtgaggcggg tcggtataac ttccggtgcg tccgtgccgg
    19441 aacgtttagt aaatgaggcg gtcgactatt ttatacggca gggcgccgaa gtctcccacc
    19501 taggttttat cgaagagaat atccatttcg ccttacccac cgaagttgtt cacccacaat
    19561 gaatgcgaat tgaagcaggc gcgtcccaga tctctttgct ggactgttca ctcaagcgtc
    19621 actcgttgag tttttccgcg tgcctcacaa ctgtgcaatt gattaagtcg tgagcagtgg
    19681 gttgattatc gcttcagcaa gttccaccaa aactcacgga atctctggag gaatctatca
    19741 gcttaggtgg cattttcatt gcgtttctct tgccaggtac cctacgcctt attgctcggc
    19801 ctagccaaga gagaggttcg tcgtgatgca ccataccaaa gcaataattt taattctttt
    19861 ttttgcttca acaatcccgc tgacagcagc gctttcccag gaagtgaagc cgtctggtgg
    19921 cgcgccggat acagccatca ccgcgccgac agcggctccc gagccgccag ttgacaaagg
    19981 agatacagcc tggattctaa cctcttcagc gctcgtgttg atgatgacca ttcccggatt
    20041 gtttttattt tacggtggac tggtgcgggc caaaaatgtt ctcggaacca tcatgcagag
    20101 cttcgtggtc gtcgctctca tcagtatcca atgggttttg tggggttata gtctcgcctt
    20161 cggaccggat gtaggtggct ggattggtag cctagcttgg gtcggattag atagcgttgg
    20221 cgcaactcca aatccagctt atgcggctac gattccgcat caagcattta tggttttcca
    20281 aatgatgttc gcgattatca cgccggcgct catcaccggc gcgattgcgg aacggacaaa
    20341 attcgggaca ttcatcgttt ttgtgctgtt gtgggccacc ttcgtctacg atccgctcgc
    20401 gcattgggtt tggggcaagg gatggatggg ggctatgggg gcgcttgatt ttgccggcgg
    20461 cacagtcgtg cacattagct ccggattttc ggcattggcc gcagcgtact ttcttggaag
    20521 acgtgtcggt tacggatatg aaacgttgac gccgcacaac ctgcccttta ccgtgatcgg
    20581 agcctctcta ctttgggtcg gttggttcgg cttcaatgcc ggtagtgctt tggctgcaga
    20641 cggcttagct accatagcgt ttgtcacgac caatacagcg gctgcggccg cggccctcgc
    20701 ttggatgttt actgaatggt ttacccatgg caaacccacg gctctcggcg ctgccagcgg
    20761 tgcagttgcc ggacttgttt cgatcacacc ggcggccggc tttgtcggcc cgttgtcggc
    20821 gatcatcatc ggcgcggttg ggggtatgtt gtgctataaa gcctgcaatc taaaagccgc
    20881 ccttggctat gacgattcgt tagatgtggt cggcgtccat gggattggcg gtacatgggg
    20941 agctctagcc accggattgt ttgcctcgaa agccattaat cccgccggta atgatggtct
    21001 cttctacggc aaccctgcac aatttggtgt ccaggccgtc acggttgtgg cgacgatcgc
    21061 ttttgcgctg ttaatgacat tcatcattct agcaattttg aaagctacca tgggcctacg
    21121 ggtatctgaa gaagaggagc gtatgggtct ggatttaacg caacacaacg aacgtggcta
    21181 cgcttaagat cgcacccagg gctagctgga cttcggataa tgtgataaca gattgacgat
    21241 ttcgatgtgc taaataggac gaaggagtag atagatgcca gttaaagacg ctttggagat
    21301 ggcaaagaag aacaaatcgg aaattgtgga ttttaaattt atcgatcttc cgggactatg
    21361 gcagcatttt tctatcccgg ctacagaatt gtcagccgac atatttgagc aagggttggg
    21421 atttgacggc tcgagcatca ggggatttca gagcatcaat gaatccgaca tgattctgtt
    21481 gccggattcg gacacggcca tgatggatcc attcagagag cattcaacca ttagctttgt
    21541 ctgtaatgtt ttggatgcga tcacccgtga gccttacagc cgcgatcccc gatttgtcac
    21601 gcagaaagcc gagaaatacc tgaaatccag cggtatcgct gatatcagct actggggtcc
    21661 cgagattgaa tttttcatct tcgacagcat ccggtttgat caaaatcatc atgaaggcta
    21721 ctaccacatc gactcggccg aagggttctg gaactcgggc aacaacgatg gtcccaatct
    21781 cggcaacaaa atccgctaca aggaaggcta tttcccgatg ccgcccatgg atcagtacca
    21841 agacctccga tcggagatgg tgctgaatct gcagaaatgc ggcattacag tcgaagtaca
    21901 tcaccacgag gtggcgaccg gcggtcagac cgaaatcgat atgcggtacg atacgatgac
    21961 caagatggcg gacaatgtgt tgctatacaa atatgtggtt aagaacaccg cccgacgccg
    22021 cggtaaagtg gtcacggtca tgcctaagcc actttttcag gataacggtt cgggtatgca
    22081 ctgccaccag agtttatgga aaaatggcaa gaatctgttt tacgacaaga agggttacgg
    22141 cttaattagc gacacagcgc gttattacat tggaggttta atcaaacatg cccatgcgct
    22201 ctgtgccttc atcgccccta ccacgaactc ttacagaagg cttgttcccg gctacgaagc
    22261 gccgatcaat ttagtctatt cggcccgtaa ccgcagcgcc tgcgtgcgta tcccaatgta
    22321 ttccgacagt gagaaggcca agaggctgga atttagaacc cccgatccga gctgcaatcc
    22381 atactttgca tttcccgcga tgttgatggc gggacttgat gggattcgta acaagatcga
    22441 gccgccgaaa ccgattgaca agaacctgta tgagctcgag cccgaagaag ccgctaaggt
    22501 gaaatcgatg cccggatctt tggatcaggc actcgatgcg ctagagaaag atcacgattt
    22561 cttgcttaag ggcgatgtgt tcaccaagga cgtgatcgat acctggctag agtttaaacg
    22621 caaaaatgag gttgacgcta tccgccttag gcctcatccc tgggagtttg ctctttattt
    22681 tgatatctaa gtggggtgag gctggtcgct cttgccgatt taagagttca cgatcagcag
    22741 cattaggagg cggatatgat gaaaaaaatc gaagcgatca taaagccatt caagttagat
    22801 gaagtcaagc agcggctaac cgctgctggg attaccggaa tgacgatcac cgaggtcaaa
    22861 ggtttcggcc ggcaaaaggg gcacaccgaa ctttaccgcg gcgccgaata cacggttgac
    22921 tttcttccta aggtcaagat agagattctc gcgccggagg agatggcgcc gaagattgtc
    22981 gaagcaattc tggagtccgc gcagaccggc aaaatcggcg atggcaagat ctttgtctca
    23041 tcggttgaag aagtgatccg tatccgaacc agcgagaggg gtgaagaggc aatcagttag
    23101 ttcactgctc taatctcgtc gcagtgcttt aggtacccgg cctgcaattc cggatggcca
    23161 tggctcttgg taatgattgt gcaggcgatc cggaattgcg tgggacgg
//
LOCUS       DASUVS010000255         7871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_977012, whole genome shotgun sequence.
ACCESSION   DASUVS010000255 DASUVS010000000
VERSION     DASUVS010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7871
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977012"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..759
                     /locus_tag="VF182_14110"
     CDS             295..759
                     /locus_tag="VF182_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEX6438269.1"
                     /translation="MEKANRKRRIYSVRNSPIHGRGVFALVPIPKGTRIIEYRGELIT
                     DDEADRRYADLHEHSPHTMLFSLDNNLVIDATRRGNSARWINHSCAPNCQVEEEGTRI
                     FIDARRDIRPEEELTYDYNLQLGERHTRAAKRAHACFCGTRRCRGTMLGSRI"
     gene            1073..2329
                     /locus_tag="VF182_14115"
     CDS             1073..2329
                     /locus_tag="VF182_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438270.1"
                     /translation="MTSKTKKIRGASIEPPESRAKTLFSFIQPSVLEFVTSNAVTNLI
                     IFGLLLFFSVSCATINTEVPERTETPSRFIEGFQTSFDSRFKHLAEIQDVGKPLENSF
                     SLNLGPQLLKVKASYLEKSESTNAMTVASPQPSTRRYLNLVGSTSFGGTNLSGDGELT
                     YGSGNISPAGCDCLDWPRMMRLGIKTSWQGLSYGADFKSIEKGFIPISGAASAEERDE
                     GQLWGEYNLGPIHLRGSLVESWEELLDANRHRVTRAATTAMQLNRSVWSGTLTSSYGL
                     VEQGPDLNQEMRVFSNRLTGLYRPFSVLSLGPSIGIKHEWDTVTGYRTETPMTEFAIV
                     YTPLQDKFRLVGGTSFTRTVNRYALTDVTTIGTRAIMDWKIGKFLGREDIVSLNVNYN
                     RQRDLISSGKSYDDVSGILQLKVTGF"
     gene            2777..3022
                     /locus_tag="VF182_14120"
     CDS             2777..3022
                     /locus_tag="VF182_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438271.1"
                     /translation="MKTTVLGFLVGAIFTIVITAVAALDAFAAGGLVLLASVVAGLAA
                     GLCIGGLIAANFAMLATGEKETEEVIAHSPNAAGAAA"
     gene            complement(3303..4298)
                     /locus_tag="VF182_14125"
     CDS             complement(3303..4298)
                     /locus_tag="VF182_14125"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438272.1"
                     /translation="MIPRTLLARIRLMIIVSIAGVSVPTYCNAAEKLVGLQSAPSMAM
                     ALPWVAEEARLYSKYDLDFQLVYIASSGIVTAAMSSGSGAVAIVGGEGPIRAFLGGNT
                     DFVFIGSVKNVLTHSIMGKPDIKKPEDLKGRRIGVGRIGGNSHYFAQYGFRQKGLDLT
                     RDANFIQTGGAPETFQALASGAVDAASLTTPQDTRAAFMGYNYVIDGRELKPPYIATG
                     FVTLRSVIGKRPKVVSQFMHAMAESLKLMMTDRELAYRLMAKKIGLTDRKVFDAAYTA
                     EMKVLEPRLDIKPIAIRATLEEIAKTDPRARQVTASQLIDRRFLDEMEKDGTFGR"
     gene            complement(4468..5622)
                     /locus_tag="VF182_14130"
     CDS             complement(4468..5622)
                     /locus_tag="VF182_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEX6438273.1"
                     /translation="MLVVFNLGFRPFFLLSGMFAVILMGLWVPSFIGGRALRTYYGQI
                     SWHSHEMIFGYAAGVIAGFLLTAVRNWTGLATATGSSLAALVALWLLGRILPFFVETI
                     PGWLISVVDLAFLPALTASIAVPLIRHAEKRNLLFLPLMLALFAGNLLVHLELLGGVP
                     GVARYGIFLGLYLIILLIVIMGGRVIPFFTGRALSGVVIKRRPLIEWLAPVTVIAFMF
                     AELLFPNSQVAGVLAGLAAIINAIRVVSWYSHRCWQVPLLWVLHLGYGWIVVGFLLKA
                     AAAFGLIAAQFTIHAFTVGGIGVLTFGMMARVALGHTARPLRVGSSMAVAFALLNVAA
                     IFRGLLPAFLPLWFSQLILLSGLLWIAAFLVFVIVYMPILTQSRIDGQPG"
     gene            complement(5681..6670)
                     /locus_tag="VF182_14135"
     CDS             complement(5681..6670)
                     /locus_tag="VF182_14135"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438274.1"
                     /translation="MFSAVVSLAFIISLLVINPSLISAQERVRVGWAAMTASHTPLWV
                     AQEKGFSAKQGLSVESIFFGAGPPAMQALVAGDLDIVITSAPNVVNPRLGGAETVMIL
                     SIIPTFIDHIISAPNIATPEQLRGKIGSVNRFGSISDMGLRLSLRRLGIDPEKDVRIV
                     PAGGNPERLAAISKNISQFTIMNEPFIKEAERLGYRDLVNMATLKIPLHGNGIVTTEL
                     IIKSKRPIVTKFVRAITEAIHYVKKDKEGTKTVIGKYTKLTDPEGLERTYKNYTSVLL
                     EVPYADPTGIKTLLDDMAPRNPKAASADPKAFVDPSFVHEMESSGFIKQLYKR"
     gene            complement(6880..7416)
                     /locus_tag="VF182_14140"
     CDS             complement(6880..7416)
                     /locus_tag="VF182_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6438275.1"
                     /translation="MISPPETFESQRLQLRKPLLADAEEIFRQYAQDAEVTKYLTWLP
                     YEKVDETRAFVRTCLLMWQQGDSYHWTLVRKGDGQLLGMINARVDGHKWELGYVLARE
                     YWGRGYMTEAVKRLIATAFEEAAVYRVWAVCDVDNVASARVMEKSGMQREGLLRRWSV
                     HPTSSAEPRDSFCYSIVR"
BASE COUNT         1933 a         2132 c         2057 g         1749 t
ORIGIN      
        1 cggattgtga cagcttcgta gcgaaaaccc atctcacctc ctgctgacgt gatgagctca
       61 tacatgatca gtgctaccct cagcttaggc acgtggaaac gctccttttc atcgcagtgg
      121 atgtgatatt tgccaacgcg ctcgaatcgt atcgcaagga atctgtgtga gtgtcgcccg
      181 ccgctaagac ccggtgggtt cacaaggctg gcgtacggga aactcgattg taaaaagcaa
      241 ctccgcaggt cttttttgat agtcaactaa aaataaatat gattgtgtaa gcatatggaa
      301 aaggcaaatc gcaagcgacg aatttattca gttagaaatt cccccattca cggccgcggc
      361 gtctttgcgc tagtcccgat tccaaaagga acgcggatca ttgaatacag aggcgaatta
      421 ataaccgacg atgaggcgga ccgaaggtac gctgatctgc atgagcactc gccccatacg
      481 atgctcttct cgcttgacaa caatctggta attgacgcga cccgtcgcgg caactccgcc
      541 cgctggatca atcactcctg cgcccccaat tgccaagtcg aggaagaggg cacccggatc
      601 tttatcgacg cccgtcgcga catccgcccg gaagaggagc tgacttacga ctataatcta
      661 cagctgggcg aacggcatac tcgcgcggca aaacgagccc atgcttgctt ttgcggtaca
      721 cgccgatgtc gcggtacaat gctgggctcg cgaatttgat tccgccgctt gttctcagtc
      781 gtggtaccct gtcgaaaagg tatatttaaa aaacgtactt atgtactcat cgaattgtgt
      841 ttatagcacc gcggagcagc atttagttga gcagctgagc ccgtcaactt gatccttgct
      901 tgttgtcgca cgcgcggtga tatgcccggc ttacagcaaa gccgaccgct tgtaggtgct
      961 tccttaagtt caactttggg ggttcgttaa gcctgctaaa ggtgtgactg tttgtctgca
     1021 aaacttacct gaatgatctt aatcacggcc caggaaccta gatctcatat tagtgacttc
     1081 aaaaaccaag aaaattcgcg gagccagtat tgagccaccg gaatcccggg caaaaacatt
     1141 attttcgttc atccaaccca gtgtgttaga atttgtcacg agtaacgcag tgactaatct
     1201 tattatcttc ggacttcttc tattcttttc ggtttcgtgc gccacgatca ataccgaagt
     1261 tccggagcgt accgaaactc ccagcagatt catcgaaggt ttccaaacga gctttgatag
     1321 tcgttttaag cacctggcgg agattcaaga tgtgggtaaa cccctggaga atagcttctc
     1381 gttaaacctt ggacctcaac ttttaaaagt taaggcgtcc tatcttgaaa aaagtgaatc
     1441 aaccaacgca atgaccgtcg cttcacccca gcctagtacg cggcggtatc tgaatttagt
     1501 cgggtcgact tcgtttggtg gaaccaatct gagcggcgac ggcgaactga cctacggctc
     1561 aggcaatatt tccccagccg gctgtgactg cttggattgg ccacggatga tgcggttagg
     1621 aataaaaacc agctggcaag ggctaagtta cggggctgat ttcaaatcga ttgaaaaagg
     1681 atttatccca atttcaggag ctgcaagcgc cgaggagcgc gacgaaggcc agctttgggg
     1741 agaatacaat ctgggcccga ttcacctacg cggctcgctc gtggagtcat gggaagagct
     1801 tctggatgca aatcggcatc gcgtcactag agccgccaca accgccatgc aattgaaccg
     1861 atcggtgtgg agcggaactc tcacctccag ctatggattg gtggaacaag gcccagatct
     1921 aaatcaggag atgagagtct tcagcaatag gcttaccggg ttataccgtc ccttcagcgt
     1981 attatcttta ggtccgagta tcggcattaa acatgagtgg gatacagtta ccggttatcg
     2041 aaccgaaacc ccgatgaccg aatttgctat cgtttacacg ccattgcagg ataagttcag
     2101 gttagtaggc ggcacctcat tcaccagaac cgttaaccga tacgcgctta ccgatgtaac
     2161 aactatcggc acacgagcca ttatggactg gaaaatcggc aaattcctgg ggcgtgaaga
     2221 catcgtatca ttgaatgtta actacaaccg ccagcgcgat ctcatctcct cagggaagtc
     2281 ttatgacgac gtttccggta tcctccagtt aaaagtcacc ggattttagc cccggttcta
     2341 ttcctctacg ccgaaccata cccgttctca gcgacgcatg tcgagacatc ggcgcgattt
     2401 cgtggggcgc cgattctcgc ccagcgagtt tccgcatacg ttaaaacaac gtcgaatttt
     2461 taagcgtgcc accacatccc ggtttgctgg ctcatgcgct ccgagaacct tttgctttcg
     2521 cgggcacatg aagtcgacat atgtcagacc atcgttaagg ttccgcaggg atccaaccaa
     2581 agcaacaatc caaggtgcgg cgatcaaata gactgcgaaa tttcattctt atggttcgga
     2641 ataccgcgtc tactttttcg cgcggttgag aaatatccgg ttactagcga tcgattttat
     2701 ggccctattt gcgagatcgg tttggcgcat gttttgcgct agcattttat catcacgacg
     2761 cacgaaggag atcctaatga aaaccacggt gttgggtttt ttggtcggcg ccatctttac
     2821 aatagtcata actgcagtag cggcgttaga tgcctttgcg gctggcgggc ttgttctgct
     2881 ggcatcggtg gtcgccggtc tggcagccgg cctttgtatc ggcggcttga ttgctgcgaa
     2941 cttcgcgatg ctggcgacgg gagagaaaga aacggaagaa gtcatcgcac acagcccaaa
     3001 cgctgcgggc gcagcggctt aacgggatct tccacgacca gccggtcggc agcgatgatc
     3061 ctgagtgccg actggttggc gccatcttca acttcagaat ttctgagcca gataacgaaa
     3121 gctcctcgca acaccgggcg agccaccgat ttcgctgtag gcacgccctg atcctgcgtc
     3181 atatcggcca gcggatatcc gtacaccctt ccgacgcaaa acagctatgt cgtgattagg
     3241 ccacgatcta agaaattgtc ctgctccggc ttcaacgttg aaccttttgc gaattttatt
     3301 gtttaccgcc caaacgtccc gtccttttcc atctcgtcca gaaaccggcg atcgatcaat
     3361 tgcgatgcgg tcacttgcct ggcgcgcggg tctgttttgg cgatttcctc gagggttgcc
     3421 cgtatcgcga tcggcttgat atccagccgc ggctctagaa ctttcatctc ggcggtataa
     3481 gcggcatcga agaccttgcg atcggttaaa ccgatttttt tcgccatcaa ccgataagcc
     3541 aattcccgat ccgtcatcat gagcttgagc gattctgcca tggcgtgcat gaactggctg
     3601 acgaccttcg gccgtttgcc aatgaccgac cgcaacgtca cgaatcctgt ggcgatatac
     3661 gggggcttta attcgcgccc gtcgatcacg taattgtaac ccatgaaggc cgctcgcgtg
     3721 tcttgcggcg tggtcagcga agccgcatcg actgctcctg aggctaacgc ctggaacgtt
     3781 tcaggcgcac cgccggtctg tatgaagttg gcgtcgcgag ttagatccaa gcccttctgg
     3841 cgaaagccgt attgggcgaa atagtgcgaa ttgccgccga tccgtccgac tccgattcgc
     3901 ctgcccttta aatcttccgg tttcttgatg tcgggcttac ccatgatgct gtgagtcaag
     3961 acgtttttca ccgagccaat aaagacaaaa tcggtattgc cgcccagaaa agcacgaatc
     4021 ggtccttcac cgccaacgat agccacggcg ccgctgccgc tgctcattgc cgctgtgacg
     4081 atgccggatg aggcgatata gaccaattga aagtcgagat cgtatttcga gtaaagccgc
     4141 gcttcctcgg caacccaagg cagcgccatg gccatgcttg gcgcggactg caggccaacg
     4201 agtttctccg cggcgttgca ataagtagga actgaaactc ccgcgattga aacgattatc
     4261 atgagacgaa tacgagctaa caacgtacgc ggtatcataa taaacccgcc ttagagagta
     4321 gagataagaa tgttgattta ggagcgcatg attctgacct ttggtgcaac ggttggatta
     4381 cgagcactgc cgcacgcttg tgcgcatcca cccagagcac gaaaggtccg gactttttac
     4441 tacaggtgat gccgtttgcc caggctctca tccgggctgc ccgtcgattc tggactgggt
     4501 aagaatcggc atgtagacga tcacgaatac gaggaaggcc gcaatccaga gtaggccgct
     4561 taacaagatg agctgagaaa accagagcgg aaggaacgcc ggcaacagac cgcggaagat
     4621 ggcagctaca ttgagcagag caaatgcaac tgccatggat gatccgactc tgagaggcct
     4681 ggccgtgtgc ccgagagcga cccgggccat catgccgaag gtcagcacgc cgataccgcc
     4741 gaccgtaaac gcatgaatcg tgaattgcgc cgcgatcagt ccgaaagctg ctgctgcctt
     4801 aagtaagaaa ccgaccacga tccagccgta gccgagatga agcacccaca gcaaaggcac
     4861 ttgccaacaa cgatggctgt accagctcac aacgcggatg gcgttgataa tggcggctag
     4921 cccggccagg acacccgcta cttgcgagtt cgggaacaga agctcagcga acataaacgc
     4981 gatcacggtc acaggcgcga gccattcgat gagcggccgg cgcttgatca caacaccgga
     5041 caaagcgcgc ccagtaaaga aaggaatgac tcttccaccc ataatcacaa tcagcaggat
     5101 gatcaaatag aggccgagaa atatgccata tcgagcaaca cctggaacgc cgccgagaag
     5161 ttccagatgc acgagcaggt tgccggcaaa gagagccaac atcagcggca aaaaaagcag
     5221 attgcgcttc tccgcgtggc ggatcagggg cacagcaatg ctggcagtta gcgccggcaa
     5281 gaacgccaaa tcgacaaccg agatcaacca gcccggaata gtttcgacaa agaagggcaa
     5341 tatccggccc aggagccaca gggcgacaag agcggccaaa gaactgccgg tcgctgtcgc
     5401 caggccggtc cagttgcgca cggcagtcag cagaaagccg gcgatgaccc ccgctgcata
     5461 gccgaagatc atctcatggc tatgccagct gatctgtccg tagtaggtgc gcaaggcgcg
     5521 cccaccgatg aaggaaggaa cccacaggcc catcaagatg actgcgaaca tcccgcttag
     5581 aagaaagaat gggcgaaagc cgaggttaaa tactaccaac atcgagtctt tcaaacttgt
     5641 agagaagcag catcggcgcg cttagctcct tcgagtcacg ctagcgctta tagagctgtt
     5701 tgatgaaccc tgacgattcc atttcgtgca caaaactcgg atcaacaaac gccttcgggt
     5761 cggccgatgc ggctttggga ttgcgcggcg ccatatcgtc aagcaaggtt tttatgccgg
     5821 tcgggtcggc atagggcact tccaacaaaa ctgaagtgta atttttgtag gttcgctcca
     5881 gaccttcggg atcggtcagt ttggtgtact tgccgatgac tgtcttggtc ccctctttgt
     5941 ctttcttgac gtaatgaatg gcttcggtga tcgcacggac aaacttcgtc acgatcggcc
     6001 gcttgctctt gatgatcaac tccgtggtca cgatgccgtt gccgtgcagc ggaatcttca
     6061 aggtcgccat gtttactaaa tcgcgatagc cgaggcgttc ggcttctttt atgaaaggtt
     6121 cgttcataat agtaaactgc gagatgttct ttgaaatagc cgcgagccgc tccgggttgc
     6181 cgccagcagg gacaatcctc acgtccttct ccggatcgat acccagccgg cggagtgaaa
     6241 gccgaagccc catatcggat atgctgccga aacgattgac cgaacctatt tttccgcgca
     6301 actgctcggg ggtcgcgata ttcggcgcgc tgattatatg gtcgatgaag gtcgggatga
     6361 tcgacaatat catcaccgtt tcggcgccgc cgagccgggg attaaccacg ttaggtgccg
     6421 aagtaatcac gatatcgaga tcaccagcga ccagcgcctg cattgccggc ggaccggcgc
     6481 cgaaaaagat cgactcgacg gacagaccct gcttggctga aaatcctttt tcctgcgcga
     6541 cccaaagcgg cgtatgcgaa gcggtcatcg ccgcccagcc aacccgcacc cgttcctgag
     6601 ccgagatgag agacggatta atgacaagca acgaaataat gaatgctaaa gacacaacgg
     6661 cgctgaacat gaacttcacc tcacaaaaac tcttgatctg aatgtcgcac gatcccaagc
     6721 gcaccgatgt ccattcgcag gccatcctaa tggacggttt tcagaataac aaggaatcgc
     6781 tcgccgtcag ggcaatgcga cagggccggg aaaaaataaa gatgacggaa gatgtgtggc
     6841 tatcgtaagc cgcttaactg atcgagcagc gctgggcaat catctgacga tcgaataaca
     6901 aaacgaatcg cgcggctcgg cgctggaggt aggatggacc gaccaacggc gcagcagtcc
     6961 ttctcgttgc atgccggact tttccatcac gcgggccgac gccacattat cgacatcaca
     7021 cacggcccag acgcggtaaa ccgccgcctc ttcgaaggcg gtagcgatga gcctcttgac
     7081 cgcctcggtc atgtatcctc tgccccaata ctcccgcgcc agaacatacc cgagttccca
     7141 cttgtggccg tcgactctgg cgttgatcat gccgaggagt tggccgtccc ctttgcgcac
     7201 cagtgtccaa tgataggaat cgccttgctg ccacatcagc aggcacgtcc ggacgaacgc
     7261 gcgagtctcg tcgacttttt cgtaaggcag ccaggttaag tacttggtga cttcggcatc
     7321 ctgggcatat tgccgaaaaa tctcctccgc atcagccaat aacggttttc gcagttgcag
     7381 ccgttggctt tcgaaggttt caggcgggct aatcacccaa cgcagatcca gcagacatga
     7441 tcgtcaagtt taggttcgct tagaataata ttcaagagtt ggttacccca gctccggtag
     7501 atacaacgtc tttggtcgag gatcaatgtc ggcaacgcca tcaatgttca ttccaggcgg
     7561 gatcgataaa cttctaggca cggcaagcat gcaaatacat gatcaatcga catgccgcag
     7621 acttcgtcgc tttccattgt catttcccgg cacagtgggc aactatgcag gtgctttttc
     7681 gggggggaac ttgatgtttc gatttttgaa acgtcacaga gcgggattat cttagccttc
     7741 atttcacgat tgtctctcag tcatggcgcc gagcgcgatg ctcaaggtgg cgcgagcgcg
     7801 atcacccacg agagcaacgt gaatgccaag ctacgagacg atctcacagc gacaaatgat
     7861 tagattgggg t
//
LOCUS       DASUVS010000256         8186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1852799, whole genome shotgun sequence.
ACCESSION   DASUVS010000256 DASUVS010000000
VERSION     DASUVS010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8186
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852799"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VF182_14145"
     CDS             complement(<1..197)
                     /locus_tag="VF182_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEX6438276.1"
                     /translation="MSKTSSAGNVRSAPALREMVEEFLYQEAALLDAWRLDEWLALFT
                     ADGRYFMPTTDLPDGDPRKDLV"
     gene            complement(212..1519)
                     /locus_tag="VF182_14150"
     CDS             complement(212..1519)
                     /locus_tag="VF182_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX6438277.1"
                     /translation="MFDPENLIVDKPQEGVFRVRRSAMTSAEVFRMEQERIFNKCWLY
                     LCHESEVENAGDYRRRTVAGRPLFFIRGSDGKLRVFLNTCTHRGALICRHDQGHADVL
                     QCFYHAWSFNNHGDLVGVPDEAGYPGSFNRAAMGLKSPPRVESYRGFVFVSFNPDVED
                     LVTYLAGARDYLDLIVDQTEKGMRIIPGSNRYSIKANWKLLVENSLDGYHVRPTHKTY
                     FEYVNSLGINTSGVQTAFSRPAVVLGNGHAVEEHDGIRGRPIARWSQLFGEENKEEIA
                     AIRAGLVRKFGEQKARHMADISRNLLVYPNLLIIDSIDITVRIFWPLAPDLMEVTAWQ
                     LAPKEEKPALLARRLDNFLTFHGPGGFATPDDVEAIESCQAGYKAEGVEWSELSRGMH
                     REAIAEDELQLRAFWRQWFGHLSGMGKVETNDPVIRERAALAR"
     gene            complement(1846..2823)
                     /locus_tag="VF182_14155"
     CDS             complement(1846..2823)
                     /locus_tag="VF182_14155"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX6438278.1"
                     /translation="MSKLKLTLACWDYDRTRPLIDGRVQAEGIDLEIKVMRPRETFPR
                     MLEDKEFEVSELSLGSYVSLIGRGACPFVAVPVALSKIFRHSCIYVRTDAGIRTPQDL
                     RGKRVGTTQLGSTATIFMNGMLQHEYGIAMQDIQWFIGGLTTPTQRPLIPLKLPDKVK
                     VEFLPVERTLEGMLESGDLDALLGIYIPSIFEKGSPRIARLFPNYYEVEQDYYRRTGI
                     FPIMHTVVLRDDIYREHPWAARSIYKAFCKARDLAIDGLYDTDALRLSLPWLIHHIEE
                     TWRVLGKDFWAYGLEPNRPALQAIGEYVYEQGLSPGVVSPNELFAADVE"
     gene            complement(3106..4119)
                     /locus_tag="VF182_14160"
     CDS             complement(3106..4119)
                     /locus_tag="VF182_14160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6438279.1"
                     /translation="MRFGIEFGSYPADLDSAEVCEQVSLRAQAAYQNNFEALFVAQHY
                     LTGPDAAILQSIPLLAYLAAQVPGMYLGTSIFLLPLHHPVMVAEYISTLDNLSGGKVL
                     FGVGQGYRENEFNSFGIDLRQRRSRLVEALDVIRKLWTGEPVTFHGKHFHLDNVMSLP
                     TPVQRPGPPILMGADTVRTVARVPEVADHWIASRRHSKSFLRQALPEYKAALERQGKP
                     YRGLYIFRDLCIADTTREAEERIKEGYERRYRRYQQWGQPGERYDLNFDELKYDRLIL
                     GSPSEVVEQVMAYHEEFGAEVMWFMVDWPGMDRRFTLETIERFGREVIPQIKRLTPES
                     PLP"
     gene            complement(4264..4893)
                     /locus_tag="VF182_14165"
     CDS             complement(4264..4893)
                     /locus_tag="VF182_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438280.1"
                     /translation="MAKTITIPVDVYLITLQIESYQVVEKKIAVGKSTQIQHERVKET
                     RDIPPNQNDKTVMRAIDVANTIWAPADITFQLRKSFFPATEAPGNVEEVSVSGFFQMI
                     TALKIKPIGAVAALFVNRFEKRDLGGFAAEALGVCIVTSLGDPVLGKTLAHEFGHLLS
                     LPDLRNDPKSVANNYNLMYEASRAGDKLTSDQISKARNSERVKLVYQSP"
     gene            5274..6302
                     /locus_tag="VF182_14170"
     CDS             5274..6302
                     /locus_tag="VF182_14170"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6438281.1"
                     /translation="MKRLEITLAFMTLFMGFGSNASAQDFYSNKTLRIVVGFSPGGGM
                     DIYARAIGRHLGKNIPGKPTMIVENMPGAGSLIAANYLYNQAKPDGLTIGSWIGGLVL
                     QQVIGGQKGIEFDARKFEWIGAPAGDSSSCAVSQSSGISTTDQWLAAKTPVKIGAMAP
                     GSVTSDIPKILKAALHLPIQIVEGYKGTAEIRVAADSGEVDGGCWGWETIKIMWRQGL
                     DSGSTKVLIQMLPKRHPDLPDVPNALELAKNDDARQLIKIGIVDPATMVRSLTLPPGT
                     PKDRVKILRDAFMATMKDPQFLAEAKTAKLDLNPLPGDEVEKIVHGFFSLPPSVATKL
                     RDIVVSKN"
     gene            6327..6707
                     /locus_tag="VF182_14175"
     CDS             6327..6707
                     /locus_tag="VF182_14175"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6438282.1"
                     /translation="MQPMFIQHEPKRVYSKACVCGNFIFLAGEDCKDPKSQAIRGTTV
                     PEQFDYTYQNMKETLESLGSSLENIVSLTTYVTDPRARQAHQRARAKWLPQNPPSALI
                     CGVQLADPEMLVEIQGVAIIPEKK"
     gene            6793..8100
                     /locus_tag="VF182_14180"
     CDS             6793..8100
                     /locus_tag="VF182_14180"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6438283.1"
                     /translation="MDALQQRIINEISQDELVAMTVDLVNFYSPTGGEAAIADYLAGR
                     LKELGMRVQLQEVEPGRNNVIGRLPGKIGNPTLLFSGHFDTSTTGREAEAWGGDYSAE
                     DFGGGQIRAAVTNGWISGVGASNMKGAFAAYWSALRALQRTGVELVGDVLVTGVVGET
                     EKAPVDQYKGGEFRGGKAGSRYMVTHGVTADYAIIGEPTGMRLQIGETGYCFAKITVY
                     GKSQHTWCKEFGIDPIEKMMRVIAALKAWEPIYQRRHPHAFMEPRIGIGAIQGGYPYK
                     PSKCPAPFCNLYVDLRLVPGQSFMETKRELEAVIADLKANDPDLRAEVHFYLTGNGYE
                     LERDSAVVIAMERAHREVYGEAISYAAPSRYAVSSDAGPMFEYGIKGLTYGPGGVSAA
                     GSFTVYDPSQQQSEVLSVENLVKAARVYALAALDLCGSNLNTQ"
BASE COUNT         1876 a         2282 c         2242 g         1786 t
ORIGIN      
        1 accagatcct tgcggggatc tccgtccggc agatcggtgg tcggcatgaa atatctgccg
       61 tcagcggtga aaagtgccaa ccactcatcc aaccgccagg cgtccaaaag cgccgcctcc
      121 tgataaagaa actcctccac catctcgcgc aaagcgggtg cgctacgaac gtttccggca
      181 gagcttgttt tcgacatgat tgcgttcgtt gttaccgggc gagcgctgct ctttctctga
      241 tcaccggatc gtttgtttca actttgccca tccctgagag atggccaaac cactggcgcc
      301 agaacgcccg gagctgaagc tcgtcctcgg caatcgcttc ccggtgcatg cctcgggata
      361 attcggacca ttcgacgccc tcggctttat agcctgcttg acaggattcg atcgcttcga
      421 catcgtcggg cgtcgcgaat cctcccggcc catgaaaggt gagaaaattg tccagccggc
      481 gcgccaggag cgcgggcttc tcttcctttg gcgcgagctg ccacgctgtc acctccatca
      541 ggtccggagc gagcggccag aaaattctta ccgtgatatc gatggaatcg atgatgagga
      601 gattaggata gaccaggaga tttcggctga tatcggccat gtggcgagcc ttctgctcgc
      661 cgaatttccg caccagccct gcgcggatgg cggcaatttc ttccttgttc tcttcgccaa
      721 acagctggct ccaacgagca atgggccggc ccctgatgcc atcgtgttcc tccaccgcat
      781 gaccgttgcc gagaacaacg gctggccggc tgaatgcggt ttgcacaccg ctggtgttga
      841 tccccaaact gtttacatat tcgaagtagg tcttgtgcgt cggcctgaca tggtagccat
      901 cgagactgtt ctcgaccagg agcttccagt tggccttgat ggagtatcga ttagagcctg
      961 ggatgatccg catgcctttt tcggtctgat cgacgatcag gtcgagataa tcgcgagcgc
     1021 cagccagata agtcacgaga tcttcgacat cgggattgaa gctcacaaag acaaagccgc
     1081 ggtaactctc cacccgcggc ggcgacttga ggcccatcgc cgcgcggttg aaactgccgg
     1141 ggtagccggc ctcgtccggc acgccgacaa gatcgccgtg attgttaaaa ctccaggcat
     1201 gatagaagca ttgcaaaacg tcggcgtggc cctgatcatg acggcaaatg agcgcccctc
     1261 tgtgcgtgca tgtgttcagg aacacccgca gcttgccgtc gctgccgcgg ataaaaaaaa
     1321 gcgggcgccc cgctacagta cgacgccggt agtcgcccgc gttctctact tcggattcgt
     1381 ggcacaaata aagccagcat ttattgaata tccgctcctg ctccatccga aaaacctcgg
     1441 ctgatgtcat ggcggagcga cggacgcgaa atacaccctc ttgcggcttg tctacgatta
     1501 gattttcagg atcgaacatg gtgtgcctcg ccgaacagtg cgtttgaccc tcatcctatt
     1561 ctacggtgca gcatcatggc aagaacagat gctgtggtga agtctcggat aatcaagcac
     1621 agagtcacag agagcacaga aagtactttc ttctcctaac tctacgctct ccttcctttg
     1681 cggtgaattt ttgattcatc tcctaacttt gggctcttcg tgcctccgtg gtaaatctcc
     1741 gaacatttcc tatcctaact ccgcgttctc cgcgtctctg cggttgaagt tcactttcga
     1801 ctcgtgctct ccgtgcctcg gtggtaagtc tccggaccta ttcacctatt ccacgtccgc
     1861 tgcgaacagc tcgttagggc tgaccacacc cggcgataaa ccttgttcgt aaacatattc
     1921 gccgatcgcc tgcagcgccg gccgattcgg ctccagcccg tacgcccaga aatctttacc
     1981 caagactcgc caggtctcct cgatgtgatg gatcaaccag ggaagcgaaa ggcgcagcgc
     2041 gtccgtgtcg tagagtccgt cgatcgccag gtcgcgagct ttgcagaagg ccttatagat
     2101 gctccgggcg gcccatgggt gctctcgata gatatcgtcg cgtagcacca ccgtatgcat
     2161 gatcgggaag attccggtgc gtcgataata gtcttgctcg acttcatagt agttcggaaa
     2221 caggcgcgct atccggggcg agcccttctc gaagatggag gggatatata tcccgagcaa
     2281 cgcgtccaga tccccgctct ccaacattcc ctccagcgtc cgctccaccg gcaaaaactc
     2341 gaccttgact ttgtccggca gcttcaacgg aatcaggggc cgctgcgttg gcgtcgtcaa
     2401 tccgccgatg aaccactgaa tatcctgcat cgcaataccg tattcgtgtt gcagcatgcc
     2461 gttcatgaag atggttgcgg tagatccgag ctgtgtcgtt cccacccgtt tgccccgcag
     2521 atcctgcggc gtccgaattc ctgcgtcggt gcgtacgtaa atacacgagt ggcgaaagat
     2581 cttggacagc gccaccggca cggcaacaaa ggggcaagcg ccgcggccta tcagactcac
     2641 ataagagcca agcgatagct ccgaaacctc gaactctttg tcctccagca ttcggggaaa
     2701 agtctcgcgc gggcgcatga ccttgatctc caagtcgatg ccttcggcct ggacacggcc
     2761 atcgataagg gggcgggtgc ggtcatagtc ccagcacgcc agggtaagtt tgagtttcga
     2821 catcggtgca acctgtctgc agagaaacct gctgtgaata tatcgtgaat ggtgtgcgta
     2881 gaaataacac accgcaagga gctgtcaaga aatccgttca tcgaagtcca atggttcgcg
     2941 cagcgcatag ctccggtctg cttggatccc accgcgatgg tgcggctttt agatcagatc
     3001 gttttgatcg tgcaattgcc gaggtgaaat catgagcgct taacgatctc gggttgcacg
     3061 agcgttgaac gttgacgttt gaacttttga atggctgttc gactattacg gtagcggact
     3121 ctccggcgtc agccgtttga tctggggtat gacctctctg ccgaagcgct cgatcgtttc
     3181 cagcgtaaag cgccggtcca tgcccggcca atccaccatg aaccacatca cttcggcgcc
     3241 gaactcttca tgataggcca tcacttgctc gaccacttcg gacgggctac cgagtatcag
     3301 gcggtcatac ttcaactcat cgaagttcag atcgtagcgc tcgcccggct gtccccattg
     3361 ctgatagcgc cggtaccgtc gttcgtaacc ttccttgatg cgctcttccg cttcgcgggt
     3421 cgtatcggcg atgcaaagat cacgaaaaat atacaagccg cgatatggct ttccctgccg
     3481 ttccaacgcc gccttatact ccggcaatgc ttggcgtaaa aaactcttcg agtggcgccg
     3541 gctggcaatc cagtggtcgg caacttccgg cactctggcg acggttctca ccgtatcggc
     3601 gcccatcaag atcggcggtc ccggccgctg caccggcgtg ggaaggctca tcacgttatc
     3661 gagatggaaa tgttttccat ggaaggtaac cggctctccc gtccagagct tgcgaatcac
     3721 gtccaatgct tcgaccaatc gacttctgcg ctgccgtaga tcgatgccaa atgaattgaa
     3781 ttcgttttct ctatagccct ggccgacacc gaagagaacc ttcccgccgg acagattatc
     3841 caacgtcgag atatattcgg cgaccatcac cgggtgatgc agcggcagaa gaaagatcga
     3901 agtgcccaga tacattcccg gtacctgcgc ggcaaggtaa gccagcaaag gaatcgactg
     3961 caggatcgcc gcatccggac cggtgagata atgttgtgcg acgaacaagg cctcgaaatt
     4021 gttctgatag gcggcctgcg cccgcagcga aacctgctcg cacacctcgg cggaatccaa
     4081 gtccgccgga taactgccga actcaatacc gaatcgcatt cctactccta ccaccaatca
     4141 acgatggacg tcggttaata tcatgaacgg gcttacggca gcggacatcg aattgctcga
     4201 gaagggttag cgccaggcaa tgaattccgg acagtcgacg atacctgtgt gagaagattt
     4261 ggatcaaggg ctctgatata cgagctttac ccgttcagaa ttgcgtgctt tgctaatttg
     4321 gtccgaggtc agtttatcgc ctgcgcgtga tgcttcgtac atcaagttgt aattgtttgc
     4381 gaccgacttg ggatcattac gcagatccgg gagactaagg agatggccga attcatgtgc
     4441 cagcgtcttc ccgagaactg gatcgccaag cgaggtgaca atgcagacac ccagcgcctc
     4501 tgcggcaaat cctcccagat ctctcttctc gaaacggttc acaaacaacg ccgccactgc
     4561 gccgatcggc ttgattttca aggccgtaat catctgaaaa aatccgctta cactgacctc
     4621 ctcgacattt ccgggtgcct cggttgctgg gaaaaagctc ttgcgcagct gaaaggtgat
     4681 atccgccgga gcccagatgg tgttggctac atcgatcgcg cgcataacgg ttttgtcgtt
     4741 ttgattagga ggaatgtcgc gggtttcttt gacgcgctca tgttgaatct gagtgctttt
     4801 accaaccgcg atcttctttt ccacgacctg gtagctctcg atctgaagcg tgatcaagta
     4861 gacatcgact ggtatggtga tggtcttcgc caagcattcc ctccatggac aggcggatac
     4921 taagggatat ggccgttaga gggcaagggg attttcttaa gaatgaagaa tagcactaaa
     4981 cccgtgatcg tgagcggaca agtggcggtg tcgcgatcct ataggaaaat agaaggaata
     5041 aaggaataaa aaaataaact tggtcatccg gaccgggtct gctcgccaaa gtctgatttt
     5101 cgtttcaagc gatgggcaaa atgattcttt aagctcggtt aacaacagcc gacctgcttg
     5161 acacgtagtt cgcgtttgcc ttatggtcgc ccgtaagtcg aaccgagaat tcacttcttt
     5221 ttcgaatttt atatccttag aaatcttctt accgtcacgg agggtgattg catatgaagc
     5281 ggctcgaaat tacgctggcc ttcatgactc tgtttatggg ttttgggtcg aacgccagcg
     5341 cgcaggattt ctacagcaac aagacgctgc gcatagtcgt cggattttca cctggcggcg
     5401 gaatggatat ctacgcgcgc gcaatcggac ggcatttggg aaaaaacatt cctggcaagc
     5461 caaccatgat cgttgagaac atgccgggcg ccggaagcct cattgcggcg aattatctct
     5521 acaatcaggc aaagcccgac gggctcacga ttggaagttg gatcggcgga ttggtcttac
     5581 aacaagtgat cggcggacaa aaggggatcg aatttgatgc gcgtaagttc gagtggatcg
     5641 gagcgcccgc aggagacagc tcgtcctgtg ccgtctcaca atcgagcggt atctccacca
     5701 ccgatcagtg gttggcagcg aagacgcccg tgaaaatagg cgcaatggca ccgggttctg
     5761 tgacttccga catacccaaa attctgaagg cggcgctaca tctgccaatt caaattgtcg
     5821 aaggatacaa aggaacggct gagatacgtg tggctgcgga ctccggagaa gttgatgggg
     5881 gttgctgggg atgggagact atcaaaataa tgtggaggca gggactagac tctggcagta
     5941 caaaagtcct catacaaatg ctccccaaaa gacatccgga ccttccggat gtgccgaacg
     6001 ctctcgagct cgcgaagaac gatgatgcgc gtcaattgat aaagataggc attgtcgatc
     6061 cggcaacaat ggttcgttct ttaaccctgc cacccggaac gcctaaggat cgagttaaga
     6121 tactccgtga cgcgtttatg gcgaccatga aagacccgca atttttagcc gaggcaaaga
     6181 cagccaagct cgacctgaat ccgctgccgg gcgatgaagt tgaaaagatc gtgcacggct
     6241 tcttcagcct gccgccgagt gttgcgacaa agctgcgcga catcgtcgtg tccaagaact
     6301 aactcacgta aaaggagaac gtaattatgc aaccgatgtt catccagcac gaaccgaaac
     6361 gggtgtactc aaaggcgtgc gtctgcggca attttatttt ccttgccggc gaagactgca
     6421 aagatcccaa aagccaggcc atccgcggga ccacggtgcc cgagcaattc gattacacgt
     6481 atcaaaatat gaaggagacc ttggaaagcc tgggcagctc gctcgaaaac atcgtcagcc
     6541 tgacgaccta cgtcaccgat ccgcgcgctc gccaagcgca ccagcgtgcc cgcgccaaat
     6601 ggctgccgca aaacccgcct agcgcgctca tctgcggcgt tcaactggcc gatccggaga
     6661 tgctggtcga gattcaaggc gtggcgatca tcccggaaaa aaaatagccc actcccttcg
     6721 ccgatcatcg ttctcttagc gacgacgatc ggcacacaaa acaagttgtc agcgttagga
     6781 tcggaggcgc gcatggacgc gttgcagcag cgcattataa acgaaatttc acaagacgag
     6841 ctcgtcgcga tgacggtcga tctggtgaat ttttatagcc ccaccggggg cgaagcggcc
     6901 atcgccgact acctcgccgg acgattgaag gaactcggca tgcgcgtgca actgcaggaa
     6961 gtcgaaccag ggcggaacaa tgtgattggc cgcctgcccg gcaaaatagg caacccgact
     7021 ctcctgttca gcggccactt cgataccagt accaccgggc gtgaagcgga ggcatggggc
     7081 ggagattaca gcgcggaaga cttcggcggc gggcagattc gcgctgcggt cacgaacggc
     7141 tggatcagcg gcgtcggcgc ttcgaacatg aagggagcgt ttgccgccta ttggtcggcc
     7201 ctgcgggcat tgcaacgcac aggcgtggag ctggtgggcg acgttctggt taccggtgtc
     7261 gtcggcgaaa cggaaaaagc gccggtcgat cagtataaag gtggagagtt ccgcggcggc
     7321 aaggcgggat cccgctatat ggtcacccat ggggtcactg ctgactacgc gatcatcggc
     7381 gagcccaccg gtatgcgcct gcagatcggc gagaccggct actgctttgc gaagatcacg
     7441 gtctatggca agtcccagca cacatggtgc aaggaattcg gcatcgatcc catcgaaaaa
     7501 atgatgcgag tgattgcagc cctcaaggct tgggagccga tctaccagag acgacatccg
     7561 cacgcgttca tggagccgcg cataggtatc ggcgccatcc aaggaggata tccgtacaaa
     7621 ccatccaagt gtcctgcgcc tttctgcaac ctctatgtcg atctgcgctt ggttccgggt
     7681 caaagcttca tggaaaccaa acgcgagctg gaagcggtca tcgcggatct aaaagccaac
     7741 gacccggatc tccgggccga ggttcacttt tacttaacgg gaaacgggta cgagctcgag
     7801 cgcgacagcg cagtcgtcat cgcgatggaa cgagcccacc gcgaagtgta tggggaggca
     7861 atatcgtacg cagcgcctag tcgatatgct gtttccagtg acgccggccc gatgttcgag
     7921 tatgggatca aggggttgac ctatggaccc ggtggtgtct ccgcggccgg ttcatttacc
     7981 gtgtacgatc ccagccaaca gcaaagtgaa gtgcttagcg tagaaaacct tgtcaaagca
     8041 gcgagagtgt atgcgctcgc tgcactcgac ctgtgcggat ctaatttgaa cacgcaatga
     8101 acacatcggc agcgcgggtc ggacgagcca ggttgatata gtttctgccg tttcatgcag
     8161 aactaggtct tagaacctcg tttccg
//
LOCUS       DASUVS010000257         2427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_323826, whole genome shotgun sequence.
ACCESSION   DASUVS010000257 DASUVS010000000
VERSION     DASUVS010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323826"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VF182_14185"
     CDS             <1..671
                     /locus_tag="VF182_14185"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6438284.1"
                     /translation="NRDAVKQYEGPNGFEIPGEVVPGGRIVATDKSPQSVDASRARVT
                     NEKLSDVINVRVGDATALEFSDQTFDFVVAVQVYSFVANITRAIEEAARVLRKGGRLL
                     VLESDWDMCLWKSKDLRFTRRMISARAETQFVHAHLPRDLHKYLRFSGLTLADVQTFS
                     IVETHYDPESYGASVIGITRDSALKHGMPAEAVAAWEQDLRSRTAEGEWFFCLDRFVF
                     TAIK"
     gene            749..1108
                     /locus_tag="VF182_14190"
     CDS             749..1108
                     /locus_tag="VF182_14190"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6438285.1"
                     /translation="MTKIRHIAYRADDVDAMAKFFIDAFGLTMVQKRKDNSIDLSDGS
                     INISILPLAANRSRRAGLDHIGFSAEDNWEQMRRVEAAGARKERTRSSYEEKFKGPEG
                     IIVDVGRWPGAAPAAKE"
     gene            1316..2107
                     /locus_tag="VF182_14195"
     CDS             1316..2107
                     /locus_tag="VF182_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6438286.1"
                     /translation="MSSNRFDLSGRVALVIGASRGIGRAIAKGLAEAGASVALCSRKE
                     TDLLKVAEEIRALGAGAEIFAADVSRVAEIQTLVRDVLGRLGQIDILANVAGINCRKP
                     STEITEEDWDSVLALNLRALFFSSQAVGRYWIETRRFAPDNKRGKGKIINIGSIAGEM
                     GIRHVAPYAASKRGVVGVTNTLAVEWACAGICVNCLAPGYIETDLTRARFDDPAFTTA
                     FHNRVPMRRRGFPADVVGAAVFLAAPASDYLTGQTIVVDGGFLIA"
BASE COUNT          538 a          618 c          707 g          564 t
ORIGIN      
        1 gaaatcgcga tgcagttaaa cagtatgaag ggccgaacgg cttcgagatt cccggtgaag
       61 tcgtcccggg aggccgcatc gttgcgaccg acaaaagtcc gcagtccgtt gatgcttcaa
      121 gagctcgggt tactaatgaa aagctcagcg atgtgattaa cgtccgggtt ggcgatgcaa
      181 ccgcgcttga gttttctgac caaactttcg attttgtagt cgctgtccag gtgtactctt
      241 ttgttgcgaa catcactcgg gccatcgagg aagcggcgcg ggtgttacgc aaaggcggcc
      301 ggctgctggt gctcgaatcg gactgggaca tgtgcctgtg gaaatccaaa gatctgcgtt
      361 tcactcgccg catgatttca gcccgtgccg agactcaatt cgtccacgct cacctgccgc
      421 gcgatttaca caaatatttg cgcttttcgg gcttgacgct tgccgatgtg caaacattct
      481 caatcgtcga aacgcattat gatcctgagt cgtatggagc cagcgtgatc ggcattactc
      541 gcgattcagc gctcaagcat ggtatgccgg ccgaagccgt cgccgcctgg gaacaagatc
      601 tacgctcacg tacggctgaa ggtgaatggt tcttttgttt ggatcgcttt gttttcacgg
      661 cgatcaagta aggctcaatc attcgccgcg tggatagcac gctgggcgaa gtggataagt
      721 ccgattattg aatcgacgag gtttggcgat gactaagata aggcatatcg cctaccgcgc
      781 tgatgacgtc gatgcgatgg caaagttttt tatcgatgcc ttcggtttga cgatggttca
      841 gaagcggaag gacaactcca tcgatctgag tgacggttca attaacatat cgattctacc
      901 actcgccgcg aacaggagcc gtagagccgg gctcgaccat atcggctttt ccgccgagga
      961 caattgggag cagatgcgca gggtggaggc agccggcgcg cggaaagagc ggacgcgatc
     1021 atcctacgaa gaaaagttta aaggtcccga agggatcatc gtcgatgtcg gccgctggcc
     1081 aggcgcggcg ccagcggcca aagagtaatg gggaacgact tctcagatcg gtggcgctgc
     1141 aatgacttct cagtcattcg cgttcctact gccggttagg caaacgaact ttcgctggaa
     1201 cctaaggaag aagttcgtac ggcattgccg acgacattga tggcgtagtc aaattccagg
     1261 gaccctgcaa gcatgcccta gatatcacta aagagcgggg ttggggtaag cgataatgtc
     1321 ctctaatcgg tttgatctct cggggcgggt ggcgctcgtt atcggcgcat ctcgcgggat
     1381 cgggcgcgcg attgctaagg gcctcgctga agcgggcgca agtgtcgccc tctgcagtcg
     1441 caaggaaacg gatcttctta aggttgcaga agagattcgt gccttgggcg cgggtgcaga
     1501 gatttttgcc gcggatgttt cgcgcgtggc ggagatccag acactggtgc gcgatgttct
     1561 cgggcgcctg ggacagatag atatactcgc aaatgtcgcg ggcataaact gtcgcaagcc
     1621 gtctacagag attaccgaag aagattggga cagcgtgctc gctctcaatc tgagagcctt
     1681 gttcttctcg tcacaggccg tcggaagata ttggatcgag actcgccgct ttgctcctga
     1741 taataagcga ggtaaaggca agatcatcaa tatcggcagt atcgccggcg agatgggtat
     1801 tcgccatgtg gctccgtatg ccgcgagcaa acggggcgtg gtgggagtga cgaacaccct
     1861 ggccgtcgaa tgggcgtgcg caggcatctg cgtcaactgt ctcgcccctg gatacattga
     1921 aaccgacctg accagagcaa ggttcgatga tcccgccttc acgactgcat ttcacaatcg
     1981 ggttccgatg cgccggcgtg gtttcccagc cgacgttgtc ggtgctgctg tttttcttgc
     2041 agcgcctgca agtgattacc tcaccgggca aactatagtc gtagacggcg gctttctgat
     2101 cgcgtaatcc ttacgtcacc ccccgtaaga agtaactttt ttcttactgt ccggtgatgc
     2161 cgacgtaatg taaaggttac ccgtctcgat ctctttgctc gtaccgcgca actctgtgaa
     2221 ccaatttccc atggaaaatc cagtgcgttc atcgaaccgc ttccggttga actatgttgg
     2281 cgtactcgtt gctctccatt ccgttaccga atgcaaggag gacaaggcca tgagtaaaaa
     2341 aatattggtg ccactggggc ggaatgaccg gaccgaagag atgattcctt acatcgagaa
     2401 ggtcgctcga ccgggaatga acgtcgt
//
LOCUS       DASUVS010000258        12961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_542852, whole genome shotgun sequence.
ACCESSION   DASUVS010000258 DASUVS010000000
VERSION     DASUVS010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12961
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542852"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VF182_14200"
     CDS             <1..542
                     /locus_tag="VF182_14200"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6438287.1"
                     /translation="SYADLLKPEWKDNIGMDSKAYEWFGTMLKSMGEEKGLAYMRELA
                     KQTQLRAGRTLIAQLVAAGEFKGALTAYSQTFEVLKPSGAPVDWVYLNPVFANIHPAG
                     ISAKAPHPSAARLFIDYVLSKPGQEIVRKMNRIPDRTDTPPAQARLMEGIKPAFAPVE
                     VLDDFSRYGKMFDGIFAAR"
     gene            606..1382
                     /locus_tag="VF182_14205"
     CDS             606..1382
                     /locus_tag="VF182_14205"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6438288.1"
                     /translation="MKVVVRFLTKEFTSGSGTVRALGGVDFEVRDREFFGIIGPTGCG
                     KTTLLHIIAGLEKPTTGSVEFVGEKRTKSTVSMVFQESALMPWRSVEENVPLGAEFRN
                     EHPAILKRVSQFFLEVVRLLDFGKARPYELSGGMKQKVAIARALANDPEVILMDEPFA
                     SLDAQTRLLMREELLRIWERDKKTVILVTHNLDEAVMLCDRIAVMSSAPGLIKSIITV
                     DVPRPRNFRSMKDPDFARCTEKIWDLLRYDVDQAMRQNSV"
     gene            1478..2941
                     /locus_tag="VF182_14210"
     CDS             1478..2941
                     /locus_tag="VF182_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438289.1"
                     /translation="MIRVPSKETICRLTIWTLVYLIPAYQAMLPIEDPDIWWHLRTGQ
                     WIVAHGHVPIEDPFSMFGAGKPWIAYSWLFEVLVYGLYRNFGLTGLVAFTVTMSLVIG
                     LTIHVLVRRARLPFVAEVLLLAVIFASLKPLLSPRPWLFTILFTGIELLLISSLRRSW
                     KLLPALLLSLVFVLWANLHIQFVYGLAIPAFLTVESMVHRFAKSGLPQTDSKALSAMH
                     SLLLLGSCLAATLVNPYHYRVYLAILEYAMQSGAFQNISELHPMFFRSLADWLVLSLT
                     LTGAFVLGWQRRWNVFYLALFVTAACFAFRARRDVWFMALVVAAIVGEFARTTMSSEY
                     FDFTKERIAAVILSLLLSLYGLSRFRAVSEDSLARAVELHFPAQAVKFIRSNQLAGPI
                     YNSLDWGGYLIWSLPEFPVAMDGRTNLHGDERTARSLATWNGSIAWDKDPELLRARLI
                     IAQKGKPLTHLIRDDSRYRLVYEDDTAAVFVVDGSML"
     gene            3066..3794
                     /locus_tag="VF182_14215"
     CDS             3066..3794
                     /locus_tag="VF182_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX6438290.1"
                     /translation="MSKVFSQRVILLLGFVSMVAAGGCATTRSAQDFSSILADKERPD
                     TERALDSTRKPNEVLAFYGVKSGDKVADIYSARGYYTAILSGVVGPQGTVYSVNPAAR
                     PELAERVKSPKFANVRLVDGPLEKLALPQDGSLDFVLTHLNYHDLSPDVRTAMNKRIL
                     GALKRGGVYGVVDHSAKEGSGNEAVKTLHRIDKQLVIKEVTGSGFKLAKEGTMLSRPE
                     DKRDFNVNKERNLSDRFVLAFEKP"
     gene            complement(3952..4890)
                     /locus_tag="VF182_14220"
     CDS             complement(3952..4890)
                     /locus_tag="VF182_14220"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6438291.1"
                     /translation="MALLIGEPQTKKLINIARAVDLLEKMFLDRAAGKVRGLPRRRLK
                     GNEKQINIMAAWHQGYDLFCLRAYIAQANTVTLYNGKSGQIQAIINMGYLSSLRTGAA
                     TGVAAKNLAPADARTLGVIGPGRQATFQVEAVAQACQIERIVVFGRTPKRRKQFIKQM
                     SQIVQAEWREASSVEEVESESDILVVSTNSTTPVADGKNLKREVLVASIGANGAVKHE
                     VSGNLIRQMDLIVVDDVAAARTDSGDLIDACQSGIARWDDIVPLETLVGSGAPQPRPK
                     RILFQSNGIADEDLAVGRYVLEQAKRKLKLRQVTEI"
     gene            5209..6084
                     /locus_tag="VF182_14225"
     CDS             5209..6084
                     /locus_tag="VF182_14225"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX6438292.1"
                     /translation="MFSMKRCPNCSTEYDDKISFCTKDGRALVTADTSRARLCPHCAN
                     SIEENAVKCPYCKADFGTTAERPQWPGRDEIAGQSTRSAGKPASRSLTKPVIAILAAS
                     LMVVLAVGAFFFAGYRQRNDARQLEEAKLKQVQEREEKIKTLEGELSHLRQELDKSKS
                     QLTSLTAKLEENQKELSSTQQRLTAATRETERAAAKRPTVAPASSARSVKTPPSQTPA
                     PRRSAEPGVYEVIRPTTVREEPSTTGRRVTEIDKGTKITVVRTVGNWLEVRSKQGRPP
                     GYVRLDDAVLVTRAN"
     gene            6206..7186
                     /locus_tag="VF182_14230"
     CDS             6206..7186
                     /locus_tag="VF182_14230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6438293.1"
                     /translation="MQLCGFFFALFLLIPDQVWSQGIRGARGAGRGFSGFVNAASTSP
                     VRPRLGALDGSLTGHRDIFARSRSGAAHFRRRSFGHFTLPGSIVVQRFYPPFIDYQRY
                     PYVPYLDGYGYFPYYPGPSIIEIPNSSASIATNSMAPGLPPENFAADPLSERRFVRTL
                     PDQLAPFDPTPHEVVDRMLALSGIKAGDVLYDLGSGDGRILITAAKKYRIKAVGFEVD
                     TGLVKLAREKIRQENLEQLAEARHQDFMTADLSSASIVTLYLSHDGNQALKPMLLRQL
                     QPGARVVSYTFDMGDWPPNITESYRDGAGNVHALYYWEVPQSQVYSDKAQ"
     gene            complement(7261..8106)
                     /locus_tag="VF182_14235"
     CDS             complement(7261..8106)
                     /locus_tag="VF182_14235"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX6438294.1"
                     /translation="MEKQYTRETLLISAQALKEKLGSDSLCLIDVRAAEDFAQGHLPG
                     AVHFDLFGLSLVDTHEAPLRAFMHMIHHVFELRGVSETKEVIVYENNSGMRAARALWF
                     LDYFGHPNARMLDGGMQAWKAAGFVMTTEVTSPKGAGFKISERREVLATVDDVLQSLD
                     KKDISILDTRSRAEYVGRQIRAARGGAIPGAIHIEWTDNLDASGKFKPDAELKAMYSR
                     AGITTDKEVISYCQGGYRAAHSYIALRLIGFPKIRNYIGSWKEWGDRADLPVEQPDPE
                     VNASS"
     gene            8156..9295
                     /locus_tag="VF182_14240"
     CDS             8156..9295
                     /locus_tag="VF182_14240"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEX6438295.1"
                     /translation="MKNKRILNRLIFLILLGVFSLSPSAKGLGQTENSASSFSYRLAL
                     PGRTLSFPADHYSHPDFKTEWWYYTGHLQAKSGQLFGYQVTFFRFGVRDRQTNIKEKP
                     PLFSELYMAHFALSNISDRSFLFRERINRGIDDRAGAAIDRFFVWNEDWSVKGEGNNH
                     IISVNDRGTELRLKLKSLKPPVLHGQNGYSQKGEGEGHASYYYSLTRMQAEGELIIGG
                     KREQVHGLSWMDHEFGSNQLGEDQLGWDWFSIQLENQSEIMLYLMRRKDGSIDRYSHG
                     TLVYADGTTRQLALKDYRIDVLARWQSPKSAADYPIKWKVLVPAEQIELEISPHFAEQ
                     ELITNRSTRVTYWEGAVQVKGMLRNKPIAGSGYVEMTGYAGKVKF"
     gene            complement(9419..9655)
                     /locus_tag="VF182_14245"
     CDS             complement(9419..9655)
                     /locus_tag="VF182_14245"
                     /inference="COORDINATES: protein motif:HMM:NF022229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX6438296.1"
                     /translation="METWRPEEIGATAGKVWNFLHKRGESSLSAVERGVGAPKQLVCM
                     AIGWLAREGKLALRQEKRSLQLCLTDGEQNSRVQ"
     gene            9903..11651
                     /locus_tag="VF182_14250"
     CDS             9903..11651
                     /locus_tag="VF182_14250"
                     /inference="COORDINATES: protein motif:HMM:NF006203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX6438297.1"
                     /translation="MSNDKVKSVPVSTGAEAFLAQVRALRAVRYLFANTGTDHGPIIE
                     ALAKTATEDPNDLQPIVVPHELAAVSMAHGYYNVTQKPQMVLVHTLPGTANALGGIIN
                     AQSANVPVLLVAGRTPITEGELRGGKSQNIHWRQESRDQGNIVREFVKWDYEVRTNQN
                     LAAVVSRAFKIAMSEPRGPVYLTLPREWLCEELESTDLSSDSLTASTKMQAEYAALEQ
                     AAELLIRAEHPLIVTKYLGRNPEAVPLLVELAELLAIPVVQQMSYLNFPTDHPLYLGV
                     QTIQHSKEADVLFFIDTDVPWEPPSRRFIRSDVKIIHLERDPLFTGIPGWGFPADWPI
                     TGCSEVSLPTLTSIVRSKLQKSKTKQNRFEERRKRIEAEHHAIWRQVEADMDAVKNEK
                     PISPLWVSKCIGDVVDEKTIIVNETVTSKLADVIHLNRPGSQFGTPLAGHLGWGLGAA
                     IGIKLGSPEATVIAAEGDGSYMFCAPTACHFTAQKYRIPFLTVVYNNQAWNASLNAAR
                     GLFPDGFAQKSGNFPGTDLSPSPKFELTAQACGAYAERVEEPEAIPDALKRALKVVRE
                     EKRQAMLNIICKDPLA"
     gene            12252..12578
                     /locus_tag="VF182_14255"
     CDS             12252..12578
                     /locus_tag="VF182_14255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX6438298.1"
                     /translation="MKKKMLRGQASWYGPGFHGKKTASGEIFDQRRLTAAHKTLPLGT
                     IAKVTNLENGNTVEVEINDRGPYVGERVIDVSRAAAKALGFIDSGITLVRIEPLYQES
                     GTEEAG"
     gene            12652..12819
                     /locus_tag="VF182_14260"
     CDS             12652..12819
                     /locus_tag="VF182_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX6438299.1"
                     /translation="MLSWSITFLVIGLIAGLLGLSGIAGAATQIAWVLFVVFLVLFLV
                     SLVMGRRSPPV"
BASE COUNT         2949 a         3371 c         3526 g         3115 t
ORIGIN      
        1 agtcctatgc cgatctgtta aagccggagt ggaaggataa tatcggcatg gatagcaaag
       61 cctacgagtg gttcggcacc atgctcaaat ccatgggcga ggaaaagggt ttagcctaca
      121 tgcgtgaact ggccaagcaa acgcagttgc gcgccggccg cacgctaatt gcgcaactcg
      181 tggcagctgg agagttcaaa ggggcgctga cggcatactc gcagaccttc gaagtgttaa
      241 agcctagcgg tgctccggtg gactgggttt acttaaatcc ggtttttgcc aatattcacc
      301 cggccggtat ttccgcaaaa gcaccgcatc ctagtgcggc gcggctgttc atcgattatg
      361 tcttatctaa accgggccaa gaaatagtgc gcaaaatgaa caggattccc gatcgtacgg
      421 atacgccgcc ggcacaagcg cgcctgatgg aaggcatcaa gccggctttc gcaccggttg
      481 aagtcctgga tgatttctcg aggtacggca agatgttcga cggcatcttc gctgcccgct
      541 aacgccttga gaaggaacgt ttaagattca acgttttacc gtttcacttc ggcgataggt
      601 cgtcgatgaa agttgttgtc cgttttttaa ccaaagagtt tacatccgga tcgggaaccg
      661 tccgcgccct gggcggcgtc gatttcgagg tgcgcgaccg cgagtttttc gggatcatcg
      721 ggcccaccgg ctgtggcaaa accacgctat tgcatatcat cgcgggcctc gagaaaccga
      781 caaccggatc cgttgaattt gtcggcgaga aaagaaccaa atcgacagtc tcgatggttt
      841 ttcaggaatc ggctctgatg ccgtggcgga gcgtcgaaga gaacgtcccg ctcggagcgg
      901 agtttcgcaa tgagcacccc gcgatattga aacgggttag ccagttcttc ctggaggtag
      961 ttcgtctgct ggacttcggc aaggctcgac cctacgagct ctccggggga atgaaacaga
     1021 aagtcgccat cgctcgggcc ctcgccaatg atcctgaagt gattctcatg gatgagccgt
     1081 tcgcctcact cgatgctcag acccggctgt tgatgcggga agagctcctg cgcatctggg
     1141 agcgcgacaa gaaaaccgtc attctcgtga cgcataatct cgacgaagcg gtcatgctct
     1201 gcgatcgtat cgcggtcatg agctcggccc ccggcttgat caagagcatc atcaccgttg
     1261 atgtgccgcg gccgcgcaac tttcgcagca tgaaggatcc cgatttcgcc agatgcacgg
     1321 aaaagatctg ggatctcctg cgctatgatg tcgatcaagc gatgcggcaa aattctgttt
     1381 aatgcttctt cctcgcatat cggaatacag cccacccatt caaatccgta tccctgatcc
     1441 ccgccttaga cgctagacca aagaacgatt acaacgaatg ataagagttc cgagcaagga
     1501 aacgatttgt cgtttaacca tatggactct ggtgtacttg attcccgcct accaggcgat
     1561 gctgccaatc gaagaccccg atatctggtg gcatttaaga accggccaat ggatcgttgc
     1621 tcacggccat gtcccgatcg aagacccgtt ctcgatgttt ggcgcaggca agccgtggat
     1681 tgcctatagc tggctgttcg aggtcctagt ttacggtcta taccgaaatt ttggtttgac
     1741 cgggttggtt gcgtttaccg tgacgatgtc cctcgttatc gggttgacga tccatgtgct
     1801 cgttcgccga gctcgcttgc cttttgtcgc tgaagtcctc ttgctggcag tgatttttgc
     1861 cagcctaaag cctctgctaa gtccgcgccc atggctcttc acaatcttat tcactggcat
     1921 agagctgctg cttatctcca gtctgcggcg ctcatggaag cttcttcccg ccttactttt
     1981 gtctctggtg tttgtcctct gggccaattt gcacattcag tttgtctatg gattagctat
     2041 cccggcgttt ctgaccgtcg aatcgatggt ccatcgattc gctaaatccg gtctgccgca
     2101 aaccgatagc aaggctctgt cggcgatgca ttctttgctg cttctcggtt catgtttggc
     2161 tgcaacgctg gtcaatcctt atcactaccg tgtttacctg gcgattctgg aatatgctat
     2221 gcagagcgga gctttccaga atatctctga gctccatccc atgttcttcc gttcgctagc
     2281 cgattggttg gtgctttctt taactctaac cggggcgttt gtactcggtt ggcagagacg
     2341 ctggaacgtt ttctatttgg ctttgttcgt gacggcggct tgttttgcgt ttcgcgcgcg
     2401 ccgcgatgta tggttcatgg ctttggtcgt ggcggctatc gttggcgagt tcgctcgtac
     2461 gacaatgtcg agtgaatatt ttgatttcac caaggaaaga atagcggccg ttattcttag
     2521 tttactgctc tctttgtacg ggctgagccg ttttcgtgct gtttcagaag attcgttggc
     2581 cagagccgtc gaactgcact ttccggccca ggcagtgaag tttatcagaa gtaatcagct
     2641 tgcagggcca atttacaatt ctctcgattg gggaggttat ctgatttgga gtttgcccga
     2701 gtttccggtt gcgatggacg gccgcacgaa cttacacggt gatgaacgga ccgcccgttc
     2761 actggcgacg tggaatggga gtatcgcctg ggacaaagac cctgaacttt tgcgagcccg
     2821 tttgatcata gcgcaaaagg gcaaaccgct gacccatctg attcgcgacg attcccgtta
     2881 tcggttggtt tatgaagacg acacggctgc ggtgtttgtg gttgatggga gtatgttata
     2941 actcatacgg caagattagg cgtgccgacc aagattttcc ttgtgcagca taggcgtcaa
     3001 cccctttgat gtacatcgaa caatcgcggt taatgatgag gcgatatttt tagcaagggg
     3061 gagttatgtc caaggtgttc tcacagcgag ttattttact tctgggattt gtatcaatgg
     3121 ttgccgccgg cggttgcgct actacgcggt cggcgcagga tttttcatct atcctggccg
     3181 ataaagaaag gccggacacc gaaagggctt tggattcgac ccgcaagcca aacgaggtgc
     3241 tcgcgtttta tggcgtgaaa agcggcgata aggttgccga tatctattcc gcgcgcggct
     3301 attacacggc aatcctgtct ggagtggtgg ggcctcaggg aaccgtctat tctgtgaatc
     3361 ctgccgcgcg gccggaactg gccgaacggg taaagagtcc aaagtttgcc aatgtccggc
     3421 tggttgacgg gcctttggaa aaactcgcct tgccgcaaga cggttccctc gacttcgttt
     3481 tgacacattt gaattatcac gacctctccc ccgatgtgcg gaccgcgatg aacaaaagga
     3541 ttctgggggc tctgaagcgg ggcggtgttt atggtgtggt cgatcactca gcgaaggaag
     3601 gcagcgggaa cgaggcggtt aaaacccttc atcgcatcga taaacagtta gtcatcaaag
     3661 aagtgactgg ttcagggttc aagctggcaa aagaaggaac gatgcttagc cggccggaag
     3721 ataaacggga cttcaacgtg aacaaagagc gaaatctgag cgaccgcttc gtgctggcgt
     3781 tcgagaaacc gtagagcgaa tacaagatcg tttgcgatta tcatttaaac tagctgagct
     3841 aaatctagct gagccgccga tcgagaccgg ggcggcggct cagaacctga cccaagtaag
     3901 tcgcaattcg cctccacgct aacagcgccg ggactctgca gcgaattaag cctatatctc
     3961 tgtgacttgg cgaagcttca gttttcgctt cgcctgctcc agcacatagc gccctaccgc
     4021 gagatcctca tcagcgatcc cgttggattg gaaaagaatt ctcttgggcc tgggttgcgg
     4081 cgcgccgctg ccgacaagtg tttccagggg aacgatatcg tcccaacggg cgatgccgga
     4141 ctgacaagcg tcgatcaaat caccgctgtc cgttctcgcg gcggccacat cgtcgaccac
     4201 gatgagatcc atttggcgaa tcaagttgcc cgaaacttcg tgtttgacgg ctccgtttgc
     4261 gccgatcgat gcaaccagca cctcccgttt gagatttttt ccatcggcaa cgggagtggt
     4321 cgagttggtc gagaccacta ggatgtccga ctcgctttca acctcttcaa cagaggatgc
     4381 ctcgcgccac tcggcttgga cgatttggct catctgttta atgaattgtt tgcgccgctt
     4441 gggagtcctt ccgaagacca cgatccgttc gatctgacac gcctgtgcaa ctgcttccac
     4501 ttgaaaggtg gcttgccgcc ctggcccgat gacccccagg gtcctagcat cggccggcgc
     4561 gaggtttttt gcggcgacgc cggtggcggc gccagttcgc aggctgctaa ggtagcccat
     4621 attgatgatg gcctgaatct gcccgctctt gccgttatag agcgtgaccg tgttcgcttg
     4681 cgcgatgtag gcgcgcaggc aaaacagatc gtaaccctga tgccatgcag ccatgatgtt
     4741 gatttgcttc tcgttcccct tgagtcgtcg gcgcggtaaa ccgcgtacct tgccggcggc
     4801 ccgatctaaa aacattttct cgagaagatc gactgcccgg gcgatgttga tgagcttttt
     4861 ggtttgtggt tcaccgatga gtaaagccat gttcgttgtc gtcctccatg cgttctatca
     4921 ggctgcttaa agcctcgtat cccttgggaa aggatgaatt atgaaagctg aagattaaat
     4981 ttcgctttca gatttccgct ttcatccttg taatttgcgc gctttgcctg tgaaatcttt
     5041 gagcagcctg tacccagagt ttgtccccgt tctatagcgc agagcggata tgccggcaac
     5101 aaacaatggg ctatttctta tcacgtcata gagcaaggac taatgcttga gccgggtagc
     5161 aggtgactcg catgctttcc cttgacccgt gagctgattc atttaacaat gtttagcatg
     5221 aaacgctgtc ccaattgttc aaccgagtat gatgataaaa tcagcttctg taccaaggac
     5281 ggcagagcgc tggtaaccgc cgacacgagc cgtgcgcggc tttgcccgca ctgcgcaaac
     5341 agcattgagg aaaatgctgt aaaatgtcct tactgcaagg cagacttcgg aaccaccgcg
     5401 gagagacctc aatggcccgg tcgggatgag atcgcgggcc aatccactcg tagcgccggc
     5461 aagcctgcca gccggagttt gacgaaaccg gtgatcgcga tactggcggc gagcttgatg
     5521 gttgtccttg cggtaggtgc atttttcttt gccggttacc ggcagagaaa cgacgctcgc
     5581 cagttggaag aggccaaact caagcaggtg caagaacgcg aggaaaaaat caagacactc
     5641 gaaggagaac tgagtcacct tcggcaggaa ctcgataaaa gcaaaagcca gctgacgtcg
     5701 cttacggcaa agctcgaaga gaaccaaaag gaactttcgt caacccagca gcgattaacc
     5761 gccgcgacac gggagacaga gcgcgcagcg gcaaagcgcc cgaccgtagc gccagcatca
     5821 tcggcgcgtt cggttaaaac gccgccctcg caaacgccag cgccgcgccg ttctgctgag
     5881 cccggggttt atgaggtcat ccgtcctacg accgtgcgtg aagagccctc gaccacgggc
     5941 cgcagggtga cggagatcga taagggtacg aagatcacgg tggtacgcac tgtaggaaac
     6001 tggcttgaag tccgatccaa acagggcaga ccgccagggt atgttcgttt ggatgatgcg
     6061 gtactcgtta ctcgagcgaa ctagaaccag cacgcttcca tatcctaatc taaagggccg
     6121 gtctcagaaa ttctgtttag tggctaaccg ccgctattga tgctagttga cttatgctca
     6181 tgagtgctgc ttaagattta gcaggatgca gctttgcgga ttttttttcg ccttattctt
     6241 attgattccg gatcaggttt ggagccaggg aattcgcggc gctcggggcg ctggccgcgg
     6301 cttcagcggt tttgttaacg ccgcatccac ctcgcccgta agaccgagat tgggcgcact
     6361 cgatggttcc ctgacgggcc atcgggatat ttttgcgcgt tcgcgcagcg gtgccgctca
     6421 cttccggcgt cgatcgtttg gccatttcac cctgccggga tcaatagttg tccaacgttt
     6481 ttatccgccg tttatcgatt atcaacggta tccgtatgta ccgtatttgg acggatatgg
     6541 ttacttcccg tattaccctg gaccgtccat catcgagatt ccgaactcat ctgcttcaat
     6601 agcgactaac tcgatggcgc ctggtctccc tccggagaat ttcgccgccg acccattatc
     6661 ggagcgacgt ttcgtaagaa cgcttcccga tcaactggct cctttcgatc ccacgccgca
     6721 cgaggttgtg gaccggatgc ttgcgcttag cggaataaaa gctggagatg ttctgtacga
     6781 tctcggctct ggcgacggtc ggatcctcat caccgcagca aagaaatatc gaatcaaagc
     6841 agtggggttt gaagtggata ccggattggt aaaactggcc cgtgaaaaga tcaggcagga
     6901 aaatctagag caactcgcgg aagcgcgcca tcaggatttc atgacggccg atttatcctc
     6961 agccagcata gtgacattgt atctttccca tgatggcaac caagcgctca agcctatgct
     7021 actgcgccaa cttcagcccg gcgcgcgggt agtttcatac acctttgaca tgggcgactg
     7081 gccgccgaac attaccgagt cctaccgtga tggagcgggc aacgtgcacg cgttgtatta
     7141 ttgggaagta ccgcaatctc aggtctatag tgataaggcc cagtaatcgg ccctgacgta
     7201 tttacggttc gtcggtatgt cgggtcggca tcgcagccga tcaaataaag acatccgcac
     7261 ttagctgctt gcattcactt ccgggtccgg ttgctcgacg ggcagatcgg cgcggtcgcc
     7321 ccactctttc catgagccga tgtagttacg gattttagga aagccgatta agcgcagcgc
     7381 gatgtaacta tgcgcggccc ggtaccctcc ctggcagtag gagatcactt ctttgtccgt
     7441 cgtgatgccg gcacggctgt acatggcttt aagctcggcg tcaggtttga atttgccgct
     7501 ggcgtccagg ttatcggtcc attcgatatg aatcgcgccg ggaatggctc cgccccgggc
     7561 cgctcgaatt tgcctgccga catattcggc gcggctccgc gtgtcgagga tcgagatgtc
     7621 ttttttgtcg agcgattgaa gaacatcatc tacggttgct agcacttcgc ggcgctcgct
     7681 tattttgaaa ccggcacctt tgggcgaagt aacctccgtg gtcatcacaa atccggccgc
     7741 tttccacgcc tgcatgccgc catctagcat gcgcgcattg ggatggccga agtagtctaa
     7801 gaaccacaac gcccgcgcgg cgcgcatacc cgagttattc tcatacacga tcacttcttt
     7861 ggtttcgcta accccgcgca gttcgaatac gtgatggatc atgtgcataa aagctctcag
     7921 cggcgcctca tgggtatcga cgagactgag cccaaacaaa tcaaaatgga ccgcgccagg
     7981 taggtggcct tgagcaaaat cctcggccgc tcgcacatcg atcagacaga gactgtcaga
     8041 gcccaatttc tccttaagcg cctgggccga aatcaaaagc gtttctcgtg tgtattgttt
     8101 ttccatagtg tccctatagc attagtcgga aagaatccgc aattgtgaat cgaatatgaa
     8161 aaacaagaga attctcaacc gattaatctt tctaattctg ctcggagttt ttagcctgtc
     8221 tccttcggca aaagggctgg gtcaaactga aaacagcgct tctagtttca gctatcgact
     8281 cgccttaccc ggcagaactc tgtcatttcc cgccgaccac tattcccatc ctgacttcaa
     8341 aaccgaatgg tggtactaca ccggccatct tcaagcgaaa agcggacagc tcttcggtta
     8401 tcaggtgaca ttcttccgat tcggcgtgcg ggatcgtcag accaatatca aagaaaagcc
     8461 gccgctgttt agcgaattgt acatggcgca tttcgccctt tcgaacatct ccgacaggag
     8521 ttttctgttt cgggagcgta tcaaccgggg cattgacgac agggctggcg ccgctatcga
     8581 ccgcttcttt gtctggaacg aagactggtc ggtcaaaggc gagggaaaca accatataat
     8641 ttcggtgaat gaccgcggca cggaactccg gcttaagttg aagtccctca agccaccggt
     8701 gctgcatggt cagaatgggt acagccaaaa aggcgaagga gaaggtcacg catcttatta
     8761 ttattccttg acacgaatgc aggcagaagg agaactgatc atcggcggca aacgggaaca
     8821 ggttcacggt ttgagctgga tggatcacga attcggcagc aaccaactcg gcgaggatca
     8881 gctcggctgg gattggttca gtatccaact cgaaaatcaa agcgagatca tgctttatct
     8941 catgcggcgg aaggatggat ccatcgatcg gtactcacat ggaacgcttg tttatgctga
     9001 cggaacaacc cggcagctgg ccctgaagga ctatcgaata gatgtcctcg cgcgttggca
     9061 gagtccgaag agcgctgctg attatcctat aaagtggaaa gtgcttgttc ctgccgagca
     9121 aatcgagtta gaaatctctc ctcattttgc cgagcaagaa ctgatcacga atagaagcac
     9181 gcgtgttact tactgggagg gtgcggtgca agttaaaggg atgctccgaa acaaacccat
     9241 tgccggatcg ggttacgtcg aaatgaccgg atatgcaggc aaggtaaagt tttaagcgtc
     9301 gaaggattgt ctcggcggcc tgccgaagtc tcaactccaa cttctttttt gtttaattgc
     9361 gaaattgccc ctgcgtgcgc atctccgggc atatgcatga tgacactctg ccgctctgtt
     9421 actgcactcg ggaattttgc tcgccgtcgg tcagacacag ttgcaacgag cgtttttcct
     9481 ggcgtaaggc aagttttcct tcacgtgcaa gccaaccaat ggccatacat accagctgtt
     9541 tcggggcgcc cacgcctcgt tctaccgcgc tgaggcttga ttcaccgcgc ttatgcaaaa
     9601 agttccatac cttgccggca gtggcaccga tctcttccgg tctccacgtc tccataacct
     9661 tcctcctggt cgctctctga gactgagatc catcatgaat ggatcatttt aatgtttcat
     9721 ttatcgaaag tttgtcaagt tcgtttggcc cccgtgaagc gccatttcgt ggggtggagg
     9781 gcagccattt tgttgacaca gttccgactt gacgccctcg aaacatccca tgtaatctgc
     9841 cggagagtga atggctgcgg agatttgtga gttgtcagtt aaggggcaaa aggaaatcat
     9901 tcatgagtaa cgacaaagta aagtcagtgc cggtctcgac cggggccgag gcgttcctgg
     9961 cgcaagtgcg cgcgctccgg gcggtccgct atctcttcgc caacaccgga actgatcacg
    10021 gtccgatcat agaagctttg gcaaaaaccg cgaccgaaga tcctaacgat cttcagccca
    10081 tcgtcgtccc gcacgaattg gcggcggtct ccatggccca tggttattac aacgtcacgc
    10141 agaagccgca aatggttttg gtgcatacgt tgcccgggac tgctaatgct ctgggtggaa
    10201 tcatcaatgc tcagtcggca aacgttccgg tgttgctggt ggcgggcaga acaccgatca
    10261 ccgaaggaga gctgcgcggt ggcaaaagcc agaatattca ttggcggcag gagtcgcgcg
    10321 atcaaggaaa catcgtgcgc gagttcgtca aatgggatta cgaggtgaga accaatcaga
    10381 acctcgccgc cgtggtatcg cgcgctttta agattgcgat gagcgagccg cgcggtccgg
    10441 tctatttaac cttgccccgg gagtggctgt gcgaagagtt ggagtcgacg gatttgtcat
    10501 cggattcttt gactgcgtcg acgaagatgc aagctgagta cgcggcattg gaacaggccg
    10561 cggagcttct cataagggcg gaacatccgc tgatcgtgac aaagtatctt gggcgcaatc
    10621 ccgaagcggt gccgctactc gttgagctgg ctgaactact cgccattccg gtcgtgcaac
    10681 aaatgagtta cctcaatttt cccaccgatc atccgctcta cctcggagtg caaacgattc
    10741 aacattccaa agaagccgat gtgctttttt tcatcgatac cgatgtgccg tgggaaccgc
    10801 caagccggcg ttttatccga tccgatgtga aaatcatcca tcttgagcgc gatccgctgt
    10861 tcaccggcat tcccggctgg ggcttccctg ccgactggcc gataacgggc tgctcggaag
    10921 tatcgttgcc gacactgacc tcgatcgtta gatcgaagct ccaaaagagc aagacgaagc
    10981 aaaatcgttt cgaagaacgc cgcaaaagga tagaagctga gcatcatgcg atatggcgtc
    11041 aagtcgaagc cgatatggat gcagtgaaaa atgaaaaacc catcagtccg ttgtgggtgt
    11101 caaagtgtat cggtgacgtg gttgacgaaa agacgatcat cgtcaacgaa accgtgacgt
    11161 cgaaactcgc cgatgtcatt catttgaacc gcccaggttc tcaattcggc acgccactgg
    11221 cgggccatct cggttggggt ctgggcgcgg cgatcggtat aaagttggga tctcctgaag
    11281 caaccgtgat cgcagcagaa ggagatggct cctatatgtt ctgcgcgcca actgcatgtc
    11341 atttcacggc acagaaatat cgcattccat ttctaacggt ggtctacaac aaccaagctt
    11401 ggaatgcgag cctcaacgcc gcgcggggac tctttcctga cggtttcgcc caaaaaagcg
    11461 gcaactttcc aggaaccgat ttgagtccct cgccaaagtt cgaattgacg gcgcaggctt
    11521 gcggcgcgta cgccgagcgt gtcgaagaac cggaagcgat cccggacgcg ctaaagcgag
    11581 cgttgaaagt cgttagggaa gaaaagcgac aggcgatgct caacataatt tgcaaagatc
    11641 ctttagcctg aactccttct ttgtctcagt tctaagacac ccttcggatg gacggccgct
    11701 tagaactcac gcctcattgt aaaaccccgg aaagtatcga attcgggtgt gccagtttta
    11761 gctcagccaa acttgacccc atgtttctaa accagcaatg tccatgaaag tggatttcca
    11821 ggcaggtggt tgcttgtagg aaaatcagta caagcgatct ccactggtga gcggccatcc
    11881 gcgataatct atttaaaatt caaggtgttg gaagccgttt tcagccaacc ggcacggctc
    11941 ttgccttagt cttgtgcatg gttagcggaa acgatcacga gacgaggtcg aatgtcacgc
    12001 aaccgaacgg aggagagatc ctcccggttg aagagatctt cgacgaaagt cctcgtagaa
    12061 acgagccttc attggggacg atccttttac tcatttcctc actcttgcag gcgggaagtg
    12121 ccctgccgga atctccgcta gatttgaagc cgcaaacggc ggaagcggca acggtgggtg
    12181 attcaaccgt tgccgcaaaa aataagcggc ctcaacaaaa aggtacacgt caaacaaaaa
    12241 gccaggaggc tttgaagaaa aaaatgctac gcggccaagc ctcttggtat gggcccggtt
    12301 ttcacggaaa gaaaaccgcc agtggtgaaa tctttgacca gaggcggtta actgcggcgc
    12361 ataaaacgct tccgctgggc acaatagcaa aggtcacaaa tttggagaat ggaaatactg
    12421 tagaagtcga gatcaatgat cggggaccct atgtcggcga acgggtcatc gatgtctcgc
    12481 gtgcggccgc taaggcactg ggcttcatcg actcgggaat aacattggta cggattgagc
    12541 ctttgtatca ggaatctggc acggaagaag cgggttgatg tagcaatgtc attggttcga
    12601 aagtgtcgta tgtgtttgag aggagaaacc taatagcaaa aggagaatat tatgcttagc
    12661 tggagcatca catttttagt cattggtctc atcgctggtc ttttaggact gtccgggatt
    12721 gccggcgcgg caactcagat agcctgggtt ctgttcgtcg tatttttggt tctgttcttg
    12781 gtgagcttag tgatggggag gcgttcgccg cctgtctgat gtcagcgata cgaatagcta
    12841 gttgtcgagg ccgggtcgac ggctaccgat ttagtcgtcg acccgcttaa ttctctgaag
    12901 tattgattcc cctataagcg ggaatctccg tgtgtgccgt cgttatcaac taacgacccc
    12961 g
//
LOCUS       DASUVS010000259         4306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1198848, whole genome shotgun sequence.
ACCESSION   DASUVS010000259 DASUVS010000000
VERSION     DASUVS010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198848"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VF182_14265"
     CDS             <1..282
                     /locus_tag="VF182_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438300.1"
                     /translation="DVPANTVPDMERIAKCESGGTHYRQDGRLVKTLNKNETMEFGNF
                     QINQLAKPKVASIVYNINLPEENDVFAYRLYDTSSTGDCHSSKHVGLYK"
     gene            436..1425
                     /locus_tag="VF182_14270"
     CDS             436..1425
                     /locus_tag="VF182_14270"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438301.1"
                     /translation="MPASLKIYLLVLSGIVAAVVATHAQAQSVAPAEKVVVSYPSKSI
                     TNFPILETARQQGFFQREGLQVSLVYIRGGLDIQTLLTNDADFAMGSTTAVTAFVAGA
                     PLRIVLSYNAHVDQGLYAQAKYRRIADLKGQPIGSLNPGGLVDTMVRRILIKNGVQPE
                     RDVNLLAMGGTPERFAALRAGAIAATMLSSPFNFLADKEGFSKLATTRDYVDVPGTAV
                     VVNAEKVKKQPNTVKRFIRASLRAMNYIRDNRADTTQLLAREFSMDQETAALAYKQLL
                     DLLSPDGRNRVSGYQLLVDFARAAQKMDRPMNAAQLIDETLLDEVMREGGISK"
     gene            1680..2720
                     /locus_tag="VF182_14275"
     CDS             1680..2720
                     /locus_tag="VF182_14275"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438302.1"
                     /translation="MTHPKVIDADGHIYENHEEIEEYFEGKYRGTRRARAYSLFPSLD
                     GWPRGLGAGRPDKVLETRPQDVVQFVENMGLESTVLYPTAALAIGLVQDPNWACSISR
                     AYNNWFHARYSKADSRLKGVAVLPVQDPHGAVQELRHAVEKLGMVGAFLPSATVMNKA
                     FGHPDFNPIFGAAEELGVPLGIHGAPSRGYGFDYFEDQGQVHALEHPFPLMIQFTSII
                     CEGVLEKFPRLKLAFLEAGCGWLPYMMDRLDYEYETRGGRSFPNMKKKPSDYVRDCAV
                     YVTCEPEETSLAYVSQVIGDDRLMFPSDYPHERVYGEFLKDIPELLARPELSDSTKQK
                     ILYDNAKEFYRI"
     gene            2731..3735
                     /locus_tag="VF182_14280"
     CDS             2731..3735
                     /locus_tag="VF182_14280"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6438303.1"
                     /translation="MSLLMQTIALLIFWSVATAAFAQDAKLIEAAKKEGRLVLYGTMQ
                     TDILELLQKGFQKKTGIAIDYWRSSATKVTERALSEARAGKALFDLVMSTEDTTRIML
                     KEGILAKYDSPLSKDFPSDAIDPELGPRARNHIIGIVYNKNIIKPGDAPKTVEDLVKP
                     QYRGKLVMADPTLHTTTAQWLANVHKLIGKEKSEKFIRELAAMKPTLVESFNPAADRI
                     TSGEFPIGITYVYYVYLNGIKGAPIDYARTGKFLGDASYLTLFRKAPHPNAAKAFIDF
                     FLDEESMSIMAKAGEFVNRKGIYPPVPDADKIQFVEMDDLGEKVYAEKRKEFHKLFLQ
                     "
     gene            3829..4299
                     /locus_tag="VF182_14285"
     CDS             3829..4299
                     /locus_tag="VF182_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012412603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4385 domain-containing protein"
                     /protein_id="HEX6438304.1"
                     /translation="MTFDYSLDFSTIDFRKQPELYRIGRGEQGVLLVEPYKSEILPHW
                     KFKTPQVARNSASKIYKMFLDYQAKDDFVGMDMARKFLQMGYTRSRRYANHKSGRKYA
                     AKTKELLPREEDHEKALSAQIFYKNWQKARADKKYLRSRNQHLKLYENTGKLRK"
BASE COUNT         1102 a         1150 c         1131 g          923 t
ORIGIN      
        1 gacgtccccg ccaacacagt ccccgacatg gagagaatcg ccaaatgcga atctggcgga
       61 actcactaca ggcaagacgg aaggctcgtt aagaccctca ataagaatga gaccatggag
      121 ttcgggaact tccagatcaa tcagctggcg aagccaaaag tcgcttccat tgtctacaac
      181 atcaatcttc cggaagaaaa cgatgtcttt gcgtaccggc tgtatgacac atctagtacg
      241 ggagattgtc acagctcgaa acatgttggt ctatataaat aatgcaatta caaccgagtc
      301 gcgcatcgac gccaggctgt ttgtcgttga cattttgaga gctcatcgac tattgaggat
      361 gtcacggagg agaaagcgtt cggatcgccc tttgtacgtt aagtgtcacg gcatcgactc
      421 ggcacgagag gaaccatgcc ggcctcatta aaaatctatt tgcttgtgtt aagcggtatt
      481 gtcgctgccg tcgttgcaac gcacgcccag gctcagagcg tcgcgcctgc cgagaaggtt
      541 gtcgtcagtt accccagcaa atccatcacc aacttcccta ttttggagac cgcgcggcag
      601 caaggtttct ttcaaagaga aggacttcaa gtaagcctgg tttatatccg cggcggtttg
      661 gatatccaga cgttgctcac caatgacgcc gattttgcga tgggcagcac caccgcggtg
      721 accgcgttcg tggcgggggc gccattgcgc atcgttttga gctacaacgc tcacgtcgat
      781 cagggattat acgctcaggc caaatatcgc cgcattgccg acctcaaagg ccagccgatc
      841 ggttcgctca atcccggcgg gctggtcgat accatggtgc ggcgcatcct tatcaaaaat
      901 ggcgtgcagc cggagcgcga tgtcaatctc cttgcgatgg gcggcacgcc ggaacggttt
      961 gcggcgctgc gagccggcgc catcgccgcg acgatgttga gctcgccgtt taatttcctt
     1021 gccgacaaag aaggcttttc gaagctcgct acgacccgag attatgtcga tgttccgggc
     1081 acggctgtgg tcgtgaatgc cgagaaggtc aagaagcagc ccaacacggt aaaacgattc
     1141 atccgcgctt cgctgcgcgc gatgaattat atccgggata accgcgccga taccacgcag
     1201 ctcctcgccc gggaattcag catggaccaa gagactgcgg ctttggctta caagcagctc
     1261 ctggatttgc tcagcccgga tgggagaaac cgggtgagcg gatatcaatt gctcgtcgac
     1321 tttgcccgtg ccgcgcaaaa aatggaccgg ccgatgaacg ccgcgcagct catcgacgaa
     1381 acactgctcg atgaagtcat gcgcgaaggc ggaatttcga agtgaagaca aacgtagtct
     1441 caatcgtttt ccaagagatt tcttgattgt ggccggacct tggatctacc atctgggacc
     1501 gtacttcttg aaatcgaaaa ttccaagaat tcctattcct tgaacggttg aaccttgaac
     1561 ttttgaacag ctgcggtctt caaaggcagg aaccgtgacc gggcttcgct gttccttgac
     1621 acccgaattg gcacagtcta cggtcagcca aatcatccag aacagattgg gaggactgca
     1681 tgacacatcc caaggtcatc gatgccgacg ggcacatcta tgagaatcat gaagagatcg
     1741 aggagtattt cgaaggcaaa tatcgcggca cgcgccgggc gcgcgcctat tcccttttcc
     1801 cgtctctcga cggttggccg cgcggtctgg gcgccggacg tcctgataaa gtgctcgaaa
     1861 cccggccgca agatgttgtc caatttgtcg agaacatggg gctcgaaagc accgtactct
     1921 atccaacggc ggcgctcgcc atcggtttgg tgcaagatcc gaattgggcg tgttcgatat
     1981 cgcgcgcgta taacaactgg tttcacgccc gctacagcaa ggcagactca cgattgaagg
     2041 gcgtcgctgt gctcccggtt caagatccgc acggtgcggt tcaagaactg cgccatgcgg
     2101 tggaaaagct cggtatggtg ggcgcattct taccgtcggc aacagtcatg aacaaagcct
     2161 tcggccaccc ggatttcaat ccgatctttg gcgccgccga agaactcggc gtgccgctgg
     2221 gaattcacgg cgcgccgagc cgcggttacg gcttcgatta tttcgaagac cagggtcaag
     2281 tccacgccct tgaacaccct ttcccattga tgattcagtt caccagtatt atctgtgagg
     2341 gcgtactgga aaaatttccg cgtctgaaac tcgcctttct cgaagcgggc tgcggctggc
     2401 taccctacat gatggatcgt ttggattacg aatatgaaac ccggggcggc cgctccttcc
     2461 cgaatatgaa gaaaaaaccc agcgactatg tgagggattg cgccgtgtat gtcacttgcg
     2521 aaccggaaga aacttctctg gcctacgtaa gtcaagtaat cggtgatgac cggctgatgt
     2581 ttccgtcgga ttatccacac gagcgcgtct atggagagtt cctcaaagac atccccgaat
     2641 tgctcgcccg ccccgagtta agcgattcga ccaagcagaa aatactctat gacaacgcca
     2701 aagaattcta ccgcatataa tggagagtca atgtcattat tgatgcaaac gatagcgctg
     2761 ctcatctttt ggagcgttgc gaccgccgcc ttcgctcagg atgccaagct gatcgaagcc
     2821 gccaagaagg aagggcggct ggtgctttac ggcaccatgc aaaccgatat tcttgagttg
     2881 ctgcagaaag gttttcaaaa gaaaaccggc attgccatcg attattggcg cagttcggcg
     2941 accaaagtca ccgagcgggc gcttagcgaa gcgcgcgccg gcaaagcgct ctttgacctc
     3001 gtcatgtcca ccgaggacac gacccgaatc atgctcaagg aaggtatcct ggcgaagtac
     3061 gactcgccgt tgagcaaaga cttccccagt gacgcgatag atcccgagtt aggcccgcgc
     3121 gcgcgcaacc atatcatcgg cattgtctac aacaagaaca tcatcaaacc cggcgatgcg
     3181 ccgaagacag tggaagatct cgtcaaacct caataccgcg gcaaactggt tatggccgac
     3241 ccgacactac acacgaccac ggcccaatgg ctcgccaacg tgcacaaact gatcggcaaa
     3301 gagaagtccg aaaagttcat tcgagagctg gccgcgatga agccaacctt ggtcgaatcc
     3361 ttcaatcccg ccgcagaccg gatcacctcg ggagagttcc ccatcggtat aacttacgtt
     3421 tattacgtat atcttaacgg catcaaagga gcgcccatcg attacgcacg gaccggtaag
     3481 tttcttggcg atgcgagcta cttgacgttg tttcgcaaag cgccgcaccc caacgccgcc
     3541 aaagctttca tcgatttctt cctcgacgaa gagagcatga gcatcatggc caaagcaggc
     3601 gaattcgtaa accgcaaggg tatttacccg ccggttccgg atgcagacaa gattcagttc
     3661 gtcgaaatgg atgacttagg tgaaaaggtg tacgctgaga agcgaaaaga atttcacaag
     3721 cttttccttc aataataacc aaattataat tcagcgattt gtttggacac gatgatacga
     3781 tcgttagaag cgatcgccga gggcgcaccg ggtatctcgc caagctgaat gaccttcgat
     3841 tactcactgg atttctcgac gattgatttc agaaagcaac ctgaactcta ccggatcggt
     3901 cggggcgagc agggagtact tctcgtcgaa ccctataaga gtgaaatcct gcctcattgg
     3961 aaattcaaaa caccgcaagt ggcgcgcaac tcagctagta agatctataa gatgtttctc
     4021 gactatcaag caaaagacga ctttgtcggg atggacatgg ctcgcaaatt tctgcagatg
     4081 ggttacacga gatcacgaag atacgccaat cataagtcgg gccgtaagta cgccgcgaaa
     4141 actaaagaac tactgcctcg agaagaagat cacgagaaag cattatcggc gcaaatcttt
     4201 tataagaact ggcagaaggc cagagcggac aaaaaatatc tacggtcgag gaatcagcac
     4261 ctgaagcttt atgaaaatac agggaagttg aggaagtgag agatca
//
LOCUS       DASUVS010000260         4958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1198913, whole genome shotgun sequence.
ACCESSION   DASUVS010000260 DASUVS010000000
VERSION     DASUVS010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198913"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1088)
                     /locus_tag="VF182_14290"
     CDS             complement(66..1088)
                     /locus_tag="VF182_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX6438305.1"
                     /translation="MEDLSPARIMEVGLGFWPSKVLLSAIELGVFTELGSNSMTGGEL
                     QVALQLHPRANPDFFDTLVALRFLERDGDGPGARYRNTEETAVFLDRRSPQFMGGFLE
                     MANARLYRFWGDLSEGLRTGKPQNEIKHTGAPMFVELYSKPERLEQFMDAMSGISAAN
                     FQAFADKFDFSGYHTLCDVGGATGQLSMLVANKHPHLHCISADLPAASRIADRKISAA
                     GLADRVSTTSLDFFAEPLPKADVITMGMILHDWNLEKKMQLIRAAYDALPAGGAFVVI
                     ENLIDDARRENAFGLLMSLNMVIEFGDAFDFTAADFFGWCHEAGFSRTEVIPLAGPAS
                     AGVAYK"
     gene            complement(1231..2253)
                     /locus_tag="VF182_14295"
     CDS             complement(1231..2253)
                     /locus_tag="VF182_14295"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX6438306.1"
                     /translation="MTRLNNRGSVLVFVTLTITLLLIMVGMGLDTGMLSYVRSQAQPA
                     VDAAALAAVSGLISGQAEVESRVAAYNATNDYTGQPASSLGSTNITPVFYDGSSIELA
                     ASYGTANGVRVGLEAAGSNPYAANTQSSIASPLFLTPLLNLMGYSAPTTANLNVTATA
                     VLQKRPELPFTIVGCTVGDFTASWSQTPSISDNSAFTSFTQGSANVNTMRDMVSASCG
                     TIPPVGIGSCININNGQDTPVLQEIVKAFGPAKAPFTDPPKDPEDCFLIPVIPDLPIG
                     GNLNKCRPMESLARLCITHVNAPGQPGFSPPMSISGRITDCNVPLYGVTQCSIPVLVR
                     DTKSGM"
     gene            complement(2280..2774)
                     /locus_tag="VF182_14300"
     CDS             complement(2280..2774)
                     /locus_tag="VF182_14300"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX6438307.1"
                     /translation="MDLPAEDACVPNNLARAGRRHSNGQSIIELTLIVPLVLATLYIP
                     ADFGVAFFTAQMVQNATREAARIGASMNPFVAGTVENEATKRLPGGKFVTASVSATLN
                     GSGTSTCMRRVVVTVSGSYSLFWYRLLNLMLPGAVTDTSLPITRSTAMRYDSQALTNT
                     GSCS"
     gene            complement(2758..3441)
                     /locus_tag="VF182_14305"
     CDS             complement(2758..3441)
                     /locus_tag="VF182_14305"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX6438308.1"
                     /translation="MAILLWDLIIAAAFVLVAVGALAYVLADLVVAAIHKAAGKKITG
                     LISAHIPKRPQRQPGHRLRARFSSIVFQKFICSLSAFKRAARKNLARKASGQALVEFT
                     LMFVLFLAIIWIPVDFGLAFYSGQLAQNASREGARLAAATNPFDAGNIQTQVMNRMDS
                     AVLKNVTADVVPPACDGALNMQVVTVTVAGEYNFYFYQLLRLLGLSAPDSVFISRTTT
                     MRYEYGFTC"
     gene            complement(3798..4124)
                     /locus_tag="VF182_14310"
     CDS             complement(3798..4124)
                     /locus_tag="VF182_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438309.1"
                     /translation="MAALAPDPSGSLAYPLSLFLAFALIVLSLWRVNTGLVEQQSARS
                     ASDITMTSLAPAHEDLLSDRHPASLEDLAFGSWGTIPMESENEAVRRKATVALMVYAT
                     GNGIKR"
BASE COUNT         1143 a         1312 c         1376 g         1127 t
ORIGIN      
        1 gtcgctcatc ggattctcga gattgagagg tgttcttaaa gctcccgatc ctactactgc
       61 tgcgattatt tgtatgcgac accggcgctt gcggggcctg ccaatggaat cacctccgtt
      121 ctgctgaagc ccgcttcgtg acaccagccg aagaagtcgg cggccgtgaa gtcgaaggca
      181 tcgccgaact cgatcaccat attgagcgac atcagcagcc cgaaggcgtt ttcgcgtcgc
      241 gcatcatcga tcaggttctc gatcaccacg aacgcgcccc ctgcgggcaa ggcatcgtaa
      301 gctgcgcgga taagctgcat tttcttttcc agattccagt cgtgcagaat catgcccatg
      361 gtaatcacat ccgcctttgg cagcggctcc gcaaaaaaat caagacttgt agtcgatact
      421 cgatccgcaa gtcccgctgc cgagatcttg cggtcggcga tccggctggc ggccggaaga
      481 tcggccgaaa tgcagtgaag gtgaggatgc ttgttggcca cgagcatgga taactgcccg
      541 gtcgcgccgc cgacgtcaca gagggtgtga tagccggaga aatcgaattt atcggcgaaa
      601 gcctgaaaat tggccgcgga aattccgctc atcgcatcca taaattgctc aagccgctcc
      661 ggcttgctgt agagctcgac gaacatgggc gcgccggtgt gcttgatctc gttttgtggt
      721 ttgccggttc gcagcccttc gctgagatca ccccagaatc gataaaggcg agcattggcc
      781 atttccagga agccgcccat gaactgcggg ctgcgccggt cgaggaaaac cgccgtttct
      841 tcggtattgc gatagcgcgc tcccggccca tcgccgtcgc gctcgagaaa acgcagcgcc
      901 accagagtgt cgaagaagtc cgggttggct ctcgggtgca actgaagggc aacctgtagc
      961 tcgccgccgg tcatcgaatt tgacccaagc tctgtaaaaa ctcccaactc gatggcggaa
     1021 agcaggacct tcgacggcca gaaacccaag ccgacctcca taattcgggc tggagagaga
     1081 tcttccataa cggatgctcc tttctgactg atcacggtca gcagcttcgc gccagcggtg
     1141 atgtggggtc gagattagac tgccgttggg acgagtgctc aagaaagatc ccgtaacttg
     1201 tctttcttgg cgatgcaagt ccctcggcac ttacatgccg gatttcgtat ctctgacgag
     1261 aacggggatg gagcactgtg ttacgccgta gagcggaaca ttacagtcgg tgatccttcc
     1321 agagatgctc atgggcggcg agaagccagg ctggcccggt gcgttcacgt gggtgatgca
     1381 caatcttgcc agcgattcca tggggcggca tttgtttaga ttgccgccaa tagggagatc
     1441 gggaatcacc ggtatcaaga aacagtcctc gggatctttt ggcgggtcag tgaatggagc
     1501 cttggcggga ccgaaggctt tgacgatttc ctgtagaaca ggggtgtcct gcccattatt
     1561 gatattgata cagctgccaa tgccaacggg agggatcgtg ccgcaactgg ctgataccat
     1621 atctctcatt gtgttcacgt tcgccgagcc ttgggtaaag ctcgtgaacg cggaattgtc
     1681 gctgatactc ggggtttggc tccatgaggc ggtaaagtca ccgaccgtac agccgacgat
     1741 ggtaaacggc agttcgggtc tcttctgaag gaccgcggtt gccgtcacat tcaggttggc
     1801 tgtggtagga gccgagtatc ccattaggtt caacagcggc gtcaaaaaga gcggtgaagc
     1861 gatcgagctt tgcgtattag ccgcataagg attgctgccg gccgcttcca atccgactcg
     1921 caccccgtta gctgtgccgt aggatgcggc gagttcgatc gaagatccgt cgtaaaaaac
     1981 aggagtgata ttggtggagc ccagactact ggctggttgc ccggtataat cattggtcgc
     2041 attgtaggcg gcgactcgcg actctacttc tgcttggccc gatatcaagc ccgacacggc
     2101 ggctagggca gccgcatcca ctgcgggttg tgcctgactt ctaacatagc tcagcatccc
     2161 tgtatccaat cccatgccga ccatgatgag cagcaaggtt atcgtcagcg tgacaaaaac
     2221 caaaaccgag ccccggttgt tcaagcgagt catcgtagct ccttcgtaaa gtgtcgccat
     2281 caactgcagg aaccagtatt cgtaagcgcc tgggagtcgt agcgcatcgc ggtgcttcgg
     2341 gttatcggca gcgaagtgtc tgtgacggca ccggggagca tcagattcag caggcgatac
     2401 caaaacagac tgtaactgcc gctaaccgtg acgactactc ttcgcatgca agttgaagtg
     2461 ccgctgccat ttaaagttgc cgacacactg gcggtcacga acttcccgcc cggaagacgc
     2521 tttgtggctt cattctcgac cgttccggca acgaatgggt tcatcgaggc gccgatccgc
     2581 gcggcttcgc gggtagcatt ctgaaccatc tgggcagtga agaacgcaac gccaaagtcc
     2641 gccggaatat ataaggtcgc aagcaccaga ggcacgatca aggtcagttc gatgatactt
     2701 tgaccgttgg agtggcgcct gcctgctctt gcaagattgt tcgggacaca agcatcttca
     2761 gcaggtaaat ccatattcat acctcatcgt agttgtccgg ctgatgaaaa cgctgtcggg
     2821 agcagaaaga ccgaggagcc gtaataactg atagaaataa aagttgtact cgccggctac
     2881 cgtaaccgtg accacctgca tattcagcgc gccgtcgcat gccggcggaa ccacgtcggc
     2941 agtgacattc ttcaataccg cgctatccat tctattcatg acctgggttt gtatgttgcc
     3001 ggcgtcgaag gggttcgtgg cggccgccag acgggctcct tcgcgggagg cattctgcgc
     3061 caattggcca ctataaaaag ccaggccgaa atccacaggt atccaaatga tcgcaagaaa
     3121 cagaacgaac atgagggtaa actccaccag agcttgcccg cttgccttac gagctagatt
     3181 ctttcgcgct gcgcgcttaa aagcggagag tgaacagatg aacttctgga agacgatgct
     3241 actaaaacgc gcccttagtc tatggcccgg ctgccgctgc ggtcgtttcg ggatatgagc
     3301 agagatcaga ccggtgattt tcttcccggc ggctttatga atcgcagcca cgaccaaatc
     3361 cgctagtaca tacgcaagag caccgacagc cacaaggacg aacgctgctg caatgatcaa
     3421 atcccatagc aaaattgcca tgtgagtatc tcccgcgtct gaatcaacaa ccgttcagaa
     3481 gcgccggtta gatatcacaa cgactttgct tttcaagggt tgagttttgc gccgtaagcc
     3541 gcagagccgc cggcgtatga gtaagcggtg gggcttgttg aatagggtcg attcatctct
     3601 ggacatgcag cgatcttcgg cgaggaagat ctcgcaagtt ggcgggcaaa gaggctattc
     3661 tttatgacga tcgaaactgg cgagcgtctt gttcgtgttc atctctcccg cgtcgcatct
     3721 tatacttcct gtaagagggg atgcagtcag gaggcaggac gaggaaacgc tgtcgggaat
     3781 aaaagaaaag ctcccggcta tctttttatc ccgtttccgg tggcgtaaac catcaacgca
     3841 acggtcgctt tgcggcggac cgcttcattc tctgattcca ttggaatcgt gccccatgaa
     3901 ccgaaagcaa ggtcttcaag agaagcgggg tggcgatcag agagtaaatc ctcgtgggcg
     3961 ggtgctaagc ttgtcatcgt gatgtcgctg gccgaccgag ccgactgttg ttccactaag
     4021 ccggtattca ccctccagag tgaaagcacg attagcgcaa aggccagaaa aagagataaa
     4081 ggataagcca aactgccgga cggatcgggg gcgagagctg ccataaatat ctccttgtca
     4141 cttatgggag gagttgtggg ctctaatctc ttgctatcac caaaccatgg aaacacaacc
     4201 cgtttagacg gggtttgtaa aataaagttg acatagttcc tggtctttta gagtcttcgg
     4261 tataagccgt tatgccgtcc tcggttgttc cgcgagaagt tatcctcggg cctcgtaaaa
     4321 ctgcgccgaa tcgctttcgg tttgatgttt acacatgcag tgcgccggcg gaagcttcgg
     4381 tcacattgtc aatttggtta ctgtcggttt gttcgtataa acttgacgca aacggcaacg
     4441 tgtgtctgtc caatattaga cgagtataaa ggatcgtggt gaggccgggt taccccgtat
     4501 gttatcgttg tacctttttt cttagcatac ccgaaatctt tgatatgatc ccgcgctgtg
     4561 ggctgggccg gctgtgtcat ttctatctcc gagcattgtc cgccaagagt cgtcgagtcc
     4621 gctaagatct aatttactgc aaacctatag tagccgcggg ttcggcgcct gtttgggata
     4681 ctcaaataag accatctgca ccgacaacgt attctgacac tgatgccgcg caacgatcaa
     4741 acactcgtct gtgcgataaa cgcatccttc atgtggtgcg tgaatcgata tgagatgccg
     4801 aaaggcgttg agcgcggcaa aaattcaatc ctctacccat gtttgcctct cagaggcaat
     4861 cgacctcgtg gctctacggc cgatctgtga taacaataat tcgatgcggg atcgcgtcgc
     4921 accgaatgtc tataccgaag ggctcaccca cacgtgag
//
LOCUS       DASUVS010000261        11917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1417471, whole genome shotgun sequence.
ACCESSION   DASUVS010000261 DASUVS010000000
VERSION     DASUVS010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11917
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417471"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..744)
                     /locus_tag="VF182_14315"
     CDS             complement(22..744)
                     /locus_tag="VF182_14315"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX6438310.1"
                     /translation="MINTERVTTGIPGLDQLLEGGFPKGRSVLVTGEPGTGKTIFALQ
                     FLVEGLRRGEKGIYVAADEGPTDIIEQAASLGWDLEKYIEAKDLAILNAGTYLSSSPG
                     TGKERQIDIHKAIGDLAGFVNRLGAKRLVLDPAGPFVLLRDTAARIQDQTRLLIKLLR
                     TSMQTTNILTSYGVPRTGERTLHGIEEYLVAGAIILEIVWSDGKLGRTMVIEKMRCTD
                     VKPGQHAFDINKVHGLVLEPLP"
     gene            complement(797..2029)
                     /locus_tag="VF182_14320"
     CDS             complement(797..2029)
                     /locus_tag="VF182_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438311.1"
                     /translation="MGKTFEVKIRPPNRWFDPAMTRRMELAALEKTMHNRDDCEIDEV
                     AALQQAIGAKELLLRERQAAFEALEERLNSTIEQLSADLNEKQTLIGQNEIKSQQAKA
                     EINEIVEQKTRLEMLQKQTERLLSAQAEQIRAGVRAEIQSLERQLVDKQLELHNCQAC
                     AADLQHRLAEMQLLSETRAVQIEDLKAENVRLSQEINHRQPVKLATGFCAGHEPSHGR
                     EAGLRANSHHSETAETSAGREIGTLRQDAEEKHLLLASRNEELMRVKSEMELLQGEVS
                     ELKSSSKRIQDSAETESAKMRTEFQAQLAFLQAELSQRDWALQEGQAAQKVLEHGLRA
                     KIDELEVQLAQLKSSSEASPQEFVLGDKWESEARLDHTWKMQERLDGNSAEGAQTAAN
                     DSGRWRSRGQWKRRWRSR"
     gene            2395..3489
                     /gene="hisC"
                     /locus_tag="VF182_14325"
     CDS             2395..3489
                     /gene="hisC"
                     /locus_tag="VF182_14325"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX6438312.1"
                     /translation="MSRYFRANIESMEGYVPGEQPGDEKLVKLNTNENPYPPSPQVLA
                     ALSEATNQSLRLYPEPLSDRLRNIAAHVYGTGLENILAGNGSDEILSIVLRCFVGRGD
                     RVAFPVPTYSLYETLVKIQDGEPVCLDYPADFSIPPELHSANAAVTFLCNPNSPSGTL
                     VRVTEIEKLARAVAGVLVVDEAYIDFADDPDSSAIPLVKSIPNLIVLRTFSKSFSLAG
                     LRIGLAFAPAELIAGMMKVKDSYNVNRLSLVAATAALQDLPWMQRNVGRIQRTRRELV
                     ERLGKLGFYVYPSHANFVMAQKKAQEQKALYEALRKRKIYVRYFDTPELRDSLRITVG
                     TPEDVDRLLQEIASIEKASLFPSQTAGGKS"
     gene            3486..3902
                     /locus_tag="VF182_14330"
     CDS             3486..3902
                     /locus_tag="VF182_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014029113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6438313.1"
                     /translation="MSGFKISELDHIVLNVGDIERSLQFYTEVMGLKVERLDEFRTGK
                     AGFPSVRINADTIIDLFPVQKTRTKESDGEKKAGNLNHFCMVTGKEDFAGIVDYLKEN
                     RIIIREGPISRWGARGRATSVYFLDPDGNEIEIRCY"
     gene            complement(3909..5234)
                     /locus_tag="VF182_14335"
     CDS             complement(3909..5234)
                     /locus_tag="VF182_14335"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX6438314.1"
                     /translation="MIVENFFFEAAVIFLLILANGFFAASEIAMIAIRKSRVDTMIEK
                     GVKRALIIGRLKKDPDRFLATVQIGVTIVSSLASALGGAAAISFVKPAIEASAIPALA
                     PWADVIAIAVVVIPISYLSLVFGELVPKSLALRFSEQIACMAARPIDLLSRVSSLLVR
                     ILTGSSNAVLRLFGGSDVEGASFISVEEVKSLIREGAAKGIFNETEKELIHGVFELAD
                     TPVKAVMVPRTEIHALEIHSTPSEAAASFIESGFSRIPIYDGELDHVIGVLYNKDFFK
                     TLQQKTEFRIKDHLHPAFFVPSTLPISELLQQLQRRRLAIAMVVNEFGEVEGLATLED
                     LLEEIVGEIRDEYDREERGPVDRLPDGSMVIQGSALLKDLKADFDLPFEESPDYHTLA
                     GFLLAKLKRIPRGGEWVEENGYKLTIVDMEGRRIVKIKLEQTNPHNRQL"
     gene            5530..7746
                     /locus_tag="VF182_14340"
     CDS             5530..7746
                     /locus_tag="VF182_14340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEX6438315.1"
                     /translation="MVKQPEKIAVQGTVHKITYHHPDTHYTVLRLDVENAPGVTVVGG
                     VYPVSEGEEIKVFGFWKTHSRYGQQFQADHWEKVDPATLEGIEKYLGSGMIKGIGPTY
                     ARRLVQAFGLETLKILSQQPHRVLEVEGIGASRARGIMQAWEAQRGMQDIMVFLQGHG
                     IGAALALKIYRFYGLDTIRRVKENPYALARDIYGVGFVLADRIAARLGISGDFPLRVQ
                     AGVLHVLKEFVDQGHCFVLFSALTRNAAALLRVGEGVIENAAEQLAATGDVILEQTTS
                     EERAHVYLSELYRAEQRVAQALHRLLSSPSVTLGGGKSGGSTAQPRELIGLADLQLID
                     QSFAVLEEEQQEAVRQALRQKVLVITGGPGTGKTTLLNSLLALLRRAKFSFVLAAPTG
                     RAAKRMAESAGEEALTIHRLLEYNPHEGGFQRSEDRPLDADVVIIDEASMVDLALMDN
                     LLSAIDRHSHLVLIGDVDQLPSVGPGSVLRDLIDSRMIPVVMLRRIFRQDRHSLIVAN
                     AHRILQGQSLVTPSDSSERDFILLARESEEEILQSVKDLVKEELPRTLNLAADEVAHA
                     IQLLTPMHRGMLGTIQLNREMQSLLNPVGDSLQRGESLLRANDKVMQLRNNYDKGIYN
                     GDLGRITGIDRQEGKVQVDFYDKVVEYNDDELDEISLAYATSIHKSQGSEYPVVVIPL
                     HMSHYMMLHRSILYTAVTRGKKRVVLVGSRRALAMAIRNVRLERRYTGLKEKLAKL"
     gene            complement(7817..8518)
                     /locus_tag="VF182_14345"
     CDS             complement(7817..8518)
                     /locus_tag="VF182_14345"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438316.1"
                     /translation="MKAQARIGLIIPSSNRLTEPQFNRYAPPGVDIHVTRLRMTGKYR
                     KPLDELRRSLIEASEALSDINPGMIVFHCTANSMENGLAHEAAIVEIIEQSSGCPTLT
                     TAQAITRAFNHFAIKTLVLISPYVQATNEHEVHYLKEAGYTVVHELGLGLESHGYSAV
                     TPEEWKQIVMENTRADADGYFLSCTNTRMIEAVAEIEREIDKPVISSNQATLWACLKR
                     LGISHSDNRLGRLFN"
     gene            complement(8515..9609)
                     /locus_tag="VF182_14350"
     CDS             complement(8515..9609)
                     /locus_tag="VF182_14350"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438317.1"
                     /translation="MGFKGYTIIDGDGHVIEDTKSMVGFMPQPYRDKFDTHPFFNPFP
                     PLDHLHSANMHDFQPGAFNKVGPEGWLDFLEDVGIETTVLYTTLGLAFGKIVSRDWAI
                     DVARGYNDWLYHEYLKKSPRFKGIALIPLQETQAAVDELRRAVKELGMCGAMLPSTGI
                     QSNLGDQRYWPIYEEADRLGCALGVHGGAHENMGLDDLSPYAPVHALGHPFGQMISFA
                     GMVFNGIFDKFPNVKFGFMEGGVAWLLMCLERFDRSWETHIQHDPRKRFLNLRPKEKV
                     REYISRHIDEGRIFIGCEGEEPDIAYAIERVGNKPFVFSSDYPHEVNNEFCKHEITEM
                     LENKEMTEADKKAVLHENARRFYSLGTPAL"
     gene            complement(9802..10197)
                     /locus_tag="VF182_14355"
     CDS             complement(9802..10197)
                     /locus_tag="VF182_14355"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438318.1"
                     /translation="MQDGGESILTVATSVPQNQVSFYRDYAGRIGTIDRRITFTAVGT
                     SLRVAANALADKQIRVRLTPRISYFSAEHSGAIDFTEAMTEMIVPNGQPVSLGGSTSR
                     IHELTRQILGYNERASSSESELIITATLQ"
     gene            complement(10222..10458)
                     /locus_tag="VF182_14360"
     CDS             complement(10222..10458)
                     /locus_tag="VF182_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438319.1"
                     /translation="MLRKKSTLLNIVLYFTFALLVLAPAGAQTSRHIKVLVDSQQRVT
                     GNQEEVQGSGSIIIRQGGAQPSARVTAGNRQTTV"
     gene            complement(10620..11588)
                     /locus_tag="VF182_14365"
     CDS             complement(10620..11588)
                     /locus_tag="VF182_14365"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438320.1"
                     /translation="MNRKSPPKASNPLVFVFLAFVLFLSSSVRAAEGLQKIRVGFPSL
                     AFSYMPYYVAQEKGVLKKYGLEAEYIQMRTGIQPQAVINGNINFFPSVSTGISAAVSG
                     LPLMVVLNFCDGAPWILVTQKDINKPQDLIGKQVAVSGLRTSPHYFLQAALKKWEIAE
                     KDIGIISTGGTADSFMALASNRVAGTVLTPPFDDKAVSLGYKKFMFLGDLADIPYVGV
                     VTSQAQVKNNRETVRRTLAALSDSVGWIRANRTESSKMIADKFKVTAMEAEQTYNTLL
                     KILNKDGRLDPKVARGYLELLRQERPIAADFDPQKVLDFSLLPGNR"
     gene            complement(11605..>11917)
                     /locus_tag="VF182_14370"
     CDS             complement(11605..>11917)
                     /locus_tag="VF182_14370"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438321.1"
                     /translation="EIIRGEQVVLTCESEETGLDRVFAANGENTVLYASDYCHWDCHF
                     PYSVKDVVDGEDLTFPEKERLLNRNAIEFFKLKNLPQPAALKKARESWASIGQQKIAT
                     A"
BASE COUNT         2710 a         3214 c         3203 g         2790 t
ORIGIN      
        1 gtgattgtta cctggagagg tttagggcag aggctcgaga acgagtccgt ggactttgtt
       61 tatgtcaaac gcgtgctgcc ccggtttgac atcggtgcag cgcatttttt cgatcaccat
      121 ggtgcggccc aacttaccat cactccaaac gatctccagg ataatagcgc cggcaacgag
      181 gtattcctct attccatgca gagtccgttc gccagttcga ggcacgccgt aggaggtgag
      241 aatgttggtt gtttgcatcg atgttcgcaa taacttgatc agcaagcggg tctgatcctg
      301 gatgcgggcc gcggtgtcac gcagcaggac aaagggccca gccggatcca gcaccagccg
      361 ttttgctccc aagcggttga cgaagccggc aagatcgccg atcgctttat gaatatcgat
      421 ctgccgctct tttcccgtgc cgggcgagga gctcagataa gttccggcgt tgagtatcgc
      481 cagatccttc gcttcgatgt atttctccaa atcccatccc agtgaggcgg cctgttctat
      541 gatatcggtt ggaccttcat cagccgcgac gtagatgccc ttttctccac gcctcaagcc
      601 ttccaccagg aactgcagcg caaaaatcgt tttcccggtt ccaggctcgc cggtaacgag
      661 aacagaacgg cccttgggaa aaccaccttc aagaagttga tccaatcctg ggatgccggt
      721 tgtcactcgt tcggtgttta tcatgttgtc cttctagtga ataaatctat cgggtagagc
      781 gagttttgcg cgccgcttat cgcgatctcc agcgtctctt ccactggccg cggctgcgcc
      841 acctgccaga gtcattggcg gccgtctgcg cgccttcggc gctgttgccg tcaagccgtt
      901 cctgcatctt ccacgtgtga tccaaccgcg cttctgactc ccacttatcg ccaagaacga
      961 actcctgagg cgaagcctca ctggaacttt ttagctgcgc cagctgaact tccaattcgt
     1021 caattttcgc gcgaagtccg tgctcgagaa ctttctgcgc tgcttgacct tcttgcaacg
     1081 cccaatctct ctggcttagc tccgcctgta aaaaggccaa ttgagcttga aactcggtgc
     1141 gcatctttgc gctttcagtt tcggccgagt cttggatacg cttactgctc gatttgagtt
     1201 cgctaacttc gccttgaaga agctccatct ccgacttcac gcgcatcagt tcttcattgc
     1261 ggctggcaag gagaagatgc ttttcttccg catcctggcg cagggtcccg atctcgcgcc
     1321 ctgccgatgt ttctgctgtc tctgaatggt ggctgtttgc gcgcaacccg gcctcccggc
     1381 catggctggg ctcatgaccg gcgcaaaatc cggttgcgag ctttactggc tggcgatgat
     1441 taatctcttg tgaaaggcga acattttctg ctttgagatc ttcaatctgg acggctcggg
     1501 tttcactgag caactgcatc tccgccaagc gatgttgcag gtccgccgcg cacgcctggc
     1561 aattgtgaag ttccaactgc ttgtcgacga gctgcctctc caaagactga atctcggcgc
     1621 gaacaccggc gcggatttgc tccgcctgag cagataacaa acgttccgtt tgcttctgca
     1681 gcatctcgag ccgagtcttc tgttccacaa tctcattgat ctccgctttg gcttgctgag
     1741 atttgatttc gttttgacca atcaaggttt gtttttcatt caaatcggcg ctcagttgtt
     1801 cgatggtaga gttcaaccgt tcttccaagg cttcgaacgc agcctggcgt tcgcgcaaga
     1861 gcaattcctt agctccgatt gcttgttgca gggcggccac ttcatcgatc tcgcaatcat
     1921 cgcggttatg catagttttc tccaaagctg ccaattccat tcgccgtgtc attgccggat
     1981 cgaaccagcg attaggcgga cgaattttga cttcaaatgt tttgcccaag agggctgcgg
     2041 atcctcaggt aatcgaggac gtgatgcgtt gctcaaagaa aaatccccgg gggagcaatc
     2101 tccggtaatc tctttgaagt gcagcagctc ttcgtttcga gcgggcgaac acgcgcgcat
     2161 gtgccaaaaa catgccagga tcaaaaacaa aaacgattaa tattcagtag gttacaaaca
     2221 ggtgtggggc cggatcgtgt gaaatcgtgc acaaaggagc tattcctgga caaaatcgat
     2281 taattttgga caaaaacctt tgacgatcta aagggaagct tcgatgacgc cgagggcgcg
     2341 attttcctaa tcagcggttt gaaatgggtt tttgcggcag ctagaatccg cagcatgtca
     2401 cgttactttc gcgccaacat tgaatcgatg gagggctatg ttcctgggga gcaacccggt
     2461 gatgaaaaac ttgtgaagct caacacgaac gagaatccgt acccgccgtc gccgcaggtg
     2521 ctcgccgcgt taagtgaagc gacgaaccaa tcgctgaggc tttatccgga accgctcagc
     2581 gaccggttac gcaacattgc cgcgcatgtg tacggtaccg gcctggagaa cattctagcg
     2641 gggaatggct ccgatgagat cctgtcaata gtgttgcgct gtttcgtcgg tcgtggtgat
     2701 cgggtcgcat tcccggtgcc gacctattcg ctttatgaga cgctggtgaa gattcaagac
     2761 ggcgagccgg tgtgccttga ttatcccgcc gatttttcaa tcccgcctga gttacactcg
     2821 gcaaacgccg ccgtcacttt tttatgcaat cccaactccc catccggaac acttgtacgt
     2881 gtgacggaaa tcgagaagct cgctcgggct gtcgccggag ttctcgtggt agacgaggct
     2941 tacatcgatt ttgctgacga ccccgattca tcggctatcc ctttggttaa aagtattccc
     3001 aatctgatcg tgctgcgcac tttttcaaaa tccttttctc tcgctggtct gcgtatcggc
     3061 ctcgcttttg cccccgccga attgatcgcc ggtatgatga aggtaaaaga cagttacaac
     3121 gtaaaccggt tgagcttggt tgccgccacc gctgctttac aggatctccc gtggatgcag
     3181 cgtaacgttg gccgcatcca gcgcacccgc cgggaattgg tcgagagact gggcaaattg
     3241 gggttttacg tctatccgtc gcatgccaac tttgtaatgg cgcaaaaaaa agcgcaggag
     3301 caaaaagcgc tttatgaggc gctacgaaaa aggaagattt atgtgcgcta ctttgataca
     3361 cccgaactgc gagacagctt gcgcatcacg gtcggcacgc ccgaagatgt cgacagattg
     3421 ttgcaggaga tcgcatcaat tgagaaggcg tcgcttttcc cgtcccaaac cgcaggaggc
     3481 aaatcatgag cggatttaaa attagcgagc tcgaccacat cgtgctgaat gtcggcgata
     3541 tcgagcgctc attgcagttc tataccgagg tgatgggctt gaaagtggaa cggctcgacg
     3601 aattccgaac cggcaaggcc ggctttccgt cggtgcggat caatgccgat accatcatcg
     3661 atttgtttcc agtccaaaaa acccggacaa aggaaagtga cggggaaaaa aaagcgggca
     3721 atttaaatca cttctgcatg gtcaccggca aggaggattt cgccggaatc gtcgactact
     3781 tgaaagaaaa tagaatcatc atcagagaag gaccgatctc gcgctggggc gcccggggca
     3841 gagcgacatc cgtctacttc ctcgatccgg acggcaatga aatagaaatt cgttgttatt
     3901 gataaggatc aaagttgtct gttgtgcggg ttggtttgct ccagctttat tttgacgata
     3961 cgccttccct ccatatcgac gatggtgagc ttgtagccgt tttcctcgac ccattcaccg
     4021 ccgcgcggaa tgcgctttag cttagcaagc aggaaacccg caagcgtatg gtaatcgggt
     4081 gactcctcga acggcaaatc gaagtcggct tttaaatctt tcaacagcgc cgagccctgg
     4141 atcaccatcg aaccgtcagg caatcggtcc accgggccgc gttcttcgcg atcatattcg
     4201 tcgcggattt cgccgacgat ctcctccagc aagtcttcga gcgtcgccaa tccctcgacc
     4261 tcgccgaatt cgttgaccac catcgcaatc gcaagtcgcc gtcgttgcag ttgctggagc
     4321 agctcactaa tcggcagagt gctggggacg aaaaatgccg ggtgcagatg atcttttatc
     4381 cgaaactcgg ttttttgttg cagagtctta aagaaatctt tgttataaag aaccccgatc
     4441 acatgatcca gctcgccgtc gtagatgggg atgcgcgaga agccgctttc gatgaaactc
     4501 gccgcggctt cgctgggagt ggagtgaatc tcgagagcat gaatttcggt gcgcggcacc
     4561 atgacggctt tcaccggggt atcggccagt tcaaagacgc cgtggatcag ctctttttcg
     4621 gtttcattga atataccttt agccgctcct tcgcgaatca gggatttgac ctcctcaaca
     4681 ctgatgaaac tcgcgccttc gacgtcggag cccccgaaca accgaagcac cgcgttgctg
     4741 gagccggtca ggattcgcac aagcaatgag ctaacgcgcg acaaaaggtc gatcggccgg
     4801 gcagccatgc atgcgatctg ctcagaaaag cgaagcgcga gcgatttggg caccaactcg
     4861 ccgaatacca atgaaagata ggagattgga ataaccacca cggcaatcgc gatcacgtcg
     4921 gcccacggcg ccagggcggg aatcgccgat gcttcgatag ccggtttaac aaaggaaatt
     4981 gcggcagccc cgcccagggc ggacgcaagc gagctgacaa tcgtaacccc gatttgtacc
     5041 gtagccaaaa atcggtccgg atcttttttg aggcggccga ttattaaagc gcgtttgaca
     5101 cccttttcga tcatggtgtc aacccggctt ttacgaatcg caatcatggc gatttccgaa
     5161 gcagcgaaga aaccgttggc cagaattaaa agaaaaatga cggctgcttc aaaaaaaaag
     5221 ttttcgacga tcatcaatat ctctcctccg tgtcttcagc tcggagacaa cggaaaacac
     5281 ggttttgccg tgagtatcac caggaggcaa cgttctggaa aggataggtc aggtgcggag
     5341 ggcgcatatc tgctagattc gaatgtaagc atccgctctc gaaaacgcaa gcacttgtta
     5401 tatatagcca aagtcgagca aaagactcga cacgggtcaa aacagacggc ggttggacgc
     5461 aagcacacaa gcccccgcag aggctcaggg gatgatcgtt caatccaaga aggctaggaa
     5521 caagatcctg tggtaaaaca gcctgaaaaa attgcggtgc aaggcaccgt gcacaaaatc
     5581 acctaccatc atcctgacac tcactatacg gttttgcggc tggatgttga aaatgcgccc
     5641 ggcgtcacgg tggttggcgg tgtctatccc gtttcggaag gcgaggagat caaggtcttc
     5701 ggtttctgga agacgcactc acggtatggc cagcaatttc aagccgatca ttgggaaaag
     5761 gttgatcccg caactttgga aggcatcgaa aaataccttg gctcgggcat gatcaaaggc
     5821 atcgggccga cctacgcgcg ccgtttggtg caggctttcg gattggaaac tctgaaaatc
     5881 ctctctcagc agccgcatcg tgtcctcgaa gtcgaaggga tcggcgcgtc gcgtgcccgc
     5941 gggatcatgc aggcctggga agcgcagcgc ggaatgcaag atatcatggt cttcttgcaa
     6001 ggacatggca tcggcgcggc cttggcgctc aaaatctacc ggttctacgg gctggacaca
     6061 attcgacggg tcaaggagaa cccctatgcg ctggcgcgcg atatctatgg cgtcggtttt
     6121 gtcctggccg accgcatcgc cgcgagactt ggcatcagcg gtgatttccc ccttcgtgtg
     6181 caggcgggcg tgctacatgt cctcaaagag tttgtcgatc aagggcattg cttcgtgctt
     6241 ttctcggctc ttaccaggaa tgccgcggcg ctgctgcggg tcggcgaagg tgtcatcgag
     6301 aatgcagcgg aacagctggc tgcgactggc gacgtgatcc ttgaacagac aaccagcgag
     6361 gagcgggcgc atgtctatct ctccgagctt tatcgtgccg agcagcgagt cgctcaagcg
     6421 ctgcaccgtc tattgtcgag cccttctgtt accctgggcg gtggcaaatc cggcggatca
     6481 acagcccaac cacgcgagtt gatcggattg gccgatctgc agctgataga tcaatccttt
     6541 gctgtcctcg aggaggaaca acaggaggcg gtgcgccagg cgttgcggca aaaggttctg
     6601 gtcatcaccg gaggaccggg aaccggcaaa accactttgc tcaactcatt attggcgctc
     6661 ttgcgtcgcg cgaaattttc attcgttctt gccgcgccaa ccggacgtgc ggccaaacgc
     6721 atggctgaaa gcgccggcga agaagccctg acgattcacc ggttgctcga atacaatcca
     6781 catgaaggcg gctttcaaag gagcgaagat cgaccgctgg acgctgacgt ggtgatcatc
     6841 gacgaagcct cgatggtcga tctggcgctg atggataatc tgctcagcgc catcgatcgc
     6901 catagtcatc tggttctcat cggcgatgtc gatcagcttc catcggtggg accgggcagc
     6961 gtgctgcgcg atttgatcga ttccagaatg atacccgttg tcatgttgcg gcgcatcttc
     7021 cggcaagatc gccatagcct gatcgttgcc aacgctcatc gtattctcca gggacagtcg
     7081 ttagtaacgc cgagcgatag ctcggagcgc gacttcattc ttctagcgcg ggaatccgaa
     7141 gaagaaattc tccaatcagt caaggatctc gtcaaagagg agttgccgcg aactctgaac
     7201 ctcgcagctg acgaggtcgc gcatgccatc caactcttga cgccgatgca taggggcatg
     7261 ctcgggacga tccaactcaa ccgagagatg cagagcttac tcaacccagt cggcgattcg
     7321 ctgcagcgcg gcgagagctt actccgagcc aacgacaagg tcatgcagtt gcgcaacaac
     7381 tatgacaaag gtatttacaa cggcgacctg ggccggatca cgggcatcga tcgccaggag
     7441 ggaaaggttc aggtcgattt ctacgacaag gtagtcgagt ataacgatga tgagctggac
     7501 gaaataagcc tggcttatgc cacatcgatt cataagagcc agggcagtga ataccccgtg
     7561 gtggtcatcc cgctgcacat gtcccactat atgatgctgc atcgaagtat tctttacact
     7621 gcggtcacgc gaggcaagaa aagagtggta ttagtcggca gccggcgcgc tctggccatg
     7681 gcgattcgaa atgttcggtt ggaacggcgc tatacggggt taaaagaaaa gctggcgaag
     7741 ctataacgca caggtggccg ggaaggccgt cgctaagtac cggcgcgact tgaatccgag
     7801 tggaaactgc aacgcgctaa ttaaaaagac gccccagcct gttgtccgaa tgcgatatgc
     7861 ccaatcgttt caggcacgcc cacaaggtcg cttggttgct gctgatgacc ggtttgtcga
     7921 tttcccgctc aatctcggcg accgcttcga tcatccgcgt gtttgtacaa cttaggaagt
     7981 aaccgtcggc atccgcccgg gtattttcca ttacgatctg cttccattcc tccggagtca
     8041 ccgccgagta gccgtgactt tcaagtccga gacctagctc gtggacgact gtgtagccgg
     8101 cttccttgag atagtggact tcgtgttcgt tggtcgcttg cacgtaggga ctgatgagca
     8161 ccagtgtttt aatggcaaag tggttgaagg cccgggtgat tgcttgcgct gtagtgaggg
     8221 tgggacagcc gctggattgt tcgatgattt caacgatcgc ggcttcgtgc gctaatccat
     8281 tctccatcga gtttgcggtg cagtgaaaaa caatcatccc cggattgata tcggaaagag
     8341 cttcggaggc ttcgatcagg gaacgccgca actcatccaa cggcttgcga tatttaccgg
     8401 tcatgcgcag ccgggtgaca tgaatgtcga cgcccggcgg cgcataacgg ttaaactgcg
     8461 gctcggttaa tcgattgctc gaaggaatga tcaaaccaat gcgcgcctga gccttcatag
     8521 cgccggtgtg cccaggctgt agaaccggcg ggcgttttca tgcaggaccg ccttcttgtc
     8581 cgcctctgtc atctccttat tttccagcat ttcggtaatc tcatgtttgc agaattcatt
     8641 gttcacttcg tgcggatagt cactagaaaa aacgaacggc ttatttccta cccgctcgat
     8701 cgcataggcg atatcgggct cttcgccttc gcagccgata aaaatccggc cttcgtcgat
     8761 gtgtcgcgag atatattcac ggactttttc tttggggcgt agattgagaa aacgtttgcg
     8821 gggatcatgc tggatgtgtg tctcccacga gcgatcgaag cgttccaagc acatcagcag
     8881 ccaagcgacg cctccttcca taaaaccaaa tttcacatta ggaaatttat cgaagatgcc
     8941 gttgaagacc atgcctgcaa atgaaatcat ctgaccaaag ggatgaccca gagcgtgaac
     9001 cggcgcatac gggctcaaat catccagccc catgttttca tgggcaccgc cgtgaacgcc
     9061 gagtgcgcaa cccaaacgat cggcttcttc atagatcggc caataacgct gatcaccgag
     9121 attcgattga ataccggtgg acggcagcat cgcgccgcac atgcccagtt ccttcaccgc
     9181 ccgtctcagc tcgtcaacag cggcctgcgt ttcttggagc ggaatcaaag caattccctt
     9241 gaagcgtgga cttttcttca ggtactcatg gtacaaccag tcgttgtagc cgcgcgccac
     9301 gtcgattgcc caatcgcggc tgacaatttt cccgaacgcc aggccaaggg tggtgtaaag
     9361 cacggttgtc tcaattccca catcctccag gaaatccaac caaccttccg gaccgacttt
     9421 gttgaatgct cccggctgga aatcgtgcat gttggctgaa tggagatgat ccagcggtgg
     9481 aaacggattg aagaatggat gggtatcgaa cttatcacgg tagggttgag gcatgaaccc
     9541 aaccatagac ttggtatcct cgatcacgtg accatcgcca tcaatgatcg tataaccttt
     9601 aaatcccata acgaatcctc ctacagcgta tttgagcact cagcgcggcc caattgtcaa
     9661 gaacaaccgc ccggttttct gcggccagca acgatcacgg ccctgcaagt cgagtgggcg
     9721 gtcactttgt aattatcgct gctctgactt ctggtgctca tccgaacaaa ataattgcct
     9781 acaaattgcc tcttgagccg gctactgcag ggttgcagtt ataatcaatt cgctttcgct
     9841 gctgctggcg cgctcgttgt atcctaggat ctgccgggtg agttcgtgta tgcggctcgt
     9901 tgaaccgcct agcgacacag gttggccatt gggaacgatc atctcagtca ttgcctcggt
     9961 gaaatcgatg gcgccggaat gctcggcaga aaaataactt atccgaggcg ttagtcggac
    10021 ccgtatctgc ttgtctgcaa gagcgttggc agccacacgc aacgaagttc caaccgcggt
    10081 gaaagtaatt cgccgatcga tggtgccgat ccggccggcg taatcacgat agaagcttac
    10141 ctggttctgc ggcacactcg ttgccaccgt taaaatcgat tccccaccat cttgcaccaa
    10201 ggtaaaaatt ccgctggatc gctaaaccgt tgtctgccgg ttgccggccg tcacccgggc
    10261 ggagggttgc gcgccgcctt ggcggattat gatgctgccg cttccttgca cctcttcctg
    10321 attgcctgtc actcgttgct ggctatcgac cagcactttg atgtgccggg atgtctgcgc
    10381 gccggcagga gcgagaacga gcagcgcgaa agtaaaatat agtacgatgt tcagtaaagt
    10441 ggattttttt cgcagcatgg acgttcgtga tctgtagtgt gttgaaaaac ttgtttgaag
    10501 gctgctcaaa agacctcaga tgcaaggcgc gcgaatttcg atgagcggag gcgtacgctt
    10561 tgtacgttga agcgagacga tcgagcgcaa cgaagcatat gagtcttttc agcagccttc
    10621 tagcgattac ccggcagcag agagaagtca agcactttct gcgggtcgaa atctgccgca
    10681 atcggccgct cctgtcgtaa cagctccaaa tagccgcgtg ccactttagg atctaatcgt
    10741 ccgtccttat taagaatctt gagcaatgta ttatatgtct gctcggcctc catggcagtg
    10801 actttaaatt tatcggcaat catcttgctg ctttcggtgc ggttggcgcg aatccaaccg
    10861 acagaatcgc ttagcgcggc aagcgttctg cgcacggtct cgcggttgtt tttaacttgc
    10921 gcctgcgaag taaccacgcc gacgtaagga atgtcggcca aatcgcccag aaacatgaat
    10981 ttcttgtagc caagagagac cgccttgtca tcgaacggcg gcgtcagcac cgtgccggcg
    11041 acccgattgc tggccagggc cataaagcta tcggcagtgc cgcccgtgct gataatgccg
    11101 atgtccttct cggcgatctc ccattttttc agagccgcct gcaaaaagta atgcggcgat
    11161 gttcgaaggc ctgaaaccgc gacttgcttg ccgatgagat cttgcggctt gttgatatcc
    11221 ttctgcgtga ccaagatcca cggcgcgcca tcgcaaaaat tcaagaccac catcaagggc
    11281 aagccggaaa ccgccgccga aatgccggtc gagaccgatg gaaaaaagtt gatgttgccg
    11341 ttgatgacgg cttgaggctg aatgcctgtg cgcatctgga tgtactcggc ttccaagcca
    11401 tattttttca aaaccccttt ttcctgggcg acgtagtacg gcatgtagga gaacgccagc
    11461 gaaggaaaac cgactctgat cttctgcagg ccttcggcag cacgaacact gctgctcagg
    11521 aacagaacga aagccaggaa cacgaacacg agagggttgc ttgctttggg cggggatttc
    11581 cggttcatcg aagctccctg ggaatcacgc cgtggcgatc ttttgctggc ctatgcttgc
    11641 ccagctttca cgggctttct taagcgcagc gggttgcggc aggttcttca gtttgaagaa
    11701 ctcgatcgcg ttgcggttta acaatctttc cttttcagga aaagttaaat cctccccatc
    11761 gacgacgtcc ttcaccgaat agggaaagtg acaatcccaa tgacagtagt ccgaagcgta
    11821 gagaaccgta ttttcgccgt tagccgcgaa cacccgatcg aggccggtct cttcggattc
    11881 gcaggtgagc acgacttgtt cgccgcggat aatttcg
//
LOCUS       DASUVS010000262        10534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1418327, whole genome shotgun sequence.
ACCESSION   DASUVS010000262 DASUVS010000000
VERSION     DASUVS010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10534
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418327"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VF182_14375"
     CDS             <1..487
                     /locus_tag="VF182_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438322.1"
                     /translation="KVARRKKHMDKRKILWSASLIVALMNVPLASAQDDRRDDRHYDR
                     RDDERHDRRDDRRGNWWDGIINGSAASSRDTDRRDLEGTWYLNGDRDKRTQITSSRDG
                     LEAKNENGDSSRLEISRNGDVYARDWERGLRGNVRRDRIEWDNGTTWTREPSGREASR
                     R"
     gene            complement(908..1939)
                     /locus_tag="VF182_14380"
     CDS             complement(908..1939)
                     /locus_tag="VF182_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438323.1"
                     /translation="MRKSSILGMLLAAFLSFFTLVSPSYGATVAYKFITQDIPIQFTF
                     LQETQEDIVRFTDINKKGEIIGNSFAGNGFLIKNKHTAMEIHCPGDQSDHHSTFVAGI
                     NNFGQVVGSCTDGAFVRDRNGDITMLNFPGAEGTVASAINDMGHVVGQYWGTLFGQAL
                     ERFHGFLWKDGEYITIDAAFPDAMFTSLSGINNAGQIIGTYLHQRSGSGDPNDYDGEI
                     AFLYDNGSFTRLDSPDARAPFDCCGATTLPMDINNAGQILGSTYDGNGNLRFFLYDDG
                     NYSVITGLPNNVTDFSSAWGLNDQSEIAGSYTQRVACQTCSFGGEPGYRFISHSFVAE
                     PQRSHKKTK"
     gene            complement(2264..2349)
                     /locus_tag="VF182_14385"
     tRNA            complement(2264..2349)
                     /locus_tag="VF182_14385"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2314..2316),aa:Leu,seq:caa)
     gene            complement(2785..3813)
                     /locus_tag="VF182_14390"
     CDS             complement(2785..3813)
                     /locus_tag="VF182_14390"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX6438324.1"
                     /translation="MAKMNARMMRGKMALEVAAELYTRHMFSLGNVVNISIAPSSKEE
                     QHPAIRMGINGDLMGGMRAPIEVANRHMDIGFVNPSAIVTMAFRGKGFYEEKLPLRAL
                     ASFPSWDKIAFAVSSDLKLKSLADVRERKIPLKVSTRSSGVNNTTHYTISKILEMHGI
                     SLANIKRWGGTVHECPRPTSAERTAGIKTGAVNAVFDEGLHNWVPDALEHGFEILSIE
                     PKMLRQLELLGYKRSTIPAGKYPGLDEDVSTIDFSGWPLITHRWLPNEVAYAICEAID
                     ARQNVIPVDHERPLDMGMLCRSTEAAPLGIPLHPGATQYYREKGYLERDQVAAAPHKR
                     YDDRDDDI"
     gene            complement(3845..4927)
                     /locus_tag="VF182_14395"
     CDS             complement(3845..4927)
                     /locus_tag="VF182_14395"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6438325.1"
                     /translation="MAHYAGEVGLQFTTHIANQYTVPRWIELAELARSHKFDQVWVND
                     NLGYRNVFVILTAIASRVPIKLGTAILVPYFRNPVDTADAFAALSELVEGREISVGIA
                     RGDYAQAGHQINLLKPISFVKETVDCIKRLLQGETIYYKDYPALLSFYQLKEDASMHL
                     RFAPKAPILFYSGGNGPKIMRIAGQLMDGVLIGGFFIPLVRSGKLQPLLQAADIGRTE
                     SNREAGLKRVCEINISVSSDRERARVFPRRYIAHMLLVLEALGFSDQEFNALNIEPEP
                     IEKIKAAFVRGGTIEDVAPWITDAMLDAGFIAGTPRDCIEKLEEMCVHAQAYGFDQIC
                     LAKLGPDYEEAITLLSRDILPSIVQH"
     gene            complement(5540..6901)
                     /gene="radA"
                     /locus_tag="VF182_14400"
     CDS             complement(5540..6901)
                     /gene="radA"
                     /locus_tag="VF182_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX6438326.1"
                     /translation="MAKPKTFYACQSCGYQASKWLGKCPDCNQWNTFAEERLEKASSV
                     RGELSLGTREDPAPIHEINTAEEGRLQCGIGEFDRVLGGGLVSGSVILIGGDPGIGKS
                     TLLLQAFAAISQKGLTCLYVSGEESPRQIKMRAERLGIVAPNLLVLSETSLERIIEHV
                     KHIKPGLLVIDSIQTIFSPTIPSAPGSIAQVRESSGSIIVLAKKTGLTTFLIGHVTKD
                     GAIAGPRLLEHMVDTVLYFEGERGHPFRVLRAVKNRFGSTNEIGVFEMKEAGLKEVSN
                     PSEIFLMERPLQVPGSAVVCSIEGTRPILVELQALVSRSFLAVPRRTTIGVDHNRVAL
                     LVAVLEKKMGAKLFDQDIFVNVAGGVHVDEPAVDLGIIAAIASSSRENVLDPKTVFFG
                     EVGLAGEIRGISQAETRIKEAGKLGFDRCILPASNSAQLKHIKHPKLSGVSSLNECWK
                     MMF"
     gene            7075..7728
                     /locus_tag="VF182_14405"
     CDS             7075..7728
                     /locus_tag="VF182_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717365.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX6438327.1"
                     /translation="MVIERAENTSAADFLPRSRSLDSLRQAAKSCKGCDLYLNATQTV
                     FGDGPGHASVMLVGEQPGDIEDQKGEPFVGPAGRMLDRALEDARICRDEVYVTNAVKH
                     FKWIWRGKRRLHQKPSVRQVVACRPWLQVEIEAVQPHILVCMGATAAQSVVGKPVPIM
                     KERGKFIDSVFGKLTFITIHPSAILRQRDSDEREQEFRRFASEMKLIKRKLQSLSAA"
     gene            7822..8976
                     /gene="bioF"
                     /locus_tag="VF182_14410"
     CDS             7822..8976
                     /gene="bioF"
                     /locus_tag="VF182_14410"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX6438328.1"
                     /translation="MTAGFIQDELQALKSASLYRQLRHVAGDQGPTLMVDGREVLNFS
                     SNNYLGLANHPALGKAAIDAIERYGCGSGASRLISGNMTLHEELEVKLARFKGTQAAL
                     VFNSGFQANTGIIPTLVGEGDVVFSDALNHASIIDGCRLSRAKIVVYRHCDVDQLEDG
                     LKDAPHKARKLIVTESLFSMDGDEAPLKQIVELSEKYDAMTMVDEAHATGVYEPDGAG
                     LISQLRLGERVLVQMGTLGKALGGFGAYIAGSTALRELLINRCRSFIFTTSLPPAVMA
                     MAIAAIELIGREPQRRRALRNNCARVRDGLARSGYSLGDSRSQILPLMIGDANGCMRL
                     SERLLEHGVFAQGIRPPTVPQGTSRLRITLMATHTSDQIDRALEVFASVH"
     gene            8989..10347
                     /gene="bioA"
                     /locus_tag="VF182_14415"
     CDS             8989..10347
                     /gene="bioA"
                     /locus_tag="VF182_14415"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEX6438329.1"
                     /translation="MTQDYDRLKQLDHSYLWHPFTQMQEWIGEEPCIISRAEGHYLID
                     VNGRRYFDGVSSLWCNVHGHRKRELDDAIRDQLDRISHSTFLGLSHVPGIELAQKLIE
                     VAPPGLQRVFYSDSGATAVEVALKMSVQYWQLKHEAKRTLIATLAESYHGDTIGSMSM
                     GYSETFHRYHKSLLFPVLRITPPHVFRYYQRMSEAEALNAAADEAERKINENRTTLAA
                     LVVEPLMQGAAGMWVQPVEYLQALDKICRQYGILLILDEVATGFGRTGKMFACDHARI
                     TPDILCLAKGISGGYLPLAATLTSEEIFTAFLGDYKDFKTLFHGHTYTGNPLGCAAAL
                     ANLDLFRRENIIDNMQPRISYLQNRFEEEFVSMPHVSDVRQWGYMIGIELVEDKAGRI
                     SYPPEKRIGHRVIMEARKRNLMIRPLADIIVLMPPLSITDAELALLLDTVRDSIRAVT
                     GN"
     gene            10347..>10534
                     /locus_tag="VF182_14420"
     CDS             10347..>10534
                     /locus_tag="VF182_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX6438330.1"
                     /translation="MGKGVFITGTDTGVGKTFFACKLSALLRHSGYHVGVMKPAESGC
                     AERQGKLFPEDAIRLKEAS"
BASE COUNT         2452 a         2755 c         2805 g         2522 t
ORIGIN      
        1 caaggttgca aggaggaaaa agcatatgga caaaagaaaa atactatggt ctgccagttt
       61 gatagtagca ttgatgaacg ttccacttgc cagcgcccaa gacgatagaa gagatgatcg
      121 gcactacgat cgaagagatg acgaaagaca tgatcggcga gatgatcgtc ggggcaactg
      181 gtgggacggc ataattaacg gcagtgcagc aagttcgagg gacaccgatc gccgcgacct
      241 ggagggcact tggtacctaa acggggatcg cgacaagcgc acccaaatta cttcttctcg
      301 cgacggcctc gaagcgaaaa acgagaacgg cgattccagc cggttggaaa ttagtcggaa
      361 tggcgatgtt tatgcccgcg attgggaacg cggtctgcgc ggaaacgttc gacgcgatag
      421 aatcgagtgg gacaatggaa ccacctggac gcgggagcct tccggtagag aggcgtcgcg
      481 acgttaacaa gcaatcttaa aataatattt aaaaaacgaa atgcgcgggg cggactaacg
      541 agtccgcccc gcgcgttatt gctacacctg tttcccaccc cgcctcacct gcgaagcacg
      601 aaagcatcaa cctactttca actagaatag aggtctgtct tcgattccac gagtaaccga
      661 aaagaagaca gcgacgcgct cgtatatcac ccaaaaaatc atacctgtca ctgccagaat
      721 acctatcgtc accagaaccg gagtgacgat tcgaaggacc ggtgcagtag aatcatcggc
      781 aaagctgcgc agcttccgaa gcaggtcttg cgtatgccgc acaaatataa agatgctgcc
      841 atgatgagcg acattcgtaa aaactctaaa atctaacgaa tgttttctgt gcatcttctg
      901 cgattcctta ttttgttttt ttatgagatc gctggggctc agcgacgaaa ctgtgcgaaa
      961 tgaagcgata ccctggttca cctccaaagc tgcaggtttg gcaagccact ctctgagtgt
     1021 agcttcccgc gatttcgctc tgatcgttta gtccccatgc gctgctgaag tctgtaacat
     1081 tattgggcag gccggtaatc acggagtaat ttccatcatc gtaaagaaag aaccgcaagt
     1141 ttccattacc atcgtacgtg gacccaagta tttgaccggc gttgttgatg tccatcggca
     1201 gcgtcgtcgc tccgcaacag tcgaacggtg ctctggcgtc cggggaatcg agccgagtga
     1261 agctgccgtt gtcgtataga aaggctatct ccccgtcata gtcgtttgga tcaccgcttc
     1321 cggatcgctg atgtaagtac gtgccgatga tctgtccggc attgttgatg cccgacaaag
     1381 atgtgaacat cgcatccgga aacgcagcat cgatggtaat gtactctccg tccttccaca
     1441 gaaacccgtg aaatctttct aaggcttgcc cgaaaagagt tccccaatac tgacctacga
     1501 catggcccat gtcgtttatt gcggaagcga ccgtgccctc agcgcctggg aaattaagca
     1561 ttgtgatatc gccgttccta tcgcgtacga aggcgccatc ggtacaggag ccaacgactt
     1621 gtccgaaatt gttgatgccg gcgacgaatg tgctgtgatg gtcggattgg tcgccggggc
     1681 agtggatctc cattgctgtg tgcttgttct taattaaaaa gccatttccg gcaaagctgt
     1741 ttccgattat ttctcctttc ttattgatgt cggtgaagcg aacaatatct tcctgagtct
     1801 cctgcagaaa tgtaaattga atgggtatgt cctgagtaat gaacttgtac gcgacagtcg
     1861 cgccgtagct gggactcact agagtaaaaa acgacaaaaa ggcggcgagg agcatcccaa
     1921 gtatcgacga ttttctcata gtcccctcct ttgatggttt ggcatgtatt ggcatatttt
     1981 gtcatttttt gtcaattatt ttctccgttg atttaaactt gtgctctggt ggcttatctg
     2041 cctgctaggc gacgtgtagc ttcgccttgc ctgagggcgc ataagacgag actctttgat
     2101 cgctccaata ttgttagcga catgcggatt ccgagtacgc gggagtgagc gcaggatgcc
     2161 agggaaagta tacctttgat gcgggctggg ggcagtaaac agagagctat tcgaaagaag
     2221 agaaggaaag gaaagtaggt gcggtattta gctataaaaa acttggtgcc ggaggtggga
     2281 cttgaaccca cacagggttg cccctaccgg attttgagtc cggcgcgtct gccattccac
     2341 cactccggca cagtaatggt ttcaataact tataagtctt tcggtttcaa gttgctgtgt
     2401 cgggaagaac tttttaatgc catttagaag atttccgcgt ttaaacctag catgggaacc
     2461 gcaacgatca gatagactgt tgcgtctaga catctgccgc tcttcgtgcg agagcttttt
     2521 cccaatcctc acggaaacca atatcgttcc gggcgatctc ctgatacctc gtggagaggc
     2581 gagtctcttc ggcgactgcc ggcggggcat gagaatgaga ctctccctat gagggctttg
     2641 atatagaatg tcggtactac cgggtcaaga aatgatcgga agtttagtcg caagtcttct
     2701 tgtcgccacg tcagtttttg gttcgctgcg cgcggacaat gatccaccgc cgatgttatc
     2761 acagctttat tcctcgacag ctactcatat gtcatcgtcc ctatcatcgt atcttttgtg
     2821 gggggcagcc gctacttgat cccgttcgag gtagcccttt tctctataat attgggtggc
     2881 tccggggtga agcggaattc ccaagggcgc agcttccgtg gatcggcaga gcatccccat
     2941 gtcgagcgga cgctcatgat cgacaggtat gacgttctgc cgcgcgtcta tcgcctcaca
     3001 gatcgcatag gctacctcgt ttggcagcca acgatgcgtg atcaacggcc agccactgaa
     3061 gtcgattgtg gagacgtctt cgtccaagcc cgggtatttt cctgcgggaa tcgtcgatct
     3121 cttataaccc agcagctcta attgccttaa catcttcggt tcaatcgaaa gaatttcgaa
     3181 tccgtgctcg agcgcatcgg gaacccagtt gtggagccct tcgtcgaaca cagcatttac
     3241 agctcctgtt tttatgcccg cagttcgttc cgccgacgta ggccggggac attcatgaac
     3301 ggtcccgccc cagcgtttga tgttcgcgag cgagatgcca tgcatctcca gaattttgga
     3361 tatggtataa tgcgtcgtat tgttcacgcc ggacgatctc gtcgagactt tcaaaggaat
     3421 tttcctttca cgcacatcag cgagagactt caacttgaga tcactagaca cggcaaaggc
     3481 gattttgtcc catgacggaa aggatgccaa agctcggagc ggcaatttct cctcgtaaaa
     3541 tcccttgccg cgaaaggcca tcgtcacgat ggcggatggg ttgacgaacc cgatatccat
     3601 gtgtctgttg gccacttcga tgggcgcccg catccctccc atgagatcac cgttgattcc
     3661 cattctgatc gccggatgct gttcttcttt tgagctgggg gcaatggata tgttgacgac
     3721 gttgcccagg gaaaacatgt gcctggtgta gagttctgcc gcgacttcga gggccatctt
     3781 tccccgcatc atccgggcgt tcattttggc cattttggct gtcgaccttt cttccgcggc
     3841 ccgttcaatg ctgaacaatt gacgggagta tgtcgcgcga gagtagcgtg atcgcttcct
     3901 cgtaatcggg cccgagcttg gcgagacaaa tctgatcgaa accgtaggcc tgggcgtgga
     3961 cgcacatttc ttccagtttt tcgatacagt cccgaggagt gccggcaatg aaaccggcat
     4021 caagcatcgc atcggtgatc catggcgcaa cgtcttcaat cgtgccgcct ctcacgaacg
     4081 ctgctttaat tttttctatt ggttcaggct cgatattgag cgcgttgaat tcttgatctg
     4141 aaaaacccag cgcctcgagc acaagcagca tgtgggctat gtaacggcgc ggaaaaactc
     4201 tcgcccgttc gcggtcactc gaaacagaga tattaatctc gcagactctt tttaggccgg
     4261 cttcccggtt cgattcggtc ctcccaatat cggctgcctg aagcagcggt tgcaatttgc
     4321 cgcttctgac aagtggaata aaaaaaccgc caatcagaac tccatccatg agctgacccg
     4381 cgattctcat aatcttgggt ccattgccgc cgctgtaaaa caaaatcggc gctttgggag
     4441 cgaagcgcag atgcatgctc gcgtcttcct tcagttggta aaaggataga agagccggat
     4501 agtccttgta gtaaatcgtc tctccctgca atagacgttt gatgcaatcc acagtctctt
     4561 tgacaaagct gatcggcttc aacagattta tctggtgccc ggcctgagcg tagtcacctc
     4621 gcgctatgcc gacgctgatc tccctcccct caacaagttc cgagagtgcg gcaaaggcat
     4681 ctgcagtgtc gacaggattt ctgaagtagg gcacgagaat cgcggttccg agcttgatcg
     4741 gcacccgtga tgctatcgcc gtcaaaatga caaagacgtt tctatatccc agattgtcat
     4801 tcacccagac ctgatcaaat ttgtggctgc gggcaagctc tgcaagttcg atccaccggg
     4861 gaacggtata ctgattagcg atatgggttg tgaactgtaa cccgacctcg ccggcatagt
     4921 gcgccattta acctcagttg tgttgtgtag tccaagcaga aaatatgcgt ttcttatttc
     4981 atctttcaaa aaagaaaaaa agagctccgg tttcagctgc gggacgccac gaaattgcgc
     5041 tctcgccaac acggtgttga accgtcatct attcaggagc gcaggatggc tttttcggct
     5101 cactcttttc gggaaatact agggatacga agctccttca gagtatcaac tattctaagc
     5161 tcacttcccg ctgcttttaa aatcggtttt aaaatttttc ccgtgtaaaa gtcagaacta
     5221 gagaaatagc aaagtaggaa aatgcgcggc ctactaattt tgcgagagag gctgaagctg
     5281 tttgaaaatg ctctacggcc acttcgaaac ttaccttctt ttgagtccgg cgcgtctgcc
     5341 attccaccac tccggcacgg cggtattagt aataaccaag catcgtttcg atacaagagg
     5401 acgatgtgaa aggatgaaag ctgaaagcgg aaatttaaaa tgatcgcatg tttctctttt
     5461 ccgatcccca taccagcgga tcgctcattt ggattggcgc tggatcactt tatcatcgac
     5521 gaccttgata aaccaaaatc tagaacatca tcttccaaca ctcgtttaga gagcttaccc
     5581 cggagagttt gggatgtttg atatgtttga gttgcgcact gttgctggcg ggaagaatgc
     5641 accggtcaaa gccgagcttg ccggcttcct tgatgcgggt ttctgcttga gatatccccc
     5701 gaatctcacc ggccaggccg acttctccga aaaaaactgt tttcggatcg agaacgttct
     5761 cgcgactgct cgaagcgatc gcagcgatga ttccgagatc gaccgccggc tcgtcgacgt
     5821 gaacgccgcc tgcaacgttg acaaaaatat cctgatcaaa gagtttggcg cccatcttct
     5881 tttcaagaac cgccaccagc aaagcgaccc gattgtgatc cacgccgatg gttgtccgcc
     5941 ggggcacggc caggaacgat cgactcacca gcgcctggag ctctactagg ataggccgtg
     6001 tgccttcgat gctgcagacc accgctgatc ccgggacctg aagcgggcgc tccatcagaa
     6061 aaatttcgga aggattgctt acctctttca gccctgcttc tttcatttca aatacgccga
     6121 tttcattggt cgagccgaag cgatttttga ccgcgcgcaa cacgcgaaag ggatggccgc
     6181 gctcaccctc gaaatagagc acggtatcga ccatgtgctc gagtaggcgc gggccggcga
     6241 tggcgccgtc tttggtgaca tggccgatca agaaagtcgt tagaccggtc tttttcgcca
     6301 gcacgatgat tgagccggag ctttctctca cctgagctat gctgccggga gccgacggga
     6361 tggtcggact gaaaatggtt tggatcgaat cgatgacgag cagcccgggt ttgatgtgct
     6421 tgacatgctc tatgattctc tccaaagagg tttcactgag aacgagcagg ttgggggcga
     6481 cgatgcccaa gcgctcggcg cgcattttga tttgccgcgg cgattcctcg cccgatacat
     6541 aaaggcaggt gagtcctttc tggcttatgg cggcaaacgc ctgcaacagc aacgtcgatt
     6601 tgccgatgcc gggatcaccg ccgatgagaa taaccgatcc agaaaccaga ccacccccca
     6661 atacgcggtc gaactcgccg attccacatt gcagccgacc ttcttccgcc gtgttgattt
     6721 catgaatcgg cgcgggatct tctcgggtcc ccaggctgag ctcaccgcgt acactgctgg
     6781 ctttttcaag tctctcttct gcgaacgtgt tccattggtt gcaatcgggg catttgccca
     6841 gccacttgga cgcttgatag ccgcagctct ggcaggcgta aaatgttttg ggtttggcca
     6901 tttatctttc tgtcctcaaa ttgtcagaag agtttaatcc agtgcgagcg aaagtagcaa
     6961 atcaagccat gctgctatca caaatacagg cgcaatcatc gccgtccatg gtgcgttggc
     7021 ggccttgcga gtcggctatc tagactcaaa ccgcgcaagt gataggcttt caacgtggta
     7081 attgaaagag ctgaaaatac ttcggccgcc gatttcttgc ccaggagtcg ttcgcttgat
     7141 tctctccggc aggcggcaaa atcgtgcaag ggatgtgatc tttacctcaa tgctacccag
     7201 acggtctttg gcgacgggcc tggccatgcg agtgtgatgc tggtgggtga gcaacctggc
     7261 gatatcgagg atcaaaaagg tgagcccttc gtcggtccag ccggccgcat gcttgaccga
     7321 gcgctggaag acgcacgtat ttgccgcgat gaagtgtacg tcaccaacgc ggtgaagcac
     7381 ttcaaatgga tctggcgtgg caagcggcgc ctccatcaga aaccctcggt gcgccaggtt
     7441 gttgcatgca gaccatggct gcaagtcgag attgaagcgg tgcagcctca tattcttgtc
     7501 tgcatggggg ctacggccgc acagtcggtg gtgggtaaac ccgtaccaat catgaaagag
     7561 cgcggcaaat tcatcgattc cgtgttcggc aaactcacct ttattacgat tcatccatct
     7621 gccattctcc gccagcgcga tagcgatgag cgagaacaag aattccgtcg ttttgcttcc
     7681 gagatgaaac tgattaagcg caagctacag agcttgtcgg ctgcgtgagc ttttctacgc
     7741 gatcgatcat tctcgtgcca aaccgttgat cacgatctcg tcttgccacg gcctcacctg
     7801 tgtattatta gttcacagca aatgaccgcc ggattcatcc aagatgaact gcaagctctc
     7861 aaatcggcat cgctctatcg tcagctgcgg catgtcgcag gcgatcaggg cccgactttg
     7921 atggtcgacg ggcgcgaagt attaaatttc tcgtccaaca actatttagg attggccaat
     7981 cacccggcct tgggaaaagc cgcgatcgat gccattgagc gttacggctg cggttccggc
     8041 gcgtcgcgtc tgatctccgg caatatgacc ttgcatgaag agctcgaggt taagcttgcc
     8101 cgattcaagg ggactcaggc ggcactggtt tttaattccg gcttccaggc caatacaggg
     8161 attattccga cgctggtcgg cgagggcgac gtggttttca gcgatgcgct caatcacgcc
     8221 agcatcatcg atggttgccg gctatcgcgg gcgaaaatcg ttgtgtatag acactgcgat
     8281 gtcgatcaac tcgaagacgg cctgaaagat gcgccacaca aagcgcgcaa gttgattgtc
     8341 accgagtcgc ttttcagcat ggatggtgat gaagcgccgc tgaaacaaat tgtcgagctt
     8401 tctgaaaaat atgatgccat gactatggtg gatgaggcgc atgccacggg cgtgtacgaa
     8461 ccggacggcg ccggcctcat ttcacaactg cgtctaggcg agcgcgtgct cgtgcaaatg
     8521 gggacgctcg ggaaagcgct gggagggttc ggcgcctata tcgccggcag cacggcgctg
     8581 cgcgagttgc tgatcaatcg ttgccggagt tttatcttta caacatcact gccgcccgct
     8641 gtgatggcga tggcgattgc cgctatcgag ttgatcgggc gtgagccgca aaggcggcgc
     8701 gctttgcgca ataactgcgc gcgagttcgc gacggattgg cgagatcggg atactcccta
     8761 ggtgacagcc ggagccagat cttgccgctg atgatcggcg atgccaatgg ctgtatgcgc
     8821 ctttcggagc gtttgctcga gcatggtgtg ttcgctcaag gcatccgtcc acccaccgtg
     8881 ccgcagggga cgtcgcggtt gcggatcacg cttatggcga cgcatacatc cgatcagata
     8941 gatcgagcgc tcgaggtatt cgcatcggtt cattagcgat cggccggaat gactcaagac
     9001 tacgatagac tcaagcagct ggatcatagc tacctctggc accccttcac gcaaatgcag
     9061 gaatggatcg gcgaggagcc ctgcattatc agccgtgccg aggggcatta tctgatagat
     9121 gttaacggcc gcagatattt cgatggagtt tcatcgcttt ggtgcaacgt tcacgggcac
     9181 cgaaagagag agctcgatga cgcgatacgc gaccagctcg accgcatctc gcattcgaca
     9241 ttcctcggcc ttagccatgt gcccggcatt gagttagcgc aaaagctgat cgaagtggct
     9301 cctccaggct tgcagcgggt tttctattcc gacagcggcg ccacggcggt cgaagtggct
     9361 ctcaaaatgt cggtccagta ctggcaattg aaacacgaag caaagcgcac cctgatcgcg
     9421 actctagcgg agtcgtacca tggcgatact attggttcga tgagcatggg ctattctgaa
     9481 acctttcatc gttatcacaa gagcttgttg tttccggtct tgcgcatcac gccgccgcat
     9541 gtctttcgct attaccagag gatgagcgaa gccgaggcgc taaacgcagc tgccgatgaa
     9601 gcggagagaa aaattaacga gaatagaact acgctggccg ctctggtcgt ggagccgctt
     9661 atgcaaggag ccgcgggtat gtgggtccag cctgtggaat acctccaagc tctcgataaa
     9721 atctgccggc aatatggaat tctattgatt ctcgatgaag tagcgaccgg gtttggccgc
     9781 accggaaaaa tgttcgcatg cgatcacgcc cgcataaccc ccgacatcct ctgtctggcc
     9841 aaaggcatca gcggcggcta cctgccattg gcagcgactc taaccagtga agaaatcttt
     9901 accgccttcc tgggtgacta caaagatttc aaaaccttat ttcacggcca tacttacacg
     9961 ggaaacccgc tcggctgcgc cgcggcgctt gcaaatcttg acctattccg gagagaaaac
    10021 atcatcgaca acatgcagcc gagaatctca tatctccaga atcggtttga agaagaattt
    10081 gtttcaatgc cgcacgtcag cgatgtacgc cagtggggtt acatgatcgg catcgagctg
    10141 gtcgaagata aagcgggccg tataagttat ccgcctgaga agcgcatcgg ccatagagtg
    10201 atcatggaag cgcgcaaacg caacctcatg atccgccccc tggctgacat catcgtactg
    10261 atgccgccgc tgtcaattac ggatgccgag ctcgcgttac ttttggatac cgttcgtgac
    10321 tctattcgcg ctgtgacggg aaactgatgg gaaagggagt tttcattacc ggcacggaca
    10381 ccggcgtcgg caaaaccttc ttcgcgtgca agctgagcgc gcttctgcgt cactccggct
    10441 accacgtcgg cgtgatgaag ccggcggaga gcggctgcgc tgaacgacaa ggtaaacttt
    10501 ttcccgaaga cgcgattcgt ttaaaagagg cgtc
//
LOCUS       DASUVS010000263         8832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_763136, whole genome shotgun sequence.
ACCESSION   DASUVS010000263 DASUVS010000000
VERSION     DASUVS010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8832
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763136"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(774..848)
                     /locus_tag="VF182_14425"
     tRNA            complement(774..848)
                     /locus_tag="VF182_14425"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(813..815),aa:Glu,seq:ctc)
     gene            complement(851..926)
                     /locus_tag="VF182_14430"
     tRNA            complement(851..926)
                     /locus_tag="VF182_14430"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(891..893),aa:Gln,seq:ctg)
     gene            complement(986..2410)
                     /gene="gltX"
                     /locus_tag="VF182_14435"
     CDS             complement(986..2410)
                     /gene="gltX"
                     /locus_tag="VF182_14435"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX6438331.1"
                     /translation="MTQIRTRFAPSPTGYLHIGGARTALFNYLFARHHYGKFILRIED
                     TDRERSTHEAINAILEAMNWLKLDYDEGPFYQKDRFPLYKDKIEQLLATGKAYPCVCT
                     PQDLDAKRQLAQKEKRKPSYDGTCRPAEGVIPRIPEDKPFTIRFRSPQLGTTVVNDLV
                     KGNVIFDNRELDDLILARTDGTPTYNFCVVVDDIEMKITHIIRGDDHLANTPRQIQLY
                     HAMGHTLPQFAHVPLILGTDKARLSKRHGATSVTAYRDMGYLPEAVVNYLVRLGWSHG
                     DDEIFSREELIEKFSLESIGKSAGVFNPEKFLWLNSHYIKSRPLRQLAHDIVPFIQAR
                     GYPVPKNLDWLEKMAATLKDRAKTLVELVEQAHYYLSDDISIDEKAVKKFLIPEIREP
                     LNSLIRKLSDLADFSDVAVQQAFTEVLEQTGLSMGKLAQPVRVALTGSTVSPGIHEVI
                     AVLGKERTLKRLNTAVERLAFQGQ"
     gene            2539..4239
                     /locus_tag="VF182_14440"
     CDS             2539..4239
                     /locus_tag="VF182_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEX6438332.1"
                     /translation="MAPAEGAPSNFIRTIVADDLETGKHHGRVHTRFPPEPNGYLHIG
                     HAKSICLNFGLATEFGGLCNLRFDDTNPSKEDIEYVESIREDVRWLGFDWGDREYYAS
                     DYFDRLYQFAVQIIKAGKAYVCDLNSDQMREYRGTLTEPGRNSPFRDRSIEENLNLFE
                     RMKEGEFPDGARTLRAKIDMSSPNLNLRDPVMYRILHAAHHRTTDHWCIYPTYDFAHG
                     QSDSLEGITHSICTLEFEDHRPLYDWFLDQLGIHHPQQIEFARLNLSHTVLSKRKLLQ
                     LVQQNHVSGWDDPRMPTLAGFRRRGYTAEAIRTFCERIGVAKRNSTVDIAMLEHCLRE
                     DLNKRAPRAMAVLRPIKVVIENYRAGEVENLEAVNNPEDPSSGTRLVPFSREVYIEQD
                     DFREEPPKGFFRLSPGREVRLRYAYIIKCTDVVKDPRTGEIRELHCIYDPETKSGSSR
                     SNRKVKATIHWVSAAHAKEAEVRLYDHLLSKEDPDDVPDGVDWLTNVNPKSLEVLTEC
                     RVEPILVNAKSGERYQFERLGYFCVDSVDSTERKPVFNRTVTLRDSWANLQKAAKRSK
                     "
     gene            complement(4303..5607)
                     /locus_tag="VF182_14445"
     CDS             complement(4303..5607)
                     /locus_tag="VF182_14445"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX6438333.1"
                     /translation="MRNKFVKIAVMMVGPIFVFSLAHGVFAGGFMIPHQTGRALGLSN
                     AVTASVNDPSAVFYNPAALGEVHGNNLLVSGTYVGLVSSVENSGRRAVNKHDDNFLAT
                     LFGNYHLPNTDFSFGIGAYSPFGLATSYESDFTRFAAERTELKTLYITPAAAWHPSQY
                     FSIGAGASFVHASGMFSRGLCLDPITSCTSPIGLEGRLRLTDTTNAFAYNIGALIKPT
                     DTVKIGFSYRSRVDLRFDDADVKLGGSFSTATTKSDIRPIPLPPVINTGIFWQVRPNW
                     GAELVYEYTRWSEFQDFTATFSPSPTFLPLGFPISGFNFPEDWKNTSTLRFGSYYALN
                     KNWELRGGLAVEESPIPNHTLNPAIPGADLLTLNAGVGYKWENFSVDFGYMAVFYKDR
                     RVTNNELEGLPATSPLGFAGAPGKDKYETFNNFVTVSLGYRF"
     gene            5807..7546
                     /locus_tag="VF182_14450"
     CDS             5807..7546
                     /locus_tag="VF182_14450"
                     /inference="COORDINATES: protein motif:HMM:NF014343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6438334.1"
                     /translation="MSRQCKEILSGDDISLQANGGFTTEPLILTTASAKKIVAYLDLP
                     NSSESPLGTVLIAPAYGETKENNLLISAYLAANRFCGLRFDWSDHVGESEGDICTSTL
                     TKMRQVLVSLLDYAQLRFSEQPVGIFASSLAARVAIKVTAQDRRPAFLVCLTPVVDLR
                     ETLTMVYKEDLAGNYSAGKRYGTIDILGLTINADNFLEDAITNSFVDLMSTKRDARSI
                     AIPSYFVVGERDTWVRIEDAKSVVECLSSATKTIVVLSTMLHRLLESPTIAVSAIKEI
                     VRNLIECTDRRKANPHSIQIPDSNQIRFRKSHERNQLRERYEYSQAEERGFWKAYLSK
                     FRYVFNIPDFYALLESIHNRLGGVWAGQKVLDIGCGVGNFGLFLLTKQLYRAQQDLQY
                     AKLEPLRYYGVDFIWEAISAASRQISELRTDFMSRIGLASSNGSFLKEQFVLADLEVG
                     IPFPNEFFNNACCNLVISYLQDPATMLKELWRVLRPGGSVVISSLKPNADLSEIYRNF
                     ISFTDDRLEIEEGRHLLNNAGLIKVKEAAGIYHFYSEKELRDVVRKAGFHRAKTFRSF
                     GNQANLVVGYKGY"
     gene            7674..>8832
                     /locus_tag="VF182_14455"
     CDS             7674..>8832
                     /locus_tag="VF182_14455"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HEX6438335.1"
                     /translation="MKFYAISGLLNLVTSSVVGIFVFTRDVSDQRYQTYGAFCASLAV
                     WSFGYLFWHLSDTSQEALLWVKVLMAGAIFIPITNFHHIVSLLDKPPWEARWIFFGYW
                     VSFVFLYLDLFSPLFIKDVAPKSEFPFWPVPGPAFHAYLTLFIYCVFRFIYLLWKQIR
                     VNTGIKRNQFKYLVCALSLGYVGGGTNFLLWYDIPIRPYGNILVSVYVVLFSYAVIKF
                     SLLDIDVVIKKSLVYVLLLVVLIAPCFALVITAQALTFGEINYDFSLFTLCVFIVVGF
                     FFPKLRIHAEGALEKVLFKKKYDYRETLLRSSRDMISLIDVDSLSERLVRTISSAMRV
                     EKVSLCIADESKGEFRFTAFTGYAIGSFKHIALSKDDPFIQNFRRSSLTVVR"
BASE COUNT         2246 a         2037 c         2257 g         2292 t
ORIGIN      
        1 gtgaagtgaa tcacttgcgc ctctttcttt tgcggtgttt tcttgagcgg tgccgccatg
       61 ccaggaatcc gaggtaaagg atgcctagag ctaagacgat caggtctatc gattcgacag
      121 ttagcaccgt gtcagagata aaaccgagcg aaacgcctgt gattagaaat cactgatgag
      181 agaactaatt ctaataagag cgttgtccaa acgaaaacga gcagtgcctc ctcaccgtaa
      241 ttggaccaga agttccgcaa cttgattcct cgcgctaagt tctgaccgca agaacgatac
      301 cgatccaaaa tattctaaaa tatcgtttaa agggcgtata tggcaatatt tgaccgatgc
      361 gattcgttga caatgactga cacgtcacgc ccaggcaaac tcagcaacgc agtcgttatg
      421 cgagcaagtt gcaggggggt tagggaaagc cgagagtctc atgcgaatag agcaggaggc
      481 ccgcagtaac ggagatcttc attttaacta cagatcagcc cttggtaacg tttcgcgcga
      541 gcgcggaact tgcccgcgtg agctccgacc taagagtttg caaacgacat ggctcctaat
      601 gtgtttcgta ccggagttaa attattcagc tcgtcagggg tgaggaccaa gttgtaaatg
      661 ggcggcggaa gttgaccgaa gggtagctta gatgagagcg ccgtcagtta acgagtaaac
      721 gactttcttt gggctgaact ttaagcgctg gcatcattag tatgtgggaa cattggtggt
      781 cccaacggga atcgaacccg tgttaccgac gtgagaggcc ggtgtcctaa ccgctagacg
      841 atgggaccat tggctgagga ggaaggactc gaaccttcaa tcgcctgatc cagagtcagg
      901 tggcttacca attagccgac tcctcaactt cttagagggt tcaaattaaa agaatcaagc
      961 ggtgaaatca aggcagattg gtctgtcatt gcccttggaa cgccaagcgt tctaccgcgg
     1021 tgtttaatcg ttttagggtt cgttctttgc caagaaccgc gatgacttca tgaatgccgg
     1081 ggctgaccgt tgacccggtt agcgcgacac gtaccggttg ggctaacttc cccatcgaca
     1141 aacccgtttg ctcgaggact tccgtgaagg cctgctgaac agcgacatcg ctaaaatcag
     1201 caaggtcgct aagctttcgt ataagactgt tcagcggctc ccggatttct gggattaaga
     1261 atttttttac agccttctca tcgatcgaaa tatcatccga gaggtagtaa tgtgcttgct
     1321 cgacaagctc caccaaagtc ttcgctcgat ccttgagtgt tgcagccatt ttctcaagcc
     1381 aatccaaatt tttcggcacc gggtaacctc tggcctggat aaatggaacg atgtcatggg
     1441 ctaactgtcg cagcgggcgc gacttgatat aatgagagtt tagccagagg aatttttcgg
     1501 gattaaagac ccctgcagac ttaccgatac tctccagaga aaatttctca atgagttctt
     1561 cgcgagaaaa aatttcgtca tctccatgcg accaacctaa ccgaactaga tagttgacaa
     1621 ccgcctccgg gagataaccc atatcgcgat atgcggttac ggaagttgct ccatggcgtt
     1681 tgctgagacg cgctttatcc gtgccaagaa tcaaaggcac gtgggcaaat tgcggcaagg
     1741 tatgtcccat agcatggtac agttgaattt gccgtggtgt gttggccaga tgatcgtcgc
     1801 ctcggatgat atgggtgatc ttcatttcga tatcgtccac caccacgcaa aaattgtacg
     1861 tcggagttcc gtcagttcgt gcaaggatca aatcatcgag ctcgcggttg tcaaaaatca
     1921 cattgccttt aacaagatca ttgaccaccg tagtgccaag ctggggcgag cggaatcgga
     1981 ttgtaaaagg cttgtcttcc ggtatacgcg gaataacgcc ttcggccggg cggcaagtcc
     2041 catcataaga gggcttgcgc ttctcctttt gcgcgagctg acgtttggca tcgagatcct
     2101 gaggtgtgca cacgcaagga taagctttgc ctgttgcgag cagttgctcg attttgtcct
     2161 tgtagagcgg aaaccggtct ttctgataaa agggtccttc gtcgtagtca agcttcagcc
     2221 aattcattgc ctcgaggatc gcattgatgg cttcatgcgt tgatcgctca cgatcggtgt
     2281 cttcgattcg cagaatgaac ttgccgtaat ggtgacgcgc gaaaaggtag ttaaaaagag
     2341 ccgtccgtgc gcctccgata tgcagatagc ctgtggggct gggcgcgaaa cgagtgcgga
     2401 tctgagtcat cgccgtcaac ctaacaggga gaaaaaatgc ggtcaacggc ggcgcgttta
     2461 aaacagctct gatcgtgata gaatggatta ttgaggagaa tggttgtgga tggcaaagta
     2521 tcagtcgtga aggacgttat ggcacccgca gagggagcgc cgtctaactt tatccgaact
     2581 atcgttgctg acgatctcga gacaggaaag catcacggtc gtgttcacac gcgatttcca
     2641 ccggagccga acggctatct tcacattggg catgctaaat ccatttgttt gaatttcggt
     2701 ctcgcgacag aattcggcgg cctttgcaat cttcgtttcg atgatacgaa ccccagcaaa
     2761 gaagatatcg aatacgtgga gtctattcga gaagatgttc gctggttagg tttcgattgg
     2821 ggtgatcggg agtattacgc ttcagattat tttgatcgcc tgtaccagtt tgcggtgcaa
     2881 atcatcaaag ccggaaaggc ttatgtttgc gatctgaaca gtgatcagat gcgtgagtac
     2941 cgtggcacgc ttaccgaacc gggccggaat agcccttttc gggatcgttc aattgaggag
     3001 aatctaaatc tgtttgagcg catgaaggag ggcgagtttc cggacggcgc gcgcactctt
     3061 cgggccaaga tcgatatgtc ttccccgaat ttaaacctgc gggatccggt gatgtaccgt
     3121 atcctgcatg ctgcgcatca tcggacgacg gatcactggt gtatttatcc cacttatgat
     3181 ttcgcgcacg ggcagtcaga ttccctcgaa ggaatcactc actcgatctg cacattggag
     3241 ttcgaagatc acaggcctct gtacgactgg tttctcgatc agttgggcat tcatcatccg
     3301 cagcaaatcg agtttgcgcg gctcaatcta agccatacag tactgagcaa gagaaagctt
     3361 ctgcaactcg ttcaacaaaa tcatgtctcc ggctgggatg atccgcgaat gccgaccttg
     3421 gcggggttcc ggcgccgcgg ctatacggcc gaagcgatcc gaacattttg cgagcgcatt
     3481 ggcgtggcca agcggaacag cacggtcgat atcgccatgt tagaacactg cctgcgtgaa
     3541 gatctaaaca agcgcgcacc gcgagcgatg gcggtgttga gacccatcaa ggtcgtcatc
     3601 gaaaattatc gtgctgggga agttgaaaat ctagaagcag tcaataaccc agaagatccg
     3661 agtagcggca cgcggcttgt tccgttctcg agagaagtct acatcgagca agatgatttc
     3721 cgcgaagaac cccctaaagg atttttccgg ttatcgcccg ggcgtgaggt tcgcctgcgc
     3781 tatgcataca tcatcaaatg caccgacgtt gtcaaagatc cgcggaccgg cgaaatccgc
     3841 gagctgcatt gtatctacga cccagagact aaaagtggct cttctcgaag taaccgcaaa
     3901 gtaaaagcta ccattcactg ggtctcggcc gcgcacgcca aggaagctga ggtccggctg
     3961 tatgatcacc tgcttagcaa ggaggatccc gacgacgtcc ccgacggcgt cgactggctg
     4021 acaaacgtca atccaaagtc tctggaggtg ctgacggagt gccgggtaga gccgattctc
     4081 gtcaatgcga aatcgggaga gcgctaccag ttcgaaagat tgggctattt ttgcgtggat
     4141 tcggttgatt cgaccgagcg caagccggtc ttcaatcgca cggtaacctt gcgggattcg
     4201 tgggcgaacc tgcagaaggc ggcaaaaaga tccaaatagt tttatgcaat atctgcaagt
     4261 cgccagcgga tctactagca acaacttaaa tttccgttga tcttagaagc gataccccaa
     4321 agaaacagtg acgaaattat tgaaagtttc atatttatct ttgccgggtg cacctgcaaa
     4381 gcccaatggg ctagtggcgg gaaggccctc taactcattg ttggtcactc ttctgtcttt
     4441 gtaaaatacc gccatatacc cgaaatcaac cgaaaagttc tcccacttat agcccacgcc
     4501 agcatttaac gttaacaaat cagcaccagg gatagctgga ttgagcgtat gatttggtat
     4561 cggtgactct tctacggcga gtcctcctcg caactcccag ttcttgttca aggcatagta
     4621 gctgccgaat ctaagtgtgc tagtgttctt ccaatcctca ggaaagttaa acccggaaat
     4681 agggaagcct aagggcaaga aggttgggct tggagagaag gtcgcagtaa aatcttggaa
     4741 ctcgctccat cgggtgtatt catagaccaa ctcggctccc cagttgggtc tcacttgcca
     4801 gaagatgccg gtgtttataa caggcggaag agggatcggc cgaatatctg attttgtagt
     4861 ggctgtagaa aacgagccgc cgagtttgac atctgcatca tcgaaacgca gatccacgcg
     4921 agaccggtag ctaaacccaa ttttaacagt gtctgtaggt ttgatcagcg ctcctatgtt
     4981 ataagcgaaa gcgttggtgg tgtctgttaa ccgcagccga ccttccagcc cgataggcga
     5041 tgtgcaactc gttataggat cgaggcagag tcccctcgaa aacatgccag aggcatggac
     5101 gaagctcgca ccggctccga tggaaaaata ctgggagggg tgccaagccg ctgccggcgt
     5161 tatgtatagg gtcttcaact cggtgcgctc ggcggcaaac cgagtgaaat cactttcgta
     5221 gcttgttgcg agcccaaaag gagaataggc acctatccca aaactaaagt cggtatttgg
     5281 aagatgataa ttgccaaata gcgtcgcgag aaagttgtca tcgtgtttgt taacggctcg
     5341 tctgccacta ttttcgacgc tactaactag accaacgtag gtaccgctca caagcaagtt
     5401 gttgccgtgc acttccccta gtgcggcagg attataaaaa accgctgacg gatcatttac
     5461 gctcgctgtt acggcattag atagacctag cgcccgtcct gtttgatgcg gaatcatgaa
     5521 accaccagcg aacacgccgt gagctaagga aaaaacaaaa attggaccga ccatcatcac
     5581 cgcgattttg acgaacttgt tcctcaccta agactccttc gtcgtgcttg acttgccgtc
     5641 ggcaaacatt atatggatgc aaaacagcgc caaagaatgc cataagatgc aagtagctgc
     5701 aaggataatt gcaagagtcg gcgagcttcg gatctaaaag cgacgcttaa ggattctcaa
     5761 gggcagaagc aatgaatgga tgcagtttta acgtggttgg cagcatatga gcagacaatg
     5821 caaggaaatt cttagcggtg atgacatatc ccttcaggcg aatggcggat ttaccacaga
     5881 accattgata ttgacaaccg ccagtgccaa gaagatagtc gcttatttgg atttgccaaa
     5941 ttcaagcgag tcgcccttgg gaaccgtctt gattgcacct gcatatggag aaacgaagga
     6001 gaataattta ctcatttctg cgtatcttgc tgccaatcgt ttctgtggct tgcgatttga
     6061 ctggagtgat cacgttggag agagtgaagg cgatatttgc acatcgactc tcacaaagat
     6121 gaggcaagtc ttagtttcct tgttggacta tgcacaatta cgcttctcgg agcagcctgt
     6181 tggaatcttt gctagtagct tagcggcccg tgtggccatc aaagtgactg ctcaagatcg
     6241 ccgtccggct ttcctagttt gtctcactcc ggttgtcgat ttacgtgaaa cactaaccat
     6301 ggtctataaa gaggacttgg cgggtaacta ttcagctggg aagagatacg gaactatcga
     6361 tatccttggt ctaactatca acgcggataa tttcttagaa gatgcgatca cgaattcatt
     6421 cgttgatctt atgagcacga aaagagatgc gcgcagtatt gccattccat cgtattttgt
     6481 ggtcggagaa cgtgacacct gggttcgaat agaagatgct aagtcggtag tcgaatgctt
     6541 aagctcagct accaaaacca tagtcgtgtt atctacaatg ttgcaccgct tgcttgaaag
     6601 tccaacaatt gccgtaagcg cgataaaaga gattgttagg aatctgatcg agtgtaccga
     6661 tcggaggaag gctaatccac attcgatcca aataccggat tcaaaccaaa tacgttttcg
     6721 aaagtcgcat gaaagaaatc agctgcggga acgatatgag tattcacaag ctgaggaacg
     6781 aggcttttgg aaggcgtatc tttcgaaatt ccggtatgta ttcaacatac cggattttta
     6841 cgcgttattg gaatcaatcc acaatcgctt aggtggtgtt tgggccgggc aaaaagtgtt
     6901 ggatattggg tgcggggttg gcaattttgg tctatttctg cttaccaagc aattgtatcg
     6961 cgcgcagcag gatctacaat atgcgaagct tgagcctctt cgatattatg gtgtcgactt
     7021 catatgggag gccatctctg cagcctcgcg tcaaatatca gagctgcgaa ctgactttat
     7081 gtcgcggatc ggcttggcta gttcaaatgg cagctttcta aaagagcagt ttgtcttggc
     7141 ggatctggaa gtaggaatcc ctttccccaa tgagttcttc aataacgcgt gctgcaattt
     7201 agttatctcc tatctacaag atcctgctac aatgttaaag gagctttggc gagttctccg
     7261 ccccggagga agtgtcgtca tcagtagctt aaagccgaac gcggatcttt cagaaatcta
     7321 tcggaatttt atttcgttca cagatgatcg gttagaaatc gaagaagggc ggcatctgct
     7381 aaacaatgca gggctgatta aagtaaagga agcggcggga atataccatt tttattccga
     7441 gaaagagtta agagacgttg tgcgtaaggc tggatttcac agggcgaaga cctttcgatc
     7501 ttttggaaat caagcaaatc tcgtggtcgg ttacaaagga tactaatcgc gttatccaac
     7561 cctaaaatag agcttgggag caatgaagat tcgtcacgcc tgaggaaata gtccattggt
     7621 cctcagccgc cctccgaaag gcccgccact taaccgagct taatcttcgt catatgaagt
     7681 tttacgcgat aagcggtctt ttaaaccttg taacatcaag cgtcgtaggt atcttcgtat
     7741 ttactaggga tgttagtgac caacgatatc agacgtacgg agcgttttgc gcaagtctcg
     7801 ccgtctggag tttcggatat ttgttttggc atctgtcgga tacgtcacag gaggccttgc
     7861 tttgggtcaa agtgcttatg gcgggtgcga ttttcattcc tatcacaaac tttcatcaca
     7921 ttgtatctct acttgataag cctccgtggg aggcacggtg gatttttttc ggttactggg
     7981 tttcatttgt gtttctctac ctggatcttt tttctccgtt gtttatcaaa gatgtggcgc
     8041 caaaatctga gttcccgttc tggccggtac ctggtccggc gtttcatgca tatctgaccc
     8101 tttttattta ttgtgttttt cggttcattt atcttctatg gaagcaaatt agggtaaata
     8161 ctgggatcaa gcgaaatcag ttcaagtatt tagtctgtgc tttatcgcta gggtatgtgg
     8221 gcggcggcac aaatttcctt ctgtggtacg acattccgat acgtccttac ggaaacatct
     8281 tagtgtctgt gtatgtcgtt ctcttcagct atgccgtcat caaattttct ctccttgata
     8341 tagatgtcgt tattaaaaag agtctcgtct acgtcctgct actcgttgta ctcattgccc
     8401 cgtgttttgc tctcgtaatc actgcccaag ccctgacatt tggcgagatc aactacgatt
     8461 tttccttatt tacgctttgt gtgttcattg tcgtaggctt cttttttcca aagctcagaa
     8521 tccacgctga gggtgcttta gagaaggtct tgttcaagaa gaaatatgat taccgtgaaa
     8581 cacttctgcg gtcgagcagg gatatgattt cgttgataga cgtagactcg ctatcggagc
     8641 gacttgttcg cacgatcagt tccgccatga gagttgaaaa ggtatccctg tgtattgccg
     8701 atgaatctaa gggtgagttt cgctttacgg catttactgg ctacgcgatc ggcagtttta
     8761 aacatattgc cttaagcaaa gatgatccgt tcattcaaaa ctttcgccga tcatccttaa
     8821 cggttgtaag gg
//
LOCUS       DASUVS010000264         2786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1638525, whole genome shotgun sequence.
ACCESSION   DASUVS010000264 DASUVS010000000
VERSION     DASUVS010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638525"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VF182_14460"
     CDS             complement(<1..786)
                     /locus_tag="VF182_14460"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438336.1"
                     /translation="MKRRPIAVVLLAVFVIAAPKQVLQAAETRISIGVTETMDTFNPY
                     ADSVSLMYSVWCQVLGCLGTYDFDKGQPVGLLVDRWEVKDPKNWIFHLRRDIKFHNGA
                     PLTAEDVVHSINRIRTDPSSKQRQNIAAIASEAVIDKHTVRITTKEPTAPLLDYLFDR
                     IMITNKAVYDKYGPEIADRQYPVGAGPFKFRELIPGQRLVIGKDPNHPMWKQYKQAPD
                     EIVFRIMREPEQRVTALLNNEIQIAQFVPPHLRERVEKSPNHKI"
     gene            complement(803..1786)
                     /locus_tag="VF182_14465"
     CDS             complement(803..1786)
                     /locus_tag="VF182_14465"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438337.1"
                     /translation="MIRIYALLCTVVVCIASVNAVSARDNLPIGYTAITGIKAGLWVA
                     EEAGIFEKYNIQPHLILITSGSKMVQAMLGGDLPLAGAAGNAAVDAALAGADIVMIGA
                     LAKVPAFYIMALPEIKSIEDLRGKSVAVTRFGSSTDFTMRYLLRKHGMNPDRDVTMVQ
                     TGDIFAAAAMMKTKAIVAAPFSSPANLRAQEAGARVLMNMGKAGIYFPHDAWMASRSY
                     INANEDLIRRFMKGYSEGVAKLFSDPDLSRRAIRRFTRATDPKIVDAVYQYAVDYVEK
                     IPYNTREGIQEVLNQAAARNPKAKDAKPEAFYDDRFVKELDSQGFYKQLWK"
     gene            complement(2228..2533)
                     /locus_tag="VF182_14470"
     CDS             complement(2228..2533)
                     /locus_tag="VF182_14470"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX6438338.1"
                     /translation="MLVVSNRIQVSRGFEKEFEERFAGRAGLVDKMPGFIRLEILRPI
                     KSDYYIVLTHWQDEVSFRRWTDSKEFQEAHSNRPRAEIFAGPNVFEMHEVIQHSEKS"
     gene            complement(2561..>2786)
                     /locus_tag="VF182_14475"
     CDS             complement(2561..>2786)
                     /locus_tag="VF182_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438339.1"
                     /translation="HFGAKDPEIIDDAYRSFVPLFPRIPYVNEDGVRDFLADTDHPKA
                     TSADPKTFYDNSFLNELEKSGFVKDLYGQR"
BASE COUNT          666 a          747 c          690 g          683 t
ORIGIN      
        1 gatcttatga ttagggcttt tctcgacccg ctcccgtaaa tgaggcggca cgaattgcgc
       61 gatctggatt tcgttattga gcaaagcggt aacccgctgc tcgggctcgc gcatgatgcg
      121 aaaaacaatt tcgtccggag cctgtttgta ttgtttccac atcggatggt tgggatcttt
      181 gccgatcacc agccgctgcc cgggaattaa ctctcgaaac ttgaacggac cggcacctac
      241 cggatattgg cggtcagcaa tctccgggcc gtatttgtcg taaaccgctt tgttggtgat
      301 catgattcga tcaaagaggt aatcgagtag gggcgcggtc ggctccttag tagtaattct
      361 cacagtgtgc ttatcgatca ccgcctcact ggcaatagcg gcgatgttct ggcgctgctt
      421 gcttgaagga tcggtccgga ttcgattgat cgaatgcacg acatcctcgg ccgtgagcgg
      481 cgcaccgttg tgaaatttga tgtccctccg tagatgaaaa atccagttct tggggtcttt
      541 aacttcccaa cgatcaacca acaagccaac cggctggcct ttgtcgaaat cataggtgcc
      601 cagacaaccc aacacctgac accaaacgct gtacatcagc gaaacactgt cagcgtacgg
      661 attgaaggta tccatggttt cggtaacgcc gatagagatt cgcgtctctg ccgcctgaag
      721 cacttgtttc ggagcggcaa tgacgaagac ggcgagaaga acaacggcta tcggtcgtct
      781 tttcataaac cctccgtcga acttacttcc ataactgttt ataaaatccc tggctatcta
      841 actccttgac gaagcgatcg tcataaaaag cttcgggctt cgcgtctttg gctttagggt
      901 ttctcgccgc cgcttgattc aatacctcct ggataccctc gcgggtgttg tatggaatct
      961 tctcgacata atccacggca tactgataga cagcatcgac gatttttgga tccgtggccc
     1021 gtgtaaatct cctgattgct ctgcgactga gatccggatc gctgaacagt ttggcgactc
     1081 cttcggagta acccttcatg aagcggcgaa taagatcttc gttcgcattg atataggacc
     1141 ggcttgccat ccaggcatcg tgcggaaagt agatcccagc tttacccata ttcatcaaca
     1201 cacgggcgcc cgcttcctgg gctctcaggt tggccgggct ggaaaacggc gcggcaacaa
     1261 tcgccttggt cttcatcatc gcagcggccg cgaagatatc gccggtttgg accatggtca
     1321 catcacggtc aggattcatg ccgtgtttgc gcagaagata gcgcattgtg aaatcggttg
     1381 aagatccgaa ccgggtgacc gcaacggact taccgcgcag gtcctcaatc gacttgatct
     1441 ccggcagcgc cataatatag aaggccggca ccttcgctag agcaccgatc atcacgatat
     1501 ccgcaccggc taaagcagca tcgactgcag cattgccggc ggcgcctgcc aacggtaaat
     1561 cacctcccag cattgcctgc accatcttgg aacccgaggt gatcaaaatc aggtgcggct
     1621 ggatattata cttttcaaaa attccggcct cttccgcgac ccacaacccc gctttgatgc
     1681 cggtgatcgc ggtatagccg atgggcagat tatctctcgc tgacactgcg ttaaccgagg
     1741 caatacaaac gaccacagta caaagcaatg catatattct aatcatctgt ttcttccttg
     1801 gacgctgata ggagggcaaa tcgaaccctg gttagtagat gcacttatac cgatctgacg
     1861 aagcacgctg atgcggacct tatcaactgg cggaaagaag tgtcaagctg actgagcaaa
     1921 aggtaaggtc gacaaccagc ggcaaattcg gatcaaggat gcgcaatttg ttaacggctt
     1981 ttaatgatca cgccgcactc ctcgcagcgg taccgggcaa cgccgctctt ttcctcgacc
     2041 aattcctcga actcgtattg ctttagtccc gaggtgtcga aacattccgg acagaaacca
     2101 tctttcaaat cgtcgcgagg aagggtcaaa tgacagtggc ggcagcgtag tcgactcgca
     2161 ggctcaggca gcaactcgag cgtggtgtgg aagcaggatg acatttttcc ggttgtagac
     2221 gtctgattta gctcttctcc gagtgctgga tgacttcgtg catttcgaag acattgggcc
     2281 cggcaaatat ctcggctcgc ggccggttgc tgtgcgcttc ctgaaactct ttgctgtcgg
     2341 tccaccgtcg aaaagagacc tcatcttgcc aatgggtgag cacgatatag taatcgcttt
     2401 tgatcggccg cagaatctca agccggataa accccggcat tttgtccacc aacccggctc
     2461 tgccagcaaa tcgttcctcg aattcttttt caaaaccgcg agagacctga attctattcg
     2521 atacgacaag catagtttcc tctaatcgca gcaccgattg tcaacgctgg ccatacaaat
     2581 ctttgacgaa cccggacttc tccaattcat tcaagaagct attgtcatag aaagttttag
     2641 gatcggcact tgttgcttta gggtgatcgg tgtctgcaag aaagtcacgc actccgtctt
     2701 cattgacgta tggaattctt gggaacaacg gcacgaaact tctataagca tcgtcgatga
     2761 tctcaggatc cttagcgcca aaatga
//
LOCUS       DASUVS010000265         7597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_108072, whole genome shotgun sequence.
ACCESSION   DASUVS010000265 DASUVS010000000
VERSION     DASUVS010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108072"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VF182_14480"
     CDS             complement(<1..357)
                     /locus_tag="VF182_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438340.1"
                     /translation="MDKRKILWSASLIVALMNVPLASAQDDRRDDGRHDRRDDRRGNW
                     WDGIINGSAASSRDTDRRDLEGTWYLNGDRDKRTQSTSSRDGLEAKNENGDSSRLEIS
                     RNGDVYARDWERGLRGN"
     gene            complement(583..957)
                     /locus_tag="VF182_14485"
     CDS             complement(583..957)
                     /locus_tag="VF182_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438341.1"
                     /translation="MKKGTLIGLLLALAIGVVNIPSSALAQGYGWSSWGNKRSASQHL
                     EGTWYLNGDRSRRAEINESRRGFVARNERGQTTRLEVSRSGDVYARDWQGGLRGDVRH
                     DRIVWENGTIWTREPSYRTARR"
     gene            complement(1133..1402)
                     /locus_tag="VF182_14490"
     CDS             complement(1133..1402)
                     /locus_tag="VF182_14490"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEX6438342.1"
                     /translation="MKRLVASILLSALLLSPLAAWAASSSDDWRNLSPKEKDKVLRNY
                     QRWQHLPPEDKEHLREEWDRWQRLPQDRRERLKRRYEERERRDNR"
     gene            complement(1479..2000)
                     /locus_tag="VF182_14495"
     CDS             complement(1479..2000)
                     /locus_tag="VF182_14495"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6438343.1"
                     /translation="MAAKELETMCKQFEEDLVLYYYGEIGNNEKHKLEQHLADCLGCS
                     RFIEDLHRLLPHIALPEELPQSFWDDYYRETVTKLTHQREQKFHWRNLFAPMRMWMIP
                     AFGTAVIAALAFGVVLSKGNLPALYNQPQERIPREIVTDTEQLEFFRSLDMLEALSSL
                     EGLETSKADSHAI"
     gene            complement(2014..2562)
                     /locus_tag="VF182_14500"
     CDS             complement(2014..2562)
                     /locus_tag="VF182_14500"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6438344.1"
                     /translation="MELVALCQQGEGKFFEILVRRYMEKAFRIALDFTRDTEEAKDLS
                     QEAFLRAFSRIKQFDGRSSFYTWFYRLVVNLCLDHTRRKGKIVWERLESEQDDERQRK
                     ELTDHASGPDQEAIAGETKRRVDKTLDAMPNKQRTAFILRNHEGLPIADIAKVMKTTE
                     GTVRVYLHRAVAALRQCLVEFV"
     gene            complement(2898..3560)
                     /gene="npdG"
                     /locus_tag="VF182_14505"
     CDS             complement(2898..3560)
                     /gene="npdG"
                     /locus_tag="VF182_14505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEX6438345.1"
                     /translation="MENLSVAIVGGTGNLGGALALRLGAPGVRILIGSRDAEKARKAV
                     ETLKPALRAGEMVGMTNQESIRDADYVVIAVPYEGHAQMVQDIKGQVAGKIIIDTVVP
                     LNKGKPFVPPAGSALQEAQQILGNEAPVVGALHNISAVDLADVDSPLGDVLVCGDKAD
                     AKEKVMEIIRRIGARAFDGGPGSNAYVIEGLTGVIISLNRKYKSKHGSIRIVGIGEEP
                     GH"
     gene            complement(3571..4320)
                     /gene="cofE"
                     /locus_tag="VF182_14510"
     CDS             complement(3571..4320)
                     /gene="cofE"
                     /locus_tag="VF182_14510"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX6438346.1"
                     /translation="MNGLAIFGLAGIGEVRAGDSLGKLIVDACSRNGVVLEADDVIVI
                     AQKIVSKSEGRLLPLDGVVVSDYARELGRELDKDPALVELILSESQRIVRRGGRAMIV
                     ETHHGFVCANAGVDCSNVGLRKATLLPKDPDSSAQKIRAEIQELVGASPAIIISDSFG
                     RAWRLGTVDVAVGIAGFKPIKDERGTQDRYGYELRAAVTAIADEIASAAELVMRKKEA
                     MPVVVVRGCEIEKGEGSVKELLRPEAEDLFR"
     gene            complement(4317..5006)
                     /gene="cofC"
                     /locus_tag="VF182_14515"
     CDS             complement(4317..5006)
                     /gene="cofC"
                     /locus_tag="VF182_14515"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX6438347.1"
                     /translation="MKVTALIPVKGFRNAKQRLSALLGSAEREAFAEAMFRDVLRQVL
                     RASGISETVVVTGDDKVAAIAGSLGATVMLERAETGETAAVDFARLSLKDAGHEAVLI
                     LPGDLPLLQSGDIESVLAQVPDTQNAPFALLVPSHDKLGTNALLLAPPDIIKLRFGYD
                     SFSYHISQVSAQGLPIRYFENEHIALDIDEPQDLEKCLANSSDDGEALKTLRQFIAAR
                     RSEPSRRVAEA"
     gene            complement(5036..5956)
                     /gene="cofD"
                     /locus_tag="VF182_14520"
     CDS             complement(5036..5956)
                     /gene="cofD"
                     /locus_tag="VF182_14520"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX6438348.1"
                     /translation="MILALTGGTGGAKLVKGLAAEIDPADLTIICNTADDAIFHGLHV
                     SPDIDTIVYTLAGLNDEEKGWGMAGESFVGLEQLRRLGEEVWFKLGDKDLATHILRTD
                     LLRQGLTLSEATRRIRSALGIQSEILPMSDDRIESRIVTANGEISFQEYFVRERWAPE
                     VKRVFFAGVDQSKPASRVLQAIANAAGIVICPSNPITSIGPILAVPGIRQALREARGK
                     IVAVSPIIARAAISGPAHTLMGTHGLEPSVRGVAKAYADLLDVLIVDREDQSLQSEIE
                     KLGVKPVSADIRMNNLADKRRLARQVLALL"
     gene            complement(5977..7179)
                     /gene="cofG"
                     /locus_tag="VF182_14525"
     CDS             complement(5977..7179)
                     /gene="cofG"
                     /locus_tag="VF182_14525"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HEX6438349.1"
                     /translation="MQQALKECLQGKLLELPEALGLCEVIGDELQELLATAAQLRDMH
                     KGRVVTFSPKVFLPLTNLCRDFCGYCTFRKAPEEAGAKTMTLEEVVRVARQGKLLGCT
                     EVLFSLGDKPETIYPQMKTFLTERGFQRTLDYLFEACLTVLEETAVLPHVNPGVMGQR
                     DLQRLKQVNASMGLMLESTSERLMLNGGAHDRAPDKKPKVRLRTLEQAGKLCIPFTTG
                     ILIGIGESWQERVEALFAIRALHERYGHIQEVIIQNFRAKPTIPMRLHPEPAPEDMVK
                     TIALARLIFGGPMNIQAPPNLTPEGYELYLEAGINDWGGVSPLTPDFINPEAPWPALH
                     RLRERSAAAGFELRARLPIYPEYIYQADRFLSPSILPYVNQLCGKDGLVKNNGPLGKN
                     PETQLPDV"
BASE COUNT         1687 a         2194 c         1933 g         1783 t
ORIGIN      
        1 gtttccgcgc agaccgcgtt cccaatcgcg ggcataaaca tcgccattcc gactaatttc
       61 caaccggctg gaatcgccgt tctcgttttt cgcttcgagg ccgtcgcgag aagaagtact
      121 ttgggtgcgc ttgtcgcgat ccccgtttag gtaccaagtg ccctccaggt cgcggcgatc
      181 ggtgtccctc gaacttgctg cactgccgtt aattatgccg tcccaccagt tgccccgacg
      241 gtcatctcgc cgatcatgtc ttccgtcatc tcttctatcg tcttgggcgc tggcaagtgg
      301 aacgttcatc aatgctacta tcaaactggc agaccatagt atttttcttt tgtccatatg
      361 ctttttcctc cttgcaacct tgttttcgca accgccatgc cacgctctag aaactcgtcc
      421 ctgagcacga tccggttcta aattgtgccg ctcgcgggac ggagtttaaa gatgcgaatc
      481 gccaaaggca cacactggct cacgataaat attcaagacg gatcaaaaga aagggccgct
      541 cgaatcaatc gagacggccc ttttcacgta ggagcaatta agttagcgtc tagcggtgcg
      601 gtagctgggt tcgcgggtcc aaatggtgcc attttcccat acgatgcgat cgtggcgaac
      661 gtcaccacgc agcccaccct gccagtcccg agcatacacg tcgccgctcc ggcttacttc
      721 tagccgcgta gtctggcccc tctcgtttct ggctacgaat ccccgtcgcg attcgtttat
      781 ttcggctctc ctagatcgat cgccgttgag ataccaggtg ccttccaagt gctgcgaagc
      841 actccgttta ttcccccatg aggaccaacc ataaccctgc gctagagcag agctcggaat
      901 gttcacaact ccaatcgcca gagctaacag tagtccaatc aaagtacctt tcttcatgtc
      961 tgaaccctcc taaaggcgtc tgccaactat atggctcgtt cattctgata cttcacttgt
     1021 gtgctctgtc tcttagacgg cttgcgtttg ggttagttta caggcgtgag agcaggcaaa
     1081 agatagaaaa gatacgtatg gcttctccgg agttgcggtc gaactatgaa gtttaccgat
     1141 tatcgcgacg ctcgcgctct tcatagcgcc gcttaagtct ctcccgacga tcctgtggta
     1201 aacgctgcca acggtcccac tcctcgcgca aatgctcttt gtcttcaggc gggagatgct
     1261 gccatcgctg atagttccgt aacactttgt ctttctcttt tgggctcaag ttgcgccaat
     1321 cgtcgctgct gctagctgcc caggcggcca gtggcgatag caacagggcg gagagaagta
     1381 tcgaggcaac aaggcgcttc atcagaattg atcgctctct aataatcagt ttttagaagt
     1441 ttccattaca acaagtaatc gacggatcga gcctcagctc aaatagcatg agagtcggcc
     1501 ttgctggttt ccagcccttc aagagagctt aacgcctcca gcatgtcgag cgacctaaag
     1561 aattccaact gttcggtgtc ggttacgatt tcccttggga ttctttcttg cggttgattg
     1621 tacagcgcag gcagatttcc tttgcttagg accacaccga acgccagagc ggcaatgacc
     1681 gccgtgccaa acgctggaat catccacatg cgcatcggtg caaaaaggtt gcgccagtgg
     1741 aacttttgct cgcgctgatg ggttaatttg gtgacggtct cccgataata atcatcccag
     1801 aaggactgcg gaagttcctc gggcaaggct atgtgcggca gcaatcgatg caaatcttcg
     1861 atgaaccggc tgcagccaag gcaatcggcg agatgctgtt ccagcttgtg cttttcgttg
     1921 ttaccgatct cgccgtaata gtagagcacc aagtcttctt cgaactgttt gcacatcgtt
     1981 tccaactctt tcgcagccat aacctttctc tccttaaaca aactcaacca gacactggcg
     2041 cagcgccgcc accgcgcgat ggagatacac ccgtactgtt ccttcggtag tcttcatgac
     2101 cttggcgata tctgcgatcg gcagcccttc atggtttcgc aagataaaag cggtgcgctg
     2161 tttgtttggc atggcatcca acgtcttgtc gacacgccgt tttgtctccc cggcaatcgc
     2221 ttcctgatcg ggacccgacg catgatccgt cagctccttg cgctgcctct catcatcctg
     2281 ttcgctttcc aatcgttccc agacgatttt cccctttcgg cgcgtatgat ctaaacaaag
     2341 attgacgacc agacgataga accacgtgta aaaacttgag cggccgtcga attgcttgat
     2401 ccgcgagaac gctcgcaaaa aagcctcctg ggagagatct ttggcctcct ccgtgtcccg
     2461 ggtaaaatcc aaggcaatcc ggaaagcctt ctccatataa cgacggacga gtatctcgaa
     2521 gaacttcccc tccccttgct gacataacgc cacaagctcc atatcaggaa gatcttccgg
     2581 cgaacgattt tcaggcgttg cgttaatcga tttaccctgc ctctgttcgt gatctaaggg
     2641 ttcttaaagc cttagacgta gagactggaa aaaggttaac cgcgatagcg gaggggctaa
     2701 aataagcaaa gccagagcac taggtcagta cctgaatcgg ctcccgatgg ggagttaagc
     2761 ggtgagcaac tagaggaata taggttaacc gggtgctgcg tcgctggtgg cttgagtgca
     2821 aagacggatg atccgaaaga attcagaatt gcacaatcat tattacagga tcataggcgg
     2881 tgccaagttt catgatgtta gtgcccgggc tcctcaccga tgccgacaat cctgatactg
     2941 ccatgcttgg atttgtattt ccggttaaga gagatgatga cgccggttag cccctcgatg
     3001 acgtaggcgt tgctccccgg accgccgtcg aacgcgcgcg cgccgatacg ccggattatt
     3061 tccatgactt tctcctttgc gtcagctttg tcgccgcaga ccaacacatc gcccagcggt
     3121 gagtccacat cggccaaatc aacggctgat atgttatgaa gcgcgccgac caccggcgcc
     3181 tcgttgccca gaatctgctg cgcttcctgc aacgccgaac cggctggcgg cacgaacggc
     3241 ttacccttgt ttaacggcac gaccgtatcg atgatgatct ttccggcgac ctgtcctttg
     3301 atgtcctgga ccatttgagc atggccttca taggggacgg cgatcacgac ataatcggcg
     3361 tcgcgaatcg actcctgatt agtcatgccg accatttccc cggcgcgaag agccggcttc
     3421 aaggtttcca ccgctttcct ggctttttcc gcatcgcggg aaccgataag gatccgaacc
     3481 ccgggcgccc ctaaacgaag tgccagcgct cctcctagat ttcccgtccc gccaacaatc
     3541 gccacgctaa gattttccat gatgtttaat ctatcgaaaa agatcctccg cttcgggacg
     3601 caggagttcc ttcaccgatc cttccccctt ttcaatttca cagccgcgga cgacgacgac
     3661 aggcatagcc tctttttttc tcatcactag ttcagccgcg gacgcaattt cgtcggcaat
     3721 cgccgttacc gccgccctga gctcgtatcc ataacgatcc tgggtgccgc gttcatcttt
     3781 gatcggcttg aatcctgcga tccccacggc cacatcgacc gtacccaagc gccatgctcg
     3841 gccgaaactg tcgctgatga taatcgctgg agacgcgccc acgagctctt gaatttcggc
     3901 gcggatcttt tgcgctgagc tgtcgggatc tttcggcaac agcgtcgctt ttctcagacc
     3961 cacattggag cagtcgaccc ccgcgttggc gcagacaaat ccgtgatgag tttcgacgat
     4021 catcgcccgg cccccgcggc gcacaatccg ctggctttca gataggatca actcgacgag
     4081 ggcagggtct ttatcgagct cgcgacccaa ttcgcgggca taatccgaca ccaccacgcc
     4141 gtcgagtgga agcaacctgc cttccgattt cgacacgatt ttttgcgcga taacaatcac
     4201 atcgtctgct tcgagcacaa caccgttgcg tgagcaggca tcgacgatca gctttccaag
     4261 cgaatcaccg gcgcggacct cgccaattcc agcaagcccg aaaatcgcta agccgttcat
     4321 gcttctgcaa cgcgccggct gggttcagag cgccgcgccg cgatgaattg cctcagtgtc
     4381 ttcaacgcct caccatcatc gctcgaattg gcaagacatt tttccaaatc ctggggctca
     4441 tcgatgtcca aggcaatgtg ctcattctcg aaataacgaa tcggtaatcc ttgcgccgag
     4501 acctggctta tatggtaact gaaactgtcg tagccgaagc ggagtttgat aatgtccggt
     4561 ggcgcgagca gcagcgcatt ggtcccaagt ttatcgtgcg agggaaccaa caaggcaaac
     4621 ggcgcgttct gagtgtctgg aacctgcgcg agaaccgatt caatatcgcc gctctgaagc
     4681 agcggcagat cgccgggcaa gataagcacc gcttcatgcc cggcatcttt caggctgagg
     4741 cgggcaaaat cgacggcggc agtttcgccg gtttcggcgc gctccaacat aactgtggct
     4801 cccaatgatc cggcaatcgc cgcaacctta tcgtctccgg tcaccactac agtttcggat
     4861 attccgcttg cgcgtaacac ctggcgcagc acatcgcgaa acatcgcttc ggcaaacgct
     4921 tccctttcgg cgctgccgag aagcgcgctg agacgctgct tggcgttacg aaatcctttg
     4981 accggaataa gtgcggttac tttcatcgat caccggaaat ttcttagcac tattttcaga
     5041 gcaaggccaa gacttggcgg gctaagcgcc gtttgtcggc caggttgttc atccggatat
     5101 ctgccgaaac cggtttgacc ccgagttttt cgatctcgct ctgaagagac tgatcttccc
     5161 ggtcgacgat cagaacatcg agaagatcgg cataggcttt ggccaccccc cttacggaag
     5221 gctcaagacc gtgagttccc atcagcgtat gcgccggacc gctgatcgcc gctcgagcga
     5281 taatcggact gacagcgacg attttgccgc gagcttcgcg caaagcctgg cgaatccccg
     5341 gcacagcaag aatcggcccg atgcttgtga tcggattgct tggacagatg acgatcccgg
     5401 cagcgttggc gatcgcctga agcacgcgcg acgccggctt actctgatcg acgccggcga
     5461 agaacacccg tttgacttcc ggcgcccagc gctcgcgcac gaaatactcc tggaacgata
     5521 tttctccatt cgcagttacg attcggctct cgatccgatc gtcggacatc ggtagaatct
     5581 ccgactgaat gcccagtgcg ctgcgaattc gccgtgtcgc ttcagagagg gtcaagcctt
     5641 gccgtagaag gtccgtgcgc agaatatgcg ttgccaaatc tttatcgcca agcttgaacc
     5701 atacttcctc gcccaaacgc cggagctgtt ccaaaccgac gaagctttcg ccagccatgc
     5761 cccaaccctt ttcttcgtcg ttgagaccgg cgagcgtgta gacaatcgta tcgatatcgg
     5821 gcgaaacatg aagcccatga aagatcgcat catccgcagt attgcagatg atcgtcagat
     5881 cggcgggatc gatctccgcc gcgagacctt taacgagttt ggcgccgccg gtaccgccgg
     5941 tcaaagctaa gatcatggct aaacgggtcg cggcggctag acgtcgggca gttgagtctc
     6001 cgggtttttg cccaatggac cattgttctt taccagtccg tccttgccgc atagttgatt
     6061 gacatagggc agaatacttg gggataggaa tctatcggct tgataaatgt attcagggta
     6121 aataggtaat cgcgctcgca gctcgaatcc cgccgctgcg ctcctttccc gtagtctatg
     6181 gagagccggc catggcgctt caggattgat aaagtccggg gtcagcggcg ataccccgcc
     6241 ccaatcattg atgccggctt ccaagtaaag ctcataaccc tccggcgtca ggttgggtgg
     6301 ggcctggatg ttcatggggc cgccgaatat caaccgggcc aaagcgatag ttttaaccat
     6361 gtcttccggg gcaggctccg gatgaagacg catcgggatg gtcggtttgg cgcgaaagtt
     6421 ttggataatt acttcttgaa tatggccgta gcgctcatgc agcgcgcgaa tagcaaaaag
     6481 agcctcgact ctttcctgcc agctctcgcc gatgccgatc agaatgccgg tggtaaaagg
     6541 aatacacagc ttgccagcct gctcgagagt gcgtaaacga accttgggct ttttgtccgg
     6601 cgcacgatca tgagcgccgc cgttgagcat caagcgctcg ctggtgctct cgagcatcaa
     6661 ccccatactg gcgttcacct gcttcaagcg ctgcaaatcc ctctgaccca tgaccccagg
     6721 attaacgtgt ggcaacaccg cagtttcttc aagcaccgtt aggcaggctt cgaatagata
     6781 atccaaggtg cgttggaagc cgcgttcggt taggaaagtt ttcatctgcg gataaatcgt
     6841 ctcaggcttg tcgcccaaac tgaaaagcac ttcagtgcag ccaagcagtt ttccctgccg
     6901 cgccacacga accacttcct ccagggtcat ggttttggcg ccggcttcct ccggcgcttt
     6961 gcgaaaggtg cagtaaccgc agaagtcgcg gcacaaattc gtcaacggca aaaatacttt
     7021 cggcgaaaaa gtgaccactc tgcccttgtg catgtcgcga agctgcgctg cggtggcaag
     7081 caattcctgt aattcatcgc cgatgacttc gcaaagccca agcgcctcgg gtaactcgag
     7141 caattttcct tgaagacatt ctttcaatgc ttgctgcatc agcctgtgca tatcggatcg
     7201 ctataattgg gtcaactgca cttcaatcgt aaaatcatgg ctgttcgcaa aatgatcaac
     7261 aaggtaacgt ccacacgatt cgagtcgcgt agcgactaag gacagtgtca acacgaccaa
     7321 atacgggtct catgactcgc ttgagcgaag ctattctgaa tgatacgagt ctcggttgat
     7381 aacgatgtga atttcaccaa caaagcggcg gcacagtttg gcggcctgtt gccgaatcat
     7441 tagcgtgaag atagaacatc agaaaaacgg tgagggggga acggatcatg gataactttg
     7501 acgatgtgct tgtgatagat gccgatggcc acgtctacga aggcaacgtc gatctcgcca
     7561 gccggatgcc cgagaagtgg cgctcgcaag ctcccgt
//
LOCUS       DASUVS010000266        17153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_764549, whole genome shotgun sequence.
ACCESSION   DASUVS010000266 DASUVS010000000
VERSION     DASUVS010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 17153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17153
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764549"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..960)
                     /locus_tag="VF182_14530"
     CDS             complement(76..960)
                     /locus_tag="VF182_14530"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX6438350.1"
                     /translation="MDLIRSWMFVPGHRQRMIDKALGLNADAIMLDIEDGVAPNEKDS
                     ARKNIAESLGRDKAPNTPSRYVRINAIGHARMDADLEAVVRPGLEGLVCPKVDTPEEI
                     RKVDQILMEREPKLGMQKGAVKLLIAIESPKGLLNAPAIAASSPRISGLIFGAEDFGR
                     EIGLPTSREGEARDLIYARSAIVVAAAAAHVQAVDGVWVDLNDSEGLLNFARQARQLG
                     FSGMSCIHPSQVDAINTTFSPTADEITYAREVLKAFEEANARGDGSIAMGGQLIDRPI
                     VERARRTIDMAKALEIAT"
     gene            1110..2300
                     /locus_tag="VF182_14535"
     CDS             1110..2300
                     /locus_tag="VF182_14535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6438351.1"
                     /translation="MKASTVKLLVPLVAITIQSNSASTIIPPYLDYLHIPVGVIGSLI
                     SLGPVLALTSRLPVGMAYNHHRARLLITLAVLAMGATNFAYSFASDTIAFALIHSLNG
                     FAYGAVTTLYMAFYVDSLAADENRNHAMGYYVGSLALGYSTGNFFGGLFADHFGYAIT
                     FQGAAFLSLVPCALLWLLRAPAGQGGGNAKEKPKVKLMSKEFWRALLEPGLATVVVTA
                     LFLNLLHQMGGVFISLYGLAVGMTLTQIGIIRAAYAGVNAVTRPISGHVVDKVGHRRL
                     SYAGIPLQSALLMLIPLFSGFGPILIVYIMSGLMRAIVIVANAVGLVQDVDESKVQRG
                     MASAVYNSAGDVGNIIGPSVGGIIAHATSIGAVFVYGSLGSTILFLLVIFAVRRSGRM
                     NYES"
     gene            complement(2366..3526)
                     /locus_tag="VF182_14540"
     CDS             complement(2366..3526)
                     /locus_tag="VF182_14540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6438352.1"
                     /translation="MDFNLTPEQQQIREEIKRVCRDFPDSYWREIDSKKEYPEAFVRK
                     LSELGWLAALIPEEFGGTGLGITEASIILEEINHSGGVATACHAQMYTMGTLLRHGND
                     EQKKRYLPKIATGELRLQAFGVTEPDAGSETTKLQTTAVRKGGKYLINGQKIFISRVL
                     QSDLMLLLARTTPYDELEDKTRGLSVFIVDLRENKDKLEVKPLDLMINHHTNQLFFDN
                     VEVPVENLVGQEGMGFRYIIDGWNAERILVAAEAVGDGRWFVERAAKYASERIVFGRA
                     IGSNQGIQFPIAQAYAQIEAADLVRYQAATKFDRNEKCGAEANMAKLLASEAAWAAAN
                     ACLTTHGGYGFAAEYDVERKFRETRLLTVAPVSNNLVLAYLGQHVLGMPKSY"
     gene            3751..4263
                     /locus_tag="VF182_14545"
     CDS             3751..4263
                     /locus_tag="VF182_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020266227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX6438353.1"
                     /translation="MAVKPGWEGRYFEDFEIGDVYRCRLGRTVTEADNIWFTLLTNNT
                     NQIHFNNEYGKRTEFGKCLINSALTLAIVAGMGVADVSENGFALGWDEIKLPNPLFAG
                     DTLYSESEVLEKRESKSKPQWGILKVRTRGVQQEGKVVIDYARSVMVWKKAHAPKRDL
                     FPTIREDNKS"
     gene            4450..5649
                     /locus_tag="VF182_14550"
     CDS             4450..5649
                     /locus_tag="VF182_14550"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX6438354.1"
                     /translation="MASSVKPNRALDGVKILAFEQVLSGPFATCLLADMGAEVIKVER
                     PGVGDVIRSWDSVVKGLSSGYVWLNRNKRSLTVDVKKEKGKEILRQLASKSDIFFENY
                     APGVAARLGLGYEELSQSNPRLIYCSLSGYGQDGPYRDVKAYDLLIQGEGGIIATTGY
                     PEKPAKAGIAIADIASGMYAVIGIVLALYQREKTGEGQFIDVSMLDSIVSWLGYFPHH
                     YWHAGEEPGRAGMRHHYVTPYGPYMAADGEYVNLAVASPADWEQFCQVVIERPDLLKD
                     SRFRTVQDRRKNRGVLEEIIEAIFLERDHKHWLAQLKKAEMPHGIVRGIAQVLAHPQV
                     AARKLIREADSPVGKVPVIANALKMTDSPARYDRIPDLGEDNDAILQELGYEAAAIAD
                     LRRDKVI"
     gene            5844..6395
                     /locus_tag="VF182_14555"
     CDS             5844..6395
                     /locus_tag="VF182_14555"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438355.1"
                     /translation="MTKCVIGVMGASESDALTDAEKARLKQLAEKLGCAIAKHDCILI
                     SGATTGLPDLISRSAREHGGFAVGISPAENRREHIETYKMPPDAADVIIYTGFGLKGR
                     NVVNVRSSDIVIIFGGATGTLNEFTIAYDEGKIVGVLEGTGGCADHIKEVIDFCKKPS
                     RATVLFDTDPEKLIEECLGCLEN"
     gene            complement(6685..6822)
                     /locus_tag="VF182_14560"
     CDS             complement(6685..6822)
                     /locus_tag="VF182_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438356.1"
                     /translation="MTASHALKKVALTGVEALVLCHTKQVEHGPAIAEIAVESEPTVV
                     G"
     gene            complement(6950..7834)
                     /locus_tag="VF182_14565"
     CDS             complement(6950..7834)
                     /locus_tag="VF182_14565"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6438357.1"
                     /translation="MMNDPKFIDVDRIRTRYFDKGSGEVLLLVHGSHMGTPDACESAI
                     DWEFNFDVLAQSFRVIAMDKLGQGYTGNPKSDADYTMAATVKHAAGLLQALGLKNVHV
                     IGHSRGGYVVTRLTLDHPDLVRSCVIVDSGTLAPGPSKTEFIMANAPKPRLSRESQRW
                     CIEHYSFRPNHITQNWLDTCEDIAKSEKYQATVRKMEDQGLKRNQFFTHLALHKEETL
                     NWIMHGKLTQPTMLVWGYNDPTAELKRGHALFEIIADSNPRSEMHIINEAGHFCYREQ
                     PETFNGIVKAFVEKCAKL"
     gene            complement(8015..9037)
                     /locus_tag="VF182_14570"
     CDS             complement(8015..9037)
                     /locus_tag="VF182_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6438358.1"
                     /translation="MDNVEPKGAAHWIDHFVVGTNDMVAWTDWAVNGIGLIRRPIRGL
                     TTEARKKNIKIICFLWWEGGSCRIGAFLQPEIYPAAKELGEELPRCGFYIRPEDIDGH
                     LRRLDRYEISHTDPVKTAAEGEEGTAIYFADPDGNQYEFWAPMHMPEGAMEIATDEKV
                     GRISHAVYGCRDLARTAAFFEKYCGIQAEQNSKTAEGSLVLRLRAGARLIYKLVDKVD
                     ERVAGHGPWWDMHTALTVREEEFFPNYRRMWDGLPEEKTDKANADLSIEQQEALPART
                     GLHRSPVGRKWKEICDRGDEFYDWDAHAFHFIGGIPLKTNGSMALYQAKEQEEYLREL
                     AESLKG"
     gene            complement(9394..9732)
                     /locus_tag="VF182_14575"
     CDS             complement(9394..9732)
                     /locus_tag="VF182_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX6438359.1"
                     /translation="MSNLTLHPMKEIRIIIQGEHLKFATDLLDNKATGYTIIHNISGK
                     GHHGFHTAHPMFNEIDSIVMLMSVVAPEKVEPILAGLKPLFDRYTGVMFVSDVAVSRV
                     EYFSAGAGSK"
     gene            complement(9745..12900)
                     /locus_tag="VF182_14580"
     CDS             complement(9745..12900)
                     /locus_tag="VF182_14580"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HEX6438360.1"
                     /translation="MAAAQYSETQRMELRALILLASEVIADYWPMRTFVHHNPLHGLE
                     YLHFEEAVSRAQRLIEAKRYLPVEIFRDYFCSGRILPDHLDLALRGRAENRQVQLGEH
                     NLTLGAVLRACMLQDLSPPALNTFDALLKRHPDRSTIKSLASHLVSAAKPPQSGRDTA
                     EQRDLGRTLTLATWCDRTLGTAIVEQINRELVKWCEAFLDEGHATWPMPRREQGFYGA
                     WKFLAQKEWSPGAIENSREKLARLPAQPEDALLESLATLGIPADARQDYFSLHLVALS
                     GWAGFIKWRGDQSEYEWQLAYPIDLAQYLAVRLWYERELVQKACRESLGIDGNLDAIS
                     TALQRRKDLSRKHSGQPPAESLAALVAAWRLYALAQALGHDPVILTQSQPGNLLTLLD
                     WIDGFPEADHGPVWLKAFEAAYQEQLLGKLAVNLDQPGEAASGPSSSHRAAAESESQR
                     ETKVRHQAQAVFCIDVRSEPIRRHLEAIGDYETFGFAGFFAVFIRYQGLGSDHVTDQF
                     PVIMKSKNLVREIPRTYQGQYLFRRRHREKLLETGHTLLYDLKENVVTPYVMVESLGW
                     FYSLPFIGKTLSPTWYRKVMSWLRRKLVPPVATTLTVDKLSREEVEEMLAAEQRAVTR
                     RALQENFGERHLNLSLERLEFLRRRALYSESAGEPSPQARSTALSREEEAAFVEDLRR
                     NYGINPGASFARMERITRTGFTLSEQVFTVETALRIMGLTRNFARLVLFCGHGSTSEN
                     NPFEAALDCGACGGNPGKPNARVLAIMANKPLVREQLAKNGIVIPQDTYFVAGQHNTT
                     TDDVDLIDLEDLPPTHRKDLLQLVRDLESAGTLNSQERCARFPETKTSLPASKARRTV
                     HRRSADWSQVRPEWGLSGNAAFIIGRRALTRGINLDGRAFLHSYDYREDFDGQLLEVV
                     MTGPQIVGQWINMEHYFSAVDPENYGSGSKIYHNVVGRFGIMSGPQSDLRTGLASQTV
                     MNGIRPYHEPMRLVTLVEAPRERIDKIIRNQRHLKRLYDNEWLYLIAIEPEEQLAYRY
                     VSKEGWQAVKPSSSSPRQRFV"
     gene            complement(12912..15482)
                     /locus_tag="VF182_14585"
     CDS             complement(12912..15482)
                     /locus_tag="VF182_14585"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX6438361.1"
                     /translation="MAYYPSLILFFPLAAGLIIGLLGRAMGPKAVRLGVAAEVISFTL
                     SLLVLYEVTSRGPQNIGLFSWSDGILQFGLHIDRLSSVMLVLIATISILIHLFSIRYM
                     QQERGYTRFHSLLAFTTFALFGMVSSANLLILFIFWQLLSWLVPLLSFNHSHPLTVRG
                     AFRTFIMQRIGDVAFLAAILLAYGSYGTLEFQELFSRAAEVQTSLFLGPGDRFEMRAD
                     TAVTLLLFIGAMTKSAQFPLHMWLPDSLYAPTPVTALLHAGIINAGGFLLTRLAPLYD
                     LNPGTLHLVFAVGLLTTILGSSMMLVQNDIKKTLGYSTIGQMGFMIMECGLGAYGLAI
                     FHLMAHGLFKATIFLNCGYVIHAARQEPRLPPKAKAIERRQFSTLTWVTGFITTLILP
                     LMILLAAHGILRIPVGDSQGTTIFLFFGWATSSQAILTLYRLRAVASWKVAASMLFTL
                     FLVVVTYLLAAESFTHFLFPARGEVAVHFQAGALPVLVFDILVSAFALVIILGWVLIY
                     AESHGRSIGMPEWVNSLRIRIYLLLMNRLYLDAVAIRFNRVLRREIDRLNGSRIFSYL
                     AALIAIGSAVTLGGRLPVVPAEIGLSILVAILLPLFPLHGIYVAALTRWQGYQAIALA
                     VLLPAAGLFAIAQLMPDLPAETLRAVRVLALFGAVYGSFKALAQTRVPQVVAYAGVAF
                     FSALWWSFGVAGTFAFQPIIYCATTVLISAGLLLAWQTVRARYGNLSLEAMHGLATPM
                     PRFATVLSLLIMAAVGLLPLGLFSAYLAMLVQASIALSWGLMVVLFSWFLASWYLFRM
                     MQQLLFGPHRSDTRYTDLEVSEVAGFALLLLILIALGVMPHDWLETVVLAHVGRMPWR
                     "
     gene            complement(15749..16786)
                     /locus_tag="VF182_14590"
     CDS             complement(15749..16786)
                     /locus_tag="VF182_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX6438362.1"
                     /translation="MRATIMYSAGDVRIEDVPDADIVEPTDVVVAVSRACICGSDLWP
                     YRGMEEIPGGGRRMGHEAIGVVQAIGAEVRTMKAGDLVVMPFAYSDGVCTFCHEGLQT
                     SCVQGGFFGIAGMNGAQAEALRVPLADGTLFVLPVGQDDALMPSLLTLSDVMGTGHHA
                     AVAAKVGPGKVAAVVGDGAVGLCGVIAAKRLGTEQIIILGRHADRIALAREFGATDVV
                     SERSEEAIARVRELTRGFGVHSVLECVGVEESMRTAISIARPGGAVGRVGVPQGETLP
                     ASLPAFYNNVTVSGGPAPIRAYIEELLPDVIEGRIEPGRVFDRVVGLDEVPDGYRAMD
                     QRKSIKVMVEP"
     gene            complement(16792..>17153)
                     /locus_tag="VF182_14595"
     CDS             complement(16792..>17153)
                     /locus_tag="VF182_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6438363.1"
                     /translation="GCVRIDPLFEAPEPARARGAIVTFEPGARSAWHTHPLGQTLIVT
                     AGCGWVQSEGDPKVEIRPGDVVSCPPKEKHWHGATTTTAMTHIAIQEALDGKVVEWME
                     KVSDEQYQTAQPPGNER"
BASE COUNT         3820 a         4773 c         4782 g         3778 t
ORIGIN      
        1 acacgtacca cgaacgcgaa tttggaggtg agaccaaggg aacacaccga agcatcgaac
       61 gcattaacag cgcgcttacg tggcaatttc tagcgccttc gccatatcga ttgtccggcg
      121 ggcgcgttca acgatggggc gatcaattaa ctgcccgccc atggcaatgg aaccgtcgcc
      181 gcgggcattg gcctcttcga aggcttttaa tacctcccgc gcataagtta tttcatccgc
      241 ggttggactg aatgtcgtgt tgatggcatc aacctgtgat gggtgaatgc aggacatgcc
      301 ggagaagccc aattggcgtg cttgcctggc aaagttcagg agaccctcgc tgtcgttcaa
      361 gtcgacccag acgccatcca ccgcctgcac atgcgcggcc gcggcagcaa cgacgatggc
      421 ggatcgcgcg tagattaggt cgcgcgcttc gccctcgcgc gaagtcggca accctatttc
      481 acggccaaaa tcctcggcgc cgaaaatcag cccgctgatg cgcggcgagc tcgcggcaat
      541 tgccggcgca ttcaataaac ctttggggct ctcgatggcg atcagcaatt tgacggcgcc
      601 cttttgcatt cccagctttg gctcacgttc catcaggatt tgatcgacct tgcggatttc
      661 ctcaggcgta tccactttgg ggcaaaccag tccttcgagg cccgggcgca cgacagcttc
      721 cagatcggcg tccatgcgcg catgtccaat ggcattgatc cgcacatagc gcgacggtgt
      781 gttcggtgct ttgtcgcggc ccagtgattc ggcgatgttt ttgcgcgcgc tatctttctc
      841 gttgggcgcc acgccgtctt cgatgtcgag catgatggca tcggcattta ggccgagcgc
      901 tttatcgatc atcctttgcc ggtgccctgg cacaaacatc catgagcgta tgagatccat
      961 tctccgttct cctatcgaag ttcaaatacc tgaaggactt ggaaatgtct atcttcacag
     1021 tattcggcgg catgatttgc aaccgcggtg cttttagaga ataaaagaga gatgatttat
     1081 caatcctcga aattccaaat caatcgacga tgaaggcgag cacggttaag cttctggtgc
     1141 cgctggttgc gatcacaatc caaagcaatt ccgcatcgac tatcattccc ccctatcttg
     1201 attatctcca cattcctgtt ggcgtgatcg gcagtttgat ttcacttggg ccggtgctcg
     1261 ccctgacatc gcgtctgccg gtcggcatgg cctacaacca tcatcgcgct cgacttctga
     1321 tcaccctggc agttctcgcc atgggagcga cgaacttcgc ttatagcttt gccagcgata
     1381 cgattgcgtt cgcactgatt cattccctca atggttttgc atacggcgca gtgacgacac
     1441 tctatatggc gttctatgtc gattccttgg ctgctgatga gaaccgtaat cacgctatgg
     1501 gatactatgt gggtagttta gcgcttggct attcgacggg aaattttttc ggcggtctat
     1561 ttgccgatca tttcggttac gcgattacgt ttcagggcgc cgcgttcttg tcgttggtgc
     1621 cgtgcgcgct gttgtggctg ttgcgagctc ccgccggcca ggggggaggt aatgccaagg
     1681 agaagccgaa agtaaagttg atgtcgaaag agttttggcg agcgctgctc gaaccgggac
     1741 tggccaccgt ggtggtgacg gcattgtttc tcaatttgct gcaccagatg ggcggcgtat
     1801 ttatctcgct gtacggattg gcggtgggca tgacccttac gcagatcggc atcatccgcg
     1861 cggcttatgc cggagtcaat gccgtcacgc gcccgatcag cggtcatgtc gtcgataaag
     1921 taggccatcg ccgtttatca tacgcgggca ttcctctcca atccgcgctt ctcatgttga
     1981 tccctttatt cagcggtttc gggccgatct tgatcgtcta cattatgtcg ggcttgatgc
     2041 gcgccatcgt catcgtcgcc aacgcggtgg gtctggtgca agacgtcgat gagagcaaag
     2101 tgcagcgcgg tatggcttcg gcggtttata actcggccgg ggacgtcggt aatatcattg
     2161 gtccgtccgt cggcggaatc atcgcccacg ccaccagcat cggggcggtg tttgtttatg
     2221 gatcgctggg ctcgacgatt ctattcctac tggtaatttt tgccgtgcgg cgaagtggaa
     2281 gaatgaatta tgaaagctga tagctgaaaa cgaacccggc tcgatcctga tttccgcgtt
     2341 ccgttttctg ctctcatctt tgtcttcaat aggacttcgg catgcccagg acgtgctgtc
     2401 caagataagc cagcaccaaa ttgttgctca ccggcgcgac ggtcagtagc cgtgtctctc
     2461 tgaatttcct ttcgacatcg tactccgcgg caaaaccgta gccgccgtgg gtggtcaggc
     2521 aggcgttggc agccgcccag gcggcttcgg aggcaagaag cttggccatg ttggcttctg
     2581 cgccgcattt ctcgttgcga tcgaatttcg tcgcagcctg atagcgcacc agatccgccg
     2641 cttcgatttg cgcataggcc tgcgcgatgg ggaattggat gccctgattc gaaccgatgg
     2701 cgcgaccgaa gacaatccgc tccgaggcgt acttggccgc gcgttcgacg aaccaccgtc
     2761 catcgccaac ggcttcggct gccactaaga tcctttcggc attccagccg tcgatgatgt
     2821 agcgaaaacc catcccttcc tggccaacga gattctccac tggtacttca acattgtcga
     2881 agaatagttg attggtatgg tgattaatca tcaagtccag cggcttaact tcaagcttat
     2941 ctttattttc tctaaggtcg acgataaaaa ccgagaggcc acgggttttg tcttccagct
     3001 cgtcgtaagg tgtggtgcgc gccagcagca gcatcagatc ggattggagc acccgggaga
     3061 tgaagatctt ctggccattg ataagatact taccgccttt acgaacggca gtggtctgga
     3121 gcttggtggt ctcggatccg gcgtcgggtt cggtgacacc gaacgcttgc aggcgcaatt
     3181 cgccagtcgc aatcttcggc agatatcttt tcttctgctc gtcattgcca tgcctgagaa
     3241 gcgtgcccat cgtgtacatc tgcgcatgac atgcagtcgc cacgccgccg gagtgattga
     3301 tttcttccag gatgatgctc gcttcggtga tgccgagacc ggtgccgcca aattcttccg
     3361 ggatcaacgc ggcgagccag cccagctcgg agagtttgcg cacgaatgct tcgggatatt
     3421 ctttcttgct gtcaatctcg cgccagtaag aatcgggaaa atctctgcac actcttttga
     3481 tttcttctcg gatctgttgc tgctccggcg tcaggttgaa atccatgcct gcctcctata
     3541 attaggcccg tagcaggctg ctgaaaaagc ctcatgtgct tcgttgcgct cgatcgcctc
     3601 gcttcgacgt actgccgatg tacgcctcag ctcgttgatt cttcgcgcgc ctcgcctctg
     3661 agatcttttt gagcagcctg caagagaatc aatcagcact cgttagacta tagcgaaata
     3721 gtgttcgttg ccaatcgagg gaggaaggtc atggcggtca aaccgggatg ggaaggtcgg
     3781 tactttgaag attttgagat cggcgatgtc tatcgttgtc gattgggacg aacggtcacc
     3841 gaggccgaca acatctggtt tactctgctc accaataaca ccaatcagat tcactttaac
     3901 aatgaatacg gcaagcgtac cgaattcggc aaatgtctga tcaacagcgc tttgaccctg
     3961 gcaatcgttg ccggcatggg cgttgccgat gtcagcgaaa atggtttcgc cctgggctgg
     4021 gatgaaatca aactgcccaa cccgctgttt gccggagata cgctctattc ggaatccgaa
     4081 gtgctggaga aacgcgagtc gaaatcgaag ccgcagtggg gcatcctcaa agtgcgcacc
     4141 cgcggtgtcc aacaagaagg caaagtcgtt attgattacg cgcgcagcgt catggtttgg
     4201 aaaaaggcgc atgcgccgaa aagagatctc tttcctacca ttagggaaga taataaatcg
     4261 tagagcttac gggttgagcc tcgattctaa gaaactatct tgttccggct gttccaatcg
     4321 tttcaatggt tccatagtta aagatccaac ctaaactcaa ccggggtgcc tcaggtttga
     4381 atttgaccga ctcgaacgat tgaaattact ggaacagttg gaacctgttc acatagaaag
     4441 aggtaactcg tggccagcag cgtaaaacca aaccgtgctc tagacggagt taaaattctt
     4501 gctttcgagc aggtcctctc cgggccattt gccacctgtc tgcttgccga catgggcgcg
     4561 gaggtcatca aagtcgaacg gcccggagta ggggatgtca ttcgttcctg ggattccgtt
     4621 gtcaaaggcc tatcgtcagg atacgtttgg cttaaccgca acaagcgcag cctgaccgtc
     4681 gatgtcaaaa aagaaaaagg caaagagatc cttagacagt tagctagcaa gtccgatatt
     4741 ttttttgaaa actatgctcc tggcgtggcc gcgcggctgg gtctcggtta tgaagaactc
     4801 agccagagca atccgcgatt gatttattgt tccctatcag gctacggcca ggacggaccc
     4861 taccgcgatg tcaaagcata cgatctgctg attcagggcg agggcggaat cattgcgacc
     4921 accggctatc ctgaaaagcc cgccaaggca ggcattgcga tcgccgatat cgcctccggg
     4981 atgtacgcgg ttatcggcat tgtcctggcg ctctaccagc gcgagaaaac cggtgaggga
     5041 cagtttatcg acgtgtcgat gctcgactct atcgtctcgt ggctcggtta tttcccacac
     5101 cactactggc atgcgggaga ggagccaggc cgcgccggca tgcgccacca ttatgtaaca
     5161 ccgtacgggc cgtatatggc ggccgatggc gaatacgtaa atctcgcagt cgccagcccg
     5221 gccgattggg aacagttttg ccaagtggtg atcgaaaggc cggacctgct aaaagattcg
     5281 cgctttagaa ccgtccagga tcgacgaaag aaccgcggcg tgctggaaga aatcattgaa
     5341 gcgattttcc tcgagcgtga tcacaagcat tggctggcgc agctcaagaa agccgaaatg
     5401 ccgcatggca tcgtccgcgg cattgctcag gtgctcgccc acccgcaagt ggcggcgcga
     5461 aaactgatcc gtgaagcgga ctcgccggtg ggcaaagttc cggtgattgc caacgccctc
     5521 aagatgaccg acagccctgc acgttatgac cgcatccccg atttgggcga ggacaatgac
     5581 gcaatcctgc aagagcttgg atacgaagcg gcggcgattg ccgatctgcg gcgcgacaaa
     5641 gtgatttaag ctcttgcgtt gcgataaaaa gtcacaccga acagccaacc gattgcacct
     5701 cgaaggacac gaagtaaaat agcctcggat gaaaggagct caagcgcttt atttcagtcg
     5761 gattcgtctc tgcgagatga tttgctgatt cacctccgtg tccttcgtgt gcttcgtggt
     5821 gagaactctc caaaggatga ttcatgacta aatgtgtcat cggggtgatg ggcgcttcag
     5881 aaagcgacgc gctcacggat gccgagaaag cccgcctgaa gcaactcgca gaaaaactcg
     5941 gctgcgccat cgccaagcac gattgtattt tgatctccgg agctacaacg ggtctgccgg
     6001 atctgatttc gagatccgcg cgtgaacacg gcgggtttgc tgtgggcatt tctcctgccg
     6061 aaaatcgccg ggagcacatc gagacctaca agatgccgcc cgatgccgcg gatgtgatca
     6121 tctataccgg ctttggcctc aagggccgca atgtcgtcaa cgtgcgctcg tcggacatcg
     6181 tcatcatctt cggtggcgca accggcactc tgaatgaatt caccatcgcc tatgacgagg
     6241 gaaagattgt cggggtcctc gaaggcaccg gcggctgcgc cgatcacatc aaagaagtca
     6301 tcgacttctg caagaaacca agccgggcta cggtgttgtt tgataccgat ccggagaaac
     6361 tgatcgagga gtgcttaggt tgtctagaaa attaaccgac cggcgatgag cggagccaaa
     6421 ttttgaattc tgtcggcaga attgaaactt gtccatgcgg tagcggtctc aaattcaaga
     6481 agtgctgtct gagccagact gccgcagcga cgaaatctta cagttccgta gatcgcgacg
     6541 aagcgttgtt gaagctcatg cgtttcgcga cccgctctga attcaaggaa gcataccgcg
     6601 ttgcgttcca acgttctggg gcgactggct ttcggaagaa ccggatgagg agttaaggga
     6661 tgtcatgacc tccgagacgg ttaactagcc taccacagtt ggttcgcttt cgactgcgat
     6721 ctcggcgatg gccggaccgt gttcgacctg ttttgtgtga cagaggacaa gagcctccac
     6781 gccggtgagc gcaactttct tcaaggcatg cgaggcagtc acgcaggtaa acccgcctat
     6841 gattcagttt ggctgtggaa ggaactgaac atcacaagag agtgagaaga ggcggtgaaa
     6901 agcgcccgcg cgccaattac agattcgaag atcccgcgga actgccaaat cacaatttcg
     6961 cgcacttttc gacgaacgct ttgacgattc cgttgaatgt ctccggttgc tcgcggtagc
     7021 agaagtggcc ggcctcattg atgatgtgca tttccgatct gggattgctg tcggcaataa
     7081 tctcaaaaag cgcatggccg cgtttgagct ccgccgtcgg atcgttgtaa ccccacacta
     7141 acatcgtcgg ctgggtcagc ttgccgtgca taatccaatt aagcgtttct tccttgtgca
     7201 gagccaggtg agtgaagaac tggtttcttt taagtccctg gtcctccatt tttctgacgg
     7261 ttgcttggta tttttctgac ttggcgatgt cttcacaagt atccagccag ttctgcgtga
     7321 tgtgattcgg cctgaacgag taatgctcga tgcaccagcg ctggctttcg cggctgaggc
     7381 ggggcttggg cgcattggcc ataatgaatt ccgtcttcga gggcccgggc gcaagcgtgc
     7441 cgctatcgac aatgacgcaa gagcgaacca ggtccggatg atccagtgtc agccgcgtga
     7501 cgacgtaacc gccgcgcgag tggccgatga cgtgaacatt cttaagcccg agcgcctgta
     7561 agagacccgc cgcatgcttt accgtcgccg ccatggtgta gtcggcgtcg gatttcggat
     7621 tgcccgtata gccttggccg agcttgtcca tggcaatgac gcggaacgac tgggcgagaa
     7681 cgtcgaaatt gaattcccaa tcgatggcgc tttcgcatgc gtcgggcgtg cccatgtggc
     7741 tgccatgcac caataacagc acttcgccgc tgcccttgtc gaaatagcgg gtcctgattc
     7801 gatcaacatc gataaacttc gggtcgttca tcattgagtg tatcctcgaa gaattaaaaa
     7861 gcgtgtacgt gatcggagga aggccgcgtg cgttgcgagc gcatgtcagc tgtattgtct
     7921 ccgaatctgt cctctctccg ttccgtttcc gctttcagcc ttccgcgttg tctttcccgc
     7981 gaccacgtcc tttttccgga cacgatcacg ttaattatcc cttcagcgac tcggcgagct
     8041 cgcgtaaata ctcttcctgt tctttggcct gatacagcgc catcgagccg ttagttttta
     8101 gcggtatgcc gccgatgaag tgaaaagcgt gggcgtccca gtcgtagaat tcgtcgcctc
     8161 ggtcgcagat ctctttccac ttacggccca cgggactgcg gtgcaagcct gtgcgcgccg
     8221 gaagcgcctc ttgctgctcg atggacaggt ccgcatttgc tttgtccgtc ttttcttccg
     8281 gaagtccgtc ccacatgcgc cggtagttgg gaaaaaattc ctcttcccgt accgtcaggg
     8341 cggtgtgcat gtcccaccat gggccgtgcc cggcgacacg ttcgtcgacc ttatccacca
     8401 atttgtaaat tagccgggcg cccgcgcgta aacgaagcac cagcgatccc tcggcggttt
     8461 tggaattctg ttcagcttgt atgccgcaat atttctcaaa aaatgccgcg gttctggcca
     8521 ggtcgcggca accatagaca gcatgactga tgcggccgac tttttcgtcg gtggcgattt
     8581 ccatggcgcc ttcgggcata tgcatgggcg cccagaattc atactgattg ccgtcagggt
     8641 cggcgaagta gattgccgtg ccttcttcgc cttcggcggc ggtcttcacc ggatcggtgt
     8701 gcgaaatttc gtagcgatcg agccggcgca ggtgcccatc gatatcttcc ggacggatat
     8761 agaagccgca acggggcagt tcttcgccaa gttcttttgc cgccggatag atttcgggct
     8821 gaaggaaggc gccgatgcgg cacgatccgc cctcccacca gagaaagcag ataattttga
     8881 tattcttttt acgcgcctcg gtagtcagtc ccctaatggg tcttcggata agcccgatgc
     8941 cgttcaccgc ccagtcagtc caggcgacca tatcgttggt gccgacgaca aaatgatcga
     9001 tccagtgagc cgcgcctttg ggttcgacgt tatccatcgt aaactcctca aggtctattc
     9061 acgatatttg aaacttccag tcttccgagc tcccttggtt gtcctccccg atagcggggc
     9121 gaaaaaaggt agcgattaac agccttcaat tccgcagatc acatcatcgc gaccgtacga
     9181 aacagggaaa attctgtcaa gcgcgataac caggacgccg cccgatttgt cactggaact
     9241 aggcgctgcg atggagattt tggtagagcg atgagaaatg caaatgcacg gatttaaggt
     9301 tcctacgatt tagccacgaa gaaataaacg gaagaagccc tccgcgacca tcggaagaat
     9361 gttctgttca caactcgccg gagtattgag ccgctacttc gaaccggctc ctgcggaaaa
     9421 gtattccacg cggctgaccg caacgtctga aacgaacatg actcccgtat agcggtcgaa
     9481 cagaggtttc aatccagcca aaatcggttc gactttctcc ggcgcgacga ctgacatcaa
     9541 catgacgatg ctgtctatct cgttgaacat cggatgggcg gtgtgaaaac cgtggtgccc
     9601 ttttccggaa atattgtgga tgatcgtgta tccagtggcc ttgttgtcga gcaagtcggt
     9661 ggcaaatttc aagtgctcac cctggatgat gatcctgatc tccttcattg gatgaagcgt
     9721 gagattgctc atgctgatct ccttctacac gaatctttgg cgcggggacg aactgctggg
     9781 tttcacggcc tgccacccct ctttcgaaac ataacgatag gctagctgct cttcaggctc
     9841 gatggcgatg agatacaacc attcattgtc atacagtcgc ttcagatggc gctggttgcg
     9901 gataatttta tcgatccttt ccctgggcgc ctccaccaga gtcacgagcc gcattggctc
     9961 atgataaggc cggattccat tcatcaccgt ctgcgaggcc aagccggtgc gcagatcgct
    10021 ctgaggaccg gacattattc caaaacggcc tactacgttg tggtagattt tgctgccgct
    10081 cccgtagttt tccggatcca cagcggagaa gtaatgctcc atattgatcc attgtccgac
    10141 aatttgtggt ccggtcatca cgacctcgag caattgcccg tcaaaatctt cgcggtaatc
    10201 gtaagaatgc aaaaaagctc gtccatcgag attgatgccg cgggtcagag cgcggcgccc
    10261 gatgataaat gcggcattgc ccgacagccc ccattccggc cgcacctggc tccagtcggc
    10321 gcttcggcga tgcaccgttc gccgggcttt cgaagccggc aggctcgttt tggtctcggg
    10381 aaagcgggcg catcgctctt ggctgttgag cgttcctgcc gattcaagat cgcgcacgag
    10441 ttggagcagg tccttgcggt gggtcggagg gagatcttcg agatcgatga gatcgacgtc
    10501 atctgtggtg gtattgtgtt gtccggcgac gaagtaggtg tcctgtggaa tgacaatgcc
    10561 gttcttggcg agctgttccc gcaccagcgg tttgttcgcc atgatcgcca gcaccctggc
    10621 gttaggcttg cctgggttac cgccacaggc gccgcagtcg agggccgctt cgaacgggtt
    10681 gttttcagag gtgctgccat gcccgcaaaa aagcactaga cgggcgaagt tccgggtcag
    10741 gcccataatc ctcagcgcgg tttccacagt gaagacttgc tcgctcagag tgaacccggt
    10801 gcgagtgatg cgctccatgc gagcaaagga tgcgccgggg ttaatgccgt agtttcgcct
    10861 gagatcttcg acgaaagccg cttcttcctc gcgggaaagc gcggtggagc gcgcctgcgg
    10921 cgatggttct cccgcgctct cgctgtagag cgcgcgccgg cgcagaaact ccaagcgttc
    10981 caatgataaa ttgagatggc gctcaccaaa gttttcctgc agcgcgcgcc gcgtgaccgc
    11041 ccgctgctcg gccgcaagca tttcttctac ttcttcccgc gatagtttgt cgacagtcaa
    11101 ggttgtggcg accggcggga ccagcttgcg ccgaagccaa ctcatgacct ttcgatacca
    11161 tgtcggcgaa agagtcttgc cgatgaacgg caggctgtaa aaccacccca gcgactcgac
    11221 catgacatag ggcgttacga cgttctcttt aagatcgtag agcagggtat ggccggtttc
    11281 gagaagcttt tctcgatgcc ggcgcctgaa taaatattga ccttgatagg tccgcggaat
    11341 ctcgcggacc aaattcttgc tcttcatgat aaccggaaac tgatcggtta catggtcgct
    11401 gccgaggcct tgatagcgga tgaagacggc aaaaaaaccc gcgaagccga aggtctcgta
    11461 atcgccaatc gcttccaagt gacgccggat cggctctgaa cgcacgtcga tgcaaaagac
    11521 cgcctgggct tggtgacgaa cttttgtctc gcgctgtgat tcgctttctg ccgcggctcg
    11581 atgggacgag ctcggaccgg aagcggcttc gccgggttga tcgagattca cagcgagctt
    11641 gcctaatagt tgttcctggt atgccgcctc gaacgcttta agccatacgg ggccatggtc
    11701 cgcctcggga aatccatcga tccagtcgag cagtgtcagg agattgccgg gctgactttg
    11761 cgttagaata acgggatcat gccccagggc ttgggcaagc gcgtacaagc gccaggccgc
    11821 gacaagagcg gccaggctct cagccggtgg ctggccggaa tgttttcgag ataagtcctt
    11881 gcgccgctgc agcgcggtcg atatcgcatc gaggttgccg tcgatgccga gcgattcacg
    11941 acaagctttt tgcaccagct cacgctcgta ccagaggcgt accgcaagat actgcgccag
    12001 atcgatgggg taggcgagct gccattcata ttcgctttga tcgccgcgcc atttgatgaa
    12061 gcccgcccat cccgacagag caacgaggtg aagcgaaaaa taatcctgcc gcgcatcggc
    12121 cggaatgccg agggttgcca agctttccag cagcgcgtct tccggctgag ccggcaatcg
    12181 ggcgagcttc tcgcgactat tttcgattgc tccgggagac cattcctttt gagcgagaaa
    12241 tttccaggcg ccgtagaagc cctgctcgcg gcgcggcatc ggccaggtcg catgaccttc
    12301 atccaagaag gcctcgcacc atttcaccaa ttcacgattg atttgttcga cgattgctgt
    12361 gcccagggtg cggtcgcacc aggtggccag cgtcagagtt cgaccgagat ctcgctgctc
    12421 tgcggtatcc cggccgctct gcggcggctt tgcggcggat actagatggc ttgcaagcga
    12481 tttgatcgtg ctccggtccg gatggcgctt cagcagagcg tcgaacgtgt tgagagcagg
    12541 aggagagagg tcttgcagca tgcaggcgcg aaggacagcg ccaagggtca ggttatgttc
    12601 gcctagttgg acttgcctgt tttctgcgcg ccctctcagc gcgagatcga gatgatcggg
    12661 cagaatccgg cccgagcaaa aatagtcgcg aaaaatttca accggcaaat accttttggc
    12721 ctcgatcaag cgctgcgccc gagaaacggc ctcttcaaaa tggagatact cgaggccatg
    12781 tagcggattg tggtgaacga atgtccgcat cggccaataa tcggcaatga cctcgctcgc
    12841 gagcaggatc aaggcccgga gctccatccg ctgggtttcg ctgtactgag ctgctgccat
    12901 ctccgcctgg ttcatctcca tggcattcga ccgacatggg ccagtacaac ggtttccaac
    12961 caatcatgcg gcatgacgcc cagagcaatt agaatcagca gcagaagcgc aaaacccgcg
    13021 acctcgctca cctcgagatc ggtgtagcga gtgtcggagc ggtgcggtcc aaacagaagc
    13081 tgctgcatca ttctgaacaa ataccatgat gctaaaaacc aactgaatag aactaccatg
    13141 agaccccacg agagggcgat ggaagcctgc accaacatcg ccagataggc ggaaaaaagc
    13201 cccaacggaa gcaggccgac cgccgccata atcaacaagg aaagcacggt ggcaaagcga
    13261 ggcatcgggg ttgccagacc gtgcatcgcc tcgaggctca gattgccgta ccgtgcacgc
    13321 acagtctgcc atgccagcag caggccagca gaaatcaaaa ctgtcgttgc gcagtagatg
    13381 atcggctgaa aagcaaaggt tccggccacg ccgaaactcc accacagagc tgaaaagaaa
    13441 gcgacgccgg cgtaagcaac aacctgggga actctcgttt gagccaaggc tttgaacgat
    13501 ccatacaccg cgccgaacaa agccagcacg cgcaccgcgc gaagagtctc ggccggaaga
    13561 tccggcatca actgagctat tgcgaacagt cccgccgccg gcagcagcac cgccagggcg
    13621 attgcctgat atccttgcca gcgcgtgagc gcagccacgt agattccgtg cagtggaaaa
    13681 agcggcagca gaatggcgac caagattgaa agtccgattt ctgccggaac aaccggcagc
    13741 cgcccgccca aggtcactgc cgagccaatt gcgatcaaag ctgccaggta cgaaaagatt
    13801 ctgctcccat tcaggcggtc gatttcacgt ctcaagaccc ggttaaatct tatggcaact
    13861 gcatcgaggt aaagccggtt catcaaaagc aggtaaatgc gaattcgtaa gctattcacc
    13921 cattcgggca tgccgatcga tcgcccatgg ctctccgcat aaatcagtac ccacccgaga
    13981 atgatgacca aggcgaaggc cgataccaaa atatcgaaca ctaggaccgg taaagcgccc
    14041 gcttggaaat ggacggccac ctcacctcgc gccggaaata ggaagtgtgt gaagctttcc
    14101 gcggccagca aataggtgac gacgaccaga aagagtgtaa agagcatact cgccgccacc
    14161 ttccaactag caaccgcccg caaccgatag agcgtcagga tcgcttggga agaagtcgcc
    14221 catccgaaga agagaaagat cgtcgttccc tgtgagtctc ccactgggat gcgcagaatc
    14281 ccatgagcag ccagcaggat catcaatggg agaatcaacg ttgtgatgaa tccggtaacc
    14341 caggtcagcg tcgagaactg acgcctctct attgccttag ccttgggggg cagtctgggt
    14401 tcctggcgag cggcatggat cacgtagccg caatttaaaa agatcgtggc cttaaacaaa
    14461 ccatgcgcca tcaagtgaaa gattgccagg ccgtaggcgc caagaccgca ctccatgatc
    14521 ataaagccca tctggccgat ggttgagtag cccaaggtct tcttgatgtc attctgaacc
    14581 agcatcatgg aagaacccaa aatcgtcgtg agcaagccga cggcgaagac tagatgcagg
    14641 gtcccaggat tgaggtcata gagcggcgcc agacgggtta agagaaaacc gcctgcattg
    14701 atgattcccg cgtgcagcag cgcggtgacg ggcgtcgggg catacagaga gtcgggcaac
    14761 cacatgtgca gtggaaactg cgccgacttg gtcatggcgc cgatgaaaag caacaaagtt
    14821 acggcggtgt cggcgcgcat ctcgaatcta tcgcctgggc cgagaaacag cgaggtttga
    14881 acctcggcgg cgcgagagaa cagctcttga aactccaaag tgccgtagga gccataagcg
    14941 agcaggatcg cagcgagaaa ggcaacgtcg ccgatgcgct gcatgataaa tgttctaaag
    15001 gcgccgcgaa ccgtcagagg atggctatga ttgaaagata ggagcgggac gagccaactc
    15061 agcagctgcc agaatataaa gagtattaac agattggcgc tcgagaccat gccgaacagc
    15121 gcgaatgtgg tgaaagcgag taagctgtga aatcgggtat atccgcgctc ctgttgcatg
    15181 tagcggatgg aaaatagatg gatcaggatg ctgatggtcg cgatgagcac gagcatcacg
    15241 gacgaaagcc ggtcgatgtg caagccgaat tgtaagatgc cgtcgctcca agaaaataaa
    15301 ccgatatttt gcggcccgcg gctggttact tcgtaaagga ccagcaagga aagcgtgaaa
    15361 gagatcactt ccgccgcgac gccgagccgg acagccttag ggcccatggc gcgccccaaa
    15421 aggccgatga tcaaccccgc cgccaacgga aagaagagaa tcaatgaagg gtaataagcc
    15481 aacataatct cggtctagaa acctgtgagc atagcttgat caaccaaaga tcgccgtctc
    15541 gtgcagaatt cattggcgat cgcaaaccaa aaaaagccgg cccttgaaag agccggccat
    15601 ctcgtaccga tccaatctgt gtgcttcacg ctatccgtag aatttttaaa ccatagcgta
    15661 tgaatttata gcagtaaagg catcgacatc cgacaaagaa ttacgctggc ggcctagtgc
    15721 cagtgctaga gcctagccgc tgcttccatc aaggctcgac catgaccttg atcgacttac
    15781 gctggtccat cgcccgataa ccatcaggca cttcgtcgag acctacgacc cggtcgaaga
    15841 cccggccagg ctcgatcctg ccctctatta cgtccggcaa gagctcctcg atgtaggcgc
    15901 ggattggggc gggcccgccg ctcacagtta cgttgttata aaaggctggg agcgaagccg
    15961 gcagagtttc accctgagga acgccgacgc ggccgactgc accccccgga cgcgcgatgc
    16021 tgatcgccgt gcgcatcgac tcttcgacgc cgacgcattc gagcaccgaa tgaacgccga
    16081 agccgcgcgt gagctcgcgc actcgcgcga ttgcctcctc gctgcgctcg ctcacgacgt
    16141 ccgttgcacc gaactctcta gcgagtgcga tccgatcggc atgccggccg agaatgatga
    16201 tctgctcggt tcctaaacgc ttcgcggcga tcacgccgca caacccgaca gcgccatcgc
    16261 caacaacggc ggcgactttt cccggaccga ctttcgcggc aacagcggca tgatgacctg
    16321 tacccatcac gtcagaaaga gtgagaaggg aaggcatcaa tgcgtcatct tgccctacag
    16381 gcagaacgaa gagtgttccg tcggccagag gcacgcgcag cgcttcggcc tgtgctccat
    16441 tcatgccagc tatgccgaag aaccctccct gaacacagga tgtctgcagt ccttcgtggc
    16501 agaacgtgca cacgccgtcg gaataggcaa acggcatgac gacaagatcg ccggctttca
    16561 tagtgcgaac ctcggcaccg atggcttgaa cgacgccgat ggcctcatgt cccatgcggc
    16621 ggccgccccc cgggatctct tccattcctc gatacggcca taaatcgctg ccgcagatac
    16681 aagcccggct gaccgctacg acgacgtcgg tgggctccac gatatcggca tcgggaacat
    16741 cctcgatacg aacgtcgccg gcgctgtaca tgatagttgc acgcataatg attatctctc
    16801 gtttccgggg ggctgagccg tttgatattg ttcgtcgctg accttctcca tccattcgac
    16861 gaccttaccg tcgagcgctt cctgaatggc gatatgagtc atcgctgtgg tcgtcgtagc
    16921 gccatgccaa tgcttctcct tcggcgggca tgaaaccaca tcgcccggtc gtatttccac
    16981 ttttggatcg ccttcgcttt gcacccatcc gcagccggct gtgacgatca aggtctggcc
    17041 aagcggatgt gtgtgccagg ccgaccgggc gccgggctcg aaggtgacaa ttgcgccgcg
    17101 cgcccgtgcc ggctcgggcg cctcaaacag aggatcaatc cgtacacagc cgg
//
LOCUS       DASUVS010000267        21309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1856988, whole genome shotgun sequence.
ACCESSION   DASUVS010000267 DASUVS010000000
VERSION     DASUVS010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 21309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21309
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856988"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VF182_14600"
     CDS             complement(<1..945)
                     /locus_tag="VF182_14600"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX6438364.1"
                     /translation="MRLTPPVGTEVVEVGNDLPSRAMSRADPFISGIITPEEGWLLQR
                     EKLLAWLRLAFAIVAIAVVQLNPSRTIRFPTLSVFSLWSFLIYSLLILELVRRQKITS
                     KKIGIITACLDSVWIFLIVFSTGGTRTPFFFYFSFPILSASLRWGIKGSVLVALVVVG
                     LYGLIRFSMAAESFERPMGIDTLLVRSIYLITLAYIFGFISDFEKKQNQRLLALSDTA
                     IKAAAIQERRRITYEIHDGILQSLATIILRLESCRSRLLDSQDELYRDIEGIENFTRT
                     SMTEIRNFLAGKDTQPFTPGTLIEKLREEMKFLQESLGL"
     gene            complement(899..1603)
                     /locus_tag="VF182_14605"
     CDS             complement(899..1603)
                     /locus_tag="VF182_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6438365.1"
                     /translation="MMPIKVVIADDHALFRDGLRRILSLEKDILVVGEAANGDDVIKA
                     VERTKPDILLLDLKMPKGDVVQNLLEVGAKYPSTRVIILTAFCEEESVLNAAKGGAKG
                     FVPKGVPSTTLLQAIKAVHHGASWTDKEIPAWEAFEEIAQGQSNAREPPTQFDESIRS
                     LTKREMEILRLVAEGLTNEEIGKKIFISEKTVKTHLTNIFDKLKVNNRFKAALMLMGQ
                     SGETNTASWHRSGRGR"
     gene            1822..2292
                     /locus_tag="VF182_14610"
     CDS             1822..2292
                     /locus_tag="VF182_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438366.1"
                     /translation="MKNLFSIGNGAAAVMVAFAVSSCSTQEEMASKPAAAPAASPAKA
                     EAKAGSKATGDDATAKAESKGEAKGEAKAKVEKARGDVGLLDTEKNYLILVTKEGKLI
                     TLDYDAKTKATQIDPTPAKMADVGLGSSATVEYTKQGDKNIAKNVEFTPAKGGD"
     gene            2345..2734
                     /locus_tag="VF182_14615"
     CDS             2345..2734
                     /locus_tag="VF182_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438367.1"
                     /translation="MKMKSEIVSALSIMCALSLASPIVAQEGEKKAEGAKKAEGEKAE
                     KAKAEKVSGDIGLLDTEKNYMIVVTKEGKLVTVDFDQKTKVTELKAKDAKMADIGLGS
                     SATVQYTKKGEKNYVSGIEFTPAKGGD"
     gene            complement(2836..3294)
                     /locus_tag="VF182_14620"
     CDS             complement(2836..3294)
                     /locus_tag="VF182_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX6438368.1"
                     /translation="MSKSENSKDAKARKEFLKSAAETLLEMKKQLHKEMQVRVKGETD
                     GVKDEGRDTYDLASDERDREINFILNDREREKLHAIDEALERIKDKSYGICESCEGEI
                     QLGRLKVLPFTRLCVRCQEENEKESKRQKTLEEERGYRKLVLTDFEEEGS"
     gene            complement(3287..4627)
                     /gene="rimO"
                     /locus_tag="VF182_14625"
     CDS             complement(3287..4627)
                     /gene="rimO"
                     /locus_tag="VF182_14625"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX6438369.1"
                     /translation="MNQPKQKFSIISLGCARNLVDSEVMSGLLGQDQYQMVQEPADAD
                     VVLINTCGFIDTAKAESIDTIVEISRLKQEGRLRKLVVAGCLSQRYPQELATELPEVD
                     LFIGTGEVPRIVSILQDHEQQNRRHYVGVPEYLYDHVTPRLRSTPSYTAFLKVSEGCD
                     HKCAFCIIPKLRGPHRSRSIESVIKEATMLAQSGVKEINLIAQDLTAYGRDRKDGTTL
                     YGLLKELVHVPDLVWIRLLYAYPNFLDSSLLELINTSEKICKYIDIPFQHVSASILKS
                     MRRGKSGSAVAVAVEKLREAIYGLTLRTSLIVGFPGETEEDFEALLDFVERTRFERLG
                     VFKYSIEEGTAAASLPDHVDEEEKEQRWQEVMDLQANISRLKNEALIGSMQRVMIDGV
                     DETGQLIGRTQGHAPEVDGAVYLHNAASLGSGDFADVKITEALEYDLIGEIQHV"
     gene            complement(4624..5286)
                     /locus_tag="VF182_14630"
     CDS             complement(4624..5286)
                     /locus_tag="VF182_14630"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX6438370.1"
                     /translation="MKPKRLFGALLIALSLVAHDWSIAAESKSAEQIVDALQKNYDAT
                     VDFVADFQQETEVKSLNRSLKAWGKLSFKRPGKMLWRYDEPKGQIVLTDGSHLYFLQP
                     EQNQVIKSPLKNAFRSDIPLSFLLGLGNLKKEFNAVLKATENDQYILRLEPKGEAGGF
                     SEILMGVSRSSADIAWVSIRDAANNLTSLRFSNMKKGVGLQESLFRLKIPEGVDIVEL
                     GQ"
     gene            complement(5276..7519)
                     /locus_tag="VF182_14635"
     CDS             complement(5276..7519)
                     /locus_tag="VF182_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX6438371.1"
                     /translation="MEKGRLNREVWGVVIGLTALLMIISLVSYNPNDRSLNTPSGLIA
                     HNWGGFVGAFLADLLLQGLGLAAYFLPIFLGVAAYHLFLPSFKGLRAAKAVGYTVLLI
                     GITVLLSLLIDSENARDAGGIVGGFLTESVLVPLFGRISATLILLAALLLAIMTVSQI
                     SLADLLDRSGKRLLQFRKFIVPKITVGVAELKQQKDKPKGENAKTERRDYVPPPIVVK
                     ADPKEELIKKAVKKPTVPHEQFKLPVVGEGYRLPPLELLDPPDPSQLIKVDTNSLHAN
                     SLILQKKLADFGVDGEVVAVRPGPVITMYEFKPADGVKVRRIVMLADDLAMALRAVSV
                     RILAPIPGESVVGIEIPNPRRETVYLREIIESDSYNSVDSKITLALGKDIGGIPFATD
                     LGRMPHLLVAGATGTGKSVSINAMILSILFKATPQDVRFIMVDPKMLELTVYEEIPHL
                     LVPVVTDPKKAAAALFWAMDEMDRRYRLMRDKGARNIDNYNRTLEKEGAVKKPVIELT
                     EAQAEEDPGAPGGSLAKEEPLVHERLPRIVIIIDELADLMMTVGRDIEEYITRLAQKA
                     RAAGIHLILATQRPSVDVITGLIKANFPARISFQVTSRIDSRTILDSMGGEKLLGNGD
                     LLFLPPGTARLTRIHGAFVSDQEVRKVMKFIKQQGRPQYRPEVFEIKKDIDNAGADEE
                     YDEMYDQAVAIVTDTQQASISMIQRRLRVGYNRAARMIEQMERDGVIGPADGAKPREV
                     YARKLEA"
     gene            complement(7535..9190)
                     /locus_tag="VF182_14640"
     CDS             complement(7535..9190)
                     /locus_tag="VF182_14640"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX6438372.1"
                     /translation="MESVRIIPLGGLGEFGLNMMLVEYGEAAIAIDCGLMFPGADLLG
                     IDLVIPDISYLLENKHKLKAIVLTHAHEDHMGAIPYVLERLSVPIYATRLTLGLLSNR
                     VREHGLEDVADVREITAGKLWEVGPFKLEGIRVTHSLMDCLALAIQTPVGTVIHTGDF
                     KIDNTPMEGEMFDFQRFAAYGEKDVLLLLSDSTNVERRGHTGSEREVGTNLEHIFQQS
                     NGRVLVSTFASSIPRIQQVLEISERCGRRVVLSGRSMVRNSQVASDLGYLRLPQSFMT
                     ENDRWQDLAPDRVTFLTTGSQGEPLSVLHRVALNDHKTIKIQPGDNVILSSKFIPGNE
                     KTISNLINHLYRRGAEVHYEKVSEIHVSGHASQEELKTMLQLTRPRFFVPIHGEYRHL
                     VRHRRLAQEVGVPENNCFILEDGDVLELTAQSAHKTTPVQVGKVFIDGKGVGDVGPIV
                     IRDRRHLSEDGMVLAVLAIHQQSGEIVAGPDLISRGFVRDEEGEEILEQARKVVLETF
                     SGMNRETRADRAELQEEIRVALRRYFRKKLERRPVVLPYIVET"
     gene            complement(9196..9978)
                     /locus_tag="VF182_14645"
     CDS             complement(9196..9978)
                     /locus_tag="VF182_14645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6438373.1"
                     /translation="MFYLLKLCVFMIATVPAALVTIVLGLFDRHGKSAYRASQIWTAL
                     ILRMGGIALRVSGREYIDSRQPYVFMVNHQSNLDIPVLVQSLVNFQLRWIAKRELLWI
                     PFFGWAMWASRHITIDRGDPLAAIKSLRRAKERMAAGISIVVFPEGTRSRDGKLLRFK
                     KGGFLLAAQSNRPIVPVTINGSRQVLPAGAWRLRGGTVDVIIGEPIAVDGFGPGTLRH
                     LSNQVRQAIEKNLRSTPVDLDSVTANSRHGIISDRHLEKRSI"
     gene            10165..10593
                     /locus_tag="VF182_14650"
     CDS             10165..10593
                     /locus_tag="VF182_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX6438374.1"
                     /translation="MSVNKVILIGNLGKDPELRFTSTGSAVARFPIATSEVWNDRDGN
                     KQERTEWHNIIVWGKQGESCSQYLAKGRQVFVEGSIRSRSYDDKSGTKRYVTEIVAQR
                     IRFLGGGGGTRMAPEIEAGGANDEISTAGGQPPFDDDIPF"
     gene            complement(10603..11580)
                     /locus_tag="VF182_14655"
     CDS             complement(10603..11580)
                     /locus_tag="VF182_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX6438375.1"
                     /translation="MTMTLPKIVFLDAATFGDVSLARFSEIGHCRIYQVTQPGETIGR
                     LAGQSIVVTNKRSIDQAALAAPESRNLSLIAVAATGTDIIDHTAARQRGIKVCNVPGY
                     AAQSVAQFTMALILELATHAGRYASDVKAGMWQKSPIFSLLTYPIVELNGKTLAIIGY
                     GNIGREVGRMARSFGMEVVVAARPGAAEPHEPDRVPLEQLLPRADIVSLHCALTPQTK
                     NLIDGEMLKLMKPSALLINTARGALIDESALIQALREQRLAAAALDVISEEPPASDHP
                     IILAASALDNLLVTPHTAWSARQARERLLDEVAENIAAFLAGRDRNRVA"
     gene            complement(11577..12386)
                     /gene="mazG"
                     /locus_tag="VF182_14660"
     CDS             complement(11577..12386)
                     /gene="mazG"
                     /locus_tag="VF182_14660"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX6438376.1"
                     /translation="MFDHKKAGEKFSEVLELMARMRGENGCPWVREQAHGSLKPFLLE
                     ESHELLDALTGNDPAKIAEELSDLLYQIVFHCQIGAENGTFTAGEVISNLAAKIVRRH
                     PHVFGGDALPDSEAVTKQWAQIKGQEKPAGNTNSALGSLPKSMPALARAQTISERAAR
                     VGFDWPNIEPVWQKVEEELSELKNAWASGDRQRAGEELGDVFFSLVNLARFLVVQAED
                     VASQTIDRFIERFHYLENQLRAAGKTPDQSSLEEMDRLWEEAKALERRGRL"
     gene            12524..13372
                     /locus_tag="VF182_14665"
     CDS             12524..13372
                     /locus_tag="VF182_14665"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6438377.1"
                     /translation="MSTATIDGLEVRYEVFGSGAPLLLFAPGGFDATIEKWRAASAWT
                     GIDALQTLATRFRVIAYDRREAGQSGGRVERLTWHTYARQAKALLDHLKIESALVLGG
                     CMGCSVALAFAVDYSQATRALLLHWPVGGFRWKAGGLDRFERHYRFASTHGLEGVVER
                     ARTGKSFWTDPEAGPWASVIAQDPSFAEDFVRQDLHRYLGLTAVSGRTLFDRDTATGA
                     EPEELMGISAPALIVPGDDASHATSAAHYLRELLPNASFWNVLPPEQTTRAVCDRILE
                     FSRQSS"
     gene            13478..14197
                     /locus_tag="VF182_14670"
     CDS             13478..14197
                     /locus_tag="VF182_14670"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6438378.1"
                     /translation="MDVTTSTLQLSTADGNMDAFEARPKDGGSYPGIVVLMEAFGLND
                     HIKKVTERIAQEGYVAIAPDLYHRESERIIPYSDMKKAIGTMNRLQDPKVMDDVGAAI
                     AHLKGQSNVKGGALGVTGFCMGGRYTYLAAAHHNKDVKAAVCFYGGGIPMGEPSPLSR
                     TGEITCPIYLFFGAKDPLIPMDQVGQINTELTHKKVSFSMKVYPEATHGFFCDERQSY
                     HESAAKDSWEKLKTFFAQHLK"
     gene            14280..15176
                     /locus_tag="VF182_14675"
     CDS             14280..15176
                     /locus_tag="VF182_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HEX6438379.1"
                     /translation="MPVNKNKDIVTVGLVQMSASEDPEANLSKAEARIREGARKGAQI
                     VCLQELFRSRYFCQSEDQRCFALAETIPGPSTERLSALARELQIVIVASLFEKRSAGI
                     YHNTAVVFDADGSIAGKYRKMHIPDDPLYFEKFYFTPGDLGFPSFQTRYARVGALVCW
                     DQWFPEAARLTSLSGAEILVYPTAIGWIPKEPRAVAQTQRNAWELIQRAHAIANGVFV
                     ASVNRVGREGNLKFWGHSFVAGPSGEILSVAAGDREEILIAKCDLRKIDEIRESWPFF
                     RDRRIDAYSSLQSRFIDSPDGT"
     gene            15202..16278
                     /locus_tag="VF182_14680"
     CDS             15202..16278
                     /locus_tag="VF182_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX6438380.1"
                     /translation="MADQDKPSQMPRDLGYSMPAEWTPHRATWLSWPHNQETWPAQLE
                     RVREAWVQMILALSPHEKVCLLVNDDAMRQHVSSRLKTAGAVMENVTLIEIPTVDVWM
                     RDYGPTFVVRSRAAVSLALNDWIFNGWGGKYKAYEEDDEVAKAIAQLLGVPVFAHALV
                     LEGGAIEVNGAGICLTTEQCLLNNNRNPHLSRAEIEQFLRESLGVSQIIWLGEGVAGD
                     DTDGHIDDIARFADTTTIVCAVEGDPQDANYVPLRENLERLQQARDQHGSRFDVVTLP
                     SPRPVICQGSRLPASYANFYIANEVVLVPLFDDPGDQNALGTLGELFSTRKIVGIRCT
                     DVVAGLGAIHCVTQQEPKIEGSRA"
     gene            16543..17670
                     /locus_tag="VF182_14685"
     CDS             16543..17670
                     /locus_tag="VF182_14685"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX6438381.1"
                     /translation="MELTKSLPLVLLLLVGLTILGWFFLCRMRSLLRATQNEQSWLLM
                     QQQIDQLRVQFGQVLDNSTQLIQQQLGQMIGHVNERLRENSEILQQTHQSVGQRLDNA
                     ARVVGDVQKSLGGLEEANRKIYEVGKDIASLQEILRSPKLRGGLGEFFLEDLLAQVLP
                     PQHFTTQYCFRSGEKVDAVIRLGSSLVPVDSKFPLENFKRIIDTKVDDEQARAKRQFA
                     ADVRKHIDAIATKYILPDEGTYDFALMYIPAENVYYEIIIKGDSNEEKSLSTYAMSKR
                     VVPVSPNCFYAYLQAIVLGLKGMTIEQRAKEIIECLSRLQGDFTRFRDEFGLIGKHLG
                     HAQSSYQAAEKRFEQFGQKLVSANAGQTELFQTAAADRKPL"
     gene            17700..19142
                     /locus_tag="VF182_14690"
     CDS             17700..19142
                     /locus_tag="VF182_14690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6438382.1"
                     /translation="MQLSLFSRLALGYLAIFLLVTAVSTYAVFQLHRVDELTDSVLKV
                     DNRILDYEKILADLLLSQSRYEQKFAISRDDALYDEFLSLRRDFNVQLATAITIGDSA
                     AGAILSKVQQDYRNYEELVAKEAALLRAKKSYPQAQFKQQKDAIVDDILVSLEKLRAD
                     QQYAMSTKVKELAEAAEHAREVSVTITVACLISIVVMSLIVTRSITRPIAILKTKTRE
                     IAQGRFEGGLNIKSPPELGELAAAFNLMCERLKELERMKADFFSSMSHELRTPLTSIK
                     EGTGLLLDGVGGETTDKQRKLLAILAEESNRLIAVVNSLLDLSKMEAGMMSYEFDLVT
                     IEPLIRRAIAEITPLVEAKQIELKSSVNPSLPALRLDPERILQVLRNLLGNAVKFTPK
                     GGRVSVVAAAVNGNLQVSVTDSGPGVPAESLTTIFEKFSQGSHKGANTRNGTGLGLAI
                     AKNIIKSHGGEIWAESQLGRGSTFVFVLHC"
     gene            19208..19696
                     /locus_tag="VF182_14695"
     CDS             19208..19696
                     /locus_tag="VF182_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438383.1"
                     /translation="MLQGHSLFVNGDYDGAFAKYREALVLAGDHTPADAAVYNIGLIY
                     AHPNNPKRDNRKAMESFKKVISEYAQSPWAEQSKIWLDVLQQLEVAKQEAETTRQAME
                     QSKEAIERSREDAEKFRLAAEKSKQELDRTRQEMEKSRQALEKANQIDIEIEQKKRER
                     GK"
     gene            19711..21138
                     /locus_tag="VF182_14700"
     CDS             19711..21138
                     /locus_tag="VF182_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6438384.1"
                     /translation="MSLGKILIVDDDANLLELIKMRLESADYEVTASCEEDNALAALK
                     AETFDLCIVDLMLANRDGIMLMEEFKAVNPEVPTIILTAHGSIQNAVEAMRRGAYSYL
                     TKPFEPGDLLLQIERALENRKLTSEIKRLKGLLHEQFDFANIIARSAKMRSVLDVVMR
                     IARLDSTVHIHGESGTGKELIAKAIHLASDRKDKPFIALNCAALPETLLESELFGHEK
                     GAFTGAVKSTRGLFTQAHGGTLFLDEIGDMPLSTQSKLLRVLQERQFYPVGSEIPTEV
                     DVRVIAATNKDLEELVKKGTFRDDLFYRIHVIPIYLPPLREHKEDIVPLVDHFLKKCR
                     QQMKKDVKGLTPEALRKLMVHDWPGNVRELENTVEYSVAMTQKEFVTEEYILQGRMSF
                     SDGAQAVNDKPPVSDDALRPLKDARDAFERDYLVQVLSMTDGNVSQAAKLAGKYRADF
                     YDLLKKHDLKADDFKKTKSAVSAST"
BASE COUNT         5100 a         5573 c         5650 g         4986 t
ORIGIN      
        1 caagcctagg ctttcttgca gaaacttcat ttcttctcga agcttttcta tcagcgttcc
       61 cggcgtaaac ggctgggtgt ctttgccggc caaaaagttc cgaatttcgg tcatcgaagt
      121 tctggtaaaa ttctcgatgc cttcgatgtc ccggtataac tcgtcctggg aatcaagaag
      181 cctgctgcgg cagctttcga gtcttagaat gatggttgcc agagattgaa gaatgccgtc
      241 gtggatttcg taggtgattc tgcgtctctc ctgaatcgcc gcagccttga tcgccgtgtc
      301 cgaaagcgcg agtagccttt ggttctgctt cttctcaaaa tcactgataa aaccgaagat
      361 gtaagctaag gtaatcagat agatgctgcg cacgagcaag gtgtcgatgc ccatcggcct
      421 ctcaaagctt tccgccgcca tgctaaaccg tatcaaacca taaaggccga ccactaccaa
      481 agcgaccaat acgcttcctt tgattcccca gcgtaggctg gcagagagga tcgggaaaga
      541 gaagtagaaa aaaaacggtg tgcgggtgcc accggtggaa aatacgatca aaaaaatcca
      601 aaccgaatct aaacaggcgg taatgattcc gatcttcttg gaggttattt tttgccggcg
      661 aaccagctct aggataagaa ggctataaat tagaaacgac catagtgaaa agactgaaag
      721 cgtcggaaat cggatagtgc gtgacggatt aagctgtact acggctatgg cgacgatggc
      781 gaaagctagc cgcagccacg ccaaaagctt ctctcgttgc aggagccagc cttcctcggg
      841 agtaatgatg ccagagatga atgggtcggc gcgggacatg gctctcgaag ggagatcgtt
      901 accgacctcg accacttcgg tgccaactgg cggtgttagt ctcacccgat tggcccatca
      961 gcattagagc agccttgaag cggttattaa cctttagttt gtcgaatata ttggtcaggt
     1021 gagttttgac tgtcttctca ctgatgaaaa ttttcttgcc gatttcctcg ttcgtaagcc
     1081 cttcggcgac caaccggagt atctccattt ctcgttttgt aaggcttcta atactctcgt
     1141 cgaactgagt cggcggttca cgcgcattgg actggccctg agcaatctcc tcgaaagctt
     1201 cccatgcggg tatctcctta tcggtccaag aagccccgtg gtgcaccgcc ttgattgctt
     1261 gaagcaaggt agtagagggc acccccttgg gaacgaatcc cttggcgcca cctttcgccg
     1321 catttagaac gctctcctcc tcgcagaagg ccgtcaagat aataacccgg gttgaagggt
     1381 attttgcgcc gacttccagc aaattttgaa ctacatcccc ctttggcatt ttcagatcca
     1441 aaagcaagat atccggcttt gtccgctcga cggctttgat cacatcgtcg ccgttggccg
     1501 cctcgccaac gacaaggata tccttctcaa gcgaaaggat tcgcctcagc ccatctctga
     1561 acagagcatg gtcatcagca attacgactt tgatcggcat cattgcattc gctcctagtg
     1621 aagattaccc atgcaagcgc aaaatacacg aaatgaatcg acatgccaac ttagtaatca
     1681 gggcttcttt actaagacta tagtttgatt tcccaagatt agtggccgac aaaaccatac
     1741 ttatagagta ttgagatgaa tcggcgctca cgataattgt caactctaat tttgctggca
     1801 aatatgttcc aggaggcaac gatgaagaat ttgttttcga ttggtaatgg tgcggctgcg
     1861 gtgatggtgg cttttgctgt ctcttcatgt tcgacacaag aagaaatggc ttccaagccc
     1921 gcggcggcac ccgctgcatc gccggccaaa gccgaagcca aagccggctc gaaggcgacc
     1981 ggtgatgatg ctacggcaaa ggccgagtcg aagggcgaag ctaagggtga agccaaagca
     2041 aaagtggaaa aggcgcgagg cgatgttggt ctgctcgata cggagaagaa ttatctcatc
     2101 cttgttacca aggaaggaaa gctgattaca ctggattacg acgccaaaac caaagcgacc
     2161 cagattgatc ctacacccgc caaaatggcg gacgtcggac ttggttcttc ggcaacggtc
     2221 gaatatacaa agcagggcga taaaaatatc gccaaaaacg tggaattcac cccagccaaa
     2281 ggcggggatt gatcctaatc aagtctcacg tctaattacc tacgtccagg aaggggatga
     2341 atttatgaag atgaaatccg aaattgttag cgctttgagt atcatgtgtg cactctcact
     2401 ggcctcgccg atcgtggccc aggaagggga gaagaaagca gagggtgcaa aaaaagcaga
     2461 aggggaaaag gcggaaaaag cgaaggcgga gaaagtttcc ggcgatatcg gcttattgga
     2521 tactgaaaaa aactacatga tcgtggttac caaggaaggc aagctggtaa ccgttgactt
     2581 tgaccagaag acgaaggtca cagaattaaa ggctaaggat gcaaaaatgg ccgatattgg
     2641 cttgggatca tcggcaacgg tgcagtacac caagaaaggc gagaaaaatt acgtaagcgg
     2701 gattgaattt acccctgcta agggcggcga ctgattagcc aaagcttgac gaaaaagagg
     2761 gcatggtttc caccatgccc tctttttttg cctgtgaagc caactctgga agttttatcc
     2821 ggaccggatc tgatgttagg agccctcttc ttcgaaatcg gtcaaaacaa gcttgcgata
     2881 gccccgttcc tcctcgagcg ttttctgacg tttgctttcc ttctcgtttt cttcctgaca
     2941 ccttacacaa agtcttgtga atggcagcac tttcaagcgt ccgagttgaa tctcaccttc
     3001 gcaactctcg cagatgccgt agctcttgtc cttaatacgc tcgagggctt cgtcaatcgc
     3061 gtgcagtttc tcccgctctc gatcattgag aataaaattg atttcccggt ccctttcatc
     3121 gctcgctaga tcataagtgt cgcgaccttc atccttaaca ccatcggttt cgccttttac
     3181 ccgaacctgc atttctttat gaagctgctt cttcatctcc aacaaagttt ccgcagcgct
     3241 tttgagaaat tcttttctgg ctttagcgtc cttggaattt tccgatttag acatgttgta
     3301 tttcaccaat caaatcatac tcaagagctt cagttatctt tacgtcggcg aaatcgccag
     3361 atcccagaga agctgcgttg tgcaagtata cggcgccgtc gacttccggc gcatgaccct
     3421 gtgtgcgccc aatcaactgc cctgtttcgt caacgccgtc gatcataacc cgctgcatag
     3481 acccaatcaa ggcctcattc ttgagtcgcg agatgtttgc ttgcaaatcc atcacctctt
     3541 gccagcgttg ttctttttct tcttcgtcga cgtgatcggg aaggcttgcc gcggcagtgc
     3601 cttcttcgat cgaatactta aagacgccga ggcgttcaaa tctggttctc tcaacgaaat
     3661 cgagcaatgc ttcgaaatcc tcctcagttt ccccgggaaa cccgacaatc agcgaggttc
     3721 gaagggttaa accataaatg gcttcgcgca acttttctac cgcaaccgct acggcactgc
     3781 cgcttttgcc tcggcgcatg cttttcaaga tgctagcgct gacatgctgg aaggggatat
     3841 caatgtactt gcagattttt tcactggtgt tgatcagctc gagcagcgag gaatcaagaa
     3901 agttcggata ggcgtagagg agccgtatcc atacgaggtc cggcacatgc accagttcct
     3961 ttaggagccc gtacagagtc gtaccgtcct ttcgatctct gccgtatgca gtgagatcct
     4021 gggcgataag atttatctcc ttcacgccgc tttgggccag catagtagct tctttgatca
     4081 cggattcgat ggaccggcta cgatgtggcc cgcgcaattt cggaatgata cagaaggcgc
     4141 acttatggtc gcatccttcc gaaaccttta gaaacgcagt gtacgaggga gtactgcgaa
     4201 gccgaggcgt cacatgatca tataggtact cgggaacacc gacatagtgt ctacggtttt
     4261 gctgctcatg gtcttgaaga atactgacta tccgcggaac ttcccctgtg ccgataaata
     4321 gatcgacctc gggcaactcg gttgctaatt cttgcgggta ccgttgcgag aggcatccgg
     4381 cgactaccag ctttcgcaat cttccttctt gtttcagtcg ggaaatttcg acgattgtgt
     4441 ctatcgactc cgctttcgcc gtatcgataa agccgcacgt attaatcagt acaacatccg
     4501 cgtccgccgg ctcctgaacc atttggtact gatcctgtcc cagaagtccg gacatcactt
     4561 ccgaatcaac cagattgcga gcgcatccta agctgataat gctaaacttc tgttttggtt
     4621 gattcattgg cctaactcga ctatgtcaac cccctcggga atttttaacc gaaacaatga
     4681 ttcctgaagt ccaacgcctt tcttcatatt ggaaaaacgt aagcttgtca gattgttggc
     4741 ggcgtcgcgg atgctcaccc aggcgatatc agcggagctc cggctcaccc ccatgagaat
     4801 ttcactaaag cctccggctt cgcctttcgg ttcaaggcgg agaatgtatt gatcgttttc
     4861 agtggctttg aggacggcat tgaactcctt tttcaagttc cccagtccaa gtagaaaaga
     4921 tagcgggata tctgagcgaa aggcattttt caatggactt ttaatcacct gattctgttc
     4981 aggttgtagg aaatacaggt ggcttccatc tgtcagtacg atttggccct tcggctcgtc
     5041 gtagcgccac agcattttgc ccggtcgttt aaacgacaac tttccccacg cctttaagct
     5101 gcggttaaga cttttaactt cggtttcctg ctggaaatcg gcgacgaaat cgacagtagc
     5161 atcatagttt ttttgcagcg cgtcgacgat ttgctcggcg cttttcgact ccgccgcgat
     5221 actccaatcg tgtgcgacca aggacaaggc gatcagcagc gccccaaaca gtcgcttagg
     5281 cttcaagttt tctcgcatac acttctctcg gcttggcgcc gtctgccggg ccaataacgc
     5341 catcgcgttc catctgctcg atcatccgcg cggcccggtt gtatcctacc cgcaatctcc
     5401 gttgaatcat agagatcgaa gcctgctgcg tatcggtgac gatagccacc gcttgatcat
     5461 acatttcgtc gtattcttcg tcggcaccgg cgttgtcgat atcttttttg atttcgaaaa
     5521 cttcgggccg atactgcggt cgtccctgct gtttgataaa cttcatgacc ttgcgcactt
     5581 cttgatccga tacgaaggct ccatggatgc gcgtcagtcg ggccgtgccc ggcggtaaaa
     5641 acagcaaatc tccgttgcct aaaagcttct caccgcccat cgagtcaagt attgtgcgcg
     5701 aatcaatgcg cgaggtcacc tgaaatgata tgcgcgccgg aaaattcgct ttaatcaaac
     5761 cagtaataac atcgaccgag gggcgctgcg tggcaaggat caggtggata cccgccgctc
     5821 gcgccttttg cgcaagtctg gtgatgtatt cttcgatatc gcgtccgacg gtcatcatca
     5881 aatcggccag ctcgtcgata ataataacaa tccgtggcaa acgctcgtgc accagcggct
     5941 cttcctttgc caggctcccg cccggtgcac ccggatcttc ctccgcttgc gcctcggtga
     6001 gctcaataac gggtttcttg acggcgcctt ctttttccag cgttcgattg taattatcga
     6061 tattgcgcgc gcctttatct ctcatgagac gataacgtcg atccatttcg tccatggccc
     6121 agaaaagcgc ggcggcagct ttttttggat ccgtaaccac aggcacgaga aggtggggaa
     6181 tttcttcgta cacggttagc tcgagcattt tcggatccac catgatgaag cgaacgtctt
     6241 gcggcgtcgc tttaaagaga atgctcagga tcatggcgtt gatcgagacc gacttgccgg
     6301 ttcccgtggc gccggcaacc agcagatgag gcatgcgccc tagatcggtg gcaaacggta
     6361 ttccgccaat gtctttcccc agcgccaagg tgatttttga atcgacggag ttatagctgt
     6421 cgctctcgat gatttctctt aagtaaaccg tctcacgccg gggattgggt atttctattc
     6481 caaccaccga ttctcccgga atcggcgcga gaatgcgcac gctgacggcg cgcagcgcca
     6541 tcgcgagatc gtcagccagc atgacgatac gtcggacctt tacgccatcg gccggcttaa
     6601 actcatacat cgtgatgacc ggaccagggc ggaccgctac cacttcgccg tcgacgccga
     6661 agtcagccaa ttttttttgc aggatgagag agttcgcgtg caagctgttg gtatcaacct
     6721 tgatcagctg gctgggatcg ggagggtcta acagttcgag aggcggcaat cggtaacctt
     6781 ctccaactac cggcagttta aactgctcgt gcgggacagt cggctttttg acggctttct
     6841 tgatcaattc ttccttagga tcagccttca ccacaatggg tggtggcaca taatcgcgcc
     6901 gttcggtttt ggcgttttct cccttgggtt tgtctttttg ctgtttgagt tcggctacgc
     6961 ccaccgtaat ttttggaaca ataaatttcc tgaactggag cagtcgtttg ccgctgcgat
     7021 caagcagatc cgccagcgat atctgtgaaa ctgtcatgat tgccagaagc aaagctgcca
     7081 ggagaatcaa agttgcactg atgcgtccga ataatggcac gagaacgctt tccgtgagaa
     7141 aacctccgac aattccgccc gcgtctcgtg cgttctccga atcgatcagc aggctcaaaa
     7201 gaacggttat gccgatgaga agcacggtat aaccaaccgc cttggcagcg cgaagccctt
     7261 tgaaactagg aagaaacagg tggtaggcag cgacgccaag gaaaatcggc agaaaatagg
     7321 cagcgagacc gagcccctgc aacaggagat cggcgagaaa ggcgccgaca aagcctcccc
     7381 aattgtgagc gatcaagccg gaaggcgtgt tgagcgaacg gtcgttggga ttataggaga
     7441 ctaaactaat gatcatcaga agagcggtca ggccaataac cacgccccag acttcccggt
     7501 taagccggcc tttttccatg gcagtagagc gaccttatgt ctcgactata tacggcaaaa
     7561 cgacaggccg ccgttccaat tttttgcgga aataacgacg tagcgcaacg cgaatctctt
     7621 cctgcagctc ggctcgatcg gcgcgggttt cacggttcat accactaaag gtttccaaaa
     7681 ccacttttcg agcctgctcc agaatctctt cgccttcttc atcacgtacg aatccgcgag
     7741 aaatcaagtc aggcccggca acgatttcgc cggactgctg gtggatggcc aaaaccgcaa
     7801 gcaccatgcc gtcctctgat aaatgacgcc ggtcgcgaat cacgatcggg ccgacgtcgc
     7861 ctacgccttt gccgtcgatg aacactttgc ctacctgaac gggagtggtc ttgtgggcgc
     7921 tctgggcagt taactctaag acatccccgt cttcgagaat aaagcagtta ttttccggaa
     7981 ccccaacctc ttgagccaag cggcgatggc gaactagatg gcgatactcg ccgtgaatcg
     8041 gcacgaaaaa ccgcggacgc gtcagttgca gcattgtttt caattcttct tggctggcat
     8101 gtccggagac gtgaatttcc gaaaccttct cgtagtgaac ttctgcgccg cggcgataga
     8161 gatgattaat caagtttgaa atggttttct cgttgcctgg gataaatttg gaagacagaa
     8221 tgacgttgtc gcccggttga attttgatgg tcttgtggtc gttgagagct acccggtgca
     8281 aaactgataa tggctcgccc tggctgccgg ttgtcagaaa ggtgacacga tcgggggcaa
     8341 gatcttgcca acggtcattt tccgtcatga aactttgtgg aagtcgcaga tagccaaggt
     8401 cggaagcgac ctggctgttg cgaaccatgc tgcggccgct cagcacaact ctgcgaccac
     8461 aacgttcgga gatttctagc acttgctgga tcctcggaat gctcgaagca aacgtcgaaa
     8521 ccagcacccg gccgttggat tgctgaaaaa tgtgttccag gttcgttcca acttcgcgtt
     8581 cagacccggt gtggccgcgc cgttcaacgt tggtggaatc ggataaaagc agaagcacat
     8641 ctttttcacc ataggcggcg aatctttgaa aatcgaacat ctcgccttcc atcggcgtgt
     8701 tatcgatctt gaagtctccg gtgtgaataa ccgtcccgac gggggtctga atcgccaatg
     8761 ctagacaatc catcaggcta tgagtgacac gaataccttc gagcttgaaa ggacctactt
     8821 cccacagctt gccagcggtg atctcgcgga cgtcggcgac atcttcgaga ccatgttctc
     8881 tcactcgatt gctcagcaga cccagtgtca agcgcgtggc gtagatcggc accgagaggc
     8941 gctcaagcac atagggaatt gcccccatat ggtcctcatg cgcatgcgtc aaaacgatcg
     9001 ctttaagctt gtgtttattt tccaaaagat agctgatatc ggggatgacg agatcgatgc
     9061 cgagcaagtc ggcgccggga aacatcaagc cgcagtcgat ggctatggct gcttcaccgt
     9121 attcgactag catcatattc aggccaaact caccgagacc gcctagcggt atgatcctga
     9181 cggattccat gctgcttaaa tactccgttt ctccaagtga cgatcgctga taatgccgtg
     9241 gcgtgagtta gctgtgacgg aatccaaatc aacgggcgtg cttcgcagat tcttttcgat
     9301 agcttgtcgt acctgatttg acagatggcg aagcgtgccc gggccaaaac catccacggc
     9361 aataggttca ccgataatta cgtcaacggt tcctccccgc aagcgccatg cgccagccgg
     9421 cagaacctgc cggctgccat tgatggtcac ggggacaatc ggtcgatttg attgggcggc
     9481 aagtaaaaat ccgcctttct tgaaacgcag cagttttccg tctcggctgc gcgtcccttc
     9541 ggggaaaact acaatcgaaa taccggccgc catccgttct tttgctcgcc tcaagctttt
     9601 gatagccgcg agaggatccc ctcgatcgat ggtgatatgc cgagatgccc acatcgccca
     9661 gccgaagaac ggaatccaaa gaagctctct ttttgcgatc cagcgcaact gaaaatttac
     9721 caggctctgc accaatactg gaatatcgag attgctctga tgattgacca tgaaaacata
     9781 aggttgtcgt gaatcgatat attcgcggcc gctaactcgc aacgcaatcc cgcccatccg
     9841 caagatcaaa gcggtccaga tctggctggc gcgataggcg ctttttccgt gacgatcaaa
     9901 caggccaaga acaattgtca ccagcgcagc cggcaccgtg gcgatcataa aaacgcacag
     9961 cttgagcagg taaaacatca agatcaaagt aagcttggcg agcgcaaaca acgaaacgaa
    10021 ttacgtaagt ctagtgtacg gatgaacaaa atgcaattga gcttgcgcgc ccgatcggtg
    10081 ccaaatcgat tgttgactat gaataaagcg ggcgctaagt tggctgcact gttgccgccg
    10141 cgaggagcgg ctgggaggta ttcaatgtcc gttaacaaag tcattctcat cggtaatctg
    10201 ggtaaggatc cggagcttcg ctttacctcg accggcagcg cggtagcgcg gtttcccatc
    10261 gcgacttcgg aagtttggaa cgaccgggac ggcaataagc aggaaagaac cgaatggcat
    10321 aacatcatcg tctggggcaa gcagggcgaa tcctgcagcc agtatcttgc caagggaagg
    10381 caagtttttg tcgagggcag cattcgcagt cgaagttatg acgacaagag cggcaccaag
    10441 cgatatgtca ccgaaatcgt cgctcagcga attagatttc tcggcggcgg cggtggcact
    10501 cggatggcac cggagataga agctggcggc gccaatgacg aaatatcgac tgcgggtgga
    10561 cagccgcctt tcgatgacga catacccttt tgagactctt ttctacgcaa cccgattcct
    10621 atctctacca gctaaaaatg ctgcaatatt ctcggccact tcgtcgagca gccgttctcg
    10681 cgcttgacgg gcgctccacg ccgtgtgcgg cgtcaccaac aaattgtcca gcgcgctggc
    10741 ggcgaggatg atcggatgat ccgaggccgg aggttcctca ctgatcacat ccaatgcggc
    10801 ggcggctaag cgctgctcgc gcagtgcctg aatcaatgcc gattcatcga tcaaagcgcc
    10861 gcgcgcggtg ttgatcaaca atgccgaggg tttcatcagt ttaagcatct caccatcgat
    10921 taggtttttg gtctgcggcg tgagcgcgca atggagcgag acaatatcag ctcgcggaag
    10981 gagttgctcc agcggaactc ggtcgggctc atgcggttcc gctgctcctg gacgagcggc
    11041 taccacaact tccataccga agcttcgagc catgcggcca acctcgcggc cgatattgcc
    11101 gtagccaatg atcgctaacg tcttaccgtt aagttcgaca attggatatg tgagcagcga
    11161 gaaaatggga cttttctgcc acatccccgc cttaacatcg ctggcgtagc ggcccgcgtg
    11221 agtagccaat tctaaaatca atgccatcgt gaattgcgct actgactgtg cagcatagcc
    11281 gggtacgttg cagaccttga tgccacgttg ccgcgccgca gtatgatcga taatgtcggt
    11341 gcctgtggca gcgactgcga ttaaggataa gttacgcgac tccggcgcgg cgagagccgc
    11401 ttgatcgatg cttcgtttgt ttgtgaccac gatgctctga ccggccaatc ggccgatcgt
    11461 ttcgccaggc tgggtgactt ggtagattct gcaatggcca atctcgctga agcgtgccag
    11521 tgaaacatcg ccaaatgtgg cggcgtcaag gaagacaatc ttgggcaaag tcatcgtcat
    11581 agccgaccgc gtcgttcgag cgcttttgcc tcctcccata accgatccat ttcttccagg
    11641 ctggattggt ccggcgtttt ccccgctgcc cgcagctgat tttcgagata gtggaatcgt
    11701 tcaatgaacc tgtcgatggt ttgactggcg acatcttctg cctgaacaac cagaaagcgg
    11761 gcaagattaa ccaatgagaa aaacacatcg cccaattcct ctccggctcg ttgacggtcg
    11821 cctgaagccc aggcgttctt gagttcgctc aattcctcct ccactttttg ccacaccggt
    11881 tctatgttgg gccaatcgaa accaactcgc gcggcgcgct ccgaaatcgt ttgcgcacgt
    11941 gccaacgccg gcatggactt tggcaggctc cccaaagctg aattggtatt gccagcgggt
    12001 ttttcttgcc ctttaatttg cgcccactgt ttcgtcacgg cctcggaatc aggtaaggcg
    12061 tcaccgccga aaacgtgagg atgacgccgg acgatttttg ccgctaggtt gctgatcact
    12121 tcaccggcag tgaatgtgcc gttttcggcg ccgatctggc aatgaaagac gatctgatac
    12181 agcaggtctg atagttcctc ggcgatctta gccggatcgt tgccggtaag cgcatcgaga
    12241 agctcatggc tctcttccag caaaaacggc tttaaactcc catgcgcctg ctcgcgaacc
    12301 catgggcagc cattctctcc gcgcattcgg gccatcaact ccaagacttc ggaaaacttt
    12361 tctccagctt ttttgtggtc gaacacaatt tacccgaggc gcgctgcgcg acgaattttc
    12421 tttgcgtgaa gcagcgtgtt aaagcttaca tagctaagca tcatacccgg aggcaatgcc
    12481 gggctgagta ataaaataga gccttaatcg aaggagttac tcaatgtcaa ctgcaaccat
    12541 cgacggactg gaggttcgtt atgaagtgtt cgggtctggt gctccgcttt tgttgttcgc
    12601 gcccggaggg tttgatgcga cgatagagaa gtggcgcgcc gccagcgcct ggaccggcat
    12661 cgatgcccta caaacgctgg caactcggtt tcgagtgatt gcctatgata ggcgcgaagc
    12721 gggtcaatcg ggaggaagag tcgaacgcct cacctggcac acctatgcaa ggcaagccaa
    12781 agctctattg gatcatctca agatcgaatc tgcgcttgtc ctcggcggat gtatgggttg
    12841 ttcggtggca ctagcgttcg cggttgatta ctcacaggcg actcgcgcgc ttttgcttca
    12901 ctggccggtc ggtggttttc gttggaaagc aggcggtctg gaccgctttg aacgccatta
    12961 ccggttcgcc agcacccacg ggcttgaggg agtcgtcgag cgcgcgagaa ccggcaaaag
    13021 tttttggaca gatcccgaag ccggtccctg ggcctcggtg attgctcaag atccctcatt
    13081 cgctgaggac tttgtccgtc aggatttgca tcggtatctc ggactcactg ctgtcagcgg
    13141 cagaaccctg ttcgatcgcg atactgccac cggggcagaa ccggaagagc tcatgggcat
    13201 tagcgcgccc gctcttatcg ttccgggtga cgacgcttct catgccactt ccgcggcgca
    13261 ttacctccgt gagttgttgc ccaatgcgag cttttggaat gtgctaccac cggagcagac
    13321 cacccgtgca gtttgcgatc gcatactgga gttttctcgc caatcctcat gaggcgttag
    13381 gcggctagat tcgagtttga cctcgtgcgg atggaccgat atagaaaatc ttgcagaaga
    13441 cttcgttcat ccaataaatt ttttcaggag gctcgtcatg gacgtgacca catcaactct
    13501 tcaactcagc accgctgatg gcaacatgga cgcgttcgaa gcgcggccga aggatggcgg
    13561 gtcgtatccc ggcatcgttg ttctgatgga agctttcggg cttaacgatc acatcaaaaa
    13621 agtgaccgag cggattgcgc aagaaggcta cgttgcgatt gccccggatc tctatcaccg
    13681 cgaatccgag cgcatcatcc cctatagcga tatgaagaag gcaatcggca ccatgaaccg
    13741 cttgcaggat cccaaggtga tggacgacgt gggagcggcc atcgcgcatt tgaaggggca
    13801 gagcaacgtc aaaggaggcg ccctcggtgt gaccggtttc tgtatgggtg gccgctatac
    13861 ctatcttgcc gcagcgcatc ataacaaaga cgtcaaagcg gcggtgtgct tttatggcgg
    13921 tggcattcca atgggagagc cgagcccgct gtcgcgcacc ggtgagatta cctgtccgat
    13981 ttatctcttt ttcggagcca aagatccgtt gattccaatg gatcaagtcg ggcagatcaa
    14041 caccgagctg acccataaaa aagtgagctt ctcaatgaag gtctatccgg aggcaacgca
    14101 cgggttcttc tgcgatgagc ggcagagcta tcatgagagc gcggctaaag attcgtggga
    14161 gaagttgaaa accttttttg ctcaacactt aaagtaggca gaactaaccg cccatcgccc
    14221 aggcgtggtc tgccgctccg cttaactcat tgatccgggt gggccgttca attgaagcga
    14281 tgcctgtaaa caagaacaaa gatattgtga ctgttggttt ggtgcagatg agcgccagcg
    14341 aagacccgga agccaaccta tcaaaggcgg aagcgcgcat acgagagggg gcgagaaaag
    14401 gcgcgcagat tgtctgcctg caagaactgt ttcgctcgcg ttatttttgc cagagcgaag
    14461 atcagcgatg cttcgccctg gctgaaacta ttccgggacc gagcacggag cgtctgagcg
    14521 ctttagcgcg cgaactgcag atagtcatcg tagcttcgtt attcgagaag cgttcggcgg
    14581 gcatctatca taacaccgcg gtggtgttcg atgccgacgg ctcgattgcc ggaaaatatc
    14641 gcaagatgca cattccggac gatcccctct atttcgaaaa gttttacttc acaccgggtg
    14701 atctgggctt tccaagcttt caaacccgct acgcgcgagt cggcgctctg gtttgttggg
    14761 atcaatggtt tccagaagct gcccgtctga cctcgttatc cggggcggag atactggttt
    14821 atccaacagc cattggctgg ataccgaagg agccgcgcgc cgtagcgcaa acgcagcgta
    14881 acgcctggga gctgattcag cgtgcccacg ctatcgcgaa cggtgtcttt gtggcttctg
    14941 tcaatcgcgt cggcagggaa ggaaatttaa aattttgggg acattctttt gttgctggtc
    15001 catcaggaga aattttgagc gtcgccgccg gcgaccgtga agaaatcctc attgccaaat
    15061 gcgatctgcg caagatcgat gagatccggg agagctggcc cttcttccga gatcgaagaa
    15121 tagacgccta cagttctctg cagtcgcgat ttatcgattc gcccgacggc acctaatcac
    15181 cgctcgtatt caatcactct gatggccgac caagataaac cctcgcagat gccgcgtgac
    15241 ctggggtatt caatgcccgc ggaatggacg ccgcatcggg ccacttggtt atcctggcca
    15301 cacaatcagg aaacctggcc cgctcaactc gagcgcgtta gggaggcttg ggtgcagatg
    15361 attctggcgc tgtcgccgca cgagaaagtt tgtcttctgg tcaatgatga tgccatgcga
    15421 cagcatgtct cgagccgttt gaagacggcc ggtgcggtga tggaaaatgt cacactcatc
    15481 gaaatcccga cggtcgatgt ttggatgcgc gactacgggc cgacatttgt tgtgcgcagc
    15541 cgagcggctg tttcattggc gctcaatgac tggatcttca atggttgggg cggcaaatac
    15601 aaagcttacg aagaggacga tgaggtcgcc aaggctatcg cccagctgct cggcgtgccg
    15661 gtatttgcgc acgcgcttgt ccttgaaggt ggcgccatag aagtcaatgg tgcggggatt
    15721 tgtctgacca ccgagcagtg tctgctcaat aacaatcgta acccgcacct gagccgagcg
    15781 gaaatagagc agtttttgag agaatcgctg ggagtaagtc agatcatctg gctgggcgag
    15841 ggagttgccg gcgatgatac cgacggccac attgatgata ttgcccgctt cgccgacacg
    15901 acgaccattg tctgtgctgt cgaaggcgat ccgcaagatg cgaattatgt tcctctgcgg
    15961 gagaatcttg agcgtctcca gcaagcgcgc gatcagcacg gcagcaggtt cgatgtggtc
    16021 acgttgccct ctccgcgtcc ggtcatttgc caggggtcgc gcttaccggc cagctatgcc
    16081 aatttttata tcgccaatga ggtagttctc gtgccgcttt tcgatgaccc gggcgatcaa
    16141 aacgcgctgg ggactttagg tgagttgttt tcgacccgta aaatcgttgg gattcgctgc
    16201 accgatgtcg ttgcaggtct cggcgccatc cattgcgtga cgcagcaaga gcccaagatc
    16261 gaggggtcga gggcttgaca atttctttct cccgaactta ttcttagctt tgctatgggg
    16321 cgactcattc aaatccgaga cctgcatcag gcgcgacgtc gtcgttccga aaaggtcagc
    16381 atggagcaat gcgttgagct gcttgagtgg aatttgaaaa aaagcctcga tgattatttc
    16441 tcctcgcctc gcgaagagcg ttcgatgaga gcaacgcaaa ttcgaaaact cagtgaaatt
    16501 cttgagtacg cgctgcggct tctctaattc cactcacaat ccgtggaact aaccaagtcc
    16561 ctgccgctgg tcttgctgtt gcttgtcggc ttgacgattc tcggctggtt ttttctctgc
    16621 cgcatgcgct ctctcctccg cgcaacacaa aacgagcagt cctggttatt gatgcagcaa
    16681 cagattgacc agcttcgcgt gcagttcggc caagtgctgg ataacagcac gcagttgatc
    16741 cagcaacagc tgggtcagat gatcggccat gtcaacgagc gtttgcgcga gaattccgaa
    16801 atcctgcagc aaacacacca gagtgttggc caacggctgg acaatgctgc cagagtggta
    16861 ggcgatgtgc agaaaagcct cggcggactc gaagaagcca atcgcaaaat ctacgaagtc
    16921 ggcaaggaca tcgcttcatt gcaggaaatt ctccgctcac ccaagctgcg cgggggacta
    16981 ggcgagttct ttctcgagga cttgttggcg caagtcctgc cgccacagca cttcacaacg
    17041 cagtactgtt ttcgtagcgg cgagaaagtg gacgcggtca tcaggctggg tagctcgctg
    17101 gtgccggtgg actccaagtt cccgctggag aacttcaaaa gaatcatcga taccaaagtc
    17161 gatgacgaac aagcgcgggc gaagaggcag tttgccgcag acgtcagaaa gcatatcgac
    17221 gcgatcgcga caaaatacat attgccagac gaaggaacct acgatttcgc tctgatgtat
    17281 attccggccg agaatgtcta ctatgagatc attattaaag gcgattccaa cgaagagaaa
    17341 agcctaagca cctatgcgat gagcaaacgc gtcgtcccgg tttcgcctaa ctgcttctat
    17401 gcgtacctgc aggcgattgt gctgggactt aagggtatga caatagaaca gcgagcgaaa
    17461 gaaatcattg aatgcttgag ccgtttgcaa ggcgacttca cgcgatttcg cgatgaattt
    17521 ggtctgatcg gcaagcatct gggacatgcc caatccagct atcaagctgc cgagaagcgg
    17581 tttgagcagt ttggacaaaa acttgtgtcg gcgaacgccg ggcagaccga gttatttcaa
    17641 accgcggcgg ccgaccgaaa gcctctctaa ctggctccta cagaagcggc gtctcgacaa
    17701 tgcagttgag cttgttttcc cggttagctc ttggctatct tgccatcttc cttctggtga
    17761 cagcggtgag cacgtatgct gtttttcagc tccaccgggt tgatgagctt accgattccg
    17821 tacttaaggt agataaccga attctcgatt acgaaaagat tcttgccgat ctgctcctgt
    17881 cgcaaagccg ttatgagcag aaatttgcca tcagccgaga tgatgcgctg tatgacgagt
    17941 ttctcagtct aaggcgcgac ttcaacgttc aacttgcaac tgccattacc atcggcgaca
    18001 gcgcggcggg agccatcttg agcaaagttc aacaggatta ccgcaactat gaggagttgg
    18061 tggccaagga agctgcgctt ttgcgcgcga aaaagagcta tcctcaagcg caattcaagc
    18121 aacaaaagga cgcgatcgtc gatgacatac tggtgtcact ggaaaagctg agagccgatc
    18181 aacaatatgc catgtctacc aaggtgaaag agctggcgga agccgcggaa catgcccgtg
    18241 aggtttcggt taccatcacc gtggcatgtc tgatctcgat tgtggtgatg tcgctgatcg
    18301 tgacccggag cattactcga ccgatcgcga ttctgaagac caagacacga gagatcgcgc
    18361 aagggcgttt cgaggggggc ttgaacatca aatcgccgcc cgaactcggc gaattggcgg
    18421 cagcgttcaa tttaatgtgc gagcgattga aagagctcga gcgcatgaag gccgacttct
    18481 tttcatcgat gtcgcacgaa ttgcgcacgc ctctgacttc catcaaagag ggaacggggc
    18541 tgcttctcga cggggtcggc ggcgagacca cggacaaaca acgtaaactg ttggccatcc
    18601 ttgccgagga aagcaatcgg ctcatcgcgg tcgtcaattc cctgctggac ttgtccaaaa
    18661 tggaggccgg gatgatgagc tatgaattcg atctggtgac tatcgagccg ctgattcgcc
    18721 gcgccattgc cgagatcacc cccctcgtcg aagctaaaca aattgaactt aagagcagcg
    18781 tcaatccgtc actgccggcg ctcaggttgg accctgaaag aattttgcaa gtgctgcgca
    18841 atctgctggg taacgcggtt aaatttactc cgaaaggagg ccgggtgagt gtcgttgccg
    18901 cagcggtaaa tggaaacttg caagtttctg tgacagactc tgggcctggt gtaccggccg
    18961 agagcttgac taccattttt gaaaagttta gtcaggggag ccacaaaggc gcaaatacga
    19021 gaaatggcac gggcttagga ttggcaattg ccaagaatat catcaagtcc catggtggtg
    19081 aaatatgggc ggaaagtcag ctagggcggg gaagcacctt tgtgtttgtg ttgcactgtt
    19141 aattctattt ttatccggct gtagcctgct aagagattgg cgcgagcagc gccaggttcg
    19201 tgattcgatg ctccagggcc atagtttgtt tgttaacggc gactacgacg gcgccttcgc
    19261 caaataccgt gaggctttgg ttttagccgg ggatcatacc cccgccgatg ccgccgttta
    19321 caacatcgga ctcatttacg cgcatcctaa caatcctaaa cgcgataacc gcaaagcgat
    19381 ggaatcgttt aaaaaggtga tcagcgagta cgcgcaaagc ccttgggcag agcaatcaaa
    19441 aatatggcta gacgtgctgc agcagttgga agttgcaaag caagaagcgg aaacaacccg
    19501 gcaggccatg gaacaatcta aagaagcgat cgaaagatcg cgagaagacg cggaaaagtt
    19561 tcggttggct gcagagaagt ccaagcagga acttgataga actaggcaag agatggagaa
    19621 atcccggcaa gctctggaga aagccaacca gatcgatatc gaaatcgaac agaagaagcg
    19681 cgaacgaggg aagtagagaa acggacaccc atgtcgttag gcaaaatctt gatcgtcgat
    19741 gatgacgcca acctcctcga gctgataaaa atgcggctcg aatcggcgga ttatgaggtg
    19801 accgcttctt gtgaagagga caatgcgctc gcggctctca aagctgagac gttcgatctt
    19861 tgcatcgttg atttgatgct cgccaaccgt gacggcataa tgttgatgga agagttcaag
    19921 gccgtcaatc ctgaagtgcc gacgatcatc ctcacggcgc acggcagcat ccagaatgcc
    19981 gtggaagcaa tgcgtcgcgg tgcctacagc tatttaacca aaccattcga gcctggcgac
    20041 ctgttgctgc agatcgaaag agctctggag aaccgcaagc ttacctccga gatcaagcgc
    20101 cttaagggtc ttttgcacga gcagttcgat ttcgccaata tcatcgcccg cagcgccaag
    20161 atgcgatcgg ttttggatgt tgtgatgcgc attgcgagac tcgactcgac cgtccatatt
    20221 cacggcgaaa gcggcaccgg aaaggaactc atcgccaaag cgatccacct cgccagcgat
    20281 agaaaagata aaccgtttat cgctttgaac tgcgctgcat taccggaaac actgctggag
    20341 agcgagcttt tcggccacga gaaaggggct ttcaccggtg cggttaaaag caccaggggg
    20401 ctttttaccc aagcgcacgg cggcacactc tttcttgacg agatcggcga catgccgcta
    20461 tcgactcagt cgaaactgtt gcgtgtcctg caggagcggc aattttatcc ggttggaagc
    20521 gagatcccca ccgaagttga tgtgcgagtc atcgccgcca cgaacaaaga cttggaagag
    20581 ctcgtaaaga agggcacatt tagggacgat ctattctatc gaatccatgt gatcccaatt
    20641 tacttaccgc ctctgcgcga gcacaaagaa gacatcgttc cactggtcga tcatttccta
    20701 aagaagtgca ggcagcagat gaagaaggat gtcaaagggt taacgcctga ggcattgagg
    20761 aaattgatgg tgcatgactg gccgggaaac gttcgcgagc tggaaaatac cgtagaatat
    20821 agcgtggcga tgacgcagaa ggagtttgtt acagaggagt atatcctgca aggaaggatg
    20881 tctttctcag acggtgcgca ggcggtcaat gataaaccac cggtctccga tgacgctctg
    20941 agaccgctaa aagatgctcg cgacgcgttc gaaagagatt atttagtgca ggtgctttcg
    21001 atgaccgatg gcaacgtaag tcaagctgcc aagctggccg gcaaatatcg agcggatttt
    21061 tacgacctgc tcaaaaagca tgacctcaaa gcggacgatt ttaaaaaaac caaatctgcc
    21121 gtgagcgcat ctacctgaac cgactccatc attcggcgct cacatcgaag tgagagctcg
    21181 acgctaggtt ggtagcacga acactctcac ctacgtctcc ccgaaacccc tacgcatatg
    21241 actttccttg taggaaatcc cttcgacccg cttctttaaa cgtttcggct ttaggcacaa
    21301 aataaagcg
//
LOCUS       DASUVS010000268         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_108434, whole genome shotgun sequence.
ACCESSION   DASUVS010000268 DASUVS010000000
VERSION     DASUVS010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108434"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2868)
                     /locus_tag="VF182_14705"
     CDS             complement(<1..2868)
                     /locus_tag="VF182_14705"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6438385.1"
                     /translation="MREHGTLHIPDVLAQNDFPMLGSMSNWRTFLALALRHHGELIGI
                     LTARRAEVRPFTPPQIKLLETFADQAVIAIENVRLFNELKGSLEQQTATSEILGVIAS
                     SPTDIQPVLNAVATNAARLCEATDAQIRLVEGDKTRLVASFGNAPALEFSPIIPGRVG
                     GRAILDRKTIHVHDLLEASKIEFPDAGEVATATGTRTILSTPMLREGTPIGVINIRRT
                     DVGPFSEQQIKLLETFAAQAVIAIENVRLFKELQERNVELREALEHQTATAEVLGIIS
                     RSPTDVQPVLDAIVESAARVCGIDDVVLRLRDGDKLLPQAHFGPIPIVRGRMDIDAPE
                     FRWIREHGTLHIPDVRAQSNFPKLGSASGYRSLLAGSLRQQGEFIGLLIARRMEVRPF
                     TPAQIKLLETFADQAVIAIENVRLFNELKESLEQQTATSEILGVIASSPTDIQPVLDV
                     VAESAARLCEASESAIWRCDGANFWLVASQGSIPVPLSEGRRPLTRSIPSARAMIDGE
                     TVHIHDLLSPDSLAEFSEAVRSVGIRTILVTPLLREGVAIGAIHIRRPEVFPFTEKQI
                     ALLQTFADQAVIAIENVRLFKELQDRNAELREALEHQTATSEVLGIISRSPTDVQPVL
                     DAIVESAARVCGVDDVVLRLREGDGLFVRAHFGPVPIADGREQISINELHHRWIREHG
                     TLHIPDVRARRDDFPTVGASAGARSRTFLIVPLGHRGELIGTLNARRIEARPFTPAQI
                     KLLETFADQAVIAIENIRLFQELKESLEQQTATSEILGVIASSPTDVQPVLDTIAESA
                     ARLCSADDSSIRIVEGDILRLMTHKGSIPFFMTGELPISRGSVAGRAVVDDELIHIED
                     LTLRATEFPDVQVALDREGVRTILVAPLERDGRAIGVILIRRIEVRPFSEKQIALLKT
                     FADQAVIAIENVRLFKELQERNAELREALEHQTATSEVLGII"
BASE COUNT          628 a          845 c          804 g          607 t
ORIGIN      
        1 aatgatgccg agcacctccg acgttgccgt ctgatgctcc agcgcctcac gcagttcagc
       61 attgcgctcc tgcaattctt tgaacagccg cacgttttcg atggcaatga cggcctgatc
      121 cgcgaaggtc tttagcaggg cgatttgctt ctcagagaaa ggccgcacct cgatgcgccg
      181 gatcagaata accccgatcg cacgcccgtc tcgctccaac ggagccacca gtatggttcg
      241 aacaccttcg cggtccaagg caacctgcac atcagggaac tcggttgccc taagcgtcaa
      301 gtcctcaatg tggatcagtt catcgtcgac gaccgcccgg cccgcgaccg aaccgcggct
      361 aatcggcagt tctcccgtca taaaaaacgg gatcgatccc ttatgagtca tcagacgaag
      421 gatgtcgcct tccacaattc gaatcgacga atcgtctgca ctgcacagcc gggcggcgct
      481 ctctgcgatg gtgtccagca ccggctgcac atccgtcggc gagctggcga tgacgccaag
      541 aatctcgctc gtcgcggttt gctgctccag cgactccttc agctcttgga acagccggat
      601 gttctcgatg gcaataacgg cttggtcagc gaaagtctct agcagcttga tctgcgccgg
      661 agtgaagggg cgcgcctcaa tgcgacgcgc gttcagggtt ccgatgagtt ctccccgatg
      721 accaagggga acgatcaaaa acgtgcgcga gcgtgcccct gcactcgcgc ctaccgtagg
      781 gaaatcatcc ctacgggcac gcacgtcggg gatgtgaagt gtgccgtgct cgcgtatcca
      841 gcggtggtgc aattcattaa tactgatctg ctcgcgacca tcggctatgg gaacggggcc
      901 aaaatgagcc cgcacaaaca agccgtcccc ttcgcgtaaa cgcagcacca cgtcgtcaac
      961 tccacagacg cgtgccgcac tctcaacaat ggcatcgagg accggttgca cgtcggtcgg
     1021 tgaccggctg atgatgccga gtacttcgga agttgccgtc tgatgctcca gcgcctcacg
     1081 cagttccgcg ttgcggtcct gcaattcctt gaacagccgc acattctcta tggcgatcac
     1141 ggcttggtcg gcgaaggttt gcagaagagc aatttgtttt tcggtaaagg gaaaaacctc
     1201 cggtcggcgg atatgaattg ctccgatcgc aaccccttcg cgaagcaatg gcgtgaccag
     1261 aatagtgcgg atgccgactg accgaaccgc ttcagaaaat tctgctagag agtccgggct
     1321 caaaaggtca tggatatgca cggtttcacc atcgatcatt gcccgggcgc tcggtatcga
     1381 acgagtcagc ggtctcctcc cttcggacag ggggaccgga atagaacctt gcgacgcgac
     1441 aagccaaaag ttggcgccat cacaacgcca gattgccgac tcgctagctt cgcacagccg
     1501 tgcggcgctt tcggcaacga catccagcac cggctgaata tccgtaggcg agctagcgat
     1561 gacgcccaag atttcacttg tcgctgtttg ctgctcaagc gattccttga gttcgttaaa
     1621 cagccggacg ttctcgatgg cgattacagc ttgatcggcg aaagtttcta gcagtttgat
     1681 ctgcgccggg gtaaaggggc gcacttccat acgacgggcg atcagcaatc caatgaattc
     1741 gccttgttga cgaagggaac cggccaaaag gctgcgatag ccgcttgcgg accccaactt
     1801 ggggaaattg ctttgtgcgc gcacatcggg aatgtgcagc gtaccgtgct cacgtatcca
     1861 gcgaaactcc ggggcatcaa tatccattcg gccacgtaca atcggtatcg gaccgaaatg
     1921 agcttgtggc aacaacttgt caccgtcacg gagtcgcagc accacatcat caatgccgca
     1981 aacgcgcgcg gcgctctcga cgatcgcgtc gagcaccggc tgcacgtctg tcggcgagcg
     2041 gctgatgatg ccgagcacct cggctgtcgc ggtctgatgc tccagagcct cacgcagttc
     2101 cacattgcgc tcctgcagtt ctttgaacaa gcgcacgttc tcaatggcaa tgacagcttg
     2161 agcagcgaac gtctcaagga gcttgatctg ctgctcggag aaaggaccga cgtccgtccg
     2221 cctgatgttg atgacaccaa tgggagtccc ctctcggagc atgggcgtac taagaatggt
     2281 ccgtgttcct gtagcagtcg caacttcccc ggcatcggga aactcgattt tcgaggcttc
     2341 caaaagatcg tgaacatgta ttgtttttcg atcaaggatc gctcggccac caactcttcc
     2401 ggggataatc ggtgaaaatt ccagtgctgg cgcatttccg aatgatgcca ctaagcgtgt
     2461 cttgtcacct tcgacgaggc gaatctgggc atcggttgcc tcgcacagcc gagcagcatt
     2521 cgttgccacg gcgttcaaca ctggctgaat atcagttggc gagctagcga tgacgcccag
     2581 gatttcactc gtcgcagtct gctgttccaa agatcctttg agctcgttaa acagccgcac
     2641 gttctcgatc gcaatcactg cctgatcggc gaaggtttcg agcagcttga tctgaggcgg
     2701 ggtgaaggga cgcacctccg cccgacgcgc ggtcagtatc ccaatgagtt cgccatggtg
     2761 gcgtaaggca agggccaaga aggttcgcca gttgctcatg gaacccagca ttggaaaatc
     2821 gttctgcgcc agaacatcag gaatgtgaag cgtgccatgc tcgcgtatcc attggtgatg
     2881 tggt
//
LOCUS       DASUVS010000269         8598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1421109, whole genome shotgun sequence.
ACCESSION   DASUVS010000269 DASUVS010000000
VERSION     DASUVS010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8598
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421109"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..1556)
                     /locus_tag="VF182_14710"
     CDS             complement(288..1556)
                     /locus_tag="VF182_14710"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6438386.1"
                     /translation="MNDQTTSIAAATVAADETSTLEATLRQKRMAIGLISGCHVLNHL
                     QYSITSVIFPVMMTELGFGLVGLGFISALSSFVGQGLQVIYGFLASFFKRTTMLAIGN
                     IVVGVVAMLQYFIGSYPQLVAARVAIDAGSSPQHPLGSSILSRYYPKARGWALTFHHS
                     AGSLGSFIGPAAASLALLYMGWRSAFVIFGSLSLIMGLTVFWVRDHSSARDSAGGKTK
                     TRAGFDAYVQCLRNRNILFTSLVLMAGAAGRGTGVNMTYLVPYFMAQYGVSASGGGLL
                     LTLMQGAGLVGPLAIAWFSDRSGKRRGITQLTLLLSALMTVWLVQHQSLSILFYINLI
                     LYGAFVQARGALTQTMIGDFATPELTDAAFSIYYFVGFISGPIWTLIIGYVMQHYGFT
                     PAFYIAAGTYIAGMALLTFVKEDANTRKPI"
     gene            complement(1716..3260)
                     /locus_tag="VF182_14715"
     CDS             complement(1716..3260)
                     /locus_tag="VF182_14715"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX6438387.1"
                     /translation="MDKPQGFSRSAGSKRNLAVTGVMLVIFLFAIDATIVATSMPTIV
                     AQLGGLELYSWVFSIYMLSSAITTPLFGKLSDLYSRRRLMLIGIGIFVAGSILCGAAQ
                     SMEQLVLFRAVQGLGGGAIYALSFIVVGILFRPEERARMQGIISGIWGIASVLGPLAG
                     GIIVEHWHWRWAFFVNFPITVVAIGLIVVGLKETDQKRRAHKLDIPGMMTLLAALLLL
                     FYALSHSAHSHQPLDTTLAGLIVLALIFLLVFYFIERRAAEPVIPLELFHTSSYRTST
                     AVATLAAMGVFGAITYLPLYLQGVAGLTASRAGMVLLFLSLAWTLGSLVAGQGSNRFG
                     YRAVAASGMILMAVGYSFFVLFGSALTVAWVIVSATLIGTGMGMANLTTLVTVQNSVS
                     PQRIGVATSTLMLFRTFGGAFSVSLMGTVLLHQMQRGLTKLSTSGLSSTLLDKLAHPQ
                     NLLEPVTRAQITPDLLPRLIAILSNAIWYAFLTGFVLMAIGVVASFMLPNARPADAVQ
                     RGHRAG"
     gene            3246..3683
                     /locus_tag="VF182_14720"
     CDS             3246..3683
                     /locus_tag="VF182_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438388.1"
                     /translation="MRFVHVGWFGGFYIEFCFIVIVDLVNEHSQATVAASWPRTYGAH
                     PAAAIHVQLAGVDKNESDWVYVPGVTSILPNSQATRLEKLRREFKNRGTGLRRGRSKA
                     EREITTDFSGSEARTAWGSKRDRHRYLRRTYREKSPPAERHGL"
     gene            complement(3719..4363)
                     /locus_tag="VF182_14725"
     CDS             complement(3719..4363)
                     /locus_tag="VF182_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015141047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6438389.1"
                     /translation="MKILKLSYALALALSFNGSYANAQDLQPGSKAPKGQGITTNGKQ
                     LRSPDVVYVPTPNETVDAMLKVAKVGKDDVVYDLGSGDGRIPIMAVQKYNASRAVGID
                     INPERIKEAQANLKSAGVGERVRFLNEDLFQADISDATVVTLYLLPSLNLKLLPKLLS
                     ELKPGTRIVSHAFDMGSWKPQQTLSAGGSTVYYWTIPAKGTPEYEAASAAASKT"
     gene            complement(4537..4971)
                     /locus_tag="VF182_14730"
     CDS             complement(4537..4971)
                     /locus_tag="VF182_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438390.1"
                     /translation="MAQPSIKFGSNRFICEGDFTIIGLNDSGGVVIGAELPPPTQSVW
                     VEFKDGGAFLNADLSDAEGGLVARIRSNAIEFNKDNFYSLQRLPPNQTPPERIVVTNR
                     QGEAAIDLSLKENVWEFTGDFYAKGQHIVVTGQGLTLNPQSS"
     gene            5330..5800
                     /locus_tag="VF182_14735"
     CDS             5330..5800
                     /locus_tag="VF182_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438391.1"
                     /translation="MADSMRANSPAQLLFGFVAGFLATLIFHQLALALLWIMGMAPSG
                     PFPMAPTKPFGVPAVFSLAFWGGVWGILFALIHRGFPRGGAYWIAAFLFGAILPSLVA
                     LMIVLPLKGRPMGGGWHPPLLLTAFLINGAWGIGTGLILKLFTNWSTGLRKANA"
     gene            complement(5884..7128)
                     /locus_tag="VF182_14740"
     CDS             complement(5884..7128)
                     /locus_tag="VF182_14740"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438392.1"
                     /translation="MVNDIICIDAERMIDGRGGAAVEPARVVIQGERITAAGPADRIK
                     APEGARRISLGARTLLPGLIDAHVHLKGWRSSNPDDVLVTPHPLAALRAAADCRKLLY
                     AGFTTVRDCGGCLGPHLRDAIASREIPGPRILTAYRGLSQTGRVKKVTWALDVTPLRQ
                     EVDGVDDCVRVVREQIGLGADLIKVSITGRVYAPQSDPGQTAYTSEEIKAIVSEAHRM
                     GRRVAAHAQATQGIKNGILGGVDSIEHGIYLDEEACQWMRERNTVLVPTLAYFYRIAT
                     LGEGLGSPAYAVRKAKEVVEAHMKSFALAMRMEITIGMGTDFEGSALFPHGENGVEFD
                     LMVEGGMAERDVIVAATATNAAILGIEQKVGIIETGKLADLIAVPENPLKQISALRHV
                     EFVMQEGRIVRSVIEGISENTL"
     gene            complement(7183..8193)
                     /locus_tag="VF182_14745"
     CDS             complement(7183..8193)
                     /locus_tag="VF182_14745"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438393.1"
                     /translation="MEKTERRSASARWAEIAALIALIVMLQTLARPAGAAERLRVAHS
                     TLTATNSVLWVARDRGIFPRHGLDVSLIYVAGVRSMQALLAQEVEIGSVSGTTAIQAN
                     LSGADSVIIGGISNSVLMSLVATPDITAVEALRGKRVGVTRFGSLSDFMARSYLARAG
                     VTPDRDVAILQTGGYPESVAAMQAGGIQAAMLSPPYHTIAIRRLGFRELIDLSRTTKY
                     QSNALVTLRRFAQSKPAVLTVFMEAYMEAVRVFKQEPEFTIGVLGRAMRSNDRQILEE
                     TYGFYRDYFADIPQPTLEGIQLILDELAPRTPKAREARPQDFVDLRFVQALDAARGKK
                     SP"
BASE COUNT         1904 a         2377 c         2439 g         1878 t
ORIGIN      
        1 tgaaatcgaa gcgatatata aggaattaat ggaccagccg ccggccgtcg tggatttggt
       61 aaaaaagctc gaggagaatt aagcgcgaaa ttcggggacc gggaactttg caaaaacgaa
      121 agttgcccgc cccccccatc atgctttctt ctgcattgtt ggagttgagt cataaattcg
      181 catatgcgat gaaactccaa gaacttcacc gcatacttgg ttttacgggc ggcgataaag
      241 gctttctcgc agtggcgggc tgttagcggt cgataagaaa actgttttca aatcggcttg
      301 cgagtgttgg catcttcttt gacgaacgtt agaagcgcca tgccggcgat ataggtcccg
      361 gctgcgatat aaaatgccgg ggtaaaaccg taatgttgca tgacgtagcc gatgatcagg
      421 gtccagatcg gcccggagat gaaaccgacg aaataataaa tcgagaacgc cgcatcggtg
      481 agctccgggg tggcgaaatc gccgatcatg gtttgcgtca gcgcgccgcg ggcttggaca
      541 aacgcgccat aaagaatcag gttgatatag aacaaaatac ttaacgactg gtgttgaacc
      601 agccatacgg tcatgagggc ggataacagc agtgtcagct gggtgatgcc gcggcgtttg
      661 ccgctgcgat ccgaaaacca ggcgattgcc agggggccca ctaatcccgc gccttgcatc
      721 aaggtcaaga gcagcccgcc gccggacgcg ctgacgccgt attgggccat aaagtagggc
      781 accaggtaag tcatattcac tccggtgccc cgtcccgcag cgccggccat gagcaccagc
      841 gatgtgaata gaatattgcg attcctaaga cactgaacgt aggcgtcgaa ccctgctctg
      901 gtttttgtct tgccgccagc gctgtcacgg gcgctgctgt gatcgcggac ccagaaaacc
      961 gtcaatccca tgattaagct cagcgatccg aagatcacaa acgcgctgcg ccagcccatg
     1021 taaagaagcg cgagtgaggc cgccgccggg ccgatgaacg atccgagact gccggcagag
     1081 tgatggaagg tgagcgccca gccgcgcgct ttcggatagt agcgtgacag aatgctcgag
     1141 ccgagcggat gctgtgggct ggagccggca tcgatggcga cgcgcgccgc aaccagctgg
     1201 ggatagctgc cgataaagta ttgcaacatg gccacgactc cgacgacgat gttaccgatc
     1261 gcgagcatgg tcgtgcgttt aaaaaaactg gcgaggaaac cgtaaatgac ctgaagccct
     1321 tggccgacaa agctcgataa cgccgagata aagcccagtc caaccagacc gaaccccagc
     1381 tcggtcatca tcaccggaaa gatcaccgat gtgatgctgt attgaaggtg attcaaaaca
     1441 tggcaaccgc tgatcaggcc aatcgccatg cgcttttggc gcagtgtagc ttccagagtt
     1501 gatgtttcgt ccgcggcgac ggttgcggcg gcaatcgagg tagtttgatc attcatgaga
     1561 gtgagtataa agcatttgcc agcatcgagt attgagatca ggtgtataag ttgatgcgct
     1621 ccgcgtcaaa cgcggtcgtg atacgactcc ggatgggaaa ttattgccga agtgtgcgcc
     1681 ctggccggcc gtcgattccc gctagtggat tggatttagc cggctctatg gccccgttga
     1741 accgcgtcag cgggtctggc gttcggcaac atgaaactcg cgaccacgcc gatggccatg
     1801 agcacgaacc cagtcagaaa cgcgtaccaa atggcattgc tgagaatggc gataagtctc
     1861 ggcagtaggt ccggcgtgat ctgcgcgcgc gtcaccggtt ccaggaggtt ttgcggatgg
     1921 gcgagtttat cgagcaatgt gctcgaaagc ccgctcgtgg aaagcttggt caagccgcgt
     1981 tgcatctggt gcagcagcac ggttcccatc aagcttacgg aaaatgcgcc gccaaaagtt
     2041 cggaacagca tcaaggtcga cgtcgccacg ccgatgcgct gcggtgagac ggaattctgc
     2101 acggtcacta gagtcgtcag attggccatg cccatgccgg tgccgatcaa tgtagcgctg
     2161 acgatcaccc aggcgacggt caatgccgag ccaaagagaa cgaagaagct gtagccgaca
     2221 gccataagaa tcatgccgct ggccgcaacg gcgcgatagc cgaagcgatt gctcccttgc
     2281 ccggcgacca agctgcccaa cgtccacgcc aggcttagaa acaacaacac cattccggcc
     2341 cgggaagcag tgagaccggc gacaccttga aggtagagcg gcagataagt gatcgcgccg
     2401 aacaccccca tcgctgcaag cgtcgccaca gccgtcgagg ttcgataaga actcgtgtga
     2461 aacaactcca gcgggatgac aggttcggcg gcgcggcgtt cgatgaagta gaaaaccagg
     2521 agaaaaatca gtgccagaac aataaggccg gccagtgtgg tgtcgagggg ctggtgtgaa
     2581 tgtgcgctat gggacagcgc gtagaacaac agcaacaacg ccgcaagcaa agtcatcatg
     2641 ccggggatgt cgagtttatg cgcgcgccgt ttttgatccg tctctttgag cccgacgacg
     2701 atcaggccga tggcgacgac agtaatcggg aaattgacga aaaatgccca acgccaatgc
     2761 caatgttcga cgatgatgcc gcccgccagt ggtccgagca ccgatgcgat gccccagatg
     2821 ccgctgatga taccctgcat tcgggcgcgc tcctccggcc gaaacaaaat gcccacaacg
     2881 atgaacgaaa gcgcgtagat cgcgccgccg ccgagcccct gcacggcgcg gaataacacc
     2941 agctgctcca tgctttgcgc cgcgccacac aagattgagc cggcgacgaa aatgccgata
     3001 ccaataagca taagcctgcg ccggctgtag agatccgaga gcttgccgaa cagcggcgtc
     3061 gtgattgcag aactgagcat gtatatcgag aatacccagc tataaagttc cagtccgccg
     3121 agttgggcga cgatggtcgg catcgatgtc gcgacaatgg tcgcatcgat ggcaaacaaa
     3181 aaaatgacca gcatgacgcc ggtcaccgcc agattgcgct tcgatccggc gcttcgagag
     3241 aatccttgag gtttgtccac gtgggttggt tcggtgggtt ttacattgag ttctgcttca
     3301 ttgtcatcgt tgacctagtc aatgagcata gtcaagcaac ggttgctgcc agttggccgc
     3361 gcacgtatgg ggcacacccg gctgcggcaa ttcacgtcca actagccgga gtcgacaaaa
     3421 acgagagcga ctgggtttat gtccccggcg tcacgtcgat tcttccaaac tcacaagcga
     3481 ctcgtttaga aaaattacgg cgtgaattca aaaaccgtgg gacgggattg cgaagaggtc
     3541 gcagtaaagc cgagcgtgag atcactaccg atttttctgg cagcgaagct aggacggcgt
     3601 ggggttcgaa gcgagaccgg catcgctatc tcaggcgaac ttaccgcgag aaatcgccgc
     3661 cagccgaaag acatggtctt taaaccgtgt ctctcagccg tggcgcagca ctggttgatt
     3721 aagttttgct tgccgcggcg gaggccgctt cgtattcggg tgttcccttg gccggtatcg
     3781 tccagtaata gaccgtgctg ccgccggcgc tcaatgtctg ctggggtttc cagctgccca
     3841 tgtcgaacgc atgcgagaca atccgagtcc cgggctttag ctcagacaaa agtttcggca
     3901 gcaatttcaa attgaggctg ggcagcaggt agagtgtgac caccgttgcg tcgctaatat
     3961 ccgcttgaaa caaatcctcg ttaagaaaac gcacgcgctc gccaaccccg gcagacttga
     4021 ggtttgcctg agcttccttg atgcgctcgg gattgatgtc gatgccaacg gcgcgcgacg
     4081 cattgtactt ctgtaccgcc atgattggga tgcgcccatc gcccgaaccc aagtcataca
     4141 cgacgtcgtc cttgccgacc ttcgcaactt taagcatcgc atcgacggtt tcattgggtg
     4201 tcggaacgta aacgacatcg ggcgagcgaa gttgcttgcc gttggtcgtg atgccttggc
     4261 ccttcggcgc cttggatcca ggctgcagat cctgggcgtt tgcgtaagac ccattgaatg
     4321 aaagcgccag tgcgagcgcg taggacaatt taagtatttt caccaattcc tccttattcc
     4381 tgcatgacgt tgttgagcat tggatctaga ccatgtctca aagctgatga gaatataacc
     4441 ttaaaagaag agtaccgata gattgtgcag cttttttacc tgctgggaaa tcctcggagc
     4501 gatggtttgc tgatccgaga agaagctgat tgaaccctaa gaactctgcg ggttcagagt
     4561 gagtccctgg cctgttacga caatatgttg tcccttggcg tagaagtctc cggtgaactc
     4621 ccaaacattt tctttgagac tcaagtcgat ggctgcttcc ccctgtctgt tggtcacgac
     4681 tatgcgttca gggggcgtct ggttcggagg cagccgctgc agcgagtaga aattgtcctt
     4741 gttgaattcg attgcgttac tccgaatcct ggcgaccaaa cctccctcgg catcagacag
     4801 gtcagcattc aagaatgcgc cgccgtcttt aaattcgacc cacaccgatt gcgttggggg
     4861 aggtaattcg gcgccaatca cgacacctcc tgaatcattg agcccgatga tggtgaaatc
     4921 accttcgcat ataaaccgat tgctgccaaa ctttatgctg ggttgcgcca agtaagtatc
     4981 ctccgcaggg atctataaaa agaatccaca aaccgttttg gaattttccg tcgtacgggt
     5041 cacgccactt caaagctaaa tccggcttaa ctaaactgat caagcaatct tcatagggat
     5101 ttaggcctat gttatcttgc ctgaatagtt gcgggctttg tcatttggcg atccttatag
     5161 gataagcttt cgcgaatgtg tctgaaaatc aagcctctca tagcaagaat ttcgtttggg
     5221 ccccggacta tgggatggtg agcggctggg aggtgcttgc ctcctgggcc gatcgcgcta
     5281 gagataacgt ataggtaggt cgccaagaat ttttctggga ggacgcttta tggctgatag
     5341 catgcgcgcg aactctccgg cacaactact gtttggtttt gtcgccggct ttcttgccac
     5401 tctaatcttt caccagctgg cgctagcttt actatggatt atgggtatgg cgccctcggg
     5461 tccgtttcca atggcgccga ctaaaccgtt tggtgtgccg gcggtttttt cattggcatt
     5521 ttggggcggc gtctggggca ttttatttgc gctgatccac cgtggctttc ctcgcggtgg
     5581 cgcttattgg attgcggctt ttcttttcgg cgccatcctg ccttccctgg tcgctcttat
     5641 gatcgtctta ccgctcaagg gacggcccat gggcggcggt tggcatccgc ctctgctgct
     5701 gacggcattt ttgattaatg gggcctgggg tatcggcacc ggactcattc tcaagctttt
     5761 tacgaactgg tccaccggat tgcgcaaggc taatgcctga ttctacaaga tagctttcga
     5821 gttcctcgcc gctccatagt ttgccttacg tatcgggcag accgatgccg tctccgtgcg
     5881 cgtttataaa gtattctccg agatgccttc gataacggag cggacaattc gtccttcctg
     5941 catgacgaat tcaacgtggc gcagcgccga gatctgtttg agagggtttt ccgggacagc
     6001 gatgaggtcg gccaattttc cggtctctat gattcctact ttttgctcga tacccaggat
     6061 cgccgcattg gtcgccgtag ccgcgacaat cacatcgcgc tccgccatac caccttccac
     6121 catcaaatcg aattcgacgc cgttctctcc atgggggaaa agcgccgagc cttcgaagtc
     6181 ggttcccatt ccaatggtga tttccatgcg catcgccaga gcaaatgatt tcatgtgcgc
     6241 ttcgaccacc tctttggcct tacgcaccgc gtatgcgggc gatccgagac cttcacccaa
     6301 ggtggcgatg cgatagaaat aggccagggt agggacgagc acggtattgc gctcgcgcat
     6361 ccattggcag gcttcttcgt ccagataaat cccatgctcg atggaatcga ctccgcccag
     6421 gatgccgttc ttgatgccct gggttgcctg agcgtgagcc gcaacccgcc ggcccattcg
     6481 gtgagcctcg ctaacgatcg ctttgatttc ttccgacgtg tatgcggtct gccctggatc
     6541 gctttgcggc gcatagaccc ggcctgtgat cgataccttg atcaagtcgg cgccgaggcc
     6601 gatctgttcc cttacaaccc gcacgcaatc gtcgactcca tcaacttcct ggcgtagcgg
     6661 cgtgacatcc agagcccatg tgaccttctt cacgcgtccc gtctgtgaga gtccccgata
     6721 tgcggtgaga attctaggcc ccgggatttc tcggctggct atggcatcgc gcaagtgcgg
     6781 ccctaggcaa ccgccgcaat cacgcacggt ggtaaatccc gcatagagca actttcggca
     6841 atcagccgcc gcgcgcaacg ccgccagtgg gtgaggcgtt acaagtacgt cgtcaggatt
     6901 ggaactgcgc catcctttga ggtgaacgtg cgcatcgatg aggccgggaa ggagggtgcg
     6961 cgctcccaaa gagatccggc gggcgccttc cggcgctttg atccggtctg ctggtcctgc
     7021 ggcggtgatc cgctctcctt gaatcacaac tcgcgcgggt tccaccgcgg caccgccgcg
     7081 accgtcgatc atgcgctctg catctataca gatgatgtca ttcaccatcg aactccttat
     7141 gcgttagata ccaatatggg ttgtggtaga gaactcatcg gttcaaggac tcttcttgcc
     7201 acgcgccgcg tcaagcgcct gcacgaagcg caaatcaacg aagtcctggg gccgcgcctc
     7261 acgcgctttc ggcgtccgcg gcgccagttc gtcgaggatg agttgtattc cctcgagcgt
     7321 cggctgaggt atatctgcga agtagtcgcg gtaaaagccg tatgtttctt ccaagatctg
     7381 ccggtcgttg ctccgcatgg cgcggcccag cactccaatc gtgaactccg gttcctgttt
     7441 gaagacgcgg accgcttcca tataggcttc catgaagacg gtgaggacgg ccggctttga
     7501 ctgggcgaac cgccgcagcg tgaccaaagc gttggattgg tattttgtcg tccggctcag
     7561 atcgatgagc tcgcggaagc ccagccgtct gatggcgatt gtatgatatg gtgggctcaa
     7621 catggccgcc tgaattcccc ctgcctgcat cgccgcgacg ctctccgggt acccgcctgt
     7681 ctgaagaatc gcgacgtcgc ggtctggcgt gaccccggcg cgcgccaagt acgagcgcgc
     7741 catgaaatcg gaaagagaac cgaaacgggt gacgcctact cgcttgccgc gcagggcttc
     7801 caccgcggtg atatcaggag tggctaccag cgacatgagc acgctgttgg agatgccgcc
     7861 gatgatgacg ctatccgctc ctgacaaatt cgcctggatg gcggtggttc ccgagaccga
     7921 accgatttcg acttcttgag cgagcagggc ctgcatcgaa cgcacaccgg cgacgtagat
     7981 caagctgaca tcgaggccat gtcgcggaaa aatgccgcga tcgcgcgcga cccagagcac
     8041 cgaattggtt gcggtgagag tcgaatgcgc tacccgaagg cgttctgccg cgccagcggg
     8101 tcgtgccagc gtttgcaaca tcacaatcaa ggcgatcaga gcagctattt ccgcccacct
     8161 ggcgctcgca gaccgtcgtt cggtcttctc cataaatccc gcccccttca tgctcggagc
     8221 tatacaggat ggcaacgaag ccggtcaagt cagcacgggg aatagcctgc gatttttagc
     8281 cgctcacttt cgcgcatcga tcaaagagta cagcgcaaaa ttgcgaggag tcatcgtgtt
     8341 cgatcgtgct cgttcaaagc gcatgccctt agatgtcgat cacgtcgctt cacttcctct
     8401 caatgcatcc gaaataaaga agcgcggcgg tattgccgga ttgttatgga tcgcggcgat
     8461 ctttttgccg gaggaagttc cctagagggg taaagatcag cagcgccagc gcaatgaata
     8521 aagtcaggaa aatcatcagg acatacaaac tgatcggcgc cgctggctcc tgccgctttt
     8581 cgatcagtgc ggatggct
//
LOCUS       DASUVS010000270        31707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1641004, whole genome shotgun sequence.
ACCESSION   DASUVS010000270 DASUVS010000000
VERSION     DASUVS010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 31707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31707
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641004"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VF182_14750"
     CDS             <1..819
                     /locus_tag="VF182_14750"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX6438394.1"
                     /translation="AEYTMLSHEERKELLKTGTQMVDKRVPVILGCSSPSPREVIELA
                     EYAATVGGDFVQVLMPLRPWGGQPTIAELMEFYTQVASASPLPVVCYHNPGPGADPPQ
                     DAFVKISEIDNIRYFKESSRDITKISRLIEQIELAGRGHYFTTMQPLLVTLMLGGSGA
                     TMPPPGTRIGAQIVKAFRANDLESARFWARCYALFPGKWAAYGLPPVMKSAMKHFGVD
                     IGNPSRPYAPVSPRDHAQIGQFLRQVGLIGEAPPNPKALIDAANFLAQEDTFLR"
     gene            889..1077
                     /locus_tag="VF182_14755"
     CDS             889..1077
                     /locus_tag="VF182_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438395.1"
                     /translation="MEWIKHLSDKLVIWFLRKTSSFGKELTGAGRVEQLLWREQRLRN
                     LKGLSMRYQQRHGGKTSK"
     gene            1088..2083
                     /locus_tag="VF182_14760"
     CDS             1088..2083
                     /locus_tag="VF182_14760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX6438396.1"
                     /translation="MRRSTGRKQIVILGGGCGGVVAATQLGRKLGADHDVILIDRRAD
                     HIFMPAFLFLMTGDRQPQEICRSLSHLTKRNVKVLQSEILGIDPMTQEVSLSKEKISY
                     DYLIVSLGLQTRPDLVSGFTEASLHPWEMESALRLRQALADFRGGRVVVGVPLGPYRC
                     PPAPYEAQWMLDGYFIQRGIRDRVQIDYFTRDPEPTGEAHDPVVWMDDESRRRKIDIH
                     YEFVVRSIDPVKKQVNGLYNYSLPYDLLVMVPPHRPAQVLLDCGLAEAETGIRVDYDT
                     LQTKWENVFAIGDCADMPASKAGGVAHQEADVLAHNLAVEITGKGKPLNLWLHTI"
     gene            complement(2555..3187)
                     /locus_tag="VF182_14765"
     CDS             complement(2555..3187)
                     /locus_tag="VF182_14765"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6438397.1"
                     /translation="MVFTQRALALVKQNRRYFILAGVLILAAIIGISLWQISKSRQNE
                     EAARHFDTAITLFRANKHKEAIPEFKKVEEYRWSHYAALAHLYEANSHLATNDLDAAA
                     AAAQRFIASTDQNSVYRQIGLMTLGHIQEEKNQCAEAIQHYAEAERIAGALKESAVLG
                     KARCYTAVGDINSAIAAYQQYVKENPNSPLAARLMVQIAELQTKTQPTVK"
     gene            complement(3300..3827)
                     /locus_tag="VF182_14770"
     CDS             complement(3300..3827)
                     /locus_tag="VF182_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX6438398.1"
                     /translation="MTKRDDAIQMSVGGLTLDPVTKTPIVILKDTDNKLNLPIWIGLL
                     EATAMATEIEGIKMARPMTHDLIKNILGEVGCAVDSVEITDLKENTYFALVYLNISGR
                     QVVIDSRPSDAIALALRTKSPIFVAKAVLEASSILQQNEEGKEPPVENVSNVSQEKWA
                     EILEKMTPDDFKYKM"
     gene            complement(3824..5182)
                     /gene="miaB"
                     /locus_tag="VF182_14775"
     CDS             complement(3824..5182)
                     /gene="miaB"
                     /locus_tag="VF182_14775"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX6438399.1"
                     /translation="MKKLYIQTYGCQMNQYDSERIAQVMRANYTLIEEPADADLILLN
                     TCSVRDKAEQKVYSALGSWREIKRQKPNVIIGVGGCVAQQEGDRLLKRVSHLDLVFGT
                     HNIHKLPEMIERVQSAHLRPVEIAFYRDPSYMEDPDGRTYVQGAKAFVTIMQGCNKVC
                     SFCIVPHVRGREVSRPSEKILAEIKSLAAQGVKEVMLLGQNVNSYGKTSSGEIHFANL
                     LGRINAIKGLSRIRFTTSHPQDLSADLIEAYATLDKLCEHLHLPVQSGSDAVLLRMRR
                     GYTRSEYLDRIDRLRQRCPEVALSTDIIVGFPGETDADFERTLELLGQVEYDEIYSFM
                     YSPRAQTVSAKLYNDDVPQEKKKERLKQVQDVQQEISLRKNKEKIGRIAEVLIDGQSR
                     NKAQVMGRTRTNRIVNMRGRENLVGEMVNARIIAATVNSLVGELMVRKAASEIQVQGE
                     MV"
     gene            complement(5179..6582)
                     /locus_tag="VF182_14780"
     CDS             complement(5179..6582)
                     /locus_tag="VF182_14780"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6438400.1"
                     /translation="MQDRIIEFANVLRRNGVRVSLSENIDAFRALDLLGVEDASRFRA
                     ALRTTLVKRTVDLRPFEELFDFFFLGIGQSLTQADQQLMEQLGLSPQEFQDLLDRIDR
                     FLKQMEGELSDLTRALLSGNKGELERLLRQAVAAEAEGSNQSFRFTPFTKIASKLELD
                     KLQDEIERLKGHLKMLAQDGEDSESVIRYLDQRLKDLYGMLREIIYQEQRKRGIDASD
                     RNRSVTLAEKSFSFYSEDDIQRMNEAVTRLAQRFKNRLSVRRKNAARGRFDLNETLRK
                     NLQFGGVPFQIQLDRRRKTKPQIVILCDISDSVLNASRFMLQFVYSIQDLYSKVRSFV
                     FVSDIGEVTQLFEEHNIHKAVEMALTGNVIDVYSHSNFGRAFELFYKDYFSAVTNKTT
                     VLIIGDGRNNYNRPSEWVLREIQHKAKQLIWLNPENRMTWGVGDSEMPRYARYCKISE
                     ECRNIGQLYNLIEAIEP"
     gene            complement(6597..7556)
                     /locus_tag="VF182_14785"
     CDS             complement(6597..7556)
                     /locus_tag="VF182_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX6438401.1"
                     /translation="MFQSVDHVIAGFGAQNYICNKNLATVVYLGICLQKPILVEGPAG
                     VGKTELGKVLAGSLGLELIRLQCYEGLDEAKALYEWEYAKQLLYTQILKEKIGDVLQE
                     AKTLEQAVDRITSQDGVFFSERFLLPRPLLRAILSDRRSVLLIDEVDKSDAEFEAFLL
                     EVLSDFQISVPEIGTLKAKHIPLVVLTSNSSREMSDALKRRCLHLHIDFPDAQRETEI
                     VNLKVPGIDQKLTESVIQLIQRIRKLDLKKTPSISETLDWVKALTLLNVRSLDDELVK
                     ETLSILVKYEADIRKAQQELQSYLAERRSLAGAKTLDDKDHLH"
     gene            7662..8426
                     /locus_tag="VF182_14790"
     CDS             7662..8426
                     /locus_tag="VF182_14790"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEX6438402.1"
                     /translation="MCTLALYWKVLPGYSMLVAANRDEHYDRPSAPPAIIGTSPTIVA
                     GRDLRGGGTWLGINENGLLVGILNRRQQPDAIAREAYRSRGLLCLDLLRLTQLDEIEQ
                     IFVDMAPEDYQPFTLVCVDRNRACVAYNTDQAIERIWLPDGLHVFSNSFEFGAPSEKV
                     DRVHGRFLKLAAVRDRPISRGDYLDDAAKALGDHALGDHLSDPRDAICVHGDISGTVS
                     SSIILFSNAARQFQMYYCGGAPCRNPFTLYSTLDVR"
     gene            8423..9142
                     /locus_tag="VF182_14795"
     CDS             8423..9142
                     /locus_tag="VF182_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438403.1"
                     /translation="MSESPNAKPAGTVILLRPGQSQGIEVLLTRRPDEMAFLGGMYCF
                     PGGTVQKEDYSAAMLRRCHGLSPSAARRIIGAHFSPQEALGLWVAVIRELFEEVGVLL
                     AITADGTIATMDRDLNRSLAEKHPALLARRLNFSALLETENLQCDTTQLAYFAHWRTP
                     SQFKVRFDTRFFLASVPAEHAALAPSPEVADAVWLSPDRALQRFAQGTLPMIFPTFAS
                     LRTLADFDSLSSLFEEFTPPK"
     gene            complement(9270..10406)
                     /locus_tag="VF182_14800"
     CDS             complement(9270..10406)
                     /locus_tag="VF182_14800"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX6438404.1"
                     /translation="MAPILPGAVVGVLGSGQLGRMFAIAARQMGYRVHTFSPETDTPT
                     GQIADLEITAAYDDLDAIRDFAENVHVLTFEFENVPAVAVEAAAHFAPVRPSGSVLHI
                     AQHRLREKSFLQQNGFPVAPFRPVNTLDDLCSGIEQIGCPAILKTAVSGYDGKGQVTL
                     TGPGDAEAAFAATGSQEKILEAFIDFELEFSIVAARGQDGSFSHWGLIENTHRDHILD
                     LSFAPARVPQATTVQALEIARNLLEMLDVVGVLCIEFFLSRDRRVFINELAPRPHNSG
                     HLTINAALTSQFEQQLRAVCGLPLGATDFLTAAAMANLLGDLWQDGEPNWSAVAACPD
                     VKLHLYGKDEVRPRRKMGHLTALAPNADEAVERVLYARKRLSQR"
     gene            complement(10415..10909)
                     /gene="purE"
                     /locus_tag="VF182_14805"
     CDS             complement(10415..10909)
                     /gene="purE"
                     /locus_tag="VF182_14805"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX6438405.1"
                     /translation="MPEANNPLVSIIMGSQSDWETMQHASSTLKEFAVPHENLIVSAH
                     RTPAWLSEFAGTAETRGIEVVIAGAGGAAHLPGMTAAQTVVPVLGVPVESAALKGMDS
                     LLSIVQMPGGIPVGTLAIGKAGATNAALLAVAILGNSRPELREKLRQFRTKQSDNVRR
                     QKLP"
     gene            complement(11170..11763)
                     /locus_tag="VF182_14810"
     CDS             complement(11170..11763)
                     /locus_tag="VF182_14810"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018693345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX6438406.1"
                     /translation="MKNCSADERCPWVDPTKPDYIAYHDREWGVPVHDDRTIFEFLTL
                     EGAQAGLSWYTVLRKRAAYRRAFANFDPEKVARYGKRQLDRLMQDAGIIRNQQKIAAA
                     VTNAKSFLAVQEEFGSFDSYIWRFVGGKPLVNTLRALKDYRASSRESDALSNDLKQRG
                     FKFVGSTICYAHMQATGMVNDHVLTCSRRQEIIAAYS"
     gene            complement(11810..12286)
                     /locus_tag="VF182_14815"
     CDS             complement(11810..12286)
                     /locus_tag="VF182_14815"
                     /inference="COORDINATES: protein motif:HMM:NF019468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HEX6438407.1"
                     /translation="MSLICLMVFSVTFVIYTSYFLPSKIATHFNMNNEPDGWMTRSAY
                     LLLILTLLISIPIVISAGFGVLSQKFPHLLNVPNGDYWLAPRRLHESLDFLAAHGHRL
                     GRLVIVLMTGLHFVVLIAHRAEPPMLPQSWLMAILLGFVFSLAMWVLALYRRFPKS"
     gene            12575..13156
                     /locus_tag="VF182_14820"
     CDS             12575..13156
                     /locus_tag="VF182_14820"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX6438408.1"
                     /translation="MNPTRWQYFRELRPFLIAALLLFSLGIIAGKLLAGHSTFAGLKI
                     NESLGGFAQMFINLPRPMLALMIFANNAVKTLLVIVLGLAFAIVPFVFIVVNGVAIGV
                     VLHLATESKGLAYSLLAIVPHGLFELPGVLSGAAIGVMLGNKAIRRLFRKSEFKVGSE
                     LSRAVKIFLTIILPLLAVGAITETYLTAAILGK"
     gene            complement(13196..13990)
                     /locus_tag="VF182_14825"
     CDS             complement(13196..13990)
                     /locus_tag="VF182_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX6438409.1"
                     /translation="MVLREDSHGQAVVRLQRQLKKLGFNPGKIDGDFGPATKAALIAF
                     QKSSALLADGIAGPRTLRALKLVSSDALPSALPGATVTVVSKIFPFTPVDNISANLPY
                     VLSALRRERLVDKPLVLMALATIRAEAECFEPICEGKSKYNTSPGGHPYDLYDFRTDL
                     GNSKRGDGDRYKGRGFIQLTGRRNYEVHGRAIGLGDRLVQTPDLANDGDVAGKLLASF
                     LKAKERVIKEALLEDDLKLARRLVNGGVHGLERFCDAFAIGTRLIA"
     gene            complement(14028..14474)
                     /locus_tag="VF182_14830"
     CDS             complement(14028..14474)
                     /locus_tag="VF182_14830"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX6438410.1"
                     /translation="MSRFEIHPQLLHDCHRIGQFRLCHLLLHKNGAVPWLILVPETAS
                     PGDLLDLPESERNTAMEEAAIAARFIKTEFAVSKINFASIGNVVPQLHLHVVGRRADD
                     PCWPAPIWGNLVEAKGYPVRRIAALTATLARDYSLMAFKGNFPKSD"
     gene            complement(14488..15141)
                     /locus_tag="VF182_14835"
     CDS             complement(14488..15141)
                     /locus_tag="VF182_14835"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX6438411.1"
                     /translation="MVSQLNNQDLHGNVPDESPVVLILIDLINDFEFDGADQMFKNTL
                     EIAQPIAKLKKNAKAAGIPVIYVNDNFGKWQSDFRKLVDHCIEDNVRGKPIAQLLRPE
                     ERDYFVLKPKHSAFYSTSLDLLLRYLKANTLILTGIAGNICVLFTASDGYMRDFNLLV
                     PQDCIASETEADNQYALGYMSKILKADTRPSTDIDLKAMARQKLDELRRQGAPAEEM"
     gene            complement(15236..18331)
                     /locus_tag="VF182_14840"
     CDS             complement(15236..18331)
                     /locus_tag="VF182_14840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX6438412.1"
                     /translation="MPSYVPLWCKTNFSFLEGASHPDELVEESYRFGLPALAVTDRDG
                     VYGIVRAHVKARELGFRLIFGSQISIDDGSSIVLLAQNRAGYANLCRLLTAGRLRSEK
                     GESSVSWDEVCEHAPDLLALWGGDSSLLVAEHEPADVIGPVRDAFDDRLYGMIARHRR
                     EEEFGQEVRLRERARRYLFPLIAANEILYHSSARRPLQDVLTAIRHGIPVASCGRRLK
                     PNAEHGLKSPYLFAQLFADDLAAVARTLEVAERCRFSLDEIHYRYPSERMPDGTTSAE
                     WLRQLTFKGARWRYGEEIPGDIVGQLEKELEVIESLDYPGYFLTMWEIVEFCRANGIL
                     CQGRGSAANSAVCYCLGVTAVDPARIGLLFERFISKERAEPPDIDLDIEHNRREEVVR
                     HVYEKYGRDRAAMAANIVRYRSRSALRDVGKALGLSETSLDRAAKFLSPYEHIRPEIL
                     QQTGIDPAVGLHNQLLQLSNEILDCPRHLSIHPGGFLLGHEPVHDIVPIENGSMAGRT
                     VIQWDKNDLEDLGLFKVDLLGLGSLTQLNLCFRLLREHRRQELSMATIPGKDTATFEM
                     ICRSDTVGVFQIESRAQMAMLPRLRPKTFYDLVIQISIVRPGPITGGMVHPYLRRRQG
                     KEPVEFPHESLVPVLEKTLGVPLFQEQVMRLAVVAADYTPGEADQLRRDMAAWHRSGR
                     MERHRERLITRMQAKGIAPEFAERVFEQIRGFGEYGFPESHAASFALIAYATAWLRCH
                     YPAEFTCSLLNAQPMGFYLPATIVEDAKRHGVVVRPIDVQVSDWDCTLESCADSAAGF
                     AVRMGLRYVKGLAERDWERISQIRHARSFDSLEDFVRRTDLNESSLSLLAEAGAFDGL
                     GVDRRTALWDTRRWAQLRGQSLRLPTEEIDPGFQPLNAFEEVGWDYRTSSHSVRRHPL
                     QPFRGMLAAQGLPDARSIAAKRNGAKVDYAGLVICRQRPGTAGGIVFMTLEDETGFVN
                     VVVWENIFQRYSVLAKTVNFLGISGTLQVEDGVVHLVAEKLWQPKVSTQPAKSSSRDF
                     H"
     gene            complement(18538..19437)
                     /gene="nhaR"
                     /locus_tag="VF182_14845"
     CDS             complement(18538..19437)
                     /gene="nhaR"
                     /locus_tag="VF182_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010585904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HEX6438413.1"
                     /translation="MEWLNYHHLFYFWNVARCGSVSRAGEELRLTQATISAQVKSLEL
                     SIGEKLFRKTGRHLTLTDMGKVVFRYAEEIFSLGRELTGALKGRAGGHVARLTVGVAD
                     VLPKLVAYKLIEPALGLKESYRIICREGSNSDLLPSLAVHDLDVVLADAPIDPAVNVK
                     AFNHLLGECGSTLFATEKLAKVYRRGFPRSLDGAPFLLPTHNTTLRRSLDQWFDSEKI
                     RPMIVAEFEDSALLKVFGARGLGLFVAPSIISAEVRREYDVKALGRVGSIRERFYAIS
                     LDRKLKHPAVVAISEAARVRLSD"
     gene            19543..19959
                     /locus_tag="VF182_14850"
     CDS             19543..19959
                     /locus_tag="VF182_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438414.1"
                     /translation="MTAIRSLFSSEDSGRLFEPDTVLGAQFYATYKNSPYKQPELRLM
                     AAVLEDAISCLSIKPRPATARHRKQLEEARQWFAAEDESEWIFSFKNICEALGIDPAY
                     LRRGLIRWAATELGSDLPRIRTPSSEPRSKIRLRAG"
     gene            20364..21896
                     /locus_tag="VF182_14855"
     CDS             20364..21896
                     /locus_tag="VF182_14855"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438415.1"
                     /translation="MSSFFRKLCLLMSLPSIILVASAERPAAQTRVSIGVTETIETLN
                     PYGDSVSLLYGIWSEITGPFCTYNYDKGDFEGRLAERWKVENPNTWVFYLNKSYRFND
                     GSEVTADDVVHSMMNRVIKDPQSKQKASVAGPIIKAEAVDKHTVRFTTDKPSAPLLGF
                     VCDRLIVTSKAMFDKFGRDIADKEHMLGAGPYRLKELIPGQRMVIVKRSDHPEAKKNP
                     NAPDEIVYRVMRETEQRVTALLNGEIQIAQFIPPHLRQRVEKSPNLKIIPTDSVEIMF
                     LAMQPKPPFDKKEVRQAVCHAINRDQIISTLLEGFASRLDGPIGPGQYGYDPNLKPKM
                     SYDPELSKKLLTQAGHPNGVDVELQTPVGRYTLDKQLTEAMIPMLNAAGFRAKLSTPE
                     WPTLWANVQKGTVPFYYMGRGSVQDPSAALHQYFHSKETPRIGYSNPKLDELLDKEQA
                     EFDPKKRRQYLTEAMSLITDEAPACFMWRHKLLWGMNNRIDYKPLPDARIFGVDIKVA
                     KK"
     gene            21954..22832
                     /locus_tag="VF182_14860"
     CDS             21954..22832
                     /locus_tag="VF182_14860"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6438416.1"
                     /translation="MTGEKFVDVDGIRTRYFDKGNGPVVVLFHGGHFGSNDAADCAED
                     WGLNFDGLAKWFHVYAVDKIGQGHTDNPRTDADYTMAAVVQHAHGFLKALGLRNVHPV
                     GHSRGAYLVARLTLEHPELFRSCILADTNTLAPGVSKNEMVMANPPLPRLSRESQRWV
                     LQRYSFAFEHITEEWLEAMMRVAALQKYQEAVAKMETAGLRTIRFLPHLARQKDETLG
                     WIRERGLAKPTLLVWGYNDPTATLDQGQALFDLLARSTPDSRMQVFNRAGHFTYREHP
                     VEFNELLRSFIQRNSG"
     gene            22883..23884
                     /locus_tag="VF182_14865"
     CDS             22883..23884
                     /locus_tag="VF182_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013368602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6438417.1"
                     /translation="MASYLLHRLAGTIPVLLLISLLVFLLIHAAPGDPTLLLLGEETN
                     AAEVAKAKERWGLDRPLHVQYVKFLSSAVAGDFGKSFKYGEPVTSVIKSRLPATIELA
                     VVSIIIATCLAIPLGVWAGSRPNSWLDNFGTTFGLFGISMPSFWLAIMLILLMAGILN
                     LLPTSGRSTYGVAGPEQTGFYILDSLLQQNWQAAWDGLRHILLPALALGVNMLGILMR
                     VTRSAILEVMNEEYVTTARAKGLAERKVVWRHVTSNALIPVITVVGLELGTLLSGSII
                     VETVFAWPGSGSLLITALNARDYPLVTGLVLSYTVAFVLINLIIDGLYAVIDPRIRF"
     gene            23853..24749
                     /locus_tag="VF182_14870"
     CDS             23853..24749
                     /locus_tag="VF182_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010140748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6438418.1"
                     /translation="MRSLTLESASEKNPGVVASALGRLHAKAFSGGLIVLLFIFLGIF
                     GPSLAPYNPNKQELTAMMKPPQGVGSEHVLGTDNLGRDILSRIVYGARVSLLVAFAVV
                     FVSGFIGIGLGAISGYIGGKADFLIQKLVEVVWAFPPLLLGITIMAFLGQGLTNLIIA
                     LVAQRWIPYCRVVRGQALSLRERDFVMAARCLGATKTQIIGRHILPNLIQTSLVIGTF
                     AMASSIIAEASLSFLGVGVPPEIPTWGTMLADARIYISTSWWMPLFPGLCIFITVLGI
                     NLLGDALRDILDPRLKRTGSGV"
     gene            24857..26260
                     /gene="pyk"
                     /locus_tag="VF182_14875"
     CDS             24857..26260
                     /gene="pyk"
                     /locus_tag="VF182_14875"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX6438419.1"
                     /translation="MRRTKIVCTIGPASASRKVLTGLIQAGMNVARLNFSHGDYSFHK
                     RVIQNIRQIARRLDKPVAILQDLAGPKLRLGPVAADRVRLAARRPFVLTTRPILGSEL
                     AVSVNYKGLTRAVKKGDPILLGDGEIELEAVKVGAHEVTCKVIFGGILGSHKGIHFPR
                     SSLDVRSLTARDKQDLAFGIEQKVDLVALSFVRNSEDIVYARREMNNRGASIPIIAKI
                     EKHEALEHIDGILEKVDGIMVARGDLGLEIAPEKIPAMQKMMIRKANALGKPVITATQ
                     MLRSMVQNPRPTRAEVADIANAVLDGTDALMLSEESAAGDYPVESVRTMARVAEETEQ
                     ILEPRYQFAGRRQSVPEAISFAAISLARDLNVKAFLIPTSSGSTARMISRYRPPHPII
                     AISPEPQTVKMLCLVWGVYPVQVPGFQSTDVMLKVAQQKSVKLGFVKRGDLVAITAGL
                     PLHQAGTTNMITVKVVE"
     gene            26524..27534
                     /locus_tag="VF182_14880"
     CDS             26524..27534
                     /locus_tag="VF182_14880"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6438420.1"
                     /translation="MSEALKAFEDELESKNFKGYWQNIQGDVYREPVPSYEPCHWKGK
                     ELFAAIEKAGEVVGLDLSFRRVIQLWNPSLKNGTTRTLVLNLQMLKSGEEALSHRHIA
                     GAVRFVVKGSGARLIVEGESFEIGEGDFATTPSWTWHDHENNAGQTMLWLDGLDSPLV
                     RLLETDFHEPDPRKKQTVTKPEGYSASTLGALRPSWMRAHSLQPPAFAYKWSDTENAL
                     NKIGEQPGDPYDGIVLEYANPLTGGPTLPTMSCQIHMLRSGETTKSHRHTSSTIYYVH
                     RGQGVSTIGDKRYEWEQGDSFVVPLWYHHHHENTGKDPAIFFVMSDKPLMDAIGHYRE
                     QT"
     gene            27595..28338
                     /locus_tag="VF182_14885"
     CDS             27595..28338
                     /locus_tag="VF182_14885"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6438421.1"
                     /translation="MFNLAHRLSPDEFIDTFFEVNKRDHQRPPHPWDTLFRNGECTKE
                     QLQGWAKERYYFTKQVPIKEYSILYNCPFPEVRRSWLPKAIEEEGEDLIGRPGKPHPE
                     YWLDVCEGLGLNREFARNSEPLYGVKFAVDSFANAAFKNSWLMGVAVSEGDDTAKAMI
                     RDLEVFRKHYTWVPDPALEFYKLHAGVDVEHGILRKEILKKYATTKELQEDCINAQLM
                     KNNMRRVMADCIYMAYVVQGMTCESSAAA"
     gene            28460..29431
                     /locus_tag="VF182_14890"
     CDS             28460..29431
                     /locus_tag="VF182_14890"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438422.1"
                     /translation="MIRSRTTILLLLITVVLPNRAGAAPLRIAYSSISGAMLPLWVAK
                     DKGLFTKHGVDVDLTYIRGVSIEALLAGEVQFVRASPPSVVRSALRGSDLAFIANTIN
                     VAVFSLMSRPELQKPEDLKGKKIGVTNLGDSPDLVLTLLLDKWGLQRGKDLAVLGIRG
                     GMPELLISVSKGFVDAGMISAPSNLRGIKMGLREFVDTADLGIPYTNSPLSTRRSTIK
                     NQRDTVLRVLKGYYEGVQETRRDRNGAMKILAKYVRLTDPDILFEVYQTYGAKLLQPD
                     IKVDLDGVKGLLKGLGSEAVGPNPATFVDLSLVEQLEREGFISTGAR"
     gene            complement(29513..30142)
                     /locus_tag="VF182_14895"
     CDS             complement(29513..30142)
                     /locus_tag="VF182_14895"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX6438423.1"
                     /translation="MIRVAKISLMMIAAVAISPTGLALAASSSTKAESSRESKSKLPS
                     GEERFLKEAASGGLMEVELGKFAAEKGSHQRVKEFGKRMQTDHSKANTQLKKIASGKG
                     VDLPTEPSGEHKSTMDKLTKLSGAEFDREYMDAMVDDHKEDIEKFQTQADKGKDPDLK
                     KFASETLPVLKKHLELAQSTEKQIKTESKGDAKSGSRSRAKSGSKESAR"
     gene            complement(30465..31442)
                     /locus_tag="VF182_14900"
     CDS             complement(30465..31442)
                     /locus_tag="VF182_14900"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438424.1"
                     /translation="MLLKFLPWSLSFVMCMGISSGLAVGQETVTVALTSKAFQYTILP
                     IAQDRGYLKEEGIDLKLVYMQNAPGLQALTAGNVQFSGSGSSALVAISKGGAPFKTVI
                     AANDQVLQWVVVRPNISSIADLKGKKVGTTGVASIAAFMFRNIMGKYGMDGNKDVVFV
                     DPGPVNRLPSLLSGAVDAAILSPEERYAAIDQGMKDLMFMGKEVKNSWGTIATSDRFI
                     KEQPKVMAGFARAVVKALRFVRQNREGAIAAATKFTELDNALVIRMYDDIAKTFTANG
                     VVDEEAQRNDLAIVREIAGVTEVVPIQRAYDFSFARQADQQLTKAGWKP"
BASE COUNT         7247 a         8710 c         8363 g         7387 t
ORIGIN      
        1 gccgagtaca ccatgctcag ccatgaagag cgcaaggagc ttttaaaaac cggcacacag
       61 atggtcgaca agcgggtccc cgtcattctt ggctgttcta gcccatcgcc tcgcgaagta
      121 attgaactgg ccgagtatgc agccacggtc ggcggcgatt ttgtgcaggt gctgatgccg
      181 ctccgcccgt ggggcggtca accgacgatc gcggagttga tggagtttta tacccaagtc
      241 gcatcggcaa gtcctttgcc ggtcgtgtgc tatcacaatc cagggccggg cgccgacccg
      301 ccgcaggatg cgttcgtaaa gatcagcgag attgataaca tccggtactt caaggaaagc
      361 tcgagagata tcacgaagat ttcccgttta attgagcaga tcgaacttgc cggccgcggt
      421 cactacttca ccaccatgca gcccctgctt gtcacgttga tgctcggcgg ctccggcgcc
      481 actatgcctc cgccgggaac ccgcatcggc gcgcaaatcg tgaaagcgtt tcgggcaaac
      541 gatctcgaaa gcgctcgttt ttgggcgcgc tgctatgcat tgttcccggg aaaatgggcc
      601 gcttacggtt tgccgcccgt gatgaaatct gccatgaagc acttcggggt cgatatcggc
      661 aacccgtcac ggccgtatgc tccggtaagc ccccgcgatc atgctcaaat tggccagttt
      721 ctccggcagg tgggattgat cggtgaagcc ccaccgaatc caaaagcttt gatagacgcc
      781 gctaatttcc tcgctcagga agataccttt ctccgctagc cggacgatga atacccgata
      841 ggagaatcgt ctcggtcaat atttccgctc ggtttcattg gagttgcgat ggaatggatt
      901 aaacatcttt ccgacaaact ggttatttgg tttctccgta aaacttcgtc tttcggcaaa
      961 gagcttactg gcgcgggccg agtggagcag ctcttatggc gagagcagcg gttgcgaaat
     1021 ttaaaaggtc tctcaatgcg ctaccagcaa cgccacggcg gcaaaacttc aaaataggtt
     1081 ccgatctatg cgtcgatcga ctgggcgaaa acaaatcgtg atcttgggcg gcggctgcgg
     1141 cggcgtggtt gccgccactc agcttggccg gaaattgggc gccgaccatg atgtgattct
     1201 catcgaccgc cgcgcagatc acatttttat gccggcgttt ctattcctca tgacaggtga
     1261 ccgccaaccc caagagattt gtcgcagtct cagccatctg accaagcgca acgtgaaagt
     1321 gcttcaatcg gaaattctag gcatagaccc tatgactcaa gaagtcagtc tttccaagga
     1381 aaagatcagt tacgactacc taatcgtttc tttagggctg cagacgagac cggacttggt
     1441 gagcggcttt actgaggcat cgctccatcc atgggaaatg gaatcggctc tcaggctacg
     1501 gcaggcacta gctgattttc gcggtggaag agtcgttgtc ggcgtgcctt tgggacccta
     1561 tcgctgcccg ccggcgccgt acgaagcaca gtggatgttg gacggctatt tcatccagcg
     1621 aggtattcgc gatcgtgtgc agatagacta ctttacccgg gatcctgagc cgaccggcga
     1681 agcccacgat ccggtggttt ggatggatga tgagagcaga aggcgcaaaa tcgatatcca
     1741 ttacgaattt gtcgtgcgct caatcgatcc agtgaaaaaa caagtgaatg gactgtacaa
     1801 ctatagcctt ccgtacgatc ttttggtgat ggtcccgccg caccgcccag cgcaagttct
     1861 cctggattgt ggtttggctg aagccgaaac tggcatacga gtggattacg atacactgca
     1921 aacgaaatgg gaaaacgtct ttgcgatcgg cgattgcgcc gatatgccgg cttcaaaagc
     1981 gggaggggtc gcccatcagg aagctgacgt gctggcgcac aatttggctg tagagatcac
     2041 tggaaagggc aagccgctga acttgtggct tcacaccata tgactactcg cgttcggcgc
     2101 tggacgcgcc gcggcaaata gaaccatcta tccgaccgga tggccgcctt acggagccac
     2161 gtttccgcga acatgggggc ccaatcgact gatctactgg gctaagattg gttttgagaa
     2221 atggtggttg tggcggtatt tttagagcgc tcggttagtc ccagacatag ccaagatcga
     2281 gcggcataaa tcaattctac atctggcgtt caaactttcg tcccacttta aaccgaagac
     2341 gttctgaatc tccgcttcaa acccacttta tagcccgtgt tcagtagcag ggtaaggtgg
     2401 caaagacggc acctcgaaag gcagagccga agaaagttga cataatatat cttatcagac
     2461 gttctaaacc aatctagttt gttcaccggg ccttttattt tccagcgtac tgcaggaccc
     2521 ctacaaaaac cggttgatct cgacaggcaa tgctttactt aacggttggc tgcgttttgg
     2581 tttggagctc ggcaatttgc accatgagac gtgcggcaag tggtgagtta gggttttctt
     2641 tgacgtactg ctggtatgcg gcaatggccg agtttatgtc accgacggcc gtataacacc
     2701 gcgcctttcc gagcacggcg gattctttca gagctcccgc aattcgttcg gcttcggcat
     2761 aatgttggat tgcctcggcg cattggtttt tttcttcttg tatatgtcca agggtcatca
     2821 aaccgatctg tcggtagacg gagttctgat cggtggaagc gataaaacgc tgcgccgcgg
     2881 cggctgctgc gtccaaatcg tttgttgcga gatggctgtt ggcctcgtaa agatgcgcca
     2941 aggcggcata atgagaccat cgatactctt cgaccttttt aaattccgga atagcttcct
     3001 tgtgtttgtt ggcgcgaaag agcgtgatcg ccgtatcgaa atgccgggct gcctcctcat
     3061 tttggcggct tttagaaatc tgccaaagcg aaatgccgat gatcgcagcc aggatcaaaa
     3121 cgcccgcgag aataaaataa cggcggtttt gtttaaccag agctaaggct cgctgcgtaa
     3181 aaaccacgaa gctatccggt tggcggagtt ctttacgcga aatatgttta acgctagtcg
     3241 ccatggcttg tgatgggcgt gaaatggaga tggattatca aaggcaggtc aaggaaccgc
     3301 tacatcttat atttgaaatc atcgggcgtc atcttctcta agatctctgc ccacttttcc
     3361 tgggacacgt tggacacgtt ttcgaccgga ggctccttcc cttcctcatt ctgttgaaga
     3421 atgctggagg cttcaagaac tgccttcgcg acgaaaatcg ggctctttgt gcgcagagct
     3481 aacgcaatcg cgtcgctggg acgcgagtcg ataaccacct gacggcctga aatgtttagg
     3541 tacaccagcg caaaataagt attttcctta agatccgtaa tttcgacaga gtccaccgcg
     3601 cagccgactt cgccaagaat gttcttgatt agatcgtggg tcatgggccg agccattttg
     3661 attccttcga tttccgtggc catcgctgtg gcctccagca aaccaatcca gatgggcaaa
     3721 ttcagcttat tatcggtgtc ttttaaaatg acaatgggag ttttcgtcac tgggtcaagg
     3781 gtcagcccac ccacggacat ctgtattgcg tcatcccgtt ttgtcatacc atttccccct
     3841 gcacttggat ttctgaagct gcttttcgaa ccatgagctc cccgaccagc gaattaactg
     3901 ttgcagcgat gatccgtgcg tttaccatct cgccgaccag attctcacgc cctctcatat
     3961 ttacaatccg gttggttcgt gtccgtccca tcacttgcgc cttattccgc gactggccgt
     4021 cgatcaatac ttccgcgatt cgtccgatct tttccttgtt ttttcttaga gaaatttcct
     4081 gctgaacatc ttgcacttgc ttcaaccgct cttttttctt ttcttgtggg acgtcgtcgt
     4141 tgtaaagctt tgctgatacg gtctgcgctc gcggcgagta catgaacgag tagatctcat
     4201 catactccac ttgacccagc aattctagcg ttctttcgaa atcggcatcg gtttccccgg
     4261 ggaatccgac aatgatgtcg gtgctcaagg caacctcagg acaacgttgt cgcagacgat
     4321 cgatacgatc gagatactca ctgcgcgtat aaccccggcg cattctcagg agcacggcat
     4381 cggaaccgga ttggacaggc aagtgaaggt gttcgcaaag tttatccaga gtagcgtaag
     4441 cctcgatcag gtcagccgaa agatcttgcg ggtgagaagt cgtaaaccga atccgcgaaa
     4501 gtcctttaat cgcattgatg cgcccaagaa ggttcgcgaa atggatttcc ccggaagatg
     4561 ttttgccgta ggagtttacg ttctgtccaa gcagcatcac ttctttgacg ccttgagcag
     4621 cgagcgattt tatttcagcg agaatttttt cgcttggccg gctaacttcg cgaccgcgaa
     4681 cgtgaggcac gatgcagaag ctgcaaactt tattgcatcc ctgcatgatt gtgacgaacg
     4741 cttttgcgcc ctgcacgtaa gtcctgccgt cgggatcctc catgtacgaa ggatcgcggt
     4801 agaacgcgat ctcgacagga cgtaggtgag ctgactgaac tcgttcgatc atctcgggaa
     4861 gcttgtggat attgtgggtg ccgaacacga gatccaaatg ggaaacacgc ttgagaagcc
     4921 ggtcgccctc ttgctgggcg acgcaaccgc cgacaccgat gataacgttt ggtttctgtc
     4981 gtttgatttc gcgccagctg ccgagcgcgc tgtaaacctt ttgctccgcc ttatcgcgca
     5041 cgctgcaagt atttaataga atcagatccg catctgcggg ttcctcgatg agcgtataat
     5101 ttgcccgcat gacctgcgcg atccgttccg aatcgtattg attcatttgg cagccgtagg
     5161 tctgaatata caacttcttc atggttcgat ggcttcgatc aggttgtaga gctggccgat
     5221 gttgcggcat tcctcgctga tcttacagta gcgcgcatag cgcggcattt cactatctcc
     5281 tactccccag gtcattctat tttcaggatt gagccagatc agttgtttgg ccttgtgctg
     5341 gatttcccgt aaaacccatt cgctgggacg attgtagttg ttacggccgt cgccgatgat
     5401 caggaccgtc gtcttattgg tgacggcgga aaaataatct ttgtagaaca gctcaaatgc
     5461 ccggccaaag tttgagtgcg agtaaacatc gatgacattg ccggttagcg ccatttcaac
     5521 ggccttgtgg atattgtgtt cttcgaagag ctgagtcacc tcgccaatat cgctgacgaa
     5581 gacgaaactt cgcacctttg aatacagatc ctgaatagaa tagacgaatt gcagcataaa
     5641 gcgcgatgca ttcaaaacag aatcggaaat atcgcacaag atcacgatct gaggtttggt
     5701 ttttcttcgc cggtcaagtt ggatttggaa cggaacgccg ccaaattgga gatttttgcg
     5761 caatgtttcg ttcaagtcga agcgcccgcg agcagcattc ttgcgcctga cagaaagcct
     5821 gttcttgaac cgctgcgcca agcgtgtcac cgcttcgttc atccgttgaa tgtcgtcttc
     5881 gctatagaaa ctgaagctct tctcggccaa tgtcacggat cgatttcgat ccgaagcatc
     5941 gattccacgc tttcgctgtt cctgataaat gatctcacgg agcattccgt aaagatcttt
     6001 gagacgctgg tcaagatagc gaatgaccga ttctgagtct tcgccgtctt gagccagcat
     6061 tttcagatgc cctttgagcc gttcgatttc atcctgaagc ttgtcgagct caagtttgga
     6121 cgcgattttg gtaaaaggtg tgaaacgaaa actttgattg gagccttcgg cttcggcggc
     6181 aactgcttgc cgcagcaacc gttccaactc tcccttgttg ccgctgagca gagcccgggt
     6241 gagatcggac agctcgcctt ccatttgctt gaggaacctg tcgatacgat caagcaggtc
     6301 ctgaaactcc tggggtgaga gaccaagctg ctccatcaat tgttggtccg cttgagtgag
     6361 agattggccg atacccaaaa agaaaaaatc gaaaagttct tcgaaaggcc gcagatcaac
     6421 cgtgcgtttt accagcgtcg ttcgaagggc ggcgcgaaac cgtgacgcat cttcgacgcc
     6481 caataaatcg agggcgcgaa atgcgtcgat attctcggac aaagatacgc gcacgccatt
     6541 gcgcctgaga acgttggcga attcgatgat ccggtcctgc attcaatccc tatcgatcaa
     6601 tgcagatgat ccttgtcatc tagggtcttg gcccctgcca gactccggcg ctccgccaag
     6661 tagctttgta gttcttgctg cgcctttcgt atgtcggctt catatttcac gagaatcgat
     6721 aaagtttcct tcaccagttc atcatcgagc gatctaacgt tcagcaaagt taaagctttc
     6781 acccaatcga gtgtttcgct gatactcggc gtttttttca ggtcgagttt acgaatgcgc
     6841 tggatcagct gaatgacgct ctcggtcaat ttctggtcaa ttcccggaac cttgaggttg
     6901 acgatttcgg tttcacgttg ggcgtcgggg aaatcgatat gaaggtgcag acaacggcgt
     6961 ttcagggcgt cggacatctc acggctgctg ttgctggtaa gcacgaccag cggaatatgt
     7021 ttagccttca aagtgccgat ctcggggaca ctgatctgaa aatcactcaa aacctcgagc
     7081 aggaaggctt caaattcagc gtctgattta tccacttcat cgatcagtaa aacgcttcgc
     7141 ctgtcggaca aaatcgcgcg cagcaatgga cgcggtagca aaaaacgttc ggaaaagaag
     7201 acaccgtcct ggctggtaat gcggtccacc gcctgttcca gagttttagc ttcctgtaag
     7261 acgtcgccga tcttctcctt gagaatctgc gtgtagagca actgttttgc gtactcccat
     7321 tcgtaaagcg ccttggcctc gtccaagcct tcgtagcatt gcaatcggat cagctccaac
     7381 cccaatgaac ccgccaatac tttaccaagc tcggtcttac cgacaccggc tggaccttcg
     7441 accagaatcg gcttctgcag gcaaataccg agataaacga cggtggctaa attcttgtta
     7501 cagatgtagt tttgagcgcc aaaaccagcg ataacgtgat ccacggattg aaacatgaaa
     7561 acctctcaat tctctgaacg ctaacacaat tgcgctatga caacaaatct acccgattga
     7621 agtttcatgt tagattcctt acgcttctgt ggattatcat catgtgcact ttagcgctct
     7681 actggaaagt tcttccggga tattcgatgc tcgttgcggc caaccgcgac gagcactatg
     7741 accggccgtc cgcgccgccg gcgatcattg gcacgtcgcc aaccatagtc gccggccggg
     7801 acttgcgcgg cggtggcacc tggttgggaa tcaatgaaaa cggtttgctg gtgggcattc
     7861 tcaatcggcg gcaacagccg gatgcgattg cgcgagaggc ataccgatcg cgcggtctgt
     7921 tgtgtctgga cctcctccgt ctaacgcaac tcgatgaaat cgagcagata ttcgtcgata
     7981 tggcgccgga ggattatcag ccattcacac tggtctgtgt cgataggaat cgcgcttgcg
     8041 tcgcttacaa taccgatcaa gctattgaac ggatctggct tcccgatggg cttcatgttt
     8101 tcagcaatag cttcgaattc ggcgccccct ctgaaaaggt tgatcgcgtc cacggacgtt
     8161 ttctcaagct cgctgcagtc agagatcggc cgatcagccg cggagattac ctcgatgacg
     8221 cagctaaagc gctcggcgat catgcgcttg gcgatcactt gagcgatcct agggatgcca
     8281 tttgtgttca cggcgatatc tcgggaaccg tttcttccag catcatcctt ttttcgaatg
     8341 ccgcgcggca gttccaaatg tactactgcg gcggcgcccc ttgtcgaaat cctttcactc
     8401 tatattcgac gttggacgtg cgatgagcga atcgcctaac gcaaagcccg ccggcacggt
     8461 cattttattg cggccgggcc agtctcaagg aatcgaagtg ctgctgacgc gccgacctga
     8521 cgagatggcc ttcctgggtg gcatgtactg ctttccaggt ggcacggtgc agaaggagga
     8581 ttactctgcg gcgatgctcc gccggtgtca cggtctctcc cccagtgccg cgcgaaggat
     8641 tatcggcgct cacttcagtc cacaagaagc gctggggcta tgggtggcgg tgatccgaga
     8701 actcttcgaa gaagtcggag ttctgctggc catcaccgcc gatggaacca ttgcgacgat
     8761 ggatcgggac ttgaaccgtt cgctggcgga aaaacacccc gcgctgctgg ctcgacgctt
     8821 gaatttcagc gcgttgctcg agaccgagaa cttgcaatgt gacacaaccc aattagctta
     8881 tttcgcacac tggcggacgc cgtcacagtt caaggttcgt ttcgacacgc gttttttcct
     8941 ggcttccgtt ccggcggaac atgcagctct agcgccttct cctgaagttg ccgacgcggt
     9001 ttggcttagc cccgatcgcg ctctgcagcg attcgcccaa ggcacgttgc cgatgatctt
     9061 tccgacgttt gcatcgttaa ggactctagc ggatttcgat tccttgagca gcttgtttga
     9121 agaattcaca ccgcccaagt gattagcccg gctagcaagc agccgctagc aataacgtaa
     9181 gaggctaaat gaaatgattg cagcaggaac gatcgttata actatgtcac cggctgagaa
     9241 agcctcgcga tcttgcggtg cgacacacgt catctctggc tgagccgttt acgagcgtat
     9301 agcacgcgct cgaccgcttc atctgcattc ggggctaacg cggtcaaatg gcccatcttt
     9361 ctgcgcggac ggacttcgtc tttgccgtat aaatgcaatt tgacgtccgg gcaagccgcc
     9421 actgcagacc aattaggctc accatcttgc cagagatcac ctaacagatt cgccatcgcc
     9481 gctgctgtga gaaaatcggt ggctcctaaa ggcaacccgc atactgcgcg cagctgctgc
     9541 tcaaactgac tggttagggc ggcgttgatg gttagatgtc ccgagttgtg agggcgcggg
     9601 gcaagctcgt tgataaaaac tcttcggtcg cgactaagaa agaattcgat gcacaggacg
     9661 ccgacgacat ccagcatctc tagcaggttt ctagcaatct ccagcgcctg aacagtggta
     9721 gcttgaggca cccgtgccgg tgcaaatgac aagtccaaaa tatgatcgcg atgtgtgttc
     9781 tcgatcaggc cccagtgtga aaaactgccg tcctggccgc gggcggcgac aattgaaaac
     9841 tccagttcga aatcgatgaa ggcttcgagg atcttttcct gactgccggt cgccgcgaaa
     9901 gcggcttcgg cgtcgccggg cccggttagt gtgacctgtc ccttgccgtc gtatccggat
     9961 actgcagttt tcaaaatcgc cggacagccg atttgctcga tacccgagca caaatcatcg
    10021 agcgtattca ccggcctgaa aggagcgacc ggaaatccat tttgttgcag aaaggatttc
    10081 tcgcgcaagc gatgctgtgc aatgtgcagg actgagccgg acggtcgtac cggagcaaaa
    10141 tgcgccgcgg cctcgaccgc aaccgcggga acgttctcaa actcgaatgt caggacatga
    10201 acgttctcgg caaaatccct gatggcgtcg agatcatcgt aagccgcggt gatttcaaga
    10261 tcggcaatct gaccggtggg agtatccgtt tccggagaaa atgtgtgcac ccggtaaccc
    10321 atctgccgtg cggcaatggc aaacatgcgg cccaattgac cgctaccaag gacgccgacg
    10381 acggcgcccg gcaagatcgg cgccacagag tctctcacgg cagtttttgg cggcgaacgt
    10441 tatcgctttg tttcgtgcga aattgccgga gcttctcgcg caactcggga cgtgaattgc
    10501 cgagaatagc caccgctagc aacgccgcat tcgtggcgcc ggccttgccg atggccaatg
    10561 tacccactgg aatccctccc ggcatctgca caatcgacag cagtgaatcc atgcctttga
    10621 gcgcagcgct ctccaccggc acaccgagca cgggaaccac ggtctgagcg gcggtcatcc
    10681 cgggaaggtg cgccgcacca cccgccccgg cgataacgac ttcaatccct cttgtttcag
    10741 cagtgcccgc gaattctgaa agccatgccg gagtgcggtg cgccgaaaca attaagtttt
    10801 cgtgcggcac agcgaattct ttcaatgtgg aagatgcatg ctgcatggtc tcccaatcgg
    10861 actggctgcc catgataata ctcaccagcg gattgtttgc ctcaggcatg tggtttctcc
    10921 agcaccgcgg tcggttagtt ggtcactttg gcatacggcg aaatccgccg caaccacgca
    10981 ctgggctacg gccggtgcgt attcaggctt ctcgagcacg tcagctctgc gggggtcttc
    11041 tttcacttat tccactgcgg cgccgcacct gagctttggt taacaaatga ttgattagga
    11101 acactcaaac tgacctttca attgaaaggt ctttcgatga gatgatccga aatgacaaac
    11161 gcctcggctt caactgtacg cagcgatgat ctcttgcctg cgcgagcagg ttagaacatg
    11221 atcgttgacc attccggtag cttgcatatg cgcataacaa atggtcgagc caacgaattt
    11281 aaagccgcgc tgcttgagat cgttgctcag cgcatccgat tcgcggctgg atgcccgata
    11341 gtccttcagg gctcgcaacg tgttcacgag cggcttgccg ccaacgaacc gccagatgta
    11401 ggaatcgaaa ctgccgaact cttcctgaac ggctagaaag cttttggcgt tagtgacggc
    11461 cgccgctatt ttttgttggt tgcggatgat gcctgcatcc tgcatcaagc gatcgagctg
    11521 ccgtttgcca tagcgcgcca ccttttcagg atcgaaattg gcgaaagctc gcctgtacgc
    11581 tgcgcgcttc ctcaacacgg tataccagct caggcctgct tgtgctcctt caagtgtgag
    11641 aaattcgaag atagttcgat cgtcatggac cgggacgccc cactcacggt catgatacgc
    11701 aatgtagtcc ggtttagttg ggtcgaccca gggacagcgt tcatcggcgg agcaattctt
    11761 catagtggct tctgctaaag cacgattgca atcctacggg aaaatagcat caagacttcg
    11821 ggaaccgccg atagagagcc agtacccaca tcgccaaaga aaaaacaaac cccaacaata
    11881 tcgccatcaa ccaggactgc ggcaacatag gcggctcggc ccgatgcgca atcagcacga
    11941 caaagtgcag tccggtcata agaacgataa ccaggcggcc gagccggtgg ccgtgtgcag
    12001 cgaggaaatc caaagattca tgcagccgcc gcggcgcgag ccagtaatcg ccgttgggca
    12061 cattgagcag gtgaggaaat ttctgtgaga gcacgccgaa accggcgctg atcacaatcg
    12121 gaatgctgat aagaagagtc aaaatcagta acaaataagc gctgcgtgtc atccagccgt
    12181 cgggctcgtt gttcatgtta aaatgggtcg cgatcttgct gggaagaaaa tatgaggtgt
    12241 aaatcacaaa cgttaccgaa aacaccatca ggcaaatcaa actcaccagc gagcgccccc
    12301 ggcgattccc gtgaacttgc atcggcttaa tgagagatcc agtgatgccg cccggtagat
    12361 ctagccacga tccgcggggc aggataattt aatacgactg ctgcacatag gaccgaccaa
    12421 ctggcgccgt catttctgcg ctcaaggcaa taacgggcac tccagcggag aatagatcac
    12481 cacgcgattc gcgctagcct ttaaacaaag ctttggcgga acagatctgt ctgctatcat
    12541 gtcacgattt ttctattgcg aaatgaggtg acccttgaac ccgactcgct ggcaatactt
    12601 tcgagaattg aggccttttc tgatcgccgc cctgctgctc ttctctctgg gaatcatcgc
    12661 gggaaaactg ctggcgggtc actcgacttt tgccgggcta aagatcaacg aatctctcgg
    12721 tgggttcgcg caaatgttta taaaccttcc tcggccgatg ctggcgttga tgattttcgc
    12781 taacaatgct gtcaagactc ttttggtgat tgtgctgggg cttgcgtttg caattgttcc
    12841 gttcgttttt atcgttgtca acggggttgc aatcggtgtt gtcctccatc tagccaccga
    12901 atcgaagggg ctcgcctatt ccctgctcgc catcgtcccg catggtcttt ttgaactccc
    12961 cggagtgctt tcaggcgctg ccatcggtgt gatgttgggg aataaagcaa tcaggcgcct
    13021 gtttcgaaaa tctgaattta aggtcggttc tgaactaagc cgagccgtaa agatcttcct
    13081 aacgattatt cttccgctcc ttgcggtcgg agcgatcacg gaaacctatt tgacggcagc
    13141 gattctcggc aaatagccgc ggcagtatct gaactgcttg tccggcaagt taggatcaag
    13201 cgatcagacg agtgccgatg gcgaaggcat cgcagaatcg ctccagaccg tgaactccgc
    13261 cgttcaccaa ccggcgggcc aatttcagat catcttcaag cagagcctcc ttgatcaccc
    13321 gctccttagc cttcaagaaa ctggccagca atttgccggc tacatcacca tcgttggcca
    13381 gatccggcgt ttgcaccagg cgatcgccca atccgatagc tctaccatgg acttcgtagt
    13441 tacggcggcc ggtgagctga ataaagccgc gccccttgta ccgatccccg tctccccgct
    13501 tgctattgcc aagatccgtt cggaagtcat acaaatcata gggatggccg ccgggcgatg
    13561 tattgtattt cgatttgccc tcgcagatgg gttcaaaaca ttccgcttcc gcgcgaatcg
    13621 ttgctaatgc catcagaacc aacggcttat caaccaatcg ttccctacgc aatgcactta
    13681 gaacataggg cagattggcg ctgatgttgt cgaccggagt aaatggaaaa attttcgata
    13741 ccacagtcac agtcgcaccc ggcaatgctg acggcagcgc atcgctgcta actagtttca
    13801 atgccctcaa cgtgcgcggc ccggcgattc cgtcggccaa gagcgcgcta ctcttctgaa
    13861 aagcgatcag cgccgctttg gtcgcagggc cgaaatcacc atctatttta cctggattaa
    13921 agcctagttt cttcagctgc ctttgcagtc gaacgaccgc ttgcccatgt gaatcttccc
    13981 ttagaaccat cgttacgcac ctatgtcggc gggattgcct cggtctatta atctgacttc
    14041 ggaaagttcc ctttaaaagc catgagcgaa taatctcgag ccaacgttgc ggtcagagca
    14101 gcgatgcgac gcacgggata acccttcgct tctacgagat tgccccaaat cggcgccggc
    14161 caacaaggat cgtcggcgcg cctgccgacc acatgcagat gaagttgcgg cacgacgttg
    14221 ccgatactgg caaagttgat tttgctaacg gcgaattcag tctttatgaa gcgcgcggcg
    14281 attgccgctt cctccatcgc cgtattccgc tcagattccg gtaaatcaag cagatcaccc
    14341 ggtgatgccg tctcaggaac caggattaac cacggcaccg ccccgttctt gtgcagtaga
    14401 agatgacata gccggaattg gccgattcga tggcagtcat gaagcaattg agggtgaatt
    14461 tcgaaacgag acatgtggcg cgcgaaatta catctcttcg gcgggcgcgc cctgccgtcg
    14521 caactcatcg agcttctgcc gtgccattgc tttgagatcg atatcggttg agggccgggt
    14581 atcggctttt aagattttcg acatatatcc aagcgcgtat tgattatccg cttcggtttc
    14641 agaggctata cagtcctgag gaaccagcag attgaaatcg cgcatgtaac cgtcgctggc
    14701 cgtgaaaagc acgcaaatgt ttcccgcgat gccggttagg atcagtgtgt tggctttaag
    14761 gtagcgcagc aacagatcca ggctggtcga gtaaaatgcc gagtgcttgg gcttcaaaac
    14821 aaagtagtct cgctcctcgg gtctcagtaa ctgcgcaatc ggctttcctc tgacgttatc
    14881 ctctatgcag tgatcgacaa gctttcggaa atcagactgc cacttgccga agttgtcatt
    14941 gacatagatg accgggattc cggcggcttt cgcgttcttc ttcagtttgg caatcggttg
    15001 agcgatctcc agagtattct tgaacatctg atcggcgcca tcgaactcaa aatcgttgat
    15061 caggtcgatc agtatcaata cgacagggga ctcgtccgga acgttcccgt gcaaatcttg
    15121 attgttaagt tggcttacca caagagtcct ccgtcgatgc agctaggata tctgagagta
    15181 gcatacgaac agggatttta ttgcaccgtg aaggcgccgc ggcatcgagc gttgatcaat
    15241 gaaaatcccg acttgacgat ttagcaggct gggtgcttac tttgggttgc caaagtttct
    15301 ctgcaaccag atgaacgaca ccgtcttcga cctgaagcgt gccgctgatt cctagaaaat
    15361 taaccgtctt tgccaacacc gagtagcgct ggaaaatatt ttcccagacc acaacgttga
    15421 cgaagccggt ttcgtcctcc agagtcatga acacgatccc gcccgcggtt cctggacgtt
    15481 gacgacatat caccagaccg gcgtaatcga cttttgcgcc gtttcgttta gcggcgattg
    15541 aacgggcatc cggcaatccc tgggcggcaa gcatgccgcg aaacggctgc aacggatggc
    15601 gcctgacgct gtgcgagctc gtccgatagt cccaacccac ctcctcgaaa gcattcaatg
    15661 gctgaaatcc cgggtctatt tcctcagttg gtaagcgcag tgactggcct cttaactgtg
    15721 cccagcgccg ggtgtcccac aatgcagtac ggcgatcgac gccaaggcca tcgaaggcgc
    15781 cggcttccgc tagcaagcta agactgcttt cattgagatc tgtgcgccgg acaaaatctt
    15841 ccagcgaatc aaatgaacgc gcatgccgta tttgtgagat cctctcccaa tcgcgctcgg
    15901 ctaagccctt gacgtaacgc agccccatgc gcacggcgaa acctgcggcg ctgtcagcgc
    15961 agctctccag cgtgcagtcc caatcgctaa cctgcacatc gatcggtcga accaccactc
    16021 catgacgctt ggcgtcttcg acaatggtcg ccggcaggta aaagcccatt ggctgggcat
    16081 ttaacagaga acaggtaaat tctgccggat aatgacaccg cagccatgcc gtggcatagg
    16141 cgatcaaggc gaaactggcg gcatggcttt cgggaaaacc gtattctccg aagccgcgga
    16201 tctgttcgaa aactctttcg gcaaattccg gcgcgatccc tttggcctgc atgcgtgtga
    16261 tcaagcgctc gcgatggcgc tccatccgtc cgctacgatg ccatgcggcc atatcccgtc
    16321 gcaactgatc cgcctccccc ggcgtataat cggcggcgac cacggccaag cgcatgacct
    16381 gttcctgaaa aagcggcacg ccgagtgttt tttccaacac cggaactaaa ctctcatgcg
    16441 gaaactcgac cggctctttt ccctggcgcc ggcgcaaata aggatgaacc atgccgccgg
    16501 tgatcggccc tggtcggacg atgctgattt gaatcaccag atcgtaaaaa gtttttggcc
    16561 gaagccgggg caacatcgcc atctgggcgc ggctttcaat ctgaaaaaca ccgacggtat
    16621 cgctgcggca aatcatctca aaggtcgcgg tatccttgcc cggaatggtc gccatagaca
    16681 gctcttggcg gcgatgctcg cgcagcagcc taaaacagag attgagctgg gttaagctgc
    16741 cgagcccgag cagatccact ttgaacaagc caagatcttc aagatcgttt ttatcccact
    16801 gaatcacggt gcgtccggcc atgctgccat tctcaatcgg aacgatgtca tgcaccggtt
    16861 cgtgacccag aagaaagccg ccgggatgaa tcgacagatg acgtggacag tctaaaatct
    16921 cattactcag ttggagcagc tggttgtgca gccccaccgc cggatcaatg cccgtctgct
    16981 gcagtatttc aggccgaata tgctcataag gcgaaagaaa tttggcggcg cgatcgagag
    17041 aagtctcgga aagccccaaa gccttgccga catcgcgcaa cgccgagcgc gagcggtagc
    17101 gaacgatatt ggctgccatg gcggcgcgat cgcggccgta cttttcgtat acgtgccgga
    17161 ccacttcttc gcgccggtta tgctcgatat cgagatcgat atccggcggc tcggcgcgct
    17221 ctttggaaat aaagcgttca aacaacaagc cgatgcgcgc cggatcgacg gcagtaacgc
    17281 ccaggcaata acaaaccgcg gaatttgccg ccgagccgcg gccctggcag agaatgccgt
    17341 tggcgcgaca gaactcgacg atctcccaca tggtcaaaaa atagcccggg tagtcgaggc
    17401 tctcgatgac ctccagctct ttttctaatt gcccgacaat gtcaccgggg atctcttcgc
    17461 cataacgcca gcgcgcgcct ttaaacgtaa gctggcgcag ccactctgcc gatgtggtgc
    17521 catcgggcat gcgttccgac ggatagcgat aatgaatttc atccagtgaa aaccgacaac
    17581 gctcggcaac ttcgagcgtg cgcgcgacag ccgccagatc atcggcaaag agttgggcga
    17641 atagataggg agatttcaac ccgtgttcgg cattgggctt taacctgcgg ccgcaagacg
    17701 ccaccggtat gccatgacga atcgcagtca acacatcctg caacggccgg cgcgccgagc
    17761 tatggtagag aatctcattg gccgcgatta agggaaagag atatcgcctg gcccgctctc
    17821 gcaaccgaac ttcctgtccg aattcttctt cacgccggtg ccgcgcgatc atgccgtaca
    17881 ggcgatcgtc gaaggcgtcg cgcaccggac cgatgacatc agccggttcg tgttcagcaa
    17941 cgagcaggct cgaatcgccg ccccagaggg caagcaggtc gggcgcatgc tcacaaacct
    18001 catcccaaga aacgctgctc tccccttttt ccgatcgcag ccgtccggcg gttaaaagac
    18061 ggcagagatt ggcgtaaccg gcgcgattct gcgccagcag aacaatcgac gaaccatcat
    18121 cgatggagat ctgggagcca aaaatcaatc tgaacccaag ctcccgcgct ttaacgtgcg
    18181 cgcgaacgat gccataaacg ccgtctctat cggtcacggc aagagccggc aatccgaacc
    18241 gatatgattc ctcgaccaac tcgtccggat ggctggcgcc ctcgagaaaa gaaaaattag
    18301 ttttgcacca aagcggaacg taggaaggca tgtttttgtt ctagtcagcg ccacgagctg
    18361 ccgcatcaca agggctgccc agatcttgtc gtgataaacc gcggagccat tccctggtag
    18421 tagaagaata ttcgacgggc atcctcatgg caattgctac ctttaagtta tcggggaggc
    18481 atttcgcgct tgatcgacga gcttaactct ttcaatttca gctttcggca gcctgcctta
    18541 gtccgagagc cgaacccggg cagcctctga aatcgccacc accgccggat gcttaagctt
    18601 gcgatccaga gaaattgcat aaaaacgctc gcgaatactt cccactcgcc ccagggcctt
    18661 cacatcgtat tctcgtcgaa cttcggcgct gataatcgaa ggcgcgacga agagaccgag
    18721 accccgcgct ccaaatacct tcaacagagc gctgtcctcg aattccgcaa caatcatcgg
    18781 ccggatcttt tctgaatcga accattgatc cagggagcgg cgcaaggtgg tattgtgggt
    18841 gggcaacaaa aagggtgcgc catcgagaga ccgaggaaag ccgcggcgat aaaccttggc
    18901 caatttttcg gtcgcgaaca aagtcgaacc gcactcgccc agcaaatgat tgaatgcctt
    18961 cacgttgacc gcgggatcaa tgggggcatc agccaggacg acatccagat catggacggc
    19021 tagcgatggc agcaggtcac tattgctgcc ttcccggcag ataatccgat aggattcctt
    19081 taatcccagc gctggctcga tcagcttata cgcaactaat ttaggcaaca catcggcaac
    19141 gccgaccgtc aaacgggcga catgcccccc ggcgcgacct ttaagggcgc cggtcaattc
    19201 ccgccccaag gaaaaaatct cctcggcata acgaaatacc actttgccca tatcggtcag
    19261 agttagatgc cgcccggtct tgcgaaaaag cttttcgccg atggatagct ccaacgattt
    19321 gacttgggca ctgatcgtag cctgggtcaa gcggagttcc tcgccggcgc gggaaacgct
    19381 gccgcaccgc gccacattcc agaaataaaa aagatgatgg tagttgagcc attccatgac
    19441 ccggatgata gttcggtttt ttctatggat caatatgaaa ttattagttt gtcgaataat
    19501 cacgcaaagc ttatggtaat gcgtcgaaag gaaaccaacc ccatgacagc aattcgcagc
    19561 ttgttttctt cagaagattc cgggcgcctg tttgaaccgg atacggttct cggcgctcaa
    19621 ttctatgcaa cctataaaaa cagtccctac aaacagccgg agctccgttt gatggctgcg
    19681 gtgttggagg atgcgatctc ctgcctatcg attaagccgc gtcctgccac cgcgcgacac
    19741 agaaaacagc ttgaggaagc tcggcagtgg tttgccgcag aagatgagag cgaatggatt
    19801 ttttcgttca agaacatttg cgaagcgctt ggaatcgatc cagcctacct gcgccgcggt
    19861 ttgatccgat gggcggcaac cgaactgggc tccgacctac ctaggatcag aaccccctcc
    19921 tcagagcccc ggagcaaaat caggctccgc gccggataaa agaccgcccg gaattctcgt
    19981 gagtaaattc gttatcagtc gcgagcgagt gagttcagcg ataactcgga agctccagca
    20041 acccggagcg atgaatgatc gttcctcctt accaccagca ccggttcgaa tgcctcgcac
    20101 attgcagccg acagcgctgt cgcaagattc tcccttaacc gttctgtgat attgctgttt
    20161 aagatcctgg cccaattcca tctgtttcgc tcctgcgcca ttcgcatgtc gcataccttt
    20221 ctgctttcca tccatccgcc tttacgcgtg gcgcttcaag cccatcgatc aagctcttgt
    20281 cacgcgggat tgtcttgaca tcgggcgaac cagaacgata gaagaggcct gtcatcgtta
    20341 aacgagtagc caaaggagac cttatgtcct cattctttcg aaaactctgc ctgctcatgt
    20401 cattgccatc gatcatcctg gtggccagcg cggagcggcc ggcggctcaa acccgggtgt
    20461 cgatcggcgt tacggaaacc attgagactc tgaacccgta cggagacagt gtctcgcttc
    20521 tgtacggaat ttggagcgaa atcaccggac cgttctgcac ctacaattat gacaaaggag
    20581 atttcgaagg acgtctggcc gaacgttgga aggtggagaa tccgaacacc tgggtattct
    20641 atttaaataa aagctatcgc ttcaacgacg gatccgaggt gaccgccgat gatgttgtcc
    20701 attcgatgat gaatcgggtc atcaaggatc cgcaatccaa gcagaaagca tcggtggccg
    20761 gtcccattat aaaggcagag gccgtcgata agcatacggt aagatttacc accgacaagc
    20821 cttcggcgcc gctgctcggt tttgtttgcg accggctcat cgtcacgagc aaggcgatgt
    20881 tcgacaagtt cggccgcgat atcgccgaca aggagcatat gctcggcgca ggtccctacc
    20941 ggctcaagga actcattccc ggccagcgca tggtcatcgt caagcgttcg gatcatccag
    21001 aggcaaagaa aaatccgaac gcgccggatg aaatcgttta tcgtgtcatg cgcgagaccg
    21061 agcaacgggt caccgcatta ctcaacggcg agattcagat tgctcaattc attccgccgc
    21121 acttgcgcca gcgagtcgag aaatcgccta acttaaagat tatcccgacc gactcggttg
    21181 agatcatgtt tctcgccatg caacccaagc ctcccttcga taaaaaagaa gtgcgccagg
    21241 ccgtatgtca tgcaatcaat cgcgatcaga tcatcagcac tctgttggaa ggcttcgcca
    21301 gccgtctcga cggaccgatt gggcccggtc agtacggcta cgaccccaat ttaaagccga
    21361 agatgagcta cgatccggaa ctctcaaaga aactcttgac gcaagcgggc catcccaatg
    21421 gcgttgacgt ggaactgcag actccggtgg gacgctatac actggacaaa caactcaccg
    21481 aagcgatgat tccgatgcta aatgccgcgg gctttcgcgc caaactctct actcccgagt
    21541 ggccgacact ttgggcgaat gttcagaagg gaaccgtgcc tttttattac atgggtcgtg
    21601 gctcggttca ggatcccagc gctgcgctgc accagtattt tcattcgaaa gagacgccgc
    21661 gaatcggcta ttctaacccc aagctcgatg aacttttgga caaagaacaa gccgagttcg
    21721 accccaaaaa gcggcggcaa tacctgaccg aggctatgag tctcattacc gatgaagcgc
    21781 ctgcttgttt catgtggcgc cacaagctgt tgtgggggat gaacaatcgt atcgattaca
    21841 aaccgctgcc cgacgcgcgg atcttcggcg tggatataaa agtcgcaaaa aagtagccgt
    21901 tgccagctta gtgggcgaac caggattata atttctgaac caaaggcgca ggcatgaccg
    21961 gagaaaaatt cgtcgatgta gatggcataa gaacacgcta tttcgacaaa ggtaacggac
    22021 cggttgtcgt attgttccac ggcggtcatt tcggttccaa cgatgcggca gattgcgctg
    22081 aagactgggg cctgaacttt gacggactgg ctaagtggtt ccacgtttat gcggtcgaca
    22141 agattggcca aggtcatacc gacaatccta gaacggatgc cgattatacg atggcggccg
    22201 ttgtccagca tgctcacggt tttcttaagg ctctcggtct gcgcaacgtc catcccgtag
    22261 gacattcgcg gggcgcgtat ttggtagccc gattgacctt ggaacatccc gagctgtttc
    22321 gatcgtgtat tttggccgac acgaacacgc tggcgccggg ggtcagcaaa aacgagatgg
    22381 tgatggcaaa cccgccgctc ccacggttga gccgagagag ccaacggtgg gtgctgcagc
    22441 ggtactcctt cgcctttgag cacatcaccg aggaatggct ggaggcgatg atgcgggttg
    22501 cggcgctgca gaaataccag gaagcggtgg ccaagatgga aaccgccggt ctcaggacaa
    22561 tccgctttct accccatctg gctaggcaga aggacgaaac actaggatgg attagggagc
    22621 gcggcttggc taagccgacc ttgctggttt ggggttacaa cgatcccacc gcgacgttag
    22681 atcaaggtca ggctttattc gatctgcttg cgcgcagtac gccggattcg cggatgcagg
    22741 ttttcaaccg ggccggtcat tttacctatc gagaacaccc ggtcgaattc aatgagctgc
    22801 tgcgatcgtt tatccagcga aacagcgggt aaacgtgtac agatggcgag aattttcgta
    22861 aacctttgaa agattcgata aggtggcaag ttatctcctt catcgactcg ctggcaccat
    22921 tccagttctt cttctcatta gtctgcttgt tttcctgctg attcatgccg cgccgggcga
    22981 tccgactttg ctacttttgg gcgaggagac caatgctgcg gaagtcgcca aggccaaaga
    23041 gcgctgggga ctggatcggc cgctgcacgt tcagtacgtc aaatttctga gttccgcagt
    23101 tgccggcgac ttcggtaagt cgtttaaata cggcgagccc gtgaccagtg tcatcaagtc
    23161 gagactgcct gcgaccatag agctcgcggt cgtctcgatc atcatcgcga cttgcctggc
    23221 tattccttta ggggtatggg ccggctcccg gccgaattcg tggctcgaca acttcggcac
    23281 gacattcggc cttttcggga tcagcatgcc gagcttctgg cttgcgatca tgctgatctt
    23341 gctgatggcc gggattctca atctcctgcc aacctccggc cgcagtacct acggcgtggc
    23401 tggaccggaa cagaccggat tctacatatt ggacagtctc ctgcagcaaa actggcaagc
    23461 cgcttgggac gggctcaggc acattttact gcctgcctta gcgcttggcg tgaatatgct
    23521 tggaattctg atgcgcgtta cacgatcggc aattctggaa gtcatgaacg aggagtacgt
    23581 caccacggcc cgggccaagg gcttggcgga acgtaaagtc gtctggcgcc acgtcaccag
    23641 caatgctctg ataccggtga tcacagtggt cggcctcgag ctaggaacgc tgctgagcgg
    23701 ctcgatcatc gtagaaaccg tgttcgcatg gcccggcagc gggagcctgc tcatcaccgc
    23761 tctcaatgcg cgcgactatc ctctggtaac cgggctcgtg ctgagctata ccgtggcctt
    23821 tgtcctaatc aatctcatta tcgacggact gtatgcggtc attgaccctc gaatccgctt
    23881 ctgaaaaaaa cccgggcgtg gtcgcgagcg ccttagggcg gcttcacgcg aaagcattca
    23941 gcggcggctt gatcgtctta ctgtttattt tcttgggtat ttttggtcca tcgctcgctc
    24001 cctacaatcc aaacaagcag gagctcacgg cgatgatgaa gcctccgcaa ggcgtcggca
    24061 gcgaacacgt gctcggcacg gacaatttag gacgtgacat tttaagccgg attgtttatg
    24121 gcgcgcgcgt ttcgctgctc gtggcattcg cggtggtctt cgtttccggt tttatcggta
    24181 tcggcctagg cgcaatctcc gggtacatcg gcggcaaagc cgattttctt attcaaaaat
    24241 tggtggaggt cgtttgggcg tttccgccgc tgttgctcgg catcacgatc atggccttct
    24301 tgggtcaggg cctaaccaat ttgattattg cgctcgtggc gcaacgctgg attccgtact
    24361 gccgggtggt gcgcggacag gctctctcgc tgcgcgaacg cgatttcgtc atggcggcac
    24421 gttgtcttgg cgccaccaaa acccagatta tcgggcgcca cattcttcct aatctgattc
    24481 aaacatcgct ggtcatcggc acctttgcga tggcatcgtc aatcatcgct gaagcgagct
    24541 tgagctttct cggcgtcggt gtgccgccgg agattcccac atggggtacg atgcttgcgg
    24601 atgcgcgcat ctatatcagc acttcgtggt ggatgccgct gtttcccggc ctgtgcatct
    24661 tcataacggt tctgggcata aacctgctcg gcgatgcctt gcgcgacatt ctcgaccccc
    24721 ggctaaaacg gaccgggtcc ggggtgtaag tcttacctcc gtcagcaatt tggttaaatt
    24781 catcacaacg aaccgaaaac ccacctaaag ctgcgctttc attgattctc ttaacaactc
    24841 gtgtaatttt actaaaatgc gtagaaccaa gatcgtttgc accattggtc cggcgagcgc
    24901 gtcaagaaaa gttctcacag gcctaattca agccggtatg aacgtggcgc gcctgaactt
    24961 tagccacggc gactactctt ttcacaaacg agtcattcaa aacatccgcc aaatcgcccg
    25021 ccggctcgat aaacctgtag cgattttaca ggatctagcc ggccccaagc tccgtctcgg
    25081 acccgtagct gccgaccgtg tacgcttggc ggcccggcgc cctttcgtcc tcaccacccg
    25141 gccgattctt ggttccgagt tggccgtttc cgtcaactac aaaggcttga ctcgggccgt
    25201 aaaaaaaggc gatccgattt tgctcggcga tggtgagatc gagctcgagg cagttaaagt
    25261 cggcgcccac gaagtaactt gcaaagtgat cttcggaggc atactgggat ctcacaaagg
    25321 catccacttt ccgcgcagca gtttggatgt gcgctcgctc accgctcgcg acaagcagga
    25381 cctcgcgttc ggcatcgagc agaaagtaga tctggtggct ttgtcttttg tgcgtaacag
    25441 cgaagacatt gtctatgcgc gccgtgagat gaacaaccgc ggcgcctcga tcccgatcat
    25501 cgctaaaatc gagaaacacg aagccctcga gcacattgac ggtatcctcg aaaaagtcga
    25561 cggcatcatg gttgcccgtg gggacctggg tttggagatt gcaccggaga aaattccggc
    25621 gatgcaaaaa atgatgatcc gcaaggccaa tgctctcggc aaaccggtca tcaccgcaac
    25681 gcaaatgctc cgctcgatgg ttcagaaccc gcgcccgact cgggccgagg tcgcagatat
    25741 cgccaatgcg gtgttggatg gcactgatgc gctgatgctg tcggaagagt cggctgccgg
    25801 cgactacccg gtggagtcgg tgcggacaat ggcccgagtc gccgaagaga cggaacagat
    25861 tctcgagccg cgctaccaat ttgccggccg caggcagagc gttcccgaag cgatcagctt
    25921 tgcagcgatt tcgttggcgc gggacttgaa cgtcaaagcc tttctcattc ctacttcttc
    25981 gggcagcacc gcgcgaatga tttctcgtta ccgaccgcct catccgatca tcgcaatcag
    26041 tccggaaccc cagaccgtta aaatgctttg cctggtttgg ggcgtctatc cggtgcaggt
    26101 gcctgggttc cagagcaccg acgtgatgct gaaagtcgca cagcaaaaat cagtcaagct
    26161 cggcttcgtc aaacgcggcg atcttgtcgc cataaccgcg ggtctgccgc tgcaccaggc
    26221 gggtaccacc aacatgatca cggtcaaagt cgtcgagtag agtttcgccg gcaagcatac
    26281 gattcccgcg ttcgacgctt cgcatggatg ctgatttttg ctttcggaac ctgatgcacc
    26341 taatcttgat ggatgccgtc ggctgtcgtg ttgaaaaagc acgattgccg cacctgcatc
    26401 gctctctcgt gttccatatg gctttgccgg taagttatgc gtctcttcgg tatcgatcgc
    26461 gcgccttgca acgcttacgc gaactgggct agaaccagac tgaatgatca tggaggtaaa
    26521 gccatgtccg aagcactgaa agccttcgaa gatgagcttg aaagtaaaaa cttcaaaggc
    26581 tactggcaaa acatccaggg cgatgtttat cgagaaccgg tgccgtcgta tgaaccctgt
    26641 cactggaagg gcaaagagct gttcgccgcc atcgaaaaag ccggagaagt tgtcggactc
    26701 gatttgtcgt ttcgtcgagt cattcaactc tggaatccct cgctgaagaa cggcacgacg
    26761 cggaccttgg tattgaacct gcagatgctt aagtccggcg aggaagctct tagccatcgc
    26821 cacattgccg gtgcggtccg ttttgtcgtt aaaggtagcg gcgcccggct gatcgtcgaa
    26881 ggggaatcct tcgaaatcgg cgagggagac tttgccacaa cgcctagttg gacctggcat
    26941 gatcacgaga acaacgcagg tcaaaccatg ctgtggctcg atggtttgga ttcgccgctt
    27001 gttcgactgc tcgaaaccga cttccacgaa cccgatccgc gcaaaaagca gacggtaacc
    27061 aagcccgaag gttattccgc atcaaccctc ggagccttgc gtccgtcatg gatgcgcgcg
    27121 cacagcctcc agcctccggc gtttgcctac aagtggtcgg acactgaaaa cgccttgaat
    27181 aagatcggcg agcaacccgg cgacccctac gacgggatcg tattggagta tgccaatcca
    27241 ctcaccggcg gccccacttt accgaccatg tcttgccaga tccacatgtt acgctcaggg
    27301 gaaacgacca agagccatcg tcacacgagt tccacgatct attatgttca tcgtggccag
    27361 ggcgtgagca cgattggcga caaacgctat gaatgggaac aaggcgattc gttcgtcgtg
    27421 cctctctggt atcaccatca tcatgagaac accgggaaag atccggcaat cttcttcgtg
    27481 atgtcggata agccgcttat ggacgctatc ggtcattatc gtgaacaaac ctaggcgcgc
    27541 ttgccggctg cccgtcaaaa gattgagata ctctagctgg aggaagcagc ccccatgttc
    27601 aatctagctc accgactcag ccccgacgaa ttcatcgata cctttttcga agtcaataaa
    27661 cgggatcacc aacgcccgcc gcacccgtgg gacaccttgt ttcgcaatgg cgagtgcacc
    27721 aaggagcagc tacagggttg ggccaaagaa cgttactatt tcactaaaca ggtgcccatc
    27781 aaagaatact cgattctgta caactgcccc ttcccggaag tccgccgatc ctggctgccc
    27841 aaagcgatcg aagaagaagg agaagatctt atcggcaggc ctggcaagcc tcatcctgaa
    27901 tactggttgg atgtgtgcga agggctcggt ctcaatcgtg aatttgccag aaactcagaa
    27961 ccgctttatg gcgtaaagtt cgcggtcgac agtttcgcca atgcggcgtt caaaaactcc
    28021 tggttgatgg gcgtcgccgt ctccgaagga gacgataccg ccaaggccat gattcgcgac
    28081 ctggaggttt tccgaaaaca ctacacgtgg gttccggacc ctgcgttgga attctataag
    28141 ctccatgccg gcgtcgacgt ggagcatggg attctgcgga aagagatatt gaaaaaatat
    28201 gccaccacga aggaactgca ggaagattgc atcaacgccc agctgatgaa aaataatatg
    28261 cgccgcgtca tggccgattg tatctacatg gcgtatgtcg ttcagggcat gacttgcgag
    28321 tcgagcgccg ccgcgtagtg cgaacgcgag gggtcccgcc cattacatct cattttggcc
    28381 gttcactggt tagatcactc gctcgatatt agagttcgga ggggtttgcc ggcccggttc
    28441 gattcggcgg agactaatga tgatccgatc ccgcaccacg atattactgc tgctgatcac
    28501 cgtcgtgctg ccgaaccgag ccggcgccgc tcccctgcgc atcgcttata gctcgatcag
    28561 cggtgccatg ctgccgctgt gggtcgccaa agacaaaggg cttttcacca agcatggcgt
    28621 cgacgttgat ttgacttaca tccgaggcgt ttccatcgaa gcgctgctag cgggcgaagt
    28681 tcagtttgtt cgcgcctcgc cgccctcggt tgttcgctcc gcgctccgag gctccgatct
    28741 cgcgtttatt gccaatacca tcaacgttgc ggttttttcg ctgatgtcga gacctgaact
    28801 gcaaaagcca gaagatttga agggtaaaaa gatcggcgtc accaatcttg gcgattcgcc
    28861 tgatctggtt ttgactttgc tgctggataa atgggggttg cagcgcggca aagatcttgc
    28921 ggttttagga atccgtggcg gcatgcccga actgctcatt tcagtcagca agggattcgt
    28981 cgatgcaggg atgatttcgg cgccaagcaa tctgcgcggc atcaagatgg gcctacgcga
    29041 gtttgtcgac acagcggatt tgggcattcc gtataccaat tcgccgctca gtacccggcg
    29101 ctctacgatc aaaaaccagc gcgacacggt cctgcgcgtg ctaaaggggt actacgaagg
    29161 ggtacaagag acccgacgtg atcggaatgg cgcgatgaaa attcttgcaa agtacgtgcg
    29221 cctcaccgac cctgatatct tgttcgaagt ttatcaaacc tatggcgcca aactgctaca
    29281 gcctgacatc aaggtcgatc tggacggcgt caagggactg ttgaaaggtc taggaagcga
    29341 agcagtggga ccgaacccgg ctacgttcgt cgatttgtcg ttggtcgagc aacttgaacg
    29401 agagggattt atttcaaccg gggcccggtg actcgagcta agcacgaaac acggtcttct
    29461 cgggtctttt tctgcggttc cgagaagacc gttagaaggt tactatactt ggttaacgcg
    29521 cgctctcttt ggatccgctc ttcgccctgc ttctcgatcc gctctttgcg tcaccctttg
    29581 attcggtttt gatctgcttc tctgttgatt gggcaagctc aagatgcttt ttcaggacag
    29641 gcagggtttc gctggcgaac tttttcaaat cggggtcctt acctttatcc gcctgcgttt
    29701 gaaatttttc gatatcctct ttgtgatcat ctaccattgc gtccatatac tcgcgatcga
    29761 actcggcgcc ggatagtttg gtcagcttat ccatggtcga tttgtgctcg cctgaaggct
    29821 cggtgggaag atctacgcct ttgccagagg caatcttttt taactgcgtg ttagctttgc
    29881 tgtgatcggt ctgcatgcgt ttgccgaatt ctttaacccg ctggtgcgat cccttctcag
    29941 cggcaaattt gcctaactcc acttccatga gcccgcccga agcggcttct ttcaggaatc
    30001 gctcttcacc gctgggaagt ttgcttttac tctccctact gctctccgcc tttgtgctgc
    30061 tactcgccgc taatgctaac cccgtgggag agatagcgac ggcggcgatc atcatcagtg
    30121 aaattttagc tactcgtatc atgacttcgc cctccgttta tattcattgt tatagacaga
    30181 gttgagcata gcatatgcca taaacctggt tagacctgct cgaaacaggt cgacgatttt
    30241 tccagcagga taagtgctaa tcggacaatc tatgaacgtt gtatgaatca gtctcaggac
    30301 aggcatacgg cactcgtcac ggctttggga caggataact caccatttgt gatactgatc
    30361 agaaagctcc atcaaaaaca tgcgcaacga gcgccggtgg acgccgcgca tagacactga
    30421 tggttgaata caaggcgacg cactggctgc ccggtccttg cacgttacgg tttccacccg
    30481 gctttcgtga gttgctggtc tgcttggcgg gcgaaactaa agtcataggc tcgctgaatc
    30541 ggcacgactt cggtcactcc cgctatctca cggacgatgg cgaggtcatt gcgttgcgcc
    30601 tcttcgtcga cgactccgtt tgccgtaaaa gtttttgcga tatcgtcata catgcgtatg
    30661 acgagagcgt tatcgagctc ggtaaatttg gttgccgcgg caattgcccc ttcgcgattt
    30721 tgccgcacaa aacgcaatgc tttgaccacg gcgcgagcga aaccggccat caccttaggc
    30781 tgttctttga tgaaccggtc cgaggtcgca atggtgcccc aggaattttt cacttcctta
    30841 cccataaaca taagatcttt cattccttga tcgatggcag cgtaacgttc ttcggggctt
    30901 aagatggcgg catccacggc tccggacaga agcgaaggca accgattgac cggcccggga
    30961 tcgacaaaga ccacatcttt attgccgtcc atgccgtatt ttcccatgat gtttctgaac
    31021 atgaacgccg cgatggaggc aacgcccgtg gttccgacct ttttaccttt aagatccgca
    31081 atcgatgaga tgttgggccg cacgaccacc cattgcagaa cctgatcatt cgcagcgata
    31141 acagttttga aaggcgcgcc gcctttggaa attgccacca gcgcgctgct acccgagcca
    31201 gaaaattgca cgttgccggc ggtcaacgcc tgtagacccg gcgcattttg catgtaaaca
    31261 agcttaaggt cgatgccctc ttccttcagg tatccgcgat cctgagcgat gggtaaaatc
    31321 gtgtactgaa aagccttcga ggtcagtgct accgtaactg tctcctgacc gaccgcaagc
    31381 cccgacgaaa tgcccatgca catcacgaaa gaaagcgacc acggtaaaaa cttaagcagc
    31441 attgaaatgc acctcctgtg acgttgatca cgtcaacgtg tgtaagtcga tggatatctt
    31501 tttaaaagcg cgaaccttta tccttcaaaa aaactgcgcc ctaacctact atcagctcat
    31561 tgtttccgtc aaattgaaat accccgaaaa aatcactgtt cagcaccgca ttgacatgac
    31621 agctcaggct tggataggct gactgcagaa tgacagggta atggaaggag ctgcaatgaa
    31681 agccattcgc atccacaatt tcggacc
//
LOCUS       DASUVS010000271        43144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_110131, whole genome shotgun sequence.
ACCESSION   DASUVS010000271 DASUVS010000000
VERSION     DASUVS010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 43144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43144
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110131"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VF182_14905"
     CDS             complement(<1..399)
                     /locus_tag="VF182_14905"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX6438425.1"
                     /translation="MRILVAGPGRAAGWAQLVDKIGALGAASVGIDMIFAEPDETPPT
                     LTQQTTKTLAEPRAASGALTPNDAALAAVLGRGVFVLGYQMRFGVERAEGRSCMLHPI
                     PVVSLQEPGAMAQPGLFESSATLCSLSGLGK"
     gene            1261..2265
                     /locus_tag="VF182_14910"
     CDS             1261..2265
                     /locus_tag="VF182_14910"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438426.1"
                     /translation="MLKPKTIATLLITAGHCLIAAPRLDAADAALKKLRIGYPSHSAS
                     MYPIYVTKEAKLLEKYGLDGEMIYVQGVQMVQVHVAGQLDVTTTSGLVTLQSSVSGAD
                     LILLANSIDVHLMKLITHPGINAPADLKGKSIAVTRFGSLTDLIVRPLLVSWGLDPKK
                     DVTLLQIGSQRDIATAIALKKVDAGVLSFPTSYYAERTGLKTLYDFTESGSEIPTTTV
                     AISREYGKKNRDSVLRFLRAYLEGHKKLQTDRALSIRALRRYGGIADEETLAYTYDLF
                     STRIVRKIPTISTQSVDNALKLLAETMPKAKERKASEFIDTSYMEELERTGFIKSLWP
                     "
     gene            2943..6266
                     /locus_tag="VF182_14915"
     CDS             2943..6266
                     /locus_tag="VF182_14915"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX6438427.1"
                     /translation="MPESTSNGGTSNDSTVLAVQSGSAAVRLREAGRFLDQFEPGAEV
                     LLLGASRGSVDDLARAIANERGATFGLHRLSFTQLAARLAALELASRDRAPTNALGHE
                     AVATRAAFEAGSEEAMEYFSPVSRTPGFPKALSRTLLDLRLAAVESGDVVRLPRSGTD
                     LAGLLDRVESLLNEAGASDRAALFEIATEALGSPVIGWPPMPLLLLDVPFQSEIETQF
                     LWTLLKQSPQVMITVAAGDVAALTQLEKRGARVQLIEEGEETDLTQLTRYLFSKEPPR
                     RRTRSGELVWFSAPGEGRECVEIARRILKEADRGVRFDEIAIVIRPAQQYAGLLEHAL
                     TRAGIPAYFDRGTRRPDPAGRAFLAILSCATDNFSAKRFAEYLSLAQVPSVDDSGKVP
                     VRSGDSTWVASRDDIFGILSEAAFDQADHGDVEEDEGLLTAGSVDGDRAVMAGTLRAP
                     WKWETLLVESAVIGGSERWARRLNGLAAEYELKIRALTFEQADSPRIAHLERERQNLA
                     HLQNFALPLIEAMSAWPQQATWGDWIQRLEEFAPRVLRRPEFVLRVLADLRPMGAVGP
                     VTLAEVRAVLADRLATLELEPPAHRYGRVFVCGADQVRGRAFRVAFVPGLAERLFPQK
                     LHEDPLLLDDLRREIGGGLTLQEDRAEHERLLLRLAVGAAAERLYVSFPRIETLDARA
                     RVPSFYALEVMRAVTGRIPDHHLLESEASEEANASLGWPAPQRPEDAIDDFEHDLSVL
                     RLLMRSDKDVKGHAHYMLRLNDCVRRSASERWARARPPWSFYDGLVRITDATKPFLQS
                     QRLTVRPYSVSALQNYAYCPYRFVLSAMYRLEPLEEPEPLERMDPLTKGRLFHEVLAE
                     FFRALQQSQMTLPASPMAALLELLDVTLTRVAANYAELLAPAVERVWQDEIASIRTDL
                     HIWARDLTQSSGWEPWLFEFGFGLPDAPGRDPNSRREPVKIDGRFILRGAIDLIERKP
                     GTNLLKITDHKTGKNRSERGSLIGHGQQLQPVIYSLAVEAATGLTVESARFSYCTTAG
                     GFSEHTVAINEPTRRMGIEALEIIDRAVEFAMLPPAPAEKACAYCDFLSVCGPNQERR
                     ARRKSRKEIADLIELRGKQ"
     gene            6263..9811
                     /locus_tag="VF182_14920"
     CDS             6263..9811
                     /locus_tag="VF182_14920"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX6438428.1"
                     /translation="MTLPPLADQGDRDQIATALDDTLIVEAAAGTGKTTELVRRIVRV
                     IQTGRAEVTGIVAVTFTEKAAGELKLRLREALEEARGSFGNDSEERRRLDTAVGRLEE
                     AQISTIHGFCADLLRERPIEARIDPLFRVLTEAQAVSMYDEGFHRWVEIELADPSAGV
                     QRSLRRPVFGGFGAQADGGDGPVDRLRRAGWELIQWRDFDGDWQRPIFDRLGHIDQLV
                     QLLNEFTELLEPPGDIRDALYADTWAARKLAEEIRRTESVSARDYDRLEAGFIELSRN
                     RNFKDARKGSGKNYRSGVLREDVCRACENLNHVLGDFKEEADADLAAALRDELRGCVS
                     AYEAAKAKAGALDFLDLLLKARDLIRDNAEVRSTFQARFRRIFVDEFQDTDPLQAEIL
                     LLLAADDAAATNWRETRPVAGKLFLVGDPKQSIYRFRRADVGIYRSVYEMLQAAGARR
                     VTLRTSFRARPNMQRAINAAFEPVMNGDGDVLQPAYVPLEPFRPEFPDQPSMVVLPVP
                     EPYGYRRLSNVSIEKSLPDAVGGFIDWLINDSGWKVAERPVTAKSSLAPTAARGFRPG
                     ETEHLVPIQPRHICLLFRRFTSFGEDMTRPYVEALEARGIAHLLVGGRSFHNRAEIET
                     LRAALAAIEWPDDELSVFATLRGSLFAVGEEDLLEYRHRIGRFHPFRIPNEIPANLGA
                     IVHGLKSLQALHRRRNRVPVATTIAALLEATRAHVGFALEHGGEQVLANVAHVVDLAR
                     RYEAEGGISFRGFIEALREQAENGEAGEAPILEEGSDGVRLMTVHKAKGLEFPVVILA
                     DMTAKLRASAASRYLDSERNVCAIRLGGFAPADLLDHEDEELLRDEAEGIRVAYVAAT
                     RARDLLVIPAIGDEEREGWIQPLNTAIYPAKEARREQIQAPGCVEFQSKDSVWVRPGG
                     SAASASTVSPGLHVCRRLGLSQIAEFSVVWWDPRALKLGVEAPLGLRRPELIVKDVEP
                     AIVESGLAAYNSWRERRQQAIAAGSRSSVALQTVTQAATSGRQPAPDLKLAPVEVIEL
                     PRAANRPSGRRFGTLVHAVLASVPLDGDVSIIQRLATIHGRILGASQDEVDAAGESVR
                     VALAHGIFEDARQAAQQGRCRREVPITWRDYEGLLIEGVIDLAFERAGAWTVIDFKTD
                     EEFRGDEPAYRRQVGMYAAAINAANGAPVAALLMRV"
     gene            complement(9913..10488)
                     /locus_tag="VF182_14925"
     CDS             complement(9913..10488)
                     /locus_tag="VF182_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438429.1"
                     /translation="MKKFNLVYAGLLAALLAAPTAASAQPHDKGGPDRREASLLGRWY
                     SNGERDKPVEIRTARRGLQARNEHGATSRLEAHGNNVRALDWEGGLRGHIKRDTIEWA
                     NGSMWTRAPSHRRDLAGTWYLNGARDKRVEIVSSRDGLQARNERGQTSRIQTARDGDI
                     RALDWEGGLRGDVRRDRIDWQNGTTWTRSPQ"
     gene            11209..11628
                     /locus_tag="VF182_14930"
     CDS             11209..11628
                     /locus_tag="VF182_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438430.1"
                     /translation="MAKLTFLLGLCGSGKTYIAERLATQTGADLFSDFLQDQSKNLPL
                     LLKNLSSGIDCIIDEWKYSIPRFRDKILVDLVNVPNLQVEWICFENDRANADWNVVHR
                     TNKADAEGHLRINEFLSDVYQYPAQAQILKITRIDAG"
     gene            11735..11974
                     /locus_tag="VF182_14935"
     CDS             11735..11974
                     /locus_tag="VF182_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438431.1"
                     /translation="MDELTALSILANTIDSCLNNDLPAAEVFAALNFLAARLPVKWPF
                     VEFRQGLDSGDETIRHQILKSALAAIKLLLRARQR"
     gene            12056..12220
                     /locus_tag="VF182_14940"
     CDS             12056..12220
                     /locus_tag="VF182_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438432.1"
                     /translation="MESCKCTARGHGHRNNQCDNAAATADGYCQTCSEKIGKVAQESE
                     QSGPKSPMGQ"
     gene            12569..13579
                     /locus_tag="VF182_14945"
     CDS             12569..13579
                     /locus_tag="VF182_14945"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438433.1"
                     /translation="MWRSLTRLFAIVAFFVIQPDTVFPQTRPKVVVGYASMSTVATTL
                     WVAQERGFFAKNGIDVQPIFIPGSPTLIASLSTGDVHFGYTGGTATLGAAVGGLDIKM
                     IAAFANHVQTDLVVRPEIRTPADLRGKRIGVTSIGGTGWMSAMLGLEQIGLNPDRDNI
                     LLAAFGDQRVITQALETGTIQGASLAGVFGQRLKRAGYHFMGELEKIPLVGTSIVVKS
                     DYLAKQQSIVRNTLKALIESHAFVHNPSNKAAVIEITSKKLGIADPIAASDGYDDYVR
                     RTDRKPFIAIEGLKNIQRFMKLRNPKIAEVNLGRLIDESLLRELDKSGFAEQSLGGTT
                     TR"
     gene            13625..14716
                     /locus_tag="VF182_14950"
     CDS             13625..14716
                     /locus_tag="VF182_14950"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438434.1"
                     /translation="MAIDGMKVIDLDSHLVGDLESWDQTIEAKYKDFLPRKLPTRDNE
                     RRKTLVGNQIMIGSELGRQKAEKKEWVTPNDLTAHGRVRNMDLDGIDVAVLSPNSPAL
                     DILWFVDDPELAAAYARAQNNYMNYYASQQAGRLMWAGVIPLQDSKEAIKELHRSREM
                     GSKALNVKATPIPGKEWSDPHFDPIFDELEKTNTPIIFHDTKTGSMGHERFADNFFFS
                     HMVGRTIESMVCLMIYLCGGVMEKHPNLKIICLETGASQMPWWLSRMDEHWEKLPHLV
                     PWQKRKPSDIFNESVYVGCEPFEDGLFEWAVEYLGGDRLVLATDSPHWDSSQPGQVTG
                     PVAKSTKISHDNKRKVLGENAINLLGLGL"
     gene            14814..15827
                     /locus_tag="VF182_14955"
     CDS             14814..15827
                     /locus_tag="VF182_14955"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438435.1"
                     /translation="MAIWLVAVIVIFGLLDHLGQSYAAAAGKQVVIAHAAMNARVAPL
                     WVARDRGFFAKYGVEADTIFVRGAPTLVAAMQSNEIDVGYTGGTAVVGAVANGADLKV
                     LSAFTNRVTYDLMVKPGIKRPEDLRGKIFGIQSIGGTVWMGAILALEHLKLDAERDKI
                     NIIAVGDQNVLAQALEAGTIDATVLDGVQSRRLNALGFPSLADLNKANLPILSSGFVM
                     REPNLQKNPQLAENLMKAVIEGAAFSLSMSQKPSTLKILQKYLRITEKDAEEGYRDLV
                     SGTDKKPYAAPEGVSNVVRLMKRSNPKVEKVKPEQLIDDRILKKLDQSGFIDAVLSKY
                     GVK"
     gene            16018..17151
                     /locus_tag="VF182_14960"
     CDS             16018..17151
                     /locus_tag="VF182_14960"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438436.1"
                     /translation="MYIDGDTHYWPLRFIDKVSHPGKGHVEITDDTGDYLRYGDVVPG
                     KVATYYRDGKKIHSFKEGRWSIPLRAEFMKKDGFDIQVLIPDNRPLIYECDAELGRQL
                     ARAYNDTVAEDIQGDDRFIGVAWIYLPDIKESIRELRRAVKDLGLKAVKLNGGWGDGD
                     LDNEVLWPLYEEISDLDIPILLHPAARVFELQHSHPWLVGSERFQGYQHFPTALGFSL
                     TYMVSASRLIFGGVLDRFPTLKFAFFEGGIGWVPWLMHTLNAHTPNEAGVSVAVQQFF
                     EGKQAIKKLPSEYFDQFYIAAVSWESYLAETIKGWADHNIIIGSDFDHGDAVATWPRT
                     VAVIKGMKELSEQDKERVLGGNAMRLFSINGKDAAGHAAQVPM"
     gene            17382..18827
                     /locus_tag="VF182_14965"
     CDS             17382..18827
                     /locus_tag="VF182_14965"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6438437.1"
                     /translation="MAKDLRTFIADCQRELPGEVIHIKKEVNPANYDVTAIIKHLGAQ
                     KKFPIIIFDNPLNLNGKVGPIKLTMNCEISQGKTQIALGLPKDASRPKMAEKCLEMEE
                     HRIPPTIVDKKQAPVKENTMVGKDVDLYQIPIMRHHEMDGGPYIVLSTITKDRKSGIY
                     NCSYHRMEVKSKNSTALYASPRHLWKIYKDYEDNNMEMPVATVLGHHPAYHMGACYSG
                     PFEVSEYDVIGGYLGEPLRLVASETFGEDLLIPADAEIVIEGVLKPGKRVVEGPFGEA
                     PGYLGPQRYTTCVDYEVRAINYRNGAMYQSVITPEGDKPWMDLPREGAYLRRCREAVP
                     GVTAVCKQGRHAHYNVFISMKKMSEGDPGRAAAAALTFDHTKNVFVFDEDIDVFNPTD
                     ILWAIATRVQPHRQVNILQPLFRGNFLDPSLVDEIKTSGMIVDATKPLDRPFSPVSKC
                     PDEAMARIKLEEYVPGEVLQHIPMDRTTYWA"
     gene            19069..21372
                     /locus_tag="VF182_14970"
     CDS             19069..21372
                     /locus_tag="VF182_14970"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6438438.1"
                     /translation="MNYTETFSIAGKDAHRVDGIEKVTGKAVYTGDIELAGMGYAKIL
                     RSPVAHARLLKVDASRAKELAGVIATLTRDDIKEFNYKYGATYKDQSIVAVDKVRYVG
                     DPVAAVMAEDPAIAEAALDLIEVEYEELPKVTNIEEAIAPGAIQVHEGDVARAELRGS
                     TYGAPPKFNGTNICYYLGFGRGDVDKAFAQADHVFEDTFRFQKVQHYSLEAHINIASY
                     DGEKLTVWASCQDPFTLRDHLSGIFGLPLSRIRVIVPYVGGGYGGKLYVKAEPIAAAL
                     TWKTRRPVKLALSVNESFKTVTRHPARVTLKTAVSKEGRLVARECQIFMDTGAYADAG
                     PRVTQKAGYRSLGPYKIPHAKVEAHGVYTNTVPAGAFRGFGALQVTWAYESQMDMMAD
                     RLGIDPLEFRLKNLLKKGDLYTAGDTPVDCDMEEGLRKVAETIKWQERDTKPNRGKGL
                     SCCIKDGGGTYKVAGATVKMSSDGSVVLLTGTVEMGQGPRTALSQVVAEELGLSLDQI
                     TVAQLDTDVTPYDISTSASSSMVVMGLAVQRAAQDAKRQLFQCAGKVLKQKPEDLFIQ
                     NGEIHSQNGQSISYSKVIVDFFGSKAGEIIAKGLYKDKRSKKAVLGSTATFWEVGWGG
                     VDLEVDPETGVVRLLNYVSICDTGKAINPDQCIGQDEGAVMFGIGHSLMEEMVYEDGQ
                     LLNGNLVDYRVPTFRDLPEGLHTILLENGNGPGPFGSKGIGEGGLLPVASAIANAVSR
                     AVGVRIQDLPLTPPKIWRALQAKAEVQ"
     gene            complement(21487..21990)
                     /gene="nbaC"
                     /locus_tag="VF182_14975"
     CDS             complement(21487..21990)
                     /gene="nbaC"
                     /locus_tag="VF182_14975"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HEX6438439.1"
                     /translation="MLPHLKGFNLQQWINENRNNWGQRRVIWDDSDFIAFVTRGPNRR
                     KDFHINPGDEIFYQLEGELNLHYLSDGKPELAVLKSGDLFLMPARVPHSPRRREGSWT
                     FVIERKRSREENDLFIWICEECQNRLYQTDIRFDDPSDAVAKASEAMRSDKNLATCKS
                     CGAVLEL"
     gene            22052..22552
                     /locus_tag="VF182_14980"
     CDS             22052..22552
                     /locus_tag="VF182_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017291761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX6438440.1"
                     /translation="MTARKQRRFSLEKGRIGLISDTHGLVRPEALAALKGVELIVHAG
                     DIGKSEVLDRLQSIAPLAVIKGNNDTGPWARHIPEILDVHVKDRTLRVIHNVRDSESN
                     LRARGIDVVISGHSHKPSVETRDNVLFVNPGSAGPRRFKLPVTVGLLLLQEAHPRAEI
                     IHLLSP"
     gene            22747..23295
                     /gene="pyrR"
                     /locus_tag="VF182_14985"
     CDS             22747..23295
                     /gene="pyrR"
                     /locus_tag="VF182_14985"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX6438441.1"
                     /translation="MASEKQIVLTKQELANAITRMAHEVLEKSGSFEELTLIGIRSRG
                     VHVARRLARRIEELTRHPPPVGVIDVTLFRDDKTQTQASAAPTAGEIAVSLENKVAVL
                     VDDVIFRGRTIRAALEALERLGHARRILLAVLIDRGARELPIRADIVGKNIEASDSQR
                     VNVLLEESDGIDQVIVTPWKVA"
     gene            23306..25231
                     /locus_tag="VF182_14990"
     CDS             23306..25231
                     /locus_tag="VF182_14990"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX6438442.1"
                     /translation="MSTANLVSIADLSNDEIEEIFDLADASDKLRSKKVGSGKIMATL
                     FYEPSTRTRLSFESSMQRLGGTVISCSDMQSSSAAKGESVADTAKVVSSYADVLVVRH
                     YWDGAVQAVAEHADVQVINAGDGSHEHPSQTLCDLYTLRKEKGELKGLNVVICGDLKN
                     GRTIHSLVYALARFGANVVTLAANGMELPQYVLERLEREFHYTLAPVASDDLSAVVTE
                     TDALYLTPKQPHQLALFTEVDSTIQTRITGLVSGLRYDAFYMTRKQKERMKNAADGGD
                     YPIIGAKFLQERRFKDTVVMHPLPRVDELSPELDKDRRGIYFKQAAYGVPIRMALLHF
                     LFEKKRRGAAAKPVKNAVFESPHPIGPHCPNVKCVTVNEPGSTRLRFDLLSEGPAGAL
                     ILRCLYCDHRFKVQFVGQTTSKKYCAYDIALADNIGHWLEKRELTIFDSIKQAEELGY
                     EPYKSGPGRAIMDQAEVESAVQQISEQISRDGGDPDRLLIMGVKTNGSIIAQRIAANL
                     ERAQGRKFEVAELETYGAGEDLRRLFPVDPELGPIHLRDRSIILVDDVIHTGRTVKTA
                     LSIIFRSGRPRSVRLAVLIDRGHREIPVKPNYVGKHIPTADHERVRVHLRELEPDGKD
                     KVIVYPIINPTENRSRL"
     gene            25328..26500
                     /gene="carA"
                     /locus_tag="VF182_14995"
     CDS             25328..26500
                     /gene="carA"
                     /locus_tag="VF182_14995"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX6438443.1"
                     /translation="MVRKAILALADGRCFEGWSFGAEGEAAGEVVFNTSMTGYQEILT
                     DPSYEGQLVAMTYPQIGNVGINPEDVESHRPFVKGFIVKEYTAVPSNWRATQPLHVYM
                     QQHGIVGIEGIDTRSLVRHLRDCGAQVGVITTIAQNRDEAIAKAKTAPGLEGRDLVRN
                     VSCASAYEWHQGVWRLGEGYRTRGDEPRSKKIAARGEHFDAPLHSVVAYDFGIKYNIL
                     RNLADSGCEVKVVPAQTSAQDVLALKPDGIFLSNGPGDPDAVSYAKDNVRKLIGKKPI
                     FGICLGHQILGLALGGKTYKLKFGHHGGNQPVMDLTTRKVEITSQNHGFAVDAESLKG
                     SVEVTHLNLNDNTVEGLVHRDLPIFSVQYHPESSPGPHDANYLFRRFIELMDQHHG"
     gene            26510..29749
                     /gene="carB"
                     /locus_tag="VF182_15000"
     CDS             26510..29749
                     /gene="carB"
                     /locus_tag="VF182_15000"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX6438444.1"
                     /translation="MPKRTDIKSILLISSGPIIIGQACEFDYSGTQALKALKEEGYRV
                     ILVNSNPATIMTDPEFAHRTYVEPITTDIVQKVIAKERPDALLPTLGGQTALNLAIDL
                     AEKGILARYGVEMIGAKVESIKKAEDRDLFKEAMKRAGLDLPRSGYARSMRDALRIQK
                     RLDFPVIIRASRTLGGSGGNFAFGPEEFQDVVKAGLSISPVHEVLIEESIIGWKEFEL
                     EVMRDHKDNVVIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRDASLRVIREIG
                     VETGGSNIQFAVNPKDGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     IRNDITQETPACFEPTIDYVVVKIPRFTFEKFPQAKDLLTPQMKSVGEAMAIGRTFQE
                     SLQKAMRSLEIGSFGFEPRVDLAPERRDQALNTVREKLKNPNSQRLWYLADALRLGVP
                     AEEIYQLSGFDPWFVEKVRGIVATEGEIAKNHGKFDEVTATAMRQWKQMGFADPRLAH
                     LAGTTEKEVRRQRKSQGIEAVFCSVDTCGAEFKAFTPYLYSTYEGEDESKPTPRKKIM
                     ILGGGPNRIGQGIEFDYCCVHAAFALREDGYETIMVNCNPETVSTDYDTSDKLYFEPL
                     TLEEVLNIVDRENPDGVIVQFGGQTPLKLALPLEHAGVKIIGTSPDSIDLAEDRERFS
                     ELLKRLGIRQPDNGIARSYEGAFHIAERLGYPVLVRPSYVLGGRAMEIVYDADALRRY
                     MTYAVSASPEHPVLVDKFLDDAIEIDVDALSDGDQVIIGGIMEHIERAGVHSGDSACS
                     LPPKSISAEVQDVIRQHSIALAKALDVRGLMNVQFAVQDKNVFVLEVNPRASRTVPFV
                     SKATGVPLAKIAARVMVGRKLRELGVTTEIVPNHISVKEAVFPFNKFPGVDTLLGPEM
                     KSTGEVMGIDANFGVAFAKAQLGGGMRLPRKGRVFISVRDEDKKTAAAIAQKLQHGGF
                     RIIATRGTADYLKERGIAAEIVNKVQDGSPHIGDKIRTGKIAMVINTPADANSQADSY
                     DLRRSALDFQIPYFTTMAGAEAASEAIEYLRKNDFEVQALQDYLNRPLG"
     gene            29758..31959
                     /gene="recG"
                     /locus_tag="VF182_15005"
     CDS             29758..31959
                     /gene="recG"
                     /locus_tag="VF182_15005"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX6438445.1"
                     /translation="MNLPRRRASKSPSVPGTSSSNQASPRNLQTPLRFLKGIGPRRAE
                     QLAAFGLQTVEDLLYHLPFRYEDRRQVKNIDQAIVGQEETFIGTLAIIQKKYNPRRRS
                     QVLIGRLTDQSGAMGLLWYRAPSYLVNALIPGQRLAVHGRVETGIGGLKQLVHPEFEI
                     LEADNLGKEPKILPVYEHPATVPLSLMRKWLAEALAAYREKLANHLPLSTMERQKLID
                     PTEALVQLHQPPAQANIAALNRFSSPAHRSIIFDELFYLQLGLAIRKKKNRGSRGVPV
                     NGGGSLTRKMAALLPFKLTGAQQRVLGEITENMESAEVMQRLVQGDVGAGKTMIAWFS
                     SLRVIENGFQAVWMAPTELLAEQHFRSIQPYAKQLAISARLVTGSLTAKERNMVLDEI
                     SKGGIQFIVGTHALIQEAVQVPRMALGVIDEQHRFGVVQRLSLQRITGRADTALSLTE
                     QPHMLLMSATPIPRSLAMVLYGDMEISVIDEMPPGRLPVKTQVFTEAQRKTVYLRVME
                     ELRRGHQAFVVYPLVEASEQLQHVRDATQMAEKMRQSVFKEFGVGLIHGRMAGAERDQ
                     VMRAFRDGSIRVLVSTTVIEVGIDIPNATVMVIEHAERFGLSQLHQLRGRVGRGTAAA
                     YCLLVNRAPRNPVAQQRLRVMERESDGFRIAEADLALRGPGEFLGTRQSGLSDFRLAN
                     LARDSRLLIEARAEAQAWLQKDPELKSRESQEMRRILLQRWGQRLQLGAVG"
     gene            32484..33842
                     /locus_tag="VF182_15010"
     CDS             32484..33842
                     /locus_tag="VF182_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010043665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6438446.1"
                     /translation="MVNNLEVSKEPQQSSLKRVIGQRMLLFFVLGDILGAGIYVLVGK
                     VAGHVGGMLWLPFLLAFVLALLTAAAYSELAGKYSQAAGAALYVNKAFGIPFVTFIVA
                     FAVMMSGVTSAGVAAQAFGGRYLAEFVSLPVIVGALIFLGLVTLVNFIGISESVKVNV
                     TLTVIEGSGLLVIIAIGIYALLTGGGEPSRALEFSSSDGAFLAVLQGTALAFYALLGF
                     EDSVNLAEEAKDPARDFPYALFGGVMVAALIYVAVAFTATMLVDTQTLTHSSGPLLEV
                     VKVAGLAFPPKLFALIALFAVANTALMNMIMASRLVSGMARQRVVPSFLASVHSARGT
                     PWVAILFTTAIAVVLVATGDIGGLADTTVLLLLCVFALVNVSVLVLRRDRVGHKHFRA
                     PTWMPVLGAVVCLILALPITGRDTAVYMRVGVLIAIGVVLWFINRLYVGKVKEFDATQ
                     IT"
     gene            34262..35245
                     /locus_tag="VF182_15015"
     CDS             34262..35245
                     /locus_tag="VF182_15015"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438447.1"
                     /translation="MRKPAGIFVLLLLSIIVAPQLGSAETVRAGYTSRDLNYLPFFIA
                     QKHFHPKQGLQVDLVSIGRSDVQLQALVTGDLHFANINADGIIIWNERSGAGGDLKVA
                     AGSSNAAPYLLIGAKNIKRVEDLKGQRLGVASLRGGATSILLSYLKSKGLIYPRDFSM
                     VVIAGGTPARLSALESGAIGGAVLGIPYGDIAVDKGFPSLGDTMEVISTYQFNSININ
                     PAWAEKNRSSVVKFIKGHIQALRWIYDRPSEAADFLTKELGLQAPYARRGIDYYIKKK
                     VYAPNGEVTLPGLKVNIEVQAQDGVLKEPLPSPEKYTDLSYLRQAQKELGL"
     gene            35418..36530
                     /locus_tag="VF182_15020"
     CDS             35418..36530
                     /locus_tag="VF182_15020"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438448.1"
                     /translation="MKELQIIDADSHIEEVTEIWDHLEPAFQSRRPFPVTVEKNIGHT
                     NLNAFWYIDGAAVPKLEGPGTILIATPLTSSYAQAKPFSVGSQGLTDVKARLKDLDDF
                     GLDVQVIYPTLFLATLTDDLGFEAALMRAYNSYLAHACAQAPDRLKWAGVVPLRAPTE
                     AAKEVRRCKELGASAITTMGTAGEKLLHDKSLFPVYAEMERQNLPFCVHVGWSHPGLN
                     QSCENIYMSRISFTLPVLAAFWSILGGGVLDEFPRLRVAFLEAGSEWLPYWVGRMDHY
                     YHADTGNARGGYMPKKRPPEYLKEGRVFFTCEAEEASLPQVLDLLGEDHMMASADIPH
                     GEARERSMDVVRGRSDLSERAKKKILGENAVRFYDL"
     gene            complement(36612..37544)
                     /locus_tag="VF182_15025"
     CDS             complement(36612..37544)
                     /locus_tag="VF182_15025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6438449.1"
                     /translation="MANCLEGAQRAEAYGFDSVWVRDHLVFEPHGIEGSDNTHIEGLL
                     LLSAIAAVTDRISLGTAMAICHRHPIYLAQLFAGLSVISRGRVIMGVGLGGFPHEFAA
                     AGYPTALDQRARLVRSNVQICRRLWTGERVSFADEYFQFADVTLKPCPVRPIPIWYGG
                     GTPAACRRAVEYCDGWMPARTTLATFEGLIAYLRKLCQDANKPMISAAVMPFTSLDRD
                     RKTALSKISIESLLVEANKFPTWVKPQSGVFSTLDDIRGLILAGTPADVIRETRAYEA
                     AGANHIVYDLRFRYADWHEQLDLLGQEVLPALRG"
     gene            37692..39374
                     /gene="ettA"
                     /locus_tag="VF182_15030"
     CDS             37692..39374
                     /gene="ettA"
                     /locus_tag="VF182_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX6438450.1"
                     /translation="MSTEPNKVIYSMVGVSKYYDKKAVLKDIYLSYFYGAKIGVIGLN
                     GSGKSSLLRILAGVDKEFNGEAKLSPGYTVGYLEQEPLLAEARTVREVVEEGVQETVD
                     LLKEFEEISAKFAEEMSDDEMSALLERQGAVQEKLDHQDAWDLDARLEMAMDALRCPP
                     PETPVKVLSGGERRRVALCRLLLQKPDILLLDEPTNHLDAESVAWLEHHLQSYPGTII
                     AVTHDRYFLDNVAGWILELDRGQGIPWQGNYTSWLEQKQERLRRQEKSESERQKTLQR
                     ELEWIRMSPKARRAKGKARINAYDALLEQESQKRREDLELHIPPGPRLGNTVIEAQAV
                     SKAYGDHLLVDDMTFSLSPGGIIGVIGPNGAGKTTLFRMITEQEQPDSGTIRVGETVV
                     LGYVDQSRNLDPEKSIWEEITGGAESLKLGSREMNSRAYVARFNFSGTDQQKKVGMLS
                     GGERNRVHLAKMLKGGANVLLLDEPTNDLDVNTLRALEEALENFAGCAVVISHDRWFL
                     DRIATHILAFEGDSKVLWFDGNYSEYEADRKQRLGNAAEQPHRIKYRPLTRA"
     gene            complement(39595..41052)
                     /locus_tag="VF182_15035"
     CDS             complement(39595..41052)
                     /locus_tag="VF182_15035"
                     /inference="COORDINATES: protein motif:HMM:NF006056.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438451.1"
                     /translation="MSKENRISRRGFLEAIAALGSLGLASCAANERQASRLSAIDRAT
                     GKLPARSEFVVRNAYVVTMDAKLGDIPRGDVHVRNGTLLAIGPNLTAPGAEQIDGSNR
                     IALPGFVDTHFHLWGSFARGIVADGEFDYFPVMSRLGPAMTPDDAYHSARLGIAEAIN
                     SGLTTIHDWSHNIISGAYADADLRAIRDTGIRARFSYGYSRNLQTKPEQTADFADITR
                     VQREWFGSSNDGLTTMGFASRGAGDTPPKVYRAEWEFARSQGLPITQHAGRSLAEIKR
                     FRRIEMLYKDKLLGPDVQLIHTYSASPEERAMIAETKAHVSIAPFTASRLASGLPYLG
                     DLLSKGVQCSLSVDTTTVGGNADMFGIMRLMLQLNHLRSMNVMEVQPRRILELATLDG
                     AKDLGIADRVGSLTPGKRADLILVRTDDLNVAPFFNPALLLVQQAQPFNVDTVVVDGR
                     ILKHKGELVALDTQEIVRKAAESFTAARKRAGGPY"
     gene            41315..42367
                     /locus_tag="VF182_15040"
     CDS             41315..42367
                     /locus_tag="VF182_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438452.1"
                     /translation="MSYPKTFRISCMLASIFGTALIAVVPVFSQMPYYAGKTIAIIRG
                     GGPGGSGEFQSRALMPYLQKYIAGNPTIVMEYMPGAGGRKAANYFYTAKPDGLKIASA
                     GAMIPGPILGLPGSNYDIDKFPYIGSTETGNPYILFTRKAVGLDTLDKVRRASALRIG
                     AHSVGHSVYVTARMFAYMLDLKQPNFVVGFTDPEMDVAMQNNELDARTTSNTDSLLRK
                     DMMDNIHVHATISNPKGRTDPRLPGLPDLDTFAKNDKERNVIELFRAFQVPRWPHHLS
                     PGTPPELVKILREAMVKTFKDPAFQQEFKKLMGREPTPLTGEDVQKVVKELPRDPEVI
                     GFYKKLAEHEPLPPRG"
     gene            42446..>43144
                     /locus_tag="VF182_15045"
     CDS             42446..>43144
                     /locus_tag="VF182_15045"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6438453.1"
                     /translation="MKKLLLTLLLLFGPVTGLHAQAPFYQGKTITFLVGSGSGTAYDI
                     YSRLLAAYIGKHLPGNPTVVVQNMPAAGGLVAANYVYGVAKPDGLTLASINPAHYFNQ
                     LQGNKEVKFDWAKFAWVASMDKSEHMLYMRSDTPYKTMQDVRKAAEPPKCGATGTGTS
                     GHYIPRMLEETLGTKFTIVTGYAGGNEIDLATERNEVVCRSFTTQAYFQREPYHTWRK
                     KNFVRILLQTGRTRD"
BASE COUNT         9539 a        11590 c        12463 g         9552 t
ORIGIN      
        1 ctttccgaga ccggacaggc tgcaaagtgt cgccgaggac tcgaaaagcc caggttgtgc
       61 catggcgcct ggttcctgca aagagactac aggaattgga tggagcatgc agctccttcc
      121 ttccgcccgc tctacgccga accgcatttg gtatccgaga acaaaaacgc cgcgaccgag
      181 taccgcggcg agcgctgcgt cgttaggcgt cagcgcgccc gaagccgccc gcggttccgc
      241 aagtgtcttg gttgtttgct gcgttagggt cggtggggtc tcgtctggct cagcgaagat
      301 catgtctatt ccgacgcttg ctgccccaag cgcgccgatc ttatccacga gctgcgccca
      361 gccggctgcg cggccagggc cagcgaccaa aatgcgcaag acttcgctcg tcgaggctca
      421 cgatcacgac tcgtcccgat gtctgaaacc ggtgcgcaga atggaacagg atatcgtaag
      481 cttgccgatc cagttcggct aggaggaacg gtcgatacag atagagccca gctactagca
      541 atgaggcgat tagtccccac gcaccaagat acccgtaaga gggacggtct ctgtggagcg
      601 gcattaccac acggtggatg ctgcggagag aacaacatcg tcgagttcgc tcctacagtt
      661 tttatgaagt ttccgcggcc cggggcctat ccgcgctggt gcgctcattg ccaggataag
      721 agccgaactt ttgatgaagc cgatatatcg cggagtcgct tgcgaggagc ttgacgtttc
      781 tatttcattc ggtgtaattc cagatctgct acatttctgt tcatacccgt gaagccgata
      841 tcagagaaaa atctaaccgt acgccggtta caacgggcgc gatgggcgcg tttgaaatgc
      901 agccgagacg caaacggttt tcttggcgtg ttagcggcgc cgatgatcgg agttttgttg
      961 ctcgtttgtt tagctcgcaa gcagctgagc gagtacaacg acttgttagc gacgaaactt
     1021 gcgcgacccg tcatgcacag cctgacattg tttttccaac ccagccgagg ctgagaagat
     1081 tatcgagtat tggaagaata caggaatcga acgtcttcaa aaagcaggtg cggttgatag
     1141 aagcagcgcg aaggtacgtc cctgtgacca acggttgatt tggctctaca caccgctgct
     1201 tttatggatt gcaatcgttg ggcatgaatt cggcagtgaa gcttacggag ggaaaaaatc
     1261 atgttgaaac ccaaaacaat cgcgacgctc ttgatcactg ccggtcattg tttgatagcc
     1321 gcaccaaggc tcgacgcggc cgatgctgcg cttaagaagc tccggatcgg ctatccatcg
     1381 catagcgcca gcatgtaccc gatctatgtt accaaggaag ctaagctgtt ggaaaaatac
     1441 gggctagacg gcgagatgat ttacgttcaa ggggtgcaga tggtgcaagt ccatgttgcc
     1501 ggtcaattgg atgtgacgac gacatccggc ttggttacct tgcagtcgtc ggtttccgga
     1561 gcggatctga tactgttggc aaattctatt gacgtgcatc taatgaagct catcacacat
     1621 cccggcatca acgctcccgc agacctcaag gggaaatcta tcgccgtgac acgcttcggc
     1681 tctttgaccg acctgatcgt gcggccgctt ttggtgagct ggggattgga tccgaaaaag
     1741 gacgtcacac ttttgcagat cggcagccag cgcgatattg ccaccgcgat agcgctcaag
     1801 aaagttgacg ccggcgtgct ttcttttccg acgtcgtact acgccgaaag aaccggtctc
     1861 aagacgctct atgactttac tgaatccggc agcgaaatac cgacgaccac tgtggccatc
     1921 agccgagaat acggcaaaaa gaaccgggat tcggtgctga gattcttacg agcgtacctc
     1981 gagggacaca aaaaactaca gaccgaccgc gccttaagca tacgcgcgct ccggcgatac
     2041 ggcggcatcg ccgatgagga aaccctggct tacacttatg atctgttcag cacgaggatc
     2101 gtgcgcaaga tcccgacgat cagcacgcag agcgtggaca atgcgctgaa attactcgct
     2161 gagactatgc ccaaagcaaa agaacgcaaa gccagcgaat tcatcgatac cagttacatg
     2221 gaagagctgg agcgcacggg tttcatcaag tccctatggc cgtgacgggc cggcggcgcg
     2281 ggcatcctag ttggcggatg ttatagtttt gcgattctcc ggcgctggat atagcaaatc
     2341 gtgaatttcc tctgctgaag gtccgaccag accaagggtc tcgcgcgtgg tgtctggtac
     2401 catcggctgt tcattgttca cgacactatc tttcggttca acggccgctt tatcggctgg
     2461 ctgatcttcg gcgaaaataa ccgggaaaac gcttaccagt agagctagga agcacccgcc
     2521 agatagcaac gtagtgcgac gcttttggta cttcgttttc actttctcct cgtcgcgaag
     2581 cagtaggagt tattttttct cagcccggag tttggttctt gctaaagcaa gcgcaatttt
     2641 agtaccagaa ctcggaagag aggtgtccgc tgcgaatgct cgggaataac ggtgagacaa
     2701 tgcattccgg gatttccgag agggtgcctc tcgttttgag ttgacggcac tgtccactca
     2761 gttgacgtac aaaccagacc cgtccagtga gagcgcgcag ccacttaatg aatcttaatc
     2821 gagagcgact ctctcttccc ggtcagcacg agaaagccgc ctgccagcgt ggcttcaggt
     2881 gatctaccgg accgagtgtg ttaggttatg ttggtttgcg gcaagcttcg agagcccacg
     2941 gaatgcctga atcaacttca aatggaggga cttctaacga ctcgaccgtt ctggcggtgc
     3001 agtcagggtc agcagcggtt cgtttgcgcg aggcgggccg attcctggat cagttcgaac
     3061 ccggcgcaga ggtgcttctc ctcggcgcat cccgcggttc cgtcgacgat ctcgcccgcg
     3121 caattgcgaa cgagagaggg gcgacatttg gactgcatcg actctctttc acacagctcg
     3181 cggctcggct ggctgcccta gagcttgctt cccgcgatcg cgcgcccacc aatgctctgg
     3241 ggcacgaagc ggttgcgacc cgggcggcgt tcgaggccgg cagcgaggag gcgatggaat
     3301 atttctcgcc tgtctcacgg acaccgggtt tccccaaggc gttgtcgcgt accttgctcg
     3361 acttaaggct tgctgctgta gagagcggtg acgtggtccg gctgccgcgc agcggaactg
     3421 atctcgccgg cttgctcgat cgggtcgaga gccttctgaa cgaggccggc gctagcgacc
     3481 gggccgcatt gttcgagatc gcgaccgagg cgttgggctc gcctgtgatt ggctggccgc
     3541 cgatgccgct actgctgctc gatgttcctt tccaatctga aattgaaacg cagtttctct
     3601 ggactctgct taagcagtca ccccaggtga tgattaccgt cgccgccggg gatgtggctg
     3661 cgctcactca gctggaaaag cgcggcgcgc gggtacaact tatcgaggag ggtgaagaga
     3721 ccgatctgac ccagcttacg cgttatcttt tttccaagga gccaccgcgg cggcggacac
     3781 gttcgggtga actggtgtgg ttttcggcgc cgggcgaagg acgcgagtgt gtggagatcg
     3841 cgcgccgtat ccttaaagaa gccgatcgcg gcgtccgctt cgacgagatc gcaatcgtta
     3901 tccggcccgc gcagcagtac gccggactgc tcgagcatgc cctgacccgc gccggaattc
     3961 ccgcctattt cgaccgcggt acccggcgtc ccgatcctgc cggtcgtgcc tttctcgcga
     4021 ttcttagttg tgcaaccgat aatttctccg ccaaacgctt tgccgagtat ctttcgttgg
     4081 ctcaagtgcc ctcagtggat gattccggca aggttccggt gcgatccggt gattcgacgt
     4141 gggtggcttc tcgcgatgat atcttcggaa tcctttccga agctgcattc gatcaagccg
     4201 accatggcga tgtcgaagag gatgagggct tgctgacggc cggcagcgtt gatggtgatc
     4261 gcgcggtcat ggccggcacg ctcagagcgc catggaaatg ggaaactctg ctcgtcgagt
     4321 cggctgttat tggtggaagt gagcgctggg cgcggcgctt aaacggtctc gccgcagaat
     4381 acgaattgaa aattcgcgcg ctaacattcg agcaagcaga ttcaccgcgc attgctcacc
     4441 tggagcgcga gcggcagaac cttgcgcatc tgcaaaattt tgccctgccg ctcatcgagg
     4501 ccatgtctgc ctggccgcaa caggctacat ggggagattg gatccagcgc cttgaagaat
     4561 ttgcgccgcg ggtgctgcgg cggcccgagt tcgtgttgcg ggttctggcc gatttgcgtc
     4621 caatgggagc ggtcggtcca gtcactctgg ccgaagtgcg cgcggtgctg gccgatcgcc
     4681 tggcaacact ggaactggag ccacccgcgc atcgctacgg gcgggtattc gtttgtggcg
     4741 ccgatcaagt gcgtggccgc gcttttcggg tggcctttgt gccgggattg gccgagcggc
     4801 tctttccgca aaaactgcat gaagatccgc tgctgctgga cgatcttcga cgtgaaatcg
     4861 gcggcgggct aaccctccag gaggatcgcg ccgagcatga acgactcttg ttgcgcctcg
     4921 ccgtcggcgc tgcggctgaa cgcttgtatg tttcgttccc gcgcatcgaa acgctagacg
     4981 ctcgcgcgcg ggtgccatcg ttttacgcgc tcgaagttat gcgtgctgtc accggccgga
     5041 ttccggatca tcatctgctc gaatctgaag cttcagaaga agccaatgcg agcctcggat
     5101 ggccggcgcc tcagcgcccg gaagacgcca ttgacgactt cgagcatgat ctttcggtgc
     5161 tgcgcctatt gatgcgcagc gacaaggatg tgaagggtca tgctcattat atgcttcgcc
     5221 tcaatgactg cgtccggcgg tcggccagcg agcgttgggc gcgcgcgcgc ccgccatggt
     5281 ccttctatga tgggctcgtc cgaatcacag acgccaccaa gccatttctc caatcgcaac
     5341 ggttgacggt acggccttac tcggtctcgg cgctgcagaa ttacgcctac tgtccttatc
     5401 gattcgtatt gtcggcgatg taccgcttgg aaccactcga ggaacctgag ccgcttgagc
     5461 gtatggatcc tctgactaag ggaaggctgt tccacgaggt gctggcggaa ttctttcgcg
     5521 ccctacagca gagccaaatg acactcccgg cgtcgccgat ggccgcgctg ctcgaactct
     5581 tggacgtcac tcttacacgg gtcgcggcaa attatgcaga gctgctggcg ccggcggtgg
     5641 agcgagtatg gcaagacgag attgcgagca ttcgcaccga tttgcatatc tgggctcgcg
     5701 atctgactca aagcagcgga tgggagccgt ggctattcga gtttggcttc ggcctgcctg
     5761 atgctcccgg ccgagatccg aatagccgtc gggagccggt taaaattgat gggcgattca
     5821 tcctgcgcgg tgcaattgac ttgatcgagc gcaagccggg gacgaacctt ctgaaaatca
     5881 ccgatcataa aaccggcaaa aatcgctctg agcgcggatc tttaatcggc catggccagc
     5941 agcttcaacc ggtgatctat agcttggcag tcgaggcggc aaccgggctc accgtggaga
     6001 gcgcgcgctt ttcatactgc acgactgcgg gtggattcag cgagcatact gttgccatca
     6061 atgaaccgac ccgcaggatg ggtatcgaag cgctggagat catcgaccga gccgtagagt
     6121 ttgctatgct gccgcccgcg ccggccgaga aggcgtgcgc ctattgcgat tttctatccg
     6181 tatgcggtcc gaaccaggag cgccgcgcgc gccggaaatc acgcaaagag atcgccgatc
     6241 tgatcgagct gagaggaaaa caatgacgct gccgccgctc gctgatcaag gcgatcgcga
     6301 tcagattgcg acagcgctgg acgacacgct gattgtcgaa gccgccgccg gcaccggcaa
     6361 aaccaccgag ctggtgcggc gcatcgtgcg ggtgatccaa acgggccgcg ctgaagtgac
     6421 cgggatcgtc gcggttactt tcacagaaaa agccgccggc gagcttaagc tgcgcttgcg
     6481 cgaagcgctc gaagaggcaa gaggatcatt tggcaacgac tccgaagagc gccgccgcct
     6541 cgatacggcg gtgggcagac tcgaagaagc gcaaatcagc accatccacg gattctgtgc
     6601 cgaccttctc agggagcgtc ccatagaagc gcggatcgat ccgctgttta gagtcctcac
     6661 tgaggcgcag gcggtgagta tgtatgatga aggattccac cgctgggtcg agatcgagct
     6721 cgctgatcca tcggcaggcg tccagcgctc tctgcgccgt ccggtgttcg gcggtttcgg
     6781 tgctcaagcc gatggcggtg acggccccgt cgatcggctg cggcgagcag gatgggagct
     6841 gattcaatgg cgcgatttcg acggtgactg gcaacgaccc atattcgacc gcttagggca
     6901 catcgaccaa ttggttcagc tcctgaatga gtttaccgaa ttgctcgaac cgcccggcga
     6961 catccgtgac gcgttgtacg ccgacacgtg ggcggcgagg aagcttgcag aagagatccg
     7021 gcggaccgaa tcggtgtctg cgcgcgatta cgaccggctc gaagcaggtt tcatcgagct
     7081 cagccgcaat cgtaacttta aagatgcgcg aaaagggagc ggcaaaaatt accgcagcgg
     7141 cgtcctacgc gaagatgttt gtcgggcgtg tgaaaacctg aaccacgttc tgggcgattt
     7201 caaagaagag gccgatgccg atcttgccgc ggcgctgcgc gacgagctgc gcggctgcgt
     7261 cagcgcttat gaagcggcca aagccaaagc cggcgccttg gactttctcg atcttctgct
     7321 caaggcgcga gatctcattc gggataacgc ggaggtgcgt tccacctttc aagcgcgttt
     7381 caggcgcatt ttcgtggacg aatttcagga taccgatccg ctgcaggctg agatcttgtt
     7441 gcttctggct gccgatgatg cggctgcgac aaactggcgt gagactcgcc cggttgccgg
     7501 caagctcttt ctggtcggtg atccgaagca gtctatctat cgcttccgcc gcgccgatgt
     7561 cggaatttat cgcagcgtct acgagatgct tcaggcggcg ggcgccagaa gggtgacgtt
     7621 gcggaccagt tttcgcgcgc gtccgaatat gcagcgcgcc ataaacgccg ccttcgagcc
     7681 ggtgatgaac ggcgacggcg acgtgctgca gccggcttac gtgccgctgg agccgtttcg
     7741 tcccgaattt ccagatcagc catcgatggt cgtgctcccc gtgcctgagc cgtacgggta
     7801 ccggagactt tccaatgtct cgatcgagaa gtcgcttccc gatgcggtgg gcggttttat
     7861 cgactggctg attaacgaca gcggctggaa agtcgcggaa cggccagtca cggcaaaatc
     7921 gtccctggcg ccaacggcgg cgcgcggttt ccggcccggc gagaccgagc atcttgtgcc
     7981 gatccagccg cgccatatct gtctgctgtt tcgccgtttc accagcttcg gcgaggacat
     8041 gacgcgtcct tatgtcgaag ctctcgaagc gcgcgggatt gcccatctgc tggtcggggg
     8101 ccgctcgttt cacaatcgcg cggagatcga gaccttgcgc gccgcgctgg ctgccatcga
     8161 gtggcctgat gatgaattat cggtgtttgc aacgttgcgc ggatcactgt ttgcggtcgg
     8221 cgaggaggac ctgctggaat accgccatcg catcggccgg ttccatccgt ttcgcatccc
     8281 caacgaaatt ccggcaaacc ttggggccat cgtccatggt ctcaagtcac tgcaagcgct
     8341 tcatcgccgg cgcaatcggg tgccagtggc gacgacgatt gccgcgttgc tcgaagccac
     8401 gcgcgcgcac gtcggcttcg cgctggagca tggcggcgaa caggtgcttg cgaatgttgc
     8461 gcatgtggtc gatttggcgc gccgctacga ggccgagggg gggatctcgt ttcgtggctt
     8521 catcgaggct ctgcgcgagc aggccgagaa cggcgaagcc ggggaagcgc ccatcttaga
     8581 ggaaggcagt gacggtgtgc gattgatgac ggtgcataaa gccaaagggt tggaattccc
     8641 ggtggtgatc cttgccgaca tgactgcgaa actgcgtgcg tccgccgcca gccggtatct
     8701 cgattcggag cgcaatgttt gcgcgatccg tctcggcggg ttcgcgccgg ccgatttgct
     8761 cgaccacgaa gacgaagaat tgctgcgcga tgaggccgag gggatccggg tggcgtatgt
     8821 cgcggcaact cgcgcgcggg acctgctggt gattccggcc atcggcgatg aggagagaga
     8881 aggctggatc cagccgctca acacagcgat ttatccggcc aaggaagcgc ggcgtgagca
     8941 gattcaagca ccgggatgtg tggaattcca atccaaagat tcggtatggg tacggccggg
     9001 cggcagcgcg gcgagcgcga gcacggtaag ccctggcctg cacgtctgca ggagactagg
     9061 gctttcacag atcgcggagt tctcggtggt ctggtgggat ccgcgcgcgc tcaagctcgg
     9121 cgtcgaagcg ccgcttggtc tgcgccggcc agagctgatc gtgaaagatg tcgagccggc
     9181 gatcgtcgaa tcaggtcttg ccgcttacaa ttcgtggcgc gagcggcgcc agcaagccat
     9241 tgccgccggc agtcgtagtt cggttgcact gcagactgtc acccaagcgg caacgtccgg
     9301 caggcaacca gcgccggatt tgaagcttgc gccggtggaa gtgatcgagt tgccacgcgc
     9361 agccaatcgg ccgtcgggac ggcgtttcgg caccctggta cacgccgttc ttgcgagcgt
     9421 cccactcgat ggcgatgtga gcatcattca acgtcttgca accattcacg gccgcatcct
     9481 cggagcatct caggacgaag tcgatgccgc cggtgagagt gtgcgggttg cgctggcgca
     9541 tggaatcttc gaggacgcgc gccaagctgc tcaacaaggc cgttgtcgcc gtgaagtgcc
     9601 catcacctgg cgcgattatg aagggttgct gattgagggt gtcatcgatc ttgcctttga
     9661 gcgggccggg gcctggacgg tgatcgattt caagactgac gaagagtttc gcggggatga
     9721 acccgcatat cggcggcaag tcggaatgta cgcagcggcc atcaatgccg ccaacggagc
     9781 gccagtcgcg gcgctgctaa tgcgagtgta gcaattgccg ggagcccacg cgaatcgatt
     9841 caattagctg cttgagatgt ggccgatcga ggtgctttta ttttcttagt caacaaatgt
     9901 cgccgctttc tactactgcg gtgaccgggt ccaagtcgtg ccattctgcc aatcgatgcg
     9961 gtctcgtctc acatcgcctc tcaatccacc ttcccagtcg agcgcgcgga tatcgccatc
    10021 tcgagcggtt tgtattcggc tcgtctggcc ccgttcgttt ctcgcttgaa gcccgtcccg
    10081 cgatgagaca atttcaaccc gtttgtcgcg tgcgccgttt agataccagg tcccggctaa
    10141 atcgcggcga tgagacggcg cgcgtgtcca catcgaacca ttcgcccatt cgatcgtgtc
    10201 gcgtttaatg tgcccacgaa ggccaccttc ccagtccaaa gcccggacat tatttccgtg
    10261 cgcttccagt cgactcgttg cgccatgttc gtttctcgct tgaagacccc ggcgggccgt
    10321 gcggatctcc acaggcttat cgcgctcgcc atttgagtac catctgccga gaagacttgc
    10381 ctcacgcctg tccggtcctc ctttgtcgtg cggttgtgcc gatgccgcag tgggagccgc
    10441 tagaagcgct gcgagcaaac cggcataaac cagattaaat tttttcatga tagacctcct
    10501 tcagagataa gccgcataga cgcttttcct tggagaacat cggccgactt cgtttggcgg
    10561 tagagaagtc gtgaacggat aatcgcggct tccctggttg cacggaaaaa ttgatttgcg
    10621 ggtttgaaat ggcgctcatt catgctccgc ccggcttggc ttggtaactc cgatgagtgg
    10681 tgagccggat ggatcagcga tggattacgt gtgccgtacc gaagtcaaca gcagtaccac
    10741 caaataacga gtccgattgt gccatggtcg ttccgaaagt gattgcccac cgtatcatcg
    10801 gagttacccc ccaggctcta ggcgcaagga aaatgccaca gtaaaatccg ccgtgctttg
    10861 gcgtccgttt gattttgagg aagcgcaatg ggatacccct tgcattcctg gggctacgaa
    10921 taggtacatc agggataaaa ctccaacaaa attgccaggc aaaaaccttc tggacaatgg
    10981 atatcgaaca gcagtaaaac tcggataagt cgacccgtcg ttcaaatcgg cgccgcccga
    11041 ttgggctcgc cgctgcgcta ttttcagcgg cgcctcagcc tggctccgag gtagccgaga
    11101 ctgaggtagc cgagactatt gagggaaact gcgcatcttt atgtgatgcc gaatttttat
    11161 ttcaatgatc tcgcgcagcg caagattttg atgcatgcga gaagacgtat ggccaagctg
    11221 acgtttttac tgggattatg cgggtcgggt aagacctaca ttgccgaacg gttagcgacg
    11281 cagaccggag cggatctctt cagcgatttt ctgcaggatc agagtaagaa ccttcctttg
    11341 ctcttaaaaa atctcagcag cggaatcgac tgcattatag atgagtggaa atattctatt
    11401 cccaggttcc gtgataagat cctggtggat ctggtcaatg tacctaatct ccaggtggag
    11461 tggatttgct tcgagaatga tcgggccaac gcggattgga acgtcgttca ccgtaccaac
    11521 aaagcggatg ccgagggaca tctcaggatc aacgagtttc tgtctgatgt atatcagtac
    11581 cctgcgcagg ctcagatttt aaaaattacg cgcattgatg cggggtgaaa ccgtctatcc
    11641 ggtacgcgat cgcttggcgg ccctgtgctt tcctctcggg ataatgataa ataatatatc
    11701 atcaatccta cagtcatgac ccacgaggca tgccatggac gaattaaccg cgttgagcat
    11761 cttagccaat accattgatt cttgtctgaa taatgatctg cccgcggctg aagtatttgc
    11821 ggcgcttaat tttctcgcag cacgtcttcc ggtgaagtgg cctttcgtgg aatttcgcca
    11881 aggcttggac agcggggatg aaactattcg tcatcagatc cttaagtctg cgctggccgc
    11941 gatcaagtta ctcctcaggg ctcgacaacg atagaagagc cggcacctcg tgagtgcggc
    12001 tccactcgcc ggcgttgccg gagcattttc gcgcataaag cgctaggaga ccctaatgga
    12061 aagttgtaaa tgtacagcga ggggacacgg ccaccgcaac aatcaatgcg ataatgccgc
    12121 cgccaccgcc gatggctact gccaaacgtg ctccgagaaa atcggcaagg tggcgcagga
    12181 atcggaacaa tccgggccca agtcacccat gggtcagtag ctttacttat tatggcggcg
    12241 ctctgccggc actccatctt ccatcatctc gttagatgtt gattcaggat cccaggctgg
    12301 cgctgaggcg tcctcggatc gagaaaagaa cttcttacag gttgattgct gatgaacata
    12361 ctcgatgagg tccacattat tggtctccat cgttctaata ttccagttat cgcggccggt
    12421 tcctcccgat atgccgttta caaaaccttc gattgactaa tcgcccttgt ctctgttatt
    12481 ccggcgacag attccgcggc tgagattttt cgccgggttg ccggctttga tcttattcga
    12541 cagttctatt tggagggtgg tcaccgacat gtggcgtagt ttgactcgac tctttgcgat
    12601 cgttgctttc tttgtgatcc aaccggacac cgtcttccct caaacccggc ccaaagttgt
    12661 cgtcggctac gcatcgatga gtacggtagc gacgactctt tgggtggcgc aggagagggg
    12721 attttttgcc aaaaacggta tcgacgtgca gccaattttt atacctggat cgccgacttt
    12781 gatcgcttcg ctcagcaccg gcgacgtgca ttttggatac accggcggca ccgcgacatt
    12841 gggagcggcg gtgggcggtc tggacatcaa aatgatcgcc gcctttgcca atcacgtgca
    12901 gaccgatctg gtggtgcggc cggagatcag aacgcccgcc gatttgcgcg gcaaacggat
    12961 cggcgtcacc agcatcggcg gcaccggctg gatgtcggcg atgctcggcc tcgaacaaat
    13021 cggcttgaat ccggatcggg ataatatctt gctggctgct ttcggcgatc agcgggtgat
    13081 cacccaggcg ctagagacgg gaacgattca gggagcgtcg ctggccggcg ttttcggtca
    13141 acgcttgaag cgagcgggtt accatttcat gggggaactg gaaaaaattc ccctggtcgg
    13201 caccagcatc gtggttaaat cggactatct ggctaagcag caatcgattg tgcgtaatac
    13261 tctaaaggcg ctgatcgaaa gccatgcttt cgtccacaat ccgtcgaaca aggcggcggt
    13321 tatcgaaatc acctcgaaaa agctcggcat cgccgatccg atcgcggcca gtgacggcta
    13381 cgacgattac gtgcggcgca ccgaccgtaa accttttatc gcgatcgagg ggctcaaaaa
    13441 catccagcgt ttcatgaaat tgcgcaaccc caagatagct gaggtcaacc tcgggcggtt
    13501 gatcgacgag agtctattac gcgagctcga caaaagcggt tttgcagaac aatcgctcgg
    13561 cggcacaacg actcgctgac gaataaatat ctcatgggac ctgttcgcaa aggaggaagc
    13621 aattatggcg attgatggaa tgaaagtgat cgatctcgac agccatctgg tgggtgattt
    13681 ggagagttgg gaccagacaa tcgaggcaaa atacaaagat ttcttgccac gcaaactgcc
    13741 gaccagagac aatgaaaggc gcaagactct cgtcggcaat caaatcatga tcggctccga
    13801 gctcggccgc cagaaagcgg agaaaaaaga gtgggtcacg ccgaacgatc tgacggcgca
    13861 cgggcgggtt cgcaacatgg atcttgacgg catcgacgtc gccgtgcttt cgcccaattc
    13921 gccggcgctc gatattctgt ggtttgtcga tgatccggag ctcgcggctg cttacgcacg
    13981 ggcgcaaaat aattacatga actattatgc gtcgcagcaa gcgggccggc tgatgtgggc
    14041 cggcgtcatt cctcttcagg attccaagga agcgatcaag gaattgcacc ggtcgcggga
    14101 aatgggcagc aaagcgctca acgtaaaagc gacgcccatt cctggcaaag agtggtcgga
    14161 tccgcacttc gatccaatct tcgacgaact agaaaaaacc aacactccga ttattttcca
    14221 cgataccaag accggttcca tgggccacga acgcttcgcc gataattttt tcttttcgca
    14281 catggtcggc cgcaccatcg aaagcatggt gtgcttgatg atctatcttt gcggcggcgt
    14341 gatggaaaag cacccgaatc tgaagatcat ttgcctcgaa accggcgctt cccagatgcc
    14401 ctggtggttg tcgcgcatgg atgagcattg ggagaagctg ccgcacttgg ttccttggca
    14461 aaagcgcaag cccagcgata ttttcaatga gagtgtctat gtcggttgcg agccgttcga
    14521 agacgggttg tttgaatggg cggttgaata tctcggcggc gaccggcttg tgttggcaac
    14581 cgattctcct cattgggatt cgtcacagcc cggccaggtg accggtcccg tggccaagag
    14641 cacgaagatt tctcacgata acaagcgcaa ggttttgggc gaaaacgcta tcaatctctt
    14701 ggggctcggt ctctaactgc attggccgag cgctcaggag cgataccaac cgggcgaggc
    14761 cgaacaatca ataaaaacaa gatcaaattc ctatgccgaa gaaaggagat tgcatggcta
    14821 tctggttagt cgctgttatt gtgatcttcg gtttacttga ccatttaggc caatcgtatg
    14881 ccgctgcggc aggcaagcag gtggttatcg ctcatgcggc gatgaacgct cgagtcgcgc
    14941 ccctctgggt tgcccgcgat cgcggctttt ttgccaaata cggcgtcgaa gccgatacga
    15001 tctttgtccg cggcgcgccc actctggtcg cagccatgca gtcaaacgag attgacgtcg
    15061 gctacaccgg cggcaccgcg gtggtcggcg cagtcgccaa tggcgccgat ctaaaggttc
    15121 tttcagcgtt cactaatcgc gtcacctacg atttgatggt caagccggga atcaaacggc
    15181 cggaggattt acgcggtaag atattcggca ttcaaagtat cggcggcacc gtttggatgg
    15241 gagcgattct ggccttggag cacttaaaat tggacgccga gcgtgacaag atcaatatca
    15301 tcgctgttgg cgatcagaat gtgctggcgc aagcgctgga agccggcact atcgacgcga
    15361 cggtgctgga cggagtgcag agccgccggc tcaatgccct gggcttccca agccttgccg
    15421 atttaaataa agcgaatctg ccgatcctaa gctccggctt cgtcatgcgc gaacccaatt
    15481 tgcaaaaaaa cccgcagctg gcggaaaatc tgatgaaggc ggtcatcgaa ggcgctgcat
    15541 tttctttatc gatgtcgcag aagcccagca cactaaagat tttgcagaag tatctccgta
    15601 tcacggaaaa ggacgccgaa gaaggatatc gagatctggt ttcgggcacg gacaagaaac
    15661 cctatgctgc gcccgagggt gtcagcaatg ttgttcgatt gatgaaaaga agcaatccga
    15721 aggttgaaaa agtaaaaccg gaacaactga tcgatgatcg gattttgaaa aagctcgatc
    15781 agagcgggtt tattgacgcg gtgctgtcca agtacggagt gaagtaagcg agcagctgaa
    15841 aacgtctcta atctgcgtcg tcgcgcttgt tcgctctcac atcgtgctcg tgactgcgtg
    15901 tccgtgtttg tttgccattc gcgatcatgt tttcacacac acgatcacga ggattctccg
    15961 cttcgcatca ggaatcttgc gacctgctca aatccagcca gaggagggat tgaatcagtg
    16021 tacatcgacg gcgatactca ttactggcca ttacgattta tcgacaaagt ttctcacccg
    16081 ggaaaagggc acgtcgaaat caccgatgat accggcgatt atcttcgtta cggcgatgtg
    16141 gtgccgggca aggtcgcaac ttattatcgc gacggtaaga aaattcactc gttcaaagaa
    16201 gggcgctgga gcattccgct gcgcgctgag ttcatgaaaa aggacgggtt cgatatccag
    16261 gttttgattc cagataaccg gccattgatc tatgaatgcg atgccgagct tggccgccag
    16321 ttggcgcggg cgtataacga cacggttgcc gaagatattc aaggcgatga ccggttcatc
    16381 ggcgtcgcct ggatttattt gcctgacatc aaagaatcga taagagagct gcgccgcgca
    16441 gtgaaagatc tcggccttaa ggcagtaaag cttaatggcg gttggggtga cggcgatctc
    16501 gacaatgagg tcttgtggcc gctctacgaa gagatctcgg atctcgatat accgattcta
    16561 cttcatccag cggcgcgggt ctttgagttg cagcatagcc acccctggct cgtcggcagc
    16621 gaacgctttc agggatatca acacttcccg acggcgctgg ggttttcgtt gacctatatg
    16681 gttagcgctt cgcggttgat attcggcgga gtactggatc gcttcccgac attgaaattc
    16741 gcttttttcg aaggcggcat cggctgggta ccttggttga tgcatactct caacgctcat
    16801 acgccgaatg aggcgggtgt ctcggtcgcg gtgcaacaat ttttcgaagg aaaacaggcg
    16861 atcaaaaagc ttcccagcga atatttcgat caattctaca tcgctgcggt atcgtgggaa
    16921 tcctacctcg ctgaaacgat caaaggttgg gccgatcaca acatcattat cggcagcgat
    16981 tttgatcacg gcgacgccgt tgccacttgg ccaaggacgg ttgcagtgat caaagggatg
    17041 aaagagttat cggagcagga caaggaacgg gttctcggtg gcaatgcgat gcgcttgttt
    17101 agcatcaatg gtaaggatgc ggcggggcat gcggctcagg ttcctatgta gaacttcagt
    17161 ctgtcagtac ggctctttgc aggtgaacca gccgtctgtt gtgtgtcggt tcaactgctg
    17221 acatatcgtc gaacttcgga tctggtggcg cgctgattag gctcgaagag cactggcgcg
    17281 tgagtctgga acactcctca tgggcggttg aattgacaat gatgactgca ttggataagc
    17341 attttgcata aaatctttca tacattcgct aggaggagtc gatggcaaag gatcttagga
    17401 cctttattgc tgattgccag cgtgagttgc cgggtgaagt gattcacatc aagaaagaag
    17461 tcaatcctgc aaactacgat gtgacggcca tcatcaaaca tctcggtgcg caaaagaaat
    17521 ttcccattat cattttcgac aatccgttaa atctcaacgg taaggtcggt ccgattaagc
    17581 ttacgatgaa ctgcgaaatt tctcagggaa aaacgcagat agccttgggc ttgcctaagg
    17641 atgcaagccg acccaagatg gctgagaaat gtctcgagat ggaagagcat cgaattcctc
    17701 ctactatcgt cgacaagaaa caggctccag taaaagaaaa tactatggtc ggtaaggatg
    17761 tcgatcttta ccagataccg atcatgaggc accacgagat ggatggaggg ccttatattg
    17821 ttttgtcgac gatcactaag gacaggaaat cagggatcta taattgctcg tatcaccgca
    17881 tggaggtgaa atccaagaac tccacggctt tgtacgcgtc gccgcgccac ttatggaaaa
    17941 tttacaagga ctacgaggat aacaatatgg agatgccggt cgcgaccgtg ctcgggcatc
    18001 atccggcgta tcacatgggc gcatgctaca gcggaccgtt cgaagtcagc gagtatgacg
    18061 tgatcggcgg ctatctcggc gaacccttgc gattggtcgc ttcggaaact ttcggcgagg
    18121 atttgctcat cccagctgat gctgaaattg tcatcgaagg cgtgttgaaa cctggcaagc
    18181 gagttgtcga aggccccttc ggggaggctc cgggttatct gggtccgcag cgttatacga
    18241 cttgcgtcga ctatgaagtc cgcgccatca attaccgtaa cggcgcgatg taccagtcgg
    18301 tcatcacacc ggaaggggac aagccgtgga tggacttgcc gcgcgaaggc gcctacctgc
    18361 gtcgctgccg cgaagccgtt cccggcgtga ccgcggtctg taaacagggc cggcacgcgc
    18421 attacaatgt atttatttcg atgaagaaaa tgtccgaagg cgatccgggt cgcgccgccg
    18481 ctgccgcatt gacgttcgat cataccaaga atgtgttcgt gttcgacgag gatatcgatg
    18541 tgttcaatcc caccgatatt ctctgggcga tcgcgacacg cgtgcaaccg catcggcagg
    18601 tgaacatttt gcagccgttg ttccgcggca attttcttga tccatcctta gttgatgaga
    18661 tcaaaacttc gggtatgatc gtcgatgcca cgaagcccct ggaccgacct ttttcaccgg
    18721 tatccaaatg tcccgatgaa gccatggctc gcatcaagct ggaggaatac gtgcctggag
    18781 aagtcttgca gcacattccg atggatcgga ccacttactg ggcataaatc ggtacctgag
    18841 aattaattta taaagccagc ttcaggagga atcatgggcc aggcggttga agttacgtgt
    18901 ccggcgtgta agaaaacttt tgttgttaat ccgcatatgc tgggatccgg tatgaacttc
    18961 cattgtccgt actgcgatct ttattttccg gaaaaagata gtccaaaaat ccgcaagtga
    19021 tgttgaggcc gcggtgtatg aacaccgtcg cctctacaaa ttccgatcat gaactataca
    19081 gaaacctttt ctatcgccgg caaagacgcg caccgcgtcg atggtatcga gaaagtgacc
    19141 ggcaaggccg tttacaccgg agacattgag cttgcgggta tgggttacgc caagatactt
    19201 cgcagcccgg tggcccatgc ccgtttgctc aaggtcgatg cgtctagagc gaaagaactg
    19261 gccggagtca tcgccactct gacccgcgac gatattaaag agtttaatta caaatacggc
    19321 gcaacttata aagatcagtc gattgtcgcc gtggacaagg tccgttatgt cggcgatccg
    19381 gttgccgcgg tcatggccga agacccggcg attgccgaag ctgccttgga tcttatcgaa
    19441 gtagagtacg aagagttgcc gaaagtaacc aacattgagg aagcgatcgc gccgggagcc
    19501 attcaggtgc acgaaggcga tgtggcgcgc gcggagctgc gcggctcgac ttatggggcg
    19561 ccgccgaaat tcaacggcac caacatttgt tattatttgg gcttcggcag gggcgacgtc
    19621 gataaagctt tcgcgcaggc cgatcacgtg ttcgaggaca cgtttcgttt tcaaaaggta
    19681 cagcattatt ctcttgaggc gcacatcaat attgccagct atgacggcga aaagctgacg
    19741 gtatgggcct cgtgccagga tcctttcacg ctgcgtgatc atttgtcggg aatcttcggc
    19801 ctgccgctca gccgaattag agtcatcgtg ccctacgtgg gcggcggata cggcggaaaa
    19861 ttgtatgtca aagccgaacc gattgccgca gccttgacct ggaagacccg gcgaccggta
    19921 aagctggcgc tgtcagtcaa cgaaagcttc aagaccgtca cgcggcatcc ggctcgggtc
    19981 acgctcaaaa ccgcggttag caaagagggg cgcttggtgg cgcgagagtg tcagattttt
    20041 atggataccg gcgcttacgc cgacgcgggg ccgcgagtca cacaaaaagc cggttatcgt
    20101 tcgctcggtc cttacaagat tccccacgcc aaagttgaag cgcacggagt ttataccaac
    20161 accgttcccg caggggcttt ccgtgggttc ggcgcattgc aggttacctg ggcgtatgaa
    20221 tcgcaaatgg atatgatggc cgaccggctg gggatcgatc cgctcgagtt tcgcttgaag
    20281 aacctgctca agaagggcga tctttatacg gccggcgata ccccggtgga ttgcgatatg
    20341 gaggaggggc tgcgcaaggt ggccgagact atcaaatggc aggagcgcga caccaaaccc
    20401 aatcgaggca aaggccttag ctgctgcatc aaagatgggg gcggcactta taaggttgcg
    20461 ggcgccacgg tcaaaatgtc gtcggacggc agcgtcgtgc tattaaccgg aaccgtcgag
    20521 atgggacaag gtccgcgcac cgcgttaagc caggtcgtcg ctgaggagct cggcctcagc
    20581 ctggatcaaa tcacggttgc ccaactcgat accgatgtga ctccctatga catatcgacg
    20641 agcgcgagca gctcgatggt ggtgatgggc ctggcggtgc agcgcgccgc gcaggatgct
    20701 aagcgccagc tttttcaatg cgccggcaag gttcttaaac agaaacctga agatctgttc
    20761 attcagaatg gcgagatcca ctcgcaaaac ggtcaaagca tcagctatag caaggtcatc
    20821 gtcgatttct tcggcagcaa agctggagag atcattgcca aagggctgta taaagacaaa
    20881 cgaagcaaaa aggcggtctt gggctcgacc gccacttttt gggaagtagg ttggggtggt
    20941 gttgatctcg aagtagatcc ggaaaccggc gtcgttcggt tgctcaatta cgtttcgatc
    21001 tgcgatacag gcaaggcgat caatcccgac cagtgtattg gccaggacga aggcgccgta
    21061 atgttcggca tcggccactc gcttatggag gaaatggtct acgaagacgg ccaattgttg
    21121 aatggcaatc tggtcgatta tcgggtgccg accttccgcg atttgccgga aggcttgcac
    21181 acaatcctgc ttgagaatgg caatggccct gggccgttcg ggtccaaggg gattggcgaa
    21241 ggcgggttgc tgcctgtcgc ctcggcgatt gccaatgcag tttcgcgcgc ggtgggggtg
    21301 agaattcagg atttgccatt gacgccgcca aagatctggc gcgccctgca agctaaagcg
    21361 gaagttcaat gaggatcaaa gcgaaagctc gtttacagac ctgttctaca tatctttgag
    21421 atagtatttt gacattatta tcagattctt agacagtttt tttgtagctt aggttaaagg
    21481 cacaaattac agctcaagga ccgcaccgca gcttttgcag gtcgccaggt ttttatcgct
    21541 gcgcatcgcc tcgcttgctt tcgccactgc gtctgaagga tcatcgaaac ggatatcggt
    21601 ctggtagagc ctgttctggc attcttcgca aatccagata aaaagatcgt tttcttcccg
    21661 agagcgtttc ctttcaatca caaacgtcca cgaaccttcc cgtcgccgcg gcgagtgggg
    21721 tactcgcgcg ggcataagaa aaagatcgcc gctcttgagt accgcgagct ccggcttgcc
    21781 gtcggataga taatggagat tgagttcgcc ttcgagttgg tagaagattt catcaccggg
    21841 gttgatatga aagtctttgc gccggttggg accgcgggtc acaaaggcga tgaaatccga
    21901 atcgtcccag atcacccggc gttgtcccca gttgttgcgg ttttcgttga tccattgctg
    21961 taagttaaaa cctttcaggt ggggcaacat acaacctcct tggctgcagt cgaatatagg
    22021 attgagtgca acgaggaatc aagagcgctc attgacagca cgcaaacagc ggcgcttctc
    22081 gttagagaaa ggacgcatcg gtttaatttc tgacacccac ggtttggttc gaccggaagc
    22141 cctcgcggcg ttaaaaggcg tcgagctcat cgtgcatgcc ggcgatatcg gcaagtctga
    22201 ggtgttggat cgcttgcaat ccattgcgcc gttggcagtc atcaagggca acaacgatac
    22261 ggggccgtgg gcgcggcaca ttcccgaaat tttggatgta cacgtcaaag atagaactct
    22321 acgggtgatt cacaatgtcc gggatagcga atcgaatctc cgcgccagag gcatcgacgt
    22381 cgtcatcagc gggcattcgc ataagccgtc ggtcgaaacc cgagacaatg tgctttttgt
    22441 aaaccccgga agcgcgggtc cgcggcgttt caaactgccg gtgacggtag ggttgttact
    22501 attgcaagaa gctcacccgc gcgcagaaat cattcacttg ctgtcacctt gaagtgtcgt
    22561 atcggaaccc gtctctctcc cacgcgcaat ttcatcgttg actttgatcc tccgtcaggc
    22621 gataaatagc aaatcctttt aaaccgaccc tgtgagatcg gtaagggaat catgcagata
    22681 gttgtgcctc taaaattttt ggttctttgg acctcctcac ccggttgagg gggtcttttt
    22741 ttgtctatgg cgagcgaaaa acaaatcgtc ctgaccaagc aagaattggc caacgcgatc
    22801 actcgcatgg ctcacgaggt gctcgagaaa tccggcagct ttgaagagtt gaccctcatc
    22861 ggcatacgtt ctcgcggtgt gcatgtcgct cgacgtttgg cgcgtaggat cgaagagctt
    22921 acccggcatc caccgcctgt cggtgtcatc gatgtgacgc tttttcgtga tgataagacc
    22981 caaacacagg cttcggctgc gccaaccgcg ggtgaaatcg cggtatcgtt ggaaaataag
    23041 gtcgccgtct tagtcgatga tgttattttt cgcgggcgaa caatccgcgc ggccctggaa
    23101 gcgctcgagc gactcggcca cgccaggcgg attctgctgg cggttctaat cgatcgcggc
    23161 gcgcgggagt tgccgatccg ggccgatatc gtcggcaaga atatcgaagc ttcggattcg
    23221 caacgagtga acgtcttgct cgaagaatcc gacggcatcg atcaagtgat tgtcacgccc
    23281 tggaaggtag cttgaggaat cggacgtgag cacggctaac ctagtttcca tcgcggatct
    23341 cagcaatgac gagatcgaag aaatcttcga tttggcagac gcctcggaca agctgcgcag
    23401 taaaaaggtc ggcagcggca agatcatggc gacgttgttc tacgaaccca gtacgcgcac
    23461 acgtttatca ttcgaatctt cgatgcagcg gcttggcggc accgtgatca gctgttcgga
    23521 tatgcagtct tcgtcggcgg cgaaggggga gagcgtcgcc gatacggcca aagtagtttc
    23581 aagctatgcc gatgttttgg tcgtgcggca ctactgggac ggcgcagtcc aggcagtcgc
    23641 tgagcatgcc gatgttcagg tcattaacgc cggcgacggc agccatgaac atccgagcca
    23701 aacgctttgc gatctgtata cgctgcgcaa ggagaagggc gagctcaaag gtctcaacgt
    23761 ggttatctgc ggcgatttaa agaatggccg gacgattcat tctctggtgt atgcactggc
    23821 gcgattcggc gccaatgttg tcactctggc ggccaacgga atggagctgc cacaatatgt
    23881 tctggagcga ctcgagcgcg agtttcatta cactctggcg ccggttgcga gcgatgatct
    23941 gagcgccgta gttaccgaga ccgatgctct atatctcact cccaagcagc cgcatcagct
    24001 ggctttgttt actgaagtgg acagcacaat tcagacgcgg attacaggtc tggtgtcggg
    24061 attgcgctat gacgcttttt acatgaccag aaagcaaaaa gagcgcatga aaaacgctgc
    24121 agacggagga gattatccaa tcatcggcgc taaatttctc caagagcggc gcttcaaaga
    24181 caccgtcgtg atgcacccgt tgccgcgggt cgacgagctt tctcccgaac ttgacaagga
    24241 ccggcgcggt atctatttca agcaagccgc atacggcgtg cctattcgca tggcgttgct
    24301 acattttttg tttgaaaaga agcgccgtgg agcggctgct aagccggtga aaaatgcggt
    24361 gttcgaaagt ccgcacccca tcggcccgca ttgccctaac gtcaagtgcg tcaccgtcaa
    24421 tgagccgggt tcgacccggc ttcgttttga ccttctgtcg gaaggtcccg ccggcgcatt
    24481 gattctgcga tgtctttact gtgaccaccg cttcaaagtt cagttcgtcg gtcaaacgac
    24541 gtcgaagaag tattgcgcct atgatatcgc gcttgcggat aacatcggtc attggcttga
    24601 gaagcgcgaa ctgaccattt ttgattcgat caaacaagcc gaagagctcg gctacgaacc
    24661 gtataagagc gggccgggtc gtgccatcat ggatcaagct gaagtagaat ctgcggtgca
    24721 gcagatcagc gaacagattt cgcgggacgg cggcgatccg gatcgcttgc tgattatggg
    24781 cgttaaaacc aacggatcga tcatcgcgca gcgaatcgca gccaatctgg aacgagcaca
    24841 agggcgcaag ttcgaagtcg ccgagctcga aacctatggc gcgggcgaag accttcggcg
    24901 actgtttccc gtcgatcccg agctgggacc catccacttg cgggatcgct ccattatcct
    24961 cgtcgatgac gtcattcaca ccggacgaac ggtgaaaact gccttgagca tcatttttag
    25021 atccgggcga cctcgatctg tgcgtctggc ggtcttgatc gatcgcggac atcgggagat
    25081 tccggtgaag cccaattacg tcggtaaaca tatacccact gcagatcacg agcgcgtgcg
    25141 ggttcatttg cgtgaacttg agcccgacgg gaaagataag gtgattgttt atccgatcat
    25201 taacccaacc gagaaccgga gccgattata aattgcgcga atttctatta tgctgagtgt
    25261 tcaattggtg attgtgattt atcattcttt acatgaggtt ttcagcaaga gctagaacgg
    25321 gaatttgatg gtaagaaaag caatattggc cctggccgat ggccgctgtt tcgagggctg
    25381 gtcttttggc gccgaaggtg aagctgcggg ggaagtcgtg tttaacacca gcatgaccgg
    25441 ttatcaggag attctcaccg acccgtcata cgaaggtcag ctcgtcgcca tgacttatcc
    25501 gcagatcggc aacgtcggca tcaacccgga ggacgtagaa tcccacaggc cttttgtcaa
    25561 agggttcatc gtcaaagagt acactgcggt gccgagtaat tggcgcgcga cgcagccgct
    25621 gcacgtttat atgcaacagc acggcattgt cggcatcgaa gggatcgata cacgctctct
    25681 ggtccgtcac ctccgcgatt gtggcgcgca ggtgggcgtg atcacaacga ttgctcagaa
    25741 cagggacgag gcgattgcaa aggccaagac cgctcccggg cttgaaggcc gcgatctcgt
    25801 gcgcaacgtg agctgcgcga gcgcctatga atggcatcaa ggtgtgtggc ggctgggcga
    25861 aggatatcgc acgcggggag acgagccccg gtcaaaaaaa atcgccgcgc gcggcgaaca
    25921 ttttgacgct cctctgcatt ctgtcgtggc ttacgatttc ggcatcaagt acaacatctt
    25981 gcgaaacctc gctgacagcg gatgcgaggt caaagtcgtt ccagcccaaa cctccgcaca
    26041 agatgttttg gcgttgaaac ctgacggcat atttctgtcc aatggtccgg gtgacccgga
    26101 cgcggtgtct tacgctaaag acaacgtccg caagttgatc ggcaagaaac caattttcgg
    26161 tatctgcctg ggccatcaaa ttttgggttt ggccctgggt ggcaagacct ataaactcaa
    26221 attcggtcac catggcggca accagccggt gatggacttg accacgcgca aagtagagat
    26281 cacctcgcag aaccacggct tcgcggtcga tgccgagtcc ttgaagggtt cggtcgaagt
    26341 tacccatctc aatctcaacg acaatacggt tgagggactg gttcatcggg atctgccgat
    26401 cttctcggtg caataccatc cggagtcgtc acccggaccg cacgacgcca actatctttt
    26461 ccgacgcttc atcgaattga tggaccaaca tcacggttaa tatccctcca tgccgaaacg
    26521 caccgatatc aaatcgatcc tgctgataag ctcaggtccc atcataatcg gccaggcttg
    26581 cgagttcgat tattccggta cccaggcgct aaaagcgctc aaagaagaag gttatcgggt
    26641 catcctcgtc aactccaatc cggctaccat catgaccgac ccggagtttg cacatcgaac
    26701 ctacgtcgag ccgatcacca ccgatatcgt ccagaaagtg attgccaagg aacggccgga
    26761 tgcgcttctg cccaccttgg gcggacaaac ggcgctcaac ttggcgatcg atctggcgga
    26821 gaaggggatc ttggcgcgtt atggggtcga gatgatcggg gccaaagtgg aatcgatcaa
    26881 gaaggccgaa gatcgggatc tgttcaagga agcgatgaaa cgggccggtt tggatctgcc
    26941 gcgcagcggc tatgcgcgca gcatgcgcga tgcccttcgc atccagaaac gcctcgattt
    27001 tcctgtcatc atccgcgcct cgcgcaccct tggcggcagc ggcggcaatt tcgcctttgg
    27061 cccggaggag tttcaagacg tcgtgaaagc gggcttgagc atctcaccgg tgcacgaagt
    27121 tttgatcgaa gagtcgatta tcggctggaa agagttcgag ctcgaagtca tgcgcgatca
    27181 caaggataat gtggtgatca tctgctcgat cgaaaatttc gatcccatgg gtgtgcatac
    27241 aggagattcc atcacggtgg cgcctgccca gaccctgacc gacaaagagt atcagattat
    27301 gcgcgatgcc tcattgcgcg tgatccgaga gatcggcgtc gaaactggcg gttccaatat
    27361 tcagtttgcg gtcaatccga aagatggccg catggtggtc atcgagatga atccgcgggt
    27421 ttcgcgcagt tcggcattgg ccagcaaggc aaccggtttt cccatcgcta aaatcgccgc
    27481 gaagctggca gtgggctaca cgttagatga gatccgtaac gacattaccc aggaaacccc
    27541 ggcttgcttc gaaccgacta tcgattatgt cgtggtcaag attccacgct tcacgtttga
    27601 gaagtttcct caagccaaag atttgctaac accacaaatg aagtcggtgg gcgaagccat
    27661 ggcgattggc cgcacctttc aagagtcgct gcagaaggcc atgcgttctc tcgaaatcgg
    27721 gtcattcggc ttcgagcccc gggtcgatct ggcgcctgag cgacgtgatc aggccttgaa
    27781 caccgttcgt gagaaactga aaaatcccaa ttcgcaacgg ctctggtatt tagctgacgc
    27841 tctccggctc ggcgtgccgg ccgaagagat ttatcagctc tccggttttg atccatggtt
    27901 cgtggaaaaa gtgcgcggaa tcgtcgctac cgagggtgag atcgcaaaaa atcacggcaa
    27961 gttcgatgaa gtcaccgcga cagcgatgcg gcaatggaaa caaatgggct tcgccgatcc
    28021 gcggctagcc catctagccg gaacgaccga aaaagaggtg cgccgccaac gaaagtctca
    28081 gggcattgag gcggtatttt gctctgtgga tacctgcggc gccgaattca aggcgtttac
    28141 tccgtatctc tattcgactt acgaagggga agatgagtca aagccgaccc cacgtaaaaa
    28201 aatcatgatt ctcggcggcg gccccaatcg aatcggccag ggtatcgagt tcgattattg
    28261 ttgcgtgcat gccgcctttg ctctcagaga agacggttat gagaccatca tggtcaattg
    28321 caatccggag actgtcagca cggattacga cacctccgat aagctctact tcgagcccct
    28381 caccctggaa gaggtgctga acatcgtcga tcgagaaaat cccgacggtg tgatcgttca
    28441 gttcggaggg caaacgccgc ttaaattggc gttgccgctc gaacacgccg gagtaaaaat
    28501 aatcggcacg tcacccgaca gcattgatct cgccgaggac cgcgagcgct tttccgagct
    28561 gctcaaacgg cttggaatca gacaacccga taacggcatt gcgcgctcct atgaaggagc
    28621 ctttcatatc gccgagcgct tgggatatcc ggtgttggtt cgtccctcgt acgtgctcgg
    28681 cggccgggca atggagatcg tctatgatgc ggacgcctta agacgttata tgacctatgc
    28741 ggtgtcggca tcgccggagc atccggtgct ggtggataag tttctcgatg atgccataga
    28801 aatcgatgtc gatgcgttgt cggacgggga tcaggtcatt atcggcggga tcatggaaca
    28861 tatcgaacgc gccggtgtgc actccggcga cagcgcgtgc agtttgccgc ccaaatcgat
    28921 cagcgcggag gttcaagacg ttatccggca acatagcata gcgctggcca aagctctcga
    28981 cgtccgcggc ttgatgaacg ttcagttcgc cgttcaagac aaaaacgtct tcgtgctcga
    29041 ggtcaatccg cgcgcttcgc gcactgttcc ttttgtcagc aaagctaccg gtgtgcctct
    29101 ggcaaaaatc gccgcgcgcg tgatggttgg ccgaaagttg cgcgaactcg gcgtcaccac
    29161 ggagatcgtg ccaaatcata tttcggttaa ggaggcggtc tttccgttta acaaatttcc
    29221 cggggtcgat actttgctcg ggccggagat gaaatctacc ggagaagtga tgggaatcga
    29281 tgccaatttc ggagttgcgt ttgcgaaagc tcaattaggc ggcggtatgc ggttgccgcg
    29341 taagggacgg gtgtttatca gcgtccgtga cgaagacaaa aagaccgccg ccgccatcgc
    29401 gcagaaatta cagcacggcg gatttcgaat catcgcaacc cggggcaccg cggattactt
    29461 gaaagagcgc ggcatagccg ccgaaatcgt caacaaagtt caagacggca gcccgcatat
    29521 cggtgacaag atcagaacag ggaagatcgc catggtgatc aatacgccgg ctgatgcgaa
    29581 ttcccaagcc gattcgtatg atcttcggcg tagcgcgttg gattttcaaa ttccttactt
    29641 taccaccatg gctggcgccg aggctgccag cgaggctatc gagtatctgc gcaagaacga
    29701 cttcgaagtt caagcattac aggattatct caatcggccg ctggggtaat cgccttcatg
    29761 aacctgccgc gccgccgggc ctccaaatca ccttctgtcc cggggacttc ttcatcgaac
    29821 caagcttccc cccgcaacct tcaaactccg ctgcgttttc tcaaaggtat cggtcccagg
    29881 cgcgccgaac agttggccgc attcgggctg caaacggttg aagatctttt gtatcatctg
    29941 ccgtttcgct acgaagaccg gcgccaggtt aaaaacatcg atcaagccat agttggccaa
    30001 gaagaaacat ttatcggcac gctcgcgatc attcaaaaaa aatacaatcc gcgcaggcgc
    30061 tcacaagttt tgattggtcg attaacggac cagagcggcg ccatgggtct gctctggtat
    30121 cgcgcgcctt catacctggt caatgctttg atccccggac agcgtctggc cgttcatggc
    30181 agagtcgaaa ccggcatcgg tggattgaaa cagctcgtcc atccagaatt cgagatcctc
    30241 gaagccgaca atttagggaa agagccaaag attcttcctg tgtacgagca tcccgcgacg
    30301 gtgccgctct cgttgatgcg caagtggctt gccgaagcgc tggccgcgta tcgagaaaag
    30361 ctcgcaaatc atttgccgct gtcgaccatg gaacgacaaa aattgatcga tccaaccgag
    30421 gccctggttc aactccatca gccgccggcg caggcaaaca ttgcggcact caatcgcttt
    30481 tcctcaccgg cgcatcgctc gatcatcttc gatgagttgt tttatcttca actcggtctg
    30541 gcaatcagga agaaaaaaaa tcgcggcagc cgcggcgttc ccgtcaacgg cggcggctct
    30601 ttgacccgaa aaatggcagc gctcttgccg tttaagctga ccggcgcgca gcagcgagtc
    30661 ttgggcgaga tcactgaaaa tatggaatcg gccgaggtga tgcagcgttt ggttcaaggg
    30721 gacgtcggcg cgggcaagac tatgatcgcg tggtttagtt cattgcgcgt gatcgaaaac
    30781 ggttttcaag cggtctggat ggcccccacc gagctgttag ccgaacaaca tttccgcagt
    30841 atccaacctt acgcgaagca acttgcaatc agcgcgaggc tggtgaccgg gtcgctgacc
    30901 gccaaagaac gcaatatggt tctcgacgag atctcgaagg gaggtattca gtttatcgtc
    30961 ggtacccacg cgctgattca agaagccgtg caggtgccgc gcatggcttt gggtgtcatc
    31021 gacgaacagc atcgtttcgg ggtggttcaa cgtctttcgc ttcaacgcat tacgggccgc
    31081 gccgataccg cgctttctct cacggaacag ccgcacatgc tgctcatgag cgcgacgccc
    31141 attccgcgca gtctggcgat ggtgctctac ggcgatatgg agatctcggt gatcgacgaa
    31201 atgccgcccg gacgcctgcc cgtcaaaacc caggtgttta ccgaggcaca acgaaaaaca
    31261 gtttatttac gggttatgga agaattgcgc cgcggacatc aagcttttgt tgtctatccg
    31321 ctggtggaag cgtccgaaca actacagcat gttcgcgatg ccacgcagat ggccgagaaa
    31381 atgcgccagt cggtgttcaa agagttcggt gtcgggttga tacatggccg catggctggc
    31441 gccgagcggg atcaggtgat gcgcgccttt cgtgatggaa gcattcgcgt tctggtatcg
    31501 accaccgtca tcgaggtggg tatcgacatt ccgaacgcga cggtaatggt catcgagcac
    31561 gccgagcggt tcggcttgtc gcaactgcac caactccgtg gccgggttgg gcgaggcacc
    31621 gccgcggcct actgtttgct ggttaaccgc gcgccgagaa atccggtggc gcagcaacgc
    31681 ctgcgcgtga tggaacgcga gtcggacggt tttcgcatcg ccgaagccga tttggcgctg
    31741 cgcggcccgg gcgaattttt gggcacacga caatccggtc tgagcgattt ccggctcgcc
    31801 aatctggcgc gcgatagccg cttgctcatc gaggcgcggg ccgaagcgca ggcgtggctg
    31861 caaaaggatc cagagctcaa gagccgtgag tcccaggaga tgcgtcggat tctgctgcag
    31921 cggtgggggc agcgcttaca actgggcgcc gtgggctaag actcttgtag cgcgatccgg
    31981 gcgcaaggct ctggatagca tcatactttc cttataaagc aacgacttgg tctgtcccga
    32041 gctaggctaa atacgccgta agcgcgttcc ggcaagcaat gcatggacgg gcggcgacgc
    32101 cgccgacaaa gatcgctaca gcttcgatgc caccccatac gtttgccgcg caagcgatgg
    32161 taagcgcggc cacaaagatt ccacagtgaa atcttgctgg attctgtgat tccagcgact
    32221 agttccgcgg tgattcattc gatcgctcat cctctcttag cttatccggg aatatattcg
    32281 gccaagccgg ttcgagcaat gacggatccg aattctcggg attcgcagta tctttcttga
    32341 agaagctcgg gtaagaggct agcgttgttg tagatacgga tcgatcttct gaatgattgc
    32401 cggtcggttc aaccttgagt tagggttcct gcagagcggc gggtcgaccg ctaaaagaag
    32461 atgagccttg atagtgccag cgcatggtta acaacctgga agtatcgaaa gagccgcagc
    32521 aatcctccct caaacgggta atcgggcaga ggatgctgct cttcttcgtg ctgggcgata
    32581 tcctgggcgc cggaatctac gtgctggtgg gcaaagtggc gggccacgtc gggggtatgc
    32641 tgtggctgcc atttcttctc gcgttcgtgc tcgcgctctt gaccgcagcc gcatattccg
    32701 agctcgccgg taagtattct caagctgcgg gcgccgcgct ctatgtcaat aaagcattcg
    32761 gcattccgtt tgtgacgttt atcgttgcat tcgccgtgat gatgagcggt gtgacttccg
    32821 ccggagtggc ggctcaggcg tttggaggca gatatctcgc agagttcgta agcctgccgg
    32881 tgattgtcgg agcgttgata tttctcggtc tcgtgaccct ggtaaatttt atcggtattt
    32941 ccgagtccgt caaagtgaat gtgacgctca cggtgatcga aggatccggg ttgctcgtga
    33001 ttatcgcgat cggcatttat gcattgctga ccggcggagg tgagccgagc cgagcactag
    33061 agttcagctc cagcgacggc gcctttctcg ctgtgctgca agggacggcg ctggcgtttt
    33121 atgcgctgct tggcttcgag gactcggtta atcttgccga agaggcgaag gacccggcga
    33181 gagattttcc ttatgcgctg tttggtggcg ttatggtcgc ggcgttgatt tacgtggcgg
    33241 ttgcgtttac ggccacgatg ctggtggaca ctcagaccct gacgcattct tccgggccgc
    33301 tgcttgaagt ggtcaaggtc gcaggcctgg cttttccgcc caagctgttc gcgttgatcg
    33361 cgctgtttgc cgttgccaac accgctctca tgaatatgat catggcgtcg cgtttggttt
    33421 ccggcatggc tcgccagcgg gtcgtgccat cttttttggc cagcgtgcac tcggcccgcg
    33481 gcacgccgtg ggtggcgatt ctgtttacca ctgccatcgc tgtggtgctg gtggctaccg
    33541 gagatatcgg cgggctcgcc gataccactg tgcttttgct gctgtgcgtc tttgcgctgg
    33601 tcaacgtatc cgtactggtg ctccggcggg atcgcgtcgg tcataagcat tttcgcgcgc
    33661 cgacatggat gccggttctg ggcgcggtgg tttgcctgat cctggcactg ccgattaccg
    33721 gccgtgacac ggcagtctac atgcgagtgg gtgttctgat tgcgatcggc gtcgtgctat
    33781 ggttcatcaa tcgcctgtac gtcggcaagg tgaaggagtt cgatgctacg cagatcacgt
    33841 aaccgttcgg tgacgattag gtctccataa catcattgtg taggtgccga actcagtcga
    33901 tccatcaatt cggaatggat ctcgcccaag acgccgttcc tgctgcccga gagcgaggtc
    33961 gtgaaacctc ttctgacacg ttgtcgctat ggtaagcacc aactctgtga tccgaagtaa
    34021 atctggacgt tctcgagtaa atcggttttt acccttgtcc catcgtttac tgcctgctgc
    34081 agttccgcca taaatgcgtg ctcgcgaaga cgccggccag tcatacgacc tgcaaagcca
    34141 aatttaaacg gtcgtttcca ttggtatctc gatccctaac acgggagttt cttttgacgc
    34201 cgagtcggag ccttgataaa accgccgaag atcgccccag gaaaaggaga gtctttgcgc
    34261 tatgagaaag ccagcgggta tttttgtctt gctcctccta tcgattatcg ttgctcctca
    34321 gctcggcagc gccgagacgg ttcgcgccgg atatacctct cgggatctta actatttgcc
    34381 gtttttcatc gctcagaagc actttcatcc taagcaagga ttgcaagtcg atcttgtcag
    34441 catcggccgg agcgatgttc agcttcaggc gctggtcacc ggtgatcttc acttcgctaa
    34501 tattaatgcc gacggcatta tcatctggaa cgagcgcagc ggcgctggcg gcgatctcaa
    34561 ggttgctgca ggctcgagca acgcggcgcc ttatctttta atcggagcaa aaaacatcaa
    34621 gcgggtcgag gatctcaagg gacagagact cggcgttgcg agcttgcgcg gcggcgccac
    34681 ttctattctg ctgtcctacc tcaagagcaa agggcttatt tatccgcgcg acttttcgat
    34741 ggtggtgatc gcgggcggca cgccggcacg gcttagcgct cttgaaagcg gcgccattgg
    34801 gggcgccgtc ttgggcattc catatgggga tattgcggtc gacaaagggt tcccgagcct
    34861 gggcgacacg atggaggtga tctcgactta tcaattcaac tccatcaata taaatccggc
    34921 gtgggccgaa aaaaatcgct cctcggtcgt caaattcatc aagggccaca tacaggcttt
    34981 gcgctggatt tatgaccggc cgtccgaagc cgcggacttc ttgaccaaag aactcgggct
    35041 acaggcgcct tacgcgcgcc gcggcatcga ttattatatt aagaaaaaag tctacgcccc
    35101 taatggcgag gtgaccttac ccggactcaa agtgaacatc gaggtgcagg cgcaagacgg
    35161 tgtcttgaaa gagccgctgc cgtcgccgga aaaatatacc gatttaagtt atctgcgcca
    35221 ggcgcaaaaa gagctaggac tgtaaaaatt ttccgagtga tgatgataag gtgacgaaca
    35281 ttgttgtcga tcctctcatc gaggaggtaa tgatttagga gatcatcgga gagcaggtta
    35341 tggaaaaatt taaaatcatc gatgccgatt ctcatatcga ggaagtcatg gtttaggaga
    35401 tcatcggaga agtgattatg aaagagttac aaattatcga tgccgattct catatcgagg
    35461 aagtgactga gatctgggat catctcgagc cggcgttcca atcgcggcga ccgtttcccg
    35521 ttactgtcga aaagaacatc ggccacacga acctgaacgc cttctggtac atcgacggag
    35581 ctgcggtgcc caaactagag ggacccggta caatcctgat tgcgacgccg ctaacttcaa
    35641 gctatgcgca agccaagccg ttcagcgtcg gcagccaggg acttaccgac gttaaggcgc
    35701 gtttgaagga tctcgacgat tttggcctcg acgttcaggt tatttatcca accttgtttc
    35761 tcgccacctt gaccgatgac ctcggcttcg aagctgcgct gatgcgcgcg tacaacagct
    35821 acctcgccca cgcctgcgcg caggcgccgg accgcttgaa atgggcgggt gtcgtgccgc
    35881 tgcgcgcgcc gactgaggca gccaaagaag tacggcgttg caaagaactc ggtgcctccg
    35941 cgatcacgac gatgggaacc gccggtgaga aactcttgca cgataaatcc ttgttcccgg
    36001 tttacgcgga aatggaacgg cagaacctgc cgttctgtgt tcacgtcggc tggagccatc
    36061 cggggcttaa ccagagctgc gagaatatct atatgtcgag gatctcattc acgcttccgg
    36121 tgctcgcggc cttttggtcc attctcggcg gcggcgtgtt ggatgaattc ccccgcttgc
    36181 gtgttgcctt tcttgaggcg ggttcggagt ggctgccgta ctgggtcggc cggatggatc
    36241 actactatca tgccgatacc ggcaatgcgc ggggcgggta catgccgaaa aagcggccgc
    36301 cggaatactt aaaagaaggg cgggtgttct tcacctgcga agccgaggag gcgtcattgc
    36361 cgcaggtgct cgacctgctc ggcgaggacc acatgatggc ttccgcggac attccgcacg
    36421 gcgaagcgcg cgagcgatcc atggacgtcg tgcgcggccg gagcgatctt tccgaaaggg
    36481 caaagaaaaa gatcctcggc gaaaatgcgg tgagattcta cgatctgtaa tccggtttca
    36541 tccgtttacg tattcgtggc atccgcgatg cagctaacgg cgggtgaccg cgacggagcc
    36601 tgtggttttc ttcagcctct taaagccggc aagacttctt gccccaataa gtctagttgc
    36661 tcgtgccaat cggcgtagcg gaaacggaga tcgtaaacga tgtgattggc gccggcggct
    36721 tcataggcgc gcgtttcgcg tatcacatct gccggcgttc ccgccaggat caagccgcga
    36781 atgtcatcga gtgtcgaaaa cacgccgctc tgcggtttaa cccaggttgg aaacttgttg
    36841 gcctccacga gcaatgactc gatgctgatc ttgctgagcg cggtcttgcg atcgcgatcg
    36901 aggctggtaa acggcattac tgccgcgctg atcattggtt tgttcgcgtc ctgacagagt
    36961 tttcgcaggt aagcgataag tccttcgaaa gtcgccaggg tggtccgggc cggcatccag
    37021 ccgtcgcagt attcgaccgc gcgccggcag gcggccggcg tgccaccgcc gtaccagatt
    37081 ggaatggggc ggaccggaca tggcttcagc gtcacgtcag caaactgaaa atattcatcc
    37141 gcaaaagaca ccctttcacc agtccataag cgccggcaaa tttgcacgtt gcttctgacc
    37201 aatctggccc gttggtcgag agcggttgga taacctgccg cggcaaattc gtgcgggaat
    37261 ccgcccaagc ccactcccat gatgacccga ccgcgcgaaa tcacgctcaa gccggcgaag
    37321 agttgcgcta gatagatcgg gtggcgatgg caaatcgcca tcgcagttcc cagtgatatg
    37381 cggtctgtta ccgcggcaat cgccgaaagc agcaacaatc cttcgatgtg agtattatcg
    37441 ctgccctcga tgccatgcgg ttcgaatacc aggtgatccc tcacccaaac agaatcgaag
    37501 ccataggctt ccgcccgctg cgcgccttcg aggcaatttg ccaccgaggc gtgctcgcaa
    37561 aaatgaggca acagaaggcc gaatttcagc gtcatccgca taacgaagtg tcagtcaatt
    37621 ggatatttct tgcacaatac agcctggcag gctagcttga agaacccaat ttcaagagaa
    37681 atgatttaga tatgagcacg gaacccaaca aggtcatcta ttcgatggtc ggcgtcagta
    37741 agtattacga caagaaggcc gtcctaaaag atatttatct ctcctatttt tacggcgcca
    37801 agatcggcgt catcggtctc aacggatccg ggaaatcctc gttgctgcgc atactcgctg
    37861 gagtcgacaa ggagttcaac ggcgaggcca aactctcgcc gggttataca gtcggctatt
    37921 tggagcagga accattgcta gccgaggctc gtaccgtccg tgaagttgtc gaagaaggcg
    37981 tccaagagac cgttgatctt ttgaaagagt tcgaagagat cagcgccaaa ttcgccgaag
    38041 agatgtccga cgatgaaatg agcgcgctgc tcgagcgcca aggcgcggtg caggaaaagc
    38101 tcgatcatca ggacgcatgg gatctcgatg cgcggttgga gatggccatg gatgcgctgc
    38161 gctgcccgcc tccagaaacg ccggtcaagg tgttgtcggg cggcgagcgt cgccgcgtcg
    38221 ctctgtgccg cttgctgctg cagaagcccg atattctttt actcgatgag cccaccaatc
    38281 acctggatgc cgagtcagtg gcgtggctcg agcatcatct tcagagttat cccggaacca
    38341 tcatcgcggt gacccacgac cggtactttc tcgacaatgt cgcaggatgg attctcgagc
    38401 tcgatcgcgg ccaaggcata ccatggcagg ggaattacac atcgtggttg gagcagaagc
    38461 aggagcggct gcgccggcag gaaaaatccg aaagcgaaag gcagaagaca ctgcaacgcg
    38521 agctcgaatg gattcgcatg tcgcctaagg cgcggcgggc caagggcaaa gcgcgcatca
    38581 atgcctatga cgcgctgctt gaacaggagt cacaaaagcg ccgcgaagat ctggagcttc
    38641 acatcccgcc cgggccgcgt ctcggcaaca ccgtgatcga agcgcaagcg gtttccaaag
    38701 cctatggcga tcacctgctg gtagacgata tgaccttttc actgtctccc ggcggcatca
    38761 tcggcgtcat cggtccgaac ggcgccggca agacgacgtt gtttcggatg attacagaac
    38821 aggagcaacc cgacagcgga accattcgcg tcggtgagac ggtcgtcctc ggatatgtcg
    38881 atcagagccg gaacctcgat ccggaaaaat ccatctggga agaaatcacc ggcggcgccg
    38941 aatcgttgaa actcggctcg cgcgaaatga actcgcgcgc atacgtggcc cggtttaatt
    39001 tctccgggac cgatcaacaa aagaaagttg ggatgctatc aggcggcgaa cgtaatcgag
    39061 ttcatctggc aaagatgttg aaaggcggcg ccaacgtttt gttgctcgac gagccgacta
    39121 atgatttaga tgtgaatacg ttacgcgctc tcgaggaggc gttggagaat ttcgccggct
    39181 gcgccgtggt catctcccac gatcgctggt ttctcgatcg gatcgcaacc catattctgg
    39241 cgttcgaagg cgatagcaaa gtgctttggt tcgatggcaa ctattcggag tacgaggccg
    39301 atcgcaagca acggttgggg aacgccgcgg agcaaccgca tcgcatcaag tatcggccgc
    39361 tgacgcgagc ctgaagcggg ctgttgaaaa aggcccacct gcttcgttgc cgcttgttgg
    39421 tctcgcttca acgtactgaa gcatacgcct cagctcatcg aacttcgcgc gcctcgcatc
    39481 tggaccgttt tgaacagccg gcaaactttt agggagcaac gtttaggttc gacgagatcc
    39541 gttaactcca atctcactgt ttgggcaccc tgttcaatga tctttccgca caatctaata
    39601 cggcccgccg gctcgttttc gcgctgcggt aaacgactcc gccgccttgc ggactatttc
    39661 ttgagtatcc aatgcgacca actctccctt atgtttgaga atgcgcccat cgaccaccac
    39721 cgtatcgacg ttaaatggtt gcgcttgttg caccagcagc agggcgggat tgaaaaaagg
    39781 cgcgacattg aggtcgtccg ttcgcaccag gatcagatcg gctcgtttgc cgggagtcag
    39841 agagccgacc cgatcggcta tgccgagatc tttggccccg tcaagagtgg ccagctctaa
    39901 aatccgccgc ggctgcactt ccatgacatt catcgagcgc agatggttga gctgcagcat
    39961 gagccgcatg atgccgaaca tatccgcatt accgccgacc gtggtggtgt ccaccgagag
    40021 actgcattga actcccttac taaggagatc gccgaggtag gggagaccgg aagcgaggcg
    40081 cgacgcggtg aaaggcgcaa tactgacgtg ggctttggtt tcggcaatca tggcacgttc
    40141 ttcaggcgag gcgctgtagg tgtgaatcag ctgcacatcg ggacccaaca gcttgtcctt
    40201 atacagcatt tcgatgcggc gaaatctttt aatctccgca agtgagcgtc cggcgtgctg
    40261 cgtaatcggc agtccctggc tgcgagcgaa ctcccactcc gcccggtaaa ccttgggcgg
    40321 cgtgtcgccg gcgccgcgcg atgcaaatcc catggtggtc aacccgtcgt tcgatgatcc
    40381 gaaccattcg cgctggaccc gcgtaatatc ggcaaagtcg gcggtctgct ccggcttggt
    40441 ctgcaggtta cggctgtagc cgtaagaaaa gcgcgcgcgg atgccggtgt cgcgaattgc
    40501 ccgtagatcg gcgtcggcat aagcgccgga aattatattg tgcgaccagt cgtgaatcgt
    40561 ggtcagcccg gagttgatcg cttcggcgat gcccagccgg gcgctgtgat aggcatcgtc
    40621 gggagtcatc gccggtccga ggcggctcat caccggaaaa taatcgaact ctccgtcggc
    40681 gacgatgccg cgcgcgaagc tgccccacaa atgaaaatga gtatcgacga agcccggtaa
    40741 agcgatgcgg ttgctgccat cgatctgttc ggcgccgggc gcagtaagat tcggacctat
    40801 ggcgagcagc gtgccgttgc gcacgtggac gtcccctcgc gggatgtcgc cgagcttagc
    40861 gtccatggta accacatagg cattgcgaac aacgaattca cttcgggcag gaagcttccc
    40921 cgtcgctcgg tcgattgctg agagtcttga cgcctgcctt tcattcgcgg cgcagctcgc
    40981 tagcccgaga gagcccaatg cggcgattgc ttccaagaaa ccccggcgac ttattctgtt
    41041 ctctttgctc attgacgaac tcctatcttc cgcgccaact taacagaccc gctgtccgcc
    41101 cattgattcg atcgagagta gtcaatgaag gaactcgcag tcaagaaaat ttgagtccga
    41161 tattcccact ggttttagca cagtcgatca taggagcggt gcagcgctgc tcaaggaatt
    41221 gaaattgctc gacagcgggc cgatgattta gatatggttg caggcagata gatttggcga
    41281 tgaagcaatc cgattcgtcc tgctggaggt gacgatgagt tatccaaaga cgttcagaat
    41341 ctcgtgcatg ctggcgtcga tcttcggcac agcgctaatt gccgttgtcc ccgtgttttc
    41401 ccagatgccg tactatgccg gcaagaccat tgcaattatc cgcggcggag gccccggcgg
    41461 ttcgggagag tttcaatcgc gggctctgat gccatacctg cagaaatata tcgcgggaaa
    41521 tccgaccatc gtaatggagt acatgcccgg cgcgggtgga aggaaggcgg cgaattattt
    41581 ctataccgcc aaaccggatg gcttaaagat cgcctccgcc ggcgcgatga ttcccggacc
    41641 tatcttaggc ctgccgggca gcaattatga tatcgataaa tttccttaca taggctcgac
    41701 cgaaaccggc aacccttata ttcttttcac acgcaaagcg gtgggtctcg atactctcga
    41761 taaagttcgt cgcgcttcgg cgctgcgcat aggagctcac tcggtgggac actcggttta
    41821 cgtgactgcg cgcatgttcg cttatatgct ggatctgaaa cagccgaact tcgtcgttgg
    41881 ctttactgat cccgagatgg acgttgccat gcagaacaac gagctggacg ctcgaaccac
    41941 gagcaatacc gatagcctgc tccggaaaga catgatggat aacattcacg ttcacgccac
    42001 catcagcaat cccaagggca gaacggatcc ccgcctgcct ggcctgcctg acctcgacac
    42061 attcgccaag aacgataaag aaagaaacgt catagaatta ttccgagcct tccaagtgcc
    42121 gcgttggccg caccatctct caccgggaac tcctcccgaa ttggtcaaga tccttcgtga
    42181 agcgatggtt aagacgttca aagatcccgc attccagcag gagtttaaaa aactgatggg
    42241 gcgagaaccg acgccgctca ccggagagga tgttcagaag gtggtaaaag agttaccgcg
    42301 cgaccccgaa gtcatcgggt tctataaaaa attagccgag cacgagccgc tgccgccgcg
    42361 ggggtaaatt tgaacgatct aatttaatgt cgaagtgtga tatgcgggcg ctcaggcata
    42421 tttcgaaatc acatacggag gtcccgtgaa gaaattactt ttaacacttt tacttctctt
    42481 tggtcctgta accggactgc acgcccaagc gccgttctat caaggcaaga cgattacatt
    42541 tctcgtcggc tccggctcag gcacggccta tgatatctat tcgcgattgc tggccgccta
    42601 tatcggaaag catctgcccg gcaaccccac tgttgtcgtg cagaatatgc cggccgcagg
    42661 gggcttggtc gcggcgaatt atgtttatgg cgttgccaag cctgacggct tgactctggc
    42721 ttcgatcaat ccggcgcact acttcaatca gctccagggc aataaagaag tgaaattcga
    42781 ctgggcgaaa ttcgcgtggg tcgccagcat ggacaagtcg gagcacatgc tctatatgcg
    42841 cagcgatacg ccctacaaga ccatgcaaga tgtccgcaag gcggccgaac cgcccaaatg
    42901 cggcgccacg ggaacgggaa ccagcggcca ttacatccct cggatgctcg aggagactct
    42961 aggcaccaag ttcactatcg ttacgggtta tgcgggcggc aacgagatcg atctagccac
    43021 tgagcgcaat gaagtcgttt gccgttcatt tactacgcag gcttatttcc agcgcgagcc
    43081 ttatcatacc tggcgcaaga aaaattttgt ccggatactc ctgcaaaccg gccgcacacg
    43141 cgat
//
LOCUS       DASUVS010000272         3531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1423147, whole genome shotgun sequence.
ACCESSION   DASUVS010000272 DASUVS010000000
VERSION     DASUVS010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423147"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VF182_15050"
     CDS             <1..704
                     /locus_tag="VF182_15050"
                     /inference="COORDINATES: protein motif:HMM:NF013527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX6438454.1"
                     /translation="DIRRVLDALNVVALELENYEADDLIGTVCATLCDDNCEVIVVSS
                     DKDLMQLVTEKVSLLDSVKDRWIGAREVIARFGVEPERVPEVLGLMGDAVDNIPGVKG
                     VGQTTAIALMKQFRDLDELYGNLDRLQQSNLRGSARLREALKNGKDTAFLSRDLATIR
                     KNVPIQIDLEELRFKGPSREKMRSLFEELEFNNLVKLLDHGHEFTSDIGLVTLPHKPL
                     PKPTLSVQLPLFSWK"
     gene            769..1920
                     /locus_tag="VF182_15055"
     CDS             769..1920
                     /locus_tag="VF182_15055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6438455.1"
                     /translation="MINPERLKNLLIELIKIDSLSRKEYDVAMRLKREMEDLGAQVSI
                     DDAGEKVGGNVGNLIAHFTGTAPEAMPILLSAHMDTVVPGEGIVPILDGDILRTDGRT
                     VLGGDDKSGVAIICEVLRVIRENGFPCSDVDVVFTICEEAGLIGAKCLDVNRLRARTG
                     LVLDSDSVGFLFTKAPAANRMEFRIHGLEAHAGVCPEKGVNAIKVAADGIAQMSLGRI
                     DHETTANIGVIEGGMAVNIVPNSVILKGEARSHSQEKLEHQTEQMLRCLQDAAARHIL
                     EVGGAQFTATVEAKIERDYDRMDVSDRAPIVQLVRAAAKNLRVDVKTLATGGGCDANV
                     LNQKGLEVANLSTGMREIHTVKEWLDLKDLNLSAQMVLEVVRLNAATNL"
     gene            1976..3421
                     /locus_tag="VF182_15060"
     CDS             1976..3421
                     /locus_tag="VF182_15060"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6438456.1"
                     /translation="MHADEIAVLLKQERGQFLDFLSAYEHRRGGPQKKKEEELGREVA
                     RLLSGMANADGGTLVVGVEADKTLTGLPYDSNELMALLQAPQNLLRPALMPAAETFRV
                     GDLQLLKFEVGSSLEIYRIAGGRSYYRLAAETPALPSEQIHSLKEAKKTVFYERQQAL
                     NATWRDLDSDLVQDFLRKIQDARQPQSVLAQSYHLLDNSRALPAPNMAALLLFAKDPS
                     RWHPRCGIDFVKYEGSERQYGAALNVVKRLRFDGPLVRLIDEAVGRIKEHIRERTILH
                     DLFFRERLEYPTFAWQEAVVNAVAHRDYALSGASIEVWMFDDHITVRSPGLPPLPVSL
                     EQIRQQKSIHFSRNPLIVRVLADMGYLREMGEGVPRMFQEMETYGLNPPEFFTNGFIF
                     TVTLRNAPVYDDDTLRWLNQFAPSSVNQRQRRLLAYAYCHGKTFSTTEYERIAEVDRD
                     TAYRDIRLMVKSGIVAPVKPKSRSYRIIERL"
BASE COUNT          850 a          889 c          998 g          794 t
ORIGIN      
        1 cggatatccg cagggtgctg gacgccttga atgttgttgc tcttgaactt gagaattatg
       61 aggctgacga tctcattgga actgtgtgtg cgacattgtg tgatgacaac tgtgaagtga
      121 ttgtggtttc cagcgacaag gatctaatgc agctggtcac cgaaaaagtt tcactacttg
      181 acagtgtgaa ggatcgctgg atcggcgcac gcgaggtgat agcgagattc ggcgtagagc
      241 cggagagggt tcccgaagtt ttgggattga tgggtgatgc tgttgacaat attccgggcg
      301 tcaaaggtgt tggacaaaca accgcgatcg ctctgatgaa gcaatttcgt gatctggacg
      361 aactctatgg taatctcgat cgcttgcaac aaagcaattt gcgtgggtcg gcccgtctcc
      421 gagaagctct taagaacggc aaggatacgg ctttcctcag ccgagatttg gcgactatca
      481 ggaagaacgt gccgatacag atcgatctgg aagagctgcg atttaagggc ccgagtcggg
      541 agaaaatgcg gtcgctgttt gaggagctcg aattcaacaa tcttgtcaaa cttcttgatc
      601 atggtcatga atttacaagc gatatcggac tcgtcactct gccgcataaa ccgcttccaa
      661 aaccaaccct gtctgtccag ctaccgttat tctcttggaa ataaattgcg tactgtggca
      721 gattggattc catcttcgga gctcaatcac gtgagagcga ggtaaaactt gattaatccc
      781 gaacgtctta agaatctcct gattgaattg attaaaatcg acagcctgtc gcgcaaggaa
      841 tacgacgtcg cgatgcggct caaacgagag atggaagatt tgggcgcaca ggtttcgatt
      901 gacgatgccg gcgagaaagt cggcggcaat gtcggtaatc tcatagcgca cttcactggc
      961 acggcgccag aagcaatgcc catcctattg tcggctcaca tggacacggt cgtgccggga
     1021 gaaggcatcg tcccgattct cgacggggac attctgcgaa ccgacgggcg cacggttctt
     1081 ggcggcgatg acaagagcgg cgtggcgatc atctgtgagg tgctgcgtgt aatcagagaa
     1141 aatgggtttc cgtgcagcga tgttgatgtg gttttcacga tctgtgagga agccgggcta
     1201 atcggcgcca aatgcctgga cgtcaaccgg cttcgcgccc gtaccggact ggtgctggac
     1261 agcgactcgg taggttttct ttttaccaag gcacctgccg ctaatcgcat ggaattccga
     1321 attcacgggc ttgaagcaca cgctggtgtc tgtccggaga agggcgttaa tgcgattaaa
     1381 gttgctgccg acggcatcgc acagatgtcc ttggggcgca ttgatcatga aactaccgcc
     1441 aatatcggcg tcatagaggg aggcatggcg gtcaacatcg tgcctaactc agttatctta
     1501 aagggtgagg cgcggagtca tagccaggag aaattggaac accaaacaga acagatgcta
     1561 cgctgcctcc aagatgccgc ggcccgtcac atattggaag tgggtggagc gcaattcacc
     1621 gccactgtgg aggcaaaaat cgagcgggat tatgaccgca tggatgtttc agatcgagct
     1681 cccatcgtgc aactcgtacg cgcggcggcc aagaatttga gggtcgacgt caaaactctc
     1741 gccaccggcg gtggatgtga cgccaacgta ctcaaccaga agggattaga ggtcgctaat
     1801 ctatcaaccg gcatgcgcga gatccacact gtaaaagaat ggctcgacct caaggatctt
     1861 aatctgtccg ctcagatggt cctggaagtg gttcgtctta acgccgcgac gaacctgtaa
     1921 aaaggatggc gaagggcggc actctgggac gagattccgg ctctaaactt gatttatgca
     1981 cgccgacgaa attgcggtcc tgcttaaaca ggaacgcgga cagtttctgg atttcctgag
     2041 cgcctatgag catcggcgtg gcgggcccca aaagaagaag gaagaggagc tcggtcgaga
     2101 agttgcgcgc ttgctctccg gcatggcaaa cgccgacggc ggaacactgg tggtgggtgt
     2161 cgaagccgac aagacgttaa ccggactccc ctatgattct aatgagctca tggcattgct
     2221 gcaggcgccg caaaatctac ttcgaccggc tcttatgccg gcagccgaaa catttcgggt
     2281 aggcgatcta cagctcctca aattcgaggt cggttccagt cttgagatct accgtattgc
     2341 cggcggccga tcctactatc gactagcggc ggaaaccccg gcactgccct cggaacagat
     2401 tcattcgctc aaagaagcca agaagacggt cttttatgag cgacagcagg cgctcaatgc
     2461 cacgtggcgg gatctagatt cagaccttgt ccaagatttt ctgcgtaaaa ttcaggacgc
     2521 gcggcagccc caaagtgtcc ttgcccagtc ttatcatttg cttgacaatt ctcgcgcgct
     2581 tcctgctccg aacatggcgg cattgcttct gttcgctaaa gacccgagtc gctggcatcc
     2641 gaggtgcggc attgacttcg tcaagtatga aggttccgag cgtcagtatg gcgcggcgtt
     2701 gaatgtcgtt aaacgactac ggtttgatgg gccgctggtt cgcctgatcg atgaagcagt
     2761 tggccgcatc aaggagcata tccgcgagcg gaccatattg catgatcttt tctttcgcga
     2821 acgtctggaa tatcccacct tcgcctggca ggaagccgtt gtgaacgcgg tcgctcaccg
     2881 cgactatgcc ttgagcggcg cttccatcga agtttggatg tttgacgatc acataacggt
     2941 gcgaagcccc ggccttccgc cccttcctgt gagtctcgaa caaatacgcc agcaaaagag
     3001 cattcacttc tcccgcaatc cactcatcgt gcgcgtcctg gcggatatgg gctatctgcg
     3061 cgagatgggt gaaggggtgc cgagaatgtt tcaggagatg gaaacctacg gacttaatcc
     3121 gccggaattt ttcaccaatg gatttatctt caccgtaacg cttagaaatg cgccggtcta
     3181 cgacgacgac actttacgct ggctcaacca atttgcaccg tcgtcagtca accagagaca
     3241 gcgccgcctg ctcgcctacg cctattgtca tggcaagact ttctcaacaa cggaatacga
     3301 gagaatagcg gaagtcgatc gggacaccgc ctatcgagat atccgcctga tggttaaaag
     3361 cggcatcgtg gcgccggtga aaccgaaaag ccgaagctac cgaatcatcg agcgcttgta
     3421 agagatcgat gtggattatt ccgatctgat gaaattggcc agcggccacg tcgaagcccg
     3481 aatagttcag gcggcgttag agctggagat tttcgaaaat atagcaggcc a
//
LOCUS       DASUVS010000273         5637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1423169, whole genome shotgun sequence.
ACCESSION   DASUVS010000273 DASUVS010000000
VERSION     DASUVS010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423169"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VF182_15065"
     CDS             complement(<1..921)
                     /locus_tag="VF182_15065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6438457.1"
                     /translation="MSDDASKTSGGQPLLSREPSKAGAPLRILVVEDDELDRRAVRRC
                     LQQCGIAVTVEYASSAQETLQAIRSVPYDCVLLDYYIPGVAGLALFQKIREVAPNMPV
                     VIFTGRGDEDIAVELMKAGAADYLPKASLTAERLASSLRHIIELSEAAEARRRAEEEL
                     RAQEARFRTLVNAIPQLAWMADGTLARYWFNDRWYDYTGTSFEEVRGWGWQKVHHPDH
                     LQRVLESMQRSFDAGAPWEQTYPLRGRDGTYRWFLSRALPIRGEDGSIASWLGTNTDI
                     TEQKNAEAERERALALEQQLRSQAERATRAR"
     gene            complement(918..1334)
                     /locus_tag="VF182_15070"
     CDS             complement(918..1334)
                     /locus_tag="VF182_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017314585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6438458.1"
                     /translation="MNSSSLNILLVEDDDVDVMNVKRAFDKNHITNPLYVAGNGLEAL
                     EKLRSGEIPQGRRLVLLDLNMPKMNGIEFLRELRKDPALAPTPVVVLTTSNNDRDKID
                     AFNFNVAGYLVKPVTFAEFSELMVTLNKYWTLVELP"
     gene            complement(1331..3043)
                     /locus_tag="VF182_15075"
     CDS             complement(1331..3043)
                     /locus_tag="VF182_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016556058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6438459.1"
                     /translation="MSTTKPQDSSDLYRRLVESVSDYAIFALDAKGYITSWNPGAQRI
                     KGYTGEEIIGRHFSSFYPPEDIASGKPAWELEVAAREGRIEDEGWRLRKDGSRFWANV
                     VISAVRNNHGEVTGFSKVTRDLSDRRNTEEALRRSEERFRLLVQSVKEYAILMLDPEG
                     HVASWNEGAQHIKGYTADEILGRSFTLFYPPEAVADGFPQHELEVAALEGRFEDEGWR
                     VRKDGSRFWANVVITALRNSEGRLVGFAKVTRDLTARREAETQARRLAAEQAAHAEAE
                     RRSAELAELNERLQEQAIELETQAEELRILAETLHHRNEELGAALADAKTAREAAERS
                     AAAAIDAYRELDQFAYVASHDLKAPLRGIANLAQWIQDDTGEQLGAESAEHMRLLQGR
                     VHRMEALIDGILAYARAGRMLNSPERIDTGALVREVIELLAPPEEAIIEVPSQMPNVT
                     AERVPLQQVFLNLIGNALKHTRVERSDVVVRIGWRDLGDAFEFSVSDNGPGIDPEFHE
                     RIWGIFQTLAPRDKVEGTGIGLSVVRKIVETRGGSTSVESSANQGATFRFIWPKTIRQ
                     EALA"
     gene            3386..3823
                     /locus_tag="VF182_15080"
     CDS             3386..3823
                     /locus_tag="VF182_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438460.1"
                     /translation="MKTVKRGQENSQDSAIRQPLLEFRHALLDLHKQLIDSERAVYEA
                     DVGPVQSPHHFFQLLTSDPWFAWLRPVSQLIVMIDEVLDGPEPLTNNNIDAVIMESVF
                     LLVPAKAEGEFSTRYLAALQRDPAVVLAHAQVAKRIPPGKPRA"
     gene            complement(3915..4910)
                     /locus_tag="VF182_15085"
     CDS             complement(3915..4910)
                     /locus_tag="VF182_15085"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6438461.1"
                     /translation="MINTIRFALSLLSLTLAVIWSTPVRGQLTRINVGYSAISGDALP
                     AWIAKDAGIFEKNGLDVQLVFFSGGTTAVMALIAADTPIAQLAGPAVVHSAAAGSDAV
                     LIVGGVTSLNYYLMGRPEIKTPEQLKGGSVAISRFGSSSDFIARYALSKVGLTPGKDV
                     TIVQIGSTTARVDAALSGRVQATVVQPPASIIAQKKGMNILADLPKLGLVYQHTSAAT
                     TRRYIREHSDVVRRYVKSQVDAVHRIYTDKEASIKALAKYIGRNVDRDVLEKTWENLL
                     SESVLPKKQYPSLEGIKTILNAELKGKAANPENFADSTFIRELDKSGYIDALYKK"
     gene            5201..>5637
                     /locus_tag="VF182_15090"
     CDS             5201..>5637
                     /locus_tag="VF182_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004441674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6438462.1"
                     /translation="MIKINQLSKSYFARELFTDVSLQINAGDRLGLVGRNGHGKTTLF
                     KLILGQEEPDSGTITIPRNYRIGHLEQHLNFTEPTILAEAALGLPEDESHSLYKAEAI
                     LFGLGFSAGDLEKAPGEFSGGFQIRINLAKLLVSEPNLLLLDEP"
BASE COUNT         1161 a         1650 c         1592 g         1234 t
ORIGIN      
        1 tcgcgctcta gtggcgcgtt cggcctggct gcgcagctgc tgctccaaag caagcgctcg
       61 ctcgcgctcg gcctcggcgt ttttctgctc cgtgatgtcg gtgtttgtgc cgagccagct
      121 ggcgatgctg ccatcttcgc cccggattgg cagagcccgg gacagaaacc agcggtaggt
      181 tccatccctt ccgcgcaggg ggtaggtctg ctcccagggc gcgccagcat cgaagctgcg
      241 ctgcatgctc tcaagaaccc gttgcaaatg atcggggtga tgcaccttct gccaacccca
      301 gccccgaacc tcttcgaagc tggtgccggt gtaatcgtac catcgatcat tgaaccaata
      361 acgggcgagg gtcccgtcag ccatccaggc gagctggggg atggcgttga ccaacgtgcg
      421 aaagcgcgcc tcctgcgcac gcaactcctc ttcggcgcgc cgccgagcct cagcggcctc
      481 ggaaagttcg atgatgtgac gcaggctcga tgccaatcgc tcggctgtga gcgaggcctt
      541 gggcagataa tccgccgcgc cggccttcat caattcgacc gcgatatctt catcgccgcg
      601 tccggtgaag attaccactg gcatgtttgg cgccacttct ctaattttct ggaagagcgc
      661 gagccctgct acgccgggga tgtaatagtc gagaagcacg caatcgtaag gcactgaacg
      721 aattgcttgc agcgtctcct gtgccgaact cgcatactcg acggtcaccg caataccgca
      781 ctgttgcaaa cagcgccgca cggcgcggcg gtctagctcg tcatcctcga caacgaggat
      841 acgcagcggc gcgccggctt tcgagggctc tcggcttaag agcggctgcc cgccggaagt
      901 tttagaagcg tcgtcgctca tggcagttca acgagcgtcc aatacttgtt gagtgtcacc
      961 atcagctcgg aaaattcggc gaacgtaacc ggcttcacca agtaaccggc aacgttgaaa
     1021 ttgaaggcgt cgatcttgtc gcggtcattg ttggacgtgg tgaggacgac cacgggcgtc
     1081 ggcgcgagcg ccggatcctt tcgcagctcg cggaggaact cgatgccgtt cattttgggc
     1141 atgttgagat cgagtagaac gaggcggcgg ccttgcggaa tctcgccgct gcgcagcttc
     1201 tcgagcgcct cgagaccatt gcctgccacg taaagcgggt tggtgatgtg gttcttgtcg
     1261 aacgcgcgct tcacgttcat cacatcgaca tcgtcgtcct caaccagcag gatgttgaga
     1321 gatgaactgt tcatgcgagc gcctcttgtc gaatagtctt gggccagata aagcgaaacg
     1381 tcgcgccctg attcgcagaa gattccacag atgtagagcc gccccgggtc tcgacgattt
     1441 ttcggaccac cgaaagcccg atgccggtgc cttcaacctt gtcgcgtggc gccagagttt
     1501 ggaaaatgcc ccagatccgc tcgtggaact ccggatcgat gccggggccg ttgtcgctca
     1561 ccgagaactc gaacgcatcc ccgagatccc gccaaccgat gcgaacgact acgtcagagc
     1621 gctcgaccct cgtgtgtttc agcgcgttgc cgataaggtt taggaacacc tgctgcagcg
     1681 gcacgcgttc ggcggtcaca ttgggcatct gcgacggcac ctcgatgata gcctcttccg
     1741 gcggagccaa gagctcgatg acctcgcgca ccagcgcgcc ggtgtcaatc cgttctggcg
     1801 aattcaacat ccggcccgcg cgcgcgtagg ccaggatgcc gtcgatcagc gcctccatgc
     1861 ggtgcacgcg cccttgcagc aggcgcatgt gctcggcgga ttcggcgcca agctgctcgc
     1921 cggtatcgtc ctgaatccac tgtgccaggt tagcaatgcc gcgcaatgga gcttttaaat
     1981 catgggatgc gacgtacgcg aactgatcga gctctcggta cgcatcgatg gcggccgcgg
     2041 cggagcgctc cgccgcctcc cgcgccgttt tcgcatcggc aagcgccgcc ccgagttcct
     2101 cgttcctgtg atggagcgtc tcggccaaaa tgcgcaactc ctcggcctga gtttcaagct
     2161 cgattgcttg ttcctgaagc cgctcattga gttcggccag ctcagcgctg cggcgctcgg
     2221 cttcagcatg ggccgcctgt tcggcagcga gccggcgcgc ctgcgtttcg gcctcgcgac
     2281 gggcggtgag atcacgggtg actttagcaa atccaaccag ccgcccttcg ctgttgcgca
     2341 atgcggtaat caccacattg gcccagaagc gcgacccatc tttgcgcacg cgccagcctt
     2401 cgtcctcgaa acgcccttcg agcgcagcca cttccaattc gtgctgtgga aaaccgtccg
     2461 cgaccgcctc gggcgggtag aacaaggtga aggaacggcc gagaatttca tcggcggtat
     2521 atcccttgat gtgctgcgcg ccttcgttcc agctcgcgac atggccttcg ggatcaagca
     2581 tcagaatagc gtactccttg acgctctgga ccagcaggcg gaaacgctcc tcgctacgcc
     2641 gcagcgcttc ttcggtgttg cgccgatcac ttagatctcg ggttactttg gaaaaaccgg
     2701 ttacctctcc gtgattgttc cgaaccgcgc tgatcacgac attggcccag aaccgtgatc
     2761 catccttgcg cagccgccat ccctcgtctt cgatgcgtcc ctcgcgagcc gcgacttcga
     2821 gctcccaagc cggttttccc gaagcgatgt cctcaggtgg atagaaggaa gaaaaatgcc
     2881 gcccgatgat ctcttctccc gtgtatccct tgatgcgctg cgcccctggg ttccagcttg
     2941 taatgtaacc tttggcatcc agcgcaaaga tggcatagtc cgatacactc tctaccagcc
     3001 ggcggtataa atcagaggag tcttgcggtt tcgtcgtaga cattgcgctg gggtcgattg
     3061 tcctccttca agttgcgagc gcctgcccgc tcagttgagg cggtctacgg gcgataactg
     3121 aagatcgttc accagtcaag tcggatcaga cttaatcgtt tacgctgtaa cattccgggc
     3181 ccggcagttc aataatcgga cccgtttgcg gcgcaacctc aatattgaaa atgccgtcaa
     3241 attcgctctc ttaggtgtca ggaggagatc cgtttccgac agtgatggga gagctgttcc
     3301 agtccaactg cgagcacctt ttatctcatc gatgggcaac tattcgactc acacaaaacg
     3361 ggatttggcg tgttatgatc aggctatgaa aacagtcaaa cgagggcaag aaaactcgca
     3421 agactcagcg attcgccagc ctttgctgga gttccgccat gccttgctcg atttgcataa
     3481 acaattgatc gattccgaac gcgcggttta cgaagccgat gtcggaccgg tgcaatcgcc
     3541 gcatcatttc tttcagcttc taaccagtga cccgtggttt gcctggctgc gcccggtatc
     3601 gcaactgatc gttatgatcg acgaagttct cgatggcccg gaaccgttaa ccaacaataa
     3661 tatcgacgcc gtgatcatgg aatcggtatt tctgctggta ccggcgaaag ccgaaggcga
     3721 attcagcacg cgttatctgg ccgccttgca gcgcgatccc gcagtcgtgc tcgcccatgc
     3781 gcaagtggcc aagcgaatcc ctcccggcaa gccgcgagcg tagaaagccg gttcggcgca
     3841 tgctctgcgg cttagcagac gggtcggatc cgagcgccga gatcagaagc taaacgcaac
     3901 accaaacgtc agtactattt tttgtagagg gcatcgatat agccgctttt gtcgagctcc
     3961 ctgatgaaag tcgaatcagc gaagttctcc ggattagccg ccttgccttt tagttctgca
     4021 ttcaagatgg tcttgatgcc ctcgagggaa ggatactgtt tttttggcag aaccgactcg
     4081 ctgagcagat tttcccaggt tttttcgagg acatcgcgat cgacgttacg gccgatatat
     4141 ttcgccagcg ctttaatgga agcttcttta tcggtgtaga tccgatggac ggcatccacc
     4201 tgggatttga cataacgcct gacgacgtct gaatgctcgc gaatatatct cctggtggtc
     4261 gcggcgctgg tatgctggta aaccagaccc agttttggca gatcggctag aatattcatg
     4321 cccttcttct gggcaatgat gctggcgggc ggctgaacca ccgtcgcctg cactctgcct
     4381 gaaagggcgg catcgacccg tgcggtggtg ctgccgatct ggacgatcgt tacatcctta
     4441 cctggcgtca ggcccacttt agataaagcg taccgggcga tgaaatccga agaggaaccg
     4501 aagcgactga tcgcgacgga gccgcctttg agctgctccg gtgtttttat ttcaggccgt
     4561 cccatcaaat agtaattcag cgaggtgacg ccgccgacaa tgagaacggc gtctgacccg
     4621 gccgcagcgg aatggacgac ggcaggtccc gccaattggg cgatgggcgt atcagccgca
     4681 atcaacgcca tgaccgcggt cgtgccgccg gaaaaaaaca ccagctgaac gtccagcccg
     4741 ttcttttcaa atatcccggc atctttggcg atccaggccg gcagcgcatc gccgctgatg
     4801 gcgctgtagc caacattgat gcgggtcagc tggccacgaa ccggagtgct ccagataacc
     4861 gctaacgtca aagagagaag tgagagcgca aagcgtatgg tgttgatcat agcggctcct
     4921 tactgttgag ctgcggcacg ccgcagggtc agtagatatc ttgcgccgga aaccatgaaa
     4981 ggacgctata ccttgaccat ctgcaaggtc aaggaacaag gcagtatggg ttttcgcgcg
     5041 cggtgtaaag gcttgcaact tcgttttaat tggaagctga tgcagagctg gaagcgcagc
     5101 agcgaattcg gttgtcgtgc tggaaaacgc gcgcgatcac agctaaaata tgcgattcta
     5161 acatttgata cgcgcacgtt tactctactc gggttttgca atgatcaaga tcaaccaact
     5221 atccaagtcg tactttgctc gcgagctctt taccgacgtc agtttacaaa taaatgccgg
     5281 cgatcgtttg ggcttagtgg gccgaaacgg tcacggcaaa acgaccttat ttaagcttat
     5341 cttgggacag gaagagccgg actcgggcac gatcacgatt ccgcgtaatt atcgcattgg
     5401 ccatctcgag caacatctga actttaccga gccgacgata ttggcggaag cggcgttagg
     5461 actgccggaa gatgaatctc attccctcta taaagcggaa gccatcctgt tcggcctggg
     5521 gttttccgcc ggcgatctgg aaaaagcgcc gggagaattt tccggcggtt tccaaattcg
     5581 tatcaatctg gccaaactcc tggtttccga gcccaacctg cttttgctcg acgagcc
//
LOCUS       DASUVS010000274         5372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1424041, whole genome shotgun sequence.
ACCESSION   DASUVS010000274 DASUVS010000000
VERSION     DASUVS010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424041"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VF182_15095"
     CDS             <1..200
                     /locus_tag="VF182_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEX6438463.1"
                     /translation="SRSYMDVQDLAKRIRDTCIEAVLQAYEDAGVQGLCAEGQWEAAV
                     DALRTVDLAPLLSELKEPLAD"
     gene            506..1186
                     /locus_tag="VF182_15100"
     CDS             506..1186
                     /locus_tag="VF182_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438464.1"
                     /translation="MKQHIFLLAAVAALGVASSDSSQSIGAERAQANSAMSFFVTSTG
                     SKTANLGGLSGADKICQGLADAVGLGNKTWHAYLSVEHDSNNGNKATDARTRIGTGPW
                     SNAKGVVVAKDLDELHARKGDADVFLDEHGQRVPGNWPDSPKPVVHDILTGSTADGKV
                     TVGKTCDDWTSESKDQQAQVGHSDGLGPGGDPSGRYASWNSSHENGSCADTAPRGGGG
                     RLYCFATK"
     gene            1458..2981
                     /locus_tag="VF182_15105"
     CDS             1458..2981
                     /locus_tag="VF182_15105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6438465.1"
                     /translation="MFLRKSLVVTCAALLSLASAASAAEKLSPGKEKEFLFKVVERNS
                     KAIALLGDNIYYFAELGLQEFETAKLMTQILEDAGFKVQRGISGMPTAFMATYGSGQP
                     VIAIHTEYDTTPGNSQTPGVPEHKPLVEGAPGHAEGHNVNAAVMVGAAFAVKKAMDEY
                     KLPGTLKVFGAPAEELLIPRPYFVRDGYFKDVDVAFHTHIGEELRTEYGLRQYALISA
                     EFTFKGQSAHSASAPWAGRDALDGVELMSMGFDKLREHLEPTHRSHRVINYGGDQPNV
                     IPGLAKIWWFFRESTIDKAQANFEKAKKVAQGAATMTDTEYSVQVFSAIWPTRGNRTL
                     AEVTQKNVELVGMPKWTEEEQNLAKALQRNLKGKEEGLHKEISPLRESRQGASSNDAG
                     EVTWIVPTGRVTFPSNIPNIPNHHWAAGVAPATSIAHKGAVAGAKVLAASIVDFLIDP
                     KLVEEAKTTFKKETAGFQYKSFLPPEQKPPTYLNKELMERYRPLLSKYYLKDKPNFE"
     gene            3377..4579
                     /locus_tag="VF182_15110"
     CDS             3377..4579
                     /locus_tag="VF182_15110"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX6438466.1"
                     /translation="MNQADAGLPQISVNERDRRYGLIRQEMRNANIDCAIVTGTNLFY
                     LTNGIPGEMFGLLPATDDLPTLIINFRQLADISVDVLLNMQEWIQDIRGGVDGSQVIA
                     RIKELKLEKATLGLAGYATINQAFYAQLQKTFPEAKFADVSSIFTNARTIKSEEEIAM
                     IDMANRIFDRAVERVCEVTRPGMLGRRVLQEGINSMWAAGGDMHSSFNLHFGPVPKQN
                     PVLMQISLDRVIQKGDIGTLTAHAEYRHYAGHSDHQLSVGEPKPLHRELFQAVLNVRE
                     KVLSTVREGLSHLDVVESYRKAVAETGFRSSPHSQTHQYGIDVPEFPGPAFNIPDPSK
                     EQLSKTLGLGFRGSNFTLKAGMVYSISPTLLAHSGDDTMLAGTTVVVTKDGYRELGDR
                     KLELSVCA"
     gene            4812..>5372
                     /locus_tag="VF182_15115"
     CDS             4812..>5372
                     /locus_tag="VF182_15115"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX6438467.1"
                     /translation="MSEKIYNTQVLIVGAGPVGLALALELGLRGINCLIVEQRDGKVT
                     LPKMNTVSTRTMEFCRRWGVAAKIKQAGIGEDYPLDIRFITSMAGYELLRFRYPTYRE
                     RGKLDYTPEGNCVCSQLWFDPILLERTRSFPNVEVHLRTRLESLSQSEQLVTAELLDV
                     ETNQRKTVRAPYLIGCDGADSSVRELS"
BASE COUNT         1312 a         1418 c         1524 g         1118 t
ORIGIN      
        1 tctctaggag ctatatggac gttcaagatc tcgccaaacg tattcgagac acatgcattg
       61 aagcggtcct gcaggcatac gaggatgccg gtgtacaggg cctctgtgcg gagggacagt
      121 gggaagcggc tgtcgacgcc ctgaggaccg ttgatctcgc gccgctacta agtgaactga
      181 aagagccgct cgcagactga tcgtccctcg tagcgcatcg cgtgattagt tccggctggg
      241 ataagtgatc ccttgcaagc ggatcgccag gaatgttgaa cgtcgcggcg gaccttgatt
      301 gcggcgcgaa gacatcgaat ccgcggcaat caaaaaatcg gcagcgaaga aaaatgctag
      361 tcgagacacc gcaaagaacc ggtgacgatt gccgatattc aattgattta tgttttctcg
      421 ttacgatata accgccatga atgctttgat tcgacggtta cggccctcga acaatgccaa
      481 aatttccaac gaaaaaggac ctcctatgaa gcaacatatt tttcttcttg ccgccgtcgc
      541 ggcgctcggc gtggccagca gcgattcgag tcagagcatc ggcgccgagc gcgcgcaggc
      601 caattcggca atgagttttt ttgttaccag cactgggagc aagacagcca acctgggcgg
      661 gctgagcggc gcggataaga tttgtcaggg actggcagac gcagtcggtc tcggcaataa
      721 gacttggcat gcctatctga gcgtcgagca cgattccaat aacggcaaca aggcgaccga
      781 cgcacgcact cgcatcggca ccgggccttg gtccaatgcc aaaggtgttg tagtcgcgaa
      841 ggatcttgac gagctgcacg cgcgcaaagg cgacgccgac gtctttctcg acgagcacgg
      901 gcagcgcgtc cccggcaatt ggccggattc accaaaacca gtggtgcatg acattctgac
      961 cggctcgacg gcagacggta aggtcacggt tggaaaaacg tgcgacgact ggacgtcgga
     1021 atctaaagat caacaggctc aggtgggtca ctcggacggc ctcggaccag gcggtgaccc
     1081 gtcggggaga tacgccagtt ggaactcatc ccatgagaac ggaagttgtg ccgataccgc
     1141 tccgcgcgga ggcggcggcc ggctttactg ctttgcgacg aaatgacgcg atcatcgctt
     1201 gagacggtca gcgcggccaa atctatagtc tttgccgcga tcgttggggt aatttatttc
     1261 atagttggtg aagtggccca ggaagcgcta ccagatccat tgctttacag actcttctct
     1321 taatagccgc gtgatttgct cttgatcttc tcagtgcctt cggcgccggc agccttttct
     1381 tgactggttc caaccggctg gtatacggtt ttgcttgctc gataatgtgc catgccgcaa
     1441 actgaggagg ccaatccatg ttcttgagga aatcactcgt agtcacatgt gccgcgctgc
     1501 tgtctttagc atccgcggct agcgccgcgg aaaaactaag tcccggcaag gaaaaggaat
     1561 tcctgttcaa agtcgtggaa cgcaacagca aggcaatcgc cctactgggc gacaacatct
     1621 attattttgc cgagctcggg ttgcaggagt ttgagactgc gaaactgatg acgcagatcc
     1681 tcgaagacgc cggcttcaag gtgcaacgcg ggatctcggg aatgcctact gcattcatgg
     1741 cgacctacgg ttcgggtcag ccggtgatcg cgatccatac cgaatacgac accacaccgg
     1801 gcaattcgca gacaccgggt gtgccggagc acaaaccgct tgtcgagggc gctcccggcc
     1861 acgccgaagg gcacaatgtc aacgcggcgg ttatggtcgg cgcggcgttc gcggtgaaaa
     1921 aagcgatgga cgaatacaaa ctgccgggta cgctaaaagt ttttggcgcg ccggccgaag
     1981 aacttttaat tcctcgcccc tacttcgtcc gcgatggcta cttcaaggat gtcgacgtgg
     2041 catttcacac tcacatcggc gaagagctga gaacggaata tggtctgagg cagtacgctc
     2101 tgatctcggc ggaattcacg ttcaaaggcc agagtgccca ctcggcgtcg gcgccctggg
     2161 cgggccgcga tgcgctggat ggggtcgagc tgatgagcat gggtttcgat aagctgcgcg
     2221 agcacttgga gcccacacat cgaagtcatc gcgtgatcaa ttacggcggc gatcaaccca
     2281 acgtcattcc aggactggcg aaaatctggt ggtttttccg cgaatcaacc atcgataagg
     2341 ctcaagccaa tttcgagaaa gccaagaaag tcgctcaagg cgcggctacc atgacggata
     2401 cggaatatag tgttcaggta ttcagcgcaa tctggccgac gcgcggcaac cggacactgg
     2461 cggaagtgac gcagaaaaac gtcgagctgg tcggcatgcc taaatggacc gaggaagaac
     2521 aaaatttggc gaaggcgttg cagcgcaacc ttaaaggcaa ggaagaggga ctgcacaaag
     2581 agatttcgcc gctgcgcgaa tcgcgccagg gggcgtcgtc gaacgatgcc ggagaggtga
     2641 catggatcgt gccgacgggc cgagtcactt ttcccagcaa catcccaaat attccgaatc
     2701 accactgggc cgccggcgtc gcgcctgcga cttcgatcgc gcacaaagga gcggtagccg
     2761 gcgctaaagt cctggccgct tccatcgtcg atttcctgat cgatcccaag ctggtggagg
     2821 aagcaaagac tactttcaaa aaggagacgg ctggatttca gtacaagtct tttctaccgc
     2881 cggagcaaaa gccgccgacg tatttgaaca aggagcttat ggaacgttac cgtccgctgc
     2941 tctccaagta ctatctcaaa gacaagccga actttgaatg agcgtgtcgg aatcagaatg
     3001 cgctccatga tctgaagcaa tgcggcgcga ctcgacccgt accttccctg cccattgccc
     3061 gaccgcgact gaaatggagc ctaacgatcg agacgcgaat gtgcgtagcg ccacgaatct
     3121 gccgagaccc tgagcaagcc atcaaagggt gtgtccagct ctaacaccgc aaacatggcg
     3181 caccaaccga cagcgcgcga acgcacagaa ccgtaagaac tctcctgtcc cacggaaaaa
     3241 cccatccggt aagctcgcag aggtaatggt aagccagaac aggacgttga cgtttgattg
     3301 cttgattgca tttagcttgc gaataaaccg acatccatag tacgagagca atgaaatata
     3361 gagtaatgga ggctccatga atcaggccga tgccggactg ccgcagattt ccgtgaatga
     3421 gcgtgatcga cgctatggtt tgatccgaca ggaaatgaga aatgcaaata tcgattgcgc
     3481 gattgtgacg ggtaccaatc tgttttatct cacaaacggt attcccggtg aaatgttcgg
     3541 tttgctgcct gcgacagatg atcttccgac gttgattatc aattttcgcc aactggccga
     3601 tatttccgtc gatgtgctgc taaacatgca agagtggatt caggatatca ggggcggtgt
     3661 ggatggttca caggttatcg cacggatcaa ggagctcaag ctggagaaag caaccttggg
     3721 gttggccggc tacgccacta tcaatcaggc tttttatgct caacttcaaa aaaccttccc
     3781 cgaggccaag tttgccgacg tatccagcat ttttaccaat gcaagaacta tcaaaagcga
     3841 agaggaaatt gccatgatcg acatggcaaa caggattttc gatcgggcgg tggaaagagt
     3901 ctgcgaagtc accaggccgg gcatgctggg ccgacgggtg ctgcaggaag gtatcaactc
     3961 gatgtgggca gcgggcggcg atatgcattc aagttttaac ctacattttg gtcccgtgcc
     4021 gaagcaaaac ccggtgctca tgcaaatttc cctcgatcga gtgatccaaa agggagacat
     4081 cggaactctc acggcccacg ccgaatatcg tcactatgcc ggccacagcg atcaccagtt
     4141 gtcggtcggt gaaccgaagc ccctacatcg ggaattgttc caagcagtat tgaacgtgag
     4201 agagaaagtt cttagcaccg tcagagaggg cctatcccat ttggacgtgg tggaatcata
     4261 ccgcaaagct gtggcggaga ccggatttcg atcctcgcca cattcgcaga ctcatcaata
     4321 tggcatcgat gttcccgaat ttcctggacc ggcgttcaac attccggatc cgtcaaagga
     4381 acaactcagt aagaccctcg gcttgggttt tagaggaagt aatttcacgt tgaaagcagg
     4441 catggtctac agcatctcgc cgactttgct cgcccattcg ggggacgaca ccatgctggc
     4501 aggcaccacg gtagttgtca ctaaagacgg ctaccgtgag ctaggcgatc gcaaactgga
     4561 attgtctgtt tgtgcatgac gaaatttctc gcgcacagtg atggggtgct gcaagcgagc
     4621 ggagatcaaa agccgacaca acagcaatgc taatcgccaa accgtgaact acgagggatc
     4681 cttcagtgac aatctggtat cggcgatcaa gcgctttcgc gagagagcca ccgctaactc
     4741 gtactttgtg gcggtccgag acagggatag ctttttgctt tgccaatcgg cgctttgtcg
     4801 gtagttgagt gatgtcagag aagatctaca acacacaagt tttgatagtc ggggctggtc
     4861 cggttggact agcgttggct ctcgagctgg gtttgcgcgg aatcaactgc ctaatcgtcg
     4921 aacagcgtga cggaaaagtc acattgccca agatgaatac ggtcagtacg cgaacgatgg
     4981 aattttgccg gcgctggggc gtcgctgcga aaatcaagca ggccggcatc ggcgaagact
     5041 atccactcga cattcgattc atcaccagca tggccggcta cgaactgctg cgcttccgct
     5101 acccgaccta tcgcgagcgc ggcaagctcg attatacgcc ggagggcaac tgtgtctgct
     5161 cgcagctctg gttcgatccg atactgctcg agcgaacgcg ctcgtttcca aacgtcgagg
     5221 tgcatctgcg gactcgcttg gaatcattat cgcagtcgga gcaactcgta actgcggagc
     5281 tgctcgatgt cgaaaccaat cagcggaaaa ccgtgcgcgc gccgtatttg atcggctgcg
     5341 atggcgccga cagttcggtg agagaacttt ca
//
LOCUS       DASUVS010000275         9681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1424480, whole genome shotgun sequence.
ACCESSION   DASUVS010000275 DASUVS010000000
VERSION     DASUVS010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424480"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VF182_15120"
     CDS             <1..1167
                     /locus_tag="VF182_15120"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6438468.1"
                     /translation="MRLLGILTGGHFVIHWFQQFFPVILPSIKSGLSLTNIEVGALTS
                     AQQMIMGLGQLPVGLLADSMVRHRAKMLGLSLVAMGAAYFLLGSPGFVWALLGSGLIG
                     LGTALWHPTAAASLSNRFPEHRATALSIHGTGATISDTLTPLFVGALLASFSWQNVAR
                     LQLLPGLLFAVLVWRALAEAFSDGDPRPRQGSTQIREVMGVVRNPAFLGVSAATGLLS
                     MGRLVILTFLPIYLQEHLHYSAFALGVFVALMHALGTISQPALGLLSDRFGRKTVLMP
                     SCLLLGLFFALLAVVPAGIPLALVIIAIGLFFYTLFNIMNAAVMDVAGAAVQASTYGL
                     MTLITQLVVIPTPMVTGYLIGEFGIKFSFVLAGVFLIIAGLVLAPLKLYRGSRA"
     gene            1324..2538
                     /locus_tag="VF182_15125"
     CDS             1324..2538
                     /locus_tag="VF182_15125"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6438469.1"
                     /translation="MKLSRAKVLLLSTAVVTMTSIGMSSFSLFLRPMEADFGWSRSMV
                     TLPYMFAMLGWGAGAVLFGKLADDYGARRVILGGILLMASGFFGMSLSRNVWQLSLTY
                     GLMVGIAMGACGLAIASLLVSQHYESKSRGLAVSVLQTSAPLSPLLFAAPLYFLINRF
                     GWRAGALSTAVLLIAVALPLAWLGAREPHGSGVRRARLGWRACLPYLRNRSVLVMFAA
                     RFSCGVAFFQIAHLVALALSKGFTNATGAAAVSVFGAAAAASALLFGWLSDRYGRAQM
                     LALSYIVRGVGTFFLSFDVPNELVFFILVAVAIGPTFGTVAVQNVMFYEIAGPRLAGV
                     ILGLSFIVHQIGSAGGPMFASVVFDRTGSYDGFMAVMALILIAAGGLVYSNINQQTQF
                     PELVFGSSPARS"
     gene            complement(2578..3000)
                     /locus_tag="VF182_15130"
     CDS             complement(2578..3000)
                     /locus_tag="VF182_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEX6438470.1"
                     /translation="MSGNPVSSEKEKLLQSRMRALGVREQDLEEQFVRSSGAGGQNVN
                     KVSSCVLLHHRPTGIRVKCQQERSQALNRFLARRILLDKIESKIKGAQSAEEQEIAKI
                     RRQKRKRSRRAKLKLLADKRHLAEKKSRRAPVRPDQQD"
     gene            complement(3030..3377)
                     /locus_tag="VF182_15135"
     CDS             complement(3030..3377)
                     /locus_tag="VF182_15135"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX6438471.1"
                     /translation="MSAMRCPKCRTETMNVFSVEGVALERCSSCSGIWFDARELSQLL
                     EQDARHLSSLRRGRDTKALEGKKGFCPRDDSELLRVYSSINRSVIIDACADCRGIWLD
                     GGEFEKLFSARQL"
     gene            3986..6604
                     /locus_tag="VF182_15140"
     CDS             3986..6604
                     /locus_tag="VF182_15140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6438472.1"
                     /translation="MPLVNMPLPQFSEQFPESLESLPLSSRHDSTPLNGSEKITAQGR
                     FFFNNHEKFFLKGVTYGPFAPSLHGDPFPEAARVETDFTLMRKLGVNCFRTFTPPPDW
                     LLDMAGASGLRVIVGIPWAQHISFLDSPRTKSEVRKTIVGSVEACKRHPATFAYLIGN
                     EIPPDIVRWHGAQRMRSFLGSLFKAAKASDPDRFVSYANFPSTEYLEIDFTDFLAFNV
                     YLHREQDFRRYLFHLHNLAGDRPLVLTEIGIDSVSQGLNFQAQAMSWQLRVSFETGVA
                     GTVIFSWTDDWHAYSGADGFQVDDWAFGLVDRNRRPKPAYWEAKKYYVNTLPAALNEY
                     PKVSVVVCVYNGERTINACLASLEKLNYKNYEVIVVNDGSTDRTQEIAESYGYVRLIN
                     QENKGLSEARNVGIQAATGDIIAFTDADCMADEDWLSYLVARFQSSEFGAVGGPNLAP
                     PDDSKIAACVALSPGAPAHVLLDDEIAEHIPGCNMAFRREALEAISGFDPIFRAAGDD
                     VDVCWRLQNKGYKIGFSPAALVWHFRRNTVRDYLKQQRGYGKAETLLFFKHPSRFNVL
                     GQSRWFGRIYGDLSSFLSSRQPRIYSGVFGRGLFQTLYQPPPSVLACLPMTLEWNLLS
                     LALIACAFIFGGWFWLGLAPFGITVARCLASALRARVPRAYRGGAATLLITFLIFVGP
                     FFRTLERYRWRIRRLREVKRVECNGPRQSPRISLRERAFYLSYWNEAGQEKESLLGAA
                     MDFLVPRKYLIAMDQGWSQWDLEICQGPWAKAQVKTAVENHGGSKRLLRVRCALRMSR
                     VSLICLSLYAVLTTTAAFLATPQIALATLAIGLIHGGAILYQKFRLGHVLYHVMESLA
                     HKLEFVPVAKSSPEVL"
     gene            6633..8414
                     /locus_tag="VF182_15145"
     CDS             6633..8414
                     /locus_tag="VF182_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6438473.1"
                     /translation="MKRTVRSYLKPYWPAFVLALGQVFLISALELLKPWPLKIIIDNI
                     LSDNPLPLGFARDWSSETLLIAACAGLVVIYILLGGVRFLNDCATVRIGQGMVNDLRR
                     DLYSRIQRLSLSFHTRQQVGDLMYRITADTMCIQALTMNGFFSVLSASVLLAGMFVVM
                     LSLDTSLTLLALAVCPALFVAIGLLNRKMSSAAAHARQKESLVYSLLQRTLSGIRIIQ
                     AFTKEEDEERRFVKASTESLAADLRLYNLQNFYFAVVNVTIAAGTAAVLWVGTRHVLS
                     GQLSVGDMVVFTSYLASLYVPLNNIFETYGLVQSAKVGVRRAFDILDTEQSMPEGHRN
                     LGKHRAKGEISFEQVSFGYDSARAILKHIDFRVAPGQKIAIVGPTGAGKSTLVSLLPR
                     FYDPQSGRITIDGVDIREFRLKFLRRNIAMVLQPPLVFPFSIRENIAYGRPEASLEDV
                     ISAAKVAQVHDFVEQLPDKYETMISEQGFTLSEGQKLRLTIARGILLDAPILILDEPT
                     SSVDSETETLIMDGLEHLMDGKTTFIIAHRLSTVRQADLILVVRAGQIVEQGTFGELL
                     HRGGPFASLYRTQFGLRDDERKFRLVK"
     gene            8425..9150
                     /locus_tag="VF182_15150"
     CDS             8425..9150
                     /locus_tag="VF182_15150"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6438474.1"
                     /translation="MPFIPDFTDPRYVDEVGWFLYHEKYGRDEFGGSYDAERRAYSRL
                     LLEEVAKYLKQEPTWLEDKTVVSIGCGCSGDLVSFPSRLKIAIDPLLCVYQQLGLLMA
                     DEAGSATVYLSIAAEELPLLSDYADLVICRNALDHMHNPAVALREMRRILNNEGSLFL
                     SVDLGGEPTPDEPTVFSADSLEALLLQEQFEIVDSVRDEQPHSAWRPSSTRIMVRKKP
                     QNALILDKQEILRRYVARLGDHQ"
     gene            9188..>9681
                     /locus_tag="VF182_15155"
     CDS             9188..>9681
                     /locus_tag="VF182_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438475.1"
                     /translation="MESKLRIIVTGLIAQYPLGGVTWDYFQYVLGLAQLGHDVYYLED
                     TGQWPYNPQEGGLGKGCDFNVQYLAAVMSRYGLTEQWAYRFPWQSQWFGLDDAKRQEV
                     IRSADLLINVSGTLERPEEYRQVSRLAYIDSDPVFTQIKLARGQLDFRKWIDAHDVQL
                     SFGE"
BASE COUNT         1968 a         2640 c         2594 g         2479 t
ORIGIN      
        1 atgcggcttc tcgggatact aactggcggg catttcgtga tccattggtt tcagcagttt
       61 tttcctgtca ttctaccttc gatcaaatcg ggtctgtcat tgactaatat agaagtcggc
      121 gctttgacgt cggcgcagca gatgatcatg ggcttgggcc agctccccgt gggcttgctc
      181 gccgattcaa tggtacgcca tcgcgccaag atgctgggac tgtcgttggt agcgatgggc
      241 gctgcctatt ttctcttggg atctccgggt tttgtctggg ccctactcgg ctcggggttg
      301 atcggtttag gcacggcgct ctggcatccg accgctgctg catcgttgtc gaatcgtttt
      361 ccggaacatc gtgcaaccgc cctctcgatt cacggcacag gagcaaccat cagcgatacc
      421 ctcacgccgc tgtttgtcgg cgcgttgctg gcgagtttct cttggcagaa cgtcgcacgc
      481 ctgcaattgt tgccaggact gttgttcgcg gtgttggtct ggcgcgctct tgccgaggcc
      541 ttcagcgatg gcgacccgcg tccacgccaa ggatcaactc aaatccgcga ggtgatgggc
      601 gtggtccgaa accccgcttt cctcggagta tcggcagcca cggggttgct ctcgatgggc
      661 cgtttggtga ttttgacttt tctgcccatc tatcttcagg aacacctgca ttattcagcc
      721 ttcgcgctgg gagttttcgt cgccttgatg cacgctctcg gcacaatttc gcaacccgcc
      781 ttgggccttt tgtccgatcg ctttggccgc aaaaccgtgc tgatgccttc atgcctattg
      841 ctgggtttgt tctttgcgct gctggcggtc gtacccgcgg gaattcctct ggcgttagtg
      901 atcattgcca tcggtctttt tttctacacc cttttcaaca tcatgaatgc cgcggtaatg
      961 gatgtcgccg gggccgcggt acaggcgtcg acctacggac tcatgacact catcacgcag
     1021 ctggtggtga ttccgacgcc gatggttacc ggatatttga tcggcgaatt tggcatcaag
     1081 ttctcgtttg ttctggcggg agtttttctt atcatcgccg gcctggtact cgcgccattg
     1141 aaactctacc gcggatcgcg cgcttgatat gcgctgaatg cagaaagtct tgcgcgccaa
     1201 ctctgagagc ttttgatttg gactctgctg catcggcggg aatttcaggc ggcgattctt
     1261 cgcatcgtgt ttgacgagcc cctccatcgt ctggcataaa acataagacg ccttttctcc
     1321 gccatgaaac tttcccgcgc caaggtttta ctgctctcca cggctgtcgt cactatgact
     1381 tccatcggga tgagctcgtt tagtctgttc cttaggccca tggaagcgga tttcggttgg
     1441 tcgcggtcaa tggtgaccct gccttatatg tttgccatgc tcggttgggg cgccggcgcg
     1501 gttctgttcg gcaagctcgc cgacgattac ggcgcgcgac gggtgattct gggcggcatc
     1561 ctgctcatgg ccagtggatt tttcggtatg agcctgtcga gaaatgtctg gcaactttca
     1621 ctcacgtatg gcctgatggt gggcatagct atgggcgcat gcgggctggc aatcgcgtcg
     1681 cttttggttt cgcaacacta cgagtccaag agtcgcggcc tggcagtgag cgtgctgcaa
     1741 acttcggctc cgctaagccc gcttcttttc gcggcgccgc tttatttttt gatcaaccgg
     1801 tttggctggc gcgccggcgc tttatcgaca gcggtcctgc tcattgctgt ggcattaccc
     1861 ctggcatggc tgggcgcacg cgagccgcat ggttccggcg tccgacgggc gcgcctcggc
     1921 tggagggcgt gtcttcccta cctgcgcaac cgttccgtgc tggtcatgtt cgcagcgcgt
     1981 ttttcctgcg gcgtggcttt ctttcagatc gctcatctgg tggcgctggc attgagcaag
     2041 ggcttcacca acgccaccgg cgccgccgca gtcagcgtat tcggagccgc ggcggcggca
     2101 tcggcgctgc tcttcggctg gctctccgac cgctacggca gggctcagat gctcgctctt
     2161 agctatatcg tgcgtggggt tggcacattt ttcttatcgt ttgacgttcc caacgagctg
     2221 gtatttttca tcctcgttgc tgtggccatc ggtccgacct ttggcaccgt ggctgttcaa
     2281 aatgtaatgt tttatgaaat cgccgggccg cgacttgccg gagtcattct cggccttagc
     2341 ttcattgtgc accaaatcgg ctcggcgggc ggacccatgt tcgccagtgt cgtctttgat
     2401 cgaaccggca gctacgacgg cttcatggcc gtgatggccc taattctgat cgccgccggc
     2461 ggactggtct acagcaacat caaccagcag acgcagtttc ccgaattggt ttttgggtcg
     2521 agcccggcac gctcgtaaaa tcaattgcgc gcgcttacgg atctgcactg cggatcatca
     2581 atcttgctga tcaggccgga caggagcccg gcgcgatttc ttttcagcca gatggcgctt
     2641 atcggcgagc agcttgagct tggctcgccg cgagcgcttt cttttttgac gccggatctt
     2701 agcaatctct tgctcttcag cggattgcgc tcccttgatc tttgactcga ttttatcaag
     2761 caggatgcga cgggcaagaa atcgattaag ggcttgcgaa cgttcctgct ggcacttgac
     2821 ccgtataccg gtgggccggt gatgaagaag gacgcacgag gataccttgt tgacattctg
     2881 gccgcctgct cccgacgagc gaacaaattg ttcttccagg tcctgctctc ggacacctaa
     2941 ggctcgcatt cgagattgca gtagtttctc tttttcggaa gagaccggat ttcctgacat
     3001 gatcgagctc cgggaaacgc gggaaagaac taaagctggc gagcagaaaa gagtttttca
     3061 aattcgccgc catcgagcca gatacctcgg caatcggcgc aggcatcgat aatcacggat
     3121 cgattgatac tgctgtagac tcgtaaaagt tctgagtcat cgcgcgggca gaagcctttt
     3181 ttgccttcca gcgctttggt atcgcgtccc cgccgcagcg aagataaatg gcgagcatct
     3241 tgttcaagca gttggctgag ctcccgcgcg tcgaaccaaa ttccgctgca agacgagcag
     3301 cgctccaagg ccactccttc taccgaaaat acattcatag tctcagtacg gcattttgga
     3361 catctcattg cactcatgcc tcatcctttc atcttttgtg gaacagtaaa atcgtaaatg
     3421 gtaggccgga aactcgcgaa agattaatct aagaaggcta cccattcgaa tacaaaatcg
     3481 tatgcgtttg cgtgttaatg caatttattc tcgtcatctg aatgatcgga tcaacgtcta
     3541 caaaataata tagctaattt tccgcatcga cgaattcggt cagcgacgcc acccatcgag
     3601 gcggtgacct aacatctgtg tttacaacgc tcgcgtttct gtgcgtcttc cctttatatc
     3661 attgcaccga caacgaatct gaagcttgaa gccctctcca tcgtcaaaga ctacaacggt
     3721 ggtctgagtc gggggacccg cattgaccaa cagaggtgta gtccggtgca ggataaacct
     3781 gcagtgaagt gcggccgcca gacccaaagt atgctctcaa ggtaatatag acgccgttta
     3841 aagtaccata aatcaagaag attccttttt gggagtttac tggcattgtt gttgcgttgc
     3901 gcaccattgc tactcatata gccttttcgt attctttcgt ctaaatgatg cggtttgatt
     3961 ttctatagga gaaacaccag tgaatatgcc gctagtgaat atgccgctgc cgcaattttc
     4021 cgaacagttt cctgaatcgt tggaatctct gccactatcg agccgtcacg actccacgcc
     4081 gctaaacggt tcggaaaaga tcaccgcgca aggacgtttt ttcttcaaca atcatgaaaa
     4141 gtttttcttg aaaggagtca cctacggccc ttttgctcct tcacttcatg gtgatccatt
     4201 tccggaagca gcacgtgtcg agaccgattt tacattgatg aggaagctcg gagtcaactg
     4261 cttccgcacc tttacgccgc cgcccgactg gcttctcgat atggccggag cctccggcct
     4321 gagggttatc gtcggcattc cttgggctca acatatatct tttcttgatt cacctcgcac
     4381 gaagtctgaa gttcgtaaaa ccattgtcgg cagcgttgag gcctgcaagc gacatcccgc
     4441 gacgttcgcc tatttgatcg gcaatgagat tcccccggac atcgtacgct ggcacggcgc
     4501 gcaacggatg cgatcgttcc tcggcagctt attcaaagca gcgaaagcaa gcgatccgga
     4561 ccggttcgtg agttacgcca attttccttc tactgaatac ttggagatag attttaccga
     4621 ctttttggct ttcaacgtct atcttcaccg cgaacaggat ttccgccggt acctttttca
     4681 ccttcacaat ttagccggcg atcggccgtt ggtgctgacg gagattggca ttgactcggt
     4741 gagccaaggg ctcaactttc aggcccaagc aatgtcctgg caattgcgcg tgagcttcga
     4801 aacaggcgtt gccggcaccg tgatcttctc atggaccgat gattggcatg cgtattcggg
     4861 cgctgacgga tttcaggttg acgattgggc tttcggttta gttgatcgta accggcggcc
     4921 caaaccagct tactgggaag caaaaaagta ctacgttaat acgctcccgg cggctctcaa
     4981 cgaatatccc aaagtttctg tggtggtatg cgtgtataac ggcgaacgca caataaacgc
     5041 ctgcctcgcc tcattagaaa agctcaacta caaaaattat gaagttattg tagtaaacga
     5101 tggctccact gatcgaacac aggaaatcgc cgaaagctat ggctacgttc gtctcatcaa
     5161 ccaggaaaac aaaggactca gcgaggcgcg caacgtcggt attcaggctg ccacaggcga
     5221 tatcattgcc tttaccgatg ccgattgtat ggccgatgaa gattggctga gctatctggt
     5281 cgcccgtttt caatcgtccg aattcggcgc tgtcggcggc ccgaatctcg ctccgcccga
     5341 cgattcaaag atcgccgcat gcgtcgcctt atcgcctggc gctccggccc atgtgctttt
     5401 ggacgatgag atagccgaac acatacccgg atgcaatatg gcattccggc gggaagcgtt
     5461 ggaagcaatc tcagggttcg atccgatttt ccgcgccgcc ggcgacgatg tggatgtttg
     5521 ctggcggctt cagaataaag gttacaagat cggcttcagc ccggcggcgc tggtgtggca
     5581 ttttcgccgc aatacggtgc gagattactt aaaacagcaa cgcggctatg gcaaggccga
     5641 gactttgcta ttcttcaaac atccaagtcg tttcaacgtg cttggtcaat cgcgttggtt
     5701 cggcagaatc tacggcgatc tctcgtcttt cctgtcgagc cgccagccga ggatttactc
     5761 cggggtattc ggacgtggac tttttcaaac gctctaccaa ccaccgcctt ccgtcctagc
     5821 atgtctaccg atgactcttg aatggaactt gctttctctc gcactcatag cgtgcgcgtt
     5881 tatttttggc ggctggttct ggcttggact cgcgccattc ggcattacgg tagcgcgatg
     5941 cctggcaagc gcgctacgcg ctcgcgtccc ccgagcttac cggggcgggg cggcaactct
     6001 gctgataaca tttctcattt tcgtgggccc attctttcga actctcgagc gctaccgctg
     6061 gcgcattcgc agactgcgcg aggtcaagcg ggtcgagtgc aacggcccgc gacagtcgcc
     6121 gcgcatctcg ctgcgcgaac gggcctttta tctgtcgtac tggaacgagg caggccagga
     6181 aaaagaaagc ctgctcggcg ccgcaatgga ctttcttgtg ccgcgaaagt atctcatcgc
     6241 catggatcaa ggatggagcc aatgggatct ggaaatttgc caagggccgt gggcaaaagc
     6301 tcaagtcaaa accgcggtgg aaaatcatgg cggctcaaag cggcttttgc gtgtgcgctg
     6361 cgctcttagg atgtcaaggg tttcgctgat ctgcctttcg ctctacgcgg tattgacaac
     6421 gaccgccgct ttcctggcga cgccgcagat tgcactggcg acacttgcta tcgggctgat
     6481 tcacgggggc gcgatccttt atcaaaaatt ccgcctcggc cacgtgctct atcatgtcat
     6541 ggaatcttta gcgcacaaac tcgagttcgt gcctgtcgca aagtcatcgc cagaagtgct
     6601 gtgacagaat ccgccctctt taggcaaccc ttatgaagcg cacggttcgt tcctacctaa
     6661 agccgtattg gccggccttc gttctagccc tcggtcaggt tttcctcatc agcgcgttgg
     6721 agcttctcaa gccatggccg ctgaaaatca ttatcgacaa tattttgagc gacaatccct
     6781 tgccgttggg attcgctcgc gactggtctt cagagaccct tcttatagcc gcttgcgccg
     6841 gcttagtcgt tatttacatc ctgctgggcg gtgtgcgctt cttgaatgat tgcgcaacag
     6901 tccgaattgg ccaaggcatg gttaacgacc ttcgtcgaga cctgtacagc cggattcagc
     6961 gcctgtcgct ctcctttcat acccgccagc aggtcggcga ccttatgtat cggattaccg
     7021 ccgacactat gtgcattcag gcgcttacca tgaatgggtt tttctcagtc ctctcagcct
     7081 cagtgctgct ggcaggaatg ttcgtggtaa tgctctcgct cgatacctct ctcactttac
     7141 tggctttggc tgtgtgcccg gcactcttcg ttgccatcgg gcttctcaat aggaagatgt
     7201 cctcggcagc tgcgcatgct cgtcaaaagg agagtttagt ctattcgctc ctgcagcgga
     7261 cgctctcagg aatacgcatt atccaggctt ttaccaaaga agaggacgag gagcggcgtt
     7321 ttgtcaaagc aagcaccgag agtctggcag ccgatttgcg gctctacaac ctacaaaatt
     7381 tctattttgc cgtcgtaaat gtcacgatcg ctgccggaac tgccgcggtt ctgtgggtag
     7441 gcacccgcca cgttctatca ggacagcttt cggttggcga tatggttgta tttacctcgt
     7501 atcttgcttc cctctacgtc ccgctgaaca acatcttcga aacttatggg ctggtgcaga
     7561 gcgcaaaagt tggggtccgg cgagcgtttg acattctgga cacggaacag agtatgcccg
     7621 aaggccacag gaatcttgga aaacaccggg cgaaaggcga gatctcgttt gaacaagttt
     7681 cgtttggata cgactctgcc cgagccattt taaaacatat cgattttcgc gtcgcgccgg
     7741 gtcaaaagat tgcgattgtc ggcccgaccg gcgccgggaa gtccacgttg gtgagcctcc
     7801 tgccgagatt ttacgacccg caatcgggac ggataactat cgatggtgtc gacatacgcg
     7861 aatttcgatt gaaatttctg cgtcgcaata tagctatggt tctccaaccg ccgctggtct
     7921 ttccctttag catccgtgaa aacatcgctt atggtcgacc ggaggcgtct ctggaagacg
     7981 tcatcagcgc ggctaaggtg gctcaggttc atgatttcgt cgagcaactt cccgacaagt
     8041 acgaaactat gatcagcgaa caaggattca ctttatccga gggacagaaa ttgcgtctca
     8101 ccatagcgcg cggcatctta cttgatgcgc ctatactgat tctggacgag cccacttctt
     8161 ccgtggattc ggaaaccgaa acgcttatta tggatgggct ggagcatctg atggacggca
     8221 agaccacttt tattatcgct caccggctct ccacggttcg acaagcggat ctcattctcg
     8281 tcgtccgcgc cggccagatt gttgagcagg gcacgtttgg cgagctgctg catcgcggtg
     8341 gcccgtttgc gtctctgtac aggactcagt ttggcttacg cgacgatgag cggaaattcc
     8401 ggctggtcaa ataggacaga gcacgtgcca ttcataccgg actttacaga tccacgatat
     8461 gtagatgaag ttgggtggtt cctctatcac gagaaatacg gtcgtgatga gttcggcggc
     8521 tcttatgacg ccgagcggcg cgcgtactcc cgacttttgc ttgaagaggt cgctaagtac
     8581 ttaaagcaag agccaacctg gctggaagac aaaaccgtcg ttagcatcgg ctgcggctgt
     8641 agcggtgacc tggttagttt tccttcccga ctcaaaattg caatagaccc gcttctttgt
     8701 gtttaccaac agttggggtt gctaatggcg gacgaagccg gtagcgcgac tgtttatttg
     8761 tcgatagccg ctgaggagct tccgttacta tctgattatg ccgacttggt gatctgtcgg
     8821 aacgctctcg accacatgca taatcccgca gtggcgttaa gagaaatgag gcggattctc
     8881 aacaacgaag gatcattatt cttaagcgtt gatcttggcg gcgaacccac cccggatgaa
     8941 ccaaccgtgt tctcagccga cagccttgaa gctcttcttc tacaggagca gtttgagatc
     9001 gttgattctg tccgcgacga acagccgcat agcgcgtggc gtcctagcag cacgcgcatc
     9061 atggtgcgga aaaaaccaca aaatgcgttg atcctcgaca agcaagagat cctccggcga
     9121 tatgtagcac ggctcggcga ccaccaataa tggcagtcat cgagcgcggg cgcagaggaa
     9181 accagccgtg gaatcgaaac ttcggatcat tgtcacaggg ctcattgctc agtatcctct
     9241 cggtggcgtg acttgggatt actttcagta tgtactcggc ctcgctcagc tgggtcatga
     9301 tgtatactat cttgaggata ccgggcaatg gccttacaac ccgcaagaag gcgggctcgg
     9361 caaaggctgt gacttcaatg ttcaatatct cgctgccgtt atgtcgcgct atggtctcac
     9421 ggagcagtgg gcctatcgct ttccgtggca atcgcagtgg tttgggcttg acgacgcgaa
     9481 acgacaagag gtcattcgtt ccgctgactt gcttatcaat gtttctggga ccctagaacg
     9541 acccgaagag tatcgacagg taagccgcct tgcgtatatc gattcggacc cggtctttac
     9601 gcagattaag ctagcgcgcg gacagctcga tttccgtaaa tggatcgacg cgcacgacgt
     9661 ccagctcagt ttcggggaat g
//
LOCUS       DASUVS010000276         7616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1644057, whole genome shotgun sequence.
ACCESSION   DASUVS010000276 DASUVS010000000
VERSION     DASUVS010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644057"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..1572)
                     /locus_tag="VF182_15160"
     CDS             complement(265..1572)
                     /locus_tag="VF182_15160"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HEX6438476.1"
                     /translation="MKLELSEFPVKQLRLGHRFTYGNGTLEMDEGELLDLVLQDSRIT
                     EAWLSVAMPGEKTRITAIRDIVEPRIKVSGNGRVFPGVLGPVTDVGEGTTHRLSGMTV
                     IAAAAYEGTIRAGTTVQRSAILDMSGPAAEISRFSAHAHLVVSFRLAPGLGELDAHTA
                     IQLAEYKVAQRLAQVTIGLSPESVRSFDLSMETPGLPRVMLVQGCITDPQHVHSGVGY
                     YGLSLRQSMATFLHPNELFDGAITVDTTRSGRGYYPTTWDWQNHPLVLRLYEAHGKDI
                     NFAGVILQRIRFETAHGKEVGAQNAARLAKMFRADGALLTWIGGGNAFVDVMLTVQAC
                     ERNGIKTALVTYEHGGKEGRDSPLLFYLPEADAVVTTGSLDRAVELPAMDKVIGPYEE
                     IKVLSYPGVPASPARGALSLEGRDVIIGGGDIWGEQRWVCSDY"
     gene            complement(1619..2692)
                     /locus_tag="VF182_15165"
     CDS             complement(1619..2692)
                     /locus_tag="VF182_15165"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6438477.1"
                     /translation="MRKIIKVMMCLALCQSLMWFSVFARVDAATIAEVTAKVSSLNPQ
                     DKQNVLVKGAQEEGELVYYGTILVNEFTELGKSFKARYPFLNLKHYYAPREGILNRSL
                     TEARAGSYAVDAIQVDSSYGYQLINENLVHPYAVGARDRFYDGTYDPSGSWHSMYYLT
                     TAMIYNTTLVKPESVPQTYDTLLDPSWKGKLLFDPEAGYILAALEQAWGREKAVDYLT
                     KLRKQDLSFRRGGALTTQVVSSGEYPIGIAINGETSAAIRDKGAPLGFKVLSPKIVKP
                     EGLFLARNSPHPHAALLFAEWVLSEEGQTVLAGKLGKGIAMKGVRSKFQEFQVQPDYV
                     VTPKLGPNLKTYIEDFARIVGAR"
     gene            complement(2810..4135)
                     /locus_tag="VF182_15170"
     CDS             complement(2810..4135)
                     /locus_tag="VF182_15170"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HEX6438478.1"
                     /translation="MSSLANRMRLNLAEFPVKEIQLVKRFHYRTGILELNQHALVDLV
                     LDDRRIEEASFAVVHPGEKVRVTGIRDIVEPRVKVRSQGQVFPGALNPVEHIGDGTTH
                     RLSGMAVIATAAYEGTVRAGLAVQRSALLDMWGPGADASRFSSMIGLVLVMRLKQELS
                     DLDAHQAIQGAELKVAQRLAETTIGEQAESTEVLDLSDAHPNLPAVVLIQGCLTDSHN
                     VHSGVSYYGLPIRDSLATFVHPNELLDGAITVTATRAVGHFPITWDWQNHPLALGLCR
                     QHGKQINFLGVILERIQFDTFLGKEVAAHSASQLAATLGAAGALVAWIGSGNAFVELM
                     LTIRACEQRGIETVLITYEFGGKDGVDSPLLYYVRDADAVVSSGSRDRWLELPAPQRV
                     VGPYQEFSILTYPGAPVVPAMGKITLDARDMLIGGVDNWGSQSWRCNAY"
     gene            complement(4119..5105)
                     /locus_tag="VF182_15175"
     CDS             complement(4119..5105)
                     /locus_tag="VF182_15175"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438479.1"
                     /translation="MRLRISFTVALLLVASYVQAATQTVISYASFNERTAGALLVAQD
                     QGFFRKQDLDTRLVYVRAGNVALSALAAGESQFYFGSGTGVTLGAVAGGLDGVFVAGL
                     VNRLTGTFVVQPQIKTPADLKGRNIGVQSIGGGIWMITMLVLDHWGIEPKRDGINLRI
                     VGDEAVLAQALATNLIEGTYLGYTFGSLMKRQGFNVLADLASLKIPYQSTGVFVRRSF
                     LKSSPDTVEKVLRAMTQATAYIAKPENKNSVMTSLYKGLRLPRFEDAEEGYELMRTLY
                     ERRIYPNTDGIRNAIRLLGSSNEKIRQLKAENMVDDRLVRKLEREGAFEQPR"
     gene            complement(5434..6879)
                     /locus_tag="VF182_15180"
     CDS             complement(5434..6879)
                     /locus_tag="VF182_15180"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6438480.1"
                     /translation="MPKTLRTFLDDMRRIYPNEVVTISKTVNPISYDVTAIVKQLGAL
                     KRFPILIFDNPLNPHGELNGIRLAIGTENTQKKVQVALGVRAEMDRAEMAREILRREA
                     GRIAPILVDRRAAPIKEVIQKGDEVDLYQLPLMRHHEMDGGPYIDMSSVARDRHSGVY
                     NASYHRMEVKDRNHTGFLMTLQHLWRIFRGYEEANEECPVATVIGHHPGYQMGACYGG
                     PFEISEYEIIGGYLQEPLRLTPSETWGERLLVPADAEIVIEGALLPGKRMVEGPFGEV
                     NGYLGMQGFQQSACYEVRAITRRRDAMHMSILTPEGDKPWMDLAREGAYLRRCREAVP
                     NVQTVCTSGRHALLNVFISMKKMSEGDPGRAAAAVLTWDWCKNVFVFDEDIDVYNPTE
                     ILWALATRVQPHRQVSIIGEIMRASLIDPSLESPRKTSAMIVDATRPLDRPFSPVSKC
                     PDEALARIRLEEYVPGEVLQHIPVDRTTYWA"
     gene            complement(7109..>7616)
                     /locus_tag="VF182_15185"
     CDS             complement(7109..>7616)
                     /locus_tag="VF182_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438481.1"
                     /translation="PNDTNMGEMLMQGRLDGALRYLAGTDNLVDRSTVDLYRHPDFKT
                     LFPDPHAEGVRFYQKTGIYPINHCMVIKREIVDRHPWTVLNLFKAFVRANDIAEKARM
                     EAVAYHLETGLLPPEARKALATPLIRHGIAANRKVLETTAQYSFEQGLTPRLVKIDEM
                     FAPSVMEQ"
BASE COUNT         1638 a         2279 c         2042 g         1657 t
ORIGIN      
        1 aatctggaac cgtccagatt gctgccaggg gcgaccgatg gaatcagcac cgcttatgag
       61 acgccgcggc cagaaggctt gctccctcgc tccagtttcg caatcatcca ctctagcagt
      121 atgctgaaaa ttctcatgca agatgccaaa agatctcaga agacgtaagg cgcgcgaagt
      181 tcgacaagct gaggcttacg cttaagttac gtcgaagcga gtcgcgcgag cgcaccgaca
      241 ctatgagtcc ttatcagcag cttgctagta atcactgcaa acccatctct gctcccccca
      301 aatgtcgcct ccaccgataa tcacatctct tccttccagc gagagagcgc cacgcgccgg
      361 actcgcaggc acgccgggat aggacaatac tttgatctcc tcgtacggac cgatcacttt
      421 gtccatcgcc ggcagctcca cggcgcgatc gaggcttccg gtcgtgacca cggcatcggc
      481 ttccggcagg tagaaaagca gcgggctgtc gcggccttcc ttgccgccgt gctcgtaggt
      541 caccaacgcg gttttgattc cgttacgctc gcacgcctgc acggtcaaca tcacatcgac
      601 aaaagcattg ccgccgccga tccaggtcag cagcgcccca tcggcgcgga acatcttcgc
      661 cagtcgcgcc gcattctgcg cgccgacctc tttgccgtgc gcggtctcga aacggatgcg
      721 ttgcaaaatc acaccggcga agttgatatc ttttccatgg gcttcgtaca gccgcaaaac
      781 cagcggatgg ttctgccaat cccacgtagt cggataataa ccgcggcctg accgggtcgt
      841 gtccaccgtg atcgcgccgt cgaacagctc gttggggtgg aggaaggtcg ccatcgattg
      901 gcgtagcgat aggccgtaat aaccaactcc tgagtgcaca tgttgcggat cggtgatgca
      961 gccttgaacc agcatcactc ggggcagccc gggagtttcc atgctcaaat cgaagctgcg
     1021 aaccgattca ggcgaaagtc cgatagtcac ttgagccaga cgttgagcga ctttgtactc
     1081 ggccaactga atcgcggtat gcgcatccag ttctcccagg ccaggcgcga ggcgaaagct
     1141 cacaactaaa tgcgcgtgcg cgctgaagcg cgagatctcc gccgccggtc cgctcatgtc
     1201 caggatcgcg ctgcgctgca cggtagtgcc ggcgcggatg gtaccttcgt acgccgccgc
     1261 cgcaatcacc gtcattcccg aaagccggtg ggtcgtcccc tctccgacat ccgtaaccgg
     1321 ccctagaacg ccgggaaaga cgcggccgtt tcccgaaacc ttgatgcgcg gttcgacgat
     1381 atcgcgaatg gcggtgatgc gggttttctc ccccggcatc gccacggaga gccaagcctc
     1441 ggtaatccgc gaatcctgga ggactaggtc gagcaattcg ccttcgtcca tttccaacgt
     1501 gccgttcccg tatgtgaaac gatgtcctag gcgtaattgc ttgaccggga attcggaaag
     1561 ttcgagtttc ataggttgga tttcattacc ggttcgcctg gaaaatcgtt aatcccagct
     1621 atcgcgcgcc gactatcctg gcaaaatcct cgatataggt tttgagattg ggtccgagtt
     1681 tgggagtcac cacgtagtcc ggctgcactt gaaattcctg aaatttgctg cgcacgcctt
     1741 tcatcgcgat ccccttcccg agcttgcctg ctaacactgt ttgcccttcc tccgaaagga
     1801 cccattcggc gaaaagtaac gccgcatgcg gatgaggtga gttcctcgcg agaaacaaac
     1861 cctcgggctt gacgatcttg ggcgataata ctttgaatcc caacggcgcc cccttatcgc
     1921 gtatggctgc ggaggtttcg ccattgatcg caatgccgat gggatactcg cctgaactta
     1981 ccacctgagt agtcaaagcg ccgccgcgcc tgaagctgag atcctgcttg cgcagcttcg
     2041 tcaaatagtc cacggccttt tcccgtcccc atgcctgctc cagcgctgcc agaatatagc
     2101 ccgcctcggg atcgaacaat aatttgcctt tccacgacgg atcgagaagc gtgtcataag
     2161 tttgcggcac cgactccggc ttgaccaaag ttgtgttgta aatcatcgcg gtggtcagat
     2221 aatacatgct gtgccatgag ccgctcggat cgtaggtgcc atcatagaat cgatcccgcg
     2281 cgccaacagc gtaagggtgg accaaatttt cattgatgag ctggtaacca tagctcgaat
     2341 ccacctggat tgcgtccacg gcgtagctgc cggcgcgcgc ctcggtgagc gagcggttaa
     2401 gaatgccctc gcgtggcgca tagtagtgct ttagattcaa gaacggatag cgcgccttaa
     2461 aagacttgcc gagctcggta aattcattga ctaaaatagt tccgtaataa accagctcgc
     2521 cttcctcttg agctcctttg acgagaacat tctgtttgtc ctgaggattg agggagctga
     2581 ctttcgccgt cacctccgca atcgtcgcag cgtcaacgcg cgcgaaaacc gaaaaccaca
     2641 tcaatgactg acatagcgcc aagcacatca tgaccttgat gattttacgc acccgtctaa
     2701 cctgcctttc tcctcgttat tatcgtccgc catcattcaa gcttcatccg cacacgcctc
     2761 agtcagaaat ccgttgatgc tcgcatcggt tgaactgcag aacgacggtt tagtaagcat
     2821 tgcatcgcca gctttgactg ccccaattgt cgacgccgcc gatcagcatg tcgcgcgcat
     2881 ccagggttat tttccccatc gccggcacga ccggcgcgcc ggggtaagtc aaaatcgaaa
     2941 attcctggta gggtccgacc acacgttggg gcgcgggaag ttcgagccag cgatctctac
     3001 tgccgctgct cacgaccgca tcggcgtctc tcacgtaata aagcaacggt gaatccacgc
     3061 catctttacc gccgaactca taggtgatca gcacggtttc gatgccacgc tgttcgcaag
     3121 ctcgaatggt cagcatcaat tccacgaagg cattgccgga accgatccat gccaccagag
     3181 cgccggcagc tccgagggtc gctgccagct gagacgcgct gtgcgccgcc acctctttgc
     3241 ccaggaaagt gtcgaactga atgcgttcga gtatcacgcc gagaaaattt atctgcttac
     3301 cgtgctggcg gcacaatcct agtgccagcg gatgattctg ccagtcccag gtaatcggga
     3361 aatgaccgac cgcgcgagta gcggtgacgg tgatggcgcc gtcgagtagt tcgttggggt
     3421 gtacaaacgt cgccagagaa tcgcgaatcg gtaggccata gtaactcacg cccgagtgaa
     3481 cgttatggct atccgtgaga caaccttgaa tcaacaccac ggccggcaag ttcggatgtg
     3541 catcgcttag atcaagaact tcggtgcttt cggcctgctc gccaatggta gtttccgcaa
     3601 gtcgctgcgc cacctttagc tccgcgcctt ggatggcttg atgcgcatcg agatcggaga
     3661 gctcttgttt aagccgcatc accagcacca gcccgatcat cgagctaaac cgcgaagcat
     3721 cggcgccagg tccccacata tcgagcagcg cgctcctctg caccgccagg ccggcgcgga
     3781 ccgttccctc ataggcagcg gtggcgatca ccgccattcc ggagagacga tgcgttgtcc
     3841 catcgccgat atgctctacc gggtttaacg cccccggaaa cacctgaccc tgagaacgca
     3901 ctttcacccg cggctcgaca atgtctcgaa tgcctgtcac gcggactttc tcccccggat
     3961 gaaccacggc gaaggacgcc tcttcgatgc gccggtcatc caagacaaga tccactagcg
     4021 cgtgctgatt cagttcgaga atcccagtcc ggtaatgaaa tctttttact agctgaatct
     4081 ctttcaccgg gaattcggct agattcagcc gcattcgatt agcgaggctg ctcaaatgcc
     4141 ccctcccttt ctaacttgcg gacgagccga tcgtcgacca tgttctcggc tttgagctgg
     4201 cgaattttct cattgctgct tccgagcagg cggatggcat tgcgaatgcc atcggtgttc
     4261 ggataaatgc gccgctcata gagcgttcgc atgagctcat agccttcttc ggcatcctcg
     4321 aaacgcggca agcgcaatcc tttatagaga ctggtcatga cactgttctt gttttccggc
     4381 ttcgcgatgt atgccgtggc ttgcgtcatg gcccggagca ccttctccac cgtgtccggt
     4441 gaggatttaa gaaagctacg ccgcacaaaa acgccagtgc tttgatacgg aattttcaag
     4501 ctggccagat cggcgagcac attgaatccc tgacgcttca tcagggatcc gaaggtatag
     4561 cccaggtagg taccctcgat caggttggtg gcgagcgcct gggccagaac cgcttcgtcg
     4621 ccgacaatcc ttagattgat gccgtcccgt ttgggctcga tgccccagtg atccaagacg
     4681 agcattgtga tcatccagat gccgccgccg atgctttgca cgccgatgtt ccggcctttc
     4741 agatctgcgg gagttttgat ctgtggctga acgacaaaag tacccgtcag ccgattgacc
     4801 aagccggcga caaagacacc atccaagccg ccggccaccg cgccaagcgt aacgccagtt
     4861 ccggaaccaa aataaaattg cgactcgccc gccgctagag ctgacagcgc aacatttccc
     4921 gcccggacat agaccagccg cgtatccaga tcctgcttgc gaaaaaatcc ctgatcctga
     4981 gcgacgagaa gagcgcccgc cgtgcgttca ttgaaactgg cgtatgaaat caccgtctgc
     5041 gtagccgctt ggacgtatga agctacgaga agtagcgcaa ccgtaaaact tattcgaaga
     5101 cgcatgatca acctccacga tccttatact accggtgttc catggtttaa acgttcaact
     5161 tcaaaaagat gcaggtccaa tcgttccaac agttaagaca catcgatcgg gattgcgttt
     5221 tcttctgaat gtttggaacg attggaactg tcggaacgat taagttctca cttgcctctg
     5281 tcattgaacg tttgaacggg ttccggtgcc ccgacttcgg gcgagcaaga aggctcacat
     5341 acggggcgcg catgagcgca ggcgtacgta atcagtacgt cggagcgaat cggcgaggcg
     5401 caacgaagta gatgaggctt atcgctcgcc cgctcacgcc cagtacgtgg tgcggtctac
     5461 cggaatatgc tgcagaacct cacccggcac atactcttcg agccggattc tggcgagagc
     5521 ctcgtccgga catttggaca ccggcgagaa cggacggtcc agcggccgag tcgcgtcgac
     5581 gatcatcgcc gaagtcttgc gcgggctctc taatgatgga tcgatgagac tggcccgcat
     5641 gatttcgccg atgatcgaaa cttgtcggtg cggctgaacc cgtgtcgcca gcgcccagag
     5701 aatttccgtc gggttataaa catcgatatc ctcgtcgaag acgaagacat ttttacacca
     5761 gtcccatgtc aaaaccgcgg cggccgcgcg accgggatcc ccttcagaca tttttttcat
     5821 tgagataaac acatttaaca gcgcatggcg gccgctggta cacacggttt gaacgttggg
     5881 caccgcctcg cggcaccggc gcagatacgc gccttcgcgc gccagatcca tccacggctt
     5941 gtcgccctcg ggcgtaagaa tcgacatgtg catcgcatca cggcggcgcg tgatggcgcg
     6001 cacttcgtaa caagccgatt gctgaaagcc ctgcatgccc aaatagccgt tcacctcgcc
     6061 aaacggacct tcgaccattc gcttgcccgg cagcaaggcg ccttcgatca cgatctcggc
     6121 gtcggcgggg acgagtaggc gctcgcccca tgtttccgac ggcgtcaagc gcagcggttc
     6181 ttgcaggtac ccgccgatga tttcgtattc gctaatctcg aaggggccgc cgtaacaggc
     6241 acccatttga tagccgggat ggtggccgat cactgtggct accgggcatt cttcgttggc
     6301 ttcctcgtag ccgcggaaga tgcgccacag atgctgcaag gtcatgagga agcccgtatg
     6361 gtttcggtct ttgacttcca tgcgatgata cgaggcgttg tagacgccgc tgtggcgatc
     6421 ccgcgcaacg ctcgacatat cgatataagg tccgccatcc atctcgtgat ggcgcatcag
     6481 cggcaactga taaagatcga cttcgtcacc tttttgaatc acttctttaa tcggcgccgc
     6541 gcgccgatcg acgagaatag gcgcgatcct acctgcttcg cggcgcagga tctcgcgcgc
     6601 catttcggcg cgatccattt cagcgcgcac gcctagcgcc acctgcactt tcttctgggt
     6661 gttttcggtg ccgatggcaa gacggatccc gttcagctcc ccgtgtgggt ttaacggatt
     6721 gtcgaaaatc aaaataggaa aacgcttgag tgcgccaagc tgttttacga tcgcagtgac
     6781 atcgtaactg atcggattga ccgtcttcga aatcgtcacg acttcgttgg ggtaaatccg
     6841 gcgcatgtcg tcgagaaagg tgcgtagagt tttcggcatg tgatggttcg aggcacccgc
     6901 gggtcgggtg cctaaataag gctgcaacaa cagcctttcc gggcgacatc cgctctgagg
     6961 tttaccatgc ggctgatttc caaccttatg ttctcaggtc agaacgcgcc aaagtctctc
     7021 aatccctgga ccccttcttg gcaagcaggc caaccgacaa cctgcttcgt tcggaaaaac
     7081 gagcaatagt ccgagacccc aaccttttct attgttccat caccgaaggc gcgaacatct
     7141 cgtcgatctt caccaggcgc ggggtcagcc cctgctcaaa cgaatactgc gccgtggtct
     7201 ccagcacctt gcgattggcc gcgataccgt ggcggatgag cggcgtcgcc agcgccttgc
     7261 gggcctcggg cgggagtaaa cccgtttcaa ggtgataagc gaccgcctcc atgcgcgcct
     7321 tctcggcgat gtcgtttgcc cgcacgaatg ccttgaagag attgaggacc gtccatggat
     7381 gtctatccac gatctcgcgc ttgatcacca tgcaatggtt gatggggtag attcccgtct
     7441 tttgatagaa ccgtacgcct tcggcgtgag gatcggggaa gagagtcttg aaatcaggat
     7501 ggcgataaag atccaccgtc gaacggtcta ccagattatc cgtgccggcc aggtagcgca
     7561 gtgcgccgtc gagcctaccc tgcatcagca tttctcccat gttcgtgtcg ttcgga
//
LOCUS       DASUVS010000277        37001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1861735, whole genome shotgun sequence.
ACCESSION   DASUVS010000277 DASUVS010000000
VERSION     DASUVS010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 37001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37001
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861735"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..360)
                     /locus_tag="VF182_15190"
     CDS             complement(103..360)
                     /locus_tag="VF182_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438482.1"
                     /translation="MDFEKAKLNPAAVFKSPQEVVSSQELSRQQKIEILRRWEQDATL
                     MEVAEEEGMPGPQPKLLQPIRDALHALDYWPDTENSGPSKG"
     gene            677..1621
                     /locus_tag="VF182_15195"
     CDS             677..1621
                     /locus_tag="VF182_15195"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX6438483.1"
                     /translation="MARKPRVEFEGALYHVIVRGNHRRDIFRDESDRIAYLQRIEHYR
                     ERYRCIVYAYVLMSNHVHLLIETGAVKLSKIMQGIQFSYTQRYNRRYHAVGHLFQGRY
                     KAILCDRDAYLLELVRYIHLNPARMKTPQDPWKYRWSSHHAYLGENSPLKIQAQAVLE
                     QFGSKIRMARSGYLDFMKEGIGQGHLDRFYQTVEQRFLGDERFVDEIATHKKDRDPAT
                     IKVKFSRLVEGVASVYGIEAGRLLGTERKRTWVEPRSLLVYAAREWCGIKAKVLAEHL
                     HRDASMISRLHAAYVEKRDRRLELQLQHWLGVKSTTHA"
     gene            2493..2852
                     /locus_tag="VF182_15200"
     CDS             2493..2852
                     /locus_tag="VF182_15200"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6438484.1"
                     /translation="MNSKALKGNHALETAVEENRLSDMPTHSARGATETRKVERILAP
                     TDFSSSSQAGVRYALDAARESGAEVIVYHVITAKEIAAFGRVQREGALVDGRFYGLTE
                     TYKLHLRRFGRENPYRW"
     gene            2824..3102
                     /locus_tag="VF182_15205"
     CDS             2824..3102
                     /locus_tag="VF182_15205"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6438485.1"
                     /translation="MVEKILTDGNVGKGIRVREEVQFGTPGRNIVRAAAAEKADLIVM
                     STRRKGRWARLFSSDVTEHVSRYAHCPLITVPSEFSAAAQEDFYKKAA"
     gene            3242..3691
                     /gene="rnk"
                     /locus_tag="VF182_15210"
     CDS             3242..3691
                     /gene="rnk"
                     /locus_tag="VF182_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HEX6438486.1"
                     /translation="MAANLTNNEMAAKGKIYITEFDLKRLRGLIKHAKESWDKRAIQH
                     LDALEDELDRAEVIKPEDIPADVITMNSTFRLKDLQTGEEAAYTLVFPGTADSSNRRI
                     SVLAPIGIAVLGYRVGDTVEWEVPAGLKQFKVERVLYQPEAAFDYNS"
     gene            3935..4092
                     /locus_tag="VF182_15215"
                     /pseudo
     CDS             3935..4092
                     /locus_tag="VF182_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551634.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4557..5414)
                     /locus_tag="VF182_15220"
     CDS             complement(4557..5414)
                     /locus_tag="VF182_15220"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438487.1"
                     /translation="MAKDMRFFEEQGLDAQIVQVRSGQLAVSALAANEAQFYAASATG
                     ANIGAMAAGLDLAFIAGLVNKLDGDFVVSRKITQPHDLKGKLLGVQSIGGGVWTFAML
                     ALDHWGLIPERDKIQIRIVGDQAVLTQALISGTVDATYLGHAFSKVAQRSGFHVLADF
                     AKLEIPYQNIGIIARKSFLDRSPDIAERTLRALSNAVVYFQDPSNKSTVTAIMMKWLR
                     LPQREDAIAGYEAMRPLYSHRIAPTASGIRNTLRILSRADPKFNALKAEDLIDDRIIR
                     KLDKDGTFK"
     gene            6189..7262
                     /locus_tag="VF182_15225"
     CDS             6189..7262
                     /locus_tag="VF182_15225"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6438488.1"
                     /translation="MVRVPASFQIGPRFKEIRMIAMQFLFLFLGFTLYLANAKEASAG
                     QPAAVIEGAKKEGQLVFYGTMELTMSKKLAGMFEKKYPFIKTNIIRLGSERLATRVTT
                     EAQARSVQADVISESEMDFYGLFKKGFIEPYESRERDAFKNEYKDDKGSWTIGSETLA
                     VLAYNTNLLKPTEIPKDWIELTSPKWKGKVMIDENESKWMGGLFTVWGEERTLDFLKR
                     MAEQDVKVIGGHSQMHTLLAAGEAPIMVAALVHGLEQKKREGAPVEWIPLEPLISRQF
                     ALALTRGAPHPNAGKLYIDFLLSKEAQQEIASYGYTSGRKDVDSNVLKRLPSSMKIVP
                     VRPEMGERYNEYFKLYRKIMKLE"
     gene            complement(7451..8395)
                     /locus_tag="VF182_15230"
     CDS             complement(7451..8395)
                     /locus_tag="VF182_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438489.1"
                     /translation="MSKAENIDTEIEKKKESQGMTGPIDGPIIISEDEASGRNYTHRG
                     GGRLPGGRRYGFFDKTKGIGFSATMSVYSRERNNPRHKHDFDQVRFFPKGGENYGREV
                     LGPGDCIYIPENCSYGPTFTAEGCDENVRINLQFPGPSRREYLHSYDIMRATDELSKI
                     AKLENGYYRFPNGKNKDSYEAIVEHLTGKPVDYPPKRYSNYVVMHSNNHNWVQLEDRP
                     GVSVKHLGYFNDVGPNIKLIKLDPGAIVPMNQVGFQQVRFVLEGAIIYEGRKYDAVSS
                     MYFPANQTYPAIASESGAVLFIVQVAWTDKKPLPFCVI"
     gene            complement(8856..9458)
                     /locus_tag="VF182_15235"
     CDS             complement(8856..9458)
                     /locus_tag="VF182_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX6438490.1"
                     /translation="METPDTIREYWFGADLQDAAVTAHERARLWWSKNPEVDDEIRRR
                     FESCVIKAGSGELDHWELNPHDRLALILLTDQFPRSIYRDSAKAFAFDSKALSLAHTG
                     IDAGFDHSLRPLEKVFFYLPLEHSELLADQQRAVSLFQKLVDEAGADQKPTFAEYLDF
                     AVRHRDIISRFRRFPHRNEALGRISTPEELSFLQQPGSGF"
     gene            complement(9493..9792)
                     /locus_tag="VF182_15240"
     CDS             complement(9493..9792)
                     /locus_tag="VF182_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438491.1"
                     /translation="MAIPWFQIVKLVPEILSLSKQLLQQTRTDQSSGAKPLEDRVAEL
                     ELNERKQAELVASMAQQMAAMTDALTVLRKQVAAFRLISIISLLLAIGAIILALA"
     gene            10034..11110
                     /locus_tag="VF182_15245"
     CDS             10034..11110
                     /locus_tag="VF182_15245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX6438492.1"
                     /translation="MSIDFVTNQEIILAARRNLTQDIWDYLSGGAESETTMRRNRIAF
                     DSLALCPRVCVDVSKIDTSTTFLGQKLRIPVMMAPIGSLQTITPEGGVAVAKAAAEFG
                     TMNFVSSVTQPSLEEIAASTKHPKIFQLYIRGGLDWCEEIVERVKRAGYMALCLTVDT
                     AHYSRRERQMMNRWLPPNKRSETTRHFQATLTWEMMAAIKKMAGLPFILKGVATAEDA
                     KIALDHGVDVIYISNHGGRQLDHGRGTMDVLPEIVQVARGKADIILDGSILRGTDILK
                     ALALGCKAVTIGKLQGWGLGAGGQAGLVRVLELLEEELIIDMGLLGVTRIDQISEKYV
                     CKAPPVCFPNEMSAFAHLPGGQVR"
     gene            complement(11338..12126)
                     /locus_tag="VF182_15250"
     CDS             complement(11338..12126)
                     /locus_tag="VF182_15250"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438493.1"
                     /translation="MTKPQEPQAGERLSAQAAKAYVDYLYSTVSQASQERIHKGSFMA
                     QLRDGTLPLPVIRQFFKNWGHFSIELNGLDAVSYYTHLPFFVRNFELLGPFCAKVADK
                     LISPKAPGHVLLLLQTAEALGLSRKEMLEDPYLPAARAISDFCHKIFIDGSIAELWGL
                     HVFEESLSQWMREWYTALTAKYGFTNEQAIYFARREEADEESRNRGASDEKIVTGGFH
                     RNVLERVLQQEVEFRAGYSIEYCALTMIELHAQMNQAAMDNPYP"
     gene            12248..12604
                     /gene="queF"
                     /locus_tag="VF182_15255"
     CDS             12248..12604
                     /gene="queF"
                     /locus_tag="VF182_15255"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX6438494.1"
                     /translation="MPTVPSKRLETFPNPHPERDYEINMEAPEFTCVCPRTGQPDFAT
                     IRIRYIPDQLCVELKSLKLYLWSYRGEGAFHEDVINRILDDFVEACRPRKITVVGDFN
                     VRGGIHTTITAGYPSL"
     gene            12931..14307
                     /gene="dnaB"
                     /locus_tag="VF182_15260"
     CDS             12931..14307
                     /gene="dnaB"
                     /locus_tag="VF182_15260"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX6438495.1"
                     /translation="MAALEDNLRKVPPQNLEAESSVLGGILLENDAINVVLEVLRPED
                     FYRESHRKIFRAMIEILDRNEPVDLITLSDFLKGRSELEAVGGTSYLASLADFVPTAA
                     NISYYARIVREKAILRSLIGAATEIATRGYEEQGNVEEFLDTAEKVIFDISEKKIKAS
                     FVSVGDMIKDTLKTVEKLYERKEMVTGVPTGYEDLDKLTAGLQPADLIIVAGRPGMGK
                     TAFALNIATNAAYTGVGAAVFSLEMAKEQLVLRMLCSEAHVNSSKVRSGYLGERDFPQ
                     LAKAAGRLHEAPIYIDDTPAISVLELRAKARRLVRDRSKKIGLIVVDYLQLMRGMGTA
                     NNREQEISEISRSLKALAKELNVPVMALSQLNRRVEDRGDRRPMMSDLRESGAIEQDA
                     DVIMFIYRDEVYNKSDESKKGVAEVIVAKQRNGPIDTINLTFLSEFTRFENYTGRDDD
                     YYDEADEA"
     gene            complement(14339..14812)
                     /locus_tag="VF182_15265"
     CDS             complement(14339..14812)
                     /locus_tag="VF182_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX6438496.1"
                     /translation="MSKSSFRHPPENRYFEDYVPGAVHEFGSVLVDEEEVLEFGRRYV
                     PLSYHIDKEAAQKSIYGGLIASGWHTAALMMRLYTENYLSKIANLGSPGCDAIRWDKP
                     VFPGDRLSVRVTVIDARISRSKSDRGIIRSFVEVLNQNGEIAMSLEMVNFVRTRN"
     gene            complement(14823..15458)
                     /gene="thiE"
                     /locus_tag="VF182_15270"
     CDS             complement(14823..15458)
                     /gene="thiE"
                     /locus_tag="VF182_15270"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX6438497.1"
                     /translation="MTSRKSARVTLPALYAILDPEQIGTSSADAVLRQLLFGGAKIIQ
                     LRAKAMSSRDFLQLAQLARELTTPLNCKLIVNDRIDIALGSAADGVHLGQEDLPLEVG
                     RKLLGERIVGVSTHSLEQAKEAEANGADYIGFGPIFGTATKNTGYTARGLEMLARIRA
                     AVALPIVAIGGITEANIQSVWRAGADSGAIISDILKADEVAVKVSAILARR"
     gene            complement(15460..16905)
                     /locus_tag="VF182_15275"
     CDS             complement(15460..16905)
                     /locus_tag="VF182_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6438498.1"
                     /translation="MDQGKILVAEDEESLRFVLKKALEEEGYWVHTAVDGSGARQSLS
                     DCNFDVALLDIKLPDVDGLTVLKESKDDGIDTAMIIMTAQNTMRNAIGAMKSGAFDYI
                     TKPFDLDEVLVLVKRALDSRKLTRDFRELKEEVKKRFEPGVNIIGTSSSMQRVYKIIG
                     QVVDTQATILIQGESGTGKELVAKTIHYNSPRWNQPFIAINCAAIPRDLLESELFGHE
                     RGAFTGALDRRIGKFELAQGGTLFLDEVGDIPLELQTKLLRVLQDREYSRVGGREMLK
                     ADVRILAATNQDLDKAVHDKRFREDLYFRLRVIPIYLPALRERRGDIPLLIGYFIDKI
                     NREMGIQISGVSPDALRLLEEHPWPGNVRELENTLIRAAVLASGPILFPKDFNLQNKQ
                     SEAAPEIDQLSLEEVFRHKLEDYFRRTEGVEVDNLYPLVIERIERPLIELTLKKTRGN
                     QIRAAQILGINRNTLRKKITDLRIELKKESS"
     gene            complement(16907..18016)
                     /locus_tag="VF182_15280"
     CDS             complement(16907..18016)
                     /locus_tag="VF182_15280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6438499.1"
                     /translation="MKPIESPSAAVERKISWEQILSSVEDGVITLDRSGKVSFFNEAA
                     EILTDLAASRALSLSFLRIFRKEPWLLELVKKSFPPLQESARGEGDLVSRWGKRVPVG
                     VTVSPLVETDGKCLGSILLVRNLTQHKEFEEDLKRADRLAMVGTLAAGLAHEIRNPLG
                     GIKGAAQLLRRSLDPGSSLIEYTNIMNREVDRVNLLIEQLLDLSRPAKLEIVPLNIHQ
                     VLEDVLLLELKTQDAPTVNIRKLFDPSLPPIRGDRNRLTQVFLNLVKNGYQAMNGSGT
                     LTITTRLETDRHVRGQGAERSRFIWVEIADQGSGIKEEDLPYIFSPFFTTKNSGTGLG
                     LAVCYWIIREHGGIIRVESFAGKGSTFKVSLVVAD"
     gene            18182..18655
                     /gene="rimP"
                     /locus_tag="VF182_15285"
     CDS             18182..18655
                     /gene="rimP"
                     /locus_tag="VF182_15285"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX6438500.1"
                     /translation="MELDRTVSRIWEIATQVADGQGMEVVDVELRSEGSRGGRVLRLY
                     LDKEGGPNIDELGRVSRELSELLDAQDIVEGSYTLEVSSPGINRPLKKPEHFRRFTGK
                     RVRIRTRDMIEGRRSFLGTLSEVTGEKIKVEVEGKQYEIPFTTIEKSNYEHDWSA"
     gene            18656..20023
                     /gene="nusA"
                     /locus_tag="VF182_15290"
     CDS             18656..20023
                     /gene="nusA"
                     /locus_tag="VF182_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX6438501.1"
                     /translation="MQQDLNRVIEQVSKEKGIDKSILIAALETAMVSAAKKTFGHQRK
                     IEAQFNPEIGEVELFEAKTVVENVTDAAVEVSLNEAKESLDPDAEIGDELLSKLDSSM
                     FGRIAAQAAKQNIVQRVRDAEREIIYNEFKGREGQLVNGIVQRFERRNIIVNLGRTDA
                     ILPEKEQVPRERYRQGDRIRAYIVSVEITSRGPQIVLSRTHPGMLIQLFAQEVPEIYE
                     GIVEVKGAAREPGGRAKIAVISNDSDVDPVGACVGMKGTRVQAVVQELRGEKIDIVHW
                     IPDQAEYVCRALAPAKVSKIIIDDDEHSMEVIVPDDQLSLAIGKKGQNVRLAARLTGW
                     RIDVRSEAEAEEETRRARMSIGAIPGINDFAAELLYQAGFKSAEEVSESELEDILDVE
                     GISKEKAESLHKSAKEYVAEKRLKAEAEKANAAEHTHETAPSETASPTETTAAPEAAE
                     GITRG"
     gene            20312..23095
                     /gene="infB"
                     /locus_tag="VF182_15295"
     CDS             20312..23095
                     /gene="infB"
                     /locus_tag="VF182_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX6438502.1"
                     /translation="MGKMRVHLLAKELEIETKELIAQLERLGIRGRKAQSSLEDEEVT
                     RVRAALAMQEKPHVHVGQEKVVADRVVTADDQSSGEIQARETIVERRVRANVIRRRTS
                     RVEVPTETHEAETSPAEAVATPLPPSAPNFEDLAATMPSYEDDSSLTAIAPESDTSHH
                     EEAEETPGEIDEAAPMEEPQAQAVTAPDRQPVPTAPAPPPAAPPAVAPAATPKEPLRG
                     ARVLGRIDLKRTVTIAPAAPAAPRRPVPGSPGVPPAANDATRPARDGEAAKPATGEAE
                     KPKAGRHKKRVVKKQDVLEFREKELRSGRIPKKKRALPGKEQRKTEITVPRASKRVIR
                     ISEVITVGDLAREMGIKAGDVIKKLMSLGMMASINQVLDVDTATLVAADFDYQVENVA
                     FDAESMLEVEQVEEDSMLVSRPPVVTIMGHVDHGKTSLLDAIRSTHVTAQEHGGITQH
                     IGAYHVKVDGRSVTFLDTPGHEAFTAMRARGAKVTDIVVLVVAADDGVMPQTVEAINH
                     ARAAEVPIIVAVNKIDKPDADLERVKRGLSEHGLVSEDWGGDTVFAPVSAKTQEGIPH
                     LLEMLLLQADILELRANPDKLARGTIVEAKLDRGRGPVATVLVQEGTLRVGDSFVCGV
                     FHGKVRAMINDLGQKVELAPPSSPVEILGLQGVPQAGDSFIAVADESKARQVAEHRHT
                     KQRETELVKSSKVSLEELYDQIKTGDVKELRVVVKADVQGSVEALTDALTRMSTTEVK
                     LRVLHGSVGGITESDILLAAASNAIVIGFNVRPESKGQALAAQEGVDVRLYTVIYEAV
                     ADVRAAMEGMLEPTFREQTQGRVEIRQIFNIQGVGTIAGCYVTEGKIQRGNLVRILRD
                     HVVVHEGKLASLKRFKDDVREVSAGYECGLSIEGFHDIKQGDVVEAYERTAVVRRISP
                     VASGREASHYAGR"
     gene            23149..23421
                     /locus_tag="VF182_15300"
     CDS             23149..23421
                     /locus_tag="VF182_15300"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX6438503.1"
                     /translation="MKVNLLLPENHSLKGKRGVLKRIQARVANRFNVSVTECGDQDLW
                     QSAVLGFGLIGTHKEIIEKTLNKIVDFIDEIGLAQVVDCDIEFFYC"
     gene            23434..23778
                     /gene="rbfA"
                     /locus_tag="VF182_15305"
     CDS             23434..23778
                     /gene="rbfA"
                     /locus_tag="VF182_15305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX6438504.1"
                     /translation="MDYQRSERVGELLTEVIAEVLREDIRDPRITAVTITAAKVTKDL
                     RQARIYFTILGGTAENKHSVLAGLKSALGFIRSRIGKQLKLRFVPAIEFFYDESVDEA
                     ERIENLLRQVKQ"
     gene            23839..24738
                     /gene="truB"
                     /locus_tag="VF182_15310"
     CDS             23839..24738
                     /gene="truB"
                     /locus_tag="VF182_15310"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX6438505.1"
                     /translation="MTKSGLLLVDKPEGPSSAHIVHRAKVALGAKKVGHLGTLDPFAS
                     GLLLLGINEGTKISDIFLRAPKSYCGVLTLGVATDSQDSTGVVIKTLPVPSLADEDLR
                     LLEQRFRGELQQTPPMFSALKRDGVRLYKLARQGKEVPRQPRTIHIDSIRLRQINRTE
                     LSIDVTCSRGTYVRTLAADLGEALGCGAHLKSLRRIACGHLRIDQAVTLDELEQFRTR
                     AQSAMISLSDALGHLRGISWDQRWLARLRLGQQEALGQIGKPLEKQELVRLLDARGDL
                     VAVIGWSDDIPGDGWRLLRVFKE"
     gene            25032..26807
                     /locus_tag="VF182_15315"
     CDS             25032..26807
                     /locus_tag="VF182_15315"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX6438506.1"
                     /translation="MSTSAVKAPANPHIQKWVKEMAAMCQPDNVYWCDGSESEREELT
                     RVAVETGDLMPLNQDKLPGSYLHRSALNDVARTENLTFVCTPTEEEAGPNNNWMAPAE
                     SYDRLAKIFTGAMRGRTMYVIPFLMGPASSAFSKVGVQLTDSVYVVLNMRIMTRMGKV
                     ALDHLGGSNDFTRCLHSKADLDMDRRFICHYPQDNTIWSVGSGYGGNALLAKKCLALR
                     IASKLGQKEGWLAEHMLIVGIESPDGEIHYVCGAFPSACGKTNLAMLVPPASMKGWKI
                     HTIGDDIAWLRVGSDGRLWAINPEAGFFGVAPGTGSKTNPNAMQTVQRNTIFTNVALR
                     SDGTVWWEGHDDPVPANAIDWRGKAWDPKSGHPAAHPNSRFTVSLKQCPTYSPEWEKP
                     QGVPISAILFGARRNKVVPLVYQSFDWQHGTFLGATLASETTAAATGAVGVVRRDPMA
                     MLPFCGYNMGDYWGHWLSIAKRATNPPKIFRVNWFQRNDQGKFIWPGFGENLRVLRWV
                     IERAKGGGQADETAIGYVPKTSSLNGDGLGVSKSDLEQLVTVDRDAWKANLKSQGEYF
                     DKFGDHLPAGIKEEHRALAERLKGG"
     gene            26820..27131
                     /locus_tag="VF182_15320"
     CDS             26820..27131
                     /locus_tag="VF182_15320"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438507.1"
                     /translation="MERGHHDIGGLPGAGPIDKSQHELSDWEILADAINQALGAKGVK
                     RTDEMRRAREEMDSEAYREMNYYERWIASMEAILIEKGILKKDEIDKKVVEFEKKWGE
                     P"
     gene            27128..27454
                     /locus_tag="VF182_15325"
     CDS             27128..27454
                     /locus_tag="VF182_15325"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEX6438508.1"
                     /translation="MIHPATGRFRPGDAVRVLNEHRPGHIRTPFYIRGKTGWIERVYG
                     DFLNPESLAFGGDGLPRRTLYLVAFKQGDVWGAAQAPTDKLFVDIYDHWLEPASYGGQ
                     RDEHIR"
     gene            27438..28067
                     /gene="nthA"
                     /locus_tag="VF182_15330"
     CDS             27438..28067
                     /gene="nthA"
                     /locus_tag="VF182_15330"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017996702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEX6438509.1"
                     /translation="MSTSGDDHDHEHPRNELSAQAKRIYAIRDLLLEKGVISEKDIQD
                     QIEYQEARSPANGAKLVARAWVDPAFKARLIADPKAACAELGIDATALNEFVVLENTK
                     KVRHMVVCTLCSCYPRPILGRPPDWYKSFNYRQRSVNDPRGVMREFGLVLPDDVEVRV
                     HDSTADMRYLVLPLRPKGTEGLSEVELARLVTRDSMIGVMDALPKLPET"
     gene            28089..28265
                     /locus_tag="VF182_15335"
     CDS             28089..28265
                     /locus_tag="VF182_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438510.1"
                     /translation="MLTYAQAPRAIGQDLQNHGALAYEINVNQAEYVFNVIATYRRRT
                     ISCLFVIRGKFSSK"
     gene            28689..29189
                     /locus_tag="VF182_15340"
     CDS             28689..29189
                     /locus_tag="VF182_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438511.1"
                     /translation="MRRLARLHSVNYSTALRCIGQDLESRGLRTLDIVADNAGYIVLA
                     GYQPPPAPMPVSLQYSPADILELDRTGQEQRGTSSAAKEFVTLVQILRAIGGYLDKNQ
                     ARLIRISNSEAPGSTPAFSVEYETKEGERVVDDRDGTAIYDMCVSMYKQRRKVDHSAK
                     LPSHGR"
     gene            29251..29457
                     /locus_tag="VF182_15345"
                     /pseudo
     CDS             29251..29457
                     /locus_tag="VF182_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006655246.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
     gene            complement(29793..30554)
                     /locus_tag="VF182_15350"
     CDS             complement(29793..30554)
                     /locus_tag="VF182_15350"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6438512.1"
                     /translation="MRLQSKVVLITGAAHGIGKAYARRFAEEGAEVVIADIDSQGGKT
                     VAQALTDSGFAAWARATDVRNYLDVEALIRETADKFGRIDVLLNNAAIYVTEKLWKGP
                     VEELELAEWDRVIEVNLKGVFLCCKAAIPIMKRQRSGKIINIASGTFFSGSGNMPHYT
                     TAKGGVVALTRVMARQLGEWNINVNCMTPGSTMSEDSVTDEVIERRQGSTGKRAFPRI
                     ETPADIIGTAVFLASSDSDFITGQLLVVEGGGIMH"
     gene            complement(30606..31463)
                     /gene="hemQ"
                     /locus_tag="VF182_15355"
     CDS             complement(30606..31463)
                     /gene="hemQ"
                     /locus_tag="VF182_15355"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HEX6438513.1"
                     /translation="MKQPLNKLAGDLPATPLTLDGSYLLHQMFRVRWAAWRALSAAEQ
                     EAALDHAIATFHDMEDNKQEATGLFAMLGHKGDLMLVHFRRTLDALNRAEFELAQLKL
                     LDFLEPMTSYLSVVELGLYEASVKLYTDLMARGLRPGTAEWNREVEAELAKQRQAAAP
                     RLWPEIPQRRYLCFYPMNKFRGESRNWYNEPIQERQRMMREHGMIGRHYAGRVNQIIS
                     GSIGFDDWEWGVDLFADDPLVFKKLVYEMRFDEASAIYGLFGTFYVGLRFPADQLKNL
                     FQGKAPGFS"
     gene            complement(31521..32147)
                     /locus_tag="VF182_15360"
     CDS             complement(31521..32147)
                     /locus_tag="VF182_15360"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX6438514.1"
                     /translation="MGSIEALSLAVGTAWTSGINLYATVAALGLAGRFEMIQLPQSLD
                     VLTNPIVIAVACVMYAIEFFADKIPYVDNGWDILHTFIRVPAGAILAARSLGDMSPGL
                     EFAALLAGGSIALAAHGTKATSRLAINSSPEPFSNWIASFVEDVVVLGGIWIIFNHPW
                     LMLALVFVFLALTVWLVPKIFRVAKRGFRALRTRLGVIKPEQASTSAP"
     gene            complement(32226..33659)
                     /locus_tag="VF182_15365"
     CDS             complement(32226..33659)
                     /locus_tag="VF182_15365"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6438515.1"
                     /translation="MTDVTDMRGYVELLEEMGELRRLEGVDLDLEVGALTERAAEKEG
                     AALLFSNFKDYPAGFRVISNVFRTCRRTGPAMGIDPELKGVDYLNAWRKKLAAYKPVP
                     VQQVEGGPVFENQMIEDQVDLYKFPVPKWHDLDGGPYIGTGCGVITKDLETGKINIGT
                     YRVMIQNKNTLSVKMNMGKHGRLAFERARDAGKPLPIAITFCQGPAIFLAAQMPLPPD
                     VNEFEFAGYLQGAPVPVARGAVTGLPIPANAEIVVEGELPPMRDQDMPKEGPFGEWPG
                     YFTEENVGETPIMNVKRIYYRNDPIILGAPPLKPPASYLPIPLGAATLWEQLEKAGVP
                     EIKGVWGFVYGGQPGPFTIISIKQRYAGHSKQALLVAAGARAGAYGGKFVVVVDDDID
                     IANPADVIWAIATRCHVREGLDVVKGVWASVCEPALPPEERSPRGYTSDRVLIDACRP
                     FKWMDQFPPVNAFTSEFKDRIESKFKI"
     gene            33920..35242
                     /locus_tag="VF182_15370"
     CDS             33920..35242
                     /locus_tag="VF182_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX6438516.1"
                     /translation="MRRLFSNLTLAFFLLAASAQDSFAAVQEFLLENGLKILLLEDHK
                     SPAVTFQVWYRVGSRNERDGKSGLAHFLEHMMFKGTPRTGPEEYSQIIAKNGGQTNAF
                     TTADVTVYFATMSRDKIHIQLELEADRMVNALLGEEYFEAEKKVIQEERRLRVDDNPA
                     AALGELAGAITYMVHPYRRPVIGWMHDIENLTRQDLVDFYKLYYSPNNAFIVVTGDFT
                     TAEILPKIKAAYGNIPRGAEPPKVHTLEPPQLGERRAILKKEAELPIVTANYHAPNLK
                     SPDNYALDLLTVVLAGGRSSRLHQDLVYHKRLVRGVDADYSGASIDPTVLSVTAQLLP
                     GKDPAPIEREIDGLIDKVKAELISDRELEKAKNQVEAAFIFSRDSIFGQAMKIGFYEV
                     AGDWRLMDRYLEGIRKVNREDIRRVARQYLDRDRRTVGTLIPVKPKTP"
     gene            35239..36561
                     /locus_tag="VF182_15375"
     CDS             35239..36561
                     /locus_tag="VF182_15375"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX6438517.1"
                     /translation="MKKMFFLVLVVGSLLVATGFGRTAVTPQRSVLNNGMVLLTSEQR
                     TLPMVSIEMLIHAGARYDAPKQDGLANLTAELITEGTKRHAALQISESLDFLGATLSA
                     RGGEDLASVSMTVLKKDLPAGLELLAEVLTSAVFPQEEIERQKQAVVASIRAKQEQPG
                     EIAEMRFAGAIFPQTRYGRPVEGTETSVKAIPQASLREFYQRYYRPNRSIMAIVGDIS
                     HEEMIEALTQAFRSWNKGEPPAKPLVLGAAPETGTIRVNKDLTQANLVLGQRGVGREN
                     PDYYALQVMNYILGGGGFSSRAMDSIRNQRGLAYSVYSYFDADKGRGTFQFVMQTKNE
                     TALQAIAIAKEEIRRIREAPVSEAELNDARDYLTGSFPLRFDTMSKVANFLAQAEHFE
                     LGLDYIQRYPDLIGRITIDDVKRVAQKYLDPEKMVTVIVGNQTIIADK"
     gene            36683..>37001
                     /locus_tag="VF182_15380"
     CDS             36683..>37001
                     /locus_tag="VF182_15380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6438518.1"
                     /translation="MQKVLIIEDHADMRELLSWQLELMGFMPILAKHGKEGIEKALAE
                     KPELILMDIMMPGMDGWETCTQIKCDAVTADIPVILLTGANDRDLTQHANAVGASALL
                     QKPC"
BASE COUNT         8689 a         9701 c        10282 g         8329 t
ORIGIN      
        1 cgatttaact cacgctcctg gatgagacag ggcgggggag cattgagggg tcaggttgaa
       61 cggagttgat ttgcgcatcg cgactttctt gcggtccgct tctcagcctt tgctagggcc
      121 cgagttctca gtgtccggcc aataatcgag ggcgtgcaga gcgtcgcgga tcggctgcag
      181 cagtttgggt tgcgggccgg gcatgccttc ttcctccgcg acttccatta acgtcgcgtc
      241 ctgttcccat cgccgaagga tttcaatttt ttgctggcgc gacagttctt gacttgaaac
      301 cacttcttga gggctcttaa aaacggctgc gggattcagc tttgcctttt cgaaatccat
      361 tttgcttctt ctttcggtct atgaaatcgc tgatagcaaa tagaaagtaa gtcttggcta
      421 acgcttagtc gcctatctgg tcacagcatc ctgtaggaga actgattatg ccaaccaagt
      481 tttagcctct ttagtcctct gaatcaatgc cgctcaagtt gtttccacgg tccgaaggct
      541 acgaaacagg cggccgcttt tccacggcgt gccccacagc agccagcggt cagcccaatc
      601 catccgtgcc aagggggcgg gttggtgcca ggcatgagtt attgacttat tggggtgctt
      661 agcgatagaa gcagaaatgg cccgtaagcc gagagtcgaa tttgaaggcg ctttatatca
      721 cgtcatcgtt cggggtaatc atcgacgcga tattttccgg gacgaaagcg accggatcgc
      781 ctacctccaa cgcatcgagc attatcgaga gcgctatcgc tgcatagttt acgcctacgt
      841 gctgatgtcc aatcacgttc atctactgat cgaaaccggc gcggtgaagc tttccaagat
      901 catgcaaggg attcaattca gctatacgca gcgttacaac cgccgttatc atgcggtggg
      961 gcatctattt caggggcgtt acaaagcgat cctgtgcgat cgcgatgcgt atttgttgga
     1021 gctagttcga tatatccatt tgaatccggc acggatgaaa acgccacaag atccttggaa
     1081 atatcgctgg agtagccatc acgcatacct gggagaaaac agtccgctca aaattcaggc
     1141 ccaggctgtg ctggaacagt ttggttctaa gatccgcatg gcgcgaagcg gatatctcga
     1201 ttttatgaaa gagggcattg gccaggggca cctggatcgg ttttaccaga cggtagagca
     1261 acgattttta ggcgacgaga ggtttgtcga cgaaattgcg acgcacaaaa aggacaggga
     1321 tccggcaacg atcaaggtta agttttcccg attggtagag ggggtggcgt cggtgtatgg
     1381 catcgaagcg gggcgactgc tgggcacgga acggaagcga acttgggtag agccgcggtc
     1441 gcttttggtc tatgccgcgc gggagtggtg cgggatcaaa gcgaaagtct tggcagaaca
     1501 cctgcatcgc gacgcatcga tgattagccg gttgcatgcg gcatatgtgg agaaacggga
     1561 taggcgatta gagttgcagt tgcagcattg gctgggcgtt aagtcaacaa ctcatgcctg
     1621 accccgctga cttatgcctg accccgctga cttatgcctg accccgctga cttagaagtc
     1681 aatttttggt ggtgaaaaga gcctgtaaga atgccgcggg aaccgacttt ctgcctggtc
     1741 acacttggca catcacgcag caatgcagag aaggcagttc caatcgttcc aaatgttcca
     1801 attgtccaac cgcttcgctc cgttcaagac tttgcttaag atggggatct gacctaactc
     1861 ggcggctgcg atcggcgagt cccatctgcg ctaggttttc gaagcgaaga agcttcggtc
     1921 tttcggtact caattagatc gtaacctcca atcacgttca ccttttgatt agagacactg
     1981 atcaaaggct atcaccgata gcatgcagct gatcgctgac cgcagcggct aagtatagaa
     2041 tgagcgcaaa gacgccacgg cgtcggcgac ctgatacgtc tttgggatct taactctact
     2101 caggcatcca gcgatcaagg catcagcagg gaatcgaagt gcggtgaatg acccttgaat
     2161 ttacagcttt cgtggttaac ttacatatgt gccgcggcac cgaagtgtcg cgtaaaaatg
     2221 caagcacaag gggtcatcct gaaatctgat ctgcttcgca cgtacgcgca gtccaatcac
     2281 aaccaaacgc cggcgcaatt ttacaccctt taggcaaaga ccaacgcgcc acgcggcgcc
     2341 attaatttaa ttgcggttac ccgttttggg ctctacgtcg ctcgtccaac ggattggacg
     2401 tgggcggata gaattgccgg cgtgtgagtt gctgatagtg attaatttaa aaaacaagag
     2461 gaagagagta cttgatcgtg ttggaaagtg aaatgaatag caaagctttg aagggcaatc
     2521 atgctcttga gacggcggtc gaagagaacc gtctatcgga tatgccgact cacagtgccc
     2581 gtggcgcgac ggagacgcgc aaggttgagc gtattttagc ccctactgat ttttcgagtt
     2641 cctcgcaggc tggagtccga tacgcgctcg atgcggcgcg ggagtcgggc gccgaggtga
     2701 tcgtctatca cgtgatcacg gccaaagaaa ttgcggcgtt cgggcgcgtc cagcgagaag
     2761 gggctcttgt agatggccgg ttttatggac tcaccgaaac ctacaaatta cacctgcgcc
     2821 gatttggtcg agaaaatcct taccgatggt aatgttggta agggaataag ggtcagggaa
     2881 gaggtccagt tcggcactcc gggaagaaac atcgttcggg cggccgccgc ggaaaaggcg
     2941 gatctgatcg tgatgagtac gcgccgaaag ggcagatggg cgcgattgtt ttcgagcgat
     3001 gtgacggagc atgtgagccg gtacgcgcac tgcccgctca tcaccgttcc gtcggagttt
     3061 agcgccgccg cgcaggagga tttttacaag aaggcggcat aagaatcgct cgatttttgc
     3121 gctagaatga gggtgatcgg cgcacgcggt atgtttccgc ctgcgctcga ttgcactcgc
     3181 gcctttcagc gcagctcgta tgttccggct caaagaggcg cgcacgacga aaggagatcg
     3241 tatggcagct aatcttacga acaacgaaat ggcagcgaaa ggcaagatct acattaccga
     3301 gttcgacctc aagcgtctca gaggtttgat caagcacgcg aaggaatcgt gggacaagcg
     3361 agcgatccag catctcgatg cgttggagga tgagcttgat cgcgccgaag tcatcaaacc
     3421 cgaagatatt ccggctgacg tgattacgat gaattcgacc tttcgcctga aagatttgca
     3481 gaccggcgaa gaggccgcct atacgctggt gtttcccggc accgccgact cctccaaccg
     3541 caggatctcg gttctggcgc ctatcggcat cgccgttttg ggttatcgcg tcggcgatac
     3601 tgtcgagtgg gaggtcccgg cggggctgaa gcagttcaaa gtagagaggg tgctttatca
     3661 gccggaagca gcttttgatt acaactcata atcctgactc gtcttgattc cccgtcgcgg
     3721 ttcggcaacg catgttggcg cggccctttg ccgggatgtc ctccccatgt aggtaagagt
     3781 ccaggcggtc ggagcgaagt accgcgtgcc tcgacgctgc aagttcgcat aagaggcact
     3841 gtttaagacc ttagaagcga atttttcgtg gtgaaaagag cctttgagaa tgccgcgatc
     3901 ggaaccgcca ctttctgcct cgtcacattt cggcatgtca tgcggctgct gccatcaaaa
     3961 acaatttctc ttgaagttcg cgcgtgacag acgcgcgctt tccgtactgg ctcttggaag
     4021 caaacggttt ggactgtgtg tgtgctcgac tacgccgtga catccaatca cgtccacctc
     4081 ctgttgaagg ataccgccga gatgtgatgc tccatagcat gcaactcatc cgccggatga
     4141 tcgggacaga attacagacc gggagggacg gcacggagcc gttcaaccag agattagttc
     4201 agtatcggtt attcttggcg aaacgtaact ataagatgcg cggaagctga acgggcggct
     4261 catcgtcatt ccaggcactc ggctctaaaa taccgtcatg tcagcgataa gaatttgctt
     4321 tggtgctcaa tatatcaact aaggtcatcg cgacgtagat cttgttcggc caatgttgat
     4381 gacttagaga tgaagtgacg aaaaatgtga gcatagagct agtactgcgg tcatgtgccc
     4441 tcagggcgat cattgagtat gtgtagcgct tagcgaataa tgcgaatcac aatactaaat
     4501 cggcggatgt cgaatttaac gtctgaaaaa cacgaactct gacatttgat ttgtagtcat
     4561 ttaaaagttc cgtccttatc aagctttcgg ataatcctat cgtcgattag gtcttcggct
     4621 tttagtgcat tgaacttggg atcggcgcga ctgagtatgc gcagggtatt gcgtatccca
     4681 ctcgcggtgg gagcgatgcg gtgactgtag agcggccgca tcgcttcata accagctatc
     4741 gcatcctcgc gttggggtaa acgcagccat ttcatcatga tggcggtgac agtagattta
     4801 ttcgaagggt cctgaaaata gacaactgcg ttgctgagtg ctctgagagt tctctcggcg
     4861 atgtcgggcg accgatcgag aaagcttttg cgcgctatga tacctatgtt ttgatatgga
     4921 atttccagct tggcgaagtc cgctagaacg tgaaaaccag agcgttgcgc gactttgcta
     4981 aaggcatggc cgagataagt tgcatcaacg gtgccgctaa ttagagcttg agtcagaact
     5041 gcttggtcgc cgacgattct gatctggatc ttatctcgct cgggtataag tccccagtga
     5101 tcgagggcta gcatcgcaaa ggtccatacg cccccaccga tgctctgcac acccaagagc
     5161 tttcccttga gatcgtgtgg ctgtgtgatc tttctcgaaa cgacgaaatc gccatccaac
     5221 ttattaacga gtcctgcaat gaaggctaaa tccaaaccag cggccatcgc tccgatgttt
     5281 gcgccggttg ccgatgcagc gtaaaactgc gcttcattgg cggccagagc agacacggcg
     5341 agctgcccgc tgcgaacttg aacgatttgc gcgtctaatc cctgctcctc gaaaaatcgc
     5401 atatctttgg ccacgaagat agcactccgg tgctcgttga agccggcgat tacaagaacg
     5461 attttctgga gtggctgggc ggcatgcgca atcatggaac tgcagactaa gcataagaac
     5521 aggagagcgc cgagcgtaaa ttgaggtaga ttgcgaactg caagaatcat cgcgaagcaa
     5581 aaagaagctg gtttagctat atttttgaaa tcttatctgc cgtaaatttc ccgataggat
     5641 ccttctgtct ccaatgcttg caagaatcgc aaattaacaa actcttcaag tcttggctag
     5701 gtgtttcccg atgtgattgc ccatagcatc cactcatcgt cgtttcaggg cttagaggca
     5761 gcttttcgat gccgaaaagg gtttctaatg aagacgatgc cacgcgccaa ccgctatttc
     5821 ctgcctggat acgtctggca catcacacat caacgttgaa ggccggttca aaatgttcca
     5881 gccgttcaac cgcttcgctc cgttcaagac gtttcggatt acgaccgcca atatcgaacg
     5941 gtttcgcgga tcccaagatt ttcaaactgc tcaccgccaa taacgactta gtcggggcag
     6001 accactctcg aaccgcgact cattgcagct ctgggcaaat ctcttgatga agtcgcgtct
     6061 aatccggtgg acttccgcca gattcgcgaa gaatatccat tacgcattgc tcggcgtcag
     6121 gtgcccgcaa aaatctgcgg cgttagcctg ccccagcggt tgactaccta ggaatttgga
     6181 atattaaaat ggttcgggtt cccgcctcat tccaaattgg cccacgtttc aaggagatta
     6241 ggatgatagc aatgcagttt ctatttttgt ttttgggttt cactctttat ctcgcaaacg
     6301 ctaaggaagc gtccgcgggc cagccggccg cagtgattga aggagccaaa aaggaagggc
     6361 agcttgtttt ttatgggaca atggagctca ccatgtccaa aaaactggct ggcatgtttg
     6421 agaagaagta tccgttcata aaaaccaaca tcatccgcct cggctcggag cgtctcgcca
     6481 ctcgcgtcac gacagaagct caggcaagaa gtgttcaggc cgatgttatt tcggaatcgg
     6541 aaatggactt ctatggttta tttaaaaagg gctttatcga gccgtatgaa tcgcgtgagc
     6601 gcgatgcctt caaaaacgag tacaaggacg acaaaggttc ttggacgatc gggagcgaaa
     6661 cgctagcggt actagcgtac aacactaatc tcctgaagcc gacggagata ccgaaagatt
     6721 ggatcgagct gacttctccc aaatggaaag ggaaagtcat gatcgacgaa aacgagagca
     6781 aatggatggg tggtcttttt actgtttggg gtgaggagcg gacattagac ttcctgaaga
     6841 gaatggcgga acaggatgtt aaagtgatcg gcggccattc tcagatgcac actcttcttg
     6901 cagcgggcga ggctccgatc atggtcgcag cgttggtaca cgggctcgaa caaaaaaaac
     6961 gtgaaggagc gccggtggaa tggattccgc tcgagccgct cattagtcga cagttcgccc
     7021 tcgcattgac gagaggagca ccacatccca acgcgggaaa actctatatt gatttcttac
     7081 tgtccaaaga ggcccagcaa gagattgcga gctacggcta tacgagtggg cgaaaggatg
     7141 ttgattctaa tgtcctcaag cggctgccga gttctatgaa aatcgttccg gtacgccccg
     7201 aaatggggga acgctacaat gagtacttta agctttatag aaaaataatg aagcttgaat
     7261 aggccggacg ctatgacgct atattgtgta ttacaggaac gaaagcgttc ggccagaagc
     7321 tcgtagcggt tcaagcacgt ggtagtaaag ggtatgacgt ggcggaggtc gcgttgcaca
     7381 ggagagagtt cctcgattaa acggcctgtc gcctcgtgct gaaagcacag ggcgacagac
     7441 atcgctaaat tcagatcacg caaaacggaa gcggcttctt atcggtccat gcaacttgaa
     7501 cgataaagag taccgcgccg ctctcactgg caatggcagg ataagtttga ttggctggga
     7561 aatacatgct ggaaacggca tcatatttcc tgccctcgta aatgatcgca ccctcgagca
     7621 caaacctgac ttgctggaat cccacttggt tcatcggcac gatcgctccc gggtcgagct
     7681 ttatcagctt tatgttaggc cccacgtcgt tgaaatagcc aaggtgtttg actgaaacgc
     7741 cgggccggtc ttccaactgt acccagttat gattgtttga atgcatcaca acatagttgc
     7801 tatagcgctt tggcggatag tcgacgggtt tgccggttag gtgctcgacg atcgcttcgt
     7861 agctgtcttt gtttttcccg ttcgggaatc gataatagcc gttctcaagt ttggcgatct
     7921 ttgagagttc atccgttgct ctcattatat cgtaggaatg gagatattcc cgccgcgacg
     7981 ggccgggaaa ttggagattg attctaacgt tctcgtcgca gccttctgcc gtgaaagtcg
     8041 gtccgtacga acaattttcg ggaatataaa tgcagtcacc cggtccgaga acttcacggc
     8101 cgtaattctc accgccttta ggaaaaaatc tcacctggtc aaagtcatgt ttgtggcgag
     8161 gattattacg ttcgcggctg tataccgaca tggtggcgct gaaaccgatt cctttggtct
     8221 tgtcaaaaaa gccatagcgg cggccgcccg gcagtcttcc tccaccgcga tgcgtgtagt
     8281 ttctcccgga tgcctcgtct tcagagataa tgataggacc atcgattggc cctgtcattc
     8341 cctggctctc ctttttcttc tcaatctcag tatcgatatt ctccgctttt gacatcgttc
     8401 ctccaaagcc ggacgtcttt tatactctgg cctgtaacac ttttcaaaaa agaaagtcaa
     8461 tctggcgaag cggtcgttcg cgacgaagat ccggtagcag aggccggaaa agttcaaagc
     8521 gagtggcgta agggaggggc agcctgggtt aggcttgcgt tgtgtgtttt gcgacgttag
     8581 gtaaaggcgg ccggacgatc ggcacagaat tacaacgagc ggaaaggctg gcacggagct
     8641 gtcaaccaga gattggttcg aaaacccatg gtcgggtcgg accggctaat tgcttgactt
     8701 atcgcagtcg gtttttgacg atgagccgct ctgcgacatc tcaaatcgca aatgtcaaat
     8761 gacagatgtg agatctgaaa tctgagattc cgaccgcagc gaggacttgg gccgcttgat
     8821 cgctgtgtca ccttgaactg cgaattcggt tctatctaga atcctgaccc aggctgttgc
     8881 agaaacgaaa gctcctcggg cgttgagatt cgcccaagcg cttcgtttct atgcgggaac
     8941 cggcggaagc ggctgatgat gtcgcggtgg cgcacggcga aatcgagata ttctgcaaac
     9001 gtgggcttct gatccgcgcc ggcttcatcg accagtttct gaaacaggct caccgctcgc
     9061 tgttgatcgg caagtaattc cgaatgctcc agcggcaggt aaaaaaatac cttctcgaga
     9121 gggcgcaagc tatgatcgaa gccggcgtca atgccagtgt gagcaagaga caacgctttg
     9181 ctgtcaaacg cgaaagcttt ggccgaatcg cgatagatac ttcggggaaa ttgatctgta
     9241 agcagaatta gcgcgagccg atcgtgcgga ttcaattccc aatggtctaa ttctcccgat
     9301 ccggccttaa tcacgcagct ttcaaaacgt cggcgaattt catcatcaac ttccgggttt
     9361 ttcgaccacc acaaccgtgc ccgttcatga gccgtgacag cggcatcttg caagtcggcg
     9421 ccgaaccaat attcgcggat ggtgtcggga gtttccatga ttgttgcggt gcttgtttag
     9481 gacgcggcaa atttaagcca gcgccaggat aattgcccct atggctaata atagggagat
     9541 tatcgaaatc agtcgaaatg ccgcaacctg tttgcgtagg acggtgaggg catcggtcat
     9601 cgccgccatt tgttgggcca tgctggccac gagctcggct tgcttccgtt cattcagctc
     9661 gagctcggcg accctatcct cgagcggctt agccccggat gattggtcgg ttctggtctg
     9721 ctgtaggagc tgcttagata aggataggat ttcgggaacc agcttaacaa tttgaaacca
     9781 gggtatcgcc attttttctc gtttcgttat tgggcttcag catctgtcat cgcttaaaca
     9841 gcgtcaatct aacatgcggc cgagcccatc actatctctt gatgatcaat tgatgatcaa
     9901 ttggccgcca tttggatcgc gtagtctcac tcgaatcatc tctggtttga cagttgcccg
     9961 acctgttgga taatctctcg ccgatcgact tgagggtgat caaaactcgc acgactaaat
    10021 aaggagatct ctgatgagca tcgattttgt cactaaccaa gaaatcattc tagcggcgcg
    10081 gcgcaacctc acacaggaca tttgggatta tttgagcggc ggcgccgagt ctgaaaccac
    10141 catgagacgt aaccgcatcg ccttcgacag tctcgcgctt tgcccgcgag tctgcgtgga
    10201 tgtctcgaag atcgacactt cgacgacttt cctggggcag aagctgcgaa ttcccgtaat
    10261 gatggcgccc atcggctcgc tgcaaacgat caccccggag ggcggtgtcg cggttgctaa
    10321 agccgcggcc gagttcggca cgatgaattt tgtcagctcg gtaacccagc cgagtctcga
    10381 ggaaatcgcc gcaagcacga agcatccaaa gatattccag ctctacattc gcggcggcct
    10441 ggactggtgc gaggaaattg tcgagcgggt gaaacgcgcc ggatatatgg cattgtgcct
    10501 taccgtcgat acggctcatt acagccggcg cgagcgccag atgatgaatc gttggttgcc
    10561 gcccaacaag cgcagtgaaa cgacgcgcca ctttcaagcg acgctcacct gggaaatgat
    10621 ggccgcgatt aaaaagatgg ccgggctgcc gttcattctc aaaggcgttg ctacggctga
    10681 ggatgccaaa atcgcgctcg atcacggggt tgacgtgatt tatatatcca atcatggcgg
    10741 gcgccagctg gatcatggtc gcggcacgat ggacgtgctg cccgagattg tccaagtcgc
    10801 gcgcggcaag gccgacatca tccttgatgg aagtattctg cgcggcaccg acatcctgaa
    10861 agcactggcg cttggatgca aagcggtcac catcggcaag cttcagggct ggggtctggg
    10921 cgcgggagga caagccggct tggtgcgtgt cctcgagctg ctcgaagagg agctgatcat
    10981 cgatatgggc ctgctcggcg taacgcgaat cgatcagatc tcggaaaaat atgtatgtaa
    11041 agcgccgccg gtatgctttc ccaacgagat gagcgcgttt gctcatctgc ctggcggcca
    11101 ggtgcgctag ctcaagcgca atcgccggcg ttgtccctag caaagattgg gcggggtccg
    11161 ttctcctcga atggcctttg tcgacggcct gttttttagt tcgacttgaa gcggtttccc
    11221 tgcaggaact ggaatttaac tcggttttgg ctggatcctg cctgcaatgc gctacccgtg
    11281 agcagcgcgt cctccgcaag ctccgctagg tgcatggcat gtgattgcgc atcgcaatca
    11341 tggataggga ttgtccatcg cagcttgatt catctgcgcg tgcagctcga tcatggtcaa
    11401 agcgcagtat tcgatcgaat agccggcgcg aaattctact tcctgctgaa ggacgcgttc
    11461 gaggacgttc ctatgaaaac cgccggtgac gattttctcg tcactcgcgc ctcgattgcg
    11521 gctctcttca tcggcttctt cgcggcgggc aaagtaaatc gcctgctcgt tagtaaagcc
    11581 gtacttcgcc gttaaagcgg tataccactc gcgcatccac tgggacagcg attcttcgaa
    11641 aacatgcaac ccccagagct cggcgatgga tccgtcgata aaaatcttgt ggcagaagtc
    11701 actgatggcg cgggccgccg gtaaataggg gtcttccagc atttcttttc ggctcagccc
    11761 caacgcttct gcagtctgca acagcagcag cacgtgtccc ggcgctttgg gagaaattaa
    11821 cttatcggcg accttggcgc agaaagggcc aaggagctcg aaattgcgaa cgaagaaagg
    11881 cagatgggtg tagtaagaga ccgcatccag accgtttagc tcgatcgaga aatggcccca
    11941 gtttttgaaa aactggcgga tcaccggcag gggtaaagtt ccgtcgcgca attgagccat
    12001 aaaagacccc ttgtgaattc tttcctgcga cgcttgggac acggtcgaat agagataatc
    12061 gacataggct tttgctgctt gagcgctcag cctctcaccc gcttgcggtt cttgcggctt
    12121 ggtcacgtgc tacctcttag aacagctctg ctgattgatc gatacggcgc gtttttcatc
    12181 gacgatcaac agtatcaata tttccggcga attggcaaag cctataaccc taggttagga
    12241 tatccgcatg ccaaccgttc caagcaaacg attagagaca tttccaaacc cgcatccgga
    12301 gcgcgactat gagatcaata tggaggcccc ggagtttact tgcgtctgcc ctcgcaccgg
    12361 tcagccggat ttcgcgacca tccgaatccg ctacataccc gatcagcttt gcgtcgagct
    12421 caagtctctc aagctctatc tctggtctta tcgcggcgaa ggcgcgtttc acgaagacgt
    12481 gatcaatcga atcctggatg atttcgtcga ggcatgccgg ccgcggaaaa tcacggtcgt
    12541 cggtgatttc aatgttcgcg gcgggattca cacgacgatc acggctggtt atccctcgct
    12601 ttaggccagt atcgtttctt aaaactgcgg cgagcgctga gccaacttcg cgctcccatc
    12661 cggcgctctg ccgagtcagc tcaaaaaatt ctccgcgcgc atgccgctgt tgataagctt
    12721 gtgcacaatc tgttcaccgc gcctacaact agttgcgtta aattgaaaat tttcagatca
    12781 attgttttca acaattctct gtcacaaaat tccagctcaa taatctcgcg acgaggcgaa
    12841 attgttcttg ctttttaatt gtggttgggt tcaaattcag aaacattttt cgcagcacaa
    12901 tatcgcgtcc gattcctctc tccggttttc atggcagcat tagaagacaa tctacggaag
    12961 gttccaccgc agaatcttga agccgaatcc tcggttctgg gcggaattct gctcgaaaac
    13021 gatgccatca acgtggtgtt ggaagtcttg cggccggaag atttttaccg agaatcacac
    13081 cgcaaaatct tccgcgccat gatcgagatt ctggaccgca acgagccagt ggatttgatc
    13141 acgctcagtg attttctcaa aggaagaagt gaactcgaag cggtgggcgg gacttcttac
    13201 ctggcatcgc tcgcggattt tgtaccgact gccgctaata tctcctacta tgctcgcatc
    13261 gtccgagaga aggcgatcct gcgcagcttg atcggcgccg ccactgaaat agcgacccgc
    13321 ggttacgaag aacagggcaa tgtcgaagaa tttctcgaca ccgcggaaaa ggtgatcttc
    13381 gacatttcgg aaaagaagat caaggcttcg ttcgtgtcag tcggagacat gatcaaagat
    13441 acgctcaaga cagtcgagaa gctgtatgag cgcaaggaaa tggtcaccgg cgttcctacc
    13501 ggttatgaag acctggacaa attgaccgcc ggtctgcaac cggcggatct cattatcgtt
    13561 gcaggacgcc ccggaatggg gaaaacggca tttgctctga atattgccac caacgcggcc
    13621 tataccggag tgggggcggc ggtcttctcg ctggaaatgg ccaaagaaca actggtgctg
    13681 cgcatgctct gctcggaagc tcatgtcaac agctccaaag tccgctcggg atatttgggc
    13741 gaacgcgact ttcctcagct ggccaaagcg gctggacggc ttcacgaagc gccgatctac
    13801 atcgacgata ctccggccat ctcggttctc gaactgcgcg ccaaggcccg gcgcctggtt
    13861 cgtgatcgtt ccaaaaagat cggtctgatc gtagtcgatt acttgcaact tatgcgcgga
    13921 atgggcacgg ccaataatcg cgaacaggaa atttcggaaa tctcccgatc tttgaaagct
    13981 ttggccaaag agttgaacgt cccggtcatg gctctatccc aactcaatcg gcgcgttgaa
    14041 gaccgcggcg accgccggcc gatgatgtcc gatctccggg aatccggcgc gatcgagcag
    14101 gatgcggacg taatcatgtt tatctaccgg gacgaggttt acaacaaatc cgacgagagc
    14161 aagaaggggg tggcggaagt catcgtcgcc aaacagagaa atggtcctat cgataccatc
    14221 aatttaacct tcctcagcga gttcacgcgt tttgaaaatt acaccgggag agatgacgac
    14281 tattatgacg aggccgacga agcataggcc aaaaatgcta acggcggcga agtgaaattc
    14341 aatttcgagt gcgcacgaag ttgaccatct cgaggctcat tgcgatttcg ccgttctgat
    14401 taagcacttc gacaaacgac cggatgatgc cacgatccga cttggagcgt gaaatgcggg
    14461 catcgataac cgtcacccgc actgacagcc ggtcgccggg aaaaaccggt ttgtcccagc
    14521 gaattgcgtc gcatcccggc gaacccaaat tggctatttt tgacaggtag ttttcggtat
    14581 agagccgcat catcagggcg gcggtgtgcc agccgcttgc gatcaggccg ccatagatgc
    14641 tcttctgcgc cgcttcttta tcgatgtgat aggacaacgg aacatagcgt ctgccgaact
    14701 cgaggacttc ttcctcatcg acaagcaccg agccgaactc atgcacggcg ccgggaacgt
    14761 aatcttcaaa ataacgattc tccggcggat ggcgaaaact gctcttgctc atagggcgga
    14821 gctcagcggc gggccaggat tgcgcttacc ttaactgcca cttcgtcggc ttttaagatg
    14881 tcgctgataa tcgctcctga gtcagcgccc gcccgccaaa cgctttgaat gtttgcttcg
    14941 gtaatcccgc cgatggcgac aatgggtaaa gcgactgccg ctctgatccg cgccagcatt
    15001 tccaagccgc gggccgtata gccggtgttt ttcgtagccg tgccgaatat cggcccaaaa
    15061 ccgatgtaat ctgcgccgtt tgcttccgct tctttcgcct gctcgaggct gtgtgtggaa
    15121 acgccgacga tcctttcgcc cagcaatttt ctgccgacct ctagcggcaa gtcttcctga
    15181 cccagatgaa ctccgtcggc agcgctaccc agggcgatat cgatgcgatc attgacgatc
    15241 aatttgcagt tcaatggcgt ggtcagctcg cgcgcgagtt gggcaagctg gaggaagtct
    15301 ctcgatgaca ttgctttggc gcgcagctga attatcttgg cgcctccgaa cagcagctga
    15361 cgcagaacag catcagcgga gctggtgccg atttgctccg gatcaagaat ggcgtacaac
    15421 gctggcagcg tcactctcgc ggacttgcgc gaagtcatct cacgaactct cttttttcaa
    15481 ctcgatacga aggtcggtga tctttttacg caaggtattg cgattgatgc cgagaatctg
    15541 ggcggcgcga atttgattgc cgcgggtttt tttcagggtt agttcgatca acgggcgctc
    15601 gatgcgctca atcacaagcg gatagaggtt gtctacctcc actccttctg tacggcggaa
    15661 gtagtcctca agtttgtgtc gaaagacttc ctcgagagag agctgatcga tttccggggc
    15721 cgcctccgac tgcttgtttt gtaaattgaa gtcctttgga aataagatcg ggccggacgc
    15781 cagtacggca gctcgaatca aagtattttc caactcacgg acgttgccgg gccatggatg
    15841 ttcttccagc agccgtaacg cgtcaggcga aaccccggaa atttgaatgc ccatctcgcg
    15901 attgatctta tcgataaagt aaccgatcaa gagcggaata tcgccgcgac gttccctgag
    15961 cgccggcaga taaatgggaa tcactctcaa gcggaagtag aggtcttctc ggaagcgctt
    16021 atcgtgaacc gctttgtcca agtcctgatt ggttgccgca agaatgcgga cgtcggcctt
    16081 tagcatctct cgccccccca cccggctgta ctcgcggtct tgcagaaccc gcaggagctt
    16141 ggtctggagt tcaagtggaa tgtcgccgac ctcatcgaga aacagagtgc cgccttgcgc
    16201 caactcgaac ttgccgatac ggcggtcgag agcgccggtg aaggcgcccc tctcatggcc
    16261 gaagagctcg ctttccagga ggtcgcgcgg gatcgcggcg cagttgatcg cgataaatgg
    16321 ctggttccag cgcggcgaat tataatgaat cgttttggcg acgagttctt tacccgtacc
    16381 gctttcgcct tggatcaaga tcgttgcctg ggtatcgaca acctggccga tgattttgta
    16441 gacccgttgc atgctcgagc tggtgccgat gatattgaca cccggttcga atcgcttctt
    16501 gacttcttct ttgagctcgc ggaaatcacg ggtgagcttg cggctatcca gcgcgcgctt
    16561 aaccaagacc agaacctcgt cgagatcgaa cggcttggtg atatagtcga aggcgccgct
    16621 tttcatcgcg cctatggcat tccgcatggt gttttgcgcg gtcatgataa tcatagccgt
    16681 gtcgatgcca tcatctttgc tttccttgag caccgtcaat ccgtcaacat caggcagctt
    16741 gatatccaaa agcgcgacgt caaaattgca gtcgctcaac gattggcgcg cgccggatcc
    16801 atccacggcg gtgtggaccc agtagccttc ctcctccagc gccttcttca agacgaagcg
    16861 cagggactct tcatcttccg caaccagaat cttgccttgg tccatgttaa tcggctacca
    16921 ctaacgaaac tttaaacgtc gacccttttc ccgcgaagct ttcgacccga ataattccgc
    16981 cgtgttctct tataatccaa tagcagacgg cgagtccaag cccagtgccg ctgttttttg
    17041 tcgtgaagaa cggcgaaaaa atatacggca ggtcttcctc cttgatgccg ctaccttgat
    17101 cggcaatctc cacccagata aatctgctcc gttcggcgcc ctgcccacga acatgcctat
    17161 cggtctcaag gcgtgtcgta atcgtcaagg ttcccgagcc gttcatcgct tgatagccat
    17221 ttttcaccag attgagaaag acttgcgtga gccggtttcg atcaccccgg attggcggga
    17281 gacttggatc gaacagtttt ctgatattaa ccgttggcgc gtcctgggtt ttcaattcaa
    17341 gcagcagaac atcttccaga acttgatgaa tgttaagcgg aacaatttcc agtttcgccg
    17401 ggcgggatag atcgagcagt tgctcgatta gcagattgac ccggtcgacc tctcgattca
    17461 tgatgttggt gtactcgatc agtgaagaac cgggatccaa agaacggcgc agcagctggg
    17521 cggcgccttt gatgccgcca agcggattgc gaatttcgtg cgccaacccc gccgccaaag
    17581 tgccgaccat cgctaaacgg tctgcgcgtt ttaaatcttc ttcgaattcc ttgtgctggg
    17641 tgagattgcg caccagcaag atactgccaa gacacttgcc atcggtctcg accagcggag
    17701 agacggtaac accgaccggc accctctttc cccaacggga caccaggtca ccctcgcctc
    17761 tggcgctttc ctgcagtggt gggaaactct ttttcaccaa ttcgagcagc caaggctctt
    17821 tgcgaaaaat tcgtaggaac gacaggctca gagcgcgcga agcggccagg tcggtcaaga
    17881 tttccgccgc ttcattgaag aaagaaactt tgccgctgcg gtccagcgta atcaccccgt
    17941 cttcaacgct ggaaagaatt tgctcccacg agatttttcg ctcaacggca gcggacggcg
    18001 actcgattgg tttcatgttt tggatgggcg ggcgtggaaa agcatagcaa ataaaccttg
    18061 ttaggcaaat gttagccgtc cgtggggctt gcattgatga gcgccgccgt ggtagaaaaa
    18121 aatacacttt tagagttgcg actcgaagtg ggctctgccc gctttttttg tttctggggg
    18181 tatggaacta gatcgaaccg tctcgcgaat atgggaaatt gcgacccagg ttgcggatgg
    18241 ccaagggatg gaagtcgtcg acgtagagct ccggagcgaa ggcagccgag gcggaagagt
    18301 tttacggctt tatctcgata aagagggcgg tcccaacatc gacgagctgg ggcgggtcag
    18361 ccgcgaactc agcgagcttc tcgacgctca agacattgta gaaggatctt acaccctgga
    18421 agtatcctcg cctggtatta accgtccact caaaaagccc gagcactttc gacgattcac
    18481 cggcaagcgc gtccgcatta gaactcggga tatgatcgaa ggacggcggt cttttttagg
    18541 cactctcagc gaagtaaccg gggagaagat taaagtcgaa gttgaaggca agcaatacga
    18601 gattccgttt acaacgattg aaaagtccaa ttacgagcac gattggagcg cgtaaatgca
    18661 gcaggatctg aatcgggtca tcgagcaggt aagcaaagaa aaggggatcg acaaatcgat
    18721 ccttatcgcc gccctggaaa ccgccatggt ttcggccgcc aaaaaaacct tcggtcatca
    18781 gcggaagatc gaagcccagt ttaatccgga aattggcgag gtggagcttt tcgaagccaa
    18841 aaccgtggtg gagaacgtta ccgacgccgc ggtcgaagtc agcttgaacg aggccaaaga
    18901 gagcttggat ccggatgctg aaattggcga tgaacttttg tctaagctgg attccagcat
    18961 gttcggccgc attgccgctc aggcagccaa acaaaatatt gttcaacggg tgcgcgatgc
    19021 cgagcgcgag attatctaca atgaatttaa aggccgcgaa gggcagctcg tcaatggcat
    19081 cgtacaacgt ttcgagcgac gcaacatcat cgtcaatctc ggccgcaccg atgcgatact
    19141 gcccgagaag gaacaggttc cccgagaacg gtatcggcag ggtgatcgga tccgcgccta
    19201 tatcgtcagc gtggagatta ccagccgcgg accgcaaatc gttttgtcgc gcacccatcc
    19261 gggcatgttg attcaactct ttgctcagga agtaccggaa atatacgagg gtattgttga
    19321 agttaaagga gccgcgcgcg agcccggcgg ccgggcaaag attgcggtca tatccaatga
    19381 ttcggatgtc gatcccgtcg gcgcttgcgt gggcatgaaa ggcacccgtg ttcaggcggt
    19441 ggttcaagaa ttgcgcggcg agaagatcga cattgtgcac tggataccgg accaggcgga
    19501 atatgtttgc cgagctttgg cgccggcaaa ggtgtccaag atcattatcg acgacgacga
    19561 gcacagcatg gaagtgattg tgccagacga tcagctatcg ttggccatcg gcaaaaaagg
    19621 acagaacgtc cggttggccg cgcgtctgac cgggtggcgc atcgatgtcc gcagcgaagc
    19681 ggaggctgaa gaagagacac gccgcgcgcg catgtctatc ggcgcaattc ctggcatcaa
    19741 cgattttgcg gcggagctcc tttatcaggc cggattcaaa tcggccgagg aggtttctga
    19801 aagcgaactc gaagatattc tcgatgtcga aggaatcagc aaagaaaaag ctgaatcgct
    19861 gcataaatcg gctaaggaat acgttgccga gaagcgctta aaagcagagg cagaaaaggc
    19921 caacgcggct gagcatacac atgaaacggc accgtccgag accgcgtcgc ctactgaaac
    19981 cacggcggcg cccgaggcgg cggaagggat taccaggggt tgataatcgg cgagtgtggc
    20041 aagcgatgaa cgtcatccgc agcggacgtg cctgggctgc ggatgcaggg acgatcagaa
    20101 aaacctcata cgcatggtca tcggtgcagg cggtgagttg atgattgatc cgagaggccg
    20161 ggggcgcggc ggttaccttc atcgagggca tgactgctgg caggcgttcc tcaggcgcaa
    20221 gaacactcac cgagcattcc gtgtgaacgt tagcaaggag tcaaagcaaa agcttattca
    20281 ggtcttgaat cagggttatc gggagtaaca gatgggaaaa atgcgagtcc atctactggc
    20341 aaaagagttg gagattgaga ccaaagagct catcgctcag ctcgagcgac tgggaatcag
    20401 ggggcggaag gctcaaagct cgctcgagga tgaggaagtc actcgtgtgc gcgctgctct
    20461 cgcgatgcag gaaaaaccgc acgtgcacgt cggccaagag aaagttgttg ccgatcgagt
    20521 tgtcacggcc gatgaccaaa gctcaggcga gatacaggcg cgcgagacca tcgtagagcg
    20581 ccgggttcgc gccaacgtga ttcgtcggcg gaccagccga gtcgaggttc cgacggaaac
    20641 tcatgaggcc gagacaagcc cagccgaagc ggttgcaacg cccctcccac catcagctcc
    20701 taacttcgaa gacctagccg ccaccatgcc ttcctatgaa gatgattcat cgttaaccgc
    20761 gatcgcgccc gagagcgata cttcgcatca tgaggaagcg gaagaaacgc caggtgaaat
    20821 cgatgaggcg gctccaatgg aggagccgca agctcaggca gttacggcac cggatcgaca
    20881 gcccgtcccg accgctcctg caccgccgcc cgctgcgccg ccagctgtcg caccggctgc
    20941 gacgccgaag gaacccctac ggggcgcgcg agtgcttggc cgcatcgatc taaagcgcac
    21001 ggtcaccatt gcaccggcgg cgccagcagc gccacggcgg ccagtgccag gatcgcccgg
    21061 agttccgccc gctgccaacg acgcaacacg gccggcgcgc gatggcgaag cagccaagcc
    21121 tgcaactggg gaagccgaga agcctaaagc cggtcgtcac aagaagcgcg tcgtcaaaaa
    21181 acaagatgtc ttggaatttc gagagaagga gctacgctcc gggcgcattc cgaaaaagaa
    21241 gcgcgcgctc ccgggcaaag aacagagaaa aaccgaaatc accgtgcccc gggccagtaa
    21301 gcgcgtgatc cggatctcag aagtcattac cgtgggagat ttggcgcgcg agatgggcat
    21361 caaggccggc gacgtgatca agaagttaat gagtctcggc atgatggcca gcatcaatca
    21421 ggttctggat gtcgacaccg caacgttggt ggcggccgat ttcgattatc aggttgagaa
    21481 tgttgcgttc gatgccgaga gcatgctgga agtcgagcag gtggaagaag actcgatgct
    21541 ggtttcgcgg cctcctgtgg tgaccattat gggccatgtc gaccatggca aaacatcgct
    21601 gctcgatgcg attcgcagca cccatgtcac ggcccaggag catggcggca tcacacagca
    21661 catcggcgcg tatcacgtta aggttgatgg tcgcagtgtc acttttttgg atactccggg
    21721 tcacgaagcc tttacggcga tgcgtgcccg gggcgcgaaa gttaccgaca ttgttgttct
    21781 agtggttgcg gccgatgacg gcgtcatgcc gcaaaccgtt gaagcgatca atcacgcacg
    21841 cgcggcggag gtgccgatta tcgttgcggt caataagatc gacaagccgg acgcagatct
    21901 ggagcgagtg aagcggggac tctcggaaca tggtctggtc tcggaagact ggggcggcga
    21961 caccgtcttt gccccggttt cggcaaaaac tcaggaggga attccgcacc tgttggaaat
    22021 gttgcttttg caagccgata ttctcgagct tcgagccaat cccgacaagc tggcccgtgg
    22081 aaccatcgtc gaggcaaagt tggatcgcgg tcgcggaccg gtcgccaccg ttcttgttca
    22141 ggagggcacg ctgcgcgtcg gcgattcgtt cgtttgcgga gtattccatg gaaaagtgcg
    22201 cgcaatgatc aacgatcttg gtcagaaggt cgagcttgcg ccgccgtctt cgccggtcga
    22261 gatcctcggc ttgcaaggtg tgccgcaggc gggcgattcc ttcatcgcgg tcgcggacga
    22321 gtccaaggca aggcaggttg ccgaacatcg tcataccaag cagcgagaaa ccgaattggt
    22381 caaatcgagc aaagtgtcgc tcgaagaact ttacgatcag atcaagacgg gcgatgtcaa
    22441 ggagctacgg gtggtggtca aggccgatgt gcaaggttcg gttgaggcgc ttaccgatgc
    22501 gctgacgcgc atgtccacga ctgaagtgaa gttgcgcgtc cttcatggtt ctgttggagg
    22561 cattaccgag agcgatatac ttttggccgc agcgtccaac gccatcgtca taggcttcaa
    22621 tgtccgtccc gaatccaaag gtcaggcttt ggcggcgcag gaaggtgtag acgtgcgcct
    22681 ctatacggtc atttacgaag ctgtcgccga cgtccgggcg gcgatggagg gcatgttgga
    22741 accgaccttc cgcgagcaaa cccaaggccg agtcgagatc cgtcagattt ttaacattca
    22801 aggtgttggc actatcgccg gctgttatgt caccgagggt aagattcagc ggggtaattt
    22861 agtgcgaatt ttgagggacc atgtcgttgt gcacgaaggc aaactcgcaa gtctgaaacg
    22921 cttcaaagat gatgtgcggg aagtttcggc gggctatgaa tgcggtttat cgatcgaagg
    22981 ttttcacgac atcaaacaag gtgatgtggt tgaggcttac gagcgaaccg ccgtggtgcg
    23041 gcgaatcagc ccggtcgcaa gcggtcgtga ggcgtcgcac tatgccggga gatagcgatt
    23101 ggccgtaagc acgatcgcga gccgactccg cgtgccgtcg tcggggtact gaaggtcaat
    23161 cttctacttc cggagaatca ctcattaaaa ggcaagcgcg gcgtgctcaa acgtattcag
    23221 gcgcgggtcg ccaaccgctt caacgtctcc gtgaccgaat gtggggatca ggatttatgg
    23281 caaagcgcgg ttctcggctt tggtttgatc ggcacccata aagaaatcat tgagaaaact
    23341 cttaacaaga tcgttgactt catcgatgag atcggactgg cccaagtcgt cgattgcgat
    23401 atcgagtttt tctattgtta aaattttctg ctcatggatt atcaacgatc agaacgcgtc
    23461 ggcgaactgc tcaccgaagt tatcgccgag gtcttacgtg aggacattcg cgacccgcgc
    23521 attactgcgg tgacgattac cgctgccaaa gtaaccaagg atttacgcca ggcacggatt
    23581 tattttacca ttctaggcgg cacggccgag aacaaacact cagttctcgc aggattgaag
    23641 agcgcgttgg gattcatccg ttccagaatc ggcaagcagc tcaaacttcg tttcgttccg
    23701 gctattgaat tcttttacga cgaatcggtc gatgaggcgg aacggatcga gaaccttctc
    23761 cggcaagtaa agcaataatt cgactctcgg cgaaacgccc gcaggctcat cttcagtttt
    23821 cccaacgtgc aaaaatcgat gacaaaaagc ggtcttcttt tggtcgataa gccggaagga
    23881 ccttcttccg cccacatcgt gcacagggca aaggtcgctc tcggcgccaa aaaggtaggc
    23941 catctcggca ccctcgatcc atttgcctct ggattactgc tgttggggat caacgaaggc
    24001 actaagatct cggatatttt tttgcgggcg ccaaaaagtt actgcggtgt cttgaccctg
    24061 ggagtggcaa cggattcgca ggattccacc ggggtggtga taaagacgct cccggtgccg
    24121 tcgctcgccg acgaggatct ccgtcttttg gagcaacggt tccgtggcga gcttcagcag
    24181 acgccgccga tgttttcggc tctgaaacgg gacggggtcc ggctgtacaa actggcgcgg
    24241 cagggcaagg aagtgccgcg gcagccgaga acaatccaca tcgattccat ccgcttgcgt
    24301 cagatcaatc gcacggaact tagcatcgac gttacttgct cgcgagggac ttacgtgcgg
    24361 accttggcgg cagatttggg tgaagctctg ggttgtggtg cgcatctaaa aagtttgcgg
    24421 cgaatcgctt gcggccatct gagaatagat caggcagtga ctctcgatga gctcgagcaa
    24481 tttaggacac gagcgcagag cgcgatgatt tcattgagcg acgcgcttgg ccatctgagg
    24541 gggattagct gggatcagcg ctggctggcc cgcctccgat tgggccagca ggaagcttta
    24601 ggtcagatcg gcaaacccct cgaaaaacag gaactagtcc gtttattgga tgcccgtggt
    24661 gatcttgtgg ccgtgatcgg ctggagcgac gacattcctg gcgacggttg gcggttgttg
    24721 cgggtgttca aagaatagcc tcggtgaacc gaatgaccgc gatgcgacgc cgacctccac
    24781 gcacgcgcgc tctcgtggtc ctttgatcct cgaaagttga tttttaaaat ccagctggcg
    24841 agcatttcat cgctatcagc ccgcgctctc cgactcagtt gacattgccg cttttgatcg
    24901 gatagtttta aagtgaacgc agtgcttgcc ggtctggctt cttattcaat ttgcgtctag
    24961 ccggaccaaa gattccgcag gttctagatg actcgaacat cacgtcttgt cgaaagggag
    25021 aattttggcg catgagtact tcagcagtaa aggcaccggc aaatccgcat attcaaaagt
    25081 gggtcaagga gatggcagcg atgtgccaac ccgacaacgt ctattggtgt gacggttccg
    25141 aatcagaacg ggaggaactc acacgagtcg ctgtggaaac cggcgacttg atgcccttga
    25201 accaagacaa attgccgggt tcgtatttgc atcggagcgc gctcaacgac gttgcccgca
    25261 ccgaaaatct aaccttcgtc tgcaccccaa cggaagagga agccgggccc aataacaact
    25321 ggatggcacc ggctgagtct tatgaccgtc ttgccaagat ttttaccggc gccatgcgcg
    25381 gtcgaacgat gtatgtcatt cctttcctca tggggccggc aagttccgcc ttcagtaagg
    25441 ttggggtgca actcaccgat agcgtttatg tggtgttgaa tatgcgcatc atgacgcgaa
    25501 tgggcaaagt cgctctcgac catctcggcg gttccaacga ttttacccgc tgtcttcatt
    25561 ccaaagccga tctcgatatg gaccggcggt ttatctgtca ctatccgcaa gacaatacaa
    25621 tttggagcgt gggttctgga tacggtggaa acgccttgct tgccaagaag tgtctagcgc
    25681 tgcgaatcgc cagtaaactg ggtcaaaaag aaggctggct ggccgagcac atgctgatcg
    25741 tcggcatcga aagtccggat ggcgagattc actacgtttg cggcgctttc ccgagcgctt
    25801 gcggtaaaac caatcttgcc atgttggtgc cgccggcgag catgaaagga tggaaaattc
    25861 acacgatcgg cgatgatata gcctggctca gggtcggttc ggacggtaga ttgtgggcga
    25921 ttaatcccga agccggtttt ttcggcgtag cgcccggtac cggatctaaa accaatccca
    25981 atgcgatgca aaccgtgcaa cggaatacca tattcaccaa tgtggcactc cgatcggacg
    26041 gcacagtgtg gtgggaggga cacgatgatc cggtcccggc caacgctatc gactggcgcg
    26101 gcaaggcttg ggatccgaag agcggtcatc cggcggcgca tccgaactcc cggttcacag
    26161 tatctctcaa acagtgcccg acttattctc ccgaatggga aaaaccgcaa ggggtgccga
    26221 tcagcgcaat cttattcggc gcccggcgta acaaagtggt ccctctggtt tatcagtcct
    26281 tcgactggca gcacggcact ttcctcggtg ctacgttggc atcggaaact accgctgcgg
    26341 ccactggagc cgtcggggtt gtgcggcgcg atcccatggc gatgttaccg ttctgtggtt
    26401 acaacatggg agactactgg ggtcattggc tgtccatcgc caagcgagcc accaatccac
    26461 caaaaatatt tcgcgtcaat tggtttcaga ggaatgatca aggaaaattt atttggccgg
    26521 ggttcggcga aaacttgcgg gtgcttcgct gggtcatcga gcgtgcaaaa ggcggtggcc
    26581 aagccgatga gacggcgatc ggttatgttc ccaagacgtc aagcctcaac ggcgatggac
    26641 ttggcgtttc gaaatcggat ctcgagcagt tggtaaccgt cgatcgcgat gcgtggaaag
    26701 caaatctaaa aagccaaggc gagtatttcg ataaattcgg tgaccaccta ccggcgggca
    26761 tcaaggaaga acacagagcc ttggccgaac gcctgaaagg tggctgattg gagcaagtca
    26821 tggaaagagg acatcacgac atcggcggac tacccggagc cggaccgatt gacaaatcgc
    26881 agcacgagct ttcagattgg gagattctgg cagacgcgat caatcaagcg ttgggcgcaa
    26941 agggagtcaa gcgaaccgat gaaatgcggc gcgcgcgcga ggagatggat tccgaggcct
    27001 accgggaaat gaattattat gagcgttgga ttgccagcat ggaagcaatc ctaattgaaa
    27061 agggcatctt aaaaaaagat gaaatcgaca agaaggtcgt tgaattcgaa aaaaaatggg
    27121 gagagccatg atccacccgg cgacgggcag atttcggccg ggcgacgccg tccgagtgct
    27181 aaacgagcat cgtcccggac acatccgcac gccgttttat attcgcggta aaactggctg
    27241 gatcgaacga gtttatggcg acttcctaaa cccggaatcg ctcgctttcg gcggtgacgg
    27301 attgccgaga cgcacgcttt atctggttgc gttcaaacag ggcgatgtct ggggagcggc
    27361 gcaagcgccg acagacaaac ttttcgttga catttacgac cattggttgg aacccgcaag
    27421 ctacggagga cagagagatg agcacatcag gtgacgatca cgatcacgaa catccgcgga
    27481 atgaacttag tgcgcaggct aagcggatct atgcgatccg cgatttactg ctggagaaag
    27541 gcgttatctc tgagaaggat attcaagatc aaatcgagta tcaggaagcg cgttctccgg
    27601 ccaatggcgc aaagctcgtg gcccgggcct gggtggatcc ggcgttcaag gcacgtctga
    27661 ttgccgaccc caaagcggct tgcgctgagc tgggaatcga cgcgacggcg ctcaacgaat
    27721 ttgtcgtttt ggagaatacc aaaaaagttc gccatatggt ggtttgcacc ctgtgctcct
    27781 gttatccacg gccgatcctg ggacgcccgc ccgattggta caagagcttc aactatcgcc
    27841 aacgctccgt aaacgatccc cgtggcgtga tgcgtgaatt cggtcttgtg ctccccgacg
    27901 atgttgaagt gcgggtgcac gacagcacgg ccgatatgcg ctatctggtg ttgccgctgc
    27961 gtccgaaagg gacggaagga ctgagcgagg tggaactagc caggctggtt acacgagaca
    28021 gcatgatcgg agttatggat gcgctgccga aattgccgga gacctaacca ctcttccttg
    28081 ttgttttgat gttgacatac gctcaggctc cgcgcgccat cggtcaagat ttacagaacc
    28141 atggcgcgct ggcttacgag atcaacgtca accaagccga gtatgtgttc aatgtgattg
    28201 cgacctaccg ccgccggaca atctcctgtc tttttgttat acgaggaaaa ttctcgagca
    28261 aatagacttc atgggtaaat tgaaacggac cggcatgtcc tctagggaca cccgaatcgc
    28321 tttctaacac tctccgtact gtgggaatcg tatgtagata gtcaacacgg tgcgctactg
    28381 cgtgtcactc attacgaaac gcagggacca gagagcgcat accgggttga atttgccttg
    28441 gccaaatgga gcccgggtgg agaagactct gtcgaagcaa gcggttgctg agactactaa
    28501 ctccttgcaa caggcgaaga agtgaagacg tccgcgataa ttcggcggcg cgactaaacg
    28561 aacgttttcg gcgtagtgat tgatcctccc gaaccctgac aaacaagaat ttgtttgatt
    28621 tttccgacca ctattcgtac catttctctt cccaatcata actccgttat agggatttct
    28681 gggaagctat gagaaggctg gcacggctgc attcggtcaa ttattcaacc gctcttcgat
    28741 gtattggtca ggatctcgaa agccgaggat tgcgcaccct tgatatcgtc gcggacaatg
    28801 ccggctacat tgtgctggcg ggttaccaac ctcctccagc gccgatgccg gtgagccttc
    28861 aatattcgcc ggctgatatc ctcgaactcg atcgtaccgg ccaggagcaa cgcggaacat
    28921 catcagcggc aaaagagttc gttacgcttg ttcagattct ccgagccatc ggtggatatc
    28981 tggataaaaa tcaagcgcgg ctcatccgca tttcgaacag tgaagcgccg ggcagcactc
    29041 ctgccttttc agtcgaatac gagaccaagg agggcgagcg agtcgtagac gatcgagacg
    29101 gtactgccat ttatgatatg tgcgtcagca tgtacaaaca gcgccgtaaa gttgatcatt
    29161 cggcgaagct ccccagccac ggacgctaaa atcacggcaa cccaatgatt aagcgcgggc
    29221 gagaaataca atccgttttg tgaggcgaag ttggaagatc taattgattt ttactctttg
    29281 ctgggtctta aacgaaacgc cagcgaagca gaaatcaaac aagcatatcg caagatggca
    29341 tttcgctatc accccgaccg gaatcccgaa gataaacagg cggcggaaaa attcacccaa
    29401 gtgctacagg cgtacggtat tttatcggac agcagcaagc gtagtatcta tgaccgggcg
    29461 acgcgcccac ccggtgaaga agatcctcag gaagagaagc cacagtcgga gcagtttggc
    29521 gaacacattc atcaaggatt caaccattcc tatgacttca aaggaaaggc tgagtcgggc
    29581 tccaaaactg agccgcaacc taagtgccct caatgctccg cgctcggcac ggattacatt
    29641 gtctcgcgta aaggcggcgt tgggtcatcc cgcggcaaac aatttgtact tgcgccgttt
    29701 agcgtcgttt tctgcagtga atgcggacac gtctacgggg tgatggggca gtcggcttaa
    29761 gatgcgtgag ggcccgtagc gatcgctctg cgtcaatgca tgatcccgcc gccttccact
    29821 accagtaact gtccggtgat gaaatcgctg tcggagctag ccaaaaatac cgctgtcccg
    29881 atgatatcgg ccggtgtttc gatgcgaggg aaagcgcgct taccggtact cccttgccga
    29941 cgctcgatca cttcatccgt gaccgaatcc tcgctcatgg tggagccggg ggtcatgcaa
    30001 ttgacattga tgttccattc tcctaattgt cgcgccatga ctcgcgttag cgcgaccacg
    30061 ccgccttttg ccgtcgtata atgcggcatg ttgccggagc cgctgaaaaa cgtgcccgag
    30121 gcaatgttga tgattttgcc gctgcgttgc cgcttcataa tcgggatcgc cgccttgcaa
    30181 cagaggaaaa cgcctttcag gttcacttca ataactcggt cccactcagc caattcgagc
    30241 tcttcgaccg gccctttcca aagcttctcc gtgacgtaga tcgccgcatt gttgagcagc
    30301 acatcgatcc ggccaaactt atcggccgtc tcccggatca aagcttcgac atctaaatag
    30361 tttctgacgt cagttgcgcg cgcccatgcc gcgaaaccgg aatccgtcaa tgcttgggca
    30421 acggtcttgc cgccttggct atcgatatcg gcgatgacca cctcggcccc ttcttcggca
    30481 aacctgcgcg cataggcttt gccaatgccg tgggcggcgc cggtaatgag gacgactttg
    30541 ctttgcaatc tcatggactc gccgtgatga agattaaatc attcgatgct gcaaaccgag
    30601 cgaattcagc taaagccagg cgctttgccc tggaacaagt ttttcagttg atcggcagga
    30661 aaacgcagac cgacgtagaa cgtgccgaac aggccgtaaa tcgcactggc ttcatcgaaa
    30721 cgcatctcgt atacgagttt tttaaaaacc agcggatcat cggcgaagag atcgaccccc
    30781 cattcccaat cgtcgaaacc gatggagccc gagatgattt gattgacgcg gccggcataa
    30841 tggcgtccga tcatgccgtg ctcgcgcatc atccgttgcc gctcctggat gggctcgttg
    30901 taccaattcc tcgattcgcc gcgaaactta ttcatcgggt aaaaacacag ataacggcgc
    30961 tgcggaattt ccggccacag ccgcggcgcc gccgcttgcc gctgcttggc cagttcggct
    31021 tccacttctc ggttccactc cgccgtgccg ggccgtagac ctcgggccat taggtcggtg
    31081 tacagtttta ccgaagcttc atagaggcca agctcgacaa ccgaaagata ggatgtcatc
    31141 ggctcgagaa aatcgagcag ctttaattgc gcgagctcga actcggcgcg atttaacgcg
    31201 tccagcgttc tgcgaaaatg caccaacatc agatcgcctt tgtggccgag catggcgaac
    31261 aagcccgtgg cttcttgctt gttgtcttcc atgtcatgaa atgtcgctat ggcgtggtcg
    31321 agagccgcct cttgctcggc agcgctgagc gcgcgccaag cggcccagcg cacgcgaaac
    31381 atctgatgca gtagataaga gccgtcgagg gtcagcggcg ttgccggtag atcgcctgcc
    31441 agtttgttca agggttgttt catatcatcg agtttatcat ctgctcatcg accacagctg
    31501 atatgaatgg tcatctcgca tcaaggtgcg ctggtgcttg cttgctccgg tttgatgacg
    31561 cctagccgcg tacgtagcgc tctaaatcca cgcttagcca cgcgaaagat cttgggcaca
    31621 agccaaacgg tcagcgccag aaaaacaaat actaacgcaa gcatcagcca tggatgattg
    31681 aagattatcc aaatgccgcc caaaactacg acatcttcaa cgaagctggc gatccaattg
    31741 ctaaatggct cagggctgga gttgatcgct agccggctag tcgctttggt gccgtgcgct
    31801 gccaaagcga tgctgccgcc agccagcagg gccgcgaatt ccaagcccgg gctcatgtca
    31861 ccgagcgagc gcgctgctag gatggcgccg gcaggtactc ggatgaatgt gtgtaagata
    31921 tcccaaccat tgtcgacata ggggattttg tcggcgaaaa attcaatcgc atacatgacg
    31981 cacgccactg cgatgacaat gggattagtt agcacatcaa ggctctgtgg caactggatc
    32041 atttcgaacc gacccgccag ccccagagcc gccaccgttg catacaggtt gatgccggaa
    32101 gtccaagctg ttcccactgc caggctcaat gcttcgatgc tgcccataat gcatccggat
    32161 caccgtagcg cgagagcggt gatcgtctga tctacgatcg tcgcagatgg agtctttttt
    32221 gaattctaaa ttttaaattt actttcgatc cgatctttga attcactggt aaacgcattg
    32281 accggcggaa attgatccat ccatttaaat ggccggcagg cgtcgatcag cacccggtca
    32341 gaggtgtagc cgcgcggtga tctttcctcg ggaggcaatg ccggctcaca tacggaggcc
    32401 caaacgcctt tgacgacatc gaggccttcg cgaacgtgac agcgggtggc aatcgcccag
    32461 atcacatcgg cgggattggc gatatcgata tcgtcatcga ccactacaac gaatttaccg
    32521 ccgtacgctc ccgcacgagc gcccgcggca accaacaagg cctgcttcga atggcccgca
    32581 taacgctgct ttatggagat gatcgtaaac ggccctggct ggccgccata gacgaaaccc
    32641 cagacgcctt tgatttccgg aactccggct ttctccagtt gttcccaaag cgtcgccgcg
    32701 cccaacggaa tgggcaggta gcttgccggc ggtttgagcg ggggcgcgcc gagaatgata
    32761 ggatcgtttc gataataaat ccgtttaacg ttcatgatcg gggtttcacc gacattctcc
    32821 tcagtgaagt aacccggcca ctcgccgaac ggcccttcct tgggcatgtc ttggtctctc
    32881 atgggcggca gttcgccttc aacgacgatt tccgcgttgg ccggaatcgg cagcccggtg
    32941 accgcgccgc gcgctaccgg caccggtgcg ccctgaagat aaccggcaaa ttcaaattcg
    33001 ttgacgtcgg gcggcaacgg catctgagcg gcaaggaaga tcgccggtcc ctgacagaat
    33061 gtaatggcga tcggtaatgg cttaccggca tcgcgggcac gttcaaacgc caagcggccg
    33121 tgcttgccca tgttcatctt aaccgaaaga gtattcttat tctggatcat cacccggtac
    33181 gtgccgatat tgatcttgcc ggtctccagg tctttggtga tcaccccaca gcccgtaccg
    33241 atataaggtc caccatcgag atcgtgccat ttggggactg gaaatttata cagatcgact
    33301 tgatcctcaa tcatttgatt ttcaaacacc ggaccgcctt cgacttgctg caccggcacg
    33361 ggtttgtagg cagccaattt tttacgccat gcgtttagat aatcaacgcc cttgagctcg
    33421 ggatcgatgc ccatggccgg cccagtgcgc ctgcaggtgc gaaaaacatt ggagatgacg
    33481 cgaaagcccg ctggataatc tttgaaattg ctaaacagca gcgccgcgcc ttctttctcc
    33541 gccgcccgct ccgtaagcgc gccaacttcc aagtcaagat cgacgccctc gagtcttcga
    33601 agttcgccca tctcttccag caactccaca tatcctctca tgtcggtcac atcggtcatg
    33661 ctgtcctcct catatgcggt catactattg tggtggtcag agctagtcaa atgagcgtca
    33721 aattcgaatc tggccgccta ttggaactgc caggatgaaa ctcgttcgcc gataatttgg
    33781 cagatcagaa tcatctttgc gccttcgatt ttcatcgaca ccgcgcgctg acctctcttg
    33841 cacataggac ggcacccatc cttgtcacgt gcttccgcga ttccttgaag tcaaagctga
    33901 cttcctttac ggttagagca tgcggcggtt attctctaac ttgactcttg ctttcttcct
    33961 gctcgccgca tccgcacaag actcttttgc cgctgttcaa gagtttttgc tcgaaaatgg
    34021 tctcaagatc cttcttctcg aggatcacaa gagtccagct gtaacctttc aggtctggta
    34081 ccgggtcggc tcgcgcaatg aacgagacgg caaaagcggt ttagcgcatt ttctcgaaca
    34141 tatgatgttc aaaggcacgc caaggaccgg gccggaagag tattctcaaa tcatcgccaa
    34201 aaacggtggc caaaccaatg cgtttaccac tgccgatgtg acggtttact tcgccactat
    34261 gagccgggac aaaatacaca ttcagctcga actcgaagcc gatcggatgg tgaacgcact
    34321 cctgggtgag gagtacttcg aagcggaaaa gaaagttatc caagaagagc gacgcctgcg
    34381 agtcgacgac aatccagctg cggcgctggg ggagctggcc ggcgccatca cctacatggt
    34441 tcatccctat cgccgtccgg tgatcggttg gatgcacgat atcgaaaacc ttacacggca
    34501 ggatttggtc gatttctaca agctttacta ttcgcctaac aacgccttta tagttgtcac
    34561 tggagatttt accaccgcag aaatcctgcc caagattaaa gctgcttacg gcaacatccc
    34621 gcgcggcgct gaacctccta aggttcacac tctggagcca ccgcaactag gcgaacgcag
    34681 ggccattttg aaaaaggaag cggagctgcc gatagtcact gcgaactatc acgcgcccaa
    34741 tttaaaaagc ccggataatt acgctctgga cctgctcacg gtggtcctcg ccggcggccg
    34801 cagctccagg ctgcatcagg acctggttta ccacaagcgc ttggtgcggg gggttgatgc
    34861 cgactacagc ggagcttcca tcgatccgac ggttttgtcg gtgacagcgc aactattacc
    34921 cggtaaagat cccgcaccga tcgagcggga gatcgacgga ttgattgaca aggtcaaagc
    34981 cgagctgatc agcgaccgag agctggagaa ggccaagaat caggttgaag cggcgttcat
    35041 tttcagtcgc gattcgattt tcggccaagc gatgaaaatt gggttctatg aggtggccgg
    35101 agattggcgg ctgatggacc gctatctcga aggcattaga aaagtgaacc gggaagatat
    35161 tcgtagagtc gctagacagt atctcgatcg tgatcgccgc acggtgggta ccctgattcc
    35221 cgtcaaaccc aagacgccat gaagaagatg ttttttctag tgctcgttgt cggctcactg
    35281 cttgttgcca caggcttcgg ccgaacggcg gtgacgccgc aacggtcggt gttgaataat
    35341 ggcatggtct tgctgacttc ggagcagaga accttgccga tggtctcgat agaaatgcta
    35401 atccacgctg gcgcgcgtta tgacgcgcct aagcaggatg ggcttgccaa tctcaccgcc
    35461 gagttgatca ccgagggcac caagcggcac gctgctctgc aaatcagtga aagtttggat
    35521 tttcttggcg caaccctatc ggcgcgcggc ggcgaggacc tcgcgagtgt tagcatgacg
    35581 gttctcaaaa aagatcttcc tgccggtttg gaactgctgg ccgaagtgtt gaccagtgcg
    35641 gtgttcccgc aagaggaaat tgagcggcaa aagcaagcgg tggtagcatc gattagagcg
    35701 aaacaagagc aacccgggga aattgccgag atgcgctttg ccggggcgat atttccgcag
    35761 acgcgctatg gccggccggt agagggaacc gagacatcgg tgaaggcaat cccgcaggca
    35821 agcttacgag agttttatca acggtattac cgtccgaatc gctcgatcat ggcaattgtc
    35881 ggcgatattt cacacgagga aatgatcgag gcgctcactc aggccttccg ctcgtggaac
    35941 aaaggagagc cgccggccaa accgttggtg ctaggtgctg cgccggaaac tggaacgata
    36001 cgagtgaata aggatctcac tcaagcgaat ctagttctcg gtcaacgcgg tgtcggccgg
    36061 gaaaaccccg actactatgc gcttcaagtg atgaactaca ttctaggcgg cggcgggttt
    36121 tcctcacggg caatggactc catccgcaat cagcgcggtc tggcctattc ggtttatagc
    36181 tatttcgacg ccgataaagg ccgcggtact ttccaattcg tcatgcagac aaagaatgag
    36241 accgctttgc aggcaattgc aatcgcaaaa gaggaaatcc gtcgcatccg ggaggcgccg
    36301 gttagcgagg ccgagcttaa cgatgctagg gattatctca ctggcagctt cccgctgcgc
    36361 tttgacacca tgagcaaggt cgcgaatttc ttggcccagg cggagcactt cgagcttggc
    36421 ctggattata ttcagcgcta tccggatctg atcggtcgaa ttactatcga cgacgttaaa
    36481 cgggtcgcac agaaatacct cgacccggaa aagatggtta ccgtcattgt cggaaaccaa
    36541 acgatcatcg ctgataaatg aattccggtg aaatcaagct cagatgcttg acggcggcta
    36601 aatgccgggg catatttgtg cggtcggcgc ccgactaaac tgaactcgat catcactgtc
    36661 cagcccggaa agcggcctgt ccatgcaaaa agttttaatc atcgaagacc acgccgatat
    36721 gcgtgaactg ctaagctggc aactcgaatt gatggggttc atgccgattt tagcgaagca
    36781 cggcaaagag ggtatagaaa aggctcttgc ggaaaagcct gagttgattt tgatggatat
    36841 catgatgccc ggaatggacg ggtgggagac gtgcacgcag atcaagtgcg acgccgtcac
    36901 ggccgacatc cccgtgattc tgctgaccgg cgccaacgat cgcgatctca cccagcacgc
    36961 gaacgcggtc ggcgcatcgg cgctcctgca gaaaccgtgt c
//
LOCUS       DASUVS010000278        11920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_551068, whole genome shotgun sequence.
ACCESSION   DASUVS010000278 DASUVS010000000
VERSION     DASUVS010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11920
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551068"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VF182_15385"
     CDS             complement(<1..616)
                     /locus_tag="VF182_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX6438519.1"
                     /translation="MAEELRIAKGSKEHSLLPQMANRHGLIAGATGTGKTVTLRVIAE
                     NFSRIGVPVFMADVKSDLSGIARAGQENPKIRERLDKLGLKGFDFVPYPTVFWDLYGE
                     QGHPVRTTISDMGPLLLSRLLSLNETQSGVLNLVFKVADDNGMLLLDLKDLRSMLQFI
                     GDNAAKFKTKYGNVSTASIGAIQRGLLALQEQGGEKFFGEPALDL"
     gene            848..1126
                     /locus_tag="VF182_15390"
     CDS             848..1126
                     /locus_tag="VF182_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438520.1"
                     /translation="MKRFLSVLVASMFLASAAYAQTGKSDTKKSSGSMQSESVKTDGK
                     EKSDSVKAEGKAKGKSKAESTSETDTKSTDGKTTSETKPAGKSKAATK"
     gene            1396..2124
                     /locus_tag="VF182_15395"
     CDS             1396..2124
                     /locus_tag="VF182_15395"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEX6438521.1"
                     /translation="MTQNGHPEAVIIHSSDLHLGTDDSFSQRDSLAVLPKVLNTAIET
                     QADIVVLAGDTFDNHRQPGELLERAAQILRDYGNPIVILPGNHDPLTPDSVYRRGGLA
                     QIPNVHILGLNVDQSVMFPQYELEIWGRAHVDYTDMSPLADPRPRSTRWQLAAAHGHY
                     VDEQRDPNRLIGSWLIHREELLATGADYVALGHWNQATPVGSGEITAYYSGSPEYAGT
                     VNVIRLTSRGAVEVSQAPLNGSFR"
     gene            2642..3196
                     /locus_tag="VF182_15400"
     CDS             2642..3196
                     /locus_tag="VF182_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX6438522.1"
                     /translation="MNEIGSRKPRVSQLDLFLAFSRISLSSFGGAIFWARRELVERQR
                     WFSEREFVEVYTLGQLLPGPNVLNLTVIVGHRFAGWTGAAAGVAGFLGWPCLMVVGMG
                     VLYQHYGALPFVQQALTGMSSVAAGLLLATVIKLATVLPRHWRPWLFGILAFVAVGIM
                     RWPLLWVMGALAPWAVLAAWKEPE"
     gene            3196..3723
                     /locus_tag="VF182_15405"
     CDS             3196..3723
                     /locus_tag="VF182_15405"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX6438523.1"
                     /translation="MDRLDLAGLFVHFLILWFIAIGGPSTILPDIHRYLVEVHHLLTN
                     AEFVEIYTLAQVAPGPNAMYVTLIGWHLAGWKGVAATTIPLVVPAATLTLLVGHLNER
                     YPNARGARAVRRGLMPITIGLVFASATILMRAVNHDWRGYLITLLTVAVVLRKPWNPL
                     WLFGAGALAGIAGFV"
     gene            4119..5309
                     /locus_tag="VF182_15410"
     CDS             4119..5309
                     /locus_tag="VF182_15410"
                     /inference="COORDINATES: protein motif:HMM:NF014510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HEX6438524.1"
                     /translation="MIDNPFWTVTLPEMLDVVFVAILLYTAIVWAQQTRAAFVVRGIL
                     ILGSIYIIAKYLDLQMTAWIFQGFFAIFLIMIVVIFQEELRQLFERIAVWSLARKRTP
                     TLGSSTSDVLVRTVADLAKEHVGALIVIRGNDPLERHITGGIPLEGKLSAPLLKSIFD
                     PHSPGHDGAVMVDQDNISRFAAHLPLSKDLRQLANVGTRHSAALGLAELTDALCIVVS
                     EERGTISVARDGKLREVQNIQELGAVLESFLQTKFPSSTQNNNFGLLFFRENWIAKAI
                     AIALAIGFWYIFVPGSKTVDVSYTVPVIVENLPPDLQVEDIEPPVVNATFRGPRRAFY
                     LFNSSKIKVAIDVSMAELGRRTFTISEKNIQYPKELTLQDVSPSTLRLSVRKVPIAND
                     TNRG"
     gene            5465..6097
                     /locus_tag="VF182_15415"
     CDS             5465..6097
                     /locus_tag="VF182_15415"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEX6438525.1"
                     /translation="MIDFEGRELPVELSEIVAPSRSVLLVWDMQNDQAGGAFNKADLI
                     RKSPPVIAAAAHRQIKTVYTRQTPFLWKDEAPTWIRRGMKDQKVNDPSQLKPRRLHGS
                     FGWQFMEPFKPAENDVIIDKRRPTMFIGNEFETILNNRGASTVVMIGCTTDGGVEATV
                     RDGHNRGYFMVVVRDCVGTYTEEGHNAALKRIERFGDLVDSSELIDLWRK"
     gene            6105..6503
                     /locus_tag="VF182_15420"
     CDS             6105..6503
                     /locus_tag="VF182_15420"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX6438526.1"
                     /translation="MAIDIREIMERFHGGAKEEAFFELLEMPGDVLPALTAAFRTERD
                     PALRAFLVTVGWERRDPMAIHLLSEALDERDEEIWQAALDGLVTFASPEALAILIAAR
                     TRELADESATKRFQSCVSEAIEYIRQTIPI"
     gene            6619..7590
                     /locus_tag="VF182_15425"
     CDS             6619..7590
                     /locus_tag="VF182_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438527.1"
                     /translation="MTESNGAKKALHVATLSGWYRFEQDGREWKQTKRDLSYWSLTCM
                     SVDPENPQKIYAGTEHSGLFYTVDAGASWHRAQPNVPKMMLFAILALNGRVMVGTIPS
                     AVFRSRNGGWEELEGVRINSAGANFPPSPELQSRTRYLTFDPASPNRIYAGIEVGGML
                     VSDDGGENWRPANEGLTDMDIHEVLASRNSAGLVVAACGEGAFRSFDRAAHWQEITPQ
                     SHDYGTSVAEDKNGVVYLGSAKGRPNGWIRKEGAMAAIFRSRDKGSTWEKIIDNLKGG
                     VMHMCSLPDGKGMIAGTSDGSLLVVDDSGAREIATGLPFVTSVELAA"
     gene            7682..8326
                     /gene="maiA"
                     /locus_tag="VF182_15430"
     CDS             7682..8326
                     /gene="maiA"
                     /locus_tag="VF182_15430"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEX6438528.1"
                     /translation="MKLYTFFRSSASYRVRIALNLKGLDYEQAPIHLRRGGGEQLKPA
                     YRVINPQALVPALEDDGRILSQSLAIIEYLEERYPKPPLLPSDAADRALVRSMALLIA
                     CEVHPIQNLRVLNHLKSDYQQSDDDTIRWARHWIDLGFSALEEMVNTGSSQGEFCFGK
                     TPTMADLCLVPQLANARRFGVDLASRPKLLAIEAACMSLPAFAKAAPEQQPDAE"
     gene            8452..9219
                     /locus_tag="VF182_15435"
     CDS             8452..9219
                     /locus_tag="VF182_15435"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX6438529.1"
                     /translation="MFVLKQFLKALVLPPTPWLLMLLAVLIFWQRRWARKLLLLTIVL
                     ITASHSGLVGYGLRYPLESRYAPLLDPSALSYDAIVVLTGGMIPPEGLIPFSTIDEHM
                     FRRLDEAWRLYRIQPRPIILSGGHVNPFTPDKDENRIGREYLIRWGVSKHHVIGEGKS
                     RDTFESAIEVQKLLKRRGWKRYLLVTSALHMPRSMMAFKAKAPEPLPAPGDYSLGKLR
                     LTPLDFFPGEGAARGIYATLHEYIGIANYYWRIQFDP"
     gene            9483..11129
                     /locus_tag="VF182_15440"
     CDS             9483..11129
                     /locus_tag="VF182_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX6438530.1"
                     /translation="MRVVDAISQILKREGVDFLACYPTNTLIEAAAAAGIKPLICRQE
                     RVGVGIADGYTRISNGRRIGVFSMQAGPGAENAYSGITTAYSDSVPVLLLPVGHARST
                     SQVHRFFRSTQSYATVTKWTEEIGLACHTSDVMRRAFSLLKMGRPGPVLVEIPGDVAT
                     EEINESQLEFAPVKRTVSGANPQDIAEAARTLVQAQAPIIVAGQGVLYAEASDELLEF
                     AELLQAPVTTPLEGKGAFPETHRLSLGTGAGVMPPTVHEFLRTADVVFAVGASLTRHS
                     IVAAPIPAGKTIIHATNDEHDLNKHYIADIPILGDAKPVLRQFIEAVKDAQGANRPKK
                     ADLVAAKIKEIREPWLKQWMPKLTSKEVPINPYRVFWEFMRAFDPKDSIVTHDAGSPR
                     NQLVPFYQAPTPRSYIGWGKSHALGTGLGLIMGAKVAAPEKFCVNFMGDAAFGMTGLD
                     FETAVRCNIPILTVVLNNNFMAAETRHMATSHKLFGTQNVGGNYAEIGRALGGWSEKV
                     EDPDEIPAALQRARKATQDGKAALLEFITSREHSYSRFGE"
     gene            11577..>11920
                     /locus_tag="VF182_15445"
     CDS             11577..>11920
                     /locus_tag="VF182_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438531.1"
                     /translation="MESERLFLWIGFNLFVLGMLALDLGVFHRKAHTISIKEASIWSV
                     VWVCLAMIFNVGIYYAWGSEAALEFLTGYVIEKSLSVDNLFVFLMIFQYFNTPSQYQH
                     RVLFWGILGALV"
BASE COUNT         2694 a         3285 c         3220 g         2721 t
ORIGIN      
        1 cgagatcgag cgctggctcg ccaaaaaatt tctcgccgcc ctgctcttgc aacgccagca
       61 ggcctcgctg tatggcgccg atacttgccg tcgaaacatt gccgtacttg gtcttaaatt
      121 tagctgcatt gtcgccgatg aattgcaaca ttgagcggag gtccttgagg tcgagcagca
      181 gcatgccatt gtcatcggcg actttaaaaa ccaggttgag tacgccgctt tgggtttcgt
      241 tcaaactaag tagccggctg agcagcaacg gccccatatc cgaaattgtc gtgcgcactg
      301 gatgcccctg ttcgccgtac agatcccaaa agaccgttgg ataaggaaca aagtcaaatc
      361 ctttcaggcc gagtttatcg agtcgctcgc gaattttcgg attctcttgt ccggccctgg
      421 cgattccgga caaatcgctc tttacgtctg ccatgaacac cggcacccct atacggctaa
      481 aattttcggc gatgactcgt agcgttaccg tctttcccgt gcccgtcgca ccggcgatta
      541 aaccgtggcg attggccatc tgcggcagta acgagtgctc tttgctccct ttagcaatgc
      601 gcagctcctc agccatgaga tgcacctcct tcgttgaggt aaaaaatcat aaattgccta
      661 tctaacgaac tattggtcaa aattggtcac ttgatgtata gaccagcggt gacgtgaatt
      721 aaagcacagc gttacacgtc taaggcatca agaacgaacg agatgagttc gagctgagag
      781 gagggcggcg cgcttgatca cggcgttctt aactaaacaa cgataaatca tacgggagga
      841 tcacaccatg aaacgtttcc ttagcgtgtt agttgcttca atgtttttag cttcagcagc
      901 ttatgcccag acaggtaaga gcgacaccaa gaagagctcc ggtagtatgc aatcggagtc
      961 ggtaaagacg gatggtaaag aaaagtctga ttccgtaaag gccgaaggaa aagctaaagg
     1021 caaatcgaag gccgaatcca caagcgaaac cgacactaaa tcgaccgatg gcaaaactac
     1081 aagtgagacc aagcccgccg gcaaatccaa agccgcaacc aagtaagctt tgatttcaag
     1141 ttgccaaccg ggtcttcctc cgcacccggt tggcacttaa aacccacagt tccgcacatc
     1201 cgatcagtcg gtaagcaaat ccgctaagct gtttcgctcc accctcaggc cagccgccag
     1261 catcacagaa tccttcatct tgtttgattc acacgtatgg tggaggcgcc ggtagctgcc
     1321 cttgcttgca atttccccac tgtgcccata agaataactg attggtttat ttgcgtcagc
     1381 gaaagtagga tctctatgac ccaaaacggg cacccggaag cggttatcat ccacagctct
     1441 gatttgcacc ttggcaccga tgacagcttc agccagcgcg attccctagc ggttcttccc
     1501 aaagttctca acacggccat cgaaacgcag gcagacatcg tcgtactggc tggagatact
     1561 ttcgacaatc accgccaacc tggcgaactt ctcgagcgcg ccgcgcaaat tttgcgtgat
     1621 tacggcaatc ccatcgttat cttgcccggc aatcacgacc cgctgacgcc ggattcagtc
     1681 taccgtcgtg gcgggcttgc gcagatcccc aacgttcaca tactgggtct taacgttgat
     1741 caatccgtga tgtttcccca atacgaactg gagatttggg gtcgagcgca cgtcgactac
     1801 acggatatgt cgccacttgc cgatccccgg ccgcgttcga cgcggtggca gcttgccgct
     1861 gcccatggac attacgtcga tgaacaaaga gatcccaatc ggctgatcgg ctcgtggctg
     1921 attcaccgcg aagaattgct cgctaccggc gcggattacg tagcgctggg ccactggaac
     1981 caggcaactc ccgtcggctc cggcgagatt accgcctact actccggctc gcctgagtac
     2041 gccggcactg tgaatgtgat ccggctcaca tccaggggcg cggtcgaagt cagccaggca
     2101 ccgctgaacg gaagttttcg ctaaaaaagt attttccaag cgcgagactg tgcacccgct
     2161 cgaacgctag caacactcat ccatacaacg ccaatccctt cctatttttt tcgcaacgag
     2221 cgctgcgata gctaccgatc tccgctgctt tagctcgcct tagccgaacc atctgaaccg
     2281 gaatagtagg aaaccatgaa accgtctttc aaacggctga aggcttagac aaggagcaag
     2341 cgagttctct ttagcatccg tgtgaattaa gaattttttc ctacaatgct ggtaaatcag
     2401 taacattgaa tggtaacttc tcacctgaga aattggcacg atatgcgaga agaagcaggc
     2461 ttcgactcta gatcgagtta gctccgcttt tcctacagag tgctcgagat taaaaccgaa
     2521 ggctgctcga tcaattcgat tccgaacagc tgtgctcaaa taaactttcc cactttcgaa
     2581 tctcggcgtt ctcgtgccga gcaggtcgcc gttgattaca acaagttgca cgcgaaacaa
     2641 catgaatgaa ataggatcac gcaagccgcg cgtcagccag ctcgatttgt ttctggcttt
     2701 ttcccgaatc agcttgtcga gcttcggcgg cgcaattttt tgggcaagac gcgagctggt
     2761 cgagcggcag cgctggttca gcgaacgaga gtttgtcgag gtctacactc tcggacaact
     2821 cttacctggg ccgaacgtgc tcaatcttac ggtgatagtc ggtcatcgtt tcgccggctg
     2881 gacgggtgcc gcggcgggcg tggccggctt tttgggttgg ccttgcctca tggttgtcgg
     2941 catgggcgtg ctctaccagc attacggagc gttgcctttt gtgcagcagg cactcaccgg
     3001 tatgtcaagc gtggcggcag ggctgctgct ggcgaccgtg atcaaattgg caaccgtatt
     3061 gccgcgacac tggcggcctt ggctgttcgg gatactcgca ttcgttgcgg tcggcatcat
     3121 gcgctggccg ctcttgtggg tcatgggtgc tctcgcgccc tgggctgtgt tggcggcctg
     3181 gaaagagccg gagtaatgga tcggcttgat ctggccggat tgtttgttca ttttctaatc
     3241 ctctggttca ttgccattgg tggtccatcg acgatcctgc cggacattca ccgctacttg
     3301 gtcgaagtgc accatctgct gaccaatgcg gaatttgttg agatctatac cttagcgcag
     3361 gtcgcacccg gacccaacgc catgtacgtt actctgatcg gctggcactt ggctgggtgg
     3421 aagggagtag ccgccacgac gatcccactg gtggtgcccg ctgccactct caccttgctg
     3481 gtcggccatc tcaatgagcg ctaccccaat gcccggggtg cccgggccgt tcgccggggt
     3541 ttgatgccga ttacgatcgg acttgtgttc gcgagcgcaa cgattttgat gcgcgcggtg
     3601 aaccacgact ggcgcggtta cttgatcacg ctcctcaccg tcgccgttgt cctgcgcaag
     3661 ccgtggaatc cgctttggct gttcggcgcc ggtgcgctgg ccggtatcgc ggggtttgtc
     3721 tgagcattgc ggcgcacgaa aatcgcgctg attcagcgtt tgctgcgcgg agaaaaggca
     3781 gccccgaact gtgattacac acgtaatttc aattatccat aatggatcgc cgctcgctat
     3841 tgagagctca agctgaactg cggtgcccgg ccaacactcg cgtacgtcgc aaatcgaagt
     3901 tcatctcggc tcctggatcg ggcagcgact cactcgcaca cttgggcttc ccatctctgc
     3961 tgtgtttgaa ttttcaaaat ggtataactt cgacgcctgg cgtgctattt tcgaaacaaa
     4021 gcgcagagaa tgcgctacgg cccacaagca tcatcgatca tgcgaacggt ttacaacggt
     4081 gatgccggca gcaaaatcta ccgggactct gcataaaaat gatcgataac ccattctgga
     4141 ccgtcacctt gccggagatg ctcgacgtcg tctttgtcgc tattctcctc tataccgcca
     4201 ttgtctgggc gcaacagacc cgcgccgctt ttgtagttcg aggaatttta attctcggtt
     4261 ccatctatat cattgccaag tacctcgatc tgcagatgac cgcatggatc ttccagggtt
     4321 tcttcgccat ttttttgatc atgatcgtgg tcatctttca ggaggaactg cggcaattat
     4381 tcgagcgcat cgcggtctgg agcctggcgc ggaaaagaac ccctactttg ggttcgagca
     4441 cgtcagacgt cctggtccgc acagtagccg atctcgccaa ggaacacgtc ggtgcgttga
     4501 ttgttatccg cggcaacgat ccgctcgagc ggcatatcac cggcggaata cctctggagg
     4561 gcaaactaag tgcgccgctg ctgaaaagca ttttcgatcc ccactcaccc gggcatgacg
     4621 gtgcagttat ggtcgatcaa gataacatct cgagattcgc ggctcacctg ccgctttcga
     4681 aggacctcag gcaacttgcc aatgtcggca cccggcactc ggcggccttg gggttggccg
     4741 aactgaccga cgctctgtgt atcgtagtgt ccgaagaacg cggcacaatc tcggttgcca
     4801 gagacggcaa gcttcgcgag gtgcagaaca tacaagagct tggcgcggtt ctcgaaagct
     4861 ttttgcaaac gaagtttcca tcgtcgacgc aaaacaacaa tttcggtttg cttttttttc
     4921 gcgagaactg gattgccaaa gcgattgcca tcgcgctggc aattgggttc tggtacatct
     4981 tcgtgcccgg atcaaagaca gtcgacgtca gctacacggt tccggtgatc gtcgagaatc
     5041 ttccccccga tctgcaagtc gaagacatag agccgccggt ggtaaacgcc accttccgcg
     5101 ggccgaggcg ggctttctat ctgtttaatt cgagcaagat caaagtcgca atcgacgttt
     5161 ccatggcgga gctggggcga cgaactttca cgatttcgga gaaaaacatc caatatccga
     5221 aagaactcac actgcaggac gtcagcccgt caactctgag gctgtcagtg cgaaaagtcc
     5281 ctatcgccaa cgacaccaac cgcggctagg gaatgagctg ggctggtgat catgcgattt
     5341 tagatggctc ggcacagaga ccaacaacac gacaacaccg gcgagggcag atcccggacg
     5401 gtgaatggct ttttcatggg tatcttagcg gtccagcgag aacccggaag gaagcagcac
     5461 gattatgatc gacttcgagg gaagagaact acccgttgaa ctgagcgaaa tagttgcacc
     5521 gagccgttca gtactgctgg tttgggacat gcaaaatgat caggccggcg gtgccttcaa
     5581 caaagccgat ttgatacgca agagcccacc ggtaatcgcc gcagctgctc accggcagat
     5641 caaaactgtc tacacgcgac aaacgccttt cttgtggaag gacgaagcgc cgacgtggat
     5701 ccgcagaggc atgaaagatc agaaggtcaa tgatccaagc caactgaaac cgcgccggct
     5761 ccacggcagc ttcggctggc aatttatgga accgtttaaa ccggctgaga acgacgtgat
     5821 catcgataag cgccgaccga cgatgtttat cggcaatgag ttcgaaacga tcctcaacaa
     5881 tcgcggcgcc tcgactgtgg tgatgatcgg ctgtaccacg gacggtggcg tcgaggcgac
     5941 agttcgcgat ggtcacaatc gcggttattt catggtcgtt gtgcgcgatt gcgtcggcac
     6001 ctataccgaa gaaggtcata atgccgcgct taaacgcatc gaacgctttg gcgatctggt
     6061 ggattcttcc gaattgattg atctctggcg gaaatgagtc cgcggtggca atcgacatca
     6121 gagaaattat ggaaagattt cacggcggcg ccaaagaaga agcatttttc gaactgctgg
     6181 aaatgcccgg tgatgtgctt ccggcgttaa cggcggcttt ccgcaccgaa cgagatccgg
     6241 cgctgcgggc ttttctcgtg acggttggct gggagcggcg cgatccgatg gcaattcatc
     6301 tcctgagtga agcgctggac gaaagggacg aagaaatttg gcaagcagca ctcgatggtt
     6361 tagtgacatt tgcctcacct gaggcactgg cgattctcat agcggcgaga acccgcgagt
     6421 tggccgacga atccgcgacc aaacgttttc agtcctgcgt cagcgaggcg atcgaataca
     6481 tccgccaaac catacccatc tgacatccgc ttgccagaac catattagtc atatgcaggc
     6541 aaccacgcgg ttgcgatttg tcccctgaac gagttagaag tgtctcactt cggtgcggca
     6601 caataaaagg agataaccat gacggaatcc aatggcgcga aaaaggcatt gcatgtcgcg
     6661 acactctctg gttggtatcg tttcgaacag gacggtcgag agtggaaaca aaccaaacgc
     6721 gatctgtcgt attggagctt gacctgtatg tcggttgacc cggagaatcc gcagaagatt
     6781 tacgcaggca ccgagcattc cggtctgttc tataccgtcg atgcgggagc gagttggcat
     6841 cgcgcgcagc ctaacgtccc aaagatgatg ttgttcgcca tactcgcatt aaatggccga
     6901 gttatggtgg gaacgattcc atcagccgtt ttccgcagca gaaatggcgg ctgggaggag
     6961 ttggaagggg tacggatcaa cagcgcgggg gcgaattttc cgcccagtcc ggaactgcaa
     7021 tcacggacgc gttatttgac gttcgatccc gcttctccca atcgtatcta tgccggaatc
     7081 gaagtcggcg gaatgctcgt tagcgatgac ggcggtgaga actggcggcc cgccaacgaa
     7141 ggactgaccg acatggacat tcatgaagta ctggcgtcga gaaatagtgc aggattggtg
     7201 gttgcggctt gcggcgaggg cgcgtttcgc agtttcgatc gtgctgcgca ttggcaagag
     7261 attactcccc agagccatga ctacggcaca tccgttgctg aagacaaaaa tggagttgtc
     7321 tatcttggta gcgccaaggg gcgcccgaat ggatggattc gtaaagaagg cgcgatggcc
     7381 gctatttttc gcagccgcga taaaggatcc acctgggaga aaatcatcga caatctaaaa
     7441 ggcggcgtca tgcatatgtg ttcactgccc gacggcaaag gcatgatcgc aggcacctcc
     7501 gacgggagcc ttctggtggt cgatgattct ggcgcgcgcg agattgccac cggtctgccg
     7561 ttcgtcacat cggtcgagct agcagcttag aaccgtcgca gacaggctgc tgtgatacct
     7621 taagctccag gatcagcaat tatcgagctg gatactcctg cagctcgtga aggtctgaaa
     7681 gatgaaactc tatacctttt ttcgcagttc ggcatcgtac cgcgtacgca tcgcgcttaa
     7741 tttaaaaggt ttggattatg agcaggcacc gatacacttg cgccgcggcg gcggcgagca
     7801 gttaaaaccg gcgtaccggg tgatcaatcc acaggctctg gttccggcac tcgaagatga
     7861 cgggcgaatt ctttcacagt cattagcaat tatcgagtat ctggaagagc gctatccaaa
     7921 accgcctttg ctccccagcg atgccgccga ccgcgcgctc gtgcgcagca tggcgctgct
     7981 gatcgcctgt gaggtgcacc cgattcagaa tcttcgtgtg ctcaatcatc taaaaagcga
     8041 ttaccaacaa tcggatgacg acacgatccg ttgggcgcgt cattggatcg atctcggatt
     8101 ttcagcgcta gaagagatgg tgaacacggg ctcctcacag ggagaatttt gcttcggcaa
     8161 aacaccgaca atggccgatc tctgcctcgt gccgcaactc gccaatgcgc gccgcttcgg
     8221 tgtcgatctg gcatcgcgcc caaagctgct cgcgattgaa gcggcctgca tgtcactacc
     8281 tgcgttcgcc aaggccgcgc cagagcagca accggacgcg gagtagttta acgcttccca
     8341 atgatctgcc gattagttga atctttaatt gtcatggccg agcctcgccc ggacgattcg
     8401 acatcccttt tcaatttaag cctttcaatc tcagccttct gatcaccatc gatgttcgta
     8461 cttaagcagt ttttaaaggc gctcgttctg ccgccaactc catggttgct gatgctgctc
     8521 gcggtgctga tcttttggca acggcgctgg gcgcgcaagt tattgctgct cactatcgtg
     8581 ctgatcaccg catcgcacag cggcttggtt ggctatggcc tgcgctatcc tctcgaatcg
     8641 cgctacgctc cgctgctcga tccgagcgcg ctctcttacg acgccatcgt ggtcctcacc
     8701 gggggtatga tcccgccgga agggttgatt cctttctcga ctatcgacga gcatatgttc
     8761 cgacgtctgg atgaagcctg gcgtctttat cggatccaac cccggccgat tatcctatcg
     8821 ggcggccacg ttaatccgtt cacacctgac aaggatgaga atcgcatcgg ccgcgagtat
     8881 ctgatccgct ggggcgtgtc caaacaccat gttatcggcg agggcaaatc ccgtgatacg
     8941 ttcgagagcg caatcgaagt gcaaaagctt ctcaaacgcc gcggctggaa gcgctacttg
     9001 ttggtgacct cggcgttgca catgccgcgc agcatgatgg cattcaaagc caaagcgccg
     9061 gaaccgcttc cggcgcccgg cgattattcg ctgggcaagc tacggctcac accgttggat
     9121 ttttttcctg gcgaaggcgc cgctcgcggc atctacgcca ccctgcacga gtacatcggc
     9181 atcgccaact attactggcg gattcagttt gatccgtaac agcaccatcc gtacgttgag
     9241 caaatctcac gcgcccggat gcttcgttgc ctatatccgg acgacatcaa gtaaaccgcc
     9301 ggttgaaaaa cctttccgga gtgatagcgt ccatgggctg gcgggctatc gccgctgtgt
     9361 ggcgcagtcg ggtcagtccc cattttgatt ctgatccgtc aaaaaaccaa agcaggatct
     9421 tgaagaactg cttgttgcat taatcgaaag atcaatccgg tccgagcgca acgaggtgaa
     9481 cgatgcgagt tgtcgatgcc atcagccaga tcctcaaacg tgaaggtgtg gattttcttg
     9541 cttgctatcc aaccaatacc ttgatcgaag ctgcggcggc ggccggaatc aagccgttga
     9601 tctgtcgtca agagcgagtc ggggtcggca tcgccgacgg ttacacccgg atctccaatg
     9661 gccggcgcat tggcgtattc tcgatgcagg caggtcccgg cgcggaaaac gcatattccg
     9721 gcatcactac cgcctactcc gattcggttc cggttctgct tttaccagtc ggccacgccc
     9781 ggtccacgtc ccaggttcat cgcttcttta gatcaacgca atcctacgcg acggttacca
     9841 agtggactga agaaattggc ttggcttgtc ataccagcga cgtcatgcga cgggccttca
     9901 gccttttgaa aatgggccgc cccggacccg tgctcgtcga gattcctgga gatgtagcca
     9961 cggaagagat taacgaaagc caattagagt ttgcgccggt caaacgcacc gtaagcggag
    10021 ccaatcccca agatattgcc gaagccgcgc ggaccttggt ccaggcgcag gcgccaatta
    10081 tcgtcgccgg acaaggcgtg ctctatgccg aggcatcgga tgagttatta gagtttgcag
    10141 aactcctgca agcgcccgtg actacgccgc ttgaaggaaa gggcgcattc ccggaaactc
    10201 accgattatc gctcggcacc ggcgccggcg tcatgccccc cacagtgcac gagtttctgc
    10261 ggacagccga tgtcgtgttc gctgttggcg caagtcttac ccggcatagc attgtcgccg
    10321 cgcctattcc cgccggcaaa accatcattc acgcgactaa cgacgagcac gatctcaata
    10381 agcactatat cgccgacatt ccaatcctcg gtgatgccaa gccggtgctc cggcaattta
    10441 ttgaagctgt aaaagatgct caaggagcaa accgacccaa aaaggctgat ttggtagcgg
    10501 cgaaaatcaa ggaaatacga gagccctggc tgaagcagtg gatgcccaag ctcacttcca
    10561 aagaagtgcc gatcaatccc tatcgagttt tctgggaatt catgcgcgcc ttcgatccga
    10621 aagactcgat tgtcactcat gatgccggca gcccccgcaa ccaactggtt cctttctatc
    10681 aagcccccac gccgcgcagt tacatcggtt ggggaaaatc ccatgctctg ggcactggcc
    10741 ttgggctgat catgggcgcc aaagtggcag cgccggaaaa attttgcgtc aactttatgg
    10801 gagatgccgc cttcggaatg accgggctgg atttcgagac cgcggtccga tgcaatattc
    10861 caatcttgac tgtggtgctg aacaataatt ttatggccgc tgaaacgcgt cacatggcaa
    10921 catcgcacaa gctcttcggt actcaaaacg tcggcggcaa ctatgccgag attggccggg
    10981 cgctcggcgg ttggagcgaa aaagtcgagg atccggatga aatcccggcg gctttgcagc
    11041 gcgccaggaa ggctactcaa gacggcaagg cggcgctctt ggaattcatc acgagccgag
    11101 aacacagtta ttcccggttc ggcgagtagc ggattgactt gaacttgcct aattgttacg
    11161 cgtgtgtgtt gatgctgccc gcggcgcgtt cgataatctt gttactgttc ctgactctcg
    11221 gcttctcagg gatagatcct ttgatgcttc aaacgccgct aggggagtcg ggcgacgatc
    11281 ctatttcttc ggaaacacgg caagaacaac ttatgcgggt tgccgaagcc cggaaagttc
    11341 gaacccccgg ggaaaagtgg agtcaatctt tcatctcgcg actagatcac cgcttactgg
    11401 ggaagacagc ccaagcgtca ttcacatcac tttcaccccc gcacgccagc ccaggtaatt
    11461 tgcatgggct actgcaagtc tttcgcatct agactttcct tctccggtcg ggttgccggt
    11521 caacacggcg cagagcggca cgcgccgaat attcaggaga acggaaagtg acggatatgg
    11581 aatcggaacg attatttctt tggattggct ttaacctttt tgtcttgggg atgctcgccc
    11641 tggatttggg tgtttttcac cgcaaggctc acacgatctc gatcaaagaa gcatcgattt
    11701 ggagcgtcgt gtgggtctgc ttggcgatga tcttcaatgt tggaatttat tatgcctggg
    11761 gatccgaagc agccctggag ttcctcaccg gctatgtcat cgaaaaatct ctgagcgtcg
    11821 acaacttgtt tgtgtttcta atgatctttc aatatttcaa tacgccgagc cagtaccagc
    11881 accgagtgct tttttggggc atcctcggcg cgttagtatt
//
LOCUS       DASUVS010000279        13265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1425590, whole genome shotgun sequence.
ACCESSION   DASUVS010000279 DASUVS010000000
VERSION     DASUVS010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13265
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425590"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VF182_15450"
     CDS             complement(<1..401)
                     /locus_tag="VF182_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX6438532.1"
                     /translation="MVEPNESLDQRLCTRCCIAGGGPAGMMLGFLLARAGVDVIVLEK
                     HADFLRDFRGDTIHPSTLEIMYELGLLDEFLKLPHQKVYQLSAQIGDTQLNLADFREL
                     PVRCPFIAFMPQWDFLNFLADSARRYPTFRL"
     gene            583..792
                     /locus_tag="VF182_15455"
     CDS             583..792
                     /locus_tag="VF182_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438533.1"
                     /translation="MRKLLVFQHVAHEILGTLNPLLKRAGFRIRYVNFGRHPDAQPSL
                     DGYHELVILGGRRVFTKIIASTPDN"
     gene            complement(1334..2749)
                     /locus_tag="VF182_15460"
     CDS             complement(1334..2749)
                     /locus_tag="VF182_15460"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6438534.1"
                     /translation="MRKRGYRTLSMIFGLTLLVVSLSGNSLAAEGSLPGNAPEQEAVS
                     LLSHYIQIDTTNPPGNEIKAAQFFKQIFDREGIEARIIESAPGRGNIYARLRGSGAKK
                     AVILLNHMDVVPADTKLWKEAPFSGTVKNGDIWGRGALDMKGPAIAELMTLLTLKRNK
                     VALLGDVIFLGTADEEAGGALGAEFLLEKHSELFADVGLVLNEGGGVRLGSDGKVQSY
                     SVSVAEKTPLWLRLIATGAAGHGSTPVKEAAVDKLVLALGRLLAYQTPIKVVPEVQKF
                     FADTAGEEPESHRGKYQDLRAALKDPAFAADFTRNTRNNASVRNTISVTRLSASDKVN
                     VIPAQASADIDIRLLPGEDPQAFIGEVRRVIADENIKIEVLLSFPAAVSPAHPEAIKA
                     IEELARTHDHGTPVVAPLVRGFTDCHFFRARGIPCLGFMPLRNIAAEGLVHGIDERIS
                     IDSLRVGVRALYELVHKLAGE"
     gene            complement(2796..3713)
                     /gene="miaA"
                     /locus_tag="VF182_15465"
     CDS             complement(2796..3713)
                     /gene="miaA"
                     /locus_tag="VF182_15465"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX6438535.1"
                     /translation="MKPKLAIVLGPTAVGKSDTVLDLALHFDAEIISADSQQVYRHMD
                     IGTAKPSKEQRQTVPHHLIDIVDPDEEFNAAMFRNLATEIAYKLADRDKRTIVCGGTG
                     LYIKALTQGLFAGPGGDPQIRQALEWEIEEYGMDWLYRRLERVDPESAARIHPHDRQR
                     ITRALEVCYATGRKISEWQSEHGFNDLLFDVLKIGLDRQRSDLYDRIDRRCEFMIGAG
                     LLDEVEKLVNMGYGLDLKAMQSIGYRHLGLFLRAQISLDHAVHLMKRDTRRFAKRQLT
                     WFHSDREIRWFDPETQRVNIEDAVREFYA"
     gene            complement(3765..5507)
                     /gene="mutL"
                     /locus_tag="VF182_15470"
     CDS             complement(3765..5507)
                     /gene="mutL"
                     /locus_tag="VF182_15470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX6438536.1"
                     /translation="MKIQVLSRETASRIAAGEVIERPASVIKELLENSIDAAASEITI
                     SVEKSGTSLIRVSDNGEGMSPEDLPLALERHATSKLRSDDDLFRISTLGFRGEALPSI
                     ASVSRFEITSRQAGHESAYRVRVDGGDRTSAHITAASIGTTVELKDLFYNVPARRKFL
                     KSLTTEFSHICDIVNRLALAYPEIHFRLEHNGKILIDFVGMRELRERLHQVLGREAAD
                     SLQPFEFRQGILSVTGYLSSAPTAFPNSRHLLTFVNRRYVRDKILTHAVLHGYETLLM
                     KGQYPAVVLLLDIPFDEVDVNVHPAKYEVRFRRQSDVHDAVARAIRQALRAEAQQPSK
                     RSFAPWPQYSSGVMEASLPYAISVSDPVESLVSETRREVFVLPNHSAQTRAGFFSSLN
                     VLGQILGCYLVCSSPRGLALLDQHAAHERVAFERLRQQMRANAVERQTLLLHQTVELS
                     AGESVMLEKQLDVLERYGFTLEPFGPRSYAIIAVPALLPEGDYRPVVRQMVAELAEID
                     QSEKLSQHLEERLATIACHSVIRANRQLEMHEMRALLRDLDDTEFATQCPHGRPVLLE
                     FSREELDRMFKRVS"
     gene            complement(5740..6027)
                     /locus_tag="VF182_15475"
     CDS             complement(5740..6027)
                     /locus_tag="VF182_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438537.1"
                     /translation="MAKRKRRKRSRGKTILLFVLAPLTVWALAFLVWLYWNEITRSLA
                     PADDHPESAPNRSRNLNKDERRTRSDETGAKEKISDTDRKKLDEIIRQENR"
     gene            complement(6128..7078)
                     /gene="glpX"
                     /locus_tag="VF182_15480"
     CDS             complement(6128..7078)
                     /gene="glpX"
                     /locus_tag="VF182_15480"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014244182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX6438538.1"
                     /translation="MDRNLALEAIRVTEAAALSCARLTGRGDEKAADQAAVDAMRRAF
                     DALPIDGTVVIGEGERDEAPMLFIGEKVGSGGPKVDIALDPLEGTTICATGAPNALAV
                     IAMADGGNLLHCPDTYMEKIAVGPAGKGVVDLNKTPTQNLQALAEAKRCNVEDLTVII
                     LSRPRHEALIKEVRQAGARIRLIGDGDVSAAIATTKPETGIDLLLGIGGAPEGVLAAA
                     ALRCVGGEFQGRLAPRNNEEIERAKKMGVSDIKKKFSIEELAAGDVMFAATGVTDGDY
                     LDGVHFFPGGATTQSVVMRSKTRTIRIINATHYFQHKPNY"
     gene            complement(7063..8283)
                     /locus_tag="VF182_15485"
     CDS             complement(7063..8283)
                     /locus_tag="VF182_15485"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HEX6438539.1"
                     /translation="MKFIILQGDGMADYPLEVLGGKTPLEAARTPNMDWLAARGVFGL
                     AHVIPKGFPPGSDVGNMSIMGYDPAVYHTGRSPLEAASMGVALGPNDIAFRCNLVTLT
                     GSESDRVMEDFTSGHISTQEAGMIIGDLAGALGADGIEFFPGVSYRHLMVWRDGKEKM
                     KTTPPHDITGQKISGFMPEGEGADRLLKLMEASHGILADHPVNKMRRAQGQRRATSIW
                     LWGQGRAPALPPLTKRFGIKGGVISAVDIIHGLGVYAGLERIDVPGITGFLDTNYRGK
                     GEYGLKSLENNDFVFIHVEATDEAGHMGDVEKKIQALEDFDEKVVGTVLTGMAHRKDW
                     RVLLMPDHPTAIALKTHVSDPVPFVLYSPQEARNNGSVGYNEKDAAKTGVVAKQAFRL
                     MEALIEGRQTWTEI"
     gene            complement(8423..9811)
                     /locus_tag="VF182_15490"
     CDS             complement(8423..9811)
                     /locus_tag="VF182_15490"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX6438540.1"
                     /translation="MTARRKIGVGLLGAGVVGEAIQDSLLAGFHGIVGQDLELELRRI
                     YTRNPKTKKWFKDRPELFTSQAIDVIDDPTVEIVIEALGFQQPGDLPIFRDYILRAFE
                     KGKSVVTSDKAVLARYGAEIWAAAQQSGQHLRFEACVGGGIPVIRSLNESFAVEEPEA
                     VFGIVNGTCNYILSQMEKTAKPYAEALKEAQQKGYAETNPTADVSGSDAEAKLMLLSS
                     VVFGLQLRPGTTWRKGIEDIHAVDFRYAGRTGASTIKHLAVAKRIGSSVQVFVSPVLM
                     PRDNLLAGIHGATNAICFNGKKSGGIRPHRDCDYVLVGPGAGGGPTAVAVLGDVCELA
                     RAKPGWFTGMPSLVQPGLLSLQPQDDIAGSFYVRFIVKDRPGIVGDIGQTFGAMGVNI
                     AEIWQLRHGDEELQDLARSYNLRADFKEILPFVMTLERATLRQIRAALDSVRDRDYIV
                     VDPVWFPIWAVS"
     gene            complement(9862..11040)
                     /gene="alaC"
                     /locus_tag="VF182_15495"
     CDS             complement(9862..11040)
                     /gene="alaC"
                     /locus_tag="VF182_15495"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HEX6438541.1"
                     /translation="MEFQRIKRLPPYVFSIIDGMKMQARHRGEDIVDFGMGNPDLPTP
                     PHVVAKLIEAASKPQNHRYSVSRGIYKLRVAISDWYQRRYQVAIDPDAEAIVTIGAKE
                     GLSHLAWATIDPGDVVLCPSPTYPIHQYAVVLAGGDLRCIPLITSEEFFGHLEEAIRQ
                     TWPKPKMLIISFPHNPTTEVVELDFFERIVQFAKEYELIVVHDLAYADLTFDGYRAPS
                     FLQVPGAKEVGVEFFSLSKSYNMPGWRVGFAVGNKDIIHALARIKSYLDYGIFQPVQI
                     AAIQALNGPQDCVEDIRAMYQKRRDCLIDGLTRAGWPIRKPKGTMFVWAEIPQAAKKM
                     GSIEFAKFILQEAKVAVSPGIGFGQYGDDHVRFALIENEHRTKQAVRNIKRALGKLLD
                     "
     gene            11401..12408
                     /locus_tag="VF182_15500"
     CDS             11401..12408
                     /locus_tag="VF182_15500"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6438542.1"
                     /translation="MTKTFPILVICSFTFLASSVCAQEPKLIEAAKKEGGKIVAYGSL
                     ESDTTEAVKQAFEKKTGLRMEYWRASATKVVDRATSEFRAQKPLFDVILVHDAEMRLL
                     LKNSVISRYNSPAFKDFTKESIDADLGPRYRDTIIGIVYNKSMIKPEDAPKSMEDLLQ
                     PKFRGKLVMPDPTQHTTTLQWVASLEKVMGKEKADRFVRDLAATKPLLVESLLPAAER
                     VSSGETPIAITYIKFSYLFGLKGAPLDYVRSDKMLGEGSYIMLASKPAHANAGKAFID
                     FFLDDESMKILAKNGEFVTRKGIHPPIPGAEKIKFIEMTHFDQNGFTEKKKEYQKIFL
                     Q"
     gene            complement(12696..>13265)
                     /locus_tag="VF182_15505"
     CDS             complement(12696..>13265)
                     /locus_tag="VF182_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6438543.1"
                     /translation="HSRGALPAARLAVDHPELVKALIIFDSNTLPAEDASLPYDFYTK
                     LEVGAPAVPDREFILREPIANSYSHDHITEDFVEEMLRIGRLPRTLEARKKMERFLDE
                     VFLPSMREKKYETLKLIQAGGLKAPTLIIWGINDQSSPMKLGIDLLHVISSVVDRTEF
                     HAFNHAGHYVFREQARHVNRLIIDFVQSL"
BASE COUNT         3056 a         3734 c         3480 g         2995 t
ORIGIN      
        1 aataatcgga atgtaggata acgacgcgcc gaatcggcaa ggaaattcaa gaagtcccac
       61 tgcggcataa aagcgatgaa cggacagcgc actggcaact ccctgaagtc agccaagttg
      121 agttgggtgt cgccgatttg agcagagagt tggtaaactt tttgatgcgg caatttgaga
      181 aactcgtcaa gcaagccgag ctcgtacatg atttcaagtg tcgatggatg aatggtatcg
      241 ccgcggaagt cgcgcaagaa atccgcgtgt ttttccagca cgatgacatc gactcccgct
      301 cgggcaagca aaaaaccgag catcattccg gctggaccgc ctccggcgat gcaacagcgc
      361 gtgcacagcc tctggtcgag gctttcgttc ggctcaacca ttgccagtca cccgcgctaa
      421 acagcttcca ttctcaaatg atgaactccg acgatcacct cctgacgatt ataagcgttc
      481 ggcatcctaa acgacagcag ctccagatcg ggcggggttg gtttgaatgt aacgcaaggg
      541 cagctctaat ttttgcgagg accggaccca tgtcgtaatc ctatgagaaa gctcttggtc
      601 ttccagcatg ttgctcacga aattctcggc actttaaacc cactgttgaa gcgcgccggc
      661 tttcgtattc gttacgtcaa cttcggacgc cacccggacg cgcaaccgag cttggacgga
      721 tatcacgaac tcgtgatact tggcggccga cgggtgttta ccaagatcat cgcttccaca
      781 cctgacaact gagatgaaat tgattgaaga tgccatgcga ctactgccgg tgctaggcat
      841 ctgccttggc gcgcaattga tcgaaaacgc tcggtgcgga tgtctactca ggcaagcata
      901 aggaaatcgg ctggtatgat ctacacagcg cgcccgaagc cgccgggaac tcgatcttaa
      961 aaccgctgcg cgggaccgag aaggttttcc agtggcacgg cgatacgttt gactcacctc
     1021 cgccgcatct ccgctctgcg tgaaccaggc atttcgctac ggcgacaagg tttacggctt
     1081 gcagtttcac cttgaagttg acgagccgcg ataatcttgc gttgactgaa agtcccggag
     1141 aaccaggacg agatcgccaa gcttccgggt gacgtcacac cggatcacat tcatgcagcg
     1201 acagcgcggc acatcgcgcg cctgcacgaa ctcagcgatc aaacctttgg cgaatttagc
     1261 gcactcttgg gtctggaaaa gatccgctgg cggcgggtgt cgggatgaat tttccgtttg
     1321 cgcaaattag cgattattcg ccggccagct tatgcactaa ttcatacagc gcccgtacgc
     1381 ctacgcgaag gctgtcaatc gagattctct catcgatacc gtgcactaat ccttctgcag
     1441 cgatgttgcg aagtggcata aagcccagac aagggatccc tcgggcgcga aagaagtggc
     1501 agtcggtaaa gccgcgcacc agcggcgcca cgactggagt accatgatcg tgcgttctag
     1561 ccaattcctc gatcgccttt atcgcctccg ggtgggccgg agaaacggcc gcggggaaag
     1621 ataagaggac ttcaatcttt atattctcat ccgcaatcac ccgccgcacc tcgccgataa
     1681 aagcctgagg atcttcgcct ggcagcagtc gaatatcgat atcggccgat gcctgcgctg
     1741 gaatcacatt caccttgtcc gacgcgctca gacgggtaac cgagatggta tttcgcacgc
     1801 tggcattatt acgcgtattg cgcgtgaaat ccgcggcgaa ggccggatct ttgagagcag
     1861 ctcgcaggtc ctggtacttc ccgcggtgac tctcgggctc ttcgccagcg gtgtcggcga
     1921 agaacttctg cacctccggc acgactttga ttggcgtttg ataagctagc agacgtccca
     1981 gggcaagcac cagcttatcc actgctgctt cttttacagg agtcgagcca tgtcccgctg
     2041 cacccgtagc gataagccgc aaccaaagcg gagttttttc ggcgacgctg acgctataac
     2101 tctgaacctt gccatcgctg cccaaccgca ctccgccgcc ttcgttcaaa actaggccga
     2161 catctgcgaa caactccgaa tgtttttcga gtaagaattc agcgccaagc gccccgcctg
     2221 cctcttcatc ggcggtgccg agaaaaatga cgtctcccag aagggcgact ttattgcgct
     2281 tgagtgtcag gagagtcatc agctcggcga tagcggggcc cttcatgtcg agagcgccgc
     2341 ggccccaaat gtctccgttc ttgacggtcc cgctgaatgg ggcttctttc cacagcttgg
     2401 tatcggcggg caccacatcc atgtggttca gcagtattac agctttcttg gcgccgctgc
     2461 cgcgcagccg ggcgtagatg ttgccgcgtc cgggcgcaga ctcgatgatt ctcgcttcga
     2521 ttccttctct gtcgaagatc tgtttaaaaa attgcgccgc cttgatttcg tttccgggcg
     2581 gattggtggt gtcgatttgg atatagtggc tcagtagcga caccgcttct tgttccggcg
     2641 cattgccagg caaagatcct tcggctgcca agctgttgcc gctcaaagat acaaccagca
     2701 gagtcaaccc aaatatcatc gagagcgtgc ggtacccgcg ttttctcacg atccttcctc
     2761 cgagctgcaa acattccata aactgaacac aaggatcagg cataaaattc tctcacggcg
     2821 tcttcgatgt tgacgcgttg ggtttccgga tcaaaccagc gaatttcgcg gtcgctgtga
     2881 aaccaggtga gctgccgctt ggcgaatctt cgcgtgtctc tcttcatcag atgcaccgca
     2941 tgatctaaag atatctgagc ccgaagaaac agcccgaggt gacgataacc gatactttgc
     3001 atcgctttca aatccaagcc atagcccatg ttgacaagct tctcgacctc gtctaacaga
     3061 cccgccccga tcatgaactc gcagcgccgg tcgatgcgat catagagatc actgcgttgc
     3121 cgatccagac ctattttaag cacatcgaat agcaagtcgt taaagccgtg ctcgctctgc
     3181 cactcactga tttttcgccc ggtcgcatag caaacttcca gagcccgggt gattcgctgc
     3241 cggtcatggg gatgaatccg cgctgccgat tcaggatcga cccgctccag ccggcgataa
     3301 agccaatcca ttccatactc ctctatctcc cattcaagcg cctgtctgat ctgcggatct
     3361 cctcccggtc cggcgaacaa tccttgagtg agagctttga tatagagtcc ggtaccgccg
     3421 caaacaatag tcctcttgtc gcgatcggcg agcttgtatg cgatctcggt ggccaggttt
     3481 cgaaacatgg cagcgttgaa ctcctcgtcc ggatcgacga tatcgatcaa gtgatgaggc
     3541 accgtttgtc tttgctcttt agatggcttt gccgttccga tatccatgtg gcggtagact
     3601 tgttgcgaat cggcgctgat gatctctgca tcaaagtgca gggcgagatc cagcacggtg
     3661 tcgcttttgc ccacggcggt aggaccaaga acaatggcga gcttgggttt catggcagca
     3721 acaaacgtat tgggggaatg gctggccaag cggttgcgcg aacgctatga cacgcgttta
     3781 aacatccggt cgagctcttc acggctgaac tcgagcagga ccggccggcc gtgcgggcat
     3841 tgcgtggcga actcggtgtc gtcgagatcc cgcaagagcg cgcgcatctc gtgcatttcc
     3901 agctggcgat tagcccggat gacgctgtga caggcgatgg tcgccaagcg ctcttccaga
     3961 tgctggctaa gcttctcgga ttggtctatc tccgccaact ccgccaccat ctggcgcacc
     4021 accggccggt aatccccttc cggcaacaac gccggaaccg cgatgatcgc ataagagcgg
     4081 gggccaaacg gctcgagcgt aaagccataa cgttccaaga catcgagctg cttttccaac
     4141 atcacacttt cgccggccga taattcgacc gtctggtgca gcaatagtgt ttgtcgttcc
     4201 acggcattgg cgcgcatctg ctgtcttaat ctttcaaagg cgacccgttc gtgcgcggcg
     4261 tgttggtcga gcagcgccaa accccgagga gatgaacaaa ccagataaca gccgagaatt
     4321 tggccaagta cattgagcga agaaaaaaac cctgcgcgcg tttgagccga gtggttcggc
     4381 agaacaaaaa cttcccggcg ggtttctgaa accaaagatt ccaccggatc agagactgaa
     4441 atcgcatacg gcaatgaggc ttccataacc ccagaagaat attgcggcca cggcgcaaag
     4501 ctacgcttcg atggctgttg agcctccgct ctgagagctt ggcgtatcgc gcgagcgacg
     4561 gcatcatgca cgtcggactg ccggcgaaat ctgacctcgt acttggccgg gtgcacgttg
     4621 acgtcgactt cgtcaaacgg aatatccaat agcagcacca ctgccgggta ttggcccttc
     4681 atcaaaagag tttcgtaccc atgcagaacc gcatgtgtga gaatcttatc gcgaacataa
     4741 cggcgattga caaaggtcag cagatgacgc gagtttggaa acgcggtcgg agctgaactg
     4801 aggtaacccg tgacagacag gataccctgc ctaaactcga acggctgtaa ggagtcggcg
     4861 gcttcccttc ccaagacttg gtgcaagcgt tcacgcaact cacgcatgcc aacaaaatcg
     4921 atcagaatct ttccattgtg ttccagacgg aaatgaattt ccggataggc gagcgccagt
     4981 cggttaacga tatcgcagat gtgactgaac tctgtagtga gggatttcaa aaattttcgc
     5041 cgggccggaa cattgtagaa gaggtctttc agttcaacgg tagtgccgat ggaagcggcg
     5101 gtgatatgcg ccgaggttct atctccgccg tccacgcgca ctcggtatgc cgattcatga
     5161 ccggcctgtc gtgaagtaat ctcaaatcga gaaaccgaag cgatgctcgg caatgcctcg
     5221 ccacgaaatc ccaaggtcga gatccggaac agatcgtcgt cgctacgcag tttgctggtt
     5281 gcgtggcgct caagcgcaag cggcagatct tcgggcgaca tgccctcgcc attatcgctt
     5341 acccggatca gcgaggtccc gctcttctca actgatatcg taatttcgct ggccgccgcg
     5401 tcgatggaat tctccagcag ctctttaatc accgatgccg gtcgctcgat cacttctcct
     5461 gccgcgatac ggctcgccgt ttctctcgaa agaacctgaa ttttcacttg atgaccttac
     5521 tcccgtgttc ggggccttag ctgcggtgct gggaaaccgt caactctggt ctaagatttt
     5581 gccaatcgat caatgaaaag ttcgagcata ttgacggtta tggattacag tgatgtcggg
     5641 cggccacgtc gggcgattct ctcactcact cggccggtca cgccaacgat agagtgcatc
     5701 tgtacaagct gagcggtatc gagaccgcag atcaaagtat cagcgatttt cttgtcgaat
     5761 aatctcgtcc agcttcttcc ggtcggtatc cgaaatcttt tccttcgcgc ccgtctcatc
     5821 ggagcgagtg cggcgctcgt ctttattcaa gttgcgcgac ctattggggg cggactccgg
     5881 atgatcgtcg gcgggcgcta aacttcttgt aatctcgttc cagtatagcc aaacgaggaa
     5941 agccaaggcc caaacggtca gtggcgcaag cacgaaaagg agaatggttt tgcctctgct
     6001 tcttttcctt cgcttgcgtt ttgccactgt aaatcgatca cccgatattt actgacaccc
     6061 tgcggcctga tcgaaatcaa gccgaggcaa tcaaccgaac gaagcctacg aggttttaac
     6121 ccgcgtctta gtaattcggc ttgtgctgga aatagtgcgt ggcattgata atgcgaatcg
     6181 tacgagtctt cgaccgcatc accaccgatt gtgttgtcgc gccaccggga aaaaagtgaa
     6241 cgccatcaag ataatcgccg tcggtgacac ccgtggccgc gaacatcacg tcccccgcgg
     6301 ctaattcctc gattgaaaac ttcttcttga tgtcgctaac gcccattttt ttggcgcgct
     6361 cgatttcttc attattgcgc ggcgccaggc gaccttggaa ttcgccgccg acacagcgca
     6421 gcgccgccgc cgcaagcacg ccttcgggcg cgccgccgat gccaagcaag agatcgattc
     6481 cggtttccgg cttggtcgtg gcgattgcgg cggagacatc cccatcgccg atgagccgaa
     6541 tacgcgctcc cgcttggcgc acctctttga taagcgcttc atgtcgcggc cgggataaaa
     6601 tgataaccgt gagatcctcg acgttacagc gcttggcttc ggccagagcc tgaagatttt
     6661 gggtcggcgt tttgttcaaa tcgaccacgc ctttaccggc tggtccgacg gcgatcttct
     6721 ccatataggt atccgggcaa tgcagtaaat ttccgccgtc ggccatggcg attaccgcca
     6781 gagcattagg cgcgcccgta gcgcaaatcg tcgtgccttc cagcggatcc agggcgatat
     6841 cgacttttgg accgccggaa ccgaccttct cgccgatgaa aagcatcggc gcttcatccc
     6901 gttccccttc gccgataacc accgtgccat cgatggggag cgcatcgaac gctctacgca
     6961 tcgcgtccac cgccgcctga tccgccgcct tttcatctcc gcgtcctgtc aaacgagcac
     7021 aactcaaggc ggccgcttcg gtaactcgga tggcttccaa agctaaattt cggtccacgt
     7081 ttgcctcccc tcgattaacg cctccataag tctgaaggct tgcttggcga ccacgccggt
     7141 tttcgcggcg tccttctcat tgtatccgac gctgccgtta ttgcgagctt cttgcggaga
     7201 ataaagcacg aacggcacgg gatcagagac gtgggtctta agcgcgatgg ccgtcggatg
     7261 gtcgggcatc aagagaacac gccaatcttt tcggtgggcc atgcccgtga gtaccgtgcc
     7321 gacaaccttt tcatcgaaat cttctagagc ctgaattttt ttctcaacat cacccatatg
     7381 gccggcctca tcggtggctt cgacgtggat gaaaacgaaa tcgttgttct cgagcgattt
     7441 aaggccgtac tcacctttac cgcgataatt ggtgtccaaa aatccggtga tgccgggcac
     7501 atcaatccgt tcgaggccgg cgtaaacgcc caaaccgtga atgatgtcta ccgccgagat
     7561 aacgccgccc ttgatgccaa aacgcttggt cagagggggc agcgccggcg cgcgcccctg
     7621 accccacaac cagatactag tggctcgtcg ttggccttga gcccgtcgca ttttgttgac
     7681 cgggtgatcg gcaaggatcc catgtgaagc ttccatgagc ttgagcaatc gatcggcacc
     7741 ctcgccctcg ggcataaaac cggaaatttt ctgaccggta atatcgtgcg gcggggtcgt
     7801 tttcatcttt tctttgccgt cgcgccacac catcaagtgc cggtaactga cgccaggaaa
     7861 gaactcgata ccatcagcgc cgagcgcgcc tgccaaatca cctatgatca ttccagcttc
     7921 ttgggtgcta atatgacccg aggtaaaatc ctccatcacc ctatcgcttt cactgccggt
     7981 gagggtcacc agattacagc ggaaggcgat gtcgttcggt ccaagcgcca ctcccatgct
     8041 cgcggcttcc aacggcgatc gtccggtgtg atagaccgcc ggatcgtagc ccatgatgct
     8101 catgttgccg acatcactgc ccggcgggaa gcctttcgga atcacatgag ccaagccaaa
     8161 cacgccgcgc gccgcgagcc agtccatgtt aggtgtcctg gccgcttcga gaggagtctt
     8221 accgccgagc acctcgagcg gatagtccgc catgccatcg ccctgtagaa taatgaattt
     8281 catttcagtg aatttatctt ttgtgaaatc ctgcagaatt tctgcttgta gaaggttcta
     8341 aacatgttag ccgaaaggca acctggagag tcaggtggcg aacaaagtcg ataacgtctc
     8401 tggctcgatt cgttgggctg tgtcagctta cggcccaaat cggaaaccac acgggatcaa
     8461 ctacgatata atctcgatcg cgcaccgaat ccagagcggc acggatttgc cgcaaggtcg
     8521 ctcgctcgag cgtcataacg aatggcaaaa tttccttgaa gtctgctctt agattataac
     8581 tgcgggccag gtcctgcagc tcctcgtcgc cgtgtctgag ctgccagatc tcggcgatat
     8641 tcactcccat agcgccaaag gtttggccga tatcaccgac gatgccgggc cgatctttga
     8701 cgataaaacg aacatagaag gagccagcaa tgtcgtcttg cggctgcagc gacagaaggc
     8761 ccggctgcac caagctcggc atgccggtga accagcccgg tttggctcgc gccaattcac
     8821 agacatcgcc aagcacggcg acagccgttg gaccaccgcc tgcgccagga ccgaccagca
     8881 cataatcgca gtcacgatgg ggacgtatgc cgccgctttt tttaccatta aagcagattg
     8941 cgttagtcgc gccatggatg ccggcgagga gattgtcccg cggcatcaaa accggagaga
     9001 caaacacctg caccgatgaa ccgattcgct tggccaccgc cagatgtttg atcgtgctcg
     9061 caccggttcg cccggcgtaa cggaaatcca ccgcgtgaat atcttcaatg cccttgcgcc
     9121 atgtggtacc gggccggagt tgaagcccga acaccaccga gctcaataac attagcttgg
     9181 cttcggcgtc gctgccgctc acgtccgccg tgggattggt ctcagcatag cctttttgct
     9241 gcgcttcctt gagcgcttcg gcatagggtt tggcggtctt ctccatttgc gacaggatgt
     9301 agttgcaggt gccgttgaca attccaaaga ccgcttcggg ttcctcgacg gcgaagcttt
     9361 cattgagcga tcggatgacc ggaatgccgc cgccaacgca agcttcgaaa cgcaagtgct
     9421 gaccgctttg ctgcgccgct gcccagattt ccgcgccata gcgcgccagc accgctttgt
     9481 ccgaggtcac taccgatttt cctttttcga aagcgcgcag gatgtaatca cggaaaatcg
     9541 gaagatcgcc gggctgctgg aatcccaggg cttctatgac gatttccact gtggggtcgt
     9601 cgatcacatc gattgcttgc gaggtgaaga gctcgggacg atccttgaac cactttttgg
     9661 ttttgggatt tcgagtgtag atcctgcgga gttcgagttc caagtcttgg ccgactatcc
     9721 cgtggaagcc agcgagaaga ctatcctgaa tggcttcgcc gacaacgccg gcgcccaaca
     9781 ggccgacgcc aattttacgc cgtgcagtca tctaaaatgt aaaaagctat cgaattctaa
     9841 ctgagctggt tttggcgcgc gctaatccaa caatttgccg agagcgcgct tgatatttcg
     9901 caccgcctgc ttcgttcgat gctcgttctc gatcaaggcg aaacggacgt ggtcatcgcc
     9961 atactgtcca aatccgatcc cgggcgatac ggccactttg gcttcttgca gaataaattt
    10021 ggcgaattcg atggaaccca tttttttcgc ggcttggggg atttcggccc agacgaacat
    10081 cgtgcccttg ggttttctga tcggccaccc ggcgcgagtg agaccatcga tcaagcaatc
    10141 gcggcgtttt tgatacatcg cgcgaatgtc ttcaacgcaa tcctgcggtc cgtttaacgc
    10201 ttgtatggcg gcaatttgca ccggctgaaa aatcccgtag tcgagatagc tcttgatccg
    10261 cgccaacgca tgaatgatat ctttgttgcc gacggcaaag ccgacgcgcc aacccggcat
    10321 gttgtagctc ttcgaaagcg agaagaactc tacaccaacc tccttcgccc cgggcacctg
    10381 cagaaaactt ggggctcgat agccgtcgaa ggtcaggtcc gcgtaagcca gatcgtggac
    10441 gacgataagt tcgtactctt tggcaaactg gacgatgcgc tcgaaaaaat ccagttcgac
    10501 cacttcagtg gtcggattat ggggaaaact gatgatcaac attttcggct tgggccaggt
    10561 ctgcctgatg gcttcttcga gatggccgaa aaactcctcg ctggtaatta gcggaatgca
    10621 gcgcaaatcc ccgccagcaa gaaccacagc atactggtga atcggataag tcgggctggg
    10681 acaaagcacc acgtcgccgg gatcgatggt ggcccaagct aagtgggaca acccttcctt
    10741 cgcgccgatg gtgacgatgg cttcggcatc cggatcgata gcgacttggt aacgacgctg
    10801 gtaccaatcc gagatcgcaa cccgcagttt gtagatgccg cgcgacaccg aataacgatg
    10861 gttctgcggc ttgctcgccg cctcgataag ctttgccacc acatggggcg gcgtcggcag
    10921 atccggatta cccataccga aatcgacaat gtcttcgccg cggtgacggg cctgcatctt
    10981 catcccatcg atgatgctaa acacgtacgg cggcagccgc ttgatgcgct gaaattccac
    11041 tcgaaactac ctctttccgg taaaaacgct ctggctgctt ttataagcca tcggccattc
    11101 tggcgcaact ttgatctagc caattctgac atgaagcggc gcaaaagaca ccaaatttcc
    11161 agctggtctg gccatttggc cgacagctaa tttcgggatt gttcttcaag aaacaagaca
    11221 caaataacca gatagaacta aatagatgga aacctggctg ctaaggaagt ctgcgagcgc
    11281 aaagcacccg cttagcgctt acgccggctg tctctctcgg gttgacttcg atgcgatagc
    11341 tactctatac ctcttttgcg tcgatggctc ggattccaaa tcacatgttt aggagaaatc
    11401 atgacaaaga cgtttccgat attggtgata tgttcgttta ctttccttgc atcttcggtc
    11461 tgtgcgcagg agcctaaact cattgaagcg gcaaaaaagg aaggcggtaa aatcgtcgcc
    11521 tatggatcac tggaatccga taccaccgag gcggtaaaac aagccttcga gaaaaagacc
    11581 ggtctcagga tggaatattg gcgcgcgtcg gccaccaaag ttgtggatcg agcgaccagt
    11641 gaatttcgcg ctcaaaaacc gctcttcgac gtcattctgg ttcatgatgc cgagatgcgg
    11701 ctcctgttga agaatagtgt tataagccgg tacaactcgc cggccttcaa ggatttcacc
    11761 aaagagtcca tcgatgccga tctcggcccg agataccgcg acaccatcat tggaatcgtc
    11821 tacaacaagt cgatgatcaa gcccgaagat gcgccgaaat ccatggaaga cctgctgcaa
    11881 cccaagttca gaggaaagct ggtcatgccg gacccgactc agcatacgac gacgctgcag
    11941 tgggttgcca gccttgaaaa ggtcatgggg aaagaaaaag ctgatagatt cgtccgtgat
    12001 ctggcggcaa ctaaacctct tttggtcgag tctctccttc cagccgccga gcgcgtcagc
    12061 tcgggagaaa cacccattgc catcacctac attaagttca gctatctatt tggcttaaag
    12121 ggtgcgccgt tggactatgt gagatcggac aaaatgttgg gtgaaggaag ttacataatg
    12181 ctggcgagca agccggctca tgccaatgcc ggcaaagcat ttatcgattt ctttcttgac
    12241 gatgaaagca tgaagatttt ggcgaagaac ggcgaattcg ttacccgtaa gggaatccac
    12301 ccgccgattc caggcgccga gaagataaaa tttattgaaa tgactcattt cgatcagaac
    12361 ggcttcacag agaaaaagaa agaataccag aagatcttct tgcagtaatc gattccgagt
    12421 ggagataagt actcaccaaa acaccattaa ccaccattcg ccgatctggt aggtagacgc
    12481 gacacgctcg cgtctacccc cccagcaaca ccactgacgt cacttagaag aacggcctcc
    12541 gggtcggaac acatcgtcga ccggtttggg tgatacaaag gaatagaaag caagacaacg
    12601 atatgcgaga agaagacgcg agctggagta ttctgcttga atttttagat cgagtcctaa
    12661 gcttttacgt atgacttgga agcgaatcaa agaaattaca aactctgcac aaaatcgatt
    12721 atcaatcgat tgacgtgtct tgcctgttct cggaagacat aatgacctgc atgattgaaa
    12781 gcatggaatt cggtcctatc taccactgaa ctgatcacgt gaagcaagtc gattcccagc
    12841 ttcatggggg aggattgatc gttgattccc caaatgatca acgtcggagc cttcaatcct
    12901 ccggcttgaa tcagcttcag ggtctcatac tttttctcgc gcatactcgg aagaaacact
    12961 tcatccagaa aacgctccat cttcttgcgc gcctcgagcg tccttgggag ccgacctatt
    13021 ctcagcatct cttcaacgaa atcttcagtg atgtggtcat gcgagtagga gttggcgatg
    13081 ggctctctga gaataaattc tcgatccgga acggcaggcg cgccgacttc cagtttcgta
    13141 tagaaatcat agggcaatga cgcatcttcc gcagggagag tgttgctgtc aaaaatgatc
    13201 agggctttaa ccaattcggg gtgatcgacc gccagtcgcg ccgccggcag agctcccctg
    13261 gaatg
//
LOCUS       DASUVS010000280         3051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1207680, whole genome shotgun sequence.
ACCESSION   DASUVS010000280 DASUVS010000000
VERSION     DASUVS010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207680"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VF182_15510"
     CDS             <1..684
                     /locus_tag="VF182_15510"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6438544.1"
                     /translation="GMVMIGAIYLENAPVNLSMVTPVARLTGEYEMIVVPAKSPHKTM
                     GDLVKTFKANPGGVSWGGGSAGGTDHILVGLIAKAAGVDPGKINYVPFKGGGEAIAAI
                     VGGHVTAGVSGIGEFAEHVKGGRMRALAVSSASPIDNVPTLKEQGLNVELTNWRGVFG
                     APGITAAQRDALIKIVRGATESATWKKTLEKLGWSPIFLSGEDYKKFIDEDTKRIAAI
                     IDSLGIKNK"
     gene            696..1196
                     /locus_tag="VF182_15515"
     CDS             696..1196
                     /locus_tag="VF182_15515"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX6438545.1"
                     /translation="MKAKPAELVLSLAVLSLGIAVAVGTASLSGAGGYARIGPNVAPA
                     VVAAGLILLGLWLLYEAAFGGWRNAAPESPEARGEHEFSPSAFIWVSVGLITQMLLIH
                     HAGFVIAQAALFTCVARGFGSKRYLRDFAIGLALGAAVFLFFVKFLNVNLPPGWLAPI
                     MRGAGI"
     gene            1203..2699
                     /locus_tag="VF182_15520"
     CDS             1203..2699
                     /locus_tag="VF182_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013169097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6438546.1"
                     /translation="MAETFSLLLQGFSVALTPLNLLWGFIGVMLGTFIGVLPGVGPAL
                     TVAMLMPLTIRLDPTGALIMFAGIYYGAMYGGSTTTILLNTPGESASIATALEGNLMA
                     KKGRAGPALATAAIGSFVAGTIATVLVTLLAPVVVEVALKFGPAEYFALMIFAFTTVS
                     AVIGSSTVRGLTSLFLGLLLGVIGIDSQTGRSRFAFGVPELLDGVDVVVLAVGLFAVG
                     EALYVAAYTSRLEEKLEQLKGSLWMSKEDWKRSVPAWLRATVIGFPFGSIPAGGAEIP
                     TFLSYATEKRLSKHPGEFGKGAIEGVAGPEAANNASVTGALVPLLTLGIPTSATAAIL
                     LSAFQNYGIQPGPLLFQTQADLVWGLLASLYVGNVLLLILNLPLVGIWVKVLAIPRPL
                     LYGGILVFATLGAYSLHQSAVDLMILYVFGLLGFVMRRWDIPVAPAVIGLILGPLAET
                     QARRALAISQGDPMVFLTQPISATILSVSLLLLIVPVVVSISRRDRSP"
BASE COUNT          565 a          849 c          924 g          713 t
ORIGIN      
        1 ggaatggtca tgatcggtgc catctacctc gagaacgctc cggtcaatct tagcatggtg
       61 acgcccgtgg cgcgccttac tggcgaatat gaaatgattg tcgtgccggc aaaatcaccg
      121 cacaaaacta tgggcgatct agtgaaaacc ttcaaagcca atccaggcgg cgtctcctgg
      181 ggcggcggct ctgcgggcgg caccgatcac atactagtgg gtttgatcgc caaagccgcc
      241 ggcgtcgacc caggcaaaat taattacgtg ccgttcaaag gcggcggcga ggcgattgcc
      301 gccatcgtcg gcgggcacgt gaccgccggc gtctcgggga ttggtgagtt tgccgaacac
      361 gtaaaaggcg gccgcatgcg cgctctggca gtctcgagcg cgtcgccaat agacaatgtg
      421 ccgacgctta aagaacaggg cctgaatgtc gagcttacca actggcgcgg cgtgttcggc
      481 gctcccggga tcactgcggc ccagcgtgat gctctaataa agatcgttcg cggcgccacc
      541 gaatccgcaa cgtggaagaa gaccctggaa aaacttggct ggtcgccgat ctttttgagc
      601 ggcgaagact ataaaaaatt catcgacgag gacactaaac gcatcgccgc gatcatcgat
      661 tcgctcggca ttaaaaacaa gtagccgttt cactcatgaa agccaaaccg gccgagctgg
      721 tgctatcgct ggccgtcttg tcgttgggaa tcgcggtggc cgttggcacc gcaagtttgt
      781 caggcgccgg cggctacgcg cggatagggc ccaacgttgc cccggcggtg gttgccgcgg
      841 gacttatcct gttaggatta tggctgctct atgaagccgc ctttggcggc tggcggaatg
      901 cggcgccgga gagtcctgag gcccgcggcg agcacgagtt ttcgccctcc gcttttatct
      961 gggtgagcgt cggcttgatc acccagatgc tgctaatcca ccacgctggc tttgtgatcg
     1021 cgcaggcggc tctgtttact tgcgttgccc gaggcttcgg cagcaagcga tacctgcgag
     1081 atttcgctat tggcctcgcg ttgggcgcgg cggtctttct tttcttcgtc aaattcctca
     1141 acgtcaatct cccgccgggt tggttagcgc cgattatgcg cggcgccggg atctagcttt
     1201 ccatggctga aaccttcagc ttgcttctcc agggttttag cgtcgcactg acgccgctca
     1261 atctgctatg gggattcatc ggggtgatgc ttggaacctt catcggcgtg ctgcccggag
     1321 taggccctgc tctcacagtg gcgatgctca tgccgctgac catcaggctc gatcccacgg
     1381 gagcgctgat tatgtttgcc ggtatttact acggcgctat gtacggcgga tccaccacga
     1441 cgattctact caacacgccc ggagaatcgg cctccatcgc taccgccctg gaaggcaatc
     1501 tgatggcaaa aaagggtcgg gccggcccgg cgcttgccac cgctgctatc ggctcattcg
     1561 tcgccggtac catcgcgacc gtgctggtga ccttgcttgc tcctgtggtt gtcgaggtcg
     1621 ccttgaagtt cggccctgcg gagtattttg ctttgatgat ttttgcattc accacggtat
     1681 ccgcggtgat cggtagttcg accgtgcgcg gcttgaccag tttgtttctt gggctcctgc
     1741 tcggtgtgat cggcatcgat agccagaccg gcaggtctcg atttgccttc ggcgtgcccg
     1801 agttgcttga cggtgtcgac gttgtcgtcc tcgccgtcgg attattcgcc gtaggcgaag
     1861 cgctttacgt tgccgcatac accagccgtt tggaggaaaa gctcgagcag ttgaaagggt
     1921 cgctgtggat gtccaaggag gactggaaac gatcggtgcc ggcgtggctg cgggcgacgg
     1981 ttatcggctt tccgttcggc tctatacccg caggcggcgc tgagatcccg acgtttctgt
     2041 cttatgcaac tgaaaaaaga ctgagcaagc atcccggtga attcggcaag ggcgccatcg
     2101 aaggcgtcgc cggtcccgag gccgcaaaca acgcatcggt aaccggcgcg ttggtaccgc
     2161 tgctcacgct gggcattccg acgtcggcaa ccgccgcaat cttgctcagc gcctttcaga
     2221 actatggcat ccagccgggt ccgctgctct tccaaaccca ggcagatctg gtgtggggat
     2281 tgctggcaag cctttatgtc ggcaatgtcc ttttgcttat cctgaatctt ccgcttgtgg
     2341 gcatttgggt gaaggtgctg gcgattccgc ggccgttgct ttatggcgga atcttagttt
     2401 tcgctacact gggcgcctat agtctacacc agtccgcggt cgatctgatg attctttatg
     2461 tctttggact gctcgggttc gtgatgcgcc gttgggacat tccggttgcg ccggcggtga
     2521 tcggactgat tcttggtccg ctggctgaga cgcaggcccg gcgcgcgctc gctatcagcc
     2581 agggtgatcc gatggtcttt ctgacgcaac ctatctcagc gacgatcttg agtgtttctt
     2641 tgttgctgct gattgttccg gtcgttgtca gtatttcgcg gcgagacaga tcgccgtaaa
     2701 tccctcgccc aacatccatt cgggtaaatt gctaaagaac ttggagtcag cttgcggaaa
     2761 agtggttctg gcaggctgtt caaaagatct cacaggcgag gcccgcgaag ttcgatgagc
     2821 tgaggcgtac gcttcagtac gttgaagcga atcgggcgag cggaacgaag catatgaggc
     2881 ttttcagcag cctgctacgt tttctccaga ccgatagaaa acatgcgctc gagatcatgt
     2941 cgtgaataaa cgcggaaggc gatcagggtc tcggagctgg tgatacccgg caatgcgcgg
     3001 acatgttcgg tgacaatgtc ggcgagctga tcattgtctg aagcacggac g
//
LOCUS       DASUVS010000281         3305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_551761, whole genome shotgun sequence.
ACCESSION   DASUVS010000281 DASUVS010000000
VERSION     DASUVS010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551761"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VF182_15525"
     CDS             <1..453
                     /locus_tag="VF182_15525"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438547.1"
                     /translation="KQSLEATLAASRSLKIQVQVLEIQSVNEFDGAYSASKKNNAEAI
                     NVLASPILFAYRQDLVNLSATHRLPGMYENKEFVEIGGLMSYGANLDELYRRSATYVD
                     KILKGAKPSDLPVEQPTKFELVINLKTAKQIGLKIPPNVLARADRVIK"
     gene            450..1424
                     /locus_tag="VF182_15530"
     CDS             450..1424
                     /locus_tag="VF182_15530"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438548.1"
                     /translation="MKLIIVAVCTLIFALCFLADAQESTRIPRIGVLVAGQPPTRPSL
                     EGFRQGLRELGYVEGKNIHLELRWDEGNPDRWAEMASELVRLKVDVILAGHGVAALGA
                     KQATTTIPIVIGATSDPVAAGLVRSLARPGGNITGLAFYGSELGPKRLELMKETLPKL
                     SRIAMIWHPFGLNLDIVVKDVKATAQSLGLTLHPLEVRQRDDLESAFEAARQKQAGAV
                     LISQGAFFGTHRAYIAERGLTHRLPTISGEAEFAEAGGLMFYGQNIPNTWKRAASYVD
                     KIIKGARPGDLPIEQPTKFELVINLKTAKKIGLPIPPNVLARADRVIQ"
     gene            1421..2440
                     /locus_tag="VF182_15535"
     CDS             1421..2440
                     /locus_tag="VF182_15535"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438549.1"
                     /translation="MKAGNKQQQLADLNRSRLIASAAIGLLYALCSSANAQQPKKIPQ
                     IGYLNVVSVSAGTGRIEAFRQGLRELGYLEGQNIVIEQRHAADRADRLSAFAAELVDR
                     RVDVIIAAGSQAVHAAQKTTRTIPIVMTGSSDPVGTGFVVSLARPGGNITGLSLLSPE
                     LSGKRLELLKEVVPITHVGVLWNPSDPPAALSLRETETAALGQGIKLQTIEAREPDEL
                     GRALTSANKGRVEAILILPAPMMTTYAKRIAEFTIGSRLPAISFSNEFPQAGGLMSYG
                     ANFLDLFRRAASYVDKILKGAKPADLPVEQPTKFELVINLKTANQIGLTIPPNVLARA
                     DRVIK"
     gene            2469..>3305
                     /locus_tag="VF182_15540"
     CDS             2469..>3305
                     /locus_tag="VF182_15540"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438550.1"
                     /translation="MEGILTGSHSRINLKSAFRNRKSAIILCSLLLTPAFTADAQQSK
                     KVPRIGYLLPGNPASESTVSEATRLALRELGYIEGQNIATENRYAEGKRDRFPELAAE
                     LVRLKLDVLVVAGGNTPVRAVKNATQTIPIVMTGGSDPVEAGLIASLARPGANVTGLT
                     NLARELGGKRLELLKEAVPKLARVAVLYDPVNPSHVREVKEVLPMTAGALKLTIQPWE
                     VPDVDGFERAFATFNKERPDGLYVLSGTRVWAIYKRIAGFALKSRLPSIYNNTEAVDA
                     GGL"
BASE COUNT          790 a          856 c          944 g          715 t
ORIGIN      
        1 aagcaatcgc ttgaagcgac gctcgccgcc tcccgatcgt tgaaaataca ggttcaagtt
       61 ttggagatcc aatcggtcaa tgagttcgat ggagcatatt cagcttcaaa gaagaataat
      121 gcggaggcga ttaacgtatt agcctctccc attcttttcg cttaccggca agacctggtg
      181 aatttatcgg cgacacatcg attgcctggt atgtacgaga acaaagaatt cgtagagatt
      241 ggcgggctaa tgtcttacgg cgcgaatctt gacgagctct atcgccggtc tgccacttat
      301 gtcgacaaga ttctgaaggg cgcaaagcca tccgatctgc cggttgagca gccaaccaag
      361 ttcgagctgg tgatcaatct caaaaccgcg aagcagatcg ggctgaagat tccaccgaac
      421 gtgttggcac gggcagatag agtgattaag tgaaactgat tatcgtcgcg gtttgtactt
      481 tgatttttgc gctgtgtttc cttgccgacg ctcaggaatc cacaagaatc cctcggatcg
      541 gtgttctggt agcgggccaa ccgccgaccc gcccctcgct cgaaggcttt cgccagggac
      601 tgcgcgagct ggggtacgtg gaaggcaaaa acattcatct tgaactgcgc tgggatgaag
      661 gaaatccaga tcgatgggcg gagatggctt ctgagcttgt gcgcctaaaa gtggacgtga
      721 ttttggctgg gcacggagta gcggcgcttg gcgcgaagca agcgacgaca acgattccga
      781 tagtcatcgg ggcgaccagc gatccggtcg cggccggact ggtgagaagc ttagcgcggc
      841 cgggtggaaa tatcaccggg ctggcctttt acggatctga gttaggcccg aagcggctcg
      901 agctaatgaa agaaactctt ccgaagctct ctcgcatagc aatgatttgg cacccattcg
      961 ggctaaatct ggacattgtc gtgaaagacg taaaggcgac ggcgcagtcc ttgggtttga
     1021 cgcttcatcc attagaagtg cgtcagcgag atgacctgga aagcgcgttt gaagctgcgc
     1081 gccaaaagca ggccggcgcc gtgttgattt cgcagggagc attcttcggc actcatcgcg
     1141 cttatatcgc ggaacgcggg ctaacgcacc gactcccgac gatttcggga gaagctgagt
     1201 ttgccgaggc gggtggcctc atgttttacg gtcagaacat tcctaatacc tggaaacgag
     1261 ctgcttcgta tgtcgacaag attatcaaag gcgcgagacc cggtgatctt ccgattgagc
     1321 aaccaacaaa gttcgagctc gttatcaatc tgaaaaccgc caagaaaatc ggtctgccaa
     1381 ttccgccgaa tgtgctggcg agagcagacc gggtgattca gtgaaagcgg gtaacaagca
     1441 acagcagcta gccgacctta atagatcgag gctcattgct tctgcagcta tcggtctact
     1501 ttatgcgctt tgttcttccg ccaacgcgca gcagccgaaa aagattccgc agattggata
     1561 cctcaatgtg gtctcggtat cggctggaac tggccggatt gaagccttcc gccagggcct
     1621 ccgagagctt ggctatttgg aagggcagaa tatcgtcatc gagcagcgac acgcagccga
     1681 ccgagctgat cgactcagcg ctttcgcagc ggaattggtc gatcgtagag ttgacgtcat
     1741 catcgcagcg ggatcgcagg cagttcatgc cgcccagaaa acgactcgga ccattcccat
     1801 cgttatgaca ggcagtagcg atcctgttgg aactggtttt gtcgttagtc ttgcacggcc
     1861 gggtggaaac atcactgggc tgagcctgct ttcacccgaa ctaagtggaa aacggcttga
     1921 gctgctcaag gaagtcgtac caattaccca tgtcggcgtt ttgtggaatc ccagtgatcc
     1981 acccgcagca ttgtcattga gagaaacgga aaccgcggcg ctcgggcagg gcattaagct
     2041 acaaacgata gaagcacgag aacctgatga gctaggtcgc gcgcttacat cagctaacaa
     2101 gggtcgtgtg gaagctattc taattttgcc ggcaccgatg atgactactt acgccaagcg
     2161 cattgcggag ttcacgattg gcagcaggtt gccggcgatt tctttttcca acgagtttcc
     2221 gcaggcaggt gggttaatgt cttacggcgc gaattttctg gacttatttc ggcgtgcggc
     2281 gtcctatgtg gacaaaatcc taaaaggcgc aaaacccgct gatctcccgg ttgaacagcc
     2341 gacgaaattc gagctggtaa tcaatctgaa aacagcaaac cagattgggc tcacgattcc
     2401 accaaatgtg ctagcgcggg cggacagagt gatcaagtga ttgcgacttc gaattgagga
     2461 cagaaacgat ggaaggaatt ttgaccgggt cgcactcgcg catcaatcta aaatccgcat
     2521 tccgcaatcg taaatccgca atcatactgt gctccctgct cctgactccg gcctttacag
     2581 ccgacgcgca gcagtcaaag aaagtccctc ggataggata tctattgccg ggcaaccccg
     2641 ccagtgagtc caccgtttcc gaggcaactc gactggcgct gcgcgagctt ggttacatag
     2701 aaggacagaa catcgccaca gagaaccgat atgcggaggg gaagcgcgat cggtttcctg
     2761 agcttgcggc cgagcttgtg cgtctcaagc ttgatgtcct cgtggtggca ggagggaaca
     2821 caccggtccg ggcggtcaag aatgcgaccc aaacgattcc aatcgttatg acgggcgggt
     2881 ccgatcccgt cgaggcaggg ctgatcgcaa gcctcgcccg tcccggcgcc aatgtcaccg
     2941 gccttacaaa ccttgcgaga gaactggggg gaaaacggtt ggaactgctc aaagaagccg
     3001 ttcccaaact tgcccgtgtc gcggttctct acgatccggt caatccttcc cacgtacgcg
     3061 aggtcaaaga ggttctccca atgacagcgg gtgcgctgaa gttgactatt cagccttggg
     3121 aggtgccaga cgtcgacggt ttcgagcgag cattcgccac gtttaacaag gagcgcccgg
     3181 acggactgta cgtgctctcg ggcacacgag tgtgggctat ctataaacgg atcgcgggct
     3241 tcgcgttaaa gagtcggtta ccgtcgatat acaacaacac agaagctgta gatgccggcg
     3301 ggctc
//
LOCUS       DASUVS010000282         7955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1862816, whole genome shotgun sequence.
ACCESSION   DASUVS010000282 DASUVS010000000
VERSION     DASUVS010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7955
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862816"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="VF182_15545"
     CDS             <1..1414
                     /locus_tag="VF182_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin oxidoreductase"
                     /protein_id="HEX6438551.1"
                     /translation="NPQHPASLGGTNPFIQASILSLYDPERSQVVRQLSGITTWEDFV
                     RTARARLESLQPAGGAGLRLLTGNVTSPTLAAQIRNLLKQFPQAKWHVYEPVARDNTR
                     AGTRLVLGQDTDSLYRFDRADVILSLDSDFLFGEPGSLRYARDFSKRRRVRGEQSEMN
                     RLYVAEPTPSITGSMADHRLAIAAGRIGLLAEAVARRLGAKQSSAEDPQELAPYKSWI
                     ETVAGDLQKHRGSSLVLAGEYQPPQVHALAQSMNQLLGSVGSTVVYTEAIEESAGASP
                     LPELVRDMAQGTVDALFIIGANPAYSAPADLNFAEALSKVGFRAHFAEYEDETSALCQ
                     WHVPQAHFLETWSDARSFDGTVTIMQPLIAPLYGGRSAHELLAALIGSVEISAYDIVR
                     GFWRGRVGTAGFDAWWQRALHDGFIPDSGAKLKSAAVHTQTASPSQPGAAAAKVDGLE
                     LIFRPDPTIWDGRFANNAWL"
     gene            1430..2602
                     /locus_tag="VF182_15550"
     CDS             1430..2602
                     /locus_tag="VF182_15550"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6438552.1"
                     /translation="MSKITWDNAALISPRTAQRLTLHNGDVVELRLGDSSVAGPVWIN
                     PGQADESVTVHLGYGRARAGKVGSAIGFNAYPLRTTRQPWFAGGLQINKTGRRHELVT
                     TQGHHTMAGRPLVRSGTLEQFRQHPEFVHEMAHEPPPDLSLYPEHKFDGSAWAMVIDT
                     SVCIGCNACVVACQAENNIAVVGKEEVARGREMLWLRVDRYYDGPAENPDLLFQPVPC
                     MQCENAPCEPVCPVGATVHSTEGLNDMVYNRCVGTRYCSNNCPYKVRRFNFYQYADFR
                     TEVRKLGYNPDVTVRSRGVMEKCTYCVQRINHGRIEAKKENRPVRDGEVITACAQACP
                     TEAIIFGDMSDAGAAVSKLKKDPLNYAMLAELNTRPRTTYLARLKNPNTDLGVNKK"
     gene            2599..3975
                     /gene="nrfD"
                     /locus_tag="VF182_15555"
     CDS             2599..3975
                     /gene="nrfD"
                     /locus_tag="VF182_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX6438553.1"
                     /translation="MTEPLTEARTRDGRYEVIGPGHTAGSITDKISEIVLTRKTPKGW
                     WIGFGISFLLMNLLFFAVAHLLINGIGIWGVNVPVAWGFAIINFVWWIGIGHAGTLIS
                     AILLLLRQRWRTSINRFAEAMTLFAVACAGLYPLLHLGRPGFAYWLFPYPNTMGIWPQ
                     FRSPLVWDVFAVSTYATVSLIFWYIGLIPDLATLRDRARHRFARMAYGMLAMGWRGSA
                     VHWHRYETAYLLLAGLATPLVVSVHSIVSLDFAVSVLPGWHSTIFPPYFVAGAIYAGF
                     AMVLTLAIPLRAAYRLEDLITMHHLDNMAKVMLTTGLFVFYGYIMEAFFAWYSANPNE
                     AYLMTNRATGPYAPMYWLLLICNGLVPQLLWSPRLRASVPLLFVISLVVSLGMWLERY
                     IIVVTSLHRDFLPSSWGMYSGTIWDWATFIGTLGLFLSLLFLFIRVLPMISIFEMREL
                     VHEEREES"
     gene            4006..4515
                     /locus_tag="VF182_15560"
     CDS             4006..4515
                     /locus_tag="VF182_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEX6438554.1"
                     /translation="MAEFDNPEMLCAGAERAHAAGYRRMDAYSPFPIEGLAEVIGFKR
                     TQLPLIVLIGGLLGGIGGFYLQYWISAIDYPMIIGGRPYNSWPAFIPVTFETTILGAA
                     LAAVLGMLGLNGLPMPYHPVFNVERFALASRDRFFLCIEAADPKFELDSTVEFLRSLN
                     PSSIHEVEP"
     gene            4512..5132
                     /locus_tag="VF182_15565"
     CDS             4512..5132
                     /locus_tag="VF182_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6438555.1"
                     /translation="MTEWTERRAAMLIKAKLFKTSLLIAIITIFASAGCRQRMADQPY
                     QRPLEPSNFFDDGMASRPVVPGTVARRGNDGDKQPLNGKVDGKLVDTFPFQVTMEVLA
                     RGQERYEIFCSPCHDRLGTGQGMIVRRGFPRARSLHETRLRDAPIGHFFEVITQGFGP
                     MPSYANQLTEHDRWAVIAYIRALQLSRNVRLDQLPPEDRAKMKETQ"
     gene            5129..6301
                     /locus_tag="VF182_15570"
     CDS             5129..6301
                     /locus_tag="VF182_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438556.1"
                     /translation="MNDPVTNSDGSIQGLARLAFIAGAAGAVISVIGGLFDSAQFFRS
                     YLVAYIFWLAIPLGSLAFLMLHFLVGGGWGVMIRRLLESATRTLPLLALLFVPLLFGL
                     QAVYPWASPGHVAEDEILRQKSAYLNVPFFIARTILYLGLWGVLGYFLNRWSREQDTS
                     AELPAGKRMRRLSGPGLLIYLFTMSFASVDWIMSIEPHWYSTIYGFIWIVNQGVAAMA
                     FAIIGLAWLGAREPLATPLRPGHFQDLGNLLLAFIMLWAYMVFSQYLIIWSGNLAEEI
                     PWYLARTAGLWRWLPPLLVIFHFFVPLFLLLMRDVKRDPNVLVWVAAGVLVMRLLDTY
                     WMIVPGFENSALHWLDATLLLGIGGLWLGVFLWQLQKMPLLPLHVALLAEEANTHG"
     gene            6294..6701
                     /locus_tag="VF182_15575"
     CDS             6294..6701
                     /locus_tag="VF182_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438557.1"
                     /translation="MADAHQLKYETEDFTPRGVRWFTAALAVLAVMVLALMGGLLWVL
                     SDGRRPGQGIVTPGVPAPNMSPAPNLQVASSLDYQQMRAAEEAQLHSYRWVDRESGVA
                     AIPIERAMEILAAGGLPAQKPQAKSPQSKREQP"
     gene            6698..7558
                     /locus_tag="VF182_15580"
     CDS             6698..7558
                     /locus_tag="VF182_15580"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX6438558.1"
                     /translation="MKHVGRGFSAAILAFALSQIVAPETAYPHDEKAARIAADNQPAI
                     FKNIGIEQKLGTKLPLKTIFRDQTGSSISLQDYLSAKPAVLVFSYLECPMLCPLLLEG
                     LVRSLKPLSLDAGRDFDVVVISIDERDTPELAQKKKLEIINRYNRDSSGAGWHFLTGE
                     KSAIEEVTRSAGFSYAWDEASKQYVHASGIFILTPKGEIARVLYGIDYAPRDVRLALV
                     EAGDGTIGTAIDQLLLYCYHYNPLTGKYGFVIMGALRLAGISTVLAMATFIITMLRRE
                     RRGSHGEAEA"
     gene            7561..>7955
                     /locus_tag="VF182_15585"
     CDS             7561..>7955
                     /locus_tag="VF182_15585"
                     /inference="COORDINATES: protein motif:HMM:NF014809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HEX6438559.1"
                     /translation="MFEDLPLFPVRASTFAAQVDTLYFFLVAMATFFVVLIFGTVIFF
                     TAKYRRRSATERPPPIHIDMRLEILWTIIPLILVMISFGWGASLFVQMSQAPEDALEL
                     RAVGRQWMWKLQHPSGAREINQMHVPVGKP"
BASE COUNT         1527 a         2305 c         2325 g         1798 t
ORIGIN      
        1 caacccgcag catccggcga gtctgggggg caccaaccca tttatccaag cttcgattct
       61 aagtctttat gatccggaac gttcgcaagt cgtgcgacag ctgagcggca tcaccacctg
      121 ggaggacttc gtccgaacgg cgcgcgcacg gcttgaatcg ctacagccgg cgggaggagc
      181 gggtctgcga ttactcacag gcaacgtcac ctcgccgact ttggcagcac agatcaggaa
      241 cctgctcaag caatttcccc aggctaagtg gcacgtttac gaaccggtgg cgcgcgataa
      301 cacgcgcgca ggtacacggc tggttctcgg ccaggacacc gacagtctct accgtttcga
      361 tcgcgccgat gtgattctct cgctggactc ggattttctt ttcggcgagc ccggctcact
      421 tcgctatgcg cgtgattttt ccaagcgccg acgagtgcgc ggcgagcagt cggaaatgaa
      481 ccggctctat gtcgccgaac cgacaccgtc gatcacgggt tcgatggcag atcaccgcct
      541 cgccatcgcc gccggccgga tcggattgct cgccgaagct gtggcgcgcc gccttggcgc
      601 caaacaatcc agcgccgaag atccacaaga acttgcgccc tacaaatcct ggatcgagac
      661 cgttgccggt gatttacaaa agcatcgggg ttcgagcctg gttcttgccg gcgaatacca
      721 accgccgcag gtccacgccc tggcccagtc gatgaaccag ctgctcggca gcgtcggcag
      781 caccgtggtt tataccgagg ctatcgagga gtcggcgggg gcaagcccgt tgccagagct
      841 cgtccgcgac atggcgcaag gcacggtcga cgcgctcttt atcatcggcg ccaacccggc
      901 ttacagcgcg ccggcggatc tgaatttcgc cgaggcgctt tccaaagtcg gtttccgcgc
      961 gcattttgcc gagtatgaag acgaaacctc ggcgctatgc caatggcatg ttccgcaagc
     1021 tcattttttg gagacttgga gcgatgcccg gagctttgac ggcaccgtaa ctatcatgca
     1081 gccattgatc gcgccgctct atggcggacg ctcggcgcac gagctgttgg cggctcttat
     1141 cggtagcgtg gaaatatccg cctatgacat cgtccggggc ttctggcgcg gccgcgtcgg
     1201 cacagccggt tttgatgcct ggtggcaacg cgctttgcat gacggattta tccccgatag
     1261 cggagcgaag cttaagagtg ccgcggttca cacgcagact gcgtccccat ctcagcccgg
     1321 tgctgctgcg gcaaaggtcg acggcctcga attgatattt cgacccgacc cgacgatttg
     1381 ggatggccgt ttcgccaaca acgcgtggct gtaggaactg cccggacctt tgagcaaaat
     1441 cacctgggac aatgcggcac tgatcagccc gcgcacggcg caacgactga cattgcacaa
     1501 cggcgatgtt gtcgaactcc gcttgggcga ttcttcggtt gccggccccg tttggatcaa
     1561 tcccgggcag gccgacgaat cggtcacggt gcatctggga tacggccggg cccgcgcagg
     1621 caaggtgggc agcgccatcg gcttcaatgc ttatcccctg cgtaccaccc gccaaccgtg
     1681 gtttgccggt ggtctgcaga ttaataaaac cggcagacgc cacgagctgg tcaccactca
     1741 aggccatcat acgatggcgg gaaggccgct ggtgcgcagc ggcaccctgg aacaattccg
     1801 gcagcatcca gaattcgtcc atgagatggc ccacgaaccg ccgcccgact tgtcgctcta
     1861 tcccgaacac aagttcgacg gcagcgcttg ggcgatggtg atcgacacca gtgtatgcat
     1921 cggctgcaac gcttgcgtcg tcgcctgcca ggcggaaaac aatattgcag tggtcggcaa
     1981 agaggaagtt gcccgcggcc gcgaaatgct ttggttgaga gtggatcgct actacgacgg
     2041 gccggcggaa aaccccgacc ttttatttca gccggtgccg tgcatgcaat gcgagaacgc
     2101 gccctgcgaa ccggtttgtc cagtgggcgc caccgtccac agcaccgaag gtctaaacga
     2161 catggtttac aatcgttgtg tcggcacgcg ctactgttcc aacaactgtc cttacaaagt
     2221 tcggcgtttt aatttttatc aatatgccga tttccgaacc gaggtgcgca agttgggtta
     2281 caaccccgac gttaccgtaa gaagccgcgg cgtcatggag aaatgcactt attgcgtgca
     2341 gcggatcaat catggacgca tcgaagccaa aaaggaaaat cgaccggtac gcgatggcga
     2401 ggtcatcacc gcatgcgcgc aggcttgtcc caccgaagcg atcatcttcg gcgacatgag
     2461 cgatgccggc gccgcggtgt caaagctgaa gaaggatccg ctcaactatg cgatgctcgc
     2521 cgagctcaac acccggccgc ggacaactta tctggcgcgg ctcaaaaatc ccaatacgga
     2581 tctgggagtc aacaaaaagt gacggagcct ctgaccgaag ctagaacacg cgatggtcgc
     2641 tacgaagtta tcgggccggg ccataccgcc ggttcgatca ccgacaagat cagcgagatt
     2701 gttctcaccc gcaaaacacc caagggctgg tggatcggtt tcggtatctc gtttctttta
     2761 atgaatctcc tgtttttcgc ggtcgcccat ctgttgatta acggcatcgg catctggggc
     2821 gtcaacgtgc cggtggcctg gggctttgcg attatcaatt tcgtctggtg gattggtatc
     2881 ggccatgcgg ggaccttgat ctcggcgatc ctgttgctgc tgcggcagcg ctggcggacc
     2941 tcgattaacc ggttcgctga agcgatgacg cttttcgccg ttgcttgcgc cggactctat
     3001 ccgcttctcc atcttggccg tccgggattt gcctactggt tgtttcccta tccgaacacc
     3061 atgggcatct ggccacaatt tcgcagcccg ctggtctggg atgtttttgc ggtctcgacc
     3121 tacgccacgg tttctttgat cttctggtac atcggcctga tcccggacct ggcgaccttg
     3181 cgcgaccgcg cccggcaccg tttcgcgcga atggcgtatg gcatgctggc gatgggctgg
     3241 cgtggctccg cggttcattg gcatcgctat gaaaccgctt acctgctgct cgccggcttg
     3301 gcgacaccgc tggtggtgtc ggttcacagc atcgtcagct tggattttgc cgtgagtgtg
     3361 ttgccgggct ggcattcgac gatctttccg ccctacttcg tcgccggagc gatctacgcc
     3421 ggctttgcca tggttctgac tctggcaatc ccgctgcgcg ctgcctaccg gcttgaggat
     3481 ctgatcacga tgcaccacct tgataatatg gccaaggtga tgctgaccac cgggcttttc
     3541 gttttttacg gctatattat ggaggcattc tttgcttggt acagcgccaa cccgaacgaa
     3601 gcctacctga tgaccaatcg cgccaccgga ccgtatgcgc ctatgtattg gctcctgctg
     3661 atttgcaatg gactggtgcc gcaactcctg tggtcgccgc ggctgcgcgc ttcagtgccg
     3721 ctcctgtttg taatctctct tgtcgtcagc ctcggtatgt ggctcgaacg ctacatcatc
     3781 gtcgtcacca gccttcatcg cgattttctg ccttcgtcct ggggcatgta ttcgggaacg
     3841 atttgggatt gggcgacgtt tataggcacc ctgggtcttt ttctttcgtt gctctttctt
     3901 tttattcggg tgctgccgat gatctcaatc ttcgagatgc gggaactggt gcatgaggaa
     3961 cgggaggaga gctgagtgaa acaggcgccg cttatttatg gcgtaatggc ggaatttgat
     4021 aatccggaaa tgctttgcgc cggcgccgag cgcgcccacg ccgccggcta ccgccggatg
     4081 gatgcataca gcccatttcc gattgaagga ttggccgaag tgatcggctt caaacgtaca
     4141 cagctgccat tgattgtgct gatcggcggt ctcctcggcg gcatcggcgg gttttacctg
     4201 cagtattgga tatcggcaat cgactatccg atgatcattg gcggcagacc gtacaacagc
     4261 tggcccgcct ttattccggt caccttcgaa acgactattc tgggcgccgc cttggccgcg
     4321 gtgctgggca tgctcggatt gaacggattg ccgatgcctt atcatccggt attcaacgtc
     4381 gaacgtttcg cgctggcgag ccgcgaccgt ttttttctct gtatcgaggc ggcggacccg
     4441 aaattcgaac tcgatagcac tgtcgaattt ttgcgcagct taaacccaag ctcgattcac
     4501 gaagtggaac catgaccgag tggaccgaga gacgcgctgc aatgttgatt aaggcgaaac
     4561 tgttcaaaac gtcgctgttg attgcgatca taacaatatt tgccagcgcc ggttgtcgcc
     4621 agcgaatggc agatcagccg taccaacgcc ctttggagcc aagcaatttt ttcgatgacg
     4681 gcatggcgtc acgcccggtg gtgccgggca ccgtcgcgcg ccggggcaac gacggagaca
     4741 agcagccgct caatggtaaa gtcgatggca agctggtcga tacgttccca ttccaggtaa
     4801 ccatggaagt gctggcgcgc ggccaggagc gttatgaaat attttgctca ccatgtcacg
     4861 atcgtctcgg caccggccag ggaatgatcg tccggcgcgg ttttccacgc gcgcgatcgt
     4921 tgcacgagac caggctgcgc gatgccccca tcggccactt tttcgaggtc ataacgcagg
     4981 ggtttgggcc gatgccttcc tatgcaaatc agctcacgga gcatgatcgg tgggctgtca
     5041 tcgcctacat tcgggcgctt cagctcagtc gcaatgtgcg gctcgatcaa ttgccgccgg
     5101 aagatcgcgc caagatgaaa gagacccagt gaacgaccct gtcacaaaca gcgatgggag
     5161 catccaaggc ctggcgcggc tggcttttat cgccggcgcc gccggcgcgg ttatctcggt
     5221 catcggcggc ttgttcgaca gcgcgcaatt ctttcgctcc tatctcgtcg cttatatttt
     5281 ctggctggcg attccgctcg gctctttggc ctttctgatg ctgcattttc tcgtcggcgg
     5341 cggttggggc gtgatgatcc ggcgcctgct ggaatcggca acccgcaccc tacccctttt
     5401 ggcacttttg tttgtgccgc tgctgttcgg actccaagcg gtttaccctt gggccagccc
     5461 ggggcatgtc gccgaggatg aaatcctgcg ccaaaaatcc gcttatctga atgtgccctt
     5521 ctttatcgcc agaaccattt tgtacctcgg tctttgggga gtcctgggtt attttctaaa
     5581 ccgctggtcg cgggagcaag acacatccgc ggaactgccc gccggaaaac gcatgcgccg
     5641 ccttagcggt cccgggttgt tgatctatct tttcacgatg agcttcgcat cggtggattg
     5701 gatcatgtcc atcgaaccgc actggtactc caccatctac ggcttcatct ggatcgttaa
     5761 tcaaggagtc gcggcgatgg cctttgcgat catcgggctg gcgtggctgg gcgcgcgcga
     5821 acctctggca acaccgctgc gcccgggcca ttttcaagat ctcggcaatc tgttgctcgc
     5881 attcatcatg ctttgggcct acatggtgtt ttcgcaatac ctgattatct ggtcgggcaa
     5941 tctggccgag gaaattcctt ggtaccttgc gcgcaccgct gggctctggc gctggctgcc
     6001 gccgctgctg gtgatttttc atttctttgt gccgcttttc ctgcttctaa tgagggacgt
     6061 taagcgcgac cccaatgttt tggtttgggt cgcggcgggc gtactggtga tgcggctttt
     6121 ggacacttac tggatgattg tgccgggctt tgaaaactcc gctctccatt ggcttgacgc
     6181 caccctgctg ctcgggatcg gcggactctg gttgggggtt tttctctggc aactgcaaaa
     6241 gatgccgctg ctgcccctgc atgtagcgct gctggcggag gaagcaaata cccatggctg
     6301 acgcccacca actgaagtat gaaaccgagg atttcacccc gcgtggcgtc cgctggttca
     6361 ccgccgcgct ggcagtgttg gcggtgatgg tcttggcatt gatgggcggt ttgttgtggg
     6421 ttctgagcga tggccggagg ccgggacaag ggatcgtcac tcccggcgta ccggcgccga
     6481 atatgtcgcc ggcgccgaat cttcaggtgg catcttccct cgattatcag caaatgcgag
     6541 ctgcggagga ggcgcaattg catagttacc gatgggtcga tcgagagtcc ggcgttgctg
     6601 cgataccgat tgaacgtgcc atggaaattt tggcggcggg tgggctgccg gcgcaaaagc
     6661 cacaggcaaa aagcccgcaa agcaaacggg agcaaccgtg aagcatgtcg gcagaggatt
     6721 ctcggcggcg atcctggcgt tcgccctgag ccagattgtg gcgccggaaa cggcgtaccc
     6781 gcatgacgaa aaagccgctc gaattgcagc agacaatcaa ccggcgattt ttaaaaatat
     6841 cggcatcgaa caaaagctcg gcaccaagct gccgttaaaa acaatttttc gcgatcaaac
     6901 cggctcctcg atttcgttgc aagactatct atcggcaaag cccgcggtgc tggttttctc
     6961 ctatctcgag tgcccaatgc tctgccctct gctgttggag ggattggtgc gaagcctgaa
     7021 accgttgtcg ctcgacgccg gccgcgattt cgacgtggtc gtgatcagca tcgatgaacg
     7081 agatacgccg gagctggcgc aaaaaaagaa actcgagatc atcaatcgct ataaccggga
     7141 tagcagcggc gccggctggc actttctgac gggagagaaa agcgccattg aagaagtgac
     7201 ccgaagcgcc ggattcagct atgcctggga tgaggcgagc aagcagtacg tgcacgcatc
     7261 cgggattttc attcttacgc ccaagggaga aatcgcccgt gtgctctacg gcatcgatta
     7321 tgcgccgcgc gacgttcgtc tggcactggt ggaagccggc gacggcacca tcggcaccgc
     7381 catcgatcaa ctgctgctct actgctatca ctataacccg ctcaccggaa agtatggatt
     7441 cgtaattatg ggcgctctgc gtctggcggg aatatcgacg gtgctggcga tggcaacctt
     7501 tatcatcacg atgctgcggc gcgagcggcg cggctcacac ggggaagcgg aggcgtagcg
     7561 atgttcgaag acctcccgct gttcccggtc cgagcctcca ccttcgccgc ccaagtcgat
     7621 acgctttact tctttttagt ggcgatggcc acttttttcg tggttctgat attcggcacg
     7681 gttatcttct ttacggcaaa ataccggcgc cgctccgcga ccgagcggcc gccgccaatc
     7741 cacatcgaca tgcgcctgga gattctctgg acgattattc cattgattct ggtcatgatc
     7801 agtttcggtt ggggcgccag cctgttcgtg caaatgtccc aagcgccgga agacgccttg
     7861 gaactgcgcg cggtgggacg acaatggatg tggaagctgc agcatccaag cggtgcccgg
     7921 gaaatcaacc agatgcatgt tccggtgggc aagcc
//
LOCUS       DASUVS010000283         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_987627, whole genome shotgun sequence.
ACCESSION   DASUVS010000283 DASUVS010000000
VERSION     DASUVS010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987627"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1386)
                     /gene="rlmD"
                     /locus_tag="VF182_15590"
     CDS             complement(97..1386)
                     /gene="rlmD"
                     /locus_tag="VF182_15590"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX6438560.1"
                     /translation="MSQTNAKNELTLDIDALSYGPYGIGRHHGKAVMIPKTAPGDGVI
                     ARILQAKERYSIGEIVRILRPSSLRQEPPCPYVAQCGGCPWQHVRYEAQLLAKQKSLE
                     DALRRIGKLEDFEVKPIIASPFTYHYRRRIRLQRDRSKRLGFFSSFSHHVIEIDRCLI
                     ADEKLNGLIERVRQWSEELITDVEHLEVVTGDEPNQNVVVGKSAGGFNSRDSAICERL
                     IKENQYINGLILRGADWRRTWGEVGVSIIPEEGLCVRVDGDVFTQVNSQGNRQLLKEL
                     LAAADFNRDDRVLELYSGAGNFTQSIATRVSDVVAVEGDRPAFESGKRNADLNGITGI
                     QWICAPVAVALTELKKQRRRFSKIVLDPPRTGAKGLDRELAALGAGKILYISCNPTTL
                     ARDLAALSQRGYSLRTVQPIDLFPHTFHVEAIATLVR"
     gene            1479..1850
                     /locus_tag="VF182_15595"
     CDS             1479..1850
                     /locus_tag="VF182_15595"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX6438561.1"
                     /translation="MEAQDDPRLQKGIEEFNQQLFFECHETLEEIWLEERGEDRKFYQ
                     GIIQIAAGYFKLQQGVPAGAIKLWRMGLEKIEPYSPVYLGVELASFSAAVKRNLDELE
                     MVQKTGAASPTLAAPTLCRLS"
     gene            1863..>2233
                     /locus_tag="VF182_15600"
     CDS             1863..>2233
                     /locus_tag="VF182_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010473379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6438562.1"
                     /translation="MIRCLDLRKTYRQGDNEVTVLSGITVDIPQGQFATIMGPSGSGK
                     STLLHLMGGLDRPSGGDVLVGERLISQMADDQVTVFRRTQIGFVFQFFNLLPTLTALE
                     NITLPFVLDGRPKHKADQRAK"
BASE COUNT          475 a          620 c          620 g          518 t
ORIGIN      
        1 ttagcccggc cgcttgaagc ctccctgact gcgcctttca gcagagccgt tcagccccag
       61 cgacaataga tgtcctcgct cgaacttgtc aacggtctat cgcaccagcg tggctatcgc
      121 ctcgacatgg aacgtgtgtg gaaatagatc gataggttga acagtacgca acgagtaacc
      181 ccgttggctc agggcggcaa gatcgcgggc gagggtcgtg gggttacagg agatgtacag
      241 gatttttccg gcaccgagtg ccgccagctc gcggtcgaga cctttggcgc cggtgcgcgg
      301 cggatcgaga acgattttag agaagcgccg gcgctgcttt ttgagttccg tcagagcgac
      361 ggcaaccggc gcgcaaatcc actgaatccc ggtgatgccg ttcaaatcgg cattgcgctt
      421 gccgctctca aaggccgggc gatcgccttc caccgcgacg acgtcgctga cgcgcgtggc
      481 aatggactgt gtgaaatttc cggcgccgct atatagctcg agcacgcggt catcgcggtt
      541 gaaatcggcc gctgccaata attccttgag aagttgccga ttgccttggc tgttgacttg
      601 agtaaatacg tcgccatcga cgcgcacgca caagccctct tcaggaatga tcgaaacccc
      661 cacttctccc caggttcggc gccagtcggc acctcgcaga attaatccgt ttatatattg
      721 attctctttt attaggcgtt cgcaaatcgc gctgtcgcgg gaattgaatc cgcccgctga
      781 ttttccgacg acaacgtttt gattcggttc gtcgccggtg acaacctcca gatgctcgac
      841 atcggttatc aattcctcgc tccactgcct cactcgttca ataagaccat tgagcttctc
      901 atcggcgatc aggcatctgt cgatctcgat cacgtggtgc gaaaacgaac taaaaaagcc
      961 gagccgtttg gatcgatcac gttgcaagcg aatacgtcgg cggtagtgat aggtaaacgg
     1021 cgaagcgatg attggtttga cctcgaagtc ctcgagtttg ccgatgcgcc gcagcgcgtc
     1081 ttcaaggctt ttttgcttgg ccagcagctg ggcctcgtag cgcacatgct gccagggaca
     1141 gccgccgcac tgagctacat aagggcaggg tggctcctga cgcagagatg acggccttag
     1201 tatgcggact atttcgccaa tcgaatatcg ctccttggct tggagaatac gcgcgatgac
     1261 gccatcgccg ggcgcggtct tgggaatcat caccgccttt ccgtgatgcc gcccgattcc
     1321 atacgggccg tatgaaagcg cgtcgatatc tagcgtgagc tcatttttcg cgttagtctg
     1381 cgacatggta actagatttt gaacctttat tgactataca agctgtcgag caagttgcca
     1441 ctctcggcgc tgctcagtcg tatttaggaa cgcctccgat ggaagcacaa gacgatccgc
     1501 gtcttcaaaa gggcattgaa gagttcaacc agcagctctt ctttgagtgt catgaaacgt
     1561 tagaggagat ttggttggaa gagcgcggcg aagaccggaa gttttatcag gggattattc
     1621 agattgccgc aggttacttt aaactacagc aaggcgtccc cgccggagcg atcaagcttt
     1681 ggcgtatggg gctggaaaaa atcgagccct atagcccggt ttacttgggc gttgagctgg
     1741 cctccttcag cgcagccgtc aaacgaaacc tcgatgagtt ggaaatggtt cagaagaccg
     1801 gcgcggcttc tccgactctc gccgctccca cgctttgccg cctcagttga tcctcgcacc
     1861 acatgattcg ctgcctcgac ctgcgcaaaa cctaccgcca gggcgataac gaagttaccg
     1921 tcctatcggg gattactgtc gatattccgc agggtcagtt cgcaacgatc atggggccga
     1981 gcgggtcggg gaagagcacg ctgctgcatc tcatgggtgg cctcgatcgt cccagcggtg
     2041 gtgatgttct ggtcggcgag cggttaatat cgcaaatggc cgatgatcaa gtaacagttt
     2101 tccgccgaac ccagatcggc tttgtcttcc agtttttcaa tttattgccg actctgaccg
     2161 cgttggagaa tatcactttg cctttcgtgc tcgacggccg gcccaaacat aaagccgacc
     2221 aacgagcgaa gtt
//
LOCUS       DASUVS010000284         4048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_987661, whole genome shotgun sequence.
ACCESSION   DASUVS010000284 DASUVS010000000
VERSION     DASUVS010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987661"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VF182_15605"
     CDS             complement(<1..945)
                     /locus_tag="VF182_15605"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6438563.1"
                     /translation="MDANTAKRIIEKNLGYEEVKRLLVKLVQQPSPQTSMLESEPQVL
                     ALIRDVIKPELEHASLRPVIDAMGNLILHIKGRTRTDRLMLVGYAMNAAPSTMPNPYS
                     GEIVDGAPYKLNGECVWGRGACEQKGSLAAMLAAIRFLGAGKIELPSDLYLIVSTAGE
                     TGRHDSLAYVLDHDRVEAEWCIIDGPPEIQLGNKGRIDILVIVRGKQSHSSRPWEGVN
                     AIDGAMKVLEKLKALMPHPEAKTHGSLGKVSLATNAIESFPKATHTIQSECHIMFDRR
                     LLPGDDPKKAVQQMVDAIGRIEPYDIEVQPRDFMYPSEV"
     gene            complement(950..2668)
                     /locus_tag="VF182_15610"
     CDS             complement(950..2668)
                     /locus_tag="VF182_15610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6438564.1"
                     /translation="MLNRIIDSFRREPQIAVVAFVAIVIGFLSLSPTVMLFYGSFLSQ
                     PLGVPGAFTLSNYINAYSDPLTYRLLFNSFLFATGSAVIATVLAATLAWIAIRTNAPF
                     RRFFELTAIVPNIFPPVMLAVAWTVLLSPRTGLINRTLMEIFSLSEAPFNIYSLWGMV
                     FVESLITTPLAFLIVSASLYSMDPSLEESARVAGSSNLQIAWRITFPIVRPALLSAAM
                     LNFVRAIESFDTPAIIALPARIEVFTTKIYREAIGAFPPNQNLAATYAVSLLLITMLF
                     VYFYRGLTKRSERYVTVTGRGYRPTIIDLGKWRYLASAVAGLILIMIVVLPFLVMIYV
                     SFITYVHVPGAKTWELLTLDNYVGNLSDTRTYRALQNSLLLAIGGATLCMLLATLTAW
                     VTTKTKTAGRGVIEALTFIPWAFPGTALAIGLLWTYVYVPLPIYGTLWILLIAYITRF
                     LPYGLRTMTSTIVQLHDDLPQASMVCGAGFLATFRRILLPLLRPGFLAGWIILATIYL
                     REFSTSVFLYSPGAEPLGPLLYHFYVDGNLGPMCALGVIVSVVCVAMIIIARRIGRVG
                     EQFEGR"
     gene            complement(2767..3789)
                     /locus_tag="VF182_15615"
     CDS             complement(2767..3789)
                     /locus_tag="VF182_15615"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6438565.1"
                     /translation="MGSLIESRKVLLMVLWLIAGLTPAIALAQQANVEAAKTEGKVIV
                     YGSVVPQAMDGLHAAFKKKYGIDVEYWRGSSTQVSERALSEWRAGRPGFDVVEGNRGV
                     QLIMKSEGLFQKYVPPSSEKFPAQFKEKDALITPWRVLPISILYNSDMVKANEAPKTL
                     DDLLDPKWSGKISIPDPTRHTTTAQFLWNLQMFKKDKWLDYVKALAKQRPILVDSLAP
                     VTTTIIKGEALVGITYIKYVKQYKGPVDYVLQDKYLTDPNYLSLSAKTSRANAAKLYI
                     DFACSPAGQKEIAEDGEFVMAPGIYPPIKNAEKVAPNMVFMENPSAEEFKKLMGGTFR
                     DIFFAK"
BASE COUNT         1024 a         1090 c         1041 g          893 t
ORIGIN      
        1 gacttcgctc gggtacatga aatccctcgg ctgaacctca atgtcatagg gttcgattct
       61 gccgatggca tcgaccatct gctgtacggc cttcttggga tcgtcgcctg gaagaaggcg
      121 gcgatcaaac atgatgtggc actcgctctg gatagtgtgt gtcgccttag gaaaactttc
      181 aatggcatta gttgcgagac ttactttgcc taaggaaccg tgggtcttgg cctctggatg
      241 cggcatcaac gctttaagtt tttctaacac cttcatcgca ccgtcgatgg cgttaactcc
      301 ctcccatggc cggctactgt ggctctgttt accgcgcaca attaccagta tatcgatgcg
      361 acctttgttg ccgagttgaa tttcgggtgg gccatcgatg atgcaccact ccgcttcgac
      421 tcggtcatga tccaaaacgt aagcaagaga atcgtgccgc ccagtttcac ccgcggtact
      481 aacaatcagg taaagatccg acggaagttc gattttgccc gcgccgagaa agcgaatcgc
      541 cgcgagcatc gccgcaagac tccccttctg ctcacatgct ccccggcccc acacgcattc
      601 tccattcaat ttgtagggcg cgccatcgac aatctcccct gaatagggat ttggcatcgt
      661 gctcggagcc gcgttcatgg cgtacccaac cagcatcagg cggtcggttc gcgttcttcc
      721 tttgatgtgc aggatgagat tacccattgc gtcgatcaca ggacgcaagc tcgcgtgctc
      781 gagctctggc ttgatgacat cgcgaatcaa ggcaagcacc tggggttcgg actccagcat
      841 cgaggtctgc gggctcggct gttgaaccag ttttaccaac aggcgcttga cctcttcgta
      901 gcctaagttt ttctcgataa tcctcttggc cgtgttcgcg tccataacat caacgccctt
      961 cgaactgttc tcctaccctg cctatgcgcc gcgctatgat aatcatcgcg acgcagacga
     1021 cgctaacgat aacacccagc gcgcacatcg ggcctaaatt gccatcgacg taaaagtgat
     1081 aaagcaatgg cccgagcggt tccgcgcccg gtgaataaag aaataccgag gtactgaact
     1141 cacggagata aatcgtcgct agaataatcc aaccggccag aaacccgggg cgcaagagcg
     1201 gcagcaggat gcgccggaag gtggctagaa aaccggcgcc gcaaaccatc gaggcctgcg
     1261 gtaaatcatc gtgcaattgc acaatagtgc tggtcatagt gcgcaggcca tatggcaaaa
     1321 agcgcgtgat ataagcgatc aaaaggatcc acagcgttcc gtagatcggc agcggcacat
     1381 agacgtaagt ccaaagcagt ccgattgcga gcgccgttcc tggaaatgcc catgggataa
     1441 atgtcagcgc ttcgatcacg ccgcgcccag cggtcttcgt tttggtcgtg acccaggcgg
     1501 tcagcgtcgc caggagcata cacaaagttg ccccgccgat ggccaacaac aagctgttct
     1561 gcagggctcg gtaggtcctg gtatcggaga gattgccaac atagttatcc agcgtgagaa
     1621 gttcccaggt ttttgccccc ggtacatgga cataagtgat aaaggaaaca taaatcatca
     1681 ccaggaaggg caaaactacg atcataataa gaatcaaacc ggcaacggcc gaggccagat
     1741 agcgccactt tcccaaatcg atgatggtcg gccggtaacc gcggccagtc accgtcacgt
     1801 aacgttccga gcgcttagtc aatccccggt agaagtaaac aaacaacatg gtgatcagga
     1861 gaagcgagac cgcataggtc gccgccagat tttgattggg cggaaatgct ccgatcgctt
     1921 cgcggtaaat cttcgtcgta aacacctcga tacgagccgg aagcgcgatg attgccgggg
     1981 tgtcgaaact ctcaatcgcc cgcacaaagt tgagcatggc cgccgataat aacgccgggc
     2041 gcacgatggg aaacgtgatg cgccaggcaa tttgtaggtt gctagaacca gcaacgcgag
     2101 cggattcttc gagcgaagga tccatcgaat aaagagaagc cgaaacgatc aagaaagcca
     2161 gaggcgtggt gatcagcgat tcgacgaaaa ccattcccca gagcgaataa atattgaatg
     2221 gtgcctcgga gagactgaag atctccatca atgtccggtt aataagaccg gtacgcgggc
     2281 taagaagcac ggtccacgcc actgccagca tcaccggcgg gaagatgttt ggaacgatcg
     2341 ccgtgagctc aaaaaatcgc cgaaacggcg cgttcgtgcg gatcgcgatc caggccaagg
     2401 tcgccgccag taccgtggcg ataacggctg accccgtagc gaaaagaaaa gaattaaaaa
     2461 gtaaccgata agtaagcgga tcggaataag cgttgatata gttgctgagc gtgaacgcgc
     2521 ccggaactcc gagcggttgg ctgaggaagc taccgtagaa cagcatcact gttggcgaca
     2581 ggctgagaaa cccgataacg atcgccacaa acgccaccac ggcaatttga ggttcgcgac
     2641 gaaatgaatc gatgatccgg ttcaacacct cgacatttcc gttctgtcaa aaatgaagag
     2701 ggccggccct gtaccgggca agccctcctg ttcttctacc tgtccgtctt gagcaaaagt
     2761 tcgcgattat tttgcgaaaa agatatccct gaaagttcct cccatcaatt tcttgaactc
     2821 ctccgccgat ggattctcca tgaacaccat gttgggcgca accttttcgg cgttcttgat
     2881 cggcggataa atacccggag ccatgacgaa ctcaccatcc tcggcaatct ccttctgtcc
     2941 tgccggagag caggcaaaat cgatatagag tttggccgca ttggcgcggc tcgtcttggc
     3001 gctcaggctc aagtaattgg gatccgtcag atatttgtcc tgaagaacat aatcaaccgg
     3061 acctttgtac tgtttcacgt acttgatata cgtaattcca accagcgcct cacctttaat
     3121 gatcgtcgtc gttacgggtg ccagtgaatc gacaaggatc ggcctctgct tagccaaggc
     3181 ttttacgtaa tcgagccatt tatctttctt aaacatctgt aagttccaaa gaaactgcgc
     3241 cgtagtggta tgccgtgtag ggtcgggaat gctgattttg ccggaccatt taggatcgag
     3301 caaatcatcg agcgttttag gcgcctcgtt cgcctttacc atatcgctat tgtacagaat
     3361 gctaattggc agcactcgcc atggcgtaat caaggcatct ttttctttga actgggcagg
     3421 aaacttttca gaggatggcg gcacatactt ttgaaacaaa ccctcgctct tcatgattaa
     3481 ctggacaccg cggttgcctt cgacaacatc aaaccccggc cgaccagcgc gccattccga
     3541 caacgcgcgc tccgaaactt gggtcgaaga gccgcgccag tattcgacat cgataccgta
     3601 tttctttttg aatgccgcat gtaatccgtc catcgcctgg ggcacaacgg aaccatagac
     3661 gatgactttg ccttcggttt tggccgcttc aacgtttgcc tgttgcgcaa gcgcaatcgc
     3721 cggcgtcaaa ccggcaatta gccaaaggac catgagcaaa accttccggc tctcaatcag
     3781 tgatcccatg aagcctcctt aaaatagcga ttaagcacgt attacctata tatcgaaagg
     3841 cactaagtca agaaaacagg ggatttatta tgcctattcg attgcaagca ttcgctcgaa
     3901 atcgcttggt tatgggctgt cacaagggct atttaagcag tgcggaatgt tcgggcgaat
     3961 tagaaatcag atcggttgca catgtggtcg cagatagtgg actgaaaaca gtcgaactct
     4021 ttgggattag gcgacaagcc gcgcggcc
//
LOCUS       DASUVS010000285        11532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1645461, whole genome shotgun sequence.
ACCESSION   DASUVS010000285 DASUVS010000000
VERSION     DASUVS010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11532
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645461"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..668
                     /locus_tag="VF182_15620"
     CDS             33..668
                     /locus_tag="VF182_15620"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX6438566.1"
                     /translation="MSFEIGKNRIDALSDGIFAIVMTLLILEFHVPNLPSNASNVEVA
                     PALVALLPKFASYIVTFVSLGFFWVGHHVMYHAIRRADRTLLWLNIVFFMFVSLLPFS
                     TSLLNAFSEAFIAPLFFGSNLAIIGWILFFQWRYASSQPDMMAAFVSAEYCNTVRSRM
                     LIVPVATTLTVFICFWSAGISLAIYLLLLPLYMLPGKLRGASTAQGFRSAS"
     gene            1077..1814
                     /locus_tag="VF182_15625"
     CDS             1077..1814
                     /locus_tag="VF182_15625"
                     /inference="COORDINATES: protein motif:HMM:NF038123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spondin domain-containing protein"
                     /protein_id="HEX6438567.1"
                     /translation="MVMKLIVISAGLLLSVAGVSAADDGMATKFTVRIENTTKPDAFT
                     ASNGEKWSLAFSPGMAVVHTEKAPIFSAVKKDRGQGLEAQSEDGDPTILAKSLQGGKG
                     IKSVVVFNTPMGGTGPGPITPGAAYEIMISAVRGERLSLTTMMGQSNDWFYAPAESGI
                     ELFNNGKAISGDITSQMVLWDAGTEVDQEPGIGSEQGPRQKAPNTGKAENGVVRSAKD
                     VKYARAYAKTSEVMKVTITPAKSPGSN"
     gene            complement(2047..2256)
                     /locus_tag="VF182_15630"
     CDS             complement(2047..2256)
                     /locus_tag="VF182_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015177794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438568.1"
                     /translation="MKQSKFPPGWDQKRVQRVLKHYEQQTEEEALAEDEAAYEDSTQA
                     IMEVPRELVPSIRELIAKHKRTRRA"
     gene            complement(2253..2540)
                     /locus_tag="VF182_15635"
     CDS             complement(2253..2540)
                     /locus_tag="VF182_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HEX6438569.1"
                     /translation="MNVRFYIDPETEAPHIHKHGVDEEEVVDVLLNPGEDRPGREDSR
                     VVIGKTRAGRYLRVIYVSDPEPESVFVISAYELRGKPLFAYRRRLRKKGRR"
     gene            3699..4205
                     /locus_tag="VF182_15640"
     CDS             3699..4205
                     /locus_tag="VF182_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002203032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HEX6438570.1"
                     /translation="MSKRNLIIGVASVALAIAWYAFRPELLFINKTVNEELPVAQAGS
                     VSMAKETEAMVLAKGDFRGLAHETKGEATVHRLADGKRILRLSDFETSNGPDVHVYLV
                     AAEVAKDNATVKQAGFIDLGSIKGNKGDQNYELPADADLNKYKSVSIWCARFGVNFGA
                     ATLGSQSS"
     gene            4362..5933
                     /locus_tag="VF182_15645"
     CDS             4362..5933
                     /locus_tag="VF182_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015206746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX6438571.1"
                     /translation="MPSQNHYDVVIIGTGAGGGTLAHKLAPSGKRILLLERGGYVPRE
                     KDNWNPRVVNLEGKYQTKEMWHDKDGHELHPHTNYYVGGNTKFYGAALFRLRKEDFGE
                     IRHHGGLSPAWPIRYADMEPYYTEAERLYHVHGNRGEDPAEPPASAPYPFPAISHESR
                     IQQLADDFAQLGLRPFHTPLGVMLNEKVSHASRCIRCNSCDGFPCLVNAKSDAQVCAV
                     DPALEHANVTLRTNAYVERLATSPSGREISKVMIKSNGVREEISADIVVSSCGAINSA
                     ALLLRSANDRHPTGLANRSDVVGRHYMGHVNSVLMALSKCPNPTIFQKSLSVNDFYFG
                     TAGWNYPMGHISFVGKLDGVTLKGGAPALTPGWTLDTMARHSLDFWLTSEDLPDSNNR
                     VTLDSSGGIVLSYKPNNEEGHKRLIVKLKELMQQQTACKIHGRECHQGLFGRNLYVGQ
                     RIPLAGVAHQVGTVRFGNDPRTSALDANCKAHDVDNLYVVDGSVFCSSGAVNPALTIM
                     ANALRVGEHLIQRMK"
     gene            5946..6989
                     /locus_tag="VF182_15650"
     CDS             5946..6989
                     /locus_tag="VF182_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015155606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6438572.1"
                     /translation="MKPIGNRHEATAIRKFLSVLVFVSMLAGMGNKALAQLVKSVDAV
                     GMTVSEIERSVEFFSKVLSFEKVSEIEVAGSEYERLQGVFGARMRIARMRLGDESIDL
                     TEYLTPKGRPIPADSRSNDQWFQHFAIVVSDMDKAYQHLRAHKVQHTSTGPQRIPDWN
                     KAAAGIKAFYFKDPDGHNLELIYFPSGKGNPKWQQTNGRLFLGIDHTAMAVSDTARSL
                     DFYREILGLKLVGESENYGTEQEHLNQVFGSKVRISGLRAQSGPGVEFLDYLTPRDGR
                     PTPHDTRANDLWHWQTAIRVGRADAAAERLNNSRVRFVSPGVATIPDRALGITKGFIV
                     RDPDGHGLQLFER"
     gene            7288..9939
                     /locus_tag="VF182_15655"
     CDS             7288..9939
                     /locus_tag="VF182_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017739788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEX6438573.1"
                     /translation="MTQEELRLQEDRGRKANWKRWGPYLSERQWGTVREDYSPYGAAW
                     DSFSHDQARSRAYRWGEDGLAGISDRHQFICVALALWNGHDPILKERLFGLTGSEGNH
                     GEDVKEYYFYLDSTPTHSYMKYLYKYPQSEFPYARLVEENRRRGKGDPEFELIDTGIF
                     DEDRYFDVFVEYAKGAPDDVLIRIEVINRGPEAAELHLLPTVWFRNTWSWSADVQRPW
                     LRLGEGTEGNIPIELHHAYYGKRWFYCEGNPETLFTENETNNSRVFGANNASLYVKDG
                     INDYIVRGVKEAVNAAGVGTKAAGHYRLNVGPGKSAVVKLRLADKEMVSATSRPFGAS
                     FDFTLAERKKEADEFYASVASPNLSADSRDVMRQALAGMLWSKQFYHYVVKQWLDGDP
                     GNPPPPSERFNGRNHDWPHLYNADVISRPDKWEYPWYAAWDLAFHCIPLALVDSEFAK
                     DQLCLMLREWYMHPNGQIPAYEWAFGDVNPPVHAWAAWRVYKVEKKRQGKGDREFLQR
                     VFHKLLLNFTWWVNRKDTSGMNIFQGGFLGLDNIGVFDRSAPLPTGGYIEQSDGTSWM
                     AMYTLNLLAIALELSKEDRAYEDVASKFWEHFIHIAHAMSHRGQNARGLWNEEDGFFY
                     DVLRLPDGSHFPMKIRSMVGLIPLFAVETLEPELLEQLPGFKRRLEWFIENRPDLTGN
                     VACMRTPGMGERRLLSIANQDQLRQTLKYMLDEREFLSPYGIRSLSQVHRDHPYHLPA
                     NGMDHRVDYEPAESSTGLFGGNSNWRGPIWFPVNFLLIESLQKFHYYLGDSFKVECPT
                     GSGKMMTLWEVAGEISRRLINIFLQGADQRRPVFGGTEKFQNDPHWRGLIPFHEYFHG
                     DNGAGIGASHQTGWTGLVAKLIQQYQG"
     gene            complement(10318..10392)
                     /locus_tag="VF182_15660"
     tRNA            complement(10318..10392)
                     /locus_tag="VF182_15660"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10358..10360),aa:Gly,seq:ccc)
     gene            10571..11515
                     /locus_tag="VF182_15665"
     CDS             10571..11515
                     /locus_tag="VF182_15665"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6438574.1"
                     /translation="MKYRILGRTGLRVSAVGLGTMVHAGHFGPMDDRESMSAIETAMD
                     LGVNFIDTSDAYGAGYSETLLGQALRGKRDRIIIATKGGNVMVGPDRGKRIFTPDYIT
                     RVMDESLKRLQTDYVDLYQLHNPTVDVIERGEVWDVLDRAKKAGKIRHYGVSINSIEE
                     GIAAVNGGRSDTVQLEYNLLAQEPAEKIFPLAQEANIGIIARVPLRRGILTGKLTAAD
                     DGRFQGEDVRARSFKGEPFLRELQKAEQLRFLVDGNRQTFAQAAIAFCLAHAAVSTTI
                     PGARNAQQMRENAAGADVTISPEHLKTIEELWRGCFSS"
BASE COUNT         2679 a         3113 c         3232 g         2508 t
ORIGIN      
        1 cgcgtaggca gaatgaggga gggtgagttc ccgtgagctt cgaaatcggt aaaaaccgaa
       61 ttgacgcgct cagcgatggc atatttgcca ttgtcatgac ccttcttatc ctcgagtttc
      121 acgtccccaa tctgccgtcc aacgcatcca atgtcgaggt cgcgccggcg ctggttgctt
      181 tgttgcctaa gttcgcgagc tacatcgtca cctttgtcag cctgggattc ttctgggtcg
      241 gtcatcatgt catgtatcat gctatccggc gcgccgaccg gacgcttctc tggctcaaca
      301 tcgtcttctt tatgttcgtc tccctgctcc ctttctcaac cagcctgctg aatgcctttt
      361 cagaggcttt cattgcgccc ctcttcttcg gaagcaacct ggcgattatc ggttggatac
      421 tgttctttca atggcgctat gcaagctcac aacccgacat gatggcagct tttgtcagtg
      481 ccgaatactg caacacggta cgatctcgca tgctgatagt tccggttgcg acaacgctga
      541 ccgtattcat atgtttttgg agcgcgggga tcagcctggc gatctatctc ctgctcctac
      601 ctctgtacat gcttccgggg aaattacgag gcgctagcac tgctcaaggt ttcagatcgg
      661 cttcgtagaa gaccggattc ttatcagggg cgaaaacgag ggatcggcgc tctcgggtga
      721 atgttcaatc atcctctgga agataacggt gaaacatctt aaatttgtcc tcgggctttt
      781 tagtattctt tttccgatcg cagcatctcc ggcgctctct acagtccaca ggacgctctc
      841 cagatcatcg aggttgtcgc cctcaatcct cgcttcgata agctcgtgcc gttgaatacc
      901 aagatcgaga agatcgcggg cggccacaat gggtggaagg acccgtgtgg gaccgcgaag
      961 aaggatacct gctcttctcc atcgcatcct ttgccggcac gtccgcatct aagctcgtac
     1021 gcagcgcttc gattgagtca ggcgaagatc aacaaaaact cacaaggagg gaaatcatgg
     1081 ttatgaagtt aatagttata tctgcgggct tgttattgag cgtggccgga gtttcagcgg
     1141 cagatgacgg catggcgacg aaatttaccg tacggatcga gaacactacc aagcccgacg
     1201 cgttcacggc atcgaacggc gaaaagtggt cgcttgcatt ctcgcccggc atggccgtgg
     1261 tgcataccga gaaggcgccg atctttagcg ccgtgaaaaa ggaccgtggc caagggctcg
     1321 aggcacagtc agaagacgga gatcccacta tcctcgccaa gtcgctgcag ggcggcaagg
     1381 gaataaaatc agtggtagtt ttcaacacgc ctatgggcgg gaccggcccg ggcccgatta
     1441 ctccaggcgc agcttacgaa atcatgattt ctgcagtgcg cggcgaacga ttatcgctga
     1501 cgacgatgat gggccagtcc aatgactggt tctacgctcc ggccgaatcc ggcatcgaac
     1561 tattcaacaa cggcaaggcc atcagcggag atatcacatc gcaaatggtt ctttgggatg
     1621 ccggcacgga agtcgatcag gaacccggca tcggctccga gcaaggaccg cggcagaaag
     1681 cgcccaatac cggcaaagcc gagaacggcg tcgtgcgcag tgcgaaggat gtgaaatacg
     1741 cgagagccta tgcgaaaact tccgaggtta tgaaagtgac gatcacgccg gcaaagagtc
     1801 cgggttccaa ttaggcgccc gcgcgacatc ggaagtaaat ccgccggcac gccgcagggc
     1861 ggtcggcgga tcaaagctgc atgatgacaa ggtcgagcta tgaaccaatg tatacacaag
     1921 ccaaaaaacc ttgaggaagg attcggagaa ccgattggcc cttatattga gtaacgcgat
     1981 gccactgagc cgctaaagcc aaattttatt tacgaccaat gttcgccttc ggctcaagcg
     2041 gcggggttag gccctccgtg tccgtttgtg cttcgcgatg agttcccgga tggatgggac
     2101 cagctcgcgc gggacctcca tgattgcttg agtcgaatcc tcataggcgg cctcgtcctc
     2161 agcaagggcc tcttcttccg tctgctgttc gtaatgcttg agtactcgct gcactctttt
     2221 ttgatcccaa cctggcggga atttactctg tttcatctcc ttcctttctt acgtaagcgt
     2281 ctgcgatatg cgaacaatgg ctttccgcgc aactcatacg cgctaatcac aaatacgctc
     2341 tcgggttcgg gatcggaaac ataaataaca cgcaggtagc ggcctgctcg tgttttgccg
     2401 atcacgaccc gtgagtcttc ccgacccggc cgatcctcgc ccggattcaa cagcacatca
     2461 acaacttctt cctcatcgac accatgctta tgaatatgag gggcttcggt ttccggatcg
     2521 atatagaacc gaacattcat ctacgaagga ttatatcact ccacctctgt gtaggcctaa
     2581 cgtccacgct caccggcgcg cgctcgcggt gcctgaaaaa gcgatgcgct agtgagcgcg
     2641 tccgtgtgcg gcgtgtcgtt aggtagcatc ataagcttgg aagcaaagtc agcaatgcga
     2701 aaacctggtg agttgctaag tctcgcgagc tttgaacaat gtcgcgaaca ccaaccctcg
     2761 cggcgccaat agtgagttcc aaacgctgtg gaggtccgcc ggcggcaaga gtgaaatgac
     2821 gtctcatacg agaatgtgtc agccagttac gagctgcttt tatatcggca taccgttgat
     2881 ccgcgaacac accatctacc cactggcgcg cagtagccgg tagtgcggtc cgcaatgtat
     2941 ttgaccggct ggacgaacta ctcggatcga agtctggcat tgcaagctca cgggttgaag
     3001 tatgatcgca aagagcgcga cctaagccag cggcacagag atctagagcg gtgattgagg
     3061 tgcccgttgc ccatctggcg tgcgagacgt ctgcgatatc cgggttatga ccaccaatta
     3121 cggagtggga gacgtcgtat tcgtaatcaa tgtcatccac atagtgtaga cagtcaacgc
     3181 accacgcagc ccaaagtaat agggcacccg cgtccgggtt tgcatgtcgt gcttcgaagg
     3241 gttgcactaa ctgaactgga tcagtaggcc agttatgtct cggcatcgcg ggagtgatgc
     3301 tgtctaacca ttgagtatac tgaccactta tattgctttt actgaaatta ctgcaacgaa
     3361 atttactttt atactcaaga agttgtcaat tatcgtttcg gaggtataac caatattctg
     3421 gtcagtatag catcgatata cctgcacggc ggaatgtggt tcaccagcca tcagctggac
     3481 tacgaacaca tttgccggct ctgttcgata cgtgggtgta cgccaccgtt aagcggctcg
     3541 aactccactg ccggcaagct gttctccgtt ggtaagacta tgcggctctg aacaagatta
     3601 cgggtctgcc aagtctgcat cgtttcactc cttcccgaca tctaacccac agaatttccc
     3661 ctgtcatagt cataaacaaa attaacggag gataagtcat gagtaaacgt aatcttatta
     3721 tcggggtcgc tagcgtcgcg ctggcaatcg cttggtatgc gttccggccc gagttgctct
     3781 tcatcaacaa aaccgtcaac gaggagctgc cggttgctca ggcaggctca gtatcgatgg
     3841 ccaaagaaac agaggcaatg gttttggcga agggcgattt tcgcgggctt gcgcacgaga
     3901 ccaaaggcga agcgacggtt catagattgg cggacggcaa gcgcatactg cggctgagcg
     3961 attttgaaac ttccaatggt ccggatgtcc atgtctatct ggttgccgcc gaagtcgcca
     4021 aagacaacgc cacggtaaag caggccggct tcatcgatct tggttcgatc aaaggcaaca
     4081 agggtgatca gaactatgag ctgccggcgg atgccgatct caacaaatac aaatcggtca
     4141 gcatctggtg cgcgcgtttc ggggttaact tcggcgccgc tactctgggt tcacagagca
     4201 gctagaggac cagtattcct tgtcggctgc tttccctgct ggggaagcag ccggtttgag
     4261 aaagtacgaa aggatttgag atggccaggg gcagaaaaca gtttgagctg tccgcccgtg
     4321 cggttaaaca acaatcccct gcctaagaaa ggaatgagaa gatgcccagc caaaaccatt
     4381 acgatgttgt cattatcggc actggcgcag gcggcggcac tctggcgcat aaacttgcgc
     4441 ccagcggcaa gcgcatcctg ctcctggaac ggggcgggta tgtgccgcgc gagaaagaca
     4501 actggaatcc ccgcgtcgtc aacctcgaag gaaaatatca gaccaaagag atgtggcatg
     4561 acaaagacgg ccacgaactt catccgcaca ccaactatta cgtcggcggt aataccaaat
     4621 tctacggcgc ggcgctgttc cgtctgcgca aagaagattt tggtgaaatt cgtcatcacg
     4681 gcggcctctc tccggcttgg cccattcgtt acgccgacat ggagccttat tacactgaag
     4741 cggaacggct ttatcacgtg cacggcaacc gcggtgaaga tcccgccgag ccgcccgcca
     4801 gtgcgcccta tccatttccg gcgatcagcc acgaatcgcg tattcaacaa ttagcggacg
     4861 atttcgcgca gctcggcctg cgcccgtttc acacgccgct tggcgtaatg ctcaacgaaa
     4921 aggtttcgca tgcgagccgc tgcattcgct gcaacagctg tgacggattc ccttgcctgg
     4981 ttaacgccaa gtccgacgcg caggtctgcg ccgtcgatcc ggcgctcgaa catgccaacg
     5041 taactctcag gaccaacgct tacgtcgaac ggctcgcaac cagcccatcg ggccgcgaga
     5101 tcagcaaggt aatgatcaag agcaacggcg ttcgcgagga aatttcggca gatatcgtgg
     5161 tgtcgtcttg cggcgcgatc aattcggcgg ccttgctgct ccgctccgcc aatgaccggc
     5221 atccgaccgg tctggcgaat cgttccgatg ttgtcggccg acactacatg gggcacgtca
     5281 actcggtcct gatggcttta tcgaaatgcc ctaacccgac gatctttcag aaatcgcttt
     5341 cggtgaacga tttctatttc ggcacggccg gctggaatta tccgatgggc catatctcgt
     5401 ttgtcggcaa gctcgatggc gtgacattga aagggggcgc gcctgcgctc acgccaggct
     5461 ggactctcga tacgatggcg cgtcattcgc tcgacttctg gctcacgtcc gaggatttgc
     5521 ccgattccaa caaccgggtc acgctcgatt cctccggcgg tattgttctc agttacaagc
     5581 cgaacaacga agaaggccac aagcggctca tcgtcaagct caaagaactc atgcaacagc
     5641 agacggcgtg caagatccac ggccgcgaat gtcatcaggg actgttcggc cgcaacctct
     5701 acgtcggtca gcgcattccg ttggcaggcg tggcgcatca ggtcggaacg gttcggttcg
     5761 gcaacgatcc aagaaccagc gcgctggacg ccaattgcaa agcccacgat gtggataatc
     5821 tctatgtcgt cgatggcagc gttttctgtt ccagcggcgc ggtgaacccg gctctcacca
     5881 tcatggccaa tgccctgaga gtgggagagc atttgattca aaggatgaag taggaacgcg
     5941 gaaggatgaa gccaataggc aacaggcacg aggcaacggc tatcagaaaa tttctgagcg
     6001 tgctcgtgtt cgtaagcatg ctcgcgggca tggggaacaa ggcgcttgcg cagctcgtca
     6061 agtccgtcga tgcggtgggc atgacggtgt cggagatcga gcggtcggtg gaattttttt
     6121 cgaaagtgct gtcgtttgaa aaagtctcgg aaatcgaagt cgcaggcagc gagtacgagc
     6181 ggcttcaggg agtgttcggg gcgaggatga ggattgcgcg catgcggctc ggcgatgaat
     6241 caatcgatct caccgagtat ctgacgccca aaggcaggcc gatcccggcg gactcgcgca
     6301 gcaacgatca atggttccag cacttcgcca tcgtcgtcag cgacatggac aaagcctacc
     6361 agcatctgcg cgctcataaa gtgcagcata cctccaccgg tccgcagcga attcccgact
     6421 ggaacaaggc cgccgccggg atcaaggctt tttacttcaa ggatcccgac ggtcacaatc
     6481 tcgagctgat ttattttcca tccggtaaag gcaatccaaa atggcagcaa accaacggcc
     6541 gtcttttctt aggtatcgac cataccgcaa tggcagtttc ggatacggcc cggagcttag
     6601 atttttaccg agaaattctc ggactgaagc ttgtcggcga gagtgaaaac tacggcaccg
     6661 aacaggagca tctgaatcag gtattcggat ccaaggtgcg tatcagcggg ctcagggcgc
     6721 agtccggtcc cggagtcgag ttcctagatt atctgacgcc gcgcgatggc cgcccgacgc
     6781 cgcacgatac gcgggcaaat gacctctggc attggcagac agcgataagg gtgggccgcg
     6841 ccgatgcagc ggcggagagg ctaaataatt cgcgcgttcg ctttgtttcc ccgggcgtgg
     6901 cgactatccc ggaccgagcc ctgggaatca ctaaaggatt tatagtgcgc gatcctgacg
     6961 gccacggcct tcagttgttc gagagataat ttgagcacgg caacagaagg tttatcatcc
     7021 tcgttcatgt ctccgagata tcgatgtcat gcgccgcaga caccacgcgg ttgccagtca
     7081 agcctggtcc ctggcagaac tgattcgccc ttgagcgaat ggttctgaga ctcgctgaaa
     7141 aaccggaaat ccgcgcatcc tctccagcgc aagcgggatc taacacgtaa gcatcgcagc
     7201 atcgcgagat catacgactc tcgagaccga tctggcagac aaccggcggt tcttttcgcc
     7261 gctgatcacc gcaaacagga gagcatcgtg acacaagaag agctcagatt gcaggaagat
     7321 cgaggccgga aggcgaactg gaaacgttgg gggccttatc tctcggagcg acaatggggc
     7381 accgtgcgcg aagattacag tccatacgga gccgcatggg attctttttc tcacgatcag
     7441 gcgcgtagcc gcgcctaccg ctggggcgaa gacggcctgg cagggatttc ggatcggcac
     7501 cagttcattt gcgtcgcttt ggccctgtgg aacggccacg atccgatcct caaagaacgg
     7561 ctcttcggcc ttaccggcag cgagggtaac cacggcgagg acgtcaaaga atattatttt
     7621 tacctggact cgacaccgac gcactcttac atgaaatatc tgtacaaata tccgcagagt
     7681 gaatttccct atgcgcgtct cgtggaggag aatcgacggc gggggaaagg cgacccggaa
     7741 ttcgagctca tagataccgg aatttttgac gaggaccgtt acttcgatgt gttcgtcgaa
     7801 tacgcgaagg gtgcgccgga cgatgtgctc attaggatcg aagtaatcaa tcgcggaccc
     7861 gaagccgctg agcttcacct cttgccgacg gtttggtttc gcaatacctg gtcctggagc
     7921 gccgatgtgc agcggccttg gttgaggcta ggtgagggca ccgaaggcaa cattccgata
     7981 gaactccatc acgcatatta cggcaaacga tggttttact gtgaagggaa cccggaaacg
     8041 cttttcaccg agaatgagac taacaatagc cgtgtcttcg gcgcgaacaa cgcttcattg
     8101 tacgtcaagg atggaatcaa cgattacatc gtgcgcggcg tcaaggaagc ggtgaatgcc
     8161 gcaggcgtcg gaaccaaagc ggccggccac tatcggctca atgtcgggcc gggaaagagc
     8221 gccgtggtaa agcttcggct cgcggacaaa gaaatggtct ctgccacgag ccggccgttc
     8281 ggcgccagct tcgatttcac cttggcggag cggaaaaaag aagcggatga attttatgcc
     8341 agcgtcgcgt ctccgaatct ttcagcggat tcacgcgacg tgatgcgcca ggcgctcgcc
     8401 ggcatgctct ggtcgaagca gttctaccat tatgtagtca agcaatggtt agacggcgat
     8461 cccggcaatc ctccgccgcc atcggagcgg ttcaacggcc gtaatcatga ttggccccat
     8521 ctgtacaatg ccgacgtgat ttccaggccc gacaagtggg agtacccgtg gtatgcagcc
     8581 tgggacctgg cgtttcattg cattccgctt gcattggtcg attcagagtt cgccaaggac
     8641 caactctgtc tcatgctacg ggaatggtac atgcacccga acggccagat tccggcatac
     8701 gagtgggcct tcggtgacgt taacccgccg gttcacgcct gggcggcgtg gcgcgtctac
     8761 aaggtcgaaa agaagcgcca aggcaaagga gatcgcgagt ttctccagcg cgtgtttcac
     8821 aaactgctgc tgaacttcac ctggtgggta aaccgcaagg acaccagcgg catgaacatt
     8881 ttccaaggag gatttttggg cctggataac atcggcgtgt tcgatcgcag cgcgccgctg
     8941 cccactggcg gctacatcga acagtctgac ggcacgagct ggatggcgat gtacactttg
     9001 aatcttctcg ccattgcact cgagctatcc aaggaagacc gcgcctacga agacgtggcg
     9061 agcaaattct gggagcactt catccacatt gcgcatgcca tgagccatcg cggtcagaac
     9121 gccagggggc tgtggaacga ggaagacggc ttcttctatg acgtgctcag gctgccggac
     9181 ggcagtcatt ttccgatgaa gatccgttcg atggtcgggc tgattccgtt gttcgccgtg
     9241 gaaaccttgg aaccggagct gctggagcag ttgccgggtt tcaagcggcg gctggagtgg
     9301 ttcatagaaa atcgcccgga cttgaccggc aacgttgcct gcatgaggac gccaggaatg
     9361 ggtgagcgcc gcttgctttc cattgccaac caggaccagc tccgacaaac gctcaagtat
     9421 atgctcgacg agcgcgagtt cctttcgccc tacggaattc gctcgctatc tcaggttcat
     9481 cgtgatcatc cgtatcattt gccagcgaac ggcatggacc atcgggtgga ctatgaaccg
     9541 gccgaatcca gcaccggact tttcggcggc aattccaatt ggcgcggtcc gatctggttt
     9601 ccggtgaact tcctgctgat cgaatcgttg cagaagtttc attactatct tggagacagt
     9661 ttcaaggtgg agtgccccac cggttccggc aagatgatga cgctctggga agtcgccggc
     9721 gaaatttcgc gccggttgat caacatcttc ttgcagggtg cagatcagag aagaccagtt
     9781 ttcggcggca cggaaaaatt ccagaacgac ccgcactggc gcggtctgat tccattccac
     9841 gaatacttcc acggcgacaa cggagcgggc atcggcgcga gccatcaaac cggttggacc
     9901 ggcctcgtcg ctaagctcat ccagcaatac caggggtgag taccggcgac ggtagtaaat
     9961 caagtgaaca atcaaacgga gatggcgacg atcgcttaac aataacgatc ttcagtcatt
    10021 ggccgaaaca gcatttacga ggcttgtgat cttcttgaga tcggaacaga gtgacggttt
    10081 tctgctatcg ccacaaaact cggaatgttc ctaagatctt atcttacgaa agatcttcaa
    10141 atcgatctcc gacgaatgag tgtcaaccgt gtgccttgac gaaacacact gcagttttca
    10201 atcagaagct gagcaccggg aaaattatct ttaagaatct ttcttgctca ttaaaacccg
    10261 agatttgccc gaaataaaaa aagctcatac ggagaacgtg taagcctttg aaaagtttgg
    10321 agcgggcgac cggggtcgaa ccggcgacgt ccagcttggg aagctggcat tctaccgctg
    10381 aattacgccc gctcatcagg gtggatgtgg agttaaaata tggctttacc cggagcttgt
    10441 caacgagagt cactcgccgc gcttgcagta tttgtcgcgg tcgcagggct gtcggattgt
    10501 cgaacatcgt tcgtgatctt gcgtatgaac gccgacgtgt tttatgttgg cggcagtaac
    10561 cggaggataa atgaagtacc gaattcttgg cagaacaggt ttgcgggtga gcgctgttgg
    10621 tctgggtacg atggttcacg ccggccattt cggtccaatg gatgaccgtg aatccatgag
    10681 cgctatcgaa actgcgatgg acctgggagt gaacttcatc gacacctcag atgcctatgg
    10741 cgcaggctat tcggaaacct tgctcgggca agccctgcgc ggcaaacgtg accggataat
    10801 tatcgccacc aagggcggga acgtcatggt cggtcccgac cgcggcaagc gaattttcac
    10861 gcctgattat atcactcgcg tcatggacga aagcttgaag cggctccaaa ccgactatgt
    10921 cgatctctat cagctgcaca accccacggt cgatgtaatc gagcggggcg aagtgtggga
    10981 cgtgctcgat cgggctaaaa aagccggcaa gatccggcat tatggagtct ccatcaatag
    11041 tatcgaggaa ggcatcgcgg cggtaaacgg cggccgctcg gatacggttc agctcgagta
    11101 taaccttcta gcgcaagaac cagcggaaaa aatatttcct ctggcgcaag aagccaatat
    11161 cggcattatc gcgcgggtgc cgctgcgccg cggaattttg accggaaaat taaccgcggc
    11221 tgatgatggg agatttcagg gtgaagatgt gcgggcgcgc agcttcaagg gcgagccgtt
    11281 tctccgggag ctccaaaaag cggagcaact ccgatttctg gtagatggaa acagacaaac
    11341 ctttgcccaa gcggcgatcg ctttttgtct cgcccacgct gcggtcagca cgacgattcc
    11401 gggcgctcgc aatgcccagc agatgcgtga aaatgccgca ggcgcggatg tgacaatctc
    11461 tcccgagcac ttaaaaacga tcgaggagtt atggcgcggc tgtttcagtt cgtgaccgct
    11521 gatggttcgg ct
//
LOCUS       DASUVS010000286        18098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1646401, whole genome shotgun sequence.
ACCESSION   DASUVS010000286 DASUVS010000000
VERSION     DASUVS010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 18098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18098
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646401"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..2801
                     /gene="acnA"
                     /locus_tag="VF182_15670"
     CDS             108..2801
                     /gene="acnA"
                     /locus_tag="VF182_15670"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX6438575.1"
                     /translation="MAQKYVDSFGARKVLKVGDQTYDIFRLDMLENAGFKNISRLPVS
                     LKVLLENLLRQEDNHHVTKADIEALANWNPKVKSEKEIAFMPARVLMQDLTGVPAVVD
                     LAAMREAMKRLGGDPKKINPLAPVDLVIDHSVQVDFFGTADALHKNSEIEFKRNVERY
                     AFLRWGQTAFDNFRLIPPDTGICHQVNLEYLAPVVFRAQKDGAHFAYPDTVVGTDSHT
                     TMINGLGVVGWGVGGIEAEAALLGQPIIMLIPEVIGFKLHGKLPEGATATDLVLTVVQ
                     MLRKKGVVEKFVEFYGAGLSSLSLADRATIGNMGPEYGATIGYFPIDDETLRYLELTG
                     RDPHLIELVESYAREQGMFRTDSSSDPMFTDSLELDLATVVPSLSGPRRPQDRIPLTE
                     SKKSFKEALPSLMKSGDANKTVTVQNNGDSFQLTHGSVVISAITSCTNTSNPSVMIGA
                     GLLAKKAAEKGLASKPWVKTSLAPGSKVVTEYLKDSGLLPYLEDIGFHLVGYGCTTCI
                     GNSGPLPEPVVNAIHEGDLVAAAVLSGNRNFEGRIHPNVRANYLASPPLVVAYALAGH
                     MDTDLYNEPLGTDSDGKAVFLRDIWPTPEEIQEVMRKSVRSEMFRTEYSRATEGDELW
                     QNMPVPRGELFAWDPQSTYVREAPYFDGMSRTPAELKEIKGARVLALVGDSVTTDHIS
                     PAGSIERNGPAARYLTNNDVLPKDFNQYGARRGNHEVMMRGTFANIRLKNMLAPGTEG
                     GVTVHLPDKKPMSIYDAAMQYQKEGVPLMVVAGKEYGSGSSRDWAAKGPRLLGVKAVI
                     AESYERIHRSNLIGMGIVPLQFKPGENLKAHNLTGFETFEIVGISAGLKLRQTLAVRA
                     TKDDGEIQEFTVICRIDTPAELEYYRHGGILEFVLRQLLQS"
     gene            2874..3788
                     /locus_tag="VF182_15675"
     CDS             2874..3788
                     /locus_tag="VF182_15675"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6438576.1"
                     /translation="MNRRQALALIGPGSLALLLGNSRLFRKHPYAAERPIMNQPAHIA
                     HFTADNPGMMTLQGTNQYIVGKDSAVVIDVALSSDGNMDGIIEQGEAMGVKKIEKILL
                     THIHIDHCGGALALKKRTGAKLGIHRSRAGYLGGEDFQYDDQDRIAFGGGELQVLHTP
                     GHESGHCCFYESGDKVLFSGDNILGYGTAVIHPPDGNMTDYLQSLERLLEFNIDLILP
                     GHGPLIGKPEAKIREYLRHRMMREKQVIEAMRNGRNTIGDITQSIYVDVSPALQRVAE
                     FSVQAHLEKLIRDGRVKREDGRYQMTTD"
     gene            complement(3936..4676)
                     /locus_tag="VF182_15680"
     CDS             complement(3936..4676)
                     /locus_tag="VF182_15680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX6438577.1"
                     /translation="MTRLQCAWYQWSSSDISGPSSAVGNVKPISDLSLGLLLVTVISI
                     TACSVPPSRIPPPITPPRASQTGIASWYGPGFHGKATASGVIYNQHDLTAAHQTLPLG
                     TRVLVTNLENGRSVEVLINDRGPFAKGRIIDLSYAAAESLGMVGPGTIPVRIEVVGSG
                     ADKVSLIKDSLDYTLQLGSFSVLENARQLRDLVARQFSEVAIVPLNTSSTTYYRVQLG
                     KFSTRSSAEEQARRVTQAGYPVVILEKW"
     gene            complement(4697..5263)
                     /locus_tag="VF182_15685"
     CDS             complement(4697..5263)
                     /locus_tag="VF182_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX6438578.1"
                     /translation="MSFYTNEVAAFAAFFVYGSFFEWTLHKYLMHQPRWKYPFRAHAL
                     VHHGLFRTGADYFLSDKKYRRKVRFAWWNAPFIIALHVPAILLIQDLLGAPILLGACA
                     AMAVYYGLYEYLHYCMHIPTGRRIEQTKWFRWLDAHHHMHHKRHLNNLNVVLPLADAV
                     FGSLISAKERIPAPDRKQGAYRMEALLG"
     gene            5540..6286
                     /locus_tag="VF182_15690"
     CDS             5540..6286
                     /locus_tag="VF182_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX6438579.1"
                     /translation="MSGHSKWSTIKHKKAAKDAKKGKLFTKLIKEITVAARMGGGDIN
                     ANPRLRTAVFTARSASMPTENIDRAVKKGTGELEGVTYDEIQYEGYGPGGAAILAHVL
                     TDNKNRTVSEIRRLFTKHGGNMGEAGCVAWMFDKKGLIAIEKTQIEEDRLMSIVLDAG
                     AEDVRDEDEIYEVITTVEEFDKVKERLEVEKVSVASAQITMVPKNTVDVDEKNVEQIL
                     KLTEELEDHDDVQSVSANFNIPSELMEKAS"
     gene            6283..6870
                     /gene="ruvC"
                     /locus_tag="VF182_15695"
     CDS             6283..6870
                     /gene="ruvC"
                     /locus_tag="VF182_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX6438580.1"
                     /translation="MRWPAPVRSRDDAAPLARRVLGIDPGSVITGWGVVEAAGNSLIH
                     LAHGTIATGGAQGQAARLERIYRGLEEVIGSNNPDGVCVERVFFARNAQSALKLGQAR
                     GVAILAAARNSLAVHEYAAVEIKAAVVGYGHATKQQVQMMIASLLRLNGKLPADAADA
                     LAAAICHLHMQTLRAHLVEALPKDIAPLPRKSILP"
     gene            6913..7506
                     /gene="ruvA"
                     /locus_tag="VF182_15700"
     CDS             6913..7506
                     /gene="ruvA"
                     /locus_tag="VF182_15700"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX6438581.1"
                     /translation="MIAQISGTLAQKLAGEIVVDVGGVGYQVFIPLNVFYHLPEIGAP
                     VRLHIHTHLREDALQLFGFQEPAEKQVFLQLNGVAGIGPKLALNILSGIPAEELTRAI
                     KEGDQLRLVSIPGVGKKLAERMIVELRDRFAFLESQPAEIPEQGRDPRLIQDAVSALV
                     NLGYRKGEVEKNVRDCVQAGNHSLEQVIKEVLRRMSR"
     gene            7515..8585
                     /gene="ruvB"
                     /locus_tag="VF182_15705"
     CDS             7515..8585
                     /gene="ruvB"
                     /locus_tag="VF182_15705"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX6438582.1"
                     /translation="MSYPPIVNPGNTESREISPARSDEDLNYEVNLRPRGFEEYVGQD
                     RVKENLKVAIDAARGRREVLDHLLFHGPPGLGKTSLAYIIAREMGVNIKGTSGPVIER
                     AGDLAAILTNLEEGDILFIDEIHRLNHIVEEVLYPAMEDFQIDIMIGQGPAARSIKLE
                     LKPFTLIGATTRSGLLTSPLRDRFGLSFRLDFYSPETLTDLLQRSAVILGVGGEQEGI
                     AEIARRSRGTPRIANRLLRRVRDYAQVKAAGRITLAVTVEALQMLEVDSIGLDKMDTM
                     LVLTLIEKFSGGPVGVETLAAAIGEEKDTIEDVYEPFLIQAGFIQRTPRGRMATPLAY
                     DHFGRKSKGQKGTAPKNQPTLF"
     gene            8633..8845
                     /locus_tag="VF182_15710"
     CDS             8633..8845
                     /locus_tag="VF182_15710"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEX6438583.1"
                     /translation="MPEFGRTLIILGIVIIALGVLFTFGGKIPWLGHLPGDIHVQRGR
                     FSFYFPISTCIVISVLISLVLYLFRR"
     gene            8882..11116
                     /locus_tag="VF182_15715"
     CDS             8882..11116
                     /locus_tag="VF182_15715"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6438584.1"
                     /translation="MSKIWDRQPVISDTPRIDEAKRRKREGLAILITSLVVLAFAFFE
                     VQLPDVSSEYSLSNNIVFFLLININIILLILLVFLVVRNLVKLVFERKRRILGSRLRA
                     RLVIAFVGLSLIPTLLLFVIAGGFVTRSFERWFDIQVETALQGSLEIGQTYYQNSANN
                     ALFYARQLSRRVTEQGLFDSQRRSDLKEFIQSKQREYNLGTVELFSPDHLPVVVAFND
                     QVPTGVSIKAEPDFLNRALRGLEVTRTQAFGDGDIIRGGVPIYDSGKRIQGVVVVDYY
                     VPKSITKRALEISRSYEQYKHLGFLKNPVKNSYILTLLLVTLVIIFAATWFGLYLAKG
                     ITIPIQQVAAGTHEVAHGNWDYQIEAAGDDELGTLVESFNQMTRDLKKSNLELLRRGK
                     FVETLLANITAGVIAVDPTGKITSWNKAAEQMLDVDAATALGKNYGEAFESEPLTVMR
                     ALLDNLNDRESSATELQIQLPDHLLTVFATAATLRDDSNDSLGIMIFLEDITQIQKVQ
                     RMEAWREVARRIAHEIKNPLTPIQLSAERLRKRYAKLLQGDGAVLDKCTTTIIQQVVE
                     LKNLVNEFSQFARLPTAQPVPNDLNGIVQDALFLFKEGHNHIDFDFRQGELPALELDR
                     EQIKRVMINLLDNAVAAVGEGGHVRISTSYDHARNTVYLEVADDGCGMPPEARVRIFE
                     RGFSTKKQGTGLGLTIVNQIIEDHRGYIRVRNNEPRGTRFVIEFPVRQQDALHELKRK
                     AVQL"
     gene            11131..12435
                     /gene="lpxC"
                     /locus_tag="VF182_15720"
     CDS             11131..12435
                     /gene="lpxC"
                     /locus_tag="VF182_15720"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX6438585.1"
                     /translation="MSDLSSQKTILIVDDEDKVRESVKEVLTDEGYRVLDTADATCAL
                     EMIEAEQPGLVLLDIWMPKMDGIGLLKEIKSKKPATNVVMISGHGNIHTAVTATKFGA
                     FDFIEKPVSLDGLLHTVQRALGELPVAQSATEMGQSHGAEDIAESVSAARRSSACAEP
                     IRQKTLRTSVVSSGQGLHSGIKTGLILHPLPPNSGILFEGISGDVSVPAHLDYVGSTG
                     FATSLSHRGFTVATVEHFLAVLHSYGITNLLVKVQGEVPILDGSAAEFCELIEEAGLH
                     EQEESWSRIVIDRPYRFGEDAGEWISIEPAEKFSVHYVLNYPKPVGMQEYLFTHQGPN
                     SFKEEIAPARTFGFLRDMEKMQKLGLINGGKLSNCILIDDEKIVNTDLRFPEELARHK
                     ILDIIGDFYLLGRPIRGKITAHMTGHSDNIGLLKAVRAGMQL"
     gene            12456..12905
                     /locus_tag="VF182_15725"
     CDS             12456..12905
                     /locus_tag="VF182_15725"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEX6438586.1"
                     /translation="MDSSSPYSRLFTINEASALLPVVRPTIEKILEIIRRLRAKSEIV
                     IRAEQIDPETPNLLRRLQQNEEIAALIKQINDAVNEIQQHGCVCKGVEQGLLDFPCLL
                     GEEIVFLCWQYGEPEITYWHRIEDGFAGRRPLLDPSAAGSSGGAGLH"
     gene            12978..14147
                     /gene="queG"
                     /locus_tag="VF182_15730"
     CDS             12978..14147
                     /gene="queG"
                     /locus_tag="VF182_15730"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX6438587.1"
                     /translation="MTADSDPSLAKRSQRIKNEAQRLGFELVGISPARVPPHEESFAR
                     WLREKYHGHLDYMERTELLRRDPRLLVPWAVSIISVGMNYHTPFKRLTPPAPGRGWIS
                     RYAWGADYHEIIRNKLEALLMFVREIGGETMEGRAFVDSAPILERDFAGTAGIGWTGK
                     NTQLISPKKGSWFFLGELFTSLALAYDQPIRDRCGQCDLCLKACPTGAFVGPYLLDAR
                     RCISYLTIELKGFIPRHLRPLIGNHIFGCDICQEVCPYNVKASSTEEPSYMPRQGLYA
                     PDLIELLSIDEKEFRRRFHGSPILRAKRRGLLRNVAVALGNNAGLQAVPALTDALNDQ
                     EPLVRGHVAWALGRIGSSEALAVLRQKLLSEDHPEVKSEIEEALCEAGNPVADQL"
     gene            complement(14169..14612)
                     /locus_tag="VF182_15735"
     CDS             complement(14169..14612)
                     /locus_tag="VF182_15735"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX6438588.1"
                     /translation="MSSTKPSHSNVTTYRVIYGDTDQMGVVYYANYLRWFERGRGEWL
                     RACGIPYQTIERCGMHFPVAELSCRYFRSAHYDDLIVIETRITSVSRASLTFAYRIMR
                     EGEAKLLASGSTRHACVDGQGRIMRIQKELMKTLEVMLYPAAEND"
     gene            14763..15230
                     /locus_tag="VF182_15740"
     CDS             14763..15230
                     /locus_tag="VF182_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438589.1"
                     /translation="MNRTTGFWTLATGSLLLAGGLVACSSVQEQTAAPEQKPAPAQAK
                     SDAASKQPPETKATKSETTQTKSKAVEVEGVISSVGQRTITFQVERKGKVREEVVGVD
                     DKTNIEKAGNKVKLKDLQQTDKALINYEPDAYTPATAIKVTGQGKLQKVGGDD"
     gene            complement(15227..16117)
                     /locus_tag="VF182_15745"
     CDS             complement(15227..16117)
                     /locus_tag="VF182_15745"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6438590.1"
                     /translation="MNGHLGLIHAIIPATVDIHVSIPEDHPSTKNLGSERTGSGTIVD
                     PEGYILTVNYVVTGASSITVTVAEGDQYPGEIITQDLDSGLALLKIPARGLPFLKPPT
                     EPAELGQAALIVASSGNNERKVSGGYVTSLEPYDGHWEYMLDQTVRVTAVNPGFGGGT
                     LANFKAEMLGVVSLNLADVGKFSLAIPISYYIDQEQAFKQRSLVSQKPWLGFYPHLLA
                     GHVVVGGVTEGSPADRHGLKEGDIILDVESTQIRSRRQLYQELWKKRPGERISLRVLR
                     NETSIELNLIGADRRSRYRT"
     gene            16608..17147
                     /locus_tag="VF182_15750"
     CDS             16608..17147
                     /locus_tag="VF182_15750"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX6438591.1"
                     /translation="MKIPVDQITQSPKEISFAEKIESLNSFFANEKHRDFHFPSVLKG
                     SLVYYRSGQELFFKGSFGGSFEGCCSRCLAHYSFDVEKSFDIVLVPDPARPERKVEEL
                     RREELGLSYYSSDMIELAPLVEEQVLLALPTRPLCREDCRGLCGGCGVNLNIEDCACD
                     PAAPSESHFAIFRTLKINR"
     gene            17208..17390
                     /gene="rpmF"
                     /locus_tag="VF182_15755"
     CDS             17208..17390
                     /gene="rpmF"
                     /locus_tag="VF182_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX6438592.1"
                     /translation="MPVPKRRTSKRVKNQRRSHDGLTQPQWSTCSQCGETVLPHRACR
                     ACGFYRGRAVLKVDES"
     gene            17405..>18098
                     /gene="plsX"
                     /locus_tag="VF182_15760"
     CDS             17405..>18098
                     /gene="plsX"
                     /locus_tag="VF182_15760"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX6438593.1"
                     /translation="MPKIAVDAMGGDHAPGVVVEGALLAAQDVNAELILVGDKLAVEK
                     ECLRLAGKLPALPIVNASQTVPMDESPSVALRKRDSSMKVAFEMIKRGEAQAVVSAGN
                     SGAMMAIGMLVIGTLPQVTRPAILAIVPGPSKGTVIIDAGANVDCKPRQLLQFGIIGS
                     VYAEHGLGIVSPRVGVLSNGEEDSKGNELTRAASELLDSAPVNYIGYVEGRDFFNGKV
                     DVIVCDGFTGNVA"
BASE COUNT         4277 a         4516 c         5100 g         4205 t
ORIGIN      
        1 ggtgacttat ggagatccat gcaacgcttc ctgttgcttt atcctgttag gctagctaca
       61 aaatagagca actctcctaa cagttctgtt aaataaggag cgcttcaatg gcacagaagt
      121 atgtggatag cttcggcgcg cgcaaggtgc tgaaagtcgg cgatcagaca tatgatattt
      181 tccggctcga tatgctcgag aacgccggct tcaagaatat ttctcgcctg cctgtatcgc
      241 tcaaggtttt gctggaaaac ctgttaaggc aggaagacaa ccatcacgtt accaaagccg
      301 acattgaagc actcgcaaat tggaatccca aggtcaaatc ggaaaaagag attgcgttta
      361 tgccggcgcg ggtgctgatg caggacctta cgggagttcc tgccgtggtt gacctggcgg
      421 cgatgcgcga ggcgatgaag cgtttgggcg gcgatccgaa aaaaatcaat ccgctagctc
      481 ccgtcgattt ggtgatcgac cattcggtcc aagtcgattt tttcggcacg gcggacgcgt
      541 tgcacaagaa ttcggaaata gagttcaagc gaaatgtcga gcgctatgct ttcttacgct
      601 ggggccaaac ggcttttgac aattttcgac tgatcccgcc cgataccgga atttgccatc
      661 aggtcaacct tgaatatctg gcgccagtgg ttttccgcgc gcaaaaggac ggcgcgcact
      721 tcgcttaccc cgataccgtt gtcggcaccg attcgcacac cacgatgatc aatggtttgg
      781 gtgtagtcgg ctggggagtg ggtggcatcg aagctgaggc agccctgctt gggcagccga
      841 tcatcatgct catccctgaa gtgattggct ttaagctcca cggcaaactt ccggaaggcg
      901 caaccgcaac ggacctggtc ttgacggttg tgcagatgct taggaaaaag ggagtggttg
      961 agaagttcgt tgagttttac ggcgcaggtt tgtctagtct tagcttggcc gatcgcgcga
     1021 ccatcggcaa catggggccc gaatacggcg caaccatcgg ctattttcca attgatgacg
     1081 agaccttgcg ctatttggaa ctcaccggcc gtgatccgca cctgatcgaa ctagtcgaga
     1141 gctatgccag agagcagggc atgttccgca ccgattcttc gtcggatcct atgttcaccg
     1201 attcactcga gctcgatctg gcaaccgtgg tgccaagcct gtcgggtccg cgccggccgc
     1261 aagaccgcat accgctgact gaaagcaaaa aatcgttcaa agaagcgctg ccgtctctca
     1321 tgaagagtgg ggacgccaac aagacggtca ctgtgcagaa taatggcgac agcttccagc
     1381 tcacgcatgg ttcggtggtg atctcggcaa ttacaagttg cacgaataca tctaatccgt
     1441 cggtcatgat cggcgccggg ttgctggcca agaaggctgc cgagaagggt ttggcgagta
     1501 aaccctgggt caaaaccagt ttggcgcctg ggtcaaaagt cgtgaccgag tatttaaagg
     1561 acagcgggct tctgccgtac ttagaagata ttggctttca tctggttgga tatggctgca
     1621 caacctgcat cggcaatagc ggtccgctac ccgagccggt ggttaatgcg atccacgaag
     1681 gcgatttagt tgccgctgcg gtcttgtccg gcaaccgaaa ctttgaaggc cgcattcatc
     1741 ctaatgtgcg cgccaattat ctagcttcac cgcctctggt agttgcctat gcgctggctg
     1801 gtcatatgga tacggatctt tacaatgaac cgttgggaac tgattcagac ggtaaggcgg
     1861 ttttcctgcg agacatttgg cccactccgg aagaaattca agaagtgatg cgcaaatcgg
     1921 tgcggtctga aatgttcaga acagagtaca gccgggccac cgaaggcgat gagctatggc
     1981 agaacatgcc ggtgccgcgg ggcgagctct tcgcatggga tccgcagtcg acttacgtac
     2041 gtgaagcacc ttacttcgat ggaatgtcca gaactccggc cgagctgaaa gagattaaag
     2101 gagcccgcgt gttggcgttg gtcggcgatt ccgttaccac ggaccatatt tcgccggcgg
     2161 gttccatcga gagaaatggc ccggcggcgc gctatctcac caacaatgat gtgttgccta
     2221 aagatttcaa tcaatatggc gcgcggcgtg gtaatcatga agtcatgatg cgcggaacgt
     2281 ttgcgaacat tcgtttgaaa aatatgttgg cgccgggcac cgaaggcggt gtgacggttc
     2341 atttgccgga caagaagcca atgtcaatct acgatgccgc gatgcaatat caaaaagaag
     2401 gcgtcccgct catggttgtc gccggcaaag agtacggctc cggttcctcg cgagactggg
     2461 cggccaaagg tcctcgattg ctaggcgtga aggcggttat cgcagagagc tatgaacgta
     2521 tccaccgtag taatttgatc ggcatgggga ttgtgccgct gcagttcaag cctggtgaaa
     2581 acctcaaggc tcacaatctc accggcttcg aaacattcga aatcgtcggg atctcggcag
     2641 gcctcaaatt gcgacaaact ctggcggtga gagcgaccaa agatgatggc gagattcaag
     2701 agttcacggt tatttgccgc atcgataccc cggcagagct tgaatattac cgtcacggtg
     2761 gaattctaga gttcgtcctc aggcaactct tacaaagctg atcgttggaa cgaaagattc
     2821 gggtaagttt gatatcgctt gagcttgacc tccgccgttt gtaaaccaat gccatgaatc
     2881 ggagacaagc gctggcgtta atcggaccgg gttcgctggc gcttctcctc ggcaattcgc
     2941 gattattccg taagcatccc tatgccgctg aaaggccgat catgaaccaa ccagcgcaca
     3001 tcgcacattt taccgcggat aacccaggca tgatgacctt gcaggggacc aaccaataca
     3061 ttgtggggaa ggacagcgcg gtggtcattg atgtggcatt gagttcggac ggaaacatgg
     3121 atggcatcat cgaacagggc gaagccatgg gcgtcaaaaa gatcgaaaaa atccttttga
     3181 cgcacatcca tatcgatcac tgtggcgggg cgctggcgct gaaaaagcgc accggcgcca
     3241 agctcggcat tcaccgctcg cgcgccggct atctgggcgg ggaggacttc cagtacgacg
     3301 atcaggacag gattgctttt ggcggcggcg agctccaagt gctgcacacc ccgggtcatg
     3361 aatcgggtca ctgctgcttt tatgaatcag gtgataaggt gctgttcagc ggtgacaata
     3421 tcttgggata tggaacggcc gtgattcacc cgccggacgg taacatgacc gattacttgc
     3481 aatcgttgga gcggctcttg gaatttaata tcgatctgat tctgcccgga catggaccgt
     3541 tgatcgggaa accggaggca aagatcaggg aatatttgcg tcatcgcatg atgcgcgaga
     3601 agcaggtgat tgaagccatg cgcaacggac ggaatacgat cggcgatatc acccaatcga
     3661 tctatgtgga tgtgtccccc gctctgcaga gggtcgcgga attctcggtt caggcacact
     3721 tggagaagct aatccgcgat ggccgggtga aaagagaaga tggccgctat caaatgacta
     3781 ccgactaaac acttcgtttc cgtgccggat caaaggacat caatcgggct tggcttttgt
     3841 tgctttcccc tgcctctctg tatgtagcaa gaagtagaag aactaactgg tgcgttgttg
     3901 actcaggttg gcgccgctgt actgatcgtg ttcctctacc acttctccag aataaccacc
     3961 ggatatccgg cttgtgtcac ccgccgcgct tgttcttcgg cgctggagcg agtggaaaat
     4021 tttccgagtt gaacgcgata atatgtggtc gagctggtgt tcaagggcac aatggcaacc
     4081 tctgaaaatt gtcttgcgac aagatcgcgc agctggcgag cattctcgag gaccgaaaac
     4141 gaccccagct gaagggtgta atcgagcgaa tctttaatca acgatacttt gtcagcgccg
     4201 ctaccgacga cttcaatccg caccgggatg gttccgggac caaccatgcc gaggctttcc
     4261 gctgcggcgt aagataggtc tataatcctt cccttcgcga acggcccgcg atcgttgatc
     4321 aaaacttcta cggatcttcc attctccaaa ttggttacca ggactcgagt gccgagaggc
     4381 aatgtctggt gcgccgccgt gaggtcatgt tgattataga tgacgccgct ggcagtggcc
     4441 ttgccgtgaa aaccgggccc ataccaggag gcgatgccgg tttgactggc gcgcggcggt
     4501 gtgatgggag gaggaattct cgatggcggt acggaacatg ccgttatcga gatgactgtt
     4561 actaagagta aaccgaggct taaatcggaa atcggcttca cattgcccac tgcggaagag
     4621 ggtccggaga tatcgctact agaccactgg taccaagcgc attgaaggcg agtcacggcg
     4681 gcggattccg aactatttag cctagcagtg cttccatcct gtaggctccc tgtttccgat
     4741 ccggagcagg aattctttct ttcgcgctta ttaaactacc aaacacggca tccgccaacg
     4801 gcaaaacaac attgagattg ttcaggtgcc gtttgtgatg catgtgatga tgcgcgtcaa
     4861 gccagcgaaa ccacttggtt tgttcgatgc ggcgcccggt aggaatgtgc atgcagtagt
     4921 gcaagtactc gtagagccca taataaaccg ccatcgccgc gcaggcgccg agaagtatgg
     4981 gcgcgcctaa aagatcctgg atcaggagta tcgccgggac gtggagggcg ataatgaacg
     5041 gcgcgttcca ccaagcaaag cgaacctttc tccgatattt cttatccgag agaaagtaat
     5101 cggctcccgt gcggaaaaga ccgtggtgaa ccagcgcgtg cgcgcgaaag ggatacttcc
     5161 agcgcggttg atgcatgagg tatttatgta acgtccattc gaaaaagctt ccgtacacga
     5221 aaaaggcggc aaacgcggcg acttcgttag tgtagaatga catttttttg ttcggctccc
     5281 tgtatcacaa ccgagtcaac gggttctcga gcgattcgtt tattggaagg cttgtgacgc
     5341 cagttggcaa ggcggaatga agatgtctaa ttgatgagtt cctgttgagc tcatgaaaac
     5401 gtccttctca cactacgtgg ttttgtcgga atggtcaatt gcaatgcttt aaaaggcgta
     5461 ttatcaacaa ctaccgccgt ttacccagac cggagagtgt gctactttac cggcaactta
     5521 caagggagtg tactagttta tgtcaggcca ttccaaatgg agcacgatca agcataaaaa
     5581 agccgcaaag gacgcgaaga agggtaagct attcaccaag ttgatcaagg aaattacggt
     5641 tgcggcccgt atgggcggcg gcgatatcaa cgcgaatcct cgcctgcgga ctgcggtatt
     5701 caccgcccgc tccgcaagta tgcccaccga gaacatcgat cgagcggtca aaaagggaac
     5761 cggcgagctt gaaggtgtca cttacgacga gattcaatac gaaggatatg gccccggtgg
     5821 cgcagccata ttagcccatg tgctaaccga taacaaaaat cgcacggttt cggagattcg
     5881 tcgcctgttt accaaacacg gcggcaatat gggggaggcc ggctgcgtcg cctggatgtt
     5941 cgacaaaaaa gggctgatcg cgatcgagaa aacgcagatt gaagaggatc gcttgatgtc
     6001 aatcgttctt gatgcaggcg cggaggacgt cagggacgaa gatgagatct atgaagtgat
     6061 cacaacggtc gaagagttcg acaaagtcaa agaacgactc gaagtcgaaa aagtatccgt
     6121 cgcgagcgcc cagatcacga tggtgccgaa gaacactgtc gatgtcgatg agaagaatgt
     6181 cgaacagatt ttaaaactta ccgaggagct tgaagatcac gacgatgtcc agagcgtttc
     6241 ggctaatttt aatattccga gcgagttaat ggagaaggct agttgaggtg gccagcgcct
     6301 gtaagaagca gagatgatgc ggctccgctt gcgcgcaggg ttctggggat tgatccgggt
     6361 tcggtcataa ccggttgggg agttgttgaa gcggcaggca actcattgat ccatctggcg
     6421 catggcacga tcgctacagg aggcgctcag gggcaagcgg ctcggctcga acggatttac
     6481 cgcggccttg aggaagtaat tggctccaat aatccggatg gtgtctgcgt cgagcgggtt
     6541 ttttttgcac gaaacgccca gagcgccctc aaactgggcc aggcacgtgg cgtcgccatt
     6601 ttggctgcgg cgcgaaacag cttggcggtt catgagtatg ccgcggtcga gatcaaagcc
     6661 gcggtagtag gctacggcca tgcaaccaaa cagcaagtgc aaatgatgat tgcgtctttg
     6721 ttgcggctta acggcaagtt gcccgccgac gccgcggacg cgctagcggc ggctatttgc
     6781 catctccaca tgcagacatt gcgggcgcat ttggtcgaag ctctgcccaa agatattgcg
     6841 ccgttgccgc gcaaatcgat ccttccttag aggcccgacg gcgctacagc taaagcgaaa
     6901 gattaagcag tcgtgatcgc gcaaattagc ggcactctgg cgcagaaatt ggcaggagaa
     6961 atcgttgtcg acgtcggtgg agtcggctac caggtcttca tcccgctaaa cgtgttttat
     7021 catctaccgg agatcggtgc gccggtccgg ctccatattc acactcactt gcgcgaggat
     7081 gccttgcagc ttttcgggtt ccaggagcca gcggaaaagc aagttttctt gcagctcaac
     7141 ggcgttgccg ggatcggacc caaactcgcg ctgaatatat tgtcgggaat cccggccgaa
     7201 gagctcaccc gcgcgattaa agaaggcgat cagttgcgct tggtatccat tcccggagtc
     7261 ggcaagaaac ttgcggagcg catgatcgtg gagcttcgcg atcggtttgc ttttctcgaa
     7321 tctcaacccg ccgaaattcc ggagcaagga cgcgacccgc ggctgatcca ggacgctgtt
     7381 tcggctctcg tcaatctggg gtatcgtaaa ggcgaagttg aaaagaacgt ccgtgactgc
     7441 gttcaagcag gcaatcacag cctcgagcag gtaatcaaag aagtacttcg gcgcatgagc
     7501 cggtgatatc gattatgagc tacccaccga ttgttaatcc ggggaatacg gagtcacggg
     7561 agatctcgcc ggcgcgaagc gacgaggatc tgaactatga agtaaatctg cggcctcggg
     7621 gattcgagga atacgtgggt caagatcgtg tcaaagaaaa cctcaaggta gccatcgacg
     7681 cagcccgggg tcggcgggag gttttagacc accttttgtt tcatggcccg cccggattgg
     7741 gaaaaacatc actggcctat atcatcgctc gcgagatggg ggtgaatatc aaagggacct
     7801 cagggccggt tatcgagcgt gccggtgatc tggcggcaat tctcaccaat ttggaggaag
     7861 gcgatatttt gtttatcgat gaaattcatc gcctcaacca tattgttgag gaagtcctat
     7921 atcctgccat ggaagatttc cagatcgata ttatgattgg tcaagggccg gcggctcgat
     7981 ccatcaagct ggagttgaag ccatttacat tgatcggcgc aacgacaaga tcaggtctgc
     8041 tcacctcgcc actgcgggat cgtttcggct tgagcttccg cttggatttt tattcaccag
     8101 aaaccctgac tgatcttctc cagcgctcgg ctgtaattct tggtgtgggc ggcgagcagg
     8161 aaggcatcgc cgagatcgcg cggcggtcgc gcggcacgcc acggattgcc aaccgcctgc
     8221 tgcgccgtgt gcgcgactat gcgcaagtca aagcagcggg acggatcact ctcgcggtca
     8281 cggtcgaggc cttacaaatg ttggaggtgg actctatcgg tctcgataaa atggatacca
     8341 tgttggtatt gaccttgatc gagaaatttt ccggaggacc ggtcggagtc gagactttgg
     8401 cagcggcgat tggcgaagaa aaagatacca ttgaagatgt ctatgagcca tttttgattc
     8461 aggcaggttt tatccagcga acgccccgag gacggatggc gacgcctctg gcttacgatc
     8521 acttcggacg aaaatccaag ggacaaaagg gcactgcccc aaagaaccaa cccactttgt
     8581 tttgatctaa caacgccctg agactccgct gcctcagagc gggatcaatc ttatgcctga
     8641 gttcggcaga actttgataa ttctggggat cgtaatcatt gccctcggag tgctgtttac
     8701 ttttggcggg aaaatcccct ggctggggca tttgccgggg gatattcacg ttcagcgagg
     8761 tcgcttcagt ttttactttc caattagcac gtgcattgtc atcagcgtcc tcatcagcct
     8821 tgtgctctat ttgtttagaa ggtaaaattt ccttttgaat ttagctcctt agcaaaatgc
     8881 catgtccaaa atctgggata gacaaccggt aatcagcgat acgccccgga tcgacgaagc
     8941 caaacggcgg aagcgtgaag gcctggcaat tctcatcact tcgctggtgg tgctggcgtt
     9001 tgcttttttc gaggtacaac ttcccgatgt ttcgtcggaa tactctctca gtaataacat
     9061 cgtttttttt ctcttaatca acatcaatat cattctgctg attctcctgg tttttctggt
     9121 cgtcagaaac ctagtgaagt tggtgttcga acgcaaacgc cgcatcctcg gttcgcggct
     9181 acgcgctcgg ctggtgatcg cattcgttgg gctgtcgctc attccgacac tgttgctctt
     9241 cgtcatcgcc ggcggatttg tcactcgctc ttttgaaaga tggttcgata tacaggttga
     9301 gaccgccctc cagggatcgt tggaaatcgg tcagacgtat tatcaaaact ccgcaaataa
     9361 tgcacttttc tatgcgcggc agctaagtcg gcgcgtcact gaacagggat tgttcgacag
     9421 ccagcggcga agcgatctta aagaattcat tcagagtaag cagcgtgagt acaatcttgg
     9481 cactgtcgaa ttgttttcac ccgaccacct accggtcgtc gtggctttca atgatcaggt
     9541 gccgaccggt gttagtatca aggctgagcc cgattttctt aaccgggcgc tgcgcggttt
     9601 ggaagttacc aggactcagg ctttcggcga cggcgacatc atccgcggcg gtgtgccgat
     9661 ctatgacagc ggtaagcgga ttcagggtgt ggtcgtagtc gactattatg ttccgaagag
     9721 catcaccaag cgggcgcttg agatctcccg ttcctatgag caatacaagc accttggttt
     9781 tctgaagaac ccggtgaaaa acagttacat tctgactctg cttttggtca ccctggtgat
     9841 tattttcgcc gcgacttggt tcggccttta tcttgccaag ggaatcacaa tcccgatcca
     9901 gcaggtggcg gcgggcaccc atgaagtggc tcacggtaac tgggattacc aaattgaggc
     9961 ggcgggcgac gacgagttag gaaccttggt tgagtctttc aaccagatga cccgggactt
    10021 aaagaagagc aacttggaac tgttgcgccg cggcaagttc gttgaaacac ttctggctaa
    10081 tatcactgcc ggagtgattg ctgtggatcc gactggaaaa atcacttctt ggaacaaggc
    10141 agcagaacaa atgctagatg tggatgctgc gactgcgctc ggaaagaact acggggaagc
    10201 ctttgaaagc gagccattaa cggtgatgcg ggccttgctt gacaatctca atgatcgcga
    10261 aagcagcgca accgaattgc agatacagct gccggatcat ctccttacgg tatttgcgac
    10321 cgcggctacg ctcagagacg atagcaacga tagtttaggc atcatgattt ttctcgagga
    10381 catcactcag atccaaaaag tacagcgaat ggaagcctgg cgcgaagtgg cgcggcggat
    10441 tgcccacgaa atcaaaaatc cactcacgcc gatacagctc tccgcagaga gactgcgcaa
    10501 acgctatgct aaactgctgc aaggagacgg cgcagtgctg gacaaatgca cgacaacgat
    10561 tatccagcag gtcgtcgagc tcaaaaacct ggtgaatgag ttttctcaat tcgcgaggtt
    10621 gcctacggcg cagccggtgc cgaacgatct gaacgggatc gttcaggacg cgctattcct
    10681 cttcaaggaa ggacataatc atatcgattt tgactttcgc cagggggagt tgccagcgct
    10741 ggaacttgat cgggagcaaa ttaagcgggt catgattaat ttgttggaca atgccgttgc
    10801 cgctgttggc gagggcggcc atgttcggat atcgacgtcc tacgatcacg ctcggaatac
    10861 agtttacctc gaagttgctg acgatggttg cggcatgccg ccggaagctc gagttagaat
    10921 attcgaacgt ggattttcca caaagaaaca gggtaccggc ttgggactga ctatcgtcaa
    10981 tcaaatcatc gaagatcatc gcggttacat tcgggtgcga aacaacgagc cgcgtggcac
    11041 cagattcgtc atagagttcc cggtgcggca gcaggacgcg ctgcacgagc tcaaaaggaa
    11101 agctgtgcag ctatgaaaaa cggaggggcg gtgagcgact tatcatctca gaaaaccatc
    11161 ctgattgtcg acgatgagga caaggttcgc gaatcggtca aagaagtgct gacggacgaa
    11221 ggatatcggg tgctcgacac cgccgatgcc acgtgtgccc tcgagatgat tgaagcggag
    11281 caacctgggc ttgtgctgct ggatatctgg atgccgaaaa tggatggcat cggcttgctc
    11341 aaggaaatca agagcaagaa gccggcaacc aacgtagtca tgatctcggg gcacggcaat
    11401 atccacaccg cagtgaccgc aacaaaattt ggcgctttcg atttcatcga aaagccggtg
    11461 tcgctcgatg gcctgcttca taccgtacaa cgcgcgttgg gggagcttcc tgttgctcag
    11521 agcgcgaccg aaatgggcca gagtcacgga gccgaggata ttgccgagtc agtgagtgcc
    11581 gcccggcgga gtagcgcatg cgccgaaccg attcggcaaa agaccttgag aacgagcgtg
    11641 gtgagttcag gccagggact gcattcaggg attaaaacag ggttgattct tcatccgcta
    11701 ccgccaaata gcggcatctt atttgagggc atctccggtg acgtcagcgt acctgcgcat
    11761 ttggattatg tcggatcgac gggcttcgcc acatcattga gccatcgcgg ctttacggtg
    11821 gccaccgtag aacatttttt agcggtgctc catagttacg ggattacgaa cttgctggtg
    11881 aaagttcaag gcgaggttcc gatattggat ggatcggcag cggagttctg tgaactcatc
    11941 gaagaagccg ggctgcacga gcaagaggaa agctggtcta gaattgtcat cgatcggccg
    12001 tatcgtttcg gtgaggacgc aggcgaatgg atcagtatcg agccagcgga gaaattttcc
    12061 gtccattacg ttctgaatta tcccaaaccg gtggggatgc aggagtatct ctttacccat
    12121 cagggtccga attcgttcaa agaagaaatt gcgccggcaa gaaccttcgg gtttttgcgc
    12181 gatatggaga agatgcagaa gctcggcttg atcaacggtg gtaagcttag taattgtatt
    12241 ttaatcgatg atgagaaaat cgtgaacacg gatttgcgct ttccggagga actggcgcgt
    12301 cataaaattc tcgacattat cggggacttt tatttgcttg gcagacccat tcgcggcaaa
    12361 atcacggctc acatgactgg ccattcggat aacattggac tacttaaagc tgttcgagct
    12421 gggatgcagc tttagggtgg gttcgttgat cggctatgga ctcctcttcg ccctattcca
    12481 gattgtttac gatcaacgag gccagcgcgc ttttgcccgt agtgcggccc accatcgaaa
    12541 aaatcttgga gatcatccgc cgtttacggg ccaagagcga gatagtgatt cgcgcggagc
    12601 aaatcgatcc ggaaaccccc aatctgctgc gccgtttaca gcagaatgag gagatagcag
    12661 ccctgatcaa gcagatcaat gatgctgtta acgagattca acaacatggc tgcgtgtgca
    12721 aaggcgtcga acaaggattg ctcgacttcc catgtctctt gggtgaggaa atcgtctttc
    12781 tctgttggca gtacggcgaa cccgaaatca cctactggca taggattgaa gacggttttg
    12841 ctggccggcg cccattactg gacccaagcg cggccggctc gagcggcggc gctgggcttc
    12901 attaaaatcc ttttcgcatg gggatctcct aatcattttt ccctcgtcat cttgtttgct
    12961 agttttctga ctctgttatg acggccgact ctgacccgag cttggccaag cgttcccagc
    13021 gcatcaagaa tgaagcgcaa cggcttggct ttgaactcgt tggaatttct ccagcgcggg
    13081 tgccgccgca cgaggagtca ttcgcacgtt ggctgcgcga aaaataccat ggtcatctcg
    13141 attatatgga gcgcaccgag cttttgcggc gcgatcctag gctgcttgtc ccctgggcgg
    13201 tgtcaattat ctcggtagga atgaactatc acacgccctt taaacggcta actccgccgg
    13261 cgcccgggcg tggctggatc tcgcgctatg cttggggtgc cgattatcac gagatcatcc
    13321 gcaacaaact ggaagccttg ctgatgtttg ttcgtgagat tggcggcgag accatggaag
    13381 gcagggcttt cgtcgattcc gccccgattc tagagcgcga ctttgccggt accgcgggga
    13441 tcggttggac tggcaaaaac acccagctga tttcccccaa aaaaggatcc tggtttttcc
    13501 ttggtgagct gtttaccagt ctggctctgg cttatgatca gcccattcgt gaccgttgcg
    13561 gtcaatgcga cctgtgttta aaagcctgcc ccactggtgc cttcgtcgga ccctatctat
    13621 tggatgcgcg tcgctgcatc tcctatctta cgatcgaact caaaggtttt attccacgtc
    13681 atctaagacc gctgatcggt aatcatattt tcggctgcga tatctgccag gaagtttgtc
    13741 cttataatgt caaggcaagt tccaccgaag aaccctctta catgccgcgc caaggtcttt
    13801 acgcgcccga tctgattgag ctgctgtcga tcgatgaaaa ggaatttcgc cggcggtttc
    13861 atggcagtcc cattctacgc gcgaaacgac gcggcttatt gcgcaatgta gcagtggcgc
    13921 ttggcaacaa tgccgggctt caagcggtgc cggcactgac cgatgcactg aatgaccagg
    13981 agccgctagt acgaggtcat gttgcatggg cgcttggccg gattggttca tccgaagcgc
    14041 tcgcggtgct gcggcagaag ttgctttcag aagaccatcc ggaggtcaaa agcgagatcg
    14101 aggaggctct ttgtgaagcc ggcaaccctg tggctgatca gttgtaacgg cgtggttgta
    14161 agcaggcatt aatcgttttc tgcggctggg taaagcatca cctcgagcgt cttcatcaat
    14221 tccttctgaa ttctcatgat tctgccttga ccatcgacac acgcgtgtct agttgagccg
    14281 gaggccagca gcttagcttc accttcgcgc ataatccgat atgcaaacgt taatgacgcg
    14341 cgagagaccg aagtaatccg agtttcgatg acaatcagat catcgtaatg cgccgatctg
    14401 aaataccgac aggacagctc cgcaactgga aaatgcatgc cgcatcgctc gattgtttgg
    14461 tacggaattc cgcatgcgcg aagccattca ccgcggcctc tttcgaacca gcgtagatag
    14521 ttggcgtagt aaacaacgcc catctgatcg gtatcgccgt agataacccg ataggttgtt
    14581 acattcgaat ggctcggctt ggtcgaactc atacggtctt ccttgtatgt tatgttaagg
    14641 cttttctcaa agccatcgct ctcgatgcgc cttgacaatt tgaaatgaag tatgctttaa
    14701 gttctacaaa tttctggcga ccattttcgt gatcgtcggg tacgcgattt cggaggaatg
    14761 aaatgaaccg aacaactgga ttctggacct tggcaaccgg ttcgctgttg ctcgccgggg
    14821 gattagtcgc atgttcgtct gttcaggaac aaacggccgc tccggaacaa aagccagcgc
    14881 cggcgcaggc caaatccgac gcggcatcaa agcagccccc ggaaacgaaa gctacaaaga
    14941 gcgaaacgac acaaaccaag tcgaaagccg tcgaagttga aggtgtcatc agcagtgtcg
    15001 ggcagcgcac gataaccttt caagtggagc gcaaaggaaa ggtccgcgag gaggtggttg
    15061 gagtggatga caaaacaaat atcgaaaaag ccggtaacaa agtcaaatta aaagatttgc
    15121 agcagactga taaagcgttg atcaactatg aacccgatgc ctacactccg gcaacggcaa
    15181 tcaaggtgac gggacaaggt aaattacaga aggtcggcgg tgacgactaa gtgcgatagc
    15241 ggctgcgccg gtccgcgccg atcaagttta gttcgatgct ggtttcgttt cttaggaccc
    15301 gcagggaaat tcgttctccc gggcgcttct tccaaagctc ctgatacagc tgccgccggg
    15361 agcggatctg cgtactctct acatcgagaa tgatatctcc ctctttcagt ccatgacggt
    15421 ctgccgggct tccttccgtt acgccaccga caaccacgtg tccggcgagc aagtgcggat
    15481 aaaagccgag ccaaggtttt tgactgacca gcgaccgctg tttgaacgcc tgctcctgat
    15541 cgatgtagta gctgatcgga atggccaatg agaattttcc aacatccgca aggttcaggg
    15601 aaacgacgcc caacatctct gctttaaaat tggccaaggt gccgccgccg aatcctgggt
    15661 tgacggctgt cactctcacc gtttgatcga gcatgtattc ccaatgtcca tcgtagggtt
    15721 ccagtgaagt gacataacct ccactgactt ttcgttcgtt gttaccgcta ctggcaacga
    15781 tcagagcagc ctggccgagt tcggccggtt ctgttggcgg tttgaggaag ggcagtccgc
    15841 gagccgggat ctttaaaagc gcgagcccgc tatcgagatc ctgggtgatg atttcacctg
    15901 gatactggtc cccttcggct acagtgacgg taatggagga agctccggtc acgacgtaat
    15961 tgaccgttaa tatgtagcct tctggatcga cgattgttcc ggatccggtg cgctccgagc
    16021 cgaggttttt ggtcgacgga tgatcttccg ggattgacac gtggatgtcg accgttgccg
    16081 gaatgattgc atggatcaag ccgagatggc cgttcaaagt cctgctcctt acgcgagttt
    16141 acccgccgct attgcgcgag catgtcgccc gattttaagt gcgcggcttc acgccattcc
    16201 aacgctctct cgtgaattct attgatgatt gcgagcttgt caacggtcgc ttggtggctt
    16261 gcgtatttct tccgtgacat atgttgatcg agtaggattt ttttaatcgc gcgattgtcg
    16321 ccgcttgaga agcggggcca aggatccaaa ctactcgagc cccgacgctg gctatggatg
    16381 ccgcacttga accgcagcct caatagtgat ggggcatcaa ttcatgcgac gatggcaaat
    16441 agcaggaagg gcttttcgga gcgcagtgtt gtccgcaact aaagagggcc agcgccggaa
    16501 gttgcggata aaccacatac ttttgcatgt tagtcatggc cgccaacttg acaacagagc
    16561 agaatccatt atattcaaaa tcgtccggcc aacctgattc tctcgctatg aaaattcctg
    16621 tcgaccagat aactcagtct cccaaagaaa tctcgtttgc ggagaagatt gagtctctca
    16681 attcgttttt cgccaatgaa aagcatcggg attttcactt cccatcggtg ctgaagggaa
    16741 gtttggttta ctacagatcg ggccaagagc ttttttttaa ggggtctttc ggcggaagtt
    16801 ttgaaggctg ctgcagccgt tgtttagctc attattcgtt tgacgttgaa aagtcgtttg
    16861 acatagtgtt agttccggac ccggcgcggc ccgagcgcaa agttgaggag ttgaggcgag
    16921 aggagctagg tttgagttat tattcaagcg acatgatcga gctagcgccg ttagtcgaag
    16981 agcaagttct actggccttg ccgacccggc cgctgtgtcg tgaagattgc cgaggattgt
    17041 gtggtggttg tggagtgaat ctgaacatcg aagactgcgc ctgcgatccc gctgcgccct
    17101 ccgaatcgca ttttgcgatt tttcggacgc ttaaaattaa tcgttagtaa ctgccaattc
    17161 tctgaagatg ggttctgccg tttgccggcg ggaaggatag gatcaaaatg cctgtaccaa
    17221 agagacgaac ttcgaaacga gtaaaaaacc agcgccggtc gcatgacgga ctgacacagc
    17281 cgcaatggag cacgtgttcg cagtgcgggg aaactgttct gccccatcgc gcttgccgcg
    17341 cctgcggctt ctatcgcggt cgggccgttc tcaaagttga cgagagttag cacttcattc
    17401 ctctatgccg aaaatcgcag tcgacgcaat ggggggagat catgccccag gggttgttgt
    17461 ggaaggcgcg ctgcttgcgg cccaagacgt caatgcggaa ttgattcttg tcggggacaa
    17521 gctagctgtt gaaaaagaat gtttgcgcct tgctggcaag ctccccgcgt tgccgatcgt
    17581 taacgcttca caaacagtac ccatggacga atcccccagc gtggcgctga ggaagagaga
    17641 ttcgtctatg aaggttgcgt tcgagatgat caagcggggc gaggcgcagg ccgtggtcag
    17701 cgccggaaac tccggagcca tgatggccat cggaatgctg gtcattggca cgttaccgca
    17761 agttactaga ccggcaattt tggcgattgt gccggggcca tcgaagggaa cagtcatcat
    17821 cgatgccggg gctaatgtcg actgcaaacc gcgccaatta ctgcagtttg ggattattgg
    17881 ctcggtatac gcagagcacg ggttaggcat cgtcagtccc agggtcggtg tgctgagcaa
    17941 tggggaagaa gacagcaaag ggaacgagtt gacccgggcg gccagcgagc tcctcgacag
    18001 cgctccggtc aattatatcg gatacgtcga aggtcgtgac ttcttcaacg gcaaagtcga
    18061 tgtgattgta tgtgacggct ttacggggaa cgtcgcat
//
LOCUS       DASUVS010000287         9477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1209530, whole genome shotgun sequence.
ACCESSION   DASUVS010000287 DASUVS010000000
VERSION     DASUVS010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9477
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209530"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..843
                     /locus_tag="VF182_15765"
     CDS             352..843
                     /locus_tag="VF182_15765"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6438594.1"
                     /translation="MVMVAKRAEDLGLNSVWVLDRMLWPLNPRAPYPIGDGSLPVRYK
                     SVLDPLETLTFTAAHTSRILPWYNPVLLARRLTTLDILSAGRLRIGFGIGWSPDEYGA
                     AGVAWQERGKRADESITALKRIWTTDPVEFQGRYSEIFHRSDACAETSPTDLYGGVYS
                     SCP"
     gene            916..1206
                     /locus_tag="VF182_15770"
     CDS             916..1206
                     /locus_tag="VF182_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438595.1"
                     /translation="MTSKTPQECRARSLRPRAYHCRRRGGSQSADGERPDRFYGTLEQ
                     IAEDFTAARKLGAAEIVIYPQFSSGDRDGKRYRRSHGKAQARNKAKLMAKIV"
     gene            complement(1599..2042)
                     /locus_tag="VF182_15775"
     CDS             complement(1599..2042)
                     /locus_tag="VF182_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438596.1"
                     /translation="MIYGFVLAIEMSLLFHEVAFSASRSFRGSGKFSAPAVRSQHGLT
                     GNFRSGFGVELGPAQRPHRFRDRFDRSDFRSRFGGDVGFIGGHVIDTGDVIIIQVPPS
                     APASSPSRELAHSGTYVDPQWVDAGHGVEILKPGHWSDEKQRVER"
     gene            complement(2627..3622)
                     /locus_tag="VF182_15780"
     CDS             complement(2627..3622)
                     /locus_tag="VF182_15780"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438597.1"
                     /translation="MTSKMLSVLGICVWAVFPASSSPAQSVTLRYGQIPSSIKTVSAI
                     QFVIGQRKGLFEREGIQLNLMPIEGGADNMMVALNDGKVDITRTATPYLIQSVLKGSD
                     GVAIAGETATPIYSLIVKPEIKSFAELKGKTVGLSLAVDTISISTRKLMAMNGITESD
                     FKLRELVGTPARSECLHKGECDAVPLGQPEDLLLMGKGYRRLGVTTDAMPNFQFIVSA
                     VRKSWAEKNKDALLRYVRGLAAAFRFMRDPGNRDEVVKMVIDTTGSSEAIARQTLSLY
                     FDPDRGVIPQQAEIDMKGFDQVIRVMAEAGQLHAPLPAAERFVDLQYLRAAGLMK"
     gene            complement(4013..4993)
                     /locus_tag="VF182_15785"
     CDS             complement(4013..4993)
                     /locus_tag="VF182_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438598.1"
                     /translation="MKLTLAAGHYDRTEALRDGRVRPEGIALTYLTLRVEEIFWRMAQ
                     YREFDASEFSLGGYFVRRGRGADDLVAIPVFLSRFFRHSGLFVNAGAGIEGPQDLRGK
                     RVGVPEYQMTAAVWVRGFLADDYGIRPEDVEWVQGGLEDPGRLPFEPAEPPGVKLGFA
                     PNDRSLAQMLAAGELDALVTARTPSTFRRGGGPVRRLFDNPWQVERDYFRRTRLFPIM
                     HTAVIKREVLDENPWVAVTLAKAFEEAKALALQDLTQTSALPVSLPFLVDHAYETLDL
                     MGDDFWPYGLESNRHTLETFARYMHEQGLTPERLSIDALFPESTHRTFRI"
     gene            complement(5527..6201)
                     /locus_tag="VF182_15790"
     CDS             complement(5527..6201)
                     /locus_tag="VF182_15790"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6438599.1"
                     /translation="MKKPVPIAIALAALLAVSYFAINAINRQPSLTDTFNRIYATGRW
                     ATDAAGKGTSGSGSTLEITREYRAYIEDFIKKHEVNSVIDAGCGDWSYSSAIDWGDAS
                     YLGIDIASDVIVPVRKKYETGKVKFQVGDITEELPAADLLISKDVLQHLSNDLIHKFI
                     KNNLRKGKYKWVILTNDRSSDNQDIPSEHTGGLILPRLRSRSKGSSIFRSSSEKRLRR
                     SPRFST"
     gene            complement(6856..7770)
                     /locus_tag="VF182_15795"
     CDS             complement(6856..7770)
                     /locus_tag="VF182_15795"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6438600.1"
                     /translation="MKSKILGCLMLFLCFFVMSRAIAQDLGAQFKKVREGIYVYAAVL
                     NEANSTIILSQEGVVLIDTGQSPKDSHVVMAAVKKLTPQPVRYIVHTEPHPDHAMGDF
                     VFSPPAVVIGHAGSADSMRASESFSPARIEKLMSTSTEMRAAFNGFRLVTPHIEYRDK
                     LSLKVGERALDLYYLKNIHSEADTAIWLPQERVLFTAASVGVKRFGNHRPSVSIPDTL
                     SAIKMMKALNPEVVIPGHGDPGTTKILDDMESYYNRLMDGVRQMVQQGKSLDEIKKEL
                     KMPGTEDWEGQARLGNNIEAAYRGVMGK"
     gene            complement(7795..8709)
                     /locus_tag="VF182_15800"
     CDS             complement(7795..8709)
                     /locus_tag="VF182_15800"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6438601.1"
                     /translation="MKPKLTFIVLAVLSVACAGLAYSQELPPNLKKIKDGIYVYVGSN
                     FNSNCGIVVTQEGVVLIDSGHNPTDSRSILDAVKKISPLPIRFLIDTEPHPDHTTGHF
                     VFSPPATVIAHEGATQSMINREKETPGRIEKLAGVSPEMRKALDGYRFVPPQVEYRGK
                     MTLNLGERSFELLYLKGVHSEADTAVWLPGERVLFSASGIVVDQFNILRPFVTIPDIL
                     AASKMMKALNPEIVVPGHGTPGTVKIFEDTEKYYALLEERVGKMVAEGKSLDEIKREL
                     KMPEYDHWATKERFPTNVEAAYKVVKGG"
     gene            9035..9427
                     /locus_tag="VF182_15805"
     CDS             9035..9427
                     /locus_tag="VF182_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438602.1"
                     /translation="MVIVSVTRLRLRSLPFSRIPLVHPAIGHTGKTDPGQSCVRVRKS
                     KGLAFWTLEMWRDNQAIRTFEPASPHGGAMQKLPHWCDEAAFADWEQDTADWPPWETA
                     RGKLAATGRLVDVLLPSGQHRAARIETT"
BASE COUNT         2152 a         2636 c         2581 g         2108 t
ORIGIN      
        1 acttgcgtct gagcatgcgc aggcggcagc gcgatgaaga ttccccaaca aaatagcgca
       61 atcattgttc tcgggaaggt gcgagaagtc atgttccgct ccagtctaga caagcatttg
      121 ccggcatagt gcgaagatat atcgacagct ctctcgatct gtcaagatga ttcagctccg
      181 acagagtcgt tatggctttt tacttgtcgc aattcttgta aatccccggt gaggttgtta
      241 acgaagattt tttagcggac atggcatgat tcgccggaat gctttaggag gttatgatgc
      301 gtttgggaaa ttgcacttcc acaaatagga tcagcaatcg gaccggaagc gttggtcatg
      361 gttgccaaac gggccgaaga tttgggtttg aacagcgttt gggtcctcga ccgcatgctg
      421 tggccgctca atccgcgcgc cccttatccc atcggtgacg gttctttacc ggtcagatat
      481 aaaagcgtgc tggatccttt ggagacgcta acgttcaccg ccgcgcacac gagccgcatc
      541 cttccctggt acaatccggt cctgctggca cgccgattga caactctcga tattctctcg
      601 gccggacggc tacggatcgg attcggcatc ggatggtcgc cggatgaata cggcgccgcc
      661 ggtgtggcgt ggcaagagcg cggcaaacgc gcggatgagt cgattacggc cctcaagaga
      721 atttggacca cggaccccgt agagtttcaa ggcaggtatt ccgagatctt ccatcggtcc
      781 gacgcctgtg cagaaacctc acccaccgat ttatatggcg gcgtttactc ctcctgccct
      841 taaagcgggt tgcgaccgag gccaacggct ggttcccagt cggcattccg ctgagcggca
      901 ttggcgcgat gttcgatgac atcaaaaaca ccgcaagaat gccgggcgcg atccctccgg
      961 cctcgagctt atcattgccg cagacgtgga ggttcacaaa gcgccgatgg agaaagaccg
     1021 gatcgatttt acgggaccct cgagcagatt gccgaggact tcaccgcagc gcggaaactc
     1081 ggcgcggcgg agatcgtcat ctatccacag ttttcctctg gagatcgaga cggcaaaaga
     1141 tatcgtcgct cgcacggaaa agctcaggcg cgtaacaaag caaagctgat ggcgaagatt
     1201 gtctgaacta aaccctcgct ctgttacagg atcgccttgc gagacgcacg atggtcggac
     1261 gccgttgcgg ttggcagctt agcttttgtc gagaaggtca gaagcgagct taaaatcaaa
     1321 gccctgcgtc gtgaactcaa gcactcatat ggatatacat gctgagagaa gccgtgaagc
     1381 catagagcaa ttctacttcc aaatgcggcc taagcgtcac acgctgcggt gaaagagaat
     1441 tgccaacgtt gcgctgagtt gtatggtccc aaccacggac accagccagt cccgacaccg
     1501 acgccggccg cggaagagga agaaaggcct aaacgcccac tgcgcgagcc acgtgctgaa
     1561 actaggagct aagatatcag gttgctttcc ggtgaccctt aacgctctac tcgttgcttc
     1621 tcatcgctcc aatgacctgg ctttaagatc tccacgccgt gaccggcatc cacccactgt
     1681 gggtccacgt atgtgccgct atgggcgagt tctctcgatg gagacgaggc gggcgctgag
     1741 ggtggtacct ggatgatgat gacatcgccc gtgtcgatga cgtggcctcc gataaaccca
     1801 acatcgccac cgaaccgcga ccgaaaatcg gagcggtcga aacgatctct gaaacgatgc
     1861 ggcctttgag ctggcccaag ctcaacgccg aaacctgagc gaaaattgcc tgtcaaacca
     1921 tgttggctgc gcaccgcggg agcgctgaat tttccggacc cacggaaaga gcgagacgcg
     1981 ctaaaagcca cctcatgaaa gaggaggctc atctcaatag caagcacaaa accgtagatc
     2041 atgcttttct tatgcgccgc ctgccccata ccttttctct aatccaaatt aatagacaag
     2101 aaaaggacgc ctaggccctt gccaatagag cgcatgatgt cgagcacatc gtctcgcgta
     2161 accctcaggc tgcccgtcaa agacacgcgc gatgagctcg tgactacgtg catcatggtc
     2221 gaatatccaa gcaaccaagc cgacagggta atcgagcagt tattttggtg gtctttgcca
     2281 gttcttcaat ctgccgtgcg ccacgagaac gggcccccag taccttgtcc atcttgatcc
     2341 tttttgattg cactcccgat catgctctct cctgaatggt cgaaagagtg agtcgcattt
     2401 gtcagggaat cggagatatc aatttttcgg tcaatcgcat ggaagtatat ctcggcgcag
     2461 gcgcgggcgt tgaaaactat tcacggcctg ctggagatcg atgcgatccc agttcttcct
     2521 gctgccggat attacagcta tttaactttg aactggagct tttaagaacg gagagcgatg
     2581 aaatgcaagc ttcagtgtct atagcgacga gggatctttg atgtaactac ttcatcagcc
     2641 ccgcagcgcg caggtactgg aggtcgacga accgttcagc ggcaggtagc ggtgcatgca
     2701 attggccggc ttccgccata actcgaatca cctgatcgaa gcctttcatg tcgatttccg
     2761 cctgttgggg aatgacgccg cggtccggat caaaataaag ggacaatgtc tggcgcgcga
     2821 tcgcttcgct cgatcccgtc gtatcgatga ccatcttgac tacttcatcg cggttgcccg
     2881 ggtcccgcat gaagcgaaac gccgcggcga ggccgcggac gtagcgaagc agcgcgtcct
     2941 tgttcttctc cgcccaggac tttcttaccg cgctgacgat aaactgaaaa ttaggcatgg
     3001 catcggtagt aacacccaaa cgccggtagc cctttcccat cagcagcagg tcctcgggct
     3061 ggcccagcgg tacggcatcg cattcgcctt tgtgcaggca ttccgaccgc gccggcgtgc
     3121 cgaccagctc tctaagtttg aagtcagact ctgtaatacc gttcatggcc atgagcttcc
     3181 gcgtggaaat agaaatcgta tcgacggcga gcgaaagacc cacggttttc cccttcaact
     3241 ctgcgaaaga tttaatctcc ggcttgacga tcagactgta gatcggtgtc gccgtctcgc
     3301 cggcgatggc gacaccatcc gaacctttga gcacggactg aatcaggtag ggtgtagcgg
     3361 tgcgggtgat gtccacttta ccgtcgttga gagcgaccat catgttgtcg gcgccgcctt
     3421 cgatcggcat gagattgagc tggatgcctt cgcgctcgaa taaacctttg cgttggccga
     3481 ttacgaactg aatagcggat acagtcttga tggacgaggg aatttggcca tagcgaagcg
     3541 tgacgctttg tgcaggagaa gacgatgctg ggaaaacggc ccagacgcaa atgccgagta
     3601 ccgaaagcat cttgctagtc atcttagatc ctccttacaa gagatgtggt gtggacaaaa
     3661 atatcacaag cgccacgaaa gcacagaaaa attgctgaac caaatattca ttatgcacga
     3721 gtcagagaac ggattagacg tacatgctgc gagtagctgg tgaagcttgt aggggcaatt
     3781 ctaactgcaa ataggctcta catattcgaa aaaaacgctt ctgtgcaaga gaattgctaa
     3841 agttgcggca gttccatttg gctgtgacta gactccctaa gcgctttctc gattgattca
     3901 tcctcattgc ttatcagaag ttgtccgggg aatagtcttc ccctcgtgca cacgtatcgc
     3961 accacaagtg acgtcactcg gctgtccttc cctgaccact gaatacggac gactagatcc
     4021 ggaacgtacg gtgcgtgctt tccgggaaca acgcatcgat cgagagacgc tcgggtgtga
     4081 gcccttgctc gtgcatgtag cgggcgaagg tctccagggt gtggcggttg gactccaggc
     4141 catagggcca gaagtcgtcg cccatcagat ccagcgtctc gtaggcgtgg tcgaccagaa
     4201 acggcagcga gaccggcaag gcgctggtct gggtgaggtc ttgcaaggcg agcgccttgg
     4261 cttcttcgaa agccttggcc aaggtgacgg ccacccacgg gttctcatcg agcacttcgc
     4321 gcttgatcac ggcggtgtgc atgatcggga agaggcgggt gcggcgaaaa tagtcacgct
     4381 ccacctgcca gggattgtcg aacaggcgcc gcaccgggcc gccgccgcgg cggaaggttg
     4441 acggcgtgcg cgcggtgacc agcgcgtcaa gctcgcccgc ggccagcatc tgcgccaggg
     4501 agcggtcgtt gggcgcaaag ccgagcttca cgccgggcgg ctcggccggc tcgaacggca
     4561 gccggccggg atcttcaagg ccaccctgga cccactcgac gtcttcaggc cgtatgccgt
     4621 agtcgtccgc cagaaagccg cgcacccaca ccgcggctgt catctggtat tcgggcacgc
     4681 cgacacgctt accgcgcaga tcctgcggac cctcaatgcc cgcaccggca ttaacgaaca
     4741 gccccgaatg gcggaagaag cgcgacagga aaaccgggat ggcgaccagg tcatccgcgc
     4801 ctcggccgcg gcggacgaag tagccgccca gcgagaactc cgacgcatcg aattcacggt
     4861 actgggccat gcgccagaaa atttcttcta cccgcaacgt caggtaggtc agcgcgatgc
     4921 cttcggggcg cacccgcccg tcgcgcagcg cctcggtacg gtcgtagtgt ccggcagcca
     4981 gggtcagttt catcatcacg tcgtataatg tagagagaat ttaaaccgct tttttctgac
     5041 tcgagatata tcgtcggcgc gctgaacgtg tcaaacggtg aaccataatt ggcaggcgtt
     5101 ctaccacatc agatacgggt ttgcgtacat ccttcctcag acggaactta ccggctgcct
     5161 acgctcgcgc cgcgatcctt accgccttgt taagctgaac tccaaatccc tctgcgatcg
     5221 gctgataacg gtgagcccca gtgtcgtacg atgacctgat tgcatcgtct cgatggcgaa
     5281 aatatccttc cgttaattgc tgggggcatg gaacgtgatt aaaaagactc caggaagata
     5341 ttctcagtgg ttgcgccatg gccgcattta atgaaaggta ggcgggacta ccgaaaaata
     5401 aaagagttta ccccgattct tcgaatagaa ggcggacagt acagccgtgc tcggagtggt
     5461 tcgcgtttaa ccaaaaagac gcttgcgaga tgcgcaaaat tcgcgagtca ccctacggct
     5521 gctgagtcag gtcgagaagc gaggtgatct tcgtagtctc ttttccgaac ttgatcggaa
     5581 gatcgacgag ccctttgacc tcgaacggag gcgcggcaag atcaatcccc cggtatgctc
     5641 cgatgggatg tcctggttgt cgctgctgcg gtcattcgtc aggatgaccc atttgtactt
     5701 gcccttccgc agattattct taatgaactt gtgaattaga tcgttggaga gatgctgcag
     5761 gacgtccttg gatatgagca ggtccgcggc ggggagttcc tccgtgatgt cgccgacctg
     5821 aaacttgacc ttgcctgtct catacttctt gcgaacgggc acgataacat cggaggcgat
     5881 gtcgatcccg aggtagctcg catcacccca atcgattgcg ctcgagtagc tccagtcccc
     5941 gcagccagcg tcgatgaccg agtttacttc gtgcttctta atgaagtctt cgatgtatgc
     6001 gcgatactcc cgggtgatct ccaaagtcga tcccgaaccg ctcgttccct tgccggcggc
     6061 gtccgtggcc catcgacccg tagcatagat gcgattgaaa gtgtcagtca acgacggctg
     6121 tcggttaatg gcattgattg cgaagtagga aaccgccagc agagcagcca gggcgatggc
     6181 aatgggaacg ggcttcttca tcaagtctcc ttggttcctg ccgccgctaa tagctcgtcg
     6241 cacttccggc aaacggcaag cagagcattg ttcattgaac aggcaaaatc acgaatgatc
     6301 ttattggccg tgacatcaat gatcgtaaag tttccgacga agtctcagac agccattggc
     6361 caggagccgt tgtgatgacc gcgaaagacc gtccggctcc ctcctggtca acgcattaat
     6421 aacgagttta gttaatgccg ctttgaatgt ctctttatag ctttgtagcg gatcaactga
     6481 cgctcattag tgcatgcgcg gacacatcga aacaatcaag cttacgccgc tacctcccag
     6541 cgcggtgctc cacttgctcc acaacgtaat ctgccgatgc ccttttgacc atcgaagcgg
     6601 caaagcgatg ggtggacgcg ctggcgtttc aaacaaaact cgttccgaca ttgtcatttc
     6661 gtcacgttct ctgatattgg ggcaccggct gatggaacag cgacccctca ggaagaaaaa
     6721 ctgaggggtt cgtgccggcc tagcgtcgta cagaagcgat gcaaaaattg tcgagtgggg
     6781 cgatgaagcc gcgtttaccg attgcgaata gagccgcgct gattgaccgt gctggcaatg
     6841 gctaccctaa tcgcttcact tccccatcac accgcgatac gccgcttcga tattgttccc
     6901 caggcgcgcc tggccttccc agtcctcggt accgggcatc ttcaactctt tttttatttc
     6961 atcgagggat ttgccttgtt ggaccatctg ccgtacgccg tccatcagtc tgttgtagta
     7021 gctttccatg tcgtcgagaa tcttcgtcgt tcccggatcg ccatggcccg gaatcacgac
     7081 ctcaggattg agggctttca tcatcttgat cgcgctcagc gtgtccggga tgctgacaga
     7141 cggtcgatga ttgccgaagc gcttcactcc aacggaagcc gctgtaaaca gcacgcgttc
     7201 ctgaggcagc caaatcgccg tgtcggcttc gctatggata ttcttcagat aatagaggtc
     7261 cagcgcgcgc tcgcctacct tcagagataa tttatcgcga tattcgatat gcggcgtaac
     7321 caggcgaaag ccgttgaagg ccgcccgcat ctcggtcgag gtcgacatca atttctcgat
     7381 acgcgccggg ctaaacgact cggaggctct catcgaatca gctgagccgg catgaccgat
     7441 cactacggcc gggggtgaga agacaaagtc gcccatggcg tgatcagggt ggggctctgt
     7501 atgaacgatg tagcgcaccg gttggggtgt caatttcttg accgccgcca tgacgacgtg
     7561 cgaatctttc ggactctgcc ccgtatcgat caacaccacc ccctcttggc tcagaatgat
     7621 cgtggaattc gcttcgttga gcaccgcggc ataaacgtag ataccttccc tgaccttttt
     7681 aaactgtgcg ccgagatcct gcgcgatagc acggctcata acaaagaaac aaagaaacag
     7741 catcaaacag ccaagaatct tgcttttcat ccgattctcc ttggtgttcc gggtttaacc
     7801 acccttcacc actttgtagg cggcttccac attggtcgga aaccgctcct tggtcgccca
     7861 atgatcatac tccggcatct ttagctctcg cttaatctcg tcgagcgatt ttccctcggc
     7921 aaccattttg ccgacgcgct cctccagcag cgcgtaatat ttttctgtat cctcgaagat
     7981 tttcaccgtt ccgggagttc catgtccggg cacgacgatt tcaggattca atgccttcat
     8041 cattttcgat gcggccagaa tatccggaat cgtcacgaac ggacgaagaa tgttgaattg
     8101 atcgacgacg atgcccgatg ccgaaaaaag cacccgctct ccgggtagcc agacggccgt
     8161 gtcggcctca ctgtgaacgc ccttcaagta cagcagctcg aagctccgtt cgccgagatt
     8221 caaagtcatt ttcccccggt actccacttg gggaggcaca aatcgataac cgtccaaggc
     8281 cttgcgcatc tcaggcgaga ctccggcaag cttttcgata cgccccggcg tttctttttc
     8341 gcgattgatc atggattggg tcgcgccttc gtgcgcgatc acggtcgcag gcggagaaaa
     8401 tacgaagtga ccggtagtat ggtcgggatg aggttctgtg tcgattaaaa accgaatcgg
     8461 cagcgggctg atcttcttca ccgcatcgag gatgcttctc gaatcggtgg gattgtgacc
     8521 gctgtcaatc agcacaacgc cttcttgagt gaccacaata ccgcaattgg aattgaagtt
     8581 gctccccaca tagacgtaga tgccatcctt aatttttttc aaattgggcg gcagttcctg
     8641 actgtaggcg agaccggcgc acgcgacaga gaggaccgcg aggacgatga acgtaagctt
     8701 cggcttcata ccattcctcc ttttggcgag accggttaac agccgacgct aataagaaac
     8761 caaacgcgat gtcaatccgc tatttagcgt atattgcgtg tccgcgacga gagcgggcgg
     8821 ttgaaaaaaa cccggccttc aacattgtga ctacggcggg agaaccccgg acgagctcgc
     8881 ttcgcttcgc aaaatttgcg caacactgga ataaaaattc tacgagcctc ttgaacgtag
     8941 cgacttgcga gtgtcagata gccggctttg aatgccacgc gcggtattcc tatttgcggc
     9001 ctatgaatag taaccctggt aagaaggtcc aattatggta attgtctccg tcacgcggct
     9061 ccgcctgcgc tccttacctt tttcccggat tcctttggta cacccggcga tcggtcacac
     9121 aggcaaaacg gaccccgggc aatcttgcgt ccgagttaga aaatcaaaag ggcttgcgtt
     9181 ctggacattg gagatgtggc gggacaatca agcgatccga acctttgagc ccgcttcgcc
     9241 gcacggcgga gcaatgcaga agttgccgca ctggtgcgac gaagccgcct ttgccgattg
     9301 ggaacaagat acggctgatt ggccgccctg ggaaaccgcc aggggaaaat tggctgctac
     9361 gggacgattg gttgacgtgc tgcttccatc ggggcaacac cgggccgcga ggattgaaac
     9421 tacttagctt gctggcgaat tttgaacacg ttcaagccgt tcaaaacgtt caattgc
//
LOCUS       DASUVS010000288         4548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_553963, whole genome shotgun sequence.
ACCESSION   DASUVS010000288 DASUVS010000000
VERSION     DASUVS010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553963"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VF182_15810"
     CDS             complement(<1..939)
                     /locus_tag="VF182_15810"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6438603.1"
                     /translation="MINDLREFLNLLEAKKDLVHISRPTSPRFEIAAGIRKTSDIEGP
                     ALLFDNVVGHSMPVVGGLYAVRRRAIWGLETTPDKIHSKIMEGLKNHIPPRLVKDGPC
                     KEVILTGEDADFSRLPICTHNSKDAGPFITIGLGFIRHPNYGNNVSISRMQIYDGKTM
                     GCRSVPPQHLGVYFREFEAKGKAMEVAFTIGNDPYVTLCSQVSGSIFMDELEIAGGWR
                     GEPVDVVQCETIDVQVPATSEIVLEGELTAGERRTEGPFGEFPGYYQAVTEQPVFKLK
                     AITHRRNPIYITALTGPPTTDNHVMREIVLEAVLYDR"
     gene            complement(959..1435)
                     /locus_tag="VF182_15815"
     CDS             complement(959..1435)
                     /locus_tag="VF182_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6438604.1"
                     /translation="MKIEIRTTVNGREMEAVVKPNQTVLEWLRDDLRLRGSVEGCGVG
                     VCGSCTVLVDGRPVSSCLMLATNIEGKQLTTIEGLATGDDLDPIQQAFLDNQAFQCGY
                     CTPGMIMAIKGLLNSNPRPTEEEARDYLSGNICRCGTYSEVLAAVKSLSEGNGTKQ"
     gene            complement(1432..3750)
                     /locus_tag="VF182_15820"
     CDS             complement(1432..3750)
                     /locus_tag="VF182_15820"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6438605.1"
                     /translation="MSSVGMNVSRADGPAKIRGAAQYAADVELPGMLYVKALRSAYPH
                     AKLLRVDASKAKKLPGVVAVLTSDDLKGKNAYFGPVVKDQPVLAIDRVRYVGEVIAAV
                     AAEERDIAEEALDLIEVEYEPLPAVYDLLEAMKPEAPVLHADRIKAHSFPAKSGFRLQ
                     DEGNVLSRYHVDDGDVAVGFSESDEIFEGVYTSPKIQHAHIEPHATVAYWEPSGKLVL
                     YTSTQTPSSIRTQLAELLALPQSMVRVIVPFVGGGYGAKTHPRLEPLAAFIARKARRP
                     VQWVLTREEVFLTAHCQAAVVQVKTGVKKDGTILARQVEAVYDIGAYALTSTNTGRNG
                     GEVSGGPYRIKHQHLTTYCVYTNTPPTGPYRGFGVPQVCWAYESQMDDIARRLGIDPL
                     ELRLKNLVREGDPFVTGHNLVSVGISDCAKRAAEAVGWQQDAEQAPRALSGKVRGKGM
                     AVMIKTTMTPSNSSALVRLNADGSAVLLTSSVEIGQGTLTSLAQIVAEELGLPVDRVG
                     VTFPDTDVTPFDQSTSSSRTVFTMGGAARQAAHQIRQQLLEIGARVFEANVEDLQLVD
                     GFLEVKGVPDKRRSIPQLFQARYGAAVGNMVGSFDNQTSGGLDPTTGKGKGSVFYFLS
                     ACAAEVEVDTDTGKVTVQQVISAVDAGKAINPRQCHMQNEGSMIMSLGSTLFEEMVFD
                     NGQPINSTFLEYMPASMEDHPKKFQSLLIETPHPEGPYGAKGVGEAGLGPIEPAIGNA
                     LANALNGIRLKDLPLRPDRVLAAVQSAKEHGA"
     gene            complement(3760..>4548)
                     /locus_tag="VF182_15825"
     CDS             complement(3760..>4548)
                     /locus_tag="VF182_15825"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6438606.1"
                     /translation="EEDVKLIAGGTALLILIKQGIFVPKTLINLKKIKGAGEITYNST
                     DGLRVGGLASIYDVESAPAVREHYPLLAEACHVVANIRIRNMATIGGNLAHADYQSDP
                     PAALVALDAKVELTNRHRVRSVKLADFFIGMYETGLERDELVTAVLVPPAPPSFKGTY
                     LKFTTRSSEDRPCAGVAAWVQHSNGNREAARVVIGAVSPVPVVVTAPELVNGEKLTSD
                     LIEDVASKAQAAVDPIDDLRGTADYKRQLVKVLVRRALTACATG"
BASE COUNT          943 a         1379 c         1198 g         1028 t
ORIGIN      
        1 gcggtcatac aacaccgctt cgagaacaat ctctctcatg acatggttat cggtggtcgg
       61 cgggccggtg agcgcggtaa tgtaaatcgg attgcgccgg tgcgtaatcg ctttcaactt
      121 aaacaccggc tgttcggtca ctgcttggta gtaccctgga aactcgccaa acggcccttc
      181 cgtgcggcgt tcgccggcag ttaattctcc ctcgagcacg atttcactgg tagcgggcac
      241 ctgaacgtcg atggtctcgc attgcacgac atcaacgggt tcgcccctcc agcctccggc
      301 gatttccagc tcgtccatga agatcgatcc ggaaacctga gagcataaag tcacgtaggg
      361 atcgttgccg atggtgaagg caacctccat ggctttcccc ttggcttcga actcgcgaaa
      421 gtaaacgccc aaatgctgcg gcggtacgct tctgcatccc atggttttgc cgtcgtaaat
      481 ctgcatccgg ctgatcgaca cattattgcc gtagttgggg tgacgaataa aaccgaggcc
      541 gatggtgata aatggaccgg catccttgct gttatgggta cagatcggca ggcgactgaa
      601 atccgcgtct tccccggtta agatcacctc tttgcaagga ccatctttca ccagacgtgg
      661 cgggatatgg ttcttgaggc cttccatgat cttgctgtga attttatccg gtgtggtctc
      721 aaggccccag atcgctcgcc gtcgaaccgc atagaggccg ccaacgaccg gcatcgaatg
      781 gccgacgaca ttatcgaaga gcaatgccgg cccctcgata tcggaggtct ttctgatgcc
      841 tgcagcgatc tcgaagcgcg gcgaagtcgg ccgactgata tggaccaggt ctttctttgc
      901 ttccagaaga ttgaggaact ctcgcaggtc attaatcatt gaacgtttgt ctccgactct
      961 attgcttggt gccgtttccc tcggacaagc ttttcaccgc tgcgaggact tctgaatacg
     1021 tgccgcagcg gcagatattt cccgacaggt aatcgcgcgc ttcttcctca gtcggccgag
     1081 gattgctatt cagcagtcct ttgatggcca taatcattcc cggcgtgcag tagccgcatt
     1141 gaaacgcctg gttgtcgaga aacgcctgct ggatgggatc gagatcatct cctgttgcca
     1201 aaccttcaat ggtcgtcagc tgcttgcctt cgatgttggt ggccagcatc aaacaagaac
     1261 taacggggcg gccatcgacc agcacggtgc aggatccaca gacgccgacg ccgcatcctt
     1321 caacgctgcc gcgcaggcgc aaatcatcgc gcaaccattc gagcacggtc tggttcggct
     1381 tcaccacggc ttccatttcg cggccgttaa ccgttgtgcg aatttcgatc ttcatgcgcc
     1441 atgctctttc gcagactgaa ccgccgccaa aactcgatcg ggccgcagcg gtaaatcttt
     1501 gaggcgtatt ccgttcaaag cgttggccaa tgcgttgcct atggccggct caatcggacc
     1561 gaggccggct tcgccaacgc ctttggcgcc gtacggccct tcagggtgcg gcgtttcgat
     1621 caacagagac tgaaacttct tgggatgatc ttccatcgat gccggcatgt attctaagaa
     1681 cgtcgagttg atcggctgcc cattatcgaa caccatctct tcaaacagcg tcgatcccag
     1741 cgacatgatc atcgaacctt cattttgcat gtggcattgc cgaggattga tggctttgcc
     1801 cgcatctact gcggatataa cttgttgcac ggttactttt cccgtgtcgg tgtccacttc
     1861 aacttcggcg gcgcaagccg atagaaaata gaagaccgaa ccttttcctt ttccggttgt
     1921 cggatccaac ccgcctgacg tctgattgtc gaaactgccg accatatttc cgacggccgc
     1981 tccatagcgc gcctgaaata attggggaat gctgcgccgc ttgtccggca ctcctttgac
     2041 ttccaagaaa ccatcgacca actgaagatc ctcgacgttg gcctcgaaaa cgcgggcgcc
     2101 gatctctaag agttgctggc ggatctgatg cgccgcctgc cgcgccgcgc cgcccatggt
     2161 gaataccgtg cggctcgaac tggtggactg atcgaatggg gtcacatccg tgtccggaaa
     2221 cgtcacgccc acacggtcaa cgggaagccc taattcctct gcgacgatct gcgccagcga
     2281 cgtgagtgtc ccctggccga tttcgacact gctggtcagc aacaccgccg agccgtcggc
     2341 attgagccga acgagcgctg aagaattcga cggcgtcatc gtggtcttga tcatgaccgc
     2401 catgcccttg ccacgcactt tgcctgaaag cgcgcgagga gcttgttcgg cgtcctgctg
     2461 ccaccccact gcttcggccg cgcgtttggc gcaatccgaa attcccacgg aaaccagatt
     2521 gtgccccgtg acgaacggat cgccttcccg caccagattt tttagccgca actccagcgg
     2581 atcgatgccc aatcgacggg caatatcatc catctgcgac tcatatgccc agcacacttg
     2641 cggcacgccg aaaccgcgat agggacccgt cggcggtgta ttggtgtaga cacagtaggt
     2701 cgtcaaatgc tgatgtttga ttcgataagg tccgccggat acttcgccgc cattccgtcc
     2761 ggtgttggtt gatgtcaatg cgtaggcgcc gatgtcgtag acggcttcga cctggcgcgc
     2821 gagaatggtt ccgtctttct tgactccggt tttgacttgc accacggctg cctggcaatg
     2881 ggccgtcaaa aacacttcct cccgcgtcaa cacccactgc accggacgac gcgccttgcg
     2941 cgctatgaac gccgccaatg gttccaagcg cggatgcgtt tttgcgccgt aaccgccacc
     3001 gacaaacggc acgatgactc gaaccatcga ctgcggcaga gccagcagtt cggccagctg
     3061 ggttcttatc gatgacgggg tttgcgtcga cgtatacaag accagttttc cggacggctc
     3121 ccagtaggcc accgtcgcat gaggctcgat atgggcatgc tgtatttttg gcgatgtata
     3181 cacaccctca aaaatttcat ccgactcgct aaagccgacc gcgacgtcac catcatcgac
     3241 gtgatagcgg ctaagcacat tgccttcgtc ttggagacgg aagccgcttt tcgccgggaa
     3301 cgagtgcgct ttgattcgat ccgcatggag cacgggcgct tccggcttca tcgcctcgag
     3361 cagatcataa acggccggca atggctcata ttcgacttcg attaaatcca gcgcctcctc
     3421 tgcgatatcc cgctcctctg cggccaccgc cgcgatcacc tcgccaacgt atctgacgcg
     3481 atcaatagcc agcaccggct gatcttttac caccggtcca aaataggcgt tcttcccttt
     3541 caaatcatcg ctggttaaca ccgccacgac ccctggcaac ttcttcgctt tactcgcatc
     3601 gacacgtaaa agcttggcat gcggataagc gctgcgcagc gcctttacgt acaacatccc
     3661 cggtagctca acgtccgcgg catactgcgc cgcgcctcta atcttggcag gaccatcagc
     3721 gcgtgaaacg ttcatcccta cactactcat ttgaattcct tatccggtcg cgcaggcggt
     3781 cagcgcgcga cgaaccagaa ccttgacaag ctgacgtttg taatccgccg taccccgcaa
     3841 atcatcgatg ggatccacag ccgcttgggc cttggaagcg acatcctcga tcaaatccga
     3901 cgtcagtttt tcaccgttga caagctccgg agcggtgacg acaaccggaa ccggcgaaac
     3961 cgcaccgatg acaactcgtg ccgcttcgcg atttccgttc gagtgttgca cccacgcggc
     4021 gacgcctgcg caaggccgat cctcggagga gcgagtggta aattttaagt aagtcccctt
     4081 gaagctgggc ggcgctggcg gcaccagcac ggcggtgact agttcatccc gctccaggcc
     4141 agtctcgtac ataccgataa aaaagtcggc taacttgacg cttcgaactc tatggcggtt
     4201 cgtcaactcg accttggcat ccaacgcaac cagtgccgcc ggagggtcag actgatagtc
     4261 cgcatgcgcg aggttaccgc cgatagtcgc catgttacgg attcggatgt tggccaccac
     4321 atggcaggct tcggccagca gcggatagtg ttcacgcact gccggcgcgc tttcaacatc
     4381 gtaaatgctc gcgaggccgc cgacccgcaa accgtctgtg gagttgtagg tgatttctcc
     4441 ggcgccttta atctttttca aattgattaa agttttcgga acgaagatgc cctgcttgat
     4501 caagatcagc agagcagtgc cgccggcaat gagcttgacg tcctcttc
//
LOCUS       DASUVS010000289         2776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1428909, whole genome shotgun sequence.
ACCESSION   DASUVS010000289 DASUVS010000000
VERSION     DASUVS010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428909"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VF182_15830"
     CDS             <1..422
                     /locus_tag="VF182_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEX6438607.1"
                     /translation="ESKGKENPYSVHADLQDCMQNLVGIIRTESELKKALDVIGQLKE
                     RARNLKVEGNRQYNPGWHLALDLHSLLSFAEAATIAAIERKESRGGHTRDDYPNTDPK
                     FAKINIVMRRKNGDLTASQEPLPEMPDELKSLLEEKK"
     gene            422..1177
                     /locus_tag="VF182_15835"
     CDS             422..1177
                     /locus_tag="VF182_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX6438608.1"
                     /translation="MAPQQVHMQVWRGDGSGGEFRDYRVEAGEGMVVLDVIHAIQAQH
                     ANDLAVRWNCKAGKCGSCSAEINGKPRLMCMTRMNTFQPGETITVSPIKTFPLIRDLV
                     TDVSYNYEKARQVPAFKPKAKEADGTRRMFQEDIDRVQEFHKCIECFLCQNVCHVIRD
                     HEENKKNFAGPRLFVRLAALEMHPLDTNDRIDLIKQKAGIGFCNITKCCTEVCPEHIH
                     ITDNAIIPLKERVVDRYFDPIAKLMGLFSEKKR"
     gene            complement(1227..1679)
                     /locus_tag="VF182_15840"
     CDS             complement(1227..1679)
                     /locus_tag="VF182_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438609.1"
                     /translation="MESFSLTMDGDTIIVRGRKHRNQAVENLSRRGFWQLFRSNRTAE
                     VDEWEAVELRYTPEQLSHMDDEGRSKRGNLGRPDAHSASQIVRAVGALVDQKQARLLS
                     VRKDEEKIEIEYELPLKGRATEDFTVATLYEYWVRMYLKRSNRLEQSK"
     gene            complement(1847..2371)
                     /locus_tag="VF182_15845"
     CDS             complement(1847..2371)
                     /locus_tag="VF182_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438610.1"
                     /translation="MADQKVYALLLRPVGQLLEALRIESFSLSVENEDLVVRDKTPGR
                     KQITPREKALLAEWQVTHTRPQDKEGALKLASGILEWRMTPADIDRLEREGRSRRRNP
                     NLTPDIHSVSQMLRVVGALVDHKNGRLLQLTKEDQTMTFEYELLNGKKVVEELGVPAL
                     YDLWVRIYRRQTPK"
BASE COUNT          672 a          736 c          685 g          683 t
ORIGIN      
        1 ttgaaagcaa gggcaaggaa aacccgtatt cggtgcatgc agatttacag gattgcatgc
       61 agaacctggt aggcatcatt cgaacggaat cggagttgaa gaaagctctc gatgtcatcg
      121 gacagcttaa agagcgagcg cgaaacttga aggtggaggg caatcggcaa tacaaccccg
      181 gctggcatct ggcgttggat cttcactcgc tcctgtcgtt tgcagaagcg gcaacgattg
      241 cggcaatcga gcgcaaagaa agtcgcggtg gacatacgcg ggatgactat cccaacaccg
      301 atccgaagtt cgccaaaatc aatatcgtta tgcgacgaaa gaacggcgat cttacagcgt
      361 cacaggagcc tcttccggag atgcccgatg agttgaaatc attgctcgag gagaaaaagt
      421 aatggcccca caacaagtgc atatgcaggt gtggcgaggc gatggcagcg gcggcgaatt
      481 tagagattac cgagtggagg ccggcgaagg catggtggtg ttagacgtca tccacgccat
      541 tcaggcccaa cacgcaaacg atctggcggt tcgctggaat tgcaaagcgg gaaaatgcgg
      601 ctcctgcagc gccgagatca acggcaagcc gcggctcatg tgcatgactc ggatgaacac
      661 gtttcagccc ggcgagacga ttacagtttc accgatcaag acgtttccgc tcattcgcga
      721 cttggtcacc gatgtctcgt acaattacga aaaggcacga caggtccctg cgtttaagcc
      781 gaaggcgaag gaagccgatg gcacccggcg catgtttcaa gaagacatcg atcgcgtaca
      841 agaatttcat aaatgcatag aatgtttttt atgccaaaac gtctgtcacg tcattcgcga
      901 tcacgaggag aacaagaaaa attttgccgg accgcggctc tttgtccgcc tagctgcttt
      961 ggaaatgcat ccgctggata ccaacgatcg tatcgatttg atcaaacaga aggctggaat
     1021 cggattctgt aatatcacca aatgctgtac ggaagtatgc cccgaacaca ttcacattac
     1081 cgacaacgcc atcattccgc tgaaagaacg cgttgtcgat cgttactttg atccgatcgc
     1141 aaaactgatg gggctgtttt ctgaaaagaa gagatgatct ggcatgtgat cgattcccgt
     1201 ccggcgccat aagccgaacc gtcgaattat ttgctctgct ctagacggtt gcttcgtttc
     1261 aaatacatgc gtacccagta ctcgtacagc gtcgctaccg taaagtcctc agttgccctg
     1321 cccttcaggg gcaattcgta ttcgatctcg attttttctt cgtccttgcg aaccgacagg
     1381 agcctcgctt gtttttgatc gacaagcgct cccacagcgc gaacgatctg cgacgccgaa
     1441 tgagcgtctg gtctgccgag attgccgcgc ttcgatcgtc cctcatcgtc catatgactg
     1501 agctgttcgg gcgtatagcg caattctacc gcctcccact cgtcgacctc ggcagttcgg
     1561 ttggaacgga acagctgcca aaatccccgc cgcgataagt tttcaaccgc ctggttccgg
     1621 tgcttgcgtc cgcgaactat aatggtatcg ccatccattg ttaatgaaaa cgattccatt
     1681 tggagtgact ccagcgcttg accaaccgga cgaagcaact gtgtataatt ttcaccgctt
     1741 ttcagtctgg aactcccgtc tgagtcagta tgaaagccat tttcttcgcc gagtcttcct
     1801 ggttcaatcc tttgaaagaa ccgtttaaat caatgcctct gcttagctat ttcggcgttt
     1861 gtctccggta gatgcggacc caaaggtcat aaagagccgg cactcccaat tcttcgacga
     1921 ctttcttgcc atttaagagc tcatactcga acgtcatcgt ttgatcttcc ttagtaagct
     1981 ggagaagtct tccgttctta tgatcgacga gcgcgccgac aacccgcagc atctgcgaga
     2041 ctgagtgaat atccggagtc agattggggt tgcgccggcg tgatcggccc tcgcgctcga
     2101 gccgatcaat atcagcgggg gtcatgcgcc actccagaat gccgctagct aatttcaacg
     2161 cgccttcctt gtcttgcgga cgagtatggg ttacctgcca ctccgcgaga agggcttttt
     2221 ccctaggggt gatctgcttg cgtcccggcg ttttgtcacg caccaccaag tcctcgttct
     2281 cgaccgacaa agaaaaggat tcaatgcgca aagcttcgag cagttgcccc acagggcgaa
     2341 gtaaaagagc gtaaactttt tggtcagcca tgggttctaa gtactatcct cattgacgtt
     2401 acccctaggg caaattgcgg tttcttttgc gagtcaaccg gtataagtct ttaccggtca
     2461 ctgagttctg tagctcgcgt tcttaaaata gctacaggtt aaccggcact cgaagccata
     2521 ttccgatagt ctcggtgcat taaagcttgc ttatctactt ttttaccttc tcctaagtca
     2581 agaaatatct gctcgctaat atcccttgcc cggcttagat ccgtcggaag ttactgccta
     2641 atcgttgagt accttgaatc tgcaatccgc ctttgctcga ggcgccacca ttctctagca
     2701 acttgtccaa cgcatcgtca ccgatcaaac cctccaattt gatcttaagg cgcagattat
     2761 tggcgctatc tgaatt
//
LOCUS       DASUVS010000290         4315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1648994, whole genome shotgun sequence.
ACCESSION   DASUVS010000290 DASUVS010000000
VERSION     DASUVS010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648994"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            522..2060
                     /locus_tag="VF182_15850"
     CDS             522..2060
                     /locus_tag="VF182_15850"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438611.1"
                     /translation="MPKTTNEISRRHFLIGSAALGAGVITAPYLFRNAFAASRDRVTI
                     YQNTVADSINPYNQSSGSIYGNWQHVIEPLVELDYTKKAWVGVLADSWNFKGKQWVFK
                     LKQGIKFHNGAPLTSKDVVFSVERMRDQKGGSLQASNFNDVTEIQTPDDQTVIFVTKQ
                     PLAIFLDRLRNRFIVSKVAGDKYGDQLYQNLVGTGPYKFVSFQRGGNLVFTRNDDYWG
                     GKAAIKDVVFRKVTEDAARLAALEAGQADFINAIPVHEVGRLQRHPRIRIDKIEGLEM
                     YFLMMNVAYKPFDNKLVRQAVNHCVDAAAIVKNIFDGIGYPIDGPVGPHVIGSDPKLK
                     RYPYDPNKSKALLAQAGYADGCDVQLYYSAGRYPKDREVCQAVAAQMVKGGFRVELVS
                     QEWALFWGQDGVNGGKLPFYYNNRGSLTDADTFYDQYFRTGTTKRCNYSNPSFDKIID
                     EEQMSPDTKKRIALLQQAGKILMDDAPFVPLYNLASIYGAAKNLAWQMRPDEKVLGWD
                     MKIV"
     gene            2395..2574
                     /locus_tag="VF182_15855"
     CDS             2395..2574
                     /locus_tag="VF182_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438612.1"
                     /translation="MPVFSFLLKRRLLQGLLGGANRSVQAGRARRRLGGSFRKAAFAG
                     ITAMLVQRMLRRRSF"
     gene            2711..3427
                     /locus_tag="VF182_15860"
     CDS             2711..3427
                     /locus_tag="VF182_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HEX6438613.1"
                     /translation="MSVEQAKPAAGPTTRQNKSLLLALLVLTFNTGLIDAVSYIGLGR
                     VFVANMTGNVVLLAFAAAGISGLSITRAVSSLGGFLIGAIIGGKLGTSLAAGSRRRLI
                     LSAGILEALLIFTAAVSAIGFDIRSSAPVHQLYAMIVLTALSMGLRNATVRQLGVADI
                     TTTVLTLTLTGLAADSSVAGGSNPRPARRIFSILALFVGALSGAYLLRYGVALPLFVT
                     GACVLLTTAAYTAIAATAKE"
     gene            3795..4166
                     /locus_tag="VF182_15865"
     CDS             3795..4166
                     /locus_tag="VF182_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438614.1"
                     /translation="MFKLRYWFDADCEGHGESRLEAEIRKRCAHIQERTSQRKSAEDG
                     SAHRYRPYGLIFGVVFVVLSSGPFVAVKFFEALSIMTDVNGDFALLTGLWAVLTLPFA
                     AMIYLIGAMMDAERVVKWFNL"
BASE COUNT          978 a         1169 c         1132 g         1036 t
ORIGIN      
        1 ggttggcacc ccgttacgaa caatctaatc taaacttttc gcggcgtgtg ctccagacag
       61 gttgatattt tttatccggg aagccgccgc ccgccagcgt taacctgcgt ctgttgccgc
      121 aaaaacttat ggtctcacaa ctcgattcaa cgctcgcgac gcagaagctt ttcggtcagc
      181 ggaacggccc gttgccagtg cgcaattaaa gcgctgagcg cagccgtcgt cggcaacttg
      241 gactcgtgcc ggcaacaacc cagcatagct tcgaactatt cgcgcaggtt aaaaaatgtt
      301 cgacgagctc gggctggcat tatttttttt cgatcctttg caggtttacg ctcgaacctt
      361 atctagactc ggcgttatga ttccgctaca gtccgaagcg tcgggccgcc taggagcaga
      421 atcatggaca cgctgtcgtg gtggcatccg gctttgccgc tgcgcgctgc atcctgcaca
      481 tgccgaattt tgtggaacga ccgcgatcaa gggaggatcc catgcctaag actaccaatg
      541 aaataagccg gcgacatttt ttgataggat cggccgcgct tggcgcaggc gtcatcaccg
      601 cgccatacct gtttcgcaat gctttcgccg cttccagaga ccgcgtgacc atctaccaaa
      661 acaccgttgc cgattcgatc aatccttaca atcaaagttc tggttcgatt tatggcaact
      721 ggcagcacgt catcgagcca ttagtggaac tggattatac aaagaaggct tgggttggcg
      781 tccttgccga ctcgtggaat ttcaagggaa agcaatgggt gttcaaactc aagcaaggaa
      841 tcaaatttca caatggcgcg ccgctgacct cgaaagacgt cgtgttctcg gtcgaaagga
      901 tgcgcgacca aaaaggcgga agcctgcaag catcgaactt caatgacgtc actgaaattc
      961 aaacgcccga tgatcagact gtcatcttcg tgaccaagca gcctctggcc atttttttgg
     1021 ataggttgag aaatcgtttt atcgtcagca aggttgctgg agacaagtat ggcgatcagc
     1081 tttatcagaa tctcgtcggc accgggccct ataaatttgt cagtttccaa cgcggcggaa
     1141 atcttgtttt cacacggaat gacgattact ggggcggcaa agcggcgatc aaagacgttg
     1201 tttttcgcaa ggtcaccgaa gatgcggcgc gcttggcagc attggaggcg ggccaggccg
     1261 atttcatcaa cgccattccc gtccacgaag tcggccgact acagcgtcat ccgcgcatta
     1321 ggatcgacaa gatcgaaggt ttggagatgt attttcttat gatgaacgtc gcctacaaac
     1381 cttttgacaa taagctggtc cggcaggctg tcaatcactg cgtcgatgca gcggcgattg
     1441 tcaaaaacat ctttgacgga atcggttatc ccatcgatgg gccggtcggg ccgcacgtca
     1501 tcggctctga tccgaaactc aaacgctacc catacgatcc caacaagtct aaggcactct
     1561 tggcgcaagc cggatatgcc gatggctgtg acgtgcaact ctattattcc gccggccgtt
     1621 atccgaaaga ccgagaagtc tgccaggcag tggcggctca gatggtcaaa ggcggcttca
     1681 gagtggagct cgtttctcag gagtgggctt tgttttgggg acaggacggg gtcaatggcg
     1741 gcaagcttcc gttctattat aacaaccgcg gatctttgac cgatgcggat accttttacg
     1801 atcagtattt tcgcaccggt acgactaagc ggtgcaatta cagcaacccg tcattcgata
     1861 agatcatcga tgaagaacaa atgagccccg acactaagaa acggatcgcg ttgctgcaac
     1921 aggccggaaa gattctgatg gacgatgctc cattcgtgcc gctctacaat ctcgcatcca
     1981 tctacggcgc agcaaagaat ctggcctggc agatgagacc cgatgagaag gtgctcggct
     2041 gggatatgaa gatcgtatag gcgggcaggc aaccgcgatg acacgggata accgctcgtt
     2101 cagcttcccc tacccttcaa atgcgcttcg tcgcggctta gccgcacttc aaatgatcac
     2161 ttgatcttcc agaaccatcg gtgccgcgta actcttgggt tgcattaagg ctcagtcaag
     2221 attctcgtcg cctcgtagaa ttcaatctgt cccgcggaca gcgtccatgg aatgccgctg
     2281 tcgctttggt aatacactta atcgaattca tccaatactg cgacccgaca aaatcatggc
     2341 atcctccttg cggacccatg cgggtcagat cgtttcgagt aaggagctag agaaatgcca
     2401 gtcttttctt ttttgctgaa gcgccgactt cttcagggtc tcttgggtgg agcgaaccgc
     2461 agtgttcaag cgggacgggc tcgccgccgt ttgggcggct cgtttcgcaa agcagccttc
     2521 gccggtatca ccgcgatgct tgttcagcga atgctgcggc gcagatcgtt ttagtcttcc
     2581 tttgtgcagc tccgacgcgc cgcgatcagc actggagttg cagaagctcg ttgtcttccg
     2641 tggcacacga attccaaacg taagccggaa gtgaaccgat ccgcccgtga ctctgcggtg
     2701 tgaaacagtt atgtcggtag aacaggcgaa gccggcggcc ggtcctacaa cacgtcagaa
     2761 taaatcgtta ctcttggcgt tgttggtgct aaccttcaac accggcctta tcgatgccgt
     2821 cagctatatc ggccttggcc gggtattcgt cgccaacatg accggtaacg tggtactttt
     2881 ggcattcgcc gcggctggaa tttccggact atcgatcaca cgcgctgtat cgtctcttgg
     2941 cggcttcctg atcggcgcga taatcggcgg aaagctcggc accagcctgg cagccggcag
     3001 taggcgacgg ttgattctta gcgcaggaat tttagaagca ttgttgatct ttaccgcagc
     3061 cgtgagcgcg attggattcg acatacgctc atcggcgccg gtgcatcagc tgtatgcaat
     3121 gatcgtttta acagcattat cgatgggact tcgaaatgct accgtgcggc aactcggcgt
     3181 tgcagatatc acgaccacgg ttttgaccct cacgttgacc ggcttggccg cggactcatc
     3241 cgtagcagga ggcagtaacc cgcggccagc gcgccgcatt ttctcgatcc tagcgttgtt
     3301 cgtgggcgcg ctctcgggcg cttatctttt gcgctatgga gtagctctgc cgctcttcgt
     3361 caccggcgct tgcgtgctcc tcaccactgc ggcgtatacg gcaatcgctg cgacggcaaa
     3421 agagtagccc acgagaaaag ttgataaaaa aattcggcaa gagctaagcc acttggccaa
     3481 cggccatccc atgaacccgg ctcctgcgca ccccacacaa accgatagtc aagaatcgac
     3541 caacgatttc ccggcttgtt cctttttgct cgcctgacgt attgaagaac atttttcttg
     3601 attccgtagg ttaagttttc ttttatagac tgatggctta tcgtcggaat gggcggagcg
     3661 accgtggaac tccttggtat gttccttgct gattcgcccg cgccatggaa atgacatgga
     3721 accctagtca acactccgag ccaacaaacg cagtccaccg tgaggcagat tactcaaact
     3781 actcgcttcg agaattgttt aaactacgtt actggtttga tgccgattgc gagggccacg
     3841 gagagagtcg gctcgaagcc gaaattcgaa agcgatgcgc gcatatccaa gagcgaacca
     3901 gccagagaaa atctgccgaa gatggttcgg ctcatcgcta cagaccttat gggttgatct
     3961 tcggagtagt tttcgttgta ctttcgagcg gcccgttcgt cgcggtcaaa tttttcgagg
     4021 ccctgagcat catgactgac gtcaatggag actttgcgct gttaaccggt ctttgggcgg
     4081 tgctcacgct tccattcgcg gcaatgatct atttgatcgg cgccatgatg gatgcggagc
     4141 gggtcgtgaa atggttcaat ctgtaaccga aatcctgctg ccgcaatcca cttagagaga
     4201 ttccaagatt ctcccgattc tcccgtctaa accttcgcgt tcctcaacca gaaacttcgt
     4261 tccggactca tctaatctga gtctcatcca tactcattct ggcagccaac ccgag
//
LOCUS       DASUVS010000291         6132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_992168, whole genome shotgun sequence.
ACCESSION   DASUVS010000291 DASUVS010000000
VERSION     DASUVS010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6132
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992168"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VF182_15870"
     CDS             complement(<1..821)
                     /locus_tag="VF182_15870"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438615.1"
                     /translation="MVKIRRSISLMLLGALSLLGQTAYSAQSSDLKPEKNPLEVAIAA
                     TGPLYLPVLLANEAGYFSKRGLTVNISTLSATASAQALLSGQVDIYQGGTATIHANVG
                     GADLIYIAASVDRSTLILFGQKGLTSFENLRGKSVATTSVGAFGEIAMRKTAKEHGME
                     IGKDIKLLYHKGPPDALSTFLLGNAEGVIITPPQTEMARSKGFPVIIDFYEKGLKIIG
                     PGTGVARAFVKKNPNTLKIFLMGYLDGLKRTIDDPGYAQKILSQTSKITDPKLLE"
     gene            complement(858..1877)
                     /locus_tag="VF182_15875"
     CDS             complement(858..1877)
                     /locus_tag="VF182_15875"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6438616.1"
                     /translation="MNVRLWQCLTAMILLLPGSLCADDFYKGKTVRVIVGGSPGGGFD
                     IYTRAMARHMGKHIPGNPSLVVENMTGAGTFIAAKYIHSSAKPDGLTFGIFNGALILG
                     RVLGMKGVDIDMRELEYLGVPVQDSTVCALRKESGITNIDQWFASKTPMKLGGLSPGN
                     STSDVARMVGATLGLPVQLVEGYKGTNEIRLAADAGELHGACWAWETLKVAWQNAIPA
                     GDVNIVLQVTAKKLPDLPNVPMALDSAKTDEARLLLKAGAIDPAAIVRVYVTTPRIPK
                     DRLEILRKAFAATLTDPEFVAEAKKANLDINPLNGEEVKKTVDDLFKLSPAIISKLAN
                     ILAVK"
     gene            2102..3181
                     /locus_tag="VF182_15880"
     CDS             2102..3181
                     /locus_tag="VF182_15880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX6438617.1"
                     /translation="MDKNKGNELKVAPLEQQNKPIADLGWQLDEWLRGQSDYVNADQF
                     AGILRTLLKLAQDDAERGDLKILNRTLQELRHAFRIFAPYRHMRKVSIFGSTRVQESD
                     PYYALARHMGEELALADFMVITGAGPGIMQAGHQGAGREKSFGVNIRLPSVQDANPFI
                     RNDPKLMDFHFFFTRKLMFVKEADAVVIFPGGFGTHDELLESITLAQTGKTQLVPIIL
                     MDLPDGTYWSRWQEFLRDDVMSRGYINEREMTFFRIHTSAAAAVDEIKKFYRNFHSYR
                     FVRHELVIRVNHPPSATLIDRLNRDFKDIVIGEVRQTEPLSAESNDANTLHLHRLLMR
                     FNREDYARLRQMIDVINDDKSTSQA"
     gene            complement(3301..4155)
                     /locus_tag="VF182_15885"
     CDS             complement(3301..4155)
                     /locus_tag="VF182_15885"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6438618.1"
                     /translation="MTESLESFKSYIGKRETATDVVTASAMLKFAATLGQDNPPMEKG
                     APIPPGWYGAFFPASHRPSQMRTDGQASGGGIVPPIPLPRRRIGGTRVFFQEPLRIGD
                     EITRTTEIADIRIDDGPNGAMVTVIERNSISNSRGLAVVEERDMVMLSEARAEAAPSA
                     VPTVPAQATWLETIEPNPPLLFRFSAIRFNCHRIHYDRDYVTKVEKLPGLVVQSSLVS
                     QLLMEMCRKELPGRQLKSFDFKSARQIYDTGKFTIAGAPARDGREAELWALDSDGRVA
                     MTATAKFA"
     gene            complement(4398..5135)
                     /locus_tag="VF182_15890"
     CDS             complement(4398..5135)
                     /locus_tag="VF182_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015113587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HEX6438619.1"
                     /translation="MSQNGKSVSLVSRRQALILLGAFGGAMIAGRSNVYSASPRSNDG
                     KLPACVVTPKQTEGPFFIDEQLKRSDIRVDPSDDSVKAGVPLHLNFRVSAVGTAGCSP
                     LSGAIVDVWQCDAVGTYSAVNERDPRSAGKHFLRGYQVTDANGSTQFTTIYPGWYPGR
                     TVHIHFKVRADPQSGKAREITSQLYFDDALTDRIHAQQPYSSKGRRSVKNQQDGLFRS
                     GGGQLMLSPVESGEGYRATFDIGLQMA"
     gene            5465..>6132
                     /locus_tag="VF182_15895"
     CDS             5465..>6132
                     /locus_tag="VF182_15895"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HEX6438620.1"
                     /translation="MSDHKPYRLPLTVTPERYEIRLTPDLFAARFDGEVKIALQVHEP
                     VQQIRLNAAELVFHAVSIQQPAGAVLPGDATLDGDNEQAELDFHRTLAPGSWELRISF
                     SGVLNDKLHGFYRSTYKDNDGQEKPLASTQFESTDARRAFPCWDEPAFKAVFQPTLVV
                     DQALAAISNTRIVHETPVPGTAKKEVVFADSIRMSTYLVAFIVGEFESTEPVSLGHAP
                     LRVWA"
BASE COUNT         1324 a         1685 c         1693 g         1430 t
ORIGIN      
        1 tcctcgagaa gtttgggatc ggtgattttg ctggtttggc tgagaatttt ctgcgcgtag
       61 cccggatcat cgatggttcg cttcaggccg tccagatatc ccattagaaa tattttcaaa
      121 gtgtttggat tctttttcac aaaagctcgc gctactccgg tgccgggccc gatgatctta
      181 aggccctttt catagaaatc gatgatcact ggaaagcctt tgctgcgagc catctcggtc
      241 tgcggcgggg tgatgatgac gccctcggcg ttgcccagta gaaaagtcga gagcgcgtcc
      301 gggggcccct tatgatagag aagcttgatg tctttgccga tttccatgcc gtgctcttta
      361 gcggttttgc gcatggcaat ctcgccgaaa gctccgacgg aagtggtcgc tacggatttg
      421 ccgcggagat tttcgaatga cgtcaaacct ttctggccga acaatatgag cgtgctgcgg
      481 tcgacggatg cggcgatgta gatcaagtcc gcgccgccga cattggcgtg gattgtggcg
      541 gtgccgcctt ggtagatgtc cacctggccg gagaggagtg cctgcgccga cgcagtggcg
      601 ctcagcgtac tgatgttaac cgtcaagccg cgtttgctga aataccccgc ctcattggcc
      661 agtagaaccg gcagatagag cggcccggtt gccgcaatgg ctacttcaag cggattcttt
      721 tcgggtttca ggtcggaact ttgcgcgctg taggcggttt gacctaacag tgacagcgca
      781 cctagcagca tgagcgatat ggatcttcga attttcacca ttctcccggt tctccgtgca
      841 cattactgct tcctcggtta tttgaccgca agaatattag caagcttgga aattatggcc
      901 ggactgagct tgaacaggtc gtcaaccgtc ttcttcacct cttcgccatt gagcgggttg
      961 atgtcgagat tggccttttt agcttcggcg acgaattccg gatctgtcag ggttgcagcg
     1021 aaagctttgc gaagtatctc cagacgatct ttcggtatcc gtggcgtggt gacgtagaca
     1081 cggacgatcg ccgctggatc gatggcgccg gctttaagca atagccgcgc ttcatcggtc
     1141 tttgccgaat cgagcgccat tggcacattt ggcagatccg gcagtttttt cgcggtgacc
     1201 tgcaggacga tgttgacgtc tcctgctggg attgcgtttt gccaggcgac cttcagtgtt
     1261 tcccaggccc aacaagcgcc gtgcaactcg ccggcgtccg ccgccagtcg gatttcgttg
     1321 gttcctttgt agccttcgac gagttgtacc ggcagcccga gagtggcgcc caccatccgc
     1381 gccacatcgc tggtgctgtt gcccgggctc aagccgccta gcttcatcgg ggtcttcgag
     1441 gcaaaccatt gatcgatatt cgtaatgcca ctctctttac gcagcgcaca aaccgtgctg
     1501 tcctgcaccg gcacaccgag atactcgagt tctctcatgt cgatatcgac gcccttcatc
     1561 ccgaggacgc gccccaagat caaggcgccg ttgaagatgc cgaaagtgag cccgtcgggc
     1621 ttggcgctac tgtgaatata tttggcggcg atgaaggttc ctgcgccggt catattctcg
     1681 acgaccaatg aaggattccc cggaatgtgt ttacccatgt gccgcgccat cgccctggtg
     1741 taaatatcga agccgccgcc ggggctgccg ccgacgatga cccgaacggt cttgccttta
     1801 taaaagtcat cggcgcaaag cgagccagga agcaacagaa tcatcgcggt taaacattgc
     1861 caaagcctga cgttcataac gtagtcctcc aggtccggct gaatatcggt aaaatagcgc
     1921 tgttgtcaat gtctttttcg ctctccagcg aatttgtctg gatcgtcatg aagccaaaca
     1981 acgccgtcat taggaaatgc gtccggtaga acaatcgttt tactatcgga ggcgcgctcg
     2041 gttgtttata ataccttcat gcaggcgagg agtgacgttt tgggcggagt gtgaccgaac
     2101 catggacaag aacaaaggaa acgagttgaa ggtggcacca ttggagcagc aaaataaacc
     2161 gatagcggat ctcgggtggc aattggacga gtggctgcgc gggcagagcg actatgtcaa
     2221 cgcggatcag ttcgcgggga tactgcgtac cttgctgaaa ctggcgcagg acgacgccga
     2281 acgcggagat ttgaagattt taaatcggac actacaggag ctgcgtcacg cttttaggat
     2341 ttttgcgccc taccggcaca tgcgcaaggt cagcattttt ggttcgacac gggtgcagga
     2401 aagcgatccg tattatgcgc tggcgcgtca catgggcgaa gagctggcat tagccgattt
     2461 catggtgatc accggcgccg gccccggcat catgcaggcg ggacatcagg gagcggggag
     2521 agagaagagc tttggcgtca atatccggct gccttcggtg caagatgcca acccgtttat
     2581 ccgcaacgat ccaaaactta tggactttca ttttttcttc acgcgcaagc ttatgttcgt
     2641 caaagaagcc gacgctgttg taatctttcc cggtgggttt ggcacgcatg acgagctttt
     2701 ggagtcgatc accctggcgc aaaccggcaa aactcagctc gtgccgatca tcttgatgga
     2761 tttgcccgat ggcacctatt ggtcgcgctg gcaggagttt ctgcgtgatg acgtgatgag
     2821 ccgcggatat atcaacgagc gggagatgac cttcttccga atccacacca gcgcggcggc
     2881 cgcggttgac gagatcaaaa aattttaccg caactttcat tcttaccggt tcgttaggca
     2941 cgaactggtg attcgcgtca atcacccgcc ttcagccacc ttgatcgatc ggctcaatcg
     3001 cgatttcaag gacatcgtca tcggcgaggt ccgacagacc gagcctctct cggccgaaag
     3061 caacgacgcc aacacgcttc acctacaccg gctgctgatg cgcttcaatc gcgaagatta
     3121 cgcgcgcttg cgtcaaatga tcgatgtcat caatgatgac aaatcaacgt ctcaggcata
     3181 atgaattcac aatcccgatg agtgcttact tcgcgcagtt tgctgattcc tcggccatcg
     3241 ccgatcaaac cctggcgcgc tcatctagcc cagcgaaatc attgcattgc agctttgatc
     3301 ctacgcgaac tttgccgttg cggtcatagc gactcgaccg tccgagtcta acgcccacaa
     3361 ttcggcctca cggccatcgc gcgccggagc gccggcgatg gtaaacttgc cggtatcgta
     3421 aatctgccgc gcgctcttga aatcgaagga tttgagttgt cgccccggta actccttgcg
     3481 gcacatctcc atcagcagct gtgaaaccag agagctttgc accaccaggc cgggcagctt
     3541 ttcaaccttg gttacgtaat cacggtcata atggatgcga tgacaattga agcggatcgc
     3601 cgaaaagcga aacagcaaag gcgggtttgg ctcgatcgtt tccagccaag tcgcttgcgc
     3661 gggtaccgta ggaacagccg aaggagcggc ttccgctctg gcttcactaa gcatcaccat
     3721 gtcacgctct tcgacaacgg ccagcccgcg gctgttggag atgctgttgc gttcaatcac
     3781 ggtgaccatg gcgccattgg gcccatcgtc gatgcggata tcggcgattt cagtggttcg
     3841 ggtgatctcg tcaccgatcc gtaacggttc ctgaaaaaag acgcgtgtgc cgccaatgcg
     3901 ccggcgcggt aacggaatcg gcggaacgat cccgcctcct gaggcttggc cgtccgtgcg
     3961 catctgcgaa ggccgatgag aagcaggaaa gaatgcgcca taccatcccg gcggtatcgg
     4021 cgcgcctttt tccataggcg gattatcctg gcccaaagtg gcggcgaact tcaacatcgc
     4081 cgaggcagtc actacatctg tggcagtctc cctttttcca atatagcttt tgaagctctc
     4141 caacgactca gtcatccgat tacctcctga ctgttcttct tgggcggaga ctaatccgtc
     4201 atggtttaag ctgtcaagac aacggcgcgc cgcgacttga ggcgttcggg gacgaccgcc
     4261 gatggctgca aaaactctgg cgcaggctgc tcaaaatgaa gatagaaggc gcaaagcaca
     4321 aggatgaaag cggaaccagt tttcagcttt cataactcat ctttttccat cgcacacggg
     4381 tctttttgag cggcctgtta tgccatctga aggccgatgt cgaaagtggc tcggtagcct
     4441 tcgccgcttt caaccggtga cagcatcagt tgacctccgc cgctgcggaa caggccatcc
     4501 tgctggttct tgactgaccg tcgtccttta ctgctgtagg gttgctgagc atgtatgcga
     4561 tccgttagcg catcatcgaa gtaaagctgc gaggttattt cccgagcttt tcctgactga
     4621 ggatcggcgc gaaccttaaa atgaatgtgc accgttctgc ctggatacca tccggggtag
     4681 atcgttgtga attgtgtact gccgttagcg tcggtgactt gataacctcg cagaaaatgt
     4741 ttacctgcgc tccggggatc acgctcgttc acggccgaat aagttccgac ggcgtcacat
     4801 tgccatacat cgacgatcgc gccggacaac ggactacaac cagcggtgcc gactgcagag
     4861 acgcggaagt tcaagtggag cggcacgcct gctttgacac tatcatctga cgggtcaacg
     4921 cgtatatcgg agcgcttcag ctgttcgtcg atgaagaacg gtccttcggt ctgttttggc
     4981 gtaaccacgc aggccggcag cttgccgtcg tttgatctag gcgaggcgga atagacattg
     5041 gaacgcccgg caatcatcgc tcccccgaaa gcgccgagca gaatcagtgc ctggcggcgg
     5101 ctgactagcg aaactgattt gccgttttga ctcatggtcg aatgctaaca agaggacatg
     5161 attgccgcaa atactagcag actgctgaaa aaagagtcat attcttcgtt acgctcgatc
     5221 gtctcgcggc aacgtaatgc cgatttacgc ctccgattgc tcgcgcgcct cggctctaac
     5281 tctttttgaa cagtctgcaa cccgcgtttt catcaacctg ctagctctga catttcccag
     5341 tattcactaa aaccggatac atagcgctgt tgtcggcttg cggcacgatt gagttgcgac
     5401 gttgtggtag tgtagaaata aacccgggtg cacaactcgt ctcaagcaat cattaggagt
     5461 ttcgatgtca gatcacaaac cgtatcgctt gccgctaacc gttacaccgg aacgttatga
     5521 aattcgattg actcccgatc tcttcgccgc ccgctttgac ggcgaagtaa agatcgcgct
     5581 ccaggtgcac gagccggtgc aacagattcg cctcaacgct gccgagctgg tgtttcacgc
     5641 ggtatcgata cagcaacccg ctggtgcggt gctgccagga gacgcgactc tcgatggcga
     5701 caacgagcag gccgaattag acttccaccg gactctggcg ccgggaagct gggagttgcg
     5761 gatcagtttt tccggtgtgt tgaacgacaa gcttcacggt ttttaccgca gcacctacaa
     5821 agacaacgac ggtcaagaaa aaccgctggc atcgacgcaa ttcgaatcca ccgatgcgcg
     5881 ccgcgcgttt ccctgttggg acgagccggc gttcaaggcg gtgttccaac cgacattggt
     5941 ggtggatcaa gctttagccg ccatttccaa cacgcgcatc gtgcatgaaa cgcctgtgcc
     6001 tggaaccgca aagaaagaag ttgtctttgc cgatagcatt cgcatgtcta cgtacctggt
     6061 ggcgttcatc gtcggcgaat tcgaaagcac cgaacccgtc agcttaggcc atgcgccgct
     6121 tagggtgtgg gc
//
LOCUS       DASUVS010000292         5842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_556912, whole genome shotgun sequence.
ACCESSION   DASUVS010000292 DASUVS010000000
VERSION     DASUVS010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556912"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(438..728)
                     /locus_tag="VF182_15900"
     CDS             complement(438..728)
                     /locus_tag="VF182_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438621.1"
                     /translation="MFAVLTDAVECFQKYYGANTHRRRRLFAEAEAWILNPDDTWPYS
                     FENICEALNIEPSYLRIGLRQWRLDQSQKNSGLQIRKTLRYQNTVKQNRVCM"
     gene            complement(1343..2938)
                     /locus_tag="VF182_15905"
     CDS             complement(1343..2938)
                     /locus_tag="VF182_15905"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6438622.1"
                     /translation="MQHYDAILVGAGHNGLVTAAYLAKSGKKVLVLERRSMIGGVAAT
                     EEIFPGFKCSTGAHLAGSFSREISAELELEKHGLKLLSPDPLVFAPQLDGKSLLIGSD
                     PAKIVEEIARLSSADAEKFPSFCLLINKLAGFLRVLNGLPLPDGASSGSFHLSELIKI
                     AVKFHRLGKNEMHEFLRILPMSIADLLSEWFENESLKAAIAGSAIVNSFVGPRAQGTA
                     YVLLHYLSRQTGGPFRSAGFVYGGINQLPQALARAARGRGAEIRTDAEVAQILTRNGA
                     AKGVVLNNGDDISANVIISNADPKRTLLHLVEPTYLDPHFLLQIKNIRARGTVAKINF
                     ALDTLPWFKPKVGDSSAAELSGIVHIGPTLEYLERAADDAKYGRFSKAPFLEITIPSL
                     VDPTLAPAGKHVVSVWMQSAPYRLKESNWNDQRQTLGDRVTDILESYAPGFKNSILHR
                     QVLTPLDLEQTFGVTGGHLDHAELALDQIFFMRPVPGWAGYRTPIDNLYLCGSGTHPG
                     GGISGLPGYYAAKKILQSWPREN"
     gene            complement(2945..4534)
                     /locus_tag="VF182_15910"
     CDS             complement(2945..4534)
                     /locus_tag="VF182_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6438623.1"
                     /translation="MTTYDAIIIGGGHNGLAAAAYLAKAGKKVLVLERRHILGGAAVT
                     EELYPGFKYSVCSYVVSLLRPEVIQELELPRYDLEIIPLDSTFTPMPNGDYLARWGDH
                     AKTRREIARHSAVDAEAYEEYGKLMVQMAMAIKPMLGLIPPEPTSWNPKELYRLISLG
                     NHFRRLGDKLVYDLAKLMTMSSADFLDEWFETEVLKATMSASGIIGTFMGPRSPGTAY
                     VLLHHYMGELDGAFRSWGFARGGTGSISRVIAEAARHYGADIRTQSPVDQVLVKNSRA
                     VGVALGTGEEIKAKVIVSSVDPKRTFLKLIDARHLETEFLTKVNQFKIRGSSAKVNLS
                     LDALPNFTSLPGKGPHLSGAISISPSIDYIERAYDDAKYGDYSQRPYMDIIIPSTLDP
                     SMAPPGKHVMSVFVQYAPYHLKSGTWAEKREEFGDTVIDTISEYAPNLKNIILHRQIV
                     TPWDLEDEFGLTEGNIFQGELTLDQLFFLRPVPGWSKYRTPIKNLYMCGACTHPGGGV
                     MAASGRLAALEILKDVNRSFI"
     gene            4716..>5842
                     /locus_tag="VF182_15915"
     CDS             4716..>5842
                     /locus_tag="VF182_15915"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEX6438624.1"
                     /translation="MPIGTPFHSRTSALCTSQNWRTWSGYFAASSYDVVHDYEYHAIR
                     NTAGLIDISPLFKYEVRGKDALKLVNRVITRDVAKCAIGQAVYGCLCDGEGKVIQDGT
                     VFRLGEDQFRFHLAEPALRWLKLNAGGMSVSIDEVSEQIAALALQGPNALKILTQIVN
                     ADLGKLKFFRLMPADFAGVPVTVSRTGYTGDLGYEIWLPAADAERVWDVLADKGSVYG
                     LKAAGMLALDVARLEAGFILLEVDYIGADKALIDSQKYSPFEIGLGWTVDLTKDFFVG
                     SKQLRNDDAKVTPRQITGLEVDWRHYEELYQKAGLPPPVPAAAWRGSIPIYQDDLQVG
                     QATTGAWSPSVKKYIVLATIKSEHANPGTTLQMETTVEHER"
BASE COUNT         1335 a         1518 c         1638 g         1351 t
ORIGIN      
        1 ctcctctcag tccaccgacg ccgattgttt tggggaccgc gcgccgcccg ggtgtaaatg
       61 gggcaaggca gaatgaatgg ggccagccga aggtgctgct tggcgggagt ttcataatct
      121 gatttgcgct cgcaagtaca gaacacgcta ctaaaaagct tgctctcggc acggccgccg
      181 tgacgcaccc gtggcagtgc tttttgcaat tcggcggagc tcctaagttc tgtcgttgca
      241 atcggatgca gcaataaatc tcggcgtaat tggtgcgctt gctgagtcgg tcgagcagaa
      301 taatcatctt catcgagcgc ttgagaaaac ctgtcgctag ctcacgatac taatgcttga
      361 aggttttcag cccgagttga cgatgcgtac tctggacatc ttcatggcag tccgagacgc
      421 tgaaactctc cgtcctctta catgcacact cggttctgtt tcactgtgtt ttggtagcgc
      481 agtgtcttac ggatctgtaa gcctgagttt ttctgcgact gatcaagccg ccactgtctc
      541 aaaccaatcc ttaggtagct tggctcgatg ttcagtgctt cgcagatgtt ctcaaatgaa
      601 tacggccagg tgtcgtccgg gtttaaaatc caggcctcgg cttcggcaaa gagtctacgg
      661 cgccgatggg tgttagcccc ataatatttc tgaaagcact ctaccgcatc agtaagaacc
      721 gcaaacatga gtttctcttc cgggtgcaag tgtgatttct gccgaaacag ttggaaatac
      781 cggtgtgacg taaggatgtc gggctcgaag tacgaacccg acggattcga ccgtatagtt
      841 gtcgccgctc tattcattga tacctccgga tttaagcgcg ggagcaaaat ttgctccatt
      901 gaggcaagtg gcggagatac tgtacgttaa cggcggccag ttcaatcccg aaggatgcta
      961 aatgcgaaag gaaataagat ttttacactc aagacctcgt agcgtgtgat tattcttcac
     1021 acagcggctt gtcgtaacga attccgctgc gacaaagcat tgtgaaattc atgcattact
     1081 cttacaagcg gaacgatctc gctgcgcgca gtatgtgcac acggtcgact tcgagttggc
     1141 gtctggggtc gcgcagagca ttgaaggccc acaccgagtt tttactcggc atcgcgccgg
     1201 tgagttcttg tccgtactgg ggctcagccg cattcgggtt ggaggccgag ttgcggtctc
     1261 gcaaaggaag gcgaggtgtt tacctgacgc cgaggtctcg aagatagaac gaaaagaaat
     1321 gtagtgaatt acctgataag aatcagttct ctcgcggcca gctctgcagg attttttttg
     1381 cggcgtaata gccgggcaga cctgagattc cgccgccggg atgcgtcccg gaaccacaca
     1441 agtagagatt gtcaattggc gtgcggtatc ccgcccagcc cggaaccggg cgcataaaaa
     1501 agatctgatc gagggccagc tccgcgtggt caaggtgacc gccggtaacg ccgaatgttt
     1561 gttctagatc gagcggagtt agaacctgcc tgtgtaggat ggaattttta aatccgggcg
     1621 catagctttc caagatatcg gtcacgcggt cacccagcgt ctggcgctga tcgttccaat
     1681 tgctttcttt gaggcgatac ggcgcagatt gcatccacac cgacacaacg tgctttccag
     1741 ccggggccag cgttggatcg accaaggaag gaatcgttat ttctaagaag ggggccttgg
     1801 aaaatctgcc atatttggca tcgtcagcgg cgcgctcgag atactcaagg gtcgggccga
     1861 tgtgaacgat gccgcttagc tctgccgccg aagaatcgcc gaccttaggc ttgaaccaag
     1921 gcaacgtatc caacgcgaaa ttgattttgg caacggtgcc gcgcgcgcga atgtttttaa
     1981 tttgcagtaa gaagtgtgga tcaagatagg tcggctcgac caagtggagc agcgtcctct
     2041 tgggatcggc gttggagatg atcacgtttg cggaaatgtc atcgccgttg ttgagaacca
     2101 cgcccttggc ggcgccattt cgggtgagga tttgggcgac ttcggcgtcg gtgcgaattt
     2161 ccgcgccacg ccctcttgcc gctcgggcta gtgcctgggg caactgattg atgccgccat
     2221 aaacgaaccc ggcgctgcga aatgggccac cggtttgtcg cgacaaataa tgcagaagca
     2281 cgtaggccgt tccctgggcg cgcggaccga cgaaagaatt cacaatggcg ctcccggcta
     2341 tggcagcttt caagctttca ttttcaaacc attcgctcag caaatcggca atgctcatgg
     2401 gcaggatgcg caggaactca tgcatctcgt tcttaccgag gcgatggaat ttcacagcga
     2461 ttttaatcag ctccgagaga tgaaaactgc cggagcttgc gccatcgggt aacggcaggc
     2521 cgtttagcac tcgcaggaag cccgcgagtt tatttatgag caagcagaat gaaggaaatt
     2581 tttcagcatc cgcgctcgaa agccgggcga tctcttcgac gattttggcg ggatccgatc
     2641 ctatcagcag cgatttgccg tctaactgcg gcgcaaagac cagcggatcg ggagaaagaa
     2701 gcttcagtcc atgtttctct agttcgagtt cggcgctgat ctcgcgcgag aatgacccgg
     2761 cgaggtgagc gccggtggag cacttgaaac ctggaaatat ttcttccgtg gcggcgacac
     2821 ctccgatcat ggaccggcgc tctagaacca ataccttttt acctgacttg gctagataag
     2881 ccgccgtaac cagtccgtta tgtccggcgc cgacgaggat cgcgtcgtag tgctgcacgg
     2941 cggtttagat gaagctcctg ttaacgtctt tcaagatctc aagcgccgcc agccggcccg
     3001 atgccgccat cactcctcca cccggatgcg tgcaggcgcc gcacatgtag agattcttaa
     3061 tcggcgtgcg atatttgctc caacccggta ccggccttaa aaagaaaagt tggtcaaggg
     3121 ttagctcgcc ctgaaaaata ttgccctcgg tgaggccgaa ttcgtcttct agatcccagg
     3181 gggtgacgat ctgacgatgt aagataatat tttttaggtt aggagcgtac tcgctgattg
     3241 tgtctatcac ggtatcgccg aactcttcgc gcttctcggc ccaagtgcca ctcttcaaat
     3301 gataaggggc atactgcaca aagacggaca taacgtgttt acccggcggc gccattgaag
     3361 gatcgagagt cgagggaata atgatgtcca tgtaagggcg ctgcgagtag tcgccgtact
     3421 tagcatcatc gtaggcgcgc tcgatatagt cgatgctggg gctgatcgag atcgcgcccg
     3481 agagatgcgg tccttttcca gggaggctgg tgaaattcgg cagcgcgtcg agcgaaagat
     3541 tgacctttgc ggaggagccg cgaatcttga actgattcac cttggtcaag aactcggtct
     3601 ccagatgacg ggcatcgatc aactttaaga aggttcgttt gggatcgacg ctggagacta
     3661 ttaccttagc tttgatctcc tcgcctgtgc cgagcgctac gccgaccgct cgactatttt
     3721 taacgagcac ttgatcgacc ggggactgag ttctgatgtc cgcgccatag tggcgcgccg
     3781 cctcggctat tacacggctg attgaacccg tgccgcctcg ggcaaaaccc caggatcgaa
     3841 acgcgccgtc gagctcaccc atataatgat ggagcagcac gtaggctgta cccggtgatc
     3901 tgggccccat gaaagtgccg atgatgccgc tggcggacat ggtggctttg aggacttcgg
     3961 tttcaaacca ctcgtcaaga aaatcggcgg agctcatggt cataagcttt gccagatcgt
     4021 agactagttt gtccccgagc cggcggaaat gattgcccag cgatatgagt ctataaagct
     4081 ctttaggatt ccatgaagtc ggctcgggag gaattaggcc aagcatcggc tttatcgcca
     4141 tagccatctg caccatgagc ttgccatact cttcgtaagc ttcggcatcg acggcagaat
     4201 gccgcgcgat ctcacgacgt gtcttggcgt gatcgcccca gcgagctaga tagtcgccgt
     4261 tgggcatcgg cgtaaatgtg ctatccagag gaatgatttc gagatcgtat ctgggcagct
     4321 cgagctcctg gatcacctcg ggtcgtagaa ggcttactac gtaggagcag acggagtatt
     4381 taaaacccgg atagagctcc tcggtaaccg cggcgccacc gagtatatga cggcgctcca
     4441 ggaccaggac ttttttccca gccttggcaa gatatgcagc cgcagcaaga ccgttatggc
     4501 cgccgccaat gataatcgca tcgtaagtcg tcatttattg atttgcttaa acgaacatat
     4561 ttggtaattt ctttacgaag tcaacgagcc ggcataattc acagtctccg aacagagaac
     4621 atacgcaagc aagatcgatt aaataacact gataaaatta agcgcgctcc tatcgagatt
     4681 atccactctc ccgcaattac catcgctaca ttttcatgcc cattggtact cctttccact
     4741 cacgcacgtc cgcgctctgc accagtcaga attggcgcac ctggtcagga tacttcgcgg
     4801 ccagctctta cgatgtggtg cacgactacg aatatcacgc gatccgaaat accgccggcc
     4861 tgatcgatat ctcaccgctt ttcaaatacg aggtgcgcgg caaagatgca ttgaagctgg
     4921 tcaatcgcgt gatcacgcga gacgtcgcca aatgcgcgat agggcaggcg gtttatggtt
     4981 gcttgtgtga cggcgaagga aaggtgattc aggatgggac ggtatttcgc ttgggtgagg
     5041 atcagtttcg ctttcatttg gccgagccgg ctctgcgttg gctgaaactc aatgccggag
     5101 gaatgtcggt ttcaattgac gaggtgtcgg agcaaatagc ggcccttgcg ttgcagggac
     5161 ccaacgcctt gaaaatccta acgcagatcg tcaatgccga tttgggcaag ctaaagttct
     5221 ttcgcttgat gccagccgat ttcgccggag tgccggttac cgtttcccgc accggttaca
     5281 ccggtgacct cggatacgaa atatggctgc ctgccgctga tgcagagcgt gtatgggacg
     5341 tgcttgccga taaaggcagt gtctatggac tgaaagcagc gggaatgctc gcgctcgatg
     5401 tcgcccggct cgaagcgggt ttcattcttc tcgaagtcga ttatatcggt gccgataagg
     5461 cactgatcga ttcgcagaaa tattcaccgt tcgagattgg tttggggtgg accgtggatc
     5521 tcacgaaaga ttttttcgtc ggcagcaagc agctccgtaa tgacgacgcc aaggttacgc
     5581 cgcgccaaat caccggcttg gaggtggatt ggcgccacta cgaagaactc tatcaaaagg
     5641 caggactccc gccgccggtt ccggcagccg cttggagagg cagtatcccg atttaccagg
     5701 acgatcttca ggtcgggcag gcgaccaccg gggcctggtc gcccagtgtt aaaaaataca
     5761 tcgtgttggc gacgatcaag agcgagcacg caaaccccgg aaccacactc cagatggaaa
     5821 cgacggtgga gcacgagcga aa
//
LOCUS       DASUVS010000293         5504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1650532, whole genome shotgun sequence.
ACCESSION   DASUVS010000293 DASUVS010000000
VERSION     DASUVS010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650532"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..1083
                     /locus_tag="VF182_15920"
     CDS             301..1083
                     /locus_tag="VF182_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438625.1"
                     /translation="MPGFGRRFVADPRDRNYLMRRLLPDARALTLPARKTWGINKTAL
                     NQGNTGTCVGHAWRNFLRCAPIQTIRPDPSPFDIYRSAVLKDPWTDNDDEANLPDGDS
                     GLDVGTSVRAGAEAVTGFGLLKSYVWAFELQPAVEWVLTEGPVVLGTNWYSSFQRPDN
                     EGIVRISADASVAGGHAYLWRGVDTRRALALCSNSWGDNWGRSGEFFLPFRDLERLIH
                     EDGEACSAVQQKLKAKAVMPLPRGAFDTESEDGGRKQRASRP"
     gene            complement(1165..1467)
                     /locus_tag="VF182_15925"
     CDS             complement(1165..1467)
                     /locus_tag="VF182_15925"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HEX6438626.1"
                     /translation="MREYGKNTTCIQVRVGERFMLALPAVATGGFTWLMDREPDVAVL
                     TQERIRPGGPGIGASSVQEFEFAGMHAGVSTLILEYKRPWENVKGERLEIKIVVEP"
     gene            complement(1666..2322)
                     /locus_tag="VF182_15930"
     CDS             complement(1666..2322)
                     /locus_tag="VF182_15930"
                     /inference="COORDINATES: protein motif:HMM:NF015336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438627.1"
                     /translation="MNNTKITAKAKNIAFAKDVTIQYAQADGTWNERPLSWQKNFTGY
                     DLFALTDNTFSTTEFVIRYTVNGQTFWDNNNGTNYHVDSARPNTVGGNVILNKATARR
                     GSQAGGGFVFTTSWVEGEIFIKNLSFQKHVGIRLSADGGASFHDTHASFSGNVPVAVG
                     VSQVEIWKLKTPELNLDQSTANFRFAIFYNNLNTGEWFWDNNFGQDYTLSKTDLASIE
                     "
     gene            complement(2497..3861)
                     /locus_tag="VF182_15935"
     CDS             complement(2497..3861)
                     /locus_tag="VF182_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438628.1"
                     /translation="MKAEKKNWQAPEFISREQCIKDTEEVLSVADIPTRQTEDIFRVN
                     VLGMDWDIGMVVHEPEDSSKIPIGADGKKAGFFLLHGGSSDFKGIERHAKLLAGKFGF
                     RVMAGTFPGRFYFPDPSRDWPDDTIHSDGTVRTPIWKQGELITPDQYDVVKDPSKRNR
                     YGTRTVARAKPDTNFWYRMAAWPAAFEEGMIEACRRHFPEKEFSIYGQGHSTGGPFIC
                     MLSQRIPNMAGVCATENSPFGYLCCERDKWGGDMGKITGYEKVEKKGQGRTDPFNELY
                     IRTWRDLARYQGPEALGQEGPAALMRLPAIMEDIFDAWEKAKNRPQFKAEYIVTHAIT
                     DSLAEGARVSAARLKMNKEETEALVQRFIGYTRELTGPNVKPVPPFLFEISKDSRDHS
                     PEVYNEVIIPGFRAMKPAPKIAIIRFGAGVHSFWKAEKDLPVGIVPSVIKSWKEAVMN
                     GYFA"
     gene            4342..>5504
                     /locus_tag="VF182_15940"
     CDS             4342..>5504
                     /locus_tag="VF182_15940"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005366383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEX6438629.1"
                     /translation="MKVLVLNCGSSTLKFQLIQTRGSAVERKLARGVVERIGGDSTYK
                     FEIIGEPPQQGNVAAENHEAATVQVIDWLNSHPNLGSVDAVGHRVVHGGDQFKSAVRI
                     DENVIKVLDSLCDLAPLHNPSSVSGIHAARKVLGSGVPMVAAFDTSFHQTMPQEAAIY
                     ALPYELSTKHHLRRYGFHGLAHQYSAMRYGELTGKALDQINIVTLHLGNGCSACAIRN
                     GVSIDTSMGYTPLEGLVMGTRSGDLDPALVVHLARHEGIDEVEKLLNTGSGLLGISGI
                     SSDMRELVAVYQENPRARLAIDVFCYRARKYLGAYLAVLQGADAVIFSGGIGEHLPLI
                     RKEICGGMDWCGLILDESQNESATGTEGRISAAEARIQVFVIPSDEEAVIARQT"
BASE COUNT         1155 a         1515 c         1532 g         1302 t
ORIGIN      
        1 gatctctgcc catggttcac ggcgcaagga acctggatca gcaatgtttt caaggtccca
       61 aacgacaagg agctgttcat ctgtaaaatt gcatgatggc actatatctg ctctaacaag
      121 cgccaagacg tcaaggcgcg acgtggacag agtaacggca cagccgcaac aaggttttgt
      181 aaaaatagtt tgtcagaaag aagatttcgc tgtgacttga gtcgaaaagg taaccttccg
      241 gaatctttac atgatagagt gaccgcctta attcttatct agacaacacg gaggtgtttt
      301 atgccgggat ttggcagacg attcgtagcg gatcctcgag atcgcaacta cctcatgcgc
      361 cgcttattac ccgatgcgcg agctctcact ttgcctgcgc gtaagacgtg gggcatcaat
      421 aaaactgctc tcaatcaggg caacaccggg acgtgcgtgg gacacgcgtg gcgtaacttt
      481 cttcgctgtg cgccaatcca gacgataaga cctgatccat cgccgttcga tatctaccgc
      541 tcggccgtct tgaaggatcc gtggacggac aatgatgacg aggccaatct tccggacggt
      601 gacagcgggc tcgatgtcgg gacctcagtg cgcgccggcg ccgaggccgt cacaggtttt
      661 ggcttgctca aatcctatgt atgggcattc gagctgcagc cggcagttga atgggtgctc
      721 accgagggac cggtcgtgct cggcactaat tggtactcga gctttcagcg gcccgacaac
      781 gagggtatcg tccggatctc agcggacgcc tcagtggccg gcggccatgc gtacctctgg
      841 cgcggtgtcg atacccgaag agcgcttgcg ctgtgctcca actcgtgggg tgataactgg
      901 ggcaggtccg gcgagttttt cctgcccttc cgggatctcg aacgcttaat ccatgaagac
      961 ggcgaggcct gctccgcggt tcagcagaaa ctcaaagcca aagcggttat gccgctgccg
     1021 agaggagcgt tcgacaccga gtccgaagac ggcggccgca aacagcgtgc gtctcggccg
     1081 tgacggctgg gcgcaagcca agccctcgct aacacgaaga tttgggggca gccggtagct
     1141 accggctgcc cgcgctcata cggtttacgg ctccactacg atttttatct cgagccgctc
     1201 tcccttgacg ttttcccacg gccgcttgta ttccaaaatc aaggtgctca ctccagcgtg
     1261 catgccagca aactcgaatt cctgaacgga ggatgcgcca atcccgggac cacccggccg
     1321 gatccgctcc tgcgtgagta cagccacgtc gggctctcga tccatcagcc atgtgaagcc
     1381 gccggttgcc actgcgggaa gcgccagcat gaagcgttct ccaacacgga cctgtatgca
     1441 tgtcgtgttc tttccgtact cgcgcatgtg tcctcaccac cgaagccgaa aaagaagacg
     1501 cggcaccggc ttcacgttgg acccgtaagc ttccacccat aacccaggac cactgcgcca
     1561 attgcggttt ctttcttcct ttccatcttc aatcatgccg cgctaactcc tggccccttc
     1621 ggccgttaaa ttgggcaatc gatagttgct cctaggtctg tcggcttatt cgatactggc
     1681 gaggtcggtt ttactcagcg tgtagtcctg gccgaagttg ttgtcccaga accactcgcc
     1741 ggtatttaga ttattgtaga agatcgcgaa tctgaaattg gcagtgcttt gatcgagatt
     1801 taattccggt gtcttgagct tccagatctc cacctgcgac actcctacgg cgacaggaac
     1861 gtttccgctg aacgatgcat gggtatcgtg aaagctcgcg cctccgtctg ctgagagccg
     1921 gatgcccacg tgtttttgga aggagaggtt cttgatgaag atttcccctt ctacccagga
     1981 agtggtgaag acgaacccac cgcccgcttg agaacctcgc cgcgccgtcg ccttgttcaa
     2041 aatcacgttc cctccgactg tattcggccg tgccgagtcg acgtgatagt tcgttccgtt
     2101 gttgttatcc cagaatgtct gaccattgac agtgtagcgg atgacgaatt cggtggtgga
     2161 gaaggtgttg tcggttagcg cgaatagatc gtaaccggtg aaatttttct gccatgataa
     2221 cggtcgttca ttccatgtcc catcggcttg ggcgtactga atcgtcacat ccttggcaaa
     2281 ggcgatgttc ttcgctttcg ccgtgatctt ggtgttattc acgccggtta ctccgccgcc
     2341 gggggaaaag ttgtccgact cgatgtattt caatctgatg ggttggtctg cgaccaatga
     2401 ttcctaccgt acgctgtgag cgttcatctt aatgcaaaga tggaagtttc agcatgtcgt
     2461 ctagtcggtg aatagattcg agaactcacg aacccgctaa gcgaagtacc cgttcatcac
     2521 tgcttccttc catgacttga tcaccgacgg cacaatcccc accggcaaat ccttttccgc
     2581 tttccaaaaa ctatgcacgc cggcgccgaa acggatgatg gcgatcttcg gcgcgggttt
     2641 catggcgcga aagccgggaa tgatcacctc gttgtaaacc tcggggctat gatcgcggct
     2701 gtccttggat atttcgaaga gaaaaggcgg caccggtttg acgttcgggc cggtaagctc
     2761 gcgcgtatag ccgatgaagc gctgcaccag cgcttcggtt tcttccttgt tcatcttcaa
     2821 tcgcgcggcg ctgacccgtg ctccttctgc cagtgaatcc gtgatcgcgt gcgtgacgat
     2881 atattcggct ttgaattgcg gacggttctt ggctttctcc catgcatcga agatgtcttc
     2941 catgatcgcg ggtaagcgca tgagagccgc cggcccttct tgtcctagtg cttccggtcc
     3001 ctggtaacgc gcgaggtcgc gccaagtgcg tatgtaaagc tcgttgaacg ggtcggtccg
     3061 gccctggccc ttcttttcga ctttctcgta gccggtgatc tttcccatgt cgccgcccca
     3121 tttgtcgcgc tcgcagcaga gatagccgaa gggcgaattt tccgtggcgc agacgcccgc
     3181 catgttggga atacgctgcg acagcatgca gatgaacggc ccgccggtgg agtgaccctg
     3241 gccgtaaata gaaaattctt tttccgggaa gtggcgccgg caagcttcga tcatcccttc
     3301 ttcaaaagcc gccggccagg cggccatgcg ataccagaaa ttagtatccg gcttagcccg
     3361 cgcaacggtg cgggtgccgt agcgattgcg cttggatgga tctttgacga catcgtattg
     3421 atcgggtgtg atcaactcgc cttgcttcca gatgggcgtg cgcacggtgc catcggagtg
     3481 aatcgtatcg tcgggccaat cccggctcgg atcggggaaa tagaatctgc caggaaaagt
     3541 gccggccatc actctgaagc cgaacttgcc ggcgagcagc ttggcatgac gctcaatgcc
     3601 tttgaaatcg ctcgagccgc catgaagtaa gaagaagccg gcctttttgc cgtcggcgcc
     3661 aatcgggatc ttgcttgagt cctccggttc atggacgacc atgccgatat cccaatccat
     3721 gcccaggaca ttgacccgaa aaatatcctc ggtctgcctg gtcggaatgt cggccacgct
     3781 gagaacttcc tctgtgtctt tgatgcactg ttctcgtgag atgaattcag gcgcctgcca
     3841 gtttttcttt tcggctttca tatctggttg ctccccatat ttcgattaaa gtggtttgac
     3901 gttactcctt cagaccctat atgatccgca actttagtgt caacattgga tgaccttaga
     3961 aaagcataga acagaaatcc gcccatcgtg acgcagttgc tgttcgccgt gaacaacagc
     4021 cactccggta aaagcgccgg agcttgctgc gtgagacttg ttctaagatc cctcaaagtt
     4081 cgtcattccc gaatgcttgt atcgggaatc taggcgagat tggagctgga ccacgattaa
     4141 taaattcccg ggtacgcctg aggtaattct gactgctatc ttcgatcctc cggagccggt
     4201 gtggcgccgt tcggcagcga gaaagattct cacgcgctca gactgacaac taaataggca
     4261 attgatatct cgttgaggtg agatacctga gctaaatcaa ctggcagtct ctacgtcaaa
     4321 agattattgt acagtaacat tatgaaagtt ctggtgctta actgcggcag ctcgactctc
     4381 aagttccagc tgattcagac tcgcggcagc gctgtcgagc gcaagctggc acgcggcgtg
     4441 gtcgagcgga tcggcgggga ttcgacttat aaattcgaaa tcatcggcga gcctccgcag
     4501 caggggaacg ttgctgcgga gaatcatgaa gcggcaacgg tacaagtgat cgattggtta
     4561 aactcgcatc ccaatttggg ttctgtggac gccgtcggtc atcgggtcgt tcatggcggc
     4621 gatcaattca aatccgccgt gcgcatcgat gagaatgtca tcaaagtcct cgattcgctg
     4681 tgcgatctgg cgccgcttca caatccgagc agcgtcagcg gtatccatgc cgcgcgcaag
     4741 gtcttaggat cgggcgtgcc gatggtggcg gcgttcgata cttcttttca ccagacgatg
     4801 ccgcaagagg cggcgattta cgctctgccg tacgagctat cgaccaagca tcatctgcgg
     4861 cgttatgggt tccatggtct ggcgcatcag tattcggcca tgcgttatgg cgagcttacg
     4921 ggaaaagcgc tcgatcagat caacatcgtc accttgcatt tggggaacgg ttgctcggct
     4981 tgcgcgattc gcaatggcgt atcgatcgat acgtcgatgg gctacacgcc gctcgaagga
     5041 ctggtgatgg gaactcgctc cggcgatttg gatccggcat tggtcgtcca tctcgcccgg
     5101 cacgagggca tcgatgaggt cgagaaactg ttgaataccg gctcgggatt gctagggatc
     5161 tcgggaatct ccagcgacat gcgcgagctg gttgccgttt atcaagagaa tccgcgcgcc
     5221 cgcctggcca tagacgtgtt ctgttaccga gcgcgcaaat atctcggcgc ctacttggcg
     5281 gtactgcaag gagccgatgc cgtgatcttt agcggcggca tcggcgaaca cctgccgctg
     5341 atccgcaaag aaatctgcgg cggcatggac tggtgcggtt tgatcctcga tgaaagtcaa
     5401 aatgaatcag ctactggaac agaagggcgc atttccgcag cagaagcgcg cattcaagtg
     5461 tttgtgattc ccagtgatga ggaagccgtc atcgcccgcc agac
//
LOCUS       DASUVS010000294         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_339053, whole genome shotgun sequence.
ACCESSION   DASUVS010000294 DASUVS010000000
VERSION     DASUVS010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339053"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VF182_15945"
     CDS             <1..606
                     /locus_tag="VF182_15945"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6438630.1"
                     /translation="ATLDVILGAVIAFLLLRSRVRGRNVLDAIATMPLAIPGVVLAVG
                     YLRVFHGWDFPGIGGPLTSSWLILVIAYTMRRLPYTVRASYAALQQVHVTLEESAQSL
                     GANRFGTFRKITLPMITGGLLAGGLIAFVTSSVELASTIMLVPRIELGPISYGIYLYM
                     QSPLGRGAGAALGVVAILLVAIGTYLTHRIFGGRAGNAFRI"
     gene            608..1702
                     /locus_tag="VF182_15950"
     CDS             608..1702
                     /locus_tag="VF182_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6438631.1"
                     /translation="MQPASVRLESVGKRYGDHWVVCSVSLNIKRGEFFTFLGPSGCGK
                     TTLLRIVAGFVAPDRGVVYLDDQPMNHVPPWRREIGLVFQNYALWPHLSVFENIAFGL
                     RERKVARGEIERRVAAALKQVDLAGADKRRPSQLSGGQQQRVALARTLVIQPRVLLLD
                     EPLSNLDAKLRIEMRLELLKLQRDLGLTTSYVTHDQEEALAMSTRIAVMRDGNVIQQG
                     KPREIYEQPADQFVAAFLGHANLFSGHVVSSGEGSIDVDVGGGIAIRASCVTPSSPTR
                     GGERLFLSVRPEAVELQEIGTVCNGVNHIEGQVIAAAYRGSLIEYEIGAGDHIIKAHV
                     VNPKGKPLFERNQPVCLRFAPDDVTLVNCD"
     gene            1838..>2088
                     /locus_tag="VF182_15955"
     CDS             1838..>2088
                     /locus_tag="VF182_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438632.1"
                     /translation="MKKRKYLLIVPFTLFFLAGISRLIYTQRFAQEAAILKINQEYEE
                     KTIIKQSRIPGAGNGLYAAVKINKGETIGELGGRLVSDD"
BASE COUNT          457 a          563 c          591 g          477 t
ORIGIN      
        1 gcaacgctgg atgtcattct tggcgccgtg atcgcgttcc tgctgttgcg cagtcgggtg
       61 cgcgggagaa atgtccttga cgccatcgcg acgatgccgc tggcgattcc cggtgtggtg
      121 ctcgctgtcg gttatctgcg ggtctttcat ggttgggact tccccggcat cggcgggccg
      181 ttgacctcat cctggctgat actggtgatc gcttacacga tgcgccgcct gccctatacc
      241 gtgcgcgcca gctacgcggc tctccaacaa gttcacgtta ctctcgaaga atcggcgcaa
      301 agcctcggag cgaaccggtt tggcaccttt aggaaaatca ctctgccgat gatcaccggg
      361 ggtttgctgg ccggcggatt gatcgctttc gtcacttcat ccgtggagct cgcatcgact
      421 atcatgctgg tgccgcggat tgaattggga ccgatttcct atggtattta tctctacatg
      481 caaagcccgc tgggccgcgg cgccggcgcc gcgctcggag tcgtcgcgat tctactggtg
      541 gccatcggaa cttatctgac gcaccgaatc ttcggcggcc gcgccggtaa cgcttttcgc
      601 atttgatatg caaccggcga gtgtcaggct cgagagtgtc ggcaaacgtt acggcgacca
      661 ttgggtggtt tgcagtgtca gcctcaatat caagcgggga gaattcttca cttttctcgg
      721 accgagcggc tgcggcaaaa ccaccttgtt gaggatcgtt gcgggatttg ttgcacccga
      781 tcggggcgtc gtctatttgg acgaccaacc gatgaaccac gtgccgccct ggcggcggga
      841 gatcggcttg gtttttcaaa attacgcctt gtggccgcac ctgagcgttt tcgagaacat
      901 cgcctttggt ttgcgagaac gaaaagttgc gcgtggcgag atcgagcgcc gagttgcggc
      961 ggctctcaag caagtcgatc ttgcaggagc tgacaaacga cgtccatcac agctttccgg
     1021 cggccagcag caacgagtcg ctctggcacg gaccttagta atccagccgc gagtgctgct
     1081 tctcgacgag cctctcagca atcttgacgc caaacttcgc atcgaaatgc gtctggagct
     1141 gctcaagctg caacgggacc tgggcctgac gacgagttat gtgactcatg atcaggagga
     1201 agctttagcc atgtcgacgc gcatcgccgt tatgagggac ggcaacgtca tacaacaagg
     1261 gaagccgcgc gaaatttacg aacaaccagc cgaccaattt gttgcggcat ttttggggca
     1321 tgccaatcta ttttcaggtc acgtcgtatc ctctggcgag gggtcgatcg acgttgatgt
     1381 tggcggcggc atcgctatac gcgccagctg cgtaaccccc tcgagtccta ctagaggtgg
     1441 agaaaggctc tttctcagtg ttcgtcccga ggccgtcgag ctccaggaga tcggcacggt
     1501 ttgcaacggc gtcaatcaca tcgaaggaca ggtgattgcg gctgcgtatc gaggatcgct
     1561 tatcgaatat gagattggtg ccggcgatca catcatcaaa gctcacgtgg tcaatcccaa
     1621 ggggaaaccc ttgtttgaac gaaaccagcc cgtctgcctc cgcttcgctc ccgacgatgt
     1681 aactttagtg aattgtgatt gacagcatca aggatcgttg cctaactacc tgtatcgcgc
     1741 tcaagtggag aaggcgagaa aagcggttac gcatttctaa tgcctgaccc gatgccaccg
     1801 accagggagg aagttccaaa ttttaatcag agaccctatg aaaaaacgaa aatacctttt
     1861 gatcgtgcca ttcacactat tctttctcgc aggcatatcg aggttgattt acactcaaag
     1921 attcgcccaa gaagcggcaa tcttaaaaat caatcaagag tacgaagaaa agaccataat
     1981 aaaacaatcg agaattcccg gtgcgggcaa cggattgtat gcggcagtaa agatcaacaa
     2041 gggcgaaacc atcggcgaat tgggcggccg tctggtctct gatgacga
//
LOCUS       DASUVS010000295        14861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1213691, whole genome shotgun sequence.
ACCESSION   DASUVS010000295 DASUVS010000000
VERSION     DASUVS010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14861
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213691"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..1333
                     /locus_tag="VF182_15960"
     CDS             350..1333
                     /locus_tag="VF182_15960"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6438633.1"
                     /translation="MGLMVHGEWHDDDAIPSDAAGHFIRAASRFRHWITADGAPGTSG
                     EGGFIAEPGRYHLFVSYNCPWAHRAIIMRKLKKLEDVISMSLADKPKTEGWSYSLGID
                     DLEPSADGIFRLHQIYKAADSDYSGKVTVPTLWDRERRTIVNNESSEIIRMLNSAFAK
                     WTDVAYDFYPAKSRHDIDSVNDFVYDHLNNGVYRAGFARTQKAYDEAVRKVFRGLDSL
                     EERLSNTRYLAGGRITEADWRAFPTLLRFDLVYYGHFKCNLRRIQDYPRLVEYLRELY
                     QWPGIKETFDLEKIKAGYYSQRNINPTGIVPIGPDLTHLEQPHQRGPDVAI"
     gene            1500..2027
                     /locus_tag="VF182_15965"
     CDS             1500..2027
                     /locus_tag="VF182_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438634.1"
                     /translation="MKNVRSFIVGSAILTVATGAVMSPPSTTFAQNEPAGKGGFGQRS
                     RMHRMADSGAPLISIALRHRTELNLSNEQVASLEKIRTHYQEQGAPHQQQLRALESEI
                     SELRQQSPANLIQMKLKIEQSEKLRSDLRYLREEALENGKTILTAAQRDQLKTLAGSG
                     HRGFRKGQSETPKQS"
     gene            complement(2100..2666)
                     /locus_tag="VF182_15970"
     CDS             complement(2100..2666)
                     /locus_tag="VF182_15970"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6438635.1"
                     /translation="MKYLVNRTFLTALILAFCIPAWAQDIPFVPSPMIVVDRMLELAE
                     VKRDDIIYDLGSGDGRILIQAAKKYGARGVGIDMNPKLVADATRKAAEEKVDHLVQFR
                     AADGLTVDISEATVVTLYMFKWFNNQMRPKLQGLKAGARVVAHDFDIDEWQPNKVESV
                     EPKSDPSGELTHTRTLYLWKVAAPAQVP"
     gene            complement(2692..3792)
                     /locus_tag="VF182_15975"
     CDS             complement(2692..3792)
                     /locus_tag="VF182_15975"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438636.1"
                     /translation="MTSAATDPIIDADAHVVESDHTWDYLDPSDRQYRPQPLESREDA
                     GVKLQFWLIDGKVRGFRFPAFSAEELERRSRQVGRKFADIKESREMGNVDLRLDYMDQ
                     TGVDIQVLHNTMFIESATERAPVEVALCKGWNRWLADIWRQGKGRLRWSCMAPTLSMC
                     DALDQIRFAKENGACAVLMRPVEGIRLLADPYFYPVYDEAQKLDMAIAVHIANGSPWL
                     NDLYRHPVPVAATLHRFRIPTVAAFNDVLLSEIPHQFPKLRWGFVEASSQWLPWVLHE
                     AKNRFKTLGREWPKNFAREYRMFVTCENSDDLPYIINEAGEDCLVVGTDYGHTDISSD
                     IDAIKLFRQRTDLPDGVKRKILSDNARALYAI"
     gene            complement(3946..4767)
                     /locus_tag="VF182_15980"
     CDS             complement(3946..4767)
                     /locus_tag="VF182_15980"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6438637.1"
                     /translation="MNLSRRMRPLMLALCVSVFGACAGAQSQPNRQGYEPRVGQQGKD
                     VVWVPTPQQLVDKMLDMAKVTPKDYLIDLGSGDGRTVITAAKRGLRAHGIEYNPDMVE
                     LSKSNAAKAGVSDKATFAKADLFESDFSDAQVITMFLLPQINLKLRPKILDLKPGTRI
                     VSNSFTMGEWEADESATVGGECDHWCTALLWIVPAKVEGSWQLPQGELSLKQNFQMLS
                     GTIKTGNSIAEITQGKMIGDQILFTAGGNHYTGRVNGNVMEGKTGSGTWKGMRVK"
     gene            complement(4814..5638)
                     /locus_tag="VF182_15985"
     CDS             complement(4814..5638)
                     /locus_tag="VF182_15985"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6438638.1"
                     /translation="MNASHICRRLFLALFIAALSAGPAAQAQPAKQEFKPEVGQEGKD
                     VIWVPTPQELVEKMLDMAKVTSKDFVIDLGSGDGRTVIAAAKRGAKALGIEYNPDMVE
                     LSKRNASKESVSDRASFMKADLFETDLSQAQVITMFLLPDINLKLRPKLLNLRPGTRI
                     VSNSFTMGEWQADETANVKNGCASYCTAYLWIVPAKVDGTWQLPQGELVLKQTFQMVS
                     GVLKTGNNTGPINGKVTGDQIRFTAGGTEYQGRISGSSIEGSSKTGGTFKATRAGK"
     gene            5846..7000
                     /locus_tag="VF182_15990"
     CDS             5846..7000
                     /locus_tag="VF182_15990"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX6438639.1"
                     /translation="MRQRSDYPIFSDREIATRHQAVYRLMEQENVDALLIYGAGRYAS
                     DVYWLSDWPSSREAYVLFQTGKEPVILLQLFNHFPMAKVMSVIKDVRWAGANTTSSVL
                     DLIRERGLDSKKIGIVGSLPYQVYLRIAEKFPGAALSDLSGKLRMMRTIRSAEEIERI
                     RFASKLTDESIEALAGGLKAGMREDEIAALIEPAYLKRGGYAGIHFMSSMPMRDPDFP
                     VPAQFQSSRKLRKGDCLITEISGAYSGYSGQIHRTFSVGEGPTPEWQQMHAAAVEAFE
                     ILSEVIKDGATTTEAEEAAEIIHRRGYSTYDDLVHGVNQYPPIFQTKTRKRHDSKEMV
                     FRENMVIVIQPNLITEDEKMGLQFGETLLVTKKGCETLNAYPREWVICQG"
     gene            complement(7240..8118)
                     /locus_tag="VF182_15995"
     CDS             complement(7240..8118)
                     /locus_tag="VF182_15995"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6438640.1"
                     /translation="MWFDVPAQIIALAAALSYAASGIAAKRGLRYSTPITVTLVSVTI
                     HAVTLSAAVLLTGGIPTVSWWVLFLFAVTGTLQPIIRLFTYAGIHYIGASRGTTVRGA
                     HPLFSTALAIIFLGEQATLPVIGGTVLIVGGITLISWQTEAQRAGFRWWYIGYPLGAA
                     FLAGISHPLRRYALSLANEPLYLAAVIGVVALPWLASFVVLPRKGAEQPVWNRESIGA
                     FLFAGVFETLGIVLVIAALSVGAVVIVSPIVATSPLWILLGTWLFLRGIERLTVRTII
                     GALCVVAGTIAISLVR"
     gene            complement(8148..9176)
                     /locus_tag="VF182_16000"
     CDS             complement(8148..9176)
                     /locus_tag="VF182_16000"
                     /EC_number="1.1.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005962 - mitochondrial isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_component: GO:0045243 - cytosolic isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_function: GO:0004449 - isocitrate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NAD(+))"
                     /protein_id="HEX6438641.1"
                     /translation="MMWKDLRVKRHKITLIPGDGIGPEVVKPTLKLIEATGVKIEWDS
                     HLAGAGALKKHGTTLPKQMIDSFAKNRVALKGPVTTPVGEGFASVNVELRQSFDLYAN
                     LRPVKNLPGVKARYENVDLIVVRENTEGQYSGIEHEVVPGVMESLKIITEKASTRIAK
                     FAFDYAVANGRKKIAAVHKANIMKLTDGLFLECARNMAQKYKKIAFSDLIVDNACLQL
                     VLDPTRLDVLLLENLYGDIVSDLAAGLVGGLGVVPGANLGDNHALFESVHGSAPDIKG
                     KNIANPTAIILAAVMMLDYISEAKAAARLRAALHAVLMRGECLTQDLGGTASTSQFAD
                     AIRREIEK"
     gene            9414..10688
                     /locus_tag="VF182_16005"
     CDS             9414..10688
                     /locus_tag="VF182_16005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6438642.1"
                     /translation="MLRKLRDRLAGLFFGWRMVGLSCAIRVLGGGLHAYGFTVFFLPI
                     TQELGLSRAATSLVFSLARAQGAIEGPVAGYCIDRYGPRPVIFVATLLAGIGYMLLAA
                     VHSYTALLIVYMGIISLSYQAGFMDATMMVANSWFIRKRALAMSLTSGSIALGGTLVT
                     PFLAYAVHAWGWRRAAIGAGAAFLIGGLPLSLLLRRSPESMGLLPDGEPLPKALDATG
                     STHAVEEANFSLQQTLRLRPFWLLSFATTFRVVCSSCILVHFVPILVWRGLTEQRAAF
                     FLAFIALLGMPAHLLIGWLGDRLDKPKLMSVCMMIASLSVYLLFSYAAQWQLWLALVL
                     FTVIEGLFPVTWATVGDFFGRNNFAKVRGSMSFLYMWGSVIGPVIAGAVYDRTQSYRP
                     LMWGLISLCWITALLYAVLAKPIALSSEPVNL"
     gene            10685..11134
                     /locus_tag="VF182_16010"
     CDS             10685..11134
                     /locus_tag="VF182_16010"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX6438643.1"
                     /translation="MNRLSKHTGKTFINRWLPVLLWATVIFLFSTEHFAAPQSSRILG
                     PLLRSAFPGITAEQVSLVQFVVRKLAHWSEYFILAVLLYRAWYAENSGRSPVRPAALT
                     VCFALVWAISDEFHQSLVPSRTASLVDVMIDGFGALCGSFWMYLRHR"
     gene            11378..11815
                     /gene="pal"
                     /locus_tag="VF182_16015"
     CDS             11378..11815
                     /gene="pal"
                     /locus_tag="VF182_16015"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX6438644.1"
                     /translation="MPAELLKGAAYSPSAMEALSRGLAVITPPGAALQDVYYEFDSID
                     LKADAEAILKKNAEWMKANPLARVEVEGHCDDVGSAEYNLALGAKRAQTARDFLVQQG
                     IAPDRLVTISYGKEAPACLEQNEDCRVKNRRARFVMFTELPTS"
     gene            complement(11965..14547)
                     /locus_tag="VF182_16020"
     CDS             complement(11965..14547)
                     /locus_tag="VF182_16020"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX6438645.1"
                     /translation="MLRLLETVSWRHLKLHRLRTLLTILGIALGVAVIVAIAIVNRSL
                     TTSFQSTIDQIAGKAVLQVANGESGIREDLFRVIRDTPGVQDAAAAVDGFLPVSGAAA
                     ERLYVYGVDLLTDFAIRDHRFAGAGFEFDRALDFIAAPDSIAVTETFAGRLALNLGSE
                     LTVITSRGKQTYTVRALLKEEGTAKVFGGNFALMDLPVAQRAFGKQGKLDIVDLTVEP
                     GANVADVQQRLRQSLQGMADVERPRKRGEQIESLLTSFRVGLFFVSLIALFVGFFLIY
                     NTVSVSVVQRKREIGTLRCIGMRRGELIRWIILEALLLALVGALAGCVFGWLLAQAAL
                     VAVGETVSNLFSSIGLMTAGLTWQELLLALASGVGVAVLAALHPAVEAVRISPLENAR
                     QSVWRPVFHGSKSWGTRLGLLCFLLSPMIIFFAALLRSPVQQFSAGVIGMLIFLLALA
                     FLCPLIISSCVNWFGRAGITIPGFSWAESRLASDSLRKNPIRSGITVATMVISLAAIF
                     TIAAFVNSVRGSLLAWIDQMVTADLIVSSGARTAGPKNVPLTEAPLQGLRSVSGVKIV
                     DLYRLIRSNYQGRPILIESFSAAASAAVRKLPTPSGEGKKVLSEMAAGKGVIVSESFQ
                     SKFGKGANDTVELATPAGLVAFRVLGVYIDYSSDIGSVLIDRALYKKFWQDELVDAFD
                     LWLEPGADQNIVIEQIKKDYGEQYQLFISTHRQLKESVVRIMEQSFVVNYAVEIVAVI
                     VAVFSVINTLLASVLDRARQIGVLRAIGATQAQIRRIIIFEAGWMGLIGGFLGLIAGT
                     IMSYHHVVYNTKVLTGWTFEYYYPYDLAALAIFVSVILCLLAGYWPAKQAAATPIVSA
                     IGYE"
     gene            complement(14547..>14861)
                     /locus_tag="VF182_16025"
     CDS             complement(14547..>14861)
                     /locus_tag="VF182_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6438646.1"
                     /translation="QLAKFLLDKVGLSPRGSHLPEAMSGGEIQRLAIARALAFEPPII
                     LADEPTGNLDSKNGEIVLNLLRRINQDDRCTVVMVTHSPEAAKVGDRTICLRDGLIEK
                     DF"
BASE COUNT         3382 a         4229 c         3983 g         3267 t
ORIGIN      
        1 ctcacggagg aaggacaaaa agccatggct ctcggtgggc gcatacccgc tcatccgaaa
       61 gtcgaaccca ccgataaagt gcgacctgaa acgatctatc ccttgactgt tgctgactac
      121 aaggagtttc cgaagtacaa caagctctgg aaagaaatct tcaagcttcg ttaaattggc
      181 aaacacaacc tgtaaagagc ggagatcagt ggccaagatc agcgatcagc atccatgtgc
      241 atcttccaac atgagcaagg caggttgagc tttccggata aagctgattt ccgttccgct
      301 gctcctgctt agtcgaactt taatcgagcc gtttacggag gtgcttgtta tgggcttaat
      361 ggtgcatggt gaatggcacg acgacgatgc aatcccgagc gatgcggcgg gccattttat
      421 ccgcgcggct tctcggttcc gccactggat cacagccgat ggcgctcccg gaacttccgg
      481 cgagggtggt ttcatagccg aaccgggccg ctaccatctg tttgtctctt acaattgccc
      541 ctgggcgcat cgcgcgatta tcatgcgcaa gctcaagaag ctcgaagatg ttatatcgat
      601 gtctctcgcc gacaagccaa aaaccgaagg ctggtcctat tccctcggaa tcgacgatct
      661 agaaccctct gccgatggca tctttcgcct gcaccagatc tataaagcgg cggatagcga
      721 ttacagcggc aaggtaaccg tgccaacttt gtgggaccgg gagcggcgca cgatcgtcaa
      781 taacgaatca tcggaaatca ttcgcatgct gaattccgcc ttcgccaagt ggaccgacgt
      841 ggcctacgat ttttatcccg caaagtcgcg tcacgacatc gattccgtca acgatttcgt
      901 ttacgaccat ctaaacaacg gggtttatcg cgccggcttt gcccggaccc agaaggcgta
      961 tgacgaagcg gtgcgaaagg tttttcgcgg gctcgacagc ttggaggaaa gattaagcaa
     1021 cactcgttat cttgccggcg gacgaatcac cgaggcggac tggcgagcat ttcccaccct
     1081 actgcgcttc gacctggttt actacggtca tttcaaatgc aatttgcgcc ggatccagga
     1141 ctatccacgt ctagtggaat atttacgcga gctttatcaa tggcccggaa tcaaagagac
     1201 tttcgacctc gagaagatca aggccggata ctacagccag cgaaacatta atcctaccgg
     1261 catcgtgccg atcggacccg atctaactca cctcgaacaa ccgcaccagc gcggacctga
     1321 cgttgcgatt taaccattcg aatgtcgctc gctttttcct gctccgatgt cgagcgagga
     1381 gaatgcgcat ctcggtcacc ggccacgcct aaaacctgcc actaacgcca tccatgtaaa
     1441 ccttccgtcc cggagcgcgt ctaatcattc aaattctttc acctttagga ggggacttta
     1501 tgaaaaatgt ccgttcgttt attgttggca gtgccatctt gacggtggct accggcgcag
     1561 tcatgtcgcc gccatcgacc acattcgccc agaatgaacc agcaggcaaa ggcggatttg
     1621 ggcagcgctc gcgaatgcat cgcatggccg atagcggggc gccgttgatt agcatcgcac
     1681 tcaggcatcg caccgagttg aatctatcga atgagcaagt ggcaagcctg gagaagatca
     1741 gaacccatta ccaggaacag ggtgctccgc accagcagca gttgcgggcc ctcgagagcg
     1801 agatctcgga attgcgccag caaagcccgg caaacttgat ccagatgaag ttaaagattg
     1861 aacagtccga gaagctgcgc tccgacttgc gttacctgcg cgaagaagcg ctggagaacg
     1921 gtaaaacgat tctaactgca gcgcagagag atcagcttaa aaccctagcg ggctcgggtc
     1981 atcgcggatt tcgaaaagga cagagcgaga cacctaaaca aagttagagg tcgatctgac
     2041 taccgcaagg cccgagggca ttcctcgggt cttgccatta ttccggatag ttcaggtcct
     2101 catggtacct gagccggcgc tgcaactttc cagagataaa gtgtgcgggt atgggtcaac
     2161 tcgcctgaag gatctgactt cggttcgaca ctttcaactt tgttaggttg ccactcatcg
     2221 atgtcgaaat cgtgcgcgac caccctcgcg cccgccttta gcccctgcaa tttcggccgc
     2281 atctgattgt taaaccactt gaacatatat agcgtgacca ccgtcgcttc cgagatatca
     2341 acggtgagtc catccgccgc gcggaactgc actaggtggt cgaccttttc ctcggcggct
     2401 ttgcgtgtcg catccgccac cagcttcggg ttcatatcga ttccgacccc gcgagccccg
     2461 tacttcttgg ccgcttgaat cagtatgcgg ccatcgccgc tgcccaaatc gtaaatgatg
     2521 tcgtctctct tcacctcggc gagctcgagc attcggtcga ccacgatcat cggcgatgga
     2581 acgaacggaa tatcctgcgc ccaagcggga atacagaaag ccagaatcaa ggcagttaga
     2641 aaagttctgt tcaccaagta cttcatcgta agcgtctctt tcctcaaaat tttagattgc
     2701 atagagcgca cgggcattgt cactgagtat tttacgcttc actccgtccg gcaaatcggt
     2761 ccgttgccta aacagcttga ttgcgtcgat gtcgctcgat atatccgtgt ggccataatc
     2821 ggttccgacc accaaacagt cctcgccggc ctcatttatg atatacggca agtcgtccga
     2881 attctcgcag gtcacgaaca tgcgatactc gcgcgcgaaa tttttcggcc attcgcgccc
     2941 taaggttttg aagcgattct tcgcttcgtg taacacccaa ggcagccact gcgagctggc
     3001 ttcgacaaag ccccatctca gtttagggaa ctggtgcggt atttcgctga gcagcacatc
     3061 gttgaaagcc gccaccgtgg ggattctaaa tcgatgtagg gtggcagcaa ccggcactgg
     3121 atggcgatag agatcattga gccaggggct accattggcg atgtgaacgg caatggccat
     3181 gtcgagcttt tgcgcctcgt catagaccgg atagaaatac ggatccgcca gcaaacgaat
     3241 gccttcaacc gggcgcatca gtaccgcgca cgcgccgttc tccttggcaa agcgaatctg
     3301 gtccagcgca tcgcacatgc tcagggtcgg cgccatacag gaccaacgca ggcggccctt
     3361 gccctggcgc caaatgtcag ccaaccagcg gttccaaccc ttgcatagcg cgacctctac
     3421 cggtgcgcgc tcggttgcgg actcaatgaa catggtgttg tgcagcacct gaatatccac
     3481 tccggtctgg tccatataat ccaggcgcag gtcgacgttg cccatttcac gagattcttt
     3541 gatatcggca aatttgcgac cgacctgacg agaacgccgt tccagttctt cggcagaaaa
     3601 agccgggaag cgaaaccctc ttactttgcc gtcgatgagc cagaattgca gcttgacccc
     3661 ggcatcttcg cggctctcca aaggttgcgg tcgatactgc cggtccgacg gatcgagata
     3721 atcccaagta tgatccgact cgacgacgtg agcatcagca tcgatgatcg gatcagtcgc
     3781 agcgctggtc atgcccgtgc cctccgaaat tattccaatt ttcgaacata cgaatggtgc
     3841 gagagcatgt caaccggtac cgagtagact tttttactcc gaagctgaag cggtaacctc
     3901 tgtagccgat cctgaatgtt gaacgttgaa tgattgaacc atttcttatt taaccctcat
     3961 ccccttccaa gttccggaac cggtcttccc ttccatcaca ttgccattga cccgacctgt
     4021 ataatgattg ccgccggcgg taaaaaggat ctgatcaccg atcatcttgc cctgcgtgat
     4081 ctccgctatg ctgttgccgg ttttgatcgt gccggaaagc atctggaagt tttgcttcaa
     4141 actcaactct ccctgcggca attgccagct tccttccact ttcgcgggaa cgatccacag
     4201 cagcgcggtg caccagtgat cgcactcccc tcccaccgta gcgctttcgt ccgcttccca
     4261 ctcgcccatc gtaaacgagt tcgagacgat gcgggtgccg ggtttgagat cgagaatctt
     4321 cggccggagc tttaagttga tctgcggcag aagaaacatg gtgatgacct gggcatccga
     4381 gaaatcgctt tcgaatagat cggctttggc aaacgtagct ttgtcgctca cacccgcctt
     4441 ggcagcgttg ctcttcgaca gctcgaccat atcggggttg tattcgatgc catgggcgcg
     4501 caggccacgt ttggccgccg tgatcaccgt tcgtccatcg cccgagccga ggtcgattag
     4561 ataatccttg ggcgttactt tggccatatc gagcatcttg tcgactaact gttgcggggt
     4621 cggcacccac accacatcct tcccttgttg gccgacccgc ggctcgtaac cttgcctatt
     4681 gggctgcgac tgagcgccag cacaggcgcc gaaaacggaa acgcaaagcg ccaacatcaa
     4741 cgggcgcatt cttcgcgaca gattcataac catctcctgt aattcaattg attgctgcgg
     4801 gggccgaaca gttttatttg ccggcgcggg tggccttgaa agtgccgccg gtcttcgagc
     4861 ttccctcgat gctgctaccg ctgatccgac cttgatattc cgtgcctcca gcggtgaatc
     4921 tgatctgatc gccggtgacc ttgccgttga tcggcccggt gttgttgccg gtcttcaaga
     4981 cgcccgaaac catctggaac gtttgcttca agacgagctc tccttgaggc aactgccagg
     5041 tgccgtccac cttggctggc acgatccaga gatacgccgt gcaataggaa gcacagccat
     5101 ttttgacatt cgcagtctca tcggcttgcc attcgcccat agtaaacgaa ttcgaaacga
     5161 ttcgagtgcc gggtctcaga ttgagcagct tgggacgtaa tttcaaattg atatcgggca
     5221 acaaaaacat tgtgatgact tgcgcctgag agagatcggt ttcgaacaaa tccgctttca
     5281 tgaaactggc ccgatcgctg acactctcct tcgacgcatt acgcttggag agctcgacca
     5341 tgtcaggatt gtattcaatg ccaagcgcct tggcgccgcg tttggcggca gcgatcaccg
     5401 tgcgcccatc gcccgagccg agatcgatta caaaatcctt ggacgttacc ttcgccatat
     5461 cgagcatctt ttccaccagc tcctgcggcg tcggcaccca aatcacgtct ttgccttcct
     5521 ggccgacctc gggtttaaac tcctgcttgg ccggctgcgc ctgcgccgcc ggacctgcgc
     5581 tcagcgcggc aataaacagc gccaaaaaca atcgacgaca aatatgcgat gcgttcatct
     5641 gcgctcctat tttgtctgac gaattcgaac atcgagagtc gcacgcgcgg aagtaaatgt
     5701 ccagtttgac tcagtccaaa cagccgtcgc ggaggcgaca gcgcttgcgc ctgtttgatt
     5761 ccgctataca ttcgatataa aaaattctgc gacggcacac gagtcatcca ttcagagaca
     5821 gagcgactga taggaggatt ttcaaatgcg gcaacgatcc gattacccga ttttttccga
     5881 ccgggagatc gccacaagac atcaggcggt ttatcgactc atggaacagg agaatgtcga
     5941 tgcgcttctc atctacggcg ctgggcgcta cgcctcggac gtttattggt tgagtgattg
     6001 gccgagcagc cgcgaagctt acgtgctttt tcaaacgggt aaagaaccgg ttattctgct
     6061 gcaattgttt aatcactttc caatggcgaa agtgatgtcc gtgattaaag acgtgcgctg
     6121 ggccggcgcc aacactacca gtagcgtcct cgatctgatt cgcgagcgcg gtttggattc
     6181 caagaagatc ggcatcgttg gttcactccc ctatcaggtt tatcttagaa tcgccgagaa
     6241 atttcccggc gccgcattga gcgatcttag cggcaaattg cggatgatgc ggaccatcag
     6301 gagcgcggag gaaatcgagc gcatccgctt tgcttccaag ctcaccgatg aatcgattga
     6361 agcgctcgcc ggcggcctca aggcgggcat gcgcgaggac gagattgccg cgctgattga
     6421 gccggcttat ctaaaaagag gtggttacgc gggaattcat tttatgagca gcatgccaat
     6481 gcgcgacccg gatttcccgg tgccggcgca gttccaatcg agccgcaaac tgcgaaaagg
     6541 cgattgcctg atcactgaga tcagcggcgc atattcgggt tacagcggac agattcaccg
     6601 gaccttttcc gtcggcgaag gaccgacgcc ggaatggcaa caaatgcatg ccgccgcagt
     6661 tgaggcgttc gaaatcctgt cggaggtgat caaggatggc gccaccacca ccgaagcaga
     6721 agaggctgcc gagatcattc atcggcgcgg ctacagcacc tatgatgatc tggtccatgg
     6781 cgtaaatcag tatcccccga tctttcaaac caaaacccgc aagcgtcatg attccaagga
     6841 aatggtgttt cgcgaaaaca tggtcattgt catccagccc aaccttatta ccgaggatga
     6901 aaaaatgggg ctgcaattcg gcgaaacttt gctggtcaca aaaaaaggat gcgagacttt
     6961 gaatgcctac ccgagggaat gggttatctg ccagggatag tacaaataat tctctggata
     7021 ctagacggag actgtcgagg aagaaaggcg aggctggaag gggcgaccgc agcccgacgt
     7081 gaaggaccat tgactttttt gagtgcttga cgagggtttg aaaatcctct cgggcggccg
     7141 ttgtaccggc gcggcgcgct gaacacctca agccgcaaag ctatcgccgg cgcgttggaa
     7201 gcctcacctc tccgcagcaa taaaacacga atactccggc taccgcacca gcgatatcgc
     7261 aattgttccg gcaacgacgc acaacgcgcc aataatcgtt cgcacggtca aacgctcgat
     7321 gccgcgcagg aacagccagg tgcccaatag aatccacaag gggctggtgg cgacgatcgg
     7381 cgagacgatg accaccgcgc cgacgctcag agcggcaatc actaaaacga ttcccaacgt
     7441 ttcgaacacg ccggcaaaca aaaacgcgcc gatcgattcg cggttccaga ccggttgctc
     7501 tgcccctttt ctgggcaaaa ctacaaagct agccagccag gggagcgcaa ccacgccgat
     7561 aaccgcggcg agatacaagg gttcgttggc cagactcaac gcataacggc gcagcggatg
     7621 gctgattccg gcgaggaaag cggcgccgag aggataacca atataccacc agcgaaaacc
     7681 ggcgcgttgc gcttcggttt gccatgagat caatgtgatc ccgccgacga tcaggaccgt
     7741 gccgccgatt accggcagag tcgcttgctc gccgagaaaa ataattgcca gagctgtgct
     7801 aaacagtgga tgcgcgccgc gcacggtcgt gccccgggac gcgccgatgt aatggatacc
     7861 ggcgtaggtg aacagccgga taatcggctg aagcgtgccg gtcacagcga aaaggaacag
     7921 cacccaccag gataccgtcg gaatcccgcc ggtcagcaac acggcggcgg acaaagtcac
     7981 ggcatgaatc gtgaccgaga ccagtgtgac ggtgatcggg gtagagtagc gtaagccgcg
     8041 tttggcggcg attcccgaag cggcgtagga aagcgccgcc gccagggcga tgatttgagc
     8101 tggaacatcg aaccacatga agctgcgaac ggcgccaata gcgcagacta cttctcaatt
     8161 tcccgacgaa tggcgtccgc gaattgactg gtgctggccg ttccgccaag atcctgggtc
     8221 agacactcgc ctcgcatcag cacggcgtgc agcgccgcgc ggaggcgggc cgccgccttg
     8281 gcttccgaaa tatagtcgag catcatgacc gcggccaata tgattgcagt cggattggcg
     8341 atgttttttc ctttgatgtc cggtgcgctg ccgtgaacac tttcaaaaag cgcgtgatta
     8401 tcgccaagat tggcgccggg aactactcca aggccgccga ccaagccggc cgcgagatcc
     8461 gagacgatgt cgccgtagag attttccagc aatagaacat cgaggcgagt cggatccaaa
     8521 accaattgca ggcaggcgtt atcgacgata agatcgctga aggcgatctt cttgtatttt
     8581 tgcgccatgt ttctggcgca ttcgagaaaa aggccgtcgg tgagcttcat gatgttggcc
     8641 ttgtgcaccg cggcgatctt ttttcggccg ttggcaacag cataatcaaa agcaaacttg
     8701 gcaatacggg tcgaggcctt ttcggtgatg attttcagac tttccatgac acccggcacc
     8761 acttcgtgct cgatgcccga gtattggccc tcggtgtttt cgcgcaccac gatcaagtcg
     8821 acattttcat agcgtgcttt aacgccggga agatttttga ccggcctcag attggcgtag
     8881 aggtcgaaac tctggcgcag ttcgacgttg acgctggcaa agccctcgcc caccggcgtt
     8941 gtcactggtc ccttaagagc gactcggttt ttggcaaacg aatcgatcat ttgcttcggt
     9001 aaggtggtac catgtttttt caatgcgccg gcgcccgcca aatgactgtc ccattcgatc
     9061 ttgactcccg tggcttcaat gagtttcaaa gtgggtttga cgacttcggg gccgattcca
     9121 tcgccgggta ttaatgtgat tttatgtctt ttcactcgca ggtccttcca catcatgaat
     9181 gctgcagcag caatcgccta tttatacacg atcgaccgtt aaatagcagg ctatcgtcac
     9241 actccgcccc ggcgttgact ttatggttca ccgtcagcta gcctgatgca ttaaacagag
     9301 atgcaagaca gagtaccccg cacgcggctg ctctccggca cgatgcgctg ccgaatagag
     9361 cctcctgaaa aagtctcata gacttcgttg gcttgttcgc attagattaa tcgatgctcc
     9421 gaaaacttcg tgaccggctg gccggtcttt tttttggctg gcgcatggtc ggcctgagct
     9481 gcgcgatccg cgttcttggc ggcggacttc acgcgtatgg ttttactgtt ttttttctgc
     9541 ccattactca agagttagga ttgtcacgcg cggcgacttc gttggttttc tctttggcgc
     9601 gcgctcaagg cgccattgaa gggccggtcg ccggctattg catcgaccgg tacggaccgc
     9661 ggccggtgat tttcgtcgca actctgttag caggcatcgg ctacatgctt ctggcggcgg
     9721 ttcacagtta taccgctctg ctgatcgtct atatggggat catctcgctc tcctaccaag
     9781 ctggatttat ggatgcgacc atgatggttg ccaacagctg gtttatccgc aaacgagcgc
     9841 tagccatgtc gctcaccagc gggtcgatcg cgcttggcgg cactctggtc acaccgtttc
     9901 tcgcctatgc cgtgcatgca tggggctggc gccgcgccgc gatcggcgcc ggagcggcgt
     9961 ttctaatcgg cggcctgccg ttgtccctgc ttttgcgccg ctcgccggag agcatgggct
    10021 tattgccgga tggcgagccg ttgcccaaag cgctcgacgc aacaggatcg acccacgccg
    10081 tcgaagaagc aaatttttct ctgcagcaaa cattgcgctt gcgcccgttc tggctgctgt
    10141 cgtttgccac cacattccgc gtcgtttgtt ccagctgtat cttggtccat ttcgtgccga
    10201 tcctagtctg gcgcggtttg accgagcagc gcgccgcctt ttttctagca ttcatcgctc
    10261 tgctcggtat gccggcacat ctcctgatcg gctggctcgg cgatcgcctg gataagccga
    10321 agctcatgtc cgtatgcatg atgattgcaa gcttgtcggt gtacttgctg ttttcatatg
    10381 cggcgcagtg gcaactctgg ctggcgctgg tgctcttcac cgtcatcgaa ggactttttc
    10441 ccgttacctg ggccacggtc ggtgattttt ttggccggaa caatttcgcc aaagttcgcg
    10501 gcagcatgag cttcctttac atgtggggta gcgtgatcgg tccggtgatc gccggcgctg
    10561 tgtacgatcg cacgcaaagc tacaggccgc tgatgtgggg actgatcagt ttgtgttgga
    10621 taacagcgct cctctatgct gtactggcaa agccgatcgc gctttcttct gagccggtca
    10681 acctgtgaat cgattgagca agcacaccgg taaaacattt atcaaccgat ggcttccggt
    10741 gctcctttgg gcgaccgtga tctttttatt ttccaccgag cactttgcag cgccgcaatc
    10801 ttcccgaatt ctggggccgc tgttgcgctc ggcgtttcca ggtattaccg cggaacaagt
    10861 gtcgctggtg caatttgtcg ttcgcaagct cgcccattgg tctgaatact ttatcctagc
    10921 cgtattgctc taccgagctt ggtatgccga gaacagcggc agaagcccag ttcgccctgc
    10981 cgcattgaca gtgtgttttg cgctggtctg ggcgatcagc gacgagtttc atcagtctct
    11041 ggtgccaagt cgaaccgcga gtcttgtcga cgttatgatc gacggcttcg gggcactttg
    11101 tggaagtttt tggatgtacc tgcgtcatag atgagccata gacccaagac taagccgtcg
    11161 cgtatggtat ctacggcgtt aaaaaacttg acaatcaaac cggaactatc cacgatgctc
    11221 agcacggtga ttttgccgga aaaccctgct tagacactca tattccatcc cctcaatgac
    11281 attttttaaa cgtctgaccg gcctgctcat tcttcttttc ctttcgtcag cttgctcgag
    11341 ctccgacaac ggaccggcag ttactgcctc aaacttcatg ccggcggaac tcctcaaggg
    11401 agccgcatac tcgccctcgg cgatggaggc gttgagccga ggccttgcgg ttatcacacc
    11461 cccgggagcg gcgttgcagg atgtttatta cgaattcgat agcatcgatc tgaaagccga
    11521 tgcagaggcg atccttaaga agaacgcgga atggatgaaa gccaatccgt tggcccgcgt
    11581 tgaagtggaa ggacactgcg acgatgtcgg atcagccgag tataacctgg cgctgggggc
    11641 taaacgcgct caaacggcca gggattttct cgtgcagcaa ggtatcgcgc ccgatcgact
    11701 ggttacaatc agttacggca aagaagcgcc ggcgtgtttg gagcagaacg aagactgtcg
    11761 ggttaaaaac cggcgcgccc gtttcgtgat gtttaccgag ctgccgactt cataatccca
    11821 aaatgtcaga agacttttca ccggaagagg cgcaggcaat ccagaggttc gtctccaaaa
    11881 ctccggcagc gcctccatct cgcataatcg gatcttaaca ccgcagagac acagagtgcg
    11941 cagagattga gattcctgat tcgttcactc gtaaccaata gcggagacaa tcggggtggc
    12001 agcggcttgt ttggccggcc aatagccggc cagcaagcag agaatcacag atacgaagat
    12061 ggctagagct gccaggtcat agggatagta gtattcaaaa gtccaaccgg ttagcacctt
    12121 cgtgttatac accacgtggt gataggacat gatcgtgccg gcgatcaaac ccaagaagcc
    12181 gccgatgagc cccatccagc ctgcctcaaa gatgattatg cggcgaatct gagcctgggt
    12241 cgcgccgatc gcgcgcaata cgccgatctg tcgcgcgcga tccagaactg acgctaacag
    12301 cgtgttgatg acactaaaaa ccgccacaat caccgcgaca atttcgaccg catagttgac
    12361 cacgaacgat tgctccatga tacgcaccac cgactccttg agctggcgat gggtgctgat
    12421 gaaaagctga tactgctcgc cgtaatcttt cttgatctgc tcaatgacga tgttttgatc
    12481 ggcgcccggt tccaaccaca ggtcgaaagc atccaccaac tcatcctgcc aaaatttttt
    12541 gtacaacgcg cgatcgatca aaacgctgcc gatatcggac gaataatcga tgtaaacgcc
    12601 cagcactctg aaggcaacga gaccggcggg agtggcaagc tcaacggtat cgttggcgcc
    12661 tttgccgaac ttgctttgaa agctctcgct aacgataacg cctttgccgg cggccatttc
    12721 gctcaacact ttcttcccct ctcctgacgg tgtgggcagc ttgcgcacgg cggccgaggc
    12781 cgccgctgaa aatgattcga tcagtatcgg ccttccctga taattggagc gaatcaaacg
    12841 atagagatcg acgatcttga cgccggagac ggatctgagg ccctgcaatg gagcttccgt
    12901 gagcggcaca tttttcggac ccgcggtgcg cgcaccggag ctgacgatca gatcggcagt
    12961 gaccatttga tcgatccacg cgagcaagga tccacggacg ctgttaacga acgcggcgat
    13021 ggtaaagatt gcggcaaggc tgatcaccat cgttgcgacc gtgatgccag accgtatcgg
    13081 gttttttcgc aaactatccg aggccagcct gctttccgcc caggaaaatc cgggaatggt
    13141 tatgccagcg cgcccgaacc agttaacgca ggagctgata atcagaggac aaagaaatgc
    13201 cagtgcaagc aagaagatga gcatgccgat cacaccggcg ctaaactgct gcacggggct
    13261 ccgcagtagc gcggcaaaga aaataatcat cggcgacagc agaaagcaaa ggagaccaag
    13321 ccgggtcccc caagatttgc taccatgaaa taccggacgc cagactgatt gccgggcatt
    13381 ttccaacggg ctgatgcgaa ccgcttccac cgccggatga agagcggcga gcacggccac
    13441 tcccacgccg ctggccaggg ccaacagcaa ttcctgccag gtcaaaccgg cggtcatcag
    13501 accgatcgac gaaaacagat tgctgacggt ttcgccgacc gctaccagtg ccgcctgcgc
    13561 cagcaaccat ccaaacacgc agcccgccag cgctcccacc agagccagga gcagcgcctc
    13621 taagataatc catcgaatca actcgccgcg gcgcatgccg atacagcgca aagtgccgat
    13681 ttccctcttt ctctgcacaa ccgagaccga cacggtattg taaatcagaa agaatccgac
    13741 aaagagcgca atcaggctca caaagaaaag accgacccga aaagaggtca gcaacgattc
    13801 gatctgctcg cctcgtttgc gcggtcgttc gacatcggcc atgccttgga ggctctgccg
    13861 tagtctctgc tgcacatcgg caacgtttgc gcccggctcc acggtaagat cgacgatgtc
    13921 gagttttccc tgcttgccga atgccctctg cgccactggc agatccatca acgcgaagtt
    13981 gccgccgaat accttagcgg ttccttcctc cttcaataag gcgcgcaccg tgtaggtctg
    14041 cttgccgcgg ctggtgatca cggtgagctc ggaccccaaa ttgagtgcga gacggccggc
    14101 aaacgtttca gtgacggcga tcgagtcagg agcggcaatg aaatcaagcg cccggtcgaa
    14161 ctcgaacccg gcgccggcaa accgatgatc ccgaatggca aaatcggtca ataagtccac
    14221 gccataaaca taaaggcgtt cagccgcggc gccgctcacc ggcaagaagc cgtccacggc
    14281 cgcggccgca tcctggacgc cgggcgtatc gcgaattacc ctaaagagat cttcgcgaat
    14341 gccgctttca ccatttgcaa cttgcagcac agccttgccg gcaatctgat cgatggtcga
    14401 ttgaaacgat gtggtcagcg atctattgac gatggcgata gcaacgatga cagcgacacc
    14461 taaagcgatg ccgagaatcg tcagaagagt tctcagccga tgcagtttga gatggcgcca
    14521 cgacacggtc tcaagcaaac gcaacatcag aagtctttct cgatcaggcc gtcacgcagg
    14581 caaatggtcc ggtcgccgac cttggcagcc tcggggctat gcgtaaccat gacgacggta
    14641 cagcgatcat cctgattgat gcgccgcaac aggttgagaa cgatctcacc attcttggaa
    14701 tccaggttgc cagtcggttc gtccgccaga ataatcggcg gctcgaaggc tagagctcga
    14761 gcgatggcca atcgctggat ttcgccaccg gacatggctt caggcaagtg gcttccgcgg
    14821 ggcgacaagc ccaccttgtc gagaagaaac ttcgctagtt g
//
LOCUS       DASUVS010000296        17810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1869322, whole genome shotgun sequence.
ACCESSION   DASUVS010000296 DASUVS010000000
VERSION     DASUVS010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 17810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17810
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869322"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="VF182_16030"
     CDS             <1..1061
                     /locus_tag="VF182_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438647.1"
                     /translation="VLYSGGIPRLINIICDNALLIALGISKKRVSANIVDEVAEDLHI
                     QARPLEAQITSGLTGNKPDQTEAAALSNNENLFRPELLGQRYASRTLAAGFGILLVFV
                     LITGATSILYDQAKILVSRAGAGAGHLFSGDATSPVRVQSSPQRSRDNAEEGWSDFGK
                     LKMEVSRILMKNKERTPDSSATSSAADRSSYFNDPQTTAVPANPIIEDNIAPAPPKRT
                     VAPPDHVIAARTIEPADRAIQLQPDRQLPRSRGPQYWPSTRTEAKPPRADREQRFFQG
                     VFEVTADSVIFEEPRMESALVKTLTPGKQVRVEQKRGNFLMVRSLNDPDIAGYVHLDH
                     AYFRRIRQDANQVKALQR"
     gene            complement(1253..2008)
                     /locus_tag="VF182_16035"
     CDS             complement(1253..2008)
                     /locus_tag="VF182_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011645909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEX6438648.1"
                     /translation="MMTLEDFAGAIRESRKIVVFTGAGISTESGIPDFRSPGGIWSRY
                     RPVSFQEFISSEACRVEAWKRRVETYRVDKNAKPNIGHYLVQSLNEKGKLIGLITQNV
                     DGLHSQSGLPEDKIVELHGSNRIIRCLDCSKSYDPAIIIERLTGDFSSPRCDACGGIL
                     KSATISFGQAMPERAMRQAQEWTERARIFIVMGSSLQVQPAASFPVIARRNGALLAIV
                     NREPTSLDGYASFAHHGAIGEFCRQLNTMIADG"
     gene            complement(2051..3238)
                     /locus_tag="VF182_16040"
     CDS             complement(2051..3238)
                     /locus_tag="VF182_16040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6438649.1"
                     /translation="MSGESKNFLKIVQPVFFLVGIFLLNFTARIILSPLLPTIEEELA
                     ISHAQAGFFFFLISAGYLLGLLSSGFFASRATHKRTIVASSAGVGLALIAIAFASHLS
                     TIRVGLLALGFAAGLYIPSAIAAITSVIRQEHWGKAIAVHELAPNLAFFASPFIAEAF
                     LIWASWRVALGSLGAMSFVASLIYHYYGRGGEFTGESPGSRAFGTLIRAPAFWLMLIL
                     FGLGVSSTIGVYAMLPLYLVNEQAMRPSWANTVVALSRMYGPILGLLGGWVSDWLGPK
                     RTMIVSLIFTGIATLLLGVVSGWWTSAVVLLQPLVAVWFFPAAFAALALITGPNARNL
                     AVAFTVPFGFLLGGGAIPTFIGMMGDAGLFAMGFVIAGSLITCGGILAMVLHLPQSAK
                     KAG"
     gene            complement(3451..3894)
                     /locus_tag="VF182_16045"
     CDS             complement(3451..3894)
                     /locus_tag="VF182_16045"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX6438650.1"
                     /translation="MELVTWTPFESFNKLQSRINGLFDGNFAPSWPDTATGRTWYPAV
                     DILESKDAYLLRAELPGMNKEDFNLELKEGTLTLTGERKFTKPAEGVQYRHVERANGK
                     FVRSFTLPETVKHDGIEAAYKDGILEVHIPKAEEAKPRQIQVSVH"
     gene            complement(4033..5358)
                     /locus_tag="VF182_16050"
     CDS             complement(4033..5358)
                     /locus_tag="VF182_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HEX6438651.1"
                     /translation="MMFVRAQFMIVVGMFACAGLTWWTGLAWADLDEYIRKPEPNFAW
                     KQNGQTRERSGDQIFDLHFVSQTWQGGNWQHQLQVYRPQSVAPAAMMFLWVTGGSATP
                     EQIAFGMELARKSKAPVAVLYDIPNQPLLEGKLVEDDLIAETFVRYLKSKDENWPLLF
                     PMVKSVVKAMDVLQAFGKGEWGEPVSKFIVGGASKRGWTTWLGAAVDQRIGAIAPVVI
                     DTLNMRAQMPRQLKAFGGYSARLQPYTSRGLVPIPDSPEGERLLSMVDPWAYRARLQL
                     PKLIVNGTNDFYWATDALNLYWNELPANKWVIYVPNAGHDLRRRDQGNSDQRADLING
                     LAAFSRHQINRRPMPEVSWKHEDSAGKLRLSIAANPQPLGARLWVAQTDSKDFRTASW
                     TEQKATVSEGIVVGEVTPPEKGHVAFFGELDYEIDGLKYQLSTQIRVTE"
     gene            5587..7164
                     /locus_tag="VF182_16055"
     CDS             5587..7164
                     /locus_tag="VF182_16055"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6438652.1"
                     /translation="MYNSYFGFSVSPFRVTPDPSLFYSNPVYEEAYANLCYGIEAKKG
                     FIVITGEVGTGKTTLLRKLMTHTSEGIIHTVYVFNTYLNFTELLQVIHYDLGLGQKEA
                     NKVKLLQELNDFLIAQLKDGNIVSVLVDEAQNLSDEALEGLRLLSNLETDQEKLIQIV
                     LMGQPELQARLDQPSLRQLKQRVALQCRLLPLRHEEVCRYIDFRLRAAGYTGKRLFHP
                     DAYRQIAVASNGIPRLVNIVCDNALLCAFGRSETTISGDIVKEVAGDLRLTSQTELPR
                     AQNFSRASDALAESDMLTGKTASKPPRSKVRHLARYGLETCLIILGLIIIASFVDPKN
                     FRVNAGRTLENAKGNLNEWALFMSQEPAVTAKANAQVEVETKEQRVIIPQGSSIYKIA
                     SEAYGTKNGLGMDLIKEFNPGIKSLARVAAGRALLLPSLTPETLLRRQGDGSYRLIVA
                     AFQSPTAADEYAQRLRNKGYRVSITPRTVSEDVLLYRIEIDGLQNPEEANHIWLAGLR
                     NDSFGLAGEAARSPLSR"
     gene            7186..7869
                     /locus_tag="VF182_16060"
     CDS             7186..7869
                     /locus_tag="VF182_16060"
                     /inference="COORDINATES: protein motif:HMM:NF028512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438653.1"
                     /translation="MSSVYDALIKAGKKAESAGTLAVSWQRLNLKWKIMLAVFGLLFA
                     VIVNHLVTRVLRDQMEESAMLMTINLSDAAAGYIASNDALRLRTTVTKYARLNRVAYV
                     FVQDREGKIIASSLASVSPELESAVTANPELQVSRREVTLEGKTVYETRAPILDGQLG
                     SAHIGLWAGAMEGDIHQALFKFVWPITLGLLAVAIAIVMLGQSLIQAARRLVELRSAS
                     QTTATQRIK"
     gene            complement(7931..8287)
                     /locus_tag="VF182_16065"
     CDS             complement(7931..8287)
                     /locus_tag="VF182_16065"
                     /inference="COORDINATES: protein motif:HMM:NF026924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm8 family immunity protein"
                     /protein_id="HEX6438654.1"
                     /translation="MIWPEIRRIHSPDLEPPNLPKNPADCEILFQVYVGPKDDEGEEA
                     FSFTVMTASRFIKDAEAQWGRGKLILAVFEWPAVIQAVAELLAQCARPSWGEVVAELN
                     KELIWDFDGYKPADAS"
     gene            complement(8446..9642)
                     /locus_tag="VF182_16070"
     CDS             complement(8446..9642)
                     /locus_tag="VF182_16070"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX6438655.1"
                     /translation="MKVFLSYSSSDRTLVEPIYLALVAQGHTVFFDRAGLPAGEEYDV
                     RIRRALENSHLVLFMVSPDSIAAGSYTLTELAIAQKTWAHPAGRVLPVILRPISLEQI
                     PPYLKSVTFFEPEGDRTASVADQVYRIAQARRRAKLKKVAAMLIVAVILCAGAYRYRM
                     FREPAAEIIGQDGAPALIVPAGNFMMGDDVESPLREIYLDSFYIDKFEVTLSRYAKFL
                     SATGGAKPPEDWQQSDRASTSALPVVGVDWHDADAYCRWADKRLPTESEWEKAARGRD
                     ARNYPWGNEEPSPAHANFGKSVDAPYTGGLAPVEGRPNGKSPYGVYDLAGNASEWVAD
                     WFAAAFARGDVRNPKGPKNGTAKVIRGGGWYDPPERLNASRRMHASPNTRAQDLGFRC
                     AKDVRE"
     gene            complement(9914..11119)
                     /locus_tag="VF182_16075"
     CDS             complement(9914..11119)
                     /locus_tag="VF182_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEX6438656.1"
                     /translation="MKFGIDRLLEEPALRKALKGRRSALLAHPASVTRHLQHSLDALA
                     ACNDVKLDAAFGPQHGLRGDKQDNMIESPHFTDPIHDIPVFSLYGEVRRPTVAMMDSF
                     DILLIDLQDLGCRIYTFVTTLRYMLEAAAERKKTVWVLDRPNPIGRPIEGLRLRDGWE
                     SFVGAGPLPMRHGLTLGELARWFVRALRLDLDCEVVTMDDWQPSTGPGFGWPLGRRSW
                     VNPSPNASSLSMARCYGGTVMLEGTTLSEGRGTTRPLELFGAPDLEPRELLAKMDSLA
                     PQWMRGCRLRDCWFEPTFHKHVRKLCAGIQIHVDDPAYDHNLFQPWRLLALAFKGLRA
                     LRPDYPLWRDFSYEYETERLAIDVINGSELLRRWVDDPAASAADLDDLAKADESSWAE
                     ERDPLLLYR"
     gene            complement(11116..11712)
                     /locus_tag="VF182_16080"
     CDS             complement(11116..11712)
                     /locus_tag="VF182_16080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HEX6438657.1"
                     /translation="MSLIAQEVALACHPETPSQSVRTIRARVSQAPNGGIAIAYFIEG
                     QISRLCLPGRSAPSRIDGLWQHTCFEAFLAVKDAPGYHEFNFAPSGQWAAYGFKSYRE
                     GAPLPQASDPQIARRASANCFELDALIQQASLPAVPVNGRLLLGLSAVIEEQDGVRSY
                     WALKHPPGRPDFHHASAFVLEWPPHVAAIRDPAYTGKR"
     gene            11892..12803
                     /locus_tag="VF182_16085"
     CDS             11892..12803
                     /locus_tag="VF182_16085"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX6438658.1"
                     /translation="MDKVRSLHDAIAQDVADGDSIAMGCGLESLIPFAASHEIIRQRK
                     KNLTLIAPISDMQFDQLIGAGCVKKIIASWVGNVAAGLGHNYRRAAESGIPHPIEIEE
                     HSNFTIGLGLQAAATGLPFMPTKSIRGSDISRCPQFASVKCPFTGDELLAVKAISPDL
                     AILHVQRADRQGNAHAWGNFGVMREAAFAAKKVILTCEEIVDHDVILSDPNRTLIPGF
                     LVSSLVHQPFGSHPSPTQGYARRDDSFYFEYHKETRSRDGFENWLQTWIFSVANHGEF
                     LERLGADRVKRLAPQKTLITEPANFSY"
     gene            12832..13269
                     /locus_tag="VF182_16090"
     CDS             12832..13269
                     /locus_tag="VF182_16090"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX6438659.1"
                     /translation="MKAVPFFKLTQEGADIMVDAAIEKAKELKQIVSIAVVDSGGHLF
                     SFKRMTNGRTHNTMIALAKARSAALTTAATGKTNREGGEIPDHQVLAQTLAVGPGWFI
                     SIEGGIPLTIEGQCIGAVGVSGAPSAVDKQICQAGIDAFNQAT"
     gene            13336..14091
                     /locus_tag="VF182_16095"
     CDS             13336..14091
                     /locus_tag="VF182_16095"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX6438660.1"
                     /translation="MHYTLRELMVAAASREIRDGDKVFVGMRLPLLGFAVAKETHAPN
                     ALGIFENGIIRDWPALESILTMSDPPNVARALSCGGLNDVMHLLQSNRVDLGFIGGAE
                     VDRFGNLNTHWVEENGKRIRLPGSGGAADIATMAKRCIIIMNHERRRFPARVHYITSP
                     GFGDGGDWRSRNGLIGSGPTRVITSMGIFSFDASTHEMTLTANHPGTNIDEIKNETGW
                     PLRIAPELQETAAPSDAELSAIRKYDPKAIWTS"
     gene            complement(14295..14807)
                     /locus_tag="VF182_16100"
     CDS             complement(14295..14807)
                     /locus_tag="VF182_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017941131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6438661.1"
                     /translation="MAEKNPTVTVSTSMGDIRIELDADKAPITTKNFLDYVNDGFYDG
                     LIFHRVIPGFMIQGGGMDPQMKQKSTKSPIKNEATNGLKNKNGTVAMARTNVVDSATA
                     QFFINVKDNDFLDHRSTSADAFGYAVFARVIDGMDVVQKIERVKTRNSGMHQDVPMDP
                     VVINSVRLDG"
     gene            14945..15862
                     /locus_tag="VF182_16105"
     CDS             14945..15862
                     /locus_tag="VF182_16105"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX6438662.1"
                     /translation="MPSDRKMEGILVPMPVAFKNDGAIDQSATDAIIDFYLASGVHGF
                     FPLGTHGQGMVMEIDERKKAAEQIVKRVAGKVPVVMHIGTANTYSSIELAKHAAGLGV
                     DAIGVVPPYYYPHDDYEVYAHYKAIAKSVPGVPLFIYDNTETTHVEITPPKANKIIEA
                     IAPNTLAGIKVSFIPYDKLLGYVFQLPKSVGVFPGGIFSLYSGYSVGVRGAIHPPSTP
                     FPEACVRMYEALKAGNHQEAQKQHDLLAAVLQVVGRFLRTHGRGVFAELMRMRGLPVE
                     RFPRWETPPFTDKDRADLKESLQQAGLKL"
     gene            16237..16776
                     /locus_tag="VF182_16110"
     CDS             16237..16776
                     /locus_tag="VF182_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438663.1"
                     /translation="MNPLLIIAVPLALVILGMILNSTGLSYDQPASSSEQDPIKKLTA
                     EKDAYRKFFDLQRSRSLKRQQRISQYSWLVMAAFIGAFIWLYTDTVNKTSLSSRIAGM
                     QTLATEEGKQMVLSVTLSDGNNVKYLVKLPQAEKLETAPKQGMAVEKVSSWELERLGT
                     ALSVGSSPLPVGMALKISH"
     gene            complement(17161..>17810)
                     /locus_tag="VF182_16115"
     CDS             complement(17161..>17810)
                     /locus_tag="VF182_16115"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX6438664.1"
                     /translation="RPDSGFFASAIAIIFLFLGAGGVVGQLQDSLNTIWRVVPKTGRG
                     VRGFLQDRLISYLMVLSVGFLLLVSLIISAVLAAISGILGGFLTIDAATAYAFDLAFS
                     FAFNTLLFAAIYKFVPDVRIAWRDVWMGAAMTSLLFSAGKFLIAFYLGHSTGTSIYGA
                     AGSVVTLLLWIYYSSLMFFFGAKLTQVYASRYGSKAISVDSPRSAEPGTKDRKST"
BASE COUNT         4226 a         5026 c         4733 g         3825 t
ORIGIN      
        1 tagttctgta ctccggcggt attcctcggc tcatcaatat catttgcgat aatgctctgc
       61 ttatcgcgct gggcatttcc aaaaagagag tctcagccaa tatcgttgat gaagtcgctg
      121 aagatctaca catccaagca cggccgctcg aggcgcagat cacgtccggg ttaacgggta
      181 acaagccgga tcaaacagag gcagccgccc tctcaaacaa tgaaaatctc ttcaggccag
      241 agttgttggg ccagcgttac gcgagcagga cccttgccgc cggtttcgga atactattgg
      301 tttttgttct cataaccggc gctacctcga tcctctatga ccaagctaag attctggtct
      361 caagggcggg cgccggggcc ggacatttat tcagcggcga tgcgactagt ccggttcgag
      421 tgcagtcgag tccgcaacgg agtcgcgata atgctgaaga gggttggagc gacttcggta
      481 agttgaaaat ggaagtaagc cgcattctga tgaaaaacaa agagagaacc cctgattcat
      541 cagccacgag ctctgccgcg gatcgctcaa gctattttaa tgatcctcaa acaaccgctg
      601 ttcccgccaa ccccattatc gaggacaaca tagcgcccgc gccgcctaaa agaaccgttg
      661 caccgcccga ccatgtcatc gcggcgagaa cgatcgagcc ggccgatcgc gcaatccaac
      721 ttcagccgga tcgtcaattg ccgaggtcca gaggtccaca gtattggccg agtacgcgca
      781 ccgaagctaa accaccgaga gcagatcgag aacaacgatt tttccaaggc gtcttcgaag
      841 tcacggcaga ctctgtcatt tttgaggaac ctcggatgga gtcggctttg gtgaaaacat
      901 tgacgcccgg aaagcaggtg cgggtagaac agaagcgagg caactttctt atggtacgtt
      961 cgctcaacga tccagacatc gccgggtacg ttcacttaga ccatgcgtat ttcaggcgca
     1021 tacggcagga cgctaatcag gtcaaggctc tgcaacgatg atactcggaa gctagagctg
     1081 tattgggtac cgtttaggat tcgggaaata ttttcaccgg agagatttgc ggagacgaga
     1141 gagatcgaca atcgtaggcg agatgccggg agccggaaag caggccggtt ttcattcgca
     1201 tccagccttc tatctattta tctctcctga ctcttttcct gccgtgtttc atttatccgt
     1261 ccgctatcat cgtgtttaac tgccggcaaa actctccgat ggcgccatga tgggcgaagc
     1321 tcgcgtagcc atcgagcgag gtcggctccc gattgacgat tgccagaagt gcgccgttgc
     1381 gacgggcgat gactggaaaa gaagcggcgg gctgtacttg cagcgaggag cccatcacga
     1441 taaagatgcg cgcccgctcg gtccactctt gagcctggcg catggcccgt tccggcattg
     1501 cttgaccaaa tgaaatcgtc gccgacttca ggatgccgcc gcaagcgtca catcgtggcg
     1561 aggagaagtc gccggtcaaa cgttcgatga tgatcgctgg atcgtaagac ttactgcagt
     1621 ccagacagcg gataatccgg ttgctgccgt gcaattcgac gattttgtct tcaggcagtc
     1681 cggattgact gtgcaagcca tcaacatttt gcgtgatcag cccgatgagt ttgccttttt
     1741 cgttcagcga ttgaaccaag tagtggccga tattgggctt ggcattcttg tccacccggt
     1801 aggtttccac gcgccgtttc caggcttcga cccgacatgc ttcgctgctg ataaactctt
     1861 gaaaactgac cggccgataa cggctccaga ttccgccggg gctacgaaag tcgggaatcc
     1921 cggactcggt gctaatcccg gcgccggtaa atacaacgat cttacggctc tctctgattg
     1981 ctcccgcgaa gtcttccaga gtcatcataa gaaatttata gaacagccga cgcgcagaaa
     2041 acaacggcgt tcaaccggct tttttggcgg actgcggcag gtgcaggacc atggcgagaa
     2101 taccgccgca agtgatcagg cttccggcaa tgacgaatcc catggcaaac aacccggcgt
     2161 cccccatcat tccaataaat gtcgggatcg cgccgccgcc caacaaaaat ccgaacggca
     2221 cagtaaaagc gactgcaagg ttccgcgcat tgggtccggt aatcagcgcc aacgcagcga
     2281 atgccgccgg aaaaaaccaa accgctacaa gcggctgaag caaaaccacc gcgctggtcc
     2341 accaacctga aacgacgccc agaagcaggg ttgcgattcc agtaaaaatc aggctcacaa
     2401 tcatcgtcct tttaggtccg agccaatccg atacccaccc accgagaagt ccgaggatcg
     2461 gcccatacat gcgcgaaagc gccaccaccg tgttggccca gctcgggcgc atcgcctgct
     2521 cgttcaccag gtacaacggc agcatcgcgt atacgccaat agtcgagctc acgcccagcc
     2581 caaacagaat cagcatgagc caaaatgctg gagcccggat caaggtccca aacgcgcgcg
     2641 aacccgggga ttccccggtg aattcaccgc cgcggccgta atagtgataa atcaggctgg
     2701 ctacgaacga cattgccccg agcgaaccga gcgcgacacg ccagcttgcc cagataagga
     2761 atgcttcggc gatgaaagga ctcgcaaaaa aagccaggtt cggcgcgagc tcatgaacag
     2821 cgatggcttt gccccagtgc tcttgtctta tgaccgaagt gatcgccgcg atcgccgagg
     2881 gaatatagag acccgctgca aatccaagag ccagcaagcc aacgcggata gtcgagagat
     2941 ggctggcaaa agcgatcgca attagagcta agccgacgcc tgcgctggag gcgacgatgg
     3001 ttcgcttgtg ggtcgcgcgc gaggcaaaaa atcccgaact caatagcccc aacaaatagc
     3061 cggctgagat caggaagaag aaaaaaccgg cctgggcatg actgatcgcc agctcttctt
     3121 caatggttgg aagcagcggt gagagaatga ttcgcgcggt gaagttgaga aggaaaatcc
     3181 cgacgagaaa aaataccggc tgcacaatct tcagaaagtt tttcgactca ccactcaatg
     3241 ttgcgcctgg tcgatgctcc ggtgcgaaca cgattcaccg tacttgttac ctcgttcccc
     3301 atcaactcag aaaattttgt tgattacgtc ttacttgcgc gaggctaacg aatgactgac
     3361 acaagcaatt atctgaagag tgtggcaagc gcaaagcaaa aaggggaagc agacgcttcc
     3421 cccttctcta acactaaagc cgcgtgaaga tcagtgtacg ctgacttgga tttgccgcgg
     3481 tttggcctct tcggctttag gaatgtgaac ctcgaggata ccgtccttgt aggcggcctc
     3541 aattccgtca tgctttacgg tctccggcag cgtaaaggat cttacgaatt tcccgttggc
     3601 ccgttccaca tgccggtact gaacgccctc agcgggtttg gtaaacttcc gctcgcccgt
     3661 cagcgtcaac gtgccttcct tcagttcgag attgaaatcc tccttgttca ttcctggaag
     3721 ctcagctcga agcaaatagg cgtctttgct ttccagaata tcgacagcgg gataccaggt
     3781 tctgccggtc gccgtgtcgg gccacgaagg cgcaaaattt ccatcaaaca agccattgat
     3841 ccgcgactgc agcttattga aactctcaaa cggtgtccat gtaaccaatt ccatatgatg
     3901 actcctctct aagcggtgtg atgacaacaa cgtaagaacg gccgcctgga gtgtcaacgg
     3961 cttgacaagc ggcggcgtcg ttcttaactt ctgccgcgca aaacgctcga agtggagccg
     4021 ctggcaaatt cattattccg tcacccgaat ttgggtcgag agttggtatt tcagcccatc
     4081 gatctcgtaa tcgagctcgc caaaaaaagc cacgtggccc ttctccggcg gcgtgacttc
     4141 accgacaacg atcccttcgg agacagtggc cttctgctcg gtccaactag ctgtgcgaaa
     4201 atcctttgag tctgtttgcg caacccaaag gcgcgcgcct aatggctggg gattggcagc
     4261 gatgcttaac cgtagcttgc cggcgctatc ttcatgcttc cagctcacct ccggcatcgg
     4321 ccgccggttg atttgatgac gcgaaaaagc ggctaagccg ttaatcagat cggcccgttg
     4381 atccgaatta ccttgatccc gtcgtcgcaa atcatggccg gcattcggaa cataaatcac
     4441 ccacttgttg gccggcagct cattccaata aagattgagc gcgtcggttg cccagtaaaa
     4501 atcgttggtg ccattgacaa tcaacttggg caactgcagc cgcgcacggt aggcccacgg
     4561 gtccaccatg ctcaaaaggc gctcaccctc cggcgagtcc ggaatcggca ccaagccacg
     4621 gctggtgtaa ggttgcagcc gggcgctata cccgccgaac gctttcaatt gccgcggcat
     4681 ctgcgcccgc atgttgagcg tatcgatcac taccggagcg atggcgccga ttcgctgatc
     4741 gaccgcagcg ccaagccagg tcgtccagcc gcgcttggag gctccgccga caatgaactt
     4801 gctcaccggc tcgccccatt cacctttgcc gaatgcctgc aagacatcca tcgctttaac
     4861 cacgctctta accatcggaa acagtagcgg ccagttttca tccttgctct tgagataacg
     4921 cacgaacgtc tcggcgatca gatcatcctc gacaagctta ccttccaaaa gtggctgatt
     4981 aggaatgtca tagagaacgg ccacaggagc cttgctcttg cgcgccagct ccataccaaa
     5041 tgcaatctgc tccggtgtgg ccgaaccgcc ggtcacccaa agaaacatca tcgcggcggg
     5101 tgcaaccgac tgcggccgat agacttgcag ctggtgctgc caattcccgc cttgccaggt
     5161 ttgggatacg aaatggagat caaagatttg atctcccgac cgctcgcgag tttggccgtt
     5221 ctgcttccaa gcaaaattag gctccggctt tcggatgtat tcatccagat cagcccatgc
     5281 taggccagtc caccaggtca atcccgcaca agcaaacatg ccaacgacaa tcatgaattg
     5341 agctctgaca aacatcatgt tactcctggc ccggatcatc gcccgagaat gatctcattg
     5401 taattatctc tacggtcgca gcttcgcgca gctcgcacaa gtcccggatt ataaccgaat
     5461 ttcgtcttga ctcttgtttt gcctttgatc taaggtttgg ccagcgttta cgctcgcaat
     5521 ggactgattc cacttcggta aacctacgac ctcgatcagc cactgcaaaa ggacggtcta
     5581 ggtacgatgt acaatagcta ctttggattt tcggtctctc cttttcgtgt cacccccgat
     5641 ccgagtttat tctatagcaa tccggtttac gaagaagcct acgctaatct atgctatggc
     5701 atcgaggcga aaaaaggttt catcgtcatc actggtgaag tgggaacagg gaagaccacg
     5761 ttgttgcgaa aactgatgac gcacacctct gaaggaatca tccacacggt ttatgttttt
     5821 aacacctact tgaattttac cgaactgctg caggtgattc actacgatct tggcctgggt
     5881 cagaaggaag caaacaaggt aaagctgctt caggagttga acgattttct gatcgcgcag
     5941 ctcaaggacg gcaatatcgt ctcggtcctg gtggacgagg cccaaaatct aagcgatgaa
     6001 gctctcgaag gtctccggct tctttcgaat ttggaaaccg atcaggagaa gctgatccaa
     6061 attgtgctga tgggccagcc cgagcttcag gcaagactcg atcaacctag cctgcggcag
     6121 ctcaaacagc gtgtggcgct gcaatgccgc ctgcttccac tcaggcacga ggaggtatgt
     6181 cgttacatcg actttcgact tagggccgcc ggctatacgg ggaagcgtct attccatcct
     6241 gatgcctatc ggcaaattgc cgtcgcatcg aatggaattc ccaggctggt gaacatcgtc
     6301 tgtgataatg ccctgctgtg cgcctttggt cgatctgaga caaccatttc cggcgatatc
     6361 gtcaaggagg tggccggcga tcttaggcta acatcacaaa cggagctccc tcgggcgcag
     6421 aatttttctc gcgcaagcga cgccctcgcg gagtctgaca tgctcactgg caaaacggcg
     6481 agcaagccgc cgcgcagtaa ggtgaggcac ctggcgagat acggcctgga gacctgcttg
     6541 atcatccttg gtttgatcat tatcgcctcg ttcgttgatc cgaagaactt ccgggtaaac
     6601 gccgggagaa ctctcgaaaa tgccaaaggc aatctaaacg aatgggctct gttcatgagt
     6661 caggagccag cggtgacggc gaaggcgaat gcacaagtgg aggtcgaaac caaagagcag
     6721 cgagtgatca ttccgcaggg ttccagcatc tataaaatag ccagcgaagc ttacggaact
     6781 aagaacggcc tcggcatgga tttgatcaaa gagttcaatc ccgggatcaa aagcctcgcc
     6841 cgggtggcag ccggccgggc ccttttgttg ccttcactta caccagagac attgctgcgc
     6901 cggcagggcg acgggtcgta tcgtctcatt gtcgccgcgt ttcagagtcc cacagcagcc
     6961 gatgaatacg ctcagcgtct acggaacaaa ggttatcgag tctccattac accgagaacg
     7021 gtttcggaag acgtcttgct ttataggatt gagatcgacg gtttgcaaaa tccggaggaa
     7081 gccaatcaca tctggctggc gggcctacga aacgactcgt ttggtttagc cggtgaggcg
     7141 gcccgcagtc cgctaagcag gtagacagct acatcgcggt gacttatgag cagcgtatat
     7201 gacgcactga taaaagcggg gaagaaggcc gagagcgcgg gcaccttagc ggtctcatgg
     7261 cagcgtctaa acctcaaatg gaaaatcatg ctggcggttt tcgggcttct ctttgcggtg
     7321 attgtgaatc acctcgtgac gcgcgtgctg cgggatcaaa tggaggaaag cgcgatgctg
     7381 atgacaatca acttgagtga tgcggccgca ggctacatcg ccagcaacga tgcgctccgc
     7441 ctcagaacca ccgtcaccaa gtatgcccgc ttgaacagag ttgcgtatgt attcgttcaa
     7501 gaccgtgagg gaaagatcat cgccagcagc ctggcaagcg tctccccaga gctcgaatca
     7561 gcagttactg caaacccgga gctccaagtc agtcggcgag aagtcacctt ggagggcaaa
     7621 acggtttacg agacccgcgc gccgattctc gacgggcagc ttgggagcgc gcacatcgga
     7681 ctttgggcag gcgcaatgga aggagacatt caccaggctc tatttaagtt tgtctggccg
     7741 attaccttgg gacttcttgc ggttgcgatc gcaatcgtga tgttggggca atcgctaatt
     7801 caagcggcgc gccggctggt tgagttgcga tcagcttcgc agacaaccgc gacccagcgg
     7861 atcaagtaac cttattcggc aacaatcccg cacttcaaat cctgacaggc gaagtaaagt
     7921 gtcttctatt ttacgatgca tcggcaggtt tatagccgtc aaaatcccaa atcaattcct
     7981 tgttgagctc cgccacgact tcgccccacg acggacgggc gcactgcgcc agcagctctg
     8041 ccaccgcctg aatgacagcg ggccactcaa agaccgctag aatcagcttg cccctgcccc
     8101 attgtgcttc agcatctttg atgaaccggc tggccgtcat caccgtgaag ctgaaagctt
     8161 cttccccttc atcgtctttg ggcccgacat agacctgaaa cagaatttcg cagtccgccg
     8221 ggttttttgg caggtttggc ggctcgaggt cagggctgtg aatgcggcgt atttcgggcc
     8281 agatcatgtt ttcttttatg ctcctttggc gtgatcggcg gttcgcagat cagatagcga
     8341 gctatgcgat atcgctcgaa ccccagccgg ggctgagccg atccgacttt atcgccgttg
     8401 cgccagcact ctaaactgtt ttgtcacaaa tgtttagcgc aacctttact cgcggacatc
     8461 tttcgcgcaa cgaaaaccaa ggtcctgagc gcgagtgttc gggctggcat gcatgcgccg
     8521 gctcgcattc agccgctctg gaggatcgta ccatcctccg ccccggatca ccttggccgt
     8581 gccattctta ggccccttgg gatttctcac atcgccgcgc gcaaatgccg cggcaaacca
     8641 atccgctacc cactcggaag cattgccggc cagatcgtag acaccgtacg ggctcttacc
     8701 gttcgggcga ccttcgaccg gcgcgaggcc tccggtgtac ggagcgtcaa cagatttacc
     8761 gaaattggcg tgtgcgggag agggctcttc attgccccag ggataattgc gggcatctcg
     8821 gccgcgtgcg gccttctccc attccgattc cgtaggcagg cgcttgtccg cccaccggca
     8881 gtaggcgtca gcatcatgcc aatccacccc cacgaccggc aatgcactgg tacttgcccg
     8941 gtccgattgc tgccaatctt ccggcggctt tgcaccgccg gttgccgata agaattttgc
     9001 gtaacgcgaa agcgtcacct caaatttatc gatatagaaa ctgtccagat aaatctctcg
     9061 aagtggcgac tcgacgtcgt cgcccatcat aaaattgccg gctggaacga tgagcgcggg
     9121 ggcgccgtct tggccgataa tttccgctgc cggctctcga aacatccgat agcggtacgc
     9181 gccggcgcac aaaatgacgg cgacgatcaa catcgcagcc acttttttca atttagcgcg
     9241 gcggcgggcc tgggcaatcc gataaacttg gtctgcgaca cttgcggtgc gatctccttc
     9301 aggttcgaag aatgtgacgg atttcagata gggcgggatc tgctccagcg aaatcggacg
     9361 caatatcacc ggcaacaccc ttccggccgg atgcgcccag gttttttgcg caatcgccag
     9421 ttcagttagc gtgtaactgc cggcggcgat cgagtccggg ctcaccatga acaacaccag
     9481 atgtgagttc tccagggccc gccggattct cacatcatat tcttcccccg ccggcagccc
     9541 ggcgcgatcg aagaataccg tatgaccttg cgcaaccaag gcaagataga tcggctcaac
     9601 cagtgttcga tcactggatg aatagctcag gaatactttc ataaaaagaa gtataactgg
     9661 ggacggggat actcatggta aggtgatttg ccgccgctgc cgccaatcat cagcagctcg
     9721 ggttcaggta agcagatgct tctgatgatc ttgccggcat cagattcaat tcgggtacac
     9781 aagcgcggca aaaataacct ccatccaacg actcggcctt cattggatta tcaacaaccc
     9841 aggacatcca catcagtaat gaggtatgag cttcggcggc agcaacggat agacatcggc
     9901 gctaatcgat cgcctagcgg tacagcaaca gagggtcccg ctcttctgcc cacgaactct
     9961 catccgcttt tgcgaggtcg tcaagatcgg ccgccgaggc ggcgggatca tccacccagc
    10021 ggcgcaataa ttcgctcccg ttgataacgt cgatggcaag acgttcggtt tcatactcgt
    10081 aggagaaatc gcgccagagc ggataatccg gccggagagc tcgcaagcct ttaaaagcca
    10141 gcgctaacag gcgccagggt tgaaacagat tgtgatcgta tgccggatcg tcgacgtgga
    10201 tctgaatacc ggcgcacaat ttgcggacat gtttatgaaa tgttggctcg aaccagcaat
    10261 cacgcagcct gcacccgcgc atccattgcg gcgcgagcga gtccatcttt gccaggagct
    10321 cgcggggttc gagatccggc gcgccgaaaa gctccaacgg ccgtgtcgtg ccgcgcccct
    10381 cggacagagt cgtgccttcg agcatgaccg ttccaccgta gcagcgagcc atcgataggc
    10441 tagatgcatt gggactggga tttacccaac tgcgtcggcc gagaggccaa ccaaatcccg
    10501 gtccagtcga cggctgccaa tcatccatgg tcacgacctc acaatccagg tccagccgga
    10561 gcgcgcgcac gaaccagcgc gcaagctcgc ccaaggtcag gccgtggcgc atcggcaacg
    10621 gcccggcgcc gacaaagctt tcccagccat cgcgcaagcg cagcccttct atcggccgtc
    10681 caataggatt gggccgatcg agaacccaga ccgtcttctt tcgttcggct gccgcctcga
    10741 gcatgtaacg caaggtggtc acgaacgtgt agatccggca accgagatcc tgcagatcga
    10801 tcaggaggat atcgaaacta tccatcattg ccacggtggg gcggcgcact tcgccgtaca
    10861 gactgaaaac gggaatgtca tggatcgggt cggtgaagtg cggcgattcg atcatgttgt
    10921 cctgcttatc accgcggagc ccgtgttgag gaccgaaagc ggcgtcgagt ttgacatcat
    10981 tacacgcggc gagagcgtcg agggagtgtt gaagatggcg cgtcactgaa gcgggatgcg
    11041 ctaacaatgc cgaccggcgg ccttttagcg ctttacgtag tgccggctct tctaacaacc
    11101 ggtcgatgcc gaatttcatc gcttgccagt ataagccgga tcacggattg cagctacatg
    11161 tggaggccat tccaaaacaa atgcgctggc atggtgaaag tcgggcctgc cgggaggatg
    11221 cttgagcgcc cagtaagacc tcaccccgtc ttgttcttcg atcactgctg acaaccccag
    11281 caacaagcgg ccattcactg gcaccgccgg caacgatgct tgctggatga gcgcatcgag
    11341 ttcgaaacaa tttgcagaag cgcgccgcgc aatctgcggg tctgaagcct gcggcagcgg
    11401 ggcgccttcg cgataacttt taaaaccata agccgcccac tggccggagg gagcgaagtt
    11461 gaactcgtga tatccaggcg catctttcac ggctaagaac gcttcgaagc aggtgtgctg
    11521 ccaaagcccg tctatcctgc tcggagcgga tctccccgga agacataacc ggctgatctg
    11581 tccctcgata aagtacgcga ttgcaatccc gccgttagga gcttgtgaca cgcgcgctcg
    11641 gatcgtcctc accgattggc tgggcgtttc gggatggcac gcgagcgcca cttcctgtgc
    11701 aattagagac atgatcctcg atttgaaccg gtcattgctc gtaaagccca tccaaccgat
    11761 ctatctctgc ttgacgaacg agcgaatgcc cattccaagc ttttgccgca acagtggacc
    11821 tgttggctgt catggcaagc aacgggttga tcgcgttgaa ctccattcac agagcgggta
    11881 cagatgacca tatggacaag gtccgaagtc tccatgacgc tatcgcgcag gacgttgccg
    11941 acggcgattc gatcgcgatg gggtgcgggc tggaatcgct gattcctttc gccgccagcc
    12001 atgagattat acggcagaga aagaaaaacc tcaccttgat tgcaccgata tccgacatgc
    12061 agttcgatca actgatcggc gctggttgcg taaaaaagat tatcgcctcc tgggtcggca
    12121 atgtcgccgc cggtctcggc cacaattacc ggcgcgcagc cgaatccggc ataccgcatc
    12181 cgatcgaaat cgaggagcac tcgaatttta ccatcggctt aggcttgcaa gccgcggcca
    12241 ccggcctgcc gtttatgcca accaaatcga tccgcggcag cgacatcagc cgctgcccgc
    12301 agtttgcctc ggtgaaatgc ccgttcaccg gcgatgaact cctagccgtc aaagcgatat
    12361 ctcccgatct ggcaattctt cacgtgcaaa gagccgatcg ccaaggcaat gcccacgcct
    12421 ggggaaactt cggtgtcatg agggaagctg ccttcgcggc caaaaaggtt attctcacct
    12481 gcgaagaaat cgtcgatcat gacgtgatct tgagcgatcc gaaccgaacg ctgattcccg
    12541 ggttcttggt ttccagtctc gttcatcagc cattcggctc gcacccgtcg ccgacgcaag
    12601 gctatgcgcg acgggacgac tctttttatt tcgaatacca caaagaaact cgttcccgcg
    12661 acggattcga aaattggctg caaacgtgga tcttcagtgt cgccaatcac ggtgaatttc
    12721 tcgagcggct cggcgccgac cgggtaaaac gactcgcgcc gcagaaaact ctaataaccg
    12781 agccggcaaa ttttagttac taaaacgaaa aaacaccacc taggaggaac tatgaaggca
    12841 gtaccctttt ttaagttaac ccaggaaggc gccgacatca tggtcgacgc ggccatcgaa
    12901 aaagccaaag aactcaagca gatcgtcagt atcgcggtcg tcgatagcgg cggtcacctg
    12961 ttttcattca agagaatgac caacggacgg acgcacaaca caatgatcgc gcttgccaag
    13021 gcccgctcgg ccgcgctgac tacggcggca accgggaaga ccaaccgcga gggcggcgag
    13081 attcccgatc accaagttct ggcccagact ttggctgtcg gtcccggctg gttcatcagc
    13141 atcgaaggag gaatcccgct cacgatcgaa ggccaatgca tcggagccgt cggtgtcagc
    13201 ggcgcccctt ccgccgtcga caaacaaatt tgccaggccg gcatcgatgc attcaatcag
    13261 gcaacctagt tttcatgcgg tggtctggtt tccggcaatt gggacgcaaa gctgaacacc
    13321 gaaaactagc tggctatgca ttacaccttg cgagagttaa tggttgccgc cgcctcccgg
    13381 gaaatccgcg atggcgataa agtctttgtc ggcatgcgcc tgccgctttt gggctttgcc
    13441 gtagccaagg agactcatgc gcccaacgcc ctgggcattt tcgagaatgg catcatcagg
    13501 gattggccgg cgctggagtc gattttaacc atgagcgatc caccgaatgt agcgcgcgcg
    13561 ttgagttgcg gcgggctcaa cgacgtgatg catcttttgc aaagtaatcg cgtcgactta
    13621 ggatttatcg gcggcgcgga agtcgatcgt ttcggcaacc tgaataccca ctgggttgaa
    13681 gagaacggga aacggattcg cttgccgggc agcggtggcg ctgcagatat tgcgacaatg
    13741 gccaagcggt gcatcatcat catgaatcac gaacggcgcc gtttccccgc cagagttcat
    13801 tacatcacct ctcccggatt cggcgatggc ggtgattggc gctcgcgcaa tggtctcatt
    13861 ggcagcggcc ccacccgggt gatcaccagc atgggcatat tctcattcga cgccagcaca
    13921 catgaaatga cgctcactgc aaaccatccg ggaacaaata tcgacgagat caagaatgaa
    13981 acgggttggc cgctcaggat agcaccggag ttgcaggaga ctgcggctcc cagcgacgct
    14041 gaactgtcgg cgatcagaaa atacgatccg aaagcgatct ggacgtcgta atgtcacgca
    14101 acggtcgtct aaacgtcgcc ttcccgaccc gaggactcca gcatcgccgt tctgggctgt
    14161 tactgccgat ggtcgcggta agataatgtt cgtatgaccg accgccgcat gaagcatgcc
    14221 ggcacgaggc ttacgggacc cgcgaccaac tcgcgagatc cctgcccgtt taacgatacg
    14281 tctgatccgg tcggtcagcc gtccaaacgc accgagttga tcacgactgg atccatcgga
    14341 acgtcttgat gcattccgct gttacgcgtc tttactcttt caatcttctg caccacatcc
    14401 attccgtcga tcacgcgagc gaagactgca taaccgaagg catccgcaga ggtgctccgg
    14461 tggtccaaga aatcgttatc tttgacgttg atgaaaaact gagcagtagc gctgtcgaca
    14521 acgttggttc gcgccattgc aaccgtgccg tttttgtttt tcaaaccgtt tgtcgcttcg
    14581 ttcttgatcg gtgacttggt gctcttctgt ttcatctgtg ggtccattcc gccaccctga
    14641 atcatgaaac cgggaatcac ccgatgaaaa atcaacccgt cgtaaaatcc gtcattgaca
    14701 taatccaaga agtttttcgt cgtgatcggc gccttgtccg catcaagctc aatccgtatg
    14761 tcgcccatgc tcgtcgatac cgtgaccgtc ggattctttt ctgccattac cgcctcctgc
    14821 ataagatgat gaatttctat cacctttgac ctcagtgcga aacggatatc ggctggaatt
    14881 gtcggcgacg gtgcgatatg ggtacgaaaa ctaacatgtg attcaaaact ggaaaggagt
    14941 ctttatgccc agcgatagaa agatggaagg gattttggtt ccgatgccgg tggcgttcaa
    15001 gaatgacggc gccatcgatc agagcgcgac cgatgctatt atcgatttct atcttgcatc
    15061 gggagtgcac ggcttttttc ctttaggcac tcacggtcaa ggcatggtta tggagatcga
    15121 cgagcgcaag aaggcggccg agcagatcgt taagcgggtt gccggaaaag tcccggtggt
    15181 catgcacatc ggcacggcca atacctattc ctccatcgaa ttggccaagc acgcggccgg
    15241 ccttggcgtg gacgctatcg gagtcgtgcc cccctactac tacccccatg acgactatga
    15301 ggtgtacgct cattacaagg cgattgccaa gtcggtcccg ggcgtgccgc tcttcatcta
    15361 cgacaacacc gaaactactc acgtcgagat cacaccgccg aaagcaaaca agatcatcga
    15421 agcgattgcc ccaaacaccc tggcgggcat caaagtgtcg ttcattccct acgacaaatt
    15481 attgggctac gtttttcaat tgccgaagtc ggtcggggtg ttccccggcg gaatcttctc
    15541 actgtatagc ggctactctg tcggagtgcg cggcgcgatt cacccgccgt ccactccgtt
    15601 tcccgaagcg tgcgttagaa tgtacgaggc cctgaaagcg ggcaaccatc aagaagcgca
    15661 aaagcaacat gatctcttgg ccgcagttct tcaagtcgtg ggacggtttc tccgcaccca
    15721 tgggcgcggc gtatttgccg agttgatgcg catgcgcggt ttgccggtgg aacgtttccc
    15781 gagatgggaa acccctccgt tcacggacaa ggaccgggcg gatttgaaag aaagcctgca
    15841 acaggccggc ttgaagcttt aactctaacg aggcgataga tgcatcgcag acaaatgcga
    15901 tcgtcttgtc tccgccgtta aggaattcgc gcttatgtga ggatgccgag gagatcctag
    15961 ttgcaacctt caagtcagct tcttgatttc ggattagcgg cgccatgcga ggctgctaca
    16021 gggtctcctc gcgcctcatc cttgctacga tgcttaggtt cggatacagt cgttacgcca
    16081 caatttcctt cagccgtact ggcgcgtaag aggtagaagg ccccaccaca ccctcatcgg
    16141 tggcatgtcc tttggctccg gatcgataga tcttagcggt tccgactcac tcctcttcca
    16201 atatccaaaa gctagcaata gctttaaagg agcattatga atcctctatt gatcatcgcc
    16261 gtccccttag cgttggtgat cctgggcatg atactgaaca gcacggggct ttcttacgat
    16321 caaccggcga gctcatccga gcaagatccg attaaaaaat tgaccgcgga aaaggatgct
    16381 tatcgtaaat ttttcgatct gcagagaagc cgatccctaa aacggcagca acggataagc
    16441 caatatagct ggcttgtgat ggctgcattc atcggcgcat tcatttggtt gtatacggat
    16501 acggtgaata agaccagcct gtcgagtcgc attgccggga tgcaaacctt ggcaacggaa
    16561 gaaggcaagc aaatggttct ctctgtgacg ttgagcgacg gcaataacgt taaatacctc
    16621 gtcaaacttc ctcaagccga aaaattggaa actgctccta aacaaggtat ggcggtagaa
    16681 aaggtctcgt cttgggagct agagagatta ggcaccgctc tgagcgtcgg cagtagtcct
    16741 ttgccggtag gcatggcatt gaaaatttcc cactaacgaa gtcagacaag cagattgaat
    16801 tggctcaacg ggctcgaggc gccgaacggc gccttgagtt catctttcac agagaatccg
    16861 tcgaagaacc cgagagagca tcagtcctaa caggctattt cagctggccg ctccgatcag
    16921 tcctagagct ctgacacaga ttcgaaacgg caaccttccg tgagacatac cttagtgagt
    16981 cttactcgac ctccagagca aaacatatat ttaacagcct tccagtcttg cgaggttaat
    17041 aaaccccgat caagaggcat catcactgga tattcgttgg gttcgagata gctttaagtt
    17101 tctgcaaaaa catttttggc ggctcctctc tatcgatcgc ccagtttgac tcggacgacc
    17161 ttacgtagat ttccgatctt tagtccccgg ttcagcgctt cgcggagagt ccacggaaat
    17221 agctttagaa ccgtaccggc tcgcatagac ttgggttaac ttggcgccga agaagaacat
    17281 aagtgatgaa tagtagatcc acaaaagcag cgtcaccact gacccggccg cgccataaat
    17341 ggatgtgccc gtgctgtgac ccagataaaa ggcgattaag aatttgccgg cggaaaatag
    17401 caaggacgtc atggcagctc ccatccagac atcccgccac gcaatccgta cgtcaggcac
    17461 gaatttatat atcgcggcga acagcagtgt gttgaacgca aacgagaatg cgagatcgaa
    17521 agcgtatgcc gtagcagcgt cgatggtcag aaagccgccg agtattcccg aaatcgctgc
    17581 caagactgcg ctgattatta aagagacgag taacaagaag ccgacgctta acaccattag
    17641 atatgagatc aaacggtcct gcaaaaaccc cctgacccct cgaccggttt tcggcacgac
    17701 tcgccaaatt gtattaagcg agtcttgcag ctgtcctact acacctcccg cacccaaaaa
    17761 caagaaaata atcgctatag cggaggcgaa aaaacctgag tcgggccttt
//
LOCUS       DASUVS010000297         3931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_994030, whole genome shotgun sequence.
ACCESSION   DASUVS010000297 DASUVS010000000
VERSION     DASUVS010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994030"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VF182_16120"
     CDS             complement(<1..276)
                     /locus_tag="VF182_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX6438665.1"
                     /translation="MSWFLHIDMDAFYASVEQAIDPSLKGKPVIVGGRDGKGVVTSAS
                     YEARKFGVRSAMPGFQANKLCPHGIFLPNRHRLYSEYSHKVFAILRQY"
     gene            complement(483..1913)
                     /locus_tag="VF182_16125"
     CDS             complement(483..1913)
                     /locus_tag="VF182_16125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6438666.1"
                     /translation="MQPADAKKTRWTLRLILLNVCIGQTVVGLDQRAITVALPTLTDT
                     FHTAFTTVQWVVLVYDLVLIGLIITMGRLGDLFGRRRFYSAGFLVFIAASALCGISQT
                     PGQMIFFRAIQAIGGSMIAANGRAIVSVALPREERGRALGLTSTAFHIGFLTGPSLGG
                     FLIDTIGWRWIFYINLPIALWGAYLAWRIIPETKAEGKISVDIPGAILLLVTNGLFIY
                     AIDLLPRLGWRHPTFLLILLLSLVAMTLLLFAESKAKTPILMLSMFRTRLFSAGILSL
                     FLITASLSAINFLLPFYLQNLLGYSPSQMGWIIIADSLIIMFMAPIAGTLSDRFGSRL
                     LCTIGCVVIVIAQFFVGSLGLESSLPRIMLPLALWGVGWALFNAPNQSATLGAVTQDK
                     IGAAAGMIATTARTGSAMGVALAATLFSTLLSAAGLSPAQTQSPQSWSTMPESVIGPF
                     NYTIHFINGLTLLAVFFSAVRGRRRD"
     gene            complement(2159..3592)
                     /locus_tag="VF182_16130"
     CDS             complement(2159..3592)
                     /locus_tag="VF182_16130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6438667.1"
                     /translation="MANSTSNETRRDPRLVLFLVCTGLFMVAINARAMIVSLPTLTEV
                     FQTTLSVIQWALLVYDLAVIGLVLTLSRLGDLFGRKWVYIIGFLLFTFGSALCGLVQN
                     PAQLIACRIVQGMGGAMIMAHGRAIASIVVPVSQRGKALGLTSMAYHVGYMTGPTLGG
                     FIIDTIGWRWIFYVNIPTGLASAYFAWRVLSDRAPREERVKVDYAGAILLLITNTCFV
                     YAMNQFPHLGFSHPLVISFAILSAAALFFFIRTELRVRTPILDLNLFRNRLFSAANLS
                     LLFITGTQSAIAVLMPFYLQNLMGFTPTQMGWIIIANSVVIIMVAPIAGGLSDRFGSR
                     LLCSIGATIIVIGQFLIASWDQHSTVFQMIFPQALTGLGWAVFNSPNQSAILGTVPRN
                     KVGAASGMTVTTARIGAVIGIALSGLLLTNGLYRGGMLAEQIESPQSWGASPEIFLDA
                     FSHAIHVINSFAILAIVFSAIRGGSKD"
BASE COUNT         1020 a         1040 c         1107 g          764 t
ORIGIN      
        1 gtactgccgc aggatggcaa ataccttgtg ggaatattcc gaatacagcc gatggcggtt
       61 aggaagaaat atcccgtgcg ggcacagctt attagcttga aaccccggca tcgccgaacg
      121 gacgccgaat tttcgcgctt cgtaggaggc cgacgtaacc acgcccttac catcccgtcc
      181 gccgacgatg accggcttac ctttcaagct cggatcgatc gcctgctcca cggacgcata
      241 gaatgcatcc atgtcgatgt ggagaaacca actcatggtg tccgagtata gaaactatcc
      301 ggctgcaacg gaaacccaaa atgagtctgt cgtcaactat ttgacgcgcg ccgagcggcg
      361 ttattgatcg ccttgaagtt taccggagca aaatccatga ggcagcgatc gatcggatga
      421 agagccaccg cactagccat cgacttttga acagagaaaa actgtactcc cattataatc
      481 gattagtcgc gccggcggcc tctaaccgcc gaaaagaaca ccgccagcaa agtcagtccg
      541 ttgataaaat gaatcgtgta gttgaacggg ccaatcaccg attcgggcat cgtgctccag
      601 ctttgcggcg actgcgtctg tgccgggctt aaacctgccg ccgataacag ggtgctgaaa
      661 agagtcgcgg ccagcgccac acccatggcg ctgccggtgc gcgccgtcgt ggcgatcatg
      721 cccgccgcgg cgccgatctt gtcctgcgtg actgctccca gggtcgcgct ctgattgggc
      781 gcgttgaata acgcccagcc gactccccag agcgccaggg gaagcatgat tcgcggtagc
      841 gatgactcta ggccgagtga gccgacaaaa aattgcgcga tgacgatgac gacgcaaccg
      901 atagtgcaga gcagccggga gccgaagcgg tccgacaagg ttccggcaat cggcgccatg
      961 aacatgatga tcagtgaatc agcgatgatg atccagccca tctgagaggg tgaatagccc
     1021 aacaaattct gcaaatagaa gggcaggagg aaattgatcg ccgacagact cgcggtgatc
     1081 aaaaagagac tcaaaatgcc tgcgctaaac aggcgcgtgc ggaacatcga tagcattagg
     1141 attggcgttt tcgccttgct ttcggcaaat aggagaagcg tcattgcaac cagcgagagc
     1201 agcaatatca gcaggaacgt cggatgacgc cagccaagcc gcggcagcag atcgatggcg
     1261 tagataaaca aaccgttggt tacaagcagc agtattgcgc ccggaatgtc caccgatatt
     1321 tttccttcgg ctttggtttc cgggataatt ctccaggcca ggtaggcgcc ccaaagagcg
     1381 atgggcaggt tgatataaaa aatccaacgc cagccgatcg tatcgatgag aaagccacct
     1441 aacgacggcc cggtgagaaa acctatatga aacgctgtcg atgtaagccc caaagcgcgg
     1501 ccccgttcct cacgcgggag cgcgactgaa acgatggctc tgccgttagc ggcaatcatc
     1561 gatccgccaa tcgcctgaat agctctaaag aagatcatct ggcctggagt ttgagagatg
     1621 ccgcaaagcg ccgaagcggc aataaacacc agaaaaccgg cgctgtaaaa gcgccggcgg
     1681 ccgaacaaat ctcccaatcg gcccatggtg atgatcagtc cgatgagaac cagatcatag
     1741 accagaacta cccattgaac ggtggtgaat gcggtgtgaa aagtgtcggt caaggttggc
     1801 agcgcaactg tgatcgcgcg ctggtcgaga ccgacaacgg tttgtccaat gcagacattt
     1861 aaaaggatca gccgcagcgt ccatcgggtt ttttttgcat cggcgggttg cattatttca
     1921 gctgcgttca attaacttct tttccgaaga ccgtcattcc cgcatgcatt tagcgggaat
     1981 ccaggcgaga ccggaactgg acccccgata aaaacattcg ggggtgacgc gtttcggata
     2041 gcgtcctttc tgataccccg cagctgctgc ggggtagttc agtccctggc atgagcctcc
     2101 gggaggtcac gacagcagtg gtcttaacca gtcgagcttc ggcagaacca cgaatgtatc
     2161 agtctttgct gccgccgcga atagcggaga agacgatcgc cagaatcgca aaactattaa
     2221 tgacgtgaat ggcgtgacta aaggcgtcga gaaatatttc aggcgatgct ccccagcttt
     2281 gcggcgattc gatctgttcg gcaagcatgc cgcctcggta aagtccgttg gtaagcaaaa
     2341 gcccggagag ggcgatgccg attaccgcgc caatgcgcgc cgttgtcacc gtcatgccgg
     2401 atgcggcgcc aaccttattc cgggggacag tccccaagat ggcgctttga ttcggcgagt
     2461 tgaaaaccgc ccaacccaag ccggtcaagg cttggggaaa aatcatctga aacaccgtcg
     2521 agtgctggtc ccaagaagcg atcaagaatt ggccgataac gatgatggtt gcgccgatgc
     2581 tgcaaagcag ccgtgaacca aaacgatccg aaagaccgcc ggcgatcggc gcaaccatga
     2641 tgatgaccac cgaattagcg atgatgatcc agcccatttg agtcggcgtg aagcccatca
     2701 ggttttgcaa atagaaaggc atcaaaacag ctatcgccga ttgggttccg gtaataaaaa
     2761 gcaggcttaa gttggccgcg ctgaatagtc gattgcggaa aagatttaga tcgagaatcg
     2821 gagttcttac gcgcagctcg gttcgaataa agaagaagag cgccgccgcc gaaaggatcg
     2881 cgaaggatat taccagagga tggctaaagc cgagatgcgg aaactgattc atcgcataaa
     2941 cgaaacaggt attcgtgatc aatagcagga tcgcgccggc ataatcgact ttcacccgtt
     3001 cttccctcgg cgcgcgatcg ctgaggaccc tccacgcaaa gtatgcgctt gccagtccgg
     3061 ttgggatatt gacgtaaaaa atccaacgcc agccaatcgt atcgataatg aagccgccta
     3121 aggtcgggcc ggtcatgtag ccgacgtgat aggccatgga ggttaatccc agcgctttgc
     3181 ctctctgtga aaccggcaca acgatcgagg cgatggcgcg gccgtgagcc atgatcatag
     3241 cgccgcccat tccctggacg atgcggcagg cgatcaattg tgccggattt tgcaccaacc
     3301 cgcacagagc cgacccgaag gtaaagagca ggaaaccgat gatgtaaacc catttgcgac
     3361 cgaagagatc tcccaggcgg ctgagagtga gcacgaggcc gatgacggcc aggtcgtata
     3421 ccagaagagc ccactggata acggacagag tggtctggaa aacttcagtc aaggtcggga
     3481 gggacacgat catggcgcgc gcgttgatcg cgaccataaa cagtccggta cagaccagaa
     3541 acagcacgag acgtggatcc cgccgggttt cattgctggt tgaattggcc atgcattacc
     3601 cgcttgctga aatctgaatt agagtttgcc gaaaaacagc acacttgaat tccgctgatc
     3661 tgagacctac gaggaggagt atcccggaaa tagggcgcgc gcgccaacaa gcgcgaagct
     3721 tttcggcaga gctggaagta aaaagagagg cagcccgact ggactgcctc tgttatgaga
     3781 aagatcaacg aatgcgctaa gaagatctgc gcgataggct agctttgagt tcctgccgaa
     3841 gttctgcttt gcgatgctgc cgttgatcga tctttttgct cttgaaaact ttggttggag
     3901 gaggaacttc tttgcgaacc tgtgccagcc c
//
LOCUS       DASUVS010000298        14697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1432578, whole genome shotgun sequence.
ACCESSION   DASUVS010000298 DASUVS010000000
VERSION     DASUVS010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14697
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432578"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VF182_16135"
     CDS             <1..624
                     /locus_tag="VF182_16135"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6438668.1"
                     /translation="NYARQIMPRYMMAPDGARMVILGMGRIGQDIARKAKAFDMEVIC
                     VTRAAAPAVPEIDQVVPREKVDEVLPTADVVMNAMPLDSSTQGFVSTERIALMKETAV
                     FVNISRGKVVDEAALARALAEGRIMGAGLDAFAREPLPADSPLWDLPNVLMTPHVGGN
                     GGRELWRRMSELVRDNTRRYLLGAPLKHVVRAPDGTLQGFENLQSTP"
     gene            complement(647..1057)
                     /locus_tag="VF182_16140"
     CDS             complement(647..1057)
                     /locus_tag="VF182_16140"
                     /inference="COORDINATES: protein motif:HMM:NF023520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3574 domain-containing protein"
                     /protein_id="HEX6438669.1"
                     /translation="MQLFHLAIIATVALTIDACSSTYILRCKEGYQLAVHDSLYFGTA
                     KRDGVVSAEDWARFLESTVTPRFPQGLTSTRASGQWQGPDGEIVREDSHVLQLLHPDD
                     EPTEKAIRELIDIYKRQFKQDAVLRTISHTCASF"
     gene            1161..1823
                     /locus_tag="VF182_16145"
     CDS             1161..1823
                     /locus_tag="VF182_16145"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6438670.1"
                     /translation="MVDQDYAAVKDDFITALRIIYREGLSDAFAHLSLRSPEGNEMMF
                     MPRKSPALVTSDELFFVDFERTVPQSSLHQAIYKTRPDVKAVFHFHSPAVILLSVVGQ
                     TIRPMHNYSAIFYEGVPLYTGTGQVESPSRASEMAKLLGNAKALMLRGHGAIVVGQSI
                     REVCMLALFLEESGRLQAEATRLGTPMFMERDEAERIAKRTFKPASIERAWNHFAAKH
                     AD"
     gene            complement(2078..2764)
                     /locus_tag="VF182_16150"
     CDS             complement(2078..2764)
                     /locus_tag="VF182_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEX6438671.1"
                     /translation="MRIAEIFYSIQGEGRLSGAPSLFIRTSGCNLRCRWCDTPYTSWN
                     PEGDEMSLEEIFHAISSYPLIHVVLTGGEPLLSHEIEDLTAKLKAAGAHITIETAATI
                     FKPVSCDLVSMSPKLSNSTPWQKQNGRFAAMHEQHRLNFKVVQQFIDGYDYQLKFVVD
                     QEQDFAEVQQVLDALQNVDTTRVLIMAQARNRLQLHQKSRWIVELCKKFGYGYSPRLH
                     IELYGNRRGV"
     gene            2935..3177
                     /locus_tag="VF182_16155"
     CDS             2935..3177
                     /locus_tag="VF182_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438672.1"
                     /translation="MEIDKEALLAEERRIRRLRRAMDIAVALLWQVDLTLAEAQDVVE
                     HAKKTALQLFPDKEETFDLIYGSRFRRVLVEKYRLQ"
     gene            3186..8867
                     /gene="uvrA"
                     /locus_tag="VF182_16160"
     CDS             3186..8867
                     /gene="uvrA"
                     /locus_tag="VF182_16160"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX6438673.1"
                     /translation="MSKHSIRIRGAKQNNLNNLDLDIPLNALTVVTGVSGSGKSTLAF
                     DILYAEGQRRYVESFSAYARQFLDRMDKPDVESIEGIPPTIAIDQSRPVRTSRSTVGT
                     MTELHDHFKLLFAKIGILHCRGCGRIVERDSSLSIVNKLSSLPAGSPMVLTFSLPRSP
                     VDWNEVRSGLQQAGFHRLLVDSAIEDLEAVDTAPSGKLLEVVVDRFAFRLENKKRICD
                     SLEQAFHYGKGKLNVFLPAEDWRREPFSNHLHCPHCDISYPEAVPNLFSFNSPLGACE
                     TCRGFGRVIDIDLDLVIPNPEKSLSDGAIKPWNSKPRRLRRLLDFCERKRIPVKKPYG
                     ELTDQHKALIVGGDGAFKGIRGWFRRLEQKSYRMHVRVLLSRYRCYLLCPDCQGSRVK
                     PGALNYLIDGKNIAQVSAMSVGAAHRFFNQLKPAGSLDQVAALILEEIRRRLSYLDSV
                     GLEYLTLDRQSRTLSGGELERVDLTTAIGSSLVNTLYVLDEPSIGLHPRDSRRLVEIL
                     HRLKANHNTVVVVEHDPEIIKECDHIIDMGPKAGQQGGNVMFAGSYEGLLKDDKSLTA
                     AYLSRRKTIPLPAGTRKSLSRRSIKISGAQANNLKNIDVEIPLGLMVCLTGVSGSGKS
                     SLVDEVLYRNLKKLKESPSAKVTQCAAITGADRISEVVLVDQSPVGTTPRSNPATYMK
                     AFDGIRKLFAATDLSRLRGYAPSTFSFNVEGGRCESCRGEGFEKVEMQFLSDVYTSCP
                     ECHGSRFRQEVLEVNYRGKNIRQILDLTIAKALEFFEDTGEIKNALTPLQAVGLEYVC
                     LGQPLTTLSGGESQRLKLAWHMAKARKAGTLFIFDEPTTGLHFHDIERLLQAFNELLE
                     QGHSIVIIEHNLEVIKCADHIIDLGPEGGDKGGEIVAVGTPEEIAAVEASHTGRYLRP
                     YLKRSKVSPYTPTIVNRSPVQNLNGSTTEDNAIAIVGAKEHNLKNISIDIPRDRFIVF
                     TGLSGSGKSSLAFDIVYAEGQRRYIDSLSAYARQFLEVMARPNVDFISGIPPTVAIEQ
                     RLSQGGNKSTVATVTEIYHYLRLLYSKIGKQHCVQCGRRIQSLTRSQILDRIGRSYRG
                     KEVMILSPIVRGRKGFHKEVIAGARKLGYRRARIDGELMDLRAPELSGGLERFQEHNI
                     DIVIGKTKVGGREIEALVDQGLRLGNGVIHLVSERGQQVFNQRLFCLECGVGYEPLDP
                     RFFSFNSRQGACQECSGLGFSWDFDPELIFIDPGQPLNHAIAGISVGSSAGEADLQRA
                     LQKFLHDLKDKQRLDIEQPLAKLPKKLQDEILYGGGGRSGCVGLVPFLKELQDDAADE
                     LHSVLDELMTETPCPACVGRRLNPRAQAVKLDGKAIWQVTSFSVDEANEYFAKLNLSF
                     SDNGNSERDRAVADKIVKEIQQRLTFLSEVGLPYLTLDRRADTLSGGEAQRIRLAAQL
                     GSNLRGVCYILDEPTIGLHPRDNDMLLRTLRRLERLGNSVLVVEHDEATIRSADLIVD
                     LGPGAGVHGGSVVSIGSPDEIRNNPDSPTGAYLRSVKRRLGPQRDLDKADWLTIRGAK
                     AHNLKNIDVKIPLGMWSCITGISGSGKSTLVREVLYKGVKLLLGQFAGRPGAHKEIAG
                     WKAIERIVEVDQTPIGKTPRSVPASYVGLLDEIRKLYALSPEARLRGYTPSRFSFNVK
                     GGRCEACAGQGKIRKEMSFLPDVFVDCDDCRGERFNEETLNIRFNGRNIADVFHMTVE
                     EAVPFFQAFPKIARPLKVLDDIGMGYVTLGQASNTLSGGEAQRIKLAYELGKDSRGKT
                     LYLLDEPTTGLHFADVDKLIRILHRLVDRGNTVITIEHNLDIVKDADYLIDLGPEGGE
                     QGGRLVAAGTPLQVSKDGRRSYTARFLREYLNRGSESGETVSPPILEQVIA"
     gene            8962..9261
                     /locus_tag="VF182_16165"
     CDS             8962..9261
                     /locus_tag="VF182_16165"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX6438674.1"
                     /translation="MHTTCPHCGLVFEREQGYFIGAIYINYAATVLIAVPGFFIMSIF
                     TNLTIDQQLWIWISFAVIFPLLFFHHSRSLWLVLDHIFNPPEKFYSVPPKKSQRS"
     gene            complement(9427..10287)
                     /locus_tag="VF182_16170"
     CDS             complement(9427..10287)
                     /locus_tag="VF182_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6438675.1"
                     /translation="MTFGALILILAAAFIHASWNLLNKRASGHVTFTWLVAVLSAIFY
                     TPLALGVIFVTHLQIGVVELALMAGSAALHSAYFVLLNQGYRVGDLSLVYPLARGTGP
                     LLSTISAIFLLGERPSALAVAGALLIICGVVVITGSPKSLRGSEVKTAVLYAAVTGLF
                     IAAYTLWDKQAVSRFAVPPLILDWGSNLGRTVLLTSLPARYPRETRVEWREHRFEAIG
                     VAVLAPLSYILVLTAMQFTPVSYVAPAREISILIGTAMGTRLLAEGEGRRRLAAASAM
                     VLGVAALAIG"
     gene            11089..>14697
                     /locus_tag="VF182_16175"
     CDS             11089..>14697
                     /locus_tag="VF182_16175"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6438676.1"
                     /translation="MDTALPNRSKYVEGFRYQEDFRIQLLLLALLIGIAYYLGALLSF
                     TLRVPSTRSSILWVPNAILLAAFVVTPVKRWWLWILAAVPAHLLAQSRDAAPVLVLLC
                     PFLANVAQAALAAVSLRLLTDVPHRLESLRSMGIFILVAVIAAPALASFTCAWLFVLA
                     GWETDYWLVGQARWLNNVVTGLTAAPLCLAIAGGDLYKLRCLQVRRYGEFAVVILGLV
                     LILSLTSAWHAAETIDFPIRLYAPLPFLLWAAVRFGPPGLAIALLAVAYDSISDSIFG
                     QAPSAASSPAINVLALEASLGVLSLPLMLLAALMMERQHKDEALRESQERLARTEKFS
                     LVMVTHTDLEGRWLKVPPTLCELLGYNEQELLGRRFDELTHPDDVEINVTRRLQLLRN
                     EIKSFDLEKRYLRKNGMVVWVYINVSVVTDRHGSPVHFLSYIRDISRRKEAEQGLRES
                     EERLQLALEAGGMGVWDWDTCNNTLDWSSEQFTIMGFKPFSIRPTYQSWTERVHPDDR
                     AHVSAAIERAIAEQSHYRSEYRIVLPDGSHRWLISRGEAVYKTGGEYVRLAGVTVDIT
                     DRKRAESRLNVQYQIARVLSNSASLTDAAPALIQRICNSFDWHCGEIWQPSPAADVLT
                     YLEGWHSPSMKLTPFVSESRRYTCSPGMGLTGRVWKSGTPAWIIDIADDDNFPRASMA
                     EKSGLKSAFSFPITVGDRTLAVMAFFSTDVRQPDEELLQTMDSIGRQIGQFIERKNAE
                     QVTFQRDQLLHTMFESLSSHVVVLDCDGTITYASKLAEQFTTSQLLNNLSVGINYLDV
                     CRRAASLEDGNAKEALCGIEDVLTGQLSSFSMEYRCDANQCARWFAMQVDPMPRDQGG
                     VVISHADITERMQVDEALRDALNEVRKLKEQLELENTYLREEVSEVHRFGEIGGRSES
                     ISKALRHAELVAPTDAAVLIMGETGTGKELLARAVHARSKRSDRPLVKVNCAGLPATL
                     IETELFGHEKGAFTGAAARRIGRFELADGATIFLDEVGELPLELQAKLLRVLQEGEFE
                     RLGSSKTMTVNTRVIAATNRDLGLAVKAGTFRDDLYYRLNVYPIKLPPLRDRKEDIPE
                     LVQTFLREASRRLGRPLDGVSQRVMDAFMKYDWPGNVRELQNVIERMTVTAKGAQLSL
                     PSDWLTGGSLTDMPFDLFKSQEAHCAVSSEEETTVENVERAHILRILEQRHWRIEGP"
BASE COUNT         3255 a         4027 c         4182 g         3233 t
ORIGIN      
        1 aattatgcgc ggcagatcat gccgcgttac atgatggcgc ccgacggcgc gcgcatggtg
       61 atcctcggca tgggccgcat cggccaggac attgcgcgca aggccaaagc gttcgacatg
      121 gaagtgatct gcgtcacccg cgcggcggct cccgccgtgc ctgaaataga ccaggtggtg
      181 ccccgagaaa aggtcgacga ggtgcttccc accgccgacg tagtgatgaa cgcgatgccg
      241 ctcgattcca gcacccaggg atttgtgagc actgaaagaa tcgctctaat gaaagagacg
      301 gcagtgttcg tgaacatctc ccgcggcaag gtggtggacg aggcggcgct ggcccgcgcg
      361 ctggccgagg gccgcatcat gggcgccgga ctcgatgctt tcgctcgaga accgttgccg
      421 gcggacagcc cgctttggga tttgccgaac gtgctgatga cgccgcatgt cggcggcaac
      481 ggcgggcgcg aattgtggcg gcgaatgagc gagctagtgc gcgacaacac gcgccgctat
      541 cttttgggcg cgccgctcaa acacgtagtc agagcaccgg acgggacgct ccagggcttt
      601 gaaaacttgc agtcgacccc gtaggggggc aagtgaaccg atctcactag aacgaagcgc
      661 acgtatgaga aattgtgcgc agtaccgcgt cctgtttgaa ttggcgcttg tagatgtcga
      721 tcagttcccg aatggctttt tcggttggtt cgtcgtcggg atgaagcaat tgcagcacgt
      781 gggaatcctc ccgaacgatt tcgccgtcgg ggccttgcca ctgtcccgag gcgcgtgtgg
      841 aagtcaggcc ttgaggaaat cggggagtga ctgtgctttc cagaaatcgt gcccaatcct
      901 ccgccgagac aacgccatcg cgtttggcgg tgccgaagta aagcgaatcg tgaacggcta
      961 attggtaacc ttccttgcag cgtaaaatgt acgttgacga gcacgcatcg attgtgagcg
     1021 cgacggtagc tatgatcgct agatgaaaaa gctgcatgag gcaaactctg ctagcgctga
     1081 tgggtttttg gcgagtatag tccgctgaag cgaagaagca aatttttcta gccaacagtg
     1141 tggcggaaag gataacgaga atggtcgatc aagactacgc tgccgtcaaa gacgatttca
     1201 tcacggcgct gcggatcatt tatcgcgagg ggctgagcga cgccttcgcc catctcagcc
     1261 tgcgcagccc cgaaggcaac gagatgatgt tcatgccgcg aaagagtccg gctttggtca
     1321 cgagcgacga gttgtttttt gtcgacttcg aaaggaccgt gcctcagtcc tccctacatc
     1381 aggcgattta taaaacgcgt cccgacgtca aagcggtctt tcatttccat tcgccggcgg
     1441 tgattctgtt gagcgttgtc gggcaaacca ttcgaccgat gcacaactac agcgcgatct
     1501 tttacgaagg ggtgccgctc tatacgggca cgggccaagt cgaatcaccg tcgcgggcga
     1561 gcgagatggc caagttgctg ggcaacgcca aggcgctgat gctgcgtggg catggtgcga
     1621 tcgtcgtcgg tcaatcgatt cgtgaagtct gcatgctggc gcttttcctc gaggagtcgg
     1681 ggcggctgca agccgaagct acgagactcg gcacgccgat gttcatggag cgcgacgaag
     1741 ccgagaggat cgccaagcgc actttcaaac ctgcatccat cgagcgggcg tggaatcact
     1801 ttgccgcgaa gcacgccgac tgattttgct tttcatcctc tagcccggat tattcacggt
     1861 gaccgcgtcg cagcgcgagc cctccggaat gaagacaaat gggagtgtgc cttggagcgg
     1921 cttgcataac ccagttgtag agcggatgcc tatcctcgtg atcgtgcccg tgcaaacgtg
     1981 atcgcgattc ccgaacgaac acgaacacgt accacgaaca cgagtttagc gaggtaattg
     2041 agcggcaacg aagcatatgg gtcttttcag cagcctgcta aaccccgcgc cggttgccgt
     2101 acagttcaat gtgcaggcgc ggtgagtagc cgtagccgaa ttttttgcac agctcgacga
     2161 tccagcggct cttctgatgt agttgcagtc ggtttctagc ctgcgccatt atcaggacac
     2221 gagtggtgtc gacgttctgg agcgcgtcga gaacctgttg cacttcggca aagtcctgct
     2281 cctggtcgac gacgaattta agctgataat catagccatc gataaattgc tgcacgactt
     2341 tgaaattgag ccggtgctgt tcgtgcatcg ccgcaaatcg gccgttttgt ttctgccacg
     2401 gcgtcgagtt cgacagtttg ggactcatag agaccagatc gcaactcaca ggtttgaaga
     2461 tcgtcgccgc ggtttcgatg gtgatgtggg ctccggctgc tttcagtttt gctgtcagat
     2521 cctctatttc atgggacagt aaaggctctc ccccggtgag caccacatga atcagtggat
     2581 agctgctgat ggcgtgaaaa atctcttcca ggctcatctc gtctccctcc ggattccagg
     2641 aggtgtaggg agtgtcgcac cagcggcaac gcagattgca gcccgacgtg cgtatgaaaa
     2701 gcgacggtgc tccgctcagc cgcccttctc cctgaatcga gtagaaaatc tccgcgatgc
     2761 gcatagagcc accctagcaa aggcaacagc gtggcggaag ttaacggtta accagcgcaa
     2821 ccaacgcttg acgcggtcac tttcttgact caccatcgcg gtatgttaag tgtattaggt
     2881 tcgggcgctt gaagattgct ctcaactgta agttcgctcg atcctctccc ggcaatggaa
     2941 atagataaag aagcgctgct ggctgaagaa cgtagaattc gcaggctgcg ccgggcgatg
     3001 gatatcgctg tggcattgct gtggcaggtc gatttgaccc tggccgaagc ccaagacgtg
     3061 gttgagcacg ccaaaaaaac cgcactgcag ctctttccag ataaagaaga gactttcgat
     3121 ttgatctatg gctcgcgctt tcggcgggtc ctggtggaga agtaccgcct tcagtaattc
     3181 acaatatgag caaacattcc atacggatcc gaggcgcaaa gcagaacaat ctcaataacc
     3241 tcgatttaga tattcccctc aacgccttga cggtagtcac cggcgtcagc ggttcaggaa
     3301 agtcgaccct ggccttcgat atactttatg ccgaaggtca acgccgctac gtcgagagtt
     3361 tttccgctta tgcgcgccaa tttctcgatc gtatggacaa acccgatgtc gagagcatcg
     3421 agggcattcc gccgacaata gcgatcgatc agagccgtcc cgttcgaaca tcgcgctcga
     3481 cggtcggcac catgaccgag ttgcacgacc atttcaaact gctatttgcc aagatcggca
     3541 ttttacattg ccgcggctgc ggacggatcg tcgaacgcga ctcttccttg tcgatcgtga
     3601 acaagctcag cagccttccg gcaggctcgc ctatggtgct gactttttca ttgccgcgtt
     3661 ctccggtgga ctggaacgag gtgcgcagcg ggctgcaaca agccggtttt catcggctgc
     3721 tggtagatag cgcgatcgag gatttagaag ctgtcgatac cgcaccttcc ggcaaactgc
     3781 tcgaggtcgt ggtcgaccgg ttcgcattcc ggttagagaa caagaagcgc atttgcgact
     3841 ccctcgaaca agcctttcat tacggcaagg gaaaactaaa tgtgttcctg cccgccgaag
     3901 attggcggcg cgagccgttc agcaatcatc tgcattgtcc gcattgcgat atctcctacc
     3961 ctgaagcggt gccgaactta ttttccttca acagcccgtt gggagcctgc gaaacctgcc
     4021 gcggttttgg gcgggtgatc gatatcgatc tcgatctggt gattccgaac ccggagaaat
     4081 cgctcagcga cggcgctatc aaaccatgga atagcaaacc ccgccgcctg cgccgcttgt
     4141 tggatttctg tgagcgcaaa cgcattccgg taaaaaaacc ttacggtgag ctcaccgatc
     4201 agcacaaggc gttgattgtt ggcggcgacg gcgcattcaa agggattcgc ggctggtttc
     4261 gccggttgga gcaaaaaagc tatcggatgc acgtgcgcgt tctgctgtcg cgctaccgct
     4321 gctatcttct ctgtcccgat tgtcagggca gccgggtcaa acccggcgcg ctcaattatc
     4381 tgatcgatgg gaaaaatatc gcccaggtaa gcgcgatgag cgtcggcgca gcgcatcgtt
     4441 ttttcaatca gctcaagccg gcaggttcgt tggatcaggt cgccgcgctg atcctggaag
     4501 aaatccgccg gcgcctaagt tatctggaca gtgtcggtct ggaatatctg actctcgatc
     4561 gccaatcgcg cacgctctcg ggcggggagc tcgagcgcgt cgacctaacc actgcgatcg
     4621 gctcatctct ggtcaatacg ctttatgttc tcgacgagcc atccatcggg ttacacccgc
     4681 gcgatagcag gcgcttggta gaaattcttc accggctcaa agccaatcac aataccgtcg
     4741 tggtggtcga gcatgatccc gaaatcatca aggagtgcga ccacatcatc gatatgggcc
     4801 cgaaagccgg gcagcaaggc ggcaacgtta tgtttgccgg ctcctatgag gggttgctga
     4861 aagacgataa atcgctcacg gcggcttatc tgagccggcg caaaaccatt cctctgccgg
     4921 cgggtacacg aaaatccctc tcacgccgct cgatcaagat cagcggcgcc caagcgaaca
     4981 atctaaaaaa catcgatgtc gagattccgc tcggactgat ggtctgtctg accggtgttt
     5041 ccggctcggg aaagtcgagc ctggtcgacg aggttctcta tcggaatctg aagaagctca
     5101 aagaatcgcc gtctgccaag gtgacccagt gtgcggccat cactggcgcc gataggatct
     5161 ccgaagtcgt actcgtggat caatctccgg ttggcaccac gccccgttcc aacccggcga
     5221 cttacatgaa agccttcgac ggcatccgca agctgttcgc cgctaccgat ttgtcgcgat
     5281 tgcgcggcta cgcgccctcg accttctcgt tcaacgttga aggcggccgt tgcgaaagct
     5341 gtcgtggcga agggtttgag aaagtcgaga tgcagtttct ctccgatgtt tacacttcct
     5401 gccccgaatg ccacggcagc cgctttcgac aggaggtttt ggaagtcaat tatcgcggta
     5461 aaaacatccg gcagattctc gatctcacga tcgccaaggc tttggaattc tttgaagaca
     5521 ccggtgagat caaaaatgct ttaactccgt tacaggcggt gggccttgaa tacgtgtgcc
     5581 tgggacaacc gctgacgacg ctctccggcg gcgagtcgca acgattgaag ctggcatggc
     5641 acatggccaa ggcgaggaaa gcgggcacct tgtttatctt cgacgagccc accaccgggt
     5701 tgcacttcca tgatatcgaa aggctgttgc aggccttcaa cgagctcctc gaacagggcc
     5761 attctatcgt catcatcgag cacaacctgg aggtgatcaa gtgcgccgat cacatcatcg
     5821 atctcgggcc tgagggcggc gacaaaggcg gagagatcgt cgccgttggc acgccggaag
     5881 aaatcgccgc cgtcgaagcc tcgcataccg gccgttacct gcgtccttat ctgaagcgca
     5941 gcaaggtttc accgtatacg ccgacgattg tgaatcgttc gcccgttcaa aacttgaacg
     6001 gttcaacgac cgaggacaat gccatcgcga tagttggggc gaaggaacac aatctcaaga
     6061 acatcagtat cgacattccc cgcgatcggt ttatcgtctt taccggcctg agcggctcgg
     6121 gaaaatcttc gctggcgttt gatatcgtct atgccgaagg ccagcgtcgt tacatcgaca
     6181 gcctgtccgc ttatgcgcgg cagtttctgg aagtgatggc gcgtcccaac gtcgatttta
     6241 tctccggcat accgcccacc gtcgccatcg aacagagact gagccaggga ggcaacaagt
     6301 ccacggtcgc aaccgtcacg gagatctatc actatttgcg gctgctctat tcaaaaatcg
     6361 gcaagcagca ctgcgtgcaa tgcggccggc gtattcagtc gttaacccgc agccagattc
     6421 tcgatcgcat cggccgctcc tatcgcggca aggaagtcat gatcctgagc ccgatcgtgc
     6481 gcgggcgcaa aggttttcac aaagaggtga tcgcgggcgc cagaaagctg ggctatcgca
     6541 gggcgcgcat cgacggcgag ctgatggatc tgcgagcgcc ggagctgagt ggcggcctcg
     6601 agcgctttca ggaacacaac atcgatatcg tcatcggcaa gacgaaagtc ggcgggcggg
     6661 aaatcgaggc actggtcgat cagggactgc gtctgggcaa tggcgtcatc catctggtct
     6721 ccgagcgcgg ccagcaggtt ttcaaccaga gattattttg ccttgaatgc ggcgtcggct
     6781 atgagcccct ggacccgcgg tttttctcgt tcaacagccg tcagggagct tgtcaggagt
     6841 gcagcgggct cggctttagt tgggatttcg atcccgaatt gatttttata gatccgggcc
     6901 aaccactaaa ccacgcgatt gccggcatct ctgtcggatc gtcggccggt gaggccgacc
     6961 tgcaaagggc attgcagaaa tttttgcatg acctaaagga caaacaacgg ctggacatcg
     7021 agcagccgtt agccaaactg ccgaagaaat tgcaagatga aattctttac ggtggcggcg
     7081 gccggtcggg ttgcgtcggc ttggtgccct ttctcaaaga actgcaagac gacgccgccg
     7141 atgaattgca cagtgtgctc gatgaactga tgaccgagac gccgtgtccc gcttgcgtgg
     7201 gccgccggct taatccacgg gcgcaagcgg tgaagctcga tggcaaagcg atctggcagg
     7261 tcacgtcgtt ctccgtggat gaggccaacg agtattttgc caagctcaac ctgtcgttct
     7321 ccgataatgg taacagcgag cgcgatcgcg ccgtggccga taagattgtc aaagagatcc
     7381 agcaacgact gacttttcta tccgaagtcg gcttgcccta tctaaccctt gatcgtcgcg
     7441 ccgacaccct ttcgggtggg gaagctcagc gcatccgttt ggcggcgcaa ctcgggtcga
     7501 acctgcgcgg cgtctgttac attctggacg agcccacgat cggtcttcat ccgcgcgaca
     7561 acgacatgct tttacgcacg ctcaggcgcc tcgagcggct cggtaacagc gtgctggtgg
     7621 tcgagcatga tgaagcgacg attcggtcgg ccgatctgat tgtcgatttg ggaccgggcg
     7681 ccggcgtcca cgggggcagc gttgtcagca tcggatcgcc ggacgaaatc agaaacaatc
     7741 cggactctcc caccggagcc tatcttagat cggtcaagag gcgactcggc ccgcaacgcg
     7801 acctcgacaa ggccgactgg ctcacgattc gcggcgccaa ggcgcataac ttaaaaaaca
     7861 tcgatgtcaa aataccgttg ggcatgtgga gctgtatcac cgggatttct ggttccggca
     7921 aaagcacatt ggtgcgcgag gtcctctaca agggagtcaa gttgctgttg ggccaatttg
     7981 ccggccggcc gggggcgcac aaagagatcg ccggatggaa agccatcgaa cgaatcgttg
     8041 aagtcgatca gacgccgatc ggcaaaacgc cgcgctcggt gccggcctct tacgtcggct
     8101 tgctggacga gatccgcaaa ctctacgcgc tgtcgcccga ggcgcggctg cgcggttata
     8161 cgccgagccg gttctcgttc aacgtcaaag gtggacgctg cgaagcctgc gccggccagg
     8221 gcaagatccg caaggagatg agttttctgc cggatgtttt tgtcgactgc gatgattgcc
     8281 gcggcgaacg gttcaacgaa gaaaccttga acattcgttt caatgggcgg aatatcgccg
     8341 acgtgtttca catgaccgtc gaagaggccg tgccgttttt ccaagccttt ccgaagattg
     8401 cgcggccgct caaagtttta gacgatatcg gcatgggcta tgtcactctt ggacaggcga
     8461 gcaacacgct ctccggcggc gaggcgcaac ggatcaaatt ggcttacgag cttggcaagg
     8521 attcgcgcgg caaaacatta tatcttttgg atgaacccac caccggcctg cactttgccg
     8581 acgtcgacaa gctgattcgt attcttcacc gtctggtcga ccgcggtaac accgtcatta
     8641 ccattgagca caacctcgat atcgtcaaag acgccgacta tttgatcgac cttggccccg
     8701 agggtggcga gcagggcggc cgcctggtcg cggcgggcac gccgttgcaa gtcagcaaag
     8761 atggtcggcg ctcgtacacc gcccgttttc tgcgcgaata tctaaaccgc ggctccgaat
     8821 ccggcgagac agtttcacct ccgattcttg aacaagtcat cgcttgacat ggcccgccgc
     8881 atcctaccgc tgctccgata tggcctacgc ctcaaatgtc cgcgctgcgg cgtgggatcg
     8941 ctgtatgcgg ccgttttccg gatgcatacg acgtgtcctc attgcgggct cgtgttcgag
     9001 cgggagcaag gctattttat cggcgcgatc tacatcaatt atgccgccac cgtgctgatc
     9061 gcggtgccgg gattttttat catgagtatt ttcaccaacc tgaccatcga ccagcaactg
     9121 tggatctgga tttcgttcgc agttatcttt ccgttgcttt tcttccatca ttccagaagc
     9181 ctgtggttag tgctggatca tatcttcaat ccgcccgaga agttttacag cgtcccgccg
     9241 aagaagagtc agcggagctg acggttcgac tcgttcaagt cgctcaagtc gtcggatcct
     9301 aaaagtttcc cgacggattc cggagctgac catgacgtcc ggccgtcctg caggcggggg
     9361 tcggaaacgg ttagacattt tgaacgtatt gaactttttg aacggcttaa actctcaacc
     9421 aaccgtttat ccgatcgcca gcgccgctac tcccaggacc atcgcggatg cggcggctaa
     9481 ccggcgccgg ccctcacctt ccgcaagcag gcgagtgccc attgcggtgc cgatcagaat
     9541 gctgatctct cgcgctggcg cgacgtaact caccggcgta aactgcatcg cagtgagaac
     9601 cagaatataa gaaagcggcg ccagaacagc aaccccgatc gcttcaaagc ggtgctcgcg
     9661 ccattccacc cgggtctcgc gcggatagcg tgccggaaga gatgtcaaca aaactgtcct
     9721 gccgaggttg gagccccaat cgaggatcag aggcggcacc gcaaaccggc tcaccgcttg
     9781 cttgtcccac aacgtgtatg ccgcgatgaa caaacccgtg actgccgcat acagcaccgc
     9841 ggttttgact tcgctcccgc gcaagctttt tggactgccg gtaatcacga ccacaccgca
     9901 gatgatcagc aatgcgccgg cgacggcaag tgcggaaggt cgttcgccga gcaggaatat
     9961 agccgagatg gttgaaagca acggacctgt gccgcgtgcc agcggataga ccaacgaaag
    10021 gtcaccgaca cgatagcctt gattgaggag aacgaaatag gcgctgtgca gggccgcgct
    10081 cccggccatt agcgcaagct caaccacgcc gatttgcaga tgagtgacaa agatcactcc
    10141 gagcgccagc ggcgtataaa atatcgccga tagaacagcc accagccagg taaaggtcac
    10201 atgaccgcta gctcgcttgt tcaagagatt ccaactagcg tgaataaaag ccgccgccag
    10261 gattaaaata agcgcgccga aagtcatgag tgttgttgtg cgatggcccg tgacaaggac
    10321 taggaagacc catgaagtct ttgcttgttc ggagccgaca gatcaggcgt atacgaaccc
    10381 ccctactgga gcaagacagg cagttcaggc gcgatcatcg agaaaaactg catcgcgcta
    10441 tcgattcggc ttagagccgg acagttagtc ctcaaactca gttttcgcag gatcgaggaa
    10501 agagcttaac ccgtactagg aaaagaaccc agaacgacca cgccttttta tctggtcccc
    10561 ttctgagctc acgaccgtcc aatcggggat gaatttagtc gctaaaaacc gcacaaaacg
    10621 attgcaaatc atatgaacca tgaatagagt cgaggccatc gagattgttt ttggctattc
    10681 gcgctataga cgcgagccat ccctgcggtg gtaagcaaat ggtgatgaac cgagtccgct
    10741 cttctaacca accagcaacc cagctcgtat taatagcaat atgtttcacg gcttttcgcc
    10801 ttgtcgactt ttttacattg aaatgttagc cacaacggcc tctagtaggc ttattcgata
    10861 taagcgtgtt acttgaaggt tggcaatcgt tgtagctagg aatctgcttc cctcacgatc
    10921 ttcgatctcg ctgcggattt gggaccatga tgcttccgct gcatgtatcg gagctgtcaa
    10981 cataggtgaa cagatcggta gctgtaacag aatgacatcg agatttggcg aggaatcttt
    11041 aacagagact gcgcccgtct gttagtgaag gcctttcggg aggacaggat ggataccgcc
    11101 ctaccaaata gaagcaaata cgtagaagga tttcgttatc aagaggattt ccgtattcag
    11161 ttgctccttc tcgctctttt gatcggcata gcctattacc taggcgctct acttagcttt
    11221 acgctgcggg tcccatcgac acgcagttcg atcctttggg tacccaacgc gatacttctc
    11281 gcggctttcg ttgtcacacc tgtaaaaaga tggtggctct ggatcttggc tgcggttccg
    11341 gcccatctct tggcgcaatc acgggatgcc gcaccggttt tggttctcct ctgccctttt
    11401 cttgctaacg ttgctcaagc ggccctcgcc gcagtcagcc tgcgcctttt aacggacgtt
    11461 ccgcaccggc tcgaatctct tcgcagcatg ggtatcttca tcctcgtcgc agtgattgcg
    11521 gctccggccc ttgcgtcatt tacctgtgca tggctcttcg tgctcgccgg ttgggagacc
    11581 gattactggc tggtgggaca ggcgcgctgg ctaaataacg ttgtaaccgg tcttacggcg
    11641 gcacctctat gcctcgcgat cgcaggcggc gatctgtaca aacttcgttg cctgcaggtt
    11701 cggcgttatg gagagttcgc agtcgttata cttggtctcg tcctcatact ctcgctcact
    11761 tccgcatggc acgccgccga aacgatcgat ttccctattc ggctctatgc tccgttgccc
    11821 tttttgctat gggcggcggt ccgttttgga cctccgggct tagcgatcgc ccttttggct
    11881 gtcgcgtacg actccatatc tgattccatt tttggtcaag ctccttcggc cgcgagctcg
    11941 ccggcgatca atgtgctggc tctggaggcg tcgctcggcg ttctctccct tcccttgatg
    12001 ttgttagcag cgcttatgat ggagcgacag cacaaagacg aggccttgcg tgagagccaa
    12061 gagcgtttag cccggacgga aaagttctcg ctggtcatgg taacacacac ggacctggag
    12121 ggccgctggc tcaaggtccc tccgacactc tgcgagttgc tcggttataa cgagcaagag
    12181 ttgctcgggc gtcgctttga tgagctaacg catcccgacg atgtagagat aaatgtaacc
    12241 cgacgcctgc agcttctgcg gaacgagatt aagtcattcg atctcgaaaa acgctatctg
    12301 cgaaaaaacg gcatggtcgt ctgggtatat atcaacgtgt cagttgtgac ggatcgacac
    12361 ggaagtcccg tccatttcct gagctatatt cgggacatca gccgtcgcaa ggaggctgaa
    12421 cagggactaa gggagagcga agaacggttg cagcttgcgc tcgaagccgg cggaatgggg
    12481 gtttgggatt gggatacgtg taacaataca cttgactggt ccagcgagca gtttacgatt
    12541 atgggcttta aaccgtttag catcagacca acataccaga gctggaccga gcgcgtgcat
    12601 ccggatgatc gcgcgcacgt cagcgcagcc atcgaacggg cgatcgctga acagagccac
    12661 tatcgttccg aatatcgcat agtcttgccc gacgggagcc atcggtggct gatctcccgc
    12721 ggcgaggcag tgtataaaac cggcggtgaa tacgtcaggc tggcgggtgt gaccgtggat
    12781 attacggatc gcaaacgggc cgagtcacgt ttgaacgttc aataccagat cgcgcgcgtg
    12841 ctatcgaatt ctgcttcgct taccgatgcc gcgccggcgt tgattcaaag gatctgtaat
    12901 tcttttgatt ggcattgcgg cgagatttgg caacccagcc ctgcggcgga cgtgttgacg
    12961 tatcttgaag gttggcattc gccatcgatg aagttgaccc cattcgtctc cgagagccgg
    13021 cgatatactt gctcgcctgg aatgggactt acagggcgcg tctggaaaag tgggaccccg
    13081 gcctggatca tcgatatcgc cgacgatgat aattttccgc gcgcttccat ggccgaaaaa
    13141 tccggtctga aaagcgcatt cagttttcca attactgtcg gcgatcgcac gctggcggtc
    13201 atggcgtttt tcagcaccga cgtgcgacag cctgacgaag aattgctcca gacaatggac
    13261 agtatcggac ggcaaatcgg ccagttcata gagcgtaaga acgcggaaca ggtcacgttc
    13321 cagcgggacc agcttctcca tactatgttt gaatcgcttt cctctcacgt tgtggtccta
    13381 gattgcgacg ggacaataac gtatgcgagc aaattagccg agcagttcac gacaagtcag
    13441 ttgttgaaca acctctcggt tggaattaac tatctggacg tatgcaggcg ggccgcgagt
    13501 ctcgaagacg ggaatgccaa agaggctctc tgtggaattg aggacgtact tacaggacaa
    13561 ctctcaagct tttctatgga gtatcgttgc gatgcgaacc aatgtgcgcg ctggttcgcc
    13621 atgcaggtag atcccatgcc gcgcgaccag ggcggcgtcg tcatcagtca tgctgatatc
    13681 acagagcgca tgcaagtgga tgaggcgctc cgcgatgcat tgaacgaagt gcgtaaactc
    13741 aaagagcagt tagaactcga gaatacctac ctgcgcgaag aagtgtccga agtccatcgg
    13801 tttggcgaaa tcggcggacg cagtgagtcg atctcgaaag cgcttcgaca tgcggaattg
    13861 gtcgcgccaa ccgatgcggc agtactcatc atgggcgaga ccggaacggg taaagaacta
    13921 ctggctcgcg cagtgcatgc gcgcagcaaa agaagcgaca gaccgttggt aaaagtcaac
    13981 tgcgccggtt tgccggcgac gctcatagag actgagctgt tcggtcacga gaaaggcgct
    14041 tttaccggcg ctgcggccag acggattggc cgtttcgagt tggccgatgg agcgacgatt
    14101 ttccttgacg aagtcggcga actgcctctg gaacttcaag ccaaattgct gcgtgttttg
    14161 caggagggtg agttcgagcg cctcggaagc tcgaagacta tgacggtaaa cacgagagtg
    14221 attgcggcta ctaatcgcga cctcgggctg gcggtcaagg ccggaacgtt tcgggatgat
    14281 ttgtactatc gattgaacgt atatccgata aagctaccgc cgttacgtga tcggaaagag
    14341 gacataccgg agcttgtcca aacgtttctc agggaagcca gccgcagact tggtcgacct
    14401 ctcgatggtg tatcccaacg cgtgatggac gcttttatga agtacgattg gccgggtaac
    14461 gtccgtgagc tgcagaatgt gatagaacgt atgacggtta cagcgaaagg cgcccagcta
    14521 agccttcctt ctgattggtt gactggtggt tcgctgaccg atatgccttt tgatctattc
    14581 aaaagccagg aagctcattg cgctgtgtca tcggaagaag aaacgaccgt ggaaaacgtt
    14641 gaacgcgctc atatccttag aattctcgag caaagacatt ggaggataga aggtccc
//
LOCUS       DASUVS010000299         5612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1215366, whole genome shotgun sequence.
ACCESSION   DASUVS010000299 DASUVS010000000
VERSION     DASUVS010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215366"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(436..2043)
                     /locus_tag="VF182_16180"
     CDS             complement(436..2043)
                     /locus_tag="VF182_16180"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX6438677.1"
                     /translation="MPNFIDFCADYFEKYFRLHPAEAIYYGVTGYDHLLNDYSDETYD
                     AQKRLVNDSLETLRQIPANDLDADQAIDYALLEGRLSIQKYEQEKEDYRLKWPDHYLA
                     TDAIYILTVRETDDLEKNLLSRLRGATDLVRQGISNLSRPEANPPRLWTEMSIQAAKG
                     GTTFLETLPNHPKVQAAIKDNAILRAGIERAKSAILDYAGFLTEDLLPRSRGVYAVGE
                     EHYNLLLRKKHFLDLDASALLNMGENLFWQTRRELTELAEELAPGIGVEEVTRAIQDK
                     HPASKEILPAYQKAMESARQFVKEKNLVSFPPREELHVVHTPEFRRHEIPFAAYLSPS
                     PKDPNQVGYYYVTPVADDELLREHNWVGLENTSVHESYPGHHLQFSLANSIPAASTLP
                     RLMNESSVFYEGWALYCEQLMQEQGFLKSREHRFVMLKDRLWRALRIIVDVKTQTGAM
                     TYDEAADLMVRELHFPRAQACGDLNWYSQSPAVPMGYALGWSIINRMRKQETARLGAR
                     FELRDFHDRLLGAGGISLPLVERRHFA"
     gene            2269..2682
                     /locus_tag="VF182_16185"
     CDS             2269..2682
                     /locus_tag="VF182_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX6438678.1"
                     /translation="MANRWLFKTEPSVYSFQQLLKDKKTVWDGVANNLALKNLKDIKK
                     GEQIFIYHTGDEKAAVGVAKALGGAYSDPSKNDPKLLVVDIEAGKPLAKPVTLAEVKA
                     HPKLKNFDLVRNSRLSIMKVTDEQWEIMEGMAKSK"
     gene            2814..3776
                     /locus_tag="VF182_16190"
     CDS             2814..3776
                     /locus_tag="VF182_16190"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6438679.1"
                     /translation="MGKMAEQGKHDHDHQLGDDKHAGQHDHSHGQHRAHDHEHSHDRG
                     HSHEHGHSHGHDHKTRFHDPAHAAEFDRRSTMEGIRGSLTTKMIETLSLSGNEWVLDV
                     ATGTGRVARPVSKHIKTGRIIGVDQALAMLDVGHQHKEPISSYFQSGGAADALPLKSN
                     SFDRAFVSFSLHHFGNPSGVVKEVMRVLKGGGRFVVLDPIIEEAKDAVDAALEAKINQ
                     VFRRTHGPEFRFHTASSIQRLLSKAGYRIPRTNILSYSFNQEGMDGIPTGRHWLEAAL
                     ELETEAPDLAERMKKNYFTWHAHGDHTHVKGSFSYALITGVKPE"
     gene            4264..5175
                     /locus_tag="VF182_16195"
     CDS             4264..5175
                     /locus_tag="VF182_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEX6438680.1"
                     /translation="MAVPDFQSIMLPLLVLMGDGAEHALAEVIETLGDRLRLSAEEKK
                     ELLPSESDVKFDNRVRWAAFYLRKAGLLERTGRGKIRISERGEQVLKTKPGRIDVKFL
                     KQFSEFQEFQKGTKRAESEDVNVPSEEAQSTPEETLEASYVNLRQTLAEELIDRVKKC
                     PPKFFEKLVVDLLVSMGYGGSRKDAGQAVGQSGDDGIDGIIKEDKLGLDVVYLQAKRW
                     EATVGRPVVQAFAGSLEGQRARKGVLITTSQFSQEAKEYVSRIEKKIILIGGEELAQL
                     MIDHGIGVTEVRTYSVRKMDLDYFGEE"
     gene            complement(5339..>5612)
                     /locus_tag="VF182_16200"
     CDS             complement(5339..>5612)
                     /locus_tag="VF182_16200"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX6438681.1"
                     /translation="SRLIGVIEAEQTENGKTFRNDRLIAVTAKSITHRSLGDISDVSG
                     DLVGQIEHFFISYNMARGKTFEPKGRYGRERALALVRTGIARADRA"
BASE COUNT         1372 a         1425 c         1513 g         1302 t
ORIGIN      
        1 cacgcccatt ggcgtgacga cttggtaaag atctgaactg cttcgcactg aaccttcgcg
       61 cccagcagca gggcgttgcc gatcccccct gcaatagaca tatgggcgcc gaggagcgga
      121 cggcgcggtt tttgtttttg catcataata ttcgaacggc ttgaacgatt gaacttcttg
      181 ataaattagc tgctccgacg attacggctg ttccactgca tccttcgcgg ttccgcgata
      241 attgtttggg attgattcac cgcagagacg cagagctcac agagcccgag tcacctttgc
      301 tgtcatacta acgcctaagg gagctcgcct tctcttatcc atgttttgtt ctttgtcgac
      361 ctctgcgtcc tttgcgcctt cgcggtgaaa acccgatcgt cacactcgtt caaaatcttc
      421 caaactttag gataatcacg cgaagtgtct tcgttcgacc agcggcaggg aaatgccgcc
      481 ggcgccaagc aggcgatcgt gaaagtcgcg caactcgaac ctggcgccca gtcgagcggt
      541 ttcttgctta cgcatgcgat tgatgatcga ccaacccaaa gcgtagccca tcggaaccgc
      601 cggcgattgg ctgtaccaat tgagatcgcc acaggcctgc gcgcgcggga aatgcagctc
      661 gcgcaccatc aagtccgccg cttcgtcata agtcattgcg ccggtctgcg tctttacgtc
      721 gacgatgatg cgcaacgcgc gccacagccg atccttgagc attacaaatc ggtgttccct
      781 gctttttaaa aaaccctgtt cttgcatgag ttgctcgcag tagagcgccc agccttcgta
      841 aaaaaccgag gattcgttca tgagccgcgg caaggtcgac gccgccggta tcgaattggc
      901 caacgagaat tgaagatgat gaccggggta gctttcatga accgaggtat tctcgagacc
      961 cacccagtta tgctcgcgta agagttcgtc atccgctacc ggtgtaacgt agtaatagcc
     1021 gacctggttc ggatctttgg gggatggcga gagatacgcg gcaaagggaa tttcgtggcg
     1081 gcgaaactcc ggcgtgtgga ccacgtgcag ttcttctctg ggcgggaaag agacgagatt
     1141 tttttccttg acgaactgcc gggcagactc catggctttc tgatatgccg gcaggatttc
     1201 cttactcgct ggatgcttgt cctgtatggc ccgtgtcacc tcctcgacgc ctatacccgg
     1261 cgccaactcc tctgcgagct cagtcaattc gcgccgcgtt tgccagaaaa ggttttcgcc
     1321 catattgaga agcgccgacg cgtctagatc gagaaagtgc ttttttctga gcagcaaatt
     1381 gtaatgctct tctccgacag cgtaaacgcc gcggctgcgg ggcagaagat cctccgtcag
     1441 aaaacctgca taatcaagga ttgcgctctt tgcccgctcg atgccagctc ggagtattgc
     1501 gttatccttg atggctgcct gaacctttgg atggttgggc agcgtttcca gaaaggtggt
     1561 gcccccttta gccgcttgaa tggacatttc ggtccacagc cgaggcggat tggcttccgg
     1621 ccggcttaga ttgctgattc cttgccgtac caaatcggtc gcgccccgca ggcggcttaa
     1681 aagatttttt tccagatcat cggtttcacg gacggttagg atgtagatgg cgtctgtcgc
     1741 aagatagtga tcgggccatt tcagtcggta gtcctctttc tcctgctcgt atttctggat
     1801 gctaagacgg ccctccagga gagcgtaatc aatggcttga tccgcatcca ggtcattagc
     1861 tggtatctgc cgcaaggtct ccagcgaatc gttcaccagc cttttctgcg catcgtaggt
     1921 ctcgtcggaa taatcgttca acagatgatc gtaaccggtc acgccgtagt agatcgcttc
     1981 agcgggatga agacgaaagt atttttcgaa ataatcagcg cagaaatcaa tgaaattagg
     2041 catggaagtc gactatcatc ttattggatg ccgcgcaatt ttttcaataa tattggcgga
     2101 gtcgttaggc gcagtatcca ggcggcatag ctctcaatct tgattgtgcg acaatcggga
     2161 accatggtga gatggagccg tggccatagt tcgcgttccg gctcgagtgc catgccatgt
     2221 ttgtattaaa gccagctgct gatctataaa ctatgcagga ggatttcaat ggcaaaccgc
     2281 tggttgttca agactgagcc aagtgtttat tcctttcaac aattgctgaa agacaagaaa
     2341 acggtttggg acggggtggc caacaatctc gcgctaaaaa atctcaagga tataaaaaag
     2401 ggcgagcaga tattcatcta tcatacgggc gatgaaaagg cggcggttgg tgtggcaaaa
     2461 gccttgggcg gcgcctattc cgatcccagc aaaaatgatc caaagctgct ggtggtcgat
     2521 atcgaagcag gcaagccgct agcgaaaccg gtcactctcg ccgaggtgaa agcgcatcca
     2581 aagttaaaga acttcgatct ggtgcgaaac tcccgtttgt cgattatgaa ggtgaccgat
     2641 gagcagtggg aaatcatgga aggaatggcc aagagcaaat aggacgagcg acaggcacca
     2701 gtaatacgga gcaaatgagt ccgactcggc gaaatctgcg tctctgcggt caatgatcgg
     2761 atttcaacgc agaggcgcga aggacgcaga ggtatgcaaa ggaagaaaca aggatgggga
     2821 aaatggcgga acaaggcaaa cacgatcacg atcatcaact tggggacgat aaacacgccg
     2881 gccagcacga tcattcccac ggtcagcatc gggcacatga tcacgagcac tctcacgatc
     2941 gcggtcattc ccacgagcac ggtcacagcc atgggcacga tcataagacc cgcttccacg
     3001 atccggcgca cgccgccgaa ttcgatcgcc gttctaccat ggaggggatc cgcggctcgc
     3061 tgacaactaa aatgatcgag acgctatctc taagcggtaa cgaatgggtg ctcgatgtcg
     3121 ccaccggcac cggtcgcgtc gcccggccag tttccaagca catcaaaacc ggtcgtatta
     3181 tcggtgtcga tcaggctttg gcgatgctcg atgtcggtca ccagcacaaa gagccgatat
     3241 cgtcctattt tcaaagcggc ggcgctgccg atgccttgcc gttgaaatcc aattccttcg
     3301 atcgggcgtt tgtctcgttc agcttgcatc acttcggcaa tccttcaggg gtcgtcaaag
     3361 aggtgatgcg agtgctcaag ggcgggggca ggttcgttgt gctcgatcca attatcgaag
     3421 aagccaaaga cgcggtggat gccgctctgg aggcaaaaat caaccaggtc ttccggcgca
     3481 cgcacggccc cgagtttcgc tttcatacgg caagcagtat tcaacgtctg ctgtccaaag
     3541 ccggctatcg tattccacgc accaatattt tgtcttactc gttcaatcag gaaggaatgg
     3601 atgggattcc caccggccgt cattggctcg aagcggcgct tgagcttgaa acggaagccc
     3661 cggatctcgc ggagcgcatg aagaaaaatt attttacctg gcacgcgcac ggcgaccata
     3721 cgcatgtgaa gggtagtttt tcttatgctc ttatcacggg agtgaagccg gagtaacgat
     3781 cgctggttcc aaacgttcca atcgttccat ccgttccaaa cgtttcagaa ttacatgacg
     3841 ctactccagc aacactcatc gacttcaacg attggaacat ttggaacaac tggaacggcc
     3901 ttcgcgggcg ggcgcggctt tactgaggga ggtcactgac aatgaagaaa gcagattttc
     3961 gcacattcta tcgtgatgcc catgccgtcg agtgcctgac cgaccgtgag aaaatactta
     4021 tgggtctcgc cgtagcgatg atgcggctgt tgacgtgatt gccgcggtgg atgccggagt
     4081 gctgcaatcc ttgcatcagc ggatcaaaca acgaaccaac agctagaaac tagacaatat
     4141 gcggcgcaac acggcgccac aaaaacgtgc cgtctgatcg atgatgggcc acgaattttc
     4201 tcccttagca tttctcctaa gaagctctga tccaagctat ccctatgatt tacgtgtaac
     4261 aaaatggctg ttccagactt tcaatctata atgttgccgt tgctagtgtt gatgggcgat
     4321 ggtgccgagc atgctctcgc agaagtgatc gaaactctcg gagatcgtct tagattatca
     4381 gccgaggaaa agaaggaact cttgcccagc gagagtgatg ttaaattcga caatcgggtc
     4441 cggtgggcgg cgttttatct acggaaggct ggcctgctcg aaagaaccgg tcgtggaaaa
     4501 atcagaattt cagagcgcgg cgagcaagtc ctaaagacaa agcctggacg aatcgatgtt
     4561 aaatttctta aacaattttc tgaattccag gaatttcaaa aaggcacaaa gagagccgaa
     4621 tcagaggacg tgaatgtccc tagcgaagag gcacagtcaa ctccagagga aacgttagaa
     4681 gcaagttatg tgaatctaag acaaacattg gcagaagagt taatagatcg agttaaaaaa
     4741 tgtccgccga agttctttga gaaattggtc gtcgatttgc tcgtgagtat gggttacggc
     4801 ggttcaagaa aagatgctgg tcaagctgta ggccagagcg gcgacgatgg aatagacggt
     4861 atcattaaag aggataaact tggcctagat gttgtttatc tccaagcgaa gcgatgggaa
     4921 gcgacagttg gacgaccggt cgtccaggca tttgccggga gtctggaagg gcagagggct
     4981 agaaagggcg tgttgataac aacatcgcaa ttttcgcaag aagcaaaaga atatgtcagt
     5041 cgcatcgaaa agaaaatcat actaatcggt ggagaagagt tagcccaact gatgatcgat
     5101 catggtatcg gcgttacgga agtcagaacc tacagtgttc gtaaaatgga cttggattat
     5161 ttcggtgagg aataaaattg gctgtccatt ccgtttgtcc gagctaagcc gacgattcgt
     5221 cagcggtgtg ccgctcacta cgacccgaaa tgacagcttt gagggcgcca caccgcagtt
     5281 tctttgtgca tttttttgtc gggccgaccg gagatcgata cagttgccca cgcttcgatc
     5341 aagctctatc agcgcgcgcg attcccgtcc ttaccagcgc caacgcccgc tcccgtccgt
     5401 aacgcccctt gggctcgaac gtcttgcccc ttgccatgtt gtaggagatg aagaagtgct
     5461 cgatttggcc gaccaggtcg ccgcttacat cgctgatgtc gccaagcgac cgatgcgtga
     5521 tcgatttcgc tgtaactgcg atcagacggt cgttgcgaaa ggtcttgccg ttctccgtct
     5581 gctcagcctc gatgacaccg atcaagcgtg at
//
LOCUS       DASUVS010000300         6765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1215389, whole genome shotgun sequence.
ACCESSION   DASUVS010000300 DASUVS010000000
VERSION     DASUVS010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215389"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VF182_16205"
     CDS             complement(<1..953)
                     /locus_tag="VF182_16205"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438682.1"
                     /translation="MKARMSTIFVATVFSVISLYAVAQSVNAQVRVSVGVTETMETFN
                     PYGDTVSLMFGVWCQVMGCLGTFDFVKGEYVGMLAERWEVKDSNNWVFHLRKNVKFHD
                     GSPLTAADVVHSFNRMKSDPHTKQTQYAVPIATVTAIDNHTVRFTTHKPTAPLLEYLF
                     DRFAITSKTIYDKHGPDIADRKYPFGAGPYKFRELIPGQRLVIAKNPEYPGIRTKKQA
                     PDEVVFRIMREPEQRVTALLAGEIQIAQFIPPHLRERVEKNPNTRVSAVNSVEMMFLA
                     MSPKFKPWDNKLLRQAVAHAIDRETIIATLLQGQASRLDGP"
     gene            1149..2144
                     /locus_tag="VF182_16210"
     CDS             1149..2144
                     /locus_tag="VF182_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX6438683.1"
                     /translation="MNDQEPNRGTKARVHELIASPNNVHWGYFSAALRPALTIDPGEI
                     VVIEDVPRVDPQIVERSGVVPVADITPNHRLIYKEVNDRGPGPHIMVGPVYINSAEPW
                     DVLEVRILDVRLAYNYGYNTQRPYQATLPEEFPRFWQRIIPIDQKERTAKVAKDVVVP
                     LSPFFGTMGVAPPVSLGRITTVLPGVHTGNIDNKDVGANTVLYMPIHVHGALFSAGDG
                     HANQGHGEVDGTAIETGLRGRFQFFVRKDMRLKWPRAETSTHWIVMGLHEELDQAMKM
                     AVHETIDFIVGRFPHLTPEESYMIASVAVDYHVTQVVDGRKGVHGMIPKSIFIKT"
     gene            2258..2356
                     /locus_tag="VF182_16215"
     CDS             2258..2356
                     /locus_tag="VF182_16215"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX6438684.1"
                     /translation="MPTKSGFEEVRTRGSHVIMQKKSVRKDYCSSA"
     gene            2962..3336
                     /locus_tag="VF182_16220"
     CDS             2962..3336
                     /locus_tag="VF182_16220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6438685.1"
                     /translation="MPIRVLLVEDDTLTRRNTALYLRRAQIEVDEAADGNEAIRLITS
                     SDQYDVIISDLRMPGRVDGMDIITHQQRVSPGTLCILVTAFGSDQVQRRAQDLAVTYL
                     EKPISLPDLRSKVTAFASRHTP"
     gene            3435..4604
                     /locus_tag="VF182_16225"
     CDS             3435..4604
                     /locus_tag="VF182_16225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6438686.1"
                     /translation="MGFWSTIRRWIGFLRSKRSAPASQNSHYDPTTERLRQSEERFQI
                     LIDSIQDYAIYILDPEGFVISWNSGAVRIKGYSAQEIVGQHFSRFYTRDEIRIDKPRQ
                     NLAIAAETGKYEDEGLRMRKDGSLFWANVLITAIRDSSGTLTGFAKVVRDVTERRASE
                     QRLYENERLATLGTTAAVFAHEVANPLNGISGSLQIVRELLDDADYQNPVLRETIAAA
                     NQEIQRLTSLLGDYRSLARPQILNLRASSLRQLVEQVLAGNTRNYQNLGISLSVDFDE
                     DLPLVPVDQEKIKQVILNLCKNAVEAMPNGGLLIVRAYRSSDHVVLEVSDTGHGIPEG
                     FEPFHLFKTTKPEGTGLGLPIVQQIITDHHGTVDYISESGKGTTFRVSLGLLDHK"
     gene            complement(4683..5753)
                     /locus_tag="VF182_16230"
     CDS             complement(4683..5753)
                     /locus_tag="VF182_16230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6438687.1"
                     /translation="MIDIRSLTKIFKSGGDVVRAVDAVDLQVREQEFFVLLGLSGSGK
                     TTLLRCVAGLEKPEVGEIKLGDETVSAPERGIFIPVEDRALGMVFQSYAVWPHMTVFQ
                     NVAFPLANGKRRLARGKIRERVMGALELVQLAALADRPVPFLSGGQQQRVALARALAV
                     EPKVLLMDEPLSNLDARLREEVRDEIRLLATKLSITVLYVTHDQVEAMALADRIAVMS
                     GGKILQIGAPQELYDGPRDRRVGEFLGAMNEFEGSVQSDGRVKLDVGVLDCALPNGAK
                     DVIVAIRPEDVAVSRECSGASNEFAAELLSKTFLGDVTIYNLSIGGTKLRGKTTQADE
                     KLVPGSKAHVRLPADKLKIFPK"
     gene            complement(5955..>6765)
                     /locus_tag="VF182_16235"
     CDS             complement(5955..>6765)
                     /locus_tag="VF182_16235"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6438688.1"
                     /translation="RWWLLGWAMLGLYFTLAFILPLLAIFWVSLLPYVQIPSRQALDS
                     ISFDRYSALALDTGLIQAGLNTLLLMVVVPTLIVTICAAISWIVTRSRLRWRVALDAV
                     AFLPHPVPNLLFALAIAYFALLISNVVPVYGTIYVIVAVYVVCWVSFGTRVLNNSMIQ
                     LHRELEEAAQVGGVSTLRILAKVIVPLIRPGIVYAWIWTSLSAYRELTMAVFLASPKS
                     QVLSTYIWGQWHGGGLGDAAAIAIIMMAVMAPLVTTFWIYARRLQHVASSA"
BASE COUNT         1641 a         1796 c         1750 g         1578 t
ORIGIN      
        1 atcggtccat ctaaccggct cgcctgacct tgcaggagag tcgcgattat cgtctcacga
       61 tcgatagcat gggctacagc ctgacgcaga agtttattat cccagggctt gaatttagga
      121 ctcatggcta aaaacatcat ctctacagag ttcacggcgg aaactcttgt gttgggattt
      181 ttttcgactc gctcgcgcaa atggggcggt atgaattgag caatttggat ttccccggcg
      241 aggagcgccg taacgcgttg ctcgggctcg cgcatgatac gaaagacgac ttcgtcggga
      301 gcttgctttt tagttctgat gcccgggtac tccgggtttt ttgcaataac caggcgctgc
      361 cctggtatca gctccctgaa cttgtaaggg cccgccccaa acgggtactt ccgatcagcg
      421 atatccggac cgtgcttgtc gtaaattgtt ttgctcgtga tggcgaatct atcaaagagg
      481 tactccaaga gcggcgcggt gggcttgtgg gtcgtaaagc gcaccgtgtg attgtcgatc
      541 gcggtaacgg tggcaatggg gacggcatat tgtgtttgtt ttgtatgtgg atcgcttttc
      601 atgcggttaa acgagtgcac gacatcggct gctgtgagcg gcgagccgtc gtgaaattta
      661 acattcttgc gaagatgaaa gacccagttg ttcgaatctt tgacttccca acgttctgcc
      721 agcattccta cgtattcgcc tttgacaaaa tcgaaagtgc cgaggcaccc catgacctga
      781 caccagacgc caaacattaa agacacagtg tcaccgtagg gattgaaagt ctccatcgtt
      841 tctgtaaccc ctaccgacac acggacctgc gcgttaacgg attgagcgac ggcatacaga
      901 ctgattaccg agaaaacggt tgccacaaag atcgtactca ttcgtgcctt catcgataaa
      961 cttcctttca tgcgcgtcgt ttaattggag cgggcggaaa aattacgggt aaacagcctc
     1021 cttataaagc cgctatctat tttctgtcaa tcttcgtttc tcttttgcct gacgggccgc
     1081 cggacatctt ttcagcggcg gttttacaag gtataaccga gaactcgaaa cgcacaggag
     1141 ttgtatgcat gaatgatcag gaaccaaatc gcggcaccaa agcgagagtc cacgaactca
     1201 ttgccagccc taacaacgtg cattgggggt acttcagcgc tgcactccgg ccggcactga
     1261 caatcgatcc gggtgagatc gttgtcattg aggatgtccc gcgagtggat ccgcaaatcg
     1321 ttgagcggtc cggggtagta cctgtagccg atattacgcc caatcaccgt ctgatttaca
     1381 aggaagtaaa tgaccgaggt cctggaccgc acatcatggt tggcccggtt tacataaact
     1441 cagctgagcc ttgggatgtt cttgaggtcc gcattctcga tgtccgcctc gcctacaact
     1501 atggctataa cacccagcgt ccttaccagg cgaccttgcc ggaagagttt ccacgattct
     1561 ggcagcgaat catcccgatc gatcagaaag aacgtacggc taaggtcgct aaagacgtcg
     1621 tggttccgct gtctcctttt ttcggcacga tgggggttgc gccgcccgta agtctggggc
     1681 ggatcacgac ggttcttccc ggggtacaca ctggaaatat tgacaacaag gacgtgggtg
     1741 caaacacagt tctctatatg ccgatccatg ttcacggtgc cctgttttcc gcgggggacg
     1801 gtcacgcgaa tcaagggcat ggtgaggttg atggaacggc catcgagaca ggcttgcgcg
     1861 gccgtttcca gttctttgtt cgaaaagata tgaggcttaa gtggccaaga gcggagacgt
     1921 ctacgcactg gatcgttatg ggactacatg aagaattaga tcaagctatg aagatggcag
     1981 ttcatgagac tattgatttc attgtcggtc ggttccctca tttaacaccc gaggagagct
     2041 atatgattgc cagcgttgca gtcgattacc acgtgaccca agtagtcgac ggcaggaagg
     2101 gcgtgcatgg aatgattccc aagtccatct ttatcaaaac gtgaaagtga tcgtcctact
     2161 gtaagtcaaa cagcggctgc gcagcgcggt gttcgtaacc caaatttagg tagcggttgg
     2221 ctaggttgcg cattctttcg agaaggaagt ctgtgctatc ccaacaaaat ccggctttga
     2281 ggaagtccgg acgcgaggca gtcacgtcat catgcagaaa aaaagcgtca ggaaagatta
     2341 ctgttcaagt gcctgacggt aaaattcgca tcggcaacct gcaatcgatc atccatcagg
     2401 ccggcgtagc ccgtagcgag ttcgaggagt gattacccaa tcaattttgg atttccgatt
     2461 ttggattttt gattccgcct ggatcaacac tgaatataaa aatcacttcg cgccgcgagt
     2521 ctttgaatgc gagttcggtc ttcatttctc aacaaagttc aaattccgac cgggatcggt
     2581 tccgaccaac aacgcctgat tcaaaccgtt ccagccgttc aatcgctagg ctcagttcaa
     2641 attgttccaa gaattcggga attctcgagt gcgaatgccg cgacaatcgc ttctgcggtg
     2701 cgcttgttga aaatcggtga cgcaaagcaa ttcacataac gaacttaact ccagtcggtc
     2761 gataatctgt accgctggag gcaatctgac tctgtcgacc gctactattg gcggctcaaa
     2821 tcttttttac gtctttgctc atgggattga tgagcacggc cgagagataa aacatggggc
     2881 cactaacccc aacgacccat cttgacccag gcaatcagac tcaataagat tctaacagct
     2941 aaactttgag cgacaacggc gatgccaatc cgagttcttc tcgtcgaaga cgacacactt
     3001 acccgcagaa ataccgcgct ctaccttcgc cgtgcacaga tcgaagtgga tgaggcggct
     3061 gatggcaatg aagcaattcg gctgattact tcatccgatc aatacgacgt tattatctcc
     3121 gatctgcgga tgccgggtcg ggtcgatgga atggacatta tcacgcacca gcagcgagtc
     3181 tcgcccggta cattatgtat tctcgttacc gcgtttgggt ccgatcaggt tcaacgacgg
     3241 gcgcaagatc tcgctgtgac gtatctcgag aagcccattt ctcttcctga tttacggagc
     3301 aaagtcacag ctttcgcaag tcgccatacg ccctaaggcg gccgtgcagc cgatcattga
     3361 gtccgcctga ttggacgatt tataaattgc ttcgaaaaac tctcaccatc tcaaacaatc
     3421 gatttccgcg cgccttggga ttttggtcga cgataaggag atggattggt tttcttcggt
     3481 caaaaaggag cgctcccgca tctcagaact cccattacga tccgacaacc gagaggcttc
     3541 gccaaagcga ggaacgtttt caaattttga tcgacagcat tcaggactat gcaatctata
     3601 ttttggatcc cgagggattt gtgatcagtt ggaactctgg cgcggtacgc atcaaaggct
     3661 atagcgcgca agaaatcgtc ggccaacact tctcacgctt ctatacacgg gacgagattc
     3721 gcattgataa accgaggcag aatttggcta ttgctgcgga aacgggaaaa tatgaagacg
     3781 aaggcctccg catgcgcaag gacggatccc ttttttgggc taatgtgctg atcactgcga
     3841 ttcgtgattc ttccggaact cttacgggtt ttgccaaggt tgtgcgtgat gttaccgagc
     3901 gcagagcatc cgagcaacga ctttatgaga acgagcgttt agcgacttta ggaacgaccg
     3961 ctgctgtctt tgctcatgaa gtcgccaatc cactaaacgg catttcaggt tctcttcaaa
     4021 tcgtgcgcga actgttggac gatgcggact accaaaatcc ggtcctgcga gagacgattg
     4081 ccgcagccaa tcaggaaatc cagcgcctga catcgttact cggagactat cgatcgcttg
     4141 cgcgaccgca aatcttgaat cttcgagcga gcagtttaag gcagttagtc gagcaggttc
     4201 tcgccggcaa cactcggaat tatcaaaatt taggcatttc actgtcggta gattttgatg
     4261 aggacctgcc tctggtgcct gtcgatcagg aaaaaatcaa gcaagtcatc ctaaaccttt
     4321 gtaaaaacgc ggttgaggct atgccgaacg gaggcctgct aattgtccgg gcttaccggt
     4381 cgagcgacca cgttgtcctt gaggttagcg ataccgggca cggaataccg gagggctttg
     4441 agccttttca tctcttcaaa accaccaaac ccgaaggtac agggttagga cttccaattg
     4501 tacagcagat cataacggat catcatggaa cggtggacta cattagcgaa tcaggcaaag
     4561 ggactacctt cagggtttcg ttgggacttc tagatcacaa gtgagctttg gttcaagcac
     4621 gtcggtttgc ttggggatca attttgattt atgcagttag ccagtacccg gcgttctctc
     4681 ggctattttg gaaaaatttt taacttgtcg gccgggaggc gcacgtgcgc tttgctcccg
     4741 ggcaccaact tttcgtccgc ttgagtggtc ttgccacgca gcttagtgcc gccgatcgat
     4801 agattgtaaa tggtgacatc gccgagaaat gtcttggaca gaagctctgc ggcaaactcg
     4861 ttgcttgcac cgctgcactc gcgtgaaacg gccacatctt cggggcgaat tgctacgatg
     4921 acatccttgg cgccgttcgg gagcgcgcaa tctaaaactc cgacatcgag ttttacgcgg
     4981 ccatcacttt ggaccgagcc ttcgaattca ttcatcgctc cgaggaactc cccgaccctg
     5041 cgatcccgtg gtccgtcgta tagctcttgc ggcgcgccga tctggagaat cttgccgcca
     5101 ctcataaccg caattcggtc ggccagcgcc atcgcttcca cttgatcatg ggtgacatac
     5161 agcacggtga tgctgagctt ggtcgcaagg aggcggatct cgtcgcgcac ttcttcacgt
     5221 aaccgcgcat ccaaattgct cagaggttcg tccatgagca gcactttggg ctcgaccgcc
     5281 agtgctcgcg ccagcgctac cctttgctgt tggccgccgc tcaaaaaagg cacaggccga
     5341 tcggcaagcg ccgccagttg aaccagctcc agggcgccca taacgcgctc gcgaattttg
     5401 ccgcgcgcta atctgcgctt gccgttagcc aggggaaaag cgacattctg gaaaactgtc
     5461 atgtgcggcc aaaccgcata agattgaaag accataccga gagcgcgatc ttcgaccggg
     5521 ataaaaattc cccgctccgg cgcgcttacg gtttcgtcac cgagctttat ttctccaact
     5581 tccggtttct ccaagccggc gacgcaccga aggagcgtcg tcttgcccga accgctcaga
     5641 ccgagcaaca caaaaaactc ctgctctcgc acctgcaagt cgacggcgtc caccgcgcgc
     5701 accacatcgc caccgctctt gaaaatcttt gttagtgaac ggatatcgat catttttgct
     5761 taaccggctg ctgaaaaggc ccaccgcatc aaaaggatga attatgaaag ctgaaagcag
     5821 aagtttccgt tttcagcctt ccgctttcat acttgttttt cccgcctccc atctgagacc
     5881 tttttgaaca gccgtgacgc agtttgtaaa tactctttag ccgtgaggct atactcacgc
     5941 ccgtcactcg tcttctacgc cgacgacgcg acatgttgca ggcgccgcgc atagatccaa
     6001 aatgtcgtca caagcggcgc catgaccgcc atcatgatga ttgcgatggc ggcggcatcg
     6061 ccgaggccgc cgccgtgcca ctgcccccat atataagtag aaagcacctg gcttttcggt
     6121 gacgccagga acaccgccat ggtcagttcg cgatacgcgg ataacgacgt ccagatccag
     6181 gcgtagacga tgccgggtcg aatcaacggc acgatcactt tggcgagaat gcgcagagtc
     6241 gaaactcctc cgacctgcgc cgcctcctcc agctcgcggt ggagttgaat catactgttg
     6301 ttcagcaccc gcgtaccgaa gctcacccag caaactacat agacagcgac gatgacatag
     6361 atcgtgccgt acaccggcac gacgtttgaa atcaacaagg cgaagtaagc gattgccaga
     6421 gcaaaaagca agttcgggac ggggtggggc aggaaggcga cggcatccaa tgcgacacgc
     6481 cagcgcaaac gcgatcgtgt aacgatccac gagatcgcgg cgcaaatggt aacgatgaga
     6541 gtcggtacca cgaccatgag cagcaatgta ttcaaacccg cctgaatcag cccagtgtca
     6601 agagcgagcg cgctgtaacg atcaaaggaa atcgaatcga gcgcttgccg cgatggaatt
     6661 tgcacgtaag gtaagagcga tacccagaaa atcgcgagca gcggcagaat gaaagccagt
     6721 gtgaaataca atccaagcat tgcccagccg agcaaccacc agcgg
//
LOCUS       DASUVS010000301         7465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1870683, whole genome shotgun sequence.
ACCESSION   DASUVS010000301 DASUVS010000000
VERSION     DASUVS010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870683"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VF182_16240"
     CDS             <1..550
                     /locus_tag="VF182_16240"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438689.1"
                     /translation="VINQRLDGIEKEMSAEMNSEGFKLGQVEFRRFFTIRYRRQSAGV
                     EMPVGWDRFNPKRMSEMHSAFEKKYEELYGVGSGYAKAGIEISEIRVDAVGKVAKPKL
                     TAKRKSNRDARSARKGKREIFFTRPERKFIDTPIYDYERLGAGASVRGPAVIELPFTT
                     ALVPPGFTVKVDPYLNLVMEIG"
     gene            710..1702
                     /locus_tag="VF182_16245"
     CDS             710..1702
                     /locus_tag="VF182_16245"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438690.1"
                     /translation="MKTNRVTYYRFCLAVAFAALLWPATSLVAQTVRIAVGTASVASL
                     PTWVAAEGGYFTREGLHAELIYIRGGPQTLSALLGGDVNFAQVYSGPILSARLTGADA
                     VIVAGLINQPLFSVVTAAGIDKPEDLRGKKVGISTYGSATDVALRLALKKWGLKPESE
                     VNILQMRGVPEILPALASGSIQGGVLSPPTNMMAIKAGFKELSYLPQLGISFQHTSLA
                     TTRRYLDKNRSTALKALKAYTAAIERIKADKPFAKRALSKYMQTNDNEVLEYSYNAAN
                     PVFKAPPYPTLDGIRATLDFLAEKEPKAKQAQAKEFADLSLLEELEKTGTTDKR"
     gene            1778..4000
                     /locus_tag="VF182_16250"
     CDS             1778..4000
                     /locus_tag="VF182_16250"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX6438691.1"
                     /translation="MIQKKPTIDPIRYEMYLHRLWAIGEEGRMTLQRVTASPIVSQGG
                     ECMSSFYDETGTMVLACSGHLRFAAATSDAIRNLMQRFSKTPGFHDGDQFFFNDPYVA
                     GSHTYDMMIVKPIFFRGELIAWTATSTHTADTGGVLRGGAYEIFHEGICIPGLKIVEK
                     NEFREDVFQTLTGQCRDPQYVGLDLKAMIAGNNICAKRYLGLVEKFGLKFVQLAGEKM
                     IADSESKARAKLRSMPDGTWMTRQYVTTLDRKTRKSVAQQLYCTMTKKRDELLFDFTG
                     TSPQTDTDHNSTLPSTVAHLALALTNTLFWDVPWSDGKMRPVKVHVPEGSILNCKYPA
                     ACGAGPRIGNVLVSTVCEAVAKMIYASGRYDDVNASTTGNLEFVGGPGYFYGGHTREG
                     IAVAQGLYDIHGAGMGARPHGDGVNTGGHMNIPSAGISDIERIEMQYPFLYFTRAHNP
                     DGSGFGQYRGGLGSYRIYLIYGSKDCSVDYKPYGGVAQGGFGLAGGYPTGIGATRVMI
                     EAGLEMLGRVKAGEYPTVAAMRAGEWGKSILPAGVPERVPLPEGTLLVDYVAGGGGFG
                     DPIDRDPAAVLKDFGYGWVSREIAERIYGVVFADGGKLVDGAATENCRREIREARRQD
                     APMSQKSNGLAGEVGRDSRPPLRFHAALEIVGNGKRKTIRCSRCGHLYCKGDENYKQH
                     ALHRIVDLNDVMPPLPTGERYIGEYHIYSCPGCATQLQVDLFSPSLGGDPVLWDTRIA
                     "
     gene            4138..5109
                     /locus_tag="VF182_16255"
     CDS             4138..5109
                     /locus_tag="VF182_16255"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438692.1"
                     /translation="MKLSRFARLLFAVILLASAAQEISAQDRISLGITTKTGSTGLPF
                     VIAEEKGFFKSEGLNAVSVVMQNQVVVNGVVARQVDYGGTFSNFVGAALSGLPVRIVM
                     AVMEGSDHYLVTSPNIKKVEDLKGKKFGISSFGGTPHSEAVMILRKYGMNPEKDVTFL
                     QIGGSSSRYTALDSGAIDAAMLVPPFNKLAAKRGFNEILSFNEIMNIPLGGLTVHTQR
                     IKEKPDEIVRMIKAMLKGVNYIRNNQAEILSIMASKWGIKEADVREGIYRDIVGIYTR
                     NGVASDETMKNVIQLVRDTRKSKDDLGLSDIVDWSFAKKAQAELKIR"
     gene            complement(5232..6212)
                     /locus_tag="VF182_16260"
     CDS             complement(5232..6212)
                     /locus_tag="VF182_16260"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438693.1"
                     /translation="MKLISAAFSALSLLWLLITRPEAADKLVADYGGLSGFQSTVWVG
                     KDLKIFDKHGLDVDVIMINGSARSVAALLGGSTQFATGSATGPIVAAARGADLKVIAA
                     SYNKFPYAFIVKPEIRTPKDLPGKKVNILNFGGSNDLALQLALREWGLKLPDIQVIIG
                     GDAPTRLASLMTGRTDATILSPPHLTKAVQAGYRVLADMGEMSANFTQSSLYIKGSFL
                     REDRDRVKRFVRAYSEAVHAIKNDRARTMKVFARRMRIDDAETLQSTYNYFAPRFSLP
                     PRVDLSGVRDTIKFYAEHNPELKNRTAEEFIDHSLLDDIEKEGFFKKLGS"
     gene            complement(6374..>7465)
                     /locus_tag="VF182_16265"
     CDS             complement(6374..>7465)
                     /locus_tag="VF182_16265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6438694.1"
                     /translation="KDAPRTPVPSNFFDPIKGAANYAEHLDEMAYCEELGLDGVVFNE
                     HHYSSYGTMPSPNLIAAALSQRTKRIKIGVLGNILPLRDHPVRVAEEYAMVDCLSNGR
                     LIAGFVRGIPAEYVWYGVNPEESRGRFQEAYDLIMTAWTNSVWSFEGEFYKLKDCAIW
                     PRPVQQPHPPIWIAARSAESIEWCVKRHLPCAQVYQTNGQIEDTFNYYRIKAKEDGWN
                     AKPDDFILCRHIYIDDSNKKAQEIAEPAMRYFFSVYNRGFNDAINQKAADQQKLLAKL
                     TSERSFNYFREGNRSRIDFSTLGWDELIKSGYMIAGDPDSVTRQIQDQMKQIGANHFM
                     GMFHIGNLAHQKVISSLDLFKKEIMPRLN"
BASE COUNT         1722 a         2004 c         2130 g         1609 t
ORIGIN      
        1 cgtgataaac cagagacttg acgggattga aaaagagatg agcgccgaga tgaacagtga
       61 agggttcaag ctcggccagg tggaattccg gcgtttcttt accattcgct accgccgcca
      121 aagtgctggc gtggaaatgc cggtcggttg ggatcgtttc aatcctaagc gcatgagcga
      181 aatgcacagc gcatttgaga aaaaatacga ggagctgtac ggggttggct cgggttacgc
      241 aaaagccggc atcgaaatca gtgaaattcg tgtcgatgcc gtgggcaaag tggccaagcc
      301 aaagctgacg gcgaagcgaa agagcaaccg tgatgcccgc tcggcgcgca agggtaaacg
      361 tgaaatattt tttacccggc ccgaacgtaa gttcatcgac accccgattt acgattacga
      421 gcggcttggc gccggcgcaa gcgtgcgcgg cccggcagtc attgagctgc cgtttaccac
      481 cgcgttggtg ccgccgggat ttacggttaa ggtcgatccc tatctcaacc ttgtaatgga
      541 gattggatag agtcgagtcg ccctcgaaac caagtgcgac tcgcgcgaag cggttcagtt
      601 tggcaacgtt ctgacgaacg ctgatatgaa ataccgtaga tggcgagctc tagatggttg
      661 tttttgctgg cccgctctat tgcaaccttc cgacaaggag tgactcggga tgaaaaccaa
      721 tcgcgtgaca tattaccggt tctgcctggc tgtcgctttt gccgctctgc tatggcccgc
      781 gacatcgttg gtcgcgcaaa cggtaaggat cgccgtcgga accgcaagtg tggcatcgct
      841 gccgacctgg gtcgccgccg aaggaggtta tttcacacgc gagggactac atgccgagct
      901 gatttatatc cgtggcggac cccagactct ttcggctctg ctcggcggcg acgttaattt
      961 tgcgcaggtc tacagcgggc cgattctctc cgcgcgtttg accggagccg acgccgtgat
     1021 cgtcgcgggt ttgatcaacc aaccgctctt ttccgtcgtc accgccgccg gcatcgacaa
     1081 gccggaagac ctgcgcggca agaaagtggg catcagcacc tatggttcgg ccacggatgt
     1141 ggcgctcagg cttgcgctaa aaaagtgggg ccttaagccc gaaagcgaag tcaatatact
     1201 gcagatgcgc ggcgtcccgg aaattcttcc cgcacttgct tccgggtcca ttcaaggcgg
     1261 cgtgctctcg cctcccacga atatgatggc gatcaaggcg ggattcaagg aactctcata
     1321 tctccctcaa ctcggcatct cgtttcaaca cacctcattg gccaccaccc gcaggtatct
     1381 cgataagaac cgctccactg ctttgaaggc gctcaaggct tacacggcgg ccatcgaacg
     1441 aataaaagcc gacaagccct tcgccaagag agcgctttcg aaatatatgc agaccaacga
     1501 taacgaagtg cttgagtaca gctataatgc ggcgaatccc gtgttcaagg cgcctccata
     1561 cccaacccta gacggtattc gcgcaacgct agatttcctg gccgaaaagg agcccaaggc
     1621 caaacaggct caggccaagg aatttgccga tctgtcgctg ctcgaagagc tcgaaaagac
     1681 cggcacgacg gataagcgat gaaataaagc aaacggttcc tggaatcgct tttaaagcgc
     1741 aagctatggg aaccgcataa aaagtagagg taaaataatg attcagaaga aaccgaccat
     1801 cgatccgatt cgttatgaaa tgtatctcca tcgcctctgg gccatcggcg aagagggccg
     1861 catgaccctg caacgagtaa ccgcttcgcc gattgtctct caaggaggcg agtgcatgag
     1921 ctctttctat gacgagaccg gcaccatggt gcttgcatgc tcggggcact tgcgctttgc
     1981 cgccgccact tcggatgcga tccgtaacct tatgcagcgc ttcagcaaaa ctcctggatt
     2041 tcacgatggc gatcagtttt ttttcaatga tccgtatgtc gccggctcgc acacttacga
     2101 catgatgatt gtcaaaccga ttttcttccg cggcgaatta attgcgtgga cggcgaccag
     2161 cacgcacacc gcggacaccg gtggcgtgct ccgcggcggc gcctatgaga tcttccacga
     2221 gggaatttgc ataccgggat tgaaaatcgt tgagaagaac gaattccgcg aggacgtatt
     2281 tcagacgctg acgggacagt gccgtgatcc gcagtacgtg ggcctggatt tgaaggcgat
     2341 gatcgcggga aataatatct gcgccaagcg atatctgggc ttggtggaaa aattcggcct
     2401 caagttcgtg caattggcgg gcgaaaaaat gattgccgat tccgagagta aggcccgcgc
     2461 gaaacttaga tcgatgccgg acggcacctg gatgacccgc caatacgtca cgaccttgga
     2521 tcgcaagacg cgcaaatcgg tggcgcaaca actctactgt accatgacca agaaacgcga
     2581 cgagttgctg ttcgacttta ccggcacgag cccgcaaacc gataccgacc acaattcgac
     2641 cttgcccagc accgtggctc acctggcgtt ggcgctgacc aatactctgt tctgggacgt
     2701 gccgtggagc gatggcaaga tgcgtccggt aaaggttcat gtgccggaag gatccattct
     2761 caactgcaaa taccccgcgg cctgcggcgc cggaccgcgc atcggcaacg tgctcgtatc
     2821 gacggtctgc gaggcggtcg caaagatgat ctatgcttcg ggccgctatg acgacgtcaa
     2881 cgcctcgacc acggggaact tggaattcgt cggcggtccc ggatacttct acggtggtca
     2941 cacgcgcgaa ggcatcgccg tggctcaagg actctacgat atccacggag cgggaatggg
     3001 cgccaggccg catggtgatg gtgtcaatac cggtgggcac atgaatattc catcggcggg
     3061 tatatcggac atcgagcgca tcgagatgca gtacccgttt ctttatttta cccgcgcgca
     3121 caacccggac ggcagcggct tcgggcagta ccgcggcggg ctgggcagct atcgtattta
     3181 tctgatctat ggctcgaaag attgcagcgt cgactacaaa ccgtatggcg gtgtcgctca
     3241 gggtggcttc ggcctcgccg ggggttatcc gaccggcatc ggcgcaaccc gggtgatgat
     3301 cgaagccggt ctcgaaatgc tcggccgagt caaagccggc gaatatccga cggtggcagc
     3361 gatgcgcgcc ggagaatggg gcaaatcaat cttaccggca ggcgtgcccg agcgagtgcc
     3421 attgccggaa ggcactttgt tggtcgacta cgtagccgga ggcggtggat ttggcgaccc
     3481 catcgatcga gaccccgccg ccgtcttgaa agacttcggt tacggctggg taagccgaga
     3541 aatcgcggaa agaatctacg gcgttgtctt tgccgacggc ggtaagcttg tcgacggcgc
     3601 cgccacggaa aactgccgcc gcgaaatacg cgaagcgcgc aggcaagacg cgccgatgtc
     3661 gcagaagtcc aacggtctgg cgggcgaggt tggccgtgac tcgcgcccac cgctgcgctt
     3721 tcacgcggct ttggagatcg ttggcaacgg caagagaaag actatccgtt gcagccgctg
     3781 cggtcacctg tactgcaaag gcgatgaaaa ctacaaacag catgcgcttc atcggattgt
     3841 ggatctcaat gacgtcatgc cgccattgcc cacgggcgaa cgttatatcg gcgaatacca
     3901 catctactca tgcccgggct gcgccacgca gctgcaagtg gatttgttta gcccgagcct
     3961 tggcggcgat ccggtgttgt gggatacgag aatagcatag ctcacatcca gtcaggaaag
     4021 atcgatatgg cattcggatc gaatgaaacg tgagcaacca tcattatgac atggtgaaat
     4081 ccgccggatg cgactcctgt tcatggacta agcatcatgg cgctttggag gcgacaaatg
     4141 aaattgagcc gattcgcgag gcttctcttt gcagtgattt tgctggcgtc agcggcgcaa
     4201 gaaataagcg cgcaggatcg tatcagtctc ggcatcacga ccaagaccgg cagtactggc
     4261 ctgccgttcg taatcgcgga agagaaagga ttttttaaga gcgaaggtct taacgcggtt
     4321 tcggtggtga tgcagaacca ggtcgtggtc aatggcgttg tcgcgcgcca ggtcgattac
     4381 ggcgggacct tctcgaactt cgttggagcg gctctgtcgg ggcttcccgt gcgcatcgtg
     4441 atggccgtca tggagggctc ggaccattac ctggtgacga gcccgaacat aaaaaaagtc
     4501 gaagacctca agggcaaaaa attcggcatc agcagtttcg gcggcactcc gcacagcgaa
     4561 gccgtcatga ttctccggaa gtacgggatg aatccggaaa aggatgttac gtttctgcaa
     4621 atcggcggca gttcgtcccg ttatacggca ttggatagcg gcgccatcga tgccgcgatg
     4681 ctggtgccgc ctttcaacaa gcttgccgct aagcggggat ttaacgaaat cctttctttc
     4741 aatgagatta tgaacatccc cttgggaggc ctgaccgttc acacccaaag gatcaaagaa
     4801 aaacccgatg aaatcgtcag aatgatcaag gccatgctga aaggggtcaa ttacatccgc
     4861 aacaaccaag ccgaaatcct ttcaatcatg gcaagcaaat ggggtatcaa agaggcggat
     4921 gtgcgcgagg gcatttaccg cgacatcgtc gggatctaca ctcgaaatgg cgtcgcctcg
     4981 gacgagacta tgaagaacgt tattcaactg gtgcgcgaca cgcgaaaatc caaggatgat
     5041 ttggggctgt ccgacatcgt cgactggagc tttgccaaaa aagcacaagc cgaattaaag
     5101 attcgatgac tcctgctcca ggttcaagtg gtttaaatag ttcaagccgt tcaagtcgtt
     5161 tcagatgaac tcgcggagac gggggtttga actgcttgaa cggttccagc gttttgaacc
     5221 atcggctcgt tctacgaacc tagcttcttg aagaatccct ctttttcgat atcatcgagc
     5281 agcgaatgat cgatgaactc ctccgcggtg cgatttttca attcgggatt atgctcggca
     5341 tagaatttga tcgtgtcccg cacaccggat aaatcgaccc gcggcggcag tgaaaaacgc
     5401 ggcgcgaaat agttataggt tgattggagc gtttccgcgt cgtcgatgcg catgcgtctg
     5461 gcaaacacct tcatagttct cgcgcggtca tttttgatcg cgtgaaccgc ttcggagtag
     5521 gcccggacga aacgcttgac ccgatcgcgg tcttcccgga gaaaactgcc tttgatgtag
     5581 agagaggatt gcgtgaagtt agcgctcatc tctcccatat cggctaatac ccgatagccc
     5641 gcctgaaccg ccttggttag atgcggcggt gaaagtatcg tggcgtccgt tcggccggtc
     5701 atcagcgatg cgaggcgagt cggcgcatcg ccgccgatga tcacctgaat atcaggcagc
     5761 ttcagacccc attcccttaa cgccagctga agggcgagat cattggagcc gccgaaattg
     5821 agaatgttga ctttcttacc gggtaaatcc ttgggtgtgc gaatctcggg cttgacgata
     5881 aaggcgtaag ggaatttgtt gtacgaagcg gcgatgactt ttaggtcggc gccgcgagca
     5941 gcggcgacga tagggccggt tgccgagcct gtggcaaact gggtgctgcc gcccagcaac
     6001 gccgccaccg agcgagcact gccgttgatc atgatcacat cgacatccag gccgtgctta
     6061 tcaaatattt ttagatcctt cccgacccac acagtgcttt gaaaaccgga gagcccgcca
     6121 tagtcggcga cgagcttgtc cgctgcttct ggcctggtga tcaacagcca tagtaacgac
     6181 aatgccgaaa acgcagcgga gatgagcttc atgttaactc ctgagtcgtc attccgggca
     6241 agctggggat agctagcgcg acccggaatc cgcttgtaag ggcgggtttg aaacccgccc
     6301 ctacagatgc ccgctttcgc gcgcatgacg gtgggagacg tcgattcttt tgcaaatgtc
     6361 caatccttag ccgtcagtta agtctcggca tgatctcttt tttgaagaga tcgagagaag
     6421 aaatgacttt ctgatgagcg aggttgccaa tgtggaacat acccatgaag tgattcgcgc
     6481 cgatctgctt catttgatcc tgaatttgcc gcgtaacgct gtcgggatcg cccgcgatca
     6541 tataaccgct ctttatcaac tcgtcccagc cgagcgttga gaaatcgatg cgcgaacgat
     6601 tgccttcacg gaaatagttg aaagagcgtt cgctggtgag tttggccagg agtttctgtt
     6661 gatcggccgc cttttgattg atggcgtcat tgaagcctcg attgtagacc gaaaaaaaat
     6721 accgcatagc cggctcggcg atctcttgag ctttcttgtt cgagtcatcg atatagatgt
     6781 gacggcatag gatgaaatcg tcgggcttgg cgttccaccc atcctccttg gccttgatac
     6841 gatagtagtt gaaggtatct tcgatctgcc cgttggtctg gtacacctgc gcgcatggca
     6901 ggtgccgctt aacgcaccac tcgatcgatt cggcgctgcg cgccgcgatc caaataggcg
     6961 gatggggttg ctggacaggg cgcggccaaa tggcgcaatc tttgagcttg taaaattctc
     7021 cctcgaagct ccagaccgaa ttggtccacg cggtcatgat cagatcatag gcttcttgaa
     7081 atcgcccgcg cgattcttcc ggattcacgc cgtaccatac atactcggcg ggaatgccgc
     7141 gcacgaaccc ggcgatcaac cggccattcg aaaggcagtc caccatcgcg tattcttcgg
     7201 caacgcgcac cgggtgatcg cgcagcggaa gaatatttcc gagcacgccg atcttgattc
     7261 gcttggttct ctggctcaaa gcagcggcga taagatttgg cgacggcatg gtgccgtagg
     7321 aactatagtg atgctcattg aaaacaacgc cgtccaggcc aagctcttca caataggcca
     7381 tctcgtccaa gtgttcggca tagttggcgg cgccttttat cggatcgaaa aaattgctcg
     7441 gcaccggcgt ccgcggcgcg tcttt
//
LOCUS       DASUVS010000302        14038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_121959, whole genome shotgun sequence.
ACCESSION   DASUVS010000302 DASUVS010000000
VERSION     DASUVS010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14038
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121959"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VF182_16270"
     CDS             <1..468
                     /locus_tag="VF182_16270"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6438695.1"
                     /translation="LGNIALHSGDMPEAEKWFSEAREIDAEHPAVLLAWGRLLAAQGK
                     AETDENRSKAQLQQAREFLEKSKIKGEDSATIHSELGDIYFRLSMWDKAAAAYEEALR
                     LRRRRNDWRRILGQAYAQLGRIRAAERKFREVLAFSPDDVEAWRGLQSIGKKY"
     gene            611..1420
                     /gene="proC"
                     /locus_tag="VF182_16275"
     CDS             611..1420
                     /gene="proC"
                     /locus_tag="VF182_16275"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX6438696.1"
                     /translation="MVKETIGIVGAGKIGSAIARGIIRAGLVTADQIMASDVSDALRQ
                     AIATELGIRVSPDNQQLCDFADVVILAVKPQILDSVVKQMATSLGAGKLLVSVAAGVP
                     LSRIEAGLAPGARVVRVMPNICCVVGAGAAAYAGGTYATPRDLEIVGAILNSFGVGLP
                     VEEKDLDAVTGLSGSGPAYVFLFIEALADGGVKVGLTREVALKLALHTVYGAARMALD
                     SAKHLSELKDEVTSPGGTTIAGLYALEQKSLKGIVMDAVVSAAKRSQELGG"
     gene            complement(1470..2051)
                     /locus_tag="VF182_16280"
     CDS             complement(1470..2051)
                     /locus_tag="VF182_16280"
                     /inference="COORDINATES: protein motif:HMM:NF018701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HEX6438697.1"
                     /translation="MRRRFTVFLLLLLSACVTVNIYFPAAAVQRAADEIVKETWGGPN
                     GKSEQVQPQSLLRFAPSRIVSLALTREAFAQEADINVSNPAIRGLRESIKRRSGAIKP
                     YLDKGNAGINQDGLLVVRSSDGLSLKERAEAQQLIEAENRDREALYAEIAKANNIPAE
                     AVPKIKGIFAKSWIDQAQSGWWIQDSQGSWKRK"
     gene            complement(2060..4849)
                     /locus_tag="VF182_16285"
     CDS             complement(2060..4849)
                     /locus_tag="VF182_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438698.1"
                     /translation="MERELSIGTGYEIAFNDISFGFPFRIIGTALTIGQSDHVVATAA
                     GIAISVNPLDLLHKKIQRVTIDRPMIHLELDEILKPPAQAFSPLRIRHLEIHDGAVIL
                     NAADHKGVEFSSINLRAENLTLGQLSSISVNADVPWLKAKAELFFTREKDETSVNLVL
                     REHSFDRLLPLPDPEKTAKELLRLQAKLTTTPNEKTNLTVSAKFDDFKIGFKPLTGLL
                     DARVTSNTNFSQATVTAKLEVANFHNLVTEASEGNEGAAVATLIGSYSAADQRFSVTA
                     LKISTPLGTAHAQGTVSTDSDVFLNATRLSVLNVPWQALKTFLPEPFNQWSYDGMAAA
                     ELTLNGSWRSLHVTGTVTSSKLNIQGSNFSLADVALHAPIDWKASTLELNKVSIKGQS
                     LVAGSKGRTGGGAEQVQIHGSVGYTTEAPLVVNGRVEISGGRFASADGTTVGENLLLS
                     GSIEVISETPGHSMRFAGKLSLQRGEMLWGKFFTDLGGQRPVLDFDLDYLPGGDSVRL
                     RQARLALASIGDIGVSGTINQLARAPLADIQAHAENFNAGGFFNFFVQENFKRQYPIL
                     DNLSVGGQIAFQLHAQGNLDHLVAAGRMTLENGELRGKPNDWEIAPVALTLPFQISYP
                     EKSNSANRATPGILSIRGGRFGSRSLEPITTAVSLSHNTLLLHQPVRLALFGGSVEIA
                     NLAWPDIINDPKAMSFSLELKLLQLQELTDALGWPRFSGTLSGAIPQIRSTRETLRSE
                     GQVHADLFGGQLQIDRMEIENPFAAIPSLKLTARFQNIRLEQVSDTFSFGKISGILDG
                     VVNDLVITDGQPARFQAQAETVERPGSSQWISVEALNKITILSSGQDSNVLYSGLAGF
                     FENFRYSKMGFRASLSNDKLILRGVESRDGKEFLVVGTLLPPTVNIISHTQEIGFSEL
                     LRRLERITTESPQVK"
     gene            5293..6015
                     /locus_tag="VF182_16290"
     CDS             5293..6015
                     /locus_tag="VF182_16290"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX6438699.1"
                     /translation="MQKNKYLALTAAGFFLAGCTGMTRNQAATVGALTCGVAGAGAGV
                     YAAHQGINGKHKNEAAGAAIGAVSGALLCGGLAYLIAEDPKPAPKPPPPPPPPPPPPP
                     PAPKPAPPPPPPPPPPPPPPPPAPKVERTIILDDVLFDFDRSNVKPEAAKILDRLVAF
                     MQENKNSKVTLSGHTDNVGSDAYNQKLSERRVAAVREYAVKKGVDAGRISGQGFGESK
                     PIADNKTREGRSKNRRVEIKVN"
     gene            6060..6443
                     /locus_tag="VF182_16295"
     CDS             6060..6443
                     /locus_tag="VF182_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438700.1"
                     /translation="MMVFFLASALFFPTLAPAENFTFKEIEARVLEYRNWLDQVGSNG
                     SRFWVRLDSSKRPHKLYVGEGFMRAGPDEKENFVEIFSRFLAGHPEKNMLIDIYDATS
                     GKPIGEYGFGGFKLFSLDVSSLPQK"
     gene            6527..7867
                     /locus_tag="VF182_16300"
     CDS             6527..7867
                     /locus_tag="VF182_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX6438701.1"
                     /translation="MKSVFALRQDAREIFNAALTAADSAKAVLRHLIFDGESVVIGRR
                     HYGLKDYRRIIVVGAGKGSALMCRAVEELIGEWLYGGIIIVKYGYAVPMKKIGIIEAG
                     HPIPDENGLIATQQVVSLLRETTTEDLVINLISGGGSALLWSPADGVSFREKQEMTRV
                     LLNCGAPIREVNAIRKHISKVKGGRLARLAYPSTLISLILSDVVGDSISAIASGPTAP
                     DPSTFSKCQIILDSYRLRTEKFGSIARLIDRGSAGEVEETPKPGDPIFEKVLNVVIGS
                     NRLAVSAAKEHAEILGYDVKVIEHLAEGEATELATAHAAIAKEIYHSGTARRPACVIS
                     GGEATVTVRGDGLGGRNQEFAVATALELAGSEGIVALIGATDGGDGPTDAAGGIVDGG
                     TVRRGKIKGLDARDYLNRNDSYHYLQATDDLLITGPTFTNVMDLRLFIIDTDLA"
     gene            8074..8289
                     /locus_tag="VF182_16305"
     CDS             8074..8289
                     /locus_tag="VF182_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438702.1"
                     /translation="MIDVSLRGVRCRAAQEILDSDGTVDVLTEGTIIYELEGEGGQLV
                     SVQWDTGTTCLVSSEEIEITDRSVSWQ"
     gene            complement(8359..10794)
                     /locus_tag="VF182_16310"
     CDS             complement(8359..10794)
                     /locus_tag="VF182_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HEX6438703.1"
                     /translation="MDRYICIHGHFYQPPRENAWLETVELQDSAYPYHDWNERITAEC
                     YAPNSVARILDDQGQIVQLVNNYSRISFNFGPTLLQWLENNEAEVYAAILSADRLSMQ
                     RFAGHGSAIVQPYNHMILPLASRRDRRTQVLWGIADFECRFKRRPEGLWLPETAVDLE
                     TLEILAEEGIRFTILAPHQASRMLRADAPQWQDVTGGKIDPTRAYSQQLASGKSISLF
                     FYDGPIARAVAFEGLLTRGDHFADRLLGAFSGQPSWPQLVHIATDGESYGHHHRFGDM
                     ALAYALNQIESKNLAQLTNYGEYLERHPPTQQVEIVEKSSWSCFHGIDRWWSNCGCST
                     GGQPNWNQEWRTPLRNALDWLRDSLSGPFETQARKFLKDPWAARDDYIDVVHNRAPEI
                     VERFFSRHAVGPLDANSTVIALKLLELQRHTMLMYTSCGWFFDDLSGIETVQIIQFAA
                     RVVQLSQELFGESPESQFVEKLSAAKSNLSDRGGGRDIYDRLVRTAIVDWERIGAHYA
                     VRSLFESYPDKTSIYCFRAERDDYQVFNAGTARLAVGRVKLTSAITRESSILSFGVLH
                     MGDHNVNGGVLEFMPDGVYQGLIGELVSPFTRADFGEVIRVMDRCFGESNYSLRSLFR
                     EEQRTVIDNILASALGQSETLYRQIYEQRAPVMRYLTDLHVPLPKAFTAAAELVLNGD
                     LRRALLGQEINDERVSALLESAKIAGVTLDAPTLEFAYRQNLERLANGVARTPTLIVL
                     RQLRDATSLLVKLPFPVDLWRVQNIVYALTQSIYPRMQKRRMEGDDTAEEWLTIFEDL
                     SQKLAIKLPAQPA"
     gene            complement(10917..12164)
                     /gene="glgC"
                     /locus_tag="VF182_16315"
     CDS             complement(10917..12164)
                     /gene="glgC"
                     /locus_tag="VF182_16315"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX6438704.1"
                     /translation="MYVLGVVLAGGRGERLFPLTQDRAKPAVPFGGRYRIIDFVLSNF
                     VNSGILSIYVLTQFKAQSLLEHLDRGWRSTDFLGEHFVTAVPAQMRMGFEWYQGTADS
                     VYQNLYLIERHNPRVVAIFGADHIYRMNIRQMIDEHQRKNADTTVAALPVRINEASAF
                     GVIEVDPDWRIIGFDEKPSRPKSIPGEPDYALVSMGNYLFNTDVLVPALVSDAKNNRS
                     VHDFGRNLLPDLILDQRVFAYDFRKNRVPVPFKGEEASYWRDLGTIEAYYEANMDLCS
                     VDPSFNLYNRSWPLRTVGSGDPPAKFVFDWYDRKGMALDSIVSEGTIVSGSLVRNCVV
                     GRNVRIHSYSEIEDSVIMDWVEIGRHCKIRNAIIDKSNVIPSGTQIGYDATHDKEHYF
                     VSETGIVVVARGERKSSWIMTNP"
     gene            complement(12185..13624)
                     /gene="glgA"
                     /locus_tag="VF182_16320"
     CDS             complement(12185..13624)
                     /gene="glgA"
                     /locus_tag="VF182_16320"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEX6438705.1"
                     /translation="MKIGAISSEISPFAKTGGLADVVGSLSTALEQLGHELCLIMPAY
                     RSTLQGGFALRNVSLDVSVPLSDRQETATILKTKLGRDVIVYLVRADRYFNREFLYGT
                     KQGNYADNLARFVFLTRNALEILRHERVDIIHCHDWQTALAPVFLRTQPLRYPELASA
                     KTVLTVHNIAFQGNFWRADWHYLNLDEYLFSPAYLEFYQNFSLLKGGLIFADKITTVS
                     PTHAAEIRTKEQGFGLEGVLQQRANDLIGILNGIDYNQWNPAVDPVIAKQYSVNDLDG
                     KRFCKDSLQRAMGLAIKPDVPLFGIISRLTSQKGIDLIEQIFSALIERDVQFVLLGTG
                     DSRYEDFFTNAAAQFPGKVGVRIGFDESLAHQIEAGADIFLMPSLFEPCGLNQMFSLK
                     YGTIPLVRAVGGLKDTVQNYNAERSEGTGFVFEAYEAQELLETIDRALKLFPDKKSWT
                     ALQRRAMSMDFSWDRSAKAYSRIYHELLA"
BASE COUNT         3359 a         3647 c         3708 g         3324 t
ORIGIN      
        1 ctgggtaata tcgcccttca ctcgggggat atgcccgaag ctgagaaatg gttttccgag
       61 gctcgagaaa tcgatgcaga gcatcctgcg gttctactag cctggggacg cttgctcgcg
      121 gcgcaaggca aggcggagac ggatgaaaac cgttccaaag cgcaacttca acaggcgcgc
      181 gaatttttgg agaaatcgaa aatcaaaggc gaggactcgg cgacgatcca ttccgagttg
      241 ggtgatattt attttaggct gtcgatgtgg gataaagccg ccgcagccta cgaggaagca
      301 ttgcggctgc ggcggagaag aaatgactgg cggcgaatat tgggccaggc ttatgcgcag
      361 cttggtcgga tcagagcggc agaacggaaa ttccgggaag tcttggcgtt cagcccggac
      421 gacgtggaag catggagagg cctgcaatca atcggcaaga agtattagat tcaggtttgc
      481 aggctggttc acacgaaggc ccacggcaaa tcgccatggg ccttcgtgtt ttcctttccg
      541 gtcgactttg cagttcctat cgcagcgctc tattttggcg atgatcgaaa gcccctgaag
      601 gagaaatcca atggtaaaag aaacaatcgg cattgtcggg gccggaaaaa tcggttcggc
      661 gatcgcgcga gggatcatcc gtgcgggtct cgtaactgcc gatcagatca tggccagcga
      721 tgtcagcgat gctttgcggc aggccatcgc caccgagttg gggatccgag tatctccgga
      781 caatcagcag ctgtgtgact ttgccgatgt cgtcattctt gcggttaagc cacagattct
      841 agactccgtg gtaaagcaga tggcaacaag tttgggtgcg ggcaagctgt tggtttccgt
      901 agcagcgggt gtgccgcttt cccggattga agccggcttg gcgcctggcg ctcgcgttgt
      961 tcgagtgatg ccgaacattt gctgcgtcgt tggcgccggt gcggcggctt atgcaggggg
     1021 aacgtacgcg acgccgcggg atttggagat cgttggtgcg attctcaaca gttttggggt
     1081 tggcttgccc gtcgaggaaa aggatttgga tgccgtcacc gggttgagcg gcagcgggcc
     1141 cgcctatgtt ttccttttca ttgaggcact ggccgacggc ggggtgaaag ttggcctaac
     1201 gcgggaggtg gcgcttaagc tcgcgcttca taccgtctat ggcgcggcgc gcatggcgct
     1261 cgacagcgcc aaacacctga gcgagctgaa agacgaggtg acttcgccgg gcggcactac
     1321 tattgcgggt ttgtatgccc ttgaacagaa atcccttaaa ggaatcgtga tggatgcggt
     1381 tgtcagtgca gccaaacgct cgcaggaact cgggggatga taagaactca aatcacatcg
     1441 gttaatacgg cagcagagta aatccgcggt tacttccttt tccaacttcc ttgcgagtcc
     1501 tggatccacc agccagactg cgcctggtcg atccagctct tggcaaatat tcccttgatc
     1561 ttcggcaccg cctctgcagg tatattgttg gctttcgcaa tctcagcata cagcgcttcg
     1621 cggtcgcggt tctcggcttc gattagctgt tgcgcttcag cccgctcttt gagacttaat
     1681 ccgtcgctgc tgcgaaccac cagcaggccg tcttgattga tgccggcgtt gcctttgtcc
     1741 aaatagggtt tgatggctcc gctgcggcgc tttatcgact ctctcagacc acgtattgcc
     1801 gggttcgata cgttgatatc ggcttcttgg gcaaaagcct cacgggtgag ggcgagggag
     1861 acgatcctgg agggagcgaa tcgcagcaaa ctctgaggct gaacttgctc cgatttcccg
     1921 ttcgggccgc cccatgtctc tttgactatt tcatcggcgg cgcgctggac cgcggcagcg
     1981 ggaaaataga tgttcacggt aacacaggca ctgagaagaa gtaataaaaa cacagtaaat
     2041 cgtcgccgca tcagtcctcc tacttcactt gcggactctc ggtcgtaatc cgctctaatc
     2101 ttcgcaacag ctcagagaag ccgatctctt gtgtgtggct gatgatattg accgtcggcg
     2161 gcagcagggt gccgaccacc agaaattctt tcccgtctcg actttcgacc cctcgcaaaa
     2221 tcaatttgtc gttgctcagg ctcgctctga agcccatttt gctgtaccgg aaattctcga
     2281 aaaatccggc taaaccgcta taaaggacat tcgaatcctg tccggaactc agaatagtga
     2341 ttttgttcaa agcttcgacg ctaatccatt gactacttcc cggccgctcg acggtttcag
     2401 cctgcgcttg aaagcgcgcc ggttgtccgt cagtgatcac gagatcgtta acaactccat
     2461 ctaaaatgcc cgagatcttg ccaaatgaaa aggtatcgga aacttgctcc aaccggatgt
     2521 tttgaaaacg tgcggtgagc ttgagcgatg gtatggcggc gaacggattc tcgatctcca
     2581 tccgatcaat ttgcagttgc ccgccgaaca agtccgcgtg cacctgaccc tcgctgcgca
     2641 gtgtctctcg cgtcgaacga atctgcggga tggctccact caatgttccg ctaaagcggg
     2701 gccagcccaa agcatcggtc aattcctgaa gttgcaaaag ttttagctcg agagaaaaag
     2761 acatcgcttt ggggtcgttg ataatatcgg gccaggctaa attcgcgatc tccacactgc
     2821 cgccgaaaag tgcgaggcga accggctggt gtaaaagcag tgtattatgc gacaacgaaa
     2881 ctgcggtggt tattggctca agcgagcgcg atccgaaacg gccgccccgg atcgagagaa
     2941 ttccgggggt agcgcggttc gcgctgtttg atttttctgg ataactgatc tggaacggta
     3001 aggtaagggc taccggagca atttcccagt cattcggttt gccgcgcagt tcgccgtttt
     3061 ccaaagtcat gcggcctgcg gcaaccaggt ggtctaaatt gccttgcgcg tgaagctgaa
     3121 aagcgatttg tccgccaacg cttaagttgt cgagtatggg atactgacgt ttgaaatttt
     3181 cctgaacaaa gaaattgaag aagccgccgg cattgaaatt ttcggcatgc gcctgaatgt
     3241 cggccaatgg tgcccgcgcc agttgattga tcgtgccgct aaccccgatg tcaccgatgg
     3301 aggcaagagc taaccgggcc tgacgtagtc gaacgctgtc gccacccggt aaatagtcga
     3361 ggtcaaagtc caacaccggc cgttgtccac ccagatcggt gaaaaacttg ccccataaca
     3421 tttcaccgcg ttgtaacgat aacttgccgg cgaaccgcat cgagtgcccg ggagtttctg
     3481 aaataacctc gatgctgccc gacaagagca aattttcacc gacggtagtg ccatcggctg
     3541 acgcgaagcg gccgccgctt atttcaactc gcccgttgac gactagcggc gcttccgttg
     3601 tgtagccgac cgacccatgg atctgcacct gctcggcgcc gcctccagtg cggccttttg
     3661 aaccggctac gagcgattgt cctttgatcg aaaccttatt taactccagc gttgaagcct
     3721 tccaatcaat cggtgcatgt aaggcgacat cggccaagga aaagttgctt ccttggatat
     3781 tcaatttact gctagtcact gtgccggtta cgtgcagcga tctccaggat ccgttgagcg
     3841 ttaattccgc tgcggccatt ccatcatagc tccactgatt gaaaggctcc ggcaaaaacg
     3901 tcttgagagc ctgccacggc acgttgagca ctgacaaccg agtagcgttg agaaagacgt
     3961 cgctgtccgt gctgacggta ccttgcgcgt gagccgtgcc cagcggggta cttattttta
     4021 gagcagtaac tgagaagcgc tgatcggccg ctgaataact gcctatgagg gttgccacag
     4081 ctgcgccctc attaccttcg cttgcttcag tgacgaggtt atgaaaattg gcgacctcta
     4141 actttgcggt cactgtcgcc tgactgaagt tcgtattcga agtcacccga gcatcaagga
     4201 ggccggtcaa cggtttgaaa ccgattttaa aatcatcgaa ttttgcgctt accgtcagat
     4261 tcgttttctc gttcggagtc gtcgtcagct ttgcctgcag acgcaacagt tctttcgctg
     4321 ttttttctgg atcgggtaga ggaagcagac gatcaaatga gtgttcgcga aggaccagat
     4381 tgacgctggt ttcatctttc tccctggtga aaaacagctc cgctttggcc tttagccacg
     4441 gcacgtcagc attcacgcta atactgctta attgtccgag agtcaggttt tcggcccgca
     4501 agttgatcga agaaaattca acgcccttat ggtccgctgc atttaaaata acggcgccat
     4561 catggatttc caggtgacgt attcgcagcg gtgaaaatgc ttgagctggg ggtttcagta
     4621 tttcgtctaa ctctagatga atcatcgggc ggtctatcgt cactcgctga attttcttgt
     4681 ggaggagatc aagaggattg acacttatcg cgataccggc agcggtagct acgacatgat
     4741 ccgattgccc gatggtgagc gcggtaccga ttattctaaa cgggaagcca aaactgatgt
     4801 cgttaaaagc aatttcatag ccggttccaa tcgaaagctc acgttccaac cacagctgaa
     4861 atcgtgaaga gtagaagact gcaaagagcg ttgcgtaggc gagtgttatg agagtgagac
     4921 agaccagagt tgacgtaaca aagtaacgtt tcaaaacagc cagaagatac acgctggtaa
     4981 taagacgggc aactaggtgt cgctcggaat caccgctgga gtatcgaaac tatggcggca
     5041 gatagcaaac ggcgaatttt agccccgaca gaacccatgg atggaattcc cgaccgcgtt
     5101 tcataaatac gttcttacta tgggttgtag ggaggcaagc ttcctccctc tagatctagt
     5161 tacctcgaaa ttcgtagttc cgtgataacc ttaaagatta catgaagttc gtaaatggtt
     5221 aaattttctt gacaagcaca aattcctata ctacgttagc cgaaattttg actacttttt
     5281 ggggaggcag agatgcaaaa aaataaatat ctagcgttga ctgcagccgg ctttttcttg
     5341 gctggttgca cggggatgac gcgtaatcag gctgccacgg tcggcgcatt gacatgcggc
     5401 gtcgcgggcg ctggcgccgg cgtgtatgcg gcgcatcaag gcattaacgg taaacataaa
     5461 aatgaggcag ccggtgccgc aattggcgcg gttagcggcg cactgctttg cggcggtctg
     5521 gcctatttga tcgccgagga tcccaaacca gcgccgaaac cgccaccgcc tccaccaccg
     5581 ccaccgccgc cgccaccacc ggcaccaaag ccagcgccgc cgccaccgcc tccaccaccg
     5641 ccgcctcctc cgcctcctcc accggcgccg aaggttgagc gaaccatcat tttggacgac
     5701 gttctttttg acttcgatcg gagcaatgtc aagcccgaag cggcgaagat tcttgatcgt
     5761 ctggtggcgt tcatgcagga gaataaaaat tctaaggtga cattgtcggg gcacaccgat
     5821 aacgttggtt cagatgccta caatcagaag ttatcagagc gccgggttgc cgcagtcagg
     5881 gaatacgctg taaagaaggg tgtcgacgca ggacgtattt cgggacaagg gtttggtgag
     5941 agcaaaccaa tcgccgacaa caaaacccga gagggccggt caaagaatcg ccgggtcgaa
     6001 atcaaagtca actagaatca acaactcgga tggaattctc aatgcggatg ccaggaaaga
     6061 tgatggtttt tttcctcgca tccgcattgt tttttccgac gcttgctcct gcagagaact
     6121 ttacattcaa agaaattgaa gctcgagttc tggaataccg gaattggttg gaccaagtcg
     6181 gatccaacgg ttcacgcttt tgggtccgat tagacagcag caaaagaccc cataagcttt
     6241 acgtgggcga aggtttcatg cgggcaggtc ccgacgaaaa agaaaatttt gtcgagatct
     6301 tctcgcgctt tctcgccggc catcctgaga agaacatgct gatcgacatt tacgatgcca
     6361 ccagtggtaa acccatcggt gagtatggtt ttggcggttt taagcttttt agtctcgacg
     6421 tttcatcgct accgcaaaaa tagcactttg cttctatagc tctcgttacc caaagcccgc
     6481 atatcaagcc tttcattcgc tcgtaccttt ctataagtac agcgtcatga aatccgtatt
     6541 cgccttgagg caggatgccc gagaaatatt caatgcggcg ctaacggctg cagattccgc
     6601 taaagccgtt ttacgtcatc tcatattcga cggcgagtct gttgttattg gccgtcggca
     6661 ttatggcttg aaggattatc gaagaataat agttgttggc gcgggtaaag gctcggctct
     6721 gatgtgtcgc gctgtcgagg aattgatcgg agagtggctg tatggcggaa tcatcatagt
     6781 taaatacggc tatgcggttc cgatgaagaa gatcgggatc atcgaagcgg gccaccctat
     6841 tcccgacgag aatggattaa tagccacaca acaagtagtt agcttattgc gtgagactac
     6901 gaccgaagat ttagtgataa atctgatctc cggcggcgga tcggccttgt tatggagtcc
     6961 ggcggacggt gtctcttttc gcgagaagca ggagatgaca cgagtcttgt tgaactgcgg
     7021 cgccccgatc cgcgaagtta atgcgatcag gaagcatatc tcaaaggtaa aaggcggtcg
     7081 cctcgccaga cttgcttatc cgtcaacgct gatttcgttg atcttgtcgg atgttgtggg
     7141 agactcgatt tcagccatcg cttcgggtcc cacagcaccg gatccaagca cgttctctaa
     7201 gtgccagatc attcttgata gctacagact ccggaccgag aaattcggtt ccatcgcgag
     7261 attgatcgat aggggcagcg caggagaggt cgaagaaact ccaaagccgg gcgatccgat
     7321 attcgaaaag gttctaaatg tagtcatcgg cagtaatcgc ctggcggtaa gcgccgcgaa
     7381 ggagcatgcc gaaattctgg ggtacgatgt caaggtgatc gaacatcttg ctgaaggtga
     7441 agcgactgaa ctcgctacgg ctcatgccgc gatagccaaa gaaatctatc attcagggac
     7501 cgcccggcga cccgcttgcg tcatctccgg gggtgaggca acagttaccg tccgtggcga
     7561 tggcttgggc ggtcgcaacc aggaatttgc ggtggcaacg gcgctggagc tcgccggttc
     7621 tgagggcatc gttgcactga tcggagcaac cgacggcggt gatggcccca ccgatgcggc
     7681 aggagggatc gtcgatggcg gcacggttcg ccggggcaaa attaagggtc tcgatgcgcg
     7741 agactacctc aacagaaatg actcctacca ttatctgcag gcaacggatg atttgttaat
     7801 taccggaccc acctttacga atgtcatgga tctccggctg tttatcatag ataccgatct
     7861 ggcctgatta aaatcacgcg gttcaccgtc gtggcggtgt ttcaccagcc gagagtcaga
     7921 cttgctatag ccagccttaa tggatccaag cgagaaacaa gtttgcaaaa tgagttgatt
     7981 tcaattctgt tcatgattaa agtgcccgct tccttggtga tccggttttt cggacgagga
     8041 cggcgactta aaaaatttgc cgggagttga aaaatgatcg atgttagttt gcgaggagtt
     8101 agatgccggg cagcgcagga aattcttgat agcgatggca ccgtcgatgt cctcactgag
     8161 ggaactatca tttatgaact cgaaggtgaa ggtggacagc tcgtcagtgt ccaatgggac
     8221 accggtacga cttgcttagt atcatcggag gagatcgaga taaccgaccg cagcgtaagt
     8281 tggcagtgat cgcagcttgt catttaagcc gcaagcgtca gttcttcgcg tgtctcgctt
     8341 agatactcga aagctaattt atgccggctg agccggtaat ttgatagcca gcttttgcga
     8401 aagatcttca aagatcgtta gccattcttc tgctgtgtca tcgccttcca tccggcgctt
     8461 ttgcatcctc ggataaattg attgcgtcaa ggcataaaca atgttctgca ctcgccataa
     8521 atcgacggga aacggcagct ttaccagtaa gctagttgcg tcccgaagtt gccgcaggac
     8581 gatcagggtt ggagtccgtg ccacaccatt ggcgagccgt tccaaatttt gccggtaagc
     8641 aaattctaga gtaggtgcat ccagcgtaac tccggcgatt ttcgccgact ccaataaggc
     8701 actgacacgt tcgtcattga tttcctggcc tagaagcgcc cggcgcaaat cgccattaag
     8761 aaccagttct gcggccgctg taaacgcttt tggtagcgga acatgcaggt ccgtcaggta
     8821 gcgcattacc ggcgcgcgtt gctcgtaaat ttgccggtat aatgtctcgc tctggcccaa
     8881 ggccgaagcc aaaatgttat cgattacggt tctctgttcc tcacgaaaaa gcgaccgcaa
     8941 agaataattt gattcaccaa agcaacgatc catgacccgg atcacttcgc cgaagtccgc
     9001 gcgagtgaac ggggatacca gttcaccgat tagaccctga tagactccat ccggcatgaa
     9061 ctctaaaacc ccaccattga cgttatggtc gcccatgtgc agcacgccaa agctcaggat
     9121 cgatgattcc cgagtgattg cgctggttaa ctttacacgg cctacggcta gcctggcggt
     9181 gccggcgttg aatacctgat aatcgtcacg ttctgcgcga aaacagtaga ttgaggtttt
     9241 gtccggatag ctttcaaaca gtgagcgcac cgcgtaatga gcgccgattc tctcccaatc
     9301 gacgattgcc gtgcgtacga gccggtcgta gatgtccctt ccaccaccgc ggtccgatag
     9361 attgctcttg gccgcggata atttctcgac aaactgagac tccggtgatt caccaaacaa
     9421 ctcctggctc aactgtacta cccgcgcggc aaactgaatg atttgcacgg tttcaatccc
     9481 cgacaagtca tcgaagaacc aaccacagct ggtatacatc agcatggtat ggcgctgaag
     9541 ctcaagcagt tttagtgcta ttactgtgga gttggcgtcg agtggaccga cagcatgacg
     9601 tgaaaagaag cgctcaacaa tctccggcgc ccggttgtga acgacatcta tataatcgtc
     9661 gcgcgctgcc caaggatctt ttaaaaactt gcgcgcttga gtttcgaaag gtcctgaaag
     9721 cgaatcccgc aaccaatcca gcgcattacg cagtggtgtt cgccactcct gattccagtt
     9781 cggttgtcct ccggtactgc agccacagtt gctccaccac cggtcaatgc catgaaaaca
     9841 actccaggag cttttttcga cgatttcaac ttgttgcgtt ggcgggtggc gctccaaata
     9901 ttccccgtag tttgtcagct gggccaagtt tttggattcg atttggttca gggcgtaagc
     9961 caaggccatg tcaccgaaac gatgatggtg cccgtagctc tcgccgtcgg ttgcgatatg
    10021 taccagctgt ggccaactcg gttgacccga aaatgcgccg aggagccgat ccgcaaaatg
    10081 atccccgcgc gtcagtaacc cttcaaaagc gacggcccgc gcgatcggtc cgtcgtaaaa
    10141 gaatagcgaa atgcttttac cggaagctag ctgttgtgag taagcccgcg tgggatcaat
    10201 ttttcctcca gtcacatcct gccattgcgg cgcgtccgcg cgcagcatcc gcgacgcctg
    10261 gtgtggcgca aggatcgtaa atcgaattcc ttcttccgcc aaaatctcca gcgtttccaa
    10321 atcaacggcc gtctccggca accatagccc ttcgggtcgg cgcttgaaac ggcactcgaa
    10381 atcagcaatg ccccagagaa cttgtgtccg tcgatctcgc cggctggcca gcggcaatat
    10441 catgtggttg tacggttgca caatcgccga cccatgcccc gcaaacctct gcatgctcag
    10501 acgatccgcg gataaaattg ctgcgtaaac ttccgcttcg ttattctcta accactgaag
    10561 cagggtcggg ccaaaattga agctgatcct ggagtagttg ttgaccagct gaacaatttg
    10621 accctgatcg tctaggatcc gcgctactga attgggcgca taacattcag ctgtgatgcg
    10681 ctcattccaa tcgtgatagg gatacgccga atcctgtaac tcgactgttt caagccaggc
    10741 attttctcgc ggtggctgat agaagtgccc gtgaatgcaa atgtaacggt ccatctagat
    10801 tgatccgatt aatcgatgaa tacgggagaa catcagcgaa acagttggcc cttgaaggaa
    10861 tgatccgtga aagaaaacgt gaacgaagtg ggattttcgg gacgaggcac gggcaatcaa
    10921 gggttggtca taatccagct gctcttgcgt tcacctcggg caactacgac aataccggtc
    10981 tccgagacaa aataatgctc cttgtcatgc gtggcgtcgt agccaatttg cgttcctgaa
    11041 gggatgacat tggatttgtc aataatagcg ttgcgtatct tgcagtggcg gccaatctcc
    11101 acccaatcca taatcaccga gtcttcgatt tcgctgtagc tgtggatcct gacgtttcta
    11161 ccgacgacac aatttcgtac caagctgcca ctgacgatgg ttccttccga aacaatagag
    11221 tctaacgcca ttcctttacg atcgtaccag tcgaacacaa acttggccgg cggatcgccg
    11281 gacccgacgg tacgcaatgg ccagctccga ttgtaaagat tgaacgatgg gtccacagag
    11341 cataaatcca tatttgcctc ataataagct tcaatggttc cgagatcacg ccagtaactg
    11401 gcttcctctc ccttgaacgg aacgggtacg cgattcttgc gaaagtcgta cgcaaaaact
    11461 ctttgatcca ggatgagatc cggaagcaag ttccgcccaa aatcgtgaac gctgcgattg
    11521 ttcttggcat cggatacgag agcgggaacc agcacgtcgg tattgaacaa atagttgccc
    11581 atcgaaacta gcgcataatc cggttcgcct gggatggatt tcggccggct cggcttttca
    11641 tcgaagccga taatgcgcca atccggatcc acttcaatga cgccgaaagc actggcctcg
    11701 ttgattctca ccgggagcgc ggcaacggta gtatcggcat tcttccgctg gtgctcgtcg
    11761 atcatctgcc ggatgttcat tcgataaata tgatcagcgc caaagatggc cacaaccctc
    11821 gggttgtgcc gttcgatcag atatagattt tggtagacgg agtctgcagt gccctggtac
    11881 cactcaaatc ccatacgcat ctgggccggt acggctgtaa cgaaatgttc ccccaagaag
    11941 tcagtgctgc gccaaccacg atcgaggtgc tcaagcagtg attgggcttt gaactgggtc
    12001 aaaacgtaaa tcgatagaat gcccgaattg acgaagttgg agagaacgaa gtcgatgatc
    12061 cgatatcttc caccgaaagg cacggcaggc ttggcccgat cttgagttaa aggaaaaagc
    12121 cgttcacctc taccaccggc tagcacaact cctaagacgt acactgatcg ccctcgtatc
    12181 cggcttaagc cagcaactcg tgatagatcc tgctatatgc tttcgcggaa cgatcccacg
    12241 aaaaatccat gctcatcgct cggcgctgta acgcagtcca ggatttctta tcgggaaaca
    12301 gcttcaaagc tcgatcaatc gtttccaaca gctcctgcgc ctcataggct tcaaacacaa
    12361 aaccggtgcc ctctgacctc tcggcgttgt aattctgtac cgtgtccttt aagccgccca
    12421 cagcgcgcac gagtggaatt gtaccatatt tcaaactaaa catctgattc aatccgcacg
    12481 gctcaaagag cgagggcatc aaaaagatgt cagcgccggc ttcgatctga tgcgccagcg
    12541 attcatcgaa tccgatgcgt actcctacct tgcccgggaa ttgcgccgct gcattagtaa
    12601 agaagtcttc atagcgacta tcaccagttc ccaagagtac gaattgaaca tctcgttcga
    12661 tcagcgcgga aaaaatctgt tcgattagat cgatgccctt ttgcgaggtc aaccgcgaaa
    12721 taatgccgaa caagggcacg tcgggtttga tcgccaggcc catcgctcgc tgcagactat
    12781 ccttgcagaa tcgcttaccg tcgagatcgt ttacgctata ctgcttggcg attactggat
    12841 cgacggcggg attccattga ttgtaatcaa tgccgtttaa gatgccgatg agatcgttcg
    12901 ctcgctgctg gagcacacct tcaagcccga atccctgctc cttcgtcctg atctcggcag
    12961 cgtgagtcgg actcacggtg gttatcttat cggcaaaaat gagacctccc ttcaaaagac
    13021 taaaattctg gtaaaactcg aggtaggcgg ggctgaacag atactcgtca agattcaaat
    13081 aatgccagtc cgcgcgccag aaatttccct ggaacgcgat gttgtgcacg gttaaaactg
    13141 ttttcgccga ggcgagttcc gggtaccgca atggttgcgt ccttaagaat accggcgcca
    13201 gcgccgtctg ccagtcatgg cagtgaatga tatccacacg ctcatggcgc aagatctcca
    13261 atgcgttacg ggttaaaaaa acaaatctgg caagattatc ggcataattg ccttgctttg
    13321 tgccgtacag aaattcgcga ttgaaataac ggtcggcgcg aaccaagtaa acaatcacgt
    13381 cacgtcccaa cttggttttc aggatggtag ccgtttcctg tcgatctgaa agcggaaccg
    13441 aaacgtccaa cgatacattc ctcaaagcga agccgccctg gagagtcgaa cgataagccg
    13501 gcatgatcaa gcaaagctcg tgtccgagtt gctccagtgc ggttgataaa cttccaacca
    13561 catcggccaa acctccggtc ttagcgaatg gagagatctc agatgatatt gcgccaattt
    13621 tcatatcatg tgtcgatgct acgggctcgt tgtagtgtca tcgcgcagta gccgcagtga
    13681 atgtctcttt atggtccact gaagacgcaa cttgtgactg ctattgctca atcttgtgga
    13741 ctcatgaaag ttctgtgccc gcaaatctac cgtgtcttga atctctttct cgcgcgataa
    13801 ctttttaagt ttgtttcact ggtttgttcc tgagttaaca atggtggtct ccgcgtgtta
    13861 tctatcgcaa ttgcggcatg ctcgtaaacc gaacctgatt cctttagcca gacggccaaa
    13921 gcgaagagtg gaagcagaag agaacgacag aattaaacgc cgccgcgcgc tctttgtaaa
    13981 tccggcgacc attgcagctc catactacgg gcataattga aagcacacgc cgtcacct
//
LOCUS       DASUVS010000303        12493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1870930, whole genome shotgun sequence.
ACCESSION   DASUVS010000303 DASUVS010000000
VERSION     DASUVS010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12493
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870930"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VF182_16325"
     CDS             complement(<1..996)
                     /locus_tag="VF182_16325"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6438706.1"
                     /translation="MTYSRNTRATAAQSLARFICDLSYAQIPPDAVWRCRELLLDQLG
                     CQLIGASMPWNQPVYQFVNRTKSGGPARVVKYGNEVPLDDAVLVNGTFAQGCELDDYY
                     DQGGGHPGAATVPVILALAQQQTVSGQELITAMVAGYEVGWRIGRALLPELMNRGYHA
                     QSTVGVFIAATAAGKILRLDPEQMAHALAIAGSHSGGTMEYDQSGGEVKRLHNGMACC
                     GGLRSALLAQVGLTGPPTIFEGERGILKVMSGTSNVEPIVKNLDAGNKDLALYHAAMK
                     RFPVNASQHSPIELLDSLVRKHSIDPRQVTKITVELNEGILLHGGTIYQPREVIEA"
     gene            complement(1020..1253)
                     /locus_tag="VF182_16330"
                     /pseudo
     CDS             complement(1020..1253)
                     /locus_tag="VF182_16330"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
     gene            1317..1634
                     /locus_tag="VF182_16335"
     CDS             1317..1634
                     /locus_tag="VF182_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438707.1"
                     /translation="MSRKLIADPQRISQHFLARDFLHTHTLAVTQLVQYHIFSGEIPQ
                     ALHQRQRVFLISNPSQPPRQDKKAEPKSIELSGGILDQRRDIITFKRRRSVLGGNPKL
                     RLI"
     gene            complement(2068..3984)
                     /locus_tag="VF182_16340"
     CDS             complement(2068..3984)
                     /locus_tag="VF182_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HEX6438708.1"
                     /translation="MRDIYDAIYNRWLNDPEDFWAEAAEAVHWDKKWDRVLDDSRPPF
                     YRWFPGGSINTCYNALDRHVESGRADQPALIYDSPVTATVQTFTYRELLDHVARFAGV
                     LSAQGVQKGDRVIIYMPMVPEAIVAMLACARIGAIHSVVFGGFASHELANRITDARPK
                     VIVSASCGIEVGRIIPYKPLLDKAIELAAAKPEHCIIVQRPQQKAAMIGGRDRDWAEE
                     MAQTKPVGCVSVDATDPLYILYTSGTTGMPKGIVRDNGGHLVALQWSMQHIYGVEPGE
                     VYWAASDVGWVVGHSYIVYAPLFNGNTTVLFEGKPVGTPDAGAFWRVVSQHGVKVLFT
                     APTAFRAIKREDPNGECMRKYDLSGFRTLFLAGERCDPDTLLWAQKQLGVPVIDHWWQ
                     TETGWPIGANCIGLGMLPVKPGSCTKAVPGYDVRVLGDDGGELTPGQIGSIAIKLPLP
                     PGSLPTLWNNDEGYRKAYLARFPGYYLTADAGYKDEDDYLWVMTRTDDIINVAGHRLS
                     TGALEEVLASHPDVAECAVLGVADQIKGEIPVGLIVLKAGVRRSDGDIVREAVQMVRE
                     RIGAVASFRVATIVKRLPKTRSGKILRGTIKKIADGVDYQVPPTIDDPLILDEITANL
                     NALGYPAGSARNLV"
     gene            4319..5389
                     /locus_tag="VF182_16345"
     CDS             4319..5389
                     /locus_tag="VF182_16345"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6438709.1"
                     /translation="MKPLNRRPAIALIILLAFGTGESFAQQTPTKRNFEAEINAALQA
                     AKTAAGFEFLGTLVRTCLLPQSGGENLIDVAPAYVTDPSKAPPRETWLAEPAKVFDNL
                     YFVGGKLHSSWALTTKDGIILIDTIYPYNSEELIIGGMKKVGLDPAQIKYVLISHAHG
                     DHIGGAEMLQQRYGARIVMAGPDWDWVEKYPNRYKTMAPKRDIVATDGMKITLGETAV
                     TIWLTPGHTPGTMSYTFTVLDQSKPVNVAYSGGTAFNFVNNTPDPGIKNFQTYIDSQK
                     HMGAKAAEMKASVLLSNHSEFDNAVNKNRMLAGRGNGPHPYEIGADWVQRYFQVMQGC
                     ARAAQLKLEQRAAGAGPPKGEN"
     gene            5678..6451
                     /locus_tag="VF182_16350"
     CDS             5678..6451
                     /locus_tag="VF182_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6438710.1"
                     /translation="MKRQNLKSQPFLVEVRLNPAKIPSWEEYPFNLPAVRHIESVRFH
                     PSVTFFVGENGTGKSTLLEAIATGMGFNPEGGSRNFRFATRMTHSPLHGAMRLSRSAR
                     RQRDGYFFRAESYYNVATEIDELDEQPSFGPPIKDSYGGRSLHEQSHGESFLALFANR
                     LGGEGLYIVDEPEAALSPTRQLSLLSMIHELVLRGSQFIIATHSPILMAYPKAKIYLL
                     TENALEEVDYTATEHFSLTRAFLNDHRAMLRRLLGQDVV"
     gene            complement(6943..8115)
                     /locus_tag="VF182_16355"
     CDS             complement(6943..8115)
                     /locus_tag="VF182_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEX6438711.1"
                     /translation="MAVIDAIVRLQDEMTRWRRDIHAHPELGFEENRTSDFVAEKLRD
                     LGLEVHRGIGKTGVVGVLRAGSARRSVGLRADMDALPIHETNTFAHRSTNDGRMHACG
                     HDGHTAMLLGAAKYLADTRNFDGVVNFIFQPAEEGIGGARAMIADGLFEKFPCDVVFG
                     MHNRPGLALGKFAVRGGPMMAGGGFFDVRITGKGSHGARPETGVDPVLVAAHIVIALQ
                     SIVSRNVRPIDAAVLSVTKINAGDAYNVIPQTAQLSGTVRAFTREVMSLIETGMRRVV
                     NGTAEAFGAAAEVDFRVIFAPTVNDAAQADFMAAVCAEIVGEANVNRNPSLIVASEDF
                     SFMLEKVPGCYINIGNGAGEDACEVHNPAYDFNDQALALGASVFARIVEKKLPKGG"
     gene            complement(8577..9611)
                     /locus_tag="VF182_16360"
     CDS             complement(8577..9611)
                     /locus_tag="VF182_16360"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6438712.1"
                     /translation="MCRKPKYLLASLIVGYVITGVVFAAGDEGFYKGKALRLIVAFAP
                     GGGFDTYSRAIGRHMSKHIPGNPSIVVENMTGAGGFIHANYMYRQAKPDGLTVGNNIG
                     GLILQQIMGAKGANFDGKEFEFIGAPSIDNPVCVLTKASGITSMDQWFAAKQPAKMGG
                     VGAGGTASDVPRAVQAALKLPIRVVEPYKGTAEIKLSAESGELDGGCWAWESMKTMWP
                     GALKQGDVKVILLVMPKKHPELPDVPLAIDYAKTDDARRILKYAVHDIATLTRIYFVP
                     PATPKDRVELLRKAFADTLNDPEFLADAKKSKLDIEPVAGEELQKLVQDLYKIDPTTL
                     ARLKEVLLPK"
     gene            10014..10187
                     /locus_tag="VF182_16365"
     CDS             10014..10187
                     /locus_tag="VF182_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438713.1"
                     /translation="MPPVPVAPPTKTAFDLHRKEFEKYLTHEICDTDTYFTPAYRKEQ
                     REKVQAFYQMAWT"
     gene            complement(10184..10390)
                     /locus_tag="VF182_16370"
     CDS             complement(10184..10390)
                     /locus_tag="VF182_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438714.1"
                     /translation="MPWPFEDLVLGLGNSFGEYLRVPDVIASDHVMSLTTTNVGTMTS
                     SRRVGRLSVTPADPKLKILRQVSG"
     gene            complement(10854..12437)
                     /locus_tag="VF182_16375"
     CDS             complement(10854..12437)
                     /locus_tag="VF182_16375"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HEX6438715.1"
                     /translation="MIRRFFLLAFFLVPIATVSRAPAPAAAAPAACTIETFAALNLPE
                     TTITLVESLPAGTNSAPVGNIALPICRVRGVIAPANLFEVWMPTADWNGKFQALGNGG
                     LAGSISFGDMRTAVSRNYAAASTDTGHSSNAAGDPWWTNAQQIKDYGYRSIHELTLKS
                     KAIINALYGADPDRSYFVGCSTGGRQAFMEAQRYPGDYDGIIAGAPVYRVIELRARHV
                     WTWQCNQADPTGAHAIPVSKLSAIFNAVVAACDRRDGLEDGQVDDPRRCRFNPETLLC
                     TDADNDSCLTAPQIETLKCMYAGPVNPRTRKQVYPGVPATSELDQGPSIGAVPNPQYT
                     TFFQNTVFEDTNYDFLTFNFDTDLKFALNKKFDGETLEFIHQAEDPDLTAFKKRGGKM
                     IIWHGFSDPLPSPVDTIDYYNSIRARSNHSRRDAEKTEEFVRLFLLPNVGHCGGPAAG
                     GPNQFDPLTALEQWIEEGIAPEEIIASRVTDGVVTRTRPICAYPQTAQYVGSGSIDDA
                     ANFVCRQPHDRDRPHDHGR"
BASE COUNT         2639 a         3666 c         3468 g         2720 t
ORIGIN      
        1 ggcttcgatg acttccctgg gctgataaat ggtgccgccg tgcagcagga ttccttcatt
       61 gagctcgaca gtgatcttcg taacctggcg cgggtcgatg ctgtgcttgc ggacaagact
      121 gtcgagcaac tcgatcggcg agtgctggct ggcattcacc gggaaacgct tcatcgccgc
      181 atgatagagc gcgagatctt tattgccggc gtcgaggttc ttgacgatcg gctcgacatt
      241 gctggtcccc gacatgactt tcagaatgcc gcgttcgcct tcgaagatcg tcggcggtcc
      301 agtgaggccg acctgcgcca gcaacgcgga gcggagcccg ccgcagcagg ccataccgtt
      361 gtgtaaccgt ttcacttcgc ccccgctctg gtcgtactcc atggtacccc cggaatgact
      421 gccggcgatc gccagcgcgt gagccatctg ctccggatcg aggcgaagaa ttttgcccgc
      481 ggctgtagcg gcgataaaaa cccccaccgt gctttgcgcg tgatagccgc ggttcatcaa
      541 ttccggcaag agcgctcggc cgatgcgcca gccgacttca taaccggcga ccatagctgt
      601 gatgagctcc tgcccgctga ccgtctgctg ctgcgcgagc gcgagaatca cgggcactgt
      661 tgccgcgccg ggatggccgc cgccctgatc gtagtaatcg tcgagctcgc aaccctgagc
      721 gaaggttcca ttgacaagca ccgcatcgtc gagcggaact tcgttgccat atttaacgac
      781 ccgggccggg ccgccgctct tcgttctgtt gacaaattga tagaccggct gattccacgg
      841 catgctcgcg ccgatcaact ggcaacccaa ttgatccaac aatagctccc ggcagcgcca
      901 gacagcgtcg ggcggaatct gcgcatagct tagatcacag atgaaccggg caaggctttg
      961 tgccgctgtc gctcgcgtat ttctagaata tgtcattgtc gcaactcgtc tgccaccggc
     1021 taccgcgccg ccggtacgga cctcgcgctg ccgctgccga gtttatgtga gatctcggcg
     1081 gcgactcgct ttacggcctc cagcagcttg cttcttcctt tcgcctgcag tctgaacggc
     1141 gtcaaggtag tcgtcaggga gtagggagcc cggccttcgc tatcgaagat cggcgcgctg
     1201 atgacgcaga gtccgtccat gacgcgcgcg ccgcagctat aggaatatcc ctttctcttt
     1261 gtccggccag atccccgcta atttttcgga tcggtgatcg tgcgcggggt cagcgcatga
     1321 gccggaaatt gatcgccgac ccgcagagaa tctcgcagca cttccttgcg cgcgactttc
     1381 tccatacaca cacgcttgca gtcacgcaac tcgtgcaata tcacattttc tccggtgaga
     1441 tcccgcaggc gcttcatcaa cggcaacgcg tcttcctgat ctctaatcct tcacagccac
     1501 cgcggcaaga taaaaaagcc gagcccaagt cgatagagct tagtggcggc atcctggatc
     1561 aacgccgcga tatcatcacc ttcaaaaggc gacgcagcgt gctcgggggg aatcctaagc
     1621 tgcgactgat ctgaaacagc gactgctcag gaccgtgggc ttcaaacgaa tagagcagat
     1681 cgatggattt ttctagaact cgcgcttcaa gcgactttgg tccatattat ggaaggccct
     1741 ttcatagctc tacaacgatc ttctattagg agcgtacact gtggtcaaga gttggatcgg
     1801 cgaaaggggg aaggcgggga gcgcgagtcg ccgccgtctc gttcggcaca aggacgaacc
     1861 ggtgtggaaa ttaccaacaa caacgcagca aacactaaac atatgcgaga gccaccggcg
     1921 cgatcttcac aaggtatgct agatcgacat ggaattcacg cagaatggcg caaacattcc
     1981 cggtgaaagc agaagattgg aggaagctcc atcaataacg actgaccgcg gcaattcttt
     2041 tcatgcaact tcacgccaac aagacgatca gaccaagttc cttgccgaac cggcaggata
     2101 gcccagcgcg tttaaattgg cggtaatttc atcgaggatc aacggatcat cgatggtggg
     2161 tgggacttgg taatcgacac cgtcggcaat ctttttgatg gtacctcgca gaatcttgcc
     2221 cgagcgcgtc ttcggtagcc gcttgacgat cgtagcgacc ctgaacgaag ccacggcgcc
     2281 gatcctttcg cgcaccatct ggaccgcctc tctgacgatg tcgccgtctg agcgtcgcac
     2341 gccggccttg agcacgatta gccccaccgg aatttctcct ttgatctgat cagcgactcc
     2401 caagaccgcg cactcggcga cgtcgggatg agacgccagc acctcttcca gtgcaccggt
     2461 agagagccga tgaccggcga cgttgatgat atcgtcggtg cgggtcatca cccaaagata
     2521 atcgtcctca tctttgtatc cggcgtcggc agtgagatag tagccgggaa acctggcgag
     2581 gtaagccttg cggtaacctt cgtcgttgtt ccacagtgtc ggcaaagaac cgggcggcag
     2641 cggcagcttg attgcgatgc tgccgatctg acccggcgtt aattccccgc cgtcatcgcc
     2701 caatacccgg acatcgtaac cgggcaccgc tttcgtgcac gaacccggtt ttaccggcag
     2761 catccccaat ccgatacaat tggcgccgat cggccagccg gtttcggtct gccaccagtg
     2821 atcgatcacc ggcacgccaa gctgcttttg cgcccacagg agcgtatccg gatcgcagcg
     2881 ctcgccggcg agaaagagcg tgcgaaaacc gctcaagtcg tacttgcgca tgcattcgcc
     2941 gttgggatct tcgcgcttga tcgcgcgaaa cgccgtgggc gccgtgaaca aaaccttcac
     3001 gccgtgttgt gagaccaccc gccagaacgc gccggcatcg ggagtgccga ccggtttgcc
     3061 ctcgaagagc accgtggtat tgccgttgaa tagcggcgcg taaacgatgt acgagtgacc
     3121 gactacccag cccacgtccg acgccgccca atagacttca cccggttcca caccatagat
     3181 gtgctgcatg ctccattgga gcgcgaccaa atgcccgcca ttgtcgcgca cgattccttt
     3241 gggcatgccg gtcgtgcccg aggtgtaaag aatgtaaagc ggatcggtgg catcgacgga
     3301 aacgcagccc actggttttg tttgcgccat ctcttctgcc caatcgcgat cgcgtccgcc
     3361 gatcatcgcc gctttttgtt gcgggcgttg gacaatgatg cagtgctccg gcttcgctgc
     3421 cgcaagttcg atcgccttgt cgagcaaagg tttgtacgga atgatccggc cgacttcgat
     3481 accacacgaa gccgaaacga tcaccttcgg tcgcgcatcc gtaatgcgat tggcgagctc
     3541 gtgggaagca aagccgccga agactacgga gtgaatcgcg ccgatgcgag cgcacgccag
     3601 catcgcgacg atcgcctccg gcaccatcgg catgtaaata atgacccgat cgcctttctg
     3661 aactccttgc gcgctaagca cgccggcaaa gcgcgccacg tggtcgagca actcgcgata
     3721 ggtgaacgtc tgaacagttg ccgtaacagg gctgtcgtag atgagcgccg gctgatccgc
     3781 ccggccgctt tcgacgtggc gatcgagcgc gttgtagcaa gtattgatcg atccgccggg
     3841 gaaccatcga tagaacggcg gccgcgagtc atcgagcacc ctgtcccact ttttatccca
     3901 atgcaccgct tcggccgcct ctgcccagaa atcctcagga tcgttcagcc agcggttata
     3961 gatggcgtca tagatatctc tcatggcgtc ccttctgaca ctagtggatg cgtttcttcc
     4021 gtcgatgatc gatctgtgat ccaccatatt gcggcgtgta gctcctgcgc aagtgttcgg
     4081 cggcgtgttg gcacgttgaa ctttcaacat tgagccgtgc actttgaaaa aacgccgaga
     4141 gggacaactt gatgcagcag ctgtttgtca gacaagagat tgaccatagg aaaacccgtt
     4201 tcaggaatgt cttacggcta gacctgccct aaattcctct gcagaataat tcgatagaca
     4261 aatggctggc cttgtctgat attagctcca ttgcgaatct tgtaatgcgg agggatcgat
     4321 gaagccactt aaccgaagac cggctatcgc tctgatcatt ttactcgcct tcgggaccgg
     4381 cgaatcattt gcgcaacaaa ctcctaccaa aagaaatttc gaagccgaaa tcaacgcggc
     4441 attgcaagcc gcgaagaccg ccgccggctt tgaatttctc ggtactctgg tcaggacctg
     4501 cttgctgccg cagagtggcg gcgaaaattt gatcgacgtt gcgccggcgt atgttactga
     4561 tcccagcaag gccccaccac gcgagacctg gcttgccgag ccggccaagg ttttcgataa
     4621 tctatatttc gtcggcggta agctgcactc ctcatgggcg ctgacaacta aggacggcat
     4681 tattttgatc gacacgatct atccgtacaa ttcggaggaa ttgatcatcg gcggcatgaa
     4741 gaaagtcgga ctcgacccgg cgcagatcaa atatgtgctc atctcgcacg cccacggcga
     4801 tcacatcggc ggcgccgaaa tgttgcagca gcgctacggc gcccgcatcg tcatggctgg
     4861 tccggattgg gactgggtgg aaaaatatcc gaaccgctac aaaacgatgg cacccaaacg
     4921 cgacatcgtc gcaactgacg gtatgaagat cacgctcggc gaaacggcgg ttaccatttg
     4981 gctcacgccc ggacacacgc ccggcacgat gtcctacacc ttcactgttc tcgaccaaag
     5041 caagccggtc aacgttgcat actccggcgg cacagccttt aatttcgtaa acaacacacc
     5101 cgaccctggc attaaaaatt ttcaaaccta catcgactcg caaaaacata tgggcgcaaa
     5161 agcggctgaa atgaaagcca gcgtgctgct ctccaaccac tccgagtttg acaacgccgt
     5221 caataagaac cggatgctcg ccggccgtgg aaatgggccg catccctatg agatcggcgc
     5281 cgattgggtg cagagatatt ttcaggtcat gcaaggctgt gcacgggcag cgcagttgaa
     5341 acttgaacag cgagcggccg gagcgggtcc gcccaaggga gaaaactgat tctgagatct
     5401 gccgtatttt taggtttagt tttatctcat gggacgcggc cgttagacga ctgacgtccg
     5461 ttcatcgatc gacgtatgcg caggtccgac tctgttgctg tgcggtaatg tgacacgatg
     5521 tcagggttaa gtgctggcga tgcgcccggc cgagcgctgt ataacaagta tttgagcaaa
     5581 ccgccggaac cgccgataga acggcgctgg agtcatcgag cacccggtct caggcttatc
     5641 cgatgagctg cttcggcagc atccgcagag tggtacgatg aagcgccaga atctgaagag
     5701 ccagccattc cttgtcgaag tccgtctgaa tccggccaag atcccatcct gggaggagta
     5761 cccgttcaat ctgcctgctg tccggcacat cgagtcggtc cggtttcatc cctctgtgac
     5821 gtttttcgtc ggggagaatg ggacgggaaa gtccacgctg ctcgaagcga tcgctacagg
     5881 catgggcttc aatcccgagg gcggctcgcg aaactttcga tttgccaccc gtatgacgca
     5941 ttcgccgctg catggggcga tgcgcctgag ccgatcagcc cgacgacaaa gggacggcta
     6001 cttcttccgc gctgaaagct actacaacgt ggcgacggag atcgatgaac tcgatgaaca
     6061 gccgagtttc ggaccgccga tcaaggattc gtacggcggc cgctctttac acgagcagtc
     6121 gcacggcgaa tcgtttctgg ctctatttgc aaatcgcctg ggcggtgagg gtttgtacat
     6181 cgtggacgag ccggaagcgg ctttgtcgcc aacccgacag ctcagcctgt tatcgatgat
     6241 tcacgagtta gtgctgagag gatcgcaatt cataatcgcc actcattctc cgatcctcat
     6301 ggcatatccc aaagcgaaga tttatctcct gacggagaat gcgctcgaag aagtcgacta
     6361 tacagccacg gagcacttca gcctgacgcg tgcttttctc aatgatcaca gagcgatgct
     6421 tcggcggttg ttaggccagg atgtagtctg atctctgcgc cttaaccgca cgtctagcgt
     6481 ggctatcctg cggcataccg cgaggcggca acatctgcga atgaacaccg agccagcact
     6541 cgtccggctg ccgtgcgagg acgatcgtac cccggcccaa acggtcgaag ggcgttccgt
     6601 cttcgtgaaa tccggtgctc gtccgaggcg cgatgggcat ggcccgtagg gtgcggctcg
     6661 gagaagctgt cagtgctcgt tcctcgatat acattccttc cttggctcgg aggtatgaac
     6721 aaaacgcatc tggaagatcg attctattgt cacgttcaca tttacttaga ggacaactca
     6781 atcacacttt accctccaag gcatttccaa agatcgaact aaaatctttt ttgatccgtt
     6841 agcctccgtg cctcgccggg gtgggattcc ttgacgagcg aggaaacgaa attcagcgtt
     6901 cgattcgttc gcgtcgtttt tgccctggca ccagaaaagc cgctagccgc ccttcggtaa
     6961 ctttttctca acaatcctag cgaacacgct cgcgccgagc gcgagcgcct ggtcgttgaa
     7021 atcataagcc gggttgtgca cttcgcaggc atcctcgccc gcgccattgc cgatgttgat
     7081 ataacagccc gggacctttt ccagcatgaa agaaaaatcc tctgaagcca cgatcagcga
     7141 gggattgcgg ttcacgttgg cttcccccac gatctcggca cagacagccg ccatgaaatc
     7201 cgcctgcgcc gcatcgttga cggtgggcgc gaaaatcacg cggaagtcga cctccgccgc
     7261 ggcgccaaac gcctccgcgg ttccgttcac cacccggcgc atgccggttt cgatcagcga
     7321 catgacctcg cgggtgaacg cgcgcacggt tccggagagc tgtgcggtct gcggaatcac
     7381 gttgtaggcg tcgccggcat tgatcttggt cacggacaac accgccgcat cgatcggtcg
     7441 gacgttgcgc gagacgatgc tctgcagcgc gatgacgatg tgcgcagcca ccagcaccgg
     7501 atcgacccca gtctcgggcc gcgcgccgtg cgagcccttg ccggtaatac ggacgtcgaa
     7561 gaacccgccg cccgccatca ttgggccgcc gcgcaccgcg aatttgccga gcgccagccc
     7621 cggccggttg tgcatgccga acaccacgtc gcaggggaat ttctcgaaca gtccgtctgc
     7681 gatcatcgcg cgagccccgc cgataccttc ctcggctggt tggaagataa agttcactac
     7741 gccgtcgaaa ttgcgcgtgt ccgcgagata tttcgccgcg ccgagcagca tagcggtatg
     7801 gccgtcatgc ccgcaggcgt gcatgcgccc gtcgttcgtc gaacggtgag cgaaagtatt
     7861 cgtctcgtga atcggcagcg cgtccatgtc ggcgcgcaga ccgaccgagc gcctggcgct
     7921 gccggcacgc agcacgccca cgactccggt ctttccgatg ccgcggtgca cctccaaccc
     7981 taaatcgcgc agtttttcag cgacgaagtc cgaggtgcga ttttcctcga aaccgagttc
     8041 ggggtgggcg tggatgtcgc gccgccagcg ggtcatttcg tcctgcaggc gaacaatggc
     8101 gtcgatcacg gccatattct tgttggtctt aagatgttaa tcggcaaacg caggtgtccg
     8161 agaatcacat gttaccgcgt ccatagatta agagttcaaa tgtttaacta gcgagccgtt
     8221 tcaatcattc cactcgttct gtcgctccat tccaactgtt cagcggatcc ttgacgtttt
     8281 tagctccggc ttgtctttgt aggtctgacc caaataaatt cgttcgatgg ccgattcacc
     8341 cgggtgccga cgatacgcag accgattttc agtacagcac ccatgatgtt tcctttgaag
     8401 tgctttaaaa tcactatcga tctcattcgc gtaatacaaa tcggcgatgc cctcggttcc
     8461 tatattattt catcgccttc gcagcactca gatactggag gtcgacgaac cgttcagcgg
     8521 aggaatcggc gttcgcgcaa tgattgctgc agacaagttc atacctgttc ccgtcttcac
     8581 ttcggcaaca gcacctcttt tagcctcgct agcgttgtcg gatcgatttt gtagagatcc
     8641 tgcacaagct tttgcaattc ttcgccggcc actggttcga tgtccagttt cgacttcttc
     8701 gcatcggcaa gaaactctgg atcgttgagg gtgtcggcga aagctttgcg caataattct
     8761 acgcggtcct tcggcgttgc gggcggcacg aaatagatgc gcgtgagcgt ggcgatatca
     8821 tgaacggcgt acttcaaaat gcggcgcgca tcatcagttt tggcgtaatc gatagccagc
     8881 ggaacatcgg gaagctcggg atgttttttt ggcattacca agagaataac ctttacgtcc
     8941 ccttgtttca gcgctcccgg ccacatggtc ttcattgatt cccaagccca acaacctcca
     9001 tcgagctcgc cgctttcggc agacaatttg atttcagccg tgcctttata cggttcgacg
     9061 actcgtatcg gcagcttcaa tgccgcctgg acggccctgg gcacatcgga agccgtgccg
     9121 cccgcgccaa ctccgcccat cttcgccggc tgcttcgccg cgaaccattg atccatgctg
     9181 gtgatgccgc tggccttggt caacacgcaa accggattgt cgatggacgg cgccccaatg
     9241 aactcgaatt ctttgccgtc gaagttggct ccctttgctc ccataatctg ctgcagaatc
     9301 aagccgccga tgttattgcc gacggtaaga ccgtcgggct tggcctgccg gtacatataa
     9361 ttggcatgaa tgaagccacc cgccccggtc atgttctcaa cgacaatcga aggattgccc
     9421 ggaatgtgtt tgctcatgtg ccggccaatg gcccggctgt atgtgtcaaa acctccgccg
     9481 ggggcgaaag ctacgatcaa gcgaagcgct tttcctttat agaatccttc gtcgccagcc
     9541 gcaaaaacaa ctcccgtgat gacgtaaccg acaatcaagc tggccaggag gtatttcggt
     9601 ttgcggcaca taggttttcc tttctggtgc agtcggcgtt ttcttatccc actccaagtg
     9661 acataatttg ggcaaccggg tcacgcgcag ccatgtcgca cgaagcaggt gcttgccgat
     9721 tgcgctgcct tgtcgaatac cccgccactt gctacatacc gcaagtcaca attctaaatc
     9781 aaatcacacc gctgtcgcgc tctttccaag tcatcgtgca tgacccgagg gactgcgggc
     9841 gctccactca cttcaagagt gcccgtacca tccgtcagat ggccaatgac ctgccgcgct
     9901 tctcgagcgt ctgaatgtgc gctcggcgca tatcatcggg cattccctgg gttggtgtat
     9961 cggactgtcg ctcgctctca actttccggg caaagtgaga agcctgatcc tcggtgccgc
    10021 cggttccggt cgcgccgcct acgaaaaccg ccttcgatct tcatagaaag gagtttgaaa
    10081 aatatttgac gcacgagatc tgcgacacgg atacctattt cacgcccgcc tatcgaaaag
    10141 aacagcgtga aaaggtgcag gcgttttacc aaatggcatg gacctaaccg ctaacctgtc
    10201 tgagtatctt cagctttgga tcggccggcg taactgagag gcgacccacc cgtctcgacg
    10261 atgtcatggt gccgacgttg gtcgtggtca gtgacatgac atggtcggaa gcaatcacat
    10321 cgggcacgcg caagtactcg ccaaacgaat tccctaggcc gagtacaaga tcctcaaagg
    10381 gccatggcat gggtccttct ggcagacgac ggaagaaacc aatcaatgga ttttacaaca
    10441 gtgggtacgc aatcacccgt gagtggacgg tgatggagcg ttcattgcgg catgtcctcg
    10501 tgtttttcac ccaacttaga gattcgggtt gggacctggc aggtcgcctc aatcgggtat
    10561 ctctcatgac acgttttacc ttgctctttg cgcaagaaga gagttccctc ttttggaccc
    10621 gagacagacg cctctacaat gccgtcagga ccagctaaac tgggttcacg cgctggggcg
    10681 ttaggatgag catgaatcgg ttcagcgcat cgggctattc ggcaagttta tctcgcttgg
    10741 agattcgaag ttcggattat accgcgggtg tctcctgcca ttgactactt ttcccgagca
    10801 agtcccttat ggacgcgcct tcatcactag cgccaccgct ccgcgtccgg tgatcagcgt
    10861 ccgtgatcgt gtggacggtc gcgatcgtgc ggctgccggc agacgaagtt cgccgcatcg
    10921 tcgatgctgc cagacccgac atattgcgcc gtttgcggat aggcacagat cggacgcgtg
    10981 cgggtgacca cgccatcggt caccctggaa gcgatgattt cctcgggcgc gatgccctcc
    11041 tcgatccatt gctcaagcgc ggtcagcgga tcaaactgat tgggtcctcc cgcggcaggt
    11101 cctccgcaat gtccgacatt gggcaagaga aacagtcgga cgaattcctc ggtcttttcc
    11161 gcgtcgcgtc ggctatgatt cgagcgcgcg cgaatactgt tgtagtaatc gatggtgtcc
    11221 accgggcttg gcaatggatc ggagaagcca tgccagataa tcatcttgcc gccacgcttc
    11281 ttgaaggcgg ttaaatcagg gtcttccgcc tgatggatga actcgagtgt ctcgccatcg
    11341 aactttttgt tgagcgcaaa cttaaggtcg gtgtcaaaat tgaacgttaa aaaatcatag
    11401 ttggtatctt caaataccgt attctgaaag aacgtggtgt actgcggatt tggcactgct
    11461 ccgatcgatg gtccctgatc aagctcgctc gtagccggca ctccgggata gacctgtttc
    11521 cgcgtccttg ggttcaccgg tccggcatac atacatttga gcgtttcgat ttgcggggcg
    11581 gtgaggcacg aatcattgtc ggcatcggtg caaagcaagg tttcgggatt gaaccggcaa
    11641 cggcgcgggt cgtcgacctg gccatcctcg agaccgtccc gtcgatcgca cgcggccact
    11701 accgcgttga aaatcgcact cagcttgcta accgggatgg cgtgcgcgcc agttggatcc
    11761 gcttgattgc actgccaagt ccagacgtgc ctcgcgcgca gttcgatcac ccggtagaca
    11821 ggcgccccgg cgataatgcc atcgtaatca ccagggtagc gctgcgcctc catgaacgcc
    11881 tgccggccgc cagtcgaaca tccgacaaag taggaacgat cgggatcggc tccgtacaac
    11941 gcattgatga tcgctttgga tttcaacgtg agctcgtgaa tcgaccgata gccatagtct
    12001 ttgatctgct gggcattggt ccaccacgga tcgccagcag cgttggagct atgacctgtg
    12061 tccgtcgaag cggcggcgta gttgcgactg actgcggtgc gcatgtcccc gaagctgatg
    12121 gacccggcaa ggccgccgtt gccgagggcc tggaatttcc cgttccagtc tgccgtcggc
    12181 atccagacct cgaatagatt cgccggcgct ataactcccc tcacccgaca gatcggcaag
    12241 gcgatgttac cgaccggcgc tgagttcgtg ccggctggca gcgactccac cagcgtgatg
    12301 gttgtctcgg gcaggttgag ggccgcgaag gtttcgatgg tgcaggccgc gggcgcggct
    12361 gcggcgggcg ccggggcgcg ggagaccgtg gcgatcggga cgagaaagaa agcaaggagg
    12421 aaaaagcggc ggatcatacg gcacctccta cttttggtgg gatgtttgaa gttttattcg
    12481 gagaagctgg acg
//
LOCUS       DASUVS010000304         7408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1216832, whole genome shotgun sequence.
ACCESSION   DASUVS010000304 DASUVS010000000
VERSION     DASUVS010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216832"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..939)
                     /locus_tag="VF182_16380"
     CDS             complement(181..939)
                     /locus_tag="VF182_16380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438716.1"
                     /translation="MAERPQDEIEKLIADKLSDAGIKFVTQQAIAGLAADFIVYAPDG
                     RQFIVEAKNWDFPGLKAEASRQAQHYQDAAKADGAFIVIPSLKRGSPDKGVVTVDGLV
                     PALKVEFENSKTRAQPSESTKVSRIVFAAMPFAPQYEDVFFIAMRQAAEQVRAVCERV
                     DRREFQGSVVDEIQRLIRQSVAVIVDLSEAKPNVLYEAGYAHALNKPCIHICSTSTEK
                     LPFDVSHWKTTMYHPGQTHKLAKELAQRLKAVLD"
     gene            complement(949..1443)
                     /locus_tag="VF182_16385"
     CDS             complement(949..1443)
                     /locus_tag="VF182_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438717.1"
                     /translation="MNGLISTVMAGRQYTIAINLAPERLLYAGPVIPGAWNHLRDEEE
                     AGMLGGEILIDTGAFGAMIDVEVAERLRLPIRGTKEIHGIHGYGTVSCYQAKLILPAK
                     DSTGKDLPFIKTIECIGVPSLTEKNREHGVDVIGIIGREFLQNVRLEIDGATGQLKLL
                     VNDR"
     gene            complement(1487..2143)
                     /locus_tag="VF182_16390"
     CDS             complement(1487..2143)
                     /locus_tag="VF182_16390"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX6438718.1"
                     /translation="MIREAHIEKSCREGDREDVTIASSAGKLPRSFYEQATVDVAKQL
                     LGKYLVRNDPQGVTVGRIVETEAYIGPHDLACHAAKGRTARTEVMFGCAGHAYVYFVY
                     GVHYMLNLVTEAFNHPAAVLIRALEPTDGIELMETRRGTEKRRNLCSGPGKLCQAFAI
                     DRSLNAADLCGDIIYTEDRGEPIPRFLARPRIGVDYAGKWKDKPFRFLVRGSEFISRP
                     "
     gene            complement(2466..2894)
                     /locus_tag="VF182_16395"
     CDS             complement(2466..2894)
                     /locus_tag="VF182_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438719.1"
                     /translation="MPRPSTSPSRVKAEDEGFRRFLYLGDIIELPLSGIAVMESKLQN
                     QRDQVKRVVRATIRGTRFMKQNRGQTIQMLSDYLNITSSQSAKAFDASMNSFSDDGLI
                     SDTAVNMDVQLAKERLKITKEIPLSQLVDWSLVKEIKAEK"
     gene            complement(2860..3435)
                     /locus_tag="VF182_16400"
     CDS             complement(2860..3435)
                     /locus_tag="VF182_16400"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438720.1"
                     /translation="MKPAAKRVINLAPALCALAALLMAVPVLAVQRVTIGTPSRGLFE
                     FPTVVAIRKGFYQEEGFAVDRVQMQPLIAVKALMSGDVDYLLAWGSAVRAAITGVPIK
                     AVAGIASRPLHVLIARPGIRAPEDLKGKVIGVDSVAGTVDYLSRVALRHYGMEPDKDV
                     KIIVTGESPTRLAALRAGSIDATAIDIAFAG"
     gene            complement(3451..4275)
                     /locus_tag="VF182_16405"
     CDS             complement(3451..4275)
                     /locus_tag="VF182_16405"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6438721.1"
                     /translation="MASQRATGISPMTIRFGKSVSAVRALPKAKGKGPGVIILHERYG
                     IDQHTKDLTVKLAQAGFVGLAPDLFHRFEGDREALLRGEQRVDLTDDGALEDLNGAVE
                     YLKRSPNVDGTRIGIIGVCQTGRQPLLLAAKRGDIAGAVVLYGAIGGKEWLPNDLRPT
                     AVDDLVAQVNCPVLGIFGEADHIISVEDVTRFRNALEKATKNYHIRIYRDAPHGWLND
                     TMPGRYRKEPAKDAWNLMMSFLKKCFANGWDSDRISWTFESDFSKQYDFKKNVRME"
     gene            4340..4723
                     /locus_tag="VF182_16410"
     CDS             4340..4723
                     /locus_tag="VF182_16410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX6438722.1"
                     /translation="MTASSSATLRLRDHWQPLIFLIMEFKDRSTLIANLTTIGRKTGQ
                     PRTVELRLVYHSGSYYASSSKVQGKHWCQNMIENPAVEITIKGNKFACIARQVTDDKL
                     RRKILTLRDSPPLMERVVFEITPER"
     gene            complement(4884..5777)
                     /locus_tag="VF182_16415"
     CDS             complement(4884..5777)
                     /locus_tag="VF182_16415"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6438723.1"
                     /translation="MVITDLQTGMAYALLAGTLWGMGPLALKRALKHTSVGTATLTEQ
                     YLSVLLLGVLAAYHGELAHLDFTSRAFWAFFLAGAIGASFGKIFYYKGIDRVGASKAT
                     SVKNSSPILTTFLAVIFLGEEMSGWILAGVLLIVAGVAALTRTEKPSREGANRLEYFL
                     FPLLAAICFGINPIFKKIGINAVSLPTLGAFITQATALLVMLTFGRFFSVKARWEPMP
                     LKALGLVTLSSLTEALGSLFTFFALIHAPAVLVSPMWRISPLVTFALVRFTLRGIEVV
                     TLRDGLAAGLIVGGVFVLTRG"
     gene            complement(5796..6011)
                     /locus_tag="VF182_16420"
     CDS             complement(5796..6011)
                     /locus_tag="VF182_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438724.1"
                     /translation="MRTALIIGTVLALAMGLSACGKNDKGSAARDAEIQKTLQEGSQR
                     ERQMYEGMQKGIENLEKQSSQKPDKTQ"
     gene            6134..6967
                     /locus_tag="VF182_16425"
     CDS             6134..6967
                     /locus_tag="VF182_16425"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEX6438725.1"
                     /translation="MLRSFSLLILLSGLMLAATFTSAQAPAAKPKVSSTLAVRTPGAW
                     KPLDSGIEQRKMTLERSEPSYSFDLNLVRFGPNRVLPRIISSGQFQLKSADAKTLAQR
                     SGAVATINANYFDEKGKPLAFLKTATQEINRSVSKHALYTGVFGVQDSTPFIVHRDDF
                     QLTQAQEAIQSGPLLLKEGAAVQTIAGAGRYSRRAVIGIDKQHRLIVGVTDTVLGGLS
                     FAELQELFSRPEWQLETTELLNLDGGGSAQLYIKTNRHEEFISGTADVPVVIGFFKTG
                     N"
     gene            complement(6964..>7408)
                     /locus_tag="VF182_16430"
     CDS             complement(6964..>7408)
                     /locus_tag="VF182_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX6438726.1"
                     /translation="PVTFGAFAVITALTEKDRPKEEYGHFAGLGFKRPFLAMAMTLFM
                     LSLAGFPPLAGFTGKFHIFRSAIAAGYTDLAIIGVLNSLLSLIYYLRVIVMMYMSEGG
                     VQGKSLAESPSLYVAVGLATVATIYLGILPTRAMEWSRVAYSTLF"
BASE COUNT         1706 a         2100 c         1903 g         1699 t
ORIGIN      
        1 gtgcatccca gtcgaacatc aattatgtat atacaatata cagccttcgc gcgagaatag
       61 aaaagcgacc ccagtcgcag ccgcctgaac ttcggcacca ggttttccta gcgagtaagt
      121 tggagccgat gctgcgcgct ggaaaggacc gcacttcgag ttactcgccg gtggtctaat
      181 ttaatcgagc accgccttga gccgctgcgc cagctctttg gctaatttat gcgtctggcc
      241 cggatgatac atcgtcgtct tccaatggct gacatcgaaa gggagttttt cggtcgaggt
      301 tgaacagata tggatgcacg gcttgttcaa ggcatgggca tagccggctt catagaggac
      361 attgggcttg gcctcggaaa gatcgacgat gacggcaacc gattgacgaa tcagccgttg
      421 aatttcatcg acgacgctgc cttggaattc tcggcggtcg acccgttcgc acacggctcg
      481 cacctgctcg gcggcctgac gcatggcgat gaaaaataca tcctcatact gcggcgcaaa
      541 cggcatcgct gcaaaaacga tccggctgac tttggttgat tcggaaggct gcgcgcgtgt
      601 cttgctgttt tcgaactcca ctttgagcgc cggcaccaga ccgtcaacgg tgacgactcc
      661 cttgtcgggt gagcctcttt tgagagacgg aatcacgatg aaggcgccgt cagctttcgc
      721 tgcatcctgg taatgctgcg cttgccggct cgcttcggct ttaagtccgg ggaaatccca
      781 atttttcgct tcgacgataa actgccggcc atcgggagca taaacaatga aatcggcagc
      841 caatcccgcg atcgcctgct gggtgacaaa tttaatcccg gcatcgctaa gcttatcggc
      901 aatcagcttt tcaatttcgt cttgtggtcg ttccgccatg tggattatct atcgatcatt
      961 cacgagcagc ttgagttggc cggtcgcgcc gtcgatctcc aggcgcacat tttgtaaaaa
     1021 ctcgcggccg atgattccaa taacgtcgac gccgtgctct cggttcttct ctgtgagcga
     1081 aggcactccg atgcattcaa tagtttttat gaacggcaag tcttttccgg tcgaatcctt
     1141 tgccggaaga ataagcttcg cctgatagca tgaaaccgta ccgtagccgt gaattccatg
     1201 aatctctttt gtgccgcgta taggcaggcg caaacgttcc gccacctcga catcgatcat
     1261 cgcgccaaat gcgccggtgt cgatcaatat ttctccgcct aacattccgg cctcttcttc
     1321 gtctcgcaaa tggttccagg cgcccgggat caccggaccg gcgtacagca aacgctctgg
     1381 cgccaagttg attgcaatcg tgtactgccg gcctgccatc acagttgaga ttagcccatt
     1441 cactggccaa cgcgtcaaaa actcgaacgt gattttctcc ttaagatcac ggtcgggaaa
     1501 tgaattcact gccgcgaacc aagaagcgaa atggcttatc tttccatttg ccggcataat
     1561 cgaccccgat ccggggtctt gccaaaaatc taggaatcgg ctcccctcta tcttccgtgt
     1621 aaatgatatc gccgcaaaga tcggcggcgt ttagtgatcg gtcgatagca aatgcctggc
     1681 agagctttcc cggcccgcta cataaattgc ggcgcttctc ggtccctcgt ctagtctcca
     1741 tcaactctat tccatcagtc ggttccaatg cccggatcaa aaccgccgcg ggatgattga
     1801 acgcttcggt gaccaagttg agcatatagt gaacgccgta aacgaaataa acatacgcgt
     1861 gcccggcgca tccgaacata acttcggttc gtgcggtgcg tcctttcgcc gcatggcacg
     1921 cgagatcgtg gggtccaatg taggcttcgg tttcaacgat ccggccgacc gtcactccct
     1981 gcggatcgtt acgcacgaga tacttgccta acaactgctt ggctacatcc accgtcgctt
     2041 gttcatagaa agagcgcggc agtttgcctg ccgaacttgc gattgtaaca tcttcccgat
     2101 caccctcacg gcaagatttc tcaatgtgtg cttctcgaat catcctactc tcatccaacg
     2161 aatagttaga cacgcagcca atgattgatg ggaacactcg taagccgcgg ccggttaact
     2221 acctgaggct cgcaggtcgc tgaagtatgc tggttgtatg ctgctaaaag aatgcataga
     2281 cgaaactcgt gctcgtgttc tgaaacgtga tcgagacttc cgaacgaaca cgaacacgta
     2341 gcacgagcac gaggtcaaac gaggcgcgcc aattaccgcg tgaattacaa ggatgaaagc
     2401 ggaattcaat attcggcttt cataattcat ctttccaacg tctatggtct ttttcagcag
     2461 gctgcctatt tttccgcctt gatctccttg accaaactcc agtcgaccaa ctggctcaac
     2521 gggatttctt tggttatctt cagccgttcc ttggcgagtt gcacgtccat gttcactgct
     2581 gtgtcggata tgagtccatc atcgctgaat gagttcatcg atgcatcaaa agcttttgct
     2641 gattgtgatg acgtgatgtt cagataatcg gagagcatct gaatcgtctg gccgcggttc
     2701 tgtttcatga agcgtgtgcc ccggatcgtg gcgcgcacga cgcgtttgac ctgatcacgc
     2761 tgattctgca gcttgctctc cattacggca atccctgaaa gcggcagctc gataatatcg
     2821 cccaggtaca aaaaccgcct gaatccctcg tcctctgctt taacccgcga aggcgatgtc
     2881 gatggccgtg gcatcgatag aaccggcgcg cagcgccgcc agccgagtag ggctttcgcc
     2941 tgtgacgatg attttcacat ctttatccgg ctccatgccg taatgcctga gcgccacccg
     3001 tgagagatag tccacggttc cggccaccga atcaacacca ataactttac ctttcaaatc
     3061 ttccggcgcc ctgatgcccg gccgggcaat cagaacatgc agcgggcgcg aagcgattcc
     3121 cgcgaccgct ttgatcggca ctccggtgat ggcggcgcgc acagccgatc cccatgcgag
     3181 tagataatcc acatcgccgg acatcagggc tttgaccgcg atcaacggct gcatctgcac
     3241 cctgtccaca gcgaagcctt cctcttgata aaatcctttg cggattgcga ccacagtcgg
     3301 aaactcaaag aggccgcggc tcggcgtccc aatcgtgacc ctttgaacag ccagcaccgg
     3361 gacagccatc aatagcgcag caagcgcgca cagcgccgga gctaagttaa tgactctttt
     3421 tgctgccggc ttcatggtca atctctggct tcactccatt cggacgttct tcttaaaatc
     3481 gtactgcttt gaaaagtcgc tttcaaaagt ccatgaaatt cggtctgagt cccaaccgtt
     3541 ggcaaagcat tttttcaaaa aagacatcat caggttccag gcgtcttttg ccggttcttt
     3601 gcggtagcgt ccgggcatcg tatcgttcaa ccaaccgtgc ggagcgtctc gataaatccg
     3661 gatatgataa ttcttcgtcg ctttctccag ggcattgcga aatctcgtca catcctcgac
     3721 ggagataatg tgatcggctt cgccgaagat tcccaaaaca gggcaattga cctgcgccac
     3781 cagatcgtcc accgctgtgg gtctcagatc gttggggagc cattccttac cgccgattgc
     3841 gccgtagaga accaccgcac cggcgatgtc accgcgcttc gctgcaagca ggagcggctg
     3901 ccggccggtc tggcatacgc cgatgatgcc gatgcgcgtc ccatccacat tgggcgagcg
     3961 cttgagatat tccaccgcgc cgttcaaatc ctccagagcg ccatcgtcgg tgaggtcgac
     4021 cctttgctcg ccgcgaagga gagcctctcg gtctccctca aagcgatgga agagatcggg
     4081 ggccaagccg acgaaaccgg cctgggccag tttcaccgtc aggtccttgg tgtgttgatc
     4141 gatgccgtag cgttcatgga gaatgatcac tccaggccct ttacctttgg ccttcggcag
     4201 cgcgcgcacg gcgctgacac tcttgccgaa gcggatcgtc attggtgaaa ttcccgttgc
     4261 tcgttgcgat gccatggata cctcccatac gattaaaata aactgaagcc gagtaaatgc
     4321 cgcagcttcc catgcagtag tgacagcgtc aagctccgcc acgctgcgct tgcgcgatca
     4381 ctggcagccg cttatatttt tgataatgga gttcaaagat agatcgactt tgattgcgaa
     4441 tctgacaacg atcgggcgaa aaaccggcca gccgagaacc gtagagctac gtttggttta
     4501 tcacagcggc agctattacg cgtcgtcaag caaagtacag ggcaaacact ggtgccagaa
     4561 catgatcgaa aacccggcgg tggaaataac catcaagggc aacaaatttg cctgcatcgc
     4621 aaggcaagtc accgatgaca aactgcgccg gaagattctt acactgcgag actcaccgcc
     4681 gctcatggag cgagtggttt ttgaaattac tcccgaacgt tgactttgct tcaagcagtt
     4741 taagtcgttc agccgttcaa gtcgcttgga ccctcgcaga aacaagatcg aatccggtat
     4801 gctttagctc gcgttcagcg tctccaacgc attgaacgtg ttgaactgct tgaacgactt
     4861 gaacgacttg aactctcgta gtgttatccg cgagttaata caaacactcc gcccacgatg
     4921 agcccggccg ccaggccgtc gcgcaacgtc actacttcga tgcctcttaa ggtaaagcgc
     4981 accagagcaa aagtgactaa aggtgaaata cgccacatcg gcgaaaccag cactgccggc
     5041 gcgtgaatca atgcgaaaaa cgtgaacaat gatccgagag cttcagtcag gctggacagg
     5101 gtcaccaatc ccagtgcctt caatggcatg ggctcccatc ttgctttgac cgaaaaaaac
     5161 cggccgaagg tgagcatcac cagcagtgcc gtggcttggg taatgaaagc gccgagagtc
     5221 ggcaggctca ccgcgttgat gccgatcttc ttgaatatcg ggttgatgcc gaaacaaatc
     5281 gccgcgagaa gcggaaacaa aaaatattcc aggcgattgg cgccttctct tgagggtttc
     5341 tccgtacgag tgagagcggc aacgccggcg acaattaaaa ggaccccggc caaaatccat
     5401 ccagacatct cttcgccaag aaagatgacg gcaagaaagg tggtcagaat cggtgaactg
     5461 ttctttaccg aagttgcttt ggatgcgccg actcgatcga tgcccttgta atagaaaatc
     5521 ttgccgaagc ttgcgccaat ggcgccggca agaaagaacg cccaaaaagc gcggctggtg
     5581 aaatccaggt gagcgagttc gccgtggtag gccgccagca cgccgagcag caacaccgac
     5641 aggtattgct cggtcaaggt cgcggttccc acgctggtgt gcttcaaggc gcgcttcagt
     5701 gccagcggac ccatgcccca gagagtaccg gctaacagcg cataggccat gccggtttgc
     5761 aggtccgtga tcaccatcga aggcagccaa ccgggttact gggtcttgtc tggcttttgc
     5821 gacgattgct tttccagatt ctcgatccct ttctgcatgc cttcatacat ctgtctctct
     5881 cgttgcgacc cttcttgaag cgtcttttga atttccgcat cccgcgccgc gctgccctta
     5941 tcatttttgc cgcatgccga taatcccatt gcaagagcga gcaccgttcc gatgattaaa
     6001 gccgttcgca tattgttggt ccttcttccg tttattgggt agtcgagtta cttagcagaa
     6061 aaatttgcct cgctccaact cgatattctt tgctttattc actggaatcg ttctgttaaa
     6121 ctcggcgaga ctcatgcttc gctcgttttc cttgctgatc ctgctctcgg gactcatgct
     6181 ggctgcgact ttcacctcgg cacaggcacc ggcagccaag ccgaaggtga gttccaccct
     6241 ggcggtgcgc acgcccggtg cttggaaacc tcttgattcc ggcatcgaac agcgcaaaat
     6301 gacgctggag cgttctgaac cgagctattc atttgatctc aaccttgtgc gctttggccc
     6361 taaccgggtt ctgccccgga tcatcagcag cggccaattt caactgaaga gcgccgatgc
     6421 caaaaccctg gcccagcgaa gcggtgcagt tgccacaatc aacgccaact acttcgatga
     6481 gaaaggcaag cccctggcct tcctgaaaac ggcaactcag gagatcaatc gcagcgtctc
     6541 taaacatgct ctctataccg gcgtttttgg cgtgcaggac tcgacgccgt tcattgtgca
     6601 tcgtgatgat tttcaactca cgcaggctca ggaagcgatt cagagcgggc cgttgctgtt
     6661 gaaggagggg gccgcagtgc aaacaatcgc cggcgcgggc cgatacagcc ggcgcgcggt
     6721 aatcggcatc gacaagcagc accggcttat cgttggagta accgatacgg ttctcggcgg
     6781 cttgagtttt gccgaattac aagaactgtt ttcccggccg gaatggcaac tggagacgac
     6841 cgagttgttg aatttagacg gcggcggctc agcgcagctt tacattaaaa ccaatcgcca
     6901 tgaagaattc atcagcggca cggcagatgt accggtggtg atcggtttct tcaaaaccgg
     6961 caattagaaa agcgtggaat aggcaacccg gctccattcc atagcccggg tcggtaaaat
     7021 cccgagatag atcgttgcta ccgttgccag ccccacggcg acatagaggg agggagactc
     7081 ggcgagagat tttccctgta ccccgccctc gctcatgtac atcatcacga taacccgcaa
     7141 ataatagatc agcgacagca aactattgag cactccgata atcgcgagat cggtgtagcc
     7201 cgcggcaatg gcggaacgaa aaatgtgaaa ctttccggtg aatccagcga gtggcggaaa
     7261 gcccgcgagc gagagcatga acaatgtcat cgccatcgct aagaacggtc gcttgaaacc
     7321 tagacccgca aagtgtccgt actcttcttt cggccggtcc ttttcagtca atgcggtgat
     7381 cacggcaaac gcgccaaaag tcaccggc
//
LOCUS       DASUVS010000305        22423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1654831, whole genome shotgun sequence.
ACCESSION   DASUVS010000305 DASUVS010000000
VERSION     DASUVS010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 22423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22423
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654831"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VF182_16435"
     CDS             <1..188
                     /locus_tag="VF182_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438727.1"
                     /translation="QSYMDSNPLKGGTLKNLIAGCPDANRQRCGLPDGRLAEKVQLQG
                     PGKVEAEVYALLTSERV"
     gene            243..674
                     /locus_tag="VF182_16440"
     CDS             243..674
                     /locus_tag="VF182_16440"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6438728.1"
                     /translation="MIKITDEMKDRINKAFDEKKYCVWATTSSDGYPDISFRGSTHVF
                     DNEHIAFWDRSLGTSTTNLENNPHVCMLYYDSAARTGWRFYGTATVYKDGDLRQQIMK
                     RVVKGEIDKDPERKGYGVLVRIDKIRGYSGFNIVQEREPQR"
     gene            692..1705
                     /locus_tag="VF182_16445"
     CDS             692..1705
                     /locus_tag="VF182_16445"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438729.1"
                     /translation="MKLWSASAAEVPGAFRSFTAKICLFVLLALGGGISDAEALDKVR
                     IAVSNPNMPNLTVAMAQHNGFFTEENIEAEIIRMNPNIAVTALATGDVDYCQLFGGVV
                     SGAMAGLPIRIVAGYLDNWPMTLIAQPEFKNLKDLKGKTLGISSFGATPDLAARMMLK
                     QFGIDAEKEIKILALGSDAARLTALKQRVVDFIVISPPADTQMEKQGYNIMARAYELF
                     SFPYLGLGTHTRKIKEKPDEIRRTIKATIRANRFIRDNRDEAVRTLVGWGKVERDFAY
                     ASYDALRNLFNADGAVPEDGLKLVIDQARRSAKIAREIASVEVADLTFLRAAQSELGI
                     KAR"
     gene            1954..2226
                     /locus_tag="VF182_16450"
     CDS             1954..2226
                     /locus_tag="VF182_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX6438730.1"
                     /translation="MGLSYEDLIEPAIVRQVSKTVAYCRCTRTKNPPFCDGSHAATNI
                     TPHILEFAEPTTIAICRCWRSKDHPYCDGTHGRLVKPKERPQKQHD"
     gene            2385..2771
                     /locus_tag="VF182_16455"
     CDS             2385..2771
                     /locus_tag="VF182_16455"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6438731.1"
                     /translation="MVEMKNLVKEADDNPGVNHLSLWRTDLAYFWVINCEPKVQDDMH
                     YHENDDHIFMVIEGECTVRTPRKEFVLKQFDTVLLKSGESYQLCNSGTGRMLLLGAGN
                     AGVNGQPRTRVPKIPSHIPLREPIIA"
     gene            complement(2836..4086)
                     /locus_tag="VF182_16460"
     CDS             complement(2836..4086)
                     /locus_tag="VF182_16460"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6438732.1"
                     /translation="MQSLWKYRTLRSLRHRNFRLLLEGALISSSGDFMQNVAQSWLVW
                     ELTRSPVALGIIAFFDTLPRLLVGAVGGAIADRFDRRRVLQVTQALAMTQAIIYWLAV
                     EFQFIEFWHIAGLAFFLGVVNTINQTARQSLVNSLVPKEELLNAIGLQSSVFNFSKIL
                     GPSIGGVIIAYIGVAGCFLINALSFVALLFNLYLMELPPWEARSNEQGIWSDVTEGFL
                     YLRGNRRILYIVGLSYVVAMFGAPYNRFVPMFATNILRVGPSGFGLLMSAPGVGATFA
                     ALSLASVRKLRVGTRAVCLCALGFAVSLALFAFSHSFVLSFIFLAMVGFFQIANRTLS
                     NTMIQIVTPSKLLGRVLSLFFMDRGLWSLGSVMIGAAAAVIGIDWTFAVSGAVCAIAA
                     LSLLHFSRRGRVPPGAAATVASPV"
     gene            4325..4840
                     /locus_tag="VF182_16465"
     CDS             4325..4840
                     /locus_tag="VF182_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydratase"
                     /protein_id="HEX6438733.1"
                     /translation="MFQMPIVNLLEESRHLLESGQRVRVLWQHPDTLVFIARGREYRS
                     EFHINPSDETMYMIKGEMCLYYRTPEGKEEVAVIREGAMIYTPAGTPHSPRFGADAFA
                     LISERKRREKETDRFHWYCQKCDALLHEEVFTVKDYAEDPVSKAYARFFGSKEFRTCN
                     CGEVMAAPESV"
     gene            complement(4924..5913)
                     /locus_tag="VF182_16470"
     CDS             complement(4924..5913)
                     /locus_tag="VF182_16470"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016869935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6438734.1"
                     /translation="MQYRYLGQQRLKVSAIGYGCPTFQGKLGDSDERRDIDVLHRAID
                     LGINFIDTADHNSGNNEELLAKALKGKRDQVVLASKFGNLRGQPWADGRDVNASPEYV
                     AIACEASLSRLQLETIDLYYLHRVDPQVPIEDTIGAMVRLIEQGKIRHIGLSEAGPDT
                     IRRANALHPITALQSEYSLWTRDYENDTLPVVKELGIGFVSYYALGRGFFAGAVKSLS
                     DLDPKDSRRKAPRFYEKNLQYNAQLLSRLQQIADDKRCTLAQLSLAWILHQGNNFVPI
                     PGTHRITHLEENVAAAEIVLESDEIAEIDRLFPRQGVASGARHDYDRSKELNI"
     gene            6072..7106
                     /locus_tag="VF182_16475"
     CDS             6072..7106
                     /locus_tag="VF182_16475"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6438735.1"
                     /translation="MIYRHVLWTSILAMWFLLFTPAKSAIAQESFYQGKTVRFIVGGS
                     AGGGYDTYTRLIARHLGKHVTGNPNFVVENMTGAGTLISANHVYKVAKPDGLTIGHFI
                     GGLLLQQVLGKPGIEFDGQKFEYLGVPAQDNTVIGISKASGVTSIDNWLATKTTLKFG
                     GVGPGSATDDIAKVVRATVGVPMQLVSGYKGTADIRLAIDSGELQGVANSWESFKSGW
                     AQEIQSGSVIIVLQMIPERRHPDLPNVPMISDIVKSEEARKIIQAGIYDYSAIARPYV
                     FPPNTPKDRVLMLRKGLLDTYKDPEFIADAQKARLDMSPLSGEELEKVVARTYKLEKP
                     IIEKLKEILK"
     gene            7161..8177
                     /locus_tag="VF182_16480"
     CDS             7161..8177
                     /locus_tag="VF182_16480"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438736.1"
                     /translation="MKNFPIIDADGHVTESLESLRKHLRKEHQARPLFTSEAWDRSFG
                     GALGKRNEDPKVQLADMDLDGIDIQIVFPTHLSLNSEKETALAIDIARAYNDWLAHFC
                     SVEPKRLKGVAMVALQDVNAAIKEVRRAVEDLGLVGVMMPTNVRDQDIGRREYWPFYE
                     EVERLDIGLALHGGIRAAERMHGRFDNFIAVHSLSFPFECMAAVTGLIFAGVPEKFPK
                     LRIAALEACCGWVPFLMDRLDEEFEKRGAKEAPLLKRKPSEYMSGEQFFYAFELEEST
                     VPYVIDRIGPGKLLYSSDYPHWDSSWPKTVRMFQEREDISEANKRQIAWENPQNFYGF
                     KAAS"
     gene            complement(8360..9826)
                     /locus_tag="VF182_16485"
     CDS             complement(8360..9826)
                     /locus_tag="VF182_16485"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6438737.1"
                     /translation="MFDSSKIQPHIVFTDLREWMREAEKLGELKTVLGASWQKDIGLA
                     TDVVVPPDDGPAVVFDEVPGCPRGFRILINAFAGKRRAMTFGFPQGLSKQELSQAYFD
                     HFQKAPNHMPPVFVKDGAIFENVLKGGDVDIMKFPTPVWHVNDGGRYIGTGCYSVTMD
                     PDEKWVNVGCYRAMIQDAKTVSLLMVPGKHGYMHREKYFKRGQKMPLALVLGGDPLFF
                     FMAGTEHPYGLCEYDIVGGMRGKPVEVVRGPLTGLPFPANAEIVFEGFLNNDNRKNEG
                     PFGEWTGYYASDESAQPVLEIEAVYHRNDPIILGVPPIGGGSDEMARYRAIMRSAMLK
                     QQLQSAGVPDVRQVWAHEIGASRMLIALAIQQRYPGHAKQVGALAASCGASVYGCKMV
                     IVVDDDIDVSNLDQLMWAMLSRYDPATSVDILRRMRSTPADPRLTPEQRKKRDFTNSR
                     MVIDATRPYDWKDKFPMVNAPSQEVVRMAREKFGYLLK"
     gene            complement(10017..10844)
                     /locus_tag="VF182_16490"
     CDS             complement(10017..10844)
                     /locus_tag="VF182_16490"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6438738.1"
                     /translation="MPYAQAGEVRLYYESFGRGVPFLFVSGTGWPGEPWKLKQVPAFA
                     DRYQVIVYDHRGVGKSDAPAGYYSTRQFAQDAANLLDALGINEPAHIIGHSMGGRVCQ
                     WLAIDYPRKVRSMIQAASGSGSMGNPDYPRWLTVDSLKSMIEKGYREHMLSHFKSRFF
                     FPPEFVKAHPEVLEMLFKSFWENRPQLDPYLRHVVARNQHETGELLEKIATPTLVLVG
                     GEDKADADTGSHVITSEHLRDNIHNAEFAVVEGCGHGFFWHKPEETNKIIGDWLDRH"
     gene            11211..12221
                     /locus_tag="VF182_16495"
     CDS             11211..12221
                     /locus_tag="VF182_16495"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6438739.1"
                     /translation="MKILSVVVALMLSSTGFAFAQESFFKGKTVRIVVPFAAGGGYDI
                     YSRIMGRHMGKHIPGNPVFVVENMTGAGGLIGMNHVYKAAKPDGLTLGTPIGTMFVDQ
                     LIGKPGIEFDGRKFEYIGAPAQDTQLLIVHKRTGIKTVEQWLSHATPVKFGSTGPGSA
                     NESIPKIVKEALNLPLQVVSGYKGSSVIRLAFTGGEVDGVANAWESMVSTWPQEIESG
                     AAVILLQASGKRHPDLPNVPLVMDHAKTDMAKKLVRAVINNFGATARPYVVTPGTPKT
                     RVEVLRKAFMDTMKDPEFGDELKKAKLDLNPLDGATLENNIKELFAVDQALIPKLTEI
                     LK"
     gene            12278..13345
                     /locus_tag="VF182_16500"
     CDS             12278..13345
                     /locus_tag="VF182_16500"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438740.1"
                     /translation="MIVDADAHVVETEHTWDYLDASEKKFRPQLFQSADNPNAQYWFV
                     DGKSIGFRPPTLTEQELIALSKSTGRELKTAPEARELRDVELRLKHMDKLGIDVQILF
                     NTMWIARVADKAEAEIALCKSWNHWMADVWEQGKNRLRWSCVVPAMTINEAIQQMRFA
                     KENGAVAVSLRPFEDDRHLVDPYFYPIYEEATRLDFAVAVHIANASPQLLDQFKPRYD
                     KMGGFAQFRIPTVITCLSLMMSEVPQQFPKLRWAFVEASAQWVPWVVKEAMRRTGTKG
                     FPDLPFKEFRIYVTTETNDDFDFVLRYAGEDNLVIGTDYGHTDASSEVDAIDIFRANG
                     SVTETVKKKILDDNPRALYTL"
     gene            13390..14760
                     /locus_tag="VF182_16505"
     CDS             13390..14760
                     /locus_tag="VF182_16505"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6438741.1"
                     /translation="MPKDLRSFIAELDASSPEDVARVTEPVSPRYEMSAILTHLERRK
                     RFPLLFFQNSKESQAPVVINAQASRRLMSIALECKPEDLARAFSERQSKPLAPVEMIN
                     APVHEISRTGDEIDLKQVPLLTHYDVNVAPYITAGIVVAVDPDTGVRNASYNRLMMAG
                     NRELRIFMAVGRHLWTLHNKLEGRNQPLPIAIVIGVHPLFSLGAQALTPTDEDEYAVI
                     GGMMNEPLRLVRAKTVPILVPADAEMIIEGQIMPNLRRTEGPFGEFTGHAVPQDQRQV
                     VEISAITHRENYIFQDIHAGYTEHKLMGAVPREAALLKAVRLSVPTVRNVCMPVSGNC
                     RFHAYISIAKRTPGQAKNAICAAFAADMLLKHVVVVDDDIDVFDEQRVLWAISNRFQA
                     DRGLVVIANAQGSELDPSAGPGGVNAKMGLDATKPLSGFPPELRVPEDILEKITLDDF
                     LPNFAK"
     gene            14950..15960
                     /locus_tag="VF182_16510"
     CDS             14950..15960
                     /locus_tag="VF182_16510"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX6438742.1"
                     /translation="MANIEQDKLRLLKGAVQDNVDLLRGIRTNTRRRQSKITRSWIWS
                     PLLLAVLAFFWQPSPVISTKSAAKPQDTKPNLKEAPAMTQSAAAEVQAPTTPLHPLDR
                     SVIPLGINRIVIDAGHGGKQPGAISESGVFEKDITLDIALRLHRLLETAPFEVLMTRH
                     DDRTITLDKRVAFANSNGADLFISIHINWTEPREIRPLETYFVGPSEDPATMKLASLE
                     NRESGYSLSEYRRVLEKIYIDARRDESRSLAKNIHAELYQSLKAVNPVLENRGVRTAP
                     FLVLVGTEMPAILVEVSTLSNEEEVELLIDPDYREKIALAVSKGINSYANALTRSAEK
                     GS"
     gene            15960..16997
                     /locus_tag="VF182_16515"
     CDS             15960..16997
                     /locus_tag="VF182_16515"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX6438743.1"
                     /translation="MSMEETNEPLCVGIDLGTSRSSISASNGERHVIDSYVGWPLDMV
                     ARKLIQKPLLVGREALDSRSMLDLRRPMERGLIKDGSKRDEEAVQEILHHLITQIGLK
                     QKNKTRSKVRAVVGVPAEAFRNSKQRLRSAMEGVADSVMLVSEPFAVAYGLEALLHAL
                     IIDIGAGTADFCVMQGRYPTEEDQRTLPNAGDSIDEQLLKLVREHHPEAQTSIHMIRE
                     WKEKWSFVGEAKQPVVVSVPVKGKATELNITAEMRAACESILAPVCETMLDLLTRVEP
                     EYQSKVRNNVILAGGTSLIVGLSQALEKALAELGGGHATVVEDPVFAGSDGGLAIARD
                     ASSSDWEKLSL"
     gene            complement(17102..17848)
                     /locus_tag="VF182_16520"
     CDS             complement(17102..17848)
                     /locus_tag="VF182_16520"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX6438744.1"
                     /translation="MRLQNQIAVVTGAGRNIGEDVSKLFAREGAKVAVVDLDAGRGGR
                     VASEINATRPGSAISVVCDVASAASVDQMVQTVVKQFGAIDILVNNAAWTDHKTLFDI
                     TEEEWDRVMNVCLRSVWYCTRSAAKVMIDHKRKGKIINIASTSGHWGRKEATAYTTAK
                     AGVLNLTRSIAVQLAPEGIRVNSVSPNRIGSPVGQEEVPENRFVKNLAGRRGVPMDIA
                     KAVVFLASDESDFIYAIDLPVDGGALAIRD"
     gene            complement(17950..18972)
                     /locus_tag="VF182_16525"
     CDS             complement(17950..18972)
                     /locus_tag="VF182_16525"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438745.1"
                     /translation="MSNRKSFHIRAALLLLVFSSVIIAGGLAFAADGAKKLRLAYAGW
                     ELGTAVAYVGVDAGLFKKYDIDIEEIPIRDNMSAGIQALLGVDLLIGSGSPLAILQPM
                     ANGADLAVIGSHVRLDQYGMGVAPSIGTVKDLKGKKVAVSALGARSDLIARVILRRAG
                     LDPVRDVEMVAAGLAPARALAISKNLVQGAPLSEEVAAEARKLGIKTLEVKAVPVVTD
                     LLVTTRSFIKRDEETIRRFMKGYAAAINHLVTRREESLAIIKKYFPDTQAGSVQAMYD
                     SFSSQLRPLPELNPESLQALVDVGAVIDKRTGSIKPSDVIEPRFLEELKNSKFLKDLY
                     VEKVSL"
     gene            19291..20115
                     /locus_tag="VF182_16530"
     CDS             19291..20115
                     /locus_tag="VF182_16530"
                     /inference="COORDINATES: protein motif:HMM:NF033683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEX6438746.1"
                     /translation="MATMITNECINCGACEPECPNNAISQGETIFVIDPQLCTECVGF
                     HDFEACAAVCPVDCCVTDPNNIETEDALIARARALHPETVIGDNFESRFRKGEGKSGA
                     PNGPKSAPAAASAAAAIAAAPLTTASAPQTSGYAEPTDNFIALPPIDAWEIPLRCFKC
                     SQTHPEPVRNFMIGNVIFCPHCHKSMVVRDNLNYHIRTLLTDSYEKWQKEQEAYINRR
                     ETELAQFMEKRAKDAQGFDAHQQRQLEHIRGQLAALGENYDAPGKPIKKGARFGWG"
     gene            20137..20610
                     /locus_tag="VF182_16535"
     CDS             20137..20610
                     /locus_tag="VF182_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438747.1"
                     /translation="MFNSWLHIIALVAYLGAILGLGLMLLPSLSSVEKHEDKVHFLAR
                     GLKFYNPLQVGALGLVLFSGAFELTHLKAMYREMFLKELGYNLAIKLTFAFLLVFFSV
                     YQAMGIGHRFVKRRELGESITPAELDSVVRRLTGANWLILFLAIITIWLGLRLQS"
     gene            20710..20841
                     /locus_tag="VF182_16540"
     CDS             20710..20841
                     /locus_tag="VF182_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438748.1"
                     /translation="MGNRQEMFVDIPKELGGLIAAIVDWCTTFQVDYLELRQLLRGG"
     gene            complement(20896..21645)
                     /locus_tag="VF182_16545"
     CDS             complement(20896..21645)
                     /locus_tag="VF182_16545"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX6438749.1"
                     /translation="MRVNTILSAGALGSLLLSQGCIATRDWVKEQMDPVSNRVSQSEN
                     RLTQGEGRLTQSEKQIGAIDTRITGIEGKLGQFEGRLGQVDAKANKALNALSNLRLER
                     RFVIDMKEGANFAFGSSNLPNQAQKEIDGFLSDLKGDPSGAGNTVFVVAGHTDNVGSD
                     DVNYELAKRRADAVSRYLITQKKMDPLKVMSVSYGESAPLAENNNAQNRAKNRRVEIL
                     VYREAITSEAVAATPQANSPAPQSETLSKRQ"
     gene            complement(21914..22297)
                     /locus_tag="VF182_16550"
     CDS             complement(21914..22297)
                     /locus_tag="VF182_16550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6438750.1"
                     /translation="MTAPYKVLVVDDDENFGQIMAETLIAAGYEVQTADDGLHGYLRY
                     LDQQPEIVLTDIQMPELDGLEMIRCIRTINPKVKAIYISGAISQYQEALIDEWKHHGA
                     LVLNKPFSRKDLLASMNGCLSDHPS"
BASE COUNT         5432 a         6097 c         5907 g         4987 t
ORIGIN      
        1 ggcaaagtta tatggattca aaccctctta aaggcggcac tttgaaaaat ctgattgcag
       61 gctgtccgga cgcaaacaga caaagatgcg gtttgcctga cggtagacta gctgaaaagg
      121 ttcagctgca aggcccggga aaagtcgagg ccgaggtata cgcgcttcta acgtcggagc
      181 gtgtctagca agtgcaacga acgagatgag cctttttcac cagccttcca ggaggaatca
      241 caatgattaa gataaccgat gaaatgaagg accggatcaa caaagccttc gatgaaaaaa
      301 aatactgcgt ctgggccacc acatcgagcg atgggtatcc ggatatcagt ttccgaggca
      361 gcactcatgt attcgataac gagcacatcg cattctggga ccggtcgttg ggtaccagca
      421 ccaccaatct cgaaaacaat cctcatgtat gcatgctcta ctatgactcc gccgcgcgca
      481 ccggctggcg tttttatggc accgcgacag tatataagga tggtgatttg cgacagcaga
      541 tcatgaagcg tgtcgtgaaa ggcgagatcg acaaggatcc cgagcgcaaa ggctatggcg
      601 tgctcgttcg catcgacaag atccgcggct atagcggctt caacatcgtt caagaacgtg
      661 aaccgcaacg atgagagcgg aaagcggaag gatgaagctt tggtcagcca gcgcggctga
      721 agtgccgggt gcctttcgat ctttcacggc caagatatgt ctttttgttc tgctcgcctt
      781 gggcggcggc atttcagatg cagaagctct ggacaaagtg cgtatcgcgg tgtcaaaccc
      841 gaacatgccg aacttgacgg tcgccatggc gcagcacaac ggtttcttca cggaggaaaa
      901 cattgaggct gaaattatca ggatgaatcc caatatcgcg gtgacggcgc ttgccaccgg
      961 cgatgtcgac tattgccagc tgttcggagg ggtcgtgagc ggggccatgg cgggtttgcc
     1021 gatcagaatc gttgccggtt acctcgacaa ttggccaatg acgctgattg cccagccgga
     1081 gttcaaaaac cttaaagatt taaagggcaa gactctcggc atcagcagct tcggggcaac
     1141 gccggatctt gcggcgcgca tgatgctcaa gcagttcggc atcgatgcgg aaaaagaaat
     1201 caagattctc gcattaggat cggacgcagc cagacttacc gcattgaagc aacgggtggt
     1261 ggatttcatc gtaatctccc cgcctgccga cacccaaatg gaaaaacagg gctacaacat
     1321 tatggcacga gcttacgagc tttttagttt tccatacttg gggctcggca cgcacaccag
     1381 aaagatcaaa gagaagcccg atgagattcg gcggactatc aaggcaacca ttcgcgccaa
     1441 tcgctttatc agggacaatc gcgacgaagc ggtgcgaact ctggtcgggt ggggcaaagt
     1501 ggagcgtgac ttcgcctatg cctcgtatga tgctttgcgt aatctcttca atgccgatgg
     1561 cgcggtgccg gaggacgggc tcaagctggt catcgaccaa gcgcgacgca gcgcaaaaat
     1621 cgctcgtgaa atcgcatccg tggaggtcgc tgacctgact ttcttgcgtg cggcgcaaag
     1681 cgagcttggg atcaaggcac gctagctcgg cacagcagag cttaggatgt ttaaaattgg
     1741 actcgctcgg tggaaagctg aacggatctg ccaagctact gcgtcatcag gtaagcgcga
     1801 catcgaagga ttatgcgcaa gggcaagaat attttggtca aaccgctgta gaaccgttca
     1861 agctcctaac cgcaaatccg cccgccggag tttgacaaca ctgcacggca gagaatattg
     1921 ttcattccct gcagcatttg gaaaggagaa aatatgggcc tatcctacga ggatctcatc
     1981 gagccggcaa tcgtcaggca agtatccaag accgtggcct attgccgctg cacccggacc
     2041 aagaacccgc ccttctgcga cggttctcat gccgcaacaa acataacacc gcacattttg
     2101 gaattcgcgg agccaacgac tattgccatc tgtcgctgct ggagatcaaa ggatcatccg
     2161 tattgcgacg gcacacacgg ccggctggtg aaacccaaag aacgcccaca aaagcagcac
     2221 gattagaaat aatgttcaat caggctgatt aaaacgttcc agctacgagc gtgcgatcga
     2281 tgaggatggg cggagacgtg cgcggcttac gttggagcga aatcggaagc aatgagatag
     2341 atggcgcgtt ctcattgatt tagtatatcg acaggaggga cgatatggtc gagatgaaaa
     2401 atcttgttaa ggaagccgac gataacccgg gcgtcaatca cctgagccta tggcgaaccg
     2461 atctggctta cttctgggta atcaactgcg aacccaaagt tcaagatgac atgcactatc
     2521 atgaaaacga cgatcacatc ttcatggtga tcgaaggtga atgcacggtg cgcacaccgc
     2581 gtaaagagtt tgttctgaaa caattcgata cggtgctgtt gaaatcgggc gaatcttatc
     2641 aactctgcaa cagcgggacc ggcagaatgc ttctactggg agcaggcaat gccggcgtca
     2701 acggccaacc gcgcacgcgc gtgccaaaaa ttcccagcca tatcccgctg cgcgaaccca
     2761 tcatcgcata atctccgatt gcccggcggt gaagacgtga ccgggatcgc ccgggctgtt
     2821 ggcggaattt cttcgctaaa ccggagaagc gacagtcgcg gcggcgccag gcggcacacg
     2881 gcctctgcga gaaaaatgaa gcaggctcag cgcggcaatt gcgcaaaccg cgccggatac
     2941 agcaaaagtc caatcgatcc cgatgaccgc ggctgccgca ccgatcatga cgctgcccag
     3001 ggaccataaa cctcgatcca tgaaaaatag gctgagcact ctccccaata atttgctcgg
     3061 tgtgacgatt tgaatcatgg tattcgagag agtgcgattc gctatctgaa agaaacctac
     3121 catggccagg aaaatgaaag ataatacaaa agaatgtgaa aacgcgaaca atgccagaga
     3181 aaccgcaaaa cctaaggcgc ataaacagac tgcccgcgtc ccgacacgca atttcctgac
     3241 gctggcgagc gataatgccg cgaaagtcgc cccgaccccc ggcgccgaca ttaaaagacc
     3301 aaacccggac ggtccaacgc gcaatatatt agtcgcaaac atcggaacaa accggttgta
     3361 cggcgcgccg aacatcgcca ctacatagga gagaccaacg atgtacaaaa tcctccggtt
     3421 accccgcaga tacagaaaac cctctgtgac atcactccag atcccctgtt cgttgctccg
     3481 cgcttcccac ggcggtagct ccatgagata gagattgaac aaaagcgcaa caaagctgag
     3541 cgcattgatc agaaaacagc cggcaacgcc gatgtacgcg atgatcacgc ctccgatgga
     3601 gggccctaag attttagaga aattgaagac cgaagattgc aggccgatgg cattgagcaa
     3661 ttcttccttc ggcacgaggc tgttgaccaa cgactgccgc gcggtctggt tgatcgtatt
     3721 gaccactcct aaaaagaacg ccagaccggc gatgtgccaa aactcaatga attgaaactc
     3781 cactgcgagc cagtagataa tcgcctgcgt catcgccaac gcttgggtaa cttgcaggac
     3841 ccggcgacgg tcaaaacggt cggcaatcgc gccaccgaca gcgcccacga gcaaccgcgg
     3901 cagtgtgtcg aaaaacgcga tgataccgag agcgacgggc gacctagtga gctcccaaac
     3961 aagccagctt tgcgcgacat tctgcataaa atcgcccgaa ctggaaatca aagccccttc
     4021 cagtagaaga cgaaagtttc tatggcggag ggagcggagg gttcggtatt tccagaggct
     4081 ttgcacgggt gatgtttcgg gtcaagaagt atgaatcagt tttacgcgat cacgaagccg
     4141 ttgcaaaggc tcagggcaat aggtgaaact tgaaattggc aggagtccgc tatttttttg
     4201 cggctaagct cggttcgaag catttttgat gcgggatcgt ttgtaacatg tcgccaggtg
     4261 aaatttggag ttaacgtcaa cgatgaaagt caattatccc attgattcat gaaggaaggc
     4321 aattatgttc caaatgccaa tcgtcaattt acttgaggag agccggcacc tattggaaag
     4381 cggtcaacgt gttcgagtcc tatggcagca tccggatacc ttggttttta tcgcgcgcgg
     4441 gcgggagtac cgcagcgagt ttcacatcaa tccgagtgac gagacgatgt acatgatcaa
     4501 aggcgagatg tgcttatact accgtacgcc cgagggcaaa gaagaagtcg ccgtgattcg
     4561 cgaaggagca atgatctata ctccggcggg cacgccgcat tctccccggt ttggcgccga
     4621 tgccttcgcg ttgatttcag aacggaaaag gcgcgagaag gagaccgacc gttttcactg
     4681 gtactgccag aagtgcgatg ctctgctgca cgaagaagta ttcaccgtaa aggactatgc
     4741 cgaggacccg gtctccaaag catacgctcg attctttggc agcaaagaat ttagaacttg
     4801 caactgtggc gaggtcatgg cagcgccgga atcggtctga ttaacggaac gtgttcaagc
     4861 cgcgcctgat tgagctgaat gatttgagct gcttgaacgt ttagaacggc ttgaactcct
     4921 caattagatg ttcagctctt ttgagcgatc ataatcatga cgggcgccgc tcgccacgcc
     4981 ttgtcgcgga aacagcctgt cgatttcagc tatttcgtcg gactctaaga cgatctcggc
     5041 cgcagcaaca ttctcttcca aatgcgtgat ccggtgtgtt cctggaatag gcacaaagtt
     5101 attcccctga tgcagaatcc atgcgagcga tagctgtgcc agggtgcatc tcttatcatc
     5161 ggcaatttgc tgtaaacggc tgagcagctg agcattgtat tggagatttt tctcgtaaaa
     5221 ccgtggcgct ttacgccggc tatcttttgg gtctaagtca gacagacttt tcaccgcgcc
     5281 ggcgaaaaac ccgcggccaa gcgcgtagta cgaaacaaaa ccgatgccga gctccttaac
     5341 tactggaagc gtgtcattct cgtagtcgcg agtccacagc gaatactcgc tttgcaaggc
     5401 ggtaatcgga tgaagcgcgt ttgctcggcg gatggtgtcc ggtccggctt cgctcagccc
     5461 aatgtggcga atctttcctt gctcgattaa tcgaaccata gcgccgatgg tatcctcgat
     5521 cggaacctgc ggatcgacgc gatgcaaata gtacagatcg atggtttcca gctgcagccg
     5581 gctcaacgag gcttcacacg cgatggccac atattcggga ctggcattaa cgtcgcgccc
     5641 gtctgcccag ggctggccgc gcaaattacc gaactttgat gcaagcacga cttgatcgcg
     5701 cttgcccttg agcgctttgg cgaggagctc ttcattattg ccactgttat ggtcggccgt
     5761 atcgatgaag ttgataccga gatcgatagc ccgatgaagc acatcgatat ctcgcctctc
     5821 atcggaatcg ccgagctttc cttggaacgt tggacagcca tagccgatgg cggatacttt
     5881 caatcgttgc tggccgagat accgatactg catgcgacga tgctcctttc cctggagcaa
     5941 ctattgtcct tgccggtgcc gcaatgcaag ttgcaggcaa gaatatttct gctaataatc
     6001 cgagactctg cggacgttta cgacttccat agaaggcgat tgcgtccacc gaaaaccaca
     6061 aaggagatct catgatttat aggcacgttc tctggacctc gatattggca atgtggtttc
     6121 tcctctttac accggcaaag tcagccatcg cacaggagtc attttatcaa ggcaagacgg
     6181 tcagattcat tgttggcggt tcggccggcg gcggctatga cacttacacc cgtttgatcg
     6241 cgcgccatct cggcaagcac gttaccggca atcctaactt tgtcgttgaa aacatgaccg
     6301 gagccggaac tttgatttcg gcaaatcacg tctacaaagt tgccaaaccg gacggcctga
     6361 ccatcggcca tttcattggc ggtttgcttc ttcagcaggt cctgggcaag ccgggcatcg
     6421 aattcgacgg gcagaagttc gagtatctcg gtgtcccggc ccaagacaac acagtcatcg
     6481 gcatcagcaa agccagtggc gtgaccagca tcgacaactg gctggcaacc aagacaacgt
     6541 taaaattcgg cggggtcggc ccgggttcgg ccaccgatga catcgccaag gttgtgcgcg
     6601 ccacggtggg tgtgccgatg cagctcgtca gcggctataa aggcaccgcc gatatccgat
     6661 tggcgatcga cagcggcgag ttgcaggggg ttgccaattc ctgggaatca ttcaaatccg
     6721 gttgggctca ggagattcag tcgggctcgg tcataattgt cttacagatg attcccgaac
     6781 ggcgccatcc ggatttaccc aatgttccaa tgattagcga tatcgttaaa agcgaagagg
     6841 cgcgaaaaat catccaagca ggaatttacg actacagcgc gatcgctcgt ccctacgtct
     6901 ttccgccaaa cacgcccaag gatcgcgtgc tcatgcttcg caagggtctc cttgatacct
     6961 ataaagatcc ggaatttatc gcggatgcgc aaaaggcgcg gctcgacatg agtccactta
     7021 gcggcgagga gttggagaaa gtggtcgctc gcacttataa gcttgagaag ccgattatcg
     7081 agaagttaaa agagatatta aagtaatttc aacgtgccgg gttcgaacag gcgcagtgaa
     7141 acagtatcag gagaatcgat atgaaaaatt ttccaatcat agatgccgat ggacatgtga
     7201 ccgaatcatt ggagagcctc agaaagcact tgcgtaagga gcatcaagca cggccgctct
     7261 tcacttcgga agcttgggac cgatccttcg gcggggccct cggaaaacgc aacgaggatc
     7321 cgaaagttca gcttgccgac atggatctcg atggcatcga tattcaaata gtcttcccga
     7381 ctcacctttc gcttaattca gaaaaggaga ctgctctggc catcgatatt gcgcgtgcct
     7441 acaacgattg gctggcccat ttttgctcgg ttgagccgaa acggctcaag ggagtggcga
     7501 tggttgcctt acaggacgtc aacgctgcaa ttaaggaagt acgccgggcg gttgaagatc
     7561 tcggattggt gggcgtgatg atgccgacca acgtccggga tcaggacatc ggccggcgcg
     7621 agtactggcc gttctatgaa gaagtcgagc gccttgacat cggcttggcc ttgcacggcg
     7681 gcatccgtgc cgccgagcgg atgcatggcc ggttcgataa ctttatcgcc gttcatagct
     7741 tgtcgttccc gttcgaatgc atggcggctg taaccgggct gatctttgcc ggcgtccctg
     7801 agaaattccc caagctgcgc atcgctgctc tcgaagcttg ttgcggctgg gtaccgttcc
     7861 ttatggatcg gctggatgaa gaattcgaga aacggggcgc gaaagaagca ccgctcctga
     7921 agagaaaacc cagcgaatac atgagcggcg aacaattttt ctatgccttt gaactggaag
     7981 agtcgacggt tccctatgtt atcgaccgga tcggccctgg caaattgctt tattcgtccg
     8041 actaccctca ctgggatagc tcctggccca agaccgtgcg aatgtttcaa gaacgtgaag
     8101 acatttccga ggccaataag cggcagatcg catgggagaa cccgcagaac ttttacggat
     8161 tcaaagcagc ctcataacct aacgggaatt tcgaaaagtg ggctcaacaa gggacgaatt
     8221 cgattaattt ctgtgcctca cgaaactatt gtcgagattg gaagagaggt cccactgagc
     8281 cgcagttctg gctatcgtga gctcgaatgg attaaccgag ccggttaaag tttacgccgg
     8341 acaagatgcg caacagtcgc tatttcagca aatagccgaa tttctctcgc gccattctca
     8401 cgacttcctg actcggcgcg ttgaccatgg gaaatttatc cttccagtcg tagggtcggg
     8461 tcgcatcgat caccatgcgg gagttggtga agtccctttt cttgcgctgt tcgggagtta
     8521 gtcgagggtc ggcgggagtg ctgcgcatgc ggcgcaatat gtcgaccgaa gtggccggat
     8581 cgtagcgcga cagcatcgcc cacatcagct ggtccagatt ggagacatcg atatcatcgt
     8641 ccaccacgat caccatcttg cagccgtaaa cgctggcgcc gcaacttgcc gccaaggcac
     8701 cgacctgttt ggcatggccc ggatatcgct gttgaatcgc aagggcgatc agcattcgag
     8761 acgcgccgat ttcatgagcc cagacctgcc ttacgtcggg aacgccggcg ctctgcagtt
     8821 gctgtttgag catggccgag cgcatgatcg cgcggtaacg ggccatctcg tccgagccgc
     8881 cgcctatcgg cggcacaccc agtatgatcg gatcgttgcg atgataaacg gcttcgatct
     8941 cgagcaccgg ctgagcgctt tcatccgatg cataataacc ggtccactcg ccgaaaggcc
     9001 cctcattttt gcgattgtcg ttattcaaaa atccttcaaa gacgatctcg gcattggcgg
     9061 gaaacggtaa gcccgtgagc ggcccgcgca cgacctctac cggtttgcct ctcatgccac
     9121 ctacgatgtc gtactcgcag agaccgtagg ggtgttcggt tcccgccatg aagaaaaaca
     9181 acggatcacc gccaagaacc aaggccaacg gcatcttctg accgcgcttg aaatatttct
     9241 cgcgatgcat gtaaccatgc ttgcccggca ccatcagcag gctgacggtt tttgcgtcct
     9301 ggatcatcgc acggtagcaa ccgacgttaa cccacttctc gtccgggtcc atggttactg
     9361 aatagcaacc tgtgccgata taccggccgc cgtcgttcac atgccacacc ggcgtaggaa
     9421 atttcatgat atcgacatcg ccgcccttga ggacgttttc gaaaattgct ccatccttta
     9481 caaagaccgg cggcatatga ttgggagctt tctgaaaatg atcgaaatac gcttggctca
     9541 attcctgttt ggacaatccc tggggaaagc cgaaggtcat ggcgcgacgt ttgccggcaa
     9601 aagcgttgat caagatgcga aatcctcggg ggcagccggg cacctcgtcg aaaaccacgg
     9661 caggtccatc atccggtgga acaaccacat ccgtggccaa accgatgtct ttctgccacg
     9721 atgcgccaag aaccgttttc aactcaccta gcttttcagc ttcgcgcatc cattcgcgta
     9781 gatccgtaaa tacaatatgc ggctgaatct ttgagctgtc gaacatagtc gctccttgca
     9841 aaggtttgaa ttctcgctca atctaacata gttgtcgtac ccaatggaac ggctctggcg
     9901 gtgagaaata ccatcatttc accgtgtatt gtctcactga aggaagcacc aaagtacttc
     9961 atgatctgaa acgctcattc tttcgttcct ccgtcgaatc ggcaaagtga agcagattag
    10021 tgccggtcga gccaatcccc gataatctta ttagtctcct ccggcttgtg ccagaaaaaa
    10081 ccgtggccac aaccctccac cacggcgaat tcggcgttgt gaatgttatc gcgcagatgt
    10141 tccgaggtga tgacatggct gccggtatcg gcgtcggctt tatcttcgcc gccgacgaga
    10201 accagagtcg gcgtggcaat cttttccagt aattcaccgg tctcatgctg gttgcgggcg
    10261 acgacatggc gtaaataggg atcgagctgc ggccggtttt cccagaaact tttaaataac
    10321 atttccaata cttccgggtg cgctttcacg aactccggcg gaaagaaaaa acggctcttg
    10381 aagtgactca acatgtgttc cctatagccc ttctcgatca tgctcttcag gctatcgacc
    10441 gtcagccagc gcggatagtc gggattgccc atcgaacctg aaccggacgc cgcttgaatc
    10501 atgctgcgca ccttgcgcgg ataatcgatg gcaagccact ggcatactcg ccctcccatt
    10561 gagtgaccga tgatatgagc cggctcattg ataccgagcg catcaagcaa gttagctgca
    10621 tcttgagcaa actgtcgggt cgagtagtat cccgccggcg catccgactt ccccactcca
    10681 cgatggtcat agacgatcac ctgataacgg tcggcaaacg ccggcacttg tttaagcttc
    10741 cacggctccc ctggccaacc cgtgcccgag acgaaaagga acggcactcc acggccgaag
    10801 ctttcatagt acaaacgaac ctcgcccgct tgagcgtatg gcatctaaaa cctcctgaac
    10861 gcatttttct gatgaattgg gctaagcaac ttcaaaaagt aaagctctat gagccagctc
    10921 attagacggt gtggaattta ctggctctac tttcgctatt gctgttgatg taaccacaca
    10981 aaatcggcta atctccagcc aaatatcgga ttgatcgcat tatgtcgata agcgactctg
    11041 actgagtaac cactgaggat tccgaaggcc agcgacgccc ttcccgtaat ttgtcttacc
    11101 gcgagtatgt cagactcagg acaaactgga gtagaaatcg gaaaatatga cttaaagagg
    11161 ttccacttca agctttcccc gccgagctat cgatgatcag aggaaatgga atgaagatct
    11221 taagcgtagt tgttgcgttg atgctgagct ccaccgggtt cgcgttcgcc caggagtcgt
    11281 ttttcaaagg caagaccgtg cggatcgtgg tgccgttcgc ggccgggggc gggtacgaca
    11341 tctattcgcg gattatgggc cggcacatgg gtaagcacat tcccggcaac ccggtcttcg
    11401 tggttgaaaa catgaccggc gccggtggcc tcatcggcat gaatcacgtc tacaaagctg
    11461 ccaagccgga cggcctcacc ctcggcacgc cgatcggcac catgttcgtc gaccaactca
    11521 tcggcaaacc gggcatagaa ttcgacggcc gcaagtttga atatatcggc gcgccggcgc
    11581 aggacacgca gctgctgatc gtacacaagc gcacgggcat taaaacagtg gagcaatggc
    11641 tatcgcacgc aacgccggtg aaattcggca gcaccggtcc gggctccgcc aacgaatcga
    11701 ttccgaagat tgtcaaagaa gcgctaaacc tgccgctgca agttgtttcc ggctataaag
    11761 gcagctcggt aatccggctg gcctttaccg gcggtgaggt cgatggtgtc gccaacgcct
    11821 gggagtcgat ggtctcgacc tggccccaag agattgagag cggcgccgca gtcattttgc
    11881 tgcaggcctc cggaaaacgg catccggatt tacctaacgt tccattggtt atggatcatg
    11941 caaaaacgga tatggctaaa aagctggtgc gcgccgtgat caacaatttc ggcgcaaccg
    12001 cgcggcctta tgtggtgacg ccgggcacgc cgaaaacgcg cgtggaagta ctgcgcaagg
    12061 ctttcatgga taccatgaaa gatccggagt ttggggatga gcttaaaaag gcaaaattgg
    12121 atctgaatcc gctcgacggc gcaaccttgg aaaacaacat caaagagctg ttcgcagtgg
    12181 atcaagcgct cattcctaag ttgacagaga tcctgaaata agaaaggatc gagatcgtgt
    12241 ttctacccgt ttgaatcaaa gcagaaggag ccgaatcatg attgtcgatg ccgatgccca
    12301 cgtggtcgag accgaacaca cctgggatta tctcgatgct tcggaaaaga aattccggcc
    12361 tcaactgttt caatcggccg acaatcccaa tgcgcagtat tggttcgtcg acggcaaatc
    12421 gatcggcttt cgccccccga ccttgaccga acaagaattg atcgcgcttt ccaaatcgac
    12481 cggccgagag ctcaaaaccg ccccagaggc gcgtgagctc cgcgatgtcg agctgcgctt
    12541 gaagcacatg gacaagctgg gcatcgatgt ccagatcctc ttcaacacca tgtggattgc
    12601 ccgggttgcc gacaaggctg aagcagaaat tgcgctttgc aaatcctgga atcattggat
    12661 ggcggatgtc tgggagcaag gcaaaaatcg cttgcgctgg tcgtgcgtcg tcccggcgat
    12721 gaccatcaat gaggcgatcc agcagatgcg ctttgccaaa gagaacgggg cggttgcggt
    12781 gagcctgcgc ccgtttgaag acgataggca tctggtcgat ccttacttct atcctatcta
    12841 cgaggaagcc actcgtttgg atttcgcagt cgcggttcac atcgctaatg ccagcccgca
    12901 gcttctcgat cagttcaaac cgcggtacga caaaatgggc ggcttcgcgc aatttcgtat
    12961 acccacggta atcacctgcc tgtctttgat gatgagcgaa gtgccgcagc agtttccgaa
    13021 attgcgctgg gctttcgtcg aggcttcggc tcaatgggtg ccttgggttg tgaaggaagc
    13081 gatgcggcgg accggcacca aaggatttcc cgacctaccg ttcaaggaat ttcggatcta
    13141 cgtcaccacc gaaaccaacg atgacttcga tttcgtgctg cgctatgccg gcgaagacaa
    13201 cctggtcata ggcacggatt acggccatac cgatgcgtcg agcgaagtgg acgccatcga
    13261 tattttccgg gcaaatggtt cggtgacgga gacggtcaag aaaaagattc tcgacgataa
    13321 tccacgcgcg ctatacacgt tgtagatccc ccctacgcgc tggccaccgc gcttatccac
    13381 ctaccgtcga tgcccaagga tctacgcagc ttcatcgctg aactggacgc tagctcaccc
    13441 gaagatgtag cgcgcgtaac cgaaccggtc tctcctcgtt acgaaatgtc agcaatcctc
    13501 acccatctgg aaaggcgcaa acgctttcca ctgctgtttt ttcaaaattc aaaagaaagt
    13561 caggccccgg ttgtgatcaa cgctcaggct agccgccggc tcatgtccat cgcattagaa
    13621 tgtaaaccgg aggacttagc gcgcgccttt agcgagcgcc aatcaaagcc tttggcgccg
    13681 gtggaaatga tcaacgcgcc ggtgcacgaa atctccagga cgggcgatga gatcgacctc
    13741 aagcaggtgc cgctgctgac ccactatgac gtcaatgtcg ccccttacat caccgccggt
    13801 atcgtcgtgg cggtcgaccc ggacaccgga gtgcgcaacg caagttataa ccggctcatg
    13861 atggcgggca accgcgagct gagaatcttt atggctgtcg gcagacatct gtggacattg
    13921 cacaacaagc ttgagggacg caaccaaccg ctgcccatcg ccatcgtcat tggtgttcac
    13981 cctctttttt ctcttggagc acaagcgctc acgcctacgg atgaagacga gtatgccgtc
    14041 attggcggca tgatgaacga gccgcttcgt ctggtacgag cgaaaacagt tcccattctg
    14101 gtgcccgccg atgctgagat gattatagaa ggccaaatca tgccgaacct gcgccggacc
    14161 gaggggccat ttggcgagtt taccggtcat gccgtgccgc aggatcagcg gcaagtcgtt
    14221 gaaatcagcg ctatcaccca ccgcgagaat tacatttttc aggacattca tgccggctat
    14281 accgagcaca aactgatggg cgcagtgccg cgcgaagctg ctctgttgaa agcggttcgg
    14341 ctgtccgttc ctacagtcag gaatgtgtgc atgcccgtgt cgggcaactg ccgtttccac
    14401 gcttacatct cgatcgctaa acggactcca ggccaagcca aaaatgccat ctgcgccgcc
    14461 tttgccgccg atatgctgct taaacatgtg gtggtcgtcg atgacgacat cgacgtgttc
    14521 gacgagcaac gggtgctctg ggcaatctcc aaccgctttc aagcggaccg cgggctggtg
    14581 gtcattgcca atgctcaagg cagcgagctt gatccttcgg ctggcccggg aggcgttaac
    14641 gccaagatgg gtctggacgc aaccaaaccg ctaagcggct tcccgccgga gctccgagtt
    14701 ccggaagaca ttttggaaaa gattaccctg gacgacttcc ttcctaattt tgcaaaataa
    14761 ttactctgta tttctctatc cgttatcacc gacccgatta ttcggctgcg aaaaaatcgt
    14821 tcacgacacc gtctcgtaga ccagtattag ccgtcaccct ccttagcatt tgacagggca
    14881 tttcatcatc ggctaacctt gcacatctta atcccttctt tgaatgatca gtcaagctac
    14941 ctcatctgaa tggccaacat agagcaggac aaactccggc tcctcaaggg cgcggtccag
    15001 gacaacgtcg acctgctgcg cggaatccgg accaacactc ggcgccggca gtcgaaaatt
    15061 acacgatcgt ggatatggag cccgttgttg ctagcggttc tcgcattttt ttggcagcct
    15121 tcgccggtca tttcgaccaa gtccgcggct aaaccacagg atacgaaacc caacctcaaa
    15181 gaggctccag cgatgacgca atccgctgcg gcggaagttc aagcgccgac aacgccgctg
    15241 catccgctcg atcgctcggt tattccgctt ggcatcaaca gaatcgtcat cgatgccggt
    15301 cacggcggca agcagccagg agcgatttcc gaatccggtg tcttcgagaa ggacattacc
    15361 ctggacattg cgctgcgctt gcaccgcctc cttgagaccg cgccattcga agtgttgatg
    15421 acccggcatg acgaccgaac cataacgctg gataaaagag tggcgtttgc caactcgaac
    15481 ggagcggatc tttttatttc aatccatatc aactggaccg aaccgcgcga gatccgtccg
    15541 ctggaaacct acttcgtcgg ccccagtgag gacccggcga cgatgaagtt ggcaagcttg
    15601 gaaaatcggg aatccggcta ctccttatcg gagtatcgac gagtgttaga aaaaatctat
    15661 atcgatgcgc gccgcgatga atcgcgcagt ctggccaaga acatccatgc tgagctgtat
    15721 caatcgctca aagccgtcaa tccggtgctg gagaatcggg gcgtcagaac ggcgccgttt
    15781 ttggttctgg tcggcacgga aatgccggct atcctcgtcg aagtttcgac gctctcgaac
    15841 gaagaagaag tagagttgtt gatagacccc gattaccggg aaaaaattgc gctggcagta
    15901 tccaaaggga tcaacagcta tgcgaacgcc cttactagat cagctgagaa aggtagctaa
    15961 tgtcaatgga agaaaccaat gaaccgctct gtgtcggaat cgacctcggc acctcgcgca
    16021 gctccatttc cgcatccaat ggtgagcgcc acgtgatcga cagctatgtc ggctggccgc
    16081 tcgatatggt cgcgcgcaag ctgatccaaa aaccgcttct tgtcggtcgt gaagcattgg
    16141 acagccgttc catgctggat ctccgccgtc cgatggaacg cggattgatc aaggatggat
    16201 cgaaacgaga cgaagaagcc gtccaggaga tcctccacca tctaatcaca cagataggct
    16261 tgaaacagaa aaataagacc cgttcaaagg ttcgcgccgt cgtcggcgta cccgcggaag
    16321 ctttcagaaa tagcaaacag cgcttgcgca gcgcgatgga gggagtggcc gacagcgtga
    16381 tgttggtgtc cgaacctttc gccgtggcct atggcttgga agcgttgctg cacgcgctta
    16441 tcatcgatat cggcgcgggc accgctgact tttgcgtgat gcaagggcgt tatccgaccg
    16501 aagaagacca aagaacttta cccaatgccg gcgactcaat tgacgaacaa ctgctgaagc
    16561 tggtgcgcga gcaccatccc gaagcgcaaa cctcgattca tatgattcgt gaatggaagg
    16621 aaaaatggag ctttgtcggt gaagcgaaac aacccgttgt cgtttctgtt ccggtcaaag
    16681 ggaaagccac cgaattgaat ataaccgccg agatgcgcgc cgcatgcgaa agcattttag
    16741 cgcccgtctg tgaaaccatg ttggatctgc tcaccagagt cgagcccgaa taccaatcga
    16801 aggttcgcaa taacgttatt ctagccggcg gcacttcgct catcgttgga ctcagtcagg
    16861 cgctagaaaa ggccctggcc gaactcgggg gcgggcacgc cacagtggtt gaggatcctg
    16921 ttttcgcggg atcggacggc ggcttggcca tcgccaggga tgcatcgagc tcggactggg
    16981 agaagttgtc gctctaacta atctcgacct tcaattcact gctcgcgaaa catcactcct
    17041 gaggcaaaaa gtttcttctg gattctgggt ttcaggaaaa tgcattgacg cgacagcgag
    17101 gctaatcgcg aattgccagc gcgccgccgt cgacaggcag gtcgattgcg tagataaaat
    17161 cggattcgtc tgaggcaagg aaaactacgg ctttggcgat atccatcgga actccgcgcc
    17221 ggccagccaa gtttttgacg aagcgatttt ccggaacttc ttcctgaccc acaggcgagc
    17281 cgattcgatt tggcgatacc gaattgacgc gaatgccttc cggcgccaat tggaccgcga
    17341 tcgatcgggt gaggttaagc acacccgcct tggccgtggt gtaggccgtc gcttctttac
    17401 gtccccaatg gcctgaggtc gaggcaatgt tgatgatctt gcccttgcgc ttgtgatcga
    17461 tcatcacttt ggccgccgat cgcgtacagt accaaacgct cctgagacag acgttcatga
    17521 cgcgatccca ctcttcttcg gtaatatcga ataatgtctt gtgatcggtc caagcggcgt
    17581 tgttgaccag gatgtcgatc gcgccaaatt gcttcaccac cgtctgaacc atttgatcta
    17641 ccgaagccgc cgatgcaaca tcgcatacaa ccgagatagc cgagcctggc ctggtcgcat
    17701 tgatctcact cgcgacacgg ccgcctctcc ccgcatcgag atcgacaacg gccaccttgg
    17761 cgccctcccg agcaaaaagc ttggaaacat cttcaccaat gttgcgccca gccccggtaa
    17821 ccaccgcgat ttgattctgc agtctcatcg tgcctccatc atcgtttttc tgctgaatag
    17881 cagaacccag tttaaaggtc caagtgttgt gaaggatgtc gcttaaagag agctaccgga
    17941 gcgatcgggt caaagactga ccttctcgac gtaaagatct ttcaaaaact tgctattttt
    18001 gagttcttcc aggaaacgcg gttcgataac gtcgcttggc tttatcgatc cagtgcgctt
    18061 atcgatgact gcgccgacat caaccagcgc ctgaagcgac tcgggattga gctccggtag
    18121 cggccgaagc tgagaagaga aactatcata catcgcctga accgatcccg cctgggtatc
    18181 cggaaaatac ttcttgatga tcgccaggct ctcctcacgc cgggtcacca ggtgattgat
    18241 cgcagcggca tagcccttca taaagcgccg gatcgtttcc tcgtctctct taatgaacga
    18301 acgggttgtt accagcaaat ccgtcaccac cggcaccgcc ttcacttcaa gcgttttgat
    18361 gcctaacttt ctcgcttcgg cggcgacttc ttcgctcaac ggcgcgcctt ggacgaggtt
    18421 cttggagatt gccaaagctc gcgcaggcgc gagcccggcg gcgaccattt caacgtctct
    18481 taccgggtca agcccggcgc gtctcagaat gactcgtgca atcaggtcgg atctcgcgcc
    18541 cagcgcagaa acagcgacct tcttaccttt caaatcttta actgtaccga tggatggtgc
    18601 gactcccatc ccgtactggt ccagtctgac atgagaaccg ataacggcaa gatcggcgcc
    18661 gttagccatg ggttggagaa tcgccaatgg actgcctgag ccaatcaata gatcaactcc
    18721 caggagcgcc tgaatcccgg cggacatgtt gtccctgatc ggaatctctt cgatgtcgat
    18781 atcatatttc ttgaacaggc cggcatccac gccgacgtag gcgacggcgg tacctagttc
    18841 ccatccggca taggcgaggc gtaacttctt ggcgccatcc gcggcgaaag caaggcctcc
    18901 cgcaataatt acagacgaaa acaccaacag gaggagcgcc gcgcgtatat ggaaagattt
    18961 tcgattgctc atagctcaga ctatagctca cggacctggc gcttccaagt gagctcctaa
    19021 ccacacgcgt gttccgctcc aatcaataat gcttcggatc gatgcccgta tcttagataa
    19081 cgtcaacaca tcgcgtgccg gaggtgcaat gaaattctcc gtacgatggt tcgagtgaat
    19141 gatttagatg agtccctgag attccactgt taggactcga gagagctaga taagctgacg
    19201 tcggcaagat tgcaaccagg tcacatttaa tttaggattg ttcaggtctt accccacccc
    19261 atccccgtta catccaggag gagcgataaa atggctacga tgattaccaa cgagtgtatc
    19321 aactgcggcg cgtgcgaacc tgagtgcccc aacaatgcca tatcgcaggg tgagaccatc
    19381 ttcgtcatcg atcctcagct ttgcactgaa tgcgtcggct tccatgattt tgaggcttgc
    19441 gccgccgtgt gccccgtgga ttgctgcgta accgacccga acaacatcga aacggaagac
    19501 gccctgattg cacgcgctcg cgcgctgcat cctgagacag tgatcggcga caattttgaa
    19561 tcgcgcttcc gtaaaggaga aggaaaatca ggcgctccaa atggaccgaa atcggcgcca
    19621 gcggcagctt cggcagctgc ggcgatcgcg gcagcgccgc ttacgactgc aagcgcaccg
    19681 cagacatccg gttacgccga gccaacggac aatttcatcg ctttacctcc aatagatgcc
    19741 tgggagattc cgcttcggtg cttcaaatgc tcgcaaaccc atccggagcc cgtgcgcaat
    19801 ttcatgattg gcaacgtcat cttctgccct cattgccaca aatcaatggt cgtgcgggac
    19861 aatctcaatt atcacattcg aacactgcta acggattctt acgagaagtg gcaaaaggaa
    19921 caggaagcct acatcaatcg cagagaaacc gagctggccc agtttatgga aaagcgcgcc
    19981 aaagacgcgc aaggcttcga tgcacatcag cagcgacagt tagaacacat tcgcgggcag
    20041 ttagccgcac tgggcgaaaa ttacgacgcg cccggaaaac cgatcaaaaa aggcgcgcgc
    20101 tttgggtggg gatgattctc gtctgcttta actctgatgt tcaattcgtg gctgcacatt
    20161 atcgctttag ttgcttacct cggcgccatt ctcggtctcg ggctcatgct gttgccatct
    20221 ttgtcttcgg ttgaaaaaca cgaggacaaa gtccatttcc ttgctcgcgg cctaaagttc
    20281 tacaatcctt tacaagtagg agcattgggt ctcgtgctat ttagcggcgc attcgagtta
    20341 acccatctca aagcgatgta tcgtgaaatg tttttgaagg agctcggcta caatttggca
    20401 attaaactca cctttgcttt cttgttggtc ttctttagcg tctaccaagc catggggatc
    20461 ggccaccgct tcgttaagcg tcgggagctc ggcgaaagca tcacgccggc tgagctcgat
    20521 tcagtcgtgc ggcgcctaac cggggcaaat tggctcatcc tatttcttgc catcattacc
    20581 atttggttgg ggctgcggct ccaatcctga ggatcgcggc acgttccgga tctattggcg
    20641 gttacggagc tctggcggag gaggtacgcg taaccgcgaa ggcaattcac cgagccggcg
    20701 tttttaaaaa tgggcaaccg tcaagagatg tttgtcgata ttccgaaaga gcttggcggc
    20761 ttaatcgcgg cgatcgtgga ctggtgcact acttttcaag tggactatct tgagttgaga
    20821 cagctgttga gaggcggcta agaaaaaggg cggggtatct ctaccccgcc ctttttgaca
    20881 tgcacaagct gcttgttact gccgtttgct caaagtctcg gactgcggcg caggtgaatt
    20941 cgcctgaggc gtcgcggcaa ctgcttccga agtgatagcc tcgcggtaaa ccaagatttc
    21001 aactcgacgg ttcttggccc ggttttgcgc attgttgttt tcggctagcg gagcgctttc
    21061 tccataagac accgacataa cttttaacgg atccattttc ttctgagtga tcagatagcg
    21121 actaaccgca tcggcccttc ttttcgcgag ctcgtaatta acgtcatcgc ttccgacatt
    21181 atcggtgtgg ccggccacaa caaaaaccgt attgcccgcg cccgacggat cgcccttaag
    21241 atcgctgagg aacccatcaa tctctttctg agcttgattc ggcaggtttg atgaaccaaa
    21301 cgcgaagtta gcgccctctt tcatatcgat cacaaagcgc cgctcgagtc ttaggttcga
    21361 cagagcgttc aatgccttat ttgctttagc atcgacctgc cctaatctcc cttcgaattg
    21421 gccgagtttg ccttcgattc ccgtgatccg agtgtcaatc gcgccgattt gcttttcgct
    21481 ctgagtcaac cgcccttccc cctgcgtcaa ccggttctcg ctctgcgata ctcggttcga
    21541 taccgggtcc atttgctcct taacccagtc gcgagtcgcg atgcaccctt gggatagaag
    21601 cagagaccct aatgctcccg cactcagaat cgtattaacc ctcataacca atcctccttg
    21661 tttaccaacc gtttgttatt ttctccacat acgtgtttta aaatcactat aggtagctag
    21721 ggcatccaag cgaatcctat gccaggatat ggagcgaatc atatcaagaa cttgcgattt
    21781 taaaaaaatc ttttgcagga aattcctaca tcctcttcgt aggtttaagc cgctgcaacc
    21841 aagttatcga aaccaggatc tactacatgg cgctgtcgca tgacggatcg gctgcttcta
    21901 aggcgggtta agctcaggat gggtgatcgc taaggcagcc gttcatcgaa gcgagcaagt
    21961 ccttccgcga aaacggcttg ttcaatacca gcgcaccatg atgtttccat tcgtctatga
    22021 gagcttcctg atattggctg attgcgccgc tgatatagat cgcctttact ttggggttta
    22081 tcgttcgaat acagcggatc atttctaagc catccaattc aggcatctgt atatcggtga
    22141 gaacgatttc cggctgctga tcgaggtatc tcaaatatcc atgcaagccg tcgtccgcgg
    22201 tttgcacctc atagcccgct gctatcaagg tttcggccat gatttggccg aagttttcat
    22261 cgtcgtcgac aaccaaaact ttgtaaggag cagtcataaa attctcccaa caatggttag
    22321 ctgcgtttgt taatgatccc tgatcctctt gtaacactgc ctgctaaacg caaatcacat
    22381 accacccggt cataaggaca ttggcgcgct ttattttgtg cct
//
LOCUS       DASUVS010000306        11154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1435784, whole genome shotgun sequence.
ACCESSION   DASUVS010000306 DASUVS010000000
VERSION     DASUVS010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11154
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435784"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VF182_16555"
     CDS             complement(<1..619)
                     /locus_tag="VF182_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEX6438751.1"
                     /translation="MSEIFGFDAYAPKEIAERVESIGVTKARLPLLSQATLGVLAGGF
                     IGFGALYFTFITADVSLGFASSRILGGIAFSLGLILVVVAGAELFTGNNFMVMAWASR
                     RISSWELMRNWLIIYCANFIGACGLAVLVFLSNHPEAGAGSVGANAVKIASAKAALPF
                     AEAFFKGIMCNILVCLAVWLSVAGRSVIDKIFAVVFPISAFVAAGF"
     gene            1079..1405
                     /locus_tag="VF182_16560"
     CDS             1079..1405
                     /locus_tag="VF182_16560"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX6438752.1"
                     /translation="MADEKDRFGDTMRLVERAKEDIYFAEQDRQVLEKLRSQLRKVET
                     SDAPRHCPKCPGKLEAYTFEGYMLDRCQECGGVWLDRGELEAIVRKISRGPLGGWLDS
                     LTEKIK"
     gene            1791..2591
                     /locus_tag="VF182_16565"
     CDS             1791..2591
                     /locus_tag="VF182_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438753.1"
                     /translation="MGKRKWQLKALARPLRAKTTMKNEHTKLTVLGLAVLAFAMFPRT
                     LHSYEEIVVKDGATIRGVVKVDGTLPKLPALQITKYKEICKDVPNESLSVGPGQGVRF
                     AVVILEGITRGAAVEKETVYELDNIKCRFSPHVQAASVGQFVLLKNSDPILHTVHAAF
                     TNNQPQFNLGLYPGRTSRKPLVVAGLVTIRCEVHPWMTAYIMVTEHPYHAVSDVYGEY
                     ELSDVPAGVYQLKVWHESLGTQEKRIEVKPAAMKKVDFTFTASPGVKK"
     gene            2588..3211
                     /locus_tag="VF182_16570"
     CDS             2588..3211
                     /locus_tag="VF182_16570"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX6438754.1"
                     /translation="MNKLMLWMFIGVLLGVPASSLAADGGGVGIVKGTITIGGKPATD
                     AVVSIEGLSQDQIKAKMAHTKPQKKIIDQRNLKFIPSVVAIMVGETIDFPNNDKSWHN
                     VYSKGGANDFDLGLYPPGKSRNKKFDKPGVSRILCNAHPNMEAFIVVKDHPFFSTTDS
                     RGNYEIKDAPLGKLRVEIWSPNLDVRSDSVEMARNGEVFALNVELKK"
     gene            3746..4705
                     /locus_tag="VF182_16575"
     CDS             3746..4705
                     /locus_tag="VF182_16575"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6438755.1"
                     /translation="MSRTEGEDEITPEQLRQRQIRNYWAADLSRRVFLMRRQRKAILD
                     NCRRIVVVGASSDPNSRSYLTIEKLLGLGLEIIPIFTDRESFLGLRCFRTLRDVPGKI
                     DIVQTYPNDGIDFAELARAVVEKGVTTCWIEQGLAGSREVEDILAKGHVQLVEYENLE
                     SEYLKHVPLPAATRRPGVPKDEKARKVRERMSKNPVTVKPDDGLKDAIWKMDHGHFRH
                     LPVVDEKEKLIGMLSDRDIRLIRPSLAFSTKEEADVQLWTIAVQQAAVFDPVRVKPET
                     SLKEAADLMLRWHVGGLPVVDDHEHVVGMITYTDLLREFVRRE"
     gene            4946..5662
                     /locus_tag="VF182_16580"
     CDS             4946..5662
                     /locus_tag="VF182_16580"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEX6438756.1"
                     /translation="MFTEKIELKDLDKMDAEYGDLLGRVLTIQADCEIGGPHLYVETM
                     LPTAPSKLDQLIVARTAAEEIDHFRKVARVAGDMGVDVSFVLSQPNEKRFVDSFRGLI
                     TTWENHAVFGFLIDRVGRYQLEEFYDCSYLPLQRILPDIVTEELGHIEYGYNKTLELM
                     SKGDEQKERVQKAIDYWYVRALDMFGHSGSKRAERYLYWGLKRRSNEQAREDYIREVN
                     PILLSLGLKIPDPLEGRRYI"
     gene            5675..5866
                     /locus_tag="VF182_16585"
     CDS             5675..5866
                     /locus_tag="VF182_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438757.1"
                     /translation="MPQAAPDTEKLDEHLLEVVRALHGAVKQGVSHLNDATVMDQAIA
                     DSKEILDVIMAGEVKEWLN"
     gene            5900..6382
                     /locus_tag="VF182_16590"
     CDS             5900..6382
                     /locus_tag="VF182_16590"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX6438758.1"
                     /translation="MSEGKTHFYETEVEWIGAKNLKLSGAKQPAIAAGAPPEFQGREE
                     NWSPEHLLVASLNSCYVLTLIAIAEFSKVGLVSCSSTATGKLEKLEGSGYQITEIIVK
                     PKVVLASAGDLARMPRILGKAKDNCFISNSIKSTIKIEPEVFHHQTQTSPCPLGESPD
                     "
     gene            6513..7442
                     /locus_tag="VF182_16595"
     CDS             6513..7442
                     /locus_tag="VF182_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX6438759.1"
                     /translation="MERSGLTRFAWLSISAALATIALKALAYFVTDSVGLLSDALESL
                     VNLAAALMALAMLVVAARPADEMHAFGYGKAEYFSSGVEGALIVLAAAIIAWTAVPRL
                     IAPQPLEQIGIGLGASIVAAALNFFVARHLLSGSKQYRSITLEADAHHLMTDVWTSAG
                     VVAGVATVALTGWTRLDAVIALCVAVNILFTGVQLLRRSLLGLLDRALGPTEQELIKE
                     ILSRYASQGLYYHALRTRQAGMRSFISFHVLVPGEWTVQRGHDLVEQVERDIRAAVPG
                     ATVFTHLEPINDPAAWQDLTLDRRDDAIRSKCD"
     gene            complement(7514..8458)
                     /locus_tag="VF182_16600"
     CDS             complement(7514..8458)
                     /locus_tag="VF182_16600"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6438760.1"
                     /translation="MDFQLNNIIGKSKAIHDVLELVKKVANTQATVLITGESGTGKEL
                     IALALHALSDRREKLFLPVNCGALPETLLESLVFGHIKGSFTGAFANQEGLFEKARGG
                     TIFLDEIGEIPQHLQVKLLRALEAKEILPLGSTTPRRIDVRVLAATNRNLNNEVEAGR
                     FREDLYYRLNVMEIHIPPLRERPEDIPELIEHFIKRHNPELKRNFQGIDEAAVRVLMA
                     LQWKGNVRELDNVIEHTMILAEGDRIMLKDLPSAIVASSQTNPAFTFNLRDALRQFEK
                     QHIVRALDQANQDRKEAARLLDISLSSLYRKIEELEIS"
     gene            complement(8607..9110)
                     /locus_tag="VF182_16605"
     CDS             complement(8607..9110)
                     /locus_tag="VF182_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438761.1"
                     /translation="MSTSAPFDPLLRFAEIERLVSKIYYRFSHLFLARQELRDFWWEM
                     AREEEQHANILGACRELILNYNDDSLDPMISSDSADKVAAGLQSFLDQGTPTLAIEDA
                     FRIALEIESSEIDVIYGKLLQLGGPQIGKTMESLGVPAAVQRQKIKAALRRFCSDPEL
                     LQAAERF"
     gene            complement(9416..9703)
                     /locus_tag="VF182_16610"
     CDS             complement(9416..9703)
                     /locus_tag="VF182_16610"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6438762.1"
                     /translation="MLECHQQSLDKFLREKFPDEMNLTEVRQVVEFGGAVDNIIEKAE
                     AEEVDRIVMSTHGRTGIDHMLIGSVSEKVIARASCPVLIMPAHRRKIARAA"
     gene            complement(10206..10712)
                     /locus_tag="VF182_16615"
     CDS             complement(10206..10712)
                     /locus_tag="VF182_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438763.1"
                     /translation="MEEFADVNALSEFMATREGISPIERLLNEFEAHEAKEDHALEFY
                     KETLAHMPSPLTRFLVQLIVSDEEKHRAVVHAMVSTLKGSLTWTRPADSLENTGDDTV
                     STKELRNVTDELIRLEKDGIAEYKTLVKASSGYYRGVFKILLDSMIRDSEKHVELLEF
                     LKQTLKEA"
BASE COUNT         2709 a         2868 c         3049 g         2528 t
ORIGIN      
        1 cgaaacctgc ggcgacaaac gcggaaattg gaaacacgac ggcaaaaatc ttatcgataa
       61 cgctccggcc ggcgaccgac agccagacag caagacacac caggatgtta cacattattc
      121 ccttgaagaa cgcctcggca aaaggaagcg ccgccttcgc agaagctatt ttgactgcgt
      181 ttgcccctac cgatcctgcg cccgcttccg ggtgattaga caaaaagacg agaaccgcaa
      241 ggccgcacgc gccgatgaaa ttcgcgcagt agatgatcag ccagttgcgc atcaactccc
      301 atgagctaat gcgccgactc gcccatgcca tgaccataaa gttattgccg gtgaaaagtt
      361 cggcgccggc gaccacaacc agaatcaaac cgagagaaaa ggctatgccg cctagtatcc
      421 ggctggacgc aaatccaagg ctgacgtcag cggtaatgaa agtaaagtaa agcgccccga
      481 acccgataaa cccgccggcg agcacgccaa gcgttgcctg tgaaagcagc ggcaggcgcg
      541 ccttagtgac accaatgctt tcgacgcgct cggcgatttc cttaggagca taagcatcga
      601 agccgaagat ttcactcatc ggcgttcacg ccgttcggac ccttcggctg tgaaagcggc
      661 gaagagtctc cgggttgcaa cgatacgccg gcaggatggc gctccggtag aaattgcgca
      721 gtcatactca ttccttcggt tagcttaagt actggtgaaa acggtcccat ctcgactctg
      781 gtctattttg gtattttggc atcaaggcac tcgctccgaa ggaaggtaac ccaattagca
      841 aaaaggcaaa agagatccct ggatgagccc tgcgaaaaga actgccgaaa gtccaagcga
      901 aaacgaattc tcaaaggcgc gaaaccagcg accgatcttt agcgattcta gtaattccgg
      961 ctaggtagag acaccgcccg ccggcatttt gcctggtacg aactcgataa aaagtagtgt
     1021 gatcgtaagg gaaaccggca tcgaagatgc gttatttcct ttgtgagagg tgagaaatat
     1081 ggcagacgaa aaggatcgat ttggcgatac catgcgactt gttgagagag ctaaagaaga
     1141 tatttacttc gccgagcaag atcgacaggt gctggaaaaa ctccgcagtc aactccgtaa
     1201 ggtcgagact tctgacgcgc cccggcactg tcccaagtgc cccggcaagc tagaggctta
     1261 cacgttcgag ggttacatgc tcgaccgttg ccaggaatgc ggcggagttt ggttggatcg
     1321 gggtgagcta gaggcaatcg tcaggaagat tagccgcggc ccgctcggtg gatggctgga
     1381 tagcttgacc gagaaaatca aatagtgagg agcttggaat gagatcgggt ggcgaaaaaa
     1441 tacaacacct ggtgacgagc ttgatgcgct tcgaaaggcg cgcggcaact ccttttaggg
     1501 tttcggtagt aagttgattg cgcaacgtag tggggactgg cgcctgaagt gtatcgagac
     1561 catcaatggc gtgatccctg cgctacgaaa cattttcatg aatccatgaa tccacgacag
     1621 agcgatagtt catctcgccg cccgtatgcg caggccagac gtcttcacac ctgcaaaaag
     1681 ccgtaaaaat ttgctcaact ttaagaaaat catccgctat taaactgcgg catgactgcc
     1741 agctcagctc gcgcagaccg gatgtttctc ccggaaaaca tagcaacgga gtgggcaaac
     1801 gtaaatggca gctcaaagca ctggcacggc cgttgcgggc aaaaactacc atgaagaacg
     1861 aacacacgaa gttgactgtc ctgggcttag ctgttttggc attcgcgatg ttcccacgga
     1921 cgcttcattc ctacgaggaa atcgttgtca aagacggagc gaccattcgc ggcgtcgtga
     1981 aagttgatgg tacgttgcca aaactgccag cgctgcagat tacaaaatac aaggagatct
     2041 gtaaggacgt cccaaatgaa agcctgagtg tcggcccggg acaaggagtt cgctttgcgg
     2101 ttgtgatcct agaaggcatt accagaggtg cagccgttga gaaggaaacc gtctatgagt
     2161 tggataacat caaatgtcgc ttcagtcccc atgttcaagc cgcgagcgtc gggcagttcg
     2221 tattattgaa gaattccgat cccattctcc acacggtgca tgctgctttt acgaacaacc
     2281 agcctcaatt caatcttggg ctttatcccg gacgcacaag ccgaaaaccg ttggttgtag
     2341 cgggtctcgt cacgatccgc tgcgaagttc acccgtggat gaccgcttac atcatggtca
     2401 ccgagcatcc ttatcacgcc gtttcggacg tctatggaga gtacgagctc agtgatgttc
     2461 cagcgggtgt atatcaattg aaagtgtggc acgagagcct gggcacgcag gagaagcgga
     2521 tcgaggtaaa accggcggcc atgaaaaaag tcgacttcac tttcacagca tcaccggggg
     2581 taaaaaaatg aacaagttaa tgttgtggat gtttatcggt gtgctgctcg gtgttccagc
     2641 gtcaagccta gcagctgacg gcggtggcgt gggaattgtc aaaggaacga ttactatcgg
     2701 tggcaaacca gcaacggatg ccgtggtctc gatagaaggc ctttcccagg accagataaa
     2761 agccaagatg gcgcacacca agccacagaa aaaaatcatc gatcaacgca acctcaagtt
     2821 tattccgtct gttgtagcga ttatggtcgg agagaccatt gatttcccaa acaacgacaa
     2881 gtcttggcac aatgtgtatt ccaagggagg cgcaaacgat ttcgatctcg ggctctatcc
     2941 gcccggtaag agccgaaaca agaagtttga taagccgggc gtctctcgaa ttctgtgcaa
     3001 tgcgcatccg aacatggaag ctttcatcgt cgtgaaagat cacccgtttt tttcgaccac
     3061 ggacagccgc ggcaactacg agatcaaaga cgcaccttta ggtaagctgc gcgtcgagat
     3121 ttggtctcca aacctggatg tcaggagcga cagcgtcgaa atggcgcgca atggcgaggt
     3181 gtttgcgcta aatgtcgagc ttaagaaata gaagtgatct gagcactagg catgagcgaa
     3241 gaaatttgag tgccttagcg taaacgtgcg tgactgttcg aagtggtgag ggattactca
     3301 tcaattgact gaaacgactg tgtgccagcc gtttgtgttg ccgcaccgtg acggactacc
     3361 tcggcttcat gcgtgacgcg tcgggctcaa acggctaatt cttaataccc atcggatgct
     3421 gcaacagtac ttggtgaagg cttgattcta gtttgcggat tgagcaatca tcgctcgagc
     3481 gccgcgccgg tgaatcgtgt cagcacgttc actctggccc gcgcacgtct ctaggtgttt
     3541 cggcgccgag atcggatccc gccggattac agagcatatt cctagcgctg caaattgaca
     3601 gagtagcttt ttgcgaagtt gccaacgatg ggagctcgga attggccaga accatgcttt
     3661 tgaatggtat ggcgcttgct cccttgcaaa tagaagctgt caccgcgcgt gtggatctca
     3721 gcggggctcg aagggagcag gctatatgtc tcgaaccgag ggtgaggacg aaattacgcc
     3781 tgaacaactc aggcaaagac agatccgtaa ctactgggca gcggacttaa gccgaagagt
     3841 ctttttaatg cggcggcaga gaaaagccat tctggacaat tgtcggcgca tcgtggttgt
     3901 gggcgcgagc agcgatccaa acagtcggag ctacctcacc attgagaagc tgcttggcct
     3961 cggcctggaa atcattccaa tatttaccga tcgcgaaagc tttcttggct tgcgctgttt
     4021 tcgaacgttg cgtgacgttc cgggcaaaat cgatattgtt cagacctatc ccaacgacgg
     4081 gatagatttc gccgagctgg cgcgggcagt ggtggaaaaa ggcgtgacaa cgtgttggat
     4141 cgagcaagga ctcgcgggct cacgagaagt cgaggatatt ctggcgaagg ggcacgttca
     4201 gttggtcgag tatgagaatc ttgaaagcga gtatctaaag catgtcccgt tgccggcggc
     4261 gacacggagg cccggagtgc ccaaggatga gaaggcgagg aaagtaaggg aaaggatgtc
     4321 aaaaaaccct gtgacggtga aaccggatga tggactgaaa gacgcgatct ggaaaatgga
     4381 tcatggccat ttccgtcacc tgccggtggt ggatgaaaag gagaagctga tcggcatgct
     4441 gagcgatcgc gacattcgtc tgatccgccc ctccctcgcg ttctcaacca aggaagaagc
     4501 cgatgtgcaa ctctggacga ttgcggtgca acaagccgcc gtgtttgatc cggtccgcgt
     4561 taagccagaa acgtcactta aagaagcggc cgatctgatg ttacgctggc atgttggcgg
     4621 gctgccggta gttgatgatc atgaacatgt cgtcggcatg atcacgtaca ctgatttgtt
     4681 acgggagttc gtccggcgcg agtaggcaca gaacgcgcgt caggtcttag gcactagtat
     4741 ggtattggtt ccgatgaaag ccaatcacag gggcggttga aaacctttct cattcttcag
     4801 aaacaaaact ggcgcctctc caaaatacga gaacaatatc tgaaaattcg aagcttgtcg
     4861 ggcggcgaga ctggcacttc tattgctcct tgtattggag agaattgatc ttaatcgaca
     4921 aactagggag atgatggggt aaagtatgtt taccgaaaag atagagctca aagatctgga
     4981 caagatggat gcggagtatg gggatctact ggggcgcgtg ttgacgatac aagcagattg
     5041 cgagatcggc ggaccgcacc tttatgttga aacgatgctg ccaacagcgc cgagcaagct
     5101 tgatcaactg atcgtcgcgc gcacggcggc cgaggagatc gatcatttcc gcaaggttgc
     5161 gcgagtggcc ggggatatgg gagtcgatgt ttcatttgtg ctgagccagc ccaacgaaaa
     5221 gcgtttcgtc gactccttcc gtgggctgat cacaacgtgg gaaaaccacg ccgtcttcgg
     5281 ctttttgatc gatcgggtcg ggcgatacca gcttgaagaa ttctatgatt gcagttatct
     5341 gccgctgcag cgaattcttc cggacatcgt caccgaggag ctgggtcaca tcgaatacgg
     5401 ctacaataaa accctcgaac tgatgtccaa aggtgacgag caaaaagagc gcgtccaaaa
     5461 ggctattgat tattggtatg ttcgggcgct cgacatgttc ggtcacagcg gatccaaacg
     5521 cgccgagcgt tatctttatt ggggactaaa aagacgcagc aatgagcagg ctcgcgaaga
     5581 ctacatccgt gaagtcaatc cgatcctgct tagcttgggt ctcaaaattc ccgatccgct
     5641 cgaaggccgc cgctatatct gacaggagat tactatgccg caagcagcac ctgatacgga
     5701 aaagctcgac gagcacctac tcgaagtcgt ccgcgccctg cacggggccg tcaagcaggg
     5761 agtttcccat ctaaatgacg ccacagtgat ggatcaagcg attgccgata gtaaagaaat
     5821 tctcgacgtc atcatggcgg gagaggtaaa ggagtggcta aactgagtcc ggcggaggtt
     5881 cagcgctcag ggacatttta tgagcgaagg aaaaactcat ttctacgaga ccgaagtcga
     5941 gtggatcggc gcaaaaaacc tgaagcttag cggcgccaag cagccggcga tcgccgcagg
     6001 agcgccgccg gagtttcagg gccgggagga aaactggtcg ccggagcatc tgctggttgc
     6061 ctctctgaac agttgttatg tcctgacctt gattgcgatt gcggaattct cgaaagtcgg
     6121 tctcgttagt tgttcctcca cggcaacggg aaaattggag aagctcgaag ggagcggtta
     6181 tcagatcacc gagatcatcg tcaagccaaa ggtggttctt gcgtcggccg gcgatctcgc
     6241 gcgcatgccg cgaatcctgg gaaaagccaa agacaattgc tttatctcca actcgatcaa
     6301 gtcgacaatc aagatcgagc ccgaagtctt tcatcatcag acgcagacat ctccctgccc
     6361 tttgggagaa tctccagatt gacgcatctg ccggtcagtc gctaaccggt ctcttaatat
     6421 cttcatcgac cacgctcttt tttggttcac aaccgttcga aaacaaaaga cgaaagcata
     6481 aaatctaact caggggtctc ttgttgatcc aaatggaacg atccggtctg actcgcttcg
     6541 catggctctc gatcagcgcg gcgctcgcga ccattgcttt gaaggctttg gcctatttcg
     6601 tcaccgattc cgtggggctg ttgtcggatg cactggagtc attggtgaat ttggctgcgg
     6661 cgctcatggc tcttgcaatg ctagtcgttg cggctcggcc ggcagacgag atgcacgcct
     6721 tcggctacgg taaagccgaa tatttttcca gtggcgttga aggggcgctg attgtccttg
     6781 cggcggccat cattgcctgg acggcggtgc cacggttgat tgcgccacag ccgttggaac
     6841 aaattgggat cggtcttggc gcctcgatcg tagcagcggc gctcaatttt ttcgttgccc
     6901 ggcatttgct cagcggcagc aaacagtatc gctccatcac acttgaagcc gacgcccatc
     6961 acctgatgac cgatgtctgg acttcggccg gtgtggtcgc cggagtcgcc actgtcgccc
     7021 tgaccggctg gacacgtctt gatgcggtga ttgcgctgtg tgttgccgtc aatattttat
     7081 tcacgggagt gcaactgcta cgccgctcgt tgcttggact tttagacagg gcgttgggac
     7141 ctaccgagca agaacttata aaagaaatcc tcagccgata tgccagccag ggtctttatt
     7201 atcatgcgct gcgcacgcgg caggcgggaa tgcggtcgtt catatccttc catgtgctcg
     7261 tgccgggaga atggacggtt cagcgcgggc atgatctggt cgaacaagta gagcgcgata
     7321 tacgcgcggc ggttcccggc gcaacggtct tcactcacct cgagccgatc aatgatccgg
     7381 cagcttggca ggacttaacg cttgatcgga gagacgacgc aatacgcagc aagtgcgact
     7441 aaaaggcaca aatgatccgt tcggctatcg atttgatgac gatcaagaca aggatcgagt
     7501 cgataaaagc ttgtcagctg atctccagct cttcgatttt gcggtagagc gacgagagac
     7561 tgatatccag aagcctggct gcctctttgc ggtcttgatt ggcttggtcc agggcgcgca
     7621 caatgtgctg tttttcgaac tgtcgcaatg catcccggag attgaatgtg aaggcgggat
     7681 tggtttgact actggcgacg atcgcgctcg gcagatcctt caacatgatt cggtcgcctt
     7741 cggcgagaat catcgtatgc tcgatgacgt tgtcgagctc gcgtacgttg cctttccatt
     7801 gcagcgccat gaggacgcgg accgcggcct cgtcgatacc ctgaaagttc cgtttgagct
     7861 cggggttgtg acgcttgatg aaatgttcga tcaactcggg aatatcttcc ggccgctccc
     7921 gtaaaggcgg aatatggatt tccatgacat tgagacgata gtagagatct tcacgaaagc
     7981 gacccgcttc cacttcgtta tttaagtttc gattggtcgc cgccaaaacg cgaacgtcga
     8041 tccggcgcgg tgtcgtgctc cccaggggaa gaatttcctt ggcttccaaa gctctgagca
     8101 atttgacttg gaggtgctgc gggatctcgc cgatttcgtc gagaaaaatg gttccacccc
     8161 tggccttttc gaacaaacct tcctggttgg cgaaagcgcc ggtgaaagaa ccttttatat
     8221 ggccaaaaac aagactttcc agcagcgtct ccggtaatgc gccgcagttg accggcagaa
     8281 aaagtttctc gcggcgatcg ctaagcgcgt ggagcgccag agcgatcagc tctttgccgg
     8341 tgccactttc gccggtgatg agaaccgtgg cctgagtgtt ggcaactttc ttgaccagct
     8401 ctaggacgtc atggatcgcc ttgcttttgc cgatgatgtt gttcaattga aagtccattg
     8461 acctttaagc cgaggtgcag tcatcggcag gtcacgccat ttgcgtcctt tgcgcgcgaa
     8521 tcctctcctt acaagatgcc ggcagcacgc catcggcaag ataaaagcgc gcgatgaatg
     8581 tgtcaaggac ggaggaggga ttggacttag aatctctcgg cggcctgaag cagctccgga
     8641 tcggaacaga aacggcgcag agcggccttg atcttttgtc tttgcaccgc cgcgggaaca
     8701 cccaagcttt ccatggtttt gccgatctgc gggcctccca gttgcaacaa tttgccgtag
     8761 atcacgtcga tctcggagct ttctatttct agcgcaattc tgaacgcatc ttcgatggcg
     8821 agcgtcggtg taccttggtc aagaaaggat tgcagtccag cagctacttt atcggcactg
     8881 tcactgctga tcatcggatc gagggagtcg tcgttatagt taaggataag ttcccggcag
     8941 gcgcccagaa tattcgcgtg ctgctcttcc tctcgcgcca tttcccacca gaaatcacgc
     9001 agctcctgtc gggcgagaaa gagatgcgaa aaccggtagt aaattttcga aaccagtcgc
     9061 tcgatctcgg caaagcgcag cagaggatcg aacggcgctg acgtactcat ggcttttaga
     9121 ttcctatcac aaaattaagc ggctcatcat gcttctcgga atcgcgtcgt gcagctagcc
     9181 ggttgcaaac ggcatccgaa tcgcagaagc gccactccaa cagaatttca gtatatcacc
     9241 cgccgaaaac gccgccaccc cactaaggcg cccgatccca atcccgcggc accggatgtt
     9301 cgccgaatgg tgaactataa acccgttgtg cttcggcgtt cacgcgtcga gactggcttt
     9361 ccggcggtcg atctaagcat ttatttaccg catcatgccg gtaatttacg atcaattagg
     9421 cagcccttgc gatcttgcgc ctgtgcgccg gcatgatcag gaccgggcat gaagcgcggg
     9481 cgataacctt ttcggaaacg ctgccaatca gcatgtgatc gatgccggtt cggccgtggg
     9541 ttgacataac gatcctgtcc acctcctcag cttccgcctt ttcaatgatg ttatccacgg
     9601 cgccgccgaa ctcaaccacc tgccgaactt cggttagatt catctcatcc ggaaacttct
     9661 cgcgcaggaa cttgtcaagc gactgttgat ggcactccag catctcacga accggcccaa
     9721 gaatgctcgg gcttgcccca ttgatcggct aagtcaatga cgtgatatac gatcacctct
     9781 gcgccgcgca atcgtcccat ttccagcgcg tgtcgcagcc ctatgcagga aagctccgag
     9841 agatccgtcg gcgcgagaat ttttttattg tttccattta accctccggt gcctctactt
     9901 gattaaaggt gggtagcgca agaaccatac cgctaccggt cagggaaact acttcagaat
     9961 tgcacgggag agtcgtttag aatcgcaaat gttagaatcg gcgcggattc tttctcgtgc
    10021 aggcgaagtc agcattgatg cacctgccac cgcggactca ccgtttccgg gtgtttgaca
    10081 caaccggttc caatcccgcg atcatcgcgg cgattggaag gttattcctt aaagactttc
    10141 gcagtcgatc ttgcggtgtg cgaccggccg ttcgatggca atggatcgaa acggcttccg
    10201 cgtattcatg cctctttcag ggtctgcttt aggaactcga gcagctccac atgcttttcc
    10261 gaatcacgga tcattgaatc caaaaggatc ttaaaaactc cgcgataata gccgctgctc
    10321 gccttaacta gagtcttgta ttccgctatc ccgtcctttt cgaggcgaat cagctcgtcg
    10381 gtgacgttcc gaagctcctt ggttgagacc gtgtcatcac cagtgttttc aaggctgtct
    10441 gcaggcctag tccaggtgag actccccttt agcgtcgata ccatggcgtg caccaccgcc
    10501 cgatgtttct cttcgtctga aacgataagt tgtacgagaa atcgcgttag cgggctcggc
    10561 atatgcgcca aagtctcttt gtagaattcg agagcatggt cttccttagc ttcatgggct
    10621 tcaaattcat ttagtagtct ttcgatcggg gatatgccct cgcgggtggc cataaactca
    10681 gacaaggcgt ttacatctgc gaactcttcc atcatcgtgc tccccctttc acttcttcct
    10741 gattaacacc tgcgcaaagc acatgccaca aaacacgcaa aaatgcttgg gtctgacgga
    10801 cgatgaatga gaatattctt agacatgaga atcttagtca ttcgcttcgc gaatctgcaa
    10861 aaactttcgt taggacgatc ggtggaataa gcagaaaaaa aggcggtggc gccgatcatc
    10921 atctgaactc atgccgcacc tttgtgatcc gccttttttt tcgaaatgcc cgcaaacaca
    10981 tgtacccagg tgaacgccta atagcccttc tccaggcacc agttggcgat ttcaatgcgg
    11041 cgtgcgaacc agaccttggg aaagcccttg gcatagcgaa tgaactcgtc gatgattttg
    11101 gttcgaaacg gcacgccgga aacaaacggg tgcaggttga aagcaaagaa cttg
//
LOCUS       DASUVS010000307        14609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1219434, whole genome shotgun sequence.
ACCESSION   DASUVS010000307 DASUVS010000000
VERSION     DASUVS010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14609
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219434"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..860
                     /locus_tag="VF182_16620"
     CDS             141..860
                     /locus_tag="VF182_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438764.1"
                     /translation="MARDKFLWCRKCDAVHHVTSWDRAPQYNYNNCIDGELDEIPADD
                     WRAFMKEHEGHRLEPLEASGEKYFCQGPMSDPMGVAYIEVTNGRDHLLLRQSRKSIAE
                     PLRFELTRDRLIDQGVTVGIQDSEIKKEMRHHFKWSGGPFEDQKIDLFIQLLRNVVTD
                     LEPRAIEVAGFCSEDHNVSFGSLQAEVIESLMAECALCFQPQELDDIRRFIDSHREPS
                     DVLALVMRRQLKVEQSAAAIK"
     gene            complement(992..1531)
                     /gene="pyrR"
                     /locus_tag="VF182_16625"
     CDS             complement(992..1531)
                     /gene="pyrR"
                     /locus_tag="VF182_16625"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX6438765.1"
                     /translation="MKKSKNVRLLTASEIKRTLERMAREILRQNRASDSLAFIGIRTR
                     GLFLAQRVRGIIQKSQHKDIPLGEMDISLYRDDLNTLLPEGKVDHTSIPFDINNKTIV
                     LFDDVLHTGRTVRAAVDHLIDLGRPRAIHLAVLIDRGGREFPIAANFVARKIILPQDG
                     EVRLKLKEVDGRDEVVVKK"
     gene            complement(1518..2813)
                     /locus_tag="VF182_16630"
     CDS             complement(1518..2813)
                     /locus_tag="VF182_16630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HEX6438766.1"
                     /translation="MKSQTPNAIRSQTDITINKRRLLRDLNAVSRIGLGDHGSVTRLV
                     FSIKELRSRQFLIHQMRQIGLQINIDRVGNIFGRLDGPHPRAPAVLAGSHLDTVVHGG
                     KFDGTMGVIAALEAVRTLKEQNVALRSPVEVVCFVGEESSRFGFSTLGSSLVAGEVRA
                     DDLANASDPQGAKLEDILAGLGIHRNNLRSLRRDPSSLKAYLELHIEQGPILEARKKP
                     IGVVTSIAAPTRFKVIITGQADHSGTTPMEMRKDALVAAAELTVAIEGICRQHSDIDK
                     GRVVGTVGALRIEPGVINAVPGKAELAVDVRSITASAKNRVVRLIEAKIREIARRRSI
                     RIKILPIRKEDPVPLDKRIVHLLQDCCEIREVAYEIMPSGAGHDAMQMAKITPAGMLF
                     IPSRRGISHNPLEWSAPQDICLGAQLLLDAIVRASNEKI"
     gene            2934..3602
                     /gene="pyrE"
                     /locus_tag="VF182_16635"
     CDS             2934..3602
                     /gene="pyrE"
                     /locus_tag="VF182_16635"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX6438767.1"
                     /translation="MSEVSKKVAEMLLSTESIQVYKETPFVFVSGRISPVYIDCRKLL
                     SFPMEREFIVAALAKMVEAEIGTDKIDVVAGGETAGIPYASFVAHMIRKPMIYIRKQP
                     KGYGGTKQIEGILNPGKKVLLVEDLVTDGLSKLRFNIGIRGAGAKMTHCLCVFDYASD
                     RLNQHEGKENLAKNDISLHVLANWDDVLDTGLAKSYFSETASKHITDFLQDPENWGRK
                     MGFV"
     gene            3737..3813
                     /locus_tag="VF182_16640"
     tRNA            3737..3813
                     /locus_tag="VF182_16640"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3771..3773,aa:Val,seq:cac)
     gene            5831..7042
                     /locus_tag="VF182_16645"
     CDS             5831..7042
                     /locus_tag="VF182_16645"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX6438768.1"
                     /translation="MNRSRKVLLSDLCRVAGGTPAGDWFRCYWLAVGTAGELRDVPRA
                     VRVLGEELVLFRDDRGRIGLLGLHCPHRGSSLEYGDIENGGIRCPYHGWLFDVSGQCL
                     EQPAEPEGSIFCRKVKHLSYPVAELGGLIFAYMGPGQESPPPLPQYKALVDPNGQRSL
                     EATREYDYNWFNFIENGADPAHFSILHRTDPNDGTWRSWFFNFKDVPPFDAVETSYGM
                     KVISRKPGPAADTEYVDEKSFALPSILQIGDTEFTHFRKPKEALAAGSHNAHFMFVTP
                     SDDDHFTLFTVNHYTGPDPEFFHKLEPSRRIVPKMEKKEYDQRRYSPFRGSVRSEDIM
                     SQSTQGTVAERKEQLATSDKGVILLRKIILGAIETMQKGEVPKGVPLREDSAKVIDID
                     SFTGIRPKGVF"
     gene            7044..8036
                     /locus_tag="VF182_16650"
     CDS             7044..8036
                     /locus_tag="VF182_16650"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438769.1"
                     /translation="MKLSVIMSARFLTSVFLGLFLVASAVEAAPLRIAYSAISGAMSS
                     LWVAQEAGYFKREGLDVELLYVGGGSLLIQSMISGDVPFAYGPSVPVINASLRGADLV
                     LIGNTSNSLVFSIISRPEIKQSANLKGKKVGVTRLGGSTDWALDAALKQWGLERREIT
                     VIQTGGMPEGLAGLMAGVFDAVVLSPPSNFRAVKAGMHELVDVGQLRIIFPNTPLSTT
                     QSLIKGNRDSALRFMRGFTQGLHRLRNDKEFSMKVLSKYTKVTDNETLAQLHQTYGVR
                     YSGDRIPYVRTEGVDEILKRTPGKEAREAKAGDFVDNTLLRDLEQSGWFKMLER"
     gene            8040..8924
                     /locus_tag="VF182_16655"
     CDS             8040..8924
                     /locus_tag="VF182_16655"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6438770.1"
                     /translation="MRVVVTGGSGRLGQYVVRELFTHNHAVAALDAIKPRECLCPTYT
                     VDLMRIDSFVDHLNNADALVHLARVRFPYTENGYNVAEQKWEFADIAGDAERFNRNVA
                     MTNNVLAAAQACAIKKAVCGSSLAVYGFYYPSTGLQPEHLPVDEDHPLRPQDPYGISK
                     LIVEKLCDALSHKTGAQVASLRFAGIYTEAHRSMLLDRKKNPLARGTGALWSYIDARD
                     AARACRLALETDFGGHQAFNICASSTIMDTPTRELIARYLPRVKVLRSGLDDRASGYS
                     IEKAKKFLSFEPRCSLVN"
     gene            9053..10429
                     /locus_tag="VF182_16660"
     CDS             9053..10429
                     /locus_tag="VF182_16660"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6438771.1"
                     /translation="MMDLREFLKVLEEEHELQKIKVEVDPKHELGAICKIHNERSNSP
                     ALLFENVKGHTIPVVGQLLASDRRVALALGLSQENVFNETVQRASNPIAPKLVSKGAS
                     QDIVLEGAGLDLTKLPLCTNNPRDGGPYITAGHVIIKDPEYGMNLSIYRMMLVSKNEV
                     TLRFTPGHDGYDFMKNAEKRGQRKFEVAVCIGVPPAVYVASQFEPRLGVYELDIAGGL
                     VGEPVEVVKCRTIDLEVPALAEIVLEGELTIPAKTGDEGPFGEFCGYTTAQVPNERIM
                     TVKAVTHRKNPVYHNIWLGKPPHEHLYVDALTYAVAAYQELKPAYPALKKAYAPPWGV
                     SIVLLLQLEKRLMRPGIVDNILAASLYTRSGKWKHVFVLDEDIVLEDPNEVLWALTTR
                     FQPATDMFIIPRGITSSLEPSATVDGLTSKLMLDLTIKKNFRGEVAEPTASMRESVLK
                     RWKEYGFK"
     gene            10545..11756
                     /locus_tag="VF182_16665"
     CDS             10545..11756
                     /locus_tag="VF182_16665"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX6438772.1"
                     /translation="MKKVTPADIPRVGPGTPAGEWFRKYWLVVGTTQELHDIPQAVKV
                     LGEDLVLFRDEFGKLGLLGLHCSHRGSSLEYGDIEDGGIRCPYHGWLFNVAGQCLEMP
                     AEPKDSKFPQKVKHLSYPVREQGGLIFTYMGENQNDPPPLPKYSPLVDRGSQWQIEPV
                     RHADYNWFNFFENSADPAHVCILHRNAGYGQQSWGNQFFSYTDMPDFEFVEMDYGMKV
                     VMTKPGPEPGTEFVDEMSLALPSIVQVGDTEFVHAKIDSAALVKEGSQCEHWMFVTPN
                     DDDHFMLFTADNYRGPEENFFEKLKALRAKEMPAQEVKPYDKRPFMPYKGNIRKEDLV
                     TQGTQGLLGERSEQLGVSDRGVIKFRKIVVDAIETALNGGTPKGIVDKKEESEVYRLD
                     TQVGVRAKASN"
     gene            11910..12800
                     /locus_tag="VF182_16670"
     CDS             11910..12800
                     /locus_tag="VF182_16670"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX6438773.1"
                     /translation="MIRGVLLPVITPFDEKIRVDEQMMRQLVDFHIKAGVQGLFVLGS
                     TGQGPAMTIEERKQTAAVALDQAKSRVPVIIHVGTADAGSAVELAEHAAAHKADAVAI
                     VPPYYYSDHTEYEIIAHYKAVHKAVPLPIFIYENPKYSGISIPPGLAVRMKEQVPALE
                     GIKVAYGQGALLEYVRLLPDISVFTGNADLFGLVPFGVAGMINPPTSFVPELCVDLFK
                     ALDSQDYPRAVAAQKRVDTAARIVAARLRKFGRVPLQEVFRMRGFAVKRFPKWETEQM
                     PKDEVMNLERELREAGILGQ"
     gene            12845..13921
                     /locus_tag="VF182_16675"
     CDS             12845..13921
                     /locus_tag="VF182_16675"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438774.1"
                     /translation="MNEIVISARAKPGSNFSRRRLNHPAIRAVATKVRNILCLISIAT
                     LAQASLFFSPVHAEPVRLAYSAISGAMAPLWVAHDYGYFRRQGLDVQLLYIGGGAVVT
                     QALIGGDVQFVRLGANSVVQASLRGAGIKMIGNTINTLVFSLMARPEIQTANALKGKK
                     IGVTRLGGSTDFALDLALKQWGMRRGSDVAVIQTGGMPQLLAAITGGIVDAGVVSPPT
                     NLTAAKLGLKELVDFGEIGIVYPNSPLATSQQFLDKNRSTALRVLRAYSEGIHRVRTD
                     KDATMKVLAKYTKVNDPEILAELYRLYGIKYLEPIPRVRLDAVEEVLRSEVKTATGAK
                     ASDFVDNSLVAELEQQGLFQALYR"
     gene            13931..>14609
                     /locus_tag="VF182_16680"
     CDS             13931..>14609
                     /locus_tag="VF182_16680"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6438775.1"
                     /translation="MAYQDLRQWLDQVNQLGELKNVEGADWDLEIGAIIEMARKESQV
                     APCLLFDSIKGFAKGLRIVAGQLNSIKRVALTCGMPTDMATMDLVRIMRKKIKDLKPI
                     PPREVKEGPFMENVWEEKDVDVLRFPTPRYNIGDGGRYIGTGHLTITRDPEEGWLYIG
                     TYRVVVHDSKSLGLYISPGRHARIQMEKYFAQKRPMPVALSLGHDPLLFMASAFEVPY
                     GLSEYDFA"
BASE COUNT         3414 a         3699 c         4070 g         3426 t
ORIGIN      
        1 cacggttgca cggcgaatgg ccgagctgat cggcggtaca attcaggtca aaagtgagcc
       61 gggtatcggc acgacagtca tgatgagatt ccctcctaaa gctgccgcat cgtccgcagt
      121 gccgctggag cagcggaatt atggctcgcg ataaattcct ttggtgtcgc aagtgcgacg
      181 ctgttcatca cgtgacatcc tgggatagag ctcctcaata taactacaat aactgcattg
      241 acggcgaact cgatgagata cccgccgacg actggcgtgc tttcatgaaa gaacatgaag
      301 gtcaccggtt agagccatta gaagcatcag gcgaaaagta cttttgccag ggaccaatgt
      361 ctgatcctat gggtgtcgct tacattgagg tcaccaatgg gcgcgaccat cttctcttgc
      421 gtcaatcgcg aaagagtatc gctgaaccgt taaggtttga gctaacccgc gaccggttaa
      481 ttgatcaagg ggtgacggtt gggattcaag acagtgaaat aaaaaaagag atgagacacc
      541 actttaaatg gtcgggtggt ccgttcgaag atcagaagat tgacctgttc attcagctat
      601 tgaggaatgt ggtcaccgat ttagaaccac gggcgattga ggtggctggg ttttgttccg
      661 aagaccacaa cgtcagtttt ggctcgttac aggctgaggt catcgagtcg ctcatggccg
      721 aatgcgcgct gtgtttccag ccgcaagaat tggacgacat acggcgattt atcgattctc
      781 accgtgaacc ctccgatgtt ctggccttgg ttatgcgccg acagttgaag gtcgaacagt
      841 ctgctgccgc tattaagtag cttccactat ttcggcaatt cagtcgagcc ttattcgcaa
      901 ttagttaagc atccccttcc atcaacttga cggtcgagcg accgcctgaa cttgcatccg
      961 tgcttaggag ccatagcgcc ttttagcgaa ttcatttctt aaccaccact tcatctctgc
     1021 cgtccacttc tttaagcttg agccgaacct cgccgtcttg cggtaaaata atttttctcg
     1081 ccacaaaatt agcggcaatc ggaaactcgc gtccgccacg gtctatgagc acggctaaat
     1141 ggatggctct gggacgtcct aagtcgatca agtgatccac tgccgcgcgt accgtccggc
     1201 cggtgtggag cacgtcgtcg aatagaacga tcgttttgtt attgatatcg aaaggaatgc
     1261 tcgtgtgatc gacctttcct tccggaagca gcgtgttaag gtcgtctcta tataagctta
     1321 tgtccatttc gcctagagga atgtctttgt gctgggactt ctggattatg cctcgtactc
     1381 gctgcgccaa aaaaaggcca cgagtgcgga tccctataaa ggccaaagaa tccgaagctc
     1441 tattctgtcg gagaatttca cgagccatgc gctcgagggt tcttttgatt tcggacgctg
     1501 tcaaaagtct gacgttttta gattttttca ttcgaggctc gcacgatagc gtctaaaagt
     1561 aattgtgcgc ccagacagat gtcttgggga gcagaccatt ccaacggatt atggctaatg
     1621 ccccggcgac tgggaataaa caacatcccg gctggagtaa tttttgccat ttgcattgcg
     1681 tcatgtcccg cgcctgaagg cataatctca taagcaacct ctcgaatctc gcagcagtct
     1741 tgtagcagat ggacgatgcg cttatccagt ggcacagggt cctcttttct tattggcagg
     1801 atcttaattc ttatgctgcg gcgccgagcg atctcgcgaa ttttagcttc gatcaaacgg
     1861 accacccggt tcttggctga cgcggtgata ctccgtacgt ccaccgccaa ttctgctttg
     1921 cccggcacgg cgttgatcac tccaggctcg attctaagcg cacccacagt accgactacc
     1981 cggcccttgt cgatatccga atgctggcga caaatccctt ctatggctac ggttaattca
     2041 gctgctgcaa ccaaagcgtc ctttcgcatc tccattggag ttgtgcctga gtgatcggcc
     2101 tgaccggtga tgatgacctt gaaccgtgtc ggtgcggcaa tcgatgtcac gacgccgata
     2161 ggttttttcc tcgcttccag gatcgggcct tgctcgatgt gcagctcaag ataggccttc
     2221 aacgatgatg gatctcgacg cagcgaccgg aggttgtttc gatgaatgcc gaggccagcc
     2281 aagatatcct ctagttttgc tccttggggg tcgctcgcgt ttgcaagatc gtcggcgcgc
     2341 acctcgccgg caaccaagct gctcccgagc gttgaaaaac caaaacggct cgattcttcg
     2401 ccgacgaagc aaacgacctc aaccggcgag cgcagcgcaa cattctgttc tttcagtgtc
     2461 cgaactgctt caagggcggc aatgacaccc attgtgccat cgaattttcc accatggacg
     2521 acggtgtcta ggtgcgagcc ggcaagcaca gcgggcgccc gtggatgagg accgtcgagt
     2581 ctgccgaaaa tgttgccaac gcgatcgatg ttgatctgca agccaatttg ccgcatttgg
     2641 tgaatcagga attgacggct acgcaattct ttgatcgaaa agaccagacg agtgacggag
     2701 ccgtgatcgc ccaaaccgat tctggaaacc gcgttcaagt cgcgcaacag ccggcgtttg
     2761 ttgatggtga tatcggtttg gctccgaatg gcgttcgggg tttgcgactt catgcgcatg
     2821 atcttagcag cttcgcgcgg tgctgaaaaa ctacattgtc agtgtaaaag taagttggaa
     2881 agaggcgaat gcgtatgcta tagctgcata tccaaaattg aaaggtcggc gccatgagtg
     2941 aagtttccaa gaaagtagcc gagatgctgc tctccacaga gtccatccaa gtttacaaag
     3001 aaactccctt tgtcttcgtt tccggacgca tctctccggt ctatattgat tgtcgcaagc
     3061 ttttatcctt cccgatggaa cgtgaattca tcgttgctgc attggccaaa atggttgaag
     3121 ccgagatagg gaccgacaaa atcgatgtcg tcgcgggagg cgagacggca ggcattccgt
     3181 acgcttcgtt tgttgctcat atgatcagaa agccgatgat ctatattcgc aaacagccga
     3241 aagggtacgg cgggactaag cagatagaag gcatcctcaa tccaggaaaa aaagtactac
     3301 tggtggaaga tttggtcact gatggactga gcaagcttcg tttcaatatc gggatacgag
     3361 gcgccggggc aaaaatgacg cactgcctct gtgtattcga ttacgcttcc gaccggctta
     3421 accaacatga gggcaaggaa aacctggcaa aaaatgatat ttcgctgcac gtgctcgcaa
     3481 attgggatga cgtgctggac accggattgg ctaaaagcta ttttagtgaa accgccagta
     3541 agcacatcac agatttcttg caggatcctg agaattgggg tcgcaaaatg ggtttcgtgt
     3601 gaatctattt acaatcctac gtcgccgatt gcgcaaccga agttacttat ccgtaatcca
     3661 ggttgcggtt ttcggatgcg gaactaactt ttacgattgt tgaaggacat tgttcaaggt
     3721 gttaaaaaaa atcttcggtc gcatagctca gttggttaga gcgctcgcct cacatgcgag
     3781 aggtcggggg ttcaagtccc tctgcgacca ccacctgctg tgataccggt ggttatcggc
     3841 cggtcaagag actaatcaga atatacgttt gggagatctc aactgtccat tgcgccgctg
     3901 acttcattgc ggtactcgat atctccgcct cgaatagctc ctcaacctcc aaggctctgc
     3961 taaagtcaat aacagaatgc ggctcgatta tgccgcttgc ttttggttct ggcgcagatc
     4021 atcgaggttg ttatcgaaat tttcctgctt tcgatgaacc gatctctgtt gtgccttttt
     4081 ccctcaatcc tgctgcaagg catcggtgat cggctctggc gtctgaacct tgggaattgg
     4141 aaggtgatca tcgcgagagc gcgattcagg agtttagaag caagcttgtt cggttatctc
     4201 catcactgta gatccgttgt aggaggggtt ctatctggat ccagggttca gttgcgcaag
     4261 tgaggcggag cgagtcagaa agttgccatc gcatcaggcg ggtggactag gtttgagccg
     4321 catggcggca ttagagttac agaaagcgga tcattctacc atcttgaccc atggtctcat
     4381 gaaggaaaga tcgaacgggg gtattttccg gagcgaactc gacgtaggcc gaatacgtac
     4441 gatggtcagc cttgctaggt tatctaactt gaagaggcga ttttaataga cctaaccttg
     4501 tcatctgagt cttcatgacc cggcatgtgc taccatagtt tcgctgtgca acttctaaga
     4561 tggcagctgc cccctcctca tcgcagggga tcgtaacaag ttgaggatga ttagttttgg
     4621 gttgggccat tttgagaacc gagctcgcag gtgatttgcg cgaagccctg gcaagtaaac
     4681 gttctttctc ttcgtacttc aagataattt ctgcatgcat aggatctcct caagtcatca
     4741 gtcgagcact tgaagttgcc agcccgtaat tcgcgacgac actccgcatc tagttggtag
     4801 aatgatttac tcgcgatcta gttagagccc gataaaacgc atataaagca gcgctctgtt
     4861 cccgggaagt ttggatacgt tggttgatga tcgtccgtat gatcctagtg cgcttgtgat
     4921 cctctctctc gtaacgctgc atgactgtaa cgtaagttcg ctcggcaatc gatctattct
     4981 tcatacttga gcctcatacc agatgtgcgg gtttcgaacg gaaacttgat cagtgcgacg
     5041 gatgattcgt ttgtgactca aaataagaca caaaccctgt cgctaagcaa tgaccaggcc
     5101 cggaacatta tggataacgg ccgatatctt aagtattttt gagatatgtc ctagtcccgc
     5161 gcgtgggaat agcgcattgt agggtgtagc ttaaatgaaa cagaacctac cgttttttaa
     5221 gatcggcgtg cgctcactat aaagctgtcg gttcggcgaa tcagagcaac ttcttcgaga
     5281 tcgacaaaaa aatgcggacc taggcccgtg gtccaaatcg gcgttatccg aaccgaccat
     5341 cgagattgca ttggcatggc tcctggccag aatatttaac ttaacgacag cgtacgcggc
     5401 gcggaagcgt gcgaagctcg gcagatagaa ataacgcagg cacgcgggag ccgccagaga
     5461 aattttcccg ctgaaatgag ccgcatgatt tttggttttt tccttcgcct cttcgactga
     5521 gttgagcacc tttctggctt cagcatagaa gatttcaccc tggggcgtga gttccagttt
     5581 gcgtccctat gatcgaaaag cttcagagcg agctcctgtt cgagtgcctt gatctgcaga
     5641 gataaggccg acggagtgag aaaacagcgt ttcgcggcat cttttaacga gccgctctgc
     5701 acgatccaac aaaaactatt tagttgtctg atctccatag aaaatacttt agaaaaatta
     5761 aactgatcat taagtaaatc agattgacat gagttcaacc gcctgcatag atgacgatgg
     5821 ctcgagaatc atgaatagat cgcgaaaagt attgttgagt gacctgtgcc gcgtcgccgg
     5881 cggcactcct gcgggcgatt ggttccgctg ctattggctg gcggtgggca cggccggtga
     5941 actgcgcgac gtcccgcgag ctgtcagagt gctgggtgag gaactggttt tatttcgcga
     6001 cgatcgcggc aggattggct tacttggcct tcactgcccg caccggggaa gctctctcga
     6061 gtatggcgat atcgagaacg gcgggattcg ctgcccctat cacggttggt tgttcgatgt
     6121 ttccggtcag tgcctggagc aacccgcgga acctgaggga agtatttttt gccgcaaggt
     6181 aaagcatctc tcctatcctg ttgcagaact cggtggattg attttcgcct atatgggtcc
     6241 cggccaggag agcccgccgc cattgccgca gtataaagct ttggtcgatc caaatgggca
     6301 gagatcgtta gaagcaaccc gcgagtatga ttataattgg ttcaatttca tcgagaatgg
     6361 cgccgacccg gcgcactttt cgatcctcca tcggaccgat cccaatgacg gcacctggcg
     6421 cagctggttt tttaacttca aagacgttcc gccattcgat gcggtggaaa cttcctacgg
     6481 catgaaggtg atctcgcgca aaccgggacc agccgccgat actgagtacg tcgatgagaa
     6541 gagcttcgct ctgccgagca ttttgcaaat cggcgatact gaattcaccc attttagaaa
     6601 acccaaagag gcgctggccg caggctcgca caacgcgcat tttatgtttg tgacgcccag
     6661 cgacgatgat catttcacgc tgttcaccgt caaccattac accgggcccg atccggaatt
     6721 ttttcacaag ctcgagccga gccggcgcat tgtgccgaag atggaaaaga aagaatacga
     6781 tcagcgccgg tactcgcctt ttcgcggcag cgtccgcagc gaagacatta tgagccaatc
     6841 aacgcaggga accgtcgccg agcgtaaaga gcagcttgca acttcggaca agggcgtcat
     6901 cctgctgcga aagatcattc tcggggcgat cgaaacgatg cagaaagggg aagttccaaa
     6961 aggggttcct ctgcgcgaag attctgccaa ggtgatcgat atcgactcgt tcaccggcat
     7021 tagacctaaa ggagtctttt gatttgaaac tctcggtaat aatgtcggcg cggtttctga
     7081 cctcagtgtt tctgggatta tttttggttg ccagtgcggt cgaagcagcg ccgctgcgca
     7141 ttgcctatag cgctatcagc ggcgccatgt cgtcgctctg ggtcgcccaa gaggcaggct
     7201 acttcaaacg cgaggggctg gatgtcgagt tgctttacgt cggtggcggc tcgctgttga
     7261 tccaatcgat gatcagcggt gatgtgccgt ttgcctatgg accttcagtg ccggtgatta
     7321 atgcttcact tcgtggcgcc gatcttgttc taatcggcaa caccagcaat tctttggttt
     7381 tctcgattat ctcccggcca gaaatcaaac agtcggcgaa tcttaaaggc aaaaaagtcg
     7441 gtgtgacccg ccttggcggc tccacggatt gggccctcga cgcggcattg aaacagtggg
     7501 ggctggagcg gcgcgagatt actgtgattc aaaccggggg tatgcctgaa gggctggctg
     7561 gtctgatggc aggcgtcttc gatgctgtgg tgctgtcgcc gccgagcaat ttcagagcgg
     7621 tgaaagccgg gatgcacgaa ttggtcgacg tcggccaatt gagaattatt tttcccaaca
     7681 ccccgctctc gacgacccag tctttgatca aaggaaatcg ggatagcgca ctacgcttta
     7741 tgcgcggttt cacccaggga ctacatcggc tgcgcaacga taaagagttc agtatgaaag
     7801 ttctctccaa atataccaag gtcaccgaca atgaaacgtt ggcgcaactt catcaaactt
     7861 acggcgtgcg ctacagcggt gatcgcatcc cttacgtgcg cacggaaggc gtggatgaaa
     7921 ttctcaagcg cacgccagga aaagaagcgc gcgaagccaa ggccggcgac tttgtcgaca
     7981 acaccttgtt gagagatctc gaacagagcg gctggttcaa aatgttggag cgttgattca
     8041 tgcgggtcgt cgtcacagga ggttccggac gcttgggaca gtacgttgtc cgggaactgt
     8101 ttactcataa tcatgcggtg gccgctctcg atgcgataaa accgcgcgaa tgtttatgcc
     8161 cgacctatac ggtcgatctg atgaggatcg actcgtttgt cgatcatctt aataatgccg
     8221 atgccctggt gcatcttgcc cgggtgcgct ttccttatac ggagaatggc tacaacgtgg
     8281 ccgagcagaa gtgggagttc gccgatattg caggggatgc cgaacgtttc aatcgcaatg
     8341 tcgcgatgac gaacaatgtc cttgctgcag cgcaagcgtg cgcaatcaag aaagccgttt
     8401 gtggatcgag cctggcagtg tatggctttt attatccttc cactgggctg cagcctgagc
     8461 atttgccggt cgatgaagat catcctttgc ggccgcaaga tccttacggc atcagcaagc
     8521 tcatcgtcga aaaactctgt gatgccttga gccataagac cggcgctcag gtcgcaagtc
     8581 ttagatttgc cggaatctat accgaggcgc atcggtcgat gttgctcgac cgcaagaaaa
     8641 atcctctcgc gcgaggaacg ggagcgctgt ggagctatat cgatgcgcgc gacgcggcgc
     8701 gcgcctgccg gctggctctc gagaccgatt tcggcggcca tcaagctttt aacatctgcg
     8761 cgtcttcgac catcatggac acacccaccc gggagttaat cgcgcgctac ttgccgcgag
     8821 taaaagttct gcgcagcggc ctggatgatc gcgccagcgg ttacagtatc gagaaggcaa
     8881 agaagttctt gagttttgag ccgcgttgtt cgttagtaaa ctgatatcgt aggggtgggt
     8941 tttaagcccg cccctacctc ctctaggcag tctgcactgg cgcgccaatg ctgaatgcat
     9001 gcaagggaat tagatttttc agtcatcaag agtcgacggg agccattttg acatgatgga
     9061 tttgagagaa ttcttaaagg tcttggaaga agagcacgag ctacaaaaaa tcaaagtcga
     9121 agtcgatccc aagcacgagc tcggagcgat ctgtaagatc cacaacgagc ggtcgaacag
     9181 cccggcgctg ctgtttgaaa acgtcaaagg tcatacgatt ccggtggttg gccaattgct
     9241 ggcgagcgac cggcgggtag cgttggcgct tgggctatcc caggaaaatg tgtttaacga
     9301 aaccgtgcaa cgggcgagca atccgattgc accgaagttg gtctctaaag gcgcctccca
     9361 ggacatagta ttggaaggcg ccggcctcga tcttacaaaa ttgccgcttt gcaccaacaa
     9421 tccgcgcgat ggcgggccct atatcaccgc cggtcatgtg atcatcaaag accctgagta
     9481 tggcatgaat ctttcgatct accggatgat gctcgtgtcg aaaaacgaag tcacccttcg
     9541 ttttactcct ggccatgacg gctatgattt tatgaaaaac gccgagaagc gcggccagag
     9601 gaaatttgaa gtcgcggttt gcatcggtgt gccgccggcg gtttatgtgg cgtctcagtt
     9661 cgaaccgcgc ctcggcgtct atgaattgga cattgccggc ggccttgtcg gcgagcccgt
     9721 cgaggtggtg aagtgccgga ccatcgatct cgaagtgccg gcgcttgcgg aaatcgtact
     9781 cgaaggggaa ctgacgatcc cggcgaaaac cggcgacgag gggccgttcg gcgagttttg
     9841 cggctatacc accgcgcaag tgccgaacga gcgcatcatg actgtcaaag ccgtgacgca
     9901 tcgcaaaaat ccggtctacc acaacatctg gctcggcaag ccgccgcacg agcatctcta
     9961 tgtcgatgcg ttaacctatg cggtcgcggc ttaccaggag ttgaagccgg cttatccggc
    10021 attgaagaaa gcctacgcgc cgccctgggg cgtttcgatt gtgttgctgt tgcagcttga
    10081 gaagcggctc atgagaccgg gcatcgtcga taacattctt gccgcttcgc tgtacacgcg
    10141 aagcgggaag tggaaacatg tgtttgttct cgacgaagat atcgtccttg aagatcccaa
    10201 cgaagtgctg tgggcgctga cgacccgttt ccagcccgct accgatatgt tcattatccc
    10261 gcgcggcatc accagctcgc tcgaaccgtc agccacggtc gatggattga cttcgaaact
    10321 gatgctcgat ctgaccatca agaaaaattt tcgcggcgaa gtggcggagc cgaccgcatc
    10381 tatgcgagag agtgtcttaa aaagatggaa ggaatacgga ttcaaataat atccgggaac
    10441 gttcaagacg ttcaaaaagt ttaaatcgtt caagccgtaa gagaaagaac tgtaacttag
    10501 gctgagaggg aatttcagtg gttaaagcag ctgcgaaaag aaagatgaag aaggtaacgc
    10561 cggcggacat tccgcgggtt gggccgggga cgccggcggg tgagtggttt cgcaaatact
    10621 ggttggtagt cgggaccact caggagctgc acgacattcc gcaggcggta aaagtccttg
    10681 gcgaggatct ggtgctgttt cgcgacgaat tcggcaagct cggcctgctt ggcttgcact
    10741 gctcgcatcg tggatcatcg ttggaatatg gcgacatcga agatggcggg attcgctgtc
    10801 cgtatcatgg ctggttgttt aacgtcgccg gtcagtgctt ggaaatgccg gcggaaccca
    10861 aggacagcaa atttccacag aaggtgaagc acttgtccta tccggtgcgc gagcaaggcg
    10921 gcttgatctt cacctacatg ggggaaaatc aaaatgatcc gccgccactg ccaaaatatt
    10981 cgccgctggt ggaccgcggc agccagtggc agatcgagcc ggtgcgtcat gccgactaca
    11041 actggttcaa ctttttcgaa aactcggcgg atcccgccca cgtctgtatt cttcaccgga
    11101 atgccggtta tggccagcaa tcctggggca atcagttttt tagttacacg gacatgccgg
    11161 acttcgaatt cgtcgagatg gattacggca tgaaagtcgt catgacgaag cccggcccgg
    11221 aaccgggcac ggaattcgtc gatgaaatga gtctggcgtt accgagtatc gtccaggttg
    11281 gcgataccga gttcgtccat gccaagatcg attcggcggc gctggttaag gaaggttcgc
    11341 agtgcgagca ttggatgttt gtcacgccta atgacgacga ccattttatg cttttcaccg
    11401 ccgacaatta ccgcggtccg gaggagaact ttttcgaaaa acttaaagcg ctacgcgcca
    11461 aagagatgcc cgcgcaagaa gtaaagccgt acgacaagcg cccgttcatg ccgtataaag
    11521 ggaacatccg taaggaggat ctggtcactc agggtacgca gggtttgctc ggtgaacgaa
    11581 gcgaacagct cggcgtgtcc gatcgcggtg taatcaagtt tcgcaagatc gtggtggatg
    11641 ccatcgagac ggcgctaaac ggcggcacgc ctaagggcat tgtcgataaa aaggaagaga
    11701 gtgaagtgta tcggttggat acacaggtgg gagtgcgagc caaggcatca aactaagtta
    11761 gccacggaag ctcctgatga gaattaaaat caaagcaaaa tgatggtcgc ggagtttaac
    11821 ctgagcatag ccgaaggact cctcagaatg acgtgcgctg ggagccgtcg ttcctagggt
    11881 aggaacggat ttcagacaag gagaaatgaa tgattcgtgg agttttgttg ccggttatca
    11941 cgccgttcga cgaaaaaatt cgggtcgatg aacagatgat gcggcagttg gtcgattttc
    12001 acatcaaagc cggcgttcag ggattgttcg tgcttggctc taccggtcag ggccccgcca
    12061 tgaccatcga agagcgcaag cagacggccg ccgtcgcgct cgatcaggcg aagtcccggg
    12121 tgccggtgat cattcacgtc ggcaccgccg atgcgggcag tgcggtggag ttggccgagc
    12181 atgccgccgc tcacaaagcc gatgcagtgg ccatcgtgcc gccctattac tattccgatc
    12241 acacggaata cgagatcatc gcgcactaca aagccgtgca caaagccgtg ccgctgccga
    12301 tcttcatcta cgaaaatccc aagtactccg gcatctctat tccgccgggc ttagccgtgc
    12361 gcatgaaaga gcaggtgccg gcgctagaag gcatcaaagt cgcctacggt cagggcgcgt
    12421 tgctcgaata cgtgagacta ctgccggata tatcggtttt taccggaaac gcggatctat
    12481 ttggactggt tccgttcggt gttgccggta tgatcaatcc gccgacgagc ttcgtgcccg
    12541 aactctgcgt cgatttgttc aaggctctcg acagccaaga ctacccgcgc gccgtggcag
    12601 cgcagaagcg agtcgatacc gcggcgcgaa tcgtcgcagc gcgcttgcgc aagtttggcc
    12661 gagttccgtt gcaggaagtt tttcgcatgc gcggcttcgc ggtaaaacgc ttcccgaagt
    12721 gggagaccga gcagatgccc aaggacgagg tcatgaacct ggagcgcgag ttgagggagg
    12781 ccggcattct cggccagtga acgcaaattc ttaaactctc gccgatcgcc atccggttta
    12841 gcctatgaac gaaatcgtga tttccgctag agccaaaccc ggctcaaact tcagtcgtcg
    12901 ccgtctaaac cacccagcca tccgagccgt ggcaaccaag gttcgaaaca ttctttgtct
    12961 catctcgatc gcgacactgg cgcaggccag tttatttttc tcaccagtcc atgcggagcc
    13021 tgttcgacta gcttacagcg cgatcagcgg cgcgatggcg ccgttatggg tggcacatga
    13081 ttacggctac ttccgccgcc agggattgga tgtacagctt ctgtatatcg gcggcggagc
    13141 cgtggtgact caggcgttga tcggcggcga cgtgcaattc gttcgcctcg gcgccaattc
    13201 cgtggtccag gcttcgctca gaggagcggg cattaagatg atcggcaata ccattaacac
    13261 actggtgttc tcgctcatgg cgcgcccgga aattcagacg gccaatgcct tgaaaggaaa
    13321 aaagatcggc gtgacccgtc tcggcggctc gacggatttc gctttggacc tagcgctcaa
    13381 acaatgggga atgcggcgcg gttccgacgt tgcagtcatt cagaccggcg gcatgccgca
    13441 actgcttgcg gcgatcaccg gcggcattgt cgatgccggg gtggtgtcgc cgccgaccaa
    13501 cttgactgcc gcgaagcttg gcctcaagga gctggtcgat ttcggagaaa tcggcatcgt
    13561 ttaccccaat tcgccgcttg caacatccca acagttcttg gacaagaatc ggagcacggc
    13621 gttgcgagtg ttgcgcgctt actcagaagg cattcatcga gtcaggaccg acaaagacgc
    13681 aacgatgaag gtgcttgcaa aatatacgaa agtgaatgat ccggagattc tcgccgagct
    13741 ttaccgactc tacggcatca agtacctcga gcccattcca agagtgaggc ttgatgccgt
    13801 cgaagaagtg ctgcgctcag aggtgaaaac tgcgacgggc gccaaggcga gtgattttgt
    13861 cgataatagt ttagtggcgg agcttgagca gcagggatta ttccaagcat tgtaccgata
    13921 gggggaagcg atggcgtacc aagatttgcg gcagtggctg gatcaagtca atcagttggg
    13981 cgaattgaaa aacgtggaag gcgcagattg ggacttggaa atcggcgcga tcatcgagat
    14041 ggcgcgcaag gagtctcagg tggctccctg cctgcttttc gattccatca aaggctttgc
    14101 caagggtttg aggatcgtgg ctggccagct caactcgatc aagcgcgttg cgcttacctg
    14161 cggtatgccg acggacatgg cgaccatgga tctagtgcgt atcatgcgca agaagatcaa
    14221 ggacctgaag ccgattccgc cgcgagaggt gaaagaagga cctttcatgg agaatgtctg
    14281 ggaagaaaaa gatgtcgacg ttctgcgctt tccgacgccg cgctataaca tcggcgacgg
    14341 cggccgttac atcggtaccg gccatctgac gatcacgaga gatccggaag agggttggct
    14401 ctacatcgga acttaccgag ttgtggttca tgacagcaag tcgttgggac tctacatttc
    14461 gccgggacgg catgcgcgca tccagatgga aaaatatttt gcgcaaaaac gtcctatgcc
    14521 ggtagcgctt tccttgggcc acgacccgct tctttttatg gcgtccgcgt tcgaagttcc
    14581 ctatggcctt tcggaatatg atttcgccg
//
LOCUS       DASUVS010000308         3097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_345451, whole genome shotgun sequence.
ACCESSION   DASUVS010000308 DASUVS010000000
VERSION     DASUVS010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345451"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..630
                     /locus_tag="VF182_16685"
     CDS             268..630
                     /locus_tag="VF182_16685"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX6438776.1"
                     /translation="MKQEITILALCTMLFALCSSALAQRARLAQLATIHRIPAIYYHE
                     AFTESGDLLAYGPKLDEIGWNRAALFVEKILKGIKPADLPIEQPTKFELVINLKAAKQ
                     IGLTIPPNVLARADRVIK"
     gene            680..1726
                     /locus_tag="VF182_16690"
     CDS             680..1726
                     /locus_tag="VF182_16690"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438777.1"
                     /translation="MNAFSSRFPSDDRKSAIQNPKLVGIVTIVLTLVFGGAVGEAQQM
                     NKIFRIGFLDSSTASGSAVLMAAFRQELSKLGWIEGRNIAIEYRFTESKGIERLPELA
                     ADLVRLKVDLIVATDTPSAMAAKNSTQAIPIVMANVGDPVGSGLVSSLARPGGNVTGN
                     ASLTPELNSKRLEVLKDVVPRLARVGVLRGSRSGGSGEVQFKELRPAAVALKLTLEEI
                     KTQPDLKGLESAFQTAKQKQVDAIMTQSTRPFFAERKRIVELAVKYRLPAIYFQDDFV
                     EAGGLMSYGADFEDLYRRAAVYVDKILKSAKPGDLPVQQATKFEFVINLKAAKQIELT
                     IPPRVLERANKVIK"
     gene            1778..2761
                     /locus_tag="VF182_16695"
     CDS             1778..2761
                     /locus_tag="VF182_16695"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438778.1"
                     /translation="MNEKIFCVVMCGLFLALTFSAQAQQPRVYSVGLVSLGNNVTAEI
                     SGIRDGLKDAGYIEGKNLLLDVGIAKSYDGLRPIIQSYIGKRFDIIVSTGASAPLIAK
                     ESTQDIPIVFIGGADPIQAGLVKSMARPEGNVTGIARYRDVEIYGKRLEVFKEAMPSL
                     RRVTVIYNARGENPTHFASLRLLQKLAPKLGITLIEKPIKTVRNMEKALSEISKDTMD
                     GLFPICATIFRDLTKNMAETAGQKKVALFGCSPVVVTEFGALLYYGANNYRVGQRGAW
                     YIDRILKGSKPQDLPVEIPSYFEMIVNLKTAKQIGLTIPPTVLGRADRVIK"
     gene            2882..>3097
                     /locus_tag="VF182_16700"
     CDS             2882..>3097
                     /locus_tag="VF182_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438779.1"
                     /translation="MKSFLAAILTVLVATSIGLAAGESAGKIPRIGILFIGGRDQPHL
                     ETFKQGLRERGYVEGKNIVLDYRYAQGK"
BASE COUNT          809 a          755 c          833 g          700 t
ORIGIN      
        1 aacgacccga tcccggtcgg tgaccagcac cgtctcgccg ccttcggcga gccgaacata
       61 ctcgctgagt ttattcttta agatctttaa accgaccgcc ttcatacgca tcaatgtagc
      121 ttccggtagc taccgatgtc aaggacggat gtttaaacga gcccgttgcg tcggaacctg
      181 cctttttcgg ttccgcgaag gtgcggagaa gatgatcgat tgacgcgagg aagtgatgag
      241 tgcgcagcgg aaagagcaga gaaaaacatg aagcaagaaa ttactattct tgcgctttgc
      301 accatgctct ttgcgctttg ctcgtcggcg ttggcgcaac gcgcgcgcct ggctcagctc
      361 gccacaatcc accgaatacc agccatttac tatcacgaag catttaccga gtcaggcgat
      421 ctcctggcat acggtccaaa actcgatgag ataggctgga accgcgccgc attgttcgtt
      481 gagaagatct tgaagggaat caagccggcg gatctgccga tcgagcagcc gaccaagttt
      541 gagctggtga tcaacttgaa agccgccaag caaatcggcc tcacgattcc gccgaacgta
      601 cttgcgagag cggacagagt cattaaatga ttttggattg tcgattttag attttgtatt
      661 gaaagcagcc gacgaattta tgaatgcgtt ttcttcgagg tttccatccg atgatcgaaa
      721 atcggcaatc caaaatccaa aattggtggg gattgtcacg atcgtcctca cgttagtctt
      781 tggtggcgct gtgggcgagg cgcagcagat gaacaaaatc ttccgcatcg gtttcctcga
      841 ttcaagtacc gcttccggta gcgcggtcct catggcggcg ttccgacaag agttaagcaa
      901 gctcggatgg atcgagggaa gaaacatcgc catcgaatat cgatttactg agagcaaagg
      961 cattgagcgg ctgcctgagc ttgccgcgga cttggttcgt ctcaaggttg atctgatcgt
     1021 ggccacagat acaccgtctg cgatggcggc caagaactcc actcaggcta tccctatcgt
     1081 catggcgaac gttggggacc ccgttggttc aggtttggtt agcagtctcg cgaggccggg
     1141 cggcaacgtt accggaaacg caagtttaac gcctgagcta aatagtaaaa ggctggaggt
     1201 actcaaggat gtagttccca ggctcgcgcg agttggagtg ctgcgcggtt cgagaagcgg
     1261 cggatccgga gaggtccaat ttaaagaact cagacctgcg gctgtggcac tgaagctcac
     1321 attagaggaa atcaagactc aacccgacct aaaaggttta gagagcgcgt ttcagaccgc
     1381 aaagcagaag caggtcgacg caattatgac gcagagcact cgcccttttt tcgctgaaag
     1441 aaagcgcatc gtcgagcttg ccgtcaaata ccggttgccg gctatttact ttcaggatga
     1501 ctttgtggaa gccggcggtc tcatgtcgta cggggcggac ttcgaagacc tctatcggcg
     1561 ggcggctgtt tacgtcgaca agattctcaa gagcgcaaag cccggcgatc tacccgtaca
     1621 gcaggcaacg aagttcgaat tcgtcatcaa cctcaaagca gcaaaacaaa tcgagctgac
     1681 cattccaccg cgggtgctcg agcgggcgaa taaggtaatt aagtaagaaa tcatcactgg
     1741 caagtaacca gggctccgca agacaaagac aattgtcatg aacgaaaaaa tcttttgtgt
     1801 tgttatgtgt ggtctattct tagcgcttac attttccgcg caggcgcagc aacccagagt
     1861 ctattctgta ggactcgtgt cgctcggaaa caatgttact gcagagatca gcgggatacg
     1921 cgatggactg aaggatgcgg gatatatcga ggggaaaaac ttgttacttg atgttggaat
     1981 tgcaaaaagt tacgacggcc tgcgtcccat aattcagtcc tacataggaa agcgattcga
     2041 catcatcgtc agcaccggtg cctcggcgcc actcattgcc aaggaatcca cacaagatat
     2101 ccctatcgtg ttcatcggcg gtgccgaccc gattcaagca gggcttgtta aatcaatggc
     2161 acgcccagaa ggaaatgtca ctggaatcgc gcgctaccga gacgttgaaa tctacggcaa
     2221 gcggttggag gttttcaagg aagctatgcc gagtttacga agagtgacag tcatttataa
     2281 tgcgcgcggc gaaaatccga cgcattttgc tagtctccga ttacttcaaa aattagcacc
     2341 gaagctgggc ataaccctaa tcgaaaagcc gatcaaaacg gttcgtaata tggaaaaagc
     2401 cctgtcggaa atctcgaaag acacgatgga tggtttgttt ccgatatgcg ccacgatttt
     2461 cagggacttg actaaaaaca tggctgaaac ggcgggtcaa aaaaaggtcg cattgttcgg
     2521 ctgtagccct gttgtagtaa ccgagttcgg ggctctttta tattatggcg cgaataatta
     2581 ccgagtggga cagcgaggcg cttggtacat agatcgaatt ctcaagggat cgaaaccgca
     2641 agatcttccg gttgaaatcc cgagttactt cgaaatgatc gttaatctca aaacagcgaa
     2701 gcaaattggt ctgactatcc cgccaaccgt gctaggaagg gcggacagag tgatcaaata
     2761 aaagggcgct aagcggctga cgtctttgcg tcgaaccaca acaccggaga gttccgcgga
     2821 ttggttgtct gattaatgac tccgctctga ctgggccacg gcgctccgcc gttgcgatag
     2881 aatgaaaagc ttcctagctg cgatactcac cgttcttgtc gcgacctcta tcggcctggc
     2941 agcaggtgaa tcagcaggta agattccgcg catcggaata ctttttatcg gcggacgtga
     3001 tcagccacat ctcgaaacat tcaagcaagg gcttagagaa cgtggatacg tggaaggcaa
     3061 aaacattgtc ctggattacc gctatgcgca gggcaag
//
LOCUS       DASUVS010000309         6306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_783285, whole genome shotgun sequence.
ACCESSION   DASUVS010000309 DASUVS010000000
VERSION     DASUVS010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6306
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783285"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VF182_16705"
     CDS             complement(<1..285)
                     /locus_tag="VF182_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438780.1"
                     /translation="MPLAKIQTASIEMQSRGQRAKEIGPKRLEDGPVSIQEGRRSRAI
                     HVGVDLTALLPEATGVDNYLIRLVVHLSRIDQANHYSLFVNYEDLELFRGW"
     gene            complement(312..644)
                     /locus_tag="VF182_16710"
     CDS             complement(312..644)
                     /locus_tag="VF182_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438781.1"
                     /translation="MDEQNELQHVVLTRLLRLNSTIQGIISGLVAGLGIFIATNWLVL
                     KGGDVVGPHLSLLGEFFIGYEVSFAGSLIGFAYGFVTGFVVGYGVTTMYNYIVEYKER
                     TSERHTKS"
     gene            complement(659..2464)
                     /locus_tag="VF182_16715"
     CDS             complement(659..2464)
                     /locus_tag="VF182_16715"
                     /inference="COORDINATES: protein motif:HMM:NF005548.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6438782.1"
                     /translation="MNDPRPGVKQVVVIGAGPAGLTAAHELAKLDGKPIVIEKGDKVG
                     GIARTEVYKGFYFDMGGHRFFTKVEEVNKTWREVLGDNFLRRPRLSRIYYNRKFFDYP
                     LKPMNALAGLGLWQSILITLSYIRWQLRPYPEEETFEQWVTNRFGRRLFRIFFKAYTE
                     KVWGISCSELKAEWAAQRIKDLSLKTVLLNMFVTPKKTIKTLIDQFDYPQRGPGMMWN
                     AVKGEVEKRGGSVRLNSEVVAIHNGGGKYIHSVEVACNGHKEIIHGSEFISSMPITEF
                     VKKLDPPAPTEVLRAADKLRYRDFLTVCLIVNQPSLFPDNWIYIHDPAVSVGRIQNFK
                     NWSPHMVPDDTKTSLGLEYFCNEGDALWNMADADLIELGKCELDHIGLASPADIEDGC
                     VFRVPKAYPIYDSEYREYLSTVKDFVGSLENYQTIGRNGLHRYNNQDHAMVTGMLSVR
                     NIMFGEKNDLWKVNTDQEYQEEIRREINIEPEIAVDALKEAFAQAFAKLDRFALGLSI
                     GTAAGLLLFLATLALLLGGGGIAGPKLELLQQYFPGYTVSLTGSLQGLVYGFLTGFAA
                     GWIFAFLRNIALFVCTVMTQRRAERLLLRHFLEYV"
     gene            complement(2493..3557)
                     /locus_tag="VF182_16720"
     CDS             complement(2493..3557)
                     /locus_tag="VF182_16720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX6438783.1"
                     /translation="MSTEEIQVPSISVVVPAFNAALHLNRCLEAVFANDRIRECIVVD
                     DGSTDDSASIARQFGARVLHLSDGPFGPAYARNRGAEAAVGAVIFFVDADVVLAAGAF
                     DRVLTVFQEHAHLAAVFGSYDAHPAASGMISQYRNLLHHFVHQNGNTEASTFWAGCGA
                     IRRSVFTRIGGFDEKRFSRPSIEDIELGYRLRSAGYRILLDKQLQGTHLKRWSFLSLI
                     RTDVICRAVPWSRLIHETTKAPQELNLAPAQKASVALMGSACAFVALGLVWQGSFALS
                     ALALSGVVFLNRGLYAFFRRERGMSFAISCIPLHLFYNLYSGLSYFYVCAEFRIKKLT
                     KARPASKLKSASQTITVKRT"
     gene            complement(4264..5382)
                     /locus_tag="VF182_16725"
     CDS             complement(4264..5382)
                     /locus_tag="VF182_16725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX6438784.1"
                     /translation="MQKLTVVGLLILFMLLTGELLVRYLIPAWPFEPALYVPDYLTAR
                     DMPLRWRFSPSDGRNSLGLRNREVGPKKAGTSRILFLGDSLIWSGETSSGKLYTEVLE
                     QRLNARLTNSPGAVEVINAGVPGYTTYQELEFLKIYGLDMKPDLVMLGFVFNDVYYKY
                     LHKPNSQRFLGREPTAHLYHFDPEKFPGILFARSHLAHQLASMTENLWKRILGRPVFP
                     FEQRGDFYLAWQPYGWDHARALIREMRSLLRQKGVPLMVLVFPISDQVNDEYRKLDQA
                     FILYPQRRIREICEEFGIPMLDLTEALYRKGGETLFRDYLHLNGKGNDVIADVLEKYL
                     VERFKDPERSAEELILPVYPEPARSPNFDVSTAEQTFN"
     gene            complement(5527..>6306)
                     /locus_tag="VF182_16730"
     CDS             complement(5527..>6306)
                     /locus_tag="VF182_16730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX6438785.1"
                     /translation="APIKDIAVGALFRSTVIGFMGNYLLPFRAGEIMRAVSIGQTQDI
                     SKSAALGSIVLERVFDGVVISLTPFIVLAAVELPPWVTRVNIALLTLYITGLVGLVIA
                     IRHGWTELWIHKAMVLMPMGFARHVTPIATEFLHGMKGINYAGALLPVCLLSFVCWFF
                     HGLYFFLLFHALDLELSFWTALIIQMVIGLGVILPAAPGYVGNFEYFTVLALALFGVT
                     HEMAFAYALLAHICQFVSVTAVGLSFVVGTGFRRQVQPASI"
BASE COUNT         1589 a         1693 c         1575 g         1449 t
ORIGIN      
        1 ccagccacgg aacagctcca gatcctcgta attcacgaag agactatagt gattcgcctg
       61 atcaatccgg ctcaaatgca caacgagccg gattaaatag ttatcgacgc cggttgcttc
      121 gggtaatagt gctgtcaggt caacgccgac atgtatagcg cggcttctcc ggccttcctg
      181 tatggatacc ggcccatcct ctaaacgttt cgggccgatt tctttagccc tttgacctct
      241 ggattgcatc tcgatggatg ccgtttgaat ctttgcgagt ggcatgattg caaaccgatc
      301 agaacttaca tttaagattt cgtatgccgc tcgctagttc tctctttgta ttcgacgatg
      361 tagttgtaca tagtggtaac accgtatccg acaacaaagc cagtgacaaa gccgtaggca
      421 aagccaatta agctgcccgc aaagcttact tcatacccga taaagaattc tccaagcaac
      481 gaaagatgtg gaccgacgac gtcgccgccc ttgagaacca gccaatttgt ggcgatgaat
      541 attccgagtc cagcgacaag tccgctgatg ataccctgaa tagttgagtt gagtcgcaac
      601 aaccgtgtca gcacaacgtg ttgcagctcg ttttgttcat ccatgatggc cggtgctctt
      661 aaacatattc aaggaaatgg cgcagcagta agcgctctgc gcggcgctgc gtcattaccg
      721 tgcacacaaa caacgcaatg tttctcaaaa atgcaaaaat ccatcccgcg gcgaagccgg
      781 ttaggaatcc ataaaccaac ccttgcagac ttccggtcaa gctgacagtg tacccgggaa
      841 agtactgttg gagcagttcc agcttcggcc ctgctattcc tccccctccg agcaagaggg
      901 cgagtgtcgc caggaacagc aatagacccg ccgcagtacc gatggataag cccaacgcaa
      961 atcgatccag tttggcaaac gcttgagcaa aagcttcctt caacgcgtcc accgcgatct
     1021 caggctcgat attgatctct cgacgaattt cttcctggta ttcctgatca gtgtttacct
     1081 tccagagatc atttttctcg ccaaacatta tgttgcgcac gctcaacata ccggtgacca
     1141 ttgcatgatc ctgattattg tatcggtgta gcccattgcg accaatggtc tgatagtttt
     1201 ccagactgcc gacgaagtct ttaacggtgg atagatactc gcgatattcg ctatcataga
     1261 tcggatacgc ctttggaacg cgaaatacgc aaccatcttc gatatcagct ggcgatgcca
     1321 atccgatgtg gtcgagctcg cattttccga gctcgatgag atcggcgtct gccatattcc
     1381 acagcgcgtc accctcgtta cagaagtact ccagccctag gctggttttt gtgtcatcgg
     1441 gaaccatgtg agggctccaa ttcttgaagt tctggattct acccacactc accgccgggt
     1501 catgaatata gatccaattg tcaggaaaaa gactaggctg gttgactatc aagcaaacag
     1561 tcagaaagtc gcgatatcga agcttgtcgg cggctcgcag aacttccgta ggagcgggcg
     1621 gatcaagctt cttgacgaac tcggttatcg gcatgctgga aatgaattcg ctcccgtgaa
     1681 taatctcctt gtggccgttg catgctactt ctacgctgtg gatgtacttg ccgccgccat
     1741 tgtgaatcgc cactacttca ctgttgagcc gcacagatcc cccccgcttt tcgacctcac
     1801 ccttaaccgc attccacatc atcccggggc cgcgctgtgg atagtcaaat tgatcgatca
     1861 gcgttttgat ggtttttttc ggggtcacaa acatgttgag caggaccgtc tttagtgaca
     1921 ggtctttgat tcgttgagcg gcccattcgg ctttcagttc cgagcacgat attccccaga
     1981 ccttttcagt gtacgcttta aaaaaaattc ggaacaatcg ccgtccgaag cgattagtca
     2041 cccattgctc aaaagtctct tcctcaggat acggtcgcag ctgccatcta atataactaa
     2101 gggtgatcaa aatgctttgc cacaggccga gtccggccag agcattcatc ggtttcaatg
     2161 gataatcgaa gaactttcga ttgtagtaga ttctcgacag ccgcggccgg cgaagaaagt
     2221 tatcacccaa gacctctcgc cacgtcttgt ttacttcttc aactttagtg aagaaacgat
     2281 gtccgcccat gtcgaagtaa aaacccttat aaacttcggt gcgggcaatg ccgccgactt
     2341 tgtcgccttt ctcaatgacg attggcttgc cgtcgagttt ggccaactca tgggctgccg
     2401 tcaaacccgc gggaccggcg ccaattacta ccacctgctt tactccgggt cgtgggtcat
     2461 tcataaacaa acggtcgccc gccagattgt gctcaagttc ttttgaccgt gattgtctga
     2521 ctcgccgatt ttaatttgga tgccggtcgt gctttcgtta gcttcttgat tctaaattcg
     2581 gcacagacat agaaatagct cagcccgcta tatagattgt agaaaagatg aagcggaata
     2641 catgagatcg cgaatgacat cccccgctcg cgccggaaga acgcgtagag cccgcgattc
     2701 agaaagacaa ccccgctgag cgcaagcgct gacagggcga acgatccttg ccaaaccaga
     2761 cccagggcga cgaatgcgca agccgatccc atcagagcga cgctggcttt ttgcgccggc
     2821 gctagattta actcctgggg agcctttgta gtttcgtgaa tcagccgtga ccacgggacg
     2881 gcgcggcaga tgacgtcggt ccggattagg gagagaaaac tccatcgctt aagatgtgtt
     2941 ccttgaagtt gtttgtccaa tagaattcga tagccggcgc tcctcaggcg atatcccaac
     3001 tcgatgtctt caatcgaggg ccgcgaaaaa cgcttttcat caaaaccgcc gattcgcgtg
     3061 aaaacgctgc ggcgaattgc gccgcagcca gcccagaacg ttgaagcctc cgtattgccg
     3121 ttctgatgga cgaagtggtg cagcagattt ctatattgcg agatcattcc cgatgccgcg
     3181 gggtgagcgt cgtatgatcc aaaaaccgcc gccaaatggg cgtgctcctg aaatacggtc
     3241 agtacacggt caaacgctcc ggcggccaag acgacgtccg catcaacaaa aaagataacc
     3301 gcgccgaccg ccgcttccgc tccacggttg cgggcatagg cggggccaaa cgggccatca
     3361 gaaagatgga gcacacgcgc tccgaactgc cgcgcgatag acgccgaatc gtccgtcgaa
     3421 ccatcgtcca cgacgataca ttcccggata cggtcattcg caaacaccgc ctccaggcag
     3481 cggtttaaat ggagagcggc attgaatgcg ggaacgacga cggaaatcga cggtacctgg
     3541 atttcttcag tcgacatata cggccacagt gtttctgagt caacgctgga ggaagggata
     3601 aaaattcact ctcatctcta gtcagatgag tgagcattcc tagaagcgtg ggaacaggag
     3661 attaggaacc cgagcgtggc tccgccaggt ctgttctagg ggttgagtca ctggagagta
     3721 acgtttgacc cagaggtaga gcgccgacat ggcggtgatg aagaaacgct agcggtgatg
     3781 ggcaatttcg atgccgagat agttgcgctt accacccgta tttaattgta aaagaactac
     3841 ggggtatcga ggttttccag gctattatgt ctgatccatt ttggcctggc ggcagtcggg
     3901 cgcatgggcc gcatcggcca agtaaatagt tcttccgacc agcttcggca agcaccaacg
     3961 cgtccgcact aactgattcc gccaagttgc ttcggatagc gatcttggat gcgctgccga
     4021 ccatcccggc ccggcgacct gacgatctgt tttccggaag atttcgatcg gggacacgcg
     4081 actcctgctc agagcagatc ggggcccctg cctggggggg gggaatccgc tccgatttgt
     4141 gttcccgcaa cctattgaat ctgcgttcga aggaacggaa ttagtgctta tcgcgcctat
     4201 tgttgaaaat gccacattcg agcaaggagc agaggacctg ctgcttgaga ggattgtaga
     4261 aggtcaatta aatgtttgct cagcggtgct tacgtcaaag ttcggactcc gggcgggctc
     4321 cggataaact ggaaggatca actcttccgc tgagcgttca ggatctttaa atctctccac
     4381 gaggtatttc tcaagaacat ccgcaatgac gtcgttacct ttgccgttga ggtgcaagta
     4441 gtctctaaac agtgtctcac ctcccttcct gtagagggct tccgtcagat ccagcatagg
     4501 aataccaaac tcctcgcaaa tctcgcgaat cctccgctga ggataaagga taaacgcctg
     4561 atcgagcttc cggtactcat cgttaacctg gtcacttatg ggaaatacca gaaccatcag
     4621 cgggactccc ttttgtctca acaacgagcg catctcccga ataagagctc gagcgtggtc
     4681 ccacccatac ggctgccagg ccagatagaa atctcctcgc tgttcaaacg gaaacaccgg
     4741 gcggcccagt atcctcttcc acaggttttc agtcatgctt gcaagttgat gcgcaagatg
     4801 gcttcgagca aagagaattc caggaaactt ttctggatca aagtgatata ggtgtgcggt
     4861 cggttcgcgt ccgagaaatc tttgactatt cggcttgtga agatatttgt agtagacatc
     4921 gttgaacacg aacccgagca tgactaggtc cggcttcata tcaagaccgt agatcttgag
     4981 gaattcgagt tcctgatacg tggtatatcc tgggacgccg gcattgatga cttcgacagc
     5041 gcctggactg ttggtcaaac gagcattcaa ccgctgttcg aggacttcag tataaagttt
     5101 accggaacta gtctcaccag accagatcag cgaatcgccg agaaacagga ttcgtgaggt
     5161 tccggctttc ttcggtccca cttctcggtt tcgaagtcct aaactattcc ggccgtccga
     5221 cggcgaaaat ctccatcgaa gtggcatatc acgggcggtc agatagtcgg gcacatagag
     5281 cgcaggctca aacggccagg ctggaatcag gtatcgaacc agcaattcgc cggttagcag
     5341 cataaacaga attaacaacc ccacgacagt tagcttctgc atctttcaac aatctctaat
     5401 cgtctaattg cggcggctta gcggtacact ctgacttccg gtctatccta tctattgatc
     5461 gatattgctc gatgcacgat agactcgtta ggtccgaaga tcgcttaaag gactggctag
     5521 ttggtcttag atgctcgccg gttgcacctg ccgccgaaaa cccgttccaa ccacgaaaga
     5581 taggcctacg gccgtgacgg aaacaaattg acaaatatga gcgagcagcg catacgcgaa
     5641 agccatttca tgggtgaccc cgaacagtgc cagcgccagc acggtaaagt actcaaagtt
     5701 tccgacatac cctggtgctg ccggaagaat gacgccgagt ccgatcacca tctggataat
     5761 gagggccgtc caaaaagaaa gctcgagatc aagagcatgg aagagaagaa aaaaatacag
     5821 gccgtggaaa aaccagcaga cgaaagacag caaacaaaca ggcaacagcg cgccggcgta
     5881 attgatgcct ttcatgccat ggaggaattc cgtcgcaatc ggtgtcacgt gccgcgcgaa
     5941 acccatcggc attagcacca ttgccttatg tatccagagt tccgtccagc catgccggat
     6001 cgcgataaca agtcctacaa gcccggttat ataaagcgtc aaaagcgcga tgttgacacg
     6061 agtcacccat gggggaagct ctacggcggc aagcactatg aatggagtca gagaaatcac
     6121 cacgccatca aaaactcgct ctaaaacaat cgagccgagt gcagcagact tgctgatgtc
     6181 ttgcgtttga ccgatcgaca ccgcccgcat gatctccccc gcccgaaatg gaagcaaata
     6241 gtttcccata aaaccgatca cagtcgagcg gaaaagcgca cccacagcta tatccttaat
     6301 tggagc
//
LOCUS       DASUVS010000310         5560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_126921, whole genome shotgun sequence.
ACCESSION   DASUVS010000310 DASUVS010000000
VERSION     DASUVS010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126921"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VF182_16735"
     CDS             complement(<1..479)
                     /locus_tag="VF182_16735"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX6438786.1"
                     /translation="MPKAENSKAGRAATVQFVEPMYAQLVQQLPEGNEWLYEVKFDGY
                     RCLAGKDGAGVTLWSRRGNDLTAQFPAIATSCEQLPPRTLLDGEIVAIDTNGRISFNL
                     LQHHRSQAQALLFYAFDVLIHRGINLVNEPLSKRREVLSDLMKPFTRKATAVSLSES"
     gene            complement(588..1805)
                     /locus_tag="VF182_16740"
     CDS             complement(588..1805)
                     /locus_tag="VF182_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438787.1"
                     /translation="MPKLGNIPLVSDGTGEASGAAFTLVSPAKGNALLALDNAVIEVR
                     RGQPNVVVRFTSTGDPATAFAHGHQFAQQGLDLMSVLGRQDALIQDAEDEHALWWTEA
                     DGLVVRLVSTTILELAVGSMTAVVHDKEGNVIPQATPQPRHHIGFRYYRLAQVTDDLF
                     DAFRNMYLAFEALLSTQYPIAKGEREIDWLRRALSAGSATLPLGSLRISPEADPVEYI
                     LDAVYKDTRLPLFHAKEGREYFPPQDSPTDREVVSRALGVLTHIVIGIANSWYDARRQ
                     GGGFFFSWIYNNVKSMLTNASVCFSNHDGPYDPNESDLSHPRFEKAVKAACKLSAQLG
                     RGREPAIFASVRGTDVSSLNRLRRIEVVTPQHPVIAHILEAELECSGTMRLEALIHVR
                     AMNLNQPKSLFRR"
     gene            complement(2108..2803)
                     /locus_tag="VF182_16745"
     CDS             complement(2108..2803)
                     /locus_tag="VF182_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX6438788.1"
                     /translation="MMQAETRAILEHLLVALTAGALIGLERSYHGRPAGFRTHTLVCI
                     ASSTLMLVTMYPGRWFPDPVSGSVSVDPTRMAQGIMTGIGFLGAGVIFKEGLTVRGLT
                     TAASIWITAAIGILAGIGFYFPVIVATVLTLGTLSVFRWIEAKMPVQFYARLEVKLPR
                     DASDSEEELQRLIGEHGFTIANMNYHHDKNRGFSEYGMVIRTDNAANVQRLVNTLHNM
                     VTVQEFKMSPMGD"
     gene            complement(2886..4814)
                     /locus_tag="VF182_16750"
     CDS             complement(2886..4814)
                     /locus_tag="VF182_16750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX6438789.1"
                     /translation="MGVAQVAPYGSWKSPITAEVVARGEIGLEQVRIDGEDVYWIERR
                     SQEGGRKVVVRRSPDGTIVDVTPLRFNARTRVHEYGGGDFAVSAGTVAFANFADQRLY
                     IQRAGGEPKSVTPAGELRYADLVVHPGRDQLFCVREDHTGPGQAINALVSIELDQENP
                     GRVLVSGNDFYSSPRLSPDGSRIAWLTWNHPAMPWDGTELWVAEFSPDGSLNQPRQVA
                     GGQAESIFQPEWSPGGTLYFTSDRTGWWNLYRCNENRVEAVCPMEAEFGQPQWTFAGS
                     LYAFDSESKIICSYSKTGRSYLAEIDATTGALAPFELAYSAISRVQTAAGRAVLIAAS
                     ATEPTSIVQLNLAAGDVTLLRRSREVELDPGYFSTPRPLEFPTESGQTAHAIFYPPYN
                     RDYVGDENEKPPLLVMSHGGPTASTSMSLHYSVQYWTSRGIAVLDVNYRGSSGYGRAY
                     RELLNGRWGVVDVDDCVNGARYLAETGQVDGERLAIRGGSAGGYTTLCALTFRDTFKA
                     GASHYGISDLEALAKDTHKFESRYLDGLIGPYPERRDLYVQRSPIHFTERLACPMILF
                     QGLEDKVVPPNQAEMMVEAVRAKGLPVAYLTFDGEQHGFRRAENIKRVLEAELYFYSK
                     IFGFDLADAIEPIEIHNL"
     gene            complement(4887..>5560)
                     /locus_tag="VF182_16755"
     CDS             complement(4887..>5560)
                     /locus_tag="VF182_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HEX6438790.1"
                     /translation="VTNKQLPLRIAMAYGIYRTRDDGPDSSTAAQFKTRFSREIKIKF
                     LGVWDTVGALGIPLDLLKRLNMHFYEFHDTRLSTIVENAYHAIAVDEHRIDYDACLWN
                     PEVKPEQVLQQRWFLGAHCDVGGGYRDRRLSDVALRWMQDNAAVAGLALDLVMVGPEN
                     YRGVFTDSYGEFLGGVYAKKNPRHYRAVGATRFGNESVDESVGKRRKEDRDYEPQNDG
                     LPPLA"
BASE COUNT         1264 a         1548 c         1498 g         1250 t
ORIGIN      
        1 atactctcag ataacgaaac tgcggttgcc tttcgggtga acggtttcat taagtcgctt
       61 aggacttcac gtcgtttgga gagtggctcg ttgactagat tgatcccacg atggatcagg
      121 acatcgaagg cataaaacaa caatgcttgt gcttgcgatc gatgatgttg aagcagattg
      181 aatgagatac ggccattcgt gtcgatggct acgatttcgc cgtcgagtag tgtgcgcggc
      241 ggaagctgtt cacatgatgt ggcaatcgca ggaaattggg ccgtcaagtc attgccgcgt
      301 ctagaccaca acgtcactcc tgcgccgtct tttccagcga ggcagcgata gccgtcaaat
      361 ttgacttcgt agagccattc gttgccttcg ggtagttgct ggacgagctg agcgtacatt
      421 ggttcgacga actgaaccgt tgccgctctg ccggctttag agttttctgc tttcggcatt
      481 tggatggagt tacgacgtga agagagccga ggcgatctcg cagccgcagg tagcacagcg
      541 cttgtgaacg ggtgttagcc cagcttccgt gaaggttcgt gaaatcatta tctgcgaaat
      601 aacgacttag gctggttcaa gttcattgct cgaacgtgta tcagagcttc aagccgcatc
      661 gtaccactgc attctagctc ggcctcaaga atgtgggcaa ttactggatg ctgtggtgtg
      721 acaacttcta tgcgccgtaa tcggttgagt gaagaaacat cggttcccct aacggacgcg
      781 aaaatagcag gctctcgccc gcgccctaac tgcgcggata gtttgcaagc ggcctttaca
      841 gccttctcga accggggatg ggacaagtcg gattcgttgg ggtcataagg gccgtcgtga
      901 ttgctgaaac agacagacgc gttcgttaac atagacttga cgttgttgta aatccaagaa
      961 aagaaaaacc cgccaccctg gcggcgagca tcgtaccaag aattggctat cccaatcact
     1021 atatgggtga gcacgccaag tgcgcgggag acaacttccc gatccgtagg cgaatcctgt
     1081 ggtggaaagt attccctccc ctctttggca tgaaataaag ggagccgggt gtctttgtaa
     1141 acggcatcca gaatatattc aaccgggtca gcttcggggc tgatcctcaa actacccaac
     1201 ggaagagtcg cgcttccagc actcaaagcg cgacgtagcc aatcgatctc gcgttcgcct
     1261 ttcgcgattg gatattgagt actgagcagg gcctcgaagg cgagatacat gttgcgaaaa
     1321 gcatcgaaga gatcgtcggt cacttgtgcc aggcgatagt agcgaaaccc gatgtgatgg
     1381 cgcggttggg gtgttgcttg gggtatcaca tttccttctt tatcgtgcac gacagcagtc
     1441 atcgatccga cggcgagttc aagtatcgtt gtcgacacaa gacgcactac aaggccatct
     1501 gcctcagtcc accagagtgc gtgttcgtct tctgcatcct gaatcagagc gtcctgtctt
     1561 ccaagcacgg acatcaggtc gaggccttgc tgggcgaact ggtgcccatg cgcaaatgca
     1621 gttgcgggat ctcccgtact ggtaaaccgc acgacaacgt tgggctgtcc tctccgaact
     1681 tcaatcaccg cattgtcgag ggccagcaga gcgttacctt tagctgggga aacaagagtg
     1741 aacgctgcac cggatgcttc accggtgccg tcgctgacca agggaatgtt acctaatttt
     1801 ggcataacga aattatcctg gctttaggcg cttagtgtgc gaagcgccac acgataccga
     1861 tcacacgaga cgctgactcg tgaagggatg acggttgttg gcggagttcc aagaatggcg
     1921 gggtgattgc tgagcggtca tggcgttctc tcattgtcgg cacattccag tgtctggtca
     1981 accgatctaa atcgtgcttg acacgcgtgt gtttcagacg gacagccatt ccacgtatta
     2041 aaacttttgc atatcaagcg atatttctga acgaacgatc ttgccgatgt gccaaaaaac
     2101 gatgccttca gtcgcccatg ggcgacattt tgaactcttg aaccgtgacc atgttatgca
     2161 gcgtattcac caagcgctgc acattggcag cgttgtcggt tcgaatcacc atgccgtatt
     2221 cggagaatcc ccggttttta tcatgatggt agttcatatt ggctatagta aagccatgct
     2281 cgccgattaa ccgctgcagt tcttcttcag agtccgacgc atcgcgaggg agtttcactt
     2341 ctaatcgagc gtagaactgg acgggcatct tggcttcgat ccatctgaat accgaaagag
     2401 tacccagcgt aagcacagtg gcgacgataa ccggaaagta gaagccaatg ccggcgagga
     2461 taccgatggc tgccgtgatc cagattgaag ccgcggtcgt caggccgcgg acagtcaacc
     2521 cctctttgaa aatcactccg gcgccgagaa agccaatgcc ggtcatgatg ccttgcgcca
     2581 tgcgtgtcgg atctacacta acgcttcccg atacaggatc aggaaaccat cgtcccgggt
     2641 acatcgtcac taacatcaaa gtgctggaag cgatgcagac taaagtgtga gtccggaaac
     2701 ccgccgggcg gccgtgatag ctccgctcta agccgatcag cgcgcccgct gtaagagcca
     2761 caagtaaatg ttcaagtatt gccctggttt ccgcttgcat cattcaaagc tcctcgacac
     2821 cagatgaaag tatctaagtt actccctgcg cggcgtgcca cgacgcctta agcttccgcg
     2881 ataaatcaaa ggttatgaat ttctattggc tcgatcgcat cggccaaatc gaacccgaat
     2941 attttcgaat aaaagtagag ttcggcctcc aagactcgct tgatattctc ggctctgcga
     3001 aacccatgct gctcgccgtc gaatgtaaga taggccaccg gcagtccctt ggcgcgcact
     3061 gcctcgacca tcatctccgc ctgattcggc ggcacaacct tatcttccaa gccttgaaac
     3121 aaaatcatcg gacaggccag gcgttcggtg aaatgaatcg gcgatcgttg gacgtaaaga
     3181 tcacgccgct ccgggtacgg accaatgagc ccgtcgagat agcgcgattc gaatttgtgc
     3241 gtgtccttcg ccaacgcctc caaatcgctg atcccataat ggcttgcccc ggctttgaac
     3301 gtgtcgcgaa aagtcagagc gcatagcgtc gtatagcctc cggcgctgcc ccctctaatc
     3361 gccagccgct cgccatcgac ctggccggtt tctgctaaat atctagcccc gttgacgcag
     3421 tcatcgacat cgacgactcc ccaccggccg ttgagaagtt ctcgataggc gcgcccgtaa
     3481 cccgagctgc cgcggtaatt gacatcgagc acggcaatcc ctcgactggt ccaatattga
     3541 actgaataat gcaacgacat cgaagtcgac gcggtcggtc cgccatggct catcaccagc
     3601 agcggcggct tttcattttc atcaccgacg taatcgcgat tgtatggcgg atagaaaatt
     3661 gcgtgagcag tctgaccgct ctcggtcgga aactccagtg gtcgcggcgt ggagaaatat
     3721 ccgggatcta gctcgacctc tcgggagcgg cgtagcagcg tcacgtcgcc ggcagcgaga
     3781 ttcaactgga caatggaggt tggctcggta gcagatgcgg cgattaacac ggctcgacca
     3841 gccgctgttt gaacccgcga gattgccgaa taggctagct cgaacggcgc caacgcgccg
     3901 gtggtcgcgt cgatctcggc taagtacgag cggccggttt tcgagtagct gcagataatc
     3961 tttgattcgg agtcgaaagc gtacaacgaa ccagcgaatg tccactgcgg ctggccgaac
     4021 tccgcttcca tcggacacac tgcttccact cgattctcgt tgcagcgata taaattccac
     4081 cagccggttc gatcggaagt aaagtaaaga gtgccgcccg gcgaccactc cggttggaaa
     4141 atggattcgg cttgtccgcc ggccacttgt cttggctgat tcagcgaacc atcggggctg
     4201 aactccgcga cccaaagttc ggtaccgtcc catggcatgg cgggatgatt ccacgtcagc
     4261 caagcgatgc gcgagccgtc cgggctcaaa cgtggcgaag agtaaaagtc atttccggac
     4321 accaaaactc ttcccggatt ctcctgatcg agctcgatgc tcaccaaagc attgatcgcc
     4381 tgaccaggac cggtatgatc ttcgcgcacg cagaataatt gatctctacc cggatggacc
     4441 acaaggtcgg catagcgcag ctctcctgcc ggggtcacag atttgggctc gccaccggct
     4501 ctttggatgt agagccgctg gtcggcaaag ttggcgaacg caacggttcc ggcagaaacc
     4561 gcgaagtctc cgcctccata ctcgtgcact cgcgtgcgag cgttgaagcg caaaggcgtg
     4621 acatcgacga tggttccgtc gggcgatcgc cgcacgacaa cttttctccc gccttcctga
     4681 gaccgccgct cgatccagta tacgtcctcg ccatcgatcc ttacctgctc gagcccgatc
     4741 tcaccgcgcg cgacaacctc ggcggtgatg ggtgacttcc acgagccata aggagctact
     4801 tgcgcgacgc ccataaactt ctcctgttat ccagaggaga tcccctcgtg gacgtttttg
     4861 ccatgtcaac ttcgagctct aatatctcac gccagcggcg gtagcccgtc gttctgcggc
     4921 tcatagtcgc gatcttcctt gcgccgcttg ccgaccgatt cgtctacact ctcattgccg
     4981 aaccgggttg cgccgacggc acgatagtga cgtgggtttt tcttggcgta cacgcctccg
     5041 agaaattccc cataagaatc ggtaaagacg ccgcggtagt tttctggccc gaccatgacc
     5101 aaatccagcg cgagtccggc aacggcggcg ttatcctgca tccagcgcag cgcaacatcg
     5161 gataaacggc gatctctata gccgccgccg acatcgcagt gagcgccgag aaaccatcgc
     5221 tgttgtaaaa cctgctcagg ctttacttcg ggattccaca ggcaggcatc gtaatcgatt
     5281 cggtgctcgt cgacggcaat ggcgtgatag gcgttttcga caatcgtgct gagccgggta
     5341 tcgtgaaact catagaagtg catattcaag cgtttcaaca aatccagcgg gatgccgagc
     5401 gcgcccacgg tatcccaaac tccaagaaac tttatcttga tctcgcgcga gaatctggtc
     5461 ttgaattgcg ctgcggtgga ggaatccggg ccatcatctc gggtgcggta aatgccatag
     5521 gccatggcga ttcgcaacgg caactgcttg ttcgtcacca
//
LOCUS       DASUVS010000311        10016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_565865, whole genome shotgun sequence.
ACCESSION   DASUVS010000311 DASUVS010000000
VERSION     DASUVS010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10016
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565865"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2443
                     /gene="fdnG"
                     /locus_tag="VF182_16760"
     CDS             <1..2443
                     /gene="fdnG"
                     /locus_tag="VF182_16760"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HEX6438791.1"
                     /translation="LAASFGRGAMTNHWVDMLNADVVLIMGGNPAEDHPIAMNWLARA
                     KEQGTYILHVDPRFNRTSSIADVFAKLRSGTDIAFVGGMINYAIEHNRIQQEYVREYT
                     NASWIVGDGYSFEDGLFAGYDPKSRRYDRSLWAYARDAQGRPRRDPTLKDPRCVFQLL
                     KQHFSRYDPDAVCRITGTPKENYLKICDLYTSTSAPDRAGTWLYAMGTAQHSHGTQNI
                     RSYAILQLLLGNIGVAGGGVNAMRGESNVQGSTDQGLNWDGLPGYLRLPVEADADLKT
                     YLSRVTPTSLDPANSANWLQNTPKYVVSLLKAWWGERATSENDFALNYLPKLGAGFQG
                     QGYAFLALTHAMLAGEIKGLFCFGQNPAVGGANARLIRAGLDKLEWMVVADLFEHETA
                     GFWKRPGVDPAKIPTEVFVLPACSGVEKEGSIVNSGRWAQWRHQAIKPIADSRPDLDI
                     VDGLAKAMKRIYEKDGVFPEPILHLAWDYGEHADPHRVARELNGRFLVNVTEKDGKEF
                     AAGKQVPGFAQLRDDGSTMSGNWIYCGGYTEEGNLAARRDTSDAPNKIGLHPKWGWTW
                     PMNRRILYNRASVNRKGEPFNPRKWVIRWNAEQKAWEGDVPDGAMPPGEMNPFIMLAS
                     GVGQLYSPDVGDGPFPEHYEPVESPVKNPFSRIQSSPCAQVWQSTEFDKYGKPDVFPI
                     VATTYRVSEHWQTGAMSRNMPWLAGLMPSAFVEIGTALAQREEIKNGDRVIVSSARGA
                     IEVYALVTERFQPFFIEGRMIDEIGLPWHWGYAGIVPGDICNDLTASVGDANSHIPET
                     KVFLCNIKRKDRASV"
     gene            2834..3613
                     /locus_tag="VF182_16765"
     CDS             2834..3613
                     /locus_tag="VF182_16765"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6438792.1"
                     /translation="MIQDNTRCIGCRACMVACKSWNDRPEDKTDFFAGEGYQNPRDLD
                     ANNYNLITFNEIVTGAKPDWVFGRQLCMHCEHPACASACPTTALNKTPIGAVVFDESR
                     CIGCRACMQACPFIIPKYDYDSFAPKIHKCTFCADRLEVGMDPACATVCPTGAITFGD
                     RDDMVAEAKRRIAAAPGTYIPEIYGLEEVGGTSVLHLSSVPFEQIGYITNLPKTPMPD
                     LTHRWLRVTAPVFAVLYAVFGALNWIVRRRMRNQAHEGEHT"
     gene            3610..4791
                     /gene="nrfD"
                     /locus_tag="VF182_16770"
     CDS             3610..4791
                     /gene="nrfD"
                     /locus_tag="VF182_16770"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX6438793.1"
                     /translation="MNWRIRLTWWDKLLLAIMAVAAIIAFVRFATGIGTIANINNAYP
                     WGWWVGYGIMTMIAIGGVGFTMTALVEIFGVHRYHSFLRPAVLMGLLCYISAITMLMV
                     ELGRPWKVWMVLVSWAPTSALYEVGWCAFLYLTVLALEFAQVPVERLGWGRALRVLRL
                     IYIPVMLLGVTLSHLHQSSLGTLMTLIPHKINVLWWSDNLPLLYLFSAMMAGPAMAIL
                     EHLAAARWLGFAPRMNLLAGLARIEAWIVGLFLAFQLGDLFYRGAVDAMFIASWFAVS
                     FWVEIGLGLLLPFVLLLMPEVRESRRGLATACSLVIAGILLHRLNVAVIGLRVRHWET
                     YVPSLGEVGITLGITAAAVFAFGYLARILPIHEELPAPETRPLPAARAAGAWHGVEGV
                     S"
     gene            4769..5380
                     /locus_tag="VF182_16775"
     CDS             4769..5380
                     /locus_tag="VF182_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438794.1"
                     /translation="MAWKEYRDMAAEQPPRFPAFRVIVLLALVVNLILTVAVVTQVRE
                     VQQRVASLPPDLAIKRDVAMLRPLRIREIITQNCVECHSSRRLGATVSMEPSDVQRTV
                     ERMQTHPGANIPPGEFERITASLLVARCARCHGEETLNLMVLKTQPERIATIRRMAAL
                     PGSGVRADQVLAIEQAFEKLISEGDRRRVDPATGNYAVPINKQ"
     gene            5401..6183
                     /locus_tag="VF182_16780"
     CDS             5401..6183
                     /locus_tag="VF182_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase cytochrome b subunit"
                     /protein_id="HEX6438795.1"
                     /translation="MSNQVDVITGVGLDQRTRKSHWPARLDFIQSASGLALGLFMWGH
                     MAFVSTILISNDAMWRVTKMFEGEFILGKAYAGIVAAVVAVVTFLFVLHGLLAMRKFP
                     ANWNEYKSFAGHRQMLRHEDTTLWWVQAWTGFALFFLASVHLYQMLMNPGDIGPYESA
                     ERVWSGGWWPLYLVLLFAVELHGGVGLYRLAVKWGWFEGKDPNRTRIILKRLKWGITI
                     FFLTLGLLTLAAYMKLGHERRDRLGERYVPTYMAPSTSPGHR"
     gene            6416..8431
                     /locus_tag="VF182_16785"
     CDS             6416..8431
                     /locus_tag="VF182_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase flavoprotein subunit"
                     /protein_id="HEX6438796.1"
                     /translation="MKIIYTDVLVIGGGLAGLRIGIGAKRRGHDVIILSLVPPKRSHS
                     AAAQGGMQASLANVIKGQGDNEDVHFEDTVRGSDWGADQQVVRMFVDTAPKAVRELAA
                     WGLPWSRVTRGDRDVIINGQKVTITERDEAHGLIAQRAFGGTNKWRTCYVSDGTGHAM
                     LNTMSDQAIAQKIPVHERAEAIALIHDGSRCYGAVVRNLVTGELAAYVAKATAITGGG
                     AGRLYRVTTNAVISEGIPTAIALETGVATLGNMEAVQFHPTPAFPPGILLTEGARGDG
                     GLLKDVDGHRFMPDYEPEKRELASRDVVSRRMEEHIAKGKGVKTPFGDHLWLDITLLG
                     EHHIKHNLREVYEICHYFLGVDPVKEWVPVRPAQHYTMGGVRTNHTGESPTLKGLFSA
                     GEAACWDMHGFNRLGGNSVAETVAAGMIVGEFIADFCERSENAANFPAGMVQETLRRE
                     QAKIDVMIDGRGTENAAALMAEMQTIMTNKVGIFRSGRVLEEAVHELQQLLLRSRNIG
                     LRYKARGANPELVTAYRVQKMLKMAQCVAYGALQRTESRGAHYREDFPRRNDAEWLNR
                     TLATWKSENDTLPSLAYEQLDVMKMELPPGWRGYGAKDHIDHPDTSRRLAEIEAVNQG
                     RDGADRFSVQQKLMPYEHLLPKPLRGRNERIDERRTERTSPAGLRAG"
     gene            8507..9904
                     /gene="fumC"
                     /locus_tag="VF182_16790"
     CDS             8507..9904
                     /gene="fumC"
                     /locus_tag="VF182_16790"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004778917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX6438797.1"
                     /translation="MAETRNERDTMGEIAVPSDRYWGAQTQRSLKYFQAGAGRDTMPR
                     ELIKACGILKKAAAMANVELKLLDQERGDLISQVADEVIAGKLDEHFPLVIWQTGSGT
                     QTNMNVNEVISNRAIEIAGGVMGSKKPIHPNDDVNKAQSSNDTFPTAMHIAAVEVLAS
                     LVEALKDLKKHLDDKASAYANVIKIGRTHLMDATPLSLGQEISGWAQMLENNLQRIDA
                     TLPHLYQLALGGTAVGTGLNTHPEFAEKAAAKIAALTNRPFVSAPNKFEALAGHDALV
                     FAHGALKTLACSLMKIANDVRWLASGPRAGLGEISIPENEPGSSIMPGKVNPTQSEAL
                     TMICAQVLGNDVAINVGGASGNFELNVYKPLMIHNFIQSAKLLSKGVAGFTQHCIAGL
                     EPNADRIDELMKKSLMLVTALNPHIGYDNAAKIAKKAHAEGTTLKEAALALGLVKAED
                     FDKWIKPEEMITPGM"
BASE COUNT         2092 a         2923 c         3043 g         1958 t
ORIGIN      
        1 tctggcggcc tcattcggca gaggggcgat gaccaaccat tgggtggata tgctgaacgc
       61 cgatgtcgtt ctgatcatgg gcggaaatcc ggcggaggat caccccattg cgatgaactg
      121 gctggcgcgc gccaaggagc agggcaccta tatcctgcac gtcgacccgc gcttcaatcg
      181 cactagctcg atcgccgatg tattcgccaa actgcgctcc ggcaccgaca tcgctttcgt
      241 cgggggcatg atcaactacg ccatcgaaca taatcggatt cagcaggagt atgtacgcga
      301 gtacaccaat gcctcctgga tcgtcggcga cggctacagc ttcgaagacg gtcttttcgc
      361 gggttatgat ccgaagagcc gtcgctatga ccgctcgctt tgggcttatg caagggacgc
      421 gcagggccgg ccacggcgcg atcccaccct caaggatcct cgctgtgtgt ttcaactctt
      481 gaagcagcac ttctcgcgct acgatcccga tgcggtgtgc cgcatcacgg ggacacccaa
      541 agaaaactac cttaagattt gcgatctcta tacttcgacc agcgctccgg accgggccgg
      601 cacgtggctt tacgccatgg gcaccgcgca gcacagccat ggcacccaga acattcgatc
      661 atacgcgatc ctgcagctct tgctcggcaa catcggggtt gccggcggcg gcgtcaatgc
      721 gatgcgcggc gaatccaatg tccagggttc cacggatcag ggactcaact gggacggcct
      781 gccgggctac ctgcggcttc ccgtcgaagc tgatgcagat ttgaaaacct atctctcgcg
      841 cgtaacgccc accagcctcg atcctgccaa cagcgcaaac tggctgcaga acacaccaaa
      901 atatgtcgtg agcttgctga aggcgtggtg gggagaacgg gctaccagcg agaatgattt
      961 tgctcttaat tatctgccga aactgggcgc cggatttcaa ggacagggct acgcgttcct
     1021 tgcgcttacc cacgccatgc tcgccggcga gatcaaagga ctcttctgtt tcggacagaa
     1081 ccccgccgtg ggcggggcca atgcgcgcct gatacgcgcg ggcttggata agctcgagtg
     1141 gatggtggtc gccgatttgt tcgagcatga gaccgcgggc ttctggaaac ggccgggcgt
     1201 cgatccggcg aagattccca cggaagtctt cgttctgcct gcctgttccg gcgtcgagaa
     1261 ggaaggcagc atcgtcaaca gcgggcgttg ggcgcagtgg cggcaccagg caattaagcc
     1321 catcgcggac agccgccccg atctcgatat cgtcgatggc ctcgccaaag cgatgaagcg
     1381 catctacgag aaggacgggg tgtttcccga gccgatccta catctcgcct gggactacgg
     1441 agagcatgcg gacccgcacc gggtcgcgcg cgagttgaac ggacgcttcc tggttaatgt
     1501 aaccgaaaaa gacggcaagg agttcgctgc cggcaaacag gtgccgggct ttgcgcagct
     1561 acgcgacgac ggcagcacca tgtcgggcaa ctggatttac tgcggcggct acacggaaga
     1621 aggcaacctg gcggcgcgcc gcgatacgtc cgatgcgccg aacaagatcg gactccaccc
     1681 gaagtgggga tggacgtggc cgatgaaccg gaggattctc tacaaccgtg cgtcggtcaa
     1741 tcgcaaaggc gagccgttca acccccggaa atgggtgatc cgctggaacg ccgagcagaa
     1801 agcatgggaa ggcgacgtcc ccgacggcgc catgccgccg ggcgagatga atccctttat
     1861 catgctggca tcgggggttg ggcaactcta ctctccggat gtcggggacg gtccattccc
     1921 cgagcactac gaaccggtgg agagcccggt gaaaaatccg ttctccagga tccagtcgag
     1981 tccttgcgcc caggtttggc agtccaccga gtttgataaa tacggcaagc cggatgtctt
     2041 tccgatcgtc gcaacgacgt accgagtttc cgagcattgg cagaccggcg ccatgagccg
     2101 caacatgccc tggctcgcgg gtctgatgcc cagcgcattc gttgagatcg gtacggcgct
     2161 ggcccagcgc gaagagatca agaacggcga ccgggtcatc gtcagcagcg cgcgcggcgc
     2221 catcgaggtg tacgcgctcg tgacggaacg cttccaaccg tttttcatcg agggccgaat
     2281 gatcgacgag atcggcctgc cgtggcactg gggctatgcg gggatcgtgc ccggcgacat
     2341 ctgcaatgat cttaccgcca gcgtcggcga cgcgaactcg cacatccccg agaccaaagt
     2401 gtttctttgc aacatcaaaa gaaaggaccg cgcatcggtc tagcgcagcg cagtcggaaa
     2461 gaatcggaac ggatgactca ccacgattta atatgagctt gcgctcgtca ctgacaatga
     2521 agatggtgtt ttgccattta agtttttcta gttgtcattc ccgcgtaggc gggaatccag
     2581 gttgtattac tgttcgcatg agcctggata cccgctttcg ccggtatgac ggaattgcag
     2641 cagtggctct atcactctcg cgatcagcca caaggcgttt caaaggcgaa aaaaatcgcg
     2701 aaggaaaatc cagtcagtct cttttccgaa cttcgtgtcc tccgcgcgct tcgtggtgaa
     2761 ttcaggccga aaaatttgct gtaacacgaa aagggagccg taaatgagca caccgaccgt
     2821 taagccgaaa gcaatgattc aggacaatac ccggtgcatc ggctgccggg cgtgcatggt
     2881 ggcgtgtaag tcctggaacg accggccgga agacaagacg gatttcttcg ccggcgaagg
     2941 ctatcagaat ccgcgcgatc tcgacgccaa caattacaat ctgattactt tcaacgagat
     3001 cgttaccggc gccaagcccg attgggtgtt cgggcgccag ctctgcatgc attgcgaaca
     3061 tcccgcctgc gcatcggcgt gcccgaccac ggcgttgaac aaaacaccga tcggcgcggt
     3121 ggtcttcgat gagtcgcgct gcatcggctg ccgcgcgtgc atgcaggcat gcccattcat
     3181 cattccgaag tacgactacg acagtttcgc gccaaagatc cacaaatgca ccttctgcgc
     3241 cgaccgtctg gaagtcggaa tggacccggc ttgcgcgacc gtatgcccca ccggcgcgat
     3301 caccttcggc gaccgcgacg atatggtcgc cgaagcgaag cggcggatcg ccgcggctcc
     3361 cggcacctat atccccgaaa tttacgggct cgaagaagtg ggcggcacca gcgtgctgca
     3421 cctttcatcc gtgccgttcg agcagatcgg ctatatcacc aacctgccga aaacgccgat
     3481 gcccgatctc acccatcgct ggctgcgtgt caccgcgccg gtgtttgctg tcctatatgc
     3541 agtgttcggc gcgctcaact ggatcgtgcg ccggcgcatg cgcaaccaag ctcatgaggg
     3601 agagcacaca tgaactggcg gatccgtctt acctggtggg ataaattgtt actcgcgatc
     3661 atggcggtgg cggccatcat tgccttcgtg cgattcgcca ccggtatcgg caccatcgcc
     3721 aatatcaaca acgcctatcc gtggggctgg tgggtcggct acggaatcat gacgatgatc
     3781 gccatcggcg gcgtcggctt cacgatgacg gcgctggtcg agatcttcgg cgtgcatcgc
     3841 taccattcgt ttttgcgccc cgccgtgctc atggggcttc tctgctatat cagcgcgatt
     3901 accatgctca tggtcgagct gggccggccc tggaaggtct ggatggtttt ggtctcgtgg
     3961 gcacccacct ccgcactata cgaagtcggc tggtgcgcgt ttctttatct cacggtactc
     4021 gccctggagt ttgcccaggt gccggtcgaa cggctcggct gggggcgcgc gctgcgtgtt
     4081 ctgcggctga tttatattcc ggtcatgctg ctcggcgtga cgctgtcgca cctacatcag
     4141 tcgtctctcg gtactctgat gacgctcatt ccgcacaaga tcaatgttct gtggtggtcg
     4201 gataatttgc cgttgcttta tctgttttct gcgatgatgg ccggcccggc gatggcgatt
     4261 ctcgagcacc ttgccgccgc ccgctggctc ggcttcgccc cgcgcatgaa tctactcgcc
     4321 ggcctggcac gcatcgaagc ttggattgtc ggactctttc ttgcttttca gctgggcgat
     4381 cttttttatc gcggcgccgt tgacgccatg ttcatcgctt catggttcgc cgtctccttc
     4441 tgggtcgaga tcggtcttgg cctgttgctt ccatttgtgc tgctgctgat gcccgaggtg
     4501 cgcgagagcc ggcgcgggct tgccacggca tgcagcctgg tcattgccgg catattgctc
     4561 catcggctca acgtagcagt catcggtttg cgcgtgcggc actgggagac ctacgtgccg
     4621 tcattgggcg aagtcggcat cacgctcggt attacggcag cggccgtatt cgccttcggc
     4681 tatctcgcgc gcatcctgcc catccacgaa gaattgcctg cgcccgagac aaggccactt
     4741 cctgcggctc gagctgccgg agcgtggcat ggcgtggaag gagtatcgtg atatggccgc
     4801 agaacaacct ccgcgatttc ccgcttttcg cgtcatcgtc ctgctcgcgc tggtggtaaa
     4861 ccttatcctc acggtcgccg tggtgacgca ggtgcgcgag gtgcaacagc gagtcgccag
     4921 cctgcctccc gatctggcga tcaaacgtga tgtggccatg ctgcggccac tgcgtattcg
     4981 cgagatcatc acgcagaact gcgtggagtg tcacagttcc cggcgtctcg gggcaaccgt
     5041 atcgatggag ccgtcggatg ttcagcgaac ggtcgagcgc atgcaaaccc acccgggcgc
     5101 caatattccg ccaggcgagt tcgagcgcat caccgcttcc ctgctggtgg ctcgctgcgc
     5161 gcgctgccac ggcgaggaga cgctcaacct gatggtcctc aagacccaac ctgagcgcat
     5221 tgccacgatc cggcgtatgg cggcgctccc gggctcgggt gtgcgcgcgg atcaggtgct
     5281 cgccatcgag caagcgtttg agaaactgat cagtgagggc gaccgccggc gggtcgatcc
     5341 agcgacgggg aattacgctg tcccgataaa caaacagtag agttaagaag gagcggtgcc
     5401 atgagtaatc aagtcgatgt cattaccgga gttggactgg atcaacgcac acgaaagagc
     5461 cactggcccg cgcgcttgga ttttatccag agcgccagcg gattggctct gggtcttttc
     5521 atgtggggcc acatggcgtt cgtatcgacc atcctgatca gcaacgatgc catgtggcgg
     5581 gtgaccaaga tgttcgaggg agagtttatc ctcggcaagg cctatgccgg tatcgtcgcg
     5641 gcagtcgtcg ccgtggttac gttcttgttc gtgctccatg gcttgcttgc gatgcgaaaa
     5701 tttccggcga actggaatga gtacaaaagt ttcgccggac accggcaaat gctgcgccat
     5761 gaagacacca ccctgtggtg ggtgcaggcg tggaccggtt tcgcgctgtt ctttctggct
     5821 tccgtgcatc tataccaaat gctcatgaat cccggcgata tcggccccta cgaatcggcg
     5881 gagcgcgtct ggagcggcgg ctggtggccg ctctatctgg tcctgttgtt tgccgtcgaa
     5941 ctgcatggtg gcgtcggtct ctatcgcttg gcggtaaagt ggggctggtt cgaaggcaaa
     6001 gatccgaatc ggacgcgtat catactgaaa cggctgaagt ggggaatcac gatatttttt
     6061 ctgacgctcg ggctgctcac attggcggcg tacatgaagc tcggccacga gcggcgcgat
     6121 cgactcggcg agcgctacgt tccgacatac atggcgcctt cgacatcgcc aggacaccgc
     6181 tagagaaaac agagtctaag ggaaaaaatc gaagcacgaa gcccaagagt gaattcgaag
     6241 cacgaaatcc gaaacaattt caaatgaccg gaataggcaa attcaaaaca aacactcgag
     6301 atggaatcgg ggttttgaat ttttcgagtt tggacgtttt gtgccgattt gtttcggatt
     6361 tcgatactcg gattgcggat ttgtactcgg agattcagac gaaggaagct aaaccatgaa
     6421 gataatttac acggacgttc tcgtcatcgg cggcggcctg gcgggtttgc gcatcggcat
     6481 cggcgcgaaa cggcgcggcc acgatgtgat cattctatct ctggtgccgc ccaagcgttc
     6541 gcattcagcc gcggcgcagg gcggcatgca ggcgagcctc gccaatgtca tcaaaggcca
     6601 gggcgataac gaggacgtgc actttgaaga cacagtgcgc ggcagcgact ggggcgccga
     6661 ccagcaagtc gtgcgcatgt ttgtcgatac cgcgcccaaa gcggtgcgcg agctggcggc
     6721 ctggggcctg ccctggagtc gggtaaccag aggcgatcgc gatgtcatca ttaacgggca
     6781 aaaggtcact attaccgagc gcgacgaagc ccatggactg atcgctcagc gggccttcgg
     6841 cggcaccaac aaatggcgca cctgttacgt ctcggatggc accggtcacg ccatgctcaa
     6901 caccatgagc gatcaggcca tcgcgcagaa gatacccgtg catgaacggg cggaagcgat
     6961 cgctttgatt cacgacggca gtcgctgtta cggcgccgtg gtgcgcaatc tcgttaccgg
     7021 cgagttagca gcttatgtgg ccaaggccac cgctatcacc ggcggcggcg cggggcggct
     7081 ctaccgcgtg accaccaatg cggtgatctc cgaagggatt cccacggcga tcgctctgga
     7141 gaccggggtc gcgacgctcg gcaatatgga ggcggtgcag tttcatccga ccccggcctt
     7201 tcctcccggc atcctgctca ccgaaggcgc ccgcggggat ggcggattac tgaaagacgt
     7261 agacggtcat cgtttcatgc cggactacga gccggagaaa agagagcttg cctcgcgcga
     7321 cgtggtctcg cgccgaatgg aagagcacat cgctaaaggt aagggcgtca aaacaccctt
     7381 cggcgatcat ctctggctcg acatcacgct tttgggcgaa caccacatca agcataacct
     7441 gcgcgaggtc tacgagatct gccattactt tctcggcgtc gatccggtga aggagtgggt
     7501 gccggtacgg ccggcgcagc actacacgat gggcggagtg cgcaccaacc acaccggcga
     7561 aagcccgacc ttgaaggggc tgttttcagc cggcgaagcg gcctgctggg acatgcatgg
     7621 ttttaatcgc ttgggcggca actcggtggc cgagacggtg gcggccggca tgatcgtcgg
     7681 cgagtttatt gctgacttct gcgaacgttc cgagaacgct gccaatttcc ctgccggtat
     7741 ggtccaggaa acactgcgcc gcgagcaagc gaagatcgac gtaatgatcg atggccgcgg
     7801 tacggaaaac gcggccgcgc tcatggccga gatgcagacg atcatgacca acaaggtcgg
     7861 catctttcgc agcggccgcg ttttggaaga ggcggtccac gaactgcagc agctcttgtt
     7921 gcgcagccgc aacatcggcc tgcgctataa agcgcgcggc gccaatccgg agctggtaac
     7981 ggcctatcgg gtgcagaaga tgctcaagat ggcgcagtgc gtcgcctacg gcgcgctgca
     8041 gcgaaccgaa agccgcggcg ctcactatcg cgaggacttc ccgcggcgca acgacgccga
     8101 gtggctgaac cgcaccttgg cgacgtggaa atcggaaaac gacaccctgc cgtcgctcgc
     8161 ctacgagcag ctcgatgtca tgaagatgga gctgccgccg ggctggcgcg gctacggcgc
     8221 caaggatcac atcgatcatc ccgatacgtc caggcgcctg gccgagattg aagcggtcaa
     8281 ccagggacgc gacggcgccg atcgtttctc cgtgcaacag aagctgatgc cctacgaaca
     8341 tctgttgccc aaacctttgc gcggcaggaa tgaacggatc gacgaaagga gaaccgagcg
     8401 tacctcgcct gcaggtctaa gagcgggatg aaacgcttct tagaacgcgg aaccggactt
     8461 tctgaccttg aactgctctg gaggagcgcg agaaaggagc atcgccatgg ctgagactcg
     8521 gaacgaaaga gatacgatgg gtgaaattgc ggtgccgtcg gaccgctact ggggcgcgca
     8581 gacgcagcgt tcgcttaaat attttcaggc gggcgccggg cgggacacca tgcccaggga
     8641 gctcatcaag gcctgcggca ttctgaaaaa ggctgcggcg atggccaacg tcgagctcaa
     8701 acttctggac caagaaagag gcgatctgat cagccaggtc gccgacgagg tcatcgccgg
     8761 caagctcgac gagcacttcc cgctggtgat ttggcagacc ggctcgggca cccagaccaa
     8821 catgaacgtc aacgaagtca tcagcaaccg cgccatcgaa atcgccggcg gcgtaatggg
     8881 aagtaagaaa ccgatacatc ccaatgacga cgtcaacaag gcgcaaagct ccaacgatac
     8941 ttttccgacc gccatgcaca tcgccgcggt ggaggttttg gcgagtctgg tcgaggcgtt
     9001 gaaggatttg aagaagcacc tcgatgacaa ggcgagcgcc tatgcgaacg tgatcaagat
     9061 cggccgcacc cacttgatgg atgccacccc gctaagcctc ggtcaggaga tttcgggttg
     9121 ggcccagatg ctggagaaca acctacaacg cattgatgca acgctgccgc acctttatca
     9181 actcgcgttg ggcggcacgg cagtgggcac ggggttgaac actcatccag agttcgctga
     9241 aaaagctgcg gcgaagatcg cggcgctgac caaccggcct ttcgtcagcg cgccgaacaa
     9301 gttcgaggcg ctggccggcc acgacgcgct cgtctttgcc cacggcgcgc tcaaaacgct
     9361 cgcctgctcg ctcatgaaga tcgccaacga cgtgcgttgg ctggcctcgg gtcctcgggc
     9421 ggggctaggt gaaatcagca tcccggagaa cgaaccgggc agctccatca tgccggggaa
     9481 ggtcaatccc acccagagcg aggccctgac tatgatctgc gcgcaggtcc tgggcaatga
     9541 tgtggccatc aatgtcggcg gggccagcgg caacttcgag cttaacgtct acaagccgct
     9601 gatgattcac aacttcattc agtcggccaa gcttttgtcc aagggtgtgg cgggcttcac
     9661 gcagcattgc atcgctggtc tcgagcccaa cgccgatcgc atcgacgagc tgatgaagaa
     9721 aagtttgatg ctggttaccg cgctcaatcc gcacattggt tacgacaacg cggccaagat
     9781 cgccaagaag gcccacgccg agggcacaac gctcaaagaa gccgcattgg cgctgggcct
     9841 ggtgaaagct gaggatttcg acaagtggat caagcccgaa gaaatgatca cgccgggaat
     9901 gtgaagatct taggtctcga aagaaaagca tcgaaaaccc tatcggagga cagagatgaa
     9961 aaatgaaacc gcgcggcgca cgctgaagtt taacattctt cgctttaacc cgcagg
//
LOCUS       DASUVS010000312        17611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1440295, whole genome shotgun sequence.
ACCESSION   DASUVS010000312 DASUVS010000000
VERSION     DASUVS010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 17611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17611
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440295"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      350..434
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            414..3338
                     /locus_tag="VF182_16795"
     CDS             414..3338
                     /locus_tag="VF182_16795"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX6438798.1"
                     /translation="MKPATEILCRFFFAKKPVRSGVLASEILEDQMNNDVQSPSTYRR
                     RNSPKRLIWCFVLLANLLYLPLASSQIVGTGQLTVERRSHSATLLENGKVLIVGGDNQ
                     NGIVSQAEIFDPISQTSALAAALIAPRTDHSATILSDGSVMVIGGRDQNSSLQSTEIY
                     KPLTTSFVAGPAMMRARSGHTATILANDKILVTGGDLFGSAEIYDPATQSFSLVPGTL
                     NIPRKFHSAILLQNGQVMIVGGVNAQEAMLNTAEIFDSASQTFYLPPTDMQTARAFAT
                     LKLLSDGKVQIIGGDAELSMEVFNPSNGIFIAKALLPPNPDLLGATLSTHSRAALFSP
                     TIAQDPLLQGILTAEQLSLLDRADQSITELPSRNQALVAGGVKSTGQVLNSAKLVSSS
                     KASVTTDKTDYAPGQIVTITGSGFQPNEQVHIYFHEFPEEYPDVFLSAVTNQQGGFVV
                     AEFAPQEIDVGRVFTLTALGQSSGFTAQTAFKDNKNLTLTKAGTGTGTVTSTTSSVGG
                     GSINCGVTCTSVVISFGDTDTVTLTAAPTSGSVLVGWSFSSSGGDGPPSSPSAGCSSA
                     GTTCTFGMGNKKQEVTATFNAAAAVTTTAINAPTVTYGTNGSVTVTVSSAVGTPTGNV
                     TLSVDGGTPVSKVLSGGSAIFTSTDITALTSPNAGDHSLSASYSAQGNLAASAATGNL
                     HVNKASSTTVVTFETGPYTYRGTAFTATAVVTGVGGLNQTVAVVYSGDCTNVTSANGC
                     TATASFAGDTNHNGSNDSKSITINKRSITVTADAKTKTYSESDPPLTYQVTSGSLALS
                     DAFTGALSRVAGELVGTYAIQQNTLSITNVSNYILTYIGGNLTITTGFAFNGFQSPIG
                     GSVENADGGTYANPVKSFKLGSTIPVKFAASWLNDGAALISGIHTLKAIKYSNSTDSD
                     PPIDATPTDAATTGNQFRLTSTDWHFNLSTKGNGFTAGTWLLSATLVDGSQHTVWISI
                     KK"
     gene            complement(3744..5873)
                     /locus_tag="VF182_16800"
     CDS             complement(3744..5873)
                     /locus_tag="VF182_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX6438799.1"
                     /translation="MSQQFAEKIGDLLTQEGLLTAEQLAKSVAIQKQQASAQPIGKVC
                     VSLGFVTPIDLEKVLVKHRRRIPLGELLVHLGLLTPGQVQSALEQQKKLNPHKRLGAV
                     LIEKGYINEAALIRALYEQSEPANSDKRKQGKFDALVATRRLSQRDLDQALREAQIQQ
                     RPAEALLMERYKLKKQELGAALSVFYGCPFKEYDEKQPPAWDSIRGLNLNYLRAASWI
                     PLQVSERSVEVLIDDPTAYEKIQDIKRLFLGKQIKFAVGLRDDILRYFSTAKANLEKN
                     HSHETITTILGQLDIAAHDEADDDSLNDPAINENHSAIVRLVNQIITDASKKRVSDIH
                     IEPYGSQNDTEVRFRVDGHCSNYLRVPAVYRRALVSRLKIMAGLDIAERRKPQDGKIK
                     FRLPDREIELRVATIPTAGIGNEDVVLRVLAAGEPIPINELRMTSRNLNKFRELLAKP
                     YGLILCVGPTGSGKTTTLHSALNVINTAERKIWTAEDPVEITQRGLRQVQVNSKIGFN
                     FAAAMRSFLRADPDVIMVGEIRDRETAEIGIEASLTGHLVLSTLHTNSAVETITRLLE
                     MGMDPFNFADALLGVLAQRLARMICASCKEPYQPAKEELDGLAYGYGEAAFAQLEIAQ
                     KENLVLYRGKGCAECNQSGYKGRIGLHELLVVSDEIKRLIHARATVAELWQVAVGQGM
                     TTLVQDGVQKVLAGCTDYGQVKAAATR"
     gene            complement(6301..6966)
                     /locus_tag="VF182_16805"
     CDS             complement(6301..6966)
                     /locus_tag="VF182_16805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6438800.1"
                     /translation="MEHLGDYQNCTILYVDDEEKSLVNFNRVFRNKFRILSAANAAEG
                     YRLLEEYRDDIALLMSDQRMPGEKGVEFLKRAREVHPNAVRILTTAYSDFDVAIEAMN
                     SGAVSKLITKPWDIPQLEMILKEACDFFTAQHESESPPDTDFSAPLKATPHERGAEIG
                     ELRTLEQVARYLNVDKFTVYRLITQKKIPAFKVGNQWRFKQEMIDAWLLEKANIKRTR
                     SDF"
     gene            complement(7682..8536)
                     /locus_tag="VF182_16810"
     CDS             complement(7682..8536)
                     /locus_tag="VF182_16810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6438801.1"
                     /translation="MPFANVRDAHINYKILGTHGPWVALSPGGRRDISAIEPLAQHVA
                     NAGFRVLIMDRRNCGAADVVIAGENSEYEIWADDFHELLSQLGALPAFIGGSSSGCRT
                     SLLFALRHPEAVRGLLLWRVTGGRFACERLAQEYYGQYISAAKERGMAAVCEMEHWKE
                     RIAARPANRDRLMNMEPSQFIEVMAHWREYFVLGADLPVIGATETELKCIDAPALVIP
                     GNDNTHSRKTGERLARILPNAQVHVLFPKHYDMDLSPREEWDEKAPEMARLFAEFMTA
                     SKPEDSKA"
     gene            complement(8669..9691)
                     /locus_tag="VF182_16815"
     CDS             complement(8669..9691)
                     /locus_tag="VF182_16815"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6438802.1"
                     /translation="MILKTGLVFAAVLILTASKNELFAAEPAFEGKAIRIVVGFSAGG
                     GFDTYSRAIARHMGKHIPGKPSMVVENMTGAGSIVAANHLYRIAKPDGLTIGNFHGFQ
                     ILNQVLGAPGIEFDARKLEWLGVPVQDTGACALTKASGIGTIEQWQAAKQPVKLGGVG
                     PGDASYGMARVLKEVLGLPVQPIPGYKGTADIRLAADTGEIAGGCWQWESIKVIWRQG
                     IETSDANVVLQLVPKPHPELSKVPLAVSLAKTEEARTLLQAAVHDPNSITRPYSLPPG
                     TPKDRVKILQKAFMDTMKDPEFLTDAKKSQLDIDPLAAEEVEKTVARFFNLDAALVAK
                     LRDILK"
     gene            complement(9790..10158)
                     /locus_tag="VF182_16820"
     CDS             complement(9790..10158)
                     /locus_tag="VF182_16820"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6438803.1"
                     /translation="MSRIRHIAITTKDVARLVNFYTTVFGLKIVPGRGTGTYLSDGHV
                     NLAILPLEPERETEGPYLKEGFYHFGFQVDDVDALRPISEEMGATSKIEKRPDNREVE
                     ARLYDPDGNPVDLSKRGWPI"
     gene            10639..11664
                     /locus_tag="VF182_16825"
     CDS             10639..11664
                     /locus_tag="VF182_16825"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6438804.1"
                     /translation="MREYRRGIWLALLLCLTFCSVAPAAEDFYSGKTIRLIVATTPGG
                     GFDAYSRMIARHMGKHIPGNPTFVVENMPGAAFMIGTNYLYKQAKPDGLTLGNWIGTL
                     VLHQVIGKKGIEFDARKFEYVGAPVKNHDTCVMSRASGIDSAKKWLSSPMPVKLGATP
                     PGSTPYDNAAVLKEALGLPMQLVSGYKGTAEIRLALDGGEVSGLCGLAWASTKVTWRK
                     QLESGEIKVVLQNTAVAHPELPDVPTAISFAKTDASRKLIELALHDVGAITYLYTFPP
                     GTPKDRLQLFRQGFQATLKDPEFITEAKKGNFELDPVPGEELEKIVNGFFKADAATIN
                     RLRDLLK"
     gene            12155..12301
                     /locus_tag="VF182_16830"
     CDS             12155..12301
                     /locus_tag="VF182_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438805.1"
                     /translation="MTNDREIELEMVSAKTPKIRTVAKQGIFFKDRATTTEIIMQTER
                     VLRV"
     gene            12560..12988
                     /locus_tag="VF182_16835"
     CDS             12560..12988
                     /locus_tag="VF182_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438806.1"
                     /translation="MNSRIKANVVIIVLILTSALTGAYAFAGDRRHPEHFLPEERRII
                     NEYYRGTNTTKKGLPPGLAKRGGKLPPGLQKHLEKDGQLPPGLQKRLEPLPSALDRRL
                     PSIPEYWERVIIEHDVILLDRRTNRILDIIENVIGLASGH"
     gene            complement(13134..13298)
                     /locus_tag="VF182_16840"
     CDS             complement(13134..13298)
                     /locus_tag="VF182_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX6438807.1"
                     /translation="MNQVVAFVVLSISMLTLAGCEIVGNIFEAGVWVGVILVVVIIGV
                     VVWLFTRLRT"
     gene            14842..15252
                     /locus_tag="VF182_16845"
     CDS             14842..15252
                     /locus_tag="VF182_16845"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEX6438808.1"
                     /translation="MKNVDVVAGVIVQERLLLVCQRKESAAFPLKWEFPGGKVEPDEA
                     HEDALRRELMEELGIKAGKLTEIFSHTHKYSDEFIVNLRFYRVHNFTGALTNRAFQDL
                     AWTGIDEVSRFDFLDGDLPFVDLLVSASGRKFLA"
     gene            15342..15968
                     /locus_tag="VF182_16850"
     CDS             15342..15968
                     /locus_tag="VF182_16850"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX6438809.1"
                     /translation="MIKKTLIPAFVLVYHLSAVPIALADVYVHKDKDGVLTFTNVPNH
                     SGFRRVIRESIAPLPRQTYTLKNFPAKSFDDFIISASTRHSIDADLIRAVIKTESDFD
                     ANARSSKGAMGLMQLMPETARQHNVLDAYNPFDNIEGGVRHLRLLLSRYRGNLELSLA
                     AYNAGINAVERHGGIPPYAETRQYVQRVLHFYDGYRENGVQNVLQAHR"
     gene            16005..16580
                     /gene="pgsA"
                     /locus_tag="VF182_16855"
     CDS             16005..16580
                     /gene="pgsA"
                     /locus_tag="VF182_16855"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX6438810.1"
                     /translation="MVWTFPNFLSFIRILIIPLLVYLLTRTDPTSSLIAAVLYLLASI
                     TDYFDGYLARRNKSVSDVGKILDPLADKLMVASALIMLAAMDRPGEPSVPAWLVVIII
                     ARETAVTIIRGIALTEGIVMHAEALGKYKFILQSFAVVGLLVHYAYWGVDFYTAGIYF
                     LTLAAVLALWSGVNYHLQFFRLRQSRVSAHS"
     gene            16625..16701
                     /locus_tag="VF182_16860"
     tRNA            16625..16701
                     /locus_tag="VF182_16860"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16659..16661,aa:Gly,seq:gcc)
BASE COUNT         4126 a         4574 c         4645 g         4266 t
ORIGIN      
        1 cggcctatct taccgcctgt tgagccgatc gcacctcctc aaattctcag attttctctc
       61 cagtcaatgg ggcgcagtcc aattttcaaa cacacctttt gccgcccggc actttccgga
      121 tgattagatc catacttcta gccgtatcag cttgttctga accactggat ccatcagctg
      181 tcacgccatt tccgacatga ttgacattcg atcggcgtca caactttgcc tagctcgaag
      241 aaagcattct agtcagaaga tgaccaggaa tgacatcggt tgaacgccta gttggattcc
      301 tctgccttcc atttcgtggc acacgtcatg ctctaccgcc gcagactcat aagaaagttg
      361 acgcgatgaa ctgattgccc cacgtggtca ccacgggcgt cagggagcga atggtgaaac
      421 cggcaacaga aattctgtgc cggttttttt ttgccaaaaa accggtacgg agcggcgttc
      481 tggcaagcga aattttggag gatcagatga acaacgatgt gcaatcgccg agtacgtacc
      541 gtcgccgaaa ctcgccgaag cggctaattt ggtgttttgt gcttttggca aatctgttgt
      601 acttgccatt ggcttcttcg caaatcgttg gaaccggcca actcaccgtc gagcggcgca
      661 gccatagtgc tactctgctc gagaacggaa aagttttgat cgtcggtggc gataatcaaa
      721 atggaatcgt gagtcaggcg gagatttttg atcccatctc gcaaacctct gctctcgcgg
      781 ctgcgttgat agcacccaga acggatcact ctgccaccat actgtccgac ggtagtgtaa
      841 tggtgatcgg cggtcgcgat cagaacagtt cgttgcaatc cacggaaatc tacaagcctt
      901 taacaacgtc tttcgtcgct ggtcccgcga tgatgcgcgc gcgcagcggc cacaccgcta
      961 cgattctagc gaacgacaaa attcttgtca ccggcggcga tcttttcgga agcgcagaga
     1021 tttacgatcc ggcaacgcaa agtttttcac tcgtaccggg aaccttgaat atacctcgca
     1081 aattccacag cgccattctg ctacaaaatg gtcaggttat gatcgtaggt ggtgtcaatg
     1141 cgcaagaggc gatgctcaac acagctgaga ttttcgattc tgcttcgcag actttttatc
     1201 tcccgcccac ggatatgcag acggctcgcg ccttcgcgac cttgaaactg ttgtcggacg
     1261 gcaaggtaca gattatcggc ggcgacgccg aattatcgat ggaggtcttt aatccgagca
     1321 atgggatttt tatcgcaaaa gcgctcttgc cgccgaaccc agatctgctc ggcgcaacat
     1381 tgagcactca cagccgcgcc gctctctttt ccccgacaat tgcgcaggat ccgttactgc
     1441 aaggaattct tactgccgaa caattgtcgc tgctcgaccg cgccgatcaa tcgattacgg
     1501 aattgccatc gcgcaatcaa gcattggtgg cgggcggcgt gaagagcacg gggcaagtgc
     1561 tgaactctgc caagctggtg agtagctcta aggcaagtgt taccacggac aaaaccgatt
     1621 acgctcccgg ccaaattgtg acgatcaccg gctccggttt tcaaccgaac gagcaggtgc
     1681 atatctattt tcatgaattt cctgaggaat atccggatgt ttttctctcc gcggtcacaa
     1741 accagcaggg tggcttcgtg gtcgccgaat ttgccccgca agagatcgac gtcggaaggg
     1801 tgtttaccct aaccgctctc ggtcagagct caggcttcac agctcagacg gcgttcaagg
     1861 acaataagaa tttgaccctg acaaaggctg gtacgggcac ggggacggta accagcacta
     1921 catcttcagt gggcggtggc agcatcaact gcggagtcac gtgtacctcg gtcgtcatat
     1981 catttggcga tacggacacc gtaacactga cagcggcgcc aaccagcgga tctgtcttgg
     2041 tggggtggag tttttcaagc agcgggggtg atggtcctcc tagcagtcct tccgccggtt
     2101 gtagtagcgc aggcaccacg tgcacctttg gcatgggtaa caaaaaacag gaggtgaccg
     2161 cgacgttcaa cgctgcggct gctgttacga caacggccat caatgcacca acggtcacct
     2221 acggcaccaa cgggagcgtg acggtaactg tttcgtccgc ggtaggaacg cccacgggga
     2281 atgtaacgct ttcggttgat ggcggtactc cggtttcgaa ggttctgtcg ggtggatcgg
     2341 cgatattcac cagtacggac attacggcgc tgaccagtcc gaatgccggg gatcacagcc
     2401 tgagcgcgag ctattcggcg cagggaaacc ttgcggccag tgcggcgacg ggaaatctgc
     2461 acgtgaataa ggcgagctcg acaacggtgg tgactttcga gacgggtcct tacacctatc
     2521 gcggcactgc ctttacggct actgcggtgg taaccggtgt gggcggactc aaccagacag
     2581 tggcagtggt ctactcaggc gactgcacca acgtgaccag cgccaacggc tgtacggcca
     2641 cggcaagctt tgccggcgat accaaccaca acggcagcaa cgacagtaag agcatcacga
     2701 tcaacaaacg ttcgatcacg gtgaccgcgg atgccaagac taagacctat agtgagtctg
     2761 atccgccgtt gacctatcag gtaaccagtg gatcattggc tctaagcgat gccttcacgg
     2821 gggcattgag cagagtagcc ggggaactcg ttggcacgta tgcgattcag cagaacacgc
     2881 tctcaatcac caacgttagc aattacattc tcacctacat aggaggcaac ctgaccatca
     2941 cgaccggctt tgctttcaac ggtttccagt cgccgatcgg tgggtcagta gagaacgcgg
     3001 atggtggcac ctatgccaat cctgtcaaat cattcaaact gggcagcact ataccggtaa
     3061 agttcgcggc gagttggcta aatgacggcg ctgcattgat tagcggcatc cacacgctga
     3121 aggccatcaa atacagcaat agcaccgact cggacccgcc gatcgatgcg acgccgaccg
     3181 atgcggccac caccggaaac cagtttcggc tgaccagtac ggattggcac ttcaatctca
     3241 gcacgaaggg gaacggattc acggcaggca cctggttatt gagcgctact ctggtggatg
     3301 gtagccaaca taccgtctgg atttccatca agaagtaacc ccaacctcag aaccagccaa
     3361 accgacagtc gccccacaaa gggcggctgt cgttgtattt ggaaatacga agaactctag
     3421 catgcgggct cgggtgtata gtctcggatt gcctgcatgg tggaaaaata gtgagaaccg
     3481 cctatcacct aacacggggt ctagagagac atcttgccaa agtagaccgt aggtggcgca
     3541 atcagacgat tcggctcgcg acaggaacca acggtactcc aggagtgagt gtcacttcgc
     3601 tcaaaacgtt gcactagtca attgtacagc gtcgtagtgg cttcgccgcc gcagtctcga
     3661 acctgggata gcgtcatcaa gaatgcttta atcataggtt ataaacggag tcccaggatg
     3721 catctgtgcg gcgtactggt tcactatcgt gtcgccgcgg ccttgacttg cccatagtcg
     3781 gtgcaaccag ccagaacttt ctgcaccccg tcctggacca gggtcgtcat tccctgcccg
     3841 accgcgactt gccagagctc cgctacggtc gcgcgcgcat gaatcagccg tttgatttcg
     3901 tcactgacaa ccaagagctc gtgcaaaccg atgcggcctt tgtaaccgct ctgattacac
     3961 tcagcgcacc ctttgccccg gtacaatacg agattctcct tctgggcaat ttccaactgc
     4021 gcaaaagcgg cttcaccata cccgtatgct aacccgtcaa gttcttcttt ggcgggttga
     4081 tacggttctt tgcagctggc acagatcatc cgcgccaacc gctgcgccaa cactcctaac
     4141 aaagcatcgg caaaattaaa cggatccatc cccatttcta acagccgcgt gatggtttcg
     4201 acggcgctgt tagtgtgaag ggtgcttaat accagatgac cggtaagcga agcttcaata
     4261 ccgatttcgg cggtctcgcg atcgcgaatc tctccgacca tgatgacatc cggatcggcg
     4321 cggagaaacg agcgcattgc ggcagcgaaa ttaaacccta tctttgagtt gacttgaacc
     4381 tggcgcagcc cgcgctgggt aatttcgact ggatcttctg ctgtccagat tttgcgctcc
     4441 gctgtattga tgacgttaag ggcggaatga agggtggttg tttttccaga tccggttggg
     4501 ccgacgcaca aaatcaaccc gtacggtttg gccaacagct cgcgaaattt gtttagattg
     4561 cgcgaagtca ttcgtaattc gtttatcgga atcggctccc cggccgcgag cacacggaga
     4621 actacatctt cattgccgat gccggccgtg ggaatagtgg ccacgcgtaa ttcgatctca
     4681 cgatcgggca ggcggaactt gatcttgccg tcttgcggct tacggcgttc ggcaatatcg
     4741 agaccggcca tgatttttag cctggaaacg agggctctgc gatacaccgc cggaacgcgc
     4801 aaataattag agcaatggcc atcgacgcgg aagcgcacct cagtatcgtt ttggcttccg
     4861 taaggctcga tgtgaatatc ggagaccctc tttttgctgg cgtcggtaat aatttgattc
     4921 accagcctga cgatggcgct gtgattttca ttgatcgcgg ggtcgtttaa gctatcgtca
     4981 tctgcctcat catgcgcggc gatatcgagc tggccgagaa tcgttgtgat cgtttcgtga
     5041 gagtgattct tctcgaggtt agccttagcg gtgctgaaat acctcagaat gtcatcgcgc
     5101 aagccaacgg caaacttgat ctgtttccct aaaaacagcc gcttgatgtc ctggattttc
     5161 tcgtatgctg tggggtcatc gataagcact tcgacggaac gctcgctaac ctgtaaagga
     5221 atccaagaag cggctctaag ataattcaaa ttaaggccgc gtatggaatc ccaggccggc
     5281 ggctgtttct catcgtactc tttgaaggga cagccataga atacgcttag cgccgcaccc
     5341 agttcttgtt ttttcagctt gtagcgctcc atcaaaagag cttcggccgg ccgctgctga
     5401 atctgcgctt cccgaagcgc ctggtctaga tcgcgttggc tcaaccgccg ggtcgccacc
     5461 aacgcatcga actttccttg tttacgtttg tcggagttcg cgggctcaga ctgttcataa
     5521 agcgcgcgaa tcagcgcagc ctcattgata taaccctttt caatgagtac cgcgcctagt
     5581 cgtttatgcg ggtttaactt tttttgctgt tcgagagcac tttgtacttg acctggggtg
     5641 agcagcccga gatgaaccag aagttcacct agcgggatcc ggcgccgatg tttaaccaaa
     5701 accttttcta aatcgattgg tgtgacgaac ccgagcgaaa cgcagacttt gccgatcggt
     5761 tgcgcggaag cttgctgttt ctggatagca acgcttttgg caagttgttc tgcggtgagc
     5821 agtccttctt gagtgaggag gtcaccgatc ttttcagcaa attgttgaga catcgatcgt
     5881 tcgaagggag tattagccga ccgagcaagc agtataccaa ggttaaagca ttgaaaagga
     5941 acacccttgt cgactcatca cccgcgaagg aagcgcagga tgaacaaaaa tgtcaattta
     6001 tgtcaagcgg tgtgcttgcg tatctgctgc ctcgggggca tgaattacca aattctgccg
     6061 agaagcatca actgtggtcg taactcttcc ttattgtgcg ggatttggct ctgtaagata
     6121 gcatctggcc gatgtcaccg aaccgatccg gaacaagcag cggcagacaa ggaacgggtt
     6181 attgatggaa ttgaatcgtc ggattcttcg atcagcaaag atggagtcca tgggtcggct
     6241 gtattgactc ttaggagttg ccctcgacgc cggcaaaaga gtggtaacga aaactcgtgc
     6301 ttagaaatct gatctcgttc ttttgatgtt tgccttttcc agcagccagg cgtctatcat
     6361 ctcttgcttg aaacgccact gattacctac cttgaacgca gggattttct tctgcgtgat
     6421 gaggcgatag acggtaaact tgtccacgtt taaataacgg gccacttgct ccagcgttct
     6481 tagctcaccg atctcagccc cacgctcgtg aggtgtcgct tttaggggtg ccgagaagtc
     6541 cgtatccggc ggagattcgc tttcgtgttg cgccgtaaag aaatcacatg cttctttaag
     6601 gatcatctcg agctgaggta tatcccaggg tttggtgatg agtttggaaa cagcgccaga
     6661 attcatcgcc tcgatcgcca catcgaaatc tgagtacgca gtagtcagaa tacgaacagc
     6721 attcggatgt acttcacgag cccgctttaa aaattcgacg cctttttccc ccggcatccg
     6781 ttgatcgctc atcagaaggg cgatgtcatc tcggtattct tcgagcaggc gatagccctc
     6841 ggctgcattg gcagcgctta gtattctaaa cttgttgcga aacactcgat tgaagttgac
     6901 gagggatttc tcctcatcat ctacatatag aatagtgcag ttctggtaat cgccgagatg
     6961 ttccatcgtc tctcctctca agccgagaaa cttctggtca ttcgtaatgt ggaatcggaa
     7021 ttgccgttgt tatgtcgtgg cgtcggatcc acccgaaagc tgtttcatct aaattagcag
     7081 atccttagcg ccagtctaca cttctcttct cgccgtagtg ccgggcgacg ccagtaagtg
     7141 atttaaacgt tgggcgcatc ctctatcccg ccgccgcgca caagcacaaa aagaatctgt
     7201 ttcggcagat taataaaccc cgtccttgcc gggcgacttt ggttcaacaa aggtcagctg
     7261 tatcgaatgg ctggcccacc atatcagatc atctagggtg ggcgttttcc aaacccgcgt
     7321 gctcttggtt cgagatttgc ccaccggtgt ccctagagta gatctgcaat ggaaatgcca
     7381 gacagtttca ggcatttggg ctgtactacg gatgactgca acatctcctt tattgctgat
     7441 tagcttacag tcgcgaaaat gggccgccat agcaaggcat gctcgacggg aattcctata
     7501 gatgcacgtt ttctttggat cttgtgccga tcgtggacga cgccggaacc ggcagtcgtg
     7561 cctcccgata tcggttagtc atccataacc gaactgtacg aagcgactgc gctcttcaga
     7621 aaacgtgcga tttggtccca agtctcggaa ataacggtca tgtttggact ttagaatcgc
     7681 tttaagcttt agaatcttct ggtttactag ccgtcatgaa ctcggcgaaa agtctggcca
     7741 tttccggagc tttctcgtcc cattcctctc ggggacttaa atccatgtca tagtgtttag
     7801 ggaataagac atgaacttgg gcatttggaa ggatgcgggc caagcgctcg cccgtcttcc
     7861 gcgagtgtgt gttgtcatta cccgggatga cgagcgccgg tgcatcaata catttaagct
     7921 ccgtctcggt tgcgccgata accggcaggt cggcgccaag cacgaaatac tcgcgccaat
     7981 gtgccattac ttcaatgaat tgggaaggct ccatattcat gagcctatct cggttcgccg
     8041 gccttgctgc aatgcgctct ttccagtgtt ccatctcaca aacggcggcc attccgcgct
     8101 cctttgcagc gctgatgtac tggccataat attcttgggc taaacgttca catgcgaagc
     8161 ggccgccggt cacgcgccag agtaagagac cgcgtaccgc ctcgggatgg cgtagggcaa
     8221 aaagaagcga ggtgcggcaa ccggaagagc tgccgccgat gaaggcggga agagcgccca
     8281 gttgtgagag caattcgtga aaatcatcgg cccagatttc atactccgag ttctcaccag
     8341 cgatcaccac atcggcggcg ccgcagttgc gacggtccat gatgagaaca cgaaagccgg
     8401 cattagctac gtgctgggcc agcggttcaa tcgcgctgat atcacggcgc ccgcccggtg
     8461 acagtgctac ccatggacca tgagtcccga ggattttata attgatgtga gcgtctcgta
     8521 cgttggcgaa tggcatattt cctcctcccc tgcggtgatt aatcagtaac aaatggtcag
     8581 taggattttg accaactgta tattcaggat gattacaatc cgcacctctg aaccgattgg
     8641 ctacaataac aacccgttcc agatagcgtc atttgagaat gtcgcgcagc ttagctacga
     8701 gagccgcatc caggttgaaa aatcttgcga cagttttttc tacctcttcg gctgctagcg
     8761 ggtcgatatc gagctgggat ttttttgcat cggtaagaaa ctccggatcc ttcatggtgt
     8821 ccataaatgc tttttgcaat atttttacgc gatccttcgg cgtaccgggc ggtaacgagt
     8881 acggcctggt tatcgaattc gggtcgtgaa ccgccgcctg cagcaaagtt cgagcttcct
     8941 cggttttagc gaggctgacg gccagcggta ctttggaaag ctcgggatgt ggtttaggta
     9001 cgagttgaag cacgacattg gcatcgctgg tttcgatacc ctggcgccag atcaccttga
     9061 tcgattccca ttgccaacag ccgccggcga tctcgccggt gtcagccgcg agtcgaatat
     9121 ccgctgttcc cttataaccc gggatcggtt gaaccggcag gcctaacacc tctttgagaa
     9181 ctctcgccat gccataagag gcgtcgcctg gtcctacacc gccgagcttg accggctgct
     9241 ttgccgcctg ccattgctcg atggtgccga tgccgctcgc tttggtcaaa gcgcaggcac
     9301 cggtatcttg caccgggacc cctagccatt cgagcttgcg cgcatcaaac tcaattcctg
     9361 gagcgcccaa gacctggttt aaaatctgga aaccgtgaaa gttgccgatc gtaagaccgt
     9421 cgggtttcgc tattctgtag agatggtttg ccgcaacgat acttccagcc cctgtcatat
     9481 tctccacgac catggacggt ttgccgggaa tatgtttgcc catgtgacgc gcaatggctc
     9541 gcgagtaggt atcgaaaccc ccaccagccg aaaaaccgac gacgatccgg atcgcttttc
     9601 cttcaaatgc gggttccgcg gcgaaaagct cgttcttact agctgtcaga atcaaaacgg
     9661 cagcaaacac taaaccggtt ttcaaaatca tcatgagctc ctgaatcagt ctcgcatcac
     9721 gtaattggcg tcgaatcctg ttgggacgca aaacagttat tccaataggc tgtctaaggt
     9781 atgagtgcct taaatcggcc atccgcgttt cgaaaggtct acaggattgc cgtcaggatc
     9841 atagagacgc gcctcgacct cccggttatc cggccttttc tctattttgg aagtagcgcc
     9901 catttcctcg ctgatcggtc gaagtgcgtc cacgtcatct acttgaaagc caaaatgata
     9961 gaagccttct ttgaggtagg gcccttccgt ctcacgttca ggttcgagcg gcaaaatcgc
    10021 caggttaacg tgaccatccg aaaggtaggt tcccgtgccg cggcccggca caatttttag
    10081 accaaaaacc gtggtataaa agttgacgag acgagcgaca tccttggtgg taattgcaat
    10141 atggcgaatg cgcgacatag acttagcctc catccaggat ttagccttta tccagcttag
    10201 aatcttttct ccagtttgcc gattccttta cccatgcagc acatctagct cttagctcgt
    10261 tttcgagaac taccataggg tcaacgaatg atcagcgtca agccgtttgg cgcgcattca
    10321 cgcatcctat tcggcaaccg acctcatgga atcaaaggat gggatagcgg cagatctacg
    10381 tgatcctagc cgtagtattt ccgaattcga ctcatgtgct ggccttaacg ttataggtga
    10441 cttcatcaga gtatgcatga tgctgagctg cgccttttcc gcggaagagt ttctcgaaat
    10501 cgagctctgc gtatttccaa taccctccgg gtgttggggt gagttccgtt tctagaagta
    10561 ttttcttggt ggtggggcga tcatttgata gagagattcc attgatcaaa acttaaccga
    10621 actactcgga ggcgccctat gagagaatat cgtcgcggca tatggttggc gttattattg
    10681 tgcctgacat tttgttcggt agcgccggct gcggaagatt tttacagcgg caaaactatc
    10741 cgcctgatcg tagcaaccac gccaggcggt ggattcgatg cctattctcg tatgatcgcg
    10801 cgtcacatgg gtaaacatat tccaggaaat ccaacgtttg tggtggaaaa catgccgggc
    10861 gccgctttta tgattggcac caattatttg tacaagcagg ccaagccaga tgggctgacc
    10921 ttgggcaact ggatcggcac gctggtgtta catcaagtga taggaaagaa aggcattgag
    10981 ttcgatgcgc gcaagttcga gtatgtcggc gcgccggtta agaatcacga cacctgcgtg
    11041 atgagtcgcg ccagcggtat cgatagcgct aaaaaatggt tgagctcgcc gatgccggta
    11101 aagctaggcg cgacgccacc gggttcgacg ccttacgata acgcagcggt gctaaaggaa
    11161 gcgctcgggc tgcccatgca gcttgtctcg ggctacaaag gcacagccga gatccgctta
    11221 gccctcgacg gaggtgaggt gtcgggtctc tgcggtctgg cttgggcatc gacaaaagta
    11281 acctggcgca agcaattaga atcgggggag ataaaagttg ttttgcaaaa cacagctgtg
    11341 gctcatcctg aattacccga cgtgccaacc gcgatcagct ttgccaagac cgacgcgagc
    11401 cggaagctga tcgaactagc gctgcatgat gtcggagcca ttacctatct ctataccttt
    11461 ccgccaggta cgccgaagga tcgcttgcaa cttttccggc agggatttca ggcgacattg
    11521 aaagatccgg aatttatcac tgaggcgaag aaagggaatt tcgaactcga tccggttccg
    11581 ggcgaagaac tggagaagat tgttaatggt ttcttcaaag ccgatgcggc aacgatcaac
    11641 cgattacgcg atttgctcaa atgagcggcc gaggggaaga gcatgccgaa acgacgataa
    11701 atgcttcacg cttcaaagat tgacgcgact cgcctagtag catgtaggaa aattaccaag
    11761 ccaataacaa ttttgcttgt tccaactctt ctgatcttgt cccttcatgg ttaagaactc
    11821 ccatagctgg caattgccgc cacttatttc cagtggcgta tatgttgcct ttgatgttcc
    11881 cgcatggagc atcaaaggct accaatcttg ttcttagggc tattttcttt actttcactg
    11941 agcggttgcg cggctgtcgg aatgacgctc ttcggtggcg gagccggcgt tgctacgggt
    12001 tacttcagtc tcttccacgt aggacgcggg atcgcgtacc ggacatttac tgcctccatc
    12061 acaggtcgaa ggagctacac gtactgctct ggatcgtatg ggaataagat ccaagcgact
    12121 ttccaaaaca gagcagggta aggcgatcaa agcgatgacc aatgaccggg agatcgagct
    12181 tgaaatggta agcgccaaaa cacccaagat tcgcactgtg gcgaagcaag gcatcttctt
    12241 caaggatcgc gcaaccacga ctgagatcat catgcaaaca gaacgggtac tgcgagtctg
    12301 atggttaatt gcagtgtacc ggcacagttg gccgcgcctc ctccgcgcgg ccatgcgaga
    12361 tccggcccga gccgggaatt catcctcgcg gcgggatggc ggcgatcagc agaaacgtga
    12421 gatccgactg tagtgctgtc atagtttgtg ccatgcgagc ggagacaaag agcagatcag
    12481 aacgcgtgaa catcgaacgg aagccggcgt accaagagcg acaataagcg agtggggtat
    12541 cattaacaga ggagtggacg tgaactcacg gatcaaagca aacgtagtga taatagtcct
    12601 aatcttaact tcggcactta cgggcgcata tgcgttcgca ggagataggc ggcatcccga
    12661 gcactttcta ccggaagagc gcagaattat aaatgagtac taccgcggca ctaacacaac
    12721 caagaaaggg ttaccgccgg ggctcgcaaa gcgcggcggc aaattgcccc cggggttgca
    12781 aaagcattta gaaaaagatg gtcaattgcc tcctgggctg caaaaacgtc tagagcctct
    12841 gccctctgcg ttggatcgga ggttgccgtc aatcccggaa tactgggaac gagttatcat
    12901 tgagcatgat gtgatcttgc tcgatcgtcg gaccaatcga atcctggaca tcattgagaa
    12961 tgtgatcggg ctggcaagcg gccattagcg gtgactagcg gtgattgttc gattaagcca
    13021 gccccgtttt ctggtactct gaacgtttaa accacgacgt tataagatac tgctgtataa
    13081 ttcgcccgca gtttcgaagt tccaggaaat caccggacag cgtgctaacc ttctcaggtc
    13141 ctgagcctgg taaaaagcca gacaacgact ccaattatga ctacaaccag aattacgcca
    13201 acccacactc cggcttcaaa aatattcccg acgatctcgc acccggctag tgtcagcatc
    13261 gaaatagaca gcaccacaaa ggcgacgacc tgattcattg aaagcctcca gtctcggttt
    13321 cgaccgatat ctaaatgtat cagcacacca tgcgacaaaa caaaggccgc taagctttcg
    13381 cccagcgggc gtcagacgaa tgcttgcacg gagatacact atcgttagaa ggtgcgaaag
    13441 acgttcgttc tacacaccaa tccggggcaa ttggatcgcg tcatctttgt tcgaatccaa
    13501 agtttattct gcgcgggagg cgaattggac aagtcccagg agggacatgc agggttgtgg
    13561 agggagacgc cgtattgctt cgatgggcgt cctgatgctt tgctcgaata cggtgtccag
    13621 tgcctcccgc tggttttgca aaacgcaagt agcgggccga gcggtgtttc agttatcagt
    13681 aatgtgattg tgaactaaac atctacccag agcggccaag tgtgtcagat ccgccggatg
    13741 tggcagattt tttcgttcga tagaaggccg atggtcttga gccttttgat atgaaggtgc
    13801 ggcgtagccg cggctcgcga tcacgtatgg tgggaattat ttttgatcgg gacgaggcga
    13861 tgcttccgat gcgtaggagg aaaccggtgg tgtaaagctc ggtaaataat ctattttcga
    13921 gaagatgaaa attcccatca cgattccgag tacgataagt acttcggtta tagtatcccg
    13981 ctcaggcaca tagcgggcga gtccatttaa tagagttctc atgactggtc cattcctcac
    14041 gattggccat agaccgcaag atcggctcca aagacgcgca accgatattc taagctaatg
    14101 aaaagggaac gtttcaaatt tggaactttg acgcagctga tgtgccaaag atgcgacagg
    14161 aatcctatag gatcatttct ggttcaatct attcggtggt agatggctcg tccgttggag
    14221 aagtatctag ctacacgttt ccacgagtgc ttctggtttg aggggccttg tcctgttcct
    14281 gaatcacgca ttgactatac gccggtatga ttgctacaag aatcgagagc ctccgcttga
    14341 gtaacccata tgaggcacct gtagaattcg accaggatgg ccgtcaccct tatcccgtcg
    14401 aatacccagc acctatgcag acgctcgaat ttagcgccgc agtgtgcggg gagagctcag
    14461 gcattaggcg gatccgaact tgaactgcat cgggcgggga gtgcgaaaca agcacagaaa
    14521 tgccagctcc agtggaaagt tcagaaaaat gggtcgacga tggaccccgg cagccgattg
    14581 atcgacgctg cctgtggcgt caggttcgtc agaataccgt gaaattccga ctgtgcagat
    14641 ttcagcaagc tcctagaagt acaaccgtca atcgatcaga tcgccgcaaa taaatgggtc
    14701 acgagcgcgc ggatcaagaa ctagctccgc gagctgagaa aacctgctct ggctttctcc
    14761 ggctgtttag tgtccgggat ccgtcgcgca tacagactgt gatgtaatag tgatagctcg
    14821 gttacagcat acctagccaa aatgaagaac gtcgatgttg tcgccggtgt gatcgttcaa
    14881 gaaaggttat tgcttgtttg ccaacgtaaa gagagcgcag catttcctct caagtgggag
    14941 tttccgggtg gcaaagtgga accggacgag gcgcacgagg atgccttacg ccgcgagttg
    15001 atggaagaac tcgggattaa agctggcaag ctgaccgaaa tcttcagcca tacgcataag
    15061 tattcggatg agtttatcgt gaaccttaga ttctatcgag tgcataattt caccggggcg
    15121 cttacaaacc gtgcgttcca ggatctggct tggaccggga tcgacgaggt ctctagattc
    15181 gattttctcg acggcgacct gccgttcgtc gatctattgg tgtctgcttc aggccggaaa
    15241 ttcctcgcct gacggctcgc cgaagtagcg tttacgtcaa catcgaggtt tgacagcggc
    15301 acggcccatg ggtattctag cggcactttt caactccacg tatgattaaa aagacgctaa
    15361 ttccggcatt cgtccttgtc taccacttat cggccgtgcc tatcgctctg gcggatgtct
    15421 atgtgcataa agataaagac ggggttttga ccttcaccaa tgttccgaat cacagtgggt
    15481 ttcgtcgcgt gattcgtgag tcaatcgcgc ccttgccgag gcagacttat acattaaaaa
    15541 attttcccgc gaaatccttt gacgacttta tcatctctgc gtcgactcgc catagcatcg
    15601 acgccgattt gattcgcgct gtgatcaaaa cagaatcgga tttcgacgcg aatgcgcgct
    15661 cttctaaagg agccatggga ttgatgcagc taatgcctga gacagctcga caacataatg
    15721 ttctcgatgc ctacaatcca ttcgataata ttgagggcgg tgtccggcat cttcggttgt
    15781 tgctaagccg ttatcgcggc aatttggaac tgagtctcgc ggcatataac gccggaatca
    15841 acgcagttga aagacatgga ggtattcctc cgtatgccga aactcgccaa tacgtgcagc
    15901 gcgtgctgca tttttacgac ggataccgcg aaaatggggt gcagaacgtc ctgcaggcac
    15961 atcgataacg ctggtccgcg ccaaccttcc ttattgagct tcacgtggtt tggacgtttc
    16021 ccaatttctt aagtttcatc cgaattctaa tcatcccgct acttgtttat ttgctgactc
    16081 ggacagatcc cacttcgagc ttgattgccg cagtcctgta cttactggct tcgataaccg
    16141 attacttcga cggctatctg gcaagacgta ataagagcgt ctccgatgtc ggcaaaatcc
    16201 ttgatccgct tgcagacaag ttgatggtgg cttcggctct gatcatgctc gcagccatgg
    16261 accgtccggg cgagccgagt gtgcccgcct ggctcgtggt cattattatt gccagggaaa
    16321 ccgctgtcac gattattcgc ggcattgcgt tgactgaagg aatcgtcatg catgccgagg
    16381 cgctcggtaa atacaaattt attttacaat ctttcgcagt ggtgggtctt ttggtgcact
    16441 acgcttattg gggtgtcgac ttttatacgg cgggaattta ttttctaacg ctggcggccg
    16501 tgctcgcgtt atggtcgggc gtcaattatc acctgcaatt tttccgcctt cgacagtctc
    16561 gcgtctccgc gcatagctga tctaaattga caatatctct ggcttcatat aatttttaga
    16621 ttatgcgggt gtagctcagt tggctagagc gtatgcttgc caagcataag gtcgggagtt
    16681 cgaccctcct cacccgctcc atctttaagc ctccgatttt tcgggggctt tttcttttgt
    16741 gctgaggtag gggagtgctc gcggtcagaa gcaggtttca ctagggttgc acttttctgg
    16801 atctgctttt cgacaaactc ccggttgtca tcggcctctt tgatgtacct cgcatgatca
    16861 gtttaaagtg tctcgatatt atttccggtt tggctggaga tgaaatccag ctctggcggc
    16921 agctttaaat gcatccattg agcctcaagc cgttaagcgc ttggccttga ggttattctg
    16981 cagattctga actccgcgac agtcagagac cccggacaat ctaattgagg agtcggacaa
    17041 tcgacgcact gaggctggag tgcgaagcag ggacagtgtc agaactcgac aacggcaaac
    17101 ctgcgtgcct cgagggactc atggtctcgg cgctcggatg gacgacgcac tttagccccc
    17161 tgctgagcct agaatgcgtt attatagggg caaatgtctc cctttaggag gtcaggctaa
    17221 tgagctctag ctgggaccgt agaatcgata acacgaggcg gcggttcgaa gtcctcaacg
    17281 agtttggtgc cgacgcggtg ctggacaaga gactggcctt gtatgggaac gcagtcccag
    17341 caaagaagcc gtggcatggc aaaatgcccg ttcgacgctc gaaagcggta ggttaacgtg
    17401 tggatcgagg ctccctgcct tcaacgagtt agcgagtctc acaggtcctg cagtaacaga
    17461 tcccacggta ccacgacttc cagcggggca cccattcttg gatgttcagc cggcggcgtg
    17521 ttggtcttca acggtataaa gctgctttct ctttgcgtgc taacttcctg gcctatgaat
    17581 acttccactg cccggccact cgcggcactt t
//
LOCUS       DASUVS010000313         9130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_566397, whole genome shotgun sequence.
ACCESSION   DASUVS010000313 DASUVS010000000
VERSION     DASUVS010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9130
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566397"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /gene="purL"
                     /locus_tag="VF182_16865"
     CDS             complement(<1..1216)
                     /gene="purL"
                     /locus_tag="VF182_16865"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX6438811.1"
                     /translation="MLDQNASKKPPVTAELAASHGLTPDEYRKIIQILGREPNYTELG
                     VFSVMWSEHCSYKSSRRYLKSLPTEGPYVLQGPGENAGVVDIGDGLAVAFKMESHNHP
                     SFIEPYQGAATGVGGILRDIFTMGARPIASLNSLRFGRIDHPRTPYLVSGVVAGIGGY
                     GNCIGVPTVGGEVYFDECYDANIVVNAFTLGITKKKRIHTGLAKGVGNPVIYVGSKTG
                     RDGIHGATMASESFSEEKEQRRPTVQVGDPFTEKLLLEACLELMEHDHLIGIQDMGAA
                     GLTSSSTEMAARGGAGIELNLSAVPLREQGMTPYEILLSESQERMLLVAKAGTEAEIK
                     RIFDKWDLEAVVVGRVTADNEFRAVFNGDQVARIPISALTKEAPVYHRPAERPKSQDE
                     LEQLDLSTIAEPR"
     gene            complement(1209..1919)
                     /gene="purQ"
                     /locus_tag="VF182_16870"
     CDS             complement(1209..1919)
                     /gene="purQ"
                     /locus_tag="VF182_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX6438812.1"
                     /translation="MRWGVIVFPGSCDDQDVFDSLKREMDQKVKFLWHKDELVGHFDC
                     LVLPGGFSYGDYLRCGAMARFSPIMRGVEKFAQEGGLVLGICNGFQILCEAGLLPGAL
                     VRNAGLKFVCQQTWLRVEENDTPFTSQLKKGQLLKIPVKHGEGRYHADETTLERLKRQ
                     RQVLLRYVNAKGKTSPPANPNGSIDDIAGICNERRNVFGLMPHPELACNKLLGSDDGL
                     KIFASIAAACDLKSAAHA"
     gene            complement(2010..2255)
                     /gene="purS"
                     /locus_tag="VF182_16875"
     CDS             complement(2010..2255)
                     /gene="purS"
                     /locus_tag="VF182_16875"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006208285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX6438813.1"
                     /translation="MRVKIFVSLKKGVLDPQGKAVEQSLHSLGYKEVQDVRVGKFVEL
                     DLQAGSREAAEDRIREMCDKLLANPVIENFHYEISEG"
     gene            complement(2290..3582)
                     /gene="purB"
                     /locus_tag="VF182_16880"
     CDS             complement(2290..3582)
                     /gene="purB"
                     /locus_tag="VF182_16880"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX6438814.1"
                     /translation="MIPRYTRPEMGRIWSEENSFQKWLDIEILAAEALAQMGKVPKPA
                     ISRIRKNARFNVERIREIEREVKHEVIAFLSSVAESIGDDARFLHLGMTSSDVMDTAL
                     AIQLKEAAAILIEDVQSLMKVLRRQANRYKSTVMIGRTHGVHAEPITFGLKLALWYQE
                     TERNLVRLRKAVEDISVGQISGAVGTFAQISPKVEAYVCKKSGLKPALISNQIIQRDR
                     HAYFFATLAVIGSSLEKFAVEIRHLQRTEVQEAEEPFTPGQKGSSAMPHKRNPILSEN
                     VSGLARLMRSYALAALENVALWHERDISHSSVERVIAPDATIALDFMLRRMTYVLGAL
                     CVYPENMKRNLEKSGGAVFSERVLLALVEKGVSRDAAYRMVQRHALKTARDGGELKGD
                     LLKDQEIMSYLSASEVEAVWGLKHYLANVDFIFRRVFR"
     gene            complement(3647..4111)
                     /gene="dut"
                     /locus_tag="VF182_16885"
     CDS             complement(3647..4111)
                     /gene="dut"
                     /locus_tag="VF182_16885"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX6438815.1"
                     /translation="MEEIQIAIKRVRASGAPASLPSYMTEGAAGMDLCADLSHDLQLR
                     TLERRLIPTGIAIALPFGFEAQIRPRSGLALKEGLTLINSPGTIDADYRGEIQLIAIN
                     LGSDAIIIRPGQRIAQMIIQRVVRAMWQEVPELPQSERQEGGFGHTDALRKN"
     gene            complement(4141..5415)
                     /locus_tag="VF182_16890"
     CDS             complement(4141..5415)
                     /locus_tag="VF182_16890"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX6438816.1"
                     /translation="MFAKSVLDNGIRVVSQEMTDHRSVSLGIWVENGSRHELPDENGI
                     SHFIEHLLFKGTTTRTAAQIAEEMDAVGGLLNAFTSKEHTCYYAKVLDEDLPLASDLL
                     TDIFLNSNFDAEEIERERSVILQEISQAEDTPDDYVHDLFSLDFFPHHPLGRPIAGHV
                     GTVSKFRREDFVNFFHERYHPSRVVVAAAGHFQHDKLVTELNTRLGGSKKSVSAVPSR
                     VNAELLPQTRSGIFPHPKPLEQVHLCLGMPGIHQTHPQRHAGYILNTILGGGMSSRLF
                     QEIREKRGKAYSVYSFMSTYSDTGYLGIYAGTSLEAAEEVVDLVLKQLRCLAAGDINE
                     DEVRRTRGQLVGNIMLGLESTDSWMSHIARNEIYFGKSITTDEICQRIRSVTRDQIIE
                     LARALFRPEAMAVSLLGDFKDDFKIANFGLGQ"
     gene            complement(5439..7571)
                     /gene="pnp"
                     /locus_tag="VF182_16895"
     CDS             complement(5439..7571)
                     /gene="pnp"
                     /locus_tag="VF182_16895"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX6438817.1"
                     /translation="MIKKIELDFHGRPLSIEVGRLAKQADGAALVRYGETVVLATAVA
                     AKDFKLDTDFFPLTVDYQEKTFAAGKIPGGFFKREGRPSEKEILTCRLIDRSIRPLFA
                     EGLRCETQIIATVLSADRENDPDVVAMLGASAALEVSDIPFNGPLAGVRIGRIDRQLV
                     MNPTQSQLSDSDMDIFLSGSRAAIVMVEGGAQIIPEDEILEALFAGHEAIQPLLEMQE
                     EIRRAIGKPKRHVPLAELDQAAAGRVEGLALSKLRQALEVPEKLERYKRIAEVKSEVV
                     PLALAEHPDKAKDIKGAFEELKRNCFRNLVIEKERRIDGRGLKDIRPISCEVDILPRT
                     HGSALFTRGETQALVVTTLGTASDEQRVDALTGEHFKKFMLHYNFPPFSVGEVKFLRG
                     PGRREIGHGNLAERALLPVLPAEESFPYTLRIVSEILESNGSTSMASVCGGSLSLMDA
                     GVPVSAPVAGIAMGLIKEGEHVRILSDILGDEDHLGDMDFKVAGTAEGVTSLQMDIKI
                     SGVDRNIMGQALYQAKEGRMHILGEMGKALSTPRTNVSGHAPRIITIRVKPEKIREII
                     GPGGKVIRGIIEATGVKMDVEDDGTVTIASSDEAAAQKAIQMVQRIAAEAEVGKIYKG
                     TVRKIVDFGAFVEIFPGTDGLVHISQLAPERVRRVADVLKEGDEVMVKVLEVDRQGKI
                     RLSRKEALQEGGGDLTNLGGGSDGPPEE"
     gene            complement(7640..7909)
                     /gene="rpsO"
                     /locus_tag="VF182_16900"
     CDS             complement(7640..7909)
                     /gene="rpsO"
                     /locus_tag="VF182_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX6438818.1"
                     /translation="MGVAPQQKQGVIEQFRVHQSDTGSPEVQVALLSQRIDYLTQHFK
                     IHAKDHHSRRGLLKLVGQRRRLLDYLKKSDFERYQVLIQRLGIRK"
     gene            8112..8723
                     /locus_tag="VF182_16905"
     CDS             8112..8723
                     /locus_tag="VF182_16905"
                     /inference="COORDINATES: protein motif:HMM:NF019889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HEX6438819.1"
                     /translation="MRISIATLLLQVLFLIPVPRSQAADFFRWIDHNGVVHFTDNIHS
                     IPEARRATAVRIKGQQPPRPAEPSKVSPATVAKASIPFEKHGQVVVVEVMLNKSVPAK
                     LVVDTGATYTMISAATAKELSIDPERTPRTMPFQTANGVIQAPLANLESVIIGGMEVK
                     NLTTAIHDAVPSAQVAGLLGLNFLSNFRLDIDTEKGILHLEKK"
     gene            complement(8903..>9130)
                     /locus_tag="VF182_16910"
     CDS             complement(8903..>9130)
                     /locus_tag="VF182_16910"
                     /inference="COORDINATES: protein motif:HMM:NF036720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAVED domain-containing protein"
                     /protein_id="HEX6438820.1"
                     /translation="GEAVDIIQIIGKALRAARDTFQPRGKMHLFLAVPAGLAMMLGQT
                     LNTFGPIQTYEHIATDAVGMYHPSVFLSPSG"
BASE COUNT         2115 a         2591 c         2281 g         2143 t
ORIGIN      
        1 ctctcggctc cgcgattgtc gacaaatcca attgctcgag ctcgtcttga ctcttaggcc
       61 gttcggcggg ccggtgataa accggagcct ctttggttaa agcgctaatc ggaatgcgcg
      121 ctacttgatc accgttgaac acagctcgga actcgttgtc agccgtgacc cgaccaacca
      181 ccaccgcctc aagatcccat ttgtcgaata ttcgcttgat ctccgcttca gtgccggctt
      241 tggccactag aagcatccgt tcctgagact cggagagtaa aatctcgtaa ggcgtcattc
      301 cctgttcgcg caatggcact gccgataaat tgagctcaat gccggcgccg ccgcgagcag
      361 ccatctcggt cgatgaactg gtcaatccgg cggcgcccat atcttgaata ccgatcaggt
      421 gatcatgctc catcaattcc aaacaggctt ccagaagcag cttctcagtg aatggatccc
      481 cgacttgaac ggttgggcgg cgctgctctt tttcttcgga aaaggattca gaggccatgg
      541 tcgcgccgtg aatgccgtcg cggccggtct tggagccgac atagattacc gggttgccta
      601 cgcccttcgc cagcccggtg tggatgcgct tcttcttggt gattcctaac gtgaacgcat
      661 tgaccacaat gttggcgtca tagcattcgt cgaagtaaac ctcgccacca acggtgggga
      721 caccgatgca gttgccgtaa ccgccgatgc ccgcaaccac gcctgagact agatagggcg
      781 tgcgcggatg atcgatgcgg ccaaaccgca acgaattcaa cgacgcgatc ggccgtgcgc
      841 ccatggtaaa aatgtcccgc aaaataccgc ccaccccggt ggcagctcct tgatagggtt
      901 cgataaaaga tggatgattg tggctttcca tcttgaatgc cacggccaag ccatcgccaa
      961 tgtcgaccac gccggcgttt tcaccgggac cctgcagaac atacggccct tcggtgggca
     1021 aactcttcaa atatcggcgc gagctcttgt agctacaatg ctcggaccac ataaccgaga
     1081 acacgccgag ctcggtataa ttgggttccc gtccaagaat ctggatgatt ttccgatact
     1141 catcgggcgt gagtccatgg ctcgcagcca gctcagctgt aacgggtggc ttcttagaag
     1201 cgttctggtc aagcatgcgc agcgctcttt aaatcgcagg ctgccgcaat cgaggcaaaa
     1261 atcttcagac cgtcatcgct gccaaggagc ttgttgcagg caagttccgg atggggcatc
     1321 aagccaaaaa cattgcgccg ttcattgcag atacccgcga tgtcatctat tgagccgttg
     1381 ggattagcgg gtggcgaagt ttttcctttg gcattgacgt accgtagaag aacctgcctc
     1441 tgccgcttta atcgctccaa tgttgtttca tcagcgtgat aacggccttc gccgtgcttg
     1501 accggaatct tcagcagctg cccttttttc agctgagagg taaagggcgt gtcattctct
     1561 tccacgcgca gccaagtttg ttgacagacg aacttcaggc cggcatttcg cactagcgcg
     1621 ccgggaagca atcccgcttc acagagaatt tgaaaaccgt tgcaaatgcc tagcaccagg
     1681 ccgccttctt gagcgaattt ctcgacgccg cgcataatcg gcgaaaaacg cgccatcgcg
     1741 ccgcagcgga gataatcgcc ataagagaag ccccccggga gaaccagaca atcgaaatgt
     1801 ccgactaact catccttgtg ccagagaaac ttgaccttct ggtccatctc tcgtttaagg
     1861 ctgtcgaaaa catcctgatc gtcgcaggaa cccggaaaaa caatcacacc ccaacgcact
     1921 ctgtcaccct cctctatttt taccttcgag gaccagaaac atcccgatga tcgaatcaac
     1981 ctggaaataa atacgtgcca acgcggctgt cagccttcgg agatttcgta atggaaattt
     2041 tctatgaccg gatttgccag cagcttgtcg cacatctcac ggatacgatc ttccgcggct
     2101 tctcgcgaac cagcctgtaa atcgagctcg acaaacttgc caactctaac atcctgaact
     2161 tctttgtagc ccaaggaatg cagcgattgc tcgacagcct taccttgcgg atcgagcaca
     2221 ccctttttta gtgacacgaa tattttgacc cgcattggca ccttcagtcg gtcttctccg
     2281 gcgatctatt taacggaata ccctgcgaaa gatgaagtct acattggcaa gataatgctt
     2341 taaaccccac accgcttcca cttcactggc cgagagataa ctcatgatct cctggtcctt
     2401 tagcaagtca ccttttagct caccaccatc tctggcagtt ttcaaggcat ggcgttgaac
     2461 catgcgataa gcggcatctc gagatacgcc cttctcgacc aaagcgagaa gcactcgttc
     2521 cgaaaacacc gctccgccgc ttttttccag gttgcgtttc atgttctcag ggtagacaca
     2581 taacgctccg agcacatagg tcattcgccg caacatgaag tcgagcgcga tggttgcatc
     2641 cggcgcaatc actcgttcca ctgaagaatg gctgatgtct cgctcatgcc agagcgccac
     2701 attctccaac gccgccaatg cgtaagaacg catcaggcgc gccagtcccg acacgttctc
     2761 cgataagatc ggattacgct tgtgcggcat cgccgatgaa cccttctgcc ctggcgtaaa
     2821 cggctcttcg gcctcctgca cctccgtccg ctgcaggtga cggatctcaa cagcaaattt
     2881 ttccagcgaa ctgccgatca cggccaacgt agcaaaaaaa taagcatgcc ggtcacgctg
     2941 aatgatttga ttggagatca aggcgggttt aagtccgctc ttcttacaca cataggcttc
     3001 cactttgggg gagatctggg cgaaggtgcc gacggcgcct gaaatctggc cgacactgat
     3061 atcttcgacg gctttcctca gccgaacaag gtttcgctct gtctcttgat accaaagcgc
     3121 aagctttaaa ccgaacgtga tcggctcggc atgtacacca tgagtgcggc cgatcataac
     3181 cgtcgacttg tatctgttgg cttggcggcg caggactttc atcaagctct gcacgtcttc
     3241 aatcaaaatt gccgcggctt ctttgagctg aatcgccaga gccgtatcca tcacatcgga
     3301 tgaggtcata ccgagatgaa gaaatcgcgc atcatcgcca atggattccg ctacggagct
     3361 tagaaatgca atgacttcgt gtttgacctc acgctcgatt tcgcgaatgc gctcgacatt
     3421 gaagcgcgca tttttcctga tccggctgat agctggtttg gggactttgc ccatctgtgc
     3481 cagagcctca gcggccagaa tttcgatgtc cagccatttt tgaaagctat tttcctccga
     3541 ccagatgcga cccatttccg gtcgtgtata acgtgggatc atattcgcgg taaccccgta
     3601 gcttaactat tgcgtaatgg tgagtaggat gaagcgcgct cggggttcaa ttttttcgta
     3661 gggcgtcggt atggccaaag cctccctcct gccgctcgga ttgaggcaat tcggggactt
     3721 cttgccacat tgcccgcaca acccgttgga tgatcatttg cgcgattcgc tgtcccggcc
     3781 gaatgatgat tgcatcggaa cccaaattga tggcaatgag ctgaatttca ccgcgatagt
     3841 ctgcgtcgat ggttccgggg ctattgatca gggtcagccc ttctttcaag gcaagtccgc
     3901 tgcgcgggcg aatttgcgcc tcgaacccga agggcagcgc gattgcaata ccggtcggaa
     3961 ttagccttcg ctctagagtt cgcagttgga gatcatgaga gaggtcggcg catagatcca
     4021 tgccggcggc gccttcggtc atataggatg ggagtgaagc cggcgcgcca ctggcacgga
     4081 cccgcttaat ggctatctga atctcttcca cggcggccga actccgagtg gtcggcagga
     4141 ctactggccg agaccaaagt ttgcgatttt gaaatcatcc ttgaaatcgc ccagcaggga
     4201 aactgccatc gcctcgggcc gaaataaggc gcgcgccagt tcaataattt gatcacgagt
     4261 gacggaacga atccgctggc aaatctcatc agtggtaatg gatttgccga agtaaatttc
     4321 attacgagcg atatgactca tccacgagtc ggtggattcc aagcccaaca tgatatttcc
     4381 caccaattgt ccccgggtgc ggcgaacctc gtcttcgtta atgtctccgg ccgccagaca
     4441 ccttaactgc ttcaaaacga ggtcaacgac ttcctcagcc gcttctaagc tggtccccgc
     4501 ataaatgccg agataacccg tatcgctgta agtcgacatg aaagaataaa cggaatatgc
     4561 tttgccgcgc ttctcgcgaa tttcctgaaa caaccgagaa ctcatgccac ccccgaggat
     4621 ggtgttcagt atatagcccg catgtcgctg cgggtgcgtc tggtgaatac ccggcatacc
     4681 taaacataga tggacctgtt ccaaaggctt aggatgggga aagatgccgc ttcgggtttg
     4741 cggcaggagc tcggcattga ctcgacttgg aaccgctgaa acagactttt ttgatccgcc
     4801 cagacgcgta ttcaactctg tcaccagttt gtcatgttgg aagtggccgg cggcggcgac
     4861 aacaactctg ctcggatgat atcgttcgtg aaagaagttg acgaagtctt cccggcgaaa
     4921 cttcgagaca gttccaacat gaccggcgat cggtctgccc aatggatgat gcgggaaaaa
     4981 atctaaacta aacaggtcgt gcacgtagtc gtccggagta tcttcagcct gggaaatctc
     5041 ttgaagaata acggatcgtt cacgctcgat ctcttcggcg tcgaaatttg aattgagaaa
     5101 aatatcggtc agcagatcgc tggccagcgg tagatcttca tcaagcacct tggcgtagta
     5161 gcaggtgtgt tccttggacg tgaaggcatt gagcaggccg cccacggcat ccatctcttc
     5221 ggcgatctgc gccgctgtgc gtgtggtcgt ccccttgaaa agcagatgtt cgataaaatg
     5281 ggatatgcca ttctcgtccg gcaactcatg acgggaacca ttctcaaccc agatccctaa
     5341 actcactgaa cggtgatcgg tcatctcttg cgagacaacc cggatgccat tatccagaac
     5401 gcttttagca aacataggat agatataggg ccagcgcgtt attcttcagg aggtccatct
     5461 gagccgccgc cgaggttcgt taggtcgccg cccccttctt gaagagcctc cttacgactg
     5521 agcctgattt taccctggcg atccacttca agaactttga ccataacctc atcaccttct
     5581 ttaagcacgt ccgcaacacg acgcacacgc tcaggagcga gctgcgagat atgcaccaag
     5641 ccatcggtgc ccgggaaaat ctcgacaaag gcaccgaaat cgacgatctt ccgcaccgtc
     5701 cctttgtaga ttttgccaac ctccgcttcg gccgcaatac gctggaccat ttgtatggcc
     5761 ttttgtgcgg cggcttcatc actcgatgcg atggttacgg tgccatcatc ttcaacatcc
     5821 atttttacgc cggtggcttc gatgatcccg cggataactt ttccgcctgg accgatgatt
     5881 tcacggatct tctccggttt aactctgata gtgatgatcc gcggcgcatg gcctgaaacg
     5941 ttggtgcgag gcgtcgataa agccttaccc atctcaccaa gtatgtgcat tcgaccttct
     6001 ttggcctgat agagagcctg gcccattatg ttgcgatcga caccgctgat tttgatgtcc
     6061 atctgtagcg aagtcactcc ctcagcggtt cctgcaactt tgaaatccat atcgccgagg
     6121 tgatcctcat cgccgagaat gtccgagaga atccgaacat gctcgccttc tttgatcaac
     6181 cccatggcga tcccggcaac tggcgcggaa accggcacgc cggcgtccat cagggaaagc
     6241 gatcctccgc atacggacgc catcgaggtc gagccgtttg actccagtat ttccgaaacg
     6301 attcgcaacg tataagggaa gctctcctcg gccggtaaaa ctggcaacaa agcacgttcg
     6361 gcaaggttgc cgtgaccaat ttcgcgccgg cccggaccgc gcaagaactt gacttcgccg
     6421 acgctgaacg gcgggaaatt gtaatgcaac ataaacttct tgaagtgctc gccggtcaac
     6481 gcgtccactc tctgctcgtc ggaagcggtg ccgagggtcg tcactaccaa tgcctgcgtc
     6541 tcgccgcgtg tgaacagggc ggagccgtgc gtccgcggca atatatcgac ctcgcaacta
     6601 atcggccgga tatcttttaa gccacggcca tcaatgcgtc gctctttctc gatcaccaag
     6661 tttcgaaagc aattcctttt tagctcttcg aaggcgccct tgatgtcctt ggccttatcc
     6721 ggatgctcgg ccaatgctag cggcaccacc tcacttttca cttctgcgat gcgcttgtag
     6781 cgctccaact tttcggggac ctcgagcgct tgcctgagtt ttgacaaagc caaaccttca
     6841 acccgtcccg ccgcggcttg atcgagctcc gccagcggca cgtgacgttt gggtttgccg
     6901 atcgcgcgcc gaatttcctc ctgcatttcc aacaagggtt gaatggcctc atgcccggca
     6961 aacaaagctt cgagaatttc atcctcgggg attatctggg cgccgccctc aaccatcaca
     7021 attgccgccc gacttcccga gagaaagata tccatatcgc tgtctgaaag ctggctctgc
     7081 gttggattca tgactagttg ccgatcgatc ctgccgattc gcacacctgc caaaggaccg
     7141 ttgaaaggaa tgtccgaaac ttctagagcg gctgaggctc cgagcatcgc gacgacgtca
     7201 ggatcgtttt cccgatcggc agaaaggacg gtggcaatga tctgggtctc gcaacggagg
     7261 ccttccgcaa acagcggacg aatagagcga tcgatcaatc gacacgtaag aatctctttt
     7321 tccgagggcc gcccctcacg cttgaaaaac ccgccgggaa ttttgccggc ggcgaacgtt
     7381 ttctcctggt aatctaccgt caggggaaaa aaatccgtgt ccaacttaaa atcttttgcg
     7441 gctacggcgg tcgccaaaac cacagtttcg ccataacgaa ccaaagccgc accatcggcc
     7501 tgcttcgcca gacgcccaac ctcgatggat aaaggtcgac cgtgaaaatc caactcaatt
     7561 ttcttaatca tagacgctcc tctaaattga gaggtgcttg acggagggta aggatggagg
     7621 gcaatcggtt aggaaaccgt tacttcctta ttccgagtcg ttggatcaga acctgatatc
     7681 gttcgaaatc tgatttcttc agataatcaa ggagccttct cctttggcct accagcttca
     7741 ataggcctcg acgggaatga tgatctttag catgaatttt gaaatgctgg gtaagatagt
     7801 cgatgcgctg gctcaagagc gcgacttgca cttcaggcga gccggtatcg ctctggtgga
     7861 ccctgaactg ctcgataacc ccctgcttct gctgtggagc aactcccatg ttctccgctt
     7921 tttctctctt tcttttgtta tcgatgtttt ctaacatagt gacttggcat tgtaaagctg
     7981 cagctctgcc gagctgcctt caggcagagc aattggcccg gtttgatatc gtttaggcaa
     8041 gcggccggtt agattaatca aaaaactccg aaggacattt ttttggcatg gcaatatgct
     8101 aaagtcaggc cgtgagaatt tcgatcgcca cactgctgct gcaggttctg ttcctcattc
     8161 cagtgccgcg ctcgcaggcg gcggacttct tccgctggat cgaccacaac ggcgtcgtcc
     8221 acttcaccga taacatccac agtattccgg aagcgcgccg tgctacggca gttcgaataa
     8281 aagggcaaca accgcccaga cccgcagagc cttccaaggt ttcgccggca accgtggcca
     8341 aagcatccat tccatttgaa aaacacggac aggtggtcgt cgtagaggtg atgctcaaca
     8401 agagtgtgcc agccaaactg gtggtcgata ccggcgccac ctatacgatg atttcggcgg
     8461 cgactgctaa agagttaagt atcgatccgg agcgaactcc gcgcacgatg ccctttcaaa
     8521 ccgcgaacgg cgtgattcaa gcacctcttg ccaatttgga atccgttatc attggcggaa
     8581 tggaagtgaa aaatcttacc acggcgattc acgatgcggt gcccagcgcc caggtcgctg
     8641 gtcttctcgg tctcaacttc ctgagcaact ttcgtttgga tatagatacc gagaagggca
     8701 tactccattt ggaaaagaaa taagcgatgc ctaagtcgac gaacctaact ataagctctt
     8761 tatccaatcc tactccggac caacgctcga ccggcagtgc ccggacatcc tagttagtgc
     8821 aatctgctca actacttacg agctttttaa acaacctaaa ctaacgcaca gcatcgcagg
     8881 tggtcacact tgtccgtaag cttcagccgg atgggctaag gaaaaccgaa gggtgataca
     8941 taccaaccgc atccgtagct atgtgttcat aagtctgaat cgggccaaaa gtgtttaaag
     9001 tttggcccaa catcatcgca agtccagctg gaacagcgag gaagaggtgc attttgccgc
     9061 gcggctggaa agtgtctctt gctgcacgca gcgctttgcc gatgatctgt atgatgtcca
     9121 ccgcctctcc
//
LOCUS       DASUVS010000314         7696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1878522, whole genome shotgun sequence.
ACCESSION   DASUVS010000314 DASUVS010000000
VERSION     DASUVS010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878522"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="VF182_16915"
     CDS             <1..131
                     /locus_tag="VF182_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX6438821.1"
                     /translation="MAKTIQLIIEYDPKPPFDSGSIAKAEPSIVELAKHAAQDAFK"
     gene            524..1699
                     /locus_tag="VF182_16920"
     CDS             524..1699
                     /locus_tag="VF182_16920"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX6438822.1"
                     /translation="MALSREENEILTRVGKGTPGGEMLRRYWHPVGFSNELKTRPVKR
                     RLLGEDLVLFRDDQGRAGLLGLYCTHRGTSLEFGHVEDGGLRCCYHGWLYDVSGKILE
                     TPGEPADSTFHQRVRHPAYKLQELAGIVFAYLGPDPAPLLPRYDVLVRDDGMRSLAAR
                     IVHCNFLQMVENSVDQHHFKWLHRTPKTRFWKDEKLTSDPTEFGIRDTFTRRVGDEYF
                     RTISLFLMPNMNKVGYHLPENHPAAFAATHEGYEALRWRVPIDDTNTMHFTLYFSPII
                     DGTAAKIPSDQSDKGLLDSVHGKYRWDEETGWIARGDQDRCAQESQGAIFDRTQEHLG
                     VSDEGVILLRKIFKQSIEAVQRGEDPVGIIRDPAKNEILRWTPAEFRLDPESDRAGG"
     gene            1845..2924
                     /locus_tag="VF182_16925"
     CDS             1845..2924
                     /locus_tag="VF182_16925"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6438823.1"
                     /translation="MYSRRYLGCLSFSIFFLFTAAPCFTAESLDPLIAAAKKEDEITF
                     VAGAQTFGGRKGLGDLEAAFNKRFGLKTRINFAAGPDMNARAARHITEIKSGRKASSD
                     IFLGSQSHQALMHVENALEKVNYAGLFPWVTKEMEIYPNEAVLVYTSPNGIVYNANLI
                     AKDKAPKNYLDLVDPRLSPSWAGKLAIPPYVAWLAELSLNWGQEKVKDFARKLVAISG
                     GRLRYSEEERIVSGEFPVMANLGDALGAMWTWQAKGAPIIAVPGTSPINTDYFQLSVP
                     KNVAHPNLAKLFVAFMATKEAQAIIQKHESRSSHLVEGTMMQKYLKENRIQVQEPKES
                     IAYYLKGETDDGLQFKAELAKILKQ"
     gene            3103..4236
                     /locus_tag="VF182_16930"
     CDS             3103..4236
                     /locus_tag="VF182_16930"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX6438824.1"
                     /translation="MLSKEENEILTRVGRSTPGGEMLRRYWWPVGFSELVTTKGQPTK
                     VRLLGEDLVLFRDGAGNLGLLALHCSHRGTSLEFGRVEDAGIRCCYHGWLYDTVGKCL
                     EQPAEPAESSFRNRVQHPAYRAQEIGGFIFAYIGPEPAPLLPNYDLFLRQDGERHVGA
                     GHDYCNWLQRAENSVDQTHLVALHAPEYPHMALKRPVIGWERKPYGAKISMHVPGVTK
                     PKFSHWIFPSHTRHTTARIGRIPDHALRFRVPVDDTNTKTFWLRFYPYGESDEGKPLT
                     LKTVGYENDQPGIYQRVDDGWWGIASSDQDRVAQESQGPIYDRTKEHLGAADEGVILL
                     RAMIKESIDAAVDGKDPIWILRDAAENQNIGFDASMQEIGPLV"
     gene            4673..5800
                     /locus_tag="VF182_16935"
     CDS             4673..5800
                     /locus_tag="VF182_16935"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX6438825.1"
                     /translation="MLTKEENELMTRVGPGTPAGEMLRRYWWPVAFTEVVRPETRPTK
                     VKLLGQEFVLFRDGSGKLGLLELHCSHRGTSLEFGRVEKNGIRCCYHGWLYDRAGKCL
                     EQPAEPEGSTFKDRIQHPAFHAQDAGGLVFAYVGPEPAPLLPSYDLLVREDGCRVVGG
                     GEEFCNWLQRAENSSDGAHSIALHAAGYPNMAMKRPTMKWEPTALGIKETTWVEGVTK
                     PRISHFVFPSHVRHSAARVGEQPRQVIRFRVPADDTTTTTFWIDFYPHKDGQPTRPEP
                     LKTRGFKKDQPGVYERVEDGWWNLPNREQDRVAQETQGAIADRTKEHLATSDQGILML
                     RKMIREAMEAVRQDKDPAGVFRSAHDPIVFDSSRDEVEALT"
     gene            5966..7024
                     /locus_tag="VF182_16940"
     CDS             5966..7024
                     /locus_tag="VF182_16940"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6438826.1"
                     /translation="MLRTIALLIACLSFALRTYPVEAQTLEQLIAGAKKEGEVTLVAS
                     ASTFGGKKGFAELEAGFNRRFGLKHKVNLAPGPSFPQVAARVLTELKSGAKSSTDLYL
                     GSDGTMSDMSREKALHKVNWSATFPWVNKEMEIFPQETLLVYASFHGVIYNSNAIPKD
                     KAPKSYEDLVDPALSPTWAGKIAIPSYIHWLVRLSSIWGKEKVLAYARKLAPLAAGRL
                     RQGEEERIVSGEFPIMASTGGALEAMWKWQAKGAALVGMPGSTPPTSSYYQLAVPRNS
                     AHPDSAKLFVAFMTTREAQAVIEKHDWRSSYLVDGTLMAKYVRGNKIKLQDPRELTEV
                     FMKDDTPFEDELTKILLK"
     gene            7059..>7696
                     /locus_tag="VF182_16945"
     CDS             7059..>7696
                     /locus_tag="VF182_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6438827.1"
                     /translation="MSVIDNTQILHPRPSSVNWSQLFILAVAGLAAVAILGLLITIVW
                     MSFRTGVPGQDSPYTLSNYTSLLADSYNYRVMVTTLIFSGVTIAVAIPLGFLFAWFIE
                     RTDLRYKALAMSLLSIGILFPTFLKAMGWVFLCHPRIGVINIFLMQLLNLKSAPLNVA
                     TLGGIGFVQGLTLTPLAYVMISAALRSMNPALVEASNVHGVSKLKTLLWVELP"
BASE COUNT         1763 a         2048 c         2174 g         1711 t
ORIGIN      
        1 agatggcgaa gaccattcaa ttgattatcg aatacgatcc aaaaccaccc ttcgattccg
       61 gatccatcgc aaaagcggag ccttcaattg tggaattggc gaagcatgca gcgcaggatg
      121 cgttcaagtg aacatttatc gcatctttgt cgcatacgaa aataaccata tcattgcggc
      181 cgatgagttg gtccagaatg agcggtcaca gaatgagggg tgagatcctt gccgtgccac
      241 attgagggtt acgtttgctc ggtatgtcgg gcgaagctgg agccaaatca gctccgatga
      301 gataggcaag cggctgggac gccatcgatt atgcttagcc ggtcgtatcg agattacgaa
      361 atgaaacggg atctgcagag agaaaaaact cttcttaagc cgtagagtcc aggctcatgc
      421 ctgaccggtc ctgcctcccg cgggtgctta ttgacacccc cggacgtgct cggctatttc
      481 tggcgcagtc acagagtttc ccggttgaag cgaggaaaac gtcatggctt tgagtcgtga
      541 agagaacgaa atcctaaccc gtgtcggcaa gggcacgccc ggtggtgaga tgttacgacg
      601 ctactggcat ccggtcggtt tttctaatga attgaaaact cgtccggtga aaaggcggtt
      661 gctcggggaa gacctggtgc tattccgcga tgaccaaggg cgcgccggtc tgcttggatt
      721 gtactgcacg catcgtggca cctcactgga gttcggccat gtggaagacg gcggcctgcg
      781 ctgttgttat cacggttggc tttatgacgt ttccgggaaa atcttagaaa cacccggcga
      841 gcccgcggac agcacgtttc accagcgggt tcgtcacccg gcctacaaac tgcaggagct
      901 tgcgggaatc gttttcgcct atctcggacc cgatcccgcg ccgctgttgc cgcgctacga
      961 tgttcttgtg cgcgacgatg gcatgcgctc gctggccgct agaatcgtcc attgcaattt
     1021 cttgcagatg gtcgagaaca gcgtggatca gcatcacttc aaatggctgc accgcacgcc
     1081 taaaacgcgc ttctggaaag atgaaaaatt gacttcggat cccaccgagt tcggtatccg
     1141 tgataccttc acccgccgcg tcggcgatga gtatttccgc accatcagtt tgttcttgat
     1201 gccgaatatg aacaaggtcg gctaccatct gccggagaat cacccggcgg cttttgccgc
     1261 gactcacgaa ggttatgaag cgctgcgctg gcgcgtgccc atcgacgaca ccaataccat
     1321 gcatttcaca ctttatttct cgcccatcat cgatggcaca gcggcgaaga taccgtctga
     1381 tcagtcggac aaagggcttc tcgacagcgt ccatggcaag taccgctggg acgaggagac
     1441 cggctggatc gcgcgcggcg accaggaccg ctgcgcccag gagagccaag gcgcgatctt
     1501 tgatcgtaca caggagcacc ttggtgtgtc ggatgaaggc gtcatcttgc tgcgcaaaat
     1561 attcaagcag tccatcgagg cggtgcaacg cggcgaagac ccggttggaa ttattcgcga
     1621 tccggccaaa aatgaaatcc tccgctggac tccggcagag tttagactgg atccggaaag
     1681 tgatcgcgcc ggtggataaa caccaaggaa aatccgaaat ccgaatttcg aaatccgaaa
     1741 caaacgtgaa gatactttat agcaatatac gaaacgggct cgtttggaat tttcctggtt
     1801 ctcgtcattc gagtttgttt cgaacttcgg atttcggatt tcgaatgtat agtcggcgct
     1861 atctgggatg tctttcgttc tcaatttttt tcctcttcac cgctgcgccc tgctttactg
     1921 ctgagtcctt ggacccattg atcgccgcgg ccaagaaaga agatgaaatt accttcgttg
     1981 ccggcgctca gactttcggt gggagaaaag gtctgggtga tctcgaggct gcattcaata
     2041 agcgattcgg cttgaaaaca cgcatcaact ttgcggccgg ccccgacatg aacgcgcggg
     2101 cagcgcgcca catcacggaa atcaagtcgg gacgcaaagc ctcgtcggat atttttcttg
     2161 gctcgcagtc gcatcaggcg ctgatgcacg tggaaaacgc cctcgagaaa gtgaattatg
     2221 cggggctctt tccttgggtg accaaagaaa tggaaatcta tcccaacgaa gccgtgctcg
     2281 tttacacctc gcctaatggc atcgtttaca acgcgaactt gattgccaaa gacaaggcgc
     2341 cgaagaacta tctggacctc gtcgatccgc ggttgagtcc ttcgtgggcg gggaaactgg
     2401 cgatcccccc gtacgttgca tggttggccg aactatcgct caactggggc caggaaaaag
     2461 ttaaggactt cgcacgcaaa ctcgtggcga tcagcggcgg ccggctacgc tacagcgaag
     2521 aggagcggat cgtcagcggc gagtttccgg tcatggcgaa tctcggcgat gcgctgggcg
     2581 ccatgtggac ctggcaagct aaaggcgcgc cgatcatcgc ggttcccggg acgtcgccga
     2641 tcaataccga ttattttcag cttagcgtcc cgaagaatgt cgcccatcca aatttagcca
     2701 agcttttcgt cgcgttcatg gcgaccaaag aggcgcaagc gatcatccag aaacacgagt
     2761 cgcgtagctc acacctcgtg gagggaacca tgatgcagaa atacctaaag gagaatcgca
     2821 tccaggtgca ggagccaaag gaatcgattg cttactacct caagggtgaa accgacgacg
     2881 ggttacagtt caaggctgag ttggcaaaga ttttaaagca atagcaggct gctgagtaat
     2941 gctcatataa tttcgaaaag atgaattatg aaagctgaat tccgcttgcg gatttccgct
     3001 ttcatccttg taattcgcgc gcctcacctc tgaacatttt tgagcaggtg cgattttcaa
     3061 gccagaaagt tgagactaga aaccagaaac tctgaggggc aaatgttgtc caaggaagaa
     3121 aatgagattt tgacacgcgt ggggcggagt acgccgggcg gcgagatgct gcggcgctac
     3181 tggtggcctg tagggttttc agagttggtt acgacaaaag gtcaaccgac gaaagtgcgg
     3241 ttgcttggcg aggacctggt gcttttccgc gacggcgctg gaaatctcgg cttactggcg
     3301 ctccactgct cgcaccgcgg cacgtctttg gagttcggcc gcgtcgagga cgccggaatt
     3361 cgctgctgct atcacggctg gctctatgac accgttggaa aatgcctgga acagcccgcc
     3421 gaaccggctg agagcagctt tagaaaccgc gtgcagcatc cagcttatcg ggcacaggag
     3481 attggcggat ttatatttgc ctacataggt cccgagccgg cgccattatt gcccaattac
     3541 gatctcttcc tgcgccaaga tggcgagcgc cacgtcggcg ccggccatga ttattgcaac
     3601 tggctccagc gcgctgaaaa ctctgtggac cagacgcatc tggttgcctt gcatgcgccc
     3661 gagtatccgc atatggcgct taagcggccg gtcatcggtt gggagcgcaa accttacggg
     3721 gcgaaaattt ccatgcacgt tccgggtgta accaagccga aattttccca ttggattttt
     3781 ccttcgcata cgcggcatac caccgcgcgg atcgggcgta ttccggatca tgcgctgcgc
     3841 tttcgcgttc cggtggacga taccaatacg aaaactttct ggttgcgctt ttatccgtat
     3901 ggcgagagcg acgagggcaa gccactgact ctgaaaaccg taggttatga aaacgaccag
     3961 ccgggcattt accagcgggt cgatgacggc tggtggggca tcgcttcgag cgatcaagat
     4021 cgggtggcgc aggagagcca gggacccatc tatgaccgga ccaaagagca tctgggggca
     4081 gcggatgaag gtgtcattct gctgcgcgcg atgatcaaag aatccatcga tgctgcggtt
     4141 gacggcaaag acccgatatg gatcctgaga gatgcggccg agaatcaaaa catcggcttt
     4201 gacgcgagca tgcaggagat cgggccgctg gtttgataat aaccggttca aaacgttcaa
     4261 aatgttcaag tcgttcaaaa cgtttgcgtt agaaattatc cggaagaaat gggttatttc
     4321 cgcaaacgcg ggcatgacgt aacgcaaggt ttattttttt agcgaagcgg agaatcaagg
     4381 atattttcaa aggaagaaaa tgattacttc actcgcgttg ggcgggtact gtggctaatg
     4441 agatgctgcg ccgttactag catcccattg gctttgccgc cgaggtaaac aaacggccca
     4501 ccgcatgaga aaggatgaat tatgaaagct aaaagaaagt tccgttttca tccttccgct
     4561 ttcgtccttg tttcttcgcg cgcctgtacc tgaacatttt tgagcagcct atcgggtaaa
     4621 actcagcatc aaaactcgaa ccaaagacta gaaattccgg agggctaatc aagtgctgac
     4681 caaagaagaa aatgaactca tgacccgcgt tgggccgggc acgccggccg gcgagatgtt
     4741 gaggcgttac tggtggccgg tcgcgttcac ggaagttgtc cgcccggaaa cgcgacctac
     4801 caaagtgaaa ctgttgggcc aagagtttgt cttgtttcgc gacggcagcg gtaagctcgg
     4861 cctgctcgag cttcattgtt cgcatcgcgg tacttcgctt gagttcggca gggtcgaaaa
     4921 gaatggaatc cgctgctgct accatggctg gctgtatgat cgcgccggaa aatgcctgga
     4981 gcagccggcc gagccggaag ggagtacgtt caaggaccgc attcagcatc cggcgtttca
     5041 tgctcaggac gccggtggcc tcgtcttcgc ctatgtcggt ccggagcctg cgccattgct
     5101 gcccagttac gatttgctgg tacgcgaaga tggctgtcgc gtggtcggcg gcggcgagga
     5161 gttttgcaac tggctgcagc gcgcggagaa ttcctccgac ggcgcgcatt ccatcgcgtt
     5221 gcatgcagcc ggttatccca atatggcgat gaagcggccg acgatgaaat gggaacccac
     5281 cgcgctcggc atcaaggaaa ccacctgggt tgaaggagtg acgaagccgc gcatttctca
     5341 ttttgtattc ccgtctcatg tgcgtcactc agctgcgcgc gttggtgagc agccgcgtca
     5401 ggttattcgc ttccgtgtcc cggccgatga tactacgact actacctttt ggatcgattt
     5461 ttatccgcac aaggacggcc agccgacccg tcctgagccg ctaaaaacga gaggctttaa
     5521 aaaagatcaa cccggagttt acgagcgggt cgaagatggc tggtggaatt tgcccaaccg
     5581 cgaacaagac cgggtcgcgc aagaaaccca aggcgcgatc gctgaccgca ccaaggagca
     5641 tctggcgact tccgatcagg ggatcctgat gctccgtaag atgatccgtg aagccatgga
     5701 ggcggtgagg caggacaaag atcccgccgg cgtatttcga agcgcgcacg atccgatagt
     5761 tttcgattcc agtcgggatg aggttgaagc tctgacgtaa aaaatgcgct cgcgaacttt
     5821 gcgaatcgcg tttactccgg cacccccgca aagttgggca tgacagcttc gctgattgag
     5881 aagatgcaca acctaaaatt cacgactctg cgcgaacgcc gcataggcgc tctcgcttgt
     5941 gccgcacttg caggagaaaa ccctcatgct tagaactatt gcattgttaa tcgcgtgttt
     6001 atcatttgcc ttgcgaacat atccggtgga agcgcagacg ctcgagcaac tgatcgccgg
     6061 cgcgaaaaaa gaaggcgagg tgacgcttgt ggcgagcgct tcgaccttcg gcggcaagaa
     6121 aggatttgcc gaattggaag cgggtttcaa taggcgcttc ggacttaagc acaaagtcaa
     6181 cctggctccg gggcccagtt ttccgcaagt cgcggcgcgg gtcctgaccg agctcaagtc
     6241 cggcgcgaaa tcttccactg acttgtatct cggatccgat ggcacgatga gcgatatgtc
     6301 tcgggagaag gctttgcaca aggtcaactg gtcggcgact ttcccgtggg taaataaaga
     6361 gatggagatc tttccccagg agacgctgct ggtttatgct tcctttcacg gcgtcattta
     6421 taactccaac gcgattccaa aagataaagc tcccaagagc tacgaagatc tggttgatcc
     6481 ggcgctcagc ccgacctggg ccggcaaaat tgccatccct tcctacatcc actggttggt
     6541 tcgtttatct tcgatatggg gaaaggaaaa agtgctggcc tacgcgcgca aactggcgcc
     6601 gctggcggcg ggccggctgc gccagggcga agaggaacgc atcgtcagcg gcgagtttcc
     6661 gatcatggcc agcacagggg gcgccttgga agcgatgtgg aagtggcaag ccaaaggagc
     6721 cgcgctggtc ggcatgccgg gctcgactcc gccgacttcg tcttactatc aactggcagt
     6781 gccgcggaac tccgcgcatc cggattccgc caaactgttc gtcgctttca tgaccacccg
     6841 cgaggcgcaa gctgtcatcg agaaacatga ctggcgctcg tcctatctgg tggacggcac
     6901 cctaatggca aagtacgtgc gcggcaacaa gatcaaactt caagatccac gggagctgac
     6961 ggaagtgttc atgaaggacg atacaccctt cgaggatgag ctgaccaaga tactgttaaa
     7021 gtaaatcaga catgcctatc gactaatgcc catcttctat gagcgtcatc gacaacacgc
     7081 aaattctgca tccgcggcca tcctcggtca actggtcgca gctgttcatc ctggcggtgg
     7141 ccgggcttgc cgcggtagca atcctcggac tgctcatcac cattgtttgg atgagctttc
     7201 ggaccggagt gcctggccag gactcgccct atactctgag taattacact tccctgctcg
     7261 cggattctta taactaccgc gtaatggtga cgaccttgat tttttccggc gtcactattg
     7321 ccgttgcgat cccgcttggt tttttgttcg cctggttcat cgagcgcacg gatcttcgct
     7381 acaaagcgct ggcgatgagc ctgctcagca tcggcattct ttttccaacc tttctcaagg
     7441 cgatgggctg ggtttttctt tgtcatccgc gcatcggcgt catcaatatt tttttgatgc
     7501 agctcctgaa tctcaaaagc gcaccgctca acgtcgcgac cttgggcggc attggattcg
     7561 tccagggact gactctgaca ccgctcgcct acgtcatgat ctccgcggcc ctgcgtagca
     7621 tgaatccagc gctggtcgag gcctccaacg tgcacggggt cagcaagcta aaaaccctcc
     7681 tctgggttga gctgcc
//
LOCUS       DASUVS010000315         3431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_567325, whole genome shotgun sequence.
ACCESSION   DASUVS010000315 DASUVS010000000
VERSION     DASUVS010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567325"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="VF182_16950"
     CDS             <1..1142
                     /locus_tag="VF182_16950"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6438828.1"
                     /translation="GAYAVEPKVCSYGDALEQLRGYTGAVIAIMDLSRDGDRAYAVAA
                     EFKSKLSQVRLVMTSPSHNPNEILRAMRSGAEEFLTQPFDWPEVIKSLEAIRDKVDVH
                     SSRTHERGQIVAVSSNKGGVGSTTVATNLAACLVAHKKSVCLVDLVLQFGSVTSFLNL
                     EASYTILDLVKNLKKIDPMFLDGSLVKHASGIRVLAEPFYAEDARRITPADIDEILEV
                     LSQSFDFVVVDTPKEFDESLALVLDKANMILFVTEMDVPSLKSAHRAFELFSRMGIYE
                     KKIRLVLNRFIKSKLMSLESAEKALGAKVFWTLPNNYPTAIAAVNQGLSIQDCDPRSD
                     IARSYSGLSDAVVESFTAVGASRREADDKRVGLLDRWMPVRGLLK"
     gene            1240..2553
                     /locus_tag="VF182_16955"
     CDS             1240..2553
                     /locus_tag="VF182_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX6438829.1"
                     /translation="MRSMQPGNSEAILQDLRLRIHRRLIDTLDLTKLSNLEMERVRVE
                     IRRILEDMVAAEAIPVSRADRDRLVMEVQHEVFGLGPLETLMKDPEISDILVNSSSQI
                     YVERYGKLEKTDVRFRDDGHLMQIIERIVTRVGRRVDESSPMVDARLADGSRVNAIIP
                     PLALDGPVLSIRRFGVERLTVNDLIQFNSIPPQIAEVAAACVKSRLNMLVSGGTGAGK
                     TTLLNCLSNFIPDNERIVTIEDSAELKLQQDHIVRLETRPANIEGQGSVTARDLVRNA
                     LRMRPDRIVIGEVRGGEALDMMQAMNTGHDGSISTVHANSARDALSRLETMMLMAGIN
                     LPERALREQVASALDVIVQLTRLSDGSRKVVEFAEVTGMEGATITTQTIFKFDQRGVE
                     NGKVVGEFVATGVMPSFMDRLERHGFRIPNTHFMPVPLGARKRAL"
     gene            2550..>3431
                     /locus_tag="VF182_16960"
     CDS             2550..>3431
                     /locus_tag="VF182_16960"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6438830.1"
                     /translation="MSLFLIAIFVFLSAMSLVVIIFMLPMAVKDTAQARIKRRLTTIG
                     HMTHASRSELQSLLKAPVYSNNPWLNNLLCNIPLVQKVGLHLERANVDMSPSVFLLCS
                     GGACLITFFMFMIVGKPTWMALLFGIIALVIPSVYLKYVIWRRLRKFLEQMPDGLEMI
                     SQSLQAGQGLTQAMVYVAKEMPDPIGTEFSVFIEEVNLGLPLNDALRKFEQRMSLPEV
                     RLFNTALLVQREIGGSLSELLTKLANIIRDRFRIERLIKSLTAQNRMSAWTVSSIPPF
                     LAVFMFIREPVMMNEMMV"
BASE COUNT          843 a          800 c          922 g          866 t
ORIGIN      
        1 agggggcata cgccgtcgag cctaaagtat gttcttatgg cgatgccttg gagcaactgc
       61 gaggctatac aggcgctgtc attgcgatca tggatctgag ccgcgacggg gacagagcgt
      121 atgccgttgc cgcggagttt aaatcaaagc tttcgcaggt gcgcttagtt atgacatccc
      181 cgtcgcataa tccaaatgaa atcctgcgcg cgatgcggtc gggggcagag gagtttctca
      241 cacagccctt tgactggcca gaggtcatca agtcgctaga ggctattaga gataaagtcg
      301 atgtccattc gtctcgcaca cacgaaagag gtcagatagt tgctgtctcc tcaaataaag
      361 gcggcgttgg atccacgact gtggcgacca acctcgccgc ttgtttggtg gctcataaga
      421 aatcggtttg tttggtcgac ctcgtcttgc agttcggatc ggtgactagt ttcttgaacc
      481 tggaagcttc gtacaccatt ctagatttgg taaagaatct aaaaaaaatc gacccgatgt
      541 ttctcgatgg ttctctagtt aagcacgcat cgggtatccg cgtcctggcg gagccttttt
      601 acgccgaaga tgccagacgc attacaccgg ccgatattga tgaaatttta gaggttttgt
      661 cgcaatcgtt tgatttcgtc gtggtcgata ctccgaagga atttgatgaa agtctcgcgc
      721 tcgttttgga taaagccaac atgattttgt ttgtcaccga aatggacgtt ccgtcattga
      781 aaagcgcgca cagggcattt gaactcttta gcaggatggg aatttatgaa aagaagatcc
      841 gcctggtctt aaaccgattt atcaaaagca agcttatgag tctcgaatcc gcggagaaag
      901 ctttaggagc gaaggtattt tggacgctac ccaacaacta tccgacggcc atcgccgcag
      961 taaatcaggg attatcaata caagactgcg atcctagatc cgatattgct cgaagctatt
     1021 cggggctttc agacgcagtc gtcgaatcgt tcaccgcggt cggcgcaagc cggcgtgaag
     1081 cggatgataa aagggtgggg ctacttgatc gttggatgcc agtgcgaggt ttgctcaagt
     1141 gattaaagga tactgatgaa aaacccatct ctattagata gcaacaaaga taagtgggac
     1201 aacgataaaa gcgccggatt agacaatacg agggtgccaa tgaggtcgat gcagcccggt
     1261 aatagtgaag ccattttgca agaccttcga cttcgtatcc accgcaggct gatcgacact
     1321 ctcgacctaa ccaagctgtc aaatttggaa atggagcggg tgagggtcga aatacgtcgt
     1381 atattagaag atatggttgc ggctgaagcc atccccgtca gtcgcgccga tcgagaccgc
     1441 cttgtgatgg aggtccaaca cgaagtcttc ggtcttgggc ctctagaaac cctaatgaag
     1501 gatccagaga tttccgatat acttgtgaac tcctccagtc aaatctatgt tgagcgctac
     1561 ggcaaactgg agaaaaccga cgtgaggttt cgtgacgatg ggcatctgat gcagatcatc
     1621 gaacgaatcg ttacccgggt tggaagaaga gtagacgaaa gctctccgat ggtcgatgct
     1681 cggcttgccg atggctcgcg cgtcaacgca attattccgc cgctcgcgtt ggacgggccg
     1741 gtgctctcaa ttcgccggtt tggtgttgaa cgtctcactg tcaatgactt gattcagttt
     1801 aattcgattc ctcctcaaat tgctgaagtg gcagcggctt gcgttaaatc acggttgaat
     1861 atgctggtct cgggaggtac gggcgcaggt aaaaccacac tgttaaattg tctttcgaac
     1921 tttattcctg acaatgaacg tatcgttacc atagaagatt cggctgaatt gaagcttcag
     1981 caagatcaca ttgtccgact cgaaacccgc ccggccaata ttgagggcca aggctccgta
     2041 accgcgcgtg atctggtccg caacgcactt cgtatgcgtc ctgatcggat tgttatcggt
     2101 gaggtccgtg gcggcgaagc attggatatg atgcaagcga tgaacacggg tcatgacgga
     2161 tccataagca cggttcacgc gaacagcgcc cgggatgcat tgtctcgttt agagacaatg
     2221 atgttgatgg ccggcattaa tctcccggaa cgcgctctgc gcgagcaggt ggcgtcggcg
     2281 ctcgacgtta ttgtccagct cacgcgtctg agcgacggca gcaggaaagt agttgagttt
     2341 gcggaagtta ccggcatgga aggcgctact atcacgacac agacgatttt taaattcgac
     2401 cagcgtggtg tcgagaacgg caaagtggtt ggagagttcg tcgcgacggg agtcatgcct
     2461 tcctttatgg atcgtctcga acggcatggc tttagaattc caaataccca ttttatgccg
     2521 gttcccctag gcgcaaggaa aagagcacta tgagtttatt cctcattgcg atcttcgtct
     2581 tcttatcagc gatgtcgctg gtcgtgatca ttttcatgtt accgatggcg gtcaaggata
     2641 ccgcgcaagc caggatcaaa cgccgcctga cgaccatcgg tcacatgacg catgcctcaa
     2701 ggtcggagct tcaaagtctt ctaaaagcgc cggtgtatag caataatcct tggctaaaca
     2761 atcttctttg taacattccg ctggtacaga aggtcggttt gcatctggaa agagccaatg
     2821 ttgacatgag tcccagtgtt ttccttttgt gctccggcgg agcatgtctc ataacgttct
     2881 tcatgttcat gattgtcggg aagccaactt ggatggcgct gctatttgga attatcgcgt
     2941 tggtaatccc atcggtgtat ttgaaatacg tgatctggag acgcttacgc aagtttctcg
     3001 aacaaatgcc cgacgggctc gagatgatta gccagagtct tcaagccggt cagggactga
     3061 cacaagcaat ggtttatgtc gctaaggaga tgccggatcc gatcggcacc gaattttcgg
     3121 ttttcattga ggaggtgaac ttgggattgc ctcttaatga tgcactgcga aaattcgaac
     3181 aacgcatgtc tctcccagag gttcggctgt tcaacaccgc gttgctggtt caacgcgaga
     3241 ttggcggcag cctctcggag ctcctcacca agttggcaaa tattattcgc gatcgtttcc
     3301 gcatagaacg attgatcaaa agtttgacag cgcaaaatcg catgagtgcc tggacagtaa
     3361 gctcgatacc gccatttttg gcggtgttca tgtttattcg cgaaccggta atgatgaacg
     3421 aaatgatggt g
//
LOCUS       DASUVS010000316        16237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_567451, whole genome shotgun sequence.
ACCESSION   DASUVS010000316 DASUVS010000000
VERSION     DASUVS010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 16237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16237
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567451"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1035)
                     /locus_tag="VF182_16965"
     CDS             complement(160..1035)
                     /locus_tag="VF182_16965"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX6438831.1"
                     /translation="MEVIKLKCNKSRRQESYILVALVPFLLSYGCAIYSAVSEPAVQN
                     EQSSPVIVEQQPAQPIVSDSPTRVKNENSRRAFSSQKALSKEEISQLQARLKAAGFDP
                     GPVDGILGYKTRSVILRVQAGCSILNELSASNTDLSAPATETEATALIGSAKSLSKVD
                     VQLLQRRLKAAGFYSGPIDGVLGVRTKSALSHCKSGCGVLNDLSGTSDQPVFEQAKET
                     QSPAVSAFAERSASVDTISGNQEIRQVQGRLKAAGFDPGPIDGKLGPQTTAALEKYRS
                     SRKFTRSGSDALPHY"
     gene            1277..2434
                     /gene="hemW"
                     /locus_tag="VF182_16970"
     CDS             1277..2434
                     /gene="hemW"
                     /locus_tag="VF182_16970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX6438832.1"
                     /translation="MKPFSLYIHIPYCIRKCPYCDFNSHVVAQIPERDYTDALIRELD
                     HYASSPDWQGRTVQTIFFGGGTPSTFTPSSFNLVLERTFSSFIIDPNCEITLEANPGS
                     VDYGYFSGYRDAGVNRISIGIQSFQPRLLRFLGRVHTAEEGRKALEVVKKAGFDNFSL
                     DLIFANPGQTLKELEGDLDAALEYRSPHLSAYNLTFEEGTPFHHEYRCGRMTTLTEDE
                     EIAMAELIEDKLGAARLKRYEISNYALADCHSRHNVNYWQLGDYLGLGAGAHSYLQSG
                     MDPMVRTRWSNEKNPGRYMSRINESGIAITDRENIGEDKAASEFLFLGLRMISGVSSE
                     VFRSIFGRAPVEFYPKIETWREAGLMAEENGNLRLTAKGLLLANSIFVELI"
     gene            2595..3779
                     /locus_tag="VF182_16975"
     CDS             2595..3779
                     /locus_tag="VF182_16975"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438833.1"
                     /translation="MSTALSTELNQLRVDHIGSLVRPAMLKDVFARFDRGAASREELI
                     QAQDQAIRQVIASQKAHGLPVVTDGEFRRHSFQESFSECVSGFEVPNNVSLYYEQRDL
                     NLTPLQRAEQNFDEAGPAIITRRAAVRRLTLMRNLPLEEFRYAQSITQVPVKVTVLGP
                     DRIAQRFKWEDSQNVYKDLDDFVDHVVEIERQMIAQLVNAGCKYIQIDAPGYTAYVDK
                     VSLERMRSRGEDPERNLQRSIDADNALIEGFPGVTFGIHICRGNARTTDPITGKLVPQ
                     WHREGSYEAIAERLFNSLKHYRLLLEYDSERAGGFEPLRLVPKEKVVVLGLVTTKSAD
                     LESVDELKRRIEQASKYLPLDQLALSPQCGFGGIDSKVMSEEEMWRKFDRIVETAAQV
                     WS"
     gene            complement(3840..4262)
                     /locus_tag="VF182_16980"
     CDS             complement(3840..4262)
                     /locus_tag="VF182_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX6438834.1"
                     /translation="MALAKKPFAFIRAARVAHLATAGLDNQPHVIPICFVFDGKYFYS
                     PIDEKPKRTSPAKLRRVINIQANPQAALVIDRYSEDWKQLAYVLIGGKAKVLHRGAAH
                     AEAVRRLRRKYPQYRQMAIHSRPMIRFTPTRWTFWGNC"
     gene            complement(4356..5348)
                     /locus_tag="VF182_16985"
     CDS             complement(4356..5348)
                     /locus_tag="VF182_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438835.1"
                     /translation="MEKLFLTVACGNYDRTFALQTGAVQAEGIRINYVPLEAEEIFWR
                     MGHHHEFDVSEMSLSNHVTMISRGNSPFVAIPVFPSRFFRHSCVFINTGSGIKSPADF
                     KGKKIGAPEYSITAAVWIRGFLNDDYGVKTSDMQWFTGGQEETGRKERVKLTLPPQIK
                     LEPIADDKTLNGLLESGEIDALISARSPSSFVKGSPKVKRLFPNYKDVEVDYYKRTKI
                     FPIMHVLVIRREVYEKNTWVARSLYKAFCEAKDVAIKNMHISNTMACTLPWLPWEREQ
                     LREIFGPDWWPYGVESNRHLLEHLIRYMDEQGLLARKLTVDEIFAPNVAGEFKI"
     gene            complement(5380..7128)
                     /locus_tag="VF182_16990"
     CDS             complement(5380..7128)
                     /locus_tag="VF182_16990"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6438836.1"
                     /translation="MDRSIAVGQRLPSAWSRSRVGAFLTQPISWVFIVVGSIIAFLSL
                     YPTVFLFYGSLTDAPLGVPGQFTLQNYVRAYSDPEAYPLLLNSFIFGIGSAGLSVVFA
                     LTLAWITIRTNAPLRRFFELTAIIPNILPPILVSISWVMLLNPSNGLINGLFIQLFGV
                     ERGPFNIYSLAGLIFVEGLILTPLAFLIIAAALKSMDPSLEESAKTLGSGEFGIMRRI
                     TFPLIRPAILAAGTLNFVRAVESFDTPAIIALPARIEVFTTKIWREALGSFPTNHNLA
                     ATYGVGILVIALMFVYLYRRFTSRVESFSTVTGKGFRPHQIDLGKYRYLATAVAFLLL
                     VLLVVLPILVLLLVAILPYYHVPTWQTWQNLTFDHFRYIWATPRVHRAFLNSLFLALV
                     GATICMLLASLASYISVRTKITGRGLLEGLVFIPWAFPGTAMALGLLWAYVDFPIPVY
                     GTIWIILIAYVTRFLPYGLRAVSSTIIQVHKELEEASVVCGAGFLATFRRVLIPMMRP
                     GVMAGWIILVTIFLREFSATLFLYSPGAEPLGPLLYFLYLDGMRGRVAAIGLVISVIS
                     VILIAIAQRYSRWETN"
     gene            complement(7144..8154)
                     /locus_tag="VF182_16995"
     CDS             complement(7144..8154)
                     /locus_tag="VF182_16995"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6438837.1"
                     /translation="MLKNTTLSLAVLASFLFVTCLASAQDAKLIEAAKKEGKVIAYGS
                     LESDTMEAIMNGFKKKTGLEIEYWRASATKVMDRALSEARAGRSVADLIVTNDNPLRL
                     LQKENFFQKYESPTWSDFPKEAIDPVLGPRYRNVIIGVVYNKSGVKTEDAPKSLEDLV
                     NPKYKGKLVMPDPTQHTTTTQWVASLHKVMGKEKAEKYIRDLAAMKPILVESLLPSAE
                     RVMTGETPIAITYVKYAYVFGKRGAPLEYVRLPKMLGDGHYIALAAKAPHPSTGKAFI
                     DYFLDNESMNTMAKLGEFVNRKGVLPPLPGADKIGFVEMEDLSSKEFAEKKKEYQQIF
                     LR"
     gene            8337..9719
                     /locus_tag="VF182_17000"
     CDS             8337..9719
                     /locus_tag="VF182_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HEX6438838.1"
                     /translation="MDKLDLLIKNGEVWTPGGFVEADIAVSGGKIAALGKPPVLPDTA
                     ANVIDAKGKKVIPGLIDTHTHHRDPGFTHKEDITTATMAAAAGGVTFSVGMPNVNPPT
                     TSVEHYRALVAHHSKNAIVDFNHNPSGTVPREVPGLAKEGPIAFKIFMVKDTGRDYPH
                     MPGIGINNNGELFKCFETVGATGLPLMVHPHDQDLMDEIEGRYWKDENRSPQAYAKAY
                     RDHDGIIWDTAMATLFRMQKAIGSKLHILHMSTPEGLAMLRRAKDEGRPCSCEVNPWA
                     MFLGTWENVEKLGPYCLGFWVPPAHVEALWEAINDGTIDVIGTDHAPHTREEKEKGWH
                     DMWSCPGGEPQIQDYLRLFLTAVNQGKLSLDRLVRISSYNAAKLFNVYPRKGVIQPGS
                     DADMVIIDMNKEEVISNETTYTKVGWTPYDGKKIKGAPIRTILRGKTVMEDGKITGKP
                     GDGQLVSPVV"
     gene            complement(9790..11121)
                     /locus_tag="VF182_17005"
     CDS             complement(9790..11121)
                     /locus_tag="VF182_17005"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX6438839.1"
                     /translation="MVRIAPFHGVFYNQKKFRDLARVVSPPYDVISPEEQEKLYRKSP
                     HNFVRLDFHREPDSYAAVAELFANWQAEGIFERDKQPAIYFAVHKFALKNGEIKERQG
                     FYALTQLQDFPGDEIRPHEKTLEAPKQDRLQLMLASHAQLSSIFALYSQPKGSINRIL
                     SEQIEGKEAFLEVKLDDGDECRLWRITDPALIREIQRQMKEQRLVIADGHHRYEATLR
                     YRDQMRSDRSSTGRESFNYIMTHFANINDESVVILPTHRLVRGYEPKAFLQLEEALET
                     YFYIEQYPKTSEGKSSFLRALKSSGKKHRVIGASFKRDPRYLILRLKNKRIMQRLAKD
                     LSAPLRDLEVSTLHHLILEHILGLSLEQQTSGETIRYSQDEEAVLQALEKEDYQAAFL
                     LNATKSADILTIVDSGETMPQKSTYFYPKLISGLVINKIDPSEEVPEPTDP"
     gene            complement(11181..12071)
                     /locus_tag="VF182_17010"
     CDS             complement(11181..12071)
                     /locus_tag="VF182_17010"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX6438840.1"
                     /translation="MKLAGIKPNEIALVKNDALILLGETLVRESALPKGFSMLEMIAK
                     YGSIKDLIESYASKLTPSKLDSKMLKAPIEKPSKIWAAATNYKRGTGGLDDARGRGAA
                     GTATAEQLLERIFLKPPSCICGPEDNIIIPDGAETIFPELELCVVIGKETRNISKERA
                     YEAIFGYTILLDVTARGYGSGKDLSGSRCVRKGFETFAPIGPWITTKDEIADPHQLSM
                     KLWVNGELKQSARTDSMVNDIPTLVSYLSQVTTLFPGDLISTGNPDAPEFQEKLRPGD
                     IVRSEIEGIGAMNLGVAKQN"
     gene            12355..13164
                     /locus_tag="VF182_17015"
     CDS             12355..13164
                     /locus_tag="VF182_17015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX6438841.1"
                     /translation="MSNFLIERGSPGVVIKKNQVQAYFACPEDSATIRRDVQRFLRGI
                     RGMYPEIGEDQLRWRVLKDRNWNYSWRRFFSPQKVGQAFLVAPPWAKLPPLGRRHLIV
                     IEPGMAFGTGTHATTRGCMEFIERVAGSLGPKSFTALDVGTGSGILAIALAKLGASKV
                     WALDNDPVALKVARENIACNGVQQNVFCSAGPLNRLRSFPVVVANLTAETIMSLAEPL
                     GKRVAPAGYLILSGVLRPKSEQLVRYFGAKGFRLVKLKEEKEWATLLLKRK"
     gene            13168..13908
                     /locus_tag="VF182_17020"
     CDS             13168..13908
                     /locus_tag="VF182_17020"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX6438842.1"
                     /translation="MARFFLPTQQIQDRRATLIGDELNHLRRVLRLRTEDRVVLFDDA
                     GCEHEGIIRDLSEDRAEIEILRSYHVDNESPLKITLALGLTKGEKLDWVVEKATELGV
                     HTILPFFSRYTVPKLNETKMAQRSERWQKIAVSAAKQCGRARIPKILALTEFRDLLKT
                     VSGDALPLLFWEKECEQRLSELKETRTSISEVVVTIGPEGGFSAQEAALALDQGFQSV
                     RLGRRILRAETAAVAALAAVQLLWGDLA"
     gene            13994..14650
                     /locus_tag="VF182_17025"
     CDS             13994..14650
                     /locus_tag="VF182_17025"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX6438843.1"
                     /translation="MITHNTGVCSRCNAPLDPTGLRRDSTDEEVRLDSDQEKTDLRDE
                     SSDRMETYIPPSGKADEPPLGSINHQAPDNRLWGGFIRRACAFAVDWAIILLLGLVMF
                     LMSYIGYKVGLSAHGRSVTMENSSGLYFILTWASVALSAAYFVIFHSLDGRTIGKWIF
                     GLRVIGAENGDVTSSQAFLRWLASVGFAPILLGFLWVIWSREKRAWHDIIAQTWVIRQ
                     "
     gene            14678..15634
                     /gene="gshB"
                     /locus_tag="VF182_17030"
     CDS             14678..15634
                     /gene="gshB"
                     /locus_tag="VF182_17030"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEX6438844.1"
                     /translation="MKPLKIGVVMDPIEKIDIDKDTTFVLMLEAQHRGHEIYFMEIDD
                     LFARGGTPCARYRRLHLARAVPHYHLTQIDTRPLEDLDVVLMRKDPPFDMKYFFATHL
                     LSLIDEKKCFVMNNPRGLRDANEKLYALRFPEQIPQTLVSSSIERLKAFMDELGGEMI
                     VKPLDGSGGSGVFYLHQQDRNTNSILEAATDNGRLMIMGQRYLPEIRQGDKRIIVLNG
                     KPLGAVLRVPLESEIRGNIHVGGQCVKTELTARDREICEALASLLIADGLYFVGLDVI
                     GNFLTEVNVTSPTGIQEINALDSVRLESSVIDFVEEQVGRIQ"
     gene            complement(15857..16147)
                     /locus_tag="VF182_17035"
     CDS             complement(15857..16147)
                     /locus_tag="VF182_17035"
                     /inference="COORDINATES: protein motif:HMM:NF015959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SpoVG family protein"
                     /protein_id="HEX6438845.1"
                     /translation="MQITNVKIYRTVQGTLRGYADVTFDDCLSITEFKLLRTATGYRV
                     CMPEVKQQDGKVRRVAYPTDDRMLKAIEDAVIAEYKVTGASIRSRRRVWLPT"
BASE COUNT         3827 a         4177 c         4419 g         3814 t
ORIGIN      
        1 ggtctcaacc cggggaagtg atcgacggtt gcaaacgagc gcagccgttg gactcgtcag
       61 ctattatgga cggccattcg tcgatggcgg atacgaacag agaaccccgt caaaggagct
      121 tctgatggtt gtattgggaa aaagtttttg atcgttcgat caatagtgag gcaacgcgtc
      181 gcttcctgaa cgggtaaact tgcgggaaga gcgatacttc tcgagtgctg ccgtggtttg
      241 aggtcccagt tttccgtcga tgggacctgg atcaaatccg gcagctttca gccgcccttg
      301 cacttggcga atttcctgat tgccagagat ggtatcgacc gaggccgatc gttcggcaaa
      361 ggcggaaacg gctggcgatt gtgtttcttt agcctgttcg aagaccggtt ggtctgaggt
      421 tcctgaaagg tcgttaagca cgccgcatcc cgatttacag tgggaaagcg ccgacttggt
      481 tctaacgcct agcactccat cgatagggcc gctatagaaa ccagcggcct tcaggcgcct
      541 ttggagtagc tggacgtcca ctttgctgag actctttgcg gaaccaatca aggcagttgc
      601 ttcggtctcg gtcgctggcg cggagagatc ggtattcgat gctgacaatt cgttgagaat
      661 tgaacacccg gcttgaactc gcaaaataac cgacctcgtt ttgtatccca atatgccgtc
      721 aaccggcccc gggtcgaaac cagcggcttt caatcgtgct tgcagttgac tgatctcctc
      781 tttactgaga gctttctgtg acgaaaaagc ccttcgacta ttttcattct ttacccgtgt
      841 gggagaatcg gagacgatcg gctgagccgg ttgttgctca acaataaccg gcgagctctg
      901 ctcgttttgg actgcaggct cggagacggc gctatagatc gcgcaaccat aactcaaaag
      961 gaacggaaca agcgctacaa gaatgtagct ttcctgccgc ctcgatttat tgcacttaag
     1021 cttgattact tccatgtctt gcctgtgcgc gagctagtag caaaggagat gccctgccgt
     1081 ggcacggaaa ctagtgatgt tactgttatt tcagagaatt caaaaagaat atgagttttg
     1141 ctcatggccg cttttgcttc gttgccgatg caatttgccc agaggcgtcc atcatgggag
     1201 ggtgtattaa tgataaattc ctttgccggt tgtaggctgc ggttttcgat cttatgttag
     1261 gaacatgccg gtcagtatga agcccttttc gctctacatt catatcccgt actgcatccg
     1321 taagtgcccc tactgcgact ttaactccca cgtggttgcg caaatccctg aacgtgatta
     1381 caccgatgct ttgatccggg agctggatca ttacgccagc tcgcccgatt ggcagggacg
     1441 aactgttcaa accatttttt ttggcggcgg cactccgtcg actttcacgc cttcgagctt
     1501 caacctcgtc ctggagcgaa ccttctcgtc gttcattatc gatccgaatt gtgagattac
     1561 cctcgaggcc aatccgggca gtgtcgatta cggctatttt tccggctatc gcgatgccgg
     1621 agtcaatcgc atcagcattg gcatccagtc ctttcaaccc cggctgctca gatttctcgg
     1681 ccgcgttcat accgcggaag aagggcgcaa agctctcgag gtggttaaga aggcgggttt
     1741 cgataacttc agtctcgatc tgatcttcgc taatccgggc cagacattga aggagttgga
     1801 aggcgatctc gacgcggcgt tagaatatcg atcgccgcac ttatcggcat ataacctgac
     1861 gttcgaagaa ggcacgccgt ttcatcacga atatcgctgc ggcagaatga ccacgctaac
     1921 cgaagacgaa gagatcgcga tggcggaact catcgaggat aaacttggcg cagccagatt
     1981 aaagcgttat gaaatatcga actatgcctt ggcggattgt cactcgcggc ataacgtaaa
     2041 ctactggcag ttgggcgatt atctcggcct cggcgccggc gcgcatagtt acttacaatc
     2101 aggtatggac ccgatggtga gaacgcgctg gagtaacgag aagaatcccg gacgctatat
     2161 gtcgcgcatc aacgaaagcg gaattgcgat caccgatcgg gaaaacatcg gtgaagacaa
     2221 agctgccagt gagtttctct ttttgggttt aagaatgata tcgggagttt cgtccgaagt
     2281 cttccgttca atttttggca gggctccggt cgagttctat ccaaagatag aaacctggag
     2341 agaagccggt ctgatggcgg aggaaaatgg caatctcagg ctcaccgcta aaggcttgtt
     2401 gctcgccaat tccatttttg ttgaattgat ctgaggcaag ttaatttgat gcagccgttg
     2461 cggtagtcgc gtcatcaacg aactgcaaga attgcaatac cgaggataat cttagaatct
     2521 ttcatcccgc agactcgcct ttcgtcagtc gggagtttga gtaccgagaa ggatcgttat
     2581 cgaggaggca atatatgtca acggcattgt ctacagaatt aaatcaactt cgtgtcgacc
     2641 acattggcag cctggtgcgg ccggctatgc tgaaagacgt gttcgcgcgc ttcgaccgcg
     2701 gcgcagcgtc gcgagaagaa ttaatccaag cgcaggacca ggcgatccgg caggtcatcg
     2761 cgagccagaa ggctcatggc ctgccagtcg ttactgacgg tgaatttcgc cggcatagtt
     2821 ttcaagaaag tttctctgaa tgcgtctcag gcttcgaggt gccaaacaac gtatctcttt
     2881 attatgagca gcgcgatcta aatttaaccc cgcttcaaag ggcagaacag aacttcgatg
     2941 aagccgggcc ggcaattatt acccggcgcg ctgcggtgcg acgcttaacg ctaatgcgca
     3001 atttgccttt ggaagaattt cgttacgccc aaagcatcac gcaggttccg gtgaaggtga
     3061 cggtactcgg ccccgaccgg attgcccaac gcttcaagtg ggaagactca caaaatgttt
     3121 ataaggatct cgacgatttc gtcgatcacg tggtggagat cgaaaggcaa atgatcgcgc
     3181 aactggtaaa tgccggctgc aaatacattc agatcgatgc tccgggatac accgcctacg
     3241 tcgacaaagt ttcgcttgag cgcatgcgct cgcgcggcga agatcctgaa cggaatctgc
     3301 agcgctcaat cgatgccgat aatgcgctga tcgaaggctt ccccggcgtg acgtttggca
     3361 ttcacatttg ccgcggcaac gctcgcacca cagatccgat taccggcaag ctggtgccgc
     3421 agtggcatcg ggagggatcc tacgaggcca tcgccgaacg gctcttcaat agcctcaaac
     3481 attatcggct gctcctcgaa tacgacagtg aacgcgccgg cggtttcgaa ccattgagac
     3541 tggttcccaa agagaaggtc gttgttttgg gtctggtcac gaccaagagc gcggatctgg
     3601 agagtgtcga cgagctcaaa cggcgcatcg aacaggcaag taagtatctg ccgctggatc
     3661 agctagccct gagcccccag tgcggcttcg gcggcattga ctccaaagtg atgagcgaag
     3721 aggagatgtg gcgcaaattc gaccggatcg tcgagacggc ggcgcaggtt tggagttagc
     3781 cgattattac gcgagttaca tctgccagca ttctcttgca tgcttgatgt ggcttttccc
     3841 tagcaattac cccagaacgt ccagcgggtt ggcgtgaacc gaatcatggg tcgagagtgg
     3901 atcgccattt gccggtattg aggatatttc ctgcgcaggc ggcgcacggc ttcggcatga
     3961 gcggcgccgc gatgaagaac ctttgccttg ccaccgatca aaacataagc gagttgcttc
     4021 caatcctcgc tgtagcgatc gatcaccaga gcggcctgcg gattcgcttg aatgttgatc
     4081 actcgcctga gcttcgccgg gctcgttcgc ttcggtttct cgtcaatcgg ggaatagaaa
     4141 tattttccat caaaaacaaa gcagatgggt ataacgtgag gctgattgtc aaggccagcg
     4201 gtagcgagat gggcaacccg cgcggcacgg atgaaggcga aaggtttctt tgcgagcgcc
     4261 atggagaacg ggagtattgg cgtaatggag tggtggagta cttaagttcg aagaacccag
     4321 tattccattg ctccagtgct cgaccacacc aatgtttata ttttaaactc tccggccacg
     4381 ttaggcgcga agatttcatc caccgtaagt tttcgcgcca gtaatccctg ctcatccatg
     4441 taacgaatca aatgctcaag caaatggcga ttggattcga ctccataagg ccaccaatcc
     4501 gggccgaaga tttctcttaa ttgttcgcgc tcccatggca gccatggaag agtgcaagcc
     4561 atggtattcg aaatgtgcat gttcttaatg gcgacgtctt tggcttcgca aaaagccttg
     4621 tacagactgc gcgccaccca ggtgtttttt tcgtaaactt ctctgcggat gaccagcaca
     4681 tgcataatcg ggaagatctt ggtccgttta tagtagtcga cttcgacatc tttatagttg
     4741 ggaaatagtc ttttgacctt cggcgagccc ttgacgaaac ttgaagggct gcgagccgaa
     4801 atcaaggcat cgatctcgcc gctctcgagc aggccgttca aggttttgtc atcggcaata
     4861 ggctcgagtt tgatttgggg cggcaaggta agtttgacac gttcttttcg gccggtttct
     4921 tcctgtcccc cggtgaacca ctgcatgtcg gaggttttaa cgccgtagtc gtcgttcaaa
     4981 aacccgcgaa tccacacggc ggcggtaatc gagtattccg gcgcgccgat tttcttgcct
     5041 ttgaaatcgg ccgggctctt aatgccggaa cctgtattga tgaacacgca agagtggcgg
     5101 aagaatcgcg acggaaacac cggaatcgcg acgaacggcg aattgcccct gctgatcatg
     5161 gtcacgtgat tggacagcga catctcggaa acgtcgaatt cgtggtgatg ccccatgcgc
     5221 caaaaaattt cttcggcctc aagcggaaca tagttgatcc gtatgccttc ggcttgcacc
     5281 gcgccggttt gcagcgcgaa ggtccggtcg taattgccgc aggcgacggt gagaaataat
     5341 ttttccatta agaagcctcc tgaagattta agacgaaatc taattagttt cccaacgcga
     5401 atagcgctgg gcgatcgcga tcagaatcac cgagataacc gagatgacca ggccgatggc
     5461 ggcgacccgg ccgcgcatgc cgtcaagata caggaagtaa agcagtggac cgagaggttc
     5521 ggcgccagga ctgtagagaa aaagtgtggc gctgaattca cgcaagaaga ttgttacgag
     5581 aataatccag ccggccatta cgccgggccg catcatcgga atcagcacgc ggcgaaatgt
     5641 cgccaggaaa ccggcgccgc agaccaccga agcctcttcg agctccttat gaacttggat
     5701 gatggtgctg ctgacggcgc gcagcccata gggaaggaag cgggtcacat aggcgatcaa
     5761 gataatccag atggtgccat aaacaggaat gggaaaatcg acatacgccc agagcagccc
     5821 cagagccatg gcagttccgg gaaaggccca cggtatgaag accaagcctt ccagcaatcc
     5881 gcgtccggtg attttcgttc gaaccgaaat gtaggacgcc agggaggcga gaagcatgca
     5941 aatggtggcc ccgaccaacg ccaggaacaa actgttaagg aaagctcggt gcactctcgg
     6001 cgttgcccag atgtaacgaa agtgatcgaa agttagattt tgccaggtct gccatgtggg
     6061 cacgtgataa taaggtaaaa tggcaacgag caggagcacc aggatcggca ataccaccag
     6121 tagtaccagc agtaaaaatg ccacggcggt ggccaagtag cgatatttcc ctaaatcgat
     6181 ctgatgcggc ctaaaccctt tccccgtaac cgtcgagaag ctctctaccc gcgaagtgaa
     6241 acgccggtaa agataaacaa acattagcgc gatcacgaga atgccaacgc cataagtcgc
     6301 agctaaattg tgattagttg gaaaagagcc caaagcttcg cgccaaatct tggtcgtgaa
     6361 cacttcaatg cgagcaggca gagcgataat tgccggcgta tcgaaactct ccacggcgcg
     6421 cacaaagttc aaagtccctg ccgccaatat agcagggcgg attagtggaa acgtgatccg
     6481 gcgcatgatg ccgaactcac ctgaacccaa agtcttcgcc gactcctcca gggacgggtc
     6541 catactcttc aatgcggcgg cgataatcaa aaaggccaac ggcgtcagaa tcaaaccctc
     6601 aacaaaaatc agcccggcaa gggaatagat gttgaaaggt cctcgctcta cgccgaaaag
     6661 ctgaatgaac agtccgttga taagaccgtt actcggattt aacaacatca cccaagagat
     6721 cgacaccagg attggcggca atatgtttgg aatgatcgca gtcagctcga aaaatcgtcg
     6781 cagcggtgca ttggtgcgaa tggtgatcca ggccagggtt aaagcgaaaa cgactgagag
     6841 tccggccgag ccgatgccga agataaatga attgagcagc aggggataag cttcaggatc
     6901 gctgtaggcc cgcacataat tctgtagtgt aaattgaccg ggaacgccca atggcgcgtc
     6961 agtgaggcta ccgtagaaga ggaaaactgt cggataaagg ctgagaaagg cgatgatgct
     7021 gccaacgaca ataaacaccc agctgatcgg ctgggtgaga aacgcaccaa cccgcgagcg
     7081 gctccacgcg cttgggagcc gttggccaac cgcgatggat cggtccactg acacctaggg
     7141 agcttaccgc agaaaaatct gctggtactc ctttttcttc tccgcgaatt ccttggagct
     7201 caagtcctcc atctcgacaa aaccgatctt gtcggccccg ggaagcggcg gaagcacgcc
     7261 cttgcgattg acgaattcgc cgagtttggc catggtgttc atgctttcgt tgtcgagaaa
     7321 atagtcgata aatgctttgc cggtactggg atgaggcgcc ttggctgcta gcgcgatata
     7381 gtgcccgtcc ccaagcattt tgggcaatcg gacatactcc agcggggcgc cccttttacc
     7441 gaacacgtac gcgtatttca cgtaggtaat tgcgatcgga gtctcaccgg tcatcactcg
     7501 ctctgccgac ggaaggagcg attcgaccag tatcggtttc atggccgcga gatcgcgaat
     7561 atacttctcg gccttctctt tgcccatgac cttgtgaaga cttgcgaccc actgggtcgt
     7621 ggtggtgtgc tgcgtcgggt ccggcatgac cagcttgcct ttgtatttgg gattgaccaa
     7681 atcctccagc gatttgggtg cgtcttcagt tttgacaccg gatttgttgt agacgacacc
     7741 aataatcaca ttgcgatatc ggggcccgag taccggatcg atggcctcct tcggaaaatc
     7801 gctccacgtc ggcgattcat atttctgaaa gaaattttcc ttctgcagca atcgcaaggg
     7861 attgtcattg gtaacgatca gatccgccac cgaccggcct gcgcgcgctt cgcttaatgc
     7921 gcgatccatc actttggttg ctgaagcgcg ccaatactca atctcgagac cggtcttctt
     7981 tttgaagcca ttcatgatcg cttccatggt gtccgattcg agagagccat aggcaatcac
     8041 tttgccttct ttttttgcgg cctcaatcaa ctttgcgtcc tgcgcagaag ctaaacaggt
     8101 aacgaaaaga aagctcgcta aaaccgctag cgaaagagtc gtattcttca gcatggtctc
     8161 tttctcctta cctttgaaat ggtggccgct aaagcgttcg aatacttcca gatttcaatt
     8221 tctgagtcaa gccgatggca gacccaacga cggtcgtttg tcgaggtaat ttgacacgct
     8281 taatcgcggg tcatataagg aaatttctct cattacttgc gaagggagtt tgatggatgg
     8341 ataagctcga tctactgata aagaatggcg aggtctggac cccgggtggc ttcgtcgagg
     8401 ctgatatagc ggtaagcggg gggaaaattg ctgcattagg gaaaccgccg gtgttaccgg
     8461 atacggccgc taacgtgatc gacgccaagg gtaaaaaagt tattccaggt ctgatcgaca
     8521 cgcacacgca tcatcgcgac ccagggttta cccataaaga agacataacc accgcgacaa
     8581 tggcagctgc ggcgggcggc gtgacattta gcgtcggcat gcccaacgtc aacccaccga
     8641 cgacctctgt ggagcattat cgcgctctcg tggcgcatca cagtaaaaat gcgattgtcg
     8701 attttaacca caatccttcc ggcacagtgc cgcgcgaggt tcctggactc gccaaagagg
     8761 gccctatcgc ttttaagatt ttcatggtca aagacacggg ccgggattat ccgcatatgc
     8821 ccggcatagg catcaataac aatggcgagc tcttcaagtg cttcgaaacc gtcggcgcaa
     8881 ccggtttgcc gctgatggtg catccgcatg atcaagatct catggatgaa atcgaagggc
     8941 gttactggaa agacgagaat cgaagtcctc aagcctatgc caaagcttat cgcgaccacg
     9001 acggcatcat ctgggatacc gccatggcga ctttgtttcg aatgcagaaa gcgatcggca
     9061 gcaagcttca catcctgcac atgagcaccc ctgaaggatt ggcgatgctg cgccgcgcca
     9121 aagacgaggg ccgtccttgt agctgcgagg tcaatccctg ggcgatgttt ctaggcacct
     9181 gggaaaatgt cgagaagctc ggtccctact gcctgggctt ctgggtgccg cccgcgcatg
     9241 tcgaggcgct ctgggaagcg atcaacgacg gcactatcga cgtgatcggc accgaccatg
     9301 cgccgcacac tagagaagag aaggaaaagg gctggcatga catgtggagt tgccccggcg
     9361 gcgagccgca aatccaggat tatctgcgac tttttctaac ggccgtgaac cagggaaaac
     9421 tgagcctcga ccgcctggtg cgcatttcgt catacaatgc ggcaaaactt ttcaacgttt
     9481 acccgcgcaa gggagtcatt caacctggct cggatgcgga tatggtcatt atcgacatga
     9541 acaaagagga agtgatcagc aacgagacca cctatactaa ggttggctgg actccttacg
     9601 atgggaaaaa gatcaaaggc gcgccgatcc gcaccatact gcgaggcaaa accgtaatgg
     9661 aagacggcaa gatcaccggc aagcctggcg acggacaact ggtctcgcct gtggtttaaa
     9721 ctccggtaaa gtgctgtggc ctggcgggaa taacaacaag ccgggacttc cgctttctta
     9781 acctgttggt tatggatccg tcggttccgg cacttcttcc gacgggtcga tcttgttaat
     9841 caccaatccg gaaatcagct tcggatagaa ataggtcgac ttttgcggca tcgtctcgcc
     9901 gctatcaacg attgttagga tatcggcgct ctttgtggca ttcagaagga aagctgcctg
     9961 ataatcttct ttttccaacg cttgcaacac cgcctcttca tcctgcgagt agcgaatggt
    10021 ttcgccgctt gtttgctgtt ccagtgaaag gcccaagatg tgctcgagta tcaaatggtg
    10081 cagcgtggat acttccaaat cgcgcagagg cgcgctgagg tccttggcca gccgttgcat
    10141 gatgcgtttg ttcttgagcc gtaatatcag gtagcgcgga tcgcgtttga agctggctcc
    10201 aataactcga tgcttcttgc ccgagctttt caaagctctc aagaacgagc tctttccttc
    10261 cgaagtcttg ggatactgct cgatgtaaaa ataagtctcc aaagcctctt ccagttgtaa
    10321 aaaagctttg ggctcgtatc cacgcacaag ccggtgagtc ggaagaatca ctacgctctc
    10381 atcattgatg ttggcgaaat gggtcatgat gtagttgaac gattcgcggc cggtcgatga
    10441 tcgatcgctg cgcatctgat cacggtatct gagcgtcgcc tcatagcgat gatgaccgtc
    10501 ggcgatcacc aaccgttgct ccttcatctg gcgctggatc tcacggatca aagccggatc
    10561 cgttatgcgc cagaggcggc actcatcgcc gtcatcgagc ttaacctcga gaaaagcctc
    10621 cttgccttca atctgttcgg acaaaattcg gttgatgctg cctttaggct gagagtaaag
    10681 cgcgaagatc gagctcaact gggcatgcga ggcaagcatg agttggagtc tgtcctgctt
    10741 gggagcctcg agtgtctttt cgtgcggccg aatctcatcg ccgggaaaat cttgtagttg
    10801 ggttaaggca tagaagcctt gacgttcttt gatctctccg ttcttcaacg caaacttgtg
    10861 gacggcgaaa tagatggccg gttgtttatc gcgctcaaaa atcccttccg cctgccagtt
    10921 tgcaaatagt tccgccactg cggcatatga atccggctcc cggtgaaaat ccagtcggac
    10981 gaaattatgg ggcgatttcc gatagagctt ttcttgctct tccggagaaa tcacgtcata
    11041 gggaggacta acaacccgcg caaggtcgcg gaattttttt tggttataga aaactccgtg
    11101 aaacggtgca attcgaacca tcctgcttac tttctggcat tgagtcttag ctcgcggccg
    11161 attgtcggag ttctcggagc tcaattctgc ttggcgacgc cgaggttcat cgcgccgata
    11221 ccttcaatct ccgatctgac gatatcgccc ggcctgagct tctcctggaa ttccggcgca
    11281 tcgggatttc ccgtgctgat caggtcgccc ggaaaaagtg ttgttacctg cgacaaatag
    11341 ctgaccagag tcggtatatc gttgaccatt gaatcggtcc tggcggactg cttgagctct
    11401 ccattgaccc acagcttcat tgatagttga tgcggatcgg cgatttcgtc tttagtagtg
    11461 atccaaggtc cgatcggcgc gaaggtttcg aaacctttgc gcacacaacg gctgcccgac
    11521 aggtccttgc cggagccgta gccgcgcgca gtaacatcga gcagaatggt atagccaaaa
    11581 atcgcctcat aggcgcgctc ttttgaaata ttgcgtgttt ctttgccaat gacgacgcaa
    11641 agttccagtt cgggaaaaat ggtttccgct ccatcaggaa tgataatgtt atcttccggg
    11701 ccgcaaatgc acgagggagg ttttagaaaa attcgttcga ggagctgttc cgccgtagct
    11761 gtgccggcgg cgcctctgcc gcgagcgtca tccaagccgc cggtaccgcg tttgtaattg
    11821 gtcgccgccg cccagatctt ggagggtttc tcaatcggcg ctttcaacat cttcgagtcg
    11881 agtttgctag gagtaagttt agacgcgtaa ctctcaatta gatctttgat gctgccgtat
    11941 ttagcaatca tctcaagcat cgagaagccc ttgggcaggg cactttcacg aactaacgtt
    12001 tcgcccagca gaatcagcgc atcatttttt acaagagcaa tctcattcgg tttgatgcct
    12061 gccaacttca tctcatgact cctttcgtgc tagccttgtg catatatcgc cctgcggaac
    12121 agttcaagcc gatcggaaaa cagttcagtc gatacaaatg ttcaaaaccg cgagagttga
    12181 tatcactggc gtcttgcgcg gccgtcttcg agcagtgctg atttattagg aacgcgttac
    12241 gccggcgcgc ccgttcacat aatttgcccc tccgccaact ctatggcata aaaaactgtg
    12301 acttcttcct ggttgcagct ctctttgcgc actgatccat tggcattgga cgcggtgtcc
    12361 aattttctca tagaacgtgg atctcccggg gtagtcatca agaaaaacca agtgcaggca
    12421 tactttgcgt gtccggagga ttcggcaacc atccgccggg acgtgcaacg gttcctgcgc
    12481 ggcatccgcg gcatgtatcc agaaatcggt gaggatcagc tgcgctggcg cgttctcaaa
    12541 gaccgaaact ggaattactc gtggcgacga tttttttcac cgcagaaagt aggccaggca
    12601 ttcttggtcg ccccaccatg ggctaaatta ccgccgcttg gacggcggca tctgatcgtc
    12661 atagagccag gcatggcgtt cgggaccggt actcatgcca ccacgagagg ctgcatggaa
    12721 tttatcgaga gggtcgccgg ttctctcggc ccaaaatcgt ttacagctct tgatgtcggg
    12781 accggttccg ggatcttggc gatcgcgctt gcgaaattag gcgcgagcaa ggtctgggcc
    12841 ttagacaatg atccggtcgc cttgaaagtc gcccgagaaa acatcgcgtg caatggggtt
    12901 cagcagaatg ttttttgttc cgcgggcccg ctcaaccgtc tacggagttt tccagttgtc
    12961 gtcgccaacc ttaccgctga aacgattatg agcttggctg agcctctggg aaagcgcgtt
    13021 gctcctgctg gctatttgat tctgtccggt gtcttacggc ccaagtcaga gcaactggtc
    13081 cgctactttg gtgcgaaagg cttcaggctg gtcaagctca aggaagagaa agaatgggcg
    13141 accttacttc tcaagcggaa gtaaccaatg gcccgttttt ttctgccgac gcagcagatc
    13201 caggatcgtc gcgctacgct catcggtgac gaattaaatc atctgcggcg agtattgcgc
    13261 ttgcgaacgg aagatcgtgt tgtcctgttc gatgacgccg gctgcgagca cgagggaatt
    13321 attcgcgatt tgagcgaaga ccgcgccgag attgaaatac ttcgctctta ccatgtcgat
    13381 aacgaatcgc cgctgaaaat cactcttgct ttaggtctca ccaaaggcga gaaactggat
    13441 tgggtcgtgg aaaaggcgac tgaattaggt gttcacacga tccttccgtt cttttccagg
    13501 tatacggtac caaagctaaa cgaaacaaaa atggcgcagc gttccgagcg ctggcagaag
    13561 atcgcggtta gcgccgcaaa acagtgcggt cgcgctcgca tcccgaagat tctggcgctg
    13621 acagaatttc gcgatctcct caaaacggta tcgggcgatg cgctgccact gctattctgg
    13681 gagaaggagt gcgagcaacg attgtcggag ttaaaggaaa cgcgaacgag tattagcgag
    13741 gttgtcgtaa cgatcggacc cgaaggtggt tttagcgcgc aagaagcagc gctcgcgctt
    13801 gatcagggct tccaatcggt gcggcttggc cgccgcatcc tgcgtgctga aacggctgcc
    13861 gttgcggcgc tcgcggcggt ccaactcttg tggggtgatc tagcctaggt tagtgttggg
    13921 cgatcggcgg tcaatcgcgg caaggtgcaa acggtcggaa caatctatta tgcgttgtcc
    13981 cgcatgtgat tccgtgatca cgcataacac gggagtatgc agtcgctgca acgcgccgtt
    14041 ggatcctacc ggcctccggc gcgattcaac cgatgaagag gttcgtctcg actcggacca
    14101 ggagaaaaca gacttacgtg atgagagctc ggatcggatg gaaacctaca tcccgccgag
    14161 tggtaaagcc gacgagcctc cgcttgggag cataaatcat caagcgcctg acaaccggct
    14221 gtggggtggc tttattcggc gagcctgcgc cttcgctgtt gactgggcga taatcttgct
    14281 gctgggcttg gtgatgtttc tgatgtcata catcggttac aaagtcgggc tttcagccca
    14341 cggtagatcc gtgacgatgg aaaactcgag tggtttatat tttattttga cctgggcctc
    14401 ggtggcctta tctgccgcgt acttcgtgat cttccatagc ttggacggta gaacgattgg
    14461 taaatggata tttggtttgc gggtcatagg agccgagaac ggcgacgtca cctccagcca
    14521 agccttttta cgttggctcg cctctgtggg ttttgcgccg attcttttgg gtttcttgtg
    14581 ggtgatttgg agccgcgaga aaagggcctg gcacgacatc atcgcccaga cgtgggtgat
    14641 caggcagtag gcgcgtcctg gcttggttag aacatctatg aagccgttaa aaattggggt
    14701 cgtcatggac ccgattgaaa agatcgacat cgacaaagac acaacctttg tcttgatgct
    14761 tgaagcgcag cacagaggcc atgaaatcta tttcatggag atcgacgatc ttttcgcccg
    14821 cggggggacg ccctgcgcac ggtatcgacg ccttcacctg gcccgggccg tgccacacta
    14881 tcatctaact cagatcgaca cccgacctct tgaagaccta gacgtggtgc tgatgcgcaa
    14941 agatccgccg tttgatatga aatatttttt tgcgactcat cttttgagtt tgatcgatga
    15001 gaagaagtgt ttcgtgatga acaatccgag aggattgcgc gatgccaatg aaaaactcta
    15061 tgccttgcgt tttcctgaac agatcccaca gactctggtg agcagcagca tcgaaagact
    15121 taaggctttt atggacgaat taggcggcga aatgatcgtc aagccgctcg atggtagcgg
    15181 cggcagcggg gtattctatc ttcatcaaca ggatcgcaat accaactcga tcttggaggc
    15241 cgccaccgac aatggccgtc tcatgatcat gggacaacgc tacctacctg aaatccggca
    15301 aggcgataaa cgaatcatag tcctgaacgg taaaccgctg ggcgcggtct tgagagtgcc
    15361 gctggaaagt gaaatccgag gcaatatcca tgtcggcggg cagtgcgtca agactgagtt
    15421 aaccgcgaga gatcgcgaaa tctgcgaagc tcttgctagc ctgctaattg ccgacggttt
    15481 gtatttcgtc ggcttggatg tgattggcaa ttttttgaca gaagttaacg tgaccagccc
    15541 caccggaatt caagagatca acgcgctcga cagcgttcga ttggaaagca gcgtgatcga
    15601 ctttgtcgaa gagcaagtcg ggagaattca gtagatcagt atcttagaac accaggagca
    15661 accgtccacc tgcgtggtca ttctgtaagc ttcaagcccg atatttagat acggcttgcc
    15721 gcgctctagc gagtcagcaa ttgctctacc agccaaatgg ctgcaatgga ttctgtattg
    15781 cttcctacgt aatcgcgatg attgtctaag cgtcacgcaa gacaatgccg gcgtgagcat
    15841 tcagcgcgct tgcggattaa gtaggcagcc atactcggcg ccggcttcgg atacttgcgc
    15901 cggtgacttt gtactcggcg atcactgcgt cctcaatcgc cttcagcatt ctgtcatcag
    15961 tggggtaggc gactctccga accttcccgt cttgctgttt cacctctggc atgcaaactc
    16021 ggtagccggt cgctgttcgc agaagtttaa attcagtgat cgacagacag tcgtcgaagg
    16081 tcacgtctgc atagccgcgc agggtccctt ggaccgtacg gtaaatctta acatttgtta
    16141 tttgcatgtt cgctcccgat gtggtggcgg agcttatacg ttgggcaagc ctacgagtca
    16201 agacagcgcg catcgctgcc cgcacccgaa tgggctt
//
LOCUS       DASUVS010000317         8478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_787264, whole genome shotgun sequence.
ACCESSION   DASUVS010000317 DASUVS010000000
VERSION     DASUVS010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8478
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787264"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1701..4667)
                     /locus_tag="VF182_17040"
     CDS             complement(1701..4667)
                     /locus_tag="VF182_17040"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6438846.1"
                     /translation="MFRLSRVRSVRTHFLLIFLGTAIPLIILTAMAVFYVGRAEFRSA
                     KTGLEDTARALSGAVDRELQASIRTLEALATSKLIDQGNLTDLHQNVISILPSQPGWR
                     TVIMRDASGKDIFHSSFAFGTPRPETVQPVSFDEVINTLRPTVMNYFVGPVTGPTIGV
                     RVPVVREGELKYVLTATIDAVRIDQILHEQRLNDGWYAAVFDRERVQVASSTANSEAN
                     RGRKPGPLISQIPDEADAAWISGPNRAGVDSYGAFVKAPISGFYVGIIVPHSEMSRTL
                     LNSVWFISLIGVVAGAAGLFLITFYGRLVRHCTTYLIDLAHMIGKGEPVEIVAPAPVT
                     EVNLITSAMAEASALLQTTKAERDKADAERDRFEISLQEVRDNLSQRNAELRIVTDTM
                     AVGVARLDKNQKYLWVGKRLCEWLGKSPSEIVGHSIKEILDPELYALIEPRIRAVLSG
                     QLIEFETEWRLRLGRRWMHASYSPTYAADRAVDGWVAVITDIDKRKRAEEALDHLARF
                     PLEDPAPVLRANPEGVVMYFNPAAEKALAELPSANREQLPAALAEAVSQALRDGHRRE
                     LEMYFGERLFSFLVVPILDRQYANLYGRDVTVQKAAEAALIEADRRKDEFLAMLGHEL
                     RNPLGIISSGVQLLRKLGPPDPNVIELREMIARQVTHTSRLLDDLLDVSRLSRGKIQL
                     VKGPCDLRDIVQKTAEDYVRELRENGLRLELALTDEPLRTAADSTRLAQALNNLLQNA
                     CKFTEPGGTVWVSLDAANQTHAIIKVRDNGMGMDPEVLGWVFEPFRQVDESVDRSRGG
                     LGLGLALVKGIVELHGGEVAAASAGRGQGSEFTIRLPLDQNHTPAAVVRVNTPEPVRR
                     RILVIEDNLAGAYSMRMVLENLGHTLETAHDGAAGINAAQRFRPDVVLCDIGLPVLDG
                     YSVARAIRQNPRLDKCLLIAISGYARDEERAREAGFDAHLLKPVDFEKLEALLTVRTG
                     GSMMPRTKNGNASAES"
     gene            complement(5863..7110)
                     /locus_tag="VF182_17045"
     CDS             complement(5863..7110)
                     /locus_tag="VF182_17045"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX6438847.1"
                     /translation="MSTVYELDLRAIAPGELFSTLYQTYRKLDPGTRLRAIVDTNPRG
                     SYMGFVEAGVPHRITRVGDGEWEWSATRSAWEPMGNGPGVHHIGISPDGQRLYAVDRE
                     RTVFMFDAARQRLTESTCVNKGTSHLAVHPRSGHVYACERSTDSMIRLDGGTLRLRGR
                     IATGESPNLPAAAEDGSAVILPGRNGVITVAYDDEEVRAVNLPVGGQPSASTISHDGR
                     RAYIPDTARNLLVSIDLKEEKVLAETPVGDGPSHPIVTPDDAMVCIANSRSHDISLVD
                     AQTFGVIVTIPSGEAAHQTTLTADGKVLLVANFFEDSVSIVDLPSRKRVAVVRVGPYP
                     HGLDMAPGNRYAIATHFGDPWISVIDVPERKVAALVAAGLGSSHTSFSPDGERAYVAS
                     SIANTITVIDLNSLEAIAQIGVA"
     gene            7744..8268
                     /locus_tag="VF182_17050"
     CDS             7744..8268
                     /locus_tag="VF182_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438848.1"
                     /translation="MKKSQFVSTLVTLLIFASSAIAQDKRHDNTLVKFKGGIGAIPIS
                     NVAVDATTGAITVNRNMVRGVNSPGQIWTIKDLEARIRLNGDIKVEGNGLVLAGGNSI
                     GRATGQSVFATLFCGTIEHSTTTTGVPLATDGDFKIDDVLVPTPPTDCADPVLLIRTA
                     GSGNWFAAGIPDLD"
     gene            complement(8265..>8478)
                     /locus_tag="VF182_17055"
     CDS             complement(8265..>8478)
                     /locus_tag="VF182_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438849.1"
                     /translation="NALRESGEAYSSHFTVEMSLLGSKMRLWNAIAQVVGKLAWFDPP
                     TALVHCQLTQLGTRELTALTNHSGQG"
BASE COUNT         1934 a         2420 c         2198 g         1926 t
ORIGIN      
        1 ggcgccgccc gaggcttcac tgtaagcctt gagcagtacc gcgggcgtcg ccccctaggc
       61 ctcaaagcag aagctgccgg caagaagcac cagaaagaat aataatctca cgtccatacc
      121 tccttagacg ttcgagttaa cacgcaaagc ttaagggagt cttaacaggc aaaaaaattt
      181 gcgacggctt gagcgtccgc tgatcaagca aaaaaggagg ggtccgggaa aatttcaact
      241 gcacgagcgc taacgccgcg cttcgagaca cggatcgcgc tcagcacgcg acgcgcggag
      301 atcgcggcgg ccgtcctctc gtctcggcca acgtgaaagt cattttctcc tatcctctct
      361 ctcaatcgaa tttacatatg atccatacat gggcccttgc ttgactgccg tgcgtatcga
      421 gagattcaga aaaccccgaa gggctatggg tttatcgatt tacgggagtt aactttgcta
      481 tgtcgcttca gtgagattgc cgattttcag caagcctacc gcaggaagcg gttgtcgcgg
      541 gattcgcatc cgagactttc cgaattctct cttggatcga tttgaactga gcgtagcgat
      601 taaacggctg gaacgcttta aaccaaccct cgttggtcag aagcgatcgc ggtcggcagt
      661 ttgaattttg tcgagaatgt gccgagaacg aatctggaga caaagccgcg catcacgatt
      721 gaaagtggcg gtatctatca gctcagggag cagactgaaa gttatgggcc caattttatt
      781 ctcaaaatga agtgctaagt cagaacacga gattttggaa cgaagattct gaacttactg
      841 caacttagct tggtgcgagc ccggagttaa ccctggtact gaagaacaat cactctggag
      901 aacaattccg gcgtgatctt tgagcgcgcg cgtgaagcag gtgcgcgaaa aaattttctc
      961 ctgcggcatg attatagatt ttcgacttca acgtcctgag cgatccactt gaccgtgtct
     1021 ctgtccagct tgaaagtcgg tccagggcgg ttcactacct ccaatgcttc agcaatgcgt
     1081 cgtttccggc ccttcgagct gaaatgcttc gctttgagtt ccgcgaacct gttttttttg
     1141 gggtttcttt tggtgcgcgt ttggccatgg cagtatccta agccatccca ggaaccgttc
     1201 caaattgaca ttaacctcgg tacagccagt aacatggcaa ccaacagccg cttctcttca
     1261 ctctgcttgg gtacaatccg gacaacgcgt tcgccaacgg caataatgca tgaaagcctg
     1321 acacggggct accctgctag gctaccctcc catatcgcta agcttcgagg ctcttgctac
     1381 gagtccggga agcgaagcgg tgcgtggctc aaatacaagg tcaacaagcc gcaagcgctc
     1441 gtgatcggcg gctaaacgcc aggcaacccc gctcgatgct aatcgtcggc tctaggtggt
     1501 gagaagccga tatttgcaag taaggtccgc aacggctttg tccctcgcct gcggcgtgag
     1561 gtctgggcaa agttgaagca cttacaaact gacgcttgcc cattcactaa cctgctggag
     1621 aagaaaccaa ctgaataggc gctcaccccg cgatgtactt caattttagt cttcacctcg
     1681 cgaaatgagt gtactgacac tcagctttca gcggaagcat tcccattctt ggtccgtggc
     1741 atcatcgagc cgccagttcg cacagttaac agagcttcga gtttctcaaa gtccaccggc
     1801 tttaggagat gcgcatcaaa accggcttcg cgcgcacgct cttcatcccg ggcgtagccg
     1861 ctgatcgcga tcaatagaca cttgtccagt ctcgggtttt gacgtattgc ccttgcaaca
     1921 ctataaccgt caagtacggg cagcccgata tcacaaagca cgacatcagg ccgaaatcgc
     1981 tgtgccgcgt tgattcccgc cgctccgtca tgcgccgttt ccagagtgtg gccaaggttt
     2041 tccagaacca tacgcatgct atacgcaccg gctagattgt cctcgatcac aagaatccgg
     2101 cgtcgaaccg gctccggcgt attaactcgt accaccgcag cgggagtatg gttctggtca
     2161 agcggaagcc gaatcgtaaa ctccgagcct tgtccccggc ccgcgctcgc tgccgccact
     2221 tcaccgccat ggagctccac aatccctttc accagcgcaa ggccgagtcc taatccaccg
     2281 cggctccgat ccacactctc gtcgacctgt ctgaaaggct caaataccca accgagcact
     2341 tccgggtcca ttcccatgcc gttgtcgcgc acttttataa tagcgtgcgt ctgatttgca
     2401 gcgtccaaac tgacccagac cgtcccaccc ggctctgtga atttacaggc gttctgcaac
     2461 agattgttaa gcgcttgtgc aaggcgggtc gaatccgctg ccgttcgtag cggctcatca
     2521 gttagagcga gttcaagtct taagccattc tctctcaatt cgcgaacgta atcctcggcg
     2581 gttttctgta cgatatcacg caaatcgcat gggcccttga ccaactgaat ctttccgcgc
     2641 gacaagcgtg agacatctag cagatcatcg agcagacggg aggtgtgagt cacttgtcgt
     2701 gcgatcatct ctcgcaactc gataacattc ggatctggcg gaccaagctt acgcaacagc
     2761 tggacgccgc tgctgatgat ccccaaagga ttccgcaact cgtgtcccaa catcgcaaga
     2821 aattcgtcct tgcgtcggtc ggcctctatc aaagctgctt cggcagcctt ttgaactgtc
     2881 acgtccctgc cgtataaatt cgcgtattga cggtcgagga taggaaccac gaggaacgag
     2941 aacagccgtt cgccaaagta catttccagt tcgcgtcggt gcccatcgcg tagcgcctga
     3001 gagactgctt ccgcaagggc cgcaggtagt tgctcgcgat tggcgctcgg gagttctgcc
     3061 aatgcctttt cggcggcagg gttaaagtac atcaccactc cctcagggtt cgcacgcagg
     3121 accggcgccg ggtcctccaa agggaaccgc gctaggtggt ccaatgcctc ttccgcgcgc
     3181 tttcgcttgt ctatgtcggt tataaccgcc acccatccgt ccactgctcg atctgccgca
     3241 tacgtcggag agtaactggc gtgcatccag cggcggccta atctaagtcg ccattcggtc
     3301 tcaaactcga ttagctgacc ggacaacaca gcacgaatac gaggctcgat cagcgcatag
     3361 agttcgggat ccaaaatctc ttttatcgag tggccgacga tctcggatgg agactttccg
     3421 agccattcgc aaaggcgctt gccgacccag agatatttct ggttcttatc aaggcgcgca
     3481 acccccaccg ccatagtgtc cgtaactatt ctgagctccg cgttacgctg ggacagattg
     3541 tcccgtacct cctggaggct tatttcgaat cgatcccgct ccgcgtcggc tttgtcgcgc
     3601 tccgccttgg tcgtttgaag tagagcggac gcttccgcca tggcactggt aatgagattg
     3661 acctctgtca caggcgctgg ggcgacaatc tccactggtt cccccttgcc tatcatgtgc
     3721 gcaagatcga taagataagt tgtgcagtgg cgcacgagcc gtccatagaa ggtgatcaag
     3781 aaaagacccg ctgcgccagc taccactcca atgagtgata taaaccagac ggagtttagg
     3841 agagttctgg acatctcact atggggcacg attatgccga cgtagaaacc tgaaatgggc
     3901 gctttgacga acgcgccgta ggagtcgaca ccggcccggt ttggaccgga tatccaggct
     3961 gcatcagcct catcggggat ttgcgagatc agcggacctg gtttgcgtcc gcggttggct
     4021 tcactgttcg ctgtgctgct tgcaacttgt acacgctctc gatcaaaaac cgctgcatac
     4081 caaccatcgt ttaaccgctg ttcatgcaga atctggtcta tgcgaacggc atcgatcgtt
     4141 gccgtgagga catatttcaa ctcgccttcc cgaaccactg gaacgcggac tccaattgtc
     4201 ggcccagtga ccggcccaac gaagtaattc atcactgtag gccgaagagt attgataacc
     4261 tcatcgaaac ttacaggctg gaccgtttca gggcgtggcg tgccaaacgc gaaagaagaa
     4321 tggaaaatgt ccttgccgga ggcatctcgc atgatcactg ttcgccaacc cggctggctc
     4381 ggcaaaatgc taatcacatt ctgatgaaga tccgtaagat ttccttgatc gatcaatttg
     4441 gaggtcgcga gagcttccag tgtcctgatg ctagcctgca gctcgcgatc aaccgcacct
     4501 gacaacgccc gcgccgtatc ctccaggccc gtctttgcgc tccgaaactc agcgcgaccg
     4561 acgtaaaaga cggccatggc cgtcagaatg atgaggggaa tcgctgtccc aaggaaaatt
     4621 aataggaagt gggtacgaac ggaacggacc cgcgacaacc tgaacattga aactctcttg
     4681 gggcgacaac cactcgccgc gcggtcgaaa gagaacatcg cacctgcgat ctttagaagt
     4741 gttaaagaaa tatccagcgt agtggaatga tttcggctct gttagctatc gcgcccccgc
     4801 ccccggattg tcaagcacaa tctgagacag tgaacagtta ccggtccttg tggtcggata
     4861 ttgccgccat ttccgcccgc gtttgggaag ctacgtgaca acggctcctg aatgctattg
     4921 cgtcatagaa gaatgacaat gatcactagt tgtccattct gagattttta gctctattta
     4981 tcgccctagt attcacttcc agcttttccg cgtttctcat actgctcgcg gatctcacgg
     5041 ccaagtctgg cctgtacttc tgtctcagca aatgttcgga tggtgtccct ggaggttaac
     5101 ggactatggg atgaagcgag catctgttca attttctgcg ccgcggtctg acagtgtagt
     5161 tctgctacga agcagatcgc gtgcggtgtc ttggcgacat tgaatagtca ctcccggagc
     5221 tgaaacacca ctgagtctcc acttcattat ggagactctg agaatcgccc atgcagcaga
     5281 gcttttgctg gcggattgca gtaagcatgg cgcctcttgc ccatcgacaa caatctcgac
     5341 cagttcagtt gacatactgt ttgccctcgc gtttacggtg acgaccggcg gactggctac
     5401 agtcagatta tttgcttagt agatggagca aaaaatgcac cagtggaaga ccgtgctaat
     5461 agggtctcac cagacattga tttcttgagg ttgcgaaaat cacaatatca aagccgcact
     5521 agcaagaatc tactacgtcc aatttggcgt agtgaacgca cgccgttcgg cgccataccg
     5581 cttaatcatt tcctcaggta caaagattac attcttgcta tgtatcgccg catgaatgcg
     5641 aacggggatc gcctgacaga gaaccggaga aaagtcttgt cactagagag aatcgcacaa
     5701 tgactcatgc gcaaaggata gatagtgctc gtctcggttg gattcgaacc gcttaatgga
     5761 cgaccccgga gttaacccaa cagtgctcgg acactaagcc cggcaggttt cttcatcggc
     5821 gccttgcagg cgaaagtagc ctgtccctct tgccaagcgc gcttatgcca cgcctatctg
     5881 cgcgatcgcc tcgaggctgt tcaagtcaat aaccgtgatg gtattggcga tgctactggc
     5941 gacataagcg cgctctccgt ccggcgaaaa actggtgtgc gaagaaccga gtccggccgc
     6001 caccagggcg gcgactttgc gctcaggtac atcgatgacg ctgatccacg gatcgccgaa
     6061 atgagtggcg atcgcgtaac ggttcccggg agccatgtcg aggccgtgcg gatagggtcc
     6121 cacccttacc actgccacgc gcttcctact cggtaggtcg acgatgctca ccgagtcttc
     6181 aaaaaaattc gctaccaaaa gaactttccc gtctgccgtc agcgttgtct gatgagccgc
     6241 ttcaccgctg ggaatcgtta cgatcacgcc aaacgtttgc gcgtccacca atgagatatc
     6301 gtgcgagcgt gagttggcga tacaaaccat cgcatcgtct ggcgtgacga tcggatgtga
     6361 cggaccatca cccaccggtg tttcagcgag caccttctcc tctttcagat cgatgctcac
     6421 caggagattg cgcgccgtgt ccggaatata ggcccggcgc ccatcgtggc tgatcgtcga
     6481 ggcgctgggc tggccgccga cgggcaaatt caccgcgcga acttcctcat catcgtaggc
     6541 tactgttatc acgccattgc gtccgggcag gatcacggca ctgccgtctt ccgccgctgc
     6601 cgggaggttc ggactttcgc cagtggcgat ccgtccgcgc agccggagcg ttccaccgtc
     6661 caatctgatc atgctgtcgg tgctgcgctc gcacgcatag acatgtcccg agcgcgggtg
     6721 cactgccagg tgagacgtcc ccttgttcac gcaggtgctc tcggtcaatc gctgccgggc
     6781 tgcatcgaac atgaacacgg tgcgttcgcg atccacggca tacagccgct gcccgtccgg
     6841 agagatgccg atgtggtgca cgccgggccc gttccccatt ggctcccaag cgctgcgtgt
     6901 cgcggaccac tcccactcac cgtcgccgac ccgggtaatc ctatgcggaa cgcccgcttc
     6961 gacgaatccc atgtaggacc cgcgcgggtt ggtatcaaca atcgcccgca aacgcgtccc
     7021 cggatcgagc ttgcgatacg tctggtaaag cgtcgaaaac agctccccag gcgcaatcgc
     7081 gcgaagatcc aattcatata cagtgctcat agagaccttt cttcagccgt cggattctgc
     7141 gcagaactcg atgccgggaa atgaaatcat caatctctct ttagcctctc tttagtatcc
     7201 tgtcacgcag gttcacagca aatttcatcg tcattccggg gacgctggca taccagcgtg
     7261 acccggaatc gaaaatattc gaatacccct gaatgcggct ttaaccggaa ttagaaatcc
     7321 tcaccgccga ttggatcgag ttggagcctt tgttttttta gtcttggcca aaaaactggc
     7381 gattgacatc gttttggttg taggcctctc tcattatttt actctttccg gcgcaactcc
     7441 agccgacata atgatcggac gtccatgcga gcacaacggc ggcaaggcgt ctgtcgccgt
     7501 ccggcgcttt aggtgtatcc gctagatcca ttagctctgt agcgggtagg cagggtcgaa
     7561 ccacgccaac ttaccacaac ttaattggcg cgggaattca ggaattcaag ttcagaacgt
     7621 ctggtcgaac ccgaacgggt aaccaaaatc ctcggggaga ttgacatcac tagcaccgca
     7681 gcggataaat cataatcgat gttccacggt cggtccgtca cggaaaaaaa atggaggtgc
     7741 tttatgaaaa aaagtcagtt cgtttctacg cttgttaccc tgctgatctt tgcttcatcc
     7801 gccatagcgc aggataagcg ccatgataac accttggtga aattcaaggg aggaatcggg
     7861 gctatcccga tctcgaacgt tgccgtagac gcaactacag gcgctattac tgtcaatcga
     7921 aacatggtcc gcggggtcaa ttctcctggt cagatctgga cgatcaaaga cctagaggcg
     7981 aggataagac tcaatgggga catcaaggtc gaaggaaacg ggttagtgct agctggcggc
     8041 aatagtatcg gaagggcaac aggacagagc gtttttgcca ctctgttttg tggaaccata
     8101 gagcacagta ctacgaccac gggcgttccc cttgcgaccg acggcgactt caaaatcgac
     8161 gacgtgcttg tgccgacacc gccgactgat tgcgcggatc ctgtgctcct catccgcacc
     8221 gccggcagcg gcaactggtt tgccgccggc attcccgacc tcgattagcc ctggccgctg
     8281 tggttcgtca acgctgtcag ctcacgcgtt cccaattgcg tgagctgaca atgaacgaga
     8341 gcggtaggcg ggtcgaacca cgccaactta cccacaacct gagcgatcgc gttccataag
     8401 cgcatttttg aacctaagag gctcatttcg acggtaaaat ggctgctgta agcctcgcca
     8461 gattctcgta acgcattg
//
LOCUS       DASUVS010000318         3602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_787452, whole genome shotgun sequence.
ACCESSION   DASUVS010000318 DASUVS010000000
VERSION     DASUVS010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787452"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            487..1539
                     /locus_tag="VF182_17060"
     CDS             487..1539
                     /locus_tag="VF182_17060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6438850.1"
                     /translation="MERDIHLVGVAVVGSKYEIVAINKVFCEKIGYEKADLAGASLID
                     IVTPAEQEKCKRLLVEAGKGKDAAYNSEMHFVARNGETVGTKVTVTTAGDPSETANIL
                     LTIEDFDQPCARKTSTHTGGLTRMEKSAEIFMHELRNQMHGISLLTRLIEQESTRRGL
                     LEDDPLVILHQDLKLRIKDVGTLLQDLSHLGESMKLKLQPTDFAKFVTGLLSSDQASQ
                     MAQQIRIDCDIAADLPLIQLDRRRFKQAWLNLFKNAVEAMPSGGTITVRLYSSEAYAC
                     LEISDTGTGVPDGIDIFEPFTTTKAQGTGLGLGIVREIVSAHGGVVSYRSTPQTGASF
                     IVSLPLGASKSDCEKS"
     gene            1737..2483
                     /gene="aqpZ"
                     /locus_tag="VF182_17065"
     CDS             1737..2483
                     /gene="aqpZ"
                     /locus_tag="VF182_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HEX6438851.1"
                     /translation="MNRYLAELLGTFVLVFASCGSAVLAGDKIGFLGISFAFGLSLLA
                     MVYAIGPISGCHINPAVTVGLLLSKKIDPKSAGGYIAAQIVGAILAAAILLVIAQGAP
                     GGYDPSVAGFAANGYGEHSPGKYSLLSAFLVEVVLTFFLVYTVLGATDIRTPVGFAGV
                     AIGLVLTLIHLVGIPVTNTSVNPARSIGPAVFVGGWALSQLWLFIIAPIIGAVIAAGL
                     YAATHKPSEVISAREAERALAAEQAARKSA"
     gene            2620..2931
                     /locus_tag="VF182_17070"
     CDS             2620..2931
                     /locus_tag="VF182_17070"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6438852.1"
                     /translation="MLKQIFTLSAVGLIAFYLSGCQAITGESLGQNIDDTTITTSVKS
                     KLAVDKASSMTRVGVETTRGTVQLTGVVDSQATKNKAAEIAQSVDGVKNVVNNLQIQS
                     K"
     gene            3230..>3602
                     /locus_tag="VF182_17075"
     CDS             3230..>3602
                     /locus_tag="VF182_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase F"
                     /protein_id="HEX6438853.1"
                     /translation="MIFIGLLIGALAAAVPVAAVAASVTALKLETQVVKGDWEEILKR
                     GTIRVAVPYSRTLFFNDRGNQMGLTAEIIREFEQWLKKKYPKRSRPITVLAIPTTRDK
                     LFGKLRNGYAEIAAGNLTITAE"
BASE COUNT          883 a          908 c          967 g          844 t
ORIGIN      
        1 gagataagta tcattgaagt tcacgtgcga agtcacgcgc tttttcatat catccctcgt
       61 tgctgctgat tgtggacatc agcggtgcgt tccgccccgc aggcttctat cttccttcga
      121 attcgctgca cgcgctgccc ctttgggccc gatagtccgt ccttcgatcg tctatatcga
      181 ttgtcactag cgataccgtt aaatattgtg ggatctgctg aatgtcgtgg gctagaatcc
      241 cattgtccat gcgggctctt tctaagttac atagagaagt acttatttcc taaagagaat
      301 cagaagttat gagatgcacc caaaaaaatg agtagttgcc ttctatgcgg cattgctctt
      361 gcgctaatcc cattctagcg gtctcgctct ggtgtgcagc tgcgaccaga aatgatcggc
      421 atctccctaa gcggtgatag cggaagctta tatcgatatt aaaaaccggg aaggagcacg
      481 cgttatttgg agcgcgatat tcatctcgta ggggtagcgg tcgtcggaag caaatacgaa
      541 attgttgcca tcaataaagt gttttgcgaa aaaattggtt acgagaaagc cgatctagca
      601 ggagcaagct tgatcgatat cgttactcct gccgaacagg agaaatgtaa gcggttactg
      661 gtagaagccg gcaaaggcaa agacgcagcg tataactcgg aaatgcattt cgtcgcgaga
      721 aacggagaaa cagttggcac caaagtcacc gtcacaaccg ctggcgaccc gagcgaaacc
      781 gcaaatattt tgctaactat tgaagatttc gaccagccgt gcgctcgcaa aacatcaacc
      841 cacacaggcg ggttaactcg gatggaaaaa tcggccgaaa tcttcatgca tgaactccgg
      901 aatcagatgc acggcatttc gcttctgact cgcctgatcg agcaagaaag cacccggcgc
      961 ggcctgctcg aggacgatcc attggtaatt cttcaccaag atctaaaact gcgcatcaag
     1021 gatgtaggta ccttactgca ggaccttagc catctcggcg aatcgatgaa gcttaaactt
     1081 cagccgactg acttcgctaa attcgtgact ggattgctta gctctgatca ggcctcccaa
     1141 atggcgcagc aaattcgcat tgactgtgac atagccgccg atctgccgct aatacaactc
     1201 gaccggcgca gatttaaaca agcatggctc aacctcttca agaacgcggt ggaagccatg
     1261 ccatccgggg gtacgatcac agttcggctt tacagctcgg aggcttatgc atgtttagag
     1321 atctcggaca ccggaactgg cgttcccgac ggcattgaca ttttcgagcc gtttacgacc
     1381 acgaaagcgc aaggaaccgg cttgggattg ggcatcgtgc gcgagattgt ttcagcgcac
     1441 ggcggtgtcg tttcgtatcg aagcacgccg caaacaggcg cgagctttat agtttctttg
     1501 ccgctggggg cgagcaaaag cgattgcgaa aaaagctaaa atatattaac ccagggcaga
     1561 agcgctccac tttagctcgt cggcagggcg tttctaagga gttccggggt gaagccaaac
     1621 gagatgatgc atcattcatc tcgcacgaag aaaagtttct gaccgcaatt cctccggtac
     1681 ttgaaaggct ccgccaatca aagtcggtag cggccaaatg catggagatg caaaccatga
     1741 acagatatct tgctgaacta ctcggcacgt ttgtgctggt ctttgccagt tgtggcagcg
     1801 cagttttggc aggagataaa atcggctttc tcggcatctc gttcgccttc ggcctgtcgc
     1861 ttctcgcaat ggtctatgcc atcggaccga tttccgggtg ccatataaat cctgccgtga
     1921 ccgtcggatt gcttctgagc aaaaagatcg atccaaagtc tgcaggcggg tatatcgcag
     1981 cccagatcgt cggtgcaata ttggctgcgg caattttgtt agtcatcgcc caaggggcgc
     2041 cgggcggtta tgatccgtcg gtcgcaggtt tcgccgccaa tggttacggt gagcactcgc
     2101 caggcaagta cagcctcttg tcggcttttt tggtggaagt cgtcctgacg ttttttctgg
     2161 tttacaccgt tctcggagcc accgatatcc gaacgcctgt tggtttcgcg ggagtagcca
     2221 tcggcttggt cctgacactt atccacttag tcggtattcc cgtaactaac acctcggtca
     2281 atccagcgcg cagtatcgga cccgccgttt tcgtcggtgg ttgggcgttg agccaactat
     2341 ggctttttat tattgcgccg atcattggtg cagtgatcgc tgcagggctc tatgcagcga
     2401 ctcataagcc gtctgaggtc attagcgcga gggaagcgga gcgagcgctg gcagcggagc
     2461 aggccgcacg gaagtcggct taggccgaac agaacgaaaa tcgtttctgc gccaatacgt
     2521 gattcctatt tgatgggatt gtgagcggtg acgtgacaac cattaacaag cgatgcgagg
     2581 attgcttcaa gcaactagag aacagaaatg gagggaacta tgcttaaaca aattttcacg
     2641 ctttcggcgg tagggttgat cgctttttat ctttcgggat gccaagccat caccggtgaa
     2701 agcttaggac agaacattga tgacacgact attacaacgt cggtcaaatc caagctggct
     2761 gtcgataaag ccagctccat gacaagagtc ggcgttgaaa ccacacgggg taccgtgcaa
     2821 ttgaccggag ttgtggatag ccaagcgaca aaaaacaaag cggccgaaat agcgcagagc
     2881 gtcgacggcg tcaagaacgt cgtcaacaat ctgcagattc agtccaagta acctgtagct
     2941 aggtgaacca tggatcttgc acaggcgccc tgataggctg gatctctcta cgctcgcaat
     3001 cgtattttcc cttgtcgcgg ctgaggtaag tattctacct agctagccgt taaatcctaa
     3061 tgattgaaag tgcctaggca ccgtcgcggt cccgcacgcc gacagcacga tagaattacg
     3121 gtggcgcatc ggctcgtgat cacgccgtgg cgaatcagat cggcaccaaa ccccctcgag
     3181 ttccgtatat aacagtctga atatagcagt ctaacaggag gtagtggtcg tgatcttcat
     3241 cggtcttttg atcggcgcgc tagcagcggc cgtgccggtt gcagcggtgg cagcttcggt
     3301 caccgcgctg aagcttgaaa cgcaagtggt aaaaggggac tgggaggaga tcttgaagcg
     3361 gggaactatc cgcgttgccg ttccttatag ccgaaccttg tttttcaacg atcgcggcaa
     3421 ccaaatggga ttgacagcgg agatcatccg tgaattcgag caatggctga agaaaaaata
     3481 cccgaagcgt tcccggccta ttaccgtcct tgcaataccg acgactcgcg acaagctgtt
     3541 cggcaagctg cgcaacggct atgcggagat tgctgcgggg aacctgacga ttaccgcgga
     3601 gc
//
LOCUS       DASUVS010000319        13790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1879734, whole genome shotgun sequence.
ACCESSION   DASUVS010000319 DASUVS010000000
VERSION     DASUVS010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13790
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879734"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VF182_17080"
     CDS             complement(<1..978)
                     /locus_tag="VF182_17080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6438854.1"
                     /translation="MRLGLCFDGFYSVQEMIELARLAEETRIESLWMSDHLCFRDSLT
                     TSMALLASTQRIKVAPAPMSPYSRAPIISAMALATMEEFAPGRVIASPGTGNAAALKE
                     AGIESPRPLKTMREYVDILRRLLNGDTVDYQGDMFRLRGPKMGFVPSTPIPMYVTAVR
                     PRMLQLAGEIGDGVLLSGGCSPAYIARCIDEIRTGATKAGKLITHLDVAGFITAAVAG
                     NAREAIDANKLFLAYIFRNTHHAENIRLGGGKVDQEGLAAAVGERDWDAAKKYISDDV
                     VFAHSIAGTADDCRRQLQAFVKGGLNLPVLLPMGTQEMRKEVARMAARLV"
     gene            complement(1071..2177)
                     /locus_tag="VF182_17085"
     CDS             complement(1071..2177)
                     /locus_tag="VF182_17085"
                     /inference="COORDINATES: protein motif:HMM:NF014381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HEX6438855.1"
                     /translation="MAVRKVAVLGAGNGGCAAAADLTLRGYEVRLFSRSDATLLPIIK
                     QGGITVWEAGEEKFAAPYFHSPHIVPVIDGVDLIIVAAPAVAHEYLARSMAAHLRDGQ
                     RILLNPGHTGGSLHFAHVLRCAGNKAAVRLCETVTLTYICRMIKPAHVEIYRRTTNLR
                     CAAFPARENDDLVCEIQSIYPNVTVAQNVLETGLSNINAIMHPAGMLGNVGWIEKTGG
                     NFLYYREGITPAIAAWIEKIDKERLQIVNRLGLKPMPFINIFYQAGLTTSEAQASGSV
                     YRAIHESEPNFKIKSPADLNHRYLHEDVGYGLVPMAEIGRLVGIETPVMNSLITLASV
                     ANGVDYSREGLTLEKMGLADAGPANVAAILRNGF"
     gene            complement(2310..3467)
                     /locus_tag="VF182_17090"
     CDS             complement(2310..3467)
                     /locus_tag="VF182_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX6438856.1"
                     /translation="MKISSIETIPIRLPTRRVHQWASLTTPIGVYVIVKLHTEDGPIG
                     LGEAPVLKDWGGDYGRYFGETPQSTMHIINDILAPALKGQDPSCFEAIHSLMDKAVKG
                     YPYCKAAIDAALYDVVGKALNVPAYQLLGGCFRERVSIAHSLGLMEIDKAVDEAVEAK
                     AEGVRTIKLKGGVDQKRDVELVKQIRKAIGPDLHICVDANQGYPTAKAAVRIIEAMEE
                     YDLFYMEQPVEGIDHMAEVAHRVNTPIMADESAWTAQDVLEIVYRKAADIISIYTTKP
                     GGMFKAKKVAAVAEAAGLPCNINGSVETGVGNAANLHLAASTGVVTYACVVPVSSPKE
                     KAKKGIAGIYYQDDIISEPFAFDNGDIIVSPKPGLGIEVDEDKLEHYRIKA"
     gene            complement(3473..4585)
                     /locus_tag="VF182_17095"
     CDS             complement(3473..4585)
                     /locus_tag="VF182_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX6438857.1"
                     /translation="MDQEIIVRLKQRIVDQGLDALIAISPENVTYVSGFVVPSQSLMR
                     WRHAICVVTADARVSMVAIDMEATTVKAHAGIDDLRIYREFSDDPMEKLAETLRDLDL
                     ARGNLAIEMEYLPAKDFAILQRFLPDAEWNPGDAIFNKARQIKTSGELSLLRSLSKLT
                     DKAIGDTLMQAKPGISELELAGRLLTELFAGGAENYKLMIIASGERSQFPNVGPTERT
                     LQKGDLIRMEIFGQKNGYLAGVCRTAAVGRATSEQEKIWANLIECKYLVMDMIKPGAR
                     CREIYQTFLAKFSELGFEPISFVAHGIGLHLHEEPYMGRYGDAIVEPGMVGAFEPLVY
                     IPGRFGMQNKDMFCVSQTSCELLSDYTPADTLLTVG"
     gene            complement(4752..5870)
                     /locus_tag="VF182_17100"
     CDS             complement(4752..5870)
                     /locus_tag="VF182_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX6438858.1"
                     /translation="MSLADKIEINGPKKILALDGGGIRGILSVEVLAKIERMLRDKLG
                     RGNDFVLADYFDFFAGTSTGAIIAACLSWRMSVDRIRDFYLQNGREMFDRASLLNRYY
                     RHKFEDQKLSARLRQEFGAETTLGSEKLGALLMMVMRNASTDSPWPISNNPRAKYNDC
                     ARADCNLDLPLWQLVRASTAAPTYFPPEVVRVGPKEFIFVDGGITMYNNPAFQAFLMA
                     SMEAFNLNWPVGENNLLLVSVGTGTSPDANADLRPDEMNVLYNASSIPSALMAAALNE
                     QDFLCRVFGKCLAGDPLDREVGDLIGTKGPVQPRLFTYLRYNAELTRHGLTSLGLPDV
                     EPKDVQQLDSVEHIPDLQAVGSAVADRKVSLDHFAGFV"
     gene            complement(5884..7845)
                     /locus_tag="VF182_17105"
     CDS             complement(5884..7845)
                     /locus_tag="VF182_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HEX6438859.1"
                     /translation="MAHHAFVAMPFGIKEDIDFNAVFGELIKPALEAAGFEVFRADEE
                     MRAGNIRTDMFQELLLADLVVADLSIDNPNVWYELGVRHALRKRGVIQITCRRDQMPF
                     DLLTERSLRYRIAIKSAAAVPDPQYLEDDKKRLTEVATQTINAWYGRKVSPVYHLLPY
                     LEEPDWKSLRIEEAKEFWEQYENWADRIEIARRGNRPGDILVLAGEAPTRVFHVEALQ
                     KAGRALLSLGQFKFALAQYEEALAIRPRDLETRRQKCLVLGRLKKYDDAKECIEDLIN
                     EFPEDAESWAMRGRVEKDGWVDCWKQDQRAVGDMRREAEIETGILHEAINAYATGFRK
                     DPAHYYSGINAITLLHLLAHLSAADDVKADVRAEMEGGVRWAIRGALERNPKDYWARI
                     SLAELEVLVGARQAIDAAYKNAVAVADKDWFKLNSSRQQLELLRLLGFRPAQVEAGVA
                     ILDRALSRLNPPDKTWTPQQVVLFSGHMIDAPDRPEPRFPADKEKIAAAAIAAALEKL
                     NAGADDVALCGGACGGDLLFAEAALERGLKLELRIAFDEPTFLQKSVSFAPGNWTERY
                     FKVKSHPNCTVLVMPEVLGGLPPGVDPFARLNLWQLYTSLAWGPEKVRFVCLWNRKGG
                     DGPGGTQHMFETVQQYSGRVYVIDTTTLW"
     gene            8264..8437
                     /locus_tag="VF182_17110"
     CDS             8264..8437
                     /locus_tag="VF182_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438860.1"
                     /translation="MNRKELEMRIWGKTIASAKKKGIISEEEAQFLLDEYLKEFKVKN
                     GWDGVSHFIPRVF"
     gene            complement(8734..9198)
                     /gene="grdA"
                     /locus_tag="VF182_17115"
     CDS             complement(8734..9198)
                     /gene="grdA"
                     /locus_tag="VF182_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_081590103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(9070..9072),aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HEX6438861.1"
                     /translation="MELRGKKIAILGERDGVPAHTIEQAIENLGAQVVYATTQCFVUT
                     AAGAVDLEVQGRVKQLAEEYGKNDLVVLLGAPNVDAARVQFETMTRGDPTYAGPLGGV
                     ELDLPVYHVFEPEMKAIIDPDRYSDLIETLELGLEADAIVEAIQQSRAGENQ"
     gene            complement(9334..10776)
                     /gene="grdC"
                     /locus_tag="VF182_17120"
     CDS             complement(9334..10776)
                     /gene="grdC"
                     /locus_tag="VF182_17120"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF040746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HEX6438862.1"
                     /translation="MSNFVPVIKAASSVLIHTPGLTRYGSKPHREISANPARLADLNR
                     NLRSFQQAQAYLPNQVFIGQQAPEKLSSVSRPWFDQFAALSEERKSFGSIVDEKLFLA
                     LIALADPFKHVALLDHFWEETVEPLRQLPICRSLVSATPNVVDSTKLKTVIAQDGGLP
                     LFAGSDFEPVGFIADGHHEDESLSATVLLENLCGKVSAALSLQEIFGGNPSLKKQDVD
                     FVLGCSEEAVGDRYQRGGGNLAKAIAEFSGCDMASGFDIKDFCAAPIPAMVTAAALVQ
                     SGVAENIVVVGGSSLPKLGMKFLYHLEKKIPVLEDMQAAVAVWVGRDDGKSPIINLAG
                     VGRHPVSAGASLDQQLKSLVVDPLNRLELGVPAIDKFVTELHNPEITLPANGGDVAER
                     NYRMLGAVLVKAGKLAKSDLALFMKEKGLPGYAPTQGHIASALAYVPHAVQELTHGQF
                     KRCFLFARASLFLGQMTQLSDGMSVMLERQ"
     gene            complement(10968..12272)
                     /locus_tag="VF182_17125"
     CDS             complement(10968..12272)
                     /locus_tag="VF182_17125"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HEX6438863.1"
                     /translation="MRLELADYPVTEIRLGRKFEYRAGRLEVDQPALRETILGDRRIA
                     EASLAVVCPGDKVRITGIRDIVEPRVKSSGNGQVFPGILGPVQAVGSGRTHRLSGMTV
                     IATAAYEGTVRAGLGVQRSALLDTWGAGAAASKFGGLLHLVLIMKLRDGLSELDAHTA
                     IQKAEFQVARRLAEATIGSDATNLEVFDLSQGHPDLPNIVLVQGCLTDDHHVHSGVSY
                     YGLPIRESLSTVVHPNELLDCAMAVNATRGIGYFPITWDWQNHPLMFALYRDHGRRLN
                     FGGMILQRIRYETFHGKEVIAQNAAQLAVTLGAQGAVVTWLGSGNAFVDVMLTVRACE
                     RRGIATVLVSYEYGGKDGVDSPLLFYVPEADAVVSTGSRDRWLELPEAERLVGPYDQV
                     QILSYPGAPVAQAQGKLTFDARDMIIGGVDNWGKESWICNAY"
     gene            complement(12279..13727)
                     /locus_tag="VF182_17130"
     CDS             complement(12279..13727)
                     /locus_tag="VF182_17130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(12558..12560),aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HEX6438864.1"
                     /translation="MSVCSSNRSSPRRAIDVYARDDSVLIECITAKEDFTMTTIVHVV
                     NQFFAGLGGEEKAGMSVGVIEDTAGAARGLQSQLGAEIKVACTIYYGDNYFHEHNEEA
                     INAILEAVRSHDAQALVAGPAFNSGRYGMSCVEICHAVAAALDIPCVTAMHEENPAIG
                     TYRDMAHARVFCLPTTETAAGMTDALRALAKFVSRLTGAETIGPALQEGYFSRGMRRL
                     EKTDRTGADRAIDMLLKKINQEPFTTEIPMEEWDDIAPAPPLDDLKARNIAVITTSGV
                     VPWGNPDRFKTYRNTHWRKYNIAELKELEPGKWEAVHGGYNVAYMNKNPHYGVPLDAL
                     RSLEAEGVIGAGKLYPAYYVIPGNQGSPAVMRRVGKEIAADLKKDNVEGVLFVATUGT
                     CSRSGAVMSKELEKAGLPVALVSAMYPVAEQVRANRIVKGVSIPHPCGDPNLPQDLDA
                     RLRREIVQTALKALEAEVTGPTVFTPDMAVSG"
BASE COUNT         3049 a         4020 c         3592 g         3129 t
ORIGIN      
        1 gacaagccgc gcggccatgc gcgctacctc ttttctcatc tcctgagtgc ccatcggtaa
       61 gagcacgggc aagtttaacc cgccttttac aaatgcctgc agctgccgtc ggcaatcatc
      121 cgcagtgcca gcgattgagt gggcaaacac cacatcatca ctgatatatt tcttcgccgc
      181 atcccaatct cgttcgccca ccgctgccgc taatccctcc tgatcgactt tgccgccccc
      241 gagccgaata ttctcggcgt ggtgagtgtt gcgaaaaata tatgcgagaa aaagcttgtt
      301 ggcatcgatt gcctcacgcg catttcctgc aaccgcggcg gtaataaaac ccgccacatc
      361 taggtgagta atcaatttgc cagctttcgt cgcgccggtt cggatttcgt caatgcaacg
      421 cgcaatatat gccggcgagc acccgcccga gagcaagacg ccatcgccga tttcgcccgc
      481 tagctggagc atccgcgggc gcaccgcagt gacatacatc ggaatcggcg tcgacggcac
      541 gaatcccatt ttggggccgc gcagtcggaa catgtcgccc tgataatcaa cggtgtcacc
      601 gtttaacaat cgacgaagga tatcgacgta ttcacgcatc gttttcaggg ggcggggcga
      661 ctcgatgccg gcttctttta gtgcggcagc atttcctgtc ccggggctgg caatcaccct
      721 ccctggcgca aattcctcca tcgtcgccag cgccatggcg gagatgatcg gcgcgcgtga
      781 gtacggactc ataggcgccg gagcgacctt aatccgctgc gtcgaggcaa gcagagccat
      841 tgatgtcgtc aacgaatctc tgaagcagag atggtctgac atccaaagcg actctattcg
      901 agtctcttcg gcaagacggg ccagctcgat catctcctga actgaataaa atccgtcaaa
      961 gcataagccc aaacgcataa agccctcttt ctaatcttga cgcgggtctc atagatttcg
     1021 cctggaaatg tgagacatcg gagcagccgc aggctattct tacaatcgtg ttagaaccca
     1081 ttgcgcaaaa tcgccgctac gtttgctggc ccggcatccg ccagtcccat tttctccaaa
     1141 gttaaacctt ctctcgagta gtcgactcca ttagccaccg aagctagcgt aattagcgaa
     1201 ttcatcaccg gggtttcgat accaaccagc cggccaattt cggccatcgg caccaagccg
     1261 taaccgacat cttcatgaag gtaacgatga ttgagatcag ccggagactt gatcttaaag
     1321 ttaggttcgc tttcatgaat cgcgcgatag actgaaccgc tggcctgtgc ttcagacgta
     1381 gtgagccctg cttgataaaa gatattgata aatggcatcg gtttaagccc gaggcggtta
     1441 acaatctgca agcgctcctt atcgatcttc tcgatccaag ccgcgatggc cggggttatt
     1501 ccttcgcgat aatacaaaaa gttaccgccg gttttttcga tccagccaac gttgccgagc
     1561 attccggccg gatgcatgat ggcgttgatg ttcgacaaac cggtttcaag cacgttctga
     1621 gcaactgtga cattggggta aatactctgg atttcgcaga ccaaatcgtc gttttcccgc
     1681 gccggaaaag cggcacagcg gagattcgtc gtccggcgat agatttcgac gtgggccggt
     1741 tttatcatcc ggcaaatata cgtcagagtt acggtttcgc acagtctgac ggccgccttg
     1801 ttgccggcgc aacgcaaaac atgggcaaaa tgcagcgagc ctccggtatg gccgggattt
     1861 aagagaatac gttggccatc ccgcagatgc gccgccatgc ttcgcgccag atactcatgc
     1921 gcaaccgccg gcgctgcaac aataatcaga tcgaccccat cgataaccgg cacgatgtgc
     1981 gggctgtgaa aatagggcgc ggcaaatttt tcctcgccag cttcccaaac cgtgatgcca
     2041 ccctgcttga taatcggcag cagagtcgca tcggaccggg aaaacagccg aacttcatag
     2101 ccgcgcagcg tcaaatcagc cgccgccgca cacccgccgt tgccggcgcc gaggaccgct
     2161 actttacgaa ctgccatagt aagcgagaaa agtttgcaac gctaacgatc ttttacgatg
     2221 acagcggata acgctattcg tgttcgccgg tgttcgtgct cggtcggctt taggtgtgac
     2281 gttttcacgg atacgaattc ggttggccgc tacgccttga tccggtagtg ttccagttta
     2341 tcttcatcca cttcgatacc gagccctggt ttgggtgaaa cgatgatatc gccattgtcg
     2401 aacgcgaagg gctcgctgat gatatcgtcc tggtaataaa ttcccgcgat ccctttcttc
     2461 gccttctcct taggggaaga aaccgggacg acacaagcgt aagtaacaac tcccgtggat
     2521 gcggccaagt gcagattggc tgcgttaccc actccagtct ccacagatcc atttatattg
     2581 cagggcaatc ctgccgcttc agcaaccgcc gcgacctttt tagctttgaa catcccgccc
     2641 ggcttggtgg tgtaaatcga tataatatcg gcagcctttc tgtaaacaat ctcgagcacg
     2701 tcttgcgctg tccaggcgct ttcatctgcc atgatcggtg tgttgacgcg gtgcgccacc
     2761 tcggccatgt gatcgatacc ttccaccggt tgctccatgt aaaagagatc gtactcttcc
     2821 atggcttcga ttattctaac tgcggccttc gcggtaggat aaccctggtt ggcatcgaca
     2881 cagatatgca aatcgggacc gatggctttt ctgatctgct taaccagttc gacatcccgc
     2941 ttctgatcga cgccgccttt gagtttgatt gtccggacac cttcggcttt ggcctcaacc
     3001 gcttcgtcta ccgccttatc gatttccatc agcccaagac tatgcgcgat gctcacccgt
     3061 tcgcgaaagc aaccgccgag cagctggtac gcaggaacgt tcagagcctt gccgacgacg
     3121 tcgtaaagcg cggcgtcgat ggccgcttta cagtaaggat atcctttcac cgctttgtcc
     3181 atgagagaat ggattgcttc gaagcagctc ggatcttggc cttttaatgc cggcgcaaga
     3241 atatcattga tgatgtgcat cgtgctctgc ggcgtttccc caaaatacct gccataatcg
     3301 ccgccccagt ccttcagcac cggcgcttca cctaggccga tcggaccatc ctctgtatgc
     3361 aatttcacga tcacatagac accgatggga gtcgtcaaac tggcccattg atgcaccctg
     3421 cgcgtcggca gccgaatcgg gatagtctcg atggagctaa tcttcatgcg tctcaaccga
     3481 ctgttaatag cgtgtcggcc ggtgtgtagt ccgacaacaa ctcgcaactt gtttgcgaaa
     3541 cgcagaacat atccttgttt tgcatgccga atcgaccggg aatataaact agcggttcga
     3601 acgcccccac cattccgggt tcgacgatcg cgtccccata acggcccatg tagggctctt
     3661 cgtggagatg caggccgatg ccatgggcga caaagctaat gggctcgaac cccaactcgc
     3721 taaatttggc aagaaacgtt tgataaattt ctctgcagcg agcgccgggc ttgatcatat
     3781 ccattaccag atacttgcat tcgatcaggt tcgcccagat cttctcctgt tcagacgttg
     3841 ctcgcccgac ggccgctgtg cggcacactc cggccagata accgttcttt tggccaaaga
     3901 tttccatgcg aatcaaatcg cctttctgca gcgtgcgttc agtgggtcca acgttaggaa
     3961 actggctgcg ttcgccggaa gcaatgatca tgagcttata attttctgca cccccggcaa
     4021 acagctctgt caatagtcgg cccgccagct ccagctcgct gataccgggt ttggcctgca
     4081 tcaaggtatc gccgatcgcc ttatccgtca gcttgctgag cgatcgcagc agcgaaagct
     4141 cgccgcttgt ttttatctgg cgcgctttat taaagatcgc atcgccggga ttccattcag
     4201 cgtcaggaag aaatctctgc aggatagcga aatctttggc gggtaaatat tccatctcga
     4261 tggcgagatt gcctctggcg agatcaagat ctctcaaggt ctcggcgagt ttctccatcg
     4321 gatcatcgct gaactcgcga tagatgcgca gatcatcaat gcccgcatgg gctttgactg
     4381 tggtcgcttc catatcgatt gcgaccattg aaacccgagc atcggcggta acgacacaga
     4441 ttgcatggcg ccagcgcatc aaagattgcg agggcacgac gaagccggag acataggtca
     4501 cgttctcggg agagattgcg atgagcgcgt ccaatccctg gtcgacgatg cgctgtttca
     4561 gacgaacgat gatttcttga tccatgatcg cggttcgaat gttcgagact ctaaattaag
     4621 tggagtgcta tgaatgcccc ggagaatagc tgctgttatc tggagcgtca agaagaagag
     4681 ccgagtgctc ggcgtcaagc cggatatttc ggcgagcgct gagagggcca accaggtatg
     4741 ctcaaggacc ttcagacaaa gccggcgaaa tgatcgagcg aaactttcct gtccgccacc
     4801 gccgagccga cggcttggag atcgggtata tgctcgaccg agtcaagttg ctgcacatcc
     4861 ttgggctcga cgtccggaag cccgagactc gtcaaaccgt gccgcgtcaa ctcggcattg
     4921 tatcgcaggt aggtaaaaag ccgtggttga actgggcctt tggtaccgat caaatcaccg
     4981 acttcgcgat cgagcggatc tccggcaagg catttaccaa acaccctgca cagaaaatcc
     5041 tgctcgttca gcgccgccgc catcaaagcc gagggtatgg agctggcgtt gtaaagcacg
     5101 ttcatttcat cgggtctcag gtcggcgttt gcatccgggc tagttccggt cccgactgaa
     5161 acaagcaaca gattgttttc gcccacgggc cagttcaggt tgaatgcttc catgcttgcc
     5221 atgagaaagg cctggaacgc cgggttgttg tacatcgtaa tgccgccatc gacaaagatg
     5281 aactccttag ggccgacacg caccacttcg ggcgggaaat aggtcggcgc ggcggtactg
     5341 gcacgaacca gctgccagag cggcaaatca agattgcaat ccgcgcgagc gcaatcattg
     5401 tatttggctc gtggattatt ggatatcggc cacggcgaat ccgtcgaggc attgcgcatg
     5461 accatcatca gcaatgcacc taacttttcg ctccccagcg tcgtctcggc gccgaactcc
     5521 tggcgcagcc gagcagagag tttttgatct tcgaacttgt gccggtagta acgattcagc
     5581 agcgacgctc tatcgaacat ctcccggccg ttttgcaggt agaagtcacg aattcgatcg
     5641 acgctcatcc gccacgagag acaagcggcg ataatcgcgc cggtgctggt gccggcaaag
     5701 aaatcgaaat aatccgcgag aacaaaatcg ttcccccgcc ccaacttgtc gcgcagcatc
     5761 ctttctatct tcgccagcac ttcgacgctc aggatgccgc ggatgccgcc gccgtcgagc
     5821 gccagtattt ttttcggtcc attgatttcg atcttatctg ccaagctcat agccgacctc
     5881 tcgtcaccac agagtcgttg tgtcgatcac atacaccctg cctgaatact gctgcacggt
     5941 ctcaaacata tgctgggttc cgcccggacc gtcgccccct ttgcgattcc aaaggcaaac
     6001 aaagcgcacc ttctccggcc cccatgccag cgaggtgtag agctgccaaa ggtttaggcg
     6061 cgcgaaagga tctactcctg gcggtaaacc gccaagcact tcgggcatga ccaagactgt
     6121 gcaattggga tgagatttta ctttgaaata gcgttccgtc cagttgcccg gagcgaagga
     6181 gaccgacttc tgcaaaaacg tcggctcgtc gaaggctatc cgtaattcaa gtttgagtcc
     6241 gcgctcaagc gccgcttcgg caaacaacaa atctccgccg catgcgccgc cgcagagggc
     6301 tacatcgtcc gccccggcgt tcaacttttc cagcgcagcc gctatcgccg ctgcggcgat
     6361 cttttcttta tcggcaggaa agcgcggttc aggccgatcc ggagcatcga tcatatgacc
     6421 actgaacagc acaacctgtt gtggcgtcca ggtcttatct ggtggattga gccggctcaa
     6481 cgcgcgatcc agaatcgcca ctccagcttc aacttgcgcc ggacgaaaac ccaggagtct
     6541 cagcaactcg agctgctggc gcgacgaatt gagtttgaac caatctttat cggccaccgc
     6601 aacggcgttt ttgtatgccg catcgatcgc ctgtctcgcg cccaccaaaa cttccaattc
     6661 ggctagactg atgcgcgccc aataatcttt ggggttcctc tcgagagcgc cacggatggc
     6721 ccaacgcact ccgccctcca tttccgcgcg cacgtcggct ttcacgtcgt ctgccgccga
     6781 caagtgagcc agcagatgca gcaaggttat ggcgttgatt ccggagtagt aatgcgcggg
     6841 atctttgcga aatccggtgg cgtatgcgtt gatggcttcg tgcagaattc cagtttcgat
     6901 ctccgcttcc cggcgcatgt ccccgacagc tcgttggtcc tgtttccagc aatccaccca
     6961 gccatctttt tctacgcgcc cgcgcatcgc ccagctttca gcatcttcgg gaaattcgtt
     7021 gatcagatct tcaatgcatt ctttggcatc gtcatatttc tttagtcggc ccagtactag
     7081 gcacttctgg cggcgagtct ccaaatccct aggccggatc gccagcgcct cttcatactg
     7141 cgcgagagcg aatttgaatt ggccaagcga aaggagcgcc cggccggctt tttggagcgc
     7201 ctcgacatga aacacacgag tcggcgcctc gccggccaat actaagatat ctccgggacg
     7261 attgcccctc ctggctattt ctattcggtc cgcccaattt tcatattgtt cccaaaactc
     7321 tttggcttct tcgatccgca gagacttcca atcaggctct tccaagtagg gtagcagatg
     7381 ataaaccggg ctcaccttgc gtccatacca agcattgatc gtctgagtcg cgacctcggt
     7441 aagccttttc ttgtcatcct ccaggtactg cggatccgga accgccgcgg ccgacttaat
     7501 ggcaattcgg taccggagtg aacgttcagt aagaagatcg aagggcattt gatcgcgccg
     7561 gcaggtgatc tgaatcacgc cccgctttct aagcgcgtgg cgcactccaa gctcgtacca
     7621 gacgttggga ttgtcgatgg acaagtcggc aaccaccaga tccgcgagca gcagttcctg
     7681 gaacatgtcc gtgcgaatat tgccggcgcg catctcttca tcggcgcgaa aaacttcgaa
     7741 tcccgcggcc tccaaagccg gcttgatcaa ctccccgaac acagcattga agtcgatatc
     7801 ttccttgatg ccaaagggca tcgcgacaaa tgcatggtgc gccatatcgt ttcacctcgt
     7861 ccgcggcggt atgcgatgtc ggcccagtac tgcacgcgcc tgcttgcgtg cgcgtgtcca
     7921 tcttcgctcc aacattccgg cgtaactgga agaaatccct cagtttagat aaaggttctc
     7981 aaccagcccg tcagtatagc ctttttgata gagaaacgcc cgccaattcc aacaacatga
     8041 ctcaaggcaa cccaggctca agtagcatga cgcgatcggc gagagagcga taatttagga
     8101 tttcccgcaa attctgtatc cactgcatat gacaactggt tttacctacc tacacgatca
     8161 atggagaggc aaccggtcgg gagtggaggg gaaacgagaa atttgcgctt ggctttccag
     8221 caaaaagttg ctaactaatt cagattccaa cttggaggtt acagtgaaca ggaaagagct
     8281 cgaaatgcgc atttggggca agacgatagc gtctgcaaaa aagaaaggaa ttatctcgga
     8341 agaggaagcg cagtttttgt tagatgaata tcttaaagaa tttaaggtca aaaatggttg
     8401 ggatggcgta agtcatttta tccccagggt cttttgaatc tcgaagcctc agtcagatgc
     8461 catatttgcg aacaaaattc tcagtgttcg ctttgttgat gacttctccg aaatttgctg
     8521 ctagtaccgc gcgctgttac ggttttcaga tcgagtaaac gtttggttcg gtaatcttcg
     8581 gttctgcttt cagccgtcga aagcacgcgt ttagttagag ccgacagttc taagccgcgg
     8641 gtcgttgttt gttccaactt aaccgctgga tcgcctgatt gctttgtctc gatcgcagac
     8701 ttccctaatc gcttgcatgg cactctactg cgcctactgg ttctcccccg cgcgggattg
     8761 ttgaatcgcc tcaacaatcg catcggcttc gagacccagt tccagggtct cgatcaggtc
     8821 actgtaacga tccggatcga taatcgcctt catttccggt tcgaacacgt gatacaccgg
     8881 caaatccaac tcgacgccgc cgagcggccc ggcataggtt gggtcgcccc gggtcatggt
     8941 ttcgaactga acgcgcgccg catcgacgtt gggcgcgccg agcagcacaa ccagatcatt
     9001 cttaccgtat tcctctgcga gttgtttgac tcgaccttga acctcaaggt ccaccgcgcc
     9061 ggccgcggtt caaacaaagc actgagtggt ggcgtaaact acctgggcgc ccagattttc
     9121 gatcgcctgt tcgatggtgt gggcgggtac cccatcgcgt tccccgagaa ttgcgatttt
     9181 tttgccgcga agctccatta aagtttcctc tcaatcccat ttaacttgac gttcagccct
     9241 cgtatccttg cagttccagc gtcactgacg tcgcatttca tctgtaatta ctgctgttcc
     9301 aaaataaccg acgtgcccgt ttgatctcgg tgattactgt cgctccaaca tcactgacat
     9361 gccatcactt agttgagtca tctgccccaa gaataagcta gcccgggcaa aaaggaaaca
     9421 gcgcttgaat tgcccatgag tcaattcctg aactgcgtgc ggcacataag ccagcgccga
     9481 ggcgatatgc ccctgggttg gcgcatagcc gggcaatccc ttttccttca tgaacagcgc
     9541 aagatcgctt tttgccagct tgcccgcctt gaccaggacc gccccgagca tccggtaatt
     9601 tcgttcggca acgtcgccgc cgttggcggg caacgtgatc tctgggttgt gcaattcagt
     9661 gacaaattta tcgatcgccg gcacgcccaa ctcgagccgg ttcaacggat cgacaaccaa
     9721 tgatttaagc tgctggtcca atgaagcgcc cgccgacact ggatgacgcc cgacgccggc
     9781 cagattgata atcgggcttt taccatcgtc ccggccgacc caaaccgcaa ccgccgcctg
     9841 catgtcttcc agcaccggta tttttttttc aagatggtaa agaaatttca tgccgagctt
     9901 gggcagagac gatccgccca caacaacgat gttttcagcg accccgcttt gcacgagcgc
     9961 ggcggcggta accatcgccg gaatcggcgc ggcgcaaaaa tctttgatat cgaaacccga
    10021 ggccatgtcg cagccggaaa attcggcaat cgctttggct agattaccgc cgccgcgctg
    10081 ataacggtcg ccgacggcct cctccgaaca gcccaaaaca aaatcgacat cttgcttctt
    10141 gaggctcggg tttccaccga agatttcctg aagcgacaaa gccgccgata ccttgccgca
    10201 gaggttctcc aacaatactg tcgccgatag cgactcatct tcgtgatgcc cgtcggcgat
    10261 aaatccgacc ggttcaaagt cgctgcccgc aaacagcggc aggccgccgt cttgcgcgat
    10321 cacggttttc aatttggttg aatcaacgac attgggcgta gcgcttacta gagacctgca
    10381 aatgggaagc tgccgcaaag gctcgacggt ttcctcccag aaatggtcga gcaaggcaac
    10441 atgcttgaac ggatccgcca gcgcaatcag ggcgagaaac aatttttcat cgacgatgct
    10501 gccaaaagat tttcgttcct cactgagtgc cgcaaattga tcgaaccagg gccggctgac
    10561 cgaagataat ttctcaggcg cttgctgacc gatgaaaacc tgattgggca agtaagcctg
    10621 cgcttgttga aaggatctaa gattccggtt caagtctgcg agacgcgccg gattggcgga
    10681 aatttcccgg tgcggttttg aaccataacg agtcagaccc ggggtgtgga tcaggacgct
    10741 ggaggcggct ttgattacgg gtacaaaatt agacatggtg ctcttagggt gtatcgacta
    10801 tgaccctgcc gatcaagagg cgagcctcga taagaaacga taacagacca tgactgccgt
    10861 cacgaatgat tgttccgggc tatcagcgtg gtgaaaaatg tgtttggcag gctgctcaaa
    10921 aataccgtag agcgcaacaa cgcctataag tctttttccg cagcttgcta gtaggcattg
    10981 cagatccagg actccttgcc ccaattatcg acgccgccga ttatcatatc tcgcgcgtca
    11041 aaggttagct tgccctgagc ctgcgccacg ggcgcgcccg gataactgag aatttgaacc
    11101 tgatcgtatg ggccgaccag tcgttccgct tcgggcagct ccaaccagcg gtcgcggctc
    11161 ccggtgctta ccacggcatc ggcttccgga acatagaaca acagcggcga atcgacgccg
    11221 tccttgcccc catactcata gcttaccagc actgtggcaa tgccgcgtcg ctcgcacgct
    11281 cgcacggtca gcatcacgtc gacgaaggcg ttaccggaac ctagccaggt tacgaccgcc
    11341 ccctgcgcgc cgagggtcac cgccagttgt gcggcatttt gcgcaatcac ttccttgcca
    11401 tgaaaagtct cataacggat gcgctgcaag atcatcccgc cgaaattcaa gcgccgcccg
    11461 tgatcacggt aaagcgcgaa catcagagga tggttttgcc agtcccaggt aatcggaaag
    11521 taaccgatgc cccgggtggc attgactgcc atggcgcagt caagcaactc gttgggatga
    11581 accacggtcg agagcgattc gcgtattggc aacccgtaat agctcacgcc cgagtgaaca
    11641 tgatgatcgt cggtcaaaca cccttgaact aagacgatgt tgggcaaatc cggatgcccc
    11701 tggctgagat cgaaaacctc taggttcgtg gcatcgctgc cgatggtcgc ctcggcgaga
    11761 cggcgggcaa cttggaattc tgccttttga atcgccgtgt gcgcatcgag ctccgaaagc
    11821 ccatcgcgca gcttcatgat caacaccaga tgcaaaaggc cgccgaattt tgacgccgcg
    11881 gcgcctgccc cccaggtatc gagcagagcg ctgcgctgca cgcccagtcc ggctctaacg
    11941 gtcccctcgt acgccgcagt ggcgatcacg gtcatgccgg ataagcgatg agtgcggccg
    12001 gagccgacgg cttgcaccgg tccgagtatc cccggaaaca cctgaccatt gccgcttgat
    12061 ttcacccggg gctcaacgat gtcgcggata ccggtgatgc gcaccttgtc gccgggacaa
    12121 accacagcca aggatgcctc cgcgattcgc cgatcaccca ggatcgtttc ccgcaacgcc
    12181 ggttgatcga cctccagcct tccggctcgg tattcgaact tccggcctag acgaatctcg
    12241 gtcaccggat aatcagccag ttcgagccgc atgcttgctt acccgctcac cgccatatcc
    12301 ggagtaaata ccgtcggtcc cgtgacttcg gcttcgagcg ctttcaaggc ggtctgaacg
    12361 atctctcggc gcaggcgcgc atcgagatcc tgtggcaggt tcggatcgcc gcaagggtga
    12421 ggtatgctga cgcctttaac gatgcgattg gctcgcacct gctcggccac cggatacatc
    12481 gcgctgacca gggctacggg aagtcccgcc ttctcgagtt ctttgctcat cacagcgccg
    12541 ctgcgactac aagtccctca cgtggcgacg aagagcacgc cctcgacgtt gtcctttttg
    12601 agatcggccg cgatttcttt gccgacgcgg cgcatcaccg ccggggagcc ttgattgccc
    12661 gggataacgt aataggccgg gtacagtttg ccggcgccga tcaccccttc ggcttccaac
    12721 gacctgagcg cgtccagcgg cacgccatag tgcggatttt tgttcatgta ggcgacatta
    12781 tagccgccgt gcaccgcttc ccattttccc ggttcgagct ctttgagctc ggcaatgttg
    12841 tatttacgcc aatgggtgtt gcggtaggtt ttgaatcgat ccggattgcc ccagggcacg
    12901 acaccgctgg tggtgatgac ggcgatgttg cgggctttta aatcatcgag aggcggagcc
    12961 ggcgcgatat cgtcccactc ttccatcggt atttcagtag tgaacggctc ttggttgatc
    13021 tttttgagca acatatcgat ggcgcgatcc gcgccggtcc gatcggtttt ctccaagcgg
    13081 cgcattccgc ggctgaagta accttcctgg agcgccggtc cgatggtctc ggccccggtt
    13141 agccgggaaa cgaatttcgc caacgcccta agcgcatcgg tcatgccggc ggctgtctcc
    13201 gtcgtcggta aacagaaaac ccgggcatgg gccatatcgc ggtaagtgcc gatagccgga
    13261 ttttcttcgt gcatggccgt gacgcaggga atgtccaagg ccgcggcaac ggcgtgacaa
    13321 atctcgacgc aactcatacc gtagcgcccg gaattaaacg ccggaccggc gactaatgct
    13381 tgcgcatcgt gagagcgaac cgcttcgagg atggcattga tggcttcttc gttgtgctca
    13441 tggaaatagt tatcgccgta ataaattgtg caagcgacct taatctcagc gccgagttgc
    13501 gactggagcc cacgcgccgc gccggcggtg tcttcgatca cgccgacgga catgcccgcc
    13561 ttttcttcgc ctcccaaacc ggcaaagaac tggttaacga catggacaat tgtggtcatc
    13621 gtgaaatcct cctttgcggt aatgcattca ataagcacac tatcatctct ggcatataca
    13681 tcgatagctc gccgtggcga cgacctatta gaagaacaga cactcatgac tggcccagtg
    13741 cgcgctaagc ggcaggtctg taggccgtaa atccggaacc gtccagattg
//
LOCUS       DASUVS010000320         6335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1879923, whole genome shotgun sequence.
ACCESSION   DASUVS010000320 DASUVS010000000
VERSION     DASUVS010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879923"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VF182_17135"
     CDS             complement(<1..398)
                     /locus_tag="VF182_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX6438865.1"
                     /translation="MKSKLVSLISAACALTCALSLAHADAPRSPTPAPVKGVLGVQTI
                     AKGLEHPWSLALLPDGRMLVTERPGRLRIVSADGRISEPLTGVPRVYASGQGGLLDVA
                     LSPAFDKDRLIYLSFAESGEGGAGSAVARGR"
     gene            complement(505..1203)
                     /locus_tag="VF182_17140"
     CDS             complement(505..1203)
                     /locus_tag="VF182_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX6438866.1"
                     /translation="MITVRRGNQRGSAKFSWLDSRHTFSFGDYYDPKHMGFSDLRVIN
                     EDRVDPGAGFPTHSHRDMEIITYVLEGALAHKDSTGTHSAIRVGDVQRMSAGTGISHS
                     EYNASKTEPVHFLQIWIIPNEAGLKPGYEQRSFDPKAKSGSWVLVAAPDARDESVKIH
                     QDAALYLADLSNGQTLSHRLEPGRQAWLQVTRGAATLNGTPLAAGDGAAVSKEALLQI
                     ESPSGSEILLFDLR"
     gene            2079..4865
                     /locus_tag="VF182_17145"
     CDS             2079..4865
                     /locus_tag="VF182_17145"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX6438867.1"
                     /translation="MNPLFLRNVLSRSFDIRPGDARPVVIMASLFFFLLAAINVIKVV
                     RDSLFLSRFPITHLPYVYLLAAVFAGVIIAAYNHWTEGVSVYRLILSSTAVLIFNVAL
                     FWTFIVIFELLWAIYVFYVWSAIVNLLAVAQFWTFVGTIFNPREAKRLFGIIAAGGSV
                     GAAAGGLVSGWAVNLFTGTGQLFWLIAALFAGAFGVLWLSGKELGSSRIEAPERSRFS
                     RPNPPPQPGTPRLMGGSRYLQTIAGVLFISVIVSTLIDFEFKAAAKIAYPTEEGLSAF
                     FGSYYAWMAVITFLSQVLVTGQILTIGGLIPSLALLPAGLFAGCVGMLIWPGLSSATI
                     ARVTDGALRSSVNQSGMEVLYLPLPASVRARVKSFLDVVVQRLGDGVAGLIVLFYALF
                     VMKSDPAVLIYFSLALILIWAMFIFMLRSGYLAALRTGLETRTISWNGYEVDYADKRT
                     VETVLQTLQQQDEQTVLFGLELADKLDPERVIPRLPSNLLEHPSPLVRNSSLRIFAKN
                     ATPEQMKQIVPLLQDESRYVQAEAISVVCAVRKEDAIPLMRPYLESGDPKVQRSAIAC
                     LLHHGDAEIRDLAMSAFHEMIASSGSDGVESRIESARLIGELDDPEFTADLRKLIEED
                     PSVRVVREALLAAGKRKEPTVLKEVIAKLRSAQTKNCARDALIEYGDQAVEALGSALL
                     DGDLPHAIRLSIPETLSRIGSQSALTALLGGLNQKDGSVRYRVIVALEEIARRLPNLI
                     VDRRVIETALFAEATHYYRRFLVLFTLFGDGNDLSMNGRSLLHQALLENMEREKERVL
                     RLLSLLFPDEDMHSAAAALRSGSRAAQARAIEFLDNLLTGEIKRHVFPLFDDARGLDQ
                     FEKFLALLGGRRFDAETALRELLQQDDVWLKAATLWEIGLRGLQSFRESVAHFLNSKN
                     PVIKETAELVMSRI"
     gene            4867..5304
                     /locus_tag="VF182_17150"
     CDS             4867..5304
                     /locus_tag="VF182_17150"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6438868.1"
                     /translation="MPSKNNLSTIEKLIFLKSVDIFEHATVEQLGRIAGLTEEVHFAP
                     GETIYQEGELGDAFYLLLSGRVFLHREGNTLVELGEKETFGSLDVLDFNPRAVTAKAV
                     DHVRALKLNGQELHDLLALDIEMVEAVLRMLCGLVRRILKLVP"
     gene            complement(5744..5830)
                     /locus_tag="VF182_17155"
                     /pseudo
     CDS             complement(5744..5830)
                     /locus_tag="VF182_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008211228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            complement(5861..6307)
                     /gene="trxC"
                     /locus_tag="VF182_17160"
     CDS             complement(5861..6307)
                     /gene="trxC"
                     /locus_tag="VF182_17160"
                     /inference="COORDINATES: protein motif:HMM:NF008229.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HEX6438869.1"
                     /translation="MSDPQLIRCPSCGATNRVPLEKIAQRLKPICGRCKTPLFAEAKP
                     VTITDATFAAEVERSPMPVLLDMWAPWCGPCHMVAPVIEELAAEMAGRVRVAKLNVDE
                     NPATAARFRVQSIPTLLVLREGREIDRIVGLQPKAAIVERLQRAAA"
BASE COUNT         1287 a         1785 c         1787 g         1476 t
ORIGIN      
        1 cgtccgcggg caacggcact gcctgcgccg ccttcgccgg attcggcaaa agataaatag
       61 atcaaacgat ctttatcgaa agcgggactc agggcgacgt cgagaagccc gccctgcccg
      121 ctggcataaa cccgcgggac tccggtgagc ggctcggaga tgcgcccgtc ggcactcaca
      181 attcgcaagc gaccgggacg ttcggtaacc agcatccgcc cgtccggtag gagagcgagg
      241 ctccacgggt gctcgagccc cttcgcgatt gtttgaacgc cgaggacccc tttgacgggc
      301 gccggtgtcg gcgagcgcgg cgcgtcggcg tgtgccagcg acagcgcaca agttagcgcg
      361 caggctgccg agatcagaga cactaacttc gacttcatga tgaacacttt cgtcgaccgg
      421 ggctgaataa gaaacccgga cgcgcggctg gaacaaacca gcattgaagc ttcacacttc
      481 cagcttcagt tcgtgatcca ggccttatcg cagatcgaac agcaggatct cactgcccga
      541 cggcgattcg atctgcagga gcgcttcttt gctgacggct gcgccatctc ctgcagccag
      601 cggagttccg ttgagcgtcg cagcgcctcg cgtcacttgt aaccacgcct gccggcctgg
      661 ttctaaccga tggctcaatg tttggccatt ggaaaggtcg gctagataga gcgccgcgtc
      721 ctgatggatc tttacagatt cgtcacgcgc gtccggcgcg gcgacgagaa cccagcttcc
      781 agactttgcc ttaggatcga aggagcgctg ttcgtatccc ggcttcaaac cagcttcgtt
      841 tggaatgatc cagatttgca ggaaatgcac tggttctgtt ttagacgcgt tgtactcgct
      901 atggctgatg ccggtcccgg cgctcatgcg ctgcacgtcg cccactcgga tggctgaatg
      961 cgttcccgtg ctgtctttgt gcgccagcgc gccttccaac acataagtga tgatctccat
     1021 atcgcggtga gagtgggtcg ggaaaccggc tccggggtcg acccggtcct cgttaatgac
     1081 gcggagatcg ctaaagccca tatgtttagg atcgtaatag tcgccgaatg agaacgtgtg
     1141 gcggctatcg agccagctga acttcgcgga ccctctttgg ttaccccggc gtaccgtgat
     1201 catgatccct ccacgtcatc atttatagag atacaaactc tagcagctac ttgcgtgcga
     1261 caacagcggc gaggatgacg tattccggat agacaaatgc cgccgctcca agcgccaacg
     1321 tgtacggcat actcaacgcc cattccgtag gcgccccaat aatgcttcac gatatccatg
     1381 atcgcgttaa cggtgccgcg ctcgcccgcc attttcactt ggctttggtg gagcgttttt
     1441 tgagccgtaa cttcctcctg tctgaaattg ttgatggaca ctatcgattt cgctgtatag
     1501 tacccgaagg cgtgaaagat cttggctttg tggtttagct cccgattctg ggtatagttc
     1561 cacctaaaga taaggccaca gcgacgcgca cgtttgacat cctgtcggcg gattcgcgcc
     1621 cacggatgac gtggagctgc tgtcgtgccg agcgggcggc ggactatcct aggcgcaaca
     1681 catgtaggtt gttttggata tgccgccgga tacgataact atttagcaga tcgtattccg
     1741 ggcccgcacg tcccgacgaa gaaaagcagc gcaatggatg tgccactttg gcacgattct
     1801 tcacccgccg gccaaatagc cattgtcgca caatagcgaa tcgcgctgca tcgcatgaag
     1861 caggagtgcg acaccacgaa tgcgagaggt taatggccgt ggtcgcgcta tcttggccgc
     1921 aaaagagctg gaccagtaga agaagctttg ccaatattag gacgttgaac atcgaggata
     1981 tttacaggcc tacgcggata acccgccgtg ttcgcatcgt agtactcggg tcgtctttgg
     2041 gcggagtttg aagtactctc ggcatttctt tagctcggat gaatccgctt tttcttagaa
     2101 acgttctgtc gcgctccttc gatatcagac ccggtgatgc ccgccctgtg gtcatcatgg
     2161 catcgctttt tttcttcctt ctcgccgcca tcaacgtcat caaagtagtc agagactctc
     2221 tttttctaag ccgctttccc atcacgcacc tgccctatgt ttatctcttg gccgcagttt
     2281 ttgcgggggt catcattgct gcttacaacc attggacgga aggggtttca gtttaccggc
     2341 tcatcctgag ctccactgcg gttctcattt tcaacgtagc tttattctgg acgtttatag
     2401 tcatctttga gcttctctgg gcgatatacg tgttttacgt ctggtccgcc attgtcaatc
     2461 ttctcgccgt tgctcaattc tggaccttcg tcggcacaat cttcaatccg cgtgaggcga
     2521 aacgtctgtt cgggattatc gcggccggtg gctcggtcgg cgcagccgcc ggtgggctcg
     2581 tcagcggctg ggcggttaat ctgttcaccg gaaccggcca actcttctgg ttgatcgcag
     2641 ctctgttcgc aggcgctttc ggagtgcttt ggctgtccgg gaaggaactc ggctcgtcgc
     2701 gcatcgaagc gccggagaga agccggttct ccagaccaaa cccgccgccg caacccggaa
     2761 cgcccaggtt gatgggaggc tcccgttatc tccagacgat cgcgggcgtt ctcttcatct
     2821 ccgtgatcgt atcgactctg atcgactttg aattcaaagc cgccgccaag atcgcctatc
     2881 cgacggagga aggtctcagc gccttcttcg gaagctatta tgcctggatg gcggtcatca
     2941 cgttcttgtc tcaggtgctc gtcaccggac agattctaac catcggcggt ctgattccga
     3001 gtctggcgct cctgcctgcc ggcctgtttg ccgggtgtgt agggatgttg atctggcccg
     3061 ggctgtcgtc ggcgaccatc gcgcgagtca cagacggggc cttacgatcg agtgtcaatc
     3121 agagcggcat ggaggtcctc tatcttcctc tcccggcgag cgttagagca cgggtcaaga
     3181 gctttctcga cgttgtcgtg cagcggcttg gagatggcgt cgccggcctg atcgtgctgt
     3241 tttatgcttt atttgtgatg aagtccgatc cggctgttct catctatttt tctctcgctc
     3301 tcatccttat ctgggcgatg ttcattttca tgctgcgcag cggctacctt gccgcactac
     3361 gaactgggtt ggaaaccagg acgattagct ggaacggata cgaagttgat tatgctgata
     3421 agcgcaccgt tgaaaccgtc ctgcaaacgc tgcaacaaca agacgagcag acggtattat
     3481 tcggcctcga gctggcagac aagctggatc cggaacgcgt cattcctcgc cttccgtcca
     3541 atctcctcga acatccttct ccgctggtcc ggaatagttc gttgagaatt ttcgccaaaa
     3601 acgccactcc cgaacaaatg aagcagatag tgccgctgct acaggatgag agtcggtatg
     3661 tccaggcaga agcgatcagc gtggtctgcg ccgtgcgcaa ggaagatgcg atcccgctca
     3721 tgcgccctta cctggagagc ggtgatccaa aagttcagag atcagcgatt gcctgcctgc
     3781 tgcaccacgg ggacgccgaa atccgtgacc ttgccatgag cgcgttccac gaaatgatcg
     3841 caagctcagg aagcgatgga gtagaaagcc gaatagagtc tgcccgtctg atcggcgagc
     3901 tagacgaccc ggagtttaca gcggacctga gaaaattgat cgaggaagat ccttctgttc
     3961 gcgtcgtgcg tgaggcgctg cttgccgccg gcaaacgaaa ggagcccacg gttctgaaag
     4021 aggttatagc aaagctccgt tccgcccaaa caaagaactg cgcgcgtgat gcattgatcg
     4081 agtatggcga tcaagccgtg gaagcgctgg gaagtgccct cctcgacggc gatctccctc
     4141 atgcgatccg gctaagcatt cccgagacac taagcaggat cggctcccaa agcgcgctaa
     4201 cggcgctact cggcggcttg aaccagaaag acggctcggt tcgctacaga gtgatcgttg
     4261 cgctcgaaga gatcgcgcga cggctgccca atttaatagt agaccggcgg gtcatcgaga
     4321 ccgccctttt cgccgaagcc actcactatt accggcgttt cctggttctc ttcacccttt
     4381 tcggagacgg caacgacctg tcaatgaacg ggcggtcatt gctgcatcaa gcattgctcg
     4441 agaatatgga aagggaaaaa gaacgggtgc tccggctgct atcactgctc tttcccgacg
     4501 aagatatgca cagcgctgcc gccgcgctgc gtagcggcag ccgcgccgcg caggctcgcg
     4561 ccatcgagtt tctcgacaat ctgctcaccg gagagataaa gcggcacgtt ttcccgctgt
     4621 ttgatgatgc ccgcggcctc gatcagttcg agaagtttct tgcgttattg ggggggagac
     4681 gctttgatgc agaaacggca cttcgggagc tgttgcagca ggatgacgtc tggttgaaag
     4741 cggccactct gtgggagata ggattgagag ggctgcaaag cttccgagaa agcgtcgcgc
     4801 actttttgaa ttccaagaac cccgtgatca aggaaaccgc cgagctcgtg atgtcgagga
     4861 tctagcttgc catccaaaaa caatttatcg acgattgaaa aacttatctt cctcaaatcc
     4921 gtagacattt ttgagcatgc caccgtagag caactgggtc ggatcgccgg attaaccgaa
     4981 gaggttcatt tcgcgccggg agaaaccatc taccaagagg gagagcttgg cgacgctttc
     5041 tacctcctct taagcggtcg ggtgtttctt caccgcgaag gcaatactct tgttgagttg
     5101 ggagaaaaag aaacctttgg aagcctcgac gttctggatt tcaacccgcg ggcggtcacc
     5161 gcaaaggccg tcgatcacgt ccgtgcactg aagcttaacg gccaggagct tcatgatctt
     5221 ttagcgctgg atatcgaaat ggtcgaggcc gtcttgcgga tgttgtgtgg cctggttcgc
     5281 cggattttaa agttggtccc gtaagtgttg cgcggcctgc ttcccgctcc agatggagca
     5341 tgagatcttc gagcacgccg tcgttatcga gcagtgtatt atccacgtcg agcggaaaaa
     5401 ctacctgttt agcaagcata cgttggactt ccttcccgtc gatcctttgc tgtcatatgc
     5461 tttgcgagcg ctcgccgtcc gaggcttttg cttgcgccct ctgttcatcg gcacggtggg
     5521 ctgctttttt tcttcggctc gagaacttgt cgcacaaggg ccacctgcgc cagtttattt
     5581 gtcatatgcg agtgcgatct gcatagacag cctaaaaatg agttgcgcga ttgcggaacg
     5641 tccagggatt caatggtagc gccgacagta gcgcgccgtt ccctcgtggc tttagattgc
     5701 cagggcgagt tgcgatcgtg cattgtcatc ttaggacgga tttctagttt gcaagctgcc
     5761 ggatcacgcg acaggggttg ccaacggcga gcattgccgc gggaagatcc cgtgtcacta
     5821 cactgccgct gctgctacgc gaggcgcttc tcgtgagaac tcaggcagcc gctctttgga
     5881 gacgctctac gatcgccgct ttgggctgca agccgacgat acggtcgatt tcgcgccctt
     5941 ctcggagcac caagagagtg ggaatgctct ggacacggaa ccgcgccgcc gttgccggat
     6001 tctcgtcgac attaagtttc gccactcgca cgcgacccgc catttccgcg gccaactctt
     6061 caatcaccgg cgctaccatg tggcagggtc cgcaccatgg cgcccacata tcgagcagca
     6121 ccggcatagg ggagcgctcg acttcggccg caaaggtggc gtccgtgatc gtcaccggtt
     6181 tggcctccgc gaacaaaggt gttttgcacc ggccgcatat gggtttaagc cgctgagcga
     6241 ttttttccag cgggactcgg ttagtcgcgc cacaggaggg acaacgaatg agttgcggat
     6301 cagacatatg ttcgaccttt catatctggc ttgtg
//
LOCUS       DASUVS010000321        16634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1664413, whole genome shotgun sequence.
ACCESSION   DASUVS010000321 DASUVS010000000
VERSION     DASUVS010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 16634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16634
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664413"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1360..2457
                     /locus_tag="VF182_17165"
     CDS             1360..2457
                     /locus_tag="VF182_17165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6438870.1"
                     /translation="MAILKIERLRKRFNEVQALEDVSFEFDHGILSFLGPSGCGKTTL
                     LRSIAGLEIPDAGSIFIADQVQTSIERGILVPPYAREIGFVFQNYALWPHMTVFKNVA
                     FGLKLRKRPAEEVRRKVLNALDLVGLQGREERYPSQLSGGQQQRVALARSLALEPRLI
                     LLDEPLSNLDAKLREEMRSELKKLIKKVGISALYVTHDQEEAFTISDSVIVMENGRIL
                     QYATPNEIYNRPAHAFVASFIGHSTLLEGKIVRVDGAECLVAIPEFDGATLLSPVSTG
                     AVPGRSCKVVIRTSEIRLSHESFAESADNVLEGNMTGRDFRGGLTDHHIRVGSRELVV
                     TSHKLCPMISIDGESGKTYLSIDRSAISLIL"
     gene            2566..4212
                     /locus_tag="VF182_17170"
     CDS             2566..4212
                     /locus_tag="VF182_17170"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6438871.1"
                     /translation="MIASLIAGAAIITPVIIVMLRSLTTGKLGVAMGFSAENYLRVFS
                     DRDIWSMLNNSIIYAAGSAALGTGLGAMLAWIVARTNTPGKALVELMPLYPILMPPIM
                     KNIAWILLLAPKSGILNNMLQQYFGATGLFFNAFSMAGMVWVFGLACVPLGYLFLLPV
                     FLSFDPSLEESAYIAGSKPVQTMLRITFPLALPAFLSALVLNFLRGLRSFETPVLQGT
                     PAGIKVFVARVYDSMALEFNTGLATSYSMVLVALSVITLIIYVRTTRFSERYATITGK
                     GYRVKVIDIGPWKYVTFLAVLFYFLVGIAIPFSVLIVVSMIPYFDYETFMQFPTNAVL
                     TNYYTVLKHPSFVTGLYNSLTLSITIALVTVLAGIIMAFTIYRTRAHGAKAFEFIGTL
                     PLAFPPLVLSVGLVIIFLGTPLYNSLWALGLGLFVAYFPYAFRNASGSIVNIHKELDE
                     AAWVHGARWRHVFFKITLPILKPSVGGALFYIFIEAVRNVDVAVLLTSPGKEYGPVTL
                     FEYFRVGQWAEAAAGGVIYLVILIIAVSIAKFAFKMKFSL"
     gene            4247..5395
                     /locus_tag="VF182_17175"
     CDS             4247..5395
                     /locus_tag="VF182_17175"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6438872.1"
                     /translation="MIKNYLLATAVALSAALYLPVSGQAADAPSKGGGDAKSSGQKPG
                     QQRVEKAAPNATANALKAFEKVSQELYPKAKQEGNLVVYSVWDVEHIRAVTDAFAKRY
                     PGIKTAYWQGRNPEIVTRVLTEFQGGQASVDVVLSDNAPPVLRAAGAMASYETVQKEV
                     LYLHDPTLPTVSLQIQALAYNTKKIKPNDLPKSWEDIANPKYKGMIALDDPMRAGPLS
                     SMLAGLKTQWNDDARFNKFLKGLKALNVPVHKSTSAMFRLVISGEYPICMPALLHDVI
                     HEKEKGVPVDYVKTAPPVVFPRQAGIYAKAPNPNAARLFAEWLISDEGQKTIDAVGRE
                     TSRNDFKSKTSIETAFPKGTKPLPVIDRAFLEDPKKWLDTNVKPIWEG"
     gene            5704..5794
                     /locus_tag="VF182_17180"
     tRNA            5704..5794
                     /locus_tag="VF182_17180"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5738..5740,aa:Ser,seq:gct)
     gene            6461..6877
                     /locus_tag="VF182_17185"
     CDS             6461..6877
                     /locus_tag="VF182_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6438873.1"
                     /translation="METETILRVARPTNHLDQIVRFYIDGVGLIRLGAFEDHDGFDGV
                     MLGLAGAPYHFEFTRKRGHLAGAAPTQDNLLVFYLPEIEQWQAAVDRMVAAGYEPVPS
                     FNPYWDRAGRTFEDPDGYRIVFQNAHWPGQSSTDGE"
     gene            complement(6892..7665)
                     /locus_tag="VF182_17190"
     CDS             complement(6892..7665)
                     /locus_tag="VF182_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX6438874.1"
                     /translation="MDCTHARRLLDTYLDGELDPVRSLEIDEHLHGCADCSQSYGEGQ
                     SFHKALKSGSLYFKAPADLHKRIQRSVREAARTETPSGWFSPSWIKTAVPLAAAALVL
                     VLFIPTLKGPSSDELLTQDIVASHIRSLMADHLADVPSSDEHTVKPWFNGKLDFSPPV
                     SDLAKEGFPLVGGRLDYLNNRPVAALVYRRDAHVINVFLWPSKTTAQASAPIKMQQGY
                     RVIRWTGNGMQFSVVSDLEASQLVKFAHLLKSSAGTPAR"
     gene            complement(7665..8192)
                     /locus_tag="VF182_17195"
     CDS             complement(7665..8192)
                     /locus_tag="VF182_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6438875.1"
                     /translation="MRGNEKLQRFEQAIMPHLDAAYNLARWLMRNEDDARDMVQEAYL
                     RAFKFFDGFHGVDARAWLLTTVRNTCYTWLEQNRRSRATTSFDEEIHSLDEQATNPSE
                     LALKSGDVEMIRESLEELPEEFREVIVLRDLEEMSYKRIAEVINIPLGTVMSRLARAR
                     GRLRRILFSRLKEIS"
     gene            9424..10449
                     /locus_tag="VF182_17200"
     CDS             9424..10449
                     /locus_tag="VF182_17200"
                     /inference="COORDINATES: protein motif:HMM:NF014312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HEX6438876.1"
                     /translation="MRRKKQMKLMLSGFGYLDVLIAGVLLLGASSQIVEPASAAPAAK
                     AYVANFTDNTVSVIDTESAKVIATIPVAAGPHGMAITGDGRTLYVSGDRSSSLNVIDT
                     AVDRVVKTIDIGKTPNGVALTPDNRLLLVTIYDEDRVVFVDPATQSAIAAVPVSKPHT
                     VSISPDGKFAYVTVQEPGHFGLARIDLNSRSLVHTLPLDKTPRDGEFSFDGKMFYFTQ
                     AGVNSVQVLDPASEKIVAQIPTGVSPHFVGHPVGSAFGIVVVQGPGELSLFDPATHKP
                     TRNVSVGKQPHWAATSQDGKTTYVTDEGSNDVTVVEMATGATKTIAVGNAPRKVVIQP
                     MAARKES"
     gene            10451..10906
                     /locus_tag="VF182_17205"
     CDS             10451..10906
                     /locus_tag="VF182_17205"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX6438877.1"
                     /translation="MRNHNHSIDNLHTLFTAVMSALSSGLFAFVVLTQPAQQIFAAPA
                     GDLGQVHSSANTFAQRAATAPVENGAKVSIVNFAFTPEQITINPGETITWINDDGAPH
                     ALKFQDGAKGTDLMLPGATFRRHFDGPGTYDYSCSVHPYMTGRVVVTIR"
     gene            12011..12628
                     /locus_tag="VF182_17210"
     CDS             12011..12628
                     /locus_tag="VF182_17210"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX6438878.1"
                     /translation="MPRANGGRTLFDAPAQVPDGFIYNEDFISETEEQELIHEIRNLP
                     LEPFKYYRFTGKRRTVSFGWQYEFGASEITEAPEMPNFLLPLRTRAGNVFNIDPNSLV
                     QTSIIEYSTGSPIGWHRDIPQFGIVIGVSLGAACRMRFRKFRRVRSKKLDRDQVLSIE
                     LHPRSIYLMSGASREVWQHSIPALKALRYAIMMRTLRVKSERARA"
     gene            12848..13633
                     /locus_tag="VF182_17215"
     CDS             12848..13633
                     /locus_tag="VF182_17215"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX6438879.1"
                     /translation="MQHLAKIPFPEVERLSQAGKALVILPVGVVEEHGAHLPLGVDSF
                     AAEAYAIAAAPHLEEKGYEVILAPTIDYGVARQALNFAGTLTLQPKTLESLVVDIGRS
                     LAKHRFRRLVILNGHRDLGHMKTLEAARSVLVAEETAQVLCTGFTSDPVVTAACYREG
                     VKQFYRSPRPDREGHGGESETSVGLYCFPELVRQELIGQLEPNFDYDVEAFHNETEDY
                     GILSDGRGYFGSPQAASAETGKQLVLTRGRNIAQVILNAWGAL"
     gene            complement(13812..15203)
                     /locus_tag="VF182_17220"
     CDS             complement(13812..15203)
                     /locus_tag="VF182_17220"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX6438880.1"
                     /translation="MRSLPGIFVYSWLAAALLGCSLSHPLPIDPTGRDEAGLHSSMAI
                     NTAPVTESQTASAPAIRAQKKDLPSNAEVSSASLSQKTVEKTASLEQQHKTPADTRTI
                     DELPNAASDDDLIELWRRDMDRAMQEPAEQRILLISPAVEAHDRVRFYVEGFCGRMRD
                     FFERALARSGKYRSMMAEILRSEGLPDKLVYLALIESGFSTHAYSRAQALGPWQFMRG
                     TALQYGLTINSWLDERRDPVLSTRAAAAYLKDLHERFGEWFLAAAAYNAGEGRVGTAL
                     QRSNTNDYWLLRQERKHLKLETRDYVPKFIAAALIARQPERYGFEEISYESPMEYDEV
                     TINHPLRLETVARLANTDVDVIKELNPALLQNFTPPTDGAFTLRLPVGSQADFLAGYE
                     QLTGPDKIKLSLHRVKKGELLARIAKKYNVTPKQLVKENRLKSWHLRPGQELIIVSND
                     AGAPLAANAPRLP"
     gene            complement(15623..16531)
                     /locus_tag="VF182_17225"
     CDS             complement(15623..16531)
                     /locus_tag="VF182_17225"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6438881.1"
                     /translation="MGYLPKKGVSFELIEFQSAGRTVQGGLFGPDPELFHEAGTAVVL
                     VHGVEAYWYSGPTMFLGCYLAEAGYAALAYNGVHSGESFRTSEFEAAVKEVEDAITFA
                     NGRGFKNIFLAGHSLGTPIIEYYQGNKPHPSVKALGVYGPHIDIPSVTRESLLGSELY
                     ENFAAECRTLVSSGKGEEIKLLPFREGKVIITSAKTFLSYRDVETSKAAVQKMIANIR
                     VPLLIVYDRADNIQGKGSITRRETIAARIKESAVNCPRADIVVIPSHPGNSSFQAHLF
                     VNNERMVTEETINWLTNIGLAPASVR"
BASE COUNT         3854 a         4320 c         4502 g         3958 t
ORIGIN      
        1 aaatccaaag taatcaaggt tccggggtaa tcgtggaccg tgttgacaat gcgcgattga
       61 catcgtttat ctgaattcaa ctgctcatcc ctgactataa cgggctacat gcgaggcttt
      121 gataccgcca ttcgagacta agcggctgcc ctcatggtgt ggcgtgtacc tgctgcactc
      181 ccccaccgct atcggtaaat aactgtgtgc gttgaatcag agccgacagc atcgcaagtg
      241 gtttccaagt tcgctagccg atttgtgggg gctgacgcta cgactgctcc gcctaagcct
      301 tattcgagaa ctgcggcttt actggatgaa cgaaaatgac gcctgaacgc gttaaggttt
      361 cgccattcat gaaccatgag aacaaaaccc aatccggcac atacaattcc aagaactgcc
      421 aacatcaagt cgatgaattc catgtcttca atgtaccggc cttcacatag aattcaatag
      481 gctctataga atttagcaat acgtcttctc aggaatatgc cgttacagaa aaagccatag
      541 ggccaatgtt gctaatactc cacaaaacat cgcgatcttt agaatcgagt ctacgaacat
      601 gtcgcacctc gcctgttgaa attccgtgaa ttacctcgat gagtcattac ctttaatgag
      661 taaaagtggt ttgtcgaagg cctcccgcta cgtgcagagc gatttctgag atgccgcgtt
      721 agaccgcggc gttgtcctca agaaagcgtc gggaggatcg cacgccgacg gagttgtcga
      781 actcgtgacg tagtctcctt ggaacgcggt gattaagaat gcagccagca gcagatacag
      841 gcccagaaaa aacagcatca aaacggacac ggggaataga tcttccctgc acttcggcaa
      901 aaattttggc attctgatca ggggagtacc tccgcaagga aaaatttcga gccaaaagcg
      961 gccggttatg tatcacagca gtcgtttttg gcaaggggca ggggagcagc agtgccagtg
     1021 gctgtcagct tgaagttaaa agagtattgt ttcgatgttg tcgtatgcgg ctttaacact
     1081 ggtttcgttg gttgctgcac gatttggttt ccattttcac cgcctttaga gtagaagaat
     1141 tcatttcgtc gggatgttta tcggcggggg gaagacgaca tttcattcta cacatggaat
     1201 tccgtgatgc acactcgagg agttaagctg tcaaaactcg ccggtcatgg cgcgatcagc
     1261 gccgagcgga atttggatct gccaattcta accagaagct ctattgaaaa gcatgcttcg
     1321 actttgacgt tccctggtcg cgctgtttcc cgctaagcca tggctatctt aaaaatcgaa
     1381 cggttgcgca aacggttcaa cgaagttcag gcgctcgagg acgtgagctt cgaatttgat
     1441 cacggcatcc tttcctttct cggaccttcg ggctgcggca aaaccacgct cctgagatct
     1501 attgccggcc tcgaaattcc cgatgcaggc tcgatcttca ttgccgatca ggttcagact
     1561 tcgatcgaac gcggtattct cgtgccgcct tatgccaggg agattggttt tgtttttcag
     1621 aattatgcgc tttggccgca catgacggtg ttcaagaatg ttgcgttcgg cctcaagctg
     1681 cgaaagcggc cggcagagga ggttcggcgc aaggttttga atgccctgga tctagtcgga
     1741 ttgcagggta gggaggagcg ctatccgtcc cagttgagcg gcgggcaaca acaacgcgtt
     1801 gcattggcgc gaagtttagc cttggagcct cgactcattt tgctcgacga accgcttagt
     1861 aatctagacg ctaaattgcg cgaagagatg cgtagcgagc ttaaaaagct aatcaagaag
     1921 gtcggtatca gcgcgcttta cgttacacac gatcaggagg aggccttcac catatcggat
     1981 tcagttatcg tcatggaaaa tggcagaatt ctccagtacg caactcccaa cgagatctac
     2041 aatcgtccgg ctcatgcctt cgtcgcctcc ttcattggcc actcgactct gctcgaaggt
     2101 aaaatagtca gggtggatgg cgcggagtgt ttagttgcga tcccggagtt tgatggggca
     2161 acattgctct ctcccgtcag caccggagcc gttccgggcc gatcttgcaa ggtcgttatc
     2221 cgcacgagcg aaatccgttt gagtcacgag tcgtttgccg agagtgccga caatgtactc
     2281 gaggggaaca tgacgggtcg ggactttcgt ggcggcctta ccgatcatca tattcgagtc
     2341 ggttcgcgcg agttggtcgt cacctctcat aagctatgcc cgatgatcag tattgacggc
     2401 gagtccggca agacctatct ctcaattgac cgctcagcga ttagtctgat cctatgacga
     2461 ttaacaggca ttcttcgggc tgctgcctgg gcccgtctcc gaggagcaac ggcgattaac
     2521 tggaattcct tcaagctggc ataccgccaa ggaacctttg ttccgatgat cgccagcctt
     2581 attgccggcg ccgcgatcat tacgccggtg ataatcgtta tgttgcgcag tctcacaacc
     2641 ggcaagctcg gtgtcgctat gggtttctcc gccgagaatt atttacgagt gttttccgac
     2701 cgggatatct ggtcgatgct gaacaattct attatctatg cggcaggatc ggcggcattg
     2761 ggaacgggtc tgggcgccat gttggcgtgg atcgtcgctc gaacgaacac gccgggtaaa
     2821 gctttggtcg agctgatgcc gctgtatccc atcttgatgc cgcccatcat gaaaaacatc
     2881 gcctggattc tgctgttggc tccgaaatcc ggcatcctaa acaatatgtt gcaacaatat
     2941 tttggcgcaa ccgggttgtt tttcaatgcc ttcagcatgg cgggtatggt gtgggtgttt
     3001 ggcttggcct gtgtgccgtt gggctacctc tttttacttc cggtgtttct gtcgttcgat
     3061 ccgtcgctgg aggaaagcgc ctatatcgcc ggcagcaagc ccgtacaaac gatgctgcgt
     3121 ataacgtttc cactggcgct gccggctttc ctctcagccc tggtgctcaa cttcttgcgt
     3181 ggcctgcgct ccttcgaaac cccggtgctg caaggaacac cggccgggat caaagttttt
     3241 gtagcccggg tgtacgactc gatggcctta gaattcaata ccggactggc gacttcttac
     3301 agcatggttc tagtggcact ttcggtgatc actctgatca tctacgtccg gaccacacgt
     3361 ttttccgagc gctacgcgac gattaccggg aagggctatc gagtcaaagt aatcgacatt
     3421 ggtccctgga aatatgttac atttctcgcc gttttattct attttctggt tggcattgcc
     3481 atcccgttta gtgttttgat cgtggtctca atgatccctt acttcgatta cgaaactttt
     3541 atgcaatttc ccacaaatgc cgtgttgacc aattactaca cggttttgaa gcatccaagc
     3601 ttcgtgaccg gtctttacaa ttctctgacg ctgtccataa ccatagctct ggtaaccgtg
     3661 ctagctggaa tcatcatggc cttcacgatc tatcgcaccc gtgcccatgg cgccaaggct
     3721 ttcgaattca tcggtacctt gccgctcgcg tttccgccac tggtgctctc agtgggactg
     3781 gtgatcattt ttctaggaac accgctttac aatagtctat gggccctggg cctggggctt
     3841 tttgtcgcct atttccctta tgcgtttcga aacgcctcgg gttccatcgt caatatccat
     3901 aaagagctcg acgaagcggc ttgggtgcat ggcgcgcgct ggcgccatgt cttcttcaag
     3961 atcacgttgc ccattttgaa accgtcggtc ggcggcgcgc tattctatat ttttatcgaa
     4021 gccgtccgta acgtcgatgt agcggtcttg ctgacatccc ctgggaaaga atatggaccg
     4081 gttactcttt ttgaatattt ccgagtaggg cagtgggccg aagccgccgc cggcggggtg
     4141 atctatctgg tgatcttgat aatagccgtg tccatcgcaa agtttgcgtt caaaatgaag
     4201 tttagtttgt aagggatcta gaaaggatca acaagggggg agcgaaatga tcaaaaacta
     4261 tttgctagca acagcggtcg cactctcagc ggcgctttat ctgcctgtgt cggggcaggc
     4321 ggccgatgcg ccttcaaaag gaggcgggga tgctaagagc agtggccaga agccgggtca
     4381 gcagagggtt gagaaagccg cgcccaacgc gaccgcaaac gcactcaaag cgtttgaaaa
     4441 ggtcagtcag gaactctatc cgaaagcgaa gcaggaagga aatttggtcg tctactcggt
     4501 gtgggatgtg gaacatatcc gtgctgtgac ggacgccttt gccaagcgat accccgggat
     4561 caaaacggct tactggcagg gccgcaatcc cgaaatcgtg acgcgagtgc tcacggaatt
     4621 tcaaggcggc caagcgagtg tcgatgtcgt gctctcggac aacgctccgc cggtgttgcg
     4681 cgccgccggc gccatggcca gctatgaaac agtccagaag gaggttcttt atcttcatga
     4741 tccgaccttg ccgacggtga gcctccaaat tcaggctctt gcgtataata ccaagaagat
     4801 caaaccgaac gatttgccga agagctggga agatattgcc aatccgaagt acaaagggat
     4861 gatcgccttg gacgacccga tgcgggccgg cccgctgagc tcgatgctgg caggtctgaa
     4921 gacgcaatgg aacgatgatg cgcgcttcaa taagtttctt aagggcctca aggcgcttaa
     4981 cgtgccggta cacaagagta ctagcgccat gttcaggctg gtgatttccg gggagtaccc
     5041 gatctgcatg ccggcgttgt tgcacgatgt tattcatgag aaagaaaaag gcgtccccgt
     5101 cgattacgtg aaaacggccc cgccggtagt ctttccgcgg caggcgggaa tttacgctaa
     5161 ggcgccgaat ccgaacgcag ccaggctttt cgcggaatgg cttatctcgg atgaggggca
     5221 gaaaaccatt gacgccgtcg gtcgagagac gtcgcgcaac gattttaaat ccaaaacttc
     5281 catcgagacc gcatttccca aaggcaccaa gccacttcca gtcatcgata gagcttttct
     5341 cgaggacccc aagaaatggc tcgatactaa tgtaaagccc atctgggaag gctgatttcc
     5401 gtcgagttgc tgatgccggc gaggaaagct ggttcgcgca cggcggatca gcttccctcg
     5461 ttgcttttcg tgctgcgatg cgcgttttgc cataaaactt cgcacccagc tgtgttgatg
     5521 atgcaaatag gtcgccgggg aaattaccta tgcatcgtaa ttcgtcgtgg gaccgagatc
     5581 gggcgtggga ttgtggaatc gtgcaaggtc ctatgttagt ttacaggctg cttcggcaca
     5641 gttcgaagca taaaagcttg agcccgcttc tggttcaacc gccatataaa tcatttcgtt
     5701 caaggagaga tggccgagtg gtctaaggcg cacgcctgct aagcgtgtgt acctcaaaag
     5761 ggtaccgagg gttcaaatcc ctctctctcc gccattttct tctgaatcta aaatcgctga
     5821 catacgcccg gattccgcgc cagaatcgtt ggttggagca cgagcctcta cattaaagct
     5881 ctatgtgata aaataccgtg caacaactgg aatctcctag aggatgacgg ccaggccgtt
     5941 tgctcgaccg gtagaacagt ggagccacga cactcaagga tctcatcgcg ggtagccaac
     6001 ttaggctttc tgtcacgggc cgaatcgcgc atgtggcgct gactacagta gggcttgccg
     6061 tattgcaatg ccgagactcc tcttgctctt aatctgagga aagctgatcg agagctatcc
     6121 tgcggcaccc atttgcttgg tatctcgagc cagaacgcag cggcaggttc aacgcgaaac
     6181 atgtcttgta tcaatcacca ttgttttgaa tagagcgggc tgcttcgtgg ctcagccaaa
     6241 ttactttctt cctgaagcgt gtgtaacgtc aaagacgaga tccctgacgt taagcccatc
     6301 cgtatagctg acgcgtgctt catctcccac ggttatcagg gtccaaggag aagaagatga
     6361 gactttccac aattcacgct gtctttttcc gattatgtcg attgttctgc aacctcttct
     6421 ctgcaagagg catctaagta agagcgttct tgtatcttgc atggaaacag aaacgatcct
     6481 acgagtcgcg cgaccgacaa atcatctcga tcaaatcgta cggttctata tcgatggagt
     6541 cggcttgatt cgattaggag cgttcgagga tcatgacggt ttcgacggcg tgatgctcgg
     6601 gttggccggc gcgccgtacc atttcgagtt tacccgaaag cggggccacc tcgcaggtgc
     6661 tgcgccgacc caagacaatt tgctggtctt ctatcttcct gagatcgagc agtggcaagc
     6721 ggcggttgat cgtatggttg cggccggata cgagcccgtt ccctcgttta atccgtattg
     6781 ggaccgggcc ggccgtactt ttgaagaccc ggacggctat cgcatcgtgt ttcaaaatgc
     6841 gcactggccg ggtcagtcct ccacagacgg tgagtaaaaa cggaacaatc attatctggc
     6901 cggagtgccg gccgatgatt ttaacaaatg ggcgaacttc acgagctggc ttgcttccag
     6961 atcggagacg accgagaatt gcattccgtt accggtccag cgaatcacgc gataaccctg
     7021 ctgcattttt atcggagcgc ttgcttgtgc ggtcgtcttc gacggccaga gaaaaacatt
     7081 gatgacatgc gcatcgcgcc tataaaccaa cgcggcaacc ggacgattat tcaaatagtc
     7141 gagacgaccg ccgaccagag gaaacccttc ctttgccaag tcgctcaccg gcggggaaaa
     7201 atcgagtttg ccgttgaacc aaggcttgac cgtatgttca tcgctcgacg gaacatcggc
     7261 caggtgatcc gccatcaacg aacggatgtg gcttgcgaca atgtcctggg tcaggagttc
     7321 atccgatgat gggcctttca gcgttggaat gaacaacacg aggaccagcg cggcggcagc
     7381 caacggcacc gccgtcttta tccatgaggg agaaaaccaa ccagacggcg tttcggtcct
     7441 ggcggcttca cgcaccgaac gctgaatgcg tttgtgcaga tcggctggag ctttgaaata
     7501 aagcgatccg ctcttgaggg ctttgtgaaa ggactgcccc tcgccgtagc tctgcgaaca
     7561 atcagcgcag ccgtgaagat gttcgtcgat ctccaggcta cgcacgggat cgagctcacc
     7621 gtccaggtaa gtatcgagca atctccgcgc gtgcgtgcag tccattagga tatctccttg
     7681 agcctcgaga acagaatccg cctgagccgg ccgcgggcgc gcgccagacg tgacatgacg
     7741 gtgccgagag ggatgttaat gacctcggca attctcttat aggacatttc ctcgagatca
     7801 cgcagcacaa tcacctcgcg gaactcttcg ggcagctcct ctagcgactc tctaatcatt
     7861 tccacatcac cgctcttgag cgccagttcg gacgggttgg tcgcctgttc gtcgagcgaa
     7921 tgaatctcct cgtcgaacga agttgtggcc cggctacgcc ggttttgctc gagccaggtg
     7981 tagcaggtgt tgcgcacggt ggtgagcagc caagcgcgcg catcgacccc gtgaaatccg
     8041 tcgaagaatt taaacgcgcg caaataggcc tcctgaacca tatctcgggc gtcatcttca
     8101 ttgcgcatca accagcgggc caaattatag gccgcgtcga gatgcggcat aatcgcctgc
     8161 tcgaagcgct gcagtttttc gttacctcgc aacgttaaga ccgctcctgc gctgacttaa
     8221 ttccccaaac caatcggaag ttaaaaattt tcggccgcct gattgccgca aactctagtg
     8281 ccgtggctgg gaagagtcaa tgaagactaa ggctgctggt ccatcttact aaatcgtccg
     8341 gcagttgcct gcgtgtttgg gaattacgat cgatcgtcgc tgccaatcaa tgaccgattt
     8401 ttcgagacag aaattcccca agagcaacca ataaagaata tagcacctaa aggaatttcc
     8461 tcacggaatt cggtctaaag ggcaaaggcc gctcacagcg cgacgaatga ggagaaccat
     8521 aaaacgtaga acgagcattt tatctgccgt cttatggctt gcaggcatgg ggccgaatgt
     8581 agcgtctgcc ggcgatgagg cgatgattgt gtctaaagac gctctcgtca ttggcagcta
     8641 ttgccacatg aagtttccag caattcgtga atagacgtta gtcacggctc acccgatgct
     8701 taaggaagcc ggccccggtg acatcatcga tttttacgga ccgtgtgacc atgccccgtt
     8761 aggcatcgac gaagtgcaat cccaaaagct taaacctcca gcatcgtcga acgcgcgaat
     8821 gaatgccgga gataagtcgc ggcggaattt atttcctttt gatggcgtcg tgcgctcgcg
     8881 gcaatggctc gatcacgagg cttggtggcg tggtggcata gcctttggtt cgcgtcccgg
     8941 tgcgcctggg catgcctcac ggtccgcctg cgtcgtggtc acctcgtggt cgccattggt
     9001 gtgaatcgag actgtatgtg ccaagacata tgccggcaaa atttgcagaa aaattctgtg
     9061 tcagggaatt ttttcagccg cgctccggtc aggcagagta gaacacgcaa gttaaagaga
     9121 aaggaggtga tggaaatgaa gagcatcaag ctactcatga acgtcgtgtt tctgacagga
     9181 tctatggttt tcagcgcagt gtcgagtcgg gctgaggtga tctcaaaaaa acctttaacg
     9241 atagcagtta ctctgacacc aagttcgcaa atgtcgaggc tggcattggg tcaaccagac
     9301 gctcgaaaag acctgccccc cggttgcgaa atgctgggaa tccaacgacc tcaagcaagc
     9361 gagcgataac tgtaaccgga agaatttcgt tatcggacgt aactctaatt ttatggagga
     9421 attatgagaa ggaagaaaca aatgaaactg atgctgagcg gttttggata tctggatgtg
     9481 ttgatcgcgg gcgttctact gttgggcgcc tctagccaga tcgtagagcc ggcatctgcc
     9541 gctccggcag caaaagccta cgttgcaaat ttcacagaca acacggtgag cgtcatcgat
     9601 acggaatccg caaaggtgat cgcaaccatt ccggttgctg ccgggccgca cggcatggcg
     9661 atcaccggag acggccgcac actgtacgta agcggcgacc gttcgtcctc actgaacgtc
     9721 atcgataccg ctgtcgacag ggttgtaaaa acaatcgata tcggtaagac gccaaacggc
     9781 gtggcgctta cgcccgacaa tcgcttgctg ctcgttacca tatacgacga agaccgtgtg
     9841 gtgttcgtcg atccagcgac acagagtgct attgccgccg taccggtttc aaagccgcat
     9901 accgtgtcga ttagccccga cggcaagttc gcgtacgtta cggtacagga accaggccac
     9961 tttgggttag ctcgaatcga cttgaattcg cgcagcctcg ttcacactct gcctctcgac
    10021 aagacgccgc gcgatggaga attcagcttc gatgggaaga tgttttactt tactcaggcg
    10081 ggagtcaact cggtccaggt gctcgatccc gcgtccgaga agattgtcgc gcaaatcccg
    10141 accggcgtat caccgcattt cgttggtcat ccagttggct cggccttcgg cattgtcgtg
    10201 gtgcaggggc cgggtgaact gtccttgttc gatcccgcga cgcacaagcc gactcgcaac
    10261 gttagcgtcg gcaaacagcc acactgggcg gctacgtccc aagatggcaa aaccacgtat
    10321 gtaaccgatg aaggttccaa tgatgtgacc gtcgtcgaga tggctaccgg cgcgaccaag
    10381 acgattgcgg ttggcaatgc gccgcgcaag gttgtcattc agccaatggc ggcaaggaag
    10441 gaaagctaag atgagaaatc acaaccacag catcgataac ttgcacacgt tgttcactgc
    10501 cgtgatgagc gcgttgtcga gcgggctttt tgcatttgtc gttttgacgc agccggcgca
    10561 gcaaatcttt gctgcgccag ctggcgatct tggacaggtc cactcatcgg ccaacacgtt
    10621 cgcacagcga gcagcaacgg cccccgttga gaacggcgca aaggtctcca tcgttaactt
    10681 cgcctttaca ccggaacaga taacgataaa cccaggtgaa acgatcacct ggatcaatga
    10741 tgacggcgcg ccgcatgcgc tcaagtttca ggacggtgct aagggtacgg atctgatgtt
    10801 gccgggtgca acctttaggc gtcacttcga tggacccgga acctacgatt acagttgttc
    10861 ggttcatcct tatatgacgg gtcgtgtggt cgtaactatc aggtgatgga gttcaaccct
    10921 taagacagtc cgaaaagtac ccaagcatag ttcttaagcc gcccgtcggg acaaggcttc
    10981 caaaaaagtt tctcactgat gctgatcccg aatgactacg agctttgttc taaattcgaa
    11041 agtttcgatc ggtccgcgac gttcaaagct acgataatcg gtcattcaag tgcacggata
    11101 caatacccag agggatttcc aacgctttgt catttccgaa ggtggatgaa tcttctctca
    11161 gcagatgtct catgcctcaa tcaaacgctt tcctgacagg cgcggctata cacggttcgg
    11221 acaaccgcag cggaatatcg ggcgagcgcc gaagagataa gggattcata tgttgaccgc
    11281 cggttggtgc cgacgatatg cgtacgaaga attcaattgt tgtccatgga tgcccgccag
    11341 ataacaattt cgtcaatgac ctccctcaaa ggcagttggc tgatactcaa ccaggggagt
    11401 tgtaacacga aataagatca cgaaaacaaa agctttgcat cgaacgcttg gagccggcca
    11461 ggtttcgtcg ccgatggcct tggtcttgcg gtggaagcga gacgtctcgt cgccctaagt
    11521 gcggggcctt cagctcgagt atcgtttgat cactcatggt taaggagaag atgtaatgac
    11581 cggcgcggaa attgtcattg gaataattat cggagtgcta ttcgaactct ggtcgaatac
    11641 cgaagggacg ccgcatgagg atctaactgc tcaaggcgtt gcaaagtgat gacggccgct
    11701 actttgtatc acgcgaagcg gtggcagaac tcccccactc tggttcatcg taaatcaaag
    11761 tcctcggccg ttacctttca aagctagcgc gcaactcgcc gcattgcttg ctttggtggt
    11821 taacgagaat aatgcttgcc ttgtctctgc ggcgctgaca tttcacggat ccggtttgga
    11881 tgaaccgcca tagatctagc gcgtggcata caaaccggag ctaaaacctc ctgggctgaa
    11941 cttctcacca ggcaccataa atctctgcca ggcccgttaa atctttgctg catcgtggca
    12001 tgataaaggc atgcccagag caaatggcgg ccggacactc tttgatgccc cggcgcaagt
    12061 tccggatgga tttatctata acgaagactt catctcagag accgaagagc aggagttaat
    12121 ccatgagatc cgaaaccttc cgctcgagcc gtttaagtat tatcgattca ccggcaagcg
    12181 gcgaacagtt agtttcggat ggcaatatga attcggcgct agtgaaatca cagaagctcc
    12241 agagatgccg aactttcttt taccgctgcg gacacgcgcc ggtaatgtct tcaacattga
    12301 cccgaacagt ttagttcaaa catcgatcat cgaatattct actgggtcgc cgattggttg
    12361 gcatcgggat attccgcagt tcggtattgt catcggggtt tcccttggag cggcatgccg
    12421 gatgaggttc cgcaaatttc gccgcgttcg atccaagaag ttagatcgtg atcaagtatt
    12481 atcgatcgaa ctgcatcctc gctcgatcta tttgatgagc ggagcttcac gagaggtttg
    12541 gcagcattca attccggcgc taaaggcgct tcgctacgcg attatgatgc ggactctacg
    12601 cgtcaagtct gaaagggctc gagcataaac cgaaatggtt gggtagtgcg aaacaccatc
    12661 actccttgcc ggtccaatat tcaacatcga tcagaggctg ttgattgcat attcaatcca
    12721 gatccaatta taacgtagat tcccgacgga gacgcgcaag ggagatcggg gatgggagcg
    12781 gaatgtaaag cttgcgcttc gccgaagctc ttgttcacta gacttgaaga caaaatgaga
    12841 aaacatcatg cagcatctag ctaaaatccc ttttcccgaa gttgaacgtc tttcccaggc
    12901 gggcaaagca ttggttatcc tgccggtggg cgtggttgaa gagcacggcg cgcacttgcc
    12961 gttgggagtg gactcttttg ccgccgaagc gtacgccatt gcggcggcgc cgcatctcga
    13021 agaaaaaggc tatgaggtga tcctggcgcc gacgatcgat tatggcgtgg cgcgccaagc
    13081 cttgaatttt gccggaaccc tgactttgca accgaaaacc cttgagtctc tagtcgtcga
    13141 tatcgggcgc tcgctggcaa agcatagatt ccgtcgcctg gtgattctca acggtcatcg
    13201 cgatctgggc cacatgaaaa ccttggaggc cgcccgcagt gttcttgtgg ctgaggaaac
    13261 ggcgcaagtt ctttgtacgg gtttcaccag cgatccggtg gtaaccgcgg cgtgctaccg
    13321 ggaaggcgtc aagcagtttt atcgcagtcc gaggcctgat cgcgaggggc acggcggtga
    13381 atccgaaacc tcggtcgggc tgtattgttt ccccgaactg gttagacaag aactgatcgg
    13441 ccagctcgag cccaacttcg attatgacgt cgaagcattt cataacgaga ccgaagatta
    13501 tgggattctt agcgacggcc gggggtactt cggctcgccg caagcagcaa gcgcggaaac
    13561 gggaaaacag ttggtattga ctcgtggccg caatatcgcg caggtgattt taaacgcctg
    13621 gggtgctctt tgaaaccgtc gaagacgcca tccgatcgcg actaactggc gtgcggcatc
    13681 gccgcgttat cttcgaaacc accaggcgcc aagcggttcg agatggcggg tagtgagatc
    13741 ctgctaaaag aaccacccgc tgtcctgcac ggctgatttg tggatgccgc tgcgcgccag
    13801 attcaagacg gctatggaag acgaggagcg tttgcagcca gcggagcgcc agcatcattt
    13861 gaaacgataa tgagctcttg tccaggacgg agatgccagc tcttgagacg gttctctttt
    13921 accagctgct taggtgttac attgtatttc tttgcgatgc gcgccagcaa ctcgcccttt
    13981 tttactctat gaagactcaa tttgatttta tccggacctg ttaactgttc atagcccgct
    14041 agaaaatctg cctggcttcc taccggcagc cggagtgtga aggctccgtc cgtcggcggt
    14101 gtgaagttct gcagcaaagc cggattgagt tctttgatga cgtctacgtc ggtgttcgca
    14161 agacgagcga ctgtctcgag tctcaagggg tgattgatgg tcacctcgtc atattccatg
    14221 ggtgattcat aggagatttc ttcgaaacca tatcgctccg gttggcgcgc gatgagagct
    14281 gcggcaatga acttcggcac gtaatcgcga gtctcgagtt tgaggtgttt tcgctcctgc
    14341 cgtaggagcc agtaatcatt tgtgttggaa cgttgtaggg cagttccaac cctgccttcg
    14401 cctgcgttgt acgctgctgc ggcgaggaac cattcgccga acctctcgtg caaatccttg
    14461 aggtaggctg cggccgcgcg tgtagagagc accggatcgc ggcgctcgtc taaccatgag
    14521 ttgatcgtca acccatactg aagcgctgtg ccacgcatga actgccatgg ccccaaggct
    14581 tgagcgcgtg aataggcgtg agtagaaaag ccgctttcaa tcagggcgag atagacaagc
    14641 ttgtccggga ggccctcact ccggagaatt tccgccatca tcgagcgata cttgccggag
    14701 cgcgctaaag cacgctcgaa aaaatctctc atacggccgc aaaacccttc aacgtagaaa
    14761 cgaactcgat cgtgtgcctc gacggctggg gagatcagaa gaatgcgttg ttcggcgggt
    14821 tcctgcatcg ctcggtccat gtcacggcgc cacaattcga tcaagtcgtc gtcggacgcg
    14881 gcattcggca gttcatcaat ggtgcgggtg tccgccggag ttttgtgctg ctgttcgaga
    14941 ctcgcggttt tttctacggt tttctgtgac aacgaggccg aactgacttc agcattcgac
    15001 ggcaaatctt tcttttgcgc tcgaatggcc ggggccgatg cggtttgact ctcggtgacc
    15061 ggagcagtgt taatcgccat ggacgaatgg agacctgctt catcgcggcc ggttggatct
    15121 atagggagag gatgtgacag cgagcaaccc agcagggccg cagcgagcca agagtagaca
    15181 aagatgccag gcagtgaacg catgattcga tccagaagca tccgatcggc aatcgctggc
    15241 tttgccatcg gtattcaagc ggactggccg cgagcccgct taggggcaag cttcatgcca
    15301 gacgggtcaa agggttcttt tcaaatagct ttcgaccttc gcaaataacg attccagccc
    15361 cggtcgcctg acaaaaaaag ccggagggga caattttggc gggaggtaag ggaggggata
    15421 atcatccgtt cacatgtaaa ggaaaaaaag atcaacgacg aattggctcg acaatcctcg
    15481 cctcggcgca gttctcgaat tctaatgctg gattaagtcg acgaacagtt cgattgctgt
    15541 aacttgtgtt gcgccgtcat cttgttcaac gaagcgatac tcaagatgac cgagcgtaat
    15601 ggcggggctt aactctagct gcttatcgca ccgaggccgg tgcgagaccg atgtttgtca
    15661 gccaatttat cgtctcttct gtcaccattc gctcattatt cacgaacagg tgagcttgaa
    15721 atgaggaatt gccggggtga gaaggaatga cgacgatatc ggctctcggg caattgaccg
    15781 cactttcttt gatccgcgcc gcaatggttt cccgtcgagt gatgctgccc tttccctgga
    15841 tgttgtcggc acgatcataa acaatcagca gcggcaccct gatattcgcg atcatttttt
    15901 gcacggcggc tttgctggtc tcgacatcgc ggtagctgag aaatgtcttg gcggaggtga
    15961 tgatcacttt gccttcacgg aatggcagta atttaatctc ttcgcccttg ccgctcgaaa
    16021 ccaaggtgcg gcattccgcg gcaaagttct cgtatagctc ggaaccgagc agggactctc
    16081 ttgtgacgct tggaatatcg atatgcgggc cgtaaacgcc caacgcttta actgaaggat
    16141 gcggtttgtt cccttgataa tattcgatta tcggggtgcc caggctatgt ccggcgagaa
    16201 agatgttttt gaagcctcgc ccattcgcaa aagtgatggc atcctcaacc tccttcactg
    16261 cggcttcaaa ctctgaagtt cgaaagctct ctcctgaatg gacgccattg taggcgagcg
    16321 ctgcataacc ggcttcggcg agataacatc cgagaaacat cgttggtccg gaataccagt
    16381 acgcttcgac tccgtgcacc agtaccaccg cagtgccggc ctcgtgaaat agctcggggt
    16441 ccgggccgaa taagcctccc tgcacagtcc tgccggcgct ttgaaattca atcagctcaa
    16501 agctgacacc ttttttcggt aaataaccca tctggcaaat taccttcctt ctctaaaacc
    16561 aagagcgtaa actctgatca atcagatgag tcttttcccc atcgccgctt gttaccacga
    16621 tcagagtgac ggta
//
LOCUS       DASUVS010000322         8279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1445556, whole genome shotgun sequence.
ACCESSION   DASUVS010000322 DASUVS010000000
VERSION     DASUVS010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8279
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445556"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1232)
                     /locus_tag="VF182_17230"
     CDS             complement(132..1232)
                     /locus_tag="VF182_17230"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX6438882.1"
                     /translation="MMLYKSIIRPWLFSKDPEQSHDRMLDLLSRLDFLHDPLDRFYRI
                     ADNRLNVQIKSLMIPNPVGLAGGFDKNAVALKTISAFGFGFMELGAVTAQAQPGNPKP
                     RLYRLPEDCALINRLGFNNEGATAIAARLAKLRALGKLPGIPLGINIGRTKIVETKDA
                     VADFLSTFEKLFSHGDFFTLNVSSPNTPNLRELQEKTLLADLLSAVQEKNRQLASAAQ
                     REEKPVFVKIAPDMEFAQVDEIIDVVNSFGLAGIVATNATAFMRDSVKSPLGREPGGL
                     SGRPITTMVTNFVSHIYKNTRGRLPIIGVGGIFNAQDAYDKIIAGASAVQIYTGFVYE
                     GPAAVKRINQGLIKLLRRDGFKSVGEAVGQAN"
     gene            complement(1460..2077)
                     /locus_tag="VF182_17235"
     CDS             complement(1460..2077)
                     /locus_tag="VF182_17235"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX6438883.1"
                     /translation="MILEMRTYVLKPGMTANFVEKFAEGLTARLQFSRLLGLWQSEVG
                     GLNRVVHVWPYESFEDRERIGNEARKTGMWPPKTQEFIVTQENKILQPAPFSPPLPET
                     KQGNIYEFRTYTYQPGTMPKVLEAFGKVIPERAKLSPLIGIWVSAIGPLNQFIHVWAY
                     RDAGERERVRAEASKKVSGWPPETRQYMVMQENMLMNPSACAPFK"
     gene            2435..3895
                     /locus_tag="VF182_17240"
     CDS             2435..3895
                     /locus_tag="VF182_17240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6438884.1"
                     /translation="MNLFARKQSEDSQTAADQSGLQQTTPSAPVSWKDFGAAQQREAD
                     ENRSSTISQRQHRSFYLPVKGKFVLSTAFACTWVFLSWNLAQPWLSELAKDIGYVPAV
                     VIIFFIALFPGFLNAHIISSVILDRPPELRFDIEYPPINLLIAAYNEAQEIAETIRGI
                     KGQDYPGPIDIIVVDDGSTDKTISILNSFDLPNLKIVQANHGGKAKALNEGLNHVSHD
                     IVVCIDADTFLQPEALKRIVARFLSDPQGTAAIAGCVLVKNSRDTFMTRLQEWDYFTG
                     IASAKRQQSLYQGTLVAQGAFSAFNSKIVKAHKGWPSVIGEDIVLTWALIKSGWRIGF
                     EPTAIGFTAAPATLNGFFRQRKRWARGMIEGLKQHGHLIWSSSRLSAFFVGIDFVIPL
                     MDLFYTFVFLPGIILALFGYYYIVGPTTLLVLPMAFLIVFFMYRKQQIVFKTLRLRVR
                     QNFAGFIVYMLIYQIIMSPICVIGYVQELLGTAKRW"
     gene            4143..5666
                     /locus_tag="VF182_17245"
     CDS             4143..5666
                     /locus_tag="VF182_17245"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6438885.1"
                     /translation="MILIFMSCSPILAAESAESVDALQSRIKATSDRSMLARLHKQLG
                     DQYIAQDRISEAAEAYGHALEMDREQFSSAERVQMAVYLSWADNLKQSERELRAVLAG
                     QPKHLEARTHLARVLSWQGRLDEAIEEADNVLKEHPNNREASLVKADALQWRGDYTEA
                     IPIYRQLSTDGNFDAGVGLSRSLLATGNRTASVETMNSLKAGNSRQERELRRLRETVD
                     QETRPSLDARYNYHSDSDDNRLNRYSLSANFSYGNQKLGMTFRHTDAEDKTRDNRAED
                     LSFRLYTNPTDWLTLGGGLGFTQLRDGRTSTFPTGHLRIDTRLFRGTAGANVHREVLS
                     DTAELIENRIRMSDVGLYLSQPLTDRLSVYAGYNYKDFSDGNHANDIQLASQYAIYLN
                     PRINVGYRFRFLDFHEQSGSGFFDPSNYIANRVFTSVWLERDKYYTYLEGYFGYETFR
                     RNRVASDNFIYGGSGSIGYKPISRLAIEFNAEGGTFAAGSASGFNYFVIGPRVLFRF"
     gene            6249..6716
                     /locus_tag="VF182_17250"
     CDS             6249..6716
                     /locus_tag="VF182_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6438886.1"
                     /translation="MEKKIKLRVNGKKHELEVPTNRLLIDCLRYDLGLTGTKEGCSVG
                     VCGACTVLMDDEMISACITLAVMADGHEITTVEGLAQNGELHPVQKSFIQYGGFQCGI
                     CTPGQVVAAKALLDENPKPSEDEIKDWMMGNLCRCTGYYKIIESIQKAHQFAR"
     gene            6727..7659
                     /locus_tag="VF182_17255"
     CDS             6727..7659
                     /locus_tag="VF182_17255"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6438887.1"
                     /translation="MIPFEYRTPKSLREVHATLKEFGSDAKLIAGGTSLVIMMRQRLV
                     RPNCLVSLRSVRGLNSIEIHDGGTRIGGLATHREVESSSLLRRRLPLVAETYHHVATI
                     RVRNMATVGGGLAHADPNQDPPPTLIALGGTVKATSADGSRVIPLDEFFTDYYETVLK
                     PDEIVTEVFIPKMAPNSAGAYLKFLPRTADDYATVSAAAVLTLDKTGKTINDVRIALG
                     SVGVTPIRATAAEAVLRGQPLKAEAFVEAGEKAKEAVDPVSDFRGSAAYKKEMAGVFV
                     RRALEKALATLRQQTRLKSAPKAIKKAVKKGKKR"
     gene            7656..8105
                     /locus_tag="VF182_17260"
     CDS             7656..8105
                     /locus_tag="VF182_17260"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6438888.1"
                     /translation="MKFSQSAQIPVAREPLWEFLMDVPKVAKSLPGVESVSKIDDTTY
                     QGALTVRVGPIKLNLQGKIIVEEQNKENWRAALRAEAKDRMAAGAVNGKTNMVLKELG
                     PNQTELLVETDVNILGKIGEFGQPNIRKKADSMLKEFVENIKKQLTA"
BASE COUNT         2018 a         2153 c         2152 g         1956 t
ORIGIN      
        1 cggccgcttt ctaattgaga agcacaaaat cagaacagat tttgtcgaat ggcaacaaga
       61 aatgccgttt atggatatcg atgtgcgaga ggattacgaa cgattaaagg aactggcata
      121 actcaaaaaa ttcagtttgc ctgcccgacc gcttctccga ccgatttgaa tccatctcgt
      181 cggagtagct tgatcaagcc ctggttaatt cgcttcaccg ctgccggacc ttcatagaca
      241 aaccctgtat agatctgtac cgcagacgct ccggcgatga ttttgtcata ggcgtcttgg
      301 gcattgaaaa ttccgccgac gccgatgatc ggcagccgtc ctctggtatt tttgtagata
      361 tgagaaacaa agttcgtcac catcgttgtg atgggcctgc cgcttaaacc gccgggttcc
      421 cggcccaacg gtgatttgac gctgtcacgc ataaatgccg tggcgttagt agcgacgatg
      481 cccgctagcc cgaacgaatt caccacgtcg ataatctcat cgacctgcgc aaattccata
      541 tccggcgcga tcttaacgaa tacgggtttt tcttcccgct gtgccgctga cgcaagttgc
      601 cggttttttt cctgaacagc gctgagcaaa tccgcaagca gcgttttttc ctgcagttcg
      661 cgcaggttcg gtgtgttggg tgaacttacg ttcaaagtaa aaaagtctcc gtgtgaaaac
      721 agcttttcaa aggtcgaaag aaaatcggcc acggcgtcct tggtctcaac gatcttggtc
      781 cggccgatgt tgatacccag cggaattccc ggcagcttgc caagagctct caacttggcc
      841 agtctcgcgg cgattgcggt tgccccctcg ttgttgaacc cgaggcggtt aatcagcgcg
      901 caatcctcgg gcaagcggta caatcgcggc tttggatttc ccggttgagc ctgcgccgtc
      961 acggcgccca attccataaa cccgaatccg aaagccgaaa tcgtcttcag tgccactgcg
     1021 ttcttgtcaa acccgcccgc caggccgacc ggattgggaa tcatcaagga tttaatctgt
     1081 acgttgagtc gattgtcagc gattcgatag aagcgatcga gaggatcgtg caggaagtcc
     1141 aatcgtgaaa gtaaatcaag catccggtcg tggctttgct ccggatcttt actaaacaac
     1201 caaggacgaa taatagattt gtatagcatc atcaaaggat ctagccgaaa gtatgtcgct
     1261 gggctagttg gctaaacgat tcgcagactg cgaagtcgta caacgaaaga gggagaacgg
     1321 ttggttctcc ctcttgcagt aattaaccgg ccagttggaa cgccgcccga tatcctagtg
     1381 ccgtagtgaa gagttttcag atctgcagcc ggaagaatca gctcactcaa cgtgcgtcag
     1441 aggcggcgca tagccgtatc tatttgaaag gtgcgcacgc cgatggattc atcagcatgt
     1501 tttcctgcat aaccatgtat tgcctggttt ccggcggcca gccggaaact ttctttgaag
     1561 cttcagcgcg aactcgttcg cgttcgccgg cgtcgcgata tgcccacacg tggatgaatt
     1621 ggttaagcgg cccgatggcg ctgacccata tgccgataag cggcgaaagc ttggcccgct
     1681 ccggaatcac tttgccaaag gcctctagga ctttgggcat cgtgccgggc tgataagtat
     1741 aagtgcgaaa ctcgtagatg ttgccctgct tggtttcagg cagaggcggc gaaaatggcg
     1801 caggctgtaa aatcttattt tcctgcgtaa cgatgaattc ctgggttttc ggcggccaca
     1861 tgccggtctt tctggcctca ttgccaatcc gttcacggtc ctcgaaactt tcgtagggcc
     1921 agacatgaac gacgcgattg aggccgccca cttcggattg ccagagaccc agcagacgtg
     1981 aaaactgcag gcgtgcggtt aatccttcag caaatttttc aacgaagtta gcagtcatgc
     2041 caggtttcaa cacataggtt cgcatttcca agatcataag ttttcctccg ttctgagggt
     2101 ttcctatcct ttcccacaaa gcggtgaaca aatgcaacag ctccgcgcac tcaaatccgt
     2161 gaaccgcttc tcatttagat aagcagtggc agagcgagac tttttgcagc caagcatgat
     2221 cctccgaact cttcgactcg tcccagggct agcgatgagc acggattgcg caacttgtca
     2281 tttttgtcga gagcccaaga ctttctgaca ttgttcgcgg ttgaaaactc gcgcaactgg
     2341 cgagaacgcc ccgggtgcgc ctggtatgtt tgttgctcga tcatgcgcgt tcgtaacccg
     2401 aaaggagagt tgttctcgaa cttataacca caccatgaac ttattcgcac gcaaacaatc
     2461 cgaagactca caaacagcgg cagatcaatc tgggctgcag caaaccacgc cgtcagcccc
     2521 tgtcagctgg aaagattttg gcgcggctca acaaagggaa gccgatgaga atcgatcgag
     2581 cacaatttcg caacggcaac atcgatcgtt ctatctgccg gtcaaaggca agttcgttct
     2641 ttcaaccgcg tttgcgtgca catgggtttt tctatcctgg aatctcgccc agccttggtt
     2701 gagtgagctc gccaaggata tcggctacgt gccggctgtg gtgatcattt ttttcatcgc
     2761 gttgtttccc ggcttcttga acgcgcatat tatttcgagc gtcattctgg accgtccgcc
     2821 agagctccgc ttcgatatcg aatatccgcc gatcaatctg ttgattgccg cttacaatga
     2881 agctcaagaa atcgccgaaa ccattcgcgg tatcaaggga caggactacc ccggcccaat
     2941 agatatcatc gtcgttgatg acggttccac ggacaaaacg atcagtattc tcaactcctt
     3001 cgatttgccg aatctaaaaa tcgttcaggc gaaccacggc ggtaaagcca aagctctgaa
     3061 cgaaggactg aatcatgtga gtcacgacat cgtcgtttgt atcgatgcgg atacctttct
     3121 ccagccggaa gcgctgaagc gaattgtcgc gcgttttttg agcgatcccc agggtacggc
     3181 tgcgattgcg ggatgcgttc tcgtaaagaa ttctcgcgac acgttcatga cacgtctaca
     3241 ggagtgggac tactttaccg gaatagcatc ggctaagcgt caacagtctc tctaccaggg
     3301 aacattggtt gcgcaaggag cctttagtgc cttcaacagc aagattgtca aggctcacaa
     3361 gggttggccc tcggttatcg gagaggacat cgtgctgaca tgggcgctca tcaaaagcgg
     3421 ctggcgtatt ggcttcgagc ctaccgccat tggctttacc gccgcaccgg ctactctgaa
     3481 tggttttttc cgtcagcgaa aacgctgggc gcgaggcatg atcgaaggat tgaagcagca
     3541 cgggcatttg atctggagca gttctcggtt gtcggctttt ttcgtcggaa tcgatttcgt
     3601 tattccgctg atggacctgt tctatacgtt tgtatttctc cccggcatca ttctagcgct
     3661 cttcggctac tactatatcg tcggtccgac gacactgctg gttctaccga tggctttctt
     3721 gatcgtgttc ttcatgtatc gaaaacagca aatagttttt aaaacactcc gtcttcgggt
     3781 gcggcaaaac tttgccggct ttatcgtcta catgcttatc tatcagatca tcatgtctcc
     3841 gatctgtgtt atcggttatg tccaagaatt actaggaact gccaaacgtt ggtaagaact
     3901 gcaccgctgc atggcgcctc ctgaacaacg ccactctgaa atcgatcgca atctctccgg
     3961 cgcatacacc taagcctcac tttcacgggc gcaaaccgta gaaaaatgta gaccactgaa
     4021 aatgtttatg gcattttagt gaatctactt acttttagta ccttgaggaa actttgatcg
     4081 aatcaaaact aattgatccc gttctgcgac agtatagggt cgtactcgca gcgactttga
     4141 caatgatact aatcttcatg tcatgttcgc cgatcctggc cgccgagtct gccgagtctg
     4201 ttgatgcgct gcagagcaga atcaaagcca cctcggaccg atcgatgtta gccagattgc
     4261 acaagcagct gggcgatcaa tacatagcgc aagaccggat cagtgaagcg gctgaggcct
     4321 acggacatgc gctggaaatg gaccgggagc aattctccag cgcagaacga gtccaaatgg
     4381 ctgtctattt atcgtgggcg gataacctaa aacagtcgga gcgcgagctt agagcggtgc
     4441 tcgcaggaca accaaaacac cttgaagccc gcactcacct cgcgcgcgtg ctatcctggc
     4501 aaggtaggtt agatgaggca attgaagaag ctgacaacgt gctgaaggag catccgaata
     4561 atcgtgaagc gtcgcttgtc aaggccgacg ctttgcaatg gcgaggagac tacacagagg
     4621 ccattccgat ttatcggcaa ctgtctacgg acggtaactt tgatgctggc gtggggctat
     4681 cgcggtctct tcttgcaacc ggcaaccgca ccgcttccgt agaaaccatg aactcattga
     4741 aggcaggcaa ttctcgacaa gaacgcgagc ttcgaagatt gcgggagacc gtcgatcagg
     4801 agacccgtcc ctccttggat gcccgttata attaccacag tgattcagac gacaaccgcc
     4861 tgaaccgtta ctcgctttcc gcgaattttt cctatggtaa tcaaaagctg ggcatgacct
     4921 tcaggcacac agatgcggaa gacaagacac gtgacaatcg agctgaagac ctctcgttcc
     4981 gactttacac caatccgaca gactggctta cgcttggcgg cggcttggga ttcactcaac
     5041 tgcgggatgg ccgcacctca accttcccga cgggtcattt aaggattgac acaaggttat
     5101 ttagaggcac ggctggggct aacgtccatc gggaggttct tagcgatacg gctgaactga
     5161 tcgaaaatcg tatacggatg agcgatgttg ggctttacct atcgcaaccc ttaacagatc
     5221 gactttcggt gtacgctggt tacaactata aggatttttc agatggcaac cacgccaatg
     5281 atattcagct ggcatcccaa tacgcgattt atcttaatcc gcgcattaac gtcggatacc
     5341 gctttcggtt cctcgacttc cacgagcaaa gcggcagcgg atttttcgat cccagcaact
     5401 acattgccaa ccgcgttttc acttccgttt ggttagaacg agacaagtat tacacctacc
     5461 tcgaaggtta tttcggctac gaaacgtttc ggagaaaccg cgttgcctcc gacaacttca
     5521 tctatggcgg ctccggcagc atcggctata agccgatttc taggttggca atagagttca
     5581 acgccgaggg tggcaccttc gccgccggct cagcatccgg cttcaactat ttcgttatcg
     5641 gaccgagagt tctgttcaga ttctaagtcg ccgaacgtcg ccagctagac cagcacgaaa
     5701 cctttagcca aaccaagcgg tgcttcatcc tgaatgagca ttcctctcgg ttaaaaagct
     5761 cggctcggca atagccgctt cccgaattag cgcctgctcc atcctgacga gtcgaaattc
     5821 atttaacaca agtgagtaga cgcgtggtta ttttggttag cgtctccgcc ggaattgccg
     5881 ggacagagta gtttacaaat caatcaattt taataagaag ggcggcaggc ggaattgcta
     5941 aaagagcttg tttcgtgcca gcctcatccg ctggatcaga tcaatgaagt gttcgagttg
     6001 atgcacaaag gaaaaagcag tcgctcggtg attcactttt gatttggcgc ggccaaacat
     6061 catgccttgc aagaagatcc ttaaaatcaa gccgatataa atccactgcg tgattacgga
     6121 cggggatgaa ttcgggccgc tctgccgcgg gaacggcttg cagtttagac aatttctttc
     6181 tataagttag gcccagtact aatccgctca ctacaaacca ttttgcaaat tgtgtgaaag
     6241 ggaccgcgat ggagaagaaa atcaaactgc gcgtgaatgg caaaaaacat gagcttgagg
     6301 ttcccaccaa tcgattattg atcgactgtt tgcgttacga cttgggtttg accggaacca
     6361 aggaggggtg cagcgttggt gtctgcggcg cctgcacggt gctgatggat gatgaaatga
     6421 tcagcgcttg tatcacgctt gcggtcatgg ccgacgggca cgagatcacg acggttgaag
     6481 gtctggctca aaacggtgag cttcacccgg tgcagaagag cttcattcag tatggcggtt
     6541 ttcagtgcgg catctgtact ccaggccagg tggttgctgc caaggctctg ctcgacgaaa
     6601 atccgaaacc cagcgaggac gagatcaagg attggatgat gggcaacctt tgccgttgca
     6661 ccggctacta caagattatt gaatcgatcc agaaggcgca ccagttcgca agataaggac
     6721 acagccatga taccctttga atatcgtacg cctaaaagcc tcagggaagt gcatgcaacg
     6781 ttgaaagaat tcggctccga tgcgaagctt atcgccggcg gcacctcgct tgtgatcatg
     6841 atgaggcagc ggttggtcag gcccaactgt ctcgtgagcc tacggtccgt gcgcggcctc
     6901 aacagtatcg agattcatga cggcggcacc cggatcggcg gattagccac acaccgcgaa
     6961 gtggaatctt cttctttgct gcgtcgcaga ttgccgttgg tcgcggaaac ctatcaccac
     7021 gttgcgacga ttcgggttcg taacatggcc acggtgggcg gcggattggc ccacgctgat
     7081 cccaaccagg atcctcctcc cactttgatc gcactgggcg gaaccgtcaa ggcgacgtcg
     7141 gccgacggta gtcgggttat ccccctggat gagtttttta ctgactacta cgaaaccgtt
     7201 ttaaaaccgg atgaaatagt caccgaagtg tttatcccca aaatggcgcc gaacagcgcc
     7261 ggcgcttatt taaagtttct gccgcgtacc gcggatgatt atgccaccgt ttccgcggcc
     7321 gccgtgctta ctctggataa aactggaaag acgatcaatg acgtgcgtat tgcgttaggc
     7381 agtgtcggag tgactccgat tagagcgacg gcggcggaag ccgttttgcg gggccagccg
     7441 ctgaaagcgg aggcttttgt cgaggccgga gaaaaggcga aagaggcggt tgatccagtg
     7501 agtgatttcc gtggctctgc agcctataaa aaggaaatgg cgggggtttt cgttcgccgc
     7561 gcgctggaga aggctctggc gaccctgcgt cagcaaacta gattgaagtc ggctccgaag
     7621 gcgatcaaga aagccgtcaa gaaagggaaa aaacgttgaa gttcagccag agcgcacaga
     7681 tcccagttgc ccgtgaaccg ctgtgggagt ttttgatgga cgttccgaaa gtagccaaat
     7741 cgcttcccgg cgtggagtcc gtcagcaaaa tcgatgacac cacctaccag ggagctctta
     7801 cagttcgagt ggggccgatc aaactgaacc tccaaggcaa gatcatcgtt gaagagcaaa
     7861 acaaagaaaa ctggcgcgcc gcgctgcggg ctgaagccaa agatcgaatg gccgcaggcg
     7921 ccgtcaacgg caagaccaac atggtgctaa aggaactcgg ccccaatcaa actgagttgt
     7981 tggtcgagac cgacgttaac atactcggca aaatcgggga attcggccaa ccgaacattc
     8041 gaaagaaagc cgactctatg ctcaaagaat ttgttgaaaa catcaagaag caactaactg
     8101 cctgatagcc gtctgttttc cgtccggtga cgaattcacc ggacggtgag tttcctgcct
     8161 gtagaccaac tcaacctaga cgctggcagc aagagcgcga gttcatcgat acgcgctggt
     8221 cggcaagccg gcggaccgca tagaactaac gatgctgcca atcgggcttc cttttttct
//
LOCUS       DASUVS010000323         3985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_353784, whole genome shotgun sequence.
ACCESSION   DASUVS010000323 DASUVS010000000
VERSION     DASUVS010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353784"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VF182_17265"
     CDS             <1..716
                     /locus_tag="VF182_17265"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6438889.1"
                     /translation="IGVWVGIVPGIGSQVVDWLAYGHAAQTSKDAKNFGKGDVRGVIA
                     PESANDAKDGGDLVTTLLLGFPQGVVTALFIVALLAWGYLPGPEMVKKNLDIIYSVVW
                     IQGISGVIGTLIGFVLAAQLAKLAEVRYSVMVPIIFIFVLMGAFSVNRDPIDLLVVVV
                     FGIIGYLMRRFGYPRPAMILGLVLGGLMETYLYRSMASYGFSWLARPSVIVLLSLALI
                     SFLLTMRSRLKSAPEAPAA"
     gene            738..1211
                     /locus_tag="VF182_17270"
     CDS             738..1211
                     /locus_tag="VF182_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438890.1"
                     /translation="MNYLFTYFLFFIFLAATVAGWSWPYIAKLMPVYVAAIPGLILII
                     AQLARDAFGKAPAAETTGGSIDMDEVYDVKLEKSVEVRRTLIFFGWFIAGAVGIWLLG
                     IVIALPVLVLLYTLIEGKESWITSLMMTVGTYLLIWGLFEYMLETRWPAGWLFGY"
     gene            1349..2083
                     /locus_tag="VF182_17275"
     CDS             1349..2083
                     /locus_tag="VF182_17275"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6438891.1"
                     /translation="MQIIEKEFTTKEHGVAGFLSHPERKTPGPALLLIHPKGGMTDYI
                     KIESRKYARLGYSTFAINVFEQLGYPAATHIETGSQIQAKTADPEFTRVLTEGWRFLL
                     SQPHVNRQRVAVCGYCMGGRIAIHFVAATPEVRAFVGYYPTVRDESTSALRPVHPSEA
                     VGKIRCPSIVLYGADDVTTVLSVQEKMWQAFKSNSQPLEWHFFPFGGHGYVDPGAVGY
                     HAHAAELSWPLVVDFMERHLQWGDAA"
     gene            2154..2573
                     /locus_tag="VF182_17280"
     CDS             2154..2573
                     /locus_tag="VF182_17280"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6438892.1"
                     /translation="MDKITKPTVVDTEKQAQWGRATGRNYLRPTQTDRAHIALNCYEP
                     GANDEMHCHPGSDHTFLVIEGECTMKGLNEGEEYKLTQHQAVHVPAGYFYQLNNTGSG
                     RLILYQVSTEPNPRPKIGKVLYGIDTSLKEHLLEPVG"
     gene            2587..2841
                     /locus_tag="VF182_17285"
     CDS             2587..2841
                     /locus_tag="VF182_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438893.1"
                     /translation="MTFEQVLNWCRDNGADARGIYRARDFWIRRSDDRLPDNLPPMGA
                     LFHWDVQISGTQLAVSPSDMERMVGGKMSLEQFKATHRRG"
     gene            3092..>3985
                     /locus_tag="VF182_17290"
     CDS             3092..>3985
                     /locus_tag="VF182_17290"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HEX6438894.1"
                     /translation="MELILEIHPITAIRFGPSTKLDGSSLVVDTDVLQALLLEDQTLT
                     AVDLAIASPGESCRAGPVFDIIEPRAKTADSSPDFPGILGPPHTAGLGTTHVLEGAAV
                     TVLREKSPGDSRGAPGYVLEMSGEAAVGSKYGSLQHLIIVPKTQSDLPDHARHKAYRL
                     AGLKAAVYLARAAFNDAPASRLKFDTLDAIARERDGLPRVAYVGQIFSRQRRPELDEA
                     ILYGLGTDGMLPVPLHPDEWLDGALLPSYHTSLGGAETYFYQNHPVITELYRRHHARE
                     LNFAGTVATIASADNFDRERNC"
BASE COUNT          830 a         1074 c         1147 g          934 t
ORIGIN      
        1 tcatcggcgt ctgggtcggc atcgttcccg gcatcggctc gcaggtggtc gattggcttg
       61 cctacggtca tgccgcgcaa accagcaagg acgcaaagaa tttcggcaag ggcgatgtac
      121 ggggcgtgat tgcgcccgag agcgccaatg acgccaagga tggcggcgat ctggtgacga
      181 cgttgctgct gggatttcct caaggagtgg tcaccgcgct ctttatagtc gcgcttctgg
      241 cctggggata tttaccgggc ccggaaatgg ttaaaaagaa cctcgacatt atctatagcg
      301 tggtttggat ccagggcatt tccggcgtga tcggaacttt gatcggcttt gttttggcgg
      361 cgcaattggc caaactcgcc gaggtccgtt actcggtcat ggtgccaatc attttcatat
      421 tcgttctgat gggcgctttc agtgtcaatc gcgatcccat cgacttgctg gtggtcgtgg
      481 tgttcggcat catcggctat ttgatgcgac gattcggtta tccaaggccc gcaatgattt
      541 tgggcctggt cttgggcggt ctcatggaga cttacttgta tcgctcgatg gcgagctatg
      601 gttttagctg gctcgccagg ccgtcggtga tcgtccttct cagcctggca ttgatctctt
      661 ttcttctgac catgcgttca aggctgaaga gtgcgcccga agctccagcc gcttaggcgc
      721 ggggcggaga gccgatcatg aactatcttt tcacctattt cctatttttt atttttctag
      781 ccgctaccgt tgcgggctgg agctggccct acattgccaa gttgatgccg gtttacgttg
      841 cggcgattcc cggtttgatt ctgatcattg cacagcttgc gcgcgacgct ttcggcaagg
      901 cacccgccgc ggaaaccacc ggcggttcaa tcgatatgga tgaagtctat gacgtcaagc
      961 tcgaaaaaag cgtcgaagtg cgacgcacct tgattttctt cggttggttc atcgccggag
     1021 cggtgggtat ttggctgctg gggattgtca tcgccctgcc cgtgctggtt ctgctctaca
     1081 ctctcatcga agggaaagag agctggatta cttcgttgat gatgacggtg ggcacctatt
     1141 tactgatttg gggcctgttc gaatacatgc tggaaacacg ctggcccgcc ggatggctat
     1201 ttggctattg atcatgccgt tctcgaatct tgccgtgtaa acgttcttgg ccgcccactt
     1261 ccttattaac cgtgcccaaa ccctttacgc atgtctgcgg atcatgttag gtatcgggga
     1321 atagcttacg gcgacaagga ctaacagaat gcagatcatc gaaaaggaat ttaccaccaa
     1381 agaacacggc gtcgccggtt ttctgtctca tccggaacgc aagacacccg gccccgcatt
     1441 gcttttgatt caccccaagg gtggaatgac cgactacatc aaaatcgaat cacgcaagta
     1501 cgccaggctg ggttacagca cgtttgcgat taacgtcttc gagcaactcg gctacccggc
     1561 ggcgacccac atcgaaaccg gatcgcaaat tcaggccaag accgcagatc ccgaatttac
     1621 ccgggtgctt accgaaggct ggcgcttttt gctatcccag ccgcacgtga atcggcagcg
     1681 agtagcggta tgcggttatt gcatgggcgg gcgcattgcg attcatttcg ttgccgcaac
     1741 gcccgaagtg cgcgccttcg tcggctacta tccgacggtg cgcgacgaat cgacctcggc
     1801 cttacggccg gtacatccgt cggaggccgt gggcaaaatt cgctgcccgt cgatcgtttt
     1861 gtacggcgcc gacgatgtca cgacggtatt gtcggtgcag gaaaaaatgt ggcaggcgtt
     1921 taaaagtaac agccaacctt tggagtggca tttcttccca ttcggcggcc acggttatgt
     1981 cgatccgggc gcggtcggtt atcacgccca cgccgcagag ttgtcgtggc cgttggtggt
     2041 ggatttcatg gagcgccatc ttcagtgggg cgacgcagcg tagcttcatt gcaattttgc
     2101 agaaaaggat aaaccgttga ataagtctgt tcgtaaactc atggaggttc ccaatggata
     2161 aaataaccaa gcccaccgtg gttgataccg agaaacaggc gcagtggggc agggccacgg
     2221 gcaggaatta tctccggccg acgcagacgg atcgggccca catcgcgctc aactgttacg
     2281 agccgggagc caacgacgag atgcattgcc atccgggatc ggatcatacg ttcctggtca
     2341 tcgaaggcga gtgcaccatg aaaggactaa acgagggcga agaatataaa ttgacccagc
     2401 atcaggcggt gcatgtgccg gcgggttatt tttatcagct caacaacacc ggttccggac
     2461 ggctgattct ttatcaggtg agcaccgaac cgaatccgag gcccaagatc ggcaaggtgt
     2521 tgtacggcat cgataccagc ttgaaagaac atctcttgga accggtcggc taacctggag
     2581 gaagagatga cattcgaaca ggtgttgaac tggtgtcgtg acaatggagc cgatgccaga
     2641 ggaatctatc gggcaaggga tttctggatt cgccgctccg acgaccgtct gcccgataat
     2701 ctgccgccca tgggcgcgtt gtttcattgg gatgttcaga taagcggaac acagttggcc
     2761 gtcagcccct ccgatatgga gcgcatggtg ggcggtaaaa tgagcctcga gcaattcaag
     2821 gccactcacc gccgcggttg accctcttag tgttagcgat aagaccgctc aagtccgtca
     2881 caaagccgca gcaagatttg ctgcggcttt ttacttagag ctgtcgatgg cgtgaagcca
     2941 gattggcttg gttggagcgt ccaatcgaat aagtaaaagc caaccgccgc cgatcgtcgt
     3001 gctttgcatc atactttcgt cggcttggtg tcgttgacaa gtccggagcc cacacaatac
     3061 agtccaggag atcagtagaa agttacttgc gatggagcta attctcgaaa ttcatccgat
     3121 cacggcaatt cgattcggtc catcgaccaa gttggacgga tcctcgctgg ttgtcgatac
     3181 cgacgtgttg caagcattgc tccttgaaga ccaaaccctg actgcagtcg atttggcgat
     3241 tgcttctccg ggagaaagtt gccgggccgg tccggttttc gacattatcg agccgcgcgc
     3301 caagacggcc gattcgagcc ccgattttcc tggaattctg ggaccgccgc acacggcggg
     3361 attgggcaca acccatgtgc tcgagggcgc tgccgtgacg gttctcagag agaaatcgcc
     3421 cggagattcg cgcggcgccc ccgggtatgt cttggagatg agcggcgaag cggcggtagg
     3481 atcgaagtac ggctcgttac agcatttgat tatcgtgccc aagactcaat cggacctgcc
     3541 cgatcacgcg cggcacaagg cttatcggct cgccggactc aaagccgccg tgtatctcgc
     3601 gcgcgcggca ttcaatgatg cgcctgcctc acggcttaaa tttgacaccc ttgatgccat
     3661 cgctcgagag cgtgatggcc tgccgcgtgt cgcctatgtc gggcagattt tttcgcgtca
     3721 gcgcagaccg gaattggacg aggcaattct ttacggtctt ggcaccgacg gcatgctgcc
     3781 ggtgccgctg catccagatg aatggctcga tggcgcgttg ctgccatctt atcacacctc
     3841 cctaggcggc gcggagactt atttttacca gaatcatccg gtgataaccg agctttaccg
     3901 gcgccatcat gcgcgcgagc tcaacttcgc cggcaccgtc gcgacgattg catcggccga
     3961 caacttcgat cgcgagcgta actgc
//
LOCUS       DASUVS010000324        29976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1008569, whole genome shotgun sequence.
ACCESSION   DASUVS010000324 DASUVS010000000
VERSION     DASUVS010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 29976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29976
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008569"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..294)
                     /locus_tag="VF182_17295"
     CDS             complement(4..294)
                     /locus_tag="VF182_17295"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEX6438895.1"
                     /translation="MSIVRLIYVSVKGDQVKDAVNLWKEHCAPLMIKQPGCLSEKLLE
                     CVDAPNEFISYSEWDSQESIDKYRNSEAHAEIQRHSRGLQGGQAIVKRYAVK"
     gene            complement(633..1796)
                     /locus_tag="VF182_17300"
     CDS             complement(633..1796)
                     /locus_tag="VF182_17300"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438896.1"
                     /translation="MASEIAYRFISADDHIDLRWLPKDLWTERLPSKLRQLGPRVVEN
                     EKGSYWTWEGQTFSPHGYYTAAQGSGAMWAIERAGVMREGELRPTTAALRLSDMDRDG
                     ADASIMYGPTDPMVIADPELRQRCYEAYNDWLIEFCAAKPARLIGVPQLSMEDPIAAR
                     AELERLAKRGGLRHVNILASRANPPVYDQRWEPFWAVAEESGIPIGFHLAVLVKKTRL
                     DEADRRAANLVVTAASRFAKEPPGIQLLEPITGLIFSGVLDRHPRLKIVMAEAGLAWV
                     PSMIQGLDIWYQRTRDGRRLTGDSPVSLPKMLPSEYFHRQIWISFVDEPLGIKMVGSV
                     LDVDKVMFGSDYPHPASTWPHSQHIIEGQTQAMSPDIAKKIFRDNARLLFAVE"
     gene            complement(1786..2781)
                     /locus_tag="VF182_17305"
     CDS             complement(1786..2781)
                     /locus_tag="VF182_17305"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438897.1"
                     /translation="MRQWSIIIFFIGILLSRPTEIFSAETALKIINVAVPAVSLLQAP
                     LFTALDAGAFKKYGMEVRYVVTGARTIQALIGGSVQFAQGVSSRTVPAAVLAGADTVL
                     IANFSDKLLFTMHSAPEVKSLQDLRGKVVGVSGIGGSTDFATRLALREAGLIPEKDVA
                     IRGVGGVPETVAALKAKVVQAGTLSPPSSFVAQKAGFKMLFDMSSLGVDYISSGLGVK
                     KAAIASNRQEVKQFLMAMIEGAKILTSDEEFALRVLGKHTRISDREILKQSYSYLRPY
                     FLKVPYPSARAIKDTLDILSKEIPRAKDADPGEFIDNSVVKEIEASGYIENIYGK"
     gene            complement(3614..5191)
                     /locus_tag="VF182_17310"
     CDS             complement(3614..5191)
                     /locus_tag="VF182_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438898.1"
                     /translation="MKHHLLCITQRRVVHTACAAVSLAILAFSSSALAASATQIPGMG
                     PVPKADCGRFDWTESGLQGETTTWERESGDSEGGYNCNLELVGQFRGEGAFSQNGPAY
                     YDHCAYMSTENDPLQQNPGVVVIDVSDPRHPRPTAYLDDTPAGLNPHENVKVNQKRGL
                     LGLAQSNGPNFAVYDLSKDCAHPILASSITVPDSRGHMGGWAEDGKTYYIGQQFRGAG
                     GIMPIIDVSDPYNAKWLLNWTFDGDGRPHDLSTNKDGTRLYSGQPGNFGVAANNSSFG
                     PDGLVILDVSDIQFRRPNPEIRVISTLFWDDQGQAEQMLPISVNGRSYIISTDESGGQ
                     GGVGGLAAACARGASPYGYSNIIDITDEKNPKIVSKIMLEVHDPANCEHFLTEPPEVG
                     GGALDYSTERCAPDRPDNPTMLACGSRGTGTRVYDVRDILRPKEIAYWKGPAPRTAFL
                     PGSGSWSPGVDRTVEKQAGLARFVKVPATNGKGRELHLWVVGDAGGFQVLRFTDSFTQ
                     TKAGKAIHADALQHSAE"
     gene            complement(5342..5740)
                     /locus_tag="VF182_17315"
     CDS             complement(5342..5740)
                     /locus_tag="VF182_17315"
                     /inference="COORDINATES: protein motif:HMM:NF014543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438899.1"
                     /translation="MKRLSSAFFCGAFLMTLGAPAMSHDCRVRDGYLRGAYEGDCDER
                     TELAQGMGKAKGADKYAGDFAKGRPDGKGIYTWQSGAHLEGNFNEGKAHGPGIYVSAK
                     GARYEGEFVSGKLEGLKNDDCPSTPGPLTC"
     gene            complement(5860..7506)
                     /locus_tag="VF182_17320"
     CDS             complement(5860..7506)
                     /locus_tag="VF182_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438900.1"
                     /translation="MKQDACITHSRVLRTALSAATLAILAFSSGAMAAGVSQSGEQKN
                     MQRVGHTDLQGRPGYQPNVIEYPDGRWIAFSGTHSNIPVARPGFTFLPNPLNNNADEP
                     NGTMIIDVTNPNDPREMSHIPVPVAGGQAQMARLCLGSDLNEDLRGKVYLMRNIQGNT
                     AQMSGYEVWDVTDVKKPVLASALRGIRSTHKHWWECDTGIAYMPGSKDSGPLWRQAQS
                     MVIVDWKNPTAAPNYIRTFGLVGGQPGSTGPIATSLHGPISAHEHPFAANRLARGATA
                     DDIIGNRVYAAWGVGDDGVLQILDRKKLLPAAYGGTWVPAKPDSADAPTEQELIDPIA
                     GILYMSPDQGGHTSFPVFGLKPPSYQAFTEFQTRDIVLLASESTADTCDEAPHWSFVV
                     DVTVENSKFAPPGTRVQQNVFQGPMVLSTMSVDPFEGEKYPRGNYCGRGARFGVHSSE
                     ENFWNPLYGKLTSIAYFTGGVRIWDIREPHAPLEVAFYVPESNANTNPDGYMTNNVEI
                     DDRGFIYIVDRNGAGFDILQLTGKAADIVYPARPRPRPRK"
     gene            complement(8887..9873)
                     /locus_tag="VF182_17325"
     CDS             complement(8887..9873)
                     /locus_tag="VF182_17325"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX6438901.1"
                     /translation="MARTPIFNREQAITRLHETVHGKADNFYAMYSSILGGIVNDPAI
                     MVLPLDDHMVHRGHGVFDTATLTHGMLYQLDPHLDRLIRSAHGARIPLPFSREELRQI
                     ILDTAAASRQRDGSVRYWLSAGPGGYGLGPAECVGSSFYVIVFKQEAYPESYYHDGIK
                     LVTSRVPIKPPLFARIKSTNYLPNVLVLLEAKEQGADNGIFIDERGMVAESSNMNVAF
                     VTRDRVFRHPPFEAILSGITIQRVLEFAMRLVQQDVLSAIDLAAIPVQEGLQAAEMML
                     VGSSIKIAPVIEWDGQKIGDGKPGPVAKKLLEMWNEDATSAKDHLVAVPYDD"
     gene            complement(10108..10596)
                     /locus_tag="VF182_17330"
     CDS             complement(10108..10596)
                     /locus_tag="VF182_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438902.1"
                     /translation="MKAQAQRDRQLQINIGMHGPALAILTLLFALRVLGQALVVFFSV
                     DWLPPSEHWFSGLIPYPILLGVQLVMLAVMVKIASEIRRGRGFFAKPRPGCCRFLVAC
                     SILYAASMILRYILTMTYRPDMRWFGGTIPIFFHWVLAAFLYTWGNFNSGRDNLIAQR
                     HR"
     gene            complement(10556..12241)
                     /locus_tag="VF182_17335"
     CDS             complement(10556..12241)
                     /locus_tag="VF182_17335"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEX6438903.1"
                     /translation="MRLLVKAAAFVTRRQWTRWRKLTAKPQETQDELLLEILARNRAT
                     RFGHDHRFKSMRSLSDYRHQVAIADYERLRPYVELINNGERDALTAEPVDMFTMTSGS
                     TGEPKLIPVTAKTRQNHRQLTRLWYYRALVDHADLFNGKLLGVISPAVEGKTTSGIPF
                     GAASGLIYRSSPRWIKNAYAVPYDACEIKDFEAKYYLIMRLALEQDISFFGTPNPSTI
                     LKLVQSASQNKYDIIKDIHDGTISSRFDLPAQLRASLASRLGKNQLRARRLESLIKHD
                     GALRPKEYWPRLQLIGCWKGGSAGVRLKEFAGWFAKTTPVRDLGYMASEAQMTLPVFD
                     SGSAGILTIDKNFYEFIPESEINSGAPTILTCAELEEGNLYYIILTTPGGLYRYDIND
                     VVRVVGFYNQTPLIDFVRKGRDVTSITGEKLHVNHIIDAMARAQRAAGVAVQHFRACA
                     DVEKYLYVFSVELDGVAPSTETLVQLLQELDSGLRELNIEYAQKRESRRLGAPVLCVM
                     KPGWFERKAQIILQRGGRDTQFKAQLLCVTPEDPDEIQFVVESAGAARSTTPD"
     gene            complement(12248..14119)
                     /locus_tag="VF182_17340"
     CDS             complement(12248..14119)
                     /locus_tag="VF182_17340"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX6438904.1"
                     /translation="MISPEKITQTFESATRQFRQSDEYQELVAGRANLEAAREFLRNV
                     FRTHFLSSHIVALCFASLPSSSAELLRENLLEEMGRSDLEKPHSALLLELARGVGFDQ
                     SEIDRLIDDARRRLAVFCAARIPALTLRELCLAVLIETMSFEFMLSRCASEIAAALTS
                     HYGFAKTALHWFALHSEVDVRHAEEGVTVIQDYLRFHQASDDLFEQIASLTLGENLFA
                     RHYFPAGSKQRTGARSRPVRARRIEAMTIYRLRIPFHQTFRHSLHSREESDAVIVKII
                     DSDGRRGFGESLPRSYVTGETTESMLARIRERLAPRVFSQSFAPGWESLEYLQSVMPE
                     WTKPEQGQSSVAAWNAAFCAVELALLDWSLRADHCALADLLPPARYEVVYSGVISADA
                     PDDAAALATRMARIGICQMKVKLGTADDAARLEAVRKAVGGNVELRADANGAWAPDEA
                     IQQLQRLARFNLQAIEQPVRADDLMGMRRVRQESGVPVMADESLVTLDQGRRLIELGA
                     CDYFNIRLSKNGGIAGSLAIATVAREAGIKIQVGAQVGETGILSAAGRIFAAHLPALA
                     FAEGSFGTWLLAEDVTFENIAFGLGGRAPLLQNRGLSVTVKEEVLERLAASKIDLHR"
     gene            complement(14377..14772)
                     /locus_tag="VF182_17345"
     CDS             complement(14377..14772)
                     /locus_tag="VF182_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438905.1"
                     /translation="MKAFVAAFLIVFVAGISAPSDVPANEFKEREAKIAQYKTWLDSL
                     GSNGSQYWTRLDARPRPHRLYLGEGFYRADVQSQERFVDTFSNFLAGHPEKFMLIDLF
                     DAATNKPVGEYGFGGFKLYTKPLQTAERP"
     gene            complement(15016..15804)
                     /locus_tag="VF182_17350"
     CDS             complement(15016..15804)
                     /locus_tag="VF182_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6438906.1"
                     /translation="MSTATQPSAFNRHGVWAIYKFEMARALRTLLQSVVTPVMTTSLY
                     FVVFGAAIGSRMTEVNGVAYGSFIVPGLMMLSLLTQSISNASFGIYFPKFTGTVYELL
                     SAPVSSMEIVLAYVGAAATKSIVLGLIILSTAALFVPITILHPLWMIGFLVLTAATFS
                     LFGFIIGLWAKGFEQLQFIPMLVITPLTFLGGAFYSIDMLPPAWRTVTLFNPVVYLIS
                     GFRWSFYGTSDVSVGVSLAMTLGFFAICLAVVAWIFKTGYRLKS"
     gene            complement(15801..16727)
                     /locus_tag="VF182_17355"
     CDS             complement(15801..16727)
                     /locus_tag="VF182_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6438907.1"
                     /translation="MPPVISISNLTKTYASGYQALKGIDLEIRKGEIFALLGPNGAGK
                     TTLISIVCGIVTPTAGIVLVDGHDIIGEYRAARSKIGLVPQELTTDAFESVWATVSFS
                     RGLFGHAPDPAHIEKVLRDLSLWDKRKNKIMTLSGGMKRRVLIAKALAHEPEILFLDE
                     PTAGVDVELRRDMWALVRTLRETGVTIILTTHYIDEAEEMADRIGVINKGALIVVEEK
                     VELMKKLGTKQLTLSLLEPLPSLPAELSQWPLALKDGGRELEYTFHGDDESTGIPMLL
                     KGLGDLGIGFKDLNTRQSSLEDIFVSLVSDRK"
     gene            complement(17135..18571)
                     /locus_tag="VF182_17360"
     CDS             complement(17135..18571)
                     /locus_tag="VF182_17360"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438908.1"
                     /translation="MAKITADILFKNGLIVTPSGVIEGGVGIVDGKIAVVGGDAFLPD
                     AEQIIDLAGKHLLPGVIDPECHLGGHRDLAADFKSETRAAAATGVTTWGLQLVSLAIT
                     GGADKVKGPGDIPNFTKSMPIFYEAAKSAAIDFFLTPIVSTDEHVNEIPYLAHEHGIS
                     SFKLHLQMQGPWKSSWPAYFFDDGSIYSVFKAVAKLGAPAIALLHCENWEIAQVLQKQ
                     LIAAGRNDMGAWDDRSPAFTEVGHARTYLYYARIAGCPIYVVHCTTTETVAEIERAKV
                     EGLTVYSEIGTHYLVLHKDAWKINVPLRDRSTHESLWKALSSGAIDCIGSDHVAHTRT
                     RESMETGNVWTTISGFPSRVEGMLPMMLSEGVNKGRISIERLAQVASENPARIFGLYP
                     KKGAILPGADADLVVVDLKRRAKITKELLHTVTPWSVYEGWDVTGWPVMTVVRGNIVM
                     EWPEGAPRARVTEASTGQYLRRQLSRTT"
     gene            complement(18575..19732)
                     /locus_tag="VF182_17365"
     CDS             complement(18575..19732)
                     /locus_tag="VF182_17365"
                     /inference="COORDINATES: protein motif:HMM:NF025694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate cyclase domain-containing protein"
                     /protein_id="HEX6438909.1"
                     /translation="MKNEELSYNLGDTVDRLISVQYFMSGGGSALTRKPVTGVLYEAA
                     REKAGAPLCHLAAKALADNVERGDTVLITTGLIAPPYMIAEGDGPIAAATLARAIDVG
                     LGATPVIVCEGVVVKAMEALCQAAGLMPSAPELAVGTPRKVIVLPFPLDDDLGRREAI
                     RLLDQFKPKAVVAIEKLSKNAKGIYHNGFGIDVSSVQAKVDYLFDFAGDRGIPTIGVG
                     DGGNEIGTASIAEAVRRVVPTGAQCGCPCESGIAAATAADYCVFGSKSNYGGFAIEAC
                     LAAGLERPEILHSEVVGRRVHEAGATMGLVDPFTGLAFGWDDFPVEKNVNELVVNLMR
                     YMLAVRVVPRWYKDSIMGPSQKWIHERSFAETLVSRWAKSIIDSEKQFFSK"
     gene            complement(19746..20741)
                     /locus_tag="VF182_17370"
     CDS             complement(19746..20741)
                     /locus_tag="VF182_17370"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438910.1"
                     /translation="MTKTSSALKYIFWFAALVLINSATRTDAQQSPKLTPINYGTITI
                     SALHWPYLIAEQEGMLQKEGIEIKRVMGGTTTATSQALVGGSTDLAQMNLVQLLAANS
                     AGADLIAVAGDTWVPIYALIVHPSINSYADLKGKRLAVASPTDPLNYVLARMLAANGL
                     TPADYEMIGLGGTPTRLAAVEKGGVAGALVNQPSDFKAIASGLRLLGYSSDYVDNFQY
                     TVTGARRDWAQANRPLMVRFLRAFVRACDFFYEPKNKEAVLRALIEKAKAERDEAEKT
                     YSLFIKTKKSIPRDGGIDITGARIVAESWKEFGLQKPPPPVDGVIDMSYLQEARK"
     gene            21053..21562
                     /locus_tag="VF182_17375"
     CDS             21053..21562
                     /locus_tag="VF182_17375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6438911.1"
                     /translation="MIHSELSLRLARPAEAESIATLSRDLIEYGLDWRWTPVRVAASI
                     RAANVNVLVASMHEDIAGFAIMRYGDDDAHLDLLAVSPSHRRTGIGRQLLEWLEECAV
                     VAGIFNVDLEVRAGNEEAQLFYTRMGYRPLLQLPGYYQGVEAALRMGRDLSRRSLDQR
                     LTGSNPKSC"
     gene            21778..23238
                     /locus_tag="VF182_17380"
     CDS             21778..23238
                     /locus_tag="VF182_17380"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6438912.1"
                     /translation="MTRRGEEYVRGLHDGRTVLLNGERVPNVTTHPAFAAGIRSIANL
                     YDLAADPANRELMTYPSPRDGRPTNKSWLVPRSRDDLAARRRAIKFWADASYGLLGRS
                     PDHVASFFAGFAGAPDFYARGGQQLADNLSRFAAKAADEDLYVSYVIVHPTVDRSKPA
                     HQQAEPYLYAGVAAERDNGIVIRGAQMLGTGSVMSDYVLLTVILPLKPGDEDYAISCV
                     VPNSASGLKLYPRRPYALGANSIFDYPLSSRFDETDSLVVFDDVLVPWEDVFIYKNIE
                     LTAGQFSVTAAHVLGNTQSHIRSWAKLQFLVGLIKRCMDLSGRSANTEIVAQLGDLAT
                     RVSLIEGMILGAEAAAEPDAFGVMRPKDSLLYASQVFQQAMYPALLNQIRGLMGGSLI
                     QLPATVAELRATPSSGDIDRYIRWPKAGARDRVKLLKLLWDAVGSEFGSRHLQYEMFY
                     AGEPAAIQGREFRTFDWAAAEALVDRCLATYDESTA"
     gene            23495..24001
                     /locus_tag="VF182_17385"
     CDS             23495..24001
                     /locus_tag="VF182_17385"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX6438913.1"
                     /translation="MKRKKHILTAVFISGTIGAGVNGLFAQSIPGPTVPKPVPEVPQQ
                     TQPTLPGQPAPGLPPTEPLPGQRGTIPERMQAPDAGSQRDMVISSEDIKKAQEALKAK
                     GKDPGAVSGRMHAKTQEALREFQKENNLPATGVLDQKTAEKLGVTLKGDEGSKPQQRQ
                     QGAKPEAK"
     gene            complement(24157..25005)
                     /locus_tag="VF182_17390"
     CDS             complement(24157..25005)
                     /locus_tag="VF182_17390"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438914.1"
                     /translation="MMIIDAQVHIWAEETPEKPYAKGNASQPHRLIPLGHEELLGEMD
                     GAGVNRCVLVPPTWEADRNDTSLEAARSHPDRFGVMGRLKINAPESRALLATWKDQPH
                     MLGIRMVFNRGRSREWLDDGTADWFWPAAQRYDIPVMAFAPGAVPKLAAIAESHSGLR
                     MIIDHMGLSTALRGKPLDSAVDDLIKMARLPNVAVKVSALPCYVEEAYPFPTLHPLIH
                     RVVDAFGPQRCFWGTDLSHLPCSYRQAVTLFTEELGFLSATDKEWIMGRGIAAWLNWP
                     FKSGTR"
     gene            complement(25228..26661)
                     /gene="hemG"
                     /locus_tag="VF182_17395"
     CDS             complement(25228..26661)
                     /gene="hemG"
                     /locus_tag="VF182_17395"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX6438915.1"
                     /translation="MSRRIAIVGGGIAGLAAAHRLIELRNAQASDLEILLLEASHRLG
                     GCIATERVGDFLIEAGPDSFISEKPWALRLCDRLGLAPRLVSTQAAHQKIYVVHNGKL
                     VPLPEGFFLLAPTQLAPFIRTPLFSWRGKLRMAAELLMPRGDANMDESLGAFIRRRFG
                     NEALERVAQPLLGGIYAADPDKLSLISTMPRFKQMEKSQRSVIWAMWSEQRRRARKRE
                     GGSGARWSLFVTLAGGMQEMIDVIAQRLPQASPRLNTAVRSLFPSDGKNKWQIATGTG
                     EILEADGIILAAPAFHTARILASAVPEAAKELSGITYASSATISLAYRNRDFPRIPDS
                     FGFVVPAIESRKMMACTFSSLKYPGRAPEGHLLLRAFVGGSLQPDFLHDNDAILERNV
                     RAEIGNLLGVNAEPLLVRIQRHPNAMPQYHVGHQARIQRIESALGKYANLALAGSAYH
                     GVGIADCVHTGEEAAEKLFKQIDFSKS"
     gene            complement(26722..27654)
                     /gene="hemH"
                     /locus_tag="VF182_17400"
     CDS             complement(26722..27654)
                     /gene="hemH"
                     /locus_tag="VF182_17400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX6438916.1"
                     /translation="MVSYQAVLLIAFGGPTSPDEIRPFLARVTQGTAIPPQRLEDVAR
                     HYEAVGGKSPLNEITARQARALQRLLSEHGMDLRVYIGMRNAAPFLADTLRQMAGDGV
                     ERALGFILSSHHTDASWERYQRNVADARLELGRAAPEIDYCARWHDDPLFIQTWCELI
                     RESFAGIAREQRPATPLVFTAHSVPVSMADRSPYVEQLAITARAIAEKIDHKNWSIAY
                     QSRSGNPADAWLEPDINSVIRDLSVKGASNVVIAPIGFVCDHVEVLYDLDIEAQKTAA
                     QAGIELHRASCPNDHTTFIKMIAAVIAKSVQASQ"
     gene            complement(27910..28953)
                     /gene="hemE"
                     /locus_tag="VF182_17405"
     CDS             complement(27910..28953)
                     /gene="hemE"
                     /locus_tag="VF182_17405"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX6438917.1"
                     /translation="MRNRSIQNHPFLTACRREAVPHTPVWLMRQAGRYMEDYRTLRAQ
                     YGFLELCKKPDLAAEITVTPVERLNVDAAILFADILLILEPMGVGLEYSKGDGPVIHR
                     PVRSGKDVDGLKDFDPEKELSFVYEAVKKTCAALDDRVPLIGFAGAPFTLASYLIEGA
                     GSRNYIHAKKLYYTAPEVWHRLMQRLSQLITKYLNCQIAAGVQAVQLFDSWAGCLTPG
                     DYEQFVLPYTRSVIAGITKGVPVINFSTGTAGLLKHIRAAGGNVIGLDWRINLDEGWA
                     TVGHDVAVQGNLDPVALFAPPKEIRSRVADVLDRAGNRPGHIFNLGHGVLPETPVDHV
                     IAMVDAVHELSAR"
     gene            complement(28996..>29976)
                     /locus_tag="VF182_17410"
     CDS             complement(28996..>29976)
                     /locus_tag="VF182_17410"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX6438918.1"
                     /translation="EARKFGVRSAMPGFQANKLCPHGIFLPNRHRLYSEYSHKVFAIL
                     RQYSPEVQAISIDEGVVDLTGTEKLLGPPLQTADRIIRRIEQELGLPSSGGVSSHRVV
                     SKIAATLAKPQGLIFVAGGDETNFLATLPVELVPGVGPKTQKELLARGIRTIGDLMNS
                     KLGSRHLDLGQVEHRQRSHEHSIGNETTFEPLQGFEQLERIIWQLVEEVGCRLRKENY
                     YARCLTVKIRYSDFQTITRSRTLNSPTCYDREIFETVKQLLQQNLAQDRAVRLLGVSA
                     SALQSSGWQESLLDRDKRSSWDKLYHGIDHLRDKYGDDAIGAAGPRKPRS"
BASE COUNT         6413 a         8503 c         8465 g         6595 t
ORIGIN      
        1 ggattacttc acggcatatc gcttcacgat agcctgaccg ccttgcagtc cccgtgagtg
       61 tctttgaatc tccgcgtgtg cttcgctgtt tcgatacttg tcgatgcttt cctggctatc
      121 ccattcagag taggagataa attcatttgg cgcatcgaca cattccaaga gcttttcgga
      181 cagacacccc ggctgtttaa tcatcagcgg agcgcaatgc tctttccaaa gattaactgc
      241 atccttaacc tgatcaccct ttacgcttac gtaaatgagt ctgacaatcg acatggcaac
      301 ctcctttttc atcttaccca aacaatcttt gcctgctcat cacgaccaac atttccccga
      361 ctcagcacaa tgatatttaa ttcaatgcac gcgcacggag cttaatcaag cgggtttttc
      421 atttagatac gtaatttagt cgcgagatcc atagctggtg cgctcacccg ttgacccctt
      481 gggcgggttg ctgagattta gctagccgca aatgattgca gcacgccagt gcgccagaac
      541 tcggtaagat cgaactatgc gatcagttgt tcgtgaatga tctacgggcg gatgacccct
      601 agcggtcgcg tagaaggtca aaatggaaac ttttactcga ctgcaaagag caagcgagca
      661 ttgtcgcgga agatcttctt ggcgatgtcg ggagacatag cctgcgtttg cccttcaata
      721 atgtgttgcg agtgaggcca cgtacttgcg ggatggggat agtcggaacc aaacataacc
      781 ttgtcgacat cgaggacact ccccaccatt tttatgccga gcggctcatc gacgaagctg
      841 atccaaatct ggcggtggaa gtattcgctg ggcagcatct tagggaggct tactggacta
      901 tcgccggtga gtctgcggcc gtcgcgcgtg cgctggtacc agatgtcgag gccctggatc
      961 atgctgggca cccacgcaag gccggcctcg gccatcacaa tcttgagccg cggatgacgg
     1021 tctaggaccc ccgagaagat cagcccggtg atcggttcga gaagttgaat gcccggcggt
     1081 tctttcgcga agcgggacgc tgccgtgacc acgagattcg ccgcgcgtcg gtctgcctca
     1141 tcgagccgcg tttttttcac caggaccgcg agatgaaacc cgatcggtat gccggattcc
     1201 tcagcgaccg cccagaacgg ctcccatcgc tggtcgtaga ccggcgggtt ggcgcgcgat
     1261 gccagaatgt tgacatgacg tagcccgccc cgctttgcga gacgttccag ctcagcgcgc
     1321 gcggcaatgg ggtcttccat cgaaagctgg gggacgccga tcaggcgtgc cggcttggct
     1381 gcgcagaact cgatcaacca atcgttgtag gcctcgtagc atcgttgcct gagctccggg
     1441 tcggcgataa ccataggatc ggttggacca tacatgatcg atgcgtccgc cccgtcacgg
     1501 tccatgtcgc tcaagcgaag agcggctgtt gtcggtcgca gctcgccctc gcgcatcacg
     1561 ccggcacgtt caatcgccca catggcgccg ctaccctgcg ccgccgtgta gtatccatgc
     1621 gggctaaagg tctgtccttc ccaagtccag tatgagccct tttcattttc cacaacacgc
     1681 gggccaagtt gccgcagctt gctgggcagc cgctcggtcc acaaatcctt cggcaaccaa
     1741 cgcaagtcta tatgatcgtc agcggagatg aaccgatacg ctatttcact tgccataaat
     1801 attctcgata tagccgctcg cctcgatctc tttgacgacc gagttgtcga tgaattctcc
     1861 gggatccgcg tctttggctc tcggaatctc ttttgacaga atatctagag tatctttgat
     1921 cgccctggcg gagggatacg gtacttttag gaaataaggt ctgagatagc tatagctctg
     1981 tttcaggatt tcgcggtctg aaatcctggt gtgcttgccc aatacgcgta aggcaaactc
     2041 ctcgtcgctc gttagaattt ttgctccctc aatcattgcc atcaggaact gtttgacctc
     2101 ctgtcggttg gaggcgatcg cggccttttt cacgccgagg cctgacgaaa tatagtcgac
     2161 gcctagactc gacatatcga acagcatctt gaatcccgct ttctgcgcga cgaacgaaga
     2221 aggcggtgaa agcgtaccgg cttgaacgac cttggctttg agcgccgcga cggtttcggg
     2281 cactccacct acgccgcgga tcgcaacatc cttctcaggg atgagccccg cttcacggag
     2341 cgccaaccgg gtggcgaagt cggtggagcc gccgatcccg gagaccccga caacctttcc
     2401 tctcaaatct tgcagcgact tgacttcggg agcgctgtgc atggtgaaca aaagcttatc
     2461 gctgaagttc gcaatcaaca ctgtgtccgc tccggccaga acagcggcgg gaaccgtccg
     2521 gctggaaacg ccctgagcga actgcaccga gccgccaatc aacgcttgaa tggtccgcgc
     2581 gccagtgacg acatagcgaa cttccatgcc atattttttg aatgcgccgg catccagcgc
     2641 ggtaaacagt ggcgcctgca acagggaaac cgccggaacc gccacgttga taatcttcag
     2701 tgctgtttcc gctgaaaaaa tttcggtcgg ccgagacagc aagataccga tgaaaaagat
     2761 tatgatgctc cactgtctca tcgtcgctcc ggtttcgcat tcaccttaag ccccgattac
     2821 tccaagcgag agagaatgtc tagcgaggtc cggatagacg tatacatcgc accggcgctt
     2881 gttctcacgg cttgagttca gccgtgatct tctgatcttc ctcaaggacc tgctgcggcg
     2941 aaaatggaag gttaggttat tccacttttc gaaagctaac ggggtttccg taagacgagc
     3001 caaataagac agagacttcc gctcactgtt gagagaattg gatttctgct caattgataa
     3061 gctgctttcg gaaagacaga tagaagagaa aagaccattt cactacaaag cagacgaaga
     3121 acgcgaagct tggaattttg ccagagagct gcttcggcgc cttcgtgaac tcggtatgag
     3181 ttttatgctc gagtgccgac tgagaggaca tcaacacaat cagcaggccg gccgaatagt
     3241 tttttaaaaa gattgcatga gttttcttat agctactcgt gttccgtgta cagatacgct
     3301 taacgtcagc ttcggggcta atggccagct atggctcatg tcatgtgcga gtaattaact
     3361 tcggcttgat cgagcggggt caaccgaaag ccaggcgata acgttgtatt catgcgcaac
     3421 gcccccgttc ggcacacctg gctcagcgct ttcgctccga gccaggtgtg aagcgaaccg
     3481 tccaagagag atggatgcgc gccgagcgaa accacccact ctcccgccat aaaggcgaga
     3541 gagtgggcat caagatcaaa gtttcaattt tgcgcctgcc tgacgctaaa cggccctgat
     3601 tgctgacgct cacttactcc gccgagtgct gcagagcgtc ggcgtggatc gccttacccg
     3661 ccttcgtctg cgtgaaactg tcggtaaagc ggaggacctg aaaacctccg gcatcaccga
     3721 cgacccaaag gtgcaactcg cgccccttcc cgttggtggc aggaaccttg acaaagcgtg
     3781 ccaaaccagc ttgcttttcg acggttcgat cgacacccgg tgaccagcta ccggatccgg
     3841 gaaggaaagc cgtccgcgga gccggcccct tccagtacgc aatctcttta ggacgtaaga
     3901 tatcgcgaac gtcgtaaact cgggtgcctg tacctctcga cccgcaagcg agcatcgtcg
     3961 gattgtccgg acggtccgga gcgcaccttt cagtggaata gtcgagggca ccgccgccga
     4021 cttctggtgg ctcagtaagg aaatgttcgc aattggcggg gtcgtgcact tcgagcatga
     4081 tcttggagac gatcttgggg ttcttctcgt cggtgatgtc gatgatgttc gaataaccgt
     4141 acggcgacgc tccccgcgca catgctgccg ccagaccgcc aacacctccc tgaccgccgg
     4201 actcgtccgt gctgataatg tacgagcgtc cgttgacgga gatagggagc atctgctccg
     4261 cttgcccctg gtcatcccag aagagcgtgc tgataacccg gatctccgga ttcgggcgac
     4321 ggaactgaat gtcgctcacg tccaggatga ccagcccgtc cggaccgaac gaagaattgt
     4381 tcgccgccac gccgaagttg ccgggttgtc ccgagtataa ccgcgtcccg tccttgttgg
     4441 tgctcaggtc gtgcggcctg ccatcaccgt cgaaagtcca gttgaggagc cacttcgcat
     4501 tgtacgggtc ggagacgtcg ataataggca taatgccgcc ggcgcctcga aattgctggc
     4561 cgatgtagta cgttttgccg tcctcggccc agccacccat gtgcccgcgg ctatcgggca
     4621 cagtgatgct ggacgccaga atcgggtggg cgcagtcttt ggacaggtca taaacggcga
     4681 aattcggccc gttgctttgc gcgagaccga gaagcccgcg cttctggttc accttgacgt
     4741 tctcatgcgg gttcagccct gcgggcgtat cgtccaggta cgcggtcggc cgtggatgcc
     4801 gggggtccga gacatcgatc accacgacac cggggttctg ctgcagaggg tcattttccg
     4861 ttgacatgta ggcgcagtga tcatagtagg ccggtccgtt ctgggaaaac gctccctcgc
     4921 ctcgaaactg gccgacgagc tccagattgc agttgtagcc gccctcggaa tcgccgctct
     4981 cgcgttccca cgtcgtggtt tcgccctgca atccgctttc ggtccaatcg aaacgaccac
     5041 aatcagcttt gggcaccggt cccatccctg ggatttgggt tgcactggcc gccagcgcgc
     5101 tgctgctgaa tgcgagaatc gccaaactga ctgcggcgca tgctgtgtgc actacgcgcc
     5161 tttgcgtaat gcataataga tggtgcttca ttttatttac tcctttccaa gttttttgaa
     5221 cgacgtaact ctgggtagcg cccttgataa ccaacgcctc tataatcgat cgacgttctc
     5281 taagagttgc ttcttcgtcg cgcagcttga ggcggtttcg aacgggtcag cggacgcatt
     5341 ctcagcaggt gagcggcccc ggcgtggaag gacaatcgtc gttctttaat ccctccagct
     5401 tgccgctgac gaattctcct tcgtagcgtg cacccttggc actcacgtaa atccctggac
     5461 catgggcttt gccctcgttg aagttaccct ccagatgggc gccactctgc caagtataga
     5521 tgcctttgcc gtcgggtcta cctttggcaa agtcaccggc atacttgtcg gctcctttcg
     5581 cttttcccat cccttgcgct agctcggtgc gttcgtcgca atcaccttca taagcaccgc
     5641 gaaggtaccc atcgcgcact cggcagtcgt gcgacatcgc cggcgctcct agtgtcatca
     5701 gaaaagcgcc gcagaagaac gcagatgaga gtctcttcat gttaagctcg gttgcgtgcg
     5761 aacggcagtt caccatgaat gcacgaagcc gctcgcatgt tgaaataaag caaagcaggg
     5821 cacacactct ggcgtgccct gctcgtttac tgaattccgc tacttgcgtg gtcgtggccg
     5881 agggcgtgcc gggtagacga tgtcggccgc cttacccgtc agctgtaaaa tatcgaaccc
     5941 agctccgttc cggtcgacta tgtagataaa tccacgatcg tcgatttcga cgttgttggt
     6001 catgtagccg tcaggattgg tgttggcgtt agattcaggt acgtagaacg ccacttcgag
     6061 cggtgcatgc ggctcgcgga tgtcccaaat ccgtacgccc cctgtgaagt acgcaatcga
     6121 cgtcagcttg ccatacaacg gattccagaa gttctcttcg ctcgagtgaa cgccgaagcg
     6181 cgctccacgc ccgcaataat tgccgcgcgg atatttttcg ccttcaaaag gatcgacaga
     6241 catcgtcgag aggaccatgg ggccttgaaa gacattctgc tgcacgcgtg tgcctggcgg
     6301 cgcgaacttg gaattttcca cggtcacatc gacgacgaag ctccagtgcg gcgcttcgtc
     6361 acacgtatca gccgtcgatt ctgacgccaa cagtacgatg tcgcgcgtct ggaactcggt
     6421 aaaggcctgg tagctgggcg gtttcaagcc gaacaccgga aacgaggtgt ggcccccctg
     6481 gtccggcgac atgtaaagaa ttcctgctat tgggtcaatc aattcctgct ccgtcggagc
     6541 gtctgcgctg tccggctttg ctgggaccca agtgcctccg tatgccgcag ggagcagctt
     6601 cttccggtcc agaatctgta gcaccccatc gtcgccgacg ccccacgccg cgtagacacg
     6661 gttgccgatg atgtcgtcgg ctgttgcgcc acgggcgagc ctgttcgccg caaagggatg
     6721 ctcatgtgct gagatcggac catggagcga ggtggcgatt ggcccggtcg accctggttg
     6781 cccgccgact aaaccgaacg tgcggatgta atttggcgca gcggtcggat ttttccagtc
     6841 gacgatcacc atcgactgcg cctgacgcca tagcggcccg ctatccttgc tgcccggcat
     6901 gtaggcgatg ccggtgtcgc actcccacca gtgtttgtgc gtcgagcgaa tgcctcgcag
     6961 cgccgacgcg agcaccggct ttttgacatc ggttacgtcc cagacttcgt agccagacat
     7021 ttgggcggtg tttccctgga tgttgcgcat caaataaact ttgcccctta agtcctcgtt
     7081 caggtcggag cccaagcaca atcgcgccat ttgcgcctgc ccgccggcga ccgggaccgg
     7141 gatatgggac atctcccttg gatcgttggg attggtgaca tcgatgatca tcgttccgtt
     7201 cggctcatcc gcattgttgt tcagcgggtt cggcaagaaa gtaaaaccag gcctggctac
     7261 cggaatattg ctgtgcgttc ccgagaaagc gatccatctt ccatccgggt actcaataac
     7321 attcggctgg taccccggcc gcccttggag gtcagtgtgc cccacgcgtt gcatgttttt
     7381 ttgttcgccg ctttggctta ccccggccgc cattgcgccg ctgctgaatg ccagaatggc
     7441 caaagtggcc gcggataatg cggtgcgcag tacgcggctg tgcgtaatgc atgcatcttg
     7501 cttcattggt ttactccttt catcccagct tttttacacg atataaggcc cagtacttcc
     7561 tacctaacgt aatgctaaag ccttaacccc tttacgggat cagctggctt gtcctcccga
     7621 ccgctaaact ctactgctgt acggacgaaa cgcgccctct acgatcaaag cgcacagatc
     7681 gcgagtcgcc gtaggaccat acttcaaagc gtggcccgtt gccgggctcc tcctgaacag
     7741 tttccgttgg ttctccccaa atggctttga cttcgtcggt cgtcatccca acacccggct
     7801 ctgcgggagg aacaggcgat ttcttcgggg cgaagacatc ctgcggcacc gaagcaatcg
     7861 agtccgattc gcagggaaaa ttctgataca gagtctctcc cctttttccc acgcacttga
     7921 acatttcagc ggaggcgaac ggcgcgacga acaagagaga gaatagaacg catccggatt
     7981 tcaacattgc cagctacctt cgatatgttt taggatttcc gtaaagcaaa tcggtgaaat
     8041 cagcagcata tactttactc acggatcggg gttcttcatg ttagcctccc tttatttccg
     8101 aatatcggtt tttcacctgc tcctgccttc cccttccttt gtagtctcat tgcattagtt
     8161 gtcgttgtga gtgcccaaac tcaaatgtct tcatgctggt tcgataaaaa agcagctata
     8221 cgaaaatcag cgatgtgact aatagttttt tccaatgcct gctataaggt tttgtttatg
     8281 ggagattcct gggctcgtca gggtgcgccc gtcaatcggt cgattccagg atgcttctat
     8341 caagcgatgg ggcggtaatc agtggtcgaa atctgcgtag acattcacaa tggaggtagg
     8401 attttcgcgt tgttgagaat tactaagatc cgtgaaaacg gcgttagccc tcgggcaggt
     8461 tggagcgatt tataaacgat tgcggagtgt ggagcacgag gatcgcataa ttttatctcg
     8521 ccacaacggt gaacaaggat atgcgcgctg gcaaaatagg atcgcgacca acaaggtcgg
     8581 atcgacaacc ggagactatc tactcgtcaa tggaggataa cactgggtgc cgatgctgga
     8641 ctgtgactgt acagcggtta gggtcttctc ttcttgggct gcttcaaggt gattccagcc
     8701 agcgtgtact gagccgctgc aagtggttta ttcgtgctgc atcgagcaag gcaccaaata
     8761 agattgcgaa cggagtgctt gaatctctgc gctgtcatcc catgtatcac ccgcgcgatc
     8821 aaagaaccga gagttattcg actcccgtga agttctgaga gcaaaacgca ggaggcgagt
     8881 ataagcttag tcgtcgtatg gcacggccac caggtgatcc tttgccgatg tagcgtcctc
     8941 attccacatt tccagcagct tcttggcgac cggtccgggt ttgccgtcgc cgattttctg
     9001 tccgtcccac tcgattaccg gggcgatttt gatcgagctg ccgaccagca tcatttctgc
     9061 cgcttgaagt ccttcctgca ccggaatagc ggcgagatcg atagcgctta atacgtcttg
     9121 ctgcacgagg cgcatggcga actcgagaac tctttggatt gtgatgcccg aaagaatcgc
     9181 ttcaaagggc ggatggcgga acacccgatc ccgcgtcacg aaagctacgt tcatattcga
     9241 gctttccgct accatgccgc gctcgtcgat gaaaattccg ttgtcggcgc cttgctcttt
     9301 ggcttccaat aggacgagga cgttaggcag ataattggtc gacttgatgc gggcgaaaag
     9361 cggcggcttg atcggcaccc ggctggtcac aagcttgatt ccgtcatgat agtagctttc
     9421 cgggtaagct tcttgtttaa agacgatcac atagaaactg ctgccgacgc actctgccgg
     9481 acccaaacca tagccgcctg gaccggcgga aagccaatag cgaaccgatc catcgcgctg
     9541 gcggctggcc gcagcagtgt cgagaataat ttgccgcagc tcctctcgag aaaacggcaa
     9601 cggaatacgt gctccgtgcg ccgatcgaat caaccggtcc agatgcgggt cgagttgata
     9661 cagcatcccg tgcgtcagtg tggcggtatc gaagacgccg tggccgcgat gcaccatatg
     9721 gtcgtcgagc ggcagtacca tgatagccgg atcgttgacg atgccaccga ggatgctcga
     9781 gtacatcgca taaaagttgt cggccttgcc gtgcactgtt tcgtgcaggc gggtgatcgc
     9841 ttgttcgcgg ttgaaaatag gagtcctagc catgattaat tcacctcgta atcacaagtc
     9901 gcggattgta aggatctcgc cagaatagtc acatctcatt ctactataga aacgaagaga
     9961 agaggctcac gttgagctct gcgactacgg aactgtcgtt tgcggcgcaa tgcaattcgt
    10021 gatttcgcgc gtgcaacttg ttatcgcacc gtgaagagcg cgaagggagt caaaccgaaa
    10081 gtgctgttcg tgcgctttgc gatcttctta gcgatgtcgt tgggcaataa ggttgtcgcg
    10141 ccctgaatta aaattacccc acgtgtacag aaaggccgca agcacccagt ggaagaaaat
    10201 cgggatcgta ccgccgaacc agcgcatgtc gggccggtag gtcatcgtca aaatgtaacg
    10261 caggatcatg ctagccgcgt agagaatgct gcaggcaacc aggaatcggc aacagccggg
    10321 tctcggcttg gcgaaaaagc ctcgacctcg ccgaatctcg gatgcaattt tgaccatcac
    10381 ggccagcata accaattgca cgccgagaag aataggatag gggatcagac cggagaacca
    10441 gtgttctgag ggaggcagcc aatcaaccga aaaaaacacc accagagctt gacccaagac
    10501 gcgaagcgca aagagcagag tcagaatcgc gagcgccggg ccgtgcatgc cgatgttaat
    10561 ctggagttgt cgatcgcgct gcgcctgcgc tttcaaccac gaactgtatt tcgtccggat
    10621 cttccggcgt gacgcacaaa agttgcgctt tgaattgcgt gtcgcggccg ccgcgctgca
    10681 gaattatttg ggccttgcgc tcgaaccaac cgggtttcat cacgcacaag accggagcac
    10741 cgagccgccg cgactcgcgc ttttgcgcat attcgatatt gagttctcgc aggcccgagt
    10801 caagttcttg cagcagctgc accagggttt ccgtgcttgg cgcgacgccg tccagttcga
    10861 cggagaaaac gtacagatac ttttcaacat cggcgcaggc gcgaaaatgt tgcaccgcca
    10921 cgccggcggc tctctgcgcc cgcgccatgg cgtcgatgat atggttgacg tgcaactttt
    10981 cgccggtgat gctggtgaca tcgcggcctt tgcggacgaa gtcgatgagc ggcgtttggt
    11041 tgtaaaatcc aacgacccta actacgtcat tgatgtcgta gcggtacaat ccgcccggcg
    11101 tcgtaagaat aatataataa agatttccct cctcgagctc tgcgcatgtc aaaatcgtcg
    11161 gcgcacccga attgatttcg gattccggga tgaactcgta gaagttctta tcgattgtga
    11221 gaatgccggc tgaccccgag tcgaataccg gcaaggtcat ctgcgcctcg ctggccatgt
    11281 aaccgagatc gcgcacgggc gtcgttttgg caaaccagcc ggcgaattct ttcagtcgca
    11341 caccggcgct gccgcctttc cagcaaccga taagttgcag ccgcggccaa tattctttag
    11401 gccggagcgc tccgtcgtgc ttgatcaaag attcgagtcg gcgcgcgcgc aactggtttt
    11461 tccccagccg tgatgcgagt gatgcgcgca gttgggccgg aagatcgaaa cgggagctaa
    11521 tagtcccgtc atggatgtct ttgatgatgt cgtatttatt ttgactggcg ctttggacta
    11581 gcttgagaat cgtactcgga ttgggcgtgc cgaaaaacga gatatcctgt tccagcgcca
    11641 gacgcatgat caaatagtat ttggcttcga agtctttgat ctcgcaggcg tcgtacggca
    11701 cggcgtaagc gttctttatc caacgcgggc tgcttcgata aatcaaaccc gaggcggccc
    11761 cgaatggaat gccgctggtt gtttttccct cgacggccgg gctgatcacg ccgagcagct
    11821 tgccgttgaa gagatcggca tggtccacga gcgcgcggta ataccacagc cgcgtcaatt
    11881 gccggtggtt ctgtcgcgtt tttgccgtga ccgggatcag cttgggctca cccgtgctgc
    11941 cgctggtcat cgtgaacatg tcgacgggct ccgcggtcag cgcgtcgcgc tcgccgttgt
    12001 tgatgagctc gacataggga cgcaggcgct cgtagtcggc gatcgccact tgatgacgat
    12061 aatcgctcag agaacgcatc gatttgaaac ggtgatcgtg gccgaagcgc gtggcgcgat
    12121 tgcgcgcgag gatctcgaga agcagttcgt cctgggtttc ttggggtttg gctgtaagtt
    12181 tgcgccagcg tgtccactgg cgccgggtta cgaaggcagc ggctttgacg agcagtcgca
    12241 ttgatgatta ccggtgcaga tcgatcttcg aggcagcgag gcgttccaag acttcttcct
    12301 ttactgtgac gctaaggccg cggttttgca aaagcggcgc gcgcccgccc aagccaaacg
    12361 cgatgttttc aaaggtgaca tcctcagcga gcagccaggt accgaacgag ccttcggcaa
    12421 aggccagcgc gggcaaatgc gccgcgaaga ttcgtcctgc ggcggacaaa atgccggtct
    12481 cgcccacctg tgcgcctact tgaatcttga tcccggcttc tcgcgccact gtggcgattg
    12541 ccaggctgcc ggcgataccg ccgtttttcg agagcctgat gttgaaataa tcgcaagcgc
    12601 cgagttctat taggcggcgc ccctgatcca aggtgaccag cgactcatcg gccataaccg
    12661 ggacgccgct ctcctgacgt actcgtctca ttcccatcag gtcgtcggcg cgcaccggct
    12721 gttcgatcgc ctgcaagtta aagcgcgcca gccgttgtag ttgttggata gcctcgtccg
    12781 gcgcccacgc gccgttggcg tcggcgcgca attcaacgtt gccaccgacg gctttacgca
    12841 cggcttcgag ccgggccgcg tcatcagcgg tgccgagttt cactttcatt tggcaaatgc
    12901 cgattcgggc cattctcgtg gcgagagcgg ctgcgtcgtc tggcgcatcg gccgagatca
    12961 cgccgctgta gacgacttcg tagcgcgcgg gcggcagcaa gtcggccaag gcgcagtgat
    13021 cggcgcgcag gctccagtcg agcaatgcca gttcgacggc gcagaaggcg gcgttccacg
    13081 ccgcaacgct gctttgcccc tgttcgggtt tggtccattc cggcatgacg gattgaagat
    13141 attccaagct ttcccatccg ggtgcgaacg attgcgaaaa tacccgaggc gccagtcgct
    13201 cgcgaatgcg tgcgagcatg gattcggttg tctcgccggt gacataggat cgtggcaagc
    13261 tctcgccgaa accgcggcgg ccgtcgctat cgatgatttt gacaattact gcatcgctct
    13321 cttcgcgact gtgtagcgaa tggcgaaacg tttggtggaa tgggatgcgc agccggtaga
    13381 tagtcatggc ctcgatacgc cgcgccctaa ccgggcgcga gcgagcgccg gtgcgctgtt
    13441 tcgagccggc tggaaagtag tggcgagcga agaggttctc gcccaacgta agactcgcga
    13501 tttgttcgaa gagatcgtcg gatgcttggt gaaagcgcag atagtcctgg atgacggtta
    13561 cgccttcttc ggcgtgacgc acgtcgactt ccgaatggag cgcaaaccag tgcaacgcag
    13621 ttttagcaaa gccatagtgg ctggtcaggg cggcggctat ctcgctcgca cagcgcgaca
    13681 gcatgaactc aaaactcatg gtctcgatca gcaccgccag gcaaagctcc cgcagggtca
    13741 aggccgggat gcgagcggcg cagaagaccg ccagccggcg gcgcgcatcg tcgatcagcc
    13801 ggtcgatctc actctggtca aaaccgacgc cgcgcgccag ttccaggagc agggccgaat
    13861 gcggcttttc aagatcggag cggcccattt cttccaatag attttctctc agcaactccg
    13921 ccgagctcga cggcaatgac gcgaagcaca aggcaacaat atgggaggag agaaagtgcg
    13981 tgcgaaaaac attgcgtaga aactcgcgcg cagcctctag gttagcccgt cccgccacca
    14041 gctcttggta ttcatcggat tggcggaatt gccgggttgc gctttcgaac gtctgggtga
    14101 ttttttcggg ggaaatcatc ttgctatagt aggggaccgg ctattcccgc ttaaaaccca
    14161 tctcacaatc tgcgggccga agattcagca aactccaatc cggccgactc actgggggtt
    14221 tagaatcgcg cgtctctgat taggagcagt cgataaaagt atacttgcgg cctttgacgc
    14281 agtcgagaag attcggcgcg gtcagccctt gactgatatc agccgaagcg ggtcggcaat
    14341 gcggctaacg acgtatcgag gggtggcgca ctacgcttaa ggacgctccg cggtttgcag
    14401 aggcttggtg taaagtttga agccgccgaa tccatactcg cctaccggtt tgttggtggc
    14461 ggcatcaaaa agatcgataa gcataaattt ttccggatgt cctgcaagaa agttcgaaaa
    14521 cgtatcgaca aaacgctctt ggctttgaac gtcggcgcga taaaagcctt cacccaggta
    14581 caatcgatgg gggcgtggtc gggcatcgag acgggtccaa tattgagagc cgttagatcc
    14641 cagcgaatcg agccatgttt tatactgagc gatttttgct tcccgctctt tgaactcgtt
    14701 ggcgggcacg tccgaaggtg cagaaattcc ggctacaaag acgatgagaa aagcggcgac
    14761 aaatgctttc atgatttcct ccgatcacta ttccgggtat ttaacgcaag gcatttgcca
    14821 tcatcagccg gatcccacga tagtggcatc tccgggcgaa cgcgtgcgca atcgtgctct
    14881 tctgtgccaa catggcaact caaggacggt gatgtgcgtt agggggggag attgtgattg
    14941 gcgtcgatga ggcgatgcgg cgatctctta tcgcacaata tcgctcgaga gcgacgaaag
    15001 tgcgtttcac ttccgtcagc tcttcagccg ataccctgtc ttaaagatcc atgcgactac
    15061 cgccaggcag attgcaaaaa accccagggt catcgccagg ctcacaccga cactcacgtc
    15121 cgaggtgccg taaaagctcc agcgaaatcc gctgatgaga tagacgaccg gattgaacaa
    15181 cgtgaccgtg cgccatgccg gcggcagcat gtcgatcgaa tagaaggcgc cgccgagaaa
    15241 ggtcaggggc gttatgacca gcatcgggat gaactgcaac tgctcgaagc ctttggccca
    15301 gaggccgatg atgaaaccga aaagactgaa ggttgccgcc gtcagcacca aaaaaccgat
    15361 catccacaaa ggatggagga tcgtgatcgg cacgaacaac gcggctgtcg acaggatgat
    15421 aaggccgaga acgatggatt tggtcgctgc ggcaccgacg taggcaagga caatttccat
    15481 ggaggatacc ggggcggaca agagttcata aaccgtcccg gtaaacttcg gaaaataaat
    15541 cccgaaggag gcattggaga tgctctgggt caacagcgac agcatcatca agcccggcac
    15601 gatgaacgag ccgtaggcga cgccgttgac ctcggtcatg cgcgagccga tagcggcgcc
    15661 gaagaccacg aagtaaagcg aggtggtcat aaccggcgtt acgacgcttt gcagcagtgt
    15721 gcgcagggcg cgcgccatct caaacttgta gatcgcccag acaccgtgcc tattaaatgc
    15781 cgatggttgg gtcgccgtgc tcatttgcga tcgcttacca ggctgacgaa gatgtcttcg
    15841 agcgagctct gccgggtgtt gaggtccttg aagccgatac cgaggtcgcc cagccctttg
    15901 agtaacattg gtattcccgt gctctcgtca tcgccgtgga aggtgtattc cagctcgcga
    15961 ccgccgtctt tcagcgccag cggccattgg cttagctcgg ctggcagcga cggtaaaggc
    16021 tcgagaagac tcaaagtgag ctgcttcgtg ccgagttttt tcatcagctc gactttttct
    16081 tcaaccacga tcagcgctcc tttgttgatc acgccgatgc ggtcggccat ctcctcggcc
    16141 tcatcgatgt agtgggtggt caggatgatg gtcacaccgg tttcgcgcag ggttcgcacc
    16201 agcgcccaca tatcccggcg cagctcaacg tccacgcctg cggtgggctc atcgagaaaa
    16261 agaatctccg gctcgtgcgc cagcgccttg gcgatcagca cgcgccgctt catgccgccc
    16321 gacaaggtca ttatcttgtt tttgcgttta tcccataaag acaaatcccg cagcaccttc
    16381 tcgatatgag ccgggtccgg ggcatgaccg aagagtcccc ggctgaagct caccgtcgcc
    16441 cagacggact cgaacgcgtc agtggtcagt tcctgcggca ccagaccgat cttcgatcgc
    16501 gccgcgcggt attcgccgat gatgtcgtga ccgtctacga gcacgatccc cgccgtcggg
    16561 gtaacgatac cgcagacgat gctgatcaag gtagttttgc cggcgccgtt gggacccaat
    16621 agagcaaaga tttcaccctt gcggatctcg aggtcgatac ccttcagcgc ctgataaccg
    16681 gaagcgtagg tcttggtcaa gttcgaaatg gaaatgactg gcggcatagg ggatcttaat
    16741 gaggggcatg ttgacgcgcc gtcgatatac cggacggtgg tcaagcaggt tttgcactag
    16801 tagtcaccac cagaatgacg gcctgtgctc tggagggata gcgatttcaa gcgattcaag
    16861 attctctgcc tgaagattac cagattcaaa gccacatcaa agccgtgtcg gtcttcgcga
    16921 ggattgccga cgaaagtttt ttcttgccta gtacaatgtc tcacgctttt gggtacttta
    16981 cgggtgtata ccgccacgga gaagtcgaag gacacgaaga aaaagaaagc atattagatc
    17041 ctggttcgtg tttcgattgt ttgtttttgt gtcgtttgag cgcttcgtgg tgaacagaaa
    17101 cattttgctc gacggccctg tcgagagccc aggatcaggt tgtgcggctg agttgccttc
    17161 tgagatactg gcccgtggaa gcttcggtga ccctggcacg aggagcgccc tcaggccatt
    17221 ccatcacaat gttgccgcgg accacagtca tgaccggcca gccggtaaca tcccagccct
    17281 cgtataccga ccaaggggtg acggtgtgga gaagttcttt agtgatcttt gcacgccgct
    17341 tcaaatcgac gaccaccagg tcggcatccg cgccgggaag gatcgcgcct tttttcggat
    17401 agaggccaaa gatgcgcgct ggattttcac tggcgacttg ggccagccgc tcgatggaga
    17461 ttcgaccctt attgacgccc tcgctcagca tcatcggcag catcccttcg acccgcgaag
    17521 gaaagccgct gatggtggtc caaacgtttc cggtctccat cgattcgcgg gttctggtgt
    17581 gggcgacatg atcgctgccg atgcaatcga tggcgccgga cgagagcgcc ttccataggc
    17641 tttcgtgcgt ggagcgatcg cgcaacggca cgttgatttt ccacgcgtcc ttgtgcagca
    17701 cgagatagtg ggtgccgatt tcactataga ccgtcaggcc ttcgactttg gctctctcga
    17761 tttccgcaac ggtctcggtt gtcgtacagt gcaccacgta aatcggacag ccggcgatcc
    17821 gggcataata aaggtacgtc cgggcgtgac cgacctcggt gaaagccggc gaacgatcat
    17881 cccacgcccc catgtcgttg cgcccggcag cgatgagctg cttctgcagc acctgggcga
    17941 tctcccaatt ctcgcaatga agcagcgcga ttgccggcgc gccgagcttg gcgaccgctt
    18001 taaaaaccga gtaaatgctg ccgtcatcaa aaaagtaagc gggccacgag gacttccatg
    18061 gtccctgcat ttgcaagtga agcttaaacg agctgatgcc gtgctcatga gcgagataag
    18121 ggatttcgtt gacgtgctcg tcggtactga cgatcggcgt tagaaaaaaa tcgatggccg
    18181 cgctttttgc ggcttcatag aaaatcggca tcgacttcgt gaaattggga atatctcccg
    18241 gtcccttcac tttatccgcg ccgccggtaa ttgccaggct tacaagctgc aaaccccagg
    18301 tcgtcacacc ggtagccgca gccgctcggg tctcgctttt aaaatcggcg gccaggtcgc
    18361 gatggccgcc gaggtgacac tcgggatcga tcactcccgg aagaagatgt ttgccggcca
    18421 agtcgatgat ctgctctgca tccggcaaaa acgcatcgcc gccgacaaca gcgatctttc
    18481 cgtcgacgat tccgacgccg ccttcgatga caccggaagg cgtgacgatc aggccgttct
    18541 taaaaagaat atctgctgtg atcttagcca taacttactt cgagaagaat tgcttttccg
    18601 agtcgatgat cgatttcgcc cagcgcgaaa ccagggtttc ggcgaatgag cgttcgtgga
    18661 tccatttctg gctcgggccc ataatcgagt ctttgtacca gcgcggcact acgcgcaccg
    18721 caagcatgta gcgcataaga tttaccacca gctcgttgac gtttttttcc accgggaagt
    18781 catcccaccc gaacgcgagc ccggtgaagg gatccaccag acccatcgtt gcgcccgcct
    18841 cgtgaacgcg ccgaccgacg acctcgctat gaaggatctc cggccgctcg agcccggcgg
    18901 ccaaacaggc ttcgatggcg aagccgccgt agttcgactt ggaaccgaat acgcagtaat
    18961 cggcggcggt tgcggcggcg atgccggact cgcacgggca gccgcattgg gcgccggtgg
    19021 gtacaactct ccgcacagcc tcggcgatgg aagcagtgcc gatttcgttg cccccgtcac
    19081 cgacgcctat ggtcggtatg cctcgatcgc cggcgaaatc aaaaaggtaa tccacttttg
    19141 cttgcaccga cgacacgtcg atgccgaacc cgttgtgata aatgcctttg gcgttcttcg
    19201 acaacttttc tatcgccacg acggctttcg gtttgaactg atccagaagc cgaatcgcct
    19261 cgcgccgtcc caagtcgtcg tcaagaggaa acggcagaac gatgactttt ctcggcgttc
    19321 ccaccgccag ttcgggcgcg ctcggcatca ggccggcggc ctgacaaaga gcctccatcg
    19381 ctttcaccac gactccttcg caaacgatca ctggagtcgc gccgaggccg acgtcaatcg
    19441 cccgcgccaa ggtcgcggcg gcaatcgggc cgtcgccttc ggcgatcatg tagggcggtg
    19501 caatcaggcc ggtggtgatc agcacggtgt cgccgcgctc gacgttgtcg gcgagcgctt
    19561 tggccgcaag atgacagagc ggcgcgccgg ccttttcacg ggccgcttcg taaagcacgc
    19621 cggttaccgg tttccgcgtc agcgccgagc cgccacccga catgaaatac tgcaccgaga
    19681 tcaggcgatc gacggtgtcg cccagattgt acgaaagctc ttcatttttc acgtggccgg
    19741 tttccttatt ttcttgcttc ctgcagatag ctcatatcga ttacgccatc gacggggggc
    19801 ggaggttttt gcaagccgaa ctccttccag ctttcggcga cgatgcgggc gcccgtgatg
    19861 tcgatcccac cgtcgcgggg aattgatttc ttggtcttga taaaaagact ataggtcttt
    19921 tcggcctcat cacgctccgc ctttgccttc tcgatcaacg cccgcaacac ggcctcttta
    19981 tttttcggct cgtagaagaa gtcgcaggct cgcacgaaag cgcgaaggaa tcgcaccatc
    20041 aacggtctgt tggcctgcgc ccagtcgcgt cgagcgcccg tcacggtgta ttgaaagttg
    20101 tcgacataat cagacgagta accaagcaag cgaagtcccg atgcgatggc tttgaaatcc
    20161 gatggctggt tgaccaacgc tccggcgacg ccgccttttt ccaccgctgc cagacgggtc
    20221 ggagttccgc ctaatccgat catctcgtaa tcggcaggcg tcaatccatt ggccgccagc
    20281 atgcgtgcca gcacataatt cagcggatcg gttggactcg cgacggctag acgcttgcct
    20341 ttcaagtcgg catagctgtt gattgaagga tgcacgatca gtgcatagat aggcacccag
    20401 gtatccccgg caacggctat gagatcggcg ccggcggagt tggcggccag caattgcacc
    20461 agattcattt gggcgagatc ggtggatccc ccgaccaagg cctgggaggt agccgtagtg
    20521 gtgccgccca ttacccgctt tatctcgatg ccctccttct gaagcatgcc ttcctgctct
    20581 gcaatcagat agggccagtg caacgccgat atggtgatgg tgccgtagtt gatcggcgtc
    20641 agctttgggc tttgctgcgc gtcggtgcga gttgccgagt ttatcaacac gagcgcagca
    20701 aaccagaaaa tatactttag agcggatgaa gtctttgtca tagtttgcac cttgagagaa
    20761 cagctctcga ttaagaaatc gtcacacgtt tctcataaga cgatggccgg ttacgtacta
    20821 tgagcccgag agcctgtcaa accaaagtta gaagagctga ccgtatgatg tcaaacatga
    20881 tcagggatgc ggcccgggcc acgacctggc tgaactcact cccaagtgaa tcggtaacgg
    20941 gtgttgcagc cgacaccttc gctgtacccg tgccccacgc gatcatctgg gatcacgcct
    21001 tgattgctcg ctggctttgc gggaaaatcc ctctgcgtta ggtcgtgcgc ccatgataca
    21061 ttctgagcta tcgttgcgtt tggcgcgccc ggcagaggcc gagagcattg ccactctatc
    21121 acgcgatttg atcgaatacg gtctcgattg gcgctggacg cctgtgcgag tcgcggcgag
    21181 tattcgcgct gccaacgtca acgttctcgt cgcatcgatg cacgaggaca ttgccggttt
    21241 tgccatcatg cgctacggcg acgacgatgc gcacttggac cttctcgccg tatctccctc
    21301 ccatagacga accgggatag ggcgccaact gcttgagtgg ttggaagaat gcgccgtcgt
    21361 ggcgggaata ttcaacgtcg atctcgaagt gcgcgcaggc aatgaagaag cccagctctt
    21421 ttacactcga atgggttacc gcccgctgct tcaacttccc ggctactatc aaggtgtcga
    21481 ggcagcgctt cgcatgggcc gcgatttatc gcgccgatct ctcgaccaaa gactcacggg
    21541 aagcaatcct aaaagctgct gaaaagcctt ataatcattg tggcgctcag cagtttcggt
    21601 caatgattgc ggcagcgggc atgagcaaac ttacttcatc aagcgcatcc gaggtttgca
    21661 ggcaactcgc cgccaacagc ctaaggatcg gatgattatg agcacaaagt taataacagg
    21721 tgacggtttc acaaaaatgc cgggcttggc aaccgagcgt gatcaaggga gccgcacgtg
    21781 actcgtcggg gtgaagagta tgtgcgcggc ttgcatgacg gacgcacggt gctgttgaac
    21841 ggcgaacgag tgcccaatgt gaccacacat ccggcctttg ccgcggggat tcgcagcatc
    21901 gccaacctct acgatctcgc tgctgatcct gccaatcgcg agctgatgac ttatccgtcg
    21961 ccgcgcgacg gccgtccgac caacaagtcc tggctggtgc cgcgcagtcg cgatgacctg
    22021 gcggcgagga ggcgcgcaat taaattctgg gccgacgcca gttatggttt gctgggacgc
    22081 tcgcccgatc atgtcgcatc atttttcgcg ggatttgcag gtgcgcctga tttctacgct
    22141 cggggcgggc aacagctcgc cgacaatcta tcgcggttcg cagccaaggc cgcggatgag
    22201 gacctctatg tcagttatgt catcgtccat cccacggtcg atcgcagcaa accggcacac
    22261 cagcaggccg agccgtatct ctacgccggc gtcgccgccg agcgcgacaa cggcatcgtc
    22321 attcgcggcg cgcagatgct cggcacaggc tcggtgatgt ccgattatgt gctgcttacg
    22381 gtcatccttc cgctcaaacc cggcgatgaa gactacgcga tttcctgcgt ggtgccgaat
    22441 agcgcgtccg gtctgaagct ctacccgcgc cggccgtatg cgctgggagc caacagcata
    22501 ttcgactacc cgctatcgag ccgtttcgat gaaaccgatt ctctggtcgt cttcgacgat
    22561 gtcctggtgc catgggaaga cgtgtttatc tacaagaaca ttgagcttac cgcgggtcag
    22621 ttcagtgtta ccgccgcgca cgtcctggga aatacccagt cacacattcg ctcatgggca
    22681 aagttgcagt ttctcgtcgg attgatcaag cgctgcatgg atctgagcgg ccgctccgcc
    22741 aataccgaga tagttgcgca gctcggtgac ctcgccaccc gtgtttcgct catcgaaggc
    22801 atgatccttg gcgccgaagc ggcggcggag ccggacgcgt ttggtgtcat gcgccccaaa
    22861 gattccctgc tttatgccag ccaggtgttc cagcaggcga tgtatccggc gttgctcaac
    22921 cagattcgcg gcctgatggg cggcagcctg atccagttac cggcaaccgt ggctgagcta
    22981 cgcgcgacgc cttcatcggg ggatatcgac cgctacatac gctggccgaa agccggcgca
    23041 cgcgatcggg tgaagctgct gaagcttctg tgggatgccg ttggttcgga gtttggctcg
    23101 cgtcatttgc agtacgagat gttttacgcc ggcgagcccg cggcgattca gggacgcgag
    23161 tttcgaacct tcgattgggc ggccgcggag gcattggtcg atcgttgtct ggcgacctac
    23221 gatgagagca cagcttagcg gcgttataga aatttctctc gatgacagca cacaaggtcc
    23281 ttcgcttcca aacgccgaac gatactctcc taagcttcat cgatagcaaa gccgccttta
    23341 ccgatttctg aaatcgaacg gcgattgctc gatcccgtac gacagtgagg cgcttggttc
    23401 tcagcgcgcc cacccattaa ggcatctcag tgcctttatc gtggcatgcg ccttgcgcct
    23461 gtagcaggaa acagtacgtg taaaggaggc ccatatgaaa cggaaaaagc atattctaac
    23521 agcggttttt atatccggta ctatcggcgc gggcgtgaac ggattgttcg cccagtctat
    23581 tcctggacca accgtcccaa aaccggtacc cgaagtcccg cagcaaacac agccgacgct
    23641 accgggccag ccggcgccag gtcttccacc gacggagcct cttccaggac agcgagggac
    23701 tatacccgaa cggatgcaag caccggatgc gggcagccag cgcgatatgg tgatctcctc
    23761 ggaagacatc aaaaaggctc aggaagcatt gaaggcgaag gggaaagacc caggggcagt
    23821 cagcggccgt atgcatgcca agacccaaga agcgcttcgt gagtttcaga aagaaaataa
    23881 tttaccagct accggagtac tcgaccagaa aaccgccgag aagctgggtg tgaccttgaa
    23941 gggtgatgaa ggttcgaagc cgcagcaacg gcaacaaggt gccaagccag aggctaagta
    24001 aatttatatt tagggttctc acaatacaga tcgggaagac gcaatacggt cttcccgatc
    24061 tgcaaccgca tcatccttcg cgcttccacc taacccatta ctcgttacga ttgcagtcgc
    24121 ctgtgctgtt ctgcagcgga ataggcgaag gtgcattcat ctcgtcccgc ttttgaaggg
    24181 ccagttgagc caagcggcga tgccgcggcc catgatccat tccttatctg tcgccgaaag
    24241 gaaaccgagc tcttccgtga acagggtcac tgcttgtcga taggagcacg gtaaatgaga
    24301 aagatctgtg ccccaaaaac agcgttgagg gccgaaggcg tcgacaacgc gatgaattag
    24361 cgggtgaaga gtcggaaacg ggtaagcttc ctcaacataa cagggcaatg cagaaacttt
    24421 taccgcgaca ttaggcaagc gcgccatctt aatcagatca tccacggctg aatccaacgg
    24481 cttaccgcgc aatgcggtgc tcagtcccat atgatcgatg atcatgcgca accctgaatg
    24541 actttcggcg atggcggcga gtttcggtac tgcgccgggc gcaaaggcca ttactgggat
    24601 atcgtagcgc tgcgcggcag gccaaaacca atccgcggtg ccgtcatcca gccactctct
    24661 cgagcgtccg cgattgaaaa ccattctaat gccaagcatg tgcggctggt ctttccaagt
    24721 cgccagcaag gcgcggcttt ccggcgcgtt tattttaagc cgtcccatga cgccaaaacg
    24781 gtcgggatgc gaacgcgccg cttccaacga ggtgtcgttg cgatccgctt cccaggtagg
    24841 cggcacgagc acgcaccgat tcactccagc accgtccatt tcccccaaca gttcctcatg
    24901 cccaagcggt atgaggcgat gcggttggga tgcgttgcct ttggcatagg gcttctccgg
    24961 cgtttcctcc gcccaaatat ggacctgagc atcaatgatc atcatatcgg ctgacctagt
    25021 tcaaaatttt taaagacgat taatcgttca atgccggatt catagcttcg gtcttgtgcc
    25081 gccgtcaaca acaaaagatt tctcctgcct cggaaatgac agaaggattt gtccgtttta
    25141 acttcgagag aacgcagcaa tgaagtaatc ttctttggat cggtggtcgg aagatccaga
    25201 ccgatttcgt tcagcgcacc ttcggattca acttttgctg aaatctattt gcttgaataa
    25261 tttttccgcg gcttcttcgc cggtgtgcac gcagtcggcg ataccgacac cgtgatacgc
    25321 gctgcctgcc agcgccagat tggcgtattt cccgagcgcc gactcgatcc gttgaattct
    25381 tgcttgatgg ccgacatgat attgcggcat agcgttcggg tggcgctgga tgcggaccaa
    25441 cagcggttcg gcgttgacgc cgagcaggtt gccgatctcg gctcgaacgt ttcgttccag
    25501 gatcgcatcg ttgtcatgca aaaaatccgg ttgcaggctg ccaccaacga aggcgcgcaa
    25561 tagcaaatgg ccttccgggg ccctaccggg atatttcaaa ctactgaaag tgcaagccat
    25621 cattttgcgt gattcgatcg ccggtacgac aaagccgaag ctgtcgggga ttcgtggaaa
    25681 gtcccggtta cgatatgcca gacttatggt tgcgcttgag gcgtaagtga tacccgataa
    25741 ctctttggct gcttcgggaa cggccgaagc caaaatccgt gccgtgtgaa aggcgggagc
    25801 ggccaaaatg atgccgtcgg cctcgagaat ttcgccggtc ccggttgcga tctgccattt
    25861 gtttttgcca tcgctgggaa ataggcttct tacagcagta ttgaggcgag ggcttgcttg
    25921 cggcagacgt tgggcaatca catcgatcat ttcctgcatg ccgccggcaa gggtaacgaa
    25981 taggctccaa cgcgcgccgc tgccgccctc gcgcttgcgc gcacggcgcc gctgttccga
    26041 ccacattgcc caaatgacgc tgcgttgaga tttttccatc tgtttgaagc gcggcatggt
    26101 ggagatgagg ctgagcttgt cgggatcggc ggcgtaaatt ccgccaagca gcggctgggc
    26161 gacccgttcc aaggcttcgt tgccaaaacg acgccggata aaggcgccga gactttcatc
    26221 catgttggca tcacctcgcg gcatcagcag ttccgccgcc atgcgcagtt tcccgcgcca
    26281 tgaaaacagc ggtgttcgaa tgaagggagc cagttgggtc ggcgccagca agaaaaaacc
    26341 ttccggcagc ggcacgagct tgccgttgtg aacgacgtag atcttctgat gcgcagcctg
    26401 ggtcgacacc aaacggggtg ccagtccgag ccggtcacaa agtcgcagcg cccagggttt
    26461 ttcagagatg aacgaatccg gtccggcctc gatgagaaaa tcgccaaccc gctctgtcgc
    26521 tatgcagccg ccgaggcgat gggacgcctc aagcagaaga atttcgaggt ctgaggcttg
    26581 ggcgtttcgt aactcgatca ggcggtgcgc cgccgccaat ccggcgatgc cgccaccaac
    26641 gatggcgatg cgtcggctca tgagcggcgc ctctctcttc gattgcggga cgagggagtc
    26701 aatcgttttg aaatttgcaa cttactgact ggcctggacg gatttggcaa tcacggccgc
    26761 gatcatttta atgaaggttg tgtgatcatt cgggcagctg gcgcggtgta actcgatgcc
    26821 ggcctgtgct gctgttttct gcgcttcgat gtccaaatcg taaagaacct cgacgtggtc
    26881 gcaaacaaag ccgatgggag cgatgacgac attgctagcc cccttaaccg agagatcgcg
    26941 tatgaccgag ttgatgtcgg gctcgagcca ggcatcggcc gggtttccac tgcggctctg
    27001 ataggcgatg gaccagttct tgtgatcgat cttttcagcg atggcgcgcg cagtgattgc
    27061 aagctgttca acgtacggcg atcggtcagc catcgacacc ggaacgctgt gcgcggtgaa
    27121 aactagggga gtcgccggac gttgctcacg cgcaatcccg gcaaagcttt ctctgattag
    27181 ctcgcaccag gtctggataa acagcggatc atcgtgccat cgggcgcagt agtcgatctc
    27241 cggcgcggcg cggccgagtt ccaaacgcgc atcggcaacg ttcctttgat acctctccca
    27301 actcgcatcg gtgtggtgcg aggaaagaat aaatcccaat gcccgctcaa cgccgtctcc
    27361 ggccatttgt ctgagtgtat cggcgagaaa tggggcggca tttcgcatac cgatgtaaac
    27421 tcgcaaatcc atgccgtgtt cgctcagtag tctttgcagg gcgcgcgctt gccgtgcggt
    27481 gatttcattc aagggtgatt tgccgccgac cgcttcatag tggcgcgcca catcctcgag
    27541 acgctgcggc ggaatcgccg tgccttgcgt gacccgcgct aaaaacggcc gaatctcatc
    27601 cggagaagtt gggccgccga aagcgatcaa gagaacggct tgataggaaa ccatggaatg
    27661 aagattagcg gagcttctgc agctcaaagt gacatagcaa ttgagcggca accccttcgg
    27721 accgaaggca atgcgctacc ggtttgcagc ggcctgcaat ctccggcgct gcagtggact
    27781 ccgggaaaat tgccagccac tgtgccaagg accatggatg gcagtgaaat tgttcgcttc
    27841 tgtgccttcg ctcctccacg cttacctctt gaccgtacat aagggttcat ttcaactgcg
    27901 gatccccgct tagcgtgcgc tcaattcgtg aacggcgtcg accatggcga tgacatgatc
    27961 gaccggagtt tcgggcagca cgccgtgccc caggttgaag atatggccgg gccgattacc
    28021 ggcgcggtcg agcacatcgg cgacacggct cctaatttct ttcggcggtg caaacagagc
    28081 gacgggatcg agattaccct gcaccgcgac gtcatgtcct acggttgccc aaccttcgtc
    28141 cagattaatg cgccaatcga gaccgatcac gttgccgccc gcggcgcgaa tgtgcttcag
    28201 cagtccagcc gtgcccgtgc tgaaattgat cacaggcaca cccttggtaa tcccggcgat
    28261 gactgagcgt gtgtacggca gaacaaattg ttcgtaatcg ccgggtgtca ggcaacctgc
    28321 ccaactatcg aaaagttgca cggcttgaac gccggcggca atctgacaat tgagatattt
    28381 cgtgatcagc tgcgaaagcc gctgcatcag ccggtgccaa acctccggag ccgtatagta
    28441 caactttttt gcatgtatat agttacgcga gccggccccc tcgattagat aggacgccag
    28501 agtaaaaggc gctccggcaa acccgatcag cggaacccga tcatccagcg cggcgcaagt
    28561 tttctttacg gcttcgtaga cgaacgaaag ctccttctca ggatcgaagt ctttaagacc
    28621 gtcaacatct ttgccgctgc gcaccggccg gtggatcaca ggaccatcgc ctttggaata
    28681 ctccagtccc acacccatgg gctcgaggat caacaatata tcggcgaaaa ggatcgccgc
    28741 gtctacgttg agtcgctcga cgggggtgac ggtgatttcg gccgcgagat cgggtttttt
    28801 gcatagctca agaaaaccgt actgcgccct cagagtgcga tagtcttcca tgtaacggcc
    28861 cgcctgacgc atgagccaga ccggcgtatg aggcactgct tcgcgccggc atgcggtaag
    28921 gaacggatga ttttgaattg atcgattgcg catggacaga agactacacg agactacgga
    28981 gcttcggcgg tgaaatcaac tgcggggttt acgcggcccg gccgcgccga tggcatcatc
    29041 accgtactta tcgcgcagat gatcgatgcc gtgatagagc ttgtcccacg agctgcgctt
    29101 gtcccggtcc agaagagact cctgccagcc ggaggattgc agcgcgctgg cgctgacgcc
    29161 caggagccga acggccctgt cctgagcaag attctgctgc agcagttgct tgacggtttc
    29221 gaagatctcc cgatcgtagc aggttggcga attcaaggtc cgagagcgag taatcgtctg
    29281 gaaatcgctg taacggatct taaccgtaag acatcgggcg taatagtttt ctttgcgcaa
    29341 cctgcagcca acctcctcga caagttgcca gataatccgt tccagctgct cgaagccctg
    29401 cagcggctcg aacgtagtct cgttgccgat cgaatgctcg tgagaacgct ggcgatgctc
    29461 gacttggccg agatcgaggt gacgtgaacc aagctttgaa ttcatcaagt cgccgatggt
    29521 tcggatgcct cgagctagta actccttctg tgttttcgga cctacgcccg gaaccagctc
    29581 aaccggtagc gtcgcgagaa agtttgtctc atcgccgcca gcaacaaaaa tgagcccctg
    29641 aggtttggct agagtcgcgg cgattttgct gaccacccgg tggctggaaa ctccgcccga
    29701 tgacggcagc ccaagctcct gttcgatgcg ccggatgatt cgatcggcag tctgcaacgg
    29761 cggccccagg agtttctccg tgccggtaag atcgacgacg ccttcgtcaa tcgagatcgc
    29821 ctgcacttcg ggcgagtact gccgcaggat ggcaaatacc ttgtgggaat attccgaata
    29881 cagccgatgg cggttaggaa gaaatatccc gtgcgggcac agcttattag cttgaaaccc
    29941 cggcatcgcc gaacggacgc cgaattttcg cgcttc
//
LOCUS       DASUVS010000325         5880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_135483, whole genome shotgun sequence.
ACCESSION   DASUVS010000325 DASUVS010000000
VERSION     DASUVS010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135483"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VF182_17415"
     CDS             <1..466
                     /locus_tag="VF182_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX6438919.1"
                     /translation="LIKTHFPADPERAGIFGHSMGGHGAITIALKNPKLYRSISAFAP
                     IAAPKQCPWGEKAFSNYLGAQRDSWDEYDSTEILLKLQDAPRRTPILVDQGTADQFLE
                     SQLHPHLLEQAAKQVGYPLTLRRQTGYDHSYYFISTFIEDHLRHHHRVLNRE"
     gene            complement(884..1978)
                     /locus_tag="VF182_17420"
                     /pseudo
     CDS             complement(884..1978)
                     /locus_tag="VF182_17420"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
     gene            complement(1996..2166)
                     /locus_tag="VF182_17425"
     CDS             complement(1996..2166)
                     /locus_tag="VF182_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438920.1"
                     /translation="MAGIFVLAALLWVTEALPLFATSLLVVGLQMVSWPIPAIGPVWV
                     FNPARRRPIATF"
     gene            complement(2362..3972)
                     /locus_tag="VF182_17430"
     CDS             complement(2362..3972)
                     /locus_tag="VF182_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEX6438921.1"
                     /translation="MKLTVKQCADSGLGFSLLFFCLSAGLVGGCSTLPSLVNRTVSTA
                     IFNTGNTKLGREISPLVESHPGESGIYPLPDARDAFAARVLLAQAAERTLDLQYYIWH
                     GDMSGTLLFNAVRDAADRGVRVRLLLDDHGTSGIDTTLAALDSHPNIEVRLFNPFVIR
                     KPRGINYLFDFFRLNRRMHNKSFTADNQVTIIGGRNVGDEYFGVGEGLLYVDLDVMAL
                     GPVVSQVSTDFDRYWASESSYPVAGLLPAVSPARIAEIAAAASLIKKDPAAIAYIDAI
                     RNSSFVRELVERRLPLQWAATRMISDDPAKGLGLAPPEELFPYKLEKIIGPPEIEVDL
                     VAPYFVPGADGTEEFVALAQRGVRIRILSNTFEATDAVGAVHAFYAKRRKALLKGGVE
                     LYELRRLAPVVEQRGNGSPTGSSGSSGSAGSGDSSSSSLHAKTFSVDRARVFIGSFHF
                     DPRSADLNTELGFVIDSPALARQIRTVFDERIADYAYEVRLSEDGDLYWIEYRDGQRV
                     QHDTEPGTSVWERAGLRFLSLLPIEWLL"
     gene            4156..5472
                     /locus_tag="VF182_17435"
     CDS             4156..5472
                     /locus_tag="VF182_17435"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6438922.1"
                     /translation="MKETPKRVVIVGGGFGGLYAAKTLSNQAIEVTLIDRKNHHTFQP
                     LLYQVALAVLSPAEIASPLRHILHKAPNVRTILGEVGGFDTVARRVKLTGGGELDYDY
                     LIVAAGARHAYFGHDEWAEEAPGLKTIEDAVEIRRRLLLAFEKAERAALLSGKQEPPT
                     FAVIGGGPTGVELAGAIADLARLVLAKDFKAIDTRKARVRLYEGSARVLGTFSEESSR
                     SAERQLQELGVEVFTESMVTAVEPGRIKVGEEWVATDVTLWATGVAASPLGKELAVKT
                     DRAGRVPVEADLSLPGHPEVFLIGDMSALTDVNGTPVPGLAAAAIQQGKVAAQNILRD
                     LRGEKRLPFKYEDRGTMATIGHHRAVAEFGKKRFSGVIAWLLWSLVHIFLLIGFRSRV
                     TVMGQWIWAYLTRAGASPLITEYQRAESHGNRAREETKVPAGMRKT"
     gene            complement(5612..>5880)
                     /locus_tag="VF182_17440"
     CDS             complement(5612..>5880)
                     /locus_tag="VF182_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438923.1"
                     /translation="LEKGSYMRLRRFARCGACLLMLLFAAGCTTYYRVTDQSTRRAYY
                     TTDIDRADSGAVRFYDEKSRASVTLQSSEIVEISKEAYNSGIKE"
BASE COUNT         1336 a         1716 c         1690 g         1138 t
ORIGIN      
        1 gctgatcaaa acccacttcc ctgccgatcc ggaacgcgct ggaatcttcg gccactcaat
       61 gggtggccac ggcgccatca cgattgcact caagaatcca aaactctatc ggtcgatctc
      121 ggcgtttgcg ccgatcgctg cgcccaagca gtgcccgtgg ggtgaaaagg cgttttcaaa
      181 ctatctcggt gcacagcgtg acagttggga cgaatatgac tccacggaga tcttgcttaa
      241 gcttcaggat gctccacggc ggacgccgat cctagtcgat cagggcaccg ccgatcagtt
      301 tctcgagtcc cagctccacc cgcatctgct ggagcaggcc gccaagcagg tgggctatcc
      361 gctcacgctg cggcgccaaa cgggctatga tcacagctat tatttcatct cgacatttat
      421 cgaagatcat ttgcgccatc accaccgggt actgaaccga gagtgaccgc tctagcgacc
      481 gcgggaccgc agcagcctgg tgatgcgtgc taacgggcga accgctcatg tcagctgggc
      541 gccagcgaag catctcagat tatgggtctg agatgcttca ccggacttat tctgagccag
      601 cccgaaggag cttggaacga cgttccccca cacagctcaa agttaataag gcgtaagaga
      661 gcttgtgcgt cctccagttc ggccgttcaa ctcggattcc atgcttgagc ctctgttttc
      721 gcaaacgaga cttgaacctg gagtagcgaa gacacccaac tgcgctgttt cacctcaaac
      781 gggcagcggt tctcatcgtg atgtgcgtcg agatcttaaa tccgccatcc ggcaacggct
      841 tgagttgccc gtctataaat tgttttcaca gctgaattta taatcacttc ggcaaacccc
      901 agaaacgcag gacccatccg ctaccgagaa cgatggccgg cacggtgagc gcgccgatcg
      961 caaacgcgac gcgcgccaga tcgcggctgg cgacctcgcc actcgcataa gccagtgcgt
     1021 tcggcggagt gctgatgggc agcgccatcg acatggaggc tgccagcgcg atgctgatgg
     1081 caacttggat cggataaagg ccgccctgcg cgtcgaagat tgcggcggaa gaaagaccga
     1141 tgggcaggaa cagattggcc gcggccgtgt tcgacatgaa cgtgcccacc accaacgtcg
     1201 ccatgaccag aacgaccaat aaccaaaacc cgctttcgcc cgttgccggc aaccattgcg
     1261 ccaccgcttg atccagcccg gtcaattgca ttcccgttcc cagcgagatg ccaccgctta
     1321 ttagaatcag aatgctccat tccaggcgcg ccaggtcctc atgatcgatt atccccgtgg
     1381 tggtgagcac gacaaccaac agcagcgcca ccacggccgg cgggagccca tgccacaggt
     1441 cggtcatcca caacagcacc gtgatcgcaa aaccgatcac cacgaaccag ctccgcccgg
     1501 tgagtctttc attaaaatgt gccagccgca gcccgtgagt cgttggcgca aagaatctcc
     1561 acaacaccaa ccatgtgaac agcgtcatgc caacggccaa cggcacggcc accaacatcc
     1621 aatcaagaaa cgcgattgta tgcccggatt tttgcaaaaa actgattgcc accgcgttcg
     1681 gcggcgaggc gatcggcgtg ctcatgccgc tgatgttcgc cgtgaaagcc acagcgagca
     1741 ccagcgcttt tcgaaatggt tcctgcgggg gcagcgcgac gagcgcctac atcatcgccg
     1801 ctgtggccgt gttgctcatc cacatgccga acagcaaggt tatcagcatc agacccagga
     1861 gtacccagcg cggttcgccg ccgcagcgcc gcaagagcag tgtggatatc gcgcggtcca
     1921 cgccctcttt taccgccgcc cgcgccaaca cgaaaccgcc aaagaacaac accagcaccg
     1981 ggtcggcggc agtgctcaaa atgtcgcgat aggacggcga cgcgccggat tgaaaaccca
     2041 aaccgggcca atcgccggga ttggccagga gaccatttgc aggccgacga cgagcagcga
     2101 cgtggcaaac agcggcagcg cctcggtgac ccaaagcaac gctgccagca caaagattcc
     2161 ggccataaac cctgcctcgc gactaacgac atccgttctt gttagaatcc atccggcaga
     2221 caccgcgagc gcgactgggc cggcaagaag gagccacgga ggggcgctct tgagtggtgc
     2281 tgttccccgc tcgtgatctg aacgcgggcc gttattcaag gcaacgaatc cgatcggcgg
     2341 caggatttcc gatacgacga atcacaagag ccactcgatc ggcaagagcg acaggaaccg
     2401 gagccctgcg cgctcccaaa cgctcgtgcc cggctccgta tcatgctgca cccgctggcc
     2461 gtcccggtac tcgatccaat aaagatcgcc atcttccgat aaacgcactt cgtaagcata
     2521 atccgcgatc ctctcatcga aaaccgttcg gatctgccgc gccagcgccg ggctgtcgat
     2581 aacgaagccg agctctgtat ttaaatccgc cgaacgcgga tcgaaatgga acgaaccgat
     2641 gaaaacgcgc gcgcgatcca ccgaaaaggt tttcgcgtgc agactcgagc tggagctgtc
     2701 gccggagcct gcgctcccgc tcgaaccgga gctgccggtt gggctgccgt ttcctctctg
     2761 ttcgaccacc ggcgccaaac ggcgtagctc ataaagttcg acaccgccct tgagcagagc
     2821 cttgcgccgc tttgcgtaaa acgcgtgaac ggcgcccacc gcatccgtcg cctcgaaagt
     2881 gttgctcagt atcctgattc taactccgcg ttgagccaac gcgacgaact cttcagtgcc
     2941 gtcggcgccg ggcacgaaat aaggggccac gagatccacc tcaatttccg gtgggccgat
     3001 gattttttcg agcttgtaag gaaagagctc ttcaggcggg gcgagaccga gccccttagc
     3061 gggatcgtcg ctgatcatgc gggtggcagc ccattgcagg ggtaacctcc gctctaccag
     3121 ttcacgaaca aacgaagagt tgcgtatggc gtcgatgtag gcgatggctg ccggatcctt
     3181 tttaatcagc gaggcagccg ccgcaatctc tgcaatccga gcgggactga cggccggtaa
     3241 caatccagcg acgggatatg aggattcgct ggcccagtag cggtcaaaat ccgtggagac
     3301 ctgactgact acgggcccga gcgccatcac gtcgaggtcg acgtagagga gaccttcgcc
     3361 gacgccaaaa tattcatcgc caacgttgcg gccgccgatg atcgtgactt gattgtcggc
     3421 ggtaaacgac ttgttgtgca tgcgccggtt taaccggaag aagtcgaaga ggtaattaat
     3481 cccgcgcggt ttacgaatta caaaaggatt gaacaaccga acttcgatat tcgggtgaga
     3541 atcgagtgcg gcaagagtcg tgtcgattcc ggacgtccca tgatcatcga gcaacagccg
     3601 aactctgacg ccgcgatcgg cggcgtcgcg aactgcgttg aatagcaagg tccccgacat
     3661 gtcgccgtgc cagatgtaat attgaagatc gagagtccgt tccgccgcct gcgctaatag
     3721 cacccgagcg gcaaaggcat cacgcgcatc cggcaatgga taaataccgg actctccagg
     3781 atgagattcg actagcggcg aaatttcgcg cccaagtttg gtattgccgg tattgaaaat
     3841 cgccgtcgaa acggtgcggt taacgagcga gggcaaggtg ctgcatccgc cgactagccc
     3901 tgcgctcagg caaaaaaata gaagggaaaa cccgaggccg ctatccgcgc actgcttcac
     3961 tgttagtttc atcgaggtgt ggataattgg tatgaggctg cgtacttgat cgagcggctt
     4021 atagttatag cctaaaaatg gtttctgctt tctgcgtaat ctaaggctgt tcgcgattga
     4081 atatttcaca ctccacctta aggcggtcaa ctagttgggt caatcaatcc aacggtagat
     4141 acgggaaatg cttcaatgaa agaaacacct aagcgagtag tcatcgtcgg tggcggcttc
     4201 ggcggtctct atgcagcgaa aacactgtcc aaccaggcga tcgaagtcac actgatcgac
     4261 cgcaagaatc atcacacgtt tcagccgctg ctttatcagg tggcgctggc cgtgctctcg
     4321 cccgccgaga tcgcatcgcc tttgcggcac atcctgcaca aagcgcccaa cgtgcgaacg
     4381 attctcggcg aggttggcgg attcgacacc gtcgcgcggc gggtgaagct aaccggcggc
     4441 ggagaactcg attacgatta tctgatcgtc gcggccggag cgcggcacgc ttactttggt
     4501 cacgacgaat gggctgaaga ggcgccgggt ttaaagacga ttgaagacgc cgtggagatt
     4561 cgccgccggc tgctgctcgc attcgagaag gcggagcgcg ccgcgttgtt atcgggaaaa
     4621 caagaaccgc cgaccttcgc ggttattggg ggcggcccga cgggggtaga attggccggg
     4681 gccattgccg atctcgcgcg gctggtgctg gcaaaagatt ttaaagccat cgacacccgg
     4741 aaagctcgtg tgcggcttta cgagggttct gctcgggtat tgggaacgtt ttcggaagag
     4801 tcgtcgcgga gcgccgaacg ccaactgcag gagttgggag tcgaggtgtt caccgaaagc
     4861 atggtcacgg cggtggagcc cggacggata aaagtcggcg aggaatgggt tgccacagac
     4921 gtgacgcttt gggcaaccgg agtcgccgcc tcgccacttg gcaaagagct tgccgtaaaa
     4981 accgaccgcg ccggccgggt gcccgtcgag gcggatttga gccttcccgg acatcccgaa
     5041 gtttttttga tcggcgacat gtcggcgctt accgacgtca acggcacgcc ggtgccgggt
     5101 ctcgcggctg ccgcaattca acagggaaaa gtagcggcgc agaatatcct gcgcgatctg
     5161 cgcggcgaga aacgtctgcc gttcaagtac gaagatcgcg ggacaatggc gaccatcgga
     5221 catcaccggg cggtggcgga attcggtaag aagagatttt cgggtgtgat cgcatggctc
     5281 ctgtggtcgc tggttcacat ttttcttctc atcggtttcc gcagccgcgt tacagttatg
     5341 gggcaatgga tatgggcgta cctcacgcga gctggagcga gtccgctgat taccgagtat
     5401 cagcgggcgg aatctcatgg gaatcgagcg cgagaggaaa ccaaagttcc cgccggcatg
     5461 cgtaagacat gagagaaagc ggcgcaaacg cagcacattt tacgaatgat gctgacccgc
     5521 gagtgctgcg cgcacgaagt ttgcgctttg gctctggggc gagatcgcgt tcttccggca
     5581 catgctaccg gtatttgcga acggttaaat cttactcttt gatgccggag ttataagctt
     5641 ctttagagat ctcgacgatc tcggaggact gtagtgtgac gcttgcgcgg ctcttctcat
     5701 cgtaaaaccg caccgcgccg ctgtcagctc gatcgatatc ggttgtatag taagctcggc
     5761 gcgttgattg atcggtgacc cgataatagg tcgtgcagcc ggctgcaaat aacaacatca
     5821 aaaggcaggc gccacatcga gcgaatctcc ttaatcgcat ataacttcct ttctctaaac
//
LOCUS       DASUVS010000326        12221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_136424, whole genome shotgun sequence.
ACCESSION   DASUVS010000326 DASUVS010000000
VERSION     DASUVS010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12221
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136424"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VF182_17445"
     CDS             complement(<1..875)
                     /locus_tag="VF182_17445"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6438924.1"
                     /translation="MKTLIERLASITSDRWITRDPAVLDNLSWDALSEGRIHPLRSFD
                     ANSPACAVFPASVDEVRRIVFLANEEKIPIIPYGGGSGLMGAALSVTPGIVVDLRRMN
                     QVLDIDVGARCARVQAGIVLETLDQQLTEQNFILGHDPWTLPVATVGGAISTNSVGYR
                     AGVYGSMGEQVLGLETVMANGEILRTRAVPRASTGPDLRLLLIGGEGCFGIITEATLK
                     IFPKPEKRVLRTLFFESFEPGFEAIQKLFAAGLRPALLDYGDDDEKSRHGAVLYLAFE
                     GAVEIVTAEDRLAIDV"
     gene            complement(991..1428)
                     /locus_tag="VF182_17450"
     CDS             complement(991..1428)
                     /locus_tag="VF182_17450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438925.1"
                     /translation="MIVSASPMVKISVVFLVTAALTVFAPLATASGAGGSRGNFASSG
                     PRKDFTDRPSDPRIPGFQRRHPSRIIVVPAPSYYYSPYYFVPGPAFVNASYFCLEHGA
                     GFVSRVGFLDHLGGTHKFALEHAAAICPADVETCVWDGVWRLY"
     gene            1602..2468
                     /locus_tag="VF182_17455"
     CDS             1602..2468
                     /locus_tag="VF182_17455"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6438926.1"
                     /translation="MLAISLALLGSFWFAVSMIFINRGVLAVDYFKGLLANLAVNALC
                     LCIYLSSSSDRIDLWAPANLLFVIIGIFVPGVSRFFIFKGMERLGAPISACLTNSTPL
                     FAILFAVFFLGERPTFTNLLGAFSIVIGIISLSWRGTAKTWRSSDLLFPLTAAFLFAG
                     RDNLVRVGVLQIGSPIMGAAIASVVSLATMAFIYGAFAEHKPLGALGLRGLGLFTVSG
                     FMNFLSYVFIYTALSMERVSLISPLINASSLFVLPLSLIFLKDVEKLTSRKIGATAMV
                     IIGVLLISWEKL"
     gene            complement(2617..3960)
                     /locus_tag="VF182_17460"
     CDS             complement(2617..3960)
                     /locus_tag="VF182_17460"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX6438927.1"
                     /translation="MKRTRWHSSKLIDRLYPPEQRRRSAALAVLLGVLATVYFVLPAP
                     APESLSVEEDAAIRFPRASQSDPRYHALNEDSATESPAKSIDLALRRGDTLHGALLRH
                     GLTPASARELIKELQPFYNPRRMRAGDTFRLLLDHGNGIQGVEHSTNGAVIRVMSTES
                     GWIATRHELPFVRRSKVVRGNIRDSLFEDGVDAGLSPSEIHQLVNLFEYDIDFFSDLR
                     RGDDFSVIFEEKQYANGQREKGKLLAAEIQAGGSPYQIFYYRGKNQKGSYYDKRGEAL
                     AKAFLRAPLNYQRISSPFRINRSHPIFRTIRPHQAIDYAAPAGTPVVAIGDGEVTFSG
                     TRGGYGQMVEIRHDDGYVSRYAHFSKIPKGIRTGKSITRGEVVGYVGQTGHATGPHLH
                     FEMLKRGQKINFLDLKLASADRLTGSELQEFFAMRDQKLALLQSSVAAASGNPHS"
     gene            complement(4062..4772)
                     /locus_tag="VF182_17465"
     CDS             complement(4062..4772)
                     /locus_tag="VF182_17465"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX6438928.1"
                     /translation="MQYQARYATFGGGGAVTPAVKFLLIANISAFVLQALVDYRLVYF
                     FGLVPQLVWQNLYLWQIFTYQFLHGGLFHLLLNMLALWMFGCDLERRWGSSFFLKYYF
                     VSAVGGGILNTLLAPHQMVPSIGASAGVYGILLAYGLIYPNQIIYFYFLFPIKMKHFV
                     WIIGAIALYSSISSGQSGIAHLAHLGGMLFGYLYLRGANPFDRFKLFFAQRRLARMKR
                     RFRVFEGGKDDDSKPTLH"
     gene            5060..6253
                     /locus_tag="VF182_17470"
     CDS             5060..6253
                     /locus_tag="VF182_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX6438929.1"
                     /translation="MSIRGKTAVVGIGETPTDRLGSKPGEPRKSTAEYLAWAARLAMT
                     DAGLSKKDFDGQGLAAIYTTNHSQPFWPEEVAAILGITPGVSLAGGNGGASSVSLLGH
                     AAAAIVAGLCDLVLVIAAAAPFSERGAHRGAPADTRDYEMPFGVMGPNCKISFVMSRY
                     MHESGMTEDHFGKIAVTGRYHASLNPNAYLRKPITIEDYKKSRLISDPIRLFDCVMPA
                     NGGKAYIMTSAERARSMPKPPVYLLGWGECNNSSYGPRYRANPLITGIEQAGKRAFAM
                     AGVTHNDIGCLNLYDDYIPIVMIQIEDLGFCGKNDKAFFEKTDFTFKGDLPIQTSGGM
                     INCGQPSTTGGMLHVIETVRQIRGEGADRQVPHIKFGISTGVGAVNYGKNFGCTAAAI
                     LGREI"
     gene            6435..6866
                     /locus_tag="VF182_17475"
     CDS             6435..6866
                     /locus_tag="VF182_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEX6438930.1"
                     /translation="MTDENRSIKKPIPEITPVNQPFWDSAKVGKLMIQKCRDCGSWVF
                     CPRPVCGDCASDKLDWVEVSGKGKVFSFTVIREVVGQALRGFAPDIPYVTAWIDLDEG
                     PRFCSNIIGCPIDKVHIDMPVQVVFEDSGQGVTLPKFRPAT"
     gene            complement(6926..7786)
                     /locus_tag="VF182_17480"
     CDS             complement(6926..7786)
                     /locus_tag="VF182_17480"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6438931.1"
                     /translation="MSSELWAIAAAAAWAADSVLVRKGTAFSNPSTAALISFSINAAV
                     LLPYIIYQYPSEKIFQSANLFFVLSGIIQPAIVRVMFYVGIVRLGVSRAAPIRGTSPF
                     FSVAIAFFLFRERPELLVYLGGLLTIAGTWLVSYKRAGEAKWRAIDLLFPLGAAMLAS
                     VSQNIRKAGLNTTNEPVIAATLTTATSLVCLLGSLLFSGKRRAIKIDRQSLPFYGAAA
                     VFALIAQLCTFIALNGAQISIVAPLINTTPLFAIAFTALFLRGEEKITPLVLIGVVLL
                     VAGIGFITAR"
     gene            8016..8573
                     /locus_tag="VF182_17485"
     CDS             8016..8573
                     /locus_tag="VF182_17485"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6438932.1"
                     /translation="MKKLLSLLAISGAVLGALFFLTSLPGNGAGGSPAPTADLQPATS
                     VQQGVLVSIEYTLTDEAGKVIESNAGKEPLSYVQGAGQIVRGLEKELVGMRVGEQKKI
                     QVKPEEGYGRRDPNAVQEIPKDKIPSEAQQVGATLMTQAADGRSLPMRIQEIREKTVV
                     VDFNHPLAGRTLNFDVKVTDLKMSR"
     gene            8603..9871
                     /locus_tag="VF182_17490"
     CDS             8603..9871
                     /locus_tag="VF182_17490"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6438933.1"
                     /translation="MPARFFYGYVILALCFMNMVVMRGVNGSFGVYYISLLDEFGWSH
                     SDGASIASVNFLVYALAAPLVGLAFDRLGPRVLMPLGGALVGIGLLLSSIGGTLPHFY
                     FSYGLIAALGQGALGFVGHNALISFWFVRRRATAIGIASMGQGVGALIMVPLTQFLIS
                     QIGWRSTFMVTASVILLAVVPANALLQRRSPQDIGQLPDGDTAVSAETHGVHGKARPG
                     ARDWRLGEAAVSFPFWCITLGHLALGTALFMINTHIVAHYVVVGYDKLMAAFLAGLIG
                     FVRIGGTVLWGLVSDKLGRDRTYGIATSVAIIGVAGLIAMPLGAPLWYAYLTAILYSI
                     GHSAGNPTYGSVIADIFSGHKVGLIFGFLEISFGLGSALGAWIGGYLFDVTGSYASPF
                     SLCLICFAISGLSIHSCIQWQRRQLRSTLT"
     gene            9942..10949
                     /locus_tag="VF182_17495"
     CDS             9942..10949
                     /locus_tag="VF182_17495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6438934.1"
                     /translation="MAITFCLHGSARTIKEAVARAKKAEDLGFEAIFFADSHMNNVDC
                     YQVMAMCAVATKKIRLGTAVTNMVYRDPTITANSFATLNEISEGRAIIGMGTGDGPVY
                     SLGRTATRLVDFEKGLQTMRDLLHDRGIEVPRTRERAAGGRVNLKAGRRPVPIYISAE
                     GPKTLRLAGRLCDGVILGTGFDPEVLEWARARIADGAGDAGRPLADIDIMPAGMIVVD
                     DDGDRARQRVRSRMANRAHHNFRFTMETVPELERAGVERFMNGFDISKPIEERVDPDL
                     ITQYLLQRFTIAGNPEECVARIKLLEAAGIKRLLLTPPNAIYDRVMEAWGDRVIPCCG
                     N"
     gene            11158..11826
                     /locus_tag="VF182_17500"
     CDS             11158..11826
                     /locus_tag="VF182_17500"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438935.1"
                     /translation="MAWTWTWTAVDRWRLAHHSNLVERRPAPNSLGRFNSCPRRHCRR
                     RPGDHRYRHELVNWKIRARKEIRDVKDLVGKKIAIASRELGLQLALKKGSGFNRVKLL
                     SLGSSPTFMSALREGVVDATLFSYPELLIATKEGFTVFANLRQFADLSDTSVVVSRAT
                     LKTAAAAEALLPEVTSKPIARVKTTASRLPAPWPDTPGSKTMLLWRRPDSFSIEGAQH
                     ELRN"
BASE COUNT         2858 a         3354 c         3258 g         2751 t
ORIGIN      
        1 acatcgatcg ctaatctgtc ttccgctgta acgatctcga cagctccctc gaaagctaga
       61 taaagcacgg ctccgtgcct acttttctcg tcatcatcgc cgtaatccag cagcgccggc
      121 cgcaaacccg cggcgaacag cttttggatc gcctcaaaac ccggctcgaa cgattcaaaa
      181 aacaacgtcc gaagaacacg cttctcaggc ttcggaaaaa tcttcaaggt cgcctcagta
      241 atgatgccga aacaaccctc cccgcctatc agaagaagac gcaagtccgg tccggtcgaa
      301 gccctcggca ccgctcgcgt gcgcaaaatt tccccatttg ccataaccgt ttccagccct
      361 agaacctgct cgcccatcga accataaacc ccggctctat aaccgacgct gttggtcgag
      421 atcgcgccac ctacagtggc aaccggtaac gtccacggat catgaccgag aatgaaattc
      481 tgctctgtca attgctgatc taacgtctca agcacaattc ccgcttgaac gcgcgcacaa
      541 cgcgctccca cgtcgatatc cagaacctga ttcatccttc gtaaatcgac gacgattcca
      601 ggggtgaccg atagagccgc tcccataagt cctgagccgc cgccgtacgg aatgatcggt
      661 attttctctt cattggccaa gaagacgatg cgtctgactt catccaccga agctgggaaa
      721 acggcgcacg cgggcgagtt tgcatcaaac gacctgagcg gatggatgcg cccttcgctc
      781 aaggcatccc acgataaatt atccaaaact gccggatccc gggtaatcca tcgatcagag
      841 gtgatcgagg cgagccgttc tatgagggtc ttcatcactt acctgaagct cgctaaatat
      901 agcccgatcg ataatccgcg gaaagcgcgc agaatatgcg cgggctgaat cggacgaaat
      961 ggcagcagct cgctggaaaa tgtttatcag ctaataaaga cgccacacgc cgtcccatac
     1021 gcatgtctct acatcggccg ggcaaatcgc cgccgcatgc tcaagagcga atttgtgagt
     1081 tccacccaga tgatcgagaa acccaacgcg gctgacaaac cccgcaccgt gctcgagaca
     1141 gaagtaggat gcattcacaa aggctggacc gggtacaaaa tagtagggcg aataataata
     1201 gctgggagca ggtacgacga taatgcgcga tggatgacga cgctgaaatc ccgggatacg
     1261 gggatcggaa ggtctgtcag taaagtcttt tctaggaccg gaactcgcaa agttaccgcg
     1321 cgatccgccc gcgcccgaag ccgtcgccag tggcgcaaag accgttaatg cagcagtaac
     1381 caggaaaacc acgctgattt taaccatcgg agaggcagag acaatcataa aatcattgta
     1441 gcagactctg gaccgattgc atctcgctcc gatgagcttg atcgatgacc tatctctaca
     1501 aacgagccgc gcgctgtgtg cccgagctac gcaccaaacg agcttgtgca aggaaccatt
     1561 gaagatcatt tcctcggcag gtaaggtcgc cgtttaattt catgttagca atttccttag
     1621 cactgttagg ctcattctgg tttgccgtct ccatgatctt catcaatcgc ggcgtcctgg
     1681 cagtcgacta tttcaaagga ctgctcgcca acctcgccgt aaacgcgcta tgtctgtgca
     1741 tctatctctc atcgtcctcc gatcgaatcg atctctgggc tccggccaat ctcctatttg
     1801 tgatcatcgg aatttttgtt ccgggggtat cccgtttttt tatcttcaag ggaatggagc
     1861 ggctaggcgc gcccatttcc gcatgcctga caaacagcac gcctttgttc gcgatcctct
     1921 tcgctgtctt ctttcttggc gagcgcccga catttaccaa cctgctgggc gccttttcta
     1981 ttgtcatagg catcatttcg ctctcctggc gcggcaccgc taagacatgg cgcagcagcg
     2041 accttctctt tccattaacg gcggcttttc tttttgccgg acgggacaac ctggtccgcg
     2101 ttggcgttct gcaaattggt tcacccatca tgggcgcggc gattgcgtcg gtggtatcgc
     2161 tggcgaccat ggcgtttatc tacggcgctt tcgccgagca caaaccgctg ggcgcgcttg
     2221 ggctgcgcgg tctcggtctc tttactgttt cgggcttcat gaattttctt tcctacgttt
     2281 ttatctacac cgctctcagc atggaacgag tatcgctcat ctcgccactg ataaacgcct
     2341 cgtcgttgtt tgttctgcct ctgtcgttga tctttctcaa agacgtcgag aagctgacct
     2401 cgcgcaagat cggtgcgacc gcgatggtca taattggagt gttgctgatt tcctgggaga
     2461 agctctagag gagccggcac ggcttgttaa cagtgcgttg cgccgcggca aaccgatctc
     2521 gccgcgactt caatccgagt gctcgatgcc gagccggcat agctgcagga ggtttagagt
     2581 ttatagagga gagcaacgtc tatatattgc tgctcattaa gaatgggggt tcccagacgc
     2641 ggcggctacg ctgctttgaa gcaaagctaa tttctgatcg cgcatcgcga aaaattcctg
     2701 caactccgat ccggtgagcc ggtccgcgga cgcgagttta agatcaagga aatttatctt
     2761 ttggcctctt tttagcattt cgaaatgcag atgcggaccg gtagcatgcc cggtctgacc
     2821 gacgtacccg accacttcac cgcgagtgat cgacttgccg gttcgaattc ctttaggaat
     2881 tttcgaaaaa tgagcatagc gggaaacata cccgtcgtca tgccggattt ccaccatctg
     2941 gccataaccg ccgcgggtcc ccgaaaacgt cacttcccca tctccaatcg caaccaccgg
     3001 tgtgccagca ggggcggcat aatcgattgc ttgatgcgga cgtatggtgc ggaagatcgg
     3061 atggcttcta ttgatccgga atggcgagga gattcgctga taattcagcg gcgctcgcag
     3121 gaatgctttc gccaaagcct cgccgcgctt atcgtaatac gagcctttct ggtttttgcc
     3181 tcgataataa aaaatctggt acggtgatcc gccagcctga atttctgcgg cgaggagctt
     3241 acccttctcg cgttgaccat tggcatactg cttttcttcg aagatcactg aaaagtcgtc
     3301 gccccgccgg agatccgaaa aaaaatcgat atcatactca aacaagttga ctaactgatg
     3361 gatttccgat ggcgaaagtc ccgcgtcaac tccgtcttca aacaaactgt cgcggatgtt
     3421 gccgcgaacc actttgcttc gacgtacaaa tggcagctcg tgtcgcgtgg cgatccagcc
     3481 actctcagtc gacatgacgc gaatcacggc accattggtg gaatgctcca caccctgaat
     3541 tccattcccg tggtcgagca gcaaacgaaa agtgtcgccc gcgcgcattc ttctgggatt
     3601 atagaaaggt tgcaactcct ttatcagctc acgcgctgaa gcaggcgtaa gcccgtggcg
     3661 cagcaacgct ccgtgcagag tatcgccgcg tcggagcgcc aagtcgatgc ttttggctgg
     3721 tgactcggtt gcgctgtctt cgtttaaggc atgatagcgt ggatccgatt ggctggctcg
     3781 cggaaatctg atcgccgcat cctcttcgac gctcaacgat tcgggcgccg gtgcgggcag
     3841 cacaaagtat acagttgcga ggacgccaag aagcacagcc agcgccgccg atcggcgtct
     3901 ctgctccggg ggataaagcc gatcgatcag cttagaagaa tgccaccttg ttcttttcat
     3961 gtcatttgac tctccgcaaa tgatttcccc tttttacaac atctggattc gattacaaaa
     4021 ccggaaggca aaattttttt ctaaggcgaa cgcggttaga actaatgcaa agttggcttt
     4081 gaatcgtcat ccttgccgcc ttcgaagacg cgaaagcgtc gtttcattcg agccaatcgt
     4141 cgctgcgcga agaaaagctt gaaacgatcg aagggattag caccacgaag atacaaataa
     4201 ccgaacagca tgccgcctaa atgcgcgaga tgcgcaatgc cgctctgccc agagctgatt
     4261 gacgaataaa gcgcaatggc gccgataatc cagacaaaat gtttcatctt gatcggaaaa
     4321 agaaaataga aatagatgat ctgattcgga tagatcagac cgtaggcgag caggatgcca
     4381 tatacgcctg cagaagctcc aatgctagga accatttgat gcggagctaa aagcgtattg
     4441 agaatgccgc cgcccacggc ggagacaaaa taatatttta gaaaaaaact gctaccccaa
     4501 cgacgttcca aatcgcagcc aaacatccac agcgccagca tgttgagcag aagatgaaac
     4561 aggccaccgt gtaaaaattg ataggtgaag atttgccaga ggtagagatt ttgccagacg
     4621 agctgcggca ccaacccgaa gaaatacacc agccgataat cgaccaacgc ttgaagaaca
     4681 aaagccgaaa tgttggcgat cagcaagaat ttgaccgccg gagtaaccgc cccaccgccg
     4741 ccaaacgtcg cgtatctggc ttggtattgc acgtctgcgt agcttaactt ctgatcggcg
     4801 gaattggaag gcagcggtct caaaaagaaa aaagtcccgc gattcagaca aaaataaggt
     4861 ccattatcga gccggccggc gacgcttgtt gtcagacgcg cgcatagcga gcgatcgtca
     4921 acaatgtgcg tgacagatag aaagagttac ttgctccccg gccgatcaag aatttctttt
     4981 cgacaacatt tgatccgcac cggcttgaca gctcttcgac gctgcactat ctctcgaaag
     5041 ctaagagagg aattcggaca tgagcattag agggaaaaca gcagttgtgg gaatcggcga
     5101 gacgccaacg gatcgactcg ggagcaaacc cggcgaacct cggaaatcca cagccgagta
     5161 tctcgcatgg gccgcgcgcc tggcaatgac cgacgcaggg ctcagcaaga aagattttga
     5221 tggtcaaggt ttagcggcta tttataccac caaccattca caaccttttt ggccggagga
     5281 agtagccgcg atcctgggta ttacaccggg ggtttctctg gcgggcggaa acggcggtgc
     5341 cagctcggtt tccctgttag gccatgcggc ggctgccatc gtcgccggac tttgcgatct
     5401 cgtgttggtg atcgctgcgg ccgcgccatt ctcggagcgc ggcgctcatc gcggcgcacc
     5461 tgccgatacg cgcgactatg aaatgccttt cggagtcatg ggacccaact gcaaaatttc
     5521 cttcgtcatg agccgatata tgcatgaatc gggcatgacc gaagatcact tcggcaaaat
     5581 tgctgtcacc gggcgttatc acgcgtcgct taatcccaat gcctacttgc gcaagccgat
     5641 cactatcgaa gattacaaga aatcgcgtct gatctccgat ccgatccggt tgttcgattg
     5701 cgttatgccg gccaacggcg gcaaagccta catcatgact tctgccgaac gcgccaggag
     5761 catgcctaag cctccggtct atctactcgg ctggggcgaa tgcaataact ccagctatgg
     5821 accacgctat cgggcaaacc cgctcatcac cggtatcgaa caagcaggca agcgcgcctt
     5881 cgcgatggcc ggcgtgacac acaatgatat cggctgtttg aatctctacg acgactacat
     5941 tccgatcgtc atgattcaaa ttgaagatct tggattttgc ggcaagaacg ataaagcttt
     6001 tttcgagaaa accgatttca ctttcaaagg cgacctgccc attcaaacca gcggcggcat
     6061 gatcaactgt ggccaaccct caaccaccgg tggcatgttg cacgtcattg aaaccgtccg
     6121 tcagatacgc ggcgaaggcg ctgatcgcca ggttccccat atcaaattcg ggatcagcac
     6181 cggagtgggc gcggttaatt acggcaaaaa cttcggctgc acagcggcgg ccattttggg
     6241 aagggaaatc taaaaggcaa gacacgaagt atgaattccg aaattcggaa caatctcaaa
     6301 tgagcaaaat aagaaacttg cgaactgacc cgtttcaaat tcgatttgca attttcctat
     6361 ttgtttcgga gttcgatttt tgaatttcga attttactat tgatcgctta cgattcgaaa
     6421 ggtgagagtt tgatatgacg gatgagaaca gatcgatcaa aaagccgatt cctgagatta
     6481 cccccgttaa tcagccgttc tgggacagcg ccaaagtcgg caaattgatg attcaaaaat
     6541 gccgcgactg cggcagctgg gtgttttgcc cgcgaccggt ttgcggcgat tgcgccagcg
     6601 acaaactcga ctgggttgaa gttagcggca aaggaaaagt attttctttc acggttatac
     6661 gcgaagttgt cggtcaggcg ctgcgcggtt ttgctcccga cattccctac gttaccgcat
     6721 ggatcgatct cgatgaaggc ccgcgcttct gcagcaatat tatcggctgt ccgattgaca
     6781 aagttcacat cgatatgccg gtgcaagttg tgttcgaaga cagcggccaa ggtgtaacgc
     6841 tcccgaagtt caggccagca acttaagaca gatcgttggt tgggttgacc ggagcatcgt
     6901 cgtgacctta cctgaaccgg aaccgctatc gcgccgtaat gaaaccgatt ccggcaacta
     6961 acaggacgac accgatcagc accagcggag tgattttttc ttcgccgcgc aagaacagcg
     7021 cggtgaaagc aatggcaaat agcggcgtgg tgttgatgag cggcgctacg atcgagattt
     7081 gagcgccgtt caatgcgata aatgtgcata actgggcgat tagcgcgaag actgccgcgg
     7141 cgccgtaaaa cggcaggctc tggcgatcga tctttatcgc tcgccttttg ccggagaaca
     7201 gcagggagcc cagcaggcat accagcgatg tcgctgtcgt aagggtcgcg gcaatgaccg
     7261 gctcattggt agtgtttagc ccggctttgc gaatattttg cgacaccgac gctagcatcg
     7321 ctgcgcccaa aggaaacaaa agatcaatag cgcgccactt ggcctccccc gctcttttgt
     7381 atgacaccaa ccacgtgccg gcgatagtca ggagcccgcc caggtaaacg agcagctccg
     7441 gtcgttctcg aaatagaaag aacgcaatcg ccacggaaaa aaacggcgaa gttccccgga
     7501 tcggtgcagc gcgcgaaacg ccgagccgga cgatcccgac gtaaaacatc acccgcacga
     7561 ttgccggctg gataatcccg ctcagaacaa aaaaaaggtt agcggactgg aaaattttct
     7621 cggacggata ctgatagata atgtacggga gcaggacggc tgcattgatg gaaaaactga
     7681 ttaacgcggc cgtcgatgga ttggaaaaag cggtgccttt gcgcacaaga accgaatccg
     7741 ccgcccatgc cgcggctgcg gcgatcgccc agagctcgct gctcacgcta aatggaagct
     7801 ttcatgggat tggcgcagat ttaccacgat tgtaattgtt agagtagaat ctgcttgagt
     7861 tgttgttgag gaaaactgtt taaaccgaga gcctctgcca agcgatggtc ctggaagctg
     7921 tggaaaaatg atcaagcgag tggacaaccg tttcggttgt attcaaccga ggcgcgttat
     7981 atctttccga gattgtcatt tcggccgaat aacccatgaa aaaacttcta agcctgctgg
     8041 caatcagcgg tgcggttttg ggtgcgctgt tttttcttac cagccttccc ggaaacggag
     8101 ctggcggttc accggctcca accgccgatt tgcaaccggc aacttccgtt cagcaggggg
     8161 ttctcgtgtc cattgagtac acccttacgg atgaggccgg taaggtaatc gaaagcaatg
     8221 ccggcaaaga gcctttgagc tacgttcagg gcgcgggaca aatcgttcgc gggctggaaa
     8281 aggaactcgt tggcatgaga gtcggcgagc aaaagaaaat ccaagttaaa cccgaggaag
     8341 gttatggtcg ccgcgatccc aacgccgtgc aagagattcc taaagacaag attccctcgg
     8401 aagcgcagca agtcggcgcc acattgatga cccaagccgc ggacgggcgc tccttgccga
     8461 tgcgcattca agaaatcagg gaaaagactg tcgttgtcga cttcaatcac ccgctggccg
     8521 gaagaaccct caatttcgac gtaaaagtca ccgacctcaa aatgtcccga tagctgggcg
     8581 ttttccactg ccccgatatt gaatgcctgc aagatttttc tacggttacg tcattctcgc
     8641 cctgtgtttc atgaatatgg tggtgatgcg cggcgtgaat gggtctttcg gtgtctacta
     8701 catctcacta ctcgacgaat tcggctggtc gcatagcgac ggcgcgagta tcgcctccgt
     8761 caatttcttg gtgtacgcgc tggcagcgcc tttggttggt ttggcctttg atcgtttagg
     8821 accccgtgtt ttaatgccgc tgggcggcgc cctggtcggc atcggtcttc tgctctccag
     8881 cattggcggc acgttaccgc acttctactt ttcctatggt ttgatcgccg cgcttggcca
     8941 gggagcgctc ggcttcgttg gccacaatgc gctgatctcc ttttggtttg tgcgccggcg
     9001 cgccaccgcc atcggcatcg ccagcatggg acaaggcgtg ggcgcgctta tcatggtccc
     9061 gttaacccaa ttcttgattt cacaaattgg ctggcgttcc acgttcatgg tcactgcaag
     9121 cgtaattttg ctcgcggtgg tgccggccaa cgccttgctg caacgtcgaa gcccgcagga
     9181 tatcggccag ctgcccgacg gagacacggc tgtctctgca gaaactcacg gcgtgcatgg
     9241 taaagctcga cccggagccc gcgactggcg cctcggcgaa gccgccgttt cttttccctt
     9301 ctggtgcatc actctcggac acctggctct gggcacggcg ttatttatga tcaatactca
     9361 catcgtcgcg cactatgtcg tggtcggcta tgacaagctc atggcggctt tcctggcggg
     9421 gttgatcggc tttgttcgca tcggcggtac ggttctctgg ggtttggtct ccgacaagtt
     9481 gggacgagac cggacctacg gtatagcgac gagcgtagcc atcatcggcg tcgccggtct
     9541 gatcgccatg ccgctgggcg cgccgctttg gtatgcttat ctcacggcga ttctctacag
     9601 catcggccac agcgccggca atccaaccta cggctcggtg atagccgaca ttttctccgg
     9661 gcacaaagtg gggctgatct tcggcttttt agaaatcagc tttggcttgg gcagcgcatt
     9721 gggcgcgtgg atcggcggtt acttattcga cgtaaccgga tcgtatgcat cgcctttctc
     9781 cttatgcctg atctgtttcg cgatctccgg gctgtcgatc cactcgtgta tccagtggca
     9841 aagacggcag ctacgcagta cgttaacgtg atttcatttc gccaatgcgc tcagatgttc
     9901 ggcgatggca tagccaaatt actcgatgaa aggagatctt catggcgata actttttgct
     9961 tgcacggctc ggcgcgcacc atcaaagaag ctgtggcacg cgctaagaaa gccgaagacc
    10021 tcggtttcga agcgattttt ttcgccgaca gccatatgaa caatgtcgat tgctaccagg
    10081 tgatggcgat gtgcgccgtg gcgacgaaaa aaatccggct gggcacggcg gtcaccaata
    10141 tggtctatcg cgatccgacg attacggcca atagctttgc cacgttgaac gagatttccg
    10201 aggggcgggc gattatcggc atgggcactg gcgatggccc ggtttacagc ctgggtcgaa
    10261 ccgccacccg gcttgtcgat ttcgaaaaag ggcttcagac catgcgcgat ttgttgcacg
    10321 accgcggcat agaagttccc aggacccgcg agcgcgccgc gggaggtcga gtcaatctga
    10381 aagccggcag gcgtccggtg cctatttaca tttcagccga gggtccgaag accctcaggc
    10441 tggcagggcg gctttgcgat ggagtcattc ttggcaccgg gttcgacccg gaagtactcg
    10501 agtgggcccg agcgcgcatt gccgacgggg ccggggatgc cggccgacct ttggccgaca
    10561 tcgacatcat gccggcgggg atgattgtgg ttgacgatga tggcgatcgg gcgcgccagc
    10621 gcgtgcgctc gcgcatggcc aatcgcgcgc accataattt tcgtttcacg atggaaacgg
    10681 taccggagct cgaacgtgcc ggagtcgaga gattcatgaa tggatttgat atctccaaac
    10741 ccatcgaaga gcgggtcgat cctgatttga tcacccaata cttgctccag cgcttcacga
    10801 tcgccggcaa ccccgaagag tgcgtcgcgc gcatcaagct actggaagcc gccgggatca
    10861 aacgattgct gctcaccccg cctaacgcca tttacgatcg ggttatggaa gcctggggcg
    10921 atcgggtgat tccgtgctgc gggaactgat cccattctgt aacttcaatg gtttgcaaga
    10981 tttatgaaaa ctcaattgca tcggctcatt tcgtagcgtt aacttatacc gctctgggcc
    11041 tgattgacat cagtggggct aacgcgcaga ggacagaaat acgcgcaagc ttcgccggcg
    11101 cgaccgggta tcacttgccc atctggttgc agaagcagga ggctctcgac cgaaagtatg
    11161 gcttggacat ggacctggac tgctgttgat cgctggcggc tcgcgcatca ttccaacctt
    11221 gttgagcggc gacctgctcc taactcactc gggcgtttca acagttgccc gcgccggcat
    11281 tgccggcgcc gacctggcga tcatcgctac cgtcatgaat tagtcaactg gaagatccgc
    11341 gcgcgcaaag aaatccgcga tgtcaaggat ctcgtcggca agaaaattgc cattgcctcc
    11401 cgcgagctcg gcctgcagtt ggcattaaaa aaggggtctg gattcaatag agtcaaactg
    11461 ttgagcctcg gttcgagccc gacgttcatg agcgcgctcc gcgaaggcgt cgtcgatgct
    11521 accctgtttt cttatcccga gcttttgatt gcaactaaag aagggtttac cgtgttcgcc
    11581 aatctgcgtc aattcgcgga tctttccgat acttcggtgg ttgtcagccg cgccaccctg
    11641 aaaacagcgg ccgctgctga agcgcttctt ccagaggtta cgtcgaagcc tattgcacgt
    11701 gtgaaaacaa ctgcgagccg cctaccggcg ccttggcccg atacaccggg atcaaagacc
    11761 atgctgcttt ggaggagacc cgacagtttt tcgatcgagg gagctcagca cgaattgcgc
    11821 aactgaaagc cgatcagcga agcatggcgc ttgtccaact ttaccaaagc ctaaaccaag
    11881 aacttacgcg gcttgatgga tatggcaacg cagggctctg ccagggtatt cactatgacg
    11941 acatcttcac cgtagacgca agcggcgaac caacagtttc caaagtagac cccttgccaa
    12001 ccgagatttg cagattatat ttacgtcgtc tcggctcaga gacactaaaa acacgcgttt
    12061 tttcagaggt gaagcatggc actggtaaga cttgagaaga cgctcgcccc cgcattttcc
    12121 ccatcaggca gacctcttcg tatcgcgcag atagcaccct tgtatgagag cgtgccgcca
    12181 aaactttatg gcggcaccga acgcattgtc gcctacttgg t
//
LOCUS       DASUVS010000327        16507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1010356, whole genome shotgun sequence.
ACCESSION   DASUVS010000327 DASUVS010000000
VERSION     DASUVS010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 16507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16507
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010356"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(560..2272)
                     /locus_tag="VF182_17505"
     CDS             complement(560..2272)
                     /locus_tag="VF182_17505"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6438936.1"
                     /translation="MHGGQAMLKAIERSGIDYIFSSPGSEWPPIWEALAEAAAGGAQK
                     PKYINCRHEAVAVAMAAGYTKVTNRPQVVMLHATAGPLNAAMTLRAALHERTPMVILS
                     GEIAAYGENTQIADPGGQWLHDLTDLGGSPDLLRGCVKWADRISSAELLAPTIERAVQ
                     IAVEPPAGPVLIGIPFECMMEEVQWIDHGKTNEVVRSVALEDKAIDQALKLIAGAKNP
                     IAVTEYVGGDPRAVESLVELCDLFSIPVMETYRPAFMNFPRTHPLYLHHDSKAIDAAD
                     LILMVEAVTPWYPASKAPKSAKVIAIAAEFPNSRLPYWGYNVDLALVAPPAQTLEKMV
                     AKAKASEEVAGNRAAYEQRLGRTRQQHGDYFGKLKTEARKHADDTPIDPRWLCHVLAE
                     ALPDNSVISEETTVYRTLIQEAIPRDQPQSYFARITGGLGVGLSYALGVKLAMPDRPV
                     FALIGDGAFHYNAVPSCLGVAEEYHLPIHVVVFNNGRYLSMETSLIKYFPEGAAKKTG
                     VHFGAHIAPHPDYQYYAQAHGGRGFRVTRPDEIKPAVEQALLQEREGKLTVIDVVLSD
                     HNPR"
     gene            complement(2391..3407)
                     /locus_tag="VF182_17510"
     CDS             complement(2391..3407)
                     /locus_tag="VF182_17510"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438937.1"
                     /translation="MAIRLRVFFAPILFIGIFLTHEASPASGELLVAYGGHNETMAPM
                     WVGVDRGLFRKHGVDPRVLQTRSGPIMMATLASGGASLVWAAPTSAISAATGGMKLGC
                     FAVGNDTIPRELIARKGIESLEDLRGKTFGVQSIGGGFWLATMAVLNGLDIDPDKYKL
                     NMRVIGDTGTVTQALSAGNIDAAVVPYSYADIAKRAGARSLADAATVKLIYQGIAMCA
                     RKDSAAISNETMIALTKGIVEALVYIQEPANKRDVSEILKKHLRLTRDEDIEASYKVT
                     RLQMPTLDIAPNGEAWKRIQRLLIRINPKVQDADLDQLLMSNAARTLEESGFLSEMRK
                     RLGR"
     gene            complement(3578..6202)
                     /locus_tag="VF182_17515"
     CDS             complement(3578..6202)
                     /locus_tag="VF182_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX6438938.1"
                     /translation="MAIDPICGMTIDPARSAGKHEHNGQTYYFCSKHCLAKFNNDPAR
                     FASSVVQGSAQNKSVADPVCGMTVEPGTAAAKHEYNGMVYYFCSHHCAQKFKEDPEKY
                     SNAKHAGHAGHDNDHRPGGAQTPPKAKPAEPVYVCPMDPEVRASKPGACSKCGMALEP
                     ETLAAPATRTEYVCPMHPEIVRSEPGSCPICGMALEPKTVTLEDAPNPELVDMIRRFW
                     ISAALSLPLFIVAMSDMISGQPLQHVLSPRLLTWLQFVLATPVVLWGGWPFIQRGWYS
                     IVNRNLNMFTLIAIGVGAAYLYSVAATLFPDIFPESFYGHSGTVAVYFEAAAVITTLV
                     LLGQVLELHARSQTSSAIKALLGLSPKTARLLYQDGREEDVSLDLIKAGDRLRVRPGE
                     RVPVDGVVIEGATSIDESMITGEPIPVEKSKGSKVTGGTVNGTGSVVMEAQRVGSETL
                     LAQIVRMVSEAQRTRAPIQRLVDIVSAYFVTIVLIVAGVTFAVWAFFGPEPRMAYAIV
                     NAVSVLIIACPCALGLATPMSIMVGVGRGAAEGILIKNAEALEILEKVNTLVVDKTGT
                     LTEGKPQLSEIITMNSGSDRKELLRLAASIERASEHPLAAAIVAAAEKEGARLGNTEE
                     FRSLTGRGVIGKVEGRSIALGNIKLLEELSTDPGPLRARAEELRSGGHTVMFVAIDDR
                     AAGLVAVSDPIKQSTPEAIKILHGENVRIVMLTGDSRTTAEAVARRLEIDRIEAEVLP
                     EQKAEVIKRLQAEGRVVGMAGDGVNDAPALAQAHVGIAMGTGTDVAIESAHITLVKGD
                     LRGIAKARRLSRATMRNIRQNLFFAFFYNVLGVPIAAGVLYPFFGILLSPIIASAAMT
                     LSSVSVIANALRLRRIAL"
     gene            complement(6376..9576)
                     /locus_tag="VF182_17520"
     CDS             complement(6376..9576)
                     /locus_tag="VF182_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEX6438939.1"
                     /translation="MIERIIEASARNKFLIFLMVIFLSAMGIWALKNTPLDAIPDLSD
                     VQVIVYTPWMGRNPTIMEDQVTYPIVTTMISAPKVKFVRGFSDFGFSYVYIIFEDGTD
                     IYWARSRVLEYLNQVSGRLPKGVAPTLGPDASGVGWVFQYALVDETGKYDLAQLRSLQ
                     DWYLRYQIASVEGVAEVASLGGFVRQYQVNIDPNRLAGYGLSPKTVVEAIRMSNNEVG
                     GRSIELSGAEYMIRGRGYIRSTQDIEAIAVGGERGTPILVRDIASVTLGPDMRRGIAE
                     LNGRGEIVSGVVVMRYGENALRLIERVKEKIKEIEPSLPPGVKIVTAYDRSDLILRSI
                     ATLKEKLIEESLIVSVVIIVFLFHLPSALVVIITLPIAIIISFIAMHSLNLTANIMSL
                     GGIAIAIGAMVDASIIMVENAHKKLELWEEQGRPGNRTDVLIEAAKEVGKPLFFSLLI
                     ITVSFLPIFTLEGQEGRLFKPLAYTKTFAMFFAAFLSITLAPVLLLLLVRGRVRPEAR
                     NPVNRFLIALYMPVIHWVLRFRKTTIALAVLALLLTIPAFMRLGSEFMPPMNEGMIFY
                     MPTTLPGISITQSARLLQMQDRILKSFPEVQWVLGKAGRAESSTDPAPFSMGETLVML
                     KPEQEWRQLPVDRSDWPGPLRPIGEVLFGTTRTITRDELVADMDKKLRLPGVANAWTM
                     PIKNRIDMLSTGIRTPIGIKIFGPDLGKIEEIGKNLEAALQMIPGTRTVFAERVTAGY
                     YLDFELRRTEIARYGLAIDEVQMLIESVIGGESITTTVEGRERYPVSVRYARELRDDP
                     ERLKRILIPGMNGAQVPIGHLADLRLTSGPAMIRDEDGQLSGYVYVDMTGRDIGGYVE
                     EAKKKVAEQVTLPTGYTLSWSGQYEYMQRAQQKLIYVVPLTLLVIFLLLYINFKSVGR
                     CAIVLLAVPFSMIGAIWLLYLLEYNMSVAVWVGIIALAGVDAETGVIMLLYLEHAYDK
                     WRSEGRMRNINDLHEAIIEGAVKRIRPKVMTVMAILMGLLPIMWSHGAGADVMKRIAA
                     PMIGGVISSFLLELLVYPAIFDIWRGWKMRRAIQKA"
     gene            complement(9675..11219)
                     /locus_tag="VF182_17525"
     CDS             complement(9675..11219)
                     /locus_tag="VF182_17525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX6438940.1"
                     /translation="MKIIKILGALVLLAGVFAIGYWRGQSDPKADHALSGHSATEAST
                     DSQSMQGHDMSSGGVNVSSEMQQLVGIRTATAEIRPLMQKIRTVGIVTYDETRIAQVF
                     SKVEGWIEQLLVNYTGTLVRKGQPLFTLYSPDLVATQEEYLLALKAKQTLGSSSIKEI
                     SAGSDSLLESAHRRLSLWDISEEQINNLEKTGKPQRTLTFFAPISGFVIKKDAVQGMR
                     VMPDKELYTITDLSTVWVNADIYEYELAHIRVGQKAKMNVASYPARDFVGKVSWISPV
                     LEEKTRTAKVRLEFSNRDFILKPEMYANAEIEINAGRKLAVPDEAVLDSGLRKVVFLD
                     KGEGRFEPAEVKVGNKFDGYYEILAGLSAGERILASASFLLDSESRLKEAMGAMAGMA
                     GHGDMKGMETQAAPKAGPQEKKVQDLTLTLLTQPEKPKAGENVIRLKITNTAGQLIKD
                     AQVSFVFNMTMPGMVPSKGEGKLSQDGFYETKTMLGMAGQWEVTVIVRRPGQKEIQEK
                     FSVVAG"
     gene            complement(11260..12534)
                     /locus_tag="VF182_17530"
     CDS             complement(11260..12534)
                     /locus_tag="VF182_17530"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX6438941.1"
                     /translation="MSKFSFGSRVLIAPVLIFQLGATAIAAETPLTLAAAVKQALEKN
                     PEIQQLQHRVRGLEAKAKQALYLDDPQIAFQVGGVPLSDPTSFNQADANSIGIRQMVP
                     FFGKLGLKEKIAGGEKNIAEQEVRAKEREIISMVKMAYADLFMADRSIEILREQLEIL
                     RAVIGATEARYRVGRVTQQDVFKAQLEQSEVLNQLIVAEEEITVATVKLNTAMFRSPR
                     TPIEIPNDLETGNSIQNFSGLDDLAMASRPELRGAQQEIERAEVLYELADRNRKYPDF
                     MLGWDYMRMPTEMKKDRYAAMVNITIPFSPWTAGKRAYEVEESLAEIRAARSNREVIR
                     NMTVKEVAQAEAKVQAAKRSLQLYREGLLSQAELSFRSALAAYQTGRVEFVNLLEAQR
                     ALRESRMGYYKATASFVQNVADLERAVGRDLQ"
     gene            complement(13172..14056)
                     /locus_tag="VF182_17535"
     CDS             complement(13172..14056)
                     /locus_tag="VF182_17535"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6438942.1"
                     /translation="MVSLTSLVEKQLGRNFVIFHPSDFSAASEVAFGHALKIAMHAKA
                     KLDIMHVETQLNPEKPYWQDFPVVRATLSRWGILPEGARRDEVAKAGLRIRKILKSGA
                     DPVGTMLRHFRKLPPDLIVLATRQREGIERWLHKAVAEPLARQSGAMTLFVPQDGRGF
                     ISLTDGSVALKKILIPFDHNPPAQTALSKASLLVRGLNCAVGEFRLVHVGQRENTPVI
                     ETPHLPGWPYDILVRQGDVVDEIVKTEREWHPDLMVLATQGHVDFLDALRGSTTERVM
                     RNVRCPVLAVPVRRFSRS"
     gene            complement(14271..14600)
                     /locus_tag="VF182_17540"
     CDS             complement(14271..14600)
                     /locus_tag="VF182_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013467660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX6438943.1"
                     /translation="MATWREFAIAISIISVVPGWASAQERPYDWAWGMHPMGWMGGMW
                     GIGMMLMMLLFWFLVVAGLILAVRWFVNDHKERRSSDSALEILRQRYARGEIDKEEFE
                     NKKRDLS"
     gene            complement(14628..15047)
                     /locus_tag="VF182_17545"
     CDS             complement(14628..15047)
                     /locus_tag="VF182_17545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438944.1"
                     /translation="MKKIVMAASLSAALGLVPALAQEKREMPMKGEMPKKGEGMQGGS
                     MDMNQMKQMHGRMMEMKKGMGGMMQGKGMMKSEEMKGMGGTMGDMSEMMSDMGHMMES
                     GKMTPEQMSEMSKMMGDMSGMMKQMSERMGRGMKKAQ"
     gene            complement(15428..15562)
                     /locus_tag="VF182_17550"
                     /pseudo
     CDS             complement(15428..15562)
                     /locus_tag="VF182_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007299916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(15759..16406)
                     /locus_tag="VF182_17555"
     CDS             complement(15759..16406)
                     /locus_tag="VF182_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX6438945.1"
                     /translation="MDDCCGAKAQELTLLRGKHRNVLIAVLVINAALFVVEAAAGLLA
                     NSTALLADSSDMLGDSLVYGFSLYVLWRSATWKAKAALLKGAVMAIFGVAVLSQVIYK
                     TMYGIVPHAQTMGIIGALVLLGNGVCFLLLYRHRADDLNMRSTWLCSRNDIVANVSVL
                     LAAMGVKMFDSSWPDIVVGTAIAALFVKSAFTVLHESVSELQRLRLRSTTTAQLL"
BASE COUNT         3667 a         4801 c         4218 g         3821 t
ORIGIN      
        1 ttccacgcat tttcgatgga ccgcgagtta ggtgatcgca ctacttatcg ccggcgatca
       61 ttgatagata ataacaacgg atgcctatga agaagtttac aatgttaagc tcatcctgct
      121 tctgcgcgaa cctgctatcg agataaaatc caccgaggct aacatcggcg cccgcgccag
      181 ggagcggccg acagagccgg cgcagaatat gtacttactc aggagccaaa aggtacatgt
      241 gcgctcagta ttggaaagat gctacaagtg tgacatcata gaagctatgc cgtattcaaa
      301 caaaagcaag aaagacacca ttaccgagtg ggctgtaggc caacctctgg taatgcaaac
      361 cgagcaggag acaaccagaa aaacaaagtt gggatcagcc cagactttag ccggtgtaaa
      421 gaaaacactg gcggcaagac agaatgccgg cgcccgtaag aaggcagttt cgaaacctcg
      481 gcggaaaaag aaaagaacct cttcttagct gccccacaat ccagcttcgt ttcccgcctt
      541 cttaccttgc ctctgccgcc tatctcggat tgtgatcgct gagaacgaca tcgatcaccg
      601 tgagttttcc ttccctttct tgcagcaatg cctgctccac tgccggtttg atttcatccg
      661 gccgcgtcac ccggaagccg cgtccaccgt gcgcttgggc gtaatactgg tagtcgggat
      721 gcggcgcgat gtgggcgcca aagtgaactc cggttttttt cgccgcaccc tccgggaaat
      781 atttgatcag actggtttcc atcgacaagt agcgaccatt gttgaaaacc accacatgga
      841 tcggcaggtg atattcttca gcgaccccaa ggcaggacgg cacggcatta tagtgaaacg
      901 cgccatcgcc gattagggcg aagacgggcc ggtcaggcat cgctaatttc acgcccagcg
      961 catagcttaa accgacgccg agcccgccgg ttattcgggc aaaatatgat tgcggttggt
     1021 cgcgcggaat cgcttcctgg atgagtgtcc gataaaccgt cgtctcttcc gagatcaccg
     1081 agttatcggg cagcgcctcg gccaacacat gacacagcca tcttgggtcg atcggcgtgt
     1141 catccgcgtg cttgcgggct tcggtcttca acttgccgaa ataatcgccg tgttgttggc
     1201 gggtgcgacc gaggcgttgc tcgtaggccg cgcgattgcc tgcgacttct tcagacgctt
     1261 ttgctttggc taccattttc tccagagtct gagcgggtgg cgccacaaga gctaggtcga
     1321 cattgtagcc ccagtatggg agccgggaat tcggaaactc tgcggcaatg gcaatgactt
     1381 tggcgctctt gggcgccttg ctcgccggat accaaggggt tacagcttcc accatcagta
     1441 tcaaatcggc tgcatcgatc gccttggaat cgtgatgcaa ataaagtgga tgggtgcgcg
     1501 gaaaattcat gaatgccggc cggtaggttt ccatcaccgg gatcgaaaac aaatcacaaa
     1561 gctccacaag actctcaacc gcacgcggat cgccgccgac atattccgtg actgcaatcg
     1621 ggttcttggc gcctgcaatg agcttcagcg cctgatcgat ggccttatcc tcgagggcca
     1681 ctgagcgcac gacttcgttg gttttgccat gatcgatcca ttgaacttct tccatcatgc
     1741 attcgaacgg gataccgata agcactggac ccgccggtgg ctcaacggca atctgcaccg
     1801 cgcgttctat agtcggcgcc aacagctcgg cggaggaaat tcgatccgcc cacttgacgc
     1861 agcctctaag caaatccggt gagccaccaa gatccgtgag atcgtgcagc cactggccgc
     1921 ccggatccgc aatctgtgta ttctcgccgt aggcagcgat ctcaccgctc aaaatcacca
     1981 tcggcgtgcg ctcgtgtaag gcggcgcgca aagtcatggc cgcgtttagc ggacccgcgg
     2041 tggcgtgcag cattaccact tgcggacgat tggtcacttt tgtataaccc gcggccatag
     2101 cgacggccac agcttcgtgg cgacagttga tgtacttcgg cttttgcgct ccacccgcag
     2161 ccgcctcggc caaggcttcc cagatcggcg gccattccga tccaggagaa gaaaagatgt
     2221 aatcgatgcc gctgcgttcg atcgccttta acattgcttg tccaccgtgc atgatcaact
     2281 ccttcttgtc tcatccgggc tcgggctagt tgtctccgtc gccgatgcca ttcacgtccc
     2341 gatacatggt cggaatcgag agcgctcgct tgcttcctcg cgcaaatcta ttatcttccg
     2401 agtctttttc tcatctcgga taagaagccg ctctcctcca aagtccgtgc tgcgttgctc
     2461 atcagtaact gatccagatc cgcgtcctga actttaggat tgatgcgtat gagcaggcgt
     2521 tggattcgtt tccaagcctc gccgttgggg gcgatatcca aagttggcat ctgtaaacga
     2581 gtgactttgt atgacgcttc gatatcttcg tctcttgtca ggcggagatg cttcttgaga
     2641 atctcagaca catcacgctt gttcgccggc tcttgaatat aaaccagcgc ttcgacaatt
     2701 cctttggtca atgcgatcat cgtttcgtta ctaatggcgg ctgaatcttt ccgggcacac
     2761 atcgctatac cctggtaaat gagctttacc gtcgcggcgt ccgcgagcga gcgtgcgccg
     2821 gctctcttgg ctatatccgc ataactgtaa ggcactactg ccgcgtcgat attgccagcg
     2881 ctcagggctt gcgtcacggt gccggtgtcg ccaatcaccc gcatattgag tttgtatttg
     2941 tcgggatcga tgtccaggcc gttgagcaca gccatggtcg ccaaccagaa gccgccgccg
     3001 atgctttgca cgccgaaggt tttgccgcgc aaatcctcca gtgattctat tcccttacgg
     3061 gcgatcaact cgcgggggat ggtatcgttg ccaactgcaa agcaaccgag tttcattcct
     3121 cccgtcgccg cgctaatggc gctggttggc gcagcccaaa ccagagaggc tccgccggac
     3181 gccaacgtag ccatcatgat cgggccgctg cgggtctgca atactcgcgg atcgacaccg
     3241 tgctttctga acaaacctcg atcaacaccc acccacatcg gcgccatagt ctcattatga
     3301 ccgccgtaag cgaccagcag ttcgcccgag gccgggctcg cctcatgcgt caaaaaaatc
     3361 ccgatgaata agatcggagc gaaaaatacc cgcaatcgaa tcgccatact cacatcggcc
     3421 tcatctttcg ctgctattag cacagcactg tccttttgcc cagcccgaat tctagagaag
     3481 ccgtgctcgt ggctacatgt tcgtgttgga acggatcgca atgaatgcca catccttttt
     3541 tgcatcgcat cggttattcc tggaaggcgg aaacaaatta caatgcaatt cgtcgcaatc
     3601 tcagcgcatt tgcaatcacg gaaactgagc tcaacgtcat cgcggcgctg gcaatgatcg
     3661 ggctgagaag aataccgaaa aacggataga gaactcccgc ggcaatcgga acgcctagaa
     3721 cgttgtagaa gaaagcgaag aaaaggttct gtcggatgtt gcgcatcgtt gcccggctca
     3781 ggcggcgcgc tttggcgatg ccgcgaagat cgcctttgac cagtgttata tgagcgctct
     3841 caatcgccac atcggttccg gttcccatgg cgatgccgac atgcgcttgc gccagggcgg
     3901 gagcatcgtt gacgccatcg cccgccatgc cgacgacccg gccttcggcc tgcagccgtt
     3961 tgatcacttc ggccttctgt tcgggcaaca cttcggcttc aatccgatcg atctccaacc
     4021 gtcgcgcgac cgcttcagcc gtggtccggc tatctcccgt cagcatgacg atcctgacat
     4081 tctctccatg cagaattttt atcgcttcgg gggttgactg cttgattggg tcggaaaccg
     4141 ctaccagtcc agccgcccga tcgtcgatcg cgacaaacat caccgtatgt cctccgctgc
     4201 gaagttcctc ggctcgtgcg cgtagagggc cagggtccgt cgacagctct tcgagaagct
     4261 tgatattgcc caatgctatc gaacggcctt cgactttgcc gataactccc ctgccggtca
     4321 gggagcggaa ctcctcagta ttcccgagcc tcgctccctc tttctcggct gccgcgacaa
     4381 ttgcggcggc gagcggatgc tcgctcgcgc gctcgatgct cgccgcgagg cgaaggagct
     4441 ctttgcgatc cgaacccgaa ttcatggtaa taatttccga cagctgcggc tttccttcgg
     4501 ttaatgtacc ggtcttgtcg acaaccaacg tgtttacttt ctccaggatt tccagcgcct
     4561 cggcattctt gatcaggatg ccttcggccg cgccgcggcc gacaccgacc atgatcgaca
     4621 tcggtgtcgc caagcccaac gcgcaaggac atgctatgat cagcaccgat accgcattga
     4681 cgatggcata ggccatgcgc ggctctggcc caaagaacgc ccatacggca aacgtcactc
     4741 cggctacgat aagtacgatt gtaacgaaat aagcagagac gatatctacc aggcgctgga
     4801 tcggcgcgcg cgtgcgctgt gcttcgctta ccatgcggac gatttgggcc agcaaagtct
     4861 cgctgccgac tcgctgcgcc tccatcacga cactgccggt tccattcacc gttccgccgg
     4921 tcactttgct tcctttagat ttttccaccg ggattggctc gccggtgatc atcgattcgt
     4981 cgattgaagt tgcgccctcg atgacaacgc cgtcaaccgg aactctctcg ccgggccgaa
     5041 ctcgcaggcg atcgccggct ttgatgaggt ctaacgaaac atcctcttct ctgccgtcct
     5101 ggtaaagcaa tctggcggtc ttcggcgaca gcccgagaag tgctttgatc gcgctactag
     5161 tttggctgcg cgcgtgcagc tccagcactt gacccaaaag caccagggtc gtaataaccg
     5221 cggccgcctc gaagtaaacc gcgacagttc cgctgtggcc gtaaaatgat tcgggaaaaa
     5281 tgtccgggaa aagagttgcc gcgacgctat acagataggc cgcaccgacg ccgatggcga
     5341 tcaacgtaaa catgttcagg tttctgttta cgattgaata ccagccccgt tggataaacg
     5401 gccagccgcc ccagagcacc acgggtgtgg ccaacacgaa ttgaagccag gtcaacaaac
     5461 ggggagatag cacgtgctgc aacggctgtc cggagatcat atcggacatc gcgacgatga
     5521 acagcggcag ggaaagcgcg gcgctgatcc agaagcgcct gatcatatcg actagttcag
     5581 gattcggcgc atcctcaaga gttacggtct tcggttcgag cgccatcccg cagatcgggc
     5641 aagaacccgg ctccgaccgg acgatctcgg gatgcatcgg gcagacgtat tcggttcgtg
     5701 ttgccggcgc ggcaagtgtt tccggctcga gcgccatgcc gcacttcgag catgcgcctg
     5761 gcttggacgc gcgcacctcg ggatccatcg ggcagacata caccggctca gcaggtttcg
     5821 ctttcggagg ggtttgcgcg ccgccaggtc tgtgatcgtt atcgtggcca gcgtggccgg
     5881 cgtgctttgc gttggagtac ttctcaggat cttctttgaa tttctgtgcg caatgatggc
     5941 tgcaaaagta gtaaaccatc ccattgtact cgtgctttgc cgccgcggtg cccggctcca
     6001 cggtcatacc acagactgga tcggcaacgg atttgttttg cgcactgccc tgcacgacac
     6061 tcgacgcgaa cctggccggg tcgttgttga atttcgccaa gcaatgtttg ctgcaaaagt
     6121 aataagtttg tccattgtgc tcgtgttttc cggcagaacg agctggatcg atggtcattc
     6181 cgcagatagg gtcaatagcc ataatcatct cctcgaatcc ttactgtcag ccaggctgac
     6241 gcaggaccgg cgctacgatt acacctgcat gtaaactgct gattcaaaaa ccgtccagca
     6301 gagttaagga cagcccggaa gaaagacttt catcatagat cggccgtgac aaccgggcac
     6361 tcgcggaacg atttttcaag ctttctgaat tgcccggcgc attttccaac ctctccagat
     6421 gtcgaaaatc gctggataga cgagaagctc gaggaggaag gaactgatga cgcctccgat
     6481 catcggcgcg gcaattcgct tcatcacgtc ggccccggcg ccgtggctcc acatgatcgg
     6541 aagcaatccc atcaggatgg ccataaccgt catcaccttc ggccgaatcc gcttcaccgc
     6601 gccttcgata atcgcttcgt ggagatcgtt gatgttgcgc atgcgcccct cactacgcca
     6661 cttgtcatat gcatgctcga ggtacagtag catgatgact ccggtctccg cgtccacgcc
     6721 agccagcgca atgatcccga cccagacggc gacgctcatg ttgtattcca gcaagtaaag
     6781 tagccagatc gctccgatca tcgaaaaagg aaccgccagg agcacgattg cgcacctgcc
     6841 caccgattta aagttgatgt aaagcaacag gaaaataacc agcagagtga gaggcacaac
     6901 gtaaatcaat ttctgttgag cgcgctgcat gtattcatac tggccgctcc aggaaagagt
     6961 gtatccggtg ggaagtgtga cttgctcggc gactttcttt tttgcttctt ccacatagcc
     7021 gccgatgtcc ctgccggtca tatcgacata aacatagccc gaaagctggc cgtcctcatc
     7081 tcggatcatc gccgggccgg aagttaaccg caggtcagcc aagtggccga tcggcacctg
     7141 cgccccattc atcccgggga tgagtatccg cttgagtctt tccgggtcgt cgcgcagctc
     7201 ccgggcgtag cggacgctta ccggatagcg ttctctgccc tcaaccgtgg tcgtaatgct
     7261 ctcaccgccg atgacggatt cgataagcat ctgaacctcg tctatagcca gtccataccg
     7321 agcgatctcc gtgcgtctca actcgaaatc gaggtaataa ccggccgtga ccctctccgc
     7381 gaataccgtt cgtgtgcccg ggatcatttg aagcgccgcc tccagattct tgccgatctc
     7441 ttcgattttg cccaaatcgg ggccgaaaat tttaatgccg atcggcgtac ggattccggt
     7501 ggagagcata tcgatgcggt ttttgatggg catcgtccag gcattggcca cacccggaag
     7561 ccgcagcttc ttgtccatat ccgccaccag ctcgtccctt gtgatggtgc gcgtggttcc
     7621 aaacaggact tcgccgatgg gccggagagg gccgggccaa tcggaacgat caaccggcaa
     7681 ttgccgccat tcctgctccg gctttaacat cacaagggtt tcgcccatgc tgaagggcgc
     7741 cggatcggtg gagctttccg cgcggcctgc tttgccgaga acccattgga cctcagggaa
     7801 gcttttcaaa atacggtctt gcatctgcag caaccgcgct gactgggtta tcgatattcc
     7861 cggcaaggtt gtcggcatgt aaaagatcat tccttcgttc atcggcggca tgaactcaga
     7921 acccaaacgc atgaaggcgg ggatcgtcaa gagcaacgcc agcaccgcca gcgcgatggt
     7981 ggtctttcta aatcggagaa cccaatgaat caccggcatg tagagcgcga tgagaaaacg
     8041 gttgacggga tttctggctt ccggacgaac acgaccgcgt acgagcagca gcaaaaggac
     8101 cggagccaag gttatagata ggaatgccgc gaagaacatc gcgaacgtct tcgtataggc
     8161 cagcggtttg aagagcctgc cttcctgccc ctcgagtgta aaaatcggca ggaacgagac
     8221 ggtaatgatg agcagagaaa agaacaaggg ctttccgacc tctttcgctg cctcgatgag
     8281 cacgtcggtt ctatttcccg gccgtccctg ctcttcccaa agttccagct tcttatgcgc
     8341 gttttcgacc atgataatgg acgcgtctac catcgcgccg atcgcgatcg cgataccgcc
     8401 gagcgacatg atattggccg taaggttcag tgaatgcatc gcaatgaacg agatgatgat
     8461 cgcgatcgga agcgtgatga tcacgaccag ggcgctcggc agatggaaga gaaacacgat
     8521 gatgacgaca ctgacgatca agctttcctc gatcaacttt tccttgaggg tggcaatcga
     8581 tctgagaatc agatccgaac ggtcgtacgc ggtgacgatc ttgacgcccg gaggcaaaga
     8641 gggctcgatc tctttgattt tttccttgac cctttcgatc aacctcagcg cgttttcgcc
     8701 gtagcgcatg accaccacac cgctgacaat ttctcccctc ccgtttagct cggctatgcc
     8761 cctccgcata tcaggcccca gcgtgaccga ggcgatatcc cgcaccagaa tgggtgttcc
     8821 ccgttcgccg cccactgcaa tagcttcgat gtcctgagtt gatcggatgt aaccccggcc
     8881 tctaatcatg tactcggcgc cgcttagttc tatcgagcgg ccgccgactt cgttgttgct
     8941 catgcgaatc gcctcgacga cggttttcgg ggacaggcca taacctgcta aacgattcgg
     9001 gtcgatattg acctgatact ggcggacaaa tccgccgaga cttgccactt ccgcgacgcc
     9061 ttccacgctg gcgatctggt agcggagata ccaatcctgc aggctgcgca gttgcgccag
     9121 atcgtatttg ccggtttcat cgaccagagc gtattgaaaa acccatccga cgccgcttgc
     9181 atccggcccg agggtcggag ctacaccttt gggcagcctg ccgctcacct gattcaaata
     9241 ttccagtaca cggcttcggg cccagtaaat gtccgtgccg tcctcgaata tgatgtagac
     9301 ataggagaag ccgaagtcgg aaaatcctcg gacgaacttc actttgggag ccgaaatcat
     9361 cgtcgtcaca atcgggtagg tgacctggtc ttccatgatc gtcggatttc gtcccatcca
     9421 tggtgtatag acgatcacct gaacgtcgga gaggtcgggt atggcgtcca acggcgtgtt
     9481 ctttaacgcc caaatgccca tggcactcaa aaaaatcacc atcagaaaaa tgagaaattt
     9541 atttcgagca ctggcttcaa tgattctttc gatcataaat ttcgctcgta tcgtttaggg
     9601 cttctggctt gtagctcgct cgcagcgctg cctctagcac tatgccaagg gcgataagct
     9661 atgtgccgct ccggctagcc ggcgacaacg ctaaactttt cctgaatttc tttctgtcct
     9721 gggcgccgta cgatgacggt cacttcccat tgtccggcca tacccagcat tgttttggtc
     9781 tcgtagaatc catcttgtga cagcttccct tctcctttgg acggaaccat tccgggcatc
     9841 gtcatgttga agacaaatga gacttgagcg tctttaatca gttgcccggc ggtattggtg
     9901 atctttagcc ggatgacatt ttctcccgct ttgggcttct caggctgggt gagtaaggtt
     9961 aacgtaaggt cctgtacttt cttttcctgc ggaccggctt tcggcgctgc ttgcgtctcc
    10021 atccccttca tatctccgtg acccgccatc ccggccatag cgcccatggc ctctttaagg
    10081 cggctttccg aatctaacaa gaagctggcg ctcgcgagaa tgcgttctcc ggcagagagt
    10141 cccgcaagga tttcatagta accgtcgaat ttgttgccga ctttcacttc agccggctca
    10201 aaccggcctt cgcctttgtc cagaaatacg acttttctca ggccggaatc caaaaccgct
    10261 tcgtcaggca cggccagctt ccgaccggcg ttgatttcta tttctgcatt cgcatacatc
    10321 tccggcttta aaatgaagtc gcggtttgaa aactccaacc ggacctttgc ggtccgagtt
    10381 ttttcttcga gaacgggaga gatccatgag accttgccga caaaatcccg cgctggatag
    10441 gaagcgacgt tcatctttgc cttttgtccg acgcgaatat gagcgagctc atattcatag
    10501 atatccgcat taacccagac ggtggataaa tcagtgatcg tatagagctc cttatccggc
    10561 atgaccctca tcccttgaac agcgtccttc ttgatcacaa aaccgctgat cggcgcgaag
    10621 aaggttagcg ttctctgagg tttgccggtt ttctccagat tgttgatctg ctcctctgaa
    10681 atatcccata ggctaagccg gcggtgcgcc gattccaaaa gtgaatcgga tcctgcactg
    10741 atttctttta tggagctcga tccgagcgtt tgtttcgctt tgagagccag aagatattct
    10801 tcctgggtgg ctaccaggtc cgggctgtac aaagtgaaca gaggctgccc tttgcggacg
    10861 agagtaccgg tgtaattgac cagaagttgt tcaatccagc cttcaacttt gctgaacacc
    10921 tgagcgatcc gcgtctcatc ataggtgacg atgccaacag tccgaatttt ctgcatcagc
    10981 ggccgaattt cggcggttgc cgttcgtatg cccacgagtt gctgcatttc agaacttacg
    11041 ttgacgccgc ccgaactcat atcatgccct tgcatcgatt gactgtcggt ggacgcttcc
    11101 gttgcggaat gaccggacaa cgcgtgatct gctttcggat cgctctgccc gcgccaatag
    11161 ccaatcgcaa atactcccgc aagcagaacc agagctccca aaatctttat aattttcata
    11221 ttgatctccg gataaactta tctgagcctt attagtaaac tactgcaggt ctctgccgac
    11281 cgcgcgttcg agatcggcta cgttttgcac aaaactagcc gtggccttgt aataccccat
    11341 gcgggattcg cgcagcgctc gttgagcctc gagcagattt acaaactcca cccgccccgt
    11401 ttgataagcg gcgagagcgg atctgaagga cagctcggcc tgagacaaga gaccctcacg
    11461 gtagagctgg agggagcgct ttgccgcctg gactttcgcc tccgcttgcg cgacttcctt
    11521 cacggtcatg ttcctgatca cctcacggtt ggatctcgca gcgcgaatct ctgccaggct
    11581 ctcttcaact tcgtaggcac gtttgccggc ggtccaggga gaaaagggaa tggtgatgtt
    11641 caccatcgcg gcatagcggt cctttttcat ctccgtgggc atccgcatat aatcccaccc
    11701 aagcatgaaa tccgggtatt tccggttgcg gtccgccaat tcgtacaaca cttcggctcg
    11761 ctcgatctct tgctgcgcgc cgcgcaactc cggccggctc gccattgcca aatcatccaa
    11821 tccggagaaa ttctggatgc tattaccggt ttcgagatcg ttgggaatct caatcggtgt
    11881 acgcggcgag cggaacattg ccgtgttcag cttgacggtc gcgacagtga tttcttcctc
    11941 cgcgactatt aactggttca gaacttcact ttgctcgagc tgcgctttga agacatcctg
    12001 ttgagtcacc ctacccaccc ggtagcgtgc ctcggtggcg ccgatcacgg cgcgcaagat
    12061 ctcgagctgc tcgcgcagaa tttcaatcga tctatccgcc atgaagagat cggcataagc
    12121 catttttacc atcgatatga tttcccgctc tttggcgcgc acctcctgtt cggcaatgtt
    12181 tttttctcct ccggcaattt tttctttgag gccaagcttg ccgaagaacg gaaccatttg
    12241 gcgaatgcca atcgagttcg catccgcctg attgaaactg gtcgggtctg ataacggcac
    12301 cccgcccacc tggaacgcga tttgtggatc atccagatac agtgcctgct tcgctttggc
    12361 ctcgagaccc cggacccggt gctgcagctg ttgaatttcg gggttttttt caagcgcttg
    12421 cttaaccgct gccgccaagg tcaggggcgt ttcagcagcg atggcggttg caccgagctg
    12481 gaaaatcagc acaggcgcaa tcaacactct ggaaccaaaa gaaaacttgc tcatcattcc
    12541 tccgttcaga tcgatcaaca caaatgtcgg acacataccc gcggtgctcg gcctggcagc
    12601 gcgtaaacag atgccgtgag acgtaagtcc ggtattgctc ggagggcaac caacggcgct
    12661 ttaaatgagc atgctaagca cgtgtcactc gttagcgatc ccggaagcac gccggccaac
    12721 gtgagggaga ttttagatgc gcagattgat tttcagagaa gggatcggag ggctcgcggt
    12781 agacaagtgt gtcgtcctga gacgaagcga aaaatcgaat gcagccgaga ggacatgtaa
    12841 cggcagtaat aacgcatgta cggttacgat gtgcgaaaat ttaacttcgg aagtcggcag
    12901 aaattcagaa aacgccgcgc ggcaattaga ttcgggcttc tgcttgccgc tgtcgtgaca
    12961 gtcatgagct tcactcgcag cggctgtttg caaacccagc gtcggacgcg ctcgcccagg
    13021 acacaccgtg catagagcgt agaacgctag aagcattacg gaaataattt tataccgacg
    13081 cgacatgtta ccctgcgcgc agtctaaagc cgtgatattc acgtgtcaac ttcatgggac
    13141 tgataacttc atcccggtat gcttcttgcg ctcaagaccg gctaaatctc cgaaccggaa
    13201 cggcaaggac gggacagcgc acgttccgca ttactctttc cgtcgtgctc ccgcgcaaag
    13261 cgtcgaggaa atcgacatgt ccctgggttg cgagcaccat cagatccgga tgccactcac
    13321 gctcagtttt tacgatttcg tctactacat cgccttgtct taccaggata tcatacggcc
    13381 agccgggcaa gtgaggcgtt tcaatcaccg gtgtgttttc cctctggccg acgtgaacga
    13441 gccggaattc tcccacggcg caattaaggc cccgcaccag aagtgaggcc ttgctaagcg
    13501 cggtttgtgc aggaggattg tgatcgaaag ggatgaggat tttttttaat gcaacgctac
    13561 cgtcagtaag cgaaataaac cctctcccgt cctgcggcac aaaaagcgtc atggctcccg
    13621 attggcgcgc gagtggctct gcaaccgctt tatgcaacca ccgttcaatg ccttccctct
    13681 gacgggtagc gagaacgatc aagtcgggcg gaagtttgcg gaaatgccgc agcatggttc
    13741 cgaccggatc agctcctgac tttaggatct tcctgatacg gagcccggcc ttggcaacct
    13801 catcccgccg agcaccctcc ggcaaaatac cccagcggct caatgtggct cgcacaaccg
    13861 ggaagtcctg ccagtatggc ttctccgggt taagctgtgt ctctacgtgc atgatgtcga
    13921 gctttgcctt tgcgtgcatg gcgattttaa gcgcatgacc aaaagcaact tcgcttgccg
    13981 cagaaaaatc cgacggatga aagatgacaa aatttcgacc cagctgcttt tcaacaagag
    14041 aggttagact caccatatag ccctaagctt tttctatccg cgtacgaggt cacctgcagc
    14101 aacaggagaa gcaggacctg cacgatgagg gcgctgcatt ctgtccagag aaatagacgg
    14161 caatcgccag aaagtcgtcg aaacaagctg ggttgagatt gccttgatcc atttgcatgc
    14221 ggatttctcc ggtatttgta gtattcttcg ccacttcgaa ctgagcacca tcaactgaga
    14281 tcccttttct tgttttcaaa ctcttcttta tcaatctcgc cgcgcgcata gcgctgacgc
    14341 aaaatttcta atgctgaatc gctgctccgc cgttccttgt gatcattcac gaaccaccgg
    14401 accgcgagta tcaaacccgc aacgacgaga aaccagaaaa gcaacatcat aagcatcata
    14461 ccgatgcccc acatgcctcc catccatccc atcgggtgca ttccccaggc ccagtcatac
    14521 gggcgctcct gagcagaagc ccatccaggc acaacagaaa tgatcgagat cgcgattgcg
    14581 aactcgcgcc aggtagccat ctgaacctcc tcctttgcca gccaagatta ctgcgccttt
    14641 ttcattcccc gccccatccg ctctgacatc tgcttcatca tgcccgacat atcgcccatc
    14701 atcttcgaca tctccgacat ctgttcgggc gtcattttgc cgctttccat catgtggccc
    14761 atgtcgctca tcatctcgga catatcgccc attgtgcctc ccatgccttt catctcttcg
    14821 ctcttcatca tccccttgcc ttgcatcatg ccgcccatgc cctttttcat ctccatcatt
    14881 ctaccgtgca tctgtttcat ctggttcata tccatgctgc cgccctgcat cccctcgccc
    14941 ttcttcggca tctcgccttt catgggcatc tccctttttt cctgcgctag cgcgggaaca
    15001 agacccagcg ccgctgatag actcgcggcc ataacaattt ttttcatgat atcctcccat
    15061 tttttgttag atttacgttg acttttattg atcgagtttc caaacaaacg ttctctactc
    15121 tattccggca acgtccttta tgaccagtcc aaaccgcttc gtctcggggg atattttggc
    15181 gaaggagaca attgtctcgc gatgatttcc gctcccttta ttttccagcg caaccggggc
    15241 atagctgttc ccggcatcat cacgcagtac aattgtcgtc ttaaggttta tagcagcgcc
    15301 gaaatcttac ggactccgtc cacaaccatt ctacctgggg tatcggattc gcaatgatcg
    15361 gactgctctt gtcggttctg gcaggtcgtg aaacccacgg ccatgcacgc tatgatgcga
    15421 aacaccatag agcggcgtaa ccggcaaact catttagatc catcgtgagc ccgcggcgct
    15481 ctggcccggc gagatcgatc tccatgatta cggtagtaga ccacgttaaa cctgctcccc
    15541 gaagcgaatt cgccgcaagc acctcgattc cacgaggctg cccacgtaat aaaaagggat
    15601 cggggatgcg ataaaccaac cgccgacgag gaccttcgcg gccgatagaa tccgggacta
    15661 ggccggggac accctttaaa tatttagatc aacatagccg aggcgcaatc ggatctgcta
    15721 acggaaagtg gcggcgaaac ccaagccaat gtttttcgtt acaataattg tgctgtagtt
    15781 gtggatcgca atctcaatcg ttgcagctcc gaaacagatt cgtggagaac ggtgaaagca
    15841 cttttcacga acaaagcagc gatggcggtc ccgaccacga tatccggcca gctcgaatca
    15901 aacattttaa cgcccattgc ggctagtaac actgatacat tagcgacgat gtcgttgcgt
    15961 gagcacagcc aagtggagcg catgttgaga tcgtcggcgc gatggcgata gagcagcagg
    16021 aagcagaccc cattgccgag caacaccaac gctccgataa ttcccatcgt ctgagcgtga
    16081 ggcacaatgc cgtacattgt cttgtaaatc acttgcgaga gaacagccac tccgaatatc
    16141 gccatgaccg cgcccttcaa tagcgcagct ttcgctttcc acgtcgcgct gcgccaaagg
    16201 acataaaggc taaagccata aactaacgag tcgcccagca tgtccgaaga gtcggcgaga
    16261 agagccgtcg agtttgccag caagccggcg gcggcttcga caacaaagag agcagcattg
    16321 atcaccagta cggcgattaa gacgtttctg tgcttaccac gtaagagagt tagttcttgt
    16381 gctttcgcac cgcaacagtc gtccataaca acagtcttat cagtgcactc gcaagcgcgc
    16441 cagggtagcc gttaaccaag ttacaaagca ttcggccaat gacataaaat ccaccaaaat
    16501 ccccaca
//
LOCUS       DASUVS010000328        12087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_136926, whole genome shotgun sequence.
ACCESSION   DASUVS010000328 DASUVS010000000
VERSION     DASUVS010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12087
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136926"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..874)
                     /gene="pstB"
                     /locus_tag="VF182_17560"
     CDS             complement(92..874)
                     /gene="pstB"
                     /locus_tag="VF182_17560"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX6438946.1"
                     /translation="MRKMNLAGAPHPIIQIKDLSFYYGPKPALKSINVDISEKKATAF
                     IGPSGCGKTTLLRCLNRMNDLIADVRVTGSITLDGNDIYDATTDVIQLRRRVGMIFQK
                     WNPFPKSIYENVVYGLRIVGIKDRQYLDEVVESTLKRAALWEEVKDTLHKSAAELSGG
                     QQQRLCIARALAVEPDVLLMDEPCSALDPISTAKIEELIHQLKDAFTIVIVTHNMQQA
                     ARVSDYTAFFYLGELVEYGETGKLFTSPDKQQTEDYITGRFG"
     gene            complement(978..1883)
                     /gene="pstA"
                     /locus_tag="VF182_17565"
     CDS             complement(978..1883)
                     /gene="pstA"
                     /locus_tag="VF182_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX6438947.1"
                     /translation="MNLKARIARNETVDRLFVLFGLLATFVGLLTLAALMLDLGIDGA
                     GRLDWQFFTSFPSRFASRAGILSAWVGTILVMIVTAFTAVPLGVAAGVYLEEYAPKNW
                     LTAIIEINIANLAGVPSIVYGLMALGLFVYQLGLGQSILTAGLTLALLILPIVVVATR
                     EAVRAVPGSMREAALALGATKWQTVKDHVVPYSTGGILTGVIIALSRAIGETAPLITI
                     GALTFIAFLPGAPIKAEFPFISFEWLMSPFTVMPIQMFNWVSRPQEEFHLNAAATGLV
                     LMFMTLAMNAVAIYLRYRFRKRIKW"
     gene            complement(1895..2782)
                     /gene="pstC"
                     /locus_tag="VF182_17570"
     CDS             complement(1895..2782)
                     /gene="pstC"
                     /locus_tag="VF182_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX6438948.1"
                     /translation="MIRGLRRASERVIEFFLFLAALSSVLITVGIVGILVFESLQFFK
                     DVSILEFLTDTQWTVLFADPHYGIMPLVSGTLVTSFVALVVSLPLGTIIAIYLSEYAG
                     SRTREILKPTLELLSAVPTVVFGYFALLFVTPLLKRVLPNLPGFNMLSAGMVIGIMII
                     PYVSSLSEDAMRVVPVHIREGSYAMGATRLQTALRVLFPAAFSGIASAYVLGFSRAVG
                     ETMIVAVAAGMQPNLTLNPMEGAATITAYIVQVSLGDLPHGSIAYQSIFAAGLTLMLM
                     TLIFNIGGYLLRKRFREIY"
     gene            complement(2913..3902)
                     /locus_tag="VF182_17575"
     CDS             complement(2913..3902)
                     /locus_tag="VF182_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6438949.1"
                     /translation="MSKLVTSFVFAAFLAALPQAATAQVIQIDGSSTVFPVTEAVAEE
                     FQKSKKGKVKVTVGISGTGGGFKKFCRGETDISDASRPILKQEIEACKGSGVEFFELP
                     VAYDALTVIVNPKNDWAASMTVAELKKIWEPAAQGKVTNWNQVRPNWPDAPLKLFGAG
                     ADSGTFDYFTEAIVGKAKSSRGDFTASEDDNVLVQGVANDRNALGFFGFAYYIENQKK
                     LKAVAIDGGKGPVAPSAKTVEDGTYQPLSRPIFIYVSKKAIAKPEVKEFIEFYLKNAA
                     ALVKEVKYVALPVKAYTLALEHIQKGRTDTVFGGQAEVGVKIEDLLKRESKSN"
     gene            complement(4283..5164)
                     /locus_tag="VF182_17580"
     CDS             complement(4283..5164)
                     /locus_tag="VF182_17580"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX6438950.1"
                     /translation="MSRTRLKKGVSIAVRLLVDDDSKDIHDLRVSSRRLQQVISLLFP
                     KPRHGKSRKVIRSLRKLRRDWSACRNFDVNLVLLEEKLESITADGARDAWDYVRRCLL
                     ELRAQEFDRAKKRLRNLDILSFIRRTEQLFDTVKTEKLQNLDSREIFADTLTDWDEAI
                     VQAKSNPDSKHLHEMRISGKRLRYRLEVLAELDNGTAEYVNSLKALQDELGRWHDWHV
                     LLHFVTDLSEKPEFVRDHPELQQILTRDVAEEACKNNERFDPILKQAEMLQLKLGSFK
                     DHFKASPEAQEPVSSSL"
     gene            complement(5247..5756)
                     /gene="sixA"
                     /locus_tag="VF182_17585"
     CDS             complement(5247..5756)
                     /gene="sixA"
                     /locus_tag="VF182_17585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEX6438951.1"
                     /translation="MNLYLLRHAVAATKGDPAFASDSERPLTNKGIKKFRKAARGIER
                     LAITFDMILSSPLVRARQTADIVAKVLNNESCVEEIAELAPESLPEQLTSALHDINDK
                     EHVMLVGHEPFLGKLAFFLLNGKSNSDNSILLKKGGICRIEINAVPPTEPGQMHWLLT
                     PKQLRLMAR"
     gene            complement(6182..7708)
                     /locus_tag="VF182_17590"
     CDS             complement(6182..7708)
                     /locus_tag="VF182_17590"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX6438952.1"
                     /translation="MADYFAVIDVGSNALKFQVALVDQPKRYRVLEQDRQPIRLGHNV
                     FRTGHLDPATAEAALKALADFKSVAERYQVKAIQAVGTSALREACDGKSFLKEAHQLG
                     VPMQILSEEDEARLISLGIMSGLRFYLPLGLFLDIGGGSIELAVANTTNIFCLFSLPL
                     GAVRLTEELFTADPPRKREIRALRQFVRQQLRSVGQRVGQEKFTMAFGSGGTMTSLAE
                     TNLRVAGDGKNGSLAILRRTRLKALLDLLTTIPVKQRASLISGDPKRADIIVAGGLVL
                     YEIMSEIGLDYIFVSRRGLRDGLMIDLLQRHYDKSGVWDPDAERAESLEQVCQKYLYD
                     APHAYHVSQLALNLFYQLNNLHKLTDKYASLLQAAAMLHDIGLFIAEPKHHKHSYYLI
                     KSSEMNAFDKLNLDVVANIARYHRKSHPSQKHLGFSQLSPANQDVVRKLSALLRIADA
                     FDYRHDQKVTALTCAKKSKAITITASGTGNVGDEVARALYKGELFEEVFNHRLIIRKA
                     "
     gene            7941..10025
                     /gene="ppk1"
                     /locus_tag="VF182_17595"
     CDS             7941..10025
                     /gene="ppk1"
                     /locus_tag="VF182_17595"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX6438953.1"
                     /translation="MDGDKNHRYLQRELSWLEFNRRVLEEAKDETVPLLERVKFLAIF
                     SSNLDEFFMVRVARIKRQLFKGAQHPTPDGLTPAEILTAISKRAHELVEEQHLCFIDI
                     ILPRLATEGIQLVRPQEMSGEQERFLEDFFNRTLYPILTPLAIDPGHPFPYLANRSLC
                     FVVSLSPTIASRLPHTDLSILHIPTHVVSRFISLPTREGQHAFVLLEDVLSQYVPRLY
                     HGFEIRSCHAIRVTRDAEFGMARRHDDDLLMTIEQGVRERRMGDAVRLQYDPELPQDF
                     VSQLVDELELGAEDLYPSTGFTAFTDLFQLYSAVNMPRLKDRPQTALPVAAFERAGDI
                     WSAIRAGDIMLFHPYHSFDAVTRFVDEAAKDPKVLAIKMTLYRVSPASPIAQALARAA
                     ECGKEVSVLVELQARFDEEANITWARALEEVGAHVVYGLVGYKTHCKICLVVRQEIDG
                     IRRYCHLATGNYNVRTAGVYSDLGLFTCREAFGQDLTELFNLLTGYTRPQRFQQLLLA
                     PTGLREHFINCIRKEKEHAHAGESARIIAKVNGLTDPAIIDELYSASQAGVQIDLIVR
                     GMCSLSPGVAGVSENIRVISIIDRYLEHARIFYFYNRGESTHWLASADWMPRNFDRRV
                     EIAFPILDLQHQLRLKEILEIQLSDTAKAWQTQPDGSSLRIANADRPFIRSQDRLYEI
                     LRAENDSKKEVI"
     gene            10543..11448
                     /locus_tag="VF182_17600"
     CDS             10543..11448
                     /locus_tag="VF182_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEX6438954.1"
                     /translation="MEIDTMIESARELIKPFRVTDRQSFRLKDFDPADTLHLNSEDKP
                     RAKEGLAAGIEVLAKLQDKLYAQNRWAVLVIFQAMDTAGKDSVIKHVMSGVNPQGCQV
                     HSFKAPSGEELDHDYLWRCARRLPERGMIGLFNRSYYEETLVVRVHPELLEKQKLPPE
                     LVTKNIWKERFQDIRKFERYLTRNGVVIRKFYLHLSKKEQKKRFIERIDKPEKNWKFS
                     VNDVHEREHWDGYMEAYEDMIRHTSTKQAPWYIVPADNKWFTRLIVAAALVDLLVSLD
                     LNYPKLSKEELKQLDEAKERLLTVK"
     gene            complement(11460..11981)
                     /locus_tag="VF182_17605"
     CDS             complement(11460..11981)
                     /locus_tag="VF182_17605"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HEX6438955.1"
                     /translation="MRLWQHFDALTFFNRVMDLFFKLLIFFVVVVLGMGLVRLFWETW
                     RIMAVSQLKDAFSFTVTNLLTFFIILELFKSLVEYFREHRLRLTFIVDATLVFILREV
                     MISLYQHQSPPLLIMALAFLALVLGVLRTLAIVYSPMERKMFEHFVETKQNAQHADEK
                     IARRSETASLFPF"
BASE COUNT         2954 a         3102 c         3086 g         2945 t
ORIGIN      
        1 taagccgccc atgtagagaa tttcttcacg cagcttcttt aactcttcct cgtatctttt
       61 gtcggtatgt tccgtctgca tggctgctcc attaaccgaa ccggccggtg atgtaatcct
      121 cggtctgctg tttgtccggg ctagtgaaaa gcttgccggt ttcgccgtat tcaaccaatt
      181 caccaagata gaaaaaggcc gtgtaatcgg aaacgcgcgc cgcctgctgc atgttatggg
      241 tgactatgac gatcgtaaag gcgtccttga gctgatgaat aagctcctcg atcttggctg
      301 tcgagatcgg atccagggcg gagcaaggct cgtccatcag caagacgtcg ggttcgacgg
      361 cgagcgctct tgcgatgcaa agccgctgtt gctgcccgcc cgaaagctca gcagcgcttt
      421 tgtgaagagt gtctttaact tcctcccaga gcgccgcccg cttcaaggtg ctctcgacca
      481 cctcatccaa atactgacgg tcctttatgc cgacgatgcg caggccatag actacgtttt
      541 cgtagatact tttgggaaat ggattccact tctggaaaat catcccgacc cgccgacgca
      601 actgaatcac gtcagtcgtg gcatcataaa tgtcgttgcc atccaaagtg atgctgccgg
      661 tgactcgaac atcggcaatg agatcgttca tgcgatttaa gcaacgcagc agggtcgttt
      721 tgccgcagcc cgacgggccg ataaacgccg tcgccttttt ctcagagatg tcgacattaa
      781 tcgactttag cgcaggtttt ggcccatagt agaaactcag gtcttttatc tgaatgatcg
      841 gatgcggcgc tccggccaaa ttcatttttc gcatcgctcc gtctatcttg atgccgggca
      901 gttcgatctt taagccctcc cgtgtttctt gcaatgacat tctaccccca tcgagagcat
      961 ttttcatatc ccgtgcttta ccatttaatc cgcttgcgga aacggtaacg cagatagatc
     1021 gcaaccgcgt tcatcgccaa tgtcatgaac attaggacaa ggccggtcgc agcggcattt
     1081 aaatgaaact cttcctgcgg gcgtgagacc cagttgaaca tctgtatcgg catcaccgta
     1141 aagggagaca tcagccattc gaaagatata aaaggaaatt cagctttgat gggggcacct
     1201 ggaagaaacg caataaacgt caatgcgccg atggtaatga gcggggcagt ctctccgatt
     1261 gctcgagaca aggcgataat gacgccggtc agaatgccac cggtagagta gggaacaaca
     1321 tggtccttga cggtttgcca tttggttgcg ccgagagcta aggcggcctc gcgcatgctc
     1381 ccgggcaccg ccctgacggc ctctcgtgtg gcaacgacga caatcggtaa aatcaacaag
     1441 gccaaggtta atcccgcggt taagatgctt tgtccgaggc caagctggta cacaaacagg
     1501 cccagagcca ttaggccgta tacgatcgac ggcaccccgg cgagattagc gatattgatc
     1561 tcgataatcg cagtaagcca gttcttgggc gcatactctt cgaggtagac gccggcggcc
     1621 acgcctaaag gaacggcggt gaacgcggtc acgatcatga ccaatatcgt gccgacccag
     1681 gcggagagga tccccgcgcg cgaggcaaaa cgcgaaggaa atgaagtgaa gaattgccaa
     1741 tcaagcctgc ctgccccatc tatgccgagg tccaacatga gcgcagccag agtaagcagg
     1801 ccgacgaaag tggccaatag accaaacagg acgaatagac gatcgaccgt ttcgttgcgg
     1861 gcgatacgtg ctttgaggtt catagcttct ctcattagta aatctcccga aatcgcttgc
     1921 gcagcagata gccgccgata ttgaaaatca acgtcatcaa catcaaggtc aatcctgcgg
     1981 cgaagatgct ctgataagcg atactgccat ggggcaggtc gccgaggctc acttggacaa
     2041 tgtaggccgt gatcgttgcc gcgccttcca tgggattgag cgtgaggttc ggctgcatgc
     2101 ccgccgccac agcaactatc atcgtttcac ccacggcacg tgagaagcca agcacatagg
     2161 cggaggcgat gcccgagaac gcggcaggaa aaagcaccct gagcgcggtc tgtaaccgag
     2221 tcgcgcccat cgcgtaagaa ccctcgcgga tgtggaccgg cacgactctc atcgcatctt
     2281 cactgagcga gctaacatag gggatgatca tgatcccgat caccatgccg gcgctaagca
     2341 tattgaaacc gggtagattc ggtaacaccc gctttaaaag cggtgttaca aacagcagag
     2401 cgaaatagcc gaacaccacc gtcggaacgg cgctgagaag ctccaaggtc ggcttgagta
     2461 tttctctcgt ccggctgccg gcatactcgc tgaggtaaat ggcgatgatg gtaccgagtg
     2521 gaagggagac caccagggcg acaaaggatg tcaccaatgt cccggagacc agcggcatga
     2581 tgccataatg cgggtcggca aagagcaccg tccactgagt gtcggtcaaa aattcaagaa
     2641 tcgatacatc cttgaagaat tggagcgatt caaaaaccag aataccgacg attccaacgg
     2701 taatcaggac cgaggaaagc gccgccagga acagaaaaaa ttcaatgact cgttcgcttg
     2761 ctcgtcgcaa accgcggatc atcgcggcgg gtcgaattcg gtttcctaag atcgcaggat
     2821 gcgcctgtcc agtagttgcc atatatgttc agttagctcg gcagcaaagc acccgattta
     2881 actcctgcct tgctgctcct cagttgaaac tcttaattag atttgctttc gcgcttcagc
     2941 aaatcttcga tcttgacgcc tacctcggct tggcctccga acacggtgtc ggttcgacct
     3001 ttttggatat gctccagagc gagagtgtag gctttgactg gcagcgcaac atatttaact
     3061 tcttttacca gcgcggccgc attcttgaga tagaattcga taaactcctt gacctccggt
     3121 ttggcaatcg cctttttgct tacatagatg aaaatcggcc gagacagcgg ttgataggta
     3181 ccgtcctcga ctgtctttgc tgacggagct accggacctt tgccgccgtc gatcgccacc
     3241 gctttaagct tcttttgatt ttctatgtaa taggcgaagc caaaaaagcc caatgcgtta
     3301 cgatcattag ccactccctg aacgaggacg ttgtcatctt cactggccgt aaaatcacca
     3361 cggctcgact tagccttgcc tacaatagct tcggtgaaat aatcaaaggt gcccgagtcg
     3421 gctccggcac cgaaaagctt gagcggcgca tcgggccagt tggggcgaac ctggttccaa
     3481 ttggtcactt tgccttgcgc ggccggctcc cagatttttt tcagttcggc gaccgtcatt
     3541 gacgccgccc agtcgttttt tggattgacg atcaccgtta atgcatcata ggcgaccggt
     3601 agctcgaaaa actccacgcc ggaacctttg caagcttcta tctcttgttt aaggatcggg
     3661 cgggaggcgt cgctaatgtc agtttcccca cggcagaatt ttttgaagcc gccgccggtg
     3721 ccggaaatgc cgacagtgac tttaactttt ccttttttgg atttttgaaa ctcctccgcc
     3781 accgcttcgg tcactggaaa gacagtgctg gaaccgtcga tctgtatgac ctgagccgtt
     3841 gcagcctgcg gcaaagcagc aaggaaagcg gcaaagacaa atgatgtcac tagtttactc
     3901 atcacttttc tccttctttg gtaattatgt aactaatcga tgttacgaaa ttgttacgga
     3961 ccagttacgc ttaggtaaaa ctccacacgg acttcggcaa cgcgacacgg attcctgctg
     4021 cgagatgtct acctatttga cgacaacgtt atggttcaac agtgaagccg gcgtaacaga
     4081 gaacttaaga aagggttaaa tgccgtccaa cgggtaaccg tggacacatt tattccgtag
     4141 cacggcacga gtggaatcgg agaaagccct ttacgcccgg cggtcgcagg ttcgccgcta
     4201 caatgcgggg aactgctctc attgcgagcg tatgtatcgt gcggctaagt tcttctgagg
     4261 ctagcggctt ggatgaccgg ttttagaggg aggacgacac aggttcttgg gcctccggcg
     4321 atgctttgaa atggtctttg aagctgccta gtttgagctg gagcatttcc gcttgtttta
     4381 agatcgggtc gaagcgttca ttattcttgc acgcttcttc ggccacatcg cgcgttagta
     4441 tctgttgaag ctccgggtga tcacgaacaa attctggctt ctcactcaga tctgtgacga
     4501 agtgcaatag cacatgccag tcgtgccaac gaccgagctc atcctgcaag gctttcaacg
     4561 agtttacata ttctgcggtg ccattgtcga gttccgcgag aacttccaaa cgatagcgga
     4621 gccgtttgcc tgagatccgc atttcgtgaa gatgcttcga atcaggattg ctttttgcct
     4681 gcacgatcgc ttcgtcccaa tccgtcagag tgtcagcaaa aatctcccgc gaatctaggt
     4741 tttgaagctt ctctgttttt acagtgtcga acaactgctc agttcttcgt ataaaagaga
     4801 gaatatccag attgcgaagt cgcttctttg cacggtcgaa ttcctgagct cgcaactcca
     4861 acaagcatcg ccgaacataa tcccaagcat cgcgagctcc atccgcagta atcgactcga
     4921 gcttctcctc cagcagaact agattaacgt caaaattacg gcaagcgctc caatcgcggc
     4981 gcagtttacg tagagatcgg attactttac ggctttttcc atggcgcggt ttgggaaaca
     5041 gtaggctaat aacttgctgc aaccgccgac ttgagactcg aagatcgtga atgtcctttg
     5101 aatcatcgtc cactaaaagc cttaccgcga tcgacactcc ctttttcaag cgagttcgag
     5161 acaatttcag gatatccgtt ttcgaaacag tgagaagagt gtctggcttc tcagagttgc
     5221 tcgcgtctag acccatttgt agcagattac cgagccatca ggcggagctg tttcggagtt
     5281 agaagccagt gcatctgccc aggttctgtc ggcggaacag cgttgatctc gatgcggcag
     5341 ataccgccct tttttaggag aatcgaattg tcagaattgc tttttccgtt aagtaaaaaa
     5401 aacgccaatt tgccaaggaa cggctcatgg cccaccaaca tgacatgctc tttgtcgtta
     5461 atgtcgtgca aggctgatgt gagctgctcg ggcagactct ctggagctaa ttccgcaatt
     5521 tcttcaacgc aagattcgtt gttaagaacc ttggctacga tatcagctgt ttggcgggcg
     5581 cgaacgagag gactgcttag gatcatgtca aacgtaatcg ccaaacgctc aattcctcgc
     5641 gccgctttgc ggaatttttt aattcctttg ttggtaagcg gtcgctcgct gtccgacgca
     5701 aaagcgggat cacccttggt tgcagcgacg gcgtgtcgta gaagataaag attcatagcg
     5761 tcaccttact tgtcgatact caattgatga cggcggaagc actggggctt cgcatcattc
     5821 cgccaatata atgagaatcg cgaataccgt ttgctgatct agtctgctca tattctccgg
     5881 caacgggtcg ccccttttcg ctcccctttg aacttatcat caaatcgatt tcattcaacg
     5941 gtgtatcatt catcttccac tcgaccgtcc attccgtctc aataccgatt cccctaattt
     6001 gtaagttcga gttaggatag ttgaggtctg acgttagagc tgaaggtcgt aactttgaga
     6061 gtgtcgaact gttagcgact gctccggccg tttcggatca aggtcggatt ttccagcatc
     6121 gaatattccg gtcgcgaata gttcgtctca tcgagttcac cttgggttat gactctatgg
     6181 tctatgcctt ccggataatg agcctatggt tgaaaacttc ctcaaagagt tcgcccttgt
     6241 acaaggcccg agcaacttcg tcgccgacat tccccgtccc ggaagctgtg atagtgatag
     6301 ccttggattt cttcgcacat gtcagcgcag taaccttttg gtcgtgccta taatcgaacg
     6361 cgtcggcaat tcgaagtaga gcgctcaatt tccgcaccac atcctgattg gcaggtgaaa
     6421 gttgactgaa accgagatgc ttctggcttg ggtgcgattt acgatgataa cgggcgatgt
     6481 tggcgaccac atcgagattg agtttatcga aggcgttcat ttcggaactt ttaattagat
     6541 agtaggaatg tttatggtgc ttgggctcgg ctatgaaaag cccgatatcg tgcagcatcg
     6601 ccgccgcctg aagaaggctg gcgtatttgt ccgtgagctt atgtaaattg ttcagttgat
     6661 aaaatagatt cagcgcaagc tggctgacgt gataggcatg aggcgcatcg taaaggtatt
     6721 tttgacatac ctgttcaaga gattccgcac gctccgcatc cggatcccac acacctgact
     6781 tatcatagtg tcgctgcagg agatcgatca tcaatccatc ccgcaaccct cgtcgcgaga
     6841 caaatatata atcgaggccg atctcagaca tgatctcata gagaaccagg ccgccggcaa
     6901 caataatgtc ggcgcgcttg ggatcgccgc tgatcagcga ggctcgctgt ttaaccggta
     6961 tggtcgttag caagtccaat aaggctttca atcgcgtgcg ccgcaagatt gccagagatc
     7021 cgtttttacc atcgccagct actcgtaaat tggtttcggc caaagaagtc atggtaccgc
     7081 cgctgccgaa agccatggta aacttttcct gcccaacgcg ctgacctacc gaccggagct
     7141 gctgtctgac gaactgacgt aaagctcgga tttcacgttt tcgcggcgga tcagcagtga
     7201 ataactcttc ggtcagacga accgcaccga gaggtaggct gaacaggcaa aaaatattgg
     7261 ttgtattggc gacggcaagt tcaatgctgc cgccgcctat atctaaaaac agcccaagcg
     7321 gcagataaaa acgcaggccg ctcataatcc ctagacttat cagtcgagct tcgtcctcct
     7381 ctgagagaat ctgcatcggt acgccgagct gatgcgcctc tttgagaaaa gattttccat
     7441 cgcaagcttc tcgtaaagcg ctggttccta ccgcttggat tgccttgacc tgatatcgtt
     7501 cggcgactga tttaaaatcg gcaagcgcct tcaaagctgc ttcagcagta gcgggatcca
     7561 ggtgaccggt tctaaacaca ttgtgcccga ggcggatagg ttggcggtcc tgttcgagca
     7621 cgcgatatcg tttgggctga tcgacaagag cgacctgaaa tttcaacgcg ttgcttccaa
     7681 cgtcgatgac tgcaaaatag tcggccatct tcattccata gcactgattc gaccgtttgt
     7741 tgatctaaaa cacgttacaa atcggttaaa tattttgccg gcggctacac caacccgctt
     7801 gttcgtaaca atgatttaat ggtttattag cagtcgaggg gttttcgtcc ggtaaacggt
     7861 attataggtt ggcctaccta tgctgattct cagacgaacg accgttgaat ctatcgagga
     7921 tttgccgaca ggacagaaat atggacggag acaagaacca taggtatctg cagcgggagt
     7981 tgtcatggct tgaattcaat cgacgtgtcc tcgaggaagc caaagatgag actgtgccgc
     8041 tgctagaacg ggtaaagttc cttgccatct ttagttccaa tctcgacgaa ttcttcatgg
     8101 ttcgtgtcgc ccgcatcaaa cggcagcttt tcaaaggagc tcagcatccg acccccgatg
     8161 gacttacacc ggctgaaatc ttaacagcta tctcgaaacg agctcacgag cttgttgagg
     8221 aacagcatct gtgtttcatc gacataatct tgccgcggct agccacagag ggaattcaat
     8281 tggttcggcc ccaagagatg agcggtgagc aggagcgttt tctcgaagac tttttcaatc
     8341 gaactctcta tccgattcta accccgcttg ccatcgatcc gggacatcca tttccatacc
     8401 ttgccaaccg ctcgttatgt tttgtcgtgt cgctgagtcc caccatcgct tcacgtttgc
     8461 cgcacaccga tctttcaatt cttcatatac caacgcatgt cgtctctcgt ttcatctcgc
     8521 tgccgacccg ggaggggcag cacgcgtttg ttcttttgga agatgttctt agccagtacg
     8581 tgccgcgcct ttatcacggt ttcgagatcc gatcgtgtca cgccatccga gtgactcggg
     8641 atgccgaatt tgggatggca cgccggcacg atgacgatct cttgatgacg atcgagcagg
     8701 gtgtccggga gcggcgtatg ggtgacgcgg ttagattgca gtatgatccg gagctgccgc
     8761 aagactttgt tagccaatta gtcgatgaac tcgagttagg agctgaggat ctgtatccga
     8821 gcacgggctt tactgcgttc actgatctct tccagcttta ttccgccgtg aatatgcctc
     8881 gcctaaaaga ccggccgcag acagctttac cggtggcggc tttcgaacgg gcgggggata
     8941 tctggagcgc tatacgtgcc ggcgacatta tgctgtttca cccgtaccat tcttttgacg
     9001 cggtgactcg ttttgttgat gaagcggcaa aagatcctaa agtgctggcg atcaaaatga
     9061 ctctttatcg ggtttcccct gcctcgccca ttgctcaggc cttggcgcgt gcagctgaat
     9121 gcggcaaaga ggtgtcggtg cttgtcgagt tgcaggcaag atttgatgag gaagcaaata
     9181 ttacttgggc acgggctttg gaagaagttg gagcgcacgt ggtgtatggg ttggtgggat
     9241 acaagacgca ttgcaagata tgtctggtgg tgcgtcagga aattgacggg atccggcgat
     9301 actgtcatct ggcgaccggc aattacaacg tgcgcacggc aggtgtttat agcgatttag
     9361 ggctatttac ttgccgtgaa gcctttggtc aggatctcac ggaattattt aatctgctga
     9421 ccggctacac cagaccgcag aggttccagc agttactact cgcaccaacc gggctgaggg
     9481 aacacttcat caactgcatc cgtaaggaaa aggagcatgc gcacgccggc gaatccgcgc
     9541 gcatcatagc caaagtgaat ggcctaacag atccggcgat catcgatgag ctttatagcg
     9601 cgagccaggc aggggttcag atcgacttga tcgttcgcgg catgtgcagt ttgagtccgg
     9661 gcgttgcggg agtgtcggag aacattcgcg tcatctcgat tatcgatcgc tacttagagc
     9721 acgcccgcat tttctatttc tataatcgcg gcgagtccac gcactggtta gcttccgccg
     9781 attggatgcc gcgcaatttc gatcggcgcg ttgaaatagc ttttcccata ctcgatctcc
     9841 aacatcagct tagattaaaa gagatcttgg agattcagct cagcgatact gccaaggctt
     9901 ggcagaccca acctgacggt agttcattac ggatcgccaa tgcggatcga ccatttatcc
     9961 gatctcagga ccggctctat gaaatcttac gagccgaaaa cgattcgaaa aaggaggtga
    10021 tctagcacag agttttgttc agtcatcccg ttagtcttcg gccattggat aacctatccg
    10081 tccacgacat ttcaagcaca gatcgctcgt acagttacgc gttaatgatc gcctcgcaag
    10141 acgctcagac agatgtctga tcgtactcgt caataaccgg tttagcccgt gcatggcggc
    10201 gtctactgga aatcaagcga aacagccgag cacggggtgg agaatcattg gcctcgcgag
    10261 agttgatcaa tcaaacgcgg ctgctcccgt ttctttgcag cagagacagt tggcgtatcg
    10321 ccatcttgat aactgtgtta ctcaagaaac atgtactgaa cacgtcgtga atatcggtcc
    10381 ccaatgcaac ctccagataa aggccaattt aaattccgat gaacactgac cgggagaagt
    10441 gtagatctcc gagcagccgg cttgtaaaac gaattacgga ttagaagaaa tcctaccaac
    10501 catgtgaacg tttctgctta gagctttaac gaggacggaa tgatggaaat agatacaatg
    10561 atagagagcg ccagagagtt gatcaaaccc tttcgcgtca ccgatcgtca gagttttcgt
    10621 ctcaaagatt ttgatcctgc cgacacactc cacttaaact ccgaggataa accgcgcgcc
    10681 aaggaaggcc ttgccgccgg aatcgaggtc ttggcgaagc tccaagataa actttacgca
    10741 cagaaccgtt gggcggtgct ggttatcttt caggcgatgg atacggcagg caaggacagc
    10801 gtcattaaac atgtcatgtc cggcgtcaat ccgcagggtt gtcaggtcca ctccttcaaa
    10861 gcgccaagcg gcgaggagct cgatcacgat tacctgtggc gctgcgcgag acgtttgccg
    10921 gagcggggta tgatcgggct attcaatcgc agctactacg aagaaaccct ggttgtgcgg
    10981 gtccatccgg agttgttgga gaagcagaag ctcccgcctg aacttgttac caagaatatc
    11041 tggaaagagc gattccagga tattcggaag tttgagcgct atctaacgcg caatggtgta
    11101 gttattcgca aattttatct gcatctatct aagaaagagc aaaaaaaacg gttcatagag
    11161 cgaatcgata aacccgagaa gaactggaag ttttcagtga atgacgtcca tgaacgcgaa
    11221 cactgggatg gctacatgga agcatacgag gatatgattc gccacacctc gaccaaacaa
    11281 gcaccctggt acatcgtgcc ggcggataac aaatggttta ctcgcctcat cgttgcggct
    11341 gctctggtgg accttctcgt atcgctcgat ttgaattatc caaaactaag caaagaggaa
    11401 ctcaagcagc tcgacgaagc aaaagaaagg ttattgaccg ttaagtagtc tccgtcttat
    11461 tagaagggga aaaggctcgc cgtttccgaa cgtcgggcta tcttctcatc ggcatgctgc
    11521 gcattctgtt tggtctcgac gaaatgctcg aacatctttc gttccattgg agaatagacg
    11581 atagccagag tccgcaacac gcctagtacg agcgctagga acgcgagcgc cataatcaac
    11641 agtggcgggc tttgatgttg gtagagactg atcatgacct cgcgcagtat gaacaccaag
    11701 gtagcatcga caataaacgt cagtctaaga cggtgctcgc gaaaatattc cactaggctt
    11761 ttaaacagct ccaggattat gaaaaatgtc agcaggttgg tgaccgtaaa actgaaagcg
    11821 tctttcagtt ggctgactgc catgatcctc caggtctccc aaaacagcct gacgagtccc
    11881 atgccgagaa caacgacaac gaagaagatc aatagcttga aaaacagatc catgacccgg
    11941 ttgaagaacg tcaacgcatc gaagtgttgc catagtcgca ataactcttc ctcccgagcg
    12001 agacgacgag actgccttat cgtccgcccc gttatgatag ttgagcaagc tctcctccgt
    12061 cgtggtcaaa tactggcgaa ccttggt
//
LOCUS       DASUVS010000329         6925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_357205, whole genome shotgun sequence.
ACCESSION   DASUVS010000329 DASUVS010000000
VERSION     DASUVS010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357205"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VF182_17610"
     CDS             complement(<1..388)
                     /locus_tag="VF182_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438956.1"
                     /translation="MFTDVRRLIVPVILSFTVHVSLAYAETHDFYKGKTIRIVVGFTA
                     GGAFDVYSRTIARYMGQHIPGNPTIIVDNMTGAGSLVAANHIYKAANPDGLSIGNFIG
                     GLFVGQVLGRPGIEFDALKFEYLGSPM"
     gene            complement(428..868)
                     /locus_tag="VF182_17615"
     CDS             complement(428..868)
                     /locus_tag="VF182_17615"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6438957.1"
                     /translation="MIARRYAGRPGERSRQDDAGREYLKGRVETERSYSIAVSAFGKR
                     TIYVSGIAAWEDETGKSLAGDFNGQTRVVFGKLRQILETLGATLDDIVSMSVFVTDMQ
                     YGQTFTKIRKEYFIRDYPASALIGIKELARPPMMIEIQATAVTD"
     gene            1073..2779
                     /locus_tag="VF182_17620"
     CDS             1073..2779
                     /locus_tag="VF182_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011427907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6438958.1"
                     /translation="MNIRRTRIVTGLVLFTYLSTHLLNHSLGLISLDAMEAGRVWFLA
                     LWRNPLGSTVFYGSLLTHFILALYALYQRRRLRMPFYEALQLILGLCIPLILATHFVG
                     TRMAYEWYGIEDMYAKTVLSLWRQNPINGLRQASLVLIAWTHGCIGLYFWLRLRPWYP
                     RYAAALYALALLLPVFALLGFVEAGREIDHLIARNPEWIEQLYQRTNFFRPGQGEDLV
                     NLRDGIIYGFWASLAAVLVARAARHIFERPGRVRITYPDGSQVHVPRGFTVLEASRSA
                     RIPHASVCGGRGRCSTCRIRIVKGLEGLSLPSPAELKVLKRIGAPANVRLACQLQPTR
                     DIAVVPLLPSDANASAGFVKPSHLAGKECDMTVLFADLRSFTTIAETKLPYDVVFILN
                     RYAEIVGTAIERAGGIPNQFTGDGLMALFGVETGPEQGCQSALAAAREIVSSIAQLSE
                     MLSEELETALKVGIGIHTGPSVVGQMGYGMGTYLTAVGDTVNVASRLQELTKEYDCAL
                     IISEQVTQRAGIAMQSSPRHQVSVRNRREPLIVYAIGDESRLLSPSISPNSRVNSSEV
                     TS"
     gene            3078..4058
                     /locus_tag="VF182_17625"
     CDS             3078..4058
                     /locus_tag="VF182_17625"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438959.1"
                     /translation="MLKQLLTLTAVSLLCSQPSAAQEKRDLRVAYVSPSYSQSINWIA
                     KETGIFSKHGLNVELIYMSGGKSTQALVAGSIDFSSNAGPPLVQAGLAGADPVLIISS
                     ITVPIFSVIVRPEITRPEDLRGKTIGSSGLNNLSYFITRRVLKKWQLEPDRDVKIIGA
                     ANRLATMQQGLIQGTLLSPPENFAAQKLGFKSLGEVAEMGINYSYAGIISSKRFVNEN
                     PDSTLRFTRSYIEALALLLRDRSTAVRIVEKYSRISDRAVLENAYDLFAKHWRATPLP
                     DREGVRGILEDLAAQEPKAKTAEPDNFIDPTFVRKVSQEGLIDKLFGAKK"
     gene            4246..5238
                     /locus_tag="VF182_17630"
     CDS             4246..5238
                     /locus_tag="VF182_17630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6438960.1"
                     /translation="MKFSLHSVHGGVKTIDEIHKRARRAEELGYDGIFLGESHLSTID
                     SFQTLASCAMITERVLLGIAVTNIVFRHPTVIAGAAASLNEISKGRAIMGLGTGDGPV
                     YTQGLKATPMKDFEHGVKLIRNLVRGKQADFSTGKVGIGFTIHQPAPIYVSAEGPKGL
                     QLAGRSADGVILGTGFDLKVYEWAKQKIADGTCEAGRDPHEIKIIAAGMLCVDEDGNK
                     ARKVVRNRIANRAHHNFRFTLETVPAQELPAIKKFMDGFDVMKPMEERVDPDLVNDYL
                     VHRFSIAGNPKECIERIEELQAAGVEHLMLTPARRVYSETVEAFAKGVIPHFKK"
     gene            complement(5408..6007)
                     /locus_tag="VF182_17635"
     CDS             complement(5408..6007)
                     /locus_tag="VF182_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HEX6438961.1"
                     /translation="MQITDILAQMGGLESMARELGVSENQAASGAEALIPAILGGFKK
                     QAQTQPSGVEGLGDMFGQLGGGALMDDVLSPEPTHVDRGDDVLGQIFGSKDVSRTVAQ
                     NAASQSGLEPSLLKKMLPMLAMLVAGYMAKQGSGANPAQASPGGGLGDVLGGLLGGQA
                     SGAGRTTSGTGGGLSSMLDLNGDGNPLDDILSMAGKVLR"
     gene            complement(6045..6437)
                     /locus_tag="VF182_17640"
     CDS             complement(6045..6437)
                     /locus_tag="VF182_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HEX6438962.1"
                     /translation="MAFFGKILEKLGFGSSAAAAPQSSTAAPSGSGGAATQAAPKPVS
                     TVDVVAQLEQRAAANPQKLNWRTSIVDLLKLLDIDSSFNARKELATELGCPADLMGDS
                     AKMNMWLHKTVLQRIADNGGNVPKELLD"
     gene            complement(6739..>6925)
                     /locus_tag="VF182_17645"
     CDS             complement(6739..>6925)
                     /locus_tag="VF182_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6438963.1"
                     /translation="ALLSEKTQSCLLRGRGNVVVGKTLQQVVYRTIQTQLNARLQLQA
                     TMLVGPLIYMRRRKPRR"
BASE COUNT         1480 a         1984 c         1912 g         1549 t
ORIGIN      
        1 tcatcggcga accaagatat tcaaacttaa gagcatcgaa ctcgatccct ggccggccaa
       61 gtacttggcc gacaaagagt ccaccgatga aattcccgat ggagagtcca tcgggattcg
      121 cggccttgta gatgtgattg gcggcaacca ggctgccagc tccggtcata ttatccacaa
      181 tgattgtagg atttcccgga atatgttgcc ccatgtacct agcgattgtg cgggaataca
      241 catcgaaagc cccgcccgcg gtaaatccta cgacgattct gatagtcttt cccttgtaga
      301 agtcatgagt ctccgcatat gcaagactga catgcaccgt aaaactgaga ataaccggta
      361 cgatcagcct ccgaacgtcg gtaaacatct aacccctcct aagcttttaa cgttgtcggg
      421 gacgccttca atcagtcact gcagtagcct gaatctcgat catcatcgga ggacgcgcga
      481 gctcctttat accgatcaga gcgcttgcgg gatagtccct gataaaatac tccttccgaa
      541 tcttagtgaa cgtctgccca tactgcatgt cggtgacaaa aacagacatg gagacgatat
      601 catccagcgt ggcgccgagt gtttccaaga tttgacgtaa tttaccaaac acaactctcg
      661 tctgtccgtt gaaatcacca gcaagcgact ttcctgtctc atcctcccaa gcggctatgc
      721 cggatacata gatggttctt tttccgaaag cactgaccgc tatcgaatag ctgcgttccg
      781 tctcaactct gccttttaaa tattcgcgac cggcgtcgtc ttgccggctg cgctcgcctg
      841 gccgccccgc ataccgccgg gcaatcaccg acgccgccgc ccgtaggacg cctgcgcgtc
      901 ggactgccgc cgcttcgctg ctttgccatg tagcctgcaa cgagcttggc gagcatcggc
      961 agcatctttt tgagcaacgc gccgcgctac tacaccgcat cgccgatgct aaatcagccg
     1021 cgctgcggcg cgtagacggc atgcttgcct cgtagtagat tggcgcaact ttatgaacat
     1081 tcggcgtacg cggatcgtca cgggcctggt gctgtttact tatctatcca cccatctgct
     1141 caatcattct cttggattaa tttcgctcga cgccatggaa gcgggccgtg tatggtttct
     1201 ggctctgtgg cgcaatccgc tgggtagcac ggtcttctac ggctcgctcc tgacccattt
     1261 cattttggcg ctttacgcgc tttatcaacg aaggcgcctg cgcatgccgt tttatgaggc
     1321 gctccagctc atactcggtt tgtgcatacc gctgatactg gcaactcatt tcgtcggcac
     1381 ccgcatggct tacgaatggt acggcatcga agacatgtac gccaagaccg tgttgagctt
     1441 gtggcgtcaa aacccgatca atggtctgcg tcaggcttcg ttggtgttga ttgcgtggac
     1501 gcatggatgc atcggcctgt acttttggct cagactaagg ccatggtacc cccgctacgc
     1561 cgcggcgctt tacgcgcttg cgctgctgct gccggtgttc gcattacttg gtttcgtcga
     1621 agccggccgc gaaatcgatc acctgatcgc gcgtaatccg gagtggatcg agcagctcta
     1681 tcagcgaacc aactttttcc gccccggcca gggcgaggac ctcgtcaatc tgcgggacgg
     1741 aattatctat ggattttggg catctctggc tgcggtgttg gtggcgcgcg cggcgcgcca
     1801 tatctttgaa cgcccgggca gggttcgaat cacctatccc gacggaagtc aggtgcacgt
     1861 gccgcgcggg tttaccgtgc tggaagcgag ccgttctgcg cgcattcctc atgcgtctgt
     1921 ctgcggcgga cggggtcgct gttctacctg tagaattcgc atcgtcaaag gattggaagg
     1981 tctgtcatta ccaagcccgg cggaactgaa agtcctcaag cgcatcggcg cgcccgccaa
     2041 cgtgcggctg gcgtgccagc ttcagccgac gcgcgatatt gccgtcgtgc ctctgcttcc
     2101 ctcggacgca aatgcaagcg ctggctttgt caagccttcc catctggcag ggaaagaatg
     2161 cgacatgacc gtgctgttcg ctgatcttcg ttctttcacg accattgcag aaaccaaact
     2221 gccctacgac gtggttttca tcttaaaccg ctatgccgaa atcgttggaa ccgctatcga
     2281 acgcgcggga gggattccga accaattcac gggcgatggc ctcatggcat tgttcggggt
     2341 tgagacgggg ccggagcagg gatgtcagtc tgcccttgcg gcagcgcgtg agattgtcag
     2401 cagtatcgcg cagctcagcg aaatgctctc tgaagaattg gaaacggctc ttaaggtagg
     2461 catcggcatt cacacgggac cttcagtggt gggacagatg ggctatggta tgggaacata
     2521 tcttactgcc gtcggcgata cggtgaacgt cgcaagcaga ttgcaggagc tcacgaaaga
     2581 atacgattgc gcgctgatca tttcggagca ggtcacccag cgggccggaa ttgcgatgca
     2641 gagttcaccg cgccatcagg tcagcgttag aaatcgccgg gaaccgctga ttgtgtacgc
     2701 aatcggtgat gagagccgcc tgttatcgcc gagcatctcg ccaaattcgc gagtcaacag
     2761 ctctgaagtt acaagctaag acatcctgcc gttcgcgccg gctcatcgcg tcaagataac
     2821 ccgctcattg attggagttt ccttttgcgg cgaccgtact cagtcgagcg atagaaagtt
     2881 aacgcagtaa actcagtttc cgttcgagaa ccgtgcacgg ctgtcagaca ctttgagcca
     2941 gcaagtacga cgttgggaag ggatcgttcg cctggcgatt gcaacccgtg tcttacgcgg
     3001 ttcacttggt tgacagccgc tgaaatgcaa tttatagaca acaagttttc gaaaaattaa
     3061 cgaaacgagc cgtcgacttg cttaaacaac tgctaacttt gactgctgtc tcattgttgt
     3121 gctcgcagcc gagcgcggcg caagaaaaac gcgatctacg cgtcgcctat gtgtcgccga
     3181 gctacagcca atccatcaat tggatcgcca aggaaaccgg catcttcagc aagcacggct
     3241 taaatgttga attgatctac atgtcggggg gcaagtcgac ccaagcgctc gtggcgggca
     3301 gcattgattt ttcatcgaac gccggaccgc cgctggtgca agcgggtttg gctggcgccg
     3361 atccggtttt gattatcagc tcgatcactg tgccgatctt ctcggtcatc gtcaggcctg
     3421 agatcaccag acctgaggat ctgcgcggca aaacgatcgg ctcttccggt ttgaacaacc
     3481 tttcctactt catcacccgg agggttctca agaaatggca gctggaaccc gatcgcgatg
     3541 tgaaaattat cggcgccgcg aatcgtctgg cgacgatgca gcagggattg attcagggca
     3601 cgctgctttc gccgcccgag aattttgccg cgcagaaact gggatttaag tctctcggag
     3661 aggtagcgga aatggggatc aactactcct atgcgggtat tatctccagc aaaagattcg
     3721 ttaacgagaa ccctgacagc acgctgcgct ttacgcgttc ttacatagaa gcactggccc
     3781 tgttgttgcg ggatagatca acggcagtgc gcattgtcga gaagtacagc cgcatctccg
     3841 accgcgcggt gctcgagaat gcttacgatc tgtttgccaa acattggcgc gccacaccac
     3901 tgcccgaccg tgaaggcgtg cgcggcattc tggaagatct cgccgcacag gaaccaaagg
     3961 caaaaaccgc cgagccggac aatttcatcg atccgacatt cgtgcgcaag gtctcgcaag
     4021 agggactgat cgataaactc ttcggcgcga agaagtaagg ccggccgcga ccgagtgcgt
     4081 gctgattgcc ctgctcggcg agcaaaacta gcggcgtcac cggcaaaaac agggcaccgc
     4141 tttggcacga agagcagcgg agacaccagc ttggaatttc gttgctgatc ctttcgtgat
     4201 cgtcgttcca ttctttatgc gattaatgcc tggaggtatt tttcaatgaa gttcagcctg
     4261 cacagcgttc atggcggcgt aaaaaccatt gacgagattc acaaacgcgc gcggcgcgcc
     4321 gaggaactcg gctacgacgg gatttttctg ggcgaaagtc atctgagcac catcgattcg
     4381 ttccaaaccc tggccagctg cgcgatgatt actgagcgag tgcttctggg catcgccgtt
     4441 accaacatcg ttttccgtca tccgaccgtc atcgccggcg cagcggcatc gctgaatgag
     4501 atctccaaag gccgcgccat catgggactc ggtacgggag atggtccggt ctacacgcaa
     4561 ggactgaaag ccacgccgat gaaggacttt gagcacggtg tcaagctgat tcgcaatctt
     4621 gttcgtggta aacaagcaga tttttccacc ggcaaagtcg gaatcggttt cacgatccat
     4681 caaccggcgc cgatctatgt ttccgcggag ggcccgaaag gcttacaact cgccggacgc
     4741 agtgccgacg gcgtcatcct gggaacggga ttcgacctga aggtttacga gtgggcaaaa
     4801 caaaaaatcg ccgacgggac atgtgaagca ggtcgcgatc cgcacgaaat caagatcatc
     4861 gctgccggta tgctctgcgt tgacgaggat ggaaacaaag ccagaaaggt cgttcgaaac
     4921 cgcattgcca accgcgcgca ccataacttc cgcttcactc tcgaaaccgt tccggcgcaa
     4981 gaactgccgg cgatcaagaa attcatggac ggcttcgatg ttatgaaacc aatggaagag
     5041 cgcgttgatc ccgatctggt taacgattac ctggtccacc ggttttcgat agccggtaat
     5101 cccaaagaat gcatcgagcg catcgaggag ctacaagccg ccggcgtcga acatttgatg
     5161 ttgacgccag cgcgcagagt ctatagcgaa acagtggaag cgttcgccaa aggtgtaatt
     5221 ccgcacttta aaaagtagct ttgtctcacc cgacatcgtc gtagaatgac tgtgacggcc
     5281 gccacgtccg cgacttcaac gccgtccagc tgcttttctt cgggccgcaa ggcgccgccg
     5341 tcttgttctt taggcaagtc ccgacaccag tggtctgaac caaaaagttt agttgatgat
     5401 gaagcggcta acgcaacacc ttgcccgcca tgctcaagat gtcgtcgagc ggattgccgt
     5461 cgccgttcag gtcgagcatc gaagagagcc cgcctccggt gcctgacgtc gtcctgccag
     5521 cgccgctcgc ctgaccgccg agcaaaccgc cgaggacgtc accgagtccg ccgccgggcg
     5581 aagcctgagc cggattggcc ccgcttccct gctttgccat gtagccggcg acgagcatgg
     5641 caagcatcgg cagcatcttt ttgagtagcg aaggctcaag accggattgc gacgccgcat
     5701 tctgcgcgac cgtgcggctc acatccttgg agccgaagat ctgtccaagg acgtcatcgc
     5761 cgcgatcgac gtgcgtcggt tccggcgaaa gcacgtcgtc catcagcgcg ccgccgccga
     5821 gttgtccgaa catgtcgccg agaccttcga ccccggatgg ctgtgtttgc gcctgctttt
     5881 tgaaaccgcc gaggattgcg ggtatgagcg cctccgcgcc gctcgccgcc tggttctcgc
     5941 ttacgcccag ctcccgggcc atggattcga ggccgcccat ttgggcgagg atgtcggtga
     6001 tctgcatggg tttgactcct tgattgattg ttttgagcgg ctcttcagtc gagcaattcc
     6061 ttgggcacgt tgccgccgtt gtcagcgatg cgctgcagca ctgtcttatg tagccacata
     6121 ttcatttttg ccgagtcgcc catcagatcc gccgggcagc ccagctccgt ggccagctcc
     6181 ttgcgggcgt tgaagctgct gtcgatgtcg agaagcttca gcaggtcgac gatcgaagtc
     6241 cgccagttga gcttctgtgg attggcggcc gcgcgttgct cgagttgcgc gaccacatcg
     6301 accgtcgaga ccggcttggg cgcggcttgc gttgcagccc caccggaacc tgacggtgcg
     6361 gccgtgcttg actgaggggc cgcggcggcc gagctgccga accccaattt ctcgagtatc
     6421 ttaccgaaga atgccatagg gagctctcct ttctgtcggt tgcggagtta atggagcgaa
     6481 ctcaaattcg accttcgtcg tatcgcaata catagtatgc atatcactgg caaacgaagt
     6541 aagacaaggc cacttcccga gaagatcgga ttgtccgatc cgagcaaaaa ttaaacattc
     6601 gggacggatt tctagatctc gctaatcttc ctccagtgtg ttcgcagctc atccgggatg
     6661 atcattgtcg ccggcttcgt tccttccaaa gctcccaagt acgacctatc tgaatggcgt
     6721 cggctcccga gcggtcgttc accttcgcgg cttcctccgg cgcatgtaga tcaaaggccc
     6781 gactagcatc gtcgcctgaa gctgcagccg ggcgttgagc tgtgtttgaa tcgttcgata
     6841 aacaacctgc tgcagagttt tcccaaccac tacgttgccg cggccgcgca agaggcacga
     6901 ctgcgttttc tccgagagca gcgct
//
LOCUS       DASUVS010000330         7970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1450343, whole genome shotgun sequence.
ACCESSION   DASUVS010000330 DASUVS010000000
VERSION     DASUVS010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7970
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450343"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1060)
                     /locus_tag="VF182_17650"
     CDS             complement(158..1060)
                     /locus_tag="VF182_17650"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6438964.1"
                     /translation="MAASNFSLRFWGVRGSYAVSNRGVLKYGGHTSCVEVVAGKRQFI
                     FDAGTGIIELGKDLIKNNQGPLSLNLFLSHTHHDHLSGFYFFDPLFDKRNRINIFGPG
                     SAKRSLAETLRVAMEPALFPIGLDTLDAKKSIWSLVGGERIELRDNRQAPRITQTVSL
                     RDNNSQQPFILTHGSPAHPNGVILYRVFFQGRSVVYATDLEQTPGGYPEIIEFARDAD
                     VLIHDAQYLQSEYTSAANPRRGWGHSTVESATEVAKKARVKRLVLFHHEPTHDDRCID
                     RILKLARQRFPATAVAAEGMVISV"
     gene            1420..2358
                     /gene="phnE"
                     /locus_tag="VF182_17655"
     CDS             1420..2358
                     /gene="phnE"
                     /locus_tag="VF182_17655"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEX6438965.1"
                     /translation="MAQRLQATDSPDIFLEAPPAPRHKLRYFIFTGLFLIILSWAFQG
                     AKIRVGELLEGIPQIAQTLGRMLPPDFSKLTDASYYYFPDELSWRELLLPVPLPEALA
                     RTRQRWWNNIFPQTVFGATLETVQMALAGTFMALIVAFPLGFLAARNTSPHPVVYHSV
                     RTVLNLLRTIPDLALGLLFVAAVGLGAFAGTLALAIHTATVLGKLLSESVENIDEGIV
                     EAIRATGAGYTQILSFAVLPQILPDLISFTLYRLETNIRAASVLGLIGAGGIGYLMNT
                     SFRTFQYQEAAAIVLVLIALVMLVDYLSSRLRTLVV"
     gene            2381..4630
                     /locus_tag="VF182_17660"
     CDS             2381..4630
                     /locus_tag="VF182_17660"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6438966.1"
                     /translation="MDLKVVGRPSRRVDGPDKVSGNTVYTADVQLPGMLWGRCLRSPY
                     AHARILSINTEPAKRVKGVRAVLTGEDLPDKRVGLSLQDTPLLAIGKVRFIGEKVVAV
                     AAEDRDAAEEALSAIEIEYQELPGVFDAGQAMTRDAPLVHEDLATYAGFKTPDVEIPN
                     VFAMQKWTAGDISTGFDEADLVLEHTFRTAVAHQGYIEPHSAIVAVDSSDRVDIWASC
                     KAPFRVKSHLSRQLDIPKERIRVHVVAVGGDFGGKGALMDIPICYELAKAARGPVKMI
                     MTYAEELIAGDPRHSSVIKIKSGVKKDGTLTARETEVIFNSGAYASFKPGDPPNLGGA
                     TFTNGVYHIPNYCIRSYGVYTNSVPCGYYRAPGQPQAVFAAECHMDLIAQELRMDPLE
                     LRLKNLLRNGEKLAGGRAVDKVRVRETLQAAAQKAGWGKKNGNHTGLGIGVSFRHVGG
                     SGKASAELSLSADGKLKILTAVPDIGTGTHTLLRQVAAEVLTIQPELISTEIGDTESF
                     ESDAAPGGSKITNMSGHVVLQAAEQLRQRLTPFAASLLGCAVTDVVLHNGRFGSALDK
                     KRSIEFSAVAREATKSNGDLRVRETFEVKGRSPVPAFVVQIAEVQVDFETGQLRVKRI
                     VTAHDVGTVINPVAHQGQIEGGLVQGLGYATLEEVMTDNGKVLTTNLGDYRIPCARDL
                     PALDTVLIEDPNGPGPFAAKQIGENGIIATAPAIANAVAAAAGVRLFDIPLTAEKIYR
                     ALKSSSPAA"
     gene            4699..5586
                     /locus_tag="VF182_17665"
     CDS             4699..5586
                     /locus_tag="VF182_17665"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX6438967.1"
                     /translation="MGKKFIWGEATSMIRAVGLVAKYHEPKATEMARWLVPWLKKRGL
                     KVYVENGISRSGGISSTKKDMAIKADLIISLGGDGTLLNIAPLVERPDVPILGVNLGG
                     LGFITEVAADELESVLTHTLEGEYQTEKRMTLEVQVRSKNGKAHRFRVLNDAVITKGA
                     RSRIINLETYVDGEYLCNYRADGLIISTPTGSTAYSLAAGGPILAPALSAIILTPLCP
                     HTLTNRPIVVPGVAEIQITLRSFGDTVFLSPDGQQGVRLENGDQVEARDYGLPVSLIK
                     LPSRSYYEILRQKLKWGER"
     gene            5594..7303
                     /gene="recN"
                     /locus_tag="VF182_17670"
     CDS             5594..7303
                     /gene="recN"
                     /locus_tag="VF182_17670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX6438968.1"
                     /translation="MLREIRIKNFAIIDEVILQLGQGLNIITGETGAGKSIILSALGL
                     ISGERGSSEVIRHKEDEATVEALFETVPPSLGAQLSESGFEIEGELIIKRILNRSGKN
                     RIYLNGSLCPLGLLAQVGASLVHIYGQHEHHTLLQSETHLNLLDAFADLDDKARSMKQ
                     RFEALAHAWNCLADTRQALEQQRREKALLEAQAEEIAKSGLKPGEEEELRSSRNILTH
                     SERLHQGCREGEEILYEGEAALVARLGKYAAKARDLAAIDEGLRPTVELLESSLAQLQ
                     EAALQLRHYADRIHFDPAALEQLDDRLAEIQRLKRKYNGEIEEILRLQTTIQTTLENL
                     QEGEAQILKLQKAFEEARQAAWDAAEKLSGERQRAAKRLKRELEKEIRGLGMPDTIFE
                     VRFRRQDDEEDDPPFFIAGKKLTERGMDQVEFYFSPNPGEPVKPLAKIASGGELSRLM
                     LALKALVLTPGAVSTLLFDEVDTGIGGRVAEIVGKKLRQVAASHQVISVTHLPQIAAL
                     ADSHYVVRKEVDNGRTYTHVERLSEPERVSEVARMLGGVKITERTLRHAEELIEATPK
                     TRS"
     gene            7450..7525
                     /locus_tag="VF182_17675"
     tRNA            7450..7525
                     /locus_tag="VF182_17675"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7483..7485,aa:Ala,seq:ggc)
     gene            complement(7587..>7970)
                     /locus_tag="VF182_17680"
     CDS             complement(7587..>7970)
                     /locus_tag="VF182_17680"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX6438969.1"
                     /translation="VARDAPPEVIRAAYKSLSQKYHPDRNPGDPRASRTMAIINAAYR
                     VLSNPDLRTKHDAWIRRIEAEPAAKPRSISHAAPKPSKPKAPAPQARVFKSKYKIVNH
                     LRRYGWVYLFLALVEFLLIATMLFS"
BASE COUNT         1816 a         2045 c         2270 g         1839 t
ORIGIN      
        1 ggtcggtccg gccggcgcca atcggcctaa tggtattttg ttcttgagaa ctcgacgggc
       61 gcgaatctgg aagctcactg aaactaatgc tcttataccg gtcacatccc gcgatccatc
      121 gcttgggtta gaaaggcaag cttagtaatt caccctatta tacactgatg accatgcctt
      181 ccgccgcgac ggcggtcgca ggaaaacgct gtcgagctaa tttcaatatc cgatcgatac
      241 agcggtcatc gtgggtcggt tcgtgatgaa acaagacaag tcgtttgact ctggcttttt
      301 tggcaacttc agtcgcggat tcaaccgtgc tgtgtcccca gcctcggcgc ggatttgccg
      361 ccgatgtgta ttccgactgc aaatattgtg cgtcatggat caatacgtcg gcgtcgcgcg
      421 cgaactcgat tatctcgggg taaccgcccg gtgtttgctc taaatcggtg gcgtaaacca
      481 cacttcttcc ttggaaaaaa acccgataca aaatcacccc gttgggatgg gcgggactgc
      541 catgggtaag aatgaacggt tgctggctat tgttatccct gagactaacg gtttgggtta
      601 ttcgtggggc ctgccggttg tcgcgcaatt cgatacgttc gccgccaacc agcgaccaga
      661 tcgatttttt cgcgtcaaga gtatcgagcc cgatgggaaa tagcgcaggc tccatcgcca
      721 cgcgcaaagt ttcagccaac gatcgttttg ccgaacccgg cccgaatatg ttgatccggt
      781 tgcgtttatc gaaaagcgga tcaaaaaaat aaaagccgga cagatggtcg tgatgtgtgt
      841 ggcttaggaa gagattcaag ctgagcggac cctggttgtt tttgataagg tctttaccga
      901 gttcgatgat gccggtgccc gcatcaaaaa tgaattggcg cttccccgca actacctcaa
      961 cgcatgaagt atggccgccg tattttagca cgccgcgatt cgagaccgcg taactgcctc
     1021 gcactcccca aaaccggaga gagaaattgc tcgccgccat ctagagtaca cgcccgcaga
     1081 gttcttggtt gtttgccgtt gccagtgaat gtcctttagc agtttgctga gaaaatccta
     1141 tgtcgggctg ctccaaagac ttagacgcgc ggcgcgcaag atttcgcgag ctgagacgta
     1201 cttatgaggg cttttcatca gctgctagaa tgtaccctaa gcaaccctac ggtgacaaac
     1261 ggcggcagag tttcgttcgt ggtattatta tgtcaccaga ctctaatcga gtgccgcagt
     1321 cacggcttag cctgtttagt gccttgcggt gctttatggt gtgctggcaa ggaacgtgtt
     1381 tagcagttag ctgttccgtc caatcataag gagagaacct tggcgcaacg acttcaagcc
     1441 accgacagtc cggacatctt tctcgaggcg ccgccggcgc caaggcacaa gctgcggtat
     1501 tttatattca ccgggctttt tttaattatc ctcagctggg cttttcaggg tgcgaagatc
     1561 agggtcgggg aattgctcga agggataccc cagatagccc agactcttgg gcgcatgtta
     1621 cctcccgatt tttcaaaact gacggacgca agctactatt atttcccgga tgaactctcc
     1681 tggcgcgagc tcttgctacc ggtgccgctg cctgaggcgc tcgcacggac caggcaacgc
     1741 tggtggaaca atatatttcc gcagaccgta tttggggcaa ctctagagac cgtgcaaatg
     1801 gcgctcgcag gaacgttcat ggcgttgatt gtggcgtttc cgttgggatt tcttgcagcc
     1861 cgtaatacct cgccgcatcc agtggtttat catagcgttc gcactgtctt gaatctgctg
     1921 cggactatac cggatttggc gctgggtctc ttgttcgtgg ccgcagtggg gctaggcgct
     1981 tttgccggaa ccctcgcatt ggcgattcac accgccaccg ttctgggtaa actgttgtcg
     2041 gagtccgtgg aaaatattga tgagggcata gtcgaggcga ttcgcgccac cggcgccggc
     2101 tatacgcaga ttctttcctt tgccgttttg ccgcagatcc tgccggattt gatttcattt
     2161 acactgtatc gtcttgaaac caatattcga gccgcctccg tgcttggttt gattggcgcg
     2221 ggcggcattg gttacttgat gaatactagt tttcgcacct tccaatatca ggaagcagca
     2281 gccatcgtgc ttgttctgat cgcgctcgtt atgctagtgg attacctcag ctcgcgttta
     2341 cgaaccctgg tcgtgtgaca gcccagaagg aaagctaaca atggatttga aagttgtcgg
     2401 acgccccagt cgccgtgtcg acggaccgga taaagtcagc ggcaacaccg tctacaccgc
     2461 cgatgtgcaa ttgcctggca tgttgtgggg cagatgtttg cgcagcccct acgctcatgc
     2521 ccgaatcctt tcgatcaaca ctgaaccggc aaaaagagtg aaaggtgttc gcgcggttct
     2581 taccggcgag gatttaccgg ataagcgggt cggattgagc ctgcaagaca cgccgcttct
     2641 agcgatcggc aaggtacggt tcatcggtga gaaggttgtc gccgtggccg ccgaagatcg
     2701 cgacgccgcc gaagaggccc tctcggccat agagatcgag tatcaggagc taccgggtgt
     2761 gttcgatgcg ggtcaggcga tgacccgcga tgcgccgttg gttcatgagg atttggccac
     2821 atacgcgggg tttaaaacgc cggacgttga aatcccgaac gtttttgcga tgcagaaatg
     2881 gactgcggga gatatttcga cgggcttcga tgaagcggac ctggtcctcg agcatacttt
     2941 tagaaccgca gttgcgcatc aggggtatat cgaaccacac tctgcgatcg tggccgtgga
     3001 tagctcggat cgtgtcgata tctgggcgtc ctgcaaggcg ccctttcgag tcaagtccca
     3061 cctgtcgcgc cagttagata ttcctaaaga gcggatccgc gtccatgtgg ttgcggtcgg
     3121 aggtgatttc ggcggcaagg gcgccttgat ggacattccc atttgctacg agctggccaa
     3181 ggccgcccgc ggaccggtaa aaatgatcat gacctatgcg gaagagctaa tcgccggcga
     3241 tccgcgccat tcctcggtga tcaagatcaa aagtggggtc aagaaagatg gtactcttac
     3301 agcgcgtgaa accgaagtaa tcttcaacag cggcgcatat gcatcgttta agcccggaga
     3361 tccgccgaat ctcggcggcg ctacttttac caatggtgtc taccacatcc caaattattg
     3421 cattcggtcc tacggcgttt acaccaattc tgtgccttgc ggctattatc gcgctcccgg
     3481 ccaaccccag gcggtgtttg ccgccgagtg tcacatggat ttgatcgctc aagagttgcg
     3541 catggatccg ctcgaattgc ggctaaaaaa tctcctgcgc aacggtgaga agttagctgg
     3601 cggccgggcg gtggacaagg tgcgggtgcg cgaaactctg caggcggcgg cgcagaaagc
     3661 gggttgggga aaaaagaacg gcaaccatac cggtcttggc atcggcgtat cgttccgaca
     3721 tgttggcggc tccggtaaag cgagcgcgga gctttctctt tctgccgacg gtaaactcaa
     3781 gatcttaaca gccgtgcccg atatcggaac gggaacacat accttactac ggcaagtcgc
     3841 cgcggaagtt ttaacaattc agcctgagct gatttcgacc gaaatcggcg acaccgaaag
     3901 ctttgaaagc gacgccgcgc caggaggcag taagattacc aatatgagcg gccatgtcgt
     3961 gctgcaggcg gctgaacagc tgcgccagcg attgacgccg tttgcagctt cgcttcttgg
     4021 ctgcgctgtg actgacgtag ttctgcacaa cggccgcttc ggcagtgctt tggataagaa
     4081 gcgttcgatt gaatttagcg cggtggcgcg ggaggcgact aagtcaaacg gtgatctgcg
     4141 tgtgcgtgaa accttcgagg taaaaggccg ctcgccggtg ccggcctttg tcgtgcaaat
     4201 cgccgaggtc caagtggatt tcgagactgg ccagctgcgg gtaaaacgga tcgttacggc
     4261 acacgatgtc ggcacggtga tcaaccccgt ggcgcatcaa ggccagatcg aaggcggttt
     4321 ggttcagggg ttgggttacg ccactctcga ggaggtgatg accgacaacg gcaaagttct
     4381 gaccaccaat ttaggcgact accgcattcc atgcgcgcgg gatctgccag ctttggacac
     4441 cgtgctcatc gaggacccga acgggccggg gccatttgcg gccaagcaga tcggcgagaa
     4501 tggcattatc gccaccgctc ccgcgatcgc caacgcagtg gcagctgcgg caggtgtgag
     4561 gctgttcgat attcctttga ctgccgagaa aatttaccgg gcgttaaaat cgtcctcgcc
     4621 tgccgcatag gctgctagca cggcctgata gtaaaccatc gttaatcacc gtaaaccgat
     4681 gagacaggtg tttgcgcagt gggaaaaaag ttcatttggg gagaagcaac ttcgatgatc
     4741 cgtgctgtgg gattagtggc aaaatatcac gagcccaaag ccacggagat ggcccgttgg
     4801 ctagtccctt ggctaaagaa gcgaggcctg aaggtttacg tcgaaaatgg catttcccgc
     4861 agcggtggta tttcttctac caagaaagac atggccatca aagctgattt gatcatttcg
     4921 ttgggcggcg acgggacttt acttaatatt gcgccgttgg ttgaaaggcc cgatgtgccg
     4981 attctcggag tgaatctcgg cggcttggga tttatcaccg aagttgcggc cgacgaactc
     5041 gagtcagtgc tgacacatac gttggagggt gaataccaga cagagaagcg catgactctg
     5101 gaagtgcagg tccgaagtaa aaacggaaaa gctcatcggt tccgcgtgct gaatgacgcg
     5161 gtaatcacca aaggtgcgcg cagccgaatc atcaatttgg aaacttatgt cgacggcgag
     5221 tacttatgta attatcgcgc cgacgggttg attatttcga cgccaaccgg ttcgaccgct
     5281 tattctctgg cggcaggcgg gccgattttg gcgccggcgc tcagcgcaat aatcctgact
     5341 cccctgtgcc ctcatacgtt gaccaaccga cccatcgtgg tacctggtgt cgcggaaata
     5401 cagattacgc tccgctcttt tggagacacc gttttcttaa gccccgacgg acagcagggt
     5461 gttcggctcg agaacggcga ccaagtcgag gcgcgcgatt atgggctgcc ggtatcgttg
     5521 ataaagttgc cgagccgcag ttactatgaa attttacgtc agaagctgaa gtggggggaa
     5581 cgttaagccg gtcatgttgc gcgaaatccg gattaaaaat tttgccatca tcgacgaagt
     5641 tattctgcaa cttggccagg gtctcaacat cattaccggc gaaaccggcg cagggaaatc
     5701 cattatcctt agtgcactgg gacttatctc cggggagcgc ggcagttcag aggttatcag
     5761 gcacaaagaa gatgaagcaa ctgtcgaagc cttattcgaa accgtgccgc catcactcgg
     5821 cgcccaattg agtgaaagcg gtttcgagat cgagggcgaa ctgatcatca agcgcatcct
     5881 taatcgttcg ggtaaaaacc gcatctatct aaacggtagt ctttgtccct tagggttgct
     5941 agcccaggtg ggcgcttctc tggtgcatat ctacgggcag cacgaacacc ataccttgct
     6001 gcaatcggaa acccacttaa acctactcga cgcttttgcc gatctcgatg acaaggccag
     6061 atctatgaag cagcgcttcg aagcgctagc ccacgcttgg aattgcctgg ctgatactcg
     6121 ccaggctttg gaacagcagc gccgggaaaa agcgcttctc gaagctcagg ctgaagagat
     6181 tgctaaatcc ggcttgaagc ccggcgaaga agaagagctg cgttcgagca gaaatatttt
     6241 gacgcactcg gaaaggcttc accagggttg ccgtgaaggc gaggagatcc tttatgaagg
     6301 agaagccgcg cttgtcgccc gcttgggtaa atatgccgcc aaagcgcgcg acctggcagc
     6361 tattgatgag ggattgcggc cgacggtcga gctactcgaa tcttcgctgg cgcaactgca
     6421 agaggcggcg ttgcaactgc gccattatgc cgatcgaatt cattttgatc ccgccgcatt
     6481 agagcagctc gatgatcgac ttgcagagat tcagcgtcta aaacgcaagt acaacggcga
     6541 aatcgaagaa attttgcgtc tacaaacgac aattcagacg acactggaaa atctccagga
     6601 aggcgaagca cagatcttga agcttcaaaa agccttcgag gaggcgcggc aagccgcctg
     6661 ggacgcggcg gaaaaactct ccggtgagcg gcagcgcgca gccaagcgat tgaagcggga
     6721 attggaaaag gaaattcgcg gcctaggaat gcccgacacg attttcgagg tgcgcttcag
     6781 acgccaggat gacgaagagg acgatccgcc gtttttcatt gctgggaaaa aacttaccga
     6841 gcgaggtatg gatcaggttg agttctactt ctcaccaaat cccggagaac cggtgaaacc
     6901 gttggccaag attgcttctg gcggtgaact ctcccggctt atgctcgctc tcaaagcctt
     6961 ggtacttacc ccgggagctg tctcgaccct tctcttcgat gaagtcgata ctgggatcgg
     7021 cggcagagtg gcggagatcg tcggaaaaaa gctcaggcaa gtggccgctt cgcatcaagt
     7081 catcagcgtg actcatctgc ctcagatcgc cgcgctggcg gattctcact atgttgtgcg
     7141 caaagaagtt gataacgggc gaacctatac tcacgttgag cgcctttcgg aaccggagag
     7201 agtttcggaa gtggcccgga tgctgggagg cgtcaagatt accgaacgaa ccctgcgaca
     7261 cgccgaagaa ttgatcgagg caactccgaa gacccgatca tagaaggtag atgaaaaatc
     7321 gtgattacga ttcgaaaagt ttgcgaattg ctgatttgac ttttgcaaat cgtttattga
     7381 tattaccaag atagcgaggc ggttcgcagc aaaaatgatt cgtctttttc gctcttgcat
     7441 cgttcttgtg gggccgtagc tcagttggga gagcgcttga atggcattca agaggtcgtc
     7501 ggttcgatcc cgatcggctc caccaaactc gtcaaggaaa ataatctgtt tcagttgagt
     7561 tttctgtgat gagccgctga cgtgctttat gaaaacagca ttgtcgcgat caagagaaat
     7621 tcgactaggg ccagaaacag gtaaacccaa ccataacgcc ttaggtgatt gacgatcttg
     7681 tatttgctct taaacacccg ggcctgaggc gctggagctt tgggttttga cggcttaggc
     7741 gctgcatggg atatggaccg aggtttcgcc gccggttctg cttcgatcct gcgaatccac
     7801 gcgtcatgtt ttgtccgcag atcaggattc gaaagaaccc tgtaggccgc attgatgatc
     7861 gccatggttc tggatgccct cgggtcaccc gggttccgat cggggtggta cttctgggac
     7921 aaggatttgt aggcggcgcg gatcacttca ggcggcgcat ctcgtgctac
//
LOCUS       DASUVS010000331         3784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1887277, whole genome shotgun sequence.
ACCESSION   DASUVS010000331 DASUVS010000000
VERSION     DASUVS010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887277"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VF182_17685"
     CDS             complement(<1..647)
                     /locus_tag="VF182_17685"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438970.1"
                     /translation="MKNGFKALDSDMHVYDPPDLYLNYMDSKWGERIPRGEWPKGHGR
                     VEFRLGDGTVLRPRTERIRSGEEKVAKHHTEGPNRGYDPVSQVEAMEREGLDVAVLFR
                     TSPLYADDSFEPEYAAALCRAWNSWIADFCKHNPVKLKPSAVIPMHDVDLAVKETRRA
                     VKELGALGLSIGPEPVRGRQLHDRYFDPLWAEAQDLDVAVCFHPPASPGSEQASK"
     gene            complement(703..1647)
                     /locus_tag="VF182_17690"
     CDS             complement(703..1647)
                     /locus_tag="VF182_17690"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6438971.1"
                     /translation="MGTTLVTGAGLVGTSFGQVALKRGENLVYFDPQPRREFLDMKLG
                     RGNFQVVQKDVRDLPALIEAIHSHKAQTVVHTAGLIGSRVAESLYTGFHINVVGTMNV
                     AEAVRLTGVKRMVHISTFGVYDWRKEASEPVTEDFYKGSGVPYGNSKVAKELLLEAYQ
                     RMHKFELVVIRPANVYGLGHFWSGSGGGEKIQALLVSGLKGETAKIPEEQTMAFEYIY
                     CKDLGRAVDLAATVPQPEKNTFNIGEGKVTRFEEIVATVQKLLPKCSVEIVPGTPPVS
                     RTQHLDISRAKKYLGWEPQYTMEQGFKDYVEDLRKTMS"
     gene            complement(1681..2802)
                     /locus_tag="VF182_17695"
     CDS             complement(1681..2802)
                     /locus_tag="VF182_17695"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438972.1"
                     /translation="MAKNGFKAFDSDMHVYDHPDLYTKYMNPKWGERVPVAKGWNKHG
                     RPQFSIGGGTKLRPREALLDYGEQRVEERYGFALARGYDAVSQIQAMDMEGLDVAVLF
                     RTSPLHCDETFEPEYANDLCKAWNSWIADFCRVSPKRLKASALITLHDVDLAIEEVRR
                     AVNKLGVAALSLCPEPINGKFIHDRHFDPLWAEIEKLGVALCFHPPARPKQDQVANKF
                     FGHPNGDIVALALRNPVELILAVSEFCAGGVLEKFPKLRVAFLEGNCAWLPWLLYRLD
                     ERAKLHGPLADVPLSKKPSEYFFQQCFISVDPDEYLVTDVISRIGDNNILISTDYPHI
                     DAAFPHALDEFFAIEQMNDASRRKILWDNCVRLYGVTDS"
     gene            complement(2973..>3784)
                     /locus_tag="VF182_17700"
     CDS             complement(2973..>3784)
                     /locus_tag="VF182_17700"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438973.1"
                     /translation="RYGLTADIIYISSGPVVLQALIGGDLTVGLGASNSTVAAALRGA
                     PVVAIGGIINKAYHRLFVHPEINRIDDLRGKTLGVTRFGSVTDNLTQILLRKYNLEGK
                     VNVRQMGGVIEVGAAFQQKLIAGAVSSELRTSEHHPAKVLIKLVDLGIPYSMDMIVVS
                     RDYLRQQPATAERILRAYMEGVAALHQQKERALKVIAKYGRLEDPKKIEAHYQDSVDY
                     LEKLPRIDADAVATIVDFMGKKDTPMEAFADKAIIDRLAREGFVDKLYGKR"
BASE COUNT          839 a         1111 c          972 g          862 t
ORIGIN      
        1 ctcttggacg cctgctcgct gccagggctc gccggcggat gaaagcaaac tgcgacatcg
       61 agatcctggg cttccgccca caagggatcg aagtaccgat cgtgtagctg ccggccacgc
      121 accggctcgg ggcctatcga aagaccgagc gcgcctaact ctttcacagc gcgccgggtt
      181 tccttaaccg ctaaatcaac atcgtgcatt gggatgacgg ccgacggctt cagcttcacg
      241 ggattgtgtt tgcaaaagtc cgcaatccag ctattccaag cccggcataa cgccgcagca
      301 tactccggct cgaaactatc gtcggcgtag agcggcgagg tacgaaacaa caccgcgaca
      361 tccagcccct cacgttccat ggcctcaacc tgcgagaccg ggtcatagcc gcgattgggc
      421 ccttcggtgt gatgctttgc gaccttttct tcgccgctgc gaatccgttc cgttctggga
      481 cgcagaaccg tgccgtcgcc caagcgaaac tctacgcgtc cgtgaccttt gggccactcg
      541 ccgcgaggaa tccgctcacc ccatttgctg tccatatagt tcaagtagag gtccggcgga
      601 tcatacacgt gcatgtcgct atctagagcc ttaaatccgt tcttcatgct tttcttcttc
      661 ctcacttcag acggcggact cgttgattca ttccgacggc gttcacgaca ttgtcttcct
      721 gaggtcctcg acataatctt tgaatccctg ttccatcgtg tattgcggtt cccagccgag
      781 atatttcttg gcccggctga tgtcgagatg ctgggtgcgt gagactggcg gcgttcccgg
      841 aacgatttcg accgagcact tcgggagaag cttttgcacg gtagcgacga tctcctcgaa
      901 tcgcgtaact ttgccctcgc cgatattgaa agtgtttttc tcgggctggg gaacagtcgc
      961 cgccaggtca acggcgcgac ccaggtcctt gcagtaaatg tattcgaagg ccatggtttg
     1021 ttcttcggga atcttcgccg tctcgccttt aagcccgctc accagcaaag cttgtatttt
     1081 ttcaccgccg cctgaaccgg accagaaatg gccaagcccg taaacattag ccggacggat
     1141 tacgaccaat tcaaacttgt gcatccgctg gtaagcctcc aacagcagtt ccttggcgac
     1201 tttggaattg ccataaggca cgccgctccc cttatagaag tcctccgtga ccggttcgga
     1261 agcttcttta cgccagtcgt aaacaccgaa ggtactaatg tggaccattc gtttcacgcc
     1321 ggttaaacga acagcttcgg cgacattcat cgtcccgacg acgtttatgt gaaatccggt
     1381 gtacaaggat tccgcgaccc tcgaaccgat caaaccagcc gtatgcacaa cggtttgggc
     1441 cttgtgcgag tgaatcgctt cgatcaatgc cggcaggtcg cgtacatctt tttgcaccac
     1501 ctgaaagttc cctctgccca gcttcatatc gagaaattcc cggcgcggtt gcggatcaaa
     1561 ataaaccaga ttttcgcctc gtttcagcgc gacttgtccg aatgaggttc ccaccaatcc
     1621 agcgccggtg actaatgtgg ttcccatttc tcctccttga aattagatct caggtaaaga
     1681 tcacgaatcc gtgacgccgt agagccggac gcagttgtcc cagagtattt tgcggcggct
     1741 ggcatcgttc atttgttcga tcgcgaaaaa ttcgtcgagc gcatgtggaa aagccgcatc
     1801 gatatgggga tagtcggtcg agattagaat attgttgtcg ccaatgcggg aaatcacatc
     1861 ggtaaccagg tattcgtcgg gatcgacgga gatgaaacac tgttgaaaaa agtactcgct
     1921 aggctttttt gataacggta catcggccag cggaccatgc aactttgccc gctcatcgag
     1981 ccggtaaagc agccatggca accaggcgca gttaccttcc aggaacgcca cccgaagttt
     2041 gggaaacttc tctagaactc cgccggcgca aaactccgac accgctaaga tcaattccac
     2101 cggattgcgc agcgccaggg cgacgatatc gccgtttgga tgaccaaaaa acttattggc
     2161 gacttgatcc tgctttggtc ttgccggcgg atgaaagcaa agcgcaacac cgagcttctc
     2221 gatctccgcc cacagaggat cgaagtggcg gtcgtgaatg aacttgccgt tgatcggctc
     2281 gggacaaagc gaaagagcgg ccacgccaag cttgttgaca gcgcgccgca cctcctcgat
     2341 tgccaaatcg acatcgtgca gcgtgatcaa agccgacgcc ttcaaacgct tggggctaac
     2401 ccggcaaaaa tccgcgatcc agctgttcca tgctttacac agatcattgg catattccgg
     2461 ctcaaaggtt tcgtcgcaat ggagcggcga ggtacgaaac aacaccgcaa catcgagtcc
     2521 ttccatgtcc atcgcctgaa tctgcgatac cgcgtcgtaa cctctcgcga gagcgaagcc
     2581 ataacgctcc tcgacccgct gttcgccata atcaagcaac gcctcgcgcg gccgcagctt
     2641 ggtcccgccg ccgatggaaa actgcggccg cccgtgcttg ttccaccctt tcgctacagg
     2701 aacccgttcg ccccacttcg gattcatgta cttggtataa agatccgggt ggtcgtagac
     2761 atgcatatcg ctatcaaacg ctttgaagcc gtttttggcc ataactacct ccttgagcca
     2821 gatcgcgatc tcccgcgctg tgcattagac atccaatgaa tttaaattac tggaacatgg
     2881 gaacagaaac ttacgaaaac aacttgtccc gccttcattt gacaagcggc tgcagccccg
     2941 ggccgttgca gacacgcgaa aaacgaaaag tctcatcgtt taccatagag tttatcgacg
     3001 aaaccttccc gcgccaggcg atcgataatc gccttatcgg cgaacgcctc catgggcgtg
     3061 tctttcttgc ccatgaaatc cacgatcgtt gccacggcgt ccgcatcgat acgcggcaat
     3121 ttttccaagt aatcaaccga atcctgataa tgcgcctcga tctttttcgg gtcctcgagg
     3181 cggccgtact ttgcgatgac tttcagggct ctttcttttt gctgatgcag ggcggcgact
     3241 ccctccatgt aagcgcgcag gatacgctcc gcggttgccg gttgctggcg aaggtaatcg
     3301 cgcgagacca cgatcatatc catcgaatag ggaattccca gatcgaccag cttgattaaa
     3361 accttggcgg gatgatgctc gctggtgcgg agctcggaag acaccgcgcc ggcgatcaac
     3421 ttttgctgga acgcggcgcc gacttcgatg acgccgccca tctgacgcac gttaaccttt
     3481 ccttcgagat tgtatttccg caacagaatc tgggtaaggt tgtccgtcac cgagccgaat
     3541 cgcgtcactc ccaaagtctt gccacgcaaa tcgtcgatac ggttgatctc aggatgaacg
     3601 aagagccggt ggtaggcttt attgatgatg ccgccgatgg cgaccacggg agcgcccctg
     3661 agtgcggcgg caaccgtgga gttactggcg ccgagtccga cggttaagtc gccgccaatg
     3721 agcgcctgta acaccaccgg tccggacgag atatagatga tgtccgcggt gagtccatat
     3781 cttt
//
LOCUS       DASUVS010000332        36948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_576648, whole genome shotgun sequence.
ACCESSION   DASUVS010000332 DASUVS010000000
VERSION     DASUVS010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 36948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36948
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576648"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..1843
                     /locus_tag="VF182_17705"
     CDS             326..1843
                     /locus_tag="VF182_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX6438974.1"
                     /translation="MPHQDRLNIAVFIDFDNIEIGVKSTLGENFDIGAILDALKERGE
                     VVTKIAYADWTRAGEYSRHLTQHAIRLVQRNLTPGGDKNGADINLALDALEMAFTHPH
                     INAYVIVGGDSDFLSLVEKLKQYDKKVFVVGGRAFTSIILQRNCHEFIAYENLTGGRR
                     SASKGGRPVAPAASSPIAQAFPIVRRALKILSDREVSPQTGLLKSTLLQLDSTFSERN
                     YGASSFHDFVEKLAQAGFAQLKHTGRSVMVELNEQFEEGDQSAAAPAKPQAAPETRGD
                     VLRPPSPEAEAVEGPVVAAVPVTPAGDQADGVRLVGQILTAATTARWPIYLRNVKQLL
                     RSADGGFDERRYGFAGLIDLLRACQREGLVRLERDRRGGLLVFKGPVLQQTEATRSAV
                     PQQDPGEAESEDVVESMDAGAREQLEELNQGEPDNRLEPQAEAVDDKAAEIGPIPTDT
                     TAEPLGRAKPSRPRARAGAAARSTRKTTAPKKPAARATTRSRKTVRSDSGGKNES"
     gene            complement(2099..3121)
                     /locus_tag="VF182_17710"
     CDS             complement(2099..3121)
                     /locus_tag="VF182_17710"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438975.1"
                     /translation="MNHARMQHAKSCRFVLAAVAALLLGPMPTWAQERPLKKINWGQT
                     ALSAAMWIPWIAKEAKIYEKNGLDVEIVLLRGSGQSSQALLSGSLFAAPVALPQLMLA
                     DLNGADMVNVAHTIASASSKLLVRPEIKTAADLRGKRVATSSIGSLGDFLFRYALRKH
                     GINPDREITWISIGTNAERLQALLTGVIDGADVTYPADIQAERKGYRVLIDARKEIVF
                     PTTSVVTTRRNIREDRDTVMRMVRSHVEGIAFHKQNKEFALKVLTKYVRTTDREFLEG
                     SYDIFKQDFIPAPYPITKGLEPTYDYVAQTRPDIRNHKPEEFVDASFITELDRSGFIK
                     KLYEGK"
     gene            complement(3282..4061)
                     /locus_tag="VF182_17715"
     CDS             complement(3282..4061)
                     /locus_tag="VF182_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6438976.1"
                     /translation="MTPDPYPAFDGVEPYGIVRSVVDERAHRKRMCALGYRIFGALRW
                     GMLGDGHISARDPELTDHFWLLDWGVPFAEATVDQLVLVGPGGEVKDADGDATGAVNT
                     AGYNIHHPLLAARPDVVSASHTHTAFGTPWSANVEPFRALSQEACAFVFDQALFDDEE
                     VEVLSIDGGQRIAAALGKKKFCILRNHGLLTVGGSVEESVGWFVMAERVAEVHVKAPN
                     GRPISDEAAALAASTLAADSVGWRMFQWLQRSLVSDRSVVD"
     gene            complement(4120..5388)
                     /locus_tag="VF182_17720"
     CDS             complement(4120..5388)
                     /locus_tag="VF182_17720"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX6438977.1"
                     /translation="MLSKEDNEFACRVGPGTPMGELFRRFWLPAMLPDELPHADCPPV
                     RLRLLGEDLVAFRDSNNKIGVVEAHCPHRGASLYFGRNEECGIRCVYHGWKFDVDGNC
                     VDMPSEPPESNFKDKIRITSYPTVFHGGLIWVYMGPQHLRAELPQLEWTQVPDSHRSV
                     SKWFQETNYLQGYEGDIDSSHASFLHTYLNPAASPNEGTTIDPSLKKRDGAPKIVVND
                     TDYGFRYGARRIVGEGTYNWRVTQALLPTYSLIPFMRFPAGGRAWIPVDDHRTMTFYI
                     SYHPDRPLTDDDLEIRRTGRAFPPELIPGTFIPKRNMENDYLLDREIQRTTSYTGIWG
                     VNDQDRAIQESMGPIYDRRKEHLGSSDLAIITARKRLLNLARELQQGIEPFQASRGDI
                     YRVRAMDVNTPVDNFDAMIASHGSGLRAKA"
     gene            5997..6392
                     /locus_tag="VF182_17725"
                     /pseudo
     CDS             5997..6392
                     /locus_tag="VF182_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(6815..7300)
                     /locus_tag="VF182_17730"
     CDS             complement(6815..7300)
                     /locus_tag="VF182_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6438978.1"
                     /translation="MMKPAKNTVCLWYDRDAEEAARFYAKTFPDSSVGAVHRAPGDFP
                     SGKKGDVLTVEFTVMGIPCLGLNGGPAFKHNEAFSFQVATADQDETDRYYNAIVGNGG
                     QESACGWCKDKWGLSWQITPIALTKAVTDPDTAAAKRAFDAMMQMKKIDIAAIEAARR
                     G"
     gene            complement(7866..8834)
                     /locus_tag="VF182_17735"
     CDS             complement(7866..8834)
                     /locus_tag="VF182_17735"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX6438979.1"
                     /translation="MNGTVEPVGDEGLEKRLRSQSSRIYVESAIGALILAVLSFFFAS
                     VLPEAVRSFPDLSLEQAADFLIRHGYVVLFGWVLLEQLGLPIPAAPLLIAAGALARAG
                     KMNLAFTVALPFVAIILADLFWYSLGRYRGGRILKLLCRITLEPDSCVRRTENVFIRH
                     GVRSLLVAKFIPGLNTAAPSLAGVFRMPMRRFLIFDSLGGLLWVVTVTSLGLILGDQL
                     ERIALRWGGWLVAVLAGGLAAYVLWKFIQRRRFLRRLRIARITPKELMDKLTAKENIS
                     IVDLRQPMDIEAFPQMIPGALRIAMEEIEERHGEIPRDRDVVLYCS"
     gene            complement(8865..9071)
                     /locus_tag="VF182_17740"
     CDS             complement(8865..9071)
                     /locus_tag="VF182_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438980.1"
                     /translation="MNVEKAELANIGPKETRKRLLMGLAMLALGIALAVVFAHADVSR
                     GWYAALFLPFWIGTLALFQARKKT"
     gene            complement(9554..10462)
                     /locus_tag="VF182_17745"
     CDS             complement(9554..10462)
                     /locus_tag="VF182_17745"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438981.1"
                     /translation="MRIAWAGSTPSNTPIWVADQKGFFKKNGLNAEVISISASTIVIQ
                     ALLTGEVDFIIAPSATLVTSRLAGADTIMVSTNLPLFIDHIVSLSEITTVEQLKGKTG
                     GVNRLGTTSDMTLRLALRRFGIDPEKETKIIATGENPQRLAALSRNITQFTLLGEPLV
                     REAEKMGFRDLFDIGKLKIPYHVNAVVTREKTVKERRPLVNRVVRAFTEAIHYIKTNK
                     EDTKALIGKNLKSNDPEGLERAYLAYNAAYPEVPYPSAEGVKTLLDDIAPRTPKAATA
                     DPKTFVDMSFVQELETAGFIKQLYKR"
     gene            10767..11753
                     /locus_tag="VF182_17750"
     CDS             10767..11753
                     /locus_tag="VF182_17750"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438982.1"
                     /translation="MNPSARSIVYLLTITVLIAGFSSDGAAQSRTKIRYALGDVISID
                     ELPLLIAAEQSKQRGVDVEVTSFKSEEVATQAVINGQADVGQGTPYAAVQKLSVPIRF
                     FYQLSSLLFYPVVNKESYKTWKDLDGQEIAVQGRGSGTEAIMMLAAKEHGIKYKNVSY
                     VPGSQVRALALLKGNIRATILDAPNKNLVMKDGADKFIILPLGNVKASDEALFAMKEF
                     LDKNQAPVRIFIEELTKVARAINANPKWVLEQRKKLGLLKDLPAKMEEEILPFFQEAV
                     ENGVYPNDGGLGNAAKNDLEFFYLSGALKGENLKAEDFWDLAPLKSALANVK"
     gene            11943..12710
                     /locus_tag="VF182_17755"
     CDS             11943..12710
                     /locus_tag="VF182_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6438983.1"
                     /translation="MQRIKPIANHVILQLLSFVLFFGVWELAGRWPISLAFPPFSETF
                     LALMRMVADGSLLKAYASTLQPLIIGIVICGIAGIAFGIFMGLSRSIEWLSLPVFIIL
                     QAAPMAAMIPLITYMYGIGLTAKVLAVVLLAAPVIVMNSYKGIRNTNPSLIQMCRAFL
                     GTRRQEVFKIILPYAAGLIFAGLRLGLAMGFIGIVIAELMITPTGIGDLITYHSSVAG
                     YAEMFAAIGSIVVVAALTIYALERLERKLFPPEIRES"
     gene            12714..13469
                     /locus_tag="VF182_17760"
     CDS             12714..13469
                     /locus_tag="VF182_17760"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6438984.1"
                     /translation="MEPIVEVRNIAKTYDSGVEALRDVTLALPRGELSTFLGPSGCGK
                     TTLLKIIAGLISPSEGEVWVKGNKVSGPGPERAFVFQDFALLPWASVLRNVAFGLELR
                     GVPRDEREEIARKYITEVGLSSFESRYPHQLSGGMKQRVGLARALAIDADIVLMDEPF
                     SSVDEQTRRKFQEELLDLLKINQKTVIFVTHSIEEAAYLSDQIILLSQRPGSVLKIIR
                     PNIERGESADEIRRDKGYLDTVDEIWKILRDYV"
     gene            13497..14249
                     /locus_tag="VF182_17765"
     CDS             13497..14249
                     /locus_tag="VF182_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6438985.1"
                     /translation="MIFFGKRIPLFFSLLVWFFLWELVGRAEYSIIIPPVSRVIVAGF
                     TILPTEKFAAAASISLRSFALGMALAIAIGLPVGILMARLENLGKVLSLWVNIFVSAP
                     ISALVPILMSVIGIGETTVIVTVFLFAVFVIILDTQVGIKQADKSLVEMAFSFGARRG
                     QIYTKVLLLSALPEILAGLRLGAIRGVKGVVIGQLLIAILGVGELFELYSRNFLMEEF
                     WALVIVVFLFAFAVSEAIAFFERRVEYYASAR"
     gene            complement(14276..15286)
                     /locus_tag="VF182_17770"
     CDS             complement(14276..15286)
                     /locus_tag="VF182_17770"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6438986.1"
                     /translation="MTQCKSFFLLIFFLVTMAWFMPLFAQTIDIDAAKKEARVVVYAA
                     VPPQTMKVINDPFEKKYGIRVEYWRASTTGILERALNEWRVGQAGADVIEGNKGPQLI
                     LKSEGVFDKFIPPSATKFPQQFVEADGILTPWRFNPISILYNTELVRKEELPKTLSDL
                     LLPRWKGKITIPDPSRHTTTAQFLADLDRYEGINWREFAKGLSQQQPLMVESFAPIVN
                     TVIKGEANLGITYLKYVGQYKGPIDYVRLNKYLADTNYMGVSRKAAHPNAGRLYVEYI
                     CSPEGQKAMAATGEFVLAPQISPAFPGASDVAQRTLFMEEPSAEEFKKLREEMRQIFL
                     AK"
     gene            15394..16437
                     /locus_tag="VF182_17775"
     CDS             15394..16437
                     /locus_tag="VF182_17775"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6438987.1"
                     /translation="MPLLIRAEDVNGLLTMEEAIDAVEAGFREWGKSPELNAPRKRIM
                     TPEGVRVSVHPGGIPSLGGIGVLAHSEHVAVSKEVQTYHNMGGPVTVLFDVKDASLMA
                     ILVGRVALKELPAERPTPIRTAATSAVGTRSLARADSKILGILGAGGEAKYHLLALAR
                     TRPFKTAKVYCRTQSTREQFCRSMEKLVSCEVAPASSAKEVVESADVLLTATNSNVPV
                     FSGEWLQSGAHVTSIMGGNVGLVRAGVAARPRRELDDTTIRRSDVIVVNSREQAMQDE
                     QADLFGPVQAGFLRWEDVGELGHLLNSVIPGRTRANQITLFKNNAGQGVADVAVASKI
                     YSLARSNGIGMEF"
     gene            16620..17600
                     /locus_tag="VF182_17780"
     CDS             16620..17600
                     /locus_tag="VF182_17780"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6438988.1"
                     /translation="MNIDLHSHFFPIEALQNPGKYESRAPKMALDKGKLSVTSQIGFR
                     PGLGPGAYDPSARIEALNQMGIDLQAISPSPILLFYWEEPPVAAHFSRKQNEAIQDIV
                     ERHPDRFVGFGSVPLQSIADSIEIAREAKAMGLKGLEIGNAVGDKPLDDPIFEPFFNA
                     AQELDMLLFVHPLEGGLDADDPLSPILGNVLQFTFRTTMMVERMIVKGMFEKYPNLRL
                     CLSHGGGLLAFNIWRLDHSYSLRPELKKIIPDKPSAYLKKLYFDTIVHSTAALEYLVK
                     VVGADRVVIGTDYPMGMGDFEAVAKIMQSDLPAAQRELILGANAARALSM"
     gene            17728..18666
                     /locus_tag="VF182_17785"
     CDS             17728..18666
                     /locus_tag="VF182_17785"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX6438989.1"
                     /translation="MKASPPTVSILVDLDPGSADRTVIVESITADKQGRLYLPDRVSG
                     NILRVDPKAPKPEVVGRIESREIKGKKVDANASGVAFNQQGDLFIAVGPFSEVVRIRG
                     TELNPQKPGSAQTFATGTPGANGIAFDRSENLFISGGGSGIIYQVGPEGGAAQAAVQV
                     EKHARTLPDGSQQATVANGLDFDGNGVLHVADTARGAIWKVVIGPDGKGGKPSLLAQS
                     PLLEGADGLAFDGRGNLWVTANERNALVSVTSAGAVREFSKNGSQGPLEFPAAIVFVG
                     DTAYIANYDTPRRDNMDTNGKTALDGIGASIAQITR"
     gene            18809..19585
                     /locus_tag="VF182_17790"
     CDS             18809..19585
                     /locus_tag="VF182_17790"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6438990.1"
                     /translation="MSLLSIEGVRKEYQVRGKRLVALESVDLDVREGEFVTIVGPSGC
                     GKSTLLNLIVGLLRSSGGRIVFRGAAIDAICTQIGYVTQKDNLFPWRTLVENVEIALE
                     IRGVARVLRRREAAELIERVGLRGFEDHYPHELSGGMRQRANIIRTLIYDPELILMDE
                     PFGPLDAQTRIVLQDELLKLWSTTKKTIIFITHDLIEAITLADRVVLMSSRPGRIKAI
                     EPVNIARPRDVFKIHESEQFRAVYENLWRELRPEVRLAEA"
     gene            19603..20616
                     /locus_tag="VF182_17795"
     CDS             19603..20616
                     /locus_tag="VF182_17795"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438991.1"
                     /translation="MKRLMKIICAATLGCLGTSLSADAQTPRKVKMTIPVVAHSMTPV
                     YIAQSKGFFAEEKLEPDITSTGGGGPDIRALIAGDVEFSFTTGDNVILAQQEGKRLLM
                     VMSALNKLFINWAMHKEAAKSKGVTESMPLADRMKSLKGLTIGVTNPGALTAHLAAFV
                     VRKAGYNPQQDVNIVPVGAGPTWLAALENRKVDVALTAPPVPETAISRGFAIMFINNA
                     KGEDPSIPEFLMENLIARPETVAKDPDLVRRMVRALTRANQWALKSTPEQVADALKPF
                     MAKTPPDLLLAGAAAVLPTLSSDGRVSERSFQTTQEILEQAGMLKKRVSYSELVTNEF
                     LAK"
     gene            20613..21398
                     /locus_tag="VF182_17800"
     CDS             20613..21398
                     /locus_tag="VF182_17800"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6438992.1"
                     /translation="MISRSRLFVYRLIFGLFLLAGWELSSGRVIDPFWVSSPSKVFGY
                     VYEVFADGSIFAHLGITLYETFTGFFIGSIFGIGLGFVLGRREILADILDPYVVAFNG
                     IPRIALAPLFIIWFGIGPTSKVILVISVVFFLTFFNTFAGVKGVDAELKNVLRIMGAS
                     ERQILMKVTLPAIVPWITTGLKIALPYAIVAAVVGEFIAASKGLGYLINYNTSLFSTT
                     GALGGILILALVVVLCNEGISRAEAYLLRWRPREEKKREGELY"
     gene            21533..22420
                     /locus_tag="VF182_17805"
     CDS             21533..22420
                     /locus_tag="VF182_17805"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6438993.1"
                     /translation="MRSKHRIQRKELPVAIRGGLWMAGAAAAFTVMTTLIREAAKGVH
                     PFEIAFFRALVNLVLMLPFALRTGSAGLRTNNHKAFALRGVCGLAFLMTYFTGAALIP
                     VAESQALTFTSPLWGTLLAVTFLHERLTLARALAVIVGFAGVLVILRPGAVQVSLGAV
                     LVLGGALSAAGSNTIVKFTTRTDHPDTVVFYQMVYVTPLIFVPALYIWTWPALDQLLL
                     MIGVGFFATLNQRFLSRAYASADAIAVLPFEFARLPFAAVIGFLAFRELPEVWEWIGG
                     AVIFSASLYMVRQEARATR"
     gene            22514..22987
                     /locus_tag="VF182_17810"
     CDS             22514..22987
                     /locus_tag="VF182_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438994.1"
                     /translation="MSYRWSKSKDGWIGRAVVFLSQVGCGASQQAGGSLVVMLAVDWS
                     FNSSALLRKALHATTVMAMAADMVRPAPEQPNLRNTMIRSSHARRRLKKFNMFVPSLQ
                     TGEVLLAAKTFSNGCAQQFQHQVVDAREKAQDYRKLAIHERVGWRELKTGLLGQS"
     gene            complement(23220..24233)
                     /locus_tag="VF182_17815"
     CDS             complement(23220..24233)
                     /locus_tag="VF182_17815"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6438995.1"
                     /translation="MISSRGYRQIISAIALVLIGLATGNAHSQESIRVAYSSTDALNS
                     LWTIAADAGFYKKHGLDAEVVYIGSTTVAVSAIVAQDVQIGNGAGSGVANAAVRGADI
                     VSVACFINTLPYELVVLDNIKSAEDLKGKSIGISRFGSVSDVAARELLKGLGLKPMED
                     VKILQVGGASERAAGFSRKIIAGFPSPPGNVHLIPGGLPHRILANMADSEKPYPLPFI
                     CAVTTKRYLAKNRPVVKRAVMALIETGHFFKTNKDATQKLIAKYLRGANKAYLDSAYE
                     STAKLIERVPYTTREGMKIQLEDALKQNPGSKMTVDNLIDDSIVREIEKEGFIDKVYG
                     KRG"
     gene            complement(24385..25266)
                     /locus_tag="VF182_17820"
     CDS             complement(24385..25266)
                     /locus_tag="VF182_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6438996.1"
                     /translation="MSVYLKIILCAAAITVLDGTAQAQQQPPFATTKVDGTENVYVFR
                     YGGAQAMFVVTKEGVIATDPIGYGRPQAATTYLEEIKRVTNQPVKYVIYSHHHFDHIA
                     GGKPFKDAGATFIAHKRAKERLEQLKDPHTVIPDETVDTHRTITLGGTTLELTYLGLN
                     HSDSTLVMRLPAEKIIFTVDWLPVGSLPGRGMIDSYPLEWEDSIKKVLAIDWERLIPG
                     HPGAGGRLGTKNDAQEMLTFLQETSAEVKKAAHEGKCWDTVEKEMKLPKYASMPGYEQ
                     ALPFILRRYCGLWGRGT"
     gene            25585..28674
                     /gene="glnE"
                     /locus_tag="VF182_17825"
     CDS             25585..28674
                     /gene="glnE"
                     /locus_tag="VF182_17825"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF010707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEX6438997.1"
                     /translation="MLWVVTRSSSVFDRKCWLMKTQRKNPISSSHAKIQQLLKKSPSP
                     AHALANLGRLIESGGSQLIDKIPSTELPLLTRLLGSSAYLSEVLIGRGENWPELFLEQ
                     IKVKRKSVDDHLSDLKAVTTAAQSLDEFCAGLRRHKQREYLRIGARDLDPSAPLEETV
                     EELTSLAEASLESAYLYCRRETEREVGHLNLPGADKPNGFVVLGMGKLGGGELNFSSD
                     VDVIFLFENDEGESSGGPKGKAEPRAFFAAIGKKIIQAMGKVTEDGFVFRIDLRLRPL
                     GVNGPLVQSVDSAMLYYESWGQCWERAALIKARPVAGDRHLGDRFLKEVEPFVYRRYL
                     DYTTVEELRHMKMRIENELLSPQDKERNIKLGYGGIREIEFFTQALQLVNGGYEPALR
                     HQNTLRALSELAAHKFISADEMNQLAEAYRFLRQVEHKVQMVQEAHAHSIPQGEEEET
                     ALARRLGYLPQPKRSEREQFWSAKNIHSENVRRTFDRLFYGAQKEISHDGQSHAGTIW
                     HDLDQREVIIRELEKLGFSDANKAYENLLAVRDGESFAPPSAKRLKVMRTLGPALIAE
                     IARSGAPDQALLNLSRFSHRIGGRSGFLRLLAENPETMRLLITLFADSQFLTELFLTR
                     PELIDTLIRVDLTRIDKSKESMMAGLREAVAEQDDIEGKLNALRRSKTEEFIRIGLHD
                     LGGSIDLEPVLQQLSDLADAVVECALQLALEELQEKFGIIPQGRFAVLGMGKMGGREL
                     DYNSDLDLVFVYAADEDASSSGGPQGKLPAHDFYVRVGQKLITYLSAPTEEGIAYKID
                     MQLRPSGKAGPLVSSLEAFREYHRAGSQLWERQSLIKARYVAGDSSLGITLEKLAADV
                     AYGSGLSSEGVGEIHHLRMRMERELAGESDSRFNLKKGRGGIVDIEFLTQMLQLAHGI
                     HHPGIRCQATLKALKALHQQGILKKKEYATLADGYLFLRRLDHRLRLERDQSIDAVER
                     DPGRLDGIARALGYDSAVTPRAKNLAKAGRKLLADYESRREKIRACYERFFVGANNE"
     gene            28867..29265
                     /locus_tag="VF182_17830"
     CDS             28867..29265
                     /locus_tag="VF182_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6438998.1"
                     /translation="MKTQIYKGLALASFVVVMSAPLHAQTTAPADQSPEQPVTAELGY
                     GVASALASVFYIPAKVTYAGLGLVTGGLGYVLTGGRSDVASNIIYPAVRGHYVVTPNQ
                     IKGIDPIYFVGAPPESPEPESYAAGTPATR"
     gene            complement(29262..30035)
                     /gene="lpxA"
                     /locus_tag="VF182_17835"
     CDS             complement(29262..30035)
                     /gene="lpxA"
                     /locus_tag="VF182_17835"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX6438999.1"
                     /translation="MSIHSTAVVDSRAEVDSEVEIGPYVVIEGPVRIRRGTRIMAHAY
                     LTGWTEIGENNEIHPGAILGGAPQDKAYQGQESYLTIGSHNVIREHAQVHRGTAPNSS
                     TIIGNHNYLMATSHVGHNCKLGDHIVMANGALLGGYVDVGNSVFISGNCVVHQFIRIG
                     EHAMMRGLSGTSRDVPPFAIIDWQHTVRGVNVVGLKRAGFDDRRIRAIRNAFRVLFRK
                     GRNLSVALREVEESMRADDDVMTLVEFIRSSKRGVCFGA"
     gene            complement(30050..30244)
                     /locus_tag="VF182_17840"
     CDS             complement(30050..30244)
                     /locus_tag="VF182_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007016585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEX6439000.1"
                     /translation="MPLHVKCPTCRKECSWDDNPHRPFCSDRCQLIDLGAWTEERYRI
                     PGRKVETQLGEDEENEKENR"
     gene            complement(30255..31232)
                     /locus_tag="VF182_17845"
     CDS             complement(30255..31232)
                     /locus_tag="VF182_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-dihydroxyphthalate decarboxylase"
                     /protein_id="HEX6439001.1"
                     /translation="MAKADLTVALSHYDRHIPLFDGSIQVEGANVTVLEVGQSHPLKH
                     GQDRHERMLQKQEFDICELSLSSYLMAKSRGLPFTAIPVFPRRLFSLSQMWVNVDAEI
                     TSPDDLPGKRVGLSTFQTTLSVLAKGDLQSEYNVPWRKIEWVVSKAEAVEFKPEAGVH
                     IELLAPGKKIGVLLEHGEIDALMVPHPPAEALEGSKKIRRLFPDPKREEIKYFQKNGY
                     YPIMHVVAFKDDVLKRDPALAVSVMTAFDQAKMACKAYYDDPNWSRFVWGRHLFEEER
                     KIFGDDPWPHGLEKNRANLERFIDYSKDQGLLAKKLSVDELFASSTLNS"
     gene            complement(31515..31712)
                     /locus_tag="VF182_17850"
     CDS             complement(31515..31712)
                     /locus_tag="VF182_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439002.1"
                     /translation="MPSDETRRLLRTFGVAVTQYEDAVSSGAPAEEIQKAEREARSCL
                     SEITALLDRLKTGGGTTDVAT"
     gene            complement(31744..33543)
                     /locus_tag="VF182_17855"
     CDS             complement(31744..33543)
                     /locus_tag="VF182_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX6439003.1"
                     /translation="MHGFHGRILKIDLTTASYTSLEIDSSRLRRSLGGIGLGADLLYQ
                     FAPPGIDALAPENPLIFASAPLVGTPLTTTAKYAVVTKSPLTSFIADSLSSSYFALEL
                     KRLGVDALVITGRSPAWTYLSIDRGRVSFGDATHLVGKSAVETDSFLRAESNKDNFRV
                     AAIGLAGENGVRFATISNDGRHAGRGGVGAVMGAKRLKAIAIRGDMDTAIADGRGVAE
                     LALAMRHKSVGTVTSKYRQIGTVANLAVFNRLGSLPTRNFQHSTFAHAENLSGESLTE
                     NNFSRRNGCAACTIRCERLFKSLDGQDQRLEYETLFALGPMCEIEDPHTLLQAARLCD
                     DYGLDSISTGATIAWAMECAEKGLLPEATHLGLRFGRDDAFLKIIPMIAERRGLGDLL
                     AQGSKRAAAIVGKDTGQWAMHVKGLELPGYEPRSLKTMALGLAVSPRGACHNRSGAYE
                     FDFSGEVDRLRGDAGRGLLVAASEDFAAVLDSLIVCKFLRKCFTDFYAEAADLLAKVT
                     GWPYSSAELRQIGERINAQKKLFNLREGWQPEDDWLPPRLLDEKLADGVAQGTELRPA
                     ELHEMIQSYYLARGWNDRGFIPESKMRELKISH"
     gene            33811..34386
                     /locus_tag="VF182_17860"
     CDS             33811..34386
                     /locus_tag="VF182_17860"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX6439004.1"
                     /translation="MELSRRGFLRYSVAAGGATALSGLIGSGVNLGPAIARAQELRIK
                     GAKATPSICPYCSVGCATLVHTIDGKIVNIEGDARSPYSEGTLCPKGAAIFQLHVNPN
                     RPTQVLHRAPGASDWEVWDLERAMDRVAELVKKTRDETFIEKLENGKLVNCTTSIFSL
                     GGATLDNEWNHIQQKLMRGLGIVPIENQARI"
     gene            34456..>36948
                     /locus_tag="VF182_17865"
     CDS             34456..>36948
                     /locus_tag="VF182_17865"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6439005.1"
                     /translation="MAESDCCVFMGSNMAENHPVAFRWPMKAKANGAKLIHVDPRFTR
                     TSAMCDIYAPIRAGSDIAFLGGLVNYVINNERWNKEQFFQDFLVHYTNAPMLISDEFQ
                     DTEDLDGVFSGLMQYKEKLNEWPYNGFLGEYNNQSWQYAGTQAETQGWTGDTARSGEQ
                     QVGKEQAPPGGNQAAGAKDGEAQKAAAKGPPFEPLVRALLKPPPRRDETLKNPRCVFQ
                     IVKRHFSRYTPEMVERVTGCPQATFLKVAETILANSGPDRTTSFAYAVAWTQHTNGPQ
                     VIGCCALLQLLLGNIGRPGAGIMALRGHASIQGSTDVPTLYHSIHGYMSHPTALKKHD
                     TLGDYLVAETKTRSYWANLPKFMVSYMKSMYGDAATAENGFGYDWHPKIIGDHSHMAM
                     FAAMAEGKVRGMLLIGQNPATSLNAKLERAGMRRLEWLVVKDNWVHESATFWKNAPEI
                     KNGEVNLTDIKTEVFFFPSTQVAEYEGSFTNTFRMLQWHYKAADPPGDCRSDLWFTHQ
                     LAKRLKKLYGESNEPRDAGFKNLTWDFDPDERSEGLRAQTAPAQAEGGAVGGATTGGT
                     APSAAGSSGAAPGAAKDQPPGSAPAPGTPQHNPGEPDGLKILREINGYYTGDPNKHVA
                     GFADLKDDGSTTCASWIYSGVFPKPGENRSANRKPDPPDAPGGAQLGWGYSWPANRRI
                     MYNRASADLQGKPWSERKKYIWWDGQKWTGYDVPDFAATKPPTAKAQPMGIGLDAQDG
                     TDPFIMLDGGVGWLYVPTGLVDGPLPTHYEPAESPVQNPLYKQQSSPVLKYWKTSGNP
                     LAPASDAKFPHVITTYRLTEHYLAGAMSRWNPW"
BASE COUNT         7968 a        10299 c        10533 g         8148 t
ORIGIN      
        1 ggacggagat gtagactggc caacgaacat tgcttgggag acgccacgcg agtggctgcc
       61 ctcacgtgat caaggtgaga gcacggttcg cagtttggac agatctattc tggattacct
      121 cgcgggtttt cctcgatcag aaaggcaatt gacaactctc cggtccggcg ttactgctcg
      181 gccagagcaa aagctggttt gaatttctta tgcatacgcg cgatcatgca gtgtggccaa
      241 ttcacgcggg tgatcgtccc gcccctcgag gcgccggcgc ccctctcggc acaaaataaa
      301 aaatagaaaa ttgaaaggat ttcagttgcc ccatcaggac cgtctcaata tcgccgtctt
      361 catcgacttc gacaacatcg aaatcggtgt caaatcgacg ctcggagaga atttcgacat
      421 cggcgctatc ctcgacgcct tgaaggagcg gggtgaggtg gtgaccaaga tcgcctatgc
      481 cgactggacg cgcgcgggcg agtacagtcg ccatctcacg cagcacgcca tccggctggt
      541 gcagcgcaat ctgacgcccg gcggcgacaa gaacggggcg gacatcaacc tcgccctcga
      601 cgccctcgaa atggcgttca cgcacccgca catcaacgcg tacgtgatcg tgggcggcga
      661 cagcgacttc ctctcgctgg tcgagaagct gaagcagtac gacaagaagg tcttcgtcgt
      721 gggcggccgc gctttcacga gcattatcct gcagcgcaac tgccacgagt tcatcgcgta
      781 cgagaacctc acgggcggcc gtcgctccgc gtccaagggt ggccgtccgg tggcgcccgc
      841 agcctccagc ccgatcgcgc aggcgttccc gatcgtgcgc cgggcgctga agatcctgtc
      901 cgaccgcgaa gtgtcgccac agacggggct cctgaagagc accctgctcc agctcgactc
      961 gaccttctcc gaacgcaact acggcgcgtc ctcgttccac gacttcgtcg aaaagctggc
     1021 tcaggccggt ttcgcgcaat tgaagcatac gggccggagc gtgatggtcg aattgaacga
     1081 gcagttcgag gaaggcgatc agtcggcggc cgcgccggcc aagccccagg ctgcgccgga
     1141 gacgcggggg gacgtccttc gaccgccttc gcccgaggcg gaggccgtcg agggacctgt
     1201 cgtcgcggcg gtacccgtca cgccggcggg tgaccaggct gatggtgtcc ggcttgtcgg
     1261 gcaaattctg acggccgcga cgaccgcgcg gtggccgata tacctgcgca atgtcaagca
     1321 gttgttgcgc tcagccgatg gcgggttcga cgagcgccgc tacgggtttg ccggcctgat
     1381 cgatctgttg cgtgcctgtc agcgcgaggg cctcgtgcgg cttgaacgag atcgccgcgg
     1441 cggcctgctc gtcttcaagg ggccggtcct gcagcagacg gaggcgaccc ggtctgcggt
     1501 cccgcaacag gaccccggcg aagcggagtc cgaggacgtc gtcgaatcga tggacgccgg
     1561 tgcgcgcgag cagctggagg aacttaatca aggcgagccg gacaatcggc tggagccgca
     1621 ggcggaggcc gttgacgaca aggcggccga gatcgggccc attcccaccg acacgaccgc
     1681 ggaacccctc gggcgcgcca agccgagccg tccgcgcgcc cgcgccgggg ccgccgctcg
     1741 gagcacgcga aagacgacgg cgccgaagaa gcccgcggcc cgtgcaacca cccgatcgcg
     1801 gaagaccgtc cgctccgact ccggcggcaa aaacgagagc tgacgcgcag aggagagggt
     1861 tcctgtaagg ccagattttt gggtctcgac tatcttgttc agccattccc gtcctggtgt
     1921 atcatcggct tgcaaccgat cgctcggcgg tgcgaccgct caggccagga gggtctggcc
     1981 tgagcatcga gttatagtcg tgactaccaa cttcaggatc ggacaaattc tcgatttgaa
     2041 aacagcgggc agagacatag cgtggtccga cccgtgacag gcgatcgtat caatgtcctt
     2101 acttgccttc gtagagcttc ttgataaacc cgctcctgtc gagttccgtg atgaaactcg
     2161 cgtctacgaa ttcctcaggc ttgtggttgc gaatgtcggg acgggtttgc gcgacgtaat
     2221 cgtaggtggg ttcgagacct ttggtgatcg gatatggggc agggataaag tcttgtttga
     2281 agatatcgta agagccttcg agaaactccc gatccgtggt cctgacgtat ttggtcaaga
     2341 cctttagcgc gaactctttg ttctgcttgt ggaaggctat gccttcgacg tgggagcgaa
     2401 ccatgcgcat gaccgtgtca cggtcctccc ggatgtttct gcgggtcgtc accacggacg
     2461 tggtcgggaa aacgatttct ttgcgggcat cgatcaaaac acggtagcct ttgcgctcgg
     2521 cctgaatatc ggcaggatag gtcacgtcgg cgccgtcaat cacgccggtc aacaacgctt
     2581 gcagtcgctc cgcattggta ccgatcgaaa tccaggtaat ctcgcggtcg ggattgatgc
     2641 cgtgcttgcg tagcgcatag cgaaacaaaa aatctcctag cgagccaatg ctcgaagtcg
     2701 ccactctttt gccgcgcaaa tccgccgcgg tcttgatttc cggtcgaacc agcaatttgc
     2761 tgctggccga agcgatcgtg tgagcgacgt tgaccatatc ggcgccgttc aggtcggcaa
     2821 gcatcaactg cggcaaggcc accggggcgg caaacaaact cccgctcagg agcgcctgcg
     2881 agctctgtcc agagccgcgc agcaaaacga tttcaacgtc caagccgttc ttctcgtaga
     2941 ttttcgcttc cttggcgatc cagggaatcc acatggccgc cgacaaagcg gtctggcccc
     3001 agttgatttt tttgagcggc cgctcctgag cccaagtcgg cataggtccg agcagcaggg
     3061 ccgccacggc ggccaaaaca aatcgacagg attttgcgtg ctgcattctg gcatgattca
     3121 tagtcgtttc cattttcatt tcggtgcgta cagctttttg attaaatccg cagatcagga
     3181 agtcacccat taatcttggc gagcaagcca ctccggtggc tggcgatcac ggcgtcgaaa
     3241 ttgtcggccg gcatgttcac gtccatggcc cggcgcgctg ttcagtcgac cacagagcga
     3301 tcggagacga gcgaccgctg gagccactgg aacatccgcc atccgacgga gtcagcggca
     3361 agtgtcgatg ccgcaagcgc agcggcctcg tcagagatcg gtcgtccgtt tggcgctttc
     3421 acatggactt cggccacccg ctcggccatg acaaaccatc cgaccgattc ctccaccgag
     3481 ccgccgaccg tcagcaggcc gtggttgcgc aggatgcaga atttcttctt cccgagcgcg
     3541 gcggcgatgc gctggccgcc atcgatcgat agcacctcca cctcctcatc gtcgaacagt
     3601 gcttggtcga acacgaaggc gcacgcttcc tgactgagcg cgcgaaacgg ctcgacgttg
     3661 gccgaccatg gcgtgccgaa ggcggtgtgg gtgtgagatg cgctcacgac atcggggcgg
     3721 gcagcgagca gcggatggtg aatgttgtaa ccggccgtgt tgacggcgcc ggtcgcgtcg
     3781 ccgtcggcat ctttcacttc gccacccggc ccgaccagca ccaactggtc gaccgtcgct
     3841 tcggcgaatg ggacacccca gtcgagcagc cagaagtggt cggtcaactc ggggtcacgg
     3901 gctgatatat gaccgtcgcc gagcatgccc cagcgcagcg caccaaagat gcggtatccg
     3961 agcgcgcaca tccgtttgcg gtgcgcccgt tcgtccacaa ccgaacgtac gatgccgtag
     4021 ggctcgactc cgtcaaacgc tggataggga tccggtgtca tgtcacgcat cgtatttcgc
     4081 atagcagcct ggatgcagag tttttcaaca gtctgttagc tacgccttag cgcgtaagcc
     4141 gctcccgtgg ctggcgatca tcgcgtcgaa gttgtcgacc ggcgtgttca cgtccatggc
     4201 gcgcactcga tagatgtcac cgcgcgacgc ttggaagggc tcgatgccct gctggagttc
     4261 acgggcgaga ttcagcaacc gcttgcgcgc cgtgatgatc gcgaggtcgc tgctgccgag
     4321 gtgctccttg cggcggtcgt agatggggcc catgctttcc tgaatcgcgc gatcctgatc
     4381 gttgacgccc cagatgccgg tgtagctggt cgtgcgctgg atctcgcggt cgagtaggta
     4441 gtcgttctcc atgttgcgtt tcgggatgaa cgtgccgggg atcagctcgg gaggaaatgc
     4501 gcgaccggtg cggcgtattt cgaggtcgtc gtcggtcagc ggccgatccg gatgatagga
     4561 aatgtaaaag gtcatggtgc gatggtcgtc caccgggatc caggcgcgtc cgccggccgg
     4621 gaagcgcatg aacggaatga ggctataggt cgggagcaga gcttgggtca cgcgccagtt
     4681 gtaggttcct tcgccgacga tgcgacgggc gccataacga aagccgtagt cggtgtcgtt
     4741 gacgacgatc ttcggtgcgc catcccgctt cttcagactg ggatcgatgg tggtgccttc
     4801 attcggggaa gccgccggat tcagataggt gtgcaggaac gaggcatgag agctgtcgat
     4861 gtcgccttcg tacccctgca ggtagttggt ctcctggaac cacttcgaca ccgagcgatg
     4921 actgtcgggg acttgagtcc actccaattg cggcagctct gcacggagat gttgcggtcc
     4981 catgtaaacc caaatcaggc cgccgtggaa gaccgtcggg taagacgtga tccggatctt
     5041 gtctttgaag ttgctttcgg gaggttcgga cggcatgtcc acgcagttgc cgtcgacatc
     5101 gaatttccag ccatgataga cacagcgaat gccgcactcc tcgttgcgcc cgaagtagag
     5161 agacgcgccg cggtgcgggc agtgagcctc gacgacgccg atcttgttgt tggagtcacg
     5221 aaacgcgacc agatcctcac cgagcaggcg caggcgcacc ggcggacaat ccgcatgggg
     5281 caattcgtcg ggcagcatcg ccggcagcca gaatcgccgg aatagctcgc ccatgggcgt
     5341 tcccggcccg acacgacacg cgaactcgtt gtcttctttg cttaacacag cgcctccgtg
     5401 tattgggaag aatctaaatc actttttttc tcatcgatga tttatcgctg aaccggcgaa
     5461 ggtgtcaatt gtcggagagt taaataaaat taggcgtgat gcgtgggcgt aatcttcttt
     5521 gcagttcgcc cgccacgcat ttgcaataca cgccggcgct gatcgcttcg ttggatcacg
     5581 aacagcagag aacgacgtcg cgatcccgcg ccgtcgcgag gtagatttgc agatcgtcgc
     5641 gaccacttgc gcggtcaggc atcgccagct gtcaatggcg atgccatctc ccgccgctcc
     5701 attttcggaa atgggattta taatgaataa tgaaaatcga aaacgtgctt cgaaggctaa
     5761 gcctgccgca tgcaggctca ccccgtttta agtggaatcc ttcttgactc aagcgttctc
     5821 ggtgctatcc gttccctcag atgatgttcg gttggactat cacccaacac gaaaccaaac
     5881 agtgaagtga taccatgcgt tagataatcc tggttgtttt gaccagcctt gaaaatatcc
     5941 aaacttaagt agggcaaagc aatgcgatca caacaatgcc aacaaggagc gtgactatgg
     6001 ctgttcgtct gaatcacaca attgtcgccg cgcatgacca tgaagcgtcg agcaaattct
     6061 tggccgagat acttggtcta ccacctccgc tgcagttagg gccgtttgcg atcgtaacgg
     6121 taggtgacac ttcgctggat tatatgaacg ttaaagagta ccgaggcgcc gatggcgaga
     6181 tcgtttctca acatttcgcc tttctggtaa ccgaggccga attcgacgag atcttcgcgc
     6241 gcattaggga acggcacctg ccttattggg ccgatccgcg gcgcagccag ccggagcaga
     6301 tcaacgagtg ggatggcggc cgcggagtct atttcgtaga tccgaacggt catgtgctcg
     6361 aagcgattac gcgaccttac ggcagcggtg gcacaacggc ggctcgaccg cacccattgc
     6421 tcgcgtcaac cccgaacggg gcacctgaaa ctcacaatga tcacaccgtg agaggttgaa
     6481 ggcgctcatc tggcgaccgc agaacggtcc gatgagtgcg ccgcatgaaa gacttcatga
     6541 gcgtaaaaga acgataacgg gaaccagcgg gccagagcag atagttggcg gcagcaagtc
     6601 cgaccggtag tcacgcatta agccacctat gcatgtggcg cctcttcact actcggctgt
     6661 ctaacgtaaa gcccttgagc ggtcgcggcg gccgacgcac gctcgttcga ccgccagcga
     6721 actatacagc cgccagcggc gggttatatg gcatgtcgcg cgaccgcggg acgaacttcg
     6781 atagaaaggc atgcacgctt caacgattgc aacttcaacc gcggcgcgcc gcctcgattg
     6841 ccgcgatgtc gatctttttc atctgcatca tcgcatcgaa cgcgcgtttg gcggcagcgg
     6901 tatcgggatc ggtgacggct ttcgtcaggg cgatcggcgt aatctgccag gacaaccccc
     6961 atttgtcctt gcaccagccg cacgcgctct cctggccgcc gttgccgacg atcgcgttat
     7021 agtagcgatc cgtttcgtct tggtcagcgg ttgcgacctg aaacgagaac gcttcattgt
     7081 gcttgaacgc aggtccgccg ttgagtccga ggcacggaat gcccatcacg gtaaactcga
     7141 cggtcaacac gtcccctttc ttcccggacg gaaagtctcc cggtgcgcgg tgcacggcgc
     7201 cgacagacga atcgggaaag gtcttggcgt aaaaccgcgc cgcctcctcg gcgtcgcgat
     7261 cgtaccaaag gcaaaccgtg ttcttcgctg gcttcatcat gtcacttctc catcgagatg
     7321 agccagccga ggtcggagtc aacgatgtgc tcgcagtcaa agggcattgg ctcggtccac
     7381 tcgcgtcaat gggctaaaat atatatggct aggccaatca aaccagcatt ccgtcggaat
     7441 gtcgagcgac ttctgcaaca taatgcaatg ctcattcgcg cgtattccgc ggcgctctca
     7501 agcagaaggt tagacgatcc tcacctttta ggcatgggac ccaccctctt gcctaaagtg
     7561 taaacggccc atcggcaccg ccgcgtagaa catgctgccg ttattttgta tcactccttc
     7621 acgacgatct tatgaacctg atggggtctc tcaattaggc catctcgacg aacccttcat
     7681 tcacttcgta tctggcgcga gaatatcttc tctcagggga caattggttt cttggcgagg
     7741 ctttcggtaa cgaatgggaa atcgtccgcg agccaagcgt cgatcccgcc ctcgagcggg
     7801 cgcaccctgg tgattccttt ggttcgcagc agcagcgcca cacgggcgct ggtcgcttcg
     7861 ttgggtcacg aacagtagag aacgacgtcg cgatcccggg gaatctcccc gtggcgctct
     7921 tcgatctctt ccatggctat gcgaagggcg cccgggatca tctgaggaaa cgcctcgata
     7981 tccatgggct ggcgcaagtc cacgatggag atgttttctt tggccgtgag tttgtccatg
     8041 agttctttcg gcgtaatgcg cgcaatccgc agccgccgaa gaaagcgtcg tctctggatg
     8101 aacttccaca aaacataagc tgccaaacct ccggccagga ctgcgaccag ccaaccgccc
     8161 cagcgcagcg cgatccgctc gagttgatcg ccgagaatca agcccaggct tgtgacggtg
     8221 acgacccaga gcaaaccgcc cagggaatcg aagatcagga aacgtcgcat gggcatgcgg
     8281 aagactcccg ccagagacgg tgccgcggta ttcagaccgg ggatgaattt ggcgacgagc
     8341 aaagaacgga cgccatgccg gatgaaaacg ttttcagtcc gccgcacgca cgagtcaggt
     8401 tcgagagtaa tgcggcacag tagcttcagt atccgcccgc cgcggtaacg gcctaaagag
     8461 taccagaaca ggtcggccaa aataatcgcg acgaacggca acgccacggt gaaagccaag
     8521 ttcattttgc cggcgcgggc cagcgcgccg gccgcgatca acagcggagc agccgggata
     8581 ggcagaccca gctgctcgag caacacccac ccgaacagca cgacgtagcc gtgcctgatc
     8641 aggaagtcgg ccgcttgttc caggctcaaa tccgggaagg aacggaccgc ctcgggcaat
     8701 acgctcgcaa aaaagaaact gaggacagct aatatcagcg cgccaatcgc cgattcgaca
     8761 tagatccgac ttgactggga gcggagtcgt ttctcgaggc cttcatctcc gaccggctct
     8821 acggtgccgt tcatcttgcg cgcgccgcgc gccgccaaag cgactcaggt ctttttgcga
     8881 gcctggaaaa gagcgagagt tcctatccag aacggcagaa atagcgccgc ataccacccg
     8941 cggcttacat ctgcgtgggc gaagacgacc gccagggcga tgcccagcgc gagcatggcc
     9001 aaacccatga gcaggcgttt acgggtttct ttcggcccga tattcgcgag ctccgctttt
     9061 tcgacattca tcgaaagctg cccgtacctt tgttcatttt ttgatctccg attgagtttc
     9121 acccttaata gccaacattc gtacatccgg ttagagtgcg ccgatgcagg caaggcatgc
     9181 ttcttctttt atagatggct gtgacgaaaa atgcatgttc cggcccgggc ttgcagtcgc
     9241 ctgagcacga gaacttttat cgaccgccca actccgatgc cagggctttc tggtacggtc
     9301 ggtcgagagc catcagcgcg ccgtaaaaac gtcgggctaa gtctgagata gcagggcata
     9361 gggtcgggcc tcgcgttgac acattccgga tcaccatgtg gtcaacgtga gagcatccgt
     9421 tctgcggtag ttccaaacgc tttaggcgtt aattgcggca cgatcttcct tcttgcagtc
     9481 acaacagctg ctcggtttcg tcgcgagcgt tttacgcgtt gcgaaaagcg gacattgtga
     9541 aagcctgtgc cgctcaccgt ttatataact gcttgataaa tcctgccgtc tccagttcct
     9601 ggacgaaact catatcgacg aaagtcttgg gatcggcggt cgcggctttt ggcgtgcgcg
     9661 gcgcgatgtc atcgagcagt gtctttactc cttcggcact tgggtacggc acctccggat
     9721 aggcggcgtt ataggcgaga taagcgcgtt ccaaaccttc cggatcattg ctcttgaggt
     9781 ttttgccgat gagcgctttg gtatcttcct tgtttgtctt gatgtaatga atcgcttcgg
     9841 tgaaagcgcg cacgacccga ttgaccagcg gccgccgttc cttgacggtt ttctcgcgcg
     9901 tgacaacggc attgacgtgg tacggaattt ttaatttgcc gatatcgaat aggtcccgaa
     9961 accccatttt ctccgcttct ctgactagcg gctcgcccag caaagtaaat tgcgtgatgt
    10021 tgcgcgacag agcggcgagg cgctgcgggt tttccccggt tgcgataatc tttgtttctt
    10081 tttccgggtc gatgccgaaa cggcgtaagg cgagacgcaa cgtcatatcc gaggtagtgc
    10141 ccaagcgatt aaccccgccg gtttttccct tcaattgctc aaccgtggta atctccgaga
    10201 gcgaaacgat gtgatcgata aatagcggca aattagtcga gaccatgatg gtatcggcgc
    10261 cggctaaacg ggatgtgacc agagtggcgg acggggcaat gatgaaatcc acctcgccgg
    10321 tgagcaaagc ctgaataacg attgtgctgg cgctgatgct gatgacctcg gcattgaggc
    10381 cgttcttctt gaagaaacct ttctgatcgg ccacccagat cggcgtgttg gacggtgtgc
    10441 tgccggccca tgcgattcgc atccgttctt gagctacggc cggcgcacac ggagctagcg
    10501 tgagaagaag aatgagcaac ataagtctcg cgcataaagg catgagcaaa ccctccgctg
    10561 agcgccggtg attttggtta accagtgagt gtactggcca accatggaaa ggtcgggcga
    10621 gttatagcgc tgctccgttt tgactgtcaa tcatgatgaa tagatcgaat cacattgacc
    10681 agctcacgtt attaaaagca gcttgacagc cacccggagt ttgggatact cagccggcaa
    10741 gttaaagttg aggcaggagg tgacgcatga acccgagcgc gagatcgatt gtctatcttc
    10801 tgacgatcac cgttttgatc gccggcttct ccagtgacgg cgccgcgcag agcaggacca
    10861 agatcagata cgccttgggt gatgtcatct cgattgacga gctgccgctt ctgattgcgg
    10921 ccgagcaatc aaaacagcgg ggtgtggacg tggaggttac ttcatttaag agcgaggaag
    10981 tggcgaccca ggccgtaatc aacgggcagg ccgatgtcgg ccagggcaca ccgtacgcgg
    11041 cagttcaaaa gctctcggtg ccgatccggt tcttctacca attaagctcg ctgctcttct
    11101 acccggtagt caacaaggaa agctacaaga cttggaagga cctcgatggg caggagatcg
    11161 cagtgcaggg tcgtggttcc ggaaccgagg cgattatgat gctggcagcc aaggaacacg
    11221 gcatcaagta caagaacgtg agttatgttc ctggctccca agtccgcgcc ttggctctgt
    11281 tgaaaggcaa catcagagcg accatcctcg atgcgcccaa caagaacctg gttatgaagg
    11341 atggcgccga caaattcatc attctgccgc ttggcaacgt caaagccagt gacgaagcgc
    11401 tttttgccat gaaagagttt ctcgataaga atcaggcccc ggtcagaatt tttatcgagg
    11461 agctgaccaa agtggcgcgc gccattaatg ccaatcccaa atgggttctt gagcagcgca
    11521 aaaaactcgg attgctcaaa gatcttcccg ccaaaatgga agaggagatc ctgccgtttt
    11581 ttcaggaggc ggttgaaaac ggcgtctacc ccaatgacgg cgggctggga aacgcggcaa
    11641 aaaatgatct tgaatttttc tatctttcgg gcgcgcttaa gggtgaaaac ctgaaggccg
    11701 aggatttctg ggacctcgcg ccgctaaaga gcgctctcgc aaacgtgaaa tgattgactc
    11761 ctcgcggcgg cgaagccagt agtttgccgc atgatcgagt atcgtcgcgc agccttatgg
    11821 caccgcctca gtccagcgtc ccaatgctgc tctggagaga aggcagccat taaccgcaag
    11881 cctggtgctt gcggttgaaa gtgacggttt tccggaaatg ccgccaatca actgctgatc
    11941 ctgtgcagcg catcaaaccg atcgccaatc atgttatttt acagttgctg tcttttgttc
    12001 ttttcttcgg cgtttgggag ctggctggcc gttggccgat cagcctcgcc tttccgccct
    12061 tcagcgaaac gttcttggcg ctgatgcgca tggtcgccga tggtagcctt ctgaaagcgt
    12121 atgcctcaac cctccagccg ctgatcattg gcatcgtcat ctgtggaatc gccgggatcg
    12181 ccttcggcat tttcatgggg ctctcacggt ccatcgaatg gttgagcttg ccggttttca
    12241 tcattctaca ggcggcccct atggcggcga tgatccctct gatcacctat atgtacggaa
    12301 tcggtctgac cgcgaaggtc ctggcggtgg tacttctggc agcgcctgtg atcgtcatga
    12361 attcctacaa aggcatacgc aatacgaacc cctcgctgat ccaaatgtgc cgggcgtttc
    12421 tcggaacgcg gcggcaggag gtatttaaaa ttatccttcc gtatgccgcc ggcctgatct
    12481 tcgccggact gagactgggg cttgcaatgg gattcatcgg catcgtcatc gctgagttaa
    12541 tgattacacc gacaggcatc ggcgatctga ttacttatca tagctcggtg gccggctacg
    12601 cggagatgtt tgccgcaatc ggttcgatcg tggtcgtggc ggcattgaca atttatgcgc
    12661 tcgagcggct ggagcgaaag ctgtttccgc ctgagatccg agagtcttga cacgtggaac
    12721 ccattgttga ggtgagaaac atcgccaaga cttacgacag cggcgtcgaa gcgctccggg
    12781 acgttacttt ggcgcttccc cgcggcgagc tcagcacgtt tttggggccg agcggttgcg
    12841 gcaaaacaac gctcctcaag atcattgccg gcctgatttc gccgagtgaa ggcgaagtct
    12901 gggtgaaggg aaacaaagtg agcggccccg gtcccgagcg cgccttcgtg tttcaagact
    12961 tcgcgctgct tccctgggca agcgtgttgc gcaatgtggc gttcggactg gagctgcgcg
    13021 gcgtgcccag ggacgagcgc gaggaaattg cccgcaagta tattaccgaa gtcggattgt
    13081 cgagcttcga atcccgctat ccgcatcaac tctccggagg gatgaaacag cgggtcgggc
    13141 tcgcccgggc gctcgccatc gatgccgaca tcgtgctcat ggacgagcct ttctcctctg
    13201 tcgatgagca gacgcggcgc aaatttcagg aagaattgct cgacctgctc aaaatcaatc
    13261 agaaaactgt gatcttcgtc acccatagca tcgaggaagc cgcctatctt tccgatcaga
    13321 tcatcttgtt gtcgcagcgg cccgggagtg tcttgaagat tatccggccg aacatcgaac
    13381 gcggcgagag tgccgacgaa atcagacgag acaagggcta tctcgatacc gtggacgaga
    13441 tatggaaaat cttgagggac tatgtatagc ctttggcttc actcacggct gctgacatga
    13501 ttttcttcgg caaaagaatt cctctttttt tctctctgct cgtctggttt ttcctgtggg
    13561 aacttgtcgg tcgggccgag tattcgatca tcatcccgcc tgtctcgcga gtgatcgtcg
    13621 cgggtttcac gatcctaccg acggagaagt tcgccgccgc ggcatcgatt tcattgcgct
    13681 cttttgctct cggtatggca ctcgcgatcg ccattggtct acctgtcggc attttgatgg
    13741 cgcgcctgga gaacctcggc aaagttctta gtttatgggt taacatcttt gtcagcgccc
    13801 cgatctccgc cttggtgccg attctgatgt cagtgatagg aattggcgag accaccgtga
    13861 tcgtcacggt ttttctcttc gccgtgtttg tcatcatcct ggatacgcaa gtgggaataa
    13921 agcaggcgga caaatcgctg gtggaaatgg ctttctcttt cggggcgcgg cgcggccaga
    13981 tctacacgaa agttttactt ctgagcgcct taccggagat tctagccggt ctgcgcctgg
    14041 gcgcgattcg cggtgttaag ggtgtcgtca tcggccagct gctgattgcg atccttggcg
    14101 tcggcgagct cttcgagctt tactcgcgta actttctgat ggaagaattc tgggcgctgg
    14161 tcatcgtcgt ttttctgttc gcgtttgcgg tgtccgaagc tatcgctttt ttcgaacgcc
    14221 gggttgaata ttacgcaagc gctcgttaac aatctcaccg cggcgaaacc cccgtttatt
    14281 ttgccaaaaa aatctggcgc atctcctcac ggagcttctt aaactcctcg gcgctaggct
    14341 cttccataaa cagcgtgcgt tgcgccacgt ctgaggcgcc tggaaaagcc ggggaaattt
    14401 gcggcgccag cacaaattca ccggttgcgg ccatggcttt ttgcccttcg ggcgagcaaa
    14461 tgtactcgac gtacaatctg ccggcattcg gatgcgcggc tttcctgctg actcccatgt
    14521 aattggtatc ggcgagatat ttgttcaagc gcacataatc gatgggtcct ttatattgtc
    14581 cgacatattt caagtaggtt atgcccaagt tagcttcacc cttgataact gtgttgacga
    14641 tcggcgcgaa cgattccacc ataagcggtt gctgctgcga caggcccttt gcgaactctc
    14701 gccagttgat accctcgtaa cgatccaagt cggccaggaa ctgcgccgtc gtggtatggc
    14761 gcgaaggatc cgggatggtg atttttcctt tccagcgcgg cagcaggaga tcgctaaggg
    14821 tcttgggcaa ttcttctttt ctgaccaatt cagtgttgta aagaatgctg atcggattaa
    14881 accgccatgg cgtcaagatg ccgtccgctt cgacaaattg ctgcgggaat tttgttgcgg
    14941 acggcgggat gaatttgtcg aagactcctt ccgacttgag aatcagctgc ggtcctttgt
    15001 tgccttcgat gacatcagcg cccgcctgac cgacacgcca ctcgttgagc gccctttcca
    15061 ggatgccggt ggtcgaagcg cgccaatact ccactctgat gccgtacttc ttttcgaaag
    15121 gatcgttgat taccttcatg gtctgcggcg gcaccgcggc gtagaccacc actctggctt
    15181 ctttcttggc cgcatcgatg tctatcgttt gagcaaaaag cggcatgaac caggccatcg
    15241 taacgaggaa aaatattaga aggaaaaaac ttttgcactg cgtcatttcg acacccccgg
    15301 gtaaagatct gagcgcaaac tattagcgcc agtgctggtt gtcaacgcga tggccgtata
    15361 gtaatggtcg cattgacgtt caaggagaaa tcgatgccac tgttgatccg cgccgaagat
    15421 gtaaacggct tattgaccat ggaagaagcc atcgacgcgg tggaagcggg atttcgcgag
    15481 tggggcaaaa gccctgaact caacgccccg cgaaagcgca tcatgactcc cgaaggcgtg
    15541 cgcgtcagcg ttcatcccgg gggaattcct tcgctcggag gcattggcgt tctggcgcat
    15601 tcggagcatg tcgccgtatc caaagaagtt cagacctatc acaacatggg cggaccggta
    15661 accgtcctgt tcgacgttaa ggacgcctcg ttgatggcga tcctggtggg ccgggtggcg
    15721 ctcaaggaac tgccggcaga aaggccaacg ccgataagga ctgcggctac cagcgcggtc
    15781 ggcacgcgct ctctggcgcg cgcggactct aaaattttgg gaattctcgg cgccggcggt
    15841 gaagcaaagt atcatctatt agctctcgcc aggacccgtc cgttcaagac ggcaaaggtt
    15901 tactgccgga cgcagtccac tcgcgagcaa ttttgccggt cgatggaaaa gctggtttcg
    15961 tgcgaagtcg cgccggcttc tagcgccaag gaagtggtcg agagcgctga tgtgcttctc
    16021 accgccacca actccaatgt cccggttttc agcggcgagt ggctgcaatc aggtgcgcac
    16081 gtcacttcga tcatgggcgg caatgtgggt ctggttcgcg ccggagttgc cgctaggccg
    16141 cgccgcgagc tcgatgacac gacgatcaga cgcagcgacg tgatcgtggt caactccagg
    16201 gagcaggcga tgcaagatga gcaggcagac ctgttcggtc cggttcaggc tggatttcta
    16261 agatgggaag atgtcggcga gctaggacac ttgttgaata gcgtcattcc gggtcgcacc
    16321 cgcgcaaacc agattacgct attcaaaaac aacgccggtc aaggagtcgc cgatgttgcc
    16381 gtcgcgagta agatttactc gctggcgcga agcaacggta tcggcatgga gttctagcag
    16441 gctgctgaaa agactcctat gtttttggga agatgaatta tgaaagatga aagctgaaaa
    16501 ttgaatttct gttttcagat ttcgctttca tccttgtaat cgcgcgtctc cctcagaggc
    16561 tttgagcggc ctggcggccg gagtttctgc aaaccgctgg catctattcg ccgagaacta
    16621 tgaacatcga ccttcactcc catttttttc ctatcgaggc gttacagaat cccggcaaat
    16681 acgagagccg agcgcctaaa atggcgctcg acaagggcaa gttgtccgtt acctcccaga
    16741 tcggatttcg tccggggctc ggaccaggcg catacgatcc atcggcgcgg atcgaggcgc
    16801 tgaaccagat gggcatcgat ctccaggcga tctcaccctc gccgatcctg ctattctatt
    16861 gggaagagcc gccggtcgcc gcgcattttt cgcgcaaaca gaacgaggcg attcaagaca
    16921 tcgttgagag gcacccggac cgcttcgtcg gattcggcag cgtgcctttg cagagcattg
    16981 ctgattcgat cgagattgcc agggaagcaa aggcgatggg acttaaaggg ttagaaatcg
    17041 gcaacgcagt gggcgacaaa cccctggacg atcctatttt cgagccattc ttcaatgccg
    17101 cgcaagaact ggacatgctc ttgttcgtgc atccactcga gggcgggctc gatgccgatg
    17161 atccgctatc gcccattctc ggcaacgtgc tgcaattcac tttccgcacg accatgatgg
    17221 tcgagcgaat gatcgtgaaa ggaatgttcg aaaagtatcc gaatcttcga ctttgccttt
    17281 cccacggagg tggcttactc gcgttcaata tctggcggct cgatcattcc tacagcttgc
    17341 ggcccgagtt aaagaagatc attcccgaca aaccttcagc atatttaaaa aagctgtact
    17401 tcgacaccat tgtccattcc accgccgcct tggaatacct ggttaaagtc gtcggcgcgg
    17461 atcgggtagt catcggcacc gactatccga tggggatggg cgatttcgaa gccgtggcaa
    17521 aaatcatgca atcggatttg ccggcggcgc agcgcgagct gatcctcggc gccaatgccg
    17581 cgcgagcgtt aagtatgtag gcgcgcgatg cccgcaccat cgccccatac tggcgatatc
    17641 tcggattggg caagtctgtt agaggaggtt tctgtgcaac tctcactgat atttcttctc
    17701 gcgttatttt tcgcagcatg ctctacgatg aaggcaagcc ctcccacggt ttccattctg
    17761 gtcgatctcg atccgggcag cgcggatcgt accgtgatcg tcgaaagcat tactgcggat
    17821 aagcagggcc ggctttatct gccggatcgg gtctctggaa atattctccg ggtagatccg
    17881 aaggcgccca aaccggaggt ggtaggccga atcgaatcgc gagaaatcaa gggcaagaag
    17941 gtcgatgcca acgcttccgg tgttgccttc aatcaacagg gcgatttgtt catagctgtc
    18001 ggaccgttta gtgaggttgt gcgcatcaga ggcacggagt tgaatccgca gaagcctgga
    18061 tccgcccaaa cttttgccac cgggacgccg ggggccaatg gcattgcttt tgatcgttcc
    18121 gaaaaccttt tcatttcggg cggcggcagt gggatcatct atcaagttgg cccggagggg
    18181 ggcgcggctc aagctgcggt tcaagttgaa aagcacgccc ggacacttcc cgacggcagc
    18241 cagcaggcga ctgtcgccaa tggtttggat tttgacggca atggcgtgct ccatgtcgcg
    18301 gataccgccc gcggcgcgat ttggaaagtc gtcatcggtc cagatggcaa aggcggcaaa
    18361 ccgagcctgc tcgcacagag tccattactc gaaggcgccg atggactggc cttcgacggt
    18421 cgcggcaatc tttgggtgac ggctaacgaa cgcaacgccc tggtgagcgt tacctcggcg
    18481 ggcgcggttc gggagtttag caaaaatggt agccaagggc cgcttgagtt tcccgcggca
    18541 atagtctttg tcggcgacac tgcctatatc gcaaattacg acacgccccg ccgtgataac
    18601 atggacacta acggcaagac ggcgcttgac ggtatcggcg cttcgattgc gcagatcacg
    18661 cgttagcgtg agctatcttt gagcatgagc taaaactcaa tacccataat gacaaggttc
    18721 cgaatttcac cagagtggaa caaaccgaaa cgccgggacc ctccctgttg gttcgtaatt
    18781 tagagaagtt cctgggcgag gagccgcagt gtcgctactc tccatagaag gcgtgcgtaa
    18841 ggagtatcag gttcgtggga agagacttgt tgcgctcgaa tccgtggatc ttgacgtccg
    18901 cgagggcgag ttcgtcacca tcgttgggcc gtcgggctgc ggcaagtcga ccctgttgaa
    18961 tctgatcgtc ggcctcctgc gctcgagcgg cggacggatt gtttttcgcg gcgcggccat
    19021 cgacgctatc tgcacgcaaa tcggttacgt cacccaaaaa gacaatctgt tcccctggcg
    19081 gacactcgtt gaaaacgtcg agatcgcgct cgaaatcaga ggcgttgccc gcgtgttgcg
    19141 gcggcgcgag gctgcggaat tgatcgagcg cgtcggcttg cgcggctttg aagatcacta
    19201 tccgcatgag ctttccggcg gcatgcgcca gcgcgccaac atcattcgga ccttgatcta
    19261 cgatccggaa ctgatcctca tggacgagcc tttcggaccg cttgatgccc agacccgcat
    19321 cgtccttcaa gatgagcttt taaagctctg gtcgacgact aaaaagacca ttatcttcat
    19381 cacccacgat ctaatcgaag cgatcaccct ggcggaccgg gtggtgctga tgagctcgcg
    19441 ccccggcaga atcaaggcga ttgagccagt taacatcgcg cgaccgagag atgtcttcaa
    19501 gattcatgag agcgagcagt ttcgcgccgt ttatgaaaat ttatggcgag aattgcgccc
    19561 tgaagtgagg ttggctgaag cttaaacagg agagaggtgc ttatgaaacg gctgatgaag
    19621 attatatgcg ctgcgacgct cgggtgcctc ggcacctcgc tttccgctga cgcgcaaacc
    19681 ccaagaaaag taaagatgac gattccggtc gtggcgcact cgatgacccc ggtttacatc
    19741 gcccaaagca agggcttctt cgccgaagag aaattggagc cggatattac ctctaccgga
    19801 ggcggcggtc ctgatatccg ggcgctgatt gccggggatg tcgagtttag ttttaccacc
    19861 ggcgacaacg tgatcctggc gcagcaagag ggcaaaagac tcttgatggt gatgagcgcg
    19921 ctcaacaaat tgttcatcaa ttgggcgatg cataaagaag ccgccaagag caagggcgtc
    19981 actgaatcga tgccgctggc ggatagaatg aaaagcctta agggtctcac catcggggtc
    20041 acgaatcccg gagctttgac cgcgcacctt gccgcctttg ttgtgcgcaa ggccggctat
    20101 aacccgcagc aggatgtcaa catcgtccct gtaggtgcgg gcccgacgtg gcttgccgcc
    20161 ctggaaaatc gcaaggttga cgtcgcatta acggcgccgc cggtgcccga gaccgcgatc
    20221 agccgcggct tcgcaattat gttcatcaac aacgccaaag gcgaggaccc gtcgattccc
    20281 gagttcctga tggaaaactt gatcgcgcgg cccgagaccg tcgccaaaga tcccgatctc
    20341 gtgcgccgca tggtgcgcgc cctcacccga gcaaaccagt gggcgcttaa gagcactccc
    20401 gagcaagtcg ccgatgctct gaaacccttc atggccaaga cgccacccga tctgttgctg
    20461 gcaggggctg ctgcggtgct gccgactttg agctcggatg gtcgcgtcag cgagcgaagc
    20521 tttcaaacca cccaggagat cctcgagcag gcggggatgc taaagaagcg cgtctcctac
    20581 tccgagctgg tgaccaatga gtttctcgcc aaatgatcag ccgcagccga ctctttgttt
    20641 accggctgat tttcggtctg ttcctgctcg ccgggtggga attgagctcc gggcgggtaa
    20701 ttgatccttt ttgggtatcg agcccatcca aggttttcgg ctatgtctac gaggtatttg
    20761 ccgatggatc gatcttcgcc catctgggca tcactctcta cgaaaccttt accggttttt
    20821 tcattggctc cattttcggc attggcctcg gttttgttct cggccgcagg gaaatcctcg
    20881 ccgacatctt agatccttac gtggtcgcct ttaacggcat cccgcgcatt gcgctggcgc
    20941 cgctattcat tatctggttc ggtatcggcc cgacttcaaa agttattctg gtcatcagcg
    21001 tggtgttctt tcttaccttc tttaacacct ttgccggtgt caaaggcgtc gatgccgagc
    21061 taaagaacgt cctgcgcatt atgggcgcca gcgagcgaca aattctaatg aaagttactc
    21121 tgcccgcgat cgttccttgg attaccaccg gcttaaagat cgcgcttcct tacgccatcg
    21181 tcgccgcggt ggtcggagaa ttcatcgccg ctagcaaggg gctgggttat ctgatcaatt
    21241 acaatacttc gctgttttca acgaccggtg cgctgggcgg aattcttatt ctcgcgttgg
    21301 tcgtcgtgct gtgtaacgag gggatcagcc gcgcggaagc ttatctgttg cgctggcgtc
    21361 ctagggaaga aaaaaagcgc gaaggcgaat tgtattgaag caagatccaa tgtttatcgc
    21421 gtggtttggc cgaccaaacc gaccgtagcg gcgacgccgg attcaatgcg ccatcctatt
    21481 gcttccccgc ctgtgacacc tgtcagttaa cgtcggcttc gcagagataa tcgtgagatc
    21541 gaaacaccgc attcagcgca aggaactacc agtcgctatt cggggcggtc tgtggatggc
    21601 cggcgccgcc gctgcattca cggtcatgac cacactgatc cgagaagcgg ccaaaggcgt
    21661 tcaccccttc gagatcgcct tctttcgcgc tctggttaat ctcgtcttga tgttaccgtt
    21721 tgcgctgcgc accggcagcg ccggcctcag aaccaacaat cataaagcct ttgcgctccg
    21781 tggtgtgtgc gggcttgcgt ttctgatgac gtacttcacc ggcgccgcgc tcatcccggt
    21841 cgccgagtcg caggcgctca ccttcacttc gccgctctgg ggcactctcc tcgccgtcac
    21901 ctttttgcac gagcggttga cattggctcg cgctttggcg gtcatcgtcg gatttgccgg
    21961 agtgcttgtc atcctgcgac cgggggccgt gcaggttagc ttgggcgcgg ttctcgtgct
    22021 cggtggcgcg ctcagtgccg ccggaagcaa caccattgta aaattcacga ctcgcacgga
    22081 tcacccggac accgtcgttt tctatcagat ggtctacgtc acgccattga tattcgttcc
    22141 ggcattatac atttggacct ggccggctct cgatcaactg ctgttgatga tcggtgtcgg
    22201 tttctttgcg actctgaacc agcgctttct ctcgcgcgct tacgcatccg cggatgcgat
    22261 cgctgttctg ccgtttgaat tcgctcgcct gccgtttgcc gcggtcatcg gttttctagc
    22321 ctttcgcgag cttcccgagg tttgggaatg gataggcggc gcagtgattt ttagcgcgtc
    22381 actttatatg gtccggcagg aagcacgcgc gacgcgctga aacgccgaag cgattcccgc
    22441 cagttaaacg ttcacaggtt tcaaatcaga accagccgga ttactaagta ttacgcggaa
    22501 gaaagattac cgagtgagtt atcgttggag caaatccaag gatggatgga ttggtcgagc
    22561 tgtggtgttt ctctcgcagg taggttgcgg cgcctcacag caagccggcg ggtcgcttgt
    22621 agtgatgctg gctgtcgatt ggtctttcaa ttcctcagct cttctccgga aagctctgca
    22681 cgcgacgacg gtcatggcca tggctgcgga tatggttagg ccagcgcctg aacagccgaa
    22741 tttaagaaac acgatgatca gatcgtccca cgcacgccgt cgattgaaga agttcaacat
    22801 gttcgttcct tcgcttcaaa ccggagaagt tctcttggcg gcgaaaactt ttagcaatgg
    22861 ctgtgcccaa cagttccaac atcaggtggt agacgcacga gaaaaagcgc aagattatcg
    22921 caagcttgcg atccatgaaa gagtgggttg gcgcgagctc aaaaccggac tgctcggcca
    22981 gagttagaaa ctcacaagtt tgtgcggtgg caacttcaga tgcgatcttg ttggttttgc
    23041 aggctggtga tggagtcgaa caacacagtc gaagcagact tgatcaaata gtcacaggaa
    23101 ctgcgtttgc agcacaaatg agccggatcg tgctcatgag ttacggcgaa agacccaaat
    23161 ctccacacga gggccgcgag aatcgataat aaaagcacgc gcagtggtgg gagccgttac
    23221 taaccgcgtt tgccgtagac cttgtcgata aatccttcct tctcgatttc gcgcacaatg
    23281 ctgtcgtcaa tcaaattgtc caccgtcatt ttgctgccgg gattttgctt caaagcgtcc
    23341 tcgagctgga tcttcattcc ctcgcgagtg gtgtagggaa ctcgctcgat cagtttggcg
    23401 gtggattcgt acgcactgtc gagatacgct ttgttggctc cgcgcaaata tttggcgatg
    23461 agcttttgcg ttgcgtcttt gttggttttg aaaaaatgtc ccgtttcgat cagcgccatc
    23521 acagcccttt ttacaaccgg gcggttcttg gctaggtaac gttttgtcgt tacggcgcag
    23581 ataaacggca acggataagg tttttccgaa tcggccatgt tcgccagaat ccggtgcggc
    23641 aggccgccgg ggatgagatg tacgttccct ggtggagagg ggaatccggc gatgattttg
    23701 cggctgaagc ctgcggctct ctctgaggcg ccgccgactt gaagaatttt cacgtcttcc
    23761 atcggcttta agcccaagcc tttgagaagt tctcgcgccg ccacgtcgga tacactgccg
    23821 aagcggctga taccgatgga tttccctttc agatcttcag ctgatttgat gttgtcgagg
    23881 acgaccagct catagggtag agtattgata aagcaggcga cgcttacaat gtcggcgccg
    23941 cgtactgctg cattggcaac cccgcttcct gcgccgttgc caatctgaac gtcctgagcg
    24001 acgatggccg atactgcaac ggttgtgctg ccgatataaa ccacctccgc gtccaatccg
    24061 tgttttttat aaaagccggc atcggcagcg atggtccaca atgagttcag ggcatccgtg
    24121 gagctgtacg caaccctgat gctttcctgc gaatgcgcat ttcctgtggc aagaccgatt
    24181 agcacgaggg caatggcaga aataatctga cgataaccgc gactgctgat cataagtcct
    24241 cccgttgacc gccgattagc tcgtttagaa cagtcgatgt ttggaagtgg atttctttct
    24301 tgtgagtcga tgaaagttcg cttccggagc atgggtcgcg ttcttctcga tcccgctacg
    24361 gatgaaccca agtagcgcgc cggttcatgt gccgcggccc cacaaaccgc aataacgcct
    24421 caaaatgaat ggcaatgctt gctcgtagcc gggcatactg gcgtatttcg gaagcttcat
    24481 ctccttctcc acggtatccc agcatttgcc ttcatgcgcg gccttcttta cttcggcgga
    24541 agtttcctgc aggaaagtaa gcatctcctg cgcgtcgttt tttgtgccga gccgaccgcc
    24601 cgcgccggga tgaccgggga tgagccgttc ccaatcgatt gccagcacct ttttgatcga
    24661 gtcctcccat tcgagcggat aggaatcgat catgccgcgt cccggcaggg agcctaccgg
    24721 cagccaatcg accgtaaaaa tgattttctc cgccggcagc cgcatgacca aagtcgagtc
    24781 ggaatgattc aggccaagat acgtgagctc cagtgtggtg ccgccgagtg taatcgtccg
    24841 atgagtatct actgtctcgt cgggaatcac cgtgtgcgga tccttgagtt gttcaagacg
    24901 ttctttggct cgcttatgtg cgatgaacgt ggcgccggcg tctttgaagg gtttaccacc
    24961 cgctatatga tcgaaatggt gatggctgta aatcacgtac ttgaccggct ggttggtgac
    25021 cctcttaatt tcttcgaggt aagtggtcgc cgcctgaggc cgtccatagc cgatcggatc
    25081 agtcgcaatc acgccctcct tggtgacgac aaacatagcc tgagcgccgc cgtaccgaaa
    25141 aacataaaca ttctcggtgc cgtctacctt agtagtggca aacgggggct gttgctgcgc
    25201 ttgggcggtt ccgtccaata cggttatcgc ggctgcgcat aggatgatct taagataaac
    25261 tgacatggtg cctccgaaac attgttcgag catgtacctg taattgctca ttttgcgatg
    25321 gagagtatct aaccgagtac ttctatgtca atgcatcgtg gctaacatca ttcgattctg
    25381 aacgacggta aaacagaaga ggctcttagc gggaatcaaa atctagtcca aatatacccg
    25441 gtcgagtcag tcccctgagt catcgcatat gcggcgcgag tttagtattt aaccgaagag
    25501 atcatcgagg gttatttccg aacctggtaa attgaaactg cggctgaatc aaaatcattc
    25561 gaccggctcg catggccgcg ttttattctt tgggttgtca ccagaagtag ttcggttttt
    25621 gatagaaaat gttggctcat gaaaactcag cgaaagaacc cgatatcctc ctcccacgcg
    25681 aaaatccagc aactgctcaa gaaaagcccc tcgccggcac atgcgttagc taatcttgga
    25741 cggctcattg agagtggcgg gagccagttg attgacaaga ttccatcaac cgaattgccg
    25801 ctactgaccc ggctcctcgg cagtagcgcc tatctgagcg aggttctgat cgggcgggga
    25861 gagaactggc cggagctatt tcttgagcag atcaaggtca agcgaaagag cgttgacgac
    25921 cacctaagcg acctcaaagc cgtcacgaca gcagcccaat cgcttgacga attctgcgcc
    25981 gggctccgcc gtcacaaaca acgagagtat ctgcgcatcg gcgcgcgcga tctcgacccc
    26041 tcagcgccgc tggaagaaac cgtcgaagaa ctgacatctc tggcggaagc atctctggaa
    26101 tcagcttatc tctactgccg gcgcgaaacg gaacgggaag tcggtcatct gaatttaccc
    26161 ggcgcagata agcccaacgg cttcgtcgtg cttggaatgg gaaagttagg cggcggtgag
    26221 ctgaacttca gttccgatgt cgacgtgatt tttctgttcg agaatgacga gggggagagt
    26281 tccggtggac caaagggcaa ggctgagccg agagcgttct tcgctgcgat cggcaaaaag
    26341 attattcaag cgatgggcaa agtcaccgaa gatgggtttg tctttcgcat cgacttgcgg
    26401 cttcggcctt tgggtgtcaa cggacccttg gtgcagtcgg tggactcggc catgctctat
    26461 tacgaatcct gggggcaatg ttgggagcgt gctgccttga tcaaggcgcg accggtggcc
    26521 ggagatagac acttgggcga tcgttttctg aaggaagtgg agccgttcgt ttatcgccga
    26581 tatctcgact acaccaccgt cgaagagctt cgccatatga agatgcggat cgaaaacgag
    26641 ctgttgagcc cacaggataa ggaaagaaat atcaagctcg gttatggcgg catacgcgag
    26701 atcgagttct tcactcaggc gctgcaactg gtcaacggag gctacgaacc ggctctgcga
    26761 catcagaaca ctttgcgggc gctttctgaa ctagccgcgc ataagttcat atccgccgat
    26821 gagatgaacc agttggccga ggcgtatcgc tttctgcgcc aggtggagca caaagtgcag
    26881 atggtgcaag aagcgcatgc gcactcgatt ccgcagggcg aagaagaaga aacagcgctg
    26941 gcgcggcgcc ttggatattt gccccaaccg aagcgcagcg agcgcgagca gttctggtcg
    27001 gcgaaaaata ttcacagtga aaacgtccgt cgtacattcg atcggctgtt ttacggggcg
    27061 caaaaagaga tcagtcatga cggccaatcc cacgccggca ccatttggca tgatctcgat
    27121 cagcgcgaag tgattatccg tgaactcgag aagctgggat tttccgatgc caacaaagcg
    27181 tacgaaaatc ttcttgcggt ccgcgacggc gagagcttcg ctccgccaag cgccaaacgg
    27241 ctgaaggtca tgcgtacgtt aggcccggcg ctgatcgcgg aaatcgccag atccggagcc
    27301 ccggaccaag ctcttctgaa cttgtcgaga ttcagccatc gaatcggcgg ccgcagcggc
    27361 tttctcaggc tgctggcgga aaatccggaa accatgcggc tgctgattac tctgtttgcc
    27421 gacagccaat tcctcaccga gctgttttta actcgcccgg agctcattga cactctaatt
    27481 cgcgtcgatc tgacgcgcat cgataagagc aaagaatcga tgatggcagg gctgcgcgag
    27541 gcggtcgctg agcaggacga tattgaaggt aaactgaacg cactccggcg ctcaaaaacc
    27601 gaagagttta tcaggatcgg gcttcacgac ctcggtggca gcatcgacct ggaaccggtt
    27661 ctacagcaac tttcggattt agccgatgct gtggtggagt gcgcgttgca attggctctc
    27721 gaagagctgc aagaaaaatt cggcatcatt cctcaaggcc gattcgccgt tctgggcatg
    27781 ggcaagatgg gcggccggga gctggattac aactccgact tggatctggt ttttgtttac
    27841 gccgctgatg aggacgcctc cagcagcggc ggcccgcaag gtaaactgcc ggcccatgat
    27901 ttctacgttc gagtcggcca aaaactgatc acctatctct ccgcgcccac ggaagaaggc
    27961 atcgcctaca agatcgatat gcagttgcgg ccatcgggta aggccggtcc cttggtcagc
    28021 tctctagaag cttttcggga gtaccatcgc gccggctcgc aactgtggga gcggcaatcg
    28081 ttgatcaagg cgcgctatgt tgccggtgac tcgagcttgg gaataactct cgagaaactc
    28141 gccgcggatg tcgcatatgg ctcaggtttg tcgagcgaag gtgtcggtga gattcatcat
    28201 ctcagaatgc gcatggaacg agagctcgcc ggtgaaagcg acagccgttt caatctgaag
    28261 aagggtcgcg gcggcattgt cgatatcgag tttcttacgc aaatgctcca gctggctcat
    28321 ggcattcacc atcccggtat taggtgtcag gcaactttaa aagctttaaa ggcgttgcat
    28381 cagcagggaa tcctgaagaa aaaggaatac gctacgctgg ctgacggtta cctgtttttg
    28441 cggcgcttgg atcaccgtct caggttggag cgcgatcaat ccatcgacgc ggttgaacgt
    28501 gacccaggcc gccttgacgg cattgctcga gctttgggct atgacagcgc agttactccc
    28561 agggctaaaa atctcgccaa ggccgggcgc aagttgctgg cggactatga gtcacggcgt
    28621 gagaagatcc gggcgtgcta cgagcggttt ttcgtgggcg cgaataacga gtagtgattg
    28681 tgctttaccc ccggataaaa catcaatcta caaaaaatct aaacactcta gctagccaat
    28741 gctcggtgaa acggcatcta tatgccgggc tttcgatctg cagtggcatc ggaatatttg
    28801 ggtttcatcg aaaatataga atttgattat tgacagtagc acgcgttgtg cgatatcgtc
    28861 acgttcatga aaacacagat ttacaagggc cttgccctag cttcctttgt cgtagtcatg
    28921 tcagctccgc ttcatgcgca gactacggct ccggcggatc aatcccccga acaacccgtc
    28981 actgcggaat tggggtacgg agtcgcctcg gcgcttgcca gcgtcttcta catacctgcc
    29041 aaagttacct atgccggcct gggtctggtg accggtggac ttgggtatgt gctgacgggt
    29101 ggaagatccg atgtggcgag caacatcatc tatccggccg ttcgcgggca ctatgtggta
    29161 acgcccaatc agataaaagg catcgatccg atttacttcg tcggagcgcc gccggaatct
    29221 ccggagccgg agagctacgc tgccggtact cccgcgacgc gctaagcccc gaagcagacc
    29281 cctcgtttcg acgacctgat aaactccacg agggtcatga catcgtcatc ggccctcatg
    29341 ctttcctcta cctctctgag cgcaactgaa aggttgcgtc cttttcggaa tagcacgcga
    29401 aaagcgttgc gtatggctcg aatccggcga tcgtcaaagc cggcgcgctt caatcccacc
    29461 acattgacac cgcgcaccgt gtgctgccaa tcgataatcg cgaaaggcgg aacatcgcgg
    29521 ctggtgccgc tcaagccgcg catcatggcg tgctcgccaa ttcggataaa ctggtgcacc
    29581 acgcagttgc cggagatgaa cacgctgttg ccgacgtcga catagccgcc gagcaaagcg
    29641 ccgttagcca tcacgatatg gtcgcctaac ttgcaattat gtcccacatg cgacgtggcc
    29701 atgagatagt tgtggttgcc gatgattgtt gatgagttcg gtgccgtgcc gcgatgcact
    29761 tgagcgtgtt cacggatgac gttgtggctg ccaatggtta aataactctc ctggccctgg
    29821 taagccttat cctggggcgc gccaccgaga attgcgccgg gatgaatctc gttattctcg
    29881 ccgatctcag tccatcccgt gagataagca tgagccatga tccgcgttcc gcgtctaatc
    29941 ctcaccggcc cctcgatgac cacatagggg ccgatctcta cttccgaatc gacttccgcc
    30001 cgggagtcga ccacagctgt cgaatggatg ctcatgagaa tctgcgcgac tatcgattct
    30061 ccttttcgtt ttcttcgtct tcgccgagct gcgtttcaac tttgcgtccg ggaatacgat
    30121 atctctcctc ggtccaggcg ccgagatcga tcagctgaca acgatccgag caaaacggcc
    30181 ggtgtggatt atcgtcccaa gagcattctt tgcggcaggt ggggcatttc acatgcagag
    30241 gcatggcttt ctcctcagct attcaacgta ctgctggcaa acaactcgtc caccgaaagc
    30301 tttttggcca acaaaccttg gtctttggaa taatcgatga accgctcgag attagcccga
    30361 ttcttttcta agccgtgggg ccatggatca tcgccgaaga tttttctctc ttcttcgaaa
    30421 agatgcctgc cccagacgaa ccgtgaccaa ttaggatcgt cgtagtatgc cttgcaggcc
    30481 attttagctt ggtcgaacgc ggtcatcacg cttacggcaa gagcgggatc tctttttaga
    30541 acgtcatcct taaaagcgac aacgtgcatg atcgggtagt agccgttttt ttggaagtac
    30601 ttgatctctt cgcgcttcgg atctggaaat aaacggcgga tcttcttcga cccttctagc
    30661 gcctcggctg gcgggtgtgg aaccatgagc gcgtcgatct cgccatgctc aagcaggacg
    30721 ccgatctttt tccccggcgc aagcagctcg atatgcaccc cagcctcggg cttgaactcc
    30781 accgcctcgg ctttggagac cacccattcg atcttgcgcc agggaacatt gtattcagat
    30841 tgcagatcac cctttgccag cacggacaag gtcgtttgaa atgtactgag gcccactcgc
    30901 ttaccaggca aatcgtcggg cgaggtgatt tcggcgtcaa cattcaccca catctgcgat
    30961 aggctgaaca aacggcgtgg aaaaaccggg attgcggtga atggcaagcc gcgacttttc
    31021 gccatcagat aggaggagag cgagagctca cagatatcga actcttgctt ctgcaacatg
    31081 cgctcatgac ggtcttgacc atgcttgaga ggatgggatt gaccgacttc gagcacggtt
    31141 acgtttgctc cctcgacctg aatcgatcca tcgaacaggg gaatatgacg gtcgtagtgg
    31201 gatagcgcga cggtcagatc tgctttagcc atactcgaat actctcctcg tccaatcgat
    31261 tctttgctta ctcactttcc tggatcttgt ccacatggaa cgatgaacgc cgcagcagca
    31321 catcgctgag aacatcgcac agatgacagg cagacatgct caagatggcg acggagaggc
    31381 ctgcggcgct aaaaatcggc gaggcggcag agatcacatt tgcaaaaatt aggactattt
    31441 tgtggatcta acaactcacg gttctcggct agatgcatga tcgcctttga agatcaaaaa
    31501 atcgcaacga aaatttaggt tgcgacgtca gtggttccgc caccggtttt cagccgatcg
    31561 agcaaagccg taatttctga caaacatgag cgcgcttctc tctcggcctt ctggatctct
    31621 tcggcgggcg cgccgctgct cactgcatct tcgtactggg tcacggcaac gccgaaggtt
    31681 ctaagtaatc ggcgcgtttc gtcgctgggc atgtcctctc ctttgtttaa ttccaggatg
    31741 gcgtcaatgc gaaatcttga gctcgcgcat tttgctctcg ggaataaaac cccgatcatt
    31801 ccagccccgg gccaggtaat agctttggat catctcatgc agctccgcgg gccgtagttc
    31861 cgtcccttga gccactccgt cggctagttt ttcatcgagc aatctgggtg gaagccaatc
    31921 atcctccggc tgccagcctt cgcgaaggtt gaacaatttt ttttgtgcgt ttatccgttc
    31981 accgatttgt ctcaactcgg cactcgagta gggccagccg gtcactttgg cgagtagatc
    32041 ggcagcttct gcatagaaat ccgtgaagca tttgcgcaga aatttgcaaa cgatcaatga
    32101 atccagcacc gcggcaaaat cttcggaagc ggcgaccagc aatccgcgcc cggcatcgcc
    32161 acgcagacgg tcgacttccc cggagaaatc gaactcataa gcgccagagc ggttgtgaca
    32221 agcgccacgc gggctgaccg cgagccccag tgccatggtt ttcaagctgc gaggttcgta
    32281 tcccggtaac tccagacctt taacgtgcat cgcccattgc cccgtatctt tgccgacgat
    32341 ggcagccgct cgtttcgagc cttgtgccag cagatcgcca aggcctctgc gctcggcgat
    32401 catcggaata atcttcaaaa atgcgtcgtc ccggccaaag cgcagcccta aatgggtagc
    32461 ttccggcaac agccccttct cagcgcattc catcgcccac gcgatggtcg cgccggtgct
    32521 gatcgaatcc agaccataat cgtcgcaaag tcgcgcggct tgaagcaggg tgtgcgggtc
    32581 ctcgatctca cacattggcc ccaaggcgaa gagtgtctcg tactccaggc gctggtcctg
    32641 cccgtccaag gatttgaaaa gacgttcgca gcggatggta caggcggcac agccgtttct
    32701 ccggctgaaa ttattttcgg tgagactttc gccgctcaag ttctccgcgt gcgcgaacgt
    32761 ggagtgctga aaattgcgcg tgggcagact gccgagacgg ttgaacaccg caaggttggc
    32821 gacggttcca atctgacgat acttagacgt tactgtcccg acgctcttgt gtcgcatcgc
    32881 cagcgcgagc tcggcgacgc ctctgccatc ggcgatggcg gtatccatat cgccgcgaat
    32941 cgcaatcgcc ttcaagcgct ttgcgcccat gacggcgccg accccgccgc gcccggcatg
    33001 gcgtccatca ttgctgatag ttgcgaacct gacgccattc tcgccggcta aaccgatggc
    33061 ggccacgcga aaattgtcct tgttagactc ggcacgaaga aaagaatctg tctctacggc
    33121 gctcttgccg accaggtgag tagcgtctcc gaaggaaaca cgaccgcgat cgatcgacag
    33181 atacgtccaa gccggtgatc gaccggtgat caccagggcg tcgactccca gtctctttag
    33241 ttccaaggca aagtaactgg aagagaggga gtcggcgata aagctggtga gtggtgactt
    33301 cgtgacaacg gcgtatttag cagtggtggt cagtggcgtg ccgaccaagg gcgcgctggc
    33361 aaagatcagt ggattttccg gcgccagcgc atcgattcca ggcggagcaa attgatacag
    33421 gagatcagcg ccaagtccga tgccgccgag gctacgacgc aggcgcgagc tgtcaatctc
    33481 gagcgaggta taggatgccg tggtcaaatc gattttgagg atgcggccat gaaacccatg
    33541 catagatcta cctgcgccgg atgacctgat tatgtaatgt gatcacgaga ttagctaaac
    33601 acaagccgga taccaggcgc aagccacagt agcaactctg aagcaagtat tctcaagccg
    33661 tctcgcgatt gctacagtga cgaggcttgt tgagtcagat ccgggactga actgttcaaa
    33721 gttcataatt acatactggc ataagcgttg ctcccgactc cgtgcgtaca agcagtgcgc
    33781 gacgaaatcc ttcgcccaaa ggagttctac atggaactct cccgacgcgg ttttctgcgt
    33841 tactcagttg cagccggcgg tgcgaccgct ctaagcggat tgattggctc tggtgtaaat
    33901 ctggggccgg ccatcgcgcg ggcacaggag ttgaggatca aaggcgccaa agcaactcca
    33961 agtatttgcc cctattgctc tgtcggctgc gctactctgg tgcacacgat cgatggcaag
    34021 atcgtcaaca tcgaaggtga cgcacgcagc ccgtacagtg aaggaacgct gtgccccaaa
    34081 ggcgcggcca tctttcagtt gcacgtcaat cccaaccgac caactcaggt tcttcatcgc
    34141 gcacccggtg ccagcgactg ggaagtatgg gatctcgagc gggcaatgga ccgggtggcc
    34201 gaactggtta aaaaaacccg cgacgagacc ttcatcgaaa agctggaaaa tggcaagttg
    34261 gtaaattgca ccacttcaat tttctctctc ggtggagcca cactggacaa tgagtggaac
    34321 cacatccagc aaaagttgat gcgcgggctc ggcatcgtcc ccatcgagaa tcaagcccgg
    34381 atatgacata gctcaagcgt ccccggtctg gggacccgct ttggtcgcgg cgccgcgact
    34441 ataccttacc gcacaatggc ggagtccgac tgctgcgtgt tcatgggctc aaacatggcc
    34501 gaaaatcatc cggtcgcatt tcgctggccg atgaaggcca aagctaatgg cgccaagctg
    34561 attcatgtcg atccgcgctt tacgcgcacc agcgcgatgt gcgatatcta cgcgccgatt
    34621 cgcgccggct ccgacatcgc ttttctcggc ggcctggtca attacgtcat taacaatgag
    34681 cgttggaaca aggagcagtt cttccaggat tttctggtgc attatactaa tgcgccgatg
    34741 ctcataagcg acgaatttca ggacaccgaa gatttagacg gcgtgttttc cggcctcatg
    34801 cagtataagg aaaagctcaa cgaatggccc tacaacgggt ttctcggcga gtacaacaac
    34861 cagagctggc agtatgccgg cacccaggct gaaacacagg gttggacagg cgataccgcg
    34921 cgcagcggcg agcaacaagt cggcaaggaa caagcgccgc ccggcggaaa ccaagcggcc
    34981 ggggctaaag acggtgaagc tcaaaaggcc gcagccaagg gcccgccgtt cgagccgctc
    35041 gtccgcgcct tgctcaaacc tccgccgcga agagacgaaa cgctgaagaa cccgcgttgc
    35101 gtgtttcaga tcgtcaagcg ccatttcagc cgctacacac cggaaatggt cgaaagggtt
    35161 accggctgtc cacaggcgac attcctcaaa gtcgccgaaa cgattctggc aaactccggc
    35221 ccggatcgga cgacctcatt cgcctacgca gtggcgtgga cgcagcatac caacggtccc
    35281 caggtaatcg gctgctgcgc tctgctacag ctattgctgg gcaacatcgg ccggccgggc
    35341 gccggcatca tggccctgcg cgggcacgcc tcgattcaag gatccaccga cgttccgact
    35401 ctatatcatt caatccacgg ctatatgtcg caccctactg cacttaagaa gcacgacaca
    35461 ttgggtgatt atttggtcgc ggagaccaag acccgaagct actgggccaa cctgcccaag
    35521 ttcatggtgt cttacatgaa atcgatgtac ggcgatgcgg ccacggcgga gaacgggttc
    35581 ggctatgact ggcaccctaa gatcatcggc gatcattccc atatggccat gtttgccgcc
    35641 atggccgaag gcaaagtgag aggcatgctg ctgatcggtc aaaacccggc cacttcgctc
    35701 aacgccaagc tcgagcgcgc cggcatgcgc cggctcgagt ggctggtggt caaagataat
    35761 tgggtccatg agagcgcaac tttttggaag aatgctccag aaataaagaa cggcgaagtt
    35821 aatttgacgg acataaaaac cgaagttttt ttcttcccgt ccacccaagt cgcggaatat
    35881 gagggctcgt ttacgaacac gtttcgcatg ttgcaatggc actacaaggc agccgatccg
    35941 cccggggact gtcgcagcga tctatggttt actcaccagc tcgccaagcg gctaaagaaa
    36001 ctttacggcg aaagtaatga acctcgcgat gcgggattca agaacttgac gtgggatttc
    36061 gatccagatg aaaggagcga gggtctgcgt gcgcagaccg ctccggcaca ggcggaaggc
    36121 ggcgcggtgg gtggggcgac aaccggtggc acagccccgt cagccgctgg ctccagcggc
    36181 gctgcgcccg gggcggcgaa ggatcagcct ccaggaagcg cgcctgcgcc cggaacgccg
    36241 cagcacaatc ccggtgaacc agatggactc aagatacttc gcgagatcaa tgggtattac
    36301 accggcgacc ccaacaagca tgtcgccggg tttgcggatt tgaaagacga cggctccacc
    36361 acctgcgcgt catggatata ctccggcgtc tttccgaaac cgggagaaaa tcgttcggct
    36421 aaccggaagc ccgatccgcc ggatgcgccg ggcggcgcgc agctaggttg gggctactcc
    36481 tggcctgcca accggcgcat catgtacaac cgcgcgtctg cggacctgca gggaaagccc
    36541 tggagcgagc gcaaaaaata tatctggtgg gatggtcaaa agtggacggg ctacgatgtg
    36601 ccggatttcg ctgcgacaaa gccgccgacg gcaaaggcgc agcccatggg cattggactg
    36661 gacgctcagg atggaacgga cccatttatc atgctcgacg gcggtgtcgg ttggctctat
    36721 gtccccaccg ggttggtaga tgggccgcta ccgactcatt atgagccggc cgagtcgccg
    36781 gttcagaatc ctctctataa gcagcagtcc agtcctgtat tgaaatactg gaaaacatcg
    36841 ggaaatcctt tggctccggc gagcgacgca aagttcccgc acgtcatcac gacttatcgt
    36901 ttgaccgagc actatttggc gggtgccatg agccgctgga atccctgg
//
LOCUS       DASUVS010000333         3425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1888446, whole genome shotgun sequence.
ACCESSION   DASUVS010000333 DASUVS010000000
VERSION     DASUVS010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1888446"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2055..>3425
                     /gene="treZ"
                     /locus_tag="VF182_17870"
     CDS             2055..>3425
                     /gene="treZ"
                     /locus_tag="VF182_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020562666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX6439006.1"
                     /translation="MKAATLIPEIYNRRFPIGAEPAGGGVCFRVWAPQAKRVELLIGA
                     IESSEPDELKPEGQGYFSGFVSGCSAGDLYRFRLDGQEQLLPDPASRFQPDGPFGPSM
                     IVDASQFDWHDAHWNGVTLERQVIYEMHIGTFTLEGTWQAAGRQLPELKNLGVTVLEI
                     MPINDFCGNFGWGYDGVNFFAPTRLYGAPDDLRRFVDQAHGAGLAVILDVVYNHAGPT
                     GNFLKDFSNDYFTDRYENEWGEAINFDGPNSAPVREFFLSNARYWISEFHLDGLRLDA
                     TQALFDASEKHILSELVETVRQSAAPKSIIVIGENEPQDSKIMRPATDQGYGLDGLWN
                     DDFHHTAMVALTGHNEAYYTDYLGNPQDFISSAKRGYLYQGQYYRWQKKRRGAPTTEI
                     KPAAFVNYLQNHDQVANSGRGWRVHRMTDPGVYRALTALLLLSPQTPMLFQGQEFAAS
                     TPFAYFA"
BASE COUNT          807 a          865 c          895 g          858 t
ORIGIN      
        1 cgtgaaaaag gttacgaccc ggcgtggaac tagtgcctga gtcgggatat atctaccaat
       61 cgccacaact tccgacccag gcggtcccgt gcggctttac acccaatggt tcaggtcaga
      121 aatttcgcgc caataaagtg atcggatgtt tcgaaaatga tacgaaaatg ccagcaaggt
      181 accttccacc catgactagg agagcataaa tatgtcaagg ggaaaaatgt ggataagtca
      241 cgggtcacgc atttttttga aataatccgc ttggaaaata cttgacaact gtagccaact
      301 ctctatttga gttgaaaata gcaaattttt gagaagatca gtggaaccgc tgaatttcac
      361 atagaatttg ttgaccaatt cttcggcagt ttgctgaaag ctcgctctgt acggcatctc
      421 ctacacacag cgccaaatta tccacaaagt tatccacagt ttcggtggaa aattggcggg
      481 cagcctgcag attctggggg aaaatcatcg ccctgtcgca cactcgggat tggctcgaat
      541 gattgtcatg cccccactat ggccgcgcta tcagcctttt cgcagcaatt ttggttcaag
      601 cgccgcatca atcaatcggt gcttcaattc ttccggtgtg gccaaccggg cggattttga
      661 tcgcgaggta tcgtcacctt ggttcgactc aggaggtgac ttggacgtcg atgttcaatt
      721 gctccgttca gactatccaa actagtgtgg ctcgtaaggc gcggtttggc aaaaccgaag
      781 tagctcttgc gaatccggtg atcgtatttg ctgcggacga ggagtcgggc ccggttcgaa
      841 aagaatattt cgccagaact attctctggt atctcagccg cacgatatgg ccgcgagaca
      901 gagatttacc ggcaagctag tcgcggctag acggagctgg acacggcgga catcggatcg
      961 tttcccgtca gttctggttc gtcgtcggtg gcttgtaagt cctcagtttc aaggccgagg
     1021 ttttttgctt ccaggttaca agcgtagcat acgatcatgt ccagtaagat taacccttgg
     1081 tcatgggtcc taacccgata ggctttcttg ttgaggagaa tgccctcgca atgatcgcac
     1141 cttgaattcg gctccacccc tgcagagtga agcacctccg ggtgctccca atttctgaca
     1201 agagctgaaa gctcacgaga tgccggcaca gattctttta tctgtggcga tccgttcagc
     1261 aggtcttgcg ctcgctgcgc cgcgttcaga ttgcggtgag ttgccttgaa gcggagaaaa
     1321 ctttggtcga tgagacgacg cgaaatttcg acggagcttt ttgtccttga tttgatgtca
     1381 tcgagtcggg cgaacagttg gtcccggtcg attgccttcc tgcagatcac aatgccttcc
     1441 tcccgtgaag taattcgcct ttgcgcagca ataaacggac ccagccgtaa gataacggat
     1501 tttccgagaa ttaaggcgag agccaagccg aagatggaaa attggctgac agaaatgtac
     1561 aacccggaca aagaatgcac gcggcgcgag tgctgggttg tttaatcgcc gttcgatcct
     1621 cttcagatag aagcagaaga caaaggtgaa tagtcaaacg cgcggaaacg atttttaagc
     1681 gagttgaaaa taagttggga tacctctttc ctctcaccac cagtaagtct tgaccaggct
     1741 tggtgttcaa tcgataagca actcggcaga agcaatgcgc ttattcgctg gacaaatact
     1801 tctctggcgt gctcaatgtc atagagccgt tcttcgaatg tgcacggttc tccttggacc
     1861 taattgaaaa ctctttttgc aaaaatccat ccatttccat atgcgttaag aaccgtcaag
     1921 tttaaattgt gcaagcggtg ctgcttcctg gctttgtcta ttccggccaa gaacttttgc
     1981 gaccacccgg gttttaatgt tatgcgacct ttcctagaat gaaactgtag ttaaaggcaa
     2041 gatgtaggga ttggatgaaa gccgcaacgt tgattccaga aatttataac cggcgatttc
     2101 cgattggcgc cgaaccggca ggtggtggag tttgttttcg cgtgtgggcg ccgcaagcga
     2161 aacgagtcga gctcttgatc ggcgccattg agtcgtcaga gccggacgaa ttaaagcccg
     2221 aagggcaggg gtacttttcc ggctttgtat ccgggtgctc cgcgggcgat ctttaccggt
     2281 tccggctgga cggacaagag cagttgttgc cggatccagc atcgcggttt cagccagacg
     2341 gtcccttcgg tccgtccatg atcgtagatg cgtctcaatt tgactggcac gatgctcatt
     2401 ggaatggagt gacgctcgag cgtcaggtta tctatgagat gcacatcggt acttttactt
     2461 tggagggcac ttggcaagct gccggccggc agttgcctga attaaagaac ctcggtgtga
     2521 ccgtgcttga gatcatgccg atcaatgatt tctgcggcaa cttcggctgg ggctacgatg
     2581 gggtcaattt ttttgccccg accaggcttt atggcgctcc cgatgattta cgccggtttg
     2641 tcgatcaagc gcatggcgcc ggtctcgcgg taatccttga cgtggtctac aaccatgccg
     2701 gtccaaccgg aaatttcttg aaagatttct ccaacgatta ctttaccgat cgctacgaaa
     2761 acgaatgggg tgaagcgatt aacttcgacg gccccaactc ggctccggtg cgggagttct
     2821 ttctcagcaa tgcgcgttac tggatttctg agtttcactt ggacggcctg cgcttagatg
     2881 ccactcaggc actgttcgat gcctctgaaa aacacatcct gagcgagctg gtagaaaccg
     2941 tgcgccagtc agccgcgcca aaatcaatca ttgtcatcgg tgaaaacgaa ccgcaagaca
     3001 gcaagatcat gcgtcccgct accgaccagg gttacggtct cgatggcttg tggaacgatg
     3061 actttcatca taccgcgatg gtggctttga cgggacacaa tgaagcgtac tacacagatt
     3121 atcttggcaa tccccaggat ttcatttcca gcgccaagcg cggctacctt tatcagggac
     3181 aatactaccg ctggcaaaaa aaacgccgcg gcgccccgac cacggagatt aagccagcgg
     3241 catttgtcaa ttatctccag aatcacgatc aggttgccaa tagtggccgg ggctggcgcg
     3301 tccaccgcat gaccgacccc ggcgtctacc gcgcgctgac cgctttattg cttctctcac
     3361 cgcaaacgcc gatgttgttt caagggcagg agttcgccgc ttcaactccg tttgcgtatt
     3421 ttgcc
//
LOCUS       DASUVS010000334        15930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1451781, whole genome shotgun sequence.
ACCESSION   DASUVS010000334 DASUVS010000000
VERSION     DASUVS010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 15930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15930
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451781"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /gene="bioD"
                     /locus_tag="VF182_17875"
     CDS             <1..679
                     /gene="bioD"
                     /locus_tag="VF182_17875"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX6439007.1"
                     /translation="TFFACKLSALLRHSGYHVGVMKPAESGCAERQGKLFPEDAIRLK
                     EASGSQDSLDKICAYQLREPLAPSVAAQRQGITLKIDKLMDVYGEISSGHDITLVEGA
                     GGLMVPLLPSYTYADFARVLKLPMIVVAANRLGMINHLLLTLEHARCKGLSVLGYVLN
                     RLENGATLAAETNREALSTLTAVICAGELPHVAPAEDAHNGGADWFAEQFDLRILRSV
                     LCERSRQ"
     gene            complement(875..2035)
                     /gene="larC"
                     /locus_tag="VF182_17880"
     CDS             complement(875..2035)
                     /gene="larC"
                     /locus_tag="VF182_17880"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEX6439008.1"
                     /translation="MRIAYGDLIGGISGDMFVAALLDLGLPLELLTRELRKIATLEFA
                     LDAAKKTTHAIAATQFQVRPPSNEAPRSWEEIHQLIEASKLAPEVQETGLKIFQRLAQ
                     VEGKIHGVPTAAVHFHEIGATDSIVDILAAAIGFHELKIGALHFSKVPLGRGRAQSGH
                     GPLPVPGPATLELLKGIPVQWTELEGETVTPTGAAIMSVLGTSFGEEPSMTVETIGYG
                     TGQKEWPDRPNLFRLVLGASTDWRQEEMLVIETNIDDMNPQFFEHVMHRLFATGARDV
                     FFSPIQMKKNRPAVLVRVIAEPRNRDRLAKILFEETTTIGLRYHSVNRIILNRTEATV
                     KTRFGDVKVKIWQEPNGEKRISPEYEELKRIAIEKNIPLKVLRDEIVKSFKQ"
     gene            complement(2117..3328)
                     /locus_tag="VF182_17885"
     CDS             complement(2117..3328)
                     /locus_tag="VF182_17885"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX6439009.1"
                     /translation="MRIETERKMKRWREQRWLLDQVIQARGIDWDQGRTGKILRNCGP
                     GVQSDLQEVCRRIQKFVDIPREFARAASRREQQAYEAEGSGHLPEARDHFYIAACFYT
                     NAMWAVYEERNPKRMNWQEKKRACYDKFIRYAGRPIERIELPYQGKLIHALLHLPPER
                     KAGEKVPCVMYIPGMDGVKEDNPATGDPFLERGIAVLAIDGPGQGETREGGLTCSASN
                     YAEAGKLACDYLVKRSEIDPNRLGILASSMGSYWAPRVVAEEKRFKACAVSGVCMEPG
                     QYAIFNMSSPTFKLNYMYMSGYEDEAAFDEFAKTLTLKGVTAKISCPYMVVAGEDDEH
                     CDMKFVYELIGEIPAPKLLYIFEGERHSIRNPRSRSLLINWLVDRLQDKPFKSEKIFV
                     ETSGKEIHSDW"
     gene            complement(3408..4373)
                     /locus_tag="VF182_17890"
     CDS             complement(3408..4373)
                     /locus_tag="VF182_17890"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439010.1"
                     /translation="MNPTENCRIIVVVLVAFLAVGSGESRGQTKPRPVRVAYSALSAG
                     IGVLWLTHEQGIFRKYGLETNLVYMRSGTTAAQALLAGEIQFGHLSPAPMMTAWVQGA
                     DIAWVGTTIHQMVFTLITDATITQAAHLKGKKLAITRIGSASDLALRTALEHLGLSIK
                     DVAVISMGGIPDILAGMRAGAVSGGILSPPTSTAARDLGYRPLVHIPDLGKEFTFSGI
                     AARRTSVQAEPNIVRDFMAALTDGARIYKEDSGAAVRVLRKYMRAEDRILEQGYKEYD
                     AAISSPPYPGLTALEAVRDSLVDSAPQLKQIDLKMFIDDRFVKPR"
     gene            4583..4969
                     /locus_tag="VF182_17895"
     CDS             4583..4969
                     /locus_tag="VF182_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011357492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX6439011.1"
                     /translation="MLFDFANVLVFTALGLGFVGVNLLIGRLLRPANPQARKLSTYEC
                     GEPSTGSAWVNFNIRFYLVALIFIIFEVEIAFIFPVAVVFRNLVESGEGLFAFIKIFI
                     FLGILFLGLIYSWVKGDLDWIKKVRV"
     gene            4991..5503
                     /gene="nuoB"
                     /locus_tag="VF182_17900"
     CDS             4991..5503
                     /gene="nuoB"
                     /locus_tag="VF182_17900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX6439012.1"
                     /translation="MKPLINSLPETVFTTKVDDLLNWGRASSQWYMLFGLACCAIELM
                     QTGGPRADLDRFGAVPRATPRQSDLMIVAGTLTYKMAKRTKLLYDQMPDPKYVISMGS
                     CSNCGGLFQLAYSVCKGVDKIVPVDVYVPGCPPRPEALTEGLLRIQDKMMRERWLVKS
                     NSEPESAVQA"
     gene            5557..6012
                     /locus_tag="VF182_17905"
     CDS             5557..6012
                     /locus_tag="VF182_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX6439013.1"
                     /translation="MSGFKADVPEPYLFVNAEAIVEVCRFLHDDADLKFEVLSDQTAV
                     DWPKEEKIQLVYHLYSYSDRHQVVLKVDLPRDTPKIATVENIWKVANWFEREIFDLFG
                     VIFEGHSDLRRILLPEDWEGFPLRKDYIEQEEYDGISTQRAPLVEKLLR"
     gene            6021..7163
                     /locus_tag="VF182_17910"
     CDS             6021..7163
                     /locus_tag="VF182_17910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052506.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX6439014.1"
                     /translation="MNEIVQDQHAGFYTEDMTISVGPQHPSTHGVLRFVVKTDGEVIG
                     EAIPDVGYLHRSIEKIGEKVTWHGYVPYTDRADYLAAMFANQVFCMAAEKLSGTPVTR
                     RGEFCRVIACELNRLASHLISVGTFGQDIGAMTPFVHALRERETINNLMEEICGARLT
                     YNYIRIGGVGYDILPETCARIAEFLDHFEPIVHEFNRLLSENKIFIERLAGVSVVSKE
                     DALQYNLVGPNLRGSGVKWDIRRDIPYSVYPELDFDIPVGTGHIGTLGDSFDRYWVRI
                     RELQESCKIIRQCLRMLPGGPAISKVPRKFKPPAGECYVRVEAPRGDMGFYVISDGSE
                     YPYRVRIRTGSYTAMSIIDKLSKGIMLADLIVLIGSLDVDAPEIDR"
     gene            7135..8376
                     /gene="nuoH"
                     /locus_tag="VF182_17915"
     CDS             7135..8376
                     /gene="nuoH"
                     /locus_tag="VF182_17915"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX6439015.1"
                     /translation="MWTRRRLTGKAKLKIMQAYLDQLMRDGAFAGMPKEVVYTIGMLL
                     AAFIVLSVFVAPLAGVTSWLERRVWARMQSRVGPNRVGPQGILQWLADGIKNLLKEDI
                     VPSAADSKLFSLAPYIVFMGFLCSFVVIPFGGKLIVADLNIGILYLLAVTSLVVVGIL
                     MAGWASNNKWSLLGGMRSAAQIVSYEIPAGLSVLMIVFLAGTLSMQGIIQGQGWAPWD
                     WYLFHNPFTFAGFFLYFTAALAEGNRTPFDIPEAESELVAGYVTEYSGMRFLFFFFAE
                     WGNLYVIGAVATTLFLGGWQVPPLGIFQNSPVLLALAQFITFFLKAYLWVFVAMWIRA
                     TLPRVRVDQLMTMCWKYMVPLSFICLLGTVAFMFVPADLQRIISAAMFGLGVAITIIF
                     FLRVSFQIRQAKPELYFKPHI"
     gene            8393..8950
                     /locus_tag="VF182_17920"
     CDS             8393..8950
                     /locus_tag="VF182_17920"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEX6439016.1"
                     /translation="MGVVSYVRDIKDTVKTVFEGLSITFSHLVRRPMTIQYPDKIPIP
                     IQETLPRRYRGILEVDLDICTGCLACERVCPITCITIGIDMDKETKQRQFTHFDIDIC
                     KCMYCGLCSEVCPTGAIRHSQEFEGASYNPAGLVRHFAPEPSALYKPKKGGETDPTLV
                     VKVDIGLKYLDEFATPDGGTVTDEK"
     gene            9066..9566
                     /locus_tag="VF182_17925"
     CDS             9066..9566
                     /locus_tag="VF182_17925"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX6439017.1"
                     /translation="MDISTAVFYLIAIITVGSAAVVAFSPNIIYSAFSLLGTFAGVAG
                     LYVFLGADFVAGVQVLIYVGGILVLILFAVMLTHRITDVEITNRAAGRIPGLLIVAVF
                     LVLLIQTIRETPWVRVKEIAHQPTTAKIGDLFLENYLLPFELASLVLLAAMIGAVVLS
                     RKEIKE"
     gene            9569..9886
                     /gene="nuoK"
                     /locus_tag="VF182_17930"
     CDS             9569..9886
                     /gene="nuoK"
                     /locus_tag="VF182_17930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX6439018.1"
                     /translation="MVFSPNELTSYLIIGAVLFSLGLLCVLTRRNAISVLMGVELILN
                     SANINYVAFSHFGSGNLDGQIYAMFVIMLAAAEAAVGLAIVLAIFQLFHTIDVEAAQT
                     LKE"
     gene            9915..11948
                     /gene="nuoL"
                     /locus_tag="VF182_17935"
     CDS             9915..11948
                     /gene="nuoL"
                     /locus_tag="VF182_17935"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX6439019.1"
                     /translation="MDQPIQNDFLRWIIFLPLIGAIINGLLGLKIQRRMGKGAIAVIA
                     CAPVIIAFALSVLAFFQLKALPPEQRFLIDNLYSWIDLGSLKVNMAFLVDPLSAVMIL
                     IVTGIGGLIHIYATGYMHEDEGFWRFFAYLNLFTAAMLTLVLGDSLLLMFVGWEGVGL
                     CSYALIGFWYQDHANARAGNKAFIVNRVGDFGFVLGMFLLFWSLDAQGHGTLTFREMA
                     RWASTLEGQTIWGFSVATVATLLLFVGATGKSAQIPLYVWLPDAMAGPTPVSALIHAA
                     TMVTAGVYMTARLNVFFSMAPFTLQVIAVIGVMTALLAATIALTQTDIKKVLAYSTVS
                     QLGYMFIGIGVGGYAAAVFHLMTHAFFKACLFLGAGSVIHAMHHEQDMRKMGGLKNYM
                     PLTFFTFLISVLAISGTPGTAGFFSKDEILWLAFSNHHPVIWALAIVGAGMTAFYMFR
                     QLFMVFFGECRADHHTQEHLHESPRVMTLPLIILAGGALLAGWIGLPAVFGGSRFAEW
                     LEPVIAHAEHHGSHELELGLMGISIAVAATGALLGYLIYYRGALSPSRFANFAGGFFY
                     RLFDNKWYLDEIYQAVFVNGTLLLARVFSAFDKYIVDGIVNGTASLTRFVSWLNGLFD
                     HYIVDALVNALANVTFWAGNKFRRVQTGNINSYLYGILIAVVLAIIIKLRYWS"
     gene            11974..13497
                     /locus_tag="VF182_17940"
     CDS             11974..13497
                     /locus_tag="VF182_17940"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX6439020.1"
                     /translation="MEQNVLTYMIFIPLAGAVVVLLLPGKGYNLIRWVSAAATVPPLL
                     MGCWLYANFDTSQTGFQFVQKLPWIPAYNIQYFVGVDGLSISMILLTALLSFLCIFAS
                     FGIDRGVKGYFALFLMLEAGMMGVFAALDFFLFYIFWEVMLLPMYFLIGIWGGPRREY
                     AAIKFFLYTLFGSVLMLLAMLALYFNTSPNTFDMTVMMAQANTYSTQILPFWPFIDLR
                     WGFQHVVWMALFIGFAIKIPAFPFHTWLPDAHVEAPTAISVILAGVLLKMGTYGILRI
                     NYPMMPAATADMAYWLLGALGAWNIIYGALCAMAQSDLKKLVAYSSVSHMGYVMLGMA
                     SFTTQGINGAVLQMFNHGTITAMLFLLVGVIYDRAHHRDIEGFGGLAAVMPLYTGVTA
                     LAVFASMGLPGLSGFISEVLVLLGTWQKYPVLAILGATGVILTAGYLLWMIQRVYLGP
                     VNEKYKALPDVSSRELFTLIPLGAIVILVGVYPSVVLDLLRASLDNINQIVIPHLPS"
     gene            13504..14988
                     /locus_tag="VF182_17945"
     CDS             13504..14988
                     /locus_tag="VF182_17945"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX6439021.1"
                     /translation="MDLNNLESLNFFGPELILTGTIILLIALDLFMRNKRNLAVVAII
                     GCVASLLSAFDLYSAQQGWLFYRMIVLDNFSLFFKIVTLLAAIFAIWMSLGSAEIRQV
                     YQGEYYTLLLTCALGIFFMASSTNLLTAYLSLELVSLTSYVLTGFLPRNRRSSEAALK
                     YLIYGGVASGTMIYGMSWIFGMAGSLDYAAIQQVLAKSELNKTGLFMAFMFVFAGFGY
                     KIVFVPFHMWSPDVYQGAPTPFTAFLSVASNAAGLAILIRFFYPGISSVTAAGDWAFL
                     PGVEWPQILLFASMITMTVGNLCALNQRNLKRMLAYSGIAHAGYMLMGLAILTNDGLS
                     AILFYIVVYLIMNVGAFLVVAMVANHIGSEDIESYRGLAWRGAVVPAVCFSIFLFSLT
                     GLPPFAGFIGKFFLFAAVLKEGGEFIILALVAVANSVVSLYYYAKVVKVMFLDTPGPG
                     DQAVGIARHNFTLMVPLAVLTVVLGIYFSPLSQYTTQSLRFFVK"
     gene            complement(15039..15659)
                     /locus_tag="VF182_17950"
     CDS             complement(15039..15659)
                     /locus_tag="VF182_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6439022.1"
                     /translation="MLKIWGRTDSSNVQKVLWCCSEIGLEFNRVDWGGKYGGNKDQAY
                     RDMNPNGLVPTIQDGDFVLWESNSIMRYLVEKYGRGKLLPATPEDRANANRWMDWQLS
                     TFNPIIVPLFFATIRTPPDKRDPKAIEAALDKSSQAWQIVDNHLEGKKYLAGQEFSIG
                     DIPLGVWVHRWFNLPVERPEQKNLSAWYERLTQREPYQTHIMIPVT"
BASE COUNT         3247 a         4057 c         4311 g         4315 t
ORIGIN      
        1 aaccttcttc gcgtgcaagc tgagcgcgct tctgcgtcac tccggctacc acgtcggcgt
       61 gatgaagccg gcggagagcg gctgcgctga acgacaaggt aaactttttc ccgaagacgc
      121 gattcgttta aaagaggcgt ctggctctca agattcgctg gataaaatat gtgcttatca
      181 attgcgtgaa ccgctggcgc ccagtgtggc ggcacaacgt caaggcatca cgctgaagat
      241 tgataaattg atggatgtct atggcgaaat cagttcaggc cacgacatca cgcttgtgga
      301 aggcgccgga ggactaatgg taccgctatt gcccagctac acctacgccg attttgccag
      361 agttctcaaa cttccgatga tcgtcgttgc cgccaatcgt ctcggtatga tcaatcatct
      421 gctgctgaca cttgagcatg ctcgctgcaa aggtctgagt gtgctgggtt atgtcctgaa
      481 ccgactggaa aacggcgcga cgctggcggc ggagaccaac cgtgaagcgc tttcaacgct
      541 tacggcagtg atttgcgccg gagaattgcc ccatgtggcg ccagcggaag atgctcacaa
      601 tggtggcgcg gactggttcg ccgaacagtt tgatctccgg attctaaggt ccgtgttatg
      661 tgagcgatca cgtcagtgac cagcttagct gatagcctat ctcttcaagg ggctagagaa
      721 gctcgatgcc tgaaatgcgt tctggttggc tctgatgaat tctcgataga gaaccgccgc
      781 tcaatgtaaa taagcgctaa tcgccgccca gcgaggattt tccatagttg gagtttttcg
      841 agggcgtttg gtgtcggcgc gcagctacgg cgtgttactg cttgaagctt ttgacgattt
      901 cgtccctcaa gaccttgagc ggaatgtttt tctcgatggc gatgcgcttt aactcctcgt
      961 attcaggact aatgcgcttt tcgccgttgg gttcttgcca gatctttacc ttcacatcgc
     1021 caaagcgtgt tttaacggtt gcctcggttc tgttgaggat gatgcggttc acggaatgat
     1081 agcgcaggcc aatggtggtt gtttcctcga atagtatttt tgccagcctg tcccgattgc
     1141 gcggctcggc gatgactcgc acaagcacgg cgggacgatt tttcttcatt tgaatcggcg
     1201 agaaaaatac gtctcgagcg ccggtggcga atagccgatg catgacatgc tcgaaaaact
     1261 gcggattcat gtcgtcgatg ttggtttcga tgacgagcat ttcttcctgc cgccaatcgg
     1321 tgctggcacc gagaaccaac cgaaataggt tcggccgatc gggccactcc ttttgtccgg
     1381 tgccgtaacc gatcgtttcc accgtcatgc tgggttcttc gccgaagctg gtccctaata
     1441 cggacatgat tgcggctccg gtgggagtga cggtttcgcc ttcgagctcc gtccattgaa
     1501 cggggatgcc tttcaggagc tccagggtcg caggtcccgg caccggcagc gggccatggc
     1561 cgctctgcgc ccgaccgcgt cccagcggga ctttggaaaa atgcaaagca ccgatcttga
     1621 gctcgtgaaa gccgattgct gcggcgagaa tgtcgacaat cgaatcggtg gcgccgattt
     1681 cgtgaaagtg aactgctgcc gtgggcacac cgtggatttt gccctcgact tgggccaggc
     1741 gctggaatat cttgagcccg gtttcttgga cctcgggcgc gagcttgctt gcttcgataa
     1801 gctgatgaat ctcctcccag gaccgcggcg cttcattgct cggcggcctt acctgaaact
     1861 gtgtggcagc gatagcgtgg gtagtcttct ttgcagcatc gagtgcgaat tcaagcgtgg
     1921 caatttttcg caattcccga gtaagaagtt caagcggaag ccccaggtcc agcagggcgg
     1981 cgacgaacat gtcgccacta attccaccga ttagatcacc ataggcgatg cgcatttaat
     2041 ttacctggaa gagccgtttc gcgtgttggg tggggaaaat tgccgcatcg ttcgcatagc
     2101 cgaaagaatg actcggctac caatctgaat ggatctcttt gccactggtc tcgacaaaga
     2161 ttttctcaga cttaaacggc ttgtcttgca agcgatcgac gagccaattg atcaacaggg
     2221 atcgcgaacg tgggttgcgg atcgagtgcc gctctccttc aaatatgtat agaagtttcg
     2281 gagcgggaat ttcaccgatg agctcataga caaacttcat atcgcaatgc tcatcgtcct
     2341 cgccggcaac gaccatgtag gggcagctta ttttcgctgt cacgcctttc aaagtcagcg
     2401 tcttggcaaa ctcatcgaag gcagcttcat cctcgtaacc ggacatgtac atgtagttaa
     2461 gtttgaacgt gggggatgac atattgaaaa tggcgtactg gcccggctcc atgcagacgc
     2521 cgcttactgc gcaggctttg aaacgttttt cttcggccac aactcgcggc gcccagtacg
     2581 accccatgct cgaagcaaga atccccagcc ggttggggtc aatctcggat cttttgacca
     2641 ggtaatcaca ggcaagctta cccgcctcgg cataattcga tgcgctacag gtgagacccc
     2701 cttcacgcgt ttcgccctga cccggaccgt caatggcgag cacggcgata ccgcgttcaa
     2761 gaaacggatc gccggttgcg gggttgtcct ctttgacgcc atccattccc gggatgtaca
     2821 tgacgcacgg cactttctcg cctgctttcc tctcgggggg cagatgcaga agtgcatgga
     2881 ttagtttgcc ttgatatgga agttctattc gctcgatggg acggccggcg tagcgaatga
     2941 atttgtcata acaggcgcgt tttttttcct gccaattcat ccgcttggga tttctctctt
     3001 catacactgc ccacatcgca ttggtgtaaa agcacgccgc aatatagaaa tgatcgcgag
     3061 cttccggcag atgtcctgac ccttcggctt cgtatgcctg ctgttcacgc cggcttgcgg
     3121 cgcgggcgaa ctcgcgcggt atgtcaacga acttctgaat tcttcggcaa acttcttgaa
     3181 ggtcgctttg aacgcccggg ccgcagttgc gcagaatttt acccgtccgt ccctggtccc
     3241 agtcgatccc acgagcctgg atgacctgat cgagcagcca gcgctgctcg cgccagcgtt
     3301 tcattttacg ttctgtttcg attctcatac ggcctccgaa caacgtcgag acaagtctca
     3361 ggatgaagat gccgaaaccc gattcgtcct ttctgctgcg caataggtca gcgcggcttt
     3421 acaaagcgat catcgatgaa cattttcaag tcgatttgct tgagctgcgg cgctgaatcg
     3481 accaggctgt cgcgcacagc ttccagcgct gtaagtcccg gatatggcgg gctggaaatg
     3541 gcggcgtcgt attctttgta gccctgttcg agtattcggt cctcggcgcg catgtatttt
     3601 cgtaaaaccc ggaccgcggc accgctgtct tctttgtaga tcctcgcgcc gtcggtaagc
     3661 gctgccataa agtctctgac gatattgggc tcggcttgta cagaagtcct tcttgccgca
     3721 atgcctgaaa aagtaaattc ctttcccagg tcggggatgt gaacaagcgg acgatagccc
     3781 aagtcccggg ctgccgtcga agtcggcgga gagagaattc ctccacttac ggctccggct
     3841 ctcatgccgg ccagaatatc cgggatccct cccattgaga tcacggcaac atctttgatg
     3901 ctcaacccga gatgttctag agccgttcgt aaggcgagat cgcttgccga accgatacga
     3961 gtaatcgcga gcttctttcc tttgaggtga gctgcctgag tgatagttgc atccgtgatg
     4021 agggtgaaga ccatctgatg gatggtcgtt cccacccacg cgatatcagc gccctggacc
     4081 caagccgtca tcataggtgc cggtgaaaga tggccaaact ggatctcacc cgccagcaac
     4141 gcttgcgccg cagtagtacc gctgcgcatg tatactaaat tggtttctag gccatatttg
     4201 cggaaaatcc cttgttcatg ggtcaaccat agaaccccaa tcccggccga caaggcgctg
     4261 taggcgacgc gtaccggcct cggtttagtt tgaccgcggg attcccccga gccgaccgcc
     4321 agaaaagcga cgaggacgac cacgatgatc ctgcagtttt cggtcgggtt catgaaactc
     4381 ctgccgcggt ctacctggtc tgctggaatg gttgttgtac gtagttggtt gtgagcgctc
     4441 gagaccgcca cgactctagt caaaaaagct agcaatggca aatacatgag accgaaccgg
     4501 tgctatcgaa ggttgattct ccgtggtatt tatggttata aggaaacgtt acctttcttt
     4561 aactattaac tagaaactta cgatgctttt tgattttgct aatgtcctag tgtttacggc
     4621 attagggctt ggattcgtcg gagtgaacct attgatcggg cgtttgcttc ggccggcaaa
     4681 tcctcaagcc cgcaaactct cgacctatga gtgtggtgag ccgtccacag gcagcgcctg
     4741 ggtcaacttc aatattcgtt tttatctcgt ggcgttgatt tttataattt tcgaggtgga
     4801 aatcgccttt atttttcccg ttgccgtcgt atttcgtaac ctcgtcgaga gcggcgaggg
     4861 actgttcgca tttatcaaaa ttttcatatt tctcggcatt ttatttttgg gtctgattta
     4921 ttcgtgggtc aaaggcgatc tcgactggat caagaaggtt agggtatagc cggcgatctt
     4981 cggagggtaa atgaaaccac tcattaattc acttcctgaa acggtgttta ccactaaagt
     5041 ggatgacttg cttaactggg ggcgggcatc ctcgcagtgg tacatgttat tcggcttggc
     5101 gtgttgcgcc atcgaattga tgcaaaccgg cggacccagg gccgatttag atcgattcgg
     5161 cgcggttcca cgggcgaccc cgaggcaatc ggatctgatg atcgttgccg gaacgctaac
     5221 gtataaaatg gcgaaacgga ctaagcttct ttacgatcag atgccggatc cgaaatatgt
     5281 catttcgatg ggtagctgct caaactgtgg cggtctcttc cagctggcct actcggtgtg
     5341 caaaggcgtc gataaaattg ttcctgtcga tgtctatgtg ccgggttgcc cgccgcggcc
     5401 agaggcgcta acagaaggtt tgttgcggat tcaagataag atgatgcgtg agcgttggct
     5461 ggtgaagtca aatagtgagc cggagtccgc ggttcaggcc taagtatgca acccgctgaa
     5521 attttttcgc gactcgaaaa acaattttct ggaaaagtga gtggcttcaa ggccgatgtg
     5581 ccggagcctt atctgtttgt taacgccgaa gcgattgtcg aggtctgccg gtttctacac
     5641 gatgatgccg acttgaagtt cgaggtgctt tcggatcaaa cggcagtgga ttggccaaag
     5701 gaagaaaaaa tccagctcgt ttatcatctt tattcataca gcgaccggca tcaggttgtg
     5761 ctcaaggtgg acctgcctcg agatactccg aaaatcgcga ccgttgagaa catttggaaa
     5821 gttgctaatt ggttcgagcg cgagatcttc gatcttttcg gggtcatctt cgaaggccat
     5881 agcgatttgc gccggatcct cttgcctgaa gattgggagg gatttccctt gcgcaaggac
     5941 tacatcgagc aggaagaata cgacggcatc agcacgcaac gcgccccgct ggtagaaaag
     6001 ctgctgcgat agggatttcg atgaacgaga ttgttcagga tcaacacgcc ggattttata
     6061 ccgaggacat gaccatcagc gtcggcccgc agcacccgag cacgcacggg gtcctgcgct
     6121 ttgtagtgaa aactgacggc gaggtcatcg gtgaagcgat tccggatgtg ggttatttgc
     6181 atcgttctat tgaaaaaata ggcgagaagg taacttggca cggctacgtg ccttataccg
     6241 atcgcgcgga ttacttggca gccatgtttg ccaatcaagt tttctgcatg gcagccgaga
     6301 aactctcagg aacgccggtg actcgccgtg gtgaattctg ccgggtaatc gcctgcgagc
     6361 tcaatcgcct tgccagccac ctgatttcgg tcggaacttt cggccaggac atcggtgcga
     6421 tgacgccgtt cgttcatgcg ctgcgcgagc gcgaaaccat caataatttg atggaggaga
     6481 tctgcggcgc gcggctcacc tacaattaca tccgcatcgg cggtgtcggt tatgatattt
     6541 taccggaaac ctgcgcgcga attgctgagt tcctcgatca tttcgagccg attgtccatg
     6601 agttcaaccg attgctttcg gaaaacaaga tcttcatcga acgtttagct ggtgtttccg
     6661 tggtgtctaa agaggacgcg ctgcaataca atctggttgg tccgaaccta cgcggctccg
     6721 gggtcaaatg ggacatccgg cgcgacatcc cttattccgt ttaccccgaa ctcgacttcg
     6781 atattccagt aggcacaggt cacatcggca ctctcggaga tagcttcgac cgttattggg
     6841 tgcgcatccg tgagctgcaa gagagttgca agatcattcg gcaatgtttg aggatgctgc
     6901 ctggcggacc ggcaatttcg aaagtaccgc ggaaattcaa accacctgcg ggcgaatgct
     6961 atgttcgtgt cgaggcgcca cgcggtgata tgggctttta tgtcatcagc gatggcagcg
     7021 aataccctta tcgagtgcgg attcggaccg gttcctatac cgcgatgagt attatcgata
     7081 aactgagtaa ggggattatg ttggcggatc tcatcgtcct gatcggcagc ctcgatgtgg
     7141 acgcgccgga gattgaccgg taaagccaaa ctcaaaatca tgcaagctta ccttgaccag
     7201 ttaatgcggg atggcgcttt tgccgggatg cccaaagagg tggtttacac tatcggcatg
     7261 ctgctggcag cctttatagt tttgtcggtt ttcgttgcac cgctagcggg cgtcaccagc
     7321 tggctggaac ggcgagtctg ggccaggatg cagtcccgtg tcggacccaa ccgtgtaggg
     7381 ccgcagggaa tattgcagtg gttggccgac ggcatcaaga atcttttaaa agaggatatc
     7441 gttccctctg ccgctgattc gaagttgttt tcgcttgcgc cttatattgt ctttatgggt
     7501 tttctctgca gtttcgtggt catccccttc ggggggaaac tcatcgttgc cgatttaaac
     7561 atcggtattc tttatctttt agctgtgacc tcgttggtgg tcgtcggcat tctgatggcg
     7621 ggctgggcct ccaataataa atggtctctg ctgggcggaa tgcgttccgc tgcgcagatc
     7681 gtcagctatg aaattccggc gggattgtcg gtgctgatga ttgtttttct cgccggcact
     7741 ctgagcatgc agggtatcat acagggacaa ggctgggcgc cgtgggactg gtacctgttc
     7801 cataatccgt ttaccttcgc tggatttttc ctttacttca ctgcggcctt ggctgaaggc
     7861 aaccgcaccc ctttcgatat accggaagcc gaatcggaac ttgtggcggg atacgtcacc
     7921 gagtatagcg ggatgcgctt tctttttttc ttttttgcag agtggggcaa tctatatgta
     7981 atcggcgcgg tggccaccac gttgttttta ggcggatggc aggttcctcc gctgggaatt
     8041 tttcagaata gcccggttct cttggctctg gcccagttca ttacgttttt tctgaaagcg
     8101 tatctttggg tatttgttgc gatgtggatt cgcgcaaccc tgccgcgagt tcgggtagat
     8161 caattgatga cgatgtgctg gaagtacatg gttccgctct ccttcatctg tttactggga
     8221 acggtcgcgt tcatgtttgt tccggccgat ctccagcgga tcatcagcgc cgcgatgttt
     8281 ggattaggcg ttgcaattac gatcattttc ttcctccggg taagcttcca aattcgccag
     8341 gccaagccgg agctgtattt caagccgcac atttaatgat tgttggggga ttatgggagt
     8401 tgtcagctac gtaagggata ttaaagatac ggtaaagacg gttttcgagg ggctgtcgat
     8461 taccttctcg cacttggtgc ggcgtccgat gacgattcag tatccggata aaattccgat
     8521 accgattcaa gagaccttgc cgcgccgcta ccgtggaatt ctcgaagtcg atctcgatat
     8581 ctgcaccggc tgtttggctt gcgagcgcgt ctgcccgatc acctgcatca ccattggcat
     8641 cgatatggac aaggagacca agcagcgcca gtttacccat ttcgacatcg acatctgcaa
     8701 atgcatgtac tgcggactgt gctcggaggt ttgtcccacc ggtgcgatcc gtcattctca
     8761 agagttcgaa ggagccagct ataatcccgc cggactggtg cgccactttg cgccggaacc
     8821 ctcagcgctc tacaagccca agaagggcgg ggaaacggat ccgactctcg tcgtcaaagt
     8881 ggacatcgga ctaaagtatt tagatgagtt tgccaccccg gacggcggaa ctgtgacaga
     8941 tgagaagtag ctgttgatgc ggaggcggag gcgggtcgat gcgcgatagg ccttgatacc
     9001 aaatcaatct tcctgtattt cagccaaaag ccttctgcct ttcgattcct aaaatttaac
     9061 cagtgatgga tatctcaacg gcggttttct atctgatcgc gatcattacg gtgggttccg
     9121 ccgcggtagt ggcattttcg ccaaacatta tttattcggc gttttcactt cttggaactt
     9181 ttgccggagt tgccggacta tatgttttcc ttggcgccga tttcgtcgca ggtgtccagg
     9241 tgctcatcta tgtcggcggt attctagtcc tcattctatt cgcggtgatg ctgacgcatc
     9301 gcattaccga cgtcgagatc accaatcgcg ccgcgggccg gattcccgga ttgctgatcg
     9361 tcgcggtttt cttggttctt ttaatccaaa ctattcgaga aactccgtgg gtgagagtga
     9421 aggaaattgc ccatcaaccg accaccgcca aaatcggcga tctattcctg gagaactatt
     9481 tgctgccttt tgaattggcg tctttggtgc tgctcgcggc aatgatcggt gcggtggttc
     9541 tttcccgcaa ggagatcaaa gaatagccat ggtgttttcg cccaacgagc tgacctccta
     9601 cctcattatc ggcgcggttc tcttctcgct tggcctacta tgtgttctta cccggcgcaa
     9661 cgcgatcagt gttttgatgg gcgtggagtt gattcttaac agtgcgaaca tcaactatgt
     9721 cgctttctcc catttcggca gcggaaatct tgacggccaa atctatgcca tgttcgtgat
     9781 catgctggcc gcggccgaag ccgcggttgg cttagccatc gttttggcga tctttcaact
     9841 tttccatacg atcgatgtcg aagccgcaca gactctcaaa gaatagcgcg catgttgtag
     9901 gatagaaaag ctccatggac caacccatac aaaatgattt cctgcggtgg attatattcc
     9961 tgccgttgat tggagcgatc atcaatggtc tcttaggcct taagatccaa aggcgcatgg
    10021 gcaagggcgc gatcgcggtc atcgcttgtg cgccggtcat catagccttc gcgctatcgg
    10081 tgctcgcttt ttttcaactc aaggcgcttc ctcccgagca gcggttttta atcgataatc
    10141 tttacagctg gatcgatctt ggctcgctca aagtgaatat ggcttttttg gtggatccgc
    10201 tttctgcggt gatgattctg attgtgaccg gcatcggcgg attgattcac atctacgcta
    10261 ccggttacat gcacgaagat gaagggtttt ggcgtttctt cgcctatctg aatttattta
    10321 ccgccgccat gctgacgttg gttttgggcg acagtttgtt actcatgttt gtcggttggg
    10381 agggggtcgg cttgtgctct tacgcattga tcggtttttg gtaccaagat cacgccaatg
    10441 cgcgagctgg aaacaaggca ttcatcgtca accgagtcgg tgattttggc tttgtcctcg
    10501 ggatgttttt gcttttctgg tctctcgatg cgcagggaca cgggaccttg actttccgcg
    10561 aaatggcgag atgggcatcg accttagagg gtcagacaat ctggggattc agtgtggcga
    10621 ccgtcgctac cctgctttta tttgtcggcg ctaccggtaa gtccgcacag atccctctat
    10681 acgtttggct tcctgatgct atggccgggc ctactccagt cagcgctttg atccatgcag
    10741 ctacgatggt gactgccggc gtttacatga ccgctcggct gaatgtcttt ttttccatgg
    10801 cgccctttac tttgcaggtc attgccgtga tcggagtgat gacggctctt ttagcggcga
    10861 cgatcgcact gacccagact gatatcaaga aagtgctggc ttactccacg gtgagccaac
    10921 tgggctacat gttcatcggc atcggtgtag gcggctatgc agcggcggta tttcatttga
    10981 tgacccatgc gttcttcaag gcttgtttgt tcctgggcgc cggcagcgtt attcacgcca
    11041 tgcatcacga acaagacatg cgcaaaatgg gcgggctaaa aaattacatg ccgctgacat
    11101 tttttacttt cttgatttca gtactggcta tctccggcac gcccggaacc gctggatttt
    11161 tctccaagga tgaaattctt tggctggcgt tctccaacca tcacccggtg atatgggcct
    11221 tagcgatcgt cggcgccggc atgacggcct tttatatgtt tcggcagctg tttatggttt
    11281 tcttcggtga gtgccgcgcc gatcaccata cccaagaaca tctgcatgaa tcgccgaggg
    11341 ttatgacgct gccgctaatt atcttagccg gtggcgcctt gttggccggg tggatcggcc
    11401 tgccagcggt ttttggtggc agccgatttg ccgagtggct cgaaccggtg attgcgcacg
    11461 ccgagcatca tggctcgcac gagttagaac tgggcttgat ggggatctcg attgcggtgg
    11521 ccgctacagg agcattgcta ggttatctga tttactatcg aggggcgctt tcgcccagtc
    11581 gctttgccaa ttttgccggt ggattttttt accgtttgtt cgataacaaa tggtacctgg
    11641 atgaaattta ccaggctgtt ttcgtcaatg gtacgttact gctggcgcgg gttttttcgg
    11701 ctttcgataa gtatatagtc gatggcatcg tcaatggcac ggcaagcttg acgcgtttcg
    11761 tctcgtggct gaacggcctg ttcgaccact atatcgtcga tgcgctggtc aatgcccttg
    11821 ctaacgtaac cttctgggcc ggcaataaat tccgccgagt tcaaacgggt aacatcaaca
    11881 gttatctgta tggaattttg atcgccgtgg tgctcgccat catcatcaag ttgcgctatt
    11941 ggagctgagt tctgacgggg gagaatgtag aagatggaac agaacgttct cacttacatg
    12001 atttttatcc ctcttgccgg ggcggtggtc gtgctgttgc tgcccggcaa gggatacaat
    12061 ttgattcgct gggtttcggc ggccgctacg gtgccaccat tgctgatggg ctgctggctc
    12121 tacgccaact ttgacaccag ccaaactgga tttcagttcg ttcagaagct gccctggatc
    12181 ccggcttaca acattcaata tttcgtcggt gtcgatggtc tcagtatttc catgattctt
    12241 ttgacggctc tcctgagttt tctgtgtatc ttcgcatctt tcggtatcga tcgcggcgtg
    12301 aaaggatact ttgcgctttt cctgatgctc gaagcgggga tgatgggcgt gtttgctgct
    12361 ctagattttt ttcttttcta tattttttgg gaagtcatgt tgcttcctat gtacttcctg
    12421 atcggcatat ggggcgggcc gcgtcgtgag tatgccgcca tcaagttttt tctttacact
    12481 ctgttcggca gcgtgctcat gttgctggcc atgttggcgc tctacttcaa taccagtcct
    12541 aacaccttcg atatgacggt gatgatggcg caggccaaca cctacagcac gcagatcctc
    12601 cccttttggc cgtttattga cttgcgctgg ggctttcagc acgtggtttg gatggctttg
    12661 tttatcggct ttgccatcaa gattccggct tttccatttc acacctggct tccggatgcg
    12721 cacgtcgagg cacccaccgc aatctcggtc atcttggccg gcgtccttct caaaatgggt
    12781 acctacggaa ttctccgcat caattacccg atgatgccgg cggcgacggc ggatatggct
    12841 tattggctac taggcgcgct cggcgcctgg aatatcattt acggcgcgct gtgcgcgatg
    12901 gcgcagagcg atcttaagaa actggtggct tattcgagcg tcagccatat gggctatgtg
    12961 atgcttggca tggcgagttt tacgactcaa gggatcaacg gtgccgtgct gcagatgttc
    13021 aatcacggca cgatcacggc catgctcttc ctcttggttg gcgttattta tgatcgggcg
    13081 caccatcgcg acatcgaagg ttttggcggt cttgccgcgg tgatgccgct gtataccgga
    13141 gttaccgccc tggcggtatt tgcttcgatg ggcttgcccg ggctttccgg atttatctcg
    13201 gaggtgttgg tattgctcgg cacatggcag aaatatccag tgctggcgat tcttggcgcg
    13261 accggtgtga ttcttacggc gggttacttg ctctggatga tacagcgcgt ttatctcggc
    13321 ccagtcaatg agaaatataa ggcattgccg gatgtgagca gccgggaact atttacttta
    13381 atcccgctcg gagccatcgt cattctggtc ggagtttacc cgagcgtggt cctggattta
    13441 ttgcgcgcct cgctcgataa catcaaccag attgtcattc cgcacctgcc ttcctagcag
    13501 accatggatc tgaacaactt agaaagcctg aactttttcg gcccggagct gatcctgacg
    13561 ggaactatca ttctgctcat cgctttggac ttgttcatgc gcaacaagcg caatctagca
    13621 gtagtggcga ttatcggttg tgtcgcatcg ttgttgtctg cctttgatct ttacagcgcc
    13681 cagcagggct ggcttttcta tcgaatgatt gtgctggaca actttagcct gtttttcaag
    13741 atagtcaccc tgctggcggc gattttcgcg atttggatgt cgctcggcag cgctgagatc
    13801 aggcaggtgt atcaaggtga gtactatacg ctgctgttga cgtgcgctct cggtattttt
    13861 ttcatggcgt cctcgactaa tcttctcacc gcctacctat cgctggaact ggtaagttta
    13921 acctcatacg ttctcacggg ctttttaccg cgcaaccgcc gctcgagcga agccgcctta
    13981 aaatatctga tttatggagg cgtcgcgtca gggacgatga tttacggcat gagctggatc
    14041 ttcggtatgg cgggcagctt ggattatgcg gcgattcaac aggtgcttgc caaaagcgag
    14101 ttaaacaaaa ccggcttgtt catggccttc atgtttgtat ttgccggctt cggatacaaa
    14161 atcgttttcg ttccctttca catgtggtca ccggatgttt accagggggc gccgacaccg
    14221 tttacagcgt ttctttccgt cgcctctaat gccgccggcc tggcgatctt gattagattt
    14281 ttttaccccg gcatttcgag cgttacagcc gccggcgact gggcgttttt accgggcgtt
    14341 gagtggcctc aaatcttatt gttcgccagc atgatcacca tgaccgtggg caatctgtgc
    14401 gctctcaatc aaagaaactt aaaacgcatg cttgcttatt cgggcatcgc tcatgctggt
    14461 tacatgctca tgggattggc gattctgacc aatgacggcc tcagtgccat cctgttttac
    14521 attgttgtct atctaattat gaatgtcggc gcgttcctgg tcgttgctat ggtagccaac
    14581 catattggca gcgaagacat cgagagctat cgcggcctag cttggcgcgg cgcggttgtg
    14641 ccggcagttt gcttttccat tttcctattt tcgctcaccg ggttgcctcc gttcgccgga
    14701 ttcatcggca aatttttcct gttcgccgca gttttaaaag aaggcggcga attcatcatc
    14761 ctggcgttgg tcgcggtagc caacagcgtc gtctccctgt actactatgc caaggtcgtc
    14821 aaagtgatgt ttttggatac gcccggtccc ggcgatcaag cagtgggcat tgctcgccac
    14881 aacttcacct tgatggtgcc gttggcggta ctaaccgttg ttttgggaat ctatttctcg
    14941 cctttgagcc aatacaccac gcagtcactg cgttttttcg tgaagtaaag cggagccttt
    15001 ggcgccgttt acggagcacg gactggtcgg ctggcgcatc atgtcaccgg aatcatgatg
    15061 tgggtctggt agggttcgcg ttgggttaac cgttcatacc aggcgctgag atttttctgt
    15121 tccggtcgtt caaccggcaa gttgaaccag cgatgcaccc aaacgcccag cgggatatcc
    15181 ccgatcgaga attcttggcc ggctaaatat ttcttcccct ctagatggtt gtcaacgatc
    15241 tgccatgcct gggagctctt atctaaggca gcttcgatag ctttagggtc gcgtttgtct
    15301 ggcggagttc gaatggtcgc aaaaaaaagc ggtacgataa tgggattgaa cgtggaaagc
    15361 tgccagtcca tccaccgatt cgcattagct cggtcttccg gggttgccgg caggagtttt
    15421 ccccggccgt atttttcgac tagatagcgc atgatcgagt ttgactccca cagaacaaag
    15481 tcgccgtcct gaatagtcgg caccaagcca ttggggttca tatcccgata agcttggtct
    15541 ttattaccgc catattttcc gccccagtct actcggttga attccaaacc aatctcgctg
    15601 cagcaccaga gcactttttg cacattggat gagtctgtgc ggccccagat tttaagcata
    15661 aatacctccc tgcttgtcgc tatattaaga tcaattcctt atcactagtt cggctatagc
    15721 gacaactata tccgatcgag aactcgccgg gcttcgatgg aagttttccg aacattagcc
    15781 gcggaattgc cgatatacgc tgtgccacgc gttctgactt tgaacgtcta ccgtttcaat
    15841 tacatatctg ctacacaaga gaagtttatt tttgccgaag atagtccagg tcatgctgca
    15901 gcgaattagt cttttaggtt tgacttccat
//
LOCUS       DASUVS010000335        15065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1888845, whole genome shotgun sequence.
ACCESSION   DASUVS010000335 DASUVS010000000
VERSION     DASUVS010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 15065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15065
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1888845"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..612
                     /locus_tag="VF182_17955"
                     /pseudo
     CDS             1..612
                     /locus_tag="VF182_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263013133.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin family protein"
     gene            1263..2405
                     /locus_tag="VF182_17960"
     CDS             1263..2405
                     /locus_tag="VF182_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006233874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX6439023.1"
                     /translation="MVRVLTYSVLLIVGMVLSQLPAIDHLKPEIAALTMIFLAYIMIQ
                     VGMEFEIDKNDLRKYGVDYLVAMAAAAVPWLTAAAYFWCFFHIGTAESLLIGRFAAPT
                     SAGILFTMLAAAGLAATWTYKKARVLAIFDDLDTILLMIPLKMMLLGFTLKLVGLGVV
                     VIVLLLAAYFMIHWLRVPTNNFWVAGYAALVWGSCVAFDYVTELHLEVLLPAFALGCL
                     IKSDHLHGSEEFDPHARGPEPCNREREDVLDRFIKGSFMLLAGMALPPIQFGAIGIGI
                     VVVHVLVLTFLMNIGKCVPLAAYGDQASLRERAALSIGMFPRGEVGIGVLLVSLEIFH
                     QTGSGDIPGISESISLGGLSLALNLCLTGLFILAVIRLLNSATAND"
     gene            complement(2778..3239)
                     /locus_tag="VF182_17965"
     CDS             complement(2778..3239)
                     /locus_tag="VF182_17965"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020482166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX6439024.1"
                     /translation="MRVLVLKVPPLAVGVLLAALMWLVSRAMPALSFVFPGRKSLALS
                     FAITGAMIIIVGVASFRRAKTTINPMKPESSSSLVVSGIYKFSRNPMYLGFLLVLVGW
                     AVFLSNALAFIFLPVFICYMNRFQIEPEEKALAGKFGQEFVDYKSRVRRWL"
     gene            complement(3249..3959)
                     /locus_tag="VF182_17970"
     CDS             complement(3249..3959)
                     /locus_tag="VF182_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6439025.1"
                     /translation="MSDHATALVIDDEKQIRKFLRIVLEEDQYRVLESENGKQGLADV
                     ALRRPDVVLLDLGLPDIEGITVLKRLREWSQVPVLILSVRDGPEDKVAGLDAGADDYV
                     TKPFEALELLARLRAIQRRSNSAADEPFFHAGDLAIDFTNHAITLKGHEIKLTPTEYA
                     LLKILAQHAGKIVSHKQLLREVWGPNAEEQSQYLRVYMTHLRKKIDSLERGEKLLRTE
                     SGIGYRLVIPSKKNQEGA"
     gene            complement(3956..5542)
                     /locus_tag="VF182_17975"
     CDS             complement(3956..5542)
                     /locus_tag="VF182_17975"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEX6439026.1"
                     /translation="MVIGSRDSELVRPRRRRLRLNSLPAWIPSASSVNEFSLAGALIL
                     ALTAAAWLIEPFAGYQAVALLYLLLVVLLGLKLSRGPVLTMAAFSAVLWNFLFVPPYF
                     TFHVQRYHDGIMFLVFFVVALAMGHLTSRLRRSEMLERQRERRTAALYELAQQAAFAT
                     DLDTGLAAAVNLIDSIFAAKAALFVRQKNHSLSAEPHAVSSFDLSDRENGVAAWVFSH
                     RVPAGKFTAYFPDAAALHMPLQGRTAVMGVLAVRPPAAKPWDRAERELLEAFAVLIGL
                     VLEKNHIVEAFKRAEVLEASENLRRALLQSVSHELKTPLSAVQAGIDAITRQTNGDEK
                     KQRTLREVQVAVRRLHRVINNLLDMTRIESGVIQPKLDWCDVEELIQAAIELAGESLH
                     EHHIIVDVDTRLPMVKIDQPLLEQCLCNLMLNAGANSPPGTNITIGAHLAQEQLLLSV
                     LDQGKGIREDDLLRIFDAFYRGVDAAPGGTGLGLAIVGGFVRAHGGTVRAGNGSSGGA
                     EFVISIPVEILRPEIMESLA"
     gene            complement(5633..7633)
                     /locus_tag="VF182_17980"
     CDS             complement(5633..7633)
                     /locus_tag="VF182_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6439027.1"
                     /translation="MSIKSLLLGKPKDPLDPQVFHQVSLVAFLAWVGLGADGLSSSAY
                     GPEEAYLALGDHFFLALPLALLMALTVFVISASYSQIIALFPTGGGGYLVATKLLGPK
                     LGLVSGSALIVDYMLTITISVASAGDQIFSFLPISPEGSKLAVEFGLIFFLIYLNLRG
                     TKESVLFLLPIFLLFVVAHIFAIGLGILPQAAEIPLLASTTYQNTVGDIQGLGLWATL
                     FIVLHAYSLGGGTYTGIEAVSNGLQVLREPRVATGKKTMLYMAASLAFTASGLLLCYL
                     LNQVSHETGKTLNASLFAKIYGGFFSPDLAYVLVIVTLVTEAAILIVAAQAGYIDGPR
                     VLSNMAVDSWVPHRFAHLSDHLVTRYGIWFMGLASLAFLAYSEGDVRVLVVMYAINVF
                     LTFSISQLGMCRHWWRVRHTDKLWLRRLFVNGLGLSFTGTILIITVILKFAEGGWVTV
                     LVTAGFIVVCYWVRRHYDHVRRALKRLDAALMNIPFEPEMKAVPAKSPDGPTAVLIVR
                     DFDGLAVHSLLSIGRLFPNHFKNIVFVSIAVIDSGRFKGLNEIESLSRMKVEDLKSFV
                     DFANCLGWYAEYRYGLGIDLMDELEKLCKSVASEFPGSVFFAGKLVFQEENFLTRLLH
                     NHTPFTLEQRLQFEGHEMMVLPVRVFASLAGGEVARELPACG"
     gene            complement(8369..10207)
                     /locus_tag="VF182_17985"
     CDS             complement(8369..10207)
                     /locus_tag="VF182_17985"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6439028.1"
                     /translation="MQPSKNTRQLNWFLAWAVVFCDIGTSVYYVPGILYGHVKDATPF
                     FVLLTTGGFILLALKYIEISWRNPEGGGVVTITTKAFGPMWGCLGGMLITVDYFLTTA
                     ISAVSGFQYVGSIFSGIDSHIVALACLGVGVLAALNIVGIRESATVALVMACASFLVN
                     LVVIGAAFIGMDASQWQAVLSRLGTGKELSRYELLVGFSSAWLAFSGLESISQLSPAM
                     RLPLRQTTRWAMGAVIVTMVMTAPVLTALSIGLLPEQFKASESERFISELGLIAGGYG
                     IRLAVVLTASSLLLFAANTAIIGSYHVFLALVKGGFLPQIIAVRNPTFNTPHIAVGIA
                     TIVPALVIFFSGGELDILGDMYAFGLLGAFVFSSLSLDSIRWKLGRRDWGFWLGVLTT
                     GMVLVAWAVNLVEKERATYFGGAVTTIGMLIAVGVRRAWFVDLLLQIPFVQRLQARAY
                     RASEDLVEDELKGLMTLTEAVEVKPLYSSSTLLALRDENPRLIKEAIVRARGGGEPAL
                     NCIYVEEWPGLFAGDTPHMPNEQGTQTLKAALQAGRENQIEIVPIWAVSHNAAEAIAN
                     AAKALDVNAVVIGASRRSAFYHMLRGHVVKGLMKKLPHDCRLMIVN"
     gene            10520..11524
                     /locus_tag="VF182_17990"
     CDS             10520..11524
                     /locus_tag="VF182_17990"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439029.1"
                     /translation="MRKSGLFLLLITSTFIGSSDYAPAQVRSSAEKVRVVYSAIGSSQ
                     SPLWIAHEAGLFKKHGLDAEILYVAGGSRAAQVILSGEAPIAMFNGSSVISANLAGAD
                     LVNLASGMNVLPFFLVTGPGIKQIEDLKGGKVAITRFASATDFALRFAAEKWPLKPER
                     DFAVIQLGGQPEMMAALKSGAVQGAMLNAEFTILARREGYRDLVDVASLGLVFPGSGV
                     NTSRSFIKTRRDTVLRVMRAYTESVHLGRTNKSFGVKVLGKYLKNQDVPFLEAVRELY
                     LGHYIPWPPYPSVEAMKRALDDVAERDPRARGMRPEQFIDATLMQELDKEGFIKQLAR
                     "
     gene            complement(11614..12201)
                     /locus_tag="VF182_17995"
     CDS             complement(11614..12201)
                     /locus_tag="VF182_17995"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(11965..11967),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HEX6439030.1"
                     /translation="MKALILLFALMILPIQATVSAQTAAKDGSNPLATDLERVKVGQP
                     APDFTLEDSEGKNVSLADYRGKKNVVLIFYRGHWUPYCVAQLGELQTLLSKQDSAQSQ
                     ILAVSIDSHEDSKKLRQRLQAVNNGSQTFPLLQDTDHKVIDRYGLLNPDGKGWPHPAT
                     YVIDRQGVVRWKFVETNYTIRASNKQILQELKKLK"
     gene            complement(12247..13053)
                     /locus_tag="VF182_18000"
     CDS             complement(12247..13053)
                     /locus_tag="VF182_18000"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6439031.1"
                     /translation="MNFTDRVILITGASDGIGKRLALDLAARGAVIIASGRSPARLEE
                     VLTELRRASPRSIAIRCDVGSRTEVQQMISQVFGQFSAIDILINNAGIGMRKPFLETP
                     LDIIEELMRTNYLGTVYCTYEVLPSMIARGRGHIINISSVAGHIGTLNMAGYCASKFA
                     INGFSESLYHELRPRGIAVSVVSPGPVKTAFNRSFAQTSPKSPETMIITPESVSRRVM
                     TIIENKRFEMITPRSFALMCAIKRFMPGLFRAISYRLFRSHVLPREKAVT"
     gene            13126..14418
                     /locus_tag="VF182_18005"
     CDS             13126..14418
                     /locus_tag="VF182_18005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX6439032.1"
                     /translation="MSSDNGHTYNGGPARRRVVVTGIGAISPNGIGREAYWKSLRCGR
                     SGVDLISQFPTDELSCQIAGEVKNFRAEDYLPPQDLRRVGRAVPLLLAATAEALTDAG
                     IVWQQLSLDERRSWGVIVGSGGGTPDFTEEQYRLFFSNQMRKVSAYNVSGSTPGNVSS
                     EVSLRFDLRGPSHLISTGCTSSTDALGYAFNMIRFGLAEHLVTGGTDATITPGIMQGF
                     CVMRVVSCSRNNEPRCASRPFDRSRDGFVLGEGAWTLVFEERRAALARGAKIYAEVLG
                     YGSTCDAYHRVRLDPNGEEPARAMSLAIQDAGIAKDEIGYLALHGTSTILNDRTETVA
                     AKLCFGRRAYEIPMSSVKSMIGHPQGASGAAGVVAAIMAMNHKFLPPTINCDEPDPEC
                     DLNYIPNRGVARAVDISLCNCIGFGSKNSALVIARGEM"
     gene            14415..>15065
                     /locus_tag="VF182_18010"
     CDS             14415..>15065
                     /locus_tag="VF182_18010"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6439033.1"
                     /translation="MKRSMAREMMDSPDNSPRELLEDDLRNLRIINRYLGNHRVVLGG
                     LARLIEEQKLKRFSLLDVGTGSADIPALIASWARRYHLIARLIGLEPEPVTLRAAASQ
                     IRDYSEITLIRGDARALPLASGAVDFVLSSQMLHHFSEHDIINLLRGWSRVARRAIIV
                     CDLVRHPVAYHGIRLVTKVLTRNVMTRTDGPLSVQRAFTLEEWRDLFERAGIGPFRL"
BASE COUNT         3531 a         4123 c         4119 g         3292 t
ORIGIN      
        1 tctcgacggc aaccaagttt cgctgtcgtc gttcaggcag aagaagcatg tgcttctcga
       61 gttcggcagc atcacctcac cgtttgtgga aagtttcctc cactaacgaa actttaccgg
      121 agatatcgaa atcagggctt ccagtttgtc ataatctatt cacgcgaagc gcatcccggc
      181 gagcattatc cgcatcacca atcgttcgag cagaaagccg ctcatgcgcg aaaaatgcac
      241 gagctcgaca gggttgacga tattcccatc ctgctcgatg acatccatgg ctcaacgcat
      301 tcggcttatg gcttgttgcg caacagagtt tacctgattg cccgtgacgg cacggtagct
      361 tacaagtcgg actggaccga tgcccacgag atcgacggca tgtgcgccag catccttcga
      421 ttggacgaga tgaaggcgag aaaggtgccg gtcatccgga aaggagtttc agagagattg
      481 cactggatag cgatggatcc cgtgcagcgg gaacaggttt accggcgctc gggaaacaaa
      541 gccatccagg actacttcaa ggcgaaggga agcttgccct atgcaacgga tgcgaagaaa
      601 gggccctcat aagttcgtca cgaccgagta attagcagcc ataagtaact ggcgttcccc
      661 gtcatagaaa agtccgggat cgaattgcgg cagttactac cctgcgattg agtcgcttgg
      721 aacgtgagtt cacgcaagca caaacaatat actcgaactc tatctttccc tgctagttaa
      781 ttgataggct gggtcatggt ccgagttcgt caacgcgatc tctggttctg cctggctttg
      841 atcgcagccg gggtactttt atttaaggca atgcctaccc aatggcagct cgatgagtat
      901 ctttctttcg ttacccccaa gatcggctac agcgacgttg aacgacacta ccatcggcca
      961 tttcaatccg agctagcatc agcgtggaca cgtaggagca ggaccgttgt agaattttcc
     1021 tacatcaagt ttgatcgcaa acaaaatact cgcctctggc ctttcatctt cactggaatc
     1081 acacgatcac cgccggcttc tcgttagccg atagaattca gctggtgcag attgcgaccc
     1141 actgcgctta tcgccaggga tgtgggttgt cgtctgcttt tggttcgagc tcatacctgc
     1201 gagatcgctc ggatcgagaa gaatacgtct acaacggcta cgttcaacaa aggagctgag
     1261 aaatggttcg agtactaacc tacagtgtgc tgctgatagt gggaatggtg ctatcgcagt
     1321 tgcccgccat cgatcatttg aagcccgaaa ttgcggcgct cacaatgatc tttctcgcct
     1381 acataatgat tcaagtcgga atggaatttg aaatcgacaa aaatgacctg cgaaaatatg
     1441 gtgttgatta cctcgtcgct atggctgcgg cggcagtgcc gtggctcacg gctgcggcat
     1501 atttctggtg tttcttccac atcgggaccg ccgaaagcct gttgatcggc cgctttgccg
     1561 ctccgacctc tgccggaata ctcttcacga tgctcgccgc agcaggcctt gctgcaacgt
     1621 ggacctataa gaaggcacga gttttagcga tcttcgacga tctcgatacc attttgctga
     1681 tgattccact gaagatgatg cttcttggat ttacactcaa gctggtcggg cttggcgtcg
     1741 tggttattgt cttgttgttg gcggcttatt ttatgatcca ttggctgcga gttccgacaa
     1801 acaacttttg ggttgccggt tatgccgcac ttgtgtgggg ctcgtgcgtg gcattcgatt
     1861 atgtcaccga gttgcacctg gaagtgttgc tgccggcttt cgcgttgggg tgcctcatca
     1921 agtcggatca tttgcatggt tccgaagagt ttgatccgca cgcaaggggc cccgagcctt
     1981 gcaatcggga gcgggaagac gtgctcgatc gctttatcaa agggtccttc atgttacttg
     2041 ccggcatggc gcttccgccg attcagttcg gtgcaatcgg catcggcatc gtcgttgttc
     2101 atgtgctcgt tttgactttc ctgatgaaca tcggcaaatg cgttccgcta gcggcttacg
     2161 gcgaccaggc cagtttacga gagcgggcgg cacttagtat cggcatgttt cctcgcggag
     2221 aggtaggtat cggcgtgttg ctggtttcat tagagatctt tcaccaaacg ggttctgggg
     2281 acattccagg catcagcgaa agcatttctt tgggtgggct cagcctggcg ttgaatctct
     2341 gtctaaccgg ccttttcata ttggctgtga tacggctact taattcggcc acggctaacg
     2401 actgacaaag ctccagttag cttcgggcga gaaagcgata aaccgattac gttaagccga
     2461 gggggagttc gctcggcaag cgatgcgaag aaaggttcta accctaatcc gacattattc
     2521 taactccgcg aggccagcga gccctacagt cgtgggcaac gggactgtgc cacgaagacc
     2581 ggcccgcatt ccccaagggt gatgtttcgt aagtcgaggc cttagagtca actgcgcgtt
     2641 cgggtgcccc acggcttccg gtctcgctgt cctcgctcac tgctgacggt gccaactatt
     2701 gttagggcta atcagtacgc acatcgatga tccgcactaa atgacgcaag atctttcgga
     2761 acaagaattg tattctttca gagccaccga cgtacccgag acttatagtc aacgaattct
     2821 tggccgaact ttccagccag cgctttttcc tcaggttcga tttggaaccg gttcatgtaa
     2881 caaatgaaaa cagggagaaa aataaacgcc agcgcattcg ataagaaaac tgcccagccg
     2941 accaggacta aaagaaagcc tagatacatc ggattgcggc tgaacttgta aatgcccgaa
     3001 acgaccaaag acgatgacga ttccggcttc attggattga ttgtggtttt agctcgcctg
     3061 aaggacgcaa cgccgacgat gataatcatt gcgccggtga tcgcaaaact caacgccaac
     3121 gatttgcgcc cggggaatac gaagctaagc gccggcatag cccgtgagac aagccacatg
     3181 agcgcagcca gcaacacgcc gacagccaaa ggcggtacct tcaatacgag aacacgcatg
     3241 gcgacggttt aagccccctc ttggtttttc tttgatggga taaccaaacg gtagccgatt
     3301 ccgctctcag ttcgtaacag cttctcgcct cgctccaagc tgtcgatttt cttgcgcaga
     3361 tgcgtcatgt aaacgcgcaa atactgcgac tgctcttcgg cattgggacc ccagacttca
     3421 cgcagcaatt gcttgtggga aacgatttta ccggcgtgct gcgcaagaat tttcagcagg
     3481 gcatactcgg tgggagtgag cttaatttcg tgtcccttca gagtgatggc atggttggta
     3541 aaatcgatgg cgagatctcc agcgtgaaaa aacggctcat ccgccgctga attagaacgc
     3601 cgctggatgg ctcgtaagcg cgccaggagt tccagcgctt cgaacggctt ggtcacataa
     3661 tcgtccgccc cggcatccaa gccggcaact ttatcttcgg ggccgtcccg gacggataga
     3721 atcaaaacgg gcacctggct ccactcgcgc agccgcttca aaacggtgat gccttctata
     3781 tccggaagcc caagatcgag taacacaaca tccggtcttc tgagcgcgac gtcggcaagt
     3841 ccctgtttac cgttttccga ctcaagcact cgatactgat cttcttccaa cacaatgcga
     3901 agaaactttc ggatctgctt ctcatcgtcg atcaccaagg ctgttgcatg atcactcatg
     3961 ccaagctctc catgatttct ggccgaagga tctccaccgg aatgctgatg acgaattctg
     4021 ctccaccgct gctgccgttg cctgcgcgca cggtgccgcc atgcgcccgg acaaaaccgc
     4081 caacgatcgc gagaccgagt cccgtcccgc caggagcggc gtcaacgccg cggtagaacg
     4141 catcgaaaat gcgcagtaga tcgtcttccc gaattccttt tccctgatcc agtaccgaaa
     4201 gaagcagttg ctcctgtgcc agatgagcgc cgattgtgat gttcgtccca ggcgggctgt
     4261 ttgcgccggc gttgagcatc agattgcata ggcattgctc caaaagaggc tgatcgattt
     4321 tgaccatcgg caagcgggta tccacatcaa cgatgatgtg atgctcatgg agcgattcac
     4381 cggcgagctc gatagccgct tgaatgagct cctccacatc gcaccaatcc agcttcggct
     4441 ggatcactcc cgattcaatt cgggtcatat caagcaaatt gttgatgaca cggtgaagac
     4501 gccggacggc tacttggacc tcccgcaaag ttctttgttt tttttcatcg ccatttgttt
     4561 gccgcgtgat tgcatcgatg ccggcctgaa cggcggataa cggtgtcttc agttcgtgcg
     4621 aaacgctttg cagaagcgca cgcctgaggt tctccgatgc ttcgaggacc tccgcgcgct
     4681 tgaacgcttc aacgatgtgg tttttctcca gcaccagacc gataagcacc gcgaaagctt
     4741 cgagtagctc cctctcggcc cggtcccaag gcttagcggc gggcggccga accgcaagca
     4801 cgcccatgac tgcagtgcgc ccctgcaacg gcatatgcag cgccgcggca tccggaaagt
     4861 acgcggtgaa cttcccggcc ggcacccgat ggctgaagac ccatgcggca acgccgttct
     4921 cccggtctga aaggtcaaat gagctcactg catgaggttc ggccgagagt gaatgatttt
     4981 tttgtcggac gaaaagcgct gccttagcag caaaaatcga gtcaatgaga ttgaccgcgg
     5041 ccgcaagccc ggtgtctaaa tccgttgcaa atgcagcctg ctgggcaagt tcatagagag
     5101 cagcggtgcg acgttcccgc tgccgttcga gcatctcgct ccgccgcagc cggctggtca
     5161 gatgccccat agctaaggcg acgacaaaaa aaaccaggaa cataatcccg tcgtggtatc
     5221 tctgaacatg aaaagtgaag tacggcggca cgaaaaggaa gttccataag acggcgctga
     5281 atgcggccat tgttagaacc gggccgcggc tcaacttcag tcccagaaga acgacgagaa
     5341 ggagataaag tagggcgaca gcttgatagc ccgcgaacgg ctcgatcaac caagcagccg
     5401 cggtcaatgc taggatgagt gcgccggcta aagaaaattc gttaacgctg gaggcagaag
     5461 ggatccaggc gggtaatgaa ttcaagcgga gacggcgtct tcggggtcga accagttccg
     5521 aatcgcgtga gcctatcacc atttggctga agtatatccg gtttggtcta tgggatgtaa
     5581 ggactcggac agcgacacgt actcccaata aggaactaga ataccgtcca tttcagccac
     5641 aagccggcag ctcacgcgct acttcgccgc ccgccaacga agcaaaaacc cggacaggca
     5701 ggaccatcat ttcgtggccc tcgaactgaa ggcgctgttc cagagtgaat ggcgtatggt
     5761 tatgcagcag gcgtgtcaaa aagttctcct cttgaaacac cagtttccct gcaaagaaca
     5821 ccgatccggg aaactcgctg gctacagact tgcagagttt ctccaactca tccatgaggt
     5881 ctattcccaa cccgtaacga tactcggcgt accagcccaa gcagttggca aaatccacga
     5941 agcttttcag atcctctact ttcatcctgc ttaagctctc tatctcgtta aggcccttga
     6001 atcgtcccga gtcgatcact gcgattgaga cgaaaacgat gttcttgaag tgattcggga
     6061 aaagccggcc gatactaagg agcgaatgaa ccgccaggcc gtcgaagtcc cttacgatca
     6121 aaaccgcagt gggaccatcc gggctctttg ccggcaccgc cttcatctcc ggctcgaacg
     6181 ggatgttcat taacgcggcg tcgagccgct ttagcgcgcg gcgtacatga tcgtaatgcc
     6241 gtcgaaccca gtaacagacg acaatgaagc ccgctgtcac tagaacggtg acccaacctc
     6301 cttcggcgaa ctttagaatc accgtaatga tgagaatggt acccgtgaac gataaaccga
     6361 ggccattcac aaacaacctt cgaagccaaa gcttatcggt gtgtctcact cgccaccagt
     6421 ggcggcacat tccgagctgc gaaatgctaa aagtcaaaaa gacattgatc gcatacatca
     6481 ccaccagcac tcttacgtcc ccctcgctat aagcgagaaa agccaaggag gccagaccca
     6541 tgaaccagat gccgtatcgg gtaaccaagt gatcgctcag atgagcaaac cgatgaggca
     6601 cccaagagtc gacggccata ttggaaagca cccgcggtcc atcgatgtaa ccagcctgcg
     6661 ctgcgacgat gaggatggcg gcttcggtga cgagggtgac gatgacgaga acataggcta
     6721 aatccggtga aaagaaacct ccgtagattt ttgcaaacag actggcgttt agagttttgc
     6781 cggtttcatg ggagacctgg ttcaatagat aacagagcag caaaccgctg gcggtgaaag
     6841 cgagcgatgc cgccatatac agcatggttt ttttgccggt cgcaactcgc ggttctcgta
     6901 aaacctgcaa tccattgctg acggcttcga tgccggtata ggtgccgccg cccaggctat
     6961 aggcgtgcag cacgatgaat agagtcgccc acaagcctaa gccttggata tcgccgaccg
     7021 tattttgata ggtagtcgac gctagcagcg gaatctcagc tgcctgaggc aggatgccga
     7081 ggccaattgc gaagatgtgc gcaacgacaa aaagaagaaa gattggcagc aaaaagagga
     7141 ctgactcttt ggtgccgcga aggttaagat agatcaaaaa aaagatcagg ccgaattcta
     7201 ctgccaattt agacccctca ggcgaaatgg gcaggaagct gaaaatctga tcgcccgcgc
     7261 tggctacgga aatagtgatc gtcagcatgt agtccacaat caaggcggag ccggagacca
     7321 agccgagctt ggggcccaac agttttgtcg cgaccaggta accgcctccg cccgtcggaa
     7381 aaagagcgat gatctgacta tagcttgcgg atatgacaaa gaccgtcagc gccatcagga
     7441 gcgccagcgg cagcgcaaga aagaaatgat cgccaagagc cagataggct tcttcagggc
     7501 cgtacgctga cgaacttaga ccgtcggcac ccagacccac ccaggcaaga aaggcgacca
     7561 gcgatacctg atgaaacact tgcgggtcca gaggatcctt gggcttgccc aatagtaaac
     7621 tcttaatcga catcgattta gcgatcctgg tttgagaact taactgtgaa gtaccggacg
     7681 aacggatttc atagggttgt aggggatgac caagactgga cactgagcat gagcgacgac
     7741 attgcaggcg acgctgcgac cgcggaatag tctgaaactt ctgccccact ctcgggttcc
     7801 gatcacgagg agatcgacat tttccagact cgcggcgcga acaatctgct taccaatgga
     7861 atctgtgcca tccaataatg aggctctcgc ccttactccg cttgcattca cttgtgcaac
     7921 gagccgggat aaggccatct ctgcttccgg cttctcgaag gactcagctt caaacaacgg
     7981 tatcggaggt gggagaacgt gaagaagcaa gagatccgca tcactggccc tggtgatctg
     8041 gatcgcccag tgaagagcgc cgtcagaaga gttgctcaga ctcgtaggaa atgcgattcg
     8101 cctgagtggc cctgccaacg gcctattcaa tgccgaggga actgtatgct ggttaagcga
     8161 caacgtgccc atgtcgtcac tgtaccggga gtgggaaacg ccgactaata aaaatcgtgg
     8221 ctcaacaggt taaaaaaatg tttaaacggc atccgtcatc ttgcttcatc atgaggggtg
     8281 ccggtcgaca aactagccgt tatccttcga tttagaggcc gtagaatcgg caacgtgaga
     8341 ggttggaact atgcaaggct gaaagtacct aattgacaat catcaaccga caatcatggg
     8401 gtagcttctt catcagacct ttgactacat gaccgcgcag catgtgataa aaagccgacc
     8461 gtcgcgacgc gccaatgacg acggcattga cgtcgagtgc cttggctgca ttggcaatcg
     8521 cctcggcagc gttgtgcgac accgcccaaa tcggcacgat ctcgatctga ttttcacggc
     8581 cggcttgcaa cgccgccttg agagtttgcg ttccctgctc gttaggcatg tgcggcgtgt
     8641 ctcccgcgaa tagtcccggc cactcctcga cataaataca attcaaagcg ggctcgcccc
     8701 ctcctctggc gcgtacgatc gcctctttga tcaagcgtgg gttctcatcg cgcagcgcaa
     8761 ggagcgtcga tgaagagtaa agcggcttta cttcaaccgc ttccgtcaag gtcatgagcc
     8821 cttttaattc gtcttcgaca agatcttcgg aagcgcggta tgctctagct tgcagccgtt
     8881 ggacaaacgg tatctgaagc agcaaatcga caaaccaggc gcggcgcacg cccacggcaa
     8941 tgagcatacc aatggtcgta actgctccgc cgaaataagt cgcccgctct ttctcgacaa
     9001 ggttcactgc ccaggccacc aggaccatgc ccgtggtcag gactcctaac cagaatcccc
     9061 aatcacgcct tcccagcttc cagcggatcg agtccaggct cagtgatgaa aatacaaacg
     9121 cgcctagtag accgaacgca tacatatctc ccaatatgtc gagctcccct ccgctgaaga
     9181 aaatcaccaa ggccgggacg atagtggcaa tgccaacggc aatgtgcggt gtgttgaagg
     9241 taggattacg aacagcgatg atttgcggca ggaacccgcc ctttacgagc gccagaaaaa
     9301 catgatacga gccgatgatc gcggtattcg cggcaaagag cagcagactg gaggccgtca
     9361 aaacaacagc caatctgatg ccgtagccgc ccgcgatcag tcctagctca gagatgaatc
     9421 tctcactctc gcttgccttg aactgttctg gtaacaagcc gatcgataat gctgtaagca
     9481 ccggcgctgt cattaccatg gtgacgatta ccgcgcccat cgcccagcgg gtggtttggc
     9541 gcagcggcag gcgcatagcc ggactaagct ggctaatgct ttcgagtccg gagaacgcga
     9601 gccaggcgga agaaaacccg acaagcagct cgtacctcga tagttccttg ccggtgccca
     9661 atctgcttaa caccgcttgc cactgtgatg catccatgcc gataaacgct gcgccgatga
     9721 ccactagatt caccaggaac gaagcacaag ccatcaccag cgccaccgtg gcgctctcgc
     9781 ggatgccgac gatattaagc gccgccagga cgcccactcc gaggcaggcg agcgccacga
     9841 tatgtgagtc gattcccgaa aaaatgctgc cgacatactg gaatccggaa acagcggaaa
     9901 ttgcagtcgt caggaagtaa tcaaccgtga tgagcatccc gcccaggcat ccccacatcg
     9961 ggccaaaagc tttggtagta atcgtgacga caccaccacc ttccggattg cgccacgaga
    10021 tctcgatgta tttcaatgcc agcagaatga aaccgccggt ggttagcaaa acaaagaacg
    10081 gggtcgcgtc tttgacatgg ccatagagga ttccgggcac gtaataaacc gacgtgccga
    10141 tatcgcagaa gacgacggcc caggcgagaa accagttgag ctgccgggtg tttttactcg
    10201 gttgcataat cggccaagag gatttgcctg cagcggctcg gcctatcgat caggtccgcg
    10261 ccgagctcaa actcatgttc acttgcaaaa ccgtttgatt ccgcggtgcg ttaagaattg
    10321 cgccgctttc cggatccgcg cgtggataac tcacagcttg gccatgctat agcctatccg
    10381 gagggttaag gccgcgctaa ttaaaatact ggctcaaggt gtaaaaaaaa cgttaagagt
    10441 gaaacccgag ccgcaacaag gtagcggctc aaccagcacg gccggccatg gctcagttcc
    10501 ggaaagaatt ggagggcatg tgaggaaaag cggattgttt ctgttgttga tcacatcgac
    10561 gttcattgga tcttccgatt acgcgccggc gcaggtgcga tcaagcgcgg agaaggtccg
    10621 ggtggtttac agcgccatcg gcagctcgca aagccctttg tggattgccc atgaagcggg
    10681 cttatttaag aagcatggcc tggatgccga gattctctac gttgccggcg gctcgcgagc
    10741 ggcgcaggtg atcttgtccg gcgaggcgcc gattgccatg ttcaacggca gctcggtgat
    10801 cagtgcaaat ctcgccgggg ccgatctggt taatctggcc agcggcatga atgtgctgcc
    10861 tttcttcctg gtcaccggac ccgggatcaa acagattgag gatctaaaag gcggaaaggt
    10921 tgcgatcaca cgatttgctt cggcaaccga cttcgctctg cgttttgccg cggaaaagtg
    10981 gccgctcaaa ccggagcgcg attttgcggt gatacagtta ggcgggcagc ccgaaatgat
    11041 ggccgcgctt aagagcggcg cggtgcaagg agccatgctc aacgcggagt tcacgattct
    11101 cgcgcgtcgg gaaggctatc gcgatcttgt ggacgtagct tcgctgggcc tggtttttcc
    11161 gggttcggga gtcaacacca gccggtcttt catcaagacc cgccgcgata ccgtgttgcg
    11221 agtcatgcgc gcttataccg aaagcgttca cctcggcaga accaacaaga gctttggagt
    11281 taaagtgctt ggcaaatatc tcaagaacca ggacgtgccg tttttagaag cggttcgcga
    11341 attgtatctg ggtcactata ttccttggcc tccctaccct tcagtcgaag ccatgaaacg
    11401 ggcgctcgac gatgtcgccg aaagagatcc ccgcgcccga ggcatgcgcc ccgaacagtt
    11461 catcgatgcc accctaatgc aggagctcga caaggagggg tttatcaagc aactggcgcg
    11521 gtgaaactta caacaagatt ctgttcaagg ttcaacgttc aacgagggac agggctgatc
    11581 aaacgagaaa agtcgtcatc tcacccggcg catttacttg agttttttca gctcttgcag
    11641 aatttgtttg ttcgaagctc ggatggtgta atttgtttca acgaatttcc agcgcacgac
    11701 gccttggcga tcaatcacgt aagtagcggg gtgaggccag cctttgccgt ccgggttgag
    11761 aagcccatag cgatcgatca ctttgtgatc ggtatcttga agcaagggaa aggtttggct
    11821 gccattgttt actgcttgaa gtctttgccg cagctttttc gaatcctcat gactatcgat
    11881 gcttaccgcc aggatttgcg attgcgcact gtcttgtttg cttagcagag tttgcagctc
    11941 gccgagctgc gcgacacagt agggtcacca gtggccgcga taaaaaatta atacgacatt
    12001 ctttttgccg cggtaatcgg caaggctgac gttcttgcct tcggaatcct cgagggtaaa
    12061 atccggcgcg ggctgcccaa ccttgacccg ttcgagatcc gttgctaacg ggttgcttcc
    12121 atccttcgca gcggtttggg cggaaaccgt agcttggatc ggcaggatca taagagcgaa
    12181 cagcaaaatc aatgctttca tcaggattct ttgcctcctc cgaaaaacgt agatcgaccg
    12241 gtccgattat gtcacagctt tttcgcgagg caaaacatga gaacgaaaaa gccggtacga
    12301 aatagcgcga aataatcccg gcatgaagcg cttgatggcg cacatcaacg cgaacgagcg
    12361 cggcgttatc atttcaaagc gcttattctc gatgattgtc atcacccttc gagagaccga
    12421 ctcaggggtg atgatcatag tttccggcga cttcggcgaa gtttgcgcga agctccggtt
    12481 gaatgcggtt ttcaccggac ccgggctaac caccgaaacc gcgatgccgc gcggcctgag
    12541 ctcgtgataa agactttcac tgaaaccatt gatcgcgaac ttggaagcgc aataacccgc
    12601 catattgagg gtgccaatgt gcccggctac ggaggagata ttaatgatat gaccacgccc
    12661 cctggcaatc atcgagggca atacctcgta ggtgcagtaa actgtgccga ggtagtttgt
    12721 gcgcatgagc tcttcgatta tgtcgagcgg agtttcaagg aacggcttgc gcatgccgat
    12781 gccggcattg ttgatgagaa tgtcgatagc tgagaattgt ccgaacactt gactaatcat
    12841 ttgctgaact tctgtccggg agccgacgtc acagcggatg gcgatggagc gcgggctggc
    12901 gcgccggagc tcagttagaa cctcctcgag ccgagccggc gatcgcccgc tcgcaataat
    12961 gaccgcacca cgcgctgcta ggtccagtgc taggcgtttg ccgataccgt ccgatgcgcc
    13021 ggtaatcaaa atgacgcgat ccgtaaagtt catccgacgt ttctaggtta gctcacccgg
    13081 taatgttatg ctgaaaggaa actatgtcgg acataaagag tcaatatgag ctccgataac
    13141 ggtcacacgt acaacggggg cccggcgcgg cgccgtgtag tggttaccgg aattggcgcc
    13201 attagcccga acggcattgg ccgtgaggcc tactggaagt cgcttcgttg cggccgcagt
    13261 ggcgtcgatt tgatttcgca gtttccgact gacgagctct cgtgccaaat cgccggtgaa
    13321 gtaaaaaact ttcgcgccga ggattacttg ccgccgcaag atttgcgccg cgtcgggcga
    13381 gcggtgccgc tgctgctcgc cgccaccgcc gaagccttga ctgatgccgg tatcgtttgg
    13441 cagcagttga gcctcgatga aagacgatcc tggggtgtca ttgtcggcag cggcggtggc
    13501 acgccggatt tcaccgaaga gcagtaccgc ctgttttttt ccaaccagat gcgcaaggtg
    13561 agcgcctaca atgtctcggg ttcaaccccc ggcaatgtct ccagcgaggt ctcgctccgt
    13621 ttcgacctgc gcggcccgag tcatctcatc tccacgggct gcaccagctc cactgacgcg
    13681 ctcggctatg cattcaacat gattcggttc ggtttggccg agcacctggt cacgggcggc
    13741 accgatgcca caatcacgcc gggaatcatg cagggctttt gcgtgatgcg agtcgtctcc
    13801 tgttcacgca acaacgagcc gcgctgtgct tcgcgcccgt tcgatcgcag ccgcgacggt
    13861 ttcgtgctcg gcgaaggcgc ttggaccttg gtcttcgaag agcgccgcgc cgctctcgcc
    13921 cgcggagcaa aaatttatgc ggaagttctc ggttatggct cgacctgtga tgcttatcac
    13981 cgggtgcgcc tcgatcccaa tggtgaagag ccggcacgag ctatgagctt ggcgatccaa
    14041 gacgctggca ttgccaagga cgagattggt tacctggcac tgcacggtac ctcgacaatt
    14101 ctcaacgaca ggaccgaaac cgtggccgcc aagctttgtt ttggccggcg cgcttacgag
    14161 attcccatga gctccgtgaa atccatgatt ggccatccgc agggcgcgtc cggcgcagcc
    14221 ggcgtggttg ccgcaatcat ggcgatgaat cacaaatttc tgccgccgac catcaactgc
    14281 gacgagccgg atccggagtg tgaccttaac tatattccca atcggggcgt ggcccgcgcc
    14341 gtcgatattt cgctgtgcaa ttgtatcggc tttggctcga agaactcagc cttggtaatc
    14401 gctcggggag agatgtgaag cgcagcatgg cgcgtgagat gatggactcg ccggacaata
    14461 gcccgcgcga gcttctggaa gatgatctgc gcaatttgcg aatcatcaat cgatatctcg
    14521 gcaaccaccg agtcgtgctt ggcggtctgg cgcggctaat cgaggagcaa aagttaaagc
    14581 ggttttcgtt gctggatgtc ggcacgggca gcgccgatat cccggccctc attgcctcat
    14641 gggcacggcg ttatcatctc atcgctcggc tgatcggtct tgagccggaa ccggtgaccc
    14701 tgcgagcagc ggccagccag attcgtgatt attcggagat cacgctgata cgaggcgatg
    14761 cccgggcgct gccgttggcg tcgggtgccg tggatttcgt gctctcatcg cagatgcttc
    14821 atcacttttc cgaacatgac atcatcaatt tgctcagggg ctggtcccgt gtggcgcgcc
    14881 gggcgattat cgtttgcgat ctggtgcgcc atccggtggc gtaccatggc attcgtctgg
    14941 tcaccaaggt cttgacccgc aacgttatga cccgcaccga tgggccgctc tccgtgcaac
    15001 gggcatttac cttggaagaa tggcgcgact tgttcgagcg tgccggaatc ggtccgtttc
    15061 gactg
//
LOCUS       DASUVS010000336         8979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1888862, whole genome shotgun sequence.
ACCESSION   DASUVS010000336 DASUVS010000000
VERSION     DASUVS010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8979
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1888862"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1574)
                     /locus_tag="VF182_18015"
     CDS             complement(84..1574)
                     /locus_tag="VF182_18015"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6439034.1"
                     /translation="MTALEARKLAEDEIKAGKTPKYTDLRDWLEIVDSFGELKKIDGA
                     DWNLEMGTLAELLSRETKSGAVPAVLFDNVPGYPKGFRTLFAQNASFKRMALNLGLPL
                     ELSNLDLVRAFRQKLVAHEPIKHKVVTKGPILENVDSGDKVNLLKFPVPFMHELDGGR
                     FIGTACLVTTRDPDEGWVNFGAYRGMVHDEKSMGLYISPGKHGSIQRAKYFERGKPCP
                     VVISVGQDPVLFMVSGNEIDYGVGEFDYAGGLKGAPIEIIEGKATGLPFAANAEIVIE
                     GWMDPTDRKTEGPFGEWTGYYASSSRPEPVVRVENIYYRNDPILCCARPGRPPSDYSI
                     AKCMVKAALIWEQVEKCGVPDVKGVWCHEAGGGRLFNIISIKQRYPGHARQAMLAASQ
                     VHAGAYLGRYVIVVDDDIDPTNTFDVIWALASRSDPVESIEILRRCWSGPLDPRIHPS
                     KKGFNSRAVIDACRPFEWMKEFPPVAESSPELREKIYNKWKKVIEA"
     gene            complement(1574..2758)
                     /locus_tag="VF182_18020"
     CDS             complement(1574..2758)
                     /locus_tag="VF182_18020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6439035.1"
                     /translation="MADKRDMVQIEALEKYFGEDKERVHVLKGVSLNIPEGLLYTFLG
                     PSGCGKTTTLRCVAGLERPDGGKISIGGQTVFASGERVYVPTNKRPIGMVFQSYAIWP
                     HMTVAQNVAYPLTIQRRSKDEIRRKVKDVLRIVGLDGLEDRPAPKLSGGQQQRVAFAR
                     ALINEPKVMLLDEPLSNLDAKLRVQMRSEIKALQRRTNITTIFVTHDQAEALAISDQI
                     AVMHGGTLIEVGSPHQLYTRPKRRFTATFLGLTNLINGKVLDVDGDGRPGKMETKKGI
                     LSFIPAPGLKKDQAAVISIRPENIPLHKEKPQSLENVLEGTVAEAIFMGDSYQCKIAV
                     GEDILAVHTHPFQSVNPGDKVYLHLDPKSCNGLPADDTEGVEETMVANYDKESPQAKG
                     VL"
     gene            complement(2816..4507)
                     /locus_tag="VF182_18025"
     CDS             complement(2816..4507)
                     /locus_tag="VF182_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017131336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6439036.1"
                     /translation="MRRPNSQTLILIGAIIFVVYLAGVPLLMLLYGSIRSAPIGEPGA
                     TYTIQNYVRAYFDKEFYLLFLNSLYFAVGTCALTCLIGTFLAWVSERTNTPFKKVFVV
                     MALVPFIIPGILSTISWILLLSPKIGLINLTLKGLLGLESAPFNVYSMWGMIWAEAIH
                     DYPLVFLLMSAALRNMDTSLEEAALTAGSSTWTTFKRITLPLMRPALFSVLLIVFIRG
                     IEAFEVPALIGVPAKISVFTTKIFLAIHQFPSDFGLAGSYAVTLLVISSIGVLIYGRI
                     TRREERYATVTGKGYRPRVIDLGKWKYFTCALSFFIFFLAVVLPVFVLLWSSFIPYYG
                     VPSLELMGKMTMANYHYILNYPLAVTAFKNSFYLSVGSATLVMLLTSVIAWVTVKTRL
                     PGRALLDNMTFIPIAMPGIVLGVSLIWVYLTLPIPIYGTMWVLLLAYITKFMPYGIRA
                     ASASMIQINKELEEASLTAGGTWLQTFKKIILPLLMPGFTAGWIYISIIALRELSTSI
                     LLYSYNSTVLSIMAFDLWEGGQYTYVCALGVLMVLLLLVMAFIARKLGAKMGIAQ"
     gene            complement(4530..5345)
                     /locus_tag="VF182_18030"
     CDS             complement(4530..5345)
                     /locus_tag="VF182_18030"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6439037.1"
                     /translation="MAQTKLKAVNLRMEYYQPRTGGRLLALDNVNLQVDEGEFVTIVG
                     PSGCGKTTFINLADGLLKPTGGQLMIDGQPVTGPGTDRGMVFQDSCLMPWRTVFKNVI
                     FGLECQGLDNSEGKDRAQKFIKLVGLAGFEDHYPHELSGGMQQRCNLARALTVDPQIL
                     IMDEPFAALDAQTREIMQSELLRIWRESGKTVLFITHQINEAIYLADRVIVFGARPGR
                     VKEMMKIDIPRPRPLAVKRDKRFLEYEDHLWTMIEEEVKKTMVQDQVVHNIDA"
     gene            5570..6337
                     /locus_tag="VF182_18035"
     CDS             5570..6337
                     /locus_tag="VF182_18035"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX6439038.1"
                     /translation="MDDPLKQSKTKARGTENLSARVYNEIKNLILCNEVMPGQKLHHQ
                     QLSERLGVSRTPVREALTRLVQEGYVAFLPNRGFTCKEIRMQEAEELYELREALEAFA
                     VQKAIENLTDTGLDKLRYKMELYGSDVQNRFTRERLMYDQDIHLEIARLAGNETLEKS
                     LGHVFERIVLKRRTDGLYDAARGVNAHQQHLRLLRAMEQRNVTEAVSIVRAHIRKGKQ
                     NVLSDLEQRLAIRKRATATVEEREKQTYRKEEKWPQI"
     gene            6322..7692
                     /locus_tag="VF182_18040"
     CDS             6322..7692
                     /locus_tag="VF182_18040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HEX6439039.1"
                     /translation="MAADLVIKNGWVVTPEETFQGGVAISNEKIVAIGANDVLPQGKE
                     EIDAKGKHILPGIIDGHVHFREPGMTHKEDFTTGSTAAVCGGITSVVDMPNTVPPTAQ
                     AEQVKIKQQLGEAKSLVDFGVTGVVVQTNTKDILPMADAGAIGFKIFFGETIGNLPFP
                     DDGMCMEAFDYIAQSKLPLGIHAENRQIMAYCTNKLKSDGHNEARYWEASRPDICEAE
                     SVHHAIFFAETFGTKLHVFHMSSKQAAYMVRDAKARGMRITAETGPHYLLREPNDMDQ
                     VGSLLKMNPPVRTKDHGEVLWQGLREGFVDMLATDHSPHTLEEKGSDIYGKLTKPAIW
                     DCISGFCGVETGVPLILTEVNKGRLTLNQYVKVACENPAKVWQVYPKKGAIRLGSDGD
                     ITIVDMDKEGTIDVNKLHSKNKPSPWHGWKVKGMPVCTVVRGHVQMRDGEPCGKPIGR
                     MLRPLI"
     gene            7830..8741
                     /locus_tag="VF182_18045"
     CDS             7830..8741
                     /locus_tag="VF182_18045"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6439040.1"
                     /translation="MKRTEAFEMYQPSTLREASLLLKEKGPGGRFLAGGTDLVIAMKE
                     KGLVPKYVVDLKRLPGLSGIRENSDGSITIGALTTMREIEISPLLTKKYPFLCQSAAE
                     VGSIQIRNRATIGGNMSNATPSADVAPSLIALQATAKIACARGERSVAIEEFFRGPGL
                     NTMDSDEILTEITIPKTSPRLVGDYIKFSPREMMDLAYVGIAVAYILGEKENRCSQVR
                     IVLGAVAPTPIRAKKAEAALEGQVLSEDLAEKVGAIAAEEARPISDVRSSADYRRAMV
                     GAMTKRALLNATVGPARSWVERRDRRY"
BASE COUNT         2270 a         2372 c         2344 g         1993 t
ORIGIN      
        1 atctatctgt tgcaacagaa ttcaaaaaaa gagggcgact gcatctgcgg ccgccctcgg
       61 tgtaagcaca accagagttt ttcctaagct tcgataactt tcttccactt gttataaatc
      121 ttctcgcgca attcgggaga tgactctgcc accggcggga actctttcat ccattcaaac
      181 ggccggcacg catcgatgac cgcccgagag ttgaaaccct ttttgctcgg atggatgcgc
      241 ggatcgagag gaccgctcca gcaacggcgt aggatctcga tagactccac cggatccgaa
      301 cggctcgcca aagcccaaat aacatcgaac gtatttgttg gatcgatgtc gtcgtcgacg
      361 acgatcacat agcggccgag ataagcgccg gcatggacct gcgacgctgc cagcatggct
      421 tgacgcgcgt ggcccggata acgttgcttg atcgaaatga tgttgaacaa acggccgccg
      481 ccggcctcgt ggcaccatac acccttgaca tcgggcacgc cgcatttctc aacctgttcc
      541 cagatcaatg ccgctttgac catacacttg gcgattgaat aatcagacgg cggccggccg
      601 gggcgcgcgc aacagagaat cggatcattg cggtaataaa tgttttcgac ccggactacc
      661 ggttccggac ggctcgagct ggcataatac ccggtccact ccccaaacgg tccttccgtc
      721 ttgcggtcgg tgggatccat ccatccttcg ataacgattt ctgcattggc ggcaaatggt
      781 aacccggtgg ctttaccttc gatgatttcg atgggagcgc ccttaagccc gccggcataa
      841 tcaaattcgc ccacaccgta atcgatctcg ttacccgaaa ccatgaacaa cacagggtcc
      901 tgacccaccg agataacaac cgggcagggt tttccgcgtt cgaagtattt ggcgcgttga
      961 atactgccat gcttaccggg agaaatgtac aaccccatgc tcttttcgtc atgaaccatg
     1021 ccgcggtacg cgccgaaatt gacccagcct tcatccgggt ctcgcgtggt aaccaggcag
     1081 gcagtgccga tgaagcgtcc gccatcgagc tcgtgcataa acggcacggg gaacttgagg
     1141 agattgacct tgtcacccga atccacattt tcaagaatcg gacccttggt cactacttta
     1201 tgtttgatcg gctcgtgggc aaccagcttc tggcggaagg ctcgtaccaa gtccagattc
     1261 gagagttcca gcggcagccc gagatttaac gccattcttt taaatgaggc attctgggcg
     1321 aagagagtgc gaaatccttt cggatagccg ggcacgttat caaaaagtac cgccggcacc
     1381 gccccacttt tagtctcgcg cgaaagaagc tccgcgagcg tgcccatttc aagattccaa
     1441 tcggcgccgt cgattttttt taactcgccg aaactgtcaa caatctcgag ccagtcacgt
     1501 agatcagtgt atttcggtgt tttaccggct ttgatttcat cttcggctag ttttctagcc
     1561 tctagtgctg tcattacagt actcctttcg cctgtgggga ttctttatcg tagttcgcta
     1621 ccatcgtttc ttcaacaccc tccgtatcat ccgccggcaa tccattgcaa ctcttggggt
     1681 ccaagtgcaa ataaacttta tcgccaggat tcaccgactg aaatgggtgt gtgtgaacag
     1741 cgagaatatc ctcacctacc gcgattttgc attggtagga atcgcccata aaaattgctt
     1801 cggcgacagt gccctccagc acgttttcca agctctgcgg tttctccttg tgcaacggta
     1861 tgttttccgg ccggatcgaa atgaccgcgg cctggtcttt ctttagcccg ggcgcgggaa
     1921 taaagctcaa tatacctttt ttggtttcca tcttgccggg cctgccgtca ccgtcaacat
     1981 caagaacttt tccattgatg aggttggtga gccccagaaa tgttgccgta aatcgccgtt
     2041 tgggtcgagt gtaaagttgg tgcggcgacc cgacctcaat cagcgtcccg ccatgcatga
     2101 cggcgatctg atctgaaatt gccaaagctt cagcttgatc gtgcgttacg aagatcgtgg
     2161 taatattcgt ccgtcgctgc aaagctttga tttccgagcg catctggacg cgcagttttg
     2221 catcgagatt gctgagcggc tcatccagta acataacttt aggctcattg atcagagcac
     2281 gggcgaacgc aacccgctgc tgctgtccgc cggaaagttt cggagcgggg cgatcttcca
     2341 gtccgtcaag tcctacaatc ctgagaacgt cctttacctt gcgcctgatc tcgtcctttg
     2401 atctgcgttg aatcgtcagt gggtacgcga cattttgagc aaccgtcatg tgcggccaaa
     2461 ttgcgtagga ttgaaacacc atgccgatcg gacgtttatt ggtcggaaca taaacgcgtt
     2521 cacccgaagc gaaaaccgtt tgtccgccga tgctgatctt gccaccgtcc ggtctctcta
     2581 acccagcgac acagcgcaag gtggtagtct tgccgcagcc gcttggtccc aaaaacgtat
     2641 agagcaatcc ctccggaatg ttcagcgata cgccctttag aacgtgaacc cgttccttgt
     2701 cctcgccgaa atatttttcg agggcctcga tctgaaccat gtctctctta tctgccatat
     2761 ctcctccgca atgggtgacg gcgatccgga tcgcaaattt aacgacgcga ttgacttact
     2821 gcgcgatccc catcttggct cccagcttac gcgcgataaa tgccatcaca agaagcaaca
     2881 gaaccatcag gactcctaat gcacaaacgt aggtgtattg cccaccttcc cacaagtcaa
     2941 atgccataat ggaaagaacc gtgctgttgt aagaatagag taagatggac gtggaaagct
     3001 cgcgtaatgc aatgatactg atgtaaatcc atcccgcggt aaaaccgggc atcaacagcg
     3061 gcagaatgat tttcttaaag gtttgcaacc aggtgccgcc agccgtgagg gatgcctctt
     3121 cgagttcctt attgatctga atcatggatg ccgatgccgc tcgtattccg tagggcataa
     3181 acttggtgat gtaagctagc aacaataccc acatcgtgcc gtagatcgga atcggcaatg
     3241 tgagatatac ccatatcaag ctgacaccca gtacgatccc cggcatggca atcggaatga
     3301 aggtcatatt gtccaacagc gctcgtcctg gaagcctggt ttttacagtt acccaggcga
     3361 tcaccgaggt gagcagcatc accaaggttg ccgaacccac ggacagataa aaactgttct
     3421 taaaggcagt caccgcaagc ggatagttca agatgtaatg gtaatttgcc attgtcatct
     3481 tgcccatgag ctcaagcgac ggaacgccgt aatacggaat gaacgaggac cacagcagca
     3541 caaacacggg cagaaccacg gctaggaaga atataaaaaa cgacagcgcg caggtaaaat
     3601 atttccactt acccaggtcg attacccgcg gacgataacc cttgccggta acggtggcgt
     3661 aacgctcctc gcgccgtgta atcctgccgt agattaaaac gccaatgctg ctgatgacta
     3721 acagcgtgac cgcgtaggag cctgccagcc cgaagtcgga tggaaactgg tggatggcta
     3781 gaaaaatctt ggttgtgaaa accgaaatct tcgccggcac cccgatgagc gcaggaactt
     3841 caaatgcctc aatgccgcga ataaagacga tcaacaacac actgaatagg gccgggcgca
     3901 tcagcggcag ggtaatccgc ttgaaggtgg tccacgtgct cgacccagca gttaacgccg
     3961 cttcttcgag agaagtgtcc atattacgaa gggcggcaga cattaggaga aacaccagcg
     4021 gatagtcgtg gatcgcctcc gcccagatca taccccacat ggaatagacg ttgaacggcg
     4081 cagattccag cccgaggagt cctttgaggg tcaaattgat caatccgatt ttaggactga
     4141 gaagaagaat ccacgagatc gtgctgagaa tccccgggat aataaagggg accaacgcca
     4201 tcacgacgaa gactttttta aacggagtat tggttcgttc gctgacccac gccaggaacg
     4261 ttccgatcaa acaggtgagc gcgcaggtgc cgacggcaaa gtagagcgaa ttgagaaaaa
     4321 gaaggtaaaa ttccttatcg aaataagccc tgacgtagtt ctgaatcgtg taggtagcac
     4381 cgggttcgcc gatgggagcg cttcttatgc tgccgtaaag cagcataaga agcggaacgc
     4441 cggccaggta aacaacaaaa atgatagcgc cgatcagaat cagggtctgg gagtttggac
     4501 gccgcaccct taactcctga acgctggcgc tatgcgtcga tattgtgcac gacttggtct
     4561 tgaaccatgg ttttcttgac ttcttcctca atcatggtcc aaagatgatc ttcatattcc
     4621 aaaaatcttt tgtctcgttt gacggcgagc ggtcgaggac gcggaatgtc gatcttcatc
     4681 atttccttga ctctgccggg tcgcgcgcca aacacgatca cgcggtcggc caaatagatg
     4741 gcttcgttga tctgatgcgt gataaacagc acggtctttc ccgactcgcg ccagattcgc
     4801 agcaactcgg actgcataat ttctcgagtc tgcgcatcaa gtgcagcaaa aggctcatcc
     4861 atgatgagga tctgcggatc aacggtgagc gcccgagcta gattacagcg ctgctgcata
     4921 ccaccggaaa gttcatgtgg atagtgatct tcgaatccgg caagcccgac caatttgatg
     4981 aacttctggg ccctgtcttt gccttcacta ttatcgaggc cctgacattc caagccgaag
     5041 atcacattct taaaaaccgt tctccacggc atcaggcagg aatcctgaaa aaccattcca
     5101 cgatcggttc cgggcccggt taccggctga ccgtcaatca tcagttgtcc gccggtcggc
     5161 ttgagcaacc catcggcaag attgataaag gtggtcttgc cgcaaccgct aggcccgacg
     5221 atggtgacaa attcaccttc gtcaacctgg agattgacgt tatcaagcgc aagcagccga
     5281 cctccggtgc gcggctggta gtattccatc cgtagattga ctgctttaag tttcgtttgt
     5341 gccacaaggc ctccttaaaa atttcttcct aggaaaacgc tattgaggac tatactttta
     5401 tccaccagca gaccgcaaaa cgtcgtacat ttagcaaaat aaaatctttt ttcaagcgtt
     5461 gtctcgtaag ttttttcaag tgttatccca taagattgac aacggttagg cgattcggta
     5521 gctttcccaa acacaatttg cataaaatat tttatgcaaa ttcaggtcga tggatgatcc
     5581 gctgaagcaa tcaaaaacta aagcgcgcgg cactgaaaat ctttcggctc gcgtctacaa
     5641 cgaaatcaag aatctaatct tgtgcaatga agtcatgccc ggacaaaagc tccatcatca
     5701 gcaactcagc gaacggctcg gcgtcagtag aactcctgtt cgtgaagcgt taactcgact
     5761 agtgcaggaa ggatacgttg cgtttctgcc gaatcgcggt tttacctgca aagaaattag
     5821 aatgcaagag gccgaagagc tctatgagct tcgtgaagcg cttgaggcat ttgcggtgca
     5881 aaaggcgatt gaaaacctaa ccgacacggg gcttgataag ctgcgctata agatggagtt
     5941 gtacggcagc gacgtgcaga atcggttcac cagagaaagg ctgatgtacg atcaagacat
     6001 tcacctggag attgctcgtt tagccggcaa tgagaccttg gaaaaatccc tgggccatgt
     6061 ttttgagcgg atcgtcctga aacgcagaac cgacgggctc tatgacgccg cgcgcggcgt
     6121 taacgcccat caacagcatc tacggctctt gcgagcgatg gagcagcgca acgtcacgga
     6181 ggcggtcagc atcgtgcggg cgcacatccg caaaggaaaa cagaacgtgc tttcagattt
     6241 ggagcaaagg ctggcaatcc gtaaacgcgc aacagcaacg gtcgaagaaa gagaaaaaca
     6301 aacatacaga aaggaagaga aatggccgca gatttagtga taaaaaatgg ttgggtggta
     6361 acacccgagg aaacatttca aggcggggtt gccatcagca acgagaaaat cgttgcgatc
     6421 ggcgcgaacg atgtattgcc tcaggggaaa gaagaaatcg acgccaaggg gaaacacatt
     6481 cttccgggga tcattgatgg acacgttcat ttcagggaac caggcatgac ccacaaggag
     6541 gacttcacca ccgggtcgac ggctgccgtt tgtggcggaa tcacctcggt cgttgacatg
     6601 ccgaacaccg ttccccctac ggctcaagcc gagcaggtga aaataaaaca acagctgggc
     6661 gaggccaagt cactggtcga cttcggcgtc accggcgtgg ttgtgcaaac caacaccaaa
     6721 gacattctgc cgatggccga cgccggcgcc attggcttca agattttctt cggtgagacc
     6781 atcggcaatc tccctttccc ggacgatggc atgtgcatgg aggccttcga ttacatcgcg
     6841 caatcgaagt tgccgctggg aattcacgcg gagaaccgcc agatcatggc gtactgcacc
     6901 aataagctaa aaagcgacgg acataacgag gcccggtact gggaggcttc tcgcccggat
     6961 atttgcgagg cagaatccgt tcatcacgcg atcttcttcg cggagacgtt cggcaccaag
     7021 ttgcatgtct ttcatatgag ctccaaacag gcagcctaca tggtgcgtga tgccaaagcc
     7081 cgaggtatga ggatcaccgc cgaaaccggc ccccattatc tcctgcgcga acccaacgat
     7141 atggatcaag tcggctcatt gctgaaaatg aacccgccgg tgcgaactaa ggatcacggc
     7201 gaggttctgt ggcagggtct tcgtgaagga ttcgtcgaca tgctagccac ggaccattca
     7261 ccgcacacgc ttgaggaaaa aggttcggac atttatggaa agctcaccaa accggcgatc
     7321 tgggactgca tctcgggttt ctgcggggtt gaaaccggtg ttcctttgat cttgactgaa
     7381 gtgaacaagg gtcgcttgac gctgaaccag tatgtcaaag tagcgtgcga aaatccggct
     7441 aaggtttggc aggtctatcc aaaaaagggc gccattaggc taggctcgga cggcgatatc
     7501 accattgtcg atatggacaa ggaaggcacc atcgacgtca ataaactgca cagcaagaac
     7561 aagccctctc catggcacgg ttggaaagtc aaaggtatgc ccgtatgcac agttgtgcga
     7621 ggtcatgtgc aaatgcgcga cggtgaacct tgtggtaaac cgatcgggcg catgctcagg
     7681 cctttgatct agttttcaaa gtcgattctc agcaaaggtc cagcggcata agccgaatgg
     7741 gttccagtta gttgttgacg tgttcttttc gtcctggatc gcaaaattgg tttggccgct
     7801 ggccgcgcgc gttcgcaagg aagtagccga tgaaacgcac cgaagctttc gaaatgtacc
     7861 aaccgagtac ccttagagag gcgagcctgc tcctgaaaga gaaaggccct ggtggccgtt
     7921 ttctcgcagg aggcaccgac ctcgtgatcg cgatgaagga gaaggggttg gtgccgaaat
     7981 atgttgtcga tctcaaaagg cttccaggct tatccggcat ccgtgaaaat agcgacggca
     8041 gcattaccat tggtgcgttg acgaccatgc gtgagatcga gatatctccg ctgctcacca
     8101 agaagtatcc ttttctttgc caaagcgcgg cggaagtggg ttcaattcag attcgcaacc
     8161 gggcaaccat cggcggcaac atgtcgaacg ccacgccttc cgcagatgtc gcgccaagcc
     8221 tgattgcact tcaagctaca gcaaagattg cctgcgcccg cggcgaacgc agcgtagcga
     8281 tcgaagaatt cttccgcggt cccgggctga ataccatgga ttcggacgag attcttactg
     8341 aaatcacgat cccgaaaacg agcccgcggc tcgtcggcga ctacatcaag ttctcgcccc
     8401 gcgagatgat ggatttggca tacgtcggta tcgccgtagc gtacatactc ggcgaaaaag
     8461 aaaatagatg ctcgcaagtg cggattgttc tcggcgccgt cgctccgacc ccgattcgcg
     8521 cgaaaaaggc ggaagccgca ctagaaggcc aagttctctc cgaagacttg gctgagaagg
     8581 ttggagcgat cgccgcggaa gaggcaagac cgatcagcga cgttcgctca tcagcggatt
     8641 atcggcgcgc catggtcgga gctatgacca aacgggcgct gctcaatgca acggtcggtc
     8701 cggcgaggtc ctgggtcgag cggcgcgacc gtagatatta aaacggcaga ttggagtgat
     8761 ggagtaacgg aatgttgggc cggacttgtg aaatcggtac tccaacactc cattatttca
     8821 atttgggttc cagagggcag tcatgaatac gaaaatccaa tttacgttga atgggaaaag
     8881 catcacaagg gaagttgcca gtcatcgtct gttgctcgac ttgctccgcg atgaaatcgg
     8941 gcttaccgga accaaggaag gctgcggcac cggcgactg
//
LOCUS       DASUVS010000337        11960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_578562, whole genome shotgun sequence.
ACCESSION   DASUVS010000337 DASUVS010000000
VERSION     DASUVS010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11960
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578562"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            581..1219
                     /locus_tag="VF182_18050"
     CDS             581..1219
                     /locus_tag="VF182_18050"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEX6439041.1"
                     /translation="MDLNRIEIDKTGLLFFDILNGYYHQASAAAKARKKPMIDNAVLL
                     MKAARKAHIPILFAKGHHRADEGTTVLINTDTNIPLKPWPDGIVAMVKPNTVEGQSSA
                     EVIPELEPSPDDYHLPKHRWSAFFQTYLDLALRSKKIDTIIISGGSTDVGVASTVFAG
                     RDMDYNFIVVRDACATNHDQRAHDVLMDLIFPRMSRVRTTQQVLQMIEDAGR"
     gene            1216..2304
                     /locus_tag="VF182_18055"
     CDS             1216..2304
                     /locus_tag="VF182_18055"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6439042.1"
                     /translation="MSCADRSRPLRFSLVKVFAFVVFLACWSANAAYGDSEALLADLN
                     RKSPEERLKILTEGARKERVLSFYGSAPVSNSQDVIRAFNKLYPFIEVRYTRLGAPAL
                     VSRLSTEYQAGLHNADLISIRGTLFPELIEKKVVAKYTSPMAAVMRPGFVDKEGYVSS
                     LYSTGYAFIYNTRNVTAAEVPRSFEDLLHPRWKGRLVMDREEYDIYAGMIGVMGEAKA
                     NVLMKRLVEEQRLTFKRGHTLISQLVAAGEHDLFVDGYVQNAVQLKAANAPVELSFTN
                     PTLVKPPSALAIAAKAPHPYAAALMTDFYLSREAQEILAQKLGYWTVHKDVRWLRESP
                     HGLHIVPQLEWGRKYNQLVQDFRKIAGT"
     gene            complement(2476..3447)
                     /locus_tag="VF182_18060"
     CDS             complement(2476..3447)
                     /locus_tag="VF182_18060"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439043.1"
                     /translation="MFAVFFTCFGSAVFLSESRAQPVKLNVSYTGVGPVNLPVVLAKE
                     AGIFARNGLDVTVIRAQAAVSTMALISGEVGLIQAAAPSVLQSNLGGSGAVYVAGGYT
                     GLDYWLVGHPSIKSAEQLKGAIIGAAGLSGGSYTATQLAVRKLGLNPAKDVSIVAVGG
                     TPERLAALRTGRVQATSLNPPTIFAAEKEGFRILADVSALPFQNVAPVTTRRFIKEHP
                     DIVRRYVKSQVEAVHMMKRDRKTGMKVFMQLMGGFKDTAVLEKSYDVSITDDKLPRNQ
                     YPSLEGIKAVLESLGDRAKEARPTDFVELRFIKELEESGFIQALYKQ"
     gene            4097..5026
                     /locus_tag="VF182_18065"
     CDS             4097..5026
                     /locus_tag="VF182_18065"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6439044.1"
                     /translation="MGFNAGGSFDVWARIIAKHLEKHVPGNPSFIVQNMAGGGSIVAA
                     NYIYRVAQPDGLTLGMVSPGIYLQELAGQKEVQFEWFKYSWIGSAEESARIFYIRSDA
                     PAKALEDLRSVATPVKCGSTGLGTGDYYFPRFLQEAFGLKLAMVPGYQSSPEANLAIE
                     RNEIQCRAGSVQAFFGTEPSKTWAKSGFVRPLAQSGRKRYPNLPDVPTIWELMDKHKS
                     NDADRQLAKILVLPDEIGRPFFGPPAMPADRLKILRDAFGKMMSDPDLIAETKKAGLD
                     PSLTTGESITALGKELGTVSPDLVRRMKQVLEN"
     gene            complement(5304..5630)
                     /locus_tag="VF182_18070"
     CDS             complement(5304..5630)
                     /locus_tag="VF182_18070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose acetyltransferase domain-containing
                     protein"
                     /protein_id="HEX6439045.1"
                     /translation="MSARRLFGRYNASDPADIAGKATLLRELLGEAGHGAAIEPPFYC
                     DYGTQITLGARAFVNFGAVFLDPAQITIGQQAQLGPYVQLLTADHPKDAATRSSGPES
                     AHPINI"
     gene            complement(5627..5728)
                     /locus_tag="VF182_18075"
     CDS             complement(5627..5728)
                     /locus_tag="VF182_18075"
                     /inference="COORDINATES: protein motif:HMM:NF023880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose acetyltransferase domain-containing
                     protein"
                     /protein_id="HEX6439046.1"
                     /translation="MDKPFLPVETERSKMLRGELYSAMDPELVTARG"
     gene            complement(5759..6397)
                     /locus_tag="VF182_18080"
     CDS             complement(5759..6397)
                     /locus_tag="VF182_18080"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6439047.1"
                     /translation="MSVKEVPSDHDFFDPAYVKHWTESISRYRPERTRLFEVFAAEAA
                     RIKKGTLSVFELGCGPGFLAEALLKSCQIARYTLVDFSPEMLELSRSRLKKFQDRTVF
                     IQADFKKADWTAGICAGFDLIVSLQAVHELRHASRIPKLYAQLHSLLLPGGTILICDH
                     VNSSSPSAHRAAHFMTVEEHLSTFQKVGFISAREICPAADLSLMAAEKKPAQ"
     gene            6656..7366
                     /locus_tag="VF182_18085"
     CDS             6656..7366
                     /locus_tag="VF182_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6439048.1"
                     /translation="MILLAGAAFVGSLLAAVAGFGGAAILLPLLVAAFGVREAIPILT
                     VAQLLGNLSRAWFNRSELDLPVVGWFALTGVPAALLGGFLFASAPLPFLTRLLGIFLI
                     GVVVYRRARTGAATRLPLRAFAIVGAVFSFLSALLGSVGPIMIPFFLAYGLVKGALIG
                     TEALATVVMHVTKLVAYGSTSILTNHSVAVGLGLGLIMIFGSLIGKKLLDRLPEKIFI
                     MLIEGTLLIAGLRFLLYG"
     gene            7598..8602
                     /locus_tag="VF182_18090"
     CDS             7598..8602
                     /locus_tag="VF182_18090"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439049.1"
                     /translation="MRFVFRPMTILTAVVLLGTVSSDLFSQTKALKELNISYPLGGSS
                     SFFWVAYRSGSFERQGLRLKPIFIPGGVTALQSLLAKEVSIQLTAGPAAIRAWARGAR
                     EVTFIGAVGNRLDYIIVAHPSVRNANDLKGKRIGVSQLGASPDFIARMGLRRLGLNPE
                     KDVTIVPIGSPGERWSALTAGHVQASLFQAPFTLRARKAGYVSLLDFATQDFEYILSG
                     VLTTRSFINSDRDTVMNFMRGLADGMDFYRDERNREKTMSYLREYYRSNANEELEETI
                     NMYSRVTPGLPAVTAKSIENVIISDSDLSGMGLKAADMLDLSFLQKLAEERKAKGKES
                     "
     gene            complement(8668..9579)
                     /locus_tag="VF182_18095"
     CDS             complement(8668..9579)
                     /locus_tag="VF182_18095"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEX6439050.1"
                     /translation="MSWKSPLGRSHPLTGRIWDVSSAQFIDRETLVKRLAPADFVLLG
                     ERHDNPDHHMLQAEVLRSLLAVGRRPAVGFEMLGLDDASIIAGHLAAAPDDAAGLGRA
                     VNWNQRGWPDWAMYQPIAEAALQAKLRIVATNLPLATARKMTRDGLTALEPRMIRELG
                     LDQPPPEPVFARMAADIRDAHCGYAPEQSLKAMVDVQRARDAQMAQSLIAAGDPGGAI
                     LVAGAGHVRTDYGIPVYLRQKTPDKRVISIAFVEVDEQRTEAQSYALPYPDRRLPFDY
                     VWFTPRVDDEDPCEKFKSQFERMKKAQ"
     gene            complement(9832..11016)
                     /locus_tag="VF182_18100"
     CDS             complement(9832..11016)
                     /locus_tag="VF182_18100"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HEX6439051.1"
                     /translation="MSINEAKPTNQPAAIAAARRNGRPLRIGGGPPNYGDNAAKALSE
                     NWQGVSTDGTPLQGLFPIQQTGVSTQPIREAAEAFLASLDAEQRRHTLFSIDAPEWRL
                     WSNIHRTIMRHGMPLFAMTDRQLDHALALLEESLSAQGFQTARDIMRLNETVMEMTGK
                     LDEFGEDLYWLSIMGTPSEEEPWGWQIDGHHLNVNFFVLGDQVTMTPAFLGSEPVHAL
                     SGKYAGTRVFQAEEARGLALARSLTGEQRGQAVIGASLPKDLFTTAFRDNLELRYQGI
                     RYDQLNGAQHDLLAQLVEIHVGKLRPGHSAIKLAETHSHFKDTYFAWIGGVEEDSVFY
                     YRIHSPVLLVEFDHQAGQAFPQYKEHYKDHIHVIVRTPNGNDYGRDLLRQHYRRSGHH
                     RV"
     gene            11183..>11960
                     /locus_tag="VF182_18105"
     CDS             11183..>11960
                     /locus_tag="VF182_18105"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439052.1"
                     /translation="MQLIMKSLRIIFIGLAYLLISTSNLWSAQVVRVGYPSLATGFAA
                     SWVTADKDIWKKHGLDVELIFLRGGSRTVSALISGSVDFILGSDLGITTAIIQGASLT
                     RIGVTTNTLGYSMVTQPGIKAVRDLKGKVIGITPGRDAAYARVVKLLRDNGMDSAKDV
                     TFLSVGDGGPAARVAALSSGVIHATMFTPPSDKISEKAGMSILTRLDVANVGGGINTT
                     TGFIQKNRPQALAFLRGYMEGIHYLKANKDESLKIFSKYVR"
BASE COUNT         2473 a         3447 c         3455 g         2585 t
ORIGIN      
        1 cttcggcctt ggcgcccgcg taaaggatct gttcgcggtc tgcgcccata tagctcacca
       61 actcgctcag ggttgccggc ttgcgcgtct gggccgaggc cggagatggg aaaacgataa
      121 gggcgaggat cagtgcgacc actttcattt tcaaagccct cccggatttg tggtttttcg
      181 cggtgcttgc cctatatcgc ccgcggaggt ttcagtcaac gtagtcccgc aaatttccag
      241 gtcggcacgg tcaaggacgc accaaggggt tctctgccgc catcaggctc aagtctgcgg
      301 cgggacagat ttctcttgcg ctatgaaacc aactttctag aaggtcgaca ggtgctcttc
      361 gaccgtcatg cagtgggaga ggtgtttagc ttacggtctt gtaagcgata gacgcgaagc
      421 aaatcggcct tcgttctatt tcaatcgcca attctttgct tctcgtcgat gagcttatga
      481 acccgccgag agcgtgactg attcgaacga cttctgattt ttgaagtcta gggcttccat
      541 ggaatttgta tcgactcaat ccataagcta aaagcggtgc atggacttga atcgaatcga
      601 gattgataag acaggcttgc tcttcttcga tattttgaac ggctactatc atcaggcgag
      661 cgcggctgcg aaggcgcgca agaagccgat gatcgacaac gcggtgctct tgatgaaagc
      721 ggcgcggaag gcgcacattc cgattctctt tgccaagggg catcatcggg cggacgaggg
      781 cacgacggtt ttgatcaata ccgataccaa tattccgttg aagccgtggc ccgatggtat
      841 cgtcgccatg gtcaaaccga acaccgtgga ggggcagagc agcgcggaag taattccgga
      901 gctcgagcct agtcccgatg actatcatct gccgaagcat cgctggagcg cattttttca
      961 gacctatctc gacctcgccc tgcgatcgaa aaaaatcgac acgatcatta tctcgggcgg
     1021 ttcgaccgac gttggtgtcg catccacggt attcgccggg cgcgacatgg actacaactt
     1081 catcgtggtc cgcgatgcgt gcgccaccaa ccacgatcaa cgggcgcacg acgttctgat
     1141 ggatctcatt tttccgcgca tgtcgcgggt gagaaccacg cagcaggtct tgcagatgat
     1201 cgaggatgcc ggccgatgag ctgcgccgac agaagccgcc cgttgcgctt ttctttggtg
     1261 aaagtgttcg cgttcgttgt ctttctcgcc tgctggagtg ccaacgccgc ctatggagat
     1321 agcgaggctc tactcgccga tttgaaccgc aagtcgccgg aggaaaggct aaagattctg
     1381 acggagggcg cgaggaagga gcgcgtgctg tccttttacg gttcggctcc ggtcagcaac
     1441 tcccaggacg tcatcagggc tttcaacaaa ctctatccgt tcatcgaagt ccgctatacc
     1501 cggctcggcg cgcccgccct ggtgagccgg ttgagcacgg agtatcaagc cggactgcac
     1561 aacgccgatc tgatctcgat ccgagggact ttgtttcccg agctgatcga gaaaaaagtc
     1621 gtcgcgaaat acaccagccc gatggcggct gttatgcgtc cgggcttcgt cgacaaagaa
     1681 ggatacgtct cgagcctcta ttcgaccggc tatgccttta tctacaacac ccggaacgtc
     1741 acagcggcgg aagtgccgcg gtcgttcgaa gacctgctcc atccgagatg gaaggggcgg
     1801 ctggtcatgg atcgggaaga gtacgatatc tatgccggca tgatcggcgt catgggcgag
     1861 gccaaggcga atgttctaat gaaaaggctc gtcgaagagc agcgccttac gtttaagcgc
     1921 ggccacacgc tgatcagcca gctcgtcgct gccggcgagc acgatctttt cgtcgacggc
     1981 tacgtgcaga acgcggtgca gcttaaagcc gccaatgctc cggtcgagct gagcttcacc
     2041 aatccgacgc tcgtaaaacc gccttcggca ttggcgatcg cggccaaagc gcctcacccg
     2101 tatgccgccg cgttgatgac ggatttctat ctttctcgag aggcccagga aatccttgcg
     2161 cagaagctgg gctactggac ggtgcacaaa gacgttcgct ggctgcgaga gtcccctcac
     2221 ggccttcaca tcgtgccgca attggaatgg ggccgaaaat acaaccagct ggtccaggat
     2281 ttcagaaaga ttgctgggac gtgaccgagg tgttcggcac gacgaaatag cgcaagctcg
     2341 gggcaagcat cagtagcaca gtagggtcat agggtcaggt cctgcgttac gacaattgaa
     2401 ggtaagttgg ggtcgcccat gacagcccgt gacagcaaaa ggagcccggt acgattggaa
     2461 tgcccgattg agctctcact gcttataaag cgcttggatg aatccgctct cttcgagttc
     2521 cttgatgaag cgcaactcga cgaagtccgt ggggcgcgcc tctttggctc tgtcgcccag
     2581 cgactccaaa accgctttaa tcccttctaa agaaggatat tgattgcgcg gcagcttgtc
     2641 gtcggtaatt gacacatcat agcttttctc cagcacggcg gtgtccttga acccgcccat
     2701 gagctgcatg aagactttca tgccggtttt tctgtcccgc ttcatcatgt gcaccgcttc
     2761 cacttgcgac ttcacatagc ggcgcacgat gtccggatgc tccttgatga agcgccgggt
     2821 cgtaaccggc gcgacgtttt gaaacggcaa cgcgctcacg tccgccaaga tcctgaagcc
     2881 ctccttctca gcggcaaaga tcgtcggcgg gttgagcgag gtcgcctgca cccgtccggt
     2941 gcgcagcgcc gccaggcgct ccggcgttcc acccaccgcg acaatggaaa catctttggc
     3001 aggattgagc ccgagctttc tcaccgccaa ttgcgtcgcc gtgtacgagc cgccggaaag
     3061 acccgcggcg ccgataatcg cgcctttgag ctgttccgcc gacttgatgc tcggatgtcc
     3121 caccagccag taatcgagcc cggtgtagcc gcccgccaca tagaccgctc ccgagccgcc
     3181 gagattgctt tgcaaaaccg acggcgccgc cgcttgaatc aatcccactt cgcctgaaat
     3241 gagagccatc gtcgacaccg cggcctgagc gcgaatcacg gtgacgtcga gaccgttcct
     3301 ggcaaagatc cccgcctctt tcgctaaaac caccggcaga ttgaccggcc ccacaccggt
     3361 atagctgacg ttaagcttca ccggctgcgc ccggctctca gaaagaaaaa cggcagaacc
     3421 aaagcacgtg aaaaacactg cgaacatcac gcgcgtccat ttcaacgcgc tgcttaaagc
     3481 agaactctcc attcgtcgcg accttcgctt ccttgcattc aattgtgatt gccgatcatg
     3541 acagggaaca gttgttccag ggttattagc agcaccgcca gctaactgga aatgaccacc
     3601 ggcgtcaata gccgggtgcc catcggcgcg cggccgctgg cgcaacgcgt tgaagaaagc
     3661 agaaatggcg attttggaca gcgcatggtc gataaccgta cgtcgaagaa cgtagctggc
     3721 aggagcaccg gaaaactaga gatcaggtct tgcgttacga catttcatta tcgcgcagga
     3781 cacgggcccc cttcggaaac ttttagaagc tcgatcactt cgctggtaaa ctttttgtac
     3841 tcgcgatgaa actcggcatc tttgaaagcc ttacggcata gcatcctggg gctgcgaccc
     3901 tcaagttggc cggaccgcaa agcgctgcta tagtcgccga gtgcgccgag aatttctccg
     3961 gcgcgatcaa agaaagaatc ttcagggagt gtgcatcttg agaaacatca tcggcgttat
     4021 ttttgtctgt ttgacttggg tctccgatct gtcggcgcag gcgccgtttt atcagggcaa
     4081 gcaggtgaat attcgggtgg gattcaacgc ggggggatcg ttcgacgttt gggcgcggat
     4141 catcgccaag catttggaga agcatgtgcc gggcaatccg agcttcattg tccagaacat
     4201 ggcgggcggc ggatccatcg tcgcggccaa ctacatctat cgcgttgccc agccggacgg
     4261 actcacgctc ggcatggtca gcccgggaat ttatttgcag gagctggccg gacaaaagga
     4321 agtgcagttc gaatggttca agtacagttg gattggttcg gcggaagaga gcgcgcggat
     4381 cttctatatt cggtccgacg ctccggccaa ggcgctggag gatctgcgca gcgttgcgac
     4441 gccggtcaag tgcggctcca cgggtctggg caccggcgat tattactttc cgcgatttct
     4501 ccaggaagcc ttcggactca aactcgccat ggtgccgggc tatcagagct cgcccgaagc
     4561 caatctggcg atcgagcgca acgagatcca gtgccgcgcc ggcagtgtgc aagcgttttt
     4621 cggtaccgag ccttcgaaaa cttgggcgaa gagcggtttt gtccgcccgc tcgcgcaaag
     4681 cggacgcaag cgctacccca atcttcccga tgtgccaacg atctgggagt tgatggacaa
     4741 gcacaagtcc aatgacgccg atcgccagct cgcaaaaatc ctcgtgctgc ccgatgaaat
     4801 cggccgcccg tttttcggcc cgcccgccat gcccgccgac cggctgaaaa tattgcgcga
     4861 cgccttcggc aaaatgatga gcgatcccga cctgatcgcc gaaaccaaaa aagccggcct
     4921 cgacccgagc ctgacgaccg gcgaatcgat caccgcgctg ggaaaagaac tgggcaccgt
     4981 ctcgccggac ctggtccgtc gcatgaaaca agtgctggag aactgagacg ttcaggcgga
     5041 accggctagc ctgcaatcgt gaaccgatac gcacgcacac aggatctttg ccctcgcctc
     5101 tcagcgacgg gtgacacgct cacggttcac gaagacgtcg ggactgcaaa tcacacactc
     5161 cggagcacgc gacatgggtt tcccgcagca acgactccgg cgggcagatc gcgaacgacg
     5221 acgctgccgg ctccaacggt tgtgccggcg ccgatcgtca cgccaggaca gacaatcaca
     5281 ccaccgccga gccagacccg ctctcagata ttgatcggat gcgccgattc cggcccggag
     5341 gagcgcgtgg ccgcgtcctt tggatgatct gcggtcaaaa gttgcacgta gggcccaagc
     5401 tgagcttgct ggccgatcgt aatctgcgcc ggatccaaga acaccgcgcc aaaatttaca
     5461 aacgctctgg caccgagcgt gatctgcgta ccgtaatcac aataaaaagg cggctcgatt
     5521 gccgcgccat ggcccgcttc cccgagcagc tcgcgcagca acgttgcttt gccggcgatg
     5581 tcggcgggat cgctcgcgtt gtaacgccca aataggcgcc gcgcactcac ccgcgcgccg
     5641 tgacgagctc cgggtccatt gccgaataca actcaccacg caacatcttg ctgcgctcgg
     5701 tttcgaccgg aagaaaaggc ttatccatgt acgaaagtga gtcttggcat gaccacgctc
     5761 actgcgccgg ctttttctcc gccgccatca ggctcaagtc tgcggctggg cagatttctc
     5821 tggcgctgat gaaaccaact ttctggaacg tcgacaggtg ctcttcgacc gtcatgaagt
     5881 gagcggctcg atgagcggag ggcgacgagg aattgacgtg atcgcaaatc aaaatggttc
     5941 ctccgggaag tagcaggctg tggagctgag cataaagctt ggggatgcgg cttgcgtggc
     6001 gcagctcgtg cacggcctgg agcgagacga tgagatcgaa gccggcgcaa atccccgctg
     6061 tccaatcggc ttttttgaaa tccgcttgaa tgaaaaccgt tcggtcttga aactttttca
     6121 aacgactgcg gctaagctcc agcatctcag gcgagaagtc gacgagcgtg tagcgcgcga
     6181 tctggcaact tttcagcaac gcttcggcga gaaatcccgg tccgcagccg agctcgaaga
     6241 ccgataatgt gccttttttg atccgcgcgg cttcggcggc aaatacctcg aacaatcgcg
     6301 tccgctccgg ccgatatctg ctgatgctct cggtccagtg cttgacgtag gccggatcga
     6361 agaaatcgtg atcgctcggc acttctttca cactcataga cgtcacctta tttctcgtcc
     6421 gccgcggatg caacgaattt gctgggtccg gcctgcgtac atgaacgggc cgagtgttag
     6481 gacccggagt ttgatccgtt tagaatcgat ttaccccaga tactctcttc tcactctctc
     6541 tgcggttaaa accgattgcc gcttatcgta aggtgaaaat ttactcctat gcttcggttg
     6601 gagccgattc cagttggcgg ctcgtggtat ggatatattc atgtctgtcg cgaatatgat
     6661 cctgctggcg ggcgccgctt tcgtgggctc tttgttggcg gcggtggccg gtttcggcgg
     6721 cgcggccatc ctattgccgc tgctcgtggc cgcgttcggc gtgcgtgagg cgatccccat
     6781 tctgaccgtg gcgcagttgt tggggaactt gagccgcgca tggttcaacc gaagtgagct
     6841 ggatctcccc gtcgtcggtt ggttcgctct gaccggtgtg cccgccgcac ttctcggcgg
     6901 tttcctcttc gcttcggcgc ccctgccgtt tcttacccgt ctcctcggca tttttctgat
     6961 tggcgtggtg gtctaccgcc gggcccgcac aggcgccgcg acgcgactcc cgctccgcgc
     7021 gtttgccatc gtcggcgccg tgttcagctt tctctccgcg ctattgggaa gcgtcggccc
     7081 gatcatgatt cctttcttcc tggcctacgg gctggtcaaa ggcgccctca tcggcaccga
     7141 agcgctcgcg accgtcgtga tgcacgtcac caaactggtc gcctacggca gcacctcgat
     7201 cctgacgaac catagcgtcg ctgtggggct gggcctcgga ttgatcatga ttttcggttc
     7261 tttgatcgga aaaaaacttt tggatcggct ccccgaaaag atatttatca tgctcattga
     7321 gggaacgctt ttaatcgctg gattgagatt cctgctctat ggctgagatc aatcattcat
     7381 cggcaaagct tcgcagagcg atccatttaa atccaccatc gtgttcagaa ttttgtatcc
     7441 cgactatcct gtgagtcatt gcagcattcc cgcggtctga tcgctttcca tctcagtctg
     7501 agccgcggcg tcgcgtaagc gaaccactaa actcctggac tttaaccggc tctctgaggg
     7561 agagtggcaa caataaagtc acgaggagga taaatcgatg agattcgtct ttcgcccaat
     7621 gacgatcctg acggcggtgg tgcttttggg aacagtgtcc agtgatctgt tttcccagac
     7681 gaaggcattg aaggaactca atatctcgta ccctctcggc ggctcgagca gttttttttg
     7741 ggttgcctat cgttcaggtt ccttcgaaag acagggtttg agacttaaac cgatcttcat
     7801 cccgggaggt gtcaccgcgc tccagtcgct gctggcgaag gaagtatcga ttcagttgac
     7861 cgccggtccg gccgccattc gcgcctgggc tcgaggcgcg cgagaggtca cgtttattgg
     7921 agcagtcggt aaccggctgg attacattat cgtcgcccat ccctcggtgc gtaatgccaa
     7981 cgatttgaaa ggtaaacgaa tcggcgtgag tcaactcggc gccagccccg atttcatcgc
     8041 ccggatggga ctgcggcgct tggggctgaa tccggaaaag gacgttacca tcgtgccgat
     8101 tggctcgccc ggagaacgct ggtcggcatt gacagccggt catgtccagg cctcgctctt
     8161 ccaggcgccg ttcaccctga gggcgcgcaa agccgggtat gtgtccctgc tcgattttgc
     8221 cacgcaagat tttgagtaca tcctctccgg cgttctgacc acgcgctcct tcattaactc
     8281 ggacagagac acggtaatga atttcatgcg tgggttggcg gacgggatgg acttttatcg
     8341 cgacgagaga aatcgcgaga agacgatgag ctatcttcgt gaatactatc gatccaacgc
     8401 caacgaagag ctggaagaga cgatcaacat gtacagccgg gtcactcccg ggcttccggc
     8461 ggtgacagcg aagtccatcg aaaacgttat catcagcgac agcgatcttt ccggcatggg
     8521 cctcaaagcg gccgatatgc tcgatctgtc attcttgcaa aagcttgcgg aagaaagaaa
     8581 agccaaaggt aaagagtcct aaagtagcga ggccgctgga ggcgtccgtt cgagaagaga
     8641 catcggtggc ctcggcccgt gagccgctca ctgggctttc ttcatccgct cgaactggga
     8701 cttgaacttt tcacaagggt cttcgtcgtc gacccgcggc gtgaaccaaa cgtaatcgaa
     8761 aggcaatcgg cgatcggggt aaggcagtgc gtaactctgc gcttcggtcc tctgttcatc
     8821 gacttcgacg aaagcaatgc tgattacccg cttgtccggc gtcttttgcc ttaaataaac
     8881 cgggatgccg taatcggttc ggacgtgacc ggctccggct accaggatcg caccgccggg
     8941 atcacctgcc gcaatcaagc tctgcgccat ctgagcgtcc ctggcccgtt gtacatccac
     9001 catcgctttc aggctttgct ccggtgcgta accgcaatga gcgtcgcgta tatcagccgc
     9061 catcctagcg aacacaggtt cgggcggcgg ctggtcgagc cccaactcgc gtatcatccg
     9121 cggctcgaga gcggtgaggc catccctggt catctttctc gcagtcgcaa gcggaagatt
     9181 ggtcgcgaca attcttaact ttgcttgaag cgccgcctcg gcgatcggct gatacatcgc
     9241 ccaatccggc catccacgtt ggttccaatt cactgccctg ccgagccccg cagcatcatc
     9301 cggagcggcg gcgagatgtc cggcgataat gctggcgtcg tcgaggccga gcatttcaaa
     9361 acccactgcg ggacgtcgtc caacggcgag caggctccgg aggacttcgg cctgcagcat
     9421 gtgatggtcc gggttgtcat ggcgctcgcc gagaaggacg aaatcagcgg gagccaaacg
     9481 cttgaccaga gtctcgcgat cgatgaattg ggcggaggag acgtcccaaa tacgtccggt
     9541 gagaggatga cttcgaccta agggtgattt ccatgacatg cctgagcacc ctgccaggac
     9601 acacgctacg tagcccgcga gcagtgcaag taaagtcatc gcgcgatgcc ttactttgaa
     9661 acccggcggg cgatatccct cgtgcatatg caagtgaaaa gtcccattcc ttcacgaaat
     9721 aaccgcgacc gcgtcgatct ccagcagcag taaagaattt ccaggatcag gtctatcatc
     9781 gctgcacaaa cacatgcgcc ggttaatcat gctcgcttcc gaatcccgat ctcaaactcg
     9841 atgatgcccc gaccgccggt aatgctggcg cagcagatct ctgccgtaat cgttgccgtt
     9901 gggcgtgcgc acgatgacgt gaatatgatc tttgtaatgc tctttgtatt gggggaaggc
     9961 ctggccggct tgatgatcga actcgaccaa caacaccggg ctatggatgc ggtaatagaa
    10021 aacgctgtct tcttccacgc cgccgatcca ggcaaagtaa gtatctttga agtgcgaatg
    10081 ggtttcggcc aatttgatcg ccgagtggcc gggccgcagt ttgccgacgt gaatttcgac
    10141 cagctgcgcc agcaaatcat gctgggcgcc gtttaactgg tcatagcgga tgccttgata
    10201 cctgagttcc aggttatcgc ggaaagccgt ggtgaatagg tctttgggca ggctcgcgcc
    10261 gatcaccgct tggccgcgct gctcgccggt caggctgcgc gctagcgcga ggccgcgcgc
    10321 ttcttcggcc tgaaagaccc gggtgccggc gtatttgccg ctcaaggcat gcacgggctc
    10381 ggagccgaga aaagccggcg tcatagtcac ctgatcgccg agcacgaaaa aattgacgtt
    10441 caaatgatga ccgtcgatct gccagcccca gggctcttct tcggagggtg tgcccatgat
    10501 gctgagccaa tacagatcct cgccgaactc atcgagcttg ccggtcattt ccatgaccgt
    10561 ctcgttaagc cgcatgatgt cccgcgcggt ttgaaacccc tgggcgctga ggctttcttc
    10621 gagcagagcc agcgcgtgat cgagctggcg gtcggtcatg gcaaacaacg gcataccgtg
    10681 gcgcatgatc gtgcggtgga tattgctcca caagcgccat tcgggcgcgt cgatggagaa
    10741 gagcgtgtgg cggcgctgct cggcatcgag cgaggcgaga aacgcttcag cggcttcgcg
    10801 gatcggctgg gtcgaaactc cggtctgctg aatgggaaat agtccctgaa gcggcgtgcc
    10861 gtcggtgcta acgccttgcc agttttcgct gagcgccttg gcggcattgt cgccatagtt
    10921 tggcggcccg ccgccgatgc gcaaggggcg gccgttgcgc cgcgcggcgg caatggccgc
    10981 aggttgattg gtcggtttag cttcgttgat gctcatcacg gttctcctga tctgatgaca
    11041 gtaaaattac gcgctcgcgt ttaccgatgt caaaccgcgc cggcgcattt tcgcgttgac
    11101 tggttccggc tcaggtgtta gctaagcttg actgcggggc cacggcgatc caaacggctg
    11161 ttggtctcga ggattttcgg ctatgcaatt gatcatgaaa tcactgcgaa tcatttttat
    11221 tgggttggca tacttgctga tttcgacttc gaacctttgg agcgcgcagg tcgtgcgcgt
    11281 gggctatcct tcgctcgcca ccgggtttgc cgcctcatgg gtgaccgcgg acaaggacat
    11341 atggaaaaaa cacggcctcg atgtcgagtt gattttcctg cgcggcggct cgcgcacggt
    11401 ttccgccttg atcagcggca gtgtcgattt cattctcggt tcggatctgg gaatcacgac
    11461 ggcgatcatc cagggggcaa gtctgacccg gatcggggtg acgaccaaca ccttgggcta
    11521 ctccatggtc acgcagccgg ggatcaaagc cgtgcgcgat ctcaagggta aggttatcgg
    11581 gattacaccg gggcgagacg ccgcctatgc gcgcgtggtc aagctgttgc gcgacaacgg
    11641 catggactcg gcgaaggatg taacctttct ttcggtcggc gacggcggcc cggcggcgcg
    11701 cgtagcggcc ctgtcgagtg gcgtgattca cgcgaccatg ttcacgccgc catcggacaa
    11761 gatctccgag aaggcgggca tgagcattct tacccggctc gatgtcgcca acgtcggcgg
    11821 cggcatcaat acgacgacgg gatttattca gaaaaaccgg cctcaagcgc tggcctttct
    11881 gcgcggttat atggaaggga tccactactt gaaggcgaac aaagacgaga gcctgaaaat
    11941 tttttccaag tatgtgcgca
//
LOCUS       DASUVS010000338         2572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1890259, whole genome shotgun sequence.
ACCESSION   DASUVS010000338 DASUVS010000000
VERSION     DASUVS010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1890259"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VF182_18110"
     CDS             complement(<1..397)
                     /locus_tag="VF182_18110"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6439053.1"
                     /translation="MADYQHRTIRVGDIKTHFLEAGSGPDLLLLHGGEYGASAEITWK
                     HSIDALARQFHVVAPDILGWGATEKIYSFSDPAGYRISHMKRFLETIAVDNAYFVGNS
                     AGGGLILRASVLAPAPFQIRKMVTICGNAS"
     gene            complement(410..1180)
                     /locus_tag="VF182_18115"
     CDS             complement(410..1180)
                     /locus_tag="VF182_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6439054.1"
                     /translation="MIEGLKDKVVIVTGGGHGIGRAYCLGFGGAGAKVVVADIDEPAS
                     VKVAKEVNSQTDAQSLSVKVDVANEASTQNMVKLALERFGKVDILINNAAIFATIPMN
                     RGGIDSIDPDEWDRMMSVNLKGLFFCCRAVLPSMRKQKSGKIVNISSGTALNGSAGRI
                     HYVTSKAGVIGFTRTLAREVGEDNINVNALAPGSTLSEENPSEEILKMRGARLTDRCL
                     KRVQLPKDLVGTVLFLSSPLSDFMTGQTVAVDGGVAFL"
     gene            1472..2167
                     /locus_tag="VF182_18120"
     CDS             1472..2167
                     /locus_tag="VF182_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6439055.1"
                     /translation="MIFSLMLFSLFLRLAPAESAVGGEAAAPIYGDPGVSPSIPLKKA
                     DEDFVRDSVAKYGSRRNASKAFATQGWKLVRNNQPELALQDFIRAWQLDGKNYQAFWG
                     FGAILSEQGKIKEAIEQLEIARELNDDSSQRVQLLTDIGILHSEYATRMPPSDQLGRA
                     HRFVLANNRFAESIEIDQNDAASWRAWAISLYAQERYSEAWIKAKRAMDLKAEPFPEG
                     FLKRLREKMPQPD"
     gene            2312..>2572
                     /locus_tag="VF182_18125"
     CDS             2312..>2572
                     /locus_tag="VF182_18125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439056.1"
                     /translation="MYRECTRVLGSALLALTGCLHTDALAQGASQVEQLLTLSNGNFP
                     VVLVAPHGGREAIPGIGIRQGANVLQFKTRRDHHTAELAAAIA"
BASE COUNT          588 a          693 c          732 g          559 t
ORIGIN      
        1 cgctggcgtt gccgcaaatc gtgaccatct tgcggatctg aaatggcgcc ggcgcgagta
       61 cggaggcgcg caggatcaag cctcctccgg cagagttgcc aacaaagtag gcgttatcga
      121 cggcgatcgt ttccagaaac cttttcatat ggctgatgcg gtagccggcc ggatcggaaa
      181 agctgtaaat cttctcggtc gcgccccaac cgagaatgtc cggcgcaacg acgtgaaact
      241 gccgcgccag ggcgtcaatg ctgtgtttcc acgtgatttc ggccgatgcc ccgtactcgc
      301 cgccgtgaag caacagcaga tcaggaccgc tgccggcctc aagaaaatga gtcttgatgt
      361 cgcccacacg gatcgttcga tgctgataat cggccacgga gacggacacc tataagaacg
      421 ccacaccacc atcgacggca acagtttggc cggtcatgaa atcgctcaaa ggagaagata
      481 ggaacaaaac ggttccgacc aagtccttgg gtagttggac tcgttttaaa catcgatcgg
      541 tcaaacgcgc gccgcgcatt ttaagaatct cttcgctggg attttcttcc gaaagcgttg
      601 agccgggcgc gagggcattg acgttgatgt tgtcttcgcc gacttcgcgg gccagggttc
      661 gcgtgaaacc aatcacgccg gcttttgacg tcacatagtg aatgcgcccg gcgctgccgt
      721 tcaaggcggt gcccgacgaa atgttgacga tcttgccgga tttctgcttg cgcatactcg
      781 gcagcaccgc gcggcagcaa aagaagaggc ctttgagatt caccgacatc attcgatccc
      841 attcgtcagg atcgatggaa tcgataccgc cccggttcat gggaatggtt gcgaagatcg
      901 cggcgttgtt gattaagata tcgaccttgc cgaagcgttc gagagcaagc ttgaccatat
      961 tctgggtcga cgcctcattg gcaacgtcga cctttaccga caatgattgc gcatcggttt
     1021 gagaattcac ttctttggcg accttgacgg aggctggttc atcgatatcg gcgaccacga
     1081 ctttcgcgcc ggcgccaccg aaaccgaggc aataagcccg gccaatccca tggccgccgc
     1141 ccgttacgat aacgacttta tctttgagtc cttcgatcat tctcaactcc tttgatttgg
     1201 attccagtca aaatatcctc ggacctgagg agggtcaagc gattttgcgc ctgccgaggc
     1261 tcgtcgtatg gaacaaactc cggagattaa cttggcactc aaaggcaatc aatcttgttg
     1321 agagcgtttg ccatgttatg attgctcgat gctatatcag tgcgaccgca cgaagattgg
     1381 gccgtatcgg ggtcgaaccc aggccggggt agagcttcaa ggtcttgaac aaagatatca
     1441 cgtgaggcca tggcagagcg tcggatcggg gatgattttt tcgcttatgt tgttctcatt
     1501 gtttcttcga cttgcaccag ccgagtccgc agtcggcggt gaggcggccg cgcccattta
     1561 tggggatccc ggagtcagtc catcgatacc gctgaaaaaa gccgacgaag atttcgtccg
     1621 cgactctgtg gcgaaatacg gcagccgccg aaacgccagc aaagcatttg cgacgcaggg
     1681 ttggaagctg gtgaggaaca atcagcccga gttggcgctc caggatttca tacgagcttg
     1741 gcaactcgat ggtaaaaatt atcaggcatt ctgggggttc ggcgcgatcc tgagcgagca
     1801 gggaaagatc aaggaagcca tcgaacagct agagattgcg cgggaactga acgacgattc
     1861 atcgcaacgg gtgcagttgc tcaccgatat cgggatcttg cattcggaat acgcgacgcg
     1921 aatgcccccg tcggaccaac ttggtcgggc acatcgtttc gttcttgcaa ataaccggtt
     1981 cgccgaaagc atcgagatcg atcaaaacga cgcggcgagt tggcgtgcct gggcgatttc
     2041 gctctacgcg caggagcggt actcggaggc ctggatcaaa gcgaagcgcg ctatggatct
     2101 taaggccgaa ccgtttcccg aggggttttt aaagcgtctc cgcgagaaga tgccgcaacc
     2161 tgactagagt tgacggagca ttgaaaactc tcttcacatt cggtcaagac gtctcagtgg
     2221 cgagacctaa tcgccgtgtc atgtccgtgc gcacgtgatc gagaaattcg aacaaacaca
     2281 aacacgtacc acgaacacgg atcgaggtag tatgtaccgc gagtgcacac gagttctggg
     2341 ttcggctctg ctcgcgctca caggttgttt gcacaccgat gctttggccc agggcgcatc
     2401 acaagttgaa caattgctga cgctgtccaa cggtaacttt ccggttgttc tggtcgcgcc
     2461 gcacggcggc cgtgaggcca ttccaggaat cggcattcgc cagggcgcga atgtgctgca
     2521 gttcaaaacc cggcgtgatc atcataccgc ggaattagcc gcagctattg cg
//
LOCUS       DASUVS010000339         4269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1673482, whole genome shotgun sequence.
ACCESSION   DASUVS010000339 DASUVS010000000
VERSION     DASUVS010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673482"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VF182_18130"
     CDS             <1..385
                     /locus_tag="VF182_18130"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX6439057.1"
                     /translation="ASIEELKATLGALRVEEIDAIFEVPDPMVVNEAQLIIDAAMAKK
                     LPTMFFFPSEVVKGGLASYSVDLDAVGRVSAKHVQRILSGSKPSNLPVEGVDKIFLAI
                     NLKTAKQIGLTIPANVLARADKVIR"
     gene            465..1448
                     /locus_tag="VF182_18135"
     CDS             465..1448
                     /locus_tag="VF182_18135"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439058.1"
                     /translation="MKAVSRLWAVVSILAAILCASFSRVEGQITSKLARIGYLTGSGL
                     SISDAFMDELRELGYADGKNITIKFQTAQGDLGNLPRLADKLASSPVDVLFAADFNSA
                     LAAKNATDKIPVVFQTLGDPVASGLVHSLPRPGRNLTGVAGFGPELGGKRLELIKEVL
                     PDISRVAFLTDPSNAAGAVTLRETQIAANSFAVKLQVHKVKNAAELKNAFMLMKKNQV
                     DALLVNHDPTLTAQRNYIVRLVEESRLPAIYVETLWEASGGLIIYGPNLANQFRRAAT
                     YVDKIIKGAKPADLPVEQPTKFDLVINLKAAKQIGLTIPPNVLARAERVIR"
     gene            1546..2067
                     /locus_tag="VF182_18140"
     CDS             1546..2067
                     /locus_tag="VF182_18140"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439059.1"
                     /translation="MKGVAIGCLFATLVLFSFHPAEAQKSPVVRRIGFLSVSGGPSGA
                     KEVFRRSLGGLGYAEGRNIVIEYRGAEGRPRLLELANELVRDKVEVIVASGGPAARSA
                     QDATKTIPIVFATSGEPVESGFVDSIARPGGNMTGMTWLSFELVGKRLELLKEAVPGV
                     SRVAVLSNPQHPR"
     gene            2108..2521
                     /locus_tag="VF182_18145"
     CDS             2108..2521
                     /locus_tag="VF182_18145"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX6439060.1"
                     /translation="MGITLDHYQTRTRAEFDAAYDAISKQNVNGLLVFPEGVTLANRL
                     GIIDFAAKRQLPSMLGWKEFVEAGGLMAYGPNRDESFRRIALYVDKILKGTNPADLPV
                     ERPTKFELVINLKTAKQIGLTIPPNVLARADRVIK"
     gene            2577..3560
                     /locus_tag="VF182_18150"
     CDS             2577..3560
                     /locus_tag="VF182_18150"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439061.1"
                     /translation="MIRIFVGLLLTVLLVTVSVVEAQQPTKVPRIGFLIVDSPSAAST
                     RVEAFKKGLQELGYVEGKNITLEYRYAEGKADRLPQLASELVRLKIDVLVTGGGNQVA
                     MAAMKATGTIPIVMTNVADPVGSGLVASLARPGGIITGLTSVTYDLSGKRLELIKDTL
                     PKLGRIAVLYDPSDRAKVVEFKEMQAAAGSFAVQLQSLEVRRAEDFEGAFKSGNRARA
                     EALIVLPTTITNTHRRPIIQLAEGNRLPAIYPDQEFVDAGGLMSYGPIYADLWRRAAT
                     YVDKILKGAKPGEIPVEQPTKFQLVINLKAAKQIGLTIPPSVVARADRVLR"
     gene            3614..>4269
                     /locus_tag="VF182_18155"
     CDS             3614..>4269
                     /locus_tag="VF182_18155"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439062.1"
                     /translation="MSGFPSESFSDKRKAAIQNPKWLRLWVFTFMLMVSAAVAQAQQV
                     KKVHRIGVLSSGDAPSESARSEGVRLALRELGYIEGQNIAIEYRYAEGKRDWAPKLAA
                     ELARLNVDIIVVSGGTVWVRAAKNATQTIPIVMTGAGLDPVEAGLVESLPHRGGNITG
                     ITTQFTREVGGKRLELLKETVPKVVRVAVLYDPIAPGTAREVKEILPVAAHALGMTIQ
                     P"
BASE COUNT         1037 a         1074 c         1216 g          942 t
ORIGIN      
        1 cgcttccatc gaagaactca aagcgactct gggagcgctc agggtcgagg agattgatgc
       61 catctttgag gtgcccgacc caatggtggt caacgaggcc caactgatca tcgacgccgc
      121 gatggctaag aagttgccga ctatgttctt ttttccgagt gaagtggtca aaggaggtct
      181 cgccagttac agtgttgacc tcgacgcggt tggccgcgtg tcagcgaagc acgtccagcg
      241 cattctcagt ggtagtaagc cctcgaactt gccggtagag ggcgtcgata agattttcct
      301 ggcgatcaat ctcaagaccg cgaagcaaat tggcctcacg attccagcga atgtcttggc
      361 gagagccgat aaagtaatcc gatgagcgag ctgatccaaa gttcaaaatc gagaatcgaa
      421 ttcaatgatc cccgttaagt gctgagagca gaacgggtga ttcgatgaaa gccgtcagtc
      481 gtctgtgggc agtagttagc atcctggctg ctattctctg tgcatcgttc tcccgtgttg
      541 agggccagat aacaagcaaa cttgcacgga tcggttatct gaccggaagc ggtttatcta
      601 tcagtgatgc cttcatggac gaattgcgag agctgggcta cgcggacggc aaaaatatca
      661 ccattaaatt ccagacagcg caaggcgatc ttggcaatct accaagattg gccgataaac
      721 tcgcaagttc acctgtggat gtgctttttg ctgcagattt caattccgct ctcgccgcca
      781 aaaatgccac tgataagata cccgtcgttt ttcaaacact cggagatccg gttgcgagcg
      841 gattggtcca tagcctcccc cgacccggtc ggaatctaac tggagttgca ggttttgggc
      901 ctgagttggg cggaaaacgg ctcgagctca taaaagaagt tcttcccgat atttcccggg
      961 tagcgtttct gacagaccca tccaacgcgg caggtgctgt gactttgcgc gaaacgcaga
     1021 tcgctgcaaa ttctttcgct gtgaagctgc aagtacacaa agtgaagaac gctgccgaac
     1081 taaagaacgc ctttatgttg atgaaaaaga accaggtaga tgctttgctg gtcaatcacg
     1141 atccgactct gacggcgcaa cgaaattaca tcgttcggct cgttgaagag tctcgtctgc
     1201 ctgccattta tgtagaaaca ttgtgggagg caagtggagg attaataatc tatgggccga
     1261 atctggctaa ccaatttcgt cgtgctgcta cctacgtcga caaaatcatc aaaggcgcca
     1321 agccagccga cttgcctgtc gagcaaccga ccaagttcga tctggtcatc aatctgaaag
     1381 cggcgaaaca aatcggattg acgattccac cgaacgtgct ggcgcgggcg gagcgggtga
     1441 tcagatgaca cagatccttc accggagttt atcctgagcc acgtcgaagg ttcaggacaa
     1501 gtttttgctt gccgattctc gattttgact aagagagagg aagccatgaa aggggtggca
     1561 atcggttgtc tcttcgcaac tttggtgctg ttttcatttc acccggctga agcccaaaag
     1621 tcaccagtag ttcgaaggat aggatttctc agtgtgtcgg gaggcccctc gggggccaaa
     1681 gaggtgttcc gacgaagtct cggtgggctt ggctatgcgg aaggtcgtaa cattgtcatt
     1741 gagtaccgcg gcgccgaggg tagaccacgg ctgcttgagc tggctaatga gctagtccga
     1801 gataaggttg aagtgatcgt tgcgagtggc gggccagcag ctcgatctgc tcaagacgca
     1861 accaagacca ttccgattgt tttcgctact agcggcgaac cggttgagag cggttttgtc
     1921 gacagcatcg cgcggccggg aggaaacatg accggcatga cctggctgtc atttgaactt
     1981 gtcggcaaac ggctggagct tctcaaagag gcggtgcctg gggtctcccg tgtggccgtt
     2041 ctatcaaatc cccagcaccc aaggtgaacg ccgggaattg cgggagacgc aaaccagtgc
     2101 acgagccatg ggaataactc tcgaccacta tcaaacgaga acgcgcgccg aattcgacgc
     2161 cgcctacgat gccataagca agcaaaacgt caacggtctt ctagtttttc ccgaaggcgt
     2221 aacattggct aatcgactgg ggatcatcga cttcgccgcc aagcgccaac ttccgagcat
     2281 gctcggatgg aaagagtttg tggaagccgg tggcctcatg gcctatgggc cgaaccgtga
     2341 cgagtcattc aggagaatcg ccctgtacgt ggacaagatc ctaaagggca caaatcccgc
     2401 tgatctcccg gtggagcggc ctaccaagtt tgagctggtg atcaatctga aaactgccaa
     2461 gcagatcggc ttgacgattc cgcccaacgt gctggcgcga gcggatcgag taataaaatg
     2521 acagcgagca gcgagcagaa aacagctagc agcaagaaac ccggatcaaa gcccaagtga
     2581 taaggatttt tgtcggctta ctgcttactg tccttctggt tacggtttca gtggtcgagg
     2641 cgcagcagcc aacgaaggtg ccacgtattg gattcctgat tgttgactct ccttccgctg
     2701 cgtccacccg tgttgaagcg ttcaaaaaag gactgcaaga gctcggatat gtggagggga
     2761 aaaacatcac tctcgagtat cgatatgctg agggcaaagc cgaccggctg ccgcaactcg
     2821 cgagcgaact ggtccgcctc aaaattgatg ttctggttac aggcggtggc aatcaagttg
     2881 ccatggctgc gatgaaggct actggaacga tcccaattgt aatgacgaat gttgccgatc
     2941 cagtcggaag cggtttggtc gcgagtctcg cacggcccgg cgggatcatt acgggattga
     3001 cctcggtgac atacgacttg agcggaaaac gcttggagtt aatcaaagat actcttccca
     3061 agctcggccg tattgccgtt ctgtatgatc caagcgaccg ggctaaagtc gtcgagttca
     3121 aggaaatgca ggccgcggcc ggctcattcg cggtgcaact acaatccctt gaagtgcgaa
     3181 gggcggaaga cttcgagggc gcattcaaat caggaaaccg cgcgcgggcg gaagcgctaa
     3241 tcgtgctgcc gaccaccata accaatactc atcggagacc cattatccaa ctcgcggaag
     3301 gtaaccgtct gccggcgatt tatccggacc aggagttcgt cgacgccggt ggcctgatgt
     3361 cttatggtcc catctacgct gacttgtggc gccgcgccgc cacctatgtg gataaaattc
     3421 tcaaaggtgc gaagccaggt gaaatacctg tggagcagcc gactaagttc cagctggtga
     3481 tcaatcttaa agccgccaag caaatcggcc ttacgattcc gccgagcgtg gtggcgagag
     3541 cagaccgggt gctcagatga aattttggat tttcgatttt agattttgga ttcatatcca
     3601 ggagaactaa ccgatgagtg gatttccgtc ggagtctttt tccgataagc gaaaagcggc
     3661 aatccaaaac ccaaaatggc tgaggctttg ggtatttact ttcatgctca tggtaagtgc
     3721 ggctgtggct caggcgcagc aggtgaagaa agttcaccgc ataggtgttc tgtcgtcggg
     3781 cgatgcacct agcgagtccg cccgttccga gggagttcgg ctggcgctgc gcgagcttgg
     3841 ctacatagaa ggacagaaca tcgccattga gtatcgatat gcggagggaa aacgcgattg
     3901 ggcccccaaa cttgcggccg agttggcacg cctcaacgtc gatatcatag tggtgtcagg
     3961 agggaccgta tgggtccggg cagccaagaa tgcgactcaa acgattccta ttgttatgac
     4021 cggcgccggg ctcgatcctg tcgaggcagg tctggttgaa agccttcccc accgaggcgg
     4081 gaacatcaca gggattacga cacaatttac cagagaagta gggggtaagc ggctagagct
     4141 gctcaaggaa actgttccga aagttgtccg tgttgcggtt ctctatgacc ccattgcgcc
     4201 gggcacagca cgcgaggtga aagagattct tcccgtcgcg gcgcatgcgt tgggaatgac
     4261 tattcaacc
//
LOCUS       DASUVS010000340        16043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_799383, whole genome shotgun sequence.
ACCESSION   DASUVS010000340 DASUVS010000000
VERSION     DASUVS010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 16043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16043
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799383"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /gene="mnhG"
                     /locus_tag="VF182_18160"
     CDS             complement(<1..250)
                     /gene="mnhG"
                     /locus_tag="VF182_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HEX6439063.1"
                     /translation="MSEALSAALMLIGAVFGLLAGAGVVRMPDLFMRMQAATKASTLG
                     IGCIMLAVAVHFGELGIATRAVATVIFLFLTAPVAAHMI"
     gene            complement(247..525)
                     /locus_tag="VF182_18165"
     CDS             complement(247..525)
                     /locus_tag="VF182_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX6439064.1"
                     /translation="MSAPWVFIVSFISLSMMAVAIMLAAFRLIRGPSLPDRVVALDLI
                     SILAAGVTAIYAIASGQAVFLDVATILALVSFLGTVAFARYLEKQVSQ"
     gene            complement(525..992)
                     /locus_tag="VF182_18170"
     CDS             complement(525..992)
                     /locus_tag="VF182_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEX6439065.1"
                     /translation="MFLWNIFLAFVWAAVTGRFNLSNVVVGIVLGYAVLFVVQPLMGP
                     SNYFRRIHQAIAFAFFYVWQLVMANLRVAADVMTPGARARPGVLAIPLEARSDAEITM
                     LANLITLTPGSVSLDVSSDRRFLYLHAMYIDDVEQYREDVKTSFERRVLEVLR"
     gene            complement(994..2505)
                     /locus_tag="VF182_18175"
     CDS             complement(994..2505)
                     /locus_tag="VF182_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007350770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit D"
                     /protein_id="HEX6439066.1"
                     /translation="MNVLLILPLLIPLSAATLSLFAWRSRRTQRVLGVVGTALLLGSV
                     LMLLRSVSRGGIQVTQVGAWPAPFGITIVADLFSAIMLVLAGITGMSVAVYSMVTVDR
                     ARERFGYYPLLHALLMGVCGAFLAGDLFNLYVWFEVMLMASFVLMALGGERAQMEGAI
                     KYVTINLIASALFLAAVGMLYGVVGTLNMADLAQKLRDAPRLDLLTAIAMLFLVAFGI
                     KAAVFPFFFWLPASYHTPPVAVSAIFAGLLTKVGVYALIRVFTLLFVQDLGYTHTIIL
                     VLSGLTMVTGVLGAVAQNEIRRVLSFHIISQIGYMTLGLGLFTTAGIAGSIFYIVHHI
                     IVKTNLFLVSGVVYLLRGTFELKSLGGLYSSNPGLAILFLVPALSLAGLPPLSGFFAK
                     LTIVQAGLQAEQYLLVATALMVGLLTLFSMTKIWAEVFWKQAAAGPNAAEASGALAKR
                     GAAWILLAPIIFLAAITITIGLAVEPVYLLASRAAEQLMDPTEYISRVLERSF"
     gene            complement(2502..2846)
                     /locus_tag="VF182_18180"
     CDS             complement(2502..2846)
                     /locus_tag="VF182_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HEX6439067.1"
                     /translation="METVLAFVIGALYAAGLFMMLRPSAVRLIIGISLLGHAANLLIF
                     TIAGLTRARPPLVTGGLTTASAVADPMPQALILTAIVIGFAVQAFAVVLIQRVVQIVG
                     SDDLDQMKTTDS"
     gene            complement(2849..3262)
                     /locus_tag="VF182_18185"
     CDS             complement(2849..3262)
                     /locus_tag="VF182_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit B"
                     /protein_id="HEX6439068.1"
                     /translation="MTSLILSTAARYLLPLLLLFSLFILIRGHNEPGGGFIGGLIAAA
                     AFSLNAIAFDARSTRRTLRVDPRMLIPAGLSIALVSGIVSLFYGEPFMTGKWFSVFIP
                     GLEKLDIGTPLVFDCGVYLLVLGVVLTLILTLAEE"
     gene            complement(3259..5562)
                     /locus_tag="VF182_18190"
     CDS             complement(3259..5562)
                     /locus_tag="VF182_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative monovalent cation/H+ antiporter subunit
                     A"
                     /protein_id="HEX6439069.1"
                     /translation="MLAAIFSSFAASLLAPRLYRMSGRAAGWFLALLPGGLTLYFGSF
                     IAAVSSGEVFLFSYDWAPSLGIRFSFSLDGLSLLFAILISAIGALIIIYSSGYLAGHS
                     QLGRFFVYILLFMGSMLGIVLADNLMTLFVFWELTSISSYLLIGFDHESEDARAAALQ
                     ALLVTSGGGLALLAGFLLLGHIGATMEISALAESGATIRSDPLYRPIVLLVLAGAFTK
                     SAQVPFHFWLPGAMVAPTPVSAYLHSATMVKAGVYLLARLTPVLGGTELWMLALTTAG
                     ATTMAVGAYLALRQTDLKLILAYLTVSALGILVLFIGLGTSEAIIAAVVFLLGHALYK
                     GALFLVVGIVDHETGTRDVGQLRGLLSAMPLTAALAAMAALSLAALPPAFGFIGKELL
                     LEASLGAETGAALMTMALVAASSVFVAAAAMIAIRPFFGRKSAAATRAHEAPFTLWLA
                     PLVLSVCGILFGAMPNLVEAPLVRAGVQAVMREPVSFHLALWHGLNLPLGLSALSLLC
                     GAALYAGRRRVHDAAARLQITSWWGPQHWYGQLLAGMNALAKRQTQLLQNGYLRFYLL
                     IIVAATVALVAAKLFRAIGSIHLPAELDVRFYEWIIAALIPIGALTAIMSRSRLGAVA
                     ALGVVGYSVGLIFVLFSAPDLAMTQFMVETLTVILFVLVFYHLPRFTAFSPRLILVRD
                     AVTALTFGGLITVIVAVGSGIQLYPKISNYFVENSLALAHGRNVVNTILVDFREFDTF
                     GEITVLAVAGIGVYALLRLRPRKEKTS"
     gene            complement(5704..8535)
                     /locus_tag="VF182_18195"
     CDS             complement(5704..8535)
                     /locus_tag="VF182_18195"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX6439070.1"
                     /translation="MSVSETPTGSAKKAPSQTEPYRQSVDQVLRAYVGDAQRGLTSAE
                     ARTRLAKSGRNELTAEKPVPGWKKFLAQFQDVLVILLLLATLISAGLWLLERDTALPY
                     EALAILAVVLLNAVMGYFQQARAEQAVAALRNMSAAHANVIRDGERQNIPAAELVPGD
                     IILIEEGDTIPADARLIQETALQTAEAALTGESLPLSKDIAPIIEEVGVGDRGNMVFS
                     GTAATYGRGKAVVVATGMQTEMGRIAGMLKEAPEETTPLQRELDRVGKLLGFIVVIIA
                     VVMIATIIIVEDVSSFAAILDVLILGVALAVAAVPEGLPAVVTAVLALGVQRMAKRNA
                     IVRHLSAVETLGSATVIASDKTGTLTKNEMTVRAVIAASGRVNIGGTGYAPEGEVWRE
                     GGGTIGEGPLRIELERALATGDLANNAVLQEHNGRWTVQGDPTEGALLVAARKAGLRN
                     HLLDARFQRVGEVPFSSERKLMSTVHTDAETQDRLRVFTKGAPDVLLARCSEELVGAE
                     TRPLTPARRAEIAQSNEALAGEALRTLGVAFRSIPAAAFDAANVDEDVEQNLVFAGLI
                     GMIDPPREEAKAAVARAKAAGIRPIMITGDHPKTAAVIAAELGIAETGRAVTGGELEK
                     MPEDKLERTVEEVAVYARVNPEHKLRIVDALQRRGATVAMTGDGVNDAPALKSADIGV
                     AMGITGTDVSKEAADMVLADDNFATIVAAVEEGRAIFANIRKFLRYLLSSNIGEVMTM
                     FFGVLLADFIGLTATAGQAVVLPLLATQILWINLVTDGAPALALGVDPADPGVMSQPP
                     RPRNEGVITGRMWGGIFFVGAVMAVGTLCVLDGALPGGFLEGSGTTRYAQTMAFTTLM
                     MFQLFNIFNARSDAQSAFTGLFHNHWLWAAIGLSLALHVAVIYTPFLQQAFSTMDLTA
                     GDWLVCAAVASSVLWLRELSKLFTRMRNG"
     gene            complement(8845..11961)
                     /locus_tag="VF182_18200"
     CDS             complement(8845..11961)
                     /locus_tag="VF182_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HEX6439071.1"
                     /translation="MFDLDGVLTKTASVHAAAWKKLFDEFLRQHAAETREAFAPFDID
                     ADYRRYVDGKPRYDGVASFLNARGIGLPLGSAADGPDTQTVHGLGNRKDRYFSEQIEQ
                     HGVEVYAPAIELVRALRELEIKTAVVSSSKNCAAVLRAADIAQLFDTRVDGNDIVRMK
                     IRGKPAPDAFLEAARRLKVEPARAVVIEDAIAGVAAGRAGTFGCVIGVDLAGQSQELR
                     QAGADIVVTSLAEVQIATEPASAWSLVYDRFDPEQEGIREALCALGNGYFATRGAAAW
                     AVMDDVHYPGTYLACGYNRQRTDIAGRIVENEDLVNFPNWLALEFRIDRGEWFDLRRV
                     NILSYRQELDLRRGVLLRSICFEDRGGRRTTLRERRFVSMADMHTAGLELSLTADNWF
                     GGVTVRSGINGRVVNAGAKLYLKFNNKHLEHLGGALIGEDTLYLLVRTCQSNLHVAES
                     ARTQAFVGGEPREYRRHLIDEPGKVAQELTLHLGRGETLSLEKIAAIFSSRDPAISEP
                     ATAARKAIGRAPRFEALLAQHALAWKHLWRRFDIHLRGAPSGFQLNVSMLLRLSMFHL
                     LEAASPNSIGLDLGVPARGWTGEAYQGHVFWDELFIFPFLNFRVPEITRSLLMYRYRR
                     LDEARAAAANAGFRGAMFPWQSGSDGQEETQELNLNPRSNRWVPDKTYLQRHVGSAVA
                     YNVWQYYQVTGDIEFLQAYGAELMIEIARFWSSVATFNPERGRYEIRGVMGPDEFHDG
                     YPDSSSPGLDNNAYTNIMAVWVLCRAFDVLELLTDIRRNELMARNRLSADELEHWRDV
                     SRKMYVPFHDGIISQFEGYERLKELDWEDYRKRYGNIQRLELILEAENDSANRYKVGK
                     QADVLMLFYLFSAEELRGLFERLAYPFEYDTIPRNVAYYDNRSSHGSTLSRVVHAWVL
                     ARSDRPRAMRYFAEALQSDVNDIQGGTTAEGVHLGAMAGSVDLVQRVSTGIEITGDVL
                     RFNPRLPDELERFDMRIRYRSHSLDLRLTRDTLTVRERERGGAPIRLGFRDEIYEFAG
                     GTRVFKLE"
     gene            complement(12232..14316)
                     /gene="ftsH"
                     /locus_tag="VF182_18205"
     CDS             complement(12232..14316)
                     /gene="ftsH"
                     /locus_tag="VF182_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX6439072.1"
                     /translation="MNSTGKQTGRGDSGAASRLQSSSNKPGDSKSGEQWKMPPGRTWL
                     WFVGILLANYLLMRLLIPSPEGPVTVPYTLFKEEVGKGNVESIYSQGESITGRFKTAV
                     TYPPKEEKSEAPAKETKQPKERGATPRPATKTATNFTTTLPAFVDPGLESFLIDNGVE
                     ISAKPIDESGGPLSTLLFGFGPALLFIGFYVWMFRRAAQQGGGMGGLMGIGKSKARRY
                     DQEKDTKVTFEDVAGIDEAENELVEIVDFLKDPKKYTRLGGAAPKGVLLVGAPGTGKT
                     LLARAVAGEAGVPFFSMSAAEFVEMIVGVGAARVRDLFKQAHEQAPAIVFIDELDAIG
                     RARGQVAIGGSSEQEQTLNQILTEMDGFSSREGIIVLAATNQPDVLDKALLRPGRFDR
                     RVVVNLPDKIGREAILKVHTRTVPLAKDASLEELAAATPGFSGADLRNLVNEAALLAA
                     RREQSDVRQKDFLDALEKIVLGPERPLLLSLADKERIAYHEGGHAILGLVVPGADPVN
                     RVTIVPRGQALGVTYQRPDSDRYNYPEAYLRARIVGMLGGRAAEEIVYGTKTTGAESD
                     IEQATGLARRMVTRWGMSERLGLVQLAPRDNPYLRGPDGYGGARQFSEETAEAIDSEL
                     LKIIGESHEEAKRLLTAHRKQLDALAQALVARETLNEKEILEVTGLPPAPPLETRMLP
                     ADGNGSARHPIS"
     gene            14889..15446
                     /locus_tag="VF182_18210"
     CDS             14889..15446
                     /locus_tag="VF182_18210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX6439073.1"
                     /translation="MSNPVRILGIAGSLRRGSYNRAALHAAVQLVPQNAALEVFELDG
                     IPGFNQDEEQNPPAKVAELKQHVRNADAIVIVTPEYNYSVPGVLKNAIDWASRPYGDS
                     AWNGKPVAIMGASIGTFGTARAQYHLRQSFVFLNMFPINQPEVMIGNAAQRFDAQGNL
                     TDETTKNLIRQLLQNLVAWTHRLAK"
     gene            15615..>16043
                     /locus_tag="VF182_18215"
     CDS             15615..>16043
                     /locus_tag="VF182_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439074.1"
                     /translation="MKPKNATAVEQRHRKSFFAQVKREMNGLYRSVRHQLAYYESVGD
                     LVPGDLAPYDVVDTVLLRGYPEFVKEPRDRDIGAWLKELATKHLQSEVTRSRTERSQN
                     VHLQEDIPETPPAEAVSTLGEEVLDFYQPDEDLKLEDIFRY"
BASE COUNT         3544 a         4871 c         4650 g         2978 t
ORIGIN      
        1 cgatcatgtg cgcggcgacc ggcgcggtta gaaaaagaaa tataacggtc gccaccgcgc
       61 gggtcgcaat gccgagctcg ccaaagtgaa cggccaccgc gagcatgata caaccaatcc
      121 ccaacgtcga agccttggtc gccgcctgca tgcgcatgaa caagtcgggc atgcgtacca
      181 ccccggcgcc cgcgagcagc ccgaataccg cgcctatgag catcagcgca gcgctgagcg
      241 cttcgctcat tgcgacacct gtttctcgag gtaacgcgcg aaggccacgg tcccgagaaa
      301 tgaaaccagc gcaagaattg tcgccacgtc gagaaaaacc gcttgcccgc tcgcgatagc
      361 atagatggcg gtgacgccgg ccgccaggat cgagatcagg tcgagagcca ccacacgatc
      421 gggcaagctc ggcccgcgta tcaggcggaa ggcggccagc atgatggcaa cagccatcat
      481 cgaaagggaa atgaaggata cgataaaaac ccagggcgcg gacattatct caaaacctcc
      541 aacactctgc gctcaaagct ggttttgacg tcttcgcggt attgctcgac gtcatcgata
      601 tacatcgcgt gcaaatatag aaaacgccga tcgctcgaaa catcgagact cacactcccg
      661 ggtgtcaggg ttatcaagtt ggcgagcatg gtaatttcgg catcgcttct ggcttccagt
      721 ggtatggcca gaacgcccgg ccgcgcacgc gcgcccggcg tcattacatc cgcggccacc
      781 cgcaggtttg ccatgacaag ctgccagaca tagaagaatg cgaaggcaat cgcctggtga
      841 atccttctga aatagttgga aggccccatg agcggttgca cgacgaacag aaccgcatat
      901 ccgaggacaa ttcccaccac gacattgctc agattaaagc gacccgtgac cgccgcccac
      961 acgaaggcaa gaaagatatt ccacagaaac atcttaaaaa ctcctctcca gcacgcgcga
     1021 gatatactcc gtcggatcca tgagttgttc ggcggcccgg ctggcgagaa gataaacggg
     1081 ctcgacagcg agcccgatgg tgatcgtaat cgccgccaga aagatgatcg gcgcgagcag
     1141 aatccaggct gcgccgcgtt tcgccagggc accgctcgct tccgcggcgt tcggacccgc
     1201 ggctgcttgc ttccaaaata cctccgccca gatctttgtc atggaaaaca aagtaagcag
     1261 gcccaccatg agagcggtgg caacaaggag atactgctca gcctgaagcc ccgcctgaac
     1321 aatcgtcagt ttggcaaaga atccggagag cggcggcaat ccggccagag aaagcgcggg
     1381 gaccaggaac aggattgcca agcccggatt tgacgagtaa aggccaccga gacttttgag
     1441 ttcaaatgtg ccgcgcagca ggtacacgac gccgctcaca agaaacagat tcgtcttaac
     1501 gatgatgtga tgaacgatgt aaaagatcga gccggcaatg ccggctgtcg taaacaaccc
     1561 gaggccgaga gtcatgtaac cgatctggct gataatgtgg aaggacagaa cgcggcggat
     1621 ttcgttctgc gccacggcgc cgagaacgcc cgtaaccatc gtcagcccgg acagcaccag
     1681 aatgatcgta tgcgtgtagc cgaggtcttg aacgaacaag agagtaaaaa ctcgaatgag
     1741 cgcatagacg ccgaccttgg tgagcaggcc cgcgaagatc gccgatacag cgacgggggg
     1801 cgtgtggtac gaagcgggaa gccagaaaaa aaacgggaag accgccgctt tgatcccgaa
     1861 ggcaacgaga aacagcatag cgattgccgt cagcagatcg aggcgcggag catctctcag
     1921 cttctgtgcc agatccgcca tgttcagcgt tcccaccacg ccgtaaagca tcccaaccgc
     1981 cgcgagaaac aaggcggacg ctatgagatt gatggtcaca tacttgatcg cgccttccat
     2041 ttgcgcccgc tccccgccca gcgccatcaa cacaaaagag gccatcagca tgacttcgaa
     2101 ccaaacgtat agattgaaaa gatcccccgc gagaaaagca ccgcacactc ccatcaacag
     2161 agcgtgaagg agcggataat aaccaaagcg ctctcgcgct cgatccacgg tcaccatcga
     2221 atagaccgcc accgacatac cggttatccc ggcaagcacc agcatgatgg cactgaaaag
     2281 gtcggcgacg atggtaatgc cgaacggcgc cggccaggcg cccacttgag taacctgaat
     2341 gccacccctt gagaccgatc gaagcagcat cagcactgaa cccagtaaca aggccgtccc
     2401 cactacgccg agtacccgct gggtgcgccg cgagcgccat gcgaacaatg agagcgtcgc
     2461 cgctgaaagc gggatcagca acggtaaaat caacagaacg ttcacgagtc cgtcgttttc
     2521 atttgatcca agtcatcgct gccgacaatc tgaaccaccc gttggatcag caccactgcg
     2581 aaagcttgaa cggcaaagcc gatgacgatg gcggtcaaga tcaacgcttg cggcatcgga
     2641 tcggcaaccg ctgacgcggt ggtgagaccg ccggtgacga gcggcggtct cgcccgggtc
     2701 aaccccgcga tggtaaagat gagcaaattg gcggcatgcc cgagcagact gatgccgatg
     2761 atcaacctga ccgcgctggg acgcagcatc atgaacagcc cggcggcata gagcgcgcct
     2821 atgacgaatg ccagtacggt ctccatcgct attcttccgc cagagtgaga atcaacgtca
     2881 ggaccacccc gagcaccaac agataaacgc cacagtcgaa gactagcggc gtgccgatat
     2941 cgagcttttc cagccccggg ataaaaacgc tgaaccattt accggtcata aacggttctc
     3001 cataaaagag agaaacgatc ccgctcacca gcgcaatcga gagccctgct ggaatcaaca
     3061 tccgggggtc aacgcgcaac gtgcgccgcg tgctgcgcgc atcgaatgca atggcattga
     3121 gcgaaaaggc cgcggcagcg atcaagcccc cgataaaccc gccgcccggc tcgttatggc
     3181 cacgtatcag gatgaacaat gaaaagagca gtagaagcgg cagcaaatag cgggcggcgg
     3241 tggaaaggat gagtgatgtc acgatgtctt ctccttgcgc ggcctcagcc tgaggagcgc
     3301 gtaaaccccg atgcccgcga ccgccagcac cgtgatctct ccgaacgtat caaattcccg
     3361 gaaatcgacc aggatggtgt ttacgacatt tcggccgtgg gcgagcgcca ggctattttc
     3421 gacaaagtaa ttcgatatct tgggatagag ttggatgccg gagccgaccg ctacgatgac
     3481 cgtgatgagc ccgccgaacg tcaatgcggt cacggcatcg cgcaccaaaa tcagccgggg
     3541 ggagaaagcg gtgaagcgcg gcaagtggta aaacaccaga acgaacagaa tcaccgtgag
     3601 cgtttccacc atgaactgtg tcatcgccag atccggcgcg ctgaaaagaa cgaaaatcaa
     3661 gccgacgctg taaccaacta ctccgagcgc cgcgaccgcg cctaaccgcg aacgcgacat
     3721 gatcgcggta agcgcgccga tcgggatcaa cgccgcgatg atccattcgt agaaccgcac
     3781 gtcgagctcc gccggcaaat ggatggagcc gatggcgcgg aacagtttgg ccgcgaccag
     3841 cgcgaccgtg gcggcaacga tgatcagcag ataaaagcgc aagtaaccgt tttgcaggag
     3901 ctgggtttgg cgtttcgcca gagcgttcat gccggccaaa agctgtccgt accaatgctg
     3961 cggtccccac caggaagtaa tttgcagacg cgcggcggca tcatggaccc gccgtcgtcc
     4021 ggcatagaga gccgcgccgc agagcagcga aagcgcgctc aggccgagcg gcaggtttag
     4081 gccgtgccag agggcaagat ggaaactgac gggttctctc atcacggcct gaaccccggc
     4141 tcgcacgagc ggggcttcga cgagatttgg catggcgccg aagagaatgc cgcaaacgct
     4201 caaaaccagg ggcgccaacc acaaagtgaa aggagcttcg tgggcgcgcg tcgcagcggc
     4261 gctctttcta ccgaaaaacg gccggatcgc gatcattgcc gcagcagcga caaacacgct
     4321 gcttgcggcc acgagcgcca tcgtcatcag tgccgcgccg gtttcggctc cgagcgaggc
     4381 ttccagcagc aattccttgc cgataaagcc gaaagccgga ggcaaagcgg cgagtgatag
     4441 cgccgccatc gcggctaggg ccgcggtgag gggcatcgcg gataacagac cgcgcaactg
     4501 ccctacgtct ctcgtaccgg tttcatgatc gacgattccg acgacgagaa acaaagcgcc
     4561 cttgtaaagc gcatgcccga gcaggaaaac gacagccgca atgatcgctt cggacgttcc
     4621 caacccgatg aagagaacca gaatgccgag ggcgctgacc gtcaagtagg cgaggatcaa
     4681 tttcagatcg gtttgtctga gcgcgaggta cgcgccgacg gccatcgtcg tagcgccggc
     4741 cgttgtcagc gccaacatcc acagttccgt gcctccaagg accggtgtta aacgcgcgag
     4801 cagatacacc cccgctttca ccatcgttgc ggagtgaaga taagcgctca ccggagtcgg
     4861 cgcgaccatg gcgcccggca gccaaaaatg aaaaggcacc tgtgcggatt tcgtaaacgc
     4921 cccggccagc acgagcagca cgatcggtcg atagagcgga tccgatcgga tcgttgcgcc
     4981 gctttccgcc agggctgaga tctccatggt ggcgccaata tggccgagca gcagaaaacc
     5041 ggcgagcaac gccaggcccc cgccgctggt caccagcagc gcctgcaagg cggcggcgcg
     5101 cgcatcttcg ctctcgtgat cgaaaccgat gagcaaatag gagctgatgc tcgtgagttc
     5161 ccaaaagacg aaaagtgtca tcaggttatc ggccagaacg atccccagca tcgatcccat
     5221 gaacaacaag atatagacga aaaagcgacc gagttgggaa tggccggcca gatagccgct
     5281 cgagtagatg ataatcaatg cgccgatggc gctaatcagg atcgcgaaaa gtaaactcaa
     5341 accatcaagg gaaaaagaaa agcggattcc tagactggga gcccaatcat aggaaaagag
     5401 gaagacttcg ccggaagaaa cagcggcgat gaaactgccg aagtacagcg tcagccctcc
     5461 cggcaagagg gcaagaaacc atcccgcggc gcggccgctc atccgataaa gccgcggcgc
     5521 cagaagcgaa gctgcgaaac ttgagaagat agccgctaac actcacgcct cctaagccaa
     5581 tcactaattt gaacggaccc agcttccgtt ctcgaatcta tcaaggtcga aaatcgattt
     5641 cgctggatgc cctacctgga ataatagcta cgggtcgtgg ttgggctggc cgcactccgg
     5701 aagtcaaccg tttctcatac gggtaaagag tttgctcaac tcgcgcagcc acaggaccga
     5761 gctcgccaca gccgcgcaaa cgagccaatc gccggccgtc aaatccatcg tcgaaaacgc
     5821 ttgctggaga aacggcgtat agatcacggc gacatggagc gcaagcgaca atccaatggc
     5881 tgcccacagc cagtggttat ggaaaagacc ggtaaacgcg ctctgcgcat cggaacgcgc
     5941 gttaaaaata ttgaaaagct gaaacatcat caaggtcgtg aacgccatcg tttgcgcata
     6001 gcgcgtcgtg ccggagccct ccaaaaaccc tcccggtagc gcgccgtcga gaacgcacag
     6061 cgttccgacg gccatcacag cgccgacgaa aaaaatcccc ccccacattc tgccggtaat
     6121 cacgccttcg ttgcgcgggc gcggcggttg gctcatcacg ccgggatctg ccggatccac
     6181 tccgagcgca agcgcgggag cgccatcggt cacgagattg atccacagga tctgggtcgc
     6241 gagcaacggg agcaccacgg cctgtccggc tgtggcggtg agcccgatga agtccgccag
     6301 caacacgccg aaaaacatcg tcatcacttc gccgatgttc gacgacagca ggtagcgcag
     6361 gaacttccgg atattggcaa aaatggcccg cccctcttcc actgccgcaa cgatcgtggc
     6421 gaagttgtcg tcggccaaca ccatatcggc ggcttctttg gagacgtccg tgccggtaat
     6481 ccccatagcc acgccgatgt cggcgctctt caacgcgggc gcatcgttga cgccgtcgcc
     6541 ggtcatcgcc accgtcgcgc cgcggcgttg caacgcatcg acgatgcgca gcttgtgttc
     6601 cggattgacg cgcgcataaa cggccacttc ctcaaccgtc cgctcgagct tgtcttccgg
     6661 catcttctcg agctcgccgc ccgtaaccgc tcgcccggtt tcagcgatgc ccagctcagc
     6721 ggcaatcact gcggcggttt tgggatgatc gccggtgatc attatcgggc gtatgccggc
     6781 cgccttggcc cgggcaacgg ctgctttcgc ctcctcccgg ggcggatcga tcatgccgat
     6841 caatcctgcg aagacgaggt tttgctcgac atcttcatcg acgttagctg cgtcgaacgc
     6901 ggccgccgga atggaacgaa aagccacgcc cagcgtgcgc agcgcctcgc cggccaacgc
     6961 ttcattgctc tgggcaattt ccgcgcgccg cgcgggcgtc agagggcgcg tttcggcgcc
     7021 gaccaactct tccgaacaac gcgcgagcaa gacatcgggg gcgcctttgg tgaagacgcg
     7081 cagacggtcc tgtgtctccg catccgtatg gacggtgctc atcaacttgc gttcggacga
     7141 aaacggaact tccccgacgc gctggaagcg ggcgtcgagc aggtggttgc gaaggccggc
     7201 tttccgcgcc gcgacaagaa gcgcgccttc ggtgggatcg ccttgcacgg tccagcggcc
     7261 gttgtgctcc tgcaacacgg cgttgttggc aagatcaccg gtcgcgagtg cgcgctcgag
     7321 ctcgatgcgc agcggcccct cgccgatggt gccgcccccc tcacgccata cttcaccctc
     7381 gggcgcatat cccgtgccgc cgatatttac gcggccgctc gcggcgatca cggcgcgcac
     7441 cgtcatttca tttttcgtga gcgtgccggt cttgtccgac gcgataaccg ttgcggagcc
     7501 gagcgtttcc accgcggata aatggcgcac gatggcattc cgcttcgcca tgcgctggac
     7561 gcccagcgcg agcaccgcgg tcacgaccgc aggcaagcct tcggggacgg cggcgactgc
     7621 cagcgccacg cccagaatga gcacgtctaa gatcgccgcg aagctgctga catcttcaac
     7681 gattataatg gtcgcgatca tcacgaccgc gatgatcacg acaatgaacc cgagcagttt
     7741 gccgacgcgg tcgagctcgc gttggagcgg cgtggtctcc tcgggcgctt ctttcaacat
     7801 cccggcgatg cgccccattt cggtttgcat cccggtcgcc accacaaccg ctttgccgcg
     7861 accgtacgtc gcagcggtgc cgctgaatac catattgccg cggtcgccga ccccgacctc
     7921 ttctatgatg ggagcgatat ccttggacag cggcaggctc tccccggtga gcgccgcctc
     7981 ggcggtctgc aaagcggtct cctggatcag ccttgcgtcg gcgggaatcg tgtcgccctc
     8041 ttcgatgagg atgatgtcgc ccgggacaag ttcggcagcc ggaatgttct gccgctcgcc
     8101 gtcacggatg acattggcat gcgccgccga catgttgcgc agcgccgcca cggcctgttc
     8161 cgctcgcgcc tgctgaaagt agcccatcac ggcgttgagc agcaccaccg cgagaatcgc
     8221 gagcgcttcg taaggcaaag cggtgtcgcg ctcgagaagc cacaagcctg ccgagatcaa
     8281 ggtggcgagc agcagcagga tcaccagtac gtcttgaaac tgcgcaagaa actttttcca
     8341 gccgggcacc ggcttttctg ccgtcagctc gttcctgccg gacttcgcga ggcgtgttcg
     8401 cgcctccgcc gaggtgagac cgcgctgagc atcgccgacg taagctcgca ggacctgatc
     8461 gacagactgc cggtaaggct ccgtctgaga gggggccttc tttgccgaac cggtgggtgt
     8521 ttcactaacg ctcatgaagt cagtatcagc acgaagagca cgaagatcac gaagttccga
     8581 cggagaagat ttttcggatt acaacaagag ggcgaacgaa aattcaatca aggatggtgc
     8641 actctgccct ctatgctggt ccaccgccca gccgcttgga tacttccttt ctgattatcc
     8701 ttaaactttc gtgtgcttcg tgagcttcgt ggtgttcgca ttcaggatcc aggatcggag
     8761 ggtcatggag agttttgcat caaagactcc gcgctctgcg cctctgcggt atgcggtttg
     8821 cgttttagcg gatcgcattc tccattattc cagcttgaaa acccgcgtgc cgccggcaaa
     8881 ttcataaatc tcatctcgga accccaaccg gatgggcgcg ccgccccgct cgcgttcccg
     8941 aaccgtaagc gtatcgcggg tgagccgcag atccagtgaa tggctgcggt agcggatgcg
     9001 catgtcgaac cgttccagtt cgtccggcag ccggggattg aaccgcaaga catcgccggt
     9061 gatctcaatc cccgtcgaca cgcgctgcac caaatcgacg cttcccgcca tggcgccgag
     9121 atgcacgccc tccgccgtgg tgcctccctg aatgtcgttg acatcgctct gcagcgcctc
     9181 ggcgaagtaa cgcatcgcgc gcggccggtc cgagcgcgcc agcacccatg catggaccac
     9241 ccgcgacaaa gtggagccat gactcgagcg gttatcgtaa tacgcgacgt tacgggggat
     9301 cgtgtcatac tcgaatggat aggccaggcg ttcgaacagt ccgcgcagtt cctccgcgga
     9361 aaaaagatag aacaacatca gcacatcggc ctgcttgccc actttgtagc ggttggcgct
     9421 gtcgttctcc gcttcgagaa tcagttcgag ccgctgaatg ttgccgtagc gcttgcggta
     9481 atcctcccaa tccagctcct tgagccgctc gtagccctcg aactggctga tgatgccgtc
     9541 gtgaaaaggc acgtacattt tacggctcac gtcgcgccag tgctcgagtt catccgctga
     9601 cagccggtta cgcgccatca gctcgttgcg ccggatatcg gtgagcagtt cgagcacgtc
     9661 gaaggcgcgg caaaggaccc ataccgccat gatgttggta taggcattat tgtcgaggcc
     9721 agggctggat gagtccgggt atccgtcatg aaactcgtcc ggccccatca cgccgcgaat
     9781 ttcgtaacgc ccgcgctcag gattgaaagt cgcgacgctc gaccagaagc gcgcaatttc
     9841 aatcatcagc tcggcgccgt aggcctgcag gaactcgatg tcaccggtga cctgatagta
     9901 ctgccatacg ttgtacgcga cggcgctgcc gacgtggcgc tggaggtaag tcttatccgg
     9961 tacccaacga ttggaccggg gattcaaatt gagctcctga gtttcctcct gtccgtcgct
    10021 gccgctttgc cacggaaaca tggcgccccg gaatcccgcg ttcgccgccg cagcgcgcgc
    10081 ctcgtccaaa cggcggtaac ggtacatcag cagggagcgg gtgatttcgg ggacacggaa
    10141 atttaggaac ggaaaaatga agagctcatc ccaaaacacg tggccctggt aagcctcccc
    10201 ggtccagccg cgcgcgggca caccaaggtc gaggccaatg gagttgggcg acgctgcctc
    10261 aagcaagtgg aacatgctca gccgcaggag catcgagacg ttgagctgga agccgctggg
    10321 agcgccgcgc aagtgaatat cgaaacgtcg ccacagatgt ttccaggcta gcgcatgttg
    10381 ggcgagcaag gcttcaaagc gcggcgcgcg cccgatggcc ttgcgcgcgg cggtcgccgg
    10441 ttccgagatc gccggatcgc gcgagctgaa gatcgccgcg atcttctcca gcgacagggt
    10501 ttcgccccgg cccagatgga gcgtcaattc ttgggcgacc ttgccgggct cgtcgatgag
    10561 atgccggcgg tactcgcgcg gctctccgcc gacgaatgcc tgggtgcgcg ccgattctgc
    10621 gacgtgcaaa ttcgactggc aggttcgcac caggagatac agcgtgtcct cgccaatgag
    10681 cgcgccgcca agatgttcca ggtgtttgtt gttgaatttg aggtagagtt tggcgccggc
    10741 gttgacgact cggccattga tacccgagcg tacggtcacg ccgccgaacc agttatcggc
    10801 ggtcagcgac agctcgagtc cggcggtgtg catatcggcc atggaaacaa accggcgctc
    10861 tcggagcgtg gtgcgcctgc cgccgcgatc ttcaaaacag atgctgcgca aaagcacgcc
    10921 gcgccggaga tcgagttcct gacgatacga gagaatgttc acccttctca agtcgaacca
    10981 ttcgccgcgg tcaatgcgga actcgagcgc gagccagttg ggaaagttaa ccagatcctc
    11041 gttttcgacg atgcgtccgg cgatatcggt gcgctgccgg ttgtaaccgc atgcgagata
    11101 ggttcccgga tagtggacat catccatcac ggcccaggcg gccgctcccc gcgtggcgaa
    11161 atatccgttg ccgagcgcgc acaacgcttc gcgaatccct tcctgctccg gatcgaagcg
    11221 gtcgtaaacc agcgaccacg cggaagccgg ttcggtggcg atctgcacct cggccagact
    11281 agttaccacg atatcggcgc cggcctggcg caactcctgg gattggccgg cgagatcgac
    11341 gccgatcacg cagccgaatg tcccggcgcg cccggcggcg acaccagcga tcgcatcttc
    11401 aatgaccacg gcgcgagccg gttccacctt gagccgccgc gccgcttcga ggaaggcatc
    11461 gggcgcgggc ttgcccctga ttttcatgcg cacaatgtcg ttaccgtcca cgcgcgtgtc
    11521 gaacagttgc gcgatgtccg ccgccctgag aacagccgcg caatttttac tcgaggaaac
    11581 caccgcagtc ttgatttcca gctcgcgcaa agcgcgcacc agctcgatgg ccggcgcgta
    11641 aacctcgacg ccgtgctgct cgatctgctc tgaaaaatag cggtcctttc ggttgccgag
    11701 tccatgcacc gtctgtgtgt cgggcccgtc tgcagcagag ccgagcggca atccaatgcc
    11761 ccgagcgttc aggaacgatg cgacaccgtc gtagcgcggc ttgccatcga cgtaacggcg
    11821 gtaatccgca tcgatgtcga acggcgcgaa tgcttctcgt gtctctgcgg catgctgccg
    11881 gagaaattcg tcaaacaact ttttccatgc ggctgcatgc acgctcgctg tcttggtcaa
    11941 gacgccgtcc agatcgaaca aaaatgcgtc gtaatcccgt ggcgagagcg tgacgacggg
    12001 ggttgtacta gtcatgggct attctctggt gtgaaatgga atccggaatc caaatatcga
    12061 aatccgaaac aatctccgaa attcgaaata aaaaagctga ccgccgtgtt tgaaccttgg
    12121 tattttatta tttgagtttg tttcgagttc ggatttcgtg cttcgaattt tccgccaggg
    12181 cggtggcgcc gctaggaaac tctccttacg gaacctcgat catgcgctga atcatgagat
    12241 ggggtgacga gcgctcccgt ttccatcagc gggcaacatt cgagtctcga gaggcggtgc
    12301 cggcggaagc ccggtgactt cgagaatttc cttttcgttg agagtctccc gcgccactag
    12361 ggcttgggcg agcgcatcca gttgcttacg gtgcgcggtt aacaaacgct tggcttcttc
    12421 gtggctctcg ccaatgatct tgagcaactc ggagtcaatg gcttcggcgg tctcttcgct
    12481 aaactgcctc gcgccgccgt aaccgtccgg gccacgcaga tagggattgt cgcgcggagc
    12541 gagctgcacc aagccgagcc gctcgctcat gccccagcgg gtcaccatgc gccgggcaag
    12601 acccgtagcc tgctcgatgt cgctttcggc gccagtggtc ttggtaccgt aaacaatctc
    12661 ctcggcggcc cgacctccga gcatgccgac gatgcgcgcg cgaagatagg cttcgggata
    12721 attgtagcga tcgctgtccg gccgctgata ggtcacgccg agcgcctgcc cgcgcggtac
    12781 gatcgtgacc cggtttaccg gatcggcgcc gggcacgacc aaaccaagaa tggcgtgtcc
    12841 gccttcgtga taagcgatac gctctttgtc agcgaggctg agcagcagtg ggcgctcggg
    12901 acccagcaca attttctcga gagcatcgag aaagtccttc tgacggacat cgctctgctc
    12961 gcgccgggcc gccagcagcg ccgcctcatt caccaggttt ctcagatccg ctccggagaa
    13021 ccccggtgtc gcagcggcaa gctcttccaa gcttgcatct ttggcgagcg gcacggtgcg
    13081 ggtgtgaact ttgaggatcg cctcgcgccc gatcttatca gggaggttga cgacgacgcg
    13141 ccggtcgaag cgcccgggcc gcagcagcgc cttgtcgagc acgtcgggct ggttggttgc
    13201 cgccagcacg atgatgccct cccggcttga gaagccgtcc atttcagtga gtatttggtt
    13261 taacgtttgt tcctgctcgc tcgatccgcc gatcgctacc tgtccccgcg cgcgcccaat
    13321 ggcgtccaat tcgtcgatga agacgatggc gggcgcctgc tcgtgcgcct gcttgaataa
    13381 atcacgcacc cgcgccgcgc cgactccgac gatcatctcg acgaactccg cggcgctcat
    13441 cgagaagaac gggacgccgg cctctcctgc gactgccctc gcgagtaggg tttttcccgt
    13501 gcccggagcg ccgaccagca gcacgccttt gggggcggcg ccaccgagcc gggtgtactt
    13561 cttgggatcc ttcaggaaat cgacgatctc gaccagttcg ttttcggctt cgtcgatccc
    13621 ggcaacgtct tcgaaggtaa ccttggtatc cttttcctga tcgtagcggc gcgccttgct
    13681 cttgccgatt cccatcagcc cgcccatgcc gccgccttgc tgcgccgcgc gccggaacat
    13741 ccagacgtaa aagccgataa agagcaaggc tgggccaaac ccgaagagca gcgtagataa
    13801 cgggccaccg ctttcgtcga tcggtttggc gctgatttcg acaccgttat cgatcaaaaa
    13861 cgattccagc cccggatcga caaaggccgg cagtgtagtc gtgaagttag ttgccgtctt
    13921 ggtagcaggg cgcggagtcg caccccgttc tttgggctgt ttggtttctt tggcgggagc
    13981 ctcacttttt tcctccttag gagggtaggt gaccgcagtc ttgaagcggc cggtgatgga
    14041 ttccccctgg ctgtagatcg attcgacatt gccctttccg acttcctctt tgaacagcgt
    14101 atagggaacg gtgaccggcc cttccgggct cggaatgaga agtctcatca gcaaatagtt
    14161 tgcgagtaaa atgcctacga accagagcca tgttctaccg ggcggcatct tccactgctc
    14221 gccactcttc gaatctccag gcttattgct ggacgactga agacggctgg ctgcgccgga
    14281 gtcgccgcgt cctgtctgct tccccgtcga attcatggaa cccccctatg aacgttgccc
    14341 gaccgcaggc agaatcgttg cagatgatga ttgacgacta tcattcacac cgaatgacaa
    14401 caagattaat ttctgctttt gctcggatcg gtgccccggc ggtttcgccc gggcacgagt
    14461 gttggactct ctgatggtgg cctgctcatt gttcggcttt tttgctggcc caactttaga
    14521 agtaattcta actccactcg cctccgctgc gctcacggag ttgcgcccgt aagacctacc
    14581 ggcccggcct ggtcaaagtt cgttgctgcg ccgtattcac accaacgggt taccttgatt
    14641 ggtccgttca tagttgtagg gtcaaatata aggacagagc gccagagtga tgctccccaa
    14701 ggtttactcc aaagttcaat cggtgatcgt gaccagactc aggtggcgtt cgattcccgt
    14761 tctattcctc cacgtcgata acgcggaacg aagattgtga ttgaatgcct gtaaggcatg
    14821 tagcaactgt ctgaaacata agaagcgaat caaccaataa actcgtctac cttattagga
    14881 gaacacaaat gagcaacccc gttcgcattc tcggcattgc cggtagtctt cgtcgtggat
    14941 cgtacaatcg agccgcgctg cacgccgctg tgcagcttgt gccgcaaaac gcggcgcttg
    15001 aagtttttga gctcgatggg atacccggct tcaatcagga cgaagagcag aacccgccgg
    15061 caaaagtcgc ggagttaaag cagcatgttc gaaatgcgga tgcgattgtg atcgtcacgc
    15121 cggaatataa ctactccgtt ccgggggttc tgaaaaacgc aatcgactgg gcatcgagac
    15181 cgtacggcga cagcgcctgg aacggaaagc cggttgccat catgggcgct tcgattggaa
    15241 cattcggcac cgcgcgggcg caataccatc ttcgccagtc attcgtcttc ctcaatatgt
    15301 ttccgatcaa ccagccggag gtgatgatcg gcaacgccgc tcagcgtttc gacgctcagg
    15361 gaaaccttac agatgagacg acgaaaaacc taatccgtca gctgcttcaa aatcttgtcg
    15421 cttggactca tcggctcgca aaatagagta ggggacccga agccagtcgg tgatgtagaa
    15481 atctgttttg gcgccaagcg gcaatgcgca tatcgcggtt tagagggtgg cgataaaacc
    15541 accgaagcgg ctcggacctg gctgcatcag cgatgcaatg caagcggcgc atccaaaacc
    15601 atggagcacg ggaaatgaaa ccgaagaatg cgacggcggt tgaacaacgt catcgtaaat
    15661 cgttttttgc tcaggtgaag cgcgaaatga acggactcta tcgctccgtg cgccaccagc
    15721 tcgcctacta tgaatcggtt ggcgatttag ttcccggcga tttggctccg tacgacgtcg
    15781 ttgataccgt gctgctgcgc ggttacccgg agtttgtcaa agagccgcgc gaccgagaca
    15841 tcggcgcctg gttgaaagaa cttgcgacaa agcatctcca gtcggaagtg acccgatcgc
    15901 ggacggaacg gagccagaat gtccatctgc aagaagatat tccagagacg ccacccgcag
    15961 aagcggtctc gaccctcgga gaggaagttc tcgacttcta tcagcccgac gaagatctga
    16021 agctcgagga tattttccgc tat
//
LOCUS       DASUVS010000341         3591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1674402, whole genome shotgun sequence.
ACCESSION   DASUVS010000341 DASUVS010000000
VERSION     DASUVS010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674402"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VF182_18220"
     CDS             <1..633
                     /locus_tag="VF182_18220"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6439075.1"
                     /translation="AITQLGAIGLCLVPEPAKGRHIHDRYYDPVWREAEKLGVPVCFH
                     PAANPNTDQVVHRFKGHANEAVLINAFRNPIELMLAVGSFCAGGVLERFPRLRVAFLE
                     GNCGWLPWVLYRLDERWELRKGYTNEPLSTKPSDYFIRQCFISVDVEEDLVGDVINRI
                     GENNVVISTDYPHADSHWPNAVNHFMAMEMPSSARKKILWDNCARLYAVD"
     gene            complement(665..893)
                     /locus_tag="VF182_18225"
                     /pseudo
     CDS             complement(665..893)
                     /locus_tag="VF182_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6429 family protein"
     gene            complement(959..1993)
                     /locus_tag="VF182_18230"
     CDS             complement(959..1993)
                     /locus_tag="VF182_18230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6439076.1"
                     /translation="MKFGYYLLNTYVPELDGESPELYARWLEQMDAAEDLGFDSLWVT
                     EHHFRYFGGMMPSPSVLLAAAAQRTKNMRLGAAVSILPMHNPLRIAEEFAMVDLLSNG
                     RAMLGAGRGMHPTEYKVFSAEWETAQQRLPEALDIVIRAWTDGEFEWAGKHYSFPKIA
                     VYPKPMQKPHPPIYVTANKDPESFQMIGSRGHHLMTLPWIATNEIQRPRVEQYWSALS
                     AAGHSKKDRDVFVMYPAYIGASDAEARAEVLDHWNRWRGFALSALNLDPDSEAYQRVF
                     AHLDYDAMSRDSRGVFGGPETCAKIVNRIIEVVGATHIGLTFHFGGLSQEKVLASMER
                     FAQRVRPALG"
     gene            complement(2113..3144)
                     /locus_tag="VF182_18235"
     CDS             complement(2113..3144)
                     /locus_tag="VF182_18235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6439077.1"
                     /translation="MKIDAGLLVHDLRQMPALARFTDEAGFDGLWTFETAHEPFLPLV
                     LAAEHSRDLSLGTSIAVAFARSPTVLAHTGWDLARFSNGRFIMGLGTQVKGHNERRFG
                     VKWEKPVEKMREVILGLRAIWDCWQNGTKLNFQGEFFKLTLMTPFFSPEPHEYNRIPI
                     YIAGVNRRMCQLAGELCQGFHVHPLHTSRYLREIVTPNIERGLTTGGRDRKAIELSSS
                     IFVVPTDDPGSIERYEAEIRRQIAFYASTPPYRPVFEIEGWGETAQQLGRLAAKGRWN
                     DMPLLISDRMLEAFALRGSWSELPAKVLEKYHGLLDRVSYYFPVVPGENEDGWRATVA
                     GFKAPADRR"
BASE COUNT          814 a         1021 c          968 g          788 t
ORIGIN      
        1 gcgattacgc aattgggggc gatcggtttg tgcctcgtgc ctgagccggc taaaggccgc
       61 cacattcacg atcgctatta cgatcccgtt tggcgcgagg cggaaaagct cggagtgccg
      121 gtttgtttcc atccggccgc gaatcccaat accgaccagg tggttcaccg atttaagggg
      181 catgccaatg aggccgtgct gatcaatgcg ttccgcaacc cgatcgaact catgctggcg
      241 gtgggcagct tctgcgccgg cggcgtgctc gagcgcttcc ctcgattgcg cgtcgctttc
      301 ctcgagggga actgcggttg gctaccctgg gtactctacc gcttggacga aaggtgggag
      361 ctcaggaagg gctacaccaa cgaacccctc agcacaaaac cgagcgacta cttcatacgc
      421 cagtgtttta tctcggtgga tgtcgaagaa gatctagtgg gtgatgtcat caacaggatt
      481 ggcgagaata atgtggtgat ctccaccgac tacccgcacg cggactccca ttggcccaat
      541 gcagtcaatc actttatggc gatggaaatg ccgagcagcg cgaggaaaaa gatcctgtgg
      601 gataattgcg cgcggcttta tgccgtagat taaacgtacg ccacggaaga agaatttgga
      661 ctaactattt ctcgacgaaa agcttggtaa ataagcgttc ggattcgcgc agcccgtctt
      721 ccgttaacct cacggacttc gctttgcgga caggattaga gataaacccc tttttcacgg
      781 agccggctca ttgcgtccca atcaaagctc ttccaagccc taatttcgtg aaatgtcaga
      841 taaagtagcg cgagaactgc ctcatcgatc ttttctgtag cgaaatcctt tatcccatgc
      901 ctttaagcgc agaggcgacg cgcagcgcat tagtaaaatc cgtttcggtt cggcaatgct
      961 atcctagcgc cggcctcacc cgctgtgcaa accgttccat tgatgcgaga accttctcct
     1021 ggctcaaacc gccgaagtga aacgtcaaac cgatatgggt ggcacccaca acctcgataa
     1081 ttctgttgac gatcttggcg caggtttccg gcccgccaaa aactcctcgg ctgtctcgag
     1141 acatggcatc gtagtccaaa tgggcgaata cgcgctggta ggcttcgctg tcgggatcga
     1201 ggttgagagc agagagcgca aaaccacgcc atcggttcca atgatcgaga acttcagcgc
     1261 gggcttcggc gtcgctggcg ccgatatacg cggggtacat gacgaacacg tcccggtctt
     1321 ttttggagtg gccggcagcg gatagcgcgc tccaatactg ctcgacacgc gggcgctgga
     1381 tttcattggt agcgatccat ggcagtgtca tcaggtgatg gccacgactg ccgatcatct
     1441 gaaagctttc cggatcttta ttggcggtga cgtaaatcgg cggatggggt ttctgcatcg
     1501 gcttgggata aacggcaatc tttggaaagg aatagtgctt gccggcccac tcgaattctc
     1561 cgtcagtcca cgcccggata acgatatcca aggcctccgg taatctttgc tgagcggtct
     1621 cccactcggc gctaaagacc ttgtactccg tcggatgcat gccgcgtccg gcgccaagca
     1681 tagctcgccc attggagagc aaatccacca tggcaaattc ttcggcgatg cgcagaggat
     1741 tatgcatcgg tagaatcgag actgccgcgc ccaaccgcat atttttcgtg cgctgggcgg
     1801 ctgcggcgag aagcacggag gggctcggca tcatgccgcc gaaataacga aagtgatgtt
     1861 cggtcaccca taaagaatcg aagccaagat cttccgccgc atccatctgc tcgagccagc
     1921 gggcatagag ttcagggctc tcgccatcga gttccggcac ataagtattt agaagataat
     1981 aaccaaactt cacaatgacc tgacctctcg cgagtagctt cccggaagat tacgttatcg
     2041 acggttcggc ttacgctctc gacaatcccg aacatggcgc tgagaacggc atcatgactg
     2101 cctcaaaatg agctagcgcc gatccgccgg cgctttgaag ccggcgacgg tggcccgcca
     2161 gccgtcttcg ttctcgcccg gcaccacagg aaaataatag ctcactcgat ccaacaagcc
     2221 gtgatatttt tccagcacct ttgccggcag ctctgaccag ctaccgcgca gcgcgaaagc
     2281 ttcgagcatc cgatcgctaa tcagcaatgg catatcgttc caccggcctt tcgccgccag
     2341 gcgtccgagc tgctgggcgg tttcacccca gccttcgatt tcgaaaaccg gccggtacgg
     2401 gggagtgctg gcataaaacg cgatctgccg gcgtatctca gcttcgtatc gctcgattga
     2461 tccgggatca tcggtgggga cgacgaagat actgctcgac agctcgatcg ctttacgatc
     2521 gcgaccgcct gtggttaaac cgcgttcaat attgggcgtg actatttccc gcaggtagcg
     2581 gctagtgtgc agcgggtgaa cgtgaaagcc ctggcataat tcaccggcca gttggcacat
     2641 gcgccggttg acgccggcga tgtagatcgg gatgcgatta tattcgtgcg gctcggggct
     2701 gaagaacggc gtcatcagag tcagcttgaa aaactcgccc tgaaagttga gcttcgtccc
     2761 attctgccaa caatcccaaa tagctcgcaa gccgaggatt acttcgcgca tcttttcgac
     2821 cggtttttcc cacttgacgc cgaaacgccg ctcattgtgt cccttgacct gagtgccgag
     2881 tcccataata aagcggccgt tggagaaacg cgccagatcc cagccggtgt gggccaggac
     2941 ggtcggactg cgggcgaatg ccacagcgat actcgttccc agactcaagt cgcggctatg
     3001 ctcggcggcc aacaccagag gcaagaaagg ctcatgggcc gtttcgaatg tccacaaccc
     3061 atcgaaccct gcttcatctg tgaaacgcgc cagcgcaggc atctggcgca gatcatgaac
     3121 cagcaggcca gcatcgattt tcatcgttta catcccaatt tgacactgct ccaacttagc
     3181 aacaaacggc atcgacgaaa atgctcggct actgaaagat tccgagtagg ctccgggtgg
     3241 cttgcatccg cgcgacgacg agactgcccg tttcctgcag gtgaccacat aggcttcgtt
     3301 gatcgtgatt cggatggttt tcattagccg cccaatttgc ctcgggcttg tttcaaaccc
     3361 gcaccaaaga ctagcagggt gctaaaaaaa gcctcttgca ggctgctcga aaagatccag
     3421 aggcgagacg cgcgaaaaat cgacgagcgg agactttcta tgagccctgt caagcctcga
     3481 tcggtttttt tcacgccgcc tggcgcaatt tctgtcgtgc aatattcacc aagtgatggg
     3541 tttcgtcata aggtttatga tctctccaca tcacaaaaat aatcttcaac c
//
LOCUS       DASUVS010000342        11968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1892345, whole genome shotgun sequence.
ACCESSION   DASUVS010000342 DASUVS010000000
VERSION     DASUVS010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11968
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892345"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            887..2563
                     /locus_tag="VF182_18240"
     CDS             887..2563
                     /locus_tag="VF182_18240"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HEX6439078.1"
                     /translation="MAVKFAITLTTKPKARLMSQINVTDRNQQTFPASKPTQLHVRLF
                     CTILLSILLSGAAYPQESNTLSRPRGWALSQRAAPVPSFNEGRFTLFPELRSSGAPLL
                     LDKRSLLAQTINAPAPVAASSNLTTPVPQWGTEKSYLIPALEIVGFDVLLNLFDRVYY
                     GCCDYDVDFSSIKRNLHRGWNEDRDEFTVNQLGHPYQGSMYHGFARASGLNYWQGLAY
                     TFVGSLFWEIAGETTPPSRNDQISTGIGGSFLGEALFRMSNFWLEQGRGSPFWREVVA
                     ALISPPVGFNRLAFDQRFDGIFPSKDPEYYSRMHFGLVSATQDRRGGSGEIDRHEGIV
                     DVALDYGLPGKSGYTYDRPFDYFTFQAAASTAIGFESVSTYGLLLGKSYDLGKDYRGI
                     WGLYGSYSYMAPQIFRLASTGLSLGSTAQWRVFDYLAVQGTGLLGIGYASVSDIAGIS
                     DERDNHYGVAPQALLSLRLILGERASIDLTAREFFVADVSGSGVRHDNVVRTDASLTY
                     RIYRQHAVSIRYQLSRRDFDLRDTGNRIQDRGTVGIFYTLLGRDRFGAGD"
     gene            2911..4857
                     /gene="acs"
                     /locus_tag="VF182_18245"
     CDS             2911..4857
                     /gene="acs"
                     /locus_tag="VF182_18245"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX6439079.1"
                     /translation="MADKAIEVLLNEKRNFPPPATFRKTANFRNASVFNSARRNPQAF
                     WAKAAKELHWFKPWKKVLEWKAPWAKWFIGGKLNASYNCLDRHVENGRKNKAAIIWEG
                     EPGDERVLTYRDVWREVNKFANVLKKLGVRKGDRVCLYMGMVPELVIAMHACNRIGAP
                     HSVVFGGFSAEALRGRINDAQAKILITADGGYRRGGIVPLKENVDEALIDTPSIEHVV
                     VLERIGRESGAQMKSGRDLWWHDLMAEAPLWCEPEQMDSEDILYILYTSGTTGKPKGI
                     VHTTGGYLTGVNLVNNWVFDLKEDDVFWCAADIGWVTGHTHIVYGPMANGATQVMYEG
                     TPDWPDKDRLWRLVEKYGVTIFHTAPTAIRTFMRWGTEFPAKHNLKTLRLLGTVGEPI
                     NPEAWVWYHKYIGGNRCPVLDTWWQTETGHPLITPLPGITKLKPGSATLPFPGIEADV
                     VDENGKSVPKGGGGYLVLTKPWPAMMRTIYGDPDRYVQNYWSRFPGRYFAGDGCKRDK
                     DGYFWLLGRVDDVMNIAGHRISTMEVESALVDHQKVAEAAVIGKEHDIKGQAICAFVS
                     LKAGVDGGNSLLDELKNHVVKKIGAIARPDEILFAAELPKTRSGKIMRRLLRDIADGR
                     ALGDTTTLADANVVAKLKEQYAEE"
     gene            4862..5923
                     /locus_tag="VF182_18250"
     CDS             4862..5923
                     /locus_tag="VF182_18250"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439080.1"
                     /translation="MRVVQSKLHSRTISIGTSTIGGMRRHFTLWIMLSLLWFPSLGDA
                     QQREKVRVALGSISVNSSVIPIGAQHGMFAKYGVDLEPIYFGGGMNSIAALTSNSVQL
                     LAAGSTATIGARVGGIDITMFAVQSNKLDYSVMVAPDVKTPQDLKGKIVTGTRTGASA
                     DTALRLYLQRHGLVPDKDVVFIAVAESQQGRFNALVRSVVAGTVLPPPYSTVAKQQGF
                     RELADLRKTDIEYSGTSIAGIGSYVKAHPAAIEGFLKGYIESLNFFRTEKEKSIAGIM
                     KYLRISDRSRAEEGYEYYVDMMPVMPYASNAGVKAVLQFLGTRNPKALTANPEDFYDH
                     SFLKKIEESGFSKQFVARR"
     gene            5965..6975
                     /locus_tag="VF182_18255"
     CDS             5965..6975
                     /locus_tag="VF182_18255"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439081.1"
                     /translation="MEKMSAGRIFLIAFLAIALAGIGSLEAQDKPLKKISWGVTSLSA
                     SNWIPWIAKDAKIYEKHGLDVELVLVKGSGQTSTAILGGSLFAAPVAVPQVMLADLAG
                     ADLVNIAHTVPGVQSKLLVKQEIKRPEDIKGKRIATSSIGSLGDFLFKYIIRKNGMDP
                     MRDVIWLSIGTPQERLQALSSGRADAADLSYPIDAQALRLGYHVLWDARKEVVYPSMS
                     VVTRRKTVQDDRDTVMRMLKSHVEAIAYFKAHKDFSMKVLGKYLRTNDQELLEGSYEI
                     FRQDFISVPYPIMNGLEATYDYVAQTRPEIRSRKPEEFMDPSLMAELDKSGFIKKLYA
                     QK"
     gene            7157..8245
                     /locus_tag="VF182_18260"
     CDS             7157..8245
                     /locus_tag="VF182_18260"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6439082.1"
                     /translation="MRLSSKLLASIFLLGIAFAGLKMPSARAASVEEIALLNKPDRQK
                     ILMEGAKKEGKISWYTSLIVDQVVRPVTAAFEKEYPFIQVEFFRANSERIVQRMFSEY
                     QAKRYDVDLLDGTVTAAMIRRANFLQRFHSPYLAEHSAETRDPQGNWASTNVYFFALG
                     YNTRMVKPNEVPKTYEDLLNPRWKGQMMWSTSRGSGAPMFIGNVLQSMGESGGKAYLQ
                     KLKAQNIAKSTASNRQLLDLVIAGEYPIALHIFNHHAYISKTAGAPVDWQALEPVTAT
                     LNTIALASRAPHPHASMLFLDFILSERGQKVFQQVNYLPSHPKVPAKQPDLKPGGGRF
                     NKALYITPEAQFEKGNDWVDYFNKQFMN"
     gene            8586..10364
                     /locus_tag="VF182_18265"
     CDS             8586..10364
                     /locus_tag="VF182_18265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6439083.1"
                     /translation="MNRQQSTEINLSAQSATVPAPRRVFPLSMGSRWGIESWVMIVVT
                     LITAYLVLPPLYSVIQTSLFTTTFTGELDQFTLRYYEELLRELRVLGPFLNTLYFSIG
                     SALLATLIGGMVAWVVTRTDTPLRGLGYFTAFASFGTPFILYTIGWILLLGKAGPVNH
                     WLKILFNQNTPVINVYSMAGMIFIEALLWSPFVFLMLAASFRSMDPSLEEASAACGAR
                     VWQTLRRISLRLMLPAFFSVLLLIFIRAFESFEIPALVGLPGDIRVLTTSIYQDAQRL
                     PPQYGNAGAFAVLLMVVVAATLYVYFRATRDGDRFHTVTGKGYRPALIRLGRWRYVSA
                     AGLLFYSFVLLVLPFLIILWASLLPFYIQPSLEAISKFTVKNYLLAIHFPKIIASIKN
                     SILLGLVSATMVMVLTTLASWILVRSKLRGRWLLDLLTTLPLLFPGIVMGLAIMRFYL
                     NVPIPIYGTLWILLLAFVTRYIPYGIRYTHSGLLQLHKELEEASYVSGASWVNCMRRI
                     TLPLIMPSFLGGWVFVFLLSAKELSMSILLVSPQTPVVSVAIYELWENGQVGELAAFS
                     VIWTAILVSVAVSYYLFARRFGVQQD"
     gene            complement(10572..10877)
                     /locus_tag="VF182_18270"
     CDS             complement(10572..10877)
                     /locus_tag="VF182_18270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439084.1"
                     /translation="MDYGPPRTLEDFKAEQRRRRWGYVLMPFFTLASIVVSVGMLMFF
                     GKACQDRIPADAPLRTSPLFKQYQQPQKQQGGVEDPSRAPEPSPAENRLPTRAEKSQ"
     gene            11323..>11968
                     /locus_tag="VF182_18275"
     CDS             11323..>11968
                     /locus_tag="VF182_18275"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX6439085.1"
                     /translation="MSKTAGIIIIGNEVLSGKTQDTNSHFLCVELRRLGVEVQKISTI
                     QDEIDLIGQEAAYFSKRFDFVFTTGGVGPTHDDVTIEGIAKGFSVPVVRHPEIEHRMR
                     QRLGGDVNQARLRMANVPEGAVLLATDALFAPVVNIHNVYIFPGIPRILQERFHAIKE
                     RFRDAPYFLKNVFVRYGEGVIASALNNLLAEFPHLLLGSYPVLDLPDYKVKVTLE"
BASE COUNT         2619 a         3187 c         3324 g         2838 t
ORIGIN      
        1 tgtcttagtc ccggcctgtg cgcccgccgt ccagcgtcga gcctcgattg cgacgcgatc
       61 aatacctgcc gctgctctag gtgcttgccg ccgggcacca aggcaagctc atccgcttcc
      121 gagtcgaggg cttagatgcc acagcaagta ttggtcaagc ccggcgcgtc cggcatcccg
      181 aaaagaaatc tcgacagcct tcgctgcgat cggcgccaaa gtctgcgtct gcgacatcga
      241 gccagaagac ttccgagttc ccgggcagcg acgatgttaa agggtgcagc acgctttggt
      301 tatggatgat ccaacgatgt tcccggttgg tacgactcga agactaaatg gattccgacg
      361 gtcgcatccg acttggcccc ggactagctc agtgacggat cgcgcgcatc gcgccgtttc
      421 atctatatat tagccatgct ttgccagcac actgttgcag cccggccgcc tcgatcttct
      481 ctaagcttcg agtttttact tacaatcaag cctgatgctt agaagatagg gtgtggcagc
      541 tcgcggattt atttggctgg agaatagcaa gctgagatcg gttcgttgct gggaatgtat
      601 ttattgttgg agattgcctg gctgcatctg gttgccaatc actcctcggt gagcgtcgag
      661 gctccccgcg taggtgcaca agtaagaggt ttcactgact gaccggacgt tcgtagcgtg
      721 accggcgcac cccaagcgca taatgcagag ttgcgggcca atcttatttc ttggaaaaaa
      781 acctgcattc aggtaacggt ctaagctgaa ataggacctc aagtgcgagt tgtttgcccg
      841 gacaaacccg gcgacctcgt ggcgaagcag agatcaagat tgtccgatgg cggttaaatt
      901 cgcgattacc ctcactacaa aacccaaggc tcggctcatg tcacagataa atgtaaccga
      961 tcggaaccag caaaccttcc ccgcctcaaa accaacacaa ctgcacgtga gattattctg
     1021 caccatcctc ctgtccattt tattgtccgg tgcagcttac ccgcaggaat ctaacactct
     1081 cagtcgtccc cgtggatggg ctctgtcgca acgggctgca ccggtaccca gttttaacga
     1141 aggacgcttt accctttttc cggagctaag atcttcgggc gcccctctcc tgttagataa
     1201 aagatcttta ctggcacaaa cgatcaacgc gcctgcgcct gtcgcggcga gttccaatct
     1261 caccacgcca gtgccgcaat ggggaaccga aaagagctat ctgattccgg cgctggagat
     1321 cgtcggcttc gatgttttgc tcaatctgtt tgaccgtgtt tactacggct gctgcgatta
     1381 cgacgtcgat ttttcatcca tcaaacgcaa tttgcaccga ggctggaacg aggaccgcga
     1441 cgagttcacg gtcaaccaac ttgggcaccc ctaccaggga tcgatgtacc acggtttcgc
     1501 ccgcgcctcc gggttgaatt actggcaggg tctggcctac acctttgtag gaagcctgtt
     1561 ctgggagatt gccggcgaaa ctacaccgcc gtcgagaaac gaccagatca gcaccggaat
     1621 cggcggcagt ttcctaggcg aggcgttgtt ccgcatgtcc aacttctggc tcgagcaggg
     1681 gagaggatcc cctttctggc gcgaggtcgt cgccgcgcta atctctccgc ctgtgggctt
     1741 taaccgcctc gccttcgatc aacgtttcga tggcatattt cccagcaaag atcccgaata
     1801 ctacagccga atgcatttcg gactcgtctc ggcgactcaa gaccgccgcg gaggatcggg
     1861 agaaatcgac cggcacgaag gcatcgttga tgtggcgcta gactatggct tgcctggcaa
     1921 atcaggctac acctacgacc gtccgtttga ctacttcact ttccaggcag ccgcatccac
     1981 tgcgattggt ttcgagagcg tttccactta cgggctcttg ctgggaaaga gttacgacct
     2041 tgggaaggac tatcgcggca tatggggtct gtacggcagt tatagttaca tggcgccgca
     2101 aattttccgc ctggcaagca cgggcctctc gctcggcagc accgcccagt ggcgggtttt
     2161 cgattatctg gcggtgcagg gaacaggtct cctgggcatc ggctacgcca gtgtcagcga
     2221 cattgccggc atctccgatg aacgcgacaa tcactacggt gtagcgccgc aggcgcttct
     2281 atcgttgcga ttgatcctag gcgagcgggc gtccatcgac ctcaccgcgc gcgaattttt
     2341 tgtcgctgat gtatccggga gcggcgtccg tcacgacaat gtggtacgca cggatgcctc
     2401 gctcacatat cgcatttatc gtcagcatgc ggtgtcaata agatatcagc tgtcgcggcg
     2461 tgactttgat ttgcgcgata cgggaaacag aattcaggat agaggcacgg tcggaatttt
     2521 ctatacgctt ctcggacgcg atcggttcgg cgccggcgat tagcactgtg gagatcaggt
     2581 aactgtagtt gctgtctatc agctcggacc ggatcgatag ttctttgcgc tcgcactgac
     2641 atagccgctt cattctgggt tcaaacagat agccacgcgc gctgccgcgt ttggcgattt
     2701 tgttcttgac actattcggt tgcgctcggg cggcttatcg gtgcattccg cggacgattg
     2761 ggtgccgaca gctgcttgcc gttttaccac gctcccgatc gtaaaaccga gcagcgatct
     2821 cgtcgatcgc cctcttgcga agctgcggaa cctcaaatag aatccgttcg tttttcacgc
     2881 ctgtgaaagt cgtctcaagg aggatctatc atggcagaca aggccatcga ggttctgtta
     2941 aatgaaaaac ggaacttccc gccaccagca acatttagga aaacagctaa cttcaggaac
     3001 gcgagtgtct ttaattcggc gcgccgaaat ccgcaggcgt tttgggccaa agctgctaaa
     3061 gagcttcatt ggttcaagcc atggaagaaa gtgctggaat ggaaagcgcc atgggctaag
     3121 tggtttatcg gcggcaagct taacgcgtca tacaactgtc tggatcgcca tgtcgagaac
     3181 gggcggaaaa ataaagctgc aatcatttgg gagggcgagc cgggcgacga acgggttctg
     3241 acctatcgcg atgtctggcg tgaggtcaac aaattcgcca acgttctaaa aaaactcggc
     3301 gtgcgcaagg gcgaccgggt ttgtctctac atggggatgg ttcccgagct ggtcatcgcc
     3361 atgcacgcct gtaaccgcat cggcgcgccg cacagcgtgg tattcggggg cttcagcgct
     3421 gaagcattgc gcggtcgcat caatgatgcc caagcaaaga tcctgattac ggctgatggc
     3481 ggttatcgtc gcggcggcat tgtgccgctc aaagaaaacg ttgacgaggc gctgatcgac
     3541 acgccttcta tcgagcatgt cgtagtcttg gaacgaattg gacgcgagtc cggcgcacag
     3601 atgaaaagcg gccgcgacct atggtggcat gatctgatgg cggaggcgcc gctgtggtgc
     3661 gagcccgagc agatggacag cgaggacata ctgtacatcc tctacaccag cggcaccacc
     3721 ggaaaaccga agggcatcgt ccataccacc ggcggctatc ttaccggtgt gaacctggtc
     3781 aacaactggg tcttcgacct aaaagaggac gacgttttct ggtgcgccgc cgatatcggc
     3841 tgggttaccg gacacacgca tattgtctat gggcctatgg ccaatggcgc gacccaggtc
     3901 atgtacgagg gcactcccga ctggccggac aaggatcggc tgtggcgcct ggtggaaaag
     3961 tacggcgtga cgatttttca caccgcgccc accgcgattc gcacattcat gcgctgggga
     4021 acggaatttc cggcaaaaca caatctcaag accttgaggc tgcttggaac cgtcggggag
     4081 ccgatcaatc ccgaagcctg ggtttggtac cataaatata tcggtggtaa tcgctgcccg
     4141 gtcctcgata cctggtggca aactgaaacc ggccatcctt tgataacgcc gttgcccggt
     4201 atcacgaagt tgaagccagg ttctgcgacc ttgccgtttc cgggaatcga ggccgatgtc
     4261 gtcgacgaaa atggaaaatc agttcccaag ggcgggggcg gctatttggt tctgaccaaa
     4321 ccttggccgg cgatgatgcg caccatctat ggcgatccgg atcgttacgt gcagaattat
     4381 tggagccgct ttccgggaag atatttcgcc ggcgacggtt gcaagcgcga taaggacggt
     4441 tatttttggc tgctgggccg cgtcgacgac gtcatgaata tcgccggcca tcggatcagt
     4501 accatggaag tcgagagcgc tctggtggat caccaaaaag tcgctgaagc cgcggtcatc
     4561 ggcaaagagc acgacatcaa aggccaggcg atctgcgctt ttgtttcttt gaaagccggc
     4621 gtcgacgggg gcaattcgct tctggatgaa ctgaaaaatc acgtggtaaa aaagattgga
     4681 gccattgcgc ggcccgatga aattctcttt gccgcggaac tgccgaagac ccgcagcggt
     4741 aagatcatgc gccgtttgct gcgtgacatt gccgatgggc gcgcgttggg agacaccacg
     4801 acgctggcag acgccaatgt ggtggcaaaa ttgaaggagc aatatgccga ggagtgagcg
     4861 aatgagggtc gtccaatcga agctccatag cagaacaatt tcaattggaa cgagtaccat
     4921 tggcggtatg cggcgccatt ttactctatg gataatgctc tcgttgttgt ggtttcccag
     4981 tctaggcgat gcccagcagc gcgaaaaagt gcgcgtcgct ctgggttcga tttcggtaaa
     5041 ctcgagcgta ataccgatcg gcgctcagca tggaatgttt gcaaaatatg gcgtcgatct
     5101 ggagccgatc tattttggcg gcggcatgaa ctcgatcgcc gcgcttacat cgaattccgt
     5161 ccagctgctc gcggcgggtt ctactgcgac gatcggggca cgcgtcggtg gaatcgatat
     5221 caccatgttc gctgttcaat ccaacaagct ggattactcg gttatggtcg cccctgacgt
     5281 caaaactccc caggatctca agggcaagat cgtcaccggc actcgaaccg gcgcatccgc
     5341 ggatacggcg ctgcgcctgt acttgcaaag gcatggactc gtgcctgaca aagacgtcgt
     5401 tttcattgcg gtcgcggaaa gccagcaggg ccgtttcaat gccttggtcc gcagcgttgt
     5461 cgccggaacg gtgctgccgc cgccctacag cactgtcgcc aagcagcaag ggtttcgtga
     5521 attggcggat ttgcgcaaaa ccgatatcga gtattcggga acttcgattg ccgggatcgg
     5581 ttcgtacgtc aaggctcatc cggccgcgat cgaaggattt ctaaaaggtt atatcgagtc
     5641 gttaaatttc ttccgtacgg aaaaagagaa atcgatcgcc gggatcatga aatatctgcg
     5701 tatcagcgat cgatcacgcg ccgaggaagg atatgagtac tatgtagata tgatgccggt
     5761 gatgccgtat gccagcaatg ccggcgtcaa ggcggtcctg caattcctcg ggacgcgcaa
     5821 tcccaaggcg cttaccgcga accctgagga cttctacgat catagttttc tcaagaagat
     5881 agaagaaagc ggctttagca aacagtttgt ggcgcggcgc taaatggacg cgaactgcag
     5941 cggcaaattc ggaggttgcg aagcatggag aaaatgtcgg caggaagaat attcctaatc
     6001 gcattcctag cgattgcgct tgccgggatc ggttcgctcg aagcgcaaga taagccatta
     6061 aagaaaatca gctggggagt cacttcgttg tccgcatcca attggattcc gtggatcgcc
     6121 aaggatgcca agatttacga gaagcacgga ctggatgtcg aattggttct agtcaaaggt
     6181 tcaggccaga cctcgactgc gattctcggt ggcagtctct ttgccgcgcc ggttgccgtt
     6241 ccgcaagtga tgctggccga tctcgccggc gctgatctgg tgaacatcgc tcatacggtc
     6301 ccgggagttc aaagcaaact gctggtgaag caggaaatca aacggcccga ggatatcaag
     6361 ggaaaaagga ttgcaacctc gagcataggc tctctcggag atttcctttt taagtacatc
     6421 atccggaaga acggtatgga tccgatgcgc gacgtcatct ggctgtcgat cggcacgccg
     6481 caggagcggc tccaagcgct gtcatcgggc cgcgcagatg cggcggacct gtcgtatccg
     6541 attgatgccc aagcgctgcg gctgggctat cacgtcttgt gggacgccag aaaagaggtg
     6601 gtctacccgt cgatgtcagt cgtgacccgg cggaaaactg tgcaagacga ccgcgatacc
     6661 gtgatgcgca tgctcaaatc ccatgtcgag gcgattgcct acttcaaagc acacaaagat
     6721 ttcagcatga aagttctagg caaatacctg cgcaccaatg atcaggaact gttggaaggc
     6781 tcgtacgaaa tattcaggca ggatttcatc tcggtaccct atccgatcat gaacgggctc
     6841 gaagccacct acgactatgt cgcgcaaacc aggccggaga ttcgcagtcg caaaccagag
     6901 gagttcatgg acccgagcct catggccgag ctggacaaga gcggtttcat caagaagctc
     6961 tatgcgcaga agtgatcgtc ggcctttttg aattcaacta aagctctcca aaatttctgt
     7021 ccttcgatga gggcaccggt caacggttca aaattcaacc gctggactct acggagccga
     7081 accggatggt gatggccaag ttaaaagaca ttctcaagta aaaccagtct cgctctcggt
     7141 cgcaggaggt atgaacatgc gtctctcaag caagttattg gcgtcgatat ttcttctggg
     7201 gattgcgttc gccgggctca aaatgccgag cgcgcgcgcc gcatcggttg aagagattgc
     7261 cctgctcaat aagcccgacc ggcagaagat ccttatggaa ggagcgaaga aagaaggaaa
     7321 gatttcctgg tatacatcgc tgatcgtcga ccaggttgtg cgtccggtta cagcagcatt
     7381 cgagaaagag tatccgttta ttcaggtcga gttctttcgc gccaattccg agcggatcgt
     7441 gcaaagaatg tttagcgagt atcaggccaa gcgctacgac gtcgatttgc tcgatggaac
     7501 ggtgacggca gcgatgatac gacgcgccaa ctttttgcag cgctttcatt caccttatct
     7561 ggccgagcac tcggcggaaa caagagaccc gcaaggcaat tgggcatcga ccaacgttta
     7621 cttctttgcg ctcggctaca acacgcgtat ggtcaagccc aacgaggtgc cgaaaaccta
     7681 tgaagatctg ctcaacccgc gttggaaagg ccagatgatg tggtcgacta gccgcggctc
     7741 cggcgcgccg atgtttatcg gcaacgtcct gcagtccatg ggagaaagcg gcggcaaggc
     7801 gtacctgcaa aaattaaaag cacagaatat agccaagagc acggcaagca atcgacagtt
     7861 attggatctg gtgatcgccg gcgaatatcc gatcgccctg catattttta atcatcatgc
     7921 ctacatcagc aaaaccgccg gggcgccagt ggactggcag gcgctggagc ctgtcaccgc
     7981 aactctcaat accatagctc tcgcctcacg agcgccgcac cctcacgcgt ccatgctgtt
     8041 cctggatttc atcctgtccg aacgaggaca aaaggtgttt caacaggtta attatttgcc
     8101 gtctcacccc aaagttccag caaagcaacc ggacttaaag ccgggcggcg gccgtttcaa
     8161 caaagcgctc tacatcacgc cggaagctca gtttgagaaa ggcaatgact gggtggatta
     8221 ctttaacaaa cagttcatga attaacaggt tgctgaaaag attcataggt tcgttgcgct
     8281 cgcccgactc gcttcaacgt actgaagcgt acgcctcagc tcatcgagct tcacgcggct
     8341 tccctctgat atcttttgag cagcctggat acagagtttt tactaaactg ttagtacgcc
     8401 gcggctcgtc gctggtcggc aaactatgat gaatctgttc gcctcttgtt tagctcgtcc
     8461 aggttccctc cttaactaat acacattgtc aaacattacc ctggaactgt gcaatctgtg
     8521 tcagatttca tcttgacttt cgattgtggc ggagactagc ttcgaggcga ggtattttgt
     8581 cgcccatgaa ccggcaacaa tcaaccgaga ttaacctgtc tgcgcaatcg gcaaccgtgc
     8641 cggcgccgcg gcgggttttt ccgttgagca tggggtcccg ctggggcatc gagtcttggg
     8701 tgatgatcgt cgtcacgttg atcaccgcct atctggtgct gccgccgctt tactcggtca
     8761 tccagaccag cctgtttaca accactttta ccggcgaact ggatcagttc actttgcgtt
     8821 attatgaaga gctgttgcgc gagctccggg ttctggggcc gtttctaaac acgctttatt
     8881 tttctattgg cagcgcgtta ttggcgaccc tgatcggcgg catggttgcc tgggtggtta
     8941 cccgcaccga tacgccgctg cgcggtctcg gctacttcac ggcgttcgca tccttcggga
     9001 cgccgttcat cttgtacacg attggctgga tcttgctgct cggcaaagcc ggtccggtca
     9061 atcactggtt gaagattctg ttcaatcaga acacgccggt gatcaacgtt tactcgatgg
     9121 cagggatgat cttcattgaa gcattgcttt ggtcgccttt cgtgttcctc atgctggcgg
     9181 cttcttttcg ctcgatggac ccatcgctgg aggaggcctc ggctgcctgt ggcgccaggg
     9241 tttggcagac tttgcggcgc atctcgctgc gcttgatgct gccggcgttt ttttcagtgc
     9301 tcctgctgat ctttattcgc gccttcgaat cgtttgaaat tccggctttg gtcggacttc
     9361 ccggtgatat tcgtgtgctc acaacttcga tctatcagga cgcgcagcga ctgccgccgc
     9421 agtacggtaa tgccggagcc ttcgccgtgc tgctcatggt ggtggtggcg gcgaccttgt
     9481 atgtttattt tcgcgccacc cgggacggtg atcgattcca caccgtaacg ggaaagggct
     9541 accgaccggc gctaatccgg ctcggccgct ggcgctatgt aagcgccgcc ggattgctgt
     9601 tctattcgtt cgtcctgctg gtcctgccat ttctgattat tctctgggct tctctgctgc
     9661 cgttctatat ccagccatcg cttgaggcga tatcaaaatt taccgtgaaa aattatcttc
     9721 tggcgattca ctttccgaaa atcatcgcct cgatcaagaa cagcattttg ttaggcttgg
     9781 tcagcgcgac tatggtgatg gtgctgacaa cattggcatc gtggatactg gttcggagca
     9841 agctgcgcgg gcgttggctg ttggacttgt taacgacact gccgctactt tttcccggga
     9901 tcgtcatggg attggcgatc atgcggtttt atttgaatgt gccgatacct atctatggca
     9961 ctctatggat cctattgctc gcgttcgtta ccaggtacat cccttacggc atccgctata
    10021 cgcattcagg tttgctgcag ttgcacaagg aactggaaga agcctcgtat gtttccggcg
    10081 cctcatgggt taactgcatg cgccggatca ccttgccgct gattatgccg tcgtttctcg
    10141 gcggatgggt attcgtcttc ttgctttcag ccaaagaact ttccatgtcg attcttttgg
    10201 taagcccgca gacgccggtg gtttcagtcg ctatttacga gctctgggaa aatggtcagg
    10261 taggcgagct ggcggcattc agtgtgatct ggacggcgat actggtgagc gttgcggtta
    10321 gctattatct gtttgctcgc cggttcggag ttcagcagga ttgaggcgga actcgcagcc
    10381 agccggcaat atcaggaacg atggcgctgc gtgcgagact ccatgcgacg ctccggacac
    10441 ctttattcaa cagtccttga tgccagagat ctagcaggct gctgaaaaag acccatatgc
    10501 ttcgtcgatt tctcgcgcgc ctcgcaactg aacctttttg agcagcctga aagatgcttt
    10561 tcagcaccct gctattggct cttttctgcg cgagtgggta ggcggttctc cgctggagat
    10621 ggttcaggcg ccctgctggg atcctcaacg ccgccctgtt gcttttgcgg ctgttggtac
    10681 tgcttgaaaa ggggcgaggt gcgcagcgga gcgtcggcgg gtattcgatc ctggcaagcc
    10741 tttccgaaga acatcagcat tcccaccgag acaacgatgg aggctagggt aaagaacggc
    10801 atcagaacgt aaccccagcg tcggcgacgt tgctcggcct taaaatcttc aagagttctg
    10861 ggcgggccgt aatccatatt cacgtcaagg cggtctgatc cgatcagaga gcgtaggaaa
    10921 tatattatgc ggtcaccgcc atgtccatcg cggtgatcgg cagcgctcgt aattgggccg
    10981 ggtcagacga taacagttga ggcattcggc gttatttcct ttagacaaca gatctgcggt
    11041 tatcgacaga tgagttgtcg agataaagct tttctccaga cctcgtttgt tgctatattg
    11101 gcgttatgga aatttctagg gatcgacccg taccatcaca tgatgaggac ccgacgccgc
    11161 aaagctggtc ggagtattgt ccgatttgcg gttcgaatat gttcaatcaa aagtgccgtt
    11221 acctttgttc gaatccccat tgtcgctttt tcatgagttg cagcgaattc gatcgttgag
    11281 cgagcttgcc ccgccaggcg cgtttaagct ggaagattaa tcatgtcgaa aaccgccggc
    11341 atcatcatca tcggcaatga agttctctcg ggcaagaccc aggatacaaa ttctcacttt
    11401 ttgtgtgttg agctccgacg ccttggcgtg gaggttcaaa agatttccac gattcaggac
    11461 gagatcgatc tgattgggca ggaagcggca tatttctcga agcgattcga tttcgtcttt
    11521 acgaccggcg gcgtcggtcc tacgcatgac gacgttacta tcgaaggcat agccaaaggg
    11581 ttttctgttc cagtcgtccg ccatccggaa attgagcacc gcatgcgcca gcgcctgggc
    11641 ggcgatgtta accaagcgcg cttgaggatg gccaatgttc ctgaaggtgc cgtgctattg
    11701 gcgaccgatg cgctatttgc accggtcgtc aatattcaca atgtttacat ttttccgggt
    11761 ataccacgca ttcttcagga acggtttcat gcgatcaagg aacgatttcg cgatgcgccc
    11821 tatttcctca agaatgtttt tgtcagatac ggcgaagggg tcatcgcttc cgcactgaac
    11881 aatttgctgg cggagttccc ccatctgctt ctcggttcct acccggttct ggatctgccg
    11941 gactacaaag taaaagtcac gttggagt
//
LOCUS       DASUVS010000343         5547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_143759, whole genome shotgun sequence.
ACCESSION   DASUVS010000343 DASUVS010000000
VERSION     DASUVS010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143759"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VF182_18280"
     CDS             <1..511
                     /locus_tag="VF182_18280"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX6439086.1"
                     /translation="WFMTNRHFLTDYRRIHDFARFPELRWLNRFDVIVPIILAVTLYF
                     VGRLLHYAAPSLGVTGWQLVVWGFVVSTLMLFHATASINSLAHLIGRRRYDTGDESRN
                     NFILALITLGEGWHNNHHKFRSCTRQGFYWWEIDLTYYALKFFAAIGLIWDLRPVPGK
                     AYDHNQQLS"
     gene            791..4990
                     /locus_tag="VF182_18285"
     CDS             791..4990
                     /locus_tag="VF182_18285"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX6439087.1"
                     /translation="MSRSEDPGLEVDSADAEQNARVNDPDGHSPFPIVGIGASAGGLE
                     AFQQLLGQLPADINMAFLLVQHLDATHESHLTHILSRATRLPVSEAIHGRAVRPGEIY
                     VIAPNTCLAVTDGLLHVTPRDQLPGPHLPIDHLFRSLALEQQGRAIGVVLSGTGADGT
                     MGLCEIKGAGGITFAQSEASATHAGMPHSAIESGSVDFVLNPDAIARQLAEIASHPYL
                     DPLQRVESVAASGEHYKQIVGAVRAVTKVNFSHYRESTIQRRILRRMALQNQQSMADY
                     ARRLEQDATEVEALYRDLLINVTSFFRDPEVFDYLKDKVFPQVIKGKGDAPVRIWVPG
                     CSTGQEAYSLAIILWEFFDDRPVRPPIQIFASDMADTGLLEKARAGLFPASIESEVEP
                     GRLRRFFKKEDHLYRIDKSIRDACVFAHHNVMADPPFSHVDLISCRNLLIYLEASAQK
                     RVLSTFHYALNLPGFLVLGTAESVSGKADYFARLDREHKVYKKNPIVSHPPMFMAEEA
                     YKDKKNTTPERSQKGLSAEYQLRADRLILAEYAPPGVLINDNFDVLQFRGDTRPFLSL
                     PPGQPTVNLLRLVRQGLFLELRSALTEAKESNRPVRRERVQLHDEHELREITLRVVPF
                     QSGNDERCFLVLFEPALSVATAVTPSTPAGGWVTRLKRLLRGGEHEGPEASRRSPAAA
                     RFSSVNSDVEVERLQRELTSVKEYLQATIEQQDTLSEELRSANEETQSSNEELNSTNE
                     ELQTAKEELQSSNQELTTLNEQLRYRNLELSEIGDDLTNLITSTTIPVIMVGNDLRLR
                     RITAPARRVMNLQADYIGRPLRELATNFDLPELEGLITEVIDQVQPREVEVRDRHGVW
                     YLLRIHPYRTTENKIDGAVLVLLDINQIKVFQQVQQEIEARLHLALEGGSLGTWYSDL
                     TSGGWSWDDNTNSILGLPAYADRNEVTFFARVHPEDQEKFGVLRNAAEIRDRYMDEIR
                     IVQPNGAVRWILLQAKVFYSPSGAASRLSGICMDITERKELAEKLRLNMEELRLADQR
                     KSEFIAILSHELRNPLAPIINAVQVLRAKDVSETQRDWSREIIDRQVQQMIRIMDDLL
                     DVGRITHEKLSLRREQILLTSVIDNAIETSKPLIDERRQQLTVDLPAERIVLNGDYRR
                     LSQVFSNLLNNAAKYTPNEGRIVLAAKRQDNDAVISIKDTGIGISAELLPDIFDIFVQ
                     AKSSHESERGGLGLGLTLVRSLVELHGGNVEVKSEGLGRGSEFVVRIPIGEGDSVIED
                     QDGAARPNAQTVVEKGRRILIVDDLAIQAKSLAILLQLRGHDVRIAHNGPDALQILQE
                     FPTEVALIDIGLPGMDGYSLAGRIREQPQFSKTLLIAQTGWAMEDDRLRSREAGFDYH
                     LAKPIDHGRLHEILTESHTDT"
BASE COUNT         1312 a         1501 c         1532 g         1202 t
ORIGIN      
        1 atggttcatg accaatcggc atttcttaac cgactatcgc cgcattcacg actttgcccg
       61 cttccccgaa ttgcgatggt tgaatcgttt tgacgtgatc gtaccgatca ttctcgcagt
      121 gacgttgtac ttcgtcggtc gtttgcttca ttacgccgct ccatccctgg gagttaccgg
      181 gtggcagtta gtcgtctggg gcttcgtggt tagtacgcta atgctgtttc atgccacggc
      241 gagcatcaat tcgctggcgc atctaatcgg ccgccgccgc tatgataccg gtgatgagag
      301 ccgcaataac tttatcctcg ctttgattac cctgggcgaa ggttggcaca acaaccatca
      361 caagtttagg agttgtaccc gacaaggatt ttattggtgg gaaatcgatt tgacgtacta
      421 tgctcttaag tttttcgccg ccattggact catctgggac ctcagacccg tccccggcaa
      481 agcgtatgac cacaaccagc agctgtcgtg agaaaaacca agcctagaaa cggttgccag
      541 gcgccggcaa atgactagga atcgcgtcga ggaatcggaa tcagagcacc ttcatgacct
      601 cgttatggca gattccctct catcgcaagt ctaaacagcg agtatcaaac ggcgggggaa
      661 ccgccaaaca gcaagtggag acttgcgtta attcgggatt gggcaggact cgtaggtgaa
      721 caccgacgca ttatttcccg ttgtacaaaa taccgctgag tggtagagcc aactaggatc
      781 agagttattc atgagccgca gcgaagaccc gggactggaa gtagattctg ctgacgccga
      841 gcagaatgca agggtcaacg acccggacgg gcacagcccg tttccgatcg tcggcatcgg
      901 cgcgtcggcc ggtgggcttg aagcgtttca gcagttgttg ggtcagctcc ccgccgacat
      961 caacatggct ttcttactcg tccagcacct cgatgccacc cacgagagcc acctcactca
     1021 tattctttcc cgtgccacgc ggctgccggt atcagaggca attcacggac gtgccgtgcg
     1081 ccccggggaa atctacgtca tcgcccccaa tacatgtttg gcggttactg acggcctgct
     1141 gcatgtaaca ccgcgcgatc aattgccggg accgcatttg cccattgatc acctttttcg
     1201 gtccctcgct ctcgagcagc agggacgtgc cattggagtg gtgctctcag ggacgggagc
     1261 cgacggcacc atgggactgt gcgaaattaa aggcgccggc ggtattactt ttgcccagag
     1321 cgaagcctcg gccactcatg ccggcatgcc acacagcgcg atcgagagtg gcagcgtgga
     1381 tttcgtgttg aaccccgacg cgattgcccg gcagctggct gaaattgcca gccatccgta
     1441 tctcgatccg ttgcagcgcg ttgaatctgt ggccgcatcg ggcgagcact acaaacaaat
     1501 cgttggcgcg gtccgggcgg ttacaaaggt caatttcagc cactatcgtg aaagtacgat
     1561 tcagcgacgg atcctgcgcc gcatggcgct tcagaatcag caatcgatgg ccgactatgc
     1621 gcgccgcttg gaacaagatg ccaccgaggt cgaagcccta taccgggacc tcttgatcaa
     1681 cgtcacgagt ttttttcgcg atcccgaagt tttcgactac ttgaaagata aggtctttcc
     1741 gcaggtcatc aagggcaagg gagacgcgcc ggtgcgtatc tgggtgccgg gttgttcgac
     1801 cgggcaggag gcctactcgc tggcgattat cttgtgggaa ttttttgatg atcggccagt
     1861 gcgtccgccg atccagattt tcgccagtga tatggcggat accggcctac ttgagaaggc
     1921 tcgcgcgggg ctcttccccg ccagcataga gtctgaggtg gagcccgggc gactacggcg
     1981 tttttttaag aaagaagatc acctttatcg gattgacaaa tcgatacgcg atgcctgcgt
     2041 ctttgcgcat cacaacgtca tggctgatcc gccgttttcc cacgtcgatc tcatcagctg
     2101 ccgtaattta cttatctacc ttgaagcttc tgcacaaaag cgggtgctct cgacgttcca
     2161 ttatgcgctc aacttgccgg gctttctcgt gcttggaaca gccgaaagcg tgagcggtaa
     2221 ggccgattac tttgcgcggc tcgatcgcga acacaaggta tataagaaaa acccgatcgt
     2281 aagccaccca cctatgttca tggcggaaga ggcttataag gataaaaaaa ataccactcc
     2341 tgaaagaagc cagaaaggcc tctcggctga ataccagctg cgggccgacc gtttaatcct
     2401 ggctgagtat gcgccccccg gtgtgttgat caacgataac ttcgacgtcc tccagttccg
     2461 tggagacacc cgaccttttc tgtcgctacc gcccgggcaa ccgacggtca atctacttag
     2521 attagtccgc cagggtttgt tcctggagct gcggagcgcg ctcaccgagg caaaagagag
     2581 caatcggccg gtacgccgcg agagagtgca attacacgat gagcatgagc ttcgggagat
     2641 aaccctccgg gtcgttccct tccagtccgg caatgatgag cgctgttttt tagttctctt
     2701 tgaaccggct ttatctgtcg cgaccgcggt gacgccgtcg acacctgcag gcggatgggt
     2761 cacgcgcctt aagcggttgc tgcgcggagg ggaacatgag ggtcccgagg cttcgcgccg
     2821 ctcacctgcc gctgcgaggt tttccagcgt aaattctgat gttgaagtcg agcgactcca
     2881 gcgtgaactg acttccgtga aagaatacct acaggcgacc atcgagcagc aggacaccct
     2941 gagtgaggag ctccgctcag ccaacgaaga gactcaatct tccaacgaag aactaaatag
     3001 cactaacgag gaactgcaga cggccaagga agagctgcag tcctcaaacc aagagctcac
     3061 gaccctgaac gaacaactgc gctaccgcaa cctggagctg agcgaaatcg gcgatgattt
     3121 aaccaatctg ataaccagca ccacgatccc ggtcattatg gtcggcaatg acctacgtct
     3181 gcggcgtatc acggcaccgg cgcggcgcgt catgaacctt caagccgatt acatcggccg
     3241 gcctctaaga gagcttgcga ccaacttcga tctgcccgag ctggagggcc tcatcacaga
     3301 agtgatcgat caagtgcaac cgcgtgaagt agaggtgcgc gatcgacacg gcgtctggta
     3361 tcttctgcgc atccaccctt accgtactac cgaaaacaaa attgacggcg ccgtgctggt
     3421 attactcgac atcaatcaga tcaaagtctt ccagcaagtg cagcaagaga tcgaggcgcg
     3481 gctacacctg gcgctcgaag gaggctcgct cggtacctgg tatagcgatc tcactagcgg
     3541 gggatggtcc tgggacgaca ataccaacag catcctcgga ctgcctgcat atgcggaccg
     3601 caacgaggtc accttctttg cccgggttca tcccgaggac caggagaagt ttggggtgct
     3661 gcgcaacgct gcggaaatcc gtgatcgtta catggatgag atcaggatcg tacagcctaa
     3721 cggcgcggta cggtggatat tgctgcaggc caaggttttc tacagcccat caggggcggc
     3781 atcgcggtta agcggaatct gcatggacat caccgagcgc aaagaactgg ctgagaaact
     3841 aagactgaac atggaagagc tccggctagc cgatcagagg aaaagtgagt tcattgccat
     3901 tctatcgcac gagctgcgca atcctctggc gcccatcatc aatgcagttc aggtgctccg
     3961 cgctaaagat gtgtctgaga cccagcgtga ctggagtagg gaaattatcg atcggcaggt
     4021 tcaacagatg atccgtatca tggatgattt gctcgatgtc ggtcggatta cgcatgaaaa
     4081 gttgagtctt cgcagagagc agattctcct gacaagcgtc atcgacaatg ccatcgagac
     4141 cagcaagcct ttgatcgatg aacggcgcca gcaactgaca gttgatttac cggctgagcg
     4201 gattgtactg aacggcgatt accgccggct gtcacaagtc ttttcaaacc tgcttaacaa
     4261 cgcggcaaag tacacgccca atgaagggcg catagtgctc gccgcgaagc gacaggataa
     4321 cgacgcggta atatcgatca aggataccgg catcggtatt agcgccgagt tgttgcccga
     4381 tatctttgac atctttgtac aggcaaaatc ttctcatgag agcgaacgag gcggtctcgg
     4441 tctcggactg acattggtgc gcagtcttgt tgagctgcac ggcggcaatg tcgaggtcaa
     4501 aagcgagggc ttaggtcgtg gaagcgagtt tgttgtccga ataccaattg gcgagggcga
     4561 ttccgtgatc gaagatcagg atggtgccgc acgaccgaat gcacaaactg tcgtcgaaaa
     4621 aggccgtcgt attctgatcg tcgatgacct cgcaatacag gcgaagagtt tagccatcct
     4681 cctccaactg agggggcatg atgtgcgtat cgcacacaat ggcccagatg cgttgcagat
     4741 cctgcaagag ttcccgacgg aggtagcgct gatagacatc ggtttacctg gtatggacgg
     4801 ctacagcctt gccggccgaa tccgagagca gccgcaattc agcaaaacgc tattgatcgc
     4861 gcagactggc tgggcaatgg aagatgaccg cctgcgctct cgagaggctg gattcgatta
     4921 tcacttggcc aagccgatcg atcacggccg cctccatgaa atcctgaccg aatcccacac
     4981 cgacacttag ccgagcttgc tagaaggtgg caggagtacc tgccagatgg agcgtcggtt
     5041 agccgactgc cgtattgaag ccctgctgtg tttgctcgac acgacaccta agatcgtaga
     5101 gttcggttcc gttaagcggt ttatcttcct gcttggcgtt gcatccgtgt catccaaccc
     5161 aaacgcgacg ttgttatccg taatgctgca agaagcgact ggaatctgat cgaatccgaa
     5221 agagcacggt gaatgcttct gatgcgtctc gtggtcatca gcatcgatga tatggccaag
     5281 acgacggctt aaatgaacaa agagcatcaa gaaatgccat agcgtcctaa gagagacagt
     5341 ccagttgatc ccagaaaacc tgtttgcctc gtcctagcgt agagaatttt attgacaagc
     5401 agtgacaaaa atttggacac ttagggcgga ggacatatga ccaaagtcat gcaatcggtc
     5461 agaggcgtag tgcaagggac tcttcatcgg cgaaaaccgc atctcatgat ttccgcgcgg
     5521 gtaaaagata gcgacggcag tttaaac
//
LOCUS       DASUVS010000344         4746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1676340, whole genome shotgun sequence.
ACCESSION   DASUVS010000344 DASUVS010000000
VERSION     DASUVS010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676340"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2990..3364
                     /locus_tag="VF182_18290"
     CDS             2990..3364
                     /locus_tag="VF182_18290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439088.1"
                     /translation="MEKRFQRNDPDVFDLAWLDDEELKALQRAMLSNHTNEICTYWLR
                     SLYEQSAIRTRKLRVIPGTEARDPDFKQYSISDLDSAQLIFRRMENQFNAIGKSMGGL
                     FCELVVKCCREAIERKQESSVP"
     gene            complement(4139..4214)
                     /locus_tag="VF182_18295"
     tRNA            complement(4139..4214)
                     /locus_tag="VF182_18295"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4179..4181),aa:Ala,seq:cgc)
     gene            complement(4218..>4746)
                     /locus_tag="VF182_18300"
     CDS             complement(4218..>4746)
                     /locus_tag="VF182_18300"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX6439089.1"
                     /translation="VLCARDLVGDEPFAVMLADDLIDAETPGIRQLVEIFTETEESVV
                     ALMKVPREEVHQYGVIKGKEVKPRLYAVEATVEKPTAKEAPSQLAIIGRYVLRPEIFS
                     ILQTLPPGRGGEIQLTDGLARLVQERRIFGCEFAGDRYDIGDKFGFVRATVAYALKRK
                     DLRDKLIEYLKSLND"
BASE COUNT         1184 a         1198 c         1194 g         1170 t
ORIGIN      
        1 taacagcaaa cgactgcatt ctgccttggg atatctaccc ccaaaggaat acgagatggc
       61 aattcaaaca accgcaaaaa ctgccggtcg cctctccctc aaatctcaat gaaatgtctc
      121 cagtcggagg ggcgcagtcc aagggttatc ttccacgatc aggattctcg ttcgcctacc
      181 tttgtcgaat catcatgaac caactaacaa agagaatggc gcgccgggaa cgaaaagaat
      241 ctttggtgta gttacgagtt gcgcttcttc gccccggcca ctagccgact gggcaagcgt
      301 tggaccgtca ctgttgatcg cagcagcaat aactctggag cacttcggaa cgtgtgcggg
      361 agctctgtat cggaatgccg tgcggcaggt ttgtctacta gtcaaaacgc cccttcagtg
      421 atcggttaag tttgccgtgg ttcagtaaaa acaaaggaca acaaaataga tagacctcaa
      481 caacacaccg gacaattcgt gtgctccctg atccggatga aatcttcatc aggttcgacg
      541 agggaaatca aaacgcataa ggcacttcgg acagtttaat cggaaaaagg atttctcctc
      601 gtcgctatcc ttgctcttct atcagtgctc aagcttttgc gggcacaccc gttttcggtc
      661 ctattaaagg gacatccacc gatagcagca tcatgaggag ctgatcaagt gcttgccatt
      721 gcttgtaggc cagtagatgc gctttgacat gtaaggtcta tccacggatt gattccgtac
      781 tcaatcccat aatagacaag gcggacacat caataatcgc acaagttctc ccagctcttc
      841 agggctattg gcgacacgaa acttagaagc gttggccgat gatagaactt caattcacgg
      901 cgcgccggaa caatgatggg ttaaatgccg gacttctgcc gtagactcgt attctcggtg
      961 tgattctccc cgagagaaac gatctcttat ccgacatgga tgcgcaccct acgctgctta
     1021 tagagatgaa cccacatgtc gtatatctgt attaatgtaa gcgtttccat cttctgcgtc
     1081 ccggctaatc ctgtataaga cacgctcagt gtgtcagcag cctttgagat tttatgtatc
     1141 cggcctttct tgtgatcaac ataccatcct accgtccgta ggacctgcgg caagctgtgc
     1201 gatagtgggc tgcgttccca atccgatcgt agagcctgcc cgtctcgttc gagcttatca
     1261 atgtccgctt gcataaattg cagccgcagc acgcgagaag agccggggac tcccgcgtcg
     1321 ggaattgcgg acggcgtgga tgttttcgag gtattgcaga tgtcgaggaa ggctttcttg
     1381 aacgctttta gctttgtaac gggcgacttc tcagatgctt ttcgggtcgg aattcctcgg
     1441 actgaaaaag tatcatcagc tacttccaaa tcgaacgctt ccaacgatag cgactcaagg
     1501 ccctgcccta tagcgcgaag aatgtgctcg taacaaacca cagggtgttc catgagagtg
     1561 ccagatttgc atggtctgtg cccgcttaag gcgctcagtt attaccgagt ttctctaaat
     1621 ttcctacggg tgatggcacg catacctgat aatacttcgg tctgtgattg gagtagacaa
     1681 aatcgccgct gatcatcggg tgcctgggac cagtaactga actgtcaaag gatgagtctt
     1741 agacaagccg gaaccgtgcg gtccgcaacg ggtataatca gccgcgattt cattctccgg
     1801 cttgtttact agccgctctt gcagcagatc tagctgtttt tgcttttggc ttcggaggga
     1861 gtacgacgta aacggcgccc ccgcaaatcc cgcaggttag catagctcgt ggatgaactg
     1921 atacgaatta tgggtcgggt gacgatagcg aaattataca ttgcgcttcg tttttatagg
     1981 atctccagga ctgaatgctg cccccttctg ttgagctttc ctcttttcga ctatcattga
     2041 tcggctgtaa gctgcaccaa caatagatct ggacgttggc accgcggtga ttattcgaat
     2101 cgtggagcac agatcgtttc ggctattctt gcagggtccg gatatccagc acattggtgt
     2161 ttgctcatta acgttgctgt gagatgcgtt tacgcttcgg ctggatccgg gtgtactgcc
     2221 aagcctaaca tcaaccggct tggtcgatga tgaatttctt ggcttgcact tcactctgaa
     2281 acacatcgtc gggtcttaga acgaccgtat gaactttgag cttgtcacta actcaatgtg
     2341 aacagacaaa taaacccgat caatatccgt ttcgtcggct tagcaggcaa agctgccgcc
     2401 actgcggcta gacagaaccg cgtatgcatc gccaactctt ccgacgtata gcttgcctga
     2461 tgaaggactg acgggcacac cccttgctca gcatgcgcca gcagctgccc gaaggaacgg
     2521 cgggtgaatg aaaccgttga tggtcgaaaa ttccgtacag ctctcaagac gtgacggcat
     2581 cacgctaatg ttagctttct cttcatacgg tttaccggct acatcgccgg agcctaagtt
     2641 acccgtatct tagagaagac gaaggtcatg taccagtcca tcttcacacc gccacctgaa
     2701 aaccgtagcc gggtatagcg cagaaggatg acttcaatga ggtggctaga aattccggct
     2761 catagagtac ttgaagtgga gtgtaacgaa ctcgaagacg agttcgatat gtatgatgac
     2821 cagggcaacc cttgcggtgg gagcagcgta tatcttttgt tccgtagaaa agacggcaag
     2881 agattcaagt tcgctcagtt cgggaacgtc tacggggcta tcaagcggaa ttgcgcttat
     2941 ctgcaggagg tctgaacagt cagtcatcag caacaggcag gtttagatca tggagaaacg
     3001 atttcaaaga aatgatcctg acgtcttcga tctcgcttgg ttggacgatg aagagctcaa
     3061 agcattacaa cgggcgatgc tttcaaatca tacaaatgag atctgcacgt attggctgcg
     3121 gtcgttatat gagcagagcg ccattcgcac tcggaaactg cgggttatcc ccggcaccga
     3181 ggccagagac ccagacttca agcagtattc gatcagcgat cttgattcag cgcagttaat
     3241 cttccgccgt atggaaaatc agttcaacgc catcggcaag tcaatgggcg ggctcttttg
     3301 tgaactggtg gttaaatgtt gtagggaggc gatagagcgc aagcaagagt cttcagtccc
     3361 ctaatggctg aggcatgatt acctcggacc acgagatcgg cgtctttgtc atctctccaa
     3421 ccgcagattg ccgtgcctca acttccgaat cagggatctg cattctgtag cttcgatatg
     3481 cgcaactaaa cttggttcaa gccagacgcc gttctttatc gcccttgggt tagcgccaat
     3541 tcaaccattc actgctgccg cactatctgc agaagggccg tgcgcctgtg atgcacccct
     3601 catcaataaa gagaatctcg aaagacccgt tctggtcaga ttgaggatca gattttaaat
     3661 gaacagaatt tgcccaaata cattgattgt ctgcaggctg gctcggcaaa ggctgaaccg
     3721 ggtgcagagg agaaagcggt ttatttttct ctagcgcatc gattcccaga ttagacggtg
     3781 gcgggagacg ctcgagcgcg cgcttgtatc tctggaagac tctgctgatc gcatcaaaga
     3841 gcttagccaa gatcgggagg cgcttttgag acgcaaactg gaattataga aaatgtcgtt
     3901 cggcggtgcc tattttgccg atgagaactg agatcgtggc tcaatatgtc cgggaaatgc
     3961 aggcaggcat atacgaagaa atatcggcta taaaaaggaa atcgcgacag attttgtgaa
     4021 agaggtagaa tcgacagtaa gaccgtcacc ctgaagtaca ggcttctagt tacagtaaag
     4081 aactcctcct gcctgggcct aaaaaccggc aaagaggagt tctttacact gtataacttg
     4141 gtggagccga tgggggtcga acccacgacc tctagagtgc gattctagcg ctctcccaac
     4201 tgagctacgg ccccagacta atcgttcaac gatttcagat actcgattaa cttatccctt
     4261 aggtcttttc gtttcaatgc ataggctaca gtcgctcgaa caaagccgaa cttatcgccg
     4321 atatcgtatc gatccccggc gaactcgcaa ccgaagattc ttcgttcctg aaccaggcgg
     4381 gccaatccgt cggtaagttg aatttcgccg cctcgtcccg gtggaagagt ctgcaaaata
     4441 gaaaaaatct ctgggcgcag cacgtaacgg ccgatgatcg ctaattggga cggcgcctct
     4501 tttgcggttg gtttttccac agtagcttcc actgcgtaaa ggcgaggctt cacctcttta
     4561 cctttgatga cgccgtactg atgaacttcc tcgcgcggaa ctttcattag ggcaacgacc
     4621 gactcttcgg tttcggtgaa aatctcgact agttgccgga tccccggggt ctcggcatca
     4681 attaaatcat ccgccagcat gacagcgaaa ggctcgtccc cgaccaggtc gcgagcgcaa
     4741 agaacc
//
LOCUS       DASUVS010000345         3590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1239329, whole genome shotgun sequence.
ACCESSION   DASUVS010000345 DASUVS010000000
VERSION     DASUVS010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239329"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..394
                     /locus_tag="VF182_18305"
     CDS             191..394
                     /locus_tag="VF182_18305"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEX6439090.1"
                     /translation="MKRAPFVGFVKRSTSLQIPQVVTVRHILVHDYFVIDIDFVWDAV
                     ERDLPPLRDNIEGMLLEQQETNF"
     gene            complement(413..1423)
                     /locus_tag="VF182_18310"
     CDS             complement(413..1423)
                     /locus_tag="VF182_18310"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439091.1"
                     /translation="MFSKLAGKTVGLILASVIFLPALSAAQEKGLKKFHWGVTSLSAS
                     QWIPWIAKEAKLYEKYGMEAELVLLRGSGQSAQAIIGGSILGAPCTLTTVMGANLSGA
                     DLVTVAHTVSGVQSKLLVRADIKKPEDLRGKRIAVSGFGSLGDFLERYIIKKHGMDPV
                     KDMILLSIGTQPDRIQALFNGVVDAADLSHPADVQAERKGYRVLWDAKQEVSYPSMSI
                     VTRRKHTLDDRDSVMRMVKAHVEGIQLLKSNKEFSLKVLSKYLKTSDRELLEGSYEIY
                     RESFISVPYPITQGLQPTYEYVAQQRPDVWNYKPEAFMDASFIAELEKTGFIKNLSAG
                     AR"
     gene            complement(1473..2558)
                     /locus_tag="VF182_18315"
     CDS             complement(1473..2558)
                     /locus_tag="VF182_18315"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6439092.1"
                     /translation="MNGLRIIDADGHVQERDADWQELLDPPYRKHAPKMVTGSDGKES
                     LLLEGKIWAKPSGTGLGIGTAPYSRRPQKTTGMFDPVRRLQDMDLEGIDTAVNFGTTV
                     FLSLPFLEDYDLACAIAHGYNTWLDRYCKTSPQRLKGVALVAMQEPKEAVKELERCIN
                     ELGFVGVATSAHSAGRNLDHPDFYPFYAKAQELGVPVCVHVGSGRPAAAAERFNNAFF
                     VHATTHAFEQMIGVMCIVGGGILELYPKLKVAFLEAGAGWVPYWMERLDEHYEYLQPT
                     VPLMKRPPSDYMRSEQVYYSFEPDEHTLNFVMNYVGEDRLVFASDYNHGDSKFPRTVA
                     SVTERKDLSASQLRKLMCDNAARLYNL"
     gene            complement(2776..>3590)
                     /locus_tag="VF182_18320"
     CDS             complement(2776..>3590)
                     /locus_tag="VF182_18320"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6439093.1"
                     /translation="KVDSYRAQGADLVVRLEEEAKARRNIADTIETTEGSLIFLRDEK
                     LLRPYDSPYLDNYPEDGKERADKNLVYWALARESYIGFTYNKTLLPKDAVPKNFDGLF
                     HPQLKGKMGISIGQTSDKIIGAMIKSKGEEMVRKLKGQEMKLYSIDAPALVQTIAGGE
                     IAASPAIFQTHTLLAASKGAPVEWVPMDLVPTNVGSAAIAANPPHPHAALLMADFLLS
                     PAGQQVLEKYYYGSGAKDYGFKKWRPEHGLTTEQYEKDLERWDKLLNAITRK"
BASE COUNT          763 a          998 c          953 g          876 t
ORIGIN      
        1 atctcgtgaa atcgatcatc gccgatcagc agtttatgat cgaacagctc gtgcgttaca
       61 tcagcggata aaggattgca attccctagg agttataaag aaagaaaagc cgtggcttcg
      121 gtgaggtgct ctcatctcga agcgacgcaa attcgttgac cggttttccg gagttatctc
      181 aagccttatc ctgaagcgag ctccatttgt tgggttcgtc aagcgctcca cctccctcca
      241 gattcctcaa gtcgtcacgg tccggcatat ccttgttcac gactattttg taattgacat
      301 cgactttgtc tgggacgcgg ttgaacgaga cctgccgccg ttaagggata atatcgaggg
      361 aatgctgctc gagcagcagg aaacaaattt ctgatccagc tttctagtcc agctatcgcg
      421 cacccgcgct cagattcttt atgaagccgg ttttttccag ctccgcgatg aagcttgcat
      481 ccataaaagc ttccggcttg tagttccaaa cgtcggggcg ttgttgcgcg acgtattcat
      541 acgtcggctg caagccttga gtgatcgggt aggggaccga gataaaagac tcgcgataga
      601 tttcgtacga gccttcgagc aattcgcgat cgctggtttt caggtattta cttagcactt
      661 tgagactaaa ttctttattg ctcttcaaaa gctggatgcc ttcgacatgc gccttgacca
      721 tgcgcatgac gctgtcgcgg tcgtcgaggg tatgtttgcg ccgggtgacg atcgacatcg
      781 atggatagct gacctcctgt ttggcatccc acaagacgcg atagcctttt cgctcggctt
      841 gaacgtcggc gggatgagaa agatcggcgg catcgaccac gccgttgaat aaagcttgaa
      901 ttcgatcggg ttgcgtgccg atcgagagaa gaatcatgtc tttcaccggg tccatgccgt
      961 gtttcttgat gatgtaacgc tcgagaaagt ctcccagcga gccaaatcct gaaaccgcga
     1021 ttctcttgcc ccgaagatcc tcgggttttt tgatatccgc gcgaaccagc aatttacttt
     1081 gcacgcccga aaccgtgtgc gctacggtga cgagatccgc gccgctcaga ttggcgccca
     1141 taacggtcgt caacgtgcag ggcgcgccga gaatgctgcc gccgataatc gcctgggcgg
     1201 actgtcccga gccgcgcaat aacaccagct cggcctccat tccatatttt tcgtatagct
     1261 tggcttcttt ggcgatccat gggatccact gcgaagcgga tagagacgtg acgccccagt
     1321 ggaatttttt cagccctttc tcctgggccg ccgagagagc aggcagaaat atcactgaag
     1381 cgaggatcag gccgacggtt ttgccagcaa gctttgaaaa cattcgcgcc ctccttgttg
     1441 caggccggtt gcctaatgcc tcgttcatgc tcctaaaggt tgtagagtcg cgccgcgttg
     1501 tcgcacatca atttgcgtaa ttgactcgcg ctcagatcct tgcgctcggt caccgaggct
     1561 accgtccggg gaaatttgct atcgccatga ttgtaatcgg acgcaaagac caaacggtct
     1621 tcgccgacat aattcatgac gaagttcaag gtgtgctcgt ccggctcgaa tgaatagtac
     1681 acctgctcgc tgcgcatgta gtcgctgggc ggccttttca tcagcggcac agtcggctga
     1741 agatactcgt aatgttcgtc caaacgctcc atccagtatg gcacccagcc agcgccggct
     1801 tcgagaaagg ctactttcaa tttggggtac aattccagaa tcccgccgcc gacaatgcac
     1861 ataacgccga tcatctgctc gaaggcgtgg gtcgttgcat ggacgaagaa cgcattgttg
     1921 aagcgttcgg cggcggccgc cggccggccg gaaccgacat gcacgcacac cggcacgccg
     1981 agttcctgag ccttggcata aaatggataa aaatcgggat gatccaaatt gcgtcccgct
     2041 gaatgcgccg aagtagcgac gccgacaaag cccaattcat taatgcagcg ttccagctcc
     2101 ttcaccgctt ccttaggctc ctgcatggcc accaaggcga cgcctttcaa tcgttgcggg
     2161 ctggttttgc agtagcggtc gagccatgtg ttatagccgt gcgcgatggc gcaggccaga
     2221 tcgtaatctt cgagaaaggg caggcttaaa aaaaccgtcg tgccgaaatt aaccgccgtg
     2281 tcgatacctt cgagatccat gtcttgtaag cgccgcaccg gatcgaacat tccggtcgtt
     2341 ttttgtggcc ttcggctata gggtgccgtg ccgatgccta agcctgttcc agaaggcttc
     2401 gcccaaattt ttccttcgag aagcagtgac tccttgccgt cagatccggt aaccattttg
     2461 ggcgcatgct ttctgtaggg cgggtccagt agttcctgcc aatcagcatc ccgttcctgc
     2521 acgtgtccgt cggcgtcgat gatgcgaaga ccgttcatgt catgcctcct ctaatcgaga
     2581 agaagatagc cgcaaagaac ctaacgcggc agagccgcaa ccgaaaacga aaagcaggat
     2641 tccaccgcgg aggcacagag cacgcagtgt tttttatttt aaaactcccc tccgcgtcct
     2701 cagcgcctcg gcggtaaacc atccgactcc tcgggtatgg tgcccgatgt aggtaattga
     2761 ttctcttgtt tatttttatt tgcgggtgat cgcgttcaag agcttgtccc aacgctccaa
     2821 atctttttcg tactgctccg tagtcagccc gtgctccggg cgccactttt tgaatccata
     2881 gtctttggcg ccgctgccgt aataatattt ttccaagact tgttgccccg cggggcttaa
     2941 cagaaaatcc gccatcagca gcgcggcatg aggatgcggc ggattggccg caatcgcggc
     3001 gctgcccaca ttggtaggaa ccaagtccat cggcacccac tccaccggcg ctcccttgga
     3061 tgccgccaat agggtatgag tctgaaagat cgccggcgat gcggcgatct cgccgcctgc
     3121 gatggtttga acgagcgccg gggcgtcgat cgagtagagt ttcatctctt gacctttgag
     3181 cttgcgcacc atttcttcgc ccttagactt gatcatggcg ccgatgatct tgtcgctggt
     3241 ctggccaatg ctgataccca tcttgccttt gagctgcggg tgaaagaggc cgtcaaagtt
     3301 cttcggcacg gcgtctttcg gcagcagcgt tttgttgtag gtgaaaccaa tataggattc
     3361 ccgcgcgaga gcccaataaa caaggttctt atccgcgcgc tctttgccgt cttcagggta
     3421 gttatccaga taaggtgagt cgtagggccg gagcaatttt tcatcgcgca ggaatatcag
     3481 gcttccctcg gtggtctcga tcgtatccgc gatattgcgc cgcgctttgg cctcttcctc
     3541 caagcgcacg accaagtcgg ctccctgggc gcgataagag tccaccttga
//
LOCUS       DASUVS010000346         4266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_803563, whole genome shotgun sequence.
ACCESSION   DASUVS010000346 DASUVS010000000
VERSION     DASUVS010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803563"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VF182_18325"
     CDS             complement(<1..1076)
                     /locus_tag="VF182_18325"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6439094.1"
                     /translation="MPKNIKKGSQSNKVKPRHAAVKAPLSKKAQTIAEVRRELAESLQ
                     REKAYVNELQDVKRQLTEALKQQTATSEILSVIAGSPTDIQPVLDVVAENAARLCGAT
                     DAQINRVEGDLLRRVASYGSMATPDEVSVFHRGHVGGRAVIDRQTIHIQDITAGFDEE
                     FPGARTFYERTGVRTILATPLLREGDAIGNVVIRRTEVRPFTDKQIALLKTFADQAVI
                     AIENVRLFQELQDRNRDLSEALEQQTATSEILGAISSSPADLQPVFETILANATRLCG
                     TQNAALFRFDGEFLRFAAGHNLGPENMARMENYSVRPGTETASRRAALERRTIHVPDV
                     VAAAGFDAYQTDTYRRENRLSTLAV"
     gene            complement(1750..2784)
                     /locus_tag="VF182_18330"
     CDS             complement(1750..2784)
                     /locus_tag="VF182_18330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439095.1"
                     /translation="MTKAFCGFLLAVFISASLATSVIAASAEEFYKGKAIQFIVGGSA
                     GGGYDTYTRLIARYFSQYVPGKPSTVVQNMPGAAMLIAANFTFNSAPHDGTVIGHWSG
                     PLILQHLMGNPAVQFDGRKFGWLGMPTADSLICITTERSGIKTAEDWRKAKTPVKLGA
                     IGPGTSGTDDTKLLAAATGFPLQLIEGYKGTADIRIAAETGEVDGTCAFGWQSAKVTW
                     ANALKAREIHVVLQTMLEPHPELKNVPLAVDYAKTDEGKKLLQIASELYGKQRLYSLP
                     PQVPEERVRILQKAFIDTLKDPQLKAEAEKAKLEIDPIDGPGIEKMVKGLYEIEPAIL
                     KRVKQILESK"
     gene            3330..>4266
                     /locus_tag="VF182_18335"
     CDS             3330..>4266
                     /locus_tag="VF182_18335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX6439096.1"
                     /translation="MANISGKYCIVGIGETVVGKRPDATTNSLHLEAIKACLDDAAIK
                     NSQVDGLLTNQPLNDSHRSYAVKLAHMAGIDPLFATDLALGGATPIAMVQHAVMAIDA
                     GMASTVVCVHARKRSTPDPTPGHPIRRGDEHWEEPWGHFAAAGGHAFAASRHMHEFGT
                     RSEDLAEIAISTRKHASLNKNATMRKPINLEDHQNSRMIVAPLRLLDCSLESDGGGAV
                     MVTSADRARDFPKKPVAILGMGQHHPHFSLLDAPTLTTLGGKKSSEMAYKMSGLGPKD
                     MKFAQIYDCFTITAMITLEDYGFCKKGEGKDFVKNG"
BASE COUNT          955 a         1197 c         1189 g          925 t
ORIGIN      
        1 acggctaaag tgctgagccg gttctccctg cgatacgtat cggtctggta agcatcgaag
       61 ccagccgcgg cgacaacatc aggcacgtga atggttcgtc gctccagagc cgcgcgtcgg
      121 cttgcagtct ctgttccggg ccggaccgag taattttcca tacgcgccat attctcagga
      181 ccgagattgt gtcccgcggc gaatcgcaga aattcgccat cgaaccggaa taatgcggcg
      241 ttctgtgtgc cgcacagacg tgtagcgttg gcgaggatcg tctcgaagac cggctgcaag
      301 tcggccggcg aactgctgat cgcgcccaga atctcactgg tcgcagtttg ttgctccagc
      361 gcttcgctca gatcgcggtt gcgatcctgc aattcttgga acaggcggac gttctcgatt
      421 gcgatgaccg cctgatcagc aaaggttttt agaagagcaa tctgtttgtc cgtgaagggg
      481 cgaacttccg tgcgacggat tacgacattt ccaatcgcat cgccctctcg cagcaatggc
      541 gtagccagga tcgtgcgcac gccggtccgt tcgtaaaatg tcctagctcc cggaaattcc
      601 tcatcaaacc cagcagtaat atcctggatg tgaatcgtct gccgatcgat aacggctcgg
      661 ccgcccacat ggcctcggtg aaatacacta acctcgtcag gcgtagccat tgagccatac
      721 gaagccactc gacgaagaag atcaccctcg acacgattga tctgggcatc cgttgcaccg
      781 cacagccgag cggcattctc tgccaccaca tctagcaccg gctggatatc cgtcggtgag
      841 ccggcgatga cgctaagaat ttcgctcgtg gctgtctgct gctttaatgc ttcggtgagc
      901 tgacgtttaa cgtcctgcaa ttcattgacg tatgccttct cgcgctgtaa cgattcggcg
      961 agttcgcggc gcacctcggc aatcgtttgc gctttcttag atagcggcgc cttcactgcc
     1021 gcatggcgag gttttacttt attgctctga gaaccttttt ttatgttctt tggcataagt
     1081 aaaagagcga actaagatat cgctcgggga gaaaaaatgc gtggagagga tcggggtgcg
     1141 ccgcggaaac agctccccag cggctcagct aagtcttact tcaggtgggc tgttggcgtc
     1201 aacgaagaaa ttcatgattg cgagcaaggc gcctctgcca atactcacat ccgaaatcca
     1261 ttgctgccgc aaaagcacga gccggtatag aacaatgaca agcttcaggc aatcctacag
     1321 atctgcccga tggatattca actcaccgct agggtgatgg cgagccaccc cgaaacaatg
     1381 ttcgacacgg aatgttcggc gaaaccctat ctgatctccg ggcgattgcc tcagcagtaa
     1441 gtccgcttcc cgatgtgcca atcacgattc ccgatttacg gggaagtgtg ttatcagctt
     1501 acactcgccc ccagagaatc aaaatggaat acgtccttca accgctaaca ctcggacgag
     1561 tctgtcattc ccgcatgctt cgagcgggaa tccaggcggg attcaaactc gacccccgat
     1621 taaaacattc gggggtgaca atattgggga gtcccagctc ttcacctcag cgacaatttc
     1681 cgaaggagac catcgtcaat caagacactc gatgccgtcg gttcaagttt tagcagaagt
     1741 tctccgctcc tactttgatt ccagaatttg tttaactcgc ttcaggatgg cgggctcgat
     1801 ctcatacaag cccttcacca tcttttctat gcccggtcca tcgattgggt cgatctcgag
     1861 cttcgccttt tctgcctcgg ccttgagctg aggatccttg agagtatcga tgaaggcttt
     1921 ttgcagtatt cgcacgcgct cttcggggac ttgtggcgga agcgaataga gccgctgttt
     1981 gccgtaaagc tcgctcgcta tctggagaag ttttttgcct tcgtcggtct tggcgtagtc
     2041 gactgcgaga gggacattct tcagctcggg atgaggttcg agcatcgtct gcagcacaac
     2101 atgaatctcg cgtgccttca gcgcgttggc ccaggtcacc ttcgccgatt gccagccaaa
     2161 ggcgcaggtc ccgtcgacct cgccggtttc cgcggcaatc ctgatatccg cggtaccctt
     2221 gtacccctcg atcagctgca ggggaaaacc cgtggcggcg gcaagaagct tggtatcgtc
     2281 ggtgccgctg gtgccggggc caatcgcccc cagcttgacc ggtgttttag ccttgcgcca
     2341 atcctccgcc gttttgattc cgctgcgttc ggtggtgatg catatgaggg aatctgccgt
     2401 cggcatcccg agccagccaa atttgcgccc gtcgaattgc accgcagggt tccccatcag
     2461 gtgctgtaga atgagcgggc cggaccaatg cccgatgacc gttccgtcgt gaggagcgct
     2521 gttaaacgtg aaattggccg caatcagcat ggcggctccg ggcatattct gaacgacagt
     2581 ggaaggtttg cccggaacgt attgagagaa gtagcgcgca atcagcctgg tgtacgtatc
     2641 ataacctccg ccggcggaac cgccgacgat gaactggatt gctttgccct tgtagaactc
     2701 ctcggcggag gcagcaataa ccgacgtcgc cagcgatgct gagataaaga cagcaagtaa
     2761 aaatccacag aatgctttgg tcatgactta cctcccggtg atttgtctat tctggccgga
     2821 ggctaagttg cccgcggggg tcatgtcaag cagttttaga gtggaataaa cggcgataat
     2881 ggaatccaga tcaccccgaa gccgtcagaa ttgaactgat cgacgcgtca gctcaaatca
     2941 agggaagact gcttcgaagg agaaatcagt tcaaccctaa cgctaaacct ttgattgagc
     3001 cgtggcttgc gagcgtcgcg ctcgaattca tccttcgcca agaagacgaa gatcgatggc
     3061 ttaactgcgc cgcgaatgct ttcgacaggt tcgattgttg taaaagcatt tcgcgctgat
     3121 atcatggcca cgcacgagtt ttcccggagg gtgataaccc gaaatatgac gcgccggaat
     3181 gtagcgcgcc gttggaagtt gacgccatga tgaagaatca ttcaggcgtt gagattgaac
     3241 tcacgagtaa gaccggcgaa aatttgctgg agttaccgcg ttaacccgat cgacgagtga
     3301 cgccatcgaa catgtgacgg agaaaattca tggccaatat cagcggaaaa tattgtattg
     3361 tcggcatcgg cgagacggtc gtcggaaaac gtcccgacgc cacgacaaac tcgctccacc
     3421 tcgaggctat taaggcatgc ctcgacgacg cggcaatcaa aaactcgcag gtcgacggcc
     3481 tgctcaccaa tcagccgttg aacgattcgc accggagtta cgccgtcaag ctggcccata
     3541 tggcgggcat cgatccgctg tttgccacgg accttgcgct cggaggcgcg acgccgatcg
     3601 cgatggtgca gcatgcggtg atggcgatcg acgcaggcat ggcgagcaca gtggtctgcg
     3661 ttcatgcgcg caagcgctcg acgcccgatc cgaccccagg ccatccgatc cggcgcggcg
     3721 acgagcattg ggaggaaccc tggggtcact tcgcggcggc cggcggccac gcctttgcgg
     3781 cgtcgcgtca catgcatgaa ttcggcacga gaagcgagga tctggccgag atcgcgatct
     3841 ccacgcgcaa gcacgcgagc ctgaacaaaa acgcgactat gcgcaaaccg atcaatctgg
     3901 aagatcacca gaactcgcgc atgatcgtag cgccgctgcg attgctcgat tgttctctcg
     3961 aatcggacgg cggcggcgca gtcatggtga ccagcgccga ccgcgcgcgc gactttccga
     4021 agaagccggt ggccattctg ggcatgggcc agcaccatcc gcacttcagt cttttggacg
     4081 cgccgacgct aaccacgctg ggcgggaaaa aatcttctga gatggcgtac aagatgagcg
     4141 gtctgggccc gaaagacatg aagttcgcgc agatctacga ctgtttcacg atcacggcga
     4201 tgatcacgct cgaagactac ggtttttgca aaaagggcga aggcaaagac ttcgtcaaga
     4261 acggac
//
LOCUS       DASUVS010000347         3904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_804581, whole genome shotgun sequence.
ACCESSION   DASUVS010000347 DASUVS010000000
VERSION     DASUVS010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804581"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /gene="pgsA"
                     /locus_tag="VF182_18340"
     CDS             complement(<1..519)
                     /gene="pgsA"
                     /locus_tag="VF182_18340"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX6439097.1"
                     /translation="MLNLPNFLTLIRILSIPFFLVYLSYHRYLEALIIFIIGGITDFL
                     DGFVARLMKQQTPLGAYLDPIADKLLVITSFIMLGVIDGIPMWLAIIVVSRDVLIITG
                     YGIILFLTDERPEVKPSLIGKWSTLLQLLTLGIALALLHNPKLMNHWVLDVFIGFTAV
                     ATVLSGLQYIYRG"
     gene            complement(526..1824)
                     /gene="hflX"
                     /locus_tag="VF182_18345"
     CDS             complement(526..1824)
                     /gene="hflX"
                     /locus_tag="VF182_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX6439098.1"
                     /translation="MRPHSRQQRKERAVLVGVELPSHDHKISLEYSLEELQSLAQTAG
                     AEVAEKFTQQLRSITPATLVGRGKVDQIHARVDELHPDLVIFDEDLTPAQQRNLELAL
                     NVRVIDRSQLILDIFAQRARSNEGKLQVELAQLEYLLPRLTRQWTHLSRLGGGIGTRG
                     PGETQLEVDRRRIREKISHLKRRLKTVERTRSLQRKERDNVPFATIALVGYTNAGKST
                     LMNALTRADVLVEDKLFATLDPTIRSLRLPNGDKVMVADTVGFINKIPHSLIEAFKST
                     LEEVMSADLLLHLVDMSSPLVDEQIQVIEEVLGEIGAGDIPTIIVPNKIDLLSEPSDH
                     LFKNRRSEEVCPISAVTGDGLEALLKVIGTQLDRGKEKIHVSLSMGQGDLLSLLRERG
                     RVENEIYEDDQVHITAMVTPKLAGQLRKLALNNTSSDRKR"
     gene            complement(1824..>3904)
                     /gene="dnaE"
                     /locus_tag="VF182_18350"
     CDS             complement(1824..>3904)
                     /gene="dnaE"
                     /locus_tag="VF182_18350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX6439099.1"
                     /translation="TESMKMEPRLPELMQSDPRVKTLIEHALRLEGLVRHASTHAAGV
                     VLSNLPLVDHLPLFVDKEGGIVTQFDMSCVEKIGLVKFDFLGLKTLTLIHDCLKLIES
                     TQGVKIDLERLPLDDKKTYRLLCNGNTTGIFQLESTGIREMTVKIRPNCFEDLVAILA
                     LYRPGPLDSGMAEEYIKRKHGKEKFKYLHPHLEPVLKDTYGVIVYQEQVMQIAQILGG
                     YTMGDADILRRAMGKKDPEEMAAQRERFVEGARKKNIDDKTATEIFDQMETFARYGFN
                     KSHSAAYALVSYQTAYLKSHYPVEFMAALMTSEMGDTDKIIKNLAECREKGIEVLAPD
                     INESRSDFTPVGGKVRFGLAAVKSVGEKAVEVILESRRRDGPFQSLFDFCRRVDLTAV
                     NRRVVENLINCGAFDSTQVSRARMIAALDEAIRAGQAHQRDQASNQIDIFSLLGTPGK
                     GPNRPGDVYPQVKEWSQQELLAFEKEALGFYITGHPLDKYDRVIKRIISGTAAGIKER
                     PVAGEVKLAGVVSALKLRNTKKGDRYGSFNLEDKTGFLEVIAWPEIYKKSAELLGSDD
                     PIFIKGKLEVGEDRMQIIANEITRLAEAAAKLRPLANGNPHRNGSIERVHLYVRESEI
                     SAQELVQLREALLNCPGTCTVVLHLLAEDPNETVIELPDQIRVAPGPQLGAIVSKLFG
                     PRISFRSLAS"
BASE COUNT          885 a         1114 c          961 g          944 t
ORIGIN      
        1 gccgcggtag atgtactgca agccggagag cacggttgcc actgccgtaa agccgataaa
       61 aacatcgagc acccagtgat tcatcagctt ggggttgtgc agcagagcca aagcgatacc
      121 caaggtcaag agttgtaata gagtgctcca tttgccaatg agactcggct tcacctccgg
      181 acgttcatcg gttaagaaaa gaatgatgcc gtagccagtg atgatcagga cgtcacggct
      241 gacaacgata atcgccagcc acatgggaat tccgtcgatc acaccgagca tgataaaaga
      301 agttatcacc agcaacttgt ccgcgatggg atcgagataa gctcctaaag gagtctgctg
      361 cttcatcaac cttgccacga agccgtcgag gaagtcagtg atgccaccga tgataaaaat
      421 gatcagcgct tccaggtaac gatgatagga aagatagact agaaagaaag ggatgctcag
      481 aatcctaatc agggtcagaa agttagggag atttagcatc cgtatctaac gcttgcgatc
      541 gctcgaggtg ttgttgagcg ccagttttcg caactgccca gccaacttcg gtgtcaccat
      601 cgcggtgata tgaacctgat catcctcgta aatctcattc tctactcgcc ctcgctctcg
      661 caggagggac aacaaatctc cctggcccat tgaaagcgat acgtggatct tttctttgcc
      721 tcgatccaac tgcgtgccaa taaccttcag aagcgcctcg agtccgtcgc ccgtcacggc
      781 ggaaatcgga cacacctctt cgctgcggcg atttttgaac aggtggtctg atggttcaga
      841 cagcaagtct atcttgttgg gaacgatgat ggtcggaatg tcgccggcgc caatttcgcc
      901 gagcacttct tcgatcactt gaatctgctc gtcgaccagt gggctgctca tgtcgactag
      961 gtgcaacagc aaatcagcgc tcatcacttc ttcaagtgtg ctcttgaatg cctcgatcag
     1021 cgaatgcggg atcttgttga taaaaccgac agtgtctgcg accatcacct tgtccccatt
     1081 gggcaggcgt agtgaccgaa tcgtcggatc caaagtcgca aataatttat cctcgaccaa
     1141 aacatccgcc cgcgtgaggg cgttcatcaa ggtcgattta ccggcattcg tgtaaccaac
     1201 aagtgcgatc gtggcaaacg gcacattgtc acgctctttc cgctgcaaag aacgggtccg
     1261 ctccaccgtt tttagccgac gcttcaaatg acttattttc tcgcgaatgc gccgccggtc
     1321 cacttcaagc tgggtttcac ccggtccccg tgtgccgata ccgccgccga gccgcgaaag
     1381 atgtgtccat tggcgcgtta ggcgcggcag caaatattct agttgggcca gctcaacctg
     1441 cagcttacct tcgttgcttc gcgccctttg agcaaaaata tcgagtatta attgactgcg
     1501 gtcgatgacc cgcacgttga gagccaattc caaattgcgc tgctgagccg gagttaaatc
     1561 ctcatcgaaa atcaccaggt ctggatgcag ctcgtctact ctggcatgaa tttgatcgac
     1621 tttgccccgg ccgaccaatg tagccggggt aatgcttctc agctgttggg taaatttttc
     1681 cgctacttcg gcccccgccg tttgagctaa actctgaagc tcctcaaggc tgtattcgag
     1741 agagatttta tgatcgtgtg acggcagttc gacgccgacc aaaacggcgc gctctttgcg
     1801 ctgctgcctc gaatgcggcc tcatcaagac gctaacgaac gaaagctgat ccgcggaccg
     1861 aagagctttg aaacaatcgc tcctaactgc gggccgggag cgactcggat ttgatccggc
     1921 aactcaatta cagtttcgtt aggatcttcg gccaggagat gaagaacaac cgtgcaggtt
     1981 ccggggcaat tcagtagagc ctcccgcagt tgaaccagtt cttgcgcgga aatttccgat
     2041 tcgcgcacat aaagatgcac tctttcaatg ctgccgttgc ggtggggatt gccattggcg
     2101 agggggcgaa gtttcgcggc cgcctcggcc aatcgagtta tttcattagc gattatctgc
     2161 atgcgatctt cgccaacctc aagttttcct ttaataaata tcggatcatc cgagcctaaa
     2221 agctccgcgc tttttttgta aatctccggc catgcgatca cctccagaaa gccggttttg
     2281 tcttccaaat tgaagctgcc gtagcgatcg cctttcttgg tgtttcttaa cttcagcgcc
     2341 gacactaccc cggccagctt tacctcgccg gccaccggcc tttctttgat cccagccgca
     2401 gtgccgctga tgattctttt tatcacccga tcgtacttat ctaaagggtg tccggtgata
     2461 tagaacccca gcgcttcctt ctcaaaggcc aacagctcct gctgtgacca ttctttgacc
     2521 tgtggataaa catcgcccgg acggtttggc cctttgccgg gagtgcccag caggctgaaa
     2581 atgtcgattt gattgctcgc ttgatcgcgc tgatgcgcct ggcccgccct tatagcctca
     2641 tccaaggcgg cgatcatgcg cgcccgtgaa acctgggtcg aatcaaaggc gccgcagtta
     2701 atcagattct caaccacccg ccggttcacc gctgttagat cgacgcggcg acagaaatcg
     2761 aaaagcgatt gaaaagggcc gtctcgccgg cgactctcca gaatgacctc cacggccttt
     2821 tcgccgacac tctttactgc tgccaacccg aagcgcacct tgccgcccac cggagtaaaa
     2881 tcggaccggc tctcgttgat atcgggcgca agcacttcga tccccttctc tctgcactca
     2941 gcaagattct tgatgatctt gtccgtgtcc cccatctctg aagtcatcaa cgccgccatg
     3001 aactcgaccg gatagtggct cttcaaatag gccgtctgat acgagacgag cgcatacgcc
     3061 gccgagtgtg atttattgaa accataccgg gcaaaggtct ccatctgatc gaagatctcc
     3121 gtggcggttt tatcgtcgat gtttttcttg cgcgctcctt cgacgaacct ttctctctgg
     3181 gcggccatct cctcaggatc ctttttgccc atggcgcgcc gcaggatatc ggcatcgccc
     3241 atcgtataac cgccgaggat ctgagcaatc tgcatcacct gctcctgata gacgattaca
     3301 ccgtaagtat ctttaaggac cggttccaaa tgaggatgca aatacttgaa cttttctttg
     3361 ccatgcttgc gcttgatgta ttcttccgcc atgccgctgt ccaaaggccc cggccgatac
     3421 agcgctagga tcgccaccag atcttcaaaa caattggggc gtatcttgac ggtcatttct
     3481 cgaatcccgg tgctttccag ctggaatatc ccggtggtat tgccgttaca gagcagccgg
     3541 taggttttct tatcatccag tggcagtcgc tccaaatcga tctttacgcc ctgagttgac
     3601 tcgatcagct tcaggcaatc atgaatcagc gtcagcgtct tcagaccgag aaagtcaaat
     3661 ttgaccagcc cgatcttttc cacgcagctc atgtcgaact gagtgacgat gccgccttct
     3721 ttatcgacaa acaacggcag atgatcgacc agtggcaaat tcgaaagcac gacgcccgcc
     3781 gcgtgcgtcg aagcatgtcg caccaggcct tctaaacgta gcgcgtgctc gatcaaggtc
     3841 ttgacccgcg gatcgctctg catcaattcc ggcaggcgcg gttccatttt catggactcc
     3901 gtca
//
LOCUS       DASUVS010000348         5678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1020756, whole genome shotgun sequence.
ACCESSION   DASUVS010000348 DASUVS010000000
VERSION     DASUVS010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020756"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VF182_18355"
     CDS             <1..674
                     /locus_tag="VF182_18355"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439100.1"
                     /translation="TGNVSPFRLMARPEIKQPTDLKGKRAGITTFGSTSDQVVRIALQ
                     KFNLEPNKDVAILSLGAQPEAFAALQSGAVQVAALSYPLYAKAAQLGMRELINFGDLG
                     VEDVNGGVITTRSFIAQHRDTATRFVRAYIRGAHRYRTDKEFSKRVLGKYGKITEDDL
                     LEGTWQDYAPTIQKVPRPSPKAIQFLIEFQFKGKTPLPKPENFVDMSIVEELEKNSFI
                     DSVYK"
     gene            complement(851..1162)
                     /locus_tag="VF182_18360"
     CDS             complement(851..1162)
                     /locus_tag="VF182_18360"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6439101.1"
                     /translation="MIRNLKSIGLVLVLLGILGGCQAMTGQTAGENVDDATITSTVKA
                     RLAQDKLGTLTRVDVDTTNGVVALNGVVATAQDRSRAEEVARAVGGVKKVTNNLQVQK
                     K"
     gene            complement(1283..2404)
                     /locus_tag="VF182_18365"
     CDS             complement(1283..2404)
                     /locus_tag="VF182_18365"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEX6439102.1"
                     /translation="MVRVNKPPFRADHVGSLLRPPELLQARELRAKGTISDKELRLTE
                     DRCIRDVVKMQEDIGLHGITDGEYRRTLWHADFLSRIEGVVVKEGIAPGAGRDFQGGD
                     DVQRSPTRFETAGKLRRRSGLEIENFKFLKSVVRETAKLSIPSPSLLHFRGGRGAVDQ
                     QAYPDMAEFFADLAGVYREEIAALAQAGCTYLQIDDTNLAYLCDPKIRDSAKKIGEDP
                     DKLPALYAGLINECIKDRPATMTVCTHLCRGNFASAWVAEGGYDPVAEVLFNELRVDG
                     YFLEFDSPRAGNFAPLRYLPPHKRLNLGLVTTKTGALETADDLKRRIGDASKIVPLEQ
                     LGISPQCGFSSTVLGNKLTIQEQVAKLRLIVEVAKDIWG"
     gene            complement(2476..3378)
                     /locus_tag="VF182_18370"
     CDS             complement(2476..3378)
                     /locus_tag="VF182_18370"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6439103.1"
                     /translation="MFSRMLIPLDGSKTAETVLPYARRLAAVAHVAVELLGVVEMADI
                     AGDIDSNQVTYAKALIGEAVRNSTEYLESLAKTFPNGQVSCSVEQGRPEEVITGVAAA
                     ESNTLIAMATHGRSGVTRWLLGSVTEKVLRGTVNPLLVVRAPGDSKTEGEAVLSSVIV
                     PLDGSEIAESVLLPVAVLAKALKMQVILVRVYGVPLATYGGDDYYVPDYLELKDQIKD
                     AADSYLNSRASLLRQQGVAEVSTVVIEGSAADAIISLARKTPDNLVAMSPYGRSGLQR
                     WVLGSVTEKVVRHCEDPVLIVRGA"
     gene            3710..4048
                     /locus_tag="VF182_18375"
     CDS             3710..4048
                     /locus_tag="VF182_18375"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX6439104.1"
                     /translation="MQSNIEPTYAARLRSRRIEVEKTLGHLSTERRQVESNTEWLNYT
                     AYKRRITLLDRVTAWYRSEMAQIDKALTRVKDHRYGVCAACHQPIAMEQLELSPESEH
                     CPSCQSLQEH"
     gene            complement(4055..5641)
                     /locus_tag="VF182_18380"
     CDS             complement(4055..5641)
                     /locus_tag="VF182_18380"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6439105.1"
                     /translation="MQHTDAREQEPNRQNELIAAMLQPGFYPVRPAEVTHKETHISHL
                     FFAADLVYKIKKAVRFSFLDYSTLAQRRYYLDQELTLNRRLAPSVYLEVLPIGWDVSG
                     WRLGAGDNPVEFALMMRRLPDSRMLTSLLQSSQVTVDMMRSLAQVLASFHADAKRTNS
                     AGAAAHFDTVTKQWRDNLKDVEQLIGSAVDVESFTAIKAFGDHFLDEYREFISQRAFD
                     GWVRDGHGDLHCEHVCFAPEGIQIFDCIEFDSQLRLCDLASEIGFLLMDLEARGGAPL
                     GEALLARYRDLIDDSSLIVLLPFYKCYRALIRGKVEVLRAGASAASVRYFTIAGRQTW
                     SPYKPFVIVVCGLTGSGKSTLARELSDRIGIAVINSDGVRKELTGRSGRGVPFNQGIY
                     SPEITESTYGRIFDETDRRLSHGQSVMLDATFAQRRHRENLVGVAARHRVPVYFLHCV
                     ASDSLTHARLRERAMNENEVSDGRWEIYLEQKSIAEPLDECSAEVYLELPADRSIEDL
                     RRSSEGFLRGRLSRTTGHPG"
BASE COUNT         1304 a         1642 c         1461 g         1271 t
ORIGIN      
        1 cgaccgggaa cgtatcaccg tttcgtttga tggcgcgacc cgagatcaag cagcctacag
       61 atctaaaggg caaaagagcc ggcatcacca cgttcggatc gacatccgat caggtggttc
      121 gcatagcgct gcagaagttc aatttggagc caaataagga tgtcgcgatt ttatctttgg
      181 gcgcccaacc ggaggcgttt gccgcattac agagcggtgc ggtgcaagtg gcggcgctaa
      241 gctatccgct ctacgccaaa gccgcccagc taggaatgcg cgagttgatc aacttcggtg
      301 atcttggagt ggaagacgtc aatggcggcg tgattaccac ccggtctttc attgcgcagc
      361 atcgtgatac tgcgacccgc ttcgtcagag cttacatacg cggcgcgcac cgttatcgta
      421 cagacaagga atttagcaag cgtgttctag ggaagtacgg caagattacc gaagatgatc
      481 ttttggaagg cacgtggcag gattatgcgc cgactattca aaaagtccca cgcccctccc
      541 caaaagccat tcagttcttg attgaatttc agttcaaagg caagacaccg ctgcccaaac
      601 cggaaaactt tgtcgatatg tctatcgtgg aggaactcga aaagaacagc tttattgatt
      661 cggtgtacaa atgacgggca gcagatagcc cgacgccgac ccgctagtaa tcgtatggcc
      721 gaggacggga tctgattggc cacgaaaact cgccgccaac ggtaagggga tcgtttctgg
      781 attctccgcc agatgcggcc ccacgcacca actcacgtga ggccgcctag cagtcactag
      841 caccgtatgg ctacttcttc tgaacctgga gattgttggt aacttttttt acgccaccaa
      901 cggcccgcgc tacctcttcc gcccgggatc tgtcctgcgc cgtagcaacg actccgttta
      961 gggcaaccac accgttggtg gtatcgacat cgacacgggt caacgtaccg agcttatcct
     1021 gggccagtcg agccttcacc gttgaggtga tggtcgcgtc atcgacattc tcgccggcgg
     1081 tctggccggt catcgcctga catcctccga gaatcccgag cagcacgagc acgaggccaa
     1141 ttgattttag attccgtatc atagttgaat cctccttttt tatttttaac aaccacggag
     1201 ggttgaagca actagggtgc acgcggtcgc cagacatagt cgctcactgc cagggagtct
     1261 agagtggcta ccccgaagct ccctaccccc agatatcttt ggctacttcg acgatcaatc
     1321 tcaatttcgc gacctgctct tgaatggtca gcttattacc cagcaccgtg ctggaaaaac
     1381 cgcattgcgg gctgatgcca agttgttcca aaggcacgat cttgctggca tcaccgatgc
     1441 gacgcttgag gtcgtcggct gtctccagcg cccctgtctt agtggtcacg aggccaagat
     1501 tcagtcgctt gtgaggcggt agataacgga gcggcgcgaa gtttccggcg cgcggtgaat
     1561 cgaattccag aaaataaccg tcaaccctga gttcgttaaa aagcacctcg gcgaccggat
     1621 catagccgcc ttcggcaacc caggcgctag cgaagttacc tcggcacagg tgcgtgcaaa
     1681 ccgtcatcgt cgccggtcga tctttgatac attcattgat caagccggca taaagggcgg
     1741 gcaatttgtc gggatcttca ccaatttttt tggcgctatc gcgtattttc ggatcgcaca
     1801 aataagcaag attggtatcg tcgatttgca gataagtaca gccggcttga gccagcgcgg
     1861 caatttcttc gcggtaaaca cccgccagat cagcaaagaa ctcagccata tcaggatacg
     1921 cctgctgatc gaccgctccg cgtccgccgc gaaagtgcag caacgaaggc gaaggaatcg
     1981 agagcttcgc ggtctctctt accaccgatt tgagaaattt aaagttctcg atctccagac
     2041 cgctccgccg ccgcaatttc ccggcagttt caaatcgtgt cggcgaccgc tgcacgtcat
     2101 cgccaccctg gaagtcgcgc cccgcgccag gtgctatccc ctctttcaca accacacctt
     2161 cgattctgct gagaaagtcg gcatgccaca acgtccggcg gtactcgcca tcggtgatgc
     2221 cgtgaaggcc gatatcttcc tgcatcttga ccacatcgcg aatgcagcgg tcctcggtta
     2281 atcggagctc cttatctgag atagtgcctt ttgctctcag ctcacgcgcc tgcaaaagtt
     2341 ccggcggccg cagtaggctg ccgacatggt cggcgcgaaa cggtggcttg ttcactcgaa
     2401 ccattctgtt ctcctcggga attattcgat ctcttacttt gccggtcgtt ggcagcgtaa
     2461 accttctgtt tgcgcctatg ctcctcgcac tattaacact ggatcttcac aatgccggac
     2521 gaccttttca gtgacgctgc ccaacaccca ccgctgcaat cccgaacggc cataaggaga
     2581 catggccacg agattgtcgg gcgtcttgcg ggccagactg atgatggcat cggcggctga
     2641 accctcgatt accacggtag aaacctctgc cactccttgt tgccgtaaca aactagccct
     2701 ggagtttaaa tagctatcgg cggcgtcctt gatctgatct ttgagttcga gatagtcagg
     2761 cacgtagtag tcatctccgc cgtacgtcgc cagcggcacc ccgtaaactc gcacgagaat
     2821 cacctgcatc ttcagagcct tggccaaaac cgcaacaggt aaaagaacgc tttcggctat
     2881 ctccgaaccg tcgagcggca cgataaccga actcaagact gcttccccct ccgtcttcga
     2941 atcgcccggc gcgcgcacta ccaagagcgg attgaccgtg ccgcgcaaga ctttttcagt
     3001 gacgctgccc aatagccagc gagtcacacc cgaacggccg tgagtcgcca tggcaatcag
     3061 agtattgctc tcggcagccg cgaccccggt gataacctcc tcgggccggc cctgctccac
     3121 actgcagctt acttgaccgt tgggaaacgt cttcgctaag ctttccaaat attccgtgct
     3181 gttccgtaca gcttctccaa tcagcgcttt tgcataggtg acttggttgg agtcgatgtc
     3241 tccggcaatg tcagccattt cgaccacacc gagcaattcg accgcgacgt gcgcaacagc
     3301 cgcgagacgc cgggcgtagg gcaacacggt ctccgctgtc ttcgagccgt ctaacgggat
     3361 cagcattcgg ctaaacatac ttcctccttg atggaattac ttatttaccg ctgctgagtc
     3421 attgccacag agaaatagga ttcatgtatc aggtttgcaa attcattgag gcaataattc
     3481 tcatatccga gacatcggcg cagcaccttg ccgtgcaaag cccactaacg gaagaataag
     3541 attcggccat ttatggcatc ttttttgcgg cataatcgct cagtcggtgc agttgtacca
     3601 tcttcctgac agggatcgca cggcctggcg tgtctcttaa tccaatgtgc aaattttctc
     3661 tgattatctt ttttaacgaa ttttttaaga acggaaggag accgctgcga tgcagagcaa
     3721 tattgagccg acttacgcag cacggttgag aagcaggcgt atcgaagtcg agaaaactct
     3781 gggacatctt tcgacagaga ggcgccaggt cgaaagcaac accgagtggc tgaactacac
     3841 cgcatacaag aggcgtataa ctctccttga ccgcgtcacc gcgtggtacc gtagtgagat
     3901 ggctcagatc gataaagcat tgacccgggt caaggaccac cgttacggtg tttgtgctgc
     3961 ttgccatcag cccattgcaa tggaacagct agagctttcg cctgaatcag agcattgccc
     4021 ttcctgtcag tctctgcaag agcactgaag ccgatcagcc cggatggcca gtggtacgcg
     4081 acaatctgcc tcgcaagaac ccttcactcg agcgcctgag atcttcaatg gacctgtccg
     4141 ccggtaactc gagatagacc tcggcagagc attcgtcgag cggctccgca atcgatttct
     4201 gctctaggta gatttcccag cgtccgtccg aaacctcatt ttcattcatt gcacgctcac
     4261 gtaaacgagc gtgagtgagg ctgtcagatg cgacacaatg caagaaatac acgggtacac
     4321 gatgccgcgc cgcgacgcca acgaggttct cgcggtgccg cctttgcgcg aaagtggcat
     4381 ctaacatcac gctttgaccg tgcgagagtc ttcgatcggt ttcgtcgaat attcttccat
     4441 aggtgctttc ggtgatttcg gggctataga ttccctgatt aaaaggcact cccctgccgc
     4501 tcctacctgt aagctctttg cgcaccccat ccgagttgat cacagcgatc ccgattcggt
     4561 cacttagctc acgagccaag gtcgacttgc cgctgccggt caaaccacaa accacgatca
     4621 caaacggctt atacgggctc caggtctgcc gtccggcgat tgtgaaatat cttaccgacg
     4681 cggcgctcgc gccggcgcgt aatacttcga ccttgccgcg aatcaatgcg cgatagcatt
     4741 tatagaaggg cagcaacacg ataagactgg aatcatcgat gagatcgcga taacgagcca
     4801 gcaacgcttc gcccaagggg gcgccgcccc gcgcctccag gtccatcagc aaaaagccga
     4861 tttccgaggc aagatcgcac aaccgcaatt gggagtcgaa ttcgatgcag tcgaagatct
     4921 gaattccttc cggtgcaaag cagacgtgct cgcaatgcaa atcgccgtgc ccgtcccgca
     4981 cccagccgtc gaatgcgcgc tgcgagataa attcccggta ttcgtcgaga aaatggtccc
     5041 cgaaggcttt gatagctgta aagctctcga cgtcgacagc actgccgata agctgttcca
     5101 cgtccttaag attatcccgc cactgctttg tcactgtatc gaagtgggcg gcggcgccgg
     5161 cggaattcgt ccgtttggcg tcggcatgaa agctagccag gacttgcgcg agagagcgca
     5221 tcatgtccac tgtgacctgc gatgactgca gtaaggacgt caacatgcgg gagtccggta
     5281 aacgccgcat catcagcgcg aactccaccg gattatcgcc agctccaagg cgccatcctg
     5341 atacgtccca cccgatcggc agcacttcaa gatacacgga tggggctaag cgccgattga
     5401 gagtcagctc ttgatcgaga tagtagcgtc gctgggcaag cgtcgagtag tccagaaagg
     5461 aaaagcgaac cgcctttttt atcttgtaaa ccagatcggc ggcgaaaaag agatgcgaga
     5521 tgtgggtttc tttgtgagtc acttccgccg ggcgcaccgg atagaagccc ggctggagca
     5581 tcgctgcaat taattcattt tgccgattcg gttcctgctc ccgagcatcc gtgtgttgca
     5641 ctttatcctc ggaataaact tcgcactaaa tatgctac
//
LOCUS       DASUVS010000349        10939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_148477, whole genome shotgun sequence.
ACCESSION   DASUVS010000349 DASUVS010000000
VERSION     DASUVS010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10939
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148477"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..971
                     /locus_tag="VF182_18385"
     CDS             162..971
                     /locus_tag="VF182_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017321392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX6439106.1"
                     /translation="MSHDEVIGLIPAGGLGTRLAPLPCSKELYPIGFRESPGQGGWRP
                     KVVCHYLLENMRRAGITKAYIVLRDGKWDIPAYLKSGAIVDMHLSYLVLALPFGAPFT
                     LDEAYPFIRQSVVAFGFPDMIFQPEDAFSQLRSQQASSDASVLLGLFPSMEPDKVDMV
                     DIEPDGRVVKIDIKPARTDLTYTWGIALWRPIFSDFMHEYVSERKSDAATQPEITVGN
                     VIQAAIEAGLKVDGSPVSTEPYLDIGTSAGLILAIQKHCSSSPRASGRNSI"
     gene            968..1915
                     /locus_tag="VF182_18390"
     CDS             968..1915
                     /locus_tag="VF182_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017291437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6439107.1"
                     /translation="MTTNFVESFSQYVNRKVLLTGATGFIGAPLCRRLIEHGAEVYGI
                     SRSAQEPAASGMRWFQAGPADPCKIRQILSETKPDIIFHLAGMATAATELDLVVPTFD
                     SLLASTVYLLTSAAELGCGRIVIPGSLTEPKEDDVDATPGSPYAAAKWAGSAYSRMFF
                     KLYGTPVVIARPFMTYGPGQPRSKLVPYVILAALRREAPKISSGQWQADWIYIDDVVD
                     GFMAAGCTPGICGKTIDLGSGALTSVRAVVQKIIDLTDQEVHPIFGAQRDRRFEEVRV
                     ADTEFAYATLGWKSTISLTEGLARTVDWLREHVNQNRTS"
     gene            2192..2923
                     /locus_tag="VF182_18395"
     CDS             2192..2923
                     /locus_tag="VF182_18395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439108.1"
                     /translation="MIYARGCWHMFFEALNSESGRGEIGLASSHDAAVWDYRKIVLRE
                     SFHLSYPYVFEWDNQFYMVPESYQANSVRLYRANDFPVSWSFVGDLLSGDDFEDSCVF
                     RFNDRWWLITDHAKPPYYAGKLRLFYSDHLMGPWVEHPKSPVIDGNPHIARPAGRVVV
                     WNDRVIRFTQDCFPIYGARVRAFEITELTSTAFREQQLGASPVLQGSGMGWNESGMHH
                     IDPHPIGDGRWLACVDGWHWNSCKI"
     gene            2920..4227
                     /locus_tag="VF182_18400"
     CDS             2920..4227
                     /locus_tag="VF182_18400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6439109.1"
                     /translation="MRNFIKEHALKLLRRFKNFPLGILASEFKGFLLNAPKEMTRLER
                     RFTSRKPKTRSIGNVLLCYNNYAFFSRSEDPYLTRHTNRWESWQIAKTFVDLGYRVDI
                     INENDDRFVPTKNYSVFVGNRINFHRLTGLLNDDCIKILHLDTAHWLFNNMAEGRRLE
                     FLQRRRGFVLLPQRNLTPNLAIEHAAYATILGNEFTIGTYTYANKPLFRVPISTPVLY
                     PWQDNKDFGQVRKNFLWLGSEGFVHKGLDLVLEAFAQMPGYHLTVCGPIRTDKDFQNA
                     YWHELYETPNIHTVGWVDIGSSKFIDIAKNCVGIVFPSCSEGGGGGVITCLHAGLIPI
                     VSYEASVDVHESRGLILKESSVDEIKASVQKLSALAPQQLTAMSKRNWEFARENHTRE
                     TFAAHYSRVISEIITAESERRQLARNPIETRAVSPELVMREML"
     gene            4224..5240
                     /locus_tag="VF182_18405"
     CDS             4224..5240
                     /locus_tag="VF182_18405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX6439110.1"
                     /translation="MSKLVTIGIPIYKRLEYLPSVLKIVGSQDYPNIELIVSDNGMNG
                     AAVRDIVQRCYTRPSRFRQNPSTVGMSIHFNQIIHEASGEYFVLLADDDEISTNYVSD
                     LVDLLERYPRASVAMAVQETVDEGGTLVRRSSEQVPEILSGTEFVRAAWGTHEYNYES
                     FSTFLARTRSLSTSGGYPDFWKGSGNDDALIVKLCLDNFIVLSGRCVFRKRSYDDSYG
                     FSQSTQDLARGIKDFLQFVDRNPILQQFACDHPTEWRETRHLLVKMAWKTYYLRWADM
                     YRERLSPLQWAKAGFELPFIPEYYKAMIQTLLAAAVTPFLKAIKQHLPRAYQVYRAAR
                     PRRP"
     gene            5295..7268
                     /gene="asnB"
                     /locus_tag="VF182_18410"
     CDS             5295..7268
                     /gene="asnB"
                     /locus_tag="VF182_18410"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX6439111.1"
                     /translation="MCGIAGIAGQQDPEWIRRMNSSIVHRGPDDHGEYFSPESSVSMA
                     MRRLSIIDLQGGHQPMPNKDGSLWIVFNGEIYNAPEIRCRMEQRGTQFSTGHSDTEVL
                     LHLYEEKGFSLLQDLNGMFAFVIHDRKRNLLFGARDRMGIKPLYYCSSNGRFLFASEL
                     KALLTLPIIEREIDRQSLFHYMTLLYVPDEASIIKGVRRIPPAHSFIYDLKSRQLTLQ
                     KYWQLKFAADESPDDKEWAEVLRIELKAAVRRWTLSDVPIACSLSGGLDSSAIVGLLA
                     EAGYPRTKTYSLGFVGSEEQSWNEIDLARQVAQRWGTEHHEIILEPQDLLSDLAAMVW
                     HLDEPYGGGLPSWYVFREMSRDVKVGLTGTGGDELFGNYGKFKIYEASRMLQTSLASR
                     RYSDKPARMLAGLIAPLSTLSNSLPSSWPWIGRGHLLSQLPRIINEPFGRYYYANFHN
                     FTDDCKRTQVLRTHNGDLQDTASYLQKVYDLSEARDLRNGLAAVDFRTQLAEEFLFMT
                     DRFSMAHSLEARVPFLDHNLVETVFRIPSSIRTAAGDLKYLFKRAIGDLLPDSLLNAR
                     KRGFVIPTTLWLRRELRPLVERLLGSERLHRQGLFQSAFYNSFVLPHLEGKADFTSQV
                     WAALMFQIWHLIYIEQNQTVAPSYDWQAMAGLD"
     gene            7334..8326
                     /locus_tag="VF182_18415"
     CDS             7334..8326
                     /locus_tag="VF182_18415"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6439112.1"
                     /translation="MKLEHLDSLVSPATGLRLRLQSVQSREHGEIKEGMLVDASGDHQ
                     IPIRNFIPRFISEASYTGSFGEQWNRYRSIQIDSENQLNLSAERFYRSTGWKKDELAG
                     QHVLEAGCGAGRFTQVMLEAGAKVYALDMSSAVDACWRTNGPNSNLYLAQADIYHIPH
                     RTGFFDRIFCYGVLQHTPDPRQAFMSLVKFLRPGGKIAIDCYLKCRRLNRWTAKYLWR
                     PITTRLSSEKLFRVIEWYIPKWLPIDNKLAGIPKIGTMLVGIIPCWNYTGMLPLTPEQ
                     ITQWAILDTFDALGAKYDSPQTCAEVRSWFHEADLDNVQVGNGSNGIVGSGIKR"
     gene            8540..10408
                     /locus_tag="VF182_18420"
     CDS             8540..10408
                     /locus_tag="VF182_18420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439113.1"
                     /translation="MLANQGWNSRFISFDMLGYIDDAQRFIDHGHIPEKGILTSFASY
                     SPPGITWLFIPGTYAFTDPRIFESIGAGILYTGTLAGLFLLARPAFGLVCAYLTVTLW
                     GLSDLGLRFADTTGARGHPFFYIWMLYLAGLWIGRKDSKYLILAIVVWAVGMYCMMEI
                     APAIMIIPVVWLYFRPPLKVSALIAAGVLSGIIWLPYLRFEVTRGFLDIKSQIARQKI
                     LPTDFEKTWCNPGLLPASWVANRGLPGSSDNVIDSIPDQPSLALGLAAVFSRAKIVLS
                     KLLIANHAQASRFPGASIFLLVVTIVTMLVCGVSPSSAPPRTLHRNRRSIWLKWFGVG
                     LIFCSLAFNELTMAFLSPDGALQPSTVLIIRIFQLVCFTCALILLTVRETIVAVISRL
                     WQTSSLLPSEASNSIGRKFVAIALAVPWLILLLLTEEASPQRFWWLWPLQIMTLAGAV
                     TYFPNRFNWPRFWVWTGSLVLAFMLLSTELLASRLTGWARDGWSGKESDEIRVIDLVA
                     RLIESERTETAAIGYSILVSRFMATFNAADLRYKVGADFNLFFKSRHGIRNTNDCPEG
                     ISPNDNYRIAQSYQEASAGQGYITAPVDTRFRTVAEVGSYRILKRVRAGKIPNNDV"
     gene            10573..>10939
                     /locus_tag="VF182_18425"
     CDS             10573..>10939
                     /locus_tag="VF182_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439114.1"
                     /translation="MAVGTKTSDDPDVILIPNESCTNRWTRMWTGMAMRLEEKAPLGR
                     GFYRMASLARLVSNPLRWIDIWLGHEDFHFPGTWRLLDLVDRPQVVHCFNLHGRYFDL
                     RALSSISRKVPVVIDLRDAW"
BASE COUNT         2523 a         2767 c         2906 g         2743 t
ORIGIN      
        1 gaacatcgct gagggacttc ctacgggcca gtgatctaag tcggatcgat gatgacgttg
       61 tcaagctcta taccgatgga actgaaaaca tgggcgattt agtttaccaa cccactagac
      121 cgaccaccgc cgctgaataa attactgggc tgctcaaaat catgtctcat gatgaagtca
      181 tcggactgat tccagcggga ggacttggca cgcgcttagc gcccttgcct tgtagcaagg
      241 agctatatcc catcggcttc cgggaatcgc ctgggcaagg tggttggcga ccaaaagtag
      301 tttgtcatta ccttctggaa aacatgcggc gggccggtat caccaaagca tacatcgttc
      361 tcagagacgg caagtgggac attcctgcct atctcaagag cggcgccata gtcgatatgc
      421 acctttctta cctggtgctc gctcttccct tcggcgcgcc gtttacacta gatgaagctt
      481 atccattcat tcgacaatcc gtagtggctt tcggttttcc tgacatgatt tttcaacctg
      541 aagacgcctt ttcacaatta cgttcgcaac aagcatccag tgatgccagt gttctactag
      601 gcctctttcc ttcaatggaa ccggacaagg tggacatggt cgacatcgag ccggacggtc
      661 gcgtagtgaa aatcgacatt aaacctgcca gaaccgacct aacttatacg tggggcatcg
      721 cattatggcg tccgattttt agcgatttta tgcatgagta cgttagcgaa aggaaatcgg
      781 acgctgctac tcaaccagag atcaccgttg gaaatgtcat tcaagcagcc attgaggccg
      841 gactgaaagt agatgggtca ccggtatcaa cggaaccgta tctggatatc ggcacatcgg
      901 cagggttgat acttgcgata cagaagcact gctcatctag tccacgcgcg tcaggtagga
      961 attcaatttg accaccaact tcgtcgaatc gttttcccaa tacgtgaatc gcaaagtact
     1021 gctaactggt gccacaggat tcatcggagc tcctttgtgc cggcgcctga tcgaacatgg
     1081 cgcagaagtt tatgggatct ctcgaagcgc ccaagagccc gcggcaagcg gaatgcgctg
     1141 gttccaggcc gggcccgccg atccatgtaa gatccgccag attctcagtg agacaaaacc
     1201 agacatcatt tttcatctgg ccggcatggc gacagccgct acagaactcg accttgtcgt
     1261 gcctacgttt gacagtcttc tcgcaagtac cgtgtatcta cttaccagcg cggcggagct
     1321 tggatgcggc cgaatcgtca ttccagggtc tctgacggag ccgaaagaag atgacgttga
     1381 tgctactcca ggctccccgt atgccgccgc aaaatgggcc ggttcagcgt atagccggat
     1441 gttttttaaa ctctatggca ctccagtggt gattgctcgg ccgtttatga cgtatggccc
     1501 gggacagccc cggagcaagt tagtacccta tgtgattctt gctgcgctgc gccgagaagc
     1561 gcctaaaatt tcgagcgggc agtggcaagc tgattggatt tatatcgatg atgttgtcga
     1621 tggattcatg gcggccggtt gcactccagg aatctgtggc aagacaattg atcttggttc
     1681 gggtgcactc acctccgttc gtgccgtagt gcaaaaaata atcgatctga cagatcagga
     1741 agtccaccca atatttggtg ctcagcgcga tcgacgattt gaggaagttc gtgttgctga
     1801 taccgagttc gcctatgcaa cactgggttg gaagtcaacc atctcgctta ccgaaggact
     1861 cgcgcgaacc gtcgattggc tacgagagca cgtaaaccaa aaccggacat catagcgctg
     1921 gcaccactct gttgatcgct cgattggccc agcagagaac cacctttgtc cacatgcgaa
     1981 tatgaagagc ctcttctctc gggccatccg tagactgctt ggcaccatgc ccgccccaga
     2041 gccggaccca gtcaagacac agatatggtc gattgggatt tataccggag attctcccta
     2101 ttcagtccgg ccgactaata gcgctaacaa tcctgttctc acccgcgctt cggtttccga
     2161 tgtgcaagcc cgatttgtcg cggatccgtt tatgatttat gcccggggat gttggcatat
     2221 gttcttcgag gcgctcaatt cggaatcggg tcgtggagag ataggacttg catccagcca
     2281 cgacgctgct gtgtgggatt accgcaaaat cgttctgaga gaatcattcc atctctctta
     2341 tccgtatgtg ttcgagtggg ataatcagtt ttatatggtt ccggaaagtt atcaggcaaa
     2401 ttcagtcagg ctttaccgag caaatgattt tcccgtgtcc tggagtttcg tgggcgatct
     2461 cctcagcggc gatgatttcg aagatagctg tgtgttccgc ttcaatgatc gatggtggct
     2521 tatcaccgac catgcaaagc cgccctatta tgccggcaag ctgcggctat tttattctga
     2581 ccatctgatg gggccatggg tcgagcatcc gaagagtcct gtcattgacg gcaatcccca
     2641 catcgcccgg ccggctggca gggtcgtagt atggaacgat agagtcatcc gctttaccca
     2701 agactgtttt cccatatacg gcgcccgggt ccgcgccttt gagattacgg agttgacttc
     2761 cacggctttt cgggagcagc agctgggcgc atccccggta cttcagggca gcggtatggg
     2821 atggaacgaa tctggaatgc atcatattga tcctcatcca atcggcgacg gccgctggct
     2881 ggcgtgcgtg gatggatggc actggaactc ctgcaaaata tgagaaattt cataaaagaa
     2941 catgcgctaa agctgcttcg gcgcttcaag aacttcccac taggtatttt ggcaagtgaa
     3001 ttcaaaggat tcctgctgaa tgcgcctaag gagatgacca ggttggagcg cagattcacc
     3061 tcacgaaagc caaagacaag atcaatcgga aacgttttgc tctgctataa caactatgcc
     3121 ttcttctcga ggagcgagga cccttatctt acaaggcaca ccaaccgatg ggaatcgtgg
     3181 cagatcgcta aaaccttcgt cgatctgggc tatcgtgtcg acatcattaa cgaaaacgac
     3241 gatcgtttcg tgcctaccaa aaattactcg gtctttgtag gtaatcgcat taactttcat
     3301 cggcttactg gtctgctcaa tgacgattgc attaaaattc tccatctcga tacagcacac
     3361 tggctcttta ataacatggc cgaaggccgg cgcctcgagt ttcttcaacg gaggcgcggt
     3421 tttgttttgc tcccccaaag gaatctcact cccaatttag ctatcgagca tgccgcatac
     3481 gcgacgattc tgggtaacga gtttaccatc ggcacgtaca cgtacgccaa caagccgctt
     3541 ttccgtgtgc cgatttcgac tccggtccta tacccctggc aggacaacaa agatttcggc
     3601 caggttcgta agaacttttt atggttgggc agcgaaggct ttgttcacaa agggttggat
     3661 ctggttttgg aagccttcgc acagatgccg ggctatcatt taacagtgtg cggcccgatc
     3721 cgtacggata aagatttcca aaacgcgtat tggcacgagc tatatgagac accgaacatc
     3781 cataccgtcg gttgggtgga tatcggcagt tctaagttta tcgacatcgc taagaactgt
     3841 gtcgggattg ttttcccgtc atgctcggaa ggcggaggcg ggggcgtcat aacctgcttg
     3901 catgccggtt tgatcccgat cgtgagttac gaggcaagcg tggacgtcca tgagagccgc
     3961 ggcttgattc tcaaggaaag ttcggttgac gaaatcaaag catcggtgca aaaattatcg
     4021 gctttagcgc cgcaacagct tactgccatg tcgaaaagaa actgggagtt tgccagagaa
     4081 aaccatacga gggagacgtt tgcggcacat tactcgagag ttatatcgga aataatcacc
     4141 gccgaaagtg agagacgaca actcgccaga aacccgatcg agaccagggc tgtttcccct
     4201 gagctggtga tgcgagagat gctatgagca agctggtaac gatcggtatt ccaatctaca
     4261 agcggttaga gtacttgccc agcgttctga agattgtcgg ctcgcaggat tatccgaata
     4321 ttgaactgat tgtctccgat aatggcatga acggagcagc tgttagggac atcgtccagc
     4381 gatgctatac ccgcccgagt aggtttcgcc aaaatccgtc cactgtcggc atgtcgattc
     4441 attttaacca gattatccat gaggcctccg gagagtattt tgttttgctc gccgatgatg
     4501 acgaaatcag cacaaattat gtttctgacc tggtcgatct cctcgagcgt tatcctcgag
     4561 cctcggtcgc gatggcggta caggagacgg tcgatgaggg gggaaccctg gtcagaagat
     4621 ccagcgaaca agttcctgaa atattatcgg gaactgagtt tgtccgcgcc gcatggggaa
     4681 ctcatgagta taactatgaa tcgttctcaa ctttcctcgc aagaacgaga agtttgagca
     4741 cctccggcgg atatccggat ttttggaaag gcagtggcaa cgacgatgcg ctgattgtca
     4801 agctgtgtct ggataatttt atcgtgctaa gcgggcgctg tgtcttccgc aagcgctctt
     4861 acgatgacag ctacggcttc tctcaaagta cccaggatct cgccagggga atcaaagatt
     4921 ttctgcagtt cgtcgatcgt aatccgatcc ttcagcagtt tgcgtgcgat catccgaccg
     4981 agtggcgtga aaccaggcac ctcttggtca agatggcatg gaagacttat tatctgcgat
     5041 gggccgatat gtaccgggag agactttcgc cgttgcaatg ggcgaaagca ggattcgagc
     5101 tgccctttat tccagaatat tacaaggcaa tgatccaaac tttgctcgca gcagcagtaa
     5161 ccccattttt gaaggctatc aaacaacatc ttccccgcgc atatcaggtt tatcgcgccg
     5221 ctaggccaag acgcccgtag aagaacgcgg caatcaatca gtctctactc atcgaaagca
     5281 gcgggtccaa gaacatgtgc ggaatcgcag gcatcgcagg gcaacaagat cccgaatgga
     5341 tccgtcgaat gaattcgtca attgttcatc gcgggcccga cgatcacggc gaatacttca
     5401 gcccggagag ctcggtatcg atggcgatgc ggcgcttgag tatcatcgat cttcaaggcg
     5461 gtcatcagcc gatgccgaac aaagacggtt cgctttggat tgtgttcaat ggcgagatct
     5521 acaacgcccc ggaaattcga tgccgcatgg aacagcgggg cacgcagttt tcaaccggcc
     5581 attccgacac cgaagtattg ctccatcttt atgaggagaa aggcttctcg ttgctgcagg
     5641 atctcaatgg catgttcgct tttgttatcc atgatcgaaa acgaaatctg ttgtttggcg
     5701 cgcgtgatcg gatgggcatc aagccgcttt actattgctc ctccaatggc cggtttctct
     5761 tcgcttcgga attaaaagct ttgctcacac tgccaatcat cgaacgagag atcgatcgac
     5821 agagtttgtt tcactacatg acgctcctct acgtgccgga tgaagcctcc atcataaaag
     5881 gggtgcgccg gattccgccg gcgcacagct tcatctacga tttgaaaagt cgccagctga
     5941 cgttgcaaaa atattggcag ttgaaatttg ctgccgacga atctcccgat gataaggaat
     6001 gggccgaagt tctacgcatc gagttaaagg ccgcggttcg tcgttggaca ctgagcgacg
     6061 tgccgattgc ttgttctcta tcgggtgggc tcgattcgtc tgccatcgtc ggtttgctgg
     6121 cggaggctgg gtacccgagg accaagactt attcgctcgg atttgtcggt tcggaagagc
     6181 agtcgtggaa tgagatcgat ctcgcccgtc aggtagccca acgctggggg acggagcatc
     6241 atgagatcat actcgaaccg caggatttgt tgtccgacct ggcggctatg gtctggcacc
     6301 tggatgaacc ttacggcggc ggattgccgt cctggtatgt gttcagggag atgagccgtg
     6361 acgttaaggt cggactcacc ggaacgggag gcgacgagct cttcggaaac tacggtaagt
     6421 ttaagattta cgaagccagc agaatgttgc aaacatcgct ggcatcgcgg cggtactccg
     6481 acaagcctgc ccgtatgttg gccggcttga tcgcgccgct ttccacactg agcaactctc
     6541 ttccatcgtc ctggccctgg attgggcgcg gtcacctact gtcgcagctg ccgcgcatta
     6601 tcaacgagcc gttcgggcgc tactattatg cgaatttcca caacttcacg gacgactgca
     6661 agcgcaccca ggtgctgcga acccataacg gagatctaca ggacactgcc tcttatctac
     6721 agaaggttta tgatcttagt gaagctcgcg atctacggaa tgggttggct gctgtggatt
     6781 ttcgcacaca gctcgcggaa gaattccttt ttatgacgga tcgcttttcg atggcgcata
     6841 gcctggaagc tcgagtgcct tttctcgatc ataatttggt cgaaacagta tttcggattc
     6901 cgtcctcaat ccgtaccgcg gcgggcgatc taaagtatct tttcaagcgt gccattgggg
     6961 atctcctgcc ggattcattg ctaaacgctc gaaagcgcgg ctttgtgatt cctacaacac
     7021 tctggctgag acgcgagctt cgtccgttgg tcgaacgctt gctgggttct gagcgtttac
     7081 atcgccaggg cttgtttcag agtgcctttt acaattcatt tgtgctgcct catctggagg
     7141 gcaaggcgga ttttacctcg caggtttggg cggctctgat gtttcagatt tggcatctga
     7201 tttacatcga gcagaaccag acggtggctc cctcgtacga ctggcaagcc atggcaggct
     7261 tggactaatt gagtacagcg gcaattcgcg cctgatcgat agctcatgaa atttcatttg
     7321 ctgtggtaac gagatgaaat tggaacacct tgacagttta gtttcacccg cgaccggact
     7381 caggctgcgt ttgcaatcag ttcaatcgcg ggagcacggc gaaatcaaag aggggatgct
     7441 agttgatgcc tcgggagatc atcagattcc aattcgcaat ttcatccccc gtttcatctc
     7501 ggaagcgtct tatacgggca gtttcggaga gcaatggaat cgctatcgct cgatccagat
     7561 agatagcgag aaccagctta acttaagcgc tgagaggttc taccgttcga cgggttggaa
     7621 gaaagacgag ctcgcagggc agcatgtttt agaagccggc tgcggtgccg gaagatttac
     7681 gcaagtgatg ctcgaggcgg gagccaaggt ctacgcttta gacatgagct cggcggtcga
     7741 tgcctgctgg cgcaccaatg ggccaaacag taacctttac ctggcacagg ccgatatcta
     7801 tcacatcccg catcgcacgg ggttttttga ccgaatattc tgttacggtg ttctccagca
     7861 cacgcccgat ccacggcaag ctttcatgtc cctggtaaag ttcttgcggc ctgggggaaa
     7921 aatcgcaatc gattgctacc ttaaatgccg gcgtctcaac cgctggacgg ccaagtacct
     7981 ttggcgccca attaccaccc ggctttcctc ggaaaaactt tttagagtca tcgaatggta
     8041 tatcccgaaa tggcttccga tagacaacaa attggccggc atccctaaaa tcggtaccat
     8101 gctcgtgggg atcataccgt gctggaacta tactggcatg cttcccttga cgcctgaaca
     8161 aattacccag tgggcgatac tcgacacgtt tgacgctttg ggagcaaagt atgacagccc
     8221 gcaaacttgt gccgaagtcc gttcatggtt ccatgaggct gacctggaca atgtgcaagt
     8281 cggaaacggt tccaacggga tcgtcggcag cgggatcaag cgatgaattg caaatttgat
     8341 tgaacgtatt cgcaagtagg taaattaacc cttctcctca cagttgcgtc ctagtcatta
     8401 ttggctatcc caagtaggcc gacctcagat cagtacggtt gcagtgtcat tacagggtat
     8461 gacttgtcgc caaatacagc cgggtgcccg cctcacgtac cattggctgt tcgtattagc
     8521 tatcgcggcg agcgggatct tgctggccaa tcaaggctgg aattcccgat tcatcagctt
     8581 cgacatgctg ggctacatcg atgatgctca gaggttcatc gatcacgggc acattcctga
     8641 gaagggaata ttgacgagtt ttgcctccta ctcaccgcca ggcataacct ggctattcat
     8701 ccccggcacg tatgccttta ccgatccaag gatctttgaa tcgatcggcg ccggcatcct
     8761 ctacacaggg acgctggccg ggctcttctt gctggcgcgg ccggcgtttg gtcttgtgtg
     8821 cgcgtacctg acggtgacgc tctgggggct gtccgatctt ggattgcgct ttgcggatac
     8881 taccggagca aggggtcacc cgttcttcta catctggatg ctctatttgg ccgggctatg
     8941 gattggtaga aaagactcta agtacttgat tctggctatc gtggtttggg ctgtgggcat
     9001 gtactgcatg atggaaatcg ccccagcgat aatgattata ccggtcgttt ggctctattt
     9061 tcggccgcct ttgaaagttt ccgctttaat tgctgccgga gttctaagcg ggattatttg
     9121 gttgccttac ttgcggtttg aagtcacgcg ggggttcttg gatatcaagt cacaaatagc
     9181 gcgtcagaag attcttccaa cggattttga aaaaacttgg tgtaatccgg gattgcttcc
     9241 ggctagttgg gtagccaacc ggggattacc cggttcaagt gacaatgtga tagattcaat
     9301 accagatcaa ccgagtttag ctttggggtt ggcggctgtc ttctccagag cgaagattgt
     9361 cttgagcaaa ttgctaatag cgaaccatgc tcaggcatcc aggtttcccg gcgccagcat
     9421 ctttcttctg gtagtcacca ttgtgaccat gctcgtttgc ggcgtctcac cgtcgagcgc
     9481 gccaccgaga acacttcatc gcaatcgacg atcgatttgg ttaaaatggt tcggagttgg
     9541 ccttattttt tgttcccttg cgttcaatga gcttacgatg gcgttcctga gtcccgacgg
     9601 cgcactacaa ccatcaaccg tgttaatcat tcgcatcttt cagctcgtct gtttcacctg
     9661 cgccctcatc ctgttgaccg tgagagagac catagttgcg gtcatctcac ggctatggca
     9721 aacctcaagt ctcctgccgt cagaagcatc gaatagcatc ggtcgaaaat tcgtcgcaat
     9781 tgccttggcg gtcccttggc tgatattgct tttacttaca gaggaagcta gcccgcaaag
     9841 attttggtgg ttatggccgc tgcaaattat gactttagct ggagccgtca cttactttcc
     9901 gaatcgcttc aactggcccc ggttttgggt ttggacaggt tctctcgtat tggcgtttat
     9961 gcttctcagc accgagctct tggcttctcg actgacagga tgggctcgcg acggttggtc
    10021 gggaaaagaa tcggatgaga tcagagttat tgatctggtc gctaggctga ttgagtcaga
    10081 gcgaacagag accgccgcca taggttattc aatattagtt tcccgattca tggctacgtt
    10141 taatgcagca gatcttcgat ataaggtcgg cgcggatttc aacctatttt tcaagagccg
    10201 acacggaatt cggaatacca acgattgtcc cgagggaatt tcaccgaatg acaactatag
    10261 aatagctcaa tcatatcaag aggcgagtgc cggtcaagga tacattactg ctcctgtgga
    10321 tacgcgcttt cgcacggtag cggaagtggg ttcttatagg attttgaaac gggttagagc
    10381 agggaagata ccaaacaacg acgtttgacg acttgtgcga tcggcggaca atatctgatg
    10441 aaaggtcaaa gtcggaacga gcaagatcga gtgcgtattc tattagttag cacgtccgac
    10501 gtcggaggcg gtgccgagcg atcggcgtgg agcttattaa aagcatatac tgaggccggg
    10561 cataggagct ggatggccgt cggcacgaaa actagcgatg atccggatgt tatcttaatt
    10621 ccaaacgaat cgtgcaccaa ccgatggacc aggatgtgga ccggcatggc gatgcggcta
    10681 gaggagaaag caccgctggg tcggggattc taccgcatgg cttctctcgc gcgcttggtc
    10741 agcaacccgc tccgctggat cgacatctgg ctgggacatg aggactttca ttttcccggc
    10801 acctggcgct tactcgatct ggttgatcgt ccgcaggttg ttcactgctt caatttgcac
    10861 ggacgctatt tcgatctgcg ggcacttagt tcgatcagcc ggaaggtacc tgttgttatt
    10921 gacttacgcg acgcttggc
//
LOCUS       DASUVS010000350        48753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1897649, whole genome shotgun sequence.
ACCESSION   DASUVS010000350 DASUVS010000000
VERSION     DASUVS010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 48753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48753
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897649"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VF182_18430"
     CDS             complement(<1..753)
                     /locus_tag="VF182_18430"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEX6439115.1"
                     /translation="MNVIHKIIFWSHLIAGVVAGTVIFLMSATGVILMYEHQIVEYAE
                     RNVREVPPRIGTARLSLDEVIAKTRAQNLNAQPTAVVLRNQPTAPVAVGFGREGAVYV
                     DLYTGAVLGQESKLQRWFHDVTDWHRWLGQDGEGRATGRAITGACNLAFFWLAITGVY
                     LWWPRSWRWRGLKSSLLFNPRLGGKARDWNWHNVIGFWSSSVLVVLTMSAAVMSYPWA
                     NDLLYTLTGSESPPRSQGSVPAGQATRRSLSEE"
     gene            complement(789..1469)
                     /locus_tag="VF182_18435"
     CDS             complement(789..1469)
                     /locus_tag="VF182_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEX6439116.1"
                     /translation="MIVQVPNVLKNQQLTRCRQVMERASWVDGRVTAGHQSAQVKDNR
                     QLPENSAEARELGDMIVAALERNPLFITATLPLRVFPPLFNRYEGGESFGAHLDNSIR
                     QIPGTPLRVRTDISATLFLSAPEEYDGGELVIDDVYGAHAVKLPAGDMVLYPASSLHH
                     VNPVTRGARLASFFWVQSMVRDDGNRTLLFDLDMAINKVNQALPNHGAVVELTNCYHN
                     LMRRFAEV"
     gene            complement(1483..3633)
                     /locus_tag="VF182_18440"
     CDS             complement(1483..3633)
                     /locus_tag="VF182_18440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEX6439117.1"
                     /translation="MSRQQRRALEQDQRKTALRKICLAAGEGVGKALALSFALGSNAV
                     AAEPKKEETFQLPPVVVQDQNSPYVVPQSSLSKFPVPLKDTPQSITIVPEKLIEEQAG
                     TTLRDALRNVPGIAVAAGEGGGSQGDSFTLRGFNARNDMFIDGVRDAGSYFRDSFNFE
                     SVEVLKGPSSTFFGRGSTGGIINQVSKVPRLDPSWEGMFSGGSALFLRGTIDVNQPLP
                     KDALPNAALRLNLMAQRADVVDRDKIEGNRLGFAPSISFGLGTPTQLTASYLLQHEDN
                     IPDYGFPYADGKPLRIDRDTFYGLNKEDHEETWVNVGTLRFDHRFNDIFNLRNTLRYS
                     HVDRDSAVTNATAVLPNTLNRSRPQRDTQESILGNQTDLIAKFDTFGFGHTATTGVEV
                     SRETFDLSRWNSAGPDTTIENPDSHQLPAPKALAADSDTRATGFGIYAADQIKLNSYF
                     DILGGARWDYFSAHIKDDFADDTRNQIDQMWSYRGGLVFHPTASQSYYFSYGTSFNPS
                     AEGIALTPTTNGTPPEKNRILEAGAKLEFFEGALNFQTAAFRIEKTNARTINPDDPTG
                     PNIVTGEQRSQGFEVGLTGRLVPGLNVFGGYTYLDTEILEDNTAANVGNEIANVPQHS
                     ATLWTTYDFFEKWQIGGGPTYVGSRFSNNANTNRIPGFVRWDSTIAYQITRNIQLRIN
                     AINLTNQLYFDSVAGSKAVPGAGRTFIASASFKF"
     gene            complement(3713..3904)
                     /locus_tag="VF182_18445"
     CDS             complement(3713..3904)
                     /locus_tag="VF182_18445"
                     /inference="COORDINATES: protein motif:HMM:NF022103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HEX6439118.1"
                     /translation="MGESDLKVAPEQAIVTRVAVLTDKKPRIQSDSLFQGKTEVVIVH
                     QREEYNLRITRNGKLILTK"
     gene            complement(3912..4772)
                     /locus_tag="VF182_18450"
     CDS             complement(3912..4772)
                     /locus_tag="VF182_18450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX6439119.1"
                     /translation="MTVDRWLLSSLTASSLIHLALISLAALMVHTRPAKPIKVPIELI
                     EISPAERPKIQLAPAPEPPSMPRIKPKPQEITAPKLTSKPKIIETKPVPVPGKPPEDI
                     KQPEPMVKSPPPAPAALPANPGPSEGGWNAADSPGQAKGSAAGAGNLFDNGEVGVVGG
                     TGIEGGGGGEGISGLGKRTEGDGTGGGGANSGEALSGTARPIGGYQVKPRYPASARRA
                     GAQGVTRLRVHVLESGRVGEVMVDESAGFRDLDLAAMDAVKKWRFEPARRGKEPVSVW
                     VMLPVKFELN"
     gene            complement(4762..5181)
                     /locus_tag="VF182_18455"
     CDS             complement(4762..5181)
                     /locus_tag="VF182_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017314425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX6439120.1"
                     /translation="MRIQRRSIKKARIEIIPMIDTIFFLLVFFMISTLSMAQYRGMPV
                     NLPKAASGQQAPAESAAITIDSQGRLFLDKQPIEKTTLGDLLRQQLAQNADLLVVINA
                     DDGVEHGRVVEVMDIARTANIARMAIAVKPKQESNDR"
     gene            complement(5181..5789)
                     /locus_tag="VF182_18460"
     CDS             complement(5181..5789)
                     /locus_tag="VF182_18460"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX6439121.1"
                     /translation="MSDVVAIIIKGGIVMVPLLACSLISLALSIERFFFWAKIKSPQT
                     VQHMFGLAEAGEFDRAIKLGKESQHPIARVLAAGLTHRIPGPSKAMEAAAQAQIPVLK
                     KRLGALDTIITLAPLLGLLGTVVGMIGSFDIMSEAGMGQPHAVTGGVAEALIATATGL
                     LIAILTLIPYNYFSSRVEKEMDAMEHYSSSLELLLHEDAGKH"
     gene            complement(6341..8317)
                     /locus_tag="VF182_18465"
     CDS             complement(6341..8317)
                     /locus_tag="VF182_18465"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c/FTR1 family iron permease"
                     /protein_id="HEX6439122.1"
                     /translation="MSAPHQNSGFMRLRQMTLVLLLVVFSASTLWAADEANRIVALID
                     YIGGDYRNAVQNGKVVNDDEYQEMSEFSARSLELFRQLKSAEGDKAVIEKDLTVISAH
                     IKNKSGEDAVPELAQQIKDRLIKTYNIATYPKLLPNYVIAKTLYLENCAQCHGETGKG
                     DGPGRESMNPRTPSPANFTNPDFMTGLSPFKVYNTTTFGVDNTAMASFAPLPDEQRWQ
                     LAFYVMALRFSAEDAEAGEAWLKGKAIPGDLTSTETLATSSDEDLSGRIKKHFPDAPG
                     EKVLAYFRRGLLEKKTSDPLLIARTLVGEATNLYAGGDKEKSYQKAIEAYLDGFEMAE
                     PALFAKDAALGKTLESQFMEFRNAIRQGVPLADLQKKLQTIEANLDLAAQKLTAAGGG
                     GFPGYYAFLNSALIILREGLEAALILAAIVAMLKVMGASDAIRYIHLGWILALFGGVL
                     TWLAAQTILTFSGQHRESMEGFISIFAAVALFYVGYWLHTRSEAKRWQAFIQDRVQNA
                     LSSKRILGLVGISFFVVYREAFEVVLFYQALWLQNEASRQSIVGGFVSGVIVLLAATF
                     AIFKLGVCIPLKYFFGITGTLLYIMAFIFAGTGINQLQAAGWVPATPLEFPPAVPLLG
                     IYPTIETLTVQFLLLCAFIVTLFSMGHERKKAVA"
     gene            complement(8314..9990)
                     /locus_tag="VF182_18470"
     CDS             complement(8314..9990)
                     /locus_tag="VF182_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6439123.1"
                     /translation="MTTAAAVCYRFVNGIRGVKLGWLGWSSITVACLVLAPIAAVVSN
                     LFLPSKATWSHLLATVLPEYIWNTLQLVLLVAIGVVLFGVTTAWLVSAYRFPGQRALE
                     WALVLPLATPAYVMAYVYTDWLQAAGPVQSVFRELTGLKVREYWFPEIRSLPGAAAML
                     TFALYPYVYLLARTAFLEQSRTTMEVARLAGYGDWGRFLGVALPLARTGIVAGTALAL
                     MEALADFGTVSYFAVNTFTAGIYRAWLSLGDPVAAGQLATCLLVFVLLMLSWEQRHRG
                     GARYAAKRIPMQPQLLYGPAAAAASIACALPVTFGFFLPAALLIRLALRDPEAQFGAR
                     LYGLVLNTFTLAGVAAFVAVAVALLLAYAGRTVKHPLVHIANRFAVLGYALPGAVIAV
                     GILLPLGKLDNTLAGWVQEQLGIKAGLLLTGSMVALIYAYVVRFLAVAFHTAEAGLTR
                     VTLSMDEAARSLGYSPVHTLWRVHVPIISGSLATAVLLVFVDVMKELPATFAMRPFNF
                     DTLAVEAYNLAKDERIAEAAVPSLVMVTIALLPLVFLSRQIARSRREEPT"
     gene            complement(10018..11073)
                     /locus_tag="VF182_18475"
     CDS             complement(10018..11073)
                     /locus_tag="VF182_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6439124.1"
                     /translation="MHAPAGNRRKIFASILSIATCVLTSSIGEARAQEKVLNLYTARH
                     YATDEAFYTGYTKATGIKINRIEGGEDALFERIKAEGANSPADVFLTVDAARLWRAEQ
                     AGIFAPVKSSIIDKRIPANASGADNKWFGFSARARVIAYDKSKIKPAELARYEDLANA
                     KWKGQICVRPSSHPYNLSLISSLISHLGEEKATEWARGVTANLARPPRGGDTDQLKAV
                     EVGECSIAISNHYYYVRLMRSDKPEERALVEKVGLIWPNQADRGTHLNISGGGLLQTA
                     PNRNAGIKFLEYLASDAAQTLFANGNNEWPIVKGVKLDNAALESLGQFKTDSLPLANL
                     GKSQAAAQRIADKVGWK"
     gene            complement(11266..12321)
                     /locus_tag="VF182_18480"
     CDS             complement(11266..12321)
                     /locus_tag="VF182_18480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6439125.1"
                     /translation="MVIIEVKEIGKRYKKTVAVKSCDLQVASGKILALLGPSGSGKTT
                     LLRLIAGFERPDEGLIVINGRTVVDVEKSIWVSPEDRGVGMVFQDYALFPHLTVAQNV
                     RFGLRNSSKTERRRRVEELLRLTELTNCADRYTHQLSGGQQQRVALARALAPRPGVVL
                     LDEPFNGLDPDLRPQMRREVARILRQLGTAAILVTHDQEEALGMADHVAVIRNGELQQ
                     VGTPEDIYHSPTTPFVANFVGQADFIPGQVLGSRIHTELGVFPCSVELADGPAKLMVR
                     HEAVNSKTAGVLATVEEREFLGGEILYRLRLPSGAMIHLEQRQPVHWPLGHQVAVELH
                     LLNVVVFPEPGSSPAPG"
     gene            12563..13228
                     /locus_tag="VF182_18485"
     CDS             12563..13228
                     /locus_tag="VF182_18485"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX6439126.1"
                     /translation="MEAHHVTHALEDYLKAIYQLSEEDRPVIAARLATETGVSPSTIF
                     ATLRRLAKEGYIKIDRRKEIHLTEAGKQVAENIVRRHFLTERFLTDLLGLDWVKAHQE
                     AHRLEHAISQEVEERLAKLLSHPTTCPHGNPIPGASSTPGPKTMPLSEAADGQDVELD
                     YITEGGERDVRLLGYLQAHGLRPGAKVHIVDVAPSLGMMTLTVNGDEFSLGIEAAKKI
                     RVH"
     gene            complement(13343..13591)
                     /locus_tag="VF182_18490"
     CDS             complement(13343..13591)
                     /locus_tag="VF182_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439127.1"
                     /translation="MAQIKHKQQLRLQELVGAAKQMNIEVRTEKLLREVGYKPRSGRC
                     RINGQELILIDRDAPLSEQIDFLAAILAEEESQPSART"
     gene            13782..14618
                     /locus_tag="VF182_18495"
     CDS             13782..14618
                     /locus_tag="VF182_18495"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6439128.1"
                     /translation="MIKNILLPLDGSEHSQAAFDYGLWMTEKFDGTLFGQHVIDTISI
                     EGTFFHDISGSMGFEPYLDFSTKMREALEERGKLILEEFAQRCRKSGIKHQTVLDMGL
                     IANEICERSKIADLVVIGHRGINEDFTTGLLGTTAESITRKCPRPVFVSTKKFKPIER
                     PLLAYDGSQRACAAMESAAEFCTVLSLPLSVLTIAKEDQLGEGILHQAKSYLNSYSID
                     VHYEKGRGYPEQKIIDHLVNFNHDLLFIGAYGHRRIIEMVLGSTTEFVLRNSSCPVFL
                     TR"
     gene            14652..15458
                     /locus_tag="VF182_18500"
     CDS             14652..15458
                     /locus_tag="VF182_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX6439129.1"
                     /translation="MKFTAAAVQMLASDDKTANLAEAARWIRQSAAAGAKLVALPEVF
                     IWRGDKRSERAAAEPIPGPASKAMAILARELGIYLLAGSILEEIPGSAKAFNTSLLFD
                     PSGKLIATYRKIHLFDVELANKVSARESETRAAGDTVVIAETELCSMGLSVCYDLRFA
                     ELYRQLAQRQADLIFVPSAFTAYTGKAHWEPLLRARAIENQVYVIAPNQFGKNPRSFE
                     THGHSMIVDPWGEIISVIADGPGFISAEVDLDYLSQVRRELPCLTHRKLS"
     gene            16037..17254
                     /locus_tag="VF182_18505"
     CDS             16037..17254
                     /locus_tag="VF182_18505"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX6439130.1"
                     /translation="MKKGLLGLILTAAIAALELPAALAQDKPLGPGWLSLDNNVGLLD
                     RNIAEGKSAIEGALGIGISGFLDTSYQWSSNHPKNPRNISGRYFDKDYNKLVFNYFHV
                     AVDKPEKDWGVGFRLSGDFGRGGELLREATLWGKSFHDEPSAEVREAYLTTTIPIGAG
                     ISVKGGIFVTTLGAEILPNAGAYNDNISRSFLFNYAIPLRHAGALFSYPILKTLSVNA
                     GVVTGWDNPRDNNRQPSFVGGLGFTPTEQFALASNVIYGPEKFSNTGDQRFTMSHVAT
                     YKPSDPLALILEYTYGREENASLGGTRDAVWQGIAGIASYGWTDRFTTALRGEFFNDR
                     DGARLGGNFSGTHANNSIAEATLTGSYKFTRMLLGRVEVRQDWSDEAFFKKGATSADK
                     NQTTVAGQLIYTF"
     gene            17702..17974
                     /locus_tag="VF182_18510"
     CDS             17702..17974
                     /locus_tag="VF182_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439131.1"
                     /translation="MIDKETELLGSAGAEGMLIYNYRLVSYSVSQLDAARFAAGAKER
                     VSQGACSRPETRDDLLKQGVTLRYSYFDKDRQHIATIDVLPADCGF"
     gene            complement(18035..19051)
                     /locus_tag="VF182_18515"
     CDS             complement(18035..19051)
                     /locus_tag="VF182_18515"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6439132.1"
                     /translation="MVRSPVLLIMGLLTLSYAPAHAQDSFYKGKTVRIVVGAAAGGGY
                     DTYSRTIARHMGKHIPGNPTFIVENMPGAGFLISANHMYNIAKPDGLTIGHFIGGLFL
                     QQLLGKEGIQFDARKFGYVGAPAQDNYVLAVSKSTGFTTIEQWLSSKTVIKFGGVGVG
                     SATDDIPKVLKETIGLPIQVVSGYSGTSTVKLAFNSGEVQGICNAWESLKATWRPELD
                     SGALNIVLQNIPKSHPDLPKIPLAINFAKTDEAKKLINALVHSVGPTARPYVLPPGTP
                     KDRVQVLVRAFMETMKDAEFIADATKAKLDINPLDGAELERNVQAVFDLDAALVQQAK
                     EIMK"
     gene            complement(19081..20103)
                     /locus_tag="VF182_18520"
     CDS             complement(19081..20103)
                     /locus_tag="VF182_18520"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6439133.1"
                     /translation="MFKFMGKGLSALVVSLLLAVPAGAAAPYYEGKAIRLIVGFSAGG
                     GFDTYSRVIGRHIGRHIPGNPSIVVENMTGAASLVAANHVYKVAKPDGLTILNFHGNQ
                     VINQIIGKPGIEFDARKFEYIGIPTQDNVACAFTKASGITSIDALRNAKTPVKVGGVA
                     PGDTTYNTAKLLIAAVKLPIQLVAGYKGTAEVRLAAEAGEVAGGCWQWESIKSIWRQG
                     LDSGNVSVVLQVNPKPHPELPNVPNAIDYAPNDNSKQLLKYGGHDPAAITRPYALGPG
                     TPKDRVEILRKAFVETLKDPEFLADAKKSRLDTDPLTGEEIEKIVAALFKMDASLVNQ
                     LKEILQ"
     gene            complement(20483..20953)
                     /locus_tag="VF182_18525"
     CDS             complement(20483..20953)
                     /locus_tag="VF182_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439134.1"
                     /translation="MSLDYLSIMAQAGAPKPPRVLVLPSRHAWSPVAKPLKESTVALL
                     SSAALRLENQKPFVPREDLSYRRIPADSAAGAIIIDHHSGIGRVPKQDPEIVFPRTAL
                     ASLAQRAVIGAVSPFHLSFMGGIRRSQEIENELAPAIAAELKKMDVHLALLVPY"
     gene            21287..21733
                     /locus_tag="VF182_18530"
     CDS             21287..21733
                     /locus_tag="VF182_18530"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX6439135.1"
                     /translation="MDALELLKQDHAKVKELLEKAESAEQGKERSAIFEKIKTELETH
                     ARIEENVFYPAVQKNEELKDMVLESIEEHKQVKTLLREIDSLSIDSEKFEPKLKVLKE
                     NVEHHAEEEEEGKMFPKVRKLFSANELQELGAELETAKGKRERKAS"
     gene            21776..22171
                     /locus_tag="VF182_18535"
     CDS             21776..22171
                     /locus_tag="VF182_18535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439136.1"
                     /translation="METYDVTSQTNGSQAVIDSEDRFTKSIESYTAAIPSSAYLGVAV
                     GAMALSLFCQITGRGKWGNFIAQWVPTWLIIGVYNKMVKLGGHDQFDRGIMAEPMGDY
                     TCEFCDSKFSLRSDLENHRKHCSVRTPVS"
     gene            complement(22309..22899)
                     /locus_tag="VF182_18540"
     CDS             complement(22309..22899)
                     /locus_tag="VF182_18540"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX6439137.1"
                     /translation="MTSAAQISHKSWAALALSAAVLLVFAIVVTQQIGERPAKKEAVQ
                     SDRSLDKPLEVTEQAKDVAPQAPKSDANTDAGLESTVEQLVQAWNQGDANKIAQLFLP
                     DGVLRLPTGSEIKSREEIKNTIAKHQSGMLKETTLTNDIEGVSTDGDNAVVKGSYQLD
                     GIKVLGFNASSAGSYEFQGIKRNGRWLIAKADVTRE"
     gene            23083..23538
                     /locus_tag="VF182_18545"
     CDS             23083..23538
                     /locus_tag="VF182_18545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439138.1"
                     /translation="MEIQLLKLSVDDADLNEMAPQVFVWPDAIRDIHFAVRPEGVRVT
                     GVYQQFVGIPFQMLWQISVSHGMLVARIERVTAGFFNVGFIKNYLLKFIAAATNSVIR
                     DGMLLFDIDAFIADKGWPVRLNFISVRSTLGSLVLESRARGEIDEPASQ"
     gene            23678..24370
                     /locus_tag="VF182_18550"
     CDS             23678..24370
                     /locus_tag="VF182_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX6439139.1"
                     /translation="MVDVAANYRAIVERVRQAAVSAGRNPREIKILAAAKAQDIKSIE
                     AAIMSGVPLIGENYVQEASLKRKSLPTSVEWHMIGHLQRNKAKAAVEIFDVIESLDNA
                     ELAQELDKEGRKRNIQVRALIEINIGGEETKSGVAKTKVAELLRLMGQLSHLSIQGLM
                     TVPPFRENSEETRPYFRELRQLRDELDSLQLPNVDLRELSMGMTHDYLIAVEEGATIV
                     RIGTALFGPRRT"
     gene            24378..24677
                     /locus_tag="VF182_18555"
     CDS             24378..24677
                     /locus_tag="VF182_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX6439140.1"
                     /translation="MFILANFIIATAQVLDFLLWAYQWILIARVVISWIDADPYNPIV
                     RFIYNLTEPVLEKVRERLPLIAGGFDLSPLVVWLAIWFIKRFLVQSLYDLAYSLR"
     gene            24834..26252
                     /locus_tag="VF182_18560"
     CDS             24834..26252
                     /locus_tag="VF182_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6439141.1"
                     /translation="MKIFLEGRWIDKANKIEVKNPFDNSVIDTVPKADGSDVEKALAY
                     AERGAKVMARLTSYERWKILRTTADLMAARNEELGQTISKEEGKIIAEGRGEASRAVE
                     TMMGSAEEAKRLHGETVPLDADPTGDKKLGFTLRVPCGVVAAIAPFNFPLNLVCHKVG
                     PALAAGNSVIVKPASDTPLSALKLTEMLLEAGLPPEGIQCITGSGGEIGDTLVKDRRV
                     RKITFTGSREIGDRICRMAGIKKVTMELGSNSPLIIMPDADLNRVAAAVATTGYGNAG
                     QTCISTQRVLTAKKVYGDFLDALKPKVEALSTGSQLDEKSKVGPMVKESEAVRVEDWI
                     NEAVAGGARLAVGGGRRGAIYTPAVVADVTPDMRISRDELFGPAVAVTSFENIEEAIA
                     LANDSIYGLAAGIFTENVEWAIKFAREVEAGNLHVNWGPQWRVDLMPYGGLKDSGFGK
                     EGPKYAVEEMTELKMVVFHLNS"
     gene            26322..27044
                     /gene="rph"
                     /locus_tag="VF182_18565"
     CDS             26322..27044
                     /gene="rph"
                     /locus_tag="VF182_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX6439142.1"
                     /translation="MRSDGRKPKQLRLIKIAPNYLKTADGSVLIEMGDTKVICTAKLE
                     DRVPPFLRNSGKGWITAEYGMLPGSSQARIGRESSRGKVGGRTHEIQRLIGRSLRAIT
                     DLTHLGERTVWIDCDVIQADGGTRTASITGAYVSLCQAIRSWQKRGIIAQELIKDSVA
                     AVSIGIINGKIMLDLCYEEDSKADVDMNFVMTGSGRFVEIQGTAESAPFTKKQMDKMS
                     EIAEQGIRELLKAQKQALAFTS"
     gene            27058..27678
                     /locus_tag="VF182_18570"
     CDS             27058..27678
                     /locus_tag="VF182_18570"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HEX6439143.1"
                     /translation="MIDLLVATTNSGKLAEIKSFLKYLPVHIISLSDLQDPPVVAEDG
                     QSFEENALKKARTLAEWSGYRTLADDSGLEVDALDGRPGILSARFSGIEGDDAANNSK
                     LLEEMRDVPEEKRTARFVCVLALCAPTSEGGTQCTSRGSCEGLIGFAAKGKNGFGYDP
                     LFFFLPLNKTFGEIDQQTKTTVSHRGKALKELVKVLPAWLDVVKKP"
     gene            27720..27796
                     /locus_tag="VF182_18575"
     tRNA            27720..27796
                     /locus_tag="VF182_18575"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27754..27756,aa:Pro,seq:tgg)
     gene            27865..27941
                     /locus_tag="VF182_18580"
     tRNA            27865..27941
                     /locus_tag="VF182_18580"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27899..27901,aa:Arg,seq:tct)
     gene            27957..28032
                     /locus_tag="VF182_18585"
     tRNA            27957..28032
                     /locus_tag="VF182_18585"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27990..27992,aa:His,seq:gtg)
     gene            28092..28167
                     /locus_tag="VF182_18590"
     tRNA            28092..28167
                     /locus_tag="VF182_18590"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28125..28127,aa:Lys,seq:ctt)
     gene            28420..28941
                     /locus_tag="VF182_18595"
     CDS             28420..28941
                     /locus_tag="VF182_18595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439144.1"
                     /translation="MELTATTYSMTLEVYLQSAVLRGVLVTNQDRLSNYLILREGDEV
                     LSITDATLDDLQGKAMATSSAEYLIYMQEVFLIGDLSPQVSSDRAGYEHLYVKKESNK
                     ALISVGPYWLRGDIHVIPGAPLHELLLGKTRFFPVTDAMIIGRPDVAPRTYLVNRTKI
                     GCMTAVGDGLVEL"
     gene            28965..29288
                     /locus_tag="VF182_18600"
     CDS             28965..29288
                     /locus_tag="VF182_18600"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEX6439145.1"
                     /translation="MTRRFPTEINHIKAEGVVRILWDDDHAGIYPEAYLRGYCPCAVC
                     QGHGASRKFVSVPDAKLIDIRPIGNYAIEFHWHDGHSTGIYTYDYLRSLCPCSECASS
                     VDPSQ"
     gene            29551..29634
                     /locus_tag="VF182_18605"
     tRNA            29551..29634
                     /locus_tag="VF182_18605"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29585..29587,aa:Leu,seq:tag)
     gene            29723..31042
                     /gene="tig"
                     /locus_tag="VF182_18610"
     CDS             29723..31042
                     /gene="tig"
                     /locus_tag="VF182_18610"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX6439146.1"
                     /translation="MKIDIAELSPVQRKVQIELPAEEVAGEFSRAYQDLGRRVRIKGF
                     RAGKAPRNVLQGIYGEEVREQVRSQLVEHSLGEVIKDRGLQLISRPEIEAAELQENGE
                     FSFSAVFEVKPEIDVENYLGIELDKVRVSVTGDQVGEALKRLQQSHARLEPVTDRNIA
                     QRGDFITVDFAGSADGKPFSGGKGENYVIEIGAGQALPQFDDALQGAKLGEEKQINVT
                     YPEDYPNRGLAGKTAEFSVTVREIKQKVLPTIDDDFAKDHGECSSLEELKGVIRQRLE
                     AELTQYQREELKEQLLSHLIESNPFEPPTSMVQRQTRYLLERNPTSNANSPEAETAPT
                     TEENRKALEARAVRQVQATLLVEKISQLEKIDVEDRDVQERIDKLVRAAGERGKALRD
                     FYSRAEARADLRTQIAFERTLDFLLQRAAIKEIDPPLNKVDDENKKS"
     gene            31074..31676
                     /gene="clpP"
                     /locus_tag="VF182_18615"
     CDS             31074..31676
                     /gene="clpP"
                     /locus_tag="VF182_18615"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008537172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX6439147.1"
                     /translation="MNFIPMVVEQTGRGERAYDIYSRLLKDRIVFLGSVVTDDVANLI
                     TAQFLFLESEDPERDIFFYINSPGGSVTAGLAIYDTMQYVRPQISTVCVGQAASMGAV
                     LLAAGSKTKRYALPHARIMIHQPLGGFQGQAADIDIQAREILRMREELNNILMKHTGQ
                     SLKKIEKDTDRDMFMSGKQAQEYGLVDEVIAARPEGLLKK"
     gene            31698..32951
                     /gene="clpX"
                     /locus_tag="VF182_18620"
     CDS             31698..32951
                     /gene="clpX"
                     /locus_tag="VF182_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX6439148.1"
                     /translation="MPKHGDDRSGNLVCSFCGKSQDEVRKLIAGPTVYICDECIDLCN
                     DIIAEECDHDETLTASSNVPKPAEIKRVLDQYVIGQERAKRILAVAVHNHYKRIDSQM
                     VTGDVELQKSNILLLGPTGSGKTLLAQTLARFLQVPFTIADATSLTEAGYVGEDVENI
                     ILNLLQAADYDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKIIEGTM
                     ASVPPKGGRKHPQQEFLQVDTTNILFICGGAFVGLEDIIRKRTGKKRMGFGGHLDEQD
                     GKRPTELLHETRPEDLLKFGLIPEFIGRLPVIATLDELDEFALVRILKEPKNALTKQY
                     SKLFEMESVHLKFTEEALRSIARDAMKRKSGARGLRAIMENIMLDIMYDMPSQPNIKE
                     VVISEEVVNKNEPPIVVYTKAAESA"
     gene            32965..35433
                     /gene="lon"
                     /locus_tag="VF182_18625"
     CDS             32965..35433
                     /gene="lon"
                     /locus_tag="VF182_18625"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX6439149.1"
                     /translation="MLFRSDKKDEKEKESGGSTRVPLLPLRDIIVFPHMVVPLFVGRQ
                     RSIRALEEATQKQGPVFLSSQKDAKTNEPAEDDIYKIGTLGTVVQMLKLPDGTVKVLI
                     EGKRRAQIARFVNNPEFFLVDVDEAPEVNERNTEVEALTREVHTTFENYVKLKKKIPP
                     EMVMSVSSIDDPGRLADTIVAHLGIKLEDRQNLLETFNAAERLEKVLGHMRAEIEILE
                     VERRIRSRVKKQMERSQKEYYLNEQMRAIQKELGEKDEFKNEIQEIEEKIKQKKLSSE
                     AREKIDKELKKLKMMSPMSAEATVVRNYIDWILSLPWNEFTDDKLDITEAERVLEEDH
                     YGLEKVKERILEYLAVQSLVGKIKGPILCLVGPPGVGKTSLGRSIARATGRKFVRVSL
                     GGVRDEAEIRGHRRTYIGALPGKIVQSMKKAGSSNPVFLMDEIDKMSTDFRGDPSSAL
                     LEVLDPEQNTAFNDHYLDLDYDLSKVMFITTANTLDRIPRPLQDRMEIIRIAGYTELE
                     KLSIAKRYLLEKQKEANGLTPENVTFTDNALLGVIRHYTKEAGVRSLEREIAAICRKV
                     AVEVVKKDRNTHMQVGTKSLHKYLGPVKYRYGKAEDEQKIGVTTGLAWTELGGELLAT
                     EVTVMPGKGQLIITGKLGDVMQESAQAAMSYVRSRAAELGLDKDFYQKIDVHIHVPEG
                     AIPKDGPSAGITMATSLVSALTTVAVRNDLAMTGEITLRGTVLPIGGLKEKVLAAHRA
                     GIKKVLIPAENEKDIEEIPAAVLKTVEIELVAHMDDVLKKALVIDNPENLFKRPSLSI
                     DAKEGPTPFGEKIDDNAPAEILPQ"
     gene            35537..35809
                     /locus_tag="VF182_18630"
     CDS             35537..35809
                     /locus_tag="VF182_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003659331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX6439150.1"
                     /translation="MTKADLIDSLARRAEMPRQKAEEIVNGFLDDIVAALKNGDKINV
                     SGFGTFSISERKGRTGRNPKTGEAIEIAPSRVAKFKAGKLLKEALS"
     gene            35870..35944
                     /locus_tag="VF182_18635"
     tRNA            35870..35944
                     /locus_tag="VF182_18635"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35902..35904,aa:Val,seq:tac)
     gene            35963..36039
                     /locus_tag="VF182_18640"
     tRNA            35963..36039
                     /locus_tag="VF182_18640"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35997..35999,aa:Asp,seq:gtc)
     gene            36083..36508
                     /gene="rplM"
                     /locus_tag="VF182_18645"
     CDS             36083..36508
                     /gene="rplM"
                     /locus_tag="VF182_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX6439151.1"
                     /translation="MNTEEALAQREWYIVDADGKILGRMASEIAKILRGKNKVVFTPN
                     QDTGDFVIVINAKGVRLSGHKLDDKMYYRHSNYPGGIRSRSAANLLDEKPEELIHLAV
                     KGMLPKNRLGRKLAAKLKVYAGAEHPHQAQKPKPLTIGA"
     gene            36520..36912
                     /gene="rpsI"
                     /locus_tag="VF182_18650"
     CDS             36520..36912
                     /gene="rpsI"
                     /locus_tag="VF182_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX6439152.1"
                     /translation="MAEKVITATGRRKTSVARVFLRQGKGAIAINDRTLNEYFGRETA
                     RMIVMQPFELTQTTGNYDIEVNVNGGGSSGQAGAIRHGITRALMFLNPDFRSSLKKAG
                     FVTRDPRGVERKKYGRHKARKRPQYSKR"
     gene            complement(37011..38171)
                     /gene="lptG"
                     /locus_tag="VF182_18655"
     CDS             complement(37011..38171)
                     /gene="lptG"
                     /locus_tag="VF182_18655"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEX6439153.1"
                     /translation="MARSETDKISKPLIALMQQKPRFTLLPFGSILDRYIASSFVRVF
                     IASLLCFTAIFAIVEFFERIGTLVESGISVTTALRYVLYKIPLSISRVIGFATLFSTL
                     FCLGMLSKAQEITAMQSGGLSTRRLSFPLLCLSLLICMTNFLWNEGLVPAFAHRAETI
                     YKTEIKKTQQQSLFGTKDIWIRAEGGFVNIDNFDPNKNALEGITFFVLSRDFTLQSTI
                     GVPKAQWLGQGWESESGIEWQVLPDGKLRQRIVSTPPTISETPDDLMLLARDPEEFTF
                     FDLQKQIVEMKSKGMDATAYQVDLQVKLAVPFISPLMVLVAIPLALKRRIGSGLALSF
                     GIAMLIGFAYWVLSAFCFSLGHSGALPPTIAAWLPNGISALLGIYLFTAEES"
     gene            complement(38132..39322)
                     /gene="lptF"
                     /locus_tag="VF182_18660"
     CDS             complement(38132..39322)
                     /gene="lptF"
                     /locus_tag="VF182_18660"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEX6439154.1"
                     /translation="MKRRTLTCYLLSEIFPPFFLGLLTFTFILLIARILKLVELIVTR
                     GIPLTQVGRLFVLILPTFLELTLPMAFLLAILLGLGRLSGDQELLALKASGVSPKQIL
                     IPLTFVAANVAVITLLLTSSVRPAAQVALKNELYEIARTRIGTALKEKVFNEDFPNIL
                     IYVEEIIPPGNVAQGILIVDKRDKSKDNIILGKVGLIGTEERTHTLSLRLFDGSIYER
                     DRTRSDFSQTNFNIYDFKLDLDEFIGPAKMKDAGPKEMSLSRLLQTIDEKQAAGIIAR
                     PELMEFHQRIAFSFAPLVFCLLGVSLSLLPRTSRANRSWGVALCIVWLVVYYALLSFG
                     KALGEKGTFHPFIAVWFPNIVVGVISLHLFRKALYESPLLLQTRFDAASLWLGQRLTR
                     FQNR"
     gene            39387..40154
                     /gene="rlmB"
                     /locus_tag="VF182_18665"
     CDS             39387..40154
                     /gene="rlmB"
                     /locus_tag="VF182_18665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX6439155.1"
                     /translation="MENEFIIGIHPILEKLKASPEEISVILLARGPLRPTLKSLESEA
                     QKLQIKIQFMDAKELDYLALGQSHQGALARVRPYSYLPLSELERTLASEASPQRIVIL
                     DGITDPRNFGAILRTAEAVAVGHIIIPKDRSVRITPIVLRTSAGAVNHLRIYRVTNLR
                     RAIASLKKSGFWIAGLDANARTSYETIKYPDKLGIVLGSEGRGLRPLISYECDYLVRI
                     PMKGRITSLNVSVAAAILLYEVSRQHGDIDKGKPNGY"
     gene            40373..40447
                     /locus_tag="VF182_18670"
     tRNA            40373..40447
                     /locus_tag="VF182_18670"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40405..40407,aa:Thr,seq:tgt)
     gene            40524..40608
                     /locus_tag="VF182_18675"
     tRNA            40524..40608
                     /locus_tag="VF182_18675"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40558..40560,aa:Tyr,seq:gta)
     gene            40633..40708
                     /locus_tag="VF182_18680"
     tRNA            40633..40708
                     /locus_tag="VF182_18680"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40666..40668,aa:Gly,seq:tcc)
     gene            40786..40858
                     /locus_tag="VF182_18685"
     tRNA            40786..40858
                     /locus_tag="VF182_18685"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40819..40821,aa:Thr,seq:ggt)
     gene            40921..42111
                     /gene="tuf"
                     /locus_tag="VF182_18690"
     CDS             40921..42111
                     /gene="tuf"
                     /locus_tag="VF182_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX6439156.1"
                     /translation="MSKQKFERKKPHLNIGTIGHVDHGKTTLTAAITKVLSKQGKAQF
                     LAYDQIDKAPEERERGVTINIAHVEYETDKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPGIVVFLNKADMVDDKELLELVELEVRELLSK
                     YKFPGDEVPIVIGSALKALEGDASDMGEGSIIKLMEAVDSFIKEPTRDVDKPFIMPVE
                     DVFTISGRGTVVTGRVERGVIKVGEEIEIVGFKPTQKTVATGIEMFRKLLDEGRAGDN
                     IGVLLRGTKREEVERGQVLAKPASITPHTKFNAEAYVLTKEEGGRHTPFFNGYRPQFY
                     FRTTDVTGVVKLPEGTEMVMPGDNINVEIELITPVAMDEGLKFAIREGGRTVGAGVVT
                     KIIQ"
     gene            42135..42287
                     /gene="rpmG"
                     /locus_tag="VF182_18695"
     CDS             42135..42287
                     /gene="rpmG"
                     /locus_tag="VF182_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX6439157.1"
                     /translation="MRDIVGFACDQCKRKNYTSTKNKKKTTDKLSFKKFCASCRSHTI
                     HREIKV"
     gene            42300..42377
                     /locus_tag="VF182_18700"
     tRNA            42300..42377
                     /locus_tag="VF182_18700"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42335..42337,aa:Trp,seq:cca)
     gene            42397..42612
                     /gene="secE"
                     /locus_tag="VF182_18705"
     CDS             42397..42612
                     /gene="secE"
                     /locus_tag="VF182_18705"
                     /inference="COORDINATES: protein motif:HMM:NF012793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX6439158.1"
                     /translation="MADWIQKLQDTLRHTIEFVKESWQELQKVHWPSRKETYAATALV
                     IIVVVLVSVYLAIIDLGLTTMIQAIID"
     gene            42629..43159
                     /gene="nusG"
                     /locus_tag="VF182_18710"
     CDS             42629..43159
                     /gene="nusG"
                     /locus_tag="VF182_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX6439159.1"
                     /translation="MAKQWYIVHTFSGFEQKAKAALEERVRTLGKQEYFGEILVPVEK
                     VVELVKGKKKTSSRKFFPGYILVQMELNDDTWHVVKETPKITGFVGGSTNPPSISEEE
                     VRAITLQMEEGAIKPKPRVLFSVGENVKVVDGPFADFNGVVEEVRPDKGKLRVLISIF
                     GRATPVELEFVQVERN"
     gene            43177..43602
                     /gene="rplK"
                     /locus_tag="VF182_18715"
     CDS             43177..43602
                     /gene="rplK"
                     /locus_tag="VF182_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015416562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX6439160.1"
                     /translation="MAKKVIAEIKLQIPAGAANPSPPVGPALGQRGVNIMEFCKAFNA
                     ATQSQQGMITPVIITVYADRSFTFVTKTPPASVLLKKAAGVEKGSKEPGKTKVGKVTR
                     AQVRNIAELKLKDLTAKDLAAAEKSVEGTARSLGLDVEG"
     gene            43665..44369
                     /gene="rplA"
                     /locus_tag="VF182_18720"
     CDS             43665..44369
                     /gene="rplA"
                     /locus_tag="VF182_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX6439161.1"
                     /translation="MSGFGKSYRAALAKVDRSQRYQLDEGLQLVRQTARAKFDETVEL
                     AIRLGVDPRQADQNVRGTVTLPHGMGKAVRVLAFAKGEKEKEAQEAGADFVGSDELIK
                     KISEGWFEFDKAVATPDMMAAVGRIGKVLGPRGLMPNPKTGTVSADIARAVKEIKAGK
                     LEFRVDKAGIVHVPVGKASFEPEKLIDNARAVLSAILRAKPASAKGNYVRGVTLSTTM
                     GPGVKIDLSQIRAMAA"
     gene            44415..44957
                     /gene="rplJ"
                     /locus_tag="VF182_18725"
     CDS             44415..44957
                     /gene="rplJ"
                     /locus_tag="VF182_18725"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX6439162.1"
                     /translation="MAELEAARSSHGEKAETVAAVHEKFRKAKMAIATEYRGLSVSKM
                     TQLRREIRDAAGEYQVIKNTLVRRALKDTAYGDLERMLEGPNGWVFAYDDPVLLSKTL
                     VKFVDANEAMTIKAAMFEGELMDPARVKVLSQMPSKPELQATLLALLQTPATYLLRLI
                     QEPGSRVVRLLDAAAKEKAG"
     gene            45003..45380
                     /gene="rplL"
                     /locus_tag="VF182_18730"
     CDS             45003..45380
                     /gene="rplL"
                     /locus_tag="VF182_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX6439163.1"
                     /translation="MAEVSREQVKDYIKNMTLLDAATLVKELEEELGVSAAAPVAVAA
                     AASGPAAAAEEKTEFDVVLSEAGEKKIQVIKVVRELTGLGLKEAKDLVDGAPKNVKEG
                     IAKAEAETIKKKLEEQGAKVEIK"
     gene            45447..>48753
                     /gene="rpoB"
                     /locus_tag="VF182_18735"
     CDS             45447..>48753
                     /gene="rpoB"
                     /locus_tag="VF182_18735"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX6439164.1"
                     /translation="MPFQVANNLRFRRSFGRIKRIIDLPYLIEIQKNSYELFLQKDAS
                     AAQRENIGLQEVFKSVFPIKDFNETASLEFVSYSLGEPKYDVDECHQRGMTFAAPLKV
                     TVQLVLWDVDAQTGARSIKNVKEQEVYFGEIPLMTKNGTFMVNGTERVIVSQLHRSPG
                     VFFEHDKGKTHASGKLLYSARIIPYRGSWVDLEFDPRDVVYVRIDRRRKFHATVLLRA
                     LGMTTEDLLNYYYRSDTIVFDGRKPALIFNPAHLMGAKVTSDVRDPKSNDLIVKEGKK
                     FTRPVLRQMEQAKVKQVPIALEDILGRIASHDIVNPQSKEVIVESNHPITQEKLDLIR
                     EKGIHQIEVLFIDESTVGPYLRNTLLQDRINSPQDAILEIYRRLRPGDPPTVDSATNF
                     FNNLFFNPDRYDLSKVGRLKLNHRLKLNVPLEQGTLRKEDILEVVRYLMELKNGNGTV
                     DDIDHLGNRRVRAVGELVENHFRVGLVRMERAIKEKMSLQDIETLMPQELINYKPVSA
                     ALKEFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHYGRV
                     CPIETPEGPNIGLIASLTTYARVNEFGFIETPYREVDNGKVTDRIRYLSALEEEDHVI
                     AQANAPIDARGVFTLDLVSARKGGEFVMARPEEVNFMDVSPNQLVSVAASLIPFLEND
                     DANRALMGSNMQRQAVPLLRTEAPLVGTGMEKVVARDSGVTVVAKREGIIESVDSTRI
                     VVKADKASGPRDTGVDIYNLVKYQRSNQNTCVNQRPIVRIGDQVKAGDVIADGPSTEM
                     GELALGRNVLVALMPWGGYNFEDSILISERVVKEDIYTSIHIEEFECVSRDTKLGPEE
                     ITRDIPNVGDEALTDLDDSGIIRIGAEVKPSDILVGKITPKGETQLSPEEKLLRAIFG
                     EKAGEVRDTSLRVPPGVEGTVINVRIFSRKGMGKDERSKAIEDEEIIRLRKDQHDEVR
                     ILQDGALRRLKKLLVGRKLANRLSDDSRKLLLVKGKEITEEDLDQLPMSYWSEIRVDN
                     ESVEAELDRIVESTAGQVKLIKEVFEDKIEKLRGGDELPPGVIKMVKVFVAIKRKLQV
                     GDKMAGRHGNKGVI"
BASE COUNT        11900 a        12335 c        13382 g        11136 t
ORIGIN      
        1 ctcttcacta agactgcgcc gagttgcttg gccagccgga accgagccct gagatcttgg
       61 tggcgattcg ctcccggtca aagtgtaaag gagatcgtta gcccaggggt aagacataac
      121 tgcggcggac atcgtgagca ctacaagcac gctggaagac cagaagccga tcacattgtg
      181 ccagttccag tcgcgtgctt tgccgcctag gcgaggattg aaaaggaggc tggatttcaa
      241 tccccgccag cgccaactgc gcggccacca gagataaacg ccggtgatgg ccagccagaa
      301 gaaagcgaga ttgcacgcgc cggtgatcgc gcgtcccgtc gctcgacctt caccgtcttg
      361 accgagccaa cggtgccagt ccgtgacatc gtgaaaccaa cgctggagtt tagattcttg
      421 tcctaacact gcgccggtgt atagatcgac atagacagcg ccttcgcggc cgaagccgac
      481 ggcaaccgga gccgtgggct gattgcgcag aaccacagcc gtcggctgcg cattaagatt
      541 ttgcgcccgt gtcttcgcga tcacctcgtc caagcttagc cgcgctgtgc caatccgagg
      601 tgggacctcg cgcacgttgc gctcggcata ttccacaatc tgatgctcgt acatgaggat
      661 gacgcccgtg gccgacatca gaaaaatcac cgtcccggca accacgcccg cgatcaggtg
      721 agaccaaaag ataattttgt gaatcacgtt cattcgtaaa ctactcggac cgtccgatat
      781 tttatgcttc acacttccgc gaagcgccgc atcaggttgt ggtagcaatt ggtcaactcc
      841 accacggcac cgtgattggg cagggcttga ttgactttgt tgatggccat gtcgagatcg
      901 aagagcaaag tgcggttgcc gtcgtcgcgg accatgcttt gaacccagaa gaacgacgcc
      961 agacgcgcac cgcgcgtcac cgggttaacg tggtgcagac tgctcgccgg atagagaacc
     1021 atatcgccgg caggaagctt caccgcgtgt gcgccgtaaa cgtcgtcgat caccagctcg
     1081 ccgccgtcat actcttctgg cgcgctcaaa aacagcgtcg ccgaaatgtc cgtgcgcacg
     1141 cgcagcggcg tgccgggaat ctgccggatc gagttgtcaa ggtgagcgcc gaacgactcg
     1201 ccgccttcat agcgattgaa cagcggtgga aagacgcgca gcggcaaggt cgcggtgatg
     1261 aacagcggat tgcgctccaa tgcggcaaca atcatgtctc ccagctcgcg cgcttcggcg
     1321 ctgttctccg ggagctggcg attgtctttg acctgcgccg actgatgccc ggccgtcacc
     1381 cggccgtcga cccagctcgc gcgctccatc acttggcggc agcgcgtgag ctgttggttc
     1441 ttgagtacgt tgggtacttg gacgatcatg ttggcaacgc tcctaaaact tgaaactcgc
     1501 gctggcgata aacgtgcgtc ccgcgcccgg aacagccttt gaaccggcta cactgtcgaa
     1561 atacaattgg ttggttagat tgatagcatt gattctcagc tgaatattgc gtgtgatctg
     1621 ataggcaatg gtagaatccc accggacaaa gccaggaatc cggttggtat tcgcgttatt
     1681 agaaaaacgg cttccaacat aggtcggtcc accgccgatc tgccactttt caaaaaaatc
     1741 ataggtggtc catagcgtgg cgctatgttg aggcacgttg gcaatctcgt tgccgacatt
     1801 ggcggcggtg ttatcttcga gaatctccgt atccaaatac gtgtaaccgc cgaaaacgtt
     1861 gagccccgga accagccgcc ctgttagccc aacctcgaaa ccttgtgagc gttgttctcc
     1921 ggtcacgatg ttgggacccg tcggatcgtc gggattgatc gtgcgcgcgt tggtcttttc
     1981 gattcggaat gcagcagtct ggaaattaag cgcgccttca aaaaattcca gcttcgcgcc
     2041 agcttccaaa attcggtttt tttccggcgg cgtgccgttc gtagtagggg taagagcgat
     2101 accttcggca gacgggttga aggatgtacc gtaggaaaaa tagtagcttt gcgaggccgt
     2161 cggatgaaac actaggccgc cgcgatagct ccacatttgg tcgatctggt tacgcgtgtc
     2221 atcggcaaaa tcgtccttga tgtgagcgct aaagtaatcc cagcgcgctc ccccgaggat
     2281 atcgaaatag ctattcagct tgatctgatc tgcggcgtag atgccgaacc ctgtggcacg
     2341 cgtgtcggag tccgcagcta gggccttcgg cgcgggcagt tgatggctat cgggattttc
     2401 gatcgtcgta tccggtccgg cgctgttcca tcggctcagg tcgaatgttt cgcgcgagac
     2461 ctctacaccc gtggtggctg tgtggccgaa tccaaaggta tcgaacttcg cgatgaggtc
     2521 agtctgatta ccaaggatgc tttcctgcgt atcgcgctgt gggcggctgc gattgagcgt
     2581 gttgggaaga accgcggtcg cgttcgtcac cgcggaatcg cgatcgacat ggctgtagcg
     2641 taaagtattt ctcagattga agatgtcatt gaaacgatga tcgaatcgca gagtgccgac
     2701 attgacccag gtctcttcgt ggtcttcctt gtttaggcca tagaacgtgt cacgatcgat
     2761 tcgcagaggt ttaccgtcgg cataaggaaa accgtaatcc ggaatattgt cttcatgctg
     2821 gagaaggtag ctcgcagtga gttgggtcgg cgtgccgagg ccgaaggaaa tcgaaggagc
     2881 aaatcccaag cggtttcctt caattttatc gcgatccacg acatcggctc tttgggccat
     2941 cagattgagg cgcaatgcgg cgttcgggag agcgtctttc ggaagcggtt gattcacgtc
     3001 gatggtgccc cgcaaaaaca gagcgctgcc tccgctgaac attccttccc agcttgggtc
     3061 aagccggggc accttgctaa cttgattgat aatgccgccg gtggaaccac ggccaaaaaa
     3121 cgttgacgac ggacctttca aaacttctac ggactcaaaa ttgaatgaat cgcgaaagta
     3181 agaaccggcg tcgcggacgc cgtctatgaa catatcgttg cgggcattga atccgcgcaa
     3241 cgtaaagctg tcaccctgcg acccgccgcc ttcaccggcc gcaaccgcga ttcctggcac
     3301 gttgcgtagg gcatcgcgca acgtggtgcc agcctgctct tcgatcagct tctccggcac
     3361 gatggtgatg gattgcggcg tatccttgag cggcacagga aactttgaca gcgacgattg
     3421 cggcaccacg taggggctat tctgatcctg cacgacgacc ggaggcaact ggaatgtttc
     3481 ttctttcttt ggctccgccg cgacggcgtt gctccccagc gcgaagctca aagccagcgc
     3541 cttaccaacc ccctcacccg cagcaagaca aatttttcga agtgcggtct tgcgctggtc
     3601 ttgttcgagg gcgcgccttt gttgtcgact catgcatcct ccctgaagtt aagattgctc
     3661 tataaggctg gctttccgtt ccggaggatt ggatggctgg cgtgtgcaaa ctttatttcg
     3721 ttaaaataag ctttccgttt ctggtgattc gcaggttata ttcctccctc tgatgcacga
     3781 tcaccacttc agtcttcccc tgaaacaagc tgtcgctttg gatgcgcggc tttttgtctg
     3841 tgagcactgc aactcgcgtc acaatcgctt gttcaggcgc aacctttagg tctgattctc
     3901 ccatgggatt tctaattcaa ctcgaattta accggcaaca tcacccacac tgaaacaggc
     3961 tccttgccgc gccgtgccgg ctcgaaacgc cattttttta ccgcgtccat cgcggcgaga
     4021 tcgaggtccc gaaaacctgc cgactcatcg accataactt cgccaacccg gccgctctcc
     4081 agcacatgga cacgcaagcg ggtgacacct tgagcaccgg cgcgacgtgc cgacgccgga
     4141 tagcgcggtt taacctggta gccgccgatt ggccgagcag tacctgagag agcttctccc
     4201 gaattcgctc ccccgcctcc cgtgccatcg ccttcggttc gttttcccag cccagagatg
     4261 ccctctccac cgccgccacc ttcgataccg gttccgccca ccacgccaac ttcgccgttg
     4321 tcaaacagat tcccggcacc ggccgcgctg cctttggctt ggcccggact atcagcagcg
     4381 ttccaaccgc cttcactggg ccccggattc gcgggtaatg cagctggtgc cgggggtggc
     4441 gatttaacca tgggttcggg ctgtttgatg tcttccgggg gttttcctgg tacgggtacc
     4501 ggcttggttt cgataatctt cggcttggag gtgagttttg gcgcggtaat ctcttgcggt
     4561 tttggcttga tcctgggcat cgatggaggc tctggcgccg gcgcgagctg gatcttgggc
     4621 cgctcggctg gcgaaatctc gatcaattcg ataggcacct tgatcggctt ggccggtctt
     4681 gtatggacca tcagagccgc caacgagatc agcgccaaat ggatgagcga ggatgccgtc
     4741 aatgatgaaa gcaaccagcg atcaacggtc attggactcc tgctttggtt tcaccgcgat
     4801 cgccattctg gcgatgttag cggtccgagc gatgtccatg acttccacca cacggccatg
     4861 ctccacgcca tcatcggcgt tgatgacgac taacaagtcg gcattctggg cgagttgctg
     4921 gcgcagcaga tcacctagcg tcgtcttctc gatcggctgt ttatcaagaa aaagccggcc
     4981 ttgactatcg atcgtgatgg cggcgctttc ggcgggtgct tgctggcccg aggccgcttt
     5041 cggaaggtta accggcatgc cgcggtactg ggccatcgat aacgtcgaga tcatgaaaaa
     5101 gaccaacaag aaaaaaatcg tgtcgatcat cgggatgatc tcgatgcgcg ctttcttgat
     5161 gctgcgtcgc tgaatacgca tcagtgcttt ccggcgtcct cgtggagcag cagctcgaga
     5221 gacgagctat agtgctccat ggcgtccatt tctttttcca cccgcgagct gaagtagttg
     5281 tagggaatca aggtcaggat cgcgatcagc aggcccgtag cggtagcgat caatgcttcg
     5341 gcaacgccgc cggtaaccgc gtgtggttga cccatgccgg cttcagacat gatgtcgaaa
     5401 gagccgatca tcccgaccac tgtcccgagc aggccgagaa gcggagccaa ggtgatgatc
     5461 gtgtcgaggg cgccgagcct tttcttgagc accgggatct gcgcttgcgc ggcggcttcc
     5521 atcgctttcg aaggccccgg aatgcggtgt gtcaatcccg ccgcgagcac ccgcgcaatc
     5581 gggtgctgcg attctttgcc gagctttatg gccctgtcga attcgcccgc ttccgcgaga
     5641 ccgaacatgt gctgaacagt ctgcggggat ttgatcttgg cccagaaaaa gaaccgctcg
     5701 atgctgagcg cgagcgatat tagcgagcac gcgagcagcg gaaccataac gatgccgccc
     5761 ttgatgatga tagcaactac gtcggacatc ctaccggacc aaacctttct aaagatccaa
     5821 gtgatcgctc gactgcgtat tcgcagcatt tttcggcgac gctctgcgtc gcggcggagt
     5881 tcgcgcagat cctcgagtga aatatgcagg acaatgttgc cgtggtggaa gcgaaccgtg
     5941 tcgcggctgc agctgcacaa cgaaaccgac cattttctgc atgcgattta atttcctgca
     6001 tcgtgtcctc caattaaaat aagcccggaa agccgccgaa tatctgacgc ataccggggc
     6061 tctggactcg aagatcgcgg gccgctgctt ggttcgttta tccaaattaa ttttctttgt
     6121 caagacgaat aataatgttg tgtggtgacc aactattttc catcgctggc aacaaaacgt
     6181 aatgaaacta acagcctata ccaaatctaa actctctcct ggcaagcgaa atacgatcgg
     6241 ttccttgcac ctgccagcaa actgctgcga tggccgcgca gcgctttacc ggccctaacg
     6301 gctgagtcgc ggcctgagat tcgtgggatc gtccggccat ctacgcaacg gccttttttc
     6361 gttcgtggcc catcgagaat aacgtcacga taaatgcgca gagcaataaa aattgcaccg
     6421 tcagtgtctc tatcgtcgga taaatcccta aaagcggcac agctggtggg aactccagtg
     6481 gtgttgccgg cacccagccg gccgcctgca gttggttgat tccggtaccg gcgaagatga
     6541 atgccatgat gtaaagcaat gttccagtga tgccgaaaaa atatttcagc ggaatgcaca
     6601 cgccgagttt gaaaatcgcg aacgtcgccg ccagcagcac aatgacacca ctgacgaagc
     6661 cgccgacaat cgattgtcgg ctagcttcgt tttggagcca tagcgcctga tagaacagca
     6721 ctacctcgaa cgcttcccgg tagacgacga agaaggagat accgacgagc ccaagaatgc
     6781 gcttgctcga caaagcattc tggacgcggt cctggataaa cgcctgccag cgcttggctt
     6841 cgctccgggt gtgcagccaa tagccaacat aaaacaacgc taccgcggca aaaatcgaga
     6901 tgaatccttc catgctctcg cgatgctgac cgctgaatgt gaggatggtc tgcgccgcca
     6961 accaggtgag cacaccgccg aagagcgcga ggatccagcc taagtgaatg tagcgaatcg
     7021 catcggacgc gcccataact ttcagcattg cgacgatggc agcgaggatc agagccgcct
     7081 cgagaccttc gcggagaatg atcaatgccg aattcaaaaa tgcataataa ccaggaaacc
     7141 caccgccgcc ggccgccgtc agtttctgcg ccgccagatc gagattggct tcgattgtct
     7201 gaagtttctt ctggagatct gcgagcggca caccctgcct gatggcgttg cgaaactcca
     7261 tgaactgcga ctcgagcgtt tttcctaatg ctgcatcttt agcgaacaag gcgggctcag
     7321 ccatctcgaa gccgtcgagg taggcttcga tcgctttctg atacgacttc tccttatcgc
     7381 cgcctgcgta gagattcgta gcctcgccga ccagtgtgcg ggcgatcaac aacggatccg
     7441 aggttttctt ctccaaaagc ccgcggcgga aataagcgag gactttctct ccaggcgcat
     7501 ccggaaagtg cttcttgatc ctgcccgaca aatcttcatc ggaagaggtc gccaaagtct
     7561 cggtgctggt gagatcgccg gggatagctt tgcctttgag ccaagcctcg cccgcctcag
     7621 catcctcggc cgaaaatcgt aacgccatga cataaaatgc gagctgccag cgctgctcat
     7681 ccggtagcgg cgcgaacgat gccatcgcgg tgttgtcgac tccgaaagtt gtcgtgttgt
     7741 aaaccttgaa tggtgagagt ccggtcatga agtctggatt cgtgaaattg gcgggtgacg
     7801 gagttcgagg attcatcgac tcgcggccgg gcccgtcgcc tttgccagtc tcgccgtggc
     7861 actgggcaca gttctccagg tagagcgtct ttgcgatgac gtagttgggc aatagtttgg
     7921 ggtaagtggc aatgttgtag gttttgatca gccggtcttt gatctgttgc gcgagctccg
     7981 ggacggcgtc ttcgccggac ttgtttttga tatgggcact aatcacggtg agatctttct
     8041 cgatcaccgc cttgtcacct tcggcggact tgagctggcg gaacagttcc aggctgcgcg
     8101 ccgagaactc gctcatctct tgatactcgt catcgttcac gacttttcca ttctgcacgg
     8161 cgttgcggta atcgccgccg atatagtcga tcagcgccac aatgcggtta gcttcgtccg
     8221 cggcccacag tgtcgaagcc gaaaacacga ccagcaggag taccagagtc atctgacgga
     8281 ggcgcatgaa accactattt tgatgcggtg cactcatgtc ggctcctccc gtctcgaacg
     8341 agcgatctgg cgtgaaagga aaaccagtgg taacagcgca atcgtcacca tgacgagcga
     8401 aggaaccgcg gcttcggcaa tgcgctcgtc cttggcgaga ttgtaggcct ccacagcaag
     8461 tgtatcgaaa ttgaacggcc gcatggcgaa agtcgccggc agctctttca tgacatcgac
     8521 aaacacgagc aggaccgcag tcgccagact gccagaaatg atcggcacgt ggacgcgcca
     8581 caaagtatgg acgggagaat agccaaggct gcgggctgcc tcgtccatcg agagagtaac
     8641 gcgcgtcagt ccggcttccg cagtatggaa agcgacggca agaaaccgca ctacgtaggc
     8701 atagatcaac gcaaccatcg aacccgtaag aagcaggccg gccttgatcc ctaactgttc
     8761 ctgcacccag ccagcaaggg tgttgtccag cttccccaac ggcagcagaa tacccactgc
     8821 gatcaccgcg ccgggaagcg catagccgag cacggcaaag cggtttgcga tgtgaaccaa
     8881 cgggtgctta accgtccggc ccgcatatgc cagaagcaag gccaccgcga ctgcaacgaa
     8941 cgctgcaact cccgctaaag tgaatgtatt gagaacaagc ccgtagaggc gtgcgccgaa
     9001 ctgcgcttcg gggtctcgaa gcgcaagcct gatcagcaac gccgcaggca aaaaaaatcc
     9061 gaacgtcacg ggcagagcgc atgcaatcga ggcggcagcg gcggccgggc cgtacagtag
     9121 ctgcggctgc atggggatgc gcttcgcagc ataccgagca ccccctcgat ggcgttgctc
     9181 ccacgaaagc attagcagga cgaaaaccaa caagcatgtg gccagctgcc ccgccgctac
     9241 cggatcgccc aatgaaagcc aggcgcgata gattcctgcc gtgaaggtgt taacggcgaa
     9301 gtaagatacc gtgccaaagt cggccagggc ttccatcaat gccaacgccg tccccgcaac
     9361 gatcccggtg cgggcaagcg gcagcgcaac acccaggaat cgaccccagt cgccgtaacc
     9421 agctagccga gccacttcca ttgttgtgcg tgattgttcc aaaaacgccg tgcgtgcgag
     9481 caagtaaacg tacggataaa gcgcaaaggt caacatcgcc gcggcaccag gcagcgaacg
     9541 gatctccgga aaccaatatt cacgaacttt caacccggtg agttcgcgaa acacggattg
     9601 caccggaccg gccgcttgga gccaatcggt atatacgtac gccatcacgt aggccggcgt
     9661 cgcaagcggc agcaccagcg cccactcaag cgctcgttgc ccagggaacc ggtacgcgct
     9721 cacgagccac gctgtggtca cgccgaacaa cacaacaccg atggcgacga gcaaaaccaa
     9781 ctgaagcgta ttccagatat attcgggtaa gacagtcgct agcagatgag accacgtggc
     9841 cttgctcggc aaaaataggt tggagacaac cgccgcgatt ggcgcgagaa ccagacatgc
     9901 aacggtgatc gaagaccagc cgagccaacc cagcttgacg cccctaatac cattaacgaa
     9961 tcggtagcaa acggctgcag cggtcgtcat gtgcgaatca ctgccgacga cgccggctta
    10021 cttccagccc accttgtcgg cgatccgctg ggccgcagcc tgtgatttgc cgagattggc
    10081 gagcggcaaa gaatccgtct tgaactggcc caaagattcc aacgcggcat tgtccagctt
    10141 cacgcccttg acgattggcc attcattgtt gccgttggcg aacaaagttt gggcggcatc
    10201 gctcgccagg tattctaaaa acttaatgcc tgcattccgg ttcggcgcgg tttgcagaag
    10261 accgcccccg gagatgttca gatgcgttcc gcgatcggcc tggttcggcc agatcagacc
    10321 taccttctcg accagcgctc gctcttcagg tttgtcggag cgcatcaggc ggacataata
    10381 atagtggttt gagatcgcga tcgagcattc acccacctcg accgccttga gttgatcggt
    10441 gtctccgccc cggggtggcc gtgcaagatt agccgtcacc ccacgcgccc attccgtcgc
    10501 cttctcctca ccgagatgac tgatcaggct cgaaatcaga gacaagttgt atggatggct
    10561 gcttggccgt acacagatct gtcccttcca tttcgcattt gccaggtctt cgtagcgagc
    10621 taactcggct ggtttaattt tgcttttgtc ataagcaatc acgcgagcgc gtgcggaaaa
    10681 tccaaaccac ttgttatcgg cgccactcgc gttggcagga atgcgcttgt cgatgatcga
    10741 ggacttgacc ggggcgaaga tcccggcttg ctctgcgcgc cacaagcgcg cagcgtcgac
    10801 cgtcaaaaag acatcggcgg gactattggc gccttctgct ttgatgcgct cgaaaagggc
    10861 gtcctcgccg ccttcgatac gattgatctt gatacccgtt gctttcgtat agcctgtata
    10921 gaaagcttcg tcggtggcgt agtggcgcgc ggtatatagg ttcagcactt tctcttgggc
    10981 tcgcgcctcg ccaatcgatg aagtcagcac gcatgtggca atcgaaagaa tcgaagcgaa
    11041 aatttttctc ctgttacctg cgggcgcatg catgtttcca ttcccctttc gaacacctga
    11101 aaatgtggat ttgattgctg gatcgcattg tttgccatat caaactattt attttcgagt
    11161 atgcgaatga tcggagtttg tcaaacacac ggaggttgtg caagacacct gcttggtgcc
    11221 actatctttg tcagacgggt aatgttgaga tagttccgga agtgattatc caggcgctgg
    11281 gctagacccg ggctcgggaa aaacgacgac gttgagaaga tgcagttcga ctgctacctg
    11341 atgcccaagc ggccaatgca ccggctggcg ctgttccaaa tgaatcatcg ctcctgaagg
    11401 aagacgcaat cgatatagta tctcgccgcc aaggaattca cgttcttcga cggtggcaag
    11461 cacaccggca gttttggaat taaccgcctc gtgacggacc atgagcttgg cgggaccgtc
    11521 agccagttct acagaacagg gaaaaacacc aagctcggta tggatcctcg accctagcac
    11581 ctggccggga atgaaatccg cctggccgac gaaattagcc acgaaaggtg tagtcggcga
    11641 gtggtaaatg tcttcgggag tgcctacctg ctgaagttca ccgttgcgaa tgacggcaac
    11701 gtgatccgcc attcccagcg cttcttcctg atcatgcgtg accagaattg ccgccgttcc
    11761 caactgccga agaattctgg ctacctcccg gcgcatctgg ggccgtaggt caggatcgag
    11821 accattaaaa ggttcatcca gaaggaccac gcccggccgc ggcgcgagcg cgcgcgcgag
    11881 tgccacccgc tgctgttgac cgccggatag ctggtgggta tagcggtctg cgcagtttgt
    11941 caattccgtt agtcgaagga gttcttcgac ccggcggcga cgctctgttt tactcgagtt
    12001 gcgcaaaccg aaacgaacgt tttgtgcgac ggtcagatga gggaaaagcg cataatcttg
    12061 aaagaccatg ccgacgcctc gatcctctgg ggaaacccaa atcgatttct ccacgtcgac
    12121 gacggtgcgt ccattgatga cgatcaagcc ttcgtcaggt ctctcgaatc cagcgatcaa
    12181 cctgagcagt gtggtctttc ctgaaccgga cgggcccagc agcgccaaaa ttttgcctga
    12241 cgcaacctgc aaatcacagc tttttaccgc aaccgtcttt ttgtaacgct taccgatttc
    12301 tttaacttcg atgatgacca taacttttcc cgtcagtctt aataactgaa actgtaaagc
    12361 gggtctagct tattgggcga tatgggccga gctgtgcgga gatcctattg atcttcggtc
    12421 atctgatcat cgccttgaac ttcagtaccg tctgaaatag gatatggcct gcgtgcaaaa
    12481 tccacacagc agttctggct gcaacgaaac atgcgggcgc acgttccggt gttcttatct
    12541 ttaaaaaatt ttaacagtga tcatggaagc tcatcacgta acgcatgcgc ttgaagacta
    12601 tttgaaagcg atttaccagc tttccgagga ggatcgaccg gtgattgccg ccaggctcgc
    12661 cactgaaacc ggcgtatctc cgtcaacgat ttttgccacc ctgcgtcgct tggccaagga
    12721 aggttatata aagatcgatc gtcgcaagga aattcatctg acggaagccg gcaaacaggt
    12781 cgcggaaaac atcgtacgcc gacattttct gacggagcga tttctcactg acttgctggg
    12841 tctggattgg gttaaagcgc atcaggaagc gcaccgtcta gagcatgcca tctcacagga
    12901 agtagaggaa cggctcgcca agctgctcag ccatccgacg acttgccccc acggcaaccc
    12961 cattccaggg gcgagctcga cacccggacc gaagacaatg ccgctcagcg aagcggctga
    13021 cggacaggac gttgagctcg attatattac tgagggtggc gagcgcgatg tgcgcttgct
    13081 cggctatctc caagcgcatg ggttacgacc cggcgcgaaa gttcacatcg tcgatgtcgc
    13141 gccctcgctc ggcatgatga ctttgacggt caacggcgac gagttttcgc ttgggatcga
    13201 ggcggcaaag aaaatccgcg tgcactgata tcgatcgact ccagtgctgc gttcaattgt
    13261 cgaacggaat agaaacggat cgaacccata gtgatcgtcg acatcgatga tcactatgga
    13321 cttgtaatgc tccaggggtt gatcatgtgc gagcggatgg ttgactttct tcttcagcga
    13381 gaattgctgc tagaaaatcg atttgctcgc taagcggcgc gtcccggtcg atcaggatca
    13441 attcctggcc attgatacga caacgcccgc tgcgcggctt gtatcctact tcacgcaata
    13501 gcttttcagt gcgcacctcg atgttcatct gtttggcggc gcccaccagt tcctgcaaac
    13561 gtaattgctg cttatgtttt atctgtgcca tcgctgaatt caaacgctgc agcattatct
    13621 ccgaaagtcg ctgatttgta aaacgattga cccattttcg gcttgcaaaa accgagcagg
    13681 ttgttatcgt agcccgcgtt gatcggagaa tgccgctcgt aacccggagc cgttgcgctg
    13741 atgcatgcac agcatgtcgc catacggttc aggagggaaa cgtgatcaag aatattttac
    13801 ttccgctcga cggctcggaa cactcgcagg cagccttcga ctatggtttg tggatgacgg
    13861 agaaattcga cggaacacta ttcggtcaac acgttatcga tactatctcg atcgaaggca
    13921 cattttttca cgacatttcc ggatccatgg gattcgaacc gtacctcgat ttctccacaa
    13981 aaatgcgcga agccttggag gagcgcggca agttaatact cgaagaattt gcccaacgtt
    14041 gccggaagag cggcatcaaa catcaaactg ttttggatat gggcttaatc gccaacgaga
    14101 tctgcgaacg ctcaaaaatc gccgatctgg tcgtcattgg tcaccgggga attaatgagg
    14161 attttaccac cgggctcttg gggacgacgg cggagagcat cacgcgcaaa tgcccgcgcc
    14221 cggttttcgt ttcgacaaaa aaattcaaac ccatcgagag accgctgtta gcgtatgacg
    14281 gcagccagcg cgcctgcgcg gcaatggagt ccgcggccga gttctgcacg gtattgagcc
    14341 tgcccctgag cgtgttgacg atcgccaagg aagatcagct gggcgaagga attctgcatc
    14401 aagcgaaatc atatttaaat tcatattcga ttgatgttca ttacgaaaag gggcgtggat
    14461 atccggaaca gaagatcatc gatcacttgg tcaatttcaa tcatgatctt ctatttatcg
    14521 gtgcttacgg ccatcggcgg atcatcgaaa tggtgctcgg tagcacgacc gagttcgtac
    14581 tgagaaacag ttcatgcccc gtgtttctaa cacgatgaca atgagtcggg aaatctgacc
    14641 aaggaagctt aatgaaattc accgcggctg cagttcaaat gcttgcctcg gatgacaaga
    14701 cggccaatct ggcggaagcg gcacgatgga ttcgccaatc ggccgccgcc ggcgcgaagc
    14761 tggttgcctt gccggaagtt ttcatctggc gcggggacaa acgaagcgaa cgcgcggctg
    14821 ctgagcccat acccggaccc gcctctaaag ccatggccat actcgcccga gagcttggca
    14881 tctatctgct ggcgggatcc atattggaag aaattcccgg cagcgcaaag gctttcaata
    14941 ccagcctgtt gttcgatcct tccggcaaac tcattgcaac ctaccggaag attcacctat
    15001 tcgacgtgga gttggcgaac aaggtttccg cgcgggaatc cgaaacccgc gcggccggcg
    15061 atacggtggt gatcgccgaa acggagctct gcagcatggg cctgagcgtt tgctacgatc
    15121 ttcgatttgc cgagctctat cgccagctag ctcagcgtca ggctgatctc atcttcgttc
    15181 cgtccgcatt tactgcctac acgggaaaag cgcactggga gccgctcttg cgcgctcgtg
    15241 ctatcgagaa ccaggtttac gtcatcgcgc cgaatcagtt cggcaaaaat ccccggagct
    15301 ttgaaaccca tggtcattcg atgatcgtgg atccatgggg tgagattatc tccgtgatcg
    15361 ccgacggtcc ggggttcatt agcgctgaag tcgatctgga ttatctcagt caggtgcgcc
    15421 gcgagctgcc gtgcctcacg catcgaaagc taagctgaaa gtaacttgat tgcgcgatcg
    15481 gacctacgcg cgcaccgtaa aacattccct tcttatccca cgcacccgat tcaccccacc
    15541 tgcttgctgc gttcggctgc acaggaaata agctctccgc ccgatcttta ggcaacgatc
    15601 atgctccctt ctgtctcttt cccgctgcga tccagggatt gatcaacgta atcaactggg
    15661 cggaaacccg cgttcttcac agttccatgg cattcagctt gctctgggcc ggatgtcaag
    15721 gcaaatcccg cgcttcgtat cttcggggct ggggtttgtc ggcgcggcgg acaatagccc
    15781 ggcgatggga atgcttacac gggtgtctac tcatcattcg atataattgg accgaagtcc
    15841 ctttgtggag ctagttcttc tttcagcttg tgtctgccgg cactgcatga tgattgttcc
    15901 ggcgccctcc acggagagaa tcaagttcgg atcgttcgac attaatctca atagcccatg
    15961 gcagttttgt tgctcgtccg cgaacctgac tgaataactt atgtcgaata catgctgaat
    16021 attaaggagg gttttgatga agaaaggttt actgggactt attcttaccg cagccattgc
    16081 tgctcttgaa ctgccggcgg cactggcgca agacaaaccc ttaggtccgg gctggttgag
    16141 cttggataac aacgtgggcc tgctcgatag aaatatcgcg gagggcaaat ccgctataga
    16201 aggcgctttg gggattggca tcagcggatt tctcgacacc agttaccagt ggagttcgaa
    16261 tcatccgaag aacccgagaa atatttctgg cagatatttc gataaagatt acaacaaact
    16321 agttttcaat tacttccatg tggcagtgga caagcctgaa aaagattggg gcgttggttt
    16381 tcgcctctcc ggcgatttcg gccggggtgg cgagcttctg cgggaggcta cgttgtgggg
    16441 taaaagtttt catgatgagc cttcggctga agtgcgcgaa gcctacctga caacgacgat
    16501 cccgatcggc gcaggtatca gcgtcaaggg cggaattttc gtcactacat tgggcgctga
    16561 aattcttccc aatgctggag cctacaacga caatatttcg agatctttcc tgttcaacta
    16621 tgcgatccct ctgcgccatg ccggggcgct ctttagttat ccgattttga aaaccttatc
    16681 ggtcaacgcc ggcgtggtta caggctggga taatcctcgt gacaataata ggcagccgtc
    16741 gtttgtgggg ggtcttggtt ttacccctac cgagcaattt gccctcgcca gcaacgttat
    16801 ctatggtcca gagaagttca gcaataccgg cgatcagcgt tttaccatga gtcacgtagc
    16861 cacctataag ccgtctgatc ccctagcttt gattcttgag tacacttatg gtcgagaaga
    16921 gaatgcctcc ttgggtggaa cgcgggatgc agtgtggcaa ggaattgccg ggatcgcgtc
    16981 ctacggctgg accgatcgct tcacgacggc gcttcgtggg gaattcttta atgaccggga
    17041 tggtgctcgc ttgggcggta atttttctgg aacgcacgca aacaatagca ttgcggaggc
    17101 gacgctgacc ggctcgtaca aatttaccag gatgttgcta ggtcgggttg aggttcgtca
    17161 agactggtct gacgaagcgt tctttaaaaa aggcgcaacg tcagcggata aaaaccagac
    17221 gaccgttgcg ggccagctaa tatacacgtt ctaggacgat tgcctcctcc tagttcggcg
    17281 gggaatggca tcatgctgtt ccccgttttt tttgagcccc agtcgttcag aatccgacga
    17341 catcctgggg cgatcgcctg cgatatttcc agcacttccg atattgggac tcgctcatgc
    17401 gctctgtaat catctgctgg tgttctcatg gttgttctgt tatcgctcca tggtcgcata
    17461 ggattctcga ttacggcgcc tccggcaccg gatctgattg aggcaaatgc tacacgattg
    17521 cagtcgaggt cgattttaga agacgatgtc acggaataaa atatttgtat ttatcattgg
    17581 ggcagtcttg ttggcgccga tgatctggtg gtcagtccag aaaattttcc aacgagaaaa
    17641 cccgagctcg gaggagcctc ttaaccggct cgctgctgaa atcaatcgtg ctgtccctgt
    17701 tatgatcgac aaagagaccg aattgctggg ttctgccggt gcagagggaa tgctgatcta
    17761 taattaccgg ctggtgagtt attccgtttc ccagttggat gccgccagat tcgctgccgg
    17821 agcaaaagag cgagtctctc aaggcgcttg cagtcgccct gagacccgtg acgacttact
    17881 gaaacaaggc gtgacgctgc gctattcgta tttcgacaaa gacagacaac acatcgccac
    17941 catcgatgtg ttaccggccg actgcggctt ttgatctgcg ggacgcgatc cgtagcgcgg
    18001 tcggctgccc ggccgctaca gatctcacaa atcgctactt catgatctct tttgcctgct
    18061 gtaccagcgc cgcatcgagg tcaaagaccg cttgaacgtt gcgctccagt tcggcgccat
    18121 ccaacgggtt gatatccagt ttcgctttgg tagcgtcggc aatgaattcg gcgtctttca
    18181 ttgtttccat aaacgccctg accaggactt gcactcgatc tttgggagtg ccgggaggca
    18241 gcacataagg tcgcgccgta ggcccgacgc tgtgcaccaa tgcattgatt aatttcttgg
    18301 cttcatcggt tttggcgaaa ttaatcgcca gcggaatttt tggcaggtct ggatgagact
    18361 tcggaatatt ctgtagaact atgttcagtg cgccggaatc aagctctggc cgccaggttg
    18421 ctttgagcga ctcccatgca ttgcagatcc cttgcacttc gccgctgttg aaggccagct
    18481 tcacagtcga agtcccggaa tagcccgata caacctgtat cggcaagcca atcgtttcct
    18541 tgagtacctt ggggatatcg tctgtcgccg agcccacgcc aactccacca aatttgatta
    18601 cggttttcga ggaaagccat tgctcaatgg tggtaaagcc ggtggacttg gaaactgcca
    18661 gaacgtagtt atcctgcgct ggagcgccga cgtagccaaa cttgcgagca tcgaattgga
    18721 tgccttcttt gccaagaagc tgctgtaaga ataagccccc gataaagtga ccgatagtca
    18781 gaccgtccgg cttggcgatg ttatacatgt ggttcgccga tatcagaaat ccggctcccg
    18841 gcatgttctc cacgatgaat gtcggattcc ccgggatgtg ttttcccata tgccgggcta
    18901 tcgttcttga gtacgtatcg taacctccgc cagccgcagc tccgacgacg attcgtacgg
    18961 tcttgccctt atagaatgaa tcctgtgcat gagcgggagc atacgaaagc gttagcaaac
    19021 ccatgatcaa cagcaccggc gatcggacca tatgacctcc cactgatttt cgagttgtct
    19081 ttactgcaag atctctttca actgattcac cagacttgcg tccatcttga acagcgcggc
    19141 gactattttt tcgatttcct caccggtcag cggatcggta tcgagccgcg acttttttgc
    19201 gtcggctaga aactcagggt ctttgagcgt ttcgacgaat gccttgcgca atatctccac
    19261 gcggtcttta ggtgtgccgg ggccgagagc ataaggtctg gtaatcgcgg cgggatcatg
    19321 accaccgtat ttcaaaagct gcttgctatt gtcgttcggc gcataatcga tggcattcgg
    19381 tacgtttggc agctccggat gcggtttagg gttgacttgc aggacgacgg agacattgcc
    19441 ggagtcaagc ccttgacgcc agatcgactt gatcgattcc cattgccagc aaccgccggc
    19501 gacttctcca gcttccgcgg caaggcgaac ttcagccgtg cctttgtaac cggcgaccag
    19561 ttgaatcggc agtttgaccg cggcgatcaa gagctttgca gtattgtaag tcgtatcacc
    19621 cggcgcgaca ccgccaactt tgactggcgt cttggcgttt ctaagggcat cgatagaggt
    19681 aatgccgctg gctttagtga atgcgcaggc gacgttgtcc tgcgtcggaa tgccgatata
    19741 ctcgaatttg cgtgcatcga actcgatacc cggtttgccg atgatctgat tgatgacttg
    19801 attgccgtga aaattcagaa tcgttaatcc gtccggcttg gcgaccttgt agacgtggtt
    19861 agcggctacc agactggcgg cgcccgtcat attctcgacc acaatcgacg gattgcccgg
    19921 tatatgcctg ccgatatgac gtccgatcac acgggagtag gtatcgaaac cgccgccggc
    19981 tgagaaaccc acgatcaacc tgatcgcttt gccttcataa taaggcgccg cggcaccggc
    20041 cggaacggcg agaagcaagc taacaacaag agctgaaaga cccttgccca tgaacttgaa
    20101 cattaaactc tccttttcgg tgaattaatc tgcggcaatt attatgtttt gagctgcgct
    20161 gtcaacgatc gcgcagcatc tccgctctca gttccgcctc ccaagcgcgg ctaccgtcgg
    20221 cttgccactc gcattgtaaa tcgtgaatct ggttgcgatc tgtaaatctc gctagttcag
    20281 cgagcgcggt ttgaagaacg acttcattgc gactgcgatt atgcggtgga ccgaaggtgc
    20341 ggcctaccgg gtgatcgaca aaaacagctc gtggcgctgg cactttgttc attatgtcca
    20401 gggcggtgcc gatcaccagg gttggaatac cgctcgcctc gataacgtgg gcgatgagac
    20461 cgacggtctc atggcaaaac ggtcaataag gcacgagcaa cgcgagatgc acgtccatct
    20521 tttttaattc ggctgcgatc gcgggggcca actcgttttc gatttcttgt gatcgccgaa
    20581 tcccgcccat gaaagagaga tgaaacggag aaacggcgcc gatcacagcg cgctgggcca
    20641 gagaggcaag agcggtgcgt ggaaatacga tctcgggatc ttgtttgggt acccggccga
    20701 ttccggagtg gtggtcgata ataatcgcgc cggcggccga atcggcgggg atgcgtcgat
    20761 aactgagatc ttcgcgcggc acaaaaggtt tctgattttc cagccgcagt gctgcactgg
    20821 ataaaagcgc aaccgttgat tccttgagtg gctttgctac aggcgaccaa gcgtgccggg
    20881 atggtaagac gagaactcgc ggcggcttag gtgcgccggc ttgagccatg atggaaaggt
    20941 aatcgagcga cacagtttac ctccgttcga aggatacttc actccgagat gatctttaac
    21001 tcattggacg gacacaaaaa aattacgcgg gaagcaaaac ctaagggaac gttatgatca
    21061 ccgcgccgca tcggtcttat acgcggcgac ctttcggttg aaaccaatcc cccttgcgca
    21121 aatcgagccc cgtgtgtcct tgaggtatca cgacactgca gcgacattca aatctgagac
    21181 aacggaagtt tgcatgtgtc acatattgaa caatctaaag aaccccactg ctggcgaact
    21241 atggccttgc tcttgcgttg cagttgacac ataggaggga acaacaatgg atgcactcga
    21301 actcttgaaa caggaccacg ccaaagtcaa ggaactgctg gagaaggcgg agagcgccga
    21361 acagggcaaa gaacgaagtg ccattttcga aaagatcaaa actgagttag aaactcatgc
    21421 gcggatcgaa gaaaacgttt tttatcccgc ggtgcagaag aacgaagagc tgaaagacat
    21481 ggttctggaa tcaattgagg agcataaaca agttaagacc ttgctgcgag agatcgatag
    21541 cctttcgata gacagtgaga aatttgagcc gaagctcaag gtgctcaagg aaaacgtcga
    21601 gcatcatgcg gaggaagagg aggaaggaaa gatgtttccg aaagtccgca agctcttcag
    21661 cgcaaacgag ctacaggaac tcggagcgga actggaaaca gcaaaaggaa aacgggagcg
    21721 caaggcaagc taaagcaagc ggcatgcagt gattacagta gatgaggcaa gcataatgga
    21781 aacctatgat gtaacaagcc agacaaatgg gtcccaagcc gtgatcgata gtgaggatcg
    21841 gttcacgaag agtatcgagt cttataccgc ggctataccc tcgtcggcct atctcggcgt
    21901 ggccgtcgga gccatggcac tttccttgtt ctgccagatt accggtcgcg gcaagtgggg
    21961 caatttcatc gcgcagtggg tgcccacctg gttgatcatc ggagtttata acaaaatggt
    22021 taaacttggg ggccacgacc agtttgatcg cgggatcatg gctgaaccaa tgggcgatta
    22081 cacttgcgag ttctgtgata gcaaatttag tttacgctca gatctcgaaa atcatcgaaa
    22141 gcattgcagt gtgcgcactc cggtcagtta attcttggta ggtgaaatct gcaaaaatcc
    22201 attttacgct cctctatgca ggggtgcgtg ttcgattgat aagcccaata ctccgagggc
    22261 gcttcctttg tcagttcaga tcgtctgtct cgagaagact ccggctgctc actcacgcgt
    22321 cacatcagct ttagcaatta gccagcgacc attccgcttg atgccttgaa attcatagct
    22381 gcccgcggag ctcgcattaa accccagcac cttaatgccg tccaactgat agctgccttt
    22441 aaccaccgca ttatcgccat cggtcgaaac tccctcgata tcattcgtaa gtgttgtctc
    22501 tttcagcatg ccgctttggt gtttggcgat ggtgttcttg atttcttcac gcgatttaat
    22561 ttccgagccg gtagggagac gcaaaacacc gtcaggcaaa aacagttgag ctattttatt
    22621 ggcatccccc tgattccatg cctgtaccag ttgttcaacg gtagattcaa gaccagcgtc
    22681 ggtgttggcg tcagatttcg gtgcttgcgg agctacatct ttcgcttgct ccgtgacttc
    22741 gagcggttta tccaatgatc tatcggactg cacggcttct ttctttgcgg gacgttcgcc
    22801 gatttgctgt gtaacaacga tggcgaaaac cagtaagaca gcggccgata acgcaagcgc
    22861 tgcccacgat ttgtgagaaa tttgtgcggc tgaagtcatc ccagtttcct gcacgcaaat
    22921 gcagtgcccg attttcttag aaggaattgg atctcggtgc gttatgttgc agtagatgaa
    22981 ttcgacgctc tcggttcgcc agcagcttgt cgaactaaat ccctctatga tcagcgctat
    23041 ttctcactgt agtatcgccg gagaggctga tagtttagtc tcatggaaat ccagttattg
    23101 aagcttagtg tggacgacgc cgatctaaat gaaatggcac ctcaagtctt cgtctggccc
    23161 gacgctatcc gcgacatcca tttcgcggtg aggccggaag gcgttagggt tacgggcgtt
    23221 tatcaacaat tcgtcgggat tccatttcag atgttgtggc aaatatcagt ttcccacggc
    23281 atgctcgtcg cccgtatcga acgtgtgaca gccgggtttt ttaacgtcgg gtttatcaaa
    23341 aactatcttc tgaaatttat tgccgctgcg acaaattcag tgattcgtga cgggatgctc
    23401 ctatttgata ttgatgcctt cattgcggat aagggctggc cggtgcgact caactttatt
    23461 tcagttcgct ctactctcgg tagtttggtt ttagagagcc gagcacgtgg tgagatcgac
    23521 gaacctgcaa gccaatgacg ctccggtgac ctatggtgtc gcactccggc gatacggcag
    23581 cgaatgtaac gaacttgtct gtaacggcta cttttcgcac tactatcgcc accaccggag
    23641 tgatttacaa aagtggatat gataagcaat ctccactatg gtggatgtgg cggcgaacta
    23701 tcgagccatc gttgaacggg tcaggcaggc agcggtttcg gccggacgaa atccgcgtga
    23761 gattaaaata cttgcagccg cgaaagcaca agacattaaa tcaatcgaag ctgcgattat
    23821 gtccggtgtg ccgctcatcg gtgaaaatta cgttcaggag gcaagcctca aaaggaaaag
    23881 tcttcccacc agtgttgaat ggcacatgat cggccacttg cagcgcaaca aggcaaaagc
    23941 ggcagtggaa atcttcgatg tcatcgagtc tctcgataat gccgaattag cgcaagagct
    24001 ggataaggaa ggccgaaaac gaaatattca agtgcgggcg ctcatcgaga tcaatatcgg
    24061 cggtgaagaa acgaagtccg gggttgccaa gaccaaagtc gcggagcttc tacgcctaat
    24121 gggccagctc agtcatctca gcatccaagg gctcatgact gttccacctt ttcgcgagaa
    24181 ctctgaggag actcgacctt actttcgtga gctgcgccag ttgcgcgatg aactcgacag
    24241 cttgcagttg ccgaacgtcg atcttaggga gctatcgatg ggtatgacgc atgattatct
    24301 aattgctgtt gaagaaggcg ccactattgt gcgaattggc acagccctat ttggtccgag
    24361 acgaacctag ccggaaaatg tttattcttg ctaattttat cattgccacg gctcaggtgt
    24421 tggacttctt gctctgggct taccaatgga ttttgattgc gcgagtggtg atatcctgga
    24481 ttgacgcgga tccgtataat cccatagtac gattcattta taatttgaca gagccggtgc
    24541 tcgagaaggt tcgtgagagg ctacctctaa ttgccggcgg attcgatctt tcgcccttgg
    24601 ttgtttggct cgccatctgg tttatcaagc gatttctggt tcaatcactt tatgaccttg
    24661 cttattcatt gaggtaatcg ccgggttgcg atcatcaagg tctcttcacg accaatcttg
    24721 gcggggacgg gcagctccta ccgtggcgct gcctaacaat gattgagtga caggcaaacc
    24781 gaagacaaag aaaaatagtt cggcccgggt atttagttaa ggagattgca ctcatgaaaa
    24841 ttttcttaga aggccgttgg attgataagg ccaacaagat cgaagtcaag aaccctttcg
    24901 ataattccgt catcgacacg gttcccaaag cggatgggag cgatgtcgaa aaggcgctgg
    24961 cttacgccga acgtggagcc aaggtgatgg cccggcttac cagctatgag cgctggaaaa
    25021 tcttacgcac cacggcagat ctgatggccg cacgcaacga agagctcggg cagaccatct
    25081 ctaaagaaga aggaaaaatc attgcggagg gtcgaggaga agcaagccga gcggtggaaa
    25141 ccatgatggg ctcggctgag gaagccaaac gcctgcacgg ggaaaccgtg ccgctggatg
    25201 cggatcctac cggggacaag aagttaggat ttacattgcg cgtgccctgt ggtgtcgttg
    25261 cagctatcgc gccgtttaat tttccgttga atctcgtctg tcacaaagtg ggtccggcgc
    25321 tcgcagcggg aaattcggtg atcgttaaac ccgcttcgga tacgccgctc tcggcgttga
    25381 agctcacgga aatgcttctc gaagccggcc tcccgccgga aggaattcaa tgcattacag
    25441 gttcgggcgg tgaaatcggt gacactctgg taaaggaccg ccgcgtgcga aaaatcacat
    25501 tcaccggcag ccgcgaaatt ggcgatcgaa tttgccgcat ggccggaatc aaaaaagtca
    25561 ctatggagct tggctccaac agcccgctga ttatcatgcc tgatgccgat ttaaacaggg
    25621 tggcggcggc ggtagcgacg acagggtatg gcaatgccgg gcaaacctgc atttcgactc
    25681 aaagagtact tacagccaag aaggtttatg gtgatttcct cgatgctttg aaacccaaag
    25741 tcgaagctct cagcaccggc agccaactcg atgaaaaatc aaaagtcggg ccgatggtaa
    25801 aggagagcga ggcggtccgc gttgaagact ggatcaatga agcggtcgcc ggcggagcac
    25861 gattagcggt cggtggcggc cggcgcggtg caatctacac gccggcagtg gtggcagatg
    25921 ttactccgga tatgcgtatc tcgcgcgatg aactgttcgg cccggctgtg gccgtgactt
    25981 cgttcgaaaa catcgaagaa gccattgcgc ttgccaatga tagcatttac ggcctggcag
    26041 cgggcatctt taccgagaat gtggaatggg ccatcaaatt tgctcgtgaa gtcgaagccg
    26101 gcaatctgca cgtcaactgg ggaccgcaat ggcgcgtgga tctgatgcca tacggcgggc
    26161 ttaaggattc aggctttggc aaagaagggc cgaaatacgc ggtcgaggag atgaccgagc
    26221 tcaagatggt cgtatttcac ctgaatagtt gatcatctgg gacaaccttt cagagcggac
    26281 gaacatcaaa tttggacaac tcgcaagcgt tggaaccaat gatgagaagc gacgggcgaa
    26341 aaccaaaaca actgcggctc ataaaaatag cgccgaacta tctaaagact gccgatggtt
    26401 cggtactgat cgagatgggc gatacgaaag taatttgcac tgcgaagctg gaagaccgcg
    26461 tgccgccttt cttgcgcaat agcggcaagg gttggatcac cgcggaatac ggcatgttgc
    26521 cgggctcatc gcaggcgcgt atcggccgcg aatcctcccg cggcaaagtt ggtggcagaa
    26581 cccacgagat ccaacggttg attggccgaa gtttgcgggc gatcacggat ttgacccatc
    26641 taggtgagcg cacggtttgg atcgattgcg atgtgatcca ggcggatggc ggcacccgca
    26701 ccgcttcgat taccggcgct tatgtttctc tgtgccaagc gattcgcagt tggcagaagc
    26761 gtggcatcat cgcgcaggag ctgatcaaag attccgtcgc cgcggtgagt atcggaatta
    26821 tcaatggcaa gataatgttg gatctatgtt acgaggaaga cagcaaagcg gatgtcgata
    26881 tgaatttcgt tatgactgga tccggtagat tcgtcgaaat tcaagggact gcggagagcg
    26941 cccccttcac caaaaaacaa atggacaaga tgtcggaaat cgctgaacag ggaatcagag
    27001 aattgcttaa ggcccagaaa caagctctcg cgttcacgtc ctgaatcctt tatttcgatg
    27061 atcgatctgc ttgtcgcgac caccaacagc ggaaaattag cggagatcaa atcgtttcta
    27121 aaatatctgc cggttcatat tatttcatta agcgacctac aagatccgcc ggtggttgct
    27181 gaagatggcc agtcgttcga agagaatgct ctcaaaaagg cgcgaacgct tgccgaatgg
    27241 tctggctacc gcacattggc cgatgattca gggttggaag tggatgctct cgacggtagg
    27301 cctgggattc tttcggcgcg atttagcggc attgagggtg atgatgctgc gaataatagc
    27361 aagctcctcg aagagatgag ggacgtgccg gaggaaaagc gaaccgcgcg gttcgtttgc
    27421 gtgttggcgt tgtgcgcccc gacatcggaa ggcggcacgc agtgcacctc gcggggtagt
    27481 tgcgaagggt taatcgggtt tgccgcgaaa ggcaagaatg gcttcggtta tgatccgtta
    27541 tttttcttcc ttccattaaa caaaacattc ggcgaaatcg atcagcagac caagacaacc
    27601 gtcagtcatc gcggcaaggc gctcaaagaa ctcgtaaagg ttctgccggc atggctcgat
    27661 gtcgttaaaa aaccttgatt tagttcttta gccgtgtagt attgattctg ttcgcttgac
    27721 ggggcgtagc gcagcctggt agcgcacctg ccttgggcgc agggggtcgg aagttcaaat
    27781 cttctcgccc cgaccatcaa gactgctgag taatgagtgt tgagttcagg gtagctcacc
    27841 gcttattact cgctgttgtt tttggcgcca gtagctcagt tggatagagc aacggccttc
    27901 taagccgtag gtcaggggtt cgaatccctt ctggcgcgcc agtatgggat catatggtga
    27961 gtgtagctca acggttagag cactggactg tggctccagg ggttgagggt tcaaatccct
    28021 tcactcaccc cattattctc atccgtaaaa ggatcggagc cggcaagtcg tttcgacatt
    28081 atagatagtt tgggccgcta gctcagttgg tagagcagct gactcttaat cagcgggtcc
    28141 ggagttcgag tctccggcgg cccaccaaac ttcttctaac ctttccgaca tcggaagtca
    28201 ggcgtcctga agagtagcca tctgactcat tcaaactcgc tgcgccagga gttaattctc
    28261 tctccttctt gtgtttatcc agcttgccgt gctgcatatc tcgcgacatt actaagcgcc
    28321 aaaaatcaat tgttaagggt tgtccgtagt gagataatga atatcatttc ggtttggcat
    28381 acgcgaaagt cctaggccgc aaacagggga aggaatgtta tggaactgac ggcaacgacg
    28441 tattccatga ctttggaggt ctacttgcaa tccgcggttc tgcgtggcgt gctagttacg
    28501 aatcaggatc gtttaagtaa ttacctgata cttcgcgaag gagatgaagt tctatcaatc
    28561 acggatgcca ctcttgacga tttgcagggg aaagcgatgg cgacgagctc tgccgaatat
    28621 ttgatttata tgcaggaagt atttttaatc ggtgacttga gcccgcaagt cagctcagat
    28681 cgggccggct acgaacatct ttacgtcaaa aaggaatcga acaaagcctt gattagtgtg
    28741 ggaccgtact ggttgagagg tgacattcat gtcataccgg gcgctccgct gcatgagctg
    28801 ctcttaggca agacgcgttt cttcccggtt acggatgcca tgattatcgg caggcccgat
    28861 gtcgcgcccc gaacgtactt agtgaatcgc acaaagattg gttgcatgac ggcggtgggt
    28921 gacggtctgg ttgaacttta atgctggagg caagcgaacc ctgcatgact aggcgatttc
    28981 caaccgagat caatcacatc aaagcagagg gcgttgtcag gattctgtgg gatgatgacc
    29041 acgcgggaat ctatccagaa gcgtacctgc gcggatattg tccttgcgcc gtctgccagg
    29101 ggcacggcgc cagccggaag tttgtctccg tcccggatgc taagttgatt gacattcggc
    29161 cgatagggaa ctacgcgata gagtttcact ggcacgatgg tcacagtacc ggcatctaca
    29221 cgtatgatta tcttcggtct ctctgtccgt gttcggagtg cgcctcttcc gtcgatccca
    29281 gtcaataagc aatgctccgt tgtttggctc cggctaatcg tcttcaaaac ccctgctgca
    29341 gtaaacgaac cggatctccc cgcccgccgc cgatccgtcc tagaataaag cccacaagca
    29401 gttcaacggc aaccaaatcg tgcttgtcgc gcgcgcaacg cttgacctaa atagacattg
    29461 ccaatacttg acttagtgga acgcctttcc tactctgttt tgctggtcca gagagaggaa
    29521 gctgcttgat cggtaatttg ttttctacag gcgagagtgg cggaactggc agacgcacta
    29581 gacttaggat ctagcggtaa caccatgggg gttcgagtcc cccctctcgc accagatttt
    29641 gtcgtgagat aaggaaggga atttgtggtg atcatgtcca taccaaccgt aatagaaagc
    29701 tgcggatcat cgctttagag ctatgaagat agatatcgct gagctaagcc ccgtacaacg
    29761 taaggtgcag atcgagctgc cggcagaaga agttgccggt gaattctctc gcgcctacca
    29821 ggaccttggc agacgtgttc gcatcaaagg atttcgcgcg ggaaaggctc cacgaaatgt
    29881 tctgcaggga atctatggag aagaagtaag agagcaagtc cgttcacagc tcgtcgaaca
    29941 ttccctcgga gaagtaatca aggatcgagg cttgcaattg atttcccgcc cggagatcga
    30001 ggcagccgaa ctgcaagaaa acggagagtt ttcgttctca gctgttttcg aagtcaagcc
    30061 tgaaattgat gttgagaact accttggcat cgagctcgat aaggttcgag tgagtgtgac
    30121 aggcgatcag gttggtgaag cactaaaacg gttgcagcag agccacgctc gcttggagcc
    30181 ggttaccgat cgcaacatcg ctcagcgggg ggatttcatc acagtagatt ttgccggatc
    30241 tgcggatggc aagccttttt ccggcggcaa aggcgaaaac tacgtcatcg aaattggggc
    30301 cggacaggca ttaccgcaat ttgatgatgc tttgcaagga gccaagctgg gcgaggaaaa
    30361 gcaaatcaac gtcacttatc cggaagacta tcccaatcgc ggacttgccg gtaaaactgc
    30421 cgagttctca gtcaccgtcc gtgagatcaa gcagaaagtt ctgccgacga ttgatgacga
    30481 tttcgccaag gatcatggtg aatgctcctc gttagaggaa ttgaagggtg ttatccgtca
    30541 acggctcgaa gcggagctca cgcaatatca acgagaagaa ctcaaagagc aacttctaag
    30601 tcatctgatt gagagcaatc cgtttgagcc gccaacctcg atggtccagc gtcagactcg
    30661 atatctttta gaacgaaacc caacatcgaa tgcgaactcg ccagaagcgg aaacagctcc
    30721 aaccacggaa gaaaatagaa aggctctcga ggctcgtgcc gttcgccagg tgcaagcgac
    30781 attgttggta gagaagattt cacagttaga gaaaatagat gtcgaagatc gtgacgttca
    30841 agagagaatt gataagctcg tcagggctgc cggcgaacgg ggcaaggctc tgcgcgattt
    30901 ctattccaga gccgaggcgc gcgccgatct acgaacgcaa atagcatttg aacgcacgct
    30961 ggatttcttg ctgcaacgag cggcaattaa agagatagat cctcctttaa ataaggttga
    31021 tgacgaaaat aaaaaaagtt agaatgctct cgtagttaag gagtttgcta aagatgaatt
    31081 ttattccgat ggtcgtcgag caaaccggcc gcggggagag agcgtatgat atttattccc
    31141 gcctgcttaa agatcgcata gtgtttttag ggtccgttgt gaccgacgat gttgctaatc
    31201 tgattaccgc tcagttcctg tttcttgaat cggaagatcc tgaacgcgat attttctttt
    31261 atatcaattc accgggcggt tccgtcactg caggtttagc gatctatgat acgatgcagt
    31321 atgtgcgacc acagatctct accgtttgtg tagggcaggc tgctagtatg ggcgccgtgc
    31381 tccttgccgc tggcagtaag accaaacgct atgcgctgcc gcatgcacgt attatgattc
    31441 accagccgct tggcgggttt caaggtcaag cggccgatat cgatattcag gcgcgtgaga
    31501 ttcttagaat gcgtgaagag ttgaataaca tcctcatgaa gcacaccggg caaagtttga
    31561 agaagattga gaaggacacc gaccgggaca tgtttatgtc aggtaaacag gcgcaggaat
    31621 atggtctggt tgacgaggtg atcgcggctc gacctgaagg ccttttgaaa aagtgattca
    31681 aacgaagcgg gggttctatg ccaaagcatg gggatgatcg atccgggaat ctcgtttgct
    31741 ctttctgcgg aaagagtcaa gacgaggttc gtaagttgat tgcgggacca accgtgtata
    31801 tctgtgacga atgcatcgat ctatgcaacg acatcattgc cgaggagtgc gatcacgacg
    31861 aaactcttac ggcttcgtcc aatgttccaa agccggctga aatcaagaga gttctagatc
    31921 agtatgttat tggccaggaa cgagctaaaa gaatcctcgc cgttgccgtt cataaccatt
    31981 acaaacgtat tgacagtcag atggtgaccg gcgatgttga attgcagaaa agtaatattc
    32041 tactcctggg gccgacgggg tctggaaaga cactcctggc acagactctt gcacggtttc
    32101 tgcaggtgcc tttcaccata gcggatgcaa ccagtctcac tgaggcggga tacgttggcg
    32161 aagatgtaga aaatatcatt ctgaaccttc tgcaagctgc ggattacgac gttgaaaagg
    32221 ctcagcgggg tattgtgtac attgatgaga tcgataagat ttctcgaaaa tcggacaatc
    32281 catcgattac ccgcgatgtc tccggggagg gcgtgcaaca ggctttgctg aagattattg
    32341 aaggaaccat ggcgagcgtt ccgccaaaag gtggccgaaa gcaccctcag caggagttct
    32401 tgcaggtaga tacgacgaat atcctattta tctgtggtgg tgcttttgtc ggtcttgaag
    32461 atattatccg caagcgaacc ggaaagaaac gcatgggctt cggtggacat ctcgacgagc
    32521 aagatggcaa acgccccacc gagcttctgc acgaaacgag acccgaggat ctgctcaagt
    32581 tcggattgat tccagaattt atcgggcgat tgcctgtgat cgctaccttg gacgaactgg
    32641 acgaattcgc gttagttcgt atactaaagg aaccaaagaa cgctctgaca aaacagtatt
    32701 ccaagctttt cgagatggaa agcgtgcatc tgaagttcac tgaagaggcg cttcggtcga
    32761 ttgcgcgtga cgcgatgaaa cgaaaatccg gcgcgcgcgg gctcagagcc atcatggaaa
    32821 atatcatgct agatatcatg tatgacatgc cctcgcagcc caacatcaag gaggtcgtga
    32881 tttccgagga agtggtcaac aaaaacgaac ctcctattgt tgtgtacacc aaagcagcgg
    32941 agagcgctta ggaagggaaa ccgtatgttg tttcgcagtg acaaaaaaga tgagaaggaa
    33001 aaagagtcgg gtggctccac ccgtgtcccg ttgttgcctt tgagggacat tatcgtcttc
    33061 ccgcatatgg tcgtgccgct gttcgtcggc cgtcaacgtt ctattcgagc actggaagag
    33121 gctacccaaa agcagggacc ggtcttcttg tcgtcacaga aggatgccaa gaccaacgag
    33181 ccggccgagg atgatatcta taaaatcggt acgttgggca ccgtggttca gatgctgaaa
    33241 ctcccagacg ggacggtcaa agttttgatc gaaggaaaaa ggcgcgctca aatcgctcgc
    33301 tttgtaaaca atcccgaatt ctttttggta gacgtggacg aagctccgga agtcaatgaa
    33361 cgcaataccg aagtagaagc tttaacccgc gaggttcata caacatttga gaattacgtt
    33421 aagctgaaga aaaaaattcc tcccgaaatg gtcatgtcgg tgtcttccat cgatgatccg
    33481 ggacggctag ctgacacaat cgtcgctcac ttgggaatca agttagaaga ccggcaaaat
    33541 ttgcttgaaa ccttcaatgc ggccgaacga ctggagaagg ttttaggcca tatgcgcgcg
    33601 gagatcgaaa tactagaggt cgagcgccgt attcgttcgc gtgtcaagaa acagatggag
    33661 cgttcgcaga aggaatacta cctcaacgag caaatgcgcg cgatacaaaa agaacttggt
    33721 gaaaaggacg agttcaaaaa cgaaatccaa gaaatcgaag agaagatcaa gcaaaagaag
    33781 ctgtcgtcgg aagctcgaga gaagatcgac aaagagctta aaaagctaaa gatgatgtcg
    33841 ccgatgtccg ccgaagcgac agttgtcaga aactacatcg attggatctt gagcctaccg
    33901 tggaatgaat ttactgatga taagcttgac atcactgaag ccgaacgggt tttggaagaa
    33961 gatcattatg ggctcgagaa ggtaaaagag agaattctag aatatctggc ggtgcagagt
    34021 cttgtgggta agatcaaggg tcccattttg tgtctcgttg ggcctcccgg tgtcggaaaa
    34081 acttctttgg gtcgatcgat cgcacgcgca accgggagaa aattcgttag agtatctctc
    34141 ggtggcgtgc gggacgaggc cgaaattcgc gggcatcgcc gcacatacat tggcgccttg
    34201 cccggaaaaa tcgtccagtc gatgaaaaag gcggggtcga gcaatcctgt gtttctgatg
    34261 gatgagatcg acaagatgtc aactgatttt cgaggcgatc cttcatccgc attgctcgag
    34321 gtcttagatc ccgaacagaa tacggcattt aacgatcact acctcgactt ggattatgac
    34381 ttgtccaaag tcatgtttat taccactgca aatacacttg atcgtattcc ccggccacta
    34441 caggaccgta tggagattat tcgaatcgcc ggttataccg aactcgagaa gctcagtatc
    34501 gcgaagcgat atcttttgga gaagcagaag gaagccaatg gactcactcc cgaaaacgtc
    34561 acatttaccg ataatgcttt gttgggagtg attcggcact acaccaaaga agccggggta
    34621 agaagcctcg aacgagagat cgcagcgatc tgccgaaagg ttgccgttga ggtggtcaag
    34681 aaagatcgta acacccatat gcaggtggga accaagagcc tccataaata tttggggccc
    34741 gtaaaatacc gctacggcaa ggctgaagac gagcagaaga ttggcgtaac aactggcttg
    34801 gcatggaccg aacttggcgg tgaattgctg gcaactgaag tgaccgttat gccgggtaaa
    34861 ggacagctga tcattaccgg aaaattgggc gatgtcatgc aagaatccgc gcaagccgcc
    34921 atgagttatg ttcggtcgcg agcagcggag cttggcttgg acaaagactt ctatcaaaag
    34981 atcgatgttc acatccacgt tccggaagga gctatcccca aagatgggcc ttctgccgga
    35041 ataacgatgg cgacatcgct ggtatcggca ttaacgacgg tggcagtgcg caatgatctc
    35101 gccatgaccg gtgaaattac tctgcgtggc accgtccttc cgattggcgg gctcaaggaa
    35161 aaggtattgg ctgcgcatcg cgcggggatt aagaaagtgt taatacctgc cgagaatgag
    35221 aaagacatag aggaaattcc cgccgcggtg ttaaagactg tggaaattga attggtcgcg
    35281 cacatggatg acgtgctgaa gaaggcatta gtcattgaca atccagaaaa tctgttcaaa
    35341 cgcccatcgt tatctattga tgcgaaagag ggcccaactc cgttcgggga aaagatcgac
    35401 gataacgctc ccgctgagat ccttcctcaa taactattcg gacacttcgg ccagcccgtc
    35461 gtgatttgac acgcggctgg ccgactgtta taaacccgca acgttttgac tagacagatt
    35521 attgaatgga agagtaatga ccaaggcaga cttaattgat tcattagcaa gacgcgctga
    35581 aatgccccgc caaaaagcag aagaaattgt caatggattt cttgatgata ttgttgcagc
    35641 tttgaagaat ggggataaaa tcaatgtttc tgggttcgga actttttcga tctcggagcg
    35701 taaaggccgg accgggagaa acccgaagac cggggaagca attgaaattg ctccttctag
    35761 agtcgctaag ttcaaggctg gtaagctcct aaaagaggcc ctcagctaaa acaccctgtc
    35821 gcgcatttaa atccaagctg gtggatacac cagagatagt acgcaagagg ggcggttagc
    35881 tcagcgggag agcatcggcc ttacaagccg ggggccacag gttcaaatcc tgtaccgccc
    35941 accagtcgtc gttaatcaca tcggggtcgt agttcagttg gttagaacgc cggcctgtca
    36001 cgccggaggt cgcgagttcg agtctcgtcg gccccgccat tttgtgtgaa gtggcataga
    36061 atatttgttg gggatagcga gtatgaatac cgaagaagcg cttgcacaac gagagtggta
    36121 catcgtagat gcggatggca aaatcttggg ccgcatggcc agcgagattg cgaaaattct
    36181 acgtggtaag aacaaggtgg ttttcactcc caatcaggac acgggcgact ttgtcattgt
    36241 tatcaatgcc aagggtgtga gactttcagg acacaaactg gatgacaaga tgtattatcg
    36301 tcatagcaac tatcctggag gaattcgttc acgtagcgcc gcgaatcttc tcgatgagaa
    36361 accggaagag ctgattcact tggcggttaa aggaatgttg cctaagaacc ggttagggag
    36421 aaaattagcc gcgaagctca aagtatatgc cggcgccgaa catcctcacc aggcacaaaa
    36481 accaaaacca ctgacgatcg gggcgtagga ggctctcaaa tggccgaaaa agtaatcacc
    36541 gcaacgggga gaagaaaaac ttctgtagct cgcgtgtttt tacggcaagg aaagggcgcc
    36601 atcgctatca atgatcgaac tctcaacgag tattttggtc gcgagactgc tcggatgatt
    36661 gtcatgcagc cttttgagtt gacccagacc acaggaaatt atgacatcga agtcaatgtt
    36721 aacggcgggg gcagttcagg gcaagcaggg gcgatccgcc atgggattac gcgagcgctt
    36781 atgttcctca atccggattt tagatcgtct ttgaaaaaag ctggctttgt cacccgtgac
    36841 ccgcggggtg tcgagcgcaa aaaatacggc cggcataagg cgcgaaaacg tccccagtac
    36901 tcaaagcgat aggccggatg cagagagttg ttgaaccgtt ctgcacgatt tcgtttgcgt
    36961 cagtaatcgt ttgaacttca catcaggttg ggccatcaac gccgattctt ctaggactct
    37021 tccgctgtaa acagataaat tcccaggagt gcggagatac cattgggtaa ccacgcggcg
    37081 atcgtaggtg gcaaggcgcc gctatgtccc agagaaaagc agaacgcgga aagcacccaa
    37141 taggcgaagc cgatcagcat cgcgatgccg aaactcagcg ctaacccgct gccaatccgt
    37201 cttttcaagg caagaggaat cgcaacaagg accatcaggg gcgaaataaa tggaacggcc
    37261 agcttcacct gcaaatcgac ctgataagct gtggcatcca tgcctttact cttcatttct
    37321 acgatttgtt tttgcagatc gaaaaacgta aattcttcgg gatcgcgagc aagtaacatg
    37381 aggtcgtcgg gggtttcaga gatcgtaggt ggcgtgctca cgattcgttg ccttagcttt
    37441 ccgtctggta aaacttgcca ttcgataccg ctttctgatt cccatccttg tcccagccat
    37501 tgtgctttgg gaactccaat cgtagactgc aacgtaaaat cgcggcttag cacgaagaat
    37561 gtaatcccct ctagagcatt cttgtttgga tcgaaattgt ctatgttaac gaagcctcct
    37621 tccgcacgga tccagatatc tttggttccg aataagcttt gttgctgtgt cttcttaatt
    37681 tcagtcttat aaatagtctc ggcacgatgc gcgaacgccg gtaccagtcc ttcattccaa
    37741 agaaaattcg tcatgcaaat cagcaatgag aggcatagca gcgggaatga caggcgtcga
    37801 gtgctcagtc cgccggactg cattgctgta atctcctgcg ctttagaaag cattcctagg
    37861 cagaagagag tggaaaacaa tgtggcaaaa cctatcaccc gagatatcga tagtggaatc
    37921 ttgtacaaca cgtagcgaag tgcggtggta acggagattc cagattccac cagagtgccg
    37981 atccgttcaa aaaactcgac gatggcgaag attgcagtaa aacagagcaa gctggcgata
    38041 aaaacccgga caaagctgct ggcgatgtag cgatcgagta tgctgccaaa cggtagaagg
    38101 gtaaatcgcg gcttttgttg cataagcgca attaacggtt ttgaaatctt gtcagtctct
    38161 gaccgagcca tagagatgcg gcatcaaacc tggtctgcaa tagcagtggc gattcataga
    38221 gcgctttccg gaatagatgc aatgaaatca caccgactac aatgttcgga aaccacacgg
    38281 caatgaacgg atgaaaagta cccttctctc ccaatgcctt tccaaacgaa agcagggcgt
    38341 agtagacgac aagccagacg atgcataaag cgactcccca cgatctattg gcccgtgacg
    38401 tccgcggtag aagcgagaga gacacaccga gcaggcagaa aaccagaggc gcaaaagaga
    38461 acgcaatccg ctggtgaaac tccatcaatt caggtctggc gatgatgccc gcagcctgct
    38521 tttcgtcgat tgtttgaagt aggcggctta gagacatctc cttgggtccg gcatctttca
    38581 tttttgccgg cccgatgaac tcgtctaaat ccaacttgaa atcatagatg ttgaagttcg
    38641 tctggctgaa atctgagcgt gttctatctc gttcataaat cgatccatca aacaatctca
    38701 atgagagggt atgtgttcgc tcttcggtgc cgatcaggcc gaccttgccc agaataatgt
    38761 tgtccttcga cttgtcgcgt ttgtcgacaa ttagaatgcc ttgcgcaaca tttccgggag
    38821 gtattatctc ttcaacatag attagtatat ttggaaagtc ctcgttgaat accttctctt
    38881 tgagagcagt gccgatgcga gtcctagcga tttcgtaaag ttcgttcttg agagccactt
    38941 gggcggcggg tcgcaccgaa ctcgtcagga gcagggttat caccgcgaca ttcgccgcca
    39001 caaaagtgag agggataaga atctgtttcg gactgactcc ggaggctttt agggcgagaa
    39061 gttcctgatc gcctgatagt ctgccgagcc ccaagagtat ggccaaaaga aatgccatcg
    39121 gaagcgttaa ttccaagaaa gtcggcagaa tcaggacaaa taatcttccc acttgggtga
    39181 ggggtatgcc gcgggtgacg ataagctcga cgagctttaa aattcttgcg attagaagga
    39241 tgaaagtgaa agtgagtagc ccaaggaaaa aaggcgggaa gatttccgag agaagataac
    39301 aagtaagagt cctgcgcttc acaatgtttt tatggtagcg ggtggttctt ttgttgtaaa
    39361 tggttcgccc attctcgcga gaacatatgg aaaatgaatt tattatcggc atccatccga
    39421 ttctagaaaa gctcaaggca tcccctgaag agatctctgt tatcttactt gctcgcggcc
    39481 cgctacgacc aactcttaag tcattagaaa gcgaggcgca gaagcttcag atcaagatac
    39541 agttcatgga cgccaaagag cttgattatc tggcgctggg gcaaagtcat caaggagctt
    39601 tagcgcgagt ccggccatat tcgtacttgc ctttgtcgga attggaaaga accctcgctt
    39661 cggaagcatc tccccagaga atcgtcatat tggacggcat tacagatccg agaaatttcg
    39721 gagcgattct acgcaccgcc gaagccgtcg cggtcggtca cattattatc cctaaggacc
    39781 ggtcggtgcg cataacgcca atagtactca gaacctccgc cggagctgtt aatcacttgc
    39841 ggatctatcg agtgaccaat ctgcggcgcg ccatagcatc actcaaaaaa tcaggatttt
    39901 ggatcgcggg tttagatgcg aatgccagga caagctatga gactataaaa tatccggaca
    39961 aactcggcat cgtcctaggc agcgagggtc ggggtcttcg tcctttgatc tcctatgaat
    40021 gcgactatct tgtacgtatt cccatgaaag gcaggattac gagtctcaat gtttcggttg
    40081 ccgcagcaat attattgtat gaggtttcgc gtcagcacgg cgacattgac aagggtaaac
    40141 ctaacggata ttagggggtt gtcgaggagg gcgtctggcc agcctgttga catcataggt
    40201 cgaagatggt ttaaatatcc gtttgcggaa gtggtatgac ctctttgccg tcgttgtccg
    40261 ttgcgtttca agaacttact actaatattg ttaactaaat tgagctgaag cggtcattgc
    40321 cgttgtgatg tacccgttgg tgctcatatt gttggtatca acccattgtg atgctggcgt
    40381 agctcaacgg cagagcagct gatttgtaat cagcaggttg taggttcaag tcctatcgcc
    40441 agctccagcc aagcgaacct ggcaacgagg gcatcggtgg tggtgagtcg cagtgggact
    40501 tcagcaaaca tattgctgat agcggagagg tacccgagtg gccaaaggga gcagactgta
    40561 aatctgccgg cgatgccttc gaaggttcga atccttccct ctccaccatt tttcgcggaa
    40621 atagctcagt tcgcgggaat agctcagttg gtagagcgcg agccttccaa gctcggggtc
    40681 gcgggttcga gtcccgtttc ccgctccact tgcgtatggt tgggtgctaa aagcagatgg
    40741 ctcgattata cagttcagcc atcgagatca gccatcgact gatacgccca ggtagctcag
    40801 tcggtagagc acgtccttgg taaggacgag gtcagcggtt caatcccgct cctgggcttg
    40861 cttcggtcat tttcgtgcac aataaagtca attgactggc aaatcaatca ggaggaacag
    40921 atgagcaagc aaaagtttga gcgcaaaaaa ccgcacctca atatcggaac cattgggcac
    40981 gtagaccacg gtaaaactac tttaactgcc gccattacta aggttctgtc caaacagggc
    41041 aaagcccaat ttctcgccta cgatcaaatc gacaaggcgc cggaagaaag agaaaggggc
    41101 gtgacgatca atattgccca cgtcgagtac gagactgata agcgtcatta tgcacacgtc
    41161 gactgccctg gtcacgccga ctacgtaaaa aatatgatca cgggggcggc gcaaatggat
    41221 ggagcaatcc tagtggtttc cgccgccgat gggccgatgc cgcagacccg cgagcatatc
    41281 ctgttggcgc gccaagtcgg tgtgcccggt atcgttgttt tccttaacaa ggctgacatg
    41341 gttgatgaca aagagcttct cgaattggtg gagctcgaag ttcgagagct gctctccaag
    41401 tacaaattcc ccggagacga agttccgata gtgatcggta gtgcgttgaa ggcattggag
    41461 ggagacgctt cggatatggg cgaggggtcg atcataaagc tcatggaagc cgttgattct
    41521 ttcattaaag agccaaccag agacgtcgat aagccattta ttatgccggt tgaggacgtg
    41581 ttcaccatca gcggtcgcgg cacagtcgta accggtcgag tcgagcgcgg cgtcattaag
    41641 gtcggggaag agatcgagat tgtcggcttt aagccgacgc aaaaaaccgt cgctaccggg
    41701 atcgagatgt ttcgcaagct tttggatgag ggtcgggccg gtgataatat cggggtactt
    41761 ctacgcggta ccaagcgtga agaggtcgag cgaggtcagg ttttggcgaa gcccgcgagc
    41821 ataaccccgc acactaaatt caatgctgag gcttatgttt taaccaaaga agaaggtgga
    41881 cgacacacgc cgtttttcaa cggataccgc ccgcagttct actttcgcac cacggacgtc
    41941 accggtgtcg tgaaacttcc cgaaggaact gagatggtta tgcccggcga caacatcaat
    42001 gtggagattg aattgattac tccggtggcg atggatgaag ggcttaagtt tgcaattcga
    42061 gagggcggtc ggaccgtcgg cgccggagtc gtcacaaaga tcatccagta acccaggcca
    42121 gtggaggcgc gctcatgcgg gacattgtag ggtttgcttg cgaccaatgt aagcgaaaga
    42181 attatacgtc gacgaagaat aagaaaaaaa ccaccgacaa gctctctttc aagaagtttt
    42241 gcgcttcatg tcggtctcat acgatacatc gagagatcaa agtttaacga tttaccgggg
    42301 ggtcagtagc tccaattggc tagagctccg gactccaaat ccggctgttg caggttcgaa
    42361 tcctgcctga cccgccatca acgcatcgtt agcctcatgg cagactggat tcaaaaactt
    42421 caggacacgc ttcgccatac gatcgagttc gtcaaggagt cctggcagga gctgcaaaag
    42481 gtccactggc cgtcgcggaa ggaaacctac gcggcgaccg cgctcgttat catagtggtc
    42541 gtgctggtgt cggtttactt ggcgataata gacctgggtt tgaccacaat gattcaggca
    42601 atcatagatt aatcctcaga gctctcctat ggcaaagcaa tggtacatcg ttcacacctt
    42661 ctcagggttt gagcaaaagg cgaaagcagc tctcgaagag cgggtaagga ctctcggtaa
    42721 acaggagtac ttcggtgaaa tccttgtgcc agttgaaaag gtcgttgagc tcgttaaggg
    42781 aaagaaaaaa acttcctcaa gaaaattctt ccccggttac attctcgttc aaatggaact
    42841 caacgacgat acctggcatg tggtgaagga gacgccgaag attactgggt tcgttggtgg
    42901 cagcacgaat ccgccctcaa tttcggaaga ggaggtaaga gccattactc ttcagatgga
    42961 agagggcgcg atcaagccca agccgagggt gctattttcg gtcggtgaga acgttaaagt
    43021 agtcgatggt ccgttcgcag atttcaacgg ggttgttgaa gaagttcgac ccgataaagg
    43081 caagcttcga gtgctcataa gcatctttgg acgagccact cctgtggagt tggaattcgt
    43141 gcaggtcgag cggaattagc taacaaggac gctataatgg ccaagaaagt tatcgcggaa
    43201 atcaagttgc agattccggc tggagcagct aatccaagcc ctcccgtcgg gcccgcattg
    43261 ggacagcgtg gggtcaatat catggaattc tgcaaggcct tcaatgctgc gactcaatcc
    43321 cagcaaggta tgatcacgcc ggtgattatc accgtctatg cggaccggtc tttcacgttc
    43381 gtgacgaaaa ccccgccggc ctctgtacta ctcaagaagg ctgcaggcgt cgagaaaggt
    43441 tccaaggaac ccgggaagac caaagtcggg aaagtcactc gggcgcaagt ccggaatatt
    43501 gcggagttga agctcaagga tctgacggct aaagatttgg ccgccgccga gaagtcggtc
    43561 gaaggtactg ctcgcagcct cggtctcgat gttgagggat gatgcatagc gcatcactca
    43621 atcgattgaa tcaaatcaac ttcgaacgtc catgaggggg acaaatgagt ggttttggaa
    43681 aatcctatcg cgctgcgttg gcgaaggtag atcgtagtca gcgctatcaa ttggatgaag
    43741 gtcttcagct tgtgcggcaa accgcccggg caaagtttga tgagaccgtt gagctagcga
    43801 tccgtttggg tgttgacccg cggcaagcgg atcaaaatgt acggggaaca gtgaccttac
    43861 cgcacggtat gggcaaggct gttagggtgc tcgctttcgc caaaggagaa aaggagaaag
    43921 aagcccagga agccggagcc gatttcgttg ggagcgatga actcatcaaa aagatcagcg
    43981 agggatggtt cgaattcgat aaggccgtag cgactccgga catgatggcc gcagtcggac
    44041 ggatcggtaa agtattgggc ccgcgcggtc tcatgcctaa tcccaagaca ggcacggttt
    44101 ctgccgatat tgcacgtgcg gttaaagaaa tcaaagctgg caaactggag tttagagtcg
    44161 ataaagcagg gatagttcat gttcccgtgg gcaaggcttc tttcgagcct gagaagctca
    44221 tagacaatgc cagagccgta ctctcagcaa ttctccgagc caaacctgct tcggccaaag
    44281 gcaactacgt tcgcggtgtg acgctatcta caacaatggg gccgggagtt aaaatcgatt
    44341 tatcgcagat ccgggctatg gctgcctaga tctgttcttt agctaaattt cagccaaaga
    44401 atggggagag aaggatggcg gagttagaag ctgcccgctc cagtcatggc gaaaaagccg
    44461 agacggtagc ggctgtccat gaaaaattca ggaaagcaaa aatggccatc gcgaccgaat
    44521 atcgcggact ttccgttagt aagatgaccc agttgcggcg cgagattcgt gacgcagcag
    44581 gagaatatca ggttatcaag aatactctcg tacggcgggc tcttaaggat acggcgtacg
    44641 gtgacttgga acgaatgctc gagggtccca atggatgggt gttcgcgtat gacgatccgg
    44701 tcttgctttc taaaacgctc gtcaagttcg ttgatgctaa tgaagcgatg acgatcaagg
    44761 cggcgatgtt tgagggcgag ttaatggatc ccgctcgggt caaggttctt tcacaaatgc
    44821 ccagcaagcc tgagctgcag gcgaccttgc tcgcgctgtt gcagacgcca gcgacttatc
    44881 tgctccgtct gattcaagaa cccggatctc gggtggtccg attactagat gcggcggcta
    44941 aggaaaaggc cggctaacaa gtaccatggt tgtttcgcaa tcaattaaaa gggggacgtt
    45001 ttatggcaga ggtatcgagg gaacaggtta aagactacat caagaatatg acgctgctgg
    45061 acgctgcaac tctagttaag gaactcgagg aggaattagg tgtcagcgct gcggcgccgg
    45121 ttgcggtagc tgcggcagct agtggcccgg ccgcagcagc ggaggagaaa acggagtttg
    45181 acgttgttct ttcagaagcg ggcgaaaaga aaattcaggt tatcaaggtt gtccgcgagc
    45241 tcaccggtct ggggttgaaa gaggccaagg atctcgtcga tggcgcgccc aagaatgtta
    45301 aagagggcat agccaaggcc gaggctgaaa caatcaagaa gaagctcgag gagcaggggg
    45361 ccaaggtcga gatcaaatag gggtaaacca agattcgggg aatcctgcgt aacaggagtg
    45421 ttgtttagat cgctaaagga gagacaatgc cgttccaagt ggccaacaat ttacgattcc
    45481 gccgcagttt cggacggatc aagagaatca ttgatctgcc ttacttgatt gaaatccaga
    45541 agaactccta tgagttgttt ttgcaaaagg atgcgtcagc cgcccagcgc gagaatatcg
    45601 gccttcagga agtttttaaa tcggtattcc caatcaagga tttcaacgag acagcgtccc
    45661 tcgagtttgt aagctatagc ttgggcgagc ctaaatacga tgtcgacgag tgtcaccagc
    45721 gcggcatgac tttcgccgcg ccgctgaagg ttaccgtcca gctcgttcta tgggatgtcg
    45781 atgcccagac cggcgctcgc agcatcaaga atgtcaaaga gcaggaggtc tactttggtg
    45841 aaatcccgct aatgacgaag aacggcacat tcatggtgaa tggcaccgaa cgagtcattg
    45901 tcagtcaatt gcaccgatcc cctggagttt ttttcgagca cgacaaggga aagacgcatg
    45961 ccagcgggaa attgctttat tcggctagaa tcatccccta ccgcggttcc tgggtcgatc
    46021 tcgagtttga tccacgagat gtcgtttatg tccgaatcga tcgccggcgg aagtttcacg
    46081 ctacggtcct gctgagggcg ctcggaatga ccacggaaga tctgctgaat tattattacc
    46141 ggagcgatac catcgttttc gacggccgca aaccggcgct cattttcaat cctgcgcatt
    46201 taatgggagc gaaagtgacg agcgatgttc gtgatccgaa aagcaacgac ctcatcgtta
    46261 aggaaggcaa gaagtttacc cggccggtct tgcgtcaaat ggagcaagcc aaggtcaaac
    46321 aggttccgat cgccttggaa gatattcttg gtcggatcgc atcccatgac atcgtcaatc
    46381 cccaaagcaa agaagtcatc gttgaatcca atcatcccat cacccaagaa aagttggacc
    46441 tcatccggga aaagggcatt catcaaatcg aagttctgtt cattgacgaa tcgacagttg
    46501 gtccgtacct gcgaaataca ttgttgcagg atcggattaa ctcacctcaa gatgcaattc
    46561 tagagatcta tcgccgctta aggccaggcg atccgccgac ggttgattcg gcgacgaact
    46621 tttttaacaa tttattcttc aatcccgacc gttatgatct ttccaaagtg ggtcgactca
    46681 agctgaatca tcggcttaag ctcaatgttc cattggagca aggtacgctt cgcaaggaag
    46741 acattcttga ggtcgttcgc tatttgatgg agctgaagaa cggtaatggc accgtggacg
    46801 atatcgatca tctcggcaat cgccgagtac gggccgtcgg cgagctggtt gaaaaccact
    46861 ttcgtgtcgg gctggtgcgt atggaacgag ccatcaaaga gaagatgagc ctgcaggata
    46921 tcgaaacttt gatgccgcag gaactgatta actacaagcc tgtttcggcg gcccttaaag
    46981 agtttttcgg ttcaagccaa ttgtcccaat tcatggatca gactaatccg ctttcggaga
    47041 tcacgcacaa acgccgatta tcggcattgg ggcccggtgg actgacccgg gaacgcgccg
    47101 gcttcgaggt gcgcgacgtt catccgacgc attacggtcg cgtctgcccc atcgagacgc
    47161 cggaaggtcc gaatatcggc ctgatcgcat ccttgacgac ttacgctcga gtcaatgagt
    47221 tcggtttcat cgaaacgccg tatcgcgagg ttgacaacgg caaggtcacg gatcggatcc
    47281 ggtatctttc cgcgcttgaa gaggaagacc atgtcatagc gcaggccaat gcgccgattg
    47341 atgcgcgggg cgtttttact ctggatctag tctccgctcg caaaggcggc gaattcgtaa
    47401 tggcgcgacc cgaggaagtc aacttcatgg acgtgtcgcc caatcaactc gtcagcgtgg
    47461 cggcctcgct gattccattt ctcgagaacg atgacgccaa ccgggctctg atgggctcta
    47521 acatgcagcg tcaagcagtg cctctgttaa ggaccgaggc tcctctggtc ggtaccggaa
    47581 tggagaaagt tgtcgcccgg gattccggcg tcacggttgt ggcgaagcgc gaaggcataa
    47641 tcgagagcgt cgattcgact cgcattgtgg ttaaggctga taaggcttcc ggcccccgcg
    47701 ataccggagt tgatatctac aatctggtca aataccagcg ttcgaatcag aatacttgcg
    47761 ttaatcaacg accgattgtc cggattggtg accaggtgaa agccggcgat gtcatcgccg
    47821 acggtccgtc cacggagatg ggagagcttg cgctcggccg caatgttctc gtcgcgctca
    47881 tgccgtgggg cggctacaac tttgaagatt ccatcttgat tagcgagcgc gtcgttaaag
    47941 aagacattta cacctcgatt catatcgaag aatttgaatg cgtgtcgcgc gacaccaaac
    48001 ttggtccgga agagattacg cgggatatcc ctaacgtcgg cgacgaagct ctgaccgatc
    48061 ttgacgacag cggcatcatc agaatcggcg ccgaagtgaa gcctagtgat attctcgtag
    48121 gaaaaatcac tcccaagggc gagactcagt tatctcctga agagaaacta ttgcgcgcca
    48181 tattcggaga aaaggctggc gaggtccgtg atacatcatt gcgcgttcca ccgggagttg
    48241 aaggcactgt catcaatgtc cgcatcttct cgcgcaaagg tatgggcaag gatgagagaa
    48301 gcaaggcgat cgaggacgaa gagatcatac ggctgcgcaa agatcagcat gatgaagtcc
    48361 gaatcctcca agacggcgcc ctccgacgtc tgaaaaaact tttagtcggc cgtaaactgg
    48421 caaaccggct cagcgacgat agtcgaaaac tgctgctcgt taagggtaag gagatcaccg
    48481 aagaagatct ggatcaattg ccgatgagct attggagcga gatccgcgtc gataacgaat
    48541 cagtggaagc ggagctcgac cggattgttg aaagcacggc cggacaggtc aagctgatta
    48601 aggaagtatt cgaagacaag atcgaaaagt tgcggggcgg cgatgaactc ccgccaggcg
    48661 tcatcaagat ggtgaaagtt ttcgtcgcta tcaagcgtaa gttgcaagtt ggcgacaaga
    48721 tggccgggcg ccatggcaat aagggtgtta tct
//
LOCUS       DASUVS010000351        12447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1897696, whole genome shotgun sequence.
ACCESSION   DASUVS010000351 DASUVS010000000
VERSION     DASUVS010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12447
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897696"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..793)
                     /locus_tag="VF182_18740"
     CDS             complement(95..793)
                     /locus_tag="VF182_18740"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6439165.1"
                     /translation="MIALEEVSFGVEPEEFVTVVGRSGCGKSTLLKITAGLLTATGGF
                     VRISGTTVQGPLTNIGVVFQAPVLLAWRKALDNVLLQIEARGLNVDEYRKRALELLDL
                     TGLRGFENKYPNELSGGMQQRVSISRALIHDPPLLLMDEPFGALDAITRDEMNLELLR
                     IWSESKKTVLFITHSIPEAVFLGDRVVVMTPRPGRVAEIMTIDLPRPRTTALRDDHKF
                     IGYVRKIRERLGVQ"
     gene            complement(863..1876)
                     /locus_tag="VF182_18745"
     CDS             complement(863..1876)
                     /locus_tag="VF182_18745"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439166.1"
                     /translation="MKTKMITLAAVLWLSGSIFFAGNSTAIAADKIKVQLDWILEGKH
                     VPFFVARDRGFFKKNNLEVALLEGKGSANSATFVDAGQADYSYGDLLTAIQVMSKGAK
                     NRAIGTGMAFNGGGLIFLESSGIKTAKDLEGKSFGTNPGDFAYILLPAAGAAAKFDHS
                     KVVIKTMEPAVRTPALFEGKIDFMSGTNGSSIQRMAILGKKQGKQIKYLFFKDLGLET
                     YGHVLQAREDRIKNNPDQVKRFVSAVFDAWAWSLKNPKEAFEIFMKANPQNERDISLA
                     QMEAGLDDIVDPETKDRGLGFMKEAMMKKSVDIANKYFELSPAVDYKITYTNQFIGKN
                     PGM"
     gene            complement(1918..2715)
                     /locus_tag="VF182_18750"
     CDS             complement(1918..2715)
                     /locus_tag="VF182_18750"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6439167.1"
                     /translation="MPVKMENPPSSGTPSIKWSALFFVCILVLWQLLLPLLKIPSYLL
                     PTPLEVLAEFQTRGGFIFAHFLPTFYETMLGFLIGALIGVVSALGIVYSRILWVTIYP
                     SLVVIGSVPRIAIAPLILIWLGIDSIWSKVTIAFLVAFFPMVVNSVVGFSQIEGDMLD
                     LAKTMGMKPWTEFRKIRLPNSIPYLFAGFKASIALAVIGAVVAEFVSGQQGLGYVIII
                     GNNNLNAALIFAAITLLSIMSLGLFGIIVGLEKVLMPWRRGIYPTEL"
     gene            3070..4008
                     /locus_tag="VF182_18755"
     CDS             3070..4008
                     /locus_tag="VF182_18755"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439168.1"
                     /translation="MERLDHLRLLQFRAGYNLPVHVAVEHGIFAKHDLEVEFAYTPGS
                     DYLIEALQTGKCEIAHPAADDLVAAVESEPHKRDSEIDLFLFMGLHSGLLSLIAAPEN
                     PDVESLRGKLLGVDSRRTGFVFLLEKFLRSKGLGPADYSLVEIGGWEFRYRALLNKQV
                     AATLLTSPFVADALSSGCHLLAHGDEILPVYQATCGAAKKAWARDHSEVLVRYIKAYI
                     AATRWCFNPVNRRASLDLLKKHSGLDDKRAENTLATLLEPNRGLYPKAKLNIPGLAAV
                     LALRAEMGHLKPPLPSPEKYIDLSYYRRAAEAAEAR"
     gene            complement(4037..5068)
                     /locus_tag="VF182_18760"
     CDS             complement(4037..5068)
                     /locus_tag="VF182_18760"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439169.1"
                     /translation="MKLIELARLKTASLLALLSVMGSLSFSPAYLRAAEKAKDIPITI
                     RLDFIVGGNHAPWFVASEKGFYAKRGLNVTIQPGTGSADTIRTIAAGGADVGFANVST
                     AIVGRSRGAAVVSVAQLGYIAVTVLWREDTDIKSLKDLERKSWAISPGQAQWFLLPAL
                     ARINNLDFKSIKIEETAPPLQPAALLAKKADFITMFRASNDEVAEMTAAKQGIRLKRI
                     HMRDAGLDIYGSALVVKEEDIKRRPEMIRAYVEGTMEGLRYTRDHQDEALRILLKLKP
                     ELDKELTRLQIKNGVEEVFIPAESLASGFGYMKPDVMEKTVKISNEFFDVAGKVAAAG
                     VYNNQFIGK"
     gene            complement(5106..5747)
                     /locus_tag="VF182_18765"
     CDS             complement(5106..5747)
                     /locus_tag="VF182_18765"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HEX6439170.1"
                     /translation="MANDSAMRRYPIPGTIVREWIIPAKEYSAFTMSRGQTLRFVDIE
                     GKQVPDLVCFNQFDLTEHLNLGNTLLLNKRRELRQGDVLYSVICNPMMTIVGYSNEES
                     YAYGPMCSEELNRIRYGVPGTRNCRDNFTMALAAQGLNRREIPNAFVPFMRVEVESNG
                     SMEIKEPTSRAGDFYDLRADTDLLVAVSNCPQERNPCNGFNPTPMGMIIYQPG"
     gene            6308..7828
                     /locus_tag="VF182_18770"
     CDS             6308..7828
                     /locus_tag="VF182_18770"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439171.1"
                     /translation="MRRHGTAIDRRQFLRQSAALGLATLVAPTFAARTWAATKDRIII
                     YQGVSLDSLHPYGYSGGGITGIWRHIIEPLIQMDYDRNEFVGVLAQTWEFQGRKWIFH
                     LRKGVRFHNGAPFTSKDVAFSIDKLQNDKRSLQGAYVQGIEVDTPDDYTLVLTTRTPN
                     ALLLTRLETRFILSKEYGDDLDSATAGTGPYKYVSWQRGGNLVLRRNDEYWGAKPPIR
                     EVIIKGVKEESARVAGLLAGQTDVISNVPIEDVDRIAKHPRVRLQEVVGFRMYFLGMN
                     VQMKPFDNKLVRQAINYALDPHVMLKHIYDGHGAVVNGPLGSNWVGFDPKIKRYPYDP
                     KKARELLAKAGYPNGLETKLYLAPDRHLKGKEVCEVIADQLGKVGIKAELVPQEYAIY
                     WGKDGVNGGKLPFYYAGSNGSDADVYFEQYFRTGGSKRINYNNPEFDKLIDEEQRTGD
                     HQKRVAILHQAGRILMEDVPFAPLYTLAELYGVQRNVIWKGSPDGKILAGDMKIRS"
     gene            7971..8735
                     /locus_tag="VF182_18775"
     CDS             7971..8735
                     /locus_tag="VF182_18775"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6439172.1"
                     /translation="MPFYDNGRVRIRYEEAGSGFPLLLVPGGGLNSTIDGLKRGVPFD
                     AIEEFKGEYRCIFSDMRNANGGDSTGPLELDRPWDAYTDDQIGVMDRLGIDKFVVMGF
                     CIGGPLIWNLLKREPDRIVAAVLAQPSGFRPELPNHSYDGNMKGWGPDLVKRRPEITM
                     EMVDKFLTKMYRTNADFVWTVTRDFVRNCRTPVLILPDDTKPHPYVVAMESAMLAPKA
                     EVSMFPWKEPKERIPLAVRQIRSFLRSHRLAPVSTV"
     gene            complement(9068..10258)
                     /locus_tag="VF182_18780"
     CDS             complement(9068..10258)
                     /locus_tag="VF182_18780"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HEX6439173.1"
                     /translation="MSAILATAESDAGKAPRASSNIRHLCRMEIEGGGQIVISGKFAY
                     VGHQHRPHGTTILDISDPGKPKILSTLTPGHPWSHSHKARIVGDLMIVNSEFERGDGA
                     RFHEYSDGGFRIYDVKDRTKPKLISFVKTHGKGVHRFDADENYAYISTEMKGYVGNLL
                     VIYDIRNPSKPVEVSRWWMQGQHVEGGDVPHPKHAEHRLHHALRSGNEMYAGCWMSGV
                     SIIDVNDISAPRTLSRYEYDPPCPEPTHTFLKVPHEIGGKRIAVSTEEERAHRGADAG
                     KPHAPLRTWDVTDPTKPKLLHSYYLPESASPYPAGKVRFGTHQLREKVDRDNLLYVTW
                     FAGGLRIIDINDPPNPKERGYFIPKPGDGREAPETNDVAKDDRGLLWVTDKQRGLDVI
                     EYQD"
     gene            complement(10688..11683)
                     /locus_tag="VF182_18785"
     CDS             complement(10688..11683)
                     /locus_tag="VF182_18785"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439174.1"
                     /translation="MARALKMLTLALFFTVFGFSQAAPAQDKKLEKIRIGGGSVGAPQ
                     MTMWFAKEANLYEKHGLAIEAISIPGSSMAIQAMLSGEVPIIQLGGTASMQANLAGAD
                     TVIVATVLKKFLFSIFSRPEITRIADLKGKLFGATRFGTLSDFASRFALEKYGLNPER
                     DITMVQTGGQPETVVALLTGKVQAAALSVPATMRAKKANMRELLDMATLEATIHQNGV
                     VTTRRYIKTNEDTVRRFLRAYIEGAALAKKDKAFAMRVMAKYLSTSDRELLDDAYERV
                     TSHLEIPPHPTVEGVGVLLKTLEKSQPKAREAKPEDFIDARPLREIEKSGFMDRL"
     gene            complement(12068..>12447)
                     /locus_tag="VF182_18790"
     CDS             complement(12068..>12447)
                     /locus_tag="VF182_18790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439175.1"
                     /translation="STSGCEREPAGADLAITQWISAAEPCQIRPSLLSFLQRTAYIQQ
                     LAAKKFRNFRSDITLRQHGFANELIAAIYYGSQSFSIALCEVIYFGRKSRVGRNALDP
                     SAAAAPPVPPASAEPYLILNVDR"
BASE COUNT         2718 a         3407 c         3500 g         2822 t
ORIGIN      
        1 ggcagaggat gtcgatctct attgccgaat ccgattcaaa acgatttgac ctacttgaac
       61 ggattgaact ctgcgggggc ggcgcttcta cggattattg cacgccgaga cgctcgcgga
      121 tttttctcac ataaccgatg aacttgtgat cgtcgcgcaa agcggtggtg cgcgggcgcg
      181 gcaagtcgat ggtcataatc tcagcgactc tgcccggacg gggcgtcatc accactacgc
      241 ggtccccgag aaacacggct tcgggaatac tgtgcgtgat aaaaagcacg gtcttcttgg
      301 actcgctcca gattctcagt agctccaaat tcatctcgtc acgagtgatg gcgtcgagcg
      361 cgccaaacgg ttcatccatc aaaagcaacg gcggatcgtg gatgagcgcg cggctaatgg
      421 agacccgttg ctgcatgccg cctgacagct cgttgggata tttgttctcg aaaccacgca
      481 agccggtaag atctagaagc tcaagcgctc tttttctgta ttcatcgacg ttcaagcccc
      541 gcgcttcgat ttgcagcagc acgttgtcga gcgccttgcg ccacgccagt agaacaggcg
      601 cttggaagac gacgccgatg ttggttagcg gaccttggac ggtggtcccg gagattcgaa
      661 caaatcctcc ggtggcggtt aaaagccctg cggtgatctt gagcagcgtg cttttaccac
      721 aaccgctgcg cccaactacc gtaacgaatt cctccggctc cacgccaaag gatacttctt
      781 caagcgcgat cacctcatcg ttgcctgaat tgtagatctt acggacccgg ttgtattcga
      841 tgaggctggt caatccggtt tcctacatgc ccgggttttt tccgatgaac tgattggtgt
      901 aggtgatttt atagtcaacc gctggtgaca actcaaagta cttgtttgcg atatcgactg
      961 attttttcat catcgcttct ttcataaaac caagcccgcg gtctttggtt tccgggtcga
     1021 cgatgtcatc gagtccggct tccatttggg ccaaactgat atcccgctcg ttttgcgggt
     1081 tggctttcat gaaaatttcg aaggcttcct tggggttctt aagcgaccat gcccatgcgt
     1141 caaaaaccgc ggagacgaat cgtttgacct gatccggatt gtttttaatg cgatcctcac
     1201 gtgcctgcag cacatggccg taggtctcta atcccaagtc cttaaaaaag aggtacttga
     1261 tttgttttcc ctgctttttt cccagaatcg ccattctttg gatgctcgaa ccgttggtcc
     1321 ccgacatgaa gtctattttg ccttcgaaca acgcgggcgt tcgcacagcc ggttccattg
     1381 tcttgatgac gactttgcta tgatcgaatt tggcggcggc gcctgcggcg ggcagcagga
     1441 tataagcaaa atcgcccggg ttagtaccga aacttttgcc ttcgagatct ttggcggtct
     1501 tgattcccga gctttccaag aagatcagcc cgccaccatt gaacgccata ccggtgccga
     1561 tggcgcgatt cttcgcgccc ttggacatga cttggatcgc tgtgaggaga tcgccgtaac
     1621 tgtagtcggc ctggccggca tcgacaaagg tcgccgaatt ggcagagcct ttgccctcga
     1681 gcagggctac ttcaagattg ttctttttaa aaaagcctcg gtcccgcgcc acaaaaaaag
     1741 gcacgtgctt tccttcgagg atccaatcca actgaacctt tattttgtcc gccgcgatcg
     1801 ccgtcgaatt gccggcgaaa aaaatgctcc cgctgagcca aagcaccgct gcgagggtta
     1861 tcatttttgt tttcatgatt gtcctccctg ttcagatttt gatcgagagc gcgcagctca
     1921 aagttccgtc gggtatatgc cgcgacgcca tggcatgagc actttttcca aaccgacgat
     1981 gattccgaat agtcccagcg acataatcga aaggagcgtg atggcggcga agatgagcgc
     2041 ggcattgagg ttgttgttgc cgatgatgat cacatagccc agaccttgtt ggccggagac
     2101 gaactcggcg acgacggcac cgatgacggc gagagcaatc gaggctttga agccggcaaa
     2161 tagatacgga atcgagttgg gcagacggat cttcctgaac tcagtccaag gtttcattcc
     2221 catggttttg gccagatcca acatgtcgcc ttcgatctgt gaaaatccca cgacggaatt
     2281 gaccaccatg gggaaaaaag ccacgagaaa agcaatcgta accttcgacc aaatggagtc
     2341 gatgccgagc caaatcaaaa taagcggtgc gatggcgatt cgcggtaccg acccaataac
     2401 gacaagcgat ggatagatcg tcacccagag tatcctcgag tatacgattc ccagggcgga
     2461 aacgactccg atcaatgcgc cgattaaaaa tcctagcatc gtctcgtaaa atgtcggtag
     2521 gaaatgggcg aagatgaaac cgccacgggt ttgaaattcg gcgagcactt ccaagggtgt
     2581 cggcagtaga tacgagggga tcttcagcag cggaagaagc aactgccaga gaactagaat
     2641 gcaaacgaaa aatagcgccg accatttgat ggatggcgtg ccgctagacg gaggattctc
     2701 cattttaact ggcatgatac ggacgttagc tggggttttg attatcgaaa gggaataatc
     2761 gtgtcaagga taaagaggga ttggcggcaa gatcgagcca ttcacagcag agtgcccgag
     2821 gagcgagcct ctcagggcgc tggcaatggg ctgtaatgga gtatggttca attcgttcaa
     2881 aacatctcga acggcttgaa cgtttcaacc cgctgtgccg gcaaggtcac cgctcgatca
     2941 atgcaatgcg gtagtttcct gaataggaat atgcgactag cttcagatcc gattgaccgt
     3001 tccagtgccg attacgttcg tgatagtttg ccgttgatgc gaaatacgat tcaagaaggg
     3061 aggcatccaa tggaacgatt ggaccatctg cgactgcttc agtttcgcgc cggctataac
     3121 ctgccggttc acgtggcggt agagcacggc atcttcgcca agcatgatct tgaagttgaa
     3181 ttcgcctata ctcccggttc cgattatctc atcgaggcgc tccagaccgg caagtgcgaa
     3241 atcgcccacc cggcagccga tgatcttgtg gcggcggttg aaagcgaacc tcataagcgc
     3301 gatagcgaaa tcgatctatt tctctttatg ggactccata gcggtctcct tagcctgatt
     3361 gccgcgccgg aaaatcccga cgtcgagtct cttcggggca aattacttgg cgtggattcc
     3421 agacgcacgg gtttcgtctt cttattggaa aagtttcttc gctctaaggg attaggaccg
     3481 gcggattaca gcctggttga gatcggtggt tgggagtttc gctaccgcgc tctgctgaat
     3541 aagcaagtcg cagcgacttt gctgacctcg cctttcgtgg ctgatgcttt gtcgtccggt
     3601 tgtcatctct tggctcacgg cgatgagatt ctacccgttt accaggcaac gtgcggcgct
     3661 gcgaagaagg cttgggcgag agatcatagc gaggtcttgg tccgatacat caaagcttat
     3721 atcgcagcca cccgctggtg cttcaatccg gtgaatcgcc gcgcctcttt ggacctgctc
     3781 aagaagcata gcggcctcga cgacaaacgc gcggaaaaca cgctggccac gctcttagaa
     3841 cctaaccgag gtctctatcc caaggcgaag ctcaatattc ccgggcttgc cgcggtgctg
     3901 gcgctgcggg ctgagatggg ccatctcaaa ccgcctctac cgtcaccgga gaaatacatc
     3961 gatctctcct attatcggcg cgcggcggag gctgcggaag cgaggtgatc gcgatgccgg
     4021 catgcccgaa cagtgcttac tttccgatga actgattgtt gtaaacaccg gcagctgcga
     4081 ccttgccggc cacgtcaaag aactcgttgc tgatcttgac agtcttttcc atcacatcgg
     4141 gcttcatgta gccaaatccc gacgctagcg attccgccgg aatgaatact tcctccacgc
     4201 cgttcttgat ttgcagtctt gtcaattcct tatccagctc tggtttgagc ttcaatagaa
     4261 ttcgcagcgc ctcgtcctgg tgatcgcgag tgtatcttag accctccatg gttccctcga
     4321 cgtaggcgcg aatcatttcg ggtcgacgtt tgatatcctc ttccttgact actagagcgc
     4381 tgccgtagat atcgaggccg gcatcgcgca tgtggattcg ttttaatcga atgccttgtt
     4441 tagccgccgt catctcggcc acttcatcgt tggaggcgcg gaacatcgtg atgaagtcgg
     4501 ccttcttggc cagcaaagcc gccggctgga gaggcggagc ggtctcctcg attttgatcg
     4561 acttaaagtc taagttgttg atcctggcaa gcgccgggag cagaaaccac tgcgcctgtc
     4621 cagggctgat ggcccaggat tttctctcga gatccttgag agacttgata tcggtatcct
     4681 cgcgccagag aaccgtgacg gctatgtagc cgagctgggc gaccgaaacg accgcggcgc
     4741 ctcgggaacg gccgactatc gcagttgaaa cgttggcgaa acccacgtca gcgccgccgg
     4801 cagcgatggt gcggatcgta tccgctgaac ctgtgccggg ctggattgtg acgttcagtc
     4861 cgcgcttggc gtagaagcct ttctccgagg caacgaacca cggcgcatga tttcctccga
     4921 caatgaaatc gagccggatg gtaatcggaa tatctttggc tttctcggcg gctctcagat
     4981 aggcggggga gaaactcagg ctgcccatga cactaagcaa agccagtaaa gaggctgtct
     5041 ttagccgcgc cagctcaatg agtttcatca ttctctccca acagtgggta tgcgtcactt
     5101 tgcctttaac caggttgata gatgatcatg cccatcggcg tgggattgaa accgttacag
     5161 gggttgcgtt cctgcgggca gttagacacc gccaccagca ggtccgtgtc cgccctgaga
     5221 tcgtagaagt cacccgcgcg gctggtcggc tctttgattt ccatggagcc gttggactca
     5281 acctccaccc gcatgaacgg gacaaaggcg ttggggattt ctcgccggtt caagccctgg
     5341 gccgccagcg ccatggtgaa gttgtcacgg caattgcgcg tcccgggcac gccgtagcgg
     5401 atgcggttca gctcctcgct gcacatggga ccgtaggcgt agctttcttc gttggagtag
     5461 ccaacgatag tcatcatcgg attacaaatg acggagtaaa gcacgtctcc ctggcgcagc
     5521 tcgcgccgtt tgttcagtag cagagtgttg cctaggttca ggtgttccgt cagatcaaac
     5581 tggttgaagc aaaccaaatc gggcacctgt ttgccttcga tatccacgaa ccgcagagtc
     5641 tggccgcggc tcatcgtaaa agcagagtac tctttagcag ggataatcca ttctctaacg
     5701 atcgtgcccg gtatgggata gcgtcgcatc gctgaatcgt tcgccattgt tcgacctctt
     5761 gttttttgtc aggcgtagtg aacaccgaca gcggatctgg aactacccag tcattactcc
     5821 aaggcaaatt taatgtccag tttcgctaag ttgaagagtc aagtgtttta tagatctgcc
     5881 cgcggattcc accgacagcg atgcaggtgc tcgtcgattt cgataccggt ggtaacaacg
     5941 gtcttctgcg cggggaccgg cgatgatcgc atgctctggc gataacgccc gggccattgt
     6001 ctttggtgaa aaatgaatgc cttggaagtc atcattcaat gagcaccgcg agcaaggagc
     6061 tgcgccgact tatttgttat ctgttgatga tcggatctta aacaagcagt tggtgcggtc
     6121 cgacctacca gggtttcttc ctaccattac aaacgctgtg acgccacgcg gcaacacagt
     6181 ttctcgtgta taggggcgag tctccgctgt ctcacgccgt accattgcgc ttaatgcctg
     6241 gtaaatttcg ttgacaggtc cgtcgtgggc tgagtatatg aggttcaatt tagcggggag
     6301 aactccaatg cgtagacatg gcacagcgat tgatcgacgc caattcttgc ggcagagcgc
     6361 agctctagga ttggcaaccc tcgtcgcccc tacgttcgct gcaaggacgt gggcggcaac
     6421 caaagatcgc atcatcatct atcaaggcgt cagtctggac tcgctccatc cttacggcta
     6481 cagcggcggc ggcatcacgg gcatctggcg ccatatcatc gagccgttga ttcaaatgga
     6541 ttatgaccgc aatgaatttg tcggcgtgct ggctcaaacg tgggagtttc aggggcgcaa
     6601 gtggattttc catctgcgca aaggcgtgcg ttttcacaac ggcgcgccgt ttacctcgaa
     6661 ggatgtagcg ttctccatcg acaaactgca aaacgacaaa cgcagcctgc agggcgccta
     6721 cgtgcaaggc atcgaggtcg acacacccga cgattacacg cttgtgctta cgaccaggac
     6781 tcccaatgcg cttttgctca ccaggctgga gacgcggttt attctctcta aggaatacgg
     6841 cgacgatctg gacagcgcca cggccggtac cggaccgtac aaatacgtaa gctggcagcg
     6901 cggcggcaat ttggtgctgc ggcgcaacga cgagtactgg ggcgccaagc cgccgatccg
     6961 cgaggtgatt atcaaaggcg tcaaagaaga atccgcgcga gtggcgggac tgttggcggg
     7021 gcagacggat gtgatcagca atgtgcccat cgaagacgtc gaccgcatcg ccaagcaccc
     7081 gcgcgttcgg cttcaggagg tggtcggctt tcgcatgtat tttctcggca tgaacgtgca
     7141 gatgaaacct ttcgacaaca agctcgtgcg ccaagcgatc aactatgcgc tcgatcccca
     7201 cgtcatgttg aaacacatct acgacggcca tggcgcggtg gtgaacgggc cgctgggctc
     7261 gaattgggtc ggcttcgatc cgaagatcaa gcgctatcct tacgacccga aaaaagcgcg
     7321 cgagctgctg gccaaagccg gttatccgaa tggactcgaa accaaactgt atttggctcc
     7381 ggatcgccac ctcaaaggca aagaagtttg cgaagtgatc gctgatcagt tgggcaaggt
     7441 cggcatcaaa gccgagctgg tgccgcagga gtacgccatc tactggggca aggacggcgt
     7501 caacggcggc aagctgccgt tctactacgc cggcagcaat ggcagcgacg ccgacgtcta
     7561 tttcgagcaa tacttccgca ccggcggttc caaacgcatc aactacaaca atccggaatt
     7621 cgacaagctc atcgacgagg aacaacgcac cggcgatcac cagaagcgcg tcgcgattct
     7681 ccaccaggcg ggccgcattc tgatggaaga cgtgccgttc gcgccgcttt acacgctcgc
     7741 ggaactttac ggtgtgcagc gaaacgtgat ctggaagggc tcgccggacg ggaagattct
     7801 tgccggggac atgaaaatca ggagctgaac gactacgagg atggatagca ggccgcagaa
     7861 cctgacgact ctaagcgctg cgcaaaattc tcaaagccga tgtcgccgca acgttgacgg
     7921 ccgcagcgcg gcgccttttt ggtcagcaga ttggacaagg ggaaatcgtc atgccgtttt
     7981 acgacaatgg ccgcgttcgc attcgctatg aggaggccgg ttccggcttt ccattactac
     8041 tcgtccccgg cggtggattg aactcgacga tcgacggctt gaagcgcggc gtgccgtttg
     8101 acgccatcga agagttcaag ggcgagtatc gctgtatttt ctccgacatg cgcaacgcca
     8161 atggcggtga ttccaccggc ccgctcgagc tcgaccggcc atgggatgcc tacaccgatg
     8221 accaaatcgg cgtaatggat cgtttaggca tcgacaagtt cgtggttatg ggcttctgca
     8281 tcggcggccc cttgatttgg aaccttctga agcgggagcc ggatcggatc gtcgccgcgg
     8341 tactggcgca gccaagcgga ttccggccgg agctgcccaa ccactcctac gacggcaaca
     8401 tgaagggctg gggccccgat ttggtcaagc gccggcccga gatcacgatg gagatggtcg
     8461 acaaattcct gactaagatg taccgcacca acgccgattt cgtctggacc gtcacgcgcg
     8521 acttcgtgcg caactgccgc actcccgtat tgatcttacc ggacgatacg aagccgcacc
     8581 cgtacgtggt cgccatggaa agcgcgatgc tcgcgccgaa ggctgaagtg agcatgttcc
     8641 cttggaaaga gccgaaggaa cggattccgc tggcggtacg ccagatccgt tctttcctcc
     8701 ggtcgcatcg gctcgctcct gtttcgacgg tgtagccgct caggagcgat tgacagcgtt
     8761 ctcacgtgat gttcggaaaa cacgttgatt gaatttacat accagacgaa aaaccccatg
     8821 gcttacacgc tcgatgagtt cgccacacga cggtggtgga gaaacgcccc aactttcgcg
     8881 gctccgcttg agcgttaaag attgcgcgcg aatccaatcc ttctgtgagg atagataccg
     8941 gcaaatcacg acggcggagc aacatccaag acctgaccta agctttacgt ttgtcgcatc
     9001 ccgcgtagcg agatctcctt aaatcaatct tcacgcccga ctccatgaac ctgcgcagcc
     9061 gccctgttca gtcttgatac tcgatgacat ccaagccgcg ctgtttgtcg gtcacccaca
     9121 acagcccgcg gtcgtccttg gcgacatcgt tggtttcggg cgcttcgcga ccatctcccg
     9181 gtttcggaat gaaataaccg cgctccttag gattgggagg gtcgttgatg tcgatgatgc
     9241 ggagcccacc cgcgaaccag gtgacgtaga gaagattgtc ccggtccacc ttctcgcgca
     9301 gctgatgggt gccgaagcgg accttgcccg cagggtaggg cgacgcagac tcgggcaagt
     9361 aatacgagtg aagcagcttg ggcttggtcg gatcggtgac gtcccaggtg cgcagcggcg
     9421 cgtgcggctt gcccgcgtcg gcgccgcgat gagcgcgttc ctcctccgtc gagaccgcga
     9481 tccgcttgcc gccgatctcg tgcggcactt ttaaaaaggt gtgggtcggc tctgggcagg
     9541 gcggatcgta ctcatagcgc gatagcgtgc gcggcgcaga gatatcgttc acatcgatga
     9601 tcgatacgcc cgacatccag caacccgcgt acatctcgtt gccgctgcgc agggcgtgat
     9661 gcaggcggtg ctcggcgtgt ttgggatgcg gcacgtctcc gccctcgaca tgctggccct
     9721 gcatccacca gcgcgacact tcgacgggtt tggacggatt gcggatatcg taaatgacca
     9781 ggaggttgcc gacgtagccc ttcatctcgg tggagatata ggcgtaattc tcgtcggcgt
     9841 cgaaacgatg cacgcccttg ccgtgggttt tcacgaagct tatgagcttg ggcttggtcc
     9901 tgtccttgac gtcgtagatg cgaaaacccc cgtcggagta ttcgtgaaaa cgcgcgccat
     9961 cgccgcgctc gaattctgaa ttgacgatca tcaggtcgcc gacgatccgc gccttgtgcg
    10021 agtgcgacca gggatggccg ggtgtaagcg tgctcaagat cttcggcttg cccgggtcgg
    10081 aaatgtcgag gatcgtggtc ccgtgcggcc ggtgttgatg gccgacgtaa gcaaactttc
    10141 cgctgatgac gatctgtcct ccaccctcga tctccatccg gcagagatgg cgaatattcg
    10201 aagacgcgcg cggcgccttg ccggcatcgc tctcggcagt cgctaaaatt gcgctcatcg
    10261 ggcacctccc atcaattgct ctgacgtctg cgaccggcga cgattcatcg tcgtaaaata
    10321 gtcgggattg gcgcttatgg caaaggccgt cggcgctacg ccgtattgat cttgccggac
    10381 gaaacgaaac cgcacccgta cgtggtcgcc atggaaagcg ggatgttcgc gccgaaggct
    10441 gaagtgagcg tgttcccttg ggaagagccg aaggggcgga ttccgctggc ggtgcgccag
    10501 atccgttctt tcctccggtc gcatcggctc gctccgggct aagtgaagaa gctcggctac
    10561 ttctgtcaga aggacggaac aataccattg cgaggaaagg cgtgggggat ccacggaact
    10621 gtgccactgc aagacttgac caataactga agacccctag ctgagttgac cctgattttc
    10681 tccagtccta gagccggtcc atgaacccgg atttctcaat ctctcggagc gggcgggcat
    10741 cgatgaaatc ttccggcttg gcctcgcgag ctttcggttg gcttttttcc aaagtcttaa
    10801 gtaaaacgcc aactccttcc acggtgggat ggggcgggat ttcaagatgg ctcgtgaccc
    10861 gttcataagc atcgtcgagc aactcgcggt cgctggtcga gagatatttc gccatcacgc
    10921 gcatcgcgaa cgccttatcc tttttagcga gtgcggcgcc ctcgatgtat gctctcaaaa
    10981 accggcgcac ggtgtcttcg ttggtcttga tatacctgcg agttgtgacc accccgttct
    11041 gatggatcgt cgcttccaag gtcgccatgt cgagtaactc gcgcatgttt gcctttttcg
    11101 cgcgcatggt tgccggcacg gaaagcgccg ccgcctgaac ctttcctgta agcagcgcga
    11161 cgacggtttc gggctgcccg ccggtttgga ccattgtgat atctcgttcg ggattgaggc
    11221 cgtatttttc aagagcaaag cgcgacgcga aatcggacag agtgccaaaa cgagtagcgc
    11281 cgaacagctt ccctttgaga tcggcaatgc gagtgatttc gggacggctg aaaattgaga
    11341 agagaaattt cttaagcact gtggcgacaa tcacggtgtc ggcgccggct aaattggcct
    11401 gcatcgacgc tgtgccgcct agttggatta tcggaacttc gccggagagc atcgcctgaa
    11461 tcgccatgga gcttccgggg atggagatcg cttcgatggc aagtccgtgc ttctcgtaaa
    11521 ggtttgcttc tttggcgaac cacatagtca tttggggcgc tcccaccgag ccgccgccga
    11581 ttcggatctt ttcgagcttc ttatcctggg caggagcagc ctgggaaaat ccgaatacag
    11641 tgaagaacaa agcaagcgta agcattttca gcgctctcgc catgaacacc tccgaatgag
    11701 tttcgatggg ctttcagtaa caaagcagcg gggcgtattt caagcgatca ttgaactgga
    11761 agttgaaaaa gagctagcgt gatactccct tcttcgaccg ccaggttcga acgattcacg
    11821 gttgctgtaa gtgcgtcaac taaacgcttg tccgttttga aactccaatc cgtcgacccg
    11881 acccacagaa cgcttcgtaa agagcgctcg cttccacctc ggcaagccaa cggctttctc
    11941 cgataaagcg aggattattc gaagcgcaat tgaaagagaa tcagttggac acgtgtaaac
    12001 gcgtcaagca agatatcgtt cgcaatcatg aagtgtcctc cctagcctgc gcttactact
    12061 gcgctgttca acggtcaaca ttcaaaatca ggtagggctc tgctgatgca ggcgggacag
    12121 gtggcgcggc tgcagcgctc ggatcaagag cgttgcggcc aactcgggat tttcgcccaa
    12181 aatatattac ctcgcaaagg gcgattgaga acgattgaga accgtagtaa atggccgcga
    12241 tcaattcatt cgcgaagccg tgttgtcgca gggttatatc gcttcggaag ttgcgaaatt
    12301 tctttgctgc cagctgttga atgtaagccg tgcgctgcaa aaaacttaat agactcgggc
    12361 gaatctgaca cggctcggct gctgatatcc attgcgttat tgcaagatct gccccggcag
    12421 gctctcgctc gcagccactg gtgcttg
//
LOCUS       DASUVS010000352         4537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1243584, whole genome shotgun sequence.
ACCESSION   DASUVS010000352 DASUVS010000000
VERSION     DASUVS010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243584"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VF182_18795"
     CDS             <1..226
                     /locus_tag="VF182_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439176.1"
                     /translation="VHHSFTVAPETLEQACRIFSEKNVAIDRLYYRAKGYFPGRELYF
                     FDPSGNRLELRDPTWKAGMPEPTFEELARS"
     gene            complement(502..1338)
                     /locus_tag="VF182_18800"
     CDS             complement(502..1338)
                     /locus_tag="VF182_18800"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6439177.1"
                     /translation="MSEPQSLIVYEKRDPHIAVIRLNRPEKKNSISRDLYWALDGAWH
                     KAKDDDDVWSIILTGTADAFCVGGDLKENLAFAKGEMTGPRSGPRQYSNLRALQMHKP
                     IIAAINGFAVGGGFNLALACHIRYCVPTAKFGCSEVRWSHMAAWPSYVCQLPTGWAYW
                     FALTGQMVDADTAFRLGLVQKICDPDKLIDDCVELCTTINRNGRLIAAHTTEYIEDRL
                     LYEIQGYEKGFEVHREYYAHLRQSKDYDEGSAAFTERRKPEFSQEYYDNKTKLPGVPE
                     TK"
     gene            complement(1408..2616)
                     /locus_tag="VF182_18805"
     CDS             complement(1408..2616)
                     /locus_tag="VF182_18805"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6439178.1"
                     /translation="MAKLPLDGIRVIELTTGAAGPTVARVLCEFGAEVIRCETRLRGD
                     GHRGEDPKLWNKKPDFMKLQRGKKSFTVNMQTPKGRELVKQLLKKSDVLVENFGLGIL
                     EKWGLDWPQLKDINPRCILIRVKGMGCTGPHAADLTYGPNVGNTMGTTYLWNYPGAAT
                     ATAEPRTQHPDFMGGVTGAFAVVLALIHRKRTGRGQWIDSAQVEIGASLLGPKYLEYS
                     VNHREPQPQGNRSLVAAPYGPYQCSGDDRWCVISVYNDQEWERVASLLEKAGLRRDAR
                     FATHLQRVRHHQELDNWITSWTKQHDPYELMEELQRIGVSAAVVQDVEDQFKRDQQYA
                     ATGFLVNMTEPEAGDVVTEGVPIRLSSTPGKVRGLAPLMGEHTHEIARELLGLTDDEI
                     RKLDDEKVLY"
     gene            complement(2791..4002)
                     /locus_tag="VF182_18810"
     CDS             complement(2791..4002)
                     /locus_tag="VF182_18810"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6439179.1"
                     /translation="MSESNQQAPLAGCRVLDIGGPLALHCTKLLADMGADVIKIEPPS
                     GDQSRRMPPFKGDSPHSEKSLYFLHFNTNKRGITLDIEKADGRAILLELTGKADVVIE
                     TFRPSRAKELRLTYQDLSASSPGLIVASITPFGQSGPWRDYKADDIAGLALGNLLYLA
                     GEPGEPPLQPPGEIAYGMASTYGAFGIATALYHRLESGRGQHIDVSMHECSGHIAGYF
                     IPNYGYTGAKPARASRKGEETDLYDPYATKNGYARIFIIPVEQWRRLVDWMGRPPAIS
                     GPEFEKMSYRRQHPDVVVGAIAEFCARHTKEELYEEGQKRRIAVTPINTAGEFINSEQ
                     VKERQIFVDMEHPVIGKYAHFGPVPRLSESPGRVARTAPLLGQHNQEIYCGELGMSND
                     DLVALRASGVI"
     gene            4168..>4537
                     /locus_tag="VF182_18815"
     CDS             4168..>4537
                     /locus_tag="VF182_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439180.1"
                     /translation="MSKLLVTLLIGCAAMIAPRFSLAAGAIDQVTVGYSSFSGAYVPL
                     WVAVEEQLGRKYGLELKAIYAGRVRPQQLLASGEVPFVIATGTGALSSHILGVKDQVI
                     ILTFINKVNSGLFTRADIKSA"
BASE COUNT          903 a         1279 c         1287 g         1068 t
ORIGIN      
        1 cgtccaccat tcttttacgg tggcccctga aactctggag caggcgtgca ggatattttc
       61 cgaaaagaat gttgccatcg atcgcctgta ctatcgtgca aaaggctatt ttcctggaag
      121 ggaactctac ttcttcgatc ccagcggcaa tcgtctcgaa ctgcgcgacc cgacttggaa
      181 ggccgggatg ccggaaccta ctttcgagga actcgcgcgc tcctgatcgc gaattgttta
      241 aacggctgaa acagttcgag tcgttcaaaa cgtctagcgc agatctcttg agagttcagt
      301 tggcctgaaa actagggacg caggctgttg caaccagccc ccgaactgac ggtcagaaac
      361 aagctggtaa attcaacact gctcggcagc tagatcgaaa gcagcgaaag ttcggacagt
      421 tgaaagattg caacggcttg aacgattgag acatatgcaa cgacccgatc gctcaagccg
      481 gcgacgcaga attgatgatt gttatttggt ttccggcact cccggcagtt tcgttttgtt
      541 atcgtagtat tcctggctga actcaggctt gcgtctctcc gtgaaggcgg cagaaccctc
      601 atcatagtct ttgctctggc gcagatgcgc ataatattct cggtgcactt cgaatccttt
      661 ttcgtagccc tgaatctcat agagcagtct gtcttcgata tattcggtcg tatgggcggc
      721 gataaggcgg ccgttgcggt tgatggtcgt gcataactcg acgcagtcat caatgagctt
      781 gtcgggatcg caaatcttct gcaccagacc taatcggaac gccgtgtccg cgtcgaccat
      841 ctggccggtg agcgcaaacc agtaggccca gcccgtcggc agttggcaca cgtagctcgg
      901 ccacgcggcc atgtgggacc agcgcacttc ggagcaaccg aacttcgccg tggggacaca
      961 ataacgaatg tgacatgcca gggccagatt gaagccgccg ccgaccgcga acccattgat
     1021 cgcggcgatg atcggtttgt gcatctgcag cgcgcgcaaa ttgctgtact gacgcgggcc
     1081 tgatcttggc ccggtcatct cgcctttggc aaacgcgaga ttttccttca agtcgccgcc
     1141 gacgcaaaag gcgtcagccg tgccggtaag tatgatcgac caaacgtcgt cgtcgtcttt
     1201 ggccttatgc catgctccgt ccagcgccca gtagagatcc cggctgattg aatttttctt
     1261 ttccggccga ttgagacgaa taactgcgat atgtgggtca cgcttctcat agacgataag
     1321 cgattgtggt tctgacatga gtcctccgtt gtgattagat ccgataaata agctgctctt
     1381 ctccggtgtg ctgtcggtcg tctccgtcta gtacaacacc ttttcgtcgt cgagttttct
     1441 gatctcgtcg tccgtgagac cgaggagttc gcgggcaatt tcgtgcgtgt gctcgcccat
     1501 caacggcgcc aggccgcgta ccttgcccgg cgtcgaagaa agccggatcg gcactccctc
     1561 ggtgaccacg tcgccggctt cgggctctgt catattcacc agaaatccgg ttgcggcgta
     1621 ttgttggtct cgtttgaatt gatcttcaac atcctgaact acggccgcgc tcacgccgat
     1681 gcgctgcagt tcttccatga gctcgtacgg atcatgctgc ttggtccaac tggtaatcca
     1741 gttatccaat tcttgatggt ggcgaacgcg ctgaagatgg gtagcaaatc tggcatcgcg
     1801 ccgaagcccg gctttttcca gcaagctcgc aacgcgctcc cactcctggt cgttgtaaac
     1861 tgagatcacg caccaacgat cgtcaccact acattggtag gggccatagg gagcggccac
     1921 caagctgcgg ttaccctgcg gctgcggctc gcgatggttg accgagtatt ccagatattt
     1981 gggccctaag agcgaggcgc cgatctccac ctgagcgctg tcgatccact gaccgcggcc
     2041 cgtgcgtttg cgatgaatca gcgccaaaac tacggcgaat gcgccggtga ctccacccat
     2101 gaagtcgggg tgctgagtgc gaggctcagc ggtggctgtt gcggcacccg ggtaattcca
     2161 cagataggtg gtgcccatgg tgttgccgac gttgggtccg taggtgagat cggccgcgtg
     2221 cgggccggtg cagcccatgc ctttgacgcg aatcagaatg cagcgcggat tgatgtcttt
     2281 gagttgcggc caatccaagc cccatttttc cagtatgccc aagccgaaat tttccaccaa
     2341 gacatcggat tttttgagga gctgcttgac gagttcgcga cccttcggcg tctgcatgtt
     2401 aacggtaaac gactttttac cgcgctggag cttcatgaaa tccggtttct tgttccacag
     2461 ctttgggtcc tcaccgcggt ggccatcacc gcgcaagcgc gtttcacagc gaatcacttc
     2521 cgcgccgaat tcgcacaaca cccgggcgac ggtcggtccc gccgcgcctg tggtcagctc
     2581 gatgactcgg attccgtcaa gaggtagctt ggccatatgt tctttcttca aagacgattt
     2641 ttactgcgga ggcgcggagt acgcagctta aaggaatctt tgccaaacat gctccgaacc
     2701 ccgcgaactc tgtgcctcgg tggtgaaatg cacctgcccg tttactggcg tgctgccgct
     2761 ctatgtgctc gatgatgaga ttctgacgca tcaaatcacc ccgctggcgc gcagcgccac
     2821 gagatcgtca ttgctcatgc ccagctcgcc gcagtagatt tcttgattgt gttgtccgag
     2881 cagcggcgcg gtccgcgcta ctctgcccgg cgactcgctc agtctcggca ccggaccgaa
     2941 gtgcgcgtac ttcccgataa ccgggtgctc catgtctacg aagatttgcc gctctttgac
     3001 ttgttcgctg ttgatgaact ccccggccgt attgatcggg gtaaccgcga tacggcgctt
     3061 ctgaccttct tcatagagtt cctctttggt gtgccgggcg cagaattccg cgatggcacc
     3121 gaccactacg tcggggtgtt gcctccgata ggacatcttt tcgaactccg gtccggagat
     3181 cgcaggtggc cgccccatcc aatctaccag ccggcgccac tgttcgaccg gaatgatgaa
     3241 gatgcgcgcg tatccgtttt tcgtagcata cggatcgtag aggtcggtct cttcgccttt
     3301 gcgcgaagcg cgcgcgggct tcgccccggt gtaaccgtag ttggggatga aatagccggc
     3361 gatatggccg gaacattcgt gcatcgacac atcgatgtgc tggccgcggc cgctctctaa
     3421 tcgatgataa agcgcggtag cgatgccgaa cgcgccatag gtcgacgcca tgccgtaagc
     3481 gatttcgccg ggcggttgta gcggcggctc cccgggttcg cccgccagat acaacaaatt
     3541 gcccagagct aatccggcta tgtcgtccgc cttgtagtcg cgccaagggc ccgactgacc
     3601 gaagggagta atcgaggcga cgatcaatcc gggactgctt gcgcttaggt cctggtatgt
     3661 gaggcgcagc tctttggcac gcgaagggcg aaaggtttca atgacgacat cggctttacc
     3721 ggttaactcg agcaggatcg cgcggccatc agctttctcg atgtcgagtg tgatgccacg
     3781 tttgttggta ttgaagtgca aaaaataaag actcttctcc gaatgcggtg aatcgccttt
     3841 gaacggtggc attcgccgtg actggtcacc cgagggtggc tcgatcttga tcacgtcggc
     3901 gcccatgtcg gccaagagtt tcgtgcaatg gagcgccaag ggtccgccga tatcaagaac
     3961 tcgacagcct gccagcggcg cctgctgatt ggattcactc atagatttcc tgttgaatag
     4021 cgatgtgcga tagtcggtac agttactgcg gatcaggcaa cgctgtcaag caggagagcg
     4081 gtttttgctt gatcgatgtt tctttcaggt gttaccgagc gaatatcgag accggctgca
     4141 accgttcaca aggaggatct cgtgacgatg agcaaactat tggtgactct tcttatcggc
     4201 tgcgcagcga tgatcgctcc tcgcttcagc ttggccgccg gtgctatcga tcaggtgacg
     4261 gtcggttact cttcgttctc cggcgcctac gtgccgctct gggttgccgt cgaagagcaa
     4321 ttggggagaa aatatgggct ggagttgaaa gcgatctacg ccggcagagt ccggccgcag
     4381 caactgctgg ccagcggcga ggtcccgttt gtcatcgcca cgggcacggg cgctctaagc
     4441 tcgcatattc tcggcgtcaa ggatcaagtc atcatcttga cgttcatcaa taaagttaac
     4501 tcggggcttt tcacccgggc tgatatcaag agcgccg
//
LOCUS       DASUVS010000353        14077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_806451, whole genome shotgun sequence.
ACCESSION   DASUVS010000353 DASUVS010000000
VERSION     DASUVS010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14077
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806451"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..1314
                     /locus_tag="VF182_18820"
     CDS             301..1314
                     /locus_tag="VF182_18820"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX6439181.1"
                     /translation="MASSLAPLPLRRGAWKPPRKIARWDAPGVLQKTGNLGEGFMIPM
                     MNIYDTIRTSLHFNKFVVGELLFVEYKCPIEQEVAGVWTPMDCFVHVLSGKKTWRTTD
                     GTLTAEKGQTLFFKKGAAVVYQDFKDDFCVMDFFVSDNFIRDVVREVSGQLRGRPAGD
                     AFQKAAILVNGDVTLSAYFQSMLAYFTGTAKPAETLLTLKLKELIVSILLGGKNPELA
                     GYFQSLLKSDAPVLSQIMEANFSYNLALEDFARLSHRSLSTFKRDFRKYYNQSPGKWL
                     LQKRLDYSAVLLKNPALNVSQVALDCGFEDLSHFSRAFKGKFGTSPANYRKGNSRSRL
                     LAG"
     gene            1729..2832
                     /locus_tag="VF182_18825"
     CDS             1729..2832
                     /locus_tag="VF182_18825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6439182.1"
                     /translation="MNYSNASHWTEVAHSLGGEFATRAAAHDEQDTFVSANYAALKER
                     GVFSAAVPQELGGGGASHREISDMLRTLAQYCGSTALALSMHQHLIAATVWKYRQGQG
                     GEPLLKKVAENQLVLVSTGAGDWLESNGSMSKADGGYVVSARKHFASQSAVGDMLVTS
                     AVFMDEVLHFPVPMNAPGVSVLNDWRALGMRGTGSHTVKLENVFVPEAAIAVRRPRGA
                     YHPVWNVVLTLAMPLIMSVYVGIAQKAVELSVSHIRRKKNRKPQVPSLIGEMHNDLVA
                     AEIQLNDMLRIANNYEFEAIDQNGQDILARKVNVANATIGVVTKAMEIVGGEGLYRDF
                     GLERLFRDVQGARYHPLPEAEQVKFSGEYLLRD"
     gene            complement(3256..3678)
                     /locus_tag="VF182_18830"
     CDS             complement(3256..3678)
                     /locus_tag="VF182_18830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEX6439183.1"
                     /translation="MEWKEALPLLQENHTGVATSITTEGRAQSTVVSTAVLDGKVGFA
                     SRPRTIKLKNIQRTGRATLTVIKLDNRRYVTVEGPASIEPWEDSQHHITRLRDLYISM
                     GRAPKGTDEEFARQMRDEERSIILVTPERLYGSLRSGA"
     gene            complement(3848..4843)
                     /locus_tag="VF182_18835"
     CDS             complement(3848..4843)
                     /locus_tag="VF182_18835"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439184.1"
                     /translation="MLRVKQLMIGFAWAVAFAPPAVGQSLPQVRAAYTSIGIQFDPVY
                     IMKELELPRKHGVDAEILFVPVSSRAVQAALAGEIQFITSAGVANINANVSGGDFVGL
                     TATLNTFVFKILGSPEIKKPEMLKGKKVGISRLGGASDFSIRYALTRWGLVPDKDVAI
                     IQVGGEQEEFLALQNKAVDAVVLSEPFATLAQRQGSTIVADLSQLGVAYSMHGFGARK
                     SFIQPNRDITVRFMKAYLEGIYVFKTNKDVALNVLKKYTRLDDLSLVQVAYDEMSQRL
                     IRRVPYPDREGIQTIIDQLAKTRPQMKTLNPGDFIDPSILKEIEDSGFIKKLYGN"
     gene            complement(5382..6071)
                     /locus_tag="VF182_18840"
     CDS             complement(5382..6071)
                     /locus_tag="VF182_18840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6439185.1"
                     /translation="MKRGLNRCLGALLIVSLWLAGCAEWGYRQMNDPARDAWQKPKQV
                     IERLGIEPGARLADLGAGGGYFTWYLAAAVGPQGKVYAVEIDDTALGIIEQERKSRGV
                     TNVVPIRAEPGDAKLPEPVDLVFSCDTYHHMEDRVAYFQSLARYLKPDGKVAILDFHA
                     EGFFSGLLGHGTTKQQVRQEMESAGYRLAADYDLIDRQHFQIFSRTDLAGAATLRPSH
                     GARLYPLSMAR"
     gene            complement(6672..7679)
                     /locus_tag="VF182_18845"
     CDS             complement(6672..7679)
                     /locus_tag="VF182_18845"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439186.1"
                     /translation="MKKPVNMTKIFTRCLVAAVVLSIMFFGREPAVAQSLQVVNVAIP
                     AKSFQMVIYPIAQQKGYMREEGIDLRVIYIAPTTSIQAMLSEDVQFTGAGSSALVSIA
                     RANAPLKVVLATNDRVLQWLLTRPDITNPKDLKGKRIATTGVAAIATFMLKQILNKHG
                     LDGNKDPLYLDVGQGNQLTALLAGTMDAAVLSVEQRYVGLDKGMREMFFFGNEVKNSW
                     GTLATTDKLIKENPKMISSFVRAALKSLRYMRQDKEGTVAAMMKFAGVSQQQATRVYE
                     DLIGTFTRNGAVDEEAQKNDLSIIRQVVNAAEAVPNNRAYDFSFALQAEQQLNKTGWK
                     P"
     gene            complement(7756..8850)
                     /locus_tag="VF182_18850"
     CDS             complement(7756..8850)
                     /locus_tag="VF182_18850"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6439187.1"
                     /translation="MTGIIDADTHISESEAMWSLLDKEMYHRRPVLLKAPEDTLYGPR
                     NAFWLIDGNIFPKPNGKGSFRLITPSASKLESSRGDIHLAYREMTDIPGRLRDMDKLG
                     VEKQVVFPTLFLIYITDDTALDVAMARAYNRFLAQACAKSGGRMRWVVVPPLHSVEES
                     VKEIKWGKDNGAVGVFFRGIEGYRTLDNPYFNPIYHAASDTDLPVLVHTGAGCPLFLN
                     LFDVERNHTFGHSRVQPLFAFRDLIANKIPEQFPRLKFGFIEASAGWVPFMMHIIKRL
                     FRDKWKFSSDQDMFREYRIFVACEADEDVSYIARYTGEDHLLIGSDYGHQDPSEERQL
                     VAAMRAREDIPRALTDKIFFDNPKRLYPLN"
     gene            complement(9057..9956)
                     /locus_tag="VF182_18855"
     CDS             complement(9057..9956)
                     /locus_tag="VF182_18855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439188.1"
                     /translation="MKLAKLNVMYGHLEGTDGKFARDPSGYLSIDEGIYERHELEVSW
                     RHVQGTEARYQQLESGAAHISMVVGRASLQHFLDTKKTRLLGCVMNSCSYYLIAADAI
                     KTMGELRSKTIGCREGPAQGVAFGRLLEDRAGLKLGKDVTLQFTDSDQDAFRLLLGGQ
                     IQAAWLPRPYGFIAEEKGFVRLNEGSDMVDDPLPITMETTAWLARERVEDFSRFIQAH
                     REGIRNLRNHRDEAESLLKTRFGHSPELAAKTFDEYLICMDDRLTVDFKHLDKLVAQI
                     APGIPGAAQQIASEWIIPGALRK"
     gene            10121..10546
                     /locus_tag="VF182_18860"
     CDS             10121..10546
                     /locus_tag="VF182_18860"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6439189.1"
                     /translation="MEVKDVSALAKERRKGQRRLDLFRRENLETWILYFPPGDVQRMH
                     NHPSDRTYYVLSGRGVMKGLKETHELAPGQIISIPAHEYYEGSNPHDEPMILLGNSHK
                     ATPEDVAKAGGQRNEIDDKTGKRIIYQHGGGRDEGVLAD"
     gene            10845..13973
                     /locus_tag="VF182_18865"
     CDS             10845..13973
                     /locus_tag="VF182_18865"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX6439190.1"
                     /translation="MNSKPIELTKISSSTSWLSAVLTKRWLKLWLWFVVSLLLLAICI
                     VIEFKTSIVQAWIFTRTNERLSYELAPGAASSIAFPAPAPFDDRRGYSKLADFQTRLL
                     SQGFQVSQQARQSETMVNLISQGISPPYIEPPETGIDIRGATGEPLFRHAQSEFLFDK
                     IDEIPPVLIKTLLFLEDRDLDSPNFSRQNPAVEWDRFFKAALLYLGSKLYLDVPVQGG
                     STLAVQLEKFRHSAHGRTDSPLDKFRQIVGASLKAYRAGPNTRAWRDRIIVDYFNTVP
                     LAAAPGYGEIHGLGEGLYAWFGMQLADALRALQTPGITIPKVRVYKHILTLLISVRAP
                     SVFLVEERGSLEHKVNQFTQLLAREGIIDGDFAAAVEEMPIKFLSHGPVPPQPSSGRN
                     KAANAIRMTMTGLLGVTNLYDLNRLHVEVESTIDVPLQKKVVEFLQSLTLPETIKAHG
                     LNGERLLENSDPKRVIYSFLLIEPTPQGNFVRVQADNLAAPFDFNKSVKLELGSTAKL
                     RTLTHYLELIAELHKELSPLNSKELGQKLRYARDPLTSWAIEALKTEPGLALQPFLER
                     AMERRYSASPFEAFFTGGGLHHFENFDREDNGKILLLREAFRNSTNLVFIRLMRDVVS
                     YHRARLAYNADAVLSDPDNAARQAMLARIAEEESRAALRRAYQNFRGQTPEQITARLL
                     GSKRNAERRLAILFFAWRIGADEQGLAAWLAQNQYQATAEVVGKLFRAYRNPRLTLVD
                     YAYLLSLHPLDLWCASEFRSNANLSWEELYNKSGEARRSGSAWLLTPRNRKAQDLRLR
                     IRFERDAFARMTPYWQRLGFPFKTMVPSYATALGNSSDRPVALAELIGILVNDGLRKR
                     SVSLNKIHFARSTPYETVMEPEPRAGVQVLAPEIARTVRKAMADVVERGTARRLNGVF
                     RLADGQLLTVGGKTGSGDNKFETFNRYGGVISSRATNRTAAFVFYIGDRYFGVITAYV
                     QGREAADYRFTSALPVTLMKILAPTLTANIDATTTRTSDDRGNGIKANTTPRKISFDG
                     PSVIPAPVSVAH"
BASE COUNT         3174 a         3855 c         3796 g         3252 t
ORIGIN      
        1 agggccctcg ttccgcgcgg gtgatccatt cgagcatcac cgcgccggaa gcggaggata
       61 ctcgactcgg taagcctatg gcgatgcgca tactttcctc catgctctga tttgaaggtc
      121 aggttacaag aattttctct ccgattgtta aagccgaggt agagagaagc gatcggaatc
      181 tcttctcgcc gtattctccg ttgcctcctg ccggatctct tgaccggctt cgcgtgttta
      241 gcgcgccgat gtgtaccagc cgaaatcccg tttaggtttc tggatacaat tctaaactac
      301 ttggcgtcaa gcttggcgcc gttgccgctg cggcgtggcg cttggaaacc gcctcgcaaa
      361 atagcaagat gggatgcgcc gggagtcttg cagaagacgg gtaacttggg cgaaggcttt
      421 atgatcccga tgatgaatat ctacgacacg attcgcacga gtttgcactt caataagttc
      481 gttgtcgggg agctgctttt cgtcgagtac aagtgcccca ttgagcagga agttgccggc
      541 gtgtggacgc cgatggattg ttttgttcac gtgctgagcg gcaaaaagac ctggcgcacc
      601 acggacggaa cattgacagc cgaaaaaggc cagaccttgt ttttcaagaa aggcgctgcc
      661 gtcgtctacc aggatttcaa ggacgatttc tgcgtcatgg actttttcgt ttcggataat
      721 ttcatccgcg acgtcgtcag ggaagtgtcc ggccaattgc gcgggcggcc tgctggcgac
      781 gcgttccaaa aagccgccat tctagtaaac ggcgacgtga ctttgtcggc ctattttcaa
      841 tccatgctgg cctatttcac tgggacagca aagccggccg aaacattgtt gacgctcaag
      901 ctcaaggaat tgatcgtgag catcctgttg gggggcaaaa accccgagct cgccggttac
      961 tttcaatcgc tgctcaaaag cgatgcgcct gtgctgtcgc aaatcatgga ggcgaatttt
     1021 tcttacaacc tggcgctgga agatttcgca aggttatcgc atcgcagcct gtcaaccttt
     1081 aaacgcgact tccgcaagta ctacaaccag tccccaggca agtggttgtt gcaaaagcga
     1141 ctggactact cagccgtgct gctaaaaaat cccgcgctca acgtttcgca agtggccttg
     1201 gattgcggct tcgaagacct gtcgcatttc agccgggcgt tcaaaggaaa atttggcacc
     1261 tccccggcta attaccggaa aggaaactcc cggtcacgcc ttctcgcggg ttagtgaaag
     1321 tgacctcttg caggctgctc aaaaaagatc cagaggggag gcgcgcggtt tcaaaactga
     1381 aagcggaaga tgaaagctaa aactttagat gcatttaacg tgagttcgtt gggtctcagg
     1441 atttcagctt tccgttttcc tctttcagct tttaatcagg gcttttcagc aggcccttgc
     1501 aggttgctca aaaagttcag atgcgaggcg cgtgagaatg gacgagcgta ggcgtactca
     1561 tgcggtacgt cggagcgaat ccatcggagc gcaacgaagc aggtgggctt ttttcagcaa
     1621 cctgcatgag aaagagctgc tcagtcaaac ttctggcctc caaaacaaag cattgttttc
     1681 tgccccagtt tatctttggc agcagaataa gcctttaagg aggcaatcat gaactattcg
     1741 aacgcatctc attggaccga ggttgctcat agcctgggcg gagaattcgc cacccgcgcc
     1801 gcggcccacg atgagcagga cacatttgtc agcgctaatt acgcagcgct caaggagcgg
     1861 ggcgtgttct ccgcggcggt tccgcaagaa ttgggcggcg gcggagcctc gcaccgtgag
     1921 atctcggaca tgctgcgcac cctggcccaa tactgcggct cgacggcgct agcgctctcg
     1981 atgcaccagc atctgatcgc cgctacagtg tggaagtatc ggcaaggcca aggcggcgaa
     2041 cccttgctca agaaggtcgc cgaaaaccag ctcgtgcttg taagcacggg cgccggcgat
     2101 tggctggagt ccaatggctc gatgagcaaa gccgacggtg gctatgtcgt cagcgcccgc
     2161 aaacatttcg ccagccagtc ggcggtcggg gacatgctgg tgacgagcgc cgtgttcatg
     2221 gacgaggtgt tgcacttccc ggtaccgatg aacgcgccgg gagtctcggt cttgaacgat
     2281 tggcgcgcgc tcggcatgag aggcaccggt tcgcataccg tcaaactgga aaacgtcttc
     2341 gtaccggagg ctgccattgc tgtgcggcgg ccgcgcggcg cctatcaccc agtttggaat
     2401 gttgtgctga ccttggcgat gccgctgatc atgtcggtct atgtgggcat cgcccagaag
     2461 gcggtggagt tgtccgtgag tcatatccgg cggaaaaaaa acaggaagcc gcaggtgccc
     2521 tctttgatcg gtgaaatgca caacgatctg gtggccgctg agattcagct gaatgatatg
     2581 ctgcgcatcg ccaataatta cgagttcgaa gccatcgacc aaaacggcca agacattctc
     2641 gctcgaaagg tcaacgtcgc caacgcaacc ataggcgttg tcaccaaggc gatggaaatc
     2701 gtcggcggtg agggcttgta ccgggatttc ggattggagc ggcttttccg cgatgttcaa
     2761 ggcgcgcgct atcatccgtt gccggaagcg gagcaggtaa agttttcggg agaatatcta
     2821 ttgcgcgatt gatcgatctt gaacgttcca tatgaataag aaagggacca gggtaggggt
     2881 ggatcggtcg agcaagttat acgcactcgt atcctgagcc ttcccattaa cttcaagtgg
     2941 tcaagagcgc gaagacatgc ccgtgagggt tgtctcacat tggttaactt attcggcgac
     3001 aatacttcag cacacttgca ccgaacagca tgtaacgttc aaaacacctt cgccaccgaa
     3061 accgtagtat tctgcttaga agggctgcca ggctgctcaa aaagactcag atgggaggcg
     3121 cgccgtttca aaactgaaag cggaaagatg aaagctgaaa cttcaaatgc atttaacgtg
     3181 agtttgttgg gttctcagga tttcagcttt ccgttttcat cgttcagctc taaaagcagg
     3241 tctttcagcg gactgctacg cgcctgaccg taggctgcca tagagccgct cgggggtaac
     3301 caatattatg cttcgctctt catcccgcat ttgccgggca aattcctcgt ctgttccctt
     3361 gggggctcgg cccatagaaa tgtaaaggtc tctcagacgc gtgatgtgat gttgggaatc
     3421 ttcccatggc tcgattgacg ccgggccctc gacggtgacg tagcgccggt tgtcgagctt
     3481 gatgacggtg agtgttgccc ggccggtccg ttggatgttc ttaagcttga tggtgcgcgg
     3541 ccgcgacgcg aatccgactt tgccatcgag caccgccgtc gacacgacgg tcgactgcgc
     3601 gcgaccctcc gtcgtgatgc tggtcgcgac acccgtgtgg ttctcttgta acagagggag
     3661 tgcttctttc cattccatgt attcgttcct ccgatgtgaa gtgttagcgg ctcagccgcg
     3721 ctgaacttgg cagctatagc gctgctcgag gcgatgagtc aagctgatgc gagtctcgtc
     3781 gaccggaaag ataatcaatg ggatctcgtg gctggattcg attaccctat gctcgtgaaa
     3841 acctcggtta atttccgtag agttttttga tgaagccgct gtcttcgatt tctttcaaaa
     3901 tcgacggatc gatgaagtct ccggggttga gggttttcat ctgcggtcgt gttttggcga
     3961 gttgatcgat gatggtctga attccttcgc ggtccggata agggacccga cgaatcagtc
     4021 gctgcgacat ctcgtcgtaa gccacttgca ctagggacag atcgtcgagc cgcgtatatt
     4081 ttttcagcac gttcaaagcg acatctttgt tggttttgaa aacatagatt ccctcgagat
     4141 aggccttcat gaatcggacg gtgatgtcgc gattgggctg gatgaagctt tttcgcgcgc
     4201 cgaaaccatg catcgagtaa gccacgccga gctggctcag gtcggcgaca atagtcgagc
     4261 cctgccgctg cgccagtgtc gcgaagggct ccgataaaac gaccgcgtcc accgctttgt
     4321 tttgcagcgc cagaaactct tcctgctcgc cgcccacctg aataatcgcc acgtctttgt
     4381 cgggaaccag accccaacgc gtcagcgcat agcggatcga gaaatcgctg gcgccgccca
     4441 gccgggaaat accgaccttt tttcccttta gcatctcggg ctttttgatc tccggactgc
     4501 caagaatctt gaacacgaaa gtattgagag tcgcggtgag accgacgaag tcgccgccgc
     4561 tgacattagc gttgatattg gcgacgccgg cgctggtgat gaactgaatc tcaccggcca
     4621 acgccgcctg aaccgcgcgc gagcttaccg gcacaaaaag aatttcagcg tcgacgccgt
     4681 gtttccgcgg cagctcgagc tccttcatga tgtaaacggg gtcgaattgg atgcctatcg
     4741 aggtgtaggc ggcgcgcact tggggcaggc tttgtccaac cgctggtggc gcgaatgcca
     4801 ccgcccaagc aaaaccgatc attaattgtt tcactctcaa catggttcct ccgtactcgg
     4861 tcaaaacgac cgggtatcac cggagctgcc atcgcgtcaa gacaacgtct ttcatgagaa
     4921 tcggcttccc gttgctgctc tgcttccttt cgccaaacga gcaatccgcg ttcaactttt
     4981 cggcagccgg cctgtcgatc atgatctttg gtctgcggag aatctgatgc gcctgcgctt
     5041 ggagctcgga tgcagccgct cgcctccaaa ccgcgccccg ctttcgccag ctcctcgttg
     5101 aattttcctc atgtcggtaa gaagctgttg cctcggcatt atccctgctg ccgattattt
     5161 gttcgcttta ccaaaaatca tgaatcgcat tgttgttcct ccttcttcta atcagaggcg
     5221 cgcgcccgga tctcctcgaa ttctcaaccg gatctgttga atcgacgaac gagaagtcgc
     5281 caaatggaaa ccccgtcgcg agcaaattca aaattagata accgcgcgag atttaccaac
     5341 acttgccaat cgaattcgta atcgcctgct tgaagcgaat atcagcgcgc catgctcagc
     5401 gggtataatc gggcaccatg cgatggccgc aacgtggctg ccccagccaa atcagtgcgg
     5461 gaaaagatct ggaaatgttg ccgatcgata agatcataat cggccgcaag ccggtaaccc
     5521 gcggactcca tttcctgccg cacttgctgt tttgtcgttc catggccgag caagccggaa
     5581 aaaaatcctt cggcgtgaaa atccaggatc gccactttgc catcgggttt gagatagcgt
     5641 gccagtgatt gaaagtatgc tacgcgatct tccatatgat ggtaggtatc gcaactaaag
     5701 acgagatcta caggctcagg aagtttggcg tcacccggct cggcgcgaat aggcacgaca
     5761 ttcgttacgc ctcgagactt cctctcctgc tcgattattc ccaaagcggt gtcgtcgatt
     5821 tcaaccgcat aaacctttcc ttgtggtccg accgccgcag cgagatacca ggtgaagtag
     5881 cccccgccgg cgcccagatc cgccagccgc gctcctggtt cgatgccgag cctttcgatg
     5941 acctgtttag gtttttgcca ggcatcccgg gcaggatcat tcatctgccg atagccccat
     6001 tcggcacaac cggcaagcca tagacttacg atcaacaatg cgcctaaaca cctgttcaac
     6061 ccgcgcttca tcgtctctcg ttttaataat gaaagctcca tcaggataga ctatatcgag
     6121 cgaaagcgga gtcaaactct ctagatattg actgccggcc tctcgctacg atgatgctcc
     6181 ataagcgcga ggacaaatga tcaggctccg cgcgattccg gattggatcg ctacggtcga
     6241 ggggggagaa cgaagattat cgcggacagc gtggcgcatt catgcggttc atcactgaag
     6301 ctgccatatg aatgctgaag tggttcatcc acgttcacaa acgggctttg tgcggttttt
     6361 ttgacgcccg gatgaaagta gagaacggtg aaaaagaccg ccgagaaaaa gcgagttaat
     6421 gattccaaat accactagaa ccggatccgc agactgcagg ctcgacggca gcatggcgag
     6481 gacagagcac cagacgccga ttacccagac cagactgata tctcccgaag aacgccgccg
     6541 gaaagatccg gagtatcaga ggaatattga agagcggcat gacaatgccg ctgaacatgc
     6601 caacgatttg aataatccgc ataagcttta tggatcaacc agtgtatata gagctcgatc
     6661 gcgaatcgtg tctacggctt ccaacccgtt ttgttgagct gctgctcggc ctgcaaggcg
     6721 aaactgaaat cgtaagctcg attgttcggc accgcttcgg cggcgtttac cacttgccgg
     6781 atgatgctga ggtcgttttt ctgcgcttct tcatcgacgg cgccgttgcg agtaaaagtg
     6841 ccgatcaagt cttcatagac acgggtggct tgttgttgtg aaacaccggc gaacttcatc
     6901 atcgctgcga cagtcccttc cttgtcctgg cgcatgtagc gcaaggactt caaggcggcg
     6961 cggacaaagc tgctgatcat cttgggattt tccttgatta acttgtcggt ggtcgccaat
     7021 gttccccagg aattcttcac ttcattgccg aaaaagaaca tttcacgcat gcccttgtcg
     7081 agaccgacat agcgctgctc gacgctgagc accgccgcgt ccatggtgcc cgccagtagt
     7141 gcagtcaatt gatttccttg gccgacgtca agatagagcg gatccttatt tccatcgagc
     7201 ccgtgtttgt tgagaatctg cttgagcatg aacgtcgcaa ttgccgcgac tccggtagtg
     7261 gcaatccgtt tgcccttgag atctttcgga ttggttatgt cgggccgggt caggagccac
     7321 tgcaggacgc gatcgttggt ggccagcacc accttgagag gcgcattggc tcgagcgatg
     7381 ctcaccagag cgctcgaccc cgccccggta aactgaacat cctcgctgag catggcctgg
     7441 atgctggtgg tcggtgcgat ataaatcacc cgcaggtcga tcccctcctc cctcatgtag
     7501 cccttctgct gggcgatcgg atagatcacc atttggaaac tcttcgcggg aatggcgaca
     7561 ttgacaacct ggagcgactg agcgacggct ggctcccggc caaaaaacat tatcgaaagg
     7621 actacagcgg caacgaggca ccgggtaaaa atctttgtca tattaaccgg ctttttcatt
     7681 cactactccg attgccgccc gctctctgat gaaagccggg cggtagaaac gatgacctgc
     7741 gacctgagac gcgactcaat ttagcggata gagccgcttg ggattatcga agaagatctt
     7801 atcggtaagc gcgcgcggaa tatcttcgcg agcgcgcatc gcggccacga gctggcgctc
     7861 ctcggaagga tcctgatgcc cgtagtccga gccaatcagg agatgatcct cgccggtata
     7921 tcgagcgata tagctcacgt cctcatccgc ttcgcaggca acgaaaattc gatactcgcg
     7981 aaacatgtcc tggtccgagg aaaacttcca tttatcacgg aataatcttt tgatgatgtg
     8041 catcatgaac ggcacccatc cggcggaggc ttcgatgaag ccaaatttca ggcgcggaaa
     8101 ctgctcggga atcttgttgg cgatcaggtc acgaaacgcg aacagcggct gcacccggct
     8161 gtggccgaag gtatggttgc gctcgacatc gaagaggttc aaaaatagcg ggcaccccgc
     8221 gccggtatgg actaagaccg gcagatccgt atcgcttgcc gcgtgataga tcgggttgaa
     8281 gtaaggattg tcgagggtcc tgtatccttc gataccgcga aagaaaactc ccacagcgcc
     8341 attgtctttg ccccatttga tctctttcac ggactcttcc accgaatgca gcggcggcac
     8401 caccacccaa cgcatgcgcc cgccggattt ggcgcatgcc tgcgccagaa atcggttgta
     8461 agcgcgcgcc atcgccacgt ccagggctgt gtcatcggtg atgtaaataa gaaacaaggt
     8521 cggaaatacc acctgcttct cgacgccgag tttgtccatg tccctgagcc ggccaggaat
     8581 atcggtcatc tcccggtaag ccaaatggat gtcgccgcgg gaggattcca gtttggacgc
     8641 cgacggggta atcaaacgga aactgccctt gccattgggc ttgggaaaga tgttgccatc
     8701 gattagccag aaggcattcc gcggtccgta caatgtatct tccggagctt tgagcagcac
     8761 cggccggcga tggtacatct ccttgtcgag caagctccac atcgcttcgg actctgagat
     8821 atgagtatcg gcatcgataa tccctgtcat gagcggcctc cttctctaag ctaattaatg
     8881 tgtagccggt aaaaattttt gttcaaccgg tgcagccggt ttaatatgtt tcaacgcgac
     8941 cgaggcggta ttcaaacgtt caacggttca acgtcagaca gcgctcgact tttctgtcgc
     9001 ctgcgccctg gaatcggcgc atattgtgcg cccgctgaac tgttaaatcg ctcttatcat
     9061 ttccgtagcg ctcccgggat aatccattcc gaagcgatct gctgcgcggc gccaggaatc
     9121 cccggcgcaa tctgggcaac gagtttgtcg agatgtttga aatccaccgt gagacggtca
     9181 tccatgcaaa tcagatattc gtcgaacgtc ttggctgcca actctggcga atgcccgaag
     9241 cgagttttta agaggctctc agcctcatct cgatgattgc gtaaatttcg gatgccttcg
     9301 cgatgcgctt ggatgaaacg ggagaaatct tcgacgcgct ctcgtgctag ccaagccgtc
     9361 gtttccatgg ttatcggcag cgggtcatca accatatcgg atccttcgtt gagcctaacg
     9421 aagccctttt cctccgcgat aaatccatac gggcgcggca accatgccgc ttgaatttgc
     9481 cctcccaata aaagccggaa agcatcctga tcactatcgg taaactgcag agtcacatcc
     9541 ttaccgagtt ttagtcctgc tctatcttcc agtaatcgcc caaacgcaac accctgcgcc
     9601 ggtccttccc ggcacccgat tgttttactt ctcagttcgc ccatcgtctt gatcgcgtcc
     9661 gccgcgatta ggtagtagct acagctattc atgacacaac ccaacaggcg ggtcttttta
     9721 gtatcgagaa agtgctgcag cgatgctcga ccaactacca tagaaatatg agcggcgccg
     9781 ctttcgagtt gctgatagcg agcctccgta ccctgcacat ggcgccatga aacttcaagt
     9841 tcatgtcttt cgtagatgcc ctcatcaatc gacaggtagc cagacggatc gcgggcaaat
     9901 tttccatcgg ttccttcaag gtgcccatac atgacgttga gctttgcgag tttcattgga
     9961 gtgctcctgt ggcagtcaat tgaactttct atagtacgga tgggtgcctc agacaaataa
    10021 attgctggcg agcgtcatct tgacagccag tctcctacgg gaatagagtc gcggccaaag
    10081 cagtgccggc atactatttc aatcagaaag ggaggccatc atggaggtga aggacgtttc
    10141 cgccctagca aaggaaagaa gaaagggaca gcggcgcttg gatctgtttc gcagagaaaa
    10201 tctagaaaca tggattctct atttcccccc gggagatgtg cagagaatgc acaatcatcc
    10261 atcagatcgt acctattacg tgcttagcgg ccgcggcgtg atgaagggcc ttaaagaaac
    10321 ccacgagctt gctcccggcc agatcatcag catccccgcg catgaatact atgaaggatc
    10381 aaacccgcac gatgaaccga tgattctctt gggcaacagt cacaaagcaa cgcctgagga
    10441 tgtcgccaaa gccggcgggc agcgcaacga aatcgacgac aagaccggca agcgaattat
    10501 ctatcagcac ggcggtggta gagatgaggg agtgctggcg gactgaacct gttggatggc
    10561 tagggttgta caaaagtccg gcggtgatga gctgtcggac ttttttattt cgtaattgat
    10621 cctaacgtcg aatgacctcc agctacattt taggtgcgaa ttaccaagca agcgtttcga
    10681 aagcgatacc gctcttagat aaaattacat tttcctcctg aatcaggcca acgacagtag
    10741 gcaccctcga aaacagttcc gaggtgtcga gcgcatcctc atcaagcgtc aacttcgagt
    10801 taggattttg tagcaattat gctatcattc cttggctcac ccaaatgaac agcaaaccca
    10861 tcgagctaac taagatttcg tcgtccacgt cctggctttc ggcggtgtta accaaaagat
    10921 ggctgaagtt atggttgtgg tttgtggttt cattgctgtt gctggcgatc tgcatagtca
    10981 tcgagttcaa gacctctatc gtccaggcgt ggattttcac gcgcaccaac gaacgactat
    11041 cttacgagct ggcgccagga gcggcatcga gcatcgcgtt tcctgcgccc gcgccttttg
    11101 atgatcgtcg tggttactcg aagttggccg atttccagac tcggcttcta tcccaaggct
    11161 tccaggtcag tcagcaggca agacaatcag agaccatggt caatctgatc agccaaggca
    11221 tttcgccgcc ttatatcgaa ccgccggaaa ctggcatcga catacgaggc gccaccggcg
    11281 aaccgctctt tcgccatgcg caatccgaat tcttgttcga caaaatcgac gagatccccc
    11341 cggtattgat caaaaccctg cttttcctcg aggatcgtga tctcgacagt cccaacttct
    11401 cgcgtcaaaa tcccgcggtg gaatgggacc gctttttcaa agcggcgttg ctctatctgg
    11461 gttcgaaact ctacctggac gttccggtcc aaggtggcag cacgttagca gtgcagctgg
    11521 aaaagtttcg ccattcggcg cacgggcgca ccgactcgcc gctcgataaa ttcaggcaaa
    11581 tcgtcggcgc cagcctaaag gcttaccggg cggggcccaa cactcgagct tggagagacc
    11641 gaattatcgt tgattatttc aataccgttc cgctcgccgc tgcacccggc tacggtgaga
    11701 ttcacgggtt gggggagggg ctttatgcct ggttcggcat gcagctcgca gatgctctcc
    11761 gcgcgctcca aacgcccggg atcactattc ctaaagtccg tgtctataag catatcttaa
    11821 ccttgctcat ctctgtgcgc gcgccctcgg tattcctggt cgaggaacgt gggtcgctcg
    11881 agcacaaagt gaaccagttt actcagttgc tggcgcgcga agggattatc gacggtgact
    11941 tcgcggcagc cgtcgaagag atgccgataa agtttttgtc ccatggtccg gtgccgcccc
    12001 agccttcttc cggaagaaac aaggcggcta acgcgatacg gatgaccatg accgggcttt
    12061 taggcgttac caacctatat gatctgaacc gattgcacgt cgaggtcgag agcaccatcg
    12121 atgttccctt gcaaaaaaaa gtcgtcgaat ttctgcagtc tttgactctt ccggagacga
    12181 tcaaggctca cggtttgaat ggtgaacggc tgttggagaa ttccgacccg aagcgggtca
    12241 tctattcttt cctcttgatc gagcctacgc cccagggaaa ctttgttaga gttcaggccg
    12301 ataacctggc cgcgcctttc gacttcaaca aaagcgtcaa actggaattg ggaagcacgg
    12361 ccaaattgcg aacgttgaca cattatctcg agctgatcgc cgaactccac aaagagctct
    12421 cacccctcaa ttcaaaagag ctgggacaga aactgcgcta tgctcgtgac ccactcacca
    12481 gttgggccat tgaagcgttg aagaccgagc ccggtcttgc cttacagccg tttctcgaac
    12541 gcgccatgga gcggcgttat tccgccagcc cttttgaagc attcttcacc ggcggcggtt
    12601 tgcaccactt cgagaatttc gaccgcgagg acaatgggaa aatattgctg ctgcgggaag
    12661 cgttcaggaa ctccacgaat ttagttttca ttcgcctgat gcgggacgtg gtgagttatc
    12721 accgcgcgcg cctggcttat aatgccgatg ccgtgctcag cgatcctgat aatgcggctc
    12781 gacaagccat gctggcgaga attgccgaag aagagtcgcg cgctgctctg cgccgcgcct
    12841 atcaaaattt tcgcggccaa actccggaac agattacggc gcggctgtta gggagtaaac
    12901 gaaacgccga acggcgtctg gccatcctgt tctttgcctg gcgcatcggc gccgatgagc
    12961 aagggctagc ggcatggctg gcccagaacc aatatcaggc aaccgctgaa gtggttggca
    13021 agctgttccg ggcttatcgt aacccgcggc taactttggt cgattatgct tacctgttat
    13081 cgttacatcc gctggatctc tggtgtgcta gcgagtttcg cagtaacgct aatctttcct
    13141 gggaggaact ctacaacaaa agcggtgagg cccgccgctc gggatcggct tggctgctca
    13201 cgcctcgcaa ccgcaaggcg caggatctcc ggctgcgaat acgttttgag cgcgacgcgt
    13261 ttgcgcgcat gactccttac tggcagcggc tcggatttcc gttcaaaaca atggtgccgt
    13321 cctatgctac cgccttgggg aactcctcgg atcggccggt ggccctggcg gagctgatcg
    13381 gaatcctggt aaatgacggg ctacgcaagc gcagcgtcag cctgaacaag attcacttcg
    13441 ctcgcagtac cccttatgaa accgtgatgg agccagagcc gcgagcgggc gtacaggtgt
    13501 tggcgccgga gatcgcccgt acggtgagga aagcgatggc cgatgtggtc gagcgcggca
    13561 cggcacggcg tctcaatgga gttttcaggc tcgccgacgg gcaactgctc acggtcggcg
    13621 gtaagaccgg atcaggcgac aacaagtttg aaacgttcaa tcgttacggc ggcgtgattt
    13681 cctcgcgggc aaccaatcgt acggcggctt ttgtctttta catcggtgac cgttacttcg
    13741 gtgtgatcac cgcgtacgtc caggggcgcg aggccgccga ttatcgcttt accagcgcgt
    13801 taccggtcac gttgatgaaa attcttgcac cgaccctgac agccaatatc gacgcgacta
    13861 caacccgaac ttccgacgat cgtggaaatg gcatcaaagc aaatactacg ccgcgtaaga
    13921 tcagctttga tggtcccagc gttattcctg cgcctgtaag cgtcgcccat tagcacatct
    13981 cacagatggc gcgagcacaa tgcttacgcg tatggccacc atccagccga gctccgagcc
    14041 acctatccag attcatattt cttaatcttc cttgcaa
//
LOCUS       DASUVS010000354         7794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1462169, whole genome shotgun sequence.
ACCESSION   DASUVS010000354 DASUVS010000000
VERSION     DASUVS010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462169"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            355..579
                     /locus_tag="VF182_18870"
     CDS             355..579
                     /locus_tag="VF182_18870"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX6439191.1"
                     /translation="MAGRQLRGVDGEEAVKGFVKAGGVRRAGKGDHVNIKMPNGQLVT
                     IPIHRELKIGLLKAAIRKAGLTEEQFLDLL"
     gene            592..813
                     /locus_tag="VF182_18875"
     CDS             592..813
                     /locus_tag="VF182_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX6439192.1"
                     /translation="MQYTVIVHNADEGGYWVEVPSLPGCFSQGETIDEAMNNVKEAIE
                     LHIQGLRDEGQEVPTEKDLVIGRVEVKAA"
     gene            822..1304
                     /locus_tag="VF182_18880"
     CDS             822..1304
                     /locus_tag="VF182_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439193.1"
                     /translation="MSIYATLWWLKFPRYGDEYIGCEWIRVSAQGVPAHVGTPTQGFG
                     YEDGDPYAEFLPPAVYVNAEGDSEFMRAIVIVTEETQKGTARSPQEYVNSILVLSGRE
                     YASISFVELHTRICDALRGSGPAVVAQSFTVDDEMQLVLSDGRILDTGKQGRGSRQFD
                     "
     gene            2071..2322
                     /locus_tag="VF182_18885"
     CDS             2071..2322
                     /locus_tag="VF182_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002739985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HEX6439194.1"
                     /translation="MSEITLSIPDETLVALKVKPDQLGAEIRLAAAVKLYELGRISSG
                     AAAKLAGIPRTLFLMKLADYGVDTFKLTEQELQQEHRLA"
     gene            3399..4418
                     /locus_tag="VF182_18890"
     CDS             3399..4418
                     /locus_tag="VF182_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEX6439195.1"
                     /translation="MRNGKIRIGIMGLGQIGRHLYHLAREIDDIEIVAVADIGKPEII
                     QYLLNSDGVDEAPCELQDNYLINEKFRTRMLQLARPDEVPWDVLNVHCVVDATGRYRN
                     RRDMEAHLRAGAPRVILASLPAESIDRIVVPGINEGSAAAADKMISAGSATTNAFALL
                     LQSLDQALGVDCASMTTVHAYSSDQSLQNYAHKDFRRSRSAAENIIPNNNESARWVEL
                     VLPKFEGKLSGYALNVPVQRGSLLDVNCVMQAGTVTAEQVNGAMRAAAADHPTLIGVT
                     DDPIVSSDVIGCRQSLLFDSLATIKAGKRIIKVLAWYESLGHACRVLEVVRHYGRLDA
                     IGGGR"
     gene            4415..5425
                     /gene="gap"
                     /locus_tag="VF182_18895"
     CDS             4415..5425
                     /gene="gap"
                     /locus_tag="VF182_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX6439196.1"
                     /translation="MRVAINGFGRIGRSVFRILDKRADAELVAINDLFDHDALRYLLN
                     YDTVMGRFDGALRLEGNELVTSHGRTRMLREKEPLKLPWRELDIDVVIESTGIFTTRE
                     QLAKHLDAGAKRVVLTVPAKDEIDYTVVIGVNDDGLKPEHRMISNASCTTNCLAPLAK
                     VLHDSFGIVEGIINTVHAYTNDQRLADVPHSDWRRSRAAAENIIPTSTGAAKAVGEVL
                     PELKGRLDGIAARVPVPDGSVVDLFVELQKSVSAEDVHGAIKSAAESKRMKGILQLMA
                     TPVVSSDVIGNSHSSIFDPAFTSVTGGRYLKCLSWYDNEWGYSNRVCDLLGLIARWEK
                     RE"
     gene            5839..6792
                     /locus_tag="VF182_18900"
     CDS             5839..6792
                     /locus_tag="VF182_18900"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439197.1"
                     /translation="MTIRISLVFSAIVFTLSAGAPANGAEKLRVAYPTLGPGSTPSWV
                     THDAGFWNKSGLDVELILLSGGARMLPALVSGSVEIILGSDTGVTLANLQGANLVRIG
                     VTMNSLGYSLVTQPGIRSIQDLKGKVLGTGRGRDASYARLVKILADNGLNPASDVKFL
                     PLGETPTGRLQAIKSGLIHGAVFTPPLDLVGSRDGLRILHKIDVPTLGGGINTTAPMV
                     RQNRGALLTFLRAYMEGIRYMAAHKTESLKVFFKYFRNPDMEALAYLYEDTVPRLAKN
                     LRPNPESVRSILDQIAIDEPRARQLSEKDHWDLTLLDQIQR"
     gene            7340..>7794
                     /locus_tag="VF182_18905"
     CDS             7340..>7794
                     /locus_tag="VF182_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439198.1"
                     /translation="MQRFAKFFNWLGALLSVFVSLLLSSVSSHSAVALSQEELINEAK
                     KEGRLVFYASGTTEQTTMFLDAFRSKYPFIKTEYFRTGKTALLNRVLKENQAKKIIAD
                     AIQSSVIELNLAKRGGALGKYIPHEAKDYPASYKDPEGFWTTIFISSNLL"
BASE COUNT         1861 a         2063 c         2166 g         1704 t
ORIGIN      
        1 actttcagtt tctgagtgaa gcttcgcatc accagtctcg gagccgactc atggatggga
       61 gagtacgatg cgtccacgag acctacccgc tctaagcgaa atgaggaagt agataaaaaa
      121 gtagcctgtc cgctttgctc ccatctaaac gccgaatcca gttcatgccg caagcttcgc
      181 gagccagtgt caggacgact ggaccgggag cgtagcttag aggcgagcag aatcgggaac
      241 cggatttcct ccgtttggga acgcgctgtg ggcatagtat tttaacgacg ttgaagaaaa
      301 acggctgctg gccgcaatgc ggctgcgcta ccggggtaat aaacgagcaa tttaatggcc
      361 gggcgacaac tgagaggcgt agatggcgag gaagcggtaa agggtttcgt aaaggcaggc
      421 ggagttcgcc gggctggcaa gggtgatcac gtcaacataa aaatgccgaa tggacaactc
      481 gttaccatac ctatacatcg agagctgaag atcggtctgc tgaaagcggc tatccggaaa
      541 gcgggattaa ccgaggaaca gtttctggat ctactgtgaa aggagcgtct gatgcagtac
      601 acagtaatcg tgcacaatgc tgacgagggc ggatattggg tggaggtgcc gtccttgccg
      661 ggctgtttta gccagggcga aacgatcgac gaggccatga ataacgtcaa agaagcaatc
      721 gagcttcaca tccagggatt aagagatgag gggcaggaag tgccgacgga aaaagacttg
      781 gtgatcggtc gagtggaagt caaagccgct tagagcgtcc catgagcatt tacgctacac
      841 tttggtggct caagtttcca cggtatggcg acgagtacat cggctgcgag tggataagag
      901 ttagtgccca aggggttccc gcccacgttg gtacacccac tcagggattc ggttacgagg
      961 acggcgaccc atacgccgaa ttcctgcctc ccgccgtcta cgtgaacgcc gaaggcgaca
     1021 gcgagttcat gcgcgccatt gtgatcgtta ctgaggagac ccaaaaggga accgctcgct
     1081 cgcctcaaga gtacgtcaat tcgatacttg ttctttctgg ccgtgagtac gcgtcaatct
     1141 cgtttgtcga gttgcacacg cgcatctgcg atgcgctacg cggctcaggt ccggcggtgg
     1201 tcgcgcagtc gtttacagtc gacgatgaga tgcagttggt actatccgac ggacgaattc
     1261 tcgatacggg gaaacaggga agaggaagcc gccaattcga ttgaataagc cgtaaatttt
     1321 cgtcatgcaa tcgccatgga gcgggcgtgc gcagttgccc aagcaagcaa cccccgcgtc
     1381 tccggcaatc tgtctcgcga tatcgatcca cggcatttct ttgccgtatt ggggggtccc
     1441 atgcgttaac gctagatggc aaaggcgggg attaaaggag acaggctact ttcatcgttt
     1501 gtgatcacgt tccctacacg ataaagccct gcgatgccga gaattcgcat gggcaagagg
     1561 ctggttttgt ttatcatgtg atcaaccgag ggaatcgtcg cgcagctatc tttcataaag
     1621 ctcaagattg ccaagccttt ctttccatcc tcgcgctagc aaaagcgcga cacccagtta
     1681 agatgttggc tttttctctg atgccgaatc actttcactt tttgattgaa gctttgcatc
     1741 accagtctct gagccgattc acacaatggg agagtacgat gtgtccacga gaccgacccg
     1801 ctctaagcga aatgaggaag tagataaaaa agtagcccgt cccctttaca tcccctcaca
     1861 gtgaagaaga ttcccaaagc cgtaatctcg cgctgggagc ggggcaagga cgactagagt
     1921 ctcaaggtgg agaatctacc gaaggcgccg cctcccaaaa gggcagatgg agttactatc
     1981 atagttagcc gcagagacgc agagcgcact gaacgaagtt atgacaacca cgggtgaatc
     2041 tggcaaaata gcgacgaatg gagaacggtt atgagcgaga tcacattgtc catcccggac
     2101 gaaacgctag tggctctcaa ggtcaagccc gaccaactgg gcgcggaaat tcgtttggcg
     2161 gctgccgtga agctatatga actcggccga atctcgtcgg gcgcggcggc gaaactggcg
     2221 gggattccga gaacgctgtt cttaatgaag ctcgcggatt acggcgtgga tacgttcaag
     2281 ttaaccgaac aggagcttca acaagagcat cgccttgcct gaaatcatct ccaacacctc
     2341 tccccttcag tatctgcatc agctcggtgt gctggatgtt cttccaaagc tagtcacaac
     2401 aggtacagtg ccgccagcgg tccaggatga gctgaccgca ggtcgtaagt tgggtctgaa
     2461 cctgccggat cttaatagcc tagattggat caccgtccgc cgtccgtcca gttccgccgt
     2521 tcttcccatg gtcaccttgg cgcgggggaa agggaagtcc tcgccctggg gttggagacc
     2581 cctgattccg tttgtgttct tgatgatgct ctggctcgcc agattgccag tgccttgcag
     2641 cttcgcatca ctgggacact cggaatcttg cttgatgcaa agcgaactgc gttaatccca
     2701 gtaattcgtc cccaactcga tcaactccat aacctcggct ttaggctggc ggctcatacg
     2761 cgtgctgcgg tattaaaaat ggccggtgaa tccccgaatt gatgctatcg aatgcaccgc
     2821 tctccgcacg aaaagaaatc ggcaatttcc aaatcccgct cctatgcggg aatgggtaaa
     2881 ttctgcgacc agcacgccct gaccgaattt tagcataaga ccagaagaac gaaatttacc
     2941 aagtcgaaac gaatgaaaag gtatcgagcc cgccaggaat ccatatgagc cgtcacgtca
     3001 atgcaatcgc aggacgtctg ctggcagaga cgattgcagg tctatttgga cttgagggta
     3061 cgatacggcc acgaggccga cccgctccac gttgaagcca gttccaaacg ttccagccgt
     3121 tcaattgctt cgctccgttc aaaacgttca gagccagaac tcggaagcga agaagcgctt
     3181 tggactttgt gtcctcgatt acatcgtgac atccaatcac attcatcttc tggtcactag
     3241 gcgaaatgag gaagtagata aaaagtagcc tgtccccacc tccagtttga cgatttcctc
     3301 agaaaagcat tttcttttgg ttgatcggtt gttagagttc tcggctatgg ataacgccaa
     3361 cttcgagcgc ttaattttgc ggaaacggag gcggccaaat gcgtaatggt aaaatccgaa
     3421 tcggcattat gggattgggc caaattgggc ggcacctgta tcaccttgcg cgggaaatag
     3481 atgatatcga aatcgtcgcg gtggccgata tcggcaaacc ggaaatcatt cagtacctgc
     3541 tcaatagcga cggcgtggac gaggcgccct gcgagctcca ggacaattac ctgatcaacg
     3601 agaagttccg cacaagaatg ctccagcttg cccggccgga cgaggttcca tgggatgttc
     3661 tgaacgtcca ctgtgtcgtt gacgccacgg gcagatatcg gaatcgtcgg gacatggagg
     3721 cccatctgcg cgccggtgcc ccgcgggtca ttctggcgtc gctgccggcg gaatcaatcg
     3781 accgtatcgt cgtaccggga atcaatgaag ggagcgccgc cgccgcggac aaaatgattt
     3841 cggccggttc cgcgacgacc aatgcatttg cgcttttatt gcaatctctg gatcaggcct
     3901 tgggagtcga ctgcgcatcg atgacgacgg tccacgccta tagtagcgat cagtcgctgc
     3961 agaattatgc acacaaagat ttccggcgca gccggtccgc ggcggagaat atcatcccca
     4021 acaacaatga gtccgcgcga tgggtggagc ttgttctgcc caagtttgag ggaaagttga
     4081 gcggttacgc ccttaacgtg ccggtccagc gcggatcatt gctcgatgtt aactgcgtca
     4141 tgcaggctgg caccgtcacg gccgagcaag tgaacggagc tatgcgtgcc gccgctgccg
     4201 atcatccgac gttgattggc gtgaccgatg atccgattgt ttcttcggat gtcatcggtt
     4261 gccggcaatc cttgctcttc gattcgctcg ccaccattaa agcaggaaaa agaatcatca
     4321 aagtgctggc ctggtatgag agcctggggc atgcgtgccg cgtgcttgaa gtggtccggc
     4381 actatggcag gctcgatgcc atcggaggtg ggcgatgaga gttgccatta atggattcgg
     4441 tcgaatcggc cgttctgtgt tccgtattct cgataagcgg gcggacgccg aactggttgc
     4501 gatcaacgac cttttcgacc acgacgcgct gcgatacttg ctcaactatg acaccgtgat
     4561 gggtcgtttt gatggcgccc tgcggctcga aggcaatgaa ctggtgacat cgcacggtcg
     4621 cacccgcatg cttcgtgaaa aggagccgct caaactaccc tggcgcgagc tcgacatcga
     4681 cgtcgtcatc gaatcgacgg gaatattcac taccagggaa caacttgcca agcatctgga
     4741 tgcgggagcc aagcgagtgg tgctgactgt tcccgcgaag gatgagatcg attataccgt
     4801 cgtaatcggc gtcaacgatg acgggctcaa gcccgagcac aggatgattt ctaacgcgag
     4861 ctgcaccacc aattgtcttg cgccgctggc caaggtgctc cacgattctt tcggtatcgt
     4921 ggaaggaatc atcaataccg tgcacgccta caccaacgat cagcgcctcg ccgacgtgcc
     4981 gcactcggat tggcggcgga gccgcgccgc ggcggaaaat atcataccga catccaccgg
     5041 agcggccaag gcggtgggcg aggtcctgcc tgaattgaaa ggacggctgg acggaatcgc
     5101 cgcgcgggta ccggtgcccg atggctccgt ggtcgacttg ttcgtcgagc tccagaaaag
     5161 cgttagtgcc gaggacgttc atggcgcgat aaaatccgcg gcggagtcga aacggatgaa
     5221 aggcattttg caactcatgg caacacccgt cgtatcatcc gacgtcatag ggaattccca
     5281 ctcctctatt ttcgacccgg catttaccag cgttaccggc ggtagatatc taaagtgtct
     5341 cagttggtac gacaatgaat ggggatactc caatcgcgtc tgcgatttgc tgggcttgat
     5401 cgcacggtgg gagaaacggg aataagcccg ttaagatgcc cgatggcccg tccgaaggaa
     5461 aacaaagccg cgcgcgcggt tccatcactt tcactgggat taaaggggac aggctacttt
     5521 cagcgtttgt gatcacgttc gtctatagga taagccctgc catgccgaga atcccgcggg
     5581 cgcaaccggc tggttttgtt tatcatgtga tcaaccgagg gaaacggtcg cgcaactatc
     5641 gttaataaag ctcaagatta ccaaaccttt ctttccatcg tcgcgctagc aaaagcgcgg
     5701 cacccagtta agatgtttgc tttctctctc atgccgaatc actttcactt tctgattgaa
     5761 gcttcgcatc accagtctct gagccgattc atagaagcgg cgcgagagat gtaacgtttc
     5821 tcgggggagg tccgcaaaat gacgatccgg atcagccttg tattcagtgc gattgttttc
     5881 actctctcgg ctggggcgcc ggcaaacggc gccgaaaagc ttcgcgtagc atatcccacc
     5941 ctcggtccgg gctcgacgcc ctcgtgggtg acgcatgacg cgggcttctg gaataagagc
     6001 ggactcgacg tcgaactgat tttgctgagc ggcggagcgc ggatgctgcc cgccttggtc
     6061 agcggcagtg tggagatcat tctcggctcc gatactggcg tcaccctggc aaatcttcag
     6121 ggcgccaatc tcgtgcgcat cggcgtgacg atgaattcgc tgggctactc gctggtcacc
     6181 cagccgggga ttcgctcgat ccaggatcta aaaggaaaag tgctcggcac gggccggggt
     6241 cgggacgcga gctacgcccg actggtgaag attctcgccg acaacggcct caatcccgcg
     6301 agcgacgtaa aattcctccc attgggcgag acccccaccg ggcgcctgca ggcgatcaag
     6361 agcggcctga ttcacggcgc ggttttcacg ccgcccctcg atctcgtggg cagccgtgat
     6421 ggactccgaa ttttgcacaa gatcgacgtg cccactctgg gcggcggcat caacacgacc
     6481 gcgccgatgg tgcgccaaaa ccgcggggcg ttgctcacct ttctgcgagc ctacatggaa
     6541 gggatccgct atatggccgc gcataagaca gagagtctga aagtgttctt caagtacttc
     6601 agaaatccgg acatggaggc gctagcgtat ctctacgaag ataccgtgcc gcgcttggca
     6661 aaaaacctcc ggcccaaccc ggagtcggtg agatccattc tcgaccagat cgccatcgac
     6721 gagccgcgtg cgcggcagtt gagcgagaaa gaccactggg acctgactct gctggaccag
     6781 atacagcgct agcagtccgc gtgggattta aaagccgttc aggatttggc cggacccgag
     6841 taattgggcg caactagctc gcctacatga taaagccctg cgatgccaag aatcccgcgt
     6901 gcgcaatggc tggttttgtt tatcatgtga tcaactgagg aaacggtcgc gcaactatct
     6961 ttcataaagc tcaagattac aagcctttct tcccatcctc gcgctggcaa aagcgcggca
     7021 ccagttgaga tgtttgattt cgctgatgcc gaatcacttt cactttctga ttgaagcttc
     7081 gcatcaccag tcacagcgaa atgaggaagt agatagaaag tagcctatcc gtttgcatca
     7141 cgcttgcgaa ttttcaatcg cgtctgatat gacagacaga caaacatagc ggaatgggag
     7201 ggggccaact tgggcggtta ggttgaactc tctgccactc gaaggcacta ccccctactg
     7261 cgcattcgaa gcaatacaaa gtcgatctga aggagttttt gaaaccatgc atgcctcact
     7321 ccgccgatcg aaatctacga tgcaaagatt tgcgaaattc ttcaactggc tgggagctct
     7381 tctgtctgtc tttgtgtccc ttttgctttc atcggtttca agccacagtg cagttgcact
     7441 ctcccaagaa gagctgatca atgaggcaaa aaaagaaggg agactggttt tctacgcctc
     7501 agggactact gagcaaacga cgatgtttct cgacgcattt cggtcaaagt atcctttcat
     7561 aaagacggag tattttcgca ccggaaaaac tgcgcttctt aaccgcgtcc tcaaagaaaa
     7621 tcaagcaaaa aagattatcg ctgatgcaat tcaaagcagt gtcattgaac taaatctagc
     7681 aaaaaggggc ggtgccttgg gtaaatacat tccgcacgaa gccaaggact acccggccag
     7741 ttataaggat cccgaaggtt tttggacgac gattttcatt agcagtaact tact
//
LOCUS       DASUVS010000355         6848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1681045, whole genome shotgun sequence.
ACCESSION   DASUVS010000355 DASUVS010000000
VERSION     DASUVS010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6848
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681045"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..522
                     /locus_tag="VF182_18910"
     CDS             85..522
                     /locus_tag="VF182_18910"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX6439199.1"
                     /translation="MAEYTKPLPVPDVDSQPFWDGCKAHELSAQRCRDCGTFRWPPQA
                     FCPHCYSWNFDWTKLGETGVVASFVIVHYVSIPAFQEDVPYVVAHITLDGTDGQITMI
                     SNVIGCPAQDVRVGMPVRVVFEDVTADVTLPKFRPVEKSGVLK"
     gene            617..1780
                     /locus_tag="VF182_18915"
     CDS             617..1780
                     /locus_tag="VF182_18915"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6439200.1"
                     /translation="MSGALQDLRVIDFTHALAGPFCVMLLGHLGADIIKVEPPNGDEF
                     RRLWMPPGATVDAYESLSINVNKKSIVLNLKTPKGVELARRLIERADVLVENYQKGVM
                     ERFGLDYDSLKATNPRLIYACSRGYGEWGPYANYGNTAASNNSIAGWLHSAWKNNGAT
                     GTKTLGIGDEAAGVSIALGILAALHARERTGEGQKVEVSMQEAVLGFMISSLHEHFTG
                     VSVGNRPMKVADGYFTLRVPEMSDTVWSAVARLMDRNDLVQDPRFATTVARRQNRTEL
                     EGLVRAWASGKTRREIWDGLRELDYFGAPVLSVGEVMEDRHIKERRAFIERAHPTAGP
                     TTLLAPWIHLSKTPTAIHHDAPAIGQHTDEVIGGELGLSSQELADLRAEGVVK"
     gene            1817..2869
                     /locus_tag="VF182_18920"
     CDS             1817..2869
                     /locus_tag="VF182_18920"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6439201.1"
                     /translation="MSAGLNNLAALVLLAGLLGSASAQSAKPTNLADLAKYTGPDRER
                     LLYEGAKKEGKFLWYTSLIANKEIVKIFEAKYPGVTVETYRASGTQLASRVLAEARSK
                     RPLGDVIETSPPGLMVLRDSQLLAPYTSPYLSEYPEVAKQKAPGGLVFWTTDRESYIG
                     VGYNRTIMPTADIPKNFDDLLKPALRSKMAISNDDVAARLIGGALKAKGDGFVKKLKE
                     QDVTVHGVSGPGFNEMIVSGEVALSFTAIQSNIGHAVDKGAAVAWVPMELVPAIGGGV
                     AVFAHAQRPHGALLLVDFFLSPEGQKVLVDMFRHGSPVRETGFKRWYPEEGFTTAQYD
                     EAMNRWQRLVSEFVRR"
     gene            3381..4232
                     /locus_tag="VF182_18925"
     CDS             3381..4232
                     /locus_tag="VF182_18925"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX6439202.1"
                     /translation="MPLKTQPLSDNFAVAITGVDLSKELDQNLFDEIVDVFVKKQVLV
                     FRDQHIEPQHQVSFSRRFGPFEMLYDEDQRLPGFPEIAILSNEKVDGKFIGVVAAGDF
                     WHSDQSYRREPSLATFLYARKLPKHGGDTEFADMHGAYESLPDDIKKRLEGRTGIHQR
                     SKLGNTRVAVTREGGEEYYQRRAMKNVLHPIVRTHPVSGRKGLYCSPRFTVGIEGMDD
                     AEAQPLLDTLFAYQTAPGNTYRHKWTLGDFVMWDNRSVNHQACGGYGMDDIRLLHRTS
                     TQGDEPF"
     gene            complement(4437..5465)
                     /locus_tag="VF182_18930"
     CDS             complement(4437..5465)
                     /locus_tag="VF182_18930"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6439203.1"
                     /translation="MKNFEANDAIRITRRSLLSGALATAALTFSSAALGQQMQTHLPP
                     RRTAKPKGPLVFLDYDQEEIDVAYDQAPWAPNQPEITKRNAQKSAAALARLGQPRRVA
                     YGSAEMEKVDIYVTKQAEAPINMFIHGGAWRSGNAKGAAYMAETFVDAGAHFISVDFN
                     NAPEVDGNLMVMADQVRRAVAWVHKNAASFGGDPNRLYISGTSSGGHLAAVALTTDWQ
                     KDFGLPADVLKGGLCCSGLYDLYPVSLSARAKYVKFTPEIIEKLSPQRHLEKLLVPII
                     VAHGTLETPEFQRQNREFAAAVKKAGKPIKFLVGEGYNHFEMFETLGNPYGLLGRAML
                     EQMKLRTV"
     gene            5837..6708
                     /locus_tag="VF182_18935"
                     /pseudo
     CDS             5837..6708
                     /locus_tag="VF182_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468173.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
BASE COUNT         1504 a         1902 c         2003 g         1439 t
ORIGIN      
        1 ctgccggcag gtgcgcaatc cagaaatcgc gctcgtgtgc ggcgaatgcg gcgaaagcgc
       61 catgctattg aggagggcct agcgatggca gagtacacta agccgctccc agtgccagat
      121 gtcgattcgc agccgttctg ggatggctgc aaagcgcacg agctgtcggc gcagcgctgc
      181 cgcgactgcg gaacgtttcg ctggccgcct caagcgttct gcccgcattg ctattcctgg
      241 aacttcgact ggacgaaact cggcgaaacc ggagtggtgg cttcattcgt gatcgtgcat
      301 tacgtgtcca ttcccgcatt ccaagaagat gttccgtacg tcgttgctca tattacgctg
      361 gacggcaccg acgggcagat cacgatgatc agcaatgtaa tcggatgccc agcgcaggac
      421 gtgcgcgtcg gcatgccggt gcgcgtggtt ttcgaagatg tcaccgcgga cgtaacactg
      481 ccgaagtttc ggccagtaga gaagagtgga gtgctgaaat aatggagtgt tggagagctg
      541 cgacccatcg cgccctaatt caccactcca atacgccatc actccaaaca ctccaagcat
      601 tcaggaggaa ttcaccatgt ccggcgctct tcaagaccta cgcgtgatcg actttaccca
      661 cgcgctcgcc ggtccgttct gcgtgatgct gcttggtcat ctcggggccg acatcataaa
      721 ggtggagccg cccaacggcg atgagttccg ccgcttgtgg atgccgcccg gcgccaccgt
      781 cgatgcgtac gagtctttgt cgatcaatgt caataagaag agcatcgtgc tcaacctaaa
      841 aactccaaaa ggcgtggagc tggcgcgacg gctcatcgaa cgcgccgacg tgctcgtcga
      901 gaactatcaa aaaggggtga tggagcgctt cggtttggat tacgattcgt tgaaagcaac
      961 aaacccgcgg ctgatctacg cgtgttcgcg cggttacggc gaatgggggc catacgcgaa
     1021 ttatggcaac accgcagctt ccaacaacag tatcgccggg tggcttcaca gcgcgtggaa
     1081 gaacaacggc gcaacgggaa ccaagaccct tggtattggc gacgaagccg ccggtgtgag
     1141 cattgcgctc ggtatcctcg ccgcgctgca cgcgcgcgag cgcaccggag agggacaaaa
     1201 ggtagaagtc tcgatgcaag aagcggtgct cggattcatg atcagctcgc tgcacgaaca
     1261 tttcaccggc gtcagtgtcg gcaaccggcc catgaaggtg gccgacggct atttcacctt
     1321 acgtgtgccg gaaatgagcg ataccgtttg gtcggcagta gcacggttaa tggatcgcaa
     1381 cgatcttgtt caggatccgc gctttgccac aacggtagca cggcggcaga atcgcactga
     1441 acttgaaggg ctggtcagag cctgggcgag cggtaaaacc cgtcgggaaa tctgggacgg
     1501 gctgcgcgag ctggattatt tcggtgcgcc cgtgctttcg gtcggcgaag tgatggagga
     1561 ccggcatatc aaggagcggc gtgcgttcat cgagcgcgct cacccaaccg cgggcccaac
     1621 aactttgttg gccccgtgga ttcatctctc gaagacgcca acggccatcc accacgatgc
     1681 tcccgccatc ggccagcaca ccgatgaagt aatcggcgga gaacttggtt tatcgagcca
     1741 ggagctggcg gatcttcgtg cggagggagt ggtcaagtaa ttgaagttca agaaagcgcc
     1801 cataaggagg atgttgatgt cggctggact aaacaatctt gcggctctgg ttttacttgc
     1861 cgggctactg ggaagcgcgt cggctcagag cgcgaaaccg acgaacctgg ccgatttggc
     1921 caaatacact gggccggacc gtgagcgttt gctgtatgaa ggcgccaaaa aggaaggcaa
     1981 gtttctctgg tacacatcgc tgatcgccaa caaggagatc gtcaaaatct tcgaggccaa
     2041 gtatcccggc gttaccgtgg agacttatcg tgcgagcggc acacagctcg cttccagagt
     2101 gttggccgag gcgcgctcca aacgtccgct cggagatgtc attgaaactt caccacccgg
     2161 tttgatggtg ctgcgcgaca gccagctcct agcgccgtac acctcgccct atctatcgga
     2221 gtatcccgag gtcgccaagc aaaaagcgcc cggcggtctc gtcttctgga ccaccgaccg
     2281 cgaatcctat atcggcgttg gctacaacag aacaatcatg cccacagccg atatcccgaa
     2341 aaacttcgac gacctgctca aacccgcgtt gagaagcaag atggccatta gcaatgacga
     2401 cgtcgcggcc cgattgatcg gcggtgcgct caaggcaaag ggcgacggct ttgtgaagaa
     2461 gttgaaggaa caggatgtga ccgttcacgg cgtcagcggt ccggggttca acgagatgat
     2521 tgtttcggga gaggtggcgc tgtcgttcac ggctattcag agcaatatcg gtcatgccgt
     2581 cgacaaaggc gcggcggtcg cgtgggttcc catggagctg gtgccggcca tcggcggcgg
     2641 cgtggctgtt ttcgcacacg cgcaacggcc ccacggagcg ctgctgttgg tcgatttctt
     2701 tctcagtccg gaaggacaga aagtgctcgt ggatatgttc cgccacggta gtccggtgcg
     2761 tgagaccggc ttcaaacgct ggtacccgga agagggtttc accactgcgc aatacgacga
     2821 ggcgatgaat cgctggcaga gactggtctc ggaatttgtg cgccgatgac cagaactgcc
     2881 gaaagtacaa cggtccgata aaaacacggc agaacaatgc gaagcggtgc catagatctc
     2941 catcatccta cgtccccgct gaagtggctg gagagaccaa gggcgacagc aaggctctcg
     3001 gcgtcgaggt cacccacttg ggagatggaa ccgtgcaggt ttcctcaacg gcggcccgag
     3061 gtatcgctgc cgcagctgct gcaaagtttg accgcagttg agccgtagga tttacggact
     3121 cacccagtag tcggctggtc aagtaggcaa agatttccct gcgagaagaa ctttttttat
     3181 ttgacattcc cgtgaagcgc ggttttgtta gcagacgctc gctggatctt gtcaaaattg
     3241 aagatcaggc cccgcaatca cgcattcgct tttcgcatct cttgaggcgc atatcaccgc
     3301 gggcctaact aacccgctcg atcacggctt gaccgcttta tcctatttgt gatcgacatg
     3361 gacgatggag ggccaaatcg atgccgttga aaacacaacc cttgtcggac aacttcgctg
     3421 tcgccattac cggcgtggac ttgtcaaaag agctcgacca aaacctcttt gatgagattg
     3481 tcgatgtctt cgtgaagaag caagtcctgg ttttccgcga tcaacatatc gagcctcaac
     3541 atcaagtttc cttcagccgg aggtttggcc cgttcgaaat gttgtatgac gaagatcagc
     3601 ggctgccggg ctttccggaa atcgccatcc tttccaatga gaaggtcgac ggtaaattca
     3661 tcggcgtcgt cgcggccggt gatttctggc attcggacca gtcttacaga cgggagcctt
     3721 cgctcgcgac ttttctctat gcccgcaaac tacccaagca cggcggcgat acggaattcg
     3781 ccgatatgca cggcgcctat gaaagcctgc ccgacgacat caagaagcgc ctcgaggggc
     3841 ggacgggtat ccaccagcgc agcaagctgg gcaatacacg ggtggcggtg acccgggagg
     3901 gcggcgagga atattaccaa aggcgggcga tgaagaatgt gctccatccg atcgtccgca
     3961 cccatccggt atcggggcgc aagggtcttt actgctcgcc gcgttttacg gttggcatag
     4021 agggcatgga cgacgccgag gcgcagccgc tgctcgatac gttattcgct taccagacgg
     4081 cgcctgggaa cacgtatcgt cacaaatgga ccctgggcga tttcgtgatg tgggataatc
     4141 gcagcgtcaa tcatcaagcc tgcggtggtt atgggatgga tgacattcgc ttgcttcatc
     4201 gcacatcgac acaaggagac gagccattct aagtggaagg aatagccgtt taaatcgttc
     4261 aagccgttca accgctgcgc tcggttcaaa tcgttagaac gagtcggttc tgaacgttcc
     4321 gcgactccag gtgttccaaa cgttcaatcg caacgcggac gggtaaagct tgacggtctg
     4381 acttgagtgg ttgccttatg ctataccggc ggcagcacag atcatggcgc caccgtttac
     4441 actgtcctca atttcatctg ctcgagcatc gctcgaccga gcaaaccgta gggattaccg
     4501 agtgtctcga acatctcgaa atggttgtag ccttcgccta caagaaactt tatcggcttt
     4561 cccgcttttt tcaccgccgc ggcgaactcg cggttctggc gctggaactc gggggtttcg
     4621 agcgtgccgt gagcgacgat gatcgggacg agcagtttct cgagatgacg ttgtggtgag
     4681 agtttctcga taatctccgg cgtgaacttc acgtattttg cgcgcgccga gagactcacc
     4741 ggatagagat catacaagcc gctgcagcag agccctccct tgagaacatc cgccggcaat
     4801 ccgaaatcct tctgccagtc ggttgtgaga gcaaccgccg caagatgccc gccggacgac
     4861 gtccccgata tgtaaaggcg attagggtcg ccgccgaagc tcgcggcatt tttgtgaacc
     4921 caagcaacgg cgcgccgcac ttgatcagcc atgaccatga gattgccgtc cacctcgggc
     4981 gcattgttga agtcgacgga aatgaaatgt gcgcccgcat cgacaaaggt ctccgccata
     5041 taagccgcgc ccttcgcatt cccgctccgc catgcaccgc catgaataaa catattgatc
     5101 ggcgcttcgg cctgctttgt tacataaatg tcgactttct ccatctcggc cgagccatat
     5161 gcaacacggc gcggctggcc gagacgagct aaagcggcgg cgcttttctg cgcattgcgc
     5221 ttggtgattt ctggttgatt gggcgcccac ggcgcctggt cataagccac gtcgatctct
     5281 tcctggtcgt aatcgagaaa gacgagcggc cccttcggct tggccgtcct tcgcggcggc
     5341 agatgggtct gcatctgctg ccctagagcg gcgctggaaa acgttagagc tgctgtagcc
     5401 agcgcgccgg agagcagcga gcgccgtgtg attcggatcg catcgttcgc ttcgaagttt
     5461 ttcatgatcg gcctcctttt gactgaccag taaacccgtt gcgttgatgg ttctgtggga
     5521 agttctgaat ataaacgaaa ggctcgcaga aacacaaccg agaaaagtgt ttgtccaaag
     5581 gatggtcctc tgtttcttat ccgagcgatc tttagtaagg atgcaaagaa tccaataaca
     5641 actgccaaac agatcggagt gacggtaccg ccgaagtatt aagcagagcg gactgcgtca
     5701 tccgatgagt tattttccat tttcgattgg ccatctcgaa ctgtttctgc cgagacaaaa
     5761 caatctgcag gggagatttt ttatgggagc aatcatggcg tctaggttac ttttcctgtt
     5821 tcttgtcttg ggtccgatgg tcttcatgac cggttgtgag cgtgcagcgt cgtcggccca
     5881 aaaaagcgag tcgcagggtt cagcccggtc ggacctttgg cgtgtctacg atcggtccct
     5941 caaggaagcc aagtacatcg atctgacaca caccctgacg ccatcaatcc cggtatggaa
     6001 aggctttgcg caggcaaaat tcgctccgac gatcaatcct gaaaccaaac acccttacag
     6061 ttacgaaaag gatggcttcg aggcgacgca ctacgatttg cccaccgacc aattcggcac
     6121 ccagctcgat ccgccggcgc actgggctcc ggagtttccc gccattgacg agctgccggc
     6181 gacctatgcc gtgcgcccgc tggcggtaat ttccatcgtc gatcaggtgg ccaaagagcc
     6241 tggctatcat ctccagcgct cggatatcga gaggtgggag agacgcaacg gcaagattcc
     6301 ggcgggttcg gtggtgatgg tgcgctcgga ttggtcgaag cgctggcccg atccggcgcc
     6361 ggcaagcgag acggtttttc ccggcgtctc gttggaggcg ctgcaatttc tccacgagga
     6421 acgtcacatc ctatttcacg gccacgagcc gctcgacacc gacaccactc cgaccctcga
     6481 agaggcttgg ctgatgcata acggctatgc ccaggcggaa ggggtggcga acttggacaa
     6541 ggtcgccgag aagggttgtt taattacgat cgggtatccg aaattcgccg gaggcctagg
     6601 cggctacgcg cgctacatcg ccatctgccc ggcggactgg aaatacggcg tatcggtcgg
     6661 cgaagttgcc gaagcgccgc ttccaggagc gacaagattc ttcagtgaga cgacgagctc
     6721 ggcatgcgcg tgcgcaagta acgaaagcga cgcgtatttc gtcccaggca gtccacatcg
     6781 agcggggtca cgcattagcc ggcgggtcaa gtgggcacag attttcctgc ggagaagaat
     6841 ttcttttt
//
LOCUS       DASUVS010000356        12018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_587682, whole genome shotgun sequence.
ACCESSION   DASUVS010000356 DASUVS010000000
VERSION     DASUVS010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12018
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587682"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="VF182_18940"
     CDS             <1..1138
                     /locus_tag="VF182_18940"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEX6439204.1"
                     /translation="FGNASSRNHVFGWESEAAVDHAREQVASLIHAASPREIVFTSGA
                     TESDNIAIKGIAETYARQGNHIVTCAIEHKAVLDSCKALERKGYRVTYLPVWANGLLD
                     LNRLRDALTDKTILISIMAANNEIGTIEPVREIGKIAKEKGILFHTDATQAVGKIPVN
                     VEDMGIDLLSLTAHKIYGPKGVGALYVRSSKPRVKLNPLIEGGGQERGLRSGTLNVPG
                     IIGLGKACEIAQQDLMADGERLTGLSERLKAGIIGQLDEVSVNGDPLQRLPGNLHLSF
                     AYIEGESLLMGLKDIAVSTGSACTSASLEPSHVLRAIGLEEHLAHSSIRFGLGRFNTE
                     EEVDYTVRRIVEEVRRLRQLSPILRARKAKLQSHANHKSQESAS"
     gene            1167..1496
                     /locus_tag="VF182_18945"
     CDS             1167..1496
                     /locus_tag="VF182_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX6439205.1"
                     /translation="MAAGISVTDRAAARIRELVTAENREGQGLRVKVVGGGCSGLQYK
                     VDFDSPKGSDKVFEKDGARVLVDMKSLLYLTGTELDYKEELMQSGFVFQNPNVKKACG
                     CGASFTV"
     gene            1530..2990
                     /gene="sufB"
                     /locus_tag="VF182_18950"
     CDS             1530..2990
                     /gene="sufB"
                     /locus_tag="VF182_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX6439206.1"
                     /translation="MKSANNTLEELAGREYRYGFITDIESDTVPPGLSEDIIRLISAK
                     KNEPAFMLEWRLKAYRHWAKLEKGQAEPKWAMVHYPAIDYQSISYYSAPKNKGDAPKS
                     LAEVDPELLNTYEKLGIPLREQERLAGVAVDAVFDSVSVATTFKGKLAEMGVIFCAFS
                     EALQNHPDLIQKYLGSVVPYTDNFFASLNSAVFTDGSFCFIPKGVRCPMELSTYFRIN
                     AAETGQFERTLIIAEERASVSYLEGCTAPMRDKNQLHAAVVELIAHDDAQIKYSTVQN
                     WYPGDAQGRGGIYNFVTKRGKCLGKRSKISWTQVETGSAITWKYPSCLLQGDDSVGEF
                     YSVALTNNYQQADTGTKMIHLGKNTKSTIVSKGISAGHGQNTYRGLVKIMKGATNARN
                     YSQCDSLLLGDKCGAHTFPYLEVLNSTAQVEHEASTSKIGEDQLFYCRQRGISTEDAV
                     NLIVSGFCKQVFRELPMEFAVEAQKLLGVSLEGSVG"
     gene            3012..4241
                     /gene="nifS"
                     /locus_tag="VF182_18955"
     CDS             3012..4241
                     /gene="nifS"
                     /locus_tag="VF182_18955"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HEX6439207.1"
                     /translation="MTREVYFDNNATTRVMPEVFEAMAPFYADFYGNPSSIHRFGSQV
                     GERLSQARGQVASLIGAADPVEVIFTSCGTEGDNAAIRGLLEMRPEKRHIITTQVEHP
                     AILGLCQHLERKGYRVTWLGVDQNGMLDLDELCAAITNETALVSIMFANNETGVIFPI
                     EQIGEIVKSKGVPFHVDAVQAAGKLTIDIKKSPVDLMTISAHKFHGPKGVGALYVRRG
                     ITFSPFIIGGHQERGRRAGTENVAGIVGMGKAAELAVKNHQDDSVRIRALRDQLQNSL
                     LQSCPSARVNGDKLQRLPNTLNISFRYLEGESILVLLDQHGICASTGSACTAGSSEPS
                     HVLRAMKTPADWLQGAVRFSLSRFNTEDEVRFINEKMPSIVKRLEGLSALGKLAVQER
                     NRTPGSGNPSGVGTARG"
     gene            4254..4700
                     /locus_tag="VF182_18960"
     CDS             4254..4700
                     /locus_tag="VF182_18960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX6439208.1"
                     /translation="MQIPRRVDYGLRAIIYLSTQDPEKCCSIAEIARQQNVPKKFLEK
                     IIQDLMRRGLIRSKRGSCGGYTLARMPDQISFYDVIEALEGPIAVNVCMNEDLSCDQL
                     PRCAMVGVWSEIQQKVTEVFTRTTLADLRRTPCRDNLSASSLSSAA"
     gene            4758..5216
                     /locus_tag="VF182_18965"
     CDS             4758..5216
                     /locus_tag="VF182_18965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439209.1"
                     /translation="MSYDTGLSMEERVTSLFQPDTLMPEQYLETFRRKLYLEPEKKLL
                     LAVLEDAIACFQKYLFAKDGKGRALFLEAEEWILDEDTDWLFSFANLCEMLGFEPSYL
                     RDGLMRWKAEKLESHSKAKVYQIAVRTTRQKREVSASRRSTQRMRKAASR"
     gene            5335..6339
                     /locus_tag="VF182_18970"
     CDS             5335..6339
                     /locus_tag="VF182_18970"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6439210.1"
                     /translation="MERRRSKKSSAFSRFFYRLRYGLGAYVLRIFISILGRLPDRLVV
                     SASTFWAWITHKLLWKYRIRMEKNISVAAGDRFPDKVERKAMVWRIWKSFTRGLFEAG
                     ALIHFPKEAILAKIRVEGEQHLRDALARRKGVLALSAHLGNFALIGARLAAAGYPFSV
                     VVKHPSNDEFARLITQYRAQVGIDTIAAKPRREAVRAIVRALRENRIVLVVADEFKSG
                     EITVEFMGQALPAPRGPATLALRTGAVTLPMFATRQRDDSIVLEIGRPIEAVHRESLE
                     ETITATTALYTRHLEDAIYRYPDQWSWLGFPRKGRIVRAEMFNSRPSRTEANATHRAS
                     "
     gene            complement(6363..6965)
                     /locus_tag="VF182_18975"
     CDS             complement(6363..6965)
                     /locus_tag="VF182_18975"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6439211.1"
                     /translation="MMQRYDYLKAIAGDTGDALAVCTGWGAREWWALRPSDGNLKTRT
                     LGLVSSIAAGLALALPHRKVIAIDGDGAFLMNLCGLPTIALQNPPNLIHLLFDNGVYE
                     ASGGTATASHVADAVSLAKGAGYPHAFWVQTPEEFRARFLDARHSNRLTLIAVKVEPG
                     QPKTLPSLKLDELENKYLFMRYLEQTENRSILSPGFDSKV"
     gene            complement(6962..7474)
                     /locus_tag="VF182_18980"
     CDS             complement(6962..7474)
                     /locus_tag="VF182_18980"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6439212.1"
                     /translation="MEEMDPRRSALAIVSGVKKAGIDFVATLPDEKMLEVIRAVESDS
                     ELRHIPLCREEEGIGICAGAYLAGRKTALIMQNAGFLNSCNALTTTCLQFQIPIVLLI
                     YYAGDIGDRGFTTLGAVTEPVLQALGIRSYILRRNDEIGETIAGAEVLAEDSKRPVAV
                     LLTKSVLGVK"
     gene            7962..8573
                     /locus_tag="VF182_18985"
     CDS             7962..8573
                     /locus_tag="VF182_18985"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX6439213.1"
                     /translation="MVERDISRRGIKDQELLRVMATLPRHVFVPEEVRSSAYQDRPLP
                     IGSGQTISQPYIVAFMTQLLELKGRERVLEIGTGSGYQTAVLAALAEQVFSVEILAEL
                     SAAAERTLKQLGIQNVKLKVGNGYHGWSKEAPFDAIMITAAGPKIPPPLWNQLREGGV
                     LIMPLGKEGHTQQLIRARKIGGRQVIQEHSAVIFVPLTGGTQQ"
     gene            complement(8536..8907)
                     /locus_tag="VF182_18990"
     CDS             complement(8536..8907)
                     /locus_tag="VF182_18990"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX6439214.1"
                     /translation="MRLPFHISQRYVLYLLGGLILLLAVVTVVGERGAIHLWRLRGEK
                     SRIDEQNYRLQKENEVLRQRVSRVRNDNHYLEKLAREELNLVRPGEVIYRFPKSNPDN
                     DRPDLGNEAPTAASRPSTARK"
     gene            8979..10265
                     /gene="eno"
                     /locus_tag="VF182_18995"
     CDS             8979..10265
                     /gene="eno"
                     /locus_tag="VF182_18995"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX6439215.1"
                     /translation="MKIQRVHAREVLDSRGQPTVEVEVMLSNGFTGRATVPSGASTGI
                     HEAMELRDGGKRFLGKGVAKAVLHVNQKIAPRLRGKEAKEQGAIDEIMLNLDGSANKG
                     KLGANAILGVSLAVAHAEAQAQGLSLFRYLGGSQAKTLPVPMLNVLNGGAHADNNVDL
                     QEFMIVPFGMKSFKEALRGAVEIFQTLKRILHDRSYSTSVGDEGGFAPRVKSNNEAIE
                     LLLEAIANAGYKVGSQVALALDVASSEFFRDGKYVFKKSDGRQRNREEMVRLYEDWVR
                     QYPIISIEDAFAEDDWEGWQMVTQALGKKVQLVGDDLFVTNKVRLKRGIDSEVANSIL
                     VKVNQIGSLTETLETMKLAKEAGYTTIMSHRSGETEDVTIADLAVATNAGQIKTGAPC
                     RGERTAKYNQLLRIEEELGSRAVYAGKKAFNPKRGK"
     gene            10225..11007
                     /gene="nth"
                     /locus_tag="VF182_19000"
     CDS             10225..11007
                     /gene="nth"
                     /locus_tag="VF182_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX6439216.1"
                     /translation="MPERKLSIPNAASKSPGSQSIRLIRPRRARENLKEKIARTGVIA
                     TGLRQAYPELRVPLDYGTTFQLLVAVILSAQCTDAAVNKVTPELFRRFPTPDRMASAP
                     LAEIESMIRPLGLFRAKARSLKACAQQLGERFNGQVPGTMEELTTLAGVGRKTANVIL
                     GHAFDVPGIIVDTHCKRVSRRLGLTRSEDPVQIERDLSKLLPAEEWTGFSHRLIIHGR
                     RICHARKPDCKACIVERLCPSSSLIWDRPNPRTHAHNRQVHG"
     gene            complement(11437..>12018)
                     /gene="surE"
                     /locus_tag="VF182_19005"
     CDS             complement(11437..>12018)
                     /gene="surE"
                     /locus_tag="VF182_19005"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX6439217.1"
                     /translation="PRRLAVNGTPSDCVKLALTGLLPVRPNLVVSGINKGPNLGDDII
                     YSGTVSAAIEGALLTVPSIAVSLVTFRDFDFRAAAEFTGFLVSSIQDTGGIPPETLLN
                     INVPPLPKSELKGWRLTRMGKRHYSENIVERVDPRGGKYYWIGGDDLGFDKEDGTDCV
                     AVHEGYVSVTPLQVDLTNYRVLENNTLPNFVWP"
BASE COUNT         2890 a         2988 c         3372 g         2768 t
ORIGIN      
        1 gttcggcaat gcttcgagcc gcaatcacgt ctttggttgg gagagtgaag cggcggtaga
       61 tcatgcgcgt gaacaggtgg cttctttgat ccatgccgca tctccccggg agatcgtttt
      121 taccagcggt gccaccgaat cggataatat cgcaatcaaa ggaatagccg aaacctatgc
      181 gcggcagggc aaccacatcg ttacctgcgc catcgaacac aaggctgtgc tcgacagttg
      241 caaggccttg gagagaaaag gctatcgagt aacttatctg ccggtctggg caaatggctt
      301 gctcgatcta aatagacttc gggatgctct caccgataag acgatcctga tttcgataat
      361 ggctgctaac aatgagatcg gcactatcga accggtgcgt gagattggaa agatagcgaa
      421 ggaaaaggga attttgtttc ataccgatgc gacccaggcg gtaggcaaga ttcccgtaaa
      481 tgtagaagat atgggcatcg acctgctctc gctgacggcc cacaagatct atgggcccaa
      541 gggggtagga gcgctgtacg tccgatcatc caagccgcgg gttaaactca atccgttgat
      601 cgaaggcggc gggcaggaac ggggtctccg gtcagggact ctcaatgttc cggggatcat
      661 cggtctaggc aaagcttgtg aaatagcgca acaagatttg atggccgatg gcgagcgtct
      721 gaccggattg agcgaacggt taaaggccgg gattatcggc cagctggatg aggtgagtgt
      781 caatggtgat ccgttacagc gactgccggg gaacttacat ttgagctttg cctatatcga
      841 aggcgaatcg ctcttgatgg ggcttaagga tattgctgtc tcaaccggtt ccgcctgcac
      901 ttcggcaagc cttgaacctt cccacgtctt gcgggcgatt gggttagagg agcatttagc
      961 tcattcttcg attcgttttg gcttagggcg gtttaataca gaagaagaag tcgattatac
     1021 cgtgcgccgt atagtcgagg aagtgcgccg gctgcggcag ttgtcgccaa ttcttcgcgc
     1081 cagaaaggcg aaattgcaaa gccacgcgaa tcacaaatca caggagagtg cctcctagtc
     1141 attggtttgt ataggagaat ggatgtatgg ccgcagggat tagtgtcacc gatcgtgcag
     1201 cggcacgaat tagagaatta gttactgccg aaaaccgcga agggcaaggg ctcagagtca
     1261 aagtagtcgg cggcggctgt tcgggacttc aatataaggt agattttgat agcccgaagg
     1321 gttcggacaa agtgttcgag aaagatgggg ctagggtgct ggtcgatatg aagagcctgc
     1381 tttacctcac cggcacagag ctggactata aagaagaact gatgcaatca ggatttgtct
     1441 ttcaaaatcc caacgtcaag aaggcgtgtg gttgcggcgc atcttttacc gtttgagcgt
     1501 aatggcagtg gtcatgggaa attactctta tgaaaagcgc taacaatacg ctggaggaat
     1561 tagccgggcg cgagtacagg tacgggttca tcaccgatat cgaatcggat accgttccgc
     1621 caggtttaag cgaggatatc atccggctaa tttcggcaaa aaagaacgaa cccgcgttca
     1681 tgctggagtg gcgcctcaaa gcctaccggc actgggccaa gttagagaaa ggtcaggccg
     1741 agccgaaatg ggcgatggtt cattacccgg ctatagatta tcagtccata agctactatt
     1801 cggcgccgaa aaataaaggg gatgcgccca agagtttggc agaagttgat cccgaacttt
     1861 tgaacaccta tgaaaaactt ggcattcccc tgcgagaaca ggagcgtctt gccggagtag
     1921 cagtggacgc agtgtttgac agcgtttctg tggcgacgac tttcaaagga aaattggccg
     1981 agatgggggt aatcttctgc gccttctcag aggcgctgca gaatcaccct gatttgattc
     2041 agaaatattt aggatcggtg gtgccgtata ccgataactt ttttgcttct ctgaattccg
     2101 cggtgtttac tgatggttcg ttttgtttca tccccaaagg ggtgcgttgc ccgatggagc
     2161 tgtccaccta cttccggatt aacgccgccg aaaccgggca gtttgagcgt actctgatca
     2221 tcgccgaaga acgcgcctca gttagctatc ttgaaggttg cacggcgcca atgcgcgaca
     2281 aaaatcaatt acacgcagcg gtggtcgaat taatcgcaca tgacgatgcg cagatcaagt
     2341 attcaacagt tcaaaattgg tatccggggg atgcgcaggg gcgcggcggg atttacaact
     2401 tcgtcacaaa acgtggcaaa tgcctaggaa agcgttcgaa gatttcgtgg actcaagtgg
     2461 aaacggggtc ggcgattacc tggaagtatc caagctgttt acttcagggc gatgattcgg
     2521 tgggggaatt ctattcggtt gccttgacca ataactatca gcaagcagat accggcacca
     2581 aaatgattca tctgggtaag aacaccaaga gcacaattgt atcaaaaggc atctctgcgg
     2641 gccatgggca gaatacctac cgcggcttgg ttaaaatcat gaaaggcgcc accaatgcgc
     2701 ggaactattc gcaatgcgac tcgctactgc tcggtgacaa atgcggcgcg catacctttc
     2761 cgtatcttga agttctgaat agtacggcgc aagtcgagca cgaagcatcc acttcaaaaa
     2821 tcggcgaaga tcagcttttt tactgccggc aacgtggaat ctccacggaa gatgccgtga
     2881 atttaatcgt cagtggattt tgcaagcagg tgttccgcga attgccgatg gagtttgcgg
     2941 ttgaagctca gaagctactc ggcgtcagcc tggaaggcag cgtgggttga tagctgcgtc
     3001 gtgagtaaat gatgacacgc gaagtctatt tcgacaacaa tgcgaccacg cgggtgatgc
     3061 ctgaggtctt cgaggcgatg gcgccgtttt atgccgattt ctatggcaat ccgtcgagca
     3121 ttcatcgttt cggcagccaa gttggcgagc gccttagcca ggctcgggga caagtcgcga
     3181 gtttgattgg cgccgccgat cccgttgagg tgatttttac cagctgcggc accgagggag
     3241 ataatgcggc gattcgtggc ttgctcgaga tgcggccgga gaagcgccac atcattacta
     3301 ctcaggtcga acatccggca attttgggtt tgtgccagca cctggagagg aagggctatc
     3361 gagtcacctg gcttggggtg gatcagaacg ggatgctcga tttggacgag ctgtgcgccg
     3421 ccataaccaa cgagactgcg ctggtctcga ttatgtttgc caacaatgaa acgggtgtaa
     3481 tcttcccgat tgagcaaatc ggcgagatcg tcaaaagcaa gggagtcccg tttcacgttg
     3541 acgccgtgca ggcggcaggc aagctgacta tcgatataaa gaaaagtccg gtcgatctta
     3601 tgaccatatc agcgcataaa tttcatgggc ctaagggcgt cggcgccctt tacgtgcgcc
     3661 gcggcattac cttttcacct tttatcatcg gcggacatca ggagcgcggc cggcgggccg
     3721 gaacggaaaa cgtggctggt attgtcggta tgggcaaggc ggcggaattg gctgtgaaaa
     3781 atcaccagga tgattccgta aggattcgcg ctttgcgtga ccaactgcag aattccttgc
     3841 tccaatcttg tcccagcgcc cgcgtcaacg gcgacaaact gcaacgactg cccaatactt
     3901 taaatatcag tttcaggtac ttggaaggag aatccatttt agttttgctc gatcaacatg
     3961 ggatttgcgc ttcaaccggt tctgcctgca ccgccggttc ctcggagccg tcgcatgttc
     4021 tgcgcgcgat gaagacgcca gcggactggc tgcaaggcgc ggttcgattt agcttgagcc
     4081 ggttcaatac agaagatgag gttcgattta tcaacgaaaa gatgccttcc atcgtcaaaa
     4141 ggttagaggg actgtctgca ttgggaaaat tagccgttca agaacggaat cggacccccg
     4201 gatcaggcaa tcccagcggc gtcggaacag caagggggta ggcgatgagc ctgatgcaga
     4261 ttcctcgaag ggtggattac gggttgcgtg cgatcatcta tctttcaact caggatccgg
     4321 agaaatgctg ctcaattgcc gaaatagccc ggcaacagaa tgtgccaaaa aagtttttgg
     4381 agaaaatcat ccaagatctt atgcggcgcg ggttgatccg atctaaacga ggttcgtgcg
     4441 gtggttatac gctcgctcgc atgccggacc agatatcgtt ctatgacgtt atcgaggcgc
     4501 ttgaagggcc gatcgcggtc aatgtatgta tgaatgagga cctcagctgc gatcagctgc
     4561 cccgctgcgc tatggtggga gtctggagtg aaattcaaca gaaggtgacg gaggtgttta
     4621 cccgaacaac tttggcagat ttacggcgca ctccgtgtcg ggataatttg agcgcctcgt
     4681 ctctctcgag cgcggcgtga acaggctcaa aaagttggct aaggaggcag gccgtaaccg
     4741 aaagggaggt gatagttatg agctacgata ccgggcttag catggaagaa agggtgacct
     4801 cgctgttcca gccagacacc ctgatgccag aacagtatct ggaaactttc cgccggaagc
     4861 tctatctcga gcccgagaaa aagctcttgc tcgcggttct tgaagacgcc atcgcttgct
     4921 ttcagaagta cttgtttgcc aaggatggta aggggagagc gctttttctc gaggccgaag
     4981 aatggatctt ggatgaagat accgattggc tattctcatt tgccaacctt tgcgagatgc
     5041 tggggttcga gcctagttat cttcgcgatg ggctgatgcg ttggaaagcc gagaagcttg
     5101 agagtcattc caaggccaaa gtttatcaaa ttgccgtaag aacaactcgc caaaaacgtg
     5161 aagtctcagc ctcacgcaga tcgactcagc ggatgcgtaa agccgcaagc cgttagctgg
     5221 ctttacctga attaagagaa agttgaaggc ggtgaatgcc ctgcggtgtt caccgccttc
     5281 tttgtgggct cattttggct tgcaaagccg gcaaaacgca ttcataaatc ttttatggag
     5341 cgtcgcagat caaaaaaaag ttcggcattc agcagatttt tctatcgcct gagatatgga
     5401 ctcggtgcgt acgttttgcg tatcttcatt tccatcctcg gtcggttgcc ggatcgtttg
     5461 gtggtgtccg cttcaacttt ttgggcttgg attacgcaca agctgctgtg gaaatatcgg
     5521 attcgcatgg agaagaatat ctccgtagcc gccggcgatc gtttccccga caaagtcgag
     5581 cggaaggcaa tggtgtggcg catttggaag agctttacgc gcgggctctt tgaggccggt
     5641 gcgttaattc atttcccaaa agaagcaatc ctcgcaaaaa tccgggttga aggggaacaa
     5701 catctgcgcg atgctctggc gcgacgaaaa ggagtgcttg ctttgagcgc gcacctcggc
     5761 aatttcgcgc tcattggcgc gcgattggcg gcagcgggct atccgtttag tgttgtagtg
     5821 aaacacccca gcaacgatga attcgcacgg ctaatcaccc aatatcgggc gcaagtagga
     5881 atcgatacga ttgcggctaa acccaggcgg gaagcggtgc gtgcgattgt ccgggcactg
     5941 cgcgagaatc ggattgtact ggtcgttgcc gatgagttta aatccggcga gatcacggtt
     6001 gaattcatgg gacaagcctt gccggcgcct cgaggcccgg cgactctggc tttgcgcacc
     6061 ggcgccgtga ctttgcctat gtttgccacc cgtcagcgcg atgactctat cgttctcgag
     6121 attggccgcc cgatcgaagc cgtgcatcga gaaagtctcg aagaaaccat tacggcaaca
     6181 acggcgcttt acacgcgcca tcttgaggat gccatttacc gctatccgga tcagtggagt
     6241 tggcttggct ttccgcgcaa gggcagaatc gtgcgcgcgg agatgttcaa ttcacggcca
     6301 tcgagaaccg aggcaaatgc tacacatcga gcatcataat acttggtccc gtgagcgaaa
     6361 ggtcatactt tcgaatcgaa tccagggctc aatattgatc tattctcggt ttgctccagg
     6421 tagcgcatga acagatactt gttctccaat tcgtcaagct ttaacgatgg taatgttttc
     6481 ggctgtccgg gctccacctt aaccgcaatc aaggtgaggc gattgctgtg ccgagcgtca
     6541 agaaatcgcg ctcgaaactc ctccggcgtc tggacccaga aggcatgcgg gtatcccgct
     6601 cccttggcca gtgacacggc atcagcaacg tgagaagcgg ttgcggtacc gcctgaggct
     6661 tcgtaaacac cgttatcaaa aagcagatgg atcagattcg gtggattctg tagagcgata
     6721 gttggcagcc cgcaaaggtt catcagaaac gcaccatccc cgtcaattgc gatcactttg
     6781 cgatgcggca acgccaaggc aaggccggct gcgattgacg agactaaccc tagcgtccgc
     6841 gttttgagat tgccgtcgct gggtcgaagc gcccaccatt cccgtgcgcc ccaccccgtg
     6901 caaaccgcca acgcatcgcc ggtatcgccg gcgatagcct ttagatagtc gtaacgttgc
     6961 atcatttcac tcctagcacg ctcttagtta agagcactgc aaccggtctt tttgaatcct
     7021 cggctaggac ctcggcgccg gcgatggttt cgccaatctc gtcgttcctg cgcagaatgt
     7081 atgaacgaat ccccaacgct tgaagcaccg gctcagtgac cgcgcccaga gtggtgaagc
     7141 cgcgatcgcc gatatcaccg gcataataaa tcaacagcac aatcggtatc tgaaactgca
     7201 aacaagtggt ggtcagcgca ttacagctgt tcaagaaacc ggcattttgc ataatcagag
     7261 cggtttttcg cccagccaga taagcgccgg cgcaaatgcc gataccctct tcctcgcgac
     7321 acagcggaat gtgccgaagc tctgagtcgc tttcgacggc ccgaatcact tcgagcatct
     7381 tttcgtccgg caaagtggcg acgaaatcga tgccggcttt cttgactccg ctgacgattg
     7441 ccagggcgga tcgtctagga tccatttctt ccatgaaacg agacctcttt tacttactaa
     7501 gtgagcgcag cgggttcgat cagttgccca tctcaatacc tgggccactg ggacgaaata
     7561 ctgcgtgtct ttaaccttgt caaggttggt tgctgagcct atgcttgaca caagggaggc
     7621 agtgcctaaa gtgaaattac ctcgtgaagc gtcagagtct ccagccagac ggtctccgtg
     7681 gctgcataaa cacgattttg tcgcggatta gcatgaggat agaggcaggc gttcgggaga
     7741 tcaatgagcg cgtcgattgg aacttctcaa tttgtctgga accatcgacg tggatgacaa
     7801 gtttatctcg tacggcgggt caaaggttta gagccatatc ttgtcttggc ttccactgct
     7861 tttgtttact actcatcgca tcctcatctt taatgttcgg atctcaggat tcagcggcgg
     7921 gcgaacatcc tgaaacgacc ttcactgaag cgcgtaggat catggtcgaa cgggacattt
     7981 cccgacgcgg catcaaagac caggagctcc ttcgggttat ggcaactctg ccccgccacg
     8041 tattcgtacc ggaagaagtg cgctcttccg cctaccaaga tcgcccgctg ccgattggct
     8101 ccggacaaac catctctcag ccgtacatcg tagccttcat gacgcagctg ctggagctta
     8161 aaggacgcga gcgagttctg gaaatcggca ccggttcagg ctatcaaacc gcggtgcttg
     8221 ccgccttagc ggaacaagtc ttctcagtag agattcttgc tgagttaagc gccgccgccg
     8281 aaaggactct taaacagctg gggattcaaa atgtaaaatt aaaagtcggc aatggttatc
     8341 acgggtggtc caaggaggcg ccgttcgatg ccatcatgat tactgcggcg ggacccaaaa
     8401 ttcctccgcc tctgtggaac cagctccgtg agggcggcgt cttaatcatg cccttaggta
     8461 aggaagggca tactcaacag ttgattcgcg ccagaaagat cggcggccga caagtgatcc
     8521 aagagcacag cgccgttatt ttcgtgccgt tgacgggcgg gacgcagcag taggagcttc
     8581 gttacctaga tcgggacgat cgttatcagg gttagactta ggaaaacgat agatgacttc
     8641 gcccggacgg accaggttga gttcctcgcg agctagtttt tccaggtagt ggttgtcgtt
     8701 gcggacgcgc gagacccgct gtcgcaaaac ttcgttctcc ttctgtaatc gataattttg
     8761 ttcgtcgatt ctgctttttt cgccgcgcaa tcgccataaa tgaatcgcgc cgcgctctcc
     8821 aacgaccgta accaccgcaa gcagtaaaat caggccgcct agcaggtaga gaacgtaacg
     8881 ttgggaaatg tgaaatggta atctcattgg cgccgttaat ggcaatataa cataaattat
     8941 gttttgctgc tgcgaagaca ttttcttgga ggttgccgat gaaaatccaa cgggttcacg
     9001 ctcgcgaagt gctcgattct agaggccagc ctaccgtgga agttgaggtg atgcttagca
     9061 acggattcac cggtcgtgcg acggttcctt ccggtgcgtc gaccggcata cacgaagcga
     9121 tggagttaag agacgggggc aagcgatttc tgggaaaagg agtggccaag gcggtgttgc
     9181 atgtgaatca aaagattgcc ccgcggctac gcggcaaaga agccaaagag cagggggcca
     9241 tcgacgaaat catgctcaac ctcgatggtt cagcgaacaa gggtaagctc ggcgccaacg
     9301 cgatcctcgg ggtttcgttg gctgtcgctc acgcggaagc ccaagcgcaa ggtctttctc
     9361 tctttcgtta tttgggcgga agtcaggcga agactctacc cgtccccatg ctaaacgtgt
     9421 tgaacggtgg tgctcacgcc gataacaatg tcgatcttca agaatttatg atcgttccat
     9481 ttggcatgaa aagcttcaag gaggcattac gaggagcggt ggaaatcttt cagacgctca
     9541 agaggatttt acatgaccgc tcgtattcca ccagcgtcgg cgacgaaggc ggttttgcgc
     9601 cgcgtgtgaa aagcaacaac gaggccatcg aactgctgct tgaagcgatt gcaaacgccg
     9661 gttacaaagt tggctcacag gttgcgctgg cactggacgt cgcttcgagt gaatttttca
     9721 gagacggcaa atatgtcttc aaaaaatccg acggacgtca acgtaatcgt gaggaaatgg
     9781 tgcggcttta tgaggattgg gttaggcagt atccgatcat ttccattgaa gatgccttcg
     9841 ctgaagacga ttgggagggc tggcagatgg tgacgcaggc tctcggtaag aaagtacaat
     9901 tggtcggcga tgatctgttt gtcaccaata aggtgcgatt aaagcgcggc atcgattccg
     9961 aagtcgctaa ttcgatcctg gtgaaagtga atcagatagg cagcctgaca gaaacgttgg
    10021 aaacgatgaa attggcgaaa gaggcggggt atacaaccat catgtctcat cgttcaggcg
    10081 agaccgagga tgtaactatc gccgaccttg cagtggcgac caatgccggg cagattaaaa
    10141 ccggtgctcc ttgccgagga gaacgaactg caaaatacaa ccagctgctc agaattgaag
    10201 aggagttggg tagtcgagct gtttatgccg gaaagaaagc tttcaatccc aaacgcggca
    10261 agtaaatcgc ccggctctca atccatccgc ttgatccgcc cgcggcgggc aagagaaaac
    10321 ctcaaggaaa agattgctcg gaccggcgtc atagcgaccg ggttgcgcca agcgtatcct
    10381 gaacttcggg tgccgcttga ctatggaacc acctttcagc ttctggttgc tgtcattcta
    10441 tctgcgcagt gtaccgacgc agccgtcaac aaggtgacgc cggagctttt tcggcgcttt
    10501 ccaactccgg accggatggc cagcgcacct ttagccgaga tcgagagcat gatccgtccg
    10561 cttggcttat ttcgcgccaa agccagatca ctgaaagctt gcgctcaaca gcttggcgaa
    10621 cgattcaatg gccaggttcc tggcacgatg gaggaattga ccaccttggc gggcgtcggc
    10681 aggaaaaccg ccaacgtcat cttaggtcat gcttttgatg tacccggaat catcgttgac
    10741 acccattgta aacgtgtgtc acgccgttta ggtcttaccc gcagcgaaga tccagttcag
    10801 atcgaacgcg acttgagcaa attattgccg gctgaagaat ggactggttt ttcgcaccga
    10861 ctgattatcc acggccggcg gatttgccat gctcggaagc cagattgtaa agcttgcatc
    10921 gtcgagcggc tttgcccgtc aagcagtctc atatgggacc ggccaaatcc gagaactcac
    10981 gcgcataacc gacaagttca cgggtgatcg agccagcctt cagttctaac gccatcaatg
    11041 cgtcatcccc gccgtattca ttttccaatc ctaaaaggtt cgcacttcta atgccgaacc
    11101 ggcggtaata ctcgggcgcg ccaagaacca tagtgaatcc gacgcctgct cgagtaggac
    11161 gagagcgttc atgggagaga gtggaggcag tctgtgggcc tgggtagata gacgttcctt
    11221 tgacaacgct ggtacattcg taggccgaga cgagtgttga ggtaatctct tgcgcggatc
    11281 attcggctcg gcttgagacg gattccgttc agcgcaactt ttccccatga tgccgctgcg
    11341 gcaagcgacc gtgttttgaa gaaatgcgcc gcccgaagtt acttgccaat tctgagttca
    11401 gtttcttcgg atgcctatcg ttcatggggc ggcggcttag ggccaaacga agttgggcaa
    11461 agtattattc tccagcacgc gataatttgt cagatcgacc tgtagcggtg tcaccgagac
    11521 atacccctcg tgaactgcca cgcaatcggt gccgtcttct ttatcgaagc caagatcatc
    11581 gccgcctatc caataatatt tgccgccgcg aggatcgaca cgttcgacaa tattctcgct
    11641 gtaatggcgc tttcccatgc gggttaagcg ccagccttta agctcggatt tcggcagcgg
    11701 cggcacgttg atgttgagaa gcgtttcggg cggaataccc ccggtatctt gtatgcttga
    11761 tactaagaag ccggtgaatt ccgctgcggc gcgaaaatca aaatcacgga atgtaaccaa
    11821 tgacacggca atcgacggaa ctgttagcaa ggccccttcg atagcagctg agacggtccc
    11881 ggaataaatg atgtcatcgc ccagattagg ccctttgttg atgccggaaa ccacgaggtt
    11941 cggacgaacc ggcaagagac cggtcaatgc cagtttcaca cagtcgcttg gggttccgtt
    12001 aactgccaga cgtcgcgg
//
LOCUS       DASUVS010000357        21980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1023217, whole genome shotgun sequence.
ACCESSION   DASUVS010000357 DASUVS010000000
VERSION     DASUVS010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 21980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21980
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023217"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VF182_19010"
     CDS             complement(<1..785)
                     /locus_tag="VF182_19010"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX6439218.1"
                     /translation="MKLADLYAQPGLTLSVEFFPPKTDKGEEALFGEIELIKSLRPAF
                     CSVTYGAGGSTREKTVDLVDRIHRECGLETMCHLTVVGQSKDDARGVLKRLQQKGLEN
                     VLALGGDPPAGMPNWQPHPDGFQHAVELVREAISFGYFSVAVAGFPEIHPRAKSRVSD
                     LGYLKEKIDAGAQAIITQLFFDNDQYYRYFEDVRKLGVQVPIIPGVLPIVSAPQIRRF
                     TALCSARIPPQLEQALNKVENDDNAATQLGIEYATRQCEALIQ"
     gene            complement(882..1289)
                     /locus_tag="VF182_19015"
     CDS             complement(882..1289)
                     /locus_tag="VF182_19015"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEX6439219.1"
                     /translation="MDSYFDQLGGEPELRKIIDTFIDRVFADRMIGFFFRKVDKDRIK
                     EMEYQLAASFLGASVEYQGRPLDRAHARHPIMGGHFARRKQILKETLELYEVAENVKE
                     AWLKHTEDLRPLITGQGGSGCDPIDARRKATGS"
     gene            complement(1324..1608)
                     /locus_tag="VF182_19020"
                     /pseudo
     CDS             complement(1324..1608)
                     /locus_tag="VF182_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335502.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
     gene            complement(1667..2221)
                     /gene="sufT"
                     /locus_tag="VF182_19025"
     CDS             complement(1667..2221)
                     /gene="sufT"
                     /locus_tag="VF182_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HEX6439220.1"
                     /translation="MQSEQPVIVKRDCDVLMIPSGEKSTLASGSTVWLTQALGGSFTV
                     MTDRGYMVRVEGKDADAIGLEPILDGKSGDAYSDNDIEVIESRVWEQLRSCFDPEIPV
                     NIVELGLIYDCSVTPSENGYRAGVKFTLTARGCGMGEVLKRDIAAKLLAIPGVKQADV
                     ELVWEPPWSQSRISAAAKLQLGMA"
     gene            complement(2249..2704)
                     /locus_tag="VF182_19030"
     CDS             complement(2249..2704)
                     /locus_tag="VF182_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014706737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX6439221.1"
                     /translation="MSELDELYQEVILDHNKSPRNFREMADANRRSEGYNPLCGDHVT
                     VYVNLADGVIRDISFKGAGCAISKASASMMTTELKGKSEAEAEEVFDSVHKMLTGEMG
                     VDESDFERLGKLKILSGVCKFPARVKCASLAWHTVNAALKGTGEVASTE"
     gene            complement(2704..3966)
                     /locus_tag="VF182_19035"
     CDS             complement(2704..3966)
                     /locus_tag="VF182_19035"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX6439222.1"
                     /translation="MKAVREKRLPRSGNESFDVEKIRRDFPILKERVHGKPLVYLDNG
                     ATSQKPQVVIDTLDRYYKSENSNIHRGVHYLSERATSSYEASREKIRQFINAENGHEI
                     VFVRGTTEAINLVAQSYGRTFLKAGDEIVISAMEHHSNIVPWQMLCGQIGARLRVVPI
                     NHDGEFVLDEYRRLLSENTKLVSLTHISNALGTIVPVKEAVRLAHERGVPVLLDGAQA
                     VPHLKVDVRDIGCDFYAFSGHKLFGPTGVGVLYGKSELLDAMPPYQGGGDMISLVTFE
                     KTHYNVLPYKFEAGTPNIAGGIGLGAAIDYLNGFNWEAVCAHENHLMAYATNALSEIP
                     SIRIIGTAKDKAGVLSFVFDHIHAHDVGTILDQEGVAIRAGHHCAMPVMQRFGVPATA
                     RASFALYNNREDVDALVRGIHRVLKVFS"
     gene            complement(3972..5291)
                     /gene="sufD"
                     /locus_tag="VF182_19040"
     CDS             complement(3972..5291)
                     /gene="sufD"
                     /locus_tag="VF182_19040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX6439223.1"
                     /translation="MVNSHTQQFLSAFERFQQKSETTPEWLRNVRRAAIDDFVQLGLP
                     TTRHEDWRYTSVEPITSTGFGVANGEAKLLRAEDLYGPSFIDPAYNRIVFVNGVFCPQ
                     FSSIRELPAGVQAKNLSAVLATNEANVRPLLTQKSTSRDQAFVALNTAFAAEGAFISI
                     PKSLALGEPIYLVFCSFGSGNPIMSHPRNLIVFEAGSQARVVESYIGLGDQPTLTNVV
                     TEVVGGAGSVIDYYRLQNESSSGYLIAALEADISRDCSFTAHSVTLNGSLIRNDIHVV
                     LDGEGAECVLNGLYLLDGKQHVDNHTEIEHAKPRATSRELYKGILSGAAHAVFNGKIL
                     VHKDAQKTDARQTNKNLLLSEQAVVNTKPQLEIHADDVKCSHGSTIGQLDKDALFYLR
                     SRGLDGAEAQSLLSYAFASDLVGRIRIAPLRARLDDYLLTKFRRSQG"
     gene            complement(5322..6080)
                     /gene="sufC"
                     /locus_tag="VF182_19045"
     CDS             complement(5322..6080)
                     /gene="sufC"
                     /locus_tag="VF182_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX6439224.1"
                     /translation="MLEIKNLRATVGGQEILRGINLTIGAGEVHGIMGPNGSGKSTLA
                     HVLSGRENYQVTEGEILYNGKNLLEMSPEERAREGIFLAFQYPVEIPGVSTTYFLKAA
                     LNAIRKHQGLDELDAMEFLSLIKEKMKLVEMDQALLNRPLNEGFSGGEKKRNEILQMA
                     VLDPKLAILDETDSGLDIDALRIVAGGVNALRQEDRAMVVITHYQRLLNYIVPDYIHV
                     LYDGRIVKSGSKELALELEVKGYDWIKAEAAAAQ"
     gene            complement(6101..6553)
                     /locus_tag="VF182_19050"
     CDS             complement(6101..6553)
                     /locus_tag="VF182_19050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX6439225.1"
                     /translation="MNVGRRVDYAVRALAYLAGQPSGKIVSRADIEKSQDIPSFYLSK
                     IMKDLVADGLVSSHIGSKGGFTLARPPNTISIKNVYEAVERPLVLMECLEKGADYCSY
                     CSVCSQKSIWEEAQALLAKFLGGVSIREIADRHGLKERLEKAAIQRVG"
     gene            6713..7246
                     /gene="def"
                     /locus_tag="VF182_19055"
     CDS             6713..7246
                     /gene="def"
                     /locus_tag="VF182_19055"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX6439226.1"
                     /translation="MSILKVARLGHPVLRKITEAVSEKELRSETTRQFVDDMIETMKE
                     YDGVGLAADQVHVSKQIAVLEVADNPRYPEKPLVPLTVLINPLITPLTEEFEDDWEGC
                     LSIPDMRGKVPRYKSIRVQALDRNGAPLDFVANGFHARVVQHEWDHLNGKVYLDRMSD
                     FSTLSFITEFARYWLGK"
     gene            7301..8593
                     /gene="cofH"
                     /locus_tag="VF182_19060"
     CDS             7301..8593
                     /gene="cofH"
                     /locus_tag="VF182_19060"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HEX6439227.1"
                     /translation="MNFPQLQESFDRLMSMVNPVIGGILDRALSGADISADEATRLFE
                     ADGADLPAMILAANHLRAETVGDVVTYVVNRNINFTNVCVKACGFCAFSRGHLAEEGY
                     FLPIEEIIRRGEEARELGATEICVQAGLAPGIDGRHYIKLCRALKEALPDIHIHGFSP
                     EEVLYGSSLSGLSVRDYLKALKDAGVGSLPGTSAEILVDDVRQQLSPGRITTAQWIEL
                     IQSAHEMGIPTTSTIMYGHLETAHHKATHLLILRDIQKHTGGITEFVPLSFVYEEAPI
                     SRRNKIAGLRPGASGSEVMKMYAVSRLMLNKWIPNLQVSWVKEGPKLSQIALLAGAND
                     FGGTLINESISTAAGASYGQLMKPSQFRGLIREMGRIPAERSTTYKDIKVFGTENGSV
                     DLLDQVNDPSERFGSYRNLIALKNFRFKEFYSEQKHKH"
     gene            8638..9282
                     /locus_tag="VF182_19065"
     CDS             8638..9282
                     /locus_tag="VF182_19065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439228.1"
                     /translation="MQGNPASFDAIGNRLGFGIAENNLQRLRLVWHGPRFPAYLCLSI
                     AIVLLFISVPIVEAIRLRGFAGPAASLWYFPLMNAILFGIACYLLTLKRSILFDHLAQ
                     EIVLTNQSLFKRSLLSASYSEIEQINLGLDQVYNGFAVAGSSAAQQFPVPSLRLFLVN
                     GDSVLLDRGSRGKLKELGNQIGERLKRPFEIDVRLGLQKMAVVKAHEEAERARI"
     gene            complement(9252..9881)
                     /locus_tag="VF182_19070"
     CDS             complement(9252..9881)
                     /locus_tag="VF182_19070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439229.1"
                     /translation="MLKVLALALTLLAGCSFGSGNLQSKHSNDLESRRIRRIAVLPPE
                     TSNAAGQALPQQSGGPEMLAKQVYSAMASLPNWQIVAESEVKQVEQITPAAPGPARLR
                     QIGEMVFADAIMVGRIERFRERVGDDWGVKSPASVAFVLDLIDVRRGDVIWSARFDET
                     QKPLSENIFALGDIGQRGVRWLTAEQLAIDGVKKAVGQLHQILARSASS"
     gene            complement(9932..11734)
                     /gene="aspS"
                     /locus_tag="VF182_19075"
     CDS             complement(9932..11734)
                     /gene="aspS"
                     /locus_tag="VF182_19075"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006006894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX6439230.1"
                     /translation="MAIERVFCDQLGDWKRSCYCGEPRAGAVGRELVLTGWVHTRRDH
                     GGLIFVDLRDRSGITQIVFNPEVDLCAHEKAKQLRSEDVIAVRGVLSRRPAETLNPDL
                     ATGEVELAGNELRLLNASQPPPFVIDDETDANENTRLKYRYLDLRRPRSLAHLLLRYR
                     MTKLIRDYLDERGFIDVETPVLTKSTPEGARDYLVPSRIYKGKFYALPQSPQLFKQIL
                     MVGGIDRYFQIVKCFRDEDLRADRQPEFTQLDMEMSFVQADDVISIVEGMIVLLLREL
                     KGIDIGRPFPRLTWTEAMSRFGSDKPDMRFGLELQDFTDALRGSNFKVFAAAIAKGGV
                     VKGIRIPRGIELSRKDLDDMTPFVATFGAKGIAWTRIADEGWQSPIAKFLSEAEKREI
                     ERIAEAQLGDIILFSADTARTVCDALGNLRLYLGEKFGLIRENEYALVWVLDFPLMEY
                     DAEEQRYVALHHPFTAPLDEDLSLLDTEPAKVRSKAYDLALNGTEIGGGSIRIHQLEL
                     QKKILGLMGIGAEEAQAKFGFFLEALSFGAPPHGGIAFGIDRLAMLLSGAQSLRDVIA
                     FPKSQRAVCLLTEAPSTVDARQLRELGIRIKAAE"
     gene            complement(11735..13000)
                     /gene="hisS"
                     /locus_tag="VF182_19080"
     CDS             complement(11735..13000)
                     /gene="hisS"
                     /locus_tag="VF182_19080"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX6439231.1"
                     /translation="MKVTAIKGFSDILPDEVEFWQRVEKEARKIFTAYNFAEIRIPIV
                     EKTELFNRSLGETTDIVEKEMYTFADQDAKSSLLTLRPEGTAGVVRAYVESELFKTEP
                     VQKLYYLGPMFRRERPQKGRMRQFHQIGAEVLGRSDPFIDAEVLLLLNDYFSAIGLEG
                     AALQLNSLGCSECRPKYRQRLLAFLTEREEALCANCRRRMERNPLRVLDCKEPGCIEQ
                     TKYAPSILESLCDPCRRHFETVQRLLRQSGMAYDLNPRMVRGLDYYCRTTFEWTSNQL
                     GSQSAIAAGGRYDGLVQELGGPPTPGVGFAIGVERLTMLLRSKTSSALSGPAVYIVWV
                     GENAQQWAFPVVHRLRQNGLSIELEGEVRSLKSQMRRADKLNASSVLIIGDNELEKGV
                     ALLRDMATRQQSEIGLNDVEEALLSRKAN"
     gene            complement(13233..13955)
                     /locus_tag="VF182_19085"
     CDS             complement(13233..13955)
                     /locus_tag="VF182_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439232.1"
                     /translation="MSTVQATLWLYATALRRSWESLLKNWVVSLAPWAYAALLSIVSL
                     MVAPLGMIGGLVLGLATQACISSALYLVKNIVEGRRADFKDFINGFTVYLWELLGIAF
                     ILWIPMRVLAMALASTPNGGLLFALIQVAVYILLNPAPELIYQTRSSGIGVISDSYNF
                     VVENWLEWFLPNIVLTLAGYCIFNILSAISIGLPGVVQIFIVAVGMGLCLSYFMIFRG
                     FLFAELHGSTRRSRAYRYKMQG"
     gene            14589..14816
                     /locus_tag="VF182_19090"
     CDS             14589..14816
                     /locus_tag="VF182_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439233.1"
                     /translation="MIYIVTYDETRYHRQNVKRLLLVESNLGLELERVTGLVRMLTDG
                     NFAAASVEIAGQRDWDARRVRHPAIRSYKLD"
     gene            15423..16022
                     /locus_tag="VF182_19095"
     CDS             15423..16022
                     /locus_tag="VF182_19095"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439234.1"
                     /translation="MHTRIKITFLASLVVMLLWRALPIQAGAPTEEIRTAIDQGVQIL
                     KSAKLDNSKQRAQVIDQLREIVYTRFDFEEMAKRSLGSHWRRLNPTQQKEFVTAFTDL
                     LETTYADKIDLYEGQQVEYTGETIDKNYAEVNTKVIGKNRESYSVDYRLHQAGGKWRI
                     YDVVAENISLVNNYRSQFNRIVVNSSFEELIKRIKQKSS"
     gene            complement(17006..18028)
                     /locus_tag="VF182_19100"
     CDS             complement(17006..18028)
                     /locus_tag="VF182_19100"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6439235.1"
                     /translation="MKKLIMNTAIIACICFFSRPVSAQNQFYEGKTIRIIVGFSAGGG
                     YDALARMLSRHMPKYIPGHPTIVVDNMTGAGSLLAANHIFNVAKPDGLTFGHFSGGFA
                     FNQVMGQPGIEFDARKFVFVGAVARDESAIALTKASGINNMEQWMAAKTPVKMGTTGP
                     GAFGTDNVIKVIKAALNLPIQIISGYKGTAEMRLAAESGEIDGTTWGWDTMRGAWQKA
                     IEAGTVVIVLQTVPKPFPDLPRVPLAIDLAKTPEAKQLIEVGIHYPSKITKTLALPPG
                     TPTDRAQILQKALQGTVKDKEFIAEAEKAKLGLAPVTAEDMKSTVDGIFKLDADILAK
                     LKTILF"
     gene            18176..19438
                     /locus_tag="VF182_19105"
     CDS             18176..19438
                     /locus_tag="VF182_19105"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6439236.1"
                     /translation="MTKYSETNSARDPAAIIRPWSSLLIRDYRLIFIAILCANTSMHM
                     RNVASLYHVYHLSGSSVQLGLAGFFQALPFIFFGLFGGVLADTYNRKKLILFTAAFNL
                     LPGLALGLLTTTGVIQVWHINTLNVVTSSLQVLGGPSRQAIIPSLVPQSYLLNAVTLS
                     TLMMQGTQLTAPVFAGFMIDFFGVDASYIADALLLMPAICCVLLIRSSGQPHGARRQV
                     SWRSLIEGFEFLYHTRIILSLFLLDFFAVLFGFYRPILPIFADDVYQVGARGLGALYA
                     APAIGALFGSGMLLALGDIKRKGALSIAATLLFALSLGFLGLSRWFWLGLIAVGVLGL
                     ADAISVTMRRTVVQILAPDDMRGRATSFLTVFAQTTNALGAVIAGGGAALLGAPNAAL
                     VGCILCTLTVLAVCWAIPQLWSYRSDTH"
     gene            19569..21527
                     /locus_tag="VF182_19110"
     CDS             19569..21527
                     /locus_tag="VF182_19110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HEX6439237.1"
                     /translation="MNSNRISSQEKALHNLQSVLDPVRALAPSADLSSLERARSLVAS
                     KFSENEQARASIKRGAEAVERLGKLGMDAETLAAALLLPLVEEGVLDPAMTEKSIGAE
                     VARLTQGALRLASLKDLRKTAGESLQANRLRQMLLTIAEEPRVVLVSLAHQLCALRAA
                     SHAPEQIRRALAQDSLEVFAPLANRLGVWELKQELEDLAFRYLEPDTFRRISDGLDQR
                     HTDRERYIAQVVARLREELFRAGINAEISGRPKHVYGVWNKMRQKGLEISDVFDVLAF
                     RMVLNDVSDCYAALGVVHGLWEPILDAFDDYIAKPKANGYQSLHSAVIGPEGVTIEVQ
                     IRTVAMHRHADLGVAAHWQYKEGLPRRIAWSRGFLEAAQSTSDGVDIIDRFKQAAFHD
                     RIYVLTPQGHIVDLPAGATPLDFAYAIHTQVGHRCRGAKVDGVIVSLTTPLASGQQVE
                     ILTARAGRPSRDWLNPNLGYLKTASARSKVRRWLTEQDHEAHVAQGRTVLEREHRRLG
                     LSEINIAELTKRFEMRRPEDLLAALGRGDISIVRLGYALGIQVPPPVPSTPASSPQPS
                     SRASLLVHGASNVLTKIAGCCRPVPGEPIGGYVTAQGGGISIHQRDCRNLKQLAELRP
                     HKLVEVAWGEISGQRPAVAGMPRKPVSD"
     gene            complement(21701..>21980)
                     /locus_tag="VF182_19115"
     CDS             complement(21701..>21980)
                     /locus_tag="VF182_19115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439238.1"
                     /translation="ALNACCAKGALREVCVVLCADAKEGQRNGVGVMVVYRGRKFGLR
                     SALALSRAWQLRKRCPARRAFLFSRMPMYRNVSPGIMGSWDERSGPVK"
BASE COUNT         5134 a         6095 c         5573 g         5178 t
ORIGIN      
        1 aactggatca acgcctcgca ttggcgtgtc gcatattcga tccccaattg agttgccgcg
       61 ttatcgtcgt tttcaacttt gttcaacgct tgctccagct gcggcggaat tcgcgcgcta
      121 cataaagccg tgaagcggcg aatttggggc gcggaaacga tcggcagaac gcccggaatg
      181 atcggcactt gaacacctag tttcctgaca tcttcgaaat agcggtaata ttgatcgtta
      241 tcgaaaaata attgggtgat gatcgcttga gccccagcat caattttttc cttcaggtag
      301 cccagatcgg agaccctgct tttagctcgc ggatgaatct ccggaaagcc tgccacggca
      361 accgagaagt agccgaaaga aattgcctct cgaaccagtt ccacggcatg ctgaaaacca
      421 tcgggatgcg gctgccaatt cggcatcccc gccgggggat ccccgcccaa agccaggaca
      481 ttttccaaac ctttttgctg aaggcgcttg agaactccgc gagcatcatc cttggattga
      541 ccgacgacag tcaaatgaca catcgtctcg agaccgcatt cgcgatgaat tcgatccacg
      601 agatcaacgg ttttttcgcg cgtgcttccg cccgccccgt acgtaacaga acaaaaagcg
      661 ggtctcagcg atttgattaa ttcgatctcg ccgaacaggg cttcctcgcc tttgtctgtt
      721 tttggaggaa aaaattcaac tgagagtgtt aacccgggct gggcataaag gtcagctaat
      781 ttcactaagg aatattcctg atgaataccg tagccggatt gactactaat aacacaaggt
      841 tataagggat cacaaccggg atggcgaggg aagggaaagt ttcaggagcc ggttgccttg
      901 cgccgcgcgt cgatgggatc gcacccggag ccgccctgac ctgtaattag aggccggaga
      961 tcttctgtat gttttaacca cgcctctttg acgttctcgg caacctcata caactccaag
     1021 gtctctttta aaatctgctt gcggcgcgcg aaatgacctc ccatgattgg gtgccgggca
     1081 tgcgcccggt ccaacggccg tccctgatat tctacactgg ctcctaggaa actggccgca
     1141 agttggtatt ccatctcctt gatgcggtct ttgtcgactt tgcgaaagaa aaacccgatc
     1201 atccggtcgg caaagacccg atcgatgaac gtatcaatga tctttctaag ttccggctca
     1261 ccgccaagct ggtcaaagta actatccata taaaaacctt gattaaacaa tcccggattg
     1321 tgcctacatc atacccagct gaagccgtgc cgcttccgac atcttgcttg gatcccaagg
     1381 cggatcccac actaaatcaa ttttgacatc agtcactccg gagagagcct tgaccttctc
     1441 ttcgatttcc gccggcatag actgagcggc gggacaatgg ggcgaggtca gcgtcatagt
     1501 gattgttacg gcacccccct cttcaacgtt gacgttataa atcagcccga gctcgtaaat
     1561 gttaacggga atttctgggt cgaaacaggt tctcagcgcc tcgagcactt cgctttcgat
     1621 aaccagctcg ttgatacttt ccatatttta ctccggaact aggctgctat gccatgccca
     1681 actgcagctt cgcagctgca gagatgcggc tttgactcca aggcggctcc cagaccaact
     1741 cgacatcggc ttgttttaca cctggaatgg caagcaattt ggccgcgatg tctcgcttca
     1801 aaacttctcc catgccgcat ccgcgagcgg ttagggtaaa tttaacgcca gctcgatacc
     1861 cattttctga aggcgtcacc gaacagtcgt aaatgagccc tagctcgaca atattgaccg
     1921 ggatctcagg gtcaaaacag gaccttagct gttcccaaac acggctctcg attacttcga
     1981 tgtcattgtc tgaataggcg tcgccggatt taccatcgag gatcggctcc aggccgatgg
     2041 catccgcatc ttttccctcg acacgaacca tataaccccg gtcggtcatt accgtgaaac
     2101 tgccgcccag agcctgagtt agccagactg tgctaccgga ggctaaggtt gacttttctc
     2161 cgctaggtat catgagcaca tcacaatcgc gcttcacgat cacgggttgc tcggattgca
     2221 tagcatgctc ctcctgcgcg aaattgcatc actctgttga tgcaacctcg ccggttccct
     2281 tcagtgccgc attcacagta tgccaagcca aactggcgca tttcaccctc gctggaaatt
     2341 tgcaaactcc cgacaatatt ttcagtttgc cgagccgttc aaagtcactc tcgtccacgc
     2401 ccatctcgcc tgtgagcatc ttatggacac tgtcgaacac ttcttccgct tcagcctccg
     2461 atttgccctt cagctcggtg gtcatcatcg aagccgaagc cttggagatg gcgcagccgg
     2521 cccccttgaa gctgatgtcc cgaatcactc catctgccaa gttcacatag actgtcacgt
     2581 ggtcgccgca caacggattg taaccctcgg atcgacgatt ggcatccgcc atttcgcgga
     2641 aattgcgggg actcttgttg tgatccaaga tcacttcttg atagagctca tccaattctg
     2701 acattaacta aacaccttca acacccgatg gattcctcgc accagcgcat cgacatcctc
     2761 ccgattgttg taaagggcaa acgaagcgcg cgccgtcgcc ggcacaccga accgctgcat
     2821 caccggcatg gcgcaatgat gtcccgcccg tatcgccact ccttcttggt cgagaatggt
     2881 accgacatca tgggcatgga tatgatcgaa aacaaatgac agaaccccag ctttgtcttt
     2941 agctgtgccg attatcctga tcgacggaat ctccgacaaa gcgttcgttg cataagccat
     3001 tagatggttc tcgtgcgcgc acaccgcttc ccagttaaaa ccgttcaaat agtcaatggc
     3061 cgcgcccagt cctatcccgc ctgcaatatt gggcgtccct gcttcgaact tatagggcag
     3121 cacgttgtag tgagtcttct cgaaagtcac aaggcttatc atatcgccgc cgccttgata
     3181 cggcggcata gcgtctagca gctcggactt tccgtaaagg acccctacac cggtaggtcc
     3241 aaaaagcttg tgaccggaaa aggcgtaaaa gtcgcagccg atgtcccgaa catccacctt
     3301 caaatgcggc accgcttgcg cgccatccaa taacaccggc acgcctcgct cgtgcgccag
     3361 ccgcacggct tctttcacgg gcacaatagt tcccaaagca ttggagatat gtgtcaggga
     3421 aactagtttg gtattctcgc tcaatagacg gcgatattca tcgagaacaa actcgccatc
     3481 gtgattgatc ggcacaacac gaaggcgcgc gccgatttgg ccgcataaca tttgccatgg
     3541 cacgatgttg gaatggtgct ccatggctga gatgacgatt tcatcgccgg cttttagaaa
     3601 agtccttcca tagctttgcg cgaccagatt gatggcttcg gtggtccctc ggacgaaaac
     3661 gatctcatgc ccattttcgg cgtttatgaa ctgacggatc ttctcccgtg acgcttcata
     3721 actggaggtt gcccgctcgc tcaggtaatg aacaccgcgg tgaatgttgg agttttccga
     3781 cttgtaataa cgatcgagag tgtcgatcac aacctgcggc ttctgactgg tagcgccatt
     3841 atcaagatac accagcggct tgccatgaac ccgctctttg agaattggaa aatcgcgccg
     3901 aatcttttcg acatcgaaac tttcattccc tgaacgcggc aatcgttttt cccgaacagc
     3961 tttcatggtt cttagccttg gctccgtcta aactttgtaa gcaaataatc atccagccgc
     4021 gcccgaagcg gtgcgatgcg gatgcgccct acgagatcgc tcgcaaatgc gtagcttaga
     4081 agactctggg cctcggctcc atcgagaccg cgcgacctga gataaaaaag cgcatctttg
     4141 tcgagctgtc cgatggtcga gccgtggctg catttcacgt catcggcgtg gatctcgagc
     4201 tgcggcttcg tgttcaccac tgcctgttca gagagtaaca agtttttatt ggtctgccga
     4261 gcatcggttt tctgagcatc tttatgtacc agaatcttgc cgttgaacac tgcgtgggca
     4321 gccccgctga gaattccttt atacagttcc cggctagtcg cgcgcggctt ggcgtgttca
     4381 atttcggtgt gattgtcgac atgctgtttg ccgtcgagca gatagagacc gtttagaaca
     4441 cattcggccc cctcgccatc aaggaccacg tgaatatcgt tgcggatgag tgaaccgtta
     4501 agagtcaccg aatgggccgt gaagctgcaa tctcggctga tatccgcctc caaagcggcg
     4561 ataagatagc cggaactcga ttcgttttgc agacggtaat aatcaattac cgaccccgct
     4621 ccaccgacga cctcggtaac aacgttggtc aaagtcggct gatcgcccag accgatatag
     4681 gattcaacaa cacgcgcctg gcttcccgct tcgaaaacaa tcaaattccg cgggtgcgac
     4741 ataattggat tgccgcttcc gaacgaacag aataccagat aaatcggttc gcccagcgcg
     4801 aggctttttg gaatcgagat aaacgcgcct tctgcggcaa acgccgtatt cagagcaacg
     4861 aacgcctggt cgcgtgaagt ggatttctgc gttagaagcg gcctgacatt agcttcattt
     4921 gtcgccaata cggctgaaag gttttttgcc tgcacgccag ccggcagctc gcggatggat
     4981 gaaaattgcg ggcaaaaaac gccgtttacg aagacgattc gattgtacgc cgggtcgatg
     5041 aaagacggcc cgtagagatc ctccgcgcgc aatagtttgg cctctccgtt cgctaccccg
     5101 aaaccggttg aagtgatggg ctctacactg gtatagcgcc agtcttcatg acgtgtagtt
     5161 ggaagtccta gctgaacgaa gtcgtcaatg gcagctcgtc gcacatttcg cagccactcg
     5221 ggcgtcgttt cggatttctg ttgaaaccgc tcaaaagcag aaaggaattg ctgggtatgg
     5281 gaattgacca tattactaat taatccaaag tttcgaaccg gttattgcgc agccgccgct
     5341 tcagctttga tccagtcgta gcctttcact tcaagctcaa gcgccagttc cttgctgccg
     5401 gattttacga tccgtccgtc atagaggacg tggatatagt ctggcacgat gtagttcaac
     5461 aagcgttggt agtgcgtgat cacgaccatc gcgcgatctt cttgtcgtag cgcattgacg
     5521 ccgccggcga cgatccggag cgcatcaatg tcgagtccgg aatcggtttc atcgagaatt
     5581 gccaactttg gatccagtac ggccatctgc aaaatttcat tacgcttctt ctcgccgccg
     5641 gagaatccct cgttaagcgg ccgattgagc agagcttgat ccatctcaac cagtttcatc
     5701 ttttctttga ttaaggaaag aaactccatc gcatccaatt catctagtcc ttgatgcttg
     5761 cggatggcgt tcaaagcggc tttaagaaaa taggtcgtgc taactccagg aatctctacg
     5821 gggtattgaa aggccaggaa aattccctcg cgtgctctct cctccgggct catctccaat
     5881 aggttctttc cgttgtacag aatctcgccc tcggtgactt gataattctc ccggcccgac
     5941 aggacgtgag ccaacgtgct cttgcctgat ccattgggtc ccataattcc atgcacttcg
     6001 ccggcgccga ttgtcaggtt gataccgcga agaatttcct gcccacccac cgtcgccctg
     6061 agattcttga tttccaacat cgcgattcct tccaactaga tcagccaact ctctgaattg
     6121 ctgctttttc caggcgttcc ttgaggccgt gccggtcggc aatctcacga atcgaaactc
     6181 cgccgagaaa cttagccaat agcgcctggg cttcttccca aatcgatttt tgcgagcaca
     6241 ccgaacaata cgaacagtag tcggccccct tttccaagca ttccatcaag actaatggtc
     6301 gctcaacagc ttcgtaaacg tttttgatgc tgattgtgtt cggcggcctc gccagggtga
     6361 atccgccttt cgagccaatg tgcgaagaga ccagtccgtc ggcgacgaga tccttcatga
     6421 tttttgaaag atagaaggat gggatatcct ggctcttttc tatatcggcc cggctgacga
     6481 tcttgccgga aggctgcccc gctagatagg cgagcgcgcg aaccgcataa tccacgcgtc
     6541 gtccgacgtt catcaacgag ctgcgctcgc ttctcgccct cgaattcttc atgctctgct
     6601 ttgaatatag actcactagg tcttttatgt caaggtagcg cactcaagga ttgttcgatt
     6661 ggtgacacgc ttccgcccgc gccgtctctg tgctaaatcg tgattgaatc ctatgtctat
     6721 tcttaaagtt gcgcgtctgg gccacccggt tttacgaaaa ataaccgaag ccgtttcgga
     6781 gaaggaactc cgctccgaaa ccactcgtca attcgtcgat gatatgatcg aaaccatgaa
     6841 ggaatacgat ggcgtcggtc tggccgccga tcaggttcac gtgtcgaaac agatcgcggt
     6901 ccttgaggtg gccgacaatc cgcgttaccc cgaaaaaccg ctggtgccgt tgactgtcct
     6961 tatcaatccc ctcatcacac cgcttaccga agagttcgaa gatgattggg aaggctgtct
     7021 tagcattccc gatatgcgag gcaaggtgcc gcgctacaaa tccatcaggg tccaagcctt
     7081 ggaccgtaac ggcgcgcctt tggacttcgt tgccaacgga tttcatgccc gggtagttca
     7141 gcatgagtgg gatcatctga atggtaaagt ctacctcgac cgcatgtccg acttttcaac
     7201 cctcagtttc atcaccgagt ttgcccgcta ttggcttggt aaatagctgc gatggactcg
     7261 agtgattgtc tttgaaaaac tgaaaggata gagtcgcgcc atgaatttcc cacagctaca
     7321 ggagtctttt gatcggctca tgtcgatggt caacccggtc atcggaggca tactcgatcg
     7381 cgcgctctcc ggggcggata tttccgctga cgaagccacc cggttgttcg aggcagatgg
     7441 cgccgatctc ccggcgatga ttttggcggc caatcatctg cgcgccgaaa cggtcggcga
     7501 tgtcgtcacc tatgtggtta atcgtaatat caactttacc aacgtttgcg ttaaggcatg
     7561 cggtttctgc gcatttagcc gaggccatct agctgaagag ggctatttcc tgccaattga
     7621 agaaatcatc cggcgcggcg aggaggcgcg tgagttaggg gctacggaga tttgtgtgca
     7681 agccggttta gcacccggga ttgacggccg gcactacata aaactttgcc gcgcgctaaa
     7741 agaagctcta cccgacattc atattcatgg attttctccc gaagaagtgc tgtacggctc
     7801 gagcctctcc ggtctctcgg ttcgcgacta tctcaaagca ttgaaagatg cgggagtggg
     7861 aagcctgccc ggaacctctg cggaaattct cgtcgacgat gttcgccaac aactctctcc
     7921 ggggcgaatc acgacggcgc aatggattga actgattcag agcgctcacg aaatgggaat
     7981 cccaaccaca tcgacgatca tgtatgggca tttggaaacg gcgcatcata aggccaccca
     8041 cctcctcatc ctccgtgata tacaaaaaca taccggcggg attaccgagt ttgtgccgct
     8101 gagctttgtt tacgaggaag ccccgatctc tcgtcgaaac aagatcgccg ggctccgtcc
     8161 gggcgccagt ggctccgaag tcatgaaaat gtacgcggta tcgagattga tgctgaataa
     8221 atggataccg aacttacaag tctcctgggt caaagaaggg cccaagttat cgcagattgc
     8281 tttgctggca ggcgccaatg attttggcgg caccctgatc aacgaaagca tttcgaccgc
     8341 ggccggagcg agctatggac aactgatgaa gccgtcacaa ttccggggtt tgatccggga
     8401 aatggggcgc atacccgctg aacggtcaac tacgtataag gatataaagg tatttgggac
     8461 tgagaatggt tctgtcgatc tgctcgacca agtgaacgac ccgtccgaac gtttcggatc
     8521 ttatcgcaac ctgattgctt taaaaaattt tcgcttcaaa gagttctata gtgaacagaa
     8581 acacaagcat taagcctttc cgggcaaacc ccgatacttc taaaggccgc tcccggcatg
     8641 caaggtaatc ctgcgagttt cgacgcgatt ggaaaccgtc taggtttcgg catagcggag
     8701 aacaatcttc aacgtttgcg actggtctgg cacggtccga ggtttccggc ttatctctgt
     8761 ttgagcattg ccatcgtctt gctgtttatt tccgtgccaa tcgtcgaggc cattcgcctg
     8821 cgcggcttcg ccggcccggc agcctctctg tggtatttcc ctctaatgaa tgcgattctt
     8881 ttcggcattg cttgttactt gctaacgttg aaaagaagta ttttgttcga tcacctggcg
     8941 caggaaatag ttctaacgaa ccaaagcctc ttcaagcgat cattgctgtc tgccagctat
     9001 tcggaaatcg aacaaatcaa tctgggcttg gatcaagtgt acaacggctt tgccgtcgcc
     9061 ggatcgtcag cggcacaaca atttcccgtt ccgtcgttgc gcttgttcct ggttaatggt
     9121 gacagcgtac tgttggaccg tggcagccgg ggaaaattga aagaactcgg caatcaaatc
     9181 ggcgagcgat tgaaaaggcc tttcgaaatc gatgtacgat tggggctcca gaaaatggcc
     9241 gttgtgaaag ctcacgagga agccgaacgc gccagaatct gatgtagttg tccaacggct
     9301 tttttgaccc cgtctatagc caactgttct gccgtcaacc agcgtacacc gcgttggccg
     9361 atatcaccca aagcaaagat attttcactg agaggtttct gtgtttcgtc gaatcgcgcc
     9421 gaccagatca cgtcgccgcg ccgaacatcg ataagatcta ggacgaatgc aactgacgcg
     9481 gggcttttga caccccaatc atcgccgacg cgctcgcgga accgctctat ccttccgacc
     9541 ataattgcat cagcgaaaac catctcgccg atttgccgca gacgcgccgg acccggtgcg
     9601 gctggcgtta tttgctcgac ctgcttaacc tcgctttcag cgacaatttg ccaatttggc
     9661 aaggaagcca ttgccgaata aacctgtttg gcgagcattt cggggccgcc gctctgctgc
     9721 ggcagcgctt ggccggcggc attcgacgtt tccggtggaa gcacagcgat acgcctgatc
     9781 cgacgagatt ccaagtcgtt ggagtgctta ctttgcaggt ttcccgatcc gaagctgcag
     9841 ccggcaagca atgtaagagc aagtgcaaga acctttagca tgttaatccg tcaccctcaa
     9901 tgtcaatact gaacactcac ttggagcggc gttattcggc cgccttgatt cgaataccca
     9961 attcgcgaag ttgccgggca tccactgtag atggcgcttc cgtcaacaag cagaccgctc
    10021 gctgactctt tgggaaggca atgacatccc gcagagattg ggctccgctt agaagcatcg
    10081 ccaaacgatc aatcccgaac gcaatgcccc catgaggcgg tgcgccgaag ctcaaggcct
    10141 caaggaaaaa tccgaacttg gcttgagcct cttccgcacc gattcccatt aggccaagga
    10201 tcttcttttg caactccagc tgatgaatac ggatgctgcc gccgccgatt tccgttccgt
    10261 tcaatgcaag atcataggct tttgagcgta cttttgccgg ttcggtatcg agcaacgaca
    10321 aatcctcatc gagaggggcg gtaaacggat ggtgcaacgc aacgtaacgc tgttcttcgg
    10381 catcatattc cattaacggg aagtcgagta cccagaccag cgcgtattca ttttcgcgga
    10441 tcaggccgaa cttctcacct agatacagcc gcaggtttcc cagggcatca cagaccgtcc
    10501 ttgccgtgtc agcggagaac aaaatgatgt cacccaactg agcctcggca attctctcaa
    10561 tctctcgttt ctccgcctcc gatagaaact tggcgatcgg cgattgccac ccttcatcgg
    10621 cgattctcgt ccatgcgatc ccctttgcac cgaacgtcgc cacaaacggc gtcatatcat
    10681 ccaggtcttt gcgcgacagc tctatgcccc ttggaatgcg aatacctttg actacgccgc
    10741 ctttggcaat ggccgcggca aacactttga aatttgaccc gcgcagcgca tcggtaaagt
    10801 cctgcagctc cagaccgaag cgcatgtccg gcttgtcgga gccgaaccga ctcatcgctt
    10861 ccgtccaggt caggcgcgga aagggtcgtc cgatatcgat acctttcaat tccctgagaa
    10921 gcagaacgat cattccttcc acgatgctga tcacatcgtc cgcctggaca aaggacattt
    10981 ccatatcgag ctgagtgaac tcgggctgcc ggtcggcgcg gagatcttcg tcgcgaaaac
    11041 atttgacgat ctgaaagtaa cgatcgatgc cgccgaccat caggatctgt ttgaatagtt
    11101 gcggcgattg tggcagcgcg tagaatttac ctttgtagat acggctcggc accagatagt
    11161 cacgcgcccc ttccggagtg ctcttggtca gcactggagt ctcgacatcg atgaaaccgc
    11221 gctcatccaa atagtcacgt atcaacttgg tcatacgata acgtagcagc aggtgagcca
    11281 aactgcgtgg ccgtcgtaag tcgagatacc ggtacttcaa ccgggtgttc tcgttagcgt
    11341 cggtttcgtc atcgattaca aacggaggcg gctgtgaagc gttgagcagc cgcaattcgt
    11401 tgcccgcaag ttcgacttca ccggtggcca gatcaggatt aagagtttcc gcggggcgcc
    11461 ttgacaggac accgcgcacg gcaatcacat cttccgagcg cagctgtttg gccttttcgt
    11521 gggcgcagag atccacttcc ggattaaaaa cgatttgcgt gatgccgctt cgatcccgta
    11581 aatcgacgaa gatgagcccg ccatgatcac gtcgcgtatg cacccagccg gtcaggacca
    11641 actcgcgtcc gaccgctccg gcgcgcggct ctccgcaata gcaactccgc ttccaatcac
    11701 cgagctgatc gcaaaaaact ctctcgattg ccatttagtt agccttcctc gacaacaacg
    11761 cttcctcaac atcgttcaaa cctatctcac tctgctgcct cgttgccatg tcgcggagca
    11821 aagctactcc tttttcaagt tcattatcgc cgatgatgag aaccgatgat gcattgagct
    11881 tatcggcgcg tctcatttgg cttttgaggc ttctaacctc gccctctaac tcaatggata
    11941 acccgttctg gcgcagccgg tgcaccacag gaaaggccca ctgctgagcg ttctcaccga
    12001 cccaaactat atagaccgcc ggtccggaaa gagccgagct tgtcttggaa cgcagtaaca
    12061 tggtcaagcg ctcgacaccg attgcaaagc ccacgcctgg agtcggcggg ccgccgagtt
    12121 cctgaaccaa gccatcgtag cggccgccgg ctgcgatggc gctctgcgag cctaattggt
    12181 tacttgtcca ctcgaaggta gtgcggcaat aataatccag gccacggacc atgcgcgggt
    12241 tcaaatcata agccatgccc gattgacgca gcaggcgctg aactgtttca aaatgtcgac
    12301 ggcacgggtc acacaacgac tcgagaattg agggagcata cttggtctgc tcaatacagc
    12361 ctggctcctt gcaatccaac actcgaagcg gattccgttc catccggcgc cgacaattag
    12421 cacaaagcgc ttcctcacgc tccgtcagaa atgccaagag ccgctgtcgg tacttcggac
    12481 ggcattctga acaacccagc gaattgagct gcaatgcagc gccttctagc ccgatggcag
    12541 aaaaataatc gtttaaaagt agtaggactt cggcatctat aaacggatcg ctccgaccca
    12601 atacttcggc gccaatctga tggaattgac gcatccggcc tttttgcggc cgctcgcggc
    12661 gaaacatcgg tccgagataa taaagtttct gcacaggctc agtcttgaac aactccgatt
    12721 ccacataggc tcgcaccacg cccgcagtgc cctcagggcg taatgtcaaa agcgaactct
    12781 ttgcgtcctg atcggcgaac gtatacatct ctttttccac tatatcggtc gtttcgccga
    12841 gtgaccggtt aaagagctct gttttttcca caatcggtat tcggatttcg gcaaagttat
    12901 acgccgtgaa aatcttgcgt gcctcttttt cgacccgctg ccagaactca acttcatcgg
    12961 gcagtatatc cgaaaaccct ttgattgcag tgactttcat tgagctttat cgatcctttt
    13021 cgagatccaa aacctgatcg gccttgcaga tgccccgagt catgactcgc aagtcatggc
    13081 gagtcgagct gcagacaatg acttcgctgg tttaacgtaa gggcaatgtg gaggaacccg
    13141 aaaactcggt tacatttaac aacataagaa attatgcagg tcaagcaacg ctgattctca
    13201 aaattggaac ctgttgcgaa gccgattctc cgttatcctt gcatcttgta tcgataggcg
    13261 cgactacggc gcgtgctgcc gtgaagctcg gcaaaaagaa accctcgaaa gatcataaaa
    13321 tagctgagac agagacccat gccgactgcc acaatgaaaa tttgcacgac gccgggtagt
    13381 ccaatcgaaa tagcgctcaa gatattaaag atgcaatagc cggctagggt cagaacgatg
    13441 ttcggcagaa accactctag ccaattctcg acgacaaaat tataactatc gctaatcacc
    13501 ccaattccgg aagaacgggt ctgataaata agctcaggag ccgggttcaa cagaatatag
    13561 actgcaactt gaattagtgc gaagagcagg ccgccgttgg gggtcgatgc gagcgccatc
    13621 gccaagaccc gcattggaat ccacaatata aaagcgattc caagtaattc ccatagataa
    13681 actgtaaagc cattgatgaa gtccttgaaa tctgcgcgtc ggccctcgac tatattctta
    13741 accaagtata acgctgagct aatacatgcc tgagtcgcca aacctaaaac caaaccgccg
    13801 atcatgccta aaggcgcgac catgagtgaa acgatcgaca gcagcgccgc ataggcccaa
    13861 ggggcaagac tcacgaccca gttcttgagc aggctttccc atgagcggcg caaggccgtc
    13921 gcgtaaagcc atagagtcgc ttgtaccgtg ctcattgaca tccttatacc agataacaga
    13981 aaaaattctg ctgggcggcg aactacgtcc ggacaatcat tggctggtta ttatcctctt
    14041 ttcgcttttt taagtcgaag ttaaacaagt cgcctcttct aaaacaccag ccggagaatg
    14101 aaagattcat tgcttcatta tacagacgtg tagtcatttc ctagcaaacc agagcagcct
    14161 gatcctacag ccgtacagaa ttgggttgtt actctagccg ccaaatacac aatatccccg
    14221 acttctgatc cgccaaccca caagccagac cgattgtatt gagtgttttc aacgggttag
    14281 ctagagcctg gcagatctga tcaacgatgg cgaatttttt gcagccgaac ttcgatcact
    14341 gccgcgaaag cgtccccttg aactggcaaa attctaacaa ggagaatctc ggatgagttg
    14401 cacaaaagct agaggttatt tcctcgaggc aactctcagt tgcttgagga gccggcgaaa
    14461 tctgcagtgt ccactctaaa aaatattacg gcgcatttca taacgattga tccgaaatct
    14521 ctgccacgga cagaaacttc tggcgctaag atctgttcgt tagttcaaag cagttcgcta
    14581 agcaaaaaat gatttacata gtcacctacg acgaaacgcg atatcaccgc caaaacgtca
    14641 agcggctgct tttggtggaa tcgaatttgg gtttggaact cgaacgcgtc accggcctgg
    14701 tgagaatgct aaccgacggt aacttcgccg cggctagtgt agagatcgcc ggacagagag
    14761 actgggacgc tagacgcgtg cgacatcccg ccatccgttc ttacaagctg gattgattcg
    14821 ccggcggacg ccgcatattg gctgattgtc gcctcttggt cagaggtgat cgggccgcat
    14881 aatttccttc cagtttctaa ccgcttagaa aagataggtt tcaattccaa ccagaagcaa
    14941 cgtcaaactg atagccaata tcttctcttg agatcgaaag cgcatcccga aagaacattc
    15001 caagagttga gtctcactaa gctctccggt gcccaacatt gccgcgagaa agttcaagac
    15061 actggctgaa gatgcacccg ttggcgatgc tatcccagct gtgcttcgcc aattaggttg
    15121 tttgccgaac ctaggttaag acggatatgg tctctgcgag ataatctcga cggcggtata
    15181 ttcatcgggc aaatttagtg gtagctttac cgtgccagag gaagacatga gcgccaacga
    15241 gattttaggc ccgactccgc tgctttcttc tcgtaatgat aatgctgggt ctaggctcgc
    15301 ttgcatcgcg cggcgtagct cgcgtatgcg ccgatgttcc cattcgggtc ctatcttgac
    15361 ggatcaaatg tagtagcttc cctgtgataa aggtaaatcc aacttgaaag cgatcagcac
    15421 aaatgcatac acggattaaa ataacctttc ttgcctcact agtcgtgatg ttgctttgga
    15481 gagctctgcc aatccaggct ggggcgccca ccgaagagat tcgcacagct atcgatcaag
    15541 gcgttcagat ccttaaatcc gccaaactcg ataatagcaa gcagcgagct caagtcatcg
    15601 atcaattgcg cgaaatcgtt tacacccgat tcgatttcga ggaaatggcc aagcgatctc
    15661 tcggttctca ctggcggcga cttaacccta cacagcaaaa agaattcgtc accgcgttca
    15721 cggacctttt agaaacgact tatgccgaca agatcgatct gtatgagggg cagcaggtcg
    15781 aatataccgg tgagaccata gacaagaact acgccgaggt gaacacgaaa gtcatcggca
    15841 agaatcggga aagctattcc gtcgattacc gcctccacca agcgggcggc aagtggagaa
    15901 tatatgatgt ggtcgcggag aacatcagct tagtaaataa ctatcgatca caattcaacc
    15961 gaatcgtggt caattcatcc tttgaagaac tcataaagag gatcaaacag aaatccagct
    16021 agcttaggtg agtgaggcgc aactttacta ttctaaccgc cgcgcggcat cacgcagcga
    16081 cgtcatacag ggccaatctg tagtatcgcg attcccgcta agtttcggaa tgctcaactc
    16141 gggcagagca aaagatcgcc gatacggggt ttaaagccct gtggataacc cgtaaccgcc
    16201 aggttagggg attattggcc aatcctgcca aaaattgcca aaaatggtaa aaatcgccgc
    16261 atcataagac ttcgttaagg acacggtttc aaccctaacc cccataaaat atgagattta
    16321 tggtgcccac ttaataggca attcgctcga cagcagtact gtacggcggg tcgctgacgg
    16381 cgaatgtgac ttatcaacaa agttatccac tgcttttgtg aacatcagat tgttgtcgat
    16441 tgtcaccgga tcgtggagat gacgcagcct tcggaacctg tgactgcatt tctagtccaa
    16501 ggagatcgca tcgatggctg actatcaggc gtcgccctgt ccatcagctt gcaatctttc
    16561 ggcgcatcat tttgcgagag cataagttta cagaacgcat cccggatcga taaacttcca
    16621 gacaagtaag cgcattagcc aagttgattt tttcaaaaag tctcagctcg ttctgcagat
    16681 gtttgctcag cggtgtcgga aaagaaacca accattttcc aaactatttc gcgtgtttgg
    16741 acaggagaat tcgcggcaca acctttccct ccacgaccca acaccgtctt ccatcgaatg
    16801 accttggatt ttcaaaacgc gtggccgctt tccccaatca tccttctcca tgtattcttc
    16861 gaataagtag tgcggctcat gaaaatgccc atccgaatcg ataatgcgca taaatgtatc
    16921 tccccattct gcaaaatact ggttgatcga cagattatgg cgaaagattt ttaccatcca
    16981 gtcgctgtga tgccggcggc gcaatctaaa aaagaatcgt cttcagcttg gctaagatgt
    17041 cagcgtccag cttgaagatt ccatccaccg tagatttcat gtcttccgct gttaccggcg
    17101 ccaatccgag ctttgccttc tctgcttccg cgataaattc tttgtctttg accgtacctt
    17161 gcagagcctt ttgcaggatc tgcgcgcgat cggtaggcgt gccgggaggc aacgcgagag
    17221 ttttggtgat cttgcttgga taatggatgc cgacttcaat cagttgtttc gcttccgggg
    17281 tcttggcgag atcgatggcc aagggcacgc gcggcagatc cggaaatggt ttggggacag
    17341 tctgcaacac gattaccacc gtgcctgcct cgattgcctt ctgccatgct ccacgcatgg
    17401 tgtcccagcc ccaggtcgtc ccatcgatct cgccgctttc ggcagcgagc cgcatctccg
    17461 ccgtcccctt gtatccggag atgatctgga tcggcaagtt caacgcggcc ttgatgactt
    17521 tgatcacgtt gtcggtgccg aaggcgcccg gtccggtggt gcccatcttc accggcgtct
    17581 tggccgccat ccattgttcc atattgttga tgccgctcgc tttggttaag gcgatggcgc
    17641 tttcgtctcg cgcgaccgcg ccgacaaaaa cgaacttgcg cgcgtcgaat tcgatcccag
    17701 gctgtcccat gacttgattg aacgcgaagc cgccgctgaa gtgaccgaac gtcaaaccat
    17761 cgggcttggc gacattaaaa atgtgattgg cagcgagcag gcttccggcg ccggtcatat
    17821 tatcgacgac aatggtggga tggccaggaa tatacttagg catgtgccgc gaaagcatcc
    17881 gcgccagagc atcgtaaccg ccgccggccg aaaaaccgac gatgatacga atcgttttcc
    17941 cctcatagaa ctgattctgg gccgatacag gcctgctaaa aaagcagatg caagcgatta
    18001 tcgctgtgtt cattatcagc ttcttcatga acggcctcct tgcagcctcg acgtgcaaac
    18061 ctaatgaaga tgccactaga acgtcaagcg gaaaacatgg aatcataaag ttgacggttg
    18121 atccgccaca agctacattg ccagtcacat ttcgatcact cctgttccat tccccgtgac
    18181 aaaatactca gaaacaaaca gcgccagaga ccccgcggca attatccgac cctggtcttc
    18241 tctcctgatc cgagactatc gcctgatttt catcgcaatc ctgtgcgcca acacttcgat
    18301 gcacatgcgc aatgtggcga gcttatatca tgtctaccat ttgtcgggat cgtcggtgca
    18361 gcttggtttg gcgggatttt ttcaggccct accgtttata ttctttgggt tattcggtgg
    18421 cgtgcttgcc gacacttaca atcgaaaaaa actgatccta ttcaccgccg cattcaatct
    18481 gctgcccgga ttggctctcg gactcttgac caccaccggc gtaattcagg tctggcatat
    18541 caataccttg aacgtggtga catcatccct gcaagtactc ggcggtccat cgcgccaggc
    18601 gattataccg agcctggtgc cgcaatcgta tcttctaaac gcagtgactc tgtcgacctt
    18661 gatgatgcag gggacgcagc taacagcgcc ggtttttgcc gggttcatga tcgatttttt
    18721 cggcgtggat gcttcctata ttgcggatgc tttattgctg atgccggcga tttgctgtgt
    18781 tttgctgata cgtagttcag ggcaaccgca tggcgcgcgc cgacaggtaa gctggcgcag
    18841 cctgattgag ggattcgagt tcctctatca tacgcgtatc atcctatcgt tgtttctttt
    18901 ggacttcttc gccgtgctgt tcgggtttta tcgtccgata ctgccgatat ttgcagacga
    18961 cgtctaccaa gtcggcgctc gaggactcgg cgcgctttat gcggcgccgg caatcggagc
    19021 tctttttggc tcgggcatgt tgctcgctct tggggacatc aagcgcaaag gagccctgtc
    19081 gattgcggca acactgctct ttgccctgag cttggggttc ttgggattgt cgagatggtt
    19141 ttggctcggc ttgatagcgg tcggcgtttt gggattggcc gacgccatca gtgtaaccat
    19201 gcgtcgcacg gttgtgcaga tactcgctcc cgacgatatg cgcgggcgcg ccaccagctt
    19261 tttaacggtg tttgcccaaa ccaccaacgc ccttggagca gtcattgcgg gcggcggagc
    19321 ggcgctgctc ggcgcgccca acgccgcgct ggtgggctgc atactttgca cgctcaccgt
    19381 gctggctgtt tgctgggcga ttccccaatt gtggtcgtat cgctcggata cgcattgagt
    19441 cgcaagagtt caaacgttca acagttcaag cttgacgtag aacccgctca ctcgctcacc
    19501 ggggttgaat ggatgcattt ttttaaccgc ctgctagtgt atgatgtgat tgtcggtgcg
    19561 tcgtagagat gaattccaac cgcattagtt cgcaggaaaa agccttacac aatttacaaa
    19621 gtgtcctcga tcccgtgcgg gcccttgccc cgagcgccga tctgagcagt ttggaacgcg
    19681 cccgatcgct ggtcgccagc aaattctcgg agaacgaaca ggcgcgggca tctatcaagc
    19741 gcggcgccga agccgttgag cgcttgggta aactcggaat ggatgccgag acactggcag
    19801 ccgccctgct gcttccttta gtcgaagagg gcgtgctcga tccggcgatg acagagaaaa
    19861 gcattggcgc tgaggttgcc cgcctgaccc aaggggctct tcgtctcgcg agccttaaag
    19921 atctgcgcaa aaccgcaggc gagagtttac aggcgaatcg gctgcggcag atgcttttga
    19981 cgatcgccga ggagccacgg gttgtgcttg tcagcctggc acaccagctt tgtgctctgc
    20041 gcgcagccag ccatgctccg gaacagatac gacgcgccct tgctcaggat agcctcgagg
    20101 tgttcgcgcc gctcgcgaac cggttgggcg tgtgggagct caaacaggag ctcgaagatc
    20161 tcgcttttcg atacttggag ccggacacct ttcgacgtat ctctgacggc ctcgaccaga
    20221 ggcacacgga tcgcgagcgt tacatcgcgc aggttgttgc gcgattgcgc gaagaattat
    20281 ttcgggctgg gatcaacgcc gagatcagcg gtcgccccaa gcatgtttac ggcgtgtgga
    20341 acaagatgcg ccagaagggg ctcgagatca gtgatgtctt cgatgtcctc gcttttcgga
    20401 tggtgctcaa tgacgtgagc gactgttatg ccgcactggg cgtggtgcac gggttgtggg
    20461 agcccatcct ggatgcgttt gacgactaca ttgccaaacc caaagccaat ggctatcagt
    20521 ccttgcactc ggccgtgatc ggcccggagg gagtaaccat cgaggtgcaa atccggaccg
    20581 tcgccatgca ccgtcacgcc gatctggggg tggcggccca ttggcagtac aaagaaggcc
    20641 tgccaaggcg catcgcgtgg tcgcgcggat tcctggaagc cgcgcagagc acgtccgatg
    20701 gcgtagacat tatcgaccgt ttcaagcaag cggcttttca cgatcgaatc tatgtgctca
    20761 cgccgcaggg tcatatagta gatctcccgg ctggcgccac gccgcttgac tttgcctacg
    20821 caattcacac ccaagtgggc catcgctgcc gcggcgccaa ggtcgatggg gtcattgtct
    20881 cgcttacaac gcctctcgca agcgggcagc aggtcgagat cctgacggcg cgggcaggcc
    20941 ggcctagccg cgattggcta aaccccaacc ttggatattt aaaaaccgcc tctgcgcgct
    21001 caaaggtccg tcgctggctc acagagcagg atcatgaagc acatgtagcc cagggtcgga
    21061 cggtgctgga gcgtgagcac cggcggttgg gcctcagcga gattaatatt gccgaactca
    21121 ccaaacgctt tgaaatgcgg cggcccgaag acctgctggc ggcgctgggc cgcggcgaca
    21181 tcagcattgt gcgactcggc tatgcgcttg gcattcaggt tcctccgcca gtgccgagta
    21241 ctccggcatc atcgccgcaa ccgtcctcgc gggccagcct actggtccac ggtgccagca
    21301 acgttttgac gaagatcgca ggttgttgca gacccgtgcc aggcgaaccc attggcggct
    21361 acgtcaccgc gcagggaggc ggcatctcga ttcaccagcg cgattgtcgg aatctcaagc
    21421 agcttgcgga gctacgacct cacaagttgg tcgaagttgc ttggggcgaa ataagcgggc
    21481 agcgtcctgc cgtcgcggga atgccgcgga agccagtctc ggattgaaag caagtaggta
    21541 attaaaaacc tgaccatact aatctgctgg tgaaggctag gcgtgattgc gcgagcatgc
    21601 agctactcgc taagggaacg gccaggaggc caatccgcgc aaagaccgga ctcggagcca
    21661 ttccgatggg tccgagttcc gacggagacg gcagaaacat ttactttacg ggaccggatc
    21721 tctcgtccca ggaccccatg atcccgggcg agacgtttcg atacatcggc attcggctga
    21781 ataaaaatgc gcgccgcgcg gggcaccgtt ttcgcagctg ccaagcacgc gagagcgcca
    21841 gcgccgaccg aaggccgaat tttcttcctc gataaacgac cattacgcca acgccatttc
    21901 gttggccttc ttttgcgtcg gcgcaaagga cgacgcaaac ttcacgtaga gcacccttcg
    21961 cgcaacaagc attcagagcg
//
LOCUS       DASUVS010000358         2047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_150242, whole genome shotgun sequence.
ACCESSION   DASUVS010000358 DASUVS010000000
VERSION     DASUVS010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150242"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VF182_19120"
     CDS             complement(<1..258)
                     /locus_tag="VF182_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439239.1"
                     /translation="MAIIDLDSHFRDGWLLDEIYRLPEPFARHSPKRIGEGKYFYSKF
                     EHDLSPMEDPVANANFKKPVTHQVFYNPEANQRGNEVMRWQQ"
     gene            628..906
                     /locus_tag="VF182_19125"
     CDS             628..906
                     /locus_tag="VF182_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439240.1"
                     /translation="MKTLTITDAKKNLGRWLAAAARGEDIGIVCGSDIVALRKVEVES
                     TDYAQREYGAPPEQVAALDKATEQRYRRLKRGKKLTSVTTGELRKMLE"
     gene            899..1189
                     /locus_tag="VF182_19130"
     CDS             899..1189
                     /locus_tag="VF182_19130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439241.1"
                     /translation="MSKLLAIDPAVIRRLRELPKNERVECLLALCDLVDGFGHPHVHS
                     GLGIRKLGGKLFECRGNRDLRFIFQDRGAELFVSFLGNHDEIRTLLRQGSYR"
     gene            1684..1818
                     /locus_tag="VF182_19135"
     CDS             1684..1818
                     /locus_tag="VF182_19135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439242.1"
                     /translation="MINVGIPAKGIPEITPVVLLNERHSHFLSKPDEAHRLIREFVAE
                     "
BASE COUNT          473 a          549 c          567 g          458 t
ORIGIN      
        1 ctgctgccag cgcatgacct cgttgccgcg ctggttcgct tccgggttgt aaaacacctg
       61 gtgggtcacc ggttttttga aattcgcgtt ggctaccggg tcctccatgg gagagaggtc
      121 gtgctcgaac tttgaataaa aatatttgcc ctcgccgatg cgtttgggag agtggcgcgc
      181 aaacggttcg ggcagccggt aaatctcgtc gagcagccag ccgtcgcgga agtgagaatc
      241 taaatcgata atcgccatga aaactcctct ttggcacaat cagtatgcgc ccggcaagaa
      301 agtcgccggc gacaatgcgc tacgctccga agctagtaca gcatcgctcg tccgtcaaca
      361 tatcgaacga tggacgaggc atctgttctt gaagaatcga acctttgaac tggttggcca
      421 gctaagcctt cgaaccattg gaacgggctc gatacggggc cgagcgaagc tattaagcat
      481 tcgagaacga cgaacaatta gatgtcaata agttttatgg ctgcgcccgc tcctaactgt
      541 ctcgctttga actgtggcct ggccttgcat ctcgaaccat gattcggaag tattgacaag
      601 ctcttttatc gactatataa agtctttatg aagactttaa cgattaccga tgctaagaag
      661 aatctcgggc gctggctcgc cgctgcggcg cggggcgaag atatcggcat cgtttgcggt
      721 tccgatatcg tcgcgctcag gaaggtcgag gtcgagtcca ccgattacgc tcagcgggag
      781 tatggcgctc cgcccgagca ggtcgccgcc ctggacaaag ccaccgagca gcgctatcga
      841 cgcttgaaac gaggcaaaaa gcttacttcg gtaaccactg gagagcttag gaagatgctt
      901 gagtaagctt cttgccatcg accccgccgt tatccgccgc ctgcgcgagt taccaaagaa
      961 cgaaagggta gagtgcctgc tcgctctttg tgatttagtg gacggctttg gccatcccca
     1021 cgtgcacagc ggcttgggga tccgcaaatt gggcggcaag ctcttcgaat gccgcggcaa
     1081 ccgcgatctg cgatttatct ttcaagaccg aggcgcggag ctttttgttt cctttctcgg
     1141 caaccatgac gagatcagaa ccctgcttcg tcaaggcagc tatcgttgat cacttaagtc
     1201 gcgagtcgtt taagatcctg gtgcaaaagc tagactactg agcgttgaaa ttctctccag
     1261 gcattgagat tgttaacgac tggagcagag cgtagtcatg gaactaccgg cgcgattgaa
     1321 acggcttgat ccggtgacgg tgttcacgcg gccagccgga tttgactaat ctatcacttc
     1381 ggcgtttcgg gaattctcga aacgtggaag ttacccgccg atataactca cacactatcc
     1441 ggattgcgcc cggcccggca cgcggcgctg cctggttcgg gtaagctcag gtctctaggg
     1501 tcaactaaaa aacacgaatc cagcctccgg ccggcatcgc cgaattcgta atgcgtgtca
     1561 ggcggaattc gctacgggtt catcacttcc aggtgatgaa acttccctgc gcggtcgttc
     1621 ccccgagggg agatcgccca agtgggtagt tgcaccgaga acaagtcaat tgacgctgac
     1681 cccatgatca acgtcggaat tcctgcgaag gggatccccg aaatcacgcc ggtcgtattg
     1741 ctcaacgagc ggcatagcca tttcttgagc aaaccggacg aggcgcaccg tttaatccgc
     1801 gagttcgtcg cggaatgaag gcatcacgcg gaaacatgaa attggatcag acgagatccc
     1861 gattttctcg atcggggagt gtgcactgag tcgcgtgttc gcgtcacctg ttcgtggcga
     1921 taaccctcat gaatcagaca gacgatccag cgtttccgta ttcatcctgc cgatttataa
     1981 gacagagcta cacggagcag aacgtgagaa agtgagccgc ttcaagcgcc gacccggacg
     2041 tagacgc
//
LOCUS       DASUVS010000359         6626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_588396, whole genome shotgun sequence.
ACCESSION   DASUVS010000359 DASUVS010000000
VERSION     DASUVS010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6626
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588396"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VF182_19140"
     CDS             complement(<1..674)
                     /locus_tag="VF182_19140"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6439243.1"
                     /translation="MSSLRLNRLFVIALMTIVFGGCRIEDKFIFHPDRVSARDPSHVG
                     LAFENILFTAQDGIRLHGWFIPHPEARATLVWYHGNAGNISDRLLNIRLLHDRIKINI
                     FIFDYRGYGQSEGSASEKGTYLDGEAAIKYLLGRDDAAARGLILFGRSLGAAVAAEMA
                     IRFTSLGLILESPFVSIREMAQAIFPSLPIAWLLRTRYDTLEKIRRVKTPIMVLHGDH
                     DATVPFA"
     gene            complement(700..1218)
                     /locus_tag="VF182_19145"
     CDS             complement(700..1218)
                     /locus_tag="VF182_19145"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEX6439244.1"
                     /translation="MARDLKEAFFRLDGAWASFELGSIRRRDDYLKPFRVLRCKIDDQ
                     VDFQTPGAPRSSTEASVLIEIFGEQELVAATGRGPVHAVDNAMRKILEKHFPQLAEVK
                     LEEFDVRLLPYEPPQEDDQNGAGALVRVLGIFSDGEDRWGTVGVAVDILQASVECIID
                     GLEWKLRGEQKH"
     gene            1444..2013
                     /locus_tag="VF182_19150"
     CDS             1444..2013
                     /locus_tag="VF182_19150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439245.1"
                     /translation="MEKQSLDNKLRDFFLNAVRQSFWQLGIYDATVAGYVADVLTDFA
                     RTDSLYRLRSRAGRKIESVVEMLAHESEPPANENQLLRERETRKYVGDYALFMSGIFR
                     TYVEGRGFLDYYIKEGSRSYWTVSELDVSLYRTGFILFQELSTKFEYYSGALDYTRKA
                     YFAAQPGEDPFAGFLRQVDGWMKVNLTEN"
     gene            2068..3099
                     /gene="mltG"
                     /locus_tag="VF182_19155"
     CDS             2068..3099
                     /gene="mltG"
                     /locus_tag="VF182_19155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX6439246.1"
                     /translation="MKWFLFTVLALIVTGLLFGGFLVAHIFTASSGNAKPVEVRVEQG
                     EPFSAVVRKLRAHRVIPNDKLFSLWARLTGSDKKIQWGVYRFDPALAPQEVLHRLVTG
                     KRVFLMVTIPEGLTIKEIAELLAKMQIADRDKFLAAAADPELLATLGLADKGLEGYLF
                     PNTYQFTPMTSEKEIIRTMAEQFRKASEPIWAQLGSDYKLSRHEIITLASIIEKETGI
                     EAERPLVSAVFHNRLKRNMALQSDPTAIYGLKQFDGNLTRKDLKDQNPYNTYRIPALP
                     PGPICNPSLASIKAALQPADVPYLFFVSRNDGTHVFSQDLESHNQAVRTHQPVREVPP
                     FQRKTSGLH"
     gene            3304..4512
                     /locus_tag="VF182_19160"
     CDS             3304..4512
                     /locus_tag="VF182_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439247.1"
                     /translation="MPALPLLVLVSAIIVIGVLAVVFRFRRRVPSIPAAARLMDDKAQ
                     AQDRFITLTTVPSSPASDPMLARLRWEAMGTLSRVELRRDFPYRLKQPFYWSAISSLA
                     LIILFHLLLNGTLTKSSVSPQQRIRQLAEQMSQRVPLAEIGRSLQSLADKLDDLKVAS
                     EEKQSLIEEIQKQIAQEQETEQEQNNKDLLSDASSTLKSLEQESGGPGQAEQQNAGGG
                     VQSNPSQEGQGEGKESEGGGDSKSEVSAQLNPDMKDGKSAQGNSQGKGQEKDSGHQKE
                     QKANQPDPSNTEKTTEESAGKMKENAEGRSGKNRSDEMPKDGPPAERYRQPGEQGPDS
                     IKGAKYVTVQLPEEIASGTSGVTVSSKDGKESKNRPRVPISNVPLPAHIPEAPAEKQR
                     MPLEYRDLIR"
     gene            4530..5510
                     /locus_tag="VF182_19165"
     CDS             4530..5510
                     /locus_tag="VF182_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6439248.1"
                     /translation="MDPEKFQESFKRLEAEVRKVIVGHDDTIRKVLIAFFAGGHVLLE
                     GVPGLGKTLLVKTLSHALGVSFKRIQFTPDLMPSDIVGTQVLAEINGRREFQFKQGPI
                     FAHVVLADEINRATPKTQSAVLEAMEEKQVTVFGESHALESPFMVLATQNPIELEGTY
                     PLPEAQLDRFFFKLLVMPPTPVELREILKRTTGTETADMQEVLPEDEGKLINEMKQLL
                     RQVLIAPPIEDYVVRLVHATQPQPQNAANSPVRQYLRFGSSPRGAQSVILGAKGHALA
                     DGRVHVSYDDVENVLYPALRHRMILNFQAEAENVTADQIMDEVMKQVGRN"
     gene            complement(5579..6487)
                     /locus_tag="VF182_19170"
     CDS             complement(5579..6487)
                     /locus_tag="VF182_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX6439249.1"
                     /translation="MQRVLRMRRTRRVLLFLTVGFFLTSATLYAQADKELIAKGQYLF
                     AIAGGCACHTVPKETYHTGGRAFPIPFGTVFSTNITQDKETGLGDWSERQIYDAMNAG
                     RRRDGSRLVPVMPYEAYSGMAPEDLKALIAYMRTLKPVKKATPNLKTWVPLVRSVGVP
                     VFLNLFGGSSNSPAQAPKSGVERGRYLVRHVSICGDCHTPRNSIGVQKSSLYLAGAGA
                     KDGPLGEKVPNITPDKETGIGDWKREDIAELLITGTKPDFDNVQGLMHEVIQGTPHGY
                     KDMRREDALAIADYLKSIPPIKNKIE"
BASE COUNT         1594 a         1729 c         1791 g         1512 t
ORIGIN      
        1 gcgaagggaa cggttgcatc atggtcgccg tgcaaaacca ttatcggggt tttaacccta
       61 cgaatttttt cgagagtgtc gtagcgggtt cgaagcagcc aagcaatcgg caacgaagga
      121 aaaatcgcct gcgccatttc ccggattgag acgaatgggc tttccagaat aagtcccagg
      181 ctagtgaatc ggatggccat ctcggcggcg accgccgcgc caagcgatcg accgaacagg
      241 attagcccgc gcgcggcggc atcatctcgc cccagcagat atttaattgc cgcctccccg
      301 tcgagatacg tgcctttctc agaagcgctc ccttcgctct ggccataacc gcgataatca
      361 aaaatgaaga tattaatctt gatccggtcg tggagcagcc tgatattcag caggcgatca
      421 ctgatgttgc cggcgttgcc gtgataccaa acaagagtgg cacgggcctc tggatgcggg
      481 atgaaccatc catgtaagcg gatgccgtct tgcgcggtga aaagaatgtt ttcaaatgcc
      541 agtccgacat gactcggatc gcgagcacta acacggtcag gatggaaaat gaatttatcc
      601 tcgatcctgc aaccgccgaa gactatagtc attagcgcta tgacaaacag acgattcagt
      661 cgcaatgaag acatggacgt cgccgacact ggcgcagctt caatgtttct gctcgcctct
      721 aagtttccat tctagtccgt cgatgatgca ttcgacgctg gcctggagga tatcgaccgc
      781 gacgccgaca gttccccaac gatcttctcc atcggaaaag atgcctagaa cacgaaccag
      841 tgcgccggcg ccattttgat cgtcttcttg gggcggctcg tatggcagca gccgaacatc
      901 gaattcctcc agcttgactt cggcgagttg cgggaaatgt ttctcaagga ttttgcgcat
      961 ggcgttatcg actgcgtgaa cagggccgcg cccggttgcg gcgaccagct cctgttcgcc
     1021 gaaaatttcg atcaaaacgg aagcctccgt tgagctccgg ggtgctcccg gggtttggaa
     1081 gtcgacctga tcgtctattt tacagcggag cactcgaaaa ggtttgagat aatcatcacg
     1141 gcggcgaatc gatccgagct caaaggaagc ccaagcgccg tccagacgaa aaaaagcctc
     1201 ttttaaatcc cgcgccatcg ctaattcctc ctttgaaaat ggcttgatgg gccattgtgc
     1261 tgtctactta cacagaagcc caaaaattaa cattggtcaa tttccagcta gtcgcgctga
     1321 tcagccgtgg tttatcgttt gatcattatc agaatgtcgg cgcgcgtacg taacaaggac
     1381 gctgctggtc cctatgatat tcttttcaag cccgggtaag tttgctatca tcagtcactg
     1441 gaaatggaaa agcaaagctt ggacaacaag ctccgggact tcttcctgaa tgcggttcgc
     1501 cagagtttct ggcaactcgg catctatgac gcaaccgtcg ccggctacgt cgctgacgtg
     1561 ttgaccgatt ttgctcgcac cgatagtctg tatcgcctcc gttcgcgggc aggccgaaag
     1621 attgaaagtg ttgtcgagat gctggcgcat gaatccgagc ccccagccaa tgaaaaccaa
     1681 cttcttcgtg agcgagaaac ccggaaatac gtcggcgatt atgcgctctt catgagcggt
     1741 atttttcgaa cttatgtgga aggccgtggt ttcctcgatt attacatcaa agaaggtagt
     1801 cgctcctact ggactgtttc ggagttagac gtgtcgctct accgcaccgg tttcattctg
     1861 ttccaagaac tttcaaccaa atttgaatat tactccggcg cgctggatta cacgcggaag
     1921 gcctattttg ccgcgcaacc cggagaggat ccctttgctg gttttctgcg gcaagtggac
     1981 ggttggatga aggtcaacct tactgaaaat taattggaac gcctgccatc tgagccagtc
     2041 tcgtcatctt taaaagatta acgaatcgtg aagtggtttc tttttacggt acttgctttg
     2101 atcgttactg gattgctgtt cggcgggttc ctggtagcgc atatttttac cgccagctcg
     2161 ggcaacgcca agccagtcga agtgcgagtt gagcaagggg agccattttc ggccgtggtc
     2221 cgcaaactgc gggctcaccg agtgattcca aatgacaagc tattctcatt atgggcgcgg
     2281 ttaaccggtt cggataagaa gatccaatgg ggtgtttatc gcttcgatcc tgccttggcg
     2341 ccgcaggagg ttctgcatcg cttggttacc ggcaagcgtg tctttctaat ggtgacgatt
     2401 ccagaagggt tgacgatcaa agaaatcgcg gagctgctcg cgaaaatgca gatcgccgat
     2461 cgtgataagt ttcttgcggc cgctgcggac ccggagttat tggcaaccct tggattagct
     2521 gacaaggggc tggagggcta cttatttccg aacacatatc agttcacccc gatgacttcg
     2581 gaaaaggaaa tcatccgcac catggcggag cagttccgca aagcgtctga acccatttgg
     2641 gcccaactcg gtagcgatta caaattgagc cgccacgaga tcatcactct agcatcgatc
     2701 attgaaaaag aaaccggcat tgaagccgag cggcctttgg tttcggcggt ctttcacaat
     2761 cggctcaagc ggaatatggc attgcaaagc gacccgactg cgatttatgg cttgaaacag
     2821 tttgacggca atttaacccg caaagatctt aaagatcaaa atccttacaa cacctatcgg
     2881 attccggcac tgccacctgg accgatttgc aacccgagcc ttgcctccat caaagccgct
     2941 ctacaaccgg ctgatgtgcc ctacctgttc tttgtttcga ggaacgacgg cacccatgta
     3001 ttctcccaag atttggagtc gcataaccag gcggtgcgga cccatcaacc ggttcgggaa
     3061 gtcccgccct tccagcgcaa aacgtctggc ctgcattagg cgcgtttatg ccatccggtc
     3121 gaagtgttat agtcgacagt tatgcaaggg tatctaggta accaggatca tggatcgccg
     3181 gaggattctg ttcatcgcct agttagacac ttgcgtaatc aggtcgttcg ggatctcctg
     3241 cttacttttt ctccgccgat tttgactgtc atctatttag ccgtcttgta tccgggatca
     3301 tggatgccgg ctcttccgct cctcgtgctg gtatcggcga taatcgtcat cggggtgctc
     3361 gcggttgttt ttcgatttcg acggcgagtt ccttcaatcc cggcggcagc gcgtctgatg
     3421 gatgataagg cacaggcgca ggatcgtttt ataaccctga ccacggtacc atcatcgccc
     3481 gcgtccgatc ctatgctggc tcgccttcgg tgggaagcca tgggaacgtt gagccgagtg
     3541 gaattaagac gcgattttcc ttatcgccta aagcagcctt tctactggtc ggcgatcagt
     3601 tcgctagcac tcatcattct gtttcatttg cttttgaacg gcaccctgac gaaatcgagc
     3661 gtttcgccgc agcagcgaat tcggcaatta gcagagcaaa tgagtcaacg tgtgccattg
     3721 gctgaaatcg gccgtagcct gcagtcccta gccgataagc tggatgactt gaaagttgca
     3781 tcagaggaaa agcagtcgct cattgaggaa atccagaagc aaatcgcgca agagcaagag
     3841 acggaacagg agcagaacaa taaagatctc ctaagtgacg ccagcagcac gttgaagagt
     3901 cttgaacaag agtcaggcgg tcccggccaa gcggaacagc aaaatgcggg tggcggcgtg
     3961 cagagcaacc cttcgcaaga gggtcaaggg gagggaaaag agagcgaagg cggcggcgat
     4021 tctaagagtg aagtgtcggc ccaattaaac ccggatatga aagacggaaa atcggcgcag
     4081 ggaaattcgc aaggcaaggg acaagagaaa gactctggcc atcaaaaaga gcagaaggcg
     4141 aatcaaccgg accccagcaa tacggagaag acgaccgagg agtccgcagg taaaatgaag
     4201 gagaacgcgg aaggaaggtc agggaagaat cgttctgatg agatgcccaa agacggaccg
     4261 ccggccgagc gttaccgtca accgggcgag cagggtccgg atagtatcaa aggggccaaa
     4321 tacgtgactg tgcaattacc ggaagaaatc gcctccggta ctagcggcgt aacggtctcg
     4381 agcaaggacg gcaaagaaag caaaaatcga ccaagagtac cgataagcaa cgttccacta
     4441 cccgctcaca ttcctgaggc tcctgcggag aaacagcgaa tgcccctcga gtaccgcgat
     4501 cttattcgct agatcagaaa ggcagcgcga tggacccaga aaagtttcag gagtcgttca
     4561 agcgtttgga ggcggaggtg cgaaaggtca tcgtcggtca tgatgacact attcgcaaag
     4621 tgttaatcgc attttttgcc ggcgggcacg ttctattgga aggggtgccg ggtttgggga
     4681 agacgttact agtcaaaacg ttgagccatg ccctgggagt ttcatttaag cgcattcaat
     4741 tcacgccgga tctgatgccg tctgacatcg tcgggacgca ggtgttggcg gaaatcaatg
     4801 gccgcagaga gttccagttc aaacagggac cgatcttcgc ccatgtggtg ctcgccgacg
     4861 agatcaaccg cgccacgccc aaaacgcaat cggcggtttt ggaagccatg gaagaaaagc
     4921 aagtgaccgt tttcggcgaa tcccacgcct tggaatcgcc gtttatggtg ctggcgacgc
     4981 aaaatccgat cgaattggag ggtacatatc cgctacccga ggctcagctt gaccgatttt
     5041 tttttaaact tctggtgatg ccaccgacgc cagtcgaact cagagaaata ctgaagcgaa
     5101 ctaccgggac cgaaaccgcg gatatgcaag aagtcttgcc cgaagacgag ggcaaactga
     5161 ttaacgagat gaagcagcta ctgcggcaag tcttgatcgc gccgcctatc gaagattatg
     5221 tcgtgcgact ggtgcacgcg acccagccgc agccgcaaaa cgccgcaaac tcaccggtca
     5281 ggcaatactt gcgctttggc tcaagcccgc gcggcgcgca atcggttatt ttgggagcga
     5341 aaggccacgc tctggccgat ggtcgagttc acgtcagcta cgacgacgtt gagaatgttc
     5401 tctacccggc cctgcgtcac cgtatgattt taaatttcca agccgaagcc gagaacgtca
     5461 cggcggatca aatcatggat gaggtgatga agcaggtggg gcggaactag catcccgagt
     5521 tgacctgtcc cagctcttgc gcaggaaatt gctttatcgc gattccagaa gcggcaaatt
     5581 attcgatctt gtttttaatg ggcgggatcg atttcagata gtcggcaata gccaacgcat
     5641 cttcgcgccg catatcttta tacccgtgcg gggttccctg gatgacttca tgcataaggc
     5701 cttgaacatt gtcgaagtct ggctttgttc ctgtgattaa tagctcggca atatcttcgc
     5761 gtttccaatc gccgatccct gtttctttgt caggcgtaat gttcggcacc ttctctccga
     5821 ggggaccgtc cttagcgccg gcgccggcaa gatacagtga actcttttga acgccgatcg
     5881 agttcctggg tgtgtggcag tcgccgcaaa tcgagacatg acgaaccaga taacgcccgc
     5941 gttcaacacc gctcttaggc gcttgcgccg gcgagttcga ggagccgccg aagagattga
     6001 gaaaaacagg cactcccaca ctgcgcacca agggaaccca cgttttcaga ttaggagtcg
     6061 cttttttcac cggtttcaaa gtcctcatgt aggcgatcag ggctttgaga tcttccggcg
     6121 ccattcccga ataggcttcg tagggcatca caggtaccag cctgctgcca tcgcgccttc
     6181 tcccggcgtt catagcgtcg tagatttgcc gctcgctcca atctcccagc ccggtctcct
     6241 tgtcctgggt gatgtttgtg ctgaacaccg tgccgaatgg aataggaaag gcccggccgc
     6301 cggtgtggta ggtttctttg ggtacagtat gacaggcgca gccgcccgcg atggcgaaga
     6361 ggtactggcc tttcgcgatc agctctttat cggcttgggc gtacagtgta gctgatgtca
     6421 gaaaaaaacc gacggttagg aataatagaa ctcgcctcgt cctccgcatt cgtaaaactc
     6481 gttgcatgtt ttaacgtgag gcctcctcgc gaagtttttt atccaaaaaa ctcaacgtag
     6541 catgaaacac tctgttgatc ggcgcctgct ctccagtctc ctcaagcaaa cgagtgacaa
     6601 tgccgttaag cgcgtcgcat tcgagc
//
LOCUS       DASUVS010000360        27231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1463363, whole genome shotgun sequence.
ACCESSION   DASUVS010000360 DASUVS010000000
VERSION     DASUVS010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 27231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27231
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463363"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VF182_19175"
     CDS             <1..552
                     /locus_tag="VF182_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439250.1"
                     /translation="AAGSVVANLVLPGVGTILAIGLGAGAFGIGGAVAGAATGGALEN
                     QLSPGLPKDELFFYEDALRQGRTVLIARSQDDDVIEKGRAVIERNGAESVDAARDKWW
                     IGMRDAEEAEYDAPDGDFHRNESRYRSGFETALHPDCRGKTYDAAAPKLRELRPDVCE
                     DETFRRGYERGRAYYIEREKATP"
     gene            892..1662
                     /locus_tag="VF182_19180"
     CDS             892..1662
                     /locus_tag="VF182_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439251.1"
                     /translation="MKVHYLIFTALLLTPAPLIAADGTARKGDTDRSRYSDRDQRKTY
                     DDEEQKLEQALKAGQDKNFYRRELEKMGYQITSVNYDKPDYAEYEIVKGTNSYEAQID
                     FDKSGKATKIDIAPNLWRTDSTKAALRGKKVSAPKGAIANPERYSDRDRRKAYDSEEE
                     KLEKTLKTGETKQTYRSQLEKMGYKVTSVNADKPDYVEYEIVKGNDTYEVQIDFDKNS
                     GKAIKVDVTSNLWQADATDKALSQRQEKAANRQNSKQK"
     gene            1797..1949
                     /locus_tag="VF182_19185"
     CDS             1797..1949
                     /locus_tag="VF182_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEX6439252.1"
                     /translation="MSTLLIIILILLLIGALPTWPYSGGWGYFPSGGLGLILLIIIIL
                     VLMGRL"
     gene            1998..3575
                     /locus_tag="VF182_19190"
     CDS             1998..3575
                     /locus_tag="VF182_19190"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEX6439253.1"
                     /translation="MRKVLLWSAAILGLLIVLVIIAAFFLDEPLRRRTEAKINASLKG
                     YTVRIEKLDFHPIGFSLDLENSVITQDANPEPAVAEIPNLTASVNWRALLSGHVVADF
                     EINNPKLFLNLKQSQNEIKDETPVRERGWQDAVQAIYPLKINELVITGGELTYVDQGQ
                     YRPLELTNVNLTAYNIRNVRSQEGEYPSEVYIEGTVFKNGKIVLDGNADFLAEPHIAI
                     KAAIKAEQIELDYFRPITERYNFSVRRGVLSTNGSIEYAKNKKIISVPTLNVHNLVAD
                     YVNTKPATSPTKELSKKADKVIREHSNEPTLELDVNDVNISDSELGIVNKASEPQYRL
                     FVTGAQVKIKNLSNQGEDGTATITASGTFMNSGKTRLVARLRPKGKSPNFDLQLAIDQ
                     SELKALNPLLRAYIGADVVAGQFSFYSEIAVRDGSINGYVKPLFKDIDVYDPKQDRKK
                     GIFTKLYEGILGGLSWILENRPRQEVATTTKISGKLSDPQTSVTDMILGLVQNAFFRA
                     ILPGLEREIEVGGRKGR"
     gene            complement(3595..4395)
                     /locus_tag="VF182_19195"
     CDS             complement(3595..4395)
                     /locus_tag="VF182_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX6439254.1"
                     /translation="MQVAPEQKSNHAFYRDVLEILRHAEVPFLISGGFALQQFTGISR
                     EVRDLDIFVLEKHIRAALEALAQGGYPTELTFSHWLGKVYNHNRTEYVDLIFSSGNGL
                     CKVTDSWFQRAIPSEVFEIQVFLCAPEEMIWQKAFIMERERYDGADVAHLIRTQSDAI
                     DWERLIGLFGEHWRVLLSHVILFEFIYPAERAKIPRWVKQRLIVLLQRECDEPSTEEQ
                     VCQGPLLSRSQFRVDTEDWGYKDARLNIMSAEEVAHWTEAAAEKEEKP"
     gene            complement(4410..5195)
                     /locus_tag="VF182_19200"
     CDS             complement(4410..5195)
                     /locus_tag="VF182_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6439255.1"
                     /translation="MAYALLALASMTDNVVHIAAVADLHCKSNFAGKFRTLFSQISDQ
                     ANILLLCGDLVDYGLPEEAHVLIKELSELTKRIPVIAVMGNHEFESGKIDEVQKILTD
                     GGVLLLDGEACEMEGIGFAGVKGFAGGFGERLLAPWGEAIMKKFVHEAVEEALKLESA
                     LAKLRTTHRIALLHYSPIAATVMGEPLEIYPFLGSSRLEEPLNRFSVTAAFHGHAHHG
                     APEGKTKTGVPVYNVAMKLLEYSFPGQLPYRILNLPRGNGAAK"
     gene            complement(5378..5575)
                     /locus_tag="VF182_19205"
     CDS             complement(5378..5575)
                     /locus_tag="VF182_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX6439256.1"
                     /translation="MNWDQMKGNWKQFKGKAKEQWGKLTDDELDQIEGKRDQLVGKIQ
                     KQYGLSKEEAERQVGDWESRL"
     gene            6368..6601
                     /locus_tag="VF182_19210"
     CDS             6368..6601
                     /locus_tag="VF182_19210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439257.1"
                     /translation="MKTAAERVRQTGPRVEAAVHSTAETIAEKLDRGGAYFRERQYEE
                     LLNKATRYIRQNPVTSLIVGVVSGLLLARKIKR"
     gene            6671..7111
                     /locus_tag="VF182_19215"
     CDS             6671..7111
                     /locus_tag="VF182_19215"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX6439258.1"
                     /translation="MEPSQNEIRREIEATRAAMAEKIETLEARVGDVIKEAKRSVDPK
                     YQTEKRPWLMVGLAVATGYLFEGIIFGKAPPRGRGRKFSVDPERRAYLEGKQKAAGGG
                     ILRSIATAAGVALARDYATKIVSGRGLSSRPNEGRPKVRSPYEL"
     gene            7328..7615
                     /locus_tag="VF182_19220"
     CDS             7328..7615
                     /locus_tag="VF182_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439259.1"
                     /translation="MKSQRVRWEGQSFWQLVFGQSKVNGARYDGENLTTSPWRWPTNT
                     YLQRRRQLLKNSQAFDQADRDEADGGFYDTRYDTGNRALVEFREPGSLRGN"
     gene            7833..9392
                     /locus_tag="VF182_19225"
     CDS             7833..9392
                     /locus_tag="VF182_19225"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX6439260.1"
                     /translation="MSVKKILEPEKNCWTFSEVTQSGLLIDGRDYYRAFYQAARGAED
                     YILISGWQFDSDVALLRGSDADDAADSVQFLTFLNNLCEQNQRLHISILAWDFTVLYS
                     LDREWFQDWYFNWTTNERFTFCFDCCEFFGSAHHQKFVVIDGKLAFVGGLDICSGRWD
                     DRDHPVDSPARVNSDQNGYGPFHDVQSYHVGPAAQKLAELFAARWKCVTTAELTLPTP
                     SSNYQLPFRPTVAIPAESIAISRTQAGTAGQQKPIQEIRRLFLDAIDAAENLIYVENQ
                     YFSSNALYKALVDRMLDARRSHLEIVLIIAKEAEAFVEQLSIGVVQEKLLRELQSVAA
                     ETGHSFGIYYPASVRGDGKESATYIHSKLFLVDDRFLSVGSANMNNRSLGLDTELNVA
                     WEAPSTDSEVGRAIRAARVDLLAEHVGVRDPQVIENLIRSRGLVKYLNTLADSRSYRL
                     RHHPLDIVSEDYLWVTSVLPEGLPFDPEGPFYQEDRYEGISAAEDSFFSRGVSNLKNW
                     LLSLGQTLSPR"
     gene            complement(9468..10994)
                     /locus_tag="VF182_19230"
     CDS             complement(9468..10994)
                     /locus_tag="VF182_19230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEX6439261.1"
                     /translation="MNAPWEWNDINEQKAKRRFGIRHYRAGQRKLIQAVLEGRNTLGI
                     LPTGGGKSLCYQLPALFLPRAVLVISPLISLMQDQQEKLLERSIPAAELNSTLTASEE
                     EENIKAIQSRQAELIYVTPERLENAGYLKILKKGGVSLVVVDEAHCVSQWGHDFRPAY
                     LSLRNTIRELGGPPVLALTATATDDVAADILKQLDIQDAEVIGIGIERKNLAFEVIRT
                     VNEEVKQDRLRELLREESGGAGIIYVPTVKQVDALCKWLSDEGFRAGQYHGRMKMTLR
                     ETNQQAFMANEIDVMVATKAFGLGIDKQDIRFVIHYAVPDSIETYVQETGRAGRDGKP
                     ARAILFYRLEDRRVQSYFLGGKYPRRDESLALYRTLEQMPRPEDLADSSAEQWLAEIT
                     DLTEKRVKVILALLESTGIVQRKPHVSKIRDFNTDGEFAAFLEEYEQRHNGDRQRLDA
                     MMLFGQITTCRMRYLLEYFDEEVDSDCSRCDNCRAGQEIANNSFRRPILSGGAVVAAN
                     "
     gene            complement(11064..11819)
                     /locus_tag="VF182_19235"
     CDS             complement(11064..11819)
                     /locus_tag="VF182_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX6439262.1"
                     /translation="MLRRVESLRKFSIGAKDGEIGNVVEFFFDDDNWTIRYLVAHTGT
                     WLSGRKVLIPPISFGAVDWDGNIFNVNLTRQQIANSPSIDTDKPVSRQHESSFYDYYN
                     FPYYWATAALANPAAIDPAGIAASGVETAVPGRPAGSEDIHLRSTEDIIGSYIETTDG
                     DLGHLDDFIIDDQNWVIRYLLIDTTNWLPGKKVLISPEWVKDMVEVDSRVYVDLTQEQ
                     IKTSPEYDDTTALDRDYESRLYRHYGRSRYWSD"
     gene            12169..12546
                     /locus_tag="VF182_19240"
     CDS             12169..12546
                     /locus_tag="VF182_19240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6439263.1"
                     /translation="MAGKILIVEDDTITRRNLSVLLSDEGYDVDQAGDGVQALEILAA
                     QPFDLVLSDIVMPRMDGLKLLQQLQFVAPQMPVMIMTSYVSDSLSTVPAGAAEFIRKP
                     FVLDDFLFKVQRALDKTVQAPSL"
     gene            complement(12861..13556)
                     /locus_tag="VF182_19245"
     CDS             complement(12861..13556)
                     /locus_tag="VF182_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX6439264.1"
                     /translation="MFGKSRSSHPVTIPFSDRRQAGKKLATQLATYSNRSDVLVLALP
                     RGGVPVAFEVARALRAPLDVFLVRKLGVPGHEELAMGAIAPGGVRVLNEEVTSQMQIS
                     SSAIDEVAKREEQELQRRERLYHGARAAPDARGRTVILIDDGLATGSTMRAAARALRG
                     MQPSKIVVAVPVAAAETCADLHNEVDEVICLATPEPFMAVGAWYSDFEQTSDQEVREL
                     LDRIASHEQDDST"
     gene            complement(13811..15340)
                     /locus_tag="VF182_19250"
     CDS             complement(13811..15340)
                     /locus_tag="VF182_19250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX6439265.1"
                     /translation="MDSNGDGSGDLRGIISRLDYCQWLGVDAVWLSPIYPSPMVDFGY
                     DISDFTEIHPLFGSLGDFDALISEAHRLGLKVLLDYVPNHSSDQHFWFLESRSARNNP
                     FRDWYIWRDPAPGGGPPNNWLSAFGGSAWEWDENSGQYYLHSYLKEQPDLNWRNPALQ
                     KSMLDVLRFWLDRGVNGFRVDSIAHLMKDPEFRDNPLNLTWDPAAGPYFRYLPVHSRD
                     HPDTAAVLRRMRRVVDQYEDCVIIGEAYLSLERIVAYYGAGVHLPFNFALITSPWTAR
                     EVHRAIQIYESLLGPDHWPNWVLGNHDRHRIATRVGRAQARVAAMLLLTLRGTPTLYY
                     GDEIGMQDVPIPAELVKDPWELNVPSLGLGRDPERSPMQWDSSYHAGFSTATPWLPVA
                     SDFQRFNVAVESQEADSVLSLYRRLIALRAEQPALHSGAYGKVLCDDHVLAYSRRSRS
                     ENFLIVLNFTGEPRTFEHAGLRGRINLTTGIDRNSEPVAERLQLRPNEGVILSLELHD
                     V"
     gene            complement(15459..16628)
                     /locus_tag="VF182_19255"
     CDS             complement(15459..16628)
                     /locus_tag="VF182_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEX6439266.1"
                     /translation="MKADLALTRKPAGKRLGAIVSPDPLESAKAASLRYVTDDKPGIS
                     RQRRGAAFQYIDPSGKVIRDPGELQRIKSLAIPPAWREVWICPLPNGHLQATGRDAKG
                     RKQHRYHPRWREVRDENKYGRILAFAKALPLIRQRVDNDLRLPGLPRDKILATVVRLL
                     ETSHIRVGNEEYARQNKSFGLATLRNRHVNVSGSTIRFEFRGKSGVQHALDISDKRLA
                     KIIKRCQDLPGYELFQYVDDDGERRTIDSSDVNEYIRQVAGEEFSTKDFRTWAGTVLA
                     AQALQKFETAASDSESKRNIVQAIEGVAKKLGNTKAVCRKCYIHPAVIQSYTDGSLLR
                     ALRRRLKKEITDSHGLEPEEAAVLKLLKAGITSDSKAPVKVAANGAKGLRAIAHT"
     gene            complement(16787..17089)
                     /locus_tag="VF182_19260"
     CDS             complement(16787..17089)
                     /locus_tag="VF182_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439267.1"
                     /translation="MDNYEGAYGGQSQEPSRTEDQFTKSVEKYTGAIPSSAFLGVAIG
                     AMALSLAMQIGGRGKWGNFIGQWVPTWLIIGLYNKLVKLEGHDQSDRGDGYSRYSS"
     gene            complement(17397..18854)
                     /locus_tag="VF182_19265"
     CDS             complement(17397..18854)
                     /locus_tag="VF182_19265"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6439268.1"
                     /translation="MRGREGNMRYIALACDYDGTLATDGQVSSGTLAALQRLLASGRK
                     LILVTGRQLDDLFSVFPRWDIFEWIVAENGVLLYHPATDEKKLLASPPPAKFMARLRE
                     RGVPISVGEVILSTYHPHESLVLDTIRELGLELQLIFNKGAVMILPSGTSKATGLTAA
                     LKQLGLSPHNVVAIGDAENDHALLALCECSVAVANALPMLQEKADFVTTGANGQGVTE
                     LINEIIANDLRDRDTALTRHHLLLGTSVDGRQVRLSPFASNILLAGSSESERRNVAAH
                     FIESLCRQGYQFCLFDPRGAFEGVPGGIALGRHKGAPTVDEIIRLLKDPAENAIINLS
                     GVAVRQRTSFIQELFARIQQLRARTGHPHWIIVDEAQDVLSRSWQPAPLDIPQQFEGM
                     MCVTGNPAAVARSLWSAVNIVLALDPSGATAFSQLAGLSRDAMLEIPAIPANAGEAIV
                     WLRDRGAPFRIKLASSRDRRHSSPSATATMPQRVD"
     gene            18985..21135
                     /locus_tag="VF182_19270"
     CDS             18985..21135
                     /locus_tag="VF182_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX6439269.1"
                     /translation="MNEVIRLPDEFYILATASAAEESRVLKMGESFGIFDRYGDILQY
                     GLGEQGIYHEGTRFLSYLELRLGNIRPFLLSSMIKEDNILFTVDLTNPDVYVDGTIAL
                     RRGDVHLYRSKFIWQAICYERLEVVNYSLSPIEISFAIRFDADFADIFEVRGVKREKR
                     GTRLEDQIDAKRVVLSYLGLDDIVRQTIIEFSPAPDKLGPSEASFHRVLNPKKHEAYL
                     VSYACKSNRSAPVRRPVELALREAERALTAYKAEECQIRTSNHEFNGWLDRSYADIHM
                     MITDSQGFYPYAGVPWFSTVFGRDGILTSWELLWLFPRVARGVLSYLAENQATEVISE
                     RDAEPGKILHETRRGEMANLGEIPFDRYYGSTDSTPLFIMLAGAYVARTNDAEYARWI
                     WPHVERALHWIEKYGDTDGDGFVETFRRSSHGLVQQGWKDSWDSVFHRDGTLAAAPLA
                     LCEVQGYVYAAKHAAAEIATLLGHSEEALRLASAAERLKEQFNQMFWCDELSTYALAL
                     DRDKRPCRVRASNAGHCLFAGIATDEYARRTAKTLMSEDSFSGWGIRTLSAGEVRYNP
                     MSYHNGSVWPHDNAVIAAGFARYGLKEPISRVITGLFEASLYLDMHRLPELFCGFPRR
                     EGEGPTLYPVACAPQAWAAGSVFLMLQACLGLLVHASEGKVHFDSPELPECIDTIHLK
                     NLQIDSSLLDLVVDRSFQGIGVERRQGNASIVIS"
     gene            complement(21049..22155)
                     /locus_tag="VF182_19275"
     CDS             complement(21049..22155)
                     /locus_tag="VF182_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6439270.1"
                     /translation="MRIAQVSPLYESVPPQTYGGTERIVSYLTEELVAQGHEVTLFAS
                     GDSVTAAQLIAARKRALRLDEVFDPLAHHYVMLEEVFERAARFDIIHFHTDYLHFPLA
                     RRHSAAHVTTLHGRLDLPDLMPVYKKFSEMPLVSISNAQRKPLPRANWQDTIYHGLPL
                     GLYECSEKPGDYLAFIGRISPEKRLDRAIEIAARSGMKLRIAAKTDAADREYMEQVIR
                     PILSASSAELIGEIGDKDKKEFLANAYALLFPIDWPEPFGLVIIEAMACGTPIIAYRC
                     GSVPELIQEGITGYIVDDIEGALEALGKIPSFDRRRCRQVFEERFSATRMAHDYLSVY
                     RRVIKASADHNARIALAALNSDTLETTIDYQIEK"
     gene            22701..23084
                     /locus_tag="VF182_19280"
     CDS             22701..23084
                     /locus_tag="VF182_19280"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEX6439271.1"
                     /translation="MKAPQFWIALLRIVVGAWFVKAVWTKLSLAIAWRIIPYPAVSQR
                     FIGFHPKRVADFAAGDPVEPYKQFIQETVLPNAQLFARLQAYGEVVVGFGLLTGLCVG
                     ITALICLLLCINYGLATHMGWLLSG"
     gene            23226..24167
                     /locus_tag="VF182_19285"
     CDS             23226..24167
                     /locus_tag="VF182_19285"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439272.1"
                     /translation="MSLAVLALLPAGTKAGAAELRVFVTNEKSNNVTVIEALSGKVLA
                     TIAVGQGPRGVANSTEGGRVFVANSNSNNVSVIDTASLKVIDSFPAGTDPEGITVDGR
                     NRLYVANENESALTIIDISKRQVVKRHEVGLEPETAVLSPDERWVTVSNESSSDFYFI
                     NAEPAAMAGKVGVPRNPRGMRLSADSRRLYVASEQEPMVSIIDVAQRKLIGSMPTGGE
                     RPVDIVICPNGQRIYVSHGRSEDVRVFDAATWKLLATIPVGPRAWWMTQTPDGKFLYV
                     TVGRANEVVMIDTRSNTIARRIPVGQLPWGIAIAEVK"
     gene            24833..25015
                     /locus_tag="VF182_19290"
     CDS             24833..25015
                     /locus_tag="VF182_19290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439273.1"
                     /translation="MKKKLSAELERLEIEHGHEWRGIIQEQFGRLWLRLNMPEKKPAK
                     EPLTLPKIRGEGGKVA"
     gene            25832..26305
                     /locus_tag="VF182_19295"
     CDS             25832..26305
                     /locus_tag="VF182_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018295110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439274.1"
                     /translation="MRKRITNHDAQKYAAVDEGWLDLEKVAQVEVSSEEAQHPIEAAL
                     LPGVSAGWRAAAPGQQLVRLMFDKPQNLRLIHLVFEEHGQERLQEYTLRWSSGVGQAY
                     REIVRQQYNFSPPTTTTEVENHTVNLDSCAELELSIIPDLRGSAAHAAVVEFRVA"
     gene            26351..26518
                     /locus_tag="VF182_19300"
     CDS             26351..26518
                     /locus_tag="VF182_19300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439275.1"
                     /translation="MIEANSKCPKCGAGIMEIVPEHRAIAPDLPEDNELKARCDKCGY
                     EDQVEVVATDE"
     gene            26900..27130
                     /locus_tag="VF182_19305"
     CDS             26900..27130
                     /locus_tag="VF182_19305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6439276.1"
                     /translation="MLCDIALPWMDRHAIARKIRQDPELKDVYLIAISGFGQDEDQQL
                     ARDAGFNEYRVKPVDLRSLDQLIKHRQGPSRR"
BASE COUNT         6320 a         7252 c         7389 g         6270 t
ORIGIN      
        1 gccgcgggaa gtgtcgtggc aaacttggtc ttgccagggg tcggaaccat actggcgatc
       61 ggattgggcg ccggggcatt tggaatcggc ggcgcagtgg ctggcgcggc caccggtggg
      121 gcacttgaga atcagctttc accgggatta cccaaagatg agctgttttt ttatgaagat
      181 gctttgaggc agggccgaac tgtgttgatt gccaggtcgc aggacgatga tgtgattgaa
      241 aaggggagag ctgtgatcga gcgcaacggt gcggaaagcg ttgatgctgc gcgtgacaag
      301 tggtggatcg gaatgcgtga tgccgaagaa gcggagtatg atgcgcccga cggcgacttt
      361 cacagaaacg aaagccgcta ccgcagcggc tttgaaacgg ctcttcatcc tgattgccgc
      421 ggtaaaactt atgacgctgc agcaccaaag ctgcgtgaat tgcgtcctga cgtttgtgag
      481 gacgaaacct ttcgtcgcgg ctatgaacgc ggtcgcgcct attatatcga gcgtgagaag
      541 gccacccctt agcacttagg cgaatcttgt aaagctggcc gcacggccgt gttagtaagc
      601 tgccgtcccg gccctcctcc atcttcggaa tcattgctgc gcacaaccgt atcggtgtcg
      661 ctgatgtgag aaagactcgt gttaactgtg ccgcaattgc tacaatcgct cttctccact
      721 gtgaatccgg ccgcatattc aataacaatc tgtaaccgct gaatagggcc agctctgagc
      781 ctccaaacaa ttagttcctg gcatatcgat tgctctactt acctttcgaa gtttcgaata
      841 taacaacgga tggctgttca tcataatgag tcaactagag gaggaatcaa catgaaagtt
      901 cattatctga tattcacagc tttgctgctc acgcctgccc ctctcatagc ggcagatggc
      961 accgccagga agggcgacac cgatcgaagt cgctacagtg accgtgatca gagaaaaact
     1021 tacgatgatg aagagcaaaa actggagcag gcgctgaagg ctggacagga caaaaacttc
     1081 taccggcgag agctggagaa gatgggctat caaattacct cagtgaatta tgacaaaccc
     1141 gattacgccg aatacgagat tgtgaaaggc accaattcat atgaggcgca gatagatttt
     1201 gataagagcg gcaaagctac aaagatagac attgctccga acctatggcg aacagattct
     1261 accaaggcgg ctttgagggg gaaaaaggtt tccgccccga agggcgcgat agccaaccct
     1321 gaacgttaca gcgaccggga taggcgcaag gcttacgaca gcgaagagga gaagctggag
     1381 aagactttga agacgggtga aaccaagcag acctatcgca gccagctcga gaagatgggt
     1441 tacaaagtta cttcagtcaa cgctgacaag cccgattacg tggagtacga gatcgtcaaa
     1501 ggcaacgaca cctacgaagt gcaaatcgac ttcgacaaga acagcggcaa ggccatcaaa
     1561 gtcgacgtga cttcgaattt gtggcaagcg gatgcaacgg ataaggcgct cagccagaga
     1621 caggaaaaag ctgcgaaccg acagaactcg aagcagaaat gataggcaga tcgatggaaa
     1681 ttcgattccg aaccggggag cgcgtttcca taagcgcgcc ccggccgacc gcaggatgtg
     1741 ccagcacagt tcgagatgaa tgatcgagtc tgactagcaa tcgtggagga caacctatga
     1801 gcactcttct gatcattatt ctaatactct tgcttatcgg agctcttccg acatggccct
     1861 atagcggcgg ttggggatat tttcctagcg gcgggcttgg attgatctta ttgatcatca
     1921 tcatccttgt gttgatgggt cggctgtgat cggttcctga atgccgctgg cgacaaggtc
     1981 agcggcattt tttgtttatg agaaaggttt tactgtggtc agcagccatt ttgggtttgc
     2041 tgatcgtcct ggttatcatt gccgccttct tcctagatga gccgttacgc cgccggaccg
     2101 aagctaagat aaatgcatcc ttgaagggct ataccgttcg catagagaag ctggattttc
     2161 atccgattgg atttagtctc gatctcgaaa actcggtaat tacgcaggac gccaatcctg
     2221 aaccggccgt cgctgagata ccgaatctta ccgccagcgt taactggcgg gctttgctct
     2281 ccggccacgt cgttgcggat ttcgaaataa ataatccgaa gttgtttctc aacctcaagc
     2341 aatcccagaa tgagataaag gacgaaacac cggttcgaga gcgaggctgg caagatgccg
     2401 tgcaggcgat ctatccgctc aagatcaacg aactcgtcat caccggcggt gagcttacct
     2461 acgtggatca aggccaatac cggccgctcg aactcactaa cgttaatctc acggcctaca
     2521 acattcgcaa cgtcagatct caagaaggcg aatatccgtc agaggtgtat atcgaaggta
     2581 ccgtgtttaa gaacgggaaa attgttcttg acggaaatgc ggattttctt gccgagcccc
     2641 atatcgccat taaagcggcg attaaggcgg agcaaatcga actggattac ttcagaccca
     2701 tcacggaacg ctacaatttc tcagtgcgcc gcggcgtact gagcaccaac ggtagcatcg
     2761 aatacgccaa gaacaagaaa atcatcagcg tgccaacgct caacgtgcat aatctggtgg
     2821 ccgattacgt gaatacgaag cccgcgactt ctccgactaa ggaactgtcg aaaaaggcag
     2881 acaaggttat tcgagagcac agcaacgagc ctaccctcga attggatgtc aacgacgtca
     2941 atattagcga tagtgagttg gggatcgtca acaaggcaag cgagccccag taccgtctct
     3001 ttgtcaccgg cgcccaggtg aaaattaaaa atcttagtaa tcaaggggag gatggcaccg
     3061 cgacgattac ggcaagcggg acattcatga attcgggaaa gacccgtttg gtggcgcgcc
     3121 taagacctaa agggaagagc cccaacttcg atctgcagtt agccatcgat cagtcggagc
     3181 tcaaagcact caatcctttg ctccgagcct acatcggcgc cgacgtggtc gcaggccagt
     3241 tctcgttcta ttcagagatc gcagttcgtg acggatcgat caacggctac gtcaaacctc
     3301 tgttcaaaga catcgacgtt tacgatccaa aacaagatcg taaaaaaggc atcttcacca
     3361 agctgtacga aggcatttta ggcgggctct catggattct cgagaatcgc ccgcgccagg
     3421 aggtcgctac gacgaccaaa atctccggca aactttccga tccgcaaacc agcgtgacgg
     3481 atatgatcct gggccttgtg cagaacgctt tttttagagc gatacttccc gggttggaac
     3541 gagagatcga agtgggcggc aggaaagggc gctaaaaccc tgatgtagat aatattaagg
     3601 cttttcttct ttctcggctg ccgcttccgt ccaatgtgcc acctcttcgg ccgacatgat
     3661 gttcaaacgg gcatctttat agccccagtc ttccgtgtcg acgcgaaact gtgaacggga
     3721 aagaagcggt ccctgacaca cctgttcttc agtagacggc tcatcacact ccctctgcag
     3781 gagaacgatg agtctctgct tcacccagcg aggaattttc gcgcgctccg ccggatatat
     3841 gaactcgaac aggatgacat ggctcagaag cacgcgccaa tgctcgccga acaagccgat
     3901 taatcgttcc cagtctatgg catcgctttg cgtccgaatc agatgggcga catcggcgcc
     3961 atcgtagcgt tctcgctcca tgatgaaggc tttctgccag atcatttctt cgggggcgca
     4021 gaggaagacc tgtatctcga acacctcgct agggatggcg cgttggaacc agctgtcggt
     4081 gactttgcac aagccatttc ccgagctgaa tatcaggtcg acgtactcgg tccgattgtg
     4141 gttgtaaact ttgccgagcc aatgggaaaa tgtcagctct gtcggatagc cgccttgggc
     4201 aagcgcctcg agcgcggcac ggatgtgctt ttcaagcaca aagatatcca gatctcgcac
     4261 ttctctagag atgccggtaa actgttggag cgcaaacccg ccgctgatta aaaatggtac
     4321 ttccgcgtgt cgcaaaatct ccaaaacatc ccggtaaaag gcatggttcg atttctgttc
     4381 aggcgcgact tgcacgacgg atcacccttt catttcgccg cgccgttacc gcggggtaaa
     4441 ttcaaaatcc gatagggcag ttggccggga aaactgtact cgagaagttt catagcaacg
     4501 ttgtaaactg gcactccggt tttggttttt ccttcaggag cgccatgatg cgcgtgaccg
     4561 tgaaaggcag ccgttacaga aaatcgattt aacggctctt ccaggcggct cgaccccaag
     4621 aacggataga tttccagcgg ttctcccatc actgtcgccg caatcgggga gtagtgcaac
     4681 aacgcgatac gatgggtcgt gcgcagcttg gccagcgccg attcgagctt tagagcctcc
     4741 tccacagcct cgtgcacaaa cttcttcata atggcttcgc cccaaggggc caatagtcgt
     4801 tcgccaaagc cgcccgcgaa tcctttgacg cctgcgaaac cgattccctc catctcgcat
     4861 gcttcgccgt caagcaggag aacgccgcca tccgttagta tcttttgcac ttcatcgatc
     4921 ttgccggatt caaattcgtg attacccatg acagcgatga ctggaatccg tttggtgagc
     4981 tcgcttaatt cttttatcaa tacgtgagct tcttcaggca acccataatc aaccaaatcg
     5041 ccgcacagca aaagtatgtt ggcctgatcg ctgatctgag aaaatagcgt tcggaacttg
     5101 cccgcgaaat tacttttgca atgcaggtcg gcgaccgcgg cgatatgaac cacattatcg
     5161 gtcatcgagg ccagtgccag caaagcatat gccacggcag ccgaagctct gagaagtgcg
     5221 ctttgtaaca aaggtttgga gtcgccggtc ctatagagag gagtccatct gaatggagat
     5281 cagcggaatc gaatcgtaaa cagtagtcgg gctgtcgaac gggctacagc gatgatctcg
     5341 cgatcaggaa aacgctgcgc ccgtccgcgc tcgctcgtta cagcctgctt tcccagtcac
     5401 caacttgacg ttcggcttcc tctttgctca ggccgtattg cttttgaatc ttaccaacta
     5461 gctggtcgcg cttaccttcg atttgatcga gttcatcgtc agtaagtttt ccccactgtt
     5521 ctttcgcctt ccctttaaat tgtttccaat tgcccttcat ttggtcccag ttcatcaggt
     5581 agccctccta tagatgtttt agaaataggt gtttagaaag agcagaaaaa gagcaatgga
     5641 tgccgacagc aatcgattga gccacccggc tgcgaacgtc aggtacaatt ttccatgtat
     5701 cggaaccgcc tcgagcaaag gttgagctgt cttgtaattg gcacatacca gtagcgcgga
     5761 ccaggagtgg ccatgcatgt ttctggcccg ggggtttgat cgcgctctct aaggaggacc
     5821 cgttggttga gttctagcct cctgatgttt ttcgattaca gcaatccttc tcatgctgtt
     5881 cggcttctgc ccgtgaatta aatgtctcgc cgcacgaacg acaagggaat tcctcgccat
     5941 cggtttccgt gcgctccggt aagggtgtgg catctttcat cgcgtcctcc cgatttcata
     6001 ttcagcaaca catatgccac cgccaaactg attctcttct tctggttgga atatcttttg
     6061 cggagctagg gcgtggttta ggacctgccg gtgccgcgct cgcaggtgta tggttgcgct
     6121 ggcttgccgc cagaactggt ttatgcagca tgcgaattgc gctggcaaat cctgggcaat
     6181 gtttgatatg aacagctgga caacgtttta agtgaaaggg gtgaagctga acatggaaca
     6241 caacggctca gacagagtat cagacaaagt aagagatctt gcgtcgcaaa tgggtgagcg
     6301 tgccgggtct gcaaaggagc aggcgtcgga ggccgctcgt cgcacggcgg ccagagtcgg
     6361 tgatcaaatg aagaccgcgg cggaacgagt gaggcaaaca gggcctcggg tcgaggctgc
     6421 cgtgcacagc accgccgaga cgattgccga gaagctggat cggggtggag cttactttag
     6481 agagcggcag tacgaagagc ttcttaataa agcgaccagg tatatacggc aaaatcccgt
     6541 gacatcgctg atcgtcggcg tcgtcagcgg tttattgcta gcgcgcaaaa taaagcgcta
     6601 attgtcgagt tcgcagtcac cagtttttct cagttagaag aacaacgagg taaagcgtaa
     6661 aggacgtcag atggaaccat cgcagaatga aattagacga gaaatcgaag ctacgcgcgc
     6721 ggctatggca gaaaaaatcg agacgcttga agcacgtgtc ggcgacgtta tcaaggaagc
     6781 gaaacgttca gttgatccca aataccagac ggaaaagcgc ccgtggttga tggtcggcct
     6841 ggcggtggca accggctact tattcgaagg aattattttc ggcaaagctc cgccgcgggg
     6901 gcgcggtcgc aagttttccg tcgatcccga acggcgagct tacctcgaag gaaaacagaa
     6961 agccgctggc ggcggaatac tgcgcagcat agcgacagcc gccggggtag cgctagcgcg
     7021 cgattatgca accaaaatag tttccggacg gggtctgtct tcacgtccaa acgagggtcg
     7081 accgaaagtt agatcaccct acgagctgtg atccggtaga acgtgccagt cgggagtgta
     7141 tcgaatctgg cacatagttt tggccaaatt ttcgagacag tcccatcagt agtacaggtc
     7201 gatccaccac gtgcggtttc tcgatacttt agactcattg aaaaggatca atgactggaa
     7261 tatcttttgc ctttcacggg tccagttgca gctattgtaa tctcgtccgg tgcacaggag
     7321 gtaggtcatg aagtcgcaac gcgtcagatg ggaaggacag agtttttggc agctagtatt
     7381 tggccaaagc aaggttaatg gagcccggta cgatggcgag aacctgacca catccccgtg
     7441 gcgttggcct actaacactt acctgcagcg gcggcggcag ctgttgaaaa acagccaggc
     7501 cttcgatcaa gccgaccggg atgaagccga cggcggtttt tatgataccc ggtatgacac
     7561 tggcaatcgg gctcttgttg aatttcgcga gccgggttca ttgcggggta attgaaactt
     7621 gatcgagctt tcttttctgc cattcgctgc aactttcgcc cgcttaggca tcctgagact
     7681 gatccaaaac attcggcgat tttttgttta gcgtcacaac cagcagcact gagagcgccg
     7741 cagcgctctg acgtagcgca tcttttaaga ttgtttttag ggggaatgat cccggcgggc
     7801 atcaattgat gcggagcagg gatgtacgtt ctatgtcggt caaaaaaata ctcgagccgg
     7861 agaagaactg ctggacgttc agcgaagtaa ctcagtcggg cctgctcatc gatggcaggg
     7921 attactaccg cgcattttat caggctgccc gaggagccga ggattatatc ctcatctccg
     7981 gttggcagtt cgatagcgat gtcgcgctgc ttcgcggcag cgatgccgac gacgctgcgg
     8041 attccgtaca gtttctcaca ttcttgaata atttgtgcga gcaaaaccaa cggttgcaca
     8101 tttccattct cgcttgggat tttacagtgc tctatagtct cgaccgtgag tggttccagg
     8161 attggtactt taactggacg acgaacgaga gattcacgtt ttgcttcgac tgctgcgaat
     8221 ttttcggttc cgcgcaccat cagaaatttg tcgtgattga cggcaagcta gcctttgtcg
     8281 gcgggctgga tatttgttcc ggccgctggg atgatcgcga ccatccggtc gacagtccgg
     8341 ctcgtgttaa ttccgaccaa aacggctatg gcccgtttca tgacgttcag tcttaccacg
     8401 tcggcccggc ggcgcagaaa ttggcagaac tgtttgccgc ccgctggaag tgcgtcacca
     8461 cggccgaact cacgctgcca acgccgtctt cgaattacca gctacctttt cgacccactg
     8521 ttgcaattcc ggcagaatca atcgcgatca gccgaaccca agccggaact gcggggcaac
     8581 aaaagccgat tcaggaaatc cgtcgtttat ttcttgacgc aatcgatgcc gcagagaatt
     8641 taatctatgt cgagaatcag tatttcagct cgaatgcgct gtacaaagct ctggtcgatc
     8701 gaatgttgga cgcgcgacgc tctcatttgg aaattgttct gatcatcgcc aaagaagccg
     8761 aagcttttgt tgagcagctt tcgattggcg tagtacaaga aaaactgctt cgggaactgc
     8821 agagtgtcgc cgccgagaca ggacactcct tcggcattta ttatcccgct tcggttcgtg
     8881 gcgacggcaa ggaaagcgcc acgtatattc attcgaagct tttcctggta gatgatcgtt
     8941 tcctgagtgt cggttcggct aacatgaaca atagaagcct gggacttgat acggaactga
     9001 acgtcgcgtg ggaggcgccc tcgactgata gtgaggttgg tcgcgcgatt cgagctgcgc
     9061 gagttgacct tttggccgaa cacgtcggag tacgagatcc tcaagtcatc gaaaatctaa
     9121 tccgaagccg cggtctggta aaatatctga acacgctcgc tgacagccgc tcttaccggc
     9181 tgcgccacca cccgctcgat attgtgtctg aagattattt gtgggtcacc tccgtgctcc
     9241 cggagggatt gccattcgat cccgaaggcc cgttctatca ggaagaccgg tatgaaggga
     9301 tttccgccgc tgaggattca ttttttagcc gcggcgtaag caatttgaaa aactggttac
     9361 tcagtctggg gcagacatta tcgccacgat gattcgtgga aattatttag gttctaaacg
     9421 cgcgctgagc gagcgatcgt cccatgccaa gtgctgttta acagttctca attcgccgca
     9481 acgaccgcgc cgccggacaa aatagggcgc ctaaagctgt tattcgcgat ttcttgtcca
     9541 gcgcggcagt tatcacagcg gctgcaatcg gagtccactt cctcgtcaaa gtactccagc
     9601 agataacgca tccggcaagt ggtaatctgg ccaaacaaca tcatcgcgtc tagacgttgc
     9661 cgatcgccgt tgtgacgctg ttcgtattct tcgagaaacg cggcgaactc tccgtccgtg
     9721 ttgaaatcgc ggattttgct aacgtgaggt ttgcgctgaa ctattcccgt actttccaac
     9781 agtgcgagaa taaccttgac gcgtttctcc gtcagatcgg taatttccgc cagccactgc
     9841 tccgcgctgc tatctgccaa atcttccggt cgaggcatct gctccagtgt ccgatacagg
     9901 gccaacgact catctcgtcg cggatatttg ccgccgagaa aataggattg aacgcgccga
     9961 tcctcaagcc gataaaacag gattgcccgc gccggcttac catcgcgtcc ggcccttccg
    10021 gtctcctgca cataagtttc gatcgaatcc gggaccgcat aatgaattac aaaacgaata
    10081 tcctgcttat cgatacccaa gccaaatgcc ttggttgcga ccatcacatc gatctcgttg
    10141 gccataaagg cttgttggtt ggtttcgcgc agcgtcatct tcatccggcc gtgatactga
    10201 ccggcgcgaa atccttcgtc actcaaccat ttgcatagag catcgacctg tttcacggtg
    10261 ggcacataga taatcccggc gccgcccgac tcttcccgta gcagttctct cagccgatct
    10321 tgcttgacct cctcgttgac cgtgcgaatc acctcgaacg caagattttt tcgttcaatg
    10381 ccgatgccga ttacctcggc gtcctgaata tcgagctgct ttaaaatatc ggccgccaca
    10441 tcatcagtgg ccgtggctgt cagagccagc accggcggac ccccgagttc tctgatagta
    10501 ttacgcagcg aaagataagc cgggcgaaaa tcatgtcccc actgcgaaac gcaatgagct
    10561 tcatcgacaa ccaccagcga cacaccgcct ttttttagga tcttcaaata gcctgcgttc
    10621 tccaatcgct ccggggtgac gtaaatcagc tctgcttgtc tactttgaat cgccttgatg
    10681 ttttcttcct cttcgctggc agtcagtgtc gaattcaatt ccgccgcagg aatgcttcgt
    10741 tccagaagct tttcctgctg atcctgcatc agtgaaatta gaggagagat gaccaaaacc
    10801 gcacgtggaa gaaataacgc aggcagctga tagcagagcg atttgccgcc tcccgtaggt
    10861 aggatcccca acgtgttcct tccctcgagc accgcctgaa tgagcttgcg ctgcccggcg
    10921 cggtaatgcc tgataccgaa acgcctcttg gccttttgct cgttgatatc gttccactcc
    10981 catggtgcgt tcatacgtgc ttctcccgct tttaatttac ttggttaatg atgggtgttg
    11041 cgatcggctg ctgctggtat gttttaatcg ctccagtacc gcgagcggcc gtagtgtcga
    11101 tagagacgcg attcgtagtc acgatcgaga gcggttgtat catcgtattc gggactggtt
    11161 tttatctgtt cctgggtgag atcgacgtag actctggaat cgacctcgac catgtctttg
    11221 acccattccg gcgagatcag cactttctta cccggcaacc agttggtcgt gtcgatgagt
    11281 aaataacgaa tcacccagtt ctgatcatcg atgatgaaat catccaagtg gccgagatcg
    11341 ccatcggtgg tttctatgta agagccgatt atatcttcgg tgctgcgcag atggatgtcc
    11401 tcggagccag ctgggcggcc tggcacggcg gtttccaccc cgcttgcagc tatccctgca
    11461 ggatcgatcg ccgccggatt cgcgagcgct gcagtcgccc agtaataggg aaagttgtag
    11521 tagtcgtaaa aactgctctc atgctggcgc gacaccggct tatcggtgtc gatgctcggg
    11581 ctatttgcaa tttgttgtcg ggtaagattg acgttgaaaa tgttaccatc ccaatccact
    11641 gcgccgaagg agatcggcgg tatcagaact ttcctgccac tcagccaggt tccggtatgg
    11701 gcaaccagat atcggatcgt ccagttgtcg tcgtcaaaga aaaactccac cacgttgccg
    11761 atttcgccat ccttagcgcc gatcgaaaat tttctcaggc tctctacccg tctcagcatg
    11821 gcttccacat acgtatagtc gcagaggcgg aactgtgatt gtgcaacgaa gatgccagtc
    11881 gggcgctgcc tttttacctg ggccggagtg tgttccgttg ttcggagatg gccctcagac
    11941 ggggcacgca ggaattggcg gtgtatcgaa aatgcgacga atcaaagctg aatctcgaat
    12001 tggatgttta gctgttcagt aaacatgccc aagggacggt gcctcgaccg aaggataacg
    12061 gcaccttttt caatttacgg caggaaatct ccaccaattg atggaggatc gtcgctcggt
    12121 cgccaacaga aacgattctg gactcatttt tgcggggcct acaaagctat ggcgggcaaa
    12181 atcctcattg tcgaagacga tacgatcaca cgaagaaacc tatccgtctt gctcagtgat
    12241 gagggctatg acgtagatca ggcaggtgat ggagttcagg cgcttgaaat ccttgccgca
    12301 caacctttcg atctagtgtt gtcggatatc gtgatgccgc ggatggacgg cctcaaactg
    12361 ctgcagcagc ttcagttcgt ggcgccacaa atgcccgtca tgatcatgac gagctatgta
    12421 tcggattcac tttcaactgt ccccgccggc gcggccgagt tcattcgaaa gccgttcgta
    12481 ttggacgact ttctgttcaa ggtgcagcgg gcacttgata agacagtcca agccccgagc
    12541 ctgtgaatct aatgtgtagg aactgtacgc acaggcagtt tcaatctcgc gcgccgttta
    12601 ccccccatgg tatgtgtcac aagccgacga tggcgcggga tcggctgaat tgtaagcggc
    12661 agccgtggtg tgttgcgcgc tgaaaatctc tcactgagaa gtccccacaa caaaatagat
    12721 ccgcgtggag atggattcga aatttcatcc ggccgtctta gcaccgatca cgcctccgga
    12781 aggcatagcg aggcgaccgg catcggtcag cgcgccgaaa cagcttgcct cggccgtgta
    12841 acgcttcctt ttacttcatg tcacgtagag tcgtcctgct catgagaggc aattcggtcc
    12901 aacaactccc gaacctcctg atcgctggtc tgctcaaagt ccgaatacca ggcgccaaca
    12961 gccataaacg gctccggcgt tgcaaggcaa atcacctcat cgacttcgtt gtgtaaatcg
    13021 gcgcaagtct cggcagcggc cacaggcacg gcaacgacga tcttcgatgg ctgcatccct
    13081 cgcagcgccc gcgccgccgc gcgcatcgtc gatccggttg ccaggccgtc atcgatgaga
    13141 atcaccgtcc gacctcgagc atcgggagcc gcccgagctc catggtacag ccgttcgcgc
    13201 cgttgtaact cctgctcttc ccgcttggcc acctcatcga ttgccgaact cgaaatttgc
    13261 atctggctgg tgacttcctc gttgagaacg cgaactccgc cgggggcgat tgcccccatg
    13321 gctagttctt cgtggcccgg gacgcccagc ttgcgcacca aaaaaacatc caggggagca
    13381 cgcagggcgc gggcaacttc aaatgccacg ggtaccccgc cgcgcggcag ggccagcacc
    13441 aggacatccg aacgattcga ataagtggct agttgggttg cgagcttctt gcctgcctgg
    13501 cgccgatccg aaaaaggtat ggttacggga tggctcgatc gagatttgcc aaacacgaga
    13561 atacctccgt acgaatcgtt gtgcggcggc gtttcagtaa gtgtatcccg gcgcaaagcc
    13621 catgccataa gaaggatgat cgcgcgtttg gctcaccgta ttcgattgat ttaactaaac
    13681 ttgggacagt ggaaatccgt gtccgggcga tgcacatcgc gaacgctttg aagtcggctt
    13741 ccgacgaccg aaaaaactca cgtcttaaac gatgcgcgcc ggataaggtc gcctcggcct
    13801 caaggggtgc ctaaacatcg tgcagttcta gacttagaat cactccctcg ttaggacgca
    13861 gttgaagtct ttcggcaacg ggctcgctat tgcgatcgat cccggtggtt aaatttatcc
    13921 tgccacgaag accggcgtgc tcgaatgttc tcggctcgcc ggtgaaattt agcacgatca
    13981 aaaaattttc cgaccgggag cggcggctgt aagccagcac atgatcgtcg cacaaaacct
    14041 tcccataggc accgctgtgc agtgctggct gctcggcgcg tagcgcgatc aagcgccggt
    14101 acagactgag aactgagtcg gcttcctgac tttcgacggc aacattaaac cgttggaaat
    14161 cactggctac cggcagccaa ggagtggctg tggagaagcc cgcgtgatag ctcgaatccc
    14221 attgcatcgg cgagcgttcc ggatctcgtc ctaagccgag ggaaggaacg tttaattccc
    14281 agggatcttt gaccagctca gccggtattg gaacatcctg catgccaatc tcatcgccgt
    14341 aataaagggt cggtgtgcca cgcaacgtga gcagaagcat cgccgcaacg cgcgcttgag
    14401 ccctgccgac gcgggtggca attcgatgac ggtcgtgatt tcccagcacc cagttgggcc
    14461 aatgatcagg tccgagcagt gactcgtaaa tttggattgc gcgatgcacc tcgcgagcgg
    14521 tccatggact cgtgatcaaa gcaaaattaa aaggcagatg gactccggca ccatagtagg
    14581 caacgatacg ctccaaagaa agataggctt cgccgatgat tacgcaatct tcgtattgat
    14641 cgacgactcg ccgcatgcgg cggaggacgg ctgcggtatc gggatgatct ctggagtgga
    14701 ccggcagata acgaaaatac gggcccgcgg ccggatccca agttaggttc aacggattgt
    14761 ctcgaaactc ggggtctttc atcaagtgag cgatcgaatc tacgcggaaa ccgttgacgc
    14821 cgcgatcaag ccaaaatctc agcacatcga gcatcgattt ctgtagagca ggattgcgcc
    14881 agttcagatc cggctgctct ttcaaatagg agtgcaaata atattgcccg ctgttttcat
    14941 cccattccca agcgctccca ccaaatgcgc tcaaccagtt gttgggcggg ccgccgcccg
    15001 gcgctggatc gcgccagata taccagtcgc gaaaggggtt gtttcgagca gatctcgact
    15061 cgagaaacca gaagtgttgg tccgaactgt ggttggggac gtaatccaac aaaactttta
    15121 agcctaaacg atgcgcttca ctgatcagcg cgtcgaaatc accgaggctg ccaaacagcg
    15181 gatgaatttc ggtgaaatcc gatatgtcat aaccaaaatc aaccatgggc gaaggataga
    15241 tcggcgacag ccagaccgca tcgacgccga gccactggca gtaatcaagc cggctgataa
    15301 ttccccgcag gtcgccgctg ccgtcgccat tactgtccat gaatgacagc ggatagattt
    15361 ggtagattaa acccttttgc caccaaaggg cgtcggcgga atgacctgca gctgaaacaa
    15421 ccggggcgga gggaatcatc tcatgcggtt atctatcatc acgtatgcgc aatcgcgcgc
    15481 aaacctttcg ctccgttcgc cgcgactttt accggtgctt tactgtccga agtgatgccg
    15541 gctttcagca gcttcagtac agccgcctct tccggctcga ggccatgtga gtcggtgatc
    15601 tctttcttga gtcgccggcg taaagcgcgc aacaacgagc cgtccgtata ggactgaata
    15661 accgccggat gaatatagca tttacggcaa accgctttgg tattgcccaa cttcttggca
    15721 acgccttcga tggcctgaac tatattgcgt ttgctttcgg aatcagaagc agcggtttcg
    15781 aatttttgca acgcttgcgc cgccaacacc gtccccgccc aggtgcggaa atccttggtg
    15841 ctaaattctt cgccggcaac ttgccggatg tactcgttaa catcgctgga atctatagtg
    15901 cgccgctcgc catcatcgtc gacgtattga aacagctcgt aacccggcag atcctgacaa
    15961 cgcttgatga tcttggccaa gcgcttgtca ctgatatcga gcgcatgttg gacgccgctc
    16021 ttgccgcgaa actcaaaacg aattgtcgaa cccgagacat tgacgtgacg attgcgcagg
    16081 gttgccaatc cgaacgactt gttttgccgg gcgtactcct cattgccgac acggatgtga
    16141 ctggtttcga gcagtctcac cacggttgcg aggattttat cacgaggcaa tccgggtaaa
    16201 cgcaggtcgt tgtcaactct ttggcgaatg agcggcaacg ctttggcaaa cgctagaata
    16261 cgaccgtatt tgttttcatc ccggacctcg cgccagcgag ggtgataacg atgctgtttt
    16321 ctgcccttgg catctcgccc ggtggcttgg aggtggccat taggaagcgg acagatccaa
    16381 acctcacgcc atgccggcgg tatcgccaaa gatttaatgc gttgcagttc tcccggatca
    16441 cgaatgactt tgcctgacgg atcgatatac tggaatgccg cgccacgcct ttggcgtgaa
    16501 atgccgggct tatcatcggt tacatagcgc aggctggcag ccttggccga ctcgagtgga
    16561 tccggcgaca cgatcgcgcc gagccgcttt cccgccggct tccgggttaa ggcgagatcg
    16621 gccttcattg tgttcgagac tttaaaagag aacccgcgta gtcggctcta agaggagagg
    16681 acgcgtgatc acagcgatga tggttattgt gcatggggtc agagttgatt cgagtgtcag
    16741 cggctcggcc cctgagctgg gccgagccgc tactgttcgc gcgggctcag ctgctgtagc
    16801 gcgaatagcc atcgccgcga tcactttgat catggccctc gagcttcacc agcttgttat
    16861 aaagaccgat gatcaaccag gtgggaaccc actgtccgat gaagttcccc cacttgcccc
    16921 gaccgccgat ctgcatcgcc agtgatagcg ccattgcccc aatcgcaact cctaagaacg
    16981 ccgaagatgg tatcgcgccg gtatatttct ctaccgattt ggtaaactga tcctctgtcc
    17041 tgctgggctc ttgtgattgc cctccataag ctccttcata attatccatg aataaccctc
    17101 cttcaatttg atgacggttt agcgaacgat aactcgaaac gtttatttct tctcagcgca
    17161 gaacatttta ggagagcaaa acacatacca gcaccaaatc tcgatcgcga cattgatggt
    17221 cttcagattt ttccaacttg gacaaccggc cgatcttacg atcgaggcag ctcgaagacc
    17281 gtagtcttaa ctctgagacg atggttcaat tttccgtggt atgaagaaac ggagagtgtg
    17341 catttaagtc gatgacggaa ccggccagag gaaagagcct tccgcgcgat ctggactcag
    17401 tcaacgcgct ggggcatcgt cgcagtggca gagggcgaac tgtggcggcg atctcttgaa
    17461 gacgctaatt tgattctaaa aggagcgcct cgatcacgta accacacgat cgcctcgcca
    17521 gcgttcgctg gaattgcggg gatctcaagc attgcgtcgc gcgacaatcc tgcaagttgg
    17581 ctgaaggcgg tcgctccgga tggatcgaga gccaatacaa tgttcaccgc tgaccagagg
    17641 gagcgcgcga cagcggcggg attccccgtc acgcacatca ttccttcaaa ctgttgagga
    17701 atatcgagcg gcgccggctg ccatgatcta gataatacat cctgagcttc atcgacaatg
    17761 atccaatgag gatggccggt cctggcgcga agctgttgaa tgcgcgcaaa cagctcttga
    17821 ataaaagagg ttcgctggcg cacggctaca ccactcaaat taatgatggc gttctccgcc
    17881 ggatccttca gcaaacggat gatttcgtcc actgtaggcg ctcctttatg acggccaagc
    17941 gcgattcccc cgggcacccc ctcaaaagcg ccgcgcggat cgaagaggca gaactgataa
    18001 ccttgccggc aaagactctc gataaagtgg gccgcgacgt ttcgtctctc ggactcggat
    18061 gaaccggcga gcaaaatgtt tgacgcgaac ggcgaaagcc gtacttgcct gccatcgacc
    18121 gaagtgccca gcagcaaatg atgccgggtg agggccgtat cacggtcacg aagatcgttc
    18181 gcaatgattt cattgattag ttctgtgaca ccttggccat tcgctcccgt ggtcacgaaa
    18241 tccgcttttt cctgcaacat gggcagcgcg ttcgcgacgg caacagaaca ttcgcaaagc
    18301 gccaaaagag cgtgatcgtt ttcggcgtcg ccaattgcca cgacgttgtg cggcgacaaa
    18361 cccagttgtt ttagagctgc ggtaagtccg gtggccttgc ttgtccccga aggcaggatc
    18421 atcaccgccc ctttgttgaa gatcaactgc agctccaaac caagctcgcg aatggtgtcg
    18481 aggaccaagc tctcatgtgg atgataggtc gagagaatga cctcgccgac tgaaatcggc
    18541 acgccgcgtt cgcgtagacg ggccatgaac ttcgccggcg gcggactcgc caacagcttt
    18601 ttctcatcgg ttgcggggtg atacagcagg accccatttt cggccactat ccactcgaag
    18661 atatcccaac gaggaaatac cgaaaaaaga tcgtccaatt gacggccggt gacgaggatg
    18721 agtttgcggc ccgaggctaa taaccgttgc agggccgcga gtgtgccgct ggacacttga
    18781 ccatcggtag ccagagttcc atcgtagtca caggccaacg caatataccg catgtttcct
    18841 tcccgtccgc gcatcggatt gcatcctcta agcatagggg aagcccggaa gcatttcaaa
    18901 caacggcttc gccgatctgc cggcatttgc caaggattgg ctggggcttt aaacttacta
    18961 cctactcaac gaaggatctg aatcatgaac gaagtaatcc gcttgcccga cgagttctac
    19021 attcttgcaa ccgcatcggc cgcggaagag tcccgtgtcc taaagatggg cgaaagcttt
    19081 gggattttcg accgatacgg cgatattctt caatatggct taggcgaaca aggaatctat
    19141 catgagggaa cacggttcct ttcctatctt gaactgcgct taggtaacat tcggcctttc
    19201 ctgctgagct cgatgatcaa agaagacaac atccttttta ccgtcgatct gaccaatccc
    19261 gatgtttacg tcgacggcac catagcgttg cgccgtggcg atgtgcatct gtatcgctcc
    19321 aaattcattt ggcaggccat ttgttatgag cggctggaag tggtcaacta tagcttgtcg
    19381 cctatcgaga tttcttttgc gattcgtttc gatgcggatt tcgccgacat ttttgaagtg
    19441 cgcggcgtca aacgagaaaa gcgcgggacg cgcctggagg atcagatcga tgccaagcgg
    19501 gttgtcctga gctatctcgg tctggatgac atcgttcggc aaacgatcat tgagttttcc
    19561 ccagctccgg ataagctggg gccgtccgaa gcgtcctttc acagagtgct caatccgaaa
    19621 aaacacgagg cgtatttagt ctcttacgcc tgcaaatcca atcgctcagc tccggttcga
    19681 cgtcctgtcg agctggcatt gcgagaagcc gagcgagcgc taaccgcata caaggcggag
    19741 gaatgccaga tccgcacgtc gaatcatgag ttcaatggat ggctcgaccg ctcctatgcc
    19801 gatattcaca tgatgattac cgacagccaa ggtttctatc cctacgcagg agtgccgtgg
    19861 ttcagtacgg tcttcggccg ggacgggatt ctaacatcct gggagctgtt atggcttttc
    19921 ccacgagtag cgcgcggtgt gctgagttac ctggcagaaa accaagcgac cgaagttatc
    19981 tccgaacgag acgcggagcc tggaaagatt ttgcacgaaa ctcgccgcgg agaaatggcc
    20041 aatttgggtg aaattccttt cgatcgttac tacggcagca ccgacagcac accgttgttt
    20101 atcatgctgg cgggcgctta cgtcgcccgc accaacgatg ctgaatacgc gcgctggatc
    20161 tggccgcacg tcgagcgcgc gctacactgg atcgaaaaat acggcgatac cgacggagat
    20221 ggctttgtcg aaactttccg ccgctcatcc catggtttgg tgcagcaagg atggaaagac
    20281 tcgtgggatt cggttttcca tcgagacgga acgctggccg cagcgccgct ggcgttgtgt
    20341 gaggtccagg gttacgtcta cgccgccaaa cacgctgcgg cagagatcgc gacgcttctc
    20401 ggacatagcg aggaagcgct caggttggct agcgcggccg agcgactcaa agaacaattc
    20461 aaccaaatgt tctggtgtga cgagctttca acctatgcgt tggccctcga ccgagacaag
    20521 cgtccttgca gggtgagagc atcaaacgcc ggccattgtc tgttcgccgg cattgccact
    20581 gatgagtacg cccggcgtac tgcgaaaacg ttgatgagcg aagattcttt ttccggctgg
    20641 ggaatccgga cgttgtcagc cggcgaagtg cgctacaatc ccatgtctta ccacaatgga
    20701 tcggtatggc cgcatgacaa tgcggtgatc gccgccggct ttgcccgtta tggtttgaag
    20761 gagccgattt ctcgagtaat caccggatta ttcgaagcga gtctctatct tgacatgcat
    20821 cgtttgcccg agcttttctg tggatttcca agacgcgaag gtgaagggcc gactctctac
    20881 ccggttgcct gcgcgcctca ggcctgggct gcgggctcgg tgttcctgat gctgcaagct
    20941 tgtttggggc ttttggttca tgcgagcgag ggcaaggttc acttcgactc gcctgagctg
    21001 cccgagtgca tcgatactat acatttgaaa aacctgcaga tcgattcgtc acttctcgat
    21061 ctggtagtcg atcgtagttt ccaaggtatc ggagttgagc gccgccaggg caatgcgagc
    21121 attgtgatca gctgaagctt tgatgacccg gcgatagacg ctcaggtagt cgtgcgccat
    21181 gcgagtggcg ctaaaacgtt cttcgaaaac ctggcggcag cgccggcggt caaaagatgg
    21241 aatcttacca agcgcctcaa gagcgccctc gatgtcatcg actatgtaac ccgtgattcc
    21301 ctcctggatc agctccggaa ccgaaccaca gcgataagcg ataatcggag tgccgcatgc
    21361 catcgcctcg atgatgacca ggccgaacgg ttccggccaa tcgatcggaa acagcaaggc
    21421 ataagcgttg gcgagaaact ctttcttgtc cttgtccccg atttccccaa ttaactccgc
    21481 cgacgaagca ctcaggatcg gtctaatcac ctgctccatg tattcgcgat cggcagcatc
    21541 ggttttggcg gcaatacgca atttcatgcc cgaacgagcg gcgatctcga ttgcgcgatc
    21601 cagtcgtttt tccggtgaga ttcgtccgat gaatgcaaga taatccccag gtttttcact
    21661 gcattcataa agcccgagag gcaagccatg atagatcgtg tcttgccagt tagcgcgcgg
    21721 gaggggtttg cgctgcgcgt tcgaaatcga caccaatggc atttcactga atttcttgta
    21781 gaccggcatc aggtcgggaa gatcgagcct gccatgaagg gtcgtcacat gggcagccga
    21841 gtgtcgccgc gccagcggga aatgcaggta atccgtatga aaatgaatga tgtcgaagcg
    21901 cgccgccctc tcgaaaacct cctctagcat cacgtaatgg tgagcaagcg gatcgaagac
    21961 ttcgtcaagc cgtaaggcgc gcttgcgggc cgcgattagt tgcgcggcag tgaccgaatc
    22021 gccgcttgcg aagagtgtga cttcgtgtcc ctgcgccacc aactcctcgg tcaggtacga
    22081 aacgatccgc tcagtgccgc cgtaggtttg cggcggcaca ctttcataga ggggcgagac
    22141 ttgggcaatt ctcattaatt tttcctgcac agaatgccag gagcaaaatc aaaccggctg
    22201 aagcaaagcc aactgttgcg tgggtaactc ccagcaagag caagcgcggt gccaggaaag
    22261 acttgttcgt tgtgccgcaa tttcagccag cagaattgat gatgatggca agttccagca
    22321 acaccgccgg cctgttagtc gtgcgcttac ggcacattgt aactatgtag ttgcttcagt
    22381 cttgggacgt acaactcttc gcggacaatt aagcttcagg ggctcattcc tcttttcctt
    22441 ttccgctacg cctggcaaat ttctgtcgcc gactgcggtg cttgctgctg tcgggtcgtg
    22501 cgcgcgcccg tactgttcgt cgcttgaaga tcaacagttg gcgagtctga ttatgtggat
    22561 tccaggcggg gtcatcatgg cgagtacccc tatcgtgctc tttggcctat ggcttaaaga
    22621 aagcgaccgg cggatgtttg cccgcgagcg tctggcactt gcacgatcag tggcggtgat
    22681 ggatcggcat gaaggtgaag atgaaagcac cgcagttctg gatcgcatta ttgcggattg
    22741 tcgttggcgc atggttcgtc aaagcggtat ggaccaagct gagcctggct attgcgtggc
    22801 gtattattcc atatccggcg gttagccagc gcttcatagg ctttcatccg aagcgcgtcg
    22861 ccgactttgc agcgggagat ccggtcgagc cctacaagca gtttatccag gagaccgttt
    22921 tacccaacgc gcaattattc gcccggcttc aggcttatgg tgaagtggtg gtcggctttg
    22981 gcttgctcac gggtctttgt gtcggaatca cggccttgat ctgtcttctt ctctgcatca
    23041 attatgggtt ggctactcat atgggttggc tactcagtgg atgagtttcg gccagcaagg
    23101 ctttcatctt ttgctgatca cctcgatgct gatttttctc ggcgcgcgcg ggacgctcat
    23161 ggggtctaga tggtttgatt ttgcgcgtag cggcgccgtt ccggcgtccc tggctaaaaa
    23221 ttattatgtc gctcgcggtt ctcgctctgt tgcctgccgg gaccaaagct ggcgcggcgg
    23281 aattgcgcgt gttcgtaacc aacgagaagt caaacaacgt caccgtgatt gaagctctat
    23341 cgggcaaggt tctggcgacc attgcggtgg ggcaggggcc ccgcggtgtc gctaacagca
    23401 ccgagggcgg gcgcgtgttt gtcgccaata gtaacagcaa caacgtcagt gtcatcgata
    23461 cggcgagcct gaaagtgatc gactcgtttc cggccggtac cgatccggaa gggatcaccg
    23521 tcgatggccg caaccggctg tatgtcgcca atgagaatga gtcggcgctg acgattatcg
    23581 atatttccaa acgccaagtc gtcaagcgtc atgaagtcgg tctcgaaccg gagaccgcgg
    23641 tgctttcccc tgacgaacgg tgggtgaccg tttccaacga atcttccagt gatttttact
    23701 tcatcaatgc cgagcctgcg gcgatggctg gcaaagtcgg tgtgccgcgc aatccccgcg
    23761 gcatgcgcct cagcgccgat tcgcgccggc tctacgttgc cagcgagcag gaaccgatgg
    23821 tctcgatcat cgatgtcgcc caacgcaagc tgatcgggtc gatgccgacc ggcggtgagc
    23881 ggccggtgga tattgttatc tgcccaaacg gccaacgcat ctacgtctcg catgggcgat
    23941 cggaagatgt ccgcgtgttc gacgccgcca cttggaagct actggccacc atcccagtgg
    24001 gaccgcgtgc ctggtggatg acccagacac cggacggaaa gtttctttat gtcacggtcg
    24061 gccgcgccaa tgaggttgtg atgatcgata cccgttcgaa caccatcgcg cgtcgcattc
    24121 ccgtcggtca attgccgtgg ggcattgcta tcgccgaggt gaaatgatca gaacctggat
    24181 ttcagccgcc agcttggaac gatatggacg ggctccaagg cgactacctt gcaaaaacag
    24241 gttagcaatg ctccggaggg tcacaggttc atctcaaacc gttcacttga tgaactgatc
    24301 tgagtcacaa aaggatcacg caatgtgatg cttcatttag aacacatgga cgatgaacac
    24361 ccgaagcgcc tacgagacat ttacgaaagg ttaggcaatc aggcacgggc cgagcgtaaa
    24421 gatagaagct gatgagctgt tttccaagca gtgcagggtc tctttgcagg gcaatcatgc
    24481 caatgtccga ggaagacgtt tgattgcctg cctgaaatac cgccgcgtca aagatgcgct
    24541 ctaaaatagc tcttgcaaag aatagagctt ttgatacatt tcactccccc ggctaagggt
    24601 ttcaaccagg aggtttactc caatggtatg ggacgtgatg gttgcagcgg cattcgttgt
    24661 tgtatctgtg gcggtccttg cttgtttcgg attcaagttg cttctcacct ggttaaagaa
    24721 gcctctccaa aatactccgc aacaccgcct gtcggtctca tcacgctatg gacaattgcg
    24781 tcaacgtcta agtcaaactc acgaaggtga gaggttaagg aacaatccga cgatgaagaa
    24841 gaaattgtca gcggaactgg aaagactcga gatcgagcac ggtcacgaat ggcgcgggat
    24901 aatccaagaa cagttcggtc ggctgtggct cagattaaac atgccggaaa agaaaccggc
    24961 aaaggaacca ttaacattac caaagattag gggtgagggc ggcaaggtcg cataacgcat
    25021 gaacattggg tccaagttgt cgagcgcctg gctctatgcc gggtgcctct cgcgctcgac
    25081 ttattctctc gtggctctta gggagagcgt cataagccgg gagatgacta gacagttgcg
    25141 ccaagtcatg tgtcgaatga gaggccataa gctgaaggta atagttgagg acatggtatc
    25201 ggtctgcgaa cggtgcgagg ctacttacct acggaaggat ctcgccaagc cgcgtcgtgg
    25261 gcaggtctaa ccatacgccg gccattctcg cgcgcttccg gccgtaacac gatttcgggg
    25321 tttgccgtct gtcgctttgt ttcgcttatg ctgaaggtga tccgctcagg gcctgccagg
    25381 actcatcaca agtcccgcag ttgtttcgca caaattcgaa aactcgtaga aaaccgcacc
    25441 tgccggaatg ttcgcccctt aatactaaat tgctggaagc aactctggga caatagttta
    25501 acctgagtcg tcgcgccacg gaatggtgtc tttgttcatg gccagcacgg cccctaaatc
    25561 acctcatccc catcgtctgt ctcgcggcaa gtagtttatt tcagcgccga gtgggattcg
    25621 cctcgatacc gagccggtta agcagccatc ttctcagccg atcatcctga gcaatttcgc
    25681 tcccggtttg ttctcaggat gatcccggct ttgtttgccc tgataaactg ttacgctgaa
    25741 ttattagcag attacggcgt cgggctagtt tcatcgcttt acgccgtaca cagcggactg
    25801 tgcaggttga gttccatcca aggagaagca catgcgaaaa cgcatcacca atcatgatgc
    25861 tcaaaaatac gcggctgtgg atgaaggctg gcttgattta gaaaaggtgg cacaggttga
    25921 agtatcctct gaagaagcgc agcacccgat cgaagcggcg ctgttgccgg gggtttcggc
    25981 cggttggcgc gcggcagcgc cgggacaaca gctagttcgt ctgatgttcg acaaaccgca
    26041 gaatctccgg ctgattcatc tggtgttcga ggagcatggc caggaacggc tgcaggagta
    26101 tacgttgcgc tggtcctcgg gtgtcggcca ggcttatcgc gagatcgtgc gacagcaata
    26161 caactttagc cctccgacaa ccaccaccga ggtggaaaat cacacggtta atctcgacag
    26221 ttgcgcggaa ttggagctta gtattattcc cgatctcaga ggatcggcag ctcacgccgc
    26281 ggttgtggag tttcgcgtgg cttaaacaat gatgctgccc ctgaacggtt attaattaaa
    26341 ggagattgca atgattgaag ccaacagcaa gtgtccaaaa tgcggcgccg gcatcatgga
    26401 aatagttccg gagcacagag ccattgcgcc ggatctgccc gaggataacg aactcaaggc
    26461 aaggtgcgac aaatgcggct acgaggatca ggtcgaagtg gtagcgaccg atgagtaagg
    26521 cgaaatggaa aggcgttcga acattcgtta tcatcgttct gtgaattagc gcatccttcc
    26581 tcttggcgct tcgtattgtt accccttctg acggtctgat aaatagcgaa cgcgtcgcga
    26641 aattattaat gtcagacctg agccgagcct gcctcgtcaa ctatgcgaaa gttcgagagg
    26701 cctggctgaa tgccacaaac ggatcaacgt gctctcaccc atgccgatcc ccgtatcacg
    26761 aaccttgaga acagcggtcc ttgatcgatg tctaggctca cgcgtggccg aggctgaaga
    26821 gatccgtcaa tcggtttctg cgactcgggc tttcaccaca acactatcaa agtgattcat
    26881 ttgaaatgca cggtggctcg tgctctgtga catcgcgcta ccttggatgg atcgccacgc
    26941 gatagcacgg aagatccggc aagatccgga gctcaaagat gtctatctga ttgcgatctc
    27001 gggattcgga caggacgaag accagcaact agccagagac gccggattta acgagtaccg
    27061 cgtcaagccg gtggatctaa gatccctcga ccagctgata aagcatcgcc aaggtcccag
    27121 ccggcgctag gcgcattttg aagttcactg ctcctttgaa tgcccggtgt cttctgactc
    27181 tcgcttccag ttatgcgaca agcttgcgga tctttttagg ttttccgctt t
//
LOCUS       DASUVS010000361         4106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1899653, whole genome shotgun sequence.
ACCESSION   DASUVS010000361 DASUVS010000000
VERSION     DASUVS010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1899653"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VF182_19310"
     CDS             complement(<1..1259)
                     /locus_tag="VF182_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX6439277.1"
                     /translation="MEREEKDVAQSPKPENDRGNGKGLKSERHKWVASLEYILEYILK
                     NDESEQAGHLLDELADRLRESGLKVPDRISTPYLNTIRAEKEPKYPGNREIERRIKSY
                     VRWNAMAMVVKANRLHADIGGHISTYASSATLYEVGLNHFFRGSDGDNNADMIYFQGH
                     ASPGVYSRAYVEWRINAPKLHHFRQELSSVGGISSYPHPYLMPNFWQFPSVSMGLAPI
                     MSIYQARFNRYLKSRGLLSGEEPRVWCFPGDGEMDEPEASGALTVASRENLDNLIWVV
                     NCNLQRLDGPVRGNGKIIQELESLFRGAGWNVIKVIWGSNWDPLLEADNDGLLLRRME
                     EAVDGDYQKYSVEPGSYTRKHFFGKYPELLERVNHLTDDQIRKLLRGGHDPAKVYAAY
                     KAAVEHKGAPTVILAKTIKGYGLGEAG"
     gene            1316..2035
                     /locus_tag="VF182_19315"
     CDS             1316..2035
                     /locus_tag="VF182_19315"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEX6439278.1"
                     /translation="MQYIELTLRDPASNLACDEALIELFEARCATDGLLRIWEPENHF
                     VVLGHSNRIDSEVDFAACESDRVSILRRLSGGGTVMQGPGCLNYSLILRNGIDIPEGI
                     SESFQFVLEHHRQCIQQLIEDSVEIAGISDLVVNGQKISGNAQYRKRLFTLVHGTFLL
                     NLDLAIVARCLRLPLKQPVYRQNRSHSCFMRNLHLNPINVREAIKSVWSASDPYMYVP
                     HAMIAHLKKSRYDRPGWTRKF"
     gene            complement(2079..2155)
                     /locus_tag="VF182_19320"
     tRNA            complement(2079..2155)
                     /locus_tag="VF182_19320"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2119..2121),aa:Met,seq:cat)
     gene            complement(2191..2919)
                     /locus_tag="VF182_19325"
     CDS             complement(2191..2919)
                     /locus_tag="VF182_19325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX6439279.1"
                     /translation="MSCFVIGDIHGCLAELRYLIDQLPIEHGDRLVFLGDYIDRGPDS
                     MGVVSYLLELPKQATVDAVFLKGNHEDMFLDYLGLSGNHGEVFLSNGGLATLASYGIG
                     APTLGGLEIAEQLPSAHLNFYQSLETFYVVKPYICVHAGIQPLKKLEEQTATEMYWIR
                     AEFIRNPHKLPFTVLFGHTPQKEVLFDLPYKIGLDTGLVYGNKLSCLAIEEKALYQIK
                     RGNKTIQKVPVHDNWSKKLKFHAP"
     gene            3016..3390
                     /locus_tag="VF182_19330"
     CDS             3016..3390
                     /locus_tag="VF182_19330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439280.1"
                     /translation="MKIRDVYSSYSLILGIVCLFLGAGNWIVGILESAKYQALLHKTT
                     QTGLEESYRSFQELDQQKNEEVLLRINQDRERYNAARVKLDFYHVVLIGGRWLLLIGA
                     GVTVASIIRLIRRDVPVSMTTT"
     gene            complement(3399..>4106)
                     /locus_tag="VF182_19335"
     CDS             complement(3399..>4106)
                     /locus_tag="VF182_19335"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX6439281.1"
                     /translation="GTELKVEALNAPHLTGLGNALANLHLIGRGYKKGIENRFGFNRI
                     VMTYREARRQLPPHLKHIIRVLDDEFSYLENYLDNNLPKGIIHGDLFPDNVKFKGSRL
                     VGVLDFEAACRGKLIYDLATAVNALCFVDGRYRIDRFEALISGYESLRPLSLPEWDSF
                     PNELRFSALRFTVTRIKDFFLRKVDENQRVYKDFKEFYERLLILRREKTGGMEDILLA
                     MATGYDYRKYQKSKPSK"
BASE COUNT         1020 a         1020 c         1005 g         1061 t
ORIGIN      
        1 tctcctgcct cgcccaaacc gtaacccttt atagttttgg ccaaaataac cgtcggtgcg
       61 cctttgtgtt cgaccgctgc cttataggcc gcgtaaactt ttgccgggtc atgaccgccg
      121 cgcagcagtt tgcgtatttg atcgtcggta aggtggttga ctctctccag aagctcggga
      181 tatttgccaa agaagtgttt gcgtgtgtag ctgcccggtt cgactgaata tttctgataa
      241 tctccgtcga ccgcctcttc cattctcctg agaagtaatc catcgttatc cgcttcaagt
      301 aacgggtccc aattagatcc ccaaatgact ttgatcacgt tccagccggc gccacgaaag
      361 agcgattcga gctcctgaat gatttttcca ttgccgcgca caggaccgtc gagccgttgc
      421 agattgcagt tcacaaccca gatcaaatta tccagattct cgcgagaggc cactgtgagg
      481 gctccggagg cttccggttc atccatctca ccatcgccgg gaaaacacca aacccgtggc
      541 tcttcaccgc tcagtaagcc ccggcttttt aaataccgat taaagcgcgc ctgataaatt
      601 gacataatcg gcgccaaccc cattgatact gatggaaact gccaaaaatt cggcatcaga
      661 tacggatgag gatacgatga gataccgccg acgctcgaca gctcttgtct gaagtggtga
      721 agcttaggag cattgatccg ccactcgaca taagcgcgcg aatacacgcc gggagaagcg
      781 tggccttgga aatagatcat gtccgcattg ttatcgccgt cgcttcctct gaaaaaatgg
      841 ttcagcccaa cctcatagag agttgccgac gacgcatacg tcgaaatatg cccgccgata
      901 tcggcgtgaa gtcgattggc tttcaccacc atcgccatgg cgttccaacg cacatagctc
      961 ttgatgcgcc tttcgatctc gcgattgcct ggatacttcg gctctttctc tgcacggatg
     1021 gtgttaaggt agggtgtgct gatcctatca ggaaccttca gtcctgattc gcgcagacga
     1081 tccgcaagct cgtcgagaag gtgacctgct tgttccgatt cgtcgttctt tagaatatat
     1141 tcgagtatgt actccagaga agcaacccac ttgtgtcgtt cgcttttaag ccctttgccg
     1201 tttccgcggt cgttttcagg tttgggactt tgagccacgt ctttttcttc tcgctccatg
     1261 ggagtctcct aggtttttag cagacaacaa aacatcactg aaatcaacta accgtatgca
     1321 atatatcgaa cttactctgc gcgatccagc aagcaatctc gcctgcgacg aggcgttgat
     1381 tgagctgttc gaagctcggt gcgcaacgga tggtcttctg agaatctggg aaccggaaaa
     1441 ccatttcgtt gtgttgggtc attcgaatcg gatcgattcc gaagtggact tcgcagcctg
     1501 tgaatccgat cgagtttcaa tcttgcgccg tttaagcggg ggcggcactg ttatgcaggg
     1561 acctggttgt ctgaactaca gtttgatttt acgaaatggc atcgacattc ctgagggcat
     1621 ttcagaatcg ttccagtttg tgttggaaca tcataggcaa tgcatccaac aattgataga
     1681 ggattcggta gaaattgccg gcatcagtga tcttgtcgtg aacggacaga aaatctctgg
     1741 caatgcgcaa taccgcaaac gtttgtttac tttggtgcac gggacttttt tgctcaacct
     1801 agatcttgca attgtcgctc gctgcctgcg gctcccgttg aaacagccgg tctatcgtca
     1861 gaaccgatcg catagctgtt tcatgcgcaa cttacattta aatccgataa acgtgcgtga
     1921 agcgatcaaa tcggtatggt ccgcgagcga tccatacatg tatgtgcctc acgctatgat
     1981 cgctcatctt aagaaaagcc gctatgaccg gccaggctgg acgcggaagt tctagatgat
     2041 gcgcaacgga aaattcgccg atggtagcgg tgaaagactg gtggcggtgg gcggagtcga
     2101 accgccgacg ccgcggatat gagccgcgtg ctctgaccaa ctgagctaca ccgccgtgga
     2161 attctaaatt aagctgggat gagggaaaga tcaaggagca tggaacttga gctttttgct
     2221 ccagttgtcg tggaccggga ccttctgaat cgttttattt ccccgcttga tttgatagag
     2281 cgctttctct tcgatcgcaa gacagctcaa tttattgcca tagacaagcc ccgtatctaa
     2341 tccgattttg taaggaagat cgaacagaac ctctttttgt ggagtgtggc cgaagaggac
     2401 cgtgaaagga agtttgtgag gattgcgaat aaactcggcc ctaatccaat acatttctgt
     2461 tgcggtttgt tcttctaatt ttttcagagg ttgaattccg gcgtgaacac aaatataggg
     2521 cttcaccacg tagaaggtct caagactttg ataaaaattt aagtgcgccg atggaagttg
     2581 ctcggcgatt tcaagaccgc ccaaggtcgg tgcgccgatc ccgtaacttg cgagagtagc
     2641 gaggccgcca ttacttaaaa aaacttctcc atggttcccc gataagccga ggtaatccaa
     2701 aaacatatcc tcgtggttcc ccttaagaaa aactgcatct acggtcgctt gtttcggaag
     2761 ttccaagagg taagaaacca cacccatgga atcgggaccc cggtcgatat aatcacccag
     2821 aaataccagg cgatcgccgt gctcaatcgg caactggtca attaggtacc ggagctcagc
     2881 aagacaaccg tgtatatctc caattacaaa acaactcatg acctatggta gttcacgggg
     2941 aaccatactt caaactgcgc ttacgcggct tacacaaaaa ttgacgcgta tagtggtatt
     3001 tgataagaga acccaatgaa gatccgtgac gtttactcta gctactcctt gattttgggt
     3061 atcgtgtgcc tctttctcgg tgctggaaat tggattgttg gaattctgga aagcgcgaaa
     3121 taccaagcgc tgttgcacaa aaccacgcag accggcctgg aggagtccta ccgcagcttt
     3181 caagaactcg atcaacagaa aaatgaagag gttttactac gaatcaacca agacagggag
     3241 agatataacg ccgcgcgtgt caaactagat ttttaccatg tcgttctgat tggcgggcgt
     3301 tggctgttgc tgattggcgc tggcgtaact gtcgcatcga ttatacgctt gatccgccgt
     3361 gatgttcccg tgagtatgac tacgacttaa cgctagcgct atttggaggg tttggatttc
     3421 tgatatttac gataatcgta cccggtagcc atggcgagca ggatatcctc cattccaccg
     3481 gttttctcgc gtcgtaatat cagcaatcgt tcataaaatt ctttgaaatc tttatatact
     3541 ctttgatttt cgtctacctt gcgtagaaaa aaatccttga tacgagtcac cgtaaagcgc
     3601 agtgctgaga agcgtagttc attaggaaac gaatcccatt ctggaagtga caagggccgc
     3661 aagctttcgt agccagaaat caacgcctcg aaacggtcga ttcggtaccg gccgtcgaca
     3721 aaacagaggg cgttaaccgc cgtagccaga tcgtagatga gcttgcctcg gcaggctgcc
     3781 tcaaaatcca ggacgccaac caatcgacta cccttgaact taacattgtc gggaaaaaga
     3841 tctccatgga tgatgccttt gggcaaattg ttgtccaggt aattctcgag ataagagaac
     3901 tcatcgtcta gaacccggat gatatgcttc aggtgaggcg gtagttgacg ccgcgcctct
     3961 cgataagtca tgacaatccg gttgaaacca aaccgattct ctattccttt cttgtaaccg
     4021 cgaccgataa ggtgaagatt ggctaaagca ttgcctaggc cggtcaaatg cggcgcattc
     4081 agtgcttcga ctttgagttc tgtgcc
//
LOCUS       DASUVS010000362        20603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1463457, whole genome shotgun sequence.
ACCESSION   DASUVS010000362 DASUVS010000000
VERSION     DASUVS010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 20603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20603
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463457"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="VF182_19340"
     CDS             complement(<1..1389)
                     /locus_tag="VF182_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019872798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEX6439282.1"
                     /translation="MAEEKYETHDYDVVIIGAGGAGLRAAVEASAQGARTALVCKSLL
                     GKAHTVMAEGGIAAAMGNVYPEDNWKVHFRDTMRGGKLLNNWRMAQIHAQEAPDRVLE
                     LEEWGALFDRTHDGLILQRDFGGHRYARLAHVGDRTGLEMIRTLQQHAVHKGIDVYME
                     CNLQRLLTNGGKISGAFGYWRETGKFIVFKCKAVVLATGGGGKAWKVTSNSWEYTGDG
                     VTMALDVGADLMDMEFMQFHPTGMVWPPSVRGILVTEGVRGDGGTLKNTKNERFMFNY
                     IPEFFRAETAETEKEADAWYEDKKNNRRTPDLLPRDEVARAINSEVKAGRGTPHGGVF
                     LDIASRRPTDYIQRRLPSMYHQFKELADVDITKEPMEVGPTCHYSMGGVRVDADSTAT
                     TVPGLFAAGEVAAGLHGANRLGGNSLSDLLVFGRRAGLYAAEYAKTVKGAVDVDAGQI
                     DHYAQELVSPFES"
     gene            complement(1389..2153)
                     /locus_tag="VF182_19345"
     CDS             complement(1389..2153)
                     /locus_tag="VF182_19345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439283.1"
                     /translation="MSNKTLTLDQFRTLRKDTWWKEPLTVVLVLGAFAIYATWAALQN
                     AHYYVAPYLSPFYSPCIAENCAHVSVPLVGSWWNLSPAFLILWIPGGFRATCYYYRKA
                     YYRSFFLSPPACAVRDASKSYSGETRFPFLIQNLHRYFFYLSILVLAFLWWDAFLAFR
                     FPNGFGIGLGTLVILINAILLTLFSLSCNSCRHVCGGYLDSFHSAPKRYNVWSFISRL
                     NGRHMEYAWISLIWVALTDLYVRLLSMGVIQDLRFF"
     gene            complement(2650..2853)
                     /locus_tag="VF182_19350"
     CDS             complement(2650..2853)
                     /locus_tag="VF182_19350"
                     /inference="COORDINATES: protein motif:HMM:NF021900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439284.1"
                     /translation="MVEKTSVMVTALARWIGYDAAAAGAHEAVATCKSVREVARLKTA
                     LTSEQLEKSSEPWNMTMPGREKH"
     gene            3079..3774
                     /locus_tag="VF182_19355"
     CDS             3079..3774
                     /locus_tag="VF182_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX6439285.1"
                     /translation="MVIDELAKALDASNTRIVQCRRCPRLVRWRETCAKTPPRRYHGV
                     EYWARPLAGFGDPHARLLIVGLAPAANGGNRTGRMFTGDRSGDWLYGALHAFGFANQP
                     TSEHRDDGLELTNCYITAAVHCAPPLNKPALIEFERCRPFLVHELKLMTQVRVVVVLG
                     KIAFDSFLKAYKEAGRKIPNPRPKFGHGIATILPDGVTLICSYHPSQQNTFTGKLTQP
                     MFYKVFDHAKALL"
     gene            4005..6896
                     /locus_tag="VF182_19360"
     CDS             4005..6896
                     /locus_tag="VF182_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6439286.1"
                     /translation="MKNQFKDVAAELRKRLEGEVKFDPYTLLLYSTDASIYQIEPIGV
                     VVPRHKGDVQAAIEVANRFDIPILSRGGGTSLAGQAVGHAIVLDFSKYMRNVLEVNEE
                     ELWCRVQPGLVQDELNAHVRSLGLQFGPDTSTSNRATIGGMIGNNSAGAHSLTYGKTL
                     DHVLELTVLLSDGTEAVLKDLSSGGLAAKMQARGLEGQVYREVSRLAKEHENEIVSRY
                     PKIMRRVSGYNLDEFIKPQPFNLARVITGSEGTLATIIEAKMRLVPKPKWTAMDVIHF
                     HNDQEALECSQLVLQTAPYAMESTDKMILNLARGNIEQSRKLGFIQGQPESLLMVEYA
                     GETQDEVKAQVSKLEDLRAAQRIGYAATQAYKPEEVKAIWGVRKAGLGLLLGTKGDKK
                     PIAFVEDTAVAPAKLPEFIKRFKEIISSHDAIAGYYGHCSVGCMHIRPLINLKEPAEV
                     DKMVSISNAISDLVLEFDGAMSGEHGDGLARSHFNKKLFGPALYESFRQIKRAFDPKN
                     RLNPGKIVDAPAMTESLKIHPKYQTWIPHTTLDFSAQGGFARAVEMCSGMGECRKKLE
                     GTMCPSYMGTLDEEHSTRGRANALRTVLSGKVPKDDFTGKRLHEIMDLCLECKACKAE
                     CPSNVDMAKLKYEFLDHYHRANGLPLRNRLFGGIARLNRIGAQFAPVANGIAGSRLNR
                     WLMEKLAGIDRRRPLPKLAGRTFESWFGNHSGEGDGSKGEAVLFHDTFNDFNTPHVAI
                     AATRLLEHLGFRVLLTNKRCCGRPMISKGMLAEAKNNAVWNVDQLAPFAERGIPIIGL
                     EPSCLLTLRDEYPEFLRTSAAKKVAAQSFLFEEFLASELAAGRLSVKLSGSRGKALLH
                     GHCHQKALVGTAPSIAILRAAGFEVSEVDSGCCGMAGSFGFEKEHYDLSVTIGNRRLA
                     PAVKSAGTDVEIVAPGISCRQQIEHLTGRKAKHPAELLCDTLAPSCEDR"
     gene            complement(7092..7535)
                     /locus_tag="VF182_19365"
     CDS             complement(7092..7535)
                     /locus_tag="VF182_19365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX6439287.1"
                     /translation="MGKLLLAFAMWFALSGLALAAVNINTATTEELTSIKGIGEKRAQ
                     DIINYRTKNGNFKSVDDLEKVPGIGPGLMKQIRPQVTTTGKTVIDQSAEKGAKSKPAD
                     SGKSTKSSDSKKSESAKTESPKADTTKAERAKEKTAEKSKSAETR"
     gene            complement(7684..8118)
                     /locus_tag="VF182_19370"
     CDS             complement(7684..8118)
                     /locus_tag="VF182_19370"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEX6439288.1"
                     /translation="MSARKLANAEQSDVCQLSGDPDEIEQAAASAGLTVIRIDLKGLA
                     SKVGLIGRVARALNFPDWFGKNWDALNDCLTDLSWLDGRGWVVIFENSKHLAARKPQV
                     FHSAVEVFQSVNDYWRQAGKPFWVLFHGPGDWVTGLEKFDGD"
     gene            complement(8115..8432)
                     /locus_tag="VF182_19375"
     CDS             complement(8115..8432)
                     /locus_tag="VF182_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018534820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HEX6439289.1"
                     /translation="MYGQDQPRTRIKEVALQELPSEARTTLQLIEKGGPFPHPREGAV
                     FGNFERRLPLKKRGYYREFTVPTPGRRDRGARRIVAGENGECYYTEDHYRTFREVRKG
                     LSP"
     gene            8467..8643
                     /locus_tag="VF182_19380"
     CDS             8467..8643
                     /locus_tag="VF182_19380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439290.1"
                     /translation="MSRANSLITAGTLIVRYPKVPALKSEQLVRRGIDWITASQMAVI
                     LLSEYIRYPGRVKV"
     gene            complement(8644..10143)
                     /locus_tag="VF182_19385"
     CDS             complement(8644..10143)
                     /locus_tag="VF182_19385"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6439291.1"
                     /translation="MLEAFVSGFWQVVAWPAFGYMLIGIFIGFWVGLLPGIGGLSTLA
                     LMLPFSYSIKDPISAFAFLLGMLAVTGTTGDLTSILFGIPGEGSSAALILDGYPMSKN
                     GEAGRALGAAIMSSVLGSIIGAVSLALSIPIMRPLVLLFGSPELFIIAIMGITFIGTL
                     AGPSLIKGLLAGGIGLMLAAIGVDPQTGSLRYTFGSLYLWDRLDLIPVVVGLFAIPEI
                     VDLAVRGTSIAGGKTGKLTGVMDGVKDTFRHFWLVVRCSLIGVYFGVLPVLGANVAQW
                     ISYAHAVSGLKDKSRAGKGAIEGLLGPGAANNSTRGGDLIPTIAFGIPGSGSMALLLG
                     AMLIVGLNPGPEMLKSRLDVTFSMVWVLIIANIIVAGICFLFLNHLAALTFIRGSLLI
                     PFLMFLVFIGSFTANNDIMDVIFTLIFGLVGYFMVKYGWPRPPLILGLTLGKIAENYL
                     WISTAAYGAKWLLFPTVMGLIALTVFTVAYPTIKERMGKAKYERPEDAV"
     gene            complement(10493..11182)
                     /locus_tag="VF182_19390"
     CDS             complement(10493..11182)
                     /locus_tag="VF182_19390"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6439292.1"
                     /translation="MTTQETLALACRILAMHGHNDFIYGHVSALTDTPGQYWIKGSGI
                     GLEEVTEDDLVLIDFAGNKIAGKRKRHNEFPIHSEVYRTNPEIRCVIHTHPVYSTIIA
                     SSEHRLLPITNMSCAFFPPALKKFDESSDLIVTPEQGVAVARLLGEHKIVLLRNHGIV
                     VAANSIEEACVRGVLLEHSAQTQVAAASIGAFSWSTDAEALLKRKRFYHPDAVHNLWE
                     YFCRQLHRKQG"
     gene            complement(11206..11985)
                     /locus_tag="VF182_19395"
     CDS             complement(11206..11985)
                     /locus_tag="VF182_19395"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6439293.1"
                     /translation="MFKGIDHLVIVVEDLRQAAADYEKLGFTVVPGGKHPVGSHNMLI
                     SFSDGSYLELIGFYRDASDHRWWQPLQTGERLVDYCLQTDDLQGDTQKLRAAGVAIND
                     PVPWSRTRPDGYELKWILSLATGSHRGFAPFLIQDITPRNERIPQQFHHKNGAVGIDR
                     VTIAVNELSEVDRWYSEVLEQSGKPIARTELGAEGLAFQIGPHSMEFLMPYNASSSLV
                     DWIRTYGASPYSATLHSQGAAPETLDNHLLHGARLELTGAN"
     gene            complement(12008..13033)
                     /locus_tag="VF182_19400"
     CDS             complement(12008..13033)
                     /locus_tag="VF182_19400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6439294.1"
                     /translation="MESSKENRIMASFGLTLANRGVIIGAVTVPELFEMAKRGEDSGA
                     FDAVWVGDSLLAKPRLESITLLSALAVVTKKMRLAVGCMATFPQRHPALLAQQWTSLD
                     VISAGRAWLAVCLGGPNEQSPAQALEHRVMGIKDTERVGRLEEGITILRKLFDEENAS
                     HKGRFYEFEGVTIQPRPVQNPLPIWIASNPTGLTWKDGASAPSPAVERGLRRVAKFAD
                     GWMTNKVTPEEFKSQWTRILEMAKQEGRDPSKIGSSLYHNINIKENRQVALDESKAFL
                     DKYYTSNFSMNFVEGFTTAGTPKQCIQELKAYFGAGIEHITLRMTSWDQTGQMNRFLK
                     EVAPAFK"
     gene            13126..14700
                     /locus_tag="VF182_19405"
     CDS             13126..14700
                     /locus_tag="VF182_19405"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009112007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6439295.1"
                     /translation="MTATFRSYLEALKINGELLEITTATDARDIAALVPQAEQAILFK
                     NVTGYSMPVASGLLQSRNRLALAMGVPYEKIESKLRAAMDHPIAPQRVQHGPVKEVVR
                     SGNDLNLFDLPVPVFSIMDGGPMITGGVVIGEDPEFGMNAGVYRLMLKERDVTGIDIV
                     TPNNLRKFAERALQQKKPLPISISIGTHPYELIASTFKANLGVNELTFAGGLRGEPLM
                     LTEGETIPVPCIADAEIVLEGEILPEGWIHPEGPFCEFNRLMGGIHLNPRVRIKAILH
                     RKDPIYYALHMPWENIWMSAPIYEAAAWRVLHEAGVHATAINVTPGGCCHWHIVAGIK
                     RSPGDGKNAIMALLAIADIKHVTIVDDDIDVFDPTDVEWAVATRVQADRDVLIVSNAR
                     SKPLDPSLPPAATGKIPTTAKMGIDATIPENVPATRYNRIVYYNQGKVQLKDYLKSAG
                     AKPGTAAEPYDTQAVSEKILSELAESHRFFAELLERLPDVDYRAIAIALGTLHQQGKI
                     NQDGDGKYRMTQPTPP"
     gene            15603..16046
                     /locus_tag="VF182_19410"
     CDS             15603..16046
                     /locus_tag="VF182_19410"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6439296.1"
                     /translation="MPVRSGVLGHIVLNVADLKRSERFYRTVLGLKVSARNTRTKMTF
                     LSYGREHHDIALMQLPKGAKAVSGNGVPKLHHFCIYVESAREIDDLYPVLKRRKIPIV
                     SGPETLEVAGNRSISFLDPDGNRVEIACNANKYKYDNREKGLAQR"
     gene            16097..16876
                     /locus_tag="VF182_19415"
     CDS             16097..16876
                     /locus_tag="VF182_19415"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6439297.1"
                     /translation="MSGQMVLSGETLPTEDFCKVLIDEVKNRHARLHHPFYADLYDGK
                     LPLDAVRIWAKEAWGIFAYNVAINTAKLVRCQLSGIHDPEIHKKFVDIIHSEVGYKYF
                     EGSPRAVPGHRALFLRFGESIGIPAQELERCEREEDFLPTTVLARVGWLEIALRSNHI
                     LEQVAATNCCNEFSNQLTGGRFFKAFNRHYGLSAKDIEFFAEHGEADTEHSNIGYELV
                     EQFATTNDLQVRVLKALRKGLSIWWALTDGVARECARRDLH"
     gene            16904..17461
                     /gene="nbaC"
                     /locus_tag="VF182_19420"
     CDS             16904..17461
                     /gene="nbaC"
                     /locus_tag="VF182_19420"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HEX6439298.1"
                     /translation="METLSTFNLKRWVDENRHLFNPPFRTNQLIVHHKDFLVMILRGP
                     NTRLDFHIEPGDEFFYQIEGDMELHLKPPGERREIVTIKQGEIFVCPGDLPHSPRRYE
                     NTWGLVIERKRRDEEKEEFAWFCEKCDELILSRVVNQGNIPSQVSTVYREFNENVQIR
                     TCQACGYVFPPTPMAERLSFLESKK"
     gene            17694..18377
                     /locus_tag="VF182_19425"
     CDS             17694..18377
                     /locus_tag="VF182_19425"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6439299.1"
                     /translation="MILGHLPLRQAVIDAANRLVRVGAISMSHHGNFSVRVPNTDTIL
                     LTATSSFDNLSPENLALLDLGGRLLEGEINPTNAEIIHMHAVVYKQRPETGAVVHTHS
                     TYATSFALASRSLSCSYEALVRAEMTDGVPLAHYGPRGSAASVDNIAAALSSAAHTKA
                     VLLENHGVLAFGADPAAAARANLIVEEAAQMAIFAEMLGGAKEIPADLLRATVTRRDE
                     FARAGTKRL"
     gene            18433..18720
                     /locus_tag="VF182_19430"
     CDS             18433..18720
                     /locus_tag="VF182_19430"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX6439300.1"
                     /translation="MAIRLIVTIAAAPGKGAELARLYSAYCADVMKEPGCEQFEVFQS
                     AVNPDKLVLLERWTDKAALDVHALTSVRPTLPPELRVGTSEREDYEFNRTR"
     gene            19055..20008
                     /locus_tag="VF182_19435"
     CDS             19055..20008
                     /locus_tag="VF182_19435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439301.1"
                     /translation="MKEILSLILAVLLLSTGFGLIHYPAASGLTLAAESDEALQPLIT
                     TSELIVGENRFAFGLIKRDTLLEGANVQLRIYSIDGPQAQLVAETNALYYPVGAAQAG
                     EQVHRHADGTKHVHRADSDVRGLYVSRLTFAHPGPWGVELQVRDDSGALATARLTVTV
                     SNAPQTPALGSPAPRSRNVVATDVRDLRMIDTSQKPDPRLHQIRIADAIAQRKPQLIV
                     FATPQFCTSRMCGPVLDIVRQLIPEYNKRVAFTHQEIWQDFAGKKVFPTVEEWRLVSE
                     PWIFIVDGDGIIRAKFEGLVMGKELEAALQEVLQLNPPRHH"
     gene            20200..>20603
                     /locus_tag="VF182_19440"
     CDS             20200..>20603
                     /locus_tag="VF182_19440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439302.1"
                     /translation="MKRSLILIMALLSFWVIAPNAMSQTVDESRLSGRRADTTVSFGL
                     WQTDPPLNRFAGNPAMGAGNHHEVIPNVSRIDISRKDNGGVNFIISGNHVVAIYDDGT
                     RPEDIDPNLLVPNITTAGGVIDDPDKRIYRGV"
BASE COUNT         4805 a         5506 c         5619 g         4673 t
ORIGIN      
        1 gctttcaaag ggggacacta gctcttgggc atagtgatca atttgaccgg catcgacatc
       61 aaccgcgcct ttgacggtct tggcatattc cgcggcgtac agccccgcgc gccggccgaa
      121 caccaacaga tccgaaagcg agttgccgcc tagacgattc gcgccatgca agcccgcggc
      181 gacttcaccg gccgcgaaca gcccaggcac agttgtcgcg gtgctgtctg cgtctactct
      241 cactccgccc atgctgtagt ggcaagtcgg ccccacttcc atgggctctt tggtgatatc
      301 aacgtctgcc agttccttga actgatgata catagacggc agccgtcgtt ggatgtaatc
      361 cgttggtcgc cgtgaggcaa tgtctaaaaa aactccgcca tggggtgtgc cgcgaccggc
      421 cttgacctcg gagttgatgg cgcgcgcgac ttcgtcacgc ggtaacaagt ccggggttcg
      481 ccggttgttc ttcttgtcct cataccaagc gtccgcttcc ttttccgttt ccgcggtctc
      541 ggcgcgaaaa aactcaggga tgtagttgaa catgaatcgc tcattcttgg tgttcttaag
      601 cgtgccgcca tcaccacgca ccccttctgt cactaggatg ccgcggacgc tcggcggcca
      661 aaccatcccc gtaggatgaa actgcataaa ttccatgtcc attaaatcag cgcccacatc
      721 caaggccatg gttacgccgt cgccggtgta ctcccaggag ttcgatgtca ccttccaggc
      781 tttgccgccg ccgccggttg cgagcacgac cgccttgcat ttaaagacga tgaacttgcc
      841 ggtttccctc cagtacccga aggcgccgct gatttttccg ccatttgtca gcagccgctg
      901 gaggttgcac tccatgtaga cgtcaatccc tttgtggacc gcgtgctgct ggagcgttcg
      961 gatcatttcg aggccggtgc gatcgccgac atgggccagc cgagcataac ggtgcccgcc
     1021 aaaatcgcgc tgcaaaatca aaccatcatg ggtgcggtcg aacaacgcac cccattcttc
     1081 gagttcgagt acacggtccg gcgcttcttg cgcatggatc tgtgccattc gccagttgtt
     1141 gagaagtttg ccgccgcgca ttgtatcgcg aaaatgaacc ttccaattgt cttccggata
     1201 aacgttgccc atggccgcgg caatgcctcc ttcggccata acggtgtgcg ccttgcccaa
     1261 cagtgatttg cagaccaagg cggttctggc tccttgtgcc gaagcctcca cggcggcgcg
     1321 aaggcccgcg cctcccgcgc cgataatgac tacatcgtag tcgtgggttt cgtatttttc
     1381 ttcagccatt aaaagaacct caaatcctga atcacgccca ttgacagcag tcggacgtaa
     1441 aggtcggtca aagcaaccca gatcaagctt atccaggcat actccatatg gcgcccgtta
     1501 aggcggctta taaaactcca aacgttatat cgctttggcg cactatggaa tgagtccaga
     1561 taacccccac agacatgccg gcatgagttg caagaaagac tgaacagagt gagaagaata
     1621 gcgttgatga gaatgacgag ggtacccaaa ccgatgccga aaccattcgg aaaccggaat
     1681 gccaaaaacg cgtcccacca aagaaaagcc aaaactaaaa ttgataagta gaagaaatag
     1741 cggtgaaggt tttgtatcag gaacgggaag cgggtttcac cgctgtatga tttcgaagcg
     1801 tcccgcacgg cacaggccgg aggcgatagg aagaaagacc tataataagc tttgcgatag
     1861 tagtagcagg tggcgcgaaa tccgcccgga atccatagaa tcaaaaatgc cggtgacaaa
     1921 ttccaccacg atcccaccaa cggaactgaa acgtgagcgc agttctccgc gatgcacggc
     1981 gagtagaatg gcgacagata aggcgccaca taataatgcg cattctgtaa agccgcccaa
     2041 gtagcgtaaa tcgcgaaagc gccgagcacc agaaccacgg tcaacggctc tttccaccac
     2101 gtatctttcc gtaacgtgcg gaattggtcg agggttagag ttttattgct cacgtttgtg
     2161 tcctttgatt gttttcatca ttttagttga cggaccggtt gccgttaaaa tcgcgcaagc
     2221 cgaacatttc atacgaggca ttcaattaca ccaagccgcg gttcgctcaa ctcgaaacag
     2281 gaaaccgtgg ctctcgcttc cgtgaaatct aaggcaggcg attgttgatg cgcaatggca
     2341 gttaattggc cgcgacagtt tcctctttga ttttttcgct ttctagttaa aggttaagtc
     2401 ttgcagatct gcagaattcc tcgttggtta cttttcggtc ccagagtccc ctgacgcagc
     2461 cgctaccgga ccggagtctg cgtttcgatt gacgatttct gaagatcgtt ccgcattgtc
     2521 gtcaccgccc ccgggacggg ctctcgtcgt tacggtcgtg gatccaaaca caaattttgt
     2581 ctgaaagttt gttcgtggat aattaccctc ggcttataac agaaacaaaa ttgctttgca
     2641 cgcgctgagt tagtgctttt ctcgtccggg catggtcatg ttccaaggct cagaactttt
     2701 ttcgagttgc tccgaagtaa gcgcagtctt gagccgcgcc acttcccgaa cggacttgca
     2761 ggttgcaacc gcttcatgcg cgccggccgc ggcggcatca taaccgatcc atcgagccag
     2821 cgcggttacc atgacactgg ttttttcaac caactccctg caacgagatt tgttcccctc
     2881 gatacgatga cgcattcttc agtcaatgca ttgatcgcat tagggcaaac tcaaccgcct
     2941 gagcagtcta agcgtaacaa ctggcatcgt cacattcaat tcgaagtttc ccatctggcc
     3001 gcaaagcgta atcgtcgcgt ctactgaaat gatgcaggtt gtgagatagt actctgatca
     3061 actagtggag agcgaagcgt ggtcattgat gagcttgcta aagcgctgga tgcatcgaat
     3121 acacggattg tgcaatgccg aagatgtccg cggctggttc gctggcgtga aacttgtgct
     3181 aagactccac cgcggcgcta tcatggagtt gaatattggg ccagaccgct cgctggattc
     3241 ggtgatcctc acgcccggct tttaatcgtc gggcttgccc cggcggccaa cggcggcaac
     3301 cggactggac gcatgtttac cggagacaga agcggtgact ggctttacgg cgcgctccat
     3361 gcattcggct ttgccaacca gccgacatcg gaacatcgcg atgacggact ggagttaacc
     3421 aactgctata tcaccgccgc agttcattgc gcgccgccgc tgaataagcc ggcgttgatc
     3481 gagtttgaga ggtgccgacc gtttcttgtt cacgaattga aattaatgac gcaggtgcgc
     3541 gtggtcgtcg ttcttggaaa aatcgctttc gattcttttc tcaaggcgta taaggaagcc
     3601 ggcaggaaaa ttccgaatcc gcggccgaaa tttggacacg gaatcgcgac aattctgccg
     3661 gatggcgtta ctctaatttg ttcctatcat cccagccagc aaaatacttt taccggtaag
     3721 ttaacccagc cgatgtttta taaggtcttc gatcatgcta aggcgctgct gtgattttcg
     3781 atcggaattg cccagaaacc tcggcgaaat tcgtgattgc agcggtagct gcttgtacgt
     3841 atcctcaatt acaagtcaga aacaatcggc aaagttatcg tgatcgaact atgcgcggcg
     3901 gtcccctgcc gctctgattt cggccggtag gatagtgttc atgaatggtt ttaggaatcc
     3961 atgagctatc aaagaccggc gaagggacgt cgatgggtgc acccatgaaa aaccaattta
     4021 aggatgttgc ggccgaattg cgtaagcggc ttgagggtga ggtcaagttc gacccttata
     4081 cgcttctgtt gtactcaacc gatgcatcca tttatcagat tgagcccatc ggtgttgtcg
     4141 tgccgcgcca taagggcgac gtgcaggccg ctatcgaagt ggcaaatcgg ttcgatatac
     4201 caattctctc taggggtggc ggcacttcgc ttgccgggca ggcagtgggc catgcgatcg
     4261 tgctggactt ctccaagtac atgcgcaacg tcctggaggt aaacgaggaa gagctttggt
     4321 gccgggtgca acccggattg gtccaggatg agctcaatgc gcatgtgcgg tcactgggcc
     4381 ttcaatttgg accggatacg tcgacctcca atcgcgccac gatcggtgga atgattggca
     4441 acaattcggc cggcgcccac tcgctaacgt acggtaagac tctcgatcat gttttagagc
     4501 tgacggtgct tctatcggac ggtactgaag cagtgttaaa agatctctct tcgggcgggc
     4561 ttgcggccaa gatgcaagcc cgcggtctag aggggcaggt ataccgggag gtgtcgcgtt
     4621 tagccaaaga acatgagaac gaaatcgttt ctcgttatcc gaagatcatg cgccgggtat
     4681 ccgggtacaa tctcgacgaa ttcataaagc cgcagccgtt taaccttgcg cgtgtcatca
     4741 ccggctccga gggaacactt gccacgataa tcgaagcgaa aatgcgattg gtgcccaagc
     4801 ccaaatggac ggcgatggat gttattcatt ttcataacga tcaggaggcg ctggaatgtt
     4861 ctcagctggt cttgcagaca gcgccctatg cgatggaatc caccgacaaa atgatcctca
     4921 atctcgcccg tggcaatatc gaacaatcgc gcaaactggg atttattcaa ggacaacctg
     4981 aatcgttact gatggtggag tacgctggtg aaacccagga cgaggttaaa gcgcaggtga
     5041 gcaagcttga agacctccgc gcggcacagc gcatcggcta tgcggccacc caggcttaca
     5101 agccggaaga ggtcaaagcg atttggggag ttcgcaaagc cgggctcggt ttactgctcg
     5161 gcaccaaagg tgataaaaag cccatcgcat ttgtcgagga taccgccgtt gcgccggcca
     5221 agctgcccga gttcatcaag cgtttcaaag aaatcatcag cagccatgac gccatcgccg
     5281 gctactacgg ccattgctcg gtcggctgca tgcacatacg gcccctgatc aatctaaaag
     5341 agccagccga agtcgacaaa atggtttcca tttccaacgc gatttcagat ctggtcctgg
     5401 aattcgatgg ggcgatgtcc ggtgagcacg gtgatgggtt ggcgcgcagc cacttcaata
     5461 aaaagctctt cggcccggct ctctacgaat catttcgcca gatcaaacgc gccttcgatc
     5521 ctaagaatcg gctcaacccc ggaaaaattg tcgatgctcc ggcgatgacc gagtctctca
     5581 agatccatcc gaagtaccaa acttggatac cgcacaccac acttgatttc agcgcgcagg
     5641 gcggcttcgc aagagcagtc gaaatgtgca gcggtatggg cgagtgccga aaaaaactcg
     5701 aaggcaccat gtgtccttct tacatgggca ccttggatga ggagcattct acccgcgggc
     5761 gagccaatgc tttgcgaacc gttctctccg gcaaagtgcc taaggacgac ttcaccggca
     5821 agcggcttca tgagatcatg gatctctgtc tggagtgcaa agcgtgcaaa gccgaatgtc
     5881 cgtccaacgt cgacatggca aagctcaagt acgagtttct cgaccattac caccgcgcca
     5941 acggactgcc gctccgcaac cgtcttttcg gcggtattgc gcgcttgaat cgaattggcg
     6001 cccagtttgc gccagtggcc aacggaattg ccggttcaag gctcaaccgg tggctgatgg
     6061 agaagcttgc cggcatcgac cgccgcagac cgctgccaaa attagccggg cgaacttttg
     6121 agtcttggtt cggtaatcac agtggggaag gagacggctc taagggggag gccgtgctgt
     6181 ttcacgatac cttcaatgat ttcaacacgc ctcatgtcgc cattgcggcc acccgcctgc
     6241 ttgaacactt aggcttccgt gtgcttctga ccaacaaacg atgctgcggc cggcccatga
     6301 tctcaaaagg aatgttggcc gaagccaaaa acaatgccgt atggaacgtc gatcagcttg
     6361 cgccgtttgc cgagcgcggt attccaatca tcggtctcga accttcgtgc ttgttgacct
     6421 tgcgcgacga atatccagaa tttttgcgaa ccagcgctgc aaagaaagtg gcggcgcaaa
     6481 gctttctttt tgaggagttt ctggccagcg agctagcggc gggcagactc tccgttaaat
     6541 tgtctgggag ccgcggcaaa gcgctgcttc acggccattg ccaccagaaa gctttggtcg
     6601 gaacggcgcc cagcatcgcc atattgaggg cggccggttt cgaggtgagt gaagtcgatt
     6661 ccggctgctg cggcatggca ggttcctttg gctttgaaaa agagcactat gatctttcgg
     6721 tcaccattgg caatcggcga cttgcgccgg cggtcaaatc cgctggaacc gatgtcgaga
     6781 tcgttgctcc cggtatctcc tgccggcagc aaatcgaaca tctgaccgga cgcaaagcaa
     6841 agcatccggc ggaattgttg tgcgacactt tggcaccgag ctgcgaggat cgttgaagag
     6901 ccgctgattg gcacgaaaac ttctgaccat gacgctttga gtatctggta ggctagatcc
     6961 cccggcacgg acgtccgcac cgcctcaggc tctaaaagtt cgcatcttag aagcagaact
     7021 gttcgattct ggcagaacac aacccgccgg tgcacggcct ggcggcaggg ttgtgttcac
     7081 cggttggaag ctcaccgtgt ttccgccgat ttggacttct cggcagtttt ctccttagca
     7141 cgttctgctt tggttgtatc cgctttgggt gattcagttt tggcggattc gctcttcttg
     7201 gagtcagagc tcttggtcga tttgcctgaa tcggcaggct tgctcttggc gcctttttcg
     7261 gccgattgat cgatgactgt cttgccggta gtcgtcacct gcggccggat ctgtttcatc
     7321 aggccgggac ctatccccgg aactttttcc agatcatcca ctgatttgaa attaccgttt
     7381 ttagttcggt aattgatgat atcctgggcg cgtttctcgc caatcccctt tatgctggtc
     7441 agctcttctg tggtcgccgt attgatattc actgctgcca gggcaaggcc cgagagagca
     7501 aaccacatcg cgaatgccag taacagtttt cccatatcgt cctccttgtt ttttccatat
     7561 cgcacgactc tgttgattag acggtgttgt accattggcc ttcaatcgcg cagcggtttg
     7621 aggcggcaat gttcgatgcg acccgctgct gtcaatacaa gctcctccgg ctgcgcgcga
     7681 atatcagtca ccgtcaaatt tttctaggcc ggtgacccaa tcgcccgggc cgtgaaataa
     7741 cacccaaaaa ggcttgccag cttgtcgcca ataatcgttg acagattgga acacctctac
     7801 ggcgctgtga aatacttgag gtttacgtgc cgctagatgt ttcgagtttt caaagatgac
     7861 cacccagcct ctgccatcga gccaggagag atcggtcagg cagtcattga gagcatccca
     7921 attcttgccg aaccagtcag ggaagttgag cgcccgtgct acgcggccga taaggcctac
     7981 tttgctggca aggcctttca ggtcgattct aatcaccgta agcccggcac ttgcggccgc
     8041 ttgctcgatt tcgtccggat cgccgcttag ctggcagaca tcggattgtt cggcgttagc
     8101 gagctttctt gcgctcatgg tgataagcct ttgcgaactt cgcggaatgt ccggtaatga
     8161 tcctcggtat aatagcattc accgttttca ccggcgacaa tgcgtctggc accgcgatca
     8221 cgacgaccgg gtgtgggtac ggtaaattct cggtagtaac cgcgcttctt aagcggcaag
     8281 cgtcgctcga agttgccgaa caccgcgccc tcgcgcggat gtggaaaggg gcctcctttt
     8341 tcgatcaact gcagagtcgt tcgcgcttcg ctgggcaatt cctggagtgc gacctctttg
     8401 atccgtgtcc ttggttgatc ttgaccatag atagctaaga cccagctgtg gagtattacg
     8461 aagctgatga gtagggcaaa tagcctgatc acggcaggga cactgattgt ccggtatcct
     8521 aaagttccgg cgcttaaatc agagcagttg gtgcgccgcg gtattgattg gatcaccgcc
     8581 agtcagatgg cagtgattct gctatccgag tacatccgct acccgggcag ggtgaaagtc
     8641 taattaaacc gcgtcttcag gccgttcgta tttcgctttg cccatccgtt ctttgatggt
     8701 cggataagcg acggtgaaca ctgtcaaagc gatgagtccc atcaccgtcg ggaagagcag
     8761 ccactttgcg ccgtatgcgg cggtggaaat ccacaaatag ttctcagcga ttttacccag
     8821 ggtgagtcct aatatcagcg gtggccgagg ccagccatat ttcaccatga agtagccgac
     8881 gagtccgaaa atgagtgtaa aaatcacatc catgatatcg ttgttggcgg taaacgagcc
     8941 gataaaaacg agaaacatca ggaatgggat cagcagtgat ccacgaataa atgtgagcgc
     9001 tgccaggtga ttcaaaaaca gaaaacaaat tccggcgaca ataatattgg cgataatcaa
     9061 cacccaaacc atagaaaatg tcacatcgag ccgtgattta agcatctccg ggccaggatt
     9121 aagtccgacg atcagcatgg cgccgagcag tagcgccatg ctgccgctgc ccggaatacc
     9181 aaaagcaatc gtcgggatga gatcgccgcc gcgcgtggaa ttgtttgccg caccgggacc
     9241 gagcaggcct tcgatggcgc ctttgccggc cctcgactta tctttcaatc cggacacggc
     9301 atgggcatag gagatccatt gggcaacgtt cgctcctaaa accggtagaa cgccgaagta
     9361 gacgccgatg aggctgcaac gcacgaccag ccaaaaatgg cgaaacgtat ctttgactcc
     9421 gtccataacg ccggtaagct tgcctgtttt gccaccagcg atgctggtac cgcgcacggc
     9481 aagatcgacg atctctggta tggcgaacag accgacgaca acggggatga gatcgagccg
     9541 atcccacaaa tagagactgc cgaaggtata gcgcagcgag ccggtctgtg ggtcgacgcc
     9601 gatggccgcc aacatgagtc cgattccacc tgccagcaga cctttaatca gggacggacc
     9661 ggccaaggtg ccgataaagg tgatgcccat tatggctata atgaacagtt ccggcgagcc
     9721 gaacagcaaa acaagcggac gcatgatcgg aatcgaaagc gctagcgaaa ccgcgccaat
     9781 gatggagccc aagactgagc tcatgattgc ggcgcccagc gctcggccgg cttcgccgtt
     9841 cttcgacatt ggatagccgt ccaggatcag cgcggcggaa gaaccctcgc cagggatacc
     9901 gaataaaatc gaagtaagat cgcctgtcgt gccggtgacc gcgagcatcc ccaggagaaa
     9961 agcaaacgcg gaaatcgggt ctttgattga ataggaaaac ggcagcatca aagcgagtgt
    10021 cgataagccg ccgatgccgg gaagcaagcc gacccaaaag ccgatgaaga tacctatcag
    10081 catgtagccg aaggcgggcc acgctacgac ttgccaaaac ccactgacaa aagcttcaag
    10141 catgtccgat ctcctagacg ttgtttttcg tgcggatcat gcctatctca tccccgtgcg
    10201 gcggtcaaca acatccggag gttgcgttgc gcgctaacct atctgtcggg aaacgggaca
    10261 atgttatcgt gtcggaagat gggaccgcgc cttgcggaag gcacgattta ggcgtgacga
    10321 taatcgaggc atcgaggggt tggttctgtg aattccctat cgtccttccg attcatcgaa
    10381 tcgaaagatc gaactggccg gagtattaga gatcgacccg gagtttgtaa gcagatcaga
    10441 cattttcgag ttcgatctaa tagacacgtt gtacgagact gcgccagatc ggctatcctt
    10501 gtttgcgatg gagttgccgg cagaagtatt cccaaagatt gtgcaccgcg tcgggatgat
    10561 aaaagcgctt gcgcttgagc agggcttcgg cgtccgtcga ccaggagaaa gcgccgatgg
    10621 acgctgcggc aacctgcgtc tgagcgctgt gttcgaggag cacgccgcga acgcaggcct
    10681 cctcgatgga attcgcggcc acgacaatgc cgtggtttcg aagcagcacg attttgtgct
    10741 cgccgagtaa ccgagcaacg gccaccccct gttccggggt gacgatgagg tccgaagatt
    10801 cgtcaaattt tttcaacgcc ggcggaaaga aagcgcagct catgttggtg atcggtagga
    10861 gacggtgctc cgaagaggcg atgattgtcg agtagaccgg atgtgtgtgg atgacgcagc
    10921 ggatttcagg attggtccgg tacacttcgc tatgaatcgg aaattcattg tgccgcttgc
    10981 gcttgccggc tattttgttg ccggcgaaat cgataaggac cagatcgtcc tcggtaactt
    11041 cctccagccc tatgccagat cccttgatcc agtattgtcc gggagtgtca gtcaacgccg
    11101 agacatgtcc gtagatgaaa tcgttatgcc cgtgcatagc caaaatccgg caggccaaag
    11161 cgagtgtctc ttgcgttgtc atggcagtgc ccttctccag gtgaattagt ttgctccggt
    11221 tagctcgagt ctagcgccgt ggagcaggtg attatccaga gtctccggag cggcgccctg
    11281 gctgtgtaaa gtggcggagt agggcgatgc gccataagtg cgtatccagt caaccagcga
    11341 actcgatgcg ttgtagggca ttaaaaactc catcgaatga ggaccgattt gaaaagctaa
    11401 accttcagcg cccagctcgg ttcgcgcgat cggtttgccg ctctgctcca gaacctcgct
    11461 ataccaccgg tccacttccg acagctcgtt aacggctatt gtcaccctat cgataccgac
    11521 tgcgccattt ttgtgatgga actgttgggg gatacgttcg ttccgcggcg tgatgtcttg
    11581 aatcaggaac ggcgcaaacc cccgatgcga accggtcgcc agagaaagta tccattttaa
    11641 ttcgtagcca tcggggcgag tccgggacca cggcaccgga tcattgatcg caactccggc
    11701 ggcgcgcagc ttttgcgtat cgccttggag atcgtcggtt tgcaggcagt aatcgaccaa
    11761 gcgttctcca gtttgcagcg gttgccacca gcgatgatcg gacgcgtcac gatagaatcc
    11821 tatcaattcg agataagatc catcgctaaa cgagatgagc atgttatgac tgccgaccgg
    11881 atgttttccg ccgggaacaa ccgtaaagcc cagcttttca taatccgcgg ccgcctgtcg
    11941 cagatcttct acgacgatga ctaaatggtc gataccttta aacatggctc acacctgcag
    12001 gatgttccta cttaaacgcc ggcgccactt cctttagaaa acggttcatt tgtccggtct
    12061 gatcccatga cgtcattctc aaggtaatat gctcgatgcc tgcgccgaaa taggctttga
    12121 gctcttggat gcactgcttt ggcgtgcctg cggtggtgaa gccttcgacg aagttcatgc
    12181 tgaaattcga cgtgtagtac ttgtcgagga acgctttgct ttcatccaat gcgacttggc
    12241 ggttttcctt gatgttgatg ttgtgataga gcgagctgcc aatcttcgat ggatcgcggc
    12301 cttcttgctt agccatttcg agtatccggg tccattggga cttaaattct tccggcgtga
    12361 ctttgttggt catccaaccg tcggcgaatt ttgccacacg gcgcaaaccg cgttcgactg
    12421 cgggtgacgg cgcgctggcg ccgtctttcc aggtcaggcc ggtgggattg ctggcaatcc
    12481 agatcggcag cggattttgt accggacgcg gttgtatcgt tacgccttca aactcgtaaa
    12541 atcgcccttt gtgcgaagcg ttttcttcgt cgaagagttt gcgcaggatg gtgatgcctt
    12601 cttccaaccg gccgacgcgc tcagtgtcct tgatccccat cacccggtgc tcaagagcct
    12661 gcgccgggct ctgctcatta gggccaccca agcacaccgc cagccaagct cgaccggccg
    12721 aaatgacatc caggctggtc cattgctgcg caagcaaagc gggatgacgt tgcgggaacg
    12781 ttgccatgca gccgaccgcc aaacgcattt tttttgtgac aacggcgaga gctgacaaga
    12841 gcgtaatcga ttccaatcgc ggcttggcca ataagctgtc gccgacccag acggcatcga
    12901 atgcgcccga gtcctcgccg cgttttgcca tctcgaaaag ttccgggaca gtaacggcgc
    12961 cgatgatgac gccgcggttt gccagggtta acccaaatga ggccataatc cggttctcct
    13021 tactgctttc catagcatat gctccaccgg tttcgaaacc aatccggtga ttgacagcaa
    13081 ataatcgcta tggttgggtt acctgcaaat ccgcgagagg taacaatgac ggccacattc
    13141 agaagctatc ttgaagcatt gaagatcaac ggcgagttgc tcgagattac cacggccacc
    13201 gatgcgcgcg acatcgctgc gctggtgccg caggccgaac aggcaatttt gtttaaaaac
    13261 gtaactggtt actcgatgcc agtagcgtcg ggacttttgc aatcgcgcaa ccgactggca
    13321 ctggccatgg gagttccgta cgaaaagatc gaaagcaagc tgcgcgcggc catggatcat
    13381 ccgatcgcgc cgcaacgggt ccagcatgga cctgtaaagg aagtggttcg cagcggtaat
    13441 gatctcaacc tctttgacct gccggtgccg gttttttcca tcatggatgg cggccccatg
    13501 atcaccggag gtgttgtgat cggtgaggat ccggaatttg gcatgaacgc tggcgtctac
    13561 aggttaatgc tcaaggaacg agatgtcacc ggcatcgata tagtaacgcc gaataatttg
    13621 cgcaagttcg ccgagcgggc gctgcagcaa aaaaaaccgc tgccgatatc gatctcgatc
    13681 ggtacgcatc cttacgagct gatcgcatcc acttttaaag ccaatctcgg tgtcaatgag
    13741 ctcacctttg ccggcgggct tcggggcgaa ccattgatgc tcaccgaggg cgaaaccatc
    13801 ccggtgccgt gtatagccga tgccgagatc gttttggaag gagaaattct tccggaaggc
    13861 tggattcatc ctgaaggacc attttgtgag ttcaatcggc taatgggcgg cattcatctc
    13921 aatccgcgtg tacgcatcaa agcgatcctg catcgtaagg atccgattta ctatgcgttg
    13981 catatgccct gggaaaacat ttggatgagc gcgccgatat acgaagctgc cgcttggcgt
    14041 gtgcttcacg aagccggcgt tcacgccacc gccatcaatg taacgcccgg gggatgctgt
    14101 cactggcata tcgtggctgg gatcaagaga tcgcctggcg atggcaagaa tgcgatcatg
    14161 gcgcttttag ccatcgccga catcaaacat gtgactattg tcgatgatga tatcgatgta
    14221 ttcgatccga cggacgttga atgggccgtg gccacccgtg tacaggccga tcgtgacgtg
    14281 ctgatcgttt ccaatgctcg ttccaaacct ctggatccat cgctgccgcc tgccgctacg
    14341 ggcaaaattc cgacgacggc taaaatgggc atcgacgcaa ctattccgga aaacgtgccg
    14401 gccactcgtt acaatcggat tgtttattac aaccaaggca aggtgcagct aaaagactat
    14461 ctcaagagcg ctggcgcgaa acccgggact gcagccgaac cgtatgatac acaagccgtc
    14521 tccgaaaaga ttctcagcga gttggcagag tcgcaccgct tttttgccga gttgttagag
    14581 cggcttcctg atgtcgacta tcgcgctatc gcgatagctc tgggcactct acaccagcag
    14641 gggaagatca atcaggacgg cgatggaaaa tatcggatga cgcaaccaac gcctccatga
    14701 cggtcctcct aaattgccct gaggccgaac acaccgatac cgattctcta ttcaatctca
    14761 tttctgaacc agcgcctctt atcccgtccc gggcttaaga cacatcaata gatatacttg
    14821 cgaattttgt tagctggctc ttgtggcata accattgcgc agaagagggg aatctttcga
    14881 aggaggtttc aatatatgaa agggaaacgg ttattagcga tttcagcttc tgttctcacg
    14941 gcagcttgga ttacagcgga tgctggagtg accctcgcgc aaactaaaag caaggacagc
    15001 aaggtgaatc ccgacctcaa tccatctgtc ggtgcttcgc agtccgagag gacgggtagc
    15061 gacagtcaaa ctccgctccc ggagaagagc ccgatgacag ggacggtgga gaaaggccct
    15121 ggctcaaccg agggctctag ctctaccggc accaccaagg gctcccgctc tagtgggact
    15181 agcaagggaa cgagccagtc gcggacaaag gatgataaaa caataaatcc tgaccttaat
    15241 ccctccgtcg gtggttcgca gtcggaaagg acaggcagta gcggaacgcc gatgccgaag
    15301 aacagcccgg ccaccggaac cgtagagaaa ggcacgggct cttcgcggta gttgttctac
    15361 aatgtttcgg tgccggacag aagtcggcac tgagaaacac ttgccccaca tgcgggagtc
    15421 aatgttctct tttgactccc gcatcgtttt gtcgctggcc atcgccacaa catcttctcg
    15481 cgatatcttc aaagtcactc tgagcaacgt gtgattgcca cgatcgtgtg aagctttcat
    15541 cggcgcggaa cgatcagccg ttcgccttga cagacgtgag cgggcttaat atgagggttg
    15601 ctatgccggt tcgtagtggc gtgctcggcc atattgtctt gaatgtcgct gacctgaaaa
    15661 ggtcggaacg attctatcgc actgtgcttg ggctcaaagt ttccgcgcgc aataccagga
    15721 cgaagatgac cttcttgagc tacggtcgag agcatcatga tattgcgctc atgcaattac
    15781 ccaagggggc caaagcagtg agtggcaacg gcgttcccaa gctccatcac ttctgcatct
    15841 atgtcgagtc cgccagggaa atcgatgacc tgtacccggt cctcaaaaga cgaaagattc
    15901 caattgtcag cggccccgag acattagaag tcgccggtaa ccgttccatc agttttctgg
    15961 atccggacgg caatcgcgta gagatcgcct gcaatgcaaa taaatacaaa tacgacaatc
    16021 gggagaaggg cctggcgcag agatgaaggc gcaaggtgaa gaagcttatg aattccaggt
    16081 atgaagcgag gtgacgatgt caggccaaat ggtgctcagc ggcgaaactt taccgactga
    16141 agatttctgt aaggtgctga tcgatgaggt caagaatcgg catgcccgtc tgcaccaccc
    16201 gttctacgcg gatctttatg atggaaaatt gccgttagat gcggtcagga tctgggcgaa
    16261 agaggcttgg ggaatttttg cttacaacgt agcgatcaat accgcgaagc tagttcgttg
    16321 ccagttgtcg gggattcacg atccggaaat tcacaaaaaa ttcgtcgata tcattcactc
    16381 agaggtcggc tataagtact tcgaaggtag cccgcgggcg gtgcccgggc atcgggcgtt
    16441 gtttctccgt ttcggcgaga gcatcgggat tccggcccag gaactggaac gttgcgagcg
    16501 tgaagaagac tttctcccga cgacggtgtt agctcgggtg ggctggctcg agatcgcgct
    16561 gcgcagcaat catattctcg agcaagtggc agctaccaac tgctgcaatg agttctccaa
    16621 tcaattgacc ggcggacgat tctttaaagc gttcaaccgc cactatggat tgagtgcgaa
    16681 ggacattgaa ttttttgccg aacatggcga agcggacacc gagcacagca atataggtta
    16741 cgaactcgtc gagcagtttg ctacaaccaa tgaccttcag gtcagagtgc tcaaggctct
    16801 gagaaagggg ttatccattt ggtgggcgct gaccgacgga gtcgcgcggg aatgcgcaag
    16861 aagagatctg cattgatcgt agtgtttgct tcgatgttgc gcgatggaaa cgctttcaac
    16921 atttaatcta aagcgctggg tcgatgagaa ccggcacctg ttcaatcctc cgttccgaac
    16981 caatcaactt atcgttcatc acaaagactt cttggtgatg attctgcggg gaccgaatac
    17041 tcggctcgat tttcatatcg aaccgggtga cgagttcttt tatcaaatcg agggcgatat
    17101 ggagctgcat ctgaagccgc ccggcgaaag aagagagatc gtgacgatca aacagggaga
    17161 aattttcgtt tgccccggcg atctgccgca ttcgccccgc cgctatgaaa acacctgggg
    17221 gctggttatc gagcgcaaac gccgcgatga agaaaaggaa gagtttgcgt ggttctgcga
    17281 gaaatgtgac gaactgatac tcagtcgagt cgtcaatcag ggcaatattc cgagccaggt
    17341 ttcgactgtc tatcgggagt tcaacgagaa cgtgcagatc cggacctgcc aggcctgcgg
    17401 atacgttttt ccaccgacac cgatggcgga gcgattgagt tttctggaat caaagaagtg
    17461 agggctgata ttgcgccttg agacgtgtca cattggtatg cacgcggtca cttgcatccg
    17521 gcacattacg catccggttt cctgattgat cgcatgagag tgacataggc aacaattgca
    17581 caagatgttc tcgtgacgat ccgtgcactg gtcatcgagt taggctgctt cggcagttcg
    17641 ttcgcaaaac tgattcgttg ttgatgcgtc ccgacctcag cagaggagga gttatgattc
    17701 tcggtcatct accattgcgc caagccgtca tcgatgccgc gaataggctg gtgcgcgtcg
    17761 gcgcaatttc catgagtcat catggaaact tcagcgtgcg tgtgccgaat accgatacga
    17821 ttctccttac cgccaccagc tcattcgata atctcagtcc cgaaaatctg gcgctactcg
    17881 atcttggcgg acgactcctc gaaggtgaga tcaatccgac caacgccgaa atcatccaca
    17941 tgcacgcggt ggtttacaag caacgtcctg aaacgggcgc cgtggtgcac actcattcca
    18001 cttacgcgac cagtttcgct ctcgccagcc gctcgctatc ctgctcgtat gaggcattgg
    18061 tgcgcgcaga gatgaccgac ggcgtgcccc tggctcatta tggcccgcga gggtcggcgg
    18121 catccgtgga taacatcgcc gccgctttga gcagcgcggc tcacaccaag gcagtcctgt
    18181 tagaaaatca tggggtgctc gcctttggcg cagacccagc cgcagccgct cgcgccaact
    18241 tgatcgtcga ggaagctgcc caaatggcga tcttcgcgga aatgctgggt ggcgcgaaag
    18301 aaatcccagc tgatctttta agagcgacgg tgacacggcg agacgagttc gcgcgcgcgg
    18361 gcaccaagcg gctttaacac gggacggcgt ccaaatcgct gcaacgtcga ttaagctggc
    18421 gagggagacg aaatggcaat tcgattgatc gtaactatcg cagcagcacc gggaaaaggc
    18481 gcagagcttg cacggcttta tagcgcttac tgcgcagacg tgatgaaaga gccgggctgt
    18541 gaacagttcg aggtgtttca aagtgctgtg aatcccgaca agttggtgct gcttgaaaga
    18601 tggaccgaca aggccgcgct ggatgtgcac gcattgacca gtgttcgtcc gactctgcca
    18661 cccgagctga gagtaggcac gagtgaacgc gaagactatg agtttaatcg gacacgttag
    18721 gcatatgatg attttaattt gcaatattga atgaactgaa attggctcgt aacactctat
    18781 tggctagaag cagtcccaca tgtctctccg attagttctt gcagcgacgg cccacgaaaa
    18841 gatggcaaag atgttgtcac ttcaataaca agctcagcgc accgccgagt gtcgaactct
    18901 gaccagttat tttacgacaa acaacgctgc aacagacctc atcaaaattt tattgacatg
    18961 cgccgcgcag cagagtagat gatcaattac tcctcgatca gcgctcacag agataatttc
    19021 atcataactt cttggactct caggatggat agttatgaaa gagattttgt ctcttatcct
    19081 cgcagtgctc ctcctatcga ccggtttcgg tcttattcat tacccggcgg cctcaggtct
    19141 gacgttagca gcggaatcgg atgaggctct tcaaccgcta atcaccacat cggaactgat
    19201 tgtcggagag aatcgcttcg cctttggact gatcaaaaga gacacgcttc tggaaggcgc
    19261 taatgttcag cttcggatct attccattga tgggccgcaa gcacaactag tagcggaaac
    19321 caacgctctt tattatccgg tcggggctgc gcaagccggt gagcaggtgc accgccatgc
    19381 tgacggtaca aagcacgtgc atcgcgccga cagcgatgtg cgcggtcttt acgtcagtcg
    19441 tctcacgttc gcgcatcctg gtccctgggg tgtcgagctg caggtgcggg atgatagtgg
    19501 cgctttggca accgcacgtc tcacagtgac ggtgtccaac gcgccgcaaa cgccggcttt
    19561 gggttctccc gcgccgcgca gccgcaatgt ggttgccacc gatgtgcgcg atctgcgcat
    19621 gatcgacacc agtcagaaac cggatcctcg actacatcaa atacgaatcg ccgacgcgat
    19681 cgctcaacga aaaccccagc tcatcgtctt cgccacgccg cagttctgca cgagccggat
    19741 gtgcggccca gtcctcgata tcgttcggca gttgatccct gagtacaaca agcgagtcgc
    19801 ctttactcac caagaaatct ggcaggattt cgccggtaag aaggtctttc caactgtaga
    19861 agagtggcgc ctggtatccg agccgtggat ttttattgtg gatggcgacg gaatcatccg
    19921 ggccaagttt gaagggcttg taatgggcaa agagctggaa gcggcgctac aggaagtgct
    19981 ccagttgaat ccaccgcggc atcactaggc tcggtatcag ttattcaaag gatctggaat
    20041 gcacaacacg aatattgaaa cacacgcggc tagatatcct cggttgattg aaagctgcct
    20101 cccgaaggtt tttccgtggg ccttcgcgga ggcgaagacg tcgcgccacg gagttggtgt
    20161 cggaaatcac gtattgaacc gcatgaggag gagaaaggaa tgaaacgttc cctgatatta
    20221 atcatggcac ttttaagttt ctgggttata gccccgaacg ctatgtctca aaccgtggac
    20281 gagtctcgac tgagtggtcg ccgagctgac acgacggtaa gctttggcct ttggcaaacc
    20341 gacccgccat tgaatagatt tgctggcaac cccgccatgg gcgcaggcaa tcaccacgaa
    20401 gtaataccta acgtatcgag gatcgatatc tcacgaaagg ataatggcgg ggtgaacttc
    20461 atcatcagcg gcaatcatgt cgtcgccatc tacgatgacg gcacccggcc cgaggatatc
    20521 gatccaaatc tgctggtccc taatataacc accgcgggtg gcgtcatcga tgatccggat
    20581 aaaagaattt accgcggtgt caa
//
LOCUS       DASUVS010000363         5309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1023998, whole genome shotgun sequence.
ACCESSION   DASUVS010000363 DASUVS010000000
VERSION     DASUVS010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023998"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..1135
                     /gene="gloB"
                     /locus_tag="VF182_19445"
     CDS             368..1135
                     /gene="gloB"
                     /locus_tag="VF182_19445"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEX6439303.1"
                     /translation="MKIIQVPLLRDNYAYLLVCEKSKEAAVIDPSEAEPVIKRIEQEK
                     ITLTSILSTHHHRDHTGGNEGILAQHKVDVYGHKSDAARIFGLTRGIEEGDEIKVGDL
                     TGKVLFIPGHTSGHVAYLFGGNLFCGDTLFTAGCGRLFEGTPDQMHGSLKKLAALPDE
                     TKVYCGHEYTESNLRFAVTLEPKNHKLVSRFERVQGLRTRGASTVPATLEEEKQTNPF
                     LRWDSKELQMTLKSQNPDLRLDPVSVFAKVRQLKDSF"
     gene            1431..2543
                     /locus_tag="VF182_19450"
     CDS             1431..2543
                     /locus_tag="VF182_19450"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6439304.1"
                     /translation="MTITGAATPGGTERYRDRFGGRIHREHYRQSQSLWMSSIGIGTY
                     LGDYDAETDGEYCQAINRAVQCGCNVIDSAINYRLQRSERAIGAALQDLSAKGFQRDE
                     IIVASKAGFIPYDSAPPADVRRYFEETFFQPGIVSAADIISNSHCMTPAYLLNQLDCS
                     LKNLSLECLDIYYIHNPETQLGKISREEFNSRLFKAFEAMEGAVSAGKIRMYGTATWN
                     GYRNDPQAKDYLSLAETIGLATKAGGKDHHFKVIQLPFNFGMTEALSLSNQTVGGKTL
                     TILEAAQAFDITVMCSASILQGQLAANLPSMIGAALTGLETDSQRSIQFVRSTPGVTT
                     ALVGMKQLKHVEENLRTARVPPAPWEQYSKLFESNP"
     gene            complement(2744..3724)
                     /locus_tag="VF182_19455"
     CDS             complement(2744..3724)
                     /locus_tag="VF182_19455"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6439305.1"
                     /translation="MAGTIGTEGFDAMEYTTLGRTGLKVSVAGLGCGGPSRLGLRDNA
                     AAEAGAVSLVRQAIDLGINFLDTAQSYGTEPVVGKAIAGVARDKLIISTKKTLPPADH
                     SNPAEAIIQGLEQSLRSIGTDYIDIYHLHGVEPKDYVFAKEKLMPAMRRLRDQGKIRF
                     IGVTEGFVTDTSHQMLQQSLREDLWDVVMVGFNLLNPSARRTVFPLTAAKEVGVLDMF
                     AVRRTLSQSDRLRSLCGELIQKGVIPRSVVDEDNPLGFLLEKGVAATIPEAAYRFCRH
                     EPAVDVVLTGTGNPEHLKANIQAVLKPPLPQPALQRLEKIFGNLDSLTAN"
     gene            complement(3823..4278)
                     /locus_tag="VF182_19460"
     CDS             complement(3823..4278)
                     /locus_tag="VF182_19460"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX6439306.1"
                     /translation="MNGQAFQDQGTVRYCHGCGADNDQGLQIKSYWDGDEAVATWHAQ
                     PHHCGGTKQNVNGGIIASLIDCHSLNLAIAHAYKQEQRPIGSPPRIGYVTANLNVSYL
                     KPTPVAEPIQLRARIIKLDGRKAWVNCVLTAASELRATGEVLGVRVEWD"
     gene            complement(4292..4696)
                     /gene="msrB"
                     /locus_tag="VF182_19465"
     CDS             complement(4292..4696)
                     /gene="msrB"
                     /locus_tag="VF182_19465"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX6439307.1"
                     /translation="MSESKEKIEKTDEEWRKELSPEQYEVCRQKGTERAFTGKYWDCH
                     DQGTYHCACCGNELFSSDSKFDSGTGWPSFYQPIADDKVDTETDNSFLMRRTEALCSR
                     CNAHLGHVFDDGPAPTHLRYCINSASLKLEKK"
     gene            complement(4724..5215)
                     /locus_tag="VF182_19470"
     CDS             complement(4724..5215)
                     /locus_tag="VF182_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX6439308.1"
                     /translation="MASFDVVNRIDLQEVDNAVNITKKTLQSRYDFRESKTEVVLDKK
                     DKKINLTTEDEMKMRAVQETLVENLVKRKVDTRCLEYKPSEMAAQGMIQKTISIKEGV
                     DSDTARAIVKMIKDQKMKVQAAIQEKQVRVTAKKIDDLQAVIKLLRSSNLPIPLQFIN
                     MQS"
BASE COUNT         1211 a         1449 c         1377 g         1272 t
ORIGIN      
        1 tctgacctgc acgatatcga tctcgcggtc atgaaacccc gctgcggaac tctaatcttt
       61 ggctactggg tatatgccag ggatcagcaa aatatcaact gctacaagaa ggactgccgc
      121 tcatcccgaa attagtgcga aacgcctgcc cgcggcaacc aagatagagg aaccatttcc
      181 atcggaaatt ttattgccaa ctgctttggc ccctggttta cccgtacgag gctccgacga
      241 gctgcggcgc aacgaggcac gatgtttctg aattcaattc aagagccttt tgcttttgat
      301 aggcgatgat ttgtgatagc ctctgcgctg ggtttcgtcc caatccgaaa ggcgcattcc
      361 taaacacatg aaaatcatcc aggttccgct attgcgcgat aattacgctt atctactggt
      421 ctgcgagaag agcaaagagg cggcggtcat cgacccttct gaagccgagc cggtaataaa
      481 acggatcgaa caggaaaaga tcactctcac ctccattcta agcacgcatc accaccgcga
      541 tcataccggc ggcaacgaag gtattcttgc gcagcacaag gtcgatgttt acggccataa
      601 gagcgatgct gcgaggatct tcggcttgac acgaggaatc gaggaaggcg atgagatcaa
      661 ggtcggggat ttgaccggca aagttctttt tattccgggt cacaccagcg gccatgtcgc
      721 ttatctcttc ggcggcaatc tattttgcgg cgatacccta ttcaccgcgg ggtgcggccg
      781 gctcttcgag ggcacgcccg atcagatgca tggatcgcta aagaaactgg cggcgctacc
      841 ggacgaaaca aaagtttatt gcggtcacga gtatactgaa agcaatctcc gttttgccgt
      901 gacattggag ccgaagaacc acaagctggt atctagattt gagcgggtgc aaggattaag
      961 aacgcgaggc gcttccacgg ttcccgccac cctggaagaa gaaaagcaga ccaatccgtt
     1021 cttgcgctgg gatagcaaag agcttcaaat gaccctcaaa tcccagaatc ccgatctccg
     1081 tctagatccg gtttccgtat ttgcgaaagt acgccagcta aaggattcct tttaacgggc
     1141 ttgttctcag caaggctgac gcaaagttct acagcggtca cgtatcgcgc ctcctgagcc
     1201 atcatgacga tctgcccggt gagccttcac tcaggcgcag atggatcccg caactcctgc
     1261 gcgaaggaac ttttcacaac gaagcagtct ctgttacact gttcttcctc gctcggcggc
     1321 gtactcccat ctggttagaa aatgttcagc gccgcgcggc atgatttcta caatgcgcag
     1381 gagcttgggt tggacgcttc attcttgagc gacaaaggca aaggtacacg atgacaatca
     1441 ccggcgcagc gacacctggg ggcacggagc gttaccgcga ccgttttggc ggcagaattc
     1501 atcgggagca ttaccggcaa tctcaaagtc tttggatgtc ttccatcggc atcggcactt
     1561 atctcggcga ttatgacgcc gagaccgacg gtgagtactg ccaagctatt aatcgcgcag
     1621 ttcagtgcgg ctgcaatgtg atcgacagtg cgataaatta ccgcctgcaa agaagcgagc
     1681 gcgcgatcgg cgcggcattg caggatcttt cggccaaggg atttcaacgg gatgaaatca
     1741 tcgttgcgag caaggcagga ttcatccctt atgacagcgc gccgccagcc gatgtccgga
     1801 ggtatttcga agaaaccttc tttcagcccg ggatcgttag cgccgccgat atcatcagca
     1861 actctcattg catgaccccg gcttatcttt tgaatcagct cgactgcagc ctgaaaaatc
     1921 tttctcttga atgcctcgat atctattaca tccacaatcc ggagacgcag ttaggcaaaa
     1981 tatctcgtga ggaattcaat agtcgtttat tcaaagcttt tgaggcgatg gaaggggcag
     2041 tgtcggcggg aaaaatccgc atgtacggca cggcgacatg gaacggctac cgcaatgatc
     2101 cgcaggccaa ggattacctg tctctggcgg agaccatcgg tctggcgaca aaagccggcg
     2161 gcaaagatca ccattttaag gtgatacaac tgcccttcaa ttttggcatg acggaagctc
     2221 tgtccctgtc aaaccaaaca gtcggaggca aaactcttac gattttggaa gcggcgcaag
     2281 ccttcgacat cacggtcatg tgcagcgctt ccattctaca gggtcagctc gccgccaatc
     2341 ttccgtcgat gatcggcgcc gcattaaccg ggctcgaaac cgacagccag agatcgattc
     2401 agtttgtgcg ctccaccccg ggggtcacca ccgctctggt cggtatgaaa cagctcaagc
     2461 acgtggaaga gaatctgcgc acggctcgag tcccacccgc gccatgggag caatattcaa
     2521 aactattcga gtcgaacccg tagactcagt aggcacccgt gatttcgcac gccactagct
     2581 agcttagcag attactaaaa aactctggtt gcaggctgct caaaaagatt caggggctag
     2641 gcgcggcgaa tcacaaggat gaaagcggaa atctgagagc ggaattcagt tttcagcttt
     2701 cataattcat ctttccgaag catacgggtc ttttcgcgcc ctgttagttt gcggtgagtg
     2761 aatctaagtt gccaaatatt ttttccaatc gttggagtgc cggttgcggc aagggcggct
     2821 tcagaacggc ttggatattg gctttgagat gttcgggatt accggtgccg gtgagaacga
     2881 cgtcgacggc gggttcatgc cggcagaaac gataagccgc ttccgggatt gtcgcggcga
     2941 ctcccttctc gagaagaaat ccgagcgggt tatcttcatc gacaacgctc cgcggaatca
     3001 ctcccttttg aatcaattcg ccgcataagc ttcttaaacg atccgactgg ctcagagtcc
     3061 tgcggacggc gaacatgtcc agaactccaa cctctttagc tgcggtcaat gggaaaacgg
     3121 ttctgcgcgc cgaaggattg agaagattga agccgaccat caccacgtcc catagatcct
     3181 ctctcaagct ttgttgcagc atctgatgcg aggtatcggt cacaaacccc tcggtcacac
     3241 cgatgaagcg aatcttgccc tgatcgcgaa ggcgccgcat ggccggcatc aatttctctt
     3301 ttgcaaagac ataatctttc ggctcgaccc catgaagatg atagatatcg atgtagtcgg
     3361 tgccgattga tctcagactc tgctccagtc cctggatgat cgcctcggcg gggttgctgt
     3421 gatcggccgg aggcaaggtc ttcttggtcg agataatcaa tttatcccgc gctactccgg
     3481 caatcgcttt cccgacaaca ggctcggttc catacgattg agccgtgtcg aggaaattga
     3541 tgcccaagtc gatggcctga cggaccagcg aaacggcgcc ggcttccgcg gcggcgttgt
     3601 cgcgcagccc gaggcggctc ggcccgccgc aacccagacc cgcaacactt accttcagac
     3661 ccgtgcgacc tagcgttgtg tattccatgg catcaaaacc ttcagttcca atggttccag
     3721 ccattccagt agttccaaat gttaagactg taactgcttg aacgatcgga acaccggaaa
     3781 tgcttctcga acgttagaac cggtggaacg tttggaactg tcctagtccc attctacccg
     3841 aactccgagc acctctccgg tcgctcgcag ctcactggcg gcggtcaaca cgcaattgac
     3901 ccaagccttg cggccgtcga gtttgataat acgcgcgcgc aactggattg gctctgcaac
     3961 gggcgtaggc tttagatacg acacattcaa attcgcggtg acgtagccga tgcgcggcgg
     4021 actgccgatc ggacgttgct cctgtttgta ggcatgcgca atagcgaggt tcaagctgtg
     4081 acagtcgatg agcgaagcaa tgatgccgcc attgacgttt tgctttgtgc cgccgcagtg
     4141 gtgcggctga gcatgccagg ttgctacggc ttcatcgccg tcccaatagc ttttgatctg
     4201 caaaccttga tcattgtccg cgccgcagcc atggcagtag cgaaccgtgc cctgatcctg
     4261 aaatgcttgc ccattcatcg aaaggaagga tctacttctt ttcgagtttc aacgaggccg
     4321 aattgatgca gtagcgcaag tgggtcggcg ccggtccgtc atcgaagacg tggcctagat
     4381 gagcgttgca tcgactgcag agcgcttccg tgcggcgcat gagaaagctg ttgtcggttt
     4441 ctgtgtcgac cttgtcatcg gcaatcggct ggtaaaaact cggccacccg gtgccggaat
     4501 cgaatttgct gtcggaactg aacagctcgt tgccgcaaca cgcgcagtga taagttccct
     4561 ggtcgtgaca gtcccagtat tttccggtga aagcgcgttc ggtgcctttc tggcggcaca
     4621 cctcgtactg ctccggcgac agttctttcc gccactcttc atcggttttt tcaatctttt
     4681 cctttgattc actcatggtt tcctcctgaa ccaccatgcc tccttagctt tgcatattga
     4741 taaactgcag gggaatcggc agattcgaag acctcaacag tttgatgacc gcctgcaaat
     4801 cgtcgatttt ttttgctgtg actcgaacct gtttttcctg gatggctgct tgaaccttca
     4861 tcttctgatc tttgatcatc ttaacaatcg cccgggccgt atcggaatcg accccttcct
     4921 tgatcgaaat ggtcttctga atcatcccct gggccgccat ttcgctgggc ttgtattcca
     4981 aacatcgagt gtcgactttc cgcttgacca gattttccac caatgtctct tgcaccgcgc
     5041 gcatcttcat ttcatcttcg gtcgtaaggt tgatcttctt gtcttttttg tccagcacga
     5101 cctcggtttt tgattcgcga aaatcgtagc gggattggag tgtcttcttg gtgatattca
     5161 ccgcgttatc gacttcctgc aggtcgatac ggttaacaac atcgaaagac gccatgaatt
     5221 tctcctgctc tagcagacct actgaaaagt cgccggtgct tcgtgcgcga tcgccgcctc
     5281 atcggaagtt gagtactgga gtggtggag
//
LOCUS       DASUVS010000364        17270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_372544, whole genome shotgun sequence.
ACCESSION   DASUVS010000364 DASUVS010000000
VERSION     DASUVS010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 17270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17270
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372544"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..761
                     /locus_tag="VF182_19475"
     CDS             315..761
                     /locus_tag="VF182_19475"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6439309.1"
                     /translation="MQKSIVVGLFILLTASVSSQVLAQEDFIEKRQKAMKGNSADVKA
                     IKGAVESKDYATIETKAKDIMGTADKIPSLFPKGSTTGKTKAKPVIWEKSDEFAKNAK
                     NLSKAAGELATAAKSQDESAVGTKFKALGEVCSSCHKEFRADKYSE"
     gene            complement(810..1667)
                     /locus_tag="VF182_19480"
     CDS             complement(810..1667)
                     /locus_tag="VF182_19480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6439310.1"
                     /translation="MIPGSFDYFAPRSITEAIEFLSAHRDDVKVLSGGQSLLPLMKMR
                     LSKPGYVVDIGKIPGLDMIDEEGNGLVLGALVTHAQIEYSELLQKRCPLLPQTAATIA
                     DIQIRNRGTIGGSVAHADPAGDMPAAIMALDAEIKVAGPSGERWIKADDFFLGLLMSV
                     LEPDEIVSAIRVPVTDADKTAYLKAAPRSSGFAVVGVAVRLTMDSTGTCTRAALGITG
                     ITDKAYRPSRSEEILTGKKLEPKIIAEASAESTRNIDVIEDINGSSEYRTHLTHVYVE
                     RAIEAALQC"
     gene            complement(1670..4096)
                     /locus_tag="VF182_19485"
     CDS             complement(1670..4096)
                     /locus_tag="VF182_19485"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6439311.1"
                     /translation="MPDFIGSRTRSKEGPRHVTGRGLYTDDFILPGMLQAMILRSPHA
                     HAKILSVDPSQALANPNVIAVITPDDIKQSTKPFKPGRYAAGLKRPIDEYAGAVDKAR
                     YVGEPLGAVAARDRGTAEDALELISVEYEPLRPVVDVREAIKPSAATLFDELGSNLAW
                     HGSLQYGDIDGAFKSADRVVKENLKIHRYSSTPLEPFVVIASYDPASKRLTVWVTAQV
                     PEVIYDGLREALGLQDVRVIIPDVGGGFGQKIHLIRKYVVMVALLSMKTGRPVKWVED
                     RSEHMMAGGHACAQEFEAEAAVKNDGTVLGLRFKEYDDVGGSISTLTIHFTNKLNNLS
                     NTYRTPSIAMEGFAVVTNKCPVIPNRGIGKPGMCYIWERMMDRVAQDLNMSPIEVRRK
                     NLIQPGEMPYRTISGNVYDSGDYPGLLSTLLEKIDYDKLREEQKREREKGRLIGIGIV
                     VGVEPGGRNAARDMAIFPEMKEPPGSGGVNGATIKLEKNGTIALHLGSPNCGQAHETT
                     TAQVAADVLGTSPDQISTSIPFDSDLSPWGVAAANSGNNFHLYDIGAIHGAAKKLREK
                     VVKLAAHLLNADAAELTIENGVVQVPGSATKKVTFAELGRIAYNNQHLIPDDMEAGLQ
                     ATYYYTFPHAKPNIVPGADRLVRAQFTFSAGAHAAIIEVDKATGKVEILRYLIVGDNG
                     TVINPDVVNGQVYGSAAHGIAVALGEGFIYSPEGQPLTVTYLDYGKCSTAETPKVEVI
                     HRPSPSPFTSLGQKAAGEGAAIPSPAAIASAVEDALTPFGVKITDLPLTPEVVWRLAN
                     DNPDSVRRSY"
     gene            complement(4089..4562)
                     /locus_tag="VF182_19490"
     CDS             complement(4089..4562)
                     /locus_tag="VF182_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6439312.1"
                     /translation="MKKTIRVTVNGRLYEEDVEPRILLSHFLRETLGLTGTHVGCVVG
                     ECGACSVLLDGKLIKSCLHFAVQVDGCSVTTIEGLAKDGQLSVIQEAFVKHYALQCGY
                     CTPGMVMASHAFLRDHSQPTEEEIRKALGGNLCMCTGYVQIVEAVKEAAKGSTNA"
     gene            complement(4596..5456)
                     /locus_tag="VF182_19495"
     CDS             complement(4596..5456)
                     /locus_tag="VF182_19495"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6439313.1"
                     /translation="MIRFDYLEPATLSEAFSLLEQHGEGARVIAGGTSLIIWMRQRLL
                     MPKVVIGLGRIPEFDTITFDPQGGLCIGAGARHRDIELSPVVKQHYPLLHETFRKVAQ
                     PRIRNMGTIGGNLAGGDPLTDPGASLIALDAELILASSNRTRTVSLEDFFVDYYQTAL
                     EPGELLTEIRVPPPQRPGWSHIKFTPRSIEDFATVGVAMTLRAKAGSCEDVRLALNSV
                     ASTIVRAKRAEDVLRGKRLADGVLREAGEIAATEVDPMDDNRGSADYKRDLVKVLVRR
                     AAEQAIDRLS"
     gene            complement(5453..7750)
                     /locus_tag="VF182_19500"
     CDS             complement(5453..7750)
                     /locus_tag="VF182_19500"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6439314.1"
                     /translation="MAKAEKFSVVGRRVQRVEGFDKVTGDSKFIADIFLPNMLVGKVL
                     RSPFPHARIRSIDTRKAEKLRGVRAVVTAEDTIKRPWGAFFADQYILSVGKTRYVGEE
                     VAAVAAIDPDIAEEAIDLIEVDWEPLPGVFDADEAMQEGAPLVHDDKPNNIAMTMDIE
                     RGNITQAFAESDVIVEDTFQSMPQWHCSIETIGSVAEYSPNGKYTIYMNTQTLFNARY
                     RIATALGVPETDVRIIQSAVGGGFGGKSCDDNNALVAAVLSRKAGKPVKLINTREEEF
                     LAGCRPRVFMKITVKLGFKKDGRIRAKHIKVIADNGAYSAKAPAITGVAAMRHDTCYK
                     YSDVRTEAHLVYTNKIPTGAFRGFGNPSAEWAVEQAIDLGAHELGIDPIEIARINAAE
                     TGYVSPHGNRVTSCELKQCLDMAEKMMDWKNQRVNKEPNTGLGLACTVHVSGKRHFGD
                     YDGSSATIKLNEDGKALILSGEGECGQGVHTAMCQIAAEELGLPVEDVEISRADTDLT
                     TFCLGAFASRLTYVSGNAVKNAAANVKQQLFQQASEMLEANPADLVANDGRIFVAGTE
                     GKSVSVADVARARLFRHNGAPIVGSGSFDADSVLPDSTRFGNESGAYNYGVQAAQVHV
                     DPETGQVEILNFVIASDCGTVIYPLGAEGQVEGGLSQGIGYALTEGLQIDEGKPVNPN
                     FSDYRIPSMRDMPPLQHAFAESYEPTGPFGAKGLGELNMDPTAAVISNAIFDAVGVRI
                     RTLPITPEKILRALQEKRAGESAKP"
     gene            complement(7848..8411)
                     /locus_tag="VF182_19505"
     CDS             complement(7848..8411)
                     /locus_tag="VF182_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6439315.1"
                     /translation="MKKQIQLNVNGEIHDVEIEANRLLLQVLRDNIGLTGTKEGCSIG
                     VCGACSVIIDGRVVSSCLTLAAGCAGKNIQTIEGLAKNGKLHPLQQAFIEYGGFQCGI
                     CTPGQIMAAKSLLDENPTPTEDEVKEWMSGNLCRCTGYYKILEAVMAVVDNKIDPDFA
                     FRKREQTAKIQSLDVYKAVAKTPHSQK"
     gene            complement(8607..10097)
                     /locus_tag="VF182_19510"
     CDS             complement(8607..10097)
                     /locus_tag="VF182_19510"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6439316.1"
                     /translation="MWLEGFLTALQPLNLLYLVAGTAFGMVIGALPGLGPLFGVALML
                     PLTFGMPAATAIIFLTAVHAATAYGDSFASIMINAPGGVGSVASCWDGHPMAQQGKAG
                     VAMGISTFGSFIGGVAGWISLVLLSPLLIMVAVRMGPPEYFMVSVMALSLLALASGEQ
                     PLKGLFLGGIGLLLSFVGRDPITAEARFTFGITYLEDGLPLAAVVLGLFALSQALVLS
                     QEKGSISQFRTIGNVWEGFLQVLKLPATVLRASIVGIFMGVLPALGLSSANVVAYFTE
                     KRAARDPEKFGQGDPRGLLAPEIAKNACIVGDLIPTFALGIPGSSVTAIFLAAMIMHG
                     LQPGAEFFSRSGVLPYTVFAGILLAQFSFFIMGLLFARQFAKAVLIPNALLVPIIVVL
                     SFIASLALRGNVEDIIVTFVFGFLGYLMSIYRYPAACVVLGLVLGDLVEANFHRSLLI
                     GRGSYLIFFTRPLSLSLFVLTVVMLLWPYLKFGSGAAQRDAGSTPA"
     gene            complement(10116..10559)
                     /locus_tag="VF182_19515"
     CDS             complement(10116..10559)
                     /locus_tag="VF182_19515"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX6439317.1"
                     /translation="MPQTLEGLLLVILAGFAALFLLVTKDYGTTAALFPRVVAAATLA
                     FLAFDLGWQYFLAKKKSAPVRHDAEVVSWWGALALQAGYIALIYIAGFSAATLAYLIA
                     CPWQLRYRRWAVTISHAVLLTFTIVYTFNSVFHVRLPKGLLGIPW"
     gene            complement(10570..11532)
                     /locus_tag="VF182_19520"
     CDS             complement(10570..11532)
                     /locus_tag="VF182_19520"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6439318.1"
                     /translation="MTYLKFMLLGFATCIGLMPANVFAQGIYPKEPIRLVITHAAGGT
                     TDMAARLIQPYLQKYLGVPIVIENMQGAGGNVARSFVFKQPADGYTLLVSQQPSMSSG
                     QIVSGGKFDVLKFVHVYNIAGRNYDCVALAPNSPFKTIDDMKKASAQQPLISAGTGVG
                     SNAYILAMLLRSKAGVNMTYVPFNSGTEAALALAGGQTQMGTGALDSYAPLHDQKRLR
                     VLAVAGPQRDESHKEFPTLAELGYDIKMDQMTGVLAPPGLPEQQLKILVAGFQKAFAD
                     KDYLAAADKAKQTLQPMPPAEFRKASEEMFTMIQSLESVLKQSK"
     gene            complement(11875..13533)
                     /locus_tag="VF182_19525"
     CDS             complement(11875..13533)
                     /locus_tag="VF182_19525"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6439319.1"
                     /translation="MNFAKPYEDLREFIAALDVHEKLYRIDREINKDTELQPLVRWQF
                     RGGIPESARRGFLFNNVTDGKKNKYNCRVLVGGLSGSEAIYSLGLKCQPDEVPDRWIY
                     ALDHLVPPIMVDHGPAQEEVHMGSELLAQGGINEFAVPISTPGFDNGPYITAGHWITK
                     DPETGQRNVGNYRGLIKGPTRTGLMTGTPQDLSNQWEKARRMGKPLEVAIVVGTIPVV
                     SYAATQKVPPDVDELALAGGLQGAPVQLVKCKTVDLEVPATSEIVLEGIIPTKYMEEE
                     GPYGESMGYIDPRTLSLVFELKCVTHRKSPIWVSIISQVTPSESSKIKAMGMATLIKR
                     SLVKKGFDSVQEVHLIEHLVNLRPYVAVSLKKRNDQEPWDVMQGILDYGDRVGKMIVA
                     VDEDINVKDPVAVIWAITHRSQPHKDFKIIGDRPFGATPIGMVATHPSSRYDNCESSV
                     LIDATRKADFPPLSLPKKEFMVRAKELWEELGLPKLEPQEPWHGYLMGYWPDELSNEA
                     DLAAKSEHEKVWEALQKTRVDVGEGDTMKTMRARWGKTHSGRAV"
     gene            13633..15045
                     /gene="cysS"
                     /locus_tag="VF182_19530"
     CDS             13633..15045
                     /gene="cysS"
                     /locus_tag="VF182_19530"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX6439320.1"
                     /translation="MSLKIFNTLSGEKEDFAPLIPGEVRMYVCGVTVYDSSHIGHARS
                     LLTFDTIYRYLRFSGYRVQFVRNFTDIDDKIINKANEQKSSCEAITERYIEEFHRDAE
                     TLGLLEPNLEPRATLHIVEIIELIQKLEKRQVAYRVDGDVFFSVQGFPGYGKLSRKRI
                     DELEAGARVEIDVRKKSPLDFALWKSSKPGEPSWESPWGPGRPGWHIECSAMSSKYLG
                     QPFDIHGGGRDLMFPHHENEIAQSEAAFGLPLARYWMHNGLLTVNGEKMSKSLGNYFT
                     IEQILQEHDPVAVRQLFLASHYRSPGDFSKEALQEAGRASERIYETIERIACSGIDRR
                     NRVADASLLANFRREMDDDFNTPRALALIFDEVRSVNRLLDAEQNDGVAQRASALETM
                     CETLGLLQDGIEPFLRRKKARWLRQQGFTDEEVQRAIAARDQARIERNWPEADRIRNE
                     LLAKGIIVEDSRAGTTWKVR"
     gene            15255..16832
                     /locus_tag="VF182_19535"
     CDS             15255..16832
                     /locus_tag="VF182_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439321.1"
                     /translation="MIQERGLAHGKFSQYVIVVQALNPQLKSASVLKVDDTVFIPLRP
                     DEVLAGPSSVASGGKTTNESFGNGSTTDYRVQRGDHLYQILREQLKITDEKELANYYA
                     LVKDLNPQRKEWDVLQEGELIRLPADKGGANVAQNASPLVTGSEDLTQSSEPIEPAQI
                     STAPRARLSLDHARRLLARENLPLLQQVLNTVGIEVESDGAEVFSFASGKVRMDKSSY
                     PIVLNRKLQQRVILDTGERLPASLRKNFESPDAGASVFSLPAAANLQDAVSQLLSRLG
                     FQALPNDRPIIIQQGDVAVELMGTWIAVGPEESGKAQEMFIITVTDQPDEVPEYLREQ
                     LSAKGVHLKEVVLASSSQAQITTHAAMRIAEEVKIWPREKFEKIDALLLAYGIPFGVA
                     EPESVEMGEGLRFETQIDRTFESKGQRIGLFFHQVQPEIKKALQQRRGMKVVELDLST
                     MSAKEIITRILRELRDETVYGEHKFSADSGNKDRVNITTAGFWLPNRSIFLTDRQIPP
                     SLHRFFFEKGIDLVYFD"
     gene            16952..17028
                     /locus_tag="VF182_19540"
     tRNA            16952..17028
                     /locus_tag="VF182_19540"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16986..16988,aa:Arg,seq:tcg)
BASE COUNT         3837 a         4696 c         4834 g         3903 t
ORIGIN      
        1 gatgtgtttt cgtgtcattg ctgattatag cgctcgagga aatgcgtcgg tttcgggaca
       61 atgaagcttc cggtgcataa agactgtttc agtctgttgg ccttcaaccg tacaccgttc
      121 tggtaccttc tttgctccac gagggctgat tatcgctaaa ccaatgattg atgggccaga
      181 aaagccggtg gaatctgact gtttatcttt acttggggtt ggttttcgcg tcggccgcac
      241 tacttaggta tagcgtgacg attattttcg aagctgtgat caacgggcgc taacgaaagg
      301 gagggtacgg gtttatgcag aaatctatcg tcgttggact tttcatttta cttacggcat
      361 cggtttcgtc acaagtgttg gctcaagaag atttcattga gaagagacag aaagcaatga
      421 agggcaacag cgcagatgtc aaagcgatta agggggcggt tgaaagcaaa gattatgcga
      481 ctatagaaac caaggccaag gacattatgg gcactgcaga taagattccc agcctgtttc
      541 ccaaaggaag caccaccgga aaaaccaaag ccaagcccgt gatctgggaa aaatccgacg
      601 agtttgcgaa gaatgccaag aatcttagta aggctgctgg tgaacttgcg accgcagcaa
      661 aatcgcagga tgagtcagcg gtgggaacca agttcaaagc gttaggcgaa gtctgctcct
      721 cttgccacaa ggaattccgg gccgacaaat attccgaatg attggtttag gtcgcgcgcg
      781 gcggtcagat catggccgcc gcccgctatc tagcactgta gcgctgcttc gatcgcccgc
      841 tcgacgtaaa cgtgcgtcag gtgagtgcgg tactcgctgg aaccgttgat atcttctatc
      901 acatcgatat tgcgcgtcga ttcggccgaa gcttccgcaa tgatctttgg ctcgagtttt
      961 ttccccgtca aaatttcttc acttcgactg ggtcgataag ctttatcagt aatgcctgtg
     1021 attcccagcg cagcgcgtgt gcaagttcct gtagaatcca ttgtcaaccg cacggccacg
     1081 cccactaccg caaagcctga cgaccggggt gcggccttca ggtacgctgt tttgtccgca
     1141 tccgtgaccg gaactcttat tgccgatacg atctcgtcgg gttcgagcac gctcatcaaa
     1201 agtcctagaa aaaaatcatc cgccttaatc caacgctcgc cactgggtcc cgccactttg
     1261 atttcggcat ccagcgccat gatcgctgca ggcatgtcgc cggcgggatc ggcatgggca
     1321 acgctgccgc cgatagtacc gcgatttcgg atctgaatgt cggcgatggt cgccgcggtt
     1381 tgtggcagaa gcgggcagcg tttctgaagc agttcggaat actcaatctg agcatgcgtg
     1441 accagcgcac cgagaacaag accattgcct tcctcgtcga tcatgtcgag tcccggaatc
     1501 ttgccgatat cgacgacata gccgggcttc gacagccgca tcttcatcag aggcagtaaa
     1561 ctttgtccgc cggatagaac ctttacatca tccctgtgcg ccgataggaa ctcaatggcc
     1621 tcagtaatcg atcggggcgc aaaatagtca aaagaaccag ggatcatgat tagtaggacc
     1681 tccgcacgga atcagggttg tcattggcca gacgccaaac cacttccggc gtgagcggca
     1741 ggtcagtaat cttgacgcca aaaggcgtga gcgcatcttc caccgcgctg gcaatggctg
     1801 ccggtgatgg aatagctgcg ccttctcccg ctgctttttg gccaagcgac gtgaacggtg
     1861 atggtgaggg tcgatggatt acctcgacct tcggagtctc cgccgtcgag catttaccgt
     1921 aatccagata ggtcaccgta agcggttggc cttccggact gtagataaag ccttcaccca
     1981 gggcaaccgc gatgccatgc gctgctgagc cgtacacttg gccattgacc acatccgggt
     2041 taatcacggt gccgttatcg ccaacaatta gataacgaag gatttcaact ttaccggttg
     2101 ctttatcgac ctctatgatg gcggcgtgag cgcccgccga aaaagtaaac tgcgcgcgca
     2161 ctaaacggtc ggcgcccgga acgatgttcg gcttggcatg aggaaaggtg tagtaatagg
     2221 ttgcttgcag tccggcttcc atgtcgtcgg gaattagatg ctgattgttg taggcgatgc
     2281 gccccaattc ggcgaatgtc actttcttgg tcgctgaacc cggcacctgg acaacgccgt
     2341 tttcgatagt caattccgct gcatcggcgt taagcaggtg cgcggccagc ttcaccacct
     2401 tctctcgtaa cttttttgcc gcgccgtgga tggcgccgat atcgtaaaga tgaaaattgt
     2461 tgccgctgtt ggcggccgcc acgccccaag gcgaaagatc actatcaaaa ggaatactcg
     2521 tgctgatctg atcaggactc gttcctaaca catcggcggc aacttgggct gtggttgttt
     2581 cgtgcgcctg gccacaattg ggcgagccca aatgtaacgc aatggtgccg tttttttcga
     2641 gcttgatggt ggcgccgttg accccgcccg atcccggcgg ctctttcatc tcaggaaaaa
     2701 tcgccatatc ccgggcggcg ttccggcccc cgggttcgac gccgacgacg atgccgatgc
     2761 cgattaaacg gcctttttca cgctcgcgtt tttgctcttc gcgtagctta tcgtaatcaa
     2821 ttttttccaa gagagttgac agcaagccgg gataatcgcc gctgtcgtag acgttgccgc
     2881 tgatcgtccg gtacggcatc tcacccggtt gaatcaaatt cttccggcgc acctcgatgg
     2941 gactcatgtt caagtcctgg gcgacacgat ccatcatccg ctcccagatg taacacatgc
     3001 cgggcttgcc gatgccgcga ttcggaatca ccgggcactt gttagtgact accgcaaagc
     3061 cttccatggc gatactgggc gtgcggtaag tattcgatag attgtttaac ttgttagtga
     3121 aatgaatcgt caaggtgctg atcgagccgc ccacatcgtc gtattcttta aagcgcaggc
     3181 cgagcacggt gccgtcattt ttaactgccg cctcggcctc gaactcctgg gcgcacgcgt
     3241 gtcccccagc catcatgtgc tcgctgcgat cctccaccca cttcaccggc ctgccggttt
     3301 tcatcgacag caaggctacc atgacgacgt acttgcggat caaatggatt ttttgtccga
     3361 aaccgccgcc aacatcggga atgatgacgc gcacgtcttg cagcccgagg gcctcacgca
     3421 aaccgtcgta aatcacttca ggcacctgcg ccgtaaccca tacggtcagt cttttgctgg
     3481 ccggatcgta ggatgcgatc acgacgaacg gctccagtgg cgtcgagctg taacgatgaa
     3541 tttttaagtt ttctttgaca acgcggtcgg ccgatttaaa tgcgccatcg atgtcgccgt
     3601 attggagcga gccatgccag gctagattac tgcccagctc gtcgaacagt gttgcggccg
     3661 aaggcttgat cgcttcacgc acgtcaacca ccggtcggag cggttcgtat tctactgaga
     3721 tgagctccag agcatcttcc gcagtgccgc ggtctcgagc agcgaccgcg ccgagaggct
     3781 cgccgacgta gcgggctttg tccacagcgc cggcatattc atcaatgggc cgtttgaggc
     3841 cggcggcgta gcgccccggc ttgaacggtt tggtgctttg cttgatatcg tcgggagtga
     3901 tcacggctat gacgttggga ttggccaggg cttgcgacgg atcgacagac agaattttgg
     3961 cgtgggcgtg cgggctgcgc aaaatcatcg cctgcagcat gccgggcaaa atgaagtcgt
     4021 cggtataaag gcctcgaccg gtgacatgtc gcggcccttc tttgctgcgt gtgcggctgc
     4081 cgatgaaatc aggcattggt ggatcctttt gccgcctctt ttacggcttc gactatctgc
     4141 acgtaaccgg tgcacatgca caaattgccg ccgagcgcct tacgtatttc ttcttcggtg
     4201 ggctgagaat gatcacgcaa aaacgcatgg cttgccatta ccattcccgg ggtgcaatag
     4261 ccgcactgca acgcataatg cttaacgaag gcttcttgaa ttacactcag ctgaccgtct
     4321 ttggccaatc cctcaatggt ggtcacactg cagccgtcga cttgcacggc gaaatggaga
     4381 caggatttaa tcagcttacc atcgagaaga accgagcacg cgccgcactc gccgacgacg
     4441 cagccgacgt gagttccggt caagccgagg gtttcccgca aaaagtgcga tagcaaaatg
     4501 cgcggctcga catcttcttc atataatcgt ccattaacgg tgacgcgtat cgtctttttc
     4561 atatggtttc aaactaacgt gtgctggaaa gcgggtcaac taagacggtc gatagcttgt
     4621 tcagcggcgc gccgcacgag cactttgaca agatcgcgct tgtaatcggc cgagccgcgg
     4681 ttatcgtcca tcgggtcgac ctcggttgcg gcaatctccc cggcttctcg gaggacaccg
     4741 tcagcgagcc gctttccgcg taagacatct tcggcgcgtt tggccctcac gatagtggaa
     4801 gccactgagt tgagcgccag ccgcacatct tcgcaggagc ccgccttagc ccgcaacgtc
     4861 atggccacgc cgacggttgc aaaatcttct atgctgcgag gggtgaactt gatgtgagac
     4921 cagcccggcc gttgaggagg tgggacgcgg atttccgtca atagttctcc tggctctagc
     4981 gcagtctggt agtagtcgac aaaaaaatcc tctaacgaga cggtcctcgt tcgattgctg
     5041 cttgccagga tgagttccgc atccagagct atcaggctgg cgcccgggtc tgtcaatgga
     5101 tcgccaccgg ctaaattgcc gccgatggtt cccatgttgc gaatcctggg ctgcgcgact
     5161 ttacggaagg tctcgtggag caggggataa tgctgcttca ccactggaga aagctcgatg
     5221 tcgcggtggc gcgcgccggc gccgatgcac agcccccctt gtgggtcgaa tgtaatggtg
     5281 tcgaattcag gaatacgccc gaggccgatt actaccttgg gcatcagcaa acgttggcgc
     5341 atccagatga tcagggaagt acccccggca atcacccggg cgccctctcc atgttgttcc
     5401 agaagtgaaa acgcttcgga tagagtggcc ggctccaagt agtcgaacct gatcatggct
     5461 tggcgctttc tccggcgcgt ttttcttgga gggcgcgaag aatcttctcc ggcgtgatcg
     5521 gcagcgtcct gatgcgcact ccgacggcat cgaagatggc attgctgatg accgccgctg
     5581 tcggatccat attcaactct cctaatcctt tggcgccaaa tgggccggtg ggctcgtagg
     5641 attcggcgaa tgcatgctgc agcggcggca tgtcccgcat cgatggaatg cggtaatcag
     5701 aaaagttcgg attgaccggt ttgccttcat cgatttgcaa accttccgtg agggcatagc
     5761 caatcccctg cgacaatccg ccttccactt gcccctcggc gcctagagga tagatgaccg
     5821 tgccgcagtc ggatgcgatg acaaagttta atatttcgac ttgcccggtt tccgggtcga
     5881 cgtgaacctg agccgcctga actccgtaat tgtaggcacc cgattcgttg ccgaaacgtg
     5941 tgctgtccgg caaaacggaa tcggcatcga agctgccgga gccgacgatg ggcgcgccgt
     6001 tatggcgaaa caggcgtgct cgagcgacat cggcgacgga caccgatttc ccttcagttc
     6061 ctgcgacaaa gatgcggccg tcgttggcaa cgaggtctgc gggattggct tcgagcattt
     6121 cagatgcttg ttggaaaagt tgctgcttca cattggcagc ggcgttcttt accgcattgc
     6181 cgctgacgta ggtaagccgg ctggcaaaag cgcctagaca gaacgtggtc aaatcagtgt
     6241 cggcgcgtga gatctcaacg tcttcgaccg gcaggcccaa ttcttcagcg gcaatctggc
     6301 acatcgcggt atggactccc tggccgcatt cgccttcgcc gctgaggatc aacgccttgc
     6361 cgtcttcgtt cagcttgatc gttgcggaac tgccgtcata atcgccgaag tgacgcttgc
     6421 cgcttacgtg aaccgtgcat gctagcccca gaccggtatt cggctccttg ttgacgcgct
     6481 ggtttttcca gtccatcatt ttttccgcca tgtcgaggca ctgctttaac tcgcagcttg
     6541 tgaccctatt gccgtgtggc gatacatacc cggtctcggc cgcattgata cgggcgattt
     6601 cgatcggatc gatgccaagc tcatgagcgc ctaaatcgat cgcctgctcg accgcccatt
     6661 cggccgacgg attgccgaat ccgcggaacg cgccggtcgg aatcttgttg gtataaacca
     6721 ggtgagcttc ggttctgacg tccgaatatt tgtaacaggt gtcatgccgc atcgccgcta
     6781 ccccggtgat tgccggcgct ttcgccgaat aggcgccgtt gtcggcgatc actttgatat
     6841 gcttggcgcg aatgcgaccg tcctttttaa accccaactt gacagtgatc ttcatgaata
     6901 cccggggccg gcaaccggct aagaactctt cctcacgcgt gttgataagt tttacgggtt
     6961 tgccggcttt gcgcgataga accgcggcca ccagtgcatt gttgtcgtcg caggacttgc
     7021 cgccgaagcc gccgcccacc gcgctctgta tgatccggac atcggtctcg ggcacgccga
     7081 gagccgtggc aattcggtat cgcgcgttaa acaaggtttg agtgttcatg tagatggtgt
     7141 atttgccgtt aggcgaatat tcggcaacgc taccgatggt ctcgatcgag cagtgccatt
     7201 gcggcatgct ctggaatgta tcctcgacga tgacatcgga ctcagcgaac gcttgggtga
     7261 tattgccgcg ctcgatatcc atggtcattg caatattatt gggcttgtca tcatgcacca
     7321 gcggcgcgcc ctcttgcatc gcttcgtcgg cgtcgaagac ccctggcagc ggttcccaat
     7381 cgacctcgat cagatcaatg gcctcttcgg cgatgtcggg atctatcgcg gcgacggcgg
     7441 cgacttcttc gccaacgtac cgagtctttc cgaccgaaag aatatactgg tcggcaaaaa
     7501 acgcgcccca cggtcgtttg atggtatctt cagccgtgac cacagcgcgc acgccgcgca
     7561 gtttctccgc cttgcgcgtg tcgatgctgc ggatccgcgc atgcggaaac ggactgcgca
     7621 gcactttccc caccagcata ttgggaagaa aaatatcagc gatgaacttc gaatctccgg
     7681 tgactttgtc gaagccttcg accctttgaa cgcgccggcc gaccaccgag aacttttctg
     7741 cttttgccat aactacaatc tcttttagtt tgagtactat gttcgggtgc gaccccgatg
     7801 agtcatttcg ctcatcccca accttggtcg cgctaaggtc gcctgactca cttttgtgaa
     7861 tgaggcgttt ttgcgaccgc cttgtaaacg tccaggcttt gaatttttgc cgtctgctcg
     7921 cgtttgcgaa atgcgaaatc gggatcgatc ttgttatcga ccaccgccat caccgcttca
     7981 agaattttat agtagccggt gcagcggcaa aggttgcccg acatccattc cttgacttcg
     8041 tcttctgttg gcgtggggtt ttcatccagt agagacttcg ccgccatgat ctgtccggga
     8101 gtgcagatgc cgcattgaaa gccgccgtac tcgataaacg cctgctgcag cggatggagc
     8161 ttgccgttct tggcgagacc ttcgatggtt tggatatttt ttcccgcgca gcccgcagcc
     8221 aacgtcaggc acgagcttac gaccctgccg tcgatgatga cactgcatgc gccgcagacg
     8281 ccgatggagc agccttcttt ggtcccggtc aaaccgatgt tgtcgcgcag aacttgcagc
     8341 agcagccgat tggcttcgat ttcaacgtcg tggatctcgc cgttaacatt taactgaatc
     8401 tgctttttca ttttttttaa aatataggcc aagctaccga gtgtcaacca acggtggatc
     8461 gggtatcgct gcagtcaaag tacaatgcaa atcttcactt cgaaagggat gctcgctcgc
     8521 gctcgaaatg acagcaaaac aaatgatgcg gtattttcaa agcctcagag ataatgttgc
     8581 cgggagtgaa cgccccgatc tcgcccctac gccggagtgc tgccggcatc gcgttgtgcc
     8641 gcgccggagc cgaacttcaa atacggccag agcagcatga ccacagttag aacaaacaaa
     8701 ctaagcgaca gtggacgcgt aaaaaagatc agataggatc ctcggccgat caacaaagag
     8761 cgatgaaaat tggcctcgac cagatcgccg agaaccaggc ccaaaacgac gcaagctgcg
     8821 ggataacggt aaatcgacat caaatagccg agaaagccga aaacaaaagt gacgatgata
     8881 tcctcgacgt tgccgcgcag cgcaagagaa gcgatgaagc tcaacacgac gatgatcggc
     8941 accagcagag cattgggaat aagcacggct ttggcgaatt ggcgcgcgaa cagcagcccc
     9001 atgatgaaaa aagaaaattg ggcgagaaga attcccgcga aaacggtgta aggcaatacg
     9061 cccgagcgcg aaaagaactc ggcgccgggt tgcaggccgt gcatgatcat ggcggcgaga
     9121 aaaattgccg tgaccgaaga gccgggaatg ccgagggcga acgtgggtat gagatcgccg
     9181 acgatgcagg cattcttcgc aatttccggc gccagcagac ctcgagggtc gccctggccg
     9241 aacttctccg gatctctcgc cgcgcgcttc tcggtgaaat aggcgacgac gttggccgag
     9301 gacaatccga gagcgggcaa tacgcccatg aaaatgccga cgatgctggc gcggaggacg
     9361 gtcgcgggta gtttcaaaac ctgaaggaag ccctcccaga cattaccgat cgttctaaac
     9421 tgcgagatgc tgcctttctc ctgagagagc acgagggctt gggaaagtgc gaacaggccg
     9481 agaacgacgg ctgcaagcgg cagcccgtct tcgagataag taataccgaa agtaaagcgg
     9541 gcttccgccg tgatcgggtc gcggccgacg aacgacagca gcaggccgat gccgccgaga
     9601 aacagccctt tgaggggctg ctctcccgaa gccagcgcca gcaacgaaag cgccatgacc
     9661 gagaccatga aatattcggg aggacccatg cgcacagcga ccatgatcaa caaaggagac
     9721 agcaggacga gcgagatcca cccggccacg ccgccgatga aggagccgaa agtggaaata
     9781 cccatcgcca caccggcctt gccttgctgc gccatcggat ggccatccca acaagaggcg
     9841 acggagccta cgccgccggg cgcgttgatc atgatcgagg cgaaggagtc gccgtaggcg
     9901 gtcgcggcat gaacggcggt gagaaaaata atcgccgttg cggccggcat gccgaaagtt
     9961 agcggcagca tgagcgcaac cccgaacaag gggccgagtc ccggcaacgc gccgatcacc
    10021 atgccgaacg cggtgccggc gacgagataa agcagattca acggctgtaa tgcggtcaga
    10081 aagccttcaa gccacatcgt tgatctttcc gtcggttacc agggaatccc cagcagacct
    10141 ttaggcaggc ggacatggaa cacggaattg aaagtgtaaa cgatggtgaa cgtgagcaat
    10201 acggcgtgcg agatcgttac ggcccacctc cgatagcgga gctgccacgg gcaggcaatc
    10261 aagtaagcga gcgtcgccgc cgaaaacccc gcgatgtaaa tgagcgcgat gtagccggcc
    10321 tgaagcgcca acgcccccca ccaggaaacg acctccgcgt catggcgcac cggcgcggat
    10381 ttttttttag ccaggaaata ttgccagccg agatcgaaag cgagaaacgc cagggtggcc
    10441 gccgcaacaa cgcggggaaa caacgcggcc gttgttccgt aatcttttgt aaccagcagg
    10501 aataaggcgg cgaaccctgc caggatgacg agcaaaagcc cttccaaggt ctgcggcacg
    10561 ttgttcaggc tacttggatt gtttgagcac ggattccaaa ctttggatca ttgtgaacat
    10621 ttcttccgac gctttgcgga attcggccgg cggcatgggc tgcaacgtct gcttcgcctt
    10681 atcggccgcc gccagatagt ccttgtcggc gaatgccttc tggaacccgg cgacgaggat
    10741 cttcaattgc tgctccggca aacccggagg agccaagaca ccggtcattt gatccatctt
    10801 gatgtcatag cccagctcgg cgagagtggg aaactctttg tgcgactcat ccctttgcgg
    10861 tccggcaaca gcgagaacac gcaggcgttt ctgatcgtgc agaggcgcgt agctatcgag
    10921 cgcgccggtg cccatttgcg tttgcccacc ggcgagagcc agcgcggcct cggtgccgct
    10981 gttgaacggg acataagtca tattcacgcc ggctttcgat ctgagcagca tggcgagaat
    11041 atacgcattg ctgccgacgc cggtgccggc tgagatcagc ggttgctgcg ccgacgcttt
    11101 cttcatatcg tcgatcgtct tgaagggcga attcggcgcc agtgcaacac agtcgtagtt
    11161 gcgcccggcg atgttgtaaa catgtacaaa tttcaggacg tcgaattttc ccccggagac
    11221 aatctggccg ctcgacatcg agggctgctg gctgaccagc agcgtgtaac catccgccgg
    11281 ctgcttgaat acgaacgatc gcgccacgtt gccgccggcg ccctgcatgt tttcgatgac
    11341 gatgggaacg cccagatatt tctgcaagta gggttgaatg agccgcgccg ccatgtcggt
    11401 cgtaccgccg gcggcgtgag ttatcacgag acgaataggt tctttcgggt agatcccctg
    11461 ggcaaaaacg ttcgcgggca tgagcccgat gcatgttgcg aaccctaaca gcatgaattt
    11521 taaatatgtc atttagtttc aacccttctt gttggcactc ctacgctgag caattagcgt
    11581 accggcaggt tgtcagtggt tttacacggc tccgacctaa attgtttgca aacgtgagaa
    11641 aggcagtccg tttgattcca aagtttaata cggaatcagg gaaggtaaat gctatttgag
    11701 cgtacgcccg gcggcgcgag ggataaactc agatgcgctg ctaaaacgct gcgacttgac
    11761 aatggatttg aatcaacagc tatttcggag atcgtgaatt acgcctatgt tcgtcgcaac
    11821 agccctgtca tacaaacggg gtggaagctt tttccgcgtc attgattgtc aattttatac
    11881 tgcgcggccc gaatgggttt tgccccaacg tgcgcgcatg gttttcatcg tgtcgccttc
    11941 gccgacatcg acgcgggtct tttgcagcgc ttcccagacc ttttcatgtt cgctcttggc
    12001 tgccaggtcg gcttcgttcg ataactcgtc cggccagtat cccatcaagt agccatgcca
    12061 gggttcctgc ggttcgagct tcggcaagcc gagctcttcc cacagttctt tggcgcgcac
    12121 catgaattct ttcttgggca atgacagcgg cggaaagtca gctttgcgcg tggcgtcgat
    12181 caagaccgac gattcgcagt tgtcgtagcg gctcgacgga tgcgtcgcta ccatgccgat
    12241 cggcgtggcg ccgaacgggc ggtcgccgat aatcttgaaa tctttgtgtg gctgggaacg
    12301 atgggtgatc gcccagatga ccgctaccgg atctttcacg ttgatgtctt cgtcgacggc
    12361 gacgatcatt ttaccgacgc gatcgccgta gtcgagaata ccctgcatga catcccaggg
    12421 ttcctggtcg ttgcgctttt tgaggctaac cgcgacatag ggccgcagat tgacgagatg
    12481 ctcgatgaga tgaacctcct gcaccgagtc gaaacctttc ttgaccagag aacgcttgat
    12541 caaggtcgcc atacccatcg ccttgatctt cgagctttcg ctcggcgtga cctggctgat
    12601 gatcgaaacc cagatcgggc tcttgcgatg agtaacgcac ttgagctcga acaccagact
    12661 cagcgtgcgc ggatcaatgt agcccattga ctcgccgtag ggtccttctt cttccatata
    12721 ctttgtcggg atgatgcctt cgagcacgat ctcggacgtg gccgggactt cgaggtcgac
    12781 ggtcttgcat ttgactaact gtaccggcgc cccctgcaga ccgccggcga gcgccagctc
    12841 gtcgacatcg ggcgggactt tttgcgttgc cgcgtaggag actacaggga tggtgccgac
    12901 gacgatggcg acttccaacg gtttgcccat gcggcgagct ttttcccatt gattcgagag
    12961 atcctgcggt gtgccggtca taaggccggt gcgcgtcggg cccttgatca acccgcggta
    13021 gttgccgaca ttgcgctggc cggtttccgg atctttggta atccaatggc ccgcggtgat
    13081 gtaggggccg ttgtcgaatc ccggcgttga aatcggaacg gcgaattcat tgatcccgcc
    13141 ctgggcgagc aattcagacc ccatgtggac ttcttcctgc gccggcccat ggtcgaccat
    13201 tatcggcggc acgagatgat ccagcgcgta gatccagcgg tcgggcacct cgtccggctg
    13261 gcatttcaag cccagcgaat aaatcgcctc ggagcccgac agcccgccga cgagcacgcg
    13321 gcaattgtat ttgtttttct tgccgtcggt gacattgttg aacaagaaac cgcgccgggc
    13381 cgattcggga atgccgccgc gaaattgcca gcgaaccagc ggctggagct cggtgtcttt
    13441 gttgatctcg cggtcgatcc gatagagttt ttcatgaacg tcgagcgcgg cgataaactc
    13501 tcttaagtcc tcataaggtt tagcaaaatt catagatttt ttgtctatgc tttttccctc
    13561 gcattagcaa gcggcctgcg cgaaactgct tgcaaatccg gatctgatcc gtaacattga
    13621 gaattcgttc atatgtctct caaaattttc aacacactca gcggggaaaa agaagatttt
    13681 gcgccactca tcccgggaga agtgcggatg tatgtgtgcg gcgtgacggt ttacgattct
    13741 tcgcatatcg gccatgcgcg ctcactgctg accttcgata ccatctaccg ttatcttcga
    13801 ttttccggtt atcgcgtgca gttcgtgcgt aacttcaccg acatcgacga taagataatc
    13861 aacaaagcta acgaacaaaa aagtagctgc gaagccatca ccgagcgcta tattgaagaa
    13921 tttcatcgcg acgccgaaac tttaggatta ctggagccga acctcgagcc acgagcgacc
    13981 ctccatatcg tagagatcat cgagcttata caaaaactgg agaagcggca ggtggcctac
    14041 cgggttgatg gcgatgtctt tttttcggtt cagggtttcc ccgggtatgg caagctctcg
    14101 cgcaaaagga tcgacgagtt ggaggcggga gctcgcgtcg agatcgatgt gcgcaagaaa
    14161 tcaccgctgg atttcgctct ctggaagtcg agtaagcctg gcgagcctag ctgggaaagt
    14221 ccctggggtc caggccggcc gggctggcac atcgagtgtt cggccatgag cagcaaatat
    14281 ctcggccagc ctttcgatat ccacggcggc ggcagagatt tgatgtttcc gcatcacgaa
    14341 aatgaaatag cccagtccga ggccgcattc ggcttgccgc ttgcgcgcta ctggatgcat
    14401 aatggtctgc tgacggtaaa cggcgagaag atgtcaaaat cgctaggcaa ctactttacg
    14461 atagagcaaa ttctacagga gcacgatccg gtcgcggtgc gtcagttgtt cttggccagc
    14521 cattaccgaa gtccaggcga tttctccaag gaagcattgc aggaagcggg tcgagcgtcc
    14581 gagcgaatct acgaaacgat tgagcgaatt gcgtgttccg gaatcgatcg acggaacagg
    14641 gttgcggacg cttcattgct ggctaacttc cgtcgagaga tggatgatga cttcaatact
    14701 ccgcgagcgt tggctttgat ctttgacgag gttcgatctg taaaccggct gcttgatgcc
    14761 gagcagaatg acggtgtggc tcagcgcgct tcggcactgg agacgatgtg tgaaactctt
    14821 ggactattgc aagatgggat agagccattt cttaggagaa agaaggcacg ttggctgcgg
    14881 cagcaaggct ttaccgacga ggaggtgcag agagctatcg ctgccaggga tcaggcccga
    14941 atcgaaagaa attggccgga ggcggatcgt atccgcaatg aactactcgc caaaggaatc
    15001 atcgtggaag actctcgagc cggtactact tggaaggtga gatgaagtat cgcccgtccg
    15061 ataaaacccc ggcgctcctt gcgatcacgt taacaacggc aatagttgtc gcccacgtct
    15121 tcgcgcaaga aaaatccgac agagctgaat cgcagctgac cctgcgtaaa tctgcggaga
    15181 ctcgggagta caaaggaact accatccaag gggaaaatcg ccggatagtc cccggcgatt
    15241 cgctgtggcg catcttgatt caagaacgcg gactggctca tggaaaattc agccagtatg
    15301 tcattgtcgt tcaggctctc aatcctcagt taaaaagcgc cagcgtgctc aaggtcgatg
    15361 acactgtgtt tattccgtta cgaccagacg aagttcttgc tggaccgtcc agtgttgcga
    15421 gtggtgggaa gacgacgaac gagtcctttg gcaacggctc gacaactgat tatcgggtgc
    15481 aacggggcga tcatctatat cagatcctgc gtgagcagtt aaaaataacc gacgagaaag
    15541 aactcgcgaa ttactacgcg ctggttaaag acttgaaccc acagcgtaag gaatgggatg
    15601 ttttgcagga aggggagttg atccgacttc ctgcggataa aggcggagcc aacgtagcgc
    15661 agaacgcatc accgttggtg acgggatctg aagatctaac tcaatcctcc gaaccgatcg
    15721 agcccgccca gatttcaact gctccgcggg cgcgcctgtc cttggatcac gcgcgacgac
    15781 tcctcgcgcg agaaaacctg ccgttattgc aacaagtgct gaatacggtt gggattgaag
    15841 tcgagagtga tggggctgag gtgtttagtt ttgcaagcgg caaggttcga atggataaaa
    15901 gttcttaccc gattgtcttg aaccgcaagc tgcagcaacg agtgattctt gatacaggtg
    15961 agcgattgcc ggcttcattg cggaaaaatt ttgagtctcc agatgccggt gcatcggttt
    16021 tttctcttcc ggcggccgcc aatcttcaag acgcggtcag ccaattatta tcccgcctgg
    16081 gatttcaggc gttgccaaac gatcgcccca tcatcatcca gcagggtgat gttgcggttg
    16141 aactcatggg aacatggatt gctgttggcc ctgaagagag cggcaaagct caggaaatgt
    16201 tcatcatcac agtcactgat caaccagatg aagttcccga atacctgcgc gagcaattgt
    16261 cggcgaaagg agttcatctg aaggaagtcg tcttggcctc ttcttctcaa gcgcaaataa
    16321 ccacccatgc ggcgatgaga atagcggagg aggttaagat ttggccgcga gaaaagttcg
    16381 agaaaattga tgctttgctg ctcgcttatg gaattccctt tggcgtggcg gagccggagt
    16441 cggtcgaaat gggggagggg cttagatttg agactcagat cgatcgcacc ttcgaatcta
    16501 aaggacagcg aatcggactc ttctttcacc aagtgcaacc tgaaataaag aaagcattgc
    16561 aacagcgacg aggaatgaag gtcgttgaac ttgatttatc gaccatgtca gcaaaggaga
    16621 tcatcactcg catactacgg gaactgcgcg acgaaaccgt ttacggcgaa cataagtttt
    16681 cagcggatag cggcaacaaa gatagggtta atataaccac agcaggattt tggttgccaa
    16741 atcgttccat atttctcacc gaccgtcaga tcccgccatc cttgcatcgg ttcttttttg
    16801 aaaaaggtat agatttggtt tactttgact agtggctgac gcaacgatgt gacggttcat
    16861 gcgaccagcg ggcggctcct ggtcggggat ctgggcgctg ttgaagagcg ccggcctaga
    16921 tccagccaag attactcata tcgatctaac ggcgcccgta gctcaactgg acagagcatc
    16981 agacttcgga tctgagggtt gggggttcga atccctccgg gcgcgccatc gtttttaaac
    17041 ttactttccg aaagtccgat cttctttaca gttgtctcga ctggtgattc caatcttgtt
    17101 tctgaggggt gcccggtagc gcagtcgtcc agcttattga cgatcgccgt cctgtaccga
    17161 gtgctcatag atgtctacag tcttgttact tgagctgtgg cctacctgat ccttcacatg
    17221 gttcaaggct catgcactgt gcgctcccta actagttgta gaactccgcc
//
LOCUS       DASUVS010000365         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1465514, whole genome shotgun sequence.
ACCESSION   DASUVS010000365 DASUVS010000000
VERSION     DASUVS010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465514"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VF182_19545"
     CDS             complement(<1..371)
                     /locus_tag="VF182_19545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6439322.1"
                     /translation="MADATTVLLIDADRAYRDYYAQRLYASTIKYDVFQAATGRSGLD
                     IIARQPIDCVVLEIDLPDMSGVEVMANSIPHPYRPERAIIVLTRFYNQLLANLAMKNG
                     AQAAFPKTAESGELLEASIVKA"
     gene            complement(852..1199)
                     /locus_tag="VF182_19550"
     CDS             complement(852..1199)
                     /locus_tag="VF182_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439323.1"
                     /translation="MGFKFPQFATHFTIYQTRIRFQAPLYRREINRKQLTAIAVVYQI
                     QETKTVVVGYRSAVYACQGRMLGIRQPLDGPVFEKLGHAEYWCWNAMLDHYERGLGMS
                     DARIEAFKGMVDF"
     gene            complement(1371..1447)
                     /locus_tag="VF182_19555"
     tRNA            complement(1371..1447)
                     /locus_tag="VF182_19555"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1411..1413),aa:Pro,seq:tgg)
     gene            complement(1438..2046)
                     /locus_tag="VF182_19560"
     CDS             complement(1438..2046)
                     /locus_tag="VF182_19560"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HEX6439324.1"
                     /translation="MLRDLVLATRNRHKGAELAAILAGLPVCIRTMDEFPDVPDVIED
                     GETCEANAVKKARAVAEATGLLAVADDTGLEVDALGGRPGVYAARYAGPQATYEDNCR
                     KLLKELAGVPRDRRTARFVTVAAVAEPSEKVQVTTGRLDGLITEAQAGEQGFGYDPVF
                     YVPDLGKTLAELSTEQKNRISHRAKAFAQVRELLQNRLRLGA"
     gene            complement(2052..>2487)
                     /gene="rph"
                     /locus_tag="VF182_19565"
     CDS             complement(2052..>2487)
                     /gene="rph"
                     /locus_tag="VF182_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX6439325.1"
                     /translation="ALRAVTDLPQLGERSIWIDCDVIQADGGTRTAAITGSFIALADA
                     LAILKTRGLLKKRPLTDYLAAISVGKVGGEILVDLAYLEDSTAEVDLNLVMTGKGQFV
                     EIQGTAEKTPFAKQDMDGFLAQGWGAIQTLTAMQRDLIGALD"
BASE COUNT          546 a          666 c          700 g          575 t
ORIGIN      
        1 gctttaacga tggaggcttc aagtagttct ccgctttccg ctgtcttcgg gaatgcggct
       61 tgtgcgccat tcttcatcgc aaggtttgcg agcaattgat tgtagaatcg tgtcaagacg
      121 atgatggccc tctcagggcg gtaagggtgt ggaatcgaat ttgccataac ctcaaccccc
      181 gacatatcag gcagatcaat ctcaagcacc acacaatcaa tcggttgccg cgcgataatg
      241 tcgagtcctg atcggccagt ggccgcctgg aacacatcgt actttatcgt ggatgcataa
      301 aggcgctgcg cgtagtaatc gcggtaagcg cggtcggcat cgataagaag aacggtagtc
      361 gcgtcagcca cgacagtcag cttttgtcgg caaatggcgt gaattgccga tgtgagcctc
      421 cgtgggaaaa atatcccaaa gtgcgatggc agtttaaaat tgagagtcat gtttggtcaa
      481 ggtagtattt tggctctgtg aaaattacgt agcaaggtgc cacgatgttc tacaatcatt
      541 tcatctgggc caaatggctc catggataac gacaatagta agagagaagg gcgggatgac
      601 ttgtctaatg ggcggggtca cttacgcggt cttctaagtc cctttagaaa tgacatccgc
      661 ataagcaacg atctgaattt ccgaaagaac attcgcaatg gcggccaaca gcacaggcat
      721 ccgagacgtg ggtgactttg ctaggtaagg cgtatttgtt taagagaaga cccaacgctc
      781 caacgatgag aacggcaatc agtaccgtga agttcatgac gttcgaccca gaggggcgac
      841 gaaaagcgtc cttagaagtc caccatgccc ttgaacgctt cgatccgtgc gtcagacatg
      901 ccaagaccgc gttcgtaatg atcgagcatg gcgttccaac accagtactc ggcatgtccc
      961 aatttctcga ataccggccc gtcgagcggt tgacggatgc cgagcatgcg gccttggcag
     1021 gcatacactg ctgagcgata gccgactaca acggttttgg tttcctggat ttgatacacg
     1081 actgcgatag cagttagctg ttttctattg atttcccgtc gatacagtgg ggcctgaaaa
     1141 cgaattcgtg tttgatagat cgtgaaatga gtggcgaact gagggaactt gaatcccaaa
     1201 aattcagaac ctattgggcc ccacttgcaa ccagcgtgtt ggcgtcggcg aacactgctc
     1261 cggctcgggc caagactcat aggcgccttt cctggcgcca gcaagaaact tcttatcgcg
     1321 gtagatttac gagttcttgc gaatcaaaca aacatttata agacattgat tggtcggggc
     1381 gagaggattt gaacctccga catcctgctc ccaaagcagg cgcgctaccg ggctgcgcta
     1441 cgccccgagc cgaagtctat tctgcaacaa ctctcgcact tgtgcaaagg ctttcgcacg
     1501 atggctgatg cggtttttct gctcggttga gagttccgcc aaggtttttc ccaaatcggg
     1561 cacatagaat acaggatcgt agccgaaccc ctgctctccc gcctgtgcct ctgtgatcag
     1621 gccatcgagt cggccggtgg tcacttggac cttttctgat ggttccgcca ctgccgcgac
     1681 cgtgacaaag cgcgcggttc ggcggtcgcg cggcacgccg gctaattcct tcagcaattt
     1741 gcgacagttg tcctcgtatg ttgcttgcgg gccggcataa cgggcggcat agacgcctgg
     1801 gcgacctccg agagcatcca cttcgagtcc cgtgtcgtcg gcgacggcga ggagtcctgt
     1861 ggcctcggcg acggcgcggg cttttttaac ggcgttcgct tcgcaggtct cgccatcttc
     1921 gatgacgtca ggtacgtcgg ggaattcatc catcgtgcgg atgcagaccg gcagaccggc
     1981 gagaatcgcg gccagttcgg cgcccttgtg ccggttgcgg gtggcaagaa ccagatcgcg
     2041 aagcatcggg gctagtccaa cgctccgatc agatcgcgct gcatcgcggt gagtgtttgg
     2101 atggctcccc agccctgggc aagaaatccg tccatgtctt gtttcgcgaa cggtgttttt
     2161 tccgccgttc cttgaatctc gacgaattgc cctttgccgg tcatgaccaa gttgagatcg
     2221 acttcggcgg tcgaatcttc aaggtaggcc aggtctacga ggatctcccc gccgaccttg
     2281 ccgacgctga ttgcggcgag atagtctgtt aatggacgct ttttaagcaa gccgcgcgtc
     2341 ttcaagattg ccagcgcgtc cgcaagcgcg ataaagctac cggtgatcgc ggcagttcgg
     2401 gtgccgccgt ccgcctggat gacgtcgcaa tcaatccaga tggatcgttc tcccagctgc
     2461 ggaagatcgg tcaccgcccg caatgcc
//
LOCUS       DASUVS010000366         6299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1684495, whole genome shotgun sequence.
ACCESSION   DASUVS010000366 DASUVS010000000
VERSION     DASUVS010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684495"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VF182_19570"
     CDS             complement(<1..522)
                     /locus_tag="VF182_19570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439326.1"
                     /translation="MARNGFKILDSDMHVFEPHDLYLKYMDPKWGDRIPRGEPRKRHG
                     QIKFAYADGKPIRPSGTQILTTTSPKTDAAESAPGEAQVAHRYRNPLKRDYDAISQLD
                     AMDEEGLDVAVLFRTFPLHCDDSLEAEYANDLCRAWNNWMADFCKADPKRLRPSALIT
                     LHDVDLAVDETRRA"
     gene            complement(560..1552)
                     /locus_tag="VF182_19575"
     CDS             complement(560..1552)
                     /locus_tag="VF182_19575"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439327.1"
                     /translation="MSQLVRAAWAFFLVVVFPSLGAAQKLVVGNTAISAEGLPIWFAH
                     ETGIYKKNGLDVQPVFLGGGGPRSLSVILSGECPISHGSGLPLINSNIGGADLVMIAG
                     GTTTINYWLVARPETKSPEQLRGGAIAIGSFGTTTDFAARFALRKFGLVAGKDVTLLP
                     VGNNPIRLSALETGRVVATPLNPPASVMAQKKGLNILLDISTLGIQWPHTGAITTRKF
                     IRENTDTVRRFVKSYIEAVHRIKTDRETGMKVLAKYFKGLQDREVLVKTYDLSFSEAV
                     MPRKQYPTLDGIKAILEYVAENDPRSKKAKPEDFVDLRFVKELDDSGYTESLYQ"
     gene            complement(1908..2999)
                     /gene="dinB"
                     /locus_tag="VF182_19580"
     CDS             complement(1908..2999)
                     /gene="dinB"
                     /locus_tag="VF182_19580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX6439328.1"
                     /translation="MRIIVHVDMDAFYAAIEERCNPALRGLPVVVGADPKDGRGRGVV
                     TTANYRARDFGIRSALPISRAWRLAQAACRAGEPETIFVRSNFQLYEIVSGRIMRIFE
                     RFSDAIEEASIDEAYLDVSSLKSFDLARVNMIALKNEILEQEGLGCSIGIGPNKLIGK
                     IASGRDKPNGLTVIMPHEVQEFLDPLPIRVIPGIGPKSEAFLQQQSVRLVKELRQIPQ
                     ATLADWFGSWGLRLFEKAHGIDESQVSNEWTRKSVGEQETFERDSRDLALVSAQLDKM
                     AERVVAKLRRNGFSGFRTLTLTVRFSDFDTRNRSRSIKNGILLDSNGDALQRIQNEAR
                     ELLLPFFDARENPRGKAIRLIGLRLEKLF"
     gene            3098..3565
                     /locus_tag="VF182_19585"
     CDS             3098..3565
                     /locus_tag="VF182_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009555662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX6439329.1"
                     /translation="MARKESAGIVLYRYCDSKLEIFLVHPGGPFWKNKDAGAWSIPKG
                     EFERGEDPLEAAKREFHEETGYSLDGRFIPLEPVRQPGGKVVHAWALEGNCEAESIKS
                     NSFMMEWPPRSGRRQEFLEVDRAGWFSLEPARQKILKGQLMLLDELQRILQSP"
     gene            4172..4492
                     /locus_tag="VF182_19590"
     CDS             4172..4492
                     /locus_tag="VF182_19590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439330.1"
                     /translation="MKLFLVLRQETAGQCSIVAARSKRDAINKITRLEGVDDAWAIRI
                     EEFMITFKMEENIAFFQPDLDRAVMSMNIANQRGSRGRIFRLKGDAVMKKPKIANETA
                     IAIH"
     gene            4955..5284
                     /locus_tag="VF182_19595"
     CDS             4955..5284
                     /locus_tag="VF182_19595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439331.1"
                     /translation="MNIARLRKADETSYLFKPVTGRSPVWQIPKRHGPENTFFHRLAP
                     AFTAPPYTKDHEEKLPTQDLFWVDMIVNALIVIVIASASYFFISAKFLQTIDPAISPY
                     SERSPSR"
     gene            complement(5488..>6299)
                     /locus_tag="VF182_19600"
     CDS             complement(5488..>6299)
                     /locus_tag="VF182_19600"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX6439332.1"
                     /translation="LLSSYKWWLLARSGSIEAPWLLAARAYFLGMAVNCFGLGTVGGD
                     LARALVLGSKSKQKTTALASVFADRLHGMTVLAAIGIVSMALFGRQQIGGEFLALLAA
                     CIGAVVLAWYFGPALALAVLPKQGALHKNVEKIAQVFPRDLATISYISLISLVFHLLQ
                     IFLHQVMAAGFGIQIPWPVLLVTIPVINILSSLPISWNGLGVRENGYLLFLSPLFISR
                     EHALAFGVLWLSAVALSSIVGGLVLVFSKDLDSPAENGRVMKKWFDQRLTS"
BASE COUNT         1469 a         1710 c         1668 g         1452 t
ORIGIN      
        1 cgcccggcgg gtctcgtcga cagccaaatc gacatcgtgc agagtgatca acgccgatgg
       61 ccgtagcctt ttcggatcgg ctttgcagaa atcagccatc caattgttcc acgcgcggca
      121 aagatcattg gcatactccg cctccaggct gtcatcgcaa tggagcggga acgtgcgaaa
      181 cagaaccgcg acgtccaatc cttcttcgtc cattgcatcc agctgagata ttgcatcgta
      241 gtcgcgtttc aaagggtttc tgtaacgatg agccacttga gcttctcccg gagcgctttc
      301 tgccgcatcg gtctttggcg aagtggtggt caaaatctgc gtgccggatg gccggatcgg
      361 cttgccatcg gcataggcaa acttaatttg gccgtgtctt tttctcggct cgccccgtgg
      421 tatacgatcg ccccattttg ggtccatgta cttcaagtaa agatcgtgcg gctcgaatac
      481 atgcatgtcg ctatcgagga tcttgaatcc gtttcttgcc attgccttgt ctcctatccc
      541 ctagctttgg ttctgttgcc tactgataga ggctttccgt atagccgctg tcatcgagct
      601 ctttgacgaa gcgcaaatcg acgaaatcct ccggttttgc ttttttggat ctcggatcat
      661 tttctgccac atactctaat atagctttga tgccatccag agtgggatac tgttttctcg
      721 gcatgacggc ctcggaaaaa gagagatcgt aggtcttcac caagacctct cggtcttgaa
      781 gtcccttgaa atacttcgcg aggaccttca tccccgtttc cctgtcggtt ttgatacggt
      841 gcacggcttc gatgtaagac ttgacgaaac gccgaaccgt gtcggtattt tcccggataa
      901 acttacgcgt cgtgatcgct cctgtgtgcg gccattgaat gccgagagtc gaaatgtcga
      961 gaagaatgtt gagccctttc ttttgcgcca tcacgctggc gggaggatta agcggagtgg
     1021 cgacgactcg accggtttca agcgcgctga gccgtatggg gttattaccg accggcagca
     1081 aggtaacgtc ttttcccgca accaagccga atttacgtag cgcaaagcgg gcggcgaaat
     1141 cggtcgtcgt gccgaacgat ccgatggcga ttgcgccgcc tctcagttgt tccggtgact
     1201 tggtctcggg gcgagctacg agccagtagt tgatcgtagt cgttcccccc gcaatcatca
     1261 ccaggtccgc accgccaata ttgctgttaa tcaaaggcag acccgagcca tgggagatcg
     1321 ggcactcgcc ggagagaatc accgaaaggg atcgcggtcc cccgccgccg agaaagaccg
     1381 gctgaacatc cagaccgttt ttcttataga tccctgtctc atgtgcaaac cagatcggca
     1441 gcccctcggc gctgatcgca gtattgccga ccacaagttt ctgcgccgct ccaagtgatg
     1501 ggaatacgac aaccagaaaa aaagcccaag ccgctctgac taattgagac atggctgtga
     1561 cctcctggat tggttgtctg ctgattcgtg tcactttgtt caatcgccat tggcccgatg
     1621 ctgcctcgta atctgcggac cgttcatcgt gcgagtcgcg ctttacagtg tggctctaca
     1681 ctctcataaa cgcctttgtt ataggcttag taccggcaat cctaccctgt caatagaaaa
     1741 catttctctg ttatagcaaa gcgattatgt catgggccaa tggcaatttg attatgtccg
     1801 ggtcggttga gacccatcga gattctggta tccgatgctg tgctattgag tcggaagcac
     1861 ggtcggacca atttgccccg acgttccgcc tcctgtcttt gttgagatta gaataatttc
     1921 tccagacgca gcccgatcag ccggattgcc tttccgcgcg gattctcacg cgcatcgaag
     1981 aagggcagga gaagctcccg ggcttcgttt tgaatgcgtt gcagcgcatc gccgttgcta
     2041 tcgagcagga ttccattctt gatgctgcgg gagcgattcc gggtatcaaa atcggagaag
     2101 cgcaccgtca aggtcaacgt acgaaatcct gagaatccgt ttcttcgcag tttcgcaact
     2161 acccgttccg ccattttatc gagttgcgcg ctcaccaaag cgagatctcg gctatcgcgc
     2221 tcgaaggttt cctgttcgcc gacggatttg cgcgtccatt cgttggagac ctgagattca
     2281 tcgatgccgt gagccttttc aaaaagccgc agcccccaac tgccgaacca gtccgcgagc
     2341 gtcgcttgtg gaatttgtcg cagttctttc accaagcgaa cgctttgttg ctgcagaaag
     2401 gcttcgctct tgggaccgat gccgggaatt acgcggatgg gaaggggatc caaaaactcc
     2461 tgaacttcgt gcggcattat cacggttaag ccatttggct tatctcgacc ggaagcgatc
     2521 tttccgatca gcttgttcgg cccgatcccg atagagcagc cgagcccttc ttgttcgaga
     2581 atttcatttt taagcgcgat catattgacg cgggcaaggt caaagctctt taacgacgag
     2641 acgtccagat aagcctcatc gatactggcc tcctcaatcg catctgaaaa ccgttcgaag
     2701 atccgcatga ttcggccgga cactatttca taaagctgaa agttggaacg aacgaagatc
     2761 gtctcaggtt cgcccgccct gcatgctgcc tgagcaagcc gccaggcgcg cgagatcggc
     2821 agcgcggagc gaatcccgaa atcccgtgct cgataattgg cggtcgtcac cacacctcgg
     2881 ccccgtccat ccttgggatc ggcaccgacg accaccggaa gtccacgcag cgccgggtta
     2941 cagcgctcct cgatcgccgc gtagaatgcg tccatatcga catgaacgat gatgcgcatt
     3001 aaattactag agagctgtct cttatagtga ctcttacatg tttaacggct tcggataaat
     3061 catcgaccta cgactcgtct gaactcatga tcgaaacatg gcccgaaaag aaagcgccgg
     3121 catagtctta tatcgatatt gcgactctaa gttggagatt tttcttgtcc atccgggtgg
     3181 cccgttctgg aagaacaagg atgctggagc gtggtcgatt cccaagggcg agttcgagcg
     3241 cggcgaagac ccgctggaag cagccaagcg cgagtttcac gaagaaacgg gatattcgct
     3301 ggatggccgt ttcatccctc tcgagccggt gcggcaaccg ggcggtaagg tggtacatgc
     3361 atgggcgctc gaagggaatt gtgaagcgga atccatcaag agtaactcat ttatgatgga
     3421 gtggcccccg cggtccgggc ggcgccagga gtttttggaa gtcgaccgcg ccggatggtt
     3481 cagccttgaa ccggcacggc aaaagatact gaagggtcaa ctcatgctgc tcgacgaact
     3541 ccaacgaata ttgcaaagtc cttaggactt gctggagaag gctcccctgt ctaggtccaa
     3601 tttccgtccc agggaatatg agcgtgcgct gcgggaataa tcagcgccca cgcaatctca
     3661 taaccgcatc ccccgcatag gccatgaatt ggccggaagg tccggcccgg tttggactct
     3721 gcaatgaaaa cgccccagtc attgttacac cgtgggcatc ggcgagcgag gtatatggcc
     3781 atatctcaga ttaactctac cggtcaggct ccacaacgag gcccaaccgc actctcagtt
     3841 ctaatgagat acgcaactct gagctcgcag ctaacctcga aacaaccgga ctagcgcgag
     3901 tagagaaatt tgaactggcg gacgcggcgc tgtacggcag gggcatagat tgagtcggta
     3961 tgcctgtctt cgcagcgaaa tattatgctg caggtaggca gtggcggagt tgcggtcggt
     4021 cttgagtgga caaaaatgtg agccagtgtt caaaacctga caatattcct cgtgtgtttg
     4081 ggctcagacg caagcaaatt cgcgactatt tgaagcccca gtttggcatt tccattgctt
     4141 ttctcggtat gcactcagcg gaggttatcg aatgaagctg tttttggtac tgcggcaaga
     4201 aaccgctggg cagtgctcga ttgtagccgc tcgaagcaag cgagacgcaa tcaacaaaat
     4261 aacccggcta gagggtgtgg atgatgcctg ggcgattcgc atcgaagaat tcatgatcac
     4321 tttcaaaatg gaagagaata tcgcgttctt tcaacccgac ttagatcgcg cggttatgag
     4381 tatgaacatc gccaatcaac ggggtagccg cggaagaatc ttcaggttga aaggcgatgc
     4441 cgttatgaag aaaccgaaga tcgccaacga gactgcgata gcaattcatt agcggttgcg
     4501 gaggaagatc gttgcttggt gggtcccatt agtcgatcgc cgtgtcgctt gcgctcatcg
     4561 atttagttac gcgccgaacc cccggggttc caccgagcaa accatctagt ctttaaacca
     4621 gaatgatagg ggttcagctt ggcggcatgg gtaaagaaca atctcctgcg gcaatgttca
     4681 gatatctaaa aaaacgtaaa tgcccgagat gctattatga ttttgtcgtt gtgttgattg
     4741 attgcagtgg ttggatgcgg cgcatcaatg gcttttgcgg taaatgcgat cactatgtcg
     4801 actggcagtt atttaggtcg gaagttgttc aacgctcaag tgatgccgcg atgaatgccc
     4861 tatcgcgaaa tgaattgcac cgccctgctc cacgtttgat gatcactaga aaggcggccg
     4921 taaatctcaa acacgactcg tagaggtgaa tgctatgaac atagcccgcc tgcgaaaagc
     4981 ggatgagaca agctatcttt tcaaaccggt gaccggaagg tcaccggttt ggcaaatccc
     5041 gaaacgtcac gggcctgaaa acaccttttt tcaccggctg gctccagcct tcaccgctcc
     5101 gccatacacc aaggatcacg aggagaagct ccccacacaa gatctgttct gggtcgatat
     5161 gatcgtgaat gccttaattg tcatcgtgat agcttcggca agttattttt ttatctctgc
     5221 taaatttctt caaactatcg acccggcgat cagtccgtac tccgagcgat caccgtcgcg
     5281 ctaacttgca tctggctcca ggcacttgcc gggcaagcac cggactagta cggattccgc
     5341 attcatagcc gtccaaagtt gtcagttcct ccggacaccg gacttacagg accattccaa
     5401 gcgtttcgcc caacccgcat cgacgcgcgg gtctccgatt ggcgttcttg gcttgccggc
     5461 cgcgtgcgac tctggccagg tcttgcatca actggttaac ctctgatcga accatttctt
     5521 catcacccgt ccattctccg ctggcgaatc aagatccttg gaaaagacca acaccaaacc
     5581 gccgacgatg cttgaaagtg ccacggccga cagccacaac acaccgaacg cgagggcgtg
     5641 ttcacgagaa ataaacagcg gcgacaaaaa caacaggtaa ccgttttcac gcacacctaa
     5701 accattccaa ctaatcggca aagagctcag gatattgatc accggaatag tgactaataa
     5761 aacgggccag gggatctgaa tcccgaaacc ggccgccatg acttggtgca ggaatatctg
     5821 cagcaggtga aataccagcg aaatcaggga aatataactg atggtcgcga gatcgcgcgg
     5881 gaaaacctgg gcgatctttt ccacgttctt gtgcagcgcg ccttgttttg gcagcacggc
     5941 aagagctaga gcgggaccga aataccaggc cagcacgacg gcaccaatgc aagctgcgag
     6001 cagcgcaaga aattcaccgc cgatctgttg gcgaccgaaa agcgccatcg acacgatgcc
     6061 gatcgcggcc agaacggtca tcccatgcag acggtcggcg aacacggacg ccagggcggt
     6121 ggttttctgt tttgacttgc ttccgagcac gagagcgcgc gccaaatcgc cgccaaccgt
     6181 gccaagccca aagcaattca ccgccatgcc gaggaagtag gccctggcgg ccagcagcca
     6241 gggcgcttcg atgcttccgg agcgggccag gagccaccac ttgtaggaac ttagtagct
//
LOCUS       DASUVS010000367         3452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_811158, whole genome shotgun sequence.
ACCESSION   DASUVS010000367 DASUVS010000000
VERSION     DASUVS010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811158"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..2562)
                     /locus_tag="VF182_19605"
     CDS             complement(292..2562)
                     /locus_tag="VF182_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6439333.1"
                     /translation="MKSIEITRRQFLKTTGALVVSFNLLSPSRQALAQFATLPSGDID
                     PTSLDSWLAITPDGLVTFYTSKVELGTGTITALAQIVAEELDVPFEKIKMDSGDTSRT
                     IEQGSTVGSRTIERAGPQIRQAAAAARQELLKLAATQLNAPIEKLTVANGVVSVAGQG
                     SKKISYGELIGGKKFNTRITATGTGWDMKVAPEVKAKNPKDYKVVGKTIQRTDLPTKV
                     TGEHDYIHNVRLPGMLHGRVVRPPVINTEPLKIDQDSIRGIPDVVMIVREGKFVGVVA
                     KTEWAAIKAAQALKVTWTTPTSKVPANPDDMYKYLRSTKPVRSQKAVDKGDVAGSLSK
                     SKKIYQASYRWPFQMHGMIGPSCSIADVKSDKATIWSGPQGPFRTRGTIAALLKIPEQ
                     NIRVIYHEASGSYGRMSTDDGAEDAALLSRAAGAPVRVQWSRHDEHGWEPKGPAQLDE
                     AKAAVESDGRLLAWDFTDYSQPWTASGSTPLLASRQLGIMPKNPGGSNGTQSGGEIYT
                     IANQKIVAELINWHFPEPIPLRTSNLRAPGDVARCFASEGFLDEIAADLKLDPLEFRL
                     KNLTGNQRAIECLQAAAEKANWQKRPSPAAAASGNIAHGRGVALTQRARTYVAAVAEV
                     EVNKTTGEVAVKRVVCSHDCGLMINPDGVKNQVEGNIIQGVSRALYEEVTYDAKGVTS
                     LDWGTYPILRFPDVPELDIVLINRPEMQPLGAGEGATIPPAAAIANAIFDAVGVRLRE
                     GPFTPKRVLAAMQKKA"
     gene            complement(2578..3042)
                     /locus_tag="VF182_19610"
     CDS             complement(2578..3042)
                     /locus_tag="VF182_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020290809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6439334.1"
                     /translation="MSKISLRVNGKARMVDTESDVPLLYVLRNDLELNGPKFGCGMAQ
                     CGACTVIMDGNAIRSCVTPVGAVQDKPVTTLEGLGTTKKLHKIQQAFVDEQAAQCGYC
                     INGMIMTTKALLDKNPKPSDDQIKQALAGNLCRCGTHIRIVKAVKRAAGQTV"
BASE COUNT          664 a          955 c          987 g          846 t
ORIGIN      
        1 agcgccctga agagttgcct ttggacttaa ccgaggaact acatctcaaa ggaccggatg
       61 ctggaactct taagtatcga cggatgctaa gggacttact gtagagagac gtttatcggc
      121 aactcggtag ctgtggcagt ccaaataaga agctaacttt agatgaggaa ttctgcgccg
      181 gtcaaagcta tggcactttt gtacaggcat atttcaggtg cggtggctat gaaccgatcc
      241 tcatcggaca ttgagcttca ggagattaaa ttgcctctcc ctgcggcacg atcatgcttt
      301 cttctgcatc gccgccagca cgcgcttggg agtgaacggt ccttctctta atcgtacgcc
      361 gacggcgtcg aagatcgcgt tggcgattgc ggctgccggt ggaattgtcg cgccttcgcc
      421 ggcgccgaga ggttgcatct ccggacgatt gatcagaacg atgtcgagct cgggcacatc
      481 gggaaagcgc aggatcggat acgtccccca atcgaggctg gtcacgccct tggcgtcgta
      541 ggtgacctct tcatagagcg cccggctgac tccttgaatg atgttgcctt cgacctggtt
      601 cttcacgcca tcggggttga tcatcaaacc gcagtcgtgc gaacagacca cgcgcttgac
      661 ggcaacttca cccgtagtct tgtttacttc cacctcggcg actgccgcca cgtaggttct
      721 ggcgcgctgc gtcagcgcaa caccgcggcc gtgtgcgatg ttgccgctcg ccgccgccgc
      781 tggggacggc cgcttttgcc aattcgcctt ctcggcggcg gcttgcaggc attcgatggc
      841 gcgttgatta ccggttaaat tcttgagccg gaattccagc ggatcgagtt tcaaatcggc
      901 ggcgatttcg tcgagaaaac cttcgcttgc gaaacagcgc gccacatcgc ctggcgcgcg
      961 caggttgctg gtgcgcaggg gtatcggttc cgggaaatgc cagttgatca attcggcgac
     1021 gatcttctga ttcgcgattg tatagatttc accgccgctt tgagtgccgt tagatcctcc
     1081 gggattcttc ggcatgatcc cgagctgcct cgaagccagt agcggcgtgg acccggatgc
     1141 ggtccaaggt tggctatagt cggtaaaatc ccaagccaaa agcctgccgt cactctccac
     1201 ggcagctttg gcttcgtcca attgcgctgg gcccttgggc tcccaaccgt gttcgtcgtg
     1261 ccgtgaccac tgcacgcgca caggcgcgcc tgccgcccgt gacaataaag cggcgtcttc
     1321 ggcgccgtca tcggtgctca tgcggccata agagccggaa gcctcgtgat aaatgacacg
     1381 gatgttttgc tccgggatct tcaacaatgc agcgatggtg ccgcgggttc ggaaaggtcc
     1441 ttgcggaccc gaccagatgg ttgccttgtc gcttttgacg tcggcgatcg agcacgacgg
     1501 gccgatcatg ccgtgcattt ggaagggcca acgatagctg gcctggtata tctttttgct
     1561 tttactcaag cttcccgcca cgtctccttt atccaccgcc ttttggctgc gcaccggttt
     1621 ggtgcttctc aagtatttgt acatgtcgtc ggggttcgcc ggtacttttg aggttggtgt
     1681 tgtccatgtg accttcagtg cttgcgcggc tttgattgcc gcccattccg tcttggcaac
     1741 aacgccgacg aattttccct cgcgcacgat catgactaca tcgggaattc ctctgatcga
     1801 gtcctggtcg atcttgagcg gttcggtgtt gatcaccggc gggcgcacga cccggccgtg
     1861 cagcatgccg ggaaggcgca cgttgtgaat gtaatcgtgt tctcccgtta ctttggtcgg
     1921 tagatcagtg cgttggatcg tcttgccgac gactttgtaa tccttggggt tttttgcttt
     1981 gacctccggc gcaaccttca tatcccagcc ggtgccggtc gcggtgatcc tggtattgaa
     2041 ttttttgccg ccgatgagct cgccgtatga aattttcttc gagccctgcc cggcaacgct
     2101 cacaactcca ttggcaaccg tcagtttctc tataggcgcg ttcaactggg tcgcagctag
     2161 cttcaacaac tcctgtcgcg ccgcagccgc ggcttgccgg atttgcggcc cggcccgctc
     2221 gatggtgcgg ctgcccacgg ttgatccctg ttcaatcgtg cgcgaggtat cacccgaatc
     2281 cattttaatt ttctcgaacg gcacatctaa ctcttccgcg acaatctgcg ccagggcggt
     2341 gatcgtgccg gtgccgagct ctactttgct ggtgtaaaaa gtaaccagtc cgtccggcgt
     2401 gatggcgagc caggaatcga gtgaagtcgg atcgatgtcg ccggatggaa gcgtggcaaa
     2461 ctgggcgagg gcttggcgag acggcgacag taaattgaag ctgacgacca gagcgccggt
     2521 ggtctttaga aactgcctgc gagtgatctc gatggatttc atgtttgtcg ctcctcgtta
     2581 aacggtctgc ccggccgccc gtttgactgc tttgacaatg cggatatgag tgccgcaccg
     2641 gcaaagattg cctgctagag cctgcttgat ctgatcgtcg ctgggcttgg gatttttatc
     2701 gagcaaagcc ttggtcgtca tgatcatgcc gttgatgcag tagccgcatt gtgccgcttg
     2761 ctcatcgacg aaggcttgct gaatcttgtg cagcttcttg gtcgtgccca aaccttctaa
     2821 cgtagtaacc ggcttatctt gtacggcgcc gaccggtgtg acacaggagc gaatcgcgtt
     2881 gccgtccata atcaccgtgc aagctccgca ctgcgccatg ccgcaaccga acttcggacc
     2941 gttgagttcc aagtcattgc gcaggacata gagcagggga acatccgatt cggtatcgac
     3001 cattcgtgct ttgccgttta ctcgaagtga gatcttggac atggttggct cctttttgaa
     3061 ttgggagtcg gcgtgtgatc ttggctctat ttacgaaaac gccccgcgca aagtcaagat
     3121 tcgcagggtg atggtgccga gaacggagaa ccgcccagtt caactgctgc ggcagggtgg
     3181 ttcatgacac cgcgtattgc agtagtacca accgcgcgga gacccggtga atggaggtga
     3241 gtcacggatc accaaacttg atggagctta aagatttcac cagacagagc tcgaatttac
     3301 ggccggcatg acagacccat ccgtagtttt accggcatgt ccattaaaga agagaaaatg
     3361 atctcagttg agattgccgg ttttcggctc ttctctaaca aacgttagaa gcgccatgcc
     3421 ggcgatatag gtcccggctg cgatataaaa tg
//
LOCUS       DASUVS010000368         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_374287, whole genome shotgun sequence.
ACCESSION   DASUVS010000368 DASUVS010000000
VERSION     DASUVS010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374287"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            554..1804
                     /locus_tag="VF182_19615"
     CDS             554..1804
                     /locus_tag="VF182_19615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439335.1"
                     /translation="MPYPFSELRRVAGLSLAVALFLVPAVCLAQSSELEALKEMVKGM
                     QQDLQKALNRIEQLEKQKAADSAKLGQVETSIKTVQSTPSVLNPAIGLAIDATAEHRD
                     KSGGDFNLRAAELGISASIDPYARAYAFINANREEVELEEAAIVTTSLPWNLQARAGR
                     LFADFGRLAKFHQHEYPFVNAPLSLERMVGGESRADGVELSYLVPTPFFLRATLGGYN
                     KMGAENERLDDTRPRAWSRFTYLGRLQTYLDLADNHSVELGTSLAYTPSIRIPEDPQD
                     GSRSLAGLDLTYRYQPLGSTLYQGFTLGGELFGNSERVERASGFQRLQALGGYAYAEA
                     KLSREWGAGFLFDNTPSLSSPGKKTISYSPFLTWNLSEFNRLRFQYSFFDDSVREEKS
                     ERGNQFFLQWTTFLGSHTHGFRTR"
     gene            1837..>2313
                     /locus_tag="VF182_19620"
     CDS             1837..>2313
                     /locus_tag="VF182_19620"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX6439336.1"
                     /translation="MKAERRRILKLILVIGILVIFYSPASAQRIRVITTIPDLADMTK
                     QIGKELVEVESLTRGVEFMHAVPVKPSFVPKLNRTDVLVLMGLDLESSWLPALLEVAG
                     NAKILPGRTGHIDCSAGVNVMEVPKTLDRSEGDIHPRGNPHYNLDPLNGTIIARNIA"
BASE COUNT          526 a          626 c          613 g          548 t
ORIGIN      
        1 aggtatctta agccctcaag cccggctgca tgggggcata ctgaagatga cgcgccgaac
       61 atgcagagtc atagacctat ggcgcgcgcc ctggccggtt ctttatagcc ccaattagct
      121 aagattcatt gactttgctg aagccttagg tctaagttcg tgacgtgctc cagtcttcag
      181 cgccaaccgc gggtagtctt cgacacatca tcagtgttgc tgcgttgtta tttgtttttt
      241 ttctacctct tcattctcat ttctccggaa gcccgctgat cagcaaagaa tgtagctgca
      301 ttcaaggcac tcggacacat ttagcggtcg cggctgacac ttggaatctc gcaccgacat
      361 accgggtaac ttacgttatc gctcaggatg acgtgctttg gttaaacgaa cggcctaagc
      421 tccagcgcgt tcgtgcgccg cctacttctc tctccgttta gtttgaacag caaaatccta
      481 cagtatcggt cttcattgcc cctccgactt tcagtgggag ggctaacact ccatctcgga
      541 ggggaggctc gttatgcctt atccatttag cgaactgcgc cgggttgcgg gattatccct
      601 cgcggtggcg ttgtttcttg ttcccgccgt atgccttgct cagagctcag agttggaagc
      661 tttgaaagaa atggtcaagg ggatgcaaca ggatttgcag aaagcgctta accgcattga
      721 gcaacttgaa aaacaaaaag ctgccgattc ggcaaagttg ggtcaagtcg aaacatcgat
      781 caagacggtg caaagcacgc catcggttct caacccggca atcggcttgg cgatcgacgc
      841 caccgcggaa caccgcgaca aatccggtgg agatttcaac cttcgcgctg ccgaactggg
      901 gatttcggca tcgatcgatc cgtatgcgcg agcttacgcc tttatcaatg caaacaggga
      961 agaggtcgag ctggaggagg cggcgatcgt taccacttcg ctgccctgga acctgcaagc
     1021 cagggcgggg cggttatttg cggacttcgg gcgtttagca aaatttcatc agcacgaata
     1081 tccgttcgtc aatgcgcctt tgtcgcttga gcgaatggta ggcggagaat ccagagccga
     1141 cggcgttgaa ctgagctatc tcgttcccac gccatttttt ctgcgggcaa ccttgggcgg
     1201 atacaacaag atgggcgcgg aaaacgagcg tctggatgac acgaggccgc gcgcctggag
     1261 ccgctttact tatttaggca ggctgcagac ctatctcgat cttgctgata atcacagtgt
     1321 cgagctcggc accagtctgg cctacacgcc ttcgatccgt attccggaag atcctcagga
     1381 cggcagccgg tcactggccg gtctcgattt aacgtaccgc tatcaaccct tgggcagcac
     1441 tctttatcaa ggatttactc tcggtggaga actttttggc aacagcgagc gggtcgaacg
     1501 cgcatccggt ttccaacgcc tgcaagctct tggcgggtat gcctacgccg aagccaaact
     1561 gagtcgtgag tggggcgccg gatttctttt cgataacact ccaagcctga gtagccccgg
     1621 caaaaaaacc atcagctatt cacctttcct gacctggaat ctgagcgagt tcaatcgcct
     1681 gcggtttcaa tacagctttt tcgacgatag cgtccgagaa gaaaagagcg agcgcggcaa
     1741 tcaattcttt ttacagtgga ccacattcct gggcagccat acccatggtt ttcgcacacg
     1801 ctgatccagc tgttgttcac agaggaggga ttactcatga aagcagagcg cagaagaatc
     1861 ttaaaactaa ttctggtcat cggcattctg gtcattttct atagcccggc tagcgcgcaa
     1921 cgaatccggg tgatcacaac cattcccgac ctcgccgata tgaccaagca gattggcaag
     1981 gaactggtcg aagtcgaaag ccttaccagg ggtgtggaat ttatgcatgc ggtgccggta
     2041 aagccgagtt ttgttccgaa actaaatcgc accgacgtgc tcgtgctcat gggactggat
     2101 ctggaaagtt cctggctgcc ggctctgctt gaagttgccg gcaatgcgaa gattctgccg
     2161 ggccgaaccg gtcacatcga ctgttctgcc ggcgttaatg tgatggaagt gccaaagact
     2221 ctagaccgct cggaaggcga tattcacccg cgtggcaatc cgcactacaa tctcgatccg
     2281 cttaacggca cgatcatcgc gcgcaatatc gcc
//
LOCUS       DASUVS010000369        52001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_812906, whole genome shotgun sequence.
ACCESSION   DASUVS010000369 DASUVS010000000
VERSION     DASUVS010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 52001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52001
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812906"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VF182_19625"
     CDS             <1..342
                     /locus_tag="VF182_19625"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HEX6439337.1"
                     /translation="KTMEGVASFAGTILKSGFERNWLSRLGYLISRESLRHAYRKLDY
                     AEYGGARLLGLDGVAIVAHGGSTALAIKNAIRVASESVSQDVNGHISAALESIVQAET
                     ESKVLKTTIGP"
     gene            385..1329
                     /gene="fabD"
                     /locus_tag="VF182_19630"
     CDS             385..1329
                     /gene="fabD"
                     /locus_tag="VF182_19630"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX6439338.1"
                     /translation="MASTNSVAFLFPGQGSQYVGMGKELCEHSSVAAQAFQEAEQTLG
                     FSLKQLCFYGPEADLQLTENTQPAILAVSVAALRVLQAEIAVQPAYVAGHSLGEYSAL
                     VCVGALSFSDALKIVRERGRLMQAAVPSGKGAMAVIMGLDVAAVSSICKQAEDGEVVA
                     PANYNGGGQIVIAGSKAAVARAMLLAKSEGAKRVVPLPVSAPFHCPLMKPAAEGLKRI
                     LNDVTIHPFTVGVIGNVNADVNLDPAQIKLLLAEQAMKPVRWEESVQRLENLGVSKML
                     EVGPGRVLKGLVKRISPKIAVDTFEVPQDLARLRNDIR"
     gene            1326..2069
                     /gene="fabG"
                     /locus_tag="VF182_19635"
     CDS             1326..2069
                     /gene="fabG"
                     /locus_tag="VF182_19635"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX6439339.1"
                     /translation="MKSNGQVALVTGASRGIGRAIAVALAQAGAYVVVNYRGNQEAAE
                     TTLEEVRVVGGQGELCQFDVAVETEIDAAVKKIVDGQGKIDILVNNAGVTSDTLLIRT
                     KAQDWDQVVDTNLKGTVLCTKSVCRLMIRQRYGRIVNLSSVVGQTGNAGQSIYAATKA
                     GILGFTKSIARELASRGITVNAVAPGFIETEMTATLSPQLQEEFLRSIPLGRFGKSEE
                     VAQLVVFLTGSGASYITGQVMNVNGGLYM"
     gene            2102..2338
                     /gene="acpP"
                     /locus_tag="VF182_19640"
     CDS             2102..2338
                     /gene="acpP"
                     /locus_tag="VF182_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX6439340.1"
                     /translation="MASSVQARVKEIVCEQLGVSEDEVVPEASFIEDLGADSLDIVEL
                     VMALEEEYEMEISDEDAEKIKTVQDVISYIDNHR"
     gene            2481..2969
                     /gene="rpiB"
                     /locus_tag="VF182_19645"
     CDS             2481..2969
                     /gene="rpiB"
                     /locus_tag="VF182_19645"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX6439341.1"
                     /translation="MIVMASDHGGVELKDFLAEVLRSKDIDLQDLGTYDRKPVDYPDF
                     GHRVSERVSQGDANRGILICTTGIGMSILANKYPNVRAALVQDRETARSSREHNDANI
                     LVLAGATTDPALAREILEIWINTPFSGGRHQRRLDKITEIERALGTLRKAPASKSDKD
                     SE"
     gene            2969..4222
                     /gene="glyA"
                     /locus_tag="VF182_19650"
     CDS             2969..4222
                     /gene="glyA"
                     /locus_tag="VF182_19650"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX6439342.1"
                     /translation="MSFLQQTDPEVFAAIQRETNRLEHNLELIASENVVSEAVLEAQG
                     SVMTNKYAEGYPGKRYYGGCEFVDEVESLAIARARELFGADHANVQPHSGSQANMTVY
                     LSVLKPGDCYLGMNLAHGGHLSMGSPVNFSGILYRVVPYGVSKETEIIDYDEVAKLAR
                     EHRPKLIIAGASAYPRIIDFAKFRQIADEVGALLMVDMAHIAGLVAAGLHPSPVPHAH
                     FVTTTTHKTLRGPRGGLVLCRAEYAKTVDSKVFPGMQGGPLMHVIAAKAVALKEALNP
                     AFKIYQHQIVKNAQALAKSLSSNGYRLTSGGTENHLMLVDLRKSELTGKVAQETLDRA
                     HITVNRNAVPFDTRSPFITSGIRLGTPAVTSRGMKEEEMGLIGNLIARALDRAGDENV
                     RSEVANEVAALCRKFPVYPHRLPTN"
     gene            4341..4844
                     /gene="nrdR"
                     /locus_tag="VF182_19655"
     CDS             4341..4844
                     /gene="nrdR"
                     /locus_tag="VF182_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX6439343.1"
                     /translation="MKCPFCHETENRVIDSRLSKDNNMIRRRRECSKCSRRFTTYERV
                     EEMMPLVVKKDGRRETYERIKIINGLKRACEKRPVSINTIEAIADRIERNLQERGEKE
                     ILSSVIGEATMRELHDTDEVAYVRFASVYRSFKDINEFMVELEELLKERKEMSAARVS
                     APRQKRN"
     gene            4848..5978
                     /gene="ribD"
                     /locus_tag="VF182_19660"
     CDS             4848..5978
                     /gene="ribD"
                     /locus_tag="VF182_19660"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX6439344.1"
                     /translation="MVLPREELDRRYIRTACRLAEKAAGRTSPNPMVGAVLVRQGKMI
                     ASGFHQFAGGDHAEIVALKKAGSKAKGATLYINLEPCNHYGRTPPCTRSLIAAGVKEV
                     VVGMKDPNPLVSGKGLRAMARAGIKVRSGVLQEECRVLNEAFTKFITRGVPFVTLKLA
                     ASLDGKIATPNGDSYWISCEESRAIVHQLRNSFDAVLVGAGTVITDDPQLTCRIAGGR
                     DPRRIILDARLRIPLDAEFLRQRGSDKNIIVTSHTAPAGKIRALENLGATVWRLPVRE
                     GAVRWLPLLRKLAKSGIVSVLLEGGAITAAGALKEKMVDKVLLFYAPKLLGGDARSMI
                     ASLGVERIDQSIMLQNLQIQKSGTDLLVSGYFNTSAAHDRVR"
     gene            6000..6605
                     /locus_tag="VF182_19665"
     CDS             6000..6605
                     /locus_tag="VF182_19665"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX6439345.1"
                     /translation="MFTGLIEDVGRVASSVLNKQSAVLTVETGLALREIPLGASIAVN
                     GACLTVVRKGKRSVAFDVSPETMERTSLATVEPGTLLNLERSMRLKDRLGGHLVTGHI
                     DGVGIVRSVQRKGDFTFVSFRIAQSLGSLLVSKGSVAVDGISLTVNRCNAREFSVAII
                     PFTFQNTNLRARRVGDKVNIEADLIGKYVHHFVTQRTQNPL"
     gene            6635..7861
                     /gene="ribA"
                     /locus_tag="VF182_19670"
     CDS             6635..7861
                     /gene="ribA"
                     /locus_tag="VF182_19670"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX6439346.1"
                     /translation="MPVSSIDEALEDLRQGRMTILVNEEAPDSDGFFCIPAEKVTAEI
                     INQMLHYGRGVIYLALTDERIRQLGILLIPHESSNFSGHLFGAAFSARLEGVPAVSAK
                     GRAHTIQVAVADQAKHADLIVPGHIQPIQARSGGVLARSGRAEASIDLARLCGFTPAG
                     VICHILKDDGSVALLPDLEKLAERLEIRVVAVTRLIAYRLRNESLVKRAAEEIFPTIH
                     GGMYKAIVYRNEVDASEHMALVKGDISNPDSVLVRLHSECLTGDVFGSERCDCGDQIR
                     QSLKLIDQAGKGVLVYMHQEGRGIGLTNKIKAYALQDRGFDTVEANLELGFKEDLRDY
                     GIAAQMLRDMGVKSVRLLTNNPRKISGIEEYGVTVKERVPLEVLPRDSNIHYLRTKQK
                     KLGHLLTNLEAKPGKS"
     gene            7871..8341
                     /gene="ribE"
                     /locus_tag="VF182_19675"
     CDS             7871..8341
                     /gene="ribE"
                     /locus_tag="VF182_19675"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX6439347.1"
                     /translation="MANVIEGSIDARGMKIGVIVSRFNKFITAKLLEGALEGLKTHGG
                     ADDDLTVVHVPGAFEIPIIAAQMAASGKFDAVVCLGAVIRGDTPHFEYVCDAATHGIS
                     KAVWEYRIPISFGVLTTDNVQQAMERAGVKDTNKGYEAALSAIEMVSVLRQLAR"
     gene            8409..8825
                     /gene="nusB"
                     /locus_tag="VF182_19680"
     CDS             8409..8825
                     /gene="nusB"
                     /locus_tag="VF182_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX6439348.1"
                     /translation="MGIRRKGRELALQALYQIEITGDASAAAVDLFLSHFEGSAQAKE
                     FARRLVSGVVSQRPNIDRVIDHCTENWKLARMAKVDLLILRISAYELIFCPDIPLNVS
                     LDEAIEMGKRFGSGDSGSFINGVLDQLAHAEIAKAH"
     gene            8830..11424
                     /gene="leuS"
                     /locus_tag="VF182_19685"
     CDS             8830..11424
                     /gene="leuS"
                     /locus_tag="VF182_19685"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX6439349.1"
                     /translation="MEPRYQAKEIEEKWQTFWEEHQSFQASEAPGKPKYYVLEMFPYP
                     SGRIHMGHVRNYSIGDVVARYKRMRGYNVLHPMGWDAFGLPAENAAIERGIHPEKWTQ
                     ENILYMKAQLKRMGLSYDWSREIATCRPEYYRWNQWIFLQFYKRGLAYKKSSLVNWCP
                     SCETVLANEQVIEGVCWRCESTVTQKELDQWFFRITDYAEDLLHDLERLPGWPDKVLL
                     MQRNWIGKSVGAEINFPLAGRAEPLKVFTTRPDTVFGATFISLAVEHPLALKLAQGLP
                     QEDAVQEFIDTIKQQKSIGRGGEEGEKEGIFTGAYCTNPFTSENIPIYLANFVLMEYG
                     TGAVMAVPAHDQRDFEFARKYGLPVRVVIQPAGSVLRAQELTSAYVDEGVMVNSGVFS
                     GLTNIDGKEKIAEHLEKQGWGKKAIRYRLRDWGVSRQRYWGTPIPIIYCDACGVMPVP
                     EDQLPVTLPIDVPFTGKGRSPLAESKDFSEVQCPRCGAAARRETDTMDTFVDSSWYYL
                     RYTSPKSFTTPFDAKQAAYWMAVDQYIGGVEHAVLHLLYARFFTKALRDLGVLQVDEP
                     FTNLLTQGMVCKETYRCPEHNWLLPTELAGSEKDGWRCPQCARLVEKGRVEKMSKSKK
                     NIVDPEELVAAYGADTARLFTLFAAPPEKDLEWSEQGVEGAYRFLSRLWRFVFHNREM
                     LLGGETDSPELNGSSDVRELHRMIHRTIKKVTDDIDGRFHFNTAIAAIMELFNALSIA
                     AGRQEGQLAAPVIRTGMETIIRLLYPFVPHLMSELWLSLEKSQPLDQLSWPEYSPEAL
                     DEEQFLIVLQVNGKVRGKIKVPANTQRDQVESDALAEPRVKAFIGGKKVERIVYVPRR
                     LVNIVLEG"
     gene            11421..12050
                     /gene="lptE"
                     /locus_tag="VF182_19690"
     CDS             11421..12050
                     /gene="lptE"
                     /locus_tag="VF182_19690"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX6439350.1"
                     /translation="MKLRDTLRETIRRATALTILGYFCATLTMAGCGYSFAGRSETFP
                     DDIRTVYIEPFINQTRNVGLEKELTSALRSEFYRRGQLRVVDNIDQADGIVAGVVRTF
                     ESHVASVNRRDEVLQYETAIVIDASLRRREPNEIIWRSQGTRLTDIYGGSRAAVVTTS
                     SEFKTGTLNASDVRRLTDIQLTETENRAVRGQLVERFARELHQRLMEMF"
     gene            12427..14112
                     /gene="murJ"
                     /locus_tag="VF182_19695"
     CDS             12427..14112
                     /gene="murJ"
                     /locus_tag="VF182_19695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX6439351.1"
                     /translation="MNEEAGKLTRAAGVVGFFTLLSRVSGLFRDVVIGFLFGAQGAAD
                     AYFVAFRIPNLLRRLTAEGALTVAFIPVFTNYMAKEGRAEAIRVTQIVFTFLTIFLGL
                     ITLLGIVFARPLTQFFAPGFVQEPEKFYMAVSLTRWMFPYIFFVSLVALAMGVLNALR
                     HFMAPAMSPVLFNLCNIICAIVFYPLLDEPILGLAFGVLIGGAAQLLFQIPYLLKQGI
                     RLRFNFDFRHPGLGRLLYLMGPAAFGAAVYQINVLVSTMLASFLPSGSVSYLYYADRF
                     LEFPVGIFAIALGTAALPSFSSLVVKGNIQELRATLAYALRIVNFICLPATAGLAVLA
                     VPLFAIFFQRGAFDATATLNSAQALIFFSFGLWSISGTKLVAPVFYAMEDTKTPVLVG
                     IIAFMVNLLASLALMGEIVLEADSAGIMARTMAMASVRIGLSSLGHGGLALATTISSL
                     CNFFILLLILNSRLKGIPLREVAYSFFRNLITTLIMAIPLIWVVGNVDWTGTTLPIYS
                     KALILAGLIVLGLTLYLAFSYLLKSPEWPIVEQFRRALQSRLCRLTPKRRPEG"
     gene            complement(14561..14713)
                     /locus_tag="VF182_19700"
     CDS             complement(14561..14713)
                     /locus_tag="VF182_19700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439352.1"
                     /translation="MLEPLYRHPVSELLVPVPPEPALALQLVEAEAAVAPALLSCRTL
                     QLRESK"
     gene            14850..15578
                     /gene="tatC"
                     /locus_tag="VF182_19705"
     CDS             14850..15578
                     /gene="tatC"
                     /locus_tag="VF182_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016647029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX6439353.1"
                     /translation="MASDSYLTFSEHLEELRWCLLRALAAIVVASLVCYFFADAIFAF
                     MVAPLKQILPPGQNIIGTTVTEAFFTEIKVAFLAGVLFSSPFIFYEIWRFIRPGLIGN
                     EGRLVIPFVVCATLFFLGGAYFCYRIVLPVAFSYFIEQYGTLGVTPAIRLGEYFTFFF
                     RMVLAFGITFELPIITFVLVRLGVWDHRFMRRSFRYAVIVIFILAAFLTPTPDIINQL
                     LLAVPMLLLYILSIGVAYIWRKAE"
     gene            15614..17737
                     /locus_tag="VF182_19710"
     CDS             15614..17737
                     /locus_tag="VF182_19710"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX6439354.1"
                     /translation="MVAFVIAISFLSLGVAVFLARWVLARETGTPKMREISDAIKEGA
                     EAFLSRQNRTIISMAAILATVIFFLYAYVRAPSPADPVSPVEMAFWTTFSFVLGALCS
                     VIAGYIGMWVSIRANIRTASAVRTSLNEGLRIALRGGAVSGLFVVAMSLLGVGGLYAL
                     LEFLGHDPQKIPFTIVGYGFGASFVALFAQLGGGIYTKAADVGADLVGKIEAGIPEDD
                     PRNPAVIADLVGDNVGDCAGRGADLFESTAAENIGAMILGVGLYPYFGIKGIVFPLVA
                     RALGLLASIVGIMIVKTDENGDPMHALNRGYYVTSGLAMVGFFVCAQWLLAVDPTKYP
                     EAQSAWFYFFLCGVIGILMAQAFVYITQYYTEYKYRPVREIAEASQTGPATNIIAGIA
                     VGFECVAAPVLVISVAIIASYYLGLNSGVKNAGLFGTAVATMGMLGTAAYILAMDTFG
                     PITDNAGGIVEMSGQPEEVRRKTDRLDAVGNTTKALTKGYAIGSAALAAFLLFSAYLD
                     EVAHYGHRLEAVNIAKPEVFVGALLGAMLVFLFSAYAIRAVGKAAYYVINEVRRQFKE
                     NPGILQGTVKPDYRRCVDIVTVGALKQMILPGVVAVTMPIAVGLVFRFFGVGAEAVAA
                     FLMVGTIAGILVATFLNNSGGAWDNAKKYIETGAYGGKGSEPHKAAVVGDTLGDPFKD
                     TAGPSLHVLIKLLATITLVLAPLFV"
     gene            17831..17917
                     /locus_tag="VF182_19715"
     tRNA            17831..17917
                     /locus_tag="VF182_19715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17866..17868,aa:Leu,seq:taa)
     gene            18090..18326
                     /locus_tag="VF182_19720"
     CDS             18090..18326
                     /locus_tag="VF182_19720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439355.1"
                     /translation="MPQYEIHVLCNECGAEHPMRVRLHLDDGPAEKRSIGETYKGRTQ
                     PPQLLAVGGHKTLCLKTGKMFVQENEDQIFLIPR"
     gene            18537..18710
                     /locus_tag="VF182_19725"
                     /pseudo
     CDS             18537..18710
                     /locus_tag="VF182_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433871.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
     gene            19403..20347
                     /locus_tag="VF182_19730"
     CDS             19403..20347
                     /locus_tag="VF182_19730"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX6439356.1"
                     /translation="MKFESTKEVSVAFAKIGIMSIGEMGYHWAKLLTARGAEVLTYDR
                     DRSPVTQKRAENAGVRSTGSLSELCSSADLIVSIVVPTAAINVAAEIAKAATHAKRKG
                     LIYFDANAISPMTAETISGVLTESAVEFVDGCIIGSAAKMDKGSVVYASGPAATRIAV
                     LGDYGLSVRVLGTGTSQASAFKVVYAGLTKGLQGLFVELLMGAKRFGLLDEIVNRYDE
                     SFPGLLEKVSSSIVGLRNHAARRAEEMDELQRTYAHYGMTALMAPAVQKVLGSIADLA
                     IRESSEKGARAGDLMETLELFYQQGLLSDARLASSASK"
     gene            20404..22185
                     /locus_tag="VF182_19735"
     CDS             20404..22185
                     /locus_tag="VF182_19735"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6439357.1"
                     /translation="MAKSERRVEKKQPQYGSDVVVDLMKAHDIEYAAFNPGATFRGIH
                     DSIVNYGGNYKPEVIFCCHEEFSVAIAHGYAKAKGKPMIAIVHNMVGLQHASMAIFNA
                     WIDRVPIIVLGGTGPMNTKRRRPRIDWIHTALVQGNQVRDYVKWDDQPYTLADVPDSF
                     IRGYRIATTEPMAPVYINYDADIQEESVSAPVELPDISRYAPPAPVQANLAQLKRAAE
                     LLVNAQMPLIIADSLGRNPQTVPALVELAELLAIPVIDKGARFNFPTTHPLDATDGAK
                     DILSKADVILALDVADLYGSLTTVSKQTRDCVYVTSPSVKIITISMHDMLVRSWAGDY
                     QALQPTDIPLTADTSVAVPELTRLCRELLGNDSKRKSTMEARQKELADKSKSRRAKWM
                     ADAQAKASLQNISTAFLAYDLGEVIKKEDWVLVNGTSNGWARRLWDFTKPNQYLGASG
                     GAGVGYGASAAIGAALALKGSGKVAVDIQSDGDLLMTSSCLWTAAKHKIPLLIVLHNN
                     QSFYNSEEHGIEVAKFRNRPVENAGIGTHVDDPEVDYAKMAQSFGVHAEGPIRRTSEL
                     RPAIERALKYVKERQLPAFVDVVSEPR"
     gene            22196..23026
                     /locus_tag="VF182_19740"
     CDS             22196..23026
                     /locus_tag="VF182_19740"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6439358.1"
                     /translation="MSNLYLAFREAIDCAEDQIDLGRAALAIATQEYPDLKIDDCLSQ
                     LDQLGQAVELRMGDEKNPYRIIAALNTVLFKEQGFQGNHSEYYDPKNSFLNDVIIRKK
                     GIPISLSVIYMEVARRVGLTLEGVGFPGHFLVKYHEGDIEILIDVFNDGEIRAREDLD
                     QMLQQLYRGQVSYQPGFVATLGKRDILRRMLNNLKWIYLERGEPLKTLSVLDQLVILD
                     PSAASEIRDRGLLYITLECYAQALDDLESYLRLAPGAEDAPMIKAQIESVRHRYVQIQ
                     "
     gene            complement(23051..25267)
                     /locus_tag="VF182_19745"
     CDS             complement(23051..25267)
                     /locus_tag="VF182_19745"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6439359.1"
                     /translation="MIHQEKSFEKFRIVLIKPSKYDDDGYVIRFWKGVLPSNTLNVLH
                     GLTEDVKQRRVFGELPIEVTTFDETAEKVPLHKIIRWSSEPHTRLLVCMVGVQTNQFP
                     RALDMARVLRAEGITVIIGGFHTSGTVNMLGEQEPDMQELFREGICAVSGEVEGKWDM
                     ILADFLEGKLKPLYSFAQDLQSLVDIEDSPLPKTSAKTMEHFAYPSFGTADTSRGCPF
                     ACSFCTIINVQGRKMRERSPQSIAAMMRRNYLDHGVSFYFFTDDNFARKKLWRETFEE
                     IIKLREEGLDVTFMMQVDLARKPKDFVSLAAKAGCTQVFIGMESVNPQNLASEGKKQN
                     KVEEYREIIQEWHQVGVVVHTGYIIGLPWDSKQQVPNDIRFLIDEVGPDQASFFMLTP
                     LPGSHDHREMKKRGDWMDPDFNKRDSFHATIKHPLMTEEEWTECYEKAWRTFYSKENM
                     IKVLSRWTDNPRNYWNLMSIFLWYKNAALIEKQHPMVAGFFRLKDRKARRPGYAIDSI
                     TVHAWKRSKEISRLLVNWAKFLKEMEEVWLQTRKKSDTEERWVEQIQKIQTDIWQALR
                     IGELQKVYSSARETLSGRARALLDPLEELSSRILYNRSDLNRFLKQWEHLRNKIPEIR
                     SAETDAARSCLDEMHNLQANVRLTDRVNEWQEAYTRLRGSLPSRASLRLIKFDAINYR
                     VVYSRQELQRIWSHTIHNARQMKLWHIRPWRLTRAMIQDFSLTTSFAANFKEFARR"
     gene            25419..26102
                     /gene="ftsE"
                     /locus_tag="VF182_19750"
     CDS             25419..26102
                     /gene="ftsE"
                     /locus_tag="VF182_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX6439360.1"
                     /translation="MIQLLHVSKTYPPNSPALIDITVSIDKGQFVFLTGPSGAGKTTL
                     LKLLFREEEPSEGQIMIDHQNVTRLRTRGVARLRRRIGLVFQEFRLLPRLTVMENVAL
                     AAQVVGVSRREAQIKAVRLLRELGLEKPQARPLMLSAGEQQRVAIARALINEPRLILA
                     DEPTGNLDPALAEEIMRIFVKLREAGTTLIVASHNLDLIKRYGSRILSLRQGRLVDDL
                     ERVMEAHAS"
     gene            26099..27007
                     /gene="ftsX"
                     /locus_tag="VF182_19755"
     CDS             26099..27007
                     /gene="ftsX"
                     /locus_tag="VF182_19755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX6439361.1"
                     /translation="MSWSHFGFVARRVRHSLWELLWSHILTSGTMAMSLFVFGVFILV
                     QENLQHLLNGWGDQIQVNTYLENNAGTDQVEAIMVRIRAMPEVESLRHISKEQAWNDF
                     QTALGAQSAVLEGLPEDVLPESFEITLKPAYRDPPQIENFAGRLGKINGIAAVEYPQQ
                     WVDRLNLFVLALQWAKWGLGGILFIATFFIVGSTVRLALLARKDEIEIMQFVGASEEL
                     IRAPFVVEGMLQGTVGAGLSLLGLWLFYVLLEKYLVGQLALFEPKMRLLFIDPASITM
                     ILVIGLLLGALGSLFSLRRFLKTWRG"
     gene            26995..28143
                     /locus_tag="VF182_19760"
     CDS             26995..28143
                     /locus_tag="VF182_19760"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX6439362.1"
                     /translation="MARLAAMGVFLAAGLVAALAHAASARRDLEGIQKKIESEKRVLS
                     QVNKKEGSVIQSLSQVESELERKTKQLKQSTARLQVVVAEMQKKEAEALRMRESLEQR
                     KTYLARRAESLYRWQRSGSPLVIFNGDLSLSSLLKRKYYLQRTVAFDREMVQSLTDQA
                     AHQEMLAQELARKRQDVDNQRRDLAEVKEAVQKEAQKKRELLASVREEKESRQRALKD
                     LEQAAVRLQIMIEELSRRSAAQSKEAPAAGGLESMRGKLDWPVKGQVLGSFGKTKHHE
                     FATEVFRKGLEIEAPVGEEIRAVEKGKVVFADRFSGYGKMLIIDHGDRYYTVYAHLSD
                     FLKKNGDLVRRGEAVALVGDSDSLAGSKLYFEMRKDGKSIDPAPWFRR"
     gene            28215..29573
                     /locus_tag="VF182_19765"
     CDS             28215..29573
                     /locus_tag="VF182_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX6439363.1"
                     /translation="MRSMTKHGVSLSILLIGIALGFVLAGQWVPNVSAVPRQDYETLE
                     TFTNILSIVKKNYVEDVDTKNLVNGAINGMLTSLDPHSAYLTPDLYKELQMDTQGRFG
                     GLGIEITVKNGVLTVVSPIEDTPAFKAGVKPGDMIFKIEDDFTKDMTLIDAVKKMRGP
                     KGSKINLSIKREGIPELINLSLTRDTIRVQSVRSRTLEPGYGYIRLAQFQERSDRDLQ
                     RALEKMAAEKGGLKGLVLDLRNNPGGLLTQAVRISDIFLDSGLIVYTDGRIESQKQKF
                     FAQKDASSMDFPLVVLVNGGSASASEIVAGALQDHKRAVVLGTKTFGKGSVQTILPLD
                     DNSALRLTTARYFTPNGRSIQATGIVPDITVESTPQQDGKLDERKRPSLREENLPGHL
                     QNPQQNPSPQEQLDQEREKQIPSSAPTGDETIDNDAQLKRALDLLKSWDVFKQVVQKK
                     AA"
     gene            29644..30867
                     /gene="xseA"
                     /locus_tag="VF182_19770"
     CDS             29644..30867
                     /gene="xseA"
                     /locus_tag="VF182_19770"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX6439364.1"
                     /translation="MRKEADLPLFTSTPKYLTVSQLNQLVRVTLETRLDAVWVLGEIS
                     NFRVPPSGHCYFTLKDNNSQISAVLFRRQGQNLAFVPENGMQVLCFARVSLYTARGDL
                     QIYVESLEPRGQGALYLAFEQLKKKLAQEGLFAAERKRPLPFLPDTIGIVTSDTGAAL
                     HDMLRIIRDRYADRRVIVRPVKVQGSGSAKEIAAAISDLCTTGVQVMIVGRGGGSLED
                     LWAFNEEVVARAIFACSVPVISAVGHEVDFTIADFVADQRAPTPTAAAEMVVPFKQDL
                     LAQLQLIENQLLRCMQSEIDNGHEICRGLVKRLLDPGRKLREKQQHLDDLSLDLQRRF
                     RGRLNQFKEQLGHEGSRLNALSPLAVLDRGYSITHKMPDEVIVKSADSLDVGDMVRIT
                     LAQGKADCRVEGKER"
     gene            30864..31754
                     /locus_tag="VF182_19775"
     CDS             30864..31754
                     /locus_tag="VF182_19775"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6439365.1"
                     /translation="MSILVLLLCFLDSAIYAAEPFALSWPSRLEIPRGEIAELKVYGE
                     RLSSVEGQWGAENLPFYAVAPGSFVAMIGVDLEAKPGVSKITLTATNDAGRQRQAETI
                     VQTLSKPFRQESFTVAPGFDQLSPQALELIRIERADFALAFSTPAAQRLWEAPFIRPV
                     PQESSSSFGYRRIINGKPRAPHTGADLRAPIGTEVLAANHGRVVLVGNYFFAGQSIVL
                     DHGGGLYTMYFHLSDFKVDAGALVRRGDVIALSGMSGRVTGPHLHWAARLGNARVDPM
                     ELIQKISSGPSQQPGSATGS"
     gene            31772..32035
                     /locus_tag="VF182_19780"
     CDS             31772..32035
                     /locus_tag="VF182_19780"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX6439366.1"
                     /translation="MVKKESQELKFEEAIANLEQVVEQLESGDLSLEDSLAVFEKGVG
                     LVRYCNQRLSEVEKKVELLVKDKDGQLQLKPFGNPAADSESDQ"
     gene            complement(32082..32795)
                     /locus_tag="VF182_19785"
     CDS             complement(32082..32795)
                     /locus_tag="VF182_19785"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6439367.1"
                     /translation="MTSHSGSLAEIATAKSMRIIARAVAFGRLETISSGLDHIPATGP
                     AIIVVRHYHHFYDGLALFAALPRQFHIVVALDWTQNAAIHSVMTQLTRLARWPVVLRA
                     DALQHKNDRGRSPSFSLDDTQRYQRRAFRESVQLLVDHRLLVIFPEGYPNIDPSYTPK
                     MDKHGFLPFKRGFFAIACAAERRLGISLPIIPTGLHYSSGKTWKAHVRFSEPLKRESF
                     ASDNELIRCCEEKVRQLSA"
     gene            complement(32817..33914)
                     /locus_tag="VF182_19790"
     CDS             complement(32817..33914)
                     /locus_tag="VF182_19790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6439368.1"
                     /translation="MAKTSQGRLVETSDQPSSARITILLPVLNEATRIANCLESLIAQ
                     PNEVAEILVIDGGSLDGTRAIAEIYQSRDDRVRWIDAGPADPQWTGKAWNLQVGLARS
                     NPANRWILCIDADVRVSAKLSRSLISHAASTGVSTFSVATRQHLSGPSEALVHPSLLT
                     TLVYRFGQPGRAYRNIDQVQANGQCFFSPRETLLKAQAFSAARSSLCEDITIVRRLVE
                     RGEAVGFYETHGLAEVFMYGNWREAWENWPRSLPMRDQYFGWRQTIGLLEVFFVQGLP
                     LPMLIFAPNAGASKLFLALNALLLIFRTGVLFGTARAYNRRPWTYWLSPVCDFAVAVR
                     LIQSALSRKLSWRGRSYVRRSAGILEPEGTC"
     gene            complement(33983..34759)
                     /locus_tag="VF182_19795"
     CDS             complement(33983..34759)
                     /locus_tag="VF182_19795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439369.1"
                     /translation="MKTARRFIGRCLVLLSSLMFLSGVSAAQDDNPLPPGMYRLEMIL
                     ASVTRLPFFGMSKSASRSVSLIEVRSDRTGLLQRHEVCDFHVLQDSAMIKMVFPDKFV
                     AALAKHTYPIQVEKDGQGWRYRADLGVERIGYKPSSSEANLPTKLDDPAVYDWDGDGH
                     PGATLKISVPLLPDGELYIVQRGHSILNGRITTRGRVEGSIEVRSFEHRVLGAWPGFL
                     NRSPEIVPDPAQSRFTIIAIPQDSICETLRGSPRQSTQKD"
     gene            complement(34996..35448)
                     /locus_tag="VF182_19800"
     CDS             complement(34996..35448)
                     /locus_tag="VF182_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6439370.1"
                     /translation="MKIYHLRTELWLPQPRSEIFKFFADPSNLERLTPGWLRFEIIAE
                     VPPIMRAGARLEYRLRIHGVPIKWQSEISAWNPPHGFIDRQTKGPYLFWEHEHSFFEH
                     QGGTLVNDNVQYSVFGGTLVNRFFVAPDLDRIFKYRHSALKTIFNPRD"
     gene            35699..36604
                     /locus_tag="VF182_19805"
     CDS             35699..36604
                     /locus_tag="VF182_19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX6439371.1"
                     /translation="MKIVITGASGFIGSRLADHLWRQRHQLILLSRHPPRELNVTQHE
                     WVSWIPGVSGEWVEAIDGVDGVINLAGEPIAAKRWSDVQKEKIRFSRVETTKALVNAI
                     ANTKQKPKFLINSSAVGYYGGRGDEIVTEETAPGSDYLARVCVDWEKEARKAESFGTR
                     VALVRTGIVLDKGQGALAKMVVPFKYFVGGPLGSGHQWMPWIHIEDQVGLLLFLIENQ
                     NARGPFNASAPNPVTMTEFCKTIGTVLNRPSWATVPAGILTLAIGEMAEMLLNGQRAV
                     PQAAAKLGFEFKYGNLLPALESLHL"
     gene            36612..37511
                     /locus_tag="VF182_19810"
     CDS             36612..37511
                     /locus_tag="VF182_19810"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX6439372.1"
                     /translation="MSAINDYQTGQRQRLYVRDLLNGDDEAAFQVIHRMLLARRSLGD
                     VYLSLITPALTEIGQLWCDGEIGVGLEKLASHLVLKHMDRLRGMHTSERQRLPQRILV
                     ACVEGELHCIGARMVADLLDIEGWPVDFLGADVPTASLIDIIKIRRPHLVALSVRTSS
                     GLDHARHVVGEFKTMADAPKILLGGQAMARNESPSDATCAVARNALEGVQLAGKLLNA
                     GRPKAVLKEYLIGLGRRVRDLRNKKGWTQEQLAESARLTRVSIVAIEGGKQNISMDVI
                     LRLANSLATSPEDLLSSHRILRS"
     gene            37524..38642
                     /locus_tag="VF182_19815"
     CDS             37524..38642
                     /locus_tag="VF182_19815"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX6439373.1"
                     /translation="MKNLSLNLSNEVPAGRIFLEQAKNLEHRSIVREGLGSMLPEPER
                     MTQLLGLDDPSIVLDVLRTGLLDPLAEATSTRGKRIRGQLVTLGYRLLTDTPASLLAA
                     KQCGWCAAVVEMIHTGSLVIDDIEDGSSVRRGRPALHLQFGIPIALNAGNWLYFWPFE
                     LLRETELPVDTIRYLYEHCHRTLLRAHFGQAIDLGAPLSSLPQHRVAPVCLTSMKLKT
                     GALMGFASILGGAIARASESVLSILDQFGRDLGVSLQMFDDLGNLIGKCEPAKRYEDL
                     SLSRPSWVWACAAESSTARAYGEFCIAVGSLPDTHKLESWSEAHNLVERVRACAHGYL
                     DDAFRQLKERLDSANVRWSKRAFEDLRELGEEITVAYG"
     gene            38635..40170
                     /locus_tag="VF182_19820"
     CDS             38635..40170
                     /locus_tag="VF182_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HEX6439374.1"
                     /translation="MDKVSAAIIGSGFGGLALAIRLQAAGCRTVIFEKRDQPGGRAYV
                     YRDQGFTFDAGPTVITAPDCLEELFTLAGRSMADYVTLLPVKPFYRLFWQDGFSFDYS
                     ADLADVERQIAIKAPGDLHGYKSFLKYSEKVFEQGYVKLAHVPFLDWSSMAKVAPQLL
                     RLKAYRSVYSIVAKFIRDPHLRQLFSFHPLLVGGNPFTTTSIYTLIHCLERKWGVFFP
                     KGGTGALVQGMLKLFQELGGRIRLQSEVSEILTRGNRVVGITTKTGETHECDLVASNA
                     DVVHTYDRLLRREPKARRMGKRLAKMSYSMSLFLIYFGTRRQYPNLQHHNIIFGPRYR
                     ELLKDIFGRGRLADDFSLYLHAPTRSDPSLAPPGCEAFYVLSPVPHLGKLSVDWSVEG
                     PRYAERILDYLERRYTPNLRNEIVTQRIFTPQDFHSELNAHLGSAFSLEPRLMQSAYF
                     RVKNRDANLTGLYLVGAGTHPGAGIPGVVNSAKATAGLIMDDIKKAVVMRSSSQTQRL
                     SYA"
     gene            40163..41116
                     /locus_tag="VF182_19825"
     CDS             40163..41116
                     /locus_tag="VF182_19825"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEX6439375.1"
                     /translation="MPEAMSPKVKAALEECRWMITKGSKSFSLAARLFDSDTRDAAFF
                     LYGWCRYCDDQVDRAGMAESREQLERRLAKLSEATRSVFSGAAQEQAVFIAMQYIVQR
                     YSIPAHYALELIEGMAMDARGTRYQTFKDLSLYCYRVAGTVGLMMSHVMGLRDEQALK
                     HAADLGIAMQLTNIARDITEDASMGRIYLPLEWLKEADIAPEEIAAVKNRPKLALVTK
                     RLLQEAQRYYLSGDDGLWHLSFRSACAVAAARHVYAEIGSLLLDKGPSAWDQRSYITF
                     ARKLWVILCGVLHVIASMPARLMRPWSAAPIHKVWRQSELR"
     gene            41154..41999
                     /locus_tag="VF182_19830"
     CDS             41154..41999
                     /locus_tag="VF182_19830"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HEX6439376.1"
                     /translation="MHYIPWLFLIVQGAIVVASLLGYGIFTSRPELLIQIDPGARFFT
                     WAFYGFAVGNMLFGGLAVVAEAVLRDRTNALWAFASIYFVSLASELLGTTYGIPFGAY
                     SYTALLGVKWFDRVPLLIPLSWFTMSWACWVIARQRSAALSAVVLATALLVAWDFLLD
                     PAMSKVTSYWIWGQSGTYYGMPWMNLLGWGITGLVLFVILCKIAPAPHTERRFAIWVY
                     MINLALPLGFCVLNQYWLAVFTGVGTIAIALIVFGKKGSILAAGEMEDLSPSGRAGAL
                     SSQRE"
     gene            42123..43055
                     /locus_tag="VF182_19835"
     CDS             42123..43055
                     /locus_tag="VF182_19835"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX6439377.1"
                     /translation="MIPSELSDYLQRQRTAIDRALEQFLKVYGGQTQAILKAMRYGLF
                     PGGKRIRPILTLTAGEMFGAKQKELMPFACAVELIHAYSIIHDDLPAMDNDDVRRGEP
                     TAHKVFGEGMALLAGDGLLTEAFHLISGPEVRRLPAELVLRLIHELSHAAGIAGLVGG
                     QAADLESENKDVDLATVEYIHVRKTGALILAALRTGAQIAGAKTGDLRKLSKFGEYLG
                     LAFQIADDVLDNIGETLAGEGAESGRNELKKATYPSVVGIVQATERLHELLRLCNREL
                     VSFGESAEPLRAIAKDIISRAVQDRGRTAEESIS"
     gene            43056..44972
                     /gene="dxs"
                     /locus_tag="VF182_19840"
     CDS             43056..44972
                     /gene="dxs"
                     /locus_tag="VF182_19840"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX6439378.1"
                     /translation="MANLLDGVEYPKDIRRMTLDELNELAAEIRQEVISVVSEVGGHF
                     ASTLGAVELTLALHYVFNTPEDRIVWDTGHQAYAHKLLCGRRNRLSTIRQFGGLSGFL
                     SRDESEYDVFGAGHAGTSISAALGMVEAKTLESSSRKVVAVISDGGLSAGLTYEGLNQ
                     AGHLEKDLIVVLNDNAIFIDPRVGAVSSFLSKQFTTDVALRLQKNLSALLRTLPKGEN
                     LFHLARKVKESFLGLVTPGFLFESLGFQYVGPIDGHNIEEMIATLENVKKIEKPTLVH
                     VVTTKGKGYPPAEKEPVKFHGVTPFHVLTGKPKKGKGPIPTYTDIFGEALIRLAKENP
                     KVIGITAAMGSGTGIDKLARELPNKSYDVGIAEQHAVTFAAGLATEGFVPVVAIYSTF
                     LQRGYDEILHDVCLQNLHVVFALDRGGLVGADGPTHHGVFDFAYMRSIPNIVIMAPKD
                     ENELQHMLKTAVDYSGPISIRYPRGEGCGVEMDAQMKNLEVGRAELLRHGTDVVIAAI
                     GQTVMPALRAAQDLVPLGIDAAVVNARFVKPLDRELFRDLLLRAPRLITVEDHTVVGG
                     FGSALLEFMAEEGITGVEVRRLGVPDRFVTHGTQDELRKLCGFDKDAIAQSALQMIRR
                     AKRKSKEGWERGSA"
     gene            44951..45697
                     /locus_tag="VF182_19845"
     CDS             44951..45697
                     /locus_tag="VF182_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX6439379.1"
                     /translation="MGKRERLDLLLVQRELVSSREEGRRRILAAEVLVNEQLSTKAGA
                     LVDQTAEVRLKAASAYVSRGGLKLEKALRFFQIEVKDKVALDVGASTGGFTHCLLSHG
                     VARVYSVDCGYGQLDWKLRNDPRVVVLERRNIRYLEASDLPSLAHFATIDVSFISLRL
                     VLPKVKTLISPGGEIIALIKPQFEVGKGKVGKGGVVRSDAEHLRVIDEIEQTAAGLGF
                     TTRNITESPLLGAKGNKEFLIHLTDPGFRS"
     gene            45753..46481
                     /locus_tag="VF182_19850"
     CDS             45753..46481
                     /locus_tag="VF182_19850"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX6439380.1"
                     /translation="MAKFLRIAHRGASGSFPENTQLAFEKAIEAGADMIEMDCQLSHD
                     GHVVIFHDERLVRITGAGGIVNRMPLEQLKRLDAGVLRKSAFKGERIPTLEEVVALVG
                     GEADLCLDIKQYPGSPPGIELKLLFILSHYDYLDRTIFSSFDYACLRRVRELAPEARL
                     GVIYGNGIEENPFAVAAEIEAVSLHVQKELATREFLQNSWDLGLDAFVWTVNEVREME
                     DFASLGVQGIMTDFPERFRHMEKT"
     gene            46685..46879
                     /gene="rpsU"
                     /locus_tag="VF182_19855"
     CDS             46685..46879
                     /gene="rpsU"
                     /locus_tag="VF182_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX6439381.1"
                     /translation="MTGVRVRENESIESAIRRFKKLCEKAGILAELRKREHYEKPSVK
                     RKKKAIAAKKRALRRVRHSF"
     gene            46928..47377
                     /locus_tag="VF182_19860"
     CDS             46928..47377
                     /locus_tag="VF182_19860"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX6439382.1"
                     /translation="MHLKAEIQDSMKSAMRSGDKITLESLRLALAAIKNEEIKFRREL
                     TTEEIQRTIATLCKQRSESIEMFQKGGRSDLVEKEQAELKVLQRFLPEPLSDDEIKSA
                     IRSTIRESGAQGLQDLGKVMKEVMPKVSGRSDGKRVNELAREILRGA"
     gene            47570..48103
                     /locus_tag="VF182_19865"
     CDS             47570..48103
                     /locus_tag="VF182_19865"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX6439383.1"
                     /translation="MNTFDIILLCFLFLFALRGFLKGFLREILSLAGLIVGFMIAARY
                     NDAVAMLGQEYWETSLFVLKGASFVAIFFAVYLCFNLLGWFFHRSERFLFLQTLNRAG
                     GIVIGIGKGAALVALIVYFMSSASWIPASASEKIKTSVLTPPLSRLAEVIIRVGKERI
                     LTTVDNRVRLFSPTRVL"
     gene            48112..49914
                     /gene="dnaG"
                     /locus_tag="VF182_19870"
     CDS             48112..49914
                     /gene="dnaG"
                     /locus_tag="VF182_19870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX6439384.1"
                     /translation="MITQEKIAEIRARASVVEVVSDYVTLKKVGRNHVGLCPFQGEKT
                     PSFTVNDEKGIYHCFSCKAGGSVFNFLMQHDHLSFPEAVECVGKRYGIAVKHLGRNSG
                     SGEEADREALYRLNEAAAANYHRVLFKDVLGKRALEYIKKRGIAEPTARRFMLGFAPP
                     YGSGLLDVIKKEKLSLKDALRLGLIGQREGNRFHEKFFDRLIFPIVNAGGKVIGFGGR
                     VLEQGIPKYLNSSDTPLFRKGSTLYGLFQAKEAIRKVDRAVVVEGYLDVIALHQYGVD
                     YTVATLGTALTVEHVRGLSRYTKNVVAFFDGDDAGSRAAERSFEIFVEAGLLGRAAFL
                     PNGEDPDTFIRSRGKEAAEELLDRSVPLADYYFSRLEQRHGKTLEGKSRIAEEIARLL
                     QKVRNPFEVDLLIARAVDSLGIREEFFRKPLQSAVPAKTPSNAIQRLSASDRSDRGDV
                     AERSLITLILRYPVIAGYVFRDTDVRQWLGSDWREILDLIRADWQEHGNVDVARILQA
                     FESSTAAAIAGLAIEGEQVPEAESQKMADDCIAHLRRKFMREQERNLRIAIRTAEEQK
                     DENATRERILEWQEIVRKERQLERRKLGAKITAR"
     gene            49923..51716
                     /gene="rpoD"
                     /locus_tag="VF182_19875"
                     /pseudo
     CDS             49923..51716
                     /gene="rpoD"
                     /locus_tag="VF182_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237079.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
     gene            51755..51831
                     /locus_tag="VF182_19880"
     tRNA            51755..51831
                     /locus_tag="VF182_19880"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51789..51791,aa:Ile,seq:cat)
BASE COUNT        12216 a        12893 c        14506 g        12386 t
ORIGIN      
        1 aagaccatgg aaggggtcgc gagctttgcc ggcacaattt taaaaagcgg tttcgagaga
       61 aactggctga gccgcttggg ttatttgata agccgagagt ccctgcgaca cgcttaccgt
      121 aagctcgatt atgccgaata cggcggagcc cgtttgttgg gattggatgg ggttgccatt
      181 gtcgcccatg gagggtcaac tgcgctggcg atcaaaaacg ccattcgcgt cgccagcgaa
      241 tccgtttcac aggatgtaaa cggccatatc agtgcggctt tggaatcaat cgttcaggcc
      301 gaaactgaaa gcaaagtttt gaagactacg attgggcctt gatcgagcca ttgtgcgatg
      361 gggctaactg gagcgaaggc acaagtggcg agtacgaata gtgtggcatt tctttttccc
      421 ggtcagggct ctcagtatgt cggcatgggg aaagaattgt gcgagcactc ttcagttgcg
      481 gcgcaagcct ttcaagaggc cgaacagacc cttgggtttt cactcaaaca gctgtgtttt
      541 tacggtccgg aagccgatct ccaactaact gaaaacacgc agccggccat tttggctgtt
      601 tcggtggccg cgcttcgcgt tttgcaagcg gaaatcgcgg ttcaaccggc gtatgttgcg
      661 ggtcatagcc tcggcgaata cagtgcgcta gtgtgcgtcg gcgcgctctc gttcagcgat
      721 gccctgaaaa tcgtgcgcga gcgcggtcgt ttgatgcaag cggcggtgcc atcgggaaaa
      781 ggcgccatgg ccgttatcat gggtctcgac gtggcagcgg tcagctctat ctgtaaacag
      841 gcggaagacg gggaagtggt cgcacctgcc aattataacg gtggcggtca aatcgtgatc
      901 gccggatcca aggccgcggt agcccgcgct atgcttcttg cgaagtcgga gggcgcaaag
      961 cgtgtagttc cgttgccggt cagtgcgcca tttcactgtc cactgatgaa gccggcagca
     1021 gaaggactga aacgtattct aaacgatgtg acgattcatc cattcacggt tggagttatc
     1081 ggcaacgtga atgccgacgt gaatctcgat cccgctcaaa tcaaactatt gctggcggag
     1141 caggcgatga aacccgtacg ctgggaggaa tcggtccaaa gattggaaaa tctgggcgtc
     1201 tccaaaatgc ttgaagtcgg tcctggaaga gttctaaaag gattggtgaa gaggatcagc
     1261 ccgaaaatag cggtggatac cttcgaagtg ccgcaagacc ttgcccgatt aaggaatgac
     1321 atcagatgaa atcaaacggg caagtagctc tggtgacggg tgcaagccgt ggcatcggtc
     1381 gggctatcgc cgtggctctc gctcaagcgg gagcatacgt ggtcgttaat tatcgcggca
     1441 accaagaggc cgctgaaaca acgctcgaag aggttcgggt cgtaggcggg caaggggaac
     1501 tttgccagtt cgatgtcgca gtcgaaactg agatcgatgc ggctgttaag aaaatcgttg
     1561 acgggcaagg gaaaattgat attctggtca acaatgccgg ggtgacctct gatactcttc
     1621 taattcgcac aaaggcccaa gattgggatc aagtcgtaga tactaatctc aagggaacgg
     1681 ttctctgtac gaaatcggtt tgtcggctca tgatccgcca gcgttatggc aggatcgtta
     1741 atctttcttc ggtagtagga cagaccggca atgcaggaca gtcgatctac gcggcaacga
     1801 aggcgggaat tctcggcttt accaagtcca ttgcgcggga actcgcgtcg cgcggcatca
     1861 ctgttaatgc ggtggccccg ggatttatcg aaaccgagat gactgctact ttatcgccgc
     1921 aactgcagga ggagtttctg cgctcgattc ctttgggtcg tttcggcaaa agcgaggaag
     1981 tggcgcagct ggtggttttt cttacgggat cgggagcgtc ctatatcacg ggtcaagtga
     2041 tgaatgttaa tggcggactt tacatgtagc aagaatggcg aataagcagg aggaaaacaa
     2101 aatggcgtca tctgtgcaag ctagggtaaa agagatcgta tgcgaacaat tgggggtaag
     2161 cgaggatgag gtggttcctg aagcatcttt catcgaggat ttgggcgcgg attcgttgga
     2221 catcgtcgaa ttagtgatgg ctttggaaga agagtacgag atggagattt ccgacgagga
     2281 cgccgaaaaa attaaaaccg tccaggacgt catatcgtac atcgacaatc atcgttgagc
     2341 gttgcgttgt ttcagatcct tttcgggagg agatgatgca tgcgccgctc gagttttcgc
     2401 catttaacat ccaggctcgt aggagtttac aagaaccctg caagctcgcg gtttagcgga
     2461 gtccattatc aagaactgtc atgattgtga tggctagtga tcacggcggg gtggaactca
     2521 aagatttttt agcggaagtc cttcgttcaa aagacattga tctgcaggac ctcggtacct
     2581 acgaccgtaa accggtcgac tatcccgatt ttggtcaccg ggtgtcggag cgagtttcac
     2641 aaggagatgc aaatagaggc atattaatct gtactactgg aatcgggatg tccatcttgg
     2701 ccaacaagta tcccaatgtc cgggccgctt tggtgcagga tcgtgaaaca gcgcgcagca
     2761 gtcgcgagca caatgacgcc aatattttag ttctcgctgg ggccaccacc gaccctgccc
     2821 tcgcccggga gattttggag atctggatca acacaccttt tagcggcggc cgacatcaac
     2881 gccgtcttga taagataact gaaattgagc gagccttggg aacactgaga aaggcgcccg
     2941 ccagcaagag cgacaaggat tctgagtgat gtcttttttg cagcagaccg accccgaggt
     3001 ttttgccgcc atccaaagag aaacgaaccg gctcgaacac aatttggagc tgatcgcttc
     3061 tgagaatgtc gtgagcgaag cggtcttgga agctcagggc tccgtaatga ccaataagta
     3121 tgcggaggga tatccaggca agcgctacta tggtggctgt gaatttgtcg atgaggtcga
     3181 atctcttgcg atagccagag ccagagaact cttcggcgca gatcatgcga acgtccaacc
     3241 tcattccggc agccaagcta acatgaccgt atatctgtcc gtgcttaagc cgggcgattg
     3301 ttatctcggg atgaatcttg cgcatggtgg ccatttgtcg atgggaagcc cggtcaactt
     3361 ttccggcatt ctttaccgtg tcgttcctta tggggtctca aaagaaactg aaatcatcga
     3421 ctatgacgaa gtcgccaaac tcgcccgcga acatcgaccg aaactgatca ttgccggcgc
     3481 cagcgcctat ccgcgaatca tagactttgc aaaattccgc caaatcgcgg atgaggtggg
     3541 ggctcttttg atggtcgata tggcgcatat cgccggttta gtcgccgcgg gtttgcatcc
     3601 gagcccggtg ccgcatgccc atttcgttac aaccacgacg cataaaactc tacgcggacc
     3661 tcgcggagga ttggttctgt gccgtgccga gtatgccaaa accgtcgaca gcaaagtctt
     3721 cccaggcatg caaggcggtc ccttgatgca cgttattgct gccaaggcgg tcgcgctgaa
     3781 agaggcgctc aatccagcat tcaaaatata tcagcaccaa atcgtcaaga acgctcaagc
     3841 cttggctaaa tctctcagct ccaacggcta tcgcctcacg tcgggaggaa ccgaaaatca
     3901 tctgatgttg gtagatctgc ggaaatcgga gctgacaggc aaggtggcgc aggagaccct
     3961 cgatcgggcg cacattaccg tgaaccgcaa cgccgtaccg ttcgatactc ggtcgccatt
     4021 tatcaccagc ggaattcgcc ttggtacgcc cgccgtcacc agtcgtggca tgaaggagga
     4081 ggaaatgggc ctgatcggta acttgattgc gagggcgctt gaccgcgcgg gcgatgaaaa
     4141 cgttaggagc gaggttgcga acgaggtggc cgcgctgtgc agaaaatttc cggtttatcc
     4201 gcatcggctt ccgacgaatt aatctgaccg gtacagattc aacctgcgcc agtcccgcca
     4261 tttactctct gcggtcctgc gacaatctat tcctagttta catgaagtga actagttgac
     4321 tgtgttcaaa ggaacttcgg atgaaatgcc ctttttgtca tgaaaccgag aaccgcgtga
     4381 tcgattcacg cttgagcaag gataacaata tgatccggcg ccggcgcgag tgctccaagt
     4441 gttcgcggcg ttttaccacc tatgagcggg ttgaagagat gatgccgttg gtggttaaga
     4501 aagatggtcg aagggagact tacgagagga ttaagattat caatgggctt aaacgagcct
     4561 gtgagaagcg tccggtgagc atcaatacga ttgaagcgat tgccgatcgg attgagcgca
     4621 atcttcagga acgcggggaa aaagagattt tgagctcagt gatcggcgag gcgactatgc
     4681 gggaacttca cgataccgac gaagtggctt acgttcgctt cgcctcagtc taccgctcgt
     4741 tcaaagatat caatgagttc atggtcgagc tggaggagct tttaaaggaa cggaaagaga
     4801 tgtcagccgc tcgggtaagc gctccgcgac aaaagaggaa ctagccggtg gtgctgccgc
     4861 gggaagaact tgaccggcgc tatatacgga cggcctgccg gcttgccgag aaagccgccg
     4921 gaaggaccag ccccaatccg atggtgggcg cagtgctggt gcgccagggg aaaatgatcg
     4981 cgtccggatt tcaccaattt gccggcggcg atcacgccga aatcgttgcc ctcaaaaagg
     5041 ccggcagtaa ggccaaggga gccacgctct atatcaacct tgaaccatgc aaccattacg
     5101 ggagaacacc gccgtgcact cgctctttga tcgccgcagg tgtcaaggaa gtggttgtcg
     5161 gcatgaaaga ccctaatccg ctggtatccg gcaaaggact gcgcgcgatg gcccgcgcag
     5221 gaatcaaagt tcgcagcggt gttctccaag aggaatgtcg agtactgaat gaggcattta
     5281 ccaagttcat cactcgcgga gtgccgtttg tgacgctcaa gctggcagct tctctcgacg
     5341 gtaagattgc tacaccgaac ggcgattcct attggattag ctgcgaagaa tctcgtgcta
     5401 tcgtgcatca attgcgcaat agcttcgatg cggtgctggt cggggcgggc actgtgatta
     5461 ccgacgatcc acaactgact tgccggattg cgggcggacg agatccccgg agaatcatcc
     5521 ttgatgcgcg tttgcggatt cccctggacg cggaatttct gcgccagcgc ggcagcgata
     5581 aaaacattat cgtgacaagc cataccgcgc ccgcaggtaa gattcgagct ttggaaaatt
     5641 taggcgccac agtttggcgc ttgcctgtgc gagaaggcgc agtgcggtgg cttccactgc
     5701 tgagaaaact ggccaaaagc ggcattgtca gcgtactgct cgagggcggg gcgattaccg
     5761 ccgccggcgc tttgaaggag aagatggtcg acaaagtcct gcttttctat gcccctaaac
     5821 tcctgggcgg cgacgcacgt tccatgattg ctagtttggg agtggaaaga atcgatcaat
     5881 ccatcatgct tcaaaatctt cagattcaaa aatcaggcac cgacttgctg gtttcaggat
     5941 attttaatac gagcgcggct catgaccgag tgcgctaagc tgaaagattc gcctggatca
     6001 tgtttaccgg tttgatcgaa gatgtcggac gcgtcgccag ctcagtctta aacaagcagt
     6061 cggcggtttt gactgtggag accggtctgg ctcttcgcga gatccctctg ggcgccagca
     6121 tcgccgtcaa cggcgcctgc ttaaccgtcg ttcgaaaagg caaaagatcc gtggcatttg
     6181 atgtttcgcc ggaaacaatg gagcgcacca gtttagctac ggttgaaccc ggcacattgc
     6241 tgaatctcga acgttcgatg cgactcaaag atcgcctggg cgggcatttg gttaccggtc
     6301 atatagacgg tgtcgggatt gtgcgctcgg tgcaaagaaa aggagacttt acctttgttt
     6361 cttttcggat tgctcaaagt ttgggatcac tgctggtctc caaggggtcg gttgctgtcg
     6421 atggaatcag cctgacagtc aaccgatgta acgcacggga gtttagcgtc gcgattattc
     6481 cgtttacttt ccaaaacacg aatttgcgcg cccgtagggt tggtgataaa gtcaacatag
     6541 aagctgatct aatcggcaag tacgttcatc atttcgttac gcaacgaact caaaatccac
     6601 tttaacgttc gaatatcgcc gctaggacca tctcatgccc gtttccagta tcgatgaagc
     6661 tctcgaagac ctccgccaag gccggatgac tatactcgtc aacgaggagg cgcccgacag
     6721 cgacgggttt ttttgcattc cagccgagaa ggtcactgcc gagatcatca atcaaatgct
     6781 gcattacggc agaggcgtta tttatctcgc actcaccgac gagcgaattc gccaactcgg
     6841 catcctgctg atcccgcacg agagcagcaa cttctcgggg catctgttcg gagccgcctt
     6901 ttccgcgcgt ctcgaaggtg ttcccgctgt ctccgccaaa gggcgcgcgc acacgattca
     6961 agtggcggtt gccgatcaag caaagcatgc ggacttaatt gtcccaggac atatccagcc
     7021 gatccaagca cgctccgggg gagtgttggc gcgttcgggg cgagcggaag cgtcgatcga
     7081 tttagcgcga ttgtgcggat ttactcctgc aggggtgatc tgccacatcc ttaaagatga
     7141 tggttccgtg gcgctgttgc cggatctgga gaagcttgcg gagcggctgg agataagagt
     7201 cgtcgccgtg acccgactga tcgcctatcg cttacgcaat gagagcttgg tcaaacgagc
     7261 cgcggaggag atctttccga ctatccatgg cggcatgtac aaggctatcg tttatcgaaa
     7321 cgaagtcgat gcttccgagc atatggcgct ggtcaagggg gatatcagta atccggacag
     7381 cgtcctggtg cgtttgcatt cggaatgctt gacaggagat gttttcggct ccgagcgctg
     7441 cgactgtggc gatcagatcc gccagtcgct gaaactcatc gatcaggctg gcaagggcgt
     7501 gctcgtatat atgcatcaag agggtcgtgg tataggtctc accaataaga tcaaagcata
     7561 tgccttgcag gatcggggct ttgatacggt ggaagccaac ttggagctag gattcaaaga
     7621 agatctccgg gattatggga tcgccgctca gatgctgcgg gatatgggcg tgaaaagcgt
     7681 tcgcctattg actaacaacc cgcgcaagat ttcgggtatc gaagagtacg gggtaacggt
     7741 gaaggagaga gttcctcttg aggtattgcc acgcgacagc aatattcatt atcttcggac
     7801 caagcagaaa aaactcggcc acctgttaac caatttggaa gccaagccag gcaaatctta
     7861 acgctctttt atggccaatg tcattgaagg cagtattgac gcccgcggca tgaaaatcgg
     7921 tgtcattgtc agccgattta ataaatttat cactgccaag ctgctcgaag gcgcattgga
     7981 gggtctgaaa actcatggtg gtgccgatga tgatctgacg gtggttcatg tgcccggcgc
     8041 gtttgaaatt cccatcatcg cggcacaaat ggcggccagc ggaaaattcg atgccgtcgt
     8101 gtgtctgggc gctgtgattc gtggcgatac gccgcacttc gaatatgtct gcgatgcggc
     8161 aacgcatgga atcagcaagg ccgtctggga ataccggata ccgatcagtt tcggggtcct
     8221 gacaaccgat aacgtgcaac aagccatgga acgcgctgga gtgaaagata cgaacaaagg
     8281 atatgaagcc gcgttaagcg ccatcgagat ggtaagtgtg ctgcgccaac ttgctcgata
     8341 acattcctgg attatcttcg tcgagggcgg gcagccaacc tttagagatt cataggattc
     8401 tgttagtcat gggaatcaga cgcaaaggca gggagttggc ccttcaagcc ctgtaccaga
     8461 ttgaaattac cggcgacgct tcagcggcgg cggttgatct cttcctcagt catttcgaag
     8521 gcagtgcgca agctaaagaa tttgcacgac gtttggtctc cggcgtcgtc agccagcggc
     8581 cgaatattga ccgggtgatc gaccactgca cggaaaactg gaagcttgcg cggatggcca
     8641 aagtcgatct gcttattctt cggatatccg catacgaatt aatcttctgc cccgacattc
     8701 ccctcaatgt cagtctcgat gaagctatcg agatgggcaa acgtttcggc agcggtgatt
     8761 cgggctcgtt tatcaatggc gtgttggatc aactggcgca cgcagagatt gccaaagctc
     8821 actgactgca tggaaccgcg ttaccaagcc aaagaaattg aagagaagtg gcagactttc
     8881 tgggaggagc atcagtcctt tcaagcctcg gaagctccgg gaaagcctaa gtactacgtg
     8941 ctggagatgt ttccttatcc ttccggtcgt attcacatgg ggcatgtgcg taattactcc
     9001 atcggtgatg tggttgctcg ctacaagcgc atgcgcggat acaacgttct tcatccgatg
     9061 ggatgggatg ccttcggcct gcccgccgag aacgccgcta tcgagcgcgg aattcatccc
     9121 gaaaagtgga ctcaggaaaa tattttgtac atgaaggcgc agctcaagcg catgggcctg
     9181 tcctacgatt ggtcgcgtga gatcgccact tgccggcctg aatattaccg ctggaatcaa
     9241 tggatatttc tgcagttcta caaacgaggc ttagcataca agaagagttc tttagttaat
     9301 tggtgtcctt cctgcgagac ggttctcgcc aacgaacagg tgattgaagg cgtctgttgg
     9361 cgctgtgaat cgacagtgac tcagaaagaa ttggatcaat ggttttttcg catcacagac
     9421 tacgccgagg acctgcttca cgatctcgaa cggctaccgg gttggcccga caaagtactg
     9481 ctgatgcagc gaaattggat cggcaaatca gtcggggcag aaatcaattt tccgcttgcc
     9541 gggcgagccg aaccgctgaa agtcttcacc acccgtcccg ataccgtatt tggcgcgacc
     9601 tttatctcgc ttgcggttga acatcctctg gccttgaagc ttgctcaagg attgccgcaa
     9661 gaagatgctg ttcaagaatt tatcgacacg ataaaacagc agaagtcgat agggcgcggc
     9721 ggtgaagaag gtgaaaaaga aggcatcttc accggagctt attgcacgaa tccattcacc
     9781 agcgagaata ttccaattta tctagcgaac tttgtgctga tggagtacgg caccggcgcg
     9841 gtcatggcgg tgcctgcgca cgaccaaagg gattttgaat tcgcgcgcaa atacggattg
     9901 ccggtccgag tcgtgattca acccgcaggc agtgttctgc gcgcgcaaga attgacctcg
     9961 gcgtatgtcg atgaaggcgt gatggtcaat agcggagtgt tcagcggatt gaccaacatc
    10021 gacggaaaag aaaaaattgc cgaacatctg gagaaacaag gatggggtaa aaaggcaatt
    10081 cgctaccgcc tccgcgattg gggtgtctcg cggcaacgtt attggggaac accgatacca
    10141 attatctatt gcgacgcatg tggtgtcatg cctgtccctg aagatcagct tcctgtcacc
    10201 cttcccattg atgtgccgtt taccggcaaa ggccgctcgc cgctggctga aagtaaagac
    10261 tttagtgaag ttcaatgccc tcgatgcggt gccgccgctc gtcgcgaaac cgataccatg
    10321 gatactttcg ttgattcctc ttggtattac ttgcgttata cttctccgaa atcgttcacg
    10381 actccttttg atgccaagca ggcggcatac tggatggcgg tggatcagta tatcggcggt
    10441 gtcgagcatg ccgtgcttca cttactctat gctcgatttt ttaccaaagc tctgagagat
    10501 cttggggttc tccaagtgga tgagcctttt accaatctcc ttactcaagg gatggtttgc
    10561 aaagaaacct atcgctgccc tgagcataac tggctcctgc ccaccgaact tgccggctcg
    10621 gaaaaggacg gatggcgctg ccctcaatgc gcgcgtctag tcgaaaaggg tcgcgtcgaa
    10681 aaaatgtcca agtcgaagaa aaacattgtc gatccggaag agcttgtcgc agcctatgga
    10741 gccgacacag ctcggctatt cacgttgttt gcggcccctc ccgagaagga tcttgaatgg
    10801 agcgagcaag gagttgaggg cgcgtatcga tttctgagcc ggctgtggcg ctttgttttt
    10861 cataatcgag aaatgctgct cggtggtgaa accgattcgc cggagttaaa cggatcctcg
    10921 gatgtgcgcg agctgcatcg catgatccac cggacgatca agaaggtcac cgacgacatc
    10981 gatggaaggt tccacttcaa taccgcgatt gcggctatca tggagctctt taacgcgctc
    11041 agtatcgccg ccggccggca ggaaggacag ttggctgctc cggttatccg gaccggcatg
    11101 gaaaccatca tccggctcct ctatccattc gtgccacatt tgatgagtga actctggctc
    11161 agcctggaaa aaagccagcc actggatcaa ctatcctggc cggagtattc accggaggcg
    11221 ctcgacgaag agcaatttct tatcgtcttg caagtcaacg gcaaagtgcg cgggaaaata
    11281 aaggtccctg ccaatactca aagagatcaa gttgaaagtg acgctctggc ggagccgaga
    11341 gttaaagctt ttatcggcgg gaaaaaggtt gagcgaatcg tttatgtacc gcggcggctt
    11401 gtcaacattg tcttggaggg atgaaattga gggatactct gcgggaaact attagacggg
    11461 caactgcctt gacgatcctc ggttattttt gcgccacttt gaccatggct ggctgcggct
    11521 attcatttgc cggtcggagc gagacgtttc cggacgacat tcgtacggtt tacatcgaac
    11581 cgttcataaa tcaaacgcgc aacgtcggtt tggagaaaga gctaacttca gcgctgcgca
    11641 gcgaatttta tcgccgtggg caactccggg tcgtcgacaa tattgatcag gcggatggga
    11701 ttgttgcggg agtggtgcgg acattcgaaa gccatgtcgc ctcggtcaac cgcagggatg
    11761 aagtgctcca atatgaaacc gctatcgtca tcgatgcgtc gctgcgtcga cgagagccga
    11821 acgaaatcat ttggcggagc caggggacgc gcctgacgga catctatggc ggctcccgag
    11881 cggcggtggt gacaacctcg tcggaattca aaaccgggac gttgaatgca tccgacgttc
    11941 gccggctgac cgatatccaa ttaaccgaga cagaaaaccg cgcggtgcgg ggacaactgg
    12001 tcgaacgctt tgcgcgagag ctgcatcaaa gattgatgga aatgttctag cgccgacaaa
    12061 ctggtcggcg tattctacga cacggcgatt cgattgctat gctgaagaag cggtgcgagc
    12121 ggccgcttgc gccagtcggg aaagccaccg cgaggcggta tttcgattga tgattccttt
    12181 gctcacagcc ttgccgagcg ctttatcgac cgctttgatg tcgacggccc gggaatcggc
    12241 agtctgattt gagactgctt ggcgcgcttt tttgatcaaa gtccttacct tggatctcgt
    12301 ctcctggttt cttgcctttc gtttcagact ttgcctgtgt ctcttaataa ccgacggatg
    12361 aactgccaaa gctagcctcc taaaaatgtt tgattttact taccatcttg gaatgccgga
    12421 gtcaatttga acgaggaagc cgggaagtta acacgggccg ccggagtggt gggcttcttc
    12481 acattgctca gccgcgtctc tggcttgttt cgtgatgtcg tcatcgggtt tctctttggc
    12541 gcgcagggtg ccgccgatgc atacttcgtc gcctttagaa tccccaatct tttaaggcgc
    12601 ctgacagcgg agggcgcttt gacagtcgcg tttattccgg tctttaccaa ctacatggca
    12661 aaggagggaa gggccgaagc tattagagtc actcaaattg tgtttacgtt tttaactata
    12721 tttttgggat tgattacgct actgggcatc gtttttgccc gcccgcttac gcagtttttc
    12781 gctcctggtt ttgtccagga gcctgaaaaa ttctacatgg cggtgtcgct tacccgttgg
    12841 atgtttcctt atattttttt tgtcagcctg gttgcattag ccatgggagt actcaatgcc
    12901 ctccggcatt tcatggcgcc agccatgtca ccggttctat tcaatctctg caatatcatc
    12961 tgcgcgattg tgttttatcc gttactggat gagccgattc ttggcttggc ttttggagtt
    13021 ctcatcggcg gagcggcgca acttctattt cagatacctt atttgttgaa acaaggtatt
    13081 cgtttacggt ttaacttcga tttcagacat cccggcctgg ggcgtttgtt gtatctaatg
    13141 gggcctgcgg cgtttggcgc agcggtctat caaatcaatg ttctggttag cactatgctg
    13201 gcttcctttc tgccaagtgg aagcgtctct tatctctatt atgcagatcg atttttagag
    13261 tttcccgtcg gtatcttcgc gatagccctt ggcaccgcag cgctgccaag cttttctagc
    13321 ttggtggtca agggcaacat ccaagaactt cgcgcgactc tggcgtatgc ccttcgaatc
    13381 gtcaatttca tttgtttgcc tgccaccgcc ggcttagctg tcttggccgt tccgctattc
    13441 gcgatctttt ttcaacgagg cgcattcgat gctactgcga ctctaaactc cgcccaagcg
    13501 ctcatttttt tttcatttgg actctggagt atctcaggca cgaaattagt cgcgccggtg
    13561 ttctatgcta tggaggacac gaaaactcct gttttagtcg gaatcatcgc atttatggtc
    13621 aatcttcttg cgagccttgc gctgatgggt gagattgtcc tcgaggcaga ttccgctgga
    13681 atcatggctc gaacgatggc tatggcatca gttcgtatcg gattgagttc gctgggtcac
    13741 ggcggccttg ccctagctac gacaatctct tcgttatgta attttttcat cttgctcctt
    13801 atcctaaact cgcgcctaaa aggaattcca ttacgcgagg tcgcttactc gtttttccga
    13861 aacctcatca cgaccctgat tatggctatc ccgcttattt gggttgttgg gaatgtcgac
    13921 tggacaggca cgacattgcc gatttattcg aaagccttga ttctcgccgg cctgatcgtt
    13981 ctcgggctta ccttgtatct cgcgttcagt tacctgctca agagtcctga atggccgatc
    14041 gtggaacaat tccggcgcgc gctacaatcc cgcttatgcc gactaacacc taaacgcaga
    14101 ccagaagggt aaaaagactg ctgtcaaact gggcattctg ctacgatccc tgatttgtcg
    14161 ccgccacagg ccgattgacg ttcggttatc aacagcttat gcacaaaact gtggcaaacc
    14221 gattctgttt accctacgtc gcggcggcag cttgcgcctg ccgattaaca aagagagagg
    14281 ttctcccgtt aactctcgtc ccattatctt ttctcctgtc aagcaaaact cgagagcttg
    14341 tgatgcggca atgagacggt ggacgcaaag ttgtcgaaac ctggtctgtt atgatcgctg
    14401 actcgttgcg gcgtatgcgt tcgaagagct gaaagccttg catccgcgac agagttatgt
    14461 aatagacttt gcttagttta aacttttttt atgtgtgacg ggaaaggagg tgaactaatg
    14521 atcggtaaaa aaattgtttt attgtcaggg atatccctct tcactttgac tctcgcaatt
    14581 gcagggtgcg gcaagaaaga agagccggcg ccaccgccgc ctccgcctcc accagctgca
    14641 gagccagcgc cggctccggc ggaaccggca ccagcagctc cgacaccggg tggcgataca
    14701 ggggctccag caacgagtga aaaagctcct gaaacgaagg acgaggcgaa aaccaagtaa
    14761 ggtcttaact cctaacaggt tattgccaac caaaaagggg aagggtagtt atccttcccc
    14821 tttttgtaat ttccgtccct ggaaggcctg tggcatcgga cagttattta actttttcag
    14881 agcatcttga agagcttcgc tggtgtctac tgagagcgct ggctgcgatt gttgttgcgt
    14941 cattggtctg ttattttttc gccgatgcga tttttgcgtt tatggtggct ccgctcaagc
    15001 agattttacc cccgggtcaa aatataattg gaacgaccgt caccgaagcc tttttcactg
    15061 aaatcaaagt cgcgtttctg gcgggcgtgc ttttttcatc tcctttcatc ttttacgaaa
    15121 tatggcggtt catcagaccg ggcctgatcg gcaacgaagg gaggttggtc atcccgttcg
    15181 tcgtatgcgc gactctattt tttctcggcg gcgcctattt ctgttaccga attgtccttc
    15241 ccgtggcgtt ttcctatttt atagaacagt acggcacact cggggttact ccggcaattc
    15301 gcttgggcga gtacttcact tttttttttc gtatggtcct ggccttcgga atcacctttg
    15361 agctgccaat tatcacattt gttctggtcc ggctcggagt ttgggatcac cgcttcatgc
    15421 gacggtcctt tcgttatgcg gtcattgtga tattcatact ggctgcgttt ttaacgccga
    15481 cgccggatat cattaaccaa ttgttgttag cggtgccgat gctgctgcta tacatcctga
    15541 gtatcggcgt cgcgtatatt tggcgtaaag ccgagtgagc gtagtttatc aggtataggg
    15601 aagaggagga ttgatggttg catttgtcat cgccataagt tttttatcgc tgggagttgc
    15661 cgtctttcta gcgcgttggg tactggcgcg cgaaacgggt accccgaaga tgcgcgagat
    15721 ttccgatgcc atcaaagaag gcgctgaagc ttttctctca cggcaaaacc ggacgatcat
    15781 atctatggcc gctattttgg cgacagtgat tttttttcta tacgcctatg ttcgcgcgcc
    15841 gagtcccgca gatccggttt ctcctgtgga aatggccttt tggacaactt tttctttcgt
    15901 tctcggcgct ctttgttccg tgatcgccgg ctatatcggc atgtgggtgt caattcgagc
    15961 aaatatccga accgcttcgg cagttcgcac cagtctgaac gaagggctgc gcattgcgct
    16021 tcgcggcggc gcggtgtcgg ggttattcgt cgtcgccatg agtctgctcg gcgtcggagg
    16081 cctttacgca ctccttgagt tcctcggaca cgacccacag aagattccat tcactattgt
    16141 cgggtacggg tttggcgcat cctttgtggc gctttttgct caactagggg gcggcattta
    16201 caccaaagcg gcggatgtcg gcgccgacct ggtcggcaaa atagaagcgg gcatccctga
    16261 ggacgatccg cgcaacccgg ctgttatcgc agatctagtt ggcgataatg tcggggactg
    16321 cgccggccgt ggtgctgatt tgttcgaatc cactgcggcg gagaatatcg gcgcgatgat
    16381 ccttggagtg ggcctctatc cgtacttcgg catcaaagga atcgtttttc cgttggtcgc
    16441 ccgcgcactc ggtctcctcg cctcgatcgt cggtatcatg atcgttaaaa ccgatgagaa
    16501 cggcgaccca atgcacgctc taaatcgggg atattacgta accagcgggt tggcgatggt
    16561 cggttttttt gtttgcgctc aatggctcct cgctgtcgat ccgacgaaat atccagaagc
    16621 ccagtcggcg tggttttatt tctttctgtg cggcgtcatc gggattctta tggcgcaggc
    16681 tttcgtctat atcacgcaat actatactga atataaatat cgcccggtta gagaaatagc
    16741 cgaggcgtct caaaccggac cggcaacgaa cataatcgcc ggaatcgcgg tgggctttga
    16801 gtgtgtggcg gcgccggttc tggtcatctc ggtcgcgatt attgcttctt actatttggg
    16861 gttaaacagc ggagtaaaaa atgcgggcct tttcggcacc gcggttgcga caatgggaat
    16921 gcttggaacc gcggcatata ttcttgccat ggacacattc ggtccgatca ctgacaatgc
    16981 cggcgggatt gtcgaaatga gcgggcagcc ggaggaggtc cgtcgtaaaa ctgacaggct
    17041 cgatgccgtg ggcaatacta ctaaggcgtt gaccaagggt tacgctatag gatcggcggc
    17101 tcttgccgcg ttcctgctct tttcggctta tctggacgaa gtggcccact atggtcatcg
    17161 gctcgaagcg gtcaatatag ccaaacccga agtatttgtc ggcgcgctgc tgggggcaat
    17221 gctggttttt cttttttctg cgtacgcgat ccgcgcagtg gggaaagctg cgtactatgt
    17281 aatcaacgag gtgcggcgcc agttcaaaga aaatcccggc atccttcagg gcaccgtaaa
    17341 acctgattat cgccgctgcg tcgatatcgt tacagtgggc gcgctgaaac agatgatcct
    17401 ccccggcgtt gtcgccgtga cgatgccgat tgccgtcggc ttagtgtttc gcttttttgg
    17461 cgtcggtgcc gaagccgtcg cggcatttct aatggttgga accatagctg gtattctcgt
    17521 cgctaccttt ctcaacaaca gtggcggagc ctgggacaat gccaagaaat atatcgagac
    17581 cggtgcctac ggcggcaagg gatcggaacc gcacaaagct gcggtggtcg gagacacgtt
    17641 aggtgatcct ttcaaagata ccgcgggccc cagtttgcac gtgctgatca aattactggc
    17701 gacgatcacg ctcgtgctgg cacccctttt cgtgtaagcg gttgtttgag accgtcttag
    17761 ccaggttaaa tcgtgacggc cgagcatacc gtctattgat aggagccatt gatcgaggcc
    17821 ttttaatgtc gcccggatgg tggaatttgg tagacacacg ggacttaaaa tcccgaggac
    17881 cgaaaggtcc gtgccggttc gagtccggct ccgggcaacc acccgagcct ggaacgcctt
    17941 tgcgcgtttc tcaggcgacg gcttagctcc taaagcgcca cggcgggact gctttggaaa
    18001 ttcacctcgc tttgggtctg accagaacca agaaagtccg gatgtccttg cctcgaagtt
    18061 ttcgagtgtg aaagggaggt agttttatca tgcctcagta cgaaatccac gtactttgca
    18121 atgaatgtgg tgccgaacat ccgatgcgtg ttcgtctaca cctggatgac gggccggcag
    18181 aaaagcgtag tatcggtgaa acttacaagg gaagaaccca gcctccgcag cttctcgccg
    18241 tgggcggcca caagactttg tgcctgaaga ccgggaagat gtttgtgcag gaaaacgagg
    18301 atcagatctt cttgatacct cgataatgcg caatccaatc cgacgagtag ctcccgattc
    18361 gtctttgatg ttcctaaaga tgggacgtaa catccgccta gcgtcgcagt tcgtagacaa
    18421 gcaatcgaag tgtgtttgct cagtcgggac atttccaggt atctcggtgg catacggttt
    18481 gcgcaagttt atcctaaatt gaatcttggc agcaagaaaa tcagggaggg cttgctatga
    18541 acgaagacat tttctcagga aaatggagag agatgcgcgg gacattacgc tcttggtggg
    18601 gccggctgag cgatgatgat ttagaaagga tcgctggaga gaaagataag ctggttggtc
    18661 tggtacaaga aagatacggc caaacgcgcg agcaggcgga gagagaagtt gatcgacgtc
    18721 tctccgagta cggaactagt ggtggtggaa tcggttccaa cctgcgatcc agggttgacg
    18781 acttgggcgc tactgtgtct aacaaagcgg gcgaggctgc gggggccatg gccagcggcg
    18841 tgcaatcggc tcggtcttat ctgcaggagc gcgatttcga tcaattgacc ggggacatag
    18901 ttgcatgggt tcgtcggtac cctattcaag cctgtttgat cggcgtcggc ttaggctact
    18961 tgatcgcgcg tactatcggc agcagtgacc aatcagattt cgaataaaac ctgatcgact
    19021 cgcgcagttg atcagctaat cctgccatta tatttgccag atgatgcccc gatcgcgctg
    19081 cgacacgggg catctttttg tcaacgtaag cggcgctgcg cgaatcaaat tcgatccagc
    19141 cattgcgact tcgaacttgc catgctcaaa cgatcgtcga tcccgttata aaaaaaggtt
    19201 cgaaatgctc gactatccaa cgggagactt tgagcatcag ctcacaaact ttcaaaacgt
    19261 taccggatat gtcatagaca ggaacgatcg cgacttcgca taccggtaaa tcgcggtcgg
    19321 agaaagacgc agtttatacg cctggcgcgc gttgtttgcc atcgaagcaa cgcaagaaaa
    19381 tcttcgaacg gctgacggtg ggttgaaatt tgagagtact aaggaggtgt ctgtggcttt
    19441 tgcgaagatc ggaataatga gcatcggcga aatgggctac cactgggcaa agctgttgac
    19501 agctcgtggc gccgaagtgc tgacctatga tcgcgaccgt agcccggtca cacagaaacg
    19561 cgccgaaaac gccggggtca gatccactgg ttcgctgtcg gaattgtgct cgagcgccga
    19621 tctaatcgtc tccatcgtag tgccaacggc ggcaatcaac gtagcagcag aaatcgccaa
    19681 ggccgccaca cacgcgaaac gcaagggctt gatttatttc gacgccaatg caatttcgcc
    19741 gatgactgcc gagacgatca gcggagttct cactgaaagc gccgtggagt tcgttgatgg
    19801 ctgcattatc ggatcagccg ccaaaatgga caaaggatcg gtcgtctacg cctccggtcc
    19861 ggcggcaacg cgcatcgccg tactcgggga ttacggccta agcgtgcgag ttcttggcac
    19921 tggaacgtcg caggcgtcgg ccttcaaagt agtctacgcg gggttgacga aaggattgca
    19981 aggcttattc gttgagctgc tgatgggggc caagcggttt ggtttgctgg atgagatcgt
    20041 caaccgatac gatgagagtt tcccgggctt acttgagaaa gtaagttcca gcatcgttgg
    20101 gctgcgcaac catgcggcgc gccgtgcgga ggagatggat gaactccagc gcacgtatgc
    20161 ccattacggt atgacggcgt taatggcccc ggcagttcaa aaggtccttg gcagcattgc
    20221 cgatttggct atccgcgaaa gctcggagaa aggcgcgcgt gcaggagatc tcatggagac
    20281 cctggagttg ttttatcaac agggcctttt gagcgatgcg cggcttgcgt caagtgcgag
    20341 caagtgatag gcgcatggat gaatttaagt agactacgtg atttaaacag gaggagacac
    20401 acagtggcga aatcggaacg cagagtagag aaaaagcaac cacaatacgg gtcggatgtt
    20461 gttgtcgact taatgaaagc tcatgatatc gagtacgcgg cgttcaaccc gggggcgacc
    20521 tttcgtggca ttcatgactc catcgtaaac tacggtggaa actacaagcc ggaagtgatc
    20581 ttttgctgcc atgaagaatt ttccgtggcg atcgctcatg gttatgccaa agcgaaaggc
    20641 aagccgatga tcgccatcgt ccacaacatg gtgggtttgc aacatgcgag tatggcaatc
    20701 ttcaacgcct ggatagaccg ggtgccgatc attgtcttgg gcggcaccgg tccaatgaac
    20761 accaaacggc gacggcctcg tatcgattgg attcacacgg cgctggtgca gggcaaccag
    20821 gtccgcgatt atgtgaaatg ggacgatcaa ccctatacgc ttgccgatgt tccggattcg
    20881 tttatccgcg gctatcggat cgccacgacg gaaccgatgg cgccggttta tattaattac
    20941 gacgccgaca ttcaggaaga gtctgtttcc gcgccggtcg agttgccgga tatcagccgt
    21001 tatgcgccgc cagctccggt tcaggccaat ctggcgcagc tgaaacgggc tgccgagcta
    21061 ttagtcaatg ctcaaatgcc tttgatcatt gcagatagct taggacgcaa cccacaaacg
    21121 gtgcccgcat tggtggagct cgccgaactc ctggcgattc cagtgatcga taaaggcgca
    21181 cgttttaatt tcccaaccac ccatccgttg gatgcgaccg atggggcaaa agatatcctg
    21241 tccaaggccg atgtcatttt ggctttggat gtagctgatt tgtatgggtc gcttacgacc
    21301 gtcagcaagc aaacgcgaga ctgcgtctac gtgacctcgc cgtcagtaaa gattatcact
    21361 atttcgatgc acgacatgct ggtgcgctcg tgggccgggg attaccaggc cttgcagccc
    21421 accgatattc cactaactgc cgatacttcg gtggcggtgc cggagttgac tcgcctctgc
    21481 cgcgaacttc taggcaatga ttcaaagagg aagtcgacaa tggaggcaag acagaaagaa
    21541 ctggccgaca agagcaagag tcggcgcgcg aagtggatgg ccgacgcgca agccaaggct
    21601 tcactgcaaa acatctcaac cgcttttctc gcgtatgatc taggtgaagt aatcaaaaaa
    21661 gaggactggg tgctggtgaa cggaacgtca aatggctggg cgcgccgttt atgggacttt
    21721 accaaaccaa accagtatct cggcgctagc ggcggggccg gagtcggtta tggcgcgagc
    21781 gccgcgatcg gcgcagcctt ggctctgaag ggaagcggca aagtcgcagt cgatattcaa
    21841 tcggatggtg atttgctgat gacttcgagt tgtctctgga ccgcggccaa acacaaaatt
    21901 ccgttgctga tagtgttgca caataatcag tcgttttaca attccgaaga gcatgggatc
    21961 gaagtggcga agtttcgcaa tcgacccgtc gagaacgccg ggataggaac tcacgtagac
    22021 gacccggaag ttgattatgc caaaatggca caatctttcg gcgtgcacgc cgaagggccg
    22081 atccgccgaa cctcggaatt gcgtccggcg attgagcgag cgttgaaata cgtaaaagag
    22141 aggcaactgc cagcgttcgt ggacgtggtt tcagagccgc gatagagttt ccgtagtgag
    22201 caatctgtat cttgccttcc gggaagccat tgattgcgcc gaagatcaga tcgatctcgg
    22261 ccgcgcagct ttagcgatcg ctacgcagga atatccggat cttaagatcg acgactgctt
    22321 gtcccagctt gatcagttgg gacaggcagt cgagcttaga atgggtgacg agaaaaaccc
    22381 ctatcgtatt atcgccgcgc tcaatacagt tctcttcaaa gaacaaggtt ttcaaggaaa
    22441 ccattctgaa tactacgacc cgaagaatag ctttttaaac gatgtcatca tccgcaagaa
    22501 aggcattcct attagtcttt cggtgattta catggaagtg gcgcgaaggg tcggattaac
    22561 gcttgaaggt gtaggcttcc cgggtcattt tctggtcaag taccacgaag gcgatatcga
    22621 aattctgatc gatgttttca acgatgggga aattcgtgct cgggaagatc tcgatcaaat
    22681 gctgcagcag ctttatcgag gtcaggtgtc gtatcaaccc ggttttgtcg ccactttagg
    22741 caaacgagac attttgcggc ggatgctcaa taacctgaaa tggatttacc tggagagagg
    22801 cgaaccgttg aaaaccttgt cggtgcttga tcagttggtg atactggatc cgagcgccgc
    22861 cagcgaaatt cgcgaccgcg ggctcttgta tatcaccctt gaatgttatg cgcaggcact
    22921 cgatgatctg gaaagctact taagattagc tcctggcgcc gaagatgcgc cgatgataaa
    22981 agcgcagatc gaatcggtaa gacatcgata cgtccagatt cagtgacacg caattctctg
    23041 caccggtttt ttatcgtctg gcgaattcct tgaagtttgc ggcgaaggaa gtcgttaacg
    23101 aaaaatcctg gatcatcgcc cgcgtaaggc gccatggccg gatgtgccat aatttcatct
    23161 ggcgcgcatt gtgaatcgta tggctccaaa tccgctgcag ttcctgacgg gaatagacca
    23221 cccgataatt gatcgcatca aacttaatca accgtaaact ggctcgcgag ggcaatgaac
    23281 cgcgtagccg tgtgtaagct tcctgccact cgttaacgcg atccgtcagg cgaacgttgg
    23341 cctgtaggtt gtgcatctca tcgaggcaac ttcgtgccgc atccgtttcg gcggaacgga
    23401 tctccggtat cttgtttcgt aggtgttccc attgcttgag aaagcggttt agatcgctgc
    23461 gattgtaaag aattctcgac gaaagctctt caagaggatc gagcaacgct ctggcccggc
    23521 ccgaaagcgt ttctcttgcg ctactgtaaa ctttttgtag ctcaccgatt cgtagagctt
    23581 gccagatgtc ggtttgaatt ttttggatct gctccaccca gcgctcctcg gtgtcgcttt
    23641 ttttcctcgt ttgcagccaa acttcttcca tttctttcaa gaacttggcc caattgacga
    23701 gcaggcgcga gatttcttta ctgcgcttcc acgcatgaac cgttatcgaa tcgatcgcgt
    23761 agccgggccg gcgcgctttg cgatccttga gccggaaaaa accggcgacc atcgggtgct
    23821 gtttttcgat taaagcggcg ttcttgtacc acaagaaaat cgacatcaaa ttccagtagt
    23881 ttctcggatt gtcggtccac cgggaaagga ccttgatcat attttctttg ctgtagaatg
    23941 ttctccatgc cttctcataa cactccgtcc actcttcttc ggtcatcagc ggatgcttga
    24001 tggtggcgtg aaatgagtcg cgcttgttga aatcggggtc catccaatca ccccgttttt
    24061 tcatctctcg atgatcgtgc gacccgggca ggggagtcag cataaaaaag gaagcctgat
    24121 cggggccgac ctcgtcgatg agaaagcgaa tgtcgttagg cacttgctgt tttgaatccc
    24181 acggcaggcc gatgatatag ccggtgtgaa ccacgactcc cacctgatgc cattcttgga
    24241 tgatctcgcg atactcttca actttgtttt gctttttgcc ttccgacgcc aagttttgcg
    24301 gattgacgct ttccatgccg ataaagacct gggtgcagcc cgctttcgca gcgagtgaaa
    24361 cgaaatcttt cggctttctt gccaggtcaa cttgcatcat gaaggtgacg tcgaggcctt
    24421 cttcacggag tttgataatc tcttcgaaag tttcgcgcca gagttttttg cgggcgaagt
    24481 tatcgtcagt gaaaaaataa aaagaaaccc catgatcaag gtagttgcgc cgcatcatgg
    24541 ccgcaatact ttgtgggctg cgttcccgca tcttacgtcc ttgaacgttg atgatcgtgc
    24601 agaagctgca agcgaacggg cagccgcgtg aggtgtctgc cgtgccaaag cttggataag
    24661 caaagtgctc catcgttttg gcgctggttt tcggcagcgg cgaatcctcg atgtcgacca
    24721 aactctgtaa gtcttgcgca aagctataga gcggtttgag tttaccttcg agaaaatccg
    24781 ctaggatcat gtcccacttg ccttcaactt cgccggaaac cgcacatatg ccttcacgaa
    24841 ataattcctg catgtcgggt tcctgttcgc ccaacatgtt gacggttccg ctggtatgaa
    24901 agcccccgat gatgactgtg atcccctcgg cgcgcagaac ccgcgccata tcgagggctc
    24961 ggggaaattg attggtttgc acgccaacca tacagaccag cagccgcgtg tgtggttcgc
    25021 tactccagcg aatgattttg tgcaaaggaa ctttttccgc ggtctcatca aatgtggtaa
    25081 cctcgattgg aagttctccg aacacacgac gctgtttaac atcttcagtc aagccatgga
    25141 gaacattcaa ggtattgctt ggcaggacgc ccttccagaa gcgaatgaca tagccatcat
    25201 cgtcatattt ggagggtttg atcaaaacta tccgaaattt ttcaaatgat ttttcctggt
    25261 ggatcacact gccctccgca agcttgtaac ttttgctgac atctcttata gataaattaa
    25321 taaaatcaag cctttgccaa ctgtaacaac caccctatac caagtcaagc tttttaccga
    25381 attggcgagc ttgtaacagc ttgccggcgc caccctcgat gattcaactg cttcatgtta
    25441 gcaaaactta tccgccgaat tcaccggctt tgatcgatat tacggtaagc atcgacaaag
    25501 gtcagttcgt gttcctcacc ggccctagcg gcgcgggaaa aaccacgctg cttaagctgc
    25561 tgttccgtga agaggaaccg agcgaaggac aaattatgat cgatcatcag aacgtcactc
    25621 gtttgcgcac tcgtggagtt gcccggctac ggaggcggat cggactcgtc tttcaagaat
    25681 tcagattgtt accgcggctc accgtaatgg aaaatgtcgc gctagcggcg caagtggtgg
    25741 gcgtttcgcg ccgcgaggct cagatcaaag ctgttcgctt gctccgggag ctcggattgg
    25801 aaaaaccaca agccagacca ttgatgctct cagccggcga gcagcaacgc gtcgccattg
    25861 cacgagctct gatcaatgaa cccagattga tattggctga cgagccaacc ggaaatctcg
    25921 atccggccct tgccgaggaa atcatgcgga tatttgttaa attgcgtgaa gctggaacca
    25981 ctctcatcgt cgccagtcat aatcttgatc tgattaaacg ctatggcagc agaattcttt
    26041 cactgcgtca gggtcgcctt gtcgatgatc tcgagagagt tatggaggcg cacgcgtcgt
    26101 gagctggtcg catttcggat ttgtcgctcg tcgtgtgcga catagcttat gggagctgct
    26161 gtggagccac atcctgactt cagggacaat ggcgatgtca ctattcgttt tcggtgtttt
    26221 tatcctagtt caagagaatc ttcagcatct gctaaacggc tggggagatc agattcaggt
    26281 caatacttat cttgagaata atgccggcac cgatcaagtc gaagcgatta tggttcgcat
    26341 tcgagcaatg cctgaggttg agagcttgcg ccatatctct aaagaacaag cctggaacga
    26401 tttccaaacc gctttagggg cgcagtcagc ggttttagaa ggcttgcctg aggacgtgct
    26461 gccggaatcg tttgaaatca cgctgaagcc agcatatcgg gacccgccgc aaatcgagaa
    26521 tttcgcgggt cgcctcggca aaatcaacgg aattgcggcc gtggaatacc cgcaacaatg
    26581 ggttgaccgg ctcaacctgt tcgtgctagc gctccaatgg gccaagtggg gattaggcgg
    26641 tatccttttc atcgcgacat tttttatcgt cggcagtacc gttcgcctgg ccttgttggc
    26701 gcgtaaggac gagatcgaaa tcatgcagtt cgtcggtgcg tccgaagaac tgatccgggc
    26761 gccgttcgtg gtcgaaggca tgctccaggg aaccgtcggc gcggggcttt ctctactcgg
    26821 gttatggcta ttttatgtat tattggagaa atacttggtc ggccaattag ctctgttcga
    26881 gcccaagatg cgcttgctat tcattgatcc cgccagcatc acgatgatac tcgttatcgg
    26941 cttgctgctc ggcgcgctcg gcagtctatt ttcgctcagg aggtttctta aaacgtggcg
    27001 cggctagccg ctatgggtgt attcctggct gccggtctag tagcggcgct cgcgcatgcg
    27061 gccagtgcca ggcgtgattt ggaaggcatc cagaagaaaa tcgaatcgga aaaacgtgtt
    27121 ctttcccagg tgaacaagaa ggaaggatcg gtgattcagt ccctaagtca ggtggagagc
    27181 gagcttgaac gaaaaaccaa gcaactcaag cagtcgacgg cgagactgca agtagtcgtg
    27241 gcggaaatgc agaagaaaga agcggaggcg ctgcgcatgc gtgaatcgct ggagcagcgc
    27301 aaaacctatt tggcccgtcg cgctgagtcg ctttatcggt ggcagcgaag tggcagtccg
    27361 ttggtgattt tcaatggtga tctatcgtta agctctttgc tcaaacgaaa atattatttg
    27421 cagcgcaccg tagcctttga ccgggagatg gtacaatcgt tgactgatca ggcggcccac
    27481 caggaaatgc tcgcgcaaga actggcccgg aaaagacaag acgttgacaa ccagagacgc
    27541 gacttagcag aggtcaagga agcagttcaa aaagaggcgc aaaaaaagcg tgagttgctc
    27601 gccagcgtta gagaggagaa agaatcgcgc cagcgcgcgt tgaaagattt agagcaggct
    27661 gccgtacgct tgcaaataat gatcgaggaa ctttccaggc gctcggcagc ccaatctaag
    27721 gaagctccgg ccgccggtgg tttggaaagc atgcgcggaa agctcgattg gccggttaaa
    27781 gggcaagtgc ttggcagctt cggtaaaacc aaacaccatg agtttgcaac cgaggtcttc
    27841 cgtaagggat tggaaatcga agctcccgtg ggggaggaga ttcgtgctgt tgaaaagggg
    27901 aaagtggtct tcgccgaccg attctccgga tatggcaaaa tgttgattat cgatcacggc
    27961 gatcgttatt acacggttta tgctcattta tcggattttc ttaaaaaaaa cggagattta
    28021 gttcggcgtg gcgaagccgt agcgctggtc ggcgatagcg attcactggc cggctcgaaa
    28081 ctttatttcg agatgcgcaa agacgggaaa tcgatcgacc cggcaccctg gtttcgaaga
    28141 taatagctat ttcaaaaccg ttgatatagt attgcttcac taaaaacatg ccagaactgg
    28201 cgaggtgaga tcctatgcgt tcgatgacca agcatggtgt gagtctttca attctactta
    28261 ttggaattgc gctgggcttc gttttagctg ggcaatgggt gccgaatgta tcggcggtgc
    28321 cccggcagga ctatgaaacc ttagaaacct ttaccaatat actgtccatc gtgaagaaga
    28381 attacgttga ggatgtcgat accaaaaatc ttgtcaacgg cgccatcaat ggcatgctga
    28441 cctcgctcga tcctcacagc gcctatctga cacccgacct ctataaagaa cttcagatgg
    28501 atacacaggg aagattcggc ggtcttggaa tcgagatcac tgttaaaaat ggtgtgctta
    28561 ccgtggtttc accgattgag gatacgcccg cgtttaaagc cggggtcaag cccggtgata
    28621 tgatcttcaa aattgaggat gattttacca aggacatgac actgatcgat gcggtcaaaa
    28681 aaatgcgtgg tccgaaaggc agcaaaatca atctttcgat caaacgggaa ggaattccgg
    28741 aactgatcaa tctaagcttg acccgcgata ccattcgggt tcaaagcgtt cgtagccgaa
    28801 ctttagagcc ggggtatggc tatatccgtc tggcgcagtt ccaagagcgc agtgatcgtg
    28861 atctccaaag ggcgttggag aaaatggcgg cggaaaaagg cggattgaag gggctcgttt
    28921 tagacctacg aaacaatccc ggcggtttac tgacgcaagc ggtacggatc tccgacattt
    28981 ttttggattc gggtttgatc gtttataccg atgggcgcat cgaatcccaa aagcagaaat
    29041 ttttcgccca gaaagacgct tcctcgatgg actttccgct ggtcgtactg gtaaacggtg
    29101 gtagtgccag cgcgtccgaa atcgtcgccg gtgctttgca ggatcacaag cgcgcggtgg
    29161 ttttggggac aaaaaccttt ggaaagggtt cggttcagac cattctgcct ttggacgata
    29221 actcggcgct acgacttact acggcgcgtt acttcactcc caacgggcga tctatacagg
    29281 ccactggaat cgttcccgat atcaccgttg agagcacgcc gcagcaggac gggaagctcg
    29341 acgagcgcaa gaggccgagc ctgcgagagg aaaacttgcc tggccatctg caaaacccgc
    29401 agcagaaccc ttccccgcaa gagcaactcg accaagagcg cgaaaaacag atcccgtctt
    29461 cggctccgac cggagatgaa accatcgaca atgacgctca actaaagcgc gctcttgatc
    29521 tactaaagag ctgggatgtg ttcaaacaag tcgtgcagaa aaaggccgca tagatttgtt
    29581 ggttatactg agtgtgaatt agccctctcc ggagggctaa tttgtttatg gaactagatc
    29641 gtgatcagga aagaagccga tttaccgcta tttacctcaa cgcctaaata tctcaccgtc
    29701 agtcagctca atcagctggt tcgggtgacc ttggaaactc gcttggatgc tgtttgggtg
    29761 cttggcgaga tatctaactt tcgtgtccct ccttcgggcc actgctactt cacgctcaag
    29821 gataacaaca gccagatctc ggcggtgctg tttcgacgac aaggtcagaa cctagcattt
    29881 gtgccggaga acggcatgca agttttatgt tttgcccgag tcagtcttta cacagcgcgc
    29941 ggagacttgc agatctatgt cgaatctctc gagcctcgag gccaaggcgc gctttacctc
    30001 gcgttcgagc aacttaaaaa aaaattggct caggaaggct tgtttgctgc cgagcgcaaa
    30061 cggcctttgc ctttcttacc cgataccatc ggaatcgtga cgtcggacac cggtgcggcg
    30121 ttgcacgaca tgctgagaat catccgggat cgttatgctg atcggcgcgt catcgtccgg
    30181 ccggtcaagg ttcaaggtag cggatcggct aaagagattg ccgccgccat ttcggatctt
    30241 tgtacgaccg gtgtccaggt tatgatagtc gggcggggtg gcggctccct ggaagatctc
    30301 tgggctttca acgaagaagt cgttgcgcgg gctatttttg cctgttcggt gccggtgatc
    30361 tccgccgtcg gtcatgaagt tgattttacg atcgccgact ttgtcgccga tcagcgcgcg
    30421 ccgacgccga ctgctgccgc ggaaatggtt gtgccgttta aacaggattt gttggcacag
    30481 ttgcaactga tcgagaacca attgcttcga tgtatgcaaa gtgaaataga caacgggcat
    30541 gaaatctgcc gcgggctggt caagagattg ctagatcccg ggcgcaagtt gcgcgaaaag
    30601 caacaacatc tcgatgatct ttcgttagat ctgcagcgcc gtttcagggg tcgactgaat
    30661 cagttcaaag aacagctcgg ccatgaaggc agccgcttga atgcgctgag tccactggct
    30721 gtcctagatc gcggctacag tatcacgcac aagatgcccg atgaagtaat cgtcaagagt
    30781 gctgattcgt tggacgtcgg tgacatggtt cggattaccc tggctcaggg caaagcggat
    30841 tgtcgtgttg aaggcaaaga gagatgagca tccttgtgct tctactatgc ttcctagatt
    30901 cggctatcta tgccgcggaa cccttcgcgc taagttggcc gtctcgactc gaaatcccgc
    30961 gaggtgaaat cgccgaattg aaggtttatg gcgaacggct atcgtcggtt gaaggacagt
    31021 ggggggcaga gaatcttccg ttctatgcag tcgcaccagg atcgtttgta gcgatgatcg
    31081 gtgtcgattt ggaagccaaa ccgggagtgt ctaaaatcac cctgaccgcg acgaacgatg
    31141 ccggcaggca gcgacaggcg gaaacgatcg tgcagacgct gtcaaagccc tttcggcagg
    31201 agtcgtttac cgtggctcca ggattcgatc agttaagccc gcaggcgctc gagctcattc
    31261 gtatcgagcg ggcagacttt gcgttggcat tctcgacgcc ggccgcccaa cgtctctggg
    31321 aagctccttt catccggccg gtgccccagg aatcctcttc gtcgtttggc tatcgccgta
    31381 tcatcaacgg aaaaccgcga gcgcctcata ccggagcgga tctgcgagct cccatcggta
    31441 ccgaagtcct ggctgccaat cacggccgag tcgtcctggt cggcaactat ttcttcgccg
    31501 gccaaagcat cgttctggat catggcggcg gcctctacac gatgtatttt catttatccg
    31561 acttcaaagt tgatgcggga gcgttggtgc gccgtggcga tgtgattgct ttgtcgggga
    31621 tgagcggacg agtgacgggt ccgcacttac attgggctgc gcggttgggg aatgctcggg
    31681 tcgaccccat ggaattgatc cagaagattt cgagcgggcc atcgcaacaa ccaggaagcg
    31741 ccacgggaag ttaaatcaga tggagcatgg tgtggtaaaa aaagaatcgc aagaattgaa
    31801 attcgaagag gccatcgcaa atctagagca ggttgtcgag caattggaat ccggcgacct
    31861 ttcgctggag gattcactgg cggttttcga aaagggcgtg ggactggtgc gctactgtaa
    31921 tcaaaggttg agcgaagtcg aaaaaaaagt ggagttgctg gtaaaggaca aagacggcca
    31981 attgcagctc aaaccgttcg ggaacccggc ggcggattcg gaatcagacc agtgattccg
    32041 atcgcttcaa ccattacaga agcacctcga tacccagaat tctaagctga cagctgcctt
    32101 actttctctt cacagcagcg gatcaactca ttgtcggacg caaagctttc acgcttgagc
    32161 ggctcgctaa accgcacatg agccttccaa gttttacccg agctgtagtg tagaccggta
    32221 gggataatcg gcagtgatat gccgaggcga cgctctgcgg cgcatgcgat ggcgaagaat
    32281 ccgcgcttga acggtaaaaa gccgtgttta tccatctttg gcgtataaga tggatcgatg
    32341 ttcggatatc cttcaggaaa gatcacgagt aagcggtgat ccacgagcag ctgcaccgat
    32401 tctcgaaacg cccgacgctg atatcgctgc gtatcgtcaa gtgaaaagga aggtgaccgg
    32461 ccgcggtcat ttttatgttg caacgcgtcg gctctgagaa ccacaggcca tcgcgccagg
    32521 cgcgttagct gtgtcatcac cgaatgaata gccgcgtttt gagtccaatc caaggctacc
    32581 acaatgtgga attgacgcgg cagggcggcg aagagcgcca aaccatcgta aaaatgatga
    32641 tagtgtcgga cgacgataat cgcaggtccg gtggcgggga tgtggtcgag acctgaagag
    32701 atcgtctcaa gacggccgaa tgcgacggca cgggcgataa tccgcatcga ctttgctgtt
    32761 gcaatctcgg ctaaagatcc ggagtgggag gtcatgaatc tctgcgacag gaaatgttag
    32821 caggttccct ctggctctaa gattcccgcg cttctgcgga cataagaacg gccacgccag
    32881 ctgagttttc ttgagagtgc actttgaatg agccgaacgg cgacggcaaa gtcacagacc
    32941 ggcgataacc agtatgtcca gggccgccga ttataggctc tagcggtgcc gaacaaaact
    33001 cccgttctga agattaacag cagcgcattt aaggcgagaa acaatttcga cgctcctgcg
    33061 ttcggagcga aaatcaacat cggcagcggc aggccttgaa caaaaaacac ttcaagtagt
    33121 ccgatggtct gccgccaacc gaagtactga tcgcgcatcg gcagcgacct tggccaattc
    33181 tcccatgctt cacgccaatt gccgtacata aatacttccg ccagcccatg ggtttcgtag
    33241 aagcctaccg cctcgccgcg ctcgactaat cgacgaacga tcgtaatatc ctcacaaaga
    33301 gatgaccgag cggctgaaaa agcttgtgcc ttcaatagag tctccctggg gctaaaaaaa
    33361 cactgtccat tggcttgtac ttgatcgata tttcgataag cgcggccggg ttgtccgaaa
    33421 cggtaaacta aagtcgtcag caacgaaggg tgaaccagag cttccgatgg cccggataag
    33481 tgctgacggg tggctaccga aaatgtcgag actcctgtgc tcgccgcatg gcttatcaag
    33541 gagcgagaga gtttagcaga gacgcgcacg tctgcatcaa tacaaagaat ccatcgattt
    33601 gccggattcg agcgagcgag accgacctga agattccagg ctttgcctgt ccactgaggg
    33661 tcagcggggc cggcatcgat ccatcgcacg cgatcatcgc gcgactgata aatctcagca
    33721 atggctcggg tcccatcgag ggatccgcca tcaattacta agatctcggc cacttcattg
    33781 ggttgagcga tgagggattc caggcaattt gcgatgcgcg ttgcttcgtt gaggactggt
    33841 aggagtatag tgatgcgcgc cgatgagggc tggtcggatg tttcaacaag cctgccttgc
    33901 gaagttttcg ccatgcgaaa aaatactcgc gctgcaagta tcgtctgcaa taagccaagc
    33961 aacaaaaaca attgagttcc tactaatctt tctgcgtcga ctggcggggc gagccccgca
    34021 aggtttcgca aatactgtcc tgtgggatag cgatgatcgt aaaccggctt tgtgcgggat
    34081 cgggaacgat ttccggagag cggttcaaga accccggcca agcgccaagg actcggtgtt
    34141 caaagctgcg gacctcaata ctgccctcga ctcttcctcg ggtggtgatt cggccattga
    34201 gaatcgagtg acctcgttgg acgatataca actcgccgtc gggcagtagc ggcacggata
    34261 tcttcagggt ggcacccgga tgcccatcac catcccaatc gtaaaccgcc ggatcgtcta
    34321 attttgtcgg gagattcgct tcgctcgaac tcggcttgta gccgattcgt tcgactccta
    34381 gatccgctcg atagcgccat ccttgaccgt ccttctcaac ttggatggga taggtgtgct
    34441 tggccaatgc ggcaacgaac ttgtcgggaa ataccatctt gatcatcgcg gaatcttgga
    34501 gcacatggaa gtcgcatact tcgtgacgct gcagtaaccc agtgcgatcg cttctaacct
    34561 ctattaatga aaccgacctg gaagcggatt tggacatgcc gaaaaacggc aagcgtgtca
    34621 cggatgccag aatcatttcc agacgataca tgccgggtgg taggggattg tcgtcttgtg
    34681 ccgcggaaac cccgctcaaa aacatcaagc tgcttagtaa caccaagcat cggccaatga
    34741 accgtcgggc tgttttcatc ctgtcttttg gagccgtgtt cgtaacgctg tcctgcccca
    34801 gaggagtcat ttcgacttgc agccgatcga aattcgatgc gctaacctgt cgattcgttc
    34861 aggtggatgt tcctttaata aacagatgag tcagattgaa gtcccgaccc gaacgacagc
    34921 ccaattccac ggacatggtt attgaggaac attgcgcgct taaattgaaa cttacaaacc
    34981 gttaccgagt cttttttaat cccgcggatt aaagatcgtt ttcaaagcgc tgtgacggta
    35041 tttgaagatt cgatccagat ccggcgcgac aaaaaatcga tttaccaagg tgccgccgaa
    35101 aactgagtat tgaacgttat cgttaacgag agtgccgccc tggtgctcaa aaaaactatg
    35161 ttcatgctcc caaaaaagat aagggccttt agtctgccgg tcgataaatc cgtgcggcgg
    35221 attccaagcg ctgatctcac tttgccattt aatcggcacg ccgtgaatgc gcaagcggta
    35281 ctccagccgt gctcctgctc gcattattgg cgggacttcg gcaatgatct cgaaccggag
    35341 ccagccgggc gtgagccgtt caagattact cgggtcggcg aaaaacttaa aaatctcaga
    35401 gcgaggttga ggcagccaca gctcggttct taggtgatag attttcatgc cgttctcaag
    35461 gcagggtcga tcaaacgtcg tcgtggcgcg ggcaaatcga cggtagactc tcgaatcgca
    35521 acggttgaat ctcactctgg ctttctcaac gccgcgacct ttttttcctc ccggtttagg
    35581 tctatttacc tcgaatgttt cttaacggca gacctccatg ttgactatca aatacattta
    35641 tcaattcaaa gtcaactata cttgacttta taggcaagaa atgttgagag taatcgagat
    35701 gaaaattgta atcacgggag cctccggctt catcggatct cgtctggccg atcatctctg
    35761 gaggcagcgt catcagctca ttttactcag ccgccaccct cctcgggagt taaatgtcac
    35821 tcagcacgag tgggtttcat ggattcccgg cgtatccggt gaatgggtag aggccatcga
    35881 tggtgtggat ggcgtaatca atctcgcagg agaacctatc gcggccaaaa gatggagcga
    35941 tgtgcagaag gagaaaatca ggtttagccg ggttgagacc acgaaggctt tggttaacgc
    36001 aatcgccaac accaaacaga aaccaaaatt tctcatcaac tcctctgcgg taggttatta
    36061 cggtggccgc ggtgatgaaa tcgttaccga agaaactgcc ccgggcagtg attatttagc
    36121 gcgagtctgt gttgattggg aaaaagaggc cagaaaggcc gaatcgtttg gtactcgggt
    36181 cgcgctggtt agaacaggga tcgtcttgga taaaggccag ggagcgctgg caaaaatggt
    36241 cgtgccgttt aaatattttg tcggcggccc cctggggtcc ggtcatcaat ggatgccctg
    36301 gattcatatt gaagaccaag ttggcttact gttgtttcta atagaaaacc aaaacgctcg
    36361 gggccctttc aacgcgtcgg ctccaaatcc ggtaaccatg acggaatttt gcaaaacaat
    36421 cggcaccgtt ctcaaccgac cctcgtgggc gactgtcccc gctggcatat taactctggc
    36481 gatcggcgag atggccgaga tgctgctgaa cggccaacgt gccgtgcctc aggctgccgc
    36541 gaagctcggt tttgaattca agtatggcaa tctgttgccg gctctggagt ccctgcatct
    36601 atgaaacgct gatgagcgcg ataaacgatt atcaaactgg ccaacgacag cgactttatg
    36661 ttcgggacct tctaaacggt gatgacgagg ctgcttttca ggtcatccat cggatgctgc
    36721 ttgcgcgccg ttcgctcggc gatgtttatt taagtttgat cactcccgcc ttgaccgaga
    36781 taggtcaact ttggtgtgat ggagagatcg gcgtggggct ggaaaaactt gcctctcatc
    36841 ttgtgcttaa acatatggac cggttgcgcg gcatgcacac gagcgagagg caacgattac
    36901 cgcaacgtat cttggttgca tgtgtagaag gggaacttca ttgcatcggc gcccggatgg
    36961 tggcggattt gttggacatt gaaggatggc cggtggactt tctcggagcc gacgttccca
    37021 cggcttcatt gatcgacata attaaaattc gccggccgca cttagtggca ctttcggtta
    37081 ggacatcgag cggtctcgat catgcccgcc atgtggtagg agaattcaag acgatggcgg
    37141 atgcgccaaa gattcttttg ggcggccagg cgatggcacg gaatgagtcg ccgtcagacg
    37201 caacctgcgc agttgctcgt aacgcccttg aaggagtgca gcttgctggc aagctgctga
    37261 acgcggggcg gcctaaagct gtgttgaaag aatacctgat cggattgggt cgccgagtgc
    37321 gcgacttgcg caacaaaaag ggctggaccc aagagcaatt agccgaatca gcgcgcctta
    37381 cgcgtgtttc catcgttgct atcgaaggcg gaaaacagaa catttcaatg gatgtgatac
    37441 tgcgtctggc gaattcattg gcgacttcgc cggaagatct actatccagt caccgtatct
    37501 tacggtctta gggggagcaa ttcttgaaga acttaagcct caatttgtcg aacgaggtgc
    37561 cggctggccg gattttcttg gagcaggcaa aaaatcttga gcatcggagt atagttcgag
    37621 aaggactcgg cagcatgctg ccggagcctg aacgcatgac gcaactgctc ggtttggatg
    37681 acccctcgat cgtcctggat gttcttcgta cggggctgct cgatcctctg gcagaagcga
    37741 cttcgacccg gggtaaacgc atacgaggac agctggtgac gttgggctac cggctcctca
    37801 ccgacactcc agcatcgctt cttgccgcca agcaatgcgg ctggtgcgcc gctgtagtcg
    37861 aaatgattca tacaggatcc ctggttattg acgatatcga ggatggcagc agcgtgcgcc
    37921 gcggccgtcc ggcgctgcat ctacaatttg gtatccccat cgcccttaat gcaggcaact
    37981 ggctctactt ttggcccttc gagttgcttc gggaaaccga actgcccgtg gatacgattc
    38041 gataccttta tgagcattgt catcgaactc tgctgcgcgc ccactttggg caggcgattg
    38101 atttaggcgc cccgcttagc tccttgcctc agcaccgcgt tgccccagtt tgcctgacat
    38161 ccatgaaact caagaccggc gcattgatgg gcttcgccag cattttgggc ggcgcgattg
    38221 cgcgagcatc agaaagtgtc ttgtcgattc tcgatcagtt cgggcgggat ctcggcgtct
    38281 cactgcagat gttcgatgat ttgggcaatc ttatcgggaa atgcgaacca gccaagcggt
    38341 atgaagatct tagtttatct cgaccgtcat gggtttgggc atgcgccgca gagagcagca
    38401 ccgccagagc gtatggagag ttctgtatcg cagtcggcag tttaccggat acgcataaac
    38461 ttgagtcatg gtccgaagcg cataaccttg tagaacgcgt ccgtgcctgt gcccatggat
    38521 atttggatga cgcgtttcgg cagctaaaag agcgccttga cagcgccaac gtacgctggt
    38581 cgaagcgtgc tttcgaagat ctgcgagaac tcggtgagga gatcacggtt gcatatggat
    38641 aaagtctctg cggctatcat tggaagcggt ttcggcgggc tcgccctagc catccgcttg
    38701 caagcagccg gttgtcgcac ggtaattttt gaaaagcgcg accagccagg gggacgggcg
    38761 tatgtttatc gagaccaggg ctttacgttc gatgccggac cgacagtgat tacagctcct
    38821 gattgtttag aagagctttt taccttggca ggaagatcga tggctgatta tgtcacgttg
    38881 ctgccggtca aaccctttta tcgtttattt tggcaggacg gttttagttt cgattattcg
    38941 gccgacctgg ccgatgtcga gcggcagatc gccatcaaag caccggggga tctgcacggt
    39001 tacaagagtt ttcttaaata ttcggaaaag gttttcgaac aaggatatgt caagctggcg
    39061 cacgtaccat ttctggattg gtcgagtatg gcgaaggtag cgccgcaact gctccggctc
    39121 aaagcttatc gatcggtgta tagtattgtc gctaagttta ttcgagatcc gcatcttcga
    39181 cagcttttta gcttccatcc tctactcgtc ggcggcaatc cgtttaccac cacatcgatc
    39241 tatacgttga tccattgttt agaacggaaa tggggtgtgt ttttccccaa aggcggaacc
    39301 ggcgcactcg tgcagggaat gctgaagctg tttcaggaat tgggcggaag aattcgattg
    39361 cagtcggagg tgtccgagat tctaacccgc ggcaatcggg tagtggggat cacgaccaaa
    39421 accggcgaga cacacgagtg tgatttagta gccagtaacg ccgatgtggt tcatacctac
    39481 gatcgccttc tgcgtcgcga gccgaaagca cgccgcatgg gcaaaagatt ggccaagatg
    39541 tcttacagca tgtcgttgtt tctgatttac ttcggtactc gccgccaata tccaaacctg
    39601 caacaccaca acatcatctt tggaccgcgt taccgggagc ttctgaagga catcttcggt
    39661 cgcggccggt tggcagatga cttctcgctt tatctgcatg cgccaacccg atccgatcct
    39721 tcgctggcgc caccgggttg cgaagccttt tatgtgctat cacccgtgcc gcatttgggt
    39781 aagctgtcgg tggactggag cgtggaaggg ccgcgttatg ccgagcgaat cctggactat
    39841 cttgagcggc gttacactcc caacctgcgc aacgaaattg tcacgcagcg gatttttacg
    39901 ccccaggatt ttcattcgga gctgaatgcc catttggggt cggcgttttc actcgaaccg
    39961 cgcttgatgc agagcgcgta ctttcgggtt aagaatcgcg atgcaaatct cacgggtttg
    40021 tatctcgtcg gggccgggac gcatccggga gcaggaatcc ccggtgtggt aaactcggcc
    40081 aaagccactg ccgggcttat tatggatgac attaagaaag cggtggtaat gcgcagttcc
    40141 agccagacgc agagactatc ctatgcctga ggcgatgtcg cccaaagtca aagcggcatt
    40201 ggaagaatgc cgctggatga tcacgaaagg ttcgaaaagt ttttcgctcg cggcgcggct
    40261 ttttgactcc gacactaggg atgcggcatt ttttctttac ggctggtgtc gctattgtga
    40321 tgatcaggtg gaccgggcgg gaatggcgga gagccgcgaa caacttgaac ggcgcctagc
    40381 caagctctct gaggctaccc ggtccgtgtt ttccggcgct gcacaggagc aagcggtctt
    40441 tatcgcgatg cagtacatcg tccaacgtta ttcgattccc gcgcattatg cgctggaact
    40501 gatcgaagga atggcgatgg atgctcgcgg cacccgctac cagacgttca aggatctttc
    40561 gctttactgt taccgagtcg cgggaaccgt gggattgatg atgtctcatg tgatggggtt
    40621 gcgcgacgaa caggcgctca agcacgcagc agacctcgga attgcgatgc agcttaccaa
    40681 catcgcccgg gatattaccg aagacgcgtc gatgggtaga atctatctgc ctctggaatg
    40741 gttaaaagaa gcggatatag cgccggaaga gatcgccgcg gtaaaaaatc gaccaaagct
    40801 ggcgctggtc acgaagcggc tccttcaaga agcgcagcgg tattatcttt caggggatga
    40861 cggcttgtgg cacctatcgt ttcgctcggc gtgcgctgtg gcggcggcgc gccatgttta
    40921 tgccgagatc ggttcgcttc tcctggataa agggccctcg gcttgggacc agcgcagcta
    40981 tattacattc gcgcgcaaac tttgggtcat actctgcggg gtgctccacg tcatcgcttc
    41041 catgccggcg cgattgatgc ggccatggtc tgccgcgcca attcataagg tctggcggca
    41101 gtcggagctc cggtgatccg ggaaaaattc aattcatagc aggagattgc ccaatgcatt
    41161 atatcccctg gctgtttctg atcgttcaag gcgcaatcgt cgttgcgagt ttgctaggct
    41221 acggtatctt cacttctcgt cctgagcttc tgatacaaat cgatccgggc gcacgttttt
    41281 ttacctgggc tttttacgga tttgccgttg gcaatatgct cttcggcggt cttgcggtgg
    41341 tggcagaagc ggttttgcgt gaccggacga acgctctgtg ggcctttgcc tcgatctatt
    41401 tcgtcagtct cgccagcgaa ttgcttggca ctacctacgg aatcccattt ggcgcgtatt
    41461 cctataccgc gctgctcgga gttaagtggt tcgatcgcgt ccccctcttg attccgttga
    41521 gctggtttac aatgagttgg gcatgctggg taattgcgcg gcagcgcagc gccgcactgt
    41581 ctgccgtcgt tctcgccacg gctctcctgg tcgcatggga ttttttgctc gatccggcga
    41641 tgagcaaagt caccagttac tggatttggg gacaaagcgg cacctactac ggcatgccct
    41701 ggatgaactt gctcggctgg ggcattaccg ggttggtgct attcgtcata ctctgcaaaa
    41761 ttgcgccggc gccgcatacc gaacgcagat ttgcaatttg ggtatatatg atcaatctcg
    41821 ctttgccgct cggattttgt gtgttgaatc agtattggtt ggcggttttc acgggcgtgg
    41881 gcactattgc gattgcgctt attgtattcg gcaagaaggg gagcattttg gcagcgggcg
    41941 aaatggaaga tctctctcct tccggacggg cgggggcctt gtcttcgcag cgagaataac
    42001 ggcggatttg caattgaaga acgggtgttc tcagggtaaa ttgtaggata ttttgctttt
    42061 tatatcgtta gtcagcttaa ttgagctcgc tcaccgccta tgcgtccatg aggatcggcc
    42121 cggtgattcc gtctgaactg agcgattatc ttcaaaggca gcggaccgcg attgatcgtg
    42181 ctctcgagca gtttctgaaa gtctatgggg gacagaccca agcgattctg aaagccatgc
    42241 gttatgggct gtttcccggc ggtaaacgca ttcgcccgat cctcacatta acagccggag
    42301 aaatgttcgg cgctaaacag aaggagctta tgccgtttgc ctgcgccgtg gaattgattc
    42361 acgcttattc catcattcat gatgatttgc cggcaatgga taatgacgac gtgcgccgcg
    42421 gcgaacctac cgcccataaa gtcttcggcg aaggtatggc tttgctggcc ggagatggtt
    42481 tgctcaccga ggcctttcat ttgatctccg gtccagaagt caggcgattg cccgccgagt
    42541 tggttttgcg gctaatccac gaattgtcac acgccgctgg aatcgcaggc ttggtcggtg
    42601 gccaagcagc ggacttggaa tcggaaaaca aagatgtcga tcttgcaacg gtcgaatata
    42661 tccatgtccg caagaccggc gctctgattc tcgccgcgct tcgcaccggc gcccaaatcg
    42721 cgggtgcaaa gacgggtgac ctgcgaaagc tttccaaatt cggcgagtat ttaggattgg
    42781 cctttcaaat tgctgacgac gttctcgaca acatcggaga gacgctggct ggcgaggggg
    42841 ctgagtcggg tcgcaacgag ttaaagaagg cgacctatcc atccgtcgtg ggaattgttc
    42901 aagcgaccga acgcttgcac gagctgttaa gattgtgcaa ccgggaactg gtttcattcg
    42961 gagagagcgc ggagccgctt agggcgatcg caaaagacat catttcacgg gctgtccagg
    43021 accgagggag aacagcagag gagagtattt catagatggc taacttgctt gacggtgtcg
    43081 agtaccccaa agacattcgg agaatgacgc ttgatgaact caacgaactc gcagccgaaa
    43141 tacgtcaaga agtgatctcg gttgtgtcag aagtgggtgg acacttcgct tcgactctcg
    43201 gcgccgtgga gcttactctt gcgcttcatt acgtattcaa cacacccgaa gatcggatag
    43261 tttgggacac ggggcatcag gcgtatgcgc acaagctcct atgcgggcgc cgcaaccgtc
    43321 tgtcaacgat ccgccaattt ggcggcctca gcggtttttt gagccgggat gaaagcgagt
    43381 acgacgtttt tggcgccggc cacgccggta cttcaatttc ggcggcgcta gggatggtcg
    43441 aggcaaagac gctcgagtct tcttctcgca aggtggttgc ggtgatcagc gatggcggct
    43501 tgagcgccgg actgacttac gaaggcttaa accaggccgg gcatttggag aaggatctga
    43561 tcgtggttct caacgataac gccatcttta tcgatccacg ggtcggcgcg gtgtcatcct
    43621 ttctgagcaa gcaatttact accgatgttg ccctccggtt gcagaaaaat ctttccgccc
    43681 tgttacgaac attgcctaag ggtgaaaatc tgtttcatct ggcgcgcaag gttaaggagt
    43741 cttttctcgg tttagtgacg ccgggatttc tcttcgagag ccttggtttc cagtatgtcg
    43801 gtcccatcga tggtcacaat atcgaagaaa tgatcgcgac tctggaaaat gttaagaaga
    43861 tagagaaacc aactctagtc catgtcgtga ccactaaagg gaaaggctac ccgcctgctg
    43921 aaaaagaacc ggtaaaattt catggtgtca ctccttttca cgttcttacg ggaaaaccga
    43981 aaaaaggcaa aggtccgatt ccaacgtaca cggacatctt cggcgaagcg ttgatccggc
    44041 tggccaagga aaatcccaaa gttatcggca ttaccgcagc gatggggagc gggacaggaa
    44101 tcgataaact tgcgcgcgag cttccgaata agtcttacga cgtcggcatt gccgagcagc
    44161 atgccgttac ttttgcggcg ggcctggcga ccgagggatt cgtgccggtc gtggcgatct
    44221 attcaacctt tctgcaaaga ggatatgacg agatcttgca cgacgtctgc ttacaaaatt
    44281 tgcacgtcgt ctttgctctc gatcgcggcg gcctcgtcgg cgcggatggt ccgacgcatc
    44341 acggtgtttt cgattttgct tatatgcggt caattccaaa catcgtcatc atggcgccga
    44401 aggatgagaa tgagctgcaa catatgctaa aaaccgcggt cgactattca gggcctattt
    44461 cgattcgtta tccgagaggg gagggttgcg gcgttgaaat ggatgcgcag atgaaaaatc
    44521 tagaagtcgg cagggcggaa ttgctcaggc acggaaccga tgtggtgatc gccgccattg
    44581 gccagaccgt catgccggcg ttgcgagctg cccaggacct ggttcctcta ggaatcgacg
    44641 cggccgtggt caacgcccgg tttgtcaagc cgctcgatcg agagctgttc cgagatttgc
    44701 tgttgcgtgc tcctcgttta atcacggtag aagaccatac ggtggtcggt ggtttcggca
    44761 gcgctctctt ggaatttatg gctgaagaag ggatcaccgg ggtggaagtt aggcgattgg
    44821 gcgtgcccga tcgttttgtt acacacggga ctcaggacga gctacgcaag ctatgcggtt
    44881 tcgacaagga cgccattgct caatcggcgt tacagatgat ccgccgggca aaaaggaaaa
    44941 gtaaagaggg atgggaaaga gggagcgcct agaccttcta ttggtgcagc gcgaactcgt
    45001 ctcgagccgt gaagaagggc ggcgaaggat actcgcggct gaagtgctgg taaacgaaca
    45061 gctgtcgaca aaagcaggcg cgttggtcga tcaaaccgcc gaagtccggc ttaaagcagc
    45121 ttctgcttat gttagtcgtg gtggcttgaa gctggaaaag gctctgcgct ttttccagat
    45181 cgaagtgaaa gataaggtgg ctctggatgt cggcgcatca accggcgggt ttacccattg
    45241 cctgctctct catggcgtgg cgcgagtata ctccgtcgac tgcggctatg gccaactcga
    45301 ttggaagctt aggaacgatc cacgagtagt cgtcttagag cgaagaaaca tacgttatct
    45361 tgaagcttcc gatttgccga gccttgcaca cttcgcgacc atcgatgtct cgttcatctc
    45421 attacgtttg gttcttccca aggtaaagac tctgatatcg cccggaggcg agatcatcgc
    45481 gctaatcaaa cctcagtttg aggttggcaa aggcaaggtc ggcaaaggtg gcgtcgtccg
    45541 ttctgatgca gaacacttgc gtgtgatcga tgaaatcgag cagaccgcgg caggcttggg
    45601 cttcacaacc cgaaacatca ccgaatcacc actgctcggc gccaaaggca ataaggagtt
    45661 cctcattcat ttgactgatc cgggtttccg gtcctaattg cagatattgc gccggtatca
    45721 attcgcgcgt tgtctgttaa tctgtgctgt ctatggcaaa gtttctgcgg atcgcccatc
    45781 gcggcgcgtc tgggagcttc ccggaaaaca cccaactagc ttttgaaaaa gcgattgaag
    45841 ccggtgcgga tatgatcgaa atggattgcc aattgtccca tgacggtcat gtggtcattt
    45901 ttcacgatga gcggctggtt cggattacag gtgctggagg catcgtcaat cgcatgcctt
    45961 tagaacagct caagagattg gatgccggag tcttgcgaaa atccgcgttc aagggggaac
    46021 gcattccaac tctggaggaa gtcgtcgcgc tcgttggcgg tgaggctgac ctttgcctcg
    46081 atatcaaaca atacccgggt tctccgcccg gcattgaact caagcttctt ttcattctta
    46141 gccactacga ttatctcgat cgtacgatat tttcctcctt cgactatgcc tgccttcgca
    46201 gggtgcgcga gctggcacca gaagcccgac tgggcgtgat ttatggtaat ggcattgaag
    46261 agaatccatt cgcagtagct gcagaaatcg aggctgtttc tttgcacgtg caaaaagaac
    46321 ttgcgacccg cgagttcttg caaaacagtt gggacttggg actggatgct tttgtctgga
    46381 cggtcaacga agtacgagag atggaagatt tcgcatcatt aggtgtgcag ggaatcatga
    46441 ctgattttcc ggagcgcttc cggcatatgg agaaaacata aagcacatcg ctgctgttcc
    46501 caatcgccga gcaccttgaa gaatccgtta aatgcggccg ccagtcgcct acgaaaagcc
    46561 acaaagaagc ccgccgagtt gcgccctagc acgtagtgat tggcgtgaat tgcttgactt
    46621 gagatgtatt taggataaac ttgcattccc atgtgccttc agagatcggg ggtgatttta
    46681 atccatgacc ggtgttcgag ttagagaaaa tgagtcgatt gaaagtgcca tcaggcgttt
    46741 caaaaagctt tgcgagaaag ccggaattct agcggagttg agaaaacggg aacactacga
    46801 aaagcccagt gtcaaacgga agaagaaagc catcgcggct aaaaaacgcg cgttgcggcg
    46861 tgtgcgtcat tctttttgat gatctgaagg tttgcgcgtt ggtgtttaag ccaggaagat
    46921 attcaccatg cacttaaagg ccgaaattca ggattcgatg aagtcggcca tgaggagcgg
    46981 cgataaaatc acgctcgaaa gtttaaggct ggcactggca gctataaaga acgaggagat
    47041 taaatttcgg cgagagctca caacagagga aatccaacgg acgattgcga cgctctgcaa
    47101 gcagcggtcg gagtccattg agatgtttca aaagggcggt cgcagcgatc tagtcgaaaa
    47161 agaacaggcg gagttgaaag ttcttcaacg tttcctacct gaacctttga gcgacgacga
    47221 aatcaaatcg gcgattcgga gcacgatccg ggaatcgggc gcacagggcc tccaagatct
    47281 tggcaaagtg atgaaagaag tgatgccaaa ggtaagcggc cgaagcgatg gcaaacgagt
    47341 caatgagctg gcccgagaaa tacttcgtgg cgcctgaatg cttatagcgc acggtctctt
    47401 taccttccaa atcaaacaat tcatgaccgt tccttccctg cttaatgggg ctgagcagtt
    47461 ttcaccagaa gaatgtgacc cctgctcgta atgcattcga accaaccatg tttcaccggt
    47521 ggtggtattt aggatccgag agagttatca agacggatgg ctgtcattgg tgaacacctt
    47581 cgatatcatc ctgctatgtt ttctttttct gtttgcgttg cgaggctttc tcaaagggtt
    47641 tctccgagag atactttctc tcgccggtct gatagttggc ttcatgatag cagcgcgtta
    47701 taacgatgcg gtagccatgc tcggccaaga gtactgggaa acatctctat ttgtcctcaa
    47761 gggagcttct tttgtcgcaa tcttttttgc cgtgtatttg tgctttaacc tgttgggctg
    47821 gttttttcat cgttccgaga gatttctttt tctgcaaaca ttgaatcgag cgggcggtat
    47881 tgttattgga atcggaaagg gtgcggcact ggtggcttta atcgtttact ttatgagctc
    47941 cgcgtcttgg attcctgctt ctgcgagtga aaagatcaaa acttctgttt tgaccccacc
    48001 gttatcccga ttagccgaag tgatcattcg agtcggaaag gaaagaatct taacgacagt
    48061 ggacaatcgg gtgcgactgt tcagtccaac tcgtgtccta taaattgccg catgatcact
    48121 caggagaaga tcgctgagat tcgtgctcgg gcctcggtcg ttgaggtcgt ttccgattac
    48181 gtcacgctaa aaaaggttgg ccgtaatcac gtcgggctat gccctttcca aggcgagaaa
    48241 actccctctt tcacggtgaa cgatgaaaag ggtatttatc actgcttcag ctgtaaggcc
    48301 ggtggcagcg tttttaactt tttgatgcaa cacgatcatc tctcgtttcc tgaagccgtg
    48361 gaatgtgtgg gtaagcgcta cggcattgcg gtaaagcatc taggcaggaa cagcgggtca
    48421 ggagaagagg ccgatcgcga ggcgctctat cggctcaatg aagctgccgc tgccaattat
    48481 catcgagtcc tgttcaaaga cgttttggga aaaagagctc tcgaatacat aaaaaaacgc
    48541 ggcatagcgg agccgaccgc gcggcgtttc atgttagggt ttgcgccacc ctatggttca
    48601 gggcttctcg atgtcatcaa gaaggaaaag ctttccctga aagatgcgct taggctcggg
    48661 ctgattggcc aacgggaagg caatcggttc cacgagaaat tctttgaccg gctcatcttt
    48721 cccatcgtta acgccggcgg caaagtcatc ggcttcggcg ggcgggtgct ggagcaagga
    48781 attccaaaat acctcaattc aagcgatacc ccgctttttc ggaaaggatc gaccctgtac
    48841 ggtcttttcc aggccaaaga agcgattcgc aaagtagacc gggctgtcgt cgtagagggt
    48901 tacctcgatg ttatcgcttt gcaccaatat ggcgttgatt ataccgtagc tactctcgga
    48961 acagcgctta cagtggagca cgtccgcggt ttgtcgcgct acacaaaaaa cgtcgtagcg
    49021 tttttcgacg gcgatgatgc cggtagtcga gccgcggagc gcagctttga aatttttgtt
    49081 gaagccggac tgcttgggcg agccgctttt ttgcccaacg gtgaagatcc ggatactttc
    49141 atccgcagtc gcggcaaaga agccgcagaa gaactgctcg accgatcagt gccactggcg
    49201 gactactact tctcgcgcct agagcagcgc catgggaaaa ctttagaagg caaaagccgg
    49261 attgccgagg agatcgcgcg cctgctgcaa aaggtgcgca acccgttcga agtcgatctt
    49321 ctaattgcga gggctgtaga ttcgttaggt atccgcgagg agttctttcg caagcctcta
    49381 cagagtgcgg ttcctgccaa gacgccctca aatgccatcc aacggctttc tgcgagcgac
    49441 cgcagcgatc gcggcgatgt agcggaaagg tcgctgataa ctctgatttt acgctatccc
    49501 gtaatcgccg gatatgtatt ccgagacaca gacgtgcgac aatggctcgg atcagattgg
    49561 cgggaaattc tcgatctgat cagagccgat tggcaagagc atggaaacgt cgacgtcgca
    49621 aggatcctgc aggccttcga atcatccacg gcagccgcca ttgccggtct tgcaatcgaa
    49681 ggagaacagg ttccagaagc tgagtctcaa aaaatggcgg atgactgcat cgctcatctg
    49741 cgccgaaagt ttatgcgaga gcaagaaaga aatttgcgaa tcgcgatccg aactgcggaa
    49801 gaacagaagg acgagaacgc aaccagggag aggatactag aatggcaaga aatcgtacga
    49861 aaagaacgcc agctagaacg ccgaaagctc ggggcgaaga taaccgctcg ataggcaacg
    49921 ataaagccgc ctcacgtcat gctgccgggc aaccggcgga gaaaaaaaac atgaaggagg
    49981 tgaagaagct catcgatctc ggaaaagaaa agggctacct gacgtacgat gatgtcaacg
    50041 atatgcttcc tgcggaagtc gtttctccgg atcaaatcga cgatgtgatg tcaatctttg
    50101 gcgagatgga catcgaggtt gttgattcca atcaaagagt cacccttggg ggagccggtg
    50161 aagatcttgc cgatgatgag gaggatgaga aggagatcga aggcgacacg gacggggacg
    50221 cggtcggcaa gactggcgat ccagtgcgta tgtatctgag ggagatggga acggtttcac
    50281 tgctgagccg ggagggtgaa gtcgagatcg caaagaaaat cgaagtaggc gaaaagaagg
    50341 tcattgagga agtgttatcg agcccgcttg cattgcgcta cgttttggaa ctgggtgaaa
    50401 aactctcgca acacgagatc cgtgtgcgtg agatcatcaa agatggtgat gaggatgccg
    50461 aatatctaga agatgaagag gtgcatgaac gacgcctgct cgatcaaatc gctaaaatcc
    50521 gcagagccgc gaacgagcga gacaagatca aaaatgaact cgagggcaag cgcctgtccg
    50581 cgcaacgccg ccaggcgctg caacagactt tggaaaagta tcaggaacga attgtcggcg
    50641 gtctgaaaaa tctacagttg aatcgaaaac aaaccgaagc gatcgtcgac ggtctcaagc
    50701 gagcaatgga tcgtatgcaa actttagagc ggcgcgtaaa ggaattcgag caaaggaccg
    50761 gtaagacggc ctctgaactc ttaaagcttg ttgcccacct aaacggacgt aagaaggatt
    50821 ggcaacgcct caccagttca ctgcgaatgg gccacgatgc ggtcttgcac atgtcagacg
    50881 agatcaagaa cgcccggcgc gacatacggc atatcgaaaa agatctggac atcccggccg
    50941 aagaaatcaa acgccgtgtc tggtcgattg ttgacggcga gaccaaagcc aatctggcga
    51001 agaaagaact gatcgaagca aatcttcgtt tggtcgtgag cattgccaag aagtacacca
    51061 atcgcggact gcagtttctc gatcttatcc aagagggaaa tatcggttta atgaaagcgg
    51121 tcgataaatt cgaatatcag cgagggtaca agttctccac ctacgccacc tggtggatcc
    51181 gtcaagcaat aaccagggcg atcgccgatc aggcgcggac aattcgcatt ccggttcata
    51241 tgatcgaaac gatcaataag ctgatccgaa cctctcggta tctagttcaa gaaatcggcc
    51301 gtgagccgac acccgaggaa atcgccacga aaatggaaat tcccttggac aaggtaagaa
    51361 aggttctgaa aatcgccaag gagccgatat ccctggagac cccggtgggc gaggaagaag
    51421 acagtcatct cggagatttc atcgaggaca aaagaattat cactccttcg gatgcggtgg
    51481 tgaatatcaa cctgcaagaa caaaccaaaa aagtactggc cactttaacc ccgcgtgaag
    51541 aacaggttct gcggatgcgt ttcggaatcg gcgagaaatc cgatcacacc cttgaagaag
    51601 tcggtcaaaa gtttaccgtc acccgcgagc gcattcgaca gatcgaagcc aaggcgctgc
    51661 ggaaactaag acatcccagc cggagcaaaa ggctgaaaag cttcatggaa acctaagcga
    51721 gcgagtttct ccatcccgca caactcctta ttacgggccc atagctcagt tggttagagc
    51781 caccggctca taaccggttg gtccctggtt cgagtccagg tgggcccacc aatcttgatg
    51841 gtataaagaa ccgacactca gatgctgcca gaggtaactt gtagatgagg gtgagattgc
    51901 cgccacgcac tctcacgtcc ctcaacaatc cttgcaggaa tcccttttgc cccgattgct
    51961 tctccgctaa gcgacgccac atttccgcga ggtaagcgtc c
//
LOCUS       DASUVS010000370         8014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1687540, whole genome shotgun sequence.
ACCESSION   DASUVS010000370 DASUVS010000000
VERSION     DASUVS010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8014
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687540"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VF182_19885"
     CDS             <1..647
                     /locus_tag="VF182_19885"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6439385.1"
                     /translation="PGLAFEDLPPIHVVILSHDHYDHLDFATVKRLADTHNPLFLVPL
                     GMKAWFANDGMNHVEELDWWQEREYRGVKFVCLPAQHFSQRTLWDGNERLWASWAMLS
                     PGRRLYFSGDTGYFDGFKEIGHKLGPFDLAAVAIGAYVPPEIMKSVHTTPEEAVQASV
                     DLNARILLGIHWGTFDLAEEPLEEPPQRMLAEVRRRGIDEDRAWIFKIGETRKW"
     gene            complement(757..1554)
                     /locus_tag="VF182_19890"
     CDS             complement(757..1554)
                     /locus_tag="VF182_19890"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6439386.1"
                     /translation="MSPAKLLQSVLLLIATTTALPQTVLAFCHEMEVVQRPILRVATT
                     NSRPVEIEWFGHSTFQITSSKGTRILADPHSRSDLRWPSLDQHVVTTSHQHGAHNNID
                     MAKGNPMIFQGLTPTGENWNPVHETIRDVSVYSVPAYHDKSQGLQRGKNAIFVFRVDD
                     ICIAHLGDLGHLLTPPQLKLLGKIDVLLVPVAGGMFTVTPSEAIEVTKQVNPRIAIPE
                     HYWWAGAADQFISGFPRVKRSPAAILRVSKPELPQPVEIVVLSEGGR"
     gene            complement(1604..3862)
                     /locus_tag="VF182_19895"
     CDS             complement(1604..3862)
                     /locus_tag="VF182_19895"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX6439387.1"
                     /translation="MMKDLYLTSSVPWWLVALVVAACLALLVQQFLSLRRRLSIGQSI
                     FLVSLRAGVYGLLIFFLLSPALVEKQVRQLRRPLTLMIDDSQSMTFPANPGSAPDKAS
                     SRLDLVKQKLTDGEEPLIQKLSREYDLRIYRFGTSLEPIAPASVPQLKAQDQGTQLLE
                     LLQIAAKEAGPQSGIIIFSDGIANGDRQNLEGSSALPVPVFTVGAGDTEGFTDVRVTD
                     LRTPEFAFRGREFKFDLTVRASGMKGKTVPLYFNRGSGLITSRTVTIDDDDFTRKITL
                     SFTPKDIGPHHFSLNIPAQPGEQISQNNHKDFKVDVQRDKIRVLTLSGSPAWNYRFLR
                     MAMKQDPLIELVSFVFLRTPTDSVDVPDSQLSLIPFPIDDIFLEELKNFDVVFFDDFS
                     HRAYFNPVYLDKVKDFVRDGGGLAMLGGVRSFDSGGYGESALKEVLPVEMDGKGTYRN
                     DIAVPPALTASGKAHPITRLFPDPRANESAWAKIPPLTSINQVRAARGQTLLVTGGDG
                     AAKGSPLLTVGRFGKGRTLALMSDDIWRWNFIAVGNRESPQNHLKLIRQSVRWLAQEP
                     SFEQVKIQPIPLARPGEKLAIKLRVLKDDFTPTRQAAVQVRVFGPEGEPSLIAAKADS
                     AEGEYTGEYTPTREGSYRVEAEANLGGKPLGKDRSSFSTAFPYGETDDGRPRTELLQA
                     IAESSQGFYFPIADWNEKAVEQIAAKLEQHAPSQIVERRQTQLWSTLWTFLPILLLLC
                     VEWWMRRKWGLL"
     gene            complement(3862..4632)
                     /locus_tag="VF182_19900"
     CDS             complement(3862..4632)
                     /locus_tag="VF182_19900"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX6439388.1"
                     /translation="MNRRDFLCVLSKTLVTAGLLPAVAGASHPKGKTPTQFVFAQIKY
                     RGGDWNPHPLSVSPLMEELMQRTSVEAAPARHEVNLTDRDLFNYPFLYITGRYEFEPF
                     SAEEIETLRLFLSFGGFLLIDDGLGQQGYGFDKSIRREMQRVFPGNEFKRLPNGHAAL
                     RSYYLLRRIGGVRIVSPTLDGVTVGSSTPVVYCQNNLGGAWERDQLGKWTNACTPGGE
                     EQRRDAFHLGINLILYAMTENYKEDLIHVPFIRKRLTR"
     gene            complement(4661..6754)
                     /locus_tag="VF182_19905"
     CDS             complement(4661..6754)
                     /locus_tag="VF182_19905"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX6439389.1"
                     /translation="MSVFFLYPIFLFGLVAASLPLLIHLLNRRKLNRIRFPAVRFILM
                     SQKRISRSYRLRHWILLALRTMAVVLLALLLANPIFQTGAGLFAGGGPVSLVILLDNS
                     LSMTWSGDGIGFKQAKEAARLLITSLGDSDRAAIVPTSISGKEPIRLKVEKDVLLKEL
                     DQIEIADGTANFSTALAKAYELLGEPAGQKEIRLITDLGLTGWDNFSMSSLKQVDPAI
                     PVKAIRVGRKQKPLNVAVKEIRAAGQGIGVDLPLHLEAVVANFSDQEIKELLVQLSID
                     GQNKDQKLTSVPPRGDATITLQARLSQAGSHTGQLTIKKDGLAGNASMHFGVHAQDQL
                     RVLVVDGDPQTSLVQSETFFLTRALNPAGESDSSQYLPTVIIPEALSGAALDSYQVIV
                     LCNVASLPDSFIAKLQNYLRAGGGLLIFGGDRLQADHYNAKLAQILQTPIRDKKQGLE
                     ASGEKIGKMELTHPALQSLSDNLLQESIKSARIWGYSRISASGKSVLMSLANGDPLLI
                     EQKVGSGRVIFITTTADRDWSDLPVKTAYLPMIQSLTNYLAGGKRGLLDGGISVGDVK
                     QISLPPGYVGKTIGITKPNKQPAEVVMNAAQDRASGSFEDNDVAGIYQLALKDGDQES
                     GTPRLYAVNPPFLESRLEEISERELQAKLSPVRADVVPIESLRQGGIRRDLALPLLGL
                     LLATLLLEGWFAQRI"
     gene            complement(6761..7654)
                     /locus_tag="VF182_19910"
     CDS             complement(6761..7654)
                     /locus_tag="VF182_19910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX6439390.1"
                     /translation="MIANGQKKYFDPKVLAGISNLSLRARWVVEGIMSGIHRSRSKGF
                     SVEFEEHREYSPGDEIRRIDWKALGKFDRYFIKEYEDETNLRAYLLLDTSASMDYSSD
                     GITKFDYGCTLTASLAYLILRQQDAAGLVTFSNRIENIIPPRAKRDYLTQIVNALENQ
                     RPGGETNVGRILEEVAGQIKRRGIVVLVSDLLDEPAQILKGLRLFRFKGNDVIVFHIL
                     DQAELELPFEGNILFEDLEAANLNVIADPRAIRQTYRQVVDEFTSQMRKECHDSSIDY
                     QLISTSTPLDRALASYLSWRE"
BASE COUNT         1713 a         2200 c         2302 g         1799 t
ORIGIN      
        1 cgcccggcct agctttcgag gatctgccgc cgattcatgt cgtaatccta tcgcacgatc
       61 actacgacca cctggacttc gccacagtaa aacggcttgc cgatactcac aatccgcttt
      121 ttctagtccc tctcggtatg aaagcgtggt ttgcgaacga cggcatgaac cacgtcgagg
      181 aactcgactg gtggcaggag cgcgagtatc gaggtgtcaa gttcgtttgc ttgccggcgc
      241 agcatttctc acaacgaacc ttatgggacg gcaatgaacg actgtgggcg tcatgggcga
      301 tgttgagccc cgggcggcgg ctatacttta gcggcgacac cggctatttc gacggcttca
      361 aagaaatcgg ccacaagctt ggtccgttcg atctggcagc cgtcgctatc ggcgcctatg
      421 ttccgccgga gattatgaag agcgttcaca ccacgccgga ggaggcggta caagcgtccg
      481 tcgatctgaa cgcgcgcatt ctgctgggaa tccattgggg caccttcgat ttagccgaag
      541 agccgctgga ggagccgccg cagcgaatgc tggccgaagt tcgtcgccgc ggcatcgacg
      601 aggaccgggc atggatcttc aaaatcggcg aaacacggaa atggtagcgt agaatggttg
      661 ttcacagaga atcagggcag cttcggtgtt tgttagcctt acggattaca ccggggatgc
      721 gtgttggcga tttcgacttt ctggaacctt gtgaagtcac cgtccgccct ccgagagcac
      781 aacgatctcg accggctggg gcaactccgg tttggaaact ctgagaatgg ccgcgggtga
      841 acgcttcacc cgtggaaatc cgctaatgaa ctgatccgct gctcccgccc accagtaatg
      901 ctctggtatg gcgattcgcg gattgacctg tttggtgact tcaatggctt cgctaggcgt
      961 cacggtaaac atgccacccg ccaccggcac gagcagcacg tctattttcc ccaatagctt
     1021 aagctgtggc ggagtcagca ggtgaccgag atcgcctaga tgagcgatgc aaatgtcatc
     1081 cacgcgaaaa acgaagatgg cgtttttgcc gcgctgcaga ccctggctct tgtcgtgata
     1141 ggcgggaacc gagtaaaccg aaacgtcacg gatagtctca tgcaccggat tccaattttc
     1201 tccggtggga gtgaggccct ggaagatcat cgggttgcct ttggccatat caatattgtt
     1261 gtgcgcgcca tgttgatggc tggtggtgac aacatgttga tcgaggctcg gccagcgcaa
     1321 atcactgcgc gaatgcggat cggcaaggat gcgcgtgcct ttgctcgagg taatctgaaa
     1381 cgttgaatgg ccgaaccatt cgatttcaac cggccgggag ttggtggtcg ccactctgag
     1441 gatcggtctt tggaccactt ccatttcatg gcagaaagcc agcaccgttt gcggtaatgc
     1501 tgtcgtcgtc gcgatcaaaa gcaaaaccga ttgaagcaac tttgctggag acatctctcg
     1561 ctctgttctt agagtgatgc tgatacgtcg cgtaactatg agcctatagc agaccccatt
     1621 tgcgtcgcat ccaccattca acgcagagca acagcaggat cggcaagaaa gtccacaacg
     1681 tgctccaaag ttgcgtctgg cggcgctcaa cgatttgcga tggcgcgtgc tgctcaagct
     1741 ttgccgctat ttgctcgaca gccttttcat tccaatcggc aatcggaaaa taaaacccct
     1801 gactcgattc ggcgatcgcc tggagcagct cggtccgcgg cctgccatcg tcggtttcac
     1861 cgtaaggaaa tgctgtggag aagctcgatc ggtcttttcc caggggtttg ccgccgaggt
     1921 tggcctctgc ttcaacccgg taagaacctt cccttgtagg cgtgtattcg ccggtatatt
     1981 caccttcggc gctgtccgcc ttggctgcga tcaagctggg ctccccttca ggtccgaaca
     2041 cacgcacctg caccgcggcc tggcgtgtcg gggtaaaatc atccttcaaa acgcgcagtt
     2101 tgatagccag tttttccccg ggacgtgcca acggaatagg ttggatcttt acctgctcga
     2161 aggaaggttc ttgagccagc caacggactg attggcggat cagcttgaga tggttctgtg
     2221 gcgactctcg gttaccgacc gcgatgaagt tccagcgcca gatgtcatcg ctcatcaggg
     2281 ctaaagtccg tcctttaccg aagcggccaa ccgtcagcaa aggggatcct ttggcggcgc
     2341 cgtcgccgcc ggtcaccagc agagtctgac cgcgcgcggc gcgcacctga ttgatcgagg
     2401 tgagcggcgg tatcttggcc catgcgcttt cattcgcccg tggatcgggg aacaggcgcg
     2461 taatcggatg agcttttccc gaagcggtta acgccggcgg cacggcgatg tcgttgcggt
     2521 aggttccctt tccgtccatt tcgaccggaa gcacttcctt gagcgcgctt tcgccataac
     2581 cgccgctatc gaacgagcgc acgccgccga gcatcgccag gccgccgccg tcgcggacga
     2641 agtcctttac tttgtccagg tagaccggat tgaaataagc gcgatgcgag aagtcgtcaa
     2701 aaaaaaccac gtcgaagttc ttcagttcct ccagaaatat gtcgtcgatg ggaaacggga
     2761 tcaggctcag ctgcgagtca ggcacatcga cgctatcggt cggtgtgcgc aagaacacaa
     2821 aggagaccag ttcaatcagc gggtcctgct tcatcgccat gcgcaggaaa cgatagttcc
     2881 aggccggcga gccggatagc gtgagcacgc ggatcttgtc gcgctgaacg tcgactttaa
     2941 agtccttgtg attgttttgg ctgatttgct cgcccggctg cgccgggatg ttcaatgaaa
     3001 aatgatgcgg gccgatatcc ttgggagtga agctcaaagt gatcttacgc gtgaaatcgt
     3061 cgtcatcgat ggtaactgtc cggctggtga tcaggccgct gccgcgattg aaataaagcg
     3121 gcacggtttt tcctttcatt ccggatgcgc gcacagtgag gtcgaacttg aattcacgcc
     3181 cgcgaaacgc gaactcggga gttcgtaggt cggtcacgcg cacgtcggtg aagccctccg
     3241 tgtctccggc gccgacggta aacaccggca ccggcaacgc gctgcttccc tccaagtttt
     3301 gtctatcgcc attggcgatg ccgtcgctga aaattatgat ccccgactgc gggccggctt
     3361 ccttggcggc gatttgtagc agctccaata attgggttcc ctggtcttgc gctttgagct
     3421 gcggcaccga tgccggcgca atcggctcca ggctcgtgcc gaatcggtaa attcgtaaat
     3481 catactcgcg gctaagtttc tgaatgagcg gttcttcccc gtccgtcaat ttttgtttga
     3541 cgagatcgag gcggctcgac gccttgtccg gagcgctgcc ggggttggcg ggaaatgtca
     3601 tgctctgcga atcgtcaatc attaacgtga gcggccgccg cagttgcctg acctgctttt
     3661 ccaccaaagc cgggctcaaa agaaagaaaa tcaaaagacc ataaacgccg gcgcgcagag
     3721 aaaccaaaaa aatgctctgc ccgatgctca ggcgtcgtcg caaactgaga aactgctgga
     3781 ccagcaaggc gaggcaggcg gcgacaacca gcgcaaccag ccaccacgga acactgcttg
     3841 tgagatagag atccttcatc atcagcgcgt cagccgcttg cggataaacg ggacgtgaat
     3901 caaatcttct ttgtaattct ccgtcatggc gtagaggatc aaattgatgc cgaggtgaaa
     3961 tgcatcgcgg cgctgttcct ctccgcccgg ggtgcaggcg ttggtccatt tacccaattg
     4021 atcccgttcc caggcgcctc ccaggttgtt ttgacaatag acgaccggag tggaactgcc
     4081 gaccgtcact ccgtcgagag tggggctgac gattctgacg ccgccgatgc gtcggagaag
     4141 atagtagctc ctcagcgccg catgtccatt gggcaaccgt ttgaattcgt tgcctgggaa
     4201 gactcgctgc atctctcgcc ttattgactt gtcgaagccg taaccctgtt gacctaagcc
     4261 gtcatcgatc aagagaaaac cgccgaatga gagaaacaat cgcaaggtct cgatctcttc
     4321 agcggaaaaa ggttcgaatt cgtatctgcc ggtgatgtag agaaacggat aattaaaaag
     4381 atcgcgatcc gtcaggttca cctcatgcct ggccggggcc gcttcaacgc tggtgcgctg
     4441 catcaactct tccattaaag gagataccga taatggatgc gggttccagt cgccgccgcg
     4501 atatttgatc tgggcaaaga cgaattgcgt cggggttttc cctttgggat ggctggcgcc
     4561 ggcgaccgcc ggcaacagcc cggctgttac cagcgtttta gaaaggacgc aaagaaaatc
     4621 tcgcctgttc atcagcaatc gattctccgc agcaagaaaa ctaaatacgt tgcgcgaacc
     4681 agccttccaa taaaagcgtc gcgagcaaga gcccgagcag tggcagcgcg agatctcttc
     4741 ggatgccgcc ctgtcgcagg ctttcgatgg gcaccacgtc ggctcgaacc gggctgagct
     4801 tggcctgtag ttcgcgctcg ctgatctcct ccaggcgcga ttcgagaaac ggagggttga
     4861 ccgcgtacaa ccggggtgtg ccgctttcct gatcgccgtc ctttaacgcc agctggtaga
     4921 tgccagcaac atcgttatct tcaaaagaac cggaggcgcg atcttgcgct gcgttcatca
     4981 caacttcggc cggttgcttg ttgggcttgg tgatgccaat cgttttgccg acataaccgg
     5041 gcggcaagga gatctgtttg acgtcaccga cgctgattcc accatcgagc aggccgcgtt
     5101 taccgccggc cagatagttt gtcagcgatt ggatcatcgg cagataggct gttttgaccg
     5161 gcaggtcgct ccagtcccgg tccgccgtgg tggtaatgaa gataacccgg ccggagccca
     5221 ctttctgttc gatcaggagc ggatcgccgt ttgccagtga catcagcacg gatttgccgg
     5281 atgccgagat ccgagaatag ccccagatcc gcgccgactt gatcgattcc tgcaacaggt
     5341 tgtctgaaag actttgcagc gcgggatggg ttaattccat cttgccgatt ttttcaccgc
     5401 tagcttccaa accctgcttc ttgtcgcgaa tgggtgtctg gaggatctgc gccaatttgg
     5461 cgttgtagtg atcggcttgc aaccggtcgc cgccgaaaat caacaaaccg ccgcctgcgc
     5521 gcagataatt ctgcagcttg gcgataaagc tatcgggtaa gctggcgacg ttgcacaaga
     5581 cgattacctg ataggaatcc aatgcggctc cgctcagagc ttcgggaatg atcacggtgg
     5641 gcaggtactg cgacgagtcg ctctcgccgg cggggttgag cgcgcgggtt agaaagaacg
     5701 tttcgctttg gaccaatgat gtctggggat cgccatcgac gaccagcacc cttagctgat
     5761 cctgggcgtg cacgccaaaa tgcatcgaag cattgccggc gagcccatct tttttgatcg
     5821 tcaattgtcc ggtatgcgat cccgcttgag acaatcgagc ttgcaaggta atcgtggcat
     5881 ctcctcgggg cggaaccgac gtgagttttt gatctttgtt ttgcccgtcg atgctcagtt
     5941 ggactagcag ctccttgatt tcctgatcac tgaagtttgc cacgaccgct tccagatgca
     6001 gcggcaaatc gacaccgatg ccctgaccgg cggcgcggat ctctttcacg gctacgttca
     6061 gcggtttctg cttcctgccg acgcggatcg ccttgactgg gatcgccgga tcgacctgct
     6121 tcagtgaaga catcgaaaaa ttatcccagc cggtgaggcc gagatcggtg atcaggcgaa
     6181 tttccttttg accggcgggt tcgcccagga gctcataagc ctttgcgaga gccgttgaaa
     6241 aattggcggt gccgtcggca atctcgatct gatcgagctc cttcagcaga acatcttttt
     6301 cgactttgag acggatcggt tctttgccgg aaatgctggt gggaacgatt gcggcgcgat
     6361 cgctgtcgcc aagcgaggtg atcaacagcc gggccgcttc tttcgcttgc ttgaatccga
     6421 tgccgtcgcc gctccaggtc atactcagcg aattgtccag caagatcacc aatgaaaccg
     6481 gcccgccgcc ggcgaacaag ccggcgccgg tctgaaaaat cggattggcg agcagcaacg
     6541 ccaaaagcac cacggccatg gttcttagcg ccagcaatat ccaatggcgc aggcggtaac
     6601 tgcgcgaaat acgcttctgc gacatcagga taaaacgcac cgcgggaaag cggatgcggt
     6661 ttagcttgcg gcgattgagc aaatgaatca gcagcggtaa ggaggctgcg accagaccaa
     6721 aaaggaagat cggatagaga aagaaaaccg acatctcagc tcattccctc cagctcaagt
     6781 aactggccag cgctcggtct aagggcgtgg atgtagagat gagctgataa tcgatggagc
     6841 tatcgtggca ctccttgcgc atctggctgg tgaactcgtc gacgacttgc cggtaggttt
     6901 ggcgaatcgc gcgcggatcg gcgatcacat tgagattagc ggcttccagg tcctcgaaca
     6961 aaatgttgcc ttcgaacggc agttcgagct cggcttggtc gaggatatga aagacgatga
     7021 cgtcattgcc tttgaagcgg aacaagcgca gccctttgag gatctgcgcc ggctcgtcca
     7081 gcagatcgga gaccagaact acgatgccgc gccgtttgat ctggccggcg acctcctcga
     7141 gaattctgcc gacgttggtt tcgccgccgg gccgttggtt ttcaagcgcg ttaacgattt
     7201 gggtcaggta atcgcgcttg gcgcgcggcg gtatgatgtt ttcgatacgg tttgaaaagg
     7261 tcaccagacc ggcggcatcc tgctggcgca ggatgagata cgcgagcgaa gcggtcaggg
     7321 tacaaccgta atcgaatttg gtgatgccat cggatgagta gtccatcgat gcgctggtgt
     7381 cgagcagaag ataagcgcgc aggttggttt cgtcttcgta ttctttgatg aaatagcggt
     7441 cgaattttcc cagcgccttc caatcgatgc gccggatctc gtcgcccgga gagtattcgc
     7501 gatgttcttc gaattctacg ctgaagcctt tggagcgact gcggtgaatg cccgacataa
     7561 tgccttccac cacccagcgc gcccgcagtg agagattgga aatccccgcc aaaaccttcg
     7621 gatcgaagta ttttttctgc ccattggcga tcacgttgcc acttttacca ccgcggaaaa
     7681 agataatcta gccagcttct caagctgtgt ttcggcgccg cagattgcgc gtttggtatt
     7741 tgcgcgggct gaatagcggc gtgctggcgg tcacgtgact cgccgccggc cggacatcaa
     7801 tcggtctgag atgaaaatcg tgacgaggcg ggcgtacatg cttgtgcgtt agaggaccgc
     7861 aacttttcac atgatgctcg cgccgaatgc cacgtcttat catttaccag aacggtattg
     7921 attcaccgca gggctattgt ttaggtgaca ataggagatg cggtccttag atctgagggt
     7981 cgtctacaac taaaccgttg tgttactcgt tgcc
//
LOCUS       DASUVS010000371        13135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1687692, whole genome shotgun sequence.
ACCESSION   DASUVS010000371 DASUVS010000000
VERSION     DASUVS010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13135
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687692"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(599..1231)
                     /locus_tag="VF182_19915"
     CDS             complement(599..1231)
                     /locus_tag="VF182_19915"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6439391.1"
                     /translation="MQNYVYLIGSTETRKAAVVDAAWDIDEILRIAAQDEMEITHALV
                     THTHPDHVGGGFAGMEIAGATELLDKCKAKVVVHKAEAEFIKGLSPSDLIKTDNGDKV
                     DVGGLEVQLMHTPGHTPGSQCFLVENRVVSGDTLFIDACGRVDFPGGNPEQMYYSLTQ
                     KLMTLPDDMILFPGHNYAPLKHATIGEQKKTNPYLKFSSLKQFLAAMGYR"
     gene            complement(1391..1831)
                     /locus_tag="VF182_19920"
     CDS             complement(1391..1831)
                     /locus_tag="VF182_19920"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6439392.1"
                     /translation="MAIKEVTPQQAQEILTNDSSAIYIDVRTETEFANGHPQGAVNIP
                     VAFPDPTRGMMLNSDFIKVVEANFPREKKIILGCQAGPRSTSAANMLQQAGFQDVSNL
                     LGGFGGMRDPTGRISAPGWASSGLPVSNDNGEGVSYQSLSAKAR"
     gene            complement(1931..2701)
                     /locus_tag="VF182_19925"
     CDS             complement(1931..2701)
                     /locus_tag="VF182_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEX6439393.1"
                     /translation="MGLLDGKKAAVFGVANDRSIAWAISEALHAEGADLAFTYAGEIL
                     EKRVRPLAEGIGSKIILPCDVTKDEEIEKVFTTLKQEWGALDIMIHAIAYAKKDDLAN
                     PYVQTSRQGFHLALDVSAYSMVALARHAAPLMEGRGGSIVTLTYMGSERVIPNYNVMG
                     VAKAALEASVRYLAHDLGPKGIRVNAISAGPIRTLAASGIADFKEMLHHVSERAPLKR
                     NIDAEEVGKTALFLCSAWGSAITGEVLHVDAGYSIMGK"
     gene            complement(2843..3451)
                     /locus_tag="VF182_19930"
     CDS             complement(2843..3451)
                     /locus_tag="VF182_19930"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX6439394.1"
                     /translation="MAIRITRVYTRTGDKGETSLVGGKRVPKDSPRIDAYGTIDELNS
                     IIGLARVFNEENLDAGEAHQFLDEVLCKIQDELFDLGSELATPPEFFQEGMFRISQNE
                     VKNIEKLIDKCQENLQPLESFILPGGGRIGAYLHQCRTVCRRAEREILRLSREEPISE
                     WPLKYVNRLSDLFFVLSRWISKETGEREYLWQRGLQSNKKGG"
     gene            complement(3487..4878)
                     /locus_tag="VF182_19935"
     CDS             complement(3487..4878)
                     /locus_tag="VF182_19935"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX6439395.1"
                     /translation="MAQQVLKRQIGLFSAVMLIAGDMIGTGIFISTGVIAETLPSPGG
                     VLLVWLLGGLLALTGALTFAELSASLPFAGGDYIYIREAYGKLMGFLSGWSSFLVTFS
                     GAIAFLAVSLNGFMAFFFPVLRSEAVVFSLPIPWLPISATAGTLFAIAVVLMLSGLHC
                     LGVRQGTMVQNILTILKIAALLGIIIFGILFGKGNTAHFVPLFDWQKVSFSVFAAAFI
                     PVIFAYSGWNAVTYIAGEVKNPERNLPRALLFGVLIVIALYLAINAVYIYAVPVTEMK
                     GALRVSEVATTALFGYQTSALITAIITISILGALNVVTMIGPRIYYAMAQDGVLFKSL
                     SRIHPRFGTPTSAIILQAIWTCLLILTNTWGTLFTYVSVVITLFSAFTVGSVIVLRYK
                     RPELKRPYKVWGYPLVPLLFVLINLWIVWGSVKEKPFESLVGVAIVAIGIPIYFVWKT
                     LGPAQKPSFASRS"
     gene            5081..5764
                     /locus_tag="VF182_19940"
     CDS             5081..5764
                     /locus_tag="VF182_19940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6439396.1"
                     /translation="MKTLITLLALLPMPFSAPAQDAVKRDHQQMHQLHRDSRAYIGML
                     EDPKRDAYQKPHEVLMALAIKPGEAIADIGAGSGYFTFRLAHHVGDKGKIYAVDVNPE
                     MIVHLNRRIRELKAMNVTAILADPDDPLLADNSIDRFFFSDSWHHIENQTKYLSKIKK
                     MLKPSGEVVMIDFHKKELPVGPPMQMKIDREDLIRQMGSNGFRLTKEHTFLPYQYFLV
                     FMPNQHRER"
     gene            5966..7261
                     /locus_tag="VF182_19945"
     CDS             5966..7261
                     /locus_tag="VF182_19945"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX6439397.1"
                     /translation="MQTRWLSSIMVIFAIFVLGSNFSSAWGDDDDGRPFPNSTFTTLI
                     TTPRALEGLTGDNHRNLYSGGSGVPPCPIWQINLNKPALIPVGVVVPATGNCGFSGIA
                     FNEIGDLFVADGAAGNIYTFKPNANTPPTATVFASGAPGANGIAFDKKGNLWASDGTT
                     GRGRVWKISGPGANCSSTAAATNCEEVFRIQPMSNEVNLDASGVGGVGRDIRDLAPGT
                     ITVTPTTRNAANQLGSQPIVANGLAFNKKGDLFNIDTARGALWKMEFDRDGNLKSPIG
                     CDSTFTANTLCLSNVVVAHPILEGGDGIALDRAGNIWVAANERNAVAVVTKDGKVIEV
                     FRNPVNLPQPVSLGVGLRNAGDQSVGNNRILEFPTSPFITGRVLCISQSDGDRRDNSP
                     RSAGEVNAGATFDGKNLLGKISCMDQELKIPGLPLPIDD"
     gene            complement(7394..8893)
                     /gene="lnt"
                     /locus_tag="VF182_19950"
     CDS             complement(7394..8893)
                     /gene="lnt"
                     /locus_tag="VF182_19950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX6439398.1"
                     /translation="MSSKLYSLIVAAILLAAPYLAPSLFPLAWIAFVPLFRAITQAET
                     LRRAVLYGWMTGTIAHLIGFHWLVYTINVFGGFPYSISTLVFLVYAILQGAQMAIFAS
                     LVRRFGFGPLQILPAVFWVALEFWFPLLFPWYLANSQVSFSLFIQTADLVGPYGASFL
                     LMWFNAAAFRIVYPGDEARRSHLPALAYCAVIVVMSLVYGYQRLGAVGQEIVAAPKLP
                     VAAVQGNIDIDLKWNPALVKQNLEKHKKLTSQLDAVALVIWPESAVEVWLPDQLQYLP
                     ADLLPPLSDQSYFIFGAKSFRGKPGTANFMAFNTAFFTDAAGRVSSRYHKQVLLAFGE
                     YLPFAKVLSLLPAMPFADGFTAGNGPVAFEMPGGIRIGSLICYEDLMPELSRNFVKES
                     SANLLVNLTNDAWYGASVGPWQHARLAQSRAIETRRSLLRVTNTGVTSLVNAKGELVQ
                     SLPMFVPDVLKTSVDILDGETYYVRYGDWFAWGMTIITVAIVPFHLKRA"
     gene            8995..10671
                     /locus_tag="VF182_19955"
     CDS             8995..10671
                     /locus_tag="VF182_19955"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HEX6439399.1"
                     /translation="MESTQRKLVSLAAAILMTVVFGGSAVAQSVLSDDWEKFFNEKVS
                     FGGFVENTTGFSISHGSRFFNTSNRFIMNRFTIQPEFNFEFTESFKLFLSWRFVAEPR
                     YSAETKSREAAVQPAGTGGALPVDFYSEYKGVPWEAVADILPTDRLHLRIGRQFISWG
                     ETDGQRLLDVINPQNLTFSPPAAVNLFSLEESRVPQWGVRMLYTVRPVSNTILELFGN
                     PGFENKKDRVDEFAPTNDISDGKADGDLRYGRWSGHPESRMPVARLLANPVGPAPVVV
                     PAFKRDYPDAGDAWKIGGRITHSFGELNAGIGYIRGYNPQANDMVFKMKGAPISCGPP
                     ACPPGGTLVRADLVNNQTNIFAAHFNYPVGEPLGIPIKSAMRGELAFYPDKPYNIGEF
                     PGRNCNTGAPTGLRAGPSCEHSDNVVEKHTIRYALGFDRSTFIPFLQDDPWRPFRMSL
                     QIFQSIILDHEDGIRAFGHATQIERFSTLFTFRIGTGYLGDTILPDVFVGYDPAGYYS
                     VNPAITYQPPWNEKIQLTLTSAIYGGHNKFGGLGLFSEKDSVFLKMRYQF"
     gene            10695..11948
                     /locus_tag="VF182_19960"
     CDS             10695..11948
                     /locus_tag="VF182_19960"
                     /inference="COORDINATES: protein motif:HMM:NF018716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HEX6439400.1"
                     /translation="MKLIARSRGLVARNGAAACIFLYIAMFLSFPGITPAAETDLKPG
                     DTIGPQNWQSIQGMVGENLLNRIKGGYTIQIKPTKTYRPLKEYVEATEKYSGKVSLGP
                     NGELLNYVAGQPFPKFDPNDPQVGQKLAWNFFYRWLGDDYKTGGAVKGGKIIRAAIEK
                     DGSERRADLVSYFLFPSTRYSLNPKPAIPGYEHIDFMQLRVDSYPRDASGTTTLEIRY
                     KDPGRADDLYIYLPSIRRIRRATTTQRCQTLAPSEFNLDDINSFNGKITDFNYKYLGD
                     KKILANISQEHLPFARKPGDYLPLDEKWEVVDTYVLEVTPKDSSYCYPKKVLHLNKVT
                     FDTHWTLMWDSKGAYWKEQFGFFTPVKLADGREVWSVGDVVIHNVQNGRSTIVTATRA
                     YNQGYPASMFSLGTLQSIMRGGSIE"
     gene            12008..13039
                     /locus_tag="VF182_19965"
     CDS             12008..13039
                     /locus_tag="VF182_19965"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX6439401.1"
                     /translation="MHLLFFFLIWVLLGLAPVVLPAHAATLSKEPALQENFYGVHISD
                     NLSWIVGYYGTILHSIDRGKTWEIQPSPTRQALFTVRFVSPLSGWISGSYGTLLRTDD
                     GGKSWHAQSLNITEHLFASHWLDESRGWLAGNRGMTIHTSDGGHSWSKTVVPGDFTFN
                     AVSFASASRGWLAGEFGVIFQSSDGGKSWAKQKSPVEVSFDSGASQNLFALLFTAPSN
                     GYAFGLDGVVLNTTDGSRWEIVRQRENTDSTTATHHLFAAAEFNGRIWATGERGTLLQ
                     SDVDHAQWRQAELQIPRASLNAIAFGKDGFGLVVGNRGLVLRTEDGGKSWQRLKIITR
                     SLENHQSAR"
BASE COUNT         3166 a         3528 c         3446 g         2995 t
ORIGIN      
        1 catcgtcaaa gaacgcgcca aggcgccgtc ccctaaagaa ggcgtgctgt ggacgaacaa
       61 gtatgcaggg aagtaacagg gttttggtct tgggtattgg atattgggtc tgtgtgatta
      121 gcaattcggt ggtgactgac ggcttcgcaa cacaattgag ttgttttccg ggcaccccga
      181 atcacagtcc gggggggcgg gggaacgcga accggatcca gaaatcttcg tagcggcaac
      241 aactttctta tttgagattc ttagacagaa cgctaacaat cggaagcgtg taaactgaga
      301 gaactttgat cgatctgttg tgagtggctc ggtcaaaaaa tccttgcgag ccatcgctcg
      361 ccgcaggttg caagtcgctg taatcagccc atacaacgca gactcttaag actgtgagtg
      421 caaaattgtt tagttactgc tctttagcta ggcatgaacg acctgttttt tgagatcgca
      481 ccaaggccga acgattaaga agccgcgacg gagagtggca caggatttct aatcctgtgc
      541 cgtacgttta ctcaacgcgt ctacaagcgg cgatctcatt cggctaaccg gcagccgatc
      601 aacgatagcc catcgccgcc aggaactgtt tcaacgagga aaatttcagg tagggattgg
      661 ttttcttctg ctcgcctatg gtggcgtgct tgagaggcgc gtaattgtgt ccaggaaaca
      721 gaatcatatc gtcgggcagc gtcatcagct tctgagtcaa gctgtaatac atctgctcgg
      781 gattgcctcc aggaaaatcc acgcggccgc acgcatcgat gaataatgta tctcctgaaa
      841 cgactcggtt ttcgaccaaa aagcactgcg agcccggtgt gtgtccgggg gtgtgcatga
      901 gctgaacttc caggccgcca acatccactt tgtcgccatt gtcggtcttg atcaggtccg
      961 acggcgacag gcccttgata aactccgcct ccgccttgtg cacgacgact ttggctttac
     1021 acttatcgag caactcggtc gcgcctgcta tctccatgcc ggcaaatcca ccgccgacgt
     1081 gatcgggatg agtgtgagtc acgagagcat gcgtgatttc catctcgtcc tgcgccgcaa
     1141 ttcgaagaat ttcatcgatg tcccaggccg catcaaccac cgctgccttg cgggtttccg
     1201 tcgagccaat gaggtacacg tagttctgca tcgggccgat ttcgatctgt ctgaaataaa
     1261 gtgaatggtt cattgccgcc cggaaagaat tacttcgtgc ttcagcttct gtaaaacttt
     1321 ctatttctcg tccaccgcca gcaagcgtga tgtaatttcg aggagcgaga atctttcttg
     1381 aaccggcttg tcagcgagcc tttgccgaca gtgactggta gctgacgcct tctccgttgt
     1441 cgttgctgac cggcagcccg gagcttgccc agcccggagc gctgattctt ccggtcggat
     1501 cgcgcatgcc gccgaagcca cccaacaagt tagaaacatc ttgaaagccg gcctgttgga
     1561 gcatattagc cgccgatgtg gagcgcggtc ctgcttggca gccgaggatg atttttttct
     1621 cgcgtggaaa attcgcctcc acgactttga tgaagtcgct gttcagcatc atgccgcgcg
     1681 tcgggtcggg aaaagccacg ggaatattca ccgctccctg tgggtgaccg ttggcaaatt
     1741 cggtttcggt acgcacgtcg atgtaaatag cgctcgagtc attggtgaga atttcctgag
     1801 cctgctgcgg cgtgacttct ttgatcgcca tgaaacacct cgccagagtg ctggactgct
     1861 aggatgctgg agaacaaggt gagtcggagc aacagtccat tgcccataat tcattcgccc
     1921 agcttacctg ctacttcccc atgatactgt agccggcatc gacatgcagc acttcgccgg
     1981 tgattgcgct gccccaggcg ctgcaaagaa acagcgcggt tttgcccact tcttcggcat
     2041 cgatgttcct ctttaaaggc gctcgctcgc tgacgtgatg aagcatttct ttaaaatcgg
     2101 cgatgcctga agcagcaagg gttctaatcg gtccggcgga aatcgcattg acccgaatcc
     2161 ctttgggccc gagatcatgc gccagatatc ttacgctcgc ttccaatgcc gctttcgcga
     2221 ctcccatcac gttgtaattc ggaatcaccc tctcagaacc catgtaggtc aaggtcacga
     2281 tcgagccgcc ccgtccctcc attagcggcg ccgcgtggcg cgccagcgcg accatcgaat
     2341 acgcgctcac gtccagcgcc aggtgaaatc cttggcggct ggtttgaaca tacgggttgg
     2401 ctaaatcgtc ttttttggcg taggcgatgg cgtggatcat gatatccagc gcgccccatt
     2461 cttgtttgag agtggtaaaa accttctcga tttcttcgtc tttagtcaca tcacacggca
     2521 agatgatctt ggagccaatg ccctcggcta acggtcgcac ccgcttctcc aagatctcgc
     2581 ccgcataagt aaaggctagg tccgcgcctt cggcatgcag cgcttccgaa atggcccagg
     2641 caatactccg gtcatttgcc actccgaaga ctgccgcttt cttcccatcc aaaagcccca
     2701 tcaaagactc ccccgatcca ttttcgattt agattttgga tttgcgatga tcggatgctg
     2761 atatcagatt ttcgcagcaa aacttttcat acttccgttt tccgttattc cccatcgaaa
     2821 atcggcaatc caaaatctaa aattatccgc cttttttgtt gctttgaaga cccctctgcc
     2881 acaggtattc tctttctccg gtctccttgc tgatccaacg cgacaggaca aagaacaaat
     2941 cgctcaaacg attgacgtat ttcagcggcc attcgctgat tggctcctcg cgtgagagcc
     3001 gcagaatttc tcgttccgct cgccggcata cggtgcggca ttgatgcaga taagcgccga
     3061 tacggccgcc gccagggaga atgaaagact caagcggctg gagattctcc tggcacttgt
     3121 cgattagctt ttcaatgttt tttacttcat tttgggaaat gcgaaacatg ccttcctgaa
     3181 aaaattcagg cggtgtggct aattcgctgc cgagatcgaa tagctcatct tgaattttgc
     3241 agagcacctc gtccaggaat tgatgcgcct cgccggcatc aaggttttcc tcattgaaca
     3301 cgcgggccaa tccgatgatg gagttcaatt cgtcgatcgt gccgtaagca tcgatacgcg
     3361 gcgaatcctt aggcacgcgt ttgccaccta ccaggctagt ttctccttta tcgccagtcc
     3421 tggtgtaaac tctggtgata cggattgcca ttggcgaatg gttctcgtta ttgcggaact
     3481 agcgtcttac gatcttgaag cgaaactcgg cttttgcgcc ggacccaggg ttttccaaac
     3541 gaaatagatc gggataccga tggcgacgat ggctaccccc acgagtgatt cgaatggttt
     3601 ttctttcacc gagccccaga caatccatag atttatcagg acaaagagaa gcggcacgag
     3661 cgggtaaccc cagaccttgt aaggtcgctt gagctccggt cttttgtagc gaagcacgat
     3721 cacggaacca acggtgaatg ccgagaatag cgtgatgacc acgctcacat aggtaaacaa
     3781 ggttccccag gtgttggtta aaattaataa acaagtccaa attgcctgga ggatgatcgc
     3841 gctggttgga gtgccaaagc gcgggtgaat gcggctcagg ctcttgaaca gaacgccatc
     3901 ctgcgccatc gcataataaa ttcgcgggcc gatcatggtc acgacgttga gcgcgccgag
     3961 gatggatatt gtaatgatcg ccgtgatcaa agccgacgtc tgataaccga ataatgcggt
     4021 ggtcgccact tccgatacgc gcagggcgcc tttcatctcg gtaaccggca cggcatagat
     4081 atagacggca ttgatagcca agtagagtgc gatgacaatg agtacgccga atagcaaagc
     4141 ccgaggcagg tttcgctccg gatttttcac ctcgcccgca atataagtaa cggcgttcca
     4201 gcccgaatag gcaaagatta ccggtataaa ggcggcagcg aatacgctga agctaacttt
     4261 ttgccaatca aagagcggca caaaatgcgc cgtattgcct ttgccgaaga gaatgccaaa
     4321 aataatgatt cccaagagcg cggcgatttt taaaatcgtc agaatatttt gcaccatcgt
     4381 gccctgacgc acacccaggc aatgcagtcc cgataacatc aggacgacgg caatcgcaaa
     4441 tagcgtccct gctgtggcgc tgatcggtaa ccacggtatc ggcaatgaga atacgacggc
     4501 ttcggaacgc agcacgggaa agaagaaggc catgaacccg tttaaactta cggccaaaaa
     4561 tgcgatggca cctgagaacg ttaccagaaa cgaggaccaa ccggagagaa accccatcaa
     4621 tttaccatag gcttcgcgga tatagatgta gtcgccgccg gcaaacggta gactcgccga
     4681 aagctcagcg aaagtaagcg ccccggtgag agctagtagt ccgccaagca accacaccaa
     4741 cagcacgccg cccggcgacg gcaatgtctc ggcgatgacg ccggttgaga tgaagatacc
     4801 ggtgccgatc atatcgccgg cgatcaacat gaccgcgctg aacaaaccta tttgccgctt
     4861 caacacttgt tgcgccatag cgattaccca tagcagggaa gcggctaagc tcgcaacaga
     4921 tttaggcctt ggagctcatg gaaaatgtgt tagcttcacc gcattgtgag cgaagatctt
     4981 cggcgcgcaa actgatgtcc tggttccttc gtttaacgat caggcaggtt cttggatcgc
     5041 tgcgtacagc gctgagcgcc agcgcgccaa tgaggtgaaa atgaaaacgt tgataactct
     5101 tttggctcta ctgccgatgc cgttctcggc accggctcag gatgcggtca agcgcgatca
     5161 tcagcagatg caccaacttc accgggactc aagagcctac atcggaatgc tcgaagatcc
     5221 gaaacgggat gcctatcaaa agccgcatga ggttttgatg gcgctggcca tcaagccggg
     5281 tgaagcgatc gccgatattg gcgcaggctc cggttatttc acatttcgcc tggcgcacca
     5341 cgtgggcgac aaagggaaga tctatgccgt cgacgtcaac ccggaaatga ttgtgcatct
     5401 caaccggcgc atccgggagc tcaaagccat gaacgtcaca gctattctcg ccgatccgga
     5461 tgacccgctc ctggccgaca attccatcga ccgcttcttt ttttcagatt catggcacca
     5521 catagagaat caaacgaaat atctctcgaa aattaaaaaa atgcttaaac ctagcgggga
     5581 ggtcgtcatg atcgactttc acaagaagga acttcccgtc ggcccgccga tgcagatgaa
     5641 gattgatcgg gaggatctga tccggcagat gggaagtaac ggctttcggc ttacgaagga
     5701 gcacacgttc ctgccgtacc agtattttct ggttttcatg ccgaaccagc atagagaacg
     5761 atgacggtcg tcagtaacga gcggttctgc cccggtcgtg tcgccttgtc gcgaccggct
     5821 gatcaacgcc atcaacaaac gttcatctca ctcatggaac tctatggaaa acggcttgac
     5881 acgtcatata cgttacggga taagctcgcg ctcgttaacc acctgaagca aatgcttgcg
     5941 tgctgaaaca ggaaggagga gtcttatgca aaccagatgg cttagttcaa tcatggttat
     6001 ctttgccatc ttcgtcttgg ggtcgaattt ttcctcggcc tggggagacg atgatgatgg
     6061 tcggccgttc ccaaactcca cttttactac cctgatcacc actccacgcg cactcgaggg
     6121 gttgacgggt gataatcacc gtaatctcta ctccggcggc tcgggcgtgc ctccgtgtcc
     6181 catttggcag ataaatctga acaaacctgc attgatccct gtaggggtcg ttgtcccggc
     6241 aaccggaaac tgcggcttct cgggtatcgc ttttaacgag atcggcgatt tgttcgttgc
     6301 cgacggagcc gcgggaaata tctacacctt caaaccgaac gccaacactc cacccacagc
     6361 gacggttttt gcctccggtg ctcccggagc gaatggaatt gccttcgaca agaaaggcaa
     6421 cctctgggcg agcgacggga caacggggcg gggacgggtt tggaagatat ccggtcctgg
     6481 agccaactgc agttccacgg cggcggcaac taactgcgaa gaagtctttc ggatccagcc
     6541 gatgtcgaac gaggtgaatc tagacgccag cggagtgggt ggcgtgggac gtgatatccg
     6601 cgatttagct ccgggaacga ttaccgtcac gccaacaacc cgcaatgccg ccaatcagtt
     6661 gggctctcaa ccaatcgttg caaacggctt agcgttcaac aagaagggcg accttttcaa
     6721 tatcgataca gctcggggag cgctttggaa gatggaattc gatcgcgatg ggaacctcaa
     6781 gagtccgatc ggatgcgata gcacattcac agctaacacg ctgtgcctca gtaatgtcgt
     6841 cgtcgctcat ccgattctcg agggcggcga tggcatcgcc ctcgatcgtg cggggaatat
     6901 ctgggtagct gccaatgaac ggaatgcagt cgctgttgtt acgaaagatg gaaaggtcat
     6961 tgaggttttc cgtaatcccg ttaacctacc gcagccagtg tcattaggag ttggtttgag
     7021 gaatgcgggc gaccaatccg taggcaacaa ccgcatcctc gaatttccaa ccagcccctt
     7081 tatcacagga agggttttgt gcatttctca atccgatggt gaccggcgag acaattcccc
     7141 caggagcgcc ggtgaagtga acgccggcgc aacttttgac gggaaaaatc tcttagggaa
     7201 aatttcctgc atggaccagg agctgaaaat cccgggtctg ccactgccga tcgatgacta
     7261 gtttttaaac gatggcaaac ctagaaaaac aggcgctata aaaacatctt tgtggcatct
     7321 ggtttcgtcc tctggcgttg tttttttgtt tgccggtctc tcggccgaga tctagctaac
     7381 gccgtttaac cggtcaggct ctcttcagat ggaaaggaac aatcgcgacc gtaatgatgg
     7441 tcatgcccca ggcgaaccag tcgccataac ggacgtagta ggtctcacca tccaaaatgt
     7501 cgacgctggt ttttagcacg tccggcacaa acatcggcaa agattgcacc agttctcctt
     7561 tggcattgac caatgacgtc accccggtat tggtgacacg gagcaaactc cggcgggttt
     7621 caatggcgcg ggattgcgcc aggcgggcgt gttgccacgg tccaaccgat gcgccgtacc
     7681 aggcgtcgtt ggtgagattg accaggaggt tggcgctact ttctttgaca aaattgcggg
     7741 acaattcggg catcaaatcc tcgtagcaga tcagcgagcc gatgcgaatg ccccccggca
     7801 tctcaaacgc aacgggaccg ttccctgcgg taaaaccgtc ggcgaacggc attgccggca
     7861 gcaacgacag caccttggca aatggcagat actcgccaaa cgcgagcaga acctgtttgt
     7921 gatatctcga cgagacccga ccagccgcat ccgtgaagaa tgccgtatta aacgccataa
     7981 aattcgcagt accaggcttg ccgcggaagc tttttgcacc gaagataaag taggattggt
     8041 cggacagcgg cggcaacaaa tcggcgggca gatactgcaa ctgatcaggt aaccagacct
     8101 caactgccga ttccggccag atcaccagag ccacggcgtc caactggctg gttaatttct
     8161 tgtgcttctc aaggttttgc tttaccaagg ctggattcca cttgaggtcg atgtcgatat
     8221 tcccctgcac cgccgccacc ggcaacttcg gcgccgcgac gatttcttgc cctacagcac
     8281 ctaaacgctg gtaaccataa accagcgaca tcaccacaat tacggcgcaa taagcgagcg
     8341 ccggtaagtg cgaccgacgc gcctcatcgc cagggtaaac gattcgaaac gccgcggcat
     8401 taaaccacat cagtaaaaag ctcgccccat aaggaccgac cagatcggcc gtttgaatga
     8461 acagcgaaaa tgaaacctgg ctgttggcga gataccatgg aaacagcagc gggaaccaga
     8521 attcgagcgc aacccagaag accgccggca gaatctgtaa cggcccaaat ccaaaccggc
     8581 gcacaagaga ggcgaagatc gccatctgtg ctccctgcaa aatcgcatag acgaggaata
     8641 cgagggtact gatcgaatag ggaaagccgc cgaacacatt gatcgtgtag acaagccaat
     8701 gaaacccgat caaatgagcg atagtgcccg tcatccagcc atacaaaacc gcccggcgca
     8761 gagtttccgc ttgagtgatg gcccggaaga gcggcacgaa ggcgatccaa gcaagtggaa
     8821 ataagctagg ggcaagataa ggagcggcta acagaatggc tgctactatg agcgagtata
     8881 acttgcttga catattaatt ttcaaactat aaatgaataa tcccatctat ctgtaaaaag
     8941 ttatcacgaa ctatcggtga aaacataatc acccgagcta cgcggaggcg catcatggaa
     9001 tcgacgcaac gaaaacttgt gtcgctggct gcggcaattc ttatgaccgt tgttttcggc
     9061 ggatccgctg tcgctcaatc ggtacttagc gacgactggg aaaaattctt caatgaaaaa
     9121 gtctctttcg gcggattcgt tgagaatacg accgggttct ccatcagcca tgggagccgt
     9181 ttttttaaca catcgaaccg gttcatcatg aaccggttta caattcagcc ggagtttaat
     9241 tttgaattca cggaaagttt caaactgttc ctttcctgga gattcgttgc cgaaccccgc
     9301 tacagcgcgg aaaccaaaag ccgtgaagca gccgtccaac cggccggaac cgggggagcc
     9361 ttacctgtgg acttctattc ggagtacaaa ggtgttccct gggaggcagt cgcagacatt
     9421 ctacccacgg atcggttgca tttaagaatc ggtcggcaat tcatctcatg gggggaaacg
     9481 gacggacaga ggcttttaga cgtcatcaat ccccaaaatc taacgttttc cccacccgct
     9541 gctgtcaatc tctttagttt ggaagaatcc cgagttcccc agtggggagt cagaatgttg
     9601 tacaccgtac gcccggtcag caatacgatt ctagaacttt ttggcaatcc cggtttcgaa
     9661 aataagaaag atcgagtcga tgagtttgcg ccgaccaacg atatcagcga cggcaaggcg
     9721 gacggcgacc taagatacgg acgatggtcg ggccacccgg aatcccgcat gccggttgcc
     9781 cgtcttttgg ccaatccggt tggacccgct cccgtcgtag tgcctgcctt caagagggat
     9841 tatcccgatg ccggcgatgc ctggaaaatc ggtggacgga ttacgcacag ctttggtgag
     9901 ctaaatgccg gtatcggtta catacggggt tacaatcctc aggccaatga tatggttttc
     9961 aaaatgaaag gtgccccgat ttcttgcggg ccaccggcct gtcctccagg cggaaccctg
    10021 gtgcgggccg acctggttaa caatcagacc aacattttcg ctgcgcattt caattatcca
    10081 gtcggggaac cgctgggtat tccgatcaaa tctgcgatga ggggcgaact cgctttttat
    10141 ccggacaaac cttacaacat cggcgagttc ccaggccgaa attgtaacac cggtgcgcca
    10201 acgggtctca gagccggccc tagctgtgag cattccgaca atgtcgtcga gaagcatacc
    10261 atccgctatg ctctgggctt tgaccgttca acgtttatcc cgtttcttca ggacgatccc
    10321 tggcgaccgt ttcgaatgag tttgcagatc ttccaaagca taattttaga ccatgaggat
    10381 ggtattcggg ctttcggcca cgcaacccaa atcgagcggt tttcgacgtt atttaccttt
    10441 agaatcggca cgggatatct aggcgatacg attctgccgg atgtttttgt cggctatgat
    10501 ccggcggggt actattcggt caacccggca ataacctacc agccgccctg gaacgagaag
    10561 attcaactga ccctgacgag cgccatctac ggagggcata ataagtttgg tggtttaggg
    10621 cttttctccg aaaaagattc ggtctttctg aagatgcgct atcaatttta ggtttacgat
    10681 tgggagattt tgagatgaag ctaatagccc ggagtcgcgg cttggttgct cgcaacggcg
    10741 ctgccgcgtg tattttcttg tacatcgcta tgtttttgtc ctttcctggg atcactcccg
    10801 ccgcggaaac cgatttaaag ccgggcgaca ccattggtcc acaaaattgg cagagtatcc
    10861 agggcatggt cggcgaaaat ctcctcaatc ggatcaaagg cggttatacg atccagatca
    10921 agccgaccaa gacctaccgg ccgctgaagg aatacgttga agcaacggaa aaatactccg
    10981 gcaaggtcag cctcggccct aatggagagt tgctcaacta tgtggccggc cagccgtttc
    11041 ccaagttcga tccgaacgat ccccaggtcg ggcaaaagct agcttggaac tttttctatc
    11101 gctggttggg cgacgattac aaaaccggcg gcgcggtaaa aggcggcaaa atcatccgcg
    11161 ccgccatcga gaaagacggc tctgaacgcc gcgcggactt ggtatcgtat tttctttttc
    11221 cgagcactcg ctacagcttg aatcctaagc cggcaattcc aggctacgaa catatagatt
    11281 tcatgcagct tcgagtcgat agctatccgc gcgacgcttc cggcacgacg acactggaga
    11341 ttcgttataa agatcccggc cgggccgacg atctctacat ctatcttccg agcatccgca
    11401 gaatccggcg cgccaccacg acccagagat gtcaaacgct ggctccgagc gaattcaacc
    11461 tcgacgacat caatagcttc aacggcaaaa ttactgattt caactataag tacctgggcg
    11521 acaagaagat cttggcgaac atcagtcagg agcatctccc gttcgcgcgc aagccgggcg
    11581 attacctgcc gttggatgag aagtgggagg tcgtggatac ctacgtgctg gaggtcactc
    11641 ccaaggactc gagctattgt tacccgaaaa aagttcttca tctaaataag gtaactttcg
    11701 acacgcactg gacattgatg tgggatagca aaggagctta ttggaaggaa caatttggtt
    11761 ttttcactcc ggtaaaactt gccgatggcc gcgaagtttg gtcggtgggc gatgtggtta
    11821 ttcacaacgt tcagaatggc cgaagcacga ttgttaccgc cacgcgcgct tataatcagg
    11881 gttatccggc cagcatgttt agcctcggaa ccttgcagag catcatgcgc ggaggatcga
    11941 ttgaataatg tcgagctgag ttgatggatc ctaatctttt ttcagcttcc gaatttaaat
    12001 ccgctccatg catttgctct ttttttttct catatgggtc ctcctgggat tagctccggt
    12061 agttcttcct gctcatgcgg caactctctc gaaagagccc gcgctccaag agaatttcta
    12121 cggagttcac atttcggaca atctctcttg gattgttggc tactacggga ccatcctcca
    12181 ctccatcgac cgtggtaaga cctgggagat tcaaccgagc cctacgcgcc aggctttatt
    12241 cactgttcgt ttcgtcagcc cgttaagcgg ctggatcagc ggcagttacg gcacgctttt
    12301 gcggaccgac gatggcggca agagctggca tgctcagtcg cttaatatca cagagcatct
    12361 gtttgcttca cattggctgg acgagagccg cggctggctg gccggcaacc ggggcatgac
    12421 gatccacacg agcgatggcg gtcactcctg gagcaaaact gtagtgcccg gcgacttcac
    12481 ttttaacgcg gtcagcttcg ccagcgcctc gcgcggatgg ctcgccggtg aatttggggt
    12541 catttttcag agctcggatg gtggtaagag ctgggccaag caaaaaagtc cagtagaagt
    12601 ttcgtttgac agcggcgcaa gccaaaattt gttcgcgctg ctattcacgg cacctagtaa
    12661 cggctatgcc ttcgggctcg acggagtcgt gctcaatacc actgacggat cgcgctggga
    12721 gatcgttcgc cagcgggaaa acaccgatag cacaaccgca acccatcatc tcttcgccgc
    12781 agccgagttt aacggacgca tttgggcgac cggtgaaaga ggcacgctgc tgcaatccga
    12841 tgttgaccac gcgcagtggc gtcaagccga actgcagatc ccacgtgcta gcttgaacgc
    12901 catcgccttt ggcaaagacg gcttcggtct ggtggtgggc aatcgcgggc tcgtgctgcg
    12961 aaccgaagat ggcggcaaga gctggcagcg gctcaagatc atcacccgat ccttggaaaa
    13021 ccatcaaagc gcccgatgag acggcttgcc accttcattg tcagtaagcg cgccatcatt
    13081 ttaggcgcca ttgtcgtcgt gactctgtgg tttgcttacc tttcagtcaa aatcg
//
LOCUS       DASUVS010000372         7205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_813588, whole genome shotgun sequence.
ACCESSION   DASUVS010000372 DASUVS010000000
VERSION     DASUVS010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813588"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1355)
                     /locus_tag="VF182_19970"
     CDS             complement(171..1355)
                     /locus_tag="VF182_19970"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439402.1"
                     /translation="MSYRTIDDMFEPGTLIRLTDLWTPALARYRDRPAVVIDVEVLGY
                     PRSISRQENGTSLSEKYDAIYTVALMPVERAAILAREQVPVIRVISLDVVGTLPAEQE
                     SADFAAWCASEFCGAEPASVANPNTSGSGRFIGAAVLPHTIPMYEARHRPNDHRNKSL
                     DAIDQVRRHLEQWQPDVIVLASTHWMPRDGFFIDDGAQHQDGCDASYQGVEPQLFSFP
                     GDPELAALIGGLARGAGLPVRRIHRVAQEHAVWVPGSLLCQERSIPLLPCSIWWRGPR
                     EAHRRFGEIIAEAVRRLGRRAFFVASGGLSHTFDFSKPPEYVVPQGERFDRLAQEWLE
                     RGQHGRLLDLSEDDFETWNPEGRAGHLYMMRGALGKDVRGQSLCYQGSNGTGYLTMLF
                     QN"
     gene            1680..2522
                     /locus_tag="VF182_19975"
     CDS             1680..2522
                     /locus_tag="VF182_19975"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6439403.1"
                     /translation="MPDLAQAPSPSARSQADAVIHVEGVSKFFQAPSQENLGALLDID
                     LEIRHGEFVTIVGPSGCGKSTLLKLIAGFSPVSDGRIMFQGGEVRGLNTRVGYVPQES
                     KLFPWLTVEENVGFGLSPKKYSRANRERQVSYFIKLAGLSGFEKYYPAQLSGGMSKRA
                     SIIRALAYEPPVILMDEPFGPLDAQTRMVLQDELLKIWEQKRQTIVFVTHDLVEAVAL
                     ADRVVVITHRPGKIKDIIKIDMARPRNIFEIHRQEGFDDAYSRLWNIFRNELNIGPAS
                     ERTS"
     gene            2600..3376
                     /locus_tag="VF182_19980"
     CDS             2600..3376
                     /locus_tag="VF182_19980"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6439404.1"
                     /translation="MRTLTVTLIRSAIVIGFLLAWELASGRWIEPFLISSPSRIITSL
                     ITGFSSGDLLQHTWVTFMEVAIGFPIGAITGIALGYGFGRSRLLAEVFEPIIIALNGI
                     PRTALAPLFIVWLGIGLWSKVGVVFLLTFFLNFFNTYTGMRQMDQEYVDLAGLMGVRG
                     WKLTLKVIFPAISPYVFTGLRTSIPFAVIGAIVGEFVAATEGVGFFIRMSAGIFRTAD
                     VFVGIIVLMIMVIIMDKIAEAVERRALRWQTQKEHVQIQG"
     gene            3396..4451
                     /locus_tag="VF182_19985"
     CDS             3396..4451
                     /locus_tag="VF182_19985"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439405.1"
                     /translation="MKRLMKIARVMITLSAFLFASAPAKIYAQSKEMPEVIMMTAVPN
                     FAFAAIWVAEQLKYFEQEGVRVKITPASGGSPCQSAVVGRSAHFCASSSEGLVLAQVE
                     GAPLIAIQAHNRALTLSVAMRKPIVDKLGLTRESPIDARLKALLQVGTIGATSPGAVS
                     EQIFKFLVKKAGGDPSKLKFAYLGAAELPASLMNNVVDTYALSPPSAEVTEPSGKGYV
                     LIPLGKGEVQELTDYPYEVLMVRPDYLESNAKTATAVARAISRGGALFRSKPEAAKSA
                     LRSHRLFDKQRIPDDVFDLSFDMVVNAMPTWGNMNQEGWQKVVNFAGGAGIIKDPSKT
                     PSAKEGVLWTNKYVGKP"
     gene            4563..5627
                     /locus_tag="VF182_19990"
     CDS             4563..5627
                     /locus_tag="VF182_19990"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439406.1"
                     /translation="MITVFASMRPILKPALVTVLAILLAIPGKITAQQSELADVTMAT
                     AVGNLAYSAVWVAEQLKYFEEEGVRAKITVAGGGAPCQSAVVGRSTHFCASSSEGLVL
                     AKQEGASLIAVQAHNRNVTLGIAVRKAIADKHKLTRKSPLNERLKVLTQLGTIGATSP
                     GAVSEQIFKFLVRKIGGDPSKLKFAYLGGNELVPALMNNVIDALAQSPPQAESTEASG
                     KGYVLIPLGLGEVTELTNYPYLVLMSRPDWAEQNPKLVSATARAISRAGALFHTNPDQ
                     AKAALRAHRLSDSKRLDEDLFNLAYSMVVNAIPAWGNMDQEGWQKVLNFAAGAGIIKE
                     PATASAKEGVFWTNKYVGKP"
     gene            5667..6722
                     /locus_tag="VF182_19995"
     CDS             5667..6722
                     /locus_tag="VF182_19995"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439407.1"
                     /translation="MSFRKRVRQSFSALAVLTGTALCWQPSYAQQKDLGEVNILTAVS
                     NMAFSALWVAEQLKYFEQEGLRAKITPAGGGAPCQNAVVGRSAHLCASSSEGLVLAQI
                     EGAPLIAIQAHNRNLTLSIAIRKAITDKFKVTRGSPLNDRIKVLTELGTIGATSPGAV
                     SEQIFKFLVPKVQGDPSKLKFVYLGGTELPPALMNNVIDALAQSPPSAEITEAAGKGY
                     VLLPLGLGEVTELTNYPYEVLMARPDWAEQNPALATAASRAISRAGALYHSNPSAFKA
                     ALRAHRFSDKSKLEDNVFDLAYSMIAPAMPTWGNMNQEGWQKVVDFSTGAGIIKDRSK
                     APSAKEGVLWTNKYAGK"
BASE COUNT         1629 a         2011 c         1997 g         1568 t
ORIGIN      
        1 gtccgacggt tggaactttt atcattgcgc ggaagactct gtttacgggc tgctcaaaag
       61 gatctcagat acaaggcgcg cgaagttcga tgagctgagg cgtacgcttc agtacgttga
      121 agcgaatcgg gcgagcgcaa cgaagtatat gagtcctttt cagcggcctg ttaattttga
      181 aagagcatcg tcaagtatcc ggtcccattg cttccctgat agcagagcga ctgacctctg
      241 acatctttgc ctagggctcc gcgcatcata tagagatggc cggcccggcc ttcgggattc
      301 caggtttcga aatcatcttc gctcaagtcg agcagccgac cgtgctgtcc tctctcgagc
      361 cactcttggg cgagccgatc gaaacgttct ccctgtggca cgacgtattc gggcggcttg
      421 gaaaaatcga atgtatgcga gagcccgcca ctggcgacga aaaatgctct gcgaccgagt
      481 cgccgcactg cctcggcgat aatctcacca aaccggcgat gcgcttcacg cggcccgcgc
      541 caccagattg agcacggcaa aagcgggatg ctccgctcct ggcagagtaa actgcccggt
      601 acccagactg cgtgctcctg cgcgacccga tggatacgcc tgaccggtag tcccgcgccg
      661 cgcgccaggc cgccgatcag ggctgcgagc tccgggtcgc ccggaaaact gaaaagctgc
      721 ggttccacgc cctgataaga cgcgtcgcag ccatcttggt gttgcgcgcc atcatcgata
      781 aaaaagccgt ccctcggcat ccaatgagtc gatgccaaga caatcacatc gggttgccat
      841 tgctccaagt gccggcgcac ttgatcgatg gcatccagcg atttgttacg atgatcgttg
      901 gggcggtggc gcgcctcgta cattgggatc gtatgcggca acacggcagc gccgatgaag
      961 cggccgctgc cggacgtgtt cgggttggca accgacgccg gctccgcgcc gcaaaattcg
     1021 ctcgcgcacc aggcggcaaa atctgccgat tcctgttccg ctgggagcgt tcctaccaca
     1081 tccaaagaga tcacccgaat caccggaact tgctcccgcg caaggattgc cgcgcgttca
     1141 accggcatca gcgcaacagt gtagatcgcg tcgtatttct cactcaggga cgtgccattt
     1201 tcctgccggc tgatcgaccg cggataacca agcacttcga catcgataac cacggcgggc
     1261 cgatcgcgat aacgggcaag cgccggtgtc cataaatcgg tcaaacggat caaagtccct
     1321 ggttcgaaca tgtcgtcgat ggttcgataa ctcataaatc actccccggg ttactgttca
     1381 tcgctaatca acttagcaaa aaactatgaa ccttcgccat aacaaacggg gcgcgtcggg
     1441 gtgatttcca gaattcactg cgctctttat tgaaacactt cgacccctcc tgcctggtct
     1501 cgccgtttcc gccccgatgc ggacaagaaa aaagctgggg gaaaggattg agcctaatga
     1561 aagcgtcagc attctggtcg catcatcgtt ctatgaaaag caccagtacc aaagtcctac
     1621 tgcggttgac acaccagggg ttagatgaat aaataccgcg caaggtaagc aagaatctca
     1681 tgccagacct tgcacaggct ccttccccat ccgcgcgcag ccaagccgat gccgtgatcc
     1741 acgtcgaggg cgtctcgaaa ttttttcaag cgccgagcca agaaaacctc ggcgcgttat
     1801 tggatatcga tttggagatc cggcacggcg aatttgttac catcgtaggc ccaagcggct
     1861 gcggcaagtc cactttgctc aaactgatcg cgggattttc gccggtttct gacggccgga
     1921 ttatgttcca gggcggtgaa gtgcgcggtc tcaacacccg agtcggctac gtccctcagg
     1981 agagcaagct ctttccctgg ctcacggtgg aggagaacgt cggttttgga ttaagtccca
     2041 agaaatactc ccgggcaaat cgagagcggc aagtcagcta ctttatcaaa ctcgcgggcc
     2101 tttccggctt cgaaaaatac tatcccgcgc agctatcggg ggggatgagc aaacgggctt
     2161 caatcatacg cgcgcttgct tatgaaccgc cggtgatctt gatggacgag ccgttcggtc
     2221 cattggacgc ccagactcgt atggtgctgc aggatgagtt gttgaagatc tgggaacaga
     2281 agcgccagac catcgtcttt gtcacacatg acctggttga ggccgttgct ctagccgacc
     2341 gtgtagtggt tatcacgcat cgtcccggca agatcaagga tatcatcaaa atcgatatgg
     2401 cgagacctcg caacattttt gagattcacc gccaggaagg ttttgacgat gcctacagcc
     2461 gtttgtggaa tatctttcgc aacgagctaa atatcgggcc cgctagcgag cgcacttcat
     2521 gacggatcaa gtcggccaac cgcgctcagc cataactcag cccaatcgcg caaccacacg
     2581 atcaacgtcg atcttttcaa tgcgtactct gacggtaaca ctaatccgca gcgccattgt
     2641 catcggcttt cttcttgcgt gggaactcgc gtccggcaga tggatcgaac cgtttctcat
     2701 cagcagtccc agccgtatta ttacgtcgct aatcaccgga ttctcctcgg gtgacttgct
     2761 gcaacacacc tgggtgactt ttatggaagt cgccattggt tttcccatcg gcgctatcac
     2821 cggtatcgcc ctcggttatg gcttcggtcg cagccgcttg ctcgccgaag tttttgagcc
     2881 gatcatcatc gccctcaacg gcattccacg caccgcactc gcgcctctct ttatcgtctg
     2941 gttgggaatc gggctatggt cgaaagtcgg cgtggtattt ctccttacct tcttcctcaa
     3001 cttttttaac acctataccg ggatgcggca gatggatcag gagtacgtcg atctggccgg
     3061 cctcatggga gtccgcggtt ggaaattgac gttaaaagtt attttcccgg ctatctcgcc
     3121 ttatgttttt accggactcc gcacaagcat tcccttcgcg gtcatcggcg ccattgtcgg
     3181 tgaattcgtc gctgcaactg aaggagtggg gtttttcatc cgcatgtcgg ccgggatttt
     3241 cagaaccgcc gatgtgttcg tcgggatcat cgtgttgatg atcatggtca tcatcatgga
     3301 taagatcgcc gaggcagtgg agcgccgggc attacgttgg cagacgcaaa aagagcacgt
     3361 tcagatccag gggtaagatg aaaggagaac gagcaatgaa gaggctcatg aaaatcgcgc
     3421 gcgtgatgat cacgctctct gcttttttat tcgccagcgc gccggcaaaa atctatgcgc
     3481 aatcaaagga aatgcccgaa gtgattatga tgacggcggt gcccaatttc gcattcgccg
     3541 ccatctgggt tgcggagcag ctaaaatatt tcgaacagga aggtgtgcgg gtcaagatta
     3601 ccccggcttc gggaggctcg ccgtgccagt cagccgtcgt cgggcgttcg gcgcactttt
     3661 gcgcttccag ctccgaaggt ttggtattgg cgcaagttga aggagcgcca ctaatcgcca
     3721 tccaggcgca caaccgcgct ctgacattaa gcgtcgccat gcggaaaccg atcgtggata
     3781 aactgggatt gacccgggag agccccatcg acgcgcggct taaggcgttg ctccaagtgg
     3841 gcacgattgg cgccaccagc ccgggggcgg tttcggagca gattttcaag ttcctggtta
     3901 aaaaggccgg aggcgatccg tccaagctca agtttgccta cctcggcgcc gcggaactcc
     3961 cggcatcgct gatgaataat gtggtcgaca cttacgcgct ctcgccgccc tccgccgagg
     4021 tgaccgaacc aagcggcaag ggctatgtct tgattccgct cggcaaaggc gaggtgcagg
     4081 agctgacgga ttatccttac gaggtcctga tggtccggcc ggactatctc gagtctaatg
     4141 cgaaaaccgc caccgcggta gcccgcgcga tatcgcgcgg cggcgcgctc ttccgcagca
     4201 aaccggaagc ggcaaagagc gccctacgca gtcaccgtct tttcgataag cagcggatac
     4261 cggacgacgt atttgatctt tccttcgaca tggtcgtcaa tgcgatgccg acttggggca
     4321 acatgaacca ggagggttgg cagaaggtgg tcaatttcgc cggcggcgca gggatcatca
     4381 aagatccctc caaaacaccg tcggctaaag aaggcgtgct atggaccaat aagtacgtcg
     4441 gcaagccata gcccattcgt tcatcgcgcg atacgagaaa ggaatgcgga ggttgcgggc
     4501 gcagtaaata gcttgtcact tttcactctt aaagcctcgc cttctaaaga cgaggaggag
     4561 aaatgatcac cgtattcgca tcgatgcggc ctatcctcaa accagccttg gttactgtgc
     4621 tcgccatctt gttggcgatc cctggaaaaa ttaccgcaca acaaagcgaa ctcgccgatg
     4681 tgacgatggc tactgcggta ggcaacctgg cgtattcggc ggtttgggtt gccgaacagc
     4741 ttaagtactt tgaggaggaa ggtgtgcggg cgaagatcac ggttgccggc ggcggggctc
     4801 catgccagtc ggcggtcgtg ggcagatcga cacatttctg cgcctcgagc tccgaggggc
     4861 tagtgctcgc aaaacaggaa ggcgcatcgc tcattgcggt gcaggcccac aatcgtaacg
     4921 tgacgttggg cattgcggta cgtaaagcga ttgccgacaa acataaactt acccgaaaga
     4981 gcccattgaa cgagcgtctg aaagtcttga ctcaattggg caccatcggc gcgaccagtc
     5041 caggcgcggt atcggagcag atctttaagt ttctggtaag aaagatcggc ggtgatccaa
     5101 gcaaattgaa gttcgcatac ctgggcggca acgagttggt tcccgcgtta atgaacaacg
     5161 tcattgacgc cttggcgcag tccccgccgc aagccgaaag caccgaggca agcgggaaag
     5221 gctacgttct gatcccatta ggcctcggcg aagtaacaga gctgacgaat tatccctatc
     5281 tggttttgat gtctcgcccc gattgggcgg aacagaaccc taaactcgtg agcgcaaccg
     5341 cgcgggcaat ctcacgtgcc ggcgctctct tccacaccaa tcccgatcaa gccaaggccg
     5401 cgttgcgggc gcaccgacta tccgattcaa aacggctcga cgaggattta ttcaatctgg
     5461 cctattccat ggtggtcaac gcgattccgg cctggggtaa catggatcag gaagggtggc
     5521 aaaaagtgct gaactttgct gccggtgccg gaatcattaa agaaccggcc acggcatcgg
     5581 ctaaagaagg cgtgttctgg accaacaaat atgttggcaa gccatagcgt ggctgccgac
     5641 agagtttgca tgaggtgaaa ccgattatga gcttccgtaa acgcgttcgc caatcatttt
     5701 ctgcgcttgc cgtactcact ggaactgcac tttgttggca acccagttac gcccagcaga
     5761 aagatcttgg cgaagtgaat attcttactg ccgtttccaa catggcattc tccgctctct
     5821 gggttgctga acaattgaag tacttcgaac aggaaggcct gcgcgccaag atcactccgg
     5881 ccggcggcgg tgcgccatgt caaaacgcgg tagtcggccg ctccgcgcac ctctgcgctt
     5941 caagctcgga aggactcgta ctggcgcaaa tcgaaggcgc gcccctgatc gcgatccagg
     6001 cgcataaccg caatctgacg ttgagcatcg ccatccgcaa ggcgatcacc gataaattca
     6061 aagtgacgcg cggcagcccg cttaacgatc ggatcaaagt attaaccgaa ctcggcacca
     6121 tcggcgcgac cagtccgggt gccgtatcgg agcagatatt taaattcctc gtgccaaaag
     6181 tgcaaggtga tccgagcaag ctcaagttcg tttatctcgg tgggaccgag ttgcctccag
     6241 cgctgatgaa taacgtgatc gatgctctgg cgcagtctcc gccctctgcg gaaatcaccg
     6301 aggccgcagg caagggttat gtgttgcttc cgcttggcct gggcgaagtg acggaactga
     6361 cgaattatcc ttacgaagtt ctgatggcgc ggccggattg ggccgagcag aatcccgctc
     6421 tggcgaccgc cgcatcccgc gcgatttcgc gcgccggcgc gctttatcac agcaacccca
     6481 gcgcgttcaa agccgcgctg cgcgcccatc gtttttccga taaatcaaag ctggaagaca
     6541 acgtcttcga cctggcctat tcgatgatcg cgccggcaat gccaacctgg ggcaacatga
     6601 atcaggaagg ttggcaaaaa gttgtcgatt tctcgaccgg ggcgggcatc atcaaagacc
     6661 gatctaaagc gccttcggcg aaagaaggcg tgctgtggac gaacaagtat gcggggaaat
     6721 aatcgaggtt tttagtgcag ggttttggta gcggtcgact aatcattggg ctcgtgaaca
     6781 acaaattcac agcacgattc agtcattatt ccgggcgagc tggcaatatc tagcgcgatc
     6841 cggaatccag gaattctttt gaagtgctgg atgccctttt ttccacgggc atgacggcgc
     6901 gttgaatttt caacgttgtt ccgtgacggg catcacatcc ggacgcggca tgaatcaaga
     6961 ggttttttgt tcgatcaagt cggaacctaa ttgtgagccg tcggtccggc aatccagaat
     7021 gcgtgaccag ttcgagtagg tggacaattt gatgacctct acacgcaact gtttaaacag
     7081 gtatcgaatt accgtcgctc atcacgaacc atttcggcta gttcacgcct gaatcttctg
     7141 cgtacgaagg atcggcgagc ctaagtttcc gccgagagtt ttcaaatccg gatgtccgtc
     7201 actat
//
LOCUS       DASUVS010000373        11507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_376995, whole genome shotgun sequence.
ACCESSION   DASUVS010000373 DASUVS010000000
VERSION     DASUVS010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11507
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376995"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VF182_20000"
     CDS             <1..258
                     /locus_tag="VF182_20000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6439408.1"
                     /translation="GYQKAWSYSYELIILDVRMPGLLGTEIAAALKQKNPSAKIILIS
                     AFADAVLREAASNLGTPVLSKPFSPAELFQAIDNTMSEHEG"
     gene            615..956
                     /locus_tag="VF182_20005"
     CDS             615..956
                     /locus_tag="VF182_20005"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6439409.1"
                     /translation="MPEIKTRADRPLEDSSVAVGEIMNTEVESIEPNATASFAWSEMR
                     RRGIGHLVVMEYGRLRGVVSQRDFGRPTSAVIRTGRMVHDLMTPTVVSVDIARSSQLD
                     VGTTHRLTPRL"
     gene            1001..1441
                     /locus_tag="VF182_20010"
     CDS             1001..1441
                     /locus_tag="VF182_20010"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEX6439410.1"
                     /translation="MNLGRSPRRRSFPGWLPKALKRKAGRSTTTPVPAHIRVLGANLS
                     KAKREEIRRKLGTKLGKFAEANERVTVRVKDMNGPRGGIDQACTIKVVLRNLPSVVVE
                     DRSASLDMAVGAAHSGTERAVRRVLQRRRMKTIKKPQLVENNFG"
     gene            1772..2665
                     /locus_tag="VF182_20015"
     CDS             1772..2665
                     /locus_tag="VF182_20015"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6439411.1"
                     /translation="MSKLKNILAATDLSPRGDKALARAAHLAREHSARLAILYVQQER
                     ARENAKLHRIAGTIEKGLRETIAAWSLDDAAAITLVSGRPSAEIIRHAREAEADLIVA
                     GDRGANLLADLIRPATAESLVRQGDRPVLLVKHTPRGPYRRVLVPVDFSEHSRGALEY
                     ARRLAPDAEIHVLHAYEGVERQLWRTDIRKSEIMRYRRESAKQARQAMKAFLDRVDGG
                     TYKLRQGIWFGRAPQVITMVSKRLYPDLVVVGTAGRKTWPQLFRGSVAQYVMREVQCD
                     VLVVQPARTRMELDSKSRRAA"
     gene            3241..3375
                     /locus_tag="VF182_20020"
     CDS             3241..3375
                     /locus_tag="VF182_20020"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6439412.1"
                     /translation="MIRKILAPTDLSTLSKMGVRFALTTAKHFDAEVSIYHVVDADEI
                     "
     gene            4326..4766
                     /gene="rnk"
                     /locus_tag="VF182_20025"
     CDS             4326..4766
                     /gene="rnk"
                     /locus_tag="VF182_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HEX6439413.1"
                     /translation="MSIKNKSSGHHGIYITEFDKKRLKGLIKFAAERWDNKVLPYLED
                     LDGELDRAEAVKPEEIPTDVITMNSTFRLRDLDSGEEVVYTLVFPAEADSKNRKISVL
                     APIGTAVLGYRVGDTVEWEVPAGIKRLKVDEIVYQPEASREFHY"
     gene            5305..7200
                     /locus_tag="VF182_20030"
     CDS             5305..7200
                     /locus_tag="VF182_20030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEX6439414.1"
                     /translation="MENKIGAFAKIQEFPRPGPGATSLPVVTIKIYKIAGPLGLGIVR
                     KTPATALDAQRLVVRYIAMRYLVLVTDYDGTIATDGQAETAALQAIERLRMSGRRVIL
                     LTGRRIDDLVAACRRLRLFDYVVAENGAVIYEPRTREQTLLGRPPPAPFVKRLRELTE
                     NSLEVGKVVVSTSLPHRAAVLETIRQMGLELQLIFNRGAVMVLPAGVNKASGMQYALR
                     KLGFSVHEAVGVGDAENDHSFLERCECAVAVANAVPSIRELAAFTTKSEAGRGVAELI
                     EELIANDLSQMQGQLERNLVMLGLGSDGRPITVPPYGLNILIAGASGSGKSTVTAGIV
                     ERLIDRAYQVCIVDPEGDYGALPEVLTLGSPRSAITVNQALAVLEDPKMNLNVNLLGI
                     PLADRPEYFGRLFPNLQAMRTRAGRPHWIVLDEAHHMLPVEWGHVGRALPQRLGETVL
                     VTVHPEHVAPLILSLIDVVILVGPSPDKTLESFTAATEQTVTWPAGLSHQHGHVVVWF
                     SRSGKAPFSMRLLPGRVERIRHLRKYAEGNMRNRSFYFRGPARRHNLRAQNLVIFSQI
                     ADGIDEETWLYHLYRGDYSRWFRDAVNDSYLADHTQRIEQRKDLQPGETRDLIRSFIE
                     ARYTLPQ"
     gene            7270..8313
                     /locus_tag="VF182_20035"
     CDS             7270..8313
                     /locus_tag="VF182_20035"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6439415.1"
                     /translation="MKIAQISSIYLSIPPKTHGGTERIVFDLCQGLTKRGNQVELFAS
                     GDSKTDCLLQSVLPVASEDDPRSTFYLEKEFEARNTENLYRQADRFDIIHAHWPTLAP
                     YFSSLSKTPTLITYAYIEKGLHDYYRDHFPRCFPVCISRAQAKMLRDESLPVVYNGID
                     LEAIPFNDQPEDFCIIVGRMTPGKGIAEAIRIAKRANVRLVIVGQVTSHLPWSKQYFA
                     EEIQPHIDGNRVTHIGSLPHDRLMQIVARAKGFLFPSQWEEPFGLAIAESMAAGTPVL
                     AYPRGSMPELVRHGESGYLVCSEDEMVEAIYRIESLDRRRCREWVRQKFSIERMVESY
                     EMLYRRIASGARA"
     gene            8297..9361
                     /locus_tag="VF182_20040"
     CDS             8297..9361
                     /locus_tag="VF182_20040"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX6439416.1"
                     /translation="MEREPEVRLTAHASYKQWSLQRLRAAGDYCQARILSTAVHLELF
                     EWFGNRIKSASAASNRFGGTPEIWKIFLDALCAMGLLQKRAMSYENSSFILRCLCSGE
                     GLFLRSYHDAWNIWATLPDILTVGKRPKIAEPFFTDSKRSERLLQSLDHDARKIAPYL
                     IDQLPLTRSETLLDVGGGLGSFSIACCRRFSHLRATIVEHPAVAGFTSRAVKKAKIAK
                     RVKVMSVDFMKDALPRGFDVVLMSNVLHGQGAAENRALLKSAYRSLNQGGRIVLRDVL
                     MNRAGTDPDWGALFSVALLLQTPRGRCYRLDEVRDWLDAAGFSDIQGPFRSSPLAFDP
                     DSVLIAEKSRIAKDRGGLGS"
     gene            9501..10292
                     /locus_tag="VF182_20045"
     CDS             9501..10292
                     /locus_tag="VF182_20045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6439417.1"
                     /translation="MQLAGKIALVTGAGRGIGKAIAIGFADSGADIIAVSRTLPEIEE
                     TVRTVNVAGRRGLAIRADVSRFEDVERIAQQVRSHFGRLDILVNNAALRMNYLGTKES
                     YYIPFVDLTVEDWDSTIKVNLRGPFLCIRELLPLIRTNSAGSIINISAGGGKRGMAGR
                     APYCASKFGLEGLTQSLALEFGGLNIAVNALSPGKHSILTDQAKREQLGLSPAAFFMK
                     PEMMVPPAIFLALQDGHGTTGRHIEALDWVMQNGHGGIEEWKARV"
     gene            10319..10891
                     /locus_tag="VF182_20050"
     CDS             10319..10891
                     /locus_tag="VF182_20050"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX6439418.1"
                     /translation="MAIKIYKEKMEYLNKALAELPQKSDPKDYRSTPNVTINWVENCS
                     GKTTIRDQELISDENAHSTGFGKGVSPAHTFLAGFGFSHFTQWGRAAGVLDLEIDSLE
                     EQVRGWFDRRGEYLYELGYEHKGFEEITFEVKIQSKESEAKIREFIKWADRSPPHATL
                     RRAVRLIGIFHLNGQHLTTAVYHPDRTEWR"
BASE COUNT         2762 a         2888 c         3292 g         2565 t
ORIGIN      
        1 gggtatcaaa aagcgtggtc ctattcctat gagctgatta ttcttgatgt gcgtatgccg
       61 ggactcttag gcaccgagat tgccgcggcg ctaaagcaga aaaatccctc agccaaaatc
      121 atcctgatct cggccttcgc cgatgcggta ttgcgtgaag ccgcttcaaa cctcggcact
      181 ccggttctct cgaaaccatt ttcacccgcc gagttgtttc aagcgataga taacacaatg
      241 agtgagcacg aggggtaaac cacgctggcg cgctaactag tacgtgcccg cgcgccgccc
      301 cgcatgctgt tctaaccggg tgctgccaca aacccccacc ctgcgcgcat atttgcctca
      361 gcccggcagt gccgagctaa cacacggtac aaacgttgag agtactccgc gcgatgcagc
      421 cgcgctctgg gggcatcgat cttgctctag gtacatatgg cgtcagagcg cgatgaacgg
      481 ctcaatcatc gagattacaa cttcgaattg caccgatgaa gacacatagc tgtttcacct
      541 tagcgaggcg agtcgcggtg cttgcgcaac tcggtagctc ggacccacga caataactca
      601 aaagagatgt gtctatgcca gaaataaaaa cgcgcgccga tcgtccgttg gaagattcct
      661 cggtagcggt tggcgagatc atgaacaccg aagtcgaatc catcgaacct aacgcgaccg
      721 caagcttcgc ctggtcggag atgcgacggc gtggcatcgg ccatctggtg gtcatggaat
      781 acggacgatt gcgaggagtc gtttctcaac gagattttgg acggcctacg agcgctgtca
      841 ttcggacggg gcgcatggtt cacgatctga tgacgccaac ggttgtgagc gttgacattg
      901 cgcgaagcag ccaacttgat gttggaacga cacatcgact tactccccgt ctttgatggt
      961 ggcaggctcg ttggcgtcgt aagggacacg gacgtgttcg atgaacttgg gtcgcagtcc
     1021 tagacggcga tcgttcccag ggtggttacc gaaagcgctc aagcgaaagg cgggccgtag
     1081 taccacaacc ccggttcccg ctcacatccg agtcttggga gccaatctca gcaaggctaa
     1141 acgagaagag atccgtcgaa aactcggaac gaaattgggg aaatttgcag aggccaacga
     1201 acgggtaacg gtccgcgtca aggatatgaa tgggccacgt ggcggcatcg atcaggcatg
     1261 tactattaag gtcgttttga ggaatctccc cagcgtagtt gtcgaagacc gatcagcgtc
     1321 gctcgatatg gccgtcggcg ctgctcactc cgggacggag cgggctgtgc gccgggtttt
     1381 gcaacgcagg cgcatgaaaa caatcaaaaa accgcaactg gtcgagaaca atttcggata
     1441 aagcaaataa ttaccggaaa ctaatcgcaa ggcaggcttc aataacatct tagattggaa
     1501 ttatcagcgt gcttagacgc tggttatggt cgggtaatct gcatacggta ggttcgtcca
     1561 acgagacgct accaagaaga agccatcaag tcggccagcc tgaccgcctt tttggatcac
     1621 gcatgcgcga acgtgtgatt tgcagccgct ggctaaaccc tgccatttga gccgaatccg
     1681 cagcgcaact ctgattgcgc tgtcgtgctt gatggctgtg ccgatatcga agcgcttagt
     1741 gagcattagc ttgaggccat aggtatcaat catgagcaag ttgaaaaata tactggccgc
     1801 caccgatttg tcgccacgtg gtgataaagc gttggcacgc gcggcgcatc tcgccagaga
     1861 acacagcgca agattggcaa tcctatatgt gcagcaagag cgcgccagag aaaatgcaaa
     1921 attacaccga atcgctggca cgatcgagaa aggcctgcgc gaaacgatag cagcatggtc
     1981 cctcgatgat gcggctgcga taacgctggt cagcgggagg ccgtcagcgg aaattattcg
     2041 gcacgcccgc gaggcggagg ctgacttgat tgtcgctggc gatcgcggag ccaatctttt
     2101 agcggatcta attcgaccgg caaccgctga aagcttggtc cgccagggag atcgccccgt
     2161 attgctggta aaacacactc cgcgtggtcc ctaccggcgg gtgctggtgc cggtggattt
     2221 ctcggaacat tcccggggcg cgctggaata tgcgcgacgg cttgcgccgg acgcagaaat
     2281 ccatgttctg catgcctacg agggagtaga gcgccaactc tggagaacag atataagaaa
     2341 atcggaaatc atgcggtatc ggcgtgagtc ggcgaagcaa gcgcggcaag caatgaaagc
     2401 ttttctggat cgcgtcgacg gtggaactta caagctgcgg caaggaatat ggtttggacg
     2461 agcaccgcaa gtcatcacca tggtttcgaa acgtttgtac ccggacctgg tggtcgtcgg
     2521 gactgccggc cgcaagactt ggccgcagct gtttaggggg agcgtggccc aatacgtcat
     2581 gcgtgaagtt cagtgcgacg tgctggtggt gcaaccggcg cgcacccgta tggagctgga
     2641 ttcaaaaagt cggcgcgcgg cttgaaccgg gagcaggagc gacggcgcgt ctacgctttg
     2701 gttgcttgat accgcggcct ttgatgctca agatgcctgt tgcgcaatca atttgctgtc
     2761 gcccaaatgt gtgcgatgcg cggcaccacg cctcagtatt aggcggaatg cgatcaagca
     2821 gctcgttcgg ccttacactt ccgcttgtgc agatgcgcta ttcgagcgct gacgatcaga
     2881 tatccgtgat cgaagctgag caatcacacg cggtttccga gtagaggccc agcgggtctt
     2941 atttcgaacc ggttttcggt ggcagataag ataaccagat gagaactgcc ggcggacctt
     3001 cctgtacgag cgctggtcga tgcgcggcgt cttggcatct aagttgctct actaatacac
     3061 gtcacccgaa gacaatcgaa tccaagccag agtctcccag atcgagcaac ggctagagaa
     3121 tccttcgcga acttacgccg gcagaaccga tcaggaaata tacgaaacga atgagtcaga
     3181 tgtccggtcg ctcgtattca ccaagcgacg cggtctattt tgctatcagg aggtattatc
     3241 atgatcagaa aaattttggc gccaaccgat ctttcaacac tctcaaagat gggcgtgcgc
     3301 tttgctctca ccactgcgaa acactttgac gctgaagtca gcatctatca cgtcgtagac
     3361 gcagacgaga tttgaaaact aggtgatagc ctcacggggg cgcactttca tcgaaagcgc
     3421 gttgcctgac gtgctggaaa cttacttgca cacttacgaa gtcagcctgg cacagttcgt
     3481 agggcaaaac ttttcagatc taacttccag cggctaaggt cgaggaaaag gtagaacttg
     3541 gcagtccaag caagaatatt gtcgacgcac gcaaagacgg agggagtcga cttgatcatc
     3601 atggctactc gcgggaagag tggactgtcg cagttatttt taggaagcgt aaccgagcag
     3661 gtcatccgca acgcgcgtgt ccggtactgg ccattccccc ttttccagcg acggtcggag
     3721 agtcggataa cgagaagcgt tttgagccgg tgaaagtctc cggctatcag gcacggtcca
     3781 agtcacgctc ccgccggcgc catccctata tggccgtagg cccggtcgat atctggaggg
     3841 cacgaacaag agaaaaagat aaaatgattt gaagatagga aaaacgggaa atgtttgtca
     3901 acgtcgctaa ggtgaacaag ctgacaagag gaaaggcgaa actcattcgt gtcgagggcc
     3961 gttctattgc agtcttcaat gctgatggag cgttctatgc tctcgaagat acgtgtccct
     4021 ttgacggcgc ctctctctcg aatggatggg tcgctggaag cgtagtcgaa tgccggggga
     4081 cagggccaaa ttttttctcc cgaatggcga gtgtctctgg caattgggag ggaaaaatat
     4141 caggagcttc agggtactgg ccgatacgga acacgtcttt gtcgagttgc ggccaaggtc
     4201 ggcgcctgaa atgacccaag acgagggtgc agccggcttg cggcccgttg cgatggccgg
     4261 tggccgactc tgataagagt cggatgctga tcacaatcaa aagaataaaa ataatggaga
     4321 cggttatgtc gattaaaaac aaatccagcg gtcatcatgg aatttatatc actgaattcg
     4381 ataagaagcg gcttaaagga ctcatcaaat ttgccgcgga gcgctgggac aacaaggtct
     4441 taccgtatct ggaggacttg gacggagagc tcgatcgagc cgaagccgtt aagccggagg
     4501 aaatcccgac tgatgtcatt accatgaact cgacgtttcg cttgagggac ttggatagtg
     4561 gagaagaggt cgtttacacc ttggtttttc cggctgaggc cgattccaag aaccgtaaga
     4621 tttcagtcct ggcgccgatc gggaccgcag tgttgggcta tcgagttggc gatacggtcg
     4681 agtgggaggt gccggctggg atcaaaaggt tgaaagtcga cgagatcgtc tatcagccgg
     4741 aagcgtcgcg cgaatttcat tactaactct ccgcaataca gaggaaactc tacttgtgcg
     4801 aggtcgaaag aaagactaga gaaaaggcga cgacgtttat gggagaaaca ctgcatgaca
     4861 tcggaaataa cagagttaat gaagattggt ctttatcttc tcttaagttt cggcggcgga
     4921 ataatgatag cagtgttagg cattcttttg atcattgtaa tcggcgccgt gggcgcttgg
     4981 ttggtccata tactgcttcg tccgagaagc ggacaaatcc gcttgtgaca aacagaaaag
     5041 gatcgacaga gaagtcagga agatcaatcc gtgcatttcg catcgtccgg atgtttgaag
     5101 tcagcccaga ttagagactc attccgaaga tgaaatggaa tcggtagctc aattatttaa
     5161 tgaaagagaa aggatccact tgaatatgat acttttaaat tctgtcttta tggtatttgg
     5221 tttgttttcg tcggttgttt tgcctagcgg catcattgcc acgctgtgtc tagcgattgc
     5281 cgcgctggtg attagtatcg gcgaatggaa aacaagattg gggcttttgc caaaattcaa
     5341 gagtttcccc gccccggccc aggagcgaca tcgctaccgg tcgtcaccat caaaatttac
     5401 aagatagcgg gccctttggg gttagggatc gtccggaaaa cgcccgccac cgcactcgat
     5461 gcacagcggt tagtggtgag atatattgcg atgcgctatt tggttctggt gactgactat
     5521 gacggcacga ttgcgaccga cggtcaggcg gaaacagctg ctctgcaagc catcgaacgg
     5581 cttcgaatgt ccgggcgccg cgttattctg ctaaccggca ggcgtatcga tgaccttgta
     5641 gcggcctgtc ggcggctgcg cctattcgat tacgttgtgg cagagaatgg ggcagtgatc
     5701 tacgagcccc gaacgcgcga gcagacgctc ctcggcagac ctccgcccgc accgtttgta
     5761 aagcgattaa gagagctgac cgaaaattca ctcgaggtag gaaaagtggt agtcagcacc
     5821 tcactacctc atcgcgccgc ggtgctcgag acgatccggc aaatgggctt ggagctccag
     5881 cttatcttca atcgtggcgc cgttatggtt ctacccgccg gcgtcaacaa ggcaagcggt
     5941 atgcaatacg cattgcgcaa gctcggcttc tcggtgcatg aagcagtggg agtcggtgat
     6001 gccgagaacg atcactcgtt tcttgaacgt tgcgaatgcg cggtagcagt tgctaacgcg
     6061 gtaccgtcga ttcgcgagct tgccgccttt accacgaaaa gcgaggctgg gcggggagtt
     6121 gccgaactta tcgaagagct gatcgcaaat gacctgtcac agatgcaggg tcagctcgaa
     6181 agaaacctgg tgatgctcgg tttaggttcg gatggaagac cgatcaccgt ccctccatac
     6241 ggtctgaaca tcttgattgc cggcgcttcg gggagcggca agtccacagt gacggcgggc
     6301 atcgtggagc gactcatcga ccgagcttac caggtctgta tcgttgatcc tgagggtgat
     6361 tacggtgctt tgccggaagt cctgacgctg ggaagtccac gttctgcaat cactgtaaac
     6421 caggcgctcg ccgtcctcga agatcccaag atgaatttga atgtgaattt gttgggaatt
     6481 ccactcgcgg atcggccgga atacttcgga cgtctattcc caaacctgca agctatgagg
     6541 acgcgagccg gccggccgca ttggatcgtc ctcgacgagg cgcatcatat gttgccggtt
     6601 gagtggggac atgtaggtcg agccttgccg cagagactcg gagaaacggt gttggtgacg
     6661 gtgcatcccg aacatgttgc gccgttgatc ttgtcgctga tcgatgtggt gattctggtg
     6721 ggaccctccc cagacaaaac tttggaatcg tttactgctg cgacggaaca gacagttacg
     6781 tggcccgcgg gcttgtcgca ccaacacggg catgtggtcg tgtggttttc gcgcagtgga
     6841 aaagcaccct tttcgatgcg attattgccg ggtcgcgttg agcgcattcg tcatctccga
     6901 aagtatgctg agggtaacat gcggaatcgc agcttttact ttcgcggacc ggctcggcgt
     6961 cataatctca gggcgcagaa ccttgtgatt ttctcgcaga ttgccgatgg tatcgacgaa
     7021 gaaacctggt tgtaccactt gtacagaggc gactactcac ggtggtttcg cgatgctgtg
     7081 aacgactcgt acctcgcgga tcatacgcag cggattgagc aaagaaaaga tttgcaaccc
     7141 ggcgagacca gagatcttat acggagcttc atcgaagctc gttacacatt gccgcagtag
     7201 cgtcattgcg tttttgtccg tcactttgag gtttacgatg gcgtaagcca actccgctag
     7261 ggccagagaa tgaaaatcgc acagatctcg agcatctatc tgtcgatccc accgaagact
     7321 cacggaggaa ccgagcggat tgtttttgat ctctgccagg gcctcaccaa gcgcggcaat
     7381 caagtggagc tgtttgcttc gggagattct aagaccgatt gtctcctgca atctgtgctg
     7441 cccgtagcca gtgaagatga tcctcggtcg acattttatt tggaaaaaga atttgaggcg
     7501 cgcaataccg agaatctcta ccgtcaagcc gaccgcttcg atatcattca cgcccactgg
     7561 cctacgttag cgccttactt ctcgtctctt tcaaagacgc cgacgttgat tacctatgca
     7621 tacattgaga aagggcttca tgattactat cgggatcact ttccccgctg ctttccggtt
     7681 tgcatcagtc gggcgcaagc caaaatgctg cgggacgaat ccctgccggt ggtttataac
     7741 gggatcgacc tcgaggcaat tccattcaat gatcagccgg aagatttttg tattattgtc
     7801 gggcgaatga cgccggggaa aggtattgca gaggccatcc gcattgccaa aagagccaac
     7861 gtccggctcg tgatcgttgg ccaggtcacg tcgcatctgc catggagcaa acagtatttc
     7921 gccgaagaga ttcaaccaca catcgacggg aatcgggtga cgcatatcgg cagcttaccg
     7981 catgatcggc ttatgcagat cgtggcaagg gccaagggct ttctcttccc ttctcagtgg
     8041 gaggagccgt ttggcctcgc catagccgaa tcgatggcgg caggaactcc ggtgttggcg
     8101 tacccgcgcg gctccatgcc ggagctggtg cgacacggtg agagcggata tctggtgtgc
     8161 agcgaggacg aaatggttga agcgatttac cggatcgaga gcctggatcg aagacgttgc
     8221 agagagtggg ttcgacagaa gttttccatt gagagaatgg tagagagtta tgagatgctt
     8281 taccggcgga tcgccagtgg agcgagagcc tgaggtacga ctcacggcac acgccagcta
     8341 caaacaatgg agtctgcaaa ggctccgcgc tgctggcgac tactgccagg cccggatcct
     8401 gagcactgcg gttcacctgg agctcttcga gtggttcgga aatagaatca agagcgcaag
     8461 tgccgcgagc aaccgttttg gtggaactcc ggaaatatgg aagatatttc tcgatgcgct
     8521 ctgtgcgatg ggactactcc aaaagcgagc gatgagttat gagaattcct ctttcatact
     8581 gcgctgtctc tgttctggcg agggactttt tctccggtct tatcatgacg cctggaacat
     8641 ttgggcaacg cttcctgata tcctgaccgt cgggaaacga cccaaaatag ccgaaccgtt
     8701 ctttacggat tcaaagcgaa gtgaacgatt gcttcaatcc ctcgatcacg acgcccggaa
     8761 aattgcgcct tacttgattg accagctgcc attgactcgt tcggaaaccc ttctcgatgt
     8821 aggcggcggt ttaggaagct tcagtattgc atgttgtcgc cgattctctc atcttcgagc
     8881 gaccatcgtg gaacaccccg cagtggcagg ttttacctct cgtgccgtga agaaagcaaa
     8941 aatcgcaaaa cgggtaaagg tgatgagcgt ggattttatg aaagatgcat tgccccgcgg
     9001 cttcgatgta gtactcatgt ctaatgtgct tcatggacaa ggggcggcgg aaaatcgtgc
     9061 attgctaaag agcgcctatc gctctttgaa ccagggaggg cggattgtct tacgggatgt
     9121 cctgatgaac cgggctggga cggatccgga ttggggcgcg ctgttttccg ttgcgcttct
     9181 tttgcagaca ccgaggggtc gctgttacag gctggatgaa gtgcgcgact ggcttgacgc
     9241 ggccggattc tcggacatcc agggaccatt tcggtcaagc cctttagcct ttgatcccga
     9301 ttccgtgttg atcgctgaga aaagcagaat agccaaggat cgaggcgggc taggatcatg
     9361 agcttagccg taaagaacag atcgaggggc tacaaccccg ttgtctcgac gaagcagaac
     9421 cgccgcgagt ttgtcgacga tttgacctca agggaggttt gggaaggaat gagcgagctg
     9481 atgttctcgc aatttgaatc atgcaacttg ccggaaagat agccctagta accggcgcgg
     9541 gtcgtggaat cggcaaagcc atcgcgatcg gcttcgctga ttctggagcc gacattatcg
     9601 ccgtttcccg aaccttgcct gaaatcgaag agacggtgcg cacggtcaac gttgccggca
     9661 gacgagggct cgcgatccgg gccgacgtct ctcgcttcga ggacgttgaa cgcatcgcgc
     9721 aacaagtccg aagccacttc ggccggctcg atatcttggt taacaatgcc gcattacgga
     9781 tgaattatct gggaacgaaa gagagttact acattccgtt cgttgatctc acggtggagg
     9841 attgggacag caccatcaaa gtcaacctgc gcggcccatt tctgtgcatt agggagctcc
     9901 tgccgctgat tcgcaccaac agcgcaggaa gcatcatcaa catttctgcc ggcggcggca
     9961 aaagaggtat ggctggacga gcaccctatt gcgcttcaaa gttcggtctg gagggactga
    10021 cacaatcgct ggcgctggag tttgggggtt taaacatcgc tgtcaatgcg ctgtcacccg
    10081 gcaagcattc aatcttgact gaccaggcca aaagggagca attaggactc tccccggctg
    10141 cctttttcat gaaaccggag atgatggttc cgccagcgat ttttctggcg cttcaagacg
    10201 gccatggcac caccggccgg catatcgaag cactggattg ggtaatgcag aatgggcacg
    10261 gtggaatcga ggaatggaaa gctagagttt agtgccaagt cgactggagg aagcaaccat
    10321 ggcaatcaaa atctataaag agaaaatgga atatttgaac aaggcgttgg ccgagttgcc
    10381 gcaaaaatca gatccaaaag actatcgctc gacaccgaat gtgaccatta actgggtaga
    10441 gaactgctcg ggcaaaacta cgatcagaga tcaggagctg atttccgacg agaatgccca
    10501 cagcaccgga ttcggtaaag gcgtcagccc agctcacact tttctggccg gctttggctt
    10561 tagtcatttc acccagtggg gccgggcggc gggtgtgcta gatctcgaga tcgattctct
    10621 ggaagagcag gtgcgcggct ggttcgatcg aaggggcgaa tatctttacg agctcggtta
    10681 tgagcataag ggcttcgaag aaattacctt tgaagtcaag atccaaagta aagagagcga
    10741 agcgaagatc cgggagttca tcaagtgggc cgaccgctcg ccgccgcacg caaccctccg
    10801 tcgcgccgtt cgcttgatcg gcatctttca tctaaacggg caacatctta cgacagcggt
    10861 ctatcatccg gacaggactg agtggcgttg agctggtgaa gttaagtcac gtgaatgttg
    10921 ggtgttagcc ggttgcgact gcgctggttt tctcttagcc cacaggtccg ttaaacgtcg
    10981 ccgattcatc ttaggcaaca acaacataga gcaattctca aagtacgtta tgcaggagcc
    11041 caaaagaagc gcgccgagac taaagagaac ctccgcaagt gccaacgaac aacatcatca
    11101 acagatagag agtagccgag gtcaaatagc cacaaaaggg tgggatcatc ttttagttcc
    11161 tcctgacgca acttgttcca agcgtagtga gcaacgattt ctgtgggcgc aaatcgtcac
    11221 ctgttaggta taccgggctt ttaagcggat tgaacagctc taattcgccc gctactctac
    11281 ttacatggcg gtctatctgg ctcgcaactg tccccgctgc cgtaattact ggggcgtcac
    11341 aattgctaaa cccgacggca gcgggtcaca accggttcat ggcacgtgtg gcaggtgcgg
    11401 atacgaaatc tcgtgggcga tcgtcagatc taagtcttca tccaaatcag cgcactcact
    11461 gccgctgcga gaggaactga tgcatgaatg agttgatcgc cattcat
//
LOCUS       DASUVS010000374         5581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1470952, whole genome shotgun sequence.
ACCESSION   DASUVS010000374 DASUVS010000000
VERSION     DASUVS010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470952"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VF182_20055"
     CDS             complement(<1..429)
                     /locus_tag="VF182_20055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439419.1"
                     /translation="MNPKLTRWLLGLAAGLILILPAAFLYNRNSSPQHSAQTEPFNTV
                     EAFLKNSYARDFKKAYRYISLGDRQLKAERVYIREKGSFSGFPLAITRKLAKFIELQI
                     LQSSRQQDGRMRVKVAAKYPDANSLSAIVLDWDEERLNALP"
     gene            complement(517..1413)
                     /locus_tag="VF182_20060"
     CDS             complement(517..1413)
                     /locus_tag="VF182_20060"
                     /inference="COORDINATES: protein motif:HMM:NF016337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX6439420.1"
                     /translation="MKPKHSTGSSRGGLFAVALLTLFSLNPASAVAGEEQDALSTLDR
                     YVRATYARDYEEAYSHIASSDQRLKDRASYVRDRGAFSGFTLEIARVLASYIELSSLL
                     TQIDGGHARINIKFQVPDAAKLGPLLHDWDSDKLEALPSTGRKALLARIDKLRRDQAI
                     EMIGGEDVFQLIKEGDSWKLVLNWASPVNVGFQTSIPTSVPIQARLRHSDVITHPGEI
                     FKVVLKVKNTGQEQLLARIGHLVDPYEVRDYLDLVDCGFLLPVRLAPGKEEEFVSTYL
                     LRRNLPEGVRQLNVTYAVAVSQ"
     gene            complement(1466..2047)
                     /locus_tag="VF182_20065"
     CDS             complement(1466..2047)
                     /locus_tag="VF182_20065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015144502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6439421.1"
                     /translation="MKPLHRLSLAAILIVFTLSSAEYARSGIFTGTARAQDLESKKIV
                     PFVPTPQDVVDKMLELAGVKKGDVVYDLGSGDGRIVITAAKKGARAVGFDVDGALVRE
                     SRENIRKAGLENLAEIKQQDILTVDLSPATVVTMYLLPDVNLKLKPNLLKQLKPGSRV
                     VSHAFDMGDWKPDKTERVNGRTIYLWSIPAKAP"
     gene            complement(2135..2758)
                     /locus_tag="VF182_20070"
     CDS             complement(2135..2758)
                     /locus_tag="VF182_20070"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX6439422.1"
                     /translation="MRLKLNSSCFVTLLVLTAGLLGSTVAAHEPTKTKTEGSRRVTSN
                     VKISPFTLIDQEGNPFDTENKLRGKIVAINFIYTTCSDVCPLFTVEFARLQQALRSQT
                     QVNSFLVSITTDPEIDSPKVLKAYARRFGADFQNWAFLTGDDSRLKSVWKGFGVQVIR
                     RGRGLVQHTNLTTLIDAHGIRRINFLGPQWQSGDFLKDMRALSASPP"
     gene            complement(2736..3656)
                     /locus_tag="VF182_20075"
     CDS             complement(2736..3656)
                     /locus_tag="VF182_20075"
                     /inference="COORDINATES: protein motif:HMM:NF016337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX6439423.1"
                     /translation="MIPAFLPRDLIVLRKILLVLVLFASTALMTFYLHNRSREQITAN
                     APLAVIQSYLKATYARDYRTAYAHLSRPDRTVRDEKSYVAGQVEYTGFTARLAGMLAG
                     FMDLKLTEESSDGPRTKIKVEYSVPAPEDVSSLVWDWNAEKLNSLSTAEQARLLDAVA
                     ARQRIGNLTRIQGHQTFELIREGNGWKIFQDWGAGTRVKVHTILAGRSDVEIKLLQNE
                     IITSGQEPFQVNLAIKNRGSQPVVLAMRHVIEPFQAADYLEMIQCGLERAVVVEPGTQ
                     KEFSMAYLLDPTVRNNFQDLALTYAFEAKQ"
     gene            complement(3698..4138)
                     /locus_tag="VF182_20080"
     CDS             complement(3698..4138)
                     /locus_tag="VF182_20080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439424.1"
                     /translation="MQQSIYPTLRALIASAMIMTNLFLGGESIAAQSEHHHQDLHTTP
                     PEKTSGGAKLLPADGASVKILSPASDQIFQNDRIPLTFKLAKGKRGHHVHAYVDGELM
                     GMFDGQSGTLTGIKPGKHVLELRVVAEDHQSELNAVDRVSFSVK"
     gene            complement(4209..4748)
                     /locus_tag="VF182_20085"
     CDS             complement(4209..4748)
                     /locus_tag="VF182_20085"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX6439425.1"
                     /translation="MKLIWLRLLALGQISLAAALITAAEQPKHLHPVRQNSAPEIGAA
                     AVPFELKTLNGKPIDLSTFRGKPLVLNFFASWCDPCREEMPLINDLAAKAPAIGYSVL
                     AVAVEDSRSAVTQFAKESKLIFPIALDLNSTVKRSYRIFGPPATFFIDREGIIRDIII
                     GPLTAERASTALTHAGIRR"
     gene            complement(4745..>5581)
                     /locus_tag="VF182_20090"
     CDS             complement(4745..>5581)
                     /locus_tag="VF182_20090"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX6439426.1"
                     /translation="IENGRGAFTDARTVNEIKDTARFPIIEFAPDGNLMLAWIDRRID
                     NPRPRQLYMMRLSPEGKALTSNYSVGQGLCECCKLGLAFADGGKTVYIVDRQVDDNQI
                     RNHVVRKSIDGGKTFAAPVLISNDGWQVPSCPHSGPSIGRDSRGKLHVTWFTLGRSEK
                     EAGIYYSLSSDGGNTFAPRRLVHANTAPEILYTTLAVGDDDTIYLAWSNLDRNKRAQI
                     FLRSLSADGQSWSAIEQVSHAPGNASRPVLAVLKNRLNVAWTETDGETSKAVLRNAMV
                     TK"
BASE COUNT         1244 a         1471 c         1481 g         1385 t
ORIGIN      
        1 gggcaaagca ttgagccgct cctcgtccca atccaacacg atggccgaca agctattcgc
       61 gtcggggtat ttagctgcaa ctttgactcg cattcgaccg tcttgttgtc gcgagctttg
      121 caagatttgc agttcgataa attttgccag cttgcgtgtt atggcgagcg gaaagccgct
      181 gaacgatcct ttttcgcgaa tgtagacgcg ctcggccttg agctgccgat cgccgaggga
      241 tatgtaccga tatgccttct tgaaatcgcg cgcgtaggaa ttcttgagaa aagcctcgac
      301 ggtgttgaac ggctccgttt gtgcgctgtg ctgcggcgag ctattcctgt tatataaaaa
      361 agcggccggc aggatcagta tcaaacccgc cgccaggccg agaagccaac gtgtcaattt
      421 tgggttcatc cgatggttct tgatgaatca agtcctccat ttggtttact ctgcgttaga
      481 agttggagcc gggaatcggg taacgaggcg tcttagttac tggcttacgg cgaccgcata
      541 cgtgacgttt agctgacgga ctccctcggg cagatttctt cggagaagat acgtgctgac
      601 gaattcttct tctttgccag gcgctaatcg cactggcaga agaaaaccgc agtcgacaag
      661 gtcgagataa tcgcgaactt cataaggatc gactaagtgg ccgatgcgag cgagaagctg
      721 ttcctgaccg gtgtttttca ccttgagcac gactttgaag atttcaccgg ggtgagttat
      781 cacatcggaa tgtcggagcc gcgcttggat tggaactgat gtcggtatcg aggtttgaaa
      841 gccgacattc accggcgatg cccaatttag aacgagtttc caggaatccc cctctttgat
      901 caattggaaa acatcctcgc cgccgatcat ctcaatcgct tgatctcgac gcagtttgtc
      961 gatccgcgcc agcagcgctt tccgcccggt gcttggaagc gcctctagct tgtcgctgtc
     1021 ccagtcgtgt agcagcggac ctaattttgc cgcatccggc acttggaatt tgatgtttat
     1081 tctggcgtgt ccgccatcga tttgagtcag gagcgaactc agctctatgt agctggcgag
     1141 tactctggcg atctcgagtg tgaatccgct aaacgcgcct cggtctctga catagctggc
     1201 gcgatccttc aggcgttggt ccgaagaggc aatatgcgaa taagcttcct catagtccct
     1261 ggcgtaggtc gcccgaacgt agcggtccaa cgtgctgagc gcgtcctgtt cttccccagc
     1321 aacagcggaa gctggattca ggctaaatag ggtcagaagc gcgaccgcga acaaaccccc
     1381 acgggagctt ccagtcgagt gctttggttt catgcgatcc gtaattgcca agcaaacggc
     1441 acaacggtgc caaaggactt catcttcagg gagctttggc tggaatgctc caaagataga
     1501 tcgtgcgacc gttgactcgc tcggttttgt cgggtttcca atcacccata tcgaacgcat
     1561 gagagaccac ccgggaaccc ggtttaagtt gtttcaggag attcggttta agcttcaaat
     1621 taacatccgg cagaagatac atcgtcacca ctgttgccgg cgagagatcc accgttagga
     1681 tgtcctgttg cttgatctcg gccagatttt ccaaacccgc tttgcgaatg ttctcgcgcg
     1741 actctctgac cagagctccg tcgacgtcga agccgaccgc gcgggcgccc ttcttggcgg
     1801 cggtaatgac gattctgccg tcgccggagc caagatcgta aactacatcg ccttttttta
     1861 ctccggcgag ttcaagcatt ttgtcgacga cgtcttgcgg cgtcggtaca aacgggacga
     1921 ttttcttaga ttcaaggtcc tgagcgcgag cagtgccggt gaaaattccg gaccgcgcat
     1981 actcggcgga cgagagggta aacacgatca ggattgcagc caatgacaat cgatggagcg
     2041 gtttcatcct ttatctcctc gttctggtaa gcaatgatat ccggaacaat taaccataaa
     2101 aaaaatctag ccgctactat atcgctaagt agatctaagg cgggctcgcg ctcaacgcgc
     2161 gcatgtcttt caaaaaatcg ccagactgcc actgcgggcc tagaaaattg atcctgcgaa
     2221 tgccatgggc gtcgatcaag gtcgttaaat tagtgtgttg caccaggcca cggcctcgtc
     2281 ggatgacctg aacaccgaat cctttccaga cacttttcag ccgtgaatcg tcgccggtta
     2341 ggaatgccca gttctgaaaa tcggcgccga atcgccgtgc atacgctttc agtactttcg
     2401 gtgaatcaat ttccggatca gtggtaatgc tcacgagaaa tgaattcact tgagtttggc
     2461 ttcggagcgc ttgttggagc cgggcaaatt ctacggtgaa tagcgggcaa acgtcggagc
     2521 aggtggtata aatgaaattg atcgcgacga ttttgccccg cagtttgttc tccgtgtcaa
     2581 acggattacc ttcttgatct atcaaggtga agggagaaat tttcacattc gaggtgaccc
     2641 ggcgggaccc ttctgttttt gtcttagtcg gctcatgggc cgcgacggtg ctcccaagca
     2701 aaccggccgt aaggacgagt agggttacga aacagctact gtttagcttc aaacgcatag
     2761 gtgagcgcca gatcttgaaa gttgttccga actgttgggt cgagcagata tgccatcgag
     2821 aactctttct gggttccggg ttccaccacg acggcgcgct cgaggccgca ctggatcatc
     2881 tccaaataat cggcggcttg aaacggttcg atcacatggc gcatggctag aaccacgggt
     2941 tggctgccgc ggttcttgat cgccaaattg acctgaaaag gctcttggcc gcttgtgata
     3001 atttcgttct gcaaaagttt gatctcaacg tctgatcttc cggccaagat cgtatggact
     3061 ttgaccctgg ttccggcgcc ccaatcctga aagattttcc atccatttcc ctctcgaatg
     3121 agctcgaatg tctggtgtcc ctgaattcgt gtcagattgc caattcgctg gcgcgccgcc
     3181 acggcatcaa gcaagcgcgc ctgctccgcg gtcgagagag aattgagctt ctctgcattc
     3241 cagtcccaga ccagcgatga aacatcttcg ggcgctggaa cagagtattc gactttgatt
     3301 tttgtccggg gcccgtccga tgactcttcg gtgagtttaa gatccataaa accagcgagc
     3361 atcccagcga gccgagccgt aaagccggta tattcaactt gccctgcaac atagctcttt
     3421 tcatcgcgaa ccgtccggtc tggccttgaa agatgcgcat aggcggttcg atagtcgcgc
     3481 gcgtaagtcg ctttaagata ggactggatt accgccagag gagcattcgc cgtgatttgt
     3541 tcgcggctgc ggttatgtaa ataaaaggtc attagtgccg tcgaagcaaa caggaccaac
     3601 acgagaagaa tcttgcgcaa gacgattaga tccctgggca agaaagctgg gatcattctt
     3661 ggccgcattt ttgatctcat tcggaacact cgagattcta tttgacagaa aatgaaactc
     3721 gatcgacggc gttcaactca ctttgatgat cctcggcgac aacgcgtagt tccaacacgt
     3781 gttttcccgg cttaatcccg gtgagcgtcc ctgattggcc gtcgaacatt cccatcagtt
     3841 cgccatccac gtaggcatgc acatgatggc cccgtttgcc cttagcgagt ttaaatgtga
     3901 gaggaattcg atcgttctga aaaatctggt cgcttgcagg cgacaaaatc tttacgctgg
     3961 cgccgtccgc cggtaatagc ttagcgcctc cgctggtttt ttctggtgga gtggtatgga
     4021 ggtcttgatg atgatgctct gactgggcgg caatggattc accacccaaa aaaagatttg
     4081 tcatgatcat cgcgctggca atgagcgctc ttaacgtagg gtaaatagat tgttgcattg
     4141 acatctcctt gggcttggcc gtcgggcgac ccttgagaat ccctgcttcc gatttggagc
     4201 cgacggaatc atcttcgtat accggcatgc gtgagcgctg tcgacgctcg ttccgccgta
     4261 agagggccga taatgatgtc gcggatgatt ccctcccgat caataaaaaa ggtcgccggc
     4321 gggccaaaga tccgatagga ccgcttgacc gtactattca agtccagtgc gatagggaaa
     4381 atcagtttag actcttttgc gaattgtgtc accgcagagc ggctatcctc aaccgccacc
     4441 gccaagacgc tataaccaat tgcgggcgcc ttggccgcta gatcattgat aagcggcatc
     4501 tcctcccggc atggatcgca ccagctagcg aagaaattca gcaccaatgg cttgccgcgg
     4561 aaggtgctca aatcgatcgg ttttccgttc agtgttttta gttcaaaggg caccgccgca
     4621 gcgccgatct cgggcgctga gttttgccta acggggtgaa ggtgctttgg ttgttcagcc
     4681 gcggttatca gtgcagccgc taacgatatt tggcctaggg cgagcaatct cagccaaatt
     4741 agtttcattt cgtcaccatt gcgtttcgaa gaacagcctt tgatgtttcg ccgtcggttt
     4801 cagtccaggc gacgttcaac cggtttttta aaaccgccaa gaccggccgg cttgcattgc
     4861 ccggggcatg acttacttgc tcaatagcgc tccaggattg gccgtctgcc gataacgagc
     4921 gcaggaaaat ctgcgctctt ttgttgcggt ctagattgct ccaagccaga tagattgtat
     4981 catcgtcgcc caccgccagt gtcgtataga gaatttccgg agccgtattg gcatgcacca
     5041 gccggcgggg cgcgaaagtg ttgcctccat cactcgagag gctgtaataa atcccagcct
     5101 ctttttccga acgcccgagc gtgaaccatg tcacgtggag cttgccgcga ctgtcccgtc
     5161 cgatgctcgg gccagaatgc gggcacgatg gaacttgcca gccatcgttg ctgatcaaca
     5221 ccggcgcggc gaaggttttg ccgccatcta tcgacttgcg cacgacatga ttgcgaattt
     5281 gattatcatc cacttgacga tcgactatat aaaccgtctt gccaccgtct gcgaaggcta
     5341 agcctagctt acagcactcg cataatcctt ggcctaccga gtagttactt gtaagagctt
     5401 taccttcagg actcagccgc atcatataca gttgacgggg cctgggattg tcgatgcggc
     5461 ggtcaatcca ggccagcatt aagttgccat cgggcgcaaa ctcgatgatc ggaaatcgtg
     5521 ccgtgtcttt aatctcattc acagtgcggg cgtctgtaaa agcgcctctg ccgttttcta
     5581 t
//
LOCUS       DASUVS010000375         9901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1690913, whole genome shotgun sequence.
ACCESSION   DASUVS010000375 DASUVS010000000
VERSION     DASUVS010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9901
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690913"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            358..1395
                     /locus_tag="VF182_20095"
     CDS             358..1395
                     /locus_tag="VF182_20095"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6439427.1"
                     /translation="MKIYRTLLCSLFLVAFMSTNGHAQSDLFFKGKQIKIIVGFTAGG
                     IVDLWARLFTQYMGKYIAGNPDIIVQNVPGGGSMIAANQLYNIAKPDGLTLAMVSTGL
                     YFDQLMGRKEVQFDWGRFGWIGSPVRNFEVLTMRADTPYRSIEDIQKASQPPRCGTTG
                     TGATGHYFPKFVDESLGANIQIVLGYPGNRDVEVAIERGEVHCYAITKEAFLRDPGRS
                     WLKKGFVRALVQGGQKRDPLFPDTPTIYELMDKHKTPEELKRFASVLLSPGAIGRPLM
                     TPPNMPADRLRVLRDGYAKMIADPEFLADAKKREWDVEYITGEDLEATAKKAVNQSPD
                     TIARLKKILAE"
     gene            1500..2297
                     /locus_tag="VF182_20100"
     CDS             1500..2297
                     /locus_tag="VF182_20100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6439428.1"
                     /translation="MLTRVKIDNRMDGFLGKPAGAGKRPAVIHLHERYGIVQHTTDLA
                     EKFVEAGYVTIAPDLFSRFTGDRGALAAGNERCDLDDEQVLGDIDAVVAYLRTVPEAD
                     TTRIAISGVCQTGRQPILMAAKRDYICAAAVLYGAVYDADWKSDPRRPEPIDKLVEQM
                     KCPMVGIFGELDNLIPLNNIIRMIEVLAKAKKSFDVRVYADAPHGFLNDTMPGRFRPG
                     PAQVAWKQITTFMEAAFAGKFGRERALWRFESDSSVNYDFTKMKRWE"
     gene            complement(2410..2649)
                     /locus_tag="VF182_20105"
     CDS             complement(2410..2649)
                     /locus_tag="VF182_20105"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6439429.1"
                     /translation="MQLELYFYNECGFSQSVLNCMTNLGCEDKITKKNIRENPDYEKE
                     LIALCGAKTVPVLVIDGKPLRGSEEISQYLVGKFL"
     gene            complement(2656..3132)
                     /locus_tag="VF182_20110"
     CDS             complement(2656..3132)
                     /locus_tag="VF182_20110"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2995..2997),aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX6439430.1"
                     /translation="MIEVGKQAPDFELASHLGGTKFTLGQFKGQKNVMLVFYPLDWTP
                     TUTKEIPGLEALKEEFKAADTQVMGVSIDSRFSHDNWAKSCGGVSFPLLQDFHPKGEM
                     AKKYGVFLEDKGITDRATVIIDKQGVVRYAKSVTPAGERNPKELLAEAQKVNSEKR"
     gene            complement(3246..3956)
                     /locus_tag="VF182_20115"
     CDS             complement(3246..3956)
                     /locus_tag="VF182_20115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439431.1"
                     /translation="MVQPRNASTVVLLRPGDQGDFEILLTRRPPQMKFLGGYYVFPGG
                     AVHREDYSSRVLERCRGLSANDARQILGTDDNAKQALGHWIAVVRELFEEVGVLLCVT
                     ESGDKLDLRDSGAKDRIELKRQAIVRSELDFASFLESENLICDLSRAVYFDHWVTPEI
                     YSMRFDTRFYIAKLPPCQVPLSTSEEVSASLWLAPQDALSRINRHDFPILPPTTTVLA
                     ELGQIGSWRGLHDRFHLV"
     gene            complement(4154..4606)
                     /locus_tag="VF182_20120"
     CDS             complement(4154..4606)
                     /locus_tag="VF182_20120"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6439432.1"
                     /translation="MRDAATILEAGEGTLLSARGSAMFFKATRASTAGAFSLMERTLP
                     PGGRKPPAHVHTNCEEAFYVLDGEIEFSLGETITVGRRDTFVLVPGGVAHTFGNAGTT
                     PARLLVVHAPAMDAYFEELQALWSGDIPPTQDAEMELMRRHGMEPAAK"
     gene            complement(4786..5895)
                     /locus_tag="VF182_20125"
     CDS             complement(4786..5895)
                     /locus_tag="VF182_20125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6439433.1"
                     /translation="MPAKILVVDDTPKNVKLLADILTARGYNVVTAASGVEALSQVEA
                     ERPDLVLLDVVMPEMSGYEVCRKLRSNPATQLLPIVMVTALDPGEERIKGIEAGADDF
                     LTKPINQAELLARARSLIRIKELYELVQNQAAELADWNQSLERRVREQLEQLERLERL
                     KRFFSPQLAELILSGGAEDPLKTHRREITVVFLDLRGFTAFAETAEPEDVMAVLHEYH
                     AEMGKLVLQHEATLERFTGDGLMIFFNDPVPVPNPAGEAVKMAAAMRQRVAELMVKWR
                     KLDYELDFGVGIAQGYATIGAIGFEGRLDYGAIGTVTNLASRLCGEAKPGQILIPQRL
                     LGTVEDLVDAESVGELHLKGFHRPVTAYNILGLKI"
     gene            complement(5980..6369)
                     /locus_tag="VF182_20130"
     CDS             complement(5980..6369)
                     /locus_tag="VF182_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6439434.1"
                     /translation="MAGELILIIEDNEKNRKLARDVLQVKGYQTIESETAEEGLELAR
                     EKSPALILMDIQLPGIDGITALKQLRLDPKTKPIPVIAITASAMTHNRQTMLAEGFDG
                     YQTKPISLKDFLGEVEKVLATGPSKKI"
     gene            complement(6471..6761)
                     /locus_tag="VF182_20135"
     CDS             complement(6471..6761)
                     /locus_tag="VF182_20135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018624064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX6439435.1"
                     /translation="MAKQPAVYILASKRNGTLYIGVTSDLQKRVWQHKNNALEGFTKR
                     YGVHNLVYYELYGDMLTAVSREKQMKKWNRAWKLELIEKHNPNWNDLWNDII"
     gene            complement(6828..>9901)
                     /locus_tag="VF182_20140"
     CDS             complement(6828..>9901)
                     /locus_tag="VF182_20140"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6439436.1"
                     /translation="DDLAAEIQTEYPDYENRSTRTALATPLLREGIPIGLIFIRRTEV
                     RPFTDAQISLLKTFADQAVIAIENVRLFKELEVRNHQLTEALEQQTATSEVLRVIASS
                     PTELQPVLDTVIANAVTLSGAKHGHIRQIDGEILRLVAHYNESPDYIAHLQANPVRVA
                     ESLAGRAFLEGRPIQHLTTTEQPGPPSFTWSESGDRAMLAVPLLKKGAPIGNILIWCD
                     VVEPFTERQIELVKTFADQAVIAIENVRLFKELQDRNAQLSESLEQQTATSEILGVIA
                     SSPTNIQPVLDTVIANAVKLAGAKQGHVRQYDGELLNLVAYYNETPEVVAALKPSRPV
                     PESLNGRAFLERRPVQRFDAQAEYIESESGYQSPGRGAGARSALAVPLMREGAATGTI
                     LIWRDFVQPFTDRQIDLVKTFADQAVIAIENVRLFNELDTRNRQLTEALEQQTATSEI
                     LRVIASSPTDIQPVLDVVAENAARLCDATDAAIIRVNGASLKTMAHYGPISASSDPPF
                     DDRSSIPARAIIDRQTIHIHDLEAVPKDELRARFARSLGVRTVLATPLLREGVAIGAI
                     LIRRTEVRPFTDKQVALLKTFADQAVIAIENVRLFQELEARTRELVESVEELKALGEV
                     GQAVSSSLNLETVLETIVARAVDLSGTDCGVIYEYDEDAQEFNLRASHRMEQEAVEAL
                     RAARIKAGEGAAGRAASTRAPVQIADTFDQRERSTSRIRPLLNRLGYRSLLTVPLLRE
                     QKILGGLTVWRRQAGEFDPEVVNLLQTFATQSALAIHNAQLFREIQAKGRELEAANRH
                     KSEFLANVSHELRTPLNAIIGFSEVLLEKLFGALNDKQNEYVDDILSSGRHLLSLIND
                     ILDLSKIEAGHMELELTTFDLPNALDNALVLIRERASRHGVRIERVVDEQLGEFRGDE
                     RKIKQVLVNLLSNAVKFTPEGGKIQVRASLDNSAVQISVSDTGIGIAPEHHDAIFEEF
                     RQVGSDYARKREGTGLGLTLTKKFIEMHGGRIWVESEPGKGSTFTFTLPMN"
BASE COUNT         2218 a         2742 c         2579 g         2362 t
ORIGIN      
        1 cgcccggatc agcacgtcgg ctggcgttct gacacggagc cggagaatcc tgctgctgtt
       61 atcgatactg ttcgcggcgc tttcaatgag gcggggcggg ccgacgagcg ctagggatgg
      121 caatcatgtt tcgaagcaca gtcaccagcc tattctttcg cttgcacgct caccgcgcgc
      181 gctcatagtt ggatggcgta tttgagaact tcgtctgagc tcacatcgtg actaaggcca
      241 gggagcatgc cgatgtgatc acggctctcg tcgcaaccgt gactgaacac gttcacttta
      301 ttgcgagtag gcagccgcat atgtcagatc attcgctgtt tggtgaggga cccgtagatg
      361 aaaatatatc gcacgttgtt atgcagttta ttcttagtag ccttcatgtc gaccaatggc
      421 catgcccagt ccgacctgtt cttcaaaggc aagcaaatca agatcatcgt tggctttacc
      481 gccggtggca tcgtcgatct atgggcgcga ttgttcaccc aatatatggg caaatacatt
      541 gccggcaatc ccgacatcat cgtgcaaaac gtgcccggcg gcggctcgat gatcgcggcg
      601 aaccagcttt ataatatcgc caaaccggac gggctgacgc tggcgatggt ttccacggga
      661 ctctacttcg atcaattgat ggggcgtaag gaagttcaat tcgattgggg caggttcggc
      721 tggatcggtt ctccagtgcg caatttcgaa gtactgacga tgcgcgccga cacgccgtat
      781 cgaagcatcg aggatattca gaaagcatcg cagccgccgc gttgcggcac cactggcacc
      841 ggagccacgg gtcattattt tccaaagttt gtcgacgagt cgctgggcgc gaacattcaa
      901 atcgtattgg gctatccggg gaaccgggac gttgaagtgg ccattgaacg cggcgaggtt
      961 cactgctacg cgatcaccaa ggaggcattc ctccgtgatc cgggccgttc atggttgaag
     1021 aaaggatttg tccgcgcgct ggtgcaaggc ggacaaaaac gcgatcctct atttccggat
     1081 acaccgacga tttatgagct catggacaaa cacaaaactc ccgaggagct gaaacgtttt
     1141 gcatcggtgc tgctctcacc gggcgccatc ggccggccgc tgatgacgcc gccaaatatg
     1201 ccggcggata gactcagagt tctgcgcgat ggctatgcta agatgattgc cgatccggag
     1261 tttctcgccg atgccaaaaa acgcgagtgg gatgtggaat acatcactgg cgaagacctg
     1321 gaagcaacgg ccaagaaagc ggtaaatcag tcgccggaca cgatcgcacg actgaagaag
     1381 atcttggcgg aatgagccat tctgagcaag tcgatcgagc tttcattccg gcccggttag
     1441 aaaaatcaca aatgtcggtg gggcatctta gcgccgcacc atagtagggg agacaatcta
     1501 tgcttacacg cgttaaaatc gacaatcgaa tggacggctt tctcggtaag ccggccggcg
     1561 ccggcaaacg tccggcggtc attcacctgc acgagcgtta cggcatcgtg caacacacaa
     1621 cggatctggc agaaaaattc gtcgaggccg gatatgtgac gatcgcgccg gatcttttct
     1681 cccggttcac tggcgaccgc ggcgcgctgg cagcgggaaa cgagcgatgt gacctggacg
     1741 acgaacaagt tctgggagac atcgatgcgg tggtcgccta tttacgcaca gtgcctgagg
     1801 ccgacacgac gagaatcgcg atctccggcg tgtgccagac cggccggcag ccgattctaa
     1861 tggcggcaaa gcgcgactac atctgcgccg ccgcggtact ttatggggct gtttacgacg
     1921 ccgattggaa atccgatccg cgccggcctg aacccatcga caagctagtc gagcagatga
     1981 aatgtccgat ggtgggaatc ttcggtgaac tggacaatct gatcccgttg aataacatca
     2041 ttcgcatgat cgaggtgtta gccaaggcaa agaaaagttt tgacgttcgc gtgtatgccg
     2101 atgcgccgca cggctttctc aacgatacca tgcccggccg ttttcgtccg ggaccggctc
     2161 aagtcgcttg gaaacagatc actacgttta tggaagccgc ctttgctgga aagtttggca
     2221 gggaacgggc gctatggcgt ttcgaaagcg attcgtcggt gaattacgat tttaccaaga
     2281 tgaaacggtg ggagtagtct gcgcttcgcg cagaatctca gatctcagat ctaaagaaaa
     2341 aatccgtact ctcgcaaaaa agggagcgga atttcttccg ctcccttttg ttacattctt
     2401 tactcttgtt tagagaaact ttcctacaag gtactggctg atttcctccg aaccgcgcag
     2461 cggcttaccg tcgatcacga gaacgggaac cgtttttgca ccacagagcg cgattaactc
     2521 cttctcgtag tcgggattct cccgaatatt tttcttcgtg atcttatctt cgcagcccaa
     2581 gttggtcatg caattcagca cgctctggga aaagccgcac tcattgtaga aatacagttc
     2641 taactgcata cttgactaac gcttttcgct gttaactttt tgcgcttccg ccaaaagctc
     2701 cttcgggttc ctttcaccgg caggagtaac ggattttgcg tagcgaacca cgccttgctt
     2761 atcgatgatg acggtggcgc ggtcggtgat tcccttgtcc tcgagaaaaa cgccgtactt
     2821 cttcgccatc tcccccttgg gatgaaagtc ctgtaatagc gggaatgaca caccgccgca
     2881 cgactttgcc cagttgtcgt gagagaatct gctgtcgatg cttacaccca taacttgggt
     2941 gtccgcggcc ttgaactctt ctttgagagc ttccaagccc ggtatttcct tggttcaagt
     3001 gggggtccaa tccaacggat agaacaccaa catgacattt ttttgcccct tgaactggcc
     3061 gagcgtaaac ttggttccgc ccaagtgact cgccagttcg aagtcaggag cttgtttgcc
     3121 gacttcgatc atgatgattc ctccttttta gttatcgtta gcgatgctct cactatatct
     3181 aaatgccaag tcttttcaat attagagcga gcgaaagcag ctgaatcaga tagcgttgat
     3241 ctgcatcaaa cgaggtgaaa acgatcgtgc aagcctcgcc agctgccgat ctgtccaagc
     3301 tcggccagca ccgtcgtggt cggcggcaga attgggaaat catgccgatt gattcgcgac
     3361 aaagcatcct gtggcgcaag ccacaaacta gcgctgactt cttccgaagt ggatagcggg
     3421 acctggcagg gtggcagctt ggcaatgtaa aagcgggtat cgaagcgcat cgaatagatc
     3481 tcaggagtca cccaatgatc gaagtaaacc gcccgggaga gatcgcaaat gagattttcc
     3541 gactccagga atgaggcgaa atccaattcg cttctcacaa ttgcttgccg cttgagctcg
     3601 attcgatctt tggctcctga gtcgcgcaag tcgagtttat ctccagattc cgtcacacaa
     3661 agaagcacgc cgacctcttc gaagagttct cggaccaccg cgatccaatg acctagcgct
     3721 tgcttggcat tgtcatccgt accaagaatc tgtcgggcat cgttggccga cagcccgcga
     3781 catctttcta ggaccctgga tgaataatct tctcgatgaa ccgcaccccc gggaaagacg
     3841 taatagccgc cgagaaattt catctgcggc ggccgccgag tcaaaagaat ttcaaaatcg
     3901 ccttgatcgc cgggtctcag gagaacgacc gtggaggcat tgcggggttg aaccatcaat
     3961 tgatctcaaa atccgctgcg cggagcaaat cagtctttca aacgcaatca gtcatctctc
     4021 tccctcatca cgaaatcgtc ctgaaattgg atatcatcga tatccagtga agaccgattg
     4081 agggagggcc gaagaattcg ctccgcattg tcatgctgac gagtaaggtt ctaacgaggt
     4141 ccttgacgca aggtcatttc gccgcaggct ccatgccgtg ccgacgcatc aactccatct
     4201 cggcatcctg cgtgggagga atgtctccgg accagagcgc ttgcaattct tcaaagtaag
     4261 cgtccatcgc cggcgcatga acaaccaaca agcgggccgg cgtcgtgcct gcattgccaa
     4321 acgtgtgagc caccccgccg ggcacgagca cgaatgtgtc acggcgtcca acggtgatag
     4381 tttcgccgag agagaattct atttcgccgt cgaggacgta gaaagcttcc tcgcagttgg
     4441 tgtgaacgtg agccggcggt tttctgccgc cgggtggcaa agtgcgttcc atcaatgaga
     4501 aggcgccggc cgtactcgcc cgcgtcgcct tgaaaaacat ggcgctgccg cgcgctgaaa
     4561 gcagcgtccc ctcacccgcc tcgagaatag tagccgcgtc tcgcataata acttcctacg
     4621 aagtcaaaat tcgaaattcg aatatcgaaa tccgaccaaa cttcaatcaa atggacccga
     4681 aattcaaaac atcccaaact ttacgaggaa cggttttgaa cattgtttgt ttctttcgct
     4741 catttgaatt tgtttcggat ttcggatttc gatattcagg tttgtttaaa tttttaagcc
     4801 gagaatgttg taagccgtaa ccggacgatg aaagcctttg aggtggagct cgccaactga
     4861 ttcagcgtcg accagatcct ccacggttcc gagcagacgc tggggaatca agatctgccc
     4921 gggcttggct tcaccgcaca atcgcgacgc gagattcgtg acggtgccga tcgcgccgta
     4981 gtccaaacgt ccctcgaatc cgatggcgcc aatggtggcg taaccctgcg cgatgccaac
     5041 tccgaaatcc agttcgtagt ccaacttgcg ccatttcacc atcagttctg ccactcgttg
     5101 gcgcattgca gcagccatct tgactgcctc tcccgccgga ttgggcaccg gaactggatc
     5161 gttgaagaaa atcatcaagc catcgccggt aaaacgttcc agagtcgctt catgctgaag
     5221 aaccagctta cccatttcgg cgtgatactc gtgtagaact gccattacgt cctcaggctc
     5281 tgcggtctcg gcaaaagcgg taaagccgcg cagatccagg aacaccacgg tgatctcgcg
     5341 ccgatgagtt ttaagcgggt cctcggcgcc tccagacaag atcaactcgg caagctgagg
     5401 agagaagaaa cgtttcagtc gttccaaacg ttccaattgt tccagctgtt ctcggacacg
     5461 ccgttctaga ctttgattcc aatcggcaag ctcggcggct tgattttgca ctagctcgta
     5521 aagctcttta atgcggatca gcgatcttgc ccgcgcaagc agctccgctt gattgatcgg
     5581 cttcgtcaga aaatcgtccg cgcccgcctc aatccctttg atccgttctt cgccggggtc
     5641 cagagcggtg accatcacga tcgggagaag ttgcgtggcg ggattgctgc ggagcttacg
     5701 gcaaacctca tatccgctca tctcgggcat gactacgtcc agaagcacca ggtcaggccg
     5761 ctcggcttcc acttgagata aagcctcgac gccagaggca gcagtcacga cgttgtaacc
     5821 ccttgccgtc aatatgtcgg caagcagctt gacgtttttc ggcgtgtcat cgacgacgag
     5881 gattttggca ggcatgctca tgactgttgt cttatggttc gaaacctcgg gatttgcggg
     5941 cagttgtttt caccacgaag accacgaagg acacgaaact tatatctttt tactcggtcc
     6001 cgtcgcgagt accttttcta cttcgccgag gaaatctttc aagctgatcg gtttggtctg
     6061 atagccatcg aatccttccg ccaacatcgt ctgccggttg tgtgtcatcg ccgatgcggt
     6121 gattgcgatc actggaatgg gtttggtttt cggatctaaa cggagctgct tcagcgccgt
     6181 aatcccatcg atgcccggta gctggatgtc catcaaaatg agcgccggag atttttcccg
     6241 agctagctcg agcccttctt cggcggtctc ggactctatg gtctggtagc ccttgacctg
     6301 cagcacatcg cgagcgagct tccggttttt ctcgttgtcc tcaatgatca gtatcagttc
     6361 gccagccatc ttatccttca ctctgttcgt ggatatgatc cgacaagttc aatcactcat
     6421 ggttcggcaa tcgtcattcc cgcgcacgcg ggaatccagt tccccctctt tcaaatgatg
     6481 tcattccaca aatcgttcca atttggatta tgcttttcga tcaattccag cttccaagcc
     6541 cgattccact tcttcatctg cttctcccta ctcacggcag tcagcatatc gccatatagt
     6601 tcataataca ccagattgtg aaccccgtat cttttggtaa acccttcgag tgcattgttc
     6661 ttatgttgcc atactcgctt ctgaaggtcg ctcgtgacac cgatataaag cgtgccattg
     6721 cgcttactgg ctagaatata aaccgccggc tgtttagcca tccaacgtgg attcccgttt
     6781 tcacgggaat gacgatagaa tcgaactcaa ttcatacccg aaaaatatca attcatcggc
     6841 agcgtgaagg taaacgtcga gcctttcccg ggctcgcttt ctacccagat tctgccgccg
     6901 tgcatctcga taaacttctt cgtcaatgtc aggcccaagc ctgtcccttc ccgctttcgt
     6961 gcataatcgg accctacttg ccggaattcc tcgaagatcg cgtcatgatg ctccggcgcg
     7021 atgccaatgc cggtgtcgct tacggagatt tgcaccgcgc tgttatccaa gcttgcgcgt
     7081 acctggatct ttcctccctc cggtgtgaac ttcactgcat tggaaagtaa gttgaccagc
     7141 acctgtttga ttttgcgctc atcgcctcgg aactcaccga gttgttcatc aaccacgcgt
     7201 tcgattctaa cgccgtgccg ggaagcgcgc tcccttatga gaaccaacgc gttatccagc
     7261 gcgttgggca aatcgaacgt cgtcaactcc aactccatat gccccgcttc gattttggaa
     7321 agatcgagaa tgtcgttgat cagcgagagc agatgcctgc cagaggaaag aatatcgtcg
     7381 acgtactcgt tttgtttgtc attgagcgcc ccgaacagtt tttccaacag cacctccgag
     7441 aaaccgatga tcgcattcag tggcgtgcgt aactcatgcg agacattggc gagaaactcc
     7501 gatttgtggc gattggcggc ttccagttcg cggcccttgg cctgaatctc gcgaaacagt
     7561 tgcgcattgt gaatggctag agcggactgt gtcgcaaacg tttgcagcaa gttcaccact
     7621 tcgggatcga actcgcccgc ttgccgccgc cagactgtca ggcctccgag aattttctgc
     7681 tcgcgcaaca gcggcaccgt tagaagcgag cgataaccta ggcggttcag taacggtcta
     7741 atcctcgatg tcgaacgctc acgctgatca aaagtatccg caatctgcac cggggcccga
     7801 gtgctcgctg ctcgtccggc agcgccttcg cccgctttga tccgcgcggc tctaagagct
     7861 tcgactgctt cctgttccat gcggtggcta gcccgcaaat taaattcttg cgcgtcttca
     7921 tcatattcgt agatcactcc acagtcggtc cccgatagat cgacggcacg ggcgacgatc
     7981 gtttcgagca cggtctcgag attaagactc gagctcacgg cctggccgac ctcccccagc
     8041 gctttcaatt cttcgactga ttccaccagc tcgcgcgtcc gcgcctccaa ttcttggaac
     8101 agccgcacgt tctcgatggc gatcacggcc tggtcggcga aagttttcag cagcgcaacc
     8161 tgcttatcag taaacgggcg gacctcggtg cgccgaatca gaatagcgcc gatggcaaca
     8221 ccttcgcgga gcagcggtgt tgcaagcacc gtccgcaccc ccagacttct agcaaagcgc
     8281 gctctcagct cgtcttttgg aacggcctcg aggtcatgaa tgtgaatggt ttgccggtca
     8341 atgatggctc gggcaggtat ggagcttcgg tcatcaaatg gagggtcaga actggccgaa
     8401 atcggcccgt aatgagccat ggtcttcaag gaggcgccat tgacacgaat gatcgcagca
     8461 tcagtagcgt cacaaagccg tgccgcattc tcggccacga catctaagac cggctggatg
     8521 tcggtcggtg agctggcgat gacccgcaga atctcgctcg tcgccgtctg ctgctccaac
     8581 gcttccgtga gctgccggtt gcgagtatcg agctcattga agagccggac gttttcgatc
     8641 gcaatcaccg cttggtcagc aaaggtttta acaaggtcaa tttgacgatc cgtaaacggc
     8701 tgaacaaagt cgcgccatat taaaatggtc ccggttgctg ctccttccct catcagcggc
     8761 actgctaagg cgctacgcgc gccggctccg cgaccaggac tctgataacc agattcagat
     8821 tctatgtact ctgcttgagc gtcaaagcgt tgaacaggtc ttcgctccag aaacgcgcga
     8881 ccattcagac tctctggaac tggtcggcta ggtttaagag cagctacgac ttcgggagtt
     8941 tcattgtagt aagctacaag atttaacaac tcaccatcat actggcggac atggccttgc
     9001 tttgcaccag cgagtttgac ggcattcgca ataaccgtat ccaacacggg ctgaatattg
     9061 gtcggtgagc tagcgatgac gcctagaatc tcgctggttg ccgtttgctg ctctaatgac
     9121 tcgctcagtt gagcattgcg atcctgcaat tccttaaaca accggacgtt ctcgatcgcg
     9181 atgaccgcct ggtccgcgaa ggtcttaacc agctcaatct gccgttcagt aaatggctct
     9241 acaacatcac accaaatcag gatgtttccg attggcgctc cctttttcag cagtggtacg
     9301 gccaacatgg cccgatctcc tgactccgac catgtgaagc tagggggacc gggctgctcc
     9361 gtggtggtaa ggtgctggat tggtctcccc tctaaaaagg cacggcctgc cagactctca
     9421 gcaacgcgta cagggttggc ttgtaagtgt gctatgtagt ccgggctctc attataatga
     9481 gcgaccaagc ggagaatctc accatcgatt tgacggatat gaccatgctt cgcacctgaa
     9541 agtgtcacgg catttgcgat caccgtatca agtacaggct ggagctcggt cggcgagctt
     9601 gcgatgacgc gcagcacttc gctggttgcg gtttgctgct ccaaagcctc agttagctga
     9661 tggttgcgta cttcgagttc tttgaacaag cgaacgttct cgatggcgat caccgcctgg
     9721 tcggcgaagg ttttgaggag agaaatttgt gcgtcagtga acggtcgcac ctcggtacga
     9781 cgtatgaaga tgaggccgat tggaattcct tcgcgcagca acggtgtcgc taatgctgtg
     9841 cgtgtactcc tgttttcgta gtctgggtat tccgtctgga tttccgctgc caagtcatcc
     9901 a
//
LOCUS       DASUVS010000376         8340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_159786, whole genome shotgun sequence.
ACCESSION   DASUVS010000376 DASUVS010000000
VERSION     DASUVS010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8340
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159786"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(466..1743)
                     /locus_tag="VF182_20145"
     CDS             complement(466..1743)
                     /locus_tag="VF182_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEX6439437.1"
                     /translation="MSLKQPFITSLALISMLLLSCSSETRSQLSSSQYAPGTGPADKL
                     QLPAPFATASVRNTSKVIGWPLGKMPTAAPGFAVHLFAEQLNNPRQAYVLPNNDVLVV
                     EAIRVFPDRPAKSSNRITLFRDTNNDGKPEVREVFLTGLNMPHGMLLLGNWFYVGNTD
                     GVVRFPYRAGQTKIDGGGEKILDLPAGGHYTRNLVADPAGKKIYVAVGSSSNFDEDGR
                     DAKDPRRAAILEINPDGSGMRVFASGLRNPVGMDWEPKTKILWTVVNERDLLGDDLVP
                     DYLTSVKPGAFYGWPYSYFGQNEEPRKKGQRPDLVAKAVKPDYAVGAHTAALGLAFYD
                     GKSFPARYHGGAFIGMHGSWNRSKMVGFKVAFVPFDNGRPAGPMEDILTGFIADESKF
                     EAYGRPVGVTVAPDGSLLVADDAGGKVWRVSAK"
     gene            1944..3362
                     /locus_tag="VF182_20150"
     CDS             1944..3362
                     /locus_tag="VF182_20150"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6439438.1"
                     /translation="MHYRDLRGWLEGVESFGELRVVEGADWNQEIGAIAELAARSETP
                     PAVLFDKIIGYPAGRRVLTGIHNPTLKRQCLTNHLPLDYTRDQFIAAWKERLDHPTLI
                     PPRVVDSGPLMENVFEGGDIDLFSLPAPFWHEGDGGRYLGTLDITISRDLDEGWINLG
                     CYRVMVHDKDTLALYISPGHHGNIHRQKFFGRGQSFPVAISFGPDPLLWFFGQMDVPP
                     GESEYDYAGGVRGEAFEVILGKNTQLPIPAYSEVAIEGEVIPGTQIPEGPFGEFTGYY
                     AGGLRSEPMLKVKRLMYRDDPIITGAPPFRPAPGAESNLIRAAYILNYLDRAGVPDVR
                     GVAYYQTRMLVVVAIKQRYPGHAKQAALIAAQCRAAALLTRYVVVVDDDIDIWDSNEL
                     LWALCTRTDPIKDIDILRRCWSNPIDPIIPPEERGFQSRGIIDACRPYEWIHEFPKVS
                     GASPELKKKMLEKFGASLAGNK"
     gene            3391..4368
                     /locus_tag="VF182_20155"
     CDS             3391..4368
                     /locus_tag="VF182_20155"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439439.1"
                     /translation="MKPTMTVVSILVLAVNLAIPDSARAQQQTKKPLEKVRLTVAAKA
                     LTYLPYYFGKSKGLFEKEGLDLETIIMRPPIGVTALAAGEVDYTGSTGVSLRAAMKGL
                     PVKALIFIQTRLSFSLIGQPGMTPAKIKNVAVSGIGSLAHYGAQTVLKRLGRDGPGDK
                     INYIATNTTANSYAALTGKAADAVILTAPYTSMATLAGYVDLGDTFDVRDVQGGLVGR
                     MSHIQNHREQIKAVLRAVLRSNDMILKNDGEVIPFIQKEFGLEQNVAADSYRQIKQVL
                     NADGDIEEPVLKSILDKIRQESGIAAEIPVDRLVDLSMLREARAELRKR"
     gene            4525..4986
                     /locus_tag="VF182_20160"
     CDS             4525..4986
                     /locus_tag="VF182_20160"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6439440.1"
                     /translation="MKSLLAAITLIMLVLTQTRAFADLPEPKAGPQNPNMEAGRKAID
                     AKDYKAAVGHLTKAVQELPNDADAHNMLGYSYRKTGNFDKSMEHYQRALKIDANHRSA
                     HEYLGELYLDMNQMANAEKQLVALKKACPLLGKCEEYDDLKQAIENHKAKK"
     gene            5076..5798
                     /locus_tag="VF182_20165"
     CDS             5076..5798
                     /locus_tag="VF182_20165"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439441.1"
                     /translation="MAEIVKATIGFVSGGTGSMPHYSSFLPMIPESVKFDFVGLQLYN
                     ESLYQIADKKQIIVERLQELIAQRHWNGVILSAAPTQVLNPGLFDALKAALAVPFTTA
                     LHACVTALRVYGAKRVLLLTPFDQRLNQLICGHLEQLRVDAVAPHPFENLGVPRRMDP
                     DEVFELTKKNLSATDPVDAIYFQGAVLDPIKVLEKIERELGMMVIASNPAMLWHVLSK
                     LGLSYPISGYGRLMREWPAIIS"
     gene            complement(6012..6362)
                     /locus_tag="VF182_20170"
     CDS             complement(6012..6362)
                     /locus_tag="VF182_20170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439442.1"
                     /translation="MKRSTALWVIILAFFLTAACDQIFDRDSGIFNWPGSARDLEGGW
                     YVNGDPKLRAEIVSTPSGLEARNERGQSTRLDLTSGGSVRALDWEGGLRGTVRRDQID
                     WQNGTSWTRQARNR"
     gene            6675..7679
                     /locus_tag="VF182_20175"
     CDS             6675..7679
                     /locus_tag="VF182_20175"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439443.1"
                     /translation="MTIYRLVIGCMVLIGSYGSSDLLAQEKKLEPLRSGGGSASAAQM
                     SIWFAKEANYYEKNGLNVELISIPGSSLALQAMLSGELPIIQAGGAGPIQVALSGTDT
                     VIIATIAKKFNWWIFSQPNIGRMEDLRGKVFGTTRFGTQSDLASRIALRLHGLDPERD
                     VTMVQTGGPAETVTAMMTGKVHAAAISPPATLQAKRVKLKDLMDLSKLDVEYHVNGLV
                     TTRRYLKTNEDVVRRFLRAYIEGAVRGQKDKAFAMKTMGKYFRSDDREVLEETYEMSI
                     KNGFSVPPYPAGIASLLQELEKQFPKARGAKPEDFADGRLVRELDQSGFIKSVLVSGK
                     "
     gene            complement(7888..>8340)
                     /locus_tag="VF182_20180"
     CDS             complement(7888..>8340)
                     /locus_tag="VF182_20180"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX6439444.1"
                     /translation="VDQAVRSMTTLNATASNVMRKFAVHACSDITGFGILGHGLGMAC
                     GSGVTLVIESAKLPLLQDATQLAEKGYITGGCKRNREFLQDKISIEKIREGLIEVALD
                     PQTSGGLLIAVAKRHASQLLEELKSSGIDAATQVGYATSQQKAWVRLV"
BASE COUNT         1955 a         2207 c         2327 g         1851 t
ORIGIN      
        1 ggttgaagat tatttttgtg atgtggagag atcataaacc ttatgacgaa acccatcact
       61 tggtgaatat tgcacgacag aaattgcgcc aggcggcgtg aaaaaaaccg atcgaggctt
      121 gacagggctc atagaaagtc ttcgctcgcc gatgtcttgc gcgcctcggc tctaactctt
      181 tttgagcagc ctgcaatcgg cgttgtccat ccacctgcta acccaatcac gagctgtcgc
      241 tttcgattgc gtgaatagcg cagtacccga ctactttcct taactcttct acgggcccgc
      301 tagtatagac tcgtcccaac gtctacagcc gtcggtcatt ttcggtgcag tctgttccag
      361 tcgggagctt tttcatagtt ccatactgga gcagtcatag cagcgttcca gatgctttga
      421 caaactaacg ctgttgcgga tcattttccg ttttgcagtt ccggttcact tcgcgctcac
      481 ccgccagact tttccgccgg catcgtcggc aaccagcagt gagccgtcgg gcgccaccgt
      541 aacgccgacc gggcggccgt atgcttcgaa cttgctttca tccgcaataa atccggtgag
      601 aatatcttcc atcgggccgg cgggtctgcc gttgtcgaaa gggacaaagg ctactttaaa
      661 gccgaccatc ttcgaacgat tccatgagcc atgcatgccg atgaaggcgc cgccgtgata
      721 gcgcgctggg aaagatttgc cgtcataaaa agccaatccg agcgcggcgg tgtgcgcgcc
      781 cacggcgtag tccggtttca ccgctttggc aaccagatcg ggccgttggc ctttcttgcg
      841 gggttcttcg ttttgaccaa agtaggagta gggccagccg tagaatgcgc cgggcttgac
      901 gctggtgaga tagtcgggaa ctaaatcgtc ccctaggaga tcgcgctcgt taaccacggt
      961 ccacagtatt ttggttttcg gctcccaatc cattcccacg ggattgcgca ggccgctggc
     1021 aaaaacgcgc atgccgcttc catcgggatt gatctccaag atcgccgctc gccgtggatc
     1081 cttggcatct ctgccatctt catcgaagtt agagctggag cctaccgcga catagatctt
     1141 tttaccggcg ggatcggcga ccaagttgcg ggtgtagtgt ccacccgcgg gcagatcgag
     1201 gattttctcg cctccaccgt caatcttggt ttggccggca cggtacggaa agcgcactac
     1261 tccatcggtg ttaccgacgt aaaaccagtt tcctagcagc agcatgccgt gcggcatgtt
     1321 caagccggtc aagaaaactt cacgaacttc cggtttgccg tcattattag tgtcgcggaa
     1381 caaggtgatt cggtttgacg acttagccgg acgatcgggg aatacgcgga tcgcctcgac
     1441 gaccagaacg tcattgttcg gcagcacata ggcctgccgc gggttattga gctgctcggc
     1501 aaataggtgg acagcaaaac caggcgcggc ggtcggcatc ttgccgagcg gccagccgat
     1561 cactttcgaa gtgttgcgta cggacgctgt tgcgaacggc gcgggcaatt gcagcttatc
     1621 tgccggtccc gtgccgggcg cgtattgact gctggataac tgagaacgag tttcgctgga
     1681 acatgaaagg agcaacatcg agatcaacgc gaggcttgta ataaatggtt gtttaagcga
     1741 catcggattc tcctccagtg taggacagaa aaattgcctg caatcggtac tgaattagaa
     1801 catagacaat ccgtgatttg ctgtgaaggg gcaatcatct tcccttagtc cggcgttgga
     1861 gtgcatccgt agatagaaac tcgcttctcg gttgtagcgg cgtcttgtcc gttctaagat
     1921 cgccaactca caggggggac tcaatgcatt atcgcgattt gcgcggctgg ttagagggtg
     1981 tggaatcttt cggcgagctc agagtcgtgg aaggagccga ttggaatcag gaaatcggcg
     2041 caatcgccga actggcggct cgttccgaga cgccgccagc ggttctcttc gataaaatta
     2101 tcggctatcc ggcgggccgg cgcgtgctga cggggattca caatccgaca ttgaagcgcc
     2161 agtgcctgac caatcacctg ccgctcgatt acacccgcga tcaatttatt gctgcctgga
     2221 aagaacggct cgaccacccg actttgattc cgccgcgggt ggttgattcg ggaccgctga
     2281 tggaaaatgt tttcgaaggc ggcgatatag atctgttctc attgccggcg cctttctggc
     2341 atgaaggcga cggcggccgg tatcttggaa ctttggacat cactatcagc cgtgatctcg
     2401 acgaaggctg gatcaatctg ggttgctacc gtgtcatggt gcacgacaag gatacgctgg
     2461 cgctttacat atcgccgggg caccacggca acattcatcg gcaaaaattc ttcggccgcg
     2521 gccagtcatt tccggtggcg attagtttcg gaccggatcc gttgctctgg ttcttcggtc
     2581 aaatggacgt tccgccggga gaatcggaat acgattatgc cggcggtgtt cgtggcgagg
     2641 cattcgaagt gattctcggc aagaacacac agctgccgat cccggcgtat tcggaagttg
     2701 ccattgaagg cgaagtcatc ccgggcaccc agatacccga gggaccgttt ggcgaattca
     2761 ctggctacta tgccggcggc ctccgttccg agccgatgct caaggtgaag cgattgatgt
     2821 atcgcgacga tccgattatt accggagcgc cgccgtttcg tcctgcgccc ggagctgaga
     2881 gcaatctgat ccgcgcggcc tatatcttga actacctgga ccgagcggga gtgccggatg
     2941 ttcgcggagt ggcctactat caaacccgca tgctggttgt ggttgccatc aagcagcgct
     3001 atccgggaca tgccaaacaa gcggcgctga tcgcggcgca atgccgggcg gcggcgctgc
     3061 tcacccgtta tgttgtcgtg gtcgatgatg acatcgatat ttgggacagc aacgaactgc
     3121 tttgggcgct atgcaccaga accgatccga tcaaagatat cgatatcctg cgccgttgct
     3181 ggagcaatcc gatcgatccg atcattccgc cggaggagcg cggctttcaa tcccgcggca
     3241 ttatcgatgc ctgtcgtccg tatgagtgga tccatgagtt cccgaaagtg tcgggcgcca
     3301 gtccggaact gaagaagaag atgctcgaga agttcggcgc cagtttggcg ggtaacaaat
     3361 gaaaaacttt ttattctcgg agaagtttcc atgaaaccaa ccatgaccgt ggtgagcatc
     3421 ctagtgcttg ccgttaatct tgcaattccg gattcagcgc gcgcccagca acaaaccaag
     3481 aagcctttgg aaaaagtccg gctcacagtg gccgccaagg ccttgaccta ccttccctat
     3541 tatttcggca agtcaaaagg gctctttgaa aaagaagggc tcgatctgga aaccattatc
     3601 atgaggccgc ccatcggcgt gactgcctta gctgcgggtg aagttgatta caccggctca
     3661 accggagtgt cactacgcgc ggcgatgaaa ggattgccgg tgaaggcatt gatattcatc
     3721 caaacccgat tgagcttcag tctgatcggc cagccgggaa tgacgccggc aaaaatcaaa
     3781 aacgttgcgg tcagcggcat cggctcgctg gcgcactatg gcgcgcagac cgtgttgaaa
     3841 cgacttggcc gcgatgggcc tggtgacaag atcaattaca tagccacgaa tacaacggca
     3901 aatagctatg cggctctgac cggcaaagcg gcggatgcgg tgattctcac ggcgccctat
     3961 acctcgatgg cgaccttggc cggctacgtc gatctcggcg ataccttcga tgtgcgcgat
     4021 gtgcaaggag gcctggtggg aagaatgagc cacatccaga atcatcgcga gcagatcaag
     4081 gctgtattgc gcgccgtatt gcgtagcaac gacatgattc tcaagaacga tggcgaggtg
     4141 attccattta tacagaagga attcggtttg gagcagaacg tcgcggctga cagttaccgg
     4201 cagatcaagc aagtgttgaa tgccgacggt gatatcgaag agccggtttt gaagtcgatc
     4261 ctcgataaaa tcagacagga atccggcatt gccgccgaaa ttcccgtgga ccgtctcgtc
     4321 gatctttcga tgctccgcga ggcaagggcg gaactccgca agaggtagtg cagacaatag
     4381 agacagggag cgggagacag aaagcaatca gctgaagacg ccgaaattct gatgccaaaa
     4441 tccaaaatcg aaaaaccaaa atgagaggtc ttgcctttca gctatcgcca ttgataagtt
     4501 cgccatgcat caaaggaggt ctgtatgaaa tcgctattag ccgccattac gttgattatg
     4561 ctggtcctta cgcagacgcg ggctttcgcc gacttgccgg agcccaaggc cggcccacag
     4621 aatccgaata tggaagccgg acggaaggcc atcgatgcga aggattacaa ggcagcggta
     4681 gggcatctga ccaaggcggt gcaggagctg cccaatgatg ccgacgccca taacatgctc
     4741 ggctatagct atagaaaaac gggtaacttc gataaatcga tggagcacta tcagcgcgcg
     4801 ctgaagatcg atgccaacca tcgcagcgcc catgaatacc tgggcgagct ttaccttgat
     4861 atgaatcaaa tggccaatgc cgaaaaacaa cttgttgcgc tgaaaaaggc ctgcccgttg
     4921 ctcggcaaat gcgaagagta cgatgacctt aaacaggcta tcgagaacca caaggcgaag
     4981 aaatagcaaa aagagacggg gcagactagg aggcaagggc tacattgatc aacgttccaa
     5041 aaggtgaacc ttcacacgat taaggaagtt ggcaaatggc tgaaatagtc aaagctacaa
     5101 tcggctttgt cagcggcggg actggctcca tgccgcacta cagtagcttt ctcccgatga
     5161 ttcccgaatc agtgaaattc gactttgtcg gtttgcagct ctacaacgaa tcgctgtatc
     5221 agatcgccga taaaaaacag atcattgtgg agcggcttca agaactgatc gcccagcgac
     5281 actggaacgg ggttattctc agcgccgcgc ctacgcaagt gctcaatccg ggactcttcg
     5341 atgcgctcaa agcggcgctt gcggttcctt tcacgacggc tttgcatgct tgtgttacag
     5401 cgcttagggt ttacggtgcc aaacgcgtcc ttttgctgac tccctttgat cagcgtttga
     5461 atcagctgat atgcgggcat ttggagcagc tacgagtgga tgcggtggcg ccgcatcctt
     5521 ttgagaatct cggcgtaccc agacgcatgg atcccgacga agtcttcgaa ctgaccaaga
     5581 aaaatctcag tgcgaccgat cccgtcgacg caatctattt tcaaggcgcg gtgctcgatc
     5641 cgatcaaagt tctggagaaa atcgagcgcg agcttggcat gatggtgatt gccagcaatc
     5701 cggccatgtt atggcacgtg ctttcgaagc tcgggctttc ctatccgatc agcggctatg
     5761 gccggctcat gcgcgagtgg cccgcgatca tctcctagca tgtgctgaaa attctgatta
     5821 cggctgctca cgacctcata agcatccctc gtgctcgtgg ccgtgaaaac gtgatcgaga
     5881 ttcccaacga agacgaacat ggatttgacg cgaggcgtgc gaattacaaa gatgaaagcg
     5941 gaaaatctga aagcgaactc cgtttccagc tttcataatt ctatcgtagc gacaagctgg
     6001 catgaccgaa cctatctgtt gcgcgcctgc ctggtccagc tggtgccatt ctgccaatcg
     6061 atctgatcgc gccggacagt gcctcgcagt ccaccttccc agtccagagc acggacactg
     6121 ccaccgctgg tgagatccag ccgggtgctc tggccgcgct cattgcgtgc ttcgagcccg
     6181 gacggggttg aaacgatttc ggcgcgtagt ttcgggtcac cgttaacgta ccagccaccc
     6241 tccagatccc gagccgatcc cggccagtta aaaattccac tgtcccgatc aaaaatttga
     6301 tcacaagcag cggtgagaaa gaatgcaagg ataatcaccc aaagcgcggt cgatctcttc
     6361 acggtacctc ctcgttgtct ctgctaagtc tcgattccct acagaagcta ttatatcgga
     6421 ttcacgcaag ggaaaaagca tatgccgaaa gattcctcta ttttgatatc atcgaggcgc
     6481 atggcgccgc tctaacctga agacctgctg gactacccgg cgcgctgccg atatcgaaca
     6541 cgttcatcac atctgcgtgc gccctgaaga aaatagccac cgagaaagcg actcgatgcc
     6601 gccgctttct cgacaaaaaa cgctaaagct gtgttaaata ggatcagtat tcatggttac
     6661 gagagggaga tagtatgacg atttatcgat tggtaatcgg ctgcatggtc ttgattggca
     6721 gctatgggtc ttcggattta cttgcccagg aaaaaaagct cgagcccttg cgaagtggtg
     6781 gcggctcggc atcggcggcg cagatgtcta tctggtttgc caaagaagca aactattacg
     6841 aaaaaaacgg gctcaatgtc gaactcatca gtattcccgg aagctcgctg gcgctgcagg
     6901 cgatgttatc gggagagctg ccgatcatcc aggctggcgg tgcgggtcca atccaggtcg
     6961 ctctctcggg cacggatacg gtgattatcg cgactatcgc caagaaattt aactggtgga
     7021 tcttcagcca gcccaacatc ggccgtatgg aagatttaag agggaaagta tttggcacga
     7081 cccgtttcgg tactcagtcg gatctagcct cacggatcgc acttcgcctc catgggttag
     7141 atccggaacg cgacgtcacc atggtgcaaa cgggaggtcc cgcggaaacg gtgactgcca
     7201 tgatgaccgg caaagttcat gcggctgcca tctccccgcc ggcgaccttg caagcgaaaa
     7261 gggtcaaact caaggatttg atggacctgt caaaactcga tgtcgagtat cacgtcaacg
     7321 ggttggttac tacccgcaga tatctcaaaa ccaacgagga tgtcgtgcgg cgttttctaa
     7381 gagcttatat cgaaggcgcg gttcgcgggc agaaggacaa ggcttttgct atgaaaacca
     7441 tgggaaaata ttttcgcagc gatgaccgtg aagtgctcga agagacctat gagatgagca
     7501 ttaaaaatgg tttcagtgtc cctccctatc cggccgggat cgccagcttg ttacaagagc
     7561 tcgagaaaca gtttcccaaa gccaggggag cgaaaccgga ggattttgcc gatggccggt
     7621 tagtgcgcga gttggatcag agcggcttta ttaagtcggt gctggtgagc ggcaaatgac
     7681 atctgatttt ggtgctcagc gaaccaggga attttgggga agtgtagaag tccagcagtt
     7741 gctgactcgg tggtgggctg ctcaaaaaga tccgtaaccg aggcgcgcga agatcgataa
     7801 ccggaggcgt gctcctgcac gttgacgcgc aatcggtcga gagcaacgaa gcctatgggc
     7861 cttttcagca gtccgctagt gggtggttca gacgagccgc acccaggcct tttgctgtga
     7921 cgtggcataa ccgacctggg ttgccgcatc gatgccggaa cttttaagct cttcaagaag
     7981 ctgcgatgcg tgccgcttgg ccacggctat cagtagtccg cccgaagtct gcgggtctaa
     8041 cgctacttcg atgagtccct cgcggatctt ttcgatggaa attttatcct gcaaaaattc
     8101 ccggttacgc ttgcagccgc cggtgatgta gcccttttcg gctaactggg tggcatcttg
     8161 caacagcggc agcttggccg attcaatcac caaagtcaca ccgctgccgc aggccatccc
     8221 caaaccatgg ccaagaatac caaagccggt gatatccgag caggcgtgca ccgcaaattt
     8281 gcgcatcacg ttagacgccg tggcgttgag cgtggtcatc gaccgaactg cttgatcgac
//
LOCUS       DASUVS010000377         5530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1909950, whole genome shotgun sequence.
ACCESSION   DASUVS010000377 DASUVS010000000
VERSION     DASUVS010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909950"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VF182_20185"
     CDS             complement(<1..370)
                     /locus_tag="VF182_20185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439445.1"
                     /translation="MADAVDFWSQQLTEIGSGFRLGPVKFVSEILAATELAALSQAML
                     DGQRALVGPPSGFMQMKEDLIIALSDGAFVSFAMSFQNTDKRLIAIPSFHAVPFRFTN
                     VGRNLMAHELGHAIGLGHNDD"
     gene            complement(914..2392)
                     /locus_tag="VF182_20190"
     CDS             complement(914..2392)
                     /locus_tag="VF182_20190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439446.1"
                     /translation="MPPDADLIKLVRRFEPILYFHPRERFFPSDAKRYIEHSALWKST
                     LANDGKLVPADPVIKKKKIAAFENEKEADQRFLGETLSGTTFFLAPDCFFELTGWKTI
                     ERRYSNLEELANLYDPDANGEETLVKSRFWYHAEFFGAVRLRDLMNMLVSEGLDLSKV
                     FNKLIEKHPNPALLCYYFFFPGHIESLAAPCEETETGKQFASFAGEWACLALLLNRQS
                     DNQAYEPQWIGYTGRRNLDSTQGLDNERRVGMTVEEWKERIGKLNKPLPATIDDHPAL
                     FVSLGTHSLYLEPGEHKVDPYPSESFPQWCGQFDGPGALNQYLTSQPPPAEEGSPVAA
                     WGKILGAGFLLGIGPPGLIAGAVLTALEGLPLFPGSETPEDEVLAPLEAPQPDIGPEP
                     GSNNKVLVPKNVAIDDLNNEPIEWIADEDVPIDGRHYNFIVDRQAQVWWPSDDTTSGY
                     RGRWGPPVALDPFNRRAGMRFPEFWRMFFVALAKVAPQEFQD"
     gene            complement(2382..3503)
                     /locus_tag="VF182_20195"
     CDS             complement(2382..3503)
                     /locus_tag="VF182_20195"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX6439447.1"
                     /translation="MSATFELICHHVYAGWDGLPVDLSDHDNHGQAIDTQFLKDGVAP
                     DSGALRFWKSGSRVSVPASNSWRSLGGIKVEVIARRRITPILLDDHQTLVAGHNSFSF
                     FISNHSLFASFRGTSTYPLQNSDGLNSIGSPGFQEYLVPYGKWVTLGFTHNGFDTMEL
                     YAEGQLIARRTNLLGTVPSVGPLGVCIGNEPEGNDTHWDGDIDEVKVWRVNPRVMDEE
                     FFSRPVTDDVARCWARFFRSLAMALEQNPECARQIQGAIRSAVDRVRHTIIGKGPETR
                     ARYFRTCYEYLQLWRAGKLDSPEMAKLCADWCAWLRLVGVSLHDDPTLKALLESDCLK
                     KIVADCEPLNCDPDFYALVRLVVENCGQWFAPERKSDAP"
     gene            complement(3635..4519)
                     /locus_tag="VF182_20200"
     CDS             complement(3635..4519)
                     /locus_tag="VF182_20200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439448.1"
                     /translation="MGSLGADEAGEDMTVGRTNKSEERTLLVATRAPNGYADDFVLSV
                     SIENDAVLTSTPGRGVDAIHAKGTLPLPTGSTTMGTIPPGNGIIAQGNNGVVGYIHAT
                     TRNRTLENSVVAGVVGIAGGASAIGGVGPSPGVFGIGRNGVVGIEQATTRDLAFEAAQ
                     KAGVLGRGETGVSGDGANGPGVFGRGLPGVQGQSSGGPGVLAEGQTGVHATGNKGAGV
                     HATSTEEQAAILQSTRMAQLWLIPLRIKDPTALAKSTAGELLSTVWPDERGTEITSLW
                     FCTRGGTPTEASWKKLAP"
     gene            complement(4719..5105)
                     /locus_tag="VF182_20205"
     CDS             complement(4719..5105)
                     /locus_tag="VF182_20205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015178471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439449.1"
                     /translation="MNLGFAIADFDWRHHGEENLFKQRTKQVASAFIEIAGTLPKNWS
                     ADVIGTQLIRSGTSIGDNYPGDLPVEQPTKFELIINLKIAQQIGLTIPSNVLARADRV
                     IQGYGNDSNVRHQRAPSRQPLAFRSS"
     gene            complement(5185..>5530)
                     /locus_tag="VF182_20210"
     CDS             complement(5185..>5530)
                     /locus_tag="VF182_20210"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX6439450.1"
                     /translation="NKQRPDGLYVPGGPGMNDNRKRITDFALKHRMPSIYSRSEFVDF
                     GGLMSYGADLAYSYRRVAYYVDRILKGVRPGELPVEQPSKFELVINAKTAKQIGLIIP
                     PNLLARADRVIR"
BASE COUNT         1235 a         1550 c         1455 g         1290 t
ORIGIN      
        1 ggtcgtcgtt gtgcccgagt cctatcgcat ggcccagctc gtgggccatc aggttacgac
       61 cgacattggt gaaccgaaac ggcaccgcat gaaagctagg gatagcgatg agacgtttgt
      121 cggtgttttg gaaagacatt gcgaaggaaa caaaagcccc gtcagacaac gctatgatca
      181 aatcttcctt catctgcata aaacctgatg gcggccccac aagtgccctt tgcccatcga
      241 gcatagcctg actcaacgcc gcaagttcgg tggctgccag gatctcactt acaaacttca
      301 ccggtcccaa tctaaatcca ctgccaatct cggtgagctg ttgactccag aaatcgactg
      361 cgtcagccac caactgattt ctcgagtctc cctcctgccc taagacaaca atggatggta
      421 ttttgtccca accgcgagcg cggtaaggga acaatgtcgc ggggccggca gcctggctac
      481 aaccggggag caagagcaaa aatataagga gaccccgcag ttgctttctg cttatctcca
      541 cagtgtttgc tgtcatttga tcaccggatc tgcccgcgcg cacattcggc ggatcgccgc
      601 gcgattttgg atttggcgat tttggattgg atttcttgtc atcggattac gcgatccgct
      661 cgggccaaaa tgtttggcgg aatgatcagg ccgatctgct tgcggttttc gcgttgatca
      721 ccagctcgaa cttcgtcggc tgctccgccc gcatgtcgcc aggttggcac tcttaacgat
      781 tctctcgatt aatgtggcta agcgccggta actgtctgcg aggtcggcta gaacatgaac
      841 ccaccggcat ctacaagatg gctcctcatg tctctcgact aggaccggcg cttagttcag
      901 cagggagcgt aagttaatct tgaaattctt gaggcgcgac cttcgccagg gcaacgaaaa
      961 acattctcca gaactccgga aacctcattc cggcacgccg attaaaggga tcgagcgcaa
     1021 ccggcggacc ccaccgacca cggtaaccgg aagtagtgtc atcgctcggc caccagactt
     1081 gcgcttgccg gtcaacgata aagttataat gacggccgtc gattggcacg tcttcatccg
     1141 cgatccattc tattggctca ttattaagat catcaatagc gacatttttg ggaacgagga
     1201 ctttgttgtt gctgcccggc tcgggcccaa tatccggctg tggtgcttct aaaggcgcaa
     1261 gaacttcatc ctcgggggtc tccgatccag gaaaaagagg caggccttcc aaagccgtca
     1321 acaccgcgcc ggcgataaga ccaggcggcc caatcccgag gagaaatccc gctccgagaa
     1381 ttttgcccca agcggcaaca gggcttccct cctcggcagg aggtggttgg gatgtaagat
     1441 attggttgag cgcaccaggt ccatcgaact gtccgcacca ctggggaaag gactcagaag
     1501 ggtagggatc cactttatgc tcgcccggtt cgagatagag gctgtgcgtc cccaaagaca
     1561 caaagagcgc gggatgatcg tcgattgtcg ccgggagcgg cttgtttagt ttgcctatgc
     1621 gttctttcca ttcttcaact gtcatcccga cacgtctctc gttatcaagg ccctgcgttg
     1681 agtctagatt gcgccgaccg gtataaccaa tccactgcgg ctcgtaagct tgattgtcac
     1741 tttgtcggtt cagcagaagc gccaagcacg cccactcacc ggcaaaactg gcaaactgct
     1801 tgcccgtttc cgtctcctca cagggtgccg ctaaggattc gatatgaccc ggaaagaaga
     1861 aataatagca gaggagagcc ggattcggat gtttctctat gagtttgttg aataccttgc
     1921 tcaggtccaa accttcactg accagcatgt tcataagatc tcgcaggcga acagcgccaa
     1981 agaactctgc gtgataccaa aaccggcttt taacaagcgt ctcctcgccg ttagcgtcag
     2041 gatcgtagag gtttgctagc tcctctaaat tggaatatcg gcgctcgatt gttttccagc
     2101 cggtgagttc aaaaaaacag tctggcgcga gaaaaaaggt ggtgccagat agcgtctcac
     2161 caagaaagcg ttgatcggct tccttttcat tctcgaaagc cgcaatcttt tttttcttga
     2221 tcacggggtc ggcaggaaca agcttgccat catttgcaag tgtggatttc cagagcgcgc
     2281 tgtgctcaat gtaccgtttg gcatcggatg gaaagaagcg ttccctcgga tgaaagtaga
     2341 gaattggctc gaaccggcga accaatttga tcaaatctgc atcagggggc atcgcttttt
     2401 ctttccggcg cgaaccattg cccgcagttt tcgaccacaa gccggacgag cgcgtaaaag
     2461 tcgggatcgc agttgagtgg ctcgcagtcc gcaactattt ttttgaggca atccgactcc
     2521 agcaaagcct tgagtgtcgg atcgtcgtgc aaggagaccc cgaccaaccg taaccaggca
     2581 caccagtcag cgcagagctt tgccatctcg ggactatcga gttttcccgc cctccagagc
     2641 tgcaaatatt cgtagcaagt tctaaagtag cgcgcgcgag tttccgggcc ttttccgatg
     2701 attgtgtgcc gaacccggtc aaccgcgctc ctgatcgcgc cttggatctg tcgtgcacac
     2761 tcggggttct gctctaaggc cattgccagg ctgcggaaga atcgcgccca gcacctggca
     2821 acgtcatcgg ttaccggccg ggaaaagaat tcttcatcca taacgcgcgg attaacccgc
     2881 cagactttta cctcatcaat gtctccgtcc cagtgagtat cattcccctc cggctcgttg
     2941 ccgatgcaga cgcctagcgg ccccactgac ggcaccgttc ccagcaggtt agtgcgccgc
     3001 gctatgagct gcccctcagc ataaagctcc atcgtgtcga agccgttatg cgtaaagcct
     3061 aaggtgaccc atttgccgta gggaactaga tactcctgaa agccgggcga accgatggag
     3121 ttcagtccat cgctgttctg taacgggtaa gtcgacgtgc cgcgaaacga agcgaacaaa
     3181 gaatggttac tgataaaaaa gctgaaagaa ttgtgaccgg cgacgagagt ttgatggtca
     3241 tctagcagga taggagtgat acggcggcgc gcaatcactt ctactttaat cccgccaaga
     3301 gagcgccagc tattgctcgc aggtacggat acccggctgc cgctcttcca gaaacgaagc
     3361 gcgcctgaat cgggcgcgac gccgtctttc aaaaactgcg tatcaattgc ctgtccatgg
     3421 ttatcgtgat cggacaaatc cactggcagt ccgtcccatc ctgcatagac gtgatggcaa
     3481 atcagttcga aagttgcact catagcaacc accgattacg aatgattttg ctggttaaga
     3541 aaagcgtgtc gccaactcac aggcaagctg cctgccgctg tttgacgcga ccaagagccg
     3601 cgggagcttt agccgatagc gatcatcggc gttgttaagg agccaatttt ttccagctcg
     3661 cctccgtcgg ggtgccgcct ctggtacaga accagagact ggtgatctcc gtgcctcttt
     3721 catcgggcca gacagtggac aatagttccc cagccgtcga tttcgctagt gccgtcggat
     3781 ccttgatacg cagcggaatg agccagagct gcgccatcct cgtcgattgc agaatggccg
     3841 cctgctcctc tgtcgaggtt gcgtgcaccc ccgcgccttt gtttcccgtt gcatggacac
     3901 ccgtttggcc ttcggccagc acgccgggac cgccgctcga ttgtccttgc acaccgggca
     3961 agccgcggcc gaacacgccg ggaccattcg cgccatcgcc actgacgccg gtctcgccgc
     4021 gtcccagtac gccggctttt tgcgctgctt cgaaagcgag atcgcgtgtc gttgcctgtt
     4081 cgattccgac gactccgttc cgaccgatgc cgaatacgcc gggagacggt ccaacgccgc
     4141 cgatggcaga cgctccaccg gcaataccga ccacgcctgc cacgacggag ttctccagag
     4201 tccgattgcg cgtagtcgca tgaatgtagc cgacgacgcc attgttaccc tgggcgatga
     4261 tgccattgcc gggcggaatc gttcccattg tcgtactacc ggtaggtaga ggaagcgtgc
     4321 ccttggcatg gatggcatcg acaccgcgcc cgggggtgga tgtgagcacc gcatcatttt
     4381 cgatcgagac actcagaaca aaatcgtccg cgtagccgtt cggagcgcgg gtagcgacca
     4441 ggagagtgcg ttcttcgctc ttgttcgtcc gaccgacagt catgtcttcg ccagcttcat
     4501 cagctccaag acttcccatg agacctcctc ggatcactcc actgagccgt tgttgcagac
     4561 actaaaaacg aaatctgttg gtcaaagaaa gcggggtagt aacgcttcgg cttttataat
     4621 cgagatattg agttgtcaac ttttttctat ccaacactgg cacacatgag aagtaaattt
     4681 ctagatgaaa actaattcca caggatcgcg atcctctatt atgacgatcg gaatgctaat
     4741 ggttgcctgc ttggcgcgcg ttgatggcga acgttggaat cgttgccgta gccttggata
     4801 acccgatctg ctctagccaa gacattggat ggaatggtta atccgatctg ctgtgcgatc
     4861 ttcagattga tgatcaattc aaatttcgtg ggctgctcaa ccggaaggtc gccaggatag
     4921 ttatcaccaa tcgaagtgcc cgatcggatc aattgcgtac cgattacatc tgcactccag
     4981 ttcttcggca gcgtgcccgc aatttcaata aaagctgatg ctacttgctt cgtacgttgt
     5041 ttgaataagt tttcttcgcc atggtgtctc caatcaaaat cggcaatcgc aaatcctaaa
     5101 ttcatttgat cacccggttt gcgcgcgcca gcacgtgggg tgcggaacgc ctgtgatttt
     5161 agattttcga ttttggattg gagatcatcg gatgactcga tcggctctag ccaacaggtt
     5221 cggcggaatg atcaggccga tctgcttagc ggttttcgcg ttgatcacca gttcgaattt
     5281 actcggctgc tcgacgggta attcccccgg tctgactccc ttgaggattc tatccacgta
     5341 gtaagcgacg cgccgatagc tgtacgcgag atcggctccg taggacatga gcccgccgaa
     5401 atcgacgaat tcgcttcggc tgtaaatgga cggcattcgg tgcttcaacg cgaaatcggt
     5461 gatccgtttt ctgttgtcat tcattcccgg tccaccgggc acgtagagtc catcgggacg
     5521 ctgcttgttc
//
LOCUS       DASUVS010000378         3791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1693738, whole genome shotgun sequence.
ACCESSION   DASUVS010000378 DASUVS010000000
VERSION     DASUVS010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693738"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1554)
                     /locus_tag="VF182_20215"
     CDS             complement(<1..1554)
                     /locus_tag="VF182_20215"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX6439451.1"
                     /translation="MTAEFTTEEQKRLIGFFTNLERPIFGLKLPQEVAGALFSRYSRS
                     SKSLRRTFLDEFLGDPELGLQDLLGGQPIDEDSAALKKARAFYDRVLVGYGDDSVAQL
                     GGAHLACENISNVAAKLLEDARIGIAPLEKSTRYVRFDHKDAGGNYLFHREPEIMASR
                     HAGSYVEVMNLLFDTYSRQMEPMLASIAKMLPIEQVEVRDPTTGKTLPYADANKDDRL
                     RRWAETAYRATVRAHACDVLRSYLPAATLTNVGMFGAGQAFEYLLSKLYSSELSEAKA
                     IAAAMHGELNHLIPSFIKRAQSNEYLAQTSASAKNIAAKITKDSPRETRPVVLLDYDA
                     DAEEKVIAAILYAHTRHPLQQLRQIVAGMDHSERRRILQEQFAKRRHRRDKLSRAFEN
                     VYYTFDILGNLGLYRDLHRHRILTQERQDFTTVHGYDTPPEIEEAGFKSEFDECMRRA
                     AGLYEQIFEDLPGEAQYVVPFAYKIRWYMKMNLREALHMCELRTMPQGHPDYRFICQE
                     MWRKIQEVHP"
     gene            complement(1762..2922)
                     /locus_tag="VF182_20220"
     CDS             complement(1762..2922)
                     /locus_tag="VF182_20220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6439452.1"
                     /translation="MEKFDAAIVGAGPAGAAAAISLAGKGYRVALIDKEKFPRDKLCG
                     DFINPINWPILRDLGVENEVLSSEHDKVTAFRITACSGEHAQARLPALNRGPEFGLGL
                     SRRRFDLTLLRQAEHEGAVVLQGCRIKTLERDPQGWQLLLDQASGRGEIRAHVLVGAD
                     GRNSWVAHRLGLTSAAETQGRSVGFQIHLDAPNAVRGNIDIHIFPGGYAGLVGLGGNS
                     FNLCLAVDKQRLPREQQAEFLSESLLPRNPYLRELLLGSRRVGDIRATYPVYFKPRRC
                     VADGLLFVGDAARVNEPVSGEGIYFAMRSALLAAETIDEAFGASDFSASRLRSYERRC
                     RWEFKLRRGLNMLLRHLAYRPALLAPFVRYSARHGRLLDTLVHAICASQPAR"
     gene            complement(2961..>3791)
                     /locus_tag="VF182_20225"
     CDS             complement(2961..>3791)
                     /locus_tag="VF182_20225"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6439453.1"
                     /translation="EELSLGNRSGRDVKRSMAREMMDSPDNSPRELLEDDLRNLRIIN
                     RYLGNHRVVLGGLARLIEEQKLKRFSLLDVGTGSADIPTVIASWARRHHLTARLIGLE
                     PEPVTLRAAASQIRDYSEITLIRGDARALPLASGSVDFVLSSQMLHHFSEHDIINLLR
                     GWSRVARRGIIVCDLVRHPVAYHGIRLVTKVLTRNVMTRTDGPLSVQRAFTLEEWRDL
                     FERAGIGPFRLFPKFPFRQITIFSMLEIKSASRLREDQNDHESFAGSPAPCDLGPARM
                     "
BASE COUNT          837 a         1107 c         1064 g          783 t
ORIGIN      
        1 cggatgaact tcctggatct tgcgccacat ctcctggcag atgaagcggt aatccggatg
       61 gccctggggc atggtgcgca gctcgcacat gtgcaaggct tcgcgcagat tcattttcat
      121 gtaccagcga atcttgtacg cgaacgggac cacgtactgc gcttcgcctg gcagatcttc
      181 aaagatttgc tcgtacagcc ccgccgcgcg tctcatgcat tcgtcgaatt cggatttaaa
      241 ccccgcttcc tcaatctccg gcggcgtgtc gtagccgtgc acggtggtga agtcctggcg
      301 ctcctgggta agaattctat gccggtgcaa atcgcggtac agcccgagat tgccgagaat
      361 gtcgaaggtg taatagacat tctcgaaggc gcggctgagc ttgtcacggc ggtggcgacg
      421 cttggcaaac tgttcttgta gtattcgccg ccgctcggaa tgatccatcc cggccacgat
      481 ttggcgtagc tgctgtagcg gatgacgggt atgggcgtaa agaatcgccg cgatgacttt
      541 ttcttcggca tcggcgtcat aatccagcaa aaccaccggg cgggtttccc gcggcgaatc
      601 tttggtgatc ttagcggcga tgtttttcgc cgatgcagac gtctgtgcca gatactcgtt
      661 agattgagcg cgcttgatga atgagggtat caaatgattt agctcaccgt gcattgctgc
      721 cgcaattgct ttggcttcgc tcagctcgct ggaatagagc ttactcagga gatattcgaa
      781 ggcctggccg gcgccgaaca tgcccacgtt ggtcaaggtc gccgccggta gatagctacg
      841 taaaacgtcg caagcatggg cgcgcacggt ggcgcggtaa gccgtctccg cccagcggcg
      901 cagccggtcg tctttgttgg catcggcgta tggcagagtt ttccctgtgg tcggatcgcg
      961 cacttccact tgctcgatgg gcagcatttt ggcaatcgat gccagcatcg gctccatctg
     1021 ccgcgagtag gtgtcgaaca gcaggttcat cacttcgaca taagaaccag cgtggcgtga
     1081 tgccatgatc tccggctcgc ggtggaaaag ataatttccg ccggcgtcct tgtgatcgaa
     1141 acgcacgtag cgagtcgact tctccagcgg cgcaatgccg atgcgcgcat cttcgagaag
     1201 cttggcggcg acattggaaa tattttcgca agctagatga gcgccgccca attgggcgac
     1261 ggaatcatcg ccatagccga ccagcactcg atcataaaag gcccgggctt ttttcaaggc
     1321 cgcgctgtcc tcatctatcg gctggccgcc gagaagatct tgcaatccga gctcgggatc
     1381 gccgagaaac tcatccagga aagtacgccg caagctcttc gacgagcggc tgtagcgcga
     1441 aaacagggcc ccggccactt cttggggaag cttcaaacca aaaatcggcc gctcgaggtt
     1501 ggtgaagaaa ccgatcaaac gtttctgttc ttcagtggta aattcagcag tcattccgca
     1561 aatatactca taagccggga agctgtcatc tagaaagcgt tagctcgcgg caaagcaccc
     1621 aacgtgacaa aaccaaaaaa cctaagcgga taaagaggaa ttcaccgcag aggctcagag
     1681 cgcgcagaga tgttaagatt cgaagctctc ctccgcgtcc tccgcgcctc ggcggtaaca
     1741 ttttcttcga ttagacgaac ttcaacgggc cggctgcgac gcgcaaatcg cgtgaaccag
     1801 cgtatcaagc agccgtccgt gtctcgcaga ataacgcaca aacggggcga gcagcgcggg
     1861 ccgataggct aaatgccgca ggagcatgtt gagcccgcgg cgaagcttga actcccagcg
     1921 gcagcgtcgc tcataggaac gcaagcggct ggctgaaaag tcgctcgcgc caaaggcctc
     1981 atcgatggtc tcggcggcca gcagagcgct tctcatcgca aagtaaatcc cttcgccgct
     2041 caccggctca ttgacccgcg cggcatcgcc gacaaacaga aggccgtcgg ctacgcagcg
     2101 ccgcggtttg aaatagacgg gataagttgc ccggatatca cccactcggc ggctgcccag
     2161 gagcaattcc cgcagatagg gatttcgcgg caagagcgat tccgacaaga actctgcttg
     2221 ctgttcgcgc ggtagcctct gtttatctac cgccaggcac aggttgaaag agttgccgcc
     2281 aagcccgacc agtccggcgt agccgccggg gaaaatatgg atgtcgatgt tccctctaac
     2341 ggcatttgga gcatcgagat gaatttgaaa gcccaccgac cgcccttgcg tttctgccgc
     2401 gctcgtcaga cccagccggt gcgcaaccca ggaatttctg ccgtcagcac cgactagaac
     2461 atgcgcgcgg atctcgccac ggcccgacgc ctgatccagc aacagctgcc atccctgcgg
     2521 atcacgttct aaagttttaa ttctgcagcc ttgcaggaca actgcgccct cgtgctcggc
     2581 ttgccgcaat aaagtcagat cgaatctcct acggctcaga cccagcccga attctggccc
     2641 gcggttcagc gccggcaaac gggcctgcgc atgttcgccg gaacaggcgg tgatccgaaa
     2701 ggctgtgacc ttgtcgtgct cagaagaaag aacctcgttc tcgacgccga gatcgcgcag
     2761 aatcggccag ttgatcggat tgatgaaatc gccgcacaat ttgtcgcgtg gaaatttttc
     2821 tttatcgatt aacgccacgc ggtaaccttt gcccgccagg gatattgcag cggcagcccc
     2881 agcgggcccg gcgccgacta tagccgcatc gaacttttcc atttcaactg tatcgacgat
     2941 tggtcgcggg agagtccgga ttacattctg gccggaccaa gatcgcaagg agccggtgac
     3001 ccggcgaaac tctcatgatc attttgatct tcgcgcaggc ggcttgccga ctttatttcc
     3061 agcattgaaa aaattgttat ctgccgaaaa gggaatttcg gaaacagtcg aaacggaccg
     3121 attccggcac gctcgaacaa gtcgcgccat tcttccaagg taaatgcccg ttgcacggag
     3181 agcggcccat cggtgcgggt cataacgttg cgggtcaaga ctttggtgac cagacgaatg
     3241 ccatggtacg ccaccggatg tcgcaccaga tcgcaaacga taatcccccg gcgcgccaca
     3301 cgggaccagc ccctgagcaa attgatgatg tcatgttcgg aaaagtgatg aagcatctgc
     3361 gatgagagca cgaaatccac ggaacccgac gccaacggga gcgcccgggc atcgcctcgt
     3421 atcagcgtga tctccgaata gtcacgaatc tggctggccg ctgctcgcag ggtcaccggt
     3481 tctggctcaa gaccgatcag ccgagcggtg agatgatggc gccgcgccca tgaggcgatt
     3541 accgtcggga tatcggcgct tcccgtgccg acatccagca acgaaaaccg ctttaacttt
     3601 tgctcctcaa ttagccgcgc cagaccgcca agcacgactc ggtggttgcc gagatatcga
     3661 ttgatgattc gcaaattgcg cagatcatct tccagaagct cgcgcgggct attgtccggc
     3721 gagtccatca tctcgcgcgc catgctgcgc ttcacatctc tccccgagcg attaccaagg
     3781 ctgagttctt c
//
LOCUS       DASUVS010000379         4154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1475998, whole genome shotgun sequence.
ACCESSION   DASUVS010000379 DASUVS010000000
VERSION     DASUVS010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475998"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VF182_20230"
     CDS             complement(<1..623)
                     /locus_tag="VF182_20230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX6439454.1"
                     /translation="MLDPLYQAEPGDLTRLRGLLSSFIKIVVLVGRDFIENLVKLQAM
                     ALAFKTLLSIAPLLAVIFSILKGFGVHNRIEPVLAEALAPLGDKGQEVTSHLIGFVDR
                     MSAGALGSVGVVTLLITVLSLMGTIEQAFNTIWRVRFPRKLARRFSDYLSVILVGPVL
                     VFAALTITATLQSNAFVRNLLAIEPFGTVMLMLLRLVPYVILWGAFT"
     gene            complement(865..1509)
                     /locus_tag="VF182_20235"
     CDS             complement(865..1509)
                     /locus_tag="VF182_20235"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6439455.1"
                     /translation="MTASTILMPLMRGSIALLRATMRVEVLHGEYFADLQSRHIPILF
                     ALWHGRMFLVIDAHRGQGIVTMASKSDDGDLITGWLENNGYMVVRGSTTRGGSEALRR
                     MVYHMRAGRNGALTVDGPKGPVRIVQPGVVRLARLTGAWILPVSFSSSWPMFFKSWDR
                     HLVPRPFSRNFVSYGEPFPIGSDLSEEAALTKIAAAIDKTTEEVDKVAGICAPG"
     gene            1763..3157
                     /gene="glmU"
                     /locus_tag="VF182_20240"
     CDS             1763..3157
                     /gene="glmU"
                     /locus_tag="VF182_20240"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX6439456.1"
                     /translation="MNDIGVIVLAAGQGTRMKSEIPKVLHRLGGTPLFLHALRAAQKL
                     QPSRIAVIVGHGAAAVQQAYPGNDLCWVIQEKQLGTAHAVLCAEATFHRFTGDILILS
                     GDVPLIRESTLERLVDHHRRQQAAVTLLTATLDHPQGYGRIVRGDKQQMTRIVEEKDA
                     SDLQKQICEVNAGIYVVAAPFLFSGLARVNDQNRQREYYLPDIVAIALRENRTVANLQ
                     VDDPREMMGINTREELARMEKSLQEEINRKWMAKGVTLKDPMTIYIEEGAIIGKDTMI
                     GPNTHLCGTTVIGERCRIDGSAYLTDAHLGDEVHLRFSVVLTSCRVAKGAIIGPFAHL
                     RPGTELGRNVHIGNFVEAKEARVGDGTKANHLTYLGDVTIGRDSNIGAGTITCNYDGF
                     QKYKSKIGDRVQIGSDSTLVAPINLGDDVYVATATTVRHDVPDGALVFNDRQERLREG
                     WTEQKRIKMKGIKK"
     gene            3157..>4154
                     /gene="glmS"
                     /locus_tag="VF182_20245"
     CDS             3157..>4154
                     /gene="glmS"
                     /locus_tag="VF182_20245"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX6439457.1"
                     /translation="MCGIVGYIGSRDAAPLILESLRKLEYRGYDSAGIAVLNHGEMAI
                     RRCQGKLSNLATLLGRQPMSGAVGIGHTRWATHGRPSETNAHPHRAGDVVVVHNGIIE
                     NYLELKEQLSKRGTHFNSETDSEIVAHLVSEKINKGMDFLDAVRRTLREIRGSYALLF
                     LNRRDPQRLIVAKNSTPIVIGCGDGETFIASDIPALLDYTRKVTFLEDGEIGEVTVGS
                     YRLFNGTGKAIRRPVKEITWDAVAARKDGYAHFMLKEIHDQPRALADTFRGRISLKNG
                     DVSLEGIQLTQRQVKAIKRIHLVACGTAWHACLVGKFMLEEIAGIPAEVDYGSEFR"
BASE COUNT         1025 a         1106 c         1136 g          887 t
ORIGIN      
        1 aatgtaaaag ctccccatag gatgacatag ggaacaaggc ggagcagcat cagcatgact
       61 gttccgaagg gctcgatggc gagcaagtta cggacgaaag cattgctctg caatgtagcc
      121 gtaatcgtca acgccgcgaa caccagcacc gggccgacta gaatgacact caagtaatcg
      181 ctgaatcgcc gcgcgagctt tcttggaaat ctgacccgcc agatggtatt gaaggcctgt
      241 tcaatcgtgc ccatgagaga gagcacggtg atcaacagag tcaccacacc cacgctgccg
      301 agggctccgg cgctcatcct gtcaacaaaa ccgatcagat ggctagtgac ctcctggccc
      361 ttgtcaccta gcggagcaag tgcttcggcg agaacgggct cgatgcggtt atgtacccca
      421 aagcccttga ggattgaaaa gatcacggcg agcaggggtg caatcgaaag cagcgtttta
      481 aaggccaacg ccatggcctg gagcttgaca agattctcaa tgaaatctcg tccaactagg
      541 acaactattt tgatgaagga gctgagcagt cctctaagcc gagtgagatc gccgggttcg
      601 gcctgataga gggggtcgag caccctgctc ctaaaatctc ggatcgagtg tctctttcgc
      661 tgggtggttg tcttcatcgg gtttgatttc ttataacgcg ccggcagatc tcgaaaaagt
      721 ttccccaact gcacacaatg gctgcaacaa ttcagcgtgc gcgatttctc agccgcgcca
      781 agcccgggcg ggaaccgact gtcacacagg actcatttga acgcaaataa aaaagacttg
      841 attgcagttg ccgaactata acctctaacc aggcgcgcaa atccctgcga ccttatcgac
      901 ctcttctgtg gtcttatcga ttgccgcagc aatttttgtc agggccgctt cttccgatag
      961 gtccgaaccg attgggaatg gctctccgta tgacacgaag ttgcgtgaga aaggtctggg
     1021 aacgagatgg cgatcccacg atttaaaaaa catcggccat gagcttgaaa aactgacagg
     1081 tagaatccaa gcaccggtta atcgcgcgag ccgcacgact cctggctgca caatccgaac
     1141 cggtcccttg ggcccatcaa ctgtcaaggc tccgttgcga ccggcccgca tgtggtacac
     1201 catccggcgc aaagcttcac tcccgccgcg tgtggtagag cctcgaacca ccatataacc
     1261 gttattttca agccaaccgg tgatgagatc gccgtcgtca gacttcgaag ccatggtcac
     1321 tattccctgc ccgcggtgcg catcgatgac gagaaacatc cgtccatgcc atagtgcaaa
     1381 aagaattggg atatgacggc tctgcagatc ggcaaagtat tcgccgtgaa gcacttccac
     1441 ccgcatggtc gcgcgcagca gcgcgatgga gccacgcatc aacggcatta ggattgttga
     1501 tgctgtcata gttcttggct ttcaaaccga gattgtgcca aaacacgttt agtaaatcac
     1561 cggcgcatgc aagcaacccg ggtcagacat tgcgctttag ctcgtttgtc acgctcattg
     1621 attgcaccag gtaaagcacc gatggctaat aatagaggca atctagtcag tctaaaagtg
     1681 gtaaattccg attaacagcg cggtcctgct aagatcgctt actttgactg aaaagctact
     1741 ttcgctataa tccataacca tcatgaacga tatcggtgtc attgttttag ccgcgggaca
     1801 gggtacccgg atgaaatcgg agatccctaa ggtgctccat cgcctgggcg gcacgccgtt
     1861 atttctccac gccttacggg ctgcccaaaa attacaaccg tcgcggattg cggttatagt
     1921 cgggcatggc gcagccgccg tccagcaggc gtatccgggc aacgatctat gctgggtgat
     1981 tcaggaaaaa cagctcggta ccgcccacgc ggtgctttgc gccgaagcga catttcaccg
     2041 cttcacaggg gatattctga tcctgagcgg cgatgtgccc ttgattcgcg agagcacgtt
     2101 ggagcgactc gtcgatcatc accgcaggca acaagccgcg gtgacgttac tgaccgcaac
     2161 gctagaccat cctcagggtt acggccgcat cgtccgtggc gataagcaac agatgacccg
     2221 tattgttgaa gagaaagacg cttcagattt gcaaaaacag atttgtgaag tgaacgccgg
     2281 catctatgtg gttgccgcgc catttctttt ttcagggttg gcgcgcgtga atgatcagaa
     2341 tcgccaaagg gagtactatc tgccggatat cgtcgccatt gccttgcgcg aaaataggac
     2401 tgtggcaaac ctacaggtcg atgatcctcg tgaaatgatg gggatcaaca ccagggagga
     2461 gctcgcccgc atggagaaga gcttacagga agagatcaac cggaaatgga tggcaaaagg
     2521 cgtcacttta aaagacccca tgaccatcta catcgaagaa ggcgcgatca ttggcaaaga
     2581 cacgatgatc ggtccgaaca ctcatctctg cggcacgacc gtaatcggtg agcgctgccg
     2641 tattgacggc agcgcctatc tgaccgacgc gcatttgggc gacgaggttc atttgcgttt
     2701 ttccgtcgtc ttaaccagct gtcgagtcgc taagggcgcc atcattgggc cgttcgctca
     2761 tttgcgtccc ggcactgaat tgggccggaa tgttcacatc ggcaatttcg tggaggctaa
     2821 agaggccagg gtcggcgatg gcaccaaggc caatcatctt acctaccttg gtgatgtgac
     2881 gatcggccgc gatagcaata tcggcgctgg cacgatcacc tgcaactatg atggatttca
     2941 aaagtataaa agcaaaatcg gtgaccgcgt tcaaattggc agtgattcta ccttggtcgc
     3001 gccgatcaat ttgggggatg acgtctatgt cgccactgcg actacggttc gtcatgacgt
     3061 tcccgacgga gctctggtct tcaacgacag gcaagagcgg cttcgcgaag gctggaccga
     3121 gcaaaagcgc ataaagatga aaggaatcaa aaaataatgt gcggcattgt cggatatatc
     3181 ggcagtcgcg acgcggcgcc attgattttg gaaagccttc gaaagctcga gtatcggggt
     3241 tacgattcgg cgggcattgc ggttctcaac cacggcgaga tggcgattcg ccgctgtcaa
     3301 ggcaagctga gcaacctcgc aacgctgctt ggccgccaac cgatgtcggg ggcagtgggc
     3361 atcggtcata cccgctgggc aacccatggc cgtccatcag agaccaatgc tcacccgcat
     3421 cgagccgggg atgtggtcgt cgtccataac ggcatcatcg aaaactatct ggaactgaaa
     3481 gagcagctaa gcaagagagg aactcacttc aattcggaaa ccgacagcga aatagtcgcc
     3541 caccttgtca gtgaaaaaat taacaaagga atggacttcc tggatgcagt gcgccgaacc
     3601 ttgcgcgaaa tccgcggctc ctacgccttg ctgtttctta accgtcgcga tccgcagcgg
     3661 cttattgtcg ccaagaattc cacgccgatt gtgatcggtt gcggcgatgg cgaaacgttt
     3721 attgcctcgg acatccccgc gctgctcgac tacactcgca aagtgacatt cctggaagac
     3781 ggtgagatag gcgaagtaac cgtgggttcg taccgtctat ttaacggtac gggtaaagcc
     3841 attcggcggc cggtcaagga gatcacctgg gatgcggtgg cggcgcgcaa ggacggctat
     3901 gctcacttca tgctcaagga aattcacgat cagcccaggg ctttagcaga cacatttcgc
     3961 gggcgcatct cattgaagaa cggtgacgtt tctctcgagg ggattcaatt aacccagcgc
     4021 caggtcaagg cgatcaaaag aattcatctg gttgcctgcg gcacggcctg gcacgcctgc
     4081 ttagtgggta aatttatgct cgaagagatc gcgggaattc cggcggaggt cgattacggc
     4141 tcggaattcc gtta
//
LOCUS       DASUVS010000380        26382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_820778, whole genome shotgun sequence.
ACCESSION   DASUVS010000380 DASUVS010000000
VERSION     DASUVS010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 26382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26382
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820778"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..479
                     /locus_tag="VF182_20250"
     CDS             63..479
                     /locus_tag="VF182_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018497208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX6439458.1"
                     /translation="MEKVLASFAPYAYSLMRIVLGLLFVCHGGQKVFGWFGGMGGQPA
                     PLASMFGIAGLIELILGSLITIGFLTSYAAFIASGEMAVAYFIGHYPQSFWPLENQGE
                     PAVLFCFIFLYIATQGAGIWSVDGESTARRRRQTFR"
     gene            799..1725
                     /locus_tag="VF182_20255"
     CDS             799..1725
                     /locus_tag="VF182_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6439459.1"
                     /translation="MRISRRDFFKAGIAAGTALALPANSLFAQASALLQKKIPSSGES
                     IPIIGIGTARRYEEIQTAAEKAPLRATIQRFQESGGKVIDSSPSYGTAEAIVGELVDE
                     LNARQSLFLATKVSLRDSGREAGLKQIEQSFKRLRTDKIDLLAVHNLRDTQIQLGTMR
                     ELKQAGRIRYVGITTSFENQYPEFEQTIKKETLDFIQVDYALDNRNAGERIIPLAGER
                     GMAVMINLPFGRGRLFNAVQGKKLPEWAAEIDCSSWAQFFLKYIVSHPAVTCAVPGMA
                     KPEYVSDNLGAARGRLPDAAMRRRMENFIDAV"
     gene            1766..3109
                     /locus_tag="VF182_20260"
     CDS             1766..3109
                     /locus_tag="VF182_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6439460.1"
                     /translation="MGDSSTAAKPRDVGGPIYRLLRRFLDVRPAETRALGWSWLYIFS
                     VLSSYYILRPVRDDMGVAGGIENLPWLFTGTLAGMIAVNPPFAALVARLPRVRFIAIA
                     YRFFISNLLIFAVLLNAASAEQNIWLGRVFFIWTSIFNLFVVSVFWALMVDVYDSEQS
                     KRLFGFIAAGATLGGIAGSSITASLAKEVSPVYLLFISAALIELAVFGVRRLSKLSQA
                     LHRQPAIRSDEAPIGGSVFSGITHALKSPYLINVSVYVLLFTITSTFLYFQQAEIARQ
                     SFADRGARTAFFARIDLWVNILTLGAQLFLTHRLLRSVGVAATLAILPALSILGFGTL
                     ASMPTIAVLVAYQVLRRAGNFAFARPTREVLFTVLPREDKYKAKSFIDTVVYRLGDQL
                     GAWSYAGLGLLGFGMTGIAVVAVPISCAWLVNGWWLGRKQEKMVKKPAEVASGAI"
     gene            3411..4475
                     /locus_tag="VF182_20265"
     CDS             3411..4475
                     /locus_tag="VF182_20265"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6439461.1"
                     /translation="MAHIRSSLFIVTVFLFLLSSFAAARAITLPELTAQVEKLSRAER
                     EALLVKGGKEEKEVMFYATTPVNQVAVLKKAFSARYPFVDLKHYYSPRQGILNKTVSE
                     YRGKANITDVVMTDVSYGTILIKEGVSHPYTTPDVQRYVRGSYDPKGHWYTMYMLTTA
                     LVYNRNLVKPGEAPKTYQDLLDPKWKGRMMFDPEAAFIMAAMEEVWGKEKAREYLARL
                     AKQDLIFRRGTTLTSQLIAAGEHPIAIAVNAETTAEMRDKGAPLGFSVLPPRIIKPNG
                     FFVAKNAPHPHAALLFADWSLSEEGQKVLAVDLGKGVAMKGIPAKYKEFQGEPDYVVG
                     PEFGNQLKQHMTEFQKIFGM"
     gene            4968..5975
                     /locus_tag="VF182_20270"
     CDS             4968..5975
                     /locus_tag="VF182_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006759793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6439462.1"
                     /translation="MTAPILARSMIGNSLEIWKLKTYLRKVAITDSHVLITGETGTGK
                     ELAAEYIHQHSTRRAKPLVTINCAALPDGLLESELFGYERGAFTGALSAYPGKLKHAD
                     GGTVLFDEIGEMSPYAQAKILRVIESKEVYPLGGRRSIPVNIRIMAATNRDLDHEMAS
                     NGFRKDLYFRLNVARIKLPPLRERKEDIPLLIDHFAQKFSEVFGRQIEGFTDEALELL
                     KRSDWPGNIRELMNLIERIFIDPPTGKISAADLPDSIRSLKPCRSDAIPEERELLLSA
                     LSQTNWNKSQAAERLHWSRMTLYRKMAKHHIQETTPEQLQRALAVRTSRNNLGAGVTK
                     V"
     gene            6314..6559
                     /locus_tag="VF182_20275"
     CDS             6314..6559
                     /locus_tag="VF182_20275"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEX6439463.1"
                     /translation="MAQERKSGQSVDPPEGRYANYFKIGYNAFELIIDCGQCYADDAA
                     PQLHSRIITSPAYGKALLKTLSDSLEEYEKNYGRAGE"
     gene            6751..7350
                     /locus_tag="VF182_20280"
     CDS             6751..7350
                     /locus_tag="VF182_20280"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEX6439464.1"
                     /translation="MYTALRATSRTLAKFLETRFQADPLLSGFFGGGMVVSLATPQEM
                     NEKPAEGLSVWLYRIIRDEQRLNDPPVRISPTELRPPPLPVRLHYLMTPVTNEQTGDP
                     ETEQLILGKVLQLFHSHPVLAGADLQAEFTGTEVELRVRLEPLMLEEITRIWEALEGS
                     YQLSVSYEISVVNIMPELEPEQVSPVMISMPEHGLIVSS"
     gene            7372..8547
                     /locus_tag="VF182_20285"
     CDS             7372..8547
                     /locus_tag="VF182_20285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439465.1"
                     /translation="MSRRAIQVDHRIILYSPDDPASEVDRFQAVLKVRVTDELTGAAP
                     NSQSVLQVKERRFFSRLGNDGLGGLVAIPRQVFPALQARNYPLHLTIGAARYQRRVVQ
                     KDIPQDFNFPGAFTPQELDIALHRAPVFIAGRTARLINNATTPLPDAQITVTGIWRTA
                     PPANVSVPPDPANIIALAPPLYADRAPLTQSVAARDLTPVSGADKTLMDDVSASANPI
                     RISDRSGLAVGDILMIDADDPDRVEFIAIKNLSTTAPADQPAVVSLDYPVIFSHRRNT
                     NVRPINPQPAGVSQTITIEAWAGDACVFLNGIGALVGAHEIEITGGPGPVEYHRVKTF
                     SATSDTEGYYRLPPLSRVAQLEIHAEKMIGAQTFHATTVFRPVYQQRDNRLDLILAA"
     gene            8614..11832
                     /locus_tag="VF182_20290"
     CDS             8614..11832
                     /locus_tag="VF182_20290"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEX6439466.1"
                     /translation="MPEYLAPAVYVEEIDSGSKPIEGVSTSTAGMIGLTERGPANVPI
                     LVTSYGEYVRWFGDRLNHADFGDHCYLPHAVEGFFTNGGKRVFITRVEASGAARASFE
                     LHDQGAANSGFTLLLRGAPVDTGTSGHTPIYILDINTPGAGALADGDRIRVGDDSDAE
                     YRIIDKIEPAASTTHVPLGFPLSRAHASGVTVHQINRVTRAVPSTATALALANEAHAS
                     DNAVVVSGAAADIATLAAGQLLEIGDPFFNEHRFIRAAVIDPADDKIARVTLDGPLAL
                     EHAAAADRVRRLDIDLTNTAVTVDDDTLSAAALPNDRVVYVTDRNNNFDDRTTLIILD
                     INSSATREARRIGALSMVTLTTGLYAAIPAGSLMERIDLGDDAAVTAKTLTAAARAGT
                     AVIALDNRVGLEVKRILRIGAAPDDEYLTIAALPNRSPGSAAPDAGTVVLSHPLNRDH
                     APGSPARLQTDPVVNTARPATTLLLDAALGSASLPVTDGDGYAQDEFVRLTPAGGRPL
                     YHRLAGNAASLDARLVNLSVPLSRAHQAGAAVAERAPLLSVQALDSGDWGNRLRVSVQ
                     DEPAGLVPRTAVSSVIDANHIRLTSAAGVEAGTILEVFDPAGGVTVGDLLKVTEVNRA
                     ANFTIALGGAGISASQSAPGLAVRSREFRLSVYLFRQPDPAIPLRNETILDSEVFRYL
                     SMDPRHSRYVQDIIGDINGALRISDRRPEGESRYIRVHDMAQDLSEPDRTTTLASIRR
                     GPETLVDVLPDGRRRPARHALGDLVRGTDSVGTLTEDDYVGQDNQDPEARTGLHSLRN
                     IEEISIVACPGRTTAVMQNALIAHCELMRYRVAVLDGPRPPQDTLADVQTQRQQYDTK
                     YAALYHPWLLIPDPYPLNLSQIADYPIPPSGHIVGIYARTDIERGVHKAPANEVVRGI
                     IGLQRIVNKEQHDILNPYPVNINVIRDFRDANRGIRVYGGRIISGDPDWKYLNMRRLM
                     IFIEASIDRGLQWVVFEPNKEDLWARVRRSISNFLTLVWRNGGLEGTKVEEAYFVKCD
                     RTTMTQTDIDNGRLICVVGVALVKPAEFVIVRIGLWTAHAED"
     gene            11854..12297
                     /locus_tag="VF182_20295"
     CDS             11854..12297
                     /locus_tag="VF182_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX6439467.1"
                     /translation="MATGERRDPFRAFNFQLEIDNIARGAFSECSGLTAEGDAVDYRE
                     GTDLQPNVRKLVGLRKYTNIVLKRGYTQEDTSLWDWYKNIHNGVPDRRNVTIILMNDE
                     RQPVMRWHAENAWINKIEGPSFKASGNEVAIESVELVHEGLTIEV"
     gene            12312..14312
                     /locus_tag="VF182_20300"
     CDS             12312..14312
                     /locus_tag="VF182_20300"
                     /inference="COORDINATES: protein motif:HMM:NF037565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEX6439468.1"
                     /translation="MVQLPQTPGVYYERVDASAPAIALLRTDIAGFVGIATRGPLHQP
                     LPVQSWRQFQAYFGDFTGAGYLAYAVRAFFENGGQRCWVVRVASETAATAAVTLQSES
                     RVGVWTISASSPGVWGNDLDLVIGEKHRAQTLTVPEISAPEFSTVLSTTGLGRATLVR
                     LSQGATRIHKVVSDVDPVSHRLLWINPKSEARLPYDSVLSGFDAGHPILIESIEYTLS
                     VRERGRLIRVYEGLSLVPEHLRYGPALLGQFKLPRFSGQPVNLPAAPEPVTITELRAL
                     PDADLESIATIADQIMPLTGGADGLAQLRAYDFIGEDLDPLDSDEVRIRKARGLRTLE
                     DVCEIAIVAVPDIHIQPKAAPRFSPPPPCVPDPCLPVNLPPAVPRTPSLGDLPPVFPD
                     EAIYQVQSALIQHCEKLSDRIALLDPPFSASRDDKLGIGAVRAWRSRFDSKYAAFYYP
                     WLRVVDPLRSPMSLVREIPPTGHVAGQYAQNDSQFGVHKAPANAPLIWAEDVTVFVDD
                     AVHGILNGIGINAIRPLPGRGLRISGARTVSSDPDWRYVNVRRLMMMIEKAIETAIQW
                     AVFEPNSTLTRTKLNLTLTSFLLSLWQRGALMGASAEDAFFVRCDDENNPLEERDHGR
                     LLTTIGVAPSRPFEFIILRVGRAHNQFEISEASSGASGLEVF"
     gene            14312..14827
                     /locus_tag="VF182_20305"
     CDS             14312..14827
                     /locus_tag="VF182_20305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007300289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX6439469.1"
                     /translation="MALNPTGRRDDPVLNHNFVVSLLDSSSASSLFGSVSMSAIFDVA
                     LGGFSECGGLEMSLQVQEFQEGGNNGRLLKFPTRVTWSNITLKKGVSSGTALWDWHYA
                     FTAGRGSRRDGVIVLLNDRQLPNNIWYFRRGLPVKYTGPALNATQSGVAIESIEIAHE
                     GLSQVPTGLAP"
     gene            14846..15043
                     /locus_tag="VF182_20310"
     CDS             14846..15043
                     /locus_tag="VF182_20310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439470.1"
                     /translation="MEVQIQTLQSTVQAFDGDVALSPQTMDKIVRAVLQAVNDEQAHR
                     QRVRAEQRITTGVSHELEERD"
     gene            15048..15872
                     /locus_tag="VF182_20315"
     CDS             15048..15872
                     /locus_tag="VF182_20315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX6439471.1"
                     /translation="MEQVAKAVLRVEWDFPRNPEMIEVQYNPTELSWDKGAQIAEITI
                     PGLDAPLQQFVRGQAEKLNLELFFDSTDHGMGKGAVSVTTLTDRIYQLIKIEPSRHAP
                     PVCTFIWNDAFPGSSLEGNAGSAASLAIGGSFGVAAGVVSSAAGVAVSAAASAVAGIA
                     RAASGNQRRNGFRCIVESVKQKFTLFSSEGVPLRAALSVALREYKTLNDQLAQLNLTS
                     PDRTHSHVTQNGDSLSRIAAQYYRSAGDWRLIAEANGVEDPRRLVVGALLQIPPTQ"
     gene            15883..17136
                     /locus_tag="VF182_20320"
     CDS             15883..17136
                     /locus_tag="VF182_20320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439472.1"
                     /translation="MPVVTLGDQSKLQGNFYVPRFEVKIAGANLPQNVLRDVLQLTYS
                     DNVKELDSFEFTVNNWDAEKHEFKYVGSETTDSLSSNPLHRLFNPSRHEVEVSMGYEN
                     NLTLMMKGSFTTMEPTFPNSGGPTLTVRGLNILHKLRSGQYSYAWMPDEKHPNGWKES
                     EIAKSLRKVPDLDHKEEGKNKEAKKPFPLEVEIDENALSKEHPITYVAQDGQYDIDFL
                     LSRARRIGYVVFVKEEEREKGRVTKERRLYFGPSEANHPGLRPLTFELKWGVSLIDFK
                     PTLTTANQVKTVVVQGTDRSTKKPITGKASLDEPRFGLNRDLIKLLEASDARDERVVD
                     RPVATQQEADELARAILRERSKDILKANGTCVGLPELRAGQRIKIGGLGARFSGEYFV
                     TDTVHTINDSGYITRFGARREHQGK"
     gene            17147..17674
                     /locus_tag="VF182_20325"
     CDS             17147..17674
                     /locus_tag="VF182_20325"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEX6439473.1"
                     /translation="MKHYPGVVTAIVKNLTDPNGQGRIELQFPWLSDSLRSYWAPVAA
                     PLAGKDRGMFFMPEVDDEVLVAFEHGSFDHPYIVGYLWNGVDMPPETDLKNRVIKTPG
                     GHQLRFEDADGAKKVILKSNGGHQVEIDDAAETITIKTNSGNQLIVLNDAAKSVTVRG
                     GGRRVDMVGGQIKMT"
     gene            17691..18422
                     /locus_tag="VF182_20330"
     CDS             17691..18422
                     /locus_tag="VF182_20330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439474.1"
                     /translation="MPQVVTTNTQILCAHFSTAHKNPPLAPLWTIDGGTVLVEGDSGT
                     FPSCPSTFQCGGYQLRSMGLNATQVTGKKVILVTDFNQTSSGLPITMIETHPVFDETT
                     PAAIPDGQPAPPLPPEMTDHVKPVVISSLPALAFNKTSMTPPSVLVTFTLTSAHPMRW
                     ILTQIDEPGPQPGTHDRTNAPQPPGMTVNPSGGVWNISPLNINLTMTAPFMASLLPGD
                     HRFFLTGVTQRGLSNFAEVLLTVAV"
     gene            18438..18854
                     /locus_tag="VF182_20335"
     CDS             18438..18854
                     /locus_tag="VF182_20335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEX6439475.1"
                     /translation="MNGTQRSSQREFLGVGWKFPLQVTPGGQIAQARYEQRIEESIYL
                     ILSTSKGERVMLPEFGCGIHDLVFAPNNSTTIAVVVQTVREALVAFEPRIDVLDISAE
                     SAPEEPNLLLIRINYRIRANNARGNLVYPFYIQESA"
     gene            18858..20819
                     /locus_tag="VF182_20340"
     CDS             18858..20819
                     /locus_tag="VF182_20340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEX6439476.1"
                     /translation="MPLENALPRIDDRNYDSLIAEVRTRIARYTPEWTPVWTDVNDND
                     PGITLAQVFAWLTEMLAYRMNLVPELNYIKFLQLIGIELHAAEPAQAEVTFPVQSGFA
                     EAYVIVPPRTQLTADAPDGSAPITFETDRALIAVRAQLTAVLAYDGYAYTSVTADNQE
                     AVQGFQPFGPMANDGSALLLGFDDRGPFPALELNLAIVTPDNSAAAAFQCGLPDRPGF
                     ASATLRWEFWNGSDWQRLSLLKDETRAFTRSGHVFLKTPNKSLQPVVIAPESRPLYWI
                     RARVERSQYERVPKLLTIRTNTIAVRQAETVRDEVLGGSNGRRDQVFRLANVPVLRET
                     LRLEIDEGSGPEIWTRVDDFFGSGPADPHYMLNRTTGEIRFGDGRNGNIPVANPANPE
                     GNIVAREYRFGGGRQGNVGAGAIKTLTTSIDGIDSNAVANLLAADSGRDEETLDEAKK
                     RAPHAIRSRYRAVTAEDFELLAMEASSIKRAKALPLYHPDFPGVKVPGVVTIIVVPDG
                     DGPSPTPSEGTLRSVCAYLDQRRLLTTELYVIKPTYQRVQIRAEIVVSDDADLGEVQQ
                     QMEQSLLDYFHPLKGGEDGLGWPFGGTIYYSRINQRCFTIPGVASITSLIIVLDGEEI
                     PPCTDVTINEAGLLFATEHQIDVSYRFES"
     gene            20824..23064
                     /locus_tag="VF182_20345"
     CDS             20824..23064
                     /locus_tag="VF182_20345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX6439477.1"
                     /translation="MMTASQLPPIPRPPHDPTSLLLNWRTGWRAAELQQLSAERSLLM
                     APAPETQRSLTEISGSFGGLMAPANVACGPDSGVYLLDAETAQLKRFDPCACRFTQVP
                     CFGGTGSGPRQLKSPHGIAICAGNLFVCDTGNHRLSVFALHGFVLRGFWQPPPAAYKG
                     SHPTLANLWEPFDVTFDGVGRAYVTDGANGCIHRFTAAGQWENCFTGFGQATWIAIDC
                     RDRSYVIVAGSPAQVVRSDPDGNRIAIDSMPGELMSLFPRNSISVDAAGLLHLGAFCA
                     GDAPMNCQNPQPTLKCPRGQTPERGVFDLRGNTVDCFVSTPASYVTQATYISAALDSE
                     FYRCQWHRIILRGEIPAGSRVTVSTYTAEAELADDDIENLGEVWQTNQTATEMNKGDW
                     DCLVRSSGGRFLWLRLEFRGNGRVTPRLDSVELEFPRISLRRYLPGVFGREAVSADFT
                     DRFLSLFDTTFRSIEANLDRGARYFDPLSAPAERDPKTGADFLSWLGSWIGLILDRHW
                     PEARRRKFLKHAGRLYDLRGTREGLWRELLLFLDLDRDECCRADQPSDRCQARPANCA
                     PPKPSECGWRPPPLILEHFKLRRWLFLGAGRLGDQAVLWGKRIVNRTQLDDTARVERT
                     QLLTAQDPLRDPFHVYAYKFSVFVPACFRASNTEAKALENILRNGRPAGTQAQLEYVE
                     PRFRIGFQSMIGFDSVVGRYPSGVTLNEMQLGRATVLTAPPHKQGAPSLTVGEQSRIG
                     TTTRLE"
     gene            23110..25641
                     /locus_tag="VF182_20350"
     CDS             23110..25641
                     /locus_tag="VF182_20350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439478.1"
                     /translation="MTHAQNVVAQLACPACNYGPFTRNNYFTGKLLVERDFTDESRFH
                     IEKLRHHEQLLHGWGVVCGLKVKAHPNEACRDRFVCVEPGSAVDCCGHDIIVAEEECV
                     DFSQFPEIKALKSNNDKDPHTLQICARFRECPTEDIPVLYDDCGCDDTKCAPNRILES
                     YEIDIMLDPPKQPQDLHTPEFSWENSIALAHAAAAVVHDASHRLYVLTADDPATVYQI
                     STDNHATITARTLTKKAIALSVSNDGKHLYVVVEPTSPATLRRLQVLDTTQPGMPDFN
                     TDPLDLPNSFGSDVTLAVTPDGRLISLVSSSGDVLRWPADLDTNPVPAAPELVKNLGT
                     GVTGLTLSSDGKKAFVLGSGNKIQALDLGAATVTPITVLPAAAKPSSMALVTSTAPDM
                     LAVADQTNLFLHLVELLPAAALIGSVKLDHPPVGLVAAPGGHWAYVLERDTDSFIQSV
                     SLDGLVQHLGVTLGTAFMIGDDSRQIVMSASGKRLYIPYVDDISQTAAGGVAIVEVTE
                     GSCAEILWRHLDGCPHCDLPDCVVLATIENYHLEDRVEEQTDPPADAAQDAINKIARI
                     DNRKGRRLLPSTQVLTELVECLLEHGVGGAGTQGPPGAPGAKGEKGDKGGKGDKGEKG
                     DQGLKGDKGDSGSGLELGLVRIKALSWTHNTPSHLIGVKMLNQTQVPGFVIGFTDKVH
                     VRTQDQSGALVGLIDASHIFQVLVASDLEQNKELGMFCRCPIAGDIVPVDYTETNGLI
                     TTATEVQDLDAPGIAFILDRSRSRRAGEIISQTPPFDKPPFNDIWIRLRGDFVLDTKD
                     RAIDAEFVRAKLPTGDRPDPGGLPLDKQPGIQGGVFESWFTLLPG"
     gene            25681..>26382
                     /locus_tag="VF182_20355"
     CDS             25681..>26382
                     /locus_tag="VF182_20355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439479.1"
                     /translation="MALATTTTRTPTVARCPACGRLECLCRPRFFAGQLLTEEDLNRL
                     DHYIVEKNKLHNRYLHGWGVVCGLEVFCHPCEGQVTVTSGYALSPCGDDIVLCANDVV
                     NVCDLIQRCRERERLDCEPPRPATTAECQDVQEQWVLTVCFTEKSARGVTALRGSSES
                     ACCSSCSCGGSSSCGCEESGMSRKQAAPATRSARSLSTDARSGRDLCEPTVTCESHYF
                     SLYKASAADPRPEQAL"
BASE COUNT         5597 a         7560 c         7697 g         5528 t
ORIGIN      
        1 ggtttagctt cttggcacgt caccgttagt gcattcagtt cgtcactgag gaggtcctgg
       61 tcatggagaa agttcttgcg tcatttgcgc cgtatgccta ctcccttatg cggattgtcc
      121 tcggattatt gtttgtttgt catggcgggc aaaaggtttt tggctggttt ggcggtatgg
      181 gcggacagcc agcgccgctg gcgtccatgt ttgggatcgc aggattgatc gagctgattc
      241 tcggctcgct tatcaccata ggatttctga ccagctatgc ggcatttatt gccagcggcg
      301 aaatggccgt cgcttacttc atcggccatt atccgcaaag tttttggccg ttggagaatc
      361 aaggcgagcc ggccgtgctc ttctgcttta tttttttgta tatcgcgact cagggggcgg
      421 gaatctggag cgtcgatggc gagtcgaccg cccgcaggcg gcgccagacc tttagataag
      481 cgtctagaat cagaatcgaa gctttactta attagtaagg ttccgccggg tcgcgttgcc
      541 tatcgattgg caaagagttc aaagttcgct tcgaagtgct ccgcaacgga tcgcagcctg
      601 gcagcgggtg aactaacaga ctgctgaaaa ttctgattgc aggctggtta aaaagatcca
      661 aaggcgaggc gggcgaaaat acaaggatga aagcggaaat ctgaaagcgg aattcacttt
      721 tcagttttca taattcatct ttccaaaagc atatgggtct tttccgcaac ctgccaaaga
      781 cggatcagga gattgctgat gcgcatcagt cgtcgtgact ttttcaaggc cgggattgct
      841 gcgggaacag cgctggcgct gccggcaaac agcctattcg cccaagccag cgcactgttg
      901 cagaagaaaa tcccctcaag cggcgagagt attccgatca tcggtattgg caccgcccgg
      961 cgctacgagg aaatacagac ggccgccgaa aaagcgcctc tcagggccac gattcagcga
     1021 ttccaggaat cgggcgggaa ggtgatcgat agctcgccta gctatggtac ggcggaggcc
     1081 atcgtgggcg agctcgtcga tgagctcaat gcgcgacaat ccctgttctt ggcaaccaag
     1141 gtaagtttgc gcgacagcgg gcgggaggcc ggactcaaac agatcgaaca gtccttcaag
     1201 cgcctgcgca cggacaagat cgatttgctt gcggtgcata atttacgcga tacccaaatc
     1261 cagctaggta ccatgcggga attgaaacag gccgggcgaa ttcgctatgt cgggatcact
     1321 acttctttcg agaaccagta tccggagttc gaacagacga taaaaaaaga gactctcgac
     1381 tttattcagg tcgactacgc tctcgacaac cgcaacgccg gcgaacggat cataccgctg
     1441 gccggcgagc ggggcatggc cgtcatgatc aatctgccat tcgggcgcgg ccgattgttc
     1501 aatgcggtgc agggcaaaaa attgccggaa tgggcggcgg aaatcgattg cagctcatgg
     1561 gcgcaatttt ttctcaaata catcgtttca catcccgccg tgacttgcgc ggtgcccggc
     1621 atggctaagc ccgagtatgt cagcgataac ttgggcgcgg cgcgtggtcg tctgcccgat
     1681 gcggcgatgc gccgccgcat ggaaaacttt atcgatgcgg tgtaatcgac agccagccaa
     1741 ttgatacgtc acagcggagg cggtgatggg tgattcctcg acggctgcca aaccgcgaga
     1801 tgttggcggt ccgatttacc ggctcctcag gcggtttctc gatgtccggc ccgctgaaac
     1861 tcgagccctg gggtggtcat ggctgtacat attttcggtg ctctcctctt attatattct
     1921 ccggccggtg cgcgacgaca tgggcgttgc cggcggcatt gaaaacctgc cgtggctttt
     1981 taccggaact ctggccggta tgatcgcggt caatccgccg tttgcggcgc tggtggctcg
     2041 gctgccgcgg gttcgcttca tcgccatcgc ttatcggttc ttcatcagca atttgttaat
     2101 tttcgcggtt ctgctcaatg ccgccagcgc tgaacagaac atctggctgg gccgcgtgtt
     2161 ttttatctgg acatcgattt tcaatctttt tgtcgtctcg gttttctggg ctttgatggt
     2221 cgatgtgtac gattccgagc agagcaaacg gctcttcggt tttatcgccg ccggcgccac
     2281 tcttggaggc atcgccggat cgagcataac cgcttcgttg gccaaagaag tatcgcctgt
     2341 ttatctgctg ttcatttcag ccgcgctgat tgaactcgcg gtcttcggag tgcggcgatt
     2401 atcgaagctg tcccaggcgt tgcatcggca accggcgatc cgttccgatg aggcgccgat
     2461 tggcggctcg gtcttttcgg gcattactca tgcgctcaag tcgccatatt tgatcaacgt
     2521 aagtgtttat gtgctgctgt ttaccatcac gtcgaccttt ctttactttc agcaggcgga
     2581 aatcgcccgg caaagctttg ctgatcgagg cgcgcgcaca gcctttttcg cccgcatcga
     2641 tttatgggtg aatattttga cgctgggggc gcagctgttc ctgacccatc gccttttgcg
     2701 cagcgtcgga gtcgcggcaa ccttggcgat cctgccggcg ttgagtattt tgggtttcgg
     2761 cacgctggcg tcaatgccga cgattgcggt gctggttgct tatcaggtgc tgcgccgcgc
     2821 cggaaacttc gcatttgcgc gtcccacccg agaggtgctt ttcacagtgc ttcctcgtga
     2881 agacaagtac aaggcgaaaa gtttcatcga taccgtagtg tatcggctcg gcgaccagct
     2941 tggcgcctgg tcctacgctg gtttaggact gctgggattc ggcatgaccg ggatcgctgt
     3001 agtcgcggtg ccgatctcgt gtgcctggct ggtaaacggc tggtggctcg gccgcaagca
     3061 ggaaaagatg gtcaagaaac ctgccgaggt agcatcgggc gccatctgat cgtcggagca
     3121 aaagatcatc cgccgtaatc gtttatattc tcgccagcct cagccgatta aagtcatctg
     3181 tttatcgttc tcgggcataa cttgcttcgc aggaattagt agtctatgta agttgcgaca
     3241 agggagtttt ttccgccgaa ctatcagttc catgcttaga tgcatgcatc gttaaggcac
     3301 gccgtttgag ttcatcgaat cttgatgcgc gatgcatctc aggtcttttc acaccttgta
     3361 cgggcaggtg ccagcagcct atcgctatca ggaatcacgt cggagggatg atggctcaca
     3421 tcagatcaag tttattcatc gttactgttt tcctgtttct gcttagctcg ttcgcggccg
     3481 ctcgcgctat caccttgccg gagctgaccg ctcaggtcga aaagctgagt cgcgccgagc
     3541 gtgaagcgct cctcgtcaaa ggcggcaaag aagaaaaaga ggttatgttt tacgccacca
     3601 cgccggtcaa tcaggtggcg gtgctaaaga aagctttcag cgcgcgctat ccatttgtcg
     3661 atttgaagca ttactattcg ccgcggcagg gaattctcaa caagaccgtc agcgaatacc
     3721 gcggcaaagc taatatcacg gacgtggtca tgaccgatgt cagctacggc acgatcctga
     3781 tcaaagaagg cgtttcgcat ccgtatacca cccctgatgt gcagcgttac gtgcgcggct
     3841 cctatgatcc gaaggggcac tggtacacga tgtacatgtt gaccaccgcg ctggtctata
     3901 accgcaacct ggtcaaaccc ggcgaagcgc ccaagactta tcaggatctt ctcgatccga
     3961 aatggaaggg cagaatgatg ttcgatcccg aggcagcttt catcatggcg gcgatggaag
     4021 aagtctgggg aaaagaaaaa gcgcgcgagt atctggccag gttagccaag caggatctga
     4081 tcttcaggcg cggcacgact ttgacctcgc aactgatcgc ggccggcgag cacccgattg
     4141 cgatcgcggt aaacgccgaa accaccgcgg agatgcgcga caaaggcgcg ccgcttggtt
     4201 tcagcgtgct gccaccgaga atcatcaaac caaacggatt tttcgtcgcc aagaatgcgc
     4261 cgcatcccca cgccgctctg ctctttgcgg attggtcatt gtcggaggaa ggacaaaagg
     4321 ttctcgctgt ggatttgggt aaaggggtcg cgatgaaagg catcccggca aagtacaaag
     4381 agttccaggg cgagcccgat tatgtcgtcg gccccgagtt cggtaatcag ttaaagcagc
     4441 acatgaccga gtttcagaag atctttggca tgtgaacggc tgatgaaaaa ggcacgtccg
     4501 cgtcgttgcc tgcttcgctt cggcgtacta ttcggagtag attttagctc agtgagcggc
     4561 gcgccttgct ttttgatctg acaataacca cacctgcttc acgctccgat tctggagatt
     4621 tgacattttc catcagctgg aacccgctcc ttcatccgta aacgtttgtt acgcgcagtg
     4681 tgtctaaagt tgcgccggcg tttttgagaa aaatgatctt cggtagaaaa attccgaagt
     4741 gttcgcgctt cgaactgtac cctcgacttc cgcattcggt ctcgcatttg ctttaacgaa
     4801 cgttaagatc acataacggt attaacgatc gacaaaaact gcttgaccga gtgataaaga
     4861 ctgtactaag tcaaaaaata atccggcgta acaaggtcta gccgctctcg gatccgctgc
     4921 gctcactgcg cgtcgggata ctcaaacatt accccggagc tccagatatg acggcaccca
     4981 tcctagcgag atcaatgata gggaacagcc tggagatctg gaagctcaaa acatacctgc
     5041 gaaaagtggc tataaccgac agccacgtgc tgattaccgg cgagaccggg accgggaagg
     5101 agcttgccgc tgagtatatc caccagcaca gcacgcggcg cgctaagccg ctggtcacaa
     5161 tcaactgcgc cgctctcccg gatggtctgt tggaaagcga attgtttggt tacgaaagag
     5221 gcgccttcac cggcgccctt tccgcttatc cgggaaagtt gaaacatgcc gatggcggca
     5281 cggtgctgtt cgacgaaatc ggcgagatga gcccctacgc tcaagccaaa atccttcggg
     5341 taatagagag caaggaggtc tatccgctcg gcggccgccg cagcataccc gtaaacatac
     5401 gaatcatggc ggcgaccaac cgcgacctgg atcacgagat ggcaagcaac gggtttcgca
     5461 aggatcttta tttccgtctt aatgtcgcca ggatcaagct gccgccactg cgtgagcgta
     5521 aggaagatat cccgctgctg attgatcatt ttgcgcagaa atttagtgaa gtattcggcc
     5581 ggcaaatcga aggcttcacg gatgaagcgc tggagttgtt aaaaaggagc gattggccgg
     5641 gtaatattcg tgagctaatg aatttgatcg agcgcatttt tatcgacccg ccgacgggca
     5701 agatcagcgc cgccgacttg ccggattcaa tacgatctct caaaccttgc cgcagtgacg
     5761 ctatccctga agagcgtgag ctgttgttat cggcactgtc ccaaaccaat tggaacaaga
     5821 gccaggcggc agagcgactg cattggtcgc gcatgactct ctaccgtaag atggcgaagc
     5881 atcacattca ggagactaca cccgaacagt tacaacgcgc tcttgccgta agaacgtcgc
     5941 gtaacaactt aggcgctggt gttacaaagg tgtaacagcg agctgttaca ccgaggtaga
     6001 tagagaatcg aattgcctta gaggttttgc ttctcgctga tttgttttca tcatcacgat
     6061 ttgccgctct cactactgac tcgccccatc atacgagcgc tcgcctgatt acaaccacgc
     6121 tgtaacattt cgcgttacac cttctttatc gcacttccga ctgggccatc gaaactcaat
     6181 tcattcacca gtttgcaccc agccgtcggt tcctattcat cttggtgcgc gatttgcgga
     6241 tagacactgc tcagccgatg aaaaaacatc aagcaaatat cgaatcaaat aaggcttctt
     6301 cctagaggag aacgtggccc aagaacgcaa gagcggccaa tcggttgatc ccccggaagg
     6361 ccgttacgcg aactacttca agattggcta taacgcgttc gagttaatca tcgattgtgg
     6421 acaatgttac gccgatgatg cagcgccgca actgcactcg agaatcatca ccagcccggc
     6481 ctatggaaaa gcgctgctaa aaacgttgag cgactccctg gaggaatatg aaaaaaacta
     6541 cggccgcgcc ggagaatgaa agtcgctgga atgtgatcgt ctgaagttag gacgactctt
     6601 ttggctcttg ccgtagagtg gatcgataaa taccaggata acgaagatca tcttcaagaa
     6661 ttatcgattt gatagaccgc taaacaggcg tggaaaatac ttggatgaat accgcgatcg
     6721 acttccggat gcaaaggtgc tgatcgtttc atgtacacgg cgctgcgcgc taccagccga
     6781 actctcgcca aatttcttga gaccaggttt caagcggatc cgctgttgag cggatttttc
     6841 ggcggcggaa tggtcgtctc gctcgctacg cctcaggaaa tgaatgaaaa gcccgcggaa
     6901 ggcttgtccg tctggctcta ccggattatt cgcgacgaac aacgcctcaa cgatccgccg
     6961 gttcgcatta gcccgacaga actgcggccg ccgccattgc ccgttcgact gcattacctg
     7021 atgacgccgg tgacgaatga acaaaccggc gatcccgaaa ccgaacaact tattctcggt
     7081 aaggtgctcc aactatttca cagccatccg gtgttggccg gcgcggatct gcaggccgag
     7141 tttaccggga cggaagttga gctgcgtgtt cgcctcgagc ccttgatgct cgaagagatc
     7201 acgcggatct gggaagcgct ggagggttcg tatcagctgt cggtgtctta tgaaatcagc
     7261 gtggtgaata tcatgcctga gttggaaccg gagcaggtca gtccggtcat gatttcgatg
     7321 ccggaacacg gactgatcgt gtcttcctga atgcttgacg tggagttata gatgtcgcgc
     7381 cgcgcgattc aagttgatca cagaatcatt ctctactcac ccgatgatcc ggcgtctgaa
     7441 gtggaccggt ttcaggccgt actgaaagtt cgcgtcacgg atgaattaac cggcgccgcg
     7501 ccgaatagtc aatcggtgct ccaggtcaaa gaacggagat ttttttcacg cttgggaaac
     7561 gatggtctcg gtggacttgt cgccattcca cggcaggttt ttccagcgct ccaggcgcgc
     7621 aactatccgc ttcacctgac tatcggcgcg gcgagatacc agcgtcgcgt cgtccaaaag
     7681 gatatccctc aggacttcaa ttttccgggc gcgtttacgc ctcaggagct tgatatcgcg
     7741 ctgcaccggg cgccggtatt tattgccgga cgcaccgcgc gcttgatcaa caatgccacc
     7801 acgccgctac cggacgcgca gatcaccgtc acgggtatat ggcgaacggc gccgcctgcg
     7861 aatgtctcgg tgcctccgga tccggcgaat atcattgcgc tcgcgccgcc gctctatgcc
     7921 gaccgcgcgc cgctgacgca atcggttgcg gcaagagatt tgacgccagt tagcggcgcc
     7981 gacaaaacgc tgatggacga tgtctccgcc tccgccaatc cgattcgtat ttcggaccga
     8041 tcgggtctcg cagttggaga tattttgatg atcgatgccg acgatccgga tcgggtcgaa
     8101 ttcatcgcga ttaaaaatct gtctacgacg gcgccggcag atcaacccgc ggtcgtctca
     8161 ctggattatc cggtgatttt ctcccatcgg cgaaacacca acgtccgacc cataaaccct
     8221 caaccagcgg gcgtaagtca gacgattacc atagaggcat gggcggggga cgcgtgcgtg
     8281 tttcttaatg gcatcggtgc tctggtcggc gcgcatgaaa ttgagattac cggcgggccc
     8341 gggccggttg agtatcacag ggtgaagacc ttttccgcca cgagcgacac cgagggatat
     8401 taccgtttgc cgccgctcag ccgcgtggcg caactggaaa ttcacgccga gaaaatgatc
     8461 ggcgcgcaga catttcatgc gacgacggtt tttcgtcccg tttatcagca gcgggacaat
     8521 cgccttgatc tcattctggc ggcttgaaat caaagagccg atgatggacg cagtccgcgg
     8581 caacccaacg agccaccaac ccggaggagc agtatgcctg aatatctagc ccctgcagtg
     8641 tatgtcgaag agatcgacag cggcagcaag cccatcgaag gcgtgagcac cagcacggcg
     8701 ggaatgatcg gtctcaccga gcgcggtccg gccaatgtgc cgattcttgt gaccagttac
     8761 ggcgagtacg tgcgctggtt cggcgaccga ctgaatcatg ccgattttgg cgatcactgc
     8821 tacttgcccc atgccgtcga aggttttttt accaatggcg gcaagcgggt ctttatcact
     8881 cgagtggagg caagcggcgc ggcccgcgcg agcttcgagc tgcacgatca aggcgccgcc
     8941 aactccggtt tcaccctgct gctgcgcggc gcccccgtgg ataccggcac tagcgggcat
     9001 acgccgatct atattctcga tatcaataca cctggtgcag gcgcgttagc ggacggcgat
     9061 cgtattcgcg tgggagatga cagcgatgcg gaatatcgga tcatcgataa gattgagccg
     9121 gcggccagca caactcacgt accgctcggt tttccattga gccgcgctca tgcgagcggc
     9181 gttacggtcc atcaaatcaa ccgcgttact agggcggtcc catcgacggc aaccgcgctg
     9241 gcgctggcga acgaagcgca cgccagcgac aatgcggtcg ttgttagcgg cgctgcggcg
     9301 gacatcgcca cgctggctgc cggccaactg ctcgaaatcg gtgatccgtt cttcaacgaa
     9361 catcgattta ttcgggctgc ggtgattgat ccggcggacg acaaaatcgc gagggtgacg
     9421 ctggatgggc cgctggcgct ggaacatgcg gccgcggccg atagggtaag gcggctcgat
     9481 atcgatttga ccaataccgc ggttactgtg gatgacgata cgctgagcgc cgcggcgctc
     9541 ccgaacgatc gggtcgtcta tgtcaccgat cggaacaaca acttcgatga ccgcaccacg
     9601 ttgatcattc tcgacatcaa cagctcggcg acccgggagg cgcggcgtat aggcgcgctg
     9661 tcgatggtga ctctgacaac cggcctctac gccgccattc cggcgggatc cctcatggag
     9721 cgcatcgatc tcggggatga cgcggcggtg accgccaaaa ctctcacggc cgccgcccgc
     9781 gcgggcacgg cggtgattgc cctcgacaat cgcgtcggac tggaggtgaa gcggattctt
     9841 cgcatcggcg ccgcccccga cgatgaatat ctgacaattg cggcattgcc gaaccgatca
     9901 cccggtagtg cggcgccgga tgccggcacc gtggtgcttt cccacccgct caatcgtgat
     9961 catgcgccgg gcagtccggc gcggctgcaa actgatcccg tcgtcaatac ggcgagaccc
    10021 gccacgacat tactgttgga tgccgccctt ggcagcgctt cgcttccggt gaccgacgga
    10081 gacggctacg cgcaggatga gttcgtccgg ctgacgcctg ccggaggtcg gccgctttat
    10141 catcggctgg cggggaatgc cgcgagcctc gatgcgcggc ttgtgaatct gtcggtcccg
    10201 ttatctcgcg cccaccaggc cggagcagcg gtcgcggaac gcgcgccgct gctcagcgtg
    10261 caggcgctcg actccggtga ttggggcaac cgcttgcgcg tttcggtgca agatgaaccg
    10321 gcgggactgg tgccccggac cgctgtcagc agcgttatcg acgccaacca cattcgtctc
    10381 acgtccgcag ccggcgttga agcgggaacg attctcgaag ttttcgatcc cgccggaggc
    10441 gtgacggtcg gcgatctatt gaaagtgacc gaggtcaatc gagctgcgaa cttcacgatc
    10501 gcactcggcg gcgcgggcat ctccgcatca cagtcggcgc cggggctggc ggtgcggtca
    10561 agagaatttc gcctttcggt ctatctattc cggcagccgg atccggcgat accgttgcgc
    10621 aacgagacga ttctcgacag cgaagtgttc cgttatctct cgatggatcc gcgccacagc
    10681 cgctacgtgc aggacatcat cggcgatatc aacggcgccc tcagaatttc cgatcgccga
    10741 cccgaaggcg agtcgagata tatccgcgtt catgacatgg ctcaggattt atcggaaccg
    10801 gatcgaacga cgactctggc gagcatccgc cgcggtccgg aaacgctcgt ggacgtctta
    10861 ccggatggcc gccggcgccc agcgcgccat gcgctgggcg acctggtgcg cggcaccgat
    10921 tcggtcggca cgctcacgga agacgattac gtcggtcagg acaatcagga tccggaggcg
    10981 cgcaccggcc tccatagcct gcgcaacatc gaggagatca gcatcgtcgc ctgcccggga
    11041 cgcacgacgg cggtgatgca gaacgcgctg atcgctcatt gcgagttgat gcgttatcgc
    11101 gttgccgttc tcgatggtcc gcggccgcca caggacacgc ttgccgatgt ccagacgcag
    11161 cgccagcagt acgacacaaa atacgcggcg ctctatcatc cgtggctgct gattcccgat
    11221 ccctatccgc tgaatctgag ccagattgcc gattacccga ttccaccgtc gggccatatc
    11281 gtcggcattt acgcgcgcac ggacatcgaa cgcggcgtgc acaaggcccc ggccaacgaa
    11341 gtcgtgcgcg gcatcatcgg gctgcagcgc attgtcaaca aagagcagca tgacattctc
    11401 aatccctatc cagtcaacat taatgtcatt cgtgatttcc gcgacgcgaa ccgcggcatt
    11461 cgcgtttacg ggggaaggat catcagcggc gatcccgact ggaaatacct caatatgcgg
    11521 cggctgatga ttttcatcga ggcatcgatc gaccgcggcc tccagtgggt ggtcttcgag
    11581 cccaacaagg aagatttatg ggcgcgggtg cgccgatcga tttccaattt tctgactctg
    11641 gtgtggcgca acggcggcct cgaaggaacc aaggttgaag aggcgtactt cgtcaaatgc
    11701 gatcgcacaa cgatgacgca aaccgatatc gacaacggcc ggctgatctg cgtcgtcggc
    11761 gtcgcgctcg tcaagcccgc cgagttcgtg atcgtgcgta tcggcctgtg gacggcgcac
    11821 gcggaagact aaaggtcgaa ggaggaccga ctgatggcta ccggagaacg gcgcgacccg
    11881 tttcgcgcat ttaactttca gcttgaaata gacaacatcg cgcgcggcgc gttcagcgag
    11941 tgcagcggcc ttacagcgga aggagacgcg gtggactacc gcgaaggaac cgacttgcag
    12001 cccaacgtgc gcaagcttgt cggcctgcgc aagtacacca acatcgtact aaagcgcggc
    12061 tatacgcagg aagatacctc gctatgggac tggtacaaaa acatccacaa cggcgtcccc
    12121 gaccggcgca acgtcacgat cattttaatg aatgatgaac gccagccggt gatgcgctgg
    12181 catgccgaga atgcttggat caataagatt gaagggccga gctttaaagc cagcggtaac
    12241 gaagtcgcga tcgagtcggt cgagttggtg catgaagggc tgaccatcga ggtttagctt
    12301 cgaggatttc gatggtgcaa ttaccgcaaa caccaggcgt ttattatgag cgcgtcgacg
    12361 ccagcgctcc cgccattgcg ttgctgcgca cggacattgc cggtttcgtc ggtatcgcca
    12421 cgcgcggtcc cctgcatcag ccgctgcccg tgcagtcgtg gcgccagttt caagcttatt
    12481 tcggtgattt taccggagcg ggctatttgg cttatgccgt ccgggctttt tttgaaaacg
    12541 ggggccaacg ttgctgggtc gtgcgcgtgg cgtcagaaac agcggcaacg gcggcggtaa
    12601 ctctgcagtc tgaaagtcgc gtcggggtct ggacgatttc agcttcgagc cccggcgtgt
    12661 ggggcaatga tctggatctc gtgattggag aaaagcaccg cgcgcagacg ttgaccgtcc
    12721 ccgaaataag cgcgccggaa ttttccacag tgctctcgac caccggattg ggccgcgcca
    12781 ctctggtgcg cctgtcacag ggcgcgacca gaatccacaa agtcgtctcg gacgtcgatc
    12841 ccgtgagcca tcggctgctc tggatcaatc ctaaaagtga agcgcgcctg ccctatgaca
    12901 gcgtattgag cggtttcgat gccggacatc cgatcctgat cgaaagcatc gaatatacgc
    12961 tgtcggtgag ggagcgcggc cggctgattc gtgtctacga aggactttcg ctcgttcccg
    13021 agcacctgcg ttatggtccg gctctgctgg gacaatttaa attgccgcgt ttctccggtc
    13081 agccggtaaa tctgccggcc gcacccgagc cggtaacgat aaccgaattg cgcgcgttgc
    13141 cggacgccga tctcgagtcc attgccacaa tcgcggatca gattatgccg ttgactggcg
    13201 gcgcagacgg cctggctcag ttgagggcct atgatttcat cggcgaggat ctcgatccgc
    13261 tcgacagcga tgaggtccgc atacgtaaag cgcgcggcct gcgaaccctc gaagacgtgt
    13321 gcgagatagc gattgtcgcc gtgccggata ttcacattca accgaaagcc gcgccgcgtt
    13381 tcagcccgcc gccgccatgt gttccggatc cttgtctgcc ggtgaacctg ccgccggcgg
    13441 tgccgcgcac gccgtcgctg ggcgacctac cgccggtctt tccggatgag gccatctatc
    13501 aggtacagtc cgcattgatt caacactgcg agaagctaag cgatcgcatc gcgctcctgg
    13561 atccgccgtt tagcgcgtcg cgcgatgaca aactcggtat cggtgcggtt cgcgcttggc
    13621 gcagccgctt cgattccaag tatgcggctt tctattaccc gtggctgcgc gtggtcgatc
    13681 ccttgcgctc gcccatgtcc ctggtgcgcg agattccgcc caccggccac gtggccgggc
    13741 aatacgcgca aaacgattct caattcggcg tgcacaaggc gcccgccaat gcgccgctca
    13801 tctgggccga ggatgtgacc gtgttcgttg atgatgccgt gcacgggatt ttgaatggca
    13861 tcggcatcaa tgcgatccgg ccattgccgg gccgcggcct gcgcatctcc ggcgctcgca
    13921 cggtgagcag cgaccccgat tggcgatacg ttaacgtgcg ccggctgatg atgatgatcg
    13981 aaaaagcgat cgagacggcg atccagtggg cggttttcga gccgaactcc actctgacgc
    14041 ggaccaagct caatctgacg ttaacgagtt ttctgctttc cttgtggcag cggggagccc
    14101 tcatgggagc atccgcggag gatgcgtttt ttgtcagatg cgacgacgag aacaatccgc
    14161 tcgaagagcg tgaccacggc cggctcctga ctacaatcgg cgtcgcgcct tcgcggccgt
    14221 tcgaattcat tattctgaga gtcggacgcg cgcacaatca gttcgaaatc agcgaagcgt
    14281 cgtccggtgc ttccgggctg gaggtcttct agtggcattg aatcctacgg gaaggcgcga
    14341 cgatcctgtg ttgaatcata acttcgtcgt cagtctgctg gacagcagca gcgcgagcag
    14401 tctgttcggc tccgtttcca tgtcagccat tttcgatgtt gccctgggcg gcttcagcga
    14461 gtgcggcgga ttggagatgt cgcttcaggt tcaagagttc caggagggcg gcaacaacgg
    14521 caggttgctc aagtttccga cgcgggtgac ctggtcgaat atcacgctca agaagggcgt
    14581 gagctcgggc acggcgctgt gggactggca ttacgccttt acggccggga ggggcagccg
    14641 tcgcgacggc gtgattgtgt tgctcaatga ccggcagttg ccgaacaaca tctggtattt
    14701 ccgccgcggt ttgccggtca agtataccgg gccggcgctc aacgcgactc agagcggcgt
    14761 cgccattgaa tccatagaaa ttgcccatga gggactttcg caggtgccga ccggtttggc
    14821 gccatagctt gtcaggagga ttggaatgga agttcagatt cagacattgc aaagcacggt
    14881 gcaggcgttc gacggggatg tggcgctatc gccgcagact atggacaaga tcgtccgcgc
    14941 ggtgcttcag gcggtgaatg atgagcaggc gcatcgtcag cgcgtccgcg ccgagcaacg
    15001 cattacgact ggggtaagtc acgagctcga agagagggat taaaatcgtg gaacaggtcg
    15061 cgaaagcagt cttgcgcgtt gaatgggatt ttccacggaa tccggaaatg atcgaggttc
    15121 aatataatcc gaccgagctg tcatgggaca aaggcgccca gatcgccgag attacgattc
    15181 caggattgga tgcgccgctg caacaatttg tccgcggcca agccgagaag ctgaacctcg
    15241 agctattttt cgacagcacc gatcacggca tgggcaaggg agcggtgagc gtcacgacgc
    15301 tgaccgaccg aatctatcag ctgatcaaga ttgagccgag ccgtcatgcg ccgccggtct
    15361 gtacttttat ctggaacgat gcatttcccg gcagctcact tgagggaaat gccgggagcg
    15421 ccgcaagcct tgcgatcggc ggcagtttcg gcgtggcggc gggcgtggtg tcttcggcag
    15481 cgggcgtcgc cgtcagcgcg gcagccagcg ccgtggctgg aatcgccagg gccgcgagcg
    15541 gcaatcagcg ccgcaacggc ttcaggtgca tcgtcgaaag cgtcaagcaa aaattcacac
    15601 tttttagctc ggaaggtgtt ccgctgcgcg cggcattgag cgtggcgttg cgcgaataca
    15661 agactctcaa tgaccaactt gctcagttga atttgacctc gcccgatcgc acgcatagtc
    15721 acgtgaccca aaacggcgat tcgctgtcac ggatcgccgc gcagtactat cgaagcgccg
    15781 gcgattggcg gttgattgcc gaggccaacg gcgttgaaga cccgcgccgc ctcgtggtcg
    15841 gcgctctttt gcagattccg ccgacgcaat aggagatttg gcatgccggt agtgacgctc
    15901 ggggatcaat cgaaactgca gggcaatttc tatgtcccgc gcttcgaagt gaaaatcgcc
    15961 ggtgccaacc tgccgcagaa cgttttgcgt gatgtcttgc aacttaccta ttcggataac
    16021 gtcaaggagc tggatagctt tgagtttacc gtcaacaatt gggacgccga aaagcacgaa
    16081 ttcaagtacg tcggttcgga aacaaccgat tccctgagca gtaatccgct gcaccggctt
    16141 ttcaatcctt cgcgccatga ggtcgaggtc tcgatgggct atgagaataa tctgacgctg
    16201 atgatgaaag gtagtttcac cacgatggag cctacctttc ctaacagcgg cggccccaca
    16261 ctcaccgtgc gtggtctaaa tatcctgcac aagctgcgca gcgggcagta cagctatgca
    16321 tggatgcctg atgaaaaaca tccaaatgga tggaaggaga gcgagatcgc taagagtctg
    16381 aggaaagtgc ccgatctcga ccacaaagaa gaaggcaaga acaaggaggc caagaaacca
    16441 ttcccgctgg aggtcgaaat cgacgaaaac gcactcagca aagagcatcc gatcacgtat
    16501 gtggcgcaag acggccagta cgatatcgat tttcttctct cccgggcgcg gcgcatcggc
    16561 tatgtcgtct tcgttaaaga agaggagcgc gaaaagggcc gggtcaccaa ggagaggcgg
    16621 ctctatttcg gtccttccga agccaatcat cccggcctgc gcccgctaac atttgaactc
    16681 aaatggggcg tctcgctgat tgacttcaag ccgacattaa cgaccgctaa ccaagtcaag
    16741 acagtggtcg ttcagggaac cgatcgatcg acgaagaaac caatcaccgg gaaagcatcg
    16801 ctcgatgagc cgagattcgg attaaaccgt gacctgatca aactgttgga agcctccgac
    16861 gctcgcgatg aacgtgtggt cgataggccc gttgcgaccc agcaagaggc tgatgagttg
    16921 gcccgcgcga tcttgcgcga gcgcagcaag gatatcctca aggcaaacgg tacttgtgtt
    16981 ggcctgccgg agcttcgcgc cggccagcgg ataaagatcg gcgggctcgg cgcgcgcttc
    17041 agcggcgaat atttcgtcac cgataccgtg catacgatca acgacagcgg atacatcaca
    17101 agatttggcg cccgccgcga acatcaagga aaatgagctt gatccaatga agcattatcc
    17161 gggagtagtt accgcgattg tcaaaaactt gacggatcct aacggccagg gtcgcattga
    17221 gttgcaattc ccctggctct cagactcgtt gcgaagttat tgggcgccgg tggcggcgcc
    17281 tctcgccggc aaagatcgcg gcatgttctt catgcccgaa gttgacgacg aagtgttggt
    17341 cgcgtttgag catggaagtt tcgatcatcc ctacatcgtt ggttatctct ggaacggcgt
    17401 ggacatgccg ccggagaccg acctgaaaaa tcgtgtcatc aaaacacctg gcggacatca
    17461 attgcgcttt gaagatgcag atggcgcaaa aaaagtgatc ctgaaatcaa atggcgggca
    17521 tcaagtcgag atcgacgacg ccgcagagac catcacgatc aaaacgaatt ccggcaatca
    17581 gctcatcgtc ttaaacgacg ccgccaaatc cgtaacggtg agaggtggtg ggcggagagt
    17641 ggatatggtc ggcggtcaaa tcaaaatgac ttagtaggga aagtagatca atgcctcagg
    17701 ttgtaacgac caatacccag atcctttgcg ctcacttttc gacggcgcac aaaaacccgc
    17761 cgctggcgcc attatggacc atcgatggcg gcactgttct tgtcgaaggt gattcgggaa
    17821 cgtttccgag ctgtccaagc accttccagt gcggcggata ccaactgcgc tctatgggct
    17881 tgaatgccac tcaagttacg gggaagaaag tgattctcgt caccgatttt aatcagacat
    17941 cgtcaggctt gccaataact atgattgaaa cgcatccggt gtttgacgag acaacaccag
    18001 cggccattcc cgacggtcag ccggcccctc cgttgccgcc cgaaatgacg gatcacgtta
    18061 agccagtagt cataagctca ttacccgctc tcgctttcaa taagacttcg atgacgccgc
    18121 cctcggttct cgtgacgttt acgctcactt ccgcgcatcc gatgcgctgg atactcaccc
    18181 aaatcgacga acccggcccc caacccggca cccatgaccg aaccaatgcc ccgcagcccc
    18241 caggaatgac agtcaatcct tcaggtggcg tttggaatat ttctccactg aatatcaacc
    18301 tgacgatgac ggcacccttc atggctagtt tactgccggg ggatcaccga ttttttctga
    18361 cgggggtgac ccagcgcgga ctatcgaact tcgccgaagt attactaacc gtagcagtct
    18421 aaagccgaga aagcggaatg aacggaacgc aacgaagcag tcaacgagaa ttcctcggtg
    18481 tcggctggaa atttccgctc caggtcacac cgggtggcca gatcgcccaa gcgcgctacg
    18541 agcagcgcat cgaggaatcg atctatctca ttctcagcac gtccaagggc gagcgtgtga
    18601 tgctgccgga atttggctgc ggcatacatg atctggtttt cgctccgaac aactcgacga
    18661 cgatcgccgt tgtggtccag acggtgcgcg aagcgctggt cgctttcgag ccgcgcatcg
    18721 atgtgctgga catcagcgcg gagagcgcgc cggaagagcc caatcttctg ctgatccgaa
    18781 tcaactaccg gattcgcgcc aacaacgcgc gcggcaatct ggtttatcca ttttatattc
    18841 aggaaagcgc ctgatttatg cctctggaaa atgcccttcc ccgcatcgat gaccgcaact
    18901 acgacagcct gatcgccgaa gtacgcacac gtatcgcgcg ctacactccg gaatggactc
    18961 cggtctggac cgatgtcaac gacaatgatc caggcatcac tctggcgcag gtcttcgcat
    19021 ggctgaccga gatgctcgcg taccggatga atctcgtgcc cgagctcaac tacatcaagt
    19081 ttctccagct catcggcatc gagcttcatg ccgccgaacc tgctcaggcc gaggttacgt
    19141 ttccagtgca aagcggattc gccgaggcat acgtgatcgt gcccccgcgc acgcagctta
    19201 ccgcggatgc gccggatggc agtgcgccga tcactttcga aaccgatcgc gcgttgatcg
    19261 ctgtgcgcgc tcagctcacc gcggtgctgg cctatgacgg ctatgcgtac acgtcggtga
    19321 ccgccgacaa tcaggaggcg gtgcagggct ttcaaccttt cggcccgatg gccaacgacg
    19381 gcagcgcgtt gctgctcggg tttgacgatc ggggtccgtt cccggccctc gagctgaacc
    19441 tggcgatcgt gaccccggac aattccgcgg cggccgcgtt tcaatgcggc ctgcccgacc
    19501 ggcccggctt cgcttccgcg acgctgaggt gggaattctg gaacggcagc gactggcaac
    19561 gattgagcct gctcaaggat gaaacccgag cgttcacacg ttcaggtcat gttttcctga
    19621 aaaccccgaa caagagcctg cagccggttg tcatcgcgcc tgaatcccga ccgctttact
    19681 ggatccgcgc gcgtgtcgaa cgcagtcaat acgagcgtgt gcccaagctt ctgacgattc
    19741 gcaccaatac gattgccgtg cgccaggccg agacggtgcg cgacgaagtg ctcggcggca
    19801 gcaacggccg gcgcgatcag gtatttcggc ttgcgaacgt tccggtgctg cgcgaaaccc
    19861 tgcggcttga aatcgacgaa ggctcgggtc ccgagatctg gactcgggtg gacgatttct
    19921 tcggttccgg gccggcggat ccgcactaca tgttgaatcg gaccactgga gaaattcgct
    19981 tcggcgacgg gcgcaacggc aacattccag tggctaaccc ggcaaatccc gaaggcaaca
    20041 tcgtcgcccg agagtatcgc ttcggtggcg gcaggcaggg caatgtcggc gccggagcga
    20101 tcaagacgct gaccacatcc atcgacggca tcgattccaa cgcggtggcc aatctgcttg
    20161 cggcggacag cggccgcgac gaagaaactc tggacgaagc aaaaaaacgc gcgccccacg
    20221 cgatccgcag ccgctatcgc gccgtgaccg cggaagattt cgaattgctg gcgatggagg
    20281 cgagctcgat caaacgcgcc aaggcgttgc cgctttacca tccggatttt cccggcgtca
    20341 aagttccggg cgtggtgacg atcatcgtcg tgccggacgg tgacggccca tcgccgacgc
    20401 cgagcgaagg caccctgcgc agcgtgtgcg cctatctcga tcagcggcgc ttgctgacaa
    20461 ccgaactgta cgtgatcaag ccgacttacc agcgggtgca gatccgtgcc gagatcgtcg
    20521 tcagcgacga cgcagatctt ggcgaagttc agcaacagat ggaacagtcg ctgctcgact
    20581 actttcatcc gttgaaggga ggcgaagacg gcttgggctg gcctttcggc ggcactattt
    20641 attattcacg gatcaatcag cgctgcttca cgatcccggg agtcgccagc ataaccagtc
    20701 tcataatcgt cctcgatggc gaggaaattc cgccgtgcac ggatgtgacc atcaacgaag
    20761 ccggactgct gttcgccacc gaacatcaga tcgacgtgag ttaccgtttc gagtcctgaa
    20821 atcatgatga ctgccagtca acttccgccg attccgcggc cgccgcacga tcccacctcg
    20881 ctgctcctca actggcggac tggctggcgc gctgccgaat tgcaacagct cagcgcggag
    20941 cgatcgctgt taatggcgcc tgcgccggaa acacagcgtt ccctgacgga aattagcggc
    21001 agtttcggcg ggctcatggc cccggccaat gtcgcttgcg ggcccgacag cggcgtttat
    21061 ctcctcgatg ccgaaactgc gcagttgaaa cgtttcgatc cctgcgcatg ccgctttacg
    21121 caggtgcctt gtttcggggg tacgggaagc gggccgcgac agctgaaaag tccccatggc
    21181 atcgccatat gcgccggcaa tctgtttgtc tgcgacaccg gtaatcatcg cctgagcgtc
    21241 ttcgccttgc atggatttgt gctgcgcggt ttctggcagc cgccgcccgc ggcttataaa
    21301 gggtcgcatc ccactttggc caatttatgg gaacccttcg atgtgacttt cgacggcgtt
    21361 ggccgagctt acgttaccga cggcgccaac ggttgcattc atcgtttcac tgccgcgggt
    21421 cagtgggaaa actgcttcac cggtttcggg caggcgacct ggatagccat cgattgccgc
    21481 gaccggagct acgtgatcgt cgctggctcg ccggcgcagg tcgtgcggtc ggatccggac
    21541 ggcaaccgta tcgcgatcga ttccatgccg ggcgagctga tgtcgctgtt tccgcggaat
    21601 tcgatttcag tcgatgccgc ggggctgctc catctgggcg ccttttgcgc cggcgacgcg
    21661 ccgatgaatt gccaaaaccc gcagcctacg ctcaagtgcc cgcggggtca aacaccggag
    21721 cgcggcgtct tcgacttacg aggcaacacc gtcgattgct tcgtctccac acccgcgagc
    21781 tatgtgacgc aagcgaccta tatcagcgcc gccctcgaca gcgagttcta ccgctgccag
    21841 tggcatcgga tcattttgcg cggcgagatt cccgccgggt ctcgagtcac ggtttcgacg
    21901 tacacggcgg aagccgagct ggcggacgat gacatcgaga atctcggcga ggtctggcag
    21961 accaatcaga cggcgaccga aatgaacaaa ggcgactggg attgcctggt gcgcagtagt
    22021 ggcggccggt ttctctggct gcggctcgag ttccgcggca acggccgggt gacgccgcgg
    22081 ctcgacagcg tcgaactcga gtttccgcgg atcagcttgc gtcgctacct gcccggcgtg
    22141 ttcggcaggg aggccgtcag cgccgacttc acggatcgtt ttctgagcct cttcgataca
    22201 acctttcggt ccattgaagc caacctcgat cgcggagccc gctatttcga tccgctttcg
    22261 gcgcccgctg aacgcgatcc caaaaccggc gcggattttc tctcctggct gggttcatgg
    22321 atcggcctca ttctcgatcg ccattggccg gaggccaggc gcaggaaatt ccttaagcac
    22381 gcgggccggc tctacgatct gcgcggcacg cgcgaaggtt tgtggcgcga gttgttgctg
    22441 tttctcgatc tggatcgcga cgagtgttgc cgggctgatc agccgtccga ccgctgccag
    22501 gcgcggcctg cgaactgcgc cccgccgaag ccgtctgaat gcggctggcg gccgccgccg
    22561 ctgatcctgg agcacttcaa gttgcgccgt tggctctttc tcggcgctgg gcgtctggga
    22621 gatcaggcgg tgctgtgggg aaaacgcatc gtcaaccgca ctcagctcga cgataccgca
    22681 cgggtggagc gcacgcagtt gctaacggcg caggatccgc tgcgcgaccc attccatgtg
    22741 tatgcttata aattcagcgt gtttgttccg gcatgttttc gcgcgtcgaa taccgaggcc
    22801 aaggcgctgg aaaatattct gcgcaacggc aggccggcgg ggacccaggc gcagctcgaa
    22861 tacgtcgagc cccgttttcg tatcgggttt caatcgatga tcggttttga cagcgtcgtc
    22921 ggccgctatc cgtcaggggt aactttaaac gagatgcagc tcggccgcgc cacagtcttg
    22981 acggcgccgc cgcacaagca gggcgctccg tcgctaacgg tcggggaaca gtcgcgcatc
    23041 ggaaccacca cgcggctgga ataaggacga tccacgagat cactgcgcga ttccaccagg
    23101 agaacgatca tgactcatgc acaaaacgtt gttgctcagc ttgcctgccc ggcctgcaat
    23161 tacgggccgt ttacgcgcaa caactacttc accggcaagc tgctggtcga gcgggatttc
    23221 accgatgaga gccgttttca catagagaag ctgcgccacc acgagcagct gctgcacggc
    23281 tggggagtgg tctgcggtct caaagtcaaa gcacacccca acgaagcctg ccgcgatcgt
    23341 tttgtctgcg tggagcccgg ctcagcagtc gattgctgcg gccacgatat tatcgtcgcc
    23401 gaagaagagt gcgtcgattt tagtcagttc cccgaaatca aggcgctcaa gagcaacaac
    23461 gacaaggatc cgcacacgtt gcagatctgc gcgcggttcc gtgaatgtcc gaccgaagac
    23521 attccggtgc tgtacgatga ttgcggctgt gacgatacca agtgcgcgcc caatcgcatt
    23581 ctcgagtctt atgagatcga catcatgctc gatccaccca aacagccgca agatctgcat
    23641 acgccggaat tcagctggga gaactccatc gctttagcgc acgcggccgc agctgtggtg
    23701 cacgatgcgt cgcatcgttt atatgttctg accgcggatg atcccgccac cgtctatcag
    23761 attagcaccg acaatcacgc gacgatcact gcgcggacat tgaccaagaa ggccatcgcg
    23821 ctgagcgtat ccaatgacgg caagcatctc tatgtggtgg tcgagccgac ctcgccggcg
    23881 accttacgtc ggctgcaggt gctggatacc acgcagcccg gaatgccgga tttcaatacc
    23941 gatccgctcg atctgccgaa cagcttcggc agcgatgtga ccctggccgt cacgccagat
    24001 ggccggctga tctcgctggt ctcgtcgagc ggcgatgtgc ttcgctggcc cgccgatctc
    24061 gacacgaacc cggttcccgc cgcgccggag ctggtcaaaa atctcggcac cggcgtgacc
    24121 gggctgacgc tgagcagcga cggcaagaag gcttttgtgc ttggatctgg aaataagatc
    24181 caagcgctgg atctcggcgc tgcaacggtc actccgatca ccgtgctgcc tgccgccgcg
    24241 aagccttcgt cgatggccct ggtcacgagc accgcgccgg acatgcttgc tgtcgccgat
    24301 cagaccaatc tctttctgca cctggtggag ctgctgccgg ccgccgcatt aatcggcagc
    24361 gtcaagctcg atcacccgcc ggtcggcctg gttgcagcgc cgggcggaca ctgggcctac
    24421 gtgctcgaaa gagacaccga cagttttatc cagtccgtga gtctcgacgg tctcgtgcag
    24481 catctagggg tgacactcgg cacagcgttc atgatcggcg acgacagccg gcagatcgtc
    24541 atgagcgcat cgggcaagcg tctctacatt ccttacgtcg atgatattag ccagaccgcc
    24601 gccggcggtg tcgcgattgt ggaagtcacc gaaggctcat gcgcggagat tctttggcgg
    24661 catctcgacg gctgcccgca ttgcgacctg cccgattgcg tggtcctggc gaccatcgag
    24721 aattatcatc tcgaagatcg cgtagaagaa caaaccgatc cgccggccga tgcggcacaa
    24781 gatgccataa acaaaattgc gcggatcgac aaccgcaaag gaaggcgatt gctgccgagc
    24841 acgcaagttc tgactgagct ggtggaatgt ctcctcgaac atggtgtggg aggagcagga
    24901 acgcaggggc ctcccggcgc gcccggcgcg aaaggcgaaa agggagataa aggtggcaag
    24961 ggcgataagg gcgaaaaagg agatcaaggt ttaaaaggtg acaagggaga ttcgggttca
    25021 ggcctggaat taggtctggt tcgaatcaag gcgttgagtt ggactcacaa cacgcccagc
    25081 caccttatcg gagtgaagat gctcaaccag acacaggtgc cgggattcgt gatcggcttt
    25141 accgacaagg tgcacgtaag aacccaggat cagtcagggg cactcgtcgg cctcatcgac
    25201 gcctcgcata tctttcaggt tctcgtggcg tccgatcttg agcagaacaa agaactcgga
    25261 atgttttgtc gctgccccat cgccggcgac attgttccag tcgattacac cgaaacaaac
    25321 ggccttatca ccactgccac tgaagtgcag gacttagatg cacctggcat cgcctttatt
    25381 cttgatcgca gtaggtcacg gcgggctggt gaaatcattt cgcaaacccc tccgttcgac
    25441 aagcctccgt tcaacgacat ttggatccgg ctgcgcggcg attttgtgct ggatacgaaa
    25501 gatagggcaa ttgacgccga atttgtgcgg gcaaaattac ctactggaga ccgtccggac
    25561 ccaggaggat tgcctttgga caaacaaccc gggattcaag gtggagtctt cgaaagttgg
    25621 ttcacgctcc taccgggata gaaagtttca agcggttcag atcgagtaag gagaatcttt
    25681 atggcactcg ctacaacaac cactcgaacg cctacagtcg cacgctgccc ggcttgcggc
    25741 aggctcgaat gcttgtgccg gccgcgattt ttcgccggcc aactgttgac cgaagaggac
    25801 ctcaaccgtc tcgatcatta catcgtcgag aaaaacaaat tgcataatcg ctatctgcac
    25861 ggttggggcg ttgtctgtgg cctggaggtt ttttgccatc cctgcgaagg gcaggtcacc
    25921 gtcacctcag gatacgcgct cagcccctgc ggtgacgaca tcgtcttgtg cgccaacgat
    25981 gtcgtcaacg tctgcgatct gatccagcgc tgccgcgagc gcgagcgcct ggattgcgag
    26041 ccgccgcgtc ccgccacaac cgcggagtgc caggacgtcc aggaacaatg ggtgctgacg
    26101 gtttgcttca ccgaaaagtc cgcgcgcgga gtgacggcgc tacgcggcag cagtgaatcc
    26161 gcatgctgct cgagttgcag ttgcggcggc tcctccagct gcggctgtga ggaaagcggc
    26221 atgagtcgga agcaagcggc gccggccacc cgctccgccc gctcgctgtc caccgatgcg
    26281 cggtccggcc gcgacctgtg cgagcccacg gtcacttgcg agagccacta tttttcactc
    26341 tacaaagcat cagctgccga tcctcgaccc gaacaggctc tc
//
LOCUS       DASUVS010000381         5337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1695356, whole genome shotgun sequence.
ACCESSION   DASUVS010000381 DASUVS010000000
VERSION     DASUVS010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695356"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VF182_20360"
     CDS             complement(<1..210)
                     /locus_tag="VF182_20360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439480.1"
                     /translation="MALVGLGLMFAPQRFGVGAAATDASAALVAYLRLFGSPFLGIAV
                     LNWMARNAEPSTARNAIILGNMVGFA"
     gene            complement(389..787)
                     /locus_tag="VF182_20365"
     CDS             complement(389..787)
                     /locus_tag="VF182_20365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HEX6439481.1"
                     /translation="MPEPRSVEPQAARRAAEAVPGIPRDDNGPVFRAPWEAHAFAMAL
                     ALYEKGLFAWPEWSAMLGEEIKKAQAVGDPDTGETYYHHWLATLERMVAQKGAASAQA
                     LAQHYQAWERATQRTPHGKPIELKPEDFGK"
     gene            complement(780..1439)
                     /gene="nthB"
                     /locus_tag="VF182_20370"
     CDS             complement(780..1439)
                     /gene="nthB"
                     /locus_tag="VF182_20370"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HEX6439482.1"
                     /translation="MDGVHDMGGMDGFGKVEPERNEPVFHHPWEGRVLAMSRAIGVFR
                     AWTIDTSRYAIELMAPSFYLTRSYYEKWFLRNQNLLIERGLIDADEVAAGRALRPAKN
                     LTRGPFTLADVDKVTKRGSYSRPAPAPARFEIGDRVRTKNIHPKTHTRLPRYARGRVG
                     VVERLHGAHIFPDSIAAGKGEDPQWLYTVRFDAQELWGADTDPKLKVSIEAFEPYLEH
                     A"
     gene            complement(1439..2065)
                     /gene="nthA"
                     /locus_tag="VF182_20375"
     CDS             complement(1439..2065)
                     /gene="nthA"
                     /locus_tag="VF182_20375"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEX6439483.1"
                     /translation="MAEHDHDHDESSHLSEVELRVRALETILVEKGYIESAALDRIVE
                     LYETKIGPHIGAQVIAKAWNDSRFRQKLLEDASKAVAMLAPRVGNVGEHLIAVENTPQ
                     VHNMIVCTLCSCYPTDVLGLPPTWYKSFAYRSRAVKEPRGVLADFGVALPIETEIRVW
                     DSTAETRYLVVPMRPAGSEGWSESELAKLVTRDSMIGAGVAKKPSEVA"
     gene            complement(2235..3305)
                     /locus_tag="VF182_20380"
     CDS             complement(2235..3305)
                     /locus_tag="VF182_20380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439484.1"
                     /translation="MKLDNRVGFRALAPAWVIAMFLCIPAGQAFSQADFYKGKTIRIL
                     RGGGPGGSGEFQTRALMRVLEKHIPGHPNLLLVFVSGAAGRKAANMIYSSTPPDGLTI
                     GSIGAGLVVGPILGLPGSQYDLDKFIYLGSTDSGDPYVFYTKADAGWDNLAKLQAASG
                     IRFGAHAVGHPVYVTGRMVAYLLGLKEPKFVTGFTGPEIYIALERKELDAHATGAARF
                     VIEKPEYIEKKLVHLHATLTVPKGRHHPKFANLPEFGSFAKSDRERKLLDMFRAFQYP
                     RWPFIFPPGTPQEPVRIMREAMRKSFADPEFRVEFTKLMGDPPAPLTGEEVEQAIKEL
                     PRDKEVVELYKQMAGGGPLPAR"
     gene            complement(3366..3605)
                     /locus_tag="VF182_20385"
     CDS             complement(3366..3605)
                     /locus_tag="VF182_20385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439485.1"
                     /translation="MRLQHMKGGDGRGENDWMIAIYRTSSIVARVCQCAALVLASSQR
                     AINNPSCEYRHPLVMAKLERGNNFLTHFPSDLRRL"
     gene            3676..4482
                     /locus_tag="VF182_20390"
     CDS             3676..4482
                     /locus_tag="VF182_20390"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6439486.1"
                     /translation="MINVEGEPIEVIDAHSHMGARKKLAIHQIPPIMKFMAEDMLESM
                     DGAGVDGVVTFAIGIGEPSDYRETNQYIADAMKKHPGRIHGFMRLSPGNGPKDTLQVL
                     EEGVKLGLKGIKIHPLIEHCPANDKEKVYPLMEAAQHHGLTVLFHCGLGEDASPKRIG
                     EVAKDFPKLPVIMGHSGLVEGVREVVEIAKRCENVHMDSSGVGWLPFFCESISWAGPD
                     RVLYGSDHPFNPMDWEIEKIVKHAQRHLKLKIEDLRLIMAGNIKRLLKMN"
     gene            4490..4801
                     /locus_tag="VF182_20395"
     CDS             4490..4801
                     /locus_tag="VF182_20395"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX6439487.1"
                     /translation="MKIAALADVKAHLSAYVDECENEGPVIITRNGKAAAILLAPKND
                     DDLERLMLAHSRRLQSLLSKSRRSIKAGKGVSHDEFWKTVDRRHRGKRPSRARKRKSV
                     K"
BASE COUNT         1201 a         1527 c         1517 g         1092 t
ORIGIN      
        1 agcaaatcca accatgttgc cgaggatgat ggcgttccgc gccgtcgacg gctccgcgtt
       61 gcgcgccatc cagttaagaa ccgcaatgcc taggaacggg ctgccaaaaa gccgcagata
      121 ggcaaccagc gccgcggagg catcggtggc ggcggcaccc acgccgaagc gctgtggggc
      181 aaacatcaat cccaggccga cgagagccat gtagatcgcg acgatgctga gtagaaccct
      241 gagtttcaga aaagcttcct ttcggtaatt gatctccctg aatcacgcgt agacacggga
      301 ggggttccgg caactgatgg agatgagaga tagggacgtc gtttggccgg tggagaagca
      361 ccgcttccaa agatctaacc gccgagcctc acttaccaaa gtcttccggc tttagctcga
      421 tcggtttgcc atgcggcgtt ctttgagttg cgcgctccca ggcctgataa tgctgcgcca
      481 gagcttgcgc cgaggccgcg cctttttgag cgaccatgcg ttcaagtgtc gcaagccaat
      541 gatggtagta ggtttcgccc gtgtcgggat cgccgaccgc ctgcgccttt ttgatctcct
      601 caccaagcat cgccgaccac tcgggccagg cgaacaggcc tttttcgtac aacgcgagcg
      661 ccatcgcgaa cgcatgggct tcccacggcg cgcgaaagac cgggccgttg tcgtcgcgcg
      721 gtatgcctgg aacagcttcg gcggcgcgcc gggcggcttg cggttcgacg gaacgcggtt
      781 caggcatgtt ccagataagg ttcaaacgct tcgatggaca ctttcaattt cgggtccgtg
      841 tctgcgcccc aaagctcctg ggcatcgaac cgcacggtgt aaagccattg cggatcctcg
      901 cccttgccgg cggctatgga gtctggaaaa atgtgcgcgc catgaagccg ctccaccacg
      961 ccaacacggc cgcgcgcgta gcgcggcaaa cgcgtgtgcg tctttggatg aatgtttttg
     1021 gtgcgcacgc ggtcgccgat ttcgaaccgc gcgggcgccg gcgccggccg gctgtacgaa
     1081 ccacgtttgg tgactttgtc cacatcggcc agcgtgaacg ggccgcgggt taaatttttc
     1141 gccgggcgca gcgctcgccc ggcggcgacc tcgtcggcgt cgatcaggcc gcgctcgatc
     1201 agcagatttt gattgcgcaa aaaccatttc tcataatagg agcgggtcaa atagaacgac
     1261 ggcgccatca attcaatggc ataacggctg gtatcgatcg tccaggcgcg gaatacgccg
     1321 atggcgcggc tcatcgccag cacgcgtccc tcccaaggat gatgaaagac cggctcgttc
     1381 cgctcgggct cgactttgcc gaacccgtcc ataccaccca tatcgtgcac gccgtccatc
     1441 aggccacctc gcttggtttt ttcgccactc cggctccgat catcgaatcg cgcgtgacca
     1501 acttggcgag ttcggattcg ctccagccct ccgagcctgc cggccgcatc ggcacgacca
     1561 ggtagcgggt ctcggcagtc gaatcccaaa ctcgtatctc ggtttcgatc ggcagcgcaa
     1621 ccccgaaatc cgccaggacc ccgcgcggct ccttgacggc gcgcgagcgg taggcaaacg
     1681 acttgtacca ggtcggcggc aggccgagca catcggtcgg atagcaggaa cacaacgtgc
     1741 agacgatcat gttgtgcacc tgcggcgtat tctcgacggc gatcagatgt tcgcccacgt
     1801 tgcccacccg tggcgcaagc atagcaacgg ccttgcttgc atcctctagc agcttctggc
     1861 ggaaccggga atcgttccac gctttggcaa ttacctgggc gccgatatga gggccgatct
     1921 tggtttcata aagctcgacg atgcgatcga gcgcggcgct ctcgatatac cccttctcga
     1981 cgaggatggt ttcgagagcg cgcacccgca gctccacctc ggagagatgg gaactctcat
     2041 cgtgatcgtg atcatgttca gccataggtg aaatataccc aaggaaggcg gcgtcgtgca
     2101 atcggcaaaa cgataatcgg gtgcgttcgt atcagccgtt tagttctatt tcggcagagg
     2161 atcggctaac gtattccctt ctgcaagcgc gggccggttg acccatcgtt agaggacgtg
     2221 caaccgaaaa acaatcagcg agcgggcagc ggcccgccgc cggccatctg cttgtagagc
     2281 tcaacgacct ccttgtcccg tggcaattct ttgatcgcct gctccacttc ttcgccggtg
     2341 agaggcgcgg gcgggtcgcc cattagctta gtgaattcga cgcgaaattc cggatcggca
     2401 aatgacttgc gcatcgcctc gcgcatgatt cgcaccggct cttggggcgt gcccggaggg
     2461 aagatgaaag gccagcgcgg gtactgaaag gcgcggaaca tatcgagcag ctttcgttct
     2521 cgatccgatt tggcgaagct accgaattcc ggcagatttg caaattttgg gtgatggcga
     2581 cctttgggaa cggtcaaggt cgcatggagg tggaccagct tcttctcaat atattcgggt
     2641 ttctctatga caaagcgtgc cgcgccggta gcgtgagcgt ccagctcctt ccgctcgaga
     2701 gcaatgtaaa tctcgggacc ggtaaagccg gtgacgaact tcggctcctt aaggccgagc
     2761 agatacgcca ccatgcgtcc ggtgacatag accggatgac cgacggcgtg ggcgccgaag
     2821 cggataccgc tggccgcttg cagcttcgcc agattatccc agccggcatc agccttggta
     2881 tagaagacgt aaggatcgcc ggaatcggtc gaacccaggt aaataaattt atccagatcg
     2941 tattggctgc ctgggaggcc caagatcggc ccgaccacga gtcccgcgcc gatagagccg
     3001 atggtcaacc cgtccggcgg tgttgaacta taaatcatgt tggcggcttt tctaccggcc
     3061 gcgccggaga cgaaaacaag cagtaaatta ggatgaccgg gaatgtgctt ttccagcacg
     3121 cgcatcagcg ctctcgtctg gaactccccc gaaccgccgg gcccgccgcc gcggaggatt
     3181 ctgatggttt tgcctttgta gaagtcggcc tgtgaaaagg cctggcccgc agggatgcat
     3241 aaaaacattg cgatcaccca tgctggcgcc agcgcgcgaa agccaacgcg gttatctagt
     3301 ttcatagaca cctcccgcca aggtgagatc ctgagcttgg tattaacctc tccggccggc
     3361 cccgattata gtcttcgcaa atccgaagga aaatgggtta aaaaattatt cccacgctca
     3421 agcttggcca tgactaacgg atggcggtat tcacacgaag gattgttgat cgctcgctgg
     3481 ctgctcgcca aaaccagcgc ggcgcattgg caaactctcg caacgatcga tgaagtccta
     3541 tagatggcaa tcatccaatc gttctcgccg cgcccgtcac cgcctttcat gtgctgtaat
     3601 cgcatttaga atatgctgga caggaaacgg tcgattcgat aggttttcgt cacgccctaa
     3661 gagaaggagg tatctatgat taacgtcgag ggagagccaa tcgaagttat cgatgcacac
     3721 tctcatatgg gcgcgaggaa aaagctcgcc attcatcaga tcccgccgat catgaagttt
     3781 atggctgagg acatgctcga gtcgatggac ggtgccggcg tcgacggagt tgttaccttt
     3841 gcgatcggca tcggggagcc gtcggattat cgagagacca accagtacat cgccgacgcg
     3901 atgaaaaaac atccgggacg gattcacggc tttatgagat tgagtccggg aaacggtccg
     3961 aaggataccc tgcaggtgct cgaggaaggt gtcaagcttg gtctcaaggg gatcaaaatc
     4021 catccgctga tcgaacactg tccggccaac gataaggaaa aagtttatcc gctcatggaa
     4081 gccgcgcagc accacggcct caccgttctc tttcactgcg gcttgggcga ggacgccagt
     4141 ccgaaacgga tcggcgaagt ggccaaagat tttcccaagc tgccggtcat catgggccat
     4201 tcggggctag tcgaaggagt tcgtgaggtt gttgaaatcg ccaaacgctg cgaaaacgtc
     4261 cacatggaca gctccggcgt gggctggttg ccttttttct gcgaatcgat ttcgtgggcc
     4321 ggaccagatc gggtccttta cggctcggat catccgttca atccgatgga ttgggagatc
     4381 gagaaaatcg tcaagcacgc gcagagacat ttaaagctca agatcgaaga tctgcgattg
     4441 atcatggccg gcaatatcaa gcggctgctc aagatgaatt aattcaatta tgaagatcgc
     4501 agcgctggcg gatgtcaaag cccatctcag tgcttatgta gacgaatgcg agaacgaagg
     4561 cccggtcatt atcacgcgca atggcaaggc agctgcgata ttgctggcac ccaagaacga
     4621 cgacgatttg gaacgactga tgttagccca ctcgcgccgt ttgcaatctt tgctcagtaa
     4681 gtcacgaaga agcatcaaag caggcaaggg cgtatcacac gacgaattct ggaagactgt
     4741 cgaccggcgt catcgaggca aacgcccttc acgagcccga aaacgtaaat ccgtcaaata
     4801 gcttgcacct tcccccgcta ggctttttgc catcgaggtt ttcggaatcc caaaacctgg
     4861 aagttcggat cacgattaca gaccgcaacc ccgtttcccc gaccgaacgc ccttgggaat
     4921 cgagaatcgc tatgcggctt aagcggcgcg tccatccgaa aatcgccggg ccaagctgcg
     4981 cgcccggggc cgcgagcgcg agtaagaagt tgtggagttg aattaaaaat gtcttgaggg
     5041 atgaacgaaa caaaaagcaa gaagttctag aggcgaaagg aatctaacct ccatcttaaa
     5101 ctttgtccag ctaggtcaac agcgtcccca tgtctccaca catattcgga aattccgaaa
     5161 acctggaagt tcgacatcgt tctttgtcag caaccaaaca ccatgtctga ttgcaacacc
     5221 tgactgcgga ctccgcgcac attctctgag gtcgtcggcc aggaaacctc atcggcatca
     5281 tgttcaagct cagattaggg cttggcactt acacgtaatc agactcagcc agatccg
//
LOCUS       DASUVS010000382        37527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_821069, whole genome shotgun sequence.
ACCESSION   DASUVS010000382 DASUVS010000000
VERSION     DASUVS010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 37527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37527
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821069"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2335
                     /locus_tag="VF182_20400"
     CDS             <1..2335
                     /locus_tag="VF182_20400"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6439488.1"
                     /translation="SVYRKDASQKVTGQVLHVGNIEMPGMLHVAVLRSPYAHARITRI
                     DKTKAEVVKGVAVVLTGADVAKMPGVDPHFGPAFRDQPILAVEKVCYIGDPVVAVAAQ
                     DRRTAEDALELVEVEYEPLPAVLDVLEAVKAGSPLVHEQHRPAKAFADLAHVKSGEQS
                     NICYHFKLRTGDVERAFAEADRVFEDTFSSPPAQHMPMEPHVTLVYLDEGQRINIWTA
                     SQTPSYVRTEIANTFGVPMNRIRVRIPYLGGGYGAKLYAKLEPLVTALALVTKKPVRY
                     ALTREEEFLTITKHKVITKIKSAIKDGVITARKCEVFWDTGAYAEIGPRVVHKSGYTS
                     AGPYRIPNVWIDSYCVYTNHIPAGAFRGFGVPQVIWAYDSQTDIIARAIGADPVEFRL
                     QHALNEGESFATGTPVRSFGVKQAMREAAKAIEWSEPRLKQSGAKLRGKGIAAGVKAV
                     LTPSISGAIVIMNADGTVNVLSSTVEMGQGSETMMGQIVAEELGISFEQVHVVQPDTD
                     ITPYDTITAGSRSTYHMGNAVRMAAGKVKAELFHVVSKKLEVNPDDLAAGGGRIFVRG
                     TEERGMTISEAFLAKFGSLGTTLTGEAICQPEAIPMDPETGQSEKCTEYWFPSATSAE
                     VEVDTETGHVKILQFYSVGDTGTAINPKHCEQQLLGSAITHLGLTLFEEMIYDEGQLV
                     NGSLLDYQIASFKDLPESFQPIIVEVPHETGPFGAKGVGETGTLTVSAAVANAVEDAI
                     GVRIRDLPITPEKVLRALSQRKPIEGRLHDTSNNFLS"
     gene            2307..2792
                     /locus_tag="VF182_20405"
     CDS             2307..2792
                     /locus_tag="VF182_20405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6439489.1"
                     /translation="MTRQITFCLNGRGETFTIDDADTLLEVLRDRFKLWSVREGCGVG
                     ACGTCTVLLDGKPVSSCFLLAARAAEHEIVTLEGLGDGGTLHYIQEAFINQRALQCAY
                     CTPGFVLSVKAMLDEIPNPSDDDVREYLSGNLCRCAGYADILRAVRAAQDQKKALITR
                     A"
     gene            3004..4896
                     /locus_tag="VF182_20410"
     CDS             3004..4896
                     /locus_tag="VF182_20410"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX6439490.1"
                     /translation="MEVAQTTKSAAFLPNLHNLEELFQSLQKNTVIQLRWPLIILSCY
                     LLLYSPADWLYRSPIQAMVVFYLLTNVTLYFVAEDYFNRPSFYGPLLLFDTFSLALTL
                     GLSGGTTPDLVVACLFTLILSCVCNDPRGLLIITFLAPIVYGYVVLTDAKTADPSIYL
                     RLPLPLVISIFYGYFAQVERQKRIAIEKEQQADRERRTGEEVRVQRARLEVLYRINPA
                     LVGDVGVSKVLDVVLQTTLNHLPYAAAVVTLRNSDTGMLHTAASKGFLNRKSDALVNS
                     LPSLQELIKDQSTLAINNINEDPRVPDIGLQDEGLVGFLGTPLCAQGGVFGGLAFFTR
                     RQYDFSPEEREFATILAGQLAMAIEQTRLHEQIERQANELRDANKLKDEFLGVASHQL
                     KSPLNILARNTNMLIERTLGDISPIQEKSLEMMMRQCKELHAVITSMLQVNFIESNLL
                     EPELHETNLWEFLYDLRSSYDSQLTKDIKLIWEFSPDLPLLEADRGKLKLVLQHVIDN
                     AVKFTDHGYVRVSAHYLANRKVMEFEVSDTGIGIPTDELPMIFEKFSRLDSPDSENYS
                     GAGLGLYIVKRLVHLLQGNIDVQSKVGKGSTVTIRIPARRRKMPPMQEQLTFAMEAES
                     SGPGMG"
     gene            complement(4923..5750)
                     /locus_tag="VF182_20415"
     CDS             complement(4923..5750)
                     /locus_tag="VF182_20415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439491.1"
                     /translation="MAKYRARICPNCRYFLGFSITKMPENDKEVGVISFCLNCSYRLP
                     VHTVLNGFRRATAKLRQNRLKLASSFTVERAADSGHDQTSQRAAGNSSIISPESYSRQ
                     LRAIGQDLQQQGIDSFNLQCSGDHYAVWTRANTVTWPNRSLFNFSNRLENWWGLRNHV
                     PYGYQSRAPVGHARFEYSQDDLARMDVAARIHRHPTSGFANGHSLSQLLRTIGSLAYQ
                     RNHRLLAISWRDISICVVIETAQGKREIEVFRPDNLYDIWVRMYLRRESRALSDVPQ"
     gene            complement(6249..7736)
                     /locus_tag="VF182_20420"
     CDS             complement(6249..7736)
                     /locus_tag="VF182_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEX6439492.1"
                     /translation="MDSTPSKASFSNANRHMQEEEELRLTSVGVDIGSSTTHLVFSRL
                     ELRLEGTRYRVTGREVLKESEILLTPYVDETRIDVEALETFINRQYKQAGIKRDDVDT
                     GALILTGVAVRRRNARAIAELFAEEAGKFVAVSAGDGLEATMAGHGSGAVAHSAKTGG
                     VVLNIDVGGGTSKFAVCNNGKVQEVTAIDVGARLLAFDQDGAVVRIEEAGRKHAGWAG
                     IEVELGQKISQDNLRLVVSGMVEKLFALLKPGPLSDDIKALLRLPPLSYRGEIDSLMF
                     SGGVSEFIYNREKTSFGDLGPLIAEEIHRRMPELGLLVMEPAARIRATVIGASQYTIQ
                     VSGNTIFISPEDAVPVRNIPVVAPEFDFDGEDVDQKAVVAAVQNALRRLDLLSGRQPV
                     ALSFRWRGSATFGRLKSFCSGVAEGLQEILAKGHPLTLINDGDIGGIVGLHFQEELQV
                     DNPIISIDGISLSDFDYIDIGALIPTSGSVPVVIKSLIFPASNDH"
     gene            complement(7825..9021)
                     /locus_tag="VF182_20425"
     CDS             complement(7825..9021)
                     /locus_tag="VF182_20425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6439493.1"
                     /translation="MDHAPEPKWQEPTDNKTRAGLGKFLRPNTPYDNYMEAQGIPIYR
                     DIGVRRVQDLPRKPWKRMGGSGTFIQLLGTEGLWGCFVVEVPGAGALNPQKHLYEEQF
                     LVVEGRGTTEVWAEGNGKKLTFEWQKGSLFAIPMNANHRIINATSSPALLLAGNTAPN
                     IMNNFDNEDFIFNCPYQFKDRFDPSEDYYKYKEDITPDPVRGLAMRQTNIIPDVIACE
                     LPLDNRRSPGYRRIEPHMVGQQFYYWIGQHETGRYSKAHAHGSAAVLICLSGKGYTYT
                     WPATLGTRPWEAGKTELIRRQDYEPVGMVSAAPMSGNWFHAHFGASKEPLRLTAWFGP
                     NAPGRERGRPGEKAIDYGAIDMKDGGTAIPYNEEDPFLRKEFEETLAAVGAKSRMEPE
                     LYTNSY"
     gene            9350..10609
                     /locus_tag="VF182_20430"
     CDS             9350..10609
                     /locus_tag="VF182_20430"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX6439494.1"
                     /translation="MHEAHSFAPLLIVVILASLVPLLLSSVRGRLAIPIVVGEIVAGM
                     IVGRSGFGWVVPESGALELLKELGFVLLMFLSGMEIDFRSLRRADAVQTHHSHWRPLP
                     LAILNFCVTLLLAGAFACGLKWLGATTDPWMMGLILSTTALGVVVPILKEAGVNQTLF
                     GQTILLATVIADFVTMFLISVLVAVISRGLTAEILLVFLLFVAFFFAARFGSFLSRIE
                     PLQRIIQELSHATAQIKVRAAFSTLLIFVVLAEKLGIEIIVGAFLAGAIVALLRTPED
                     RELSSQLEAIGFGFFIPIFFIMVGVDFNLPALVSSSEALLSVPLLLFAAIAVKIIPAL
                     VFRLNHTWREAFAAGILMCPRLSLIIAAAAIGEKLGIIDESVNAAIVLVAVVTVTASP
                     LGFLRVFPAPRAKVARPPVTDTGLAAP"
     gene            10858..12042
                     /gene="solA"
                     /locus_tag="VF182_20435"
     CDS             10858..12042
                     /gene="solA"
                     /locus_tag="VF182_20435"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HEX6439495.1"
                     /translation="MNNKYDAVVVGLGAMGSAAVYQMAKRGRKVLGIDRFTPPHANGS
                     SHGESRIIRQAIGEGEQYVPLVLRSYELWREIEQETGRELLTITGGLTLESRNSEGVL
                     HGRRDFLSQAIRCAEKFAIRYEILETAEIRKFYPQFVVNDERAYFEYETGFLRPEFCI
                     QAQLDLARKRGAAVQNEEVVVSIETDGASAVRIKSSRGVYFAEKVVLSAGSWIASFLP
                     PHYAHLFRVYRQIMYWFDITENHRSTFAPPGFPIFIWIFGIGSEFGFYGFPTLDGKTL
                     KIATEQFTATSDPDQVQRTVSRKEEQEMYKDYVEGRLPGISERCRAAESCLYTVTTDS
                     NFVIDVHPDDDRIIVASPCSGHGFKHSAAVGEALAEQVVEGKSTIDISSFSLKRFKDP
                     VL"
     gene            12137..12931
                     /locus_tag="VF182_20440"
     CDS             12137..12931
                     /locus_tag="VF182_20440"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6439496.1"
                     /translation="MTPVAQFAGRAELPGVRLWYTDSGGTGIPVVLLHANTGNADGFQ
                     YNIRAFADAGYRAIAFDRRGWGRSMLNPDTGPQPGTIAEDLHALVEHLKLDRFHLVGV
                     AGGGFAAYDYVLWHPERLRSLVVAASGGAIIDEELTKLREKTTLPGFRSWPPEFREVS
                     MGYMATNPEGLKRWLGIHNHSQQQGAPAQPQRTTITFDKLATIRVPTLLMPGDQDIQC
                     PPWVMRRQLAHIRHAEFIIIPEASHSINWEQPEAFNRNVLEFIRKY"
     gene            13209..14894
                     /locus_tag="VF182_20445"
     CDS             13209..14894
                     /locus_tag="VF182_20445"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6439497.1"
                     /translation="MKNQFDGGEAIVEAFRKLGVEYILSSPGTEWAPVWEAMANQIHR
                     RKDGPKLLDVWHETLAVNMAAGYTMATGRMQGVLLHAGAGLLQGAMGIHGAFVTGVPM
                     VVISGESMTYGEQPEFDPGAQWINNLSIVGGTTALVDSIVKYSSVAGSAYTLYESVIR
                     AGELAQRQPCGPTYLSVSTETLMDAWTPPANARTVPPAPRVLTAPEDIEKLAAQISKA
                     KHPVVLTEGAGQEVETYEALVALCEKFALPLVEKPGALFANFPKDHPLYQGTDIKPFW
                     NDMDLAIVVRARAPWYPPSNRPPKAVIAMVDEAPHNSAMVYQSHQADMYLEGNAAQTL
                     RDLATTAKVDKAAVDRRRQELGAAHDALVEKRNAAQVAARKKTPIDPVWACAALNAVM
                     PKDSIYIDEVTTHTATLRQHLVWNQPQSLFSRQGGLGQGLGLSLGIKLGKPERQVVTV
                     IGDGAFLYNPVLGSLGAARDYKLPILMVIFNNKKYAAMQGMHQKMYPDGIAAEIDLYH
                     GTHITAPDFVKVAESVGGYGEQVTDPEKLQDALKRGLEANGSGKPAIIDVIVE"
     gene            15449..16474
                     /locus_tag="VF182_20450"
     CDS             15449..16474
                     /locus_tag="VF182_20450"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6439498.1"
                     /translation="MKIQMSRLKVRAWIFLRSNLPVISAISRSGFCIPAILLMTLMGY
                     SLGCASRFPATEVTTIKAATPAELERYLLSRKPDVAQFRSRGPFAFMERRDLEIPLLS
                     GLTVQADLYLCAATRKAPLVIVLHGHDNSKDDHAFQAMHLASWGMHGLAISLPNRGPW
                     IANGRTLAKLVEAIRRTPQLVDDRIDPNNMILVGHSFGATAVAAALGEGAPVLGAVLL
                     DPAGIGRQLRQLLRKITVPVMVIGADDDIWPTRNRAQFYRFIPAGVGEISIRDTTHED
                     AQYPNEYTLRAFEDRPDDTEEAQITFVSALTASAFSLAATGSIDYGWNSFDVGFKKGI
                     FFNGRRK"
     gene            16480..17391
                     /locus_tag="VF182_20455"
     CDS             16480..17391
                     /locus_tag="VF182_20455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6439499.1"
                     /translation="MGVFEPLALRGKTVRIGRTVLIVIVLQVLGLKVGRTQEPAQLAV
                     DDEIAKQEKIYRSRGSDVPSGYVTNRALSDYGEILPSGFCNALGNLGSSDRWLDIGAG
                     AGQAILDYYAPEDDAPPAERCARSAARASSVAISIEDRRTDKWQQQAARLGPERLRYL
                     FGKRLRNYSDEELGKFQIITDVYGGFSYTEDLSQFVDKVLRLLDTGGIFYTLVQRVHL
                     QDGKDKPKAWYQTELVDARGHDVKVCSWLRNTTCALVGCESKSEWDAPTELISIQKIC
                     SDVSVPRMKLLKYEAGNPPSRLFQIEP"
     gene            complement(17530..18576)
                     /locus_tag="VF182_20460"
     CDS             complement(17530..18576)
                     /locus_tag="VF182_20460"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6439500.1"
                     /translation="MDVAIWRGAKIIVATAFLLITAIPAAAQEFFKGKNVVVLVGTAA
                     GGGFDTYSRTLARHLGKYLPGNPSIVVQNMPGAGQLIAANHLYNRSAPDGLTIGHFSG
                     SVIFRHVLGDKSAMFDGRKFGWLGTPAPERHTCVVTEKTNVKTLPDWSASKRPLKFGA
                     LGPGNSTSAWPHLLKSTLGVPLSVIEGFKGTSDIRLAAESGEVDGACWGWDSIKVTWA
                     KGLESGFVRPLIQTTLEPHPDLPQVAVAIQHAKSKEDQALLRLAAQAYGPASIAYSVP
                     PGMAKDRFETLQIAFMDTMNDADFLAEAKKSNLVVSPLDGSTIEQTVAELYRAEPKLL
                     ERFRNVIEMSQPQR"
     gene            complement(18625..19149)
                     /locus_tag="VF182_20465"
     CDS             complement(18625..19149)
                     /locus_tag="VF182_20465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6439501.1"
                     /translation="MSNIARHPRHPAVIKMNLEEIAERYTVRFADIKPDWSVFGDSNY
                     DGYRRAQFRYIGQTLGKPEVRHIPAGATRLSVMFVPPGEGNAPHTHPVEEVFFILKGN
                     VTVFFEEEDGRRVEENLGPWDCVSCPAGVIHGFQNNGLEPAYLQVMVGQTKPEPMGYA
                     DVKLDASDAHLADK"
     gene            19527..21809
                     /locus_tag="VF182_20470"
     CDS             19527..21809
                     /locus_tag="VF182_20470"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6439502.1"
                     /translation="MKSRRKLFSVGTSARRVDGAPKVTGATRYTADHSLPGLIWGKCL
                     RSPLPHARIRRLDIEKAKNLKGVFAVLTAADLPDRLTGIILKDMPVLARDRVRFIGER
                     IAVVGAVSSDVAEEALSRIEVDYDELPAVYDPLQAMAADAPLIHEQLRSYDGLRPPLP
                     DVPNLHNYVEWRSGDYRQGFAEADFVFEQSFTTQRAHQGYLEPHAVVVQVELSGKVMI
                     WSTAKQVYLTRANLAAWLGVDEEKVIFQVGAVGGDFGGKGTLMDLPLCYSLAKVTGRP
                     AKMIMSYAEELSAANPRHASVITIKTGVKKDGRLWARELKAVFNGGAYAGIKESSNGN
                     LPGSRHGNGSYAIPHSYIQSLCVYTNCIPGGIMRGPGDLQVNFAVESHMDYVARRLGI
                     DPYQLRRLNVLKRGDLLVNGQRVNTDMGQRLLEKMRRKIQPRAQARKIPYVGRGLALA
                     SRDVNFGAANIDVGVSENGAAYILTTVPDTGTGAHTIFRQIVAEALTVSAADVAVTMG
                     TTEMFPTDVRVIASLVTYLSGQAVQQAAFALRDKLILRAATVLGCSPHVVRLERGAAV
                     GPKRGKVNFADLAARAKSNDEYLSARGSIDIKERLGAMCFFAEAAEVNVDPDTGQVKV
                     CKVISGHDVGTIINPLMHRGQVEGGMIQGFGFAMSEDLTTEEGKVVTANLGEYKLPTI
                     RDIPEHEALYIRDHTGPGPFQAKQIGEHGAIPTAAAIANAVYDAIGVQITDLPITAEK
                     VFAALRQGPAAQRANAASVR"
     gene            21863..24124
                     /locus_tag="VF182_20475"
     CDS             21863..24124
                     /locus_tag="VF182_20475"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6439503.1"
                     /translation="MIGPRTEIGRSVTRLDGQAKVSGGMRYAADIQRPGMIWGRCLRS
                     PYAHARIRSIDTVAAKDIEGVCAILTADDLPRRLVGRRLKDMPVLAQQRVRFIGERVA
                     VVAAQTPEIAEEALSRIVVDYEELKSVHDPIEAMTADAPIVHENLRSYEILHLPLPDI
                     PNVHSHVQSVVGDVAQGFQESELIFEQTFTTTRVHHGYLEPHAVLVDIEANGRILVWC
                     PAKGPYVTRSHLAQWLGLPEENICFQISPVGGDFGGKASLMDIPLCYSLAKTTGRPVK
                     MIMSYSEELTAANPRHPSLITIKTGLKRDGRLWAREVKVIFNSGAYAGFKNSDNVNLP
                     GARHGPGPYHIPHLKIDAFSIYTNCVPSGIMRAPGEAQMLFAVESHTDFLAAQMKMDP
                     IQFRYRNLLKRGDLLPYGMRLQDDKARQLLKRIAAKIRWNRSRKRQPYVGQGLAFCIR
                     EIGVGEANVEVGLKIDGGVYLVTTVPDTGTGAHTIFRQIAAETLGVAADDVEIVMGTT
                     DHFPTDVAVAASRVTYLAGQASQKAASGLLDLVLHQAAETFRCPVERLRNGKSILRNG
                     AKQKISFAELAARAAAAGRKLQVKANFKIQERGGTACFFAQAARVRVDPETGKVKILK
                     MLSAHDTGTVINPLTYQGQVEGGMMQGLGFALMENLRDEEGKIIAPNLGEYKIPCAAD
                     VPPLETLLFQDQDGPGPFHSKPIGEHSAVPTAPCIANALYDAIGEQITGLPLSAEAVY
                     DAMKTVREARKTR"
     gene            24415..25722
                     /locus_tag="VF182_20480"
     CDS             24415..25722
                     /locus_tag="VF182_20480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6439504.1"
                     /translation="MPVSPLLEQPVKRKPLFYGWVIVFVGFLAHIASAFSISSTLAVF
                     LKPLSADLGISRGTFSLIRSGEILIGAAAAPVVGTLIDRFGGRWLMAAGGLISGAGFI
                     CLGQARDFTQFMLVRWLLISPGDSLMGSMVVNVSIARWFVRMRGRALAIAGMGHGLAK
                     VCMPMAAATLMLYVGWRGSWVVFGFFALLLVVAPALLFMRRSPEEMGLLADGGPVEVR
                     ETAIKPPKRRYAKRTGADNVEWSRGEALRTPVFWLIASTFGVAQIGVTGLNLHVFSYV
                     TDQGHPTLVAASVMSIIAVMQFSTPIVWGMLADRMDNGILNMAKCALQGAGLLLALSL
                     PGMAPLYAGFFIYGIGMGGTAILAETIWADFFGRISLGKIRGMGSLVTSLFSAGGPPF
                     FGLLFDYTESYYLSFSIFIAMLFVSAVLSLCLRPPTKRLEVTV"
     gene            complement(25827..26471)
                     /locus_tag="VF182_20485"
     CDS             complement(25827..26471)
                     /locus_tag="VF182_20485"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6439505.1"
                     /translation="MEIALYYAPHTCALAPYVTLTEAGAKFGVRPLNFRKRQNFSPEY
                     LKINPKHKVPALVVDGKILTENVAIHQWVHQTFPDAKILPSDPWDELQAISLHSWCAS
                     GIHPYLSRINNPAKVCESGGAYDSVVKIATDFLVEAFQIGDDLLAGRDYFFHHFTAPD
                     AHFFWCCRRATQLGVSISDFPNVAAHFKRMQERASVKKLLAYEKEVNEEFAKTA"
     gene            26769..27365
                     /locus_tag="VF182_20490"
     CDS             26769..27365
                     /locus_tag="VF182_20490"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX6439506.1"
                     /translation="MSTTTERPLADALTAIKTRRSVKEYLDTEIPRQWIEELLDAAHW
                     APNHKLTHPWRFHVFTGDGRERLVEARQAAVRAAAEKKGEPVSEDALNFARAKCFSSP
                     VVIIVSMMGDEKKIVDQENYAACWCAIENLLVAANARGLGSYPSTGDWIDQNFVGPVL
                     GLTEKERPVACIFLGYSEQKTMAKRLPVERHTTWYTEP"
     gene            27981..29006
                     /locus_tag="VF182_20495"
     CDS             27981..29006
                     /locus_tag="VF182_20495"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439507.1"
                     /translation="MRYRSRPWTLCLSIFVILCCCGLGHVSANDVVLMSYGGHNETIG
                     PYWVAVDKGLYQKHGIDGRLLQVRSAQISLSALISGEVPIFLPSIANVLSGVSAGAKL
                     GCIAFPIKGVSRELVVRRDIDSLAALRGKTIGVQSIGGGFWLQTMMLLDAAGVDPDKI
                     GMKTRVIGEEATIVQALVSGNVDAAVVTLPSAQVAKRGGARTLVTTADFPIVLQTVGL
                     CGRNERIANSPELMMRLAKGMVDGIVYILDPRNKIEVSAILKKHLRLSADEIAETSYH
                     SLRTIISLDIAPDVAAFKNVQRIAARVNPKIAQVDVNEAINLNVVKALENSGYLAEAR
                     RKVALTR"
     gene            complement(29432..30532)
                     /locus_tag="VF182_20500"
     CDS             complement(29432..30532)
                     /locus_tag="VF182_20500"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6439508.1"
                     /translation="MKDQSKIIDGDGHVFEDNQAIARHFPYGVETGRITFRNGLFPAN
                     SHMQYSLTRTPPGAFGAGADGQFHNPGPEGWLQFLDQTGFEYTVLFPTHGQRIGRIVD
                     RDYAFGAARAYNDWLAEAYLQRDPRFKGIALIPMHDAEAAVEELNRAYTKLGFSGVLF
                     PATAVQLNLGAKPYWPVYQEAARLGCPVVVHGGGHWDLGMDSMNISTGANAIGHPMSL
                     AIALTEMLLNNLFERYRGLRVAYLEGGPLWFLMAIARLTRSFEAGVPINPRGELLQLP
                     QGQTVGDYVRKLVQDGRLVVGIEGGESDLPYAIKVAGEGAFIFSSDFPHEVNTHTVQK
                     EIRELRENEEISAPAKDAILYGNAARFYGLKH"
     gene            complement(30758..31618)
                     /locus_tag="VF182_20505"
     CDS             complement(30758..31618)
                     /locus_tag="VF182_20505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX6439509.1"
                     /translation="MIEKTYWSALLLTAVLVWSDTNLFQVHAQTQPGKGLSPDEERII
                     QKAKEEVLKELRESDFLKEQIQLGIQEYVKKQQEAQVAAKLKQVRIANEMAQNVRPVS
                     VTRDHIYGNSNAPISLIEYSDFECPFCKSFHPTAKAIVKAYGGKVNWVYRHFPLSFHN
                     PGAQKQAEAAECANEFGGNEAFWKYTDSIYERTTSNGKGFPLSELVPLAKEIGLDENR
                     FTRCLESSKYAARVKEDLDEGAKIGITGTPANILFHHETGTAVVKTGAQPLESFKPDI
                     ERMLKETTSK"
     gene            31936..32673
                     /locus_tag="VF182_20510"
     CDS             31936..32673
                     /locus_tag="VF182_20510"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6439510.1"
                     /translation="MTTNYDQIAAEYKRAKQQPWRLHLEHYTMFKLLGDLSGKTVLDL
                     ACGEGFYTRFIKRQNAARVVGVDRSAGMIALANEEEERHSLGIEYLVQDVKQLALDET
                     FDIVTSGYLLNYAESAAELSAMCAAITRHLKPGCRFVTVNNNPRHEPEYFAATRKYGF
                     VKKAERSLTEGPPVDYVFYLDEGSFAITNYHLSIETHELALKSAGFRQVTWHDPKLSA
                     DVADPSARQYWAEFLAHPPVVFIDCIK"
     gene            32905..33867
                     /locus_tag="VF182_20515"
     CDS             32905..33867
                     /locus_tag="VF182_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HEX6439511.1"
                     /translation="MTTSFETKPKLGISACLLGQKVRFDGGHKREQFLTDLFGRFVDW
                     IPICPEIEAGMGVPRESVRLVGTSADPNMIAEKSGRDWTAAMNQFAAKRLRGLAAANL
                     SGYVFKKNSPSCGMERVKVYSSKGMPERQGRGLFAAAVMKHWPFLPVEEEGRLNDLKL
                     RENFIERVFAYHRWQQAIAGRKSLAALVRFHTSHKYLLLAHSQPHYRRLGRLVATAKQ
                     KSLGAVYDAYGRMFMQALAVHATEKTHANVLDHLMGYFSKQLTAAEREELVQLISDFR
                     HQLIPLIAPITMIRHYTKKYRVEYLLGQVYLEPNPKELMLRNHV"
     gene            34212..35546
                     /locus_tag="VF182_20520"
     CDS             34212..35546
                     /locus_tag="VF182_20520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6439512.1"
                     /translation="MSDIPGIERSGKERRRIFFGWYMVAASVAANTIFSAAYFQGFGV
                     LILPIERTFGWDRWVISAAMSLRQLESGIVSPAVGFLLDRFSARKLIFWSAVISGTGF
                     IGLGFTSGIVMFFLCFVIVSLGASGVSHAVTWPVIISRWFRRNRGLATGLAVTGPIFG
                     SPIVILNTQIEEAYGWRIVLFGYGILILVGITLLSMLVRDRPEPYGLRPDGDPPEDSA
                     ATEDLNVPSRRRIDAGLTLHAVFRTKEFWLFTSYLSGTFAVNSAVQGHMIPYFQQDIG
                     LSAAWAAVVMSMVFIISGIGRIGGGYLLDRMDYRLVLAVVAAMMGLGLLYLQVVPVKS
                     VWATLPFAVMFGVSFGCLVPMRGTVGSIMFGTRTIGGILGLLQGGPIAAGVIGPLVMG
                     LIFDLHGRYSTAIWGLIVISALMIPLSLAMGSPAELAKRIRAADSDTRVDLH"
     gene            complement(35679..36629)
                     /locus_tag="VF182_20525"
     CDS             complement(35679..36629)
                     /locus_tag="VF182_20525"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX6439513.1"
                     /translation="MPSLHERFSGLFIPHVTPFDQSGAIDIESLTRLSAYFASRPGVA
                     GLVSCARIGESPVLSIEEKRRVYELSGKVAHEAGKVHIATIAPQSTDEAVALLRDLEH
                     IPVDGVMIFPPLLFAWGNVDPNLKVRFFEDLERETKLPIVLFQIPVKSYYYDPATICR
                     IARLENVVAFKEASFDINLFRDTIGALERDNAQMRVLTGNDRFVAESYKMGADGALIG
                     VANVATEKWGALDLAGRAGNFDQAAAIQSELESVKELVFSEPIVEAVARIKAVLTHEG
                     LIKTSFVRRPQLGISEAEKKQLIDNYNLLKNNKFRAAANQ"
     gene            36795..>37527
                     /locus_tag="VF182_20530"
     CDS             36795..>37527
                     /locus_tag="VF182_20530"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6439514.1"
                     /translation="MNARISCAELARLMESNERFAVFDVRERGEYNQCQIPNATSLPR
                     SQIEFRIAQLVPDLKVRLIIYDEGGARAPLALSTLVRLGYEHVAILDGGLSAWQSHGR
                     LTSSGVNVPSKAFGERVHHERNVPDLAPEELKSLQDRSTDLIILDVRTPEEYARFCIP
                     GGINVPGGDLILWAEELKQRKGTNVIVNCAGRTRSIIGAAALRRLGVGNVRALRNGTM
                     GWVLAGFELEDKPERTPPPAPATDAE"
BASE COUNT         8116 a        10358 c        10683 g         8370 t
ORIGIN      
        1 gtcagtatac cgcaaagatg ccagtcaaaa ggtcaccggg caggtgttgc atgtcggcaa
       61 tatcgaaatg ccgggcatgc tacacgtggc tgttttgcgc agcccatacg cgcatgcgcg
      121 tatcacgcgc atcgacaaaa ccaaagccga ggtcgtcaaa ggcgtggccg tggttctgac
      181 cggcgccgat gtcgccaaga tgccgggtgt cgatccgcat tttgggccgg cttttcggga
      241 tcaaccgatt ctcgcggtcg aaaaggtctg ttacatcggc gatccggtcg tcgccgttgc
      301 cgcccaggac cggcgcacgg cggaagacgc gctggagctg gttgaagtcg aatatgagcc
      361 gcttccggct gtgctcgatg ttctggaggc ggttaaagcg ggctcaccgc tggtgcacga
      421 gcagcaccga ccggccaagg cttttgccga tcttgcccat gtaaaatccg gcgagcaatc
      481 aaacatctgc tatcacttca agctcagaac cggcgatgtc gaaagagcat ttgccgaagc
      541 ggaccgggta ttcgaagata cgtttagctc gccgccggcg cagcacatgc cgatggagcc
      601 acatgtcacg ctggtttatt tagatgaggg gcagcgcatt aatatctgga ccgcgagcca
      661 gacgccgtcc tacgtgcgca cggaaattgc caacacgttc ggtgttccga tgaatcgcat
      721 ccgcgtccga attccgtatc tcggcggcgg ttacggagcc aagctttacg ctaaattgga
      781 gccgctggtc accgcactgg cgctcgtcac caagaagccg gttcgttacg ctttgactcg
      841 ggaagaagaa tttctgacaa tcaccaaaca caaagtgatc accaagatta agagcgccat
      901 caaagacggt gtgatcacgg cgcgcaaatg cgaagtcttc tgggataccg gcgcgtacgc
      961 cgaaattggc ccgcgggtcg tgcacaaatc gggctataca tcggccgggc cgtatcgcat
     1021 tccaaacgtc tggatcgatt cctattgcgt gtacaccaat cacatacctg ccggcgcgtt
     1081 tcgcggtttt ggcgtgcccc aggtgatttg ggcttacgat tcgcaaaccg acataatcgc
     1141 gcgcgccatt ggggccgatc cggtggagtt tcggctgcaa cacgcgctga acgaaggtga
     1201 gtccttcgcc actggaacgc cagtcagatc gtttggcgtc aagcaggcga tgcgagaagc
     1261 ggccaaggcc attgaatgga gcgagcccag gctcaaacaa tccggcgcca agctgcgcgg
     1321 taaaggaatt gccgccggcg tcaaagccgt gctcacgcct tcgatctcag gcgcaatcgt
     1381 gattatgaac gccgacggca cggtcaacgt gctcagcagc accgtcgaaa tgggtcaagg
     1441 ctcggagacg atgatgggcc agatcgtcgc cgaagagctt ggcatttcct tcgagcaggt
     1501 gcatgtcgtg caacccgata ccgatatcac tccgtacgat accatcaccg cgggcagtcg
     1561 ctccacttat catatgggca atgccgttcg catggccgct ggaaaagtca aagccgaatt
     1621 gtttcatgtc gtctcaaaaa aattggaagt gaatcctgac gatctcgcag cgggtggcgg
     1681 gcgcattttc gtgcgcggca cggaagagcg gggcatgacg atcagcgagg catttctcgc
     1741 caagttcggc agtctgggca cgacactcac cggcgaggcg atctgccagc cggaggcgat
     1801 tcccatggac ccggaaaccg ggcagtcgga gaaatgcacc gagtactggt ttccttcggc
     1861 gacctcggcc gaggtcgaag tggacaccga gaccggccac gtcaaaattc ttcaattcta
     1921 tagcgtcggc gataccggca cggccataaa tccgaaacac tgcgagcagc aactgctcgg
     1981 ttcggcgatc actcatttgg gcttgacact ttttgaagaa atgatttacg acgaaggaca
     2041 actggtgaat ggttctttgc tagattacca gatcgcttcg ttcaaggatc tgccggagtc
     2101 ttttcaaccg attatcgtcg aagtgccgca cgagaccggt ccgttcggcg ccaagggcgt
     2161 cggcgaaacc ggcacgctca cggtttcggc ggctgtcgcg aacgccgtcg aggatgcaat
     2221 tggggtccgc attcgcgatc tgccgatcac gccggaaaaa gttttgcgcg cattgtctca
     2281 acggaagccg atcgaaggta ggttgcatga cacgtcaaat aactttttgt cttaacgggc
     2341 gcggggaaac gttcaccatc gacgacgccg acactttgct cgaggtactg cgggatcgct
     2401 ttaaactatg gagcgtgcgc gaaggttgcg gcgtcggcgc atgcggcacc tgcaccgtgt
     2461 tgctcgatgg taaaccggta agctcgtgct tcctgttggc cgcgcgcgcg gcggagcatg
     2521 agatcgttac cctggaaggt ttgggagacg gcgggactct tcattacatc caagaagctt
     2581 tcatcaatca gcgagcatta cagtgcgcat actgcactcc tggtttcgtt ctctccgtca
     2641 aagcgatgct cgatgagatc cccaatcctt cggatgacga tgttcgcgaa tatctgtccg
     2701 gtaatctgtg ccgatgcgca ggctacgccg atattctacg ggccgtccgc gccgcgcagg
     2761 accaaaaaaa ggcccttatc acccgcgctt gatctgctgg aaagagcccc tgtctcgccc
     2821 atctcgccgc acccctacgt acatcaacgc ggcgacaaat ttgtccccat ttgttgtaat
     2881 ttgtccattt cagatcggcc gtgctttgaa gacaccgccg caactcgccg atcttatgta
     2941 acaaactgaa gaggcaatac aaattaccat ggcatataat gtgcaaccac atggaaagag
     3001 tttatggagg ttgcacaaac taccaagtcc gctgcgttct tgccaaacct ccataacctt
     3061 gaagaattgt ttcaaagcct gcagaagaac actgtcattc aactgcgctg gcccctgatc
     3121 attctctcct gttaccttct actctactcc ccggccgatt ggctttaccg gtcgccgata
     3181 caggcgatgg tggttttcta tctactgact aatgtaacgc tgtattttgt cgccgaagat
     3241 tacttcaacc ggccgagttt ctatggcccg ctgctgctgt tcgatacttt ttccctcgcg
     3301 cttaccttgg gcttgagcgg cggcaccacg cctgaccttg tcgtcgcttg cctgttcaca
     3361 ttgatactca gttgcgtttg caacgatccg cgcggcttat taatcatcac gtttttggct
     3421 ccgatcgttt atggctatgt cgtgcttacg gatgcgaaga ccgcggatcc aagcatttat
     3481 ttgcgccttc cattgccgct ggtgatctcg atcttctatg gctacttcgc acaggtagag
     3541 cggcagaaaa ggatcgcaat cgagaaggag caacaagccg atagagagcg tcggaccggc
     3601 gaagaagtgc gagtacagcg cgcgcgcctg gaggtacttt atcggatcaa tccagcgctc
     3661 gtcggagatg ttggagtcag taaagttctc gatgtagttt tacaaacgac actgaatcat
     3721 ctcccctatg cggcggccgt ggtcacgttg cgaaattctg atacgggcat gcttcacacc
     3781 gcagccagca aaggattcct caatcgaaaa tcagatgcgc tcgtcaattc attgccaagt
     3841 ctccaagagc ttataaaaga ccagagcacg cttgcgataa acaatatcaa tgaagatccg
     3901 cgggtcccgg atatcggcct gcaagatgaa gggctggtcg gatttctcgg gacgccgctg
     3961 tgcgcccaag gcggggtttt tggcggctta gcatttttca cgcgccggca atacgatttc
     4021 agcccggagg aacgggagtt tgctacgatc ttagcgggcc agctggcgat ggcgatcgag
     4081 cagactcgct tgcacgaaca gattgaacgc caggccaacg aattgcgcga cgccaacaag
     4141 cttaaggatg aatttctcgg cgttgcttcg catcaattga aaagcccgct caacatactg
     4201 gcgagaaata ccaacatgct gatcgaaagg acgctcggcg atatcagtcc gattcaagag
     4261 aaatctcttg aaatgatgat gcggcaatgt aaagagcttc atgcggttat caccagtatg
     4321 ttgcaggtca atttcatcga atcgaatctg ctcgagcccg agcttcacga aaccaactta
     4381 tgggagtttc tctatgacct gaggtcctcc tatgattctc agttgaccaa agacataaaa
     4441 ttgatctggg agttttctcc cgacctgccg ttactcgaag ctgatcgcgg caagttgaaa
     4501 ttggttctgc aacacgtcat cgacaacgcc gtcaagttca ccgatcatgg ctacgtcagg
     4561 gtttcagcgc actacctggc caatagaaag gtgatggaat tcgaagtgtc ggatactggt
     4621 atcgggattc cgactgacga gctgccgatg atttttgaaa agttcagccg gctcgatagt
     4681 ccggattccg aaaactacag cggcgcgggc ttagggctat acatcgtaaa acgacttgtg
     4741 cacctgctcc aagggaacat tgacgtgcaa agtaaagtcg gtaagggatc gaccgtgacc
     4801 atccggattc cggcgcggcg gcgaaagatg ccaccgatgc aggagcaatt aacttttgcc
     4861 atggaagcgg aaagttccgg acctggaatg ggctgagtga tatcaacccg gaagggaagc
     4921 cgtcactgag ggacatccga tagcgcgcgg ctttcgcggc gcaaatacat tctcacccaa
     4981 atatcgtaca agttgtccgg acgaaaaacc tcaatctctc gttttccttg ggcggtttcg
     5041 ataacaacgc agatcgatat gtcacgccat gaaatcgcca gcaagcggtg atttctttgg
     5101 tacgccaaag agccgatagt cctgagcaac tgtgataagc tgtgcccgtt ggcgaatcca
     5161 ctcgtcgggt gacggtgaat tctcgcggcc acgtccatgc gcgcaagatc gtcttgagaa
     5221 tattcgaagc gcgcatggcc gacgggcgcg cggctctgat aaccgtatgg cacgtgattc
     5281 cttagacccc accagttctc cagccgattg ctaaagttga agagtgatcg attcggccaa
     5341 gtcacggtgt tggcgcgggt ccaaacggca taatgatcgc cgctacattg caggttgaaa
     5401 ctgtcgatcc cttgctgttg caagtcttgt ccgatagccc gcagctgacg gctatagctc
     5461 tcgggtgaaa tgatgctgct gttgcccgca gcgcgttggc tggtttgatc gtggcccgag
     5521 tccgcggccc gctcgacggt aaaactactc gcaagcttca gccgattttg cctgagtttg
     5581 gctgtggctc gtctgaaccc attcaggaca gtatggacgg gcagccgata gctgcaattc
     5641 aaacagaagc taatgacgcc gacttccttg tcgttctcag gcattttcgt gatggagaat
     5701 cctaggaagt agcggcagtt gggacagatc cgcgcgcggt atttagccat gatggaggtc
     5761 cactggcggt ttcattattc ctaaccgctt gttatgtcaa gcaccgatgc ggttattctc
     5821 gatcttgccg cttaacaaaa aacttcgcct gcgtaaaact gcagcaaaag ggtcgttatt
     5881 tctacttttg acgatctgtt agatttgccc aacagtgtga atcaaacgcc aatcccatct
     5941 atttctcacg gttttgtatt gctgaacccg ctggcattgc cagcgagaac tgtttgatcc
     6001 gggccgagct gcgaatttag ccgttagtgg tttagggcga acctagcgac actgcaggct
     6061 gctattatta aaagacgggc agacaaaaag agggtgactg agctgccgcc aaaatgcggt
     6121 aatgactcgg ggaggaagaa gggagcttcg ttcgtagcct tacttgtgaa tgaacgctca
     6181 aaacttaatc ggtcgaaggt cttgagcgcc tggaacggtt tgaacggttt gaacgattcg
     6241 aacttttctt aatgatcgtt cgatgccggg aagatcagcg acttgatcac gactggaaca
     6301 gatcctgacg tgggaatcag agcgccgata tcgatgtaat cgaaatcgct gagcgaaata
     6361 ccatcgattg aaatgatcgg attgtcgact tgcaattcct cctggaagtg aagcccgacg
     6421 atgccgccga tatcgccgtc gttgattaac gtcaatggat ggcccttggc caagatctct
     6481 tgaagtccct cggcgacgcc gctgcaaaaa gattttaacc gaccgaaagt cgctgagccg
     6541 cgccatcgaa acgataacgc caccggttgc ctaccgctga gcagatccaa tctgcgcagc
     6601 gcattttgca cggccgcgac gacggctttt tgatcgacgt cttcgccgtc gaagtcgaat
     6661 tcaggggcaa ccaccgggat attgcgaacc ggcacggcat cttccggtga aatgaaaatc
     6721 gtattgccgc tgacctgaat ggtgtactgc gaggcgccga ttaccgtggc gcgaatccgc
     6781 gctgccggct ccatgacgag caggcccaat tcaggcatgc gccggtgaat ctcttcggct
     6841 atcagaggcc cgagatcgcc gaagctggtt ttttcgcgat tgtagataaa ttccgaaacg
     6901 ccgcctgaaa acatcaagga atcgatttca ccccggtaac taagcggcgg caagcgcagg
     6961 agagctttga tatcgtcgga aagcggccca ggcttgagca gtgcgaaaag cttttcaacc
     7021 atacccgaga ccaccagccg cagattatcc tgggagatct tttgaccgag ctccacttcg
     7081 atgccggccc atccggcatg cttgcggccg gcttcctcta tccgtaccac ggcgccatcc
     7141 tgatcgaagg cgagcaggcg cgcgccgaca tcgatggccg tgacttcctg gaccttgccg
     7201 ttgttgcaca cggcgaattt gctggtgccg ccgccgacgt caatatttaa gaccacgccg
     7261 ccggtcttgg ccgaatgcgc cacagctccg gagccgtggc cggccatggt ggcctccagg
     7321 ccgtcgccgg cgcttacggc gacgaacttg cccgcttcct cggcgaatag ctcggcgatc
     7381 gcgcgagcat tgcggcgcct gaccgccacg ccggttagaa tcaacgcgcc ggtgtcgaca
     7441 tcgtcgcgct tgatgccggc ctgcttgtat tgccggttga taaaagtctc gagggcttcg
     7501 acgtcgatcc tggtctcatc gacgtagggg gtcagaagaa tttccgattc cttcaaaacc
     7561 tcgcgaccgg tcacgcgata ccgcgtgcct tcgaggcgga gctcgagccg ggaaaaaacc
     7621 agatgagtgg tggaggaacc gatgtccaca ccaacgctgg tgagtcgcag ctcttcttcc
     7681 tcttgcatgt ggcggttggc gttggagaac gaggctttag aaggggtgct gtccattagg
     7741 tacctgggtc gaaatgggtt gaaactgtgg ctggagcttc ggactctgtg ggaacccgaa
     7801 gctccaccgt tcggatggat tgaatcaata cgagttggtg tagagctcgg gttccatacg
     7861 ggactttgcg cccaccgcgg ccaacgtctc ctcaaattct ttacggagga aaggatcttc
     7921 ttcattgtag ggaatcgccg tgccaccgtc tttcatatcg atggcgccat aatcgatcgc
     7981 cttctcgccg ggccggccgc gctcgcgtcc cggagcattg gggccgaacc aggcggtgag
     8041 gcgcagcggc tccttgctgg cgccgaagtg ggcgtggaac caattgccgc tcatcggcgc
     8101 ggcgctgacc ataccgaccg gctcgtaatc ctgtcttctg atcaattccg ttttgccggc
     8161 ctcccacggg cgtgttccca atgtcgccgg ccaggtgtaa gtgtagcctt tgccgctcaa
     8221 gcaaatcagc acagcggccg agccgtgggc gtgggccttg gagtaacggc cggtttcatg
     8281 ctgtccaatc caataataga actgttgtcc gaccatgtgc ggctcgatgc gccggtaacc
     8341 gggcgagcgc cgattgtcga ggggcagctc gcaggcaatc acgtcaggga tgatgttggt
     8401 ttgacgcatc gccaaaccgc gcaccggatc gggagtgatg tcctctttgt atttgtagta
     8461 gtcctcgctc ggatcgaacc gatctttgaa ctgataaggg cagttgaaga taaaatcctc
     8521 gttatcgaag ttgttcatga tgttgggcgc cgtgttgccg gccagaagca acgcgggact
     8581 cgaagtggcg ttgatgatcc ggtgattggc gttcatcgga attgcgaata gcgaaccttt
     8641 ctgccattcg aatgtcagct ttttgccgtt gccctcggcc cagacttcgg tggtgccgcg
     8701 gccttcgacc accaggaact gctcttcgta gagatgcttc tgcggattga gcgcgccggc
     8761 gccgggtact tcgacaacaa agcagcccca caatccctcg gtgccgagca attgaatgaa
     8821 ggtgccgctt ccgcccattc gcttccaggg cttgcgcggt agatcctgaa cccgccggac
     8881 gccgatatcg cggtaaatag ggatgccctg cgcttccatg taattgtcat agggagtatt
     8941 cgggcggaga aacttgccga gaccggctct ggttttgtta tcagtgggct cctgccactt
     9001 cggttcgggt gcgtggtcca taactcctcc ttctgattcc tgcttgtttg gaactgttcc
     9061 ggtgcgatca aatgcgccag ttcagcggat tgaaataact tataagctgc cgaaatggga
     9121 gtcaaacggc gcgcgaaaaa caagatgaat caagagaaat gaggcgaatg aaggagggtg
     9181 ttcgtgatcg gacataaaac tcatctgcaa atataataga aatgaaaatg gaagcttgag
     9241 aggggccgga ggcgtgaggg agaagcggcg catgatggtg gggtgatctt gcgtagaaaa
     9301 aaaataacga taatagttgt gaattcggtt tacgaaaggc acccgattca tgcatgaagc
     9361 gcactcgttc gcgccgctcc tgattgtcgt catccttgcg tcgctggtgc cgctgctgct
     9421 ctcgtccgtg cgcggccggc tcgccatacc cattgttgtc ggtgagatcg ttgccggcat
     9481 gattgtcggg cgtagtggat tcggttgggt tgtccctgag agcggtgccc tcgagctcct
     9541 gaaagagttg ggtttcgtgc tgctgatgtt tctttccggc atggagatcg attttcgcag
     9601 cttgcgccgg gccgatgccg ttcagaccca ccactcgcat tggcgcccat tgcctcttgc
     9661 catcctgaac ttttgtgtaa ccctgctact cgccggcgcc tttgcctgtg gcttgaaatg
     9721 gctcggagcc accactgatc cctggatgat gggattgata ctttcaacca ccgcgttggg
     9781 agtggtggtg ccgatcctca aggaagcggg ggtgaaccag accttgttcg gacaaacgat
     9841 tttactagcc accgtcatcg ccgatttcgt gacgatgttt ctgatcagcg tcctggtcgc
     9901 ggtgatttcc cgcggcttga ctgccgagat tctattggtg tttttattgt tcgtggcttt
     9961 tttcttcgcg gcaagattcg gctcgttcct gagccgcatc gagcccttgc aaagaatcat
    10021 ccaggagctc agccatgcaa cggctcaaat caaagtccgc gcggctttct ccactttatt
    10081 gatttttgtc gtgctcgccg aaaaactcgg catcgagatc attgtcggcg cgtttttggc
    10141 gggagccatc gtcgcgctgt tgcgcacacc cgaggacaga gagctgtcga gccagctcga
    10201 agccatcggc tttggatttt ttattccgat cttcttcatc atggtcggag tggattttaa
    10261 tctgccggct ttggtgtcct cgagcgaagc ccttctttcc gtgcctctgc tgttgttcgc
    10321 cgcaattgcg gtaaaaatta tcccggcact ggtgttccgg ctcaatcata cgtggcgcga
    10381 ggcttttgcc gccggtattc tgatgtgccc gcggctctcg ttgatcatcg ccgccgccgc
    10441 gatcggtgag aagctaggga tcatcgacga atcggtgaat gcggctatcg ttttggtggc
    10501 ggtcgttacg gtcacggcat cgcctctcgg ctttctgcgg gtgttcccgg cgccgcgggc
    10561 gaaagttgcg cgtcccccgg tgaccgatac gggccttgcc gcgccgtgac gctgcgccgg
    10621 acttcaccgg ctcttcggcc ctcctgcctt gcgccgctag catggcgaca ttggatcgct
    10681 ggcaatgaat acatcgacat ccaacgtgtg aaccctaaat tatcgaacgt cctccatcct
    10741 aacgtttgga agcggagcgc agcggttgaa cggtgaaacg attggaagtg actttggtcg
    10801 tttaatcgct cgcgcctcga gcggtagagt tcgtcttagc ccagcaaaac cagagtgatg
    10861 aacaataagt acgatgccgt tgtagtgggt ctcggcgcca tggggagcgc cgcggtctac
    10921 caaatggcca aacggggcag gaaagtgctc ggcatcgatc ggtttacccc gccgcatgct
    10981 aacggttcct cccacggtga aagtcgaatc attcgccagg cgattggcga aggcgagcaa
    11041 tatgtgcctc tggtcctgcg ctcctatgaa ctttggcgcg agatcgaaca agagaccggc
    11101 agggagctcc tgacgatcac cggcggtttg accctggaga gtcgaaacag tgagggtgtt
    11161 ctgcacggcc ggcgtgattt tctaagccag gcgatccgct gtgcggagaa gttcgctatc
    11221 cgttatgaaa tcctggaaac cgcagagata aggaaattct atccgcagtt tgtggtaaat
    11281 gatgagcgcg cttacttcga atatgaaacc ggatttctaa ggccggaatt ttgtatacag
    11341 gcgcagctgg atctcgcccg caagcgcggc gctgccgttc aaaacgagga agtcgttgtt
    11401 tcaatcgaaa ccgacggggc ttccgcagta aggatcaaga gcagccgcgg cgtctacttt
    11461 gctgaaaagg tcgtcctctc cgccggctcc tggatcgcca gtttcttacc accgcactat
    11521 gcgcatctgt tcagggtgta tcgccagatc atgtactggt tcgatatcac ggaaaatcac
    11581 cgttcaacgt ttgctccccc cggttttccg attttcatct ggatctttgg gataggcagt
    11641 gagttcggtt tctatggctt tccgaccctt gacggtaaaa cgctcaaaat tgcgacggag
    11701 caattcaccg caaccagcga tccggaccag gtgcagcgga cggtcagcag gaaagaagag
    11761 caagagatgt ataaagacta tgtcgaaggc cggctaccgg gaatatctga gcgatgcagg
    11821 gccgccgaaa gttgccttta tacggtgacg accgattcga acttcgtcat cgacgttcat
    11881 ccggatgatg atcgcattat cgttgcatcg ccgtgttcag gtcatggatt caaacactcc
    11941 gcagcggttg gcgaggcgct ggcagagcag gtcgtcgaag gcaaaagcac gatcgatatc
    12001 agcagtttca gcctgaagag attcaaagat ccggtcctct aactcgactc gtttctgcat
    12061 caaatcgatt tgacaagcta gagcgcatcg atgtaatgct cgcacgatca ataaacaagc
    12121 attctcaggc agtgatatga cacctgttgc acaattcgcc ggccgtgcgg aacttcccgg
    12181 cgtgcgtctc tggtacaccg acagcggcgg caccggcatt ccggtcgtgc tgcttcatgc
    12241 caataccggc aacgccgacg gctttcagta caatatccgc gcattcgcgg acgccgggta
    12301 tcgcgccatc gctttcgatc ggcgcggatg gggccgcagc atgctgaatc cggacaccgg
    12361 cccgcagccc ggcaccatcg ccgaagatct tcatgccctt gtcgaacacc tgaagctcga
    12421 ccgctttcat cttgtcggcg tggcgggcgg cgggtttgcc gcttacgatt acgtgctctg
    12481 gcatccggag cggctccgca gtctcgtggt tgccgccagc ggcggcgcca tcatcgatga
    12541 agagttgacc aaactgcgcg agaagacgac cttaccgggc ttcaggagct ggccaccgga
    12601 gtttcgcgaa gtcagcatgg gctacatggc gaccaacccc gaaggcctga agcgctggct
    12661 cgggattcat aaccattcgc aacagcaagg cgcgccggcg cagccgcaac gaacgacgat
    12721 cacgttcgac aaactcgcga cgatccgtgt gccgaccctg ctcatgcccg gcgatcaaga
    12781 catccagtgc ccgccctggg tcatgcgccg ccagctcgcc catatacgtc acgccgagtt
    12841 catcatcatt cccgaagctt ctcactcgat caactgggag caaccggaag ccttcaaccg
    12901 taacgtgctt gagtttattc gcaagtatta gccgaattcc ctgacgcagt tcttattctg
    12961 atctgcgtca acgccgttca aaaaattttg ggggagctcg gggaaaaccg gcgcttcttt
    13021 gtttgatatg attcgaacga cttaaatgga gcgcagcggg tgaacgactg ctgttgaacg
    13081 ggccgcacgc ctactaaccc ttcgtcgcct tcgcgcgact ctaaaatcaa catcggagcc
    13141 ggccaggggt cttgcgtgtt caggcgtgag ataatacaga ccagaagaaa actgttggga
    13201 gaccaaacat gaagaatcag ttcgatggcg gcgaagcgat agtcgaagca tttcgcaagc
    13261 tcggagtcga atacatcttg tcctcaccgg ggaccgaatg ggcgccggtg tgggaagcca
    13321 tggccaatca aatccaccgg cgcaaagacg gtcccaagct gctggatgtg tggcacgaaa
    13381 cactagcggt aaacatggcc gccggttaca cgatggccac cggtcggatg cagggcgtgt
    13441 tgctgcatgc aggcgccggt ttgctgcagg gcgccatggg catccacggc gcctttgtca
    13501 ccggcgtgcc tatggtagtg atttccggag agtcgatgac ctacggcgag cagccggagt
    13561 tcgatcccgg cgcccagtgg atcaacaact tgagcatcgt aggcggcacc accgctctcg
    13621 tcgattccat cgtcaagtac tcctcggtgg caggcagcgc ctacacgctt tacgaaagcg
    13681 tgatccgcgc cggcgagttg gcgcagcggc agccgtgcgg acccacttac ctgtcggttt
    13741 cgacggaaac gctgatggat gcctggacgc cgccggcgaa tgcgcgcacg gttccgcccg
    13801 cgccgcgtgt gctgaccgcg ccggaggaca tcgaaaaact cgccgcccag atctctaagg
    13861 cgaagcaccc ggtggtgctt accgagggag ccggccagga ggttgaaacc tacgaggctc
    13921 tggtggccct atgcgagaaa ttcgctctgc cgttggtgga gaagcccggc gcgctgttcg
    13981 ccaactttcc taaggaccat ccgctttatc aggggaccga catcaaaccg ttctggaacg
    14041 acatggatct cgcgatcgtt gtgcgggcgc gcgccccctg gtatccgccg agcaaccgcc
    14101 cgcccaaggc ggtgattgcc atggtggacg aggcgccgca caattcggcg atggtctacc
    14161 agagccatca agccgacatg tacctggaag gcaatgcggc gcagacgctg cgcgatctcg
    14221 ccactaccgc caaagtcgac aaggccgcgg tggaccgaag acgccaagaa ctgggcgctg
    14281 cgcacgatgc gctggttgag aaaagaaacg cggcacaggt ggcggcccgc aagaagacac
    14341 cgatcgatcc ggtgtgggcg tgcgccgcac tgaatgccgt gatgcccaaa gattcgatct
    14401 atatcgacga ggtgacgact cacaccgcca cgctgcgcca acacctggtg tggaaccagc
    14461 cgcaaagcct gtttagccgc cagggaggtc taggacaggg gctggggctt tcgctgggga
    14521 tcaagctggg aaagccggaa cgccaggtcg tgaccgtgat cggcgacggc gcgttcctgt
    14581 ataacccggt actcggcagc ctcggcgcgg cgcgcgatta caagctgcct atccttatgg
    14641 tcatttttaa caacaagaaa tacgccgcga tgcagggcat gcaccagaag atgtatccag
    14701 acgggatcgc ggctgaaatc gatctctatc atggcacgca catcaccgcg cccgacttcg
    14761 tcaaagtcgc cgaatccgtt ggcggttatg gcgagcaggt gaccgatccg gagaagcttc
    14821 aggatgcgct gaagcgcggc cttgaagcca atggcagcgg taagccggcg atcatcgacg
    14881 tgatcgttga atagatgtga caggagcgcg gtggtcgcga aagcagccag ccgcccttcg
    14941 caggctctct ccgaaatcaa atcgggacgt ggctccggcg ccgaagctta ctcgggagcc
    15001 ggttaaattc gaagtcaccg gaaccggcgc aaaccggccg ctcttccaat ccgacttgac
    15061 ttgctcgcga cgctcgcgcg agtttcagtc gaagctccac cgaagatgac gcagactgtc
    15121 gagcggttca ccgccaaaaa atacgaaccg agcggccgat gttgcctcga tcttaatctc
    15181 accgccggag gaaaagaccg cggcctgccc gggctcgaat ctctggccac gaatgtcgac
    15241 gcccgcaaca ggaggcaatc ccggtatcga ttccctaacg agcggcagtg aatctccaaa
    15301 tcgagagtcg cacccgcata aaacagctcc gagaaagtcc tgaccggcga tgccttgtcg
    15361 aaatgagccg gtaatgaccc ggaggatctt tccctcgcca ttcgctttga ggcagcagtc
    15421 agcgtgcatt ttctctatga tggcaaaaat gaaaattcaa atgtccaggc ttaaagttcg
    15481 ggcttggatc tttctcagga gtaacctgcc ggtaatctct gcgatcagcc gatcaggttt
    15541 ttgcattcca gccattctct tgatgaccct gatggggtat tcactcggct gtgcatcgcg
    15601 gtttccggcg actgaagtaa cgacgataaa ggccgcaacc cccgccgagc tcgagcgcta
    15661 tctactgtcc cgtaagcccg acgtagcgca attcaggtcg cgtggaccgt ttgccttcat
    15721 ggagcgcaga gacctggaaa tccccctgct ttctggtctc acggtgcagg cggacctcta
    15781 tttatgcgcg gccacccgca aggcaccgtt ggtcatcgtg ttgcacggtc acgacaattc
    15841 caaggatgat cacgccttcc aagcaatgca cttggcttcc tggggcatgc atgggcttgc
    15901 gatcagcttg ccaaaccggg gcccgtggat tgccaacggc agaacgctgg ccaaactcgt
    15961 cgaagctatc cgtcgaacgc cgcagctcgt tgacgaccgc atagacccaa acaatatgat
    16021 tctcgtgggc cattcattcg gcgccaccgc cgtcgcggct gcattggggg aaggcgcccc
    16081 cgtattgggc gccgttctgc tggatcccgc cgggatcggc cggcaattgc ggcagttgct
    16141 taggaagatc acggttccgg tgatggtgat cggtgctgac gacgatatct ggccgacgcg
    16201 caaccgcgcg caattttacc ggttcattcc ggccggggtc ggagaaatct cgatacgtga
    16261 cacgactcac gaagatgcac agtatccgaa cgagtacacg ctgcgggcgt tcgaggatag
    16321 gcccgatgat acggaggaag cgcaaatcac tttcgtcagt gcactaacgg cttccgcatt
    16381 cagccttgcg gccaccggaa gcatcgacta tggctggaac agctttgatg tcggttttaa
    16441 gaaaggaatt ttcttcaatg ggaggagaaa ataatcgtca tgggtgtatt tgaaccgttg
    16501 gcgctacgag ggaagaccgt gcgaatcggt cgaaccgtgc tgatcgttat cgtcctgcag
    16561 gtgcttgggc tgaaagttgg ccggacgcag gaacccgctc aattagcggt agacgacgaa
    16621 atcgcaaaac aggaaaaaat ctaccggagc cgtggcagcg atgttcccag cggctacgtc
    16681 accaaccgag cgctctcgga ttacggagaa attcttccct ccggcttctg caacgcgctc
    16741 ggcaatctgg gcagctcgga ccgatggctg gatattggcg cgggggctgg gcaggccata
    16801 ctcgactact atgcacctga ggacgacgcg ccaccggctg aaaggtgcgc tcgctcggct
    16861 gccagggcga gttcggtggc gatttccata gaagaccgcc gcaccgacaa gtggcagcag
    16921 caggccgcga ggctcggccc tgaacgccta cgatatctct tcggcaagcg cctgcggaac
    16981 tattcggacg aggagcttgg aaaattccag atcattaccg acgtttatgg cggcttctcc
    17041 tataccgaag acttgtccca gttcgtcgac aaggtgctga gattactgga taccggcggc
    17101 attttctaca cgttagtgca acgtgtgcat ttgcaggatg gcaaggacaa acccaaagcc
    17161 tggtatcaga ccgagctggt cgatgcaagg ggccacgacg tgaaagtgtg ttcatggctc
    17221 aggaacacta cctgcgccct agtgggctgc gagtcgaaga gtgaatggga tgcgcccacc
    17281 gagctgatca gcatccaaaa aatttgtagc gatgtctctg ttccgcgcat gaaattactg
    17341 aagtacgagg ccgggaaccc tccctcgcgt ctattccaaa tagagccgta gagttatgaa
    17401 acagagatcg cggttcgcaa ataggaaaga ttggcggccg ctcatcaaac acaagcgcat
    17461 cccaacaggc gccgcgttca tcgtgaaatg gcgggagagt ctacagattg cggacggcct
    17521 cctgtcgcgt cagcgctgcg gctgtgacat ctcgatgaca ttgcggaagc gctcgagcag
    17581 cttgggttcc gcgcggtaga gttccgccac cgtctgctcg atcgttgaac catcgagcgg
    17641 gctgactacc aggtttgact ttttcgcttc ggccaagaaa tccgcatcgt tcatcgtatc
    17701 cataaaagct atctgcagag tttcaaagcg atccttggcc atgcccggcg gcacagaata
    17761 agcaatcgag gcggggccgt aggcttgtgc cgcaagccgc agcaacgcct ggtcctcttt
    17821 gctcttggcg tgctgaatcg caactgcaac ctggggtaga tccggatggg gctcgagcgt
    17881 ggtctgaatc aagggccgga caaatcccga ttcgagtcct ttcgcccagg tgaccttgat
    17941 cgaatcccaa ccccaacatg cgccgtcgac ttcgccggac tccgccgcca ggcgaatgtc
    18001 cgaagttcct ttgaaccctt cgatgacgct tagcggaacg cccagtgtcg acttgagcag
    18061 atgtggccag gccgatgtcg aattaccggg cccgagcgcg ccgaacttga gcggtcgctt
    18121 ggatgccgac caatccggca acgttttcac attggttttt tcggtgacca cgcaggtgtg
    18181 gcgctccggc gcaggcgtgc cgagccagcc aaattttcta ccgtcgaaca tcgcactttt
    18241 atcgcccaac acatgacgga agatcaccga gccggagaaa tggccgatgg tcaaaccgtc
    18301 aggcgccgat ctattgtaca aatgattggc ggcgatgagt tggccggcgc ccggcatatt
    18361 ctgcacgaca atgctcggat tacccggcag atatttgccg agatggcgcg ccagcgtgcg
    18421 gctgtaggta tcgaatccac cgccggcggc ggtgccgacg agcaccacga cgtttttccc
    18481 tttgaagaac tcttgggccg cggccgggat ggcagtgatc aggagaaatg ccgttgcgac
    18541 tatgattttt gcgccgcgcc aaattgcaac atccataaag cgtccttgaa cgtccgtgcg
    18601 gacgttgatg aaagatttta ggagctactt gtctgcaagg tgagcgtcgc tagcatcaag
    18661 cttcacatcg gcgtagccca tcggctctgg tttggtttgg cccaccatga cctgcaagta
    18721 agccggctcg aggccgttgt tttgaaaacc atgaatgact ccggcggggc atgaaacgca
    18781 gtcccaaggt ccgagattct cctctacgcg ccggccatct tcttcctcga agaacaccgt
    18841 cacgttacct tttaggatga aaaacacttc ttcgacggga tgcgtatgcg gtgcattgcc
    18901 ttcgcccggc ggtacgaaca tcacagataa ccgcgtcgca ccggccggaa tatgtcttac
    18961 ctccggcttg ccgagagttt ggccgatgta gcggaactgc gctcgacgat aaccgtcgta
    19021 gttcgagtca ccgaacacgc tccagtccgg cttgatgtcg gcgaatcgaa ccgtgtagcg
    19081 ctctgcaatt tcttcgaggt tcatcttgat gaccgcggga tgacgtgggt gacgagcgat
    19141 gttactcatg cattgcctcc tctagggcgt tggcgaggtt atatcattcg tttttatacg
    19201 cgcactgtag caaaatccgt ttaccgccca agcgcacaac ttgtcaacgc atattcgttg
    19261 caataaaccg catctcccgc aatggcgccg aagcgtaaag tatggaagcc gagtattcac
    19321 cacgaaccac acgaaggaca cgaagttcgg aaaaattggt attgattcgt ccccttactt
    19381 tcgtgatctt cgtgccttcg tggtgagtta ttcgaacttg gcaccttggc ggaagatcat
    19441 cctgtttcag tcgtttcgat tgacagcctg cgctgcttca ccttatcttg cggcttcaag
    19501 taaaaacatt tcttggaaac gttgacatga agtcacggag aaaacttttt tcggtgggca
    19561 cctcggcgcg ccgcgtggac ggcgccccga aggtcaccgg cgcgacccgc tataccgcgg
    19621 accacagttt gccgggtttg atttggggca agtgcctgcg cagcccgctc ccccatgcgc
    19681 gtattcgccg cctcgatatc gaaaaagcga aaaatctgaa aggcgttttc gcggtgctca
    19741 ctgcggccga tcttcccgac cgtttgaccg ggatcattct taaggacatg ccggtgctcg
    19801 ccagagatcg cgtgcgcttt atcggcgaac ggatcgcggt ggtcggcgcg gtgtcgtcgg
    19861 atgttgccga ggaggcgtta agccgcatcg aagtcgacta cgacgagttg ccggcagttt
    19921 acgatccgct gcaagcgatg gcggccgatg cgccgctcat ccacgagcag ttgagaagct
    19981 acgatggact gcggccgccg ctgccggatg tgcctaacct gcataactat gtcgaatggc
    20041 gctcaggcga ttaccggcaa ggatttgccg aagctgactt cgtcttcgag cagagcttca
    20101 ccactcaacg ggcgcaccag ggctacctcg aaccccatgc cgtggttgtg caagtcgagc
    20161 tttccggaaa agtcatgatc tggtccacag ccaagcaggt ttatctcacc cgagccaatc
    20221 tggccgcgtg gctcggcgtc gatgaagaga aagttatctt ccaggtcggc gcggtcggcg
    20281 gtgacttcgg cggcaagggc acgctgatgg acctgccgct ttgctactct ctcgcgaagg
    20341 tcaccggcag accggcgaag atgatcatga gttatgccga agagttgagc gccgctaacc
    20401 cgcgtcacgc ctcggttatc acgatcaaga ccggcgtcaa aaaagatggc cgtctctggg
    20461 ctagagaatt gaaggcggta ttcaacggcg gcgcctatgc cggcatcaag gaaagcagca
    20521 acggcaacct tcccggctcg cgtcatggca acggctccta tgcgattccc cattcgtaca
    20581 ttcagtcgct gtgcgtctac acgaactgca ttcccggcgg catcatgcgc ggtcccggcg
    20641 atttgcaggt caatttcgcc gtcgagtcac acatggatta tgtggcgcgg cggctcggca
    20701 tcgatccgta tcagcttcgt cgcctcaatg ttctcaaacg cggcgacttg ctggttaatg
    20761 gccagcgtgt gaataccgac atgggacagc gcctgctgga aaagatgcgg cgcaaaatcc
    20821 agccgcgtgc gcaggccagg aaaatccctt atgtcggtcg aggtcttgct ttggcttcgc
    20881 gcgatgtcaa tttcggcgcg gcgaatatag atgtcggtgt cagtgaaaat ggcgcggcgt
    20941 acatcctgac aacggttccc gataccggca ccggcgcgca cacgatcttt cgccagatcg
    21001 tcgctgaggc gcttacggtt tcggccgcgg atgtcgcagt tacaatgggc actaccgaga
    21061 tgttcccgac cgatgtcaga gtgattgcca gtctggtcac ctacctgagc gggcaagccg
    21121 tccaacaagc ggccttcgcg ctgcgcgaca agttgattct gcgtgccgcc accgtgctcg
    21181 gctgttcgcc gcacgtagtc cgtttggagc gaggcgccgc cgtcgggccc aagcgcggaa
    21241 aagtcaactt cgccgatctc gccgcccggg caaaatcgaa tgatgaatac ttgagcgctc
    21301 gcggtagcat cgatatcaag gagcgactcg gcgccatgtg cttctttgcc gaggcggcgg
    21361 aagtcaacgt cgatccggat actggacagg ttaaagtttg caaagtgatc tctggccatg
    21421 atgttggaac tatcatcaac ccgctcatgc atcgcggaca ggtcgaaggc ggcatgatcc
    21481 agggttttgg cttcgcgatg agcgaagatc tcaccaccga agagggcaag gtggtaaccg
    21541 cgaacttggg cgagtacaag ctccccacca tccgcgacat accggagcat gaggcgctct
    21601 acattagaga tcacaccggc cccggcccgt tccaagctaa acaaatcggc gagcacggcg
    21661 cgatcccgac cgccgcggcg atcgccaacg cggtctatga cgccatcggc gtacagatca
    21721 ccgatctgcc gattaccgcg gaaaaagtct tcgccgcact gcgccaaggg cccgctgcac
    21781 agcgggctaa tgcggcatcc gtaagatagc ttgccggttc gataatgagg aaactcaaca
    21841 agatagccga aagcggttga gcgtgatagg cccccgcacc gaaatcggca gatcagtaac
    21901 ccgtcttgac gggcaggcga aagtatccgg tgggatgcgt tacgccgcgg atatccaacg
    21961 accaggcatg atctggggaa gatgcttgcg cagcccctac gcccatgcgc gtatccgcag
    22021 catcgatacc gtcgcggcga aagacattga aggcgtctgt gccatcctca ccgcagacga
    22081 cttgccgcgc cgcctggtcg gccgaagatt gaaagacatg ccggtgctgg cgcagcaaag
    22141 ggtgcgcttc atcggcgagc gggtggctgt ggttgcggcg cagactcccg agattgccga
    22201 agaagcgctt agccgtattg tcgtcgatta tgaagaattg aaatcggttc atgacccgat
    22261 tgaagccatg actgccgatg cgccgattgt gcatgagaac ctgcgcagct acgaaattct
    22321 tcatttgccg ctcccggata ttcccaacgt ccattctcac gttcagtcgg tggtgggtga
    22381 cgtcgcccaa ggatttcaag agtccgagct gatttttgaa cagacattca ccacgacgcg
    22441 cgtgcatcac ggctatcttg aaccgcacgc cgtcttggtc gatatcgaag cgaatgggcg
    22501 gatcctcgtc tggtgtccgg ccaaaggtcc ttacgtgacg cgctcccacc ttgcccaatg
    22561 gctcggtttg ccggaagaaa atatctgctt ccagatcagt ccggtgggcg gcgacttcgg
    22621 tggcaaagct tcgctaatgg acattccgct ctgctactcg ctcgccaaga caaccggccg
    22681 tccggtcaag atgatcatga gttacagtga agaattgacc gccgctaacc cgcgccaccc
    22741 atcgctgatc acgatcaaaa ctggcctcaa gcgcgatggc cgactttggg cccgggaagt
    22801 caaagtgatc ttcaacagcg gcgcctatgc tggatttaaa aacagcgaca acgtgaatct
    22861 accgggcgcg cgccacggcc ccggccctta tcatattcct catctcaaga tcgacgcctt
    22921 ctcgatttac accaattgcg ttccgagcgg gatcatgcgc gcgcccggtg aagctcagat
    22981 gctgttcgcg gttgaaagcc acacagattt tcttgccgca caaatgaaaa tggatccgat
    23041 ccaatttcgc tatcgcaatc tgctcaaacg gggcgaccta ttgccctatg gtatgcggct
    23101 ccaagacgat aaggcgcgcc aattgctcaa acggattgcg gccaaaatcc gttggaaccg
    23161 atccaggaag aggcaaccct atgtcgggca agggctggcg ttttgcattc gcgagatcgg
    23221 cgttggcgaa gccaacgtcg aagtgggcct gaaaatcgac ggcggagttt atctggtcac
    23281 cacggttccc gacaccggca ctggcgcgca cacgattttt cgccaaatcg ccgccgagac
    23341 gcttggcgtg gccgccgacg atgtggaaat cgtcatgggc accaccgacc atttcccaac
    23401 cgatgtcgcc gtcgccgcca gccgggtcac ctatctcgcg ggtcaagcca gtcaaaaagc
    23461 cgcgagcggt ttactcgatc tggttctcca ccaagctgca gaaacttttc gctgtccggt
    23521 cgagagactg cggaacggta aaagtattct ccgcaacggc gccaaacaga aaatctcttt
    23581 tgccgagtta gcggcacgcg ccgctgcggc cggacgaaag ctgcaggtca aagccaattt
    23641 taaaattcaa gaacgcggtg gcacagcttg tttcttcgcc caggcggcaa gggtcagagt
    23701 cgatccggaa accggcaaag taaaaattct caaaatgctt tccgctcatg acacgggcac
    23761 ggtgatcaac ccgctcacgt atcaagggca ggtcgaaggc ggcatgatgc aggggttggg
    23821 attcgcgctg atggaaaatc tgcgcgacga ggaaggcaaa atcatcgcgc ccaacctggg
    23881 cgaatacaaa atcccctgcg ccgccgatgt gccgccactt gaaactctgc tgttccaaga
    23941 tcaagacgga cccggaccgt ttcatagcaa accgataggg gaacacagcg ccgtaccaac
    24001 tgccccttgc attgccaacg cgctttacga cgcgatcggc gagcaaataa ccggcttacc
    24061 gctgagcgct gaggcggttt acgatgcgat gaaaaccgtc agggaggcgc ggaaaaccag
    24121 atagctgcag cgagccgatt gcacaagaat tcgaatggcg aagcggaagc tgaagacgtg
    24181 cttccgaaaa ccttcgcgcg ctcctttcat accctgaagt tttagcggaa cgatcgagcg
    24241 ttggcctttg gttgacccgg atttccgtaa acgaggcggt gagttctcga aggtaggaaa
    24301 atgttcgttt ctctgagtgc ggcagctctg ctccgtcaag cccacgtttg atttacgctg
    24361 gcagcagcga ctctggcctt gtagtttcgc cgcaatcctt gctacaagct acggatgccc
    24421 gtttcacctc tgctcgagca accggtaaag aggaagccgc tattttacgg ctgggtcatc
    24481 gtctttgtcg gctttctcgc gcacattgcc tcggcatttt cgatctcgag cacgctggcc
    24541 gtatttctca agccgttgtc cgccgatctt ggcatttcac gcgggacttt ttcactgatt
    24601 cgctcgggcg agattctcat cggcgctgct gcggcgccgg tggtcggtac attgatcgat
    24661 cgcttcggag ggcgatggtt gatggcggcg ggcggtttga tttccggcgc cggcttcatt
    24721 tgcctcggtc aggcaaggga ctttacccaa ttcatgctcg tgcgttggct gcttatcagt
    24781 cccggggatt cattgatggg ctccatggtg gtcaacgttt ccatcgcgcg ctggttcgtg
    24841 cgcatgcgcg gccgcgctct cgcgatcgca gggatggggc atggtctggc caaagtttgt
    24901 atgccgatgg cggcggcaac gctgatgctt tacgtcggct ggcgcggctc ctgggtggtg
    24961 ttcggctttt tcgcgttgct tctggtggtt gcgccggcgc tgctgtttat gcgccgctcc
    25021 cctgaggaga tgggattgct cgctgacggc ggtccggtgg aagtgagaga aaccgccatc
    25081 aaaccaccga agcgtcgcta cgccaagcgc accggcgccg acaatgtcga gtggagccga
    25141 ggcgaagcgc tgcgcacgcc ggtgttttgg ctcatcgcca gcacgttcgg cgtcgcgcag
    25201 atcggcgtca ccggactcaa tctccatgtg ttttcgtatg ttaccgatca aggccatccc
    25261 acgctggtgg cggcatcggt gatgagcatc atcgcggtga tgcagttcag tacgccgata
    25321 gtttggggca tgctcgcgga ccgcatggac aacggcatcc tcaacatggc caagtgcgcg
    25381 ctgcaaggcg ccggcctttt gctggcgctc tcgctcccgg ggatggcgcc gctctatgcc
    25441 ggatttttta tctacggcat cggcatgggc ggcacggcga ttctggcgga aactatctgg
    25501 gccgatttct tcggccgcat ctcgttggga aaaattcgcg gcatgggctc gctcgtgacc
    25561 agcctcttct cggccggcgg cccgccgttc ttcggtttgc tcttcgatta cacggaaagc
    25621 tactacctgt cctttagcat ttttatcgcg atgctgtttg tctccgccgt gctgagcctg
    25681 tgcctgcgac cgccgacaaa aagactggag gtgactgtgt agatagccga ggatggttca
    25741 gaggttcaaa gcttcaaggt ggagctgagc agccttcgac tttcacttct tccgtaactg
    25801 agtctgtcag ccagactcca aattctctag gccgttttag caaactcttc attcacttct
    25861 ttctcatagg cgagcagttt cttgacgctc gcgcgctcct gcatacgctt gaaatgggcg
    25921 gccacgttgg ggaagtctga aatgcttacc cccaactggg ttgcgcgacg gcaacaccag
    25981 aagaaatgcg cgtccggggc ggtgaagtgg tggaagaaat agtcgcgacc ggccagcaaa
    26041 tcgtcaccga tttggaacgc ctcaacgaga aagtccgttg cgatcttgac cacgctgtcg
    26101 tatgcgcccc cgctttcgca gactttggcc ggattgttga tgcggctcag gtagggatgg
    26161 atgccactgg cgcaccagga gtggagcgaa atcgcctgta actcatccca cggatcgctc
    26221 ggcagtatct tagcgtcggg aaaggtttga tggacccatt gatggatggc aacgttctcg
    26281 gtcagtattt tgccgtcgac cacaagcgca ggcaccttgt gtttcgggtt gatcttcagg
    26341 tactcgggcg agaaattctg tcgcttgcgg aaattgagtg gtcgaacccc aaactttgcg
    26401 ccggcttcgg tcagagtgac atagggagcg agcgcgcaag tgtgcggggc atagtagaga
    26461 gcaatttcca ttgatgtctc ctcgttcgtt aaactgatca atgattaacc gcgaatctag
    26521 taccagcgtc tggcaagtgc aatacatcaa aagccaaagc gcttccagta gatccaacgg
    26581 tcccattgtt ttagacgttc aattccggat cgattccggt tacaatcaaa gccttgaccg
    26641 catgccgtat gtgattcaag ctcgcaaata atgtggggcc gacagcgcgt ccgtccggcg
    26701 atgttgctcg cggtttaaat tttctataaa tatctctaga taaccggaca aattcaggag
    26761 gtaggatcat gtcaaccaca accgagcgcc ctctcgctga tgcgctcacc gctatcaaga
    26821 cgcgccgctc agtgaaggaa taccttgaca ccgagattcc tcggcaatgg atcgaagagt
    26881 tgctcgacgc cgcccactgg gcgcccaacc ataaactgac ccatccgtgg cgttttcacg
    26941 tgttcaccgg agacgggcgt gaacgcttgg tcgaagcccg ccaagcggca gtacgtgcgg
    27001 ctgcggaaaa aaagggtgag ccggtgagcg aagacgctct caatttcgct cgcgcaaaat
    27061 gtttttcgtc accggttgtg atcatcgttt ccatgatggg cgatgagaaa aagatcgttg
    27121 accaggagaa ttacgcggcg tgctggtgcg ccatcgagaa cctgctggtc gcggcaaacg
    27181 cgcggggcct gggcagctat ccgagcaccg gtgactggat cgaccaaaac ttcgtcggcc
    27241 cggttcttgg actcacggaa aaagagcggc cggtggcgtg catttttctc ggctattccg
    27301 aacagaagac gatggccaag cgcctccccg tggagcgcca tacgacgtgg tacaccgagc
    27361 cctgaaattc ccgcctgaat agagactccg agatcgagac tctttcaatt ctagtgcgag
    27421 gccggtctgt tccgagaccg gcctcgcacg ctaatttctg cgctcgccaa gagtgcatgc
    27481 catgacaagt agatagcaac tcctaatccg acacgcgcgt cctctcccac gcctggcggg
    27541 cattgattga tccagtcatg gtcgagacga agtgacaacg aggtttattt agcggcgaac
    27601 gccgagaaaa cgagcacgca aagtgccccg tgcctgtcag acggcgttgc gaccgacttc
    27661 cctcaggcat tcgggcattc ggcgtcggta acatttagcg tgatttggag ttgtcgtgcg
    27721 tggtgccccg gcgcaagtag cgataagcct cagagacgga gttcaaagat cacgctggta
    27781 tcgcttagct ccggactctg acccttggtt ccgagcattt cgacagactc agccgatcac
    27841 ttccgagcga catttccata tagttcgtct cggcacgttt caacgcggtg acggcagagt
    27901 gccgtggttc gaaccgttga cttcgcgatc agctgtgtgc gatggggtat tggcgatcag
    27961 agaaccgtcg gaggtgatgc atgcgttatc gttctcgacc ttggacactc tgtctctcga
    28021 ttttcgtgat tctctgttgc tgcggtctcg ggcatgtctc cgccaacgat gtcgtcctca
    28081 tgtcttatgg tggacacaac gaaactatcg gaccgtactg ggttgctgtc gataaagggc
    28141 tttatcagaa gcacggtatc gacgggcgct tgctgcaagt gcgtagcgcg cagatcagtt
    28201 tgtcggccct catatcgggc gaagtcccga tatttcttcc ctccattgca aacgtgttga
    28261 gcggtgtatc cgccggcgcg aagctcggct gcattgcttt tccgatcaaa ggcgtttcac
    28321 gagaattggt ggtgcgccgg gacatcgatt cgctggcagc gctgcgcggc aagaccatcg
    28381 gcgtgcaaag tatcggtggc gggttttggc tgcaaaccat gatgttgctc gacgcagccg
    28441 gcgtagaccc ggacaagatc ggcatgaaaa cgcgcgtgat tggcgaggag gcgacaatcg
    28501 tgcaggcttt ggtgagcggc aacgtcgatg cggcagtcgt gactttgccc tccgcgcagg
    28561 tggccaagcg tggtggcgcg cgcactttag tcaccacggc ggattttcct atcgtattgc
    28621 aaactgtcgg cttatgcggc cgcaacgaac gtatcgcgaa ctcgcctgaa ctgatgatgc
    28681 ggctggccaa aggtatggtt gacggtatcg tttatattct tgatccgcga aacaaaatag
    28741 aggttagcgc gattttgaag aagcatttac ggctgagcgc cgatgagatc gcggaaacct
    28801 cttatcattc gctcagaacg ataattagtc tcgatatcgc gccggacgtt gccgcgttta
    28861 aaaacgtgca acgaattgcc gcgcgggtca atcctaaaat cgcccaagtc gacgtcaacg
    28921 aagcgatcaa cctgaacgtt gtgaaggctc tcgaaaatag cggctactta gccgaggcaa
    28981 gaagaaaggt tgcgcttacc cgctagtgtc ctgaccccta agttccggga taaatcagca
    29041 ggtctcatag cagcagccca aaggcatagt ccgaatagac taattagcta gcctgcacaa
    29101 gtagtactca cttctgtgcc tatcgttttg cttgatcgca gaatggacaa gttcgactac
    29161 aagctttgct tgagcccggg caagatctta atccctgaag aacggttttc aaccgacgaa
    29221 gacacatccg gatgtttgta atgaccggag aatatagtga acacggattt agggcggccg
    29281 ttttggagat tgcaaaagcg gaagcaaaag ccatgttgac ggaatgcggg cgcgacaaaa
    29341 agatgattta cgtacacgga ttttctatat cagatccgct cgatccagtt gcaccgttcc
    29401 tttggcctcg agcaaaaaga ttcccgcttg tctagtgctt caatccatag aacctagcgg
    29461 cattcccata gaggatcgcg tccttcgccg gcgcgctgat ctcttcgttc tcgcgcaact
    29521 cgcggatctc tttctgcacg gtgtgcgtgt tcacttcgtg aggaaagtcc gaggaaaata
    29581 tgaacgctcc ttcgccggcg accttgatcg cgtagggcag gtccgattcg ccaccttcga
    29641 tcccaaccac cagccggccg tcttgaacga gcttgcggac gtagtccccg acggtttggc
    29701 cctgaggcaa ttgcagcaac tcgccgcgcg ggttgatcgg cacgcccgct tcgaacgagc
    29761 gagtaagccg cgcgatcgcc atcagaaacc acagaggtcc gccttcgagg taagcaaccc
    29821 gcagtcccct gtagcgctcg aataaattgt tgagcagcat ctccgtcaag gcgatcgcca
    29881 gagacatcgg atgtccaatg gcattggcgc cggtggaaat attcatcgag tccatcccca
    29941 gatcccaatg gccgccgcca tgaacgacga cgggacagcc cagtctggcc gcttcttggt
    30001 aaaccggcca atagggtttg gcgccgagat tcaattgcac tgccgtggcc gggaataaga
    30061 ctccggaaaa ccccaatttc gtataggccc ggttcaactc ctccacagcg gcctcggcat
    30121 cgtgcatggg aatgagagcg atacctttaa accggggatc gcgttgcaga taggcttcgg
    30181 ccagccagtc gttataggcg cgcgccgcgc cgaaagcgta gtcgcggtcg acgatgcggc
    30241 caatgcgctg gccgtgtgtc gggaagagaa cagtatattc gaacccggtc tgatcgagaa
    30301 attgcagcca gccttcaggc cccgggttgt gaaactgacc atcggcgccg gccccgaacg
    30361 ccccgggcgg agttcgggtg agagaatact gcatgtggct attggcgggg aacaagccat
    30421 tgcggaacgt gatccggccg gtttcaacgc cgtaaggaaa atgccgggca atggcctggt
    30481 tgtcctcgaa gacatggccg tcgccgtcga tgattttcga ttgatctttc ataggagctc
    30541 tcctccttgc caacaaacga ttgctgtggt cgtttataac tcaatcagca atccattatc
    30601 atagtcttat cccggcagta atcgcgtggt gcaaacaact gaaggatcta gggtgaagta
    30661 ttatccctaa tattcggcat cttctagggc ggctcttgaa gctgactgat catcattccg
    30721 ttcttatatc atacctccag atccgtcggt catcagatta ctttgaagtt gtctccttca
    30781 acatccgttc gatatccggt ttaaaggatt ccagaggctg cgcgccggtt ttcactacgg
    30841 ctgtgccggt ttcgtgatga aaaagaatgt tcgccggcgt tccagtgatc ccgatcttag
    30901 cgccttcatc taaatcttcc ttcactcggg ccgcgtactt actgctttcc agacagcgcg
    30961 tgaagcgatt ttcatcaaga ccgatttctt tagccagcgg gaccagctcg ctcaacggaa
    31021 accccttgcc gttcgaggtg gttcgttcat agatcgaatc ggtgtatttc cagaacgctt
    31081 cgttacctcc gaactcatta gcacattcag cggcttcagc ttgtttctga gcgcccggat
    31141 tgtgaaaact caaaggaaag tgccggtaca cccaattgac tttgccgccg taggctttca
    31201 cgatcgcctt tgctgtggga tgaaaacttt tgcagaaggg gcactcgaag tcagaatatt
    31261 cgatcaatga aatcggcgca ttcgaattac cataaatatg atctcgtgta accgacacgg
    31321 gacgaacatt ctgggccatc tcattggcga tacggacttg tttgagtttt gcggcaacct
    31381 gagcctcttg ctgtttcttt acatactcct gaatcccgag ctgaatctgc tctttgagaa
    31441 agtcgctttc gcgcagttcc ttcaggacct cttctttggc cttctgaata atacgctctt
    31501 catcaggtga tagacccttt cccggttgag tctgcgcatg aacttggaac agattcgtat
    31561 ccgaccaaac cagtacggct gtcaaaagta acgcgctcca gtaagttttt tcaatcactg
    31621 ttcctcctaa ggagccttca ttttgttcaa tcaatggttt gtcggcttgt gtctttagct
    31681 agttacgctt ctgcgattaa cggacttttt ctcgtttttg gttggagaat gatatagcat
    31741 acggtcatcg ttaaatccct gtttcggctc gtgcaacaaa cccgcttgtg tacgggcaga
    31801 attcggatga tgcagcgcca ctacctgttg ttgcgcaagc gcaaacactc cgcatcaaga
    31861 gtgaggtctc caattagcct tatgtcaatg tgaagtgtgc ccaactatgc gaccggcgac
    31921 aacgaaagaa gccgaatgac gacgaactac gaccaaattg cggcggaata caaacgcgcc
    31981 aagcaacaac catggcggct gcatcttgag cattacacca tgttcaagtt actcggcgat
    32041 ctaagcggca agacagtgct cgatctggcg tgcggcgaag gcttctacac acgattcatt
    32101 aagaggcaaa acgccgcgcg cgtggtcggc gtcgatcgct ccgccgggat gatcgcgctg
    32161 gcgaacgagg aagaggagcg ccatagcctg ggcatcgagt atctggtgca ggatgtcaag
    32221 cagctcgcgc tcgatgaaac cttcgatatc gttacttccg gctatctgct caattatgca
    32281 gagagtgctg ccgagttgtc ggccatgtgt gcggccatta cccgccatct caaaccgggg
    32341 tgcaggtttg tcaccgtaaa caacaatcca cggcatgagc ccgagtactt tgcggcgacg
    32401 cgcaaatacg gcttcgtaaa aaaagcggag cgctcgctga ccgaaggacc gccggtggat
    32461 tacgtgttct atctcgacga gggttcgttc gcgatcacca attatcactt gagcatcgaa
    32521 acgcatgaac tggcgttgaa gagcgcaggg tttcgtcagg tgacgtggca cgacccgaaa
    32581 ttatccgccg atgtcgcaga tcctagcgcg cgacagtact gggccgagtt tcttgcgcat
    32641 ccaccggttg tgttcatcga ctgtatcaaa taaacccatc gacggaatgt tcaggagcat
    32701 gatcgtatgg aatccggcca atcgccgagg ggcttgtcga tacgaaatcg gaaggtccga
    32761 tgttttcgca ccctgcggtt cttagatcta tgccgttata gcgatgtatc atcgtgctgt
    32821 cagcagctct atatctgata ctcaaatgag aacgcaaaaa agttgactcg cgtcattgca
    32881 atattatcaa cggtcaacac gccaatgacg acaagttttg agacgaagcc aaaactaggt
    32941 atcagcgcct gtctgctggg acagaaggtc cgcttcgacg gcggccacaa gcgcgagcaa
    33001 tttctaaccg atctgtttgg caggttcgtt gactggattc cgatctgccc tgagatcgaa
    33061 gccggcatgg gtgtgccgcg cgaaagcgtt cgtctggtgg gcacgtctgc tgatccgaac
    33121 atgattgccg agaaatcagg cagagattgg acggcggcaa tgaatcagtt cgcggccaag
    33181 cggctgcgcg ggctcgctgc agccaatttg tcgggctacg tgttcaagaa gaattcacct
    33241 agctgcggta tggagcgtgt aaaagtttat agctccaaag gcatgccgga gcggcaaggg
    33301 agaggtcttt ttgccgccgc ggtcatgaag cattggccat ttttaccggt cgaagaagaa
    33361 ggccgcttaa acgatctaaa gctccgagag aacttcatcg aaagagtttt cgcttatcat
    33421 cgctggcaac aagcaatagc cgggcgaaaa tctttggcag cgctggtccg gtttcatacg
    33481 agtcataaat atttgttgct ggcgcacagt caacctcatt atcgccgact gggcaggctg
    33541 gtggcgacgg cgaaacaaaa atctcttggc gcggtttacg acgcgtacgg acggatgttt
    33601 atgcaggcct tggcggttca tgcaaccgag aagactcatg ccaatgtttt ggaccatcta
    33661 atgggctatt tctccaagca attgaccgca gccgagagag aagagctggt gcaattaatc
    33721 agcgatttcc gccaccagct cattcccttg atcgcgccga tcacgatgat tcggcactat
    33781 acgaagaaat atcgggtcga gtacttgctg ggtcaagttt acttggaacc gaatccgaaa
    33841 gagctgatgc tgcgaaatca tgtatagcac gagactttcc gttacgttga tagtttgatt
    33901 ccaagagaat aacgtaatca acggcggcca gcttcgacgg agcgttcggt ttcgaagcat
    33961 gccggatctg tcagtcaaga aaaagttagc ttgtctgctg tttttctatc ctcggtcgag
    34021 cctttaactc cgtagcatga tatgtatggc tggatgtgga atatccggcc agttggacgg
    34081 cgtctttttt tcgtttttca tcgagcaagc tatcatcgga gctgccatcc tgccgttaat
    34141 tattcccggc gtcagctgag ccgggattaa ctacgaacaa aatatccccg tgtagtaaac
    34201 ttggagccag cttgagcgac atccctggta ttgagagatc cggaaaagaa cggcggcgaa
    34261 ttttttttgg ctggtatatg gttgcggcaa gtgttgcggc caacacgatc ttttccgccg
    34321 cctacttcca gggctttggc gttctcatcc tgccgattga gcgcaccttc ggctgggacc
    34381 gttgggttat ttccgcggcg atgtcgctgc ggcagctgga gtctggcatc gtcagtcccg
    34441 ctgtcggatt cctgctggat cgcttcagcg cccgtaaatt gattttctgg agcgccgtca
    34501 tctcaggcac cggcttcatc ggcctcggct ttaccagcgg catcgtcatg ttttttctct
    34561 gcttcgtgat cgtctccctg ggagcgtccg gcgtcagcca tgcggtgacc tggccggtga
    34621 tcatctcacg ctggttccgt cgcaaccgcg gcctcgctac cgggctcgcc gtaaccggtc
    34681 caatcttcgg ctcccccatc gttatactga acacgcagat cgaagaggca tacggctggc
    34741 ggatcgtgct gttcggctac ggcatattga tcctggtcgg catcacgttg ctcagcatgc
    34801 tggtgcgcga ccgccccgag ccttatggct tgcggcctga tggtgatccg ccggaggata
    34861 gcgccgccac ggaagatctt aatgttccat cgcgccgccg aatcgatgcg ggactgacgc
    34921 tgcacgcagt tttccgcacc aaggaattct ggctattcac gagctatttg tccggtacgt
    34981 ttgcggtaaa ctccgccgtt caaggccaca tgatccccta cttccagcaa gacatcggac
    35041 ttagcgctgc ttgggcggct gtggtgatgt cgatggtctt catcatcagc ggtatcgggc
    35101 gcatcggcgg tggctatctg ctggacagga tggactatcg cttagtcctg gcagtcgttg
    35161 ccgcaatgat gggtcttggc ctgttgtacc ttcaggtcgt gccggtcaaa tccgtttggg
    35221 ccaccttgcc attcgcggtg atgttcggcg tcagcttcgg ctgcctcgtt ccgatgcgcg
    35281 gcaccgtcgg cagcatcatg ttcggcaccc ggaccatcgg agggatcttg ggactattgc
    35341 agggagggcc gattgcggcg ggagttatcg gccccctggt catggggctg atcttcgact
    35401 tgcacggacg ctactcgacc gcgatttggg gactcatcgt catcagcgct ttgatgatac
    35461 cgctgtccct ggctatgggc tcgccggccg agctcgccaa gcgcattcgg gcagctgaca
    35521 gcgatacgcg cgttgaccta cactgaaaca ttgggcgaga tgtcagtcac tccagacgct
    35581 cgcgcacatt tcatcatgtg ttgcaggaat tttcgtacca cagcagcaca cagagtcgcg
    35641 ctttgattct cgtttagatc acggcttagg gctttgtatt actggttggc ggcggcgcgg
    35701 aatttgttgt tctttagcaa gttgtaattg tcgatgagct gtttcttctc ggcttccgaa
    35761 atgccgagct gcgggcgtcg cacgaagctt gtcttgatca gtccttcgtg tgttaacacg
    35821 gccttgatgc gagccacagc ttcgacgatt ggttcgctga aaaccagctc tttcaccgat
    35881 tccaattcac tctggatggc ggcggcttga tcgaagtttc cggcgcgtcc ggccaaatcc
    35941 aacgcgcccc atttttcggt cgcgacgttg gccacaccga tgagggcgcc gtcggcgccc
    36001 attttgtaac tctcggcgac gaatcggtcg ttaccggtca acactcgcat ctgggcgttg
    36061 tcgcgctcca acgctccgat tgtgtcgcgg aacagattga tgtcgaaaga cgcttctttg
    36121 aaggcgacga cgttttccag ccgtgcgatg cggcaaatgg ttgccggatc gtaatagtag
    36181 ctcttaaccg gtatctgaaa aagcacgatg gggagcttgg tttcccgctc caagtcctcg
    36241 aagaagcgca ctttgaggtt cgggtcgacg ttgccccagg caaacagcag aggcggaaag
    36301 atcattacac catcgaccgg gatatgctcg agatcgcgca ggagtgcaac cgcttcatcc
    36361 gtcgactgcg gcgcaattgt ggcaatgtgc actttgccgg cttcatgcgc aaccttgccg
    36421 gaaagttcgt aaacgcggcg cttttcctcg atgctcagca ccgggctttc accgatacgc
    36481 gcgcaggaga ccaatccggc aacgccgggg cgcgaagcaa aataagcact cagtcgagtc
    36541 aaactttcga tatcgatagc tccggattga tcgaacggag ttacatgggg gatgaagaga
    36601 ccgctgaagc gttcatgaag cgatggcata gagccctccg gccgttttgt gctaagactc
    36661 ggtagataat atcgtgcgta aaagactcgg cataagtaga acagattgcc cggacaggtc
    36721 aagcaaagga cttttcattc ctacgagatt ctgaccggca tcgggcttac tctcagcgaa
    36781 ttgaggagct taagatgaac gcgcgtattt cgtgcgccga gcttgcccgg ctgatggagt
    36841 ccaacgagcg ttttgcggtt ttcgacgtgc gcgaacgcgg tgaatacaac caatgtcaaa
    36901 ttcctaacgc aacttcactg ccgcgaagcc aaatcgaatt ccgtatcgct cagttggttc
    36961 ccgatctcaa ggttcggttg attatttacg acgagggcgg ggcgcgggcg ccgttggcct
    37021 tgtcgacatt ggtgcggctc ggttacgaac atgttgccat cctcgacggt ggtttgagcg
    37081 catggcaaag ccacggacgg ctcacatcca gcggtgtcaa tgtgccgagc aaagcttttg
    37141 gcgagcgagt tcatcatgag agaaatgttc ccgacttggc gccggaagag ctcaagtcgc
    37201 tgcaggatcg ttcgacagat ctgattatcc tcgatgttcg cactccggaa gagtacgccc
    37261 gcttttgcat tccgggtggc atcaacgttc ctggcggtga tctgattctc tgggccgaag
    37321 aattaaagca gcgtaagggg acaaatgtca tcgtcaactg cgccggccgc acacgcagca
    37381 tcattggggc cgcggcgttg cgccggctcg gcgtgggtaa cgtccgggcg ctgagaaatg
    37441 gaaccatggg ttgggtgctt gccggttttg agctggaaga taagcccgaa cggacgccgc
    37501 cgcccgcgcc ggccacagat gctgaag
//
LOCUS       DASUVS010000383         7242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1914632, whole genome shotgun sequence.
ACCESSION   DASUVS010000383 DASUVS010000000
VERSION     DASUVS010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914632"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="VF182_20535"
     CDS             complement(<1..1125)
                     /locus_tag="VF182_20535"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX6439515.1"
                     /translation="MAQTDASFNIIETTIEDVHAAYKSGRLTCRQLIQMYLDRIEAFD
                     KKGPQINAIITINPEALPEADRLDAAYKASGPVGPLHGIPVIFKDQGDVKGMPTTLGS
                     LLFKDYYPDRDSFVAEKLKKAGAVILAKATLGELGGGDTHGSLFGSTRNPYDTERTVG
                     GSSGGSAAAVAANYSTLGVGQEGLASIRRPSTWNCITGMRPTAGLVSRGGVYGCWPEI
                     NGSLGPMARTVGDLAALLDIMVGYDPEDPITARGIGHIPESFKSFLDTNGLKGARIGI
                     LRESIGLGSEPDSEDFKKIGEVFDKAVADLEANGAEIIDPITIPKLKELMAKRSSGPG
                     DSDKSFQNYYGRSARPPFTSRAEVIAAPEFAKVARRSQDRF"
     gene            1518..2684
                     /locus_tag="VF182_20540"
     CDS             1518..2684
                     /locus_tag="VF182_20540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6439516.1"
                     /translation="MARRVAGFGTTVFVEINELARQCNAVNLGQGAPDFDGPPEVLAA
                     AVEAINGQLNQYAPGVGMPVLRQAVARHAERFYGQRVDWQTEVLITSGATEAVFAAIL
                     GLTDPGDEVIVFEPVYDSYVPNLVMAGVKPRYVPLRGDHWSFDADELAKAFNQRTRAI
                     IVNTPHNPTGKVYSREELSLIADLSRKHRILAITDEVYEHILYDGIAHTRLATLPGMA
                     ERTVTISSVGKTFSVTGWKIGWAIGPADLVNAVNRAHQFITFAVASPLQAAAAAALSL
                     PASYFEDMQMSYQAKRNGMLRVLEQAGFRVLMPQGSYFIMADWRAVAPPHVTDDVQFA
                     RWLTTEIGVACIPPSAFYQESDKALGRYLARFAICKKAETLAAAAERLQRLGKL"
     gene            2911..3288
                     /locus_tag="VF182_20545"
     CDS             2911..3288
                     /locus_tag="VF182_20545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HEX6439517.1"
                     /translation="MKTKGELEAAVSEAMVGFEIDCLGRGPKESRSHIIEDMILVRLK
                     GMLTPAEQQLAKRADGVELIKKMRSTLIESARDKLATIISDVSGAKVLDILTDMNTAS
                     GERVFVFILDANLGRDLPRKISA"
     gene            3539..4390
                     /locus_tag="VF182_20550"
     CDS             3539..4390
                     /locus_tag="VF182_20550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6439518.1"
                     /translation="MADQNPAAEEVLLDIRVAEASGAYKFYLNNEKKILGTTAVIVFL
                     TVWELMGNTLQLINPMFMSAPSLIWKAAVQLFTSGEIWNDLRVSGIEFAWGYVLSVLV
                     GVPFGIACGWYKKMAYIFDPFVNAMNATPRVALLPLVIIWLGIGILSKVGIIFLGAVF
                     PILINARDGVKTTPYNLLTAAKSFGASEWQIFRSVVLPSTLPFILTGLRLGVGRALIG
                     VMVGELYAATAGIGFMITVAGATFQTDKVFVGVLIFAITGMVAMELLTKLEGRFEKWR
                     PKVGAAA"
     gene            4820..6478
                     /locus_tag="VF182_20555"
     CDS             4820..6478
                     /locus_tag="VF182_20555"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HEX6439519.1"
                     /translation="MAKNIVICSDGTGNSAVKDRGTNVFKIFESIDLNGHRLNPKLDP
                     QVPIYDDGVGTEDFRPVKIFAGITGWGLSRNVKQLYKELCHIYDPGDKIFLFGFSRGA
                     FTVRTLAGFIIKCGIIDAQKQPTAKAFDECVGRAYKAYRSCYRTALMRLLRGEANAAA
                     TDEFRGQYCHAQQTRIHFIGVWDTVDAVGMPFHVSDLINALIYRFKFPNQRLSEQVDH
                     AYHALAIDDDRHSFHPLLWDQRDSSPGQSQKIEQVWFPGAHANVGGGYPKQGLSLIAI
                     HWLMTKAQNAGLRFIPGEYEYYRDHANVDDKLYDPRAGLGVFYRWKPRDIAQLCRDNG
                     VTPAMHLSVLERIAHGTEDYSPGNLPPEARIVITPYDDREKDATAMTRALAAEAILKS
                     AHANTQGSLLNCVRGTINVGRLSYYIYLLSCITALIAASGALDQPSGTGIWPITKGLI
                     LFLVNLVASPFATLYAVGQNFSRDLIRIAWIASGFLLAYLLMKITDKRMSAVFSKFWF
                     EQQKNLRVALKQARQEANDIISAGLSSPQHPTAHIDKEPRIRVS"
     gene            complement(6535..>7242)
                     /locus_tag="VF182_20560"
     CDS             complement(6535..>7242)
                     /locus_tag="VF182_20560"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6439520.1"
                     /translation="EVQFDVRKFGWIGSPASESVVLYMSKDAPYQSIRDIIKAKEPPK
                     CGSSGTVSTDYLLARLLEDSIGAKFNTVIGYPGGSEIDLAVEKNEVVCRAHNISAHFG
                     REPFNSWHKKDFDRHIIQTGRKRDARLPDTPTIYELFDEYKSPEASRRVAQVILTGGD
                     LGRPMLVTPGTPPERVKTLREAYVKALKDPELLVETEKAKMDVDPTPGEELEELINKV
                     MDQPKEVVDRVKKMLGN"
BASE COUNT         1590 a         1965 c         1969 g         1718 t
ORIGIN      
        1 gaaacgatcc tgcgatcttc gcgcgacttt ggcgaattcc ggcgccgcga tgacctctgc
       61 acgggatgtg aaaggcggcc tggcgctccg accgtagtag ttctggaaag acttgtccga
      121 gtcaccggga ccggaagagc gcttggccat cagttccttg agcttgggaa tcgtaatcgg
      181 atctataatt tcggcgccgt tggcttcgag atcggccacg gctttatcga acacttcgcc
      241 gatttttttg aagtcttcag aatccggttc actgccgaga ccgatcgatt cgcgcaagat
      301 gccgatccgc gcccctttaa gaccatttgt gtcgagaaaa cttttaaagc tttcgggaat
      361 atggccaatc ccgcgcgcgg tgatcggatc ctccggatca tatccgacca tgatgtccag
      421 cagagcagcc aaatcgccca ccgtgcgagc catgggaccg agcgagccat tgatctcggg
      481 ccagcaacca taaactccgc ctcggctgac cagacctgcc gtcggtcgca tgccagtaat
      541 gcagttccac gtcgacggcc tgcggatcga tgccagcccc tcctgtccaa cgccgagggt
      601 cgaatagttt gccgcgaccg ccgccgcgga cccgccggaa gaaccgccga ccgtgcgttc
      661 tgtgtcatac ggattgcggg tcgagccgaa cagcgatcca tgcgtgtcgc cgccgcccaa
      721 ctcgcccaac gttgctttgg ccaggatcac cgcgccggcc ttttttaatt tttccgcaac
      781 gaacgaatcc cggtccggat agtaatcttt gaacagcaag gatcccaatg tggtcggcat
      841 gcctttgacg tcgccttgat ccttgaagat caccggaata ccgtgcagcg gtcccacggg
      901 acccgaggct ttgtatgcgg cgtcgagacg atcggcttcc ggcaaggcct cgggattgat
      961 cgtaatgatc gcattgatct gcgggccctt tttgtcgaaa gcttcgatac gatcgagata
     1021 catctgtatt agctgccggc aagtcagccg gcctgacttg taagccgcgt gaacatcttc
     1081 aatggtcgtt tcaatgatat taaaagacgc atcagtttga gccatcgcgt aatctccttt
     1141 tcgttttcag ctagccgatc gaaccttggt tattcaaacc tgatgacaat gtccagagtt
     1201 cttgctcgct gccgattgat accgacagga acggacacca tagcattgac tctgtacaca
     1261 aacatatagc gtcacccccg aatgttttaa atcgggggtc catttccgct ctcgacagga
     1321 ttcccgatac aaacattcgg gaatgacgga ctaggagggc taggaataaa gaatagataa
     1381 ctcagcaagt gcggtgagga cgctagcata ggcgtatggg atcctgaaca gaacctcgat
     1441 gccacaccat tgcaatctac ctggttaatg attattccga agagtctctt gaaagggtgc
     1501 atcacacgat gtcagctatg gcccggcgcg tcgccggatt cggcaccacg gtgttcgtcg
     1561 agatcaatga attggcgcga caatgcaatg cggtcaatct cgggcagggc gcgccggact
     1621 ttgatggtcc gcccgaggtt ctggcggcgg cggtggaagc gattaacggc caattgaacc
     1681 aatatgcgcc gggtgtcggc atgccggttt tgcgtcaagc ggtggcgcgc catgccgagc
     1741 gtttttacgg ccaaagggtc gattggcaaa cggaagttct gatcacctcc ggagcgaccg
     1801 aagctgtttt cgctgcgata ctgggcctga cggatccggg cgacgaagtg attgtctttg
     1861 agcccgtcta tgacagctat gtgccgaacc ttgtcatggc gggagttaag ccgcgctatg
     1921 tgccgttgcg cggcgatcac tggagcttcg atgcggacga gcttgcaaaa gctttcaatc
     1981 aacgtacccg cgcgatcatc gtcaacacgc cgcacaatcc cacgggtaaa gtttattcac
     2041 gcgaggaact tagcttgatt gcggatctct cccggaagca tcgtatcctc gccatcaccg
     2101 acgaggtcta tgagcatatc ctctacgacg gaatcgccca taccagactg gcgacgttgc
     2161 ccggcatggc agaacgcaca gtgaccatca gcagtgttgg caaaaccttt agtgttaccg
     2221 gatggaagat tggctgggcg atcggtccgg cagatttggt gaatgctgtt aatcgggcac
     2281 accagttcat cacctttgca gttgcctcgc cgttgcaggc tgcggcggca gcggcactca
     2341 gtttgccggc cagttatttt gaagatatgc agatgagcta tcaggccaag cggaatggaa
     2401 tgctccgtgt attagaacaa gccggtttta gagttctcat gccgcagggc agctatttca
     2461 ttatggcgga ttggcgcgcg gtagcgccgc cgcatgtaac cgatgacgtt cagttcgcgc
     2521 gctggttgac gaccgaaatc ggtgtcgcgt gtatcccgcc cagcgccttc tatcaagaat
     2581 ccgataaagc cttgggccga tacctggcgc gttttgccat atgcaagaaa gctgaaacgc
     2641 tggctgccgc agccgagaga ttacaaaggc tcggaaagct ttagggcgat tcagtcatgc
     2701 tggcctttca aaccgtgcgc ccccgttgtt ggtccctctt agtcacaagg attcagcttt
     2761 cgacgcccgc gcggccaaaa gatacatctc gaggcactag gtccaaagac cgattgacgc
     2821 acctagctgc atctggctaa gtttagttcg catcggcagc ttgttacgcg ctgagtatca
     2881 aatggctcgg gacgatggac cgcatcttct atgaagacaa agggcgaact agaggcagca
     2941 gtcagcgaag ccatggttgg attcgaaatc gactgtttgg gccgcggtcc gaaagagtcg
     3001 cgtagtcata tcatcgagga catgatactg gtcaggctca agggaatgct gacgcctgcg
     3061 gagcaacagc tggccaaacg cgccgacggc gtggaactga tcaagaaaat gcgttccacc
     3121 ttgatcgaga gcgcgcgaga taagctcgca accatcatca gcgacgtcag cggcgccaag
     3181 gtcctcgata tcttaacgga tatgaatacg gcctccggcg agcgtgtttt tgtctttatt
     3241 ttagacgcta atctgggtag ggatttaccg cgtaaaatta gcgcttgaca attatactgt
     3301 tcgttattag cgtagctcta gttttgcaga tgactcgagg ggctgctccc gcacacggat
     3361 cgcagccagt tgggttgtaa gccgacttga gatgtcaaac atctggtcgg cttttttgtt
     3421 tttactggat tagacaggcc agttggagga agccctatga gggcgtctcg attcaaggca
     3481 cgccagtcta cccgattacg gagggctggc ttttttattc gtaaaaggag ggaaacccat
     3541 ggctgatcag aatcccgcgg cagaagaggt cttactcgac attcgcgtag cagaggcttc
     3601 cggagcttac aagttctatc tcaacaacga gaaaaaaatt ctcgggacta cggccgtcat
     3661 tgtattcctc acagtatggg aactgatggg gaatacattg cagttaatca atcccatgtt
     3721 catgagcgcg ccttcgctca tctggaaagc cgccgtgcaa ctgtttacat caggagagat
     3781 ctggaacgat ctgcgggtta gcggtatcga gttcgcctgg ggatatgtgc tctccgtgct
     3841 cgttggcgtc ccctttggga tagcctgcgg ttggtacaaa aagatggcct acatcttcga
     3901 tccgtttgtt aatgccatga acgccacccc gcgtgtcgcc ctgctcccgc tggtcattat
     3961 ctggcttgga atcggcatcc tttcaaaggt cgggattatt tttctcggcg cggtgtttcc
     4021 gattctgatc aacgcgcgag acggcgtaaa gaccactccc tataacctgc ttaccgccgc
     4081 aaaaagcttc ggcgcttccg aatggcagat attcagaagc gtagttctgc cgtcgacttt
     4141 gccattcatt cttacgggtt tgaggcttgg cgtgggcagg gcattgatcg gtgtcatggt
     4201 tggcgagctt tatgctgcta cggctggcat cggcttcatg atcaccgtcg cgggcgctac
     4261 tttccagacc gataaagtct ttgtcggcgt tctgatcttt gcgattaccg gaatggtcgc
     4321 aatggagctc ctaaccaagc tggagggtag gttcgagaag tggcgaccga aggtgggcgc
     4381 tgccgcgtag ttgctgctac atataagtta gatcgtagag atcacggaca ttaacaaagc
     4441 cgattacccg taggtaatcg gctttgtttt tgggtcattt gcctgaatat gtaagcctgg
     4501 tcgtaaaccg gctttgtgtc gaggactcca gatgggtctg acgattcctc ggacacccgc
     4561 ctctcggaca ctcctttggc cgctggtgac agccatgtag atagctctcc tcatcaagcc
     4621 cagaataatc tggctccgga cggattttga ccggagtatc ggatcggtag gaagtctggc
     4681 catctccccc tgcatgaaaa tagatcggat ttcctcagca atgaattttg ttgaccctgt
     4741 tgtattcagt cgattactct taacctgctg aacgtcgccc gagatttttc ctaccaagcg
     4801 tttattctac ggagaaccca tggccaagaa cattgtgatc tgctccgacg gaaccggaaa
     4861 ctccgcagtt aaggaccgcg gcacaaatgt gttcaaaatc tttgagtcca tcgatctgaa
     4921 tggccaccgg ctgaacccga agctggatcc tcaggtgccg atctacgacg atggcgtcgg
     4981 cacggaagat tttagacctg tcaagatttt cgccgggata accgggtggg gtttgagccg
     5041 gaatgtcaaa cagctgtata aggagctatg ccatatctat gatcccggtg acaaaatatt
     5101 tttgttcggc tttagccggg gagctttcac ggtccgtacc cttgcagggt tcatcatcaa
     5161 atgcggcatt atcgatgcac agaagcaacc taccgcaaag gctttcgatg agtgtgtcgg
     5221 tcgagcctac aaagcttatc gcagttgtta ccgaacggct ttgatgcggc tgctgcgcgg
     5281 cgaggccaac gctgccgcga cggacgaatt tagaggtcaa tactgccatg cgcagcaaac
     5341 caggattcat ttcatcggcg tttgggatac cgtcgatgcc gtcggaatgc ctttccacgt
     5401 cagcgatctg atcaatgcgt tgatctatcg attcaagttt ccgaatcaaa gattgagcga
     5461 acaggtagac cacgcctacc atgccctagc gatcgacgac gaccggcatt cctttcatcc
     5521 tctgctctgg gatcagaggg attccagccc cggccagtcg cagaagatcg agcaggtatg
     5581 gtttcccggc gcccacgcca atgttggcgg cggctatcca aagcaaggct tgtcgcttat
     5641 cgcaatacat tggttgatga ccaaggccca aaatgcgggt ctcagattta ttcctggcga
     5701 gtacgaatac tatcgagatc acgccaacgt cgacgataaa ctctatgacc cgcgcgccgg
     5761 cttaggcgtt ttctatcgct ggaagccgcg cgacatcgcg cagctctgcc gtgataatgg
     5821 agtgaccccg gcaatgcacc tgagcgtctt ggaaagaatt gcgcatggaa ccgaggacta
     5881 cagtccggga aatctgccac cggaggcgag gattgttatc acgccttacg acgatcggga
     5941 aaaagatgcg acggcgatga cgcgcgcgct cgctgcggaa gccattttaa aatctgctca
     6001 cgcaaacacc caaggctctt tactgaattg tgtccgcggc accatcaacg ttggccggct
     6061 ctcttattat atttacttgt tgtcctgcat aaccgccttg atcgccgctt caggcgcgct
     6121 cgatcagcca tcgggaaccg gcatatggcc gattaccaag gggttaatcc tcttcctggt
     6181 caatctagtg gcctcgccct ttgcgaccct ctatgcggtg ggacagaact tctcacgtga
     6241 cctcatccgg atcgcctgga tagcgagcgg atttctgctg gcttatctgc tgatgaagat
     6301 cacggataag cggatgagcg cagtattctc gaaattttgg ttcgaacaac agaaaaactt
     6361 acgggtcgcc ctaaagcagg cgcgtcagga agccaatgac atcatttccg ccggactttc
     6421 ttcgccgcag catcccacgg cgcatatcga caaagagccc agaatccggg tctcctgaca
     6481 atgctcccgt gcgcgctctg cttccgccga cgaaactcta gcctcgccgc ctgcttaatt
     6541 gccaagcatc tttttgacgc gatcgacgac ttctttgggc tgatccatga ccttattgat
     6601 tagttcttcc aactcttcgc cgggtgttgg atcgacatcc atcttggcct tttcggtttc
     6661 gaccagaagc tccggatcct ttagcgcctt gacataggct tcgcgtaatg tcttcacccg
     6721 ctccggcggt gtgcccggcg ttaccagcat cggccggccc agatcgccgc cggttaatat
     6781 cacctgagcg acgcgccggc tggcctcggg gctcttgtac tcgtcaaaca attcataaat
     6841 cgtcggcgta tccggcaggc gcgcatcgcg cttgcggccg gtctgaatga tgtgacgatc
     6901 gaagtctttt ttgtgccagc tgttgaaggg ctcgcggccg aaatgcgccg atatgttgtg
     6961 ggcgcggcag acaacttcat tcttttcaac cgcgagatct atctccgagc ctcccggata
     7021 accgataacg gtattgaatt tcgccccgat agagtcctcg agcaacctcg ccagcagata
     7081 gtcggtgctt acggttccgg acgagccgca tttcggcggc tccttggcct tgatgatgtc
     7141 ccggatcgac tgatatgggg catccttgct catgtagagc acaacactct cgcttgccgg
     7201 agaaccgatc cagccgaact tgcgaacgtc gaactgcact tc
//
LOCUS       DASUVS010000384         9025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1478670, whole genome shotgun sequence.
ACCESSION   DASUVS010000384 DASUVS010000000
VERSION     DASUVS010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9025
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478670"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1255
                     /locus_tag="VF182_20565"
     CDS             80..1255
                     /locus_tag="VF182_20565"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX6439521.1"
                     /translation="MKGCLDGIRVLELGNFISGPYGAMLLADMGAEVIKVENPKGGDP
                     FRGWDLGGDQPNFWAYNRGKKSVTLNLQTAEGKEIFYGLVKQADVVMDNYRPGVTKKL
                     GIDFESLQKVNEKIICCSITGTGPTGPYVKRPAYDTVGQGMGGMMSVLMDPQNPKPVG
                     PAYADCLSGMFSALGILGALTARARTGKGQQIDATMVGSIMAFLNAPATETLAHGKIP
                     GPYTRPRQSQTYAFACADGALLAIHLSSPQKFWEGLCKTVGHPELIEDPRFKTRPDRR
                     SNYDALSQTFAAFFKQQPRSAWVEALAVNDVPHTPVYNLKEVFEDPQVKHMGMEIAIE
                     RENKPTIRTVKFPLTHSETPSPHPAPPPELGEHNHEFLKSLGFDETKIAALKEKGVI"
     gene            1389..2564
                     /locus_tag="VF182_20570"
     CDS             1389..2564
                     /locus_tag="VF182_20570"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX6439522.1"
                     /translation="MNGCLEGIRVLELGNFISGPYGAMLLADLGAEVIKIENPQGGDP
                     FRGWDLGGDQPNFWAYNRGKKSVTLNLQTPEGKEIFLGLVKDADVIVENYRPGVTKRL
                     GIDYESLSKLNPQLIYCSITGTGPTGPYAKRPAYDTVGQGLGGMLSLLMEAKDPRPIG
                     PNYADSLSGVFATVGVLSALVARSKTGKGQQVDTTMAGSVLAFLNAPATDALATGKIP
                     GPYTRPMQSQTYAFSGSDGLPFAIHLSSPQKFWEGLCKSAGHAEMIDDPRFNTRPNRR
                     KNYDELNRVFTDFFKEKPRAYWLEQLEANDVPHTPVYNMLELFEDPQIKHMGLEVMIE
                     RKGKPNIRTVKFPNEYSATKSPHPGPPPELGEHNAEFLTALGYDGKKIAELKDQGVI"
     gene            2769..3947
                     /locus_tag="VF182_20575"
     CDS             2769..3947
                     /locus_tag="VF182_20575"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6439523.1"
                     /translation="MRRYDVVIIGGGAAGLMCAIEAGKRGRRVLVIERSSQIGKKILI
                     SGGGRCNFTNLHVTPENFLSANPHFCKSALARYTPSDFIAMVERHGIAYHEKERGQLF
                     CDLSAKPIVTMLADECRQADVRIEVNGVVTSVERAGKGFTIETGSGPIHGASLVIASG
                     GLSIPKMGATNFGYELAKRFGHSVVPTRPALVPLTFDADDLARYQDLSGIALAVDASC
                     NGQSFSAPMLFTHRGISGPAILQISSYWNRDAELRLNLLPGMEAAEWLLARQQSRPAA
                     ELKSVLSEKFPKRLAERLAEIGFDSRPLRQYRAAELRQIAGQLQNWVFHPIGTEGYRT
                     AEVTAGGVNTDELSSTTMESKKTPGLYLIGEVVDVTGHLGGFNFQWAWASGHAAGQAV
                     "
     gene            4079..5641
                     /locus_tag="VF182_20580"
     CDS             4079..5641
                     /locus_tag="VF182_20580"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF039846.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX6439524.1"
                     /translation="MKISLGPILSFRGVKTVGWSLSALIVTDILSTPPELIFQSGNKK
                     GIKRAAKLLKAFPDSNPTFQAWRFDFAVKQTARAQPVSYTIGGVENIFVVPALDATPN
                     LVYASCNGFSSMRLLNDTQDANRLWRQVAEKHEARPYHLLMMGGDQLYCDSMWDQLVP
                     LHEWARLPLDQRIKQPFTDEMRSVVDQFYATVYVERWSQAEPARVFAQVPNIMMWDDH
                     DIFDGWGSYEAELQQCDVYRGIFAIAREYFALYQLQLAREETHPMSIEAQQAFSLGGE
                     IGGGVTLLALDMRSERTKSQVISPESWKAIYDWLDATSPRKKSRDASARHLLLMSSIP
                     VVHPEFALLEKLLGIFPGRQQLEDDLRDQWLSVPHRQERLRLVHRLLAFADAKKCRVT
                     VLSGDVHVGALGIIESSRSTADDPSTQVINQLTSSGIVHPPPAGVVLFFLETVTGKKM
                     ELDRDLYSEMVEFPGTHHHFIGARNWLSLEPDEAGRIWANWYVETEPYPYTKVIHPID
                     FKMPAPSVPSES"
     gene            5744..6220
                     /locus_tag="VF182_20585"
     CDS             5744..6220
                     /locus_tag="VF182_20585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6439525.1"
                     /translation="MDFENKNWREHGVKIIPSAQLDLNTAQTPGMTRAAAITHARAGA
                     NKLWAGTVTIHPDAKTGPHHHGELESVIYVVSGKARMRWGESLEYIAEAGPGDFVFVP
                     PFVPHQEINASTVEPLFCVVVRSDQEPIVINLDIAASEKPESVEWIDPNHPPAPVK"
     gene            6181..6693
                     /locus_tag="VF182_20590"
     CDS             6181..6693
                     /locus_tag="VF182_20590"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6439526.1"
                     /translation="MDRSESSAGAGQIIMRQATENDLNFLAECYVKIACHMKAGEQDF
                     YIARLPDAPDETVRNHVARYVGREDALTLVEEIEETPIACLLASIGGASFSASRVGKS
                     GHIAVCWVEPVYRRAGLAGKLVSAAEDWFRQKEVAVVELSYMVKNELAAMSWQRLGYQ
                     PFRVFAYKQL"
     gene            complement(6787..7497)
                     /locus_tag="VF182_20595"
     CDS             complement(6787..7497)
                     /locus_tag="VF182_20595"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6439527.1"
                     /translation="MKILCRDLNPDKDGIPGYIAHPERKEPGPGILIVHHHYGVTGHL
                     KSVSCDFAKLGYTTVALGIYKLLGEADGVHEAQKKTTDGQFVEVIQQGWRYLTRRNDV
                     APKRCAVVGYCMGGRIGIHFVAATPDVRSFVGYYPSVRDEGPSQLRPRHPNEAVKDFK
                     CPSLIFFGGNDHVASVAVQDRLKASLHANGQPLDWHFFQHAGHGFALGDGDCYDPRLA
                     GLAWTITSEFLERELGSE"
     gene            complement(7605..8453)
                     /locus_tag="VF182_20600"
     CDS             complement(7605..8453)
                     /locus_tag="VF182_20600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX6439528.1"
                     /translation="MKRTGIKPAIGSLVLVIASVILPRPSAAAQSKIGAKAAVLMNAQ
                     TGKILWEQNQDLPLPPASTVKILTALVVLDHSRTSDLVTIPAEAVRVSGANTKLRAGE
                     KITAGDLLYAMLLGSGNDAAMALALHTAPTLDHFVERMNQKGHTLGAVRSRFVNPTGM
                     PHPKQLATAADLALITQAALENSEFRKIVSSRTYPWTSRAWQGTVKNSNKLLSSYDGA
                     IGVKTGSTREAGYCLVAAAQRDGQTYIAVVLKSQEKTVWNDAAQLLDYAFKHRSLEAG
                     RASSIR"
     gene            complement(8494..>9025)
                     /locus_tag="VF182_20605"
     CDS             complement(8494..>9025)
                     /locus_tag="VF182_20605"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX6439529.1"
                     /translation="TIVYVTHDQAEALTLGEKIIVLHQGIIQQIGSPSDIYQRPANTQ
                     VASFVGNPQMNLLHGTVDENGSVFQCGSLRLNLAAIFGRSLPQLAGNSVAIGVRPEDL
                     QLVDAAGQSWTGGDVELIEDLGSDRFLHVRCASVELIARAGRESTIKQGDPVGLSVAP
                     EQLHIFKDDRRCMPEP"
BASE COUNT         2019 a         2428 c         2580 g         1998 t
ORIGIN      
        1 caatatcgaa atcgcccacg actgtctatc ggacgtcgct gccggcgaca gggtgagtaa
       61 aaattaaaga ggtaaacgca tgaaaggttg tttggacggc atcagggttt tggagcttgg
      121 caattttatt tcgggccctt atggggcgat gttgcttgcg gatatgggcg ccgaagtcat
      181 caaagttgag aatcccaagg gcggcgatcc ttttcgcggt tgggatctgg gcggcgatca
      241 acccaatttc tgggcttaca accgcggcaa gaaaagcgtg accttgaatc tgcaaaccgc
      301 cgaaggcaag gaaatttttt atggcctcgt gaagcaggcc gacgtagtca tggacaatta
      361 ccgtccaggc gtaactaaga agctgggcat cgatttcgaa tcgctgcaga aggtcaatga
      421 aaaaattata tgctgctcga tcacaggtac cggaccgacc gggccgtacg tcaaacggcc
      481 ggcctatgac acggtaggtc aaggcatggg cggcatgatg agtgttctca tggacccaca
      541 gaatcctaag cccgtcggcc cggcctacgc ggactgtttg agcggcatgt tctcggcgct
      601 gggcattttg ggagcgctga cggcgcgcgc gcggacagga aagggacaac agatcgatgc
      661 cactatggtc ggttcgatta tggcattttt aaacgcgccg gcgaccgaaa ctctcgcgca
      721 cgggaaaata ccgggaccgt acacccggcc gcgccaatca cagacctatg cctttgcctg
      781 cgccgacggg gcgctgctgg cgattcactt atcgtcgccg caaaaattct gggaaggttt
      841 atgcaaaacg gtcggtcatc cggaattgat cgaagatccc cgatttaaaa cacggccgga
      901 ccggcgttcc aactatgacg cgctcagcca gactttcgcc gcgtttttca aacaacagcc
      961 tcggtcggcg tgggtcgagg cgctcgcggt caatgacgtg ccgcatacgc ccgtgtataa
     1021 cttgaaggag gtgttcgaag atccgcaggt taaacacatg ggcatggaaa tcgcgattga
     1081 gcgcgaaaat aagccaacga ttcgaaccgt gaagttccct ttgactcaca gcgaaactcc
     1141 atcgcctcat cccgccccgc cgccggagct tggcgaacat aaccatgaat tcttgaagtc
     1201 cttgggcttt gacgaaacaa aaatcgccgc gctcaaagag aagggcgtga tctaagaacg
     1261 atattcaccg ctgagacaca gagttcgccg agagttttct gatcatttct ctgcggcctt
     1321 tgcgcctctg cgggtggatc atctccaatc atcaaatgtt gcatcaaaat tgcgagtagg
     1381 agagcacgat gaatggttgt ttagaaggca tcagggtttt ggagctgggt aattttattt
     1441 ccggtcccta tggcgcgatg ttgctcgccg atttgggagc ggaagtcatc aaaattgaaa
     1501 atcctcaagg tggcgatccc tttcgcggtt gggatctggg cggcgatcaa cccaatttct
     1561 gggcttacaa ccgcggcaag aaaagcgtga cgttgaattt gcaaaccccg gaagggaagg
     1621 agatctttct tggcttggtc aaagatgccg atgtgattgt ggagaactac cggccgggag
     1681 tgaccaagcg attgggcatt gattacgaga gcctgagcaa gctcaacccg cagcttattt
     1741 actgttcgat caccggcacc ggcccgaccg gtccgtatgc caagcggccg gcctacgaca
     1801 cggtgggcca gggacttggc ggcatgttga gtttgctgat ggaggcgaaa gatccccgcc
     1861 cgatcggtcc caactacgcc gatagcttga gcggcgtgtt cgccactgtc ggtgttctaa
     1921 gcgcgttggt ggcacgatcg aaaaccggca agggccagca ggtcgatacc acgatggcgg
     1981 gttcggttct ggcttttttg aatgcgccgg ccaccgatgc gcttgccact ggaaaaatcc
     2041 ccggaccata cacgcggccg atgcagtcgc agacttacgc cttcagcggc tctgatggct
     2101 tgccgtttgc gattcaccta tcgtcgccgc agaaattttg ggaaggtttg tgcaaatccg
     2161 ccggccacgc cgaaatgatc gatgatccac gcttcaacac ccgaccgaat cgtcggaaga
     2221 attatgatga gttgaacaga gtcttcaccg atttcttcaa agagaaaccg cgcgcctact
     2281 ggctcgaaca gctcgaagct aacgatgtgc cgcacacgcc ggtttacaac atgctcgagc
     2341 tgttcgaaga cccgcagata aaacacatgg gtctggaggt aatgatcgag cgcaaaggca
     2401 agccgaacat caggacggtt aaattcccca acgagtacag cgcgaccaaa tcccctcatc
     2461 ccgggccgcc gcccgagctc ggcgagcaca atgcggagtt tttgaccgcg ctgggatatg
     2521 acggcaagaa gatcgcggag ctcaaagacc agggcgtgat ctaaagacga tattcaccgc
     2581 ggagaaacag agttcgcaga gagtttttat gagcgtttct ctgtgtcctc ggcgtctcta
     2641 tggtgagtcg ggccgcaatc ggcaatctcc ctcgccaaag tcgcacaata atccaatcta
     2701 ttgcccgagc cgtatctctg gtactcaaag aatggtagcc cagctcaaga gctttctcgt
     2761 ttcattcaat gcgccgctat gatgtagtga ttatcggcgg cggcgccgct ggtttgatgt
     2821 gcgcgatcga ggcgggcaaa cgcgggcggc gcgttctggt tatcgagcgc agcagccaga
     2881 tcggcaaaaa gatccttatc tccggcggcg gccgctgtaa cttcaccaac ctgcatgtca
     2941 ctccggaaaa tttcttgtcg gccaatccgc atttctgcaa atcagcgctg gctcgctata
     3001 caccttcaga cttcattgcc atggtcgagc gccatggcat cgcctaccac gaaaaagagc
     3061 gcggccagtt gttttgcgat ctctcggcca agccgatcgt gacgatgctg gcagacgaat
     3121 gccggcaggc cgacgtgcgc atcgaggtaa acggcgtggt gacgtccgta gagcgagcgg
     3181 gcaagggttt tacaatcgag accggcagcg gtccgattca cggcgcgtcg ttagtcatcg
     3241 cttcaggcgg tctgtcgatc ccgaagatgg gcgccaccaa tttcggctat gagctcgcca
     3301 aacgatttgg acatagcgtc gtgcctaccc gcccggcgct ggtgccgctc acattcgatg
     3361 ccgatgatct ggcgcgctat caagatttaa gcggcatcgc tttagccgtt gacgcaagtt
     3421 gcaatggcca aagttttagc gcgccgatgc tgtttaccca ccgcggtatt agcgggccgg
     3481 cgattttgca gatatcttct tactggaacc gggatgcaga gctgcggctc aatcttttgc
     3541 cgggcatgga ggcggcggag tggttgctgg cgcggcagca gagccggccg gcggcggagc
     3601 tcaaaagcgt gctgagcgaa aaatttccca agcggctggc cgagagattg gctgaaatcg
     3661 gattcgacag ccggccgctg cgacagtatc gcgccgccga attgcggcaa attgccggcc
     3721 aactacagaa ctgggtattt cacccgatcg gcaccgaagg gtaccgcacc gccgaggtca
     3781 ccgcgggagg cgtcaacacc gatgagcttt cctcgacaac catggaatcc aagaaaacac
     3841 ccggccttta tttaataggc gaagtggtgg atgtgaccgg ccatctgggc ggattcaatt
     3901 ttcaatgggc ctgggcatcc gggcatgccg cggggcaggc agtctagaac tgttcgacgg
     3961 ttcaacggtt cgaacgtgca acagcagagt tcagattagg tgaacaccgt ttgtgcaaag
     4021 attatccagc gccgattaaa gaacgaagtc gtggattgtt taggataggg gagcgacgat
     4081 gaaaatttct cttgggccga ttcttagttt tcggggggtg aaaacggttg ggtggagttt
     4141 aagcgcgctg atcgtgaccg acatcttaag caccccgcct gaattgatat ttcagagtgg
     4201 caacaagaag ggaattaagc gggcggccaa acttctaaaa gcgtttcccg actccaatcc
     4261 gactttccag gcctggcgct tcgatttcgc ggtcaaacag actgcccgcg cacagccggt
     4321 cagttacacc atcggcggcg tagaaaatat ctttgtagtt ccggcgttgg atgccacccc
     4381 caatctggtt tatgcatcct gcaatgggtt ctccagtatg agattgctaa acgacaccca
     4441 agacgccaat cgattatggc gccaggttgc ggaaaagcat gaggccagac cgtaccatct
     4501 tttgatgatg ggcggagatc agctctattg tgattcgatg tgggaccagc ttgttccgct
     4561 tcacgaatgg gcgcgtttac cgcttgacca acggattaaa cagccgttta ccgatgaaat
     4621 gcgctccgtg gtggatcagt tctatgcgac ggtctatgta gagagatggt cccaagccga
     4681 gcccgctcgc gtgtttgctc aagtgccgaa catcatgatg tgggacgatc acgatatctt
     4741 tgatggttgg ggatcgtacg aagcggagct tcagcaatgt gatgtttata ggggaatttt
     4801 tgctatcgcg agagaatact ttgcgcttta ccaattgcag ctcgctcgcg aagagaccca
     4861 cccgatgtcg attgaagcgc agcaggcttt tagccttggc ggtgaaatag gcggcggcgt
     4921 gacactgctg gcgttggaca tgcgaagtga aagaaccaag agccaggtca tcagtcccga
     4981 atcctggaag gctatctatg attggctgga tgcaacgtct ccgcgcaaaa agagtcggga
     5041 tgcatcggct cgacaccttt tactgatgtc cagtattcca gtagttcatc ctgaattcgc
     5101 gctgctcgag aaactcctgg gaattttccc tggccggcaa cagctggagg acgatttgag
     5161 agatcaatgg ctgagcgtgc ctcatcgcca ggaacggttg cgtctggttc atcggttgct
     5221 cgccttcgcc gatgccaaga aatgccgggt caccgtgctc tccggcgatg ttcacgtcgg
     5281 cgctcttggt atcatcgagt cgagccgctc gaccgcagat gatccgagca cccaagttat
     5341 caatcaactg acctcatccg gcatcgtgca tccacctccg gccggcgtag ttcttttttt
     5401 cctggaaact gtaaccggca agaaaatgga actcgatcgc gatctttaca gtgagatggt
     5461 ggaatttccc ggaacccatc atcattttat cggcgccaga aattggctca gtctggaacc
     5521 cgatgaggcg gggagaatct gggccaactg gtatgtcgaa actgagccgt acccttatac
     5581 caaagtcatt catccgatcg atttcaaaat gccggcgcca agtgtcccaa gcgaatctta
     5641 ggtggtgagg ctgttgtcat catggaatgc taatcatcaa aactatcctg tgctgcgtgc
     5701 tcgatcatgc cgatcgccgc agtgaaggcc tcaggaggga cgaatggatt ttgaaaataa
     5761 gaattggcgc gagcatggag tcaaaatcat tccgagcgcg cagctcgatc tcaataccgc
     5821 gcaaactccc gggatgacgc gcgccgccgc gatcacccac gcgcgcgccg gcgcaaacaa
     5881 gctctgggcc ggcaccgtga cgatccatcc ggatgccaaa accggcccgc accatcacgg
     5941 cgaattggaa agcgtcatct atgtggtcag cggcaaggcc cgaatgcgct ggggcgaatc
     6001 attggagtat atcgccgagg ccgggcccgg tgattttgtc tttgtgccgc ccttcgtacc
     6061 gcatcaggag atcaatgcca gcacggttga gcctttgttt tgcgtcgtgg ttcgaagcga
     6121 tcaggaaccc atcgtcatca atctggatat cgctgcctcc gagaagccgg aatccgtcga
     6181 atggatcgat ccgaatcatc cgccggcgcc ggtcaaataa tcatgcgcca agcaactgaa
     6241 aatgatttga actttctcgc cgagtgctac gtgaagattg cctgccatat gaaagccggc
     6301 gagcaggatt tctatattgc gcggctgccg gacgcacccg acgagaccgt ccgcaatcac
     6361 gtggcgcgtt atgtcggccg cgaagacgcg ctcaccttgg tagaggaaat cgaagagacg
     6421 cccatcgctt gcctattggc cagcataggc ggcgcatcgt tctccgcgtc gcgtgtcgga
     6481 aagtcgggtc acattgcagt gtgctgggtc gaaccggtat accggcgcgc cggcctggcg
     6541 ggcaagctgg tcagcgccgc cgaagattgg tttcggcaaa aggaagtcgc tgtcgtcgag
     6601 ctttcatata tggtcaagaa cgaactcgcc gccatgtcat ggcagcgact cggctatcaa
     6661 ccgtttaggg tttttgccta caaacagttg tgagtacaag gcggcaggat gaaagcggaa
     6721 agatgaaacg aggattccaa gcggcttttt cagatttcat ctttcataat tcatctttga
     6781 tctcgcctac tcgctgccca actctcgttc caaaaactcc gacgtaatcg tccaggcgag
     6841 cccggccagc cgcggatcat agcagtcgcc atcgcccaga gcgaagccgt gacccgcgtg
     6901 ctggaagaaa tgccaatcca gtggttggcc attggcatga aggctggctt tcagacgatc
     6961 ctgcacggcg acgctggcga catgatcgtt gccgccaaag aagatgagcg acgggcattt
     7021 aaaatctttc acggcctcgt tgggatgacg cgggcgtaat tggctgggcc cttcatcgcg
     7081 cacggaagga taataaccta cgaagctgcg cacgtccgga gttgccgcga cgaaatggat
     7141 gccgatgcgc ccgcccatgc agtagccgac tacggcgcag cgtttggggg cgacatcgtt
     7201 tctccgggtc aggtagcgcc agccttgctg gatcacctcg acgaattgtc cgtcagtggt
     7261 ttttttctgc gcctcgtgaa ccccgtctgc ttcgccgaga agcttataaa tgccgagagc
     7321 gactgtggta tagcccagct tggcgaaatc gcagctcact gatttgagat ggccggtaac
     7381 gccgtagtga tgatgcacga ttaatattcc cgggccgggc tctttgcgtt cagggtgggc
     7441 aatgtatccg gggattccgt ctttatcggg gtttaaatca cggcaaagga tcttcatcgg
     7501 cgcgcttctc cttgggcagt ttcaatctct ttctggtatc acgactggcc cgcgcttgtc
     7561 attcactttg ggctatgatc ttggggttca attcaaaacc tgcgtcaacg aatcgatgag
     7621 gcgcggcctg cttccagcga gcgatgctta aacgcataat cgaggagctg cgcggcatcg
     7681 ttccaaaccg ttttttcctg gctcttcaaa acgacagcga tgtaagtttg accgtcccgt
     7741 tgggccgctg ccaccagaca gtagccggct tctcgggtgc tgccggtctt gacgccgatg
     7801 gcaccatcgt agctggatag caacttattg gaatttttaa ctgtgccctg ccatgctctg
     7861 ctcgtccacg gataggttct gctgctgacg atcttacgaa actccgaatt ctccagagca
     7921 gcttgagtga tcaacgcgag atccgctgcg gtagctagct gtttggggtg aggcatgccg
     7981 gtcggattga caaagcggga acgaaccgcg cccagcgtat gccccttttg attcattcgt
     8041 tcgacgaaat gatcgagggt tggcgcagta tgaagggcta acgccatcgc ggcatcgttg
     8101 ccggaaccca acagcattgc atacagcagg tccccggccg taattttttc tcccgctcta
     8161 agcttcgtgt tggccccgga aacgcgcaca gcctcggccg gaatggtgac aaggtcgctg
     8221 gtccggctat gatccagcac taccaatgcc gtgaggattt tgactgtgct tgccggcggc
     8281 agcggcagat cttgattctg ttcccagaga atctttcccg tttgcgcgtt catcagcacc
     8341 gccgctttgg ctccgatctt cgattgggca gcggcgctgg gtcgtggcaa aatgaccgag
     8401 gctatgacca acacaagcga gccgatcgct ggtttgatgc cggtgcgctt catcgatgaa
     8461 agctctttta gctcaggagc gcgtgcatga agctcaaggt tcaggcatgc accgccgatc
     8521 atccttgaag atatggagct gctccggcgc gacgctcaag ccgacgggat ctccttgttt
     8581 aatagtcgac tctctgccgg ctcgagcgat cagttcgaca cttgcgcatc tcacgtgaag
     8641 gaagcggtcc gagcccagat cttcgattaa ctcgacatcg cccccggtcc agctttgtcc
     8701 agccgcgtcc accagttgca aatcctccgg gcgaacaccg atagcaacag agttgccggc
     8761 tagttgcggc agggatctgc caaaaattgc tgcgaggttc aggcgcaggc tgccgcactg
     8821 aaaaaccgat ccattttcat ccaccgttcc gtgtaacaga ttcatctgcg ggttaccgac
     8881 aaatgatgcg acctgcgtat tagccggtcg ttgataaatg tcagagggcg agccgatctg
     8941 ctggatgatc ccttggtgca ggacgataat cttttcaccc agggttagcg cctcggcctg
     9001 gtcatgggtg acgtagacaa tcgtg
//
LOCUS       DASUVS010000385        10098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_386493, whole genome shotgun sequence.
ACCESSION   DASUVS010000385 DASUVS010000000
VERSION     DASUVS010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10098
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386493"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..622
                     /gene="nuoE"
                     /locus_tag="VF182_20610"
     CDS             149..622
                     /gene="nuoE"
                     /locus_tag="VF182_20610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011715170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX6439530.1"
                     /translation="MAVEFSPETLKKFEETVRRYPKKEAAMLPVLYLAQQEFGYLGPE
                     AIDYVAKLMDQSPARVHGVVSFYTMLNMKPIGRHHIQVCRTLSCALGGAEKITDFIRK
                     KLDIDLGQTTADGRFTLSEVECLASCGTAPMMQINDDYYENLNEEKVTEILDSLK"
     gene            639..1940
                     /gene="nuoF"
                     /locus_tag="VF182_20615"
     CDS             639..1940
                     /gene="nuoF"
                     /locus_tag="VF182_20615"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX6439531.1"
                     /translation="MEKILLRNIDVADSHTLTTYQARGGYRAWEKVVRSMKPEQLIDE
                     VKASGLRGRGGAGFPTGMKWSFVPKDSPKPKYMICNADESEPGTFKDRLLIEKDPHAL
                     IEGTLIAAFGIQSHTAFIYIRGEMAFGTQVLERAAEEVKRAGLTGKNIFGSGYDLDLI
                     VHRGAGAYICGEETALLSSLEGGRGWPKVKPPFPATHGLFGCPTVVNNVETLAALPWI
                     IDHGAAAYAAMGTEKSKGTKLFSVSGHISKPGVYEVEMGYPFKKFLEEDCGGVPNGRK
                     LKGVIPGGASMPVFRPEEIENVNLDYESVQAAGSLLGSGGVIVMDDSTCMVRAAWNVS
                     RFYAHESCGQCSPCREGCHWMEKIFHRIEHGQGDKGDMDLILNVSGNIMGNTICPFGD
                     AAAMPAAAFIRKYRDEFEQHIVERGCTVRNGQPSFERSRVA"
     gene            1967..3556
                     /locus_tag="VF182_20620"
     CDS             1967..3556
                     /locus_tag="VF182_20620"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6439532.1"
                     /translation="MDPVKLIIDGQEISTVKGKTVIQAAAEAGITIPHYCYHAKLSIA
                     GNCRMCLIEIEKMPKLQIACNTQVAEGMTVLTQSPKVLSVRKAVMEFLLINHPIDCPI
                     CDQAGECWLQDYYMTHDLQDSRFKERKERDRKREIFGPNIVFDGERCIKCTRCVRFLD
                     EITHTGELTVDNRSDDSTIALFPGTVLDNPLSANVVDICPVGALTDRDFRFKVRVWYL
                     QKTPSICPGCSTGCNISAEHYQNRIARFKPRVNEAVNSHWLCDEGRYCFHDLTGGERL
                     TTPMIRQEGGLVPTTWEVALRAVISGLRSAGSLAGILSGRNTNEEAFLFAKLMKKLSP
                     AAALEVFYQERELTEVEKILVSPDRSPNFRGGAEMGVASDGGFEPLMQKLVAGNFGGA
                     YVVGEDLTPANVNGAELRSALGKLSFLVVQDIRLTETAKAAHVVLPSTHFGEKEGTYT
                     NRKGRVQRLNAALVAPEGALQDCEIFIRLLEAAGERASYSSAEEIFQALSQEIPSYRG
                     LNYDVIGDQGIQLGSGGADPS"
     gene            3553..4692
                     /locus_tag="VF182_20625"
     CDS             3553..4692
                     /locus_tag="VF182_20625"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX6439533.1"
                     /translation="MIVDIGIVAIKAFLVLFMVLNLAGVLGWVERKGSALIQDRIGAN
                     RASIFGFAGMGLINTLVADPLKFLTKEDFIPPAGDRFLHTLAPCLALFPALITFAVIP
                     FGDVLQIGERVINLQVANLNIGILYIFAMTSLGVYGLVIGAWASNNKFSLLGGVRGSA
                     QMISYEVALGLSVIGILMVYGSLDLQEIARSQGALIRDLLPDSWPFLKTLVGWLPAWG
                     IFLQPLAFLLFFTAAIAETRRLPFDLPEAESELVAGSHVEYSGGKFLMFFAAEFAEIV
                     TVSGLVTTLFFGGWQVPYLLRDGFHFPWGGTLLLPHLSIVLLQVGAFTFKVIFLCWVQ
                     ILLRWSVPRFRYDQVMRLGWKMLLPAALVNVMITAFVILYIDSIP"
     gene            4697..5191
                     /locus_tag="VF182_20630"
     CDS             4697..5191
                     /locus_tag="VF182_20630"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX6439534.1"
                     /translation="MSGPEIIFFIVAGLLIVSSILVVFQRNVVHSAVALVAALFLIAV
                     LFLSLHAPMVGVLQILVYAGAIMVLFLFVIMLLNPVGLEQRRNLWWVAGSVSGLLLIA
                     MLAPVLFNLGSAQDPMVATELFGSPEELARSLFTDFVLPFEIASVLLLVAIIGAVVLA
                     NKEP"
     gene            5199..5501
                     /gene="nuoK"
                     /locus_tag="VF182_20635"
     CDS             5199..5501
                     /gene="nuoK"
                     /locus_tag="VF182_20635"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX6439535.1"
                     /translation="MVPLEYYLVLSAIIFAIGVIGVLIRRNLIVVLMSIELMLNAVNL
                     TFVAFSNSLGSMDGQVIVFFVMAVAAAEAVVGLAIIISVFRHRQSLDPQEMQLLKW"
     gene            5495..7423
                     /gene="nuoL"
                     /locus_tag="VF182_20640"
     CDS             5495..7423
                     /gene="nuoL"
                     /locus_tag="VF182_20640"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX6439536.1"
                     /translation="MVASTATVAALDLLRWIPLIPLGAGLFNVFFGRKLGGKTAGGIA
                     SAAVGVSFGLALYVFWRLPATGVVRDLWYTWIQSGSFQVNIAFQVDALSAVMLLIVTG
                     IGFLIHVYSLAYMAHDESMARFFIYLNLFIFFMLLLVMADNLLLLFVGWEGVGLCSYL
                     LIGFWYQDRQNVIAGNKAFIVNRIGDFGFIIGIFLLVTELGRQGIWTLDFTELQKNVQ
                     IIGPAKITLITLLLSVGAAGKSAQIPLFVWLPDAMLGPTPVSALIHAATMVTAGVYMI
                     ARLHFLFILAPYTLPIIAVVGAATAFFAATVALAQNDIKKVLAYSTVSQLGYMFLAVG
                     IGAFSAAIFHVFTHAFFKACLFLGSGSVIHALDGEQDMRKMGGLRNALPQTYWTYLIA
                     TLAIAGIPFTAGFFSKDLILWKAFEDGSYGLWSIGIITAGLTAFYMFRQLFMVFHGNF
                     RGDDHTKAHIHESPPLMTVPLVILAAGSIFSGWLGAPEYLWGSVWDDWLQPVFGAGAG
                     HSVEHAPVSTELLVTAITLAVVALGIYLAYARYGRARATVPAAADTGLYRLLLDSYGI
                     DEIYDRLVVRPYTAASDWLARVFDPVVIDGVINGVARVTRGFSLIWREVQTGNIQHYL
                     IGFLAGTLALLYYYLRQQ"
     gene            7426..8973
                     /locus_tag="VF182_20645"
     CDS             7426..8973
                     /locus_tag="VF182_20645"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX6439537.1"
                     /translation="MSRLPGDISLLLLLPLIGALVLLLIPRQQRQTLFTLALIASIAA
                     FVWSLRLFQAFDAGPGQMQFVEKIPWMPAFGIGYIVGIDGISLFLVLLTTFLMPLAIA
                     ASWTVQDRVKEYLFFMLVLETGMLGAFVALDLFLFYVFWEVMLVPMYFLIGVWGGTRR
                     IYAALKFVVYTMAGSLPMLVAIIYLASRYAHVNQAMTFDVMQLYSLRLPVNEQIWLFL
                     AFGLSFAIKVPLFPFHTWLPDAHVEAPTAGSVILAGVLLKMGTYGFLRFAIPLFPDAA
                     AVFTPLIIALAVIGIIYGACVAMMQPDLKKLVAYSSVSHLGFVMLGLFALNIQGIQGG
                     IYQMVSHGLSTGALFLLVGMLYDRRHTRMISDFGGLWHQLPLLSVAFLIVTFASIGLP
                     GLNGFIGEFLILLGAFSLTPRWAAAATTGVILGAIYMLWMFRRVFFGPVTHPENQKLV
                     DLNSRELLILAPVIALIIIMGVYPQPFLSRMKTSVDVSLQRIHNSKAAPVATNHAADR
                     EINTNGR"
     gene            8963..>10098
                     /locus_tag="VF182_20650"
     CDS             8963..>10098
                     /locus_tag="VF182_20650"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX6439538.1"
                     /translation="MDVNLVPLLPAAQVLLAALVVMLLDLFFADHEKPLLAGITVFGL
                     ALCAVEAVLLWGSREGAFGETFLLDNFSLFFTQLFVGAAILTVLSSVRYVREAGIHQG
                     EFYSLILFATAGMIVMAAANDLIVFFLGLETMSVAVYVLTGIWRSSSRSAEAAMKYFL
                     MGSFATGFLLYGIALIYGATGTTNLTQVSGYLLEPQEQWPRYLSVGGLLLLVGFAFKV
                     GAVPFHFWIPDVYEGAPTPVTGFMSVAVKAAAFAAWARILMHQLSPLDSDWAYAVRVL
                     AIATMTFGNLLAISQSSVKRMLAYSSIAHAGYILIGIVAGEEWGSTSLLFYLATYVPV
                     TFGAFAVITALTEKDRPKEEYGHFAGLGFKRPFLAMAMTLFMLSL"
BASE COUNT         1935 a         2688 c         2867 g         2608 t
ORIGIN      
        1 cggtagcgtc aaagtagaat cgtgtcatcg cgatctttgg ccgaaatgat caatatgatc
       61 gggggggaat gcgaccggtc gcagcctgca cagagttttc cagcacagcg ggttagataa
      121 gagttgtttt aaccgagaag aataacgcat ggcagtagaa ttttcaccgg aaacgctcaa
      181 gaaattcgaa gagacggtgc ggcggtatcc taagaaagag gcggcgatgc ttccggtgct
      241 gtaccttgcc cagcaggaat tcggctatct gggtccggag gcgatcgact atgttgctaa
      301 gctgatggat caatcgccag cgcgggtgca cggggtggtg agcttctata ccatgttaaa
      361 catgaaaccc atcggccggc accatattca agtatgccgc actctgtcat gcgcgctggg
      421 tggggcggag aaaattaccg atttcatcag gaaaaagctc gacattgatc tcggacagac
      481 cacggctgac gggcgattca cgttatcgga agtcgaatgc ctggcatcgt gcgggacggc
      541 gccgatgatg caaatcaacg atgactatta cgaaaacttg aacgaagaaa aggtaacgga
      601 aattctcgat tccttgaaat aactttactt tcagaccgat ggaaaaaatt ctgcttcgta
      661 acatcgatgt cgccgattcg cacaccctga ccacatatca ggcccggggc ggctatcgtg
      721 cttgggaaaa ggtcgtccgg agcatgaagc cggaacagtt gatcgacgaa gtgaaggctt
      781 cagggctcag gggcaggggg ggcgccggat ttcccaccgg gatgaagtgg agcttcgtgc
      841 ccaaagacag tcccaagccc aagtatatga tttgcaacgc cgacgaaagc gaacccggca
      901 cattcaagga tcgcttgctg atcgagaaag atccacacgc tctcatcgaa ggaacgctga
      961 tcgcagcgtt cgggattcaa tcgcataccg cgttcattta tatccggggc gagatggcat
     1021 ttggcaccca agtgctcgaa cgggccgctg aagaggtcaa gcgcgccggt ttgaccggca
     1081 aaaatatctt cggctcgggc tacgatttgg atctgatcgt tcatcgcggc gccggcgctt
     1141 atatctgcgg cgaagaaacc gctttgcttt cttcgctcga aggcggccgc ggctggccca
     1201 aggtcaaacc gcctttcccg gccacgcatg gactgttcgg atgtccgaca gtggtaaaca
     1261 atgtcgagac attagccgcg ctgccgtgga ttatcgatca tggcgccgca gcttatgccg
     1321 ccatgggaac cgagaagagc aaaggcacga aactctttag cgtcagcggg cacatcagca
     1381 agcccggtgt ttacgaagtc gagatgggat atccgttcaa gaagtttctt gaagaggatt
     1441 gcggtggggt tcccaacggg agaaaactca agggggtgat tcccggcggc gcatcgatgc
     1501 cggtttttcg gccggaagag atcgaaaacg tcaatctcga ttatgagtcg gtccaggcgg
     1561 caggcagctt gctcggctcc ggcggcgtca tcgttatgga cgattccact tgcatggtcc
     1621 gggcagcgtg gaacgtttca cgtttctacg ctcatgaatc gtgcggccaa tgcagccctt
     1681 gccgggaggg ttgtcactgg atggaaaaaa tttttcaccg catcgagcat ggccagggcg
     1741 ataaaggcga catggatctg attctcaacg tcagcggcaa cattatggga aatacgatct
     1801 gtccgtttgg cgatgccgcc gccatgccgg ccgcagcctt tataagaaaa taccgggacg
     1861 aattcgagca gcatattgtc gagagaggct gcacggtaag aaacggacag ccaagtttcg
     1921 aacgcagccg agtcgcttaa agaaggtcta gacgatttct tattccatgg atccagtaaa
     1981 acttattatc gacggtcaag agatcagcac cgtcaaaggc aaaaccgtta ttcaggcggc
     2041 cgcggaggcg gggattacga tcccgcatta ctgctatcat gcgaagcttt cgatcgccgg
     2101 caattgccgc atgtgtctga ttgaaatcga gaagatgccc aagctgcaga tcgcctgcaa
     2161 cacgcaggtc gccgagggga tgaccgtgct gacgcagagc cccaaggtac tatcggtgcg
     2221 caaggcggtg atggaatttc ttctgatcaa tcatccgatc gattgcccga tatgcgatca
     2281 ggcgggtgag tgctggctgc aggactatta catgacgcat gacctgcagg acagccggtt
     2341 caaggagcgc aaagagcgtg accgcaaacg cgagattttc ggccccaaca tcgtcttcga
     2401 tggtgagcga tgcatcaaat gcacccgctg cgtgcgcttt cttgacgaaa tcacccatac
     2461 cggcgagctg accgtcgata accgcagcga tgattcgacg atcgcgcttt ttcccggcac
     2521 agttctcgac aatccgctgt cggccaatgt ggttgatatc tgtcccgtcg gcgcgcttac
     2581 cgatcgcgat tttcgtttca aggtcagggt ctggtatctg cagaaaaccc cttccatttg
     2641 ccccgggtgc agcaccggct gcaatatcag cgcggaacat tatcagaatc ggatcgctcg
     2701 ttttaagcca cgggtcaatg aagcagtcaa cagccattgg ctctgtgatg agggccgata
     2761 ctgtttccat gatttgaccg ggggtgagcg gttgacgaca ccgatgattc ggcaggaggg
     2821 cgggcttgtt ccgaccacct gggaggtagc cttgcgcgcc gtgatcagtg gactacgctc
     2881 ggcgggttcg ctggccggaa tactttccgg ccgaaacacc aacgaagaag cgttcttgtt
     2941 cgccaagctg atgaagaagc tttcgcccgc tgcggctctg gaagtgttct atcaggaacg
     3001 cgagctgacc gaagtcgaga agatccttgt gagtccggac cgttcgccta atttccgtgg
     3061 tggcgccgag atgggggtcg cctctgacgg cggctttgaa cctttgatgc agaagctggt
     3121 cgcaggaaac tttggcggcg cctatgtagt gggcgaagac ttaacgccgg caaatgtcaa
     3181 cggagcggag ttgcgctctg ccttgggcaa gctatctttc ctggtggtcc aggatatacg
     3241 cctgaccgaa accgccaaag ctgctcacgt ggttttgccg tcaactcatt tcggcgagaa
     3301 agagggcact tacaccaacc ggaaagggcg cgtgcaaagg ctcaacgccg cgctggtcgc
     3361 gccggaaggc gcgctgcaag attgtgaaat cttcatccgc ttgctggagg cggccggtga
     3421 gagagcatcc tactcgtcag cagaagaaat ctttcaggcc ttgtcacagg aaattccttc
     3481 ctatagagga ctcaattacg atgtcatcgg cgatcaagga attcagttgg ggagtggtgg
     3541 tgcggacccg tcatgatcgt tgatatcggc attgtcgcga tcaaagcctt cctggtgctt
     3601 tttatggtcc tcaacctggc cggagtgctc ggttgggtcg agcgcaaggg cagcgcgcta
     3661 attcaagatc gtatcggcgc caatcgggca tccatattcg gttttgccgg tatggggctt
     3721 atcaatacgc tggttgccga cccgctgaaa tttttgacca aagaggattt cataccgccg
     3781 gccggcgacc ggttcctgca tacgcttgcg ccttgcctgg ctctattccc ggctctaata
     3841 acctttgcgg tcattccctt cggcgacgtt cttcagattg gcgagcgggt gatcaatctt
     3901 caggttgcca atctcaacat cggcatcctt tacattttcg ccatgacctc tttgggcgtt
     3961 tacggtctcg tgatcggcgc ctgggcatcg aacaacaaat tttcgctctt gggcggcgtc
     4021 cgtggttcgg cgcaaatgat ttcctacgaa gtggcgctgg gtctttccgt gatcggtatt
     4081 cttatggttt acgggagtct agaccttcag gagatcgcgc gcagccaagg cgctctgatc
     4141 agagatttgc tcccggattc gtggccattt ctcaaaactc tggtgggttg gcttccggct
     4201 tgggggatct tcctgcagcc gctggcgttc ttactcttct ttaccgcggc cattgccgaa
     4261 accaggcggt taccgttcga tctaccggaa gcagaatcgg aattagtggc cggctctcac
     4321 gttgaatatt ccggtggcaa gttcctgatg ttctttgccg ctgagttcgc ggagatcgtc
     4381 actgtttccg gcttggtaac gacacttttc ttcggcggct ggcaggtgcc ttacctgttg
     4441 cgcgatggtt ttcatttccc ctggggcggg accctgctgt tgccccatct gtcgattgtg
     4501 ctcctgcagg tcggcgcgtt tacattcaaa gtgatctttt tatgctgggt gcagattctg
     4561 ctgcgctgga gcgttcctag atttcgttac gaccaggtga tgcgattagg ctggaagatg
     4621 ctcttgccgg cggcactggt caacgtaatg atcaccgcct ttgtgattct atatatcgac
     4681 tccatcccgt aaacctatga gtggtccaga gatcattttt tttatcgttg cggggttgct
     4741 gatcgtttcc tcgatactgg tggtgtttca acggaatgta gtacacagcg cggtggcgtt
     4801 agtagccgcg ttgtttctaa ttgctgttct ctttctgagc ctgcacgcgc ccatggtcgg
     4861 cgtgttgcag atcctggttt atgccggcgc catcatggtg ctctttctct tcgtcatcat
     4921 gctgttgaat cctgtagggc tggaacagcg ccgcaatctt tggtgggttg ccggctcggt
     4981 ttcaggcttg cttctgatcg cgatgttggc tccggttctg ttcaatctcg ggtctgccca
     5041 ggatccaatg gtcgccaccg agctttttgg cagcccggaa gagctggcca ggagcctttt
     5101 taccgacttt gttcttcctt ttgaaatcgc ctcagtcttg cttttggtgg caattattgg
     5161 cgccgtggtt ttggcgaaca aggagccgta agcgtcccat ggtgccgctg gaatattatc
     5221 tggttctgag cgcgatcata tttgccatcg gcgttatcgg cgttctcatc cgccgcaacc
     5281 tgattgttgt gttgatgtcc atcgagctta tgctcaacgc ggtgaacctg acctttgtcg
     5341 ctttttccaa ctcgctaggt tccatggacg ggcaggtgat tgtgttcttt gtcatggccg
     5401 tggccgctgc cgaagcagtg gtgggactcg ctatcattat ttccgtgttt cgccatcgcc
     5461 aatcgctcga tcctcaggag atgcagcttc ttaaatggta gcctccaccg ccaccgttgc
     5521 cgctttggat ttgctgcgct ggattccgtt gatccctctg ggcgccggac tgttcaatgt
     5581 cttttttggt cgaaagctgg gcggtaagac agccgggggc atcgcttccg ctgcggtcgg
     5641 cgtttctttc ggcttggcgc tttatgtctt ttggcggctc ccggccacag gtgtggtccg
     5701 cgatctttgg tatacctgga tccagtcagg ttccttccag gtcaatatcg catttcaggt
     5761 cgacgcctta tcggcagtca tgcttctaat cgtcaccggc attggtttcc ttatccatgt
     5821 ttattcgttg gcgtacatgg cccatgacga gagcatggcg cgcttcttta tctatttaaa
     5881 ccttttcatt tttttcatgt tgctattggt gatggctgac aatctgctgc tgctgtttgt
     5941 cggttgggaa ggcgtgggtt tgtgctcgta tctgctgatc gggttttggt accaggatcg
     6001 gcaaaacgtg atcgccggca acaaagcgtt catcgtcaac cgcatcggcg acttcggctt
     6061 cataataggc atttttctcc tggttacgga attgggcagg caggggattt ggacgctcga
     6121 tttcaccgag ctgcagaaga atgtccagat cattggaccg gccaagatca ctctgatcac
     6181 cctgcttctt tccgtcggtg ccgccggcaa atcggcgcaa atcccgctat tcgtttggct
     6241 acccgatgcc atgctcggcc cgactccggt aagcgcgctg atccacgcgg cgactatggt
     6301 aaccgccggg gtctacatga tcgctcgttt gcacttcttg ttcatcctgg cgccttatac
     6361 cctaccgatc attgccgtgg tcggtgcggc cacggccttc ttcgccgcaa ccgtggcgct
     6421 cgcccaaaat gacatcaaaa aagtcctcgc ctattccacc gtcagtcaac tcggttacat
     6481 gtttttggct gtgggcatcg gcgcatttag cgcggcgatt ttccatgtgt tcacccacgc
     6541 cttcttcaaa gcctgtctct tcttaggctc cggcagtgtg atccacgcgc tcgacggcga
     6601 gcaagacatg cgtaagatgg gcggtttgag aaacgcctta cctcagactt attggaccta
     6661 tttgatcgcc actctggcga ttgcggggat accgtttacg gcgggttttt tttccaagga
     6721 cctaattctc tggaaggcat ttgaagatgg gtcgtacggt ttatggtcca tcggcatcat
     6781 cactgccggg ttgactgctt tctatatgtt ccggcaactg ttcatggttt tccatggcaa
     6841 ttttagaggc gacgatcata ccaaagcgca tatccatgaa tcgccgccgc ttatgactgt
     6901 gcctctggtg atcctcgccg cgggctcgat ctttagcggc tggctgggcg cgccggaata
     6961 tctatggggt agtgtctggg acgactggct gcaacccgtc ttcggcgccg gcgccggcca
     7021 ctccgtggag catgctccgg ttagcacgga actgttggtc actgccatta cgttggcagt
     7081 ggtcgcgctg ggcatttacc tggcttacgc ccgatacggc cgcgcccgag caaccgtgcc
     7141 cgcggccgcc gacacaggac tctatcgtct gttgctcgat agttacggca tcgatgaaat
     7201 ttatgatcgg ctggtggtgc ggccgtatac agccgcctcg gattggttgg cgcgagtttt
     7261 tgatcctgtc gtgatcgacg gcgtgatcaa cggcgtggcc agagtgacaa gaggctttag
     7321 ccttatctgg cgtgaagttc agaccggcaa tatccagcac tatctaatcg gtttcctcgc
     7381 tggaaccttg gcgcttcttt attactacct acggcagcaa tagctatgtc acgattgccg
     7441 ggcgatatca gtttactcct cctactgcct ttgattggcg ctttggtgct actactgatt
     7501 ccgcgccagc agcggcaaac cctcttcact ctggccctga ttgcgagtat cgccgctttt
     7561 gtctggtcgt tgcgcctgtt tcaggctttc gatgccggcc caggccagat gcagttcgtc
     7621 gagaaaatcc catggatgcc ggccttcggc attggctata tcgtcggcat cgacggcata
     7681 agtctgtttc tagttttgct gacgaccttt ctcatgccct tggcgatcgc cgcatcctgg
     7741 acggtgcagg acagagtcaa ggagtacctt ttcttcatgc tggtcttgga aaccggcatg
     7801 ctcggcgcgt tcgtcgcgct cgatcttttt cttttttatg tgttttggga agtcatgctg
     7861 gtgccgatgt actttctcat cggcgtatgg ggcggcacgc gccggatcta tgccgcgctg
     7921 aagttcgttg tttacaccat ggccggcagc ttgcccatgc tggtggcgat tatctacctg
     7981 gcgagccgat atgcccatgt gaatcaggcg atgactttcg acgtaatgca gctctacagt
     8041 ttgcgcttgc ctgtcaacga gcaaatctgg ctctttctgg cgtttggctt atcgtttgcc
     8101 atcaaagtgc cgctctttcc atttcacacc tggcttccgg acgcccatgt cgaggcgccg
     8161 acggcgggct cggtgatcct cgcgggcgtg ttgctgaaga tgggcaccta cgggtttctg
     8221 cgctttgcca taccgttatt ccccgacgcg gcggcggttt tcaccccgct gattatcgcg
     8281 ctggcggtaa tcggcatcat ctacggcgcc tgtgtggcga tgatgcaacc ggatctgaag
     8341 aaactggtgg cttactcctc ggtcagccac ttgggattcg tcatgctggg gctcttcgct
     8401 ctcaacatcc aaggcattca gggtggcatc taccagatgg taagtcatgg cttgtctact
     8461 ggagcgctgt ttctcttggt cggcatgctc tacgaccggc gccatacacg tatgatcagt
     8521 gatttcggcg ggctgtggca ccaactgccg ctactctcag tcgcatttct gattgtcacc
     8581 ttcgcctcca tcggcttgcc cgggttgaac ggcttcatcg gcgaatttct aattttactg
     8641 ggagctttca gcctcacacc gagatgggcc gcggctgcga cgaccggcgt cattctcgga
     8701 gccatttaca tgctctggat gtttcggcgg gtatttttcg gaccggtcac ccatcctgag
     8761 aaccagaagc tggtcgatct gaatagtcgt gagctgctga ttctcgctcc tgtcattgcc
     8821 ctgatcatca tcatgggggt gtatccgcaa ccttttctga gccggatgaa aacttccgtc
     8881 gatgtcagct tgcaaagaat tcataattcc aaagcagcgc ccgtcgctac caaccatgcc
     8941 gccgacagag agataaacac caatggacgt taatctcgtg ccgcttttgc cggctgcgca
     9001 agtcttactg gccgctttag tggtcatgct gcttgatctt tttttcgccg accacgaaaa
     9061 gccgcttttg gcagggatca ctgttttcgg cctggcgctg tgcgcggtcg aggccgtgct
     9121 gctttggggc agccgcgaag gagccttcgg agagaccttt ctgttggaca atttcagctt
     9181 gttttttact cagctatttg tcggcgccgc gatcttgacg gttctgtcct cggttcgata
     9241 cgtcagggaa gccggcattc accaaggcga attttactcg ctgattctct ttgcgaccgc
     9301 cggcatgatc gtgatggcgg cggccaatga cctgatcgtt ttttttctgg gtttggaaac
     9361 catgtcggtc gcggtgtacg tgctgaccgg catctggcgt agcagctctc gctctgcgga
     9421 agcggccatg aagtattttc tgatgggctc tttcgccacc ggctttctgc tttatggtat
     9481 cgcgttgatc tacggcgcca ccgggacgac caatttaacc caagtatccg gctacctgtt
     9541 agagccccaa gaacagtggc cgcgatatct ttcggtgggc ggtttgctgc ttctggtggg
     9601 tttcgcattc aaagtgggcg cggtgccgtt tcacttttgg atccccgacg tttacgaagg
     9661 agcgccgacg ccggtgacgg gctttatgtc agtggccgta aaagcggcgg cgtttgccgc
     9721 gtgggcgcgc attctgatgc atcagctatc acccttggac agcgactggg cttatgctgt
     9781 gcgcgtgctt gccattgcca ccatgacttt cggcaatctg ctggcgatct cgcaatcaag
     9841 cgtcaagcgc atgctggctt attcgagcat cgcccatgcg ggatatatcc tgatcggcat
     9901 tgtcgccggc gaggagtggg gcagcacgtc cctcttgttt tatctcgcca cgtatgtgcc
     9961 ggtgactttt ggcgcgtttg ccgtgatcac cgcattgact gaaaaggacc ggccgaaaga
    10021 agagtacgga cactttgcgg gtctaggttt caagcgaccg ttcttagcga tggcgatgac
    10081 attgttcatg ctctcgct
//
LOCUS       DASUVS010000386         4507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_387139, whole genome shotgun sequence.
ACCESSION   DASUVS010000386 DASUVS010000000
VERSION     DASUVS010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387139"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VF182_20655"
     CDS             <1..504
                     /locus_tag="VF182_20655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX6439539.1"
                     /translation="ADIDKKGVIWASLASGHLGSFDRSKCKGPLNGPKATGDHCPEGW
                     SFYKYPGPGFQGIGDNSAESSYYSWVDQHNTFGLGEDVPMSTANLQHGLVAMKDGKMI
                     LLTVPYPMGFYAKGFDGRIDDPKTGWKGRGLWSSSGDRTPWLMENGKGSKPLAVHFQL
                     RPDPLAK"
     gene            815..1813
                     /locus_tag="VF182_20660"
     CDS             815..1813
                     /locus_tag="VF182_20660"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439540.1"
                     /translation="MKIRYGASILILLLFHPAQALPADGPKLQKLVIGYAAPVASLAM
                     IDVMKKGGLFNKHGLDVELVFIPGSGVLTAAMVSGQVPLTFLAGAATVTSAIGGADTV
                     LLSCAINTLFWRLFSVPEIKTVADLKGKRIGVTRLGTLEDGILRYVLKERGLNPERDV
                     SFIAVGPAPQRVAALSKGIIEAASFIPPQDIAAEKLGMNELIDMAKLGLYNPASCFAS
                     TKSYIRSNRDTVLRVMKAFVEGVKFYQDNKDFSLKVIAEFTQNKDLEVLKPSYETVVR
                     FQEKVPHVNMKGIDFILKVIELRDSRAKNFDATLVVDPSFMQELEKTGFVNSVWRN"
     gene            1831..3351
                     /locus_tag="VF182_20665"
     CDS             1831..3351
                     /locus_tag="VF182_20665"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX6439541.1"
                     /translation="MGDALLRNAQRFPTKLALDDGRVRRTYLDLQLRTNGLGNYFLQQ
                     GICPGDHVAVSCGNRAEHLEVIFALAKIGVIAVPFDYHWSRQESDAMLKFFAPKAFVI
                     EERHETADTADLVLDRLGAKRVLFIGETLTPQAKPYEEAISTASPGNNGIEVDGKQPF
                     IIMITSGTTGFPKGCVINHETYALRSLNNAISKGLNAKERGLITLPLQFNAGRQSAMT
                     LLYLGGTIFLRDKFDEGSFLSTIERERITYTIIVPAIGERLLRYPTLDHFDKSTLSFV
                     GISAGHLSPALAGAMMERISLQIYEAYASTDCGQITIIEPEDRPGHGDSVGKPIWAVL
                     LKILDEHDREVSSGPPGEIFVRTPMAIAGYYRNPEATNAFFSDGWCRTGDIGYLDDQG
                     YLHVSGRKKSMIKSAGISIFPEEIECVLSNHADVAEVVVVACRHPQWGESVKALVIPK
                     AGAIVEPDRLMQYCKDLLAPYKAPKIVEVVCSLPRTALGKIDRGKLESAESETTGR"
     gene            3358..4221
                     /locus_tag="VF182_20670"
     CDS             3358..4221
                     /locus_tag="VF182_20670"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6439542.1"
                     /translation="MPGAIDIVINLHTPEVLPLKPKWRKDFIGGKIGADAEILSGLPI
                     EKYLERMDRAGIQMAFIAASRSGSKGHPSNWQLPYEIVADVVSKYPARFKALAGIDPT
                     EGMEGVRELEHAIRELGFVGAHVYPHWFEMPPHHRKYYPFYAKCVELNIPIQMQVGHC
                     LLYSRERPLRSVGRPITLDTIACDFPELKIIGIHTGWPWVEEMISVAWKHPNVYIGSD
                     AYAPKFWKPEFVQFINSWGQDKVLFGTDFPIIDFERATREINELGLKEDSKQRLLWKN
                     TAHVYGLSEFG"
     gene            4278..>4507
                     /locus_tag="VF182_20675"
     CDS             4278..>4507
                     /locus_tag="VF182_20675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439543.1"
                     /translation="MNQSLLIAIDLVFAVSAIPSFAAETQSSAGRTSGWSVKAPLPRT
                     NSEFGVAELDGKIYVMGGYPADRKTVAEVQVY"
BASE COUNT         1092 a         1139 c         1222 g         1054 t
ORIGIN      
        1 gccgatatcg acaagaaagg tgtgatttgg gcgtcgctcg caagcggtca ccttggcagt
       61 ttcgaccgca gcaagtgcaa gggtccgctc aacggtccga aagcgaccgg cgatcactgc
      121 cccgaaggct ggtcattcta caagtatcct ggccctggct ttcagggcat cggcgacaac
      181 agcgctgagt cgagctacta ctcctgggtc gatcagcaca acacctttgg actcggcgaa
      241 gacgtgccga tgtccaccgc caatttgcag cacgggttgg tggcgatgaa ggacggcaag
      301 atgatcctgt taaccgtgcc gtatccgatg gggttttacg caaagggctt cgacgggcgc
      361 atcgatgatc ctaaaaccgg ttggaaaggg cgtggcttgt ggagctcgag cggcgaccgc
      421 acgccatggc taatggagaa cggcaagggg tccaagccgc tagcggtgca ttttcagctt
      481 cgacccgatc cgttggccaa gtagaaaggc cgagtgcgca caataccggc agggcaatcc
      541 caatgagccc attttggctc tggcatcgtg acggatcctt ggctaagtaa atttaatgta
      601 cgagtaaggc tcgggtagac tattgggcag atctaaacgt gttcaataat ttgtcaacct
      661 gagtcttcac aagcacagtc tcggcgtacg ctctcatctt cgatttgaag tgccggtgga
      721 tttttgcgtt cgatcagcaa attccgctga ataccgaccg acatcttctc tttcatatct
      781 cataagtcac atgttcattc agaacggagt gtccatgaag ataagatacg gcgcctcaat
      841 attgatcctg ctattgtttc atccagctca ggccttaccc gccgatggac caaagctgca
      901 gaagctggtg atcggctatg ctgcgcctgt cgcttcgttg gccatgatcg atgtgatgaa
      961 gaaaggagga ctttttaaca aacacggtct cgacgtcgag ctcgtattca ttccgggaag
     1021 cggagtgctg acagctgcca tggtatccgg gcaagttccg ctgacatttc tcgccggagc
     1081 tgccactgtc acttcggcga taggaggggc ggatactgtt cttctttcgt gcgccattaa
     1141 cacactattc tggcggctct tttccgttcc ggagatcaag acagtggccg atctcaaggg
     1201 gaaaagaatc ggcgtgacgc gactgggaac cctggaggac ggtattctgc gctatgtttt
     1261 aaaagagcgc gggctaaacc ctgaacgaga tgtcagcttc attgcggtag gcccggcccc
     1321 tcaaagggtg gcggcactga gcaaggggat tatcgaggca gcatccttta taccaccaca
     1381 ggacatcgca gccgaaaagc tcggcatgaa cgaacttatc gatatggcga aattgggtct
     1441 ttataatccg gccagctgtt ttgccagcac taaatcatac atccgcagca accgagatac
     1501 ggtgttgcgg gttatgaaag cattcgtcga aggtgtcaag ttttatcagg acaataaaga
     1561 tttttccttg aaggtcatcg cagagttcac tcagaacaaa gatcttgagg tgttgaagcc
     1621 ctcttacgaa acggtggtgc gatttcagga gaaggttcct catgtcaaca tgaaaggaat
     1681 cgattttatt ctcaaagtga ttgagctacg agattctcgc gccaagaatt tcgatgccac
     1741 tttggtggtc gatcctagct tcatgcagga gttagaaaaa actggctttg tgaattccgt
     1801 ttggagaaac taaatccggt gaagcttaat atgggagacg cgttactccg gaacgcgcaa
     1861 cgatttccaa ccaagctggc gctggatgat ggcagggtaa ggcgcacgta tttagacctt
     1921 caattgcgaa ccaacggttt gggcaactat tttttacagc agggtatctg tcccggtgac
     1981 cacgtcgcgg tttcctgtgg caatcgcgcc gagcaccttg aagtcatctt tgctctggcg
     2041 aaaatcggcg ttattgcagt tcccttcgat taccattgga gccgccagga atccgatgcg
     2101 atgctcaaat tctttgcgcc aaaagcattt gtcatcgaag aacgacacga gacagcggac
     2161 actgccgatc tggtactcga ccgcctagga gctaaaagag tgttgttcat cggcgaaact
     2221 ttgacaccgc aagctaagcc ttacgaagag gcgatcagca ccgcatcgcc ggggaataac
     2281 ggaatcgaag ttgacggcaa acaacccttc atcatcatga tcacctcggg aaccaccggc
     2341 ttcccaaagg gctgtgtcat taaccatgag acttatgcgc tgcggtctct caacaacgcc
     2401 attagtaagg ggctaaatgc caaggagcgt ggccttatta ctcttcctct tcagttcaac
     2461 gccggccgcc aatcggctat gactcttctt tacctcggcg ggactatttt tcttcgtgat
     2521 aagtttgatg aagggagctt ccttagcacc atcgagcgag aacgaatcac ctatacgatc
     2581 attgtcccgg caataggtga gcgcctcttg cgctatccta cgttggatca tttcgataaa
     2641 tcgactctaa gctttgtcgg aatctctgcc ggacatctct ctcctgcgct ggccggggcg
     2701 atgatggaaa ggatatcgct ccaaatctat gaagcctatg cctcgaccga ctgcggccag
     2761 attacaatta tcgagccaga ggatcggccc ggccacggcg acagcgtcgg taagcctatc
     2821 tgggccgtgc ttctgaaaat cctagatgag cacgaccgcg aagtttcttc gggaccgccg
     2881 ggagaaatct ttgtgcgaac cccgatggca atcgctggct attaccgcaa cccggaggcg
     2941 acgaatgcgt tctttagtga tggctggtgc cgcacaggag atatcggcta tctcgacgat
     3001 caaggttacc ttcatgtctc aggccggaag aagagtatga tcaaaagcgc aggcatcagt
     3061 atttttcccg aggagatcga gtgcgttctt agtaatcatg cggatgttgc agaggtggtc
     3121 gtagtcgcgt gccggcatcc ccaatggggc gaatccgtca aagctctggt aatccccaag
     3181 gcgggtgcca tcgtcgagcc ggaccgtctc atgcaatact gcaaggatct tctggctccc
     3241 tataaggcgc ccaagatagt ggaagttgtt tgcagtcttc cgcgcacagc tctgggaaag
     3301 atcgatagag gcaaactgga atcagccgag tcagaaacca ccgggaggta accgaatatg
     3361 ccgggcgcta tcgacattgt catcaacctg cacacgccgg aggttttgcc cctgaaaccc
     3421 aaatggcgca aagattttat cggcggaaaa atcggtgccg atgcagaaat cttaagcggt
     3481 cttccgatcg aaaaatacct ggagcgcatg gaccgcgcag gcatacagat ggctttcatt
     3541 gccgcgtctc gatcaggcag taaaggacat ccgtcgaact ggcagttgcc gtatgagatc
     3601 gttgcggatg ttgtgagtaa atatccagcg cggttcaagg ctctcgctgg aattgatcct
     3661 acggagggaa tggagggcgt gcgcgagctg gagcacgcta tccgcgaact cggctttgtc
     3721 ggcgctcatg tttatccgca ctggttcgaa atgcctccgc atcatcgaaa gtactacccg
     3781 ttttacgcaa agtgcgtcga gctaaatatt cccatccaga tgcaggtagg acactgcctt
     3841 ctctattcac gagagcggcc gctcagaagt gtcggccggc cgatcaccct ggacaccata
     3901 gcgtgtgatt ttcccgaact gaagatcatc ggaattcata ccggatggcc ctgggtcgag
     3961 gaaatgatct cggtagcatg gaagcatcct aacgtttaca ttggctcgga tgcctatgcg
     4021 cctaaattct ggaagcccga gtttgttcag ttcatcaata gctgggggca agacaaagtt
     4081 ctgttcggaa cggacttccc cattatcgat ttcgaaagag ctactcgcga aatcaatgaa
     4141 ctagggctca aggaggattc gaagcagagg ctgctttgga aaaataccgc tcatgtttat
     4201 ggcttatccg agtttggatg agcaaatatg tttactcggg tgaagacgaa gtacttcgat
     4261 cttgaacagg atctagcatg aaccaaagtt tactaatcgc aatagacttg gtattcgcgg
     4321 tttcagctat cccttcgttc gccgccgaaa cgcagagtag cgcggggcgc acgagcggct
     4381 ggagtgtcaa ggcaccgttg ccgcgcacca actccgaatt tggtgtggcc gaactcgatg
     4441 gcaagattta cgtcatgggt ggctatccgg cggatcgtaa aaccgtcgcc gaagtccaag
     4501 tctacga
//
LOCUS       DASUVS010000387         9074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1479663, whole genome shotgun sequence.
ACCESSION   DASUVS010000387 DASUVS010000000
VERSION     DASUVS010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9074
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479663"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1103)
                     /locus_tag="VF182_20680"
     CDS             complement(108..1103)
                     /locus_tag="VF182_20680"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439544.1"
                     /translation="MNSRLIYLLSFSLITFVVTLPQNLQAKSYIAYISDSTNSSVIYW
                     IAKEAGIFKKHGLDLDTIFINGSVRGIQSLIAGDLSYSGAVGTAVINAKLAGADIAII
                     QSQMNTLPYFIIGNANIKSPEGLKGRSAAVHIPGTSADFAMRLALSKVGIPYSDIKAV
                     TVGGAPARLAAVTNGQMDFTVVTDGERIQGEKMGLKVIIDMAKLNVPFQFNCSVTTRT
                     KIRDNPDEVRRVVWSMAESIHFFKTRKEESIKIMQKYTRGLPRHVLEGAHAANSELLV
                     EDTYPTIDGLKNTLEVQAATDPRAAKFKAEDMVELRFVDEMRKSGFVDKLYSRKK"
     gene            1316..2080
                     /locus_tag="VF182_20685"
     CDS             1316..2080
                     /locus_tag="VF182_20685"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6439545.1"
                     /translation="MGLMNEKVVVVTGGGNGIGRAYCQGIAKEGGAVVVGDINGAAAD
                     GVAKAIADSGGKALSVQVDVASSASCLDMAKVALESFGKIDGLINNAAIFMSVPVTKG
                     GWQEIDEQEWDRVMAVNVKGLWLTSRAVVPAMQQRKQGSIVNISSNMAFNGGLTMMHY
                     VTSKAAVVGFSRVLARELGADNIRVNTLAPGATMSEEKASDAALKNYQRSASTRILKR
                     IEQPEDLVGTALYLLSDLSTFVTGQTILVNGGAVLH"
     gene            2197..3279
                     /locus_tag="VF182_20690"
     CDS             2197..3279
                     /locus_tag="VF182_20690"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6439546.1"
                     /translation="MGWIDADAHVVESPHTWDYLTAAERKFRPMLFKPEGDTQRAHWV
                     IDGKIRGLFRFTFSKEDLEKKSAEIGRDMATTMATRDLADVSARLKHMDELGIDVQVL
                     YPSIFLDQCTERPDTEVALCGAYNRWLADVWKQSEGRLRWMCTLPLLSMPDASDQLNF
                     AKENGACGIFMRPLEGTRQITDPYFFPLYEQAQKIDFGIGLHQANGSAAAVDMMVNPD
                     GSRDFFNQYRIFNVGALFRIMNCGLPKMFPKLRFGFIETAASWVPWVVYELRRRLDTV
                     DSRLPDDILERSGIFVTCQIGDDVPYLIKSCMGENTLMIGTDYGHADSSTELSALSTL
                     KESGGISGEMHRKIVEDNPRKFYGLS"
     gene            3688..3900
                     /locus_tag="VF182_20695"
     CDS             3688..3900
                     /locus_tag="VF182_20695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439547.1"
                     /translation="MRRFLAACLLLLLAGCGDKAKDLYDTAQLEEKQNNQPHATKLYR
                     QIVEEYPDSSYANQAKTRLADLEKAR"
     gene            4669..4950
                     /locus_tag="VF182_20700"
     CDS             4669..4950
                     /locus_tag="VF182_20700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439548.1"
                     /translation="MAVLLITYKCNAATSKADKAFYKVLGSYQWVKLYGSNYAITADE
                     PARDVWQKLKSQTDPDDYLVMLIFKSGTWSLKDQKILAWLVHQPLKIEF"
     gene            complement(5042..5776)
                     /locus_tag="VF182_20705"
     CDS             complement(5042..5776)
                     /locus_tag="VF182_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEX6439549.1"
                     /translation="MAQDKINATRRQTNLPNESHEYLSKREELRGAEIELMRQRERVA
                     ELRRGLPQGAAIQDYAFEEGPASLDSGDTPIRTVRLSELFSAPNRSLVVYQFMLGKKN
                     TTPCPMCTLWIDGWNGVAHHLAQNVDVAIVAAADPMALRAHARARGWHRLRLLSCGAN
                     TFKYDLGSEDREGYQDSTISVFTRDTDGTLRHFYSVHPSLASDIKERGIDLLSPVWHV
                     LDLTPQGRGEWYAELGYGTKVSAAPR"
     gene            6677..7069
                     /locus_tag="VF182_20710"
     CDS             6677..7069
                     /locus_tag="VF182_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6439550.1"
                     /translation="MLRKKIILMVLFSLIAGTLMAQEAKVTPLMSKDLKEFPGKEGVM
                     IAVEYPPGATDPIHRHNAHAFVYVLEGSIVMQLKGGKEVTLTPGQTFYEGPDDVHIVG
                     RSADKTKPAKFVVFLVKDKGAPVLVPVK"
     gene            7173..7994
                     /locus_tag="VF182_20715"
     CDS             7173..7994
                     /locus_tag="VF182_20715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6439551.1"
                     /translation="MSTITSKDGTEIYYKEWGEGPVVTFSHGWPLSSDAWDGQMLFLA
                     QNGYRVVAHDRRGHGRSSQASSGNDMNGYADDLAAIIEALDLRSATLIGHSTGGGEVT
                     RYIGRHGSKRVAKVVLIAAVPPIMLKTPANPEGLPMEVFDGLRAGLVKDRSQFYKDLA
                     IQFYGANRPGAKVSQGTLDQFWLWSMQAGLKNAYESIKAFSETDFTEDLKKIDVPALV
                     LHGEDDQIVPVKDSAKKSAKLIKGAKEIYYPGAPHGITATHQDQVNSDLLEFLRS"
     gene            8124..8879
                     /locus_tag="VF182_20720"
     CDS             8124..8879
                     /locus_tag="VF182_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6439552.1"
                     /translation="MKIVVIGGSGLIGTKLVKLLRERGHEALPASPSSGVNTITGEGL
                     AEALAGAQVVVDLANAPSWEDKAVLEFFETSGRNLLAAEAEAGVRHHIALSIVGTDRT
                     ADNGYFRAKVAQEKLIETSGIPYTIIRSTQFMEFLGGIAASGTDGSTVRISPGLFQPI
                     ASDDVAAIVADVALAAPRNGIVEIAGPERAPFNEIVARYLKGVGDPRVVVSDPEARYW
                     GGRVEEHSLVPLGEARLGRIGLDEWLRRSRTAA"
BASE COUNT         2136 a         2364 c         2512 g         2062 t
ORIGIN      
        1 ccccagatgg tgcgatagaa gagatcaaga tcgtcataat gcgcggcgac cgctgcaaca
       61 tccgctgccg gaacctgagg aaagatcatc ctgtgaccta acctaagtta cttttttcga
      121 ctgtagagct tatcgacaaa gccgctcttt ctcatttcgt caacaaaacg cagctcgacc
      181 atatcttcag ccttgaattt ggcggcacga ggatcggtcg cggcctgaac ctccaaagta
      241 tttttgagcc catcgatagt cggataagta tcttcgacga gcagctcgga attggccgca
      301 tgagctcctt ccaagacatg acgaggtaac ccgcgtgtgt acttctgcat gatcttgatc
      361 gactcttctt tacgcgtttt gaaaaaatgg atcgattccg ccattgacca gacgactcgc
      421 cgcacttcat cgggattgtc gcgaatcttt gtgcgggtgg tcacgctgca gttgaactga
      481 aaagggacgt tcagcttggc catgtcgatg atgactttca gccccatttt ttctccctgg
      541 atccgttcgc cgtcggtgac gacggtgaaa tccatctggc cgttcgtgac cgccgccagc
      601 cgggctggcg ccccgccaac cgtcaccgct ttgatgtccg agtaagggat gccgaccttc
      661 gagagggcaa gtcgcatcgc gaaatccgcc gacgttcctg gaatgtgcac ggccgccgat
      721 cgacccttta aaccttcagg cgacttgatg ttggcattgc cgatgatgaa gtacggcaag
      781 gtattcatct ggctctggat gattgcgata tccgctcccg ccaatttggc gttgatcact
      841 gcggtgccga cggcgccgct ataacttaaa tcgcctgcga tcaaactttg gatgccgcga
      901 acgctgccgt tgataaagat tgtgtcgagg tcgagcccgt gctttttaaa tatgcctgcc
      961 tctttagcga tccagtaaat tacagaagaa ttcgtcgagt ccgagatgta agcgatgtag
     1021 ctttttgcct ggagattctg tggcagcgtg acaacaaaag tgatcaaact gaatgatagg
     1081 agataaatta accggctgtt catcagagaa cctcctggct caattgtttt cctgctgttc
     1141 atatcacaga acccggcaag atgtgttact tcccccggtc atccgctaac gaccgtgttt
     1201 tccggcgtga ttatcgctaa tccgctttcc tgtctataag gttgctggct tcagccgttg
     1261 acacttatca cgaggcggtg atagcgaaag ttctggatca ccagaggtca agaatatggg
     1321 tttaatgaac gaaaaggtcg ttgtggtcac agggggcggc aacggcatcg gccgggctta
     1381 ttgtcagggt atcgctaaag aaggcggggc agttgtggtg ggggatatca acggcgctgc
     1441 cgccgacggg gttgcaaaag cgatcgcgga cagcggcggt aaggcgttga gcgtgcaagt
     1501 cgatgtggca agttcggcaa gctgcctcga tatggcgaag gtagcgctgg aaagcttcgg
     1561 taagatcgat gggctgatca acaatgccgc gattttcatg agtgtgccag taaccaaagg
     1621 cggctggcag gagatcgacg agcaggaatg ggaccgggtg atggcggtca atgtcaaagg
     1681 attatggttg accagccgcg cggtcgtacc ggcgatgcag caacgaaagc agggcagcat
     1741 cgtcaatatt tcctcgaaca tggcgttcaa cggcggactt acgatgatgc attatgtcac
     1801 gtccaaagcg gccgtagttg gtttcagccg ggtgctggcg cgcgagcttg gcgccgacaa
     1861 catccgtgtc aatactctgg cgcccggcgc aaccatgagc gaggaaaaag ccagcgacgc
     1921 ggcgctgaag aattaccagc gttcggcttc gaccaggatt ctcaaacgaa tcgagcagcc
     1981 ggaagatttg gttggaaccg ctctttacct gttatccgat ttaagcacgt ttgtcaccgg
     2041 tcaaacgatc ctggtaaacg gcggagcggt gctgcattga gaagaaggca atgggcagta
     2101 ggcagcaggc aatcgatgga ccgaacgtgc ggaaatctgg ctgtatccga taatccgaag
     2161 tccaaatcgg caatccaaaa tcgctggagg taagccatgg gatggataga cgctgacgct
     2221 catgtggtcg aaagtcccca cacctgggat tacctaacgg ctgctgagag aaagtttcgc
     2281 ccaatgttgt ttaaacccga gggcgacacg caaagagctc actgggtcat cgacggcaaa
     2341 attcgcggct tgtttcgctt cacgttcagc aaagaagatc tagaaaaaaa atccgccgaa
     2401 atcggccgtg atatggcgac caccatggca acccgtgatc tagccgatgt ctcggcgcgg
     2461 ttaaagcaca tggatgagct tggtatcgat gtgcaggttc tgtacccaag catcttcctc
     2521 gatcaatgca cggagaggcc ggataccgaa gtcgctttgt gcggcgctta caatcgttgg
     2581 ctggcggatg tctggaagca gagcgaaggg cgtttgcgct ggatgtgcac gttgccgctg
     2641 ctgagcatgc ccgacgcgtc cgatcaattg aactttgcca aagagaacgg cgcgtgcgga
     2701 atctttatgc ggccgcttga agggacacgg cagatcacgg acccttattt ttttcctttg
     2761 tatgaacagg cgcagaaaat cgattttggc atcggactgc atcaagccaa tggcagcgcc
     2821 gcggcggtcg acatgatggt caatccggat ggtagccgcg attttttcaa ccagtaccgc
     2881 atttttaatg tcggcgcgct atttcgcatt atgaactgtg gacttcctaa gatgtttccc
     2941 aagctgcgtt tcggctttat cgagacggcg gcgtcatggg tgccgtgggt cgtctatgag
     3001 ttgcgccggc gcctggacac ggtcgatagc aggttgccgg atgatatttt ggaacgctcg
     3061 gggattttcg taacctgtca gatcggcgac gacgtgccct atctgatcaa aagttgcatg
     3121 ggcgagaata ccctgatgat cggcaccgac tacggccacg ccgattcttc gaccgagcta
     3181 tcggcattgt ctacactgaa ggaaagcggc ggcatcagcg gcgaaatgca taggaaaata
     3241 gtcgaggaca acccgcggaa attttacggc ttgtcgtgaa gcagaatact tattggtatc
     3301 gatggaagtc tcatcggaat cccacagcgt cagtcactga cgaccgaggt atctcgctgt
     3361 gccttttggg gaacaaagct agaacggaaa catcaagatt caagaccgta gccgagcgcc
     3421 cagcgttgcc gttaaaacta ttagaacacg tagcaaccgg cagcatggtc tcggcgccat
     3481 gagatcgtgg cttatctgtg agtgcgcaat tgtgcgacag tcagccgacg cctctaaacc
     3541 cccgcaacct tgaggattgt caatcaggat gcgttaccta ctcgtcttgg cctgtttgtg
     3601 gattgttcct gctgacactc gcgcagagat gtagtccctt ttctcggctt tggccgcggc
     3661 tgacaccagc aatttaggag aaatttgatg cgaagatttc ttgccgcttg cctgcttctg
     3721 ctgctcgcgg gctgcggcga taaagccaag gatctatacg ataccgcgca gctcgaagag
     3781 aagcagaaca accaaccgca cgcgacgaaa ctttatcggc agatcgtaga agaatatccc
     3841 gactcgtcct acgcgaatca ggcgaagact cgtttggccg atttggagaa agcgcgctag
     3901 acattcatca aggatcctgc acgcctcgct taagcaaaca atctaagcct ggcattatgc
     3961 taagcctgac attatggcgc gtgcgttttg ctgaagactc gtaatctcgc tcactcgcta
     4021 agtcttcatc tgtagcgcga tggcgtacgt attctttttt gtgttcttat attggcttca
     4081 acatcattcc agcgatttcc ttcgtttggc aggctggaaa gctcagcttc ccaactttgc
     4141 cctgctgctg cgttcaaacg ttcttgagcg tcaacaagga actcgtaggg cggggagacc
     4201 aaaaaagagc tgttgataac cgacatctat cagtgggttg aagggtgaaa aaatgacaaa
     4261 aagaccaaag attgccggtg ctccggaagc tcgtccattg catttcagaa atacggaacc
     4321 gttttgcgaa aagcttagaa tttgcaggac aaagcgtacg gcagagtgga aatggagaaa
     4381 ctgcctgtac gatcaccgga aatttagttg aatttttagg caatttggtc aacgccgctt
     4441 ctacttaggc tctgaggcgt gacaggcgaa gtctatccac agggttatcc acattttcgt
     4501 ggaaagtgtc cgcagatcgt gcaaccatcc aaggcccaac atctacacgg taccgacccg
     4561 ccgagcgatc ataccccgca cccgcaatga cgaagtaacc gactagcgcc tcagccttgc
     4621 cgcatttttt ttccaacgtc tgtgcgtcgt gtgctatggt atacgccaat ggctgttctg
     4681 ctcatcacct ataagtgtaa tgcggccacg tccaaagcag ataaggcttt ctacaaagtc
     4741 ctcggaagct atcaatgggt gaagctatac ggatcaaatt acgcgatcac tgcagatgag
     4801 ccggcgagag atgtctggca gaagcttaaa tctcaaactg atcctgacga ttatctggtg
     4861 atgttgatct tcaagtcggg tacctggagc ttaaaagatc agaagattct tgcttggcta
     4921 gtgcatcagc ccttgaagat cgaattttga aaatctgacg attcgataga gatcagcatg
     4981 cgcaggcatg acatcccctg cctgtgtctt tccaccaaga gatgtacgtg cttcgacgac
     5041 atcaacgagg cgccgcactc accttcgtgc cgtaaccaag ctccgcgtac cactcaccgc
     5101 gcccctgtgg agtgagatcc aagacatgcc aaaccggtga cagcagatcg ataccgcgct
     5161 ctttgatgtc actcgccaga gacggatgaa ctgagtagaa gtggcgaagg gtcccgtcag
     5221 tgtctcgtgt gaaaacggaa atggtcgaat cctgatatcc ctcgcggtct tcgctgccga
     5281 gatcgtattt gaacgtgtta gcgccgcaac tcagtagcct gagcctgtgc cacccgcgcg
     5341 cgcgtgcatg tgctcgcaac gccatcggat cggccgcggc cacgatcgca acgtcgacgt
     5401 tctgagccag atgatgcgcg acaccattcc aaccatcgat ccagagcgtg cacattgggc
     5461 acggcgtggt gttcttcttt ccgagcataa actgatatac gaccaaggaa cgattgggcg
     5521 cgctgaacag ttcgctcagg cggacggtgc ggatcggcgt gtcgccggaa tcgaggctcg
     5581 ccggaccctc ttcgaatgca taatcctgaa tcgccgcgcc ttgcggaagc ccgcggcgaa
     5641 gttctgctac tcgctctcgc tggcgcatca gctcgatttc cgcccctcga agctcttctc
     5701 gcttggacag atattcgtgg gattcgttgg gcaaattggt ttgtcgacgc gtcgcattga
     5761 ttttgtcctg tgccatggct catacctcct taagcttttc atcaaaatag ttttggagac
     5821 attgcagctg cattcgaccg ccagacaccg aatgccgcac tcaatagtgc gacgaatgag
     5881 tgagtgcttt taggacagtc gtgctgagat ttttccgacg gttattaaac tatgtcagat
     5941 tgaatggaat ctgaagtatg aatcaaaaga accttgcgac aacttctcgc tccctggctt
     6001 ggaaaaccca atgataccga cgtcgatagt gggggaactt cagtaggaat ggcttttggt
     6061 agtagggatg agtgatattg taaattcaac cagcgagaaa atgacggtta gctctcgacg
     6121 atcgttttcc tcaggaacaa ctttttcggc ctagaactgg cgtctactcg atgactatca
     6181 tagttgtact cgttgtcatc cactcgggcc gacgatgatg aagctgttcg ccggcgagct
     6241 cattcagggc gtcgcgcagc tcctaacgag tcctcagggg atcggaagtg gcaattcgct
     6301 atttcccgac tgctctcaac cggaaaatta acttgacgca tctcgatttt gttatagcgt
     6361 ggggctagaa ctgcattact gctcatcgcg actgtacgtg ccactccacg gagggaacac
     6421 gccgatgaag ccgtcgagag tcttccgatt atccagaatg ccgagcaatt ttcatttcgt
     6481 cgagctcgcg caactcatga tccctaccac gtcgcctccc ggttagacgt tcctattttt
     6541 cagatcatta gcaatattca agaggtgcgc ctcttcagtc cgctcaatca gcggagttaa
     6601 tagggtgcgg agctatccgg tcctggctag taggacccga tcggcacacg tgctcggaca
     6661 aacaacgagg ttaaatatgc tgaggaagaa aatcatactt atggttctgt tctctctcat
     6721 tgcgggaacg ctgatggctc aggaggctaa ggtaacgcct ctcatgtcga aagatctgaa
     6781 agagttccct ggtaaggaag gcgtgatgat cgcagtagag tatccgcccg gagctacaga
     6841 ccccatccat cgtcacaatg cacacgcatt tgtatacgtg ctggagggct ccatcgtcat
     6901 gcagctgaaa ggcggaaaag aagtgacact gacaccggga cagacctttt atgaaggccc
     6961 cgacgatgtt catatcgttg gccggagcgc agacaagacc aagccggcaa aattcgttgt
     7021 gttcttggtc aaggacaagg gcgcgccggt gctcgtgcca gtgaagtgac cgggggagcg
     7081 tcttctccta ttgcggttat aagctgtgac gcgacgctat caaccgctct gaagttcaac
     7141 aaacagatat ttcgagatta aggaggataa ccatgtctac catcacgagc aaagatggca
     7201 cggaaatcta ttacaaggaa tggggcgaag gcccggtcgt aacgttctcg cacggatggc
     7261 ccttgagctc cgatgcttgg gacggccaaa tgctgtttct cgcgcagaac ggatatcgag
     7321 tcgtcgcgca tgaccgtcgt ggtcatggcc gatcaagcca ggcctcctcg ggaaatgaca
     7381 tgaatggtta tgccgatgat cttgcggcca ttatcgaagc gcttgatctg cgcagcgcca
     7441 ctctgatcgg tcactcgaca ggaggcggag aagtcacgcg atatatcggt cgtcatggat
     7501 cgaagcgtgt cgccaaagtg gttctgatcg ctgcagtgcc gccgatcatg ctaaagaccc
     7561 cagccaatcc agaggggctc cccatggaag ttttcgatgg tttgcgcgcc ggcctagtca
     7621 aggatcgctc tcaattctac aaggacttgg ccattcagtt ctatggcgct aaccggcccg
     7681 gcgcaaaagt ctcccagggc acgctggatc aattctggct ttggagcatg caagcaggtc
     7741 tgaagaacgc gtatgaatcg atcaaggcat tctccgaaac cgacttcact gaggatttga
     7801 agaagatcga tgttcccgca ttggtgttgc acggcgaaga tgaccagatc gttcctgtta
     7861 aggattcggc aaaaaagtcg gcgaaactga taaaaggcgc aaaggaaatt tattatcccg
     7921 gagcgccgca cggaataacg gctacccatc aggatcaggt caactctgac cttttggaat
     7981 ttcttcggtc ttgagaggag gtccggtgga accgcaggtt tcgacaggca aattcacagc
     8041 taacgatgga atggaatccg agtaaagggt gcacttaaac actcgtcgga ttgcgcgacg
     8101 cgactacaga aaggagagcg catatgaaga tcgtagtcat aggcggcagc ggcttgatcg
     8161 gcacgaaact cgtgaagctg cttcgtgaaa ggggacatga ggcgttgcct gcatcaccct
     8221 cttcgggcgt caacaccatc accggtgagg gattggcgga agcgcttgcc ggcgctcagg
     8281 ttgtcgtcga cttggcgaat gcgccttcat gggaggacaa ggcggtattg gaattcttcg
     8341 agacctccgg ccgcaacctt ctcgcggcgg aggccgaagc cggcgttcgc caccatatcg
     8401 cgctctccat cgtcggaacc gaccggacgg ccgacaacgg ctatttccgc gccaaggtcg
     8461 cccaggagaa actgatcgag acctccggta tcccgtacac cattatccgc tcgacccagt
     8521 tcatggaatt tctcggcggc atcgccgctt caggcacgga tggaagcacg gtcaggattt
     8581 cgcccggcct gtttcagccg atcgcttcgg acgatgttgc tgccattgtc gccgatgtgg
     8641 cgctcgcagc gccgcgaaat ggcatcgtcg agatcgccgg cccggaacga gcaccgttca
     8701 acgagatcgt cgcccgctat ctgaaggggg tcggcgaccc gcgtgtggtc gtgagcgacc
     8761 ccgaggcccg atactggggc ggccgggtcg aggagcactc gctcgtgccg ttaggcgagg
     8821 cgcgcctcgg ccgcatcggc cttgacgagt ggctccgccg ctcacggacg gcagcctgac
     8881 ccgcattgga ggtacgccca gggagcctca ccccccgggc acggagctaa tccaaagcga
     8941 cgaacgaagg aggcgactat gacaatcaaa ctcgttgcat tgctcctgct atgcctcata
     9001 agcggcacgg cgatcgctca ggagcctagg gtcacgtcgc tcatgtctaa agatcttaca
     9061 gagaatccca gcag
//
LOCUS       DASUVS010000388        13797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1700635, whole genome shotgun sequence.
ACCESSION   DASUVS010000388 DASUVS010000000
VERSION     DASUVS010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13797
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700635"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VF182_20725"
     CDS             complement(<1..418)
                     /locus_tag="VF182_20725"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HEX6439553.1"
                     /translation="MALLTVITASIRDLHPAYFAMVMATGIVSIAAHLLQMRWLAISL
                     AWLNLAAFAVLWLLTLARLFHHRSRAFADLIDHNRGVGFFTMVAASCVLGTQCLLVLE
                     NAPLARVLWCLGIVLWIALTYTIFTALTVKENKPALA"
     gene            complement(750..974)
                     /locus_tag="VF182_20730"
     CDS             complement(750..974)
                     /locus_tag="VF182_20730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6755 family protein"
                     /protein_id="HEX6439554.1"
                     /translation="MNDPRFARHQKKTILSGVLCFVMILVILQLWLLTASMNAYLGGD
                     ESVLVPAALASIACLGLNLGLLWYVYALDR"
     gene            complement(971..1519)
                     /locus_tag="VF182_20735"
     CDS             complement(971..1519)
                     /locus_tag="VF182_20735"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX6439555.1"
                     /translation="MNKRDHDRLTVAPDGRPEADQPRWRQDFPVDWPQDEYIARRDFT
                     KFMVLTSFAFAVGQLWILTRDIFRRSRRASPAREIAAVDEIPVGGSRIFNYPGEHEPA
                     LLVRLDESRFVAYDQRCTHLVCPVIPQIEAGRFHCPCHEGSFDLVSGRPLAGPPRRPL
                     KRVRLEIRAGRVFAIGIEERTA"
     gene            complement(1516..2166)
                     /locus_tag="VF182_20740"
     CDS             complement(1516..2166)
                     /locus_tag="VF182_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6439556.1"
                     /translation="MPVPTQMEFFVDPGRCIGCQSCVQACSECDTHKGHSMIQLDYID
                     RASSTQTVPVICMHCDSPTCAEVCPADAIKRTGDGVVQSARKPRCIACNNCVIACPFG
                     VPKMNTGMHLMMKCDMCYDRTSVGKKPMCASVCPSQALFFGTRDEIEKLRPLSQPINQ
                     FQFGNQTISTRVNLMVPKAARIEHLDVTAALHEPPVGQDVLADLLMDNLTADGEEG"
     gene            complement(2180..4426)
                     /locus_tag="VF182_20745"
     CDS             complement(2180..4426)
                     /locus_tag="VF182_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX6439557.1"
                     /translation="MATIPVNTIEVIERFGPHLARSKGARLDPGVEPDKTVKTHCCFC
                     GQQCGIELLVKNNEVIGFEPWMDFPFNQGKLCPKGVKRYLQGSHPDRLLHAHERDPSA
                     PAGFKPIEYRRAIQCVAEAIGRIQSQYDNHAFAVLTGASLTTEKAYLMGKFAHMCLKT
                     SNIDYNGRLCMVSAAAGNKKAFGIDRAANPWSDILDAQVVWISGANVADCAPITTDYV
                     WQARENGAKIIVVDPRITPVARTCDLFLPIKPGRDVALFNGILHLMIENDWLDHDFIE
                     RHTAGFDEVASHVAEWTPRKTAEVTGIAERGVRQAAEWWGKAKTSFLMHARGIEHHTH
                     GVQNVLGAINMVLASGRLGRNGCGYATITGQGNGQGGREHGQKCDQLPGARDISNSED
                     RATIAKVWGVEADEIPQAGVDAYEIFRKIDAGEIKGLLTICFNPVVSLPDNNFIRRML
                     EKLDFYVAIDFFLNETARYADVVLPGSLQEEDEGVVTTAEGRVIKINKAVDCPGEARE
                     DWRIIQDIAGALGRERGFTFNSPGEIFAELRQASRGGIADYSGITYEKIERQYGIFWP
                     CPSEDHPGTPRLFEEKSWNPVARGNGRFYFPDGKARFNVARYTPPAEDVDDEYPIILT
                     TGRVVSQFLSGTQTRRIGPLVDHYPEPRVEIHPILAETLEIGDGEWITVESRRGDCTL
                     RAQLVKTIRPDTVFIPYHWAGPRSVNQLTISAQDPISKIPEFKVCAVRLKKAAREPEY
                     ANQLEPQQ"
     gene            complement(4433..5620)
                     /locus_tag="VF182_20750"
     CDS             complement(4433..5620)
                     /locus_tag="VF182_20750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6439558.1"
                     /translation="MAAASTKKTSFIKLEVIKAAAWGIGATLILAVLIVVGSRNLSQF
                     DAALVGYTFATLFAVFGITYRYSMWLQRPPTALYWRRGWQAYFKREQRLVNFKNWVQR
                     VVNDIGLNRFILRRGRHRWLAHWLIMWGTIIAAGITFPLVFGWIHFQSVAGEIDRYRL
                     FLFGFPTFTFSVHSTLAFFLFHGLVWSAFLVIAGVMIAMRRRMREQGAAAVQLFTEDL
                     LPLILLFAISVTGLLLTASYTWMKGYAFDFLAIVHAITVIFTLLWLPFGKLFHIFQRP
                     AQLGVGFYKDVGSRQEHALCRRCGHPFSSRLHVEDLIGVERELGYRYEMEGNDAQHYQ
                     WICPPCRRAMLAIAQGKIWQQARGAQPPSKLQTQAAAMPSYANPVIGEGPLGEEDQNN
                     FHP"
     gene            5778..6284
                     /locus_tag="VF182_20755"
     CDS             5778..6284
                     /locus_tag="VF182_20755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX6439559.1"
                     /translation="MKQGPVYRFLADDHARLDLLLQRAFADPERIDRHAYSEFRSGLL
                     KHIAMEEKVLLPAAQQARDGEPLPVAAKLRIQHGALAALLVPSPTCAIGAAIRAILKE
                     HNPLEEGSGGVYEVCEALAGAEAVTMLAKLQSYAEIKLAPHNDGPLVMEATRRAIARA
                     GYNLEDYT"
     gene            6388..7779
                     /gene="asnS"
                     /locus_tag="VF182_20760"
     CDS             6388..7779
                     /gene="asnS"
                     /locus_tag="VF182_20760"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEX6439560.1"
                     /translation="MDQQATSVRHAFDLPVGTSLTLRGWVRSRRDSKGVTFIELNDGS
                     RFKSMQLVIDGGGVADEILKQVTTGSSIEAGGVLVQSPAKGQAVELKVSHIHVYGSAD
                     PATYPLQKKGHTLEFLREISHLRVRSNTLGAAFRVRNALTHAIHTFFQERDFIYVQTP
                     IITTSDCEGAGQMFNVTSLNMNKPPHAADGKVDWQQDFFGRPAYLTVSGQLEAEIFAL
                     AFSKVYTFGPTFRAENSNTPRHLAEFWMIEPEIAFYELADNMRLAEEFLKYIIRHVLD
                     HCREDLEFFNERIEKTVLSTLEHVAQSSFGHITYTDAVQELKKSGRAWEFPIDWGHDL
                     QTEHERFLSEEVFKKPVIVTDYPKDIKPFYMRVNDDDRTVRAMDVLVPRVGEIVGGSQ
                     REDRYDVLLERLRASGLDEKAYSWYLDLRRYGSVPHSGFGLGLERIMMYLTGLKNIRD
                     VIPFPRTPGNADF"
     gene            complement(7848..9758)
                     /locus_tag="VF182_20765"
     CDS             complement(7848..9758)
                     /locus_tag="VF182_20765"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6439561.1"
                     /translation="MEENSATRQLGLDPQESAEGKELPEHRSMVTSRISRTAGKVNPF
                     TIAATVLVIVAFYWAQAVLIPFALALLLTFLLSPLVTRIQQAGLGRGPSVTIIIALTF
                     AVLAMIGWATVSQVTSLAEELPAYRHNIRQKIRDLRGMGKGGVIEQVQETVDEVKDEL
                     EQGQAPSAANDRRPIIVQAESAVSWRSLYLGPLIQPIASATLVLGLLIFMLAQREDLR
                     NRLIRIIGYAHLTVTTKALEEAGQRISRYLLMQITINGCFGIIISFALFLIGLPYAFL
                     WGFLSVPLLFIPVIGFWTAAALPTIMSMGVFTDWWWPLVVVGLFLVLKTIINMLLEPL
                     LYGRTVGVSPVPLLMMIAFWTWLWGPVGLLLATPLTVCLVVFAKNVPQLEFVEVLLSD
                     EPAMEIQVSFYQRLLAMDQVEAVEILKQYLKEHKPENIYDELLMPSLSYAKEDRRRNN
                     LSDREELFLFKAMREIIEIAGDELQRNTISEDGATASFVTDHLPTMKIIGCPAHGEAD
                     EVALLMLRQLLAQKSCSVDILSSAVLAAEVIARVREHNPDLLCIAAVAPDGWVQVRYL
                     SKRLRASFPDLRLVIGRWGLGEFDENGDALSDDPGEVGSTLLQTRDRITNLRQLILDV
                     EPKNRTETLPAS"
     gene            10070..11302
                     /locus_tag="VF182_20770"
     CDS             10070..11302
                     /locus_tag="VF182_20770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6439562.1"
                     /translation="MAPWLRLRPTARGLIILYTSTLLSGMWSMLVPSIPVMAVHFGIS
                     PGTAAQIITALAFGRLAGMPISGVVLDRLGTRAALITGPTLACAVALLAAIVPWFSII
                     LLLLFVLGIAESVWVIAREVAGIDLALPNQRGRVLSGFHGVNYMGLALGPLAGGLLAE
                     TSGFRGAFIAYAACAAISVLLGFTVENSPTPHNFQAVPATESRRITAWAQRLRNVSDL
                     VHQIPPHLRTTYVVLVFATFTSFIHRVTTQSMLPLFATTELGLSPKEVGLLFTISGVA
                     AFAMILPAGFVIDKVGRKWATVPSTGIPALAFLLIPFAGNFFELSLLMLFLGIANGLS
                     LGSLATSTFDVIPVNARGRLQALRRTIAELGGVGAPLAGGVLADSFNPGVPFLVYAPL
                     IILSAVLLAFIGRETLQR"
     gene            complement(11503..12399)
                     /locus_tag="VF182_20775"
     CDS             complement(11503..12399)
                     /locus_tag="VF182_20775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX6439563.1"
                     /translation="MGFKQDLKDTFSLRGVSAMQLIKRVIAEVQEDNCAVYAASLAYY
                     FLFALFPFFLFLTALLAYLPIPNLMDRIIGLLAEVMPGEALSLVEENVGKLVSQQRGG
                     LLSFGIVAALWTSSSALTGISDALNHAYDVEEGRPFWKVRGMAILLTVGLSIFLIASI
                     VLLMFGPQIGGWIADFVGLGRVFEIAWNILRWPVILILVMSAVAAIYYFAPDVDQSWK
                     WMTPGSVFAVVLWILVSLGFSYYVNNFGSYDKTYGSIGAIIVLLTWMYVSGFVILVGG
                     EINAELEHASAEGKAPGKRTGA"
     gene            12737..>13797
                     /gene="aroG"
                     /locus_tag="VF182_20780"
     CDS             12737..>13797
                     /gene="aroG"
                     /locus_tag="VF182_20780"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEX6439564.1"
                     /translation="MEYRTDDLRIKEIKELLPPAQVLRDIAITTKAAETVYQTRQAIH
                     RILHGADDRLLVILGPCSIHDVKAAKEYAGKLKEVKDRLSSDLLVVMRVYFEKPRTTV
                     GWKGLINDPQLDGSFRINEGLRIGRRLLLDLNEMAVPAGSEFLDMITPQYVADLVSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGNVRIAVDAIRAAQAPHHFLSVTKDGHS
                     AIVSTTGNEDCHVILRGGRNPNYDSKNIDAAAKSMAEAGISARIMIDCSHGNSGKEPE
                     KQVEVGQQVSAQIADGDARIFGVMIESHLKAGRQDLIPGKELIYGLSITDACVGWEDT
                     RKVVDHLAHAVRQRRLKAAA"
BASE COUNT         3156 a         3949 c         3763 g         2929 t
ORIGIN      
        1 ccgccagcgc gggcttgttt tccttcaccg tcagggcggt gaaaatcgta taggtcaggg
       61 caatccaaag aacaattccc aagcaccaca aaacacgggc aagcggcgca ttttccagca
      121 cgagcaggca ctgggttccg agaacgcacg atgccgccac catggtgaag aaaccaacac
      181 cccgattgtg gtcgatcaga tccgcaaaag cgcgcgatct gtggtgaaac aggcgtgcca
      241 aggtcaacag ccagagaacc gcgaaggccg cgaggttaag ccaggccagg ctgatggcca
      301 gccatcgcat ctgcagcaga tgcgcggcaa tcgagacgat cccggtggcc atgaccatgg
      361 caaagtatgc cgggtgcagg tccctgatcg atgccgtgat cacggtaaga agagccatga
      421 gctgtgtttc atcctggcga agacgatcga ctgttcagtg gccggaagcg agcgcttgtc
      481 actgccgatt ctcagttccg cagttgcaat cgacgcagca agcgaggagc aacccttcca
      541 taacgaagtc aatggctctc tgcctctgat cagacatcga tcgatcgtgt tcgacgagca
      601 tctgaagctc acggcaacaa ggtgtggaat cgctaaaatg attctcgaaa actcacggtg
      661 agtgccttcg ttactccaga cttgcacctc cccaagtgcc gacaggtttg actgggactg
      721 cgggccgatg gcaagagatg cctcggcact catcgatcca aggcatagac gtaccagagc
      781 aacccgaggt tgagcccgag acaggcgata ctggccaggg ctgccggaac gagaaccgat
      841 tcgtcgccgc cgagataggc attcatcgac gcggtgagca gccacagctg gagaataacc
      901 aggatcatga caaagcacag gacgccggag agaatggttt ttttctgatg gcgcgcgaag
      961 cgtgggtcgt tcatgccgta cgctcctcga tgccgattgc aaaaacccgc cccgcgcgaa
     1021 tctcaagccg gacacgctta agcggccggc gcggagggcc cgccagcggc cgcccgctga
     1081 ccagatcgaa cgagccttca tgacaaggac aatggaagcg cccggcttcg atttgaggaa
     1141 tcaccgggca taccaggtgc gtgcatcttt gatcgtaagc gacgaaccgc gactcatcca
     1201 agcgcaccag cagcgccggc tcgtgctcgc ccgggtaatt gaaaattcgt gaaccgccga
     1261 ccggaatctc gtcgaccgcg gcgatttccc gcgccggcga cgctctcctg ctgcgccgga
     1321 aaatatctcg cgtcaaaatc cacagctgcc ccaccgcgaa agcgaagctt gtcagcacca
     1381 tgaatttggt gaagtcgcgc cgggcgatgt actcgtcctg tggccagtct acgggaaaat
     1441 cctgccgcca tcgcggctgg tctgcttcag gcctgccgtc cggcgccacc gtcagccggt
     1501 cgtgatctcg cttgttcacc cttcctcccc atccgctgtc aggttgtcca tcagcaaatc
     1561 agcgagcaca tcttgaccca ccgggggttc gtgcagcgct gcggtgacat cgagatgctc
     1621 gatcctggca gccttgggta ccatcaggtt gactcgcgtg ctgatcgttt gattgccgaa
     1681 ttgaaactgg ttgatcggct gcgacagagg ccggagcttt tcgatctcgt cgcgggttcc
     1741 aaaaaacaga gcctggctgg gacagaccga ggcgcacatc ggctttttgc ccaccgaggt
     1801 gcggtcatag cacatgtcgc acttcatcat tagatgcatg ccggtgttca tcttgggcac
     1861 gccgaacgga caggcgatca cgcaattatt acaggcgata cagcgcggct tgcgcgcgct
     1921 ttgtaccacg ccgtcaccgg tgcgcttgat cgcatcggcc gggcagactt cggcgcaggt
     1981 cggcgaatcg caatgcatgc agatcaccgg cacggtttgg gtcgagctcg ctcgatcgat
     2041 gtaatccaat tgaatcatcg agtgaccctt gtgcgtgtcg cattcgctgc aagcctgcac
     2101 gcacgattga cagccgatgc agcggcccgg gtcaacgaag aactccatct gtgttggaac
     2161 tggcatggtc gtctctgctc tactgctgcg gctcgagttg gtttgcgtac tccggctcgc
     2221 gcgcggcctt tttcagtctg accgcgcaca ccttgaattc agggattttg gaaattggat
     2281 cctgtgcgga gatcgtcagc tgattaacgc tgcgcggtcc tgcccagtgg taggggataa
     2341 agacggtatc ggggcggatc gtcttgacca gctgcgctct cagagtgcag tcgccgcgtc
     2401 gcgactcgac cgtgatccat tcgccatcgc caatctcgag cgtttcggcc agtatcggat
     2461 gaatttccac gcgcggttcg ggataatgat ccaccagcgg cccaatccgc cgggtctggg
     2521 tgccggaaag aaattgactc acgacccttc cggtcgtgag aatgatcgga tattcatcat
     2581 cgacatcttc ggccggcggc gtataacgcg ccacgttgaa gcgggctttg ccgtccggga
     2641 aataaaatct gccgttgccc cgagcaactg gattccagga tttttcttcg aacagtctgg
     2701 gtgtccccgg atgatcttcg ctcggacaag gccagaaaat tccatattgg cgctcgatct
     2761 tttcataggt aatgccggag taatccgcaa tgccgccgcg cgaagcttgg cgcagttctg
     2821 caaatatttc acccgggctg ttgaacgtaa agccgcgctc tcggccgagc gcgccggcga
     2881 tatcctggat aatgcgccaa tcctcgcgcg cttccccggg acagtcgact gccttgttga
     2941 ttttgatcac ccgtccttcc gcggtggtaa ccacgccttc gtcttcttcc tgcagcgagc
     3001 ccggcagcac aacatcggcg taacgggcgg tctcgtttaa aaagaaatcg atggcgacat
     3061 agaagtcgag cttctccagc atgcgcctga taaaattatt atcgggcaac gagaccaccg
     3121 gattgaaaca aatcgtcaag agtcctttga tctcgccggc gtcgatcttg cgaaagattt
     3181 cataggcatc gacgccggcc tggggaatct cgtccgcctc cacgccccaa accttggcga
     3241 tggtggcccg gtcctccgag ttcgagatat cgcgcgctcc cgggagttga tcgcatttct
     3301 ggccgtgctc gcgcccgccc tggccgttgc cctggcccgt gatcgtggca tagccgcaac
     3361 cgttgcgtcc gagtcggcct gatgccaaca ccatgttgat cgcgccgagc acgttctgca
     3421 caccgtgcgt atgatgttcg atgccgcgcg cgtgcatcag aaagctcgtt ttagctttgc
     3481 cccaccactc ggccgcctgc cggacgcctc gctcggcgat gccggttacc tcggcggttt
     3541 ttcgcggcgt ccattcggct acatggctgg cgacttcgtc gaacccggcg gtgtgccttt
     3601 cgatgaaatc atggtccagc cagtcgtttt cgatcatcag gtgaaggatg ccgttgaaga
     3661 gcgccacgtc gcgccctggt ttgatcggca ggaacagatc gcaagttcgc gcgacgggag
     3721 tgatgcgcgg gtctacgacg ataatcttgg cgccgttttc ccgcgcctgc cacacataat
     3781 cggtggtaat gggggcgcaa tccgctacgt tggcgccgct gatccagact acttgtgcgt
     3841 cgagaatatc cgaccatgga ttggccgctc ggtcgatgcc gaaggctttt ttattgccgg
     3901 cggcggcgct caccatgcac aatcggccgt tgtaatcgat attcgaggtc ttgaggcaca
     3961 tgtgcgcaaa cttgcccatg agataagcct tctcggtagt caggctggca ccagtcagca
     4021 ctgcgaaagc atggttgtca tactgcgatt gaatgcgtcc gattgcctcg gcgacgcatt
     4081 ggattgcgcg gcgatattct atgggcttaa aacccgccgg cgcggatgga tcgcgttcgt
     4141 gagcgtgcaa cagccgatct gggtgggacc cctgaagata gcgtttcacg cctttgggac
     4201 agagcttccc ctggttgaaa ggaaagtcca tccacggctc gaagccgatg acctcattgt
     4261 tcttgactag aagttcgatg ccgcactgtt ggccgcaaaa acagcaatgg gttttcaccg
     4321 ttttgtcggg ttccacgccc gggtccagcc tggcgccctt ggagcgcgcc agatgcggcc
     4381 cgaaacgttc gatgacttca atagtgttaa cgggaatggt cgccatacaa tcttacggat
     4441 gaaaattgtt ctgatcttct tcgccgagcg ggccttcgcc gatgaccgga ttggcatagg
     4501 atggcatcgc tgcagcctga gtctggagct tcgacggcgg ctgtgcgccg cgcgcctgtt
     4561 gccagatttt cccttgagct atggctagca tcgcccgccg gcagggaggg cagatccatt
     4621 gatagtgttg cgcatcgttt ccttccatct catagcggta gcctaactcg cgctcgactc
     4681 caatgagatc ctcgacatgc aggcgcgagc tgaaaggatg accgcagcgt cggcaaagtg
     4741 catgttcctg gcgcgaaccg acatctttat aaaagccgac cccaagctgt gccggtcgct
     4801 ggaagatgtg gaacaacttg ccgaatggca gccataaaag cgtaaagata acggtgatgg
     4861 cgtggacgat agcgaggaaa tcgaacgcat agcctttcat ccaagtgtag ctggcagtca
     4921 acagcaatcc agtgacactg atcgcgaaga gcagaatcag tggcagcagg tcctcggtaa
     4981 ataattgaac cgcggccgcc ccctgttcgc gcatgcgccg gcgcattgcg atcatgacgc
     5041 ccgcgatgac cagaaaggcc gaccacacca ggccgtgaaa cagaaaaaac gccaatgtgg
     5101 agtggaccga aaacgtgaac gtgggaaagc cgaagagaaa cagacgatac cgatcgatct
     5161 caccggcaac agactgaaag tgaatccagc cgaataccag tggaaacgta atgccggcgg
     5221 cgatgatcgt accccacatg atcaaccagt gcgccagcca gcggtgacgg ccgcgacgca
     5281 ggatgaagcg gttcaatccg atgtcgttga cgacgcgctg aacccagttc ttgaagttca
     5341 cgagccgctg ctcgcgcttg aaataagcct gccagcctcg gcgccagtaa agcgccgtcg
     5401 gcggccgctg cagccacatg gaatagcgat aagtaattcc aaaaaccgca aatagcgtgg
     5461 cgaacgtgta gccgaccagg gccgcatcga attgcgacag gttgcgcgat ccgactacaa
     5521 tcagcacggc caggattagc gttgcaccaa tcccccatgc agcagctttg atgacctcga
     5581 gttttatgaa cgatgtcttc ttcgtagaag cggctgccat gtttccttcc aagaaaacac
     5641 tacatcttaa tgatcttcga gtgaagccat cactgtcttt ttacgggacg aatagatttt
     5701 agaaatcaaa tgtctatact taaaattcaa aatcatgaag accaacacgc tggaatctcg
     5761 cggaggcaga aactgaaatg aagcaaggtc ctgtataccg ctttctagcg gacgatcatg
     5821 ctcggctgga tctgctactg cagcgtgcct ttgccgaccc ggagcggatt gatcgacacg
     5881 cttacagtga gtttcgttcc ggcttgctca agcacatcgc aatggaagaa aaagtattgt
     5941 taccggcagc tcagcaggcg cgggatggag aacccctgcc ggttgccgcg aagctgcgca
     6001 tccaacacgg cgcactggca gcgcttcttg ttccttcgcc gacatgcgcg attggcgcgg
     6061 ccattcgcgc aatcctgaaa gagcataatc ctctggagga aggctccggc ggcgtctacg
     6121 aggtttgcga agcattggcg ggcgccgaag ccgttaccat gcttgccaag ctgcagtcgt
     6181 atgccgagat caagctggcc ccgcacaacg atgggccgct ggtcatggag gccacacgcc
     6241 gggctatagc gagagcgggc tacaatctgg aagattacac ttagatgttg cgacagctac
     6301 agctaagttc caaccggaac atacgttggg gggcagtttc acttaccagg ttgacatcga
     6361 gcaagccgat cttcgatact gccgactatg gatcaacaag ctaccagcgt cagacacgcc
     6421 tttgatctgc cggtcggcac gtcgcttact ctgcgcggct gggtgcgttc ccggcgggat
     6481 tccaaaggtg tcaccttcat cgaacttaac gacggctcgc gcttcaaaag catgcaattg
     6541 gtaatcgacg gcggcggcgt tgccgatgag atactgaaac aagtcaccac cggcagcagc
     6601 atcgaggccg gcggcgtgct ggtccaatcg ccggccaaag gccaggcggt ggagctcaag
     6661 gtttcacaca ttcatgtcta cggcagcgcc gatcccgcca cttatccatt gcaaaagaaa
     6721 ggtcacaccc tggagtttct ccgggagatt tcccatctgc gtgttcgcag caacaccctc
     6781 ggcgccgctt ttcgcgtgcg caacgcgctc actcacgcga ttcacacgtt ttttcaggaa
     6841 agagatttca tttacgttca gacaccgatt atcacgacat cggattgcga gggcgcgggg
     6901 cagatgttca atgttacgtc tttgaatatg aacaaaccgc cgcacgcagc cgacgggaaa
     6961 gtggattggc aacaagactt tttcggccgt cccgcgtatc tcacggtcag cggccaactc
     7021 gaagccgaaa ttttcgcgct ggcgttttcc aaagtttata ctttcggccc aacttttcgc
     7081 gccgaaaaca gcaatacgcc gcggcatctc gcggagttct ggatgatcga accggagatc
     7141 gccttctacg agctggcaga caacatgcgc ctggccgagg agtttttgaa atacattatt
     7201 cgccacgtcc tggatcactg ccgcgaagac ttggaatttt tcaacgaacg catcgagaaa
     7261 accgtgttga gcacgctcga acacgtggcg caatccagtt tcggccatat cacctacacc
     7321 gacgcggtac aggagttaaa aaaatccggc cgggcctggg agtttcccat tgactggggt
     7381 catgatctgc aaaccgaaca cgaacgcttt ctttcggaag aagtttttaa gaaaccggtg
     7441 atcgtcacgg attatcccaa agacatcaaa cctttttata tgcgggtcaa cgatgacgac
     7501 cgcaccgtgc gcgccatgga cgtgctggtt ccgcgtgtgg gtgaaatcgt cggtggcagc
     7561 cagcgcgaag atcgttacga cgtcctgctg gaacgtctgc gcgccagcgg tctggatgaa
     7621 aaagcctact cgtggtacct cgatctgcgc agatacggct ccgtcccgca ttcgggcttc
     7681 ggcctcggac tggagcgcat catgatgtat ctcaccggac tgaaaaacat ccgcgacgtg
     7741 attccgtttc cgcgcacgcc gggcaacgcc gatttttgat tatttaatgc gccgcgcgcc
     7801 tcttggacgg tcgatccgtg agacagcaag cttgccgcag caaaatccta gctggcaggc
     7861 aacgtttcgg tgcggttttt tggttcgaca tccaaaatga gctggcgcaa attggtgatc
     7921 cgatcgcgag tttgcagcag agttgatccg acctcgcctg gatcgtcgct gagcgcatcc
     7981 ccgttttcat caaactcacc gagcccccat cggccaatca ccagcctgag atcggggaag
     8041 gatgcgcgca ggcgcttcga tagatagcga acctggaccc atccatcggg ggcgaccgct
     8101 gcaatgcaaa gcaaatctgg attgtgttcc cgcactcggg ctatgacttc agcagccaaa
     8161 acagccgagg acagaatatc cactgaacaa cttttttggg cgagcagctg gcgcagcatc
     8221 aaaagagcga cctcatcggc ctcgccgtgc gccgggcaac cgatgatctt cattgttggc
     8281 aaatggtccg tgacgaatga cgctgttgca ccgtcttccg atattgtatt tcgctgaagc
     8341 tcgtctccgg cgatctcgat aatttcccgc atcgctttga acaaaaagag ttcctcacga
     8401 tcgcttaagt tgttacgccg gcgatcctcc ttggcatagc tcaaagatgg catgagcaac
     8461 tcatcataaa tattttcggg cttatgttcc tttagatact gcttgagaat ctcgactgcc
     8521 tcgacctgat ccatggctaa taaccgctga tagaagctga cttgaatttc cattgcgggc
     8581 tcgtcgctca acagcacttc gacaaactcg agctgcggaa cattttttgc gaaaaccacc
     8641 aggcaaaccg tcaacggcgt tgcaagcaga agtcctaccg gtccccatag ccaggtccaa
     8701 aacgcgatca tcatcaggag tggcaccggc gagactccga cagtgcggcc gtacagcaaa
     8761 ggttctaaca gcatgttgat aatagttttc aagaccagga aaagcccaac cacgaccaac
     8821 ggccaccacc aatcggtgaa gacccccata ctcattatcg tcggtagtgc cgccgccgtc
     8881 caaaatccga tcaccggtat aaacagcaac ggaaccgata agaatcccca gagaaacgcg
     8941 tagggcagac cgatcagaaa aagcgcaaaa ctaatgatga tgccaaagca gccattgatg
     9001 gtgatctgca ttaacaagta acggctgata cgctgtcccg cttcttccaa cgccttggtc
     9061 gtaaccgtca gatgcgcata gccgatgatc cgaatcagcc gattgcgcag gtcttcgcgt
     9121 tgggcaagca taaagatcag caagcccaac accagcgtgg cgctggcgat cggctgaatc
     9181 aaaggtccta aatagagcga gcgccaggaa accgccgatt cagcttgaac gataatcggg
     9241 cggcgatcat tcgccgccga aggagcttga ccctgctcca actcatcctt aacctcatcg
     9301 accgtttcct gtacctgctc gattactccg ccttttccca ttccccgcag atcccggatc
     9361 ttctgacgga tgttatggcg atatgccgga agttcttcgg ctaggcttgt gacttgagaa
     9421 accgtagccc agccgatcat cgccaggacc gcaaaagtca gggcgatgat aatcgtgacc
     9481 gacggtccgc gtccgagacc tgcctgttgg attctggtga ccaatgggct caataaaaag
     9541 gttagtaaaa gcgccaacgc aaacgggatc agcacggctt gcgcccagta gaaagcaaca
     9601 atcaccagga ccgttgcagc aatcgtgaaa gggtttacct tgccggccgt gcgactaata
     9661 cggcttgtta ccatcgaacg atgttccggc aattctttcc cttccgctga ttcctgagga
     9721 tccaaaccaa gctgtctggt cgcgctgttc tcttccatcg tcgtcatctt ttacgtcact
     9781 tatgatccga gctttatacc aagcgccacc cgggctcaat cacatattgc caagctctta
     9841 tccacaatgg atagatccct tcagtagtct atgctaacta atggattctg ttttctgact
     9901 caccacgcta cgcaccgaca atacgaacag cgaagaacgc gttagaaatt ccgccgcgta
     9961 ttatctggtg ctcctgatct ttcatggtgg tacagtttag agacaattga ctcaccgcat
    10021 cggcttccgg ccagaacgcc gtatcggaac gaatcttaaa ttaaaaaaca tggcaccttg
    10081 gctaagactt aggcccacgg cgcgcgggtt gatcatcctc tatacaagca cgctcctatc
    10141 cggaatgtgg tcgatgctgg tgccgagtat tcctgtcatg gcggttcatt tcggcatctc
    10201 tccgggtacc gccgcacaga tcattacggc cttggctttc ggcaggcttg ccggtatgcc
    10261 gatcagcgga gtcgttctcg accgactggg cacgcgcgcc gcgctgatca ccggacccac
    10321 attggcctgc gccgttgcgc ttttggctgc catcgtgccg tggttcagta tcattctgct
    10381 cttgcttttt gtcctcggca tcgccgaaag cgtttgggta attgcgcgcg aggtagcggg
    10441 catcgatttg gcgcttccca atcaacgcgg gcgcgtcctc agcggttttc acggcgtcaa
    10501 ttacatgggg ctggcgttgg gacctttagc gggaggcctc ctggctgaaa ccagcggctt
    10561 tcgcggagca tttattgcct acgccgcttg cgcggcaatt tctgtgctgc ttggcttcac
    10621 ggtcgaaaat tctccaactc cccacaactt tcaggccgtc ccggcgacag agagccggag
    10681 gataactgct tgggcgcagc gattgcgcaa tgtgagcgac cttgtccatc agattccgcc
    10741 acacttgcga acaacttacg ttgttctggt atttgccacc tttaccagtt tcatacaccg
    10801 ggtcacgacg caaagtatgt tgccgctgtt tgcgacaact gagctcggcc tatcacccaa
    10861 agaggtggga ctcttgttca caatctccgg tgtagccgct tttgcaatga ttctccctgc
    10921 gggtttcgtt atcgacaaag tcggtcgcaa gtgggccacg gtgcccagca ccggaattcc
    10981 ggccctggcg tttctgctca taccgttcgc cgggaatttt ttcgagcttt ccctgctgat
    11041 gttatttctc ggcatcgcca acggcctctc gttaggctct ctagcgacct cgacttttga
    11101 cgttatccct gtcaatgctc gagggcgatt acaagcgctt cgacgaacca tcgccgagct
    11161 cggcggcgtc ggcgcgcctt tagcgggagg cgttctcgcc gacagcttca acccaggagt
    11221 gccgtttctc gtctatgctc cgctgatcat tctatcggct gtactgctgg cgtttatcgg
    11281 gcgtgaaaca ctgcagagat gaaccaacgt gatcgagaat cgcaagacgc ccctgatgac
    11341 aacaagccgg ataggcacat gagttttgag atattcctaa aagggtgccg aaaaggctct
    11401 tccaggctac tcaaaaagct cagatcgagg cgccgcgaaa aatcgacgag cgagcgaggc
    11461 gattgagcgg caacgaagcc tatgggcgtt ttcagcaacc tgctaagcgc cggtcctttt
    11521 tcccggggct ttcccttctg cgcttgcatg ttcgagctcg gcgttgattt caccgccgac
    11581 taaaatgacg aatccgctga cgtacatcca ggtgagcagc acgatgatcg cgccgatact
    11641 gccgtaggtc ttgtcgtatg agccgaaatt attcacgtag tacgaaaagc ccagcgaaac
    11701 aagtatccat aagaccacgg caaagacgga ccccggggtc atccatttcc agctctgatc
    11761 gacatccggg gcgaagtaat agatcgcagc cacggccgac ataactaaaa tcaagatgac
    11821 cggccagcgt agaatattcc aggcgatctc gaagactctc cccaaaccaa caaagtcggc
    11881 aatccagccg ccgatctgcg gtccgaacat caagagaacg atcgaggcaa ttagaaagat
    11941 ggataggccg acggttaaga ggattgccat gccgcggact ttccaaaagg gccggccttc
    12001 ttcaacgtca taggcatggt ttaaggcatc cgaaattccg gtaagcgcgc tagatgaggt
    12061 ccaaagggcc gccacgatgc caaacgagag cagcccgcca cgttgttgtg aaaccagttt
    12121 gccaacattc tcttcaacca ggcttaacgc ttcacccggc ataacctcgg ctagcaaacc
    12181 gatgatccgg tccatcagat ttggaatcgg cagataagcc aacaatgcgg tcaaaaaaag
    12241 aaagaacgga aacagcgcaa acaagaaata gtaggccagt gaagccgcat agaccgcgca
    12301 attgtcctcc tgcacttcgg cgataacccg tttgatgagt tgcatcgcac tcaccccacg
    12361 cagcgaaaat gtgtctttga gatcttgctt gaaacccatg agtgtcagcg gccatcgggc
    12421 gacctaagta gaagtccccg atgccgtgca aaaaccgcaa agcacatgcc atgcaaaagg
    12481 aaggaaaatt gttccgagcg cgtccggccg atgtctcacc cgtgcgaaaa gaacccttca
    12541 actctccatc tacgggacga tgataacacc ggtgaagtca ggtgaagaaa catctcacgt
    12601 gatgacttag catcggcccc ggaacctaac tggcgatggt ttcagagcgg aagatttgcg
    12661 aggagcaacc gcagttccgg tggtctactg gattttcaac acttttggtt tatcatatat
    12721 gtctccgaaa aatgtcatgg aatatagaac agacgaccta cgcataaagg aaatcaagga
    12781 gcttctaccg ccggcacaag tgctgcgcga tatcgcgatc acgaccaagg ccgccgaaac
    12841 ggtctatcag acgcgccaag caattcaccg gattttgcac ggcgccgatg accgccttct
    12901 ggtcatcttg ggcccatgtt cgattcacga tgttaaagcc gctaaggagt atgccggcaa
    12961 gctcaaagaa gttaaggatc gtctcagcag tgatctactg gtggtgatgc gtgtgtattt
    13021 cgagaaaccg cgcaccactg tcggctggaa ggggctgatc aacgatccgc aactcgatgg
    13081 cagctttcgg atcaacgaag ggctgcgcat cggccgtcga ctgctgctcg atctcaacga
    13141 aatggcggtt ccggcgggtt ccgagtttct cgacatgatt acgccgcaat atgtcgctga
    13201 cctggtttca tggggcgcca tcggcgcgcg caccaccgaa agtcaggtgc accgggagct
    13261 tgcttcgggc ttatcgtgtc cggtaggttt caaaaacggc actgacggca atgtccgcat
    13321 cgccgtcgac gcgatccggg ccgcccaggc gccacatcac tttctgtccg ttaccaaaga
    13381 cggtcactcg gcaatcgtct ccaccacggg caacgaagat tgccatgtca ttctgcgcgg
    13441 cggccgcaat cccaactacg attcaaaaaa tatcgatgcg gcggccaaaa gtatggcgga
    13501 agccggcata tcggccagga ttatgatcga ttgcagccat ggcaacagcg gcaaagagcc
    13561 ggaaaagcag gtcgaggtcg ggcagcaggt ctcagcccag attgccgatg gcgatgcccg
    13621 gatcttcggc gttatgatcg aaagccacct caaagcaggc cgtcaagatc tgatcccggg
    13681 taaagagctg atttacggtt tgagcattac agatgcctgt gtcgggtggg aagacacccg
    13741 taaagtggta gaccatctcg cacatgcggt tcgccagcgc cgcctcaagg cggcagc
//
LOCUS       DASUVS010000389         7961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_389811, whole genome shotgun sequence.
ACCESSION   DASUVS010000389 DASUVS010000000
VERSION     DASUVS010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7961
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389811"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VF182_20785"
     CDS             <1..222
                     /locus_tag="VF182_20785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439565.1"
                     /translation="RGASDDILEETYEYTKSSLEEVPTPSLEVIKAALEILSHQYPQA
                     KETDANLIIDTSMMRRIEQSGFIKALYKK"
     gene            complement(267..440)
                     /locus_tag="VF182_20790"
     CDS             complement(267..440)
                     /locus_tag="VF182_20790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439566.1"
                     /translation="MMTGKKQTSLSGYHRDGFMSMDLPAMAPTYFHGSSKLFWKSAVR
                     EPSREALGSHATG"
     gene            444..1448
                     /locus_tag="VF182_20795"
     CDS             444..1448
                     /locus_tag="VF182_20795"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439567.1"
                     /translation="MFRVINFIAEFLIVASLFTGGQVFGATKLEPLTVGYSNITATYA
                     PLWIAVEERVGMKYGLDLKAIYAGRVRPQQLLATGDVPVVIASGTGTMTSHIVGVKDQ
                     VLVATITSKIGTSLFAKPEIKSVEELKGKTVATGRPAAFLDATVRYVLRSKFGLVPDR
                     DVKLLPTGEPYLGLQALERGIVDAAAMSIPHLFIARKAGFKELVSFDKLGVEYPYTSV
                     VVLRQTAAKSPDLVERLVKCIVEGIHIFKTDKAKALAALRLYMKGADEGILEESYQHT
                     RGTIEDAPYPSLQVVKAGLEMLSLQYPQAKQTDASLIIEPAFMKRIADSGFVRALDKK
                     "
     gene            1562..2602
                     /locus_tag="VF182_20800"
     CDS             1562..2602
                     /locus_tag="VF182_20800"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439568.1"
                     /translation="MLDGKLRIAPTCYHMLHQVPVMTAHEMNFFYDEGLVTPHGTLGY
                     EILTESMVPFGLEKLGISQAMKEKSIDIALDVQSRTVFYQRARGADLYILCGWRNQHT
                     NVWMAPPHIKSLQELKGKRVGISDFNSIRHWAIQIQLKKAGLDLERDIEWVRLGVNAR
                     FHVDAIRNGKVECAPVPPWSAEDLKKEGCNALVSPADQYPNGRPERIIAVTGRILEER
                     PDLVKSFLRGIIRSYWFIRDMPKNYEYVNNLEKRLRLKSADPEERIVTHLRTPRDLEA
                     MPFPLNGRATGFEDMLKEEERLGELNYQVPPIQEVAAQDLVDEAYKELRGRQELGQEY
                     ERVSKVEQRWGY"
     gene            2712..3761
                     /locus_tag="VF182_20805"
     CDS             2712..3761
                     /locus_tag="VF182_20805"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6439569.1"
                     /translation="MNLVKSLYWARIIPGATLVSLVLALPLHAQTSKNFYEGKIITFL
                     VGSSAGGGTDLTARVIARHIERYIPGKPRIDVANKPGAGGMISANELYNLKKPDGLSI
                     STLNTGSLFATATGNQAIKFDLQKFIWLGQAFDDAQVLYLKTSTPYTSFEAVRKANSA
                     GKQPKMGAQALDHTSNVVVKIAEQIFGLDFQVIPGYPGTPQILLDIERGALDGRAQGI
                     GSLMATRREWIKSGYIKILATSKKRRDPRIPDVATMEELAPPGSKGLLQALYAGQNLA
                     RSVVLPPGVPADRVKILRDAFAAMTRDELFLKESEKLGLEVDLIRGEDMNRDIEGTLR
                     DKRLMDLYRMIASAK"
     gene            3859..5307
                     /gene="glmL"
                     /locus_tag="VF182_20810"
     CDS             3859..5307
                     /gene="glmL"
                     /locus_tag="VF182_20810"
                     /inference="COORDINATES: protein motif:HMM:NF040745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HEX6439570.1"
                     /translation="MSSLALLIDFGSTFTKVIAVDLDASRVVGRSQAPSTVLTDVREG
                     LLQALSALHEHHSLFDQRPSSLDILSGHLALASSSAAGGLRMAVIGLVPGLTLLAASQ
                     AALGAGAKVVGSFAFKLANNSIEEIVRLRADMVLLTGGTDGGDSATILHNARLLANST
                     ISVPIIVAGNQAVATQVGEILEAGGKEVRPTDNVMPRSGVVAVDAARDEIRKLFIERI
                     IHARGMAAIGEFVSVVLPTPMAVLKGVRLGAVGTDREEGWGEMVVIDVGGATTDVHSI
                     GYGYPVGENVIKQGLPEPYAKRTVEGDLGIRFNAATLLAQVGPDGFDAQWRAMFPQLQ
                     VSRDEIGRYITEITEKTARVPEQKWHTLADAVLARIAVDLALERHAGKRERVVTSTGE
                     AWVHYGKDLRNTPRLIATGGVFVHNPYGSMIVSSSGQEQVDSTVLRPKHPKIYLDLSY
                     ILYAVGLLENDYPDLAVRLFKKYMSPIADSED"
     gene            complement(5394..6953)
                     /locus_tag="VF182_20815"
     CDS             complement(5394..6953)
                     /locus_tag="VF182_20815"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6439571.1"
                     /translation="MYNDYFGFLAPPFSATPDSRIFYDNELYREAFANLRYGIEWRKG
                     LVVLTGEVGTGKTTLLDKIIRDLGSIIQVVFISYDQLNSTELIQLVSRELGLDSDRHD
                     RVLKIEQIREYLITEFRRQRIVALLIDEAQNLSQETFESVRFLSNFQYGGEKLLQIVL
                     TGQPELQMRLNQPKLHHLKQRIVIHSRLAPLTNDEVGRYILFRVRSAGFRGKELFPTE
                     VLGDISLFSGGIPRLINIICDNALLLAYAGGKKIITAEIVQEVARDLGLGNLSQAQIA
                     AASAHREIRDAGLQTTPAISLIPAAVPQQYRRNRSLAWVPVGMVSALIALGGAGGALY
                     SQQIKGYLGQESSSRNHQPAPLPIRQNRPVMFRTSEPEISLELHQSVTPVQAKPEDLP
                     KDPVIAQAPAPSSEQTQPQESSPKKNDSRLGVFQVVGQSSFVRGSPRADAKILATLEP
                     STRIKVLSAKGDYLQVQTVFEGKAIRGYVHREDAFFERPTKDKRPPSASSLVSGGQLN
                     NPSAQQSRDKP"
     gene            complement(7516..>7961)
                     /locus_tag="VF182_20820"
     CDS             complement(7516..>7961)
                     /locus_tag="VF182_20820"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX6439572.1"
                     /translation="STVFQIANDAYGSRSAILGMDLIAEFNPSISNLNWISAGEDLLL
                     PALNEDTLLRQQPDGSFRLIVASFLSRREAEEFAQQIARDGLSAFVTAKAVSNNLVLH
                     RLEITGLKNLEHARQTIEIGFKSRWAPFAPKPLIETEASQAVTGY"
BASE COUNT         1848 a         2032 c         2269 g         1812 t
ORIGIN      
        1 cgcggagcaa gcgacgacat cctggaggag acctatgaat ataccaaatc cagcttggaa
       61 gaggtgccca cgccatcgtt ggaagtgatc aaggcagcgc tggaaattct gtcgcaccag
      121 tatccgcaag ccaaggagac cgatgccaat ttgattatcg atacatcgat gatgcggcgc
      181 atcgagcaga gcggtttcat caaggcgctg tacaagaagt gacgcgcgac ggagttgcga
      241 gagtttcact cgagtaaacc aactcatcat cccgtcgcgt gcgaacccaa cgcctcgcgc
      301 gagggttcgc gcaccgcgct cttccagaat aatttagaag agccgtgaaa atacgtcggc
      361 gccatcgcgg gtaaatccat tgacatgaaa ccatcacgat gataaccgga aaggctagtc
      421 tgtttttttc cagtcatcat cctatgttcc gagtcatcaa ctttatcgcc gaatttttga
      481 tcgtcgcaag cctgtttacc gggggtcaag tgttcggcgc cacaaagcta gaaccgctga
      541 ccgtcggtta ttccaatatc accgcgacct atgctccgct ctggatcgcc gtcgaagagc
      601 gtgtcgggat gaaatatggg ttggacttga aagcgattta cgccggtcgg gtgcgtccgc
      661 agcaattgct tgccaccgga gatgtgccgg tggtcattgc ctccggcacc ggcaccatga
      721 cctcgcatat cgtcggggtc aaggaccagg tgctggtggc gaccattacc agtaagatag
      781 gcacatcgct ctttgccaag ccggagatca agagtgtcga agagttgaaa ggtaagaccg
      841 tcgcgaccgg ccggccggcg gcttttttgg acgcaaccgt gagatatgtg ttgcgcagca
      901 agttcggact tgttccggac cgggacgtga agttactacc caccggggaa ccttatctcg
      961 gtctgcaagc gctggaacgc ggtattgtcg atgccgccgc aatgtcgata ccgcacttgt
     1021 tcatcgcgcg aaaagccggc ttcaaggagc tggtaagttt cgacaagctc ggcgtcgaat
     1081 atccctatac cagcgtggtg gtgttgcggc aaacagcagc caagagtccg gatctggttg
     1141 aaaggttggt caaatgtatc gttgaaggaa tccatatctt caagaccgac aaagcaaagg
     1201 cattggcggc cttgagactc tatatgaaag gtgccgacga aggaattcta gaagaatctt
     1261 accagcacac tcgcggtacg attgaggacg cgccttaccc ttcgctgcaa gttgtcaagg
     1321 ccggattgga gatgttatcc ctgcaatatc cgcaggcgaa acaaaccgat gcgagtctga
     1381 ttatcgagcc ggcgttcatg aagcgaattg ccgacagcgg cttcgttcga gcgctggata
     1441 aaaaataact tcgttcgaga cgttccaagt ccttcattcg cccacaggat gggcgattta
     1501 agcgcgggga gaatcattaa acctaacgtt cgcatccatt cagaaatggg gaactgctgc
     1561 catgctcgat ggtaaattga gaattgcgcc cacttgttat catatgttgc atcaagtgcc
     1621 ggtgatgacg gcgcatgaga tgaatttctt ttatgacgag ggcctggtta cgccgcatgg
     1681 aaccctgggg tacgagattt taaccgaatc gatggtgccc ttcggacttg aaaagctcgg
     1741 catctcgcaa gcgatgaaag agaaatccat cgatattgcg ctggacgttc agtcacgcac
     1801 ggttttctat caacgggcgc gcggtgccga cctgtacatc ctgtgcggct ggcgcaacca
     1861 gcataccaac gtatggatgg cgccgccgca catcaagtcg ctgcaagagt tgaagggcaa
     1921 gcgggtcggt atcagcgatt tcaatagcat tcgccattgg gcaattcaga ttcagttgaa
     1981 gaaggccggc ttggatttag agcgcgatat cgagtgggtg agactcggcg tcaatgcgcg
     2041 atttcacgtc gacgcgatcc gtaatggcaa agtcgaatgc gcccctgtgc caccttggag
     2101 cgccgaagat ctcaagaaag aaggctgcaa tgcgttggtt tcgccggcgg atcaatatcc
     2161 caacgggcgc cccgaacgta tcattgcggt taccgggcga atccttgagg agcggcccga
     2221 cttggtcaag agttttctca ggggaattat ccgcagctac tggtttatcc gcgacatgcc
     2281 gaagaattat gaatacgtca acaatctgga aaagcgcttg cggctcaaaa gcgcggatcc
     2341 ggaagagcgg atcgtaacgc acctgcgaac cccgcgcgat ctggaagcca tgccgtttcc
     2401 gttgaatggc cgggccacag gcttcgaaga catgctcaaa gaagaagagc ggttgggcga
     2461 gctgaattat caggttccgc cgatccaaga ggtggcggcg caggacttgg tggatgaagc
     2521 ctataaagaa ttgcgcgggc gacaagagct tgggcaagag tacgaacggg tcagcaaagt
     2581 cgaacagcgg tggggctatt aggacgattt gcgcttcgcg ctgttccgat accccaaacg
     2641 cgcagatcgt tcagaggagt ttgaatgagc gccgctggga atttactcag tcgtatttaa
     2701 ggagttaatc agtgaaccta gtaaagtcgt tgtattgggc aagaatcatt cctggtgcga
     2761 cgttggtctc gctcgtgctg gctttgcctc tccacgcgca gacctccaag aatttctatg
     2821 agggcaagat cattacattt ctggtcggct cttcggccgg cggtgggacc gatcttaccg
     2881 cgcgtgtgat cgctcggcat attgaacgtt acattcctgg caagccgcgt atcgacgtgg
     2941 ccaacaagcc gggcgcaggc ggcatgattt ccgccaacga gctctacaac ttgaaaaagc
     3001 ccgacggcct gagcatcagc acgctcaata ccggaagcct gttcgcgact gccaccggca
     3061 atcaagcgat caagttcgat ttgcaaaaat tcatctggct gggccaggca ttcgatgatg
     3121 cgcaggtgct ttatctcaaa acttcgacgc cgtataccag tttcgaagcg gttcggaagg
     3181 cgaatagcgc agggaaacag cccaagatgg gcgcccaggc gctcgatcac accagcaatg
     3241 tcgtcgtcaa aatcgccgaa cagattttcg gtctggattt ccaggtgatt ccgggatatc
     3301 cgggcacgcc gcagatcctc ctcgatatcg agcgcggcgc gctggatggc cgcgcgcaag
     3361 gcatcggttc tttgatggcg acgcgacggg agtggattaa aagcggttac atcaagattt
     3421 tggcgacatc gaagaaacgg cgcgatccgc gcattccgga tgtagcaacc atggaagagc
     3481 tggcgccgcc gggcagcaaa ggcttgctgc aagcattgta cgccgggcag aatctcgcgc
     3541 gctcggtggt cttgccgccc ggagtgcccg ccgatcgcgt gaaaatccta cgcgatgctt
     3601 tcgccgcgat gacccgagac gagcttttct taaaggaatc tgaaaagttg ggcctggaag
     3661 tggatttgat acgcggcgag gacatgaacc gcgacatcga aggcacgctg cgcgataaac
     3721 ggctgatgga tctctatcgg atgatcgcat ccgccaagta aatcgctggt agcttcgaag
     3781 tccgggtgga gcaggcattc tccgtctgat gccggctgta aagctccctt tcgttgtttc
     3841 aagtgtccat gcttttaaat gtcatccctt gctctcctca tcgacttcgg cagtaccttc
     3901 accaaagtca tcgccgttga ccttgatgca tctcgagtgg tgggcaggag ccaggctccg
     3961 agcacggtgc taaccgacgt gcgtgaagga ttgctccagg cgctatccgc actgcacgaa
     4021 caccattcac tgtttgacca gcgaccgtcg agcctagaca tcttgagcgg tcacctggcg
     4081 cttgcttcga gcagcgccgc gggcggtttg cgtatggcgg tgatcggcct ggtccctggt
     4141 ttgacactcc ttgcggcaag ccaggcagct ctgggagccg gcgcgaaagt tgtcggctca
     4201 tttgcgttta aactggccaa caactccatc gaagaaatcg tccggttgcg tgcggatatg
     4261 gtgcttctta ctggcggtac tgatggcgga gatagtgcaa cgattctgca caacgcgcgc
     4321 ctgcttgcga attctacgat ttccgtgccg atcatcgttg ccggaaacca ggcagtcgcg
     4381 acgcaagttg gcgagattct agaagccggc ggtaaagaag ttcgtccgac tgacaatgtc
     4441 atgccgcgct caggcgtcgt cgcagtagat gccgcgcgcg atgagatacg caaactgttc
     4501 atcgagcgaa taatccatgc gcgaggaatg gcggcgattg gtgaatttgt ttcggtggtg
     4561 ctgccgaccc cgatggccgt tctcaaagga gtgaggcttg gagccgtcgg caccgatcgg
     4621 gaagagggtt ggggcgagat ggtcgtgatc gacgttggtg gcgcaaccac cgatgtgcac
     4681 tcgatcggct acggctatcc ggtcggggaa aatgttataa agcaaggcct cccggagcct
     4741 tacgccaaga gaaccgtgga aggagatttg gggattcgtt ttaatgccgc cactcttctg
     4801 gcacaggtcg gtccggatgg ttttgatgcg cagtggcgcg cgatgtttcc tcagttgcag
     4861 gtctcgcgag atgagattgg ccgctacatc accgaaataa ccgaaaaaac cgctcgtgtt
     4921 cccgaacaaa aatggcatac gctagcggat gccgtgttgg cgcgcatcgc cgtagatctt
     4981 gctttagagc gtcatgcggg caaaagagaa agggttgtga cttcgacggg tgaggcgtgg
     5041 gtccactatg gtaaagattt gcgcaatacg ccgcgcctca tcgcaaccgg cggcgtattc
     5101 gtgcacaatc cttatggttc catgatcgta tccagttccg gccaagagca ggtagactcg
     5161 acggtcttgc ggccaaagca cccgaaaatc tatctggatt tatcctatat tctatatgcg
     5221 gtcggattgc ttgaaaacga ttacccggat ctggcggtgc ggctgtttaa gaaatatatg
     5281 agccccatcg ccgattctga agattagctt cagccggtgc aaacgcgcat agggctctgg
     5341 acagattctt atccgcatat cgatcgactc tgacgggatg acagcacgtg tctttatggt
     5401 ttgtctctgg actgctgcgc gctcgggttg tttaattgac ccccggacac gagactgctc
     5461 gcactgggtg gccgcttgtc tttggttgga cgctcaaaaa aagcatcttc gcgatgaaca
     5521 taaccgcgaa ttgccttacc ttcaaaaacc gtttgtacct gaagatagtc tcctttcgca
     5581 ctgagcacct ttattcgtgt ggacggttcg agcgtagcta gaattttggc gtcggcgcga
     5641 ggactgccgc ggacaaacga tgactgtccg acaacttgga agactcctaa acgcgagtca
     5701 ttcttcttag gagagctttc ttgaggctgg gtttgttcgg aactgggcgc gggcgcttgc
     5761 gcgataacag gatctttcgg gagatcctcc ggcttcgctt gcactggagt tacactctgg
     5821 tgcagttcta aagaaatctc tggctcgctc gttcggaaca tcaccggacg attttgacgg
     5881 ataggaagcg gtgccggctg gtgattccgc gatgaggact cttgcccgag gtatccttta
     5941 atctgctggg agtacagcgc gccaccggcg ccgccgaggg caattaacgc cgagaccatg
     6001 cccacaggta cccaagctag acttcgattg cggcggtatt gctgcggaac agcggccgga
     6061 atgagcgata tagccggggt ggtttgaagg ccggcgtcac gtatctcccg atgcgccgaa
     6121 gcggcggcaa tttgtgcctg ggataggtta cccagtccaa gatcgcgcgc gacctcttga
     6181 acgatttcgg ccgtaattat tttcttgcca ccggcatagg cgagaagtag tgcattgtcg
     6241 cagatgatgt taatcaagcg gggaataccg ccggaaaaca aactgatatc cccaagtacc
     6301 tccgtcggga acaattcttt accgcgaaat cccgcactac gaactctgaa aagaatgtag
     6361 cgaccgacct catcattggt cagaggagca aggcggctgt gaatcacgat ccgctgcttg
     6421 agatggtgga gttttggctg gttcaggcgc atttgcagtt cgggttggcc ggtcaataca
     6481 atctgtagta atttttcgcc gccatattga aaatttgaaa gaaagcgaac cgattcaaat
     6541 gtctcctggc tcaagttctg agcttcgtcg ataaggagtg cgacgatgcg ttgcctgcga
     6601 aattctgtaa tcaaatattc tcgaatttgc tcgattttca aaacccggtc gtggcggtcg
     6661 ctatccaggc ccagctcccg ggaaaccagc tggatcagct cggttgagtt gagttgatcg
     6721 taagaaatga agacaacctg tataatcgag cccaaatctc gaatgatctt atcgagcagg
     6781 gtggtctttc ctgtaccgac ctcgccggtg agaactacta aacctttcct ccactcaatg
     6841 ccgtaacgca gattggcgaa ggcctcgcga tagagttcgt tatcataaaa gattcgagaa
     6901 tccggcgtcg cgctgaaagg cggcgctagg aagccaaaat agtcgttata cataacaagg
     6961 ccgatgccgg tctttattaa acagcttcaa cttcgcgttc agaaccctag tgacgctaga
     7021 cggaagtgcc gataacatgg agagcgatcc gttgcgccgg atcgagactg cctattttct
     7081 tgatgtacct gaacctcagt gactcggctt atcattttat aatcttcgaa caatcgtttc
     7141 ttaataaaac aggggatatt gttcgcatac tgttgcaatg gttgtcaaga atgtttttag
     7201 attcttcgcg gggggaattt aagaccgttg cggcgtgcga atgtctcacg cgcgatatca
     7261 gaagctcgac cggaatagca ggcggttcat gagtctcagt gcgagtccac cacatgcttc
     7321 ggtgcctgtc aggaaatgcc atttccccgt atcgaagatc aatgccggtg ccagcgcacc
     7381 acgcgccaga acacaatcag gcggatcgtt cgaaaagcgt cttcggtgcc gctttggaat
     7441 tcaagaaact cggcgcgatc aagagagctg cttaaattaa ggatgcaacg ggtattgctg
     7501 gtgcgcagcc ccgagctagt accccgttac tgcctggctt gcttcagttt cgattagcgg
     7561 cttcggagcg aatggggccc aacggctttt gaagccgatc tcgattgttt gccttgcgtg
     7621 ttcgaggttt ttcaacccgg tgatctcgag ccggtgcaac acaagattat tcgaaactgc
     7681 tttagcagtg acgaacgctg aaaggccgtc acgcgcaatt tgctgggcga actcctccgc
     7741 ttctctgcgg gagagaaacg atgcgacgat aagacggaaa gaaccgtccg gctgctggcg
     7801 cagcagggta tcttcgttaa gggcaggcag taacaggtcc tctcccgcgc taatccagtt
     7861 taaatttgaa atgcttgggt tgaactctgc aatgagatcc atacccagaa tcgcgcttct
     7921 gctgccatag gcgtcattag cgatctgaaa gactgtcgag c
//
LOCUS       DASUVS010000390        12446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_826170, whole genome shotgun sequence.
ACCESSION   DASUVS010000390 DASUVS010000000
VERSION     DASUVS010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12446
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826170"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            500..2152
                     /locus_tag="VF182_20825"
     CDS             500..2152
                     /locus_tag="VF182_20825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439573.1"
                     /translation="MNNRRFRLLSRSCSSITALLTLIMFLTSSMRPAWAMDSARGGAL
                     PQPLPLFPADNWWNLDISSWPVDPNSASFISFINNGATRRLHPDFGGDAGTGDAIYGM
                     PYAVVANVADADLKAVQFQYSDESDGVEHSTDISFPFYPIPPDAITQPFWIEGGDPGN
                     VDLRSSQDRHLLIVDKERNHLYELYNVFYNATQNKWFAGSGAFFDMNTNNRRPDTWTS
                     ADAAGLAVLPGLIRYDEVYDPTLAEIRHAFRMTVRATNGYVYPASHRAGSTAGALPLG
                     ARLRLKASVDVTQRTSDPNIQKIFRAMQTYGLIVADNGSDMYVTGTYDTRWNNDILNP
                     AFRNLTASDFEVIELGYNPPAAAPASLASVSIKPSSITGGQLATGTVTLSGAAPAGGA
                     IVSLASANPAASLPNSITVPASASSANFPVSTLTVTATTAGNITASYAGISKSVTLTV
                     NPAVPVALLSLSVNPKTVVGGSSSVGTVTLSKVTSSPVIVSLASSKPKNAMIPASVTV
                     PAGASSAAFNISTTTTNKKINATISASYGGATKSATMTIGRR"
     gene            2552..3547
                     /locus_tag="VF182_20830"
     CDS             2552..3547
                     /locus_tag="VF182_20830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX6439574.1"
                     /translation="MTNFNARRCLLLVVLIAITAIAGFAGLANAQVKTRLSIATGGTG
                     GVYYPLGGGLAALISKHIPGVEATAEVTTASVDNMKLLHANRVALAFTLPDSALDAYQ
                     GQSKGLKEKVAVRSIAAIYSNYMHIVALDGIGIKTVADLKGKRVSTGAPGSGTEVKGL
                     RVLEAYGISPKDLGSQDRLGAAESAGALKDRKIDAFIWDGGLPTAAVLDLGATPGIKI
                     RLIPHGDAVPKMVAKYGPLYFVANIPEGTYGGMDADVPVAAVTNLLAAHERLEEPLAY
                     QITKLLHEHTPELVAVHSAAKEITLKNAVTGSPIPFHPGALRYYKEKGIKVSGAQ"
     gene            3568..4020
                     /locus_tag="VF182_20835"
     CDS             3568..4020
                     /locus_tag="VF182_20835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439575.1"
                     /translation="MTGFFFLPFACLLVTAAAAQPICLNVQTNSGSAHYAITAGDELY
                     LTFPHSIYGSQVEEHFRATSEGFQLTELRYAEPRLIEFYGYESAVKKNGWWIVSKRGP
                     LMAILDLRVSPGSWIGVIFGTEKLTVNHDSADGRARLALSACRRSNHG"
     gene            4013..5959
                     /locus_tag="VF182_20840"
     CDS             4013..5959
                     /locus_tag="VF182_20840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEX6439576.1"
                     /translation="MADEVIDSSRIEAAKEIAQEEEGPRRRLAGPLSWLAYSLAAAMS
                     LVFIYWAWTTVTTQILRLVFLAFTLVLTFLLYPPTKKARLRSVIWFDWILVALSLLAL
                     GYPLWDFEEFIYRAALPDLKDQIFGALTIVLVLEATRRTSGWILPVVALVFLAYAWLG
                     AWLPSPWTHQGYGLDRLVGHMYMTLEGIFGVPLDVAATFIILFTIYGAVLEHSKAGEF
                     FVNFSMAATGGKRTAAGRTVTLASFLLGGPSGSGVATTITLGSVAYPLLAKAGYDKDS
                     AGGLLSAGGIGAIISPPVLGAAAFLIAEFLRISYLEVIALAAIPTLLFYWSIFVMVEL
                     DAHKFGARPVTVETEGVWSLTRKYGYHFISLVAIVIFMVWGYTAISSVVYAILVSVVL
                     SFLRPETALTPPRLWRALIDGSTGVLSVAATCATAGIIVGVVTLTGLGLKFSMIILTM
                     AGGNLFLTILYTGLAVWILGLAMPVTASYIIAAVITAPAMIKLGVPDYAAHMFIFYYA
                     VLSEVSPPTALSPFAAAAITGGNPYKTTMMAWKYTLPTFLVPFMFTLHPDGAGLLLKG
                     PWENVVWTSVTAALGLAALAAGLTGWMLMKTTWLERGLLLAAGLVLVYPSLIQDVIGL
                     VLFASAGSLQYFRRRSGGETLPAH"
     gene            complement(6544..8580)
                     /locus_tag="VF182_20845"
     CDS             complement(6544..8580)
                     /locus_tag="VF182_20845"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX6439577.1"
                     /translation="MNFRLSFRSIALLSIGSMLFPLGRGNVRAAGAPSSRFHLEEATI
                     ADVHRAIRAKQITATQLVQFYFKRIEAYNGICVKGDVDPATGLMLGDLTPIENAGQLN
                     AYMTLNIRGKRSKTDSADNDPKLPDALEVAKAQDAHFARTGRLVGALHGIPFAIKDNY
                     DTVDMRTTAGAAADYANDKPPQDATMIAKLRAAGAIFLGKTNLDEYAPASIARSALGG
                     QTCNPYDTKRVPGGSSAGSGAAVAANLAMCALGTDTSGSVRFPSTANALVGMVATQGL
                     VSRAGIIPLSFSRDRGGPMCRTVQDTAAVLEVVAGADPRDHVTAPAHGKKPVPYRNYA
                     GRKSLAGKRLGVVREFMIEATIADRDSIRIANQAIADMKRLGATIVDPVNFDGAIAEI
                     MASYEPSFFTQTFKSAVASGSDPIDRMVEISDDPKTLPTGMRGVNLRMIAGQRRGEEG
                     KYAMNLYLRQRGDAKFRSVEDMFATKTFSGDLPVLQNAFGAKAKTLETPAHTSHILRM
                     QNLQRILYKVMADNNLDALVYPYSTIPPHTVLANRTPEVYDTHTEPRVLKAGTELSDP
                     NLLPGETKLKNDLDLWRGAGSSWSVNLSPVSGFPAIVVPAGFTREVYDRIPDANDPNR
                     SRLDGPKPDQIPVGMEFLGRPFDEATLFEITSAYEAGTKHRRSPKAFGPLKEEP"
     gene            complement(8832..10019)
                     /locus_tag="VF182_20850"
     CDS             complement(8832..10019)
                     /locus_tag="VF182_20850"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEX6439578.1"
                     /translation="MTIPFRPRWYFFVALLSVTFIGPLSLHLFIPAMSAVKDAFDVST
                     GVAQLTMSLAMLSMAFFTVAYGGLSDRLGRKRVLLAGLVLFTCGAAACMVAPNMPMLL
                     AGRILQGAGAGCGVVLARAIARDVYGQERVAQVIAYLTAAYVLGPMFAPPVGGQLTAA
                     FGWRALFVLASVVGLLVIFAVIFGVPETRVQSAAAPRSVFAGYKSLLRRLRFVGFMLQ
                     PGLMSAAFYTQATAASFLAAEHLGADPAKIGWWFFAFPIGFMAGSFISGRIGAKRSIE
                     FMTILGGVIGVVNGALLVAWFYFGGISMAALYIPGTFVSLAQGLSMPYAQAGAMAVDP
                     ELSGSASGAVVFSQFFWPAALQQLTGLLADGTWVPMAAVHGAAVIGALVAAWMAARAK
                     GQN"
     gene            10501..11487
                     /locus_tag="VF182_20855"
     CDS             10501..11487
                     /locus_tag="VF182_20855"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439579.1"
                     /translation="MMKKLSILAGVLLFAFSSAVGAQEKIRIGIGSVSLQSVLPFIGK
                     QRGIFARYNLVPEIIYIPGGSTNVQALISGNLDLSQLSGAPGAAANLEGADIIYILGL
                     LDKLNYQLITRPEIKNIEQLKGKRFGISRFSSSADFGLRALLKKVGVDPIKDVTILQI
                     GDEHARLAAVKAGNIDGTVMNAPFGAEAEKLKLNVVADSVKMGIPFFNTGLLGSKKFF
                     DQNEAKVLNFLRAYLESIKVFKTEREYAVKSTAQFTRVNNIKAIEEGYDYFKEQLRNV
                     PYPSAEAMQAVVAQLAETNVKARKIDGKAYVNDRYLRQLEDEGFVKKLWESK"
     gene            11539..>12446
                     /locus_tag="VF182_20860"
     CDS             11539..>12446
                     /locus_tag="VF182_20860"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6439580.1"
                     /translation="MLAMVSFVFIFFLSHAAPGAEADAQRQLVEGAKKEGKLVFYTSV
                     ETEFARLLTTGFEAKYPFVKTDIFRSNHERIFSRLNVERKAGTFAGDVLSVGEFETYH
                     MQKRGLLTPYKAPSAAVYPEGFKDPDGYWTDFYDNLIVSAYNTTRVKREELPRNYEDL
                     LHPRWRRRMVLDRNEDRWFANMLHHMGEKRGMEFMQALAKQEVAIRSGRSLITQLMAA
                     GEFDLQIVAYWYRPYLMNKQGAPVDWFSLEPAFVALHPISIIERAPHLNTAKLFIEYV
                     LSEEGQRLLAKRGREPARPGIRPEGYP"
BASE COUNT         2662 a         3463 c         3571 g         2750 t
ORIGIN      
        1 attcactgac gattggggta gcggttgaac aaccgttgag agcgagcgtg atgaaaagaa
       61 tttccacaat gcctaagggc aacgtctgct tcatggccca tcatggcacc aaatcaacgg
      121 ttgtttaaga acctccctaa aagttccggt tgcggaagtt cagccaagca ccactgcccg
      181 tgggtaaacg attcactccg tgtgagagga tggtgagaga atcggtctcg gcgtttagct
      241 cgctcgggaa gcgctgcgcc gctcctatgc ggcgccggag aagctggcga tttgccggaa
      301 atctcttacc ttcgatcatc ttcttatcct cacaggctgc catacaggac gacattgacg
      361 gtccgtgatt agcggaggct cgtcaccgtt cgtagtctct aagtaactcc taagcatttt
      421 gcgagaacga gtgtcagttc aaaagtggca tatcggttgc gccgatctac cgagattctt
      481 tcaattcgcg gaggtcggca tgaataaccg tcggtttcgt cttttgtcgc gcagctgctc
      541 gtccattact gcgctgctca cattaataat gttcctgacg agctcgatgc gtccggcatg
      601 ggcgatggac tcagctcgcg gcggcgctct gccgcaaccg ctgccgctat ttcccgcaga
      661 taactggtgg aacttagaca tcagcagctg gccggtggat ccgaattcgg cgagctttat
      721 ttcctttatc aataatggtg cgacgaggcg attacatccg gacttcggcg gcgacgccgg
      781 gaccggagac gccatttacg gcatgcctta cgcggtcgtt gccaatgttg ccgatgctga
      841 tctcaaggcg gtacagtttc aatattcaga tgagagcgac ggcgtcgaac attcgaccga
      901 tatcagcttc cccttttacc cgattccgcc cgatgcgatt acccagccgt tctggatcga
      961 gggcggcgat cccggcaatg tcgatttacg cagcagccaa gaccgtcacc tgctgatcgt
     1021 cgacaaagaa cgcaatcacc tctatgagct gtacaacgtc ttctacaatg cgacgcagaa
     1081 caagtggttt gccggctccg gcgccttttt cgatatgaac acaaacaatc ggcgtccgga
     1141 cacgtggacc tcggcggacg ccgctggatt ggcggtactt cccggtctga ttcgctacga
     1201 tgaagtgtac gacccgactc tcgcggaaat tcggcacgct ttccgaatga cggtgcgagc
     1261 taccaacggt tacgtttatc cggcatcgca tcgcgccggt tctacagcgg gcgctttgcc
     1321 gctgggcgcg cgcttgcgct tgaaggcgag cgtggatgtg acgcaaagaa ccagcgatcc
     1381 caatatacaa aaaatctttc gagcgatgca gacatatggt ctcatcgtcg cagacaatgg
     1441 gtcggacatg tatgtcaccg ggacgtacga cacccgctgg aacaatgata tcctgaatcc
     1501 tgccttcaga aatctcacgg ccagcgattt cgaggtcatc gagcttggct ataatccgcc
     1561 ggccgcggcg cccgcgtcct tggcatcggt cagtatcaaa ccgtcgagca ttaccggagg
     1621 ccagttggca accggcacgg tgactctgtc gggcgcagct ccggcgggcg gcgccatcgt
     1681 aagtcttgcg agcgcgaacc cagccgcgtc gctgccgaac tctattaccg taccggcaag
     1741 cgcctcgtct gcgaatttcc ccgtaagcac attgacggtg acggcgacaa ccgcgggcaa
     1801 cattaccgca agctacgccg gcatcagtaa gtcggtgaca ttgacggtca atccggcggt
     1861 cccagtagca ctgctatctt tgagcgtgaa tccgaaaacg gttgtgggcg gctcgagctc
     1921 ggtgggaacc gtcacgctga gcaaggtgac ctcgagtcct gtgatcgttt cgctcgccag
     1981 cagcaagcca aagaacgcga tgattccagc aagcgtcacg gtaccggcgg gcgcgagttc
     2041 agcggccttc aacatcagca cgaccaccac taataaaaag atcaatgcga ccatcagcgc
     2101 gtcttacgga ggcgcgacga agagcgcgac gatgacgatc ggcagaagat agagtttatc
     2161 agcttccggg aagaggccat ctttggataa gaccgaaggt ggcctctttt attccagaac
     2221 tctgtctttt tggctcgctc ttctatcgag gttttcgaga ataatccgga aaaagcatgc
     2281 cggtcaggat aaggctgtta gagaagtcgc gtatacagtc acgagccgcc aagcagcttg
     2341 acctcgctac gataactcgt tgatatcccg ccccgtcgtg tgaacgagtg ggaacgcgcg
     2401 ctccacacat taactcctca ccgaaaccgg cgcttcggaa aacgccttga agtcgactcc
     2461 agcaatggtt ttactcctac tatgtgatct cgatcccaaa gttgcctgga aactttcggg
     2521 tcttggaaaa aattcacagc caggaggtac tatgacgaat ttcaacgcgc gacgctgttt
     2581 gctgctcgta gtgttaattg ccattactgc aatcgctggt ttcgcgggac tggcaaatgc
     2641 tcaggtaaaa acccgtctct cgatcgctac cgggggaacc ggcggtgtct attatccctt
     2701 gggtggcggc cttgcggcgc tcatctccaa gcatatccca ggggtggagg cgacggcgga
     2761 agtgaccacc gcctccgtgg acaatatgaa gcttctgcat gccaataggg tggctctcgc
     2821 gtttacattg cccgatagcg ctctggacgc ctatcaagga caatcgaagg ggctgaaaga
     2881 aaaagtcgcg gttcgttcca tcgcggccat ctactccaat tacatgcaca ttgttgcgct
     2941 tgatgggatt gggatcaaaa ccgtggcgga tctcaaaggc aagcgagtct ccacaggcgc
     3001 tccgggttcc ggaacggaag taaaaggcct gcgggttctc gaggcttatg gaatcagccc
     3061 caaagatcta ggaagtcagg accgtttggg cgccgccgag tccgccggcg cattgaagga
     3121 tcgaaaaatc gatgcgttca tctgggatgg cggtctgcca accgcagcgg ttcttgattt
     3181 gggcgccaca ccgggaatta agattcgcct gattccgcac ggcgacgcgg tccctaagat
     3241 ggtcgccaag tatgggccgc tatattttgt cgccaatatt cctgaaggca cctacggggg
     3301 catggatgcc gacgttccgg tcgcggcggt gacgaatttg ctcgcagcgc acgaacgttt
     3361 ggaggagccg ttggcctatc agatcaccaa gttgttgcat gagcataccc cggagctcgt
     3421 ggcggttcac tcggccgcca aggaaatcac gctgaagaac gcagtcaccg ggtcgccgat
     3481 cccgttccac cccggcgcgc tgcgatacta caaggaaaag ggcattaaag tttccggtgc
     3541 ccagtgatct cttctcgctt gcggcgaatg acaggatttt tcttcctgcc gttcgcctgc
     3601 cttctggtca cggcagccgc agcccaaccc atctgcctga acgtgcagac gaatagcgga
     3661 agcgcgcatt atgccatcac cgccggtgac gaactttatc tgacttttcc gcattccatt
     3721 tatggctccc aagtcgaaga acatttccgc gccacttctg aaggatttca gctgactgag
     3781 cttcgctatg ccgagccacg cctgatcgaa ttttatggct atgagtcggc cgttaagaaa
     3841 aacggctggt ggatcgtctc gaagcgcggg cctctgatgg cgatactaga ccttcgtgta
     3901 agcccaggat cttggattgg cgtgatcttc ggcactgaga agctgacggt aaatcatgac
     3961 tcagcggacg gacgcgctcg actggcgtta agcgcctgcc gaaggagcaa tcatggctga
     4021 cgaagtcatt gactcctcgc gtattgaagc ggcaaaagaa atcgcccagg aagaagaagg
     4081 accgcgacgc cgattggccg gtcccctctc ttggcttgcc tatagtcttg ccgcggcgat
     4141 gtcgctggtt tttatttatt gggcttggac caccgtcacc acgcaaattc tccggctggt
     4201 ttttctcgcc tttacactcg ttctgacttt tctcctttac ccgcccacca aaaaagcccg
     4261 actcagaagc gtgatttggt ttgactggat cctggttgcc ctgagccttt tggccctcgg
     4321 ctacccgctg tgggatttcg aggaattcat ctaccgagcg gcattgccgg atcttaaaga
     4381 tcaaattttt ggcgcgctca cgatcgtgct ggtgctggaa gccacgcgcc gcacaagcgg
     4441 ttggattctc ccggtcgtag cattggtttt tctggcttat gcctggctag gcgcttggtt
     4501 gccatcgccg tggactcatc agggctacgg attggaccgt ctggtcggtc acatgtacat
     4561 gaccctggaa ggcatcttcg gtgtaccgct cgacgtcgcg gcgacgttta tcattctttt
     4621 taccatttac ggcgcggtgc ttgagcattc gaaagcgggc gagttcttcg tgaatttctc
     4681 aatggcggca actggcggaa aacgaacggc ggccggcaga accgtcaccc tcgcatcctt
     4741 tttgctcggc gggccgtcag gatccggtgt ggcgacgacg attacgctcg gctcggtagc
     4801 gtacccgctg ctggcaaaag ccgggtatga caaagattct gcgggcggcc ttttgtccgc
     4861 cggtggaatc ggcgcgatta tctcgccgcc ggtgctaggc gccgctgcct ttctgatcgc
     4921 cgagtttctc cgcatctcgt atctcgaagt cattgcgttg gcggcgatcc ccacgttgct
     4981 tttttactgg tctatttttg tcatggtcga gctcgacgcc cataagttcg gcgcacggcc
     5041 ggtgacggtg gaaacggaag gcgtctggag cctgacgcgg aagtacggtt accatttcat
     5101 ttcactggtg gcgatcgtca tttttatggt ctggggctac accgcgattt cgtcggtggt
     5161 ttacgcgatc ctcgtctcgg tcgtcttgag tttcttgcgg cccgagacgg cgctcactcc
     5221 gcctcgtctg tggcgcgctc tgatcgacgg cagcacaggg gtgctatccg ttgccgccac
     5281 ttgcgcgacg gcaggaatta tcgtcggcgt ggtaacgctc accggtctcg gactcaagtt
     5341 ctccatgatc atcttgacaa tggcgggagg caatcttttt ctcaccatac tctataccgg
     5401 cctggccgtt tggattctcg gcctggcgat gccggtgacc gcctcttaca ttattgctgc
     5461 agtcatcacc gcgccggcaa tgatcaaatt gggcgtgccc gactatgccg cgcacatgtt
     5521 catcttctat tatgccgtgc tctccgaagt gtcgccgccg acggcgctct cgccgtttgc
     5581 tgccgcggca attaccggcg gtaatccgta caaaaccacc atgatggcat ggaagtatac
     5641 gcttcccacg ttcttggtgc cgtttatgtt caccttgcat ccggacggcg ccggcctgct
     5701 tctcaaaggc ccgtgggaga atgtcgtttg gacgagcgtg accgcggcgc ttggattggc
     5761 tgcgctcgca gcgggactta ccggttggat gctgatgaaa acgacttggc tggagcgcgg
     5821 tttattgctc gccgccgggt tggtattggt ctacccgagt ctgattcagg acgtcatcgg
     5881 cttggtcctg tttgcgtctg ccgggtccct gcagtacttc cgccgccgct ccggcggtga
     5941 aaccttgccg gcgcactaaa aggcaaaagg tcctatcgtg tggccatagt gatcttaatt
     6001 tgcgatatct tttgatatcg acgcgttcgt aaccgaaacg gattccggga tcaagaacca
     6061 atcgagttgt ttattcggcc gcgccgcaca tcgaattcga cacgctcgac tcgcccggca
     6121 attcgagccc agttctcgat gatttgcgtc atttgctagc gccgtctcgg cctttatccc
     6181 gtcgcattcc gtttggctgc tgtccggccg gcttggttcg tgggtctcac tgttctcggc
     6241 agagcgatgc cggacgcacg tttgacgtgc acagtggcct tgatgtcgcc accgacgaga
     6301 taacgaatga agtattctca tgaggcgtgc ggtcgttcat tggattcctc aacatgaacc
     6361 gatccgacgg tcgttcaggg ttgacgccat gctttagtcc ttgggcagcg atcggcgcgt
     6421 ggtgacgctt gcagccgcga cgcatatcga agatcgaacc gaatattatg cgtcgttgat
     6481 tttctgacta ccgtccttca tgtgttcggg taacccgaac gcctcgcctt caacgcaacc
     6541 ggttcatggt tcttctttta gcgggccaaa agcttttggc gagcgccggt gcttggttcc
     6601 ggcttcatag gccgatgtga tctcgaacag cgtcgcttca tcaaacggac gaccaagaaa
     6661 ttccatgccg actggaattt gatcgggttt ggggccgtcc aatcggctgc gattgggatc
     6721 gttggcgtcg gggatacggt cgtaaacttc tcgggtgaaa cccgccggca caacaatggc
     6781 ggggaagcca cttaccggac tcaagttcac gctccaactg ctgccggcgc cgcgccacag
     6841 atctaaatcg tttttcagtt tggtctcgcc cggcaataaa ttcggatcgg aaagttccgt
     6901 gccggccttc aatacccgtg gttcggtgtg cgtgtcatag acttctgggg tgcggttggc
     6961 taacaccgta tgcggcggaa tggtgctgta gggatacacc agcgcgtcga ggttattatc
     7021 cgccatgact ttgtagagaa tccgttgaag gttttgcatg cgtaagatgt ggctcgtgtg
     7081 cgccggagtc tcgagggttt ttgccttggc gccaaatgca ttctgcagca ccggcaggtc
     7141 gccggagaag gttttggtcg cgaacatgtc ttcgacgctg cgaaatttgg catcgccgcg
     7201 ctggcgtaaa taaagattca tcgcgtactt gccttcttca ccgcggcgct gcccggcgat
     7261 catgcgcaga ttaacgccgc gcataccggt cggcagcgtt ttcggatcat cgctgatttc
     7321 gaccatgcga tcgatggggt cggatccgga cgcgacggcg cttttgaaag tctgcgtgaa
     7381 gaagctcggt tcataagacg ccatgatctc ggcgatggcg ccgtcaaaat ttaccggatc
     7441 gacgatggtg gcgccgagac gcttcatgtc ggcgatcgcc tgattggcaa tgcggatgct
     7501 gtcgcggtcg gcgatggttg cttcgatcat gaactcgcgg actacgccta aacgtttacc
     7561 cgcaagcgat tttctgccgg cgtaatttcg gtacggcacc ggttttttac catgcgccgg
     7621 cgccgttaca tgatcgcgcg ggtcggcgcc cgcgacgact tcgagcaccg ccgcggtatc
     7681 ctgcacggtg cggcacatcg gtccgccgcg gtcgcgcgag aatgacaaag gaatgattcc
     7741 cgcgcggctt accagtccct gcgtagcgac catgcccacc agcgcattcg cggtggatgg
     7801 aaaacgcacg gagccggaag tgtccgtgcc gagcgcgcac atcgccagat tggccgccac
     7861 ggcagcgcca gacccagcgc tggaaccacc cggaacgcgc ttggtgtcgt atgggttgca
     7921 agtctggccg ccgagcgcgc tgcgcgcgat gctggccggc gcgtactcat ccagattggt
     7981 cttgccaaga aagatcgcgc cggcggcgcg aagctttgca atcatcgtcg catcctgcgg
     8041 cggtttatcg ttggcataat ccgcggctgc gcccgcggtg gtccgcatat ccacggtgtc
     8101 gtagttatcc ttgatcgcga aaggaatacc atgcagcgct ccaaccaatc tgccggtgcg
     8161 cgcgaagtgc gcgtcctgag ccttggccac ttcgagcgca tccggcaatt tcggatcgtt
     8221 atcggcgcta tcggtcttgg aacgtttacc gcgaatattg agggtcatat aagcgttcag
     8281 ctgaccggca ttttcgatcg gtgtcagatc accaagcatc agtccggtgg caggatcgac
     8341 gtcacctttc acacagatgc cgttataagc ctcgatgcgc ttaaaataga attgcacgag
     8401 ttgggtcgcg gtgatttgtt tggcgcgaat ggcgcgatgc acgtcggcaa tggtcgcctc
     8461 ttcgagatga aagcgactcg agggtgcgcc ggccgcacgg acattgccgc gccctaaggg
     8521 gaacagcatg gaaccgatac tcagcagcgc aatgcttcga aacgataatc gaaaattcac
     8581 aacacgtcct cctgcgattt gcttagcgaa cactagcctc agcgagagta gctcgtcaat
     8641 gaaaattatt tcgccgacag tttcgaggta tagcagttga gggagagtgt aaagttaaac
     8701 gtcgtcaatc gattcaaggc atgcagaatt gaattcgaag tagatgcgga actggaggat
     8761 gacgttgcaa caaacttgat tcggcgatgg gtcttggacg aatactcgag ggacgattgc
     8821 tttaactcta tttagttttg gccctttgcc cgcgccgcca tccatgcggc gaccagggct
     8881 ccgatgacgg ccgcaccgtg cactgccgcc atcggcaccc aggtgccgtc ggcgagcagg
     8941 ccggtgagct gctgcagcgc cgctggccag aaaaattgcg agaacacgac cgcgccggac
     9001 gccgagccgg acagctcggg atcgaccgcc atcgcgccgg cttgagcata gggcatggag
     9061 agaccctggg cgaggctcac gaaggtgccc ggaatgtaga gcgcggccat ggagatgccg
     9121 ccgaagtaaa accaggcgac cagcagcgcg ccgttgacca cgccgattac gccaccgagg
     9181 atggtcatga attcgatgga gcgtttcgca ccgatgcggc ccgagataaa actgccggcc
     9241 atgaagccga tcggaaaggc gaagaaccac cagccgatct tggcaggatc ggcgccgaga
     9301 tgctcggcgg cgagaaacga ggccgctgtc gcctgagtgt aaaaggccgc ggacatcagt
     9361 cccggttgca gcatgaaacc gacgaaacgc aggcgccgga gcagcgactt gtaaccggca
     9421 aatacgctcc gcggcgccgc ggcgctctga actcgagttt ccggcacacc aaagatcacc
     9481 gcgaagatca ctaacaaccc gaccacggac gccaacacga acagcgcgcg ccagccaaag
     9541 gcggcggtca actggccgcc caccggcggc gcaaacatcg gccccagcac gtaggcggca
     9601 gtgagataag cgatcacctg ggcgacgcgc tcctggccgt agacatcgcg ggcgatggcg
     9661 cgcgccagca cgacgccgca tcccgcgccg gctccctgca ggatgcggcc cgcgagcagc
     9721 atcggcatat tcggcgcgac catgcaggcg gccgcgccgc aggtaaacag cactaacccg
     9781 gctagaagca cgcgcttgcg gccgaggcgg tcggacaaac cgccataggc caccgtaaag
     9841 aaggccatcg acagcatcgc gagcgacatc gtgagctgtg ccacgccggt cgagacgtcg
     9901 aaggcatcct tcaccgccga catggccgga atgaacagat gaagcgagag cggcccgatg
     9961 aaggtgacgg aaaggagcgc gacgaaaaaa taccagcgcg ggcggaacgg aatggtcatg
    10021 tttgtactaa tcacagaatg gttgagacag atctagtttc catcgtcgtt cagccgccgg
    10081 cgcgcccacc atctcaattc acccggatta gctctaatga atctcgccgg ccgggatctc
    10141 gctgaccaac tattgaaggg cagtctttta tttcggcgct cttgcccgcc aatccagcat
    10201 cggagcgcct aatcggattc gattctctgc cgcttatccg ctgttggctg aaatcgcgcg
    10261 atcgctgcgt tttttcgacg cgcagctttg ccggcccgtc ggttttacca gatccaaccg
    10321 tttttgtcct atccatcgag ggacgcactt tgcgctctac tgcggtcaga ttattcaaaa
    10381 ggcggcaagc agaatcacga atagcggtct cccgggcgta ttaagctgta aattctcttg
    10441 actagtctac tgatgaacaa gtaggaacag aaagtccatg ccccggaggt atttctggcg
    10501 atgatgaaaa aattatcgat tttagccggc gtgcttctgt tcgctttctc gagcgctgta
    10561 ggagcgcagg aaaagatccg catcggcatc ggttcggtga gtctgcagtc ggtgctgccg
    10621 tttatcggca agcagcgcgg aatttttgcc aggtacaacc tcgtccctga aattatctac
    10681 attcccggcg gttcgaccaa tgttcaagcg ctgatttccg gtaatctgga tctttcacag
    10741 ctctcaggcg cacctggagc cgcggccaat ctagagggcg ccgacattat ttacattctc
    10801 ggcttgttgg ataagttgaa ctaccaactg atcacacgac cggaaatcaa aaatatcgaa
    10861 cagctcaaag gtaaaagatt cggcatcagc aggtttagct catcggcgga tttcgggctg
    10921 cgcgccttgc taaaaaaagt cggcgtcgat ccgattaagg atgttacgat acttcaaatt
    10981 ggcgatgagc acgctcgcct cgcagcggta aaggccggta atatcgacgg aaccgtcatg
    11041 aatgcgccgt ttggcgcgga agcagaaaaa ttgaaactga atgtcgtagc ggattcggtg
    11101 aagatgggta ttccattctt caacaccggg ttgctgggta gcaagaagtt tttcgatcag
    11161 aacgaagcaa aagtcctcaa cttccttcgc gcctaccttg aatcgataaa agttttcaag
    11221 accgaaagag aatacgccgt caaatcgacc gctcagttca ctcgggttaa caacattaaa
    11281 gcgatcgagg aaggatatga ctatttcaaa gagcagcttc gcaatgtgcc gtatccgtca
    11341 gcggaggcga tgcaggcagt ggtcgcgcaa ttagccgaga caaatgtcaa agcgaggaaa
    11401 atcgatggca aagcttacgt caacgatcgt tacctgaggc aactcgaaga tgaaggattc
    11461 gtaaagaagc tctgggagag caagtagtac cggtaacaaa gagatttgcg attggaaaga
    11521 cacaggagga aagagcacat gctggcgatg gtctcgtttg ttttcatctt cttcctgtct
    11581 catgctgctc caggggccga agccgacgcg caacgtcaat tagtcgaggg cgccaagaag
    11641 gaaggcaagt tagtctttta tacgtcggtc gaaaccgagt tcgcgcggct gttgaccacc
    11701 ggcttcgaag caaaatatcc gttcgtcaag accgacatct tccgctcgaa tcacgagcga
    11761 atctttagcc ggttgaacgt cgagcgaaag gccggcacgt tcgccgggga tgtgctcagc
    11821 gtcggcgaat tcgaaactta ccacatgcaa aagcgcgggc tgctgacacc ttacaaggcg
    11881 ccctccgccg ccgtttaccc cgaaggattc aaggatccgg atggttactg gacggatttt
    11941 tacgacaatc taattgtcag cgcttacaat accacgagag tcaagcgcga agagctgcct
    12001 agaaattatg aggatctgct acatccgcgt tggcgccgcc gcatggtgct tgatcgcaac
    12061 gaggatcgct ggtttgccaa catgcttcat catatgggag aaaaaagagg catggagttc
    12121 atgcaggcgc tggcgaaaca agaagtggcc atccgctcgg ggcgttcgct gatcacccaa
    12181 ctaatggccg cgggagagtt cgacttgcag atcgttgcct actggtatcg gccctatctg
    12241 atgaataaac aaggagcgcc ggtagattgg ttttctctgg agcccgcatt cgtcgcgctg
    12301 catccgatca gcatcatcga gcgggcgccg catttaaaca cagcgaagct tttcattgaa
    12361 tatgtgcttt ccgaagaggg gcagcgcttg ctggcaaaac ggggacggga gccggcacgt
    12421 ccgggtatca ggccggaagg atatcc
//
LOCUS       DASUVS010000391        13698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1701130, whole genome shotgun sequence.
ACCESSION   DASUVS010000391 DASUVS010000000
VERSION     DASUVS010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13698
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701130"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VF182_20865"
     CDS             complement(<1..540)
                     /locus_tag="VF182_20865"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6439581.1"
                     /translation="MKLHILGCGDAFGSGGRNQSAYLIDDPDRVFLLDCGATSLLAMK
                     KLGFDSGRLDVVLLSHLHGDHFGGLPFFFIEYLYEKSREKPLHVAGPVGTEEKVLNLF
                     DLMYGRGTKSEALTTTCFHVLEPGKTSTIEGVQVFPFRVPHQVNDVSLALKVSYKGKH
                     ILFSGDSAWTDAFIEHAHAV"
     gene            738..1145
                     /locus_tag="VF182_20870"
     CDS             738..1145
                     /locus_tag="VF182_20870"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX6439582.1"
                     /translation="MRFLRFNLSSHKKLEFDLGKVGLTPPQFYVLATIGYAGSLPFGE
                     IGAKMMVTVSNLTGIVDRLEQKKLVLRKRDGTDRRVVHVTLTEKGASLYKTAIPLFER
                     SVSEIFSSLDLPRQKELSALLRNLNHADYPALP"
     gene            1237..2460
                     /locus_tag="VF182_20875"
     CDS             1237..2460
                     /locus_tag="VF182_20875"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX6439583.1"
                     /translation="MHDESNKKLGLQRVKKWLYAKGLDERSSGCVLCGSCYGHGPANP
                     MEDIPGPKEKCPPYEFYRFQRHTPKSRWLMAQRVFHGLDPITPELKEIIYSCTSCLMC
                     QELCGVRNDGYGPWDITVAMREQITEEEGPIDAHRRLYQNLRQYDNPWSESRAERGSW
                     ADGLGLNKIPHRGAPTLLFAGCSADQASGRNGVVALAKLMQKAGEDFAILGDQEKCCG
                     LHAYDLGFRREYDRLQQENLAIIAKAGVRTLVITCGSCQRMWREHVKVDGTDFKVLHG
                     VEYLQAALQAGRLKITTPLRKRVTYHDSCHLGRGCGIYEAPRAVLRAIPGLEFVEMKR
                     NQRWAWCCGGGGGVPEADPDLARCSASDRLREAKETGAELVLTSSALCQRSFKALEQP
                     ALPIQDLIEFVGQAV"
     gene            2463..3197
                     /locus_tag="VF182_20880"
     CDS             2463..3197
                     /locus_tag="VF182_20880"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439584.1"
                     /translation="MLKEAHFTPQKMLSMLGLKADSLGPYALVPGPKERSQLLLAMLD
                     QPQKNFTFLDYEMHTGTLDGKRVTVGNGGRYAPDTAITTEILCGAGAQALIRVGSCGS
                     LQEHVKIGDLIIVTGALRGEGTSPYYVAQSFSTVAHPEIVEALKRAAASLQVRFHVGW
                     IFTTDALFQETPELIAQLTQQNISGIDMVTSVFLTVAQIRKKKAAAILAVSDECLFGK
                     FGFRDPLFLKAEEEIIAVAREALKYF"
     gene            3221..4732
                     /locus_tag="VF182_20885"
     CDS             3221..4732
                     /locus_tag="VF182_20885"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439585.1"
                     /translation="MTSVQSPLFEPVLWERGGFRILDEVCLPEQIRYITVTDVSQAIN
                     AVREMKTRAFGQVLTFLYSGALLAQNYHRQEILPLRESIDRMTEAFCRARPTFDFDGL
                     CQHFSEWFDKLPEGVGAGEWISERACDFAAQIVRARGARAKRVAELLPDEARVLTHCN
                     MSGELLATAQHCAVMGKEFAVIATETRPYLQGARLTAWELARAGVRVSLIPDCAIAQV
                     MAKGEVNAIVVGSDRAAQNGDVINKVGTYSLAIMAKQYGVPFHVLVQDPRSLAQGSDV
                     AIEERPGSELLTFQGHQLIANNSIAVRYPAFDVTPGALITNFIGFDDVYTPESFRRKY
                     QRRAAATTSTASESKYLLVYGIPPLDQGDHLIGALKIEQASRFLIPEMRPQMWGVHFV
                     APALLKHDAPTTLISDNMMGIFFARGEVRKLYLYHSLLDGQGAMGICGSLLALGLARA
                     HAVPVELLASDSVKRSTVDTDVSTFMGKTICPPGASVFPLEREVMPLEMLKRA"
     gene            4741..5745
                     /locus_tag="VF182_20890"
     CDS             4741..5745
                     /locus_tag="VF182_20890"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6439586.1"
                     /translation="MMKKLQGEMIKWGKLLFDRHLISGWGGNLSCRIGENEFLITGRH
                     APLGFLTTEDLVQIDGSGRPLNGEHPASSETPLHLAVYAATGTDVIVHVHPPMVLAFS
                     LTHDHFVPVSFEEKYTIGEVPIIAQDTPTVTKPERFAEELKYRPVGIIKGHGTIAIGK
                     NFQEAFLLTDLLEEAVRCRFFTNIRGESDVLIADQRKRQEGLVETQTGKSYVLFSEEH
                     MSALVHRANHDSQFRSQGEETGLSTSLTLFLEDRAAAWTVYFDRGEITHLDSDDRGEF
                     LISGTEEWWSAVFGARIDPFLATQHGKLKLERGELAQLARWYKPFQRAFSLWQTILIQ
                     "
     gene            5721..7250
                     /locus_tag="VF182_20895"
     CDS             5721..7250
                     /locus_tag="VF182_20895"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6439587.1"
                     /translation="MANHIDSVGFFAAELLDQAARQHPNKIAVIDKEEALSFAVLNRR
                     VHALAGHLQSEGIRQSDRVGVLSPNCAAIPLIYFASQKIGAVNVLLDARLRGKELQEV
                     LRDADLKLLVIHQQLSSETITVLEELTRVPVWIVQGGGEEAFENRYAAPPRSALSPRP
                     NPDDDALILYTSGTTGEPKGVVLSYRNLAQYPRVMTEMGITDSSTIRGCILPMSHIVG
                     PLVCNEVADKGFTLVIFDQINPVTLLEGIQKYRISVFESVPIIFQLLLGVRNLSNYDT
                     SSLKIAAMMGTTVPLPLLRAFHSAQPHIAVIQGYGLTETSPMITLVEPDKAEAKIGSI
                     GRAVPGIEVKIIDKFGREMPIDEPGEIITRGPHVMKGYFRKPEATAARIRDGWLYTGD
                     VGKRDSDGYLTHLGRVDDMIITSGLNVYPAEVENLIYSFPNVQETVVFAIPDPKRGQV
                     IGAAVVPRPGKTIAAKELLIFLRGNLANFKVPQKIAIRDSLPRTSSGKVIREPDTLLS
                     N"
     gene            7396..10077
                     /gene="polA"
                     /locus_tag="VF182_20900"
     CDS             7396..10077
                     /gene="polA"
                     /locus_tag="VF182_20900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX6439588.1"
                     /translation="MNAKIKNQASRKAPGSSKDARSRDNLRKIILIDGHSLAFRAFFA
                     LPDTLITSSGQVTNAVYGFTSMLIKLLADERPQAVVVCFDKGAPQFRLDRYAAYKAGR
                     AETPDLFRQQLPLIREVLQALRIPIVELEGYEADDLLATLTKNCRGEGCEVIVVTGDR
                     DILQLVGDGVSVIMTRRGISDVIRYDAPTVTERYGVAPDQWLDFVALKGEPSDNLPGI
                     PGVGDKTAAQLVRKYGDIEGIIAHADELTPKLRDSLKAHAEQVRINKELGRLLSDVAL
                     ELDSVKLCLERWDDEEVRKLFTSLEFRSLHERLRALNMHISAPMAAALELKSISDLTS
                     ADEIKREGAAALAWNENWLAVCDGPGETHTLGTSAAIHRLADFFQDPARLKVAHDAKS
                     IYRLALGGGIEIHSLYCDTKIAAYLLEPGSASGYKLADAVKRYLGVSLNGNTSEPEIG
                     EQSDLAFDADDKERIAREAVAVRSMVPILQQQLKDQGIWELASTLEFPLVGVLARMEH
                     NGILIDRDYLEKFDAELGNKVIALERAIKDFAGEDFKVNSQPQLQRILFEKLALPKTR
                     KIKTGYSTDAAELEKLADVHPIIRVLLEYREVSKLKSGFTEMLLELIDRKTNRIHTTY
                     EQTTAATGRLSSTAPNLQNIPIRGELGRQIRRAFIAPPAHVLLSADYSQIELRVLAHL
                     SQDETFLQAFAQGHDFHATIAAKVYGIALTDVTSQMRNQVKQFSYGIAYGMSTFGVSQ
                     RLGVAMDVAASFVEAYYAQFPKVKEFLAAQVNQAKIDGFTTTMFGRRRYLPELGSSNF
                     RLRALGERMALNTPIQGSAADIIKKAMISVDEALRKEPVAKMLLTVHDELVFEIPVEH
                     LDLARALIEQAMTQAADLRCGLVVDIHVGPNWAEAHA"
     gene            10153..10944
                     /locus_tag="VF182_20905"
     CDS             10153..10944
                     /locus_tag="VF182_20905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439589.1"
                     /translation="MAFSQRTPLLILVILLVLPPAWIGSALGQSGNTRQTSKVSRGGA
                     ADAELARLRADVIQKMRDSRAGAEKLIALREKEVAKLTEEYGKRRQFYHQGLISKAEL
                     DQSERSLAEAIARLQDDKRRLGEQDIAITEALMRGEELQSPGLAVDGYSETGSLIRFN
                     GNGAWTIAEAPKIQNFFFQTFGRALPISAYGQTVIHDRLQFDHRNAVDVALHPDSQEG
                     RSLIFHLRQSRIPFIAFRNAVSGSSTGAHIHIGRPSPSLLTAMRR"
     gene            11009..11728
                     /locus_tag="VF182_20910"
     CDS             11009..11728
                     /locus_tag="VF182_20910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439590.1"
                     /translation="MTEFVVPGKASTVIVARPGAGGRFEVLMTRRSPHLKVLAGFLVF
                     PGGAVEEDDCSEKMLARCRGLSPIEAQRILAATMAPEQAMGHWVAAIRELFEETGIHF
                     FAKQGESLDPISDSLRERLAESREALWQRRLVLADLLESEQLFCDVGRLTYLFHRITP
                     NHHPVRFDTRFYVSFLPQGQSPLNCSEEVAESVWLVPQEALKLSESGVFPMMPPTVIA
                     LRALAELGSWKNFISAYRLKV"
     gene            11864..13591
                     /gene="glgP"
                     /locus_tag="VF182_20915"
     CDS             11864..13591
                     /gene="glgP"
                     /locus_tag="VF182_20915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017838793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX6439591.1"
                     /translation="MMTEPTAFSVPATIAYFSMDVAIDSDIPTYSGGLGILSGDTLRS
                     AADLGVPMVAFTLLHRKGYFDQRLDAAGNQLESPSHWSPEKFLEPLPPRVSVLIEGRE
                     VKIRAWQHRFNGVMGHTVPLLLLDTDLEENDSRDRALTDHLYGGDDRYRLCQEAVLGL
                     GGVQMLRALNYPGFKIYHMNEGHSALATIALLEQLSANRRFSETEIETVRRQCVFTTH
                     TPVPAGHDRFSAELVHQVLGENRDALLRQLGVMDGSLNMTELALRLSGYANAVSMRHG
                     EVSRHMFPGHRVEAITNGVHAEAWITKPIATLFDRHVPDWRRDNCYLRHAIGFPPQQV
                     REAHGEAKRELLQQVRWLTGSQLDDAVFTLGFARRATAYKRGDLLFTDLERLKNIARQ
                     VGPLQLIYAGKAHPRDEGGKAIIRRIFEAAAALANDVRVVYLENYDMALGKMLCAGVD
                     VWLNTPLRPQEASGTSGMKAALNGVPSLSVLDGWWIEGHIEGVTGWSIGELGSGDNDT
                     NAEVNSLYQKLGDVILPLYYNAPDKFAHIMRSAIGFNGSFFNTQRMMFQYLRNAYLPA
                     WTKAQASLL"
BASE COUNT         3153 a         3564 c         3802 g         3179 t
ORIGIN      
        1 gaccgcgtgg gcgtgctcaa taaacgcatc cgtccaggcg gagtctcctg agaacaaaat
       61 gtgcttgccc ttgtaactaa ccttcagcgc aagcgatacg tcgtttacct gatgcggcac
      121 gcggaatgga aatacctgaa ctccctcaat agttgacgtt ttcccaggct ctaaaacgtg
      181 gaagcaggta gtggtaagag cttcggactt ggtccccctg ccgtacatga ggtcaaacag
      241 gttaagaacc ttttcttcgg ttcccaccgg cccagccaca tgcagcggct tttcgcggct
      301 tttttcgtac aagtattcga tgaagaaaaa aggcaggccg ccaaagtgat cgccatgcaa
      361 gtggctaagc aagacgacgt cgagtcggcc ggaatcaaat ccaagctttt tcattgcgag
      421 cagggaagtg gcaccgcaat cgagcaagaa aacgcggtcc gggtcatcga ttagataggc
      481 actttgatta cggccgccgc tgccgaatgc atcgccgcag cccaatatat gaagcttcat
      541 tgctccctcc gaaatctatc gtccagttaa gccgctggtg tttacgagtc aaggcgcgga
      601 agcgctgacc cggtatccaa ccctcccata gggcgacccc gagctatttc attgcttttt
      661 gatttcccgg cgactaaatt gaccaggtgc cagtccacaa gcgccccttt tttgcctcct
      721 acagcacgag tctctgggtg cggtttctac ggtttaatct ctcgtcgcat aaaaagctgg
      781 aattcgacct tgggaaagtc ggtctaacac cgccacaatt ctacgtcctc gcaactatag
      841 gttacgccgg gagcctcccg tttggcgaga tcggcgctaa gatgatggtc acagtcagta
      901 atctaaccgg tatcgttgac cgcctggagc aaaaaaaact cgtcttacga aagcgcgacg
      961 gcaccgaccg ccgcgttgtt cacgtgacac tgaccgagaa aggggcatcc ttatacaaaa
     1021 ccgcaattcc tctgttcgag agaagtgtct cggaaatatt ctcctcgcta gatctgccgc
     1081 gtcaaaaaga gctctctgcc ttgctgcgaa atctgaatca cgccgactat ccggcgttac
     1141 cttagtttgt tgacacaccg gtttgttcgg cgctatacaa ttaaagcctt aagcatttag
     1201 tcccgaggaa ttgcagtcta atacctccgt catactgtgc acgacgaatc aaacaagaaa
     1261 ctcggcctcc agcgggtcaa gaaatggctt tacgccaaag gtctagacga gcgcagcagt
     1321 ggctgtgttt tatgcgggtc ctgttatgga catggtccgg ccaatccgat ggaagacatt
     1381 cccggcccta aagagaaatg tcctccctac gagttttatc gtttccaacg gcacaccccc
     1441 aagtcgcgtt ggttgatggc gcagcgtgtc tttcacgggc ttgacccgat cacaccggaa
     1501 ctgaaagaaa ttatctacag ttgtacaagc tgcctgatgt gccaggagct ttgcggcgtg
     1561 cgcaatgacg gttacggccc atgggatatt acggttgcga tgcgggagca gatcactgag
     1621 gaagagggtc cgattgatgc ccatcgccgg ctgtaccaaa atttgcggca atacgataat
     1681 ccctggtcgg aatcgagggc cgaacgcggt tcttgggcgg acggactcgg gcttaacaaa
     1741 attcctcacc gcggcgcgcc gacgctgttg ttcgccggct gctcggccga tcaagcaagc
     1801 ggtcgcaatg gcgttgtggc cttggccaag ctgatgcaaa aggcgggcga ggactttgcg
     1861 atcctcggcg atcaagaaaa atgctgcgga ctgcacgctt atgaccttgg atttcggcgc
     1921 gagtacgacc gtctgcagca agaaaacctg gcaataatcg cgaaggccgg agttcgtacg
     1981 ctggtaatta cttgcggtag ctgtcagcgc atgtggcgcg aacatgtcaa agttgatggc
     2041 accgatttca aagttttaca tggcgtggaa tacctgcaag ccgcgttgca ggcgggtcga
     2101 ttaaagatca ccacaccgct ccgaaaaagg gtcacctacc acgactcctg ccatctaggg
     2161 cgaggttgcg ggatttacga agccccgcga gccgttttgc gagccattcc cggactcgag
     2221 ttcgtggaaa tgaagcggaa tcaacggtgg gcatggtgtt gtggcggcgg tggcggcgtt
     2281 cctgaagccg atccggacct cgctcgatgc agcgcatccg atcgtttgcg tgaggcgaag
     2341 gaaaccggcg ccgaattggt tttgacttcc agcgctctct gccagcgctc atttaaggcg
     2401 ctcgaacaac ctgctctgcc gatccaagat ctgatcgagt ttgtcggcca ggctgtttga
     2461 caatgctcaa agaagctcat tttacaccac agaaaatgct ttcgatgctc gggttgaaag
     2521 ccgattctct tggtccttat gccttggttc ctggtcccaa ggagagaagc caattgctat
     2581 tggcgatgtt ggatcaaccg caaaaaaact tcactttctt agattacgag atgcatacgg
     2641 gaactctgga cgggaagaga gtaacggtgg gtaatggcgg gcgctatgct cctgacacag
     2701 cgattaccac ggaaattctt tgcggcgccg gtgcccaagc gctgatccga gttggaagtt
     2761 gcggttcgtt gcaggaacac gtgaagattg gcgatctgat catcgtgacc ggagctctcc
     2821 gcggtgaagg cacatcgccc tattacgtag cacagagttt ttccaccgtg gcacaccccg
     2881 agatcgtcga ggcattgaaa agagccgcgg caagccttca ggtgcgcttc cacgtaggct
     2941 ggatctttac aactgacgca ttattccagg aaaccccgga attgattgcg caactcacac
     3001 agcaaaatat ttcgggcatc gacatggtga cctcggtttt tctcacagtc gcgcaaattc
     3061 gcaagaaaaa ggcggctgcc attctcgcgg tgtcggatga atgtttattc ggtaagttcg
     3121 gatttcgtga ccctttgttt ctcaaagccg aagaggaaat catcgcggtc gcccgcgaag
     3181 ctctcaaata tttttagcca ttacacttgg cagatctgct atgacgtcgg tacaaagtcc
     3241 gctttttgag ccggttctgt gggaaagggg cggattccga attctcgatg aggtctgcct
     3301 tcccgagcag atccgctata tcacggttac tgacgtttcc caggcaatca atgcggtgcg
     3361 cgagatgaaa acgcgcgcat tcggccaagt cctcacgttc ctttatagcg gtgccctgct
     3421 ggcacaaaac tatcatcgac aggaaatctt gccgctacga gagtcaatcg atcgaatgac
     3481 cgaggctttc tgccgggcac gaccgacatt cgatttcgac ggtctttgcc aacatttcag
     3541 cgaatggttt gacaagctgc cggaaggggt cggtgccggt gagtggatca gcgagcgggc
     3601 ttgtgatttc gccgcgcaaa tcgtccgtgc ccggggcgca agagccaagc gagtcgccga
     3661 gcttctgcca gatgaggcgc gggttctgac ccactgcaat atgagcggag aactgctggc
     3721 aacagcgcag cattgcgcgg tcatgggcaa agagttcgcg gttatcgcca cggaaacccg
     3781 gccgtacctg cagggcgcca gactgactgc gtgggagctt gcacgagcag gcgttcgcgt
     3841 ctcactgatt cccgattgtg cgattgccca ggtgatggct aaaggcgaag tcaatgcaat
     3901 cgtcgtcggt tcggatcgtg cggcacagaa cggcgacgtc atcaataaag ttggaaccta
     3961 ttccctggcc attatggcca agcaatatgg cgttccattc cacgttctgg ttcaagatcc
     4021 ccgttccttg gctcaaggga gtgatgtggc gattgaagaa agacctggct cagaactgct
     4081 tacctttcaa gggcatcagc tgatagcgaa caacagcatc gccgtgcgtt atcctgcctt
     4141 cgatgtcacg ccgggagcgc tgattacgaa tttcataggg tttgatgatg tttacacacc
     4201 cgaaagtttt cgccgaaagt accagcgaag agccgcggca acaacatcaa cagcgagtga
     4261 aagcaagtac ctgctggtct atggcatacc gccgttagat cagggggatc acttaattgg
     4321 tgcgctcaaa atagagcaag ccagtcgttt cttgattccc gagatgcggc cgcagatgtg
     4381 gggtgttcat tttgtcgcgc cggctctgtt gaagcacgat gcgcccacta ctctgatctc
     4441 ggacaacatg atgggaatct tcttcgcccg gggcgaagtc cgtaagctct acctctacca
     4501 tagcctgctc gacgggcagg gagctatggg catctgtggc tcgctgttgg cgcttggttt
     4561 ggcgcgcgct catgccgtac cggttgaact gctggcctca gattcagtta agcgatcgac
     4621 tgtagatacc gatgtttcga cgttcatggg gaaaacgatt tgtccccccg gagcctcggt
     4681 atttcccctt gaaagagaag tcatgccctt ggagatgctt aaacgagctt gagactacgc
     4741 atgatgaaaa agcttcaagg agagatgatc aaatggggca agctgctgtt tgaccggcat
     4801 ctaatcagcg gctggggggg gaatctcagc tgtcggattg gagagaacga gttcctgatc
     4861 accggccgtc atgcgccgct tggctttttg acgaccgagg atttggtgca gatcgatggc
     4921 tctggcaggc cgcttaacgg agagcatccc gcatcgagcg aaacgccgtt gcatttggct
     4981 gtgtatgcag ccaccggcac ggacgtcatc gttcatgttc atccgccgat ggtcctggca
     5041 ttttcattaa cccacgatca ttttgttcct gtgagtttcg aagagaaata taccatcggc
     5101 gaagttccta tcatcgccca agatacaccg acggtaacaa agcccgagag gttcgcggaa
     5161 gaattaaagt accggccggt cggcatcatc aagggtcatg gcaccattgc tatcggtaag
     5221 aatttccaag aagcgttctt gcttaccgat ttactggaag aagctgtgcg ttgccggttc
     5281 ttcacgaaca ttcgaggtga gtcggacgtg ttgattgccg atcaaaggaa acgacaagaa
     5341 ggtctcgtcg aaacacaaac cggtaaatct tatgtgctgt tctcggaaga gcacatgtcg
     5401 gcgctggtcc accgcgccaa tcatgactct cagttccgtt ctcaagggga agagaccggc
     5461 ctctccacgt ccctgacgct ttttcttgaa gacagagccg cagcctggac cgtctatttt
     5521 gatcgtggcg agatcacaca ccttgattca gatgatcgtg gtgagtttct gatcagcggc
     5581 acggaagagt ggtggagcgc ggtgtttggc gcccgcatcg atccttttct ggcgactcag
     5641 cacggcaaat tgaaactcga gcgcggtgag ctggcgcagc tcgcgcgctg gtacaaacct
     5701 tttcagaggg cgttttcgct gtggcaaacc atattgattc agtaggattt ttcgccgcgg
     5761 agctgctcga tcaagcggca cggcagcatc cgaacaaaat cgccgtcatt gataaggaag
     5821 aagccctaag ctttgcggtt cttaaccgac gggtgcatgc cctagccggg cacttacaga
     5881 gcgaaggcat tcgacagagc gatcgggtcg gcgtgctgtc accgaactgt gcggcaattc
     5941 cgctgattta ttttgccagt cagaaaatcg gcgcggttaa cgtgctcctt gatgcacggt
     6001 taaggggcaa ggagttacag gaggtcttgc gggatgcgga tctgaagctg ctggttatcc
     6061 atcaacagct gagctccgaa actattactg tcctcgaaga gctcacccgg gtaccggtat
     6121 ggattgtcca aggcggcgga gaagaggcat ttgaaaatcg ctatgccgca ccgcctcgat
     6181 cggcgctgtc gccgcgaccg aatcctgacg atgatgcgct tatcctttat acttcgggaa
     6241 ctacgggcga acccaaaggc gtggttctaa gctaccgcaa cctggcgcag tatccgcgtg
     6301 tcatgaccga gatgggcatt accgattcga gcacgatccg aggctgcatc ctgccgatgt
     6361 cgcacattgt aggcccattg gtatgcaacg aagtagcgga caaaggtttc acacttgtca
     6421 tcttcgatca aattaatccg gtcaccttat tagagggcat tcagaaatat cgcatcagtg
     6481 ttttcgaaag cgtgccgatt atctttcaac tcttattggg ggtgcgcaac ttatcaaact
     6541 atgacacgag cagcttgaag atcgccgcta tgatggggac caccgttccg ttgccgctcc
     6601 tgcgcgcctt tcattcagct cagccgcaca tcgcagttat ccagggctac ggtttgaccg
     6661 agacctcgcc gatgatcacc ctggttgaac cggacaaagc cgaagccaag atcggaagta
     6721 tcggccgtgc agttccggga attgaagtta agatcatcga taaatttggc agggaaatgc
     6781 cgatcgatga acccggagaa atcattacga gaggtcccca tgtgatgaaa ggatattttc
     6841 gcaaaccaga agccactgcg gcccgcattc gtgacggctg gctttacacc ggtgatgtcg
     6901 gcaaacgcga ttcggacggt tacctgaccc atctcggccg agttgacgat atgataatca
     6961 ccagcggcct aaatgtgtat ccagcggaag tcgagaacct gatctacagc tttccgaacg
     7021 tgcaagagac cgtcgtcttc gccatccctg atccgaagcg gggtcaggtg attggcgctg
     7081 ctgtcgtgcc tcggccgggc aagacaattg cggcaaaaga gttgctcatt ttcctgcgcg
     7141 gcaatctggc aaatttcaaa gtgccgcaaa aaatcgccat tcgtgattcg ctgccgcgca
     7201 catcttcggg gaaagtcatt cgtgagccag acacattgct gtcgaactga ttatcgcgac
     7261 ggctttctga aaaatagtgg aatcgccttc agccgcggtt agagcaacga tgccggtctt
     7321 agctcattcc tgatggccgc ggctcgaccc cgcgcatgtt tgctccaggt aaattcaacg
     7381 ggtagactgc taccagtgaa cgcaaaaatc aaaaaccagg ccagtcgaaa agcgcctgga
     7441 tccagcaaag atgcccggtc aagagataac ctgcgtaaga ttattctaat cgacggtcat
     7501 tccctcgcgt ttcgcgcctt ttttgcactc cctgacacgc tgatcacatc ttccggacag
     7561 gtgaccaatg ccgtctatgg gttcacctcg atgctgataa agctattggc cgacgaacgg
     7621 ccgcaagctg tggttgtctg ttttgataaa ggcgcgccgc agttccgcct cgatcgttac
     7681 gccgcctata aagcgggtcg cgcggagact ccggacctct tccggcagca gctgccgttg
     7741 attcgcgagg tgcttcaagc gttgcgaatt ccgattgtcg agctcgaagg ctacgaagcc
     7801 gatgacctat tggcgacgct tacaaaaaac tgccgcggtg agggctgcga agtaatcgtc
     7861 gtcaccggtg accgagacat tctccagctc gtaggcgatg gcgtttcggt gatcatgact
     7921 cgccgcggga tctccgatgt catccgttat gacgcaccta ctgtcacgga acgctacggc
     7981 gtcgccccgg atcaatggct cgacttcgtg gcgctcaagg gcgaaccctc cgacaatctt
     8041 cccggtattc cgggcgtggg cgacaagact gcggctcagc tcgtccgaaa atatggcgat
     8101 attgaaggca tcatcgcgca tgcggatgag cttacgccca aacttcgcga ttcgcttaaa
     8161 gcgcacgccg agcaagtgcg gatcaataaa gagctcgggc gtctgctgag tgatgtcgca
     8221 ctcgaattgg attcggtcaa actttgtctc gaacgctggg atgacgaaga agttcgaaag
     8281 ctctttacgt cactggaatt ccgcagcttg cacgaacgtt tgagagcgct gaacatgcat
     8341 atttcagcgc ctatggccgc ggcgctggaa ctaaaatcaa tttcggactt gacgtcggcg
     8401 gacgaaatca aacgagaggg cgccgccgcc ttggcttgga acgagaactg gctcgctgtt
     8461 tgtgacgggc ctggcgagac gcacacgctt ggcacgagcg cagcaataca ccgactggcg
     8521 gatttttttc aagatcctgc aaggctgaag gtggctcacg acgccaagtc catctaccgg
     8581 ttagccttgg gtggtgggat cgaaattcac tcgttgtact gtgacaccaa gattgccgct
     8641 tatttactgg aaccgggttc ggcgtccgga tataagctgg ctgacgcagt gaaacgttat
     8701 ctcggtgtct cgctcaacgg aaacacgtcc gaacccgaga ttggcgagca aagcgatctt
     8761 gccttcgacg ccgatgacaa agagcggatt gctcgtgaag cggtggccgt tcgcagcatg
     8821 gttccaatac tccaacagca gctcaaggac caagggatct gggaacttgc cagcaccttg
     8881 gaattcccgc tagttggcgt gcttgccaga atggagcata acggaattct catcgaccgc
     8941 gattatcttg aaaagttcga tgccgaactg ggaaacaaag taatcgccct tgaacgggca
     9001 attaaggatt tcgccggtga agatttcaag gtgaactcgc agccacaact tcagcggatt
     9061 ctcttcgaaa aactcgctct acccaagacc agaaaaatca agacaggcta ttccacggac
     9121 gctgccgaat tggagaagct cgccgatgtg catccgatta ttcgggtgtt gctcgaatac
     9181 cgggaagtat cgaaactaaa gagcggcttc acggagatgc tgctggagtt gatcgatcgc
     9241 aaaactaatc gaatccatac tacttacgag caaacaacgg ctgccactgg ccgtttgtcg
     9301 tcaaccgcgc ccaacctgca aaatattccg atacggggtg agctaggacg tcagatccgc
     9361 cgcgccttta ttgcgccgcc ggcgcatgtg ctgttatcag cggattactc tcagatcgag
     9421 ctgcgtgtgc tcgcgcatct atcacaggat gaaacttttc tgcaagcctt tgcgcagggg
     9481 catgattttc atgccaccat cgctgctaaa gtctatggca ttgctttaac tgatgtcacc
     9541 tcgcagatgc gcaaccaggt aaagcagttt tcttatggta tcgcctatgg catgtccact
     9601 ttcggagtat cgcaaaggct gggcgtcgcg atggatgtgg ccgcgagttt tgtagaagcc
     9661 tattatgcgc aatttccgaa ggtgaaggaa tttctagcgg cgcaagtgaa tcaggccaag
     9721 atcgacggat ttacgacgac catgtttggc cgtcgtcgct atttgccgga actaggctca
     9781 tcgaacttcc ggctaagagc gctgggagag cgtatggcgc tcaatacacc gattcagggc
     9841 agcgccgcag atatcataaa gaaggcaatg atcagtgtgg atgaagctct gcgcaaagaa
     9901 ccggttgcaa agatgctgtt gacggtgcac gacgaactcg tcttcgagat cccggtcgag
     9961 caccttgatt tggctcgggc cttgatcgag caagcgatga cgcaagctgc ggatcttcgc
    10021 tgcggccttg tcgttgatat ccatgtgggc cccaattggg ctgaagctca tgcttgaggc
    10081 taaaataatc gtcgagaaat cgacagtccg ctgtgtgaaa agcgctgaac gatggcgcgg
    10141 atctgagtac tcatggcttt ctctcaacgc acaccgctgc tgatcctcgt tattcttctg
    10201 gtactgccgc cagcctggat cggcagtgcg ctcgggcaaa gcggtaatac gagacaaacc
    10261 tcgaaggttt cccgcggggg ggcggccgat gccgaattgg cgcgactgcg cgccgatgta
    10321 atacagaaga tgagagactc acgggcggga gccgaaaagt taattgcgtt gcgtgagaaa
    10381 gaagtcgcga aactcaccga ggagtatgga aaacggcgcc aattttatca tcaagggctg
    10441 atttcaaaag cggagctaga tcaaagcgag cgctctctcg cggaggcgat agctcgcttg
    10501 caagacgata agaggcggct tggcgagcaa gatatcgcga ttactgaagc tttgatgagg
    10561 ggcgaggagt tgcaatcgcc cggcttagcc gtcgacggct acagcgagac cggttctcta
    10621 attcgcttta acggtaacgg agcctggact attgctgagg cgcccaaaat tcagaatttc
    10681 ttctttcaga ccttcggtcg cgccttaccg atcagtgcct atggtcaaac cgtcatccat
    10741 gaccgactac agttcgatca tagaaacgct gtggatgttg cgcttcaccc cgacagccaa
    10801 gaaggccggt ccttgatctt ccatctacgt caaagccgga ttccatttat tgcgtttaga
    10861 aatgctgttt ccggctcatc gaccggtgcg cacattcata tcggcaggcc gtcgccatca
    10921 cttctaactg ctatgaggcg gtgaagttct tgccatctcc gcgtacagat gccgggataa
    10981 cggctcacct cggatagtca agagtttcat gaccgaattt gtggttcccg gaaaagcttc
    11041 aacggtgata gtcgcgagac caggcgcggg tggacgcttt gaagttctca tgacccgtcg
    11101 ctccccgcat ttgaaggttt tggcgggatt tttggtgttt ccaggcggag ccgtggaaga
    11161 agatgactgc tcggagaaaa tgttggcgcg ctgccgaggt ttgtcgccaa tcgaggctca
    11221 gcgaatctta gccgcgacca tggctccgga gcaagcgatg ggtcattggg tcgcagcgat
    11281 acgagagctg tttgaagaaa ccggaattca cttttttgcc aagcaagggg aatcgcttga
    11341 tccaatctcg gatagccttc gcgagcgtct ggcggaaagt cgtgaagcat tgtggcaaag
    11401 aagactggtg ttggcggatt tgctggaatc ggagcaactc ttttgcgatg ttggtcggtt
    11461 gacctacttg tttcatcgga ttactccgaa tcaccacccg gtgcgcttcg acacccgatt
    11521 ttatgtaagt ttcctgccgc aaggacagtc acccttgaat tgctccgagg aggtggcgga
    11581 gagtgtttgg ctcgtgccgc aagaagctct aaaattatcc gagtcgggag ttttcccaat
    11641 gatgccgccg acggtgatcg cgctccgagc ccttgcggaa ctcggatcct ggaaaaattt
    11701 tatatctgct tatcgactca aggtataagt cttaacgata gcttagccgc acgaattgcc
    11761 gtggtttggc actcgtggta tcattctcgt gtaagacaag caatcgagtg acagcccccc
    11821 aattcttcaa acccagacta acgcggtaaa cgcaaagtta cggatgatga ctgaaccaac
    11881 tgccttttct gtaccggcga cgatcgcgta tttctcgatg gatgtggcga ttgattccga
    11941 cattccaacg tacagcggcg gtctaggcat cttgtccggc gacaccttac gctcggcagc
    12001 tgatttgggc gttccgatgg tcgctttcac actcctgcat cgaaaaggtt atttcgatca
    12061 gcggcttgat gccgccggta atcaattaga gagtccgtcg cattggtctc cggaaaagtt
    12121 tctcgagcca ttgccgccgc gtgtctcggt tttgatcgaa gggcgagagg ttaaaatacg
    12181 cgcatggcag catcgcttta atggtgttat gggtcacacc gttcctttat tgttgctcga
    12241 taccgatctg gaagagaacg attcacggga tcgagcactg acggatcatc tctatggcgg
    12301 agatgatcgt taccgcctgt gccaggaggc agtgttagga ttgggcggcg tgcagatgct
    12361 gcgtgcgcta aactacccgg gcttcaaaat ctatcacatg aacgaaggac attccgcgct
    12421 ggcgacgatt gccttactcg agcagctatc cgctaatcgt cgtttctctg agactgaaat
    12481 cgagacggtg cggcgccaat gcgtttttac aacacacacg ccggttccgg cgggtcatga
    12541 tcgcttttct gcggagctcg tgcaccaggt attgggcgag aatcgtgacg cgctgctacg
    12601 ccagttggga gtcatggacg gctcgctcaa tatgaccgag ttggcgctgc gattatctgg
    12661 ttatgccaat gcagtctcga tgcgtcatgg cgaggtctcg cgccatatgt ttcccgggca
    12721 ccgtgtcgag gcgattacca atggagttca tgcggaagcg tggataacaa aacccatagc
    12781 tacgttattt gaccggcacg tacccgattg gcggcgcgac aactgttatc ttcgtcatgc
    12841 gatcggcttc ccaccgcagc aggttcgtga ggcgcacggc gaggccaaaa gagaactgtt
    12901 gcagcaagtg cgctggctga ctggcagtca actcgacgat gccgttttca ctctaggttt
    12961 tgcccgacgt gcgaccgcct acaagcgtgg cgatcttctc ttcactgatc tggagcgcct
    13021 gaaaaatatc gcgcgacagg tgggaccctt acagctaatt tatgccggca aagcccatcc
    13081 gcgcgacgag ggtggaaaag ccattattcg ccggatcttt gaggcagcgg cagctctggc
    13141 caatgatgtc cgtgtcgtct atttggaaaa ctacgatatg gcgctcggga aaatgctttg
    13201 cgctggtgtt gacgtatggc tcaatacgcc gctgcgaccg caggaagcct ccggcaccag
    13261 cggcatgaaa gctgcgctga acggagtgcc tagtttgagt gttctagacg gttggtggat
    13321 cgagggtcac attgagggag tgaccggctg gtctatcggc gaattgggca gcggcgataa
    13381 tgacacgaat gccgaggtga attccctcta tcaaaaattg ggcgacgtga ttctacctct
    13441 ttactataac gcgccagata aattcgccca tatcatgcgt tcggcgattg gctttaatgg
    13501 atcctttttc aacacgcaac gaatgatgtt tcagtatctc agaaatgctt acctgccggc
    13561 ttggactaag gcccaagcgt cgttactata gttgtttgtc ccgcggcctt tgataatccg
    13621 aacaatgcgc gattaaacca tttcgttcgt ttccagttgt aaatgcaaat ccatcgctaa
    13681 ggtggttggt cggtattt
//
LOCUS       DASUVS010000392        32184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_827037, whole genome shotgun sequence.
ACCESSION   DASUVS010000392 DASUVS010000000
VERSION     DASUVS010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 32184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32184
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827037"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VF182_20920"
     CDS             <1..667
                     /locus_tag="VF182_20920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEX6439592.1"
                     /translation="QIYNRFKDYTMLLPDWYVANLELCENFGHIAGNIVECGVWKGGM
                     SAGIAYLLGDQRAYYLYDSFEGLPAAGDEDAIRGIVASAKSWEGKLKASQEHANEAMK
                     LSGAKRVTIIKGWFGETLSTYDAGPIAILRLDSDFYSSVMDSLINLWPHVVTGGLIIL
                     DDYYTWSGCTRAVHDFLSHNELNEPIQQFHETYAYVVKEADTNQSSRRCNKVEASVGV
                     QPH"
     gene            807..1325
                     /locus_tag="VF182_20925"
     CDS             807..1325
                     /locus_tag="VF182_20925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439593.1"
                     /translation="MSKILVVEPYKILQRAIAVSLFPEHEVEVTEQVPEMSALEQRSY
                     DAAIIDAGALREKNTAGDLNRCLQDWPIPIIWLEENISKAAPVRDQLVIVRKPIARND
                     LLAALARCQANGSTIPLNEAGEKLRNEPVLAGTEPRPDSEHRARIIELVDVVEEEPTA
                     GKQDKRLERKKP"
     gene            1329..4085
                     /locus_tag="VF182_20930"
     CDS             1329..4085
                     /locus_tag="VF182_20930"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX6439594.1"
                     /translation="MQLDKAYSHKLIEAKWYQHWTELGAFKPSKGDSPYFSMVIPPPN
                     VTGSLHMGHALNNTLQDILCRYKRMDGFRVLWIPGTDHAGIATQNVVERSIAAEGLTR
                     NQLGRKKFIERVWSWKQQAGNAILHQLRALGVSCDWDHERFTMDEGLSRAVREAFVRL
                     WDDKLLYRAQRLINWCPRCKTALSDIEVIHQESDGNLWHIGYPLAEDSSRELVVATTR
                     PETMLGDTAVAVHPEDERYQNLIGKNIRLPLSGRIVPLIADPFVDREFGTGALKVTPG
                     HDFNDFEIGERFALDKISIFDAEGRIDAGAFLQRGETGEWIDRYRGKDRFEARRLVVA
                     DLKERRFLRKIEPHKLSVGHCYRCQSVVEPYLTPQWFVNITPLAAPAIQAVRRGDIRI
                     IPEGWSNSYFAWMENIKDWCVSRQIWWGHQIPAWYCRSCDGENIVEGHDGEYSLTKEA
                     QPIVSRAPPKTCPRCGADDLVQDPDVLDTWFSSSLWPFSTLGWPEDTDDLKSFYPTST
                     LVTAFDILFFWVARMIMMGLKFMGDVPFYDVYIHALVRDEEGQKMSKSKGNVIDPLDI
                     MAEYGTDAFRFTLAAMAAMGRDIKLAEERISGYQNFVNKLWNASRFVLMNLAQSAAAK
                     QADEPSALDSTELGLADRWILSRLASTVRDARVAIDGYRFNDYANILYQFTWHEFCDW
                     YIEMSKLSLNGQFADDPERSRRMLVEVLEQILLLLHPIMPFITEEIWQGLGDGRETIM
                     LQKYPRAEADWIDAKTEKQMDHLMGVVRAIRNLRTELNCPPAKEIKVIFHGSDGDLAF
                     LDSHMPYLRALARVGSADFTPSGDRPKGAATAMVGATEIYLPIEDLLDLDEEHARLTK
                     EIAKISAEIARTQKKLGNPEFVNKAKAEIIQKEREKAIQHAEKLRTLEASLDQIQEIQ
                     AGRN"
     gene            4090..4944
                     /gene="nadC"
                     /locus_tag="VF182_20935"
     CDS             4090..4944
                     /gene="nadC"
                     /locus_tag="VF182_20935"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX6439595.1"
                     /translation="MDILVSPQIERLIRDSLDEDIGAGDLATMATISAESQGTGLFRA
                     KKQGVVAGLVLLERIFYFIDPKVSVRLLTKDGTQVAEGTVVAEAEGPVRALLLAERTA
                     LNFLQRLSGTATLTRQYVEAVKDFPCKVLDTRKTTPGLRTLEKYAVRMGGGTNHRIGL
                     YDAALVKDNHIEATGSIAEAVKAVRLHAPFMAKVEVEAGNLTQVQEAIDAGADVIMLD
                     NMSVAQMAEAVRLVNKRAWVEASGGITLNTIRQIAGTGVDFISSGALTHSAPVVDFNM
                     KITMNVSK"
     gene            4941..5768
                     /locus_tag="VF182_20940"
     CDS             4941..5768
                     /locus_tag="VF182_20940"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX6439596.1"
                     /translation="MIISENQRSSAGSPLDLNSIRQGLTAQRLGTEIHYFAEIGSTNT
                     YARRLAAQGARDGALIIADSQTDGRGRLGRHWVSPPNMNLYLSFVLRPKLPPARAPQI
                     TLMAAVALADALRSFLPVPPAIKWPNDITVNGKKLAGILTEMSCGDDTVDFVIVGIGI
                     NVNYPFDLMPEEIRQRATSILVERQRNVSREDLLWRLIKDLDRCYGELEENGFALLAS
                     RWEAYFGWRGRRVRIEILDQVLFGTAKGIDHDGALLLEDDHGKLHRVIAGDVTPVNP"
     gene            5772..6566
                     /locus_tag="VF182_20945"
     CDS             5772..6566
                     /locus_tag="VF182_20945"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX6439597.1"
                     /translation="MLLAIDIGNTNIVLGLYQGRKLITHWRLLTEAERTSDEYGVIIA
                     HLVDYEGFRCNQISAIVVSCVVPPMLTMVQELSKKFFKIEPLIVSPGIKTGMPILYEN
                     PRDVGADRIVNGIAAYERYKDSCIIVDFGTATTIDLISKKGEYVGGAIAPGLSISLEA
                     LSQRASKLPRIEIVKPREVIGRNTVNSIQAGIFYGYVGLVDGIVKRIQKEGGVQPKVV
                     ATGGLAPLVASECSTISDVDEFLTLQGLRIIHERNSIQEVKVRAQT"
     gene            6589..8688
                     /gene="fusA"
                     /locus_tag="VF182_20950"
     CDS             6589..8688
                     /gene="fusA"
                     /locus_tag="VF182_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX6439598.1"
                     /translation="MAAMELQNLRILGLLGQGGSGKTSLAEAMLFAGGATQRLGRVQD
                     GTSVLDHEPEEIKHQVSISAAFHSLSWKKHSLTLVDTPGYAAFLADSINCARAFGAAV
                     FVLNPSVGLRVESEQLWATANANEISRLFFVNKMDHEQADAHERIDPLLSRLDAKGVY
                     LQLPVGAGGEFKGVVDLPSMKAYLYEGSGGKFSESEIPADLREAAEDMRRRLLDSVAE
                     ADDQFLEKYLDGGEFSLEELKQTIREATRQRKIFPILYGSATHLIGIAQLLEAVLDYL
                     PAPGEETELEGNNPITREVETRRQDPGAPFSAYVFKTLIDPFAGKLSVMRVISGKITP
                     DETCYNANKQTKEKIGHMFRLEGKKQDAVKEAMAGEIVAAAKLKDVSSGDTLCDERAP
                     IQYPPPVHFSPVISFALQPKSKADEEKVPQGLHRMMEEDPTIDLHRDEETRDFILSGM
                     GQQHIEIIVEKLKRKYSAEVILKAPKVPYKETIRASANAQGKLKKQSGGRGQYGDTWL
                     KVEPLPHGKGFEFVDQIVGGAIPRNYIPAVEKGIKEAMTGGVVAGFPMVDVRVTLYDG
                     SYHDVDSSDMAFKIAASMGFKNAVEKAKPVILEPIMTMDVTVPEECMGDVIGDLNSRR
                     GKVLGMDSNGHTQVIKSRVPMSEVLKYAPDLRSLTSGRGEFHMEFSHYEELPAHLAER
                     VIKEAKTQAKTEQPSTH"
     gene            9010..10038
                     /locus_tag="VF182_20955"
     CDS             9010..10038
                     /locus_tag="VF182_20955"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6439599.1"
                     /translation="MSVIDSDAHVLETERTWDYMLESERSMRPRVVPTPSDPTSGGES
                     WLVDGTHIGKARNVGHDTAREAREMEDIRSRLAHMDELDIDVQVLYPTIFLRPFTRRP
                     EVELALARSYNRWLVDIWKAAPDRLRWVAVLPLLTMDKALEEARFAKENGACGIFMRG
                     LEGDKRISDGYFFPLYEEAGRLDLPICVHSATGSFAVHDYFIDECGFNKFKLAVVGSF
                     HSLIFNKVPERFPQTKWAFIEVSSQWIPYAIHDYARRFERGGTKVDKSDVLRRNRIWV
                     ACQTDDDLSYVLRYAGDSMLVIGTDYGHNDTSSEILALRKLREDGDVPPQVVKKILDD
                     NAKALYSL"
     gene            10069..11094
                     /locus_tag="VF182_20960"
     CDS             10069..11094
                     /locus_tag="VF182_20960"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6439600.1"
                     /translation="MVIDFQAHLFPEEYINELKRRDGAVILEEPDPYSGMTYFYDKKL
                     RCRINTAIFQGRDIERRLKHMDGLGIDIHVLTIPAPGADRFEGADAVAIARAANDAVA
                     AICRQHPTRFIGFFTLPTSSISASLDELERSVHQLGLRGFGCFSNLNGQALDREELFP
                     VYERLAKYKLPVYIHPTAPLASEATGMDIMPTLIFGWAFDSTVAMTRLVYGRVLERFP
                     EINFVVADVGGVLAFFAQRAKNIYSGRTEEIRQRYGLKENPLDSFRRFYVDTADHPAA
                     TLKSVLEFFGPERLVLGTNYPYGPEEGCVLIKNSLAAIDGLNLNKEDKEKILGGNAAE
                     ILGVGAA"
     gene            11091..12692
                     /gene="ggt"
                     /locus_tag="VF182_20965"
     CDS             11091..12692
                     /gene="ggt"
                     /locus_tag="VF182_20965"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX6439601.1"
                     /translation="MKFTKAARPVVMGQNGVVSAGHPLGALAGMKILQEGGNSVDAAL
                     AAAFVMSVVKPEASGPGGDLFALVYMKKAGKVMALNSSGPAPANATISYFQEQGLKSI
                     PINGPLSIAVPGAVDGWLELHKNYGTLELSRLVSDAVRIAREGFPISQDFAEAIEEHS
                     RESSWVDRFYRKPLGVSKPGKLLSQRGLARIFEKIADHGRGGFYSGEVADQIIRTVKA
                     EGGILAEEDLQKVIAQWMDPLKSSYRDTMVYQQPPVSQGFMVLEMLNIAEAWPFDSGS
                     MSRAEMIHLQVAAKKLAFEDRIRYLEDPSFGDPKIGMLLSKDYAAALREHADDALKRS
                     APAAPNQSSDTTYLCATDRDGNAISLIQSVFAPFGSGVIAGETGVVMNNRLCSFGLDP
                     AKANSLMPGKRPAHTLNTYMVFKGDEVFAVGGSPGADEQPQTNFQIIHDLVDLKMDPQ
                     SAVEAPRWSHQPGTPPRDQLPEMLRMEEGFDAQTLEDLRNRGHKLSVVDRWSFGSAKI
                     IVRDHENGCWMGGADPRRLAYALGW"
     gene            12864..13904
                     /locus_tag="VF182_20970"
     CDS             12864..13904
                     /locus_tag="VF182_20970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6439602.1"
                     /translation="MFTEIQCPVGSSPEKRLDEFLEQAELADDLDFTTFWIAEIHCQP
                     RFSLLSAPYVVLGAVAQRTKRLRLGVAVNTLPIHHPVQLAEEAAMLDILSGGRMDFAA
                     GGGHPHSRAYECFGADHKITHELMVEALEIIRRAWSEETLNFTGRYFQIPEVIVNPKP
                     VQRPLPSFYMATSSLDGVAVGARLGVNLLLPIHTRTPEQVIEFSEAYWQGLKTNGHDP
                     KSKELGLLVPMHLAPSIAQARSRSQDGIMSYFKTIADMRNDYVDWLTRRGDELPVRLR
                     TTSGASVSFKDICDRHAIVGNSESALHRLKALTAATGATNLLTWFNIGNVPHALVQES
                     MQQFAAEVMPHL"
     gene            13925..14278
                     /locus_tag="VF182_20975"
     CDS             13925..14278
                     /locus_tag="VF182_20975"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006634040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX6439603.1"
                     /translation="MENLTEQKCEACRVGAPPVTADEIQELHPRVSEWLIITEENIPK
                     LDRQFKFKNFREALAFTDAVGAAAEEEGHHPRITTEWGKVGVTWWTHKIRNLHKNDFI
                     MAAKTDAIYKNYPQS"
     gene            complement(14658..16271)
                     /locus_tag="VF182_20980"
     CDS             complement(14658..16271)
                     /locus_tag="VF182_20980"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HEX6439604.1"
                     /translation="MAFYIIFNLLIFLSGQLQAEPAPDLEEQTRAVATELRCVVCQNL
                     SVADSPSEMAQQMRASVREQLQAGKSPQEIKDFFVSKYGEWVLLTPTNTGFNRLLWWL
                     PLTVLIIGCIVALLLARRWAARKAHRVAPKVDPSVAAQLRKEMKAKNVEVPDSDDSTA
                     RAQLVRESIRLREELAELEFDFQSGKLSQADYNGLRSEVDAKQTLVLQQLSYLPPEPP
                     AKAPVEDKPARRQEAPSRIRFRRWQLATGGALLLLFGLALGLLLTQSLRPRSSEQDSM
                     TGDFLTGTTPDNAATRSALEEGKRAFAKQDFPKAIEAFKKVLEADPNQPEAHSYMGMI
                     LIQAGHADGAMMAFDRALAQAPNYPMALWGKGMILYREKKDYDGARVVLQQLLQLIPP
                     GEDRAEIEKIVASFPPPGSNSGHGVAAPSPDSRQTVSGKITLDPKVKVTNSKAILFII
                     ARPAGPGGGPPLAVKKIDQPVFPLSYVIGAENVMMQGTPFSGKINLSVRLDNDGNPTT
                     RTAGDLMGNYQEPVEVGAKNIDIVIDQAL"
     gene            complement(16276..16842)
                     /locus_tag="VF182_20985"
     CDS             complement(16276..16842)
                     /locus_tag="VF182_20985"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX6439605.1"
                     /translation="MRWRRVALTLLIIAPILALLAYGFSRDARYITSPLLAKPATPFS
                     IELFKGGKLRLEDLRGKAIFLNFWASWCPPCRAEARDLEAAWQRLKDKNVIFLGVALQ
                     DTEQASLEFLKEFEVTYPNGKDVSGKIAIDYGVWGIPESFFIDPQGRITYKHVGGIHA
                     AMVTEKLLEAQQGLVSANEGRGEFQSVR"
     gene            complement(17065..19026)
                     /locus_tag="VF182_20990"
     CDS             complement(17065..19026)
                     /locus_tag="VF182_20990"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX6439606.1"
                     /translation="MSATIGYTSILIALFAAAAGIVAPVVSMRCGHARYSSAIEKFIS
                     TQFVFVTVAASALIYALVSADFSVRYVAFNTTLATPFYYRVTGLWGALEGSLLLWEWI
                     LIIFSGLVAWIYRGRHKETLPWVLMVFSIVSFFFLAVIAFVSNPFETIFPVPPDGRGL
                     NPLLEDANMMTHPPLLYTGFVGLTVPFAFAIAALIVGKLDEAWITTTRRWTIIAWFFL
                     TAGNLVGAWWSYHVLGWGGYWAWDPVENAAFMPWLPATAFLHSIQVQERRRMLKVWNL
                     SLIIIAFSLTIFGTFLTRSGILSSIHAFSSGPVGIFFLGFLTFVLLGSFGLLAYRSDM
                     LKEQPELDSMVSRESAFLLNNIVLVSALFTIFLGTIFPLLSEAVAGVQVSVGAPYFNS
                     VTVPLFLFMVFLMGVGPIIAWRRASWDNLKRNFLRPAIGALIFALFLFAWKVKDFLPL
                     LGFTLLAFVVMTIVYDTALALRARHRLAKEGILRGLITLTRRNQRRYGGLVVHLGVVL
                     IIMGIAGSMTYSTEREATLGLNESLSIGNYQIRFEGLRGSQQPTHYRVEGAFRVLRQD
                     SDIGLLSPALKFFPSQQSPIGRAVHRSSPSEDIYLILSGFSDLDKNQATLKVLVRPLV
                     VWMWIGGLVIALGTLITIWPMCKPVPTET"
     gene            complement(19023..19466)
                     /gene="ccmE"
                     /locus_tag="VF182_20995"
     CDS             complement(19023..19466)
                     /gene="ccmE"
                     /locus_tag="VF182_20995"
                     /inference="COORDINATES: protein motif:HMM:NF009727.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX6439607.1"
                     /translation="MAKNKRFLIGGLIILAALSYVIYGGMQQALVYFVTPSELKANET
                     SSANKFLRMGGMVVNGSLQKDVKNLTYHFELTDGASTFPVFFRGVPPDLFREGTGAVV
                     EGRIGKDGVFQASTIMAKHAEEYSPHEDRQGAPHSFVPAKEYTSK"
     gene            complement(19608..20315)
                     /gene="ccsA"
                     /locus_tag="VF182_21000"
     CDS             complement(19608..20315)
                     /gene="ccsA"
                     /locus_tag="VF182_21000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX6439608.1"
                     /translation="MKREQSQKRGAGFLGALALIMIALGLYWGLVQAPPDALQGDVQR
                     IMYLHLPSILTAYLSFFLVFIGSCLYLWKREKRDDNLAHAAAELGVLFTALTIIEGSI
                     WGKPTWGVWWTWDARLTLTAILLLIYSGYLMLRSLIDDEDRAALSAAILGVIGFLDIP
                     LIHMSVYWWRTLHQPPSILRPDKLPWENVHPAMLTALAINFVGFLLLYFYLLSLRLRL
                     GEVRSEIKARRLSQGAL"
     gene            complement(20315..20992)
                     /locus_tag="VF182_21005"
     CDS             complement(20315..20992)
                     /locus_tag="VF182_21005"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX6439609.1"
                     /translation="MKLLRETYLVLRKDLVLQLRRRDSLLTMFFFGTLLLFVFHFSFD
                     LTPDRIAELAPGLLWLAFLFTGTLGLAQLFGAERENQCLDALLLSPLDRGALFLAKVC
                     LNFILMLVVELVIIPLFWILFNLQTWTLMPQVFLIALSGTVGFCVLGTVMSALTLRAR
                     ARELLLPLVLFPLMIPVILATIQCLAAVFRAGEIGDALPWLRLLIGFDVIFLTLGVLV
                     FDWVIEV"
     gene            complement(20989..21696)
                     /gene="ccmA"
                     /locus_tag="VF182_21010"
     CDS             complement(20989..21696)
                     /gene="ccmA"
                     /locus_tag="VF182_21010"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEX6439610.1"
                     /translation="MHLTVQNLSKAYGFFWALKDLNLDLPPGELVALLGPNGAGKTTL
                     LRLIAGLTPPSLGTIELDSLPSSRAYGSARAQIGLLTPAAHLYEDLTVRENLEFFTSL
                     YGQNHDAVSIDAALSKVELGSRADELASTLSSGMKCRLSIAKWNLLKPGLLLLDEPYG
                     VLDGSGVDLLEEFLKAHCRKGNLVILASHHVSRVMKICTRAIILHRGRMTFNAPRQQP
                     WPDFDKAFQEFSPHRDS"
     gene            21876..22571
                     /locus_tag="VF182_21015"
     CDS             21876..22571
                     /locus_tag="VF182_21015"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439611.1"
                     /translation="MNPVVTVTGAWNAFATKIAAFLPDLIAAIFIVVFGWILCNIIKR
                     IVIRVLRLVHFDTVAERAGIKQILERGGIQQNPSEILGLLFFWFLFLIVIVATLETLN
                     LSGATDTLNAIYLYLPKIAAAIVMLILGLYFANFLETVTRTSCANAGLEQAGSIGRAA
                     YVITTIFVVAGIFEILDIASEIVIWAFILVFGAVCLALALAFGLGGRDVAGRYLEKWL
                     EQKNNDETKLMHP"
     gene            22774..24180
                     /gene="icd"
                     /locus_tag="VF182_21020"
     CDS             22774..24180
                     /gene="icd"
                     /locus_tag="VF182_21020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020563805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX6439612.1"
                     /translation="MAEYKVVEVPKDGAKITMGANKQLQVPDNPIIPFIEGDGTGRDI
                     WRASVRVFDAAVAKAYKGKKKIRWMEVYAGEKSFKQYNSWLHEETVPAFSEFLVGIKG
                     PLTTPIGGGMTSLNVGLRKLLDLYVCQRPVRYFNGVPSPLKHPEYVDMVVFRENTEDI
                     YTGIEFPNGTEPNAKFKSLLKETFPKEFAKIRFPNTAAIGLKPVSVEGTERLVRAAIQ
                     WALKNKRKNINFVHKGNIMKYTEGAFAEWGYALAKREFRNDVVTERESWILGNKEADA
                     NVSAEANAKMIDPGFDMMSPAQQNDIKKEVEEALKLWPTHGDGKWKKKLMLKDSIADV
                     TLQFVLIRPKDYDVIATMNLNGDYLSDALAAQVGGIGIAPGANINYDSGHAVFEATHG
                     TAPKYADLDKVNPGSVILSGEMMLRYMGWTEAADLIIKGIEGAVNAKTVTYDFERLMP
                     GSQLVSCSGFGDAIIANM"
     gene            24234..25175
                     /gene="mdh"
                     /locus_tag="VF182_21025"
     CDS             24234..25175
                     /gene="mdh"
                     /locus_tag="VF182_21025"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX6439613.1"
                     /translation="MTRNKIALIGAGNIGGSMAHLCLLKGLGDVVLFDVVEGLPQGKA
                     LDLSHAGPVEGFDGNVIGTNSYEDIAGANVCIVTAGIARKPGMSRDDLLGTNAKILTS
                     VADGIKKHAPNSLVIVVTNPLDAMVTLMKRATGFPKNRVVGQSGILDSSRYRSFIARE
                     LNVSVNSVAAMVLGGHGDDMVPVRSTCIVGGVPVEKLIPSKRLDEIEARVRNAGGEVV
                     ALLKTGSAYFSPASAAIRMTEAYLFDKKEILPCAALLEGEYGVNGYYFCVPVIIGAGG
                     VEKIVEIDLHAADRRAFDESLNHVKDIVQAMDRVVGA"
     gene            25258..26421
                     /gene="sucC"
                     /locus_tag="VF182_21030"
     CDS             25258..26421
                     /gene="sucC"
                     /locus_tag="VF182_21030"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX6439614.1"
                     /translation="MNIHEYQAKEILKGYGVPVPRGEVAATPEEAKAAAIRLGGGTCV
                     VKAQIHAGGRGKGGGVKLARNPEEAEQIAREMIGKNLVTHQTGPEGRQVRRVLIEQGL
                     NIDRELYLALVLDRAQSRVSMICSSEGGVEIEEVAEKNPEKILKETIDPIIGLAGFQC
                     RRLAYGLNIPGELIGKMTAVMQALYRAFDDADCSIAEINPLIITKEGQVMALDAKMNF
                     DSNALYRHKDIVALRDLNEEDPREIEASKFDLSYISLDGNIACMVNGAGLAMATMDII
                     KLCGGEPANFLDVGGGASKEKVAQAFKILLADPRVRGVLINIFGGIMRCDVLAQGIVD
                     AAHEMNIQVPLVVRMEGTNVAQGKKILADSGLAIIPAETMAQAAEKIVAAVEK"
     gene            26595..27473
                     /gene="sucD"
                     /locus_tag="VF182_21035"
     CDS             26595..27473
                     /gene="sucD"
                     /locus_tag="VF182_21035"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015891769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX6439615.1"
                     /translation="MAILVNKHSRVLTQGITGATGQFHTKACKEYGTQMVGGVTPGKG
                     GTNFEGIPIFDTVTEAVAETAATATVIYVPPPFAADAILEAADADIPLIICITEGVPV
                     RDMVRVKRYLSGKTSQLLGPNCPGVITPGECKIGIMPGHIHRQGSIGVVSRSGTLTYE
                     AVDQLTRLGLGQSTCVGIGGDPVHGFEFVDVLDLFNRDPGTQAVVMIGEIGGSAEEDA
                     AAWIKANMKKPVIGFIAGQTAPPGKRMGHAGAIISGGKGTAGEKIEAMKAAGIRMSDS
                     PATIGQTMQEALRSHE"
     gene            27466..27894
                     /gene="ndk"
                     /locus_tag="VF182_21040"
     CDS             27466..27894
                     /gene="ndk"
                     /locus_tag="VF182_21040"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006341801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX6439616.1"
                     /translation="MSEVTLSIIKPDAVQRNLIGEILRRFESAGLKVVAGKLLRLSSE
                     QAQAFYAVHKERPFFAGLCAYMSSGPILVSVLEGKNAIAKNREIMGATDPAKAAPGTI
                     RKDCGTDVEANAVHGSDGPETAATEIAFFFKPEEVFRSSK"
     gene            27923..28981
                     /gene="rlmN"
                     /locus_tag="VF182_21045"
     CDS             27923..28981
                     /gene="rlmN"
                     /locus_tag="VF182_21045"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX6439617.1"
                     /translation="MKPNIKDLSFNEFEAYLFERKHPAYRARQVWQWLYQKRAASFAD
                     MTNLSGVFREQLAADFSISRLAVERRADSSDGTVKFLFRLADDRNIESVLIPEAKRLT
                     LCISTQVGCGFGCAFCATALLGLKRNLRASEIVDQILEASRTLTEDRRITNVVLMGMG
                     EPLANYAQTIKALAVMTDNSWGIGISPRRITLSTVGLVPQIRKLMDETKVSLAISLHA
                     ATDELRSRLMPINRKYSLRQLMECCRALPIPRRKRITFEYVLLHGVNDSAADAYNLVE
                     VLRGVRCKINLIPFNAHQGSAYERPETKNVEAFQQILSCQGLQVNVRRPRGDDIAAAC
                     GQLQGETIKEQESAAGHC"
     gene            28996..29856
                     /gene="mtnP"
                     /locus_tag="VF182_21050"
     CDS             28996..29856
                     /gene="mtnP"
                     /locus_tag="VF182_21050"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX6439618.1"
                     /translation="MSKQVIGVIGGSGLYQIEGLQRVKEVDLNTPFGKPSDKFIRGWL
                     GDTELLFLPRHGRGHRWLPTEINFRANIFGMKKLGVERIISVSAVGSLQEKIAPGHVV
                     IPDQFIDRTTQRPSTFFGKGIVAHVSLADPFCKELSATLAKAAMVAGATIHQRGTYLC
                     MEGPQFSTRAESHLYRNWGADIIGMTNLQEAKLAREAEICFATLALATDYDCWNESAG
                     DVEIEHVLAVLQQNVDLAKKTIRQVIKNLSAARSCGCALALQTAIITEKSQIPKKVRR
                     DLRPIIGKYL"
     gene            29853..30758
                     /locus_tag="VF182_21055"
     CDS             29853..30758
                     /locus_tag="VF182_21055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX6439619.1"
                     /translation="MSILVVGTVAFDSIETPLGTAERILGGSASYFALGASFFAPVRI
                     VGVIGQDFPQEYLDLFRQRQIDLEGIKKASGDTFYWRGRYHEDINVRDTLELHLNVLS
                     GFVPNLPEQYRDADYVFLGNIDPLMQLEVLNQIRRMRLVVCDTMDHWIRESLDGLRQV
                     LKRIEMLVINDSEARLLSGQSNIVRAARDILKMGPQVVLVKRGEYGVLQFSNSSIFAT
                     PAYPLEEVFDPTGAGDSFAGGFLGQLARSGELTQQGIRRAIVYGSVVASFTVEDFGVK
                     RLAKITLQDIEERYRSFAQLTDFHT"
     gene            30763..31716
                     /locus_tag="VF182_21060"
     CDS             30763..31716
                     /locus_tag="VF182_21060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6439620.1"
                     /translation="MDLSIVIPVFNEEENVEPLISEIEAALNSLSKHYEIVVIDDGSR
                     DNTFQILAQIYRTDPHLRVVKLKRNFGQTAALAAGLAHARGEVVVMLDGDGQNDPADI
                     PALLVKLEQGNDIVCGWRYRRRDPFLTRRLPSMIANSLISWTTRVKLHDYGCTLKAMR
                     KEIAKNLKLYGEMHRFIPAIAYERGAKIAEVQVNHRPRLRGKSKYGITRTVRVILDLI
                     TVKFLMSYSTRPSHIFGILGIGSGALGFILAIYLTVQKLMYNESIGTRPLLLLAVMLI
                     FVGFQFVTMGLLGEMLSRTYHESQDKAVFVVGEILGERSCV"
     gene            31713..>32184
                     /locus_tag="VF182_21065"
     CDS             31713..>32184
                     /locus_tag="VF182_21065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEX6439621.1"
                     /translation="MKLAVIGTGYVGLVAGTCFSETGNEVVCVDIDEAKIVMLQQGKI
                     PIYEPGLEEMVRRNAEEQRLIFTTDVKSAVQNSSIIFIAVGTPQGLNGHANLEYVRNT
                     AKSIAQAMDGFKIIVTKSTVPVGTADQIRQWVSEETSQPFTVLSNPEFMKEGAAV"
BASE COUNT         7712 a         8329 c         9056 g         7087 t
ORIGIN      
        1 ccagatctat aaccgcttca aagattacac gatgctactg ccggattggt acgtagcgaa
       61 cctcgaactc tgcgaaaatt tcggccatat cgccggaaac atcgtggagt gcggagtatg
      121 gaaaggcggg atgagcgctg gaattgccta tttgcttggt gaccagcgcg cctattatct
      181 ctacgatagt tttgaaggac tcccagccgc tggggatgaa gacgctatac gcggtatcgt
      241 ggcttcggcc aagagttggg aagggaaatt gaaggcgtct caggagcacg cgaatgaggc
      301 gatgaagctg tctggcgcaa aacgcgttac gattatcaag ggatggtttg gtgaaacctt
      361 gtccacttat gacgctgggc cgattgcaat tcttcggttg gacagtgatt tctattcgag
      421 tgtcatggac tctttaatca acctttggcc tcatgtggtc actggtgggc tcataatcct
      481 tgacgactac tacacctgga gcgggtgcac ccgcgcggtc catgattttc tttctcacaa
      541 tgagctaaac gagccgattc agcaattcca cgagacgtac gcgtatgtag ttaaggaagc
      601 ggatacgaac cagtcctccc ggcgttgtaa taaagtggag gcttcggttg gagtgcagcc
      661 tcactaaaac cctgttttca tgatgggcta atccgactat cccgccgaac ctttgtggcg
      721 gacagaattt aacgtatggc cgcgattctc gcgtgctttc cgactctggc tcggttggat
      781 tcttgctttg tgccacggac aaacgaatga gtaagattct agttgttgag ccctacaaaa
      841 tcctacagcg agccattgct gtgagtcttt ttccagagca tgaagttgag gtgactgagc
      901 aggttccaga gatgagcgcg cttgagcagc gctcttatga cgcagccatc atcgatgccg
      961 gggccctgcg cgaaaaaaat accgccggcg atctgaaccg ctgtttgcag gactggccaa
     1021 tacccataat ttggcttgag gagaacattt caaaggccgc gccggtgcgc gatcaactgg
     1081 taattgtgcg gaagccgatc gcgcgcaacg atctcctcgc agcgctggct cgctgtcagg
     1141 cgaacggatc gaccattccg ctgaacgagg ccggtgaaaa gcttcggaat gaacccgttt
     1201 tggctggcac tgaacccagg ccagattctg agcacagggc gcggatcata gaactggtcg
     1261 atgtcgtcga agaagaacca accgccggta agcaagataa gcggctggag agaaagaaac
     1321 cataggccat gcaactggac aaagcttatt cgcataaatt aatcgaggcg aagtggtacc
     1381 aacattggac cgaattggga gcgttcaagc cctcgaaagg ggacagtcct tacttttcaa
     1441 tggtgattcc gccccccaat gtgaccggat cactgcacat ggggcacgct ctcaacaaca
     1501 cacttcaaga catactttgc agatacaaac ggatggacgg gtttcgggtg ttatggattc
     1561 cgggcacgga tcatgccggc attgcgaccc agaatgttgt cgagcgtagc attgcggctg
     1621 agggtctgac ccgcaatcag ctcggacgca aaaaatttat cgagagggtt tggtcatgga
     1681 agcagcaggc gggtaatgcc atcttgcatc agctaagagc gcttggagtt tcttgcgact
     1741 gggatcatga acgattcacc atggatgaag gcctctcgcg cgccgtgcgt gaagctttcg
     1801 tgcggctgtg ggatgataag ctgctctacc gggcgcaaag actgatcaac tggtgtcctc
     1861 gatgcaaaac cgcgctctcc gacattgagg ttatccatca ggaatccgat ggcaatctct
     1921 ggcatatcgg ctatccattg gcggaggatt cctcccggga gctcgtcgtg gcgaccacgc
     1981 ggccggaaac catgctcggt gataccgcgg tagcggttca tcccgaagac gagcgctatc
     2041 aaaatctcat cggcaaaaat atccggctgc cgctgagcgg acgcatcgtt cccctgatcg
     2101 ctgatccatt tgtcgatcgt gaattcggaa cgggagcgtt gaaggtcacg ccgggtcatg
     2161 acttcaacga ttttgaaatt ggcgagcgat tcgctcttga caagatcagt attttcgatg
     2221 ctgagggccg catcgatgcc ggggcgtttc tgcaacgtgg cgaaaccggc gaatggatcg
     2281 accggtatcg tggtaaggat cgcttcgaag cgcgccggct ggtagttgcg gatttgaagg
     2341 aacggagatt tcttcggaaa atcgagcctc acaaactttc ggtgggccat tgctatcgct
     2401 gccaaagcgt cgtcgagcct tatctcacac cgcaatggtt cgtgaatatc acacctttag
     2461 cggcccccgc gatccaagcg gtgcgccgcg gcgatatccg gattattcct gaaggatgga
     2521 gcaattctta ctttgcctgg atggaaaaca tcaaggactg gtgtgtctcg cgacaaatct
     2581 ggtggggaca tcaaattccc gcgtggtact gccgctcatg cgatggtgaa aacatcgtcg
     2641 agggtcatga tggcgagtac agcctgacca aagaagctca acccatcgtc tcccgcgctc
     2701 cgccgaagac ctgccctcgc tgcggtgcgg atgatttggt ccaggacccg gatgttctcg
     2761 acacttggtt ttcttcgagt ttatggccgt tttccacctt gggatggcct gaggacactg
     2821 atgacctgaa aagtttttat ccaacctcta cattggtgac cgcgttcgat atcctatttt
     2881 tctgggtcgc gcgtatgatc atgatggggc tcaagtttat gggcgatgtg ccgttctatg
     2941 atgtttacat ccacgccctg gtgcgggatg aggaaggtca gaaaatgtcg aagtccaaag
     3001 gaaacgtcat cgaccctttg gacattatgg ctgaatatgg caccgatgcg tttcgcttca
     3061 ccttggccgc catggccgca atgggacgag acatcaaact tgctgaagaa cgaatctccg
     3121 gctaccagaa tttcgtcaat aaactgtgga acgcttcccg attcgttctg atgaatttgg
     3181 cgcaaagcgc tgccgccaaa caggctgatg aaccgtctgc tctcgacagt accgagctcg
     3241 gcttggcgga tcggtggatt ctttcgcgtc tggcttcaac agttcgcgac gcgcgcgtcg
     3301 ctatcgacgg ttatcgcttc aacgattacg ccaatattct ctatcagttt acctggcacg
     3361 agttctgtga ctggtatatc gagatgagca agctgtcgct taatggacaa ttcgccgatg
     3421 atcccgaacg aagccgacgt atgctggtcg aagtcctaga gcagattctt ttgttattgc
     3481 acccgatcat gccctttatt accgaggaaa tttggcaagg gctgggcgac ggcagggaaa
     3541 caataatgct gcaaaaatat ccgcgagcgg aagctgactg gatcgatgcc aaaactgaaa
     3601 agcaaatgga tcatctgatg ggcgtggttc gagcgatcag aaatctgcgc actgagctga
     3661 attgcccgcc ggctaaggaa atcaaggtta tcttccacgg ttctgacggc gatcttgcat
     3721 ttctcgactc gcacatgcct tatcttcgcg cattggctcg agtgggttcc gctgatttta
     3781 ctccatcggg agatcggccc aagggagcgg ccaccgcgat ggttggcgcc accgaaattt
     3841 atcttcccat cgaggatctc ctcgatctgg acgaggaaca cgcacgattg accaaggaga
     3901 tcgctaagat tagcgcggaa atagctcgta ctcagaaaaa gctcggcaat ccggagttcg
     3961 tcaataaggc caaagcagag atcattcaaa aagagcgtga aaaggcaatc caacacgcag
     4021 aaaagctgcg cacgcttgag gcgagcctag atcagatcca ggagattcaa gcaggaagga
     4081 actaggcaaa tggacattct tgtcagtccg cagatcgagc gcttaatccg ggattctttg
     4141 gacgaagata tcggcgccgg cgaccttgcg acgatggcga caatctcggc agaatctcaa
     4201 ggcacagggc tttttcgcgc caagaaacaa ggcgtcgtcg caggtttggt gttgttggag
     4261 cgaattttct actttatcga tcccaaggta agcgtccgtc ttctcacgaa ggatggcacc
     4321 caggtcgcag aaggcaccgt cgtggccgaa gccgaagggc cggttcgcgc gctgcttttg
     4381 gctgaacgga cagcgttaaa ttttcttcaa cgactatccg ggaccgcgac tctaacgcgc
     4441 cagtatgtcg aggcggtaaa agattttccg tgtaaggtgc tcgataccag aaaaacgacc
     4501 cctggacttc gtacgctgga gaagtatgcg gttcgaatgg ggggaggcac gaatcaccgg
     4561 atcggcctct acgatgcggc tctggtaaag gacaaccata tcgaagccac cggatcgatc
     4621 gccgaggcag tcaaagcggt cagactgcat gcgccgttca tggctaaggt tgaagtcgaa
     4681 gcgggtaact taacccaggt tcaggaagca atagacgccg gcgccgatgt gatcatgctc
     4741 gacaatatgt cggttgcgca aatggccgaa gcggtgcgtt tggtgaacaa gcgcgcctgg
     4801 gtggaagcgt ccgggggcat cacccttaat actatccgtc agatcgccgg cactggggta
     4861 gattttatct cttcaggcgc gttgacacat tcggctccgg tggtcgactt taatatgaaa
     4921 ataaccatga acgttagtaa gtgatcattt cggaaaatca acgaagcagc gccggttcgc
     4981 ctctcgatct taattcgatc cgccaaggat taaccgccca acggctcgga actgagattc
     5041 actactttgc tgagatcggc tcaaccaaca cctacgctcg ccggctggcc gcccagggcg
     5101 ccagagacgg cgccttaatt atcgcggatt cgcaaacgga cggccgaggt cgcctcggca
     5161 ggcattgggt gtcgccgccc aatatgaatc tctatctttc tttcgttttg cgacccaaac
     5221 tgccccctgc cagagcgccg caaatcacct tgatggcggc cgttgcttta gccgatgcgc
     5281 tgcggtcctt tcttccagtt cctccagcta tcaagtggcc caatgacatt acagtgaacg
     5341 gtaaaaagtt ggcgggtatc ttgactgaaa tgagctgcgg cgacgacact gtcgacttcg
     5401 tgatagtcgg cattggtatc aatgttaatt atcctttcga tctgatgccc gaagaaatcc
     5461 gccaacgcgc gacgtcgatt ttggtcgaac gccagcgcaa tgtaagccga gaagatttgt
     5521 tgtggagatt gattaaagac ctggaccggt gctatggaga actcgaggaa aatggtttcg
     5581 cgctgctggc atctcgttgg gaggcttatt ttggatggcg agggcggcgg gtgcgcattg
     5641 agattttgga ccaggttttg ttcggaacag ccaaaggcat cgatcatgac ggcgcgcttt
     5701 tgctcgaaga cgaccacggc aagctgcaca gagtcatcgc cggggacgtc actccggtga
     5761 acccataaat catgctgctc gccatcgata tcggcaatac caacatcgtt ctcggactgt
     5821 accagggtag gaaactaatt acccactggc gactgcttac cgaagccgag cgcacctccg
     5881 acgaatacgg cgtcattatc gcccatttag tcgattacga aggatttcgc tgcaaccaga
     5941 ttagcgccat cgtcgtatcc tgcgtggtgc cgccgatgct gacgatggtt caggagctga
     6001 gcaagaagtt ttttaaaatc gaaccattga tcgtcagccc cggtatcaaa accggcatgc
     6061 cgattcttta tgaaaatccg agggacgtgg gcgccgaccg gattgtcaac ggcatagccg
     6121 cctacgagag atataaggac tcctgcatta tcgtcgattt cgggactgcc acgaccattg
     6181 atctgatttc gaagaaaggg gaatatgtcg gcggtgcgat cgcgccgggt ttgtcgattt
     6241 ctttggaagc tctgtcgcaa cgcgcctcca aactgcccag aatagaaatc gtcaaaccac
     6301 gggaagtgat tggccgcaat acggtgaact cgattcaggc aggaatattt tatggctatg
     6361 tcggcttggt cgatggtatc gttaagcgca ttcagaagga aggcggagtt cagccaaagg
     6421 ttgtcgcgac cggcgggctg gcgccactgg tggcttcgga atgctctacg atcagcgatg
     6481 tcgacgagtt tttaacgctt cagggactgc ggatcattca cgaacggaat tctattcaag
     6541 aggtgaaggt aagagcgcaa acatagcaaa aaggaggaac ccacagttat ggctgcaatg
     6601 gagctgcaaa accttcgaat cctcggcctt ctgggtcaag gaggctcggg aaaaacctct
     6661 ttagcggagg cgatgctgtt tgccggcggg gcaactcaac gactcggacg ggttcaagat
     6721 ggcacgtcgg tgcttgatca tgagcccgaa gaaatcaagc accaggtttc tatctcagcc
     6781 gcttttcact ctctttcatg gaaaaagcat tctttgactc tggtggatac cccgggctat
     6841 gcggcgttcc tcgccgattc aatcaactgt gcgcgtgctt tcggcgccgc agtatttgtt
     6901 ctcaatcctt cggtgggatt gcgggtcgaa tctgagcagc tatgggcgac ggctaacgcc
     6961 aatgaaattt cccgtctttt tttcgtcaat aaaatggatc acgagcaggc agatgcgcac
     7021 gagcgaatcg atcctttgct ttccaggtta gatgccaaag gcgtttatct gcagttgccg
     7081 gtgggagccg gaggcgagtt caagggcgtg gtcgatctgc cctcaatgaa ggcctatctc
     7141 tatgaaggaa gcggcgggaa atttagcgaa agcgaaatcc ctgctgatct ccgggaagcg
     7201 gccgaagata tgcgccgacg gttattggat agtgtcgccg aagcggacga ccagtttctc
     7261 gaaaagtacc tcgacggcgg cgagttttcg ctggaggagt tgaagcaaac cattcgtgaa
     7321 gccacgcggc aacggaaaat ctttccgatc ctctatggct ctgcgacaca tctgattgga
     7381 atcgcgcagc tgctcgaggc ggtgcttgat tacttacctg cgcccgggga agagacggag
     7441 ttggaaggga acaatccgat aacgcgtgag gtcgagacaa ggcgccagga tcccggcgca
     7501 ccgttttcgg cgtacgtgtt caagactttg atcgatcctt ttgccgggaa gctgtcggtg
     7561 atgcgggtga tttccggaaa aattacgccg gacgagacat gttataacgc caacaagcag
     7621 accaaagaaa agatcggcca catgttccgg ttggagggaa aaaagcagga cgccgtcaaa
     7681 gaagcgatgg ccggcgaaat cgtcgccgcg gcgaagctga aggatgtcag cagcggcgat
     7741 accctgtgcg atgaacgggc gccgattcag tatccgccgc ccgttcattt ctcaccggtg
     7801 atttcgtttg ccctacagcc caagagcaaa gcagacgaag aaaaggtgcc gcagggcctg
     7861 caccgaatga tggaagaaga tccaaccatc gatcttcacc gcgatgagga aacccgcgac
     7921 tttattctgt cgggcatggg ccagcagcat atcgaaatca tcgtcgaaaa gctcaaacgg
     7981 aagtacagtg ccgaggtcat tttaaaagcg cccaaagtac cgtacaaaga aaccatccgc
     8041 gctagcgcca acgcgcaagg aaagttaaag aaacaatccg gtggccgcgg tcagtatggc
     8101 gacacatggc tcaaagtcga accgctgccg cacggcaagg gatttgagtt tgtcgatcag
     8161 atcgttggcg gcgcgattcc ccggaattac attccggcgg tggagaaagg catcaaagaa
     8221 gctatgacgg gaggcgtggt agcggggttt ccgatggtcg acgtgcgtgt gacactttac
     8281 gacggttcct accatgatgt cgattcatcc gatatggcat ttaagatcgc cgcttccatg
     8341 ggattcaaaa acgccgtgga aaaagccaaa ccggtgattt tggaaccgat catgaccatg
     8401 gatgtgacgg tgcccgagga atgtatgggc gacgtcatcg gcgatttgaa cagtcgcagg
     8461 ggcaaagtac tgggcatgga cagcaatggg cacactcaag tcatcaaaag ccgagtgccg
     8521 atgtccgagg tgctgaagta tgcgccggat ctgcggtcgc ttaccagcgg tcgcggcgag
     8581 tttcacatgg aattttcaca ttatgaggaa ttgccggctc atctggctga aagagtgatc
     8641 aaagaagcca aaacgcaagc caaaacggaa cagccgagca cgcactgacg atttagacga
     8701 actatattga tgtgttgcga gggtcatcgg ccgagcagga acgaccgctc accgttggca
     8761 gctagtcagt taaagatctt gagatcgagg cagtaatgga acaatcattt gatcaattca
     8821 acgcagcgtt gctgtattgc aataagtgcg gcagggcgat gccggtcaga cagcgtttgc
     8881 tgctggtact gcccgatgga gaactctatg attatacgtg ccagacgtgc catagctcag
     8941 tgggatcaaa aaccgaacgt gctggcatcg atcccacgac acgaaagact aggataggag
     9001 gctgatcgaa tgtcagtgat cgattctgac gcgcacgttt tggagaccga gcgcacatgg
     9061 gattatatgc tggaatccga gcgctcgatg agaccgcggg tggtgcccac gcctagcgac
     9121 ccgacctcgg gaggtgaatc ctggttggtc gacggcactc acatcggcaa ggcgcgcaac
     9181 gtcggtcacg atactgcgag agaagcgcgg gagatggagg acatcagatc gcggctggcg
     9241 cacatggatg agctcgatat cgacgtccag gtgctctatc cgacgatatt tctccgtccg
     9301 ttcactcgcc ggccggaggt cgagttggcc ctcgcgcgca gctacaaccg atggctcgtc
     9361 gatatttgga aagcagcgcc cgatcgcctg cgctgggttg cggtgttgcc gctcttgact
     9421 atggataagg cgctggaaga agcccgcttt gccaaagaaa acggagcgtg tggcatcttc
     9481 atgcgcggtc tggagggtga taagcggatc agcgatggct atttcttccc gctttatgag
     9541 gaggccgggc gactcgattt accgatttgc gtccactccg cgacgggaag tttcgcggtg
     9601 cacgactatt tcatcgatga atgcggcttt aataaattca agctggctgt agtcggatcg
     9661 tttcattcgc tcatcttcaa caaggttccg gaaagattcc cgcaaaccaa atgggcgttt
     9721 atcgaggtca gctcgcaatg gatcccctat gcgattcacg actatgcgcg acgcttcgag
     9781 cgcggcggga ctaaggttga caaatccgat gtgctgcggc gaaaccgaat ttgggtcgcg
     9841 tgccagaccg atgatgatct ttcctatgtg cttagatatg ccggcgatag catgctggtg
     9901 attggcacgg attacggaca taacgatacc tcatcggaga ttctggcgct gcgcaagctc
     9961 agagaagatg gcgacgtgcc gccgcaggtg gtgaaaaaaa ttctcgacga caacgccaag
    10021 gcgctttata gcctttaacc cgtaatacag aggtccacaa aattccccat ggtcattgat
    10081 tttcaagctc atctatttcc cgaagagtac atcaacgagc ttaagcgccg cgacggagcg
    10141 gtgattctag aagagcccga tccatacagc ggcatgactt atttctacga caagaaattg
    10201 cgatgccgta tcaacaccgc cattttccaa gggcgtgaca tcgagcgccg actcaagcat
    10261 atggacgggc taggcatcga tattcatgtg ttaacgattc cggctcccgg cgcggatcgc
    10321 ttcgaaggcg ccgatgccgt tgcgattgcc cgggccgcaa acgatgcggt tgccgctatc
    10381 tgtcggcaac acccgacccg ctttatcggt ttttttacct taccgaccag ctcgatttcg
    10441 gcgtcgttgg atgagcttga gcgttctgtg catcagttgg gactcagggg ttttggttgt
    10501 ttctctaatc ttaatggcca agctctggat cgtgaggaat tgtttccagt ttatgagcgg
    10561 ctggcgaagt ataagttgcc ggtttacatc catcctacgg cgccattggc tagtgaagct
    10621 acaggaatgg acattatgcc gaccttgata ttcggctggg ctttcgacag cacggtggcg
    10681 atgacccgac tggtgtatgg ccgcgtgctg gagaggtttc cggaaatcaa tttcgtcgtg
    10741 gccgacgtcg gcggcgtgct ggcatttttc gcccagcgcg ccaagaatat ttactccgga
    10801 cgcactgaag agatccggca acggtacggg ctcaaagaga acccgctgga ctcttttcgg
    10861 cgattctatg tcgacaccgc ggatcatccg gccgcgacat tgaaatctgt tttggaattt
    10921 tttggtccgg agcggctcgt gcttggaacc aattatccct atggtcccga ggagggctgc
    10981 gtgctgatca aaaatagttt ggccgctatc gatggtttaa atctaaacaa ggaagacaaa
    11041 gagaaaattc tcggcggcaa tgccgcggag attctcggcg tgggagcggc atgaagttta
    11101 ccaaggcagc gagacccgtg gttatggggc agaacggggt ggtgtccgcc ggacatccgc
    11161 tcggtgcatt ggccgggatg aagattcttc aagagggcgg caactccgtc gatgccgctc
    11221 tagcggctgc cttcgtcatg tcagtcgtca agccggaggc cagcgggcct ggcggtgatc
    11281 tgttcgcctt agtttatatg aagaaggctg gaaaggttat ggcgctaaat tccagtggcc
    11341 cggcgccggc caacgcaacc atcagctact tccaggaaca ggggttgaag agcattccga
    11401 tcaacggacc gctgagcatt gccgtaccgg gcgcggtgga cggctggctg gagcttcaca
    11461 aaaactacgg caccctagaa ctttcaagac tagtgtccga cgcggtgcgc atcgcccgtg
    11521 aaggatttcc gatttcccag gactttgccg aagcgattga ggagcatagc cgggaatctt
    11581 cctgggtcga tcgtttttac cggaagcctc ttggcgtttc caaaccggga aagctattaa
    11641 gccagcgagg cttggcgcga atttttgaga agattgccga tcacgggcgc ggcggattct
    11701 acagcggcga agtcgccgat caaattatcc gaacggtcaa agccgaaggc ggcatcctgg
    11761 ccgaagaaga tttgcagaaa gtcattgcgc agtggatgga cccgctgaaa tcttcgtatc
    11821 gggacactat ggtgtatcag caaccgccgg tatcgcaggg cttcatggtt cttgaaatgc
    11881 tcaacattgc cgaagcgtgg ccgttcgata gcggcagcat gagccgcgca gaaatgattc
    11941 acctccaggt tgcggcaaag aaactcgcat ttgaagatcg cattcgttat ctcgaagatc
    12001 cgagttttgg cgatcccaaa atcggcatgc ttctctcaaa agattatgcc gccgcccttc
    12061 gcgagcacgc cgacgacgcg ctcaagcgaa gcgcgccggc agcccccaat caaagtagcg
    12121 acactaccta tctctgcgcc acggaccgcg atggcaatgc gatctccttg atacagagcg
    12181 tgttcgcgcc gtttggctcc ggggtgatcg ctggagaaac cggcgtcgtt atgaacaatc
    12241 ggttgtgcag cttcggactc gatcccgcta aagcaaactc gctcatgcca ggaaaacggc
    12301 cggcgcatac gctcaatact tatatggttt tcaagggcga tgaggtcttc gccgtcggcg
    12361 gtagtcccgg ggccgacgaa cagccgcaaa ctaattttca gattattcac gatctggtcg
    12421 atctgaagat ggatccgcaa agcgccgttg aagcgccccg atggagccat caaccaggca
    12481 cgccgccgcg agatcagctc ccggagatgc tgcgtatgga agaaggtttc gatgcgcaga
    12541 ccttagaaga tttgcgaaat cgcgggcaca agctgtctgt ggtcgatcgc tggtcattcg
    12601 gcagcgcgaa gatcatcgtc cgcgatcacg agaatggctg ctggatgggc ggtgcggacc
    12661 cgcgcagatt ggcttacgct ctgggatggt agtcctgcag cgccctagac gcgggaattt
    12721 cgatcctcta cctcggtttt taatcgaccg agaatgccgc tcgtagaaca aaccgcgacg
    12781 acatcttcga atattttagc ccgagacagt tggggcaagc ggggtgcccc aggacggatt
    12841 taaggactaa cgtgaagttc agcttgttta cagaaattca gtgcccggta ggttcatctc
    12901 ccgaaaagcg cctcgatgag tttctcgagc aagctgagct agccgacgat cttgatttta
    12961 caacgttctg gatcgccgag attcactgcc agccgaggtt ttctctactt tccgcgccgt
    13021 acgtggtgtt gggagcggta gctcagagaa ccaagcgttt acggctcggc gtcgccgtaa
    13081 ataccttgcc gattcatcat ccggtacagc tggcggaaga ggccgcgatg ctcgacatac
    13141 tttccggcgg tcgcatggat ttcgccgccg gcggcggtca tccacacagc cgcgcctacg
    13201 aatgctttgg cgcagaccac aaaatcacac acgagctcat ggtcgaagct ttggaaatca
    13261 ttcgtcgggc ctggtctgag gagactctga attttaccgg aagatatttt cagattcctg
    13321 aagttatcgt gaacccaaaa ccggtgcaac ggccgctgcc atcattttac atggcgacca
    13381 gttctcttga tggcgtcgca gtgggcgccc gtctcggcgt caatttgctc ctgccgattc
    13441 atacccgcac gccggagcaa gtgatcgaat tttccgaagc atattggcag ggattgaaaa
    13501 ccaacggtca tgatccaaaa tccaaggagt tgggtcttct ggtgccgatg catctggcgc
    13561 cgagtattgc gcaggcccgg tcgcgctccc aagatggcat catgagttac ttcaaaacca
    13621 tcgccgacat gcgcaacgac tatgtcgatt ggttgactcg gcgcggtgat gaattacccg
    13681 tccggctgag aaccactagc ggcgcctcgg tgagtttcaa ggatatctgc gaccggcatg
    13741 ctatagtcgg gaattctgaa tcggccctgc acaggctcaa agctttaacc gcggctaccg
    13801 gtgcgaccaa cctgctcacc tggttcaaca tcggcaacgt gccgcacgcg ctggttcaag
    13861 aatcgatgca acagttcgcg gctgaagtta tgccgcatct gtaatatttt cagggaggat
    13921 cgatatggaa aatctgacgg agcagaaatg cgaagcgtgt cgcgtcggtg ctccgcccgt
    13981 tacagccgac gagatacaag agctccatcc cagagtgtct gagtggctca tcatcactga
    14041 agagaacatt cccaagttgg atcggcaatt caaattcaag aatttcagag aggccctagc
    14101 gtttaccgat gccgtgggcg cagccgcgga agaggagggt catcatccgc gtatcacgac
    14161 cgaatggggc aaagttggcg tgacctggtg gacacacaag attcgcaatc tacacaagaa
    14221 cgatttcatc atggcggcga aaacggacgc gatctacaag aactatccgc agagttaaag
    14281 gatcaagcag cgggagcggg cgatggacgt tcgatgagac tgtccgcgag atagacaacg
    14341 gatacaccct gaacgacttt taccaacaag aagaagagcc ggagatccaa tgcttcaatt
    14401 gatgattttt gcctcgcgtt gaagcggcgt tcagggacag aatgaaatcg agcccgtctt
    14461 catcgcgcgt atgaagcagg tgctggtatt tagctacaat aagttccatc catttcaatc
    14521 gttccacttg ttccgtcgtt aatcaggcct agaattttac gtaccagtca acgaaagact
    14581 gtcggcgatg tcaaggctga atcgtcgaat ggcgggtgaa caatcggagc ggctggaagg
    14641 gttggaaccg acgtgaacta tagagcttga tcgatcacga tgtcaatgtt cttggcgccg
    14701 acctcgaccg gttcctgata attccccatc agatctcccg ccgtccgcgt cgtcggattg
    14761 ccatcgttgt ccaaacggac gcttagattg attttcccgc taaacggcgt tccctgcatc
    14821 atgacgttct ctgcgccgat gacataggac aacggaaaaa ccggctgatc gattttcttt
    14881 accgcgagcg gtggccctcc tccggggccg gcgggtctcg cgatgataaa caagatcgcc
    14941 ttgctgttgg tgactttgac cttcgggtcg agagtgattt tgccgctgac ggtttgccgc
    15001 gaatccggcg acggcgcggc gacgccgtgc ccggaattgc tcccgggtgg cggaaaggat
    15061 gccacgatct tttctatctc tgctctgtcc tcgccgggcg ggatgagctg caacaactgc
    15121 tgtaatacca cgcgcgcgcc gtcatagtct ttcttctcgc gatacaggat cattcctttg
    15181 ccccagagtg ccataggata attcggcgct tgcgccaacg ctcgatcaaa agccatcatt
    15241 gcgccatcgg catgacccgc ctgaataagg atcatgccca tatagctgtg cgcctcaggc
    15301 tgattaggat cggcctctaa aactttcttg aatgcttcga tggctttggg aaaatcctgt
    15361 ttcgcaaagg ctctcttgcc ttcttctaag gcggaacgtg tcgcggcgtt atcgggcgtt
    15421 gtgccggtga ggaagtctcc ggtcatcgag tcctgctccg agctgcgcgg ccgtagtgat
    15481 tgagtcaaca ataggccaag agccaggccg aagaggagaa gcaaggctcc acctgtagcc
    15541 agttgccagc ggcggaaccg aatacggctt ggggcctcct gccgccgggc cggcttatct
    15601 tcaaccggtg ctttggccgg gggctccggc gggagatagc ttagctgttg cagaaccaga
    15661 gtttgcttgg cgtccacttc gcttcttaag ccattgtaat cggcttgcga aagtttgccg
    15721 gattgaaaat cgaattccag ttcggctaac tcctctctga ggcggatgct ttcacgtacc
    15781 agctgcgctc ttgcagtaga atcgtcgcta tccggcactt caacattttt agctttcatt
    15841 tctttgcgta attgagccgc aacgctgggg tcgaccttag gagcgacacg gtgcgccttc
    15901 ctggcggccc aacgccgtgc aagcaagaga gcgacgatgc atccgataat caggacagtc
    15961 agcggaagcc accatagaag ccggttaaaa ccggtgttcg tcggcgtcag cagcacccac
    16021 tcgccgtatt tcgacacaaa gaaatctttg atctcttggg gagacttgcc ggcttgcagt
    16081 tgctcgcgaa cactggctcg catctgctgc gccatttcgg acggcgagtc agcgaccgag
    16141 agattttggc aaaccacaca gcgaagttcg gtagcaacgg cgcgcgtttg ctcttcaagg
    16201 tcaggcgccg gctcggcttg gagctggccg cttaaaaaaa tcaacagatt gaaaattata
    16261 taaaaagcca ttgttctaac gaacggattg aaattcgccc cttccttcat tggcgctgac
    16321 aagaccctgt tgggcttcaa gcaacttctc cgtcaccatc gcggcgtgaa taccaccgac
    16381 gtgtttgtag gtgatccgcc cttgcggatc gataaaaaaa ctttccggga tgccccagac
    16441 cccgtaatcg atagcgatct tgccggacac gtccttgccg ttggggtatg tcacctcgaa
    16501 ttcctttaga aattcgagcg atgcctgctc cgtgtcctgg agcgcaactc cgagaaaaat
    16561 cacgttcttg tctttgagcc tttgccaagc tgcttccagg tctcgggcct cggctcggca
    16621 cggcgggcac cacgaagccc agaaattgag aaatatggct ttgccgcgca gatcttcgag
    16681 ccggagcttg ccgcccttga aaagctcgat ggaaaacggc gtcgctggtt tcgccagcaa
    16741 gggcgaggta atgtagcgcg catcacggct aaagccgtac gctagcaacg ccagaattgg
    16801 cgcaatgatc agtaaggtca gcgcgactcg acgccagcgc atgctaaccg acctctggca
    16861 cgtgcaaacg caaaacgctt tgcaaaaatg ttttcacgct tcgaaaatgt gcggacattc
    16921 gattcattcg atcaggccat cgtggacgta aagctttcta actcggaggt ggcgaccagg
    16981 attcgcgata actgcagatt ttccccgggt ctcggcgaag tagtttgcgc aagagtcaac
    17041 cgaactttag gacaggatgc cgggtcatgt ctcagttgga actggtttgc acataggcca
    17101 aatcgtaatc aaggtcccca gcgcgattac caaaccgcca atccacatcc aaaccaccaa
    17161 cggccgaacc agcaccttca aggtggcttg atttttatcc aagtcactaa aaccggatag
    17221 aatcaaatag atatcctcgc tgggactgct gcgatgcacc gcccgtccga tcggcgattg
    17281 ctggctggga aagaacttga gagcggggga caacagcccg atatcggagt cttgcctgag
    17341 cactcggaag gcaccttcaa cgcgatagtg cgttggctgt tgcgagccgc gtagcccttc
    17401 gaaccgaatt tgataattgc cgatcgaaag actttcattg agcccaagcg tcgcctcgcg
    17461 ttcagtgctg taggtcatcg acccggcaat acccatgatg atcaacacga cgcccaaatg
    17521 tacgaccaaa ccgccatagc gtctttggtt gcgccgggtt agagtaatca ggccgcgaag
    17581 aatgccttcc ttggcaagtc ggtgccgcgc cctcagggcc agagcggtgt cataaacaat
    17641 cgtcatgaca acgaaggcga gcagggtaaa gccgagcagc ggcagaaaat ctttcacctt
    17701 ccaagcgaac aaaaacaaag caaaaatcag cgcgccgata gccggccgga gaaaattgcg
    17761 cttcaaattg tcccatgaag cccggcgcca ggcgataatc ggaccgacgc ccatgagaaa
    17821 caccatgaat agaaacagag gaacggtgac cgaattaaag taaggggcgc cgacgctgac
    17881 ttgcaccccg gcaaccgctt cggataggag cgggaagatc gtgccaagaa agatggtgaa
    17941 gagtgcggaa accagcacga tgttgtttaa caggaaggcg gattcccggc taaccatcga
    18001 gtccaattca ggttgctctt tcaacatatc ggagcggtag gcgagcaatc cgaaggaacc
    18061 taataagacg aaggtgagaa atcccaggaa gaaaattcca accggcccgg aagagaacgc
    18121 gtgaatcgac gaaagaattc cggagcgggt gagaaatgtt ccgaagattg tcaggctgaa
    18181 ggcaatgatg atcaaagaaa gattccacac tttcagcatc cggcgccgct cctgcacctg
    18241 aatggagtgt aaaaaagcgg tggccggcag ccagggcatg aatgcggcat tctcgaccgg
    18301 gtcccatgcc caataaccgc cccaacccaa aacatggtac gaccaccacg cgccgactag
    18361 atttcccgcc gtcaggaaga accaggcgat gatggtccag cggcgggtcg tggtaatcca
    18421 tgcctcgtcc aatttaccga cgatcagcgc cgctatagca aaggcaaagg gcaccgtcag
    18481 cccgacgaag ccggtgtaaa gcaatggcgg gtgagtcatc atgttggcgt cttccaggag
    18541 cggattcaaa ccacgaccat ccggcggaac tggaaagatc gtttcgaaag gattggagac
    18601 aaaggcgatg acggcaagaa agaaaaacga cacaatcgag aagaccatga gcacccatgg
    18661 caaagtttcc ttgtggcggc cgcgataaat ccacgcaacc aacccggaga agattatcag
    18721 aatccattcc cacaggagca gagaaccttc gagggcgccc cataaaccgg tcacgcgata
    18781 ataaaacggc gtagccagag tggtattaaa agccacatag cggacggaga aatccgccga
    18841 aaccagtgca tagatcaatg ccgaggccgc cactgttaca aaaacgaact gcgttgaaat
    18901 aaacttctca atagcagacg aataccttgc gtggccgcaa cgcatggaca cgaccggtgc
    18961 gacgattccg gcggcggcag caaacagagc gattaggatg ctggtatagc ctatggttgc
    19021 gctcacttcg aggtgtactc cttcgcgggc acgaaactgt ggggcgcgcc ttgacggtcc
    19081 tcgtgcgggc tgtactcttc agcgtgcttg gccataatcg ttgacgcttg aaacactccg
    19141 tccttgccga tgcggccttc gacgaccgcg cctgttcctt ctctgaagag gtccggcggt
    19201 actccacgaa aaaagaccgg gaaagtactg gcgccatcgg tgagttcaaa atgataggtg
    19261 agattcttca cgtctttctg cagcgatccg ttcacaacca tgccacccat tcgcaaaaac
    19321 ttattcgccg aggatgtctc gtttgctttg agctcggagg gagtgacgaa ataaaccagc
    19381 gcttgttgca tgccgccgta gatgacatag gacagcgcgg ccagtatgat gaggccgccg
    19441 atcagaaaac gcttattttt tgccatgatc tgaaagctcc tccgttgttc ggagttcgat
    19501 gagctcctcc tccgcgcacc ggtaacggcg cttgagcgca aacagataaa tcacaatgca
    19561 gccccagaca atgccgtagg ctaaaaagac aaatccccag gcgcccacta aagggccccc
    19621 tggctaagcc ggcgagcttt gatttcgctt cgtacctcac caagacgcag gcgaagcgaa
    19681 aggagataaa aataaagcag aagaaatccg acaaaattga tcgccagtgc ggtcagcatt
    19741 gccggatgaa cattctccca tggaagcttg tcaggccgca ggatcgaagg cggctgatgc
    19801 agggtgcgcc accagtatac cgacatgtgg atcaatggga tgtcgagaaa gccgatgact
    19861 ccaagaatcg ccgctgataa tgcggcgcga tcttcatcgt cgatcagcga gcggagcatc
    19921 aaatatccgc tatagatcaa gagcagaatg gccgttaatg tcaatcgcgc atcccaggtc
    19981 caccatactc cccaggtggg cttgccccag attgagccct cgatgatggt taacgcggtg
    20041 aaaagcactc cgagctcagc ggcggcatgc gccaagttat catcccgctt ttcacgtttc
    20101 cataagtaca ggcagctacc gataaaaacc aggaaaaaag aaagataggc ggtgagaata
    20161 ctcggcaaat gaagatacat gatgcgctga acgtcgccct gaagagcatc aggaggcgcc
    20221 tggacaagcc cccagtaaag ccccagtgca atcatgatta aggccaaggc accgaggaat
    20281 ccggcgcccc gtttttgtga ctgctctctt ttcattaaac ttcgatcacc cagtcgaata
    20341 ccaacactcc caaggtcaga aatatcacat caaatccaat gagcagacgc aaccacggaa
    20401 gcgcatcgcc gatttcgccg gcgcgaaaca cagccgccaa gcattggatc gtcgccagaa
    20461 tgaccgggat catcagcgga aagagcacca aaggaagcaa caactcccgg gcgcgagcgc
    20521 gtaaggttaa tgccgacatg acggtgccga gcacgcaaaa acctacggtt cccgataaag
    20581 cgatcaaaaa aacctgcggc attaaggtcc atgtctgcaa attaaacaaa atccagaaca
    20641 gcgggataat gacgagctcg acgaccagca tcaaaatgaa attcaaacac accttggcta
    20701 aaaaaagggc gccgcggtcg agaggagaca gcaacaaggc atccagacat tgattctccc
    20761 gttccgcgcc aaacagttgg gcaagtccta aagttccggt gaacagaaag gcgagccaca
    20821 gaagaccagg ggccaattca gcgattctat ccggcgttag gtcgaaggag aagtggaaaa
    20881 cgaacaacag gagggtgccg aagaaaaaca ttgtcagcaa gctatcgcgc cggcgcaact
    20941 gcaaaaccaa atccttgcgc aggacgaggt aggtttctct gagcaacttc atgaatctcg
    21001 atgcggcgaa aattcctgga aagccttgtc gaaatccggc cagggttgtt ggcgcggcgc
    21061 gttgaaagtc attcgccctc tgtgcaggat gatcgcgcga gtacagatct tcattacgcg
    21121 cgaaacgtga tgcgaagcca gaattacgag attgcccttg cggcagtgag ctttgagaaa
    21181 ttcttcaagc agatcaacgc cgctgccatc gagcactccg taaggttcgt cgagcaacag
    21241 cagacccggt ttgagcaaat tccatttggc gatcgagagg cggcacttca ttccgctcga
    21301 cagggtcgat gccaactcgt cggcgcggga gccaagttcc acctttgaca gcgccgcatc
    21361 gatgctcacg gcatcatggt tctggccata caaggatgtg aaaaactcca ggttttcacg
    21421 cacggttaga tcttcgtaaa gatgggcggc gggcgtaagc aggccgattt gagcgcgcgc
    21481 cgacccatag gcgcgcgagg atggcagcga atcaagctca atcgttccaa ggctcggagg
    21541 cgtcagcccg gcaattagtc ttaataaggt agttttgccg gcgccgttgg gtccgagcag
    21601 agcgacgagt tcgcccggcg gaagatccag attcaggtct ttaagcgccc agaaaaaccc
    21661 gtaagccttg gacaagttct gaaccgttaa gtgcaacgaa ttacctgaga ggagaatcgc
    21721 gaattcatcg gagttgatcg actatagtga tcgaagatca aggattcaag ccggattcgg
    21781 cttgcgtcta tcttggagaa agtcatttga cgattttcac cgatgtccct atgtgttaga
    21841 gtcgcgcgaa tttcgagcca gaatgaggag gagttatgaa ccccgtggtt accgtgaccg
    21901 gcgcctggaa cgcctttgcg accaagatcg cagccttcct gcctgatctt atcgcagcca
    21961 ttttcatcgt cgtatttggt tggattctgt gcaacatcat caagcgcatc gtcattcgtg
    22021 ttctgcgcct ggttcatttt gatacggtcg ctgagcgcgc cggcatcaag cagattcttg
    22081 aacgcggtgg aattcagcaa aatccctccg agattcttgg cctgctattc ttctggttcc
    22141 tttttttgat cgtgattgtg gctactctag agaccctcaa cctcagcggc gccaccgaca
    22201 cgctcaatgc catttatctg tatcttccaa aaattgccgc ggcaatcgtc atgttgattc
    22261 tcggcttgta tttcgccaat tttctcgaaa ccgttacccg aacgtcatgc gccaatgcgg
    22321 gattagagca agccggctct atcggacgcg ccgcttacgt tattacgacg atcttcgttg
    22381 ttgcagggat cttcgaaatt ctcgatatcg cctccgaaat tgtcatttgg gctttcatct
    22441 tagtctttgg agcggtctgc ctagcgttgg ctttagcctt cggtctggga gggagagatg
    22501 ttgccggccg ttatctggaa aaatggttgg agcagaaaaa taatgatgag accaagttaa
    22561 tgcaccccta atattccgcg cggtgtttct gatcctccta tatcgctcgt ctaaccgccg
    22621 ctcgatcgct tattcaggtc attagtgcca atccccgtgg atcaattctt gactccctcc
    22681 aatcaatcag ataacttcga tcagttctaa attttcgagc aatgcctaag agttaagtca
    22741 aactcttagg catttttaca ggtgagggag gctatggcag aatacaaagt ggtagaagtc
    22801 cctaaggacg gcgcgaagat caccatggga gccaacaagc agcttcaggt tcccgacaat
    22861 ccgatcattc cattcatcga gggcgacggc accggccgcg atatctggcg cgcctctgtg
    22921 cgcgttttcg atgccgcggt tgcaaaggct tacaagggca agaagaaaat ccgctggatg
    22981 gaagtctacg ccggggagaa atccttcaag caatacaata gctggctaca cgaggagacg
    23041 gtgccggcct tttcggagtt tctcgtgggc attaagggcc cgctgacgac acccatcggc
    23101 ggcggcatga cttcgctgaa cgtcggattg cgcaagttgc tcgatcttta cgtctgccaa
    23161 cgtccggtgc gttatttcaa cggcgtgcct tcgccgctca agcaccccga gtatgtcgac
    23221 atggtcgtgt tccgtgaaaa caccgaggat atctataccg gtatcgagtt tcccaacggc
    23281 accgaaccca atgccaagtt caagtcgctt ctcaaagaga cctttcccaa agaatttgcc
    23341 aagattcgct ttcccaacac cgcagcaatt ggcttgaaac cggtctcggt cgaggggaca
    23401 gagcggctcg tgcgcgcggc gatccagtgg gcgctcaaga ataagcgtaa gaacatcaac
    23461 ttcgtgcaca aaggcaatat catgaaatac accgagggcg ctttcgcgga gtgggggtat
    23521 gccctggcca aacgcgagtt ccgcaacgat gttgtcaccg agcgcgagtc gtggattctc
    23581 ggcaacaagg aagccgacgc taatgtgagc gcggaagcca atgccaaaat gatcgatccc
    23641 ggcttcgata tgatgtctcc ggcgcagcaa aatgatatta agaaagaagt ggaagaggcg
    23701 ctcaagcttt ggccgacgca tggcgacggc aaatggaaga aaaagctgat gctgaaggac
    23761 tctatcgctg acgtcacctt gcagtttgtg ctgattcgtc caaaggacta cgacgtgatc
    23821 gcgacgatga acttgaacgg cgattatctc tccgatgcct tggcggcgca ggttggcggc
    23881 atcggcatag ccccaggcgc gaatattaac tatgacagcg gccatgcggt gtttgaagcg
    23941 acccacggga ccgcgcccaa gtatgccgat ctcgacaaag taaatccggg atcggtcatt
    24001 ctttcgggcg agatgatgtt gcgttatatg ggctggaccg aagctgccga tctgatcatt
    24061 aaaggtatcg agggcgcggt taacgccaag acagtgacct acgattttga acggctgatg
    24121 ccgggctcac agctggtgag ttgctcgggc tttggcgacg ccatcatcgc caatatgtga
    24181 gcgggccgtg ttggggaatg ctgttattct tggttgaatg attcgaggat tttatgacta
    24241 ggaacaaaat agcgttgatc ggcgccggta atatcggcgg ttcgatggct cacctttgct
    24301 tgctcaaagg tttgggtgat gtggttcttt tcgatgtcgt ggagggattg ccgcagggca
    24361 aggcgctcga cctatcccat gcaggtccgg tcgaaggctt tgacggcaac gtcatcggca
    24421 ctaatagcta tgaagacatc gctggcgcca acgtttgcat cgtaaccgcg ggaattgccc
    24481 gcaaacccgg catgagccgg gatgatcttc tcggcaccaa cgcgaaaatt ctgacatcgg
    24541 ttgccgacgg gataaaaaag cacgccccga attctctcgt cattgttgtg accaatccgc
    24601 tggatgcaat ggtcacattg atgaagcggg ccaccggttt tccaaaaaac cgagttgtcg
    24661 gtcaatcggg catcttggac tcctctcgtt accgatcgtt cattgcccgg gaactcaatg
    24721 tttcggtgaa tagcgttgcg gccatggtat tgggcggtca tggcgacgac atggttccgg
    24781 ttcgcagcac ctgtattgtc ggcggcgtgc cggttgaaaa gctgattccg agcaagcgcc
    24841 tcgacgaaat cgaagccaga gtgcgcaatg ccggaggcga ggtggtggcg ttgctgaaga
    24901 ccggatcggc ttatttctca cccgcatcgg ccgctatccg catgaccgaa gcttacctgt
    24961 ttgataaaaa agaaatttta ccctgcgcgg cattgcttga aggcgagtac ggtgtcaatg
    25021 gttattactt ctgtgttccg gtcataatcg gcgccggcgg cgtcgaaaaa atcgtcgaaa
    25081 tcgatttgca cgcggcggac agaagagcgt tcgatgagtc cctgaatcac gttaaagata
    25141 tcgttcaagc gatggaccgt gtcgtgggcg cttgatcttt gtttgcgggt caaacgataa
    25201 cgagaccctg agacttttca ttagtcttag ggtctcgttt tgttgcgggc ttagattatg
    25261 aatatccatg aatatcaagc caaagagatc ctcaagggtt acggcgtccc ggttcctcgg
    25321 ggcgaagttg ccgcaacgcc ggaagaggct aaggcggctg cgattaggct aggcggcggc
    25381 acctgtgtgg ttaaagccca gattcatgcc ggcgggcgcg gcaaaggcgg cggcgtgaaa
    25441 ctggctcgca accctgaaga ggctgaacaa atcgctcgtg agatgatcgg caaaaatctc
    25501 gtaacgcatc aaacaggacc tgaaggccgt caagtcaggc gagtgctgat cgaacaggga
    25561 ttaaacatcg atcgcgagct ttatctcgct ttggttctcg atcgcgccca gtcgcgggtt
    25621 tcgatgatct gttcttccga aggcggtgtc gagatcgaag aggttgccga aaaaaaccct
    25681 gaaaagatct tgaaagagac tatcgatccg ataatcggcc tggctggttt ccagtgcagg
    25741 cgccttgcct atggcctgaa cattccgggc gagctgatcg gcaagatgac cgctgtcatg
    25801 caggccctgt atcgcgcctt cgacgatgcc gattgttcca tcgctgaaat aaaccctctg
    25861 attataacca aggagggcca ggtcatggcg ttggacgcca aaatgaactt tgacagcaat
    25921 gcgctatatc gacataaaga catcgtcgct ctgcgtgatt tgaacgagga ggaccctcgc
    25981 gaaatcgaag caagcaagtt tgacctgtcc tatatctctt tggacggtaa tatcgcttgc
    26041 atggttaacg gcgccggtct ggcgatggcg accatggata tcatcaagct atgcggtggc
    26101 gagccggcca attttttgga tgtaggcggc ggcgcttcca aagaaaaagt tgcccaggca
    26161 tttaaaatcc tgctcgccga tcctcgggtg cgcggtgttt tgattaacat tttcggcggc
    26221 atcatgcgct gcgatgtgct ggctcagggt atcgtcgatg cggctcatga gatgaacatt
    26281 caagtgcctc tagtggtgcg gatggagggc accaacgtcg ctcagggcaa gaagattctc
    26341 gccgattcag gtttggcgat catcccggcg gaaacgatgg cgcaagcggc agaaaagatc
    26401 gttgccgccg tcgagaagtg atagtctgta gcaggtcgat gacaagtgcg aatttgcagg
    26461 ctgctcaaaa agttagagcc gacgcgcgcg aggaatccgc gagcgcagcc gcacttagcg
    26521 agtacgttgt aacgagacga tcgagcgcaa tggaagatat gagccttttt cagcaacctg
    26581 ctaggtaatt ttaaatggct attctcgtaa acaagcattc ccgagtgtta acccaaggaa
    26641 tcaccggcgc caccggacag tttcatacca aagcgtgcaa agaatatggc actcagatgg
    26701 tcggcggcgt aacccccggc aaaggcggca cgaactttga gggaataccg atcttcgata
    26761 cagttaccga ggccgtggcc gagacggcag caactgccac ggtcatctat gtgccgccac
    26821 cgtttgccgc tgatgcgatt ctagaagcgg ccgacgccga cattcctttg attatctgta
    26881 tcaccgaagg cgtgccggtg cgcgacatgg taagagttaa acggtacctg agcggtaaga
    26941 cctcgcagct gttgggaccc aattgtcccg gcgtgattac acccggcgag tgcaagatcg
    27001 gcatcatgcc cgggcatatc catcgacagg gaagcatcgg tgtggtttcg cgcagcggca
    27061 ctttgactta tgaggccgtc gatcagttga ctcgtttagg tttgggacaa tcgacctgcg
    27121 taggaattgg cggtgatcct gtgcatggtt ttgaattcgt cgatgtgctg gacctgttta
    27181 atcgcgatcc cggcacccaa gccgtcgtta tgatcggcga gatcggcggc agcgccgaag
    27241 aagacgctgc cgcatggatc aaagccaaca tgaaaaaacc ggtgatcggc tttatcgccg
    27301 gtcaaacggc gccgcccgga aaaagaatgg gacacgccgg cgcgattatc tccggaggga
    27361 aaggcaccgc cggtgaaaag attgaagcga tgaaagcggc aggaattaga atgtcggact
    27421 cgcccgcaac catcggccaa acaatgcagg aggctctgag aagtcatgag tgaagtaacg
    27481 ctgtcgatta tcaagcccga tgcggtgcag cgaaatctca tcggcgagat cctccggcgc
    27541 tttgaatcgg ccgggttgaa agtcgttgcc ggtaagttgc tccggctctc ctccgagcaa
    27601 gcgcaggcgt tctatgcggt gcacaaggag cgcccatttt ttgcaggctt gtgcgcgtac
    27661 atgtcttcgg gtccgattct tgtttcggtg ctggaaggca agaacgcaat cgccaaaaac
    27721 cgggagatca tgggcgccac cgacccagct aaagccgccc cgggtacaat tcgcaaagat
    27781 tgtggcaccg acgtcgaagc caacgcagtg cacggttccg atggcccgga aacggccgcc
    27841 accgagatcg ctttcttttt caaaccggaa gaggtcttta gatcttccaa gtaactctcg
    27901 gccaatttac gagcggtcgg tgatgaagcc aaacatcaaa gatttgagct tcaacgaatt
    27961 tgaagcctac ctgttcgagc gtaagcaccc ggcctaccgc gcgcgccagg tgtggcagtg
    28021 gctgtatcag aaacgcgctg cttcgttcgc cgatatgacc aatctctccg gagtttttcg
    28081 agagcagcta gcggctgatt ttagcatcag ccggctggcc gttgagcgta gggcggattc
    28141 ctccgatggc acggtgaagt ttttgttccg attggctgac gaccgcaaca ttgagagcgt
    28201 gttgattccg gaagcaaagc gactgaccct ttgtatttca actcaggtcg gctgcggttt
    28261 tggttgcgca ttttgtgcca ctgctcttct gggcctcaag cgaaatcttc gggccagcga
    28321 aattgtcgat cagatcctcg aagctagccg gactctgact gaagaccggc gcattaccaa
    28381 tgtcgtgttg atgggcatgg gagaaccgtt ggcgaattac gctcaaacca ttaaagccct
    28441 ggcggtgatg accgacaaca gctggggcat cggtatttcg ccgcgacgta ttaccttatc
    28501 cacggttgga ctggtgccgc agattcgcaa gctgatggat gaaaccaaag tgagtctggc
    28561 gatttcgctg catgccgcca ccgatgagtt acggagccgg ctcatgccga tcaatcgcaa
    28621 atactcgctg cggcagctga tggagtgttg ccgggctttg ccgatcccgc gacgcaagcg
    28681 cataactttc gagtacgtgc tcttgcacgg cgtcaatgat tcggccgcgg acgcttataa
    28741 cttggtggaa gtattgcgcg gtgtccgctg taagataaac ttgattcctt tcaacgcgca
    28801 tcagggcagt gcgtatgaac ggccggaaac aaagaatgtc gaggctttcc aacagatcct
    28861 ttcctgtcaa gggctgcagg tcaatgttcg ccgtcccaga ggcgacgata ttgccgccgc
    28921 gtgcggccag ttacagggtg aaacgatcaa agagcaagaa agcgcggccg gtcactgctg
    28981 aaatcgtcga acaacatgag caaacaagtc atcggtgtga tcggcgggag cggcctttat
    29041 cagatagaag gcttgcagcg cgtcaaggaa gttgatctca acactccttt cggaaaacct
    29101 tcggataaat ttattcgagg atggctgggg gacacggaat tgctgttcct gccgcgccat
    29161 ggcaggggcc accgctggtt acctaccgag ataaactttc gcgccaacat cttcggcatg
    29221 aagaagctcg gtgtcgaacg gataatctcg gtgagcgcgg tgggcagcct gcaggaaaag
    29281 atcgcccccg gtcacgtcgt catacccgat caattcatcg atcgcacgac acaacgcccg
    29341 agcacgtttt tcggcaaagg tatagtcgct catgtcagcc tggccgaccc gttctgtaag
    29401 gaactctcgg cgacattggc gaaggcggcg atggtagcgg gcgctaccat tcaccagagg
    29461 ggcacgtacc tttgtatgga ggggccgcag ttctcgaccc gcgcggagtc gcatctttat
    29521 cgcaactggg gcgccgacat catagggatg accaacctgc aggaagcgaa attggctcga
    29581 gaagcagaga tttgtttcgc cactttggcg ctggctaccg attacgattg ctggaatgaa
    29641 tcggccggcg atgtggaaat cgagcatgtc ctggcggtat tgcagcagaa cgtcgatttg
    29701 gccaagaaga ccatccgcca ggtgatcaag aatctcagcg ctgcacgcag ctgtggctgc
    29761 gccctcgcct tacaaaccgc gatcattacc gagaaatctc aaattcccaa gaaagtacgg
    29821 cgcgatctgc gccctattat tggcaaatat ctatgagcat cttagtcgtt ggaaccgtcg
    29881 ctttcgattc gattgagacc ccactgggaa ccgccgagcg cattctcggc ggttccgcat
    29941 cctactttgc gctgggcgcg agttttttcg cgccggtgcg gatcgtcggc gttatcggcc
    30001 aagactttcc gcaagaatat ctggatttgt ttaggcagcg gcaaatcgat cttgaaggca
    30061 ttaaaaaggc cagcggcgat accttttact ggcggggcag gtatcacgaa gacatcaacg
    30121 tgcgtgatac gttggagctt catcttaacg ttttgtcggg tttcgtgccg aacctgccgg
    30181 agcagtaccg tgacgccgat tatgtgtttc tcggcaacat cgatccgttg atgcagctcg
    30241 aagtcctcaa tcagattcgc cgcatgcggc ttgtcgtttg tgacacgatg gatcactgga
    30301 tacgggaaag cctggacggt ttgcgccagg tgctcaagcg catcgaaatg ctggtgatca
    30361 atgattccga agcacgactg ttaagcggcc agagcaacat cgtgcgcgcg gcgcgggata
    30421 tactgaaaat gggaccccaa gtcgtactcg tcaaacgcgg cgaatacggg gtgctccagt
    30481 tctcgaactc atcgattttc gccaccccgg cttacccttt ggaagaagtg tttgatccca
    30541 ccggcgccgg cgattctttc gccggaggct ttctcgggca attggctcgt tctggagagc
    30601 tgactcagca gggaattcgc cgggcgattg tctatggcag cgtcgtggcc tcttttacgg
    30661 tcgaagattt tggggtcaaa cggctggcca agatcacgct gcaggatatc gaagaacgct
    30721 accgaagttt cgcacagctc accgattttc atacctgatc tgatggatct ttcgatagtc
    30781 atcccggtct tcaacgaaga ggaaaacgtc gagccgctca tctccgaaat tgaggcggcg
    30841 ctaaattctc taagcaagca ttatgaaatc gtcgtcatcg acgatggcag ccgggataat
    30901 actttccaaa ttcttgcaca gatctatcgc accgatcccc atctccgggt tgtcaaactc
    30961 aaacggaact ttgggcaaac cgcggccttg gcggcgggcc ttgcgcatgc aaggggcgaa
    31021 gtagtggtga tgttggacgg ggatggccaa aatgatcctg cagatattcc agccttgctg
    31081 gtaaaactgg agcagggcaa cgatattgtc tgtggttggc gctatcgtcg gcgcgacccg
    31141 tttctcacgc gccgcctgcc atcgatgatc gccaattcgc tgatttcctg gacgactcgg
    31201 gtaaaactcc acgactacgg ctgcacgttg aaggcgatgc gcaaagagat tgcaaaaaat
    31261 ctgaagcttt acggtgaaat gcaccgtttt atccccgcca ttgcgtatga acgcggcgca
    31321 aaaatcgccg aagtgcaagt caatcatcgg ccccgattgc gcggcaaatc gaagtacggc
    31381 attacccgaa ccgtgcgggt cattttagat cttatcacgg tcaaatttct catgagctat
    31441 tcgacccggc cctcccatat cttcggaatt ctgggtatcg gcagcggcgc gttgggattc
    31501 atcctggcga tttatctcac ggttcagaag ttgatgtata atgagtcgat cggcaccagg
    31561 ccacttttgc tgctggcggt aatgctgatc tttgtcggct ttcaatttgt caccatgggg
    31621 ctcctgggag agatgttgtc ccgcacctat catgaatcgc aagacaaagc cgtattcgtt
    31681 gtcggggaaa ttcttggcga gaggagctgt gtatgaaact cgcggtcatt ggcaccgggt
    31741 atgttggatt ggtagcgggg acctgttttt cagaaacggg caatgaggtt gtctgcgtcg
    31801 acatagatga ggccaagatc gtaatgctcc agcaggggaa gattcccatt tacgagcctg
    31861 gattggaaga gatggtgcgg cgtaacgccg aagagcaacg tctgatattc accacagatg
    31921 tgaaatcagc ggtgcaaaac tcctcgatca tttttatcgc cgtgggtacg cctcaagggc
    31981 tcaacggtca tgctaatttg gaatatgtcc gtaacacggc aaaaagcatc gcccaagcga
    32041 tggatggctt caaaatcatc gtcaccaaga gcactgttcc ggtgggcacc gcggatcaga
    32101 ttcggcaatg ggtgtccgag gaaacatccc aaccgtttac cgtgctgtcc aatcccgaat
    32161 tcatgaagga aggcgcagcc gtag
//
LOCUS       DASUVS010000393         5004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1265178, whole genome shotgun sequence.
ACCESSION   DASUVS010000393 DASUVS010000000
VERSION     DASUVS010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265178"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..938)
                     /gene="ygiD"
                     /locus_tag="VF182_21070"
     CDS             complement(159..938)
                     /gene="ygiD"
                     /locus_tag="VF182_21070"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HEX6439622.1"
                     /translation="MANVMPAIFFGHGNPMNAVTHSSYTEGWRTIGEQTSKPKAILSI
                     SAHWFVPETAVTISAAPRTIHDFGGFPRELYELQYPAPGDPELARRVQELLAPLPVKL
                     DAAWGLDHGTWSVLRHVYPDADVPVVQVSIDETQPAAFHFDLGKKLAALRDEGVLIIG
                     SGNLVHNLHAYAWGQRIVDPYDWAVRFETQARDMMLAGQFQPLIDYEMLGRDAMLSIP
                     TPDHYLPLLYVLGAGRQGERISFPVEGVDGGSVSMLSVRLG"
     gene            1199..2590
                     /locus_tag="VF182_21075"
     CDS             1199..2590
                     /locus_tag="VF182_21075"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX6439623.1"
                     /translation="MSAAGESSNNRLDPPVNGRSDHTLGDANAPITLVEYGSYNCPFC
                     RGADEIIAGLRDRFGDRLRYVFRHRPISGDAVARRGAELAQYAYETTGEYWPAHAALM
                     KRGPTLRPEDFEELATELGLPPTDGLHQEAWRRAQTKVQEDIDSAWRIGARVSPTFFI
                     NNRRYEGPWDESALTEALLGSLGHRVQTAALDFARWAPSTALLLLTMIIVALIVTNSP
                     AAQAFEALWEMPVGWIAGGAAFQLPLRDWLNDGLLTIFFLVVGLEIKRELTVGRLSTA
                     RVAALPLAAAAGGMLLPASLYLLVVPPGPFAAGWAIPTTTDTAFAIALIALLGPRGPV
                     ELRIFLTAAVVVDDLVATAILALFYSAGIHLQYTAAAIGTTAVMVMFNRSGIYAPLPY
                     ALLGIVLWGCLHGAGLHPTLAGVIVASMTPTRPPGNLRALIAQAETVMGHELRFGLGQ
                     EQSNDVNQQRALS"
     gene            2631..4088
                     /gene="cls"
                     /locus_tag="VF182_21080"
     CDS             2631..4088
                     /gene="cls"
                     /locus_tag="VF182_21080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEX6439624.1"
                     /translation="MLMRHIFNHLATLLTLYALVMSVFVIRENRRPQSTLAWMLVLFF
                     APGIGLLIYLFFGRDGKAFSKRSKLLRQDLEANARPLLSPILSRQEQEMGRLAQQSLV
                     RKKLAMLVRRNSISALTTRNQVEIQQDAAKFYPSLIEDLKTARHSIHLQYFIWGVDPF
                     TDGLKEILIDKVSAGVEVRLLYDPQGSQAHVGRRYVKELRAKGIRMAPTSPRYHLHTI
                     SYRNHRKITVIDGTIGYTGGMNIGQEHLDGGKGFDSWRDTQVRLVGESAAVLQAVFMV
                     DWYNAVRENLFQAKYFPTDAAEPVESNIPVQILTSGPDSQWAAIRQLYTLMVISARRH
                     VYLQSPFFLPDATLAEALTEAALSGVDVKVMVSARPSGNRLPDWAGNTYIEDIVTSGV
                     RVFLYQKGYLHAKTISIDSEVCSIGSANIDIRSFSINYELNAVFYSEQIAKELEADFE
                     RDLAYCMEFDPEEYRKRGTVARFRDSVARLLSPLL"
     gene            complement(4194..>5004)
                     /locus_tag="VF182_21085"
     CDS             complement(4194..>5004)
                     /locus_tag="VF182_21085"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX6439625.1"
                     /translation="EGGAGTAVARGRLAERGLENTQVIWRQQPKVSGSNHFGARIVFR
                     RDGTLFVTLGERFNYSDSAQDLSTTIGKIVRIKPDGSAPNDNPFVGRAGARPEIWSYG
                     HRNVQAAALHPESGQLWTVEHGARGGDELNHPEAGKNYGWPVISYGRHYSFLPIGEGT
                     AKDGMEQPVYYWDPVIAPSGMVFYTGDLFAAWKGNILVGSLTPGGLVRLIMKDGKVAQ
                     EERYLGDLGERIRDVRQASDSSLYLLTDARDGRILRIAPANNASGQESRPR"
BASE COUNT         1009 a         1500 c         1467 g         1028 t
ORIGIN      
        1 gcttctccaa gacctggggt tattcgactc gaagacgccg ccgggcagcg cttcagggtg
       61 ctgaggactc gtaacgcgat cgccgcaacc cggcaagtac aggtgaaagc agcgccgcgc
      121 tcttatcctc cgccaggccg agcgaaagca ccgcgcaatt atccgaggcg gacagacagc
      181 atcgacacgg atccgccgtc cacgccttcg actggaaaac tgatgcgttc accctggcga
      241 ccggcgccga gcacgtagag caacggcagg taatgatcgg gggtcggaat cgacagcatg
      301 gcgtcgcgcc cgagcatttc gtaatcgatg agtggttgaa attggcccgc caacatcatg
      361 tcccttgcct gcgtttcgaa ccgcaccgcc caatcatacg gatcaactat gcgctgtccc
      421 caggcgtagg cgtgcagatt atggaccagg ttaccgctgc cgatgatcaa aacgccttca
      481 tcacgcagcg ccgctagctt cttacccaaa tcgaaatgaa aggcggcggg ctgggtttcg
      541 tcgatactga cctggaccac cggaacatca gcatccggat acacgtgccg cagcacggac
      601 catgtgccgt gatcaagtcc ccatgcggcg tcgagtttca ctggcagcgg cgccagcagc
      661 tcctgcacgc gccgcgcgag ctccggatct ccgggagcag ggtactgcag ttcatagagc
      721 tctcgcggaa agccgccgaa atcgtgaatg gttcttggcg cggcgctgat cgtcacggcg
      781 gtttccggga caaaccagtg agccgagatt gacagaatgg ccttcggctt ggacgtctgc
      841 tcgccgatcg tccgccatcc ttcggtataa ctactgtggg tcacggcatt catagggttg
      901 ccatgaccga agaagatcgc cggcattacg ttagccataa ttttagatat cctccgctcg
      961 aaggaaatta gttcgatgcc agtcgtgtgg aacgccaccg acgattccac ggctgacatg
     1021 attcaggcta tcaaatgccc ctataataaa aatcatgacg aaaaaatatt agacttttaa
     1081 aagctactat gctttcgcta acggaatgcc cccgcaaacc gaaacgtaca ccgcccagca
     1141 catcgatgac aaaagaacgg actgacccgg catcagcgat tgctgcagtg ctacagccat
     1201 gagcgccgcc ggcgagtcat ctaacaatag actcgatccc ccggtaaatg gccgttccga
     1261 tcacactcta ggcgatgcca acgcgcccat caccctcgtc gagtacggca gctacaactg
     1321 cccgttttgc cgcggggccg acgaaatcat tgccggtttg cgcgatcgct tcggtgatcg
     1381 gctgcgctac gtcttccgcc atcgcccgat tagcggcgac gcggtggcgc gacgaggggc
     1441 cgagctggcg caatatgcct atgagaccac cggcgagtac tggccggcgc atgccgcgct
     1501 gatgaaacgt ggcccgacac ttcgacctga ggattttgag gagctggcaa ccgagctcgg
     1561 cctcccgccg actgatggcc ttcaccaaga ggcctggcgc cgagcgcaga ccaaggtgca
     1621 ggaggatatc gacagcgcat ggcgcatcgg tgcccgggtg tcgccgacgt ttttcatcaa
     1681 caatcgccgc tatgaaggtc cgtgggacga gagcgcgctg acggaagcct tgctcggctc
     1741 tctcggtcat cgcgtgcaga cggcggcgct ggacttcgcg cgctgggcgc cgtccacggc
     1801 tttgctgctg ctgacgatga tcattgtggc cctcatagtg accaactcgc ccgcggccca
     1861 ggccttcgaa gcgctttggg aaatgccggt tggatggatt gccggcggcg cggcatttca
     1921 gctgccactg cgagattggc ttaatgacgg cttgctgacg atttttttcc tggttgttgg
     1981 gctcgaaata aagcgcgagt tgacggtggg acgtttgtcc acagctcggg tagccgcgtt
     2041 gccgttggcg gcggccgctg gcggcatgct gcttcccgca tctctatacc tcctggtggt
     2101 gccgccaggc ccgtttgccg cgggatgggc gatcccaaca accaccgata cagccttcgc
     2161 gatcgcgctg atcgcgctgt tgggcccgcg cggtccagtg gagttgcgga ttttcctgac
     2221 tgcggcggtt gtcgtcgatg atctcgtcgc gactgcgatt ctggcgctgt tttactccgc
     2281 tgggatccat ttgcaatata ccgccgcggc cattggcacg acggcagtga tggtcatgtt
     2341 caatcgcagc ggcatctatg cgcccctgcc ttacgctctg ctcgggatcg ttctgtgggg
     2401 ctgcctgcac ggcgccggtc tgcatcctac cctggccggc gtcatcgtcg cctccatgac
     2461 cccgacgcga ccgccgggaa acctccgcgc gttgatagca caggccgaga ctgtgatggg
     2521 ccacgagctg cgcttcggac ttgggcaaga gcaatcaaac gacgtcaatc agcaaagggc
     2581 gctaagctaa aggtcatagc catgacatcg ggcgcggcgg cgagctgtcc atgctcatga
     2641 ggcatatctt caaccacctg gcgacgttgc tgactctcta tgcgttggtt atgagcgtct
     2701 tcgtgattag ggaaaaccgc aggccgcaat cgaccctggc atggatgctc gtgctctttt
     2761 tcgctcctgg aatcgggctg ctgatttatc tttttttcgg cagagacggg aaagctttca
     2821 gcaaacgcag caagttactc aggcaagatc tcgaggccaa cgcgcggccg ttgttgtcac
     2881 ctattctatc gcgtcaggaa caggagatgg ggcggctggc gcagcaaagc cttgtccgta
     2941 aaaagctggc gatgctggtg cgacggaact ccatttcggc gctgacaact cgcaaccagg
     3001 tcgagatcca gcaggatgca gcgaaatttt accccagtct catcgaagat ttgaaaactg
     3061 cgcgccactc gattcatctc caatacttca tctggggcgt ggacccgttc accgacggct
     3121 tgaaggaaat cctgatcgac aaggtgagcg cgggtgtcga ggtgcggctg ctctatgatc
     3181 cgcaaggctc ccaggcccac gtgggccggc gctatgtcaa ggaattgcgc gcgaaaggca
     3241 tccgaatggc gccgacatcg ccgcgctacc acctgcatac gatcagttat cgcaatcacc
     3301 gcaagatcac cgtgatcgac ggcacgatcg gctataccgg cggcatgaat atcggccagg
     3361 aacacctcga cggcggcaaa ggatttgatt cctggcgtga cacccaggtg cggctggtgg
     3421 gagaaagtgc ggcggtgcta caggcggtat tcatggtgga ttggtacaat gcggtcaggg
     3481 agaatctgtt tcaagccaag tactttccca ctgatgcggc ggagccggtg gaaagcaata
     3541 ttccggttca gatcctgact tcgggaccgg attcgcaatg ggcggcgatc agacagcttt
     3601 acactctgat ggtcatctcg gcgcggcgac acgtctatct tcaatcaccc ttcttccttc
     3661 ccgacgctac cctcgccgaa gctctgaccg aggcggctct atccggtgtc gatgtcaagg
     3721 tcatggtgag cgcgcggccg tcgggcaacc ggcttcccga ttgggccggg aacacctata
     3781 tcgaggacat cgtgacgtcg ggcgtgcgcg tgtttctcta ccagaagggc tacttgcatg
     3841 ccaagacgat cagcatcgac tcggaagtct gctccatcgg ctccgcgaac atcgatatcc
     3901 gcagcttcag catcaattat gagctgaacg ccgtgttcta tagcgagcaa attgccaaag
     3961 aactggaagc ggacttcgag cgcgatctcg cctattgcat ggaattcgat cccgaggaat
     4021 atcgcaagcg cggcaccgtg gcgcgcttcc gggattcggt tgcgcggctc ctttcgccgc
     4081 tgctgtgagg atagttgaca tgtagaagcg cggatctacc ttttgtctct ttcaaaggcc
     4141 agaaatactc gctaggacgg ataaactaaa acgcctcgcc cattttcata ctctcaacgt
     4201 gggcgggatt cttgtcccga agcgttattc gccggcgcga tcctgagaat ccggccgtcc
     4261 cgggcgtcgg tgagaagata gagcgagctg tcggacgcct ggcgcacgtc gcggatgcgc
     4321 tcccctaaat caccgaggta acgttcttct tgcgcgactt tgccgtcttt cattataaga
     4381 cgaaccagtc caccgggagt aagcgacccc accaggatgt tacctttcca cgcggcgaac
     4441 agatcgcccg tgtaaaagac catcccggac ggcgcgatca ccgggtccca atagtaaacc
     4501 ggttgttcca ttccgtcctt ggctgtccct tcgccgatgg gaagaaaaga gtaatgacgc
     4561 ccgtacgaga tcaccggcca accatagttt tttccggcct cgggatgatt cagctcgtcg
     4621 ccgccgcggg cgccgtgctc cacggtccag agctggccgc tctcgggatg gagcgctgct
     4681 gcttgaacgt tgcgatggcc gtacgaccaa atttccggcc gcgcgccggc gcgtccaaca
     4741 aagggattgt cgttcggcgc cgagccgtcc ggcttgatgc gcacgatctt gccgatcgtc
     4801 gtcgataaat cctgggcgct gtcggagtag ttaaaccgct ctcccaaagt tacgaacagc
     4861 gtgccgtcgc ggcgaaacac gatccgcgct ccgaagtgat tagagccgct caccttcggc
     4921 tgctggcgcc agatcacttg tgtgttttca agcccgcgtt ccgcgagtcg cccgcgggca
     4981 acggcagtgc ctgcgccgcc ttcg
//
LOCUS       DASUVS010000394        10458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_609111, whole genome shotgun sequence.
ACCESSION   DASUVS010000394 DASUVS010000000
VERSION     DASUVS010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10458
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609111"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VF182_21090"
     CDS             complement(<1..190)
                     /locus_tag="VF182_21090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HEX6439626.1"
                     /translation="MIRHSPRRLSFLDRYLTLWIFLAMAAGVAVGSVFQGAPRFLNSL
                     SIGTTNIPIAIGLILMMYP"
     gene            complement(187..609)
                     /locus_tag="VF182_21095"
     CDS             complement(187..609)
                     /locus_tag="VF182_21095"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX6439627.1"
                     /translation="MPGGSQKILFLCMGNSCRSVMAEALISHLGQGRYKGWSAGSSPA
                     GYVHPKAIETLKRHGIDPAAPQSKSWDVFANEPFDLVITVCDQAAGESCPVISGKPTK
                     LHWSIPDPAKAQGTEAEINATFDESFTLLKGRIEELVR"
     gene            complement(609..1082)
                     /locus_tag="VF182_21100"
     CDS             complement(609..1082)
                     /locus_tag="VF182_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEX6439628.1"
                     /translation="MKRIHIHVGVEKLDESIRFYSAIFGAQPVKTKADYAKWMLEDPR
                     VNFAISTRASKNGVDHLGIQVEENEELEELRGRIKSADLAVFDEGETVCCYANSEKSW
                     VLDPAGIAWEAYRTMEDAQIFSTKLRGSETACCAEERVSNAPCCEPSEKVSGCCG"
     gene            complement(1079..1450)
                     /locus_tag="VF182_21105"
     CDS             complement(1079..1450)
                     /locus_tag="VF182_21105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007693121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6439629.1"
                     /translation="MDIQQALVAFDALSQETRLKVFRLLVKHGSEGTPAGTVSEALGI
                     PHNTLSFHLSHMSNAGLVLSRREGRSIIYSSNFELITDLIRFMVEDCCRVEFANIRTD
                     KKRGRSVIELFNCCQPKEKKA"
     gene            1597..1974
                     /locus_tag="VF182_21110"
     CDS             1597..1974
                     /locus_tag="VF182_21110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439630.1"
                     /translation="MAQLQQAKKDRLIALLQEFKRLQKELKAIHRTTAYADLEHGVLA
                     LEIAQHTIEETLEHTGLGGEIQRKPNPTAHRKAKEWQKIVKSVRAQGGRFLETHPSED
                     LETALRALAIAEGSLEEVAEHYE"
     gene            1967..3199
                     /gene="chrA"
                     /locus_tag="VF182_21115"
     CDS             1967..3199
                     /gene="chrA"
                     /locus_tag="VF182_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX6439631.1"
                     /translation="MSDGDETAGNKLIRERNRGSLLEVLWVSTRLGLTSFGGPIAHLG
                     YFHEEYVKRKQWIDEQSYADLVALCQFLPGPASSQVGIAIGIARARLIGGIAAWLGFT
                     LPSALVLIVFGLGIGAFAGAADAGWLHGLKIVAVAVVAQAVWSMARSLCPDRERATIA
                     ILATIVTLAWSSAVGQLLSIAIAGIAGLVFFRSTASSTVSHMRFPVAKKTGIAAWIIF
                     FALLTLLPLFRQLAPSHALEVFDSFFRVGSLVFGGGHVVLPLLQAEVVEPGWVTNQQF
                     VAGYGAAQAVPGPLFTFSAYLGTVMGPEPNGWPGALLTLVAIFLPSFLLVTGALPFWN
                     LLRSVPVFQSALKGINAAVVGLLLAALYNPVWISAIYSPADFGLGLIAFALLMFWKFP
                     PWLVVVLTAIGGEVLARI"
     gene            3660..4895
                     /locus_tag="VF182_21120"
     CDS             3660..4895
                     /locus_tag="VF182_21120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX6439632.1"
                     /translation="MKNRNCVTCVLNTKLLKPAPNVYWFLLFGVCIWLGLNGQIAQAI
                     EISSSRDPGDFIEYWASARLLLNGANPYSPEEMLSLQRSIVPDRIDPLLTWNPPWTLF
                     FTAPFGFMTFPVAHLLWLLVNTTVLIICSPLLWKVYGGDRRKARIAWFVCFAAIPTHM
                     VLSLNQITPLLLLGIVGFLIFHSRKLFFLSGFSLTLVAIKPHLAYLFWLALLLWIIDQ
                     RQWSIFYGAMVGVASATLLPLLFVPDIFSFYLAQYSTQLAPRPLDWQTPTLSTALGYL
                     IGSNALWVRTLPTFLGIIWLTIYWSKNKHRWDWVKQMPLLLLTSQVATPFAWTFDQIL
                     LLPALMEVAVWTSHSTRLMPIIKAIVIAFTMINLGPLIVNAIAPYYQWLFWMVPLYLI
                     AYLHLNATEPKLDKSLNPR"
     gene            4904..5662
                     /locus_tag="VF182_21125"
     CDS             4904..5662
                     /locus_tag="VF182_21125"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6439633.1"
                     /translation="MDQDDFIAELWTYASELPTEQHPWFDGIIKHRWTREQIILGEVQ
                     HYLRVRTNPIFFGYMAVNAVSAKEYAVMDTVLENFMEELGGKRTHMDIMLQFLEEGGI
                     SRPEADNAEPAPGTMAAIEMIIGCCQRRSALEGVAMLAFVESQLGGEGKVSEKVYREL
                     TGHYGFSSRAAETYRLHAAQDEGHGSRQIDAIRLLAKDNATQERVRAAVKLGVTAFTL
                     EWDGHVQAMTGKREFWGGVVDLNLRQPLVRFKNP"
     gene            complement(6112..6612)
                     /locus_tag="VF182_21130"
     CDS             complement(6112..6612)
                     /locus_tag="VF182_21130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439634.1"
                     /translation="MSQRFKRFERSTEVGQDLVAGWNRSFSFNDVKTENPSIAVEQVE
                     PSRSENSVLWLFKWRVRNATEIPMSLLSVRVPHGKFRAEERKFGPAVKIPAQDHFIVD
                     QAVACQEPPNTVVENAFLILLVNWQGNDWRVFVRLRITVDQQGRPQTTTESITVQRVG
                     FSGVSD"
     gene            complement(6696..7718)
                     /locus_tag="VF182_21135"
     CDS             complement(6696..7718)
                     /locus_tag="VF182_21135"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439635.1"
                     /translation="MKLRTLSHDFRLLWSVGALLAAMLAQPQPILAQEKKNVRMVFVS
                     LAWNSEIPFRTALARGFFKQQGLEVEPILIRGGPAAIAALVSGEVDFAAIGGAQAVFR
                     SKARGLDLAIVGCTSGTTNYILLGNKQTRTVEDLKGKIIGVTGAGTYSEFAIKAFLKK
                     NNINPDKDVVLRAIGGTVLRAAAIEKGIIAAAPFSTEDGVRLIKAGYSVISNMSESLG
                     IPQNILVTRNEVLEKFPETSKRMLKAYIQGINLAKFNKQEAIKAGYVAGLQGEPEIVS
                     AAWDLYAPGLTSDLSIARAGLQQMLDEDIRAGLVDKNFTLDRVVNDRILKLAQQELRA
                     EGKLKQ"
     gene            complement(7768..8079)
                     /locus_tag="VF182_21140"
     CDS             complement(7768..8079)
                     /locus_tag="VF182_21140"
                     /inference="COORDINATES: protein motif:HMM:NF016130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HEX6439636.1"
                     /translation="MQSNGFKVEKKSLNDKQLLDVKIKNKVPEKLYSCHTALVEGYVI
                     EGHVPADLIQRLLKERPAVAGLAVPGMPVGSPGMEGANPQPYEVISFDKAGRTFVYAR
                     R"
     gene            8378..9508
                     /locus_tag="VF182_21145"
     CDS             8378..9508
                     /locus_tag="VF182_21145"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6439637.1"
                     /translation="MKNGFKILDSDMHLMEPVDLWERYIDAKYKADAPRGLTSDNVRD
                     LRMAHPDGRYWGLPASHNRNSSHHRGHNFNKNQTIYRSHGERGWTATVQLEAMDIEGI
                     DVAVLYPTRGLQVLSEPRMEPKFAAALARAYNDWLYDFCKTDPARLLGAGMLSPFDID
                     EAVAEAKRCATVLGFRAVFMRSSIMEGRNWYDPYFEPLWATLEEYQMPVGFHESSSSA
                     ARQTGDFFEPNFMLRRAVAQPMEQMLGLVSLCSGGVLARHPNLRAAFLEANCTWLPWL
                     LWRLDEGWEREGDIWAQDLAVKPSVYFKKQCFVSVEPDEHGVKYVIDYIGTDRLVFST
                     DYPHGDSKFPDAVGTFLDLPISDEDKRKILWDNCAEFYRLGP"
     gene            9788..>10458
                     /locus_tag="VF182_21150"
     CDS             9788..>10458
                     /locus_tag="VF182_21150"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX6439638.1"
                     /translation="MAEQVFSSTELYDAWPVLSVEDKLEGFKLLPPEERELFFKQLSA
                     RDEANLLEAFPPGERRFWIRLLAPEDAADLIQEAAADEREKMVSIIDEPLRSEIKGLL
                     AYAEDQAGGLMSPRYARLRPNMTVDEAFAYLRRDARDREKKFYYAFVVDPQERLLGVV
                     SFRELFSARGDQSIQNVMRTDIVTARDNMDQEALSDLFARHNLMMIPIVDAGTHIKGI
                     VTHDD"
BASE COUNT         2324 a         2854 c         2757 g         2523 t
ORIGIN      
        1 gcgggtacat catcagtatc aggccgatgg caatcgggat attcgtagtg ccgatcgata
       61 ggctattcag gaatcgtggc gcgccctgga acacggagcc aacagcaaca ccagccgcca
      121 tggcgagaaa aatccacagg gtaagataac gatcgaggaa tgataagcgt cgcggtgaat
      181 gacggatcac cttactaact cctcgatgcg acccttcagc aacgtgaagg actcatcgaa
      241 tgtggcgttg atttcggcct ccgttccctg cgccttcgcc gggtcgggaa tactccagtg
      301 aagctttgtg ggcttacctg agataactgg acagctttcg ccggcggctt gatcacagac
      361 ggtaatcaca agatcgaaag gttcattggc aaaaacatcc catgacttgc tctgcggcgc
      421 ggccggatca atgccatgcc gttttagcgt ctcgatggct ttcgggtgaa cgtagcctgc
      481 cggagaactc ccagcgctcc atcccttgta tcgtccctgg cccaggtggc tgatcagcgc
      541 ttcagccatc accgagcggc aggaattccc catgcacaaa aataaaatct tttgcgaacc
      601 gccaggcatt acccgcagca cccgcttacc ttttcagacg gttcgcagca gggcgcatta
      661 ctgactcgtt cctccgcaca gcaggcggtt tcgctgccgc ggagcttggt ggaaaagatc
      721 tgcgcatcct ccatcgtccg gtaagcttcc caggcgatgc ccgccggatc gagcacccac
      781 gatttttccg aattggcgta gcagcagact gtttctcctt cgtcgaagac ggcgagatca
      841 gcacttttaa tgcgtccgcg cagttcttct agttcctcgt tttcttcaac ttgaataccg
      901 aggtgatcga cgccgttctt ggaggcgcgg gtggagatgg cgaagtttac ccgaggatcc
      961 tcgagcatcc acttggcgta atctgctttg gtcttcaccg gctgagcgcc gaagattgcg
     1021 ctataaaatc ggatcgattc gtcgagcttt tcgactccga catggatatg aatgcgtttc
     1081 atgctttttt ctcctttggc tggcagcagt taaataattc aatgacggaa cgtccacgct
     1141 ttttatcggt gcgaatattg gcgaactcca cccggcagca atcctcgacc atgaagcgaa
     1201 tgagatcagt aatcaattca aagtttgaac tgtatatgat cgagcggccc tcgcgccggg
     1261 acagcacgag tccggcattg ctcatgtgtg ataggtgaaa tgacagcgta ttgtggggaa
     1321 tgcctaaggc ctcgctcact gttccagcag gtgtcccctc cgatccatgt ttcaccagaa
     1381 ggcgaaatac tttcagccgg gtttcctgcg atagcgcgtc aaaggcaaca agggcttgtt
     1441 gaatatccat gtgtccagaa atatggacac gatcaagacc aaagtcaagg ccgtcaaatt
     1501 ggttcgataa tgaacgttct gcaggattga taagacttcg ggcccgctat atcggtagag
     1561 gagtaaaaca ggcttatcga gcgaaaggaa agagaaatgg cgcaactgca acaagctaaa
     1621 aaggaccggc tgattgcact tctgcaggag ttcaagcggc tccaaaagga actaaaggcg
     1681 attcacagga caaccgctta cgcagatctt gaacacggag tgctcgcgct cgaaatcgcg
     1741 caacatacga tcgaggagac tctcgagcat acaggcctgg gcggagagat tcagcgcaag
     1801 ccaaatccca cggcgcatcg taaggccaag gaatggcaaa aaatcgtcaa gagcgtgcga
     1861 gcccagggcg ggaggtttct cgaaacacat ccgagtgagg atttggaaac agctctaaga
     1921 gcgctggcca tcgccgaagg gtcgctcgaa gaagtggccg aacactatga gtgacggcga
     1981 cgaaaccgcc ggcaataaac tcatccgtga acgaaaccgg gggtctctcc tggaagtcct
     2041 ctgggtttca acacgactcg ggctaacctc tttcggcggt cccatagccc atctcgggta
     2101 ctttcatgag gagtacgtaa aacgcaagca gtggattgac gagcaaagct atgcggatct
     2161 tgtggcgctt tgtcaattcc tgccggggcc ggccagtagt caggttggaa tcgccatcgg
     2221 aattgcgaga gcgcgtctca ttggagggat tgccgcctgg ttgggcttta ctctaccatc
     2281 agcccttgtt cttatcgtct ttggactcgg catcggagca tttgcaggcg ccgccgatgc
     2341 cggatggttg cacggtctca agattgttgc cgtcgcggtc gtcgcccagg cggtttggag
     2401 catggctcgc tctctctgtc ccgaccggga acgggcgacc attgcgattc tcgcaacgat
     2461 cgtcacgctg gcctggtcga gtgctgtcgg gcagctctta tccattgcaa tcgccggcat
     2521 tgccgggttg gtattttttc ggagcaccgc ttcttcgaca gtctcgcata tgcgatttcc
     2581 ggtcgctaaa aagacgggaa tcgccgcctg gatcatcttc tttgcgcttc tcaccttgct
     2641 accgctcttc agacagctgg ctcccagcca tgcgctcgaa gtgtttgata gtttctttcg
     2701 cgtcggatca ctggtcttcg gcggcggcca tgtcgttttg ccgctgttgc aagcggaggt
     2761 tgtcgaaccc ggctgggtga ccaaccaaca atttgtcgcg ggttatggtg ccgcgcaagc
     2821 agtgccggga ccgcttttta ctttttccgc ttatctcggc acggtaatgg gtccggaacc
     2881 caacggctgg ccaggcgcgc tcctcactct ggtggcgatc tttcttcctt cttttctgct
     2941 ggtcactggc gctcttcctt tttggaatct gctccgttcg gtgccggttt tccagtctgc
     3001 tctcaaaggc atcaatgcgg ccgtagtggg attgctgctg gccgctctat ataacccggt
     3061 ttggataagc gcgatctatt cacccgcaga ttttggcctt ggcctgatcg cgttcgcgct
     3121 tttgatgttc tggaaatttc ctccttggct agtggttgtg ttgacggcca ttggcgggga
     3181 agttctagca cgcatctgac atcgggaatt aagcagcggt ccggcctgat tcccggaaaa
     3241 tttctaccca ttggcggcat gtttgccccg cgctcatccg cttgcagatc ccagtcagat
     3301 ttgagttcgc ctaaagatca gctcgtgaaa gctaaactgc tatgaaaccc cagttgccac
     3361 ttagctatca tggatcggag gagcgccggc aagccaggca cttcagtcga aaatgataat
     3421 cgttgggctc gattttgtgc cgcttgcgca aaagcaatga tacaatagcg gcacacgacg
     3481 agttcattgc agacaatggc gcgcattgcc gaagcactgc aggtctatgt atgaaagcgc
     3541 aagcctttcg gtttcgatgc ggtcccgtac ctcgagcaac gggcgaacgg tatggcgttg
     3601 ccaagtgagg cctggctatt tggccaaata tagccgtata gaaaattaac cctgattcga
     3661 tgaaaaaccg aaactgcgtg acctgcgttc tcaacacgaa gctcctcaag cctgctccaa
     3721 atgtttattg gttcctgctt tttggcgtct gtatttggtt aggattgaac gggcagattg
     3781 ctcaggcaat cgaaatctcg tcaagccgag accctggaga cttcatagaa tattgggcat
     3841 cggcacgtct tctattgaac ggcgccaatc cctactctcc ggaggaaatg ctctccttgc
     3901 agcgctcaat tgttcccgat cgtatcgacc cgctgctcac ttggaatcca ccatggacgc
     3961 tattttttac cgcgcccttt ggcttcatga ctttccctgt agcgcatcta ttatggttgt
     4021 tggtcaatac gacggtgcta atcatctgct cgcctttgct atggaaagtt tatggcggcg
     4081 atcgccgcaa ggctcgaatt gcctggttcg tatgtttcgc agcgatcccg actcacatgg
     4141 tcctgagtct aaaccaaata actcccctcc tgcttttggg cattgtgggg tttttgattt
     4201 tccatagccg caaactcttt tttctttccg gattcagttt aaccctagta gcgatcaaac
     4261 ctcatcttgc gtacttgttc tggctcgctt tgctgttgtg gatcatcgat cagcgccagt
     4321 ggtcaatctt ctacggcgcg atggtcggcg tagcgagcgc gactttactc ccgttgctct
     4381 ttgtgccgga cattttcagt ttttacctgg cgcaatattc aactcagctg gcgcccaggc
     4441 cgcttgactg gcaaactcct actctgagta ctgccctcgg gtatcttatc ggctcgaatg
     4501 cgctttgggt tcgtacgctg ccgacgttct taggcatcat ctggcttacg atctattgga
     4561 gcaaaaacaa gcatcgctgg gactgggtca agcaaatgcc gcttttgctc ttgacctcac
     4621 aagtggcaac gccctttgct tggacgttcg atcagatatt attactgccg gcgctcatgg
     4681 aggtggctgt ttggacatct catagcacac gattgatgcc catcattaag gcgattgtca
     4741 ttgcttttac gatgatcaac ttggggccat taatagtcaa tgcgatcgcc ccctattacc
     4801 agtggctttt ttggatggta cctctatatt taatcgctta cttgcatctc aacgcgacag
     4861 agccgaaact ggacaagtct ctcaatcctc gataggtgga gccatggatc aggacgattt
     4921 cattgccgaa ctctggacct acgcctccga acttccgacc gaacagcatc cttggttcga
     4981 cggaatcatc aagcatcgct ggaccaggga gcaaatcatt ctcggcgaag tgcagcacta
     5041 tctgcgggtc cgcaccaatc cgattttctt tggctatatg gcggtcaatg ccgtatccgc
     5101 taaagaatac gcggtgatgg acaccgttct ggaaaacttc atggaagagc tcggcggtaa
     5161 acgcacgcac atggatatca tgctgcagtt tctcgaagaa ggcggcattt cacggccgga
     5221 agcggacaac gccgagcccg cgccgggaac catggcagcg atcgagatga tcatcggctg
     5281 ctgtcaaagg cgctcggcgt tggaaggcgt tgccatgctc gcgtttgtcg aaagccagtt
     5341 gggcggcgaa ggcaaggtct ccgagaaagt ttatcgtgaa ttgaccgggc attacggctt
     5401 ctcgtcccgc gccgcggaaa cctatcggct gcacgccgcg caagacgagg gacacggcag
     5461 ccgccagatc gatgcgatcc gcttgctcgc caaggataat gcaactcaag aaagagtgcg
     5521 cgcggcggtg aagcttggcg tgacggcttt cacgctggaa tgggatggtc atgtgcaggc
     5581 gatgaccggc aaaagagaat tctggggcgg agttgtcgat ctaaacctgc ggcaaccact
     5641 cgtccgtttt aaaaatcctt aaactcactc atctaagata tccggagaag gttcgcttca
     5701 aacgactgtc tcgcgctttg cctagggggt ttgaaaaagg taagactcca tctcggcaaa
     5761 acagctcgac ctcgatcttt ctcagtcgag aagaataatt tgagattaga tcgactcgat
     5821 ccaaaagctc gtgcgattta aaaattcacg atctaaaaat cctctaccca atacctgcat
     5881 cacacgggcc agtcttctgg taattctcgc cgcaatttct gatactccgg gctgtcagct
     5941 tgttcgcggc gcaggcgagc caacgcgtca acgatttttt cttgatctat ctttagcgaa
     6001 ttggagacca tgcccgtggt ctcttcgaag ccgaggcctc aattgccgta tcagccgcgc
     6061 atgcgcgtga gaatataaag gtccttttct tttctcgtca ttgcataacc ctcaatccga
     6121 aacaccggaa aacccaactc gctgcacggt aatcgactcg gttgtcgtct gcggacgccc
     6181 ttgctgatca accgttatcc gcagtcgcac gaaaaccctc caatcatttc cctgccaatt
     6241 cacgagcaga atcaagaatg cattttcgac gactgtattc ggcggctcct ggcaagcgac
     6301 cgcttgatcg acaatgaaat ggtcctgcgc tggaatcttt accgctggcc cgaacttgcg
     6361 ttcctcagcc ctgaacttac catgcggtac ccgcacggaa agcaggctca tcggtatttc
     6421 ggtcgcgttt cgcacacgcc atttgaacag ccacaggacc gagttttcac tcctgctagg
     6481 ctcgacctgt tcaaccgcga tgctcgggtt ttcggtcttc acatcattaa aactgaagct
     6541 tctattccaa ccggcaacca aatcttgccc gacttccgtt gaacgttcga accttttgaa
     6601 cctctgagac atcttgaatg ctttgaacgg cttaaacgta ttgaagttct cggacccatt
     6661 ggcacaaccg gacttctggc acggctggaa ctgttttatt gcttcagttt cccttcagcc
     6721 cggagctcct gttgagctag cttcagaatc cgatcgttga ccacgcgatc cagagtgaag
     6781 tttttatcca ccaagccggc gcgaatgtct tcatcgagca tttgctgaag gccggctcta
     6841 gcaatcgaca aatcggaagt aaggccgggc gcgtaaagat cccaagcggc gctgacgatt
     6901 tccggctcac cctgcaaacc tgccacatag cccgccttga tcgcttcctg cttgttgaac
     6961 ttggcgagat tgatgccttg tatataggcc ttgagcatgc gcttgctggt ctcgggaaac
     7021 ttctccagga cctcattgcg ggttaccagg atattctgcg gaatacctaa cgattcgctc
     7081 atgttgctga tcaccgaata gcccgctttg atgagccgaa cgccgtcctc tgtggaaaac
     7141 ggcgccgcgg cgatgatccc cttttcgatt gccgcggcgc gcaaaaccgt gccgccgatg
     7201 gcgcgcagca ccacgtcttt gtcgggattg atattgttct ttttcaggaa ggctttgatg
     7261 gcaaactccg agtaagtccc tgcgccggta acaccgatga tcttgccttt gagatcctcc
     7321 acagtacggg tttgtttgtt ccccagcaga atataattgg tcgtgcccga agtgcatccg
     7381 acgatagcca agtccaaacc gcgcgccttg ctacgaaaaa cggcctgagc gccgccgatg
     7441 gccgcaaagt ccacttcacc ggaaaccaga gcggcaatcg ccgccggacc gccacggata
     7501 agaataggtt cgacttcaag cccttgctgc ttgaagaagc cgcgcgccag cgctgttcga
     7561 aacgggatct cgctgttcca cgccaggctg acgaaaacca tacgcacgtt cttcttttct
     7621 tgcgccaaga tcggctgcgg ctgcgctagc atggcggcca gcagcgcgcc tactgaccag
     7681 agtaaacgga aatcgtgcga gagagttcga agcttcatgc agcatccttc ccatttttaa
     7741 gatcggacgc agaacctcta aagcagttta tcttctggca tatacaaaag ttcttcctgc
     7801 cttgtcgaaa ctaatcacct cgtaaggctg cggattggct ccctccattc ccggtgaacc
     7861 caccggcatg ccggggacgg ccagacccgc cacagccggt ctttctttaa gcagccgctg
     7921 gattaaatcc gccggcacat gaccttcaat gacatagcct tccaccagag cggtatgaca
     7981 ggaatagagc ttttccggta ccttgttttt aattttgacg tctaggagtt gcttgtcgtt
     8041 gagactcttc ttttcaacct tgaaaccatt cgattgcagg tgcccgaccc acgctgaaca
     8101 gcatcctcag ccccaaccct ggtaaaccat tatggcagtg cccggcgatt ttgattgcgc
     8161 agtgcttacc gcaccgttga tcagcgggaa gagaagcata ctgacacacc agaagcgtac
     8221 aaactgtttc aaaaacattc gtcttctcct attggtttag gacgtattaa tttatcaaat
     8281 accccaggtc gggcaaagca gattttagtg actcacgttg acactgaccc agcgggcgcg
     8341 tatcggtaag gtaatccgat tctcatgagg ttggtccatg aaaaatggct ttaagattct
     8401 cgacagcgat atgcatttga tggagccggt ggatctgtgg gagcgctata tcgatgctaa
     8461 gtacaaggcc gatgcgccgc gcggcctgac cagcgacaac gttcgagatt tgcgcatggc
     8521 gcatccggat ggccgctact ggggattacc ggcaagccat aatcgcaact cctcgcacca
     8581 ccgcggccat aactttaaca aaaatcaaac catctatcgc tctcacggcg agcgcggctg
     8641 gactgccacc gtccaactcg aagcgatgga cattgaaggg atcgacgtcg cagtcctgta
     8701 tcccacccgc ggcttgcaag tcttgagcga gcctcgaatg gagccgaagt tcgccgccgc
     8761 tctggcgcgg gcctataacg attggctcta tgacttttgt aaaaccgatc cggcccgcct
     8821 tctcggcgcc ggtatgttat cgccgttcga catcgacgaa gccgtcgccg aagcgaaacg
     8881 ctgcgccacg gtgctcggtt ttcgcgccgt cttcatgcgg tcgagtatca tggaaggacg
     8941 aaattggtat gacccatatt tcgaaccgct gtgggcgacc ttggaagaat atcagatgcc
     9001 cgtgggcttt cacgaatcat cctcgtccgc ggcacggcag accggtgatt tctttgaacc
     9061 gaatttcatg ttgcggcgag ccgtcgctca accgatggag cagatgctcg ggctagtgag
     9121 cctgtgctcc ggcggcgttc tggcgcgcca tcccaacttg cgcgcggcgt ttctggaagc
     9181 gaattgcaca tggctgccat ggctgttatg gcgactggac gaaggctggg agcgcgaagg
     9241 cgatatctgg gcgcaagacc tggcggtaaa accgagcgtg tattttaaaa aacaatgctt
     9301 cgtatcggtt gagccggacg agcatggcgt caagtacgtg attgattaca tcggcaccga
     9361 tcgcctggta ttctccaccg attacccgca tggcgattcc aagtttcccg atgccgtcgg
     9421 gactttcctc gatttgccga tcagcgacga agacaagcgc aaaattctgt gggacaactg
     9481 cgcggagttc tatcgtctcg gtccatagag tttgggataa acgaatccga attcggcttt
     9541 ttcgcgccgc ccctagcttc cgcctccaac actcccggtt cgtggtttgc tggggctctt
     9601 cctggccggc cggatttgca ttatcaaaat atttgatgat caaccaaccc tgacggaagg
     9661 taagcagcga gcccgaggcg actcgctgca agaaaaaaac catgacagga tgacgatcag
     9721 gtcatcaccg aagtgctttt cacgtgtgaa aggtcattaa gcggcggtcc gatccggggg
     9781 agggttagtg gctgagcagg tattttcttc gactgaactt tacgacgcct ggccggtcct
     9841 atcggtcgaa gacaaactag aaggatttaa gctcttgccg ccggaggagc gcgagctttt
     9901 tttcaagcaa ttgagcgccc gggacgaggc caacttgctt gaagcgtttc cgccggggga
     9961 gcgccgcttc tggattcgtc tcttagctcc ggaagatgca gcggatctta ttcaagaagc
    10021 agcggccgat gaacgggaaa agatggtttc tataatcgat gaaccgttgc ggagcgaaat
    10081 caaagggctg ctggcatacg cagaagacca ggccggcggt ttgatgagcc cgcgctacgc
    10141 ccgcctgcgg cccaatatga ctgtcgatga agcgttcgcc tatcttaggc gcgatgcgcg
    10201 ggatcgcgag aaaaaattct attacgcatt cgttgtcgac ccgcaagaaa gactccttgg
    10261 cgtcgtctca tttagggagc tcttctcggc gcgcggcgat cagtcgattc agaacgtcat
    10321 gcgtacggat atcgtcacgg cgcgcgacaa tatggatcaa gaagcgctta gtgatctttt
    10381 tgccaggcat aatttgatga tgataccgat tgtcgatgct ggaacccata tcaaaggaat
    10441 cgtgactcac gacgacat
//
LOCUS       DASUVS010000395         5773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1703208, whole genome shotgun sequence.
ACCESSION   DASUVS010000395 DASUVS010000000
VERSION     DASUVS010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703208"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            457..3696
                     /locus_tag="VF182_21155"
     CDS             457..3696
                     /locus_tag="VF182_21155"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6439639.1"
                     /translation="MQVVPFYRSTHARLILLVLVATVPFIALNLHLAKEERSHYRSAA
                     LDQSLSFARLVSSRVDDHISNMESLLHAISAVVSNDLAAIASNDARLTQIQSGLPSYF
                     HSISVLTPDGRMLNSSTASPEERKNIRVTDRKYFKEAVALGLAIGEPVVSRTSENWVL
                     ILARSILGPDGSLRAVVSMSTRLTSFQQLLVPERLPAGSVMTLVNEQGIVLARSSEPE
                     KWVGRNFSGSDNFQRAAVEREFSAETVAFDGITRLSSYTTARRAPWIIYVGIPTETAL
                     APTRAAMWRFAALAALTSILALALAWWLSKMTADPLRQLSRDVAQLAFGNLSHRSTVK
                     SSSEVGLLANEFNRMAGELQNERTRLQISEERLRLAAAGARLGTWHWNLSTGELIWSE
                     RCREIFGVSADAPVSYDQFLALVHPEDRERLKQLVTGALREKTDFEFEYRIIPGSNTI
                     RWIVALGRGEYDVADNPSRMEGVVLDITERKKAESAILKERDFSEAILDSLPGVFYCY
                     DREFHFRRWNKNLERVTGYTGSEIAGMSPLDFFVGPGRELIQKRIEEVFATGRSQAEA
                     EFIAKNGTRIPYYFTGLTIELDGTPHLLGVGIDLSMRKQAEAERDRLFNLSLDPLCVA
                     GFDGCFKQINPAWTQTLGWPANELLTRPWLDYVHPEDREATQQAGASLLAGQPVLGFE
                     NRYRHQNGSYRWLAWNSFPLSEVGLIFSVVRDISERKRTDEELRKSEAELRAAQERAK
                     LGSWEVDLSTRTVSWSAEMFRLLNCDPARGTPPPEQFLEMVHPDDRHLIEDRLTRVVQ
                     TGDQFTQEFRSDPSRGPLQYFNAIGQATKNAEGQVVHLVGTIQDITDLMKARRALDSA
                     EREAALGRMAASIAHEINNPLFAIKTRLYTLKKTVADRPETTEKLDLVMGQLDRIGRA
                     TRSMLGFFKQRAAHSTSVSYADVIRAATDLFEASFSAKGVRLVVSLPQSLPAVTVSVD
                     ELQEVLINLLENERDALKNDNDVYVSAQTNDHQIIISVEDNGPGLGADPEALFAPFYT
                     TKSTGTGLGLTIARRICESYGGKLTAQNRKEGGARFEIVLPLSTKREET"
     gene            3693..5021
                     /locus_tag="VF182_21160"
     CDS             3693..5021
                     /locus_tag="VF182_21160"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6439640.1"
                     /translation="MRTLVVEDDVAVVDALRDSFQNSSLNIDYATDGESALKKIPDVD
                     LLVTDLRLPGMDGTELLNEARRRKPELEVVIMTAYGSIPSAVEAMRRGARAYLTKPFD
                     PEELIIHIRTVEETLKLREMAFRFSRGELIGSSLLMQKLYQEIDLAASSNAPVLITGE
                     TGTGKELAARAIHDLSQRKNGPFIAVNMGALPKDLIESELFGREKGAFTGADARKRGR
                     FTLAEGGTLFLDELTSLSLELQPKLLRAIETKQIWSLGAEKPEHVDARVVAATNADVE
                     AMIKASAFREDLYYRLNIHRIFMPALRDHPEDIPTITRMLLERANVEDTNGSRRPTEI
                     SGETLAALVSRPWPGNVRELANVLERAALRSKTSGASRIEVEHLDPEPGSFPLLSFKE
                     AKDKAAEEWSKRAIRTALALTEGNVTKAAALLKMNQNALFRLIKKYGLKS"
     gene            5438..>5773
                     /locus_tag="VF182_21165"
     CDS             5438..>5773
                     /locus_tag="VF182_21165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6439641.1"
                     /translation="MDREIKEANSKTILIVEDDNVARRGLKRFLQVEGWKTLEAPNGV
                     QGLRVFRSHLPEIILTDIVMPGFDGFFLTAVIKSISPQSPVIIMTGLGDADFDGKAQH
                     LGVSALLRKP"
BASE COUNT         1353 a         1564 c         1592 g         1264 t
ORIGIN      
        1 aaccggcgta cggttagatt tttctctgat atcggcttca cgggtatgaa cagaaatgta
       61 gcagatctgg aattacaccg aatgaaatag aaacgtcaag ctcctcgaaa gcgactccgc
      121 gatatatcgg cttcatcaaa agttcgactc ttattctgac aatgagcgca ccagcgcggc
      181 taggccgcgg gccgcggaaa cttcataaaa actgtaggag cgagctcgac gatgttgttc
      241 tctccgcatc atccatcccc tggcgtcata aacaaaaatt gacacgggat ctgaggttcg
      301 agtagtgtct tcgctaggaa gtcaggccgg ctcttcttca acctgatgat ctaacaaagg
      361 ccgcgttcag agcagattgg tcctcgcgta gccaagcaag cttcagctac tgggctgtga
      421 agctcatcac gcgctcagcc ggcggagaca gcgacgatgc aagtggttcc tttctaccgt
      481 agtacacacg cccgactcat cctcttggtc ctggtcgcga cagtgccgtt catagctctc
      541 aacttgcatc ttgctaaaga agagagaagc cattaccgca gcgctgctct tgatcaatcc
      601 ctttcctttg cccggctggt aagctcgcgt gtcgatgatc atatcagcaa tatggagtct
      661 ctccttcacg ccatttccgc ggtcgtgtct aacgatctag ccgcgatcgc atccaatgac
      721 gcgcgactca cgcagattca gtccgggctc ccaagttatt ttcacagcat ctcggtgctt
      781 acgcctgatg gtcgcatgtt gaattcgtcg acggcctctc ccgaagaacg aaaaaacatc
      841 agagtgaccg accgcaaata tttcaaagaa gccgtggcgc tcggcttggc gatcggggaa
      901 cccgtcgttt cgcgcacatc ggagaactgg gttctgatct tggcccgaag cattctcggt
      961 ccggacggtt ccctaagggc cgtcgtctcg atgtcaaccc ggttgacgag cttccaacaa
     1021 ctgctggtac ctgagcggtt acccgccgga tcggtgatga cattagtcaa cgagcaggga
     1081 attgttttgg ctcgctcctc ggagccggaa aaatgggtcg ggcgcaattt cagcgggagc
     1141 gataattttc aacgggccgc agtcgagcgc gagtttagcg cggaaaccgt cgcgttcgat
     1201 ggcattaccc ggctgtcttc gtatacgacg gcgcggcggg caccgtggat tatctatgtg
     1261 ggtatcccca cggaaactgc gctggcgcca acccgcgccg ccatgtggcg atttgccgcc
     1321 ttagccgcgc tcacttcgat tctagcgtta gcgctcgcct ggtggctgtc caaaatgact
     1381 gccgatccgc tacggcaatt gagccgcgat gtggcgcagc tcgcttttgg caatctgtcg
     1441 catcgatcca cggttaaatc gagtagcgag gtggggctgc tggctaacga attcaaccgc
     1501 atggccggcg agctacaaaa cgagcgcaca cgcttgcaga tcagcgaaga aaggctgcgg
     1561 ctggcggcag caggcgctcg actcggcacc tggcattgga atcttagcac aggcgaactc
     1621 atctggtcag agagatgccg cgagatcttc ggcgtgtcgg cggatgctcc ggtgagctac
     1681 gatcagtttc tagcgctggt tcatcccgaa gaccgcgaac ggttgaagca gctagtcacc
     1741 ggtgcgctcc gagaaaaaac ggatttcgaa tttgagtacc ggatcattcc tggcagtaac
     1801 accattcgat ggatcgtggc tctcgggcgt ggcgagtatg atgtcgcgga caatccctcg
     1861 cgtatggaag gggtggtttt agacatcacc gagcgaaaaa aggccgagtc ggctatcctg
     1921 aaagaacgcg atttttctga ggccatttta gacagcctgc ccggggtttt ctattgctac
     1981 gatcgcgagt ttcatttccg gcgctggaac aaaaacctcg agcgggtgac cgggtatacg
     2041 ggcagcgaga ttgccggcat gagcccgctg gatttttttg tcggccctgg gcgggagttg
     2101 attcagaaaa ggattgagga ggtctttgcg accggccgat cgcaggccga agcggaattc
     2161 atcgccaaga atggaacgcg catcccctac tattttacgg gtctcaccat cgagcttgac
     2221 ggtacgcctc atttactagg agtgggaata gacctttcga tgcggaagca ggccgaggcg
     2281 gaacgcgatc gtttattcaa cctgtcgctc gatccgcttt gtgtggctgg cttcgacggc
     2341 tgctttaaac aaatcaatcc cgcatggacc cagacactag gctggcccgc gaacgagtta
     2401 ttgaccagac cttggctgga ttacgtccat cccgaggatc gcgaagccac acaacaggct
     2461 ggagcttcgc tactggctgg acagccggtg ttaggttttg aaaaccgcta tcggcatcaa
     2521 aacggttctt atcgctggtt ggcatggaat tctttcccgc tttccgaagt cggtttgatt
     2581 ttttccgtcg tacgcgatat cagcgagcgc aaacgcaccg acgaagagct gcgcaaaagt
     2641 gaagcagagc tccgcgccgc gcaagagcgc gccaagcttg gcagctggga agtggaccta
     2701 tccacccgaa ctgtctcatg gtcggcggag atgttccgat tgctcaactg tgacccggcg
     2761 cgcggaaccc cgccgcccga gcaatttctc gagatggtac atcccgatga ccgtcatctt
     2821 atcgaggacc ggctcacgcg agttgtgcaa acaggcgatc agttcactca agagtttcgc
     2881 agtgatcctt cccgcggccc cttgcagtac ttcaatgcaa tcggccaagc aacaaaaaat
     2941 gccgagggcc aggttgttca tttagttggg acgattcaag atatcactga ccttatgaaa
     3001 gccaggcggg ccttggacag cgccgaacga gaagcggcgc tcggccggat ggccgcctcg
     3061 atcgcacacg aaatcaacaa tcctctcttc gcgatcaaga cccgactata taccttaaag
     3121 aagacggtcg ccgaccggcc tgagacaact gaaaagctgg atcttgtgat gggtcaattg
     3181 gatcggatcg gtcgtgccac gcgatcgatg ctcggctttt ttaagcaacg agctgctcat
     3241 tcgacgtccg tttcctatgc cgacgtgata cgggccgcca ccgatctctt cgaagcaagc
     3301 ttttcagcca agggagtgcg gctcgtcgta agccttcccc agtctctgcc cgccgttacg
     3361 gtcagcgtcg atgaattgca ggaagttctc atcaaccttc tcgagaatga acgcgacgct
     3421 ctcaagaacg acaacgacgt ctatgtttcg gcgcaaacaa acgaccatca gatcatcatc
     3481 agcgtcgagg acaacgggcc tggattagga gcggatcccg aggctttgtt cgcgccgttc
     3541 tataccacca agagcactgg tacagggttg ggacttacga ttgcaagaag gatatgcgaa
     3601 agctacgggg gaaagctcac cgcgcaaaac aggaaagagg gcggagcgcg gttcgagatc
     3661 gtactcccac tttccaccaa gcgtgaggag acatgagaac gttggttgtc gaagatgacg
     3721 ttgcggtagt ggatgctcta cgagattcgt ttcaaaattc ctcgttgaat atcgactatg
     3781 cgaccgatgg agaatctgcg ctaaaaaaga tccccgatgt agacttgttg gtgaccgacc
     3841 tgcgcttgcc tggaatggac ggcacggagc tgctcaatga agcgcggcgc cgcaagccgg
     3901 agttggaagt tgtcattatg acggcctacg gaagtatccc gtcggcggtt gaggcgatgc
     3961 ggcggggagc ccgagcttat ctgacaaaac cttttgatcc ggaagagctc attattcaca
     4021 tccgaaccgt tgaagaaacc ttgaagctcc gcgaaatggc ctttcgtttt tcccgcggtg
     4081 agctcatcgg ctccagcctt ttgatgcaga agctgtatca ggaaatcgat ctggcggcga
     4141 gctccaatgc tccggttttg attacgggag agaccggcac cggaaaagag ctagccgcac
     4201 gcgcgattca cgacctctcg cagcgaaaga acggaccgtt catcgccgtc aatatgggag
     4261 ctttgcccaa ggacctcatc gaatccgagc ttttcggccg tgaaaaggga gccttcacag
     4321 gcgctgacgc gagaaaacga ggacgtttta cgctcgccga aggcggaact ttatttttgg
     4381 atgagctcac atcgctatcc ctcgagttgc agccgaaact gctgcgcgct atcgaaacca
     4441 agcagatttg gagtcttggc gcggaaaagc cggagcacgt cgacgcccgg gtagtagctg
     4501 ccactaatgc agatgtcgaa gcgatgatta aggcttcagc atttcgtgaa gatctctact
     4561 acagactgaa tatccaccgt atattcatgc cggcgttaag agaccatccg gaggacatcc
     4621 ccactattac ccgcatgctc ctcgaacgag ccaacgtcga ggacacgaac ggtagccgca
     4681 gacccacaga aatctcgggt gaaacgctgg ctgctttggt gagtcgcccc tggcccggaa
     4741 acgtccgaga actggcgaat gtactggaaa gagcagcgct gcgcagcaaa acctcgggcg
     4801 catcaaggat tgaggttgag catctcgatc cggagcccgg aagttttcct ttattgtcgt
     4861 tcaaagaggc gaaagataag gcggccgaag aatggtcgaa gcgggcgatc cgaactgctc
     4921 tcgccttgac cgaaggcaac gtcacgaaag cagcggctct gttgaagatg aatcagaacg
     4981 ctctgttccg gctcatcaag aaatatggcc taaagtccta agacactcgg gctcgcttcg
     5041 ctcaagtttc gtagccgttt gttcagccat tcactgatac ccgctccaga accgatcccc
     5101 gaaaacgcag cacattgttc atccagaaga ccgctcttat tgcatttgga gtccggactc
     5161 gctggaactt gactcgatgt ttcccgaatc gataaggatc tcctcacgct tgctattcca
     5221 aaagctaaga acacaatttc gccaactcgg ctgcaatatc atcgcgctgc tgtcgccgtt
     5281 cggcgatagc caaattcctt tctgtttcct gcgttaatca caactatcct tccacaggta
     5341 tagccgcgcg gcgatagccg cacggcgcag tatcgaattt gcctggcaaa aaattactcg
     5401 cgaagactgg cacctgggtt gctcaagaga gcccaccatg gacagagaaa taaaagaagc
     5461 caattcaaaa acaatcttaa tcgttgaaga tgacaatgtg gcccgccgtg ggctcaagag
     5521 gtttctccag gtggaaggat ggaagacttt agaggctccg aatggggttc aaggcttgcg
     5581 ggtgtttcgc agtcatcttc cggagatcat tttaacggat attgttatgc cgggcttcga
     5641 cggctttttc ctgaccgcgg tgatcaaatc gatcagcccg cagtcgccgg tgattattat
     5701 gactggactc ggcgatgccg atttcgacgg aaaagcgcaa cacctagggg tcagcgcatt
     5761 gctacgcaaa cca
//
LOCUS       DASUVS010000396         6560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1267296, whole genome shotgun sequence.
ACCESSION   DASUVS010000396 DASUVS010000000
VERSION     DASUVS010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6560
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267296"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..395)
                     /locus_tag="VF182_21170"
     CDS             complement(177..395)
                     /locus_tag="VF182_21170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439642.1"
                     /translation="MANLFYNDRLIIAYAALQQSSKLWSAGAEITWKIDGQRQSHTIE
                     ALPDRFKTAEEAERFVINFAKSWIDANP"
     gene            complement(486..935)
                     /locus_tag="VF182_21175"
     CDS             complement(486..935)
                     /locus_tag="VF182_21175"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6439643.1"
                     /translation="MPKVASLGHLGLYVRDIERSKTFYRDIVGLKVSDENPKTGSAFM
                     TAKDRLDEHHELLLIPGREDGKVVQQISFRCSSLTDVKEFYRLFLERKVPIIRTVSHG
                     NAVGLYFEDPDGNQLEVYWPTGIDWPQPFGKAIDLSASEEEIIANLT"
     gene            complement(1024..1158)
                     /locus_tag="VF182_21180"
     CDS             complement(1024..1158)
                     /locus_tag="VF182_21180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439644.1"
                     /translation="MSQSDWAKTYATMDKRIILKYEQLPRGEQIGKAPQAGKQDKRKS
                     "
     gene            complement(1181..1942)
                     /locus_tag="VF182_21185"
     CDS             complement(1181..1942)
                     /locus_tag="VF182_21185"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439645.1"
                     /translation="MVAWRARIGLIKPTHRGKSFAYWYNNAPEGVEIVPTFIGFRSEK
                     RESFLEGFKKAEELAVQLKDVGCDIIAVSGTPPFLLKGLDFERQWATDLSAKIGLPVV
                     TPMEPHALALQALGARTVAIATYYGQELNQAIVDYFSRFGIESELMPGLSATGEGAGL
                     YTTSLRALDEVSHTDVYRHCRKALQGMPPVDALYINGGGWDAAPAIDFIERDLKIKVV
                     WALAAEMWFTYRALKIDNPVMRGGMLLRDNYAPRV"
     gene            complement(2547..3890)
                     /locus_tag="VF182_21190"
     CDS             complement(2547..3890)
                     /locus_tag="VF182_21190"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX6439646.1"
                     /translation="MQPRTSAVMGKRISAAVVEFIKVWGLLALVVLVGFIVTYQYVGA
                     PPPKVVRIATGAKNGAYYTFAQEYARLLASDGITLEVVPSAGSVENLELLKKGEVSLA
                     LIQGGSVTNEDKERLQSLGSLFLEPVWIFTRKQSGIKRFLELKGKRLSVGVAGSGTQL
                     LTTQLLSAAGLTESNTRLIREETAQAIESLSKGKIDAAFFVASPEAPIIHKLFAAPAV
                     ELLNFDRAAAYGRLFPFLTPVILSEGVINLEQNIPFRDTTLVAASANLAARGDLNPSL
                     IPAILNAVTRVHQTGGLLEHKRQFPSVDFVDLPLSEDARRYLINGPSFLFRWLPYGTA
                     VLLDRLKILVLPFLALLIPLFKIAPPLYTWRIRSKIYRWYATVRELESSIQEDKVSGD
                     AAALINRLSELDHQVASVSVPLAYAAELYHLRLHIRFLQEKVQSSASADPTPMSN"
     gene            4115..5476
                     /locus_tag="VF182_21195"
     CDS             4115..5476
                     /locus_tag="VF182_21195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439647.1"
                     /translation="MAPEVKITLGRLPRFWDRNVLFFCNMHSIFYDNQEEVNDLISQI
                     YGAHSYGSRVVSILNLLFRRGPNKIFLEAPPEPNLLDYLSGDLRLSLPSFAVLDHRGY
                     QDLVRSLNTGGSEVEPQVEDLKSHAATWVDGFVTDRLLIEVATRLNKRTISTFEGSKN
                     GNNKYLLYRHQLERGLPVFETLLAANHREVPSALDRLRALGYSKAVVKAQIGASGCGM
                     IKLDTGSPRLDSMPGYLFFEGPSMVQGWLDEEMPSIRRIGSPSIQMFLDDDVVSLFDW
                     TEQILSEASVHEGNMSPPPYIQKYPELTEELFRQGSIAGEWLHAQGYRGTASIDFVVI
                     EQDGLNQVIICEINARVTGATYPAVLARHFIPDGCWKMRNIQFRKSLDGDQLLSLVDH
                     AKVLYRPEKDTGIIPFNFNTDQQGKVIKGQFLCLGEDQEQCSEVLGRACTALPVEWGY
                     DRD"
     gene            5463..>6560
                     /locus_tag="VF182_21200"
     CDS             5463..>6560
                     /locus_tag="VF182_21200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX6439648.1"
                     /translation="MIETSRSDESLRRRLIELTRDLIIIPSTRDRPEDIERCMEFVIN
                     HVELPDAVEVRRFNEKGSPSTVILPHNVSAPEVLLLAHLDVVARPEGAEYRSLVEDGR
                     IYGPGSGDMKGELAILLEIFRDFHERLPGVSLGLAVTSDEENGGHHGTRFLFEKAGLR
                     CGVAIIPDSGSLNEIAVEEKGILHLKLKAHGSSGHASRPWLAENPLEKLISKLNEVRN
                     YFETLKNGDGHWHPTIAVTVMQTENRVSNRIPSLAEAVCDVRFPPPYKSKEVISQLER
                     FMDPKMALEVLVSAEPTKVSPDPLFLAATQEITQKPVTLIREHGGSDARFICGYGIPV
                     IMSRPYVGNLHAENEWIDIASMETLYHIYERY"
BASE COUNT         1568 a         1790 c         1670 g         1532 t
ORIGIN      
        1 ggaaggcgca accaccggcg tcactccttg cacaaactgc gcgaatcctg cgacatcgta
       61 tgcgaatgtg acgaagaatt tcttcgacta cgcagcggaa tggatcaatg accggttttc
      121 atctaactga ttcgcgacaa cgtttgcaga gccatttatg acggacggct ggcaagctac
      181 ggattggcgt caatccagct tttagcaaag ttgatcacaa accgttctgc ctcttcagcg
      241 gttttgaatc gatcgggcaa cgcttcaatc gtgtgcgatt gccgttggcc atcgatcttc
      301 caagtgattt ctgcgcccgc gctccagagc ttgctgcttt gctgaagagc ggcgtatgca
      361 atgatgagcc gatcgttata aaatagattt gccatgatcg tgggctatag agatccgagg
      421 agaagtcctc cacgccttgc actgcatctt gaattgctca taagtcgagc tatgccgtcc
      481 tgcgttcacg tcaggtttgc gatgatctcc tcttcgcttg ccgacaagtc tatagctttg
      541 ccgaagggtt ggggccaatc gatgcctgtg ggccaataaa cttcgagttg attgccgtcc
      601 gggtcttcga agtacaggcc cacggcattg ccgtggctga cggtgcgaat aatcggcacc
      661 ttgcgctcga gaaaaagacg gtagaattct ttgacgtccg tgaggctgga acaccggaaa
      721 gaaatttgct gcaccacttt gccatcctca cggcctggaa tcagcagcaa ctcgtgatgc
      781 tcatcgagac ggtccttagc ggtcatgaat gcgctgccgg ttttcggatt ctcatcgctg
      841 accttgagcc caacgatatc gcggtagaat gtcttcgagc gctcgatatc gcgcacgtac
      901 aaacccagat gacccaaaga agccacttta ggcatattgc ctccgttcaa aaatttttga
      961 ccggatccgt aattttgttc cttaagcccg gcgacattcg aattcagaat gcacggccgc
     1021 caatcaggat tttcttttgt cctgttttcc tgcctgcggc gccttgccga tctgctcgcc
     1081 tcgtggcaac tgctcgtatt tcaagatgat ccgtttgtcc attgtcgcat atgtttttgc
     1141 ccaatcgctt tggctcataa aacctccaca aggaacttct ttaaacccta ggcgcatagt
     1201 tgtcgcgcag caacatgccg ccgcgcatta ccggattatc gatcttcagc gcgcgatagg
     1261 tgaaccacat ttcggccgcc aaggcccaga cgactttaat ctttaaatca cgctcgataa
     1321 agtcgatcgc cggagccgcg tcccacccgc ccccattgat gtagagggcg tccaccgggg
     1381 gcatgccctg caacgccttt cggcagtgcc ggtagacgtc ggtatgagat acttcatcca
     1441 gagcccgcag cgaggtggtg taaagtccgg cgccctcgcc ggtcgcactg agtcccggca
     1501 tcagctctga ttcgatgccg aagcgcgaaa agtaatcaac gatcgcctga ttgagctcct
     1561 ggccatagta ggtggcaatc gctacagtgc gcgcgccgag agcctgtagg gcaagcgcat
     1621 gcggctccat cggtgtgact accggcagac ctatcttggc cgagagatcc gtcgcccact
     1681 gccgctcaaa atccaagcct ttgagcagaa acggcggcgt cccgctaact gcgatgatat
     1741 cgcaaccgac gtccttcagc tgaactgcga gctcttcggc ttttttaaat ccttcaagaa
     1801 aactctcgcg cttctcgctg cgaaagccga taaacgtcgg cacgatctcg acgccttccg
     1861 gcgcgttgtt gtaccaatag gcgaatgact taccgcgatg cgttggcttg atcagaccga
     1921 tgcgcgcgcg ccatgcaacc atctaaatct cctcctattc ggcttactta tcgtcaacga
     1981 tcttaattgc ggctatcacc ggcagcggcc ggtgtcaaag acgattaggc gcgaacacat
     2041 ccgtcattat cctgcataag ctgagaaaaa cgtttgcgcg cccgcgctat ggtcaagacc
     2101 ctctaagact caaagcgctg cctcatggta tgacaacatt cctcctcatg acaaatcaga
     2161 cagttgtcgc aaagactcac accgcagaaa ggacaattaa actcctcacc atgaagaatt
     2221 tcgttccggc attcgccgca ttcgatcgtt ccattgatca gctgaaacat tagctgttcg
     2281 ccgctcgcgt tctaatgggt tccataccgg ctcacccatc cacgcggctt atcatggttg
     2341 cgccaactgg agcaatgaaa aaccggctga caattagaga gtagccggtc aacatcttca
     2401 aagttttgtt gaaggttatc gaggctgcca ttgccgccgt gcgcgattca aacctgcaca
     2461 tgacaagggc aaaaggcggt aagcattgga tcgctctaga gatgcaatgc cttagtcgct
     2521 taccgatcct aaagagcacc cgtaagctaa ttgctcatcg gcgtcggatc ggccgatgcg
     2581 ctactttgca ctttttcctg gagaaagcgt atatgcagcc ggaggtggta taactccgcg
     2641 gcataagcga gaggaacgga aaccgaagcc acttggtggt caagctcgct taatcgattg
     2701 atcagtgctg ccgcgtcgcc ggaaacttta tcttcttgga tcgacgattc gagttcccgc
     2761 acggtcgcgt accaccgata aattttcgac cgaattcgcc aggtataaag cggcggagcg
     2821 attttaaaca acggaatcag caaagcgaga aaaggcaaga caagtatttt gaggcggtcc
     2881 agaagcacgg ccgtgccata gggcaaccat ctaaacagaa aagaggggcc gttgattaga
     2941 taacgacggg catcttcact tagaggcagg tcaacgaaat ccaccgacgg aaactgccgt
     3001 ttatgttcca gcagcccgcc tgtttgatgc acccgggtga cggcatttag gatagccgga
     3061 atgaggctag gattgagatc tccacgagcc gccagattag ccgaagccgc taccagggtt
     3121 gtatcacgaa aggggatgtt ttgctctaga ttgatcactc cctcgctaag aatcaccgga
     3181 gtcaaaaacg ggaacaagcg gccgtatgca gccgcgcgat caaaatttag aagttccacg
     3241 gcgggcgccg caaagagctt atgaatgatt ggcgcctcag gcgaggcgac aaaaaacgcg
     3301 gcatcgatct tccccttcga taacgattct attgcctgcg cggtctcctc tcgaatgagc
     3361 cgcgtattgg attccgtaag cccggcagca gataaaagtt gggttgtcag caactgagtg
     3421 ccgcttccag caacgcctac tgacaaacgc ttacccttta attccagaaa tcgtttgatc
     3481 ccgctctgct tgcgcgtaaa aatccacacc ggctcgagaa acaagctgcc cagagactgg
     3541 agacgttctt tatcctcgtt agtcacgctg cctccctgga taagcgccag cgacacttct
     3601 cccttcttca gcaattccag gttctctacg gatcctgcgc tcggtaccac ctcgagtgtg
     3661 atgccgtcgc tggccagcaa acgcgcatat tcctgggcga aggtgtagta cgccccgttc
     3721 ttcgctcccg ttgcaattcg aacaactttc ggcggcggtg cgccgacata ctgataagta
     3781 acgatgaacc ctaccagaac aactaatgcg agtaggcccc ataccttaat gaactccaca
     3841 acggctgctg atatgcgttt acccatcact gccgacgttc ttggttgcat gcgcatcgcc
     3901 gcttcggcgg cctgaccttt ggccaagcga ctatgattct aaggcggcaa agctatcaag
     3961 tctacataat cctctgccac tatctatcgg aatcaaagct ctccaacagc aggcctcaac
     4021 gatctattag cagcgggcga acacgccgcg agatacgacc gcgctgcgga tggatccatg
     4081 tgcattaata atcgagcgtg ctagtctaag gcacatggcg cccgaagtta agattacgct
     4141 gggtcggttg ccgcgattct gggaccgtaa cgttctgttc ttctgcaaca tgcattcgat
     4201 tttttacgat aatcaggaag aagttaacga tctcatcagt caaatttacg gcgcccattc
     4261 ctacggcagc agagtcgttt ccatcttgaa cttgctgttt cgacgcgggc ctaacaagat
     4321 ctttttagaa gcgccccccg aacccaatct gctggattat ctttcgggtg atctgcgatt
     4381 gtctctgcct agttttgccg tgttggatca ccgcggctat caagatctcg tgagaagtct
     4441 caacacggga ggttcggaag tagagccgca ggtcgaagat ttgaaatctc atgcggcaac
     4501 atgggtggat ggttttgtta cggaccggct tctcatcgaa gttgcgaccc ggttgaacaa
     4561 acggaccata agtacgtttg aaggcagcaa aaacggcaac aacaaatatc ttctttatcg
     4621 ccatcaattg gaacggggat tgccggtctt cgaaacactt ctcgcggcaa atcaccgaga
     4681 agtgccatct gctctcgatc gcctgcgagc gttgggctat tcaaaggcag tggttaaagc
     4741 ccagatcggt gcttccggct gcgggatgat caagctcgat accgggagcc cgcggctcga
     4801 tagcatgccc ggctacctat tctttgaagg cccttctatg gtccaaggct ggctcgatga
     4861 agaaatgccc agcatccgcc gcatcggctc tcccagtatt cagatgtttt tagatgatga
     4921 cgttgtttct ttattcgact ggaccgagca gattctgagc gaggcaagcg ttcatgaagg
     4981 caacatgtcg ccgcctccct atattcaaaa atatcctgag ctcacggaag agcttttccg
     5041 acaaggctca atcgccggcg aatggctgca cgcacagggt tatcgcggca ctgccagtat
     5101 cgactttgtc gtcatagaac aggacgggct taatcaggtg atcatttgcg agatcaatgc
     5161 acgggtgaca ggcgcaacct atcccgcagt actggcgcga cactttatac ccgacggatg
     5221 ttggaagatg cgaaatattc aatttcgtaa atcacttgac ggtgatcagc ttctctcgct
     5281 tgtcgatcac gcaaaagtgc tctatcggcc ggagaaggac acaggaatca ttccttttaa
     5341 ctttaacacc gatcagcaag gtaaagtcat caaaggtcag tttctctgcc ttggagaaga
     5401 tcaggaacaa tgcagcgagg tattggggag agcctgcacc gcattgccgg tcgaatgggg
     5461 ttatgatcga gactagccgt agcgatgaat ctctgcgccg gcggttaatt gagctcactc
     5521 gcgatctgat catcattccg agcacgcgcg atcgtccgga ggatatcgag cgttgcatgg
     5581 aattcgtcat caatcatgtg gaactccccg atgcagtcga ggttcgccgc tttaatgaaa
     5641 agggtagtcc ctctacggtg atcctgccgc acaatgtttc agcgcctgag gttctgcttc
     5701 ttgcgcacct cgacgttgtt gcacggcctg aaggcgcaga gtaccgctcg ttagttgagg
     5761 acggcagaat ttatggtccc ggcagcggcg acatgaaagg cgagttagcg atactgctgg
     5821 aaatctttcg tgacttccat gagcgcttgc ccggagtttc actcggcctc gcggtaacat
     5881 ccgatgagga aaatggcggg catcatggca cgcgctttct tttcgaaaaa gccgggctcc
     5941 ggtgcggcgt tgccatcata cccgactccg gctctctcaa tgaaatagcc gtcgaagaaa
     6001 agggcattct tcacttgaag ctcaaggcgc atggatcctc aggccatgct tcgcggccgt
     6061 ggctcgctga aaatccttta gagaaattaa tatccaagtt gaacgaggtg cgaaactatt
     6121 tcgaaactct caagaacgga gacggacatt ggcatccgac cattgcagtc actgtgatgc
     6181 agactgagaa tcgagtatcg aaccgcattc catcgctcgc agaagcggtc tgcgatgttc
     6241 gattcccgcc tccgtataag tcgaaagaag tgatttctca gttagaacga ttcatggatc
     6301 ctaaaatggc gctcgaagtg ctcgtcagcg ccgagccaac taaggtgtct ccggatccgc
     6361 tctttcttgc cgcgacccaa gaaatcacgc aaaaaccggt aacactgatc cgcgagcacg
     6421 gcggaagcga tgcccgcttc atttgcggct acggaatacc ggtgatcatg tccagaccct
     6481 atgtcggcaa tctgcacgcc gagaacgaat ggatcgacat cgcttcaatg gaaacactct
     6541 accacatcta cgaacgttat
//
LOCUS       DASUVS010000397        33797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_829753, whole genome shotgun sequence.
ACCESSION   DASUVS010000397 DASUVS010000000
VERSION     DASUVS010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 33797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33797
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829753"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1053..1280)
                     /locus_tag="VF182_21205"
     CDS             complement(1053..1280)
                     /locus_tag="VF182_21205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012824291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439649.1"
                     /translation="MKKIKRRLLILAIAAAMIAPAGCKRSEDGEGSAEKAGKKIDNAL
                     EKAGQETGKLMERVGESMQKTGEKMQDTSKK"
     gene            complement(1502..2710)
                     /gene="ilvA"
                     /locus_tag="VF182_21210"
     CDS             complement(1502..2710)
                     /gene="ilvA"
                     /locus_tag="VF182_21210"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX6439650.1"
                     /translation="MLSFVDVEEARRRIRDQVYLSPLSYSETISRMTSNKVYFKLDNL
                     QMTGSFKERGALNRLLSLTQDEAERGVIAASAGNHGMAVAFHSRRLNVESTIVMPLHA
                     PLIKVGRVRLYGAHTLLYGEDYDGAFTEAQRLSQARGLTLLHAFNDPTIIAGQGTIAL
                     ELYEQNPHLDAIIVPVGGGGLIAGIALVLKVLNPHIRIIGVQAEAIPSMKAALEKGAP
                     ISLEPATTIADGIAVRKVGETALDLVEKHVDEIVTVTEGEIANAVLLLLEIEKTVAEG
                     AAAAPLAALINKKVNLIGKNVALIVSGGNIDVNLISRIIEKGLIQDGRLSRFSVVIRD
                     RPGNLARLSQRLADLDANILQIGQSRGFGRIAIDQTEVELVIETGGLDHVQRIRSELS
                     KDGFQVRWES"
     gene            2881..4005
                     /locus_tag="VF182_21215"
     CDS             2881..4005
                     /locus_tag="VF182_21215"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX6439651.1"
                     /translation="MPTQENRAADVARYRQNYLVEMDGIELYRALAAAEKDERRAVIF
                     RKMAQTEERHARRWVRLIETAGFTVPDYRPTVRVRVLAWMAKHFGTHRVIPIISSLEA
                     SDEPGYLNQPEAVGLPAQERAHSRALRAMDDGISTQDAIIGSERWHVMSRGGSLRAAV
                     FGINDGLVSNFSLVMGFAGADARPEYIVLAGVAGLLAGSFSMAAGEYVSMRAQRELFE
                     QQIAMEQQELEMSPKEEEEELSLIYQAKGIPEREAAMLARRIIANPKTAIDTLAREEL
                     GLNPAELGSPWSAALSSFLAFILGAMIPVLPYLFSTGQLAWFVSGLLSCLALLGVGAI
                     ISIFTAKGPWISGLRMLGIGLLASAITYGVGWLFGVAVPG"
     gene            complement(4062..5507)
                     /locus_tag="VF182_21220"
     CDS             complement(4062..5507)
                     /locus_tag="VF182_21220"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEX6439652.1"
                     /translation="MTRKSRFSLTCVIAALLVVGGWVVISARAAQTQPASPKYVFLFL
                     ADGGGIAHLEITRQYNRQVHNEGMVISDKIMTEGSVGLMTTHAANSLSTDSAAAATAL
                     ASGCKAKIGALGVCADGSMPDTVMEYARRRGMRIGLVTNSTVYDASPAAFVCHVPNRR
                     HFASIIDRYIELEPDVILGGGKEFFIPKSQAGSGRNDETDAIAAFQRKGYQTVSNKLE
                     LERASGGRLLGLFSDKEMSFEIERKKNSEPSVYDMTRAAIRNLHDNNPRGFFAFIENE
                     NIDTASHLSDIAAMIQDYRELDRAVGLAYEFYKKYPRETLILVTSDHETGGLGFTMAL
                     KDLTSTKSENQVAATGAELRKLQAINISLQKASRILGPSPTAEAVDKLMRDYFSGFTL
                     APEFKEAIIAHQPPSRTLYIDPSAHALGLMIANNTQAYWQTSSHTNDPVAVAALGVGS
                     EQFKGYYDNADFGKKLLALLDGRTTPTLLRR"
     gene            complement(5504..6226)
                     /locus_tag="VF182_21225"
     CDS             complement(5504..6226)
                     /locus_tag="VF182_21225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439653.1"
                     /translation="MTARLIFAWLVHLYSALGAVIAFIAILLIEQLRFQEAFFLLTLA
                     VAIDASDGTLARAARVKELLPNFDGDRLEDIIDYFNYVVVPCLLLIRANLLPSQDAVW
                     LVAFPLVASAYGFCQKEAKTADHFFLGFPSYWNIVALYLYVLRAPLWFNAFLILGLSI
                     LVFVPIRYVYPSRSPYLRSWTNLLGILWGLAILAMIYWLPEPPRGIVFASFVFPIYYV
                     CLSLWLELRRVRLKRDPKSRLG"
     gene            complement(6235..6843)
                     /locus_tag="VF182_21230"
     CDS             complement(6235..6843)
                     /locus_tag="VF182_21230"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6439654.1"
                     /translation="MMIKLYTFPPSTNSRKVRIALIEKGLEFERVNVDLTKREQKNPD
                     YLKLHPFGQIPTLDDEGFVIYDSTIINEYLEDEYPYPPLMPKDSEGRARARLMEDFRD
                     NHFNAYFVHIIQEIRKPEGERDSQRTDNAKAEIAKAFDRIERELEGKEYLAGAFSLAD
                     IAFMPNIDLLDRFGIAVEPKYKSTIAWIERLKARPSFAASAS"
     gene            complement(7064..8917)
                     /locus_tag="VF182_21235"
     CDS             complement(7064..8917)
                     /locus_tag="VF182_21235"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6439655.1"
                     /translation="MRISNRNDLRRARLAGLFVTIGVLVAFSAWPVENPSLTSSVEFL
                     KAHARFLASEKLNGRSIDTPGIALAGDYIAAEFARLGLQPGGDNGSYLQPFEVATGVK
                     VTQPTSLTLGNQAPLRMDQDWTPLGFSASKKIEAAVVFAGYGITIKEYGYDDYRDIDA
                     KGKIVIVLRYEPPPKHANSPFRKYPNNSVHAALRSKASNARDHGAVGMILVDLHHQDN
                     AKPELISTRNSSWRGGTSLVAAHVSRASIEKWLETHGVSLTELRDKIDHEGKPASMEV
                     RAGKALLEVNLEEIRQRTKNVVAILAGSDPHLKSESVVIGAHYDHIGFGHYGTRNSSA
                     EGQVHPGADDNASGTAVLLELARRLTESDIKPLRTIVFAAFSAEELGLFGSRYYVNHP
                     AVPLSSTKAMINLDMVGRLRDDRVIVFGTRSAQELSDIVKSAAKQLGLQISDSDGIGQ
                     SDHMSFYSKKVPMLHFFTGVHSDYHAPGDTWDKLNLQGMATISELVLSTVWSIANTNT
                     PFKFVSLPSVPRPQAIGEGPTSTTYLGTIPDYSAEKEGVRLAGVSVGSPAALAGLREG
                     DVIVTLAETKVQNLEDLMLALGSKKPGDEVEIVVLRGTQELKLKAVLRARG"
     gene            9166..9351
                     /locus_tag="VF182_21240"
                     /pseudo
     CDS             9166..9351
                     /locus_tag="VF182_21240"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
     gene            complement(9566..9934)
                     /locus_tag="VF182_21245"
     CDS             complement(9566..9934)
                     /locus_tag="VF182_21245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX6439656.1"
                     /translation="MNDKELREEAARVLDELPEEFRKQLHNIEIVVQRRPSKHQLAEI
                     DLDSHEDVLYGLYEGVPLPDRSAFDPPILPDKITLFSEPLLSDFPDPDKLREQIRLTV
                     IHEIGHYFGMDDDEIEKLGY"
     gene            9981..11165
                     /locus_tag="VF182_21250"
     CDS             9981..11165
                     /locus_tag="VF182_21250"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEX6439657.1"
                     /translation="MRIPFLALCGLFSLLSLGIQIVHGQTVASTPPITDDLDRESLRL
                     AIHHSVSYLRKLPADQIVGEQPRPSTVGQVLDSLAALDRLLDRWSCWACFVKEFEAQF
                     ELVPSSRDEQHREVLFTGYYQPVIDGSLVETNEYRYPIYRKPTDLLTLERVAGDSGEA
                     GRKINGRVEREQFAPYYTRREIDHAGLLRGRGFEIAWVKDPIELFFLHIQGSGILRLP
                     DGTRLNIGYAGQNGRPYRSIGRLLIDQGQISQSEMSMARLRRHLEEHPEQRDEIMAHN
                     ESYVFFRVSEPGPLGSLDVPVTSGRSIATDSRLFSKGAPALIYSESPVLDSAGRLMGW
                     RPFVRFVLNQDTGGAIRGFQRADLYMGSGDDAGAHAGYMNSPGKIYFLMLKNPNRPGL
                     VR"
     gene            11259..12131
                     /locus_tag="VF182_21255"
     CDS             11259..12131
                     /locus_tag="VF182_21255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX6439658.1"
                     /translation="MERHSETRRLKWALAISLGYFFAELIAGFLTNSLALLSDAGHML
                     SDIGALVVSLFAFRMARRPATPESTYGYHRAEILAALFNGLALWLIVGVIFAAAYNRI
                     SNPPEVESFGMIVVAAVGLMVNIAAGFILYESHHHNLNLHGAFMHVISDAVGSVGAIV
                     AGLMMYFTGWYLADPLISVFIGLLILYSSWNLIREALSILMQSVPKTIRLEEVQRTIE
                     NVNGVMAVHDLHVWAVTSGIFTLSAHAVIGNGQDFHEVLNGIEETLKERFSIEHTTIQ
                     LETESREETEFKGF"
     gene            complement(12182..13402)
                     /locus_tag="VF182_21260"
     CDS             complement(12182..13402)
                     /locus_tag="VF182_21260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6439659.1"
                     /translation="MSAIAVFFGLGVLAPEKRRSLSLVYAASLALMMGVNFIQPALPA
                     MTEPFGISDSALSLIMTVFTAPAIVLSPIFGVIADMYGRRLLLAGGLILFGISGTAMA
                     FATSFTWLLVFRAIQGLGFSAVIPLTIVLIGDLLDGDEEIAGQGLKVFLDRVGYMILP
                     PLGGLLAALAWYWPFTLYIITVPLGIMTFFWMPETKGSVTSGTRAYLGEMLKLTRHPR
                     LLVAFSAGFLRFFLDYGFLTYFPLFLVRTHGISTATAGLLYVFFSAGAMITSSQAGRL
                     AAGRDKARVLFIAFLISGIALVAVPFMPGVWLVGGALFFYGIANGVISPMQKSLLTQN
                     APLEMRGGIVSFDRLIQQASKTISTSVVGLLLVTAELPTIFWMLGLLSLISVGLMALL
                     LPARRSAVMSPSAS"
     gene            complement(13493..14578)
                     /locus_tag="VF182_21265"
     CDS             complement(13493..14578)
                     /locus_tag="VF182_21265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HEX6439660.1"
                     /translation="MRQTGIAQLPLHGGKAPAWLFQRMVRLGREITRLIIENEGPEAF
                     LQHIADPFWLQSLGCVLGFDWHSSGVTTTVCGALKEGLKGREKELGLVIAGGKGATSR
                     KTPAEIVTAAEKYSLNCSPDALVYLSRLTAKVDNNALQDGFQLYHHSFFFTFNGAWAV
                     VQQGMNDRFARRYHWFSESVRSMVLEPHSAICSNLQKQRALNLVAMESQKAQSMITDL
                     SHERPEKLIGEIHRLQTLSLPSHHQVCVRDIHPNSIEKILLKTYERKATDFEELLSMP
                     GVGAKTLRALTLIADLAYGTPASWKDPVKFSFAHGGKDGHPYPVNCKLYDDSVEFLRR
                     TLERVRIDRSEKENALKRLQKLFASQN"
     gene            complement(14582..14827)
                     /locus_tag="VF182_21270"
     CDS             complement(14582..14827)
                     /locus_tag="VF182_21270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439661.1"
                     /translation="MTQPKKSANPKVTIIASKGANQAINYLLEDRDELEWLVAIGRNK
                     KGDIFFYDTGGDIIQDLGAIAYIKERILRDYFGETDE"
     gene            complement(14950..18549)
                     /locus_tag="VF182_21275"
     CDS             complement(14950..18549)
                     /locus_tag="VF182_21275"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEX6439662.1"
                     /translation="MPVNARQIFSRARDFTVHPRARKIAIWTVSLVVAFGVLLGVVAP
                     PLLRGKIARDLSQKLHREVSIEQIRLNPYAMTATVRGFLMKERESQATAVSFDELHVN
                     LELQSIFRLSPVIKELRLVKPYVNLVRNEDLKYNFQDIIDELTSGPSGGPTPKFALNN
                     IQLIDGRIEFDDRPEQTKHVISSINIGVPFISSLPSYTDIFVKPSFSADINGAPLHIV
                     GASKPFHSSHESTVDLNIDKLEINKYLEYSPVALNFKVPSGELNGKLNASFKSAKDSP
                     SVFTITGNLALTDLAVEKNGGTPFGKMANLDIAIASIDVFNNKTRLKSIKAHGLELHL
                     ARNRDGSLNTADLVTISPKTSSVEPKKDSKPLVYEVDDILIDSSTLHFMDETLQRPYK
                     TRLDNVRIALKGLTNEPAKQADLEVSFESNAKERISQNGKIQLEPFLAEGKLEIEGFN
                     PGNVSPYYQNAVALEVKEALLDLTTQYRFDTKDAGSDLKLTDLNATLKTVRLDLPGQP
                     EPLWRIPSLIIKDGSVDLLRKTVVIGAIQGKDGIGHVQRDKDGTLNYARIVKAQSSAP
                     VKEPVKKDETEWKIEAKQIALDRFKVDVDDRGAATPAKFTLSDLFVRIDNFSNAKNQP
                     GKTRLRTRINKNGALRVNGTAATNPVSAKLAVEAYDIDALSLQPYFGDQVNFLLTGGR
                     ISTKGSFTLVDGGTGPAKVNYQGGIELYDFASIEKDNKQDLVKWKNLGLHQFELTSEP
                     LQVRIGEVDVTDFYSRVIIGPDGKINLQNLKASKDDNQENAPPTQTVAAPGTDAPDTA
                     KPFTIGKINLHGGNINFSDFLIKPNYSANLTDVHGTISELKPEAPGDIDLQAKLDGAA
                     PVDIDGKINPLGEELYLDIVADAREIEMNPFSPYSGKYVGYGIESGKLSFNVKYKVEN
                     RKLTAQNKIILNQLTFGEKVESPQATKLPVLLAVALLKDRNGVIDVDLPISGSLDDPQ
                     FSVGGLVLRLVINIITRAVTAPFALLGSAFGGGSGGGEELSYVEFDSGRATLSQTAQK
                     KIETLAKAMNDRPALRLELSGRADPAADLDGLKRVSMERKVKAQKLKELSGKSEAPRS
                     VDEVRIESSEYPQYLRQAYRQESFPKPRNAIGLLRDLPVVEMEKLMIQHATVGEEDLR
                     ELAGQRAQTVRDALVATGQVSADRLSIVAAKPPTNEEKAMPKGKPNRVDFSLR"
     gene            complement(18721..19161)
                     /locus_tag="VF182_21280"
     CDS             complement(18721..19161)
                     /locus_tag="VF182_21280"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX6439663.1"
                     /translation="MYYFAYGSNLNWPQMQRRCPSSRFVCIARLPDYQFGITRHSRLR
                     SCGTANVIAARGKEVWGAVYDICDEDLLILDSFEDGYRREVLPVFGLNGEKAPLHALV
                     YIAELELDVPLPNAEYKRLILEGARHWKLPSLYLSVLEGIKAEK"
     gene            complement(19266..20564)
                     /locus_tag="VF182_21285"
     CDS             complement(19266..20564)
                     /locus_tag="VF182_21285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6439664.1"
                     /translation="MTTALKSKRPRFFYGWHIVAVAILVNIAGTFAFSSTLSIFLKPI
                     TAELGVSRGAFSLIRTFEIGIAAIIVPLLGPWVDRHGGRGIIVIGVLMEGAGLLLSSI
                     VQTFWQFVLVRCSLVIAGEALLGSLVINVTIAQWFVRKRGRAMGIANLGTGIAKLAIP
                     LIAAWLFVLIGWRGTWAVFGTVAPLLVVAPALIFIRRRPEDMGLRPDGEPPRERFEAV
                     PPTGQRSSNAQPRRAYGEIVWNRSDVLRLPVFWLLVVTFGIVSVGIAGLNLHIFSYVT
                     DIGYSPLIAASFMSTIALTQLGSTLVWGVLADKFDLRKVSSIQFLIQGAGLVTVIASI
                     DIRFVYLGFFLYGTGLAGSFVLREVIWANFFGRVSLGKVRGLSLFFSHLFAASGAPFF
                     GFLHDRMGSYNISFTIFSCALFTSAFLILFAKPPIKREED"
     gene            20812..21891
                     /locus_tag="VF182_21290"
     CDS             20812..21891
                     /locus_tag="VF182_21290"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439665.1"
                     /translation="MLILSNEDIEKILPVGACLEVLEEAYRDLGTGMAATVPRYDVFS
                     PTKPSEFYEYKTMSGVLPNRKIAALRLNSSVVTWYEKAGGLRKDKLPVAGGDRYVGLV
                     MLFSTETGEPLAIFPDGYVQKLRVAGASAIAARHLARKNASVMALLGSGWQASAHLIA
                     LSLVRDLKKVIVFSPNAESRNHFLDEWSDKVSTELVAAVSAAEAIDSADIITCATNSI
                     SALFPGELLKPGVHVSCVKPCELDATTYRRSDPLIIHWKEAKPFQIVIGGVDPQSIPD
                     VADGWPHPLTREDTAVWDLPTISQLVNGQHPGRTKDEAITCFCNNVGLGLQFAAVGSE
                     VLARAREARVGREIPTGWFLESVHP"
     gene            22033..23025
                     /locus_tag="VF182_21295"
     CDS             22033..23025
                     /locus_tag="VF182_21295"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439666.1"
                     /translation="MKIKIALLTLLLVFFATAWAAMAQTLKLNVAYPTTVGSMAVLWV
                     TKEARLFEKHGLQVELIYIGGSSKVVQAILAREIPIAEIAIPAVVQANLAGADLVMLA
                     GPNHKPGQKIMVTPEIKKPEELKGKKIGVTRFGTSDDFLLRYVLGQWGIAPDREVALI
                     QMGGSQETLAGMIAKSVDGGLLSSPLHLRAGKLGFSMLADLSAIGVDYQGAGVVTTHR
                     YLRENPDTIHRYLRAYVEGLHRLKTDKAFSMKVIAKYSRISEAAALEETYQHYAVKVM
                     PRVPYPTTKGIQMVLDEMSARNPKAKSLQPGSFIDVANLKELEQSGFIKRLYGE"
     gene            23234..24610
                     /gene="tilS"
                     /locus_tag="VF182_21300"
     CDS             23234..24610
                     /gene="tilS"
                     /locus_tag="VF182_21300"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX6439667.1"
                     /translation="MLSPGQRILATVSGGPDSVAMLHLLCDLREELELTIEVAHLQHG
                     IRGEEALEDARFVARLAEQLGSICHLKEVDLPRLKVASGKTSLEEIGRFERYRFFAET
                     AHRRGLDAVATAHTADDQAETFVMRLFRGAGRTGLRGIMPVGQLAIGLERPCANVLLI
                     RPLLDTRRREVMEYLDQRNLTYRTDSSNKDLFFLRNWIRLKLMPQVSERFGHKLTERL
                     CAQAQVLREEEDYLAEVTRQQFENVRDGQRLNRKLFLNLNRALQRRVIRLWIAQRRGH
                     LRAIDFDHIEAAQSLIADGPPQGRLALPGGWQLARQYETITFERGRPNIKPQCYSYLF
                     KPGISLPVVEAGIRIESSLIDPAPGQLPDNSMEALFDADLLKGELIVRNFRNGDRFQP
                     LGMGGHKKVKDLFIANQLPLHTRASLPLLVMDNEILWIPGYGRSDFARIGSTTKISLH
                     LKAIPQPK"
     gene            24682..26520
                     /gene="ftsH"
                     /locus_tag="VF182_21305"
     CDS             24682..26520
                     /gene="ftsH"
                     /locus_tag="VF182_21305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015337054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX6439668.1"
                     /translation="MSQSSKHIALWLVLLLVLLGIFSVFSKQAGRDPEVVFSEFLLAV
                     ERGEVQEVVIQGHNIQGKYKNGERFRTFAPDDPELVKSLRDKKVKIAAKPEDDSPWYY
                     VLLINWFPMLLLIGVWVFFMRQMQVGGGKAMSFGKSRAKLLTENQHRVTFSDVAGAEE
                     AKDDLQEIIAFLKDPKKFTRLGGRIPKGCLLVGPPGTGKTLLARAIAGEAGVPFFSIS
                     GSDFVEMFVGVGASRVRDLFVQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTL
                     NQLLVEMDGFEANEGVILIAATNRPDVLDPALLRPGRFDRRVVVPRPDVKGREGILQV
                     HTRKVPVASDVDIAVLARATPGFAGADLENLVNEAALLAARNNKDKVDMRDFELAKDK
                     VMMGAERRSMIISDEEKRITAFHEAGHALVAKLLPGADPIHKVTIIPRGMALGLTQQL
                     PMDEKHTYPKEYLLNNLVILFGGRVAEELVLNHMTTGAGNDIEKATELAHRMVCEWGM
                     SEKLGPMTFGKKEEEIFLGRDFTQTADYSESTAIEIDSEVRRIIQESYHKAKDLLKTN
                     LALLHKVAESLLEREVLDGSEIDAIVRDFSGNGGDPLNPTRAAAVA"
     gene            26885..27793
                     /gene="folP"
                     /locus_tag="VF182_21310"
     CDS             26885..27793
                     /gene="folP"
                     /locus_tag="VF182_21310"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX6439669.1"
                     /translation="MNSSIDPIDIADHQPGRALATRHGLLDMTCHTVVMGIVNVTPDS
                     FYDGGKRFDGARAIAGAIEMIDSGAEILDIGGESTRPGAQPVSVDEELRRVLPVIREL
                     RQRSSVPISIDTYKEAVARAALDAGADIVNDISALRFDPAMAALVARSGVPVILMHMQ
                     GVPQTMQREPRYQDVVLEVQDFLAARVRSASLAGIAREQIIIDPGIGFGKTLVHNLAL
                     LKDLHSLKSLGQPLLIGVSRKAFIGRILNSAAPEERLEGSLAAAVAATLSGANIVRVH
                     DVSETVRALRVTDAIRLGRLPDLGER"
     gene            27790..28545
                     /gene="cdaA"
                     /locus_tag="VF182_21315"
     CDS             27790..28545
                     /gene="cdaA"
                     /locus_tag="VF182_21315"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEX6439670.1"
                     /translation="MTEILGQLRWQDALDISIIWYLIYRLLQMLRGSRAMQMMIGLAV
                     VLMAYVLSRGLGLFTLNWILDNFLASIILVIIVIFQSDIRRALTQVGTAPIFGAEKMV
                     QRREDIIEEVAEAVRSLASRRVGALIVLEREVGLNEYIEIGTRLDARVSRELIESVFL
                     PGSPIHDGALVVQKRLVTAVRCLLPLSANPNLRTTMGTRHRAAIGVTEETDAVAIVVS
                     EQEGTIALVVGGNLTERIEASRLRNALEGLVKL"
     gene            28542..29471
                     /locus_tag="VF182_21320"
     CDS             28542..29471
                     /locus_tag="VF182_21320"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEX6439671.1"
                     /translation="MKEIWRQIGSTASSNVGLAVLSFIIALGLWIAGHRDIERAIEVP
                     VEFRNIPSDLMVLDNRVDYVVLRLSGPRTLVSTLDADDLKLQVDLYGSKSGSLSFPLG
                     PSSFNIPRGVTVARITPPIIHLRLDPVIKRQLPVSIRLSGKPLTGYKIEEMAVQPESI
                     SVRGPADEIRRMGLVETLPVDIEGSRNSIKRTVRLSTDGKPLSFMPEQVSVAITIVEE
                     DSIKDFKNVEIGARGASGDYVLTPKSAQVRLFGPRRILDRLELGADQVYVDLDGLATG
                     EHAVDLKFNLPAEIKVVEQKPARFKVRINKSHP"
     gene            29492..30943
                     /gene="glmM"
                     /locus_tag="VF182_21325"
     CDS             29492..30943
                     /gene="glmM"
                     /locus_tag="VF182_21325"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX6439672.1"
                     /translation="MAQVSKQKRRLFGTDGVRGIANQEPMTSEMALKLGRAIAKVLQD
                     PIARPAQSSGRSKSPSLAKTLGGDTAHRYKILIGKDTRLSGYMLETALASGICSMGAD
                     VQLVGPLPTPAVAFLTRTMRADAGVVISASHNPYQDNGIKFFSFDGFKLPDEVEARME
                     EMIFSGEADRERPTASQIGKAFRISDAVGRYNVFVKNSFPRHLTLEGLRIVVDCGHGA
                     AYRVVPEILSELGAQVIPIGVQPDGENINHHCGALYPEAARDVLLHEKADFAVSLDGD
                     ADRAIFIDEDGEVLDGDQVLAICAKDMQEKGTLKDRTVVATVMSNLGLDLALKSIGVN
                     LERTEVGDRYVVERMLERGYNLGGEQSGHILFLDHNTTGDGAITCLQVLALMVDTGKR
                     LGELKRIFTRLPQILLNVRVTERKEFQAMPKVRRAIASVEQSLNGRGRTLVRYSGTEM
                     LARIMLEGEDEDRICAMAQEIADEIRTEIGVKD"
     gene            30940..31662
                     /locus_tag="VF182_21330"
     CDS             30940..31662
                     /locus_tag="VF182_21330"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX6439673.1"
                     /translation="MIRLGVNVDHVATVRQARRVDVPDPLEAALIAEKAGADGITVHL
                     REDRRHIQERDVQLLRERLNTKLNLEMAVTPAMLIFAENVRPNDACFVPEKREELTTE
                     GGLDVVSHRQKIREATQRLQGIGVRVSLFIDPESAQIEASRDAGAHAVEIHTGAYCNA
                     LGMEREKQLQVIREAGKLAQSLGLEVHGGHGLNYDNVLAIAQIPEMAELNIGHSIIAR
                     AIIVGIEQATREMKDLLSKAKE"
     gene            31760..33313
                     /locus_tag="VF182_21335"
     CDS             31760..33313
                     /locus_tag="VF182_21335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX6439674.1"
                     /translation="MILVTAEQMREIDRLTIQKYGTPSLTLMERAGAGVTHAILPRFA
                     KPLSKGVLIVAGKGNNGGDGFVVGRLLRQQGVFCEIALLAKRNETSSDAAKNLQAYLT
                     AGGKVVDIEADRLPLLNDAIAGKGLLVDAILGTGTKNEIRGLYAEAIRAMNESGLPIV
                     AIDIPSGLDADKGTQLGVCVQAAMTVALAYPKIGEVIHPAVEYVGELVVADIGVEPAA
                     VTEVAPAAELLTREEIRPLVPVRKPDSHKGTYGHVVVIAGSRGKTGAAILASRACMRA
                     GAGLTTLATPRSLNDVFAGAMIEVMTEPLRDTAEEEIESLTDEEWWRLLERKTVALFG
                     PGIGVTDSTRNNLRWLLRHLSIPWVIDADGLNNLALDIDRLRKARTAPILTPHPGEMA
                     RLLGKDAVTVNKDRIGVARAFAAEHRCYVVLKGARTVISTPGGKIFINPTGNPGMASG
                     GLGDVLAGILAALLAQGLSPEAAMKLAVYLHGFIADRIAAERGMLGMIASDIIDGLPA
                     GLRSLSAVV"
     gene            33367..>33797
                     /gene="tsaE"
                     /locus_tag="VF182_21340"
     CDS             33367..>33797
                     /gene="tsaE"
                     /locus_tag="VF182_21340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX6439675.1"
                     /translation="MQWAVISRSAEHTRRLGVRLGKLLQGGEIVGLIGELGTGKTCFV
                     RGLTEGLGVSQETWIRSPTFTLVNEYHGRLPIYHIDLYRIDSTGELEELNLREYLYSN
                     GVSLIEWFEYFPAGEVDDHLAIQLAYREGSKRQLTFVPPGAR"
BASE COUNT         7677 a         8703 c         9530 g         7887 t
ORIGIN      
        1 acgcggtaac aataggttgt actacttgcc aggttcgcat ccgtatatgt actaatgtta
       61 gatccaacac tagccacgcg tgcgaagatg ccgctagtcc cggttttccg ttcaatagcg
      121 aaaccatcct cattatccga agcgtcagtc caactaagtg ttagctgagc ggcagaggca
      181 atgtgatgtg tgaataaatt cgcaactagc gcaagtgcga gaataagcca gaagcttggg
      241 cggaaataca gcattacttt cctccgaaga atggattcaa tggatgcact gatgccgcat
      301 ccttgcgtat gatcgatgtt tctttatcgg ctatcacttg gtacgaacga ctggctgcgg
      361 cctgttcgaa ggccgccgga gcagcatcac tcagttttgt aaggctgctt tgtgtttgtg
      421 gctctgttgc ggatctcgac gttgcgagaa ctgtttcagc aacacatatg ccattagact
      481 cgtggttctt tgcttccccc gcatgagtca gtttaggggt tcgaaggaag ttttcgtctg
      541 atattgcgca cgaagtattc gcgctgttgg cgacattact gttcgcttag gtccgggggc
      601 gggctcgtgg gacgaagaac cgaatattct tctacgatta gtcgaacgat gtgtaacgcc
      661 ggaatgctct tccagagtgg gggttgaggt agtgtgctta caactggatt aatgcattaa
      721 ttacattgat gctcgcttaa gttacaagta agcggtagat aaccacctgt tatgtttcct
      781 ttgagcgctt aggaccgtta aacaggaaaa atagtgtcaa cgaagtccat cggcttaaga
      841 caaagcctac aagacggttt aactcgagaa atacttgcgt gagtttgatg atgctcagct
      901 gatacgactg aatgtacgtg gcagcctgag tttgtttcgc gggctcggca gctgtgcagg
      961 tttgttgatg agagatggtg ataagcaagc gagttcttga agttgctgat cctgctaaag
     1021 gatccaactc ttctggtgga ttctttgtaa ggctattttt tcgaagtgtc ttgcatcttt
     1081 tctccggtct tctgcatgga ctcgcccact cgttccatta gcttaccggt ttcctgacct
     1141 gctttctcga gggcgttgtc gatcttttta ccggcttttt ctgcagagcc ttcgccatcc
     1201 tcgctccttt tacaaccggc aggcgcaatc atagccgccg caatggctaa gatcagcagt
     1261 cttctcttta tcttcttcat agggaattac agcctccgtt aacatgtatt atcaagtcgc
     1321 tttatttgcc aactcgcgat gtgacgcata caggcgggta cagtcggtga ataaacagga
     1381 tgtgactgcc gggataacct tgactgagtc aatccagttg gcgtttgatt cagtttcgcc
     1441 ctcggttgat aaaactgatg cggcgcccag cgtgcgtaac tccgcttaaa gcgatgcccg
     1501 gttaactttc ccatcggact tgaaagccat ctttgctcaa ttcgcttcta atccgttgga
     1561 catgatccaa gccgccggtc tcgatcacta gttcgacttc cgtttggtcg atggcaattc
     1621 ggccgaagcc gcggctctgg ccgatttgca agatgttggc gtcgaggtcg gcgagtcgct
     1681 ggctaagcct cgccaggttg ccgggtcgat cccgaatcac cacggaaaat cggctcagtc
     1741 gcccatcttg aatgagtcct ttttcgataa tccgggatat caaattgaca tcgatattgc
     1801 cgcctgaaac gatcagggca acgttcttgc cgatcaagtt aactttcttg ttgatgagcg
     1861 cggcaagagg agccgcagcg gcgccttcgg ccacagtctt ttctatttcc agtaaaagca
     1921 gcactgcatt ggcgatttca ccttcggtaa ccgtgacgat ctcgtcaacg tgcttttcaa
     1981 caaggtccaa cgctgtctcg cccactttac gcacggcaat cccgtcggcg attgtggttg
     2041 ccggctcgag tgaaatcgga gcgccttttt ccagggccgc tttcatcgat ggtatcgcct
     2101 ccgcctgtac gccgatgatt cggatgtgcg gattaagaac cttcaggaca agtgcgatac
     2161 cggcaatcaa gcctccgccg ccaacgggca caatgatcgc gtccagatgt ggattctgct
     2221 catacagctc gagggcgatc gtgccttgcc cggctatgat cgttggatca ttgaacgcat
     2281 ggagtaacgt caaccctctt gcttggctca gtcgctgggc ttcggtgaaa gccccgtcgt
     2341 aatcttcgcc atacaagagg gtgtgtgcgc catatagccg gacccgtccc actttgatca
     2401 atggcgcatg aagcggcatg actatcgttg actctacatt cagccggcgg ctgtgaaatg
     2461 ccactgccat accgtgattg ccggcgctgg cggcaatcac gccgcgctcg gcttcgtctt
     2521 gtgtcaaact cagcaaacgg ttgagcgcgc cccgttcttt gaaggaaccg gtcatttgga
     2581 gattatccaa tttgaaataa accttattgc tggtcatccg gctaatggtt tcagaatagg
     2641 agagaggcga gaggtacact tgatcgcgga tgcgccgtcg cgcttcctca acatcgacga
     2701 aactcagcat gatgcataac ctagaatgaa acagcccgcg cagcaaggcg agtgaataaa
     2761 gttgactagg caagccgccc tatggcaagg atgaaccatc tgcggcagcc tagcgttcta
     2821 gtaaatgaac gaagccccta tagaaatgaa gttaaataat cgtcttggag gcgacgtgtg
     2881 atgcctacgc aggagaaccg tgcggcggat gttgcgcgtt accgacagaa ttacctcgtc
     2941 gaaatggacg gcattgagct ctatcgtgca ttggccgccg ctgagaagga cgaaaggcgc
     3001 gccgtgattt tcagaaagat ggcgcaaact gaagagcggc atgcaaggcg ttgggtgcgg
     3061 ctcatcgaga cggcgggttt cacagtgcct gactaccgtc cgactgttcg tgtccgggtg
     3121 ctagcctgga tggcaaagca ctttggcacg catcgagtga ttccgatcat cagtagcctg
     3181 gaagccagcg atgagccggg atatttgaat cagccggagg ccgtcggatt gccggcccag
     3241 gagagagccc atagccgcgc gttgcgggcc atggacgacg ggatctccac ccaggatgca
     3301 attatcggca gcgaacgatg gcatgtgatg tcccgcgggg gcagcctgcg tgccgccgtg
     3361 tttggcatca acgatggact agtttcgaat ttcagcctag tcatgggctt tgcgggcgcg
     3421 gatgccagac ctgagtatat tgtgctagcc ggcgtagccg gcttattggc gggatcgttt
     3481 tcgatggcag cgggtgaata cgtttcgatg cgcgcgcagc gtgagctctt cgagcagcag
     3541 atagcgatgg agcaacaaga gctggagatg tcgccgaagg aggaagagga ggaactgtcg
     3601 ttgatttatc aagccaaggg aattcctgaa agagaggctg cgatgctggc gcggcgaatt
     3661 atcgccaacc cgaaaaccgc tatcgacacg ctcgcccgtg aagagctggg gttgaatcct
     3721 gcggaactcg gatcgccttg gagcgcggcc ctgagctctt ttctggcttt catacttggc
     3781 gcgatgattc cagtgctgcc ttatctgttt agtaccggac agcttgcctg gtttgtgagt
     3841 ggtttgctca gctgtctagc actattagga gtgggagcga ttatttcgat ctttaccgcg
     3901 aaaggtccgt ggataagcgg gctacggatg ctgggcatcg gcctcctggc atcggcaatc
     3961 acttacggcg tgggatggtt gtttggagta gccgtgccgg ggtaggttga cgcaccgcgg
     4021 gcggcaccgc gcctgctctc atcgcgcgtc gtacgcattg cctatcgtcg gagtagagtc
     4081 ggagtcgttc tcccgtccag gagcgctaac agctttttgc cgaaatcggc attgtcatag
     4141 taacctttga attgttctga gcccactcca agtgccgcga ccgcaactgg atcattggta
     4201 tgagaggaag tctgccaata cgcttgggtg ttattggcga tcatcaatcc cagagcgtgg
     4261 gcgctaggat cgatatacag cgtgcgcgat ggcggctggt gcgcgataat tgcttccttg
     4321 aattccggtg ctagcgtaaa tccgctaaag tagtcccgca tgagtttatc gacggcctct
     4381 gcggtgggcg acggcccgag aattcgcgat gctttctgaa gcgaaatatt gatggcttga
     4441 agctttctca gctcagcgcc ggtggctgcc acctggttct ccgattttgt gctggtaagg
     4501 tctttcaagg ccatggtaaa gcccaagccg ccggtttcat gatccgaggt gaccaagatc
     4561 aacgtctccc gcggatactt cttgtaaaac tcataggcga gtccgacggc gcggtccaat
     4621 tcgcggtaat cctggatcat cgctgcgatg tcggagagat ggctggccgt atcgatgttc
     4681 tcgttttcga tgaatgcgaa gaatccacgc ggattattat cgtgcagatt acgaattgcc
     4741 gcacgagtca tgtcgtagac cgacggctca ctgttttttt tgcgttcgat ctcaaagctc
     4801 atttctttat cgctgaacaa cccgagaagt ctgccgcctg atgcacgctc gagctcgagt
     4861 ttgttgctga ctgtctggta accttttctt tgaaacgcgg cgatcgcatc ggtttcgtcg
     4921 ttgcgcccgc ttccggcctg gctcttcgga atgaagaact ctttgccgcc gcctaaaatc
     4981 acgtccggct cgagctcaat atagcgatcg atgatcgagg cgaaatgccg gcggttagga
     5041 acatgacaga caaatgcagc agggctggcg tcgtagacgg ttgaattagt cacaagtccg
     5101 atgcgcatgc cgcgtcttct ggcatactcc atcaccgtat caggcatgga accgtcggcg
     5161 catacaccca atgcgccaat cttggcttta cagccgctgg ccagcgccgt cgccgcggcg
     5221 gcggagtctg tggagagaga attcgccgca tgggtagtca tcaatccgac gcttccttct
     5281 gtcatgatct tgtcgctgat caccattcct tcgttatgaa cttgccggtt gtattgacgc
     5341 gtaatctcga ggtgagcgat gccgccaccg tctgcaagga atagaaaaac gtattttggt
     5401 gaagccggct gggtttgggc cgcgcgcgcg cttatcacga cccagccacc cactacgagc
     5461 aaggccgcga ttacgcacgt gagggagaag cgagattttc tagtcatcct aatctggact
     5521 ttggatctct tttgagtctg acccgacgca gttccagcca caaggaaagg cagacatagt
     5581 aaataggaaa aacaaaagaa gcgaagacaa tcccgcgtgg cggttcaggg agccaataaa
     5641 tcatagccag gatcgcaagg ccccacagaa tgcccagcag gtttgtccat gaacgcaagt
     5701 acgggcttcg gctgggatag acatatctaa tcggcacgaa cacaaggatc gatagcccca
     5761 gaatcaaaaa ggcgttgaac caaagcggcg cccttagaac gtagagatat aaggcgacaa
     5821 tgttccagta cgacggaaag cccagaaaaa agtgatccgc cgttttcgct tccttctggc
     5881 aaaaaccata agcgctcgca accaggggaa aagcaaccaa ccagaccgca tcctgagagg
     5941 gcaacaaatt ggcgcggatg agcaagagac acggcaccac gacatagttg aagtaatcaa
     6001 taatgtcttc cagtcggtct ccatcgaagt tcggcaacag ctctttgacc cgcgcggctc
     6061 tggccagagt gccgtccgaa gcgtcgattg cgaccgccaa tgtcagtaga aaaaaagctt
     6121 cttgaaacct tagctgttcg atcaagagaa tcgctataaa cgcgatcact gccccgaggg
     6181 cgctgtatag atggactaac caagcgaaga tcaggcgtgc ggtcatccag ccgcttagct
     6241 tgctgaagcg gcgaagctcg gtcgcgcttt tagacgttcg atccaggcaa tcgtgctttt
     6301 atacttcggt tcgacggcga tcccgaaacg atccagcagg tcgatgtttg gcataaaagc
     6361 gatatcagct agactgaaag cgccggccaa atactctttc ccttctagtt cccgctcgat
     6421 tcggtcgaaa gcttttgcaa tttccgcctt cgcgttgtcc gtccgttggc tgtccctttc
     6481 gccttccggt ttgcggattt cctgaatgat gtgaacgaag taagcattaa agtggttgtc
     6541 gcgaaaatct tccatcaagc gcgcgcgggc gcgcccctcg gagtctttgg gcatgagcgg
     6601 cggataggga tactcgtcct ctaggtattc gttgatgatg gtcgaatcgt agatgacaaa
     6661 cccctcgtcg tccaaagttg gaatctgacc gaacggatga agttttagat agtcggggtt
     6721 cttttgctcc cgcttggtga gatccacatt gacccgctca aattctagtc ctttctcaat
     6781 taatgcaatt ctaactttgc gggaattagt cgacggtggg aaagtataca acttgatcat
     6841 catcagcctc ctagaagtgt caccacagca ttcactgaaa ttatactaac ataaggtatt
     6901 acacgctgcg agcatttttc ctacgattgg aggctaaaac atttgtatct gccggcgaat
     6961 tcacgatgat aatcgctccc gagaggacga cgtgatcgta gcaccgtgct gatctagccg
     7021 ttcgcgatgg aaacagttta acgcccgcga gttacggcac cggctaaccc cgagcccgaa
     7081 gcactgcctt aagcttcagc tcttgtgtcc ctctaagcac gacgatctcg acctcatcac
     7141 ccggtttctt gctgcctaag gcgagcatca gatcctccaa attctgaacc ttggtttcag
     7201 ccaacgtgac gatgacgtct ccctcacgga ggccggcgag cgcagccgga cttccgaccg
     7261 aaactccggc gagccgtacg ccttccttct ctgcggaata gtcaggaatg gtgccgagat
     7321 aggtcgtcga agtcggtcct tcgccgatgg cctgcggcct tggcactgat ggcaggctga
     7381 caaacttaaa tggcgtattg gtgttggcga ttgaccaaac tgtagacaga accagttcgc
     7441 tgatcgtcgc catcccttga agattaagct tgtcccaagt atcgccgggc gcatgataat
     7501 cggagtgcac gccggtaaag aaatgaagca tcggcacttt cttgctgtaa aacgacatgt
     7561 gatcgctctg gccgatccca tccgagtcgc ttatctgcaa ccccaactgt ttagccgcac
     7621 tcttgacgat atcgctgagt tcttgggcgg aacgggtgcc gaatacgatc actcgatcgt
     7681 cccgtaaacg gcccaccatg tccaggttga tcatcgcctt ggtcgaagat agggggacgg
     7741 caggatgatt gacatagtat cgcgatccaa acagtcctaa ctcctcggcc gagaaggcgg
     7801 caaaaactat cgtccttaaa ggtttgatat cgctttcggt cagtcggcga gcaagttcca
     7861 gtagtacggc ggtgcctgag gcattgtcat cggcgccagg atgaacctgt ccctcggcgc
     7921 ttgaattacg cgtgccatag tggccaaagc caatgtgatc gtaatgtgcg ccaatcacca
     7981 cgctctcact tttgagatga ggatcggaac cggcaagaat ggcgacaacg ttcttggtgc
     8041 gttggcgaat ctcttccaga ttcacctcta acaaagcctt gcccgctcgc acctccatcg
     8101 atgcaggttt tccttcatga tcaattttgt cgcgtaactc tgttagcgaa acaccgtggg
     8161 tttccagcca tttctcgatt gacgcgcgac ttacgtgcgc cgctaccaaa ctggtgccgc
     8221 cccgccacga gctatttctg gtggagatga gttctggttt ggcattgtct tgatgatgca
     8281 gatccaccag gatcatgccg acagcgccgt gatcccgcgc attacttgct ttgctacgga
     8341 gcgcagcatg aaccgaattg ttgggatact tacggaacgg actgttggcg tgcttgggcg
     8401 gcggttcgta gcgcaggacg atgacaattt tccctttggc atcaatgtcc cgatagtcat
     8461 catagccata ctcttttatg gtaatgccat aaccggcgaa aacgaccgcg gcttctatct
     8521 ttttcgaagc cgagaagcct aatggagtcc aatcctggtc cattctcaga ggtgcttgat
     8581 tgcctagcgt cagacttgtc ggttgcgtga ccttcacacc ggtggcgacc tcaaacggtt
     8641 gcagatagct gccgttgtca cctccaggtt gcagaccgag cctggcaaac tcggcggcaa
     8701 tatagtcacc ggcaagcgct atacccggag tatcgatgct ccgcccattg agcttctcgg
     8761 atgcgagaaa acgcgcatga gcttttagaa actcaacgct ggaggttaaa gacgggttct
     8821 cgacaggcca agcagaaaac gcaaccagta cgccgatagt aacaaacagc ccagctagac
     8881 gagcgcgtct caagtcatta cgatttgaaa ttcgcatatc attgcctgtt cagctatgac
     8941 aaagttgtca ctaaactaac aaaccggccc cggctttgaa atatgcatct cgagctgcga
     9001 ttggaaagaa ggttcatcac tggatgaccg ctcctcggtc gccgccggca gcttccatca
     9061 cgggttgcaa aaagatacta tacgttgaaa ctactgaggg aggtaaatgt ggcggcaaaa
     9121 acaatcgttg tctacaccca gcccggttga ggaccttgtc acaaagaggt ggagtttcta
     9181 tctcaaaagg gcgtcgagtt cgtggaaaaa aatgtgcgcg ccgataaggc ggcattgaaa
     9241 gagttgatcg acatgggcta tcaaagcacg cccgttacaa ttgtcgatgg agaagcggta
     9301 gttgggtttg atcagctaaa aataatggag ctcatcggca tcgaaaccta agcggtcgaa
     9361 agttgccgac gccggcatta tcagatcaaa ctgcggcaac gcgcaaagcc gggctaaatt
     9421 tcccgccgag atcgaagcgg gttcactttg gattttttgg agtttgattc ggcggatgaa
     9481 tccgatgcat ggtctgcggc agagagcagg catttagttt aagactgttt ttttcgttcg
     9541 attctgattc gtccctagtt ctgatctaat accccagctt ttcgatttca tcgtcgtcca
     9601 taccgaaata gtggccgatc tcgtgaatga ccgtaagtcg aatctgttcg cgtagtttgt
     9661 cgggatcggg gaagtcgctg agcaggggtt cggagaatag tgtgattttg tccggcagga
     9721 ttggcggatc gaatgccgag cggtcgggca aaggaactcc ttcgtacagt ccatagagaa
     9781 catcttcgtg agaatctaag tcgatttccg ccaattggtg tttgctcggt cttctctgga
     9841 cgacgatctc gatattatgc aactgctttc ggaactcctc gggcagttca tcgagcacgc
     9901 gggcagcttc ttctcttaat tctttgtcat tcaatgctag aatgcttgca ctaattggca
     9961 aacgacttca aggagtatcc gtgcggatac cattcctagc gctgtgcggt ttattttctt
    10021 tgctgtcgct cggaatacaa atcgttcatg gccagacggt tgcgtccacg ccaccgatca
    10081 ccgatgacct cgatcgtgaa tccctccggc tcgcgatcca tcacagtgtg tcttatctgc
    10141 gcaagctgcc ggccgatcaa atcgtcggag agcaaccccg cccgagtacc gttggacaag
    10201 tcttggacag ccttgctgct ctcgatcgac tcctggaccg ctggagctgt tgggcgtgtt
    10261 tcgtcaaaga attcgaggcc caatttgagc tagtgccgtc gagccgagat gagcagcaca
    10321 gagaggtatt atttaccggg tactatcaac cggttatcga cggcagcctc gtcgaaacca
    10381 acgaataccg gtatccgatt taccgcaaac ctacggatct cttgactctg gagcgagttg
    10441 caggagattc cggcgaggct ggaagaaaaa tcaacgggcg agtcgagcgc gagcaattcg
    10501 ctccttacta tactcgccgt gagatcgacc atgcaggatt gttgcgcggg cgtggtttcg
    10561 agattgcctg ggtcaaggat cctatcgaat tattttttct tcatattcaa ggctcgggta
    10621 ttttgcgcct gccagatgga acgcggctca atatcggtta tgccggccaa aacggccggc
    10681 cgtacaggag catcggccgg ctgctcatcg atcaaggcca aatctcacaa agcgagatgt
    10741 cgatggcgcg attgcgtcgc catttggagg agcatcctga gcagcgtgat gagatcatgg
    10801 cgcataatga gagctacgtt tttttccgag tcagcgaacc aggtccattg ggcagtctcg
    10861 atgtgccggt tacatcggga cgttcgattg cgaccgattc gcgtcttttt tcaaagggag
    10921 ccccggccct gatctatagt gaaagtccgg ttttggatag cgcaggtcga ttgatggggt
    10981 ggcgaccttt tgttcgcttt gtcctcaatc aagataccgg tggcgcgatc cgcggttttc
    11041 aacgagcgga tctttatatg ggcagcggcg atgacgcggg cgctcatgcg ggctacatga
    11101 atagtcccgg aaaaatttat tttttgatgt taaagaatcc caatcggccc gggctagttc
    11161 gttagttaga gcgcggctcg gggctgccat ccgagtagcg gcgttttccc gttgaaactc
    11221 taccggccgg cagagtagcg tactaaaata catcaggtat ggaacgacac agtgagacgc
    11281 ggcgccttaa gtgggcgctc gcaatcagcc tgggctactt cttcgcggaa ctcatcgccg
    11341 gttttctcac caatagcctg gccttgcttt ctgatgccgg gcatatgttg tcggacatcg
    11401 gcgccctcgt cgtcagcctt tttgcctttc ggatggcgcg gcgtccggct acgccggaga
    11461 gcacctacgg ctatcatcgc gcggaaattc tggctgcgct gttcaatggc ctggcgctct
    11521 ggctgattgt cggagtaatt tttgccgcag cgtacaaccg gattagcaac ccgcccgaag
    11581 tagagagttt cggcatgatt gttgttgcgg ccgtgggttt gatggtgaac attgccgccg
    11641 gctttattct ctacgaatcg catcatcata atctcaatct acacggcgca ttcatgcacg
    11701 tgattagtga tgccgtcggt tcggtcggcg cgattgtcgc cgggcttatg atgtatttta
    11761 ccgggtggta tctggccgac ccattgatca gcgtcttcat cggattgctg atcctctact
    11821 cgtcctggaa tttgattcga gaggcattga gtatcctcat gcagtccgtc cccaaaacca
    11881 ttcgactcga agaagtccag cgcaccattg agaatgtgaa cggcgttatg gcggttcacg
    11941 atcttcatgt ttgggcggtc acttcgggga tctttacgct cagcgcgcat gccgtcatcg
    12001 gcaatggcca agattttcac gaagtgctaa acggtattga ggaaaccctc aaggagcgtt
    12061 tcagtattga gcacacgaca atccaattgg aaaccgagag ccgggaagaa accgagttca
    12121 agggctttta gtggactgga attgccggga ggatctccgc aagcggaatc taaccttcag
    12181 ctcaagaggc gctcggactc atcacagctg atctgcgtgc cggcagcaac agcgccatca
    12241 gccctacgct gatcaacgaa agtaagccaa gcatccagaa aatcgtcggc agttcagcag
    12301 tgaccaaaag caagccgacc accgacgtcg aaatcgtttt tgacgcctgc tggatcaagc
    12361 ggtcgaaaga cacgatgccg ccgcgcattt cgagcggcgc attttgtgtg agcaggcttt
    12421 tttgcattgg cgatatcacg ccgttggcaa taccgtaaaa aaaaagcgcg cccccgacca
    12481 accaaacgcc gggcataaac ggcacggcga ccaaagcgat gccactgatc aagaaggcga
    12541 taaacagtac tcgggcctta tccctgcctg ccgccagtcg ccccgcctga ctcgaagtaa
    12601 tcatcgcgcc cgcagaaaaa aacacgtaca acaagccggc cgttgccgtc gagatgccgt
    12661 gggtgcgcac gagaaaaagg ggaaagtaag tcaggaatcc atagtcgagg aagaagcgca
    12721 agaatcccgc cgagaacgcg acgagcaagc gggggtggcg cgttaatttc agcatctcgc
    12781 ccagataggc gcgggtgccg ctggtcacgc tgcctttggt ttcgggcatc cagaaaaatg
    12841 tcatgatccc caagggcacg gtgatgatgt aaagagtgaa aggccagtac caggcaagcg
    12901 cggccaacag cccgcccagc ggcggtaaaa tcatatagcc gacacgatcg agaaagacct
    12961 tcagcccttg accggcgatc tcctcgtcgc catcgagcaa atcaccgatc agcacaatcg
    13021 tcaggggaat gaccgcgctg aagccgagtc cctgaatcgc gcggaagacc agcagccaag
    13081 tgaagctggt cgcaaatgcc atggccgtgc cactgatgcc gaatagtata aggccgccgg
    13141 cgagaagcag ccttctgccg tacatatcgg cgatcactcc aaagatgggc gacagcacga
    13201 tggccggcgc ggtgaagaca gtcatgatta acgaaagagc cgaatcactg atgccgaagg
    13261 gttcggtcat tgccggcaag gcgggttgga tgaaattgac gcccatcatc agtgccaagc
    13321 ttgcggcata aaccaggctt aacgagcgcc gcttctccgg tgccagcacg cccaaaccaa
    13381 aaaaaacggc gatggcagac atccaccgca cgctagacca atgcggggag tgagtcaaat
    13441 gtatctgccc gatgtgacga tggaaggtgt gaagagccgg gatgagcgcg gctcaatttt
    13501 gcgatgcgaa aagcttctgc agtcttttta gcgcgttctc cttctcgctt ctgtcgattc
    13561 gcactcgctc cagtgtgcgg cgcagaaatt caaccgaatc atcgtacagt ttgcaattga
    13621 ccggataggg atgcccgtcc ttgccgccat gcgcgaagct aaattttact ggatccttcc
    13681 aagatgcggg ggtgccgtag gcaagatcag cgatgagagt gagggcgcgc aaggttttgg
    13741 cgccgactcc gggcatgctc aataattctt cgaagtccgt ggctttgcgt tcgtaggtct
    13801 tcagcagaat cttttcgatg gaattcggat gaatgtcgcg gacgcaaact tgatgatgcg
    13861 aaggcaacga taacgtttgc agacgatgaa tttcaccgat gagcttttcc ggccgctcat
    13921 gcgagagatc ggtaatcatc gactgcgctt tctggctttc catagcaacc agattgagcg
    13981 cacgctgttt ttgcaggttg ctgcagattg cggagtgcgg ctcgagaacc atggaccgga
    14041 cgctttcgga aaaccagtga tagcgtcgcg caaaacgatc gttcatgcct tgttgcacca
    14101 cggcccaggc gccgttgaaa gtgaaaaaga agctgtgatg gtaaagctga aaaccatcct
    14161 gaagcgcgtt gttatccact ttggccgtga gccggctcag ataaaccaag gcgtcgggac
    14221 tgcagttcag agaatatttt tcggcggccg tgacaatctc ggcgggagtt tttcgggaag
    14281 tcgcgccctt accgccggcg atgaccaagc cgagttcctt ctctctgcct ttaaggcctt
    14341 ccttgagcgc gccacacacc gtcgtcgtga cgccggaaga gtgccagtca aagccgagaa
    14401 cacaacccag ggattgcaac caaaaaggat cggctatgtg ttgaagaaat gcttcaggtc
    14461 cctcgttttc aatgatgagg cgggtgattt cgcggccgag cctgaccatc ctttgaaata
    14521 gccatgcagg cgctttgccg ccgtggagcg gcagttgagc aatcccggtt tgtcgcatgg
    14581 attattcatc ggtctcgcca aagtagtctc ttagaattcg ctcttttatg taagcgattg
    14641 cgccaagatc ttgaatgatg tcgccgccag tatcgtagaa gaaaatgtcg ccctttttgt
    14701 tacgcccaat cgcgacgagc cattcgagtt cgtcacggtc ttccaatagg taattgatcg
    14761 cctggttcgc ccctttgctg gcgatgatag ttactttagg gtttgcggat ttcttcggtt
    14821 gggtcatgaa ggagcctcct gaacgccatg agtttatacc agaagatgag gccgtacaac
    14881 ttacgttgaa gaaacgccgt cgattcgttc tcgggcaagt ctcccacgtc taaggatcag
    14941 aggaaaatat caccgaagcg aaaaatcgac ccgattgggt ttgcccttag gcatggcttt
    15001 ctcttcgttg gtgggcggct tggccgcgac aatcgacagc cggtctgcgc ttacctgtcc
    15061 ggttgccacc aaagcatcgc gcactgtttg agcccgttga ccggctagtt cacgaagatc
    15121 ttcctcgccg acggtggcgt gctgaatcat tagcttttcc atttcgacca cgggtaaatc
    15181 gcgcagcagc ccgatggcat ttcgcggctt tgggaaactc tcctgccggt aggcttgtct
    15241 cagatattgc ggatactcgc tgctttcgat gcggacctcg tcaaccgatc taggcgcttc
    15301 gctcttgcct gatagctctt ttagcttctg cgccttgact ttccgttcca tggacacgcg
    15361 tttgagcccg tctagatcgg cggcgggatc ggcgcgaccg ctaagctcga gtcgtaaggc
    15421 cggacgatcg ttcatcgctt tggccaaggt ctcaatcttt ttttgggcag tctggctaag
    15481 ggtggcgcgg ccgctatcga attcaacgta gctcagttct tcgccgccac cgctcccgcc
    15541 gccgaatgct gagccgagaa gcgcaaacgg cgcagtcact gctcgggtga tgatgttaat
    15601 caccagacgc agcacgagac cgccaacact gaactgggga tcatcgagag agccgctgat
    15661 cgggaggtcg acgtcgatga cgccgtttcg atctttgagc aaggcgaccg ccagcaacac
    15721 cggcaacttg gtggcctgcg ggctctcgac tttttctccg aacgtcagtt ggttaagaat
    15781 gattttgttc tgggcggtta atttacggtt ttcgacctta tatttgacat tgaacgagag
    15841 cttgcccgat tcgattccgt agccgacgta tttcccggag tacggagaga acgggttcat
    15901 ctcgatctcg cgagcgtcgg ccacaatgtc caagtacagc tcttcgccca gaggattgat
    15961 tttgccgtcg atatcgaccg gggcagcccc atccaacttg gcttgcaggt cgatatcgcc
    16021 cggagcttcc ggtttgagct cggagatcgt cccgtgcacg tcggttagat tagccgaata
    16081 gttgggtttg atcaggaagt cgctgaaatt gatattgccg ccgtgaagat taattttgcc
    16141 gatagtgaac ggcttcgccg tatctggcgc gtcggtgccg ggtgcagcga ctgtttgtgt
    16201 gggcggggcg ttttcttggt tgtcgtcctt agacgccttt aagttctgca aattgatctt
    16261 gccatccggc ccgatgatca cgcgcgagta gaaatcggtg acgtcgactt cgccgatccg
    16321 cacctgtagc ggttctgagg tcaattcgaa ttgatgcagt ccgagatttt tccatttcac
    16381 cagatcctgt ttattgtctt tttctatgct ggcgaagtcg tagagctcta tcccgccctg
    16441 gtaatttacc tttgccggac cggtgccgcc atcaactagc gtaaagctgc ccttcgtgct
    16501 gattcggccg ccggttagaa gaaaattgac ttgatcgccg aagtacggct ggagagagag
    16561 agcatcgata tcgtaggcct cgacggcgag tttcgccgag accggatttg tggccgcggt
    16621 accgttcacc cgcaatgccc catttttgtt gatacgagtc cgcagcctgg tcttaccggg
    16681 ctgatttttc gcattggaaa agttgtcgat tcgaacgaaa agatccgata acgtgaactt
    16741 cgctggagta gcggcaccgc gatcgtcgac atcaaccttg aaacgatcca gagcaatttg
    16801 tttggcttca atcttccact cggtctcgtc tttttttacc ggctctttaa caggcgccga
    16861 tgattgggct ttgacgatgc gcgcatagtt cagagtgcca tctttatccc gctgcacgtg
    16921 cccgatgcca tctttgcctt ggatcgcgcc gatgacgact gttttacgta agaggtcgac
    16981 gctaccgtct ttgatgatca atgatggaat gcgccaaagc ggttccggtt gtccaggcag
    17041 atccaggcgc accgttttca aagtagcatt gagatccgtg agttttaggt cggatcctgc
    17101 gtctttggta tcgaatctgt attgtgtcgt gagatctaat aaagcttcct tgacctccag
    17161 ggcgaccgcg ttctgataat acgggctcac gtttcctggg ttgaagcctt cgatctcgag
    17221 tttcccttcc gcgagaaacg gttctaactg gatcttgcca ttttgactga tccgctcttt
    17281 ggcattcgat tcgaaagaaa cttcgagatc ggcttgcttc gccggttcgt tggtcaatcc
    17341 tttaagggca atacgaacat tatcgagtcg ggttttgtag ggccgctgca aagtttcatc
    17401 catgaagtgc agtgtgcttg agtcgataag aatgtcgtca acttcgtaga ccaacggttt
    17461 gctgtccttc ttgggttcga ctgaactcgt cttaggggag atcgtaacca gatcggcagt
    17521 attcaaagat ccatcgcgat tgcgcgctag gtgcaactcc aggccatgag ctttgatcga
    17581 ttttaagcga gtcttgttgt tgaatacatc gatcgaggca atcgcaatgt cgaggttggc
    17641 catcttgccg aatggcgtgc ccccgttttt ctccacggca aggtccgtta gcgccaggtt
    17701 gccggtgatc gtgaatacgg atgggctgtc cttggcgctc ttgaacgagg cattgagctt
    17761 gccgtttagt tcgcccgaag gaactttgaa attcagagct acgggcgaat attcgagata
    17821 cttgttgatc tccaatttgt cgatgttcaa atcgactgtg ctttcgtgag agctgtgaaa
    17881 tggcttggag gccccgacga tatgcaaagg agccccattg atatctgccg aaaacgaggg
    17941 cttgacgaag atatccgtat aggacggcaa acttgaaata aacggcacgc cgatattgat
    18001 cgatgatatc acgtgtttgg tctgttccgg ccggtcgtca aattcgatcc gaccgtcgat
    18061 taactggatg ttatttaagg caaacttcgg cgtcggacca ccggatggtc cggaggttaa
    18121 ctcatcgatg atgtcttgaa agttgtactt caaatcctcg ttgcgcacta gattgacata
    18181 aggctttacc agacgaagct ctttgatgac ggggctcagg cggaaaattg actggagttc
    18241 caaattgacg tgcagctcat cgaaggatac tgccgtcgct tggctctcac gctctttcat
    18301 cagaaacccg cgaacggttg ccgtcatcgc atagggattc aaacggatct gctcgatgct
    18361 gacttcgcgg tgaagttttt gcgacaggtc gcgggcgatt ttgccgcgca gcagcggcgg
    18421 cgctaccact cccaacagga cgccaaaggc gacgacgaga gagaccgtcc aaattgctat
    18481 tttgcgggca cgcggatgga cggtgaaatc tcgcgcacga ctgaagatct gtcttgcgtt
    18541 aactggcatg ggtacaaatg cattggaaaa ctaacgagat attactctag caagtaacgc
    18601 ttgtccacga agagggcgcg cacaatgttg ttcgaacagg caatgttgcg ggaggttcca
    18661 tgagacgtca agattcggca cgaacttatc ctttcgcctg gaacttgaag gctgagtgga
    18721 ttatttttcc gccttgattc cctcgagtac cgaaagatag aggctgggga gcttccaatg
    18781 cctcgcgcct tcgagaatta atcgtttgta ttcggcgttg ggcaagggaa catcgagctc
    18841 caattcggca atataaacca gtgcgtgtag cggtgccttt tccccgttta agccgaaaac
    18901 cggcaaaacc tcacgtcgat agccgtcttc gaagctatcc aagatgagca agtcctcatc
    18961 gcaaatgtcg tatacggcgc cccaaacttc cttaccccgc gctgcaatca cgttggccgt
    19021 gccgcagctg cgcaggcgcg aatggcgtgt gatgccgaat tgatagtccg gcaatcgggc
    19081 gatgcaaacg aaccgtgacg acgggcaacg acgttgcatc tgtggccagt tcaggttcga
    19141 gccgtaggca aaataataca cgaactagtc ccccttatct tttgacaaca acaattatgt
    19201 agtgaacaga aaagttgtca atgttcacat acaatcagac ttagcaggag ttaatccagt
    19261 tcgccttagt cctcttctcg ttttatcggc ggtttggcaa acagaattag aaaggcagag
    19321 gtgaataagg cgcaggagaa gattgtgaag gatatgttgt aactgcccat gcgatcatga
    19381 agaaagccga aaaatggcgc gccgctggcc gcgaatagat gagaaaagaa aagcgacaga
    19441 ccgcgcactt ttccgagaga cactcggcca aagaaattag cccagatcac ttcacgcaaa
    19501 acaaagcttc ccgccaatcc ggtgccgtag agaaagaacc ccaagtaaac gaaacggata
    19561 tcaatgctgg caatcaccgt caccagccca gctccctgga tgagaaactg gatagaggat
    19621 acttttctaa ggtcgaattt gtcggccagc acaccccaga caagcgtaga gccgagttgg
    19681 gttagcgcaa tagtgctcat aaacgaagcc gcgatcagcg gcgagtagcc gatatcggtg
    19741 acataagaaa agatgtgcag attgagaccg gcaatgccga cgctaacgat accgaaagtc
    19801 actaccagga gccagaagac tggcaaacgt aagacatccg accggttcca tacgatttca
    19861 ccgtaggctc ttcttggttg cgcattcgat gagcgctggc cagttggcgg tactgcttca
    19921 aacctctccc gcggcggctc tccatcaggc cgaaggccca tatcttcggg tcgccggcgg
    19981 atgaagatca gcgccggcgc gaccactaaa agaggcgcga cggtgccgaa caccgcccag
    20041 gtgccgcgcc aaccgatgag gacaaacaac catgctgcta tgagcggtat cgcaagcttc
    20101 gcaatgcccg ttccgagatt ggcaatgccc atggcccggc cccgcttgcg gacgaaccat
    20161 tgcgcaatgg tgacgttgat gaccaacgaa cccagtaaag cttcgccggc gattaccagc
    20221 gaacagcgta ccaggacgaa ttgccaaaaa gtttgcacga tgctcgaaag caacaagccg
    20281 gcaccttcca tcagtactcc gatgacgatg atgccacggc cgccgtgccg atcgacccaa
    20341 ggcccgagaa gcgggacgat gatcgccgcg atgccgatct cgaaagtgcg gatcagcgaa
    20401 aatgcgccgc gcgaaacgcc taactccgca gtgatcggct tgagaaaaat gctcaaggtg
    20461 ctcgaaaagg caaaggtccc ggcgatgttg accagaattg cgaccgcgac gatgtgccaa
    20521 ccgtaaaaaa atcgcggccg tttcgacttt agagctgtgg tcatgtcagg cgagttacgg
    20581 gttgccgccg gttgaagggt ctgggaagaa ctacttgaat agaatactcg aatagagacg
    20641 gtgcgtgaag gaagcgcacc ttggtgaatg tgcctatatc taaatcatcg atacggcgtc
    20701 cgatatgaca acagcaatcg cctgctgtca accgtgcgcg cctaaaatcc gcagttgaaa
    20761 aaatcgccgc atggcaataa ctatggcagc gcagcccatg gaggtagcca aatgctgatc
    20821 ttatcgaatg aagatattga aaagattctg ccggtgggcg cctgtctcga agtgctggaa
    20881 gaagcctacc gcgatctggg cacaggcatg gcggcgaccg tgccgcgcta cgatgttttc
    20941 agtccgacca agcccagtga attttacgaa tacaaaacca tgagcggggt tctcccgaac
    21001 cgcaaaatcg cggcattgcg cctcaattcg agcgtggtta cgtggtacga aaaggccggc
    21061 ggtttgcgaa aagacaagct tccggttgcc ggcggcgatc gttacgttgg cttagtcatg
    21121 cttttcagca ctgagaccgg tgagcctcta gcaatctttc ccgatggtta tgtgcagaaa
    21181 ctccgggtcg cgggtgccag tgccatcgcc gcacgccacc ttgcgcgcaa gaatgcatct
    21241 gtgatggcgc tgctgggaag tggttggcaa gccagcgctc atctaattgc gcttagcttg
    21301 gtgcgtgatc tgaagaaagt catagtcttt agccccaacg cggagtcccg aaatcatttc
    21361 ctggacgagt ggagcgacaa agtttccacg gaactcgtcg cagccgttag cgcggccgag
    21421 gccatcgata gcgcagatat cattacctgt gcgacgaatt caatttccgc tctgtttccc
    21481 ggcgagttgc tcaagccggg agtccatgtt tcctgcgtta agccttgcga gctggatgcg
    21541 acgacctacc ggcgatcgga tccgttgatt atccactgga aggaggccaa gccgtttcag
    21601 atcgtcatcg gcggcgtcga tccgcaatcg attccggatg tcgctgacgg ttggccgcat
    21661 ccccttacgc gggaggatac tgctgtgtgg gacttgccga cgatttccca attggtaaac
    21721 ggacagcacc caggaaggac caaagacgag gcgattacct gcttttgtaa caacgtgggc
    21781 ctcggcttgc agttcgccgc ggttggcagc gaagtgttgg cgcgcgcccg agaagcccga
    21841 gtagggcggg agattccgac cggctggttt ttggaatcgg ttcacccgta atgcccatga
    21901 agtttgagca gcacgtgtga gatgttctcg ccggcgtata cacgccggtt atctggcgcg
    21961 gcgcagctta ccgataaaat gttgacaggg tagctcagaa ccaatccgga gccgatccgc
    22021 aacgaggtgt ttgtgaaaat caaaatagca ttgcttacat tgctgctagt ctttttcgct
    22081 acggcctggg ccgcgatggc acagaccctt aaacttaacg ttgcttatcc caccactgtc
    22141 ggttcgatgg cggtgctgtg ggtcaccaaa gaagcgcggc ttttcgagaa gcacggacta
    22201 caagtcgagc tgatctatat cggcggcagc tccaaagtgg ttcaagcaat cctggctaga
    22261 gagatcccaa ttgccgaaat tgcaattcca gcagtggttc aagccaatct ggcaggcgct
    22321 gatctggtga tgcttgccgg gcctaatcac aagccggggc agaaaatcat ggttacgccg
    22381 gagataaaaa aaccggaaga gctgaaaggt aaaaaaatcg gtgtgacgcg gttcgggaca
    22441 tcggacgatt ttcttctgcg ttacgtattg gggcaatggg ggattgcgcc cgaccgggag
    22501 gttgctttaa tccagatggg cggttcgcaa gagacattgg ccggaatgat cgccaaaagt
    22561 gttgatgggg gattactatc gtcgccgcta catcttcggg ccggcaagct ggggttttcc
    22621 atgttggcgg atctgagcgc catcggtgtt gactatcagg gcgccggtgt ggtcaccacg
    22681 caccgctatc tccgcgaaaa ccccgatacc atccatcgct acttgcgagc ctatgtcgaa
    22741 ggtttgcacc ggttaaagac cgataaagca ttttcgatga aggtgattgc caagtattcg
    22801 cggattagcg aagccgctgc cttagaggaa acctatcagc attatgcggt taaagtgatg
    22861 ccccgggtgc cgtatccaac cacgaaaggc atccagatgg tgctcgacga gatgagcgca
    22921 cgaaatccca aggctaagag tcttcagccc ggcagcttta tcgatgtagc aaatttgaag
    22981 gagctagagc agagcggttt catcaagcgt ctctatggcg agtgatttct gctggttcat
    23041 ccatgatcga gaatttcaat atcagatcgc cgtttacaag cgtcgtcaaa ttcacagtca
    23101 atccatagcc gagtggcata gcggatcgtc gaagcgacca gttttcctct tgttgcaaat
    23161 gcgaaaactt cgcaaactcc agacttccac gaacttgttg gaaaaattta aactaaccat
    23221 cgagaaacac caaatgttgt ctccgggtca gagaatctta gccaccgtct cgggagggcc
    23281 ggattctgtg gcgatgcttc atcttctatg cgatttgcgc gaggaacttg agcttacgat
    23341 cgaagttgcc catctacagc atgggatccg cggcgaagaa gcgctcgagg acgcgcgttt
    23401 tgttgctcgg ttggcggagc aactgggttc gatctgccat ttaaaggaag ttgatctgcc
    23461 gcgactgaaa gtcgcttccg gtaaaactag cctcgaagag atcggccgat tcgaaaggta
    23521 ccgctttttt gccgaaaccg cccatcgacg tggactcgat gcggtcgcaa cggctcatac
    23581 cgctgacgat caagcggaaa cgtttgtcat gcgcctattc cgtggcgcag gtcgaaccgg
    23641 ccttcgtggc atcatgccgg tgggtcaact ggccattggg cttgagcgcc cttgcgcaaa
    23701 tgtgctgctg attcggccct tgctcgatac ccgccgaaga gaggtgatgg aatatctcga
    23761 ccagcgaaac ctgacgtatc gcaccgatag cagcaataag gatctgttct ttttgcgcaa
    23821 ttggattcgt ctaaagctca tgccccaagt gagcgagaga ttcggccata aactgaccga
    23881 acggctttgt gctcaagcgc aggtgctgcg cgaggaggag gattaccttg ccgaggtgac
    23941 ccggcagcag ttcgaaaacg tgcgcgatgg acagagacta aaccgtaagc tctttctcaa
    24001 tctcaacagg gcgctgcaac gaagagtgat tcgactctgg atcgcgcagc ggcgcggcca
    24061 tttgcgcgca atcgacttcg atcatatcga agcggcccag tcgttgatcg ccgacggtcc
    24121 gccgcagggt cgtctggctc ttcccggcgg ctggcagttg gcgcgccaat acgaaaccat
    24181 cacttttgaa agaggtcgtc cgaatattaa accgcaatgt tatagctacc tctttaaacc
    24241 tggaatcagt ttacctgtgg tagaggcagg gatcaggatc gaaagcagct tgatagatcc
    24301 agcgccgggg caattgccgg ataactccat ggaagcgctg ttcgacgccg atctcttaaa
    24361 gggagagctg atagtgcgaa atttccgcaa tggtgatcga tttcagccct tgggaatggg
    24421 cggacataaa aaggtcaaag acttattcat tgccaatcaa ttgccgttgc atacacgagc
    24481 gtcattacct ttgctcgtga tggataacga aatactatgg atacccggat acggccggag
    24541 tgatttcgcc cggatcggat cgacgaccaa gatttctctg catttgaagg caattcctca
    24601 acctaagtag gaaaactcta tattgatcta actggttgcg tgtgttagtc ttttctggtt
    24661 cttgaaatcg gaggattggg gttgagtcaa tcatcaaaac atatcgctct ctggttggtc
    24721 ctgctattgg ttcttctcgg catcttcagc gtgtttagta agcaggccgg ccgcgatcct
    24781 gaagtcgttt ttagtgaatt cctgctggct gtggaacgtg gagaagtgca agaagtcgtt
    24841 attcagggtc acaacatcca aggcaaatac aaaaatggcg agcgcttccg cactttcgcc
    24901 cctgacgacc ctgaactggt caagagtctg cgtgacaaga aagttaagat cgccgcaaaa
    24961 cccgaggacg attcgccctg gtattacgtg ctgctgatca actggtttcc gatgctactg
    25021 ctgattggtg tctgggtctt ctttatgcgc cagatgcagg tcgggggcgg caaggcgatg
    25081 tcgtttggca agagccgcgc gaaactgctc acggagaatc aacatcgggt tacgttttcg
    25141 gatgtagcgg gcgcagaaga agccaaggac gatctccagg aaatcattgc atttttaaaa
    25201 gatccgaaaa agtttaccag gctcggcggc cgcattccca aaggttgctt gcttgtcggt
    25261 ccaccaggaa ctggcaagac actgctggcg cgggcaatcg ctggcgaggc cggcgtgccg
    25321 ttttttagca tcagcggctc ggactttgtt gagatgttcg tcggtgtcgg cgcgtcacgg
    25381 gtacgcgatc tatttgtcca gggtaagaaa aacgcaccgt gtataatctt catcgatgaa
    25441 atcgacgcgg tgggacggca tcggggcgcc ggcctcggcg gtggccatga tgaacgcgag
    25501 caaaccctca atcagctatt ggtggaaatg gacggtttcg aagctaacga gggtgttatc
    25561 ctgatcgccg ctaccaaccg gcccgatgtt ttggacccgg cgctcttgcg accggggcgg
    25621 ttcgaccgtc gggtggtggt gccgcgtccc gatgtaaaag gccgtgaagg catcttgcag
    25681 gtgcacacac gcaaagttcc cgtcgcatcg gatgtcgaca tcgcggtctt ggcccgggca
    25741 acgccgggat ttgcgggggc cgatctggag aacctggtca atgaagccgc tcttctagct
    25801 gcgcgcaaca acaaagacaa agtagacatg cgcgactttg aactggccaa agataaagta
    25861 atgatggggg ccgagcgccg cagcatgatc atcagcgacg aagaaaagcg cattaccgca
    25921 tttcatgaag cggggcatgc cttggtagcg aagctgcttc cgggcgctga tccgattcac
    25981 aaagtcacga tcattcctcg cggcatggct ttgggcttga cccagcaact gccgatggat
    26041 gagaagcaca cctatccgaa agagtatttg ctcaacaatt tagtcattct attcggtggt
    26101 cgggtcgccg aagagctagt gctgaatcat atgaccaccg gggctggaaa cgatatcgaa
    26161 aaagccactg aactcgcgca ccggatggtc tgcgaatggg gcatgagcga aaaactcggt
    26221 ccgatgactt tcggcaaaaa ggaagaggaa atctttttgg ggcgggattt cacgcaaacc
    26281 gctgactact ctgaaagtac cgccatcgag atcgacagcg aggttcgacg catcatccag
    26341 gagagttatc acaaggccaa agatcttctc aaaacgaatc tggcgctgct tcacaaagtc
    26401 gctgaatcgc ttcttgaacg agaagttttg gacggctcgg aaatcgacgc catcgttaga
    26461 gactttagcg gcaacggcgg cgacccgcta aacccgacac gggcggcggc agttgcttaa
    26521 gtcgggctaa acactctacc ctccgttttt tcatcgaccg atcaggatcg cttcgtgacg
    26581 gcacgtgcga tcctgattct gcttacgtag tcagggattg gcgacaatat tatcggaatt
    26641 aggattctgg caggccgatc ttatagccag gagcgtgccg cgagaagccg cgacggtact
    26701 gaagcgctat ttcaatgcta tttgtgagtg gtcttgtaag gctagtgttt caatgagaaa
    26761 gcctagcgcg atcattacag gagagaatca gagcccggtt gtcgtggcgc gaagcatcaa
    26821 ctgtccttgc ttcgaccagc tcagttgggg tgagcggatt gccgctcgct aggtttttat
    26881 tgatgtgaat tccagcatag accctatcga cattgccgat catcagcccg ggcgagctct
    26941 agccacgcga catggcctat tagatatgac ctgtcacacg gtggtcatgg gaatcgtcaa
    27001 tgtcactccg gactccttct atgacggcgg caaacgattc gacggcgcaa gagcaattgc
    27061 tggtgcgatc gagatgatcg attcgggggc cgagattctc gatattggcg gcgaatctac
    27121 tcggccaggc gctcagcctg tatccgtcga tgaggaacta aggcgcgttc ttccggtgat
    27181 ccgagagctg cggcaaagat ccagcgtgcc gatttccatc gatacttaca aagaagctgt
    27241 tgcgcgcgcc gctttggacg ccggtgccga catcgtcaac gacatcagcg cgctgcgatt
    27301 tgatccggcg atggctgcgc tggttgcccg ttcaggcgtt ccagtcattc tcatgcacat
    27361 gcaaggagtg ccgcaaacca tgcaacgaga gccgcgctat caagatgttg tcctggaggt
    27421 gcaagacttt ttggcggcgc gcgttcgcag tgctagcctc gctggaatcg cccgagaaca
    27481 gatcattatt gatcccggca ttggtttcgg taaaaccttg gtgcacaacc tagcgctact
    27541 caaggattta cattcattga aatcgcttgg ccagccgctg ttgatcggcg tctcacgtaa
    27601 ggcttttatc ggaagaatct taaattcggc agctccggag gagcggctcg aaggcagcct
    27661 ggccgcggcg gtcgcggcga cattgagcgg ggccaacatt gtgcgcgtgc atgatgtgag
    27721 cgaaacggtt cgcgcgcttc gggtgaccga tgcgatccgg ctgggtcgct tgccggattt
    27781 aggtgaacga tgacggagat tctgggtcaa ctgcgctggc aggatgcgtt ggacatcagc
    27841 atcatctggt atttgattta ccgtttgttg cagatgcttc gtggcagccg ggcgatgcag
    27901 atgatgatcg gcctggctgt cgttttaatg gcgtacgttc tgtcccgcgg ccttggtttg
    27961 tttacgctta actggatctt agacaatttc ctggcatcga tcattctcgt tataatcgtt
    28021 atcttccaga gcgatatacg tcgcgccttg actcaagtgg gaacggcacc gattttcggc
    28081 gccgagaaga tggtgcaacg gcgagaggac attatcgaag aagttgccga ggcggttcgc
    28141 agtcttgctt ccaggcgagt cggagcgctc atcgtgctgg agcgtgaggt cggtttgaat
    28201 gagtatatcg agatcggcac ccgattggat gcccgagtca gccgcgagct gatcgagagc
    28261 gttttcttgc caggctcgcc aatccatgat ggcgcgctag tcgttcaaaa acgcctcgtt
    28321 accgcagtac gctgtcttct gccgttgagt gcaaacccta atcttcgcac gactatgggc
    28381 actcgccatc gagccgccat cggcgtgacc gaagagaccg acgccgtggc aattgtggtt
    28441 tccgaacaag agggtaccat cgctttagtg gtgggcggca atctcacgga gagaatcgag
    28501 gcatcgcggc tgcgcaacgc actcgaaggt ctcgtgaaat tatgaaagag atctggcggc
    28561 agatcggctc aacggcatcg agcaatgtcg ggttggcagt gctctcgttc atcatagccc
    28621 tgggcttgtg gattgccggc catcgggata tcgaacgagc cattgaggtg cccgtcgagt
    28681 ttcgcaacat cccctctgac ctgatggtgc tcgataaccg tgtcgattac gtggttctcc
    28741 gactcagtgg tccgcgaacc ctcgtctcca ctcttgatgc cgatgatttg aagctgcaag
    28801 tagaccttta tggaagcaaa tccggctcct tgagctttcc cttgggaccg agctcgttta
    28861 acattcctcg aggcgtaacg gtggcgcgca ttacgccgcc gatcattcac ttgcggctcg
    28921 atccggtgat caaacggcaa ctgccggtga gtatacggct ttctggcaag ccgttgaccg
    28981 gctacaaaat tgaggaaatg gcggttcagc ccgagtcgat ttcagtgcgc ggcccggctg
    29041 acgaaattcg gcgcatgggt ttagtcgaaa ctctgccggt tgatattgaa gggagccgca
    29101 actcaatcaa gcgcacggtc aggctttcta ccgacggaaa accattgtcg tttatgccgg
    29161 agcaagtatc ggtggcgatc acgatcgttg aagaggattc gatcaaggac ttcaagaacg
    29221 tcgagattgg cgcgcgcgga gcgagcggcg attatgtgtt aacacccaag tcggctcagg
    29281 tgcggctatt cgggccgcgc aggatactcg atagactgga gcttggcgcg gatcaggtgt
    29341 atgtggatct cgatggtttg gccacaggcg agcatgcggt cgatttaaaa ttcaatctgc
    29401 cggctgaaat caaagtggtg gagcaaaagc ccgcgcgctt taaagttaga atcaataaat
    29461 cgcatcctta ggtctggcaa ggagtgacat catggctcaa gtctcaaaac aaaagcgacg
    29521 cctgtttggc actgacggag tccgcggtat tgccaatcag gagccgatga catcggagat
    29581 ggctctgaag ttggggcgag ccattgccaa ggtcttgcaa gatcccatcg cccggcccgc
    29641 ccagagttcg ggccgctcaa aatcgccatc cctggctaaa acgctgggcg gcgataccgc
    29701 acaccgctat aagattctta tcggcaagga tactcgcctg tccgggtata tgctggaaac
    29761 cgcgctggct tccgggattt gctcgatggg ggcggacgtc caattggtcg gtcctttgcc
    29821 gactccggcg gtggcgtttt tgacccgcac catgcgcgcg gatgcgggag tggtgatctc
    29881 ggcctctcac aatccttatc aagataacgg aataaaattt ttttccttcg acggtttcaa
    29941 gttgcccgat gaagtcgaag cccgcatgga agagatgatc ttcagtggcg aagccgatcg
    30001 cgagcggccc acggcatcgc agatcggtaa agcctttcgc atttccgatg cagtcggacg
    30061 ttacaacgtg ttcgtcaaga atagttttcc gcgtcatttg actctggagg gactgcgcat
    30121 cgtggtggat tgcggtcacg gcgcggcgta tcgcgttgtt cccgagatct tatcggagct
    30181 gggggcgcag gtgattccga taggcgtgca gccggacggc gaaaatatca atcatcattg
    30241 cggcgctctg taccctgaag cggcgcgcga tgtactgctc cacgagaaag ctgattttgc
    30301 cgtttctctg gacggcgatg ccgaccgcgc catctttatc gatgaagacg gggaggttct
    30361 cgacggcgat caagttctgg cgatttgcgc taaagatatg caggagaagg gaacccttaa
    30421 agaccgcacg gtggtggcca cggtgatgag caatctcggg ctcgatcttg ccctcaaatc
    30481 gattggggtg aatctcgagc gaaccgaggt cggagaccga tacgtggtcg agcgcatgct
    30541 cgaacgcggc tacaatctcg gcggcgagca gtccggtcac attctgtttt tggatcacaa
    30601 taccaccggt gacggcgcca ttacctgttt gcaggtgctg gcgctcatgg tcgatacagg
    30661 aaagcgtctg ggcgagctca agcgtatttt tacgcgcctg ccgcaaattc ttctgaacgt
    30721 ccgggttacg gaacggaagg aatttcaggc tatgccgaaa gtgagacggg cgattgcctc
    30781 cgtcgagcaa tcattgaacg gccgcggccg caccctggtt cgttattccg gcacggagat
    30841 gttggcccgg atcatgttgg aaggcgaaga tgaagatagg atttgcgcca tggcgcagga
    30901 aatcgccgat gaaatacgca cggaaatagg agtgaaggat tgatccgctt gggagtgaat
    30961 gtcgaccacg ttgcgaccgt gcgccaggcg cgtcgagtgg atgtgccgga tcccctcgaa
    31021 gccgctttga tcgccgaaaa ggccggagcc gacggtatta ccgtgcacct gcgcgaagat
    31081 cggcgtcata ttcaagagcg agatgtgcag ctcctgcgcg agcggttgaa caccaagctc
    31141 aatctggaaa tggccgtgac tccggcgatg cttatctttg ctgaaaatgt ccgtcccaac
    31201 gacgcttgct tcgtgccgga aaagcgggag gaacttacca ccgaaggcgg tttggacgtc
    31261 gtctctcatc gacaaaaaat tcgcgaggcg acgcagcggc tgcagggcat cggcgtgcgc
    31321 gtgagtttat tcatcgatcc cgaatcggct cagattgagg ccagccgcga cgccggcgcg
    31381 catgcggttg aaattcatac cggcgcttat tgcaatgcgt tgggtatgga gcgcgagaag
    31441 cagctgcaag tgatccgcga agccggaaag ttggcgcaga gtctcggcct ggaggttcac
    31501 ggcggccatg gcctcaatta cgacaatgtt ttggctatcg cccagatccc tgagatggcc
    31561 gagcttaaca tcggtcacag tatcatcgct cgagcgatca tcgtcggtat cgagcaagcc
    31621 acccgcgaga tgaaggatct gcttagcaag gcgaaagaat aaacaggttc aaaccgttca
    31681 agcagttcaa gccgttcaaa tcgtttagat ccggcggctt caacccttag aacgtttgaa
    31741 cggctttaac cattgaacca tgatactagt taccgccgag cagatgcgcg agatcgatcg
    31801 attgacgatc caaaagtacg gtactcccag tcttacgctg atggaacgcg ccggcgcggg
    31861 agtcacgcac gcaatcctgc cgcgtttcgc caagccccta agtaaaggtg tcttgatcgt
    31921 cgccggcaaa ggtaacaatg gcggagacgg ttttgttgtc ggacgcttgc tgcggcagca
    31981 gggggtgttc tgcgaaattg ccctgttggc caagcgcaac gagacttcca gcgacgcagc
    32041 gaagaatcta caggcgtatt tgacagccgg aggcaaggtc gtcgacatcg aggccgatcg
    32101 tctgccattg ctcaatgacg caatcgccgg caaagggttg ctggtcgatg cgattctggg
    32161 taccgggacg aagaatgaaa tccgcggatt gtacgctgag gctatcaggg caatgaacga
    32221 gtccggacta cccattgtcg ccatcgacat accttccggg ctggatgcgg ataaagggac
    32281 gcagttgggg gtgtgcgttc aagctgctat gacggtcgcg ctcgcgtatc ccaagatcgg
    32341 cgaggtgatc catccagccg tggaatacgt cggcgagctt gttgttgccg acatcggggt
    32401 cgagccggct gcggtaaccg aggtggcgcc cgctgcagaa cttctgaccc gtgaagaaat
    32461 acgtccgtta gttccagtcc gtaaaccgga ctcgcacaaa ggaacctatg gacacgtcgt
    32521 ggtgatcgcc ggctcgcgcg ggaaaactgg cgcggcgatt ctcgccagcc gcgcatgcat
    32581 gcgcgccggc gcaggattga ctactttggc gacgccgcga tcgcttaacg atgtttttgc
    32641 cggcgccatg atcgaggtga tgaccgaacc gttgcgcgac actgccgagg aggagatcga
    32701 atcgctaact gacgaggagt ggtggcgact gttggaacgc aagacggtgg cgctgtttgg
    32761 tcccgggatc ggcgttactg actctacgag aaataatctg cgctggttgt tgcgccattt
    32821 gagtattcca tgggtgatcg acgccgatgg tttgaataac cttgcgctgg atatcgaccg
    32881 gctgcgcaag gccaggacag cgcctatctt gacgcctcat cccggtgaaa tggcgcggct
    32941 cctgggcaaa gacgcagtga ccgtcaacaa ggaccgcatc ggcgtagcgc gcgcgtttgc
    33001 ggccgaacac cgatgctatg tagtgcttaa aggcgcgcgc acagtgattt cgacacctgg
    33061 cggcaaaatc ttcatcaatc cgaccggcaa tcctgggatg gccagcggtg gcctggggga
    33121 cgttctcgcg ggcatcttgg cagcgctcct ggcccaaggg ctctctccgg aagcggccat
    33181 gaagttggcg gtttatctgc atggttttat cgctgaccga attgcggcgg agcgaggaat
    33241 gctgggcatg attgcttccg atatcattga tggattaccc gctggccttc gcagtctgtc
    33301 ggctgtcgtt tagactcgaa atttccagca aaatatacta cttgtgttta tggagcggtg
    33361 ttgctgatgc agtgggcggt tatttcgcgc tccgccgagc acacgcgacg tttgggagtt
    33421 agattaggta agctgttgca gggcggtgaa attgttgggc ttattggcga gctcggaact
    33481 ggcaagacct gttttgtgag aggcttgacg gaggggttgg gggtcagcca agaaacgtgg
    33541 attcgcagcc cgacgtttac acttgtcaat gagtatcacg gtcgcttacc catctatcac
    33601 atcgatcttt accgaatcga tagcacgggc gaactcgaag aactaaatct ccgcgagtat
    33661 ctctactcca acggcgtcag tctgattgag tggttcgagt attttcctgc cggcgaggtt
    33721 gacgatcatc tggcaattca attggcgtac cgcgagggca gcaaacgtca gctgacgttt
    33781 gtacctcctg gggcgcg
//
LOCUS       DASUVS010000398        16750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1047365, whole genome shotgun sequence.
ACCESSION   DASUVS010000398 DASUVS010000000
VERSION     DASUVS010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 16750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16750
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047365"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VF182_21345"
     CDS             complement(<1..443)
                     /locus_tag="VF182_21345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX6439676.1"
                     /translation="MLNLIKKIVGTKNDREIKRIRPYVEKINSIEAQLQSLSDDELLA
                     KTDNFKDRIQKATAQLKADLEEARAQALTSDGERREELKGRVEELDKELREGEAAVLE
                     EILPEAFATVRESSRRTIGLRHFDVQLIGGTVLHEGKIAEMKTGEG"
     gene            complement(551..1351)
                     /gene="murI"
                     /locus_tag="VF182_21350"
     CDS             complement(551..1351)
                     /gene="murI"
                     /locus_tag="VF182_21350"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX6439677.1"
                     /translation="MTKRNDAIGVFDSGIGGLTVLQKLIEILPAENTLYLGDTARAPY
                     GTKSVETVIRYSVENTEFLIEKGVKLVVVACNTSTALALEALREKVKVPLIGVIEPGV
                     RKALMISRNKRVGVIGTEATIQSRAYTKALKRRSGDIEVYSRACPLFVPLVEEGWIDN
                     GIAAMTVEAYLGSLKQSGIDTLILGCTHYPLLKKAIGNYMGAAVELIDSAEEIVKEVG
                     MTLEKQSLARRDGIGNHSFFVTDAPDRFIKVGGRFLGEKVESAVRIER"
     gene            complement(1361..2455)
                     /locus_tag="VF182_21355"
     CDS             complement(1361..2455)
                     /locus_tag="VF182_21355"
                     /inference="COORDINATES: protein motif:HMM:NF045247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6439678.1"
                     /translation="MLKFEPQEIFIEQDCQSMPMVSRLIAALPHVPLRIVDPGREEVT
                     FPVPPGTDFQTAKKRLYVAKHKGEFLKKCPGSDGQICCNYFVINFASNCPMDCSYCYL
                     QEYLAHNPALKVFANVDDLLAEAGELLYKHRRYFFRIGTGEITDSLILDPYIGFTREV
                     VPFFAEQPNALLELKTKSNCVEDLLRVDPKERVVVSWSMNPQRVIDAEEHGTASLLER
                     LQAAHLCQDAGYRLGFHFDPMIEYPDWERDYEALVEQLFATVDWRRISWLSMGVLRNT
                     PGLKRAMRERRPRTRLLAAEQVPNPDGKLRYFQPLRIEMYRKMLRWIRRNAPTVKIYL
                     CMESKHVWEQVFGYAPSCEKELGAQLDPHV"
     gene            complement(2449..3024)
                     /locus_tag="VF182_21360"
     CDS             complement(2449..3024)
                     /locus_tag="VF182_21360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439679.1"
                     /translation="MTAQTIQEYALSKGFHPQIKERWLSWNATDGAALLELALALKAG
                     ENHLRDLMDWLEEIELRDGVAISELLQSKTIADVRTDPRLGRADRLKRLKDQVRRIRF
                     PRLSQIEDSIQAKIRQLKLQPAVKLSVPAGLEGGDLQIEFAARSPAEFKSILANLGAV
                     AESENLAEIYLLLKGAENPEMRAAPGASSAC"
     gene            complement(2951..3844)
                     /locus_tag="VF182_21365"
     CDS             complement(2951..3844)
                     /locus_tag="VF182_21365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6439680.1"
                     /translation="MSVVLPTYNRWPMIAEAVESVLAQTFRDFELVVIDDGSTDNTTR
                     LLSSCDSRLRVLSQPHTGVAAARNAGVARSRGKYIAFLDSDDLWLPAKLAIQTDFMEQ
                     NSQMQICQTEEIWIRDGMRVNPKMRHRKPSGDIFQRSLELCLVSPSAVMMTKELFDLA
                     GGFDESFPVCEDYDLWLRIAVGHPVYLIDSPLVTKRGGHVDQLSRSLWGMDRFRVLAI
                     AKLLRDGLAGEKKQWALDSLRQKTSILAAGAAKRGKLDDARKFQALLSEFLEGAGDDG
                     TNNSGICAEQGISSADKGALA"
     gene            complement(3933..4364)
                     /locus_tag="VF182_21370"
     CDS             complement(3933..4364)
                     /locus_tag="VF182_21370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEX6439681.1"
                     /translation="MKIEGSYTFNAPRDRVWQVLLDPNVMAQCMPGCEALNQVGPDQY
                     EATMKVGVAAVKGTYKGKVAIKDKQPPAHYILSGSGSGGPGFMQGDVVIDLEEANGKT
                     VLKYSTDAQVGGLIASIGQRMLGGVAKMMVDQFFKKMETFM"
     gene            4524..6608
                     /locus_tag="VF182_21375"
     CDS             4524..6608
                     /locus_tag="VF182_21375"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX6439682.1"
                     /translation="MNPPTTLVKLHQMFNRAFTAVLLLGAFCGYPSALLSQAPDPRQS
                     FYTAYSLYSSGAVSQAKEFFQQSLNGAYPLTDYALYYLAVIAFDESDWPSSRQLLMRL
                     RQEYPQSIWFGRAQLQSIKIDIAEKNYPQALASLRSLRTEKNLKPEIAEEALLLEAQS
                     QEIQGDVPQAYSSFQELRTNAPRSRWSAAARKEVGRLREKYPELFGLNTVTAISDEAD
                     QLAREREHGAAEALYKKLLNQDLGPMLRLQNLTKLADLYLSIRKRNEAMPLLEEIARD
                     FRDSPQAPVALYRIGSILWNRNDNSQALGYFTRLIKTYPDGAEADRAQFAAADILESQ
                     GKSHEAMPLYRSLPKKFPNSQVRDDAIWRLAWAQYRGGKLAEANVTFKSLASNAKEER
                     YKTAAQYWQAKTSWQLGAHETANRLYQQIFNQGQESYYQALAAQALAKAGITVQQSRP
                     VKPAAANDLDLQSYPEAAFHLVRSRELAQLKLHALAVSELDEINRIARQNSSLRPLLI
                     DEYTSNHAFARSVALAQQLPGAAGERLFHRYPLAYWETIQNKAREAEIDPYLVVALIR
                     QESLFDPRARSSAAALGLMQLLSTTANRMAKQLGLTPPSNEKLFDPELNVTLGTRYLQ
                     SLLHRYSNNWFKAIAAYNAGEAAVDRWEKDIVTDDIEEFVERIPYLETRQYVKLVLRN
                     HRIYKTLYNQDQ"
     gene            6605..7375
                     /locus_tag="VF182_21380"
     CDS             6605..7375
                     /locus_tag="VF182_21380"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX6439683.1"
                     /translation="MSQAPLSRLAVIGITADSSACEQTGFKTNREGLYSLASRYCDAI
                     ENAGGLPIIIPHLRSRIQIGRLLGRIDGLLISGGGFDIDPAYYSEQRMAKLGKINPQR
                     TFTELESIAFGLDRDLPMLGICGGAQAINVALGGSLYQDIATQLPAAQRHQQEERSDS
                     HGHVVEVSRGTLLYKICGRPRLKVNTTHHQAIRNIGAGLTVNAIAPDGVIEGIESKDH
                     SFVLGLQWHPEVLAPREVIQRKIFSSFVSACRRLHRGI"
     gene            7529..8665
                     /locus_tag="VF182_21385"
     CDS             7529..8665
                     /locus_tag="VF182_21385"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX6439684.1"
                     /translation="MANQVGDVLDKGAAERALFPAGGLPGGGQLEVRMELSADHPDPD
                     IRQVARNVKPYNLQSWHSEWSAAADKNEELAAGFVAEERNVTAHEFYLRAADFYRRAL
                     VYLPESDSRMLPTYVKLRETFQKAWSLVPPPFERAQIPYEGHLLEALFFPGRGGAGAP
                     LPVVYNYGGADGILLRGEDGGAGQYVRRGMHFIDVDGPGHGGALREKKLYATADSERV
                     AKAVIDYLVSRPDVDAERIGLHGSSMGGYSAPRCATAEKRIKAVAVWSGAYSLVDDIF
                     DYYPPIRDRLRWLIGAKNLDEAREKIKEFNLEGRANRIECPLLVGYSHDDRVMDPRGA
                     LKLYESAVNSPSRSMLDGVGHGTKRFDRRTYIADWFMKQLKAGQ"
     gene            complement(8681..9124)
                     /locus_tag="VF182_21390"
     CDS             complement(8681..9124)
                     /locus_tag="VF182_21390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX6439685.1"
                     /translation="MEWKDNLIETRAQIRQLIMKTHRIAVLGIKTEAQAGQPSVYVPQ
                     YLLGAGFEIVPVPVYYPEVTEIFGARVYRRLVDVPGEIDLVDVFRRPQDINGHIDDIL
                     SKKPKAVWFQSGIRNDPAAETLARAGIQVVQDRCLMVEHRSVAAR"
     gene            9313..11571
                     /locus_tag="VF182_21395"
     CDS             9313..11571
                     /locus_tag="VF182_21395"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6439686.1"
                     /translation="MADLKVIGKAITREDGYDKVSGRTIYTADVHLAGLLWGKILRSP
                     YPHARIERIDTYKAQRVPGVKAVITGADVKGCLIGKQIRDMPALCWDKVRFIGDRVAA
                     VAAESIDAAEEALTLIEVDYQELPAVFDPLEAMQPDAPRLHDDIAAYGGAPKDLLALD
                     LPNGLTRLAWRKGDVEQAINQADVVLEHTFRIPARHQGYLEPHAAIVSIDKNDRIQVW
                     VSAKNPFGIRSQMAKALDISEERICVNVVNVGGEFGGKGDATDLPVAYFLAQRSSRPV
                     KLVMNYAEELSAGNPAHPTIITIRSGVNRDGRIVGRSLRAIHTGGAYGGLKANSSLAT
                     WHYVGVPYRVENSHFEFLQIYTNTVPGGYYRSPGAVAMAFALESHTDLIASELGIDPA
                     EFRLKTLLSEGEEDAVGHRLRDVRFRAVLEAALEAAKWRDSKLRANVGRGISLSARHI
                     SGGDTGLVLSAQADGSFMVLSPTIDQGSGTHTILRQLVAQQMNVPVEKVRVVIGNTDT
                     APRDSGVRASRVTYVAGNAMMKACGELRERLLERAAGFLECRPDEIEFSDGSFCLRQD
                     PSQQLTLRRLVSQAKDPLQVSVYEDYPYPDEVSYICAQIAEVEVEPATGAVRVHRMIT
                     AHDVGTVINPLTHQGQIDGATIMGVGQGIMEELIMIDGRIVNNNLGDYKLPTIADIPM
                     LKTVLVHSDGGVGPMNAKPIGEFANNGPPAAIANAIADAVGVRLFELPLTAEKIYSLS
                     KGSQRKIAREIP"
     gene            complement(11776..11985)
                     /locus_tag="VF182_21400"
     CDS             complement(11776..11985)
                     /locus_tag="VF182_21400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439687.1"
                     /translation="MSYLLFVLASLLVLITACSGSGGRSRLDNKTISPAQASEERAQI
                     IERLVPETHLSRGPDSVLWLTQRPW"
     gene            12323..13063
                     /locus_tag="VF182_21405"
     CDS             12323..13063
                     /locus_tag="VF182_21405"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6439688.1"
                     /translation="MLDGKVVIVTGAGQGIGRHAATTFAQQNAKVVIADVNEEKARKT
                     ATELARLTEAIALSVDVRDEESVKRMIDQVVDRFGQIDVHVNNAAIVPHFAWGIPRWP
                     RIGEMPKDFWDRVIQTNLNGTFNGTKHVIPHMEKRQTGHIINLYGGGGITPAGACAYM
                     VSKDGIRTFTRYVAEEVRSSNICIVTFSPRVPIVTEDAPEEAFKRLPGPEILGQGFVL
                     AAQLPMEQSGGCFAYDNGKLVRETTREG"
     gene            13165..14184
                     /locus_tag="VF182_21410"
     CDS             13165..14184
                     /locus_tag="VF182_21410"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6439689.1"
                     /translation="MRVIDADAHVIENESTWDHMLESERGFKPRIVGASDGDASADYW
                     LIDGRLMPRSNVEKSLPEAARDMSDLSIRIAHMNELNIDLQVIYPTIFIFPTARRPEV
                     DLALCRSYNRWMADIVSKEPERFRWVVVPPLLNMDSVAEELKFAKERGACGVYLRGLE
                     ADRRLSDSYFFPLYDVASKLDLPICVHSANGSIEAYDFFLDEPGFCKFKLAVVGAFHS
                     LLVDGIPEKFPKLRIAIVEVSAQWIPYAIHDLARRYQRRGKQLGSRVLEDHRVYVTCQ
                     TDDDLDYILGYAGENNLVIGTDYGHADNASEIEALRKLRTEGKLSARVIDKILGDNPC
                     ALYSL"
     gene            14331..15476
                     /locus_tag="VF182_21415"
     CDS             14331..15476
                     /locus_tag="VF182_21415"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEX6439690.1"
                     /translation="MSDIFDLLLTGGTVIDPRSGTNQKLDIGIIADRIAAIRSNIPPV
                     NARKSLNVEGCIITPGLIDFHVHSYWGVNPYGFNADPICQASGVTTTMDAGSSGPTNF
                     LGFRKLVYEQSHTRMLAFVAMAQHGVLNDPGELENLRFADPEGAAQAVAENRDVAIGI
                     KLRMHRKSVGENSREALRLAVKTGEACRAPVMVHVGDTVISMDEIVDSLRAGDVVTHC
                     YTPQQPSIIDKNGRLLDAVRKAQERGVIFDVGHAGGHFDFDLVRRAMGGGLLPDIISS
                     DLHGRLKQPGFGVVGDLPTVMTKFLALGLSLERIIAACTNNPARAVGWQDRIGSLEVG
                     READIAALEILDDPVMLRDSVGVEKLHRRRIAARWTVRAGELFQARR"
     gene            15550..16581
                     /locus_tag="VF182_21420"
     CDS             15550..16581
                     /locus_tag="VF182_21420"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6439691.1"
                     /translation="MRRAILFLFVIVVATSSRAAAQTPYYQGKTITIITGSQAGDLFD
                     IYARMIATHMGKNIPGNPSILVQNMPGAGHIVAANFVYGVAKPDGLTLLAPNPNLYVD
                     QLIGRTEIKFDWAKFAWIGNASRTTDVLYMRTDAPFKTMEEVRSAKEPPKCGATGTGT
                     TGYLVPRLLDETISAKFNIVTGYKGGNEIDLAVERGELQCRAFSVTAFFGREPFHTWR
                     KTNFVRAIIQLGQKRDNRLLDVPTLNELMDKYKTPDIGRRLALIVTASEIFQRPYMGP
                     PGMRPEHVRILREAFHKTLKDSAFMEEVKSKKLDTEYTSGEEMETLAKEMMSQRPEVI
                     ERLKKVLGN"
BASE COUNT         3830 a         4670 c         4503 g         3747 t
ORIGIN      
        1 ccctcaccgg ttttcatctc ggctattttt ccctcgtgga gcaccgtgcc gccgatcaat
       61 tgaacatcga agtgccgcag cccgatagtc cgtcgcgaag attcacgcac cgtggcaaaa
      121 gcttcgggta agatttcttc gagaaccgcc gcctccccct ctcgcaactc tttgtccagc
      181 tcttcaaccc ggcctttgag ttcttcgcgc cgttcgccgt cggaagttag tgcttgggcg
      241 cgagcttctt ccaaatcggc cttcagctgc gctgttgcct tttggatgcg gtctttgaaa
      301 ttatcagtct tagccagtaa ctcgtcatcg ctaaggctct ggagttgcgc ctcgatacta
      361 ttgatcttct cgacgtaagg acgaatccgt ttaatctccc ggtcgttctt agtgccgact
      421 atttttttga ttaggttcaa catgtttaag actttgcccc caaagatgga cgcggagcgg
      481 tgcggcgctt gctaagggaa tcagtggatc atagcttaca agattcgggt gtaaaacaaa
      541 acgagctcaa tcagcgctcg atacggaccg cagactccac cttttctcca agaaaacgtc
      601 cgccgacttt gatgaagcga tcgggcgcat cagtaacgaa aaagctgtgg ttgccgatgc
      661 cgtctctcct ggcgagagac tgcttctcaa gcgtcattcc tacttccttg acgatctctt
      721 ccgcggaatc aatcagctct accgcagccc ccatataatt accgatagcc ttcttgagca
      781 atgggtagtg tgtgcatccc aatatcaggg tgtcgatgcc gctttgtttt aagctaccca
      841 gataagcttc cacggtcatg gctgcgatac cgttatctat ccagccctct tcgacaaggg
      901 gaacgaataa aggacaggcg cggctgtaga cttcgatatc tcctgagcgc ctcttgagcg
      961 ctttggtgta ggcgcggctt tgaatggtcg cttccgttcc aatgacaccc acccttttat
     1021 tcctgctgat cataagcgcc ttgcgaacac ccggctcgat cacgccgatc agcggaactt
     1081 tcactttttc ccgcaaggct tctaaagcca gtgccgtcga agtgttgcag gcaactacta
     1141 ccagcttcac gcccttttca atcaggaatt cggtattctc gaccgaatac cgaatcaccg
     1201 tctcaacgga cttcgtgccg taaggagctc tcgcggtgtc accgagataa agggtgttct
     1261 cagcgggcag aatctcgatt aatttttgca gcaccgtgag cccgccaatc cctgagtcga
     1321 atactcctat ggcatcgttc cgtttcgtca tgagtcagaa ttatacatgt ggatccaact
     1381 gcgcgcccag ctctttctcg cagctcggcg cgtaaccgaa aacctgttcc cagacgtgtt
     1441 ttgattccat gcaaagataa attttgaccg ttggagcgtt gcggcggatc cagcggagca
     1501 ttttgcgata catctcgatg cgcagcggtt ggaaatagcg cagcttgcca tcaggattgg
     1561 gcacttgctc ggcggcaagc agccgagtcc ggggccgcct ctctctcata gcccgcttga
     1621 gcccgggcgt attgcgcagg actcccatgc tcaaccatga aatgcgccgc caatcgacgg
     1681 tcgcaaatag ttgctcgacc agcgcctcat aatccctttc ccaatccggg tactcaatca
     1741 tggggtcgaa atggaagccg aggcgatagc cggcatcctg gcatagatgc gccgcttgta
     1801 ggcgctcaag cagcgatgcc gtgccgtgct cctcggcatc aatcaccctc tgcggattca
     1861 tcgaccacga aaccacaacg cgctccttgg gatcgaccct caatagatcc tccacgcaat
     1921 tgcttttggt tttgagctcg agcaacgcgt tcggctgttc ggcgaaaaag ggcacgacct
     1981 cgcgtgtaaa tccgatatag ggatccagta tcaggctatc ggtaatctca ccggttccaa
     2041 tacgaaagaa atacctccgg tgcttgtaga gcagttcgcc tgcctccgcg agcaaatcgt
     2101 cgacgttggc aaaaaccttc aacgctgggt tgtgcgccag gtattcttgc aggtagcagt
     2161 agctgcaatc catcggacaa ttgctggcaa aattgatgac gaaataattg cagcagatct
     2221 ggccatcgct gccggggcat ttctttagaa attctccctt gtgtttagcc acgtaaagcc
     2281 gcttctttgc cgtttggaaa tcggttcccg gcggtactgg aaacgtgact tcttcgcgac
     2341 cagggtcaac gattcttagc ggaacgtgcg ggagcgcggc tatcaaacgg ctaaccatcg
     2401 gcatgctttg gcaatcctgc tcgatgaaga tttcctgcgg ctcgaacttc agcatgctga
     2461 tgatgctccg ggggctgcgc gcatttcggg attctcggcg ccttttaaca gaaggtagat
     2521 ttcggccaag ttttcgcttt ctgccaccgc gccgagattt gccaggatgc tcttgaattc
     2581 agccggactt ctcgcggcaa attcaatctg caaatctcca ccctcgagtc ccgccggaac
     2641 cgacaacttt accgcaggct gcagcttgag ctgacgaatc tttgcttgga ttgagtcctc
     2701 gatttggctc aagcgcggga accggattcg tctgacttgg tccttgagcc gcttgagccg
     2761 atcggcgcgg cctaaccgtg ggtcggttcg tacgtcagcg atggtcttac tctgcaaaag
     2821 ttcactgatc gcgacgccgt cgcggagttc gatttcctcc agccagtcca tcaggtcacg
     2881 tagatgattc tcccctgctt tcaaagcgag cgcgagctca agcagtgcgg cgccatcggt
     2941 tgcattccag ctaagccagc gctcctttat ctgcggatga aatcccttgc tcagcgcata
     3001 ttcctgaatt gtttgtgccg tcatcgccgg ctccttctaa gaattctgac agtaatgctt
     3061 ggaacttccg ggcatcgtcg agcttgcccc tttttgccgc gccggcagct agtatcgatg
     3121 tcttttgccg aagtgaatcc agggcccatt gctttttttc gccagccagg ccgtcgcgca
     3181 gcagcttggc gattgccagc actcgaaaac ggtccatgcc ccaaagagaa cgggacagtt
     3241 gatcgacgtg cccgccgcgc ttggtaacca aagggctgtc gatcagataa accggatgtc
     3301 cgaccgcgat acgaagccag agatcataat cctcgcagac tggaaacgat tcgtcgaatc
     3361 cgcctgccaa atcgaaaagt tctttggtca tcatcaccgc cgatggactg accaaacaaa
     3421 gctccagact gcgctgaaaa atgtccccgc tgggctttcg gtgcctcatt ttgggattaa
     3481 ctctcatgcc atcgcgaatc cagatttcct cggtctggca aatctgcatc tgcgaatttt
     3541 gttccatgaa atcagtttga atcgctaact tggcaggcaa ccaaaggtcg tcagaatcga
     3601 ggaacgcgat gtatttcccc cgtgacctcg ccacaccggc gttccgcgcg gcagcaacgc
     3661 cagtgtgcgg ctgagacaat actcgcaatc gagaatcgca tgaactcagt aaccgcgtcg
     3721 tgttatcggt cgatccatca tcaataacga cgagttcgaa gtcgcgaaac gtttgagcca
     3781 gaaccgactc gacggcttca gcgatcatcg gccatcggtt gtaggtcggg aggactacac
     3841 tcaccagagg cgggccggct gtcatggcgt cgtatttgta aatcggagct taagatctca
     3901 cgggccggaa aagtctcgat tgtgctcagc cttcacatga aggtttccat ttttttgaaa
     3961 aactgatcca ccatcatctt agccacccca cccaacatgc gttgaccgat actcgcgatc
     4021 aagccgccga cttgcgcatc ggtgctgtac ttgagaaccg tttttccgtt ggcctcttca
     4081 agatcaataa cgacatcgcc ctgcataaaa cctggaccgc ctgagccgga tccgctcaaa
     4141 atgtaatgag caggaggctg tttgtccttg atcgctacct tacccttgta ggtacctttc
     4201 accgcggcga caccgacttt catggtcgct tcgtactgat ccggtccgac ctgattgagc
     4261 gcctcacaac caggcatgca ttgcgccatg acatttggat cgagtagaac ctgccagacc
     4321 cgatccctcg gagcattgaa tgtatagctt ccctcaatct tcaaaacggc tctccttcgc
     4381 gcttgtttcg ttgcgatgga cctatctcga aataactatt atcgagccga ttaccagcaa
     4441 aaaccttagc tttcatcccg acaaaaaaca acactaacat atccgccgca ggcggcggcg
     4501 ccacgagctg aacagaaaaa tcgttgaatc cccccactac cttggttaaa cttcaccaga
     4561 tgttcaacag agcttttaca gcggtactgc tgctgggggc attctgcggt tatccgtcgg
     4621 cgcttctctc gcaagcaccc gatcctcgtc aaagttttta caccgcctat tccctgtatt
     4681 cgagcggcgc agtctctcaa gcgaaagagt tttttcagca aagcttaaat ggcgcctatc
     4741 cgctcacaga ttatgcgctc tattatctag cagtcattgc atttgacgaa tccgattggc
     4801 cctcgtcgcg gcagctcctg atgaggctgc gccaggagta tcctcaaagc atctggtttg
     4861 gccgggctca gctgcaatcg atcaaaatcg acatcgctga aaaaaattat ccccaagcac
     4921 tggcctcgct gcgttccttg cgaaccgaaa agaacctgaa acctgagatt gccgaagaag
     4981 cgcttttgct tgaggcgcag tcgcaggaaa ttcagggaga tgtccctcag gcctactcgt
     5041 catttcagga acttcgaaca aatgcgccac ggtctcgttg gagcgccgcg gcacgcaaag
     5101 aagttggccg tttgcgcgaa aaatatcccg aattattcgg cctcaacacc gtcactgcga
     5161 tttctgatga agccgatcag ctagcgcgcg agagagagca cggcgcggcg gaggctcttt
     5221 ataagaagct cctcaatcaa gatctaggcc cgatgctacg gctgcagaat ctcaccaagc
     5281 ttgcggatct ttatctctcg atccgcaaaa gaaatgaagc gatgcctctc ctcgaagaaa
     5341 ttgcccgcga ctttcgggat agcccacaag cgcccgttgc cctttatcga atcggcagta
     5401 ttctatggaa ccgcaatgat aacagccaag ctctcggcta cttcacgcgt ctgatcaaaa
     5461 cgtatcccga tggcgcggaa gccgaccgag cccagtttgc cgctgccgat atcttggagt
     5521 ctcaaggcaa gagccatgaa gcgatgccgc tgtaccgttc tttgcccaag aagtttccca
     5581 acagccaggt tcgcgacgat gcaatttggc ggttggcctg ggcgcagtat cgcggcggta
     5641 agttagcgga ggcaaatgta accttcaaat cgcttgcgag caacgctaaa gaagaaagat
     5701 acaagaccgc cgctcagtac tggcaagcaa aaacatcttg gcagcttggg gcgcatgaga
     5761 ccgctaaccg cctctaccag cagattttca atcaaggcca ggaatcctat taccaggctc
     5821 tggcggcgca agcgttggcc aaagctggaa ttaccgtaca gcaaagcagg cccgtcaaac
     5881 cggcagcggc gaacgatttg gatctgcaat cttacccgga agctgctttt catttagttc
     5941 ggtcgcgcga attggcccag cttaaactgc acgctctggc agtgagcgag ttggatgaaa
     6001 taaaccggat tgcccggcag aattcctcac ttcgtccgct cctgatcgat gaatacacga
     6061 gcaatcacgc gtttgctcgg agcgtcgcgc tcgctcagca gcttcccgga gccgccggcg
     6121 agcgcctgtt ccatcgttat cctttggctt actgggaaac aatccaaaac aaagcgcggg
     6181 aagcggaaat tgatccttat ctggtcgtgg cgctgattcg gcaggaaagc cttttcgatc
     6241 cccgcgcccg gtcctcggcc gcagctctcg ggctcatgca gttgctgtcg acgactgcaa
     6301 atcgcatggc caaacaactc gggttgacgc ctccgtcaaa tgaaaagctc ttcgacccgg
     6361 agcttaatgt cacccttgga actcgctatt tacaaagctt attgcaccgc tattcaaaca
     6421 actggtttaa agcaatagcc gcctacaatg ccggcgaagc cgccgtcgat cgctgggaaa
     6481 aagatatcgt caccgatgac attgaagaat tcgtcgagcg cattccgtac ctcgaaacac
     6541 gccagtatgt taaactcgtg cttcgcaatc atcgtatcta caaaacttta tacaaccagg
     6601 atcaatgagc caggcgccac tcagccgcct tgctgtgatc ggcatcaccg ccgattcctc
     6661 ggcgtgcgaa caaaccggct ttaagaccaa ccgagaagga ttgtattctc tagctagccg
     6721 ctactgcgat gccatcgaaa acgccggcgg tttgccgatc attattccgc atttgcgatc
     6781 gcgaattcag attggccggc tgttggggcg aatcgacggg ctgctgatta gcggcggagg
     6841 ttttgatatc gatccggcct attatagcga gcagcggatg gccaagctcg gcaagattaa
     6901 cccgcaaaga acgtttactg aattggaatc tattgccttt gggcttgacc gagatttgcc
     6961 gatgttgggg atctgcggcg gcgcacaagc gatcaacgtc gcccttggcg ggtcccttta
     7021 ccaagatatc gcaacacagc ttcccgccgc gcaaaggcat cagcaagagg agcgcagcga
     7081 tagccatggc cacgtagtcg aagtgtcacg cggcactcta ttgtacaaaa tttgcgggcg
     7141 cccgagacta aaagtcaaca cgacccacca tcaggcgatt cgtaacatcg gagcaggtct
     7201 cacggtcaac gcaatcgcgc ctgacggagt gatcgagggg atcgaaagca aagatcactc
     7261 gtttgttctg ggcctgcaat ggcaccccga agttctggct ccgagagagg ttatccagcg
     7321 aaagatcttt tcctccttcg tttcagcctg cagacggctg caccgcggca tttaggggcg
     7381 cctcccagcg gagttgtcga tcttgtcgac agctttagct tctgcgcgcc gcaagaagtc
     7441 gtccctgcgc caactctccc gttgaaattt tagccggtct agggcaagac aaggcgagcc
     7501 gttcaggttt aaacccggga ggtatttcat ggcgaatcag gtcggagatg ttctagataa
     7561 aggcgctgcc gaacgtgcgt tattcccggc tggcgggctg cctggcggtg gacagctgga
     7621 agtgcgcatg gagctttcag ccgatcaccc ggacccggac attcgccaag tcgctcggaa
     7681 cgtcaagcca tacaatctgc aaagctggca ttcagagtgg agcgcggcgg cggacaagaa
     7741 cgaagagctt gcggcaggat tcgttgctga agagcgcaac gttaccgcgc acgagtttta
     7801 tttgcgcgcg gcagatttct accgtcgggc gctggtatat ctgcctgaga gcgattctcg
     7861 gatgctcccg acttacgtca agcttaggga gactttccaa aaagcatgga gccttgtccc
     7921 accacccttt gaacgcgcgc aaatcccgta cgaaggtcac ctgcttgagg cgctcttttt
     7981 cccgggccgc ggcggagcgg gagctccttt gccggtggtt tacaactatg gcggcgcgga
     8041 cggcattctc ttgaggggcg aagacggcgg cgccggtcag tacgtccgcc gcggcatgca
     8101 tttcatcgat gtcgatggac cgggccacgg cggcgcgctc cgcgagaaaa aactttacgc
     8161 cacggcggat tccgaacgag tggcgaaggc ggtgattgat taccttgtct ctcgccctga
     8221 tgttgatgcc gagcgcatcg gcctgcacgg ctcgagcatg ggaggctatt ccgcaccgcg
     8281 ctgcgccact gcggaaaagc gcatcaaagc ggtcgctgtt tggagcggcg cgtacagttt
     8341 agtcgatgat attttcgatt attacccgcc gattcgagat cgtttgcgat ggctgatcgg
     8401 cgccaagaat ttggacgagg caagggagaa gataaaagaa tttaatcttg agggtcgcgc
     8461 aaaccggatc gaatgcccgc tgctggttgg ctacagccat gatgatcgtg tgatggatcc
     8521 gcgcggcgcg ctcaaacttt acgaaagcgc ggtcaattcg ccaagtcgct cgatgctaga
     8581 tggagtcggg cacggcacca agcgtttcga ccgccgcacc tacattgccg attggtttat
     8641 gaagcagctc aaagccggac aataatacgc gaccggcagc tcacctagcg gccaccgagc
     8701 gatgttcgac catcaagcag cggtcctgca cgacctgaat gccggcgcga gccaacgtct
     8761 cggcggctgg gtcgttgcgt atgccggatt gaaaccacac agctttcggc ttcttcgaca
     8821 gaatatcgtc tatatggccg ttgatatcct gcggccggcg aaagacatcg acgagatcga
     8881 tttctccggg aacgtcgacc agacggcgat agactctggc gccaaagatc tctgtgacct
     8941 cggggtagta gaccggaacc ggaacaatct cgaagccggc tccaagcaga tattggggca
     9001 cgtacacgga cggttgaccc gcctgcgctt ctgtcttaat accgagaacg gcgattcggt
     9061 gggttttcat aatcaattgt cgaatctgcg cccgggtttc gatgagattg tctttccact
     9121 ccatgccggc agtatagcaa ttgccgtctt gacaagcgac gaggttccat ccgggccaat
     9181 gaacagctat aaactgcgct gtcaatcggc gggtctcaca ttcagcttac cggcgctccc
     9241 taacctcgcg aggaccttcg taaaactata ccgcatcaga atagatgcca tcatattatt
     9301 gcgagatttc acatggccga cttgaaagta atcggcaaag ccatcactcg ggaagacggt
     9361 tacgataagg taagcggccg gacaatctat acagcggacg tgcatctagc ggggctgctc
     9421 tggggaaaaa ttttgcgcag cccttatccc cacgcgcgca tagaacgtat cgatacatat
     9481 aaagctcagc gggttccggg agtaaaggca gtcatcaccg gggccgacgt caaaggttgc
     9541 ctaatcggta aacagatccg tgatatgccc gcgctttgtt gggataaagt ccgtttcatc
     9601 ggcgaccgag tagccgccgt tgccgccgaa agcatcgacg cggccgagga agctctaact
     9661 cttatcgagg tagactacca ggaactgccc gcggtattcg atcccttgga ggcgatgcag
     9721 ccggatgcgc cgcgcctgca tgatgatatc gccgcctacg gcggcgcgcc caaagacctg
     9781 ctggctctgg atctacccaa tggcttaacc cggctggcgt ggcgcaaggg ggacgtcgag
     9841 caggcgatca atcaagcaga cgtagttctc gagcatacct ttaggatccc cgcgcggcat
     9901 caaggctatc tcgagcctca cgcggcgatc gtcagcattg ataaaaatga tcgtattcaa
     9961 gtatgggttt cagcgaaaaa cccgttcggg attcgcagtc aaatggccaa agccttggat
    10021 atttcggaag agcgcatttg cgtcaacgtg gtcaacgtcg gtggcgaatt cggcggcaaa
    10081 ggagatgcaa ccgatctgcc ggttgcctac tttctagctc aaaggtcctc ccgtccggta
    10141 aagctcgtga tgaattacgc cgaggaattg tcggccggca atccagcgca tcctaccata
    10201 ataaccatac gaagcggcgt gaaccgtgac ggccgcatcg ttggacgttc cttgagggcg
    10261 atccataccg gaggcgctta cggcgggctt aaagccaact catcgttggc cacctggcac
    10321 tatgtcggtg tcccctaccg ggttgaaaac tcccatttcg aatttttgca gatttacacc
    10381 aacactgtgc ctggcgggta ctatcgcagt cccggcgcag tggcgatggc atttgctctg
    10441 gagtcgcata ccgatctgat cgcctcagag cttggcatcg atccggccga gtttcgcctg
    10501 aaaactctcc tgagcgaagg cgaagaagac gccgtcggtc atcgattacg cgacgttcgc
    10561 tttcgtgcgg tgctggaggc agcgttggaa gcggccaaat ggcgagattc aaaacttcgc
    10621 gctaacgtcg gccgcggcat ctcgctctcc gcacggcata tcagcggcgg ggacacaggt
    10681 cttgttctaa gcgcgcaggc cgacggaagc tttatggtgc taagcccgac catcgatcaa
    10741 ggctcaggca ctcataccat attgcggcaa ctcgtagccc agcagatgaa tgtcccggtt
    10801 gaaaaagtgc gagtggtgat aggcaatacc gacacggcgc cgcgtgacag tggcgtcaga
    10861 gccagtcgag ttacctatgt ggccggtaac gcgatgatga aggcctgtgg cgaactacgc
    10921 gagcggctcc tagagcgcgc cgcgggtttc ttggagtgcc ggccggatga gattgaattc
    10981 agcgacggca gcttttgctt gcgtcaagat ccaagccagc agctgaccct gcgacggctc
    11041 gtttcccaag ccaaggatcc tctgcaggtt tcggtctatg aagactaccc gtatcccgac
    11101 gaagtgagtt acatctgcgc acagatcgcc gaggttgaag tcgaacccgc aaccggcgcg
    11161 gtgcgcgtcc atcggatgat aaccgcccac gatgtcggca cggtcattaa cccgctgacc
    11221 caccagggcc aaatcgatgg cgcaacgatt atgggtgttg gacagggcat catggaagag
    11281 ttgatcatga tcgatggcag gatcgtgaac aacaatctcg gcgactacaa gcttcccacc
    11341 attgccgaca ttccaatgct caaaaccgtc ctggtgcact ccgacggcgg tgtcggcccg
    11401 atgaatgcca agccaatcgg cgaatttgct aacaacggcc cgcccgccgc aatcgccaat
    11461 gcgattgcgg atgcagtggg tgttcgcctc ttcgagttgc cgcttaccgc ggaaaagatt
    11521 tattccttgt cgaaaggatc acagcggaaa attgctagag aaatcccgta gctgcctgtt
    11581 gctgcaagcc aattgggcgg ttctacctgt ccaatgtaag gctatcagcc taatcgacgc
    11641 cctctagctt tctctttctt tcgatctccg gcaggatcga ttcgattcgt ctcaggtcct
    11701 gcaagctcgc atcacgcagc acgtttctca cgccactggc tactcgggga aaacgatctc
    11761 tgtcaatgtc cttgattacc atggccgttg cgtcagccac agcaccgagt cgggcccgcg
    11821 agataggtgt gtctccggca ccagccgttc gatgatctgt gcgcgctctt cgctcgcttg
    11881 ggcgggtgaa atagttttat tatcaaggcg agaccggccg cccgatccac tgcaagcagt
    11941 aatcaacacc agcaaacttg ccaatacaaa tagcaggtag ctcatgttag aagtcctctt
    12001 cccgaagctg tgatttctct tcctcgaaat tacgatcaca gtccctacca cagatttctc
    12061 cagcgaaaca ctataattct cttcggggtc tgccttacag aagccgcgcg gaagctcacg
    12121 gcgaaagtgt tagcgaccct ccttgtcgtc gccaacagca aaccgatcgc atgatgattg
    12181 acaataaact caccaatcaa atactttaac gaacaaatcg atgtgctcct cgagtgattc
    12241 ccggcgaata gaagcgaaat attggcgacc gatctcgcaa acccgcggtt cccgcgttag
    12301 acacagcttt aggaggttca ccatgctcga tggaaaagta gttatcgtca ccggcgccgg
    12361 ccaggggata ggccggcatg cagcgacaac ttttgcgcag cagaatgcaa aggtcgtgat
    12421 cgctgacgtg aacgaagaaa aggcccgaaa gaccgcgacc gaacttgctc ggctcactga
    12481 agcgattgcg ctatccgtgg acgtgcgcga cgaagaaagc gttaaacgta tgatcgatca
    12541 ggtggtggat cgttttggtc agatcgacgt gcacgtcaac aacgccgcca tcgtacccca
    12601 ttttgcttgg ggtattccgc gctggccccg catcggcgag atgcccaagg atttttggga
    12661 tcgggttatt caaaccaacc tgaacggcac atttaacggc accaaacatg tcatccccca
    12721 catggagaaa agacaaaccg gtcacatcat caatctttac ggcggcggtg gaatcactcc
    12781 ggcgggagcc tgcgcctaca tggtgagcaa agacgggatt cgaaccttta cgcgctatgt
    12841 tgccgaagag gtcagaagtt ccaatatctg catcgtgacc ttttcgccgc gggtgccgat
    12901 tgtgaccgaa gatgcgccgg aagaagcatt caagcgcttg cccggccccg aaattctcgg
    12961 gcagggattt gttctcgccg cgcagctccc gatggaacaa agcggtggtt gctttgcata
    13021 cgacaatggc aagctggtga gagaaactac tagagagggc tgatcacggc agtgtgataa
    13081 aaggtcatcg tctaacagct aactatttcg agaatatgcc tgctaaatcg cgggtacagc
    13141 tgatgcctat gcaaggagta gagaatgcgg gtgattgatg cggatgctca cgtcatcgag
    13201 aatgagtcca cctgggatca tatgctcgag tccgagcgcg gctttaaacc gcgcatcgtc
    13261 ggtgcgagtg acggggacgc atccgccgat tattggttaa tcgacgggcg cctcatgccg
    13321 cgcagcaacg tcgaaaagag tttgcccgaa gccgcccgcg acatgagcga tctgagcatt
    13381 cgaatcgctc acatgaacga attgaatatc gacctccagg taatctatcc aacgattttt
    13441 attttcccaa ccgctcggcg gcccgaagtc gatctcgccc tttgccgcag ctataaccgc
    13501 tggatggctg atatcgttag taaagagccc gagcgatttc gctgggtcgt ggtcccgcct
    13561 ctgctcaata tggattctgt cgctgaggaa ttgaaattcg ctaaggagcg tggcgcgtgc
    13621 ggagtttatc taagaggctt ggaagccgac cggcgtttga gtgattctta tttttttccg
    13681 ctgtatgacg tggccagcaa actcgatctg ccgatttgcg ttcattcggc caacggcagt
    13741 atcgaggcgt acgacttttt cctcgatgaa cctggcttct gcaaattcaa gctggcggtg
    13801 gtcggcgcat ttcattcgct tctggtcgat ggcatcccgg agaagtttcc gaaattgcgg
    13861 attgccatcg tcgaagtcag cgcgcaatgg attccttatg cgatccatga tttggcgcga
    13921 aggtaccaac gccgcggcaa acagcttggc tctcgggtgt tggaggatca ccgggtttat
    13981 gtcacctgcc aaaccgacga tgacctcgac tacatcctcg gttatgccgg cgagaataat
    14041 ctcgtcatcg gcaccgatta tggccatgca gacaacgcca gcgagatcga agcactgcgc
    14101 aagttgcgaa ccgagggaaa gctcagcgcg cgggtgatcg acaagatact cggcgacaac
    14161 ccatgcgcgc tttactcgct ctaatgccgg agccgtcgca ctcaaagtcg gcggtgtcga
    14221 cgttagcgcc atgcccgcga gggggaatcg tggctctgtt ggcgtcgcgt ataacgagcc
    14281 gaagcacgat ccagtgatat agcctcgcgc ttcgaatccc aggacttctc atgagtgata
    14341 tcttcgatct cctgctcacc ggcggcaccg ttatagatcc taggtccggc accaatcaga
    14401 agttggacat cggtatcatc gctgatcgga ttgccgccat ccggtccaac atccctcctg
    14461 tgaacgccag gaaatcgctg aacgtcgagg ggtgcatcat cacgccgggc ctgatcgatt
    14521 ttcacgtgca cagttattgg ggagtaaatc cttatggctt taatgccgac ccgatttgcc
    14581 aggccagcgg cgtaacaact actatggatg ccggttcttc gggaccgact aatttcctgg
    14641 ggtttcgcaa actggtctat gagcaatccc atactcgcat gctcgccttt gtcgcaatgg
    14701 cgcagcatgg cgtgctgaac gatccggggg agttagagaa cttacgtttc gccgatccgg
    14761 aaggagcggc acaggcagtc gctgaaaatc gcgatgtcgc catcggcatc aagttgcgca
    14821 tgcacaggaa atccgtcggg gaaaacagtc gcgaggcttt acggttggca gtcaagaccg
    14881 gtgaagcctg ccgcgcgccg gtcatggtcc acgtcggcga cacagttatt tccatggacg
    14941 agattgtcga ttcgctgcgc gccggggatg tggtcacgca ttgttacacg ccgcagcagc
    15001 cttctatcat cgataaaaac ggccggcttc tcgatgcggt tcggaaagcc caggaaagag
    15061 gcgtgatctt cgatgtcggt catgccggcg ggcatttcga ctttgatctc gtgcgccgcg
    15121 ctatgggcgg cggtctgctg cccgacatca tcagctccga cttgcatggc cggttgaaac
    15181 aaccggggtt tggagttgtc ggcgatttgc ctacggtaat gaccaagttt ttggcattgg
    15241 gattgagtct ggaaaggatc atcgccgctt gcacgaacaa cccagcccga gccgtcggtt
    15301 ggcaagaccg tattggcagt ctcgaagtcg gccgggaagc ggacatcgcc gcgctggaga
    15361 ttctcgacga tcccgtcatg ttgagagatt ccgtgggcgt cgagaaactg cacaggcggc
    15421 gtatcgcggc gaggtggacg gtgcgtgccg gggagttgtt tcaggcgcga agatagaaag
    15481 tttctatata gttatccatg cggttcattg agaagagttc ggcacagcac atcggattga
    15541 agggtcaaaa tgcgaagagc aattcttttc ttgtttgtaa ttgtcgtcgc gacgagcagc
    15601 cgtgctgccg cgcagacgcc ctattatcag ggcaagacga tcacgataat caccggtagc
    15661 caggccggcg acctgttcga catttacgca cgcatgatcg caacgcatat gggaaaaaac
    15721 attcccggta atccgagtat tttggtgcag aacatgccgg gtgcgggaca tattgtcgcg
    15781 gcgaatttcg tttacggcgt cgccaaaccc gacggtttaa cgttgctcgc gcccaacccc
    15841 aatctctatg ttgaccagct cattggcaga acggaaatca aattcgattg ggccaaattt
    15901 gcttggatcg gtaatgcctc gcgcacgacc gacgtgcttt atatgcgcac cgatgcgccg
    15961 ttcaaaacta tggaagaagt gcgcagtgcc aaagaaccgc ccaagtgcgg cgcaacgggt
    16021 accgggacga ccgggtatct ggtgccgaga ctgttggacg aaaccatcag cgccaagttt
    16081 aacatcgtca cgggatacaa aggcggcaac gagatcgact tggcggtcga gcgcggcgag
    16141 ctacagtgcc gcgcgttcag cgtcacggca ttcttcggcc gcgaaccgtt tcatacctgg
    16201 agaaaaacaa actttgtgcg ggccatcatc caattgggcc aaaagagaga taaccggttg
    16261 ctggatgtac cgaccctcaa tgagttaatg gacaagtata agacacccga catcggccga
    16321 cgattagccc taatcgtaac cgcctcggaa atctttcaac gtccctacat gggtccgccc
    16381 ggcatgcgtc ccgaacatgt caggatcctc cgcgaggctt tccacaagac gttgaaagac
    16441 agtgcattca tggaagaagt gaaaagcaag aagctcgata ccgagtacac ttcgggcgaa
    16501 gagatggaaa ccttggccaa ggagatgatg tcccagcggc cggaggtcat cgaacgattg
    16561 aaaaaagtgt tgggaaacta agtcgcgact cagcaataga ttagttcaaa ccgtgaaagc
    16621 cttccaaacg tgcaacctgt ttcaagacgg acacctatcc tgggatcgcc tgcttcttca
    16681 ttggattcgt cacggattct cacgtcagaa aattccccgt ccttcgaaga gactcgggat
    16741 aaactcggtg
//
LOCUS       DASUVS010000399         3614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1047658, whole genome shotgun sequence.
ACCESSION   DASUVS010000399 DASUVS010000000
VERSION     DASUVS010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047658"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..155
                     /locus_tag="VF182_21425"
     CDS             12..155
                     /locus_tag="VF182_21425"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX6439692.1"
                     /translation="MSKGAKPADLPVELPMRFELLINLKAASQNGLTIPANVLARADK
                     VIR"
     gene            377..1186
                     /locus_tag="VF182_21430"
     CDS             377..1186
                     /locus_tag="VF182_21430"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEX6439693.1"
                     /translation="MFKLSDLIRKIFLTGIAAFVFMSHYEAVAYGWADKGHRTVAILA
                     DTRLTEHSREEVKKLLPTGTTLADAAVWPDAEGRRMTELDRLHYVNIPDNAAGYNQER
                     DCEARNCMVEALKWFTRVIADAEAPIDVRLIALRYVAHLVADMHQPLHAGRREDRGGI
                     DITVSYRAQITNLHLFWDINLVEMEEGDAGKLAKKLDESITEKERTKWQSGDPKKWTD
                     ESFRLSRSNAYNFGESMELSDEYVATARRIVRRGLAQAGVRLAWLLNNAFK"
     gene            1298..2353
                     /locus_tag="VF182_21435"
     CDS             1298..2353
                     /locus_tag="VF182_21435"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439694.1"
                     /translation="MRGRFPILCPSFRFDNPKPKNDNLKLPWLSTLAFVLMIAGVVRI
                     ADAQQAKKVPRIGYLAAVSPAADAPRKEAFRQALKDFGYVEGQNIVIEYRHEERNFER
                     LPKLAAELVELKSDVFVTVTTNAALAVRNANRSIPHVFMGVTDPVTAGLVDSLAHPGG
                     NSTGVTNMAAILSGKRLELLKEIVPRLSRVAVLWDPQAPGSAPQWEDSQVPARELNLQ
                     LHSMQVSSADKYEAAFKEAAKAQSGALAVTLNPLANSNSKRIADLAMHYRWVSICARA
                     DYADNGNLMAYGPGYSNEGFDGARYLARILKGAKPADIPVEQPTKFEMVINLKTAKQI
                     GVTIPPTLLARADRVIK"
     gene            2403..3368
                     /locus_tag="VF182_21440"
     CDS             2403..3368
                     /locus_tag="VF182_21440"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439695.1"
                     /translation="MTKELVLPTLCSLLLATYSTVQAQQVSKIPRIGIVTGISNDPSS
                     RIKTFREALQELGYFEGKNILFEYRNNEGDRSRVPGIVAELVRLKVDIIFSTQAIVIR
                     EAKRVTKTIPIVMAITPDPVKSGLVDSLARPGGNITGFTSATRELSGKRLELLTAMIP
                     GLSRVGILSGFGAFRDYEDAARSLNVPLKILEVRAPTSELPKLFQVAAKERLSAIITT
                     SVPSLSVHRKQIADLALQNRLPLMSESVTVVEDGGLASYDAHRDEIYRRAAIYVDKIL
                     KGAKPADLPIEQPTKFELVINLKTAKQIGLIIPPNVLARADKVIK"
BASE COUNT          912 a          919 c          977 g          806 t
ORIGIN      
        1 atgtcgacaa gatcagcaaa ggagccaagc ctgcagacct acccgtggaa cttcccatga
       61 gattcgaatt gctgatcaat ctgaaagctg ccagtcagaa cggcttaacc attccagcca
      121 atgttttggc tagagctgac aaagtaatcc gatgatcatt aatccaaaat ccaaaaatcg
      181 caaatccaaa atcgaatgat tccgacgaat gtgctggcgc gcgcataagg tgatcgggta
      241 aagacaaagt tatcctagcg cacctgctag atttaagctc gccgcggccg cctatgctgg
      301 aaggttttag ggcgttaccc gggatacttt gttatgcgcc gagcttgttc ggcggaactc
      361 acttgaagtg catgtcatgt ttaagctttc tgacttgatc cgaaaaatct tccttaccgg
      421 tattgctgca ttcgtgttca tgtctcatta cgaggccgtc gcctatggct gggccgacaa
      481 aggccatcga actgtagcta ttctagctga cacgcgcctc accgagcact cacgtgaaga
      541 ggtgaagaag cttcttccta ctggtacgac gctggcggat gcagctgtgt ggcccgacgc
      601 agaaggacgg cgaatgaccg aactagaccg cctgcattac gtcaacattc ccgataacgc
      661 cgctggttac aaccaagagc gggactgcga agcaagaaat tgcatggttg aagcattgaa
      721 atggtttact cgggtcatcg ccgacgctga ggcgccgatc gacgtgcggc taatcgctct
      781 acggtatgtc gcccatcttg tggctgacat gcatcagcct ctacacgccg gcaggcgaga
      841 agatagaggt ggaatcgaca tcaccgtgag ttatcgagca cagatcacta acctccatct
      901 gttctgggat atcaaccttg tcgaaatgga agagggcgat gccgggaaac tagccaaaaa
      961 attggacgag agcatcaccg agaaagaacg cactaaatgg cagtcgggag acccgaaaaa
     1021 atggaccgat gagtcgtttc gtctttcacg atccaacgcc tataactttg gagaatccat
     1081 ggagttgtca gacgaatacg tcgcaaccgc tcggagaata gtgagacgag ggctggctca
     1141 agcgggagtc aggctggcgt ggttgctcaa taacgcgttc aaatgatggt gcgatcgcgg
     1201 atcgttcctt gtgacattca ccgcgctctt gtgagagccg ataaatcatc aagcgattat
     1261 tgaatttaaa tcgtattttc gaaattagga aaaataaatg cgcggcagat ttccgatact
     1321 gtgtccgagt tttcgattcg ataatccaaa acccaaaaac gacaatctaa aattgccatg
     1381 gctcagcact ctcgccttcg tactcatgat agcgggagtt gtgaggatcg ctgacgcgca
     1441 gcaggcgaaa aaggttccgc gcatcggcta tttggcggcg gtatcgccgg ccgccgatgc
     1501 gccgcgaaaa gaggcattcc gtcaagcatt gaaggatttc gggtatgttg aggggcagaa
     1561 cattgtcatc gagtaccgcc acgaggagcg gaattttgag cgccttccga agcttgctgc
     1621 cgagcttgtc gagctaaaga gcgacgtgtt cgttacggtc actaccaatg ccgctctggc
     1681 ggtccgcaat gctaatagga gcattccgca tgttttcatg ggagtgacgg atccggttac
     1741 cgccggactc gtggatagtt tggcgcaccc cggcggcaac agcaccgggg tcaccaacat
     1801 ggcggcgatt ttgtcgggca aacgactcga gcttctgaaa gagatcgtgc ccagactctc
     1861 gcgtgttgcc gtgctgtggg atccgcaggc gcccggctct gcgccgcagt gggaagacag
     1921 ccaagtcccg gcgcgggaac tcaatctcca acttcattcc atgcaggtaa gcagtgcaga
     1981 taaatatgag gcggcgttca aggaggcagc caaggcgcag agcggcgctc ttgccgtgac
     2041 gttaaaccct ttagccaatt cgaattcaaa gaggatcgcc gatttggcga tgcattatcg
     2101 ctgggtatcg atatgcgcgc gagcggacta tgcggacaac ggcaatctca tggcttacgg
     2161 accaggctac agcaatgaag gcttcgatgg cgctcgctat ctggcgagga tcttaaaagg
     2221 cgccaagccc gccgatattc cggtcgagca gccgaccaaa tttgaaatgg tgatcaattt
     2281 aaagacggca aaacagatcg gcgtcaccat ccctccgacc ctgctggcgc gggcggacag
     2341 agtgatcaag tgatagcgat caggtgtcag ccgtcgggag atcaaaaaac ggaagccgag
     2401 tgatgacaaa agaactcgtc ctccctactc tatgctcctt gctccttgcg acctactcca
     2461 cagtccaagc gcagcaggtt tcgaaaattc cgcgcatagg aatcgtcaca ggcatttcca
     2521 acgatcctag ttctagaata aagacattcc gggaagcgct gcaagagctc ggctactttg
     2581 agggcaagaa tattttgttt gaatatcgca acaacgaggg agataggtct cgcgtcccgg
     2641 gaattgttgc cgaactcgtc cgcctcaagg tcgatatcat tttctctaca caggcgatcg
     2701 tgattcgcga agccaaacga gtgacgaaaa ccattcccat tgtcatggca attacgccgg
     2761 atccggtaaa atctggtctg gtcgatagtc tggcgcgtcc gggaggaaat attaccggat
     2821 tcacctccgc gacccgagaa ttaagtggca aacggctaga gctgctgacc gcgatgattc
     2881 cggggctctc gcgggtcgga attctttcgg gattcggcgc atttagagat tatgaggatg
     2941 ccgcacgtag tttaaacgtg ccgcttaaaa tcctcgaagt gcgagcgcca acttccgagt
     3001 tgcctaagct tttccaagtt gcggcaaaag agcgcctcag cgcaattatt acaaccagtg
     3061 ttccatcgct ctctgttcac cgaaagcaaa tcgccgatct agcgctacag aatcgactgc
     3121 cgttgatgtc ggaaagcgtg acagttgtcg aagatggcgg tctcgcgtcc tatgatgctc
     3181 atcgagacga gatttaccgg cgcgccgcga tttacgtcga taagatcttg aaaggcgcta
     3241 agccggctga tcttccaata gaacagccaa cgaagttcga gctggtgatc aatctaaaaa
     3301 ctgccaagca gatcggcctt ataatcccac ccaacgtgct ggcgagagcg gacaaggtga
     3361 tcaagtgatc ccattgcgga ctttggattt tagattttgg attggaagaa tcaaaagaca
     3421 aacaggactt tgtctgacgc ttttactttt actctttgcg ttttgcttcc cagtaagcgc
     3481 gcagcagcca aaactatcga aggtcggctg gctcggctcg cgttcagccg agagtccagg
     3541 ctccggccgt gagttgttcc ggcgcgctct cactgaactc ggctatgttg agggcaaaaa
     3601 cctagtcttt gagt
//
LOCUS       DASUVS010000400        14500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1924578, whole genome shotgun sequence.
ACCESSION   DASUVS010000400 DASUVS010000000
VERSION     DASUVS010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14500
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924578"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /gene="rseP"
                     /locus_tag="VF182_21445"
     CDS             <1..1073
                     /gene="rseP"
                     /locus_tag="VF182_21445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX6439696.1"
                     /translation="LYSIIAAVVGLGVLIIFHEFGHFLLAKLSGVGVLTFSVGFGPKL
                     WVKKKGETEYALSAFPLGGYVKMVGEDPEEEVKAVDLERSFAHKSLLKRIAIVAAGPG
                     FNLLLAVFLLMVVFLFYGVPVLSNLVGAVEPESPAAQAGIQKGDRIVAVDSQAITAWD
                     DLSSAIKQSGGQPLNLRLQRGGEQLAMTVQPRKREVKNIFGELKEDWMIGIGSQVSIE
                     KGDPGLAVSRAFVQTYEYSKLTLVGLYKMITREVSPRNLGGPILIAQMAGQQAQEGIG
                     SFLAFLAVLSINLGVLNLLPVPVLDGGHLFFFAVEAIIGRPVSLKYREKAQQVGIFLL
                     LLLMIFAFANDIFRLFEKPGGG"
     gene            1070..1807
                     /gene="tsaB"
                     /locus_tag="VF182_21450"
     CDS             1070..1807
                     /gene="tsaB"
                     /locus_tag="VF182_21450"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX6439697.1"
                     /translation="MKILGLDTSISVASVALIEDGRIVAEDFYPRQGLLDSSGGKSRH
                     TEIILPLIDSMLQGERVGLSEIAGIAVSIGPGSFTGVRIGLSTVKGLAYGADIPAVGI
                     STLQAIAARVIGFDGIVCPILDARKREVYAAVFRKQGSQLERLTEDGLMPVLSFLEEL
                     RGLPEAAPYLFIGDGVNVCGQAIQEAAGLQIYTADEDGMPSLAASVALLSERQFIDRR
                     VAQLEDLVPVYLRRPECESRIKLTVSS"
     gene            1898..2128
                     /locus_tag="VF182_21455"
     CDS             1898..2128
                     /locus_tag="VF182_21455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX6439698.1"
                     /translation="MENSEKMAIASLLDRDPELKQYYEEHQELEKKLDQFQHKLHLSP
                     DEEVEKKRLQKMKLAGKDKIMEILGRYRQTSA"
     gene            2144..3922
                     /gene="ilvB"
                     /locus_tag="VF182_21460"
     CDS             2144..3922
                     /gene="ilvB"
                     /locus_tag="VF182_21460"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX6439699.1"
                     /translation="MKMTGAEIFCESLRREGVKTVFGLPGGVVLKIFDVLCQQKDIEL
                     VLTRHEQGAAHMAEGYAKATGKAGVVLVTSGPGMTNIVTGLADAYMDSVPLVAFTGQV
                     STDLIGNDAFQEADNVGISRPCTKHNVLVKNVNDLAQTIKEAFYIATSGRPGPVLVDI
                     PKDVSTAKAEFKYPETISLRGYNPVIAGSKQHIRQAAEEIMKARKPMIYVGGGAIFSN
                     ASDEIRELAEITQIPVTMTLMGLGSFPGTHPLSLGMLGMHGGYWTNMAMHHADLLIAI
                     GARFDDRVTGKLSEFCPEARVVHIDIDPTSIKKTFHAHIPIVGDVKAVLRQMNVILRA
                     MDGNPAKIRAYRAPWLKQVQEWRTTHPLSYKQDDKVIKPQFVIDKLFELTKHEAIVAT
                     DVGQHQMFAAQYFKGTQPRTWLTSGGLGTMGFGFPAAIGAQKAFPNRMVLCITSEGSF
                     QMNLQELVTCAEQKLAVKIVLLNNGVHGMVRQWQDLFYEGRYSASLLGKMPDFVKLAD
                     AYGIKGLRALKPSEVEPVLKEGLKHKGPVLLDIHTDPYENCYPMIPAGGAQHEMMLED
                     PPELIRGKKGGAQKRKVDEGEGILPA"
     gene            3934..4455
                     /gene="ilvN"
                     /locus_tag="VF182_21465"
     CDS             3934..4455
                     /gene="ilvN"
                     /locus_tag="VF182_21465"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX6439700.1"
                     /translation="MEHIISVLVENKFGVLARVAGLFSARGYNIESLSVAATLDPTTS
                     RITIVTVGDDPIIEQIMKQLNKVVEVLEVVDLTETDFLDRETALVKVHTKPEHRDEAV
                     RIADIFRAKIVDSSLTTYTIEITGDVDKVEALINMLKPLGIKELIRTGRIGIARETTR
                     TASPRREPALLKS"
     gene            4635..5651
                     /gene="ilvC"
                     /locus_tag="VF182_21470"
     CDS             4635..5651
                     /gene="ilvC"
                     /locus_tag="VF182_21470"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX6439701.1"
                     /translation="MQVYYDRDADLKHLSGKKVAVLGYGSQGHAHANNLRDSGVDVVV
                     GLRRDSSSWSKAENAGLKVLETAEAAKVGDIIMILLPDELQGDAYEKEIKPGLKNGNY
                     LAFGHGFNIHFKRVMAPENCNVFMVAPKGPGHLVRSEFEKGRGVPCLLAVHQDPSGDT
                     TQVGLAYASALGGARAAVIQTTFKDETETDLFGEQAVLCGGLTALIQAGYETLVKAGY
                     PPEMAYFECVHEVKLIVDLIYEGGMTNMRYSISNTAEYGDLTRGKKVIGPEVSQAMKK
                     ILSDIQSGKFAEEWINEYKCGMPHFSELRKEAGNHPLEKVGANLRSMMPWLAQNRLVD
                     KSKN"
     gene            5704..6333
                     /locus_tag="VF182_21475"
     CDS             5704..6333
                     /locus_tag="VF182_21475"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HEX6439702.1"
                     /translation="MRIAKEGYPIIIFTAALSAAAWLIGWSGLALVLGVFALGVLAFF
                     RDPERCPPEGEGLIVAPADGKVVSIAKVIQGPLMKKADARVSIFLSPLDVHINRSPVG
                     GKVDDVKHTPGKFIAAYKDEASERNEQNSLKLLDDRGREVEVVQVAGVVARRIICRAR
                     KSDLLTRGARFGLIMFGSRTDLYLPASSRIEVSEGQRVKGGETIIGRFV"
     gene            6330..7187
                     /gene="pssA"
                     /locus_tag="VF182_21480"
     CDS             6330..7187
                     /gene="pssA"
                     /locus_tag="VF182_21480"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX6439703.1"
                     /translation="MNGINASEPQTNPSAKVRLLHRKTRPRVPLRQRMPAEKLKKGVF
                     LIPSLFTAGNIMCGFFSIVSTFNGEYVQAVFFIILANVLDGIDGYAARLTNTTSQFGV
                     ELDSLADVVSFGVAPAILVYFWALVPWGNWGWLAACTYVVCGALRLARYNVQAKSAAA
                     KGHFVGLPIPAAAEMIGATVLIYYYLGGEGAANKHLTLLLVIYGLALLMVSGFHYFSV
                     KSTDLRKRLPISALVFSLVLIKLAVLAPQVMFFTSFLLYTLSGPLLWCWTLLRRQRGK
                     RGGTAKALS"
     gene            7281..8843
                     /locus_tag="VF182_21485"
     CDS             7281..8843
                     /locus_tag="VF182_21485"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX6439704.1"
                     /translation="MSDSMHNHVRIFDTTLRDGEQSPGASMTVEQKVVIARQLEKLGV
                     DVIEAGFAASSDGDFESVRRVCKEVKRPRVLSLARSQEGDIERALKAVELAANPGIHI
                     FIATSDIHLKHKLRMSREQVLEAAVKAVSYAKKHIDYIEFSAEDASRSAVEFMIQVFR
                     EVIRAGASTINVPDTTGYAIPSEFGALMSNLIERTAGADRVIWSAHCHNDLGLAVANS
                     LAAVHNGARQIECTINGIGERAGNTSLEEVAMALRTRKNYLDLDTNIITEQLYPTSRL
                     VSQVTGIPIPINKPIVGENAFAHEAGIHQDGVLKHKQTYEIMTPESVGISGNRLVMGK
                     HSGRHAFDERLKHLGFNLSREDMNRAFLRFKELADKKKNVYDEDIEAIVAEEILRVPG
                     QPDCYELLYLSINSSSDSIPSATVKMRVDGHERMDQATGDGAVDACYNAITKITGSQA
                     QLVRYTVNAITGGADAQGEVSCQIQDSGIRVSGQGSHTDIIMASAAAYINALNKLQGR
                     KQYRQLVEREGP"
     gene            8840..9931
                     /gene="leuB"
                     /locus_tag="VF182_21490"
     CDS             8840..9931
                     /gene="leuB"
                     /locus_tag="VF182_21490"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX6439705.1"
                     /translation="MTYKITVLPGDGIGPEVMKQGTDVLQQLAQIYGFSVDLEEGIVG
                     GASIDAHGQPLTDRVLNLCKQSDAVLLGAMGGPKWDGLDYSIRPERALLALRQELGLF
                     ANLRPVKLFAALAAASTLKQEVVEGTDLLVVRELTGGIYFGQPKGVTKLPDGTERGIN
                     TEVYTTPEIERIAHVALQAALKRRNKVTSVDKANVLEVTELWRKVVTRVHADEGYAAI
                     KLEHMLVDNCAMQLIRNPKQFDVVVTTNMFGDILSDEAAMLTGSIGMLPSASVGGKVG
                     MYEPVHGSAPDITGQDKANPLATILSVAMMLRYSLEQSAAADRIEKAVEEVLNAGYRT
                     ADIQEKDCRPVGCKEMGRLVRDRIAATKG"
     gene            9932..10960
                     /locus_tag="VF182_21495"
     CDS             9932..10960
                     /locus_tag="VF182_21495"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX6439706.1"
                     /translation="MKVKKPKYNIAIVGATGAVGEQMREVLEERKFPVGEMRLLASER
                     SAGQFLEFDGHQQRVGVLNESSFQEIDIALFSAGGSVSAKYAPLAVSAGAVVIDNTAC
                     FRMVPDIPLVVPEVNASAIGDYKNRGIIANPNCSTIQMVVALKPIHDAARIKRVVVST
                     YQSVSGAGRQAMEELSQQIAALFNSKDVEKKKFPHQIAFNCIPHIDVFMEDGYTKEEW
                     KMIHETRKILAEPSLPVTATTVRVPVFCSHSESVNVETEKKLTAAEVRALLREAPGII
                     VADEPENNLYPLALDATGKDATYVGRIREDHSVQNGINLWVVADNLRKGAALNAVQIA
                     EILIRDHI"
     gene            11005..11736
                     /gene="truA"
                     /locus_tag="VF182_21500"
     CDS             11005..11736
                     /gene="truA"
                     /locus_tag="VF182_21500"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX6439707.1"
                     /translation="MNIKLTVEYDGTDYHGWQIQANRKTVQAVLEKAVSTLVGRPTRI
                     IGSGRTDAGVHAFGQVANFMSDKGFEPHRIQRAVNALTPDDVCVKAVEVVPDSFHARR
                     HGRSRIYQYRIWNRSSPSPFQLRYAWHVHDRLDIAAMGQAITCLDGEHDFSSFRGAGC
                     DAAHPIRTVYASSISRSDDLIVFTVEATAFLRHMVRNLVGTLVEVGRQQRSPQSFAAL
                     LDARDRTQAGMKAPAHGLFLMEVKY"
     gene            11910..12821
                     /locus_tag="VF182_21505"
     CDS             11910..12821
                     /locus_tag="VF182_21505"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6439708.1"
                     /translation="MMPAHDNFYDPYSKAKKIKIKWPNSARMAIALAGNLEAWTETPS
                     AKYRRMRHVGGSNPVKEEDVKSPYDCRTASENDYGGRTGIWRILRILDKHGVKASFNT
                     NALCIYRYPEAVKAVDARGHEIVGHSYAEDVQLIHLTEQEEREEIRTCAKMFHEFVGQ
                     KLTGWLTSGMRHTERTLGILAAEGFLWHGDAVNDDNPYPVDIDGKTLIVVPYKNAISG
                     LNDTGMYRRGITARDVFNSFKDEFDMLYEESLEDPKMLTLAMHCQMAFPATGKIYDEA
                     IQYAKSHDGVWFAKRIEIVNWIKENCL"
     gene            12950..14347
                     /gene="leuC"
                     /locus_tag="VF182_21510"
     CDS             12950..14347
                     /gene="leuC"
                     /locus_tag="VF182_21510"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012598252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX6439709.1"
                     /translation="MSGRTLYEKIWDSHTVGILPTGQTQLFIGLHLIHEITTAPAFDM
                     LREQGMDVAFPERTFATVDHIVPTDVRSRPFLDREAEELTQALERNVRQFGVEFFGLD
                     SANQGIVHVIGPQLGLTQPGMTLACGDSHTSTHGAFGTLAFGIGTSQVRDVLATQTLA
                     MEKLKVRRINIDGALGTGVYAKDVILNVIRRLGVGGGKGFAYEYGGSVIDSMHMEERM
                     TVCNMSIEGGALVGYVNPDATTFEYLKARPHVPAGAAFDRAVAYWKSIASDADARYDD
                     IVNFAAKDIEPTVTWGINPGQAVGITEKLPKPDEYSDPVGAMKAYEHMGWQPGAPILG
                     TPIDVAFIGSCTNGRLADLRAAAKIAKGRKVQSGIRALVVPGSAEVKKLAEKEGLHEI
                     FTEAGFEWREAGCSMCLAMNPDKLHGKEVSASSSNRNFIGRQGSPQGRTLLMSPAMVA
                     AAALSGKVVDVRDYQ"
BASE COUNT         3518 a         3559 c         4103 g         3320 t
ORIGIN      
        1 tgctctattc tatcatcgcg gcggtggtcg ggcttggcgt gctgatcatc tttcatgaat
       61 tcggccattt tcttcttgct aagctttcag gcgtcggggt tctgacgttt tcggtcggat
      121 ttggacctaa attgtgggta aaaaagaagg gcgaaaccga atatgccctg agcgcatttc
      181 ctttgggcgg ctatgtaaag atggtgggag aagaccccga ggaagaagtc aaggccgtgg
      241 atctcgaacg ctcctttgcc cacaaatcgc ttctcaaacg aatcgccatc gtcgccgcgg
      301 gtccgggatt taatctttta ctcgcggtgt ttctgttgat ggtggttttt cttttttatg
      361 gagtgccggt gctatccaat cttgttggag cggttgaacc ggaatcgccg gcggcacagg
      421 ctggaataca gaagggtgat cggattgttg cggttgatag ccaggcaatt acggcatggg
      481 acgatctttc cagtgcaata aagcaaagcg gaggacagcc tttaaatttg cggcttcaga
      541 gaggcggcga acaactggcg atgacggttc agcccaggaa aagagaagta aaaaacattt
      601 tcggtgaact gaaagaagac tggatgatcg gtatcggtag ccaggtttcg atcgaaaaag
      661 gcgaccccgg tctggccgtc agcagagcct ttgtgcaaac ctacgagtat tccaagctta
      721 ctttagttgg actctataaa atgattaccc gagaagtttc accgcgtaat ttgggaggcc
      781 cgatcttgat cgcccaaatg gctgggcagc aggcgcagga ggggatcgga agcttcttag
      841 ctttcctcgc ggtgttgagc ataaatcttg gagttctgaa tcttttgccg gtgccggtgc
      901 ttgacggcgg tcatcttttc ttttttgccg tcgaggcgat catcggccgg ccggtttctt
      961 tgaagtaccg cgaaaaggcg cagcaagtcg gtatattttt gctcttactt cttatgatct
     1021 ttgccttcgc caatgatatt tttcgactgt tcgaaaagcc gggtggagga tgaagattct
     1081 tggccttgat acttcgatct cggttgccag cgttgcactc attgaagacg gccgtatagt
     1141 tgcggaggat ttctatccac gtcaaggact actcgacagc tccggcggca agtctcgaca
     1201 tacagagatc atcctgccgc tcatcgattc gatgctgcag ggcgaacgag ttggactgag
     1261 tgaaatagcc ggcatcgccg tttccatcgg gccaggctcc tttacgggag ttcgaatcgg
     1321 tctaagcacg gttaagggac ttgcctatgg agccgatatc ccggcggtgg gaatttcgac
     1381 attacaagcc atcgccgcgc gtgtgatcgg tttcgatgga atcgtatgcc ctattttgga
     1441 tgcccggaag cgtgaggttt acgcagcagt attccgtaag cagggaagtc agcttgaacg
     1501 tttgacggaa gatggcctga tgccggtgct gagttttctt gaggaactac gcggtctgcc
     1561 ggaagcggcg ccttatttgt ttatcggcga tggggtcaat gtttgcggtc aggcaatcca
     1621 agaggccgct ggtttacaga tttacaccgc tgatgaagac gggatgccat ctctggcagc
     1681 ctcggttgcg ctgctaagtg agcgccaatt catcgatcgc agggtggctc agctagaaga
     1741 tttagtgccg gtttacttgc gccggccgga gtgcgaatca cggatcaagt taaccgtaag
     1801 tagttgaaat ctacgcgtta agttacattg acaagaataa gaccgtacgg tagattgagg
     1861 cgcaccaaaa acttcataag atataggagg ctcgtttatg gagaatagcg aaaagatggc
     1921 gattgcttct ctgttagatc gtgaccccga gctcaaacaa tattatgaag agcatcaaga
     1981 gctagagaaa aagctcgatc agttccaaca taaacttcat ctttctcctg acgaggaggt
     2041 tgagaagaaa cggctgcaaa aaatgaaact ggcgggtaag gataagatta tggagatcct
     2101 cggacgatac cgccaaacca gtgcctagtg taaaggaagt aagatgaaaa tgaccggggc
     2161 cgagatattc tgtgaatctt tacgacgcga aggagtcaaa accgtttttg ggttgcccgg
     2221 cggagtggtc ctgaagattt ttgacgtact gtgccagcaa aaagacattg agctggtact
     2281 tacgcggcat gaacaaggcg ccgcccatat ggcagagggt tatgccaagg caactggaaa
     2341 ggccggcgtg gtcttggtga cttctggtcc gggtatgaca aacatcgtca ctgggcttgc
     2401 cgacgcgtac atggattcgg tccccttggt tgcattcaca ggccaggttt cgacagacct
     2461 catcggcaat gacgcgtttc aagaagccga caacgtgggc atcagccgtc cctgcaccaa
     2521 gcacaatgtg ctggtcaaga atgtcaatga tctggcgcag accatcaaag aggcatttta
     2581 tattgcgacg agcggccggc cgggacctgt tttagtcgac attccaaagg atgtgagtac
     2641 ggctaaagca gaattcaaat accctgaaac gatcagttta cgtggctaca atccggtgat
     2701 cgcaggcagc aaacaacaca ttcgacaagc ggcggaagag atcatgaagg cgagaaagcc
     2761 gatgatctat gttggtggcg gcgctatttt ttccaatgcg tcggacgaaa ttcgtgagct
     2821 cgcggaaatc acccagattc ctgtgacgat gacccttatg ggcttgggta gtttccccgg
     2881 tacccatcca ctatcgcttg gcatgcttgg aatgcacggc gggtactgga ccaacatggc
     2941 gatgcatcat gccgatctgc taatcgccat cggcgcgcgt tttgacgatc gtgtgaccgg
     3001 aaagctctcc gaattttgtc ccgaagcgcg cgtcgtacac atcgatatcg acccaacttc
     3061 gatcaaaaaa acttttcacg cccacatccc gatcgtcggc gatgtcaaag cggttctgcg
     3121 gcagatgaac gtcatcctgc gagcaatgga cggtaatccc gccaagatta gagcttatcg
     3181 ggcaccttgg ttgaagcagg tccaagaatg gcgaactaca cacccgttgt cctacaagca
     3241 ggatgacaag gtcatcaagc cacaatttgt catcgacaag ctcttcgaat tgaccaaaca
     3301 tgaagcgatc gttgcgactg acgtcggcca gcaccagatg tttgccgctc aatacttcaa
     3361 gggcacccaa ccgagaacat ggctcacttc aggcggtttg ggcaccatgg gttttggttt
     3421 tccagcggct ataggcgcac aaaaggcgtt cccgaatcgc atggtgcttt gcattaccag
     3481 cgaaggcagc ttccagatga atctgcagga actggtcaca tgcgcggaac aaaaattagc
     3541 ggtcaaaatc gtcctgctca ataatggggt gcacgggatg gttcggcaat ggcaggatct
     3601 tttctatgaa ggacgttact cggccagtct tttgggaaag atgccagact tcgtgaaact
     3661 ggcggacgct tatgggatca agggcttgcg cgcgctaaag ccaagcgagg tcgaacccgt
     3721 actaaaagaa ggcctgaagc ataaggggcc tgtgctgctg gatatccata ccgatcccta
     3781 cgagaactgt tacccaatga ttcctgcggg cggcgcccaa cacgaaatga tgcttgaaga
     3841 ccctccggag ctgattcgag gcaagaaagg tggagcacag aagagaaagg tggatgaggg
     3901 cgagggaatt cttcctgcct gatttttcag cgaatggaac atattatatc ggttctcgtc
     3961 gaaaacaaat ttggcgtttt ggcaagagta gcaggattgt tcagcgcgcg tggatacaat
     4021 attgaaagct tgtccgtcgc tgccacgttg gatccgacca cctcccgcat aacaattgtc
     4081 accgtcggtg acgatccgat catcgagcag atcatgaagc agttaaataa ggtcgtcgaa
     4141 gttctcgaag tggtggatct taccgaaact gattttcttg atcgcgagac agctctggtc
     4201 aaagttcaca ctaaaccgga acatcgagat gaagcagtac ggatcgccga catctttcgc
     4261 gccaaaattg tcgattcctc gttaacgacg tacacaatcg aaattaccgg tgacgtcgac
     4321 aaggtggaag cgctgatcaa tatgctaaag ccgttaggaa tcaaggagct tattcgcacc
     4381 ggtcgaatcg gcattgctcg agagaccaca cgaaccgcct cacctcgtcg cgagccggcg
     4441 ttgctcaaat cgtaacactg ctgccggatt catcttcaga aatgccggat gggtggccgc
     4501 tagaaacttc cccgcgggac ccgataaaag cgcttttcgt catcattttc gttcgcgccg
     4561 cttacatccg gccgagtttg acatgatcct atcttatcgc catattggaa tttcgctcta
     4621 gaaagggaca attaatgcaa gtttattatg atcgcgatgc cgatctgaag cacttgtcgg
     4681 gaaaaaaggt ggccgttttg ggctacggca gccaaggcca tgctcacgcc aacaacttgc
     4741 gggactccgg cgtcgacgtt gttgtcgggt tgagacgcga cagtagttct tggagcaaag
     4801 ccgagaatgc cggtctaaag gttctagaaa cggcagaggc cgctaaagtc ggcgacatca
     4861 tcatgatcct gcttcctgac gagctgcaag gggatgccta cgaaaaagag atcaagccag
     4921 gtctaaagaa cggaaattat ctcgccttcg gccatggatt caacatccac ttcaaacgcg
     4981 tgatggcgcc tgaaaactgt aatgtcttca tggttgcacc caaggggccg ggccatctag
     5041 tgcgtagtga atttgagaag ggccgtggag taccgtgctt gctcgcggtt catcaggatc
     5101 cgtccggcga cactacccaa gtggggctcg cctacgcaag cgcccttgga ggcgcgcgtg
     5161 cagcggttat tcagacgacg tttaaagacg aaacggaaac ggaccttttt ggtgagcaag
     5221 cggtgctctg tggcggcttg acagcgctaa ttcaggcagg ctacgagacg cttgtgaagg
     5281 ccgggtatcc tcccgagatg gcttatttcg aatgtgtcca cgaagttaag ctgatcgtcg
     5341 atttaattta cgaaggcggc atgaccaaca tgcgctactc aatcagcaat acggctgagt
     5401 atggcgacct gacccgtgga aaaaaagtga tcggccccga ggtctcccag gcaatgaaaa
     5461 agattctcag tgacattcaa tcaggcaagt tcgccgagga gtggatcaac gagtataaat
     5521 gcggcatgcc gcatttttcc gagctgcgca aagaagccgg aaatcatccg ctcgagaaag
     5581 tgggcgcaaa tctgagatcg atgatgccgt ggttggccca gaatcgatta gtcgacaaga
     5641 gcaagaactg agcggtccta ttgccggaat cgatagggag tcgccgtctg tcggacgatc
     5701 cgcatgagaa ttgccaaaga aggttatcca attatcatct tcaccgcggc tctaagtgcc
     5761 gcagcgtggc tgatcgggtg gtcggggctt gcgcttgttt tgggtgtttt cgccttgggt
     5821 gtcctggctt ttttccggga tccggaacgc tgcccgcccg aaggtgaagg gctgattgtt
     5881 gcgccggcag atgggaaagt cgtcagtatt gccaaagtta tccagggtcc gctaatgaaa
     5941 aaagccgacg cgcgtgttag tatcttcttg tcgcctttgg atgttcatat caatcgttcg
     6001 cccgttggcg gcaaagttga cgacgtaaaa catacaccgg gcaaatttat cgccgcttat
     6061 aaagatgaag cttcagaaag aaacgagcag aatagcttaa agttactgga tgatcgaggt
     6121 cgagaagtcg aggttgtgca ggtcgccgga gtggtggcgc ggcgcattat ctgccgcgct
     6181 cgaaaaagtg atctcctgac gaggggcgcc agattcggtt tgattatgtt cggatctcga
     6241 accgatcttt acttgccggc gagcagccga attgaagtgt cggaagggca gagggtgaag
     6301 gggggagaaa caatcatagg acggttcgta tgaatggaat taatgcttca gaaccacaaa
     6361 cgaatccttc ggcaaaggta cgcttgctgc atcgaaaaac ccgcccgcgt gttccgctgc
     6421 gccagaggat gccggcggag aaacttaaaa aaggggtgtt cttaattccc agcttgttca
     6481 cagctggcaa catcatgtgc gggtttttct ccatcgtttc cacctttaac ggagaatacg
     6541 ttcaggcggt ttttttcatt attctggcca atgtactcga tggtatcgac ggctatgcgg
     6601 cgcgcctgac caacaccacc agccaatttg gcgtagaact cgattctctc gcggatgtcg
     6661 tctcatttgg tgtagcgcca gccatcctgg tttatttctg ggcgctggtt ccttggggta
     6721 actggggctg gttggcagct tgcacttacg tcgtttgcgg tgcattgcgc ttggcgcgtt
     6781 acaacgtcca agccaaaagc gcagccgcca aagggcattt tgtcggattg ccgatcccag
     6841 ccgcggctga aatgattgga gcgacagttc tcatttatta ctacctggga ggagaaggcg
     6901 cggccaacaa gcatctgacg cttctactgg ttatatacgg tctggctctc ttgatggtta
     6961 gcgggtttca ttatttcagt gtgaagagca ccgatttgcg caagcgtttg ccgatatcgg
     7021 ccctagtttt tagtttggtt ttgattaaac tcgccgttct ggcgccgcaa gttatgtttt
     7081 ttaccagctt cctgttgtat acattatcgg gtcccctctt atggtgttgg acgctgttac
     7141 ggcgtcaacg gggaaaaaga ggagggacag ctaaagcatt atcttaactg ccctctcttc
     7201 tgacacgcga ataactcccg ttggcactgc cgcgggagtt tttgtttctg gacagtgtga
     7261 gccctaaact tggaggagtc atgtcagata gtatgcataa tcacgtcaga attttcgaca
     7321 ccacattgcg cgatggcgag cagtcgcccg gcgccagcat gaccgttgaa caaaaagtcg
     7381 tgatcgcgcg ccaattggaa aagctcggcg tcgatgtgat cgaggcgggg tttgccgcct
     7441 catcggacgg tgatttcgaa tcggtcaggc gggtctgtaa ggaagtgaaa cgaccgcgcg
     7501 tgctcagtct ggcgcgttct caagaaggag acatcgagcg cgcgctcaaa gctgtcgagc
     7561 ttgcggcaaa tccgggcatt catatattta ttgcgacttc ggacattcat ctgaagcata
     7621 agctgcgaat gtcgcgagag caggttttgg aagccgctgt caaggcagtt agctatgcga
     7681 agaaacatat cgattacata gaattctcgg ccgaagatgc atcgcgcagc gcagtggagt
     7741 tcatgatcca ggtattcaga gaagtaattc gggcgggagc cagtaccata aacgttcccg
     7801 acaccaccgg atatgcgatt ccttctgagt tcggcgctct catgagcaac ttgatcgaac
     7861 ggaccgcggg cgcagaccgg gtgatttgga gcgcccactg ccataatgac ctcgggcttg
     7921 cggtggcgaa ttcgttggcg gccgtgcaca atggcgccag acagatcgaa tgcacaatca
     7981 acggcatcgg cgagcgcgcc ggcaatacct ctttggaaga agtcgcgatg gcgctgcgca
     8041 cgagaaagaa ttatctcgat ctcgatacga atatcatcac cgagcagctt tatccgacat
     8101 cacggctggt ctcacaggtc actggaattc caattccgat caataaaccg attgtcggtg
     8161 aaaacgcctt tgcacacgag gcgggtatcc atcaggatgg agtgctcaaa cacaagcaga
     8221 cctacgaaat tatgaccccc gagtccgtag gcatcagcgg taatcgcctg gtcatgggta
     8281 aacattccgg ccggcatgct ttcgatgagc ggttaaagca tctcggtttc aatctcagca
     8341 gggaagatat gaaccgcgcg ttccttcgtt tcaaagaact ggcggacaag aaaaaaaacg
     8401 tctatgacga agacatcgag gccattgttg cggaagagat tctcagggtt cccgggcaac
     8461 cggactgtta tgagctgttg tatctgagca tcaattccag ctccgacagc attccgtctg
     8521 ccacggtgaa aatgcgggtg gacgggcacg agcgaatgga tcaggccacc ggcgatggcg
     8581 ccgtcgatgc ttgctataat gctatcacca aaataaccgg ctcccaggcg caactagttc
     8641 gatatacggt aaacgctatc accggcggcg ccgatgcgca gggcgaggtc tcctgtcaaa
     8701 ttcaagacag tggcattcga gtatccggac agggctcgca taccgatatc attatggcga
     8761 gcgcagcggc ttacattaat gcgctgaaca aactgcaggg acgaaaacaa tatcggcaac
     8821 tggttgagag agagggccca tgacttacaa gatcacagtt ttgcccggcg atggcatcgg
     8881 ccctgaggta atgaaacagg gcaccgacgt acttcaacag ctggcgcaga tttatggatt
     8941 cagcgttgat ttggaagaag ggatcgtcgg cggcgcgtcg atcgatgctc acggccaacc
     9001 tctgacggat cgtgtcctaa atttatgtaa gcaaagcgat gccgtgcttc tcggcgccat
     9061 gggcggaccc aagtgggacg gactcgatta ttccattcgc ccggagcgcg cgctgcttgc
     9121 cctgcgccag gaacttggat tgttcgccaa tcttcgcccg gtgaaattat tcgctgcttt
     9181 ggctgcggca tctacgttga agcaggaagt agtcgaagga actgacctgc tcgtcgtccg
     9241 cgaattgacc ggagggattt atttcgggca gccgaaaggt gtgacgaaac tacccgacgg
     9301 taccgagcgc ggaatcaata ccgaggttta taccactcct gaaatcgaac gcatcgctca
     9361 cgtcgcgctt caggccgcct tgaagcggcg taacaaagtt acttcggtcg acaaggccaa
     9421 cgtgttagag gtgaccgaat tatggcggaa agtggttacc cgggtgcatg cggacgaggg
     9481 ttatgcggcc attaaattgg aacatatgct ggttgataac tgcgccatgc aactgatccg
     9541 caatcccaag caattcgacg tggtcgtgac tacgaacatg ttcggagaca tattgagtga
     9601 tgaagccgcc atgctaacgg gctccattgg catgctgcct tcagccagcg tcggcggaaa
     9661 agtcggcatg tacgagccgg ttcatggcag cgccccggat attaccgggc aagacaaggc
     9721 gaatcctctg gcgactattc tgagtgtggc aatgatgttg cgttattctc tcgagcagag
     9781 cgcggcagcg gatcgcatcg aaaaagccgt ggaggaagtg ttgaatgcag gataccgaac
     9841 tgcggatatc caagaaaaag attgccggcc ggtggggtgc aaagaaatgg gccggttggt
     9901 tcgtgatagg atcgctgcga caaagggcta ggtgaaggtg aaaaagccaa agtacaatat
     9961 cgcaatcgtc ggcgcgaccg gagcggtcgg tgaacagatg cgtgaggtcc tggaagaacg
    10021 gaaatttccg gtgggagaga tgcgcttgct cgcctctgaa cggtccgcgg gacagtttct
    10081 tgagtttgac gggcaccagc agcgcgtggg tgttttgaac gaaagttcat ttcaagagat
    10141 cgacatcgcg ctattttccg ccggcgggag tgtcagcgcg aaatacgcac cgctggccgt
    10201 ttctgccggc gctgtcgtca ttgacaatac cgcctgcttt cgcatggtgc cggatatccc
    10261 gctggtagtc ccggaggtca acgcgagcgc cattggcgat tacaaaaacc gcgggattat
    10321 cgccaatcct aactgctcca ctatccagat ggtggttgct cttaaaccga ttcacgacgc
    10381 cgcgcgcatc aagcgcgtgg tggtttcgac ttatcaatcg gtatcgggcg ccggcaggca
    10441 agcgatggag gaattgagcc agcagattgc ggctcttttt aacagcaagg atgtcgagaa
    10501 gaagaaattt ccgcatcaaa tcgcatttaa ttgcattcct catatcgatg tcttcatgga
    10561 ggacggatac accaaagaag aatggaagat gattcacgag acacggaaga tcctggcgga
    10621 gccgtccctt ccggtgaccg ccactacggt gcgcgtgccg gtcttttgca gccactcgga
    10681 gtcggtgaac gtggagactg agaaaaaact cacggctgcc gaagtcagag cactgctccg
    10741 tgaagcgccc ggcattatcg tcgccgatga gccggagaac aatctatatc cactggcact
    10801 cgacgctacc ggcaaagatg ccacctatgt gggtcgaata cgcgaagatc actccgttca
    10861 gaacggcatc aatctgtggg tagtcgccga caacctgcgc aaaggcgcag cgctgaatgc
    10921 ggttcagatc gctgaaatac tgattcgcga ccatatctga tcatcggtaa tgatccgcca
    10981 gttactcttt ccttcgttct cttagtgaac attaagctta ccgtcgagta cgacggcact
    11041 gattatcacg gctggcaaat tcaggccaac cgaaagacag ttcaagcggt tttagaaaaa
    11101 gccgtttcta ccttggtggg gcggccgaca cgtataatcg gttcgggtcg aaccgatgcg
    11161 ggagtgcatg cttttggcca ggtggccaac ttcatgtccg acaagggatt cgaaccacat
    11221 cgcattcagc gcgctgtcaa tgcgttgacg cccgatgacg tttgcgtaaa agcggttgaa
    11281 gtcgtgccgg attccttcca tgcgcggcgc catgggcgca gccgaatcta tcaataccgg
    11341 atatggaatc ggtcgagtcc ttcaccgttc cagctgagat acgcttggca cgtgcacgac
    11401 cggctagaca tcgctgcgat gggccaagcg atcacttgcc tggacggaga gcatgacttt
    11461 tcctctttcc gcggcgccgg ctgcgatgcc gcccatccga taaggactgt gtacgccagt
    11521 tccataagcc gaagcgacga cttgattgtt tttaccgtcg aagccacagc ttttcttcgt
    11581 cacatggtgc gtaatctggt tggcacattg gtggaagtcg gccgacaaca gcgctcaccg
    11641 caatcctttg ctgcgcttct cgatgcccgg gaccgtacgc aggccgggat gaaagctccg
    11701 gcacacggtt tgtttctcat ggaagtgaaa tactaaagtg cgcgccagcg agttgtgtcg
    11761 tgaggaggcg atagagtatc gcccaccggc gtgctgcgaa ttcagtccat tgagatggta
    11821 actttcgttg acactattcc tagcttccat ataccttaca gcccgaggtg aaaggatcct
    11881 gcgatgataa ccaaggaggg tgacaaatca tgatgccggc acacgataac ttctatgacc
    11941 cttattcaaa ggccaagaag attaaaatca aatggccaaa cagcgcgcgg atggcgattg
    12001 cgttggccgg aaatttggaa gcctggaccg aaacccccag cgcaaaatac cggcgcatgc
    12061 gccatgttgg cgggtcgaat cctgtcaaag aagaagatgt caaatcgccc tatgattgtc
    12121 gtaccgccag tgaaaatgat tacggcggcc gaaccggaat ctggcgtatc ctgcgcatcc
    12181 tggataagca cggcgtgaaa gcctctttca acacaaatgc gctttgcatc taccgatacc
    12241 ccgaggctgt taaagccgtc gacgcccgcg gacatgaaat cgttggtcac tcgtacgccg
    12301 aggatgttca attgatccat cttacagagc aagaggagcg cgaggagata cggacctgtg
    12361 ccaagatgtt tcacgaattc gttggccaaa aactcacggg ttggctgact tcgggcatgc
    12421 gccataccga gcgaacccta ggcattcttg ccgccgaagg ttttctctgg catggggatg
    12481 ccgtcaacga cgataatcct tatccggtcg atatcgatgg caaaacattg atcgtggtgc
    12541 cgtataagaa cgccatcagc ggacttaacg acacgggcat gtatcggcga ggcatcactg
    12601 cccgtgatgt ttttaattca ttcaaggatg aattcgacat gctttacgaa gaatcgctgg
    12661 aggatcccaa aatgctgaca ttggcgatgc attgtcagat ggcgtttccc gcgaccggga
    12721 aaatctacga tgaggcgatt cagtacgcca agtctcacga tggcgtttgg tttgccaaac
    12781 gtattgagat cgtgaattgg attaaggaaa attgccttta gattagctga tcggttctga
    12841 caatccgtgc gtccgtagat caccaaagta ctaatccaaa agcgcggaac tagatccact
    12901 tgaactcggg catagcggca gataatatcc tgcgaaggag attttgcgaa tgagcggtag
    12961 aacgctatac gagaagatat gggattccca tacggttggg attcttccaa ccggccaaac
    13021 ccaacttttc atcgggcttc atctgattca cgagattacc acggcacctg ccttcgatat
    13081 gttgcgcgag cagggtatgg atgtagcgtt tccggagcga accttcgcta cagtcgacca
    13141 tattgttccg accgatgttc gtagccgacc ctttctcgat cgcgaagccg aagaactgac
    13201 ccaagcgctt gaaaggaatg tcaggcaatt cggcgtggag tttttcggct tggatagcgc
    13261 caatcaagga atcgtccatg tcatcggtcc gcagcttggc ttgactcaac cgggaatgac
    13321 gctggcgtgc ggtgatagcc acacgagcac ccacggagca tttgggactc tggcatttgg
    13381 aatcggtacc agccaggttc gtgatgttct ggcgacacag accttggcga tggaaaagct
    13441 caaggtgcgc cgcatcaata tcgatggcgc gctcggcacg ggcgtttatg ccaaggatgt
    13501 gattctgaat gtcatccggc ggctcggcgt cggcggcggt aaaggtttcg cttatgaata
    13561 tggcggatct gtcatcgact cgatgcacat ggaagaacgt atgaccgttt gcaacatgag
    13621 catcgaaggc ggtgcgttgg tgggttacgt caatcccgac gcaaccacgt ttgagtacct
    13681 taaagccaga cctcatgtgc cggccggcgc tgcattcgat cgcgctgtcg cctattggaa
    13741 gtcgattgct tccgatgccg acgcgaggta cgacgatatc gtcaatttcg cagcaaagga
    13801 tatcgaaccg acagtcactt ggggcatcaa tccgggacaa gcggtaggga taaccgaaaa
    13861 actcccgaag cccgatgaat actccgatcc tgttggggca atgaaagcgt acgagcatat
    13921 gggatggcaa ccgggcgcgc caattttggg cacaccgatt gatgtggcgt ttatcggctc
    13981 gtgcactaat ggccggctcg cggatctgcg ggcggcggct aaaatcgcca aggggagaaa
    14041 ggttcagtcc ggaattcgcg cgctggtggt ccccggttcg gccgaggtca agaagcttgc
    14101 cgagaaggaa ggtctgcacg agatatttac cgaagcaggg tttgaatggc gcgaggccgg
    14161 atgttccatg tgcttagcga tgaatcccga taagcttcat ggcaaagaag tctcggcatc
    14221 ctcatccaat cgtaatttta ttggccgtca aggcagcccg cagggaagaa ctctattgat
    14281 gagcccagcc atggtcgcag ctgccgcact gagcgggaag gtcgtcgacg tgcgggatta
    14341 ccagtaggag ggggggacca tgagcgcaat cgtgaaaatt accaacatag aaggcagagc
    14401 ggtgacgatc cgcggcaacg atatcgatac cgatcgaatc attcccgcgc gttatcttaa
    14461 agaagtgagc tttgcccgca tgggtgagta tcctttcttc
//
LOCUS       DASUVS010000401         7460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1925432, whole genome shotgun sequence.
ACCESSION   DASUVS010000401 DASUVS010000000
VERSION     DASUVS010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925432"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1250)
                     /locus_tag="VF182_21515"
     CDS             complement(156..1250)
                     /locus_tag="VF182_21515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6439710.1"
                     /translation="MCSRLSMNRASQFEVIVASLKRRHTGVTSTVAALIPEQAKRLDI
                     AALGPKVPPQWPRIRWSDLFRHGFNPPSGKKFRIWHARRNLEALTGLILRSVLRMPLK
                     LVLTSAAQRRHSRWTHWLMRQMDVVVATSAETANYIPVPAKIIFHGVDTARYFPAEDR
                     SREWGESGLPGRFGIAIFGRVRHQKGTDLFVDAMCRLLPKFPDFTAVIIGAVTPDQIP
                     FANNLKRIIAAAGLSERIRFLGERPATEIPLWFRRMSIVVSPQRWEGFGLVPLEAMAS
                     GCAVVATRVGAAHHVILDEHTGYLVSPEDLDALVARLEILMRDPQLTVEMGQAGHVHA
                     VNHFSIEREAAELERVYESTWRGSGTRRDG"
     gene            complement(1305..2300)
                     /locus_tag="VF182_21520"
     CDS             complement(1305..2300)
                     /locus_tag="VF182_21520"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439711.1"
                     /translation="MKLRITAMVVVLLLLSFSSSPVMAQKIRAASGGLSVIHALLWVA
                     YDQKLLNKHGMDMEYIAIENGTVGMQTLVANESQFLFSTGSLAVNANLQGADITIVAG
                     GLNFIPDKLIVRPEIKSAEDFKGKRIAISRFGSSSEVNLRIALEKIGVKADQVQIIQL
                     GGVSPRQAALLSGQVQATVLSDPQATSAIKSGMNLLVDLSDKKWGLPRYCHNCFMTTR
                     KFLETHREQAVGFIKTMIEGLYMMKRDKNLAVRLIKKYLRLDDESASIGYDFYIAQHG
                     EGIMSLPEPRGLDFVIAEVAKQNSKAAGATPDSLRLLDPTIVDEIRKSGFIERVK"
     gene            complement(2356..4578)
                     /locus_tag="VF182_21525"
     CDS             complement(2356..4578)
                     /locus_tag="VF182_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HEX6439712.1"
                     /translation="MSKVTSNDTGLARWVPFGLVGQTQPRHYREMLGVIWENKTEIPY
                     AWDILKRGVCDGCSLGPYGLKDNVHEGVHLCMTRLKLLKVNTMSALDPSRMSDVGALR
                     RLAPEKLRSLGRIPYPMLRRRGEKGFNRISWDQAVELTTRSIRNAPPNQMAFFATSRG
                     LTNEAYYVFQKLARVLGTNNVDLCSRLCHAATVSGLKATLGVGAPTGSLADFIGTEML
                     VIFGSDLANNHPVTTKYMHLAKQQGTRIIVVNPMREYGLERYRVPALASSAIFGTKLM
                     DDFYQVRVGGDIAFINGILKALLAADQVHHGFIAAHTAGFEQMRAAVEQQSWELLEQL
                     SGVTRNQMIDFAQRYGTAQSAVFVYSTGLTQHEFGVDNVKALVNLALARGMFGREKCG
                     IMPMRGPSGAQGGADCGVEPDKFPGGLSVDESSARRFSNLWKHPIPARPGLRVPHMIE
                     AAGRGDLQFLYSIGGNLLEALPDRNYVARALERVPVRIHQDILLNTSMLLNAMESILI
                     LPSQTRYEQRGGGTSTSTERRIRFSPEIPGHSIGETLPEWEIPMRIGRKAMPNGELLF
                     PFDDTQSIREEMARVMPIYHGIEKLTKEGDHFQWGGPFLFKHGFTGMPENRAQFTPMK
                     PPDRRASAGKFYLSTRRGKQFNSMTFGHTDGSMGARSRHVLFMSPDDAEKLELTDGYE
                     VIIRSEIGRMKGIIQLAPLKPGTLQAYWPEANVLIPRRIDPVSGGPDYNVEVWIEKDR
                     "
     gene            complement(4597..5046)
                     /locus_tag="VF182_21530"
     CDS             complement(4597..5046)
                     /locus_tag="VF182_21530"
                     /inference="COORDINATES: protein motif:HMM:NF021192.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase associated protein"
                     /protein_id="HEX6439713.1"
                     /translation="MFYRFRYEKDLFPNLDRLPLHVRMKLDLTGIKLSLKQWLAFHIE
                     ERIAICHLPVDCEDEKQVFKDYLRFLSRRYCGAPAQELPPMEPLWDISDQVPAPVLER
                     SGDDGDAVTLEEWIGWASHQRYALYKTAVSKSEPEKFFAVLDELRHP"
     gene            5438..5962
                     /locus_tag="VF182_21535"
     CDS             5438..5962
                     /locus_tag="VF182_21535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YrzE family protein"
                     /protein_id="HEX6439714.1"
                     /translation="MQSRPPEANNPPPPIEPTGFFTGIKIRPVIIGVVVDIVATMMLS
                     TLYYFIFVAKQLSDSGAMSEEAMAQYWSSSEGIMASVVIGTLCTGIGGFYAGRKAGAL
                     EMKHGGLVGLGSILTGIILQSAGEDGTGLNQWQMLIAYASAIPAGALGGSVAEWARGF
                     APMKPPDGGTRRRR"
     gene            5970..6521
                     /locus_tag="VF182_21540"
     CDS             5970..6521
                     /locus_tag="VF182_21540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439715.1"
                     /translation="MGSIEYRRAGPDDYAEIVRLQRVNYIGNLSEEERREGFLSAEFS
                     LEQVAAIATDLGIAIVTMDDDLAGCLCGIRREFDHRSPVVAKMLDSYDQVWFEDKPLS
                     AFNSYIYGPVCLARQYRRRGLLRGLYDFQKKDLAGRFELGVALVSHSNPHSMQAHVEG
                     LGMTKTGEFELNGSLFAILAFRI"
     gene            6754..7257
                     /locus_tag="VF182_21545"
     CDS             6754..7257
                     /locus_tag="VF182_21545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX6439716.1"
                     /translation="MDKDTKKTALRMIPYGLYVLTGEGKDGKIAAATVNWVTQTAFEP
                     PLVVVGVKADSGAHAIIKESGTFALNILGKDQKGQAYTFFKALEREGDTIGGEPFRKG
                     SLGAPILQNAPAFVECQLVETIEKGDHSIFVGEVKDAGVAAQPEGRPDDATLTLKDLG
                     DKVFYGG"
BASE COUNT         1676 a         2038 c         2079 g         1667 t
ORIGIN      
        1 caggggaaat cacaggaacc atcggtttga gtaacctgtc tgccaccggc aaaggtcgcc
       61 taaccgccgg cttcgagtta accagataac gccgcgcaac gctgtcatta tgtaagaaga
      121 tggacttact tctttaatta atcgagaccc tgaaactaac catcccgccg ggtgcccgag
      181 ccgcgccaag tgctttcgta aacgcgttcc agctcggccg cctcgcgttc gatcgaaaag
      241 tgattgactg catgaacgtg tccggcttgc cccatctcga ccgtgagctg agggtcgcgc
      301 atcagaattt ctaggcgagc gaccaaggca tcgaggtcct caggagacac gagatagccg
      361 gtatgctcgt cgaggataac gtggtgagcg gcgccgaccc gagtcgccac caccgcacag
      421 ccgcttgcca tcgcttccaa cggcaccagc ccgaaaccct cccagcgctg cggtgagacg
      481 acgatgctca tgcgcctaaa ccagagaggg atctcagtcg cgggtctttc accgagaaac
      541 cggatgcgct ctgaaagacc ggcagccgcg attatacgct ttaaattgtt tgcaaatgga
      601 atttggtccg gtgtcacggc gcctatgatc acggcggtga agtcgggaaa ttttggtagc
      661 aagcggcaca tcgcatcaac gaacaggtcg gttccttttt gatgacgcac ccgtccgaaa
      721 atagcgatac cgaaacggcc gggaagtccg ctctcgcccc attctcggct gcggtcttcg
      781 gcaggaaagt agcgcgccgt gtcgacaccg tggaagatga tcttggcagg gaccggaata
      841 taattcgccg tctccgctga ggtcgcgaca acaacgtcca tttgccgcat cagccaatgc
      901 gtccaacgac tatgacggcg ctgcgccgca gatgttagca caagtttgag cggcattctc
      961 agcaccgaac ggagaatcag gcctgtgagc gcctcaagat tgcggcgcgc gtgccaaatg
     1021 cggaattttt tccccgatgg cggattaaat ccgtgcctga agagatcgct ccagcggatg
     1081 cgcggccact gcggcgggac cttgggcccc agagcggcga tatccaatcg tttggcctgt
     1141 tctggtatca aagccgcgac ggtcgaggtt accccggtgt gccgccgctt taagctcgcg
     1201 acgatgactt cgaattggga ggcgcgattc atcgataaac gtgagcacat aaggagaact
     1261 cagaagaaag atcgcttgtt cgatcagtcc actagattga agcttcattt cacccgttcg
     1321 ataaatccgc tctttctaat ctcatcgaca atggtcggat cgagcagccg caggctgtcc
     1381 ggcgtcgctc ccgcagcttt agaattttgt ttggcaacct cggcgatgac aaagtcgaga
     1441 cctcttggtt ccggtaagct cattattccc tcgccgtgct gggcgatgta gaaatcatac
     1501 cctatggatg cgctttcatc gtctaggcgc agatattttt tgatcagtcg aactgcgaga
     1561 tttttatccc gcttcatcat gtagaggcct tcgatcattg ttttgataaa cccgaccgct
     1621 tgctctcgat gggtttcgag aaacttcctg gttgtcatga aacaattatg gcagtagcgg
     1681 ggcagtcccc attttttgtc agacaaatcg accagaagat tcatgcctga tttaatcgcg
     1741 gaggtcgcct gcggatcgct cagcaccgtc gcttggactt gcccggagag caaagcggct
     1801 tgacgcgggg atacgccgcc caactgaata atctgaacct ggtcggcttt tacgccgatc
     1861 ttttccaagg cgattctgag attgacctcg gacgaggagc cgaaccggct gatggcgatt
     1921 cgtttgcctt tgaaatcctc ggcgcttttg atttcaggtc gaacgatcag tttatcagga
     1981 atgaagttga gtccgccggc aacgatcgta atgtcggccc cctgtaaatt ggcattgact
     2041 gccaatgatc cggtggaaaa gaggaattgc gactcgttgg ccaccagagt ctgcatgccg
     2101 accgtgccgt tttcgatggc gatgtactcc atgtccatcc cgtgtttgtt caacagtttc
     2161 tgatcgtagg ctacccacag cagcgcatgg ataacagata gcccgccgct ggccgcgcga
     2221 atcttttgcg ccattactgg tgaagagcta aacgacagga gcaagaggac gacaaccatt
     2281 gcagttattc ggagcttcac atttttctcc atcccatccg ataacgcgca gcgattgcaa
     2341 ctgcagtctc ccatactacc gatccttctc gatccaaacc tcgacattgt aatccggccc
     2401 gccggagaca ggatcgatcc ggcgcgggat caagacgttg gcttcgggcc aataagcttg
     2461 taatgtccca ggcttaagcg gcgcaagctg aatgattcct ttcatcctgc caatctccga
     2521 gcgaatgatc acttcgtagc cgtccgtcag ctcgagcttc tcggcatcgt caggcgacat
     2581 aaagagaacg tggcgcgacc gtgcccccat cgaaccatct gtatggccga aggtcatgct
     2641 gttgaattgt ttgccgcgcc gcgtggacag atagaattta cccgcgctgg cgcgccggtc
     2701 ggggggcttc atcggggtga attgggcgcg attttcgggc atgccggtaa agccgtgctt
     2761 gaaaagaaac ggtccgcccc attggaaatg atcgccctcc ttggtgagct tctcgatgcc
     2821 atgataaatc ggcatcaccc gagccatttc ttcacgaata ctttgagtat cgtcgaaggg
     2881 gaagagaagc tcgccattgg gcatcgcctt gcgcccgatc cgcatcggga tttcccattc
     2941 gggcagggtt tcgccgatgc tatggccggg aatctccggg ctgaaacgga ttcgcctttc
     3001 ggtgctggtc gaggttccgc caccgcgttg ttcatagcgg gtttgactcg gcaggatcag
     3061 gatcgattcc atagcgttga gcaacatgga ggtgttgagc agaatgtcct gatgtatccg
     3121 gacgggcacg cgctcgaggg ctcttgcgac gtagtttcga tccggcaaag cctccaataa
     3181 atttccaccg atagaataga gaaattgaag gtctccgcga cctgcggctt cgatcatgtg
     3241 cggcactctt aaacctggcc gcgccgggat cggatgcttc catagattgg aaaaacggcg
     3301 ggcgctggat tcatccaccg acaatccgcc gggaaattta tcgggttcca cgccgcaatc
     3361 tgcgccgccc tgagcgccgc tgggcccgcg catcggcata atgccgcatt tttcccggcc
     3421 gaacatgccg cgcgccagag ccagattgac gagcgctttg acgttatcga cgccgaactc
     3481 atgttgggtc aatccggtgc tgtaaacgaa cacggcagac tgcgcggtgc cgtagcgttg
     3541 ggcaaagtcg atcatttggt tgcgagtgac gcccgagagt tgctcgagca actcccacga
     3601 ttgttgttcg acggcagctc tcatttgctc aaacccggcg gtgtgggcgg caataaagcc
     3661 gtgatgcacc tgatctgcgg ccaggagcgc tttgagaatt ccattgatga acgcgatatc
     3721 cccgccgaca cgcacctggt aaaaatcatc catcagcttg gtgccgaaaa tcgcgctgga
     3781 tgccagcgcc ggtacccggt agcgttccag accgtactcg cgcatgggat tgacgacgat
     3841 gatgcgcgtg ccctgttgtt tggcgaggtg catgtatttg gtcgtcaccg gatgattgtt
     3901 ggccaagtcg gaaccgaaga tgacaagcat ctcggtgccg atgaaatccg caagcgagcc
     3961 ggtgggcgcg ccaacaccca aggtggcttt caaccccgaa accgtggcgg catgacaaag
     4021 gcgcgagcac agatcgacat tgttggtgcc gagcacccgc gccagttttt gaaagacata
     4081 gtaggcctca tttgtcagcc cgcgtgaagt tgcaaaaaag gccatctgat tagggggagc
     4141 attgcgaatc gatcgggtcg tcaattccac tgcttgatcc cagctgatcc ggttaaagcc
     4201 cttctctcct cgcctgcgaa gcatggggta agggatgcgt cccaaggatc ggagcttctc
     4261 cggtgcgagc cggcgaaggg cgccgacgtc gctcatgcgc gacggatcca gcgcgctcat
     4321 cgtgttaacc ttgagaagct tgaggcgtgt catgcacaga tgaacgcctt catgaacatt
     4381 atccttgagg ccgtacgggc ccaaagagca accgtcgcac acgccccgtt tcaagatatc
     4441 ccaggcgtac gggatttcgg ttttattctc ccagatcacg cccagcattt cgcggtaatg
     4501 tctcggctgg gtctggccga ccaaaccgaa cgggacccag cgcgcgaggc cggtgtcgtt
     4561 ggatgttacc ttagacatgg gtcttgcaca ttaaccctat gggtggcgca gctcgtcgag
     4621 aaccgcaaaa aacttttcgg gttcgctttt cgacacagct gttttgtaca aggcataccg
     4681 ttgatgagac gcccagccga tccactcttc gagtgtaacc gcgtcgccat cgtcgccgga
     4741 ccgttccaag accggtgccg gaacttgatc cgagatatcc cacaacggtt ccatgggcgg
     4801 gagctcttgc gccggcgcgc cgcaataccg gcgggagaga aatctcaagt agtctttgaa
     4861 aacttgtttc tcgtcttcgc aatccaccgg caagtggcag atggcgatcc gttcttcgat
     4921 atgaaaggcg agccactgct tgagagatag tttgatacct gtaagatcga gcttcatccg
     4981 cacatgcaaa ggaagacggt ccaaattcgg aaaaagatct ttttcgtagc ggaatcggta
     5041 aaacataatc tgcgagggag tttgcctttc gaccgggcaa aaagatattt tcctcttcat
     5101 tcatggaagt caaacacatt atgtatgtat tggattaaaa agacacgtaa atagccttgt
     5161 ctgaaccgct tagcaacagc gggacgggcc gtatcgccaa tggcgtttca aaacacggtg
     5221 attttctgca ttgcccgggg catgactcgg ttgagcttgc gtcagtgaga cagttaatga
     5281 acgaaggagt ttttaagacc aagtattcta tattaaaaat gttctggcgc cgcgccgcgg
     5341 aggcccggtt gcatttagaa ggccgccgga aaaaagttgg cagatactat gaatcgttac
     5401 agagaagtca tcttagctaa gaggaatacc gtacgatatg cagagtcgcc cacccgaagc
     5461 gaataatcca ccgccgccta tcgaaccgac aggattcttt accggaatca aaatacgtcc
     5521 ggtcatcatc ggtgtcgtcg tcgatatcgt cgccacgatg atgctgtcga ccctttatta
     5581 ctttatcttc gtagcgaagc agctaagcga cagcggcgca atgtccgagg aggcgatggc
     5641 tcagtactgg agttccagcg aaggcatcat ggccagtgtt gtgatcggca cgctttgtac
     5701 cggcatcggc ggcttctacg ccggccgcaa agccggcgca ttggagatga agcacggcgg
     5761 ccttgttggt ctcggttcga tcctgaccgg aatcatcctg caatcggccg gcgaagacgg
     5821 caccggactc aatcaatggc aaatgctgat cgcctatgcg tcggcgattc ccgccggagc
     5881 tttgggcggc tctgtggcgg agtgggcgcg cggctttgcg ccgatgaaac cgccggacgg
     5941 cggcactcgg cgccggcgtt aagcatgcta tgggcagcat cgaatacaga cgcgccggcc
     6001 cggatgatta tgccgaaatt gttcggctgc agcgggtcaa ttatatcggc aacctctcgg
     6061 aagaggagcg ccgggagggt tttctctccg ccgagttcag tttagagcag gtagcggcta
     6121 tcgccaccga tctcggcatc gccatcgtta ccatggacga tgaccttgcc gggtgcttgt
     6181 gcgggatcag gcgcgaattc gatcaccgct cgccggtggt tgcaaaaatg ctcgattcct
     6241 acgaccaagt gtggttcgaa gacaaaccgc tcagcgcctt caacagctat atctacggcc
     6301 ccgtgtgcct tgctcgtcag taccgccgcc gcggcctgct gcgcggcctc tatgactttc
     6361 aaaaaaaaga tttggccggc cgattcgaac ttggcgttgc cctggtctca catagcaatc
     6421 cacattcaat gcaggcgcat gtggaaggtt tgggaatgac caaaacgggc gaatttgagc
     6481 tcaacggcag cttattcgcg atcctggcgt tccgcatttg aacctactgg cggctgcaaa
     6541 aagaaatcat atgcttcgtt gcgcttattc gcctcctcag ttggagtact ggagtgatgg
     6601 actgatgggt tcgagacccc aatactccgt tgctccaaca ctccattccg tttgcccgcg
     6661 ccgtcttgcg gattggccag cgctctcata gaatcgcctc agtcgttgcc gttcctggtg
     6721 acggcgacaa tctcgaaagg agataaataa ataatggata aagacaccaa gaagaccgca
     6781 ttgcgcatga tcccgtatgg actttatgtt ctgactggtg aaggcaagga cggcaagatt
     6841 gccgcggcaa cagtcaactg ggtgacgcag actgcgtttg agccgccact ggtagtcgtc
     6901 ggcgtcaaag ccgactccgg cgctcatgcg attatcaagg aatccggaac tttcgcgtta
     6961 aatattctcg gcaaggatca aaaaggccag gcttatactt ttttcaaggc gctcgagcgc
     7021 gaaggcgata ccatcggcgg cgaaccattt cgcaagggaa gtcttggcgc gccgattttg
     7081 cagaatgctc cggcatttgt cgagtgccag cttgtagaaa ccatcgagaa gggcgatcat
     7141 tcgattttcg tcggtgaggt caaagatgcc ggggttgccg cgcaacctga gggacgccct
     7201 gacgatgcga cgctgacact taaagatctc ggcgacaaag ttttttacgg cggctagcag
     7261 cctgatgaaa aaggccacct gcttcgttgc gctcggtcgt ctcgcttcaa cgtacactag
     7321 gagtacgctt tcgctcgacg actcatcgcg cgtctcgcat ctagcctttt tgatcaggct
     7381 gccaacaaga aaataaaaac gatccgataa aggggaaatt atggctaaaa ttcgacacat
     7441 tgcttaccgc gcagaggacg
//
LOCUS       DASUVS010000402         2556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_616270, whole genome shotgun sequence.
ACCESSION   DASUVS010000402 DASUVS010000000
VERSION     DASUVS010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616270"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VF182_21550"
     CDS             <1..618
                     /locus_tag="VF182_21550"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6439717.1"
                     /translation="LTVHGAIQLLRETSANRTIFSMILLGGATSLLVGNAFQAQMPGF
                     AHDFGHDNQDWAYSVLLGANAAGAVLGGLLLEGKGLLKASPWNAAVCSILWCVSIIGF
                     AATDTYGLGVMLLFCAGLLNLAFMSMAQTLVQLRAPAQLRGRLIGLFNMSSNGLKAFS
                     GVTVGVVGGMIGIHWSLGISAMALLAVTIGLFAFTIPGRRSEEAV"
     gene            complement(675..1802)
                     /locus_tag="VF182_21555"
     CDS             complement(675..1802)
                     /locus_tag="VF182_21555"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6439718.1"
                     /translation="MKNGYSVMDSDMHVVEPCDLWQRYIDKKFADRAPIGLNRHKRDL
                     GVQVEGKVMPRAPEKPNPALRPIREKILNQRYPEEEARNFDNIAQLRAMDKEGLDVAI
                     LYPSRGLFVLAIDGLDPELAAAIAKAYNDWMSDFCQVAPQRMYGAAIVAPHDVPSAVE
                     ETRRIVNELGFKSILMRPNHVNGRRWSDSYYDPLWAECQSLGIPVGFHEAGRVFLPQP
                     ALSQFIPSFTMFNTLSFPMANMLACADMIYGGVMERFPKLKVAFLEGNCSWIPWLLWR
                     MGEYAELTGAAEHPELKLEPLEYFQRQCWGAVECDEVTAKNLPDFGLEDNIVFSTDYP
                     HLDVKYPHAVETFLKLPFSDRTKRKYLWENCARLYGFDHDG"
     gene            complement(2035..>2556)
                     /locus_tag="VF182_21560"
     CDS             complement(2035..>2556)
                     /locus_tag="VF182_21560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439719.1"
                     /translation="ILEKNGLNPDKDVTILALGRQDDLHLALKSARISAAIYSPPRSL
                     MLYRDGFHKLAFSGDYLPHYPIGGIGVTDDLIRKRRADIHSFLAATLTSIAYYRQNRS
                     EAIEFIAAELKMSDASLAEQMFDWHRTVLADEGRPSQTWINGVLDFTKKSLELSNPIS
                     ADQVFDFSFVDRR"
BASE COUNT          541 a          698 c          710 g          607 t
ORIGIN      
        1 cttacggtgc atggcgcgat ccaattgctg cgcgagactt cggccaatcg cacgattttc
       61 tcaatgatcc ttctgggcgg cgcgacctct cttttggtcg gcaacgcctt ccaagcgcaa
      121 atgccgggat tcgcccatga ctttggccat gacaaccagg attgggcgta cagcgtgctg
      181 ctcggcgcca acgccgccgg cgcggttctc ggcggcctgc tgttggaagg caaggggctg
      241 ctcaaagcga gcccgtggaa tgccgcggtc tgctcgattc tgtggtgcgt cagcatcatc
      301 ggattcgccg ccaccgatac ttatggcttg ggcgttatgc tgctgttttg cgcagggctg
      361 ttgaatctgg ctttcatgtc gatggcgcaa accctggtgc agctcagggc accggcgcaa
      421 ctgcgtggcc gactgatcgg gctattcaac atgtcgagca atgggctgaa ggcgtttagc
      481 ggagtgaccg tcggcgtggt cggcggcatg atcggcatcc actggtcatt gggcatcagc
      541 gcgatggcgc tgttggcagt caccattggg ctgtttgcgt tcacgatacc ggggcggagg
      601 agtgaagagg cggtttgacg gttctttgaa ttacgatgtt agtagataag acgacctttg
      661 tccccccatc agaattatcc gtcgtggtcg aagccgtaaa gccgcgcgca attttcccat
      721 aggtacttcc gcttggtccg atcgctgaac ggcagcttca agaaagtttc caccgcatgc
      781 ggatatttga catcgaggtg cggataatcg gttgagaaga cgatattgtc ttccaagccg
      841 aaatccggga gattctttgc cgtcacttca tcgcattcca ccgctcccca acactggcgt
      901 tgaaaatatt ccagcggttc gagcttgagc tcagggtgtt ccgccgctcc ggtgagctcg
      961 gcatattcgc ccatgcgcca taacagccag ggaatccacg agcagtttcc ttcgaggaat
     1021 gcaactttta atttcgggaa acgttccatg actccgccgt aaatcatgtc ggcgcaagcc
     1081 agcatgttgg ccatcggaaa cgacagcgta ttgaacatcg tgaagcttgg gatgaactgc
     1141 gacaacgcgg gttgcggcaa gaacacccgg cccgcctcat gaaagccgac gggaattccc
     1201 aaactctggc attctgccca cagcggatcg tagtaagagt cgctccacct ccgcccgttt
     1261 acatgattgg gccgcatcag aatgcttttg aatccaagct cgttaactat gcggcgggtt
     1321 tcttccaccg ccgaagggac atcgtggggc gcgacgatgg cggcgccata cattcgctgc
     1381 ggcgccactt ggcaaaaatc cgacatccag tcattgtaag ctttggcgat ggccgcggcg
     1441 agctcgggat ctaaaccgtc gatcgcgagc acgaacaaac ctcggctcgg gtacagaatc
     1501 gccacgtcga gaccctcttt atccatcgcc cgcaattgcg cgatattatc gaaattgcgc
     1561 gcctcttcct cggggtatcg ctggttgaga atcttctcac ggatgggccg cagcgccgga
     1621 ttgggctttt ccggcgcgcg gggcatgacc ttaccttcga cttgaacgcc gaggtcccgc
     1681 ttatgccggt taagaccgat gggcgcgcga tcagcgaatt ttttatcgat gtaccgttgc
     1741 cacaaatcgc acggctcaac gacgtgcata tcggaatcca tcacgctgta tccgttcttc
     1801 attctttgtc ctcgacagtc tgctgaaaag ctcataggcg ccggaaaaat gaattatgaa
     1861 atctgaaaac tgaattccgc tttcatcctt gtaattcacc cgcccttggc tctgagatct
     1921 ttttaagcag tcttcgttta ggtagcctaa atatccacgg catagcaaga taccactcag
     1981 atgagtttcc gacgttgaac ggttgaacct tgaacaaagc cggcgggaag ccggttatct
     2041 cctatcgaca aaactgaaat cgaatacttg atcggccgag atagggttgc ttagctccag
     2101 gctctttttg gtgaagtcga gtacgccatt gatccacgtc tggctaggac gcccctcatc
     2161 ggcaagcacg gtgcgatgcc agtcgaacat ctgctcggcc aacgatgcat cgctcatctt
     2221 tagctcggcg gcgataaatt caatcgcttc acttcgattc tggcgatagt aggcgatact
     2281 ggtaagcgtt gccgctaaga aagaatgaat gtcggcgcgt cgctttctta tcagatcatc
     2341 ggtgactccg atgcctccta tcggatagtg cggcaagtaa tcacccgaga acgcgagctt
     2401 gtgaaaaccg tctcggtaga gcatgaggct gcgtggcggg ctgtagatgg ctgccgagat
     2461 cctagcgctt ttgagggcga gatgaagatc atcctgtcgg ccgagcgcca gaatagtgac
     2521 gtccttgtcc gggttcaatc cattcttttc taggat
//
LOCUS       DASUVS010000403        13463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1051750, whole genome shotgun sequence.
ACCESSION   DASUVS010000403 DASUVS010000000
VERSION     DASUVS010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13463
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051750"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VF182_21565"
     CDS             <1..507
                     /locus_tag="VF182_21565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6439720.1"
                     /translation="RLDAKKMSIELAAVDIDDLVAHAQSQIEQLNRHNRLQVSWDVEP
                     HLPTIVTDVTKLEEILQNLIGNAFKFTPRGRIQLKIRNLHELSRVEFTVADTGIGIEP
                     HDIERIFAAFEQIREAHTGEYNGVGLGLNIVKKYLDLMKGDIRVESQPGEGTTFTFSV
                     PHSIGLDS"
     gene            complement(683..759)
                     /locus_tag="VF182_21570"
     tRNA            complement(683..759)
                     /locus_tag="VF182_21570"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(723..725),aa:Pro,seq:cgg)
     gene            complement(807..1466)
                     /gene="rpe"
                     /locus_tag="VF182_21575"
     CDS             complement(807..1466)
                     /gene="rpe"
                     /locus_tag="VF182_21575"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX6439721.1"
                     /translation="MHSLKIAPSILSADFSRLKDEIQTVEAAGADWLHIDVMDGHFVP
                     NITVGPVVVESVRKVTKMPLDVHLMITDPDKYAPEFIKAGADWVSIHPNTTQNLSESL
                     QKIRELGAKASIAVNPDIAIESVETCFPDIDMILMMTVFPGFAGQAFISDVLPKIEQV
                     RNKIEKEELPILIEADGGIKADNINLVVRAGAEVIVSGSGIFKTSSYSDTIRRMRQAV
                     G"
     gene            complement(1467..2828)
                     /gene="rsmB"
                     /locus_tag="VF182_21580"
     CDS             complement(1467..2828)
                     /gene="rsmB"
                     /locus_tag="VF182_21580"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEX6439722.1"
                     /translation="MTTSENSGNVRKQAVDILVRIELDKAYADILLDQALGKGSIPDK
                     DRSLLTEIVYGTLRWRGRIDSHLKQLTRRSFEETDPFIKNLLRITLYQISFLDRIPEY
                     AALNEAVNLAKMHSGSRAGGFVNGVFRNLIRMSRTVVPPDPDSCGVTALADYWSHPEW
                     LINEWIDYLGRAHVAPLLAANNRQAPRVLRVNVSRTDRETLLGTFKAHGIDAIPTKWS
                     PHGIVVHSQVPIERLSGYHEGFFQVQGEASQLVTYLLDPHPGETILDACAAPGGKTTH
                     IAERMNDKGQVTATDRSAKGIAKIDENAVRLGLKSIRTIHADMTKPVAAAQESTYDRI
                     LVDAPCSGLGTLRSHPEIKWNRGITDVVRLGELQRKILAQAASYLKPEGVLVYSTCTL
                     TAEENEKVVAEFLAYHRDFALDPAENYLPEQKELINGHYFMALPHKHDTDGFFAARLR
                     RVV"
     gene            complement(2815..3753)
                     /gene="fmt"
                     /locus_tag="VF182_21585"
     CDS             complement(2815..3753)
                     /gene="fmt"
                     /locus_tag="VF182_21585"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX6439723.1"
                     /translation="MASSWPIIFMGTPEIAAVTLLTLLEGSDPVVGVVTQPDRPTGRG
                     QQTAPSPVRRMAESRGIPVIAPTKIRDSGFLDTLRSWNPQIIVVVAFGRILPKAVLDL
                     APRGCLNVHYSLLPKYRGAAPVTWTIINGEEKGGVTTMQLVEQLDAGPIYLQEEIKLV
                     EHETTASLQAKLAPLGAKLLLKTLDGLKSNSLQRHDQDETRVIFAPMIRKEDGVIDWT
                     QPALTIERRVRAFTPWPSAYTHLNGKILKVHRANPIQTNRSGKPGEILRADTGGFWIA
                     TGNGTLSLEEVQLENKKRLPGVEFIKGARIAAGDHV"
     gene            complement(3808..4347)
                     /gene="def"
                     /locus_tag="VF182_21590"
     CDS             complement(3808..4347)
                     /gene="def"
                     /locus_tag="VF182_21590"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX6439724.1"
                     /translation="MALLEILKYPDSRLSKTALPVSNVTDATVQLINDMLETMYAAPG
                     VGLAATQVGVLQRIVVLDVDHENPRTNVYKLINPVITHSEGQLTWEEGCLSVVDFTAE
                     VRRAEQVQVVALDERGNERKIEAEGLLAVALQHEIDHLDGKLFIDRISRLKRDLYTRR
                     RKKMLRSGIEPPESAQVMI"
     gene            complement(4360..6645)
                     /gene="priA"
                     /locus_tag="VF182_21595"
     CDS             complement(4360..6645)
                     /gene="priA"
                     /locus_tag="VF182_21595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX6439725.1"
                     /translation="MSHQAGTFARVIIPSPVKEPLTYRIPQELRDAVTVGMRVLIPLG
                     NRKVSGVVFELISETPLQHIRDVISALDETPIVDELLLTLSQWASQYYVASIGEVLST
                     ILPPTSRMEIETIVVAKSETVQTNDVLGQKILAVLREQNGRASFKSLTRAFGETNISR
                     ALKRLQSAGDIEMRERLPVQRRKRRVEASQLSRPPCANLRFTLSTEQHAALAAIRARL
                     KTGGFATFLLHGVTGSGKTEVYLRAMEGVRDSGCQSLILIPEISLTPQLLNRVNARFN
                     GRVGVLHSGLTPAERWAQWWQIKRGDVDIVVGARSAVFAPVPNLGLVIVDEEHDGSYK
                     QDDGLRYNARDVAVMRGKLSACPVVLGSATPSLESYENCRHGRYELLEISQRVEQRPL
                     PRVEIFDLRNQFKSTSTAARNDAPNLSLKRKNAGERLLSDPLATAIKRNIDDNRQTLI
                     FLNRRGFANFLQCLRCGYVLRCSYCSVTLTLHLHRNVIRCHHCDFSRAVEKRCPDCGN
                     ETLAGVGAGTEQIEKTLHELVPEARIARMDRDTTGKRGSHADLIRRWESGDIDILIGT
                     QMITKGHDVSGVTLVGALLADMSLNLPDFRSAERTFQILSQVAGRSGRGNDPGRVIIQ
                     TYGPDHYAIQPLIEHDYKKFFAKEIEFRRALGYPPFGKLVNLRLDGPNPEHVEARAQA
                     LLKKIRAIQSGNSKYREHIEILGPAPSPIEKLRSRYRWQLLLKGKQIGPLIGLATAVR
                     ETFTRSRDVRLHIDVDPYNML"
     gene            7446..8534
                     /gene="pilM"
                     /locus_tag="VF182_21600"
     CDS             7446..8534
                     /gene="pilM"
                     /locus_tag="VF182_21600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEX6439726.1"
                     /translation="MSKLNLSSLASFGRNGGFVALDIGSSSIKMVETSIERTGYRVLS
                     LGVLPIPMDAIQNNVVVNTNLVADTIRRLVQENRVKSTQVVSAVPGRAVIMKRIEMPM
                     QEEAELEANIEFEANNVIPERMENVNLDYQVVNAMAGGSKLDVLLVAVKKEIVNSYTE
                     AIEAAGLKPVIMDVDYFAMENMYEANYSTETGPGMVGLIHIGARYTTVTLLHNSMSTF
                     TGDLPFGGDDFSDSLKRELNLSSEAAESAKITGLVEGKKGFDIEAMLKPTVENLAEEI
                     RRTVSLYGTVGTEEADGLKAIYLSGGSAQTLGLRAILEQQLAVPVRMAEPFRGFTVDK
                     KIDRHFITEDAPAFAVGAGLSIRRPGDK"
     gene            8531..9130
                     /locus_tag="VF182_21605"
     CDS             8531..9130
                     /locus_tag="VF182_21605"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX6439727.1"
                     /translation="MIRLNLLPVRELNAESDRRRDLAVAGVTLAATTLMLIAVYLYQS
                     AQLSNLANELPAIRKEVEVLNGKVKELGNLEAKIKELRSKHKVIEDLRGQKMGPTLVM
                     DSLAAAVPSSVWLVELKQSGGSLTINGLAMDNQTVAEFIKSLSQSGHFKNVELVETTQ
                     ADPKTGPYKKFSIQSAISYQIPATSASTPAQAEPKGSKS"
     gene            9127..9750
                     /locus_tag="VF182_21610"
     CDS             9127..9750
                     /locus_tag="VF182_21610"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX6439728.1"
                     /translation="MNAAIEKILDRPKGQKLGILVGLIILFSAAFYSFLYSPQADELT
                     KLNESIETAQNEKIIKQKRAANLPKLQKELGELDVRLKEAIAQLPSKKEIADLLSNVE
                     AKAQESGLDINLFRPRPENYKDFYAEIPVEINIKGTFHNVIMFFDAVGRLTRLINIDN
                     VGFKNPKLSGDQMILEFTTTATTYRFLEEAERKKIAEEKAAAAKAKK"
     gene            9790..10389
                     /locus_tag="VF182_21615"
     CDS             9790..10389
                     /locus_tag="VF182_21615"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEX6439729.1"
                     /translation="MIVVLALGSVFQFVIAQEQIQTPSQKTKEAVEKLVKTPATIGKS
                     LGVLTEAANAKLKQVLGDPQEFKTNAEPDDALLLQKKNPEPESPKFVRKGNRDPFRPP
                     SIQSRTGTRSRDNLSPLERFELGQLKVVGIVWDVKEPRAMIEDNAGLGYTILVGTRIG
                     VSEGRVKGIHRDQVVVEEFFEDISGKKKTREVSMKLSTD"
     gene            10418..12427
                     /gene="pilQ"
                     /locus_tag="VF182_21620"
     CDS             10418..12427
                     /gene="pilQ"
                     /locus_tag="VF182_21620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEX6439730.1"
                     /translation="MLCLNRRVQRIAQSRLVPALFLIVGIVQGAALSGCGPSTSKGPQ
                     VGQNQATAQTPTRSQPVNKEQPTPAGLLKLRDLSLVEERGQTTLSIKFSQPITEYRHF
                     PLSQPSRIVIDVLSDAKQAPLSESFRIDTHWVGTLRLTANDAGIRLASDITAATVPPY
                     VVTPEDGGLKIVIGLFNPDATGKRQAVLYRDGVRSDIRPAGQALSLKSSIEEGTSSLA
                     GQPKSEDKKYTGQKISLEFKDADIKNVFRLLAEVSGNNIMVTDDVSRKVTLRLIELPW
                     DQAMDLIVETNGLAKEEIGNVIRISTAGRFKSDRDALVAARRARDDLEPLQTVYYSIN
                     YAKVKELESKVKPLMSKRPDAALVIDERSNTIMLRDLQKTVEDVSTLVAKLDLRTAQV
                     LIESNLVETTPTFSRALGTEIDAIFNGRIVTSSRFLAGSPFAGSANTVAPPFPVPSTG
                     FRFGYTPANLNGFITAAENEGNAKIISRPSIVTLNNVPSTIKSERILRIALPSSTNIA
                     SGSGAAAGTAVATEKIPVGINLTVTPQVSSDGFVLMNIKVKSSSVANSSTVSGGTAGV
                     IPFDELNREAEANVLVRDGETIVIGGILKDIDSDSQSGIPYLKDIPVFGWLFRNWRFQ
                     KDFEELVVFITPRIAAAGSENLPSAEQLWRNQMERTKAEAATETH"
     gene            complement(12611..13354)
                     /locus_tag="VF182_21625"
     CDS             complement(12611..13354)
                     /locus_tag="VF182_21625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439731.1"
                     /translation="MNRELIVFYAAAVIVLLPLPASADYTIVLKNGRQFNVQNFREES
                     GAIKFYGFGGEISILKNQVQTIRPAGEANPATEPPRRSAPALVPENGVPAEKRTTTSP
                     QSARDSLRSEEKEITTPNVNDEDSYRQLIKKIDDQLKELRSRYATETRGNAGSEPFLF
                     TTEEAFRGHQEDLLSRLRDAQYKAQGLPAGVDAQSPPFSTNPPPAYSEKQKLLSSLRN
                     QIHELESERERVIAEMKRRDMDTGSLSSE"
BASE COUNT         3391 a         3312 c         3484 g         3276 t
ORIGIN      
        1 cggctcgacg ccaaaaaaat gtcaattgag ctggcggccg tcgatatcga cgatttagtc
       61 gctcacgcac aaagtcaaat tgaacaactt aatcgccaca atcgtctcca ggtgtcgtgg
      121 gatgtggaac cgcacttgcc cactatcgtc acggatgtaa ccaagctgga agagattttg
      181 caaaacttga tcggcaatgc cttcaagttt accccgcgag ggcggatcca actgaaaatt
      241 agaaatcttc acgagctcag ccgtgttgag tttactgtcg ctgacacggg gatcgggatt
      301 gaaccgcatg atatcgagag aatctttgcc gccttcgagc aaataaggga ggcgcacacg
      361 ggagaatata acggagtcgg tctcgggtta aacattgtga aaaaatatct agatctaatg
      421 aaaggcgaca ttcgcgtcga gagtcaacct ggcgaaggca caacattcac tttttcagtc
      481 cctcattcta ttgggctcga ttcttgagca agtaacagac aacggttgaa cgattgaaga
      541 cgtaggccgt gaatgcctta ccggcaattc caagaaaacc tcgtggacga tgttcccatg
      601 agagtgaaga gtcgcgcttc gagcgataag ggactctgag atcaataaca ggtgaacgac
      661 ccaggatctt gtggaaagtg aatggtcggg gcgagtggat ttgaaccacc ggcctcacgg
      721 tcccgaaccg tgcgctctac caagctgagc cacgccccga ctcggtataa gtaacacacg
      781 gaaagtagaa aacaacaggg attagatcag ccaaccgctt gacgcatgcg gcgaatggtg
      841 tcgctgtaac tagaggtctt gaaaatgccg gaaccggaga cgatcacttc ggcgccagct
      901 cgtacaacca agtttatatt gtctgctttg atccccccat ctgcttcgat caggattggc
      961 agttcttctt tttcaatctt gtttctcact tgttcgatct tgggcaaaac atcagaaatg
     1021 aaagcttgac ccgcaaatcc agggaacacc gtcatcatga gaatcatgtc aatgtcggga
     1081 aagcaggttt cgacgctttc aatggcaatg tcaggattca cagcaataga cgccttggcc
     1141 cccaattcac gaattttctg caagctttca gatagattct gagtcgtatt cgggtggatt
     1201 gatacccaat cagcacctgc ttttatgaac tcaggggcgt acttgtccgg atcggtgatc
     1261 atcaagtgaa cgtccaaagg catctttgtc acttttcgaa cggactcgac gaccacggga
     1321 cctacggtaa tgttcggtac gaagtgaccg tccatcacat cgatgtgaag ccaatcagcg
     1381 ccagcggctt caactgtttg aatttcatct ttcagccggc tgaaatcggc actaagtatg
     1441 gatggggcaa ttttcagtga atgcatctat acaacccttc gcaagcgcgc cgcgaaaaag
     1501 ccatcagtat catgcttatg aggcagcgcc ataaaataat ggccgttgat aagctccttc
     1561 tgctccggaa gataattttc cgcgggatct aaagcaaagt ctctgtggta ggcaagaaat
     1621 tcagccacaa ctttttcgtt ctcctcggcg gtgagcgtgc acgtcgagta tactaaaacg
     1681 ccctcaggtt tgagatagct ggccgcctgg gcgagaatct ttcgttgcaa ctcgcctaac
     1741 cggacgacat cggtgatgcc gcggttccat ttgatctccg gatgagaccg caaagtgcct
     1801 agaccgctac acggcgcatc gacgaggatg cgatcatacg ttgattcctg tgccgcagct
     1861 actggtttcg tcatatcggc atgtatcgta cggatcgact ttagccctag tcggacggca
     1921 ttctcatcaa tctttgcaat acctttggcc gaacgatccg tggctgtgac ttgtcccttg
     1981 tcattcatcc gctcggcgat gtgggtagtt ttaccccccg gagcggcgca cgcatcgaga
     2041 atcgtctcgc ctgggtgtgg atcaaggaga taggtaacca gctgtgacgc ttcgccttga
     2101 acttggaaga acccctcatg ataaccactt aaccgctcaa ttggcacctg cgagtgaaca
     2161 acgataccgt gtggtgacca cttggtcggt attgcgtcaa tgccatgcgc tttgaacgta
     2221 cccaacaatg tttcgcgatc agttcgagac acattgaccc gtaacaccct aggcgcttgg
     2281 cggttattcg ccgccagaag cggtgcaaca tgagccctcc ctaaataatc aatccactcg
     2341 ttgatcaacc actccggatg agaccagtaa tcggccagcg ccgtgacgcc gcaactgtct
     2401 ggatccggcg ggaccactgt tctgctcatg cgaatgaggt tacggaaaac tccgtttaca
     2461 aacccgccgg cacggctacc cgaatgcatc ttggctaggt ttacagcttc gttaagcgca
     2521 gcatactcgg gtatccggtc gagaaaacta atctggtaga gggtgatccg gagaagattt
     2581 ttaataaatg gatcggtttc ctcaaaagaa cggcgcgtga gctgctttaa atgggaatct
     2641 attctgccgc gccatcgcaa ggttccataa acgatttccg tcaacagaga tcgatctttg
     2701 tctgggatac tgccttttcc gagtgcctga tccaagagaa tgtcggcgta agctttatca
     2761 agctcaattc tgacgagtat atccacggct tgtttgcgga cattcccgct gttttcagac
     2821 gtggtcacct gccgcaatcc gcgctccttt tataaattcc accccgggaa gccgtttttt
     2881 attctccaat tgaacctctt caagagagag cgtgccgttt ccggttgcaa tccaaaaccc
     2941 gccggtatct gctctgagaa tctcaccagg tttccctgac ctattcgttt gaattggatt
     3001 tgcgcggtga acttttaaga tcttcccgtt taggtgagta tacgcagacg gccaaggagt
     3061 aaaggcgcgg acacggcgct caatcgtcag ggcaggttga gtccagtcga ttacgccgtc
     3121 ttcttttcta atcatcggtg caaagatcac acgagtttcg tcttgatcgt gccgctgaag
     3181 agagttgctc ttaagtccat ccagcgtttt cagcagtaac ttggctccta ggggcgccag
     3241 cttagcttgc aacgaagctg ttgtctcgtg ctcaacgagt ttgatttctt cctgaagata
     3301 gatcggtccg gcatcaagtt gttcaaccag ctgcatggtg gtgacgccgc ctttttcttc
     3361 tccattaatg atcgtccatg tgaccggcgc tgcaccgcga tatttgggga gaagagagta
     3421 gtgtacgttt aaacatccac gtggtgcgag atccaacact gctttgggta gaatgcgtcc
     3481 aaaggctaca accacaataa tttgcggatt ccacgatctc agcgtatcga gaaaaccgct
     3541 gtcgcggatt tttgtgggtg caatcacagg aatgcctcga ctctcagcca tacggcgaac
     3601 cggagacggc gcggtttgtt gtcctctgcc cgtaggacgg tcgggctggg tgacgacgcc
     3661 gacaacaggg tcggaaccct cgagcaacgt tagcaaggtg acggcggcga tctccggagt
     3721 tcccataaat ataatgggcc aagaggaagc catgtaagat tgcctgagag actctgctag
     3781 aatctggctg ttatttgggc aagcagttta gatcatgacc tgtgccgatt cgggtggctc
     3841 gatcccgctg cgcaacatct ttttgcggcg acgtgtgtag aggtcgcgct ttaatcgact
     3901 aatacgatct ataaacagtt ttccatccaa atgatcgatt tcatgttgaa gcgccacagc
     3961 aagcaaacct tccgcctcta ttttccgctc attgccacgt tcgtctagcg cgacgacttg
     4021 tacttgttcc gcacggcgta cttcggccgt aaagtctacg acgcttaagc agccttcctc
     4081 ccaggtcagt tgtccctcgg agtgagtgat gacaggattg attaatttgt aaacgttagt
     4141 tcgcggattt tcgtggtcca cgtccagaac cacgatccgc tgtagtactc cgacctgtgt
     4201 cgcagctaaa ccaacaccgg gtgcggcgta catggtctca agcatgtcat tgataagttg
     4261 aacagtggca tcggtaacat tcgacaccgg aagtgcggtt tttgaaagcc gcgaatcggg
     4321 atatttgaga atttctaaaa gagccataac ctattatttt cataacatat tatagggatc
     4381 gacatcaatg tgcaggcgca cgtcccgtga tcttgtaaac gtctcgcgaa cggcagtggc
     4441 tagcccaatc aaagggccaa tttgcttgcc tttgagcaac agctgccagc gataacgact
     4501 ccgcaatttc tcgatgggtg aaggagccgg gccgagaatt tcaatgtgct cccggtactt
     4561 tgaattgccg ctttggattg cgcggatttt cttgagaagc gcttgggccc gcgcttccac
     4621 atgctcggga ttgggtccgt ccaagcgcaa atttaccagt ttgccgaacg gaggatagcc
     4681 caacgcccga cgaaactcga tttccttcgc gaaaaacttt ttgtagtcat gctcaattag
     4741 cggttggatt gcataatggt caggtccata ggtctgaatg atcactcgtc cgggatcatt
     4801 ccctcgtccg gatctgcccg caacctggct gagtatttga aacgtccgtt cagcgctccg
     4861 aaagtccggc agattcaaag acatatcggc aagcagcgct ccgactaacg ttactccgga
     4921 aacatcgtgt cctttggtga tcatttgggt tccgataagt atgtcgatat cgccgctctc
     4981 ccaccttcga atcaaatcgg cgtgcgagcc gcgtttgccg gtggtatctc tgtccatacg
     5041 cgcaattcgc gcttcaggca cgagctcatg gagagtcttc tcgatctgtt cggttcccgc
     5101 gccgacaccg gcaagggttt cattgccgca atccgggcaa cgtttttcta ccgctctact
     5161 aaaatcgcaa tgatgacagc gaatgacatt gcggtgtaaa tgtaaggtca acgtgacgct
     5221 gcaataagag cagcgcaaaa cataaccgca gcgcaggcac tgtaggaaat ttgcgaatcc
     5281 tcgccgattc aaaaagatca gcgtctggcg gttatcgtca atatttcgtt tgatcgcagt
     5341 tgcaagcgga tctgacagta atcgttctcc agcatttttt cgcttaagcg ataggttcgg
     5401 cgcatcattg cgggcggccg tactcgtgga tttgaactga ttgcggagat cgaagatttc
     5461 aacacgcggc agcgggcgct gctctacccg ctgggaaatc tccaacaatt cgtaccggcc
     5521 atggcggcaa ttttcatagc tctccaatga cggtgtggcc gagcctagaa ctacgggaca
     5581 agcagaaagc ttgccgcgca tgacagccac gtccctggca ttgtagcgca aaccgtcatc
     5641 ttgcttatag gacccgtcat gttcctcgtc gacaataacc aagccgagat ttggaactgg
     5701 cgcaaacacc gccgagcggg cgcccacaac aatgtcgacg tcacctcgtt tgatctgcca
     5761 ccactgcgcc catcgctcgg caggtgtcaa cccactgtgt aggacgccga ccctgccgtt
     5821 aaaccgtgcg tttactcgat tcagaagctg aggagttaat gagatttccg gaatcaggat
     5881 gagactttgg cagccgctgt cccgcacccc ctccatggca cgtaagtaaa cttcagtttt
     5941 tccgctgcca gtcacgccgt gcaagagaaa agtcgcgaat ccgccggttt tcaggcgagc
     6001 tctgatggcg gctagcgctg catgttgttc cgtgctgagc gtgaaccgta ggttcgcaca
     6061 tggtggtcgg gacagttgag aggcttccac ccggcgtttc cttcgctgca ccggcaagcg
     6121 ttcgcgcatt tcaatgtcgc cggcggattg aagtcgttta agtgcacggg agatattcgt
     6181 ttcgccaaag gctctcgtca aactcttgaa gctcgcacgc ccattttgct cccgcagcac
     6241 agctaaaatt ttttgcccta gaacatcatt ggtctgcacc gtttctgatt ttgcgacaac
     6301 gatggtctcg atttccatac gagaggtggg tggtagtatc gtgctcagta cttcgccaat
     6361 agatgctaca taatactggc tcgcccactg gctgagggtt aatagcagtt catcaacaat
     6421 gggagtctca tccaaagcag agattacatc cctgatgtgc tgcaacggcg tttcggagat
     6481 aagctcaaac accacccctg agaccttgcg gttacctagc ggaatcaaca cccgcatgcc
     6541 gacggttaca gcatctctga gttcttgggg aatacggtag gtgagaggct ctttaacagg
     6601 tgacgggatg atgacgcggg cgaaggtccc cgcttgatgg ctcatcgttc gactaataac
     6661 ttcggattgt tcaaaaggca acttgcgatg ctcacgaacg aacagcaggc ccaattccga
     6721 tgctgtcgga agtattgtag tcaagcgaaa ttggcattga caatccaatg actggtaatg
     6781 aaaacgcaag aatcatcgat tagatcatgc gcagttacct tcagaatcgt ttactatggt
     6841 cagtatcatg cctccaacga tctcattcgg ttttcacccc agtttggcgt actctacgca
     6901 attctgtcca taattgtcca actcggcaca ttatggcttt gatgaagacc gaagagtttg
     6961 aatgatggtt tggtatctac cggaaatgta gcgtaaatcc gctggcataa ttctggcttt
     7021 acgcgtcttc acctccacac ccgtgggcgt atcaataact gcgaactcaa gacgccaagg
     7081 ggcgcataaa gaagttgcca ggctaataac aggcggatgg caactcaaat atttcggtga
     7141 cttaggtagc ccgataaaga aggaaatggc ttagatcccg tgcccggtta tcaactatcc
     7201 acgacgaact ccgaaatgta acgttgtgat gaattgttga tttgtaatcg actgggatga
     7261 gctcgcgtac gaagtgtgct ttgcgtatct acattgccca acgagcgtaa gtaaggcaat
     7321 tggagctagg ctcgcctgcg agggctgtat gtctaggatg gttcaatttg atggagaaga
     7381 attcttagct tgaaaactca gcgcggcttt ttagctgaag gtaatagccc agtatgagtt
     7441 cgttcttgag caaactaaat ctaagttcct tggcatcgtt cggcaggaac ggtggttttg
     7501 ttgcgctcga tattgggtcg agctcgatta aaatggttga aacctcgatc gaacgaactg
     7561 gttatcgagt gttgagctta ggtgtgcttc cgattccgat ggatgccatt cagaacaacg
     7621 tggtggtcaa cactaacttg gtagccgata cgatccggcg tcttgttcaa gaaaatcgag
     7681 tgaagtcaac tcaggtcgtg tctgccgtgc ccggcagggc tgtcattatg aagcgaattg
     7741 aaatgccgat gcaggaagaa gccgaattgg aagccaatat tgagtttgaa gctaacaatg
     7801 tgatcccaga gagaatggag aatgtcaatc tagattatca agtggttaac gcaatggccg
     7861 gcgggagcaa gttggatgtt ttgctagttg ccgtcaaaaa agaaattgtc aacagctaca
     7921 ccgaggctat cgaggcagcc gggctcaagc cggtgatcat ggatgtggat tatttcgcca
     7981 tggaaaatat gtacgaagca aactattcga cggagaccgg ccccggaatg gttggcctta
     8041 tccacatcgg cgcacggtat actactgtaa ctttgctgca caatagcatg tcgaccttta
     8101 ccggtgatct gccattcggg ggagacgatt ttagcgatag cttgaaaaga gaattgaacc
     8161 tgtcttcgga agcggccgag tcggcaaaga tcacgggctt ggttgaagga aagaagggtt
     8221 tcgatattga agccatgctc aaacccaccg tagaaaatct agctgaagaa attcgacgaa
     8281 ccgtcagttt atacggcact gttggaacag aggaagctga tggcttgaaa gctatttacc
     8341 taagcggcgg cagtgctcag acgctcggat tgcgggctat cttggagcag caattggccg
     8401 tgccagtgcg aatggctgaa ccatttcgcg gattcactgt ggataagaaa atcgacagac
     8461 atttcatcac cgaagacgcg cccgcattcg cggtgggcgc tgggttatct atccggagac
     8521 ctggggacaa gtgattcggc ttaatctact gccggttcgt gaacttaacg cagagtcgga
     8581 tcgccgcagg gatctcgctg tagccggagt caccttagca gctaccacgt tgatgctgat
     8641 cgccgtttat ttatatcaat cagcgcaact gtcgaacttg gcgaacgagc ttcctgccat
     8701 tcggaaggaa gtggaggttc taaacggcaa ggtcaaagaa ctcggcaatc tggaagccaa
     8761 gataaaggag ctaagaagca agcataaagt aatcgaggac ctgcgcggcc aaaagatggg
     8821 tccaaccctg gtcatggaca gcctcgcggc agctgttccg tcaagcgttt ggttagtcga
     8881 actgaaacag agtggcggaa gcctcacgat caacgggctg gccatggaca atcagaccgt
     8941 cgccgaattt atcaaatcat tatcgcagtc cggtcatttc aagaacgttg aattagtaga
     9001 aacaacccag gcagatccga agacgggacc ctacaaaaag ttctccattc aatcagccat
     9061 ttcttatcag attccagcga ctagcgcgtc gactcctgca caggcggagc ccaaaggaag
     9121 caagagttga acgccgccat agagaaaatc ctagaccggc ccaaaggcca gaagctcggt
     9181 attttggtcg ggttgattat tcttttttca gcagcttttt actcattcct ttatagtccg
     9241 caggccgacg aacttaccaa gttgaacgaa agcatcgaga cggcacaaaa tgaaaaaatc
     9301 attaaacaga agcgagcggc taatttgccg aagctacaaa aagaattggg tgaactggat
     9361 gtcagattaa aagaagcgat agcccaactg ccaagcaaga aagaaattgc ggatctttta
     9421 tctaatgttg aggcaaaagc gcaagagtcc ggactcgaca tcaacctgtt tcgtccccgg
     9481 cctgaaaact acaaggattt ctatgcagaa attccggtgg agataaacat caaagggacg
     9541 tttcacaatg tgattatgtt ttttgatgct gtcggaagac ttacgcggtt gattaacatc
     9601 gataatgttg ggttcaagaa tcctaaattg tccggcgatc agatgattct cgaatttaca
     9661 acgacggcga ccacctaccg tttcctggaa gaggcggagc gaaaaaagat tgccgaagaa
     9721 aaggctgccg cggcgaaggc aaaaaagtaa cccatcatgc gagccttgtt tagattctct
     9781 ttgcatcttg tgatcgtagt cttagcgcta ggttcagtat tccagtttgt tatcgctcag
     9841 gagcagatcc agactccgtc ccaaaaaacc aaagaggctg tggaaaaatt ggtaaagacg
     9901 ccggccacga tcggcaaaag tctcggggta cttactgaag cggcgaacgc aaaactgaag
     9961 caggtccttg gtgacccaca ggaatttaaa acgaacgcgg aacccgatga cgccctgctc
    10021 ttgcaaaaga agaatcccga gcctgagtcg ccgaagtttg ttcggaaagg aaatcgcgat
    10081 ccttttcggc cgcccagcat tcaatcgagg accggtaccc ggtcccgaga caatttatct
    10141 cctctcgagc gctttgagtt gggacaactc aaggtggttg ggattgtttg ggacgtcaaa
    10201 gagccgcggg cgatgataga agacaacgca gggcttggtt acaccattct agtgggcacg
    10261 cgcataggcg ttagcgaagg cagagtgaaa ggaatccatc gcgaccaggt ggttgtcgag
    10321 gaattcttcg aagatatctc tggcaagaaa aaaacccgcg aagtctccat gaaactatca
    10381 actgattaag cgcaatcact aaggagaaag taaattcatg ttgtgtctga atcggcgggt
    10441 tcagagaatc gcccaaagca gattggttcc tgccctgttc ctcatcgtcg gaattgttca
    10501 aggcgcggca ttatccggtt gtgggcctag cacgagtaag ggccctcaag tcggccaaaa
    10561 ccaagccacg gctcaaacac cgacccgatc gcaacctgtc aataaagagc aaccgacccc
    10621 cgctgggctg ctcaagctac gggacctttc gctcgttgaa gagcgtggcc aaacaacatt
    10681 atcgattaaa ttttcgcagc ccataactga atatcggcat tttcctctct cacagccgtc
    10741 acgaattgtt atcgatgtat tgagcgacgc caaacaggcc ccgctcagtg agagctttcg
    10801 catcgacacg cactgggtag gaacgttacg cctgacagct aatgacgctg gcatccggtt
    10861 ggccagcgat atcacggccg ctacagtgcc gccttatgta gtcacccccg aagacggtgg
    10921 tctgaaaata gtgatcggtc tttttaaccc cgatgcgacg ggaaaaaggc aagcggttct
    10981 ctacagagat ggagtacgtt cagacatccg gccagccggg caggcgctat cgttaaagag
    11041 ctcgattgag gagggtacat cttctctggc gggccagcct aagtcggagg ataagaaata
    11101 tactggccag aagatttcgc tggagtttaa ggatgccgac ataaagaacg tttttcggct
    11161 tctggccgag gttagcggta ataacattat ggttacggat gacgtcagtc gcaaagttac
    11221 ccttcgtctg attgaacttc cgtgggatca agcgatggac ctaatcgttg aaaccaatgg
    11281 cctggctaaa gaagagatcg gcaacgtgat ccgaatttcc acagctggcc gattcaagtc
    11341 tgatagggat gcgctagtcg ctgcgaggag agccagagat gacctggagc cgctacagac
    11401 agtctactac agcatcaact atgctaaggt gaaagagctt gagtccaagg tgaagccgct
    11461 catgagcaag cgtcccgatg ccgccttggt cattgatgag cgaagcaata cgattatgtt
    11521 acgggatctg caaaagacgg tagaagatgt cagtactctc gtcgcaaaat tagatctgcg
    11581 aacggctcaa gttctgattg aatcaaacct agtggagacg acgccgacgt tttcgagagc
    11641 gttaggaact gaaatcgatg ccatatttaa tgggcgcatc gtcacaagtt caagattttt
    11701 ggccggtagt ccctttgctg gaagcgctaa cacagttgct ccccccttcc cggtgccgag
    11761 cacgggtttc cggttcgggt atacgccggc taacttgaac ggctttatca ccgcggctga
    11821 gaacgaagga aacgccaaaa ttatttcgcg accctcaata gtcactctca acaacgtgcc
    11881 gtcaacaatc aaaagcgaga ggattctgcg aattgcgctt cctagctcga ctaatatagc
    11941 cagtggcagt ggtgcagccg ccggtaccgc agtggcaacg gaaaagattc ccgtcggcat
    12001 caatctcact gtcacccctc aggtttcgag cgacggcttc gttttgatga atatcaaagt
    12061 gaagtcgagt tctgtcgcca attcgtcaac ggtttccggg ggaacggcag gtgtgattcc
    12121 ctttgacgag cttaatcgtg aggcagaagc aaatgtgctt gtccgagatg gtgagaccat
    12181 tgtaatcggc ggaatactga aggacatcga ctccgattct caaagtggca tcccctatct
    12241 gaaagacatt cctgtcttcg ggtggctctt tagaaattgg cgttttcaaa aagactttga
    12301 agaactcgtg gtctttatca ccccgaggat tgctgcagct ggttcagaaa acctgccgtc
    12361 agccgagcag ctttggcgca accaaatgga aagaaccaaa gcagaagcag caactgaaac
    12421 gcactaaccc tgcggcacca tcccggtaag atacggcttt gtggaaggag agacttgatc
    12481 gaacaaagtc gcctacttta gaagtgctgc tcgggtaagc tggagtgacg aaggttttct
    12541 acagttagtt tgcgagcggt catcgcttga cagaggtttt gtgatcggct gcggtataga
    12601 tgctctttga ttattctgaa gacagtgagc cagtgtccat gtcgcgtctt ttcatttcgg
    12661 caatcacgcg ctcgcgttcg ctttcgagct cgtgtatttg atttcttaaa ctactaagca
    12721 atttctgctt ttccgagtag gccggtgggg gattcgtaga aaatggaggg gattgtgcgt
    12781 cgactccagc gggcagtccc tgcgccttat attgagcgtc tctcaatcgc gatagtagat
    12841 cctcctggtg acctctaaac gcttcttcgg ttgtgaataa gaacggttcg gatcccgcgt
    12901 ttccacgggt ttccgtagcg tatcgacttc taagttcttt tagctggtca tcaattttct
    12961 tgatcaattg ccgataggag tcttcatcgt ttacgttcgg tgtagttatt tctttctctt
    13021 cgctgcgtag gctatctctc gcgctttgtg gggaagtagt cgtcctcttt tcagcaggca
    13081 ctccattttc ggggaccaat gcaggcgcgg accgtcttgg tggctccgta gccggattgg
    13141 cttcgccggc cggcctgatc gtctggacct gattcttgag aatagaaatt tcgccgccaa
    13201 aaccgtaaaa ctttattgca ccagactctt cacggaagtt ttgtacattg aattggcgac
    13261 cattcttcaa gactatcgtg taatccgcgg atgcaggaag gggtaataac acaatgactg
    13321 cggcggcgta aaaaacgatc aattcacgat tcataggtgg taaatatatc acggtaggac
    13381 gaagccagaa atggatttta gaagtttgtc attggcatgg ggcttaacgg gagcaacagg
    13441 tagatgtaac cctctccgtc ggc
//
LOCUS       DASUVS010000404        14251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1491147, whole genome shotgun sequence.
ACCESSION   DASUVS010000404 DASUVS010000000
VERSION     DASUVS010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14251
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491147"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="VF182_21630"
     CDS             complement(<1..1219)
                     /locus_tag="VF182_21630"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6439732.1"
                     /translation="MNEPAPPDSEASVPRSEKLKEVLNACKRMTSVGNIMSLLDLITT
                     EAAELLQADRASIFLLDREKCELWSVITHEKEKRIVLFDARLGIAGAAAMTGQTINVT
                     DAYEHPLFFKEVDQKIGYRTKTLLAVPIMNSKGEVIGVGEAINKKVGIFNEDDAEILR
                     SLAAHVCDATKTTQIDRVLPSQQDPDAGKEIGGEFSTRNIIGMSHRIQSVVRLIDQIR
                     DSSVDVLIQGESGTGKELVAKALHYNSPRARYPFVAVNCAALPESIIEAELFGIERGI
                     ATGVDRRIGKFEEAQAGTLFLDEIGDLSLPSQAKILRVLQERSVTRVGGRTALPLDLR
                     IIAATNKDLEREIKDGHFRVDLYYRLKVIHIETPALREVPEDIPLLANHFLEKYTKLM
                     KTEPKQFTPAALEC"
     gene            complement(1496..2485)
                     /locus_tag="VF182_21635"
     CDS             complement(1496..2485)
                     /locus_tag="VF182_21635"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX6439733.1"
                     /translation="MRMIVIGTGPIGGIIGGRLARAGHDVTFVDVDKEHVEAIRANGL
                     QVDVPDGAFNVKVKIVFPQEIHGKFDIGFIAVRSNYTPDALATVLPALNDDGLLVSMQ
                     NGINPPLLQDKVGADRTVGIAIRMGCRRVAPGHVQTATRGHLYIGHLHGRTTPQLSRL
                     HKLLDPVIDTEISDNILGVLWSKLTYTCLGFFGSLADFALATSCAGDANRRLLADFLG
                     EVVAVGTVAGARFIRLAEYNPPDLHPSIPIERRLAIINEMAKSWKDDDRKGPLRQIQK
                     NIPTEVDFTLGYVVREGESMNLPVPLCRRVLTMIHELESGKRQLGIGNYSKLR"
     gene            2748..3827
                     /locus_tag="VF182_21640"
     CDS             2748..3827
                     /locus_tag="VF182_21640"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX6439734.1"
                     /translation="MALVVFTAEHMRDQERLRNAVSHASNHAVVVGSSAAGILTGDGE
                     IEGSHGIAVLVLASDQLQAKAFLFESLRGHDAEAGVKIAQLARPVLDSNLLTLLMPDS
                     YNGQAQEMLHAMQHEIGYSPVIGAGSSESGVVGATFQLCKDQVASNAVTGLHLLGSFE
                     SSIDITQGCQPITEPLAITRADGNIIYEIDHRPAFEVFTSVLKGPLLEDLRRALMYVF
                     VGLPADRGRNTVGGGEYLVRNIIGLDSEKKIIGVADQVSEGQAMIFTLRDGQRARDDL
                     GQMLKRQAQRLAGKKPAFGVYFNCCARGASLYGIPGIDSAYIRQILGEFPLIGMFGGY
                     ELAPLGGANHLFAYTGVLALIAEGN"
     gene            complement(3912..5738)
                     /locus_tag="VF182_21645"
     CDS             complement(3912..5738)
                     /locus_tag="VF182_21645"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6439735.1"
                     /translation="MRIHRYTYWDGTQQVLFPTTEDLLKHLSDNLLEEEGVRRALRDL
                     MRRGLTSEDGQRSVKGMRDFLRQADEKRRELLNKYSPDSFKLSPEETQQLSDKLNSLA
                     EKLETYHDKMRNFMERLSGRYADSMDEMNRKMQDAYQRYQELQQRLKNQIADRSSQQS
                     SNPGGQDYQGMMNMLDRMNKLLEDENFLKNLPNMLDAAVDDLDNMLDNLDSLTQEQLQ
                     QLSDMMNQMQQLEQLMNQYPFQGSQRMGMGEAGQILGQLRGLEKFLRWGQRGMGDPSE
                     LDLDEIRNMLGEDAYEQLKYLKGVEQMLEEEGYIIRTGHGMRLTPKGMRKIGDKALRE
                     IFHMLNKNRWGDHSTSARGSQGEQLEQTKPYEFGDPLNVNISETLMNALETGNGSVPV
                     RIRPEDFSVHRNEFSTDSETVLCIDVSYSMLMNDALHAGKKVALALHRLIETKFPQDK
                     LHLVAFRSNAKIIRAEDLPSIVSLTYFMEHGTDIKEALRFARQLLGSNRSANRQIILI
                     TDGEPTAATLDQGGRLHSGWGSAMLHGRIVRETLKEVRRCTQSGIKINTFMLGADFYR
                     QGFVDQLSRLNAGRVFYTSPDQMGNYIVVDYLANKRKRIGSR"
     gene            5883..6995
                     /locus_tag="VF182_21650"
     CDS             5883..6995
                     /locus_tag="VF182_21650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6439736.1"
                     /translation="MISSDCVIIGAGFAGAATAYHLARRGLVDITLLEQEVIPGFHSS
                     GRNAAMIRQCVSEPALFDLTRDGAAFLRNLPDDWPEPVQFKQNGSLLLASADGWKKLQ
                     KEAELGRSLGVDIELWTPSEARRHVAVLENAQFDGAVWCATDGVVDIHGLLSGYLKYA
                     TAHGLQVHYGADVRAIRRTADGVFEVRTKKQMLTTRMIVNATGAWANLVAEMAGASAL
                     PLRPCRRHLFTTPPLAWIDSHWPFVWDVTHDIYFRPEGEGLLLCACDQQELSPGDPPL
                     DPMIGEVLAEKIQRYMPALEGVSIHKYWAGFRTISSDNRFVIGWDPNLEGFFWVAGLG
                     GHGVTTSSAVGALAADLIIAGDGKKVEAFSPSRFKT"
     gene            complement(7989..8642)
                     /locus_tag="VF182_21655"
     CDS             complement(7989..8642)
                     /locus_tag="VF182_21655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439737.1"
                     /translation="MLKDIRSFLMDKKLIVLCFALAAATPASTPPAEAHDGRLDQFGC
                     HLDQNQKYYHCHEGPYKRLSFDSKTQMIERLRNQYIALGRTWPYADAINTLDQPAAIV
                     ETTLEPQGVAFDEAKQARPGNRSQSSESGQRIARKEMVKPTNRQTSQAHPKPEITSAK
                     QTTPKQSETPRKRTEPELKVWVTQIRADGRPVFQSREGERFFLDDSGNKILIERRES"
     gene            8993..10042
                     /locus_tag="VF182_21660"
     CDS             8993..10042
                     /locus_tag="VF182_21660"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX6439738.1"
                     /translation="MTKDPPTHMSSLMGQSTPDATLRSSPDTVLWGYIAANLPPALSV
                     KSGAIVEVEALSHQGLTTQNDPENFFANYGIPGGEVLPDAKAVFSGVDRPKGASVHIL
                     TGPIYIDGAEPGDTLEVRVLDIRFRVPYGVNNTGPGKGVLPQLLKEQSARLIRLDLER
                     RVALFSSDIEIPLNPFMGIMAVSPPTSLGMVSSTPPGAWGGNMDLKFLGIGSSLYLPV
                     FNKGAQFFTGDGHAVQGDGEVDGGAIEISLAPKLQFLLHKGKRIKLPRVETASDYLLT
                     GLSIDLNEAARIALQETVEFLQAEKNLTAADAYALSSLAVDLGIGEAVDIVNLVYAKI
                     PKRIFRSNPPFWACT"
     gene            complement(10525..11544)
                     /locus_tag="VF182_21665"
     CDS             complement(10525..11544)
                     /locus_tag="VF182_21665"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6439739.1"
                     /translation="MFESIEMKLKLVLSISLMVLCFSNSLLAQTLDLETAKKEGRIVV
                     YGTTIPNVMAPIHAGFEKRYGVKVDYWRASATAVTDRAITEWRAGKPGFDVLFAINGT
                     ITLLKRENALAKYIPPAAAKFPAHLKDKDGVLTAFRHTPISILYNTELVRPGDLPKDF
                     DDLLNPKWQSKIAMPDPSRHTSTAQFLWNMQKVKGEKWLDYARALAKQKPFLLESFAP
                     VPTALVKGEAQLGITYAQYITQVKGPLSHVVFDKVLTDSTDLALSAKAAAPNAAKLYI
                     DYLCTAEVQKIIADSGDFPIAPGIYPNLKDAEKLVAHAVFMENPSDEQFSKLKEDFRK
                     IFLTQ"
     gene            complement(11753..12679)
                     /locus_tag="VF182_21670"
     CDS             complement(11753..12679)
                     /locus_tag="VF182_21670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX6439740.1"
                     /translation="MDSQLFLWIVFNVFVLGVLALDLLVLHRKAHAVSLREALVWSCV
                     WVSLALIFAVGINFFRGGEKALEFLAGYVIEWSLSVDNLFVFLVIFSFFSVPSSYQHR
                     VLFWGILGALVLRAGFIATGTALLSNFHWMIYVFGGFLVFTGIKLLFVGDEKVEPEKN
                     PAVRLVRRFVNVTPDYQGQRFFVRKDQKLWATPLFLVLVVVETTDVIFAVDSIPAIFA
                     ITLDPFIVYTSNVFAILGLRALFFLLAGVMEMFRYLRVGLAFVLCFVGAKMLIAEFYK
                     IPIGISLAVVGGILLISILASVLKQQQEDKAL"
     gene            complement(13259..14233)
                     /locus_tag="VF182_21675"
     CDS             complement(13259..14233)
                     /locus_tag="VF182_21675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX6439741.1"
                     /translation="MESERLFLWIGFNLFVLGMLALDLGVFHRKAHTISIKEASIWSV
                     VWVCLAMIFNVGIYYAWGSEAALEFLTGYVIEKSLSVDNLFVFLMIFQYFNTPSQYQH
                     RVLFWGILGALVLRAVFIATGSALLSNFHWMIYVFGGFLVVTGIKMYLQGDEKIAPER
                     NPVVRLFERFVPVIRNYDGQKFFVRREGKTYATLLMLVLIVVETTDVIFAVDSIPAIF
                     AITNDPFIIYTSNVFAILGLRALYFMLAGVMEMFVYLKVGLSVVLCFVGAKMMLVDIY
                     KIPIGVSLGIIGSVLLLSIIASWLIKPKDPKPPHVQEPVKADLSGDEA"
BASE COUNT         3291 a         3671 c         3783 g         3506 t
ORIGIN      
        1 ggcactccaa ggccgccggt gtaaactgct ttggctcggt cttcatcaat ttagtgtatt
       61 tctctaggaa atggtttgcc aggagcggaa tgtcctcagg tacttcccgt aaggctggag
      121 tttcgatgtg gatcactttc aagcgatagt aaagatcaac gcgaaaatgc ccatccttga
      181 tttcacgttc cagatctttg ttggtggcgg cgataatgcg cagatccagg ggtagcgccg
      241 ttcggccgcc cacgcgcgtc acggatcgtt cttggaggac gcgcagtatc ttggcttgtg
      301 acggcaggct gagatcgccg atttcgtcca agaatagcgt gccggcctga gcctcttcaa
      361 atttaccgat gcgccggtcc acgcccgtgg cgatgcctcg ttcaatgccg aagagttccg
      421 cttcgatgat actctccggc agagccgcgc aattcactgc aacgaagggg tatcttgccc
      481 gcgggctatt gtagtgcaaa gccttggcaa cgagctcctt gccggttcca ctctcgccct
      541 gaattagcac gtcgacagaa ctgtcgcgaa tttgatcgat caggcggacc actgattgaa
      601 tgcggtggct catgccgatg atgtttcggg ttgaaaactc gccaccgatt tcctttcctg
      661 catcaggatc ttgttggctt ggtagtaccc tgtcgatctg cgtggttttg gtggcgtcgc
      721 aaacatgcgc cgcgagtgat cgaagaattt ccgcgtcgtc ttcgttgaag attcccacct
      781 tcttgttgat tgcttcgccg acgccgatca cttcaccttt ggaattcatg attggaactg
      841 ccagcaacgt tttagtgcgg tagccgattt tttgatcgac ctctttgaag aacaacggat
      901 gttcataagc atccgtgacg ttgatcgtct gaccggtcat cgccgctgct ccggcaattc
      961 ccaagcgcgc atcgaacagc actatgcgtt tttctttttc atgagtaatg accgaccaga
     1021 gctcgcattt ctcgcgatcg agtaaaaaaa tgcttgcgcg atctgcttgg agcagctcgg
     1081 cggcttctgt agttatcagg tccaaaagag acatgatatt gcccaccgaa gtcattcgtt
     1141 tgcaggcgtt gagaacttcc ttcagcttct ccgagcgggg caccgacgct tcagaatcgg
     1201 gcggggcagg ctcgttcatt ggcttttgag taacacatca gcaaaaacgc tgtcaacgaa
     1261 gcctacatct gttaatcttg cgatggatgc tggttgtgct aggcactgaa cgcgcaccgt
     1321 tcgtcgcttg cagtttagtt tagccgcaca aaggcttttc ccgaatacca gccggggagg
     1381 cttcgatttg tcttgtcgat gaggcaagtt tgtgacccgc tgcacgtttc caagattcgg
     1441 taatcgagga gtttacatcg cattgtcgct gatcccaatt accgtcctcc cttgttcaac
     1501 gaagcttgga ataatttcca atgccaagct gcctctttcc gctttccagc tcgtgaatca
     1561 ttgttaatac ccttcggcac aggggcacag gaagattcat gctttcgcct tcgcgaacca
     1621 cataacccaa tgtgaagtcg acttcggtcg ggatgttttt ttgaatttgc cgcagcggtc
     1681 ctttgcggtc atcgtctttc catgattttg ccatctcatt gataatggcg agccttcttt
     1741 caatcggaat acttgggtga agatcaggcg gattgtattc tgcgagtcga ataaagcgtg
     1801 ctccggcgac agtccctact gcgaccactt ctcctaaaaa atccgcgagc aaccgccggt
     1861 tagcatcgcc ggcgcaactg gtcgccagcg cgaaatcggc gagggaccca aagaagccaa
     1921 ggcatgtata cgtgagcttg ctccagagca cgccgaggat gttgtcggag atctcagtgt
     1981 ctatcactgg atccagcaac ttgtgcaatc gactcagctg tggcgtggtc ctgccgtgca
     2041 aatggccgat atacagatgc ccgcgggtgg cggtttggac atgccccgga gcaaccctgc
     2101 ggcaacccat gcggatggcg atgcccacgg tccggtcggc gccgacttta tcctgaagca
     2161 ggggtggatt gataccgttt tgcatcgaca ccagcaggcc gtcatcgttg agggccggca
     2221 gcactgttgc aagcgcatca ggagtatagt tcgaacgcac ggcgataaag ccgatatcga
     2281 acttgccgtg gatctcttgc ggaaacacta tcttaacttt gacgttgaag gcgccgtccg
     2341 gcacgtccac ctgcaatccg ttggcacgga tcgcttcgac gtgttcttta tcgacatcaa
     2401 cgaaagtgac gtcgtggccg gcgcgcgcta ggcggccgcc gataatgccg ccgatgggtc
     2461 ccgttccgat aacgatcatg cgcatggagg agaagatcct ttcttaatac atgaaacgaa
     2521 cgattccagt cttatgcgaa atcgagcgac gcggtcaact cgtttctcct accagcggcg
     2581 gtcggtgatt atttttttct ttgtcttgac cctgctttca caagatccct atactttaaa
     2641 ctatgatccg cgccggagta ggacagtcta ctgaaccctc caccgagaaa gccgttcagc
     2701 aagcttttgc cgcagcgctt gcccaagcag agatcacgca agctgatatg gcactggtcg
     2761 tttttacggc ggaacacatg cgtgatcagg aaagacttcg caatgctgtg tctcatgcgt
     2821 ccaaccatgc cgtcgtggtc ggctccagcg ctgccggaat tctcaccgga gatggcgaga
     2881 ttgagggctc tcatgggatc gcggttcttg tgcttgcgtc ggatcagctg caagcgaagg
     2941 cctttctgtt tgaatcgctg cgcgggcatg atgcggaagc gggagttaaa atagcgcaac
     3001 tcgcccgacc cgttctggac agcaatcttc ttaccctgct tatgccggat agttacaacg
     3061 gccaagcaca agaaatgctg cacgcgatgc agcacgagat tggctactcg ccggtaatcg
     3121 gagcaggctc ttcggaaagc ggcgttgtcg gcgcgacatt tcagttgtgc aaggatcaag
     3181 ttgccagcaa tgccgttacc ggtctccatc tattaggctc ctttgaatca tcgatcgaca
     3241 tcacccaagg ctgccagcca attactgagc cgctggcgat caccagggcc gatggaaata
     3301 tcatctacga aatcgatcat cgtccagcat ttgaggtctt tacctctgtg ctcaaggggc
     3361 cgttgcttga agatctcagg cgagcattga tgtacgtgtt tgtcgggcta ccagccgatc
     3421 gcgggagaaa taccgtaggc ggaggggagt acctagtacg taacatcatc ggcttggatt
     3481 ccgagaaaaa aatcatcggc gtcgctgacc aagtgagtga agggcaagca atgatcttca
     3541 ccttacgcga cggccaaaga gcgcgcgatg atctggggca aatgctcaag cgtcaggctc
     3601 aacgcctggc gggcaaaaaa cctgctttcg gtgtttactt taattgttgc gcgcgcggcg
     3661 cgtcgctgta tggcatccct ggcattgaca gcgcgtacat tcgccagatc ctcggcgagt
     3721 ttcctctgat cggaatgttt ggcggatatg agcttgcgcc gctcggtggc gcgaaccatc
     3781 tttttgcata cacaggagtt ttggcattga tcgcagaagg caattaatcg gcgagttaga
     3841 aattagcacc gactgacgtt acgagattcg gctgcaaagc ctctggagaa ccgagcgagt
     3901 tacgcggctt gctagcggct gccgatccgc ttgcgcttat tcgctaaata gtcgacgacg
     3961 atgtaattgc ccatttgatc gggagaggta taaaaaaccc ggcccgcgtt gagtcgggag
     4021 agttggtcga caaacccctg gcggtagaaa tctgcgccca gcatgaaggt attgatcttg
     4081 atgccgctct gcgtgcagcg cctgacctct ttgagagttt cacgaacaat gcgaccgtgc
     4141 agcatcgctg agccccagcc gctgtgcaat ctgccaccct gatcgagagt cgccgcagtg
     4201 ggttcgccgt cggtgatgag aatgatctgg cggttcgccg atcggtttga accgagaagc
     4261 tgacgcgcga agcggagtgc ttccttgatg tcggtgccgt gctccatgaa ataagtcaac
     4321 gaaacaatcg acggcaaatc ttcggctctg atgattttcg catttgatcg gaatgccaca
     4381 aggtggagct tgtcttgcgg aaattttgtt tcgatcaacc ggtgcagggc gagggcgacc
     4441 tttttcccgg catgcaatgc atcgttcatg agcatcgaat agctgacgtc aatgcatagt
     4501 acggtttcgc tatcggtcga gaactcattg cgatgaactg aaaaatcttc cggccttatc
     4561 cgcaccggaa cgctgccgtt gccggtttcc agcgcgttca tcagggtctc ggagatattt
     4621 acattgagcg gatcaccaaa ctcgtaaggt tttgtttgct caagctgctc accttgggag
     4681 ccgcgcgccg aagtggagtg atcaccccaa cgattcttat tgagcatgtg aaagatttcc
     4741 cggagcgctt tgtcgccgat ttttctcatt cccttgggcg tgagtctcat gccgtgaccg
     4801 gtccttatga tgtacccttc ttcttcgagc atctgttcga cgcccttgag atacttaagc
     4861 tgctcgtagg cgtcctcgcc caacatattg cgtatctcat ccagatcgag ctccgacggg
     4921 tcgcccatgc cgcgctgccc ccagcggaga aatttttcca aaccgcgtag ttgaccgagg
     4981 atctggcctg cttcacccat ccccatccgt tgcgagcctt gaaacggata ctggttcatc
     5041 agctgctcaa gttgctgcat ctgattcatc atgtcactga gttgctgcaa ctgctcttga
     5101 gtgagcgaat cgaggttgtc cagcatattg tctagatcat cgaccgcggc atcgagcata
     5161 ttgggcagat tcttgagaaa attttcatcc tcaaggagct tgttcatccg gtccagcatg
     5221 ttcatcatgc cctgataatc ctgcccgccg ggattgctcg actgttggct gctgcgatct
     5281 gcgatctgat tctttaaacg ctgctgcaac tcttgatagc gttggtacgc atcctgcatt
     5341 ttacggttca tctcgtccat gctatccgcg tagcggccgg agagacgttc catgaagttc
     5401 cgcattttat cgtgataggt ttcgagcttt tcagccaggc tgttgagttt gtcgctcagt
     5461 tgttgggttt cttccggact caatttgaat gagtcaggag aatacttgtt gagcaattct
     5521 cgccgtttct catcggcttg gcgaagaaag tcacgcatac ctttgaccga gcgctgacca
     5581 tcttcggacg tcaggccgcg ccgcatcaga tcacgtaagg cccggcgcac cccttcctcc
     5641 tcgagaaggt tgtcagataa atgcttgagt aaatcttcgg tggtgggaaa gagaacctgt
     5701 tgtgtgccat cccaatatgt gtagcgatgg attctcataa tggcttgact atatcccagt
     5761 tcatgggtga aacaaaccct attttccgca gatggtttgt gtcgctggaa gccgcgaagg
     5821 acgagtacgc tatggccgaa tcaggccgaa tccgctatga atatcgttaa aggatctgaa
     5881 atatgatttc ctctgactgt gtaatcatcg gtgcgggctt tgccggcgct gccaccgctt
     5941 atcatctcgc ccgccgcggt ctagtcgata ttaccctgct ggaacaagaa gtgatcccgg
     6001 gctttcattc atcgggccgg aatgcggcga tgatccgtca atgcgtttct gaacctgctc
     6061 tctttgattt gactcgcgat ggcgccgctt tcttgcgcaa cttgcccgac gactggcccg
     6121 agccggtaca attcaaacag aatggctcac tcctgctcgc cagcgctgac ggctggaaaa
     6181 agctgcaaaa ggaagcggag ctcggccgca gcttaggagt agatatcgag ctgtggacac
     6241 ctagcgaggc gcggcggcac gtcgctgttt tagagaacgc gcagttcgac ggtgcggtat
     6301 ggtgcgctac cgacggggtc gtcgatattc atgggctgct ctcgggctat ttgaagtacg
     6361 ccaccgctca tgggttgcaa gttcactacg gtgctgacgt tcgcgccatt cgccgcaccg
     6421 ccgacggcgt cttcgaagtg cgcaccaaga agcaaatgct gaccacgcga atgatagtga
     6481 acgctaccgg cgcatgggct aatctcgtgg cggaaatggc cggagcgagc gcactgccgc
     6541 ttcgtccctg ccgccgccat ttatttacga cgccgccctt agcttggatc gattcgcatt
     6601 ggcctttcgt ctgggatgtc acacatgata tttattttcg ccctgagggc gaggggctgc
     6661 tactttgcgc ctgtgatcag caggaactat cgccgggaga cccgccgctg gatccgatga
     6721 ttggtgaggt gcttgcagaa aagattcagc ggtacatgcc ggccttagaa ggcgtgtcga
     6781 ttcacaaata ctgggctggc tttagaacaa tctcaagcga taatcgtttt gtcattggct
     6841 gggatccgaa cctcgaagga ttcttttggg tcgccgggct cggcggtcat ggagtaacta
     6901 ccagctcggc tgtcggtgcg ctcgcggccg atttgataat tgccggagat ggcaaaaagg
     6961 tcgaagcgtt ttcgcccagc aggttcaaga catgagtttg tgttttcctc gggcaagccg
     7021 atcgaaagtt ctcgttaatc tcgaagcatg ggagtagcgc agtcgtgaag ttgttttatg
     7081 cttccggctg cgtgagggca gcccggctga tagaattcaa agtctcgtat ccactcgcag
     7141 tcattcggtt agaccatttc gtctgacaac acacaccgaa gaaggagaag agaattgaaa
     7201 gatagtggct gggtcggaaa tactttgcat cgcggccata agaacgaatc cccttgacgc
     7261 gttggctttc cgcattatta gtctgtttgc tcactccttc gtccgctttt acgcagcggc
     7321 tccaactgct cgctatccca tttccgaagg ccgaatcgtc tcattggctt tggcaatttg
     7381 ccgtctctta tccaaccttc aatggttcgc tttgaaactc cgaaaagctg agcgacttgg
     7441 tctatgttta acaatttgcg catggtttga gctcacctgt gtgatgctat tgtaacagca
     7501 aacggctttg aatttgagcg tatataggac cgtgccagtt acatgcacaa tgtctttgct
     7561 catgggctcc aaataagtct taaacgaccc aacaaaattg gaatcgaaca gccttaatga
     7621 gtctggaggc ttactctgaa cggatcgcct cgtcgaccat cgccggtttt tttcaaatgc
     7681 ggattatgtt aaaaaattcg ttgtgacgcc ctcagatttg ctggcagcgt ggggaatggg
     7741 actgattatc gcaggggtgg cttttttcct gattcgcgat cttcggcgac ggtttgagca
     7801 aatcgacctc ttttttgatc gattggaagc ccggttttgg tatgtcccct ttatagctcc
     7861 ggcttccgcg ttattaggag tgtttctgat actgttggcg ttcgtgtttt aatctgcggc
     7921 ttcagagatt tttaacccgc tagatagaga caaccaactg ttgtggcggg tttctcgccg
     7981 cgttcaattt aggattcacg gcgttcgatg agaatcttgt tcccactatc atctaagaaa
     8041 aagcgctctc cttctctact ctgaaatacc gggcggccat cagctcgaat ctgagtgacc
     8101 cataccttca gttccggttc ggttcgcttt cgaggcgtct cagattgttt cggcgtcgtc
     8161 tgttttgcac tggtgatttc tggtttggga tgcgcttggg acgtttggcg gttggttggt
     8221 ttaaccattt ccttacgagc gatgcgctgt ccggactcac tcgattgcga acgattcccc
     8281 gggcgagctt gctttgcttc atcgaatgcg acaccctgcg gctcgagggt agtttctacg
     8341 atagcggctg gttgatccag cgtatttatc gcatctgcat acggccacgt ccgccccaac
     8401 gcgatgtact ggtttctcaa tctttcaatc atctgggttt ttgaatcaaa gctcagcctc
     8461 ttatagggcc cctcatgaca gtgataatac ttttgattct gatcgagatg acagccgaat
     8521 tgatcgagcc gcccgtcgtg agcctctgct ggcggggtcg acgccggagt ggcggccgcg
     8581 agggcgaaac ataaaacgat tagtttctta tccataagga agctcctgat gtctttgagc
     8641 atcgcgacaa gctgcatagt ttgttccagg gcagaagcta gtagtcaatt ggagttaaaa
     8701 gaggtttagg gcgagtcttt tgccaaagat gcgcaatgcc catggcgcta ctgacacagc
     8761 tgacatgaaa cttggatgct aagcgtccga caatggcgaa tgcttggaga gatttagatg
     8821 ggggcggagt tggcgtgacg gtatccacag cagcagccga atttattcgc gcaatagctc
     8881 ccgtagaaca tcgcacaagt tggttaatat ccgttgagcg aagggtcaga gtcacccgag
     8941 ctgccatctt cattgacaga ttgcgctcat atccgctagg gtgccgtgtc atatgaccaa
     9001 agacccgccg actcacatga gttctctgat gggacaatcg actcccgatg cgactttacg
     9061 ttcttctcct gacactgtgc tatggggtta tatcgcggcg aatctacccc cggcgctgag
     9121 cgtgaaatcc ggcgcaatcg tcgaggttga agcgctctcg caccaagggc taacgacgca
     9181 gaacgatcct gagaattttt tcgctaacta cggtatcccc ggcggggaag ttctgccgga
     9241 tgcaaaggcg gttttctccg gtgtagatcg tccgaaaggg gcgagcgttc atattctcac
     9301 cggaccgatc tacatcgatg gcgcggagcc gggcgatact ttggaagttc gcgttctcga
     9361 tatcaggttc cgcgtgcctt atggcgtgaa caataccggg cctggcaaag gtgtgttgcc
     9421 ccagctgtta aaagagcagt cggccagact gatccggctc gatttggagc ggcgagtcgc
     9481 gctcttctcc agcgatatcg aaatcccgct caatccgttc atggggatca tggccgtgtc
     9541 accgccaacc agccttggta tggtttcatc gacaccgccg ggcgcttggg gcggcaatat
     9601 ggatttgaaa tttctcggga ttggttcgtc cctctatctt ccggtgttca acaagggcgc
     9661 gcagttcttt accggtgacg gtcatgcggt tcaaggcgat ggggaggtcg atggcggcgc
     9721 aattgaaatc tctttggcgc cgaagctgca attcctgtta cacaaaggga agaggatcaa
     9781 gctgccgcgg gtcgaaacgg ccagtgatta tctattgacg ggtcttagca tcgatctaaa
     9841 tgaggcggcg cgcatagcgc ttcaggaaac cgtggagttt ctacaggccg aaaagaattt
     9901 aacggccgcc gatgcgtacg cgttgtcgag cttagccgtg gatctcggaa tcggcgaggc
     9961 agtggacatc gtcaacctgg tttatgccaa gatccctaag agaatcttca gaagcaatcc
    10021 accattctgg gcctgcacat aaccatagga gtccccagta ttctgcaccc gcgctccgtt
    10081 acatgccgaa ccgtggatct agcaatgcca tctcttgata agactcctac cggctatcag
    10141 taaaagttaa gtgcgagaat acctggacgt ggcttgaagc cgccgaccag ctgatcgcat
    10201 cccatctaga aagagcagca attattttct caagcatcgg taagatgcat ttgctgcttc
    10261 ccggcttgga taagcgaatt gtcaggattg gcgtccgcgg tctaatttga atattgtcct
    10321 gtattcgctc ttgaagtatc ggcatttcct tagggcagcc ttacgcgccg tatcgaaatg
    10381 aaatgctgct ctgcagtgca acaagcatga actcaggcgc aggcagattc gattggacgc
    10441 ttcaaactga cattgcagtg tgccaagctt gcgttgcaag taaactgtcg aactcagcta
    10501 aaacaaggag taaggcgatc ctgcttactg cgtcaggaaa atcttgcgga agtcttcctt
    10561 cagtttgcta aactgttcgt ctgacgggtt ctccatgaac actgcatgag caaccagctt
    10621 ctcggcgtct ttaagattgg gataaatccc cggagcgatt gggaagtcgc cgctatcggc
    10681 gataatcttt tgcacctccg ccgtgcacag ataatcgatg tagagcttgg ccgcattggg
    10741 agcagccgct ttggcactga gagcgagatc ggtcgaatcg gtcaaaactt tgtcgaaaac
    10801 gacatggctt agcggaccct tcacctgagt aatatactgc gcataagtaa ttccaagttg
    10861 cgcttcacct tttactaagg cggtcggcac cggtgcgaac gattccagca gaaacggctt
    10921 ctgtttagcg agcgctctgg cataatccag ccacttctca cctttaactt tctgcatgtt
    10981 ccacagaaac tgcgctgtgc tcgtgtgcct ggaaggatcg ggcatggcga ttttgctttg
    11041 ccattttgga ttcaatagat catcgaagtc ttttggcaga tcgccgggcc tcaccagttc
    11101 tgtgttatag aggatgctta tcggggtgtg gcgaaaggcg gtcagcaccc catccttgtc
    11161 tttcaagtgg gcgggaaact tggcggccgc gggtgggatg tacttggcta gggcattttc
    11221 gcgtttaagt agagttatcg ttccattgat tgcaaataaa acgtcgaacc ctggcttgcc
    11281 ggcgcgccac tcggtgattg ctcgatcggt aacggcggtg gctgaggcgc gccaataatc
    11341 aactttaact ccgtagcgtt tttcaaaccc agcatgaata ggcgccatga cattgggaat
    11401 cgttgtgcca tagacaacga tcctgccttc ctttttggcc gtctcgagat caagcgtctg
    11461 agccaaaagg ctattgctga aacacagaac catcaagcta atcgatagaa ccagctttag
    11521 tttcatttca atcgattcaa acattttagc tattctccgc tgtgacgtta atgcttattg
    11581 atctcactaa cggcggtcgg cgtatcattt cattgtcata ctccgagcga ccgcgcaccg
    11641 tcgatggcgc atacaagata cattcgtccg tgtgacgtga tcaagcgtag tcgatcgccc
    11701 tcgattcatt cttcggctca cttatccgga aaatgcgtaa tcgaacgcat gattaaaggg
    11761 ctttatcttc ttgttgctgc ttgagcacgg aagccagaat agagatcagc aagatcccgc
    11821 cgaccacagc taacgaaatg ccgatgggga ttttgtaaaa ctcggcgatc agcattttcg
    11881 cgccaacgaa acagagcaca aacgcgagac caaccctcag ataacgaaac atttccatga
    11941 cgccggcaag gaggaagaaa agagcccgaa gtccaagaat cgcaaaaaca ttggatgtat
    12001 agacgataaa tggatctaaa gtgatcgcga agatcgccgg gatcgaatcg acggcgaaaa
    12061 taacatcggt ggtctcgacc acgacaagca ccagaaacag cggggtagcc cacagctttt
    12121 gatccttgcg gacgaagaat cgctgtccct ggtaatccgg ggttacgttg acaaaacgac
    12181 ggaccaatcg gacagcggga tttttttctg gctcaacctt ttcgtcgccg acgaatagca
    12241 actttattcc ggtgaagacg agaaagccgc caaagacgta aatcatccag tgaaagttgg
    12301 acagcaaggc ggtgccggtc gcaataaaac cggctcgcaa gaccagagcc cccagaatac
    12361 cccagaagag gacccggtgc tgatagctgg acggaacgga gaaaaacgag aaaattacca
    12421 ggaagacaaa aagattgtct acactcaagg accactcgat tacatagccg gccaaaaact
    12481 ccagcgcctt ctcgccacca cgaaagaaat tgatgccgac agcaaaaata agcgctaagg
    12541 aaacccagac acaactccaa accagagcct ctcgcaggga aacagcatgt gctttgcggt
    12601 gaagtactaa aagatcaagg gccaggacgc ctaaaacaaa aacgttaaaa acgatccaca
    12661 agaagagctg cgaatccatc cgttgtatat cccttcagct tcaataattg gcgctttaaa
    12721 cttgcagaac cacgttcaag gcgcgtagag tttgcgcgga gatgagcgcc ggacggcgaa
    12781 ttgtgcgata aggcaagccg aatgcggcgc aaattctgct ggccgtcacc gtttgcgcgg
    12841 ctggtaggaa tttgcggcta ttggcgcgaa atcgtcgaat cgtggaagaa gttgtcgaag
    12901 cgttcgtccg atccgaactg acagactgat ccgagataat atgcgtacaa tcttcgcgtt
    12961 gatacgaatc tatcgctaag ccggcggtga gaaaaaaaaa tagcaggatc caggaagata
    13021 gcgccggttt catcagaggc agggctatca attcataggc atttagtcaa tcaagcttgc
    13081 agccgaagat gaaccccgcc ggttgaagag cggcgcgggc gatcaaccgc tggccatcag
    13141 tgaattcgcc ggctaatggt ggctctgtct ctgcatacat gagaggcttc gtttgatcat
    13201 aaacgaccca ggagaaggtt cgcctataag cctaagcgct ggctgacgct ttctgaatct
    13261 aagcctcgtc gccgcttaga tcggctttca cgggttcttg cacatgcggc ggcttaggat
    13321 ctttcggctt gatcagccaa gatgcgataa tcgagagcaa caacaccgag ccgatgatcc
    13381 ccaaggaaac cccgattgga atcttataga tgtcgacgag catcattttg gctccgacga
    13441 agcaaagcac aaccgataga ccgactttca ggtacacgaa catttccatc acaccggcca
    13501 gcataaagta gagggcgcgc agcccgagaa tcgcaaagac attcgatgtg tagatgatga
    13561 aagggtcatt cgtgatggcg aaaatcgcgg gaatggagtc gacggcgaaa atgacatccg
    13621 tagtctcgac cacgatgagc accagcatta agagcgtggc gtaagtctta ccttcacgtc
    13681 gaacaaaaaa tttctgacca tcataatttc tgatcacggg cacaaatcgc tcgaacagcc
    13741 tcaccacggg atttctttcc ggtgcgattt tttcgtcgcc ctgaaggtac atcttgattc
    13801 cggtgacgac caggaagccg ccgaaaacgt aaatcatcca gtggaaattg cttaacagcg
    13861 cagaccctgt ggcgatgaac accgcgcgta atactaacgc gccgaggatg ccccaaaaaa
    13921 gcactcggtg ctggtactgg ctgggcgtat tgaaatattg aaagatcatt agaaacacaa
    13981 acaagttgtc gacgctgaga gatttttcga tgacataacc ggtgaggaac tccagggctg
    14041 cttcggatcc ccaggcataa taaatgccaa cattgaagat catcgccaag cagacccata
    14101 cgacgctcca aatcgatgct tccttgatcg agatcgtgtg agccttgcgg tgaaaaacac
    14161 ccaaatccag ggcgagcatc cccaggacaa aaaggttaaa gccaatccaa agaaataatc
    14221 gttccgattc catatccgtc actttccgtt c
//
LOCUS       DASUVS010000405        21030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1928556, whole genome shotgun sequence.
ACCESSION   DASUVS010000405 DASUVS010000000
VERSION     DASUVS010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 21030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21030
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928556"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..982
                     /gene="larB"
                     /locus_tag="VF182_21680"
     CDS             233..982
                     /gene="larB"
                     /locus_tag="VF182_21680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEX6439742.1"
                     /translation="MDSERLVQLLEEVREGRTSIGHAVARLRHMPYEDLGFAKVDHHR
                     AIRQGFPEVILGQGKDPKEIAAIIGALRRQKTNILVTRVSPEKMTSLKKTVAGLKYHA
                     TARAVTWIPKPIAIKGKGVVLIVCAGTSDIPVAEEALLTARLMGNRVEKLFDVGVAGI
                     HRLLENRPRLDQAAVVIVVAGMEGALPSVVAGIIDKPVIAVPTSIGYGASFNGLAALL
                     GMLNSCAAGVTVVNIDNGFGAGFAASLINRV"
     gene            1173..1598
                     /locus_tag="VF182_21685"
     CDS             1173..1598
                     /locus_tag="VF182_21685"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEX6439743.1"
                     /translation="MDNIKRYRITPHHSELAVKVFGSSQLELFQNSGWALFDVTTDME
                     KIESKDCVPLEVEGTDRDDLLVNWVRELLYLYQGSGYLLKEFRISELGDKLVKAEACG
                     EKIDPDRHEIKKEVVAIAYDKSRMVKTGDQWTAQLIFEV"
     gene            1605..3008
                     /locus_tag="VF182_21690"
     CDS             1605..3008
                     /locus_tag="VF182_21690"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX6439744.1"
                     /translation="MSYVTESDYEGIALIADPIHRYIQFTVPQSKGEVTEKALIDSTW
                     VQRLRYIYQLQSARWVYPSAEHSRFQHSLGAMHMAGEFAKHLYPSLRKVLGDECPSAP
                     FIEEYMRVTGLLHDLGHGPFGHFFDDNFLHDFGVTHEDLGQAIIQKYLADIIGQLRRS
                     PSGPFAAGEELRPEDVAFPIKKNAQEDRDKPNWVRMLQPLTSGIFTFDNLDYVSRDSY
                     MCGIAGGAVDRARLMHYTFFSPKGLTLHKAGNSALTMFLNSRLYLYTNVYYHRTTRAI
                     DLHLRDIFPETMRVIFPGNPLEHLEEYLYLTDWSLLEGVSRWQRKKGAMEALANEWQT
                     VLHREVKWKMAYDRTLSLNELHYGMALIQVSDWEKRIRSALPADMSSLRFRIDMATQD
                     PRPENPFAMGKKQIHIFDPSTGEITKESLAELFEFIPSKVVQFRVFALDHEHDQVLAE
                     IAENTLKGQSVSTKTSV"
     gene            3328..3906
                     /locus_tag="VF182_21695"
     CDS             3328..3906
                     /locus_tag="VF182_21695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HEX6439745.1"
                     /translation="MTKAIIKHFEESIRVKQSFLRDNVETLAQAIDTVVKAFQSGHKL
                     LLFGNGGSAADAQHIAAEFVNRFLIERPPLPAIAFTTDTSVLTSIANDYSYNEVFAKQ
                     VQALGKDGDVAIAISTSGNSSNVIAAIDACKKLNITTIGLTGGKGGQMVGRVDFLLNV
                     SEGRNSARIQETHILIGHVICDLVDQKLFPPE"
     gene            3919..4866
                     /locus_tag="VF182_21700"
     CDS             3919..4866
                     /locus_tag="VF182_21700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439746.1"
                     /translation="MPKKLFKPLDFSKVKTYPAKKRLSKVQTAALGKVIQRGSRVRSF
                     VEGLPDILAAQSLKAIAKKIAQTHTVNRTVLLGMGAHPIKIGLSPLIIDFMRKGIIDA
                     VALNGAGIIHDFELAFMGETSEDVEASLKDGSFGMAEETGAFINQAIIDGSTQGLGIG
                     AAVGQAIVQARLKHQELSILATAARIGIPVTAHIAIGTDIIHMHPKADGKAIGEGSMR
                     DFRTLTCVVASLANGVYLNFGSAVILPEVFLKAVSLARNLGNPVQKLTTINLDFLAQY
                     RPMTNVVQRPTSASGKGYHLTGHMEIMVPLLFAAVLENL"
     gene            5101..5871
                     /locus_tag="VF182_21705"
     CDS             5101..5871
                     /locus_tag="VF182_21705"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX6439747.1"
                     /translation="MTDFGHADPFVGIMKGAILSITSNIQIVDLNHGIPPQDIRAAAL
                     TLAASFNYFPQGSIHVAVVDPGVGSKRLPIVIHAAGHYFVGPDNGIFGLALKDQESSF
                     IVHLSNEAYHLQPTSQTFHGRDVFAPVAAHLARGVPMTEFGSPLEDCVRLAWPAVERA
                     GKFILGEVIYIDRFGNLFTNIRERDLEGEQRDRIRIYLKQIKIQGLSQSYVAGNKGKY
                     IALINSWGLVEVASYNDSAASRSSSRIGNKVRLELLAV"
     gene            5895..6350
                     /locus_tag="VF182_21710"
     CDS             5895..6350
                     /locus_tag="VF182_21710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HEX6439748.1"
                     /translation="MSVRLSWEQYFMTITRQVAERSTCTRAKVGAVIVRDKSILATGY
                     NGAPAGMPHCTDVGCLIYESVTPNGETEQNCFRTIHAEMNAIAQAAKNGSTIKDASIY
                     ITHTPCIHCLKVLVNTGIKDIFYEKPYKLHTLEELLSYTQVDLHKIDLP"
     gene            complement(6398..6730)
                     /locus_tag="VF182_21715"
     CDS             complement(6398..6730)
                     /locus_tag="VF182_21715"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX6439749.1"
                     /translation="MKKVWSVLSAVFVTLMTSSLALAAAEGVAVTGADSQVKAAIAIA
                     AGLAVAVAAFGAALGQGRVGATAMESIGRNPNAADRLFLPLLLSLALLEALALYGFVI
                     AFLLQGKI"
     gene            complement(6821..7609)
                     /gene="atpB"
                     /locus_tag="VF182_21720"
     CDS             complement(6821..7609)
                     /gene="atpB"
                     /locus_tag="VF182_21720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX6439750.1"
                     /translation="MEHPFTWYVLLPHNWQAHLQQHTFFALVSSLFVLLFAWRGRAAL
                     ARAQDPAVPAAELGTRNIAELLVGLVVSQSDAVIGRQGRKFVPYFGTFFLFILFSNLS
                     GLLPGFSPPTSDLNTTVALALVSFIGYNLIGIKEMGTGYLKHFVGPMTGLPAQSFLGK
                     LAFVPLLVLSVSFFFVLEFVSHLFRPVSLALRLFGNMMGDHIVIEVFMDLTKVIVPVI
                     FYALGTLVSIIQAAVFMLLSMIYVSLAIAHGHDEAHGNEQEHVH"
     gene            complement(7619..7990)
                     /locus_tag="VF182_21725"
     CDS             complement(7619..7990)
                     /locus_tag="VF182_21725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439751.1"
                     /translation="MKQPAGTATIVRIELWHGLLLLATALTLGQSRFIELKALLLGGV
                     FMGVNFFLLGFGILWVLPPLASKGRVTAGVSLLILKIIFFLSLLTLLFFRFHIDGLSF
                     ALGVSTLILAIFVEAVIYSFK"
     gene            complement(8223..9515)
                     /gene="hemL"
                     /locus_tag="VF182_21730"
     CDS             complement(8223..9515)
                     /gene="hemL"
                     /locus_tag="VF182_21730"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX6439752.1"
                     /translation="MRTAKSKRLFESAQEKIPGAVNSPVRAWQAVTGVPRFIARGKGA
                     HIVDVDDNEYIDYVGSWGPLILGHAPPYVTAALRDALRQGTTFGAPTERELRLAETVC
                     RLMPSIEKIRLVSSGTEATMSAIRLARAFTKRNKIVKFDGCYHGHSDGLLVKAGSGVT
                     TLGLPDSPGVVPALAAETLVARYNDFADIERIFSTYGNEIAAVIVEPVCANIGVIPPS
                     GKFLPDLASIIRRHGSLLIFDEVVTGFRLAPGGAQELYRLHPDLTCLGKILGGGLPLA
                     AFGGRRDIMNLVAPLGPVYQAGTLSGNPLAVTAGLATLKQLSRRGTYQRLEKNSKYLA
                     EGFAKVIAKYKTKATVNRVGSMMTLFFGVERVSNADEARHCDREKFARFFHGMLERGI
                     YLPPAPFEAMMISLSHSTVDLRKTVAAFDSWSERDGHC"
     gene            9892..11247
                     /gene="dnaA"
                     /locus_tag="VF182_21735"
     CDS             9892..11247
                     /gene="dnaA"
                     /locus_tag="VF182_21735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX6439753.1"
                     /translation="MLAMWEEALRQLREKVGRQNFETWIKPIHVNSITAESIVLAVPN
                     KFFRDWLSEHYYSQISEVLESLTHKDLKISLTINSQSATARPEKAPRSDNNDKKEEHE
                     LHPKQHSRTNNLVPKYTFENFVVGASNQFAHAASMAVANQPGDHYNPLFIYGGVGLGK
                     THLANAIGHRAAATRQGLKIFYLSSESFMNELIGSLRRDKMDEFKTKFRNIDILIVDD
                     VQFIAGKERTQEEFFHTFNSLYESHKQIVITSDKFPKEIPGLEDRLRNRFEWGLIADI
                     QPPDIETRVAIIQKKAEGEGIHLPHDVAIFLATNIESNVRELEGSLTRLGAFSSLTKS
                     VITVDLAKEVLHNTLKGAKKEITVESIQKTICDFYNLKLGDLKAKRRTKNIALPRQVA
                     MYLCRKYTETSFPAIGDKFGGRDHSTVIHASKTIEKRIKEDPFMQNTIERLERNLNIN
                     K"
     gene            11475..12581
                     /gene="dnaN"
                     /locus_tag="VF182_21740"
     CDS             11475..12581
                     /gene="dnaN"
                     /locus_tag="VF182_21740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX6439754.1"
                     /translation="MEIKAKRGDLLATLYWTQSIVERRNTMPILANVLLESQKKDIRV
                     TATDLEVGVRGSLEGDVVKEGSVTVNAKKLYEIVREVPNEHIQLKRLENDWVEIRSGK
                     SVFKIVGMDAREFPQFPKFSRDGLTTTPASTLREMIEGTIFSVSTDETRYSLNGVYVE
                     QGEAGNVRLVATDGHRLAFDERNLGSFGLAKGVILPRKGLAELKKLLESGDDGVVSIG
                     FKENMGLVMKDRVELFMRLIDGDFPDYTKVIPKGNQNIARIEHDEFLQALRRVSIVSS
                     ERYKGIKMEFNEDKLSVSANNPDLGEATEEVEMEYKGNPISIGFNARYLIDVLHVLGS
                     DGEVQIELKDALSPSLVKKGGKEGYLYVLMPMRL"
     gene            complement(12625..13488)
                     /locus_tag="VF182_21745"
     CDS             complement(12625..13488)
                     /locus_tag="VF182_21745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HEX6439755.1"
                     /translation="MNKAELIRRSLKTTGQLIMPGVYDALSAQIATRAGFEVIFISGY
                     SLAATMLGEPDFGILTQTEMLSAAQRICSVTDLPVIVDADTGYGNAINVIRTVNELIR
                     IGAGGMFLEDQVWPKRCGHMKGKQVIPLEEQLKKLQAAIEAKKNRDLFIVARTDARQA
                     LGLNEAIERGIAFKQVGADAVFIEAPESKEELLEIARCVPGPLVANMLERGVTPLMGP
                     QELRDLGFDLVVWPLAPLYSVAKSLNEVYTTLRRKGTTAEILERLMPFDEFNSIVGLE
                     EKYQLDEKYRS"
     gene            complement(13558..13923)
                     /locus_tag="VF182_21750"
     CDS             complement(13558..13923)
                     /locus_tag="VF182_21750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439756.1"
                     /translation="MFGKGVHQEEEALLREIIRTIDKKMDYSAREGEGSRFTLHFTAR
                     GHEAVVSLDLQDLKAALRDMTRRNQIRQKIKARYDHLDQSRYGADILGLKSAKLLRAA
                     PKPEPGRQQRGFGRSGPRR"
     gene            complement(14367..15332)
                     /locus_tag="VF182_21755"
     CDS             complement(14367..15332)
                     /locus_tag="VF182_21755"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439757.1"
                     /translation="MSKKVHLTLACGDYESIRALKEGTVKPDGIDLTVLTDMTSDIRH
                     WRMVRNREFDIAELSMSNYIMAKYVGLPFTAIPVFLHRRFRHSFIFLNTGKGITKPAD
                     LIGKKVGLRNFQATANLWIRGILEHEHKVPHRQIHWFKQDDEEVEWTPPADLHLERIA
                     SGKQVEQMLVDGELDALVHPELIKPILEKDPRVNRLFPNYRDLEMDYFRRTGIFPIMH
                     TTAIKQEVADKYPWAPINLMQAFEESKNAAYKRLENPRIVPLAWFRHFLEEQEDIIGK
                     DPWVYGLGDTNRKTLETLMQYSQEQGMLGRKMMLEELFINTAAHS"
     gene            15444..16412
                     /locus_tag="VF182_21760"
     CDS             15444..16412
                     /locus_tag="VF182_21760"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439758.1"
                     /translation="MKRCYTSLFLLLLSLSLAIPVLAQDPVRVNWTAVTGAQSGMFMA
                     QQEGLFKKSGVNIELIHIPSSSRGIQAILAGEISFSFMDGVNAAQANLKGANLVLVAG
                     ATNRQVFSLMGKPEYKRIADLKGKKIGITRVGSSTHTSALFALASAGLRTNDYQLLPL
                     LEVPNIYTALAAGQIDAGIMSPPTNARAKRAGFVELMNVAKEGPEFVSVAVGTNRSYI
                     KSNEENVRRVVRAYAEGVQLFKNNKPAAIRMIQKQLKVTDPEIQEDTYNQFREYLEYP
                     PYVSRKGLEAVLGELADKESAAKNIKPEDLMDMRFVAELDKKTGGR"
     gene            complement(16477..17181)
                     /locus_tag="VF182_21765"
     CDS             complement(16477..17181)
                     /locus_tag="VF182_21765"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6439759.1"
                     /translation="MSNTKKLKENLALSCNILAYEGHWDNILGHVSVRIPGQQRILMK
                     PHSFGFEEIRPEHIIEVDLDGNKVAGKYERHSEVFIHTEIFKARSDVHCVVHSHPPHA
                     VALGSLGQPLRPISHEGNIFYEGLPLFDLTTALIRTSELGVEVAKSLGKCRGLLMKNH
                     GSTVVGESIEVATLYAIFLEKAARIQLMATASGDPSWTSDEESAIKYDQIYTPHRLGS
                     MWDYFVRRAKKFRKSA"
     gene            complement(17226..18767)
                     /locus_tag="VF182_21770"
     CDS             complement(17226..18767)
                     /locus_tag="VF182_21770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017437729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX6439760.1"
                     /translation="MGVDLPEIYNAATTFVDENIAQGRGGRIAIYYQDEAITYQEVYE
                     KVNRTGNALKELGVEIEHRVLLALPDSPEFAYSFFGAIKIGAVPIPTNPWMAAKDYEY
                     LINDSRARAIIVHESVLPEISKIWDNTRYLKQILVVGKGKERARSYQELIDRASDYLE
                     AEPTHRDDVGFWGYTSGSTGMPKGAVHLQHDMITITDLFVKPVLGMNENDRCFSASKL
                     FFSYGLGNSLYFPFRFGASTVLWPEKPDPEKILQVIDKYKPTFFFSVPTLYARFLRIE
                     KKYDLSSLRLCLSSGEPLPPAIFQHWQERFGMELLDVVGSTEATHDFLANRPGRAKAG
                     SSGEVTPAFEAKIVDDAGREVPLGEIGNLMVKGDANSPCYWNKHEQTKRVMQGEWLKT
                     GDTYYSDAEGYYWYCGRSDDMMKVGGLWVSPIEIENTLMEHPAVLESGVIGDTDADGL
                     LKPKAYVLLKPEIKPSDELRLELQTHVKTRLAPYKYPRWVEFVEDLPKTVTGKIQRFR
                     LRAGR"
     gene            complement(18830..20302)
                     /locus_tag="VF182_21775"
     CDS             complement(18830..20302)
                     /locus_tag="VF182_21775"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018461143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6439761.1"
                     /translation="MAKLLIASEHVDSESHETTAVKNPATGELVDSVPKGTVNDIRRA
                     IDAAKGALKKWSELAPSKRGAILLQAGHAILQQEKDLATLLTREQGKPMRESILEIRR
                     FVHTLDHYGGMAKTLRNAAVMLDAGRHGLVLRKPLGVCGSIVPWNFPVSLMGNKLGPA
                     LLTGNTVVVKPAGTTPLTDLRCCELIDKAIQDAGGPRGVLNVVTGPGSVVGEELLVNP
                     TVRKIGFTGATDTGRRVMQSAAKDFKHVTLELGGSDPMIVCDDADIDRAVSAASVGRF
                     FNCGQACLAVKRLYLFDRIADNFMDKLVEKVKKIGVGNGLRSGVLMGPLHTAEQRREV
                     EEQVEDAVKRGAKVLYGAQRPKGDDYEKGFYLQPTLVADVDPASRLLQEECFGPALPI
                     VRVKNLDHAIEQANNSIFGLGSSIWTRDINRAMAGAERIEAGYTWVNSAQIIYDELPF
                     GGVKQSGLGKEHGEEAIEHYTDSKSVVIATETHSEMVGGE"
     gene            20891..>21030
                     /locus_tag="VF182_21780"
     CDS             20891..>21030
                     /locus_tag="VF182_21780"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HEX6439762.1"
                     /translation="MTQQKEVAVFGGGCFWCNEAVFDELWGGTSIVSGYAGGNTKNPT
                     YE"
BASE COUNT         5361 a         5286 c         5325 g         5058 t
ORIGIN      
        1 cgctcgcccg agtgatcgac agctaattga ccgattgcag catcaggtta gagtgccgct
       61 aacccgcgtc ggctcctgcg tcccggctaa tcaaggagtt ttttgtttgg actgttcagg
      121 caaacccatt tcagttccag cggtcggtca tgatcatttc aagaggaaaa cttccgaccg
      181 ccatgattaa tttcgtcaac tcggtcgccc gaggctctgc cggatgtgat ggatggactc
      241 tgaacgtctc gtccagttat tagaagaggt gcgcgagggg agaactagca ttgggcacgc
      301 tgtcgctcga ctccgtcata tgccctacga agatctgggc tttgccaagg ttgaccacca
      361 cagggcgatc cgacaaggtt ttcccgaagt aattttaggg caggggaaag acccgaaaga
      421 gattgccgcc attataggtg cgctccggcg ccagaaaacc aatatactgg taactcgggt
      481 gtccccggaa aaaatgacaa gtctaaaaaa aaccgtcgcc ggactcaagt atcatgcgac
      541 cgcgcgggca gtgacctgga taccgaaacc gattgctatc aagggcaaag gagttgtgct
      601 gatagtgtgc gccggcacct ctgatattcc tgtcgcagaa gaggctttgt taaccgcaag
      661 gctgatgggc aaccgtgtcg agaagctgtt tgacgtcggc gtggctggca tccatcgact
      721 tctcgagaac cgaccgagat tggaccaggc ggctgtggtc attgttgttg cgggcatgga
      781 aggcgcgttg ccaagcgtcg ttgccggaat aatcgataaa ccggttatag ctgttcccac
      841 cagcatcggt tacggagcaa gcttcaacgg acttgcggct ttattgggaa tgcttaactc
      901 ttgtgccgca ggggttacgg tagtcaatat agataatggc tttggcgcgg ggtttgcggc
      961 gagtcttata aaccgggtat agattacctc gtggcggtaa acggtgagaa tgcatgaaca
     1021 ctttgctttt gtcggcagcc tgtttcaata cgttcatcga actacaaaat attgcttcac
     1081 ggatcactag tacttcgcca accgaactca ctaacgctct tgtcagtatt gtttaaacgc
     1141 actacgtcag ttctctttaa ggccgcagga tcatggataa tataaagcga tatcgcatta
     1201 ctcctcatca ttcagaattg gccgtcaaag ttttcggctc ctcgcaattg gaattattcc
     1261 agaattcggg atgggcgcta tttgatgtta caaccgacat ggaaaagatt gaaagcaaag
     1321 attgtgttcc ccttgaggtg gaaggaaccg accgggatga tttattggtg aactgggtgc
     1381 gtgagctcct ctacctctat caaggcagcg gctacttact gaaagaattt cggatcagcg
     1441 agctcgggga taaactagtg aaagcggaag catgcggcga gaaaatcgat cccgatcgcc
     1501 atgagattaa aaaggaagta gtggcaattg catatgacaa gagtcgtatg gtgaaaaccg
     1561 gcgatcaatg gaccgcgcag cttatctttg aggtctaatc attaatgagt tacgtcaccg
     1621 aaagcgatta cgaaggaatc gcgctcattg ccgatcccat tcatcgctac atacagttca
     1681 cagtgccgca gagcaagggc gaggtgacgg aaaaggcttt aatcgattct acttgggtgc
     1741 aacgcctccg ttatatctat cagctccaaa gcgcacgctg ggtttacccc tccgcagagc
     1801 atagccggtt tcagcattcc ttgggcgcca tgcacatggc cggcgaattc gctaaacacc
     1861 tatatccgtc attacggaag gtcctaggtg atgaatgtcc gtcggcccca ttcatcgaag
     1921 agtatatgcg ggtaacgggc ttgcttcatg atctcggaca tgggccgttt ggacatttct
     1981 tcgatgataa ttttctgcac gatttcggcg ttacccacga ggatctaggc caggctatca
     2041 ttcaaaagta cctggcagat atcatcgggc aactacggcg cagccctagc ggtccatttg
     2101 ccgccggtga agagctgcgt ccggaagatg tcgcttttcc aatcaagaaa aatgctcaag
     2161 aagatcgtga taagcccaat tgggtgagaa tgttacaacc attgaccagc ggtattttca
     2221 catttgataa tctcgattat gtttcgcgcg actcctacat gtgcggcatc gccggtggag
     2281 ccgtcgatcg tgcccgtctg atgcactaca cttttttttc gcctaaaggt ttgacgctgc
     2341 acaaagccgg caacagcgct ctaaccatgt tcctcaactc tcgactttat ctttacacca
     2401 acgtgtatta ccaccgcacc acacgtgcca tcgatctcca cttgcgtgat atctttccgg
     2461 agacgatgcg ggtaattttt ccgggaaatc cgctcgaaca cttggaagaa tacctgtact
     2521 tgaccgactg gtccctgctc gagggcgtct ctcggtggca gcgcaaaaaa ggagcgatgg
     2581 aggcattagc taacgaatgg caaactgtgc tgcaccgtga ggttaaatgg aagatggctt
     2641 atgatcgaac tctttctctt aatgagcttc attacgggat ggcactgata caggtctccg
     2701 attgggagaa gcgaatccgc agcgctctac ccgcagatat gagtagtctc agatttcgca
     2761 tagatatggc gacccaggat ccgcggcctg agaatccgtt tgccatgggt aagaaacaga
     2821 ttcacatctt cgatccttcc accggtgaga tcacaaaaga atcgttagcc gaacttttcg
     2881 aatttatccc gtccaaggtt gttcaattcc gtgtctttgc cttagaccac gagcacgacc
     2941 aggtgcttgc cgaaatcgca gaaaacacct taaagggaca atcagtgtcc accaaaacga
     3001 gtgtctagga agatattcgg taactccgtc tctagcaaag cagccgttca ctgggccacg
     3061 taagctgata ggtgataatt tacgagaggc ccaccttcgc ccgcgccgct ctgcacaatt
     3121 gtgaaggtat ttacacttca aacgaaagtt ctttatcaat cggacgaatc tgagatgcaa
     3181 ttattggctc tcccccctcc aaagtctgcc gagccagagc ctgaacaaaa agctgtatac
     3241 cccaactgac acagctcatg tagctttacg cctccacgag cgtgtgttag ataaagcaaa
     3301 cggcgtctta gaggtagcaa aaaagcgatg acaaaagcta ttatcaagca tttcgaagag
     3361 agcatacggg taaagcagag cttcctccgc gataacgtag aaactctcgc gcaggcaatt
     3421 gataccgtcg tcaaggcctt tcaaagcgga cataagctgc tcctcttcgg taacggcggt
     3481 agcgccgccg atgctcagca tatcgccgct gagttcgtaa atcgatttct gatcgaacga
     3541 ccgccgctac cggcaatcgc tttcactacc gatacgtcag tgttgacaag catcgctaat
     3601 gattacagct acaatgaagt gtttgcgaag caagtacagg cactgggtaa agacggtgat
     3661 gtggctatcg caatatcaac cagcggtaac tcctcgaacg taattgctgc gattgatgcc
     3721 tgcaaaaaat taaacattac gacaatcggc ctaaccggcg gcaaaggagg gcagatggtg
     3781 ggaagggtag actttctact caacgtttcc gagggccgca acagtgcgcg tattcaggaa
     3841 acacatattc tcatcggtca tgtaatttgt gatctggtgg atcagaaact ctttccccca
     3901 gagtaaaccg tgccttccat gccgaagaag cttttcaaac ctctagactt ttccaaagtt
     3961 aagacctatc cggcgaaaaa acgactcagc aaggtgcaaa cggcagctct tggaaaagta
     4021 atacaaagag gcagtcgggt gcgctcgttc gttgaaggtt tacccgatat cctcgcagcc
     4081 caaagcttga aggctattgc aaaaaaaatc gcccaaactc acaccgtcaa tcgtaccgtg
     4141 ctgctcggta tgggcgccca tccgattaag attggactaa gcccgctgat catcgacttc
     4201 atgaggaaag gtatcatcga tgcggtggca ctgaacggcg ctggaattat tcatgatttt
     4261 gaactcgctt ttatgggtga aacctcggaa gatgtcgagg ccagtctaaa agacggaagc
     4321 tttggcatgg ccgaggaaac cggtgcgttt atcaaccagg cgattatcga tggttcgaca
     4381 cagggtctgg gcatcggcgc tgcggtggga caagcaatcg tccaagcgcg tttaaaacat
     4441 caggagctga gcatacttgc cactgccgcg cgaatcggaa ttcccgtcac ggcacacatc
     4501 gccattggca cggatatcat tcacatgcat ccgaaagccg atggaaaagc tattggtgaa
     4561 ggaagcatgc gagacttccg caccttgacg tgcgtagtcg ccagcctcgc caacggcgtc
     4621 tatcttaact tcggctctgc tgtaatactg ccggaggtat ttctaaaagc cgtaagcctg
     4681 gcccgcaatt tgggcaatcc cgtgcagaaa ttaaccacga tcaatctaga ttttctcgct
     4741 cagtaccgtc ctatgactaa cgttgtccag cgccctacat cggcaagcgg caaaggctac
     4801 cacctaaccg gtcatatgga gattatggta ccgctgttgt ttgcggcagt gctggaaaat
     4861 ctatagctgc ggatctccgt aggatgattt cagtacttgc ctcgcacttg ttcttgattt
     4921 aggcaaaata tcatccccag cagcagaacc ctcaatcgcc tcgtagagat agttccgttg
     4981 aaccttcgaa caaagactac cggcgatcgg gttagcagtc acggtgattg ctttacccga
     5041 tctctgtttt ctgcttagat ttatcctgcc gtgaattccc agacaccgat catcacgttg
     5101 atgaccgatt tcggtcatgc ggatcctttt gtcggaatca tgaaaggcgc aattctaagt
     5161 atcacttcca atatccagat cgtcgattta aatcacggga ttccgccgca ggatatcagg
     5221 gcggcagcgt taacattagc tgcatcgttt aactattttc cgcaaggcag cattcacgtg
     5281 gccgtggtcg accccggcgt cggcagtaaa cggctaccga ttgttattca cgcagcgggg
     5341 cactactttg ttggtccaga taacggtatt tttggtcttg cactcaaaga ccaggaatca
     5401 tctttcattg tgcacttgtc gaacgaagcc tatcatctgc aacccacaag ccagaccttc
     5461 cacggcagag atgtatttgc gccagtggcg gctcatcttg cgcgtggagt tccgatgact
     5521 gagtttggct cgccattaga agactgcgtg cgcttagctt ggccggcagt agagcgagcc
     5581 ggaaaattca tccttgggga agtaatttat atcgatcgct ttggcaatct gtttacaaac
     5641 attcgcgagc gcgatctgga gggagagcag agagacagga ttcgcatcta tctgaaacag
     5701 attaaaattc aagggctatc gcaaagctac gttgcgggca ataaaggcaa atacatcgcg
     5761 ctgatcaata gctggggatt ggtcgaagtc gcgagttata acgatagcgc ggcaagccgc
     5821 agttcgtcca ggattggcaa taaggtgcga cttgaactac ttgctgtttg aaagcaaatc
     5881 acccggagga gaccatgagc gtgcggctta gttgggagca gtattttatg accatcacgc
     5941 gacaagtggc ggagcgatcc acttgcacgc gggcgaaggt cggcgcggtc atcgtgcgcg
     6001 acaagagcat tctagccacg ggttacaacg gcgcgccggc tggcatgcct cactgcactg
     6061 acgtcggctg cctgatctat gaatcggtaa cgcccaacgg cgaaaccgaa caaaactgct
     6121 ttcgtacgat tcatgcagaa atgaacgcga ttgctcaagc ggccaaaaat gggtcaacca
     6181 tcaaggacgc ttcgatctat ataactcata cgccgtgcat tcactgcctt aaggtgctgg
     6241 tgaacacggg cattaaagat attttttacg aaaagccata caagcttcat acactcgaag
     6301 agttgctcag ctacacccaa gtcgatttgc acaagatcga tctcccataa aaaaggcgcg
     6361 gaaacaatcc gcgcctcttt ttagtttctc gccgcagtta tattttgccc tgcagaagaa
     6421 acgcgataac aaacccgtac aacgccaacg cttcaagaag cgccaagctt aacagcaatg
     6481 gcagaaacaa tcgatcggca gcgttagggt ttcggccgat gctttccatc gctgtagcgc
     6541 ctacccggcc ctgacccaag gcagcgccaa acgcagcaac ggcaaccgcc aagccggcag
     6601 caatggcaat tgcagctttg acttgcgagt ccgcccctgt cactgcgaca ccttcggccg
     6661 ccgctagagc caagctcgat gtcatcagcg tcacgaacac tgcgctcaaa accgaccata
     6721 cctttttcat cttgcctcct ttccagcgag ttggactaaa acctcctctt cctctatctc
     6781 tccaccgtcg tccttcgctt tgattacctg agagttgatc ctagtgaacg tgctcctgtt
     6841 cattgccatg agcctcatca tgtccatgag caatagccaa gctgacataa atcatgctta
     6901 acagcatgaa cacggcagct tgaatgatcg aaacgagcgt accaagagcg taaaaaatca
     6961 ctggcacaat gactttcgtg aggtccatga aaacctcgat gacgatatga tcgcccatca
     7021 tgttgccgaa aagccggaga gcgagggaaa ccggccgaaa taggtgagat acaaattcca
     7081 aaacgaaaaa gaaagacacc gagagtacca acagaggcac aaacgcgagt ttgccgagga
     7141 agctctgcgc cggaagacct gtcatcggcc cgacaaaatg ctttaagtac ccggttccca
     7201 tttctttgat gccgatcagg ttatagccga tgaacgaaac cagtgccaaa gcaacagtgg
     7261 tgtttaagtc acttgtaggc ggtgagaacc cgggcagaag tccgctcaag ttactgaata
     7321 gaataaacag aaagaaggta ccgaaatagg gcacgaattt gcgtccttgc cggccgatca
     7381 ccgcatcgct ttgactcacc actaagccga ccaggagttc ggcgatgttt cttgttccca
     7441 actctgcggc gggaacagcc ggatcctgtg cacgtgctag agccgcgcgg ccacgccagg
     7501 cgaaaagcag gacgaataaa gagctcacta aagcaaaaaa ggtgtgctgc tgaagatgcg
     7561 cctgccagtt atgcggcagg agcacatacc aagtaaaggg atgttccatt atttgatcct
     7621 acttgaacga ataaatcacc gcctccacga aaattgccag tatcaaagtt gaaactccga
     7681 gcgcaaagga caatccatcg atgtggaatc ggaaaaacaa gagcgtcaaa agactcaaaa
     7741 aaaaaatgat tttcaagatg agcagagaaa cgcctgctgt caccctgccc ttgctcgcca
     7801 ggggcggaag cacccagaga atgccaaacc caagcaaaaa aaaattcacg cccataaaga
     7861 caccgccaag gagaagcgcc tttagttcaa taaatctgct ttggcccaga gttaacgcgg
     7921 tcgcaagaag caataaccca tgccaaagtt caatgcgaac aatggtggct gtaccggccg
     7981 gctgtttcat tcgttggctt gaaacggagc cttattacgt ccagctgagg gggtcatgta
     8041 cttcggcgat gcagaagtta gtatcgccat aacggggcat cccttcaaaa tagccagaaa
     8101 acctcgcaca actgcaacac acaactgctt ttggcttagg aacggtgttc gaatcccttc
     8161 ggctcagagg tttcgcgctg cagcgaccgg cggtagagtc ttaacatgaa ggtattgggg
     8221 ggtcaacaat ggccgtcacg ttcggaccag gaatcaaagg cagcaacggt cttacgtaaa
     8281 tccaccgtcg aatgagacag ggaaatcatc attgcttcaa acggcgcagg tggcaagtag
     8341 attccccgct ctaacatgcc atgaaaaaac ctagcgaatt tttcgcgatc acagtgtctc
     8401 gcctcatcgg cattggatac acgttcaact ccgaagaata aggtcatcat tgaaccgacc
     8461 cgattgacag tcgccttggt tttgtatttt gcgatcactt tggcgaaacc ttccgccagg
     8521 tatttgctat ttttttccag cctctgataa gtgccgcgac gcgagagctg tttcaaagtt
     8581 gccaggccgg ccgtgaccgc gagtggatta ccggacaagg tcccggcttg ataaaccgga
     8641 cctaggggcg cgaccaggtt cattatatcg cggcggccgc cgaacgccgc gaggggcaaa
     8701 ccgccgccca gaatcttgcc caagcaggtc agatctggat gcagccggta gagttcttga
     8761 gcaccgccgg gtgcaagacg aaagcccgtt actacttcgt caaaaataag caagctgcca
     8821 tgtcggcgaa taatacttgc gagatccgga agaaactttc cggacggcgg aatcacgcca
     8881 atattggcgc aaacaggttc aacaattact gccgcgattt cattgccgta tgtggaaaaa
     8941 attctctcga tatccgcgaa atcattataa cgtgctacga gcgtttcagc agctaacgcc
     9001 gggaccactc ccggactatc cggcaatccg agtgtggtta ctcctgagcc tgcctttacc
     9061 agcagcccat ccgagtgacc gtggtaacaa ccatcaaatt ttacgatttt gttgcgcttg
     9121 gtgaaggcac gcgctaaacg tatcgcgctc atcgtcgctt cagttccaga actcaccaga
     9181 cgtatttttt ctatgctcgg catcaaccga cagacagtct cggccaatcg cagttctcgc
     9241 tccgtcggtg ctccgaatgt tgtgccttgc cgcaacgcat cgcgtaaggc agccgtaaca
     9301 taaggcggcg catggccaag gatcagcggt ccccaagagc ctacgtaatc gatgtactca
     9361 ttgtcatcga cgtcaacgat atgcgcacct ttaccacgcg caatgaaacg cggcacgcct
     9421 gtcacagcct gccatgctcg tacgggactg ttcacggcgc cgggaatttt ctcttgggcg
     9481 ctttcaaaaa gtcgctttga tttggcggtt ctcattagtg cagttcagct agcatcggct
     9541 tatgagtgtg tcaaggttgc tgaaacgata agaaaaaaaa tttagaaaat ttttccttga
     9601 aatggattat gaaaaggatt atgacttgcc cttcggcaaa atttttgtag ttgttctcgc
     9661 ttcgctcagg cgtaaggttg cgggttttca ttgctcgttt ttggagcgaa cagaatagca
     9721 agaatatgcc agtcattctc gctccgtgca tttgtcgatc gaggtcgctg tcaattccgg
     9781 taactagttg aaagccaagt tgttttttaa ttatccacag ctgtggataa cgttgttcgt
     9841 aactacaata atttttcttt taaaaattag ctacttagtt ggtgccgaac tatgcttgca
     9901 atgtgggaag aagctctcag acaattgaga gaaaaagttg gaagacaaaa ttttgaaacg
     9961 tggatcaaac ctattcacgt caattccatc acggccgaaa gtattgttct tgccgttccg
    10021 aacaaatttt ttcgggattg gctaagcgag cattactatt cgcaaattag tgaagtcttg
    10081 gaatctttga ctcataagga tttgaagatt tctctcacta tcaatagtca atcggctacc
    10141 gcgcgaccgg aaaaagctcc tcgaagtgat aacaatgata agaaggaaga acacgagctt
    10201 catcccaagc agcattcgcg cactaacaat ttggttccaa aatatacttt tgaaaatttt
    10261 gtcgtcggcg ccagcaatca attcgctcat gcggcctcga tggcagtagc caaccagcct
    10321 ggcgatcact acaatccttt gttcatttat ggaggggttg gcttgggcaa aacccacctt
    10381 gctaatgcga tcgggcatcg cgcggctgcc actcgtcagg ggctaaagat tttttatctc
    10441 agttcagagt cctttatgaa cgaactaatc ggttcgctta gacgggacaa aatggatgag
    10501 ttcaagacta aattcagaaa tatcgatatt ctaatcgtcg atgatgtcca gttcatcgcc
    10561 ggcaaagaga gaacgcaaga agaatttttc catactttca actcgcttta tgaatcgcat
    10621 aagcagatcg ttatcacctc tgataaattt ccaaaggaaa ttcccggtct cgaagatcgg
    10681 ctgcgaaacc gctttgaatg ggggctaatc gcggatatcc aacccccgga tatagagacg
    10741 cgagtggcga tcattcaaaa aaaggcggaa ggagagggca ttcacctgcc tcatgacgtg
    10801 gcaattttct tggcgacaaa tatcgaatcc aatgtcagag aattagaagg ttccttaacc
    10861 cgtcttgggg cgttctcgtc cctgacgaaa tcggtaatta cggtcgatct cgctaaggag
    10921 gtgcttcaca ataccctcaa aggcgcaaag aaggagataa ccgtcgagag cattcaaaag
    10981 acaatatgcg acttttacaa cctcaagcta ggggatttaa aggccaaaag aagaacaaaa
    11041 aatatcgcat taccaaggca agtggcgatg tatctttgtc gaaagtatac cgaaacttcc
    11101 tttccagcca ttggagataa gttcggcggc cgcgatcact cgaccgtgat tcatgcgtcc
    11161 aagacaatcg aaaagaggat caaagaagat cccttcatgc agaacacgat tgagcgatta
    11221 gagagaaatc taaacatcaa caaatagctg tggataagct gtgacgggtc gccagaacaa
    11281 ttgtggacaa ccggggataa ggacggcatg cgccgtcgtg acccgcttag cgcgtttaac
    11341 gttgcggctt catacacagg ccaagctcca gtagttgctt ctcttttagc gattatcccg
    11401 gttgtgaaca gtctctattg ctactacttt actgatttat atatataaga gaaagaagag
    11461 agtcgggagg aatcatggaa attaaagcaa agcgaggaga tcttctcgcc acgctttatt
    11521 ggactcaaag catcgttgaa cgccgcaata cgatgccgat cttggcgaac gtcctcctcg
    11581 agtctcagaa aaaagatatt cgcgttaccg ccacagacct ggaagtcggt gtaagaggaa
    11641 gtctcgaagg agatgttgtc aaagaaggct cggtcacagt caatgcgaag aaactttatg
    11701 agatcgttcg cgaggttccg aacgaacaca ttcaacttaa aagactggaa aatgattggg
    11761 tagaaatccg cagtggaaag tcggttttta aaattgtcgg aatggatgcc cgcgagtttc
    11821 cgcaatttcc aaaatttagt cgcgacggtt tgacgaccac acccgcttcc acgcttcgcg
    11881 aaatgattga gggcacaatc ttttccgttt ctacggacga aacgcggtat agtttgaacg
    11941 gagtttatgt tgagcagggc gaggccggta acgttcgctt agtagccacc gatggtcacc
    12001 ggctggcttt tgacgaaaga aacctcggct ccttcgggtt ggcaaagggg gtaattttgc
    12061 ccaggaaggg gttagcggaa cttaaaaaac ttttggaaag cggcgacgat ggcgttgtct
    12121 ccattggctt taaggagaac atgggtcttg taatgaaaga cagagtcgag ttgttcatgc
    12181 ggctcatcga tggtgacttt ccagattaca cgaaagtgat acccaaagga aatcagaaca
    12241 ttgctcgtat cgagcacgat gaatttttgc aagctctgcg ccgggtctca atcgtttcaa
    12301 gcgaacgtta caagggcatc aaaatggaat tcaacgagga taagctttct gtttcagcaa
    12361 acaatccgga tcttggcgaa gccaccgaag aagtagagat ggagtataaa ggaaacccga
    12421 tcagtatagg cttcaacgcg cgctacttga ttgatgtgct ccatgtgctc ggctctgatg
    12481 gagaagttca aatcgaattg aaggacgcac ttagcccgag cttggtgaag aagggaggca
    12541 aagaaggcta cctttatgtc ctgatgccga tgcgattgta ataaggcaac tcggtgggac
    12601 ttgcgatatt aattgttgcg tcaactagct ccgatatttt tcgtccaact ggtatttttc
    12661 ttctaaaccc acaatactgt taaactcgtc aaagggcatc aagcgctcca atatctccgc
    12721 ggttgttcct ttgcgacgca gcgtcgtata aacctcgttc agcgacttcg ccaccgaata
    12781 aagcggcgcc aggggccaaa cgaccagatc gaatcccaga tcacgcaact cctgtggtcc
    12841 catcaaagga gtgacaccgc gctcgagcat attggcgact aaagggccgg ggacacagcg
    12901 cgctatctcg agcaattctt ctttactttc tggagcctcg ataaacaccg catctgcgcc
    12961 gacctgcttg aatgcaatcc cacgctcgat cgcctcattc aaccctaggg cctgccgcgc
    13021 atcggtacgc gcaactataa ataagtcgcg gttctttttt gcttcgatgg cagcctgcaa
    13081 tttctttaat tgctcttcga gtggaatgac ctgcttgcct ttcatgtgcc cacatcgttt
    13141 aggccatacc tgatcttcta gaaacatgcc gccagcgccg attctaatca attcattgac
    13201 agttctaata acgttgatgg cgttgccgta gccggtatcg gcatcgacga ttaccggcaa
    13261 atcggtcacg gaacaaatgc gctgagctgc cgacaacatt tccgtttggg tcagaatgcc
    13321 gaagtcaggc tcgccgagca tggttgcggc aaggctataa ccgctaataa agatgacttc
    13381 gaaacctgcc ctagtcgcga tttgtgcgct cagcgcatcg tacaccccgg gcatgatcaa
    13441 ctggccggtt gttttcaatg atcgacgaat caattccgct ttgttcatgg gaactcctaa
    13501 gcagatgagg cagccgatca ggcaagtgct tcgttgaacg gacgttaaga ggtgaactca
    13561 gcgccgcggg ccgctgcggc caaagccacg ctgctgcctt ccaggctcag gtttcggggc
    13621 ggctcgcaat aatttggcgg atttcagacc tagaatgtcg gcgccatagc gtgattgatc
    13681 caggtgatca taccgagctt ttatcttctg acgaatctga ttgcgccggg tcatatctcg
    13741 cagagcagct ttgagatcct gcagatcgag cgagaccaca gcctcatgac cacgcgcagt
    13801 aaaatgcaga gtgaatcggg atccttcccc ttcgcgggcg ctgtaatcca ttttcttatc
    13861 gattgtacgg atgatttccc ggagcaaagc ttcttcttct tgatgaacgc cttttccaaa
    13921 catgatgtcc ctctctggca aatagattgt gccctgagct acttaccgga tgagaaagtg
    13981 agcacccatc tgccaagtac cgccaggttg taaaaattta cgccaaagcg caggtaagat
    14041 caatcaccga ccgagaatgt ctgttcaaag atttcaaaat gattcaatac tagctcgcgc
    14101 gtcgagtttg tgcgttcgcg aggatcgggg ggtttgcttg agggtgaaaa ggaccatatc
    14161 ggcagtgcta tcgggtgagg agtgcccgtc ggatgaaagg gtagtacggg cggtctggtt
    14221 tgggtctcga gcgcgaagtt tgcgccgtgt cgattgttcg tcccaatcac atgggacaac
    14281 gtggattccg ttcgggtgaa atttcccgcg agataaagcg ctacagctta tccttggcgc
    14341 gcaacggcgc gcagtttcca taacaactaa gaatgggcgg cggtattgat gaataactcc
    14401 tcaagcatca ttttacggcc gagcatgcct tgctcttgag aatattgcat caatgtttcc
    14461 agggtcttgc gattggtgtc gccaagtccg taaacccacg gatctttgcc gataatatct
    14521 tcttgttctt ccaaaaaatg acgaaaccag gctaatggaa cgatcctggg attttccagt
    14581 cgcttatacg cagcgttttt agattcttca aacgcctgca tcaggttgat cggcgcccac
    14641 ggatacttat cggccacttc ttgcttgatt gcggtggtat gcatgatcgg gaaaatgccg
    14701 gtgcgcctaa agtaatccat ttccaggtca cggtaatttg gaaaaagacg gttaactcgc
    14761 ggatcttttt ccaaaatcgg tttaatcagc tcaggatgaa ccaaagcgtc taattcgcca
    14821 tcgacaagca tttgttcaac ctgttttccc gaagcgatcc gttctaagtg caaatccgcg
    14881 ggcggggtcc attccacttc ctcgtcatct tgtttgaacc agtgaatctg tcgatgcggg
    14941 accttgtgct catgctccaa aataccgcga atccataaat ttgccgtggc ttgaaaatta
    15001 cgcaggccca ctttcttgcc gattaaatcg gctggtttgg tgatcccttt gcccgtattc
    15061 agaaagataa agctatgccg gaagcgacgg tgaagaaaaa ccggtatcgc agtaaacggc
    15121 aggccaacat acttggccat gatataattc gacatcgaca gttcagcgat atcgaactcg
    15181 cgattgcgaa ccatccgcca gtggcggata tccgaggtca tgtcggtcaa aaccgtcaga
    15241 tcgattccgt ccggcttaac ggttccttcc ttgagcgcgc ggatgctctc atagtcgccg
    15301 caagccaacg tgaggtgcac tttcttactc atgcggccta tctagcacgg tttattgcga
    15361 ttgccaacga attgcgaatt gactctcttc taggtctttg gtattacgca tgggccgaca
    15421 acatgagatc ggagtttccc tagatgaaga gatgttatac gagtttgttt ttgctacttc
    15481 tcagtttatc cctggcaatt ccggtgctgg cgcaggaccc cgtgcgcgtc aattggaccg
    15541 cggttaccgg cgctcaaagc ggcatgttca tggcgcagca ggaaggatta ttcaaaaaaa
    15601 gcggcgttaa catcgaattg attcatattc cttccagctc gcgcggcatt caggcgatac
    15661 tggcaggcga aatatctttc tccttcatgg atggcgtcaa tgcggcgcag gccaatttaa
    15721 aaggggcaaa cttggtcttg gttgctggag caaccaaccg gcaagtattt tcgctcatgg
    15781 gcaaaccgga atataagcgc atcgctgatt tgaaaggaaa gaagatcggc atcacgcgag
    15841 tgggttcctc gacgcatact tcggcacttt tcgcgcttgc ttccgcgggc ctgagaacta
    15901 acgattatca gttgctgccg ctgcttgagg tgccgaatat ttacaccgcc cttgcagccg
    15961 gccaaatcga cgctggaatc atgtcaccac cgaccaatgc gcgagcgaag cgggccggat
    16021 tcgttgagct gatgaatgtc gccaaagaag gtcccgagtt tgtttccgtc gcggttggca
    16081 ctaaccgttc gtatatcaag agtaatgaag agaacgtgcg ccgggtggtt cgagcgtatg
    16141 ccgaaggcgt tcagctattc aaaaacaaca agcctgcggc gataagaatg atccaaaagc
    16201 agctcaaggt aaccgatcct gaaatccagg aagacactta caatcaattc cgcgaatatc
    16261 tcgaatatcc accttacgtc agccgcaagg gtttggaggc ggttctcggt gaactggctg
    16321 ataaggaatc agctgcaaag aacataaagc ccgaagacct gatggatatg cgctttgtcg
    16381 ccgaactgga caagaaaacc ggcggaagat agcggtttta agcgatccag cggttaagcc
    16441 gagcatgaac actgattctt atagccagct taggtcttac gcggatttac gaaacttctt
    16501 ggcgcggcgg acaaagtaat cccacattga accgagacga tgcggcgtat agatctgatc
    16561 gtatttgatc gccgattctt cgtcgctggt ccatgacgga tcacctgaag ctgttgccat
    16621 aagttggatg cgcgctgctt tctccaagaa tatggcatag agcgtggcca cttcgatact
    16681 ttcgccgacc acggttgatc cgtgattttt cattaatagg ccgcgacact tgccgagact
    16741 cttggctacc tccactccca attccgaggt tcggatcagg gcggttgtga gatcgaatag
    16801 cggcaaacct tcataaaata tgttgccttc atgactgatc ggacgaagcg gttgacctag
    16861 cgacccaagc gccactgcgt gtggtggatg cgagtgcaca acacaatgaa cgtctgatct
    16921 cgccttaaaa atttcagtgt gtataaatac ctccgaatga cgctcatact tgccagccac
    16981 tttgttgccg tccaagtcga cttcaataat atgttcgggt cggatttcct caaagccgaa
    17041 gctgtgcggt ttcatgagaa tgcgctgttg gccgggaatt ctgactgaca catggccaag
    17101 gatattgtcc cagtgaccct cgtacgcgag aatgttgcag gataacgcta agttttcttt
    17161 gagttttttc gtgttgctca tagcagtgct cccttactta atgatttgaa gttaatgatt
    17221 gaagcctacc ggccggcgcg caggcggaag cgctggattt tgccggtgac ggttttagga
    17281 aggtcttcga cgaattcaac ccagcgcggg tatttgtaag gcgcaagcct ggttttgacg
    17341 tgggtctgga gttctaaacg cagttcatcg ctaggtttaa tttcgggttt caagaggaca
    17401 tacgctttcg gcttgagcaa gccgtcagcg tcggtatcgc cgatcacacc cgactcgagc
    17461 acagccggat gctccattag ggtgttttca atttcgatgg gcgataccca taagccgccc
    17521 actttcatca tgtcgtcgga gcgaccgcag taccagtagt aaccctcggc atcgctatag
    17581 tacgtatctc cggtttttag ccactcgccc tgcataacgc gcttggtctg ctcatgtttg
    17641 ttccagtagc acggagagtt ggcgtcgcct tttaccatta agttgccaat ttcccctagc
    17701 ggaacttcgc gcccggcatc gtcgacgatt ttggcttcga aagccggcgt gacttcgccg
    17761 ctgctgccgg ccttagccct gcccggacga ttggcgagaa aatcatgagt agcctccgta
    17821 gaaccgacga catctaacaa ttccatgccg aaccgctctt gccaatgttg gaagattgcc
    17881 ggcggcaacg gctcgccgga tgaaagacat aggcgcaacg agctcaagtc gtatttcttc
    17941 tcgattcgta agaatcgcgc atatagcgta ggcacggaaa agaagaaggt cggtttatac
    18001 ttgtcgatga cttgaagaat tttttctgga tccggttttt cgggccagag aacggtggag
    18061 gcgccgaatc gaaagggaaa gtacagtgag ttgcccaatc cataagagaa aaagagcttc
    18121 gatgccgaaa aacagcgatc gttttcattc atgcccagaa ccggtttcac gaacagatcc
    18181 gtgattgtaa tcatgtcatg ctgcaggtga actgcacctt tgggcattcc agtactgcct
    18241 gacgtgtaac cccaaaaccc cacatcatcg cggtgggttg gttctgcttc caagtaatcc
    18301 gatgctcgat cgatgagctc ttgataagat cgcgcccttt ccttgccttt tcccaccaca
    18361 agaatctgtt tgaggtatcg cgtgttgtcc cagatcttgg aaatctccgg gagcacggat
    18421 tcgtgcacga tgatggcgcg cgctctactg tcattaatta ggtattcgta atcttttgcc
    18481 gccatccagg gattcgtcgg aatcggcacg gcaccgattt tgatcgcccc aaaaaagcta
    18541 taagcaaact caggggagtc gggaagggca agtaataccc gatgctcgat ctcaacaccg
    18601 agctctttta aagcgttgcc ggttcggttt actttctcgt agacttcttg gtaggtgatc
    18661 gcttcgtctt ggtagtagat ggcgatcctg ccgccgcggc cttgggcgat gttttcatcc
    18721 acaaaagtcg ttgcggcatt atagatctca ggaagatcga ctcccatccc tcgctccggt
    18781 cggaattcaa gctatctctg aacgcggcac cttggattgc cgtaggactt cattccccac
    18841 cgaccatttc cgaatgtgtc tcggtagcaa tgaccaccga ctttgagtcg gtgtaatgct
    18901 cgatcgcctc ttcaccgtgt tccttgccca gaccgctctg tttgactccg ccgaagggta
    18961 actcgtcgta gatgatttgc gccgaattga cccaggtgta accggcttcg atgcgttccg
    19021 cgccggccat cgcgcgattg atgtcgcgcg tccaaatgga tgagcccaac ccaaagatcg
    19081 aattgttggc ttgctcgatc gcgtggtcga gatttttaac ccgaacgatc ggcaaggctg
    19141 gaccgaagca ttcttcctgc agcagccggg atgccgggtc gacatcggcg accaaagtgg
    19201 gctgcagata aaatcctttc tcgtagtcat cgcctttcgg tcgttgcgct ccgtagagca
    19261 ctttggcacc gcgctttacc gcgtcttcaa cttgttcttc aacttcccgg cgctgttcgg
    19321 ctgtatgcag cggccccatg agcacgcctg acctcaagcc gttgccaacg ccgatctttt
    19381 taactttttc gaccagcttg tccataaagt tatcggcgat tcggtcgaac agataaagcc
    19441 gtttgaccgc cagacaggcc tggccacagt taaagaagcg gccgaccgaa gcggcgctga
    19501 ccgcgcgatc gatgtcagca tcgtcgcaga cgatcatcgg atcgctgcca cccaattcca
    19561 aggtgacatg cttgaagtct ttggcggccg attgcatgac tcggcgaccg gtatcggtgg
    19621 ctccggtaaa gccgatcttg cgcactgtcg gattgacgag taattcttcg ccgacaacgc
    19681 tcccgggacc ggtgacgaca ttgagaacac cgcgagggcc gccggcatct tgaatggctt
    19741 tatcgatcaa ttcgcagcat cgcaagtccg tcagcggggt cgtgccagcc ggtttgacaa
    19801 caacggtatt gccggtcaac agggcggggc cgagtttatt gcccatcaaa gacacaggaa
    19861 agttccaggg cacgatggag ccgcagacgc ccaaaggctt acgcaaaacc aaaccgtgac
    19921 gaccggcgtc aagcataacc gcagcgttac gcagagtctt tgccatccca ccgtaatgat
    19981 cgagagtgtg cacaaagcgg cgaatctcca aaatggattc ccgcatcggt ttgccttgct
    20041 cccgcgtcaa caaagtcgca aggtcttttt cctgttgcag gatcgcgtgg cccgcttgaa
    20101 gtaaaatcgc gccgcgctta gacggcgcca actcggacca ttttttcagc gcacctttgg
    20161 cggcatcgat ggcgcggcgg atatcattca cagtgccctt aggaacggag tcgacaagtt
    20221 ctccggtggc tggatttttc actgcagtgg tctcgtgact ttcggagtcc acatgctcac
    20281 tggcgataag cagttttgcc ataattcctc cgtaacttag aaatccaaaa acaatcatgt
    20341 agctaagtgg ttgtcaagat tgcgggagtt tcgagatggg aatcagaatt cgacagtttg
    20401 gagccgtgta ctgcgcggcg ccaggattct taaatttcga tagcggactt atgcttgata
    20461 ggcaggaaag gaactcatga ctgggctgac atgaaactcg gcggaattga ttgatttgat
    20521 cgggtgccat tgatagaact ccctccaatg aaacgttcct atctgatcaa cgatggttaa
    20581 accgtgctga tgatttctct cattagcgtt aggaggaatt gcacgcgcag cgctcgcagt
    20641 accaagaatg atggtaactt tcggcacggc ccagaagagg tttaccgcac tcagtttata
    20701 ccagtcatcg catcactgcc gtagtacatt ctaaaacgaa cgtggcagcg gaattgccga
    20761 attgccggtt cagaacggta tggggcgtta agcccgaagg agcaacgcag tgcgtgaagc
    20821 ttatcggttc gcttgcttgc gaaagtgagt cggccgctac atacgatagt agcaagggag
    20881 ttggggcacg atgacacagc aaaaggaagt cgctgttttc ggcggcggat gtttctggtg
    20941 caatgaagcg gtatttgacg agctctgggg cgggacttca attgtctccg gctatgctgg
    21001 cggaaacacg aaaaatccga cttacgagca
//
LOCUS       DASUVS010000406         7661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1275403, whole genome shotgun sequence.
ACCESSION   DASUVS010000406 DASUVS010000000
VERSION     DASUVS010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7661
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275403"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VF182_21785"
     CDS             complement(<1..810)
                     /locus_tag="VF182_21785"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX6439763.1"
                     /translation="MKLYKKSRLLTLLGLCFLSLALAGQLSAQEKSFLWKVDSDRNSL
                     YILGSIHLLKKEAYPLKQSIENAFEQTNKLVLEIDLRSANPERIQQLMLQKSASTDGA
                     FLQRNVSDETYESVARRANELGLDIRLLNSFKPWIVATTMAAVKLEKLGFDPKLGVDR
                     YFAERAIQSNKPTLGLETAEFQLDLFDRFSPKDQELLLRQSMNEMDRLEQNVAQIVRA
                     WKSGDVGSLEKHLLAGMRDYPEIHRKVIDDRNRRWLPQIEGLISRGENALII"
     gene            complement(1010..1333)
                     /locus_tag="VF182_21790"
     CDS             complement(1010..1333)
                     /locus_tag="VF182_21790"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX6439764.1"
                     /translation="MGVSYIPIARIGELAPGVMKEVDLGGRQVLLTFVEGKYFAFSKQ
                     CPHEEADLQSAGQLIDGTKLRCNNHGYCFDLKTGECLLPQGGPPLTVLPAEEKGEDVC
                     IRLEW"
     gene            complement(1338..3035)
                     /locus_tag="VF182_21795"
     CDS             complement(1338..3035)
                     /locus_tag="VF182_21795"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6439765.1"
                     /translation="MAYDLLIRGGRVVDGSGLPSYVADVGVKGGKIVEVGRLNGTAAR
                     TIDAGGLAVSPGFIDHHTHLDAQMLWDPYGTCEPQHGVTSVVMGNCGLSLAPVKKGDE
                     DAIVKSFVRVEAIPRFALEQGVKWGWSSYGEYLDALEGRLGINVGGLVGHIAMRHNVM
                     GEEAVERTASAKEVQQMRALVLEAMEGGALGLSTNRNERHMREDGKPVASRLADDDEL
                     FALCDVLAEHNGGVIETILGRNKIEHFKFYHELAKRTQRPVLWQSLQHRWAEPTLWRE
                     QLDAVEPIFREGYQAYGLSHTVPLVRHFELRNCQVFDEFPTWKNLMFLPVEVRKQAFA
                     DGQTRQKLRADLANPAPTNFHRNWNIVRVEKVVKPENEKYNGKTIAELGAMRNQDPLD
                     AFLDLALEEDLGTVFWNANNGGDVDAMGEILRSPYVLIGTSDAGAHVQFGADFGYCTI
                     LLGMWVRERQVMSLEQAVHKLTFHVASIFGLQDRGLLRPGYAADLAIFDPKTVNSHEP
                     EWAHDYPAGSKRLIQRSVGMHYTVVNGTVIHEDSRMSGDLPGQVLRGPLYREQKVAA"
     gene            complement(3217..3615)
                     /locus_tag="VF182_21800"
     CDS             complement(3217..3615)
                     /locus_tag="VF182_21800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX6439766.1"
                     /translation="MEELVQHTGACHCGRVRFEIDAVSIIDALECNCSICSKGGFLHL
                     IVPRSRFRLLQGSADLTTYTFNTGVAKHLFCKTCGIKSFYIPRSNPDGYSVNVRCLDT
                     TNIRQVNVSSFDGKNWEGHGGELAHRSLEP"
     gene            complement(3648..5930)
                     /locus_tag="VF182_21805"
     CDS             complement(3648..5930)
                     /locus_tag="VF182_21805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HEX6439767.1"
                     /translation="MSLIHSTLSERRLVIVSNRLPFNVKVENGKITFHESAGGLVTGL
                     ASFMQSRRRTPALSMEHLWVGWPGSTVEVSLQGQLIREALSSFQSYPVFLSEEQMEKF
                     YLGFCNSTIWPLFHYFPTFTEYRPNYWEEYKQINQNFCDALDDVVRPNDILWIHDYHL
                     MLLPRLIKARRPQASVGFFLHIPFPSFEVFRLLPGEWRREILDGLLGADLIGFHTYEY
                     THHFLQCVLRILGYEHQMAQLLTPDHLVKIDTFPMGIDFDKYSNAPAEPDTQQEIQAL
                     RSTLSEFKMILSVDRLDYSKGILHRLEGYELFLENNPQFHGKVVLLMIVVPSRIGVVQ
                     YDRMKRQVEERVGKINGHFGRVAWTPVVYQYRHVPFSSLAAFYSVSDVCLVTPLRDGM
                     NLVAKEYVATRTDGTGVLILSEMAGSAKELPEAIIINPNNRSEIAHALKEALETPLEE
                     QKRRNKLMQRRLKRYHVTRWANDFLNNLVALREVQDRSEAKLLSPQARRTVVEQYRKS
                     CRRALFLDYDGTLTPLVKSPEMAKPDEDRLALLRSLAADPANDVVITSGRDRQTLQQW
                     FGDLRVGLVAEHGAWFKKSTEAWQLFKPFSLAWMRHLLPILEIYADRLPGAFVEEKEN
                     SAAWHYRAADPEQAGFLAAELTDHLLSLVSKTDLQVRQGSKVVEITNAGVDKGTAALK
                     WIIEKDYDFILAIGDDLTDEDLFRVLPDWAVSLRVGMAGTRAQHNLRSDVEVAELLQS
                     FAHTSGASLKAGKGSGRYSG"
     gene            6345..7139
                     /locus_tag="VF182_21810"
     CDS             6345..7139
                     /locus_tag="VF182_21810"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439768.1"
                     /translation="MRAHKKTNITVGDLVAAVTEEVEPLSGNKRRVNLLVSYILQDLF
                     LSCRVRLRNGFVNRQRARRQKWPAMALGLLLLVAPEASFAGAPSDQLKQSVEKIQTIL
                     ADPSLKGEAKSASRRQQLKEAVSERFAFDEMAKRSLGAQWQKRTASEQQEFVRLFTDL
                     LEGAYLSKLEEYSGEKIQFVNERQEKDFAEVNTKVVNRKGEDFALDYRLMNINGDWKV
                     IDVVIENISVVNNYRSQFNRMLSKSSFEDLTAAMKQKKISSPVASH"
BASE COUNT         1795 a         2125 c         1991 g         1750 t
ORIGIN      
        1 gatgatcaac gcgttctctc ccctcgatat caggccttcg atctgcggca gccagcgccg
       61 gttgcgatca tcgatcacct ttcgatggat ctcgggataa tcgcgcatac ccgcgagtag
      121 atgtttttcc aatgagccaa cgtcgccgct tttccaggcg cgcacgattt gcgctacatt
      181 ttgttccagg cggtccatct cgttcatgct ctggcgcagc aagagctctt gatcctttgg
      241 cgaaaatcga tcaaacaagt cgagctgaaa ttcagcagtt tccaagccga gggtgggttt
      301 gttggattga atggcacgct cggcaaagta tcgatcgaca cccaattttg gatcgaaccc
      361 cagcttttcg agttttaccg ccgccatcgt cgtggcgact atccagggct tgaaagaatt
      421 caataggcga atatcgagac ccagctcgtt ggctcttctg gcgacactct cgtaggtttc
      481 gtcggagaca ttccgctgca aaaaagcgcc gtccgtgctg gcactctttt gcagcatcaa
      541 ctgttggatc ctttcagggt tcgcgctgcg caagtcgatc tccaaaacca atttattcgt
      601 ctgctcgaag gcattctcga tgctctgttt gagcggatag gcctccttct tcagcaaatg
      661 aatcgagccc aggatataaa gactgttccg gtctgaatcc actttccaca agaagctttt
      721 ttcttgcgcg cttagctggc cggccaaagc aagagataga aaacagagcc cgagcagagt
      781 cagcaaacgt gactttttat aaagcttcat aaggtctgtg cttcaggagc ctgctccagc
      841 taacaggcaa tcgggagtcg gctatgtaga aatctacctc ttctcgcgaa acttgaccag
      901 cgctggactt agaagcttct atctccgacc ggcagtcgcg gaggtaacgt gaatatcaga
      961 acgcggatcg cctcagaagg aatccccgcg gcaaaaacga tgggcttatt taccattcaa
     1021 ggcgaatgca gacatcttcg cctttttcct cggctggaag gaccgtcaga ggcggcccgc
     1081 cttgaggcag caggcactca ccggttttca gatcaaaaca gtaaccatgg ttgttgcaac
     1141 gcaatttggt tccgtcaatc aattgtccgg cggattgcag gtccgcctct tcatgaggac
     1201 actgcttcga gaaggcaaag tatttcccct cgacgaacgt caacagcacc tgacgtccgc
     1261 cgaggtcgac ttctttcatg acgcccggcg ctaactcgcc gatccgagca atcggtatat
     1321 aactcactcc cattcggtta tgccgctacc ttctgctccc tgtagagagg accgcgcagg
     1381 acctggcccg gcagatcacc gctcatgcgg ctgtcttcat ggatcacagt gccattgacg
     1441 acggtgtagt gcattccaac cgaacgctgg atcaaccgct tgcttcctgc cggatagtca
     1501 tgggcccact ccggttcatg agaattgacg gtcttgggat cgaagatcgc caggtccgcg
     1561 gcataaccgg gtcttaacaa accgcgatcc tgcaagccga agatggatgc gacatggaat
     1621 gtgagcttat gaaccgcctg ttcgaggctc attacctggc gctcgcgcac ccacatacca
     1681 agcagaatcg tgcaatagcc aaagtccgcg ccgaactgca catgagcccc tgcatcggaa
     1741 gtgcctatca atacatacgg gctgcgcagg atctcgccca tggcgtcgac gtcgccgccg
     1801 ttgttggcat tccagaaaac cgtgccgaga tcttcctcga gcgccagatc caggaacgcg
     1861 tcgagtggat cttgattgcg catcgcgccc agctcagcga tcgtcttacc gttgtacttc
     1921 tcgttctccg gcttgaccac tttctcgacc cgaacgatgt tccagtttcg gtggaagttg
     1981 gtcggcgccg gattggcgag atcggcgcgc agcttctgcc tggtttggcc atcggcaaaa
     2041 gcctgcttgc gcacctcgac ggggaggaac atgagattct tccaggtcgg gaattcatcg
     2101 aaaacctggc agtttctaag ctcgaagtgg cgcaccagcg gcacggtgtg ggataagcca
     2161 taggcttgat agccttcgcg gaaaatcggc tctacagcat cgagctgttc acgccacaga
     2221 gtgggctctg cccatcgatg ctgcaggctc tgccagagaa ccgggcgttg ggtacgtttt
     2281 gccagctcat ggtagaactt gaaatgttcg atcttgttgc ggcccaaaat ggtttcgata
     2341 acgccgccgt tgtgctccgc caaaacatcg cacagcgcga acagctcatc atcatcggcc
     2401 aagcgactcg ccaccggctt gccgtcctcg cgcatatgac gctcgttgcg gttggtcgat
     2461 agccccaagg cgccgccttc catcgcttcc aagaccaagg cgcgcatctg ctggacttct
     2521 ttggcgctcg ccgtgcgctc cacggcctcc tcacccatga cattgtggcg catggcgatg
     2581 tggccgacca gcccgccgac attaataccg agtctcccct ccagcgcatc gaggtattcg
     2641 ccatagctgc tccagcccca cttcaccccc tgctcgagag cgaaccgtgg aattgcctcg
     2701 actcgaacaa aactttttac gatggcatcc tcatcacctt ttttgaccgg ggcaagcgat
     2761 aaaccgcagt tacccatgac tacgctggta accccgtgtt gcggttcaca ggtgccatag
     2821 ggatcccaca gcatttgagc atccaagtgc gtatgatgat cgatgaaacc gggagagact
     2881 gccaaaccgc cggcgtcgat ggtccgcgcg gctgtgccgt ttaaccgtcc aacttccacg
     2941 atcttgccgc ctttgacacc gacgtcggcc acataagacg gcaatcccga gccatcgacg
     3001 acgcgcccac cgcgaattaa caggtcataa gccatttgtg cctcctagtt gaacgaggaa
     3061 atcgtcaata ttccgaagcg agagtttgca cgtctaatac ggtaaccaac gctccacccg
     3121 atgaaccaac atgacatcct tacgccatat caattgcacc ccattttgtc catgatcaac
     3181 gctgccttct gtcgcctgga gccggtcccc tgcgtgtcac ggttccaatg atcgatgggc
     3241 gagttcgcct ccatgccctt cccaattttt gccatcgaat gagctgacgt tcacttgcct
     3301 tatgttcgtg gtgtcgaggc agcgaacatt aacgctgtag ccgtcgggat tagaacgcgg
     3361 aatataaaaa gacttaatgc cgcatgtctt acagaataga tgcttggcga cgccggtgtt
     3421 gaatgtatac gttgtaaggt ctgcggatcc ctggagcaac cggaaccgtg atcggggcac
     3481 gatcaagtga agaaagccgc ctttgctgca gatggaacaa ttacattcca gcgcatcgat
     3541 aatcgagact gcatcgatct cgaatcgcac ccgcccgcaa tgacaagcgc ccgtatgctg
     3601 aactaattct tccatcaaaa cccctgcagc gctgccgcaa ccggcgccta gcccgaataa
     3661 cggcctgaac ctttacccgc tttgagcgac gcgccggaag tatgggcgaa tgattgcagc
     3721 agttcggcca cctcgacgtc gctgcgcagg ttgtgttgcg cgcgggttcc ggccattcca
     3781 actctaaggg ataccgccca atcgggcaaa actcggaaca agtcttcgtc ggtcagatcg
     3841 tcgccgattg ccaggatgaa atcgtaatcc ttttcgatga tccattttaa tgcggcggtc
     3901 cctttgtcga ccccggcgtt ggtgatttca accactttgc ttccctgcct gacctgcaga
     3961 tcggtctttg agaccaggct caacagatga tccgttaatt cggcggcgag aaagccggcc
     4021 tgctccggat ccgccgcgcg ataatgccag gcggcggaat tctccttttc ctcgacaaag
     4081 gcgccgggca atcggtctgc gtatatctcc aatatcggca acagatggcg catccacgcc
     4141 agggagaatg gtttgaaaag ttgccacgcc tctgtcgact tcttgaacca tgcgccatgc
     4201 tcggcgacca acccgactct gagatcgccg aaccactgct gcagagtctg acgatcgcgt
     4261 ccgcttgtga tgacgacgtc attagccgga tcggcggcaa gagagcgcaa gagcgccaga
     4321 cgatcttcat ccggtttcgc catctccggc gacttcacca agggagtcaa ggtcccgtca
     4381 taatccagaa acagtgcccg gcggcaactc tttctgtact gttcgacaac cgtccggcgc
     4441 gcctgcggtg aaagtagttt ggcttcgcta cgatcttgga cttcgcgcaa ggcgacaagg
     4501 ttgttgagaa aatcattggc ccagcgagtg acgtggtacc gttttaagcg gcgctgcatc
     4561 agcttgttgc gccgtttctg ctcctcgagc ggtgtttcga gcgcctcttt aagagcgtgg
     4621 gcgatttccg accggttatt cggattgatg atgatggctt cgggcaactc tttggccgaa
     4681 ccagccatct cgctcaggat caaaaccccg gtgccgtcgg tccgggtagc cacgtattct
     4741 ttggcgacta aattcattcc atcacggagc ggtgtaacca gacacacatc gctcaccgaa
     4801 tagaacgccg ccagtgatga gaacgggaca tgacggtact gatacactac tggagtccaa
     4861 gcgactcggc cgaaatgccc attgattttg ccgacccgtt cttcgacctg tcgcttcata
     4921 cgatcgtatt gcacaactcc gatgcgcgag ggcacgacaa tcatcaggag aacgactttg
     4981 ccatggaatt gcggattgtt ttccaggaat aactcgtagc cttccagacg gtgcaaaatc
     5041 cctttggaat aatcgaggcg atcaaccgac aagatcatct tgaactcgga aagtgtgctg
     5101 cgcagcgcct gtatttcctg ctgggtatca ggctccgcag gcgcattgga gtatttgtcg
     5161 aaatcgattc ccatcgggaa cgtgtcgatc ttgacaaggt gatcgggcgt caatagctgc
     5221 gccatctgat gctcataacc gaggatgcgt agaacgcact ggagaaaatg gtgcgtatac
     5281 tcgtaagtat gaaagccgat taaatcagcg cccagcaatc catcgagaat ctcgcgacgc
     5341 cattctccgg gcagcagacg aaacacctcg aatgagggga acgggatatg cagaaaaaac
     5401 ccgacggaag cttgaggccg ccgggctttg atcagccgcg gcagcaacat caagtgataa
     5461 tcatgaatcc ataagatgtc attgggtctg acaacgtcgt ccagcgcatc acagaagttt
     5521 tgattgatct gtttgtactc ctcccagtag ttgggccgat attcggtaaa agtaggaaag
     5581 tagtgaaaca gcggccaaat cgtcgaattg caaaacccga gatagaactt ctccatctgc
     5641 tcttcgctga gaaagaccgg gtaagactga aaggaactga gcgcctcgcg gatcaactgg
     5701 ccctggagag agacctcgac cgtgcttccc ggccaaccga cccagagatg ctccatgctc
     5761 aaagccggag ttcgtctgcg cgattgcatg aatgacgcca agcccgtaac cagaccgccg
     5821 gcgctctcgt gaaaggttat ctttccgttc tcgaccttga cattgaaggg caggcgattg
     5881 gagacgataa cgagtcgtcg ctcggacaat gtcgaatgta tcaagctcat ttgtggcttg
     5941 ttttgaatca ccaatccatg cctcagctcg ggcctcgtgg ggaaaaaagg cgcgcgcgac
     6001 tttgctttag aacaatatca cttcagcggc gcttgtcacg cacataaatg cctgatgtct
     6061 gtagcttaag taatcagcgc cagtcacact ttgatcgagc aatcaccgtc gagtccagcc
     6121 tattgtatct cgaacttgct ttgctgcctg aaaaaaaacg actgaaaaag ccttcgttgc
     6181 tttttggttg agactcgccc gggcagcaca aactttcttt aaatttaagg agatagctct
     6241 gacctacggg attatggcat atccgttgct cgttcaaccg ttcactttat ttcccctatt
     6301 ttgaaatcgg tggtagattg tcggcgggaa gaggagggtc ttacatgcgc gcgcacaaga
     6361 agacaaacat cacggtaggc gatctcgttg ccgcagtgac cgaggaagtc gagccgctgt
     6421 ccggcaacaa gcgccgggta aaccttctgg tctcatacat tctccaggat ctgtttctga
     6481 gctgtcgggt ccgacttaga aatggatttg tgaaccggca acgcgccaga cgccagaaat
     6541 ggccggctat ggcgctggga ttgcttttac ttgtcgctcc cgaagcaagt tttgccggtg
     6601 cgccgagtga ccaactaaaa caatcggttg aaaaaattca gactattctg gctgatcctt
     6661 cgttaaaagg agaggccaag agcgcaagcc ggcggcaaca gctcaaggaa gccgttagtg
     6721 aaagattcgc tttcgatgaa atggcgaaac gttcattggg cgcccagtgg caaaaacgca
     6781 ccgcgtcaga gcaacaggag tttgtccgat tgttcacgga cctcctcgaa ggcgcctact
     6841 tgagcaaact tgaagagtac agcggcgaaa aaatccagtt cgtcaacgag cgccaggaaa
     6901 aggactttgc cgaggtcaat acaaaggtcg tcaacagaaa gggtgaagac tttgcgctcg
     6961 attaccggct tatgaatatc aatggcgact ggaaggtaat cgacgttgtc attgaaaaca
     7021 ttagcgtagt taacaactac cgctcacagt ttaatcgcat gctgtccaaa tcctctttcg
     7081 aggatttaac ggcggcgatg aagcaaaaga aaataagctc gccggtagca agccattagc
     7141 actgggaaga attgccactg attctgccgc ttcgcagagt tggcgatatc gcgtacatca
     7201 acagattaga agcgctctct gcgggccata gccggctttc gggagcgcct tcagcagcaa
     7261 aacaaatcct ctaacattct cctactcgcg gcgccgttga aacataaaaa agcgttcgat
     7321 ctcatcgagg agccagattc gctgtactat ggatagcatg agtttttcgg cgacagctcg
     7381 ttcgatcggt atcttatcgg atcgcgggat gataagatca tagtactacc agccagatga
     7441 caaactcctt agtggttctt tgaaaccaac aaggacatgc ctcgatttat cgccgccggt
     7501 ctcgttggag gcgcaatttg ctttgagaat atcgtgggcc cgaccgctgg attatgcctc
     7561 gcttaagttc cagcagaatg gagaattcac atgatgttaa ctgttggagc taaggtcgtt
     7621 tacccgagcc gcggtccttg cctgatcggc gaggtggtga a
//
LOCUS       DASUVS010000407         3488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_181402, whole genome shotgun sequence.
ACCESSION   DASUVS010000407 DASUVS010000000
VERSION     DASUVS010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181402"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VF182_21815"
     CDS             complement(<1..546)
                     /locus_tag="VF182_21815"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6439769.1"
                     /translation="MALNSEKKPRVTCITLEHPPVNVLGIELLRELDQELSACAAEKT
                     TDVVVLRGGGTRAFSAGMDIRDHTREKVPEMLEIVHRVIRKLLTMPQVTIAAVRGVCL
                     GGGCELASSCDFIVASEDSSFATPEIQVGCYPPVALARFSTLIGYHRAAEMILTGRHY
                     NAREALDLGLVNRVFPQDQFDN"
     gene            695..1456
                     /locus_tag="VF182_21820"
     CDS             695..1456
                     /locus_tag="VF182_21820"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX6439770.1"
                     /translation="MKNSRLLAVTLAAWFALGMRPVFAAPDEYDDSQSHPLRIVAYLL
                     HPAAWLVEQVVFRPFHYLVSANEPTEEIFGHRPHPPVLAERQPVRDYGMPPRTPVKPA
                     PPPAPVAAAPEPVREVVKVVEVPKETVVYRDVEKVVEVEKFVFPGIAFQFDSAELTDW
                     GKGNVYLAAEKLKAKGDISVIIEGHTDEVGSAEYNKNLGLRRAQRVMTELAALGVDRA
                     RISTATQGEDKPLINQPTAWARAVNRRVEFEVRGK"
     gene            1530..2135
                     /locus_tag="VF182_21825"
     CDS             1530..2135
                     /locus_tag="VF182_21825"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX6439771.1"
                     /translation="MSYKLRDTYYKKAKQQGYRSRAAYKLLELQERFRIFKTGHLVVD
                     LGAAPGGWIQVAAKLVGPSGKVIGVDLQPIEPFHQKNIIVVHADITAVETEQRIMEYL
                     GRPADSVISDVAPKLTGIRDTDEARSLELNHTAWEIAKRLLRSGGSFLIKSFVSEELR
                     NFSAKLEQQFGSVQRTRPDASRKGSSEIYFFAKDYQPKPQR"
     gene            2197..3198
                     /gene="galT"
                     /locus_tag="VF182_21830"
     CDS             2197..3198
                     /gene="galT"
                     /locus_tag="VF182_21830"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEX6439772.1"
                     /translation="MANSELRKDPVVDRWVIIATERSKRPHANRTTTVSGRDEGCPFC
                     AGCEGMTPPEVLAYRDPSSNPNAPGWRVRVVPNKYPALDSVAELFQPSDSPYVALNGI
                     GAHEVIIESPKHCLSMADLGEEQVEDVLRAYRTRLIVLQKDQRWKSVLIYKNEGSAAG
                     ATLEHVHSQILALPIVPREIDQEWRVLIAHYEATERCLYCEMVDKERADGRRIILESA
                     AFIAFCPFASRFPFEICLMAKEHSPGFDAVANEELPQLACMLRQSLQRLAGIVDAPLN
                     YVIHSAPLRDLKREHYHWHLEILPRISKIAGFELASGYYINTVAPEVAAQQLRDVAL"
     gene            complement(3255..>3488)
                     /locus_tag="VF182_21835"
     CDS             complement(3255..>3488)
                     /locus_tag="VF182_21835"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010597919.1"
                     /note="Catalyzes the first step in hexosamine metabolism,
                     converting fructose-6P into glucosamine-6P using glutamine
                     as a nitrogen source; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEX6439773.1"
                     /translation="NLKEVESRGGKVIVLTDDIKTSHEVSAYRLLTLPKASHYLTPIV
                     MTIPLQLLAYHIAVHRGTDVDQPRNLAKSVTVE"
BASE COUNT          821 a          929 c          964 g          774 t
ORIGIN      
        1 gttgtcgaat tgatcttggg gaaagacacg gtttaccaat ccaagatcaa gcgcttctcg
       61 agcattgtag tggcgcccgg taagaatcat ttcggcagcc cgatgatatc cgatcaatgt
      121 cgagaaacgc gccagcgcaa ccggcggata acaacccact tgaatttctg gggtggcaaa
      181 tgaactatct tccgaggcga cgataaagtc gcaggaactc gcaagctcgc aaccgccgcc
      241 gagacatacg ccgcggaccg ctgcaatggt tacctgtggc atagtcaaca atttgcgaat
      301 gaccctatga acgatctcaa gcatctcggg aaccttttcc cgggtgtggt cgcgaatatc
      361 catgccggca gagaaggcgc gtgtgccgcc gcctcgtagg actacgacat cggtcgtttt
      421 ctcggcagcg catgccgaca attcctggtc cagctcacgc agcaactcga tccccagaac
      481 gtttaccggc ggatgctcaa gtgtaatgca ggtcacccgt ggtttttttt ctgaattcaa
      541 tgccatgtta tccctatagc gtccaatctg ataaaacgcg agtgctcgag agctgtaacc
      601 aatggcaacg aagcatgtct atcggcacaa ctgatttgac aaagaaaaca ctgcaaccta
      661 gacttgtggt tctgagctcc taaggagggc ttttatgaag aattcacgct tgctagctgt
      721 gactcttgcc gcctggtttg cgttaggaat gagacccgtt tttgctgctc cggatgaata
      781 cgacgacagc caatcgcatc cattgagaat cgtcgcctat ttgcttcacc cggctgcgtg
      841 gttggtcgaa caagtagttt ttcggccttt tcattatctc gtcagcgcga atgagccaac
      901 ggaggaaatc ttcggccatc gaccgcatcc acccgttttg gccgagcgcc aaccggtcag
      961 agactacggc atgcctccgc gaacacccgt caagcccgca ccgcccccag ctcctgtagc
     1021 ggccgctccg gaacccgtac gcgaggtcgt gaaagtcgtc gaagtgccaa aggagacggt
     1081 cgtatatcgg gacgttgaga aagtcgttga agtcgagaag ttcgtttttc ctggcattgc
     1141 ttttcagttc gattcggccg agctcaccga ttggggcaaa ggcaatgtct atcttgccgc
     1201 ggaaaaactc aaggctaaag gcgatatctc tgtaatcatc gaggggcata ccgatgaggt
     1261 cggtagcgca gaatacaata aaaacctcgg cctgcgccgg gcgcagcgcg tcatgaccga
     1321 gttagccgct ttgggagttg atcgagcgag aatttcgaca gcgactcagg gtgaagacaa
     1381 gcctctgatc aatcaaccga cggcctgggc gcgcgcggta aatcgacgag tagagttcga
     1441 ggtccgaggg aaataaaaac atttttcaaa cgttcaaggc ccagcttcag gctgggcctt
     1501 tttttttgag taccacagtt ctcgatcaaa tgagttacaa gcttcgcgac acctattaca
     1561 aaaaggcgaa acaacagggc tatcgctcgc gcgcggctta caagctgcta gagctgcaag
     1621 agcgctttcg aatcttcaaa accggccact tagttgtcga tctaggcgcc gcgcctggcg
     1681 gctggatcca agtggcggcc aagctggtcg gcccgagcgg taaggtcatc ggcgttgatc
     1741 ttcagccgat cgaaccgttt caccagaaaa acatcattgt cgtacacgct gacatcactg
     1801 cggtggaaac agagcagcgg attatggaat acctaggccg ccctgctgac agcgtcatct
     1861 ctgatgtcgc gcctaagttg accggcatcc gtgatacgga cgaggcgcgc tcgctggagt
     1921 tgaaccatac agcctgggaa atcgcaaagc gcttgcttcg aagcggcggt agctttctta
     1981 tcaagagctt cgtcagtgaa gaacttcgga atttttccgc gaaactggag cagcaatttg
     2041 gctcggttca gcgtacccgg ccggatgcca gccgcaaagg atcatcggaa atttatttct
     2101 tcgcgaaaga ttaccagccc aagccccaga ggtagacgaa tgggtcgtgg tcaagacttc
     2161 agacgctcac cggtaaacca agttcaaaaa cgataaatgg caaactcgga gttaagaaag
     2221 gatccggttg tcgaccgctg ggtgatcata gccactgaac gcagcaaacg gcctcacgcc
     2281 aaccggacaa cgacagtgtc cggccgagac gaaggctgtc ctttctgcgc gggctgcgaa
     2341 gggatgacgc cacctgaagt tctcgcttat cgtgatccct cgagtaatcc aaatgcgcct
     2401 ggctggaggg tccgagtagt ccccaataag tacccagctc tggacagcgt ggcagagctc
     2461 ttccaaccaa gtgattcgcc atacgttgct ttgaacggaa tcggcgcgca cgaggtgatc
     2521 atcgaaagcc ccaaacactg cctgagcatg gcagatctgg gtgaggaaca ggttgaagat
     2581 gtgttgcgcg cgtatcgaac ccggctgatc gttcttcaga aggatcagcg ctggaaatcg
     2641 gttctaattt acaagaacga aggaagcgcg gcgggtgcga ccctggagca tgtccattcg
     2701 caaatacttg cgttgcccat cgttccgcgt gaaatagatc aagagtggcg cgtgctgatc
     2761 gctcactatg aggcgaccga gagatgcctc tattgcgaga tggtcgataa ggagcgcgcg
     2821 gatgggcgcc gcataattct cgagagcgcc gcgtttatcg ctttctgccc gttcgcgtct
     2881 cgatttccct tcgaaatttg tcttatggcg aaagaacact cacctggatt tgacgcggtg
     2941 gcgaacgagg aactccccca attagcatgt atgctgcggc aatctttaca gcgtctggcc
     3001 ggtatcgtcg atgcaccgct caactatgtg attcattcag cgccgcttag ggacctcaag
     3061 cgagagcatt atcactggca cctggagatt ttaccgagga tttccaagat cgccgggttc
     3121 gagctggcct ccggctacta tatcaatacc gtagcgccgg aagtcgcggc acagcagctg
     3181 cgcgacgtcg cgctatgaca agcctgccgt tctgatttgc gccgcatgtc tcacgatgtc
     3241 tctcaggaga gtctttattc gacggtcaca cttttcgcca ggttgcgcgg ctgatcaacg
     3301 tcagtgccgc gatggaccgc gatgtgatac gccagcaact ggagcgggat cgtcatgacg
     3361 atgggggtca ggtagtgcga tgctttcggt agtgttagga gccgataagc ggagacttcg
     3421 tggctcgtct tgatatcgtc ggttagaacg atcactttac cgccgcggga ttcgacttct
     3481 ttgagatt
//
LOCUS       DASUVS010000408        13894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1493834, whole genome shotgun sequence.
ACCESSION   DASUVS010000408 DASUVS010000000
VERSION     DASUVS010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13894
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493834"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VF182_21840"
     CDS             complement(<1..1178)
                     /locus_tag="VF182_21840"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6439774.1"
                     /translation="MSLLDAAIKGLMNILHLKVFLAMIVGVSIGTFTAVAPQGLGMPL
                     VYAILLPVVVRWEPVTGMALLIGASSVSAICAAYLPILFGIPGGSGSQATVLDGYPMG
                     KRGEGRRALGASFMAGGMGCLVGTFTLAVAIPLAQPLIYLMGAPELFVVVLWGLSMVA
                     VLAGRRPIKGLIAAAFGLLIATVGQQGQSGIMRFVFDQPYLLDGIPLSIIALALFGIP
                     SALDLALTRIGVEQQAAPLKGSLLDGVKDTLREWWLVLRCSFIGVWVGIVPGIGSQVV
                     DWLAYGHAAQTSKDAKNFGKGDVRGVIAPESANDAKDGGDLVTTLLLGFPQGVVTALF
                     IVALLAWGYLPGPEMVKKNLDIIYSVVWSQGISGVIGTLIGFVLAAQLAKLAEVRYSV
                     "
     gene            complement(1213..2280)
                     /locus_tag="VF182_21845"
     CDS             complement(1213..2280)
                     /locus_tag="VF182_21845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439775.1"
                     /translation="MLKSRFLTSVGLILIFLFASTLFAAAGFAQAPFYQGKTITMIVS
                     TAPGGTGDLRVKAMAPVLRRHIPGNPAIAMEYMDGGGGRKAANYLFRNSRPDGLTIGA
                     LSGGIVSLEIMRESGVMYDLDKFIYLGAPESGGHYIIYTRKELGLSSIDKLRAASGLR
                     IGAQSVGHVSYVAGRLFAYFLGLKDPKFIAGYSAPEVDAALLRGELDGRANLATSMLR
                     RNPEWVDKDMMDFHSMMEVPLGAKHPRFPTVPEIETFAKSEQEKKVLAVWRVFRLVGS
                     PYMLPPGTPKDKVEILQEAMRKTLKDAQFHREYKKLVGDDVDPLMPEELSKVIRDIPR
                     DPEVIEMLRTLSGAGPLPARS"
     gene            complement(2303..3379)
                     /locus_tag="VF182_21850"
     CDS             complement(2303..3379)
                     /locus_tag="VF182_21850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439776.1"
                     /translation="MSRRESSFTSVARLLVIAVFLYSFLSAAEVLAQASFFQGKTLTI
                     VQGRDPGGSGDLRVRALVPFLEKYIPGNPKIVMEFMPGGGSRKAANYLFRTAKPDGLT
                     IGNLSGGMVSLAVLGESGILYDLDKFYYLGSPYSTYHAILVSRKEAGLDTIEKLRATP
                     GLKLGAQSVGFSTFLEARVFAYILGLRDTSFIAGYSGVELDPALLRGEIDLRSNGADT
                     IVQRNKDWLKGGIVNFHAITETPKGEKHPAFPNLPEIETFAKSALDRKIIAMQRAFRV
                     TGTPFVLPPATPKDRVQTLQDAFRKTYKDPEFAKHYQKLTSDEPSPLLPEDHEKAIRD
                     VPRDREAVEVFKRLANAVALPARR"
     gene            complement(3851..6163)
                     /locus_tag="VF182_21855"
     CDS             complement(3851..6163)
                     /locus_tag="VF182_21855"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6439777.1"
                     /translation="MTTEHNGGGLIGASIRRVEDPLLISGKGGYVDDIKLPGMLHMMV
                     LRSAYPHAKIISINTDAAKAMKGVVAVVTGPELPERLNINAQVMFPGQKIPPHPVLAR
                     GAVHAPGTPVAAVVAESRAIAQDACNAIEVEYQALPSIQNAEDALKPGAPLAREELDS
                     NICYTAVKKGGDVDKAFAQADHIVRMHIVSPRQVALAIEPRGVAANPDPMGKRLTVWL
                     STQGPHRARADLAVTLGIAENKIRLIAPDVGGGFGSKGPLYREDVLTSYMALKLRRPV
                     KWIATRSDDFVTTIQGRDQAMTSELALKKDGTMLGLKVRVVANLGGYLQSSTAGPPQR
                     MMGMAPGCYQIRDCHVEVVGVFTNTVPTGPYRGAGRPESVLNIERLIDKAAKDLGIDR
                     LEIRRKNFIRPEQFPYKTAVGVEYDSGDYFKSLDEAIKIANYQELLRQRDERRARGEI
                     VGVGVSTFVEPSGGAGFESGTVRIERTGEITVLTGSSSHGQGHETVWAQIAAEMLKTD
                     MEHVTVLHGDTYVSQQGTGTFGSRSAVVGGGALATAAQRVIEKAKRIAAHLVEASAED
                     MVQADGGFSVAGVPEKKVTWRQIAAAAYGGKIPQGMENGLQETCFFDPRREAWGFGAH
                     VCLVSINRETGEPKIEKLVLVDDCGVLINPMIVEGQIHGGVAQGLGEALREQMVYSED
                     GQVLTGTLMNYAVMRATDMPRLILGETVTPNPFHPLGVKGVGEAGTNGAPPAVANAVM
                     DALAPLGIDHIDMPYTAPKLWQAIRQAGGT"
     gene            complement(6340..7209)
                     /locus_tag="VF182_21860"
     CDS             complement(6340..7209)
                     /locus_tag="VF182_21860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6439778.1"
                     /translation="MFPASFGYLAPQSLDEALQLLKQHGDDAKLLAGGHSLLPAMKLR
                     LASPRTLIDLGTVPGLTGVRLEGDKLMIGALTVHADVASSDVVGKYFPGLVDAASVIG
                     DVQVRNRGTIGGSVAHADPAADFPVILTALNASFVLRSIGGNRTVPVDDFFTDYFTTA
                     MAPGEILTEILLPLPPVHSGSAYHKLAHPASGYVVVSAGAMITRQQSGSCAAARIAIG
                     GLGSGPIRAVASEKELQGNVLNPQNIVAAAAKAAEGADPDDDIYASAEYKRHMATVLA
                     RRAIEAAVQRAAN"
     gene            complement(7196..7702)
                     /locus_tag="VF182_21865"
     CDS             complement(7196..7702)
                     /locus_tag="VF182_21865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6439779.1"
                     /translation="MTAEAEYAALPITVTVNGVKHEAMVEPRTLLVYFLRDQLGLTGT
                     HVGCDTSQCGACTIHLNDRAVKSCTILAVQANGKKITTIEGLAHDDEFHPVQEGFREK
                     HGLQCGYCTPGMIMTAADLLRTNANPSEEQIKHALEGNLCRCTGYVNIVESIKWAAEK
                     IGAKNVSR"
     gene            7963..9252
                     /locus_tag="VF182_21870"
     CDS             7963..9252
                     /locus_tag="VF182_21870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX6439780.1"
                     /translation="MVEIKSLIDPEGGLIDRRIFADREIYQLERERIFARCWLYLGHE
                     CEIPNPGDFVTAYMGEEPVILWRDLNRQVHAYLNVCRHRGNRVCRADRGNARVFTCSY
                     HGWTYSSEGKLAIVPMPEAFPGLDREQWGLIPVAQLSAYKGLIFATFDPSAPSLLDYL
                     GDMAWYLDILLDRRQGGTEVSGPHRWIVDANWKTAAENFGGDGYHISYTHGSARELGV
                     DTTSSSTRKWNKGCQIACGNGHILVSWLTPPDDAGPWFAQPDEELLNYMKECAGEIEQ
                     RLGKVRARQISPSAGTVFPNFSVHWLTRTIRVWQPRGPDKMEIWSWAIVDKAAPPKIK
                     DRMRFVSQYRFSPSGIFEQDDMDNWIQVTGAAKSIIARRYPANYQMSGSEEIIQPELP
                     GRLASRFSDNNQLNLYMHWAKMMEANSWNEIAGAERA"
     gene            9255..9764
                     /locus_tag="VF182_21875"
     CDS             9255..9764
                     /locus_tag="VF182_21875"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HEX6439781.1"
                     /translation="MNHDLRHAIEDFFYFEAELLDERRLRDWLDLLTDDVQYWMPVRH
                     NPLERSADLNDELSKPGDVYYFNDTKDTLKVRVERVYAKNAWAEMPPSRTRHLVSNVR
                     VKNDNGSEIEVQSNFLCYRTRMEKDQDLFVGTRHDLLRRAHGNFRIARRKIILDQANL
                     AAKNISIFL"
     gene            complement(10141..11022)
                     /locus_tag="VF182_21880"
     CDS             complement(10141..11022)
                     /locus_tag="VF182_21880"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX6439782.1"
                     /translation="MSSSLPIKLIEKQHWLEPIEEQLQPTIAAAFDDNGSIGPKVGNI
                     LHGTWLGHPLHVVLTDVPIGSWTAAAVFDLLEQTSGSPAMGRKADAAIAFGLIGATAA
                     AVTGLTDWSKVGPSRARRIGLAHGVLNLAATACYVTSLCLRRVHSRNAGRQFAMAGFM
                     ISNLSAYLGGHLVYNERIGVDHTAGFSPPKDFFPVLTESELSDNELRRVDADGMPVLL
                     VRRGMRIYAIAETCSHLGGPLAEGELQDTVVKCPWHGSCFSLENGRVVNGPSVHPQPV
                     LEVRVRDGQIEVRGSNR"
     gene            11563..13155
                     /locus_tag="VF182_21885"
     CDS             11563..13155
                     /locus_tag="VF182_21885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein D"
                     /protein_id="HEX6439783.1"
                     /translation="MRRRDFLKSALILTGAPLSIPALRPSAGRGQPVGAENFNYDWLK
                     SHARRLSSTQYQAPDRVLPNTLRQLDYDGYQAIRFDSDKALWARESLAFRIEFFHRGP
                     MFKEPVRMHEIVGGQASAILYHPAMFNFRRSPVDPGALSQDLGFAGFRVHFHTDWRAD
                     VAAFLGASYFRAVGSDYRQYGISARGLAVDTALDKGEEFPRFTDFWLERPVKHSQQLT
                     IYALLDSPSVTGAYRFEIRPGTTLIMDVDAAIYPRKMIERIGIAPLTSMYQHGENDRR
                     MATDWRPEIHDSDGLQLLTGNGEWIWRPLVNPSGIRVNSYLDTNPRGFGLLQRDRNFD
                     HYQDDSVFYERRPSLWVEPKIDSSGGWGTGAVQLAELPAPDETFDNIVAYWTPAAQPQ
                     PGQELLFSYRLYWGSRIPAAPALAQVVATRTGIGGIVGQPRKYFSYRFAVDFSGGKLA
                     TLSKAAQVEPVISASRGEIEIASARPQLEIGGYRAMFDLKPIDDTTEPIDLRLYLRHG
                     GQPLTETWQYQWRPPPVDERKL"
     gene            complement(13195..13398)
                     /locus_tag="VF182_21890"
     CDS             complement(13195..13398)
                     /locus_tag="VF182_21890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HEX6439784.1"
                     /translation="MAKYGPKAKQKVKKAMHERKKGTLKSGGSGKKVTSRKQAIAIGL
                     SEARRAGGKVPRKKSSTRKVKKK"
     gene            13700..>13894
                     /locus_tag="VF182_21895"
     CDS             13700..>13894
                     /locus_tag="VF182_21895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439785.1"
                     /translation="MNATQHMLASARALGAERMRLLGILTGGHFVIHWFQQFFPVILP
                     SIKSGLSLTNIEVGALTSAQQ"
BASE COUNT         3028 a         4018 c         3774 g         3074 t
ORIGIN      
        1 atgaccgagt aacgcacctc ggcgagtttg gccaattgcg ccgccaaaac aaagccgatc
       61 aaagttccga tcacgccgga aatgccctgg ctccaaacca cgctatagat aatgtcgagg
      121 ttctttttaa ccatttccgg gcccggtaaa tatccccagg ccagaagcgc gactataaag
      181 agcgccgtga ccactccttg aggaaatccc agcagcaacg tcgtcaccag atctccgcca
      241 tccttggcgt cattggcgct ctcgggcgca atcacgcccc ttacatcgcc cttgccgaaa
      301 ttttttgcgt ccttgctggt ttgcgcggca tgaccgtagg caagccaatc gaccacctgc
      361 gagccgatgc cgggaacgat gccgacccag acgccgatga agctgcagcg caaaaccagc
      421 caccactcgc gcagggtgtc cttgacgccg tcgagcaggc tgcccttcaa aggcgcggct
      481 tgttgctcta cgccgattct cgtcagcgcc agatccaggg ccgacggaat gccgaatagc
      541 gccaacgcaa tgatgctcaa ggggatgccg tccagcagat acggctggtc gaagacaaaa
      601 cgcataatac cgctctggcc ctgttgaccc acggtcgcga tcagcaagcc aaatgctgct
      661 gcgatcaagc ctttgatcgg ccggcgtcca gccagaactg caaccatgct gagaccccac
      721 aacaccacca cgaacaactc cggcgcgccc atcaaataga tcaatggttg cgccagcggg
      781 attgccactg ccagggtaaa ggttccgacc agacaaccca ttcctccggc catgaaagag
      841 gcgcctaacg cgcgccgtcc ttcgccccgt ttgcccatcg gataaccatc gagcacagtc
      901 gcttgggaac ctgagccgcc ggggatgcca aatagaatcg gcaagtaggc agcgcaaatc
      961 gcgcttacgg agctcgcgcc gatgagcagc gccataccgg tgaccggctc ccagcgcacg
     1021 accaccggca ataggatcgc gtaaaccagg ggcatgccga gaccctgcgg cgctactgca
     1081 gtaaaggtgc cgatgctaac gcctacgatc atggcgagaa aaactttcag atgaaggatg
     1141 ttcatcaggc ctttgatggc ggcatcgagc agtgacatct ccattttccg ttcccgtctt
     1201 gcttttcttc aatcaactac gcgctggaag aggaccagcg ccggatagtg ttctcagcat
     1261 ctcgataact tcgggatcgc gcggaatgtc ccgaatcacc ttgctcaact cttcaggcat
     1321 caatgggtcg acgtcatcgc cgaccagctt cttgtattcg cgatgaaact gagcgtcctt
     1381 gagagtttta cgcatcgcct cctgaagtat ttcgactttg tcctttggag ttcccggcgg
     1441 cagcatgtaa ggagaaccca ccaacctgaa cactcgccaa acagcgagga ctttcttttc
     1501 ttgctccgat ttggcgaagg tctcgatctc gggcacggtc gggaagcgcg gatgtttggc
     1561 gcccaagggc acctccatca tcgaatggaa atccatcatg tccttgtcca cccactccgg
     1621 gttccggcgc agcatcgaag tggcgaggtt cgctctgccg tccaattcac cgcgaagtag
     1681 cgcggcatcg acttcgggcg cggagtagcc ggcgataaac ttgggatctt tcaagccgag
     1741 aaagtaagcg aagagtcttc cggccacgta ggaaacgtgt cccaccgatt gcgcgccgat
     1801 gcgtaggcct gaggctgccc gcagcttgtc tatgctgctt aagcccagct ctttgcgtgt
     1861 atagatgatg tagtggccgc cgctttccgg agcgccaaga taaataaatt tgtcgagatc
     1921 atacatgacg ccgctttcgc gcatgatctc cagggaaaca atgccgccgc tcagcgcgcc
     1981 gatggtgaga ccgtcaggac ggctgtttct aaacagatag ttggcggctt ttcggccgcc
     2041 gccgccgtcc atatactcca ttgcgatagc tggattaccc gggatgtgcc tgcgcaggac
     2101 cggagccatg gctttgaccc gcagatcgcc cgtgcctccg ggcgccgtgc tgacgatcat
     2161 ggtaatggtt ttgccttgat aaaacggcgc ctgtgcgaac ccggcggcgg caaacagggt
     2221 cgatgcgaag agaaagatta ggattaaacc gacactcgtt aaaaatctac ttttcagcat
     2281 tgcttctccg cagaatcgac cgctagcgac gcgccggcag cgcgaccgca ttggcgagtc
     2341 gcttgaaaac ttccacggct tcccgatccc gcggcacatc tcgaatcgct ttttcgtgat
     2401 cctcgggcag cagcgggctc ggctcgtcgc tggtaagttt ctggtaatgt ttcgcaaact
     2461 ccggatcctt gtaagtcttg cgaaacgcat cctgaagagt ttgcacgcga tccttgggtg
     2521 tggccggcgg caaaacaaat ggcgttccgg ttactctaaa ggcgcgctgc atggcgatga
     2581 ttttgcggtc gagagcgctt ttggcaaagg tttcgatctc cggcaggttc gggaaagccg
     2641 gatgcttttc acctttggga gtctcggtga tcgcatggaa attgacgatt ccgcctttta
     2701 accagtcttt gtttctttgc acgatggtgt ccgcgccgtt ggagcgcagg tcgatctcac
     2761 cacgcaacag cgccgggtcg agctcgactc cggagtagcc ggcaatgaag ctggtatctc
     2821 tgagcccgag aatataggcg aacacccgcg cttcaagaaa cgtcgagaag cccaccgact
     2881 gcgcgccgag tttaagcccg ggagtggcgc gaagtttttc gatggtgtcc aggcccgcct
     2941 cttttcggct caccaatata gcgtgataag tgctgtaagg agaaccaagg taatagaatt
     3001 tatcgagatc gtagagaatg cctgactcgc cgagtacagc aagcgagacc ataccgccgc
     3061 tgagattgcc gatcgtcaac ccgtccggct tggccgtacg aaaaagatag ttggcggctt
     3121 tacggctacc gccgccgggc ataaactcca taacgatttt cgggttaccg ggaatgtact
     3181 tttccaagaa cggaaccaga gctctgaccc gaagatctcc ggaaccaccg ggatccctgc
     3241 cttgaacgat cgtgagcgtc tttccctgaa agaaagaggc ctgggcaagc acctcggctg
     3301 cggacaggaa cgaatacagg aatacggcaa tcaccagcag ccgtgccaca gatgtaaagc
     3361 tcgattctct tcgcgacata tactctcctt actaaaagca acgccgtatc ccgaaacggg
     3421 atggattcaa ttacggttcc ggttatttgt caacggccag tataaaaggc catccaactt
     3481 gccgatgtac caacaccagg ttctttatta gcgagatttc gttccgcagg tgaggaaatc
     3541 tggctcgatt agaatcaagg atatgtggaa atgacaaagc actcgagcgt gttaactaaa
     3601 acggcccgat ctcgtaacaa ggatctttag tgaacagcac gcagcagatt gcgatcatca
     3661 aacccgcaat gattgaaagt tggcagggag aatccattcc aacaaactcg accaaagaaa
     3721 actcccattt tctgacaacg ttttttcgct tcgaccatca cgatttgagc ggctttgcaa
     3781 caggagaaac gcacccgcac gcggtcgtag gttcttccac ggacacgagc acccgccacg
     3841 attacagttt ttaagttcca cctgcctgac gaattgcctg ccagagcttc ggcgccgtat
     3901 agggcatgtc gatgtgatcg atgcctaacg gcgccagcgc atccatcacc gcgttggcca
     3961 ccgccggcgg ggcgccgttg gtgccggctt cgcccacccc tttaacgccc agcgggtgaa
     4021 acggattggg tgtgacggtt tcgccaagga tcagccgcgg catatcggtg gcgcgcatca
     4081 cggcataatt catcaatgtg ccggtgagca cctggccgtc ttcgctgtag accatttgct
     4141 cgcgcagggc ttcaccgagg ccttgcgcca cgccgccgtg aatttgccct tcgacgatca
     4201 tcggattgat gagcacgccg caatcatcga caagcacgag cttctcgatc ttgggctcgc
     4261 cggtttcacg gttgatgctg accaaacaaa catgcgcgcc aaagccccat gcctcgcgcc
     4321 tgggatcgaa aaagcaggtt tcctggagac cgttctccat gccttgcgga atttttccgc
     4381 cgtaggctgc cgccgcgatc tggcgccagg tgactttctt ttcggggacg ccggccaccg
     4441 aaaatccgcc gtcagcttga accatgtctt cggcagatgc ttcaaccaga tgggccgcga
     4501 ttcgcttggc cttttcaatc acacgctggg ccgcagtcgc taaagcgccg ccgcccacca
     4561 cagcgctccg gctaccgaag gttccggttc cttgctgcga aacgtaggta tcgccgtgca
     4621 gcacggtgac gtgctccata tcggtcttca acatctccgc ggcaatttgt gcccacaccg
     4681 tttcgtgccc ctggccgtgg gagctcgatc cggtgagcac ggtgatctcg cccgtgcgct
     4741 cgatacgcac cgtgccgctt tcaaagccgg cgccgccact cggctcgaca aacgtagaaa
     4801 cgccaacacc gacgatttcg ccgcgagcgc gccgttcatc gcgctggcgc agcagctctt
     4861 ggtagttggc aattttgatt gcctcgtcga gcgatttgaa ataatcgccg gagtcgtact
     4921 ccacgccgac tgcagtcttg tatggaaact gttccggtcg aataaagttt ttccggcgaa
     4981 tttccagccg atcgatgccg agatccttag cggctttgtc gatcagccgc tcgatgttca
     5041 agaccgactc gggccggccg gcgccacgat atggcccagt tggaacggtg ttggtaaaca
     5101 cgccgacgac ttcgacgtga caatctctga tttgataaca gcccggcgcc atccccatca
     5161 tgcgctgcgg cggcccggcg gtgctcgatt gcagatagcc gccgagattg gcgaccacgc
     5221 gaaccttaag ccccaacatc gtgccgtctt tcttgagcgc caactcggaa gtcatcgcct
     5281 ggtcgcggcc ctgaatcgtc gtgacaaaat catcgctgcg cgtggcgatc catttcaccg
     5341 gacggcgcaa cttcaaagcc atgtaactag tcaagacgtc ttcgcgataa agcggtcctt
     5401 tactgccgaa tccgccgccg acatcgggcg cgatcaagcg gatcttgttt tccgcaatgc
     5461 cgagagtgac ggcgagatcg gcgcgcgccc gatgcgggcc ttgggtggaa agccagactg
     5521 tgaggcgctt gcccatcggg tcgggattgg cggcgacacc gcgcggttca atggccaggg
     5581 caacctggcg cgggctaaca atatgcatgc ggacgatgtg gtctgcttgg gcaaacgctt
     5641 tatcgacgtc gccgcctttc tttactgccg tgtaacagat attgctgtcc aactcttcac
     5701 gggcgagcgg cgcgcccggc ttgagcgcat cttcggcgtt ctgtatcgac ggcagcgctt
     5761 ggtattccac ttcgatggca ttgcacgcat cctgagcaat ggcccggctc tccgccacca
     5821 cggcagcaac cggagttccg ggggcatgca cagcgccgcg cgccagcacc ggatgcggcg
     5881 gaatcttctg acccggaaac atcacttgag cgttgatatt caaacgttcc ggcagttccg
     5941 gcccggttac cacggcgacg acccctttca tcgcttttgc cgcatcggtg ttgatcgaaa
     6001 ttatttttgc atggggatac gcgctgcgca gcaccatcat gtgcaacatc ccgggcaatt
     6061 tgatgtcgtc gacgtaacct cctttgccgc taatcagcag agggtcctct actcgccgaa
     6121 tcgatgcacc gattaaacca ccgccgttgt gttcagttgt cattacttac cctcatagga
     6181 aaataacgtg atcatgttcg tgccttcgtg cctgatagag actaagaagt atcaagtctt
     6241 tcgtcgtcgc gagcacgatc gccggcgcgc agttcttatc ttagatcttg tcttgttctt
     6301 gaacgaacac gatcacgtgc tcacgtctgg tatctttatt tagtttgcgg ctcgttgcac
     6361 cgcggcttct atcgccctgc gcgccagcac cgttgccata tgccttttat actcggcgct
     6421 ggcgtagatg tcgtcatccg ggtcggcgcc ttcggcggct ttagccgccg cggcgacgat
     6481 gttttgtgga ttgaggacgt tgccttgcaa ttccttctcg ctggccaccg cacgaatggg
     6541 accgctgcct aaaccgccga tggcgatgcg cgccgctgcg cacgagccgg attgctggcg
     6601 cgtaatcatg gcgccggcac tgacaactac gtatcccgaa gccggatgcg cgagcttatg
     6661 atacgcgctg ccgctatgga caggcggcaa gggcagcaga atttccgtga gtatttcgcc
     6721 gggcgccatt gccgtggtga aataatcggt aaagaagtcg tccaccggaa ccgttcgatt
     6781 gccgccgatc gaacgcaata caaatgaagc attgagagcg gttaaaatca ccggaaaatc
     6841 cgccgccggg tcggcgtgag cgacactgcc gccgatagtg ccacggttgc gaacctggac
     6901 gtcgccgatc accgacgccg catccaccag tccgggaaaa tatttgccta ctacatcgga
     6961 cgaggcgaca tccgcatgca ccgtcaaagc gccgatcatg agcttatcgc cttcaaggcg
     7021 aacgccggtg agaccgggca cagtccccaa atcgatcaag gtccgcggtg aagccagccg
     7081 cagcttcatc gccggcaaca aactgtggcc gccggccagc aatttggcat cgtcaccatg
     7141 ctgctttaac aactgcagcg cttcgtctag tgactgcggc gcgaggtagc cgaaactagc
     7201 gggaaacatt tttggctccg attttctctg cggcccattt aatcgattcg acgatattca
     7261 cgtaaccggt acagcggcac aggttgcctt cgagcgcgtg cttgatctgt tcctcacttg
     7321 gattggcgtt ggtgcggagc aaatcagcgg cggtcatgat catgccgggc gtgcagtaac
     7381 cgcactgcag tccatgcttt tcgcgaaaac cctcttgcac gggatgaaat tcatcgtcat
     7441 gcgccagccc ctcgatggtg gtaatttttt tgccattggc ctgcaccgcc aaaatcgtgc
     7501 aggattttac cgctcgatcg ttaaggtgga tggtgcaggc gccgcactgg ctggtgtcgc
     7561 aacccacatg cgtgccggtc aaacccagtt ggtcgcgcag aaaataaacc agcaaggttc
     7621 gtggctcgac catggcctca tgcttcaccc cgttgacggt cacggttatc gggagagccg
     7681 cgtactcggc ctcagcagtc atgctcgatc ttcctccaat cgttgattgc atgcatcaga
     7741 attttggaca tcaacttggt ctcttacacc cgattgatgc gggtctcaag atcttggaaa
     7801 ttcggttcaa cgttcaacgg ttcaaaagtt caaggtcaga gttcgacctc cgggggccgc
     7861 accctactag ggaagccgag agcgaaaacc ggagcatcac cttgacagtg gttaaaggta
     7921 ctactatcac gaagacgagg tgctgaaggc ccaggaggca tgatggtcga aatcaaatcc
     7981 ctaatcgatc ccgaaggcgg tcttatcgac cggcgcatct ttgcagatcg agagatttat
     8041 caactggagc gcgaacggat ttttgcgcgc tgctggcttt atctgggcca tgaatgcgag
     8101 atccccaatc ccggcgattt tgttaccgcc tacatgggcg aagagccggt tatcctgtgg
     8161 cgcgacttga accggcaggt tcatgcttat ctgaacgttt gccgccatcg cggcaatcgc
     8221 gtgtgccgcg ccgatcgcgg caacgccaga gtgtttacct gctcctatca cggctggacc
     8281 tacagcagcg aaggcaagct cgcgatcgtg ccgatgccgg aggcgtttcc cggactcgac
     8341 cgcgagcagt ggggtctcat cccggtcgct caactaagcg cctacaaagg tttaatcttc
     8401 gccaccttcg atccgtcggc gccgtcgttg ctcgactact taggtgacat ggcgtggtac
     8461 ctggatattt tgcttgatcg acgtcaaggc ggcaccgaag ttagcggccc gcatcgctgg
     8521 atagtcgatg ccaactggaa aaccgccgcc gagaatttcg gcggcgacgg ttaccacatc
     8581 tcctacacgc atgggtcggc gcgcgaactc ggcgtggata ccacatcgag ctccacccgc
     8641 aagtggaaca aaggctgcca gatcgcctgc ggcaacggtc acattcttgt ttcctggctg
     8701 acgccgcccg atgatgccgg gccttggttc gcgcaaccgg atgaggagtt gctcaattac
     8761 atgaaagaat gcgccggcga gatcgagcag cgattgggca aggtgcgggc gcggcagatc
     8821 tcgccgtcgg ccggcacggt tttcccaaat ttttctgtcc actggcttac ccgtacgatc
     8881 cgtgtgtggc agccgcgcgg tcctgacaaa atggagatct ggagttgggc catcgtcgat
     8941 aaagcagcac cgcccaagat caaggatcga atgcgattcg tttctcaata tcgtttcagc
     9001 cccagcggca tttttgagca ggatgacatg gacaattgga tccaggtcac cggcgccgct
     9061 aaaagcatca tcgccaggcg ctatcccgcc aattaccaga tgagcggcag tgaagaaatc
     9121 attcagcctg agttacctgg gagattggcc agccgattta gcgacaacaa tcaactaaat
     9181 ctgtacatgc attgggccaa aatgatggag gcaaacagct ggaatgaaat cgccggcgcc
     9241 gagcgagcat agacatgaac cacgacctgc gccacgcgat cgaagacttt ttttactttg
     9301 aagcggagtt gctggacgag cgccgcttgc gagattggct cgatctcctg actgacgacg
     9361 ttcaatactg gatgccggtt cgccataatc ctttggaaag atcggcggac ttgaacgacg
     9421 agctatccaa acccggcgac gtctattact tcaacgacac gaaggatacg ctaaaggtta
     9481 gagtagagcg ggtttatgcg aaaaatgcct gggccgagat gccgccatcg cgaacccgtc
     9541 acctggtttc caacgttcgg gttaagaacg acaacggatc ggaaattgaa gttcagtcga
     9601 atttcttgtg ctatcgaacc cgtatggaga aggatcagga tcttttcgtt ggcactcgtc
     9661 acgatttgct gcgcagggcc catggcaact tccggattgc tcgacgaaag atcatcctgg
     9721 atcaagccaa tctggccgca aaaaacatca gcattttcct gtaacactga agttgatcgc
     9781 ctcaccgggg ccttcagctg ccgcggctaa actcaacatc gggaagaacg tttccaagac
     9841 cacagctatg attctaaatc attggtacta cgtcgcggct agattacggg aggtcgttgc
     9901 aaatcggata ttgattgcgc atttcagtgc tataggagtt ctactatgga acgtgaaata
     9961 gaacgcccaa accagatata caagtttgtg atctgcggcg ccaagttcga ttcgcgagag
    10021 gagctggaac aacacgagag caagtgcgct aataatcaac cagcttctta gtggctctcg
    10081 gcagcgggtt tacttcagga ccattgccgc gcccctgagc gcatgaaaca tcggatcgga
    10141 tcagcgattc gatccgcgca cttcgatctg accgtcacgg acgcgaactt ccagcacagg
    10201 ctgcggatgc acggatggtc catttacgac tctgccattc tcgagcgaga aacaggagcc
    10261 gtgccaggga cacttgacta cagtatcctg gagctcgcct tctgcgagtg gcccgccaag
    10321 gtgcgaacat gtttccgcga tggcgtaaat tcgcatgcct cgacgcacaa gcagcaccgg
    10381 cataccatcg gcatcgacgc gccgcagctc gttatcggac aattccgatt cagtgagaac
    10441 cggaaaaaag tcctttggcg gtgaaaaacc cgctgtatga tcgacaccga ttctttcgtt
    10501 ataaaccaga tgcccgccga ggtatgcaga taaattggag atcataaaac ccgccattgc
    10561 gaattggcgt ccagcatttc gtgaatgaac ccggcgcaga cagagcgaag tgacatagca
    10621 agccgttgcc gctaaattca gaactccatg cgcaagaccg atgcgccgtg ctcgactcgg
    10681 gccgactttg gaccagtcgg tcaaaccggt tacagccgcg gctgtcgccc cgatgagacc
    10741 gaaggcaatg gcggcatccg ctttccggcc catcgccggg cttccgcttg tttgttccag
    10801 aaggtcgaac acggcggccg ctgtccagga accgatgggg acatccgtga ggacgacgtg
    10861 cagcggatgt ccgagccaag ttccatgcag gatgtttcca accttcggac cgatagaacc
    10921 gttatcgtcg aacgcagctg caatcgtcgg ttgtagctgc tcctcgatag gttccaacca
    10981 gtgctgcttc tcgatcagtt tgatgggcag gctgctcgac atagtctcct cctgctgatt
    11041 cacagttttc taaacgagga gagcaaggct cgtaccagtg acaagctaac cttgcttaat
    11101 ggtgatagca atcatgtggc agccacgtcg cattcggcct cggccggata cgacggcttc
    11161 ccggtcgcaa gttgatgaag cgcctctgct ggaagccaga atagctcgac agatcgcttc
    11221 aagatttaaa gctggctcat tgtcgtaaga ggaatctttc gtgatcgagg acaagatacc
    11281 tgttcatttt cgcgaattca gcttctcgcg ttgtgctgat ttcaatacaa tctaacgcgc
    11341 caaattggag taagttcttc atcgtcggcg gcaaaatttc gagttcttca ctattcggcg
    11401 gtattctgat cgagtcacac ggtttgccat gatctgcttc caaggatcgg acgacagagc
    11461 cgatctagag atgcaagctg ctttttgtgc cgatcctctt gaaacctgac cacgaattgt
    11521 taatctcgcg aaattacctc ctccccttgt gcatacaaat ccatgcgacg acgcgatttt
    11581 ctcaaatctg ccttgatcct gacgggagca ccgttatcga tcccggcctt gcgcccctcg
    11641 gctgggaggg ggcagcctgt cggcgcagag aatttcaact acgattggct taagagtcac
    11701 gcgcgccgat tatcatccac tcagtaccaa gcacccgacc gagtgctgcc caatacgctg
    11761 agacaacttg actacgacgg ctaccaggcg attcgattcg actccgataa agcgctatgg
    11821 gccagagaga gtctcgcctt tcgcatagag ttttttcatc gtggtccgat gtttaaagag
    11881 ccggtgcgca tgcatgaaat cgtcggtggt caggcaagcg cgatacttta tcatccagcg
    11941 atgttcaatt ttcgcagaag tccagtagat ccgggcgcct tgtcgcaaga tctcggcttt
    12001 gccggtttcc gagtgcattt tcataccgac tggcgcgccg atgtagcagc atttctcggc
    12061 gccagctact tccgcgccgt cggcagtgac tatcggcagt acggaatttc tgctcgcggt
    12121 ctcgccgtgg acaccgccct cgataaaggc gaggagttcc cgcgcttcac cgacttttgg
    12181 ctggaacgac cggtaaaaca ttcgcagcag ttgactatat atgctctgct ggattcgccg
    12241 agcgtgaccg gcgcctatcg cttcgagatt aggcccggga cgaccttgat catggatgtc
    12301 gatgcggcga tttatccgcg caagatgatc gagaggattg gcatcgcacc gctaaccagc
    12361 atgtaccagc acggcgagaa cgatcgacgc atggccaccg actggcgtcc ggaaattcac
    12421 gactccgacg gactgcaact gcttaccggt aatggcgaat ggatctggcg cccattggtg
    12481 aatccatcgg ggattcgagt caactcttat cttgacacta atccgcgcgg cttcggcctg
    12541 ttacagcgcg atcgcaactt cgatcactat caggatgatt ccgtattcta cgagcgccgc
    12601 ccgagtctct gggtcgaacc gaaaatcgat tcgagtggcg gttggggtac cggtgcagtg
    12661 cagctggcgg agctaccagc gccggacgaa actttcgaca acatcgtcgc ctactggact
    12721 cctgccgctc aaccgcagcc cgggcaggag ttgctcttca gctatcggct ctattggggc
    12781 tcgagaatac ccgctgcgcc tgcgctcgcg caagttgtcg ccactcgaac gggtatcggc
    12841 gggatcgttg gtcaaccgag aaaatatttc tcctatcgct tcgctgtgga tttcagcggc
    12901 ggcaagcttg caacgctttc caaagcagcg caagtagagc cggtgatcag cgcttcgcgc
    12961 ggcgaaatcg agattgcatc ggcgcggcct cagcttgaga tcggcggtta ccgcgccatg
    13021 ttcgacttga aacccattga cgataccacc gagcctatcg atctccgctt gtacctgcgc
    13081 cacggcgggc agccgttgac cgagacttgg cagtatcagt ggagaccgcc gcccgtggat
    13141 gagcggaaat tataatgatc tcgcttttgc ctggaacctt cgaccggaac tcatctattt
    13201 ctttttcact ttacgggttg acgatttttt gcgcggcacc ttgccaccgg cgcggcgtgc
    13261 ttcggaaagg ccgatggcga tagcctgttt gcggctcgtc actttttttc cggaccctcc
    13321 gctcttcaat gtgcctttct tgcgctcgtg catcgccttt ttcacttttt gcttcgcctt
    13381 cggaccatac ttcgccatgt ttgctcctcc cgcacaggat ttccgtaact ttgcggcgcg
    13441 tggccggtac ccttcaactt cgaattcgat tactcagcgc agaaaacttt atagttgggt
    13501 ctcctcccag cttattaggt tttgctagac tgacaagtag ggtaggaagc cgatgattta
    13561 gatgctcgtt tccaatcagc ctgatttccg tacaagaacc gctgatctca tcttcgagcc
    13621 aggtgagcta atcgtcgaga attcgtgcac aatggttcgg gctcgctcca caactgaaac
    13681 aggactgtga aactaaccca tgaatgcaac gcaacatatg ctcgcctcag cacgcgcgct
    13741 cggcgccgag cggatgcggc ttctcgggat actaactggc gggcatttcg tgatccattg
    13801 gtttcagcag ttttttcctg tcattctacc ttcgatcaaa tcgggtctgt cattgactaa
    13861 tatagaagtc ggcgctttga cgtcggcgca gcag
//
LOCUS       DASUVS010000409         6010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1494348, whole genome shotgun sequence.
ACCESSION   DASUVS010000409 DASUVS010000000
VERSION     DASUVS010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494348"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VF182_21900"
     CDS             complement(<1..724)
                     /locus_tag="VF182_21900"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX6439786.1"
                     /translation="MDANLKARLFTGFVGIPLLLFLIGWGDPWLFHGFCLLVTFGALH
                     EYFSMGFRGRPGEQIIGIAFGLAVSSIVFFPQLTPVELILAPLLVLMLSLYIFMPGQL
                     EERLARLAWALLGSLYLGYLLPHWSSLFRLPHGRAWVFFVLVVIMAGDTCAYFVGHRF
                     GTQKLAPEISPGKTVEGAIGYVLGSILAGCLMGISLALGLSWWELAILSALLSILGQL
                     GDLFESWIKRVFAVKDSGRLLPG"
     gene            complement(708..1487)
                     /gene="uppS"
                     /locus_tag="VF182_21905"
     CDS             complement(708..1487)
                     /gene="uppS"
                     /locus_tag="VF182_21905"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX6439787.1"
                     /translation="MDLKDLDRSRLPRHVAIIMDGNGRWATLQGKSRIEGHRRGKTSV
                     RVIVEMTRKLGIPFLTLFAFSTENWLRPHDEVHALMGLLETYLIAEQPKMMRYGIRLK
                     AIGNRDRLPLNVRRTLEDVIELTQRNSRMTVVLALSYSGRDEIVRMVNKIRHRVLAGD
                     FGASDLDEEIVAAHTDTPDIPDPDLLIRTSGEMRISNFYLWQIAYTELYVTPTLWPDF
                     REQEYLQALLEYQRRRRRFGRTDEQITEPARLRVGSSGREP"
     gene            complement(1589..2146)
                     /gene="frr"
                     /locus_tag="VF182_21910"
     CDS             complement(1589..2146)
                     /gene="frr"
                     /locus_tag="VF182_21910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX6439788.1"
                     /translation="MNDAIFTQLQKEMEGSVAALRKELGRVRTGRASTALLEGVMVDY
                     YGATTPLNQLATLSAPEPRLLVIQPFDRNALGEIEKAILKSDLGLTPANDGKIIRVPI
                     PQLTEERRKDLVKHVKKTGEEFRVSVRNHRRLAIEHLKESEKKKEITADEFKHGQDRV
                     QKITDDYIARVDKVLKTKEEEIMEV"
     gene            complement(2143..2874)
                     /gene="pyrH"
                     /locus_tag="VF182_21915"
     CDS             complement(2143..2874)
                     /gene="pyrH"
                     /locus_tag="VF182_21915"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX6439789.1"
                     /translation="MSEANLKYKRIILKVTGEIFAGAQKFGIDGQTVKAFAQEIKEVK
                     EIGCEMALVIGGGNIFRGAVASDIGMDRASADTMGMLATVINSLALQDALEKLGVSTR
                     VLSAIEMRQVAEPYIRRRATRHLEKGRVVIFAGGTGNPYFTTDTTASLRAMEIGAEVI
                     LKATKVEGVYDADPFKFEGATKFEELTYIEVLNRELKVMDSTAISLCMDHQLPIVVFN
                     LMKKGNIKRVVAGEAIGTLVSAGKR"
     gene            complement(2894..3502)
                     /gene="tsf"
                     /locus_tag="VF182_21920"
     CDS             complement(2894..3502)
                     /gene="tsf"
                     /locus_tag="VF182_21920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX6439790.1"
                     /translation="MEVSAAAVKDLREKTGAGMMDCKKALAECGGDLNKAVDYLRQKG
                     LAAATKKASRTASDGAIGAYVHPGGKIGVLVELNCETDFVARTAEFQNLLKDIAMQIA
                     AANPRYIRPEEVPSGHLEKEREIYRRQALESGKPEKVVDKIVDGKIDRFYSEVCLLEQ
                     SFIKNPDQKVADILTEAIARLGENIQVRRFSRYQLGEGALQE"
     gene            complement(3506..4297)
                     /gene="rpsB"
                     /locus_tag="VF182_21925"
     CDS             complement(3506..4297)
                     /gene="rpsB"
                     /locus_tag="VF182_21925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX6439791.1"
                     /translation="MTEISMKQLLEAGVHFGHQTSRWNPKMKPYIFGARNGIHIIDLQ
                     QTVTMFKEVEAVVRDIAASGGSILFVGTKKQAQDSVREEAERCGMFHVHNRWLGGTLT
                     NFSTIRQSIDRLRKLEEMESDPKIVEALTKKEMLGLKREKEKLEQSLGGIKAMRKLPD
                     AIFVVDPKQEEIAVKEARKLGIAVIAVIDTNCDPDMVDHKIPGNDDAIRAIRLFCSSM
                     ADAVIEGKGLYDQALTKDKGDDGSAAVPAATEMDQPIEQSAAGGV"
     gene            complement(4527..5774)
                     /gene="rho"
                     /locus_tag="VF182_21930"
     CDS             complement(4527..5774)
                     /gene="rho"
                     /locus_tag="VF182_21930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX6439792.1"
                     /translation="MNLKALKEKKISELALIGKNFNIEGAANMRKQELIFAILQAQTE
                     QNGHIYGEGVLETLPDGFGFLRAPDYNYLPGPDDIYVSPSQIRRFSLRTGDIISGHIR
                     PPKEGERYFALLKVESINYEDPERAREKILFDNLTPLYPNERLRLEYQPEDYTTRIVD
                     LLTPIGKGQRGLIVAAPRTGKTMMLQHIAKAIANNHKEVVLIVLLIDERPEEVTDMQR
                     SVDGEVISSTFDEPATRHVQVAEMVIEKAKRLVEHGKDVVILLDSITRLARAYNTVVP
                     PSGKILSGGVDSNALHKPKKFFGAARNIEDGGSLTIMATALIDTGSRMDEVIFEEFKG
                     TGNMEVHLDRRLMDKRVFPAIDINKSGTRKEELLISKEDLNRIWILRKVLSQLSVVDA
                     MEFLLDKMHGTKDNKEFFESMNQ"
BASE COUNT         1411 a         1691 c         1384 g         1524 t
ORIGIN      
        1 gacccggaag gagcctcccc gaatccttaa cagcaaaaac ccgtttgatc cacgattcga
       61 acaagtcgcc gagttggccc aaaatgctta gcagcgccga caagatggcg agctcccacc
      121 aactaaggcc cagggcgagg gagatcccca tcaaacagcc cgccaaaata cttccgagta
      181 catatccaat cgctccctca acggtctttc cagggctgat ttcgggggcc aacttctgcg
      241 ttccgaagcg gtgcccgacg aagtaggcgc acgtgtcgcc tgccataatc accacgagaa
      301 caaagaacac ccaagctctt ccatgtggca gtcgaaagag cgaactccaa tgcggcaaaa
      361 gatagcccag gtacagtgaa cctaagagag cccacgcgag tcgcgcgaga cgctcctcga
      421 gttgcccggg catgaaaatg taaagcgaca acatcaggac aagcaatgga gctagaatca
      481 gctctaccgg cgttagctgg ggaaagaaga cgattgagga aacggcaaga ccgaaagcta
      541 tgccgattat ttgttctcca ggacggccac ggaaacccat cgaaaaatat tcatgcaacg
      601 cgccaaacgt aaccagtaaa caaaagccat gaaacaacca agggtcgccc caaccaatta
      661 agaaaagtaa aagcggaatt ccgacaaatc cggtaaacag ccttgcctta aggttcgcgt
      721 ccactactcc ccactctcag acgcgccggt tcggtaatct gctcatccgt tctcccgaac
      781 cgcctgcgac ggcgttgata ctcgagcaga gcttgcaggt attcttgttc acgaaagtca
      841 ggccaaagag tcggagttac atagagttcg gtataggcaa tctgccataa gtaaaagttg
      901 ctgattcgca tttcaccgct ggtccgtatc aagagatcgg gatcgggtat gtcgggtgtg
      961 tccgtatgag ctgcaacgat ttcctcatca agatcgctgg caccgaagtc gcctgcaaga
     1021 actctatgtc taatcttatt gaccattcgc acgatctcat cgcgtccact ataactcagc
     1081 gctaaaacca cggtcattcg actgttgcgc tgggttaact ctatcacatc ctccagagtc
     1141 ctgcggacat ttagcggcag tcgatctcgg tttccaatcg ctttcaacct gatgccgtaa
     1201 cgcatcattt tgggttgctc cgcaatcaga taagtctcga gcaagcccat cagcgcatga
     1261 acttcatcgt gcgggcgtag ccagttttcc gtggagaatg caaagagagt caaaaaaggt
     1321 attcccagct ttcgcgtcat ttcgacaata acgcgcactg aagttttgcc acggcggtgt
     1381 ccttcgatac gactttttcc ctgcagagtc gcccagcgcc cgttcccgtc catgatgatc
     1441 gctacatggc gtggcagccg acttctgtcc aggtccttaa gatccattgt tgagccgtgc
     1501 aggtgcgtga ggtggtgaga ctatggcgcc ccaaatttga gcggaatgcg gcacaagtga
     1561 ggttggttgt taagcggaga tccggcccct atacctccat gatctcctcc tctttggttt
     1621 ttaaaacctt gtcgacgcga gcgatatagt catcggtgat cttttgaacc cggtcctgac
     1681 cgtgtttgaa ttcatcggcg gtaatttctt tctttttttc cgattctttt aaatgctcga
     1741 tggccaagcg ccggtgatta cgcactgaga cgcgaaactc ttcgccagtt ttcttgacgt
     1801 gctttaccag atccttacgg cgctcttccg tcagttgggg tatggggacc cgaatgatct
     1861 tgccatcgtt agcgggagtc agtcccaagt cgctcttgag aatcgccttt tcaatttcac
     1921 cgagtgcgtt tcggtcaaaa ggctgaatca ccaagagcct tggctccggt gcgcttaatg
     1981 tagccaattg attcaaagga gtagtggcgc cgtaatagtc aaccataacg ccttccaaaa
     2041 gagccgtcga tgctctcccc gtccgaactc ttcccaactc tttgcgcaga gcggcaaccg
     2101 acccttccat ctctttttgc aattgagtga agatagcgtc gttcatcgtt tccccgcact
     2161 tactaatgtg ccgattgctt caccggcaac gactcttttt atgtttccct tcttcatcaa
     2221 attaaaaacg acaatcggaa gctgatgatc catgcaaagc gagattgccg tcgaatccat
     2281 aacctttaac tcacggttta aaacctcgat gtaagtcaat tcttcaaatt tcgttgctcc
     2341 ctcaaatttg aacgggtcag cgtcatagac gccttccacc ttggtggcct tcaatatgac
     2401 ttcggcgcca atctccatcg ctcgcaggct cgccgtagtg tcggttgtaa aatacggatt
     2461 gccggtgccg ccggcgaaaa tgaccacgcg ccctttttcc aaatgccgtg ttgccctacg
     2521 cctaatgtaa ggctctgcaa cctgccgcat ttcaatcgct gataacactc gcgtagaaac
     2581 acccaatttc tccaaagcgt cttgtagcgc aaggctgttg ataacagtgg ccagcatgcc
     2641 catggtatcg gcgctcgccc tatccatgcc aatgtcgctc gcgactgcgc cacgaaaaat
     2701 attgccgccg ccgatgacca acgccatctc gcagccgatc tctttgactt ctttgatctc
     2761 ctgggcaaac gccttgaccg tttgcccatc gattccaaac ttctgtgcgc cggcgaagat
     2821 ttcgccggta actttcagaa tgatgcgttt gtacttgaga ttcgcctcgc tcatgcgctt
     2881 tgtccgacga tccttactcc tgcaatgctc cttcgcccaa ttggtatctc gagaaacgcc
     2941 taacctgaat gttctcgcca agacgggcta tcgcctccgt gaggatgtcg gcaacttttt
     3001 ggtcggggtt cttgataaac gattgctcaa gcagacaaac ttctgaataa aaccgatcga
     3061 tcttgccatc gacgatcttg tcgacgacct tttccggctt acctgactcc aaagcctgcc
     3121 ggcgatatat ctccctttct ttctccagat ggccgctcgg aacctcctcc ggccgtatgt
     3181 atcgcgggtt agcagcggca atttgcattg cgatatcttt taaaaggttc tgaaactcag
     3241 cagtgcgcgc gacaaaatcc gtttcgcagt tgagctccac cagcacgcca attttgccgc
     3301 ctggatgcac gtatgcgccg atggcaccat cactggcggt tcgggatgct tttttggtgg
     3361 ccgctgccag ccccttctga cgcaaataat cgaccgcttt gttgagatcg ccgccgcatt
     3421 ctgccaacgc cttcttgcag tccatcatac cggcacccgt tttttcacga agatctttta
     3481 ccgcagcggc actcacttcc atggactaaa ctcctcccgc agccgactgc tcaattggct
     3541 gatccatctc ggttgcggca ggaacggcag cagatccatc gtctccctta tctttggtga
     3601 gagcttgatc ataaagcccc ttaccctcta tcactgcatc ggccatggat gagcaaaata
     3661 agcgaatggc tctgatagca tcatcatttc ccgggatctt gtgatctacc atgtccggat
     3721 cacagttcgt gtcgatcacg gcgatgacag caataccaag ctttcttgcc tcctttacgg
     3781 caatctcttc ctgcttggga tcaacaacaa aaatcgcatc ggggagctta cgcatcgcct
     3841 tgattccccc aagcgactgc tcaagctttt ccttctcccg ctttaggccc agcatctcct
     3901 ttttggtcaa agcctcgacg atcttcgggt cactttccat ctcctcgagt tttctcaagc
     3961 ggtctatact ctggcgaata gtcgagaaat tcgtgagagt gccgcctaac cagcggttgt
     4021 gaacatggaa catcccgcat cgctcagcct cttccctaac cgaatcttga gcttgttttt
     4081 tggtcccgac gaaaagaatg cttccgccgg aagccgcaat atcacgcacg accgcttcta
     4141 cttctttaaa cattgtcaca gtctgttgta aatcgatgat atgaattccg ttccgtgctc
     4201 cgaaaatgta cggcttcatc ttcggattcc agcggctggt ttgatgtccg aaatggacac
     4261 ccgcctctag aagttgcttc attgagatct cggtcatata tctgacgctc ctttggttat
     4321 gcctcccctt ctctcgactc ctcctcgttc cctttccggg caccacaaag aggatcaaaa
     4381 agggtgtgaa ttaataagaa aacgccttcg gcatccggcc ggaccggtca ccgaaggctg
     4441 ggaacactgc tgattgacaa ttggtaacct tgcgtttttc tagcaaagaa cagccaaaga
     4501 cgcaaggaaa gtaaagcggg caaatgctat tggttcatgg attcaaaaaa ttctttattg
     4561 tctttggtac catgcatctt atccaacaag aattccattg catcgaccac actcagttgg
     4621 gacagaactt tccgcaaaat ccagattcgg ttgaggtctt ctttggaaat cagcaactct
     4681 tcctttctgg ttccagactt gttgatgtca atcgcaggaa aaaccctctt gtccatcaag
     4741 cgacggtcta ggtggacttc catgttgcca gtacctttaa attcctcaaa gatcacctca
     4801 tccatccggc taccggtatc gatcagcgca gtcgccataa tcgttagact gccgccatct
     4861 tcaatgttgc gcgcggcgcc gaagaatttt tttggtttat gcagggcatt ggaatcgaca
     4921 ccgccggaca aaatcttgcc gctgggaggg acgaccgtat tgtaagcgcg cgccaagcgg
     4981 gtgatgctgt cgagtagaat cacaacatct ttcccatgtt ccaccagacg ctttgccttt
     5041 tcgatgacca tttcggcaac ctgaacgtgc cgcgtggctg gttcatcgaa tgtggaacta
     5101 atgacctctc cgtcgaccga acgttgcatg tccgtgacct cctcgggtcg ctcatcgatc
     5161 aagaggacaa tcagcactac ttctttgtgg ttgttggcga tggctttggc gatgtgctgc
     5221 aacatcattg ttttaccagt ccgtggtgcg gcaacgatca gtccacgttg acctttgcca
     5281 atgggagtca acaggtcaac aattcgcgtt gtgtaatcct caggctgata ctcgagtcgc
     5341 aagcgttcgt taggataaag aggtgttagg ttgtcaaaaa gtattttctc gcgggctctc
     5401 tcaggatcct catagttaat cgactcgact ttaagcaagg caaaatatct ctcgccctct
     5461 ttcggtggcc tgatgtggcc ggagataatg tcacccgtac gcagactgaa ccggcgtatc
     5521 tggctgggag agacataaat atcatccggg ccgggcagat aattgtagtc aggggcgcgc
     5581 aagaacccga agccatcggg aagagtctcc agaacgcctt ccccgtaaat atgaccattc
     5641 tgctccgtct gcgcctgcag gattgcaaag ataagctcct gcttgcgcat gttggcagcg
     5701 ccctcaatgt tgaagttttt accgatcaac gcaagttcgc tgatcttctt ttcttttagc
     5761 gccttcaaat tcagcccatt gtcttccgca gcctcagact caacggcgcg agttctacca
     5821 cgaaccataa ctatgcctct cctttcatct ccaaaccaag agcggatacc acccctctgc
     5881 atactgaaag ttcacgtgca acagcttaca aactgaattc ccggatctat gcacctgccg
     5941 ggagagttat agaggtgaac acgattataa atgcagcctg tccctatggc aggcttcttg
     6001 tgcgcagcag
//
LOCUS       DASUVS010000410         7079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_838971, whole genome shotgun sequence.
ACCESSION   DASUVS010000410 DASUVS010000000
VERSION     DASUVS010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838971"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1855
                     /locus_tag="VF182_21935"
     CDS             200..1855
                     /locus_tag="VF182_21935"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6439793.1"
                     /translation="MANLITPQADLAFVNGKIVTVNSKDEITEALAVRGTKILRVGPR
                     ERVDPTIGPDTKVIDLQGRSLLPGFIENHIHMTNSPQRYWVDCSYATCPSIGDIREKI
                     AARARETKNGDWILGRGFQSARLSERRNPNRFDLDAVSPNNPVGIANREGMGWTFNTL
                     GLRRIGVQDDTPDPPGGPMERDPEGRPLGPMWDNTREVFIKPNLPPYTLDDLINGYRW
                     ISAELNRSGITTAFEAAIRNRIETVAWRKLLQETAPTLRVVMGPYPVNGEEWSSNGTA
                     GKIYDTGFATGFGDAWMKLGALQIGIDGGVIGQTAALFEPYSNDPKQAWRGSFRMSQA
                     VANAFMLQAQKNDWQVGLIAHGDHGIARALEAIDHARKKAPGGDLRHRLEHAYLWNPK
                     VMDRMGDLGVIWNTQPVLLEITGREGIYGQWGDRARFAFPFKSLTERGVIISGGSDWP
                     VGVYNPLIGIDILVNHRFGPEENGEVLNQDERLSVLQAIRVYTYNGAYCAFEEDEKGS
                     LEEGKLADVSVLSDDILKVAPGKIRDLKVDLTYVDGQLVYKRT"
     gene            2055..3080
                     /locus_tag="VF182_21940"
     CDS             2055..3080
                     /locus_tag="VF182_21940"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6439794.1"
                     /translation="MGLVKKIGFGPVIFFVLLSISSAWAQPDHTAKLIEGAKKENRLL
                     WYTALTLADAGMLTQHFEQKYPFIKTETLRLGTDQLLTKIQTEARAGAFRADVIEIPG
                     ILGQVLKQDGLLEKYISPESNAYPASMKDPDGTWTSFFINTHVTVYNTNLVKKQELPK
                     TFEGLIDPRWKGKIALRDNDFDNFGMMLRVMGREKGLNFMRRLAAQGVDLRSSFAGAV
                     QGISSGEIAIGMNLYATHTEEFKKKNAPVDWTPMEFVLASIEPLGIGARAPNPNAARL
                     FVDFLLSKEAQTMMRGRSRIPSRPDVPPDPPELTRGLNIIATDVSLANESKQLAKEFY
                     DIFKSGK"
     gene            3396..4703
                     /locus_tag="VF182_21945"
     CDS             3396..4703
                     /locus_tag="VF182_21945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEX6439795.1"
                     /translation="MTKKETAEVLSIEGREVRVTNPDKAYFSKQTKLSKLDLVRYYLS
                     VAPGALAGIRDRPVVLKRFVDGAEGEAFYQKRAPANRPSWLRTVTLSFPSGRKAEEIV
                     VDDAAGLAWIVNLGCIELHPHPVRSGNLEHPDELRVDLDPVPGVEWEDVRRVALEVKS
                     FLEEMGLCGWPKTSGSRGMHVNVRIETRWTFLEVRRAALALSRAVERRLPAMASSKWW
                     KEERHGVFLDYNQNAKDRTTCSAYSVRPVPDARVSMPLHWHEVAECNPADFTVLTAPA
                     RFAEFGDPHAAMDASAGSLEKLLDLAAQDEAAGLGDAPWPPHFRKMEGEAPRVQPSRA
                     RSAAKKTSAKKPRPRAPLLVIANSSSREAALAGLERWKAKHPEVAALLAPDDVLVDSM
                     RGRSSTWTRIRVNLRNVPEALRPAQETPDPDDDPTRAWRTRRQ"
     gene            complement(4847..5935)
                     /locus_tag="VF182_21950"
     CDS             complement(4847..5935)
                     /locus_tag="VF182_21950"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX6439796.1"
                     /translation="MRLPVNPPVLPMLAKRVSELPRGANWIFEPKWDGFRALVFRDGE
                     EVLIQSRDEKSLNRYFPELLEPLRLQLPARCVLDGEIVIAKDSALDFDALQLRIHPAA
                     SRVKLLSTEIPASIVLFDVLSEGDRDLRGMPFRDRRRILESLFSSASPPLHLTPVTSE
                     LGRAQDWFRRFEGAGLDGVIAKPVEGAYEPNKRVMLKIKHERECDCVVAGFRWHKKGD
                     RAAVGSLLLGLFNESGTLQHVGVCASFTDKKRRELVEFLAPYRKNALTAHPWKDWADH
                     APEGTDPERRMPGGQSRWSGGKDLSWEPLRPELVVEVAYDHMQGNRFRHTAQFRRWRM
                     DKKPSDCTYDQLEIVAPEELAVIFADSR"
     gene            complement(6154..7029)
                     /locus_tag="VF182_21955"
     CDS             complement(6154..7029)
                     /locus_tag="VF182_21955"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6439797.1"
                     /translation="MRIAIGLPSRVSSASGAVMLEWITRAERGPFSSVVVTDRVVSQA
                     LEPLTVLAMAAGATQRVRVMTSVVIGPTRETTLLARQAATIDLLSGGRLTLGIGIGVR
                     KNDYLATGFDFHRRGRRAEEQLPMLRRLWSGEAMSNETGAIGPRPYRANGPELLIGGY
                     VPAIVQRIAKWGDGYMAPGGGEPENLLKTWRQIENAWQQAGRAGKPRWVGASYYALGP
                     NARDHASRYINANYGYNPDLAARRLSTLPASVAAVEEAIKRQGDMGVDEFILRPCADE
                     LDQMDRLAEIAARTT"
BASE COUNT         1551 a         2003 c         2029 g         1496 t
ORIGIN      
        1 cgataggttg ttgttaatcg atcgaacaag cctgaatgac tcgcacgatg agccgctgat
       61 gagtcgattg actctcgctg aactaccaca ttgacgcgta atttccttga caagcaaacc
      121 gcagaggagc tagcggtgga caagcgatgt ctctcgagca tgtacagacc tatcggttct
      181 gtatttcgaa ggtcaatcaa tggctaactt gattacaccc caagcggatt tggcgttcgt
      241 gaacggcaag atcgtcaccg ttaattcgaa ggacgaaatc actgaggctc tcgcggtccg
      301 cggcacaaaa attctccgcg tcggcccccg cgagcgtgta gatccaacca ttggtcccga
      361 taccaaagtc atcgatctcc aaggccgatc gctattgcct ggattcatcg agaaccatat
      421 ccatatgacc aattcacccc agcgctactg ggtcgattgc agttatgcga cctgtccatc
      481 gattggcgat atcagggaga aaatcgccgc gcgggcgcga gagacgaaaa acggcgactg
      541 gatcttggga cgaggttttc agtcggcgcg tctatcggag cggcgtaatc ccaaccgttt
      601 cgatcttgat gcggtttcgc cgaacaaccc ggtcggcatc gccaatcggg aaggcatggg
      661 ttggactttc aacacattgg gccttcgacg catcggtgta caggatgata ctccagatcc
      721 tcccggcgga cccatggagc gcgacccgga agggcgaccc ctcggaccga tgtgggataa
      781 cactcgtgaa gtgtttatca aaccgaactt gccgccgtat acactcgatg acttgatcaa
      841 cggctaccgc tggatcagcg cggagttgaa ccgtagcggt atcaccactg ctttcgaggc
      901 ggcgattcgc aatcggattg aaaccgttgc ctggcgcaag ctgctgcaag aaactgcgcc
      961 gacgctgcgg gttgtcatgg gcccttatcc agtcaacggc gaggagtgga gttctaatgg
     1021 tactgccggc aaaatttacg ataccggttt tgccaccggc ttcggcgacg cttggatgaa
     1081 acttggcgcc ttgcagatcg gcatcgatgg cggcgttatc ggtcagactg cggctctttt
     1141 cgaaccctac agcaacgatc ctaagcaagc gtggcgcggc agttttcgaa tgagccaagc
     1201 agttgccaat gcatttatgc tgcaggcaca aaaaaacgac tggcaggtgg gtctgatcgc
     1261 gcacggcgat catggtatcg cgcgcgcgct cgaggcgatc gatcacgcgc gaaaaaaggc
     1321 gcccggcgga gacctgcgcc accggctcga gcatgcttat ctgtggaatc ccaaggtgat
     1381 ggatcgcatg ggcgatctcg gggtgatttg gaatacccag ccggtattgt tggagattac
     1441 cggccgcgag ggtatttacg gacaatgggg cgatcgcgcg cgctttgcct ttccgttcaa
     1501 atctctgacc gaacggggcg tgatcatctc gggaggctcc gactggcccg tcggcgttta
     1561 caatcccttg atcggtatcg acatcctggt caatcaccgc ttcggtcccg aagaaaacgg
     1621 cgaggtgttg aatcaagatg aacggttgag cgtgcttcaa gcgattcgcg tctacactta
     1681 caacggagcc tactgcgctt tcgaggaaga tgaaaagggc tccctggagg agggaaaact
     1741 tgccgacgtt agcgtgttgt cggacgatat tcttaaggtt gcgccgggga aaatacgcga
     1801 tctcaaagta gatttgacct acgtcgacgg acaactcgtt tataagcgaa cctgagaacc
     1861 cagctaagtg acgcaacgtt tggacggcta tcttagttaa aggaccacgc acgccgctaa
     1921 aaataactgc ggtgcaatcg aaattgcctg aacgtcattc tgagcaaggc gaagcatttc
     1981 gctccgttac tgacgccaaa ttctttgttt ccgcctcacg acgacagcgc aaaaacgctg
     2041 taagggagga gcccatggga cttgtcaaga aaataggctt tggcccagtg atctttttcg
     2101 tactcttgtc aatttcatcc gcctgggccc aacctgatca cacggccaag cttatcgagg
     2161 gcgcgaagaa agaaaacagg ctgctctggt acacggcgtt aactcttgcc gacgccggca
     2221 tgttgaccca gcacttcgag caaaaatacc cgttcatcaa aaccgaaacg ctccggctcg
     2281 gaaccgatca gttgctgacg aaaattcaga ctgaagcgcg cgcgggagcg tttcgggccg
     2341 atgtgatcga gattcccggt atcctggggc aggtgctcaa acaagacggg ctgttggaaa
     2401 aatatatatc tcccgaatcg aatgcctatc cggcttcgat gaaagaccct gacggtactt
     2461 ggacaagctt tttcatcaac acccatgtca cagtttacaa caccaacttg gtcaagaaac
     2521 aggagctgcc aaagaccttt gaaggtctta tcgatccgcg ctggaaaggc aagattgcgc
     2581 ttagggataa tgacttcgac aacttcggta tgatgctgag agttatgggc agagagaaag
     2641 gactcaattt catgcgccgg ctggccgcgc aaggagtgga tttgcgcagc agctttgccg
     2701 gcgccgttca gggcatttct tccggagaga tcgccatcgg catgaatctc tatgccactc
     2761 acacagaaga gtttaagaaa aaaaatgccc cggtggattg gactccgatg gaatttgtcc
     2821 tggcctctat cgagcctttg ggaatcggcg cgcgcgctcc caatcccaat gccgctcgcc
     2881 tgtttgtcga ttttctgctc tcaaaagagg cgcaaaccat gatgcgcggg cgcagccgca
     2941 ttccctcacg ccccgatgtg ccacctgatc ctcccgagct cacccgcggc ttgaatatca
     3001 ttgctaccga tgtttcgctg gcgaacgaat caaaacagct ggcgaaggag ttctacgaca
     3061 ttttcaaatc cggaaaataa ccccactatc gagcatgaga cggcctgaac gccttgactt
     3121 tgagtgactt gccgtactga tccgacgtat tgatcggcga tagccgcact tgatagtagg
     3181 acaagccacg atttcttttg ctgatttcag gagatcgaat ctctcgcgca gcatgcatcg
     3241 taatctcgga ctcggtttcg gcggctagag gcaactacta agcgtgtcga tttgtgccgg
     3301 cggccggctc cggtagagat gaagcagtag actgacccgc tggaactgtt aagctttatg
     3361 cgcgtgagca atccggcagg cggttagttc attccgtgac aaaaaaagag acagcggagg
     3421 ttttgtcaat cgagggacgt gaggttcggg tgaccaatcc cgacaaagcg tatttctcga
     3481 agcagaccaa actctcgaag ctcgatctcg tgcgctacta tttgtcggtg gcgccgggcg
     3541 cgcttgctgg catccgcgat cgccctgtgg ttctcaagag attcgtcgac ggagccgaag
     3601 gggaggcgtt ctatcagaag cgcgcccccg ccaatcggcc gtcgtggttg cgaaccgtaa
     3661 cgctgtcgtt tccttccggg cgcaaagcgg aggaaatcgt cgtcgatgac gcggccggac
     3721 tcgcgtggat tgtgaatctg ggctgcatcg agcttcatcc acatccggtg cgctccggca
     3781 atctcgaaca tccggacgaa ctgcgcgtcg atctcgatcc agttcctgga gtcgaatggg
     3841 aggatgtccg ccgtgttgct ctcgaggtga aatcgtttct cgaggagatg gggctttgcg
     3901 gttggccgaa gaccagcggt tcacggggta tgcacgtcaa tgttcgtatc gaaacgcgct
     3961 ggaccttcct tgaggtgcgc cgcgccgcgc tggcgttgtc acgcgccgtt gagcgtcgtc
     4021 tgccggccat ggcaagttcc aaatggtgga aggaggaacg tcatggggtt ttcctcgact
     4081 acaaccagaa tgccaaggac cgcacgactt gctctgcgta ttccgtacgg cccgtgcccg
     4141 acgctcgggt ttcgatgccg ctccactggc atgaggtcgc cgaatgcaat ccagccgatt
     4201 tcactgtgct cacggccccg gcgcgcttcg cggagttcgg cgatccgcac gcggccatgg
     4261 acgcctcggc aggttcgcta gagaagctgc tcgatctcgc ggcacaggat gaggcggcag
     4321 ggctgggcga cgcaccctgg ccgccacact ttcgtaagat ggagggcgaa gcgccgcgtg
     4381 tgcagccttc gcgcgcccgc tcggcggcaa agaaaacaag cgccaagaag ccacggccaa
     4441 gagcgccgct tctggtgatc gcgaactctt caagcagaga agccgcgctc gccggcctgg
     4501 aaagatggaa ggctaaacat cccgaagtag ccgcgcttct cgcgccggat gacgtgctgg
     4561 tcgattcgat gcgcggccgg tcgtccacct ggacgcgcat ccgagtgaac ctgcgaaatg
     4621 tgccggaagc attgcgcccc gcgcaagaaa ctcccgatcc tgacgatgat cccacccgtg
     4681 catggcgtac ccggcggcaa taagggttca agtcgttcaa ccagttcaaa tcgttcaatc
     4741 ccatcttcat cttccccgcg gtgcacggga aaggaactcc gaaaaaacag tttgaaggga
     4801 gcgtagcggt tgaacggctt gaacgttttg aaccgatact gtggcgtcag cggctgtcgg
     4861 cgaagatcac cgccagctct tctggtgcga cgatttcgag ctggtcataa gtgcagtcgc
     4921 tcggcttctt gtccatgcgc cacctgcgaa actgggccgt gtgacggaag cggttgccct
     4981 gcatgtgatc gtaagcgact tcgacaacca gctcgggacg gagcggctcc caagacaaat
     5041 ccttgccgcc gctccagcgg ctctggccgc ccggcatgcg acgctcggga tccgtgccct
     5101 cgggtgcatg atccgcccaa tctttccagg ggtgagcagt taatgcgttc ttgcggtatg
     5161 gcgcgaggaa ctctaccaac tcgcggcgct tcttatctgt gaagctcgca cagacgccga
     5221 catgctgaag tgtgccagat tcgttgaaaa gaccgagcaa aagcgagccg acagcggccc
     5281 ggtccccttt tttgtgccag cgaaaaccgg caaccacaca atcgcattca cgctcgtgct
     5341 tgatcttgag catcacgcgc ttattaggtt cgtacgctcc ctcaacaggc ttcgctatga
     5401 cgccgtcgag accggcgccc tcgaagcgac gaaaccagtc ctgcgcccgg cctaattcgc
     5461 tggtcaccgg ggttaagtgc agtggcggcg atgcagatga aaacagcgac tccagaattc
     5521 gccgacgatc tctaaacggc atgccgcgca aatcccggtc tccctccgaa agaacgtcga
     5581 ataacacgat ggaggccggg atctcggttg aaagaagctt tacccgcgag gctgcgggat
     5641 gaatacgcag ctgcaaggca tcgaagtcga gcgcactgtc cttcgctatg acgatttcac
     5701 catcaagcac acaacgagcc ggcaactgta aacggagcgg ctcgagcaat tccggaaagt
     5761 accggtttag tgatttttca tcgcggctct ggatcagaac ctcttcgcca tcgcgaaaca
     5821 ccaaagcgcg gaatccatcc cattttggtt cgaagatcca atttgcgccg cggggtaatt
     5881 cgctgacccg tttggcgagc atcggcaaaa ctggtgggtt cacaggaaga cgcatactta
     5941 ctctcctgag cgttcaaatt gtccaagccg ttcaatcgct acgctcggtt catgtcgttc
     6001 aaatagcgat tgggttccaa tcgttccagg tgttcaaata agcgaatggg ctagagtcgt
     6061 tgtactcgtt ccaaccgttg catcgctgcg ctccgaatca attattggaa tgcgcgagct
     6121 ggccgggcgt tcgagttcag gacgctggct gccttaagtg gtgcgcgcgg caatttcggc
     6181 gagccggtcc atctgatcca actcgtcggc gcatggccgc agaatgaatt catcgactcc
     6241 catgtctccc tggcgcttga tcgcctcttc tacagccgca accgaagccg gcaaggtact
     6301 taggcgccgc gccgcaaggt cgggattgta gccgtagttc gcgttgatgt agcggctcgc
     6361 atgatcccga gcgttcgggc cgagcgcgta atagctcgcg cccacccaac gcggctttcc
     6421 ggcgcgtccg gcttgttgcc atgcattctc tatctggcgc catgtcttga gcagattttc
     6481 cggctcaccg ccgccgggtg ccatgtaacc gtcgccccac ttggcgatgc gctgcacaat
     6541 ggctggaaca taaccgccga tcagtagttc gggaccattg gcgcgatagg gccgcggccc
     6601 gatggcgccg gtttcatttg acattgcctc gcccgaccac aaccggcgca gcatcggcaa
     6661 ctgctcttcg gcgcggcgac cgcggcgatg aaagtcgaag ccggtggcaa gataatcgtt
     6721 tttgcgcacg ccgataccga tgccgagagt gagtcggccg cctgatagca gatcgatggt
     6781 tgcggcctga cgggccagga gcgtggtttc gcgggtcggc ccaatcacga cgctggtcat
     6841 cactcgaacg cgctgcgtcg ctccggcggc catcgccagg acggtaagcg gctcgagtgc
     6901 ttgggaaacc acccgatcgg ttaccacgac actcgaaaag ggccctcgtt ccgcgcgggt
     6961 gatccattcg agcatcaccg cgccggaagc ggaggatact cgactcggta agcctatggc
     7021 gatgcgcata ctttcctcca tgctctgatt tgaaggtcag gttacaagaa ttttctctc
//
LOCUS       DASUVS010000411        19121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_402738, whole genome shotgun sequence.
ACCESSION   DASUVS010000411 DASUVS010000000
VERSION     DASUVS010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 19121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19121
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402738"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            780..4145
                     /locus_tag="VF182_21960"
     CDS             780..4145
                     /locus_tag="VF182_21960"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6439798.1"
                     /translation="METESEFRVLFEAIPGLVVALRPDPPAFTIIAATNGYLKATGTR
                     RKDIVGGGFFDVFPGCRGEARNNATANLKRSFETVLRNRSADIMAIQKYPICPVELSG
                     EGWEERYWRGINAPVLGPSGEVAYILHRAQDMTELVRLKEQEKEHEQLKHELQLRAEK
                     AEAKQLAREQQIQRLNRHLRRALTRQQKSRTEAYLPKDWLEHVLLEVHVQVFALDRKW
                     RYVYVNERVTQVVGLTKAQMLGKTIWELFPEVVGSVFERQIRQAAAEFSPVHFEYCYP
                     PWNRWFEMHVYPSAERLTFFVAEITDRKKAEAALQESEERLAGELVTMNRLHELTLRL
                     FSAVDLRAAMDEVLEAVTALMGTNMGDIRIYDPGRDALDTVVQKGFPIEFIEQVRNIS
                     SQDAGACARAMKSRNRVVIDDIETDALSEPERRTAAVGGYRAVQSTPLLSRGGNLLGV
                     LSTYCRSPSKLTERDFKILDLYGRFTADLIERLQVEKTLRESEERLRLATQFGKVGVW
                     DWDLIANRMSWTESLYVIYGVRPAGFSPTVENVVSMIHPDDRERVLGLIEKILRSETA
                     CELEFRAVQPSGDIIWLFTNATALSDKGRPVRMLGATVDITERKRVEQALHESEERFR
                     DMADHAPMMVWVTEPDGSCSFVSKSWYKFTGEEPESGLGYGWTNSLHPDDQSDAHQTF
                     LAANRRQAPFRLEYRLRRADGEYRWMIDAAAPRFSKDGTFLGYIGSVIDITERKGIED
                     ALKEADRRKDEFLANVSHEIRSPMTAILGYADILLATLQNPDSVECVATIKQSGNYLL
                     EIINDLLDLSKIESGKITLQNEPISVPVLLNEIHSLMSVRAKEKRLRLSLSYDGPIPE
                     TVTSDRIRLRQILINLCANGIKFTEAGTVQITSRFLPESSALQFEISDTGLGIPPHMQ
                     TRLFQPFTQADTSATRAFGGTGLGLAITKRLVDLMRGSISFETELDKGTTFKVTIPVE
                     TSQKKEFNGASKDHRADQPTQCSRIDCRVLVVDDRPEMRSLFRYFIEGAGGRVSTAED
                     GRTAVEAVRSAETEGQPIDLVLMDIQMPGLDGYEATRNLRAEGFANAIVALTASAMKT
                     DRERCLAAGCNDYLTKPVQPDQLIDLIGRYCHS"
     gene            complement(4192..4548)
                     /locus_tag="VF182_21965"
     CDS             complement(4192..4548)
                     /locus_tag="VF182_21965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6439799.1"
                     /translation="MPKTVLVVDNEFGSRRNVAFLLREQGYAVEEADDGLTALDLLDK
                     KTFDLMICDVVMPRLSAFEVVQRIESSSTSTSIILITGHPELLDEKGLGHLPCFTKPF
                     NLYDLLRRVKQVLGDD"
     gene            complement(4751..5104)
                     /locus_tag="VF182_21970"
     CDS             complement(4751..5104)
                     /locus_tag="VF182_21970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439800.1"
                     /translation="MFMKSEREKREEEIERLMREAGLKREPDIAFQEAIKKAAAAIVE
                     EDAKKVAKTERVPRDWSISPTIAGLWLVVLGAGVAFSMPSVGAALILCGIAVFVWATF
                     LRSPKKKAPRRRIQT"
     gene            5300..6562
                     /locus_tag="VF182_21975"
     CDS             5300..6562
                     /locus_tag="VF182_21975"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6439801.1"
                     /translation="MPKLTRLFYGWRMVAAGCALRVLGSGLHSFGFTVFFLPLTQDLN
                     LNRTSTSLAFSLARAEGAIEGPIVGHLLGRFGPKPIMLAAVLLMGTGYLLLSQVESYI
                     SFLIVYLGVISLAHAGGFMHAPMVLINTWFIRHRARAITVSSAAFGLGGVLIAPILSV
                     IVQAWGWRWGAAIAGVVFLVIGIPLALTIRRSPESMGLLPDGDMPLSHTSDNDGSGHK
                     IKSEREVTLAEALRSFAFWGSVLAAGIRNSSYHAIGVHFVPLMVWKGLSQSEAALLLS
                     VYAFLGMALTLILGWFADKANKPRLTAFILFAAAGAMFLPIAGDSLWLLCLFTIFFAA
                     VETTFPLGWALVGDLFGRKHYAQIRGYMTMFYTWGGVIGPVVAGAIFDRWKTYEPLLW
                     SLVIVYIVAGLFFASLNRSWLKATGQVG"
     gene            6835..8031
                     /locus_tag="VF182_21980"
     CDS             6835..8031
                     /locus_tag="VF182_21980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6439802.1"
                     /translation="MEFRLSPEEESLRKTVEDFVRERLIPLEPQFEDAPDIFEGSRWK
                     SRARLCRDPVVLRYIDIMEDLEKQADDQGLWHLDVPKEYGGMQVSNVAMLAVTEELEK
                     SAIPFEFGNHVSNILYNCKGDQIDKFLWPCIRGEKTSAFGLTEPASGADPSMLQTTAV
                     PDGDHFIINGAKMFPTFADVADFVQLFARLPNTKGREGVTCFLIETGTPGYRVTRSIE
                     TIAGSEPCELVFEDCRVPRSQVLGEVGNGWALNQAWLGARRFQVGIRAHGMAQRVLRI
                     VGQLLGADSAEASKFVERIGHFLGELDALRTLSYFGAWKADQKMDVRSEAANVKLFGT
                     ELLHEIVDFALEIAGPAGLRKSHTLARYFRHARARRIIEGPSEIQRHIIQRALFREGV
                     AGLELP"
     gene            8087..9217
                     /locus_tag="VF182_21985"
     CDS             8087..9217
                     /locus_tag="VF182_21985"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX6439803.1"
                     /translation="MEFRLSEEEKLIQSTAAQLVKKELLTREGDYLRQRELFLPAGDP
                     PRRELDPQVRQTLINIAQRLGLWTLELPESGAEPIGYLARVLVYREFGRTILPFEPAC
                     APSLLAKTDFPKDLAAGKLSLALAFDQIHKTGSLDGIDTRYRPGAAGFRISNSEITIY
                     HPSADLFLLPAREQGTHRIGLFLIQKNSEGISIEDDVDLTNDARMARLKLRNFEVPES
                     GLLGYEYEAGEILAAEQLRIAARCLGIGMRCLADAIEHARTRETFGRTLAQRQAIQWM
                     LADLSVNLRGCTWLTLEAASRADQSLPFFDAAALAKKRAAAMAFEAADTAIQIHGGYG
                     VCKEFPFEGFYREARMLRLLYGREAELNRAMGQTFLKLGLKH"
     gene            9268..10416
                     /locus_tag="VF182_21990"
     CDS             9268..10416
                     /locus_tag="VF182_21990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX6439804.1"
                     /translation="MRRKRVCLVSYGSTEYSRKSEDSVLTQYANAIRQALERAGLHKK
                     EVRGLSITTQASPDFSPHVAEQLGLEVDWVLNGDYGGAGGVMSARRAADAIECGHLDL
                     AVLVGGNSFDKSLPNQRPLEYQRANFVDVYGYGGPNSLMALIQRLHMENYGTTLEQIG
                     KIAVAQRENALNNPQALFRQPMSMDDYLNSRMISDPIRLFDCVMPCSGAECVILASEE
                     KAKKITDKLVYLVTDAEISHYQVANMKPDKTTFGMKVVGDRIFSEIRHEEIDLLEIYD
                     DYPIAVMIQIEDLGFCKKGDGGKFVDAHGLTYKGDFPVNTGGGELSVGQAGLAGGFLH
                     IVEALRQLRGEADGHQVKKAERALVTGIGWLNYGRNLGTTAALVMERR"
     gene            10419..10838
                     /locus_tag="VF182_21995"
     CDS             10419..10838
                     /locus_tag="VF182_21995"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX6439805.1"
                     /translation="MAEARMPQKSIPKVNKVDQPFWEGAAGNKFLLQKCRSCGKVQFF
                     PRVACVDCFGTLDWIEAKGTGNIHSFTLVRVPRNPAFKDEVPIYYINVILDEGVIVES
                     KLVGEKREKVKLGDKVKVVFEQTHNPEIRLPCFELAG"
     gene            10875..11942
                     /locus_tag="VF182_22000"
     CDS             10875..11942
                     /locus_tag="VF182_22000"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439806.1"
                     /translation="MKLRRLETFLSDNLKSEIQNPKLHFTVYIQTVILILTVSGSLVP
                     SLLLAQSKSFRELNIGYPFGGSTSYFWVAHRSGSFEKHGIRVRPIFIRGGVQGVQALL
                     ARDVLIEMQGASAIVAAWAQGAKELRYVGAVGNKLDYILVGNSTIKTPADLKGKRIAV
                     SQIGSSSDFVARYALRQVGLNPEKDVTIVAVGAAGERWAALTGGHVQGTIVQPPLTLL
                     ARKAGLPIFVDLAKVDFEYTISGVVTTTSFIRAEPETVMNFMRGLADGMDFYRDENNK
                     EKVLEYLGQYFRSNAIEELEETRRAYSQVTPGLPFITPKAIENVIANDKNLAGMNLKA
                     DELLDLSFLQKLQQERKGKTR"
     gene            11958..12752
                     /locus_tag="VF182_22005"
     CDS             11958..12752
                     /locus_tag="VF182_22005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6439807.1"
                     /translation="MDFQTILFESRDRIAFITLNRPDSLNAMNRQMTRELVDVCQQVD
                     DDSGIRIAIFTGAGEKAFSAGMDLKERAETSFSPIERRQQKLTNKIYTQARAVAAITK
                     PTIAAIRGYCVGGGLEFALACDLRVAAEDAKLGLTEVRRGIIPGAGGTQRLTRAVGVT
                     KAMEMCLTGDTVSGLEAKSLGLVNNAVPSADVLKAAEDLAGRILKGAPMSVAFIKEAI
                     KKGVELPLEEGLRLEADLSALIGITEDAKEGPRAFAEKRAPVWQGK"
     gene            12925..13740
                     /locus_tag="VF182_22010"
     CDS             12925..13740
                     /locus_tag="VF182_22010"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6439808.1"
                     /translation="MAEEKVLYEVRNQTAWITLNRPESLNAVNREMVRSIADYCRGAE
                     EDPNVQIVVFKANGERAFSVGGDIKDRARQNATETEPAESPLVQRQSKNSAIPGTPAP
                     VIASMNKITIALLHGYTVGTGLLMSLACDIRIAAEGARLGLSEVRLGFLAGSGGTQRL
                     PRLVGIPKALEICLSGELYDAAECYRIGLVNQLVSYDQLVPAGEKMAQSFLKGAPLAL
                     RYIKEAIYKGSEMSLEQGLRYESDLQSMVMQTEDAKEGPKAFVEKRPPLWKGR"
     gene            14096..15622
                     /locus_tag="VF182_22015"
     CDS             14096..15622
                     /locus_tag="VF182_22015"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6439809.1"
                     /translation="MEKFLLMGDGLRRNASKFPLKIAAKDRFREIIYGDLNSRVNQLA
                     HALLSMGVGKGDGVALSVGNCIEHLEITFATAKIGALAIPLDIKWKALELGSVLSFFK
                     PRLLILQPDCAPELESAKTTQDLSFLKPIVIEDLSYGGLLTGQSTDEPAVEVNEDDPF
                     AVLLTSGTTGFPKGCLSTQKTFVFHCINNAIEKGLGVHDTAVLSSPIYFNAGRSFTLG
                     ILYFGGTMILHERFDAEDVLKTVEREKVTYLGAVPTMCERMLHVLESTKYDTRSLRCL
                     AITGGKVHPSVLEQLRRKLTPNIYRTYASTDSGQMAISKPSDMDYKPDAAGRPVWCVD
                     LRIVGDGDRPVKVGEIGEIVCQSPLATQGYYMNPEATHASFRDGWFYTGDLGYFDEEG
                     YLFVAGRKKDMVKSGGISIYPLEIESVIYSHADVLEAAVIGVPDAQWGEAVKAVVVLK
                     PGSSMQGEELVAFCKARLAPYKVPKSVEIFNSLPHTEVGKVNKVRLKEIILGQADRSS
                     "
     gene            15756..16838
                     /locus_tag="VF182_22020"
     CDS             15756..16838
                     /locus_tag="VF182_22020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439810.1"
                     /translation="MKDFMRACLYVLSGLILFLNTASAAERPFYQGKTITFFINFSAG
                     GPTDIEGRIVARHLARHIPGNPTIVVQNMSGGGGVTGINFLGEVAKPDALTLGYFTGP
                     YNHQMMKSTTLRVDLMKVPFISAVQGVTVCYIRSDVPPGIKKPTDLATAERFRAGGLS
                     FDSNKDLRFRLAFDILGLKYDYVTGYNSSNDARLAVQRNEIQYHDENIPGYRGVVEPQ
                     MVKTGMVTPLYYHDVIRPDGTMVKSPDYPELSSFTEVYTQIHGKAPTGIKYEALKAAN
                     MASQNLNRVALLPPGSPPEAIATMRQAFAALAKDEEFIADAKKVMRFQPRFETGEDGE
                     KLRDKVLTAPAEVVDFVRKFVEEAKK"
     gene            16879..17883
                     /locus_tag="VF182_22025"
     CDS             16879..17883
                     /locus_tag="VF182_22025"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439811.1"
                     /translation="MNQDGWTRLFVKVVALNFVLSLIAVTNVAGQEKVKFPVSASSKT
                     LGYSPLWVAHRQGFFDQEGLDVQLVLVSGADKSTMALMGGSVFVSSGAIDAIIGATEQ
                     GADLVSLGGVINGLTHVLLAGKKIKTYEDLRGANIGSSGLTSGTAFVLRRMMAANGLQ
                     YPKDYNLINVGPSAQSFLALTANRVDAALIAVPLSSDAAEMGYNVIGRAADIIPHYQL
                     TEVSTRRAWAEKNRPLMVRFMKGMVLAMRWLYDNKEPAIEFLAKEMKLKPDHARRGWE
                     YYTENRIWNPNAEANLEGVKAVIQIMAERGQLKPPLPSPAKYIDHSYVEEALKELGRR
                     "
     gene            17967..18833
                     /locus_tag="VF182_22030"
     CDS             17967..18833
                     /locus_tag="VF182_22030"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6439812.1"
                     /translation="MNLELRDHYFAHDGLKLHYWEWGDPNEETFVFVHGVRDQGRSWD
                     DFIAAMIARGLPIKHAVAIDLRGHGDSEWPSTSRGYQHEDFLTDLAGLLRHLNKEPLT
                     IIGHSLGGSMCLLYAGTFPDKVKRMVLLESLGPFARADEEVPEIFAERLKGRDYVEIP
                     FPHESLEAAATALRKTYPLIPDNAALHMARYGTSLKGGRYRWKYDPILRYRTTTALSE
                     GQIEAFIRRVQCPIFFVYGTESDFMKSVRGPRVKLFPNAEIVPVEGAGHHIPHERPEE
                     LAEIVVPFLLRA"
     gene            18912..>19121
                     /locus_tag="VF182_22035"
     CDS             18912..>19121
                     /locus_tag="VF182_22035"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6439813.1"
                     /translation="MAELRTILQKIDEEAGVAWLTFNRPEKKNALSQRLMIEMVEVLK
                     ELGDNDKIKCIVTTGSGDSYSSGLDL"
BASE COUNT         4391 a         4920 c         5489 g         4321 t
ORIGIN      
        1 ggcggacgcc tgcggcgcgc ggatctttga tttacccttg tcaccggaaa aggtcttctg
       61 ggccctgcac ggcaagatca acaacagaga tacccggcca ggtggtgatc caatcaagcg
      121 agaagccaga gtgaaacttg cctaacttgt cgaggccagc gggccctagt tctccataat
      181 gagagcgtcg gcgaaacgct accagccatc gagccgtttc gtcccaggct ttctcatcgg
      241 tcggtcctgc ggtcggaccg ttctggtggt gaagaggcaa gttacttctc atgtgaacga
      301 ctgatctcag atttatgaac ccggctgccc ggcagcccgg tgacagattc tctccgttca
      361 attctatgca agcgatcaat gccgctgtta gctcgcgcgc gcaggaacag ctagtccgac
      421 acaagtattc atccacttga cgcaagcgat agccggcggt attgatgtgc ccgtacgaca
      481 gctgcgattg cacaatggcg ttcaacgggc accttaaaag atccgctaga cgtcaaacaa
      541 atgactctta gatttctgct gcgccatcct ccattgtcaa cttcgtagcg cgtgctccgc
      601 ccggcgccgc ctttgctttt agtgacgagg aaagattgcc gcgatactcc ctgcacacgc
      661 gacagagtca tacgatgaac gtcatgacaa ctcatgaacg atcgctgatg gagttccgcc
      721 tttgtccgct agcttaaatt ttagctcctc aggagtactg gcagaaagag ccattggtca
      781 tggagactga gtcggagttt cgagttctat ttgaggccat accgggactg gttgttgctt
      841 tgcgccccga tcctccggca ttcacaatca tcgccgcgac caacggctat ctaaaagcca
      901 ccggcactag gcgcaaggat attgtcggtg gtggtttctt tgatgttttt cccggttgcc
      961 gcggtgaagc tcgtaacaac gctacggcca atctgaagcg gtcttttgaa acagtattgc
     1021 gcaaccggag cgccgatatc atggcgatac agaaatatcc tatttgcccg gtggaactaa
     1081 gtggtgaagg ttgggaagag cgctactggc gtggaatcaa tgctccggta ctgggcccga
     1141 gcggtgaagt cgcctacatt cttcaccgcg cgcaagacat gaccgaattg gtgcggctta
     1201 aagagcagga gaaagagcac gagcaattga agcacgagct tcaacttcgc gctgaaaagg
     1261 cggaagcaaa gcaattggcg cgggagcaac agattcaacg cttgaaccgg cacttgcgca
     1321 gagctttgac ccgacaacag aaatcacgga cggaagcgta ccttcccaaa gattggttgg
     1381 agcatgtgct tttggaagtc cacgtgcagg tattcgccct cgatcgtaag tggcgctatg
     1441 tctacgtgaa cgagcgtgtg acgcaggtcg tcgggctgac caaggcgcaa atgctcggca
     1501 aaactatctg ggagttgttt cccgaagtcg ttggcagcgt cttcgagcgt cagatccggc
     1561 aagcagcggc agaattcagt ccggttcatt ttgagtactg ctatccgcca tggaatcgct
     1621 ggtttgagat gcatgtctat ccttcggcgg agaggctaac attttttgtt gccgagatta
     1681 ccgatcgaaa gaaggcagaa gccgcgctgc aggaaagcga agagcggctg gctggagagc
     1741 ttgtcaccat gaatcgtttg cacgagctca cgctacggct cttcagcgcc gtcgacttac
     1801 gtgcagcgat ggatgaagtg ctcgaagcgg tgacggcgct tatgggcacg aacatgggtg
     1861 acatacggat ctatgacccc ggccgcgacg cgttagacac ggtcgttcaa aaaggtttcc
     1921 cgatcgagtt cattgaacag gttcgaaaca tctcgtcgca ggatgcgggc gcatgcgccc
     1981 gcgcgatgaa atcgcgcaat cgggtcgtaa tcgacgatat cgaaacagat gctttatccg
     2041 agccggaacg tcgaacggcc gccgtcggcg gctatcgagc cgttcagtcg acaccgctgc
     2101 ttagccgagg cggtaatctg ctgggcgtgc tgtcgacata ctgccgaagc ccaagcaaac
     2161 tgacggaacg tgatttcaag attttggatt tgtacggtcg ctttaccgcg gatttgatag
     2221 agcgactgca ggttgaaaag acactgcgcg aaagcgagga gcgtctgcgc ctggcaactc
     2281 aattcggcaa agtcggtgtc tgggattggg acctgattgc caatcggatg tcatggaccg
     2341 agtcccttta tgtcatttac ggtgtccgtc cggccggttt cagccccacg gttgagaacg
     2401 tcgtctcaat gattcacccc gatgatcgcg aacgcgtcct aggtttgatc gaaaaaattc
     2461 tccgcagcga gaccgcctgt gagctcgagt tccgagcggt tcagccatcg ggcgatatca
     2521 tttggctatt taccaacgcc acggcgttgt ctgacaaggg gcgtccagtg cggatgcttg
     2581 gcgcgaccgt cgatattacc gagcgcaagc gagtcgaaca agcgctccat gaaagtgaag
     2641 agcgcttccg ggatatggcc gaccatgcgc cgatgatggt ctgggttacc gaacctgacg
     2701 gtagttgcag tttcgtcagt aagtcgtggt acaagtttac cggtgaagaa cccgagtccg
     2761 ggcttggcta cggctggacg aactcacttc atcccgacga tcagtccgat gctcatcaga
     2821 cgtttctcgc tgccaatcgt cggcaggcgc catttcggct ggagtaccgc ttgcggcgcg
     2881 ccgatggcga ataccgttgg atgatcgatg cggcagcgcc ccggttcagt aaggatggaa
     2941 cctttctcgg atacatcggc tccgtgatag acatcaccga gcgtaaagga attgaagatg
     3001 cgttaaagga ggcggaccgg cgcaaagatg agtttctcgc caacgtgagt catgaaatcc
     3061 gcagccctat gacggctatc ttaggttacg ccgacatctt actggctaca ctgcaaaacc
     3121 cggatagcgt cgaatgcgtc gcgacgatca aacagagcgg caactacctt ctggaaatca
     3181 tcaatgatct cctggatctt tcgaagatcg agtcgggtaa gatcacgctt cagaacgaac
     3241 ccatctcggt gccggtgtta ctcaacgaga ttcactcgtt gatgtcggtc cgggccaaag
     3301 aaaagagact gcgattgtcg ctcagctatg acgggcctat ccccgagacg gttaccagtg
     3361 atcgaatacg gctgcgtcaa attttgatca atctttgcgc caatgggatc aagttcaccg
     3421 aagccggcac agtccagatc acgagccgat ttctgccgga aagctccgcg ctgcagttcg
     3481 aaatctcgga cacaggatta gggattccac cgcacatgca gacgcgtctg tttcaaccgt
     3541 ttacgcaagc cgatacttcg gcgacccgtg cgttcggcgg aactggcctg gggctggcga
     3601 tcaccaaacg cttggtcgac ctgatgagag gcagtatttc tttcgaaacc gaactggaca
     3661 aagggaccac tttcaaagta accataccag tcgagacgtc tcagaaaaaa gagttcaacg
     3721 gcgcctcgaa ggatcaccgg gccgaccagc caactcaatg ttcgcggatc gactgccgtg
     3781 tgctcgtggt ggatgatcgt cctgaaatga gatccctgtt tcgctatttt atcgagggag
     3841 ccggggggcg tgtttcgacc gcggaggatg ggcgcactgc agtcgaagcg gtgcgcagcg
     3901 ccgagaccga aggtcagccc atcgatcttg tcctcatgga cattcaaatg cccgggttgg
     3961 acggctatga agcgacccga aacttgcgcg ccgaaggctt tgccaacgca atcgtcgccc
     4021 tcacagcctc agccatgaaa acggatcgag aaagatgtct agccgctggc tgtaacgact
     4081 atctgaccaa accggtgcaa cccgatcagc tgatcgatct gattgggcga tattgccact
     4141 cctaatgtac agtttccgag tcgctgccgt ctgacaatca aactgaaccg atcagtcgtc
     4201 accaagcacc tgttttaccc tgcggaggag atcatacaaa ttgaatggtt tggtgaaaca
     4261 gggtagatgc ccaagccctt tctcatcaag caattcaggg tgcccggtga tcaggataat
     4321 tgaagtggat gtggatgagg attcgattcg ctggaccacc tcgaacgcac tcagtcgggg
     4381 catgacgaca tcgcatatca ttagatcgaa agttttttta tccaacaagt ccaatgcagt
     4441 gagcccatcg tctgcctcct cgactgcata accttgctcc cgtaaaagaa acgcgacgtt
     4501 tctgcgactg ccaaattcgt tatcgacaac aagaaccgtt ttgggcattt tctttgagag
     4561 gctctggtat ggcagtcacc acataacata tccgaacgat taagcgcgag agaataacgg
     4621 tttaatcaga aatgtcacaa cccgagggac ttgcccgcag atctggattg agccgctgga
     4681 gcgatgttgc cgccggtcac ttgccggatg ttgaagtata gtcgcgatcg tatcgcgaca
     4741 ctgaagacgg ttaggtctga atgcggcgac gcggcgcctt ctttttgggt gatcgtagaa
     4801 aagtggccca gacgaagaca gcgataccgc agagtatcag cgccgcgccc acgctcggca
     4861 ttgaaaacgc cacacccgct ccgaggacca ccagccacag cccggctatg gtcgggctga
     4921 tcgaccaatc gcgtggaacc cgctccgtct tcgcaacttt cttggcatct tcctcaacga
     4981 tggcggccgc ggcctttttg attgcttcct gaaacgcgat gtcgggctct cgttttaggc
     5041 cagcctcccg catcagcctt tcgatctctt cttcccgttt ttcacgctca gacttcatga
     5101 acatagtctt tcgaagattc gctcagctgt caacaacttt catttagttg ataatcgaag
     5161 atttatgatt tgccggggct gaaaaacaag ctaacaaata cggcgcgcta gcaggcaagc
     5221 agtttggctt ttcctccacg ccaggtcgtg ctaaatcaac gttgaagtaa tttccaatca
     5281 tcctacagga cctgtctctt tgcccaaatt aaccagactt ttctacggct ggcgcatggt
     5341 ggctgccggg tgcgctctgc gcgtgttggg gtccggactt catagttttg gctttaccgt
     5401 gttctttctt ccgctgaccc aagatttgaa cttgaatcga acctcaactt ctctggcatt
     5461 ttcactggcg cgcgctgaag gagcgatcga aggccccatc gtaggccacc tgctaggccg
     5521 tttcggtccc aagccgatca tgctggccgc ggttctcctc atgggcacgg gttacctgct
     5581 gctctcccag gtcgagagct acatcagctt tttgattgtt tacttaggcg tgatttctct
     5641 cgctcatgcg ggcggattca tgcatgcgcc gatggtcttg atcaacacat ggttcatccg
     5701 ccatcgggct cgcgccatta ccgttagcag tgccgctttc gggctgggcg gagttctcat
     5761 tgctccgatc ctaagcgtca ttgtccaggc gtggggatgg cgttggggag cagccatcgc
     5821 cggagttgtg tttctggtga tcggtatacc gttggccctg acgattcggc gctcccctga
     5881 gagcatggga ctcctgcccg acggagacat gcctttgagt catacctccg ataacgacgg
     5941 cagtgggcac aaaatcaaat cagaacgcga agtgacgttg gccgaggcgc tgcgctcatt
     6001 cgccttctgg ggctccgtcc tggcggcggg cattcgcaat agttcgtacc atgcgatcgg
     6061 cgttcatttt gtgccgttga tggtatggaa aggcttgagt caatcggagg cggctttatt
     6121 gctgagcgtt tatgcgtttc tcggcatggc gttgactctc atcttgggat ggtttgcgga
     6181 taaagccaac aagccgcgct taaccgcttt catcttgttt gccgcggccg gcgcgatgtt
     6241 cctgccgata gccggagact cgctctggtt actgtgcctg tttactattt tctttgccgc
     6301 tgtagaaact acttttcctc ttggttgggc gctggttgga gatttgtttg ggaggaagca
     6361 ttatgcccaa atacggggct atatgactat gttttacacc tggggaggag ttatcggtcc
     6421 ggtggtagct ggcgcaatct tcgaccgctg gaagacctac gaacccttgt tgtggtcatt
     6481 agtgattgtg tacatcgtcg ccggactgtt cttcgcaagc ctcaatcgat cctggctcaa
     6541 agcgacgggc caggtcggct agagccggcg gcatcggcgt ttgctcgatc cgggcgagtg
     6601 cgtcattcca cgatcttaag ctttagatga gttgatcggg ttcgcgggct aaaagcaatt
     6661 tctctccaag taaaaagcgg cgcaaacttc taagctaaga cagtgtcact agcatgggga
     6721 aataaatgat tttatctttt ggtttgtttt caggtgtggt aaggggtgct ggacactacc
     6781 ataatcgatc gcagtaaaac gattatggtg attgtccgac gtgaggatgt ctgaatggaa
     6841 tttcgcttat cgcccgaaga agaaagtttg cgcaagaccg tcgaggattt tgtccgtgag
     6901 cgtttgattc cgctcgaacc gcagttcgaa gatgctcccg atattttcga aggcagccgt
     6961 tggaagagcc gcgcgcgact gtgccgcgat ccggtggttc tgcgctatat agacataatg
     7021 gaagatctgg agaagcaggc tgacgatcaa gggctttggc atctcgacgt tccaaaagag
     7081 tacggcggga tgcaagtgag caatgtggcg atgctcgcgg ttaccgaaga gctggaaaaa
     7141 agcgccatcc cgttcgagtt tggcaatcac gtatccaata ttctttataa ctgtaaaggc
     7201 gatcagatcg ataagtttct ctggccctgc attcgcggcg agaagacctc ggccttcggc
     7261 ttgaccgaac ctgcctcggg agccgacccg tcgatgctgc aaaccaccgc agtgccggat
     7321 ggcgatcact tcattatcaa cggcgcgaaa atgtttccga ccttcgctga tgtcgcggat
     7381 ttcgtgcagc tgttcgcccg tttgccgaat accaagggga gagaaggcgt gacctgtttt
     7441 ctgatcgaaa ccgggacgcc aggttatcgc gtgacccgca gcattgaaac gattgccggc
     7501 agcgagcctt gcgaactggt cttcgaagat tgccgcgtgc cgcgcagtca agttctcggt
     7561 gaggtcggca atggctgggc gctcaatcag gcatggttgg gagcgcgtcg ctttcaagtc
     7621 ggcattcgcg ctcacggcat ggcgcaacgg gttttgcgaa ttgtcgggca actgctcggc
     7681 gccgattcgg cggaggcaag taaattcgtc gagcgaatcg gccatttcct aggggaattg
     7741 gacgcgctgc ggacgttgag ctatttcggc gcatggaaag ccgatcagaa gatggacgtc
     7801 cgctcggaag cggctaacgt gaaattgttc ggcactgagc tgttgcacga gatcgtcgat
     7861 ttcgcgctgg agatcgccgg ccctgcgggg ctgcgcaaga gccatacgct ggcgcgctat
     7921 tttcggcatg cgcgggcgcg gcgcatcatc gaagggcctt ccgagataca gcgccatatc
     7981 atccagcggg cgctgtttcg tgaaggcgtt gccgggcttg aattgcctta acgcgggtta
     8041 atcggctact aacgtcaatt tcgagaagct tcgatttggc ataatcatgg aatttagatt
     8101 gtcggaagaa gagaagctga ttcagagcac agcagctcaa ttggtaaaaa aggagctcct
     8161 cacgcgggaa ggagattatc tcagacagcg ggaactcttc ctgccggctg gggatccacc
     8221 gcgccgtgaa ttggacccgc aggtccggca gacgttgatt aatatcgcgc agcgtttagg
     8281 cttatggacg cttgaactgc ccgaatccgg agcagaaccg atcggctact tggcgcgggt
     8341 tctggtctac cgtgaattcg gtcggactat cttaccgttc gagccggcat gcgctccgtc
     8401 gctgttggcc aagaccgatt ttcccaagga cttggcagcc ggcaagctgt cgctcgcact
     8461 ggcgttcgat cagattcaca aaaccggctc cctcgatgga atcgacactc gttatcgtcc
     8521 tggagccgca ggcttccgca tcagcaacag cgaaatcacg atctatcatc catccgccga
     8581 tttgttcctg ctgcctgccc gggaacaggg gacccatcga atcggtttat ttttgatcca
     8641 aaagaatagc gaggggatct ccatagagga tgacgtcgat ttgaccaacg atgcgaggat
     8701 ggcgcggctg aagctgcgca acttcgaagt tcccgagagc ggcttgctcg ggtacgaata
     8761 cgaggctgga gaaattctcg ccgccgagca gctacgcatc gccgcgcgct gtctcggtat
     8821 cggcatgcgc tgtttggctg atgcgattga acatgcgcga actcgcgaaa ccttcggcag
     8881 gacgctggcg caacgccagg caatccagtg gatgctggcg gacttatcgg taaatcttcg
     8941 cggctgcaca tggctgacgc tggaagccgc gtcgcgcgct gatcagagct tgccgttttt
     9001 cgacgccgct gcgctggcaa agaaaagagc cgcggccatg gcgttcgagg cggcggatac
     9061 cgcgattcag atccatggcg gctatggtgt ctgtaaagag tttccgttcg aagggttcta
     9121 tcgcgaagcg cgcatgctgc gcctgctcta tggccgtgaa gccgagttga accgggcgat
     9181 gggtcaaacg tttttaaagc tgggcctgaa gcattaggcc ggcgagattc aacagaaagt
     9241 ttatcagaga ccaacgggga gaggatcatg cgacggaaaa gagtttgctt ggtgagctac
     9301 ggttcgacgg aatactcgcg aaaatcggaa gattcggtgt tgactcagta tgccaatgcc
     9361 atccgccagg cgctcgaacg cgccggcctg cataagaaag aggtgcgcgg gctctcgatc
     9421 acgactcagg ccagtcccga cttttcgccg cacgtcgcag agcaactggg gctggaggtc
     9481 gattgggttt tgaatggcga ttatggcggc gccggcggag tcatgagcgc gcgccgggct
     9541 gcggatgcca tagagtgcgg ccatctcgat cttgccgtgc tggtgggcgg caactcgttc
     9601 gataagagcc ttcccaatca aaggccgctc gaataccagc gcgccaactt tgtcgatgtt
     9661 tacggttacg gcgggccgaa ttcgttgatg gcgctgattc agcggttgca tatggaaaac
     9721 tacggcacga cgctcgaaca gatcggcaag atcgccgtgg cgcagcggga aaacgcgctc
     9781 aataaccctc aggcgttgtt ccgccaaccg atgagtatgg atgattatct caactcgcgg
     9841 atgatttccg atccgattcg gctcttcgat tgcgtcatgc cgtgttccgg cgcggaatgt
     9901 gtgattttgg catccgaaga gaaagcaaaa aagatcaccg acaagctcgt ttatctggtg
     9961 accgacgccg agatctcgca ttatcaagtc gccaatatga agcccgacaa gaccaccttc
    10021 ggcatgaagg tggtcggcga tcgcatcttc tcggaaatcc ggcacgagga gatcgatctt
    10081 ctggaaatct atgacgacta tccgatcgcg gtcatgattc agatagaaga cttgggcttt
    10141 tgcaaaaaag gcgacggtgg aaaattcgtc gatgcccacg gtctcactta caaaggtgat
    10201 ttcccggtga acaccggcgg cggggagctg tcggtgggcc aggctggcct ggccggcggt
    10261 tttctccata tcgtcgaggc gcttcggcaa ttgcgcggtg aagcggatgg ccaccaggta
    10321 aaaaaggccg agcgcgcgct ggtcaccggc atcggttggc tcaactatgg gcgcaatctc
    10381 ggcaccaccg cggcgctggt gatggaaagg agataatgat ggcggaagca agaatgcctc
    10441 agaagagcat acctaaggtg aataaggtcg atcagccgtt ttgggaaggc gctgccggca
    10501 ataagttcct gctgcagaaa tgccggagct gcggcaaagt gcagtttttt ccgcgcgtcg
    10561 cctgcgtcga ttgtttcggc acgctcgatt ggatcgaggc caaaggcacg ggcaacattc
    10621 atagctttac cttggtgcgt gtcccgcgca atccggcctt caaagacgag gtgccgattt
    10681 attacatcaa tgtgatttta gacgaaggcg tgatcgtcga gagtaaactg gtcggggaga
    10741 agagagagaa ggtaaagctc ggcgataagg tgaaggtcgt ttttgagcag acgcacaacc
    10801 cggagatcag actgccgtgt ttcgagttgg cgggatgacg attttggatt ttggattgaa
    10861 gatcggagga tagaatgaag ttaaggcgcc tagagacttt cttatccgat aatttaaaat
    10921 ccgaaatcca aaatccaaaa ttacacttca cggtttacat acaaacggtt attctaattc
    10981 ttaccgtatc aggttcttta gtgccttccc tgcttcttgc tcagtccaaa tcttttcgcg
    11041 agctcaacat cggctatcca ttcggcggtt cgaccagcta tttctgggtg gcgcatcgtt
    11101 ctggatcatt tgaaaaacac ggcatccgcg ttcggccgat cttcatccgg ggcggggttc
    11161 agggcgtgca ggcgctgctt gcgcgggatg tgttgatcga gatgcaaggc gcatcggcga
    11221 tagttgccgc ctgggcacaa ggcgctaagg agcttcgata cgtcggcgcc gtcggcaaca
    11281 agctcgatta catcctggta ggcaactcca caattaagac tccggcggac ttaaaaggga
    11341 aacgaatcgc agtaagccag atcggctcga gctcggattt cgttgcgcgc tatgcgctgc
    11401 gccaggtcgg tttgaacccg gaaaaagacg tgaccatcgt cgccgtgggc gccgccggag
    11461 agagatgggc ggccttgacc ggagggcacg ttcaagggac catcgtacag cctccgctca
    11521 ccttgctcgc gcgcaaagcc ggtcttccca tttttgtcga tctggccaag gtcgatttcg
    11581 aatacaccat ctccggggtg gtgaccacga cctcgttcat tcgcgccgaa ccggaaacgg
    11641 taatgaattt catgcgcggg ttagctgacg gcatggattt ttatcgcgat gagaataata
    11701 aagaaaaagt tctcgagtat cttggccagt attttagatc caacgccatt gaagagctcg
    11761 aagaaactcg gcgcgcctac agccaggtga caccggggct gccgtttata acgcccaagg
    11821 cgatcgagaa cgtcatcgcc aacgataaaa accttgccgg catgaattta aaagccgacg
    11881 agcttctcga cctgtcgttt ctgcagaagc tgcaacagga gagaaagggg aaaactcgtt
    11941 agaagaggaa gcactcaatg gatttccaaa cgatactttt cgaaagccgc gatcggattg
    12001 cgttcataac cctcaaccgg cctgattcac tcaacgcgat gaatcgacag atgacacggg
    12061 agctcgtcga tgtttgccag caagtggatg atgacagcgg gattcggatc gcgatattca
    12121 caggcgccgg tgaaaaggcg ttttcggcgg ggatggatct caaagagcgc gcggaaacat
    12181 ccttttcgcc catcgaacgg cgccaacaga aattaaccaa caagatttac acccaggcgc
    12241 gggcggtggc ggcgatcacc aagccgacga tcgcggcgat tcgcggttat tgcgtcggcg
    12301 gcggattgga gttcgccctg gcatgcgatc tacgggttgc ggcagaggat gccaagctcg
    12361 ggctcacgga agttcggcgt ggcatcatcc cgggcgccgg cggcacgcaa cgcttgaccc
    12421 gcgcggtcgg cgtcaccaaa gcgatggaaa tgtgtcttac cggcgatacc gttagcggcc
    12481 tcgaagcaaa gtcattgggc ctggtgaaca acgccgtgcc ttccgccgac gtgcttaaag
    12541 ccgcggagga tctggccggg cggatactca aaggcgcgcc gatgtccgtc gcgttcatca
    12601 aggaagcgat caagaagggc gtcgagttac cgctcgagga aggattgcga ttggaagccg
    12661 atctctccgc attaatcgga ataaccgaag acgccaaaga aggtccccgg gcattcgccg
    12721 agaagcgagc gccggtttgg caagggaaat aaaccaacga gttcaaaacg ctcaagtcgc
    12781 ttaaaacgtt caaccggtta tgagaagatc aaacgcttgg cagtctttca tcgttaaact
    12841 cggaatgagt catttgcggt ctgccggttt gaacggccgc aacttttgaa cgacttcaac
    12901 cctaagttat acggagcgag ttagatggca gaagagaagg ttttgtacga ggttagaaat
    12961 caaaccgcat ggataacttt gaatcgtccg gagtcgttga atgcggtaaa ccgtgagatg
    13021 gtaaggtcga ttgctgacta ttgccgcggg gcggaagaag accccaacgt gcagattgtt
    13081 gtattcaagg caaatggcga gcgcgcgttt tccgtgggcg gagatatcaa ggatcgtgcg
    13141 cggcagaatg caacggaaac cgaacctgcg gaatcaccgc tcgtgcagcg ccagtcgaag
    13201 aactcggcga ttccgggaac tccggcgccg gtgattgcgt cgatgaataa aattaccatt
    13261 gcgctgcttc acggttatac cgtaggcaca gggcttttga tgagtttggc gtgtgacatc
    13321 cgcatagcgg cggaaggggc gcgtcttgga ttgagcgagg tgcggcttgg ttttctggcg
    13381 ggttccggtg gaacgcagcg gctgccgcgt cttgtcggca ttcccaaggc gttggaaatt
    13441 tgcctctccg gcgaactcta tgatgccgcg gaatgttacc gcattggatt ggtgaaccag
    13501 cttgtatctt atgatcaatt agttcccgca ggtgaaaaaa tggcgcagtc gtttctcaaa
    13561 ggcgcgccgt tggcgctgcg ctatatcaaa gaagccattt ataagggcag cgaaatgtcg
    13621 ctggagcagg ggctgcgcta tgagtccgat cttcagagca tggtgatgca aaccgaagat
    13681 gccaaagaag gtccaaaagc ttttgtcgaa aagcggccgc cgctttggaa gggaagataa
    13741 atagccgatc catcgacctt gcttgggact ggtttggatt cctccggaag tgaccgcaaa
    13801 ggtgcaagcc atcgagccta cggcgatgag cctgtgtctt gaagcagctt gcgagctcgc
    13861 gatgatgcgg caaactctag ggaaaacttc caacgtttag ccaagcaccc aagacgtcat
    13921 tgaactttac attcggtgcc ttcgcctctc cgggcttacc ccctttgctc cgggaagttg
    13981 aacttatata gtctgggcgg atcgcggatt gatgaggact aagaagatca taggtgcgag
    14041 gttccggtcc gtttccgacg atccgagaat ctaaaaacca aaatctaaat cgaaaatgga
    14101 aaagtttttg ctgatgggcg atggcttgcg ccgtaacgcg tccaagtttc ctctcaaaat
    14161 cgccgccaaa gatcgtttcc gagaaataat ctacggtgac ctcaattcgc gggtgaatca
    14221 gctagcccat gcgctgctgt cgatgggtgt gggtaaaggc gacggtgtgg cattatcggt
    14281 gggcaattgt atcgagcatc tggagatcac ttttgctacc gcgaagatcg gcgcgctggc
    14341 gattccgctc gatatcaaat ggaaggctct ggaactcggt tcagtgcttt cgtttttcaa
    14401 gccacggttg ctgattctgc agccggattg cgccccagag ctcgagtctg ccaagaccac
    14461 tcaggatttg agctttctca agccgattgt catcgaggat ttgagttatg gcggtttact
    14521 gacgggacag agtaccgatg aacccgcggt cgaagtcaac gaggacgacc catttgccgt
    14581 tttgctgacg tccggcacca caggctttcc taaaggctgt ctctcgacgc aaaagacctt
    14641 tgtctttcac tgcatcaaca acgccatcga aaaaggcctc ggcgttcacg acaccgcggt
    14701 tctctcctcg ccgatttact ttaacgccgg ccgttcgttt acattgggaa ttctttattt
    14761 cggcgggacg atgatcctgc acgagcgctt cgacgctgag gatgttctca agacagtgga
    14821 gcgtgaaaag gttacgtacc ttggcgccgt gccgacgatg tgcgagcgca tgctgcacgt
    14881 gctcgaatcg acgaagtatg acacccgctc gctgcgttgt ctggcaatta ccggcggcaa
    14941 agttcatcct tccgtccttg aacagttaag aaggaaactc acgccgaaca tctatcgaac
    15001 ttacgcctcc accgattccg ggcaaatggc catctcaaaa cccagcgaca tggattataa
    15061 acccgatgcc gccgggcggc ccgtctggtg tgtcgatttg cgcattgtcg gcgatggcga
    15121 ccggccggtg aaggtcggcg agatcggcga aatcgtctgc cagagcccgc tggcgacgca
    15181 aggttactac atgaatccgg aggcgaccca tgcatcgttc cgtgatggct ggttctatac
    15241 cggcgatctc ggctacttcg acgaagaagg ctatctcttt gtcgccggcc ggaagaagga
    15301 catggtcaag agcggcggca tcagcattta tccgctggaa attgaaagcg tgatttacag
    15361 ccatgccgat gttttggaag cggcggtgat cggtgtcccc gatgcgcaat ggggcgaggc
    15421 ggtaaaagcc gtggtggtac taaagcccgg atcgtcgatg caaggagaag aactggttgc
    15481 cttctgtaaa gcgcggctag ctccctacaa ggttcccaag tccgtcgaga tctttaattc
    15541 cttgccgcac accgaagtgg gcaaagtgaa caaagttagg ttaaaggaaa tcattctggg
    15601 tcaagcagat cggtcgagtt gaagcaaatg cattgtgttc gttgcaattc ttaggtttac
    15661 atctacaggc ggttccataa gggattcttc tgactcccac cggcgaatgg tttagcgtca
    15721 aaaacggatc ggcgagaatg aaaggagagc taatcatgaa agatttcatg cgcgcttgtt
    15781 tatatgtcct ttcgggtttg atcctgtttc tgaatacagc ctcagcggcc gaaaggccct
    15841 tttaccaggg aaaaaccatt accttcttca tcaacttttc cgcgggcggg cccaccgata
    15901 tcgaaggacg gatcgtcgcg cggcatcttg ccaggcatat accgggaaat ccaaccatcg
    15961 ttgtgcaaaa catgtccggt gggggaggag tcaccgggat taattttctt ggcgaagtgg
    16021 ccaagcccga cgccctcacc ctgggttatt ttaccgggcc atataatcat cagatgatga
    16081 agagcacgac gttaagagtc gatctcatga aagtgccctt catctccgcg gttcaaggcg
    16141 tgacggtgtg ttatatccgc tctgacgttc cgccgggaat caagaaacct acggatctcg
    16201 ccacggccga gcgcttccgg gccggcggcc tgagcttcga cagtaataag gatctgcggt
    16261 ttcgactggc tttcgacatc cttggcctga agtacgatta cgtaaccggc tacaacagca
    16321 gcaacgacgc gcggctggcg gtgcagcgca acgagattca atatcacgat gaaaatattc
    16381 cggggtatcg cggcgtggtc gagccgcaaa tggtgaaaac cggcatggtc acgccgctct
    16441 attatcacga tgtcatccgg cccgatggaa ccatggtaaa aagtccggac tatccggagc
    16501 tgagttcatt taccgaggtc tatacgcaaa ttcatggcaa ggctccaacc ggcatcaagt
    16561 acgaagctct taaagccgcg aatatggcta gtcaaaatct caatcgtgta gcgctcttgc
    16621 ccccgggttc gccgcccgag gcaatcgcca ccatgcgtca ggcgtttgct gctttggcta
    16681 aagatgaaga attcatcgct gacgccaaga aggtcatgcg atttcagccg cgttttgaga
    16741 ccggcgagga tggtgaaaag ctccgggaca aagtcttaac cgctcccgcc gaggtggttg
    16801 attttgtccg caaattcgtc gaagaggcga aaaaatagtt ttgcagcgac gcagtcgaac
    16861 atgcagttaa gaaagaccat gaatcaagac gggtggacca gattgtttgt aaaggttgta
    16921 gcccttaact tcgttttatc tcttatcgct gttaccaacg tcgccggaca ggaaaaagtt
    16981 aagttcccgg tcagcgcttc ttccaagaca ctgggataca gcccgctgtg ggtagcacac
    17041 cggcaaggtt tcttcgatca ggaaggactg gatgttcagc tggtgttggt ttccggagct
    17101 gacaagtcga ccatggcctt gatgggcggc tcggtgttcg tatcgagcgg cgccatcgat
    17161 gccattatcg gcgccaccga gcagggtgcg gatttagttt cactcggagg cgtaatcaac
    17221 gggctgacac acgtcctcct ggcgggaaaa aaaatcaaaa cctacgaaga cctacgtggc
    17281 gcaaacatcg gttcgtccgg acttacatct ggaaccgcat ttgtcctcag gcgaatgatg
    17341 gccgccaatg gtttgcagta tccgaaggat tacaatttga tcaacgtcgg tccttcagcg
    17401 cagtcgttct tggcgttgac ggccaatcgg gtagacgccg cattgatcgc cgtgcccctc
    17461 agttccgacg ccgcagaaat gggctataac gtcatcggca gggcggcaga catcattccg
    17521 cactatcaac tgactgaggt ttccacccgt cgcgcttggg ccgagaaaaa ccggccattg
    17581 atggtgcggt tcatgaaagg catggtgctg gcgatgcgct ggctttatga caacaaagag
    17641 ccggcgatcg agtttttggc caaagagatg aagctcaagc cggatcatgc gcggcgcggt
    17701 tgggagtatt acacagaaaa tagaatttgg aatccgaacg ccgaagcgaa tttggaaggt
    17761 gtcaaggctg tgattcagat tatggccgag cgtggacagt taaagccgcc gttgccgagt
    17821 ccggccaagt acatcgatca cagttacgtc gaggaggcgc ttaaagaatt gggccggaga
    17881 tagtaaagag agtagatggc acgaacatcc agcctccgct cgatctcact gtcacaaatg
    17941 gtattggaaa ggcggtaaaa accagaatga atttagagct gcgggatcat tacttcgctc
    18001 atgatggttt gaaacttcac tattgggaat ggggcgaccc caacgaagag actttcgttt
    18061 ttgttcacgg cgtgcgcgac cagggacgaa gttgggatga tttcatcgcg gcgatgatcg
    18121 cgcgcggctt gccgatcaag cacgcagtcg ccatcgatct gcggggccac ggcgacagcg
    18181 agtggccgag cacaagccgt ggttaccaac acgaggattt tctcactgat ttagccggtc
    18241 tcctgcgcca tctgaataaa gagccgctaa ccatcatcgg tcattcactc ggcggcagta
    18301 tgtgcctgtt gtatgcggga accttccctg acaaggtcaa gcgcatggtg ctgctcgagt
    18361 cgctgggtcc atttgcgcgc gccgatgagg aagtgccgga gatttttgcc gagcgactga
    18421 agggtcgaga ctacgtagaa atcccgtttc cacacgaatc gttggaagca gccgcaacgg
    18481 cgctgcgaaa aacttacccg ctcattccgg ataatgccgc gttgcatatg gcacgctacg
    18541 gaaccagtct caaggggggt agataccgtt ggaaatacga tccgattcta cgttatcgca
    18601 ccaccaccgc attgtccgaa ggacagattg aagccttcat ccggcgcgtt cagtgtccga
    18661 tcttctttgt ctatggcacc gagagcgatt tcatgaagtc agtgcgcggt ccgcgggtga
    18721 agctttttcc caacgctgag attgtcccgg ttgagggagc cggccatcac atcccgcatg
    18781 aaaggccgga ggaattagcc gagatcgtcg tgccgtttct gcttcgagct tagcgtgctc
    18841 tttcggtaaa tacgcacaag atgtaactca aggcttacgc gggcaaacta cgatgcattt
    18901 ggaggtaaat gatggcggag ttaagaacaa tcttgcaaaa aatcgatgag gaagccggag
    18961 tagcctggct caccttcaat cgaccggaga agaaaaatgc tttgagccag cggctgatga
    19021 tcgaaatggt tgaggtgtta aaagagctcg gcgacaacga caaaatcaaa tgtatcgtta
    19081 ccaccggttc gggtgattcc tattcatcgg gcctcgatct t
//
LOCUS       DASUVS010000412         3894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1714306, whole genome shotgun sequence.
ACCESSION   DASUVS010000412 DASUVS010000000
VERSION     DASUVS010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714306"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="VF182_22040"
     CDS             complement(<1..1443)
                     /locus_tag="VF182_22040"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6439814.1"
                     /translation="MNTGSYIDGKWFHPKSERLVRNINPADLSDVIAEFPAATSQDAQ
                     RAIEVAQAAFKSWKRTPGPERGRVLWRAADIARQRADEIARTLTREEGKILKEAKGEV
                     LKGISLLEFYAGEGYRMHGKTLPSEARDTFTYTVRRPLGVVGLIAPWNFPWAIPVWKS
                     APALVAGNAVIFKPAELTPATAALLAEIYEQAGLPPGVFNMVVGSGSVVGEAIVNSPT
                     IRAVSFTGSNEIGGALYVKAAQRGAKVTCEMGGKNAVIVMADADLDKAAVAIHGGAFG
                     STGQRCTATSRVIANPAIKEALTERLIEAAKKIKIGSGLDETVDMGPAVDEKQWKTDL
                     DYIKTGQEEGARLLIGGKKPEHLGKGYFVEPTIFDNVAPGMRLFKEEVFGPVLSVSTA
                     KNLEEALSYANGVEYGLTTSIFTENIDTIMNFVEEVETGMVHVNEPTIGGEAQLPFGG
                     TKSTGVGEREMAEEGLNFFTELKTVFINYSG"
     gene            complement(1647..2693)
                     /locus_tag="VF182_22045"
     CDS             complement(1647..2693)
                     /locus_tag="VF182_22045"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6439815.1"
                     /translation="MKFLSRVFILVLSLCSAASAQTGKPMSLAQLAAYNKPDREKVLY
                     AGAKTEGKITWYTSLAGGSYKDLAAAFESQYPGVKIESYRGTRQELGARILAESQAKR
                     HIVDTIETTIPLLKLLKDNKLIAPYYFSTEAKYPEHVKEKGAKGLVYWAIDRESHIGL
                     AYNKNSIPANLVPKNYDGLLRPELKGKIGFAGSDTGVTVVGAMLKFKGEEYFKKLNEQ
                     NPVVHNVSGRALLDMVISGEVGVSPTTFRNHAEVSLKANAPIEWLPMEIVPSNSGSTA
                     VSANAPHPHAALLLADFILGPGGQKVLEQYEYGSPIKDYGFKRWYPEQGLTTEQYEKL
                     DELWNKKLRELARR"
     gene            complement(2879..3808)
                     /locus_tag="VF182_22050"
     CDS             complement(2879..3808)
                     /locus_tag="VF182_22050"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6439816.1"
                     /translation="MGKLLAALAMAWLFTTGAAAPDKMTPVKVTEGVYMLEHSRGSGN
                     STVVITEDGVLVLDFAIENADQTLEFIRKSTDKKIKYLITSHSAGDHASGAWHFREDA
                     PAWIATKNQAHDLQMQEREEFIERRNSKDPRSAAYQKGEPLTPTIAIEGPMTLYFGGL
                     TFQITPEARAHSTGDLTVYIPQKRVMLMGDLLDTEIHPGQGESAGVFFSNVGGWIKVL
                     DNIMARSLAVETYIPGHGPVHIGRGIKDLEEQKHYFVVMRDEVAKMAQAGRTVEQIEK
                     EFKVPQEFAHYKRPERLRQFLKLFYHQLAETGY"
BASE COUNT          787 a         1085 c         1077 g          945 t
ORIGIN      
        1 tcccgaataa ttgataaaca cggtcttcag ctcggtgaag aagttaaggc cttcttcggc
       61 catctcgcgc tcgccgacac cggtggattt ggtgccgccg aagggcagct gcgcttcgcc
      121 gccgatggtc ggttcgttga catgcaccat gccggtttcg acttcttcga cgaagttcat
      181 gatcgtgtcg atgttctcgg taaaaatcga tgtggtcagg ccgtattcca cgccattggc
      241 ataactcaac gcctcttcta gattcttggc ggtggagacc gaaagcacgg ggccgaagac
      301 ttcctctttg aacaggcgca tgccgggtgc gacgttatcg aatatcgtgg gctcgacgaa
      361 ataacccttg ccgaggtgct cgggcttttt accccctatt agcaagcggg cgccttcctc
      421 ctgaccggtt ttgatgtaat ccaaatcggt tttccactgc ttctcatcca cggctggacc
      481 catatcaacg gtttcgtcca aaccggagcc gattttgatt ttcttggcgg cctcgatgag
      541 ccgttcggtg agcgcctctt tgatcgccgg attggcgatg acccgcgatg tcgccgtgca
      601 gcgctgaccc gtggaaccga aggcgccgcc gtgaatcgcc acagccgctt tgtcgagatc
      661 ggcatcagcc atgacgatga ctgcgttttt accgcccatc tcacaggtca ccttggcgcc
      721 gcgctgcgcc gctttaacat agagcgcgcc gccgatttcg ttggagccgg taaaggacac
      781 ggcgcgaata gtcggagaat tgacgatggc ttcgccgaca acggagccgg aacccaccac
      841 catgttaaaa actcccggcg gcaggccggc ctgctcataa atctccgcga gcagcgccgc
      901 cgtggccggt gttagctcgg ccggcttgaa tatcacggca ttgccggcga ccagcgcggg
      961 agcggatttc cagaccggaa tagcccaagg aaagttccat ggggcgatca acccaaccac
     1021 tcccaaaggc ctgcgaaccg tgtaagtgaa ggtatcgcgc gcctccgagg gcagcgtctt
     1081 gccgtgcatg cgataacctt cgcccgcata gaattccaat agtgaaatgc ccttcagcac
     1141 ttcgcctttg gcttccttca aaattttccc ctcctcgcgc gtgagggtgc gggcgatttc
     1201 gtcggcccgc tggcgtgcga tgtccgccgc gcgccagagg actcggccac gctccgggcc
     1261 cggtgttctc ttccagcttt taaaagccgc ttgcgccact tcaatcgccc gctgcgcatc
     1321 ctgtgatgtt gccgccggaa actcggcgat gacgtcgctg agatcggcgg gattgatgtt
     1381 gcgcacaagc cgctcggact ttggatggaa ccacttgccg tcgatataag agccggtgtt
     1441 catgtttgcc tcccgatcag aatctaccta atttgccgca gagcgcgggc aagaagaaac
     1501 aatcagcatg aagaacacga agttgacgag aacatcgaac gatattgatt gcgccttcgt
     1561 gttcttcgtg aacttcgtgg ttgaactttt tcaaggccat agaccgcctg cggttgtgcc
     1621 gagcacgggc ttgaatgcct gtatagttat cggcgagcca gctcgcgcaa tttcttgttc
     1681 cacagctcat cgagtttctc gtactgctcg gtcgtgagtc cctgctcggg ataccaacgc
     1741 ttgaatccat aatctttgat cggactgcca tactcgtatt gctcaagcac tttctggccg
     1801 ccgggaccta aaataaagtc ggccagaagc agagcggcgt gtggatgcgg cgcgttggcc
     1861 gaaactgcgg tggaccccga gttggacggc acaatctcca tcggaagcca ttcgatcgga
     1921 gcattggctt tcagggatac ctcggcatga ttgcggaagg tggtcggcga tacgccgact
     1981 tcgccggata tcaccatgtc gagaagcgct cgtcctgaga cgttatgcac caccggattc
     2041 tgctcgttca acttcttgaa atattcctca cctttgaact tgagcatcgc tccgacgacg
     2101 gttacgcccg tgtcgctgcc ggcgaagccg atcttgccct tcagctcggg ccgcagcaaa
     2161 ccatcatagt tctttggcac caggttggct ggtatcgaat tcttgttata agccagcccg
     2221 atgtgagact cgcgatcgat cgcccagtac accagccctt tggcgccttt ttctttgacg
     2281 tgttcggggt actttgcctc agttgagaaa taatatggcg cgatcagctt attgtctttc
     2341 aagagcttta aaagcgggat tgtggtctca atggtatcga cgatgtgtcg cttggcctgg
     2401 ctctcggcga gaatcctggc gccgagctcc tgccgcgtgc cgcggtagga ttctatttta
     2461 acgccgggat actggctctc gaaagcagcg gcgagatctt tgtacgagcc gccggcgaga
     2521 gatgtgtacc aggtgatctt gccttcggtt ttagcgccgg catacagaac cttttcccga
     2581 tccggtttgt tgtacgccgc cagttgcgcc agcgacatag gcttgcccgt ttgtgccgat
     2641 gcggccgaac atagacttaa aacaagaata aaaaccctac tcaagaattt catgcggtgc
     2701 tcctccgttt cgtcgcggct caaaaagata gctatcgctg aaaagcgctg tttgcaggct
     2761 gctcaaaaag actcagaggc aaggcgcgtg aagtccaatg agctgagacg tacgcttcag
     2821 tacgttgaag cgagtcgggc gaacgcaacg aagcatatgg gtctttttca gctgcctgct
     2881 aatatccggt ttctgccagt tgatgataga acagtttcaa gaattgccgc agccgttcgg
     2941 gacgtttgta atgcgcaaac tcctgaggaa ccttaaattc tttttcgatc tgctcgacgg
     3001 ttctgccggc ttgcgccatc tttgcgactt cgtctcgcat cactacgaaa tagtgtttct
     3061 gctcttcaag atctttaatg ccgcggccga tatgcacggg accgtgcccg ggaatgtagg
     3121 tctcgacggc gaggctgcgc gccatgatgt tatccaggac tttaatccaa ccgcccacgt
     3181 tggagaaaaa tacgccggca gattcgcctt ggcccggatg aatctcggta tcgagaagat
     3241 cgcccatcag catcacgcgt ttttgcggga tatagacggt taagtcaccg gtgctgtgag
     3301 cgcgggcttc cggcgtaatt tgaaaggtaa ggccgccgaa gtaaagcgtc atcgggcctt
     3361 cgatcgcaat tgtcggcgtg agcggttctc ctttttggta agccgcggag cgcgggtctt
     3421 tggaatttct gcgctcgata aactcttcgc gctcctgcat ttgcaaatcg tgcgcttggt
     3481 ttttcgtcgc gatccaggct ggcgcgtctt cgcggaaatg ccaggcgcct gacgcatggt
     3541 cacccgcgct gtgcgatgtg atcagatatt taattttttt gtcggtgctc ttgcggataa
     3601 attcgagggt ttgatcggcg ttctcgatgg cgaaatccaa aaccagcact ccatcctcgg
     3661 tgataacgac cgtggaattg ccggaacccc gagagtgctc gagcatgtaa acgccttcgg
     3721 taactttgac tggcgtcatt ttatccggcg cggccgcccc agtggtaaag agccaagcca
     3781 ttgccagcgc cgccaggagt tttcccattc atgttgtatc tcctcatgag ttattttctc
     3841 ggtgcaagat tgaggcaaag ccgtggattg tgatcgtgtc cgtgaccagc gatc
//
LOCUS       DASUVS010000413         3539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_840151, whole genome shotgun sequence.
ACCESSION   DASUVS010000413 DASUVS010000000
VERSION     DASUVS010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840151"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1202
                     /locus_tag="VF182_22055"
     CDS             87..1202
                     /locus_tag="VF182_22055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6439817.1"
                     /translation="MISVRDLEKSFAGARGEVRALRGVSFEVSKGELFTLLGPSGCGK
                     TTTLRCIAGLEQPLAGEILIDDHPVFSSAGGKFVPPEQRNIGMVFQSYAIWPHMTVFQ
                     NVAYPLAREAGRAEVAERVGRILDRLSLGPLSDRLAPNLSGGQQQRVALARALVAQPQ
                     VLLLDEPLSNLDAKLREQMRFELKALQESFGITTVYVTHDQEEALALSDRIGLMHDGA
                     LIEVGSPADLYLRPAHRLTADFLGTANFIPAEVERDGSGPWDGMLLATSLGSFGAQRS
                     VEWQPGMTAQLFFRPESVEFLDEAVAAADNARNHCTGLIERVTFLGSSADVIIRCSDL
                     KVRARTHPARAPAVGKAVRFRVAQSSCIVFPSRTMNS"
     gene            1296..2246
                     /locus_tag="VF182_22060"
     CDS             1296..2246
                     /locus_tag="VF182_22060"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX6439818.1"
                     /translation="MALSESLNDHERNSRIEWRLYYNMLVTRRFEEALLRWEHQGKIA
                     AQTFPSKGQEAIAIGCCLALEKGDAVIPSFRTRGAMVGMGVSIAEQLREFVHSPLAAG
                     DSRDAPHHSSWPERGVMPGSTMIGGHLAMAVGLALAMQFDRQLTVSVAFFGDGTLGSG
                     DLHESMHIAGAWDLPLIFVCENNGWEMSTPWSKTRKTRSVIPYAEPFGFAARSVDGND
                     ALDVYQAARWARSKALSGRPVFLDCITFRSGLYSSHFGEVRSGIEEDLAEAERRDPLK
                     KMANWLMDHGVATGEDLEKLTQEEDQRLENTFSEVLAELK"
     gene            2339..3295
                     /locus_tag="VF182_22065"
     CDS             2339..3295
                     /locus_tag="VF182_22065"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6439819.1"
                     /translation="MAKITYRDAVGRALQDSMREDSRLIIIGQNLTGHALKSLAETYG
                     ADRVRDCSISESAMVGIAVGAAMKGMKVVVMIAYADIASVCHMAIVQSAAKLHYLSNG
                     RLNCPLIIRLPIARFRGHGPEGNEVGVSWFYNVPDLNIAMPGSANEAYWSFREGLKRS
                     SPTLFFEDRSIHNRSGEIAAESPAGKATVTRPGERLSIIAAGRTAALAEDAADELAAQ
                     AGPNAVEVVSLGFIKPLDHETVLQSVSKTGRVLIVQDEPPYSGYALYVRSLLDNLPAG
                     RLKFAPRIINGAEHFLPFYDERPFLPSLSNVIVTAKELMQSA"
BASE COUNT          769 a          931 c         1055 g          784 t
ORIGIN      
        1 tctcttggcg agcttggcac ctggattgat tgttgtcgga tgattgctcc cgtcaagttg
       61 caaacgccgc aaagattcgg aacaagatga tcagtgttag agatctggaa aagtcgtttg
      121 ctggcgccag aggtgaagtg cgcgcgctgc ggggtgtttc tttcgaagta agcaaaggcg
      181 aattgtttac cctgctcggt ccaagcggct gcggcaaaac gaccacgctt cgctgcatcg
      241 ccggattgga acagccgctc gccggtgaaa ttttgatcga tgatcatccg gttttcagca
      301 gcgccggcgg aaaatttgtt ccacccgaac agcgcaatat cggcatggtt ttccaatcct
      361 atgccatctg gccgcacatg acggttttcc aaaatgtcgc ctatcctctg gccagagagg
      421 cgggccgcgc cgaggtagcc gaacgcgttg gcaggattct tgaccgattg agtcttgggc
      481 cgctgtccga tcggctggcg ccaaacttga gcggcggcca gcaacagcgc gtggcgctgg
      541 cgcgcgcttt agtggcgcaa ccgcaggtgc tgctcctgga cgagccgctc agcaatctcg
      601 acgccaagct acgcgagcag atgcgtttcg agctcaaggc tttgcaggaa tcattcggca
      661 tcacaacggt gtacgtcacc cacgatcagg aagaagcgct cgcgctctct gatcgcatcg
      721 gcctcatgca cgacggcgcg ctgatcgagg tcggttcacc cgccgatctt tatttgcgcc
      781 ccgcgcatcg acttacggcg gattttctgg gcacagcgaa ttttatcccg gcagaagttg
      841 aacgcgatgg cagcggaccg tgggatggaa tgcttctggc gacgtcgctt ggctcgttcg
      901 gcgcgcagcg cagcgtggaa tggcaaccgg gaatgacggc gcagttgttc tttcgtccgg
      961 aatccgtcga gtttctcgat gaagcagttg cggctgcgga taatgcgcgg aatcattgca
     1021 cggggctcat cgagcgtgtc acgtttctcg gcagttcagc agacgtgatc attcgatgta
     1081 gcgacctcaa agtccgcgcg agaactcatc ctgcgcgcgc tcccgcagtg gggaaagccg
     1141 tgcgttttcg cgtagcgcaa agctcgtgta tcgtttttcc gagtcgaacc atgaatagct
     1201 agaatttctt cgcccactaa gtgggagagg caacgtgagg atgtcaacac aacaaacagc
     1261 cgttccatca tcttatctat atttaggagg gagtcgtggc attgtcggaa tcgctcaacg
     1321 atcatgagcg aaatagccgg atcgagtggc gtctctacta taacatgctc gtgacgcgac
     1381 gtttcgaaga agctttgctc cgctgggagc atcaaggcaa gattgcggcg caaacctttc
     1441 cgagcaaggg ccaggaggcg atcgccatcg gttgctgctt ggcgttggag aagggagacg
     1501 cggtgatacc gtcgtttcgc acccgcggcg ccatggtcgg catgggcgtg agtatcgccg
     1561 agcaattgcg ggagttcgtg catagcccgc ttgcggccgg tgattcgcgc gatgcgccgc
     1621 accatagctc atggccggag cgtggtgtca tgccgggttc gaccatgatc ggcggtcacc
     1681 tcgccatggc ggtgggtctg gcgctggcga tgcagtttga caggcaattg acggtaagcg
     1741 tcgcgttctt tggcgacggc acgttgggct cgggagattt acatgagagc atgcatatcg
     1801 ctggagcctg ggaccttccg ctgattttcg tgtgcgagaa caacggctgg gaaatgtcca
     1861 ccccgtggtc gaagactcgc aaaacccgtt cggtaatccc ctatgccgag ccctttggct
     1921 ttgccgcgcg ctccgtggac ggcaacgatg cactggatgt ttatcaggca gcgcgctggg
     1981 cgcgctcaaa ggcactgagc ggtcgcccgg ttttcctgga ctgcattacc tttcgcagcg
     2041 gtctttacag ttctcacttc ggcgaggtgc gatcaggcat cgaagaggat ctcgctgaag
     2101 ccgaacgccg tgatcctttg aagaagatgg cgaattggtt gatggaccac ggagtcgcca
     2161 caggtgagga tctagagaaa ttgacgcagg aagaagacca gagactggaa aatacgttca
     2221 gcgaagtctt agccgaacta aaataagggc gatcgatgag atgtaggggc atccatcgaa
     2281 agcaccttca cgtatgctcg actgcgggtg cgcacaccac gggaggcaag aataaggaat
     2341 ggctaaaatc acctatcgcg atgcggttgg cagagcgttg caggattcca tgcgagagga
     2401 ctcgcggtta atcatcattg gtcaaaatct caccggtcac gcgctgaagt cgttagccga
     2461 aacctacggt gccgaccgcg tgcgtgactg ttcaatcagc gagagcgcca tggtcgggat
     2521 cgcggtgggc gcggcaatga aggggatgaa agtcgtggtg atgatcgcct acgctgacat
     2581 cgcttccgtc tgtcatatgg caattgtcca gagtgcagcc aagctgcact atctcagcaa
     2641 tggacgattg aattgcccgc taatcattcg tttgccgatc gcacgatttc gcggtcatgg
     2701 accggaaggc aacgaagtcg gcgtatcatg gttctacaat gttccagacc tcaacatcgc
     2761 catgccgggt tcggccaacg aggcatactg gagttttcgc gaggggttga aacggtcgag
     2821 tccgacgttg ttcttcgagg acagatcgat ccacaataga tccggtgaaa ttgctgccga
     2881 aagtccggcc ggaaaggcca ctgttacccg gcccggtgag cgcctgagta taatcgccgc
     2941 cggccgaacc gcagcgcttg ctgaagatgc ggcggatgag ttggccgcgc aggcaggacc
     3001 caatgcggtg gaggtcgtga gcctaggttt catcaagccg ttggatcatg agacggttct
     3061 acaatctgta agcaagaccg gcagagtgct tatcgttcaa gacgaaccgc cttacagcgg
     3121 ttacgcgctc tatgtgcgtt ctttactgga taacttgccg gcaggccggc tcaagtttgc
     3181 gccgcgcatt atcaatggtg ccgagcattt tttgccgttt tacgatgaaa gaccattcct
     3241 gccatcattg tcgaacgtta tcgtgaccgc caaagagcta atgcagagcg cctgagtcga
     3301 tttacgcaaa actcgctacc acggcactgg catcaacgct tctaaaagac gcgtcatcgg
     3361 ggcgaaaact ccgaagcagc tccaacgatc ttaatctaag cctcattcca cattctcatc
     3421 tattgtcatt gaaccatcga ttcagttcta acttcgcgag gtcaagcgtt taggtgacgt
     3481 acgaaaatgc tcgagtgaac gataaagtgt ttgatttggt aacggacctt ctaagtctt
//
LOCUS       DASUVS010000414         3543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1932627, whole genome shotgun sequence.
ACCESSION   DASUVS010000414 DASUVS010000000
VERSION     DASUVS010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1932627"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VF182_22070"
     CDS             complement(<1..605)
                     /locus_tag="VF182_22070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6439820.1"
                     /translation="MHKISFGVRVPNSGPLSSVENIVKATKTAEELGFDAIWVHDHVV
                     WSSEMHRHHISSGAAEALTDTQEANFFEATTILSYLAAETKTITLGVACLVMPCRNPI
                     YAAKQYSTLDHLAKGRLLVGVGLGSKATRESDEFGVFGVPYDRRGDRTDEYIEAMKAV
                     WTQPLASYKGDFIQFKDAEVYPKPVQKPHPPVWVGGWMKLAA"
     gene            complement(993..1913)
                     /locus_tag="VF182_22075"
     CDS             complement(993..1913)
                     /locus_tag="VF182_22075"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6439821.1"
                     /translation="MRTEKVNFYSEGNKLAGVLYLPDSDKGKPFAGIVQGPGFLGLKD
                     AKHYIMMFDKLCAAGYACLCFDYRGWGDSEGNDRGWVMPKWQAEDIRNAITFMQTRPE
                     IDPDRIATYGSGGTGGGNAVYVAALDSRVKCCVSYLGVSNGRDWLHSMRREYEWIDYL
                     KSIEEDRKKRVTTGSGAIVSAREGGIMVQTPERQTTNIKKDVADKIPDAMPLACAEAI
                     LEYSPIDVVDKISPRGILFIAVEGDAVTPEQQSFDLYQKAREPKKLVLYKKTTHYGIY
                     NDYFDDVAANIIDWYNRHLKYNKVEITEKE"
     gene            complement(2093..3094)
                     /locus_tag="VF182_22080"
     CDS             complement(2093..3094)
                     /locus_tag="VF182_22080"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6439822.1"
                     /translation="MATKLKFGLLLPHFGEYASAESCIEGSKKAEKYGFDSVWVRDHL
                     VFEPHGMEGNDNTHIEGLLILAAIASVCKKLILGTGTMISHRHPIHLAQAVAGLSTLC
                     DSNIIMGLGLGTFPHEFEAAGYKKVTLQDRANIAKANALLCRRFWAGEKIAEYKDDYY
                     EFKDVELRPTPTKNIPIWYGGGTPASCRRAADYCDGWMPGRIPTATFNKMVKYLEEQC
                     KLAGRPMVTTGAIPITSIDKNRDVALSKVNTKGLINEGNSPSKKTWVKPKSGTFSTVE
                     DIEGLLMAGTPADIARDTKRYEEAGLNHIVFDLRFRYADWYEQIDYLGQEVLPKVRA"
     gene            complement(3207..>3543)
                     /locus_tag="VF182_22085"
     CDS             complement(3207..>3543)
                     /locus_tag="VF182_22085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439823.1"
                     /translation="KKFLVTRRDTVRRITMALIEATHFLKTRKEESKKLIGKYARQYN
                     PQFLEASYNAMVKLHDRVPLVTREGTQVQIDEALSRRPGATLKIEDLIDDSVVRELEK
                     SGFIEQIFK"
BASE COUNT          792 a          972 c          876 g          903 t
ORIGIN      
        1 gcggcaagct tcatccagcc gccgacccaa accggcggat gcggtttttg caccggtttg
       61 ggataaacct cggcatcctt gaattggata aagtcaccct tgtatgacgc taatggctgc
      121 gtccacaccg ctttcatcgc ttctatatac tcatcggtgc ggtcgccgcg ccgatcgtaa
      181 ggcacaccga agacgccgaa ctcgtcagat tcacgagtgg ctttcgagcc cagaccgacg
      241 ccgaccaaca gacgaccctt ggccagatga tccaatgtcg aatactgctt tgccgcgtaa
      301 atcggattgc ggcagggcat gaccaggcat gcgacgccaa gtgtaatcgt cttggtctcc
      361 gcggccagat aagagaggat cgtggtcgct tcaaaaaagt ttgcctcctg agtgtcggtc
      421 aatgcttcgg cagcgccgga ggaaatgtga tggcggtgca tctccgagct ccagaccacg
      481 tgatcatgaa cccagatggc atcaaagccc aactcttccg cggttttggt tgctttgaca
      541 atattttcca cgctggaaag cgggcctgag ttcggcaccc gcacgccgaa agaaattttg
      601 tgcatgactt gctcctactt tctactcacg gcttctttgt aaggcagttc cgaaagatcg
      661 aagtgccgca attaaatccc taatctcaat attgaaatcc tacacacaca aagaaatcaa
      721 aatcgaatcg ctttttcgga actgcggaga attatttcgt aatcccaata tcgaaatcgt
      781 aaacatacag aagaaatcaa atcgctttgc cgaaatgcgg agtattattt cataatctga
      841 acgtcgacgt taaacaaaca gcagggaatc aaaatcaatt cgtgaaactc agcggtcgtg
      901 tttgggacgt cttgatcgtt ggaatttgtt tcgatcttcg gatttcgaat ttcggatttc
      961 cagtgcggcc gtcagatgtc ttacgggatc ggctactcct tctcagtgat ctcgactttg
     1021 ttgtatttca ggtgacggtt ataccagtcg atgatgttcg cggccacatc atcgaagtaa
     1081 tcattgtaaa taccgtaatg agtggtcttt ttgtaaagca ccagtttttt cggctcgcgc
     1141 gctttttggt acaaatcaaa cgactgctgc tctggcgtca ccgcatcgcc ttcaacggcg
     1201 atgaacaata tgccgcgcgg cgaaatcttg tcgactacat cgatggggct gtattccaga
     1261 atggcttcgg cgcaggccag cggcatggcg tcgggaattt tatccgcgac atctttcttt
     1321 atattcgtgg tttgacgctc gggagtttgc accatgatgc ctccttcccg cgcgctaacg
     1381 atggcgccag agccggtggt cacccgcttc ttgcgatctt cttcgatgct cttcaagtaa
     1441 tcgatccatt catattcgcg ccgcatcgaa tggagccagt cacgcccatt gctgaccccc
     1501 aggtagctga cgcagcattt gacccggctg tcgagggccg cgacatagac ggcgttgccg
     1561 ccgccggtgc cgccggagcc gtaagtggcg atacggtcag gatcgatttc agggcgtgtt
     1621 tgcataaagg tgatggcatt gcgaatgtct tcggcctgcc atttgggcat tacccagccg
     1681 cgatcgttac cttcgctgtc gccccagccg cggtaatcga agcataagca ggcatagccg
     1741 gccgcgcaca gcttatcgaa catcatgatg tagtgcttgg catctttgag cccgagaaat
     1801 ccgggcccct ggacaatgcc ggcaaatggt ttgcctttat ccgaatccgg caaatagagg
     1861 acgcccgcga gcttgttgcc ttcactgtag aaattgacct tctctgtacg catctcaaac
     1921 tcctataaca gccagttgaa aaaggctcat ctgcttcgtt gcgctcgatc gcttcgctcc
     1981 aacgtacctg agtacgtctc cgctcatcga tttcccgcac gcctcgcagc taaacctttt
     2041 tgaacggcct gaaaatattt cagacaccca aaccccgcac ccgaaaccta cgttatgccc
     2101 gcaccttggg tagaacttcc tggcccaaat aatcgatctg ctcgtaccaa tcggcgtaac
     2161 ggaatcgcag atcgaaaacg atatgattca aaccggcttc ttcgtatcgt ttagtgtcac
     2221 gggcaatatc cgccggagtt cccgccatga gcagcccttc gatatcctcg acggtggaga
     2281 aggttccgga tttcggtttg acccaggttt ttttactcgg ggaattgccc tcgttgatta
     2341 gtcccttggt gttcactttg ctgagagcaa cgtcgcggtt cttatcgata ctggtaatcg
     2401 ggattgcgcc ggtggttacc atggggcgtc ccgccagctt gcactgctct tcgaggtact
     2461 tgaccatttt gttaaaagta gccgtgggaa tgcgtccagg catccagccg tcacagtaat
     2521 ccgccgcgcg acggcaggag gccggcgtgc cgccaccgta ccagatcgga atatttttag
     2581 tcggcgtcgg tctgagctca acgtctttga actcgtaata gtcatccttg tactcggcga
     2641 tcttttcacc ggcccagaaa cggcggcata agagcgcgtt ggccttggcg atgttggcgc
     2701 gatcctgcaa cgtaactttc ttataacccg ccgcctcgaa ttcgtgagga aaagtgccga
     2761 ggccaaggcc catgataata ttgctgtcgc acagtgtgct caggccggcg acggcctgcg
     2821 ccaaatgaat cggatgccgg tgcgagatca tcgtgccggt gccgagaatg agtttcttgc
     2881 acaccgacgc gatcgccgcc agtatcaaca gcccctcgat atgggtgtta tcgtttccct
     2941 ccatgccgtg cggttcaaaa accaggtggt ctctgaccca aaccgagtcg aagccatact
     3001 tttccgcttt cttcgagccc tcgatacagc tttccgccga cgcatattcg ccgaaatgag
     3061 gtaaaagcaa tccgaacttg agttttgtcg ccatgtctct tctccttctg tcgcttcgtc
     3121 gttaaaaaaa atgacttagc atattaatcg cggcattgtc tatgacgcgg cggacggttt
     3181 aaaccgggcg aaagaggttg agtcgtttac ttaaaaatct gctcgatgaa accgctcttt
     3241 tctaattccc tgacaacgct gtcatcaatc agatcttcaa tctttagcgt ggcccccggt
     3301 ctgcgactca gcgcttcgtc gatctgaact tgcgtgcctt cgcgcgttac caagggaaca
     3361 cgatcatgca acttgaccat ggcattgtag gaagcttcca agaactgagg attatactgc
     3421 cgcgcatact tgccgattaa cttcttgctt tcttcctttc gggtttttag aaaatgcgtc
     3481 gcctcaatca gcgccatcgt aattcgtcta accgtatcac gtcgggtcac cagaaatttc
     3541 ttg
//
LOCUS       DASUVS010000415         6099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1056715, whole genome shotgun sequence.
ACCESSION   DASUVS010000415 DASUVS010000000
VERSION     DASUVS010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056715"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VF182_22090"
     CDS             <1..268
                     /locus_tag="VF182_22090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439824.1"
                     /translation="TAAKKDRCGFTHHQRLILCYDSLSHTTDRYQLRLMIRCILERDP
                     DYGDERNRDFHGHLRRENLANFSNLLAFDMIQSGGLPDRLGLLV"
     gene            282..1931
                     /locus_tag="VF182_22095"
     CDS             282..1931
                     /locus_tag="VF182_22095"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6439825.1"
                     /translation="MYNEYFGFSEAPFSLTPDPRFSYTNALYQEAFATLRYGIEARKG
                     FIVITGEAGTGKTTLLRRLMQSFGRHVQTAYIYNPHVTLTELLRLILSDLGLANRTDD
                     RLAMIAQLNEYLIKQLKERNIVTLLVDEAQELSVEMLEEVRLLSNLETDTQKLLQIVL
                     MGQPELERKLDEPELRQLKQRVALRCRLDPLPSNEVGEYISARLKAVGYSRRDLFHAS
                     AVEKIAGHSRGIPRIINIICDNALLIAFAASKKSVSAATIDEVARDLRLNQQSATPEI
                     TPAPPVQKGSIENLEGFRSTRASSRSGARTPASPESEFFMIGLEEKPANLRGEKKLAG
                     YGTGVFSLGIVLGLAAMLFYSQQMWGGLAALTESWNGFNAPKTSDSMPAQPTVPDGRS
                     TSDLGNEKRVLESAPPDFSGMEQQTLTESPSPNITEQSQEQSLQPAAPVEQPRSATTG
                     RRAPDAARGERRTPLPATPSPAALPRNETVTDRELEMQVEKAIDNRALRGIGVSVKDG
                     TVYLRGRVPTTRQRLAAQRAAQSVDGVKSIENQIGLDTGLD"
     gene            complement(1998..3008)
                     /locus_tag="VF182_22100"
     CDS             complement(1998..3008)
                     /locus_tag="VF182_22100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439826.1"
                     /translation="MPAKPHSIIVLLLLLTVFVGCRTGAVPPAPPAAEQAPQTGTLET
                     SDESGPEPAIAALGIPRITPAPPQRVLPESVLSAPDKESVGPAIQYDTVLTKRAIHTD
                     ANSGWQYRNNRQRQIVGFEFSNNGGNSILPQRRDISKNQLYTRDFQFRFDDRARQDMH
                     LSVSDWVASRDKQFRLSELMNSVMLFFPRNYLPAVVTIGDRDIVTLPTGEEVEFDAHT
                     HEVNGGVFSEAPVDLNPDKNLRQFPAIRYTGSGVMVRADARGLDPRLGRVAIVTTGSP
                     AKDCIQGRACNQCEVPAKEIWAQSGAARFKFAHDEDFHNYLLSRCAFGLPMKIGSVIG
                     SR"
     gene            complement(3207..3785)
                     /locus_tag="VF182_22105"
     CDS             complement(3207..3785)
                     /locus_tag="VF182_22105"
                     /inference="COORDINATES: protein motif:HMM:NF021141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2459 domain-containing protein"
                     /protein_id="HEX6439827.1"
                     /translation="MQVASHVPGENAKRVYVVHNNWHTALVVRKADLIHGLLPERDDF
                     PEAEYLEIGWGDRDYFPAIEESVGLALRAAFWSRGSVLHVVGFKGAVTDYVTDGEIIE
                     LALSREAFLRLSAFLSASFSRTDPSVPAPSQPGLVATGRFYPATGRFSILRTCNTWIA
                     QSLKSAGLDITPLYVITAASLARQVRPYRVMN"
     gene            4137..5417
                     /locus_tag="VF182_22110"
     CDS             4137..5417
                     /locus_tag="VF182_22110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6439828.1"
                     /translation="MNLKNTSAPEDSAAPNKVAFAALHHRDFRAYFIGTMLAMMADNI
                     EHVISYWLLYQKFQSPTLAGFAVLVHWTPFLLFSVYFGALADRYDCRKVIQLAQLMYM
                     SVSAGWAFLFWTDTIEVWHACVLLTIHGMAGVLWGPGSQLLIHDIVGTEHLQSAVRLN
                     ATSRQLGVLFGPAIGGALMLLLGPPAGLFVNALIYLPLTIWLWTVPYTGHSRQAAALP
                     RRSVGWRDALDTLREVSGNRPIITMVALGGCASFFVGSAFQAQMPEFAHDLGTDKADF
                     AYSALLAANAAGAVVGGFLLEGKGWLPPRVKTAIVCAILWCLVITAFALSYNYNLSLM
                     LLFFAGLLNLAFYSMAQTIVQLLAPNHLRGRVIGLFSMASMGLRAFSGVTVGVVGGLI
                     GVHWSLALSAMALFAVTLGLFAFFMPGKHQGEFG"
     gene            5511..>6099
                     /locus_tag="VF182_22115"
     CDS             5511..>6099
                     /locus_tag="VF182_22115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6439829.1"
                     /translation="MRGQQKSPISAELNKKVAFAALHHRDYRRYFLVNLFSTMGDNIE
                     HVISYWLLFQKFHSPVLGGFAVISHWTPFLFFAVYFGALADRHDCRKVIQASQFLYAT
                     VSLTWAILFLTDTIEIWHACVLLVIHGMAGVLGGPSSQLIIHDIVGREYLQSAVRLNS
                     TARQLGILFGPAVGGATMLLFGAPAGLFINTLMFLP"
BASE COUNT         1288 a         1644 c         1723 g         1444 t
ORIGIN      
        1 aacggccgca aaaaaagacc gttgcgggtt cacccatcat cagcggttga tcctctgcta
       61 tgactcactg tcccatacta ctgatcggta ccagctgcgt ctaatgatca ggtgcatctt
      121 agagcgcgat ccggattatg gcgacgagcg caaccgtgat tttcacggac atctgcgcag
      181 ggaaaacctg gcgaattttt ctaacctgct ggcttttgat atgatacaaa gcggtgggtt
      241 gcccgacagg ttaggcttgt tagtctaatt ggcagtggtt tatgtataac gaatatttcg
      301 gcttcagcga ggccccattt agcctgacac cggatcctcg gttcagttat accaacgcgc
      361 tttatcagga agcgttcgct actttgcgct acggcattga ggcgcgcaag ggttttatcg
      421 tcattacagg cgaagcgggc acgggcaaaa cgacgcttct gagacgttta atgcagagct
      481 ttggccggca cgtgcaaacc gcctatatct ataacccgca tgtgactctc acagagctgt
      541 tgcgtctgat tctgagcgat ctcggtttgg cgaaccgcac ggatgaccgc ttggcgatga
      601 ttgcgcagtt gaatgaatac ctgatcaagc agctgaaaga gcgtaatatt gttactcttt
      661 tggtcgacga agcccaagag ctcagcgttg agatgctcga ggaagttaga ttgctctcga
      721 atctcgagac cgacacgcag aagctgctgc aaattgtttt gatggggcag cctgagctcg
      781 agcgcaagct tgacgagccg gagctgcggc agttgaaaca gcgggttgcg ctacgttgcc
      841 ggctcgatcc tcttcccagt aatgaagtcg gtgagtacat ttcagccaga ctcaaagcag
      901 ttggatactc gcggagagac ctgttccacg cttcagctgt ggagaaaatc gccggccatt
      961 cgagaggtat cccccgcatc ataaacatca tctgcgataa tgctctactg atcgctttcg
     1021 cggcctccaa aaaatctgta tctgcggcga ccatcgatga ggtcgcccgc gacttgcgtc
     1081 tcaaccagca atctgccact ccggagatca cgcccgcgcc gcctgtgcaa aaaggttcaa
     1141 ttgaaaatct agaaggattt cgttccacac gcgcctcaag ccgttccggc gcgcgcacgc
     1201 cagcctcgcc ggaatccgag tttttcatga tcggtcttga ggaaaaaccc gccaatcttc
     1261 gaggcgaaaa aaaactggct ggatatggaa ccggggtctt ttcgctgggc atcgttctag
     1321 gacttgccgc catgttgttc tattcccaac agatgtgggg cggtttagcg gccctgactg
     1381 agagctggaa tgggttcaac gctcccaaaa caagcgattc tatgccggcg cagccgacgg
     1441 ttcctgacgg tagatcaaca tccgatctag ggaacgagaa gagggttctt gagtctgcgc
     1501 cgccggattt ttccggaatg gagcagcaaa cgttgactga gagtcctagc cctaacataa
     1561 ccgagcagtc gcaggagcaa tccctccaac ccgccgctcc ggttgaacaa ccgcggagcg
     1621 cgacaacagg tcgaagggcg ccggatgctg cgcggggcga gagaagaact cctctcccag
     1681 ccacgccatc tccggctgcg ttgccgcgca acgagacggt tacagaccgc gagttggaga
     1741 tgcaggttga gaaggcgatc gacaatcgcg ctttgagagg gatcggcgtg tcggtaaagg
     1801 acggcacggt atatctcagg gggcgtgtcc ctacaacgcg ccagagactt gcggcgcagc
     1861 gcgcagcgca aagcgtcgac ggcgttaaat ctatcgagaa tcagatcggg ctcgacaccg
     1921 ggctggatta agccgcgttg ctgtgccgtc tacggagcat cgagctaaag agttcgacgg
     1981 gcagccaacg atgtgatcta gcgtgatccg attacagaac cgattttcat gggaagccca
     2041 aaggcgcaac gcgatagtag atagttgtgg aaatcctcat cgtgggcgaa tttaaaccga
     2101 gcggcgccgc tctgcgccca aatttctttg gccggaactt cgcattgatt gcaggctctg
     2161 ccctgaatgc agtctttggc aggtgatccg gtcgtgacga tcgccactcg tccaagtctg
     2221 ggatccaagc cacgcgcatc ggcgcgaacc attacgcctg aaccggtata acgaatagcc
     2281 ggaaattggc gtaaattttt atctggattg agatccacgg gcgcttccga aaacacgcca
     2341 ccgttgactt catgcgtgtg cgcgtcgaac tccacttctt cgccggtggg cagggttaca
     2401 atgtcgcgat cgcctatcgt gaccacagcc ggtagatagt tcctggggaa aaacagcatg
     2461 acgctgttca tcagctcgct aagtctaaac tgcttatcac gggaagcgac ccagtcacta
     2521 accgatagat gcatatcttg ccgggcacga tcatcgaagc ggaattgaaa atccctggtg
     2581 tagagttgat tcttgctgat atcgcgacgc tggggaagta ttgaattgcc gccgttattg
     2641 gagaactcaa agccgacaat ttgccgttga cggttgttgc gatactgcca gcctgagttc
     2701 gcgtccgtat gaatcgctcg ttttgtcaga accgtgtcgt attggatcgc cggccccacg
     2761 ctttctttgt cgggggcgct gagaaccgac tcaggcaaaa ctctttgtgg aggtgccggc
     2821 gtgatccgtg gaattccaag cgctgcgata gccggttccg gtccagattc gtcagacgtc
     2881 tcgagagttc ccgtctgtgg cgcttgctcg gcggcgggcg gcgctggagg cactgcaccg
     2941 gttcgacaac ccacaaaaac cgtgagcagc aaaagcaaaa ctatgatcga atgaggtttt
     3001 gccggcatgg tccgcttccc aagatctcca agaggctctg agaccaagag gctctgagac
     3061 caaatagact gcgatcccag atccatcgca ctttcaccgg gctattttaa cccaggcccg
     3121 tgctggtgcc aaggtcgttc aagctaacgg tgtctcggct cgcggccagc gccggggaaa
     3181 actgcattct atgcgctagg gaataactaa ttcatcacgc gatagggccg gacctgccga
     3241 gccaggctgg cagcggtgat gacgtagaga ggagtgatat caagtccggc gctttttagg
     3301 gactgcgcta tccaggtatt gcacgttctc agaatgctga atctgccggt tgccggataa
     3361 aaccggccag tcgcaaccag tccgggctgt gacggggccg gaaccgacgg atccgttcta
     3421 gagaagctcg cggataagaa tgcgctcagc cttaggaagg cttctcgcga gagagcgagt
     3481 tctatgatct caccgtcggt aacgtaatct gtcaccgcgc ccttaaagcc gaccacatga
     3541 agtacgctcc cgcgcgacca gaatgccgcc ctaagcgcca atccaacact ttcctctatc
     3601 gccgggaagt aatctcgatc tccccaacct atttcgagat actctgcttc gggaaagtca
     3661 tcccgttccg gaaggaggcc gtggatcaga tcggcctttc gcacgactag cgcggtatgc
     3721 caattgttgt gtacgacata gactcttttg gcgttttctc cgggcacgtg agaagctact
     3781 tgcatgatcg gcattgaaca ggcagtcagt aactggagta aagcagcaaa caaaacaaat
     3841 ttcatccaga gcaacgattt taccgtgaca tattactgat tggggcgaaa tcagcatgag
     3901 ccgcgttgcg tctaaaggat cgaggcggag ctacggtttg cataggtttt taatatccat
     3961 tactgtcgga ttcagcaggc tgctcaaagt gatttaccgg ccaaattgcg ctgattaacg
     4021 caagctgatc gttgtcgaaa ccactcttag cgatgactcg tatagatttc tttgacctgc
     4081 ctgtcacggc gctttcaaag cgctgatccc tagtttccaa gtcacggagc tgcgatttga
     4141 atttgaagaa cacctctgcc ccggaggatt cggcggcgcc caacaaggtt gcctttgccg
     4201 cgcttcatca cagggatttt cgggcttact ttatcggcac catgctggcg atgatggccg
     4261 ataatatcga gcatgtcatc agttactggc tgctgtatca gaaatttcag tcgccgacct
     4321 tggccggttt cgccgtgctg gttcactgga ctccgtttct cttgttctcg gtttattttg
     4381 gcgcgctggc ggatcgctat gattgccgca aagtgattca gctggcgcag ctcatgtata
     4441 tgagcgtgtc ggccggatgg gcgtttctgt tctggaccga taccatcgag gtttggcatg
     4501 cctgcgtgct gctgactatc catggtatgg ccggcgtgct ctgggggccc ggcagtcagc
     4561 tattgatcca cgacatcgtc ggcaccgagc acttacagag cgccgtgcgc ctcaatgcca
     4621 ccagccgtca attgggggtg cttttcggcc ccgccatcgg cggcgccttg atgctgctgc
     4681 ttggacctcc ggcggggctg ttcgttaacg cccttatcta tttgccgttg acgatctggt
     4741 tgtggaccgt gccttacacc ggccattctc gacaagctgc ggcgctgccg cggcgcagtg
     4801 tcggttggcg tgacgctctc gataccttgc gcgaagtatc gggcaaccgg ccaatcatca
     4861 cgatggtcgc tctcggcggt tgtgcatcgt ttttcgtcgg cagcgcattt caagcgcaga
     4921 tgccggaatt cgcccatgac ctgggcacgg acaaagcaga cttcgcgtac agcgccttac
     4981 tggcggccaa tgccgcgggc gccgttgtcg gcggattttt gctggaagga aaaggatggc
     5041 tgccgccccg ggtcaaaacc gcgatcgtct gcgctattct ctggtgtctc gtcatcaccg
     5101 cgtttgcgct ttcgtataat tacaatctgt cgctgatgct gttgtttttc gccgggctcc
     5161 tcaatctcgc cttctactcc atggctcaaa ctatcgtgca actgctcgcg ccgaaccacc
     5221 tccgtggccg cgtcatcggt ttgttttcga tggccagtat gggcttgcgc gcgtttagcg
     5281 gtgtgacagt gggtgtggtc ggtggtttga tcggcgttca ttggtcgctg gcgttgagcg
     5341 cgatggcgtt gttcgcggtg acgttagggc tgtttgcctt tttcatgccc ggcaagcacc
     5401 agggcgagtt tggctgaagg ttgacggagt gatgctcgtt ccgcgaactc ggtgcggtga
     5461 tcaggactaa actgaatgtg aagcaaatca gtcttcggag ttccttttag gtgagaggtc
     5521 agcagaaatc tccgatatca gccgagctga ataagaaagt cgcatttgcg gcgctgcatc
     5581 atcgcgacta ccggcgatat tttctcgtta acctgttctc gaccatgggt gacaacatcg
     5641 agcacgtgat cagttactgg ctgctgtttc aaaaatttca ttcgccggtt ctcggcgggt
     5701 tcgccgtaat cagtcactgg acaccatttc tttttttcgc ggtttacttc ggcgcgctgg
     5761 cagaccgcca tgattgtcgg aaagtcattc aagcatctca gttcctctat gcgacggtgt
     5821 cgctgacttg ggcgatcttg tttctcaccg acaccatcga gatctggcac gcctgtgtgt
     5881 tgctggtgat ccacggcatg gctggagtgc tgggcgggcc gagcagccag ttaattattc
     5941 atgacatcgt cggtcgcgag tacctgcaga gcgcggttag actcaattcc accgcgcgtc
     6001 aactgggcat tctcttcggc cccgcggtgg gcggcgcaac catgttgctg ttcggcgcgc
     6061 cggcaggact attcatcaac acgttgatgt tcctgccga
//
LOCUS       DASUVS010000416         9988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1278185, whole genome shotgun sequence.
ACCESSION   DASUVS010000416 DASUVS010000000
VERSION     DASUVS010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9988
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278185"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..4178
                     /locus_tag="VF182_22120"
     CDS             36..4178
                     /locus_tag="VF182_22120"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6439830.1"
                     /translation="MLPGRETTAGRVALEHRVVHIHDTLADPDYFREAHRLGGQRTVL
                     GVPMLREGTLIGVIIIRRTQVQPFTDKQIELVTTFADQAVIAIENVRLLQELQNRNQA
                     LTEALDQQTATSEILGVIASSPTDIQPVLDVVAESAARLCEASDALIIRAEGENLKAA
                     AHYGPVPSLMDWCYPLSRGFPGGRAVIDQQPIHVHDIAAAIETEFPEVPFVHQHSGAR
                     TILATPLLREGLGIGAIVIRRQEIRPFSEKQIALLKTFADQAVIAIENVRLFKELGER
                     NADLREALEHQTATAEVLGIISRSPTDIQPVLDAIVESAARVCGIDNLGLRLREGNNL
                     VSRAQFGPVPPGRVEVSVDEPAPRWVREHGTLHVPDVSKQNDVQITNSSGSRSYLFVP
                     LRQKGEFIGALSARRKEVRPFTPAQIKLLETFADQAVIAIENVRLFQELKESLEQQTA
                     TSEILGVIASSPTDVQPVLDTIAESAARLCSADDSSIRIVEGDILRLMTHKGSIPFFM
                     TRELPITRGSVAGRAVVDDELIHIEDLTLRGTEFPDVQVALDREGVRTILVAPLEREG
                     RAIGVILIRRTEVRPFSEKQIALLKTFADQAVIAIENVRLFKELQARNRDLTEALEQQ
                     TATSEVLKVISRSTFDLEPVLQTLLENATRLCGAAHGSMHRVEGDVIPVMATYGQSQE
                     LQEFLKEHPARIGKKSVVGRVVMQRQAVHIPDLRADPEYDYAAKFFEPRSVLGVPLLR
                     EGLPIGVIIIFRTEVDPFTEKQIDLVTTFADQAVIAIENVRLLQELQSRNRELTEALE
                     QQTATSDVLNVIAHSPIALERVYEAIVANTTRLCEANIASLFLYDGEVLSTAASHGTT
                     QEFAEHLEHSRPRPSRETTTRLAALEHRTVHVSDLLSDRAFSPQPRQLYEKENVRTVL
                     SVPMLREDKLIGVITTWRREVRPFSDKQIALVKTFADQAVIAVENVRLFQEIQERTRE
                     LQLSLEEVGALSEVSRAVSSSLNLHEVLSRVAGHAVNLSKSDGCGIFEFNPVRHAFDV
                     VASHNLSREFLESVQTTTIDLGNTTIGQAADRAQPIQVPDMTNAYGHPFRDFTLKAGF
                     LSVLTVPMRGNGVTRGIVLLRRSPGSFDDRVVNLLTALASQSKVAIENARLFSEIEDK
                     GRQIEAANRHKSEFLANMSHELRTPLNAIIGFSEVLLDPALRVSEEERMQFLTDVLGS
                     GKHLLGLINEVLDLAKIEAGKMELQIDRALMQDVVEAVANTMRSLATKKGIQLRIHSD
                     ERLAPFSMDGARVKQVLLNLVGNAIKFTPEGGKVWIRTGGKNGFVCVEVGDNGPGIAA
                     EDQERIFLEFQQAGSDAGKPQGTGLGLALAKKFVEMHGGTIWLESEVGKGSRFFFTLP
                     TN"
     gene            4330..4698
                     /locus_tag="VF182_22125"
     CDS             4330..4698
                     /locus_tag="VF182_22125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6439831.1"
                     /translation="MNEKKILLVEDNPVNRRLAGFLLRSQGYEVVEATNAAEALQILA
                     KDPPDLIVMDIQLPEMDGLEATTKLKAEPATAKIPVIAVTSYAMAGDRERAIAAGCSG
                     YVTKPIDKTVFLAEISRHLK"
     gene            4710..6080
                     /locus_tag="VF182_22130"
     CDS             4710..6080
                     /locus_tag="VF182_22130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6439832.1"
                     /translation="MDSATLTPAGARVLIVDDDGASRRLLRVRLEAMECTVAAVADGP
                     EALAEIRKHEPELMLLDLQMPKMGGIEVLKELAKQGAKFPVVVISAHGSIESAVDAMK
                     QGAYDFITKPIDAKHFEIVVRKALEREGLKRGLELLTEETDSRHRLIVGQSKRMAEAI
                     ETARKAADSKATVLLLGESGTGKEIFARAIHNWSDRKSQPFVVINCVGLSKELLESEL
                     FGHEKGAFTGADQQKKGKMELANGGTVFLDEIGDMSQELQTKLLRFLQEREFDRVGGV
                     RPIHVDVRIICATNRDLETEVKDGRFRQDLYHRINVIPITLPPLRERVEDIPVLAHHF
                     VEYYAKEAKKNFTEIVADALKQLCAYDWPGNVRELGNAIERAIVLGHGPYVTVQDLPS
                     RVAASVVRPTIDGRSYRDAMEDYRKELILVALERCRGNRAAAARLLGLHEKYFLRLLK
                     SLAISV"
     gene            6319..6801
                     /locus_tag="VF182_22135"
     CDS             6319..6801
                     /locus_tag="VF182_22135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439833.1"
                     /translation="MAIKILVPLRKHDRLDEIVPYIEEVAEPGASIVFLIRHPVSGFK
                     WLQAYAAISQCGIETALAIRRMAESYSTRTRMQLAGQKVFSACEALHRLGVTVTVDIY
                     ASSLSKALSNYASHENVQFVVMQPGIGQRIARYLQGVISIQSIFRHPFSLSIFIRPSA
                     "
     gene            6871..8112
                     /locus_tag="VF182_22140"
     CDS             6871..8112
                     /locus_tag="VF182_22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005560157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX6439834.1"
                     /translation="MLCRTAPGTPMGELFRRFWLPVALSRELPKPDCAPVRLRILYED
                     LIAFRDTNGKVGLLARYCPHRGASLFFGRNEGGGLRCVYHGWKFDVTGACVDMPNEPA
                     ECSFKHKIHQIAYPTCEAGGVIWAYMGPQASKPEMPRLEWTLVPQSHVYAHKRFQHCN
                     YLQNLEGEVDSSHVSFLHREFRPEKFNAAIAGQTLLATGKDSAPKFLVEDTEYGLAIG
                     ARRNWDGEQYYWRLTQFLMPSFTLIPSEAGSPINFTAAVPVDETTMVGFTVTWLPDRA
                     LNQDDIRTIETWTGAYAEVDPDTFEPVANRQNHYLLNREKQRNENFTGMRGIREEDIA
                     IQEDQYGPIADRTKEHLGSSDAGIIALRRRLLKSVRDLQAGREPPELHCGATYRVHPT
                     ASLAPRAISFEEVTRSAIPAV"
     gene            8329..>9988
                     /locus_tag="VF182_22145"
     CDS             8329..>9988
                     /locus_tag="VF182_22145"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6439835.1"
                     /translation="MKQRTAPSGDSTSTLKKTIAAQAQEIRERAEQQAATSAILRTIA
                     RAPADLQAVFDGIAEHAAKLCDAADALVWRVNGTARYLAAHFGSVPTSGDIGTEEYEL
                     ARTGPPGRAILDRETVHVHDLRASSADFPGAKTRGVAFGIRTTLVTPLLRDGMAVGAL
                     QIRRKEVRPFTDAQIKLLETFADQAVIAIENARLIHEQQASNRELTEALQQQTATSEI
                     LRVIASSPTDIQPVLDAVAESAARLCESYDAQIFRLENEVVHRVASYGPLLIAMEHTP
                     LNRQSPAGRAMVDRQAIHVHDLAAEIDSEYPEIRGYQQQIGHRTTLAAPLLREGVPIG
                     AILIRRLEVRPFTEKQIALLKTFADQAVIAIENVRLFQELGQRNAELREALEHQTATS
                     EVLGIISRSPTDVQPVLDAIVESAARLCESYDVQIFRLENEVVHRVASYGPLLIAMER
                     TPLNRQSPAGRAMIDRRPVHVHDLAAEINSEYPEIKGYQEQIGHRTNLAAPLLREGAP
                     IGAILIRRLEVRPFTEKQIALLKTFADQAVIAIENVRLFQELGQR"
BASE COUNT         2306 a         2665 c         2900 g         2117 t
ORIGIN      
        1 cccgggagtg gatagatttc attgagaaga acccaattct tccaggacga gagactacgg
       61 caggccgggt ggcgctcgaa cacagagttg ttcacattca cgataccctc gcggaccccg
      121 attattttcg tgaagctcac agactcggcg gtcagcggac tgttctagga gtgcccatgc
      181 ttagggaggg cactctcatt ggagtgatta taatccgcag gacccaggtc cagcctttta
      241 ctgataagca aattgaactg gtgacgactt tcgctgacca agcagtaatc gcaatcgaga
      301 acgtgcgctt gctccaagag ctccaaaacc gcaaccaagc cctcaccgaa gctctcgatc
      361 agcagaccgc gaccagcgaa atactcggcg ttattgccag ctcgccgacg gatattcagc
      421 cggtgctcga tgtggtcgcc gagagtgccg cgcgcctatg cgaggcaagc gacgctctga
      481 ttatccgtgc cgaaggcgaa aacctaaaag cagcagcgca ctacggtccg gtgccgtccc
      541 tgatggattg gtgctatcca ctgagtcgtg gatttccagg cggacgggct gtcatcgacc
      601 agcagccgat tcatgtccat gatatcgcag ctgcaatcga aaccgagttc ccagaggttc
      661 ccttcgtcca tcagcactcg ggtgctcgga caatcctggc caccccttta ttgagggaag
      721 gtcttgggat cggagctatt gtgatccgac ggcaagagat ccgtccgttc tctgaaaagc
      781 agattgcgct attgaagacc tttgctgatc aagcggttat cgcaattgag aacgtccgcc
      841 tgtttaagga gctcggcgaa cgcaacgcgg acttgcgcga ggcgctggag catcagacgg
      901 cgactgccga agtgctcggc atcatcagcc gctcgccgac ggacatccag ccggtgctcg
      961 atgccatcgt cgagagcgcg gcgcgggttt gtggaataga taacctgggg ctacgactcc
     1021 gtgaggggaa caatctggtt tcgcgggctc aattcggtcc cgttccgccc gggcgcgtcg
     1081 aggtaagtgt tgatgagcca gcaccgcgct gggtacgaga gcacgggacg cttcacgtcc
     1141 ccgacgtcag caagcagaac gatgtccaga tcacaaattc cagtggctcg cgcagttatt
     1201 tattcgttcc ccttcgccaa aaaggcgaat ttattggagc actgagtgca cgccgcaaag
     1261 aggtgcgccc cttcacgccg gcgcagatca aactgctcga aacgttcgca gaccaggcgg
     1321 tcattgcaat tgagaacgtg cgtcttttcc aagaactcaa ggagtcactc gagcagcaga
     1381 ctgcgacgag cgagattctt ggcgtaatcg ccagctcgcc gacggatgtg cagccggtgc
     1441 tggacaccat cgcggaaagc gccgcccggc tgtgcagtgc ggacgattcg tcgattcgaa
     1501 ttgtggaagg cgacattctg cgtctgatga cgcataaagg atcgatcccg ttttttatga
     1561 cgcgggaact gccgattacc cgcggttcgg tagcgggccg ggcggtcgtg gacgacgaac
     1621 tgatccacat tgaggacttg acgctcaggg gaaccgagtt ccctgatgtg caggttgcct
     1681 tggaccggga gggtgttcga accatactgg tcgctccgtt ggagagagaa gggcgggcga
     1741 tcggcgttat tctgatccgg cgcaccgaag tccgcccatt ttctgagaag caaatcgctc
     1801 tgctgaagac cttcgcggat caggccgtca tcgccatcga aaacgtgcgg ttgtttaagg
     1861 aactgcaagc acgaaatcga gatctaactg aggcactgga gcagcagacg gcgaccagcg
     1921 aagttttgaa agtcataagc cgctccactt ttgatcttga gcctgtactg cagactttgc
     1981 tcgagaacgc gactcgcttg tgcggcgccg ctcacggttc catgcatcgg gtcgagggtg
     2041 atgtcattcc ggtcatggcg acatatgggc aatcacagga gctgcaagaa tttctcaaag
     2101 aacatcctgc gcggatcgga aaaaaatccg tcgttggtag agtggtaatg cagcgccaag
     2161 ccgtccacat tcctgatctc agggccgatc cagagtacga ctatgctgct aaattctttg
     2221 aaccgagatc cgtcctggga gtgcccctgc ttagagaagg gctgccgatc ggagtgatta
     2281 tcatctttcg gaccgaagtc gatccgttta ctgaaaagca aatcgatcta gtcacaactt
     2341 tcgcggatca agcggtgatc gccattgaga acgtccgtct acttcaggaa ctccaatcgc
     2401 ggaaccgtga actgactgaa gctctggagc agcagaccgc gaccagcgat gttttgaatg
     2461 taatcgcgca ttcgccgatc gcgctcgaac gggtttacga agcgattgtc gccaacacga
     2521 cgcgcttgtg tgaggccaat atcgcctctc ttttcttgta tgacggcgaa gtccttagta
     2581 cggcggcgag ccacgggacg acgcaggagt ttgccgagca tctcgaacac agccgaccac
     2641 gcccaagccg cgagaccacg actcggctgg ctgctttaga gcacagaaca gttcatgtca
     2701 gtgatctgtt gtccgatcga gccttttctc cgcaaccgcg tcagctttac gaaaaggaaa
     2761 atgtccgcac ggtcttgtcc gtgccgatgc ttcgcgagga taaactcatc ggggtgatca
     2821 cgacgtggcg ccgggaagtg cgcccgttca gcgataaaca gattgcgctc gtcaaaacct
     2881 ttgccgacca ggctgtgatc gccgtcgaga acgtgcgcct tttccaagag atccaggagc
     2941 gcacccgtga gcttcaactt tctctggagg aggtgggcgc tctcagtgaa gtaagccggg
     3001 cggtcagctc gtctctgaac cttcacgagg tgttgagtag ggttgccggc catgcggtaa
     3061 atctctccaa atcggatggc tgcgggatct ttgagttcaa tccggtacgc catgcgtttg
     3121 acgtggtggc gagccacaac ttgagccgag agtttctgga gtctgttcaa acaacgacga
     3181 tcgatcttgg gaacacaacc attggtcaag ccgccgaccg cgcgcagccg atacaagttc
     3241 ccgacatgac gaatgcatat ggccatccgt ttcgggattt cactctgaag gcgggctttc
     3301 tctccgtgct cacggttccc atgagaggca acggagtaac ccgcggtatt gtcctgctgc
     3361 gccgctcgcc gggctcgttc gatgatcgcg tagttaacct ccttacggcg ctggccagcc
     3421 agtctaaagt ggccatcgaa aatgcgcggt tattcagcga gatcgaggac aagggccggc
     3481 agatcgaagc cgcaaatcgc cacaagtcgg agtttctcgc caatatgtcg cacgagctgc
     3541 gcacgccgct caacgcgatc atcggcttct cggaagtttt gctggatccc gcgctccggg
     3601 ttagcgagga agaacggatg cagttcctga cggatgtctt gggcagcggg aagcatctcc
     3661 tcggactgat taatgaagtt ctcgacctcg cgaagatcga agccggaaag atggaattgc
     3721 agatcgacag ggcactgatg caggatgtcg tggaagctgt tgccaatacg atgcggtccc
     3781 tggcaacgaa gaaagggatc cagctccgca ttcacagtga cgagcgcttg gctccgttct
     3841 cgatggatgg ggcacgtgtt aagcaggtgc tgttgaacct cgtcggtaat gcaatcaagt
     3901 tcactcccga agggggaaag gtctggatcc ggacaggtgg gaagaacggc ttcgtgtgcg
     3961 tagaggtggg tgataacggg ccagggatcg cagcggaaga tcaagaaaga atcttcctgg
     4021 aatttcaaca ggccggcagc gacgctggaa aaccgcaggg caccggccta gggctggcac
     4081 tggcgaagaa gttcgtcgag atgcacggcg gaacaatttg gctggagagc gaggttggaa
     4141 agggcagccg gtttttcttt acgctaccaa ccaattgatt ttgcgatttt cgattttgga
     4201 ttgggaaggc agaaagctta tctcccgcaa aggcgcgaag gcgctaagtt tcggaagatt
     4261 tgttaatggg ttgtttgttt aaatctcttt atacttggcg tctttgcgcc ttcgcgggaa
     4321 aaaaatctta tgaatgaaaa gaaaattttg ctggtcgaag acaaccccgt aaatcggcgg
     4381 ctggccggat ttctgctgcg gtcccagggc tacgaggttg ttgaagcaac caatgcagcc
     4441 gaggcgttgc agattctcgc caaggaccca cccgatttga tcgtgatgga cattcaactc
     4501 cctgagatgg atggcctcga agccaccacc aagcttaaag ccgaaccggc aacggcgaag
     4561 ataccagtga ttgcggttac ctcctacgcc atggcgggag atcgggagag ggcgatcgcc
     4621 gccggttgca gtggatacgt taccaagcct atcgacaaaa ccgtatttct cgcagagatc
     4681 agtcgtcatt tgaaataaga ggaccaggtg tggattccgc tacgctaact ccggctggag
     4741 cgcgagtgct gatcgtcgac gatgatggag cctctcgtcg gctcttgcgc gtgcgactgg
     4801 aggcgatgga atgcacggtt gcagcggtcg cggatggccc tgaagccctc gcagagatta
     4861 ggaaacacga gccggaactg atgctcctgg atctccagat gcctaaaatg gggggtattg
     4921 aggtccttaa ggagttggcc aagcaaggag cgaagtttcc ggtagttgtg atcagcgcac
     4981 acggttccat cgaaagtgcc gtcgacgcga tgaaacaggg agcctatgac ttcattacca
     5041 agccgatcga cgccaaacat tttgagatcg tcgtgcgcaa agccttggaa agggagggct
     5101 taaagcgcgg cttggagcta ctcacggaag aaaccgatag ccgccaccgt cttatcgtcg
     5161 ggcagagcaa aagaatggcg gaagccattg agactgcgcg caaggctgct gacagcaagg
     5221 ccacagtgtt gctcctgggg gaaagtggca ccggcaagga aatatttgca cgcgcgattc
     5281 acaattggag cgatagaaag agtcagccgt ttgtggtgat caactgtgta ggcttgtcga
     5341 aggaactctt ggaaagcgag ctgttcgggc acgagaaggg agcgtttaca ggggccgacc
     5401 aacagaaaaa aggcaagatg gaactcgcaa acggcgggac ggtgttcctc gacgaaatcg
     5461 gagatatgtc ccaggagctt caaaccaagt tattgcgatt tcttcaggag cgcgaattcg
     5521 atcgcgtcgg cggcgtcaga ccgattcacg ttgacgttag aatcatctgt gcgaccaatc
     5581 gcgatttgga gactgaagtc aaagacggcc gcttccgcca agatctttac catcgtatta
     5641 acgtcatccc gatcacactt ccgccgttga gagaaagagt ggaagacatc ccggttttgg
     5701 ctcatcactt tgtggaatat tacgccaaag aggcaaaaaa gaacttcaca gagattgtcg
     5761 ccgatgccct gaagcagctc tgtgcctatg attggcccgg caacgtccgc gagctgggca
     5821 acgccatcga aagggctatc gttctgggcc atggcccgta cgtgacggta caggacttgc
     5881 catcgagagt tgctgcgtca gtagtgcggc cgacaatcga tggtcgatcc taccgtgacg
     5941 ccatggaaga ttaccggaaa gaactaattc tcgtagccct agagcgctgc cgcggtaatc
     6001 gggccgccgc ggcgcgactc cttggactgc acgagaaata tttcctaagg cttcttaagt
     6061 cgctggctat cagcgtctaa ccgtctacca agcaaatctc ggtcaatccg tctttaactg
     6121 cctgcagacg ataaattagt aacctaagca ctacggcgaa gtaatcaatc ggttaccctt
     6181 cgcattccat ccgctcgcca cgatccgaaa atcttcgaag aaaatagctc ggtaatatac
     6241 catcttgcct gaccgaacac ttcatcgttg gattggcaca agcgttgcca tctcagatga
     6301 tcgaaggagg gcaggacaat ggcaataaaa atactagttc cattaagaaa acatgaccgg
     6361 ctcgacgaga ttgtgcccta catcgaggag gtggctgagc cgggcgcgag tatcgtgttt
     6421 ctcatccgtc atccggtgag cggcttcaaa tggctgcagg cgtacgctgc gatatcgcaa
     6481 tgtgggatcg agaccgcgct cgccatcaga agaatggctg agagttattc gacgcgcacg
     6541 cgtatgcaat tagcaggtca gaaagttttc tctgcttgcg aagctttgca cagactgggt
     6601 gtgacagtca ccgtggatat ttatgcgagc agcctcagca aagcgttatc caattatgcg
     6661 agtcatgaga atgtgcaatt tgttgtcatg cagcccggaa ttggacagcg aatcgcgcgc
     6721 tatctgcaag gcgtaatttc gattcaaagc atattccggc atccgttttc gttatcgata
     6781 ttcattcgtc cgagtgcgta agggtctctt attttagagt cggacaaagc gcgaaagggg
     6841 ctttcgatgt tatcaaaaga agaaaacgaa atgctttgcc gaacagctcc gggcacaccg
     6901 atgggcgagc tcttccggcg gttctggttg cctgtcgcgc tctcccgcga gctgccaaag
     6961 cccgactgcg caccggtgcg gctccgcatc ctctatgagg acttaatcgc tttccgcgac
     7021 acgaatggaa aagtggggct cctggcgcgg tactgtccgc accgaggcgc gtcgctgttt
     7081 tttggtcgta acgaaggtgg cgggctgcgc tgcgtctatc acggctggaa attcgatgtg
     7141 accggcgcgt gtgtcgacat gccgaatgag ccggcagagt gcagcttcaa acataagatc
     7201 catcagatag cgtatcccac ctgcgaggcc ggcggcgtga tctgggctta catgggtcct
     7261 caagcgagca agccggagat gccgcgactg gagtggacgc tggtgccaca gagtcacgtt
     7321 tacgcgcaca agcgctttca acattgcaat tatctccaga accttgaggg tgaggtggat
     7381 tccagccacg tctcttttct acaccgggag ttccggccgg aaaagttcaa cgcagccatc
     7441 gccggccaaa cgctgctcgc cacggggaag gattcggctc cgaaatttct agtcgaggac
     7501 acggaatacg gcttggcgat tggcgctcgg cgcaactggg atggcgagca gtactactgg
     7561 cgactcactc agtttctcat gccctcgttc acactgatcc cgagcgaagc gggctcgccg
     7621 atcaatttca ctgctgccgt accagtggat gagacaacca tggttggttt caccgtcacg
     7681 tggcttcctg atcgggctct aaatcaggac gatatccgca ccatcgagac gtggacgggg
     7741 gcttacgcgg aagtcgatcc agatactttc gagccggttg ccaaccggca aaatcattat
     7801 cttctaaatc gtgagaagca gcgcaacgaa aacttcaccg gcatgcgcgg tattcgtgaa
     7861 gaagacatcg ccattcagga agatcagtac ggaccgattg cagaccgcac caaagagcat
     7921 ctcggctcaa gtgacgctgg cattatcgcc ctacgaaggc ggttgctgaa gtcggtgcga
     7981 gatctgcaag ctgggcggga gccgccggag ctccactgcg gcgcgaccta tcgtgtacac
     8041 ccgaccgcga gtctcgcgcc tcgtgcaatt tcgttcgagg aggtgactcg ctccgccata
     8101 ccagcggttt aggtacttct gaagagtgcg ttacgacaag agcaagtagt tccaattgct
     8161 ccttgacggc agcggttcaa ctaaactaaa gataggcgga ttgctagaac ggaatttcag
     8221 aatggccgtt cccggtcgcg tctgtatgca gcatctcaac ccggactggg atttccagcc
     8281 gggttttgtt tttccacaat gaataggaag ccctcaaaga aaaggcctgt gaaacaacgt
     8341 acggcgcctt caggagattc tacctccaca ctcaaaaaga cgatcgccgc gcaggcgcaa
     8401 gaaattcgcg agcgagcgga gcagcaggct gccactagcg cgatcctgcg tacgatcgcg
     8461 cgtgcgccgg cagaccttca agcagtgttc gacggaattg ctgagcacgc tgccaagcta
     8521 tgtgatgcgg ccgatgcgtt agtgtggcgg gtcaacggca ctgctcgata tcttgcggct
     8581 cactttggat cggttcccac ctctggagat attggtaccg aggaatatga gttagctagg
     8641 accggacctc ctggccgggc gatactagac cgtgagacgg ttcacgttca tgatcttcgc
     8701 gcctcgtctg cggattttcc cggcgccaag acgcgaggag tagcttttgg gattaggacc
     8761 acacttgtaa caccgttgct gcgcgacggt atggctgtcg gcgctttgca gattcgccgt
     8821 aaggaagtgc gtccgttcac ggatgctcaa atcaaactgc tggagacttt tgccgatcag
     8881 gcggtaatcg ccatcgagaa cgcccggctg atccatgagc agcaggcgag taatcgtgag
     8941 ttaaccgagg cccttcagca gcagaccgcg accagcgaaa ttctgcgggt gatcgccagc
     9001 tcgccgacgg atatccagcc ggtgttggat gctgtggcgg agagcgccgc caggctgtgt
     9061 gaatcctatg acgcgcaaat ctttcgtctg gagaatgagg tcgtgcaccg ggtggcttca
     9121 tacggtccct tgctgatcgc aatggagcat acgcctttaa accgccaatc tcccgccggc
     9181 cgggcgatgg ttgaccggca agcaattcac gtccatgacc tcgcggcaga aattgattct
     9241 gaatatccgg aaattagggg ctaccaacaa cagattggtc atcgcacaac gcttgcagct
     9301 ccgctcttac gcgaaggcgt gccgatcggg gcgattctca tacgtcggtt ggaagtccgg
     9361 cctttcaccg aaaaacaaat agcccttctc aaaaccttcg ccgaccaggc ggtgatcgcg
     9421 atcgagaatg tgcgcttatt ccaagagctc gggcaacgca acgcggaatt gcgtgaggcg
     9481 ctggagcatc agacggccac ctccgaggtg ctcggcatca tcagccgctc gcccacggac
     9541 gtgcagccgg tgctcgatgc catcgtcgag agcgccgcca ggctgtgtga atcctatgac
     9601 gtgcaaatct ttcgtctgga gaatgaggtc gtgcaccggg tggcttcata cggtcccttg
     9661 ctgatcgcga tggagcgtac gcctttgaat cgccaatctc cggcgggccg ggcaatgatt
     9721 gaccggcgac cggttcatgt ccatgaccta gcagcagaga tcaattctga atatccggag
     9781 atcaagggtt accaagagca gattggccat cgcacaaacc ttgcagctcc cctcttgcgc
     9841 gaaggcgcgc cgatcggggc gattctcata cgtcggttgg aagtccggcc tttcaccgaa
     9901 aaacaaatag cccttctcaa aaccttcgcc gaccaggcgg tgatcgcgat cgagaatgtg
     9961 cgcttattcc aagagctcgg gcaacgca
//
LOCUS       DASUVS010000417         7194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_622444, whole genome shotgun sequence.
ACCESSION   DASUVS010000417 DASUVS010000000
VERSION     DASUVS010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622444"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(454..1806)
                     /locus_tag="VF182_22150"
     CDS             complement(454..1806)
                     /locus_tag="VF182_22150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6439836.1"
                     /translation="MANRILLVDDEPFNLDLLEQELADQGHSIATCASGEEALKKLPQ
                     FQPDLILLDYMMPGMNGLEVLKEIRRKGLDTPVIIITAHGTIERAVETMKEGADDFLT
                     KPFDPDHLAFVVKKALERTRLREELEILSEQLGSRFKMVQGKSPSMARAVSEAQKAAV
                     SKSTVLLLGESGTGKEIFARAIHNWSQRKSAPFIPINCVGLSKDLLESELFGHEKGAF
                     TGAHQLKKGRVELADGGTIFLDEVGDISPELQAKLLRFLQEREFERVGGTHQISVDVR
                     IVAATNRNIDKAVSDGTFREDLYHRLNVIPITLTPLRERREDISELATHFLQRFSVET
                     KKQFSTISDDAEMTLMAYHWPGNVRELANVIERAVVLGDGPALTIDMLPARIATLKST
                     DLTDRAEATTYHETINHHRRELILKALSQAGGNRAAAAKALGLHRTHLLKLIKALQLG
                     "
     gene            complement(1906..2994)
                     /locus_tag="VF182_22155"
     CDS             complement(1906..2994)
                     /locus_tag="VF182_22155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6439837.1"
                     /translation="MANKILIVDDEPFNLDLLEQELQEQGYEIERATDGAEALEKLPV
                     FAPDVVLLDYMMPRMNGLEVLKRLRADEKWRSLPVILLTAKAAQEDKITGLDAGADDY
                     VIKPFDAFELLARVRSMLRIKELHDSLEELNRTLSQKVEQQVGQIERMKRLKRYLSPQ
                     IADTLIESNDESLFKTHRREITVVFVDLRGFTAFSDSAEPEEVMELLRTYHAAMGKLV
                     FKYQGTLERFAGDGIMIFFNDPIPCEDHTERAIRMAIEMRSGARELRSGWLKKGYDLD
                     LGIGLAASYATLGNIGFEGRMDYGAVGNVTNLAARLCDEAKGGQILTNQRTLSKVEEL
                     VEFEACEELHLKGFSRAVAAFNITNLKS"
     gene            complement(3063..3431)
                     /locus_tag="VF182_22160"
     CDS             complement(3063..3431)
                     /locus_tag="VF182_22160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6439838.1"
                     /translation="MGKKKKILIVDDTEFNRDLVVQLLEDDFELVIAEDGAEGLKKTQ
                     SEKPDLILMDLGMPVMDGWEATRKIKANDELKHIPIIAVTSHAMAGDEVEARKAGCDD
                     YLPKPIDEDELLKKIKKFLD"
     gene            complement(3516..5969)
                     /locus_tag="VF182_22165"
     CDS             complement(3516..5969)
                     /locus_tag="VF182_22165"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6439839.1"
                     /translation="MTSETIQNPKSKIQNLRYPAFFRLPGGRRRERLLRDYFLISVLL
                     IGGGLITSGLVEIYYRYQESQAQLALLQKEIAGGAAFKIEQFVQEIHNILKGATKSRD
                     IAPKGLTPEFRFELEKLLIIAPAVTEAVALDENGTIQVQASRLRTVLPDAKKDLSTSP
                     SFRQARQGKSYFGQVYFVRGSEPYMTIAVPIERFAGDVIGVLQAEVNLKYIGDVVSGI
                     RVGKAGHAYVISRSGELIAHPDISLVLQRRNVLHLEQARAAFRAPTPGVAASNAIVAT
                     DLQGKDVLSSFALIPTLDWAVVLERPVEEAYEALYASMIRTSTLFMIGLGMALIASLF
                     LARRVVRPVRILRQGVERIGSGDLAYRLNLKTGDEMEILADEFNKMTSQLQESYVNLE
                     QKVEARTKELTESLEQQTATSQVLGVISSSPRDLEPVFETMLTNATRLGSSDYGAIYR
                     FDGKVFSLVTAQYVSPHMTVLANRTFPPDSTTAVGRLGLEKKIVHIPDVTADPKWNPP
                     EEYRKEGIRSVLAVPMLKENNLVGAIVVYRRETQPFTESQIALLKTFADQAVIAIENV
                     RLFKELEEKNEQLEAANRHKSQFLANVSHELRTPLNSIIGFTRLVLRKIEGQIDKLQK
                     ENLHKVLISAEHLLKLINGLLDLAKIEAGRMEVYAETFKIDDVVRVATATVEPMLRNG
                     NVRLVTEMPPELPAIKTDRDKLKQVILNLLSNSAKFTEAGEIKVAAWSENGSLKLAIS
                     DTGIGMNKDALHYIFDEFRQVDMSTTRKYGGTGLGLAIVKRLIDLLGGEIGVESEVGK
                     GSKFTITLPLNPEPLKQFP"
     gene            complement(6311..>7194)
                     /locus_tag="VF182_22170"
     CDS             complement(6311..>7194)
                     /locus_tag="VF182_22170"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439840.1"
                     /translation="AILSPATRPRPVVDALRQRLRELGYTENQNVVFEDRYAEFKVER
                     LPELAAELVKLKPDVIFTHSMTGARAAKQATSTIPIVIGAAGDLVTDGIVASLARPGG
                     NVTGVTLLSSELEGKRLELLKEASRKLSHVAVLVNSANSAWENYPQVLEPVGQALKLR
                     LARAEVRQSTDLEGAFAAMRRNGVDGVLVVSDPMFQTYQSRIAEFAARQQLPSISEVP
                     GFAEAGGLIQYGLSIPEMGRQAAAYVDKILNGANPADLPVERPTKFEFLINLKSAKQL
                     GLTLPPAMLARADKVIK"
BASE COUNT         1574 a         1941 c         1854 g         1825 t
ORIGIN      
        1 aggggagtaa agcgtgactc aggcgaagag aagtagaaca gaaaccctcc catattttgt
       61 agctgaccgg attataaagg cgctataagt tgtatgtcaa gaaaaaaata ccattttaga
      121 aaacattttt tgaatttttt atttctccgg caacgaaaat ccagatctga atatagattg
      181 ccgctgctgg attatttctt gtgaaaaaca aagagagcgt tttattcagc ggtccgcacg
      241 tgagatgccg gtttcggagg cacaaacttt ggcacacttt cttactggac agccaatcgt
      301 gctagcactc acgtccacgg ctggtaatca tcaaggcaat cacgcatgag cgaattaacc
      361 gaacgtgagt ttacccgagg ttcgcaagct ggcgatggtg tccagagcca ttaaactctt
      421 ggtgaaagct tgaacaatca ggaatgaaaa aatctaaccg agctgcaaag ctttgatcaa
      481 ctttagtaaa tgagttctgt gcaaaccaag agccttggca gcggctgcgc gattgccgcc
      541 ggcctgagac agcgctttga gaattagttc gcggcgatgg tgattgattg tttcgtgata
      601 ggttgtcgcc tcagctcggt ccgttagatc cgttgatttt agagtggcaa tgcgcgccgg
      661 cagcatgtct atagtcaaag ccggaccgtc gccgaggacc actgcgcgtt cgattacatt
      721 cgccagttca cgaacatttc cgggccaatg ataggccatc agggtcattt ccgcatcgtc
      781 gctgatggtc gaaaattgct ttttggtttc gacggaaaat cgctgtaaga aatgagtcgc
      841 cagctcagaa atgtcttcgc ggcgttcgcg aaggggtgtg agggtgatgg gaatgacgtt
      901 caagcgatgg taaaggtctt cacgaaaggt gccatcgctg acggctttgt cgatgttgcg
      961 gttggttgcg gcaacgattc taacgtcaac gctgatctga tgtgttccgc caacgcgctc
     1021 gaactcgcgc tcctgcagaa atcttagaag cttggcctga agctccggcg agatgtcgcc
     1081 aacctcatcg agaaagattg ttccaccgtc ggctagctcg actcgtccct tctttaactg
     1141 gtgcgcgccg gtaaatgcgc ctttctcgtg gccgaataac tcgctctcca gaagatcttt
     1201 tgaaagcccg acacaattga tcgggataaa cggggctgat tttctttgac tccagttatg
     1261 gatcgctctg gcaaaaatct ccttgccggt gccgctctcg cctaacagaa gaaccgtgga
     1321 tttgctcacc gcggcttttt gggcttcact cacggcgcgc gccatcgagg ggcttttgcc
     1381 ctgaaccatc ttaaagcgag agcccagctg ttcggaaaga atctctagtt cttccctaag
     1441 ccgggtccgc tccagcgctt tcttgactac gaaggcgaga tgatcgggat cgaatggttt
     1501 cgtaagaaaa tcatcggcgc cctccttcat cgtctcgacc gcgcgctcga tggtaccatg
     1561 ggcggtgata atgatcaccg gagtgtcgag gccttttcgc cggatttctt ttagcacttc
     1621 cagaccattc atgccgggca tcatgtagtc gagcaggatc aagtcgggtt gaaattgcgg
     1681 caacttcttc agagcctcct cgccactggc gcaggttgca atcgaatgac cctgatccgc
     1741 caactcttgc tctagcaggt ctaagttaaa cggctcgtca tcgaccagta aaattctatt
     1801 tgccattgca gatcttggac ggctgaattg tagggtcggc ctgtgaagaa cgtcaaggtc
     1861 tcgatggaat gagtcccaag gtaatctgga ttggttttgg atcgttcacg atttgagatt
     1921 cgtgatattg aaagccgcga cggcgcgact aaagcccttg agatgcaact cttcgcaagc
     1981 ttcgaactcg accagctctt cgactttgct caaggttctc tgattcgtca ggatctgccc
     2041 gcctttggcc tcgtcgcaca gccgcgcggc aagattggtg acgttaccca ccgcgccgta
     2101 atccatccga ccctcgaaac cgatgtttcc cagggttgcg tagctggcgg ccaggccgat
     2161 tcccaaatcc agatcgtacc cctttttcag ccatccgctg cggagctctc tggctccgct
     2221 acgcatctcg atcgccatgc gaattgcccg ctcggtatga tcttcacaag gtatcgggtc
     2281 attaaagaaa atcataatgc cgtcgccggc gaagcgctcc agggtgcctt ggtatttaaa
     2341 taccaacttg cccattgctg cgtgataggt gcgcagcaac tccatgactt cttccggttc
     2401 cgcgctatcg gagaacgcgg taaaaccgcg caggtcgacg aacaccactg tgatctcacg
     2461 tcggtgagtt ttaaataagc tctcgtcatt gctctcgatc aaagtatcgg cgatttgagg
     2521 cgagagatat cgcttcaggc gcttcatgcg ctcaatctgg ccgacctgtt gttcgacttt
     2581 ctgagacaat gtcctattta attcttccaa agaatcgtgc agctctttga tgcgcagcat
     2641 ggaacggact ctggccaaca gctcgaacgc atcgaacggt ttgatcacgt aatcgtcagc
     2701 gccggcatcg agccccgtga tcttgtcttc ctgcgccgcc tttgcggtga gaagtattac
     2761 cggaaggctt ctccacttct cgtcggcgcg gagtcgcttc agaacctcca gtccgttcat
     2821 cctcggcatc atataatcga ggagcacgac atcgggtgca aaaacaggca gtttttcgag
     2881 ggcctcagca ccatcggtcg ctcgttcgat ctcgtagcct tgctcctgca actcttgctc
     2941 gagcagatcg agattgaacg gctcgtcatc gacaattaga attttgttgg ccatgagcgt
     3001 atcgggattt atagttttcg attttgatcc tggtttggcc tcggcccggc tcgactcaga
     3061 catcaatcga gaaatttttt aatctttttc aacaattcgt cttcatcaat cggtttgggc
     3121 aggtaatcgt cgcagccggc cttgcgcgcc tcaacttcat cgcccgccat cgcatgggag
     3181 gtcacggcaa taatcggaat atgttttagc tcatcgttag cctttatttt gcgcgtagcc
     3241 tcccagccat ccatgaccgg catgccgaga tccatcaaga tcaggtcggg tttttcggat
     3301 tgggtttttt tgagcccctc cgcgccgtcc tcggcgatca cgagttcaaa gtcgtcttca
     3361 agcagttgca cgactaaatc gcgattgaac tccgtatcgt cgacaataag tattttcttc
     3421 tttttcccca tgggttccct cgctggctga ttctatctag actcctttga atggttccat
     3481 acgagtttcg cgactgcttg ttgtgcgctg ccgatctacg ggaactgctt caagggttct
     3541 gggttgagcg gcagggtgat ggtaaacttc gaacctttgc ctacttcgct ctcgacgccg
     3601 atctcgccgc caagtaaatc aattagtcgc ttgacgatag ccaatccgag tccggtgccg
     3661 ccatattttc tcgtcgtcga catgtccacc tggcgaaact catcaaagat ataatgcaat
     3721 gcatctttgt tcatgccgat tccggtgtcg gaaatggcca gcttcaagga gccgttctcg
     3781 gaccaggccg ctaccttgat ttctcctgcc tcggtgaatt tagccgagtt gctcaataaa
     3841 ttgaggatca cttgtttgag tttatcccgg tctgttttga tggccggaag ctcgggaggc
     3901 atttcagtga ccagacgcac attgccattt ctcagcatcg gctcgaccgt cgccgtggca
     3961 acccggacta cgtcatcgat cttgaacgtt tcggcataca cttccatgcg gcccgcttcg
     4021 attttggcca gatccaaaag accgttgatc aatttgagca aatgttccgc gctgatcagc
     4081 actttatgca gattttcctt ctgcagctta tcgatctggc cttcgatctt tcgtagcacc
     4141 agccgggtaa aaccgatgat cgagttcagc ggcgtgcgca gctcgtggct aacgtttgcc
     4201 agaaactgcg acttatgccg attggcggct tcgagctgtt cattcttttc ttcgagctct
     4261 ttgaatagac gaacattctc gatggcgatg acggcctgat cggcgaaggt ttttagcagg
     4321 gcaatttgac tttctgtaaa gggttgagtt tccctgcggt aaacaacaat cgcgccgacc
     4381 agattgttct ccttgagcat gggcaccgcg agaacgctcc ggattccttc cttgcggtat
     4441 tcttccggag gattccactt aggatcggct gtgacatcag gaatgtgaac gatcttcttt
     4501 tccagcccaa ggcggccgac tgcggtcgtc gagtctggcg gaaacgttcg attagccaat
     4561 accgtcatat gcggtgaaac atattgagcc gtaactagac tgaagacctt tccatcaaat
     4621 ctataaatcg cgccatagtc ggaactgccc aaacgcgttg cattggtgag catggtttca
     4681 aataccggtt ccaggtctct tggcgaactg ctgataacac ccaagacctg gcttgtcgcc
     4741 gtctgttgct ccaaagactc ggtgagctcc ttggtgcgtg cttcgacttt ttgctccaaa
     4801 ttgacataag actcctggag ctgagaggtc atcttgttga actcgtcggc aagtatttcc
     4861 atttcgtcgc cggtcttgag atttagccga tatgccagat cgccgctgcc gattcgttcc
     4921 acgccttgcc gcaagattct caccggacga acgacacggc gcgctaagaa taggctggcg
     4981 atgagagcca tcccgagacc gatcatgaaa agcgtggagg ttcgaatcat cgaagcataa
     5041 agcgcctcgt aggcttcttc gaccggccgc tcgagcacga ccgcccagtc tagagtcggg
     5101 atcaaagcga acgagctgag cacatcttta ccttgcagat cggtggcgac aatcgcgttt
     5161 gacgcggcga caccaggcgt gggtgctcga aaggcggccc tggcctgttc caggtgcaac
     5221 acattgcggc gctgcagcac gaggctgata tccggatggg cgattaactc gcccgatcga
     5281 ctgatcacat aagcgtggcc tgccttgcct acgcgaatgc cggaaacgac atcgccgatg
     5341 tatttcaaat tgacttcagc ctgaagcacg ccgatgacgt cgccggcaaa ccgctcgatc
     5401 ggcaccgcga tggtcatata gggttctgaa ccgcgaacaa aataaacctg accgaagtag
     5461 gatttgcctt gcctagcctg acggaacgat ggcgaggtcg agaggtcttt cttggcgtca
     5521 ggtaaaaccg tgcgcaatcg cgacgcctgc acttgaatgg tgccgttttc gtcgagcgca
     5581 accgcctcgg tgaccgccgg cgcgatgatc aagagtttct ccaattcgaa gcgaaactcc
     5641 ggcgtcagtc ccttgggcgc tatatcgcgg ctcttggtcg cgcctttcaa gatgttatga
     5701 atctcctgaa cgaactgttc gatcttgaag gcggcgcccc cggcgatttc cttctgcaac
     5761 aaggcgagct gtgcctggct ctcttgatag cgataataga tctcgaccaa gccgctggtg
     5821 atcaagccgc cgccgatcag cagcacggag atcaagaaat aatcgcgcag cagccgttcg
     5881 cgccggcgac caccaggaag tcggaagaag gccggatatc ttaagttttg gattttggat
     5941 tttggatttt gaattgtttc agaggtcatt gttgcctcat ccgtagcatt ttattcgaag
     6001 ctaccgtcat cgcagtgatt tcattggcct ctttcattac ctcagccgcc gaattcatcg
     6061 gcttcttctt ccacaatcgc ccactttgca agaacatcgg ctatggattg ctccccggca
     6121 agcggctcgg tagttagctc cgactgacgt tgctgagcga agtaactgtc ttgcaatcac
     6181 cacccgcaga ctgtgtccgc ggtaatgctt ctataaggcg aatagcatct agggcgaatt
     6241 tctttgtccg gtctttgaag agagcttcgt tcatgggttc taatcgaaaa tccaaatcta
     6301 aaatcgaaaa tcatttgatc accttgtccg cccgggccag catagccgga ggcagggtca
     6361 aacccaattg cttagctgat ttcaaattga tgaggaattc aaacttcgtc ggcctctcca
     6421 ccggcaggtc cgccgggttt gccccattga ggattttatc gacgtaggcg gctgcctggc
     6481 gccccatctc gggaatgctg agaccatact ggatcagacc gccggcctcg gcaaatcccg
     6541 gcacctctga aatgcttggt agttgctgcc tggccgcaaa ttccgcgatc cgactctgat
     6601 aagtttgaaa catcggatcg ctgactacca ggacgccgtc gactccgttg cgacgcatcg
     6661 cggcgaatgc gccttcaaga tcggtggatt gcctcacctc agcgcgcgcg agccgcaact
     6721 tcagcgcctg accgacgggt tcgagaactt gcgggtaatt ctcccaggcc gagtttgccg
     6781 agtttactaa caccgcaaca tgagagagct tacgggatgc ttcctttaag agttcgagcc
     6841 ttttaccctc caattcgcta cttagcaaag tcacgcctgt gacgtttccg cctggacgcg
     6901 ccaggctggc gactatgccg tccgttacca gatcacccgc tgctccgata acgattggaa
     6961 tggttgaagt cgcttgcttg gccgcccttg cgccggtcat cgaatgcgta aagatgacat
     7021 cgggcttgag cttgaccaac tcggcagcca attcgggcag acgctcgact ttgaattcgg
     7081 cgtaacgatc ttcgaacaca acgttctgat tttccgtgta gccgagctct cgtaaccttt
     7141 gtcgaagtgc gtccacgacc ggccgtggcc gtgtggcggg tgacagaatc gcga
//
LOCUS       DASUVS010000418         2246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_185266, whole genome shotgun sequence.
ACCESSION   DASUVS010000418 DASUVS010000000
VERSION     DASUVS010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185266"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            376..1050
                     /locus_tag="VF182_22175"
     CDS             376..1050
                     /locus_tag="VF182_22175"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006106647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6439841.1"
                     /translation="MLLALIFDVDGTIVDSVDLHAEAWRVAFERFGKNFPFDEIRRQI
                     GKGSDQLLPVFLSKQELDSFAEDLDEYRSALFKKEYLPRVQGFARVRELFQRLKNDGK
                     QIVLASSAKADELEAYKKIARIDDLIESETSSDDAEKSKPHPDIFQAALARLKDVAPE
                     NAIVIGDTPYDAQAASKANLKTVGLLCGGWTEEELRGAGCVAIYRDPEDLLLNYEMDL
                     LDLSRA"
     gene            complement(1172..1828)
                     /locus_tag="VF182_22180"
     CDS             complement(1172..1828)
                     /locus_tag="VF182_22180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6439842.1"
                     /translation="MKKDRSLPADLSHFGRLAEYNRKRHFGVTPEPQGKLGRPKKKIL
                     EFVVQKHRASRLHYDFRLEHDGVMLSWAVPKGPSTDPANKRFAMQTEDHPIEYNQFEG
                     VIPEGEYGGGTVMIWDRGTWDPEVADVEEALTKGDLKFKLNGKKLRGSWVLVRMRSRQ
                     WLLIKHRDRDASTDDITVAKPRSAVSRRSLAGIARKAGATLRQLAQAQEADKPAAARE
                     "
     gene            complement(1825..>2246)
                     /locus_tag="VF182_22185"
     CDS             complement(1825..>2246)
                     /locus_tag="VF182_22185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX6439843.1"
                     /translation="ENYVCEVHASGALLVFCGDLNIARTERDVHPKERNPGIIGQRPE
                     ERELFEQILGHGLVDVGRALDPDNENLFTWWPPWRRMRERNIGWRLDYVLASHSLASR
                     ASQCVVYREIGTSDHAPVVAVFDGDDATVEALIREPE"
BASE COUNT          501 a          623 c          577 g          545 t
ORIGIN      
        1 ccgagcgcga attatgaatt tcactggtgg catatcaatt gctgaaatca gtcacgtttg
       61 tagtcttgga gctgtcgcaa tatattgcct gcgttcacct gcagatattg gaaactgtgg
      121 ggtgggttca ggaaaatcaa ctactaagga ggaacggtca tgcctggaaa tattttctat
      181 atcatcggcg tcatcgtcgt ggtgctgttt atcttgggct atttaggatt gcgctaatct
      241 gaacacgtcg gcagacgaag caggctaata ttcgctgctt cgtctgtctc aataccgaac
      301 gccatcatcc tttcctgttt cgttcttggc acaagcgaga gacactgtct catcggcagc
      361 acgtaagctg aacaaatgct actagccctg atattcgacg ttgacggcac aatcgtcgat
      421 tccgtcgatc tgcatgccga agcctggaga gttgccttcg aaaggttcgg caagaacttt
      481 ccatttgacg agatccgccg ccaaatcggt aaaggctccg accaactgct gccggttttt
      541 ctctcgaagc aagagctgga tagcttcgcc gaagatctgg acgaataccg cagtgcgtta
      601 tttaagaaag aatatctccc ccgcgtacaa ggcttcgcga gggtgcggga attgttccaa
      661 cgactaaaaa atgacggcaa gcaaatcgtc ctagcctcgt cggccaaagc cgatgaactc
      721 gaggcctata agaaaatagc tcggatcgac gatctgatcg aaagtgaaac ttcctccgat
      781 gatgctgaga aatccaagcc gcacccggat atcttccagg cggctctggc tcgtttgaaa
      841 gatgtcgcgc ccgaaaatgc catcgtcatc ggcgacacac cctatgacgc acaggcggct
      901 tccaaagcaa acctaaaaac tgttggctta ctgtgcggtg gctggacgga ggaagaattg
      961 cgcggcgccg gttgcgtggc gatttaccgg gacccggagg atttgctcct gaattatgag
     1021 atggacctct tggatttaag ccgcgcatag aaaatccgac cgtaaacgtt tcatggatga
     1081 ttttgcaaaa ccctgacacc tacttagggg cgggtttcga acctgctccc taccgtatcg
     1141 ctatttgaac aacgatacga gaattgcagt tttactcccg cgctgcagca ggcttgtctg
     1201 cttcttgtgc ttgcgccagc tgtcgaagag ttgcccctgc tttgcgcgca attcctgcaa
     1261 ggcttcgccg agagaccgcg gagcgcggtt tggcaaccgt tatgtcgtcg gtggaggcgt
     1321 cgcggtctcg atgcttgatc aacagccact gccgcgaccg catgcgcacg agcacccatg
     1381 atccgcgcag ttttttgccg tttagtttga acttcaagtc gccttttgtg agcgcctcct
     1441 cgacatccgc gacttccggg tcccacgtgc cgcgatccca aatcatcacg gttcccccgc
     1501 cgtattcacc ttccggaatt acgccttcaa attggttgta ctcgataggg tgatcttcgg
     1561 tctgcattgc aaaccgcttg tttgccggat cggttgatgg tcccttcggt acggcccatg
     1621 agagcatcac accatcatgc tctaaacgaa aatcataatg cagccggctc gcgcgatgtt
     1681 tttgaaccac gaattctaaa attttctttt tgggccgacc gagcttgccc tggggctccg
     1741 gcgtgacacc gaaatgacgc ttgcgattat attccgccag cctgccgaag tgactcagat
     1801 cagcgggtaa cgatcgatct tttttcattc aggttctcgg atcagcgctt ctacagtagc
     1861 gtcatcgcca tcgaacacgg cgaccaccgg cgcgtgatcg ctggtgccga tttcgcggta
     1921 caccacgcac tgcgaagccc gtgacgctag agaatggcta gccaagacat agtcgagccg
     1981 ccagccgata ttccgttcgc gcatgcggcg ccacggcggc caccaggtga aaagattttc
     2041 gttatccgga tcaagcgctc gtccaacatc caccaggccg tgcccaagaa tttgctcgaa
     2101 taattccctt tcttccggcc tttggccgat gatcccgggg tttcgctcct tcgggtgcac
     2161 atcgcgctcc gttcgcgcaa tgttgagatc gccgcagaag accagcagcg cgccgctggc
     2221 gtgaacttcg caaacataat tttcca
//
LOCUS       DASUVS010000419         5432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1934287, whole genome shotgun sequence.
ACCESSION   DASUVS010000419 DASUVS010000000
VERSION     DASUVS010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934287"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VF182_22190"
     CDS             <1..768
                     /locus_tag="VF182_22190"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6439844.1"
                     /translation="ANTNSLGYRLTGEHGETWCKLYNDCMHELVKQRPDRFAGMATVP
                     LQDATRAANVLEHSIIDMKFRGAFIGTNVNGQYYNIRDFDPFWAKAQELDVLIVMHPE
                     HIAGAERMGAFGLNAVCGNPADSTLSLGYMMYSGLFDRFPNLKICALHGGGFLPYHLG
                     RFDKEFVTGQGVRPAQSAKPPSAYLKNLCFDTLVYDIDTLEYLRSKVGIDRLMVGTDY
                     PYTLGDWRAVEKVQALSCSESDKEAILQGNARRLLKL"
     gene            complement(889..1884)
                     /locus_tag="VF182_22195"
     CDS             complement(889..1884)
                     /locus_tag="VF182_22195"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439845.1"
                     /translation="MKTKLIIFICALLQLTVWAGAQAQTLRKLRIGYPSISSRQAQLW
                     VAKDEGVFRKHGLDVELIFLRGGQVAIQALTGGDPPLVTIGNVIIANLQGHDIVLVGS
                     VENSYDQSVFARPGTMTRMEQLKGKRFGISGFGSATHNAALILFKKFNLEPIKDVAFV
                     PTGTGPERLAAMGTARIDATFFNPSEVPQALKAGFVEIIQMADIGFEVQGSGLATSRS
                     YIRANRDVVKSALKAYIEGIYFVFTNKAAVMKSLARYMRTDDQEVLDYSYEHYLKRTA
                     KKPYPTMKGIQNLIDMSAPQVAQAKTAKPEQFVDLSFLQELEKEGFFEEMGKRYK"
     gene            1990..2946
                     /locus_tag="VF182_22200"
     CDS             1990..2946
                     /locus_tag="VF182_22200"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439846.1"
                     /translation="MRFRLRILSASILFMVATSPVSAQLKKVRFSVSAASIAEVPFRI
                     AHVKGFYRDEGLDVEVIMIRGAVGMQALLGGSVDYTSSSGSTIAAAVRGIPVKLVFIA
                     SAKPQFDLVVQSEVRSIQDLKGKVVGISSRGGAVDLLMQLLLQKNGLVPNKDVTTIVV
                     GAQEDTVLALRTGRIAAGLFTPPRPLVLQREGFTRLAYTGDYMPTYPSGGIGATDEKI
                     RTNPAEVLAFVKGSVRGLQFAREKRSEAMKILSDYFNIKDQAMADEFFQLYLSRLPAD
                     GGADEAWMKGAIEFTQKSLGDAGKNIPTHRVFDFSFVNKAAR"
     gene            2975..4582
                     /locus_tag="VF182_22205"
     CDS             2975..4582
                     /locus_tag="VF182_22205"
                     /inference="COORDINATES: protein motif:HMM:NF004780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX6439847.1"
                     /translation="MTHYPVLIAGGGPVGTAVAIELGWRGIRCLLVEQTDGTVEFPTT
                     NLANTRTCEHLRRWGIADRMRFESGYPTGYPRNYVFVTRMDGYEIARFDHPANGVPES
                     RSPHSPEGRLWISKPYFDPVLQAHAKSLPHVEIRYQTSLESFEQKAGAVLAEVLEKTT
                     GLKKAVSAEYLVGCDGGRSNIRRALEIKYQGVFSQGMNVAVLFRAPLLQCIKFGPAVQ
                     YQIINSKINGAIAAVDGKELWRLNVRNVKQEQLEAFNAPEKLRYALGEDIPFELLAVR
                     PWTGHCVVAEKYQHGRVFLAGDAAHLNWPAGGFGMNTGVGDAVDIGWKLAAVLQGWGG
                     PRLLDSYTAERKPIAMINVNEAAEMRASFDSQTLFSPKMSEDNDEAKQLREKARAAIL
                     RTRAKEFQHDSAGIELGYRYENSPICISDGTPPPPLDHGLYVPSTWPGARAPHVWLRD
                     GRSTLDLFGKGFSLLNLSGIDTASITEAANRAALPLATIEIDEANVQAIYQRNLVLVR
                     PDGHVAWRGDTVPDDAAAIFDQARGAL"
     gene            4596..>5432
                     /locus_tag="VF182_22210"
     CDS             4596..>5432
                     /locus_tag="VF182_22210"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439848.1"
                     /translation="MIRYALLLIILAVSLTSSAAFAQLKKVRLAIPTVDIVTLPLKMA
                     QAKGFYQKEGLDVEIILIAGALGVRAVLGNSVEFTTASGSILAAAARGIPVKLVSVIT
                     AKPTFDLISEPQIQNFQQLKGKQIGISTRGGTFEHITRLILERNGLNPDKDITILALG
                     RQDDLHLALKSGRISAAIYSPPRSLMLYRDGFHKLAFSGDYLPHYPIGGIGVTDDLIK
                     KRRADIHSFVAATLTGIAYYRQNRSEAIEFIAAELKMSDASLAEQMFDWHRTVLADEG
                     RPS"
BASE COUNT         1210 a         1515 c         1467 g         1240 t
ORIGIN      
        1 gctaacacca acagccttgg ctaccggctc accggcgagc atggggaaac ttggtgcaaa
       61 ctttacaacg actgcatgca cgaattggtc aaacaacggc ccgaccgctt tgccgggatg
      121 gccaccgttc cactccagga cgccacccgg gcggccaacg tgctggagca ttccattata
      181 gatatgaaat ttcgcggcgc ctttatcggc accaacgtca atggccaata ctacaacatc
      241 agagacttcg atcccttttg ggccaaagcg caggaactcg acgtcttgat cgtcatgcac
      301 cccgagcata tcgccggagc cgaacgaatg ggcgctttcg ggctcaacgc ggtctgcggc
      361 aatccagccg attcaacgct ctcgcttggt tatatgatgt atagcgggct gttcgatcgt
      421 tttcccaatt taaaaatatg cgcactccac ggcggcggct tcttaccgta ccatctcgga
      481 cgattcgaca aggaattcgt gaccggccaa ggtgttcggc cggcgcaatc agccaagccg
      541 cccagcgctt atttaaaaaa cctttgcttc gatacgttgg tatatgacat cgataccctg
      601 gaatacctga ggtcaaaagt cggcattgat cgtctcatgg tcggcaccga ttacccatat
      661 actctgggcg actggcgcgc ggtagagaag gtgcaagcct tgagttgctc cgaatccgac
      721 aaagaggcga tcttgcaagg caatgcgcgg cggctgctca aactttagta ttggttcaaa
      781 atttaaggtt caacggctcg gaaaatcaac tctgtgttgg actatcagtg agatttcgtc
      841 cggaagcttt taaaccgtgg aaccttgaac gattgaaccg ttgaacggtt atttgtatcg
      901 ctttcccatt tcttcgaaga atccttcttt ttcgagctcc tgaagaaaac tgaggtcgac
      961 gaattgttcc ggcttggcgg ttttggcttg cgctacctgg ggcgccgaca tatcgatgag
     1021 attttgaatc cccttcatcg tcggatacgg cttcttcgcg gttcttttca gataatgctc
     1081 gtacgaataa tcaagcacct cctgatcgtc cgtgcgcata tagcgggcca ggctcttcat
     1141 aaccgcggct ttgttggtga aaacaaagta gatcccttcg atataggcct tgagcgccga
     1201 tttgacgacg tctcgattgg ctctgatata gctgcgcgag gtcgccaacc cgctgccctg
     1261 aacttcaaat ccgatgtcgg ccatctgaat aatttcgaca aatcccgctt tcaaagcttg
     1321 cggcacttcc gatgggttga agaaagtcgc gtcgatacgt gcagtgccca tggcggccag
     1381 gcgctcgggg ccggtgccag tcggcacaaa cgccacatct ttgatcggct cgagattgaa
     1441 ttttttgaaa agaatcagcg cggcattgtg cgtcgccgag ccgaagccgc taatgccgaa
     1501 tctcttgccc ttgagttgtt ccatccgcgt catcgttccc gggcgagcga aaacgctttg
     1561 gtcataagag ttttccactg agccaacgag cacgatgtcg tgaccctgca aattggcgat
     1621 gatgacgttg ccgatggtaa ccaggggcgg atcgccgccg gtgagcgctt gaatggccac
     1681 ctggccgcca cgtaaaaaaa tcagctcgac atcgagtcca tgcttgcgga ataccccttc
     1741 atccttagcg acccagagct gcgcctggcg cgagctgatc gaaggataac cgatgcgaag
     1801 cttacggagt gtttgagcct gagcgccggc ccacacagtt agctgaagta gcgcgcaaat
     1861 aaagatgatg agcttggttt tcatagcaat atctcgtgtc cttacgtcaa cgtctaacct
     1921 gcgtcaagcc gcgatcccga tgttgacagc tctgtttacc atgtgtttac ttgccgggga
     1981 ggtttcacca tgaggttcag actacggatt ttatcggcct ctattctgtt catggtcgct
     2041 acttcaccgg tttcggcgca gctcaaaaaa gttcgcttct ccgtcagtgc ggcctctatc
     2101 gccgaggtgc cgtttcggat cgctcatgta aaaggcttct atcgtgacga agggctggac
     2161 gtcgaagtga tcatgattcg cggcgccgtc ggtatgcagg cgctgctcgg cggctcggtc
     2221 gattatacct cttcttcggg ttcgacgatt gccgccgcgg tgcgcgggat tcctgtcaaa
     2281 ctcgtcttta ttgcatccgc caagcctcaa ttcgatctcg tcgtacaatc ggaggttcga
     2341 agcattcagg atttgaaagg caaagtcgtc ggtatctctt cgcgcggcgg cgctgttgat
     2401 ctattgatgc aattgctgct gcagaaaaac ggtctcgtcc ccaacaaaga cgtcaccacg
     2461 attgtcgtcg gcgcccagga agacaccgtt ctcgctctca ggaccggtcg tatcgccgcg
     2521 ggattattta caccaccccg gccgctggtg ctgcagcgcg agggcttcac ccggctcgct
     2581 tataccggcg attacatgcc gacctatcca agcggcggca tcggcgccac cgacgagaaa
     2641 atcagaacca accccgcgga ggtgcttgcc ttcgtcaaag gaagcgtgcg cgggctgcag
     2701 tttgccaggg aaaaacgcag cgaagcgatg aagatcttga gcgactactt caacatcaaa
     2761 gatcaggcga tggcggacga gttttttcaa ctatacttga gccggcttcc agcagacggc
     2821 ggcgccgatg aagcctggat gaaaggcgcc atcgagttca ctcagaagag tctcggtgat
     2881 gccggcaaaa acatccccac gcatcgggtt ttcgatttta gcttcgtcaa caaagcggcg
     2941 cgctagtgac agagattatg agcgagcgaa gacgatgacc cattatcccg tccttatcgc
     3001 cggtggcggc cctgtcggca cggcagtagc gatcgaactg ggatggcgcg ggatccgctg
     3061 ccttctcgtc gagcaaaccg acggcacggt ggaatttcca acgactaatc tcgccaacac
     3121 ccgcacctgc gagcacttgc gacgctgggg tatcgctgat cgaatgcgtt ttgagtccgg
     3181 ttatcccacc ggctacccgc gcaattatgt gtttgtcacg cgcatggacg gatacgaaat
     3241 tgcccgcttc gatcaccccg ccaacggtgt tccggaatcg cgttcgccgc atagcccaga
     3301 gggaagattg tggatttcga aaccctactt cgatccggtt ctgcaggctc atgcgaagtc
     3361 gctgccgcat gtggagattc gctaccagac ttctttagaa tcgtttgaac aaaaagcggg
     3421 tgccgtctta gccgaagttc ttgagaaaac aaccggactc aagaaagccg tcagcgccga
     3481 atatctagtc ggttgcgacg gcgggcgcag caatatccgg cgcgctctag agataaaata
     3541 ccaaggagtt ttctcacagg gcatgaacgt cgcggtgctg tttcgcgcgc cgctattgca
     3601 atgcatcaag tttggtccgg cggtgcaata tcaaatcatc aattccaaaa tcaacggcgc
     3661 catcgctgct gtggatggca aagagttgtg gcgactaaac gtgcgcaacg tcaaacagga
     3721 acaactggaa gctttcaatg cgccggaaaa actccgctac gcgcttgggg aagatattcc
     3781 gtttgagttg cttgccgtgc gcccgtggac cggtcattgc gtggtcgctg aaaagtatca
     3841 acacggccgg gtttttctgg ccggtgatgc ggctcatctc aactggccgg cgggcggttt
     3901 cggcatgaac accggcgtcg gcgacgccgt cgatatcggc tggaaactgg cggccgtgct
     3961 ccagggttgg ggcggcccgc gtttgctcga cagttacact gcggaacgca agcccatcgc
     4021 catgatcaac gtcaatgaag ccgccgagat gcgcgccagc ttcgacagtc aaacgctctt
     4081 ttcgccgaag atgagcgagg acaacgatga agcaaaacaa cttcgcgaaa aagcgcgggc
     4141 ggcaattcta cggactcgcg ccaaagaatt ccagcacgat tcagccggca tcgaattagg
     4201 ctaccgctac gagaactcgc cgatttgcat ttctgacggc accccgccac cgccgctcga
     4261 tcacggtctt tatgtgccat cgacctggcc tggagcgcgc gccccccatg tctggctgcg
     4321 tgacgggcgc tcgaccctcg atctgttcgg caagggtttc tcgttattaa atctttcagg
     4381 catcgacacc gctagtatca ctgaagcagc caaccgggcc gcattgccgc ttgccactat
     4441 cgaaatcgac gaagccaacg tacaggccat ttaccagcga aatttggttt tggttcgccc
     4501 cgatggccat gttgcatggc gcggtgatac cgtgccggac gatgccgccg cgatcttcga
     4561 ccaggcgcgc ggcgcgcttt agaaagaaaa aaatagtgat cagatatgcg ttgcttttaa
     4621 taattcttgc cgtttcttta acttcgagcg ctgcctttgc acagctcaag aaggttcggc
     4681 tggcgatccc cactgttgat attgtcacct tgcccttgaa aatggcgcag gccaaaggct
     4741 tttatcaaaa ggaaggattg gatgtagaaa ttatcttgat agccggcgcg ttgggtgtcc
     4801 gcgccgtgct gggcaattcg gtggaattca ccactgcctc gggatcgatt ttagcggcgg
     4861 cggcgcgcgg cattccggtg aaactagttt ctgtgattac ggccaaaccg accttcgatc
     4921 tgatctccga gccgcagatc cagaattttc agcaactcaa aggaaaacaa atcggcatct
     4981 ccacccgcgg cggcacgttc gagcatatca cccgtctgat cctcgaacgt aatgggttaa
     5041 atccggacaa ggacatcact attctggcgc tcggcaggca ggatgatctt catctcgccc
     5101 tcaaaagcgg tagaatctcc gcagcgatct atagtccgcc gcgcagcctc atgctttacc
     5161 gcgatggttt ccacaaactt gccttctcgg gcgattactt gccgcactat cccatcggtg
     5221 gcatcggagt caccgatgat ctgatcaaaa aacggcgtgc cgacattcat tctttcgtag
     5281 cggcaacgct taccggtatc gcctactatc gccagaatcg aagtgaagcg attgaattta
     5341 tcgccgccga gctaaagatg agcgatgcat cgttggccga gcagatgttc gactggcatc
     5401 gcaccgtgct tgccgatgag gggcgtccta gc
//
LOCUS       DASUVS010000420        14063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_842487, whole genome shotgun sequence.
ACCESSION   DASUVS010000420 DASUVS010000000
VERSION     DASUVS010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14063
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842487"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VF182_22215"
     CDS             complement(<1..420)
                     /locus_tag="VF182_22215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEX6439849.1"
                     /translation="MSQRGTPSPSAAEWRQRLSGLQGQRYWRSLEELAQSDEFERIVE
                     GEFPGAASLLSSGLNRREFLRIMGASLALAGLGACAERPTEQIVPYVQPPEERTTGET
                     FDYASAFTLAGAATGILVKSYEGRPIKIEGNPQHPASL"
     gene            complement(432..1088)
                     /locus_tag="VF182_22220"
     CDS             complement(432..1088)
                     /locus_tag="VF182_22220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX6439850.1"
                     /translation="MAQIFHPSTNTVSKLTIFGAVFLIGGLLWLLAELNRSSYVTQAS
                     VVRPQPVPFSHKHHVSGLGIDCRYCHTAVERSAFAGIPSTSTCMNCHSQIWTNSPMLE
                     PVRESYRTGEPLRWNRVHNLAEFVYFNHGIHVNKGVGCATCHGAVDQMPLVSQENSLL
                     MEWCLDCHRNPGRYLRPREEVFNMAWIAPANQSELGTELIRKYNIRTEQLTDCSVCHR
                     "
     gene            complement(1099..1602)
                     /locus_tag="VF182_22225"
     CDS             complement(1099..1602)
                     /locus_tag="VF182_22225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439851.1"
                     /translation="MSDIGTAAILQRLETLERQNFRYRLLGIGTALFVLAAFLMGQSK
                     PNVAGVVEAQSFVLRDKSGNLRGTLAVDNKTDASTLVLADQSGKIRAEFKVANDGNAG
                     IAFGDANGKPRLIMGLKDAGSPDMGLADSQGNLRIGIGLDPDELPAIVMYDDKRNIVW
                     RATGRQP"
     gene            complement(1619..2905)
                     /gene="odhB"
                     /locus_tag="VF182_22230"
     CDS             complement(1619..2905)
                     /gene="odhB"
                     /locus_tag="VF182_22230"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX6439852.1"
                     /translation="MTIEVKVPEVGESITEVEIGDWLKAVGESVEQDEDLVVIETDKA
                     TVEILAPQPGRLSKILKSSGETAQVGEVIGHLEQVEDRSAEAAGEDQTSVSESPEAKK
                     PAKGRRSKVTKPEEPVGTRQSDSGHLPEAQPTVDNGIAGSTEEAPEIISAAQSNRAAD
                     VELEARQPVPDLARPPELAPPVERSEPSVTDDTLGERQETRVPMSQLRRRLAERLVQA
                     QQTAALLTTFNEIDMSAVNQLRHMHQDVFKDKYNVKLGFMSFFVKAAVEALKLLPEVN
                     AEIRDGSIVYRNYFDIGVAVGGGRGLVVPVLRNAQIMSFAAIEIAISDFARRARENKL
                     KPDELQGGTFTISNGGVYGSLLSTPIINPPQSAILGMHAIQERPVARDGAIVIRPMMY
                     VALSYDHRIIDGREAVTFLKQIKTSVEEPVRMLLEV"
     gene            complement(2961..5750)
                     /locus_tag="VF182_22235"
     CDS             complement(2961..5750)
                     /locus_tag="VF182_22235"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX6439853.1"
                     /translation="MSSKIQPVSNSANLSYIEAIYAAYLADPTSVDHDWRDYFAALNG
                     DALVDQTAHTQLSASGNGASAHVKGAVSSVKFSVSTLQEQVNEMIRAFRSLGHKAARI
                     DPLGLSNDPQPSLDPEFYGLTADHLKRLFPGAGLPWNGPLRIGEIVEGLRRTYCGAIG
                     VEFTHIDEPAVRNWLQRRMEESHNRLELSHKEKLRILGRLTDAVTFEEFVRKKFVGAK
                     SFSLEGAESLIPLLDFAIDHAARDGLQEIVLAMAHRGRLNVLANIIGKSPWEIFREFD
                     DPLTAESGGGDVKYHLGYSNDYETASGAKIHLSLCFNPSHLEFVNPVALGRMRAKQDR
                     AGDQQHGRGMALLIHGDAAFAGEGVTQETLNLSQLDGYSIGGALHVVINNQIGFTTSP
                     KDARSSRYATDVAKILQVPIFHVNGDDPEAVAQVVRLAMDFRRTFKRDAVIDMYGYRR
                     WGHNEGDEPTFTQPKLYQAIKKHRPVRDLYLEKLLSERIIDAGEANRLMVQRRGDLEK
                     ELERARSATAVPAPQSFGSFWAGYCGGLEQTAAPARTGVPREQLSALLDSLARIPKDF
                     QPHPKIKRGMELRREMAHGTRPIDWSAAEALAFASLAVQGIRVRLSGQDSARGTFSHR
                     HAVLRDFQDGHPYVPLQHLQRGQAPVEMINSPLSETGVLGFEYGYSLDYPEALTLWEA
                     QFGDFVNAAQVIIDQFITSAEEKWHRLSGLVLLLPHGFEGMGPEHSSARIERFLQLCA
                     KDNIQVLYPTTPAQYFHCLRRQVLRKWRKPLVMMTPKSLLRHPSAVSTLDALADGEFE
                     TVIADRGAQQDCQKILLCSGKVYYDLEKKRSQMNRNDVAIVRIEQFYPVPEERLRTIL
                     QSYAAGTPVVWVQEEPRNMGAACFWHLQLGNKLFDRFPFSVVARAASASPATGSASRH
                     HHQQTELLAAAFGSQ"
     gene            6150..7715
                     /locus_tag="VF182_22240"
     CDS             6150..7715
                     /locus_tag="VF182_22240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6439854.1"
                     /translation="MDFQFAFRANIAARVEPARKSGSEDPLDEEKLELDATEPPSTEL
                     NNVSDDAAEETVETQAAADNEPLTIYLKEIRSVQLLTRRQEIDIAKQREAGELQVLES
                     ILSTPIALQHVLGLGAKIQNDEIRLSDVIGGLTREDDVQGMPAEVECDRRDAFLKSVS
                     SLERRWRNLESLQRKAREKKTSERARLLLRRSIVKKKQGMIGILRDLGLTRLQLTIIA
                     ESLQQSKMRIVACERMLAHSGGQQEDYLRELREIEQTIGMNSEELKWHVDAISQGEQQ
                     AAAAKKLFIESNLRLVVSIAKRFRRFNADLADLIQEGNIGLMRAAEKFDHRLGYRFST
                     YATWWIRQFIVRSMMDTGHMIRIPAHMVEARSKLLRAFECLAQKLGKTPSAADLAMDT
                     GLNPQEMQKIFTLPGEHLSLNAPLGDDPEETLQHYARDRCTEDPSEQVIKKDLCSVTR
                     KSLSVLNSRQEMVLRHRFGIDLTREHTLQEIGDMFLITRERVRQIEAKALRQLRLAAV
                     KRKTKKAAGLLSA"
     gene            7865..8170
                     /locus_tag="VF182_22245"
     CDS             7865..8170
                     /locus_tag="VF182_22245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439855.1"
                     /translation="MYVSRLTFLTVPGKTGELEDELKTLCDIVKRAGGENTRILRTHF
                     ASPDAPNVVFKQDAPELKTLEEQIRKVTAEPSFQDWSKKVSPLLRQSPNREIYKVVD"
     gene            complement(8252..9295)
                     /locus_tag="VF182_22250"
     CDS             complement(8252..9295)
                     /locus_tag="VF182_22250"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX6439856.1"
                     /translation="MRQTNVWSIVLAGGEGTRVRGFLKQLCGGTGLKQFSPIIGRRSM
                     LQHTLDRMQRLVPPERTLIVVGEHQRVEASRQLAHWPQQNIIYQPSNRDTAPGILLPF
                     SFITHQDPSATVVVSPSDHFVRDENRFMDSLATAIGDLKSHHDKMILLGMTPEQGQET
                     EYGYIQVKKHPRATRTRPVAAFVEKPPLPLARRLINEGALWNTMVFTVRSTTLWQMVR
                     DTVPSLYNAFGLIQSMLGSIHAPGFIRHIYQTIPNVNFSSEICAPLAKKLRVLQVPNV
                     GWSDWGTVASILRSLIEMGQLETFRLRLEESQVHPSWYAASLAEIDPQKDLPSEAVYA
                     SLRTEAEAYLSPD"
     gene            10134..10616
                     /locus_tag="VF182_22255"
     CDS             10134..10616
                     /locus_tag="VF182_22255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439857.1"
                     /translation="MKQAFGSGDRGEFSGSTRFESKDADFSDDILTQISSMPLGIHAA
                     SNQNRRMEVMGEFILVQAILREAIRTYQKYAVKKGTRASRLFRDVNEWFSSDDRQWFF
                     SFVNVCDTLGLEPTYIRTGLKLWRERKLQATGAAPTISEYQPIPKTLRRRLVANELRS
                     "
     gene            10698..11060
                     /locus_tag="VF182_22260"
     CDS             10698..11060
                     /locus_tag="VF182_22260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439858.1"
                     /translation="MLNAEQEIERLVDEINRAIQRSPADQRHELRSYASSLINDITAE
                     SSAQSSPSSAQTERRPFGLVAAGLALLVIGAGFALIIPLIGLTLAALGVVLVIWGGVV
                     GWWMTRTESAPAGGHGRR"
     gene            11312..12664
                     /locus_tag="VF182_22265"
     CDS             11312..12664
                     /locus_tag="VF182_22265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6439859.1"
                     /translation="MSATILVCDDDSVPRRNIASVLEEEGYTVHQAEDGKAAIDAVNQ
                     IDFDLVLTDLKMPGVDGLSVLKHVRSISPQTLVILMTAFSSVDTAVEAFRLGAQDYIL
                     KPIVIEDVIRKVGRFLEHKNLVWEVEMLRREINRSSEPTSLVGSSKPIQDIFKIVSKV
                     AATNSTVLITGESGVGKEVVARTLHLQSLSKDKVFLPVNCSAIPDTLLESQLFGHAKG
                     AFTGAINAQEGLFQKAQGGTIFLDEIGEMPLTLQPKLLRAIETKEVLAVGSTQPVRLN
                     VRIIASTNRDLEREANEGKFREDLFYRLNVVHVWVPPLRERREDIPLLVDYLISRHNR
                     EMKKAYKGADNSAMKVLMSLPWKGNIRELDNVLERAMILGDGEWITPEHLPESEIPEN
                     DRNVTANNLRVAVESYEKSHIANVLRDTAGDRTRAAELLGLSRSSLYRKMEKLNIRDD
                     "
     gene            12877..>14063
                     /locus_tag="VF182_22270"
     CDS             12877..>14063
                     /locus_tag="VF182_22270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEX6439860.1"
                     /translation="MTRNRPATRRQKKQNAGTAVRTPNRSAESALSSSTEPRIRAIIE
                     GVKPEIDCGRFPVKRVVGDRVTVEADIFTDGHDALSARLLFRAQGDADWNEARMEPLV
                     NDRWRGVFDITALTDYFYTLEAWVDRFKSWRDGLSKKAAAKQDVGLELIEGAELVALA
                     AQRAQGGDAETLTAYAAALRAKEPGAVQKALEEELAALMSKHAERRWAARYDKELRIG
                     VDRPMAEFSSWYEIFPRSCADQPRKHGTLRDCMARLPGIAALGFNVLYLPPIHPIGWA
                     YRKGKNGTLSPEPDDVGSPWAIGSEEGGHKSVHPLLGTLADFKQLVEKAREHEIEIAL
                     DIAYQCSPDHPYVKEYPQWFRKRPDGTIQYAENPPKKYQDIYPIDFETEDWDALWDEL
                     KRVL"
BASE COUNT         3172 a         3839 c         3904 g         3148 t
ORIGIN      
        1 cagactcgcc ggatgctgcg ggttgccttc gattttgatc ggcctgcctt catagctctt
       61 gaccagaatg ccggtggcag ctcctgccag tgtaaaggcg ctggcataat cgaaggtttc
      121 cccggtggtg cgctcctcgg gaggctggac atagggcacg atttgctcgg tgggcctctc
      181 ggcgcacgcg ccgagaccgg ccagcgccag tgaagcgccc atgatccgta aaaactcgcg
      241 ccggtttagt ccgctgctca acagtgaagc ggcgccggga aattctcctt cgacaatccg
      301 ctcgaattca tcgctttgcg ccagctcttc caagctgcgc cagtaacgtt gtccttgcag
      361 tccagagagg cgctggcgcc attctgcggc gctcgggctc ggggttccgc gttgactcac
      421 cttgttgtcc ctcatctgtg gcaaaccgag cagtcggtaa gctgttcggt ccgaatgttg
      481 tattttcgaa ttagctccgt gccgagctcc gactgattcg ccggtgcaat ccaggccata
      541 ttgaaaactt cctcgcgggg acgaaggtag cgcccgggat tgcgatgaca atccagacac
      601 cattccatca gcagggaatt ttcttgagaa accagcggca tctgatcgac ggcgccatga
      661 caagtcgcac agccaacgcc tttgttcaca tggatgccat gattgaaata gacaaactcc
      721 gccaggttgt gaacccggtt ccagcgcagg ggttcacccg tgcggtagct ttcgcgcacc
      781 ggttcgagca tcgggctgtt ggtccaaatc tgcgaatgac aattcatgca ggtggacgtc
      841 gaaggaatgc cggcaaaggc gcttcgctcc accgctgtgt ggcaataccg gcaatcgata
      901 cccaggccgc tgacatgatg cttgtggcta aacggcaccg gttgcgggcg caccacgcta
      961 gcctgagtga cataagatga acggttcagc tcggctaata accacagaag gccgccgatc
     1021 aaaaacacgg cgccaaaaat agttagcttg gagaccgtgt tggtgctggg atgaaaaatt
     1081 tgagccattc accgccgctt agggttgccg cccggttgcg cgccagacga tgttgcgctt
     1141 gtcgtcatac atgacgatgg cgggaagctc gtccggatca aggccgatgc cgatgcgcag
     1201 attaccttgg ctgtcggcta agcccatgtc cggcgaaccg gcgtctttca aacccataat
     1261 cagccgcggc tttccattgg cgtcaccaaa ggcaatcccg gcattgccgt cgttggccac
     1321 tttgaattca gcacggatct tgccgctctg atccgccaaa accagagtcg atgcgtcagt
     1381 cttgttatcc accgcaagag tcccgcgtaa attgccgctc ttgtctcgca acacgaaact
     1441 ttgtgcctcg acgaccccgg cgacgttggg cttgctttga cccatgagga aggcggccaa
     1501 cacgaacaga gctgtgccga tgcccaaaag gcgataacga aaattttgcc gctcgagagt
     1561 ttcgaggcgc tggagtattg ctgcagttcc tatatcactc attctgatcc ccgctcgatc
     1621 aaacttccag taacattcgc acgggctctt cgacggatgt tttgatttgt ttaagaaatg
     1681 tgaccgcttc gcggccatcg atgatgcgat gatcataact caaagcgaca tacatcatcg
     1741 gccgaattac gatcgccccg tcgcgcgcca ccggccgctc ttggatggcg tgcatcccca
     1801 gaatcgcgct ctgcggtgga ttgatgatcg gcgtcgacag caacgagcca taaacaccgc
     1861 cgttgctaat ggtaaaagtt ccgccttgca actcgtccgg cttcaatttg ttctcccggg
     1921 cgcgccgggc aaagtcgctg atggctattt cgattgccgc aaagctcata atctgagcgt
     1981 tgcgcagcac cggcaccacc aacccgcgtc cgccgcccac cgctacgcca atgtcgaaat
     2041 agttccggta tacgatactg ccgtcgcgta tctctgcgtt gacttccgga agaagcttga
     2101 gcgcttcgac ggcagctttg acgaagaaag acatgaagcc gagcttgacg ttgtatttgt
     2161 ccttgaagac atcttgatgc atgtgccgta gctgattgac tgcggacatg tcgatctcgt
     2221 taaaagtcgt caacagagcg gcggtttgct gcgcttggac tagtcgttcg gccaggcggc
     2281 ggcgcaactg gctcatcggc acgcgtgttt cttggcgttc gcctaacgta tcgtcggtca
     2341 ccgagggttc tgacctctcg acgggcggcg caagctccgg tggtcgcgcc agatcgggga
     2401 ccggttgtct cgcctcgagc tcgacatcag cggctcggtt ggattgcgcc gcggaaatta
     2461 tttccggcgc ttcctctgta gatcctgcaa tgccattatc gacggtaggc tgagcctccg
     2521 gcaaatggcc ggaatcagat tggcgggtac cgaccggctc ttccggcttg gttactttac
     2581 tgcggcgccc ttttgccggt tttttagctt cgggtgattc actgacgctg gtctgatctt
     2641 ctccagcggc ctcagcactc ctatcctcaa cctgttccag atgtccgatc acttcgccca
     2701 cctgcgccgt ttcaccgctg ctctttaaga ttttgctaag cctgcctggt tgaggcgcaa
     2761 gaatttcgac ggtcgcctta tcggtttcga taaccaccag atcttcgtcc tgctcaactg
     2821 attcgccgac cgctttgagc cagtcaccga tttcaacctc ggtgatcgat tcgccgacct
     2881 cgggaacctt aacctcaatg gtcattgccg accttgaatt gacgggtcat agacagcatg
     2941 gcttctagcg ccgcgcccgg ttactggctt ccgaacgcgg cagccaataa ctcggtttgt
     3001 tgatgatgat gccggcttgc cgagccagtt gccggactgg cggaagcggc gcgcgcgacc
     3061 accgaaaagg gaaagcgatc gaaaagcttg ttaccgagct gtaggtgcca aaagcatgcg
     3121 gcgcccatgt tgcgcggttc ttcttgaacc cacactaccg gcgttccggc tgcatagctt
     3181 tgtaagatcg tgcgcagccg ctcttccggc accggataga attgctcgat gcgaacgata
     3241 gcgacgtcat tccggttcat ctggctccgc ttcttctcca ggtcataata tactttgccg
     3301 ctgcataaaa ggattttctg acaatcttgt tgcgcgccgc gatcggcgat aaccgtttca
     3361 aattctccat ccgccaatgc gtccagagta gaaactgcgc tgggatgacg cagcaagctt
     3421 ttgggagtca tcatgaccag cggcttacgc catttgcgca gcacttgtcg acgcaggcaa
     3481 tgaaagtatt gcgccggggt tgtcggataa agcacctgga tattatcctt ggcgcaaagc
     3541 tgcagaaacc gttcgatgcg cgcgctcgag tgctccggtc ccatgccttc aaagccatgc
     3601 gggagcaaca acaccaggcc gctgagccgg tgccattttt cttccgcact ggtgatgaac
     3661 tgatcaatga tgacttgtgc agcattgacg aaatcgccga actgcgcttc ccacaacgtc
     3721 agagcctccg gataatcgag actgtagccg tattcgaaac ccaaaacgcc ggtttcggaa
     3781 agagggctgt tgatcatctc cactggagct tgcccgcgct gcaagtgctg caggggaaca
     3841 taaggatgtc catcctggaa atcacgcagc acggcatgac gatggctgaa agttccgcgc
     3901 gcactgtctt gcccggatag gcgcacccgg atgccttgaa cggctaaact cgcaaatgcc
     3961 aaagcctctg cggcggacca gtcgatgggt cgtgttccat gggccatctc tcgacgcagc
     4021 tccatccctc gcttgatctt agggtgcggc tggaaatctt tagggatgcg cgccagggag
     4081 tccaataatg ccgagagttg ttcgcgtggg acgccggtgc gggccggcgc cgctgtctgt
     4141 tcgagaccgc cacagtagcc cgcccagaag ctaccgaagc tctgcggggc gggaaccgcg
     4201 gtggcgcttc gcgcccgctc caattccttt tccagatccc cccggcgctg caccatcagt
     4261 cggtttgctt cccccgcatc gatgattctt tccgatagca gtttctccaa atagagatcg
     4321 cgcaccgggc gatgcttctt tatcgcctga tagagtttcg gttgcgtgaa cgtcggttca
     4381 tcgccttcgt tgtgacccca gcgtcgatag ccatacatat cgatcaccgc gtcgcgttta
     4441 aacgtgcgcc ggaaatccat cgccagccgg accacctgcg ccaccgcttc aggatcatcc
     4501 ccattgacgt gaaaaatcgg cacttgcaaa atcttggcga catccgtggc atagcggctc
     4561 gaacgggcat ccttggggga agttgtaaaa ccgatctggt tgttgatcac cacgtgcaga
     4621 gcgccaccaa tgctgtagcc gtccagctgg cttaagttta gggtttcctg cgtcactcct
     4681 tcgccggcga aagcagcgtc gccatgaatc agcagtgcca ttccccggcc gtgttgctga
     4741 tccccggcgc ggtcctgttt ggcgcgcatg cgacccagcg ccaccgggtt gacgaattcg
     4801 agatggctcg gattaaaaca aagcgaaaga tgaattttgg cgcccgaggc ggtttcgtaa
     4861 tcattgctgt aacccaggtg gtatttcacg tcgccgccgc cgctctcggc tgtaagagga
     4921 tcgtcgaatt cacgaaaaat ctcccacggg ctcttaccga tgatattggc aagcacgttc
     4981 aagcggccgc ggtgcgccat cgccaggacg atttcttgca agccgtcgcg cgccgcgtga
     5041 tcgatggcaa aatccagcaa aggaatgagg ctctcggcgc cttcgagcga aaagcttttg
     5101 gcgccgacaa atttctttct gacgaattcc tcgaacgtga ccgcatcggt gaggcgcccc
     5161 aggattctaa gcttttcttt gtggctcagc tcaagccgat tgtggctttc ctccatgcgc
     5221 cgctgcagcc agtttcgaac cgccggttca tcgatgtgcg tgaattcgac gccgatggcg
     5281 ccgcagtacg tacggcgcaa gccctcgaca atctcaccga tacgcaacgg gccgttccaa
     5341 ggcaaccccg cccccggaaa caggcgtttt aaatgatcag cggtaagccc gtaaaactcc
     5401 ggatcgagag atggttgtgg gtcattggac aaaccgagag gatcgatgcg agcagcttta
     5461 tgcccgagac tccgaaaggc tcggatcatc tcgttgactt gttcttggag agtggaaacg
     5521 ctgaatttga cgctgctcac cgcgcctttt acatgcgcac tggcgccatt gccgcttgcc
     5581 gatagctgcg tgtgcgcggt ttggtcgact aaagcatcgc cgttgagcgc ggcgaagtag
     5641 tcgcgccaat cgtgatctac cgaagttggg tctgcaaggt atgcggcgta aatcgcttcg
     5701 atatatgaga gattagctga attggaaact ggttggatct tgctgctcat gatcgaatca
     5761 aatcagaaaa aaaaatccaa tcaacaggca gcattcctgt ggatcaactt cggcatcctt
     5821 gcgcaaagtt cttccatagc cctcgcatca cgagaaagct ggtgatggct tcatcgaacg
     5881 atgaaacgcg ggcgccacct aacgatggtg tgttaatgat caaagttcga gcaatgccta
     5941 tgccacgaac gcccggtggg gcatcgaatg aacaaccata aggtttgcct cgcacgtcct
     6001 gccgccgaga caaccggagt ggctgatgtc cgatctgcgc gacaggaggc tcgtggagcg
     6061 gagttctacc ttctacaact agtggcatat ttgttgcgca taagtaactt gctgcgccgc
     6121 cgtcgactaa aattctcgag gagttaaaga tggattttca gttcgctttt cgcgcgaaca
     6181 tcgctgctcg ggttgagccc gcgagaaaat ccggttcaga agatccgctc gacgaagaaa
     6241 agcttgagct cgatgcgaca gagccaccat cgaccgagtt gaataacgtg tccgatgatg
     6301 cggcggaaga gacggtagaa acgcaagctg ccgccgacaa cgaaccgcta acgatttact
     6361 tgaaggaaat tcgctctgtt cagctgctca cgcgccggca ggaaattgac atcgccaagc
     6421 aaagggaagc cggtgagttg caagtcctcg aaagcatcct atctacaccg attgctctgc
     6481 agcatgtgct cggcctcggg gccaaaatcc aaaatgacga gatccgcttg tctgacgtta
     6541 tcggcggatt gacgcgagaa gatgacgttc agggtatgcc ggccgaggtc gagtgcgatc
     6601 ggagagatgc atttctgaaa tccgtaagct ccctcgagcg gcgctggaga aatctcgaat
     6661 cactgcagcg caaagcgcgg gaaaaaaaaa cctcggaacg agcgcgcttg ttgctgcggc
     6721 gttctattgt caagaagaag caaggaatga tcggcatact ccgggatctc ggtttaacaa
     6781 ggctgcaact gacgatcatc gccgaatcct tgcagcagtc gaagatgcga atcgtcgcct
     6841 gcgaacggat gctggcgcat agtggaggcc aacaggaaga ttacctgcgc gaactccgtg
     6901 aaattgaaca aacgatcggc atgaattccg aagagctgaa atggcatgtc gacgcgatct
     6961 ctcaaggtga gcaacaagca gccgcggcca agaagctttt catcgaaagt aatcttcgtt
     7021 tggtagtcag cattgccaaa cgtttccggc gtttcaacgc ggatctcgca gatttgattc
     7081 aggaagggaa tatcggtttg atgcgggccg cagagaagtt cgatcatcgt ctggggtatc
     7141 gattttctac ctacgccact tggtggattc ggcagtttat cgttcgcagc atgatggata
     7201 cggggcatat gatcaggatt cccgctcaca tggtcgaagc ccgaagcaag ttgctgcggg
     7261 ctttcgagtg tcttgcgcaa aaactcggca agaccccctc cgcggcggat cttgccatgg
     7321 acaccggcct gaacccacag gagatgcaaa aaatatttac ccttccgggc gaacatctgt
     7381 cgttgaatgc gccgcttggc gatgaccctg aggaaacgct gcagcattac gccagagacc
     7441 gttgcaccga ggatccttcc gagcaagtta taaaaaagga tctttgttcc gtcactcgaa
     7501 aatccttatc ggttctaaat tcgcggcaag aaatggtgct gcggcatcgg ttcggcatcg
     7561 atcttacccg ggaacatacc ttacaggaga tcggcgacat gtttctgatt actcgcgagc
     7621 gcgtgcgtca gatcgaggcc aaggcgttgc gccagctgcg gcttgcggct gtgaagagaa
     7681 agaccaaaaa ggcagccggt ctgctttcag cttagccgat actattttcg aacagagaac
     7741 gggcacttgc acagaagcga ttgctttcta cccagaagcg ctggcagacg gttgacttca
     7801 ttgaccgttg caatgtcaac agcgcgctgt gagcgcgtaa ataaaaagca gggaggatct
     7861 taggatgtat gtatcgcggc taacttttct tactgtaccc ggcaaaaccg gtgaactaga
     7921 ggatgagcta aagactttgt gtgatatcgt caaacgggcc ggcggtgaga atactcgaat
     7981 cctgcgcacc cattttgcct cacccgacgc gcccaatgtg gtgtttaagc aggatgcgcc
     8041 tgaattgaag actttggaag aacagattcg caaagtcaca gccgagccga gcttccagga
     8101 ttggagcaag aaggtttcgc cgctgctacg gcaatcgcct aatcgcgaaa tatataaggt
     8161 ggttgattag cgcgcgccgg gactccccga gcccagaccg gcgcgtttat actgcatcaa
     8221 gtgtaggctc agccatcact ggcggtggaa attaatcggg agataaataa gcctcagctt
     8281 cggtccgtag ggaggcatat actgcttcgg acggaaggtc cttctgaggg tcgatttccg
     8341 ccaatgaagc ggcgtaccag gaagggtgta cttggctctc ctccaagcgc aatcggaagg
     8401 tttcgagctg acccatctcg atgagcgagc gcagaatgct ggcaacggtc ccccaatcgc
     8461 tccagccgac attcggtact tggaggacgc ggagcttttt ggcaagcggt gcgcagatct
     8521 cagatgaaaa gttgacgttc ggaatcgtct ggtaaatgtg ccggataaag ccgggcgcat
     8581 ggatactgcc taacatcgac tgaatcagac caaaagcatt gtacagcgat ggcacagtgt
     8641 cccggaccat ctgccatagc gtagtgctcc tgacggtgaa aaccatcgtg ttccataagg
     8701 cgccttcatt gatcaaacgc cgcgcaagcg gcagcggcgg cttttcgaca aaagccgcca
     8761 ccggccgcgt tcgagttgcc ctcggatgtt tttttacttg gatataacca tattcggtct
     8821 cctgcccctg ctcgggggtc atgcctagca agatcatttt gtcgtgatgc gactttagat
     8881 cgccaatcgc cgtcgccagc gaatccatga aacgattttc gtcgcgcacg aagtgatccg
     8941 aaggcgaaac tacaacggtg gcggacggat cctggtgagt aatgaacgaa aacggcaaca
     9001 agatgcccgg cgcggtatcg cgatttgacg gttgataaat tatgttctgt tgcggccaat
     9061 gggctaactg gcgcgatgcc tctacacgtt gatgctcgcc cactactatg agcgtacgtt
     9121 cgggcggcac tagccgctgc atacgatcaa gggtgtgttg caacatcgag cgccggccga
     9181 tgataggcga aaattgcttg agaccagtac cgccgcagag ctgtttgaga aaaccacgaa
     9241 ctcgcgtacc ttcaccgccg gctaatacga tcgaccatac attagtttgg cgcactgaca
     9301 gactcccggg ctgctgattg ctgatgaata gcgggcattc tcattggcaa cctctgtgcc
     9361 aaatcgtgta acaaagaaat tgggtcgcga acggccgcgt tgatccaact cgttccagtc
     9421 gcgggacggc gggttagacc tgtttcgcgc cagcgacaca caatctacca ttgtcaacat
     9481 ggtattgcgg agggttttcc tacctattta gatatttcgg atcgacaatc gcctggccgc
     9541 tatacaacct catagcgttt gaccgacggc cgatcgccgt gcaaatgccg cgcatgcttt
     9601 ttaatttcct cgtgggcgtc actgacccta tgagcatcta tactcttttg atccttccac
     9661 tctgaatagg agataaattc cccgggatca taactcgagc gaagcagttt ctccgaaagg
     9721 caaccaggct gcttgatcat caatggagcg caactctctt tccagatctg ctctgcgttc
     9781 tttgcttcct cgggcacgac tcggacaaaa atagccgaac gataggaatg atgaattcct
     9841 ccaccggttt ttgaaatctt aatagaacag atgccatgcc agggctcacc ccaagtccct
     9901 gatggtacgc agcctgtggc gcttgatggt gtggtcttta acgatgaaca atcgaggccg
     9961 gcgggtagat gtgatggtag tcaccgtccc accaaattag ctgatgaagg gctgttgtcc
    10021 cacatcgtgg aatgacaaac agcctagccg tgcaattcca tccagttgaa gaagtggcat
    10081 acacgttgct cggtatgaac agcgagatca aactggttgc ggaggatcaa gtaatgaaac
    10141 aggcattcgg aagtggcgat cgaggagaat tttccggcag cactcgcttt gaatccaaag
    10201 acgcggactt ttccgacgat attttaactc agattagcag tatgcctctt ggaattcatg
    10261 cggcaagtaa tcagaaccgt cgcatggagg tgatgggtga gtttatcctc gtgcaagcga
    10321 tacttcgcga agccatccgg acgtatcaaa agtatgccgt aaaaaaaggc actcgcgcat
    10381 cgcgattgtt tcgcgatgtc aatgaatggt tctcatcgga tgaccggcaa tggttcttct
    10441 ccttcgtcaa tgtttgcgat actctcggcc tcgagcctac ctatatacgc accggcctga
    10501 agttatggcg cgagcgtaag ctgcaggcca ccggtgcggc tccgaccatt tcggagtatc
    10561 agccgattcc caagaccttg cgccggcgac tggtcgccaa tgaactgaga agctaacaac
    10621 agatgttcgc cgccgtctag cgtctactcg caacccattg gcttgcaacg gattgctgga
    10681 gggatgtcga tctccaaatg ttaaatgccg aacaggaaat cgagcggttg gtcgacgaga
    10741 tcaaccgcgc tattcagcgc agtccggcgg atcagcggca cgaactccgt tcgtatgcct
    10801 cgtccctaat taacgatata actgcggaat catcggccca gtcgagccca agctcggcgc
    10861 aaactgaacg gcgtccattt ggtttagttg ccgcgggctt ggccctgctt gtgataggtg
    10921 ctggattcgc gttgataatc ccgctcatcg gattgacgct cgccgcattg ggagtggttc
    10981 tcgtcatatg gggtggagtg gtggggtggt ggatgacgcg aacagagagc gcgccggccg
    11041 gcggtcacgg acggcgataa ctcaagtagt ttctcattcc tttcgccccc ggcaatttta
    11101 agctgctgta ttgttttcag gacacgcccg gagtagtgtg tctcaattga gacacgaatt
    11161 gaattggcta actgaaacat agcagggcta tgcacggcac gtgttttgcg ccaacccaat
    11221 tgcagttatt tcttaacctc gtgcagctta tcccagcggc ttggctcggc gcggttgcat
    11281 tttaccaaag gaagaatggg tattcattga gatgagcgcg acgatcttgg tctgtgacga
    11341 cgattctgtg cctcggcgca atattgccag cgtcctggaa gaagagggtt ataccgttca
    11401 tcaagctgag gatggcaagg ctgctataga tgcggttaat cagatcgatt tcgatttggt
    11461 tttaactgat ttaaaaatgc ctggcgttga cggtctcagc gtgttgaagc acgtgcgcag
    11521 tatttcccca caaaccttgg tcattctaat gaccgccttt tcatcagtgg acaccgccgt
    11581 ggaggccttt cgtcttggcg cacaagacta cattctcaag cccatcgtca tcgaagatgt
    11641 gattcgcaag gtcggccgct ttctcgagca taaaaatctc gtatgggaag tcgagatgct
    11701 gcgccgagag attaatcgct catcggagcc gaccagcctg gtgggcagca gcaagccgat
    11761 tcaggatatt tttaaaattg tttcgaaagt cgcggcaacg aattctacgg ttttaattac
    11821 gggggagagc ggggtcggca aagaagtggt cgcccgaacg ttgcatttgc aaagcctgtc
    11881 caaggacaaa gtctttcttc cggtgaattg cagcgcgatt cccgacacgc tgctcgaaag
    11941 ccaactcttt gggcatgcca aaggcgcgtt caccggcgcc atcaacgctc aagagggcct
    12001 gtttcaaaaa gctcagggcg gcactatttt tctcgacgaa atcggtgaaa tgccgttgac
    12061 tctgcaaccc aagctcttgc gcgccattga aactaaagag gttctggctg tgggctcgac
    12121 ccagccggtc cgcttgaacg tgcgaattat cgcttcgacc aatcgggatc ttgaacgcga
    12181 agccaatgag ggtaagtttc gcgaagatct tttctaccga ttgaatgtcg tgcatgtctg
    12241 ggtgccgccg ctgagagaac gacgcgagga cattccgcta ctggtggact atcttatctc
    12301 gcgccacaac cgggagatga aaaaggcgta taagggggcg gacaattcgg caatgaaagt
    12361 tctgatgtct ctcccgtgga agggtaatat ccgcgagttg gacaacgtgc ttgagcgcgc
    12421 catgatcttg ggcgatggtg aatggattac gccggagcat ttgccggaat ccgaaattcc
    12481 cgaaaacgac cgaaatgtca cggccaataa tttgcgcgtc gcggtcgagt cctatgagaa
    12541 aagtcacatc gccaatgttc tcagagacac cgccggcgac cgtactcgag cagcggagct
    12601 ccttggattg agccgatcct cgctttatcg aaagatggaa aagctcaaca ttcgtgacga
    12661 ttaatcgact cccgccactc tcccgaaccg tttgcctggc catcgaatgc atccgtcaga
    12721 gcttacagct atctcaatca ccgccagctc cgttctgtcc gaaaaattag tccggcgatt
    12781 cgataacaac ttcgctgagt ggaatttaaa ttcctgttca ccgaaactgg cgtcgccgcg
    12841 gcgatcgcgc tcctgagatc aagttgtagg aaaagcatga cccggaaccg ccccgcaact
    12901 cgcaggcaaa aaaaacaaaa cgccggcact gccgttcgga ccccaaatcg atcggcagag
    12961 agcgctttaa gcagttcaac cgagccccgc atccgcgcca ttatcgaggg agtcaagccg
    13021 gaaatcgact gcggccggtt tccggtcaaa cgagtggtcg gcgatcgcgt caccgtcgaa
    13081 gccgatattt tcaccgacgg acacgacgcc ttgtcggcgc gcctgctctt tcgcgcgcag
    13141 ggcgatgccg actggaatga ggcgcggatg gagccgctgg tgaatgatcg ctggcgtggc
    13201 gtcttcgaca ttacggcgct gacggattat ttttacacgt tggaagcatg ggtcgatcgg
    13261 tttaaatcct ggcgtgacgg gttgagcaag aaagcagccg ccaaacagga cgtcggcttg
    13321 gaattgatcg aaggggcgga gctggttgcg ctggccgctc aacgggctca gggcggtgac
    13381 gccgagacat tgacagccta tgcggcagcg ctccgtgcca aagaacccgg cgcagtccag
    13441 aaagcgctgg aggaggagct ggccgcgctc atgagcaagc acgcggagcg gcgctgggcg
    13501 gcgcgttacg acaaggagct tcgtatcggt gtcgatcggc cgatggctga gttcagcagc
    13561 tggtacgaga tctttcctcg ctcctgcgcc gatcagccgc gcaagcacgg caccctgagg
    13621 gactgcatgg cgcgcctgcc gggcattgcc gcattaggat ttaacgtgct ttacctgccg
    13681 ccgattcatc cgatcggatg ggcctatcgc aagggcaaaa acggcacgct ctcgcccgaa
    13741 ccggatgatg tcggcagtcc ctgggccatc ggctcggaag agggcggtca taagtcggtg
    13801 cacccgttac tcggtaccct ggcggatttc aaacagctgg tggaaaaagc ccgagagcac
    13861 gagatcgaga ttgccctcga catcgcctat caatgctcgc ccgatcatcc ctatgttaaa
    13921 gaatatccgc agtggtttcg taaacggcct gacggcacca ttcaatacgc ggagaacccg
    13981 cccaagaagt atcaggacat ttatccaatc gattttgaga ctgaagactg ggacgctcta
    14041 tgggatgaat tgaagagagt tct
//
LOCUS       DASUVS010000421         9938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1280707, whole genome shotgun sequence.
ACCESSION   DASUVS010000421 DASUVS010000000
VERSION     DASUVS010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9938
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280707"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..3027
                     /gene="ppdK"
                     /locus_tag="VF182_22275"
     CDS             316..3027
                     /gene="ppdK"
                     /locus_tag="VF182_22275"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX6439861.1"
                     /translation="MAKNTKFVYFFGGGKAEGKASMRNILGGKGAGLHEMTRIGVPVP
                     PGFTITTDVCTHYYAHRGGYPKGLEIEVSRALKRLEKKLGKRFGDANDPLLVSVRSGA
                     RESMPGMMDTVLNLGLNDQTLQGLIHQTQDPRFGYDTYRRFVQMYSDVVLGVRARDKT
                     QVDPFEQIIADTKHDRGIHSDTELTADDLKEIVDRYKAAVKAETGKDFPDDPIAQLWG
                     AIGAVFSSWNNDRAVAYRELYHIPDEWGTAVNIQAMVFGNLGEACATGVAFTRNPSTG
                     EKKFYGEFLVNAQGEDVVAGVRTPRAIAELKSLMPKPYSQLQRVCITLEKHYRDVQDI
                     EFTIEDNNLWMLQTRSAKRTGFAAVRVAVEMVDEKIITKKEALLRIEADHLNQLLRPV
                     FDSHDKHKAALEQRVLVKGLPAGPGAATGRIVFHAEDAVESTRNGDRVILCRVETSPD
                     DIRGMEVAEGILTARGGMTSHAALVARQMGKVCVAGCEALHIDYGARVMRVNGAILRE
                     GDWISVDGSTGEVIKGELQTRPSEVLQAVLENTSPARKSPLYRQYTKLMKWADEFRSL
                     GVRTNTDQPDQARVARAFGAEGIGLCRTEHMFFQGDRIQAVREMILADDEAGRRKALA
                     KLLPMQKDDFKAILEIMGDLPVAIRTLDPPLHEFLPSREEEISELAHVMSVPLESLRD
                     KVKILQEFNPMLGHRGCRLGISYPEITEMQARAIFEAACELRREGKRSFPEVMIPLVG
                     SREEFAAQKNIVQRVAEQTMKSYGVKLRFLIGTMVELPRACLVADEIAQEAEFFSFGT
                     NDLTQTCLGLSRDDAGKFLPTYVLDGLLPEDPFVSVDQDGVGKLMEIAVAKGRKSRKN
                     LEIGICGEHGGDPKSIRFCHQIGLDYVSCSPYRIAIAKLAAAQAALL"
     gene            3159..4886
                     /gene="pilB"
                     /locus_tag="VF182_22280"
     CDS             3159..4886
                     /gene="pilB"
                     /locus_tag="VF182_22280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEX6439862.1"
                     /translation="MEPRVAELLVRGGVVSRDQLNKAQQKGRDSGSSVMKELVQLGFT
                     TEQALTEFLAKQFGIEKIELSPEEIEESVFTLVPPQLTQKHQLVPTKLSGSVLTVAMA
                     DPTDLIAINEIKFITGYGVRVVIASPSAIKKTLDHRFGGISYDDVLKKFGDSDMELIL
                     EQDDVNLQELQQATMEAPVVTLVNAILADAAKRRASDIHIEPYEKLFRVRFRIDGVLQ
                     EIMSPPLRLKNPLVSRLKVMAGLDIAERRLTQDGRIKLKMGIGGELDIRVSILPTLFG
                     EKVVMRLLDKSNLQLDMAKLGFDPQTLKDFQEAIHKPFGMILITGPTGSGKSTTLYSA
                     LSELNKADVNISTAEDPVEYNLVGINQVQVREQIGLNFASCLRSFLRQDPDIIMVGEI
                     RDLETAQIAVKAALTGHLVLSTLHTNDAPSTIDRLINMGVEPFLLTSSINLIAAQRLV
                     RRICDKCKEPIDVTPEALINLGVDASEAAGGFPTFRGRGCNSCNDTGYRGRLAIYEIL
                     VMHEGLRELILKSASAADLKREAVKLGMSTLRMSALQKVRDGFTTIEETIRVTDTDKG
                     FGSVFSLGF"
     gene            4923..6140
                     /locus_tag="VF182_22285"
     CDS             4923..6140
                     /locus_tag="VF182_22285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6439863.1"
                     /translation="MPTFLWEGKTAQGRLMKGDLEAPNLEAVFSALRERRIRPIPNRI
                     RQKGQGLDKEISIPGFGEKVKPKEVSLFTRQFATMIDAGLPIVQCLDILSEQSASKVL
                     RNTIRGIRQDVEGGLTLAASLKKYPKVFDDLYVNMVEAGEAGGVLNTILNRIALFIEK
                     ATKLKKKVKGAMIYPCSILAVAVIVVAVLLIFVIPVFAELFTSMGKALPVPTQIAINL
                     SNWFIAYWLYMIFGTAAFIGTLRFYYQTPQGRMHIDGILLRMPIFGDLLRKVAVARFS
                     QNMSILLSSGVPILDGLAITARTAGNKVVEKAIMDSRISISQGKTVSEPLRDSKIFPP
                     MVCQMVAVGESTGGLDGMLKKVGELYEEEVDDAVANLTALMEPLIMVVLGVILGALVI
                     SMYLPIFQMGSLA"
     gene            6200..8248
                     /locus_tag="VF182_22290"
     CDS             6200..8248
                     /locus_tag="VF182_22290"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6439864.1"
                     /translation="MAGDNKSLTEKIRWLLLLRLAILTFFLAATGLFNFFQAEGSPRF
                     FFIILKPLLIVYGISIGSLLILPRVRSLRLFGHAQNGFDVVLITGIIWITGDAASPFS
                     FLYNLAVMNAAILLFYRGAFFTAGFSSLCYAALLIWVIYFNEISGPPLSSSALVPVIL
                     NIGSFFVIAGLSGFLANKLGETEKLLEEKKNDYQELDALKEALMQGMVSGVAITDGKG
                     RINYFNLQAQNLTCLSEQAVIGKRLDEVFPTLRWNFDKPEPGPSIVLEEIVFASAKGD
                     QKNIRFSLASLNNSHEECMGYVSIFEDITRQKRFEEKVRVEEELRRAREFSLQELPLG
                     SSQEEFQFDGVIGTGGGIETIHRLIRKVALTRTNVLITGESGTGKELVARAIHLNGPR
                     KGRPFVAVNCGAIPEGLIESELFGHVRGAFTGAIADHPGLFKQADQGTIFLDEVGELP
                     VHLQVKLLRVLQETTFTPVGGSKPIKVDVRFIAATNRNLKREMEESRFREDLYYRLNV
                     VQIAIPALRDRREDIAMLAHFFMRKFASLHDKKIEEISSSALMHLLGHHYPGNVRELE
                     NIIEHAVTVTNKNILTDDDLPPYINANPAAEDCQSVETTAPDEINVFFEKGLSLDAEL
                     ETHERCILLGALKRANGVQKRAAEILGINYRSLRHRLEKYGMSGSRGSPLSDEFTEQQ
                     "
     gene            8570..8953
                     /locus_tag="VF182_22295"
     CDS             8570..8953
                     /locus_tag="VF182_22295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX6439865.1"
                     /translation="MKQLRLQNESGFTLIELLVVVAIIGILAAIAIPQFSAYRKRGYE
                     AAVKADLRNAATGQEAYFTTAFTYRAGVLTSGNLTGFNASPTVAVASGGGSNTYELTA
                     THQNCSTTVWTFSSGNGQISGGPCP"
     gene            9255..>9938
                     /locus_tag="VF182_22300"
     CDS             9255..>9938
                     /locus_tag="VF182_22300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439866.1"
                     /translation="MGMGFPLLANFQSAPFYPPHLVFLLVSFFDAIRILFVFHYLVSA
                     LGAYALGRQWGYAAYISVIAAFLFTFGGILISLTNVLNHFQAAVWLPWLVLTGERAIR
                     SYAGKDFLIFAGFGLVQFLAGSPELYALSLGLLCIHIVATVKECSAVTYVKAFLLVAA
                     ANVLLLAVSMIQLLPTIELFLQSRRPNNIPYQEATAWSLSPIGLLNFFFLDKEIDRSL
                     FNGVRLFFSH"
BASE COUNT         2476 a         2358 c         2613 g         2491 t
ORIGIN      
        1 gtgcgaacac actggtattt cagtctccgt gatggtgcga tactttgtgt cgattgttcc
       61 aagctcagaa aagaagttgt gcctatctcg ggtgcaactc tcgcatttat gatcagcctt
      121 cagcaggaag ggaatgttat cctggagaat ccaaagccgt cggcaacagt tctaaatcag
      181 attcgctctg ctacggtgcg gtttatacag tttcagctga atagggaaat caggtcggct
      241 tccttcctta atcagttctg ctcctgatca tgacctcttt accgacgaac tttcatttcc
      301 aacgaggtta ctgacatggc aaaaaacaca aaatttgttt acttcttcgg cggcggaaag
      361 gctgaaggta aggcatcgat gcgcaacata ctcggcggca agggggctgg gttgcacgaa
      421 atgacgcgca tcggagttcc agtgcctccc ggattcacca tcaccactga cgtttgtacg
      481 cattattacg cacatcgcgg tggttatccg aagggtctgg aaattgaggt tagccgggcg
      541 cttaagcggc tagagaaaaa actcggcaaa cgctttggcg atgctaatga cccattgttg
      601 gtatcggttc ggtctggggc aagagagtcg atgcctggta tgatggacac tgtgttgaac
      661 ttaggcctca atgaccagac gctacaaggt cttatccatc agacacaaga tccacgtttt
      721 ggctatgata cctatcggcg cttcgtccag atgtactcgg acgtcgtgct tggggtcaga
      781 gcgagagata agacgcaagt cgatcccttc gagcaaatca ttgcagatac aaaacacgat
      841 cgaggaatcc actcggatac tgagttgacc gccgatgatt tgaaggaaat cgtcgacaga
      901 tacaaggccg cagtcaaagc agaaaccggc aaggatttcc ccgatgatcc tatcgcacag
      961 ctttggggag ctattggcgc agtctttagc tcttggaaca acgaccgagc cgttgcatac
     1021 cgtgagctct accatatacc cgatgaatgg ggcacggcgg ttaatattca ggcgatggtt
     1081 tttggcaatc tcggtgaggc ttgcgccact ggagtggcgt ttacccgaaa tccgtcgacc
     1141 ggagagaaga agttttatgg cgaattcttg gtaaatgcgc aaggtgaaga tgtcgttgcc
     1201 ggtgttcgaa caccgagggc tattgccgaa ctaaaaagtc taatgccgaa accttactca
     1261 caattgcaga gagtctgcat aacgcttgag aagcattacc gagacgtgca ggacatcgaa
     1321 tttaccattg aggacaacaa cctgtggatg cttcaaacgc gctcggccaa acgcaccgga
     1381 ttcgccgctg tcagagttgc agtcgagatg gtcgacgaga agatcattac gaaaaaggaa
     1441 gctttgctgc gaattgaggc ggatcatttg aatcagcttc ttcgcccagt tttcgatagc
     1501 catgacaagc acaaggcagc gctagaacag cgcgttctgg ttaagggctt gccagcgggg
     1561 cccggcgcag ctactggccg aatcgtcttt catgcagagg acgcggtcga atcgacgaga
     1621 aatggcgacc gagtaatact ttgtcgggtg gaaacgagcc cagatgacat tcgcggcatg
     1681 gaggtggcgg agggaatttt aactgcccgc ggtggcatga cttcgcacgc cgcattagtg
     1741 gcccggcaaa tgggcaaagt atgtgtcgcc ggttgcgaag cgttgcatat cgattatggc
     1801 gcacgagtga tgcgtgtaaa tggagcgatt cttagggaag gagattggat ttcggtagat
     1861 ggcagcaccg gtgaagtcat caaaggtgag cttcagactc gtccctcgga ggttcttcaa
     1921 gcggtcttag aaaacacctc accggcgagg aaatccccat tataccgtca gtacactaaa
     1981 ctcatgaagt gggcagacga attccgcagc ttgggcgtgc gaactaacac cgatcagccc
     2041 gatcaagcca gagttgcgcg tgctttcgga gctgaaggga tcggtttgtg ccgaaccgaa
     2101 cacatgtttt ttcagggcga tcggatccaa gcggtgcgcg aaatgatcct ggcggatgat
     2161 gaagctggac gccggaaagc cttggctaag cttttgccga tgcaaaagga cgactttaaa
     2221 gcaattttgg aaatcatggg agacctgcca gtagctattc ggacactcga cccaccattg
     2281 catgagttcc tgcccagccg agaggaagaa atttcggagc tggcgcatgt catgagcgtg
     2341 ccgctggaat cgctacgaga caaggtcaag atccttcaag aattcaatcc catgcttggg
     2401 catcggggct gccgcttggg aatttcgtat cctgagatta ccgagatgca agcccgcgcg
     2461 atatttgagg ccgcgtgcga attgcgccgg gagggaaaga gatcatttcc tgaggtgatg
     2521 atcccgcttg tcgggtcgcg tgaagaattc gcggcacaaa agaatattgt ccaaagagtt
     2581 gccgagcaaa caatgaagtc ctacggcgtg aaactccgtt ttcttatcgg aaccatggtt
     2641 gagttaccgc gggcatgctt ggtagcggat gagatcgcac aggaagcgga attcttttcc
     2701 tttggtacca atgatcttac ccagacctgc cttggcctgt cacgtgatga tgccggaaaa
     2761 tttttaccca cctacgtgct tgatggactt ctgccggaag atccgttcgt cagtgtagat
     2821 caggatggtg tcggaaagct catggagatc gcggtcgcaa aagggcgaaa atcacgcaag
     2881 aaccttgaaa taggaatatg tggcgaacat ggcggtgatc cgaagtcgat tcgtttttgc
     2941 caccagatcg gtttggatta tgtcagctgc tcgccctatc gtattgccat cgcaaaacta
     3001 gcggccgctc aggcggcgct gctataaacc acctgatttc tgacatttag cagctgggtc
     3061 gcgcctctat aagccgttct cgtcgggaga agttttattg acaggtctct ctttattcac
     3121 cataatttca cgagccgaat ttccttcaaa tcatctctat ggaaccgagg gtagcagaac
     3181 tattggtccg agggggcgtt gtttcacgcg atcagttgaa caaagcccag caaaaaggac
     3241 gtgacagcgg ttcgtccgtg atgaaggagc ttgtccaact cggatttacc acagaacaag
     3301 ctcttaccga attccttgcc aagcagttcg gcatcgagaa gatcgagctg agtccggaag
     3361 agatcgaaga atcggttttc actctcgtgc ctccccagct tacccagaaa caccagctag
     3421 tcccaaccaa gctctccggc tcagttctta cagttgcgat ggctgacccc acggatctga
     3481 tagccatcaa cgagattaaa ttcatcaccg gctacggggt aagagtcgtg atagcatcgc
     3541 cctccgccat caagaaaacc ttagaccacc gctttggcgg aatcagttac gacgatgttc
     3601 tgaaaaaatt tggcgatagc gatatggagc tcatcctcga gcaggatgat gtcaatctcc
     3661 aagagcttca acaggcgacg atggaagcac ccgttgtcac tctggtgaat gccatcttag
     3721 cagatgcggc gaagcggaga gcgagtgaca ttcacataga accctatgaa aagcttttcc
     3781 gtgtaagatt ccgtatcgat ggggtgcttc aagaaataat gagccctccg ctgcggctca
     3841 aaaatccttt ggtctctcgc ctgaaggtga tggcgggact cgacatcgct gaacggcgac
     3901 ttacgcagga tggtcgcatt aaactaaaaa tgggtattgg cggcgagtta gacatccgtg
     3961 tgtctatttt gccgaccctc ttcggcgaaa aagtcgtgat gcgcttgcta gacaagtcta
     4021 atctccaact tgatatggcg aaacttggct tcgatcctca gacgctgaaa gatttccagg
     4081 aagccatcca taaaccgttc gggatgatcc ttataacggg ccccaccggc agcggcaaat
     4141 cgactactct ctactccgca ctttcggaat tgaacaaagc ggacgttaat atatcaaccg
     4201 ctgaagaccc ggtcgaatat aatcttgtag gcatcaacca agttcaggtc agagaacaga
     4261 taggactgaa cttcgcgtca tgtcttcgat cttttctacg gcaagatccg gacattatca
     4321 tggtcggtga aattcgtgat cttgagactg cgcaaattgc tgttaaagca gctttgacgg
     4381 gtcatctggt gctaagcacg cttcatacga acgatgctcc atccaccatt gatcggctga
     4441 tcaatatggg cgtggaaccg tttctactga cttcgtcaat caacttaatt gcagcgcaac
     4501 gcctggtgcg acggatctgc gataagtgca aagaacccat cgacgtcacg cctgaggcat
     4561 tgatcaattt gggtgtcgat gcttccgagg ctgcaggagg ttttccgact tttcgcgggc
     4621 gaggctgtaa tagctgtaat gataccggat atagaggtcg actcgcgatt tacgaaatac
     4681 tggtgatgca tgaaggtctc agagaattga ttttgaaatc tgcttcggct gctgatctga
     4741 aaagagaggc ggttaagttg ggcatgagca cgctgcgtat gagcgccctg cagaaggttc
     4801 gggacggttt cacaacgatc gaggaaacca tccgcgttac tgatacggat aagggcttcg
     4861 gctcagtttt ttcattagga ttctgagcgc acgtagacat acaccagaag gatttacaat
     4921 ccatgccaac gtttctatgg gaaggtaaaa ctgctcaagg aagactcatg aaaggggatc
     4981 tcgaagctcc caaccttgag gccgtatttt cagctttacg cgagcggcgg attcggccga
     5041 ttccgaaccg aatacggcaa aaggggcagg gtctcgacaa agaaataagc atcccgggct
     5101 ttggcgagaa agtaaaacct aaggaggtgt ctttattcac ccgtcaattt gccacgatga
     5161 tcgatgcggg tttgccgatt gttcaatgct tggacattct tagcgagcaa tcagcaagta
     5221 aagtgctccg caacacaatt cgtggaatca ggcaggacgt cgagggaggt ttgactttgg
     5281 cagcttctct taagaagtat cccaaggttt ttgacgacct atacgttaat atggtggaag
     5341 ccggggaagc cggcggtgtt cttaacacca ttctgaacag aatcgctctt ttcatcgaaa
     5401 aggcgaccaa actcaagaaa aaagttaaag gcgcgatgat ctatccgtgt tcaattctgg
     5461 ccgtcgcggt tatagtagtt gcggtactac tgatatttgt catccccgtt tttgctgaac
     5521 tgttcactag tatgggaaag gccctgccgg tcccgacaca gatcgccatc aatcttagca
     5581 attggtttat cgcttactgg ctctacatga tcttcggaac ggcggcgttt atcggaacgc
     5641 tccgattcta ttatcagaca ccgcagggca gaatgcacat cgatgggata ctgcttcgga
     5701 tgccgatttt cggggatctt ttgcgaaaag tcgcggtggc gcgattctcg cagaatatgt
     5761 ctattctcct gagttccggc gtacccattc tcgatggcct agcgattaca gctcggacag
     5821 caggcaacaa agtggttgag aaagcgatca tggacagccg tatcagtatt agccaaggta
     5881 aaactgtctc ggaacccttg cgagatagca aaatctttcc tcccatggtc tgtcagatgg
     5941 tggctgtagg cgaaagcacc ggcggcctcg atggcatgct gaaaaaggtc ggcgaactct
     6001 acgaggaaga agttgatgac gccgtcgcta atttgaccgc gctcatggag ccattgatca
     6061 tggttgtatt gggcgtgatt ctcggcgcat tggtcatttc gatgtaccta ccgatttttc
     6121 agatgggctc tttggcttag gcgctgaata gcggacctcc aacaggatca atacacccga
     6181 gaaccgtctg aagttctatt tggctggcga taataaaagc ttaacagaga aaataaggtg
     6241 gctgcttctt cttcggctag ctatcttaac gttctttcta gcagcaacgg gcctgttcaa
     6301 cttttttcaa gccgaaggga gcccgcggtt tttctttatc attctgaaac cgctgctaat
     6361 tgtttatgga atttctattg gctcgcttct gatcctgccg cgagttcgca gtcttcgatt
     6421 gtttggtcat gctcagaacg gctttgatgt agttctgatc accgggatca tctggattac
     6481 cggcgatgcc gcgagcccgt tctcgttcct ctataacctt gcggtgatga atgcagccat
     6541 attgctcttt taccggggcg cgttcttcac ggctgggttt tctagcttat gctacgcagc
     6601 ccttttgatt tgggtcattt acttcaacga gattagcgga cctcctttgt catcgtctgc
     6661 gttggtacct gtgattctga acatcgggag tttctttgtg attgccgggt tgagcgggtt
     6721 tttagccaac aagctcggcg agactgagaa attactcgaa gagaaaaaga acgactacca
     6781 ggaactggat gcactcaaag aagcgttgat gcaagggatg gttagtggcg tggcaatcac
     6841 tgacggtaaa ggaagaatca actacttcaa tctccaggct cagaatctta cttgtttgag
     6901 tgagcaggca gtcatcggca agcgactcga cgaagttttt ccgactctcc ggtggaattt
     6961 cgataaacca gaacctggcc caagtatcgt gttagaagaa atcgtcttcg ctagcgctaa
     7021 aggggaccag aaaaatattc gctttagctt agcctcatta aacaactctc acgaagagtg
     7081 tatgggatac gtttctattt ttgaagacat tacgagacaa aaacgattcg aagagaaggt
     7141 cagggtcgaa gaggaactcc ggcgagcgcg cgagtttagt ttgcaggaat tgccacttgg
     7201 tagctcgcaa gaggagtttc agttcgacgg cgtcattggg acgggcggcg gcattgaaac
     7261 tattcaccgg ctgatcagga aagttgccct cacccgaact aatgtgctga ttacgggcga
     7321 aagcggaacc ggcaaagaat tagtcgcgcg ggcaattcac cttaatggac ctagaaaagg
     7381 caggcccttt gtcgccgtga actgcggcgc gataccggaa ggtcttattg aaagcgaact
     7441 gttcggccac gtccgaggcg catttacagg ggccattgcc gatcacccag gactcttcaa
     7501 acaggcagac caaggaacca tctttcttga tgaagtaggc gagttgccgg ttcacttgca
     7561 agtcaaactg ctgagggtgt tgcaggaaac gacctttaca ccggttggtg gcagcaagcc
     7621 aatcaaagtc gatgtgcgct ttattgccgc gacaaaccgc aatttgaagc gggaaatgga
     7681 agagagtcgt tttcgtgagg acctttatta ccggctgaat gtggtgcaaa tagctatacc
     7741 cgccctgcgc gatagaagag aagacattgc gatgcttgcg cattttttta tgcggaagtt
     7801 tgcgagcttg catgacaaga agatagaaga aatttccagt agcgctctga tgcacctgtt
     7861 gggtcaccat tacccaggga acgtcagaga gctggagaat attatcgaac atgccgtaac
     7921 tgtgaccaac aagaacattc tgacggatga tgatttaccg ccgtacatca atgccaatcc
     7981 tgctgcagaa gactgtcagt cggttgaaac aactgcgccc gacgaaatca atgtgttttt
     8041 cgagaagggc ttgtccttag acgccgagtt ggaaacacat gagaggtgca ttctcctggg
     8101 cgcgcttaaa agagccaatg gcgttcagaa gcgagcagct gaaatcctgg ggatcaacta
     8161 tcggtcactc cgtcacagac tcgaaaagta tggtatgtca ggcagcagag gaagtccgct
     8221 atcggacgag tttacggaac agcagtaaca aattttgtca ctttgtcccc cgggtcaact
     8281 tataaatccc gaaatccgcc tactcctaat tgcctcctcg aacgccgctc cttcagtatt
     8341 gagtgattat ccggaaaatt gcgcacttca gtggcagggc ttgccttgat ccctgatgca
     8401 ggccggcccg gcctagatgg ctctgctttt gctcatcggc gagttcaacg ttcgaacgtc
     8461 aaaacagcac gaaggaggta tgcgagagaa ggcagtggtc aacttggtta catatctcga
     8521 agaaaattgt tgtaagtcca tttgaagtac gaaaggaaca aaacactcaa tgaagcagtt
     8581 acgcttacaa aatgaatccg gttttacact gatcgagctt ttagtcgtgg tagcgatcat
     8641 tggtatattg gccgccatcg ctattccgca gttttcggct tatcgaaaac gaggatatga
     8701 agccgcagtg aaagccgacc tgagaaatgc agctacaggc caagaggcct atttcacgac
     8761 agcgttcact tatagggcag gagttcttac tagtggcaac ttgacaggat ttaatgcaag
     8821 tccaaccgta gctgtggcat caggcggtgg atcaaacacg tatgaattga cggctactca
     8881 tcaaaactgt tcaaccacag tttggacctt ttccagtgga aacggccaaa tcagtggcgg
     8941 accctgtccg taactatccc gacttcaatc gtacattgat agatcactcc caggggaggg
     9001 gatacacatc ctctcccctt ttcatattca gagcgtggag actgcgtatc gctgctactg
     9061 aacgttgcga atatttattg gcgaattcga tcgcattgag ctgcatactc attgggatct
     9121 gttggttcag tcacgatatc atctgggagg ccgaggtacc tttttttcgg gatttgggtc
     9181 catatttcta tccgatccgg ttcagcttgg ctcagagtct cagcgccgga gagcttccac
     9241 tctgggatcg gcacatgggg atgggctttc cgcttcttgc caactttcaa tcggcgccgt
     9301 tttacccgcc acacttagtt tttcttttgg tttccttttt cgacgccatc aggattctgt
     9361 ttgtcttcca ctacctagtc tctgctcttg gagcgtatgc cttaggtcgg caatggggat
     9421 atgccgccta catttccgtt atagccgctt tcctcttcac gttcgggggc atccttatct
     9481 ctctgacgaa tgtgctgaat cactttcaag cagccgtatg gctgccgtgg ctggttctca
     9541 ccggagaacg ggcgatccga tcttatgcag gcaaagattt tttgattttt gctggcttcg
     9601 ggctagtgca atttttggcg ggttcaccag aactttatgc ccttagcctc gggttactat
     9661 gcattcacat tgtagcaacg gttaaagagt gctccgctgt cacgtacgtg aaggcgtttc
     9721 tactggtcgc cgctgctaat gttctgctct tggcggtgtc gatgattcag ctattgccga
     9781 cgattgaatt atttcttcag tcccggagac cgaataacat tccttatcaa gaagccacgg
     9841 cctggtcact tagcccaatc ggtctcctaa actttttctt tctcgataag gagatcgata
     9901 ggagcctgtt taacggtgtc cgattgttct ttagccac
//
LOCUS       DASUVS010000422         4578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_842869, whole genome shotgun sequence.
ACCESSION   DASUVS010000422 DASUVS010000000
VERSION     DASUVS010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842869"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VF182_22305"
     CDS             complement(<1..838)
                     /locus_tag="VF182_22305"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439867.1"
                     /translation="MGIRNILVGLVALSFLPAVTVHSMEKVVLGYSGVGSGEEVNHFA
                     KESGVFKKHGLDVEIVYIPGGSTVVQAMISGGVQFGRGSATEVVSSHLAGYPLKILAA
                     LINKFVYSFVTSLEISKPQDLKGKAVAVSRFGSGSDFITRMALKSWGLEPVRDVTILQ
                     IGNSPERLAAISSGKVHGSILSLSQTPRAKKLGLRVLADLSQIDAEYPQGVLYAPLSL
                     IEKRPDLVTTFVKAYVEAIHQFKTNRQLAYRIISRSSGLTDQADIEEYFNLLTGRFLL
                     DYP"
     gene            complement(847..1929)
                     /locus_tag="VF182_22310"
     CDS             complement(847..1929)
                     /locus_tag="VF182_22310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia lyase-activating protein"
                     /protein_id="HEX6439868.1"
                     /translation="MRSQSGYTQVAYGETAFDQFLDYEEIPVVRNFVVGDIRKLEVTP
                     WRRRGGLGCYIILGHPKEAPNSAAYVCEIPPGESLQPQKQMFEEMIFVLKGRGATSVW
                     LDNGKKQTFEWQERSLFSIPLNAWHQHFNGQGDESIRFIGYTNAPSVFNLYHNWDFVF
                     NNSYQFTDRFRGEDNYFSGKGVAIPDKRVWDTSFIADIEKLQFHDWTKKGPGATNVEL
                     EFCDNIISAHITRSPVGAYKKAHRHGLGADILVLEGKGYSLMWLEGTEMERYDWGPFS
                     MFSPPHMWFHQHFNTAKEPLKMLAFKPFGQKFKIFDYDKLFVSTSGGGHLIELEDEDP
                     KIRQMFKEEIESEGIPFNMPPVTYRR"
     gene            2080..2931
                     /locus_tag="VF182_22315"
     CDS             2080..2931
                     /locus_tag="VF182_22315"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6439869.1"
                     /translation="MKKIVVTGGIGRLGQLVIQELLMHDYQVLSLDRVRPAVNTCPSW
                     IADLRSPGDLYQALTGAQAVVHLGAYQAPGLAPDAEIFGNNVTASYNVLKAASDLGIK
                     KVVMASSTAAFGFIYALRRMVPEYLPLDEKHPSRPQDPYGLSKVVGEQIADSVALLHP
                     DMAISSLRFPGVIFDFSYDSVRQRWKDPGARANTSWAYIDGRDAATACRLAIEAEFAG
                     HEVMIVAAKTSSMREPTVELIEKYLGQGIQVKNARAGNWSGVSSAKAEKLLGFKARYG
                     WENFLTI"
     gene            3009..>4578
                     /locus_tag="VF182_22320"
     CDS             3009..>4578
                     /locus_tag="VF182_22320"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6439870.1"
                     /translation="MSYYKSLSEYVQILEREGLLIRIKKAINKDTEMHPLVRWQFRGL
                     GEEQRKAFLFENIYDAKGKQYDIPVLIGGLAASQRIYALGLMCKESEVEELWSQALEK
                     PIDPVFVKEGPVQEVVWQGEELIRRGGLYQLPIPISTPGFDNAPYLTAGCWITKDPET
                     GVRNMGVYRGQIKGPLKTGIMWGSLKHTAIHWEKCNERGIPLDAAIVLGGPPCITYAA
                     VQPVPYGVDEVALAGGLARCPLEMVGCKTVDLEVPAHADIVIEGKIPSDYLEPDGPFG
                     EAHGYLDPGDLNGVFEVTCITHRKNPIYVSMISQLTPSESSKIKQSAYETLALKHLNK
                     NVGVNATRVALYEDLLNRQVAVIQIKKEKPDDTWRALEGLLPSRVIHKLIVCVDEDID
                     PHDAQSLLWAIANRCQPHRDMRIVPNRPLPWNPIRYVADGEHYDVTDSTLLIDATLKA
                     AFPPVALPQQSFMEHARELWDELGLPKLTPRPPWSGYSLGLWPEEAAIQAGNAVAGNH
                     SLNAEFSSKHGIKVP"
BASE COUNT         1073 a         1209 c         1241 g         1055 t
ORIGIN      
        1 atgggtaatc caggaggaat cggccggtca ataaattgaa atattcttcg atgtcggctt
       61 gatccgtcag accgctgctc ctgctgataa ttcgataagc taactgcctg ttggttttaa
      121 actggtgaat tgcctcaacg taagccttta caaacgttgt gacgaggtcg ggtctctttt
      181 cgatcaaaga taacggcgca taaagaacgc cttgagggta ttccgcgtcg atctgcgata
      241 agtccgccaa aacgcgcagc ccaagtttct tggcacgagg agtttgtgag agggagagaa
      301 tggagccatg aacttttccg gacgagatcg ccgccaaccg ttccggagag ttgcctattt
      361 gtaaaattgt gacgtcccta accggctcca acccccagga tttcaaagcc atgcgggtga
      421 taaaatcaga gccggaacca aaccggctaa ctgccaccgc cttgcccttg aggtcctgag
      481 gcttagaaat ttccagggac gtgacgaagc tataaacgaa cttgttgata agagctgcca
      541 ggattttcag tggataaccg gctagatgcg agcttacgac ttctgtagca gagccgcgtc
      601 caaattggac accccccgaa atcatcgctt gaaccaccgt ggaaccgccg ggaatataga
      661 cgatctcgac atcgaggcca tgttttttaa atacaccgct ttccttggca aaatgattca
      721 cttcctcgcc cgagccaacg ccgctatatc cgaggacgac tttttccatg gagtggactg
      781 tgaccgcagg taaaaatgat agcgctacaa gcccgaccag tatgttgcga atgcccataa
      841 acctccttag cgccggtaag tcacgggcgg catgttaaac gggattcctt cgctttcgat
      901 ctcctcttta aacatttgcc gaatcttggg gtcttcatct tccagttcga tcaagtggcc
      961 tccgccgctg gtgctcacga ataatttgtc ataatcgaag atcttgaatt tctgtccgaa
     1021 cggcttgaag gcaagcatct tcagcggttc ttttgccgtg ttgaagtgct ggtgaaacca
     1081 catgtgcggg ggcgaaaaca tgctaaatgg tccccagtcg tatcgttcca tttccgtacc
     1141 ctcaagccac atcagcgagt accctttgcc ttcgagaacg agaatgtcgg cgccgagtcc
     1201 atgccggtgc gctttcttgt aggctcccac cggagagcgg gtgatgtgcg ccgagatgat
     1261 gttgtcgcag aattccagct cgacattcgt cgctcctgga ccctttttcg tccagtcatg
     1321 aaattggagc ttttcgatgt cggcgatgaa actggtatcc catacccgct tatcgggaat
     1381 tgcgactccc ttaccgctga agtaattatc ttcgccgcga aaccgatcgg taaactggta
     1441 actgttatta aaaacgaagt cccagttgtg atataagttg aataccgacg gcgcgttggt
     1501 atagccgata aatcggatcg attcatctcc ctgcccgtta aaatgctggt gccaagcgtt
     1561 gagaggaatg gaaaacaacg agcgctcctg ccactcgaac gtctgttttt tgccgttatc
     1621 gagccaaacg ctagttgcgc cgcgcccttt caaaacgaag atcatttctt caaacatttg
     1681 cttttgcggt tgcaggcttt ctcccggcgg aatctcgcac acataagccg ccgagttcgg
     1741 ggcctccttg ggatggccta agataatgta acagcccagc ccgccgcgcc ggcgccatgg
     1801 agtaacttcg agctttctga tatcaccaac gacgaagttt ctgacaaccg gaatttcttc
     1861 ataatcaagg aactgatcga aagcagtttc tccgtaggct acttgggtat aacctgattg
     1921 actccgcatg tttacctcct gcgcttcatc ggcattggct ggtgatcata ggttggcatt
     1981 taataaagtg tcaatttaaa agtatttgat gcacagttta atatttcttg agcaaaaatc
     2041 attgactcag ctcattcagc gaaggtaact aggagcgtcg tgaaaaaaat cgtggtaacc
     2101 ggtggcatcg ggcgcctggg acaactcgtc atccaggaac tgttaatgca cgactatcag
     2161 gttctttctt tggaccgagt gcggcccgcg gtgaacacgt gcccgagctg gatcgccgat
     2221 ttgcgcagtc ccggcgatct ctatcaggct ctcactggcg cgcaggcggt agtgcactta
     2281 ggcgcgtatc aagcgccggg tctggcgccc gatgcggaaa tattcggcaa taatgtgacg
     2341 gcaagctaca acgtgcttaa agccgccagc gatcttggca tcaaaaaagt ggtgatggcg
     2401 tcgagcacgg cggctttcgg gttcatttac gctttgcggc gcatggttcc ggaatacttg
     2461 ccgttggacg agaaacatcc gtcgcggccg caggatccgt atgggctgtc taaagtcgtc
     2521 ggcgagcaga tcgcagattc cgtagcgctg ctccacccgg acatggccat cagcagtttg
     2581 cgctttcccg gtgtgatctt cgatttctca tacgactcgg ttcgtcaacg ctggaaagat
     2641 cccggcgctc gcgccaacac ctcgtgggcc tacatcgacg gccgggatgc cgcaactgct
     2701 tgccgtttgg cgatcgaggc ggagtttgct ggccatgagg tcatgatcgt tgcggcgaag
     2761 acgagcagca tgcgcgagcc gactgtcgaa ttgatcgaga agtatctcgg ccaaggcatc
     2821 caggtgaaaa acgctcgtgc cggcaactgg agcggcgtta gctcggcaaa agccgagaaa
     2881 ctgctcggct tcaaagcccg gtacggttgg gaaaactttc taactattta agtcgtcgtc
     2941 ttaatcgtca tcggctgcta tcgattctat tactttcaag cttagagtat ccggccaaag
     3001 gattcgccat gtcttactac aaaagtcttt cagagtatgt gcaaattctc gagagagagg
     3061 gactgctgat ccggatcaag aaagccatca acaaagatac cgagatgcac ccgctggttc
     3121 ggtggcagtt ccgcgggctc ggcgaggagc aaagaaaggc tttcctgttt gaaaatatct
     3181 atgacgccaa aggcaagcag tatgacattc ccgttctcat cggcgggctt gcggcttcgc
     3241 agaggattta tgcgctgggc ttgatgtgca aagaatcgga agtcgaggag ctctggtcac
     3301 aagcgttgga gaagcccatc gacccagttt tcgtaaaaga aggcccggta caggaggtcg
     3361 tctggcaagg cgaagagctg attcgcaggg gaggccttta tcaactgccg atcccgatat
     3421 ccacgccggg ctttgacaat gcgccttatc taaccgccgg atgctggatc accaaggatc
     3481 cggaaaccgg ggtcagaaac atgggtgtgt accgcgggca aatcaaaggc ccgctcaaga
     3541 ccggcatcat gtggggcagc ctcaagcata ccgcgattca ttgggagaaa tgcaacgaga
     3601 ggggaattcc gctcgacgcc gccatcgttc ttggcggccc gccctgcatt acttatgcag
     3661 cggtgcagcc ggttccctac ggcgtcgatg aagtggcgct ggcaggcggg ttggcgcgct
     3721 gcccactgga gatggttggc tgcaaaacag tcgatctcga ggttccggcg catgccgata
     3781 tcgtaatcga agggaaaatt ccttcggatt atctcgagcc cgacgggccg tttggcgagg
     3841 cgcacggtta tttggatccg ggagatctaa atggcgtatt cgaagtcacc tgcatcaccc
     3901 atcgcaaaaa tcccatttac gtttctatga tcagccagct gacgccgagc gaatcgagca
     3961 agatcaagca gtccgcctac gagactctgg cattgaagca tctcaacaaa aacgtcggag
     4021 tgaatgccac ccgggtcgcg ctctacgaag atctgctcaa ccgccaggtc gcggtgatac
     4081 agatcaaaaa agaaaagccc gatgacacgt ggcgcgccct cgaaggactg ctgccatcgc
     4141 gggtgattca caagctgatc gtctgcgtcg atgaagacat tgatccgcac gatgcccagt
     4201 ctcttctttg ggcgatagca aatcgctgcc agccacaccg cgacatgcgg atcgtgccga
     4261 accgccccct gccgtggaac ccgattcgct atgtcgctga tggggagcat tatgatgtga
     4321 cggattcgac attgctgata gacgcgactt tgaaggcggc gtttcctccg gtggcgctgc
     4381 cgcagcaatc cttcatggaa cacgcgcgcg agctttggga cgagttaggc ttgccgaaat
     4441 tgactccgcg cccgccctgg tccggatatt cattgggctt atggccggag gaagcggcaa
     4501 tccaggccgg caacgcagtc gccggcaatc acagcctgaa tgccgagttc agttcgaagc
     4561 acggtatcaa agtgccca
//
LOCUS       DASUVS010000423         3554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1060114, whole genome shotgun sequence.
ACCESSION   DASUVS010000423 DASUVS010000000
VERSION     DASUVS010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060114"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VF182_22325"
     CDS             <1..366
                     /locus_tag="VF182_22325"
                     /inference="COORDINATES: protein motif:HMM:NF013062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEX6439871.1"
                     /translation="FMPTTDLPDGDPRKDLVFIDDDMVRLRARVERLKSRHGHREYPS
                     SRTRRFISNIRIKEEEGGIVATSSFIVYRFRMGESSPYVGWYEHRLRRVNEGLKIHHR
                     KAVLDLEALSDHGAVSIIL"
     gene            789..1646
                     /locus_tag="VF182_22330"
     CDS             789..1646
                     /locus_tag="VF182_22330"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6439872.1"
                     /translation="MGKITANIHQLTDSVFAVIGIDGATNFGIIKGRDHSAVLIDADI
                     RRMDEIDDALERTECKKVRYLVNTHENFDHSSANDYFEKNGTVVVGTNGCYRALEEDG
                     DQKFAEMAGRSPELWKRFPGLKMANLQITFPQEIMLHLPGVAVRLLFAAHNGQSHSRG
                     DAIAMLQQDKILFAGDLLYTDFHPVTVYGNIPNWLQSINHLLGHDFVSVVPGHGPVSS
                     GGNDVYKSAFEKFRSYLEDFDGRLKEMKTGKKSPGEVESYMKSGAYASMGKTWMVKRN
                     IEYSLRARN"
     gene            1649..2626
                     /locus_tag="VF182_22335"
     CDS             1649..2626
                     /locus_tag="VF182_22335"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439873.1"
                     /translation="MLRLFLSLLVIAYLAGSSVSVSAADKLRVGFPSLATALSPTWVA
                     AKKGFWKKHDLDVELILLSGAVIIPTLLSGSLDVVIGSDTSAIISNLKGGNNLRLGVT
                     TNSLGSTLVTQRSVDSIKQLKGKIIGIGSQGFSSLELRLSKLLLDNGIDPEKEVKFLP
                     IGGNPSARVAALENGLVIAAMITPPYDRVAVESGMKSLAKIDAPVIAGGINVRSDYLQ
                     GNRDKLVRFLKGYIEGIHFLINNKKETVKIFSDYLGARDPKVIEYFYGEIAGRVEKSL
                     SPDPESVRFTLDFIGRLNPKAKEIKTTDFSDLSLLEEIQQSGFIHKLYR"
     gene            complement(2694..>3554)
                     /locus_tag="VF182_22340"
     CDS             complement(2694..>3554)
                     /locus_tag="VF182_22340"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439874.1"
                     /translation="HDQGVFKRYGFDVEYLALNSGTLGAQTLLSNEVQFLFSTGALAV
                     TANLHGGDMTMVSGGFNFFAFKLVARPEIKNGEDLKGKRFAISQFGSATDFAVQASLE
                     KLGINPKDATLIQLGGNPSRIAALTGGSADASVFSEPVATLAIKKHRMNLTLDMAAAG
                     LAFPQSALMVKRSYLEANRDKVTSFLKAMIEGLYIVKKDKALAIRLTKKYIRAEDEMY
                     AVGYDYFLGQYGEDLLNMPDRKGLELVIAQTAKTNPKAKGQTPESLRLMDASVLEEIK
                     KSGFVEKLKR"
BASE COUNT          864 a          874 c          941 g          875 t
ORIGIN      
        1 ttcatgccga ccaccgatct tcccgacgga gatccccgca aggatctggt gtttatcgat
       61 gatgacatgg tacggctgcg cgctcgagtg gaaagactga agagccgcca tggtcataga
      121 gagtatcctt cctcgcgtac ccggcgcttt atttccaata tccggatcaa agaagaagag
      181 ggcggcatcg tcgcgacttc ctcgtttatc gtctatcgtt tccgcatggg cgaatcgagc
      241 ccgtatgtcg gttggtacga gcacagacta agacgagtaa acgaagggtt gaagattcat
      301 cataggaaag ccgttctgga cttggaagcg ctgagcgatc acggagcggt gagcatcatt
      361 ctttagcgcc cggcggcaag gattgcgttc gagcagttcc aaaccttcca ttcgttccat
      421 tgtctccccg ccttaaatac gatgaactcg tttaaggaca gtgaacgtgt tgaaatcgtt
      481 ccagccattc aatcgctgtg ctccgctcaa atcgttctga gaattgccat caaacttcag
      541 acacttgtgt cggatcatag ccggatgacg attgcatttc agcagttcag atgttccatt
      601 cgcccctaaa acacaataga cctgctccag cggagcgcag cggttcggcc agtgaaacac
      661 tctgagatgg tttaacagat aagtaagtac agggagtccg cgtaaatgcc gggcggacca
      721 cgccgcgtca tttcgtctta acgttaagaa cttaccgtgc tatccactgc tcagggaggc
      781 aggcaatcat gggaaaaatt actgcgaaca tacaccagct tacagactcg gtatttgccg
      841 tgatcggcat cgacggcgcg acgaactttg gcattatcaa aggtcgcgat cattcggcgg
      901 tattgatcga tgccgatatc cggcgcatgg acgagatcga tgacgctctt gaaaggacag
      961 agtgcaaaaa agttcgttat ctggtgaaca cccacgagaa ttttgatcac agctccgcca
     1021 atgactattt cgagaaaaac gggaccgtcg tggttggcac gaatggctgc tatcgggcgc
     1081 tggaggaaga tggggaccaa aaatttgccg agatggcagg gcgatcgccg gagctgtgga
     1141 agaggtttcc cggattgaag atggccaacc tgcaaatcac gtttccgcaa gagatcatgc
     1201 ttcatcttcc gggcgtggcc gtacggctac tttttgcagc ccataacggc cagagccata
     1261 gccgcggaga cgcgatagcg atgctccagc aggacaaaat tttattcgcc ggcgatctcc
     1321 tttatacgga ttttcacccg gtcaccgtat acggcaatat tcccaattgg ctccaaagca
     1381 tcaatcacct gcttggccac gattttgttt ccgtggtgcc tggacatggg ccggtatcga
     1441 gcggcggcaa cgacgtttac aaatcggcct tcgagaagtt tcgcagctat ctggaggatt
     1501 tcgacggccg gcttaaagaa atgaagacag gtaaaaaaag ccccggtgaa gtcgaatcgt
     1561 atatgaagag cggcgcatac gcctccatgg gtaaaacctg gatggtcaaa cgcaatatcg
     1621 aatattcctt gagagcgagg aattaactat gttgcgtctt tttctttcgc tgctagtgat
     1681 cgcttatctt gccggctcat ccgtcagcgt ttctgccgcc gacaaacttc gggttggctt
     1741 tccctctctg gcgactgcgt tgagtcctac atgggtggca gcaaagaaag gcttttggaa
     1801 gaaacacgat ctcgatgtcg agctcatctt gctatcgggc gcggtgatta ttccgacgtt
     1861 actgagcgga agtttggatg ttgtcatcgg ttcagatact tcggcaataa tttcgaactt
     1921 aaaaggaggg aataatctgc gcctgggcgt gactaccaac tcactgggtt ccaccctggt
     1981 gacgcagcga agtgtcgact cgatcaagca attaaaaggc aagataatcg gcattgggag
     2041 ccaggggttc agctcgctgg aactgcgcct gtcgaagctg ctgctggaca atggcatcga
     2101 tccggaaaaa gaagtcaagt ttttgcctat cggaggcaac ccaagcgctc gagtcgcggc
     2161 tctggagaac gggctggtga tcgctgcgat gatcactccg ccctatgata gggttgcggt
     2221 agagagcggc atgaagtcgc tagcgaaaat tgatgctccc gttatcgccg gcgggatcaa
     2281 cgtcagaagc gattatctgc aaggcaatag ggacaaactc gttcgttttt tgaaaggcta
     2341 tatcgaagga attcatttcc tgattaacaa taaaaaggaa accgtcaaga ttttttccga
     2401 ttatctgggt gcccgtgatc ccaaggtgat cgaatatttt tatggagaaa ttgctgggcg
     2461 ggtggagaag agcctgagtc cggatccgga gtcggtgcgc tttaccctcg acttcattgg
     2521 acgcttgaat ccaaaagcga aggagatcaa gaccacggat ttttcggatc tgtccttgtt
     2581 ggaagagatt cagcagtccg gatttatcca taaattgtat cgataaatac gagcggcaat
     2641 tctaaaacgt aatgactgga tctggattcg tgctagggac atgcgttcaa tgatcacctc
     2701 ttcagcttct cgacgaaccc gctttttttg atttcttcga ggacgctcgc gtccatcaat
     2761 cggagcgatt cgggggtttg tccctttgct tttgggttgg tcttagccgt ttgcgcaatt
     2821 accagttcga ggcccttgcg atcgggcatg tttaaaagat cctcgccgta ttgccctaag
     2881 aaataatcat agccgacagc atacatttca tcttcggccc ggatgtattt tttcgtgagt
     2941 cgaatggcca atgctttatc cttcttgacg atatagagac cttcgatcat tgccttcaag
     3001 aacgaggtga ccttgtcacg gttcgcttcc aggtaacttc tcttgaccat cagggcgctt
     3061 tgtggaaacg ccagacccgc cgctgccatg tcgagggtga gattcatgcg atgtttctta
     3121 atcgccagcg tggcgaccgg ctcgctgaac accgaggcat cggcagaccc gccggtcagt
     3181 gctgctatgc gactgggatt gccgcccagt tgaatgagcg ttgcatcctt cggattgatt
     3241 ccaagtttct ccaaactcgc ttgaacagca aaatcggtgg ccgagccgaa ctggcttatg
     3301 gcaaaccgtt ttcctttcag atcctccccg ttcttgatct ccgggcgagc gaccagcttg
     3361 aaagcaaaaa aattaaagcc gccggaaacc atggtcatat caccgccgtg caaattggcg
     3421 gtaacggcca aagcaccggt agagaaaaga aactggactt cattggagag tagagtttgt
     3481 gcccccaacg tcccgctgtt taaagctagg tattcgacgt cgaagccgta tcttttaaac
     3541 acgccttgat cgtg
//
LOCUS       DASUVS010000424         8075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1499839, whole genome shotgun sequence.
ACCESSION   DASUVS010000424 DASUVS010000000
VERSION     DASUVS010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8075
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499839"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /gene="recO"
                     /locus_tag="VF182_22345"
     CDS             complement(<1..513)
                     /gene="recO"
                     /locus_tag="VF182_22345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX6439875.1"
                     /translation="MQTSHFTSAIVLRSWPFGESDKIVSFLTERHGKLTGIAKGAKRS
                     KKRFVNSLEPFSFVNLNFQDRSRSSLAFILGCELQKSYKNLIASLEKIAFASYYVEIT
                     EGLLGEREQNRLVFDHLKKGLNRLEIEEASVTFLIDFELKLLSLTGYQPFLDSCRRCG
                     MQPSLSYEPVR"
     gene            658..1266
                     /gene="grpE"
                     /locus_tag="VF182_22350"
     CDS             658..1266
                     /gene="grpE"
                     /locus_tag="VF182_22350"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX6439876.1"
                     /translation="MSEESKKPEHWSDSAESMVDSASERQDEQAELRDKFQAKEKESQ
                     ENYERYLRQVAEMENFKKRVNREKEEAIRHANENLIKDILPVIDNLERAIAHAQGGGN
                     GKPLVEGVEMVLRGLLDVFSKYGVVQVPAVGVVFDPGKHEAMAQVESSEHEPNTIINE
                     HHKGYIFRDRLLRPALVTIAKAPQDKDGKNGQIEVEKGPADD"
     gene            1315..3231
                     /gene="dnaK"
                     /locus_tag="VF182_22355"
     CDS             1315..3231
                     /gene="dnaK"
                     /locus_tag="VF182_22355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX6439877.1"
                     /translation="MAKVIGIDLGTTNSCVAILEGGDPNVLANSEGSRTTPSVVAFTD
                     SGERLTGQIARRQAITNPENTIFAVKRLIGRRFDDLMVQRAAKVLPYKVVRNDNGDAW
                     VEIRGKQYSPAEISAFILQKMKQTAEDYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLNVLRIINEPTAASLAYGLDKKKDEKIAVFDLGGGTFDVSVLELGEGVFEVKATNG
                     DTFLGGEDFDQRIIDYLADEFKKDQGIDLRKDRMALQRLKEAGEKAKCELSSSMETDI
                     NLPFITADQQGPKHLNMKLTRSKLEALCGDLLDRLEAPCRQALKDAGLSAGDIHEVVL
                     VGGMTRMPAVQERVKKIFGKEPNKGVNPDEVVAVGAAIQGAVLTGDVKDVLLLDVTPL
                     SLGIETLGGVFTKLIERNTTIPTKKSQVFSTAQDNQTAVSIRVFQGEREMAADNKLLG
                     QFDLVGIPMAPRGVPQVEVTFDIDANGIVHVAAKDLGTGKEQSIKITASSGLNEEEIK
                     RMVSDAESHASEDKKRKETVEARNQLDTLIYSTEKSLKEHGGDLEPGVKENIESALTQ
                     AKAALEGQDAQAMKTAADQLSQSSHKLAESMYAKASQQQKTQDTGETEGQSSEDGSKK
                     KEDVVDADFTEVKE"
     gene            3350..4462
                     /gene="dnaJ"
                     /locus_tag="VF182_22360"
     CDS             3350..4462
                     /gene="dnaJ"
                     /locus_tag="VF182_22360"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX6439878.1"
                     /translation="MNGKKDYYELLGVGRNASEDEIKKAYRKLALQHHPDRNPGDKQA
                     EETFKSVSEAYQVLSDSHKRAQYDQYGHAAFGDSGPFAGGFDFNSGFEDIFGDIFGEF
                     FGGAGGRRRAQTRGEDLRYNLSLSFEEAVAGVEKKIKIPRHGPCETCRGTGAKPGTSP
                     QTCPTCHGRGQVSFQQGFFSVSRSCNQCHGQGTIIADPCASCGGGGRTRKMHTLSVKI
                     PAGVDNGSRLKLRGEGESAPSGGAPGDLYVVIQVETHPIFRRENLDILCDVPITFVQA
                     ALGAEIDVPTLEGKVKMKVPQGTQSGKLFRIKGKGVKDPRGYQQGDQHVRVVVETPTH
                     LTARQKELLKEFASLGGEDVNPLAKGFFDKVKQLFD"
     gene            4579..7071
                     /gene="lon"
                     /locus_tag="VF182_22365"
     CDS             4579..7071
                     /gene="lon"
                     /locus_tag="VF182_22365"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX6439879.1"
                     /translation="MSDTEWKVEDSEGKGSQVEIPEVLPLLPIRDIVIYPYMMLPLFV
                     GRDVSIRAVEESLSRDRLIFLVAQKNSSEESPNPSEIHKVGTIASIMRMLKLADGRVK
                     ILVQGLSKGEVDIFTRERPFFEVKIRKVIEPTLQEVPIEVEALMRTAKEKIEQILNLK
                     NLPPEIVMVTDNISDPGVLADLVASNLRLKIDESQAILEIFDPIERLKKVNDLLSREL
                     ELSTMQARIQNQAKEEMSKTQRDYFLREQLKQIQQELGEGDERAEEMNEVRKQIDKAK
                     MPPEVKKEADKQLRRLEQMHPESSEASLVRTYLDWLVDLPWSKKTKDNLNIKKAKQVL
                     DEDHYNLEKIKERILEYLAVNKLRKKLKGPILCFVGPPGVGKTSLGRSIARALGRNFV
                     RVSLGGVRDEAEIRGHRRTYVGALPGRIIQGIKQAGSNNPVFMLDEIDKVGADFRGDP
                     SAALLEVLDPEQNHAFSDHYLNLPFDLSNTLFICTANLLDPVPAALADRMEVIQLSGY
                     TNEEKLEIARKYLIPRQFEDNGLSAKTIEISDDAMLRIIGEYTKEAGLRNLEREIASI
                     CRKVARKVAEGKTELTRITRANVHSFLGAPKFLPEAEQEHHEIGVTTGLAWTSAGGEI
                     LYVEASLSKGRGNLTLTGQLGEVMKESAQAAVSYARAHAKKLDIEEDFYQKLDIHIHV
                     PAGAIPKDGPSAGITMATALISALTKRAVSRDVAMTGEITLRGRVLPIGGLKEKALAA
                     FRAGIKTIIIPERNEKDLDEIPKVLRRKLQWVLAESMSDVLKNALLDKPSPKKLAKGA
                     GDRVRARRARPSKRGAKPLQKTRRDRSIPLRP"
     gene            7165..>8075
                     /gene="thiL"
                     /locus_tag="VF182_22370"
     CDS             7165..>8075
                     /gene="thiL"
                     /locus_tag="VF182_22370"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX6439880.1"
                     /translation="MKLSRLGEFGLIERVRRTMPTSRDVALGIGDDAAWVRAKTDSFL
                     VTADLLLEDVHFNLNWTSLSDLGYKTLAVNLSDIAAMGGVPAYLTLSLGIPSHFTSEE
                     MDRFYHGIKSLAVKHNVTVVGGDTSVADKLLISACLIGHAPYRPIRRSGAKLKNDIYV
                     TGTLGDSALGLQLLKRGMLTQKGAGYLLSRHHKPTPRLAVGALLARKKLATAMIDVSD
                     GLLQDLGHICRASHLGARIWEEALPLSGAYRELAGNEGTHYALSGGEDYELLFCARPS
                     DRQLIDRLQHQVRVPLTRVGSCVPANQG"
BASE COUNT         2212 a         1895 c         2175 g         1793 t
ORIGIN      
        1 tcgcaccggt tcgtatgaca gtgatggttg cataccacag cggcggcaac tatctagaaa
       61 cggctggtac cccgtcaaac tgagaagctt taattcaaaa tcgataagaa aagttacaga
      121 tgcttcttca atttctagac ggtttaggcc cttcttcaaa tgatcaaaaa ccaatctatt
      181 ctgttcgcgc tcgccgagga gcccctccgt gatctcaaca taataagagg caaaggcaat
      241 tttttctaag ctcgcgatca aatttttata gcttttctgc aattcgcaac cgagaataaa
      301 tgctagactg cttctagatc ggtcctgaaa gtttaaatta acgaaggaga atggttcgag
      361 ggagtttaca aaccgcttct ttgaacgctt agctcctttg gcaatcccgg tgagtttgcc
      421 gtggcgctca gttaaaaatg agacaatctt gtctgattct ccgaaaggcc aactacgtaa
      481 tacgatagcc gaggtaaaat ggctggtttg catggatgct tcgcgccgga actaccttac
      541 catcgattct aatgttcgac aactattcaa taaatctacg ctttctaaat cgggccaact
      601 tgacaaggtt gagagccgtg attagatttg cgctattatt ctgtgggaga ctttagaatg
      661 tccgaagaaa gcaaaaaacc agagcactgg agtgactccg ccgaaagcat ggtcgactcc
      721 gcgtcagaaa ggcaggatga gcaagcggag ttgcgtgaca aattccaagc caaggagaaa
      781 gaatcacaag agaattacga gcgctatctc agacaagtgg ccgaaatgga gaacttcaaa
      841 aagagagtca atcgagaaaa ggaagaagcc attcgccacg ctaatgaaaa cttgatcaaa
      901 gatattcttc cggtgatcga taacctcgaa cgggcgatcg cgcatgctca aggtggcggc
      961 aatggtaagc cattggttga aggggttgag atggtattaa gaggactgtt ggacgttttt
     1021 agtaaatatg gtgtggtgca ggtcccggcc gtaggagtgg ttttcgatcc aggtaaacac
     1081 gaagcgatgg cccaggttga aagcagtgag catgagccta ataccattat caatgaacac
     1141 cataaaggtt atatctttcg cgaccgatta ctgcgtcctg cactggtcac gattgctaaa
     1201 gctccacaag ataaagacgg aaaaaacggt caaatcgagg ttgaaaaggg tccggccgat
     1261 gattaaatca aaactggccc gcaacgttta agacttcatt taggagcaac tgaaatggca
     1321 aaagttattg gaatcgactt aggcacaacc aactcctgtg tcgcaatttt ggagggtgga
     1381 gaccctaatg tcttggcaaa ttcagaggga agccggacta cgccgtcggt cgtggcattt
     1441 accgatagcg gagaacggct gacaggacaa attgcaagaa gacaagccat tacgaaccct
     1501 gaaaacacca tcttcgcggt caaacggcta ataggcaggc gtttcgatga tcttatggtg
     1561 caaagggcag cgaaagttct cccctataag gtagtccgca atgacaacgg agatgcctgg
     1621 gtcgagattc gcggcaaaca gtatagcccc gctgagattt ccgctttcat cttgcaaaag
     1681 atgaaacaaa cagcggaaga ttacctcggc gaaaaggtaa ctgaggcggt tattacagta
     1741 ccggcgtact tcaatgacag ccagagacaa gcaaccaagg atgccgggcg tatcgccgga
     1801 ttaaacgttc tgcggatcat aaatgaaccc actgctgcgt ctcttgctta cggactggac
     1861 aagaagaaag acgagaaaat cgccgttttc gatcttggcg gtgggacttt cgatgtgtcg
     1921 gtcctggaac taggtgaggg cgtctttgaa gtcaaagcta cgaatggtga tacctttctc
     1981 ggcggtgagg atttcgatca aagaatcatc gattatttgg ctgatgagtt caagaaggat
     2041 caaggaattg atttaagaaa ggatcggatg gccttgcagc gccttaaaga agcgggtgaa
     2101 aaggccaaat gcgagctatc ttcctcgatg gaaaccgaca tcaatctacc gtttatcaca
     2161 gcagatcagc aaggtccgaa acatctcaac atgaagttga cgcgatcgaa gcttgaagcg
     2221 ctttgcggcg atttgctcga tcgactcgaa gctccctgtc gccaggcatt gaaagatgct
     2281 ggtctgagcg ccggtgacat tcatgaagtg gtactggttg gcggtatgac ccggatgcct
     2341 gcagtgcagg agcgagtcaa aaagattttt ggaaaagagc cgaataaggg cgtcaatccc
     2401 gatgaggtcg ttgctgtagg tgcggctatt caaggagcgg ttttgaccgg cgatgttaag
     2461 gacgtcttgc tgctggatgt cacaccgctt tcacttggaa tcgagaccct tggcggagta
     2521 ttcacaaaac tcattgagcg aaacaccacg atcccgacta aaaaaagcca ggtattttcc
     2581 accgcgcagg ataatcaaac cgcagtttcc atcagggttt ttcaaggcga gcgggaaatg
     2641 gcagcggaca acaagctcct gggacagttc gatcttgtgg gtatcccgat ggctccccgc
     2701 ggggttcctc aggttgaagt aactttcgac atagacgcga acggtatcgt tcatgtcgca
     2761 gccaaggatt taggaacagg gaaagaacaa tcgattaaaa tcacggcttc aagcggactg
     2821 aacgaagaag aaattaagcg catggtttcc gatgcggaat cgcacgcgtc cgaggataag
     2881 aagcgtaaag agactgtgga ggctcgtaat caactcgata cgctcatcta ctctacagaa
     2941 aaatcgctaa aagagcatgg cggcgacctc gagccgggag tgaaggaaaa tattgagtcg
     3001 gcgctaactc aagccaaggc agccttggaa ggccaggatg cccaagcaat gaagactgcg
     3061 gcggatcagc tgagccaatc atctcacaaa ctggcggaat cgatgtacgc taaagcgtcg
     3121 cagcagcaaa agactcagga taccggtgaa acagaaggac aaagcagcga ggacggaagt
     3181 aaaaagaaag aggatgtggt cgacgcggac ttcaccgagg ttaaagaata atctccaatc
     3241 tggtctaagt ggtcactaac aagtcgtcgg gaaaagcccc tgctccgatc actagcgatg
     3301 gagaggggct ttccttcatc tacaaatgta atcaaaaacg aggcggcgct tgaacggcaa
     3361 aaaagattac tatgagctgc tgggagtcgg tcgcaatgcc agcgaggatg agatcaagaa
     3421 ggcgtaccga aaacttgcgc tgcagcatca tcctgatcga aaccccggag acaaacaggc
     3481 ggaagaaacc tttaaatcgg tttccgaggc gtaccaggta ctttcggact cgcacaagcg
     3541 agcacagtac gatcagtacg gtcatgcggc tttcggtgat agcggtccgt ttgctggcgg
     3601 attcgacttc aattctggtt tcgaagatat ctttggcgat atctttggcg aatttttcgg
     3661 cggagcgggt ggacgccgcc gcgcccaaac gcgcggagaa gacctacgtt acaatctaag
     3721 cttaagcttc gaagaagcag tcgctggggt agaaaaaaaa atcaagattc cacgccacgg
     3781 accgtgcgag acctgtcgtg gtactggcgc caaacctggg acatcgccgc agacgtgtcc
     3841 cacctgtcat ggccgcggac aagttagctt tcaacaaggt tttttcagcg tctcgcgcag
     3901 ttgcaatcaa tgtcatgggc aagggactat aatagctgat ccatgcgcat cttgcggcgg
     3961 aggtgggcgc actcgcaaaa tgcatacgtt gagtgtaaag attccggccg gtgttgataa
     4021 cggttctcgg cttaagcttc gcggcgaagg ggaaagcgcc ccaagcggcg gggcgcccgg
     4081 agatctatac gttgtgattc aggtagagac tcacccgatc ttcagacgtg agaaccttga
     4141 tatactttgc gatgtgccaa taacttttgt gcaggcagcg ctaggggcgg agattgatgt
     4201 ccccacgttg gagggcaagg tcaaaatgaa ggtaccccag gggacccaat caggaaagct
     4261 ctttcggata aaagggaagg gtgtaaagga cccgcgcggg tatcagcaag gggatcaaca
     4321 cgtccgtgta gtggttgaga ccccgaccca ccttacagca aggcagaagg aacttcttaa
     4381 ggagtttgcc tccttaggtg gagaagacgt taacccgctt gcgaaaggtt ttttcgacaa
     4441 agtgaaacag ttgttcgact agctcgcttc ccagacaaac ttctttggtt agcccttgca
     4501 gcgcatcctg ggcaaagtaa aattgttgtt gacgtgggtt ttcagtctga ctaagttgag
     4561 caaggaatat aacacttgat gagtgacact gaatggaaag ttgaagactc ggagggtaag
     4621 ggatcccaag tagaaatacc tgaagttctc ccgctactgc cgatccgcga tatcgtcatc
     4681 tatccctata tgatgctgcc gctcttcgtt ggccgagatg tttcgatccg agccgttgag
     4741 gagtctcttt ctcgcgaccg attaattttt ctggtagcgc agaagaattc ttccgaagaa
     4801 agcccaaatc cgtcagaaat acacaaggtt ggcacaatcg cttccatcat gagaatgctg
     4861 aagctcgccg atggacgggt caagattttg gtccagggtc tatccaaggg agaagtggat
     4921 attttcaccc gtgagcgtcc gttttttgag gtaaagattc ggaaagtcat cgaacctacg
     4981 ctacaagaag ttcccatcga agttgaagct ttgatgcgca cagccaaaga aaaaatcgag
     5041 caaattctga acttaaaaaa cctccctcct gagatagtga tggtgaccga caacatcagt
     5101 gatcccggcg tactggcgga tctggttgcc tctaaccttc gtctgaagat cgatgagagc
     5161 caagcgatcc tcgagatttt cgatcccatc gagcgactga agaaagtcaa cgatctttta
     5221 agtcgcgaac tagaactgtc gacgatgcag gcgcgtatcc agaatcaagc caaagaagag
     5281 atgagtaaga ctcagcgcga ttattttttg cgcgagcaac tgaaacagat acaacaggaa
     5341 cttggtgaag gcgacgaacg cgccgaagag atgaatgagg tccgaaaaca aatcgataaa
     5401 gctaaaatgc cccctgaagt taagaaggag gcggacaagc agcttcgcag gttagagcag
     5461 atgcatccgg aatcatccga ggcctctctg gttcgaacct atttggattg gctggttgac
     5521 cttccgtgga gcaaaaaaac caaagacaat ctaaacatta aaaaagccaa gcaggtcctc
     5581 gacgaggatc actataactt ggaaaagatc aaggagcgca tcctcgagta cctcgcggtc
     5641 aacaaacttc gcaagaagtt gaaaggaccg atcctctgct ttgtcggacc cccgggtgtc
     5701 ggcaagacgt ccctgggccg ttctatcgca cgggctcttg gccgcaactt cgtgcgggtt
     5761 tctttgggag gcgtacgcga cgaggcggag atccgtggtc atagacgcac ctatgtggga
     5821 gcattaccgg gccgcatcat tcaaggtatc aagcaggccg gctccaataa cccggttttt
     5881 atgttggacg aaatcgataa ggtaggggcc gatttccgtg gcgatccctc ggccgctcta
     5941 cttgaggtgc tcgatccgga gcagaaccac gccttcagtg atcattatct gaacctgccg
     6001 ttcgatcttt caaacacgct gtttatttgt actgccaatc tgctggatcc tgttccagcg
     6061 gctttagcgg atcggatgga agtgatccag ctctcgggtt ataccaacga ggagaaactc
     6121 gagattgccc ggaaatatct tattcctaga caattcgaag ataatggact ttcagcgaag
     6181 acaatcgaaa tttccgatga tgccatgttg cgcatcatcg gcgaatacac caaggaagcg
     6241 ggtctgagaa atctcgaaag ggagatcgca tcaatctgcc gaaaggttgc ccgtaaggtg
     6301 gccgaaggaa aaaccgagtt gacccggatt acacgtgcga acgtccattc cttcttagga
     6361 gctccgaagt ttctacctga agccgagcag gagcaccatg agatcggggt tactaccggt
     6421 ctggcttgga ccagcgcagg cggcgaaatt ctctatgtcg aagcttcgct ctccaaggga
     6481 cggggcaact taactctgac cgggcaattg ggcgaagtta tgaaggagtc agctcaagcg
     6541 gcggtgagct atgctcgagc gcatgctaaa aagctggata tcgaagagga tttttatcaa
     6601 aagctcgaca ttcatatcca tgttccagca ggggcgatcc ccaaggatgg accgtcagcg
     6661 ggcattacga tggcaactgc gctcatttct gcactcacga aacgcgctgt tagtagagat
     6721 gtggctatga cgggagaaat caccctacga ggtagagtgc tacccatcgg cgggcttaag
     6781 gaaaaagcgc tagcggcttt tcgagccggg ataaaaacga ttatcattcc cgaacgaaat
     6841 gaaaaggact tggatgaaat tcccaaggtc ctacgccgca aacttcagtg ggtgcttgca
     6901 gaaagcatgt ccgacgttct aaagaacgcg ttactggata aacctagccc gaagaagttg
     6961 gcaaaaggcg cgggcgacag ggtgcgggct cgccgtgcta ggccatcgaa acgcggtgca
     7021 aaaccgctgc agaaaactcg tcgagatcga tccattccct tgcggccata accaattcta
     7081 ctgaactcaa ccgagcaaac gctctcaaac gctgcacccc gtgccggttc tcttacttga
     7141 tcgcgcatga tagcgactct tgctatgaag ttaagccgtt tgggtgaatt cggcctgatt
     7201 gaacgggttc gacgcacaat gccaacgagt cgcgatgtag cgctcggcat cggcgatgat
     7261 gcggcctggg tcagagccaa aaccgattcc tttcttgtca cggcagatct cttgctcgag
     7321 gacgttcatt tcaatcttaa ctggacttca ctatccgatt taggttacaa aaccctcgcg
     7381 gtcaatttga gcgacatcgc tgccatgggc ggggtcccgg cctatctaac cctttctctg
     7441 ggtattccaa gccattttac cagcgaggaa atggaccgtt tttaccatgg gatcaaatcg
     7501 ttggcagtga agcacaatgt gaccgtcgtc ggaggagaca ccagtgtggc cgacaagttg
     7561 ctgatttccg cttgccttat cggtcatgcc ccctatcggc cgatccgtcg cagcggcgcc
     7621 aaactcaaga acgatatata tgtcacggga accttggggg attcagcgtt gggtctgcaa
     7681 ttacttaagc gcgggatgct cacacaaaaa ggcgctggat atctccttag ccggcatcac
     7741 aaacccacac cgagacttgc tgtaggtgct ctgctggcgc gaaaaaaatt ggctactgcg
     7801 atgatcgatg tgagtgatgg gctgctgcaa gacctcgggc acatttgtag agccagccac
     7861 ctaggcgcgc gcatctggga agaggctttg ccgttatcag gagcgtaccg agagctagct
     7921 ggcaatgagg gcacacatta tgcgttaagc ggtggtgagg attacgagtt attgttttgc
     7981 gctcgcccga gtgatcgaca gctaattgac cgattgcagc atcaggttag agtgccgtta
     8041 acccgagtcg gctcctgcgt cccggctaat caagg
//
LOCUS       DASUVS010000425        17028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_843888, whole genome shotgun sequence.
ACCESSION   DASUVS010000425 DASUVS010000000
VERSION     DASUVS010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 17028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17028
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843888"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="VF182_22375"
     CDS             complement(<1..1134)
                     /locus_tag="VF182_22375"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6439881.1"
                     /translation="MYEQHFGFKESPFSITPDPRFFYANSVYLEAYANLRYGIEAKKG
                     FIAITGEVGTGKTTLLRKLMLSLDKTIQTVLVFNTDVTFNELLRVILRELGLPRAGKD
                     RLGMVEELNDYLIEQLERGQTVCLLMDEVQHLSDESLEGLRLLSNLETDRQKLLQIVL
                     MGQPELQDKLDQPHLRQLKQRIAIRCEIAPLRDDEVGSYINFRMRVAGCDDPDLFHAD
                     AIDEIAFYSKGIPRLINVICDNALVIAFAGSQKSISRDVIGEVIRDLRLTPANQPTQN
                     PINETAAGAAIRSIETTPQISKRKPMNWFTAAAPASLAIVILAGFASLTDPGTFLLDT
                     GRFLFGSQSQLTKGTLANTGQNTGPTKASAKIAAAPMQTAQINT"
     gene            1683..1943
                     /locus_tag="VF182_22380"
     CDS             1683..1943
                     /locus_tag="VF182_22380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439882.1"
                     /translation="MAERYRNHLILVKADFDSYSRFWNVRAHIQFNQHVTFRDVLITG
                     PKETFRSQKRAETHIIQEAKKWVDNRLREIANKAQTADTVET"
     gene            complement(2697..3122)
                     /locus_tag="VF182_22385"
     CDS             complement(2697..3122)
                     /locus_tag="VF182_22385"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX6439883.1"
                     /translation="MTKAILTVVFGLITWWCAGAADRDNYASGARQEVSRGKYLVENV
                     AMCVQCHTPRSQTGELLETRYLQGGPVPVSAPPNFRIDWAYKAPAIAGLPGYTMQDGV
                     KLLMEGITPDGRTPKPPMPRFRLTRSDAEAVVNYLKSLS"
     gene            3427..4386
                     /locus_tag="VF182_22390"
     CDS             3427..4386
                     /locus_tag="VF182_22390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008633111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX6439884.1"
                     /translation="MRAMVLPRFGGSDLFEMHELECPEAGYGEVLVRVIASAVNPVDA
                     KLRANGAWAKLDPPVILGYDVAGVVEQVGPRLSDFRVGDEVYYTPEIFGNPHGSYAEY
                     NVASAAIVARKPAGLSFVEASAIPLAGGTAWEAVIRRLAIRPGETILIHGGAGGVGSF
                     AIQFAKAAGARVIASASADNQDLLRQLGADVTIDYRKDDAAEITLRETYGYGADAAFD
                     IEGADIVSRCLPAVRPFGRIACILPPQGDLSALYQKNITLHGTFLTRERRRLDEMRPL
                     FERKQAHPVIDRVLALEEVGKAHDRLDAHHGRGKIVLHVRSDF"
     gene            4470..6893
                     /locus_tag="VF182_22395"
     CDS             4470..6893
                     /locus_tag="VF182_22395"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX6439885.1"
                     /translation="MRIQFRRKLRKLLALGVLIATLPGIPAEANQATPHAPSAAWTES
                     TPINTPSPAAIPLPEIATEAEAALARVRDRIAEVPDNRTMPAVVQQVPLLTREIDARL
                     RQTRRILAQRPSIQTLSYLESEWRPLRRNLAAWNQQLTGYLSRLEGEIAEFDELSKVW
                     RETLESANAANVPREVLIRIQAVLDETEKARKEIEGYRGRALTLQNRVAAQDGRIADA
                     LSLIAETREEALDRLFTPDSAPIWSIDLRSYTAEVLRAQSSNSVATQWQALSAYAARQ
                     TPSFLLHAGIFIGMAAGLLWLGRRVQPRLEDTGIARTTIVFETPVAAGFLVSIFFSRW
                     IYPQAPPRLFWAFLGVIALIPTAVILRKLIARDLYPALYGLATFYVVDQVRAILIAEQ
                     VVARLLLFGETLGLMAFLVWLLRSIDRLRSQNTTFDRLSKIVNLAAVLALIMAGAGLI
                     ANAFGYSTLASLLGNTLLSSGYFALILYALVELIDSFVVIAVSLRPFRTLGIIARHGA
                     LLRRRIYRILQWAAVLLWIVHMLNGLLLRQRLFRAVSDFVNEELVVGSLRISLSDIVV
                     FGVTVWAAVLVSRFVRFVLDEEIFPRVHLTRGLPYAISSMVNYAIVLVGFFAAVAALG
                     VDMTRVTILAGAFSVGVGIGLQNIFNNFVSGLILLFERPIKVGDIVQIDDASGVVHRI
                     GIRASIIGTPSGAQVIVPNGKLISERVINWTFSNRGRSIELQLAVAPVADPRRVIALL
                     ERAANEHPHISRDPAPKAWVVKLGPDSLGLELRAWTDRIHDWMQVRSELAIAVSDNLA
                     AENIALRAV"
     gene            complement(7000..7320)
                     /locus_tag="VF182_22400"
     CDS             complement(7000..7320)
                     /locus_tag="VF182_22400"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX6439886.1"
                     /translation="MWYIVISRSLPDKEAEKQSHYEEHRQWLDNQHTAGRLLFSGPTT
                     DGRFGIYIMLASSLKDAEGLAAKDPHHVRGIREMQVLEWRVHRAFQLNGPTIEAVEGF
                     AKKA"
     gene            7688..8257
                     /locus_tag="VF182_22405"
     CDS             7688..8257
                     /locus_tag="VF182_22405"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX6439887.1"
                     /translation="MVKMKDHPSRVSASYADNDGIKVALFTPALDEWKETCMNRVEIT
                     GNRNHDKSLGSLISGIFQDAQTLIGKEIAAAKLELKQEMSKGIKAGVSLGIGGFLLVV
                     GFILLTLMIVFLISTYTPIPLWGSLGIVGLVYCVAGAIALIIGKRKVTEVKPVPEETL
                     QHTKEDVRYIAERASSGLEKGEIRKATQH"
     gene            8523..9944
                     /locus_tag="VF182_22410"
     CDS             8523..9944
                     /locus_tag="VF182_22410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013924590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6439888.1"
                     /translation="MAQAQRYDAVVVGSGPNGLAAAITMVRSGRSVAVYEAAEKVGGG
                     MRSAELTLPGFVHDVCSAVYPLAIGSPLFRSLPLDEYGLKWVQPPAALAHPLDDGSAV
                     LLERSLDHTAAGLGRDGNAYRRLMRPLVNSWQDLDSDLLGPLRWPRKPVAMARFGLSA
                     IRPARSFSESWFEEQPARALFAGLSAHSMLPLEAWGSAAFGLVLAITGHALGWPLARR
                     GSQSLAEALGLYLRSKGGEIYTNAPVRTLDELPSCRVILCDVTPKQLLALAGDRLSPS
                     YRRRLQRYRYGMGAFKIDWALSAPVPWKAHACARAATVHLGGRLQEIAASERAAWRGE
                     HTTEPFVLAVQPSLFDSSRAPETKHTLWTYCHVPNGSHFDMAARIEAQIERFAPGFGD
                     TVLARSVAGPAELERRNSNLIGGDINGGAPTLSQLFLRPTLKLYSTSAPGLYLCSAST
                     PPGGGVHGMCGYFAARQALRDCF"
     gene            complement(10184..11263)
                     /locus_tag="VF182_22415"
     CDS             complement(10184..11263)
                     /locus_tag="VF182_22415"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6439889.1"
                     /translation="MRSSSHSKLSLPVKIAAAGVAAAAFSYTLDRYRRIDFSGKTVLI
                     SGASRGLGLELARRFAAQRANVVLLARDQERLIEAARELKQYGVEISTRQCDVTQAQE
                     ARRAVTSVIDEFKSIDVLVNNAGIIQVGPAENMNRDDYADALAVHFWAPLNLTAEVLP
                     YMKGQGSGRIVNITSIGGKVAVPHLAPYVASKFALVGFSEAMRAELIKDGIYITTVVP
                     GLMRTGSHINAFFKGQHQKEFALFSIANASPLFSTASQRAARQIVEACRYGKAELIIT
                     PQARLLHLANSLFPNITAEVLGLISRALPSTRSGEGNALKRGWQSHSFMAPSVLTRTA
                     DRVIRRNQEEPRSAPHSNASNGYLK"
     gene            11491..11748
                     /locus_tag="VF182_22420"
     CDS             11491..11748
                     /locus_tag="VF182_22420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439890.1"
                     /translation="METIKIDDPRLDESFAFMFSLRKGDAVLLKSQNSLRGKVTDGVY
                     VGDFPKHVAGDIHPRGVTLYEISLADDTIQILEEKELQKVD"
     gene            12067..12462
                     /locus_tag="VF182_22425"
     CDS             12067..12462
                     /locus_tag="VF182_22425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439891.1"
                     /translation="MPLILSLSSVGCESIALMPRPAIDDPADVSRRDLDRGDLDRRPE
                     NRADIPRSKTADEVTGTVQKIDQNRREIQLRTTEGRMTVIKYDPATVVYNREREMPVD
                     DLRYGDLILIKIVKNNRGEQYADLIRLAS"
     gene            12676..14205
                     /locus_tag="VF182_22430"
     CDS             12676..14205
                     /locus_tag="VF182_22430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HEX6439892.1"
                     /translation="MLPSCPFRRLSHIICCNLLVLLAFLFECRLAAGNGVFGLDQVAE
                     TARKLAAEPFKPPAPVPDFLKQISYDDFRDIRFDTDQSLWKDGGNFQVQFIHPGLYYT
                     HSVVINTIDLQGIRKVAFSPKQFSYGKNKFADKIPPELGFAGFRIAYPLYKKNEYNHV
                     IVFAGASYFRAVAKKQGFGLSARGLAIDTALPSGEEFPTFREFWLERPTAQARALKIF
                     ALLDSQSLTGAYEFIVRPGDRTTVDVKARLFERKRVKELGVAPLTSMFLYGEEKPRPD
                     TDWRPEVHDSDGLMIASGSGEWIWRPLINPVRLQVSYFELDNLRGFGLLQRDRSYSNY
                     EDLETRHEMRPNAWITPSGTWPKGQIKLVEIPSQKETNDNIVAYWIPRSMPAVGQPLD
                     LAYRINFQTEEPLDAVSGRVTATRVGVGDKEDLKRIILDFEGAKLKSLPDTAPVKVVV
                     TVGADGQLVQQSTVKNPITGGWRAAFQVKPPKDKPLQVRAFLKNDTDTLTETWSYLLE
                     P"
     gene            14295..16655
                     /gene="mdoH"
                     /locus_tag="VF182_22435"
     CDS             14295..16655
                     /gene="mdoH"
                     /locus_tag="VF182_22435"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEX6439893.1"
                     /translation="MTTNGGAVADAPSGPGPATIRELNRHTRQWGKAERRQRLVQSDL
                     NSVRENIHAWAMPEIKRLSMLPNRMERNPLVTLWRALRGRTRNNDIERTGPASTKAAL
                     AGWRGAAASRRRVLIALIIIQTAIATWSLGNTFPYPWLKASEIAILVLFAILFSWISL
                     GFWSAVAGFWVVWKRKNEFTGADLPVDDRQPLRSRTAVLMPICNEDVIRVFAGLEASY
                     RSLAETSQFASFDFFVLSDTGDPERQAEEEIAWAQLCAAVQGFGRIFYRHRRNNVRRK
                     SGNIADFLRRWGRDYDYMVVFDADSIMAGETVVRLAQMMEQYPQAGIIQTAPTTVNCD
                     SLFGRLQQFASRAYGPVLTAGQRFWQLGDGYYWGHNAIIRTAPFIKHCGLARLPGRAP
                     LGGEILSHDFVEAALMGRAGWEVWVVHDLGGSYEESPPTLSDELKRDRRWCQGNLQHL
                     RLLLADGIRAGHRSILAMGVMAYASALLWFVFLILNTVELAAQTVLPLVYFTSEPSLF
                     PLWPRWHPEWAVALLTTTAVLLFLPKILSLLVIVKNRQTHLFGGFLPLCVSICLEIIV
                     STLLAPIRMWFHSQFVVVTLMGRQIKWGPQFRTDNETRWRDATRLHGIATLLAFGWVF
                     GMFWLNPAVSVWLLPVGISLLLAVPLSVFSSRLSLGRLMRQWRLFLIPEEIRSPQVIN
                     YLHASLIKRRENRQRDGFVQAVVDPYANAVHLGLLRGKKPQTSEAIDRNRNLRELALR
                     NGPAILSRADKTRLLRDSETMALLHRQVWRVRDTNLANQWGLSELQ"
BASE COUNT         3768 a         4381 c         4760 g         4119 t
ORIGIN      
        1 ggtattgatt tgggctgttt gcatcggcgc agcggctatt ttcgcagatg ctttagttgg
       61 gccggtgttt tgaccggtat tcgctagagt tcctttcgtt agttgactct gggacccaaa
      121 taaaaacctg ccggtgtcca ataaaaatgt gccgggatcg gtgagcgaag caaagccggc
      181 caggatgacg atcgctaaag aggcgggtgc ggcagctgta aaccaattca tcggcttgcg
      241 cttggagatc tgaggtgtcg tttcaatcga gcgtattgca gcgccagccg ccgtctcgtt
      301 gattgggttt tgggtgggct ggttggccgg cgtcagccgc agatcgcgaa tcacttcccc
      361 gatgacatct cgagagatac ttttctggga gccggcaaat gcgatcacta aggcattgtc
      421 acaaattaca ttgattagcc gcgggatacc cttcgaataa aaggcaattt cgtcaatggc
      481 atcagcgtga aataggtcag gatcgtcaca gccggcgacc cgcatccgaa aattgatgta
      541 agaccctacc tcgtcatctc tcaatggagc gatttcacac cgaatagcga ttcgctgctt
      601 gagctgcctt aggtgcggct ggtcgagttt gtcctggagt tcaggctggc ccatcagtac
      661 gatctgcaaa agcttttggc gatctgtttc gagattagag agaagccgca atccttccaa
      721 cgattcatcg ctcagatgct gaacttcatc catgagcaag caaaccgttt ggccgcgctc
      781 aagttgctcg attagataat cgttcagttc ctctaccatg ccaaggcgat cttttcctgc
      841 tcttggtaaa ccgagttcgc gcaggataac ccgcagcagc tcgttgaacg taacgtcagt
      901 gttgaaaacg agcaccgttt gaatggtctt atccaagctg agcatcagct tgcgcaacag
      961 tgtcgttttt ccggttccga cctcgccggt aatagctata aagccctttt ttgcctcgat
     1021 accatagcga aggtttgcat atgcttccag gtaaacggaa ttggcgtaaa aaaaacgtgg
     1081 atccggcgtt atgctgaagg gggattcttt aaatccaaag tgttgttcgt acatgcagta
     1141 agctatagag ttgttagttg tttaccgatg cttctgttca aggctcttaa ttcctgcgta
     1201 accaggtcca ccctagcgta gctgctgcca cggtaggacc gctcacctag agtacaatgc
     1261 agctatcggt tggccacttg cgtgtactaa cctgacctgc agcccctttt atcgcggcga
     1321 tcgagactca gcaacctttg tgcctgctcc gaaatggggc ctgattactc gctgcttaga
     1381 gcatcaggcg cggcgatttt gggccgagaa gcggcaaact cgcctgcccg catggacgaa
     1441 tcgggttgat cgggtttata cgtcgaggag ccagacagcg gatcaagaac cggatagcta
     1501 gattgttcgc agagtttatc gaggtgctcg cgcacgtctc cgaccgtgtt gacaatgtag
     1561 cgccggttga cttttagcgt gatcgggtgt ttttaggatc ggttttctgt agcccggaac
     1621 taactaatag tgtagataga agaagctaat ctaagcccat atgcgatcat gagaggataa
     1681 gcatggccga acggtaccgc aaccatttga ttctcgtcaa agctgatttt gactcatatt
     1741 cgcgcttctg gaatgtcaga gcgcatattc aattcaacca gcacgtcacc tttcgtgacg
     1801 ttctaataac cggccctaag gagacgttta ggagtcagaa gcgggcggaa acgcatatca
     1861 tccaagaagc gaaaaagtgg gttgacaata ggctccgcga gattgccaat aaggctcaaa
     1921 ccgcagatac agtggagacc taattggttc gcaattgcct ttggctagca caatgcgagc
     1981 gaatattcac gtagcgtgag atctccttcg gaggactcct gtgggtgctt agtatccaaa
     2041 tggcattgcc atgctactct gaagcgaact cgatctcgga gcggagaata gaaactgttt
     2101 caacttcggc aatcctgaca tgttgctggc agcgatttac catgaacgaa atactctgtg
     2161 accactgtga agggcctctg acaggcattg cttatcacgt aataagcgaa gaagatggcg
     2221 tcatactact ggatatggtg gtctgccacg gttgctatct ggaggcgaga aggattggac
     2281 tcgagaccgc tgagcttggg tcggtcattc agcaagtccc gggacgcagc taggcaatag
     2341 cgtacccaga ttagggacca aactcacacc caccatctgc cggctttatt caattatctg
     2401 ccgatctctg ctttgcgata ccagcgcgct cccgtttcag gatttaaaat cgtttgcctc
     2461 ggcgttccga caggatagat gacgttcaac gtgaacaagc gtggagcaga tgccgagaat
     2521 aggtatttga gctgccttgt tgcttaatat gtaacattac cccacaccgt tgtggacgtg
     2581 gagcgcgctg catatttgag gattactccg tacaaatcgg aaccagtcga tttgaatagc
     2641 taggggctgt cggatcgaac agccgcttga tatcgcatga tacggctagc atgcatttac
     2701 gacagggatt ttagatagtt tacgacagcc tcagcgtcgc ttcgtgtcaa gcgaaacctg
     2761 ggcatcggtg gttttggggt tcttccgtca ggtgtgattc cttccattaa taatttgaca
     2821 ccgtcttgca tcgtatagcc gggcaggccg gcgatggctg gagccttgta cgcccagtca
     2881 attctaaaat tgggcggcgc tgaaaccggc acgggtccgc cctgtagata tcgcgtttcc
     2941 agcaactcgc ctgtttgact ccgcggcgta tggcactgaa cgcacattgc aacattctcg
     3001 accaggtatt tcccgcgcga gacttcctgc cgcgctccgc tggcgtagtt atcgcgatcg
     3061 gcggccccgg cgcaccacca ggtgatcaaa ccaaacacta cagttaaaat agctttcgtc
     3121 acagatccct cctcgcgtcc gttgtaaggc gcaacgttgt gtgccgctga ttagatgatc
     3181 tcaccacgta gcatgattac tccgatcgtg gaatacatca attgcagtta tcgatcagca
     3241 ctgcctcgga tgtctcaaag ttgagatgat cgtgaaataa tcgtgatcca acgtttccgc
     3301 ggatgccgag aattacgccg aatggtatat cgattgcgtt attgctgctc ctgcatgacg
     3361 cgaggctcaa accaattgag tgtcttctta aaagagtatt gatagagagg cgaaaggatc
     3421 gaaatcatgc gcgcaatggt tctgccacgg tttggtggat cggatttatt cgagatgcat
     3481 gaactagaat gtcctgaagc cggctacggt gaagttctgg ttcgtgtcat cgcttcggca
     3541 gtcaatccgg tggatgcaaa actccgcgct aatggcgcct gggcaaaatt ggatcctccg
     3601 gtgatattag ggtatgacgt tgccggtgtc gtggaacagg ttggaccgag gttaagcgat
     3661 ttcagagtgg gtgatgaggt ctactacact ccagaaattt ttggcaaccc tcacgggagt
     3721 tatgctgaat ataatgtagc atccgctgcg atcgtcgctc gcaaacccgc cggtctgtca
     3781 tttgtggaag ccagtgcaat tccgcttgct ggaggcacgg catgggaagc ggtcatccgc
     3841 agattggcga ttcggcccgg cgaaaccatt ctgattcatg ggggcgcggg gggagtgggc
     3901 tcctttgcca ttcaatttgc caaagccgca ggcgcgagag tgattgccag cgccagcgct
     3961 gacaaccagg atttattacg gcagctgggt gccgacgtga ccatcgatta tcgcaaagat
     4021 gatgcagccg aaatcaccct gagagaaacc tacggttatg gcgcggatgc cgcatttgat
     4081 atcgagggag ccgatattgt ttcgcgctgt ttgccggccg ttcgaccgtt cggtcggatt
     4141 gcctgtatct tgccgccgca aggcgactta tctgcactgt atcaaaaaaa catcacgctg
     4201 cacggcacgt ttctgacccg agagcgccgc cgattggacg agatgcgccc gctgtttgaa
     4261 agaaaacaag cccaccctgt gatcgaccgg gtgcttgctc tagaggaagt gggcaaggcg
     4321 catgaccgtc tcgatgcaca tcatgggaga ggaaaaattg ttttgcatgt gagaagcgat
     4381 ttttaatctc tctgtaatcg caattccaaa agcatagcgt gctgaggaag cggcgcgcat
     4441 gtgcgtggca aatacgtgac cgagatctca tgcgaattca atttagacga aaacttcgaa
     4501 agttgctggc actcggcgtg ctgatcgcta cactgccggg gattcctgct gaagcgaacc
     4561 aagcaactcc tcatgcccca agtgcggcgt ggacggagtc aactcccatc aacacaccga
     4621 gcccggcagc gattccatta cccgagatcg ctactgaggc cgaagcagcc ttggcgcgag
     4681 tgcgcgaccg tatcgccgag gtgcctgaca atcgaacgat gccggccgtg gtacagcagg
     4741 tcccgctatt gacccgggag atcgatgcgc gtcttcgtca gacccgcagg atattggcgc
     4801 agcgaccgtc gatccagacg ctcagctatt tggaatcgga atggcgtccg ctgcgaagaa
     4861 atctagccgc atggaatcag cagctgaccg gctacctgag ccggctcgaa ggtgagatag
     4921 ccgagttcga cgagcttagt aaggtttggc gagaaactct cgaatcggcg aatgccgcaa
     4981 acgtaccgcg ggaagtgctt attagaattc aggcggtcct cgatgagacc gaaaaggcgc
     5041 ggaaagagat cgagggttat cgcgggcgcg ccttgacctt acaaaatcga gtagctgcgc
     5101 aagacgggcg cattgccgat gccctgtcgt tgattgcaga aacacgcgag gaagcgctgg
     5161 accgtctttt taccccggac agcgcaccta tctggagcat cgacctacgt tcgtacaccg
     5221 cggaggtgct tcgagcccag agcagcaatt cggttgcgac gcagtggcaa gccctgagcg
     5281 cctatgcggc gcgccagaca ccgtcattcc tgcttcacgc gggaattttc atcggcatgg
     5341 ccgcgggact tttatggctg ggccgacgag tccagccgcg gctggaggat accgggattg
     5401 cgcgcacaac cattgtcttt gaaacgccag tcgcagccgg cttcctcgtg tcaatatttt
     5461 tcagccgttg gatctatccg caggcgccgc cgcgcttgtt ctgggcgttt ctcggagtta
     5521 tagctctgat ccccaccgca gtcatcttgc gcaagcttat cgcgcgtgac ctttatccag
     5581 ctctgtacgg tcttgccacg ttttatgttg tcgatcaggt gcgcgcgatt ttgatcgctg
     5641 aacaagtggt ggcgcgcctg ctcttgttcg gcgaaacgtt gggtttgatg gcgtttctgg
     5701 tctggctgct ccgttctatt gatcggctgc gcagtcagaa caccaccttc gatcgattga
     5761 gcaaaatcgt caacctagcg gcggttctgg ctttgataat ggccggcgcc ggtctcatcg
     5821 ctaacgcctt tggatattcg acgctggcta gccttctcgg caacacgctt ctgagcagcg
     5881 gttactttgc gctgattctt tatgccctcg tcgagttgat agatagcttc gtggtcatcg
     5941 cggtcagcct gcgaccgttc agaacgctcg gcatcattgc caggcatggc gcactgcttc
     6001 ggcgtcggat ttatcggatt ttacaatggg cggcggtgct cctgtggatt gttcatatgc
     6061 tcaatggcct gttgttgcgt caacggctgt ttagggccgt cagcgacttt gtgaacgaag
     6121 aactggttgt cggctcgctg cggatttcgc ttagcgacat agttgtcttt ggtgtcacgg
     6181 tttgggcagc cgttctggtt tcacgattcg tccgttttgt tctcgatgag gagatttttc
     6241 cgcgcgttca tctgacccgc ggtttgcctt acgccatttc cagcatggtt aactacgcaa
     6301 ttgtgctcgt tggatttttt gcagccgtgg ccgcattagg cgtcgatatg acgagagtga
     6361 caatccttgc cggagccttc agcgtcggtg tcgggatcgg cttgcagaac attttcaaca
     6421 acttcgtctc gggtctgata ttgttgtttg agcggccgat taaggtcggc gacatcgtgc
     6481 agatcgatga cgcttcgggc gtcgttcacc gtattggtat tcgtgcgagc atcatcggca
     6541 cgccgtccgg cgcccaggta atcgttccca acggcaaact gatatcggaa cgggtgatca
     6601 actggacgtt ttccaaccgc gggcgcagta ttgagctgca gttggctgtt gccccggtcg
     6661 cagatccgcg ccgagtcatt gccttactcg agcgcgccgc caacgaacat ccacacatca
     6721 gccgcgatcc tgctcccaag gcgtgggttg taaaactcgg tccggattcg cttggactgg
     6781 agctgcgcgc gtggaccgat cgcatacatg actggatgca agtgcgtagc gagcttgcga
     6841 tcgctgtcag cgataactta gcggccgaaa atatagccct gcgcgcagtt tagtgaccgt
     6901 tggcggttgc gccgtaaatt cacatcgctt tgctgccgta ggtgttggcc ttcaaaaagt
     6961 atctgctgga ttcagtggcg aattgacggt ccggctctgc taggccttct tggcgaatcc
     7021 ctcgacagcc tcgatcgtcg gtccattcag ctgaaaggcg cgatgtaccc gccattccaa
     7081 cacttgcatc tcgcgaatac cgcgaacgtg gtgcggatcc tttgctgcaa ggccctccgc
     7141 gtccttaagg ctggaggcca gcatgatgta aatgccgaaa cggccgtccg tggtagggcc
     7201 ggaaaaaagc aagcgtcccg ccgtgtgttg attgtccaac cattgccggt gctcttcgta
     7261 atggctctgc ttttctgctt ctttatcggg caacgatcgg gaaataacga tataccacat
     7321 gttaacctcc ggtttctcgg ttcaagggac ggttgcttaa attctcacat ccatgcaggc
     7381 ttgaagatga acaaaatcgt cggacagttc gaaggctcgg ctgaatcttc ggcaaatcaa
     7441 cattgcgcgc tgctcgaatg aaaattgatc cttgtgtatt atctccccgc ccgcgatatg
     7501 ctaactatct tggcagtaga gatgaattca tcatcgtgtg ccttgcctgc ggattgtagc
     7561 agtgtgtcac tcgatagatg taacaaagtt caatcggcaa agccaagaca tgctccattt
     7621 ctgaaacaac cggtactccg tgtcccgcgg gtaacgctat tgttttgacc aggcggcgct
     7681 cacagaaatg gtgaagatga aagatcatcc cagcagagta tcggcatcat atgccgataa
     7741 tgatggcata aaagttgcgc tttttacgcc ggcgctcgat gaatggaagg agacttgtat
     7801 gaaccgagta gaaattactg gcaaccgtaa ccacgacaaa tcactgggat cattgatctc
     7861 agggatcttc caagacgccc agacattaat aggcaaagaa atcgcagcag ccaaactaga
     7921 gctcaagcaa gagatgagca agggaatcaa ggctggcgtt tcgctcggaa tcggcggctt
     7981 tttactggtt gtcggattca tcttgctcac tcttatgatc gtgttcttaa tttcaaccta
     8041 cacgccgata ccgctttggg gcagccttgg catagttggg ttggtctatt gtgtcgcagg
     8101 cgctattgca ttgattattg gcaagcgcaa ggttacggag gtaaagccgg taccggaaga
     8161 gacgctacag cataccaagg aagatgtgcg gtatatcgcc gaacgcgcgt cgtcaggact
     8221 tgaaaaaggc gaaatcagaa aagctaccca gcattagcaa tggaatacga tccgacagga
     8281 aaccaggtcg ccgatcactg agaattcctt gctcctgtca cacccgactc atggccccat
     8341 gggttgtggc aactttatgc acgccggaga agaaaagtgt ccggcgtgca aagaccatat
     8401 cgattctgca aacgtgccat agaagcccga cggcattctc gccacatgta gcacctcaag
     8461 attcttcttg gtcgaaattt caagctccgg ttatcaatat tgccgaactg cggcggagct
     8521 aaatggcgca agcgcagcga tacgacgccg tggttgttgg ttccgggcca aatggactgg
     8581 ccgccgctat caccatggtt cgaagcggta gatccgtggc tgtgtacgaa gcggcagaaa
     8641 aggttggtgg cggaatgcgc tcggcagagt taaccttgcc tgggttcgtg catgacgtgt
     8701 gctcggcagt ttatccactg gcgattggct caccactgtt tcgttcgctg cctttggatg
     8761 agtatggact gaaatgggta cagccgccgg ccgcgctggc ccatcctctc gatgatggct
     8821 cggcggtctt acttgaaaga tcgttggacc acacggccgc cggcctcggt cgcgacggca
     8881 atgcgtatcg gcggttgatg cgtcctttgg tgaattcatg gcaggacctc gacagcgatc
     8941 tgctcgggcc actcagatgg ccgcgtaagc ccgtagcaat ggcgcgattc ggcctttcag
     9001 caatccgccc ggcgcggagc tttagcgaga gttggtttga agaacagccg gctagagcgc
     9061 tcttcgccgg gttatctgcg cattcgatgt tgcccttaga agcatggggt tcggctgctt
     9121 tcggcttggt gttggcgatc accggacatg cgttgggctg gccgcttgcg cgccgcgggt
     9181 cacagtcgct ggcggaggcg ctgggtttgt atctccgctc caaaggcggc gagatttaca
     9241 ccaacgcacc cgtgcgaacg ctggatgaac ttccttcctg tagggtcatt ctatgtgacg
     9301 tgacccccaa gcaactactg gcacttgccg gcgatcggct atcaccgagt tatcgccgcc
     9361 ggcttcagcg ttatcgttat ggcatgggtg ctttcaaaat cgattgggcg ctttcagcac
     9421 cggttccatg gaaggcgcac gcatgtgcgc gcgcggcaac ggttcatttg ggcggcagat
     9481 tgcaggagat agcagcttcg gagcgcgcgg cctggcgcgg cgagcacacg acagaaccct
     9541 ttgtcctggc ggtacagccg tccctattcg actcatcgcg ggcgcctgaa acaaagcaca
     9601 cgctatggac gtactgtcat gtgcccaacg ggtcgcattt tgatatggct gcacggattg
     9661 aagctcagat cgagcgtttc gcgccgggct ttggtgacac cgttcttgcc cgcagcgtgg
     9721 ctgggccggc tgaactggag cgacgcaact ctaacttgat cggtggcgac atcaacggcg
     9781 gcgcgccgac tttgagccaa ttgtttttac gtccgacact gaagctgtat tcgacttcag
     9841 cgcccggact ctacctctgc tcggcatcca ctccgccggg tgggggtgtc catggaatgt
     9901 gcggatattt tgccgctcgg caagcattaa gggattgttt ttagttcttt atcaaggagg
     9961 aacctgggtg aacggcgtgt gatcccggag gcattcggtg catcgttttg ccggcgcatt
    10021 tacccgctta tggcagcggc cgagaaaatg ggtgccgtga gtgcaggtac attgagccgt
    10081 ggtctctgca ttagtctcgt tatttttgtc ctcaatagtc atcgtctaac tggatccaga
    10141 gacatcctgg gcctaaggtt acatgtcgcg cgctctggcc gaatcatttg agatatccat
    10201 ttgacgcatt agaatgcggc gcagagcgtg gctcttcttg gttacggcgg atgaccctgt
    10261 cggcggtgcg ggtaagtacc gacggtgcca taaacgagtg actttgccat cctcgtttca
    10321 gagcattgcc ttcgccggag cgcgtgcttg gtaatgctcg tgaaataagt cccagaactt
    10381 ccgcggtaat gtttggaaat aaggaattgg ctagatgaag aagacgcgct tgaggagtga
    10441 taatcagttc ggctttgccg tagcggcacg cctcgacgat ttgccgtgct gctcgttgcg
    10501 acgctgtcga gaataagggt gacgcgttgg caatactgaa aagcgcgaac tctttctgat
    10561 gctgcccctt aaaaaaggca ttgatgtggg agccagtgcg catcaaaccg ggaaccaccg
    10621 tggttatgta aatcccatcc tttatcaact ccgcgcgcat tgcctcggaa aagccaacca
    10681 gagcaaattt actggcgaca tagggcgcca gatgaggaac ggcgaccttg ccgccgatgg
    10741 acgtaatgtt gacgatgcgg ccgctgcctt gccccttcat gtaggggagc acttccgccg
    10801 tcagattgag aggcgcccag aaatggactg ccaacgcatc ggcataatcg tcccggttca
    10861 tgttctcggc aggcccgact tggatgatgc cggcgttatt gaccagtaca tcgattgact
    10921 tgaactcatc aatgaccgat gtgacggcgc gccgcgcttc ctgagcttgg gttacatcac
    10981 actggcgcgt cgatatctca acgccgtatt gtttgagttc acgagcggct tctattaacc
    11041 gttcctggtc acgggccagc aagacgacgt tggcgcgttg cgctgcaaag cggcgggcca
    11101 attccaaacc caacccgcgg gatgcgccgg agatcagcac ggttttcccg gagaaatcga
    11161 ttctccggta acggtctaag gtgtaagaga aagcggctgc ggcgactccg gcggcggcaa
    11221 ttttcaccgg gagcgaaagc ttactatgcg aactgcttct catgatccct ccgtgatgat
    11281 cccgaaaaag ttccgatccg aggtgaaacg atgtgcatcg tggcttgcaa tcaccagctt
    11341 ggtgcgcctc agaaccaccg ttaaacatgt cgttttcagg cggaaaaaac gcaaagtata
    11401 tgccagaaac ccatggttct gaacacgtat cgcctatgac tctaggttgg acatcttgaa
    11461 aaaaggtgaa aaccagcgat gataactttc atggaaacga taaagatcga tgatccccgc
    11521 ctcgacgaga gttttgcttt catgttttca ttgagaaaag gcgatgccgt cttgctgaag
    11581 agtcaaaact cattgcgcgg caaagtcacc gacggcgttt atgtgggtga cttcccgaag
    11641 catgttgccg gagatatcca cccgcgggga gtaacgctgt acgagatttc acttgccgac
    11701 gatactatcc agatcctcga agaaaaagaa ctgcaaaaag tcgactaggc gtttctcgac
    11761 ttctagtttt cacttgtctc gaactgcggg cgcgtcgcag acactcgctc atcgaagtgt
    11821 ttaggtgact ggatttgtct tcatgtgcag gtgagcttcc attcacctcg ctaataatca
    11881 aagtgctggt cgatgagctc agtgtatcgg ttttgggtca aacggtttgt tcccgcctcg
    11941 tacgctgttc cagtcaaaat acacgcgccg ctcgatgatc aatgactttt taatggcacc
    12001 tgaattgctg aatattgtgt cgggacattc gttatgaagc agacgtccag atgcaaacga
    12061 gcaatcgtgc cgcttattct ttcgctgtcg tctgttggct gtgagagcat cgcccttatg
    12121 ccgcggccgg cgattgatga tcccgcagat gttagccggc gggatttgga tcggggggac
    12181 ttagatcgtc gtcctgaaaa ccgcgctgac ataccgcggt cgaagaccgc cgacgaagtg
    12241 accgggactg tgcaaaagat cgatcagaat cgccgtgaga tccagctccg cacgactgaa
    12301 gggcgtatga cggtcatcaa atatgatccg gcgacggtgg tttacaatcg agaacgcgag
    12361 atgccggtcg atgacctgcg ttacggtgac ctgatcttaa ttaagattgt aaagaataac
    12421 cgcggcgagc agtatgctga ccttattcgg cttgcgtcgt aagcgagggt gagggacacc
    12481 agtttaagat catgagtgga gatgctgcag atgatgatgg actgagcggc gaagccggtt
    12541 ctgcgttcat ggcgtaagat aaattctaca agccaaaact tttccctgtt ttaaattgcc
    12601 gttaatcgac tgttcggttg ggcgctgtgc actagcgtgc ctgcgacgca gccgatggag
    12661 tgtgtaatag aaccaatgct gccgtcatgt ccgttccgtc gcctaagtca catcatctgc
    12721 tgcaatcttc ttgttttgct ggcatttcta ttcgaatgtc gcttagccgc cggcaatggc
    12781 gtattcggat tggatcaagt cgctgagacc gcgcgtaaac tcgccgcaga acccttcaag
    12841 ccgccagccc ctgtaccaga ttttctcaaa caaataagtt acgacgactt tcgcgatatt
    12901 cggttcgata ccgatcaaag tttgtggaag gacgggggca acttccaggt gcagtttata
    12961 catcccggcc tttattacac ccatagtgtt gtgatcaaca caattgattt gcagggaatt
    13021 cgtaaagttg ctttttctcc gaaacaattc tcgtatggaa aaaataagtt tgccgataag
    13081 atcccgcctg aactcggctt tgccggattc cgcatcgcct atccgcttta taaaaagaac
    13141 gaatacaacc acgtcatcgt cttcgccggc gctagttatt ttcgcgctgt ggcgaagaag
    13201 cagggatttg gtctctcggc gcgcgggctg gccatcgaca cggccttgcc gagcggtgag
    13261 gaattcccga ctttcagaga attctggctc gaacggccca ccgctcaggc gcgcgctctc
    13321 aaaattttcg cgttgctcga tagccagagc ttaaccggtg cctacgagtt tatcgtgcga
    13381 ccgggagatc gcaccacggt cgatgtgaaa gcccggttgt tcgagagaaa aagggtgaaa
    13441 gagttaggag ttgcgccgct caccagcatg tttctgtatg gcgaggaaaa gccgcgaccc
    13501 gatacggact ggcggcctga ggtgcatgat tccgatggac tcatgatcgc gtccggtagc
    13561 ggcgaatgga tttggcggcc gctcattaat ccggtgaggc tgcaggtcag ctatttcgaa
    13621 ctcgacaatc tgcgcggctt cggtctgctc caaagggatc gcagttattc caactacgaa
    13681 gatctggaaa cgcgtcatga gatgcggccg aacgcttgga ttaccccttc aggtacctgg
    13741 cccaaagggc aaatcaagct agtggaaatt ccaagccaaa aagagaccaa cgacaacatc
    13801 gtcgcttatt ggattccgcg gagcatgccg gctgtgggtc aaccgctgga tctcgcgtat
    13861 cggattaact ttcaaaccga agaacccctc gatgcggtgt cgggccgcgt aaccgcaacc
    13921 cgtgtcggag ttggcgataa agaggatctc aagagaatta tcttagattt cgaaggtgct
    13981 aagctcaaaa gtttgcccga cacggctcca gtgaaagtgg tggtcacggt tggcgccgat
    14041 ggtcaattgg tccagcagag cacagtcaaa aatccgatta ccggcggctg gcgagccgcc
    14101 tttcaagtta aaccacctaa ggacaaaccg ctgcaggttc gcgcatttct aaaaaacgat
    14161 accgacactc tcaccgaaac ttggagttat ttattagagc catgaaagaa gatatttatc
    14221 agcccagttc ctcactcgcc cctggcggat caacctccga gattgctgca actgcaaatg
    14281 ggatttccga agtaatgacc acgaacggcg gtgccgttgc cgacgcgcca agcgggccag
    14341 gccctgcgac aatacgggag ctcaaccgac atacgcgcca atggggaaag gctgaacgcc
    14401 gacaaaggct tgtccaaagc gatctgaact cagttcgaga aaatatccat gcctgggcaa
    14461 tgcccgagat caagcgtctg tcgatgttgc cgaaccgaat ggaacgtaat ccgctggtga
    14521 cgctgtggcg cgctctgcgc ggacgaaccc gcaacaatga tatcgagaga actggccccg
    14581 cttcgacgaa agcagctctt gccggttggc gcggggccgc ggcatctcgg cgtagggtgc
    14641 tgattgcttt gatcataatc cagacagcca ttgccacctg gtctctcggt aacacctttc
    14701 cctacccatg gttgaaggcg tcggagatcg ccattctggt attatttgcc atcctgtttt
    14761 cgtggatttc gctggggttc tggagcgctg tggcggggtt ttgggtagtg tggaaacgta
    14821 aaaacgaatt taccggggct gatttacctg tcgatgatcg acaaccgtta cgctcgcgca
    14881 ccgcggtgct catgcccatc tgcaatgaag atgtgatccg tgtgtttgcc gggctggaag
    14941 cgagttatcg ctcgcttgcg gagacgtcgc agtttgcaag ttttgatttt ttcgtgctga
    15001 gcgacacggg tgatccggag aggcaagctg aagaggagat tgcgtgggcg caactttgcg
    15061 ccgccgtgca gggtttcggc agaatttttt atcgccacag gcgcaacaac gtaaggcgta
    15121 agagcggcaa catcgctgat ttcttgcgac gctggggacg agattacgat tacatggtcg
    15181 tcttcgatgc cgacagcatc atggccggcg aaactgttgt ccgactggct caaatgatgg
    15241 agcagtaccc tcaagcggga atcattcaaa cggcgccgac gacggttaac tgtgattcgc
    15301 tttttggacg gctgcagcag tttgcgagcc gggcgtatgg ccccgtgctg acggcggggc
    15361 aacggttctg gcaactcggt gacggctatt attggggcca taacgccatc atccgcaccg
    15421 cgccctttat caaacattgc ggcctggctc ggctgccggg ccgcgccccg ctgggaggtg
    15481 aaattctcag tcatgacttc gtcgaggccg cgctgatggg tcgggcgggt tgggaagttt
    15541 gggtcgttca tgatctaggt ggcagctatg aggaatcgcc gccaacgctc tctgatgaac
    15601 tcaagcgtga tcgccgctgg tgtcaaggta atctccagca tttgcgatta ctgttggccg
    15661 acgggattcg cgccgggcac cgatccattc tagcaatggg cgtgatggcc tatgcatcgg
    15721 ccttgctctg gtttgtgttt ttgatcctca acacggttga gcttgcggcg cagacggttc
    15781 tgcctctggt ctattttact agtgaaccca gcctatttcc gctatggcct cgctggcatc
    15841 ccgaatgggc ggtagccttg ctgacgacca cggccgtcct gctgttctta cctaagattt
    15901 tgagcctcct ggttattgtc aaaaatcgcc agacacatct gtttggcggc ttcctgccgt
    15961 tgtgtgtgag tatctgtctt gaaattattg tgtcgacttt gcttgcgccg atccgaatgt
    16021 ggtttcacag ccagttcgtt gtcgtgactc tcatgggccg tcagataaaa tggggaccgc
    16081 aatttcgcac cgataatgaa acccgctggc gcgacgcaac ccgattgcac ggtatagcaa
    16141 cgttattggc cttcggttgg gtattcggca tgttctggtt gaatccggca gtttcggtct
    16201 ggctgctacc agttggcatc tcgctgctgt tggccgtacc attatcagtt ttttcgagtc
    16261 gtctttcctt gggacggctc atgcgtcagt ggagattgtt cctgattccg gaggaaattc
    16321 gttcgccgca agttatcaat tatcttcacg catccctaat taaacggcga gaaaaccgac
    16381 agagggatgg ctttgttcag gcagtagttg acccctatgc caatgccgtc cacctaggcc
    16441 ttttgcgcgg caaaaaaccc cagacaagcg aagctatcga tcgcaaccga aacctgcgtg
    16501 aactcgctct gaggaacggt ccggcgatcc tgagccgtgc cgacaaaacg cggctgcttc
    16561 gagactcaga aaccatggcg cttctccacc ggcaagtgtg gcgtgttcgt gatactaatc
    16621 tggcaaatca gtggggattg agcgagctcc agtgaattcg tcaagctgag cttcatcatc
    16681 aattataaag atgatgattg attaatcttg ccaggctgaa tcagtcagcg ctggaaagtg
    16741 ccgctccgct agcgacgacg tgaacggccg caaaaaaaga ccgttgcggg ttcacccatc
    16801 atcaacggtt gatcctctgc tatgactcac tgtcccatac tactgatcgg gaccagctgc
    16861 gtctaatgat caggtgcatc ttagagcgcg atccggatta tggcgacgag cgcaaccatg
    16921 attttcacgg acatctcgca ggcaaaacct ggcgaatttt tctaacctgc tggcttttga
    16981 tatgatacaa agcggtgggt tgcccgacag gttaggcttg ttagtcta
//
LOCUS       DASUVS010000426        12627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_844222, whole genome shotgun sequence.
ACCESSION   DASUVS010000426 DASUVS010000000
VERSION     DASUVS010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12627
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844222"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..407
                     /locus_tag="VF182_22440"
     CDS             183..407
                     /locus_tag="VF182_22440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439894.1"
                     /translation="MTAANYQPVINAINCSWGAQLMSNLPHHCKRLRMRFFFPAVSEL
                     SGSFRERQRLEKNFMDERFERSEAVERFEQ"
     gene            complement(423..1193)
                     /locus_tag="VF182_22445"
     CDS             complement(423..1193)
                     /locus_tag="VF182_22445"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6439895.1"
                     /translation="MLEAFQAGRVARVTIFYAGAILLWEMLVKLLQVPDWLLPAPSQI
                     VDVIIDKNAVMLSHTWVTLYETVLGFALALVLGVVCAIGIVHFPFLRDTLYPGLIMFN
                     SFPKVAIAPLFVVWIGIGIESKIANAFLTAVFPIVLNTIMGLTNMDPELLELVRSMSK
                     SRWVWFWKIRLPHSLPYLFAGCKIAISLAVIGAIVGEFISGRSGLGYLVLSANNYFNT
                     PMAFTALLYLVVMSLALYGLVILAEKLILPWSRRETAG"
     gene            complement(1186..1959)
                     /locus_tag="VF182_22450"
     CDS             complement(1186..1959)
                     /locus_tag="VF182_22450"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6439896.1"
                     /translation="MPESIRVERLSKVYQSRDGTEVQALRDVCFTVGRGEFVSIVGQS
                     GCGKSSLLKILAGLLFKTSGVISIEDRELDGPRSDIGLMFQKPLLLEWRRVLDNVLLP
                     VEIYRLDRKEYQEKARRLLRAAGLADFLTKYPFELSGGMQQRVALCRALVAEPSLLLM
                     DEPFGALDTLTRQKMGLELLRLWEEWKSTVLFVTHDVDEAVMLSDRVLVMSSRPGTVL
                     DSFTVDLARPRQIFIKDTAEFIRISSRIRRCLWEEAADA"
     gene            complement(1961..3004)
                     /locus_tag="VF182_22455"
     CDS             complement(1961..3004)
                     /locus_tag="VF182_22455"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439897.1"
                     /translation="MKKSLCAMLATIFAAAVAAGFPPHSEAQSKKVVIQLAWVVGGLH
                     AGFFVAKEKGFYSSRGLDVSINRGFGSGDTAKVVATGTAQFGEVNVPTAIISRGKELP
                     LTVTGVIIGKAPESILSFADKGIRKPKDVEGKTFAEASGAAIMVTWPAFAKLAGIDIS
                     KATHIPVEAAAKPAVFFSGQVDWVPGFRPGFDEPLIIRARKEGKKLHFIRWEDFGWKV
                     YGNGLVTNNDLLKREPKTVANFVSATMEGYAYAIAHPDEALDITLKANPELERESARL
                     SLLFAIDAMMTKASQEKGLGYMEDDRMAFQIKLMSDLMKISPPKDVYTNQFIQKKSVT
                     VPPALAAELSKLP"
     gene            complement(3156..4418)
                     /locus_tag="VF182_22460"
     CDS             complement(3156..4418)
                     /locus_tag="VF182_22460"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6439898.1"
                     /translation="MEKTLWFPLNRIVRLRAFQALGHREYRLLWYSQVFASLGVWMDQ
                     VTRGWLIYELTDSALQLGMVRGIQAMPFLFLSPVAGSVADRYPRKFLIVTAQFANGFI
                     FTATAALILFGLIRPWHVYLTAVLMACVQVFLQPARAAMISDTVPSENLTNAIGLNAV
                     VFNMARSTGPALAGVLISIFDTGVAYGAQGLFFLLATVWTIQMRSGRRAAAARRSHGG
                     GNESFARSIVSGWQFSWRKDEVRSSLLIVIVASLFMVPFSTLLPIFARDILQVGAQGQ
                     GLLLTSMGIGALGSALIIATFGDRMPRGIMMLAGVTLYGVLVVLFSASPWFSLSMFLM
                     GVIGLCHVSSHALVQTVIQSYSPPEFRGRAIAMFHMNQILLLLGGLVIGALSEAIGAP
                     WAAAALSIAGTLCMVALYVLDPRAREIR"
     gene            4717..5745
                     /locus_tag="VF182_22465"
     CDS             4717..5745
                     /locus_tag="VF182_22465"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6439899.1"
                     /translation="MKRLLCALLFLPALVTNVQAQAPFYQGKTIRIIVGNLAGDAYDL
                     WARIFAQHMGKYIPGNPTFIVQNMTGAGGIIAANYVYSVAKPDGLTLGTFGPSMYFDQ
                     LAKRPEVKFDWAKFSWIGTPEQTEFILIMRTDTPYQSIEDVRKAAQPPKCGATGTSTS
                     GYYMPKFLDEGLGIKFNIVSGYPGGGEIDLGIERGELQCRNLTISTYFAREPFLTWHK
                     KGFVRPILQTGKKRDRLVPDVPSVYELMDQLNTPEETRRVATVLLAPAIFGRPMVVTP
                     GVPADRLKILRDAYEQSLKDPALLEELKKRRWSVDAVGGEELAKLAKEVVSQPPDVVE
                     QMMKLLGN"
     gene            complement(5967..7355)
                     /locus_tag="VF182_22470"
     CDS             complement(5967..7355)
                     /locus_tag="VF182_22470"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX6439900.1"
                     /translation="MTFHRGILPIALALGIYVAAALGSVGFSRLASAHEFTMESLINA
                     FVKIESHEAHLIARVPLEILKSVKFPVSGREIDLAAADSVIQQALTQLDREMTILEAG
                     RPLVASRALGRLTLPSDRSFESYPDAVAHIARPIAAGTSIFVDQGFLDVHFSYQISSP
                     NSRFMIQTAVAPELKNYLKFAIRYLPLSDESRAMMITSRSGRVALNPTWYQAAAGFVT
                     LGIVHILSGIDHLLFLLCLLIPVRGIGEILRIVTAFTIGHSITLVGSAYDLAPAGAWF
                     PPLVETLIAASVLYMAIENILSVNLRTRWLIAAIFGLVHGFGFSYGLKENLQFAGKHL
                     LVSLFSFNIGIEIGQIFVLIIMLPLLALLLRRVLVGPAGMIILSAIVAHTAWHWMIER
                     GDVLWQVEWPALDEAGITILARWLAGLLILGGGVRFLAMRSHVRRRFHPSLNGGELPD
                     SRGQRREASAGM"
     gene            complement(7422..8666)
                     /locus_tag="VF182_22475"
     CDS             complement(7422..8666)
                     /locus_tag="VF182_22475"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEX6439901.1"
                     /translation="MRIWRWVYVGTLLVTLVVGLGVANPLRDMDVKDSRVPQYVVDPF
                     WPKPLPNDPETGKPWVTGEVAGSCVDSRDHIFTVNRGYNNGLVAPETVVAVPSPPVVE
                     YDPHGNVVNAWGEPAILPNGVHGCFVDYQDNVWIAGNGDGIVQKYSRKGELLLQIGTK
                     GVCDWPANNNACGNSGADPNANQSRTLLNQPSDVAVDPANGDIYIADGYGNHRVVVFD
                     KNGTYLRQWGSVGDGPGQFGAADGGHPHCVVLGNDGLVYTCDRGQDRLEVFDKSGNLV
                     KIIPVIPGTGTPGLGTAGSAWDVEFSPDRRQELMFNADGGNEVVWTFNRLAALAGAAL
                     TATPGQGILAGFGRPGHNAGEFTFLHTVAADSKGNLYIAETVGGRRIQKFVPRRGKGN
                     DLHVFTPAGNPDQLLPHYDPVK"
     gene            complement(9166..10371)
                     /locus_tag="VF182_22480"
     CDS             complement(9166..10371)
                     /locus_tag="VF182_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019854126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX6439902.1"
                     /translation="MRRSTDRILTTHVGSLPRPADLRELWSKQTSGSAEDALQARLRS
                     AVAEVVLAQKAAAIDIPNDGEFGKPMRAASDRGAWGNYIFNRVSGFVPTPPEAVAPDT
                     AAPGAPMRIVGVRWEQREFAEFYADTGLGAPSTAASRPMCAAPIAYTGHEALRRDLAN
                     LRAAMDQAGVQDAFVASIAPGSLEMFCRGQNSYYPTAEAFIEAIAAAMREEYRAIIEA
                     GFILQLDDPGLPGAWDMLDPRPIINEYRRYAMLRVEALNRALEGVPEDRVRYHICWGS
                     WHGPHTTDMPLRDIADVMLKVKAQAYLTEAGNVRHEHEYKVWRDIKLPAGKILIPGVV
                     SHATPVVEHPELVADRILAFANAVGRENIIAGTDCGLGGRVHPQIAWAKLRALSEGAA
                     LASKKLWET"
     gene            10649..10951
                     /locus_tag="VF182_22485"
     CDS             10649..10951
                     /locus_tag="VF182_22485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439903.1"
                     /translation="MADRKHGHSLRSHCSGCLVYDLVDWTEERIRPDGRRDRWVAPTV
                     GFEFLAGHYVFGNSWEILIADYNVFRGRIWILVLAANIFAPLYRRFCRSRRYSGKE"
     gene            complement(11063..12145)
                     /locus_tag="VF182_22490"
     CDS             complement(11063..12145)
                     /locus_tag="VF182_22490"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6439904.1"
                     /translation="MAIIERTYGKETNAAINELLISSDSHVIEPAGLWQKQLPKAFQD
                     RAPDYGGQRPNDQPGGAQDKNKRVSEMAADGVSAEVLYPTHGLKCLSLDDHELEAACC
                     RVYNDWLIDYCSAAPERMIGIAMLSMYDSDGAVKELERCRKAGMRGSVIWQVPPPELP
                     FSSSHYDKFWAASQDLEMPVNLHILSGHGYSRNRSFGLGDTATGMTAEHNSVNSKLAQ
                     SVDSLYDLIYSGVLERFPKLKVVLVENELGWIPFVLEQWDYYFKRFGAKREGLLIKKR
                     PSEYFHNQIYTTFFNDAVGGHILSWWGQDNCMWSNDFPHGNTTWPHSRKVVARDLGDL
                     PSGVRAKLVRENVARLYNIPVPSPVQ"
BASE COUNT         2829 a         3586 c         3586 g         2626 t
ORIGIN      
        1 cgcttgatgc tgtgccttgg tggttgctca gaccaccaac gtaccgctgg ataggtgtac
       61 cggatgcgcc ctgaacatat tacgcagaaa acctattttg ttttctgacg gactaccgcg
      121 aacggaaaga actgtctcaa actagaaacg ctggcacatc cacacactgg tagttgttgg
      181 gcatgacggc agccaattac caacctgtca ttaatgcaat caactgtagt tggggagcgc
      241 agttgatgag caatctgccg caccactgca agcggttgag aatgcggttc ttttttcctg
      301 ccgtgagcga attgtccggc tcgttccgcg agcgtcaacg gttggaaaag aatttcatgg
      361 atgaacgttt tgaacgaagc gaagcggttg aacgttttga acaatagagt cattgacccg
      421 attcacccgg cagtttcccg gcgcgaccag ggaaggatga gtttctccgc caaaatcacc
      481 aaaccgtaaa gcgccagcga catcaccacc aaatagagaa gagcggtaaa cgccatcggc
      541 gtgttgaaat agttgttggc ggagagaacc agatagccga gccccgacct tcccgagatg
      601 aactctccga caatcgctcc aatgacggct aaagagattg ctatcttgca tccggcgaaa
      661 agataaggaa gcgagtgggg caggcgaatt ttccagaacc acacccaacg actcttggac
      721 atggaacgaa ccagctcgag caattccgga tccatgttgg ttaaccccat gatggtatta
      781 agcacgatcg gaaacaccgc ggtgagaaag gcgttggcga tcttcgattc gatgccgatg
      841 ccgatccata cgacaaacag aggcgcgatc gcgaccttgg ggaatgagtt aaacatgatc
      901 aaacccggat agagcgtgtc acgcaggaac ggaaagtgga cgatcccgat tgcgcaaaca
      961 acgccgagca ccaaagcgag agcaaagcct agcaccgtct cgtacaaggt tacccatgtg
     1021 tgcgacagca tcacggcgtt cttgtcgata ataacgtcga cgatctgaga aggcgcgggc
     1081 agtagccagt ccggcacctg gagcagtttg accagcattt cccaaaggag gatcgcgccg
     1141 gcatagaaaa tagttacgcg cgcgactctt ccggcttgaa aggcctcaag catcggcggc
     1201 ttcctcccac aggcagcgcc ggatgcggct gctgatccgg atgaactcgg cagtgtcttt
     1261 gatgaagatc tgacgcggac gcgcgagatc gaccgtaaac gagtccagga ctgttccggg
     1321 acgggaagac atcaccagta cccggtcgga gagcatgacg gcttcgtcaa cgtcgtgggt
     1381 cacgaacaaa accgtgcttt tccattcttc ccacaaccgg agtagctcaa gacccatttt
     1441 ttgccgggtt agtgtgtcga gcgcgccgaa aggttcgtcc atcaaaagga gcgaaggctc
     1501 ggccacgagc gcgcggcaga gggcaacccg ttgctgcatg ccgccggaaa gctcgaacgg
     1561 atacttggtg agaaaatccg ctagaccggc ggcgcggaga agcctgcgcg ctttctcttg
     1621 gtattctttc cgatcgagcc ggtagatctc caccggcagc aacacgttat ccagcacgcg
     1681 gcgccattcc aacagcagcg gcttctggaa catgagaccg atatcgctgc gaggtccatc
     1741 cagctcgcga tcttcgatag agatcacacc gctggtcttg aaaagaaggc ccgcaagaat
     1801 ttttagcaag ctcgacttgc cgcagccgct ttgaccgacg atcgaaacaa attctccgcg
     1861 ccctacggtg aagcagacgt cacgaagcgc ctggacttcg gtcccgtccc ttgactggta
     1921 taccttggaa agccgttcga cgcggatgga ctccggcatg ctagggtaat ttgctcagtt
     1981 ctgctgccag cgcgggaggc acggtaaccg acttcttctg aatgaattga ttggtgtaca
     2041 cgtcctttgg cgggctgatt ttcatgagat cggacatgag cttgatctga aaggccatcc
     2101 ggtcgtcttc catgtagccc aatccttttt cctgcgacgc tttcgtcatc atcgcgtcaa
     2161 tggcgaataa cagcgaaagc cgggcggatt cccgttccag ttcagggttg gccttgagcg
     2221 tgatatcgag cgcctcatcg gggtgcgcaa tggcgtaagc atagccttcc atagtagcgc
     2281 tgacaaaatt agcgaccgtt ttcggttcgc gcttgagcag gtcgttgttg gtaacgagac
     2341 cgttgccgta aaccttccag ccaaagtcct cccatcgaat aaaatgcagc ttcttccctt
     2401 cctttcgagc gcggatgatc aatggttcat caaagccagg acgaaacccg ggaacccaat
     2461 cgacctggcc gctgaaaaac accgcgggct tggcagcggc ctcgactgga atatgggtcg
     2521 ctttgctgat gtcgattccc gccagcttgg cgaaggcggg ccaggtcacc ataatggccg
     2581 ctccggatgc ctccgcgaaa gtcttgccct cgacatcttt gggcttgcga attcctttgt
     2641 ctgcaaatga taggatgctc tccggagcct tgccgatgat gacaccggta acggttaagg
     2701 gcagttcttt gccacgcgaa atgattgccg tcggtacatt cacttcaccg aactgtgcgg
     2761 ttccggtggc gactaccttg gcggtgtcgc cgctgccgaa gccgcggttg atgctcacgt
     2821 ccaagcctct ggaggaatag aagccttttt ctttggcgac gaaaaagccc gcatgcagcc
     2881 cgccgacgac ccaggccagc tgaatcacga ctttcttgct ttgcgcttca gagtgaggcg
     2941 gaaaacccgc tgctacggcg gctgcaaaaa tcgtagccag cattgcgcat aaagatttct
     3001 tcacgacgac ctcctcctct tcgttttgta ttctcctaaa atgagaaact ttctttgacg
     3061 taagctatca gcctgattcc attcaagtca aagatcatgg tgcacccttg atcaggccgg
     3121 ctacactgaa agagacttta atcttctgac cgatatcaac ggatttcccg cgccctggga
     3181 tccaagacgt agagcgcaac catgcagaga gtgccggcaa tactcagcgc cgccgccgcc
     3241 cagggcgcgc cgatggcctc cgacagcgcc ccaatcacca ggccgccgag cagcagcagg
     3301 atttgattca tatggaacat ggcgattgcc cggccgcgaa actcgggcgg tgagtagctt
     3361 tgaatgacgg tttgcaccag ggcatgggag ctgacatggc acaggccgat gactcccatc
     3421 aggaacatcg atagcgagaa ccacggcgaa gccgaaaaca gcaccacgag cacgccgtaa
     3481 agcgtgacgc cggcgagcat catgatgccg cgcggcatgc gatcgccgaa ggtcgcgata
     3541 atcaatgcgc tgccgagcgc tccgatgccc atcgatgtca gaagcagccc ctgtccttgc
     3601 gcgccgactt ggagaatgtc tcgcgcaaag atcggtaata acgtggaaaa cggcaccatg
     3661 aacagcgagg cgacgataac gatcaggaga ctgctccgga cttcgtcttt ccgccaggaa
     3721 aattgccatc cggacacgat gctgcgcgcg aaggactcgt tgccgccgcc gtggcttcgt
     3781 cgtgccgcgg cagcgcgtcg tcccgatctc atctgtatgg tccagacggt cgctagaaga
     3841 aaaaacaatc cttgggcgcc gtacgccacg ccggtgtcga aaatggagat cagcacgccg
     3901 gccaaggccg gtccggtgct gcgcgccatg ttgaaaacca cagcattgag accgatagcg
     3961 ttggtcagat tctccgaggg gactgtgtcg gagatcatag cggcgcgggc gggctgaaga
     4021 aagacctgca cgcaagccat cagaacggca gtcagataca catgccacgg ccgaattagt
     4081 ccgaagagaa tcagcgctgc agtagccgtg aaaatgaagc cgttggcaaa ctgcgccgtg
     4141 acgatcagaa actttcgggg ataacggtcg gcgacgctgc cggccacggg tgataggaaa
     4201 agaaatggca tcgcctgaat gccacgcacc attcccagtt gcagcgccga atcggttaat
     4261 tcgtagatca accatccccg ggtcacctga tccatccaca cgccgaggga ggcaaaaacc
     4321 tggctgtacc agagcagacg gtactcccgg tggccgagcg cctggaaggc acgcaaacga
     4381 acaatccgat taagcgggaa ccaaagcgtc ttttccatgt tggtctgcga gtgtttcgga
     4441 ccgcggcgca aggggcgtcg cgagtggctc gacggcgatt gcagaaagaa cttcggattg
     4501 ccgaacgaga cgagcagtcc cgctgatttg aagcccgggc gttaaggatc gctactcttc
     4561 tatctcacag ccggaaaaag ttccagccga taactacttt gctccgcgca ttccaatttc
     4621 cttgacacat tcgttgcgcg cgccttatga tccgcggcgt ttcgcataat agagagattg
     4681 gttctcacgc ctatcagaga caatccggag ggtgcgatga aacgcttgct ctgcgcgctg
     4741 ttatttttgc cggccttggt aactaacgtt caagctcagg cgccgttcta tcaaggaaaa
     4801 actatccgca tcatcgtcgg taatctcgcc ggagatgcct acgatctgtg ggcgagaatc
     4861 ttcgctcagc acatgggcaa gtacatcccg ggaaatccca cgttcattgt ccagaacatg
     4921 accggcgccg gcggaatcat cgccgccaat tacgtttact ccgttgccaa gccggacggg
     4981 cttacactgg gcacattcgg accctcgatg tacttcgacc agctggccaa gcgtcccgaa
     5041 gtgaagttcg attgggccaa attttcgtgg atcggcacgc cggagcaaac ggaattcatc
     5101 ctgatcatga gaaccgatac gccgtaccaa tcgattgaag atgtgcgcaa ggccgcgcag
     5161 ccgcccaagt gcggcgctac cggcaccagc acttcgggtt attacatgcc gaaattttta
     5221 gacgaagggc tcgggatcaa gttcaacatc gtgagcggct atcccggtgg cggcgaaatc
     5281 gatctcggca tcgagcgtgg cgagctccaa tgccgcaatc tgacaatcag cacgtacttc
     5341 gcgcgcgagc cgtttctaac ctggcacaag aaggggtttg tccggccaat cctgcagacg
     5401 ggcaagaagc gagatcgtct ggttcctgat gttccgtctg tttatgaatt gatggatcaa
     5461 ctcaatacac cggaggagac ccgtagagtg gctacggtgc tgctcgcgcc cgcgattttc
     5521 gggcgaccga tggtggtaac accgggcgtt ccggccgatc ggctgaaaat cttgcgcgat
     5581 gcctatgaac agtcgctcaa ggatcccgcg ctgctcgaag aattgaagaa acggcgatgg
     5641 tcggtggatg ccgtcggcgg tgaggagttg gccaagctcg ccaaagaagt ggtttcgcaa
     5701 ccgcccgacg tcgtcgagca gatgatgaag ttgttgggca attagcccta atccgacaat
     5761 agttggcact ttcagcagcg agcgaggacg tagagaccgg aagccgtggg caccgaacgc
     5821 gcagttgact ctaaggcctc gacatacgaa acatcaccct ttggggaatg cggagcggcc
     5881 ttcaaggcac tggccccatt gcccacgact gtagggctcg ctggcctcgc ggggttagaa
     5941 taagtcggat taggggttag ctccgatcac atgccggccg aggcttcgcg ccgttgcccc
     6001 cgcgagtccg gcaactcgcc gccattaagg gatggatgga agcgtcggcg gacgtgcgag
     6061 cgcattgcca aaaaacggac tccgccccct aagattagaa gtcccgccaa ccaacgagcg
     6121 aggatggtga tgccggcttc gtcaagcgct ggccactcga cttgccagag cacatcacct
     6181 ctctcgatca tccagtgcca ggcagtgtga gccacgattg ccgaaaggat gatcatgccg
     6241 gcgggcccga cgagcacgcg ccgcagaaga agtgcaagca ggggtagcat gatgatgagg
     6301 acgaaaattt gtccgatctc gatcccgata ttgaacgaga aaagcgagac cagaagatgt
     6361 ttcccggcaa attgtaagtt ctctttcaag ccataggaga acccgaatcc gtgtacgagg
     6421 ccaaagattg ccgcgatcaa ccaccgcgtg cgaaggttga cgctcagaat attctcaatc
     6481 gccatataca gaaccgatgc cgcgatgagc gtctcgacca acggtggaaa ccacgcgccc
     6541 gcgggagcca agtcgtaggc tgagccgacc agcgtaatcg agtgaccgat tgtgaatgca
     6601 gtgacaatgc gcaggatctc gccaatgcct cgcaccggga tgagaaggca tagcaggaaa
     6661 agcaggtgat caatgccgct tagaatgtgc acgatcccga gcgtcacgaa tccggcggca
     6721 gcctgatacc acgtcgggtt aagcgcaacc cggcccgaac ggctggtaat catcatcgcg
     6781 cggctctcat ccgaaagcgg cagatatcgg atcgcaaact tgagatagtt cttcagctcc
     6841 ggcgcgactg ctgtttgaat catgaaacgc gaatttggcg aactgatctg atacgagaag
     6901 tggacgtcga ggaatccctg gtcgacaaag atgctcgtgc cggctgctat cggtcgtgcg
     6961 atgtgggcga ccgcgtccgg atagctctcg aatgaacggt cggatggcaa cgtgagacga
     7021 cccaaagcac gagacgccac cagtggccgg ccggcttcca agatcgtcat ctcgcggtcg
     7081 agctgcgtca gagcctgctg gattacggag tccgcagcgg cgaggtcgat ttcgcgacca
     7141 ctcaccggga atttgaccga cttgaggatc tcgagcggta cccgtgcaat tagatgcgcc
     7201 tcgtgggact caatcttgac aaacgcattg atcaggctct ccatcgtgaa ctcgtgcgcg
     7261 cttgccagcc gcgaaaaccc aaccgaccct agcgccgcgg caacataaat gcccagcgca
     7321 agtgcgatag gcagaatccc acggtgaaat gtcatcacga cgtcctccag gtggaggcct
     7381 gccggaagta tcatcctatc cggcaggcga ttgggatgcg attacttcac gggatcgtaa
     7441 tgaggcaaca gctggtccgg attgccggct ggagtgaaga catggaggtc gtttcctttg
     7501 ccgcgccgtg gcacgaactt ttggatccta cggccgccga ccgtctcggc gatgtacagg
     7561 ttgcctttcg aatccgccgc gaccgtatgc agaaatgtga actctcccgc attgtgaccg
     7621 gggcgcccga agccggcgag gattccctga ccaggcgtcg cagtgagagc ggcgcctgcg
     7681 agagcagcca ggcggttgaa ggtccacacg acttcgttgc cgccgtccgc gttaaacatc
     7741 aattcctgcc ttctgtcggg ggaaaattcg acgtcccacg ccgagccggc tgttcctagt
     7801 ccaggggtgc cggttccagg gatcacggga atgatcttta ccaggttacc tgacttgtcg
     7861 aacacctcga ggcgatcctg gccacgatcg caggtataca cgaggccgtc gttgccgaga
     7921 acgacgcaat gcggatggcc gccgtccgca gcgccgaact ggcccggacc gtcgcccacg
     7981 ctgccccact ggcgcaagta ggtcccgttc ttgtcgaaga ccaccacgcg atggttgcca
     8041 tagccgtcgg cgatgtagat gtcaccgttg gcgggatcca cggcgacgtc tgatggttgg
     8101 ttgaggagtg tcctgctctg atttgcgttc gggtcagctc ccgaattgcc gcaagcgttg
     8161 ttattcgctg gccagtcgca gactcccttc gtgccgatct gcagcagcag ttcgcccttg
     8221 cgcgagtact tctggacgat gccatcgccg ttgccggcaa tccagacatt gtcctgataa
     8281 tcgacgaagc agccgtggac gccgttcggc aggattgccg gctcacccca tgcgttcacc
     8341 acgttgccgt ggggatcata ctcgaccacc ggcggtgacg ggaccgcgac aaccgtctct
     8401 ggagcgacca aaccattgtt gtaaccacgg ttgacggtga agatgtggtc gcgcgagtcg
     8461 acgcaacttc cagcgacttc gcccgtcacc cagggcttac cggtttccgg atcattgggc
     8521 agcggtttcg gccagaacgg atctacaacg tattgaggaa ctcgggaatc ctttacgtcc
     8581 atatcgcgca atggattcgc gacgccgagc ccaacaacaa gagtgaccaa gagagtgccg
     8641 acatagaccc atcgccaaat tctcatagcg acctcctttg cgggtttatg tcaatgacag
     8701 cattgcttta gagcccgacg cgaaactgaa aacccgctcc ctcagccgcg tgcaagcccg
     8761 ctatcgagaa ttcgatagcg aatccgacaa aggaaagcag tcctctcgtc ctaacttggt
     8821 gtggaagtta tccagtatct aatgcaacag cccttggcca atattttttt cttataaccc
     8881 tcattagatt gtcctatgac aacggctttc agatttcgct atggggcgaa gacacgaacg
     8941 ggatgaccga gctgcgacag ccggcccgag ttcgctctga actaattgaa cgacggagtt
     9001 tccgagagaa tgatttgatg aagttcgtcg tcgatggtca gcgtacgcca gaagttttaa
     9061 ttcgtcttgc agtcttgcac gccaattcat tccgcgcgaa tttatcaggc cggcatttac
     9121 gactcccgcc gccacagttg acaagagcgc ttgtcgatga agtcctcaag tctcccacag
     9181 cttcttgctc gccagcgcag cgccttcgga tagcgctcgc agctttgccc acgcgatctg
     9241 cggatgcacc cgcccgccga gcccgcagtc tgttccggcg atgatattct cccggccaac
     9301 ggcattggca aaggcgagaa tccgatctgc caccagctca ggatgctcga ctacgggcgt
     9361 tgcatggctg accacgcctg gaatgaggat cttcccggcc ggtaatttga tgtctcgcca
     9421 gactttgtat tcatgctcgt ggcgcacgtt gccggcttcg gtcaagtacg cttgcgcctt
     9481 gaccttcagc atcacatcgg cgatatcccg taagggcatg tccgtggtat gagggccgtg
     9541 ccagctgccc cagcaaatgt gatagcgcac tcgatcctcc ggaacacctt ccaatgcgcg
     9601 gttcaaggcc tcaacccgca acatcgcata gcgcctgtac tcgttgatga tcggtcgcgg
     9661 gtccaacata tcccaagcgc caggtaaccc gggatcatcg agttgcaaga taaacccggc
     9721 ctctatgatc gcgcgatact cttcgcgcat agcggcggcg atggcttcga tgaatgcctc
     9781 agctgtcggg tagtagctgt tctgaccacg acaaaacatc tcgaggctgc cgggagcgat
     9841 ggacgcgacg aatgcatctt gaacgcctgc ttggtccatc gccgctcgta aattcgcaag
     9901 atcacggcgt agcgcttcat gtccggtata ggcaattggc gcggcgcaca tcggccggct
     9961 cgcagcagtg cttggcgctc ccagtcctgt gtcggcatag aactcggcaa actcccgctg
    10021 ctcccagcgc actccgacga tgcgcattgg cgcccccggc gccgccgtat ccggcgcaac
    10081 cgcttcgggc ggggtcggta cgaacccaga gacgcggtta aagatgtaat tgccccaggc
    10141 gccgcgatcc gatgccgcgc gcatcggctt gccgaactca ccatcgttgg gaatatcgat
    10201 tgccgccgcc ttctgagcga gcaccacttc cgccacagcg gatcgcaagc gcgcctgcag
    10261 cgcgtcttcc gccgagccgc tggtttgctt cgaccacagt tcgcggagat cggcgggccg
    10321 cggaagactg ccgacgtgcg tggtcagaat tcgatccgtg cttcgtcgca tttaatacct
    10381 ccaattttaa tcctaacggt cagcctgggt attcagttat cgctaaatac gattttatcg
    10441 caagcacccg atcgcctatg tctcgattgc gatgaggcgg cgaactccct acgcgaccgg
    10501 tacagtaaag acctggatcc aacaagcacg tgaaagaatt ctatgttctc cagggctctt
    10561 gccatctggc ttttgctgct ggtttgccgc tgtgttgaat ggcgcgattc gcaaatcact
    10621 aattacaccg cggtttgggt gagcagggat ggcagatcgt aagcacggcc attctctgcg
    10681 cagccattgt tctggttgcc tggtttatga tctcgtcgat tggaccgaag aacggattcg
    10741 acccgatggg cgtagggata ggtgggtggc tccaaccgtc ggttttgagt ttcttgccgg
    10801 ccactatgtt ttcggcaaca gctgggaaat actgatcgct gattacaacg tgttccgcgg
    10861 ccgcatctgg atcttggtgc ttgcggctaa catatttgcg ccgctctatc ggcgtttttg
    10921 cagaagcagg cggtatagtg ggaaagagta aacgaaaccc ggactccgat aatcacctgt
    10981 ctttgcaatc cgaggagcgc gacccgaccg gcgaccgcac tcctcgctca acggatgcgc
    11041 aacgtactgt cgttatcggc gattactgga ccggacttgg aacaggaata ttgtagagcc
    11101 gcgcgacgtt ttcgcgcact agtttggcgc gcactccgga gggcagatcg cctaaatccc
    11161 tagctacgac cttgcgtgaa tggggccagg tagtgttgcc gtgcggaaaa tcgttggacc
    11221 acatgcagtt gtcttgaccc caccacgaga gaatatgacc gccgacggcg tcattgaaga
    11281 aagtcgtgta gatctggttg tggaagtatt cgctgggacg ttttttgatg agcaacccct
    11341 ctcgcttggc gccaaaacgt ttgaagtagt agtcccattg ctccaggaca aacggaatcc
    11401 aacctagctc gttttcaacg agaacaactt tgagtttggg gaaacgttcg agcacaccgg
    11461 aatagatcag gtcatagagg ctatcgaccg actgggccag cttcgagttg acgctattgt
    11521 gctcggcggt cattccggtg gcggtatcgc cgagcccgaa ggaacggttt cggctataac
    11581 cgtggccgct caagatgtgc aggttgaccg gcatctcgag atcctgagag gcggcccaga
    11641 acttgtcgta atgcgatgac gagaacggca gctctggcgg cggcacttgc cagatcaccg
    11701 agccgcgcat gccggctttg cggcagcgct ccagttcctt aacggcgccg tcgctgtcgt
    11761 acatcgacag catggcgatc ccgatcattc gctcgggcgc ggcgctgcag tagtcgatca
    11821 gccagtcatt atagacccgg cagcaggccg cctccaactc gtgatcgtcg aggctcaagc
    11881 atttcaagcc atgcgtcgga tagagcactt cggcgctgac gccgtcggcg gccatctcgg
    11941 aaacgcgctt gttcttgtcc tgggcgccgc cgggctggtc gttcggtctt tgcccgccgt
    12001 aatccggagc ccgatcctga aaagctttgg gcagttgttt ctgccaaagc cccgcgggct
    12061 cgatgacgtg ggaatccgat gaaatcaaca attcattgat agcggcgtta gtctctttgc
    12121 cataggtgcg ttcaataata gccatgatct tggctccttt tgtgtttagg gtgtgaggtc
    12181 gttgattcta aaagtccgct gagaggcatt aataaaggca taacccagtc gagggtcacg
    12241 gcgcaagcga aggatcgtca atcgcgcggg gatcggagaa aataccggct gcgcctttaa
    12301 cattttgcgg ttatccgaac cgtgaccgat gatcaaatcc tacgttcttt cttatcaagc
    12361 tccttccgat tccgaatacc ctgctaaaga tagggagcgt taagattgaa gacacggatc
    12421 ggggtcgagg cgtttgactc aacgagcggg tccattgcag agatttaggc tggatgactg
    12481 attttggctg atcaacccgc ccgcatcttg agcagagtcg gaatatccat tgtttcctga
    12541 tagcgccgca attctttttc tttgctccgc accgccagca tggccatgcg gtcggcgagt
    12601 tcgttaccct cggtgccgac gtgcccg
//
LOCUS       DASUVS010000427        12353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_408849, whole genome shotgun sequence.
ACCESSION   DASUVS010000427 DASUVS010000000
VERSION     DASUVS010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12353
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408849"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VF182_22495"
     CDS             <1..784
                     /locus_tag="VF182_22495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX6439905.1"
                     /translation="WPANRRIMYNRASADLQGKPWSERKKYIWWDGQKWTGYDVPDFA
                     ATKPPTAKAQPMGIGLDAHDGTDPFIMLDGGVGWLYVPTGLVDGPLPTHYEPAESPVQ
                     NPLYKQQSSPVLKYWKTPGNPLAPAGDAKYPHVITTYRLTEHYLAGAMSRWNPWLTEL
                     QPELFIELSPELAQEKGIQNLDWVRISSPRTQIRAKALVTRRMRPLQINGKTVHQVGM
                     PWHWGYEGLSTGDVVNELTALVGDPNVSIHEGKAFVCNVEKA"
     gene            876..1673
                     /locus_tag="VF182_22500"
     CDS             876..1673
                     /locus_tag="VF182_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6439906.1"
                     /translation="MAEPMGFYTDTTVCIGCKACEVACKEWNQLPATNGGLNTLSGDS
                     YDNTRRLDGIHWRHVKFVEQFTEDRGEGRWLLMSDVCKHCVQAACLEVCPTNAIIRTE
                     FDTVVIQSDACNGCRDCITACPFGVIDINPASGTAQKCTLCYDRLQSGMEPACSKACP
                     TDSIQFGTISDLRGRAQKRVEQLHQGGEKRAYLYGADEKMLGGLNSFFLLVDKPEVYG
                     LPPDPKMPTRNLASGSLFSVLGAVLVGLIGIFNFRNRGTREREERRL"
     gene            1670..2575
                     /gene="nrfD"
                     /locus_tag="VF182_22505"
     CDS             1670..2575
                     /gene="nrfD"
                     /locus_tag="VF182_22505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX6439907.1"
                     /translation="MNGIPSSTYFTVSPDWGWLIVFYFFFGGLAGGCYFLAVLIDFFG
                     RPEDRPLARLGYLISFPCVLISGILLTVDLHRPERFWHMLIESNTLQPMFKYWSPMSL
                     GSWALLIFGIFSFLSFLSALADSGRLNWAAARRFGRPGTIGGIISFIGGLFGFYVAGY
                     TGVLLAVTNRPIWSDSPLLGMLFVISAASISAALMILLAERNRWTMPGIADLHRMDMW
                     VLALEIIVLVAFLISLGPVFRAWFSIWGVVLLLGVVVLGLLVPLFLSWRRDWLGSYNF
                     TASAALVLIGGFILRMAIVLGAEAV"
     gene            2801..3676
                     /locus_tag="VF182_22510"
     CDS             2801..3676
                     /locus_tag="VF182_22510"
                     /inference="COORDINATES: protein motif:HMM:NF016131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HEX6439908.1"
                     /translation="MTVNEFEALAREYPDWQPWLLVVEEVMREATDASWETWVPTVES
                     QARDVPRLADISVTADLAKIRRWQQHLLQTAAQSGAPRMRQLDSLQKFALDSAALFEA
                     ALCENGAALKQMAEELEMDPEPFSAVTCLIAVPFLHACNRRWEQSRVPGWPAGYCPTC
                     GAWPAFAETRGIERSRYLRCGRCGAEWQAHALFCCFCGNSDHKELGSLVPENSGPSRA
                     VDTCSRCRGYLKTFTKLQGSAATKIMLDDLASVDLDIAALEQGFKRPPGPGYALNCKV
                     VARPSFGQRIFGRSV"
     gene            3673..4098
                     /locus_tag="VF182_22515"
     CDS             3673..4098
                     /locus_tag="VF182_22515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439909.1"
                     /translation="MSQSTLRPGDVCKALLAALEASEGRRRRRKRDQTPDSIGLTFKR
                     ELLEEAVRDDPEPEAFEEWLLNYTASHESAGGIFAMARAVFEEWRLAHSMGEFKSWLE
                     QGAPSDDAENVSSSATSDSHPGLMKGRSQHGQGRRTERT"
     gene            4067..4546
                     /locus_tag="VF182_22520"
     CDS             4067..4546
                     /locus_tag="VF182_22520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HEX6439910.1"
                     /translation="MAKGAEPNVRDRISDVFTRIEDITYIGLGVLLAAGALILLGAAA
                     WFLFNNIFSGDIRELIVDLLDQILLILMVVEILYTVQVSFREHTLSPEPFLVVGLIAA
                     VRRVLIITAEFSKPEEVLEAAFRNAMMELGLLTVLILSLVFSLHFLKRQSPARAERD"
     gene            4889..5341
                     /locus_tag="VF182_22525"
                     /pseudo
     CDS             4889..5341
                     /locus_tag="VF182_22525"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
     gene            5375..5752
                     /locus_tag="VF182_22530"
     CDS             5375..5752
                     /locus_tag="VF182_22530"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX6439911.1"
                     /translation="MKIATNLSLAVQMLAFSEGVLLAEKSGISRETAVEVLLNSVIAS
                     PMVKYRGPFVLNMPEEAWFDVKMMQKDLLLALEMGLKLDVPLPTTAITNQMLTSARAI
                     GLGKKDFAILFEVLAKMAGTKTA"
     gene            5849..6856
                     /locus_tag="VF182_22535"
     CDS             5849..6856
                     /locus_tag="VF182_22535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX6439912.1"
                     /translation="MARSKKHKAKPAKSASVQDERWIHFYRQMMKIRRFEEEVNQLYL
                     GAKMPGLAHLYIGQEAIAVGVCEALRRDDYITSTHRGHGHCLAKGAAVDKMFAELLGK
                     EAGYCRGKGGSMHIADQDTGNLGANAIVGGSAGIATGAALSIKMRGTDQVVVCFFGDG
                     ALGQGLLYEVMNMAALWKLPVIYVCENNQYNEYTNYRETTAGEVLGRAEAFGIHSQTI
                     DGQDVRVVHATSLELVQRARRGDGPAFLHCNTYRFHGHHVGDVDRSYYRSKKEEEHWK
                     TQRDPMRLLAEWLQKNKLADNNLLESIDKEISQEIISAVDLALAAPYPAAEEVDQHVY
                     A"
     gene            6993..7919
                     /locus_tag="VF182_22540"
     CDS             6993..7919
                     /locus_tag="VF182_22540"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX6439913.1"
                     /translation="MRRDPTVCIIGEDVAEAGTPFKVLSGLAAEFGTQRVIDSPISEA
                     GISGIGVGAAMTGMRPVVDIMFGDFITLTMDQLVNQAAKIHYMSGGKLKVPMVLRTTL
                     GATRRSAAQHSQSLHAWVSHVPGLKVVVPSTPYDAKGLLKSAIRDDNPVVFFEDKMMY
                     QLKGPVPAEEYTIPLGVADIKRAGNDITLVATSSMIQVALAAAAALAGDGIDAEVIDV
                     RTTAPLDTDTLVQSAKKTSRAIVIDEGYENYGVTAEIASVIADGAFYYLDAPVKRMGA
                     MNVPVPFSPVLEDQTVPTPEKVVKLARALCQK"
     gene            8068..9384
                     /locus_tag="VF182_22545"
     CDS             8068..9384
                     /locus_tag="VF182_22545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003996220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX6439914.1"
                     /translation="MGLKTFGLMAVDWEERVNIERLRQERLGRVKNALKKSEIGALLC
                     FDMNNIRYITATHIGTWAMDKLVRFSLLPQDDEPILWDFGSAARHHQIYCPWLGERSR
                     AGISTLRGATHPGSGLAEDVAKKIRLELEKRKLLNEPVGVDVIEIPVLFALQAQGIKV
                     VDGQQLMHEVRKIKTQDEITLLNTATMMVDAAYDELYRFMKPGVKENECVGVVSKVLY
                     DLGSEHVEGVNAISGERCSPHPHVYTDRVLRPGDPAFFDILHSFNGYRTCYYRTFAVG
                     SASPAMVDAYKRCRDYMDAAISIVKPGITTADVVKLWPKAEEFGFADEEAAFALQYGH
                     GVGLSIWEKPIFSRLVSLDHPEVIEEGMVFALETYWPASDGWSAARLEEEVVVTKDGC
                     EIITRFPSEKLLVAGTHYFTAGGELPTTRETQSALNNPGALERIKG"
     gene            9637..10836
                     /locus_tag="VF182_22550"
     CDS             9637..10836
                     /locus_tag="VF182_22550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX6439915.1"
                     /translation="MPTNVIMPALGVAQEKGTLINWLKSEGQTVNKGEPLMEVETDKA
                     TVEIEAAASGVLMNVTAAAGDEVPVGHTIAVILAPGETVPQKSVAQPLASSKENASVA
                     SGQVKPGVAMRTPSPKSRIPGSRILASPAARRIAREEGIDLASLSGSGPEGSVLIADV
                     RRGKQKHSLTLEDAPTVSGLLPLSPMRRIVGERMTQSKQSAPHFYISMDIDMTAIVAK
                     RSKWKESGERALPSINDFILSACAHTLKDFPILNARLSHEGIELVPEINIGMAVALEE
                     GLVVPVIRNADRLSLHDLAQQSWELSDKAQHKKLLPFDYEGGTFTVSNLGMLGVDHFI
                     AIINPPQCAILAVGRVAPRAVADEVGIAVRSMMTATLSADHRLVDGAIAARFLADFKG
                     RMENPDH"
     gene            10844..11629
                     /locus_tag="VF182_22555"
     CDS             10844..11629
                     /locus_tag="VF182_22555"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6439916.1"
                     /translation="MTRIVIRNLTKTYSGGNVVALQDINIDIEPYESICILGPSGCGK
                     TTLLRIIDCLIPRDGGEVIMNGAPVTRPRPDIAMVFQHFGLFPWKRLEQNIAYGLELR
                     GRPKHEAAATIARYLELMGLKGFEKSYPHQLSGGMQQRAGLARALAVNPSLLLMDEPF
                     GSLDAQTREMMQEELLRILETEKKTMIFVTHSIDEAILLGDRIVMMSRRPGRIQGVIP
                     VDIPRPRKIMSVRAHPRYIELRNTLWEMLTQNFASADTSKNAQ"
     gene            11626..>12353
                     /locus_tag="VF182_22560"
     CDS             11626..>12353
                     /locus_tag="VF182_22560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6439917.1"
                     /translation="MNRKIIVRLLSLIFVLLVWEYYGRRVNPVLFTYPSAIARAFLAL
                     VASGELQSYMKESLLVLAYASVLSVLAGVLLGVVMGRFSIVEWAADIYVNALYSTPMV
                     AVVPLIVLWFGFKVPAKVIIVFLFMVFPVLLNTYEGVKNVDRNLQEVARSFCSSESQL
                     WRHLIIPSAVPFIVAGVRLAIGRGLVGMIVAEFYTSITGLGYMIVRYANALETDKLFV
                     PIVVVMILGVGLLSLAKWVEGRIAP"
BASE COUNT         2653 a         3346 c         3617 g         2737 t
ORIGIN      
        1 gtggcccgcc aaccggcgca ttatgtacaa ccgcgcatct gcggacctgc agggcaagcc
       61 ctggagcgag cgcaaaaagt atatttggtg ggatggtcaa aagtggaccg ggtacgatgt
      121 gccggatttc gctgcgacga agccgccgac ggcgaaggcc cagcccatgg gcattggact
      181 cgacgctcac gacggaaccg acccatttat catgctcgat ggcggtgtgg gttggctcta
      241 tgtccccacc gggttggtag atgggccgct accgacccat tatgagccgg ccgagtctcc
      301 ggttcagaat cctctctata agcagcagtc cagtccggtt ctgaaatact ggaagacccc
      361 aggaaatcct ttggctccgg cgggagacgc aaagtacccg cacgtcatca cgacttatcg
      421 attgaccgaa cactatctgg ccggcgccat gagccgctgg aatccctggt tgacggaatt
      481 gcagcctgag cttttcatcg agctcagccc ggagttggcg caggaaaaag gcattcaaaa
      541 tctcgattgg gtcagaatat caagtccgcg cacccagatt cgcgccaagg ctttggtgac
      601 gcgccggatg cgaccgctgc agatcaacgg caagactgtc catcaagtgg gcatgccgtg
      661 gcactggggt tacgagggtc tttcaacagg cgacgtggtc aatgaattga cggccctcgt
      721 cggcgatccg aatgtctcga tacacgaagg caaagcattc gtttgtaacg tcgagaaagc
      781 ctgacggagt tttggagtac tggagtaatg cgctccgatc aacccaacac tcgttcactc
      841 cagcactccc ggcggggtta ggttaggaga taatcatggc cgagccaatg ggattctaca
      901 ccgacacaac cgtctgtatc ggttgcaaag catgcgaggt ggcgtgcaaa gaatggaatc
      961 agctacccgc gaccaatggc ggcctcaata cgctcagcgg cgacagctac gacaatacgc
     1021 ggcgcctcga cggcatccac tggcgccacg tcaaattcgt cgagcagttc actgaagatc
     1081 gcggcgaggg acgctggctg ctgatgagcg atgtctgcaa gcactgcgtg caagccgcat
     1141 gtcttgaggt atgcccgacc aacgcaatca tccgaaccga attcgacaca gtggttattc
     1201 agtcggatgc ctgcaacggt tgccgcgact gcatcaccgc atgtcccttc ggggttatcg
     1261 acatcaatcc ggcatccggc accgcccaga agtgcacgct ttgttacgat cgccttcaga
     1321 gcgggatgga gccggcttgc tcgaaagctt gccccaccga ttccatccag ttcggcacga
     1381 tcagcgattt gcggggcaga gcgcagaaaa gggtcgagca gcttcatcag ggcggggaga
     1441 aacgggctta tctttatggt gcagatgaga agatgttggg cgggctcaac tccttttttc
     1501 ttctcgttga caagccggag gtgtatgggc tgccgccgga cccgaagatg ccgacgcgaa
     1561 atctcgcttc cggttctctt ttctcagtgc ttggagccgt gttggttggt ttaatcggga
     1621 ttttcaattt ccgcaatcgc ggcacacgtg aacgcgagga gaggcggcta tgaacggcat
     1681 cccttcgagc acctatttta ccgtttcgcc tgattggggc tggctgattg ttttttactt
     1741 ttttttcggc ggactggccg gcggctgtta tttccttgcc gtgttgatcg attttttcgg
     1801 caggcctgaa gatcgtcctc tcgctcgact gggttactta atttcatttc cctgtgtcct
     1861 gatcagcggc attcttttaa ccgttgacct gcatcgcccg gagcgcttct ggcacatgct
     1921 tattgaatcc aataccttgc agccgatgtt taaatactgg tcgccgatgt cattgggttc
     1981 ctgggcgctt ttgatcttcg gcatattttc cttcctgtcc tttctcagcg ccctggcgga
     2041 tagcggccgc ctaaattggg cggcagcgcg ccggttcggg cgacctggaa ccatcggcgg
     2101 cattatttca tttatcggcg ggctctttgg cttttatgtc gccggttata ccggagttct
     2161 tttggcggtt acgaatcgtc cgatctggtc ggattcgccg ctgttgggca tgctgttcgt
     2221 tatttcggcg gcatctattt ccgcggcctt gatgatcctg ttggccgagc gtaaccgatg
     2281 gaccatgccc gggatcgcgg atttacatcg catggacatg tgggtgttgg cgctagaaat
     2341 tattgtcttg gttgcattct tgatttcact ggggccggtt tttcgcgcct ggttcagcat
     2401 ctggggcgtg gtgcttttgc tcggcgttgt tgtgttagga ctgcttgtgc cgttatttct
     2461 gtcatggcgt cgcgattggc tcggcagcta taatttcacg gcaagcgcgg cgttagtgct
     2521 gatcggcggt tttatcttgc gcatggccat cgtgctcggc gctgaggcag tctgacatga
     2581 agctcgcgct gttgataatt atcacggccg tcgttgccgg ctgtacgagc ccagaggcca
     2641 atcggagccg cggcggtgga ccgggtgggg atttgggaaa tcgaggttcg atcgtgcgta
     2701 tgcacgaagg atcgcgtcca tttgaacgaa caccgaaaat tatcggcgcc gagcatccgc
     2761 cgctagaatc cgcgcgccaa gccagcgagc tcagccgtcg atgacggtaa acgagttcga
     2821 ggctcttgcg cgcgagtacc cggattggca gccgtggctt ctggtcgtcg aagaagttat
     2881 gcgcgaggcg accgatgcga gctgggaaac ctgggttcca acggtcgaat ctcaggcccg
     2941 cgatgttcca cgtctagctg acatcagcgt aacagccgat ctcgctaaaa tccgccgttg
     3001 gcaacaacac ctcctacaaa ccgcggctca aagcggcgcg cctagaatgc gtcagctcga
     3061 ttcgttgcaa aaattcgcgt tggactcggc ggcgcttttt gaagcggcct tatgcgaaaa
     3121 cggcgcggcg ctaaaacaga tggctgagga gctagagatg gatccagagc cgttttccgc
     3181 cgttacctgt ttaatcgccg tgccttttct gcacgcctgc aaccggcgct gggaacagtc
     3241 gcgagtgcca ggatggccgg caggttattg ccccacgtgc ggcgcgtggc cggcatttgc
     3301 cgaaacgcgc ggtatagagc ggagccgcta tttgcgctgc ggccgttgcg gcgcggagtg
     3361 gcaggcgcac gcgctatttt gttgcttttg cggcaacagc gatcataagg aacttggttc
     3421 actcgtcccg gaaaattccg ggcctagccg cgccgtcgat acctgtagcc ggtgtcgcgg
     3481 ctatctcaag accttcacca aattgcaagg cagcgctgcc accaagatca tgttggacga
     3541 tctcgcaagc gtcgacttgg acattgcggc gttggagcag ggcttcaaac gcccgcccgg
     3601 accgggttac gcattgaatt gtaaagtggt agccagaccg agtttcgggc agcggatctt
     3661 tggccggagc gtatgagtca atcgaccctt cgtcctggcg acgtttgcaa agctctactg
     3721 gcggcactgg aggcctcgga aggacggagg cgcaggcgca aacgcgacca aacccctgac
     3781 agcataggct tgacattcaa gcgcgaactg ctggaggagg ctgtcagaga cgatccagag
     3841 ccggaagctt tcgaagaatg gctgctcaat tacactgcta gccacgaatc tgccggcggg
     3901 atctttgcga tggccagagc ggtttttgaa gaatggcgcc ttgcccattc tatgggtgaa
     3961 tttaaatcct ggctcgaaca gggtgctcct tccgacgacg ccgagaacgt ctcttcatca
     4021 gcgacctccg actctcatcc tggactcatg aaaggacgat cgcagcatgg ccaagggcgc
     4081 cgaaccgaac gtacgtgacc gcatatcaga cgtttttact cgcattgaag acatcaccta
     4141 tatcggtttg ggagtgttac tcgccgccgg cgccctaatc cttctcggcg ccgccgcatg
     4201 gttcttgttc aacaatattt tttccggtga tatacgggag ctcatcgtcg atctgctcga
     4261 tcagattctg ctgatcctga tggttgtcga gatcctctac acggtgcagg tgtcgtttcg
     4321 cgagcatacg ctgtcacccg aaccttttct cgtcgtcggg ctgattgccg ccgtgcggcg
     4381 cgttctcatc atcaccgccg aattttccaa accggaagaa gttttggaag ctgcgttccg
     4441 caacgcgatg atggaattgg ggctgctaac ggtcttgatc ctatcgctgg tgttctctct
     4501 ccatttcctc aaacggcaga gtccggcgcg ggctgagcgc gattgatccc gacgcattgc
     4561 gagtcaagct tcttttttga tgtaagttgc tcgttctacg gttgggaagt cgcgtacggt
     4621 cgggacatag ttcaggccgt gacacgtcca ttaatcttgt tcaaccggct attttgggat
     4681 acatactgcc gagcaatcca ctcatagtgt agtccatgtc aaacatattg ccggcgacga
     4741 ggaattggat tgaaacattg ggtattcgag cggcaacaat ttgcgttacg catagcggcg
     4801 atctttcata gaactcgtta aaagatgatc atcgcaatcg gaaagcgtag gtaagaagga
     4861 ggggtttacg gcagatctcg gttttgtcgg attaggagtc atgggaagcc gcatggtaaa
     4921 gcggctgctc gatgccgggc atcgggttac ggggcataac cgaacccgtt ccaaagccca
     4981 agctctcatt ggtcaaggca tgacctgggg cgacacgccg cgcgcggtag cagaatctag
     5041 cgagatcacg ttctccatgg tcagtgatac tccggcgttg cacgcggtta ccggcggcca
     5101 ggacggtatc ttggctggcc tctcgtcgat gaaaatttac atcgatatga gcactgtaag
     5161 ccctgctgcc agccgtgaca ccgccgagaa agttgctgct cgaggagcgc gtatgctgga
     5221 tgcgccggtt tccggcagcg tcatcacgtt agaagagggc aaattatcat ttatggtagg
     5281 aggcgatcgc gccgtctttg agcaggcacg acccattctc gaagccattg gacccaaggt
     5341 cacttacgtg ggcggcaatg gattggcagt atccatgaaa atcgcgacca atctaagcct
     5401 cgccgtgcaa atgcttgctt ttagcgaagg agtgctgctg gccgaaaaaa gcggcatctc
     5461 gcgggagacc gcggtcgaag ttctgctcaa cagcgttatc gcttcaccca tggtgaaata
     5521 ccgcgggccc tttgtcttga acatgcctga agaagcctgg ttcgatgtca agatgatgca
     5581 gaaggacctg ctcctcgctt tggagatggg tcttaagctg gatgtgccgc tgccgaccac
     5641 cgcgattacc aatcaaatgc tcacgagcgc gcgcgctatc gggctcggca aaaaagattt
     5701 cgcgattttg tttgaagtgc tggcaaagat ggcagggacg aagaccgcat gatgggatag
     5761 tggagtactg ggggaattgg gtaagacaag tcaagatctt tccatcactc cagtactcca
     5821 ttacttcaag acatttgacg acactctgat ggcgcgatcg aaaaaacaca aagccaaacc
     5881 agcaaaaagc gcatcggttc aagacgaacg ctggattcat ttctatcgcc agatgatgaa
     5941 gattcgtcgc ttcgaggaag aggtcaatca actctatctc ggcgccaaga tgcccggtct
     6001 tgcccatctg tatatcggcc aggaggcgat cgccgtgggc gtttgcgaag cgctgcggcg
     6061 agacgattac attaccagca cgcatcgcgg tcacggccac tgtctcgcca aaggcgccgc
     6121 ggtcgacaaa atgtttgccg aactgctcgg caaagaggcc ggatactgcc gcggcaaagg
     6181 cggctccatg cacattgccg atcaagacac cggcaatctc ggcgccaatg ccatcgtcgg
     6241 cggcagcgca gggatcgcca cgggcgcagc tctatcgata aagatgcgcg gcacggatca
     6301 ggtggtggtc tgcttttttg gcgatggtgc tttagggcaa ggcttgcttt acgaagtgat
     6361 gaacatggca gccctgtgga aactgccggt catctatgtt tgcgagaaca atcaatataa
     6421 cgagtacacc aactaccgtg agaccaccgc cggtgaagta ttgggccgcg ccgaagcttt
     6481 tggcattcac agccaaacga tcgacgggca ggacgtgcgt gtcgtacacg ccactagcct
     6541 cgaattagtc cagcgcgccc ggcgcggtga cgggccggcg ttcctacact gcaataccta
     6601 ccgtttccac ggtcaccatg tcggtgatgt cgatcgctcc tactaccgct ccaaaaagga
     6661 agaagaacac tggaagaccc aacgtgatcc gatgcgcctc cttgcggaat ggctgcagaa
     6721 aaataaattg gcggataaca acctgctcga aagcatcgac aaggaaattt cgcaagagat
     6781 tatcagcgca gtcgatttag cgttggcggc tccctatccg gcggccgaag aggtagacca
     6841 gcatgtctac gcctaggatc cctgcagcgt ctcctcgccg tgcggcagag gggaagaacg
     6901 tggggaaccc atcacaggca accatcacaa gtaacaagcc cggccgcgag atcaccttca
     6961 gccaagcgat taacgaggcg ctgtccgaag aaatgcggcg cgatccgact gtgtgtatca
     7021 tcggtgaaga tgtcgccgag gcgggcacac cgttcaaagt gctatcaggt ctggccgcgg
     7081 agtttggcac ccagcgcgtg atcgactcgc cgatctccga agcaggcatc agcggtatcg
     7141 gcgtcggcgc cgctatgacg ggtatgcggc ctgtggtgga tatcatgttc ggcgatttca
     7201 tcaccttgac gatggaccag ctcgtcaacc aggcggcgaa aattcactat atgtccggcg
     7261 gcaagcttaa agtgccgatg gtgctgcgaa cgactctagg cgcaacccgc cgttcggcgg
     7321 cgcagcacag ccagagcctt cacgcctggg tgagtcatgt tcccggattg aaagtcgtcg
     7381 tgccgtcgac cccgtatgat gccaagggac tgctcaaaag cgctatccgc gatgacaacc
     7441 cggtcgtgtt ctttgaagac aaaatgatgt accaactgaa ggggccggtt ccggccgagg
     7501 agtacacgat cccgcttgga gtcgctgata tcaaacgcgc cggcaacgat atcaccttgg
     7561 tcgcaaccag cagcatgatt caggttgccc ttgcggcggc agccgcgttg gcaggagacg
     7621 gcattgatgc cgaggtgatc gatgtcagga ccaccgcgcc gctcgacaca gacacgctcg
     7681 ttcaatcggc caaaaaaacc agccgcgcca tcgtcatcga cgaaggttat gagaactacg
     7741 gcgtcacggc agaaattgca tcggtgatcg ccgacggcgc cttttactat ctcgacgcgc
     7801 cggtaaagcg catgggcgcc atgaatgtgc cggtgccgtt ttcgcccgtg cttgaagatc
     7861 aaaccgtgcc gactccggaa aaggttgtga aattggcgcg agcattgtgc caaaaataac
     7921 gaccgattct gggatggccg acagcaatgg cttgcgggtc aacgccagca gttccaaacg
     7981 ttccactggt tcagtgttcc acgatatggt cggttcaaca cttgaaacga ttgaaacgat
     8041 tggaacggta gttcttggag gttagtgatg gggttgaaaa ccttcggctt gatggccgtg
     8101 gactgggaag agcgggttaa tatcgagcgg ctgcggcaag agcgactggg gcgagtgaaa
     8161 aacgcgctaa agaaatccga gatcggtgcg ctgctctgtt tcgatatgaa caacattcgc
     8221 tacatcaccg ccacacacat cggcacctgg gcgatggaca agctggtgcg cttctcactg
     8281 ctgccacagg atgatgagcc gatcctgtgg gacttcggct cggcggcgcg gcaccatcaa
     8341 atttactgcc cttggctagg cgaacggtcg cgcgccggca tctcgacctt gcgcggcgca
     8401 actcatccgg gctccggttt ggccgaagac gtggcgaaga aaatccgcct cgaattggag
     8461 aagcgcaagc tcttgaatga gccggtcggt gtcgatgtta tcgagatccc ggtgcttttt
     8521 gccttgcagg cgcagggcat caaggtcgtc gatggccagc aactcatgca cgaagtaaga
     8581 aagatcaaaa ctcaggatga aataaccttg ctcaacaccg caacgatgat ggtggatgcg
     8641 gcttacgacg agctttatcg attcatgaag ccgggagtca aggaaaacga atgcgtcgga
     8701 gtggtgagca aggtgcttta cgatctcggc tccgagcatg tcgaaggcgt caacgcgatc
     8761 tcgggagaac gctgcagtcc tcatccgcac gtctacaccg atcgcgtgct caggcccggt
     8821 gatccggcct ttttcgatat tttgcacagc tttaatggtt accgcacctg ctactatcga
     8881 acctttgccg tcggcagcgc gtccccggca atggtcgacg cctacaagcg ctgccgcgac
     8941 tacatggacg cggcgatcag catcgtcaag ccgggaatca cgacggcaga tgtcgtcaag
     9001 ctctggccca aggcggagga atttggcttt gccgacgaag aggcagcctt cgcgctgcag
     9061 tacggccatg gcgtcggctt atcgatctgg gaaaaaccga ttttcagccg ccttgtttca
     9121 ttggaccatc ctgaggtcat cgaggagggc atggtgttcg ctttggaaac ctattggccg
     9181 gcaagcgatg gctggtcggc agcgcgtctc gaagaagaag tggtggtgac caaagacggc
     9241 tgcgaaatca tcacccgatt tccatcggag aaactgctgg tcgccggcac gcattacttc
     9301 actgccggcg gagagttgcc gaccacgcgg gaaacgcagt cggcgttgaa taatccaggc
     9361 gctctggaac ggatcaaggg ctaacaggct gatgaaagag actcatatgg ttttgaacca
     9421 tgaattatga aatctgaaag ctgaaatgaa ttccgctttc agattgccac tttcatcctt
     9481 ctaattcgcg cgcgcagcat ctgagatctt ttttatcagc ttggaagtag aattttccgg
     9541 ggacgttaga ctggagtcag ttttagacga tttggaaccg ttggaacgat tggaacagcg
     9601 tgccttgcgc agattggaac ccttgaactg atttctatgc caacgaatgt catcatgcct
     9661 gcgctgggcg tcgcccaaga aaaaggcacg ctgatcaact ggctcaagtc cgaaggtcag
     9721 acggtgaata aaggcgagcc cctgatggaa gtcgagaccg acaaggcaac ggtcgaaatc
     9781 gaagcggcgg cctcaggagt cttgatgaac gtcaccgcgg ctgccggtga cgaggtgccg
     9841 gtgggtcaca ctatcgcggt gatcctggcg ccgggcgaga ccgtgcccca aaaatcagtt
     9901 gcgcagccgt tagcttcttc aaaggaaaat gccagtgtcg cttctggcca ggtcaagcct
     9961 ggggtggcaa tgcgaacccc gagccccaag tcgcggattc cagggtcgcg cattttagcc
    10021 tcgccggcgg cgcggagaat cgcgcgcgag gaaggaatcg atctcgcaag cttgagcggc
    10081 agtgggccgg agggcagcgt gctgatcgcg gacgtcaggc gcggcaagca aaaacattcg
    10141 ctgacactcg aagacgcgcc aacggtaagc gggctcctgc cgctcagccc gatgcgccgg
    10201 attgtcggcg agcgcatgac gcaaagcaaa cagagcgcgc cgcattttta tatcagcatg
    10261 gacatcgaca tgacggcgat tgttgcaaag agaagcaagt ggaaggagag cggagagaga
    10321 gcgcttccgt cgatcaacga tttcatcttg agcgcctgcg cccacacctt gaaagatttt
    10381 ccgatcctga acgcacgatt gagccatgag ggaatcgagc ttgtgccgga gattaatatc
    10441 gggatggcgg tggctctcga ggaaggtttg gtggtgccgg tcattcggaa cgccgaccgg
    10501 ctcagcctgc atgacctggc gcaacagagc tgggagttgt ctgataaagc acagcacaag
    10561 aaattattgc cgttcgatta tgaaggcgga actttcacgg tctccaacct cggcatgctc
    10621 ggggtcgatc atttcatcgc catcatcaat ccgccgcaat gcgctattct ggcggttggg
    10681 cgggttgcgc cgcgtgccgt cgccgatgaa gtcggcatcg cggtccggtc gatgatgacc
    10741 gccacattgt ccgccgatca tcggctcgtc gacggagcga tcgccgcgcg cttcttggca
    10801 gacttcaagg ggcgcatgga aaaccctgat cattgatttt tgcatgacac ggatcgtcat
    10861 tcgaaatctc accaagactt acagcggcgg caatgtcgtc gccctgcaag acatcaatat
    10921 tgacattgag ccctacgaaa gcatttgcat cttggggccg agcggctgcg gtaagacgac
    10981 gttgctgcgg atcatcgact gcctgatacc gcgcgacggc ggcgaagtga tcatgaacgg
    11041 cgctccggtc acacggccgc gtcccgatat cgcgatggtg tttcaacatt tcggtctgtt
    11101 tccgtggaag cggctcgagc agaatatcgc ctatggcctg gagctcagag gccgtccgaa
    11161 acacgaggcg gctgcaacca ttgcgcgcta tctcgagctc atggggctca agggttttga
    11221 gaagtcttat ccccatcaac tctccggcgg gatgcagcag cgcgccggac tggcccgcgc
    11281 tctggccgtc aatccatcgc ttctactgat ggacgagccc tttggttctc tcgatgcgca
    11341 aacccgtgaa atgatgcagg aggagctgct gcggattctg gaaaccgaaa agaagacgat
    11401 gatttttgtc acccatagca tcgacgaggc gattcttctc ggcgatcgca tcgtcatgat
    11461 gtcgcgccgt ccagggcgca tccagggagt catcccggtc gatatcccgc gcccgcgaaa
    11521 gatcatgtca gtgcgagctc atcctcgcta catcgagctc cgaaatacgc tgtgggagat
    11581 gctcacgcag aacttcgcta gcgccgacac gtccaagaat gcccaatgaa tcgaaagatc
    11641 atagtgcgtt tgctttccct gatattcgtt cttctcgtct gggaatatta cggcaggaga
    11701 gtcaatccgg ttctgtttac ttatccttcg gcgatcgccc gtgccttcct cgcgctggtc
    11761 gccagtggcg agctgcaaag ctacatgaag gaaagcctgt tggtgctggc ctatgcctcg
    11821 gttctttcgg tgttggccgg tgttctattg ggcgtggtca tggggcgttt ctcgatagtc
    11881 gaatgggccg cggacatcta tgtcaatgcg ctgtactcca cgcccatggt cgcggtggtt
    11941 ccgttgatag ttctctggtt cggtttcaaa gtccccgcca aagtcatcat cgtgtttctc
    12001 ttcatggttt tcccggtgct tctcaatacc tatgagggag tgaaaaacgt cgatcgtaat
    12061 ctccaggagg tcgcccgctc tttttgctcg agcgagagcc agctctggcg ccatctgatc
    12121 attccatctg ccgttccctt cattgtggcc ggcgttcgcc tcgccatcgg gcgcggtctg
    12181 gtcgggatga tcgtcgccga gttctataca tccatcaccg gactgggtta tatgatcgtc
    12241 cgctatgcca atgccctgga aaccgacaaa ctctttgttc ccatcgtcgt ggttatgata
    12301 ctaggagttg gcttactgtc gctggcaaaa tgggtcgaag gtcgcatcgc tcc
//
LOCUS       DASUVS010000428         3567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1721791, whole genome shotgun sequence.
ACCESSION   DASUVS010000428 DASUVS010000000
VERSION     DASUVS010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721791"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VF182_22565"
     CDS             <1..297
                     /locus_tag="VF182_22565"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the reduction of N-acetyl-5-glutamyl
                     phosphate to N-acetyl-L-glutamate 5-semialdehyde in
                     arginine biosynthesis; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX6439918.1"
                     /translation="LPEAGDGSAMWEVLSARYANEAFIQVEPLSDANAADEFTFDPQA
                     HNDTNRISLHVLTHPSGHVILMARLDNLGKGAAGVAIQNLNLMLGVPERTGLPV"
     gene            complement(257..1153)
                     /locus_tag="VF182_22570"
     CDS             complement(257..1153)
                     /locus_tag="VF182_22570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX6439919.1"
                     /translation="MPIEPILSKTDAANRNHTGQLFSPHSKIWQVDREMALLLAGGRA
                     LLLQLAHPKVAAGVAQYSRFQQNPLARLQRTMTAMWSIGFDESAKAYAALERVGNIHK
                     HVHGFVQANEATPRDTSYDARDPQLLMWVHATLIDSAIVAYDLFVKPLSLQEKARYYE
                     DSKKLTDLFDIPNDMVPQSLGEFARYFNDMLQGNTLAVGPVARSLAHDIVYPKPWILR
                     PLAPLFRVVTAGLLPERLRDAYGLKWNRSRENIFWLFARLFRLLLPLIPGPVRIVPNA
                     RAAEKRLGFPKRASRFSQERRA"
     gene            complement(1213..1869)
                     /locus_tag="VF182_22575"
     CDS             complement(1213..1869)
                     /locus_tag="VF182_22575"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX6439920.1"
                     /translation="MSRVIYRSWIIALVSGLVCLCTGPVGAAQSPSLKEILASLEKGP
                     VLGSRSAPVTIIEFSDFRCSFCKKFWADTLPRLKKSFFEKGTVQFVFRHFAILGKQSE
                     QAALASECAAEQDKFWQYHDKLFANQAAFGFSESKLKEYAGELRLQQAKFDECLTTGK
                     YKSKVEQETVTAAYLGGRGTPMFLVNEWRLVGAQPFETFAKAIDQVLEASNSRAKTSR
                     "
     gene            complement(1954..3165)
                     /locus_tag="VF182_22580"
     CDS             complement(1954..3165)
                     /locus_tag="VF182_22580"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6439921.1"
                     /translation="MWHSVPLTLFWFTYMASLGIYFPYFSLYLRENAGLSGTQLGLVL
                     AVPPLIGMVAQPFWGQVADRSGARSRVLAFLSLGTAAGYLALGSARDFDTILLATVTL
                     SIPGTALFPMMMSVTLAVLRDAGRHAFGRVRVWGTAGFFILVVGFPWILSRYRITYGV
                     DPATPGISQPGLEVMFPVTAALVFAAALIAFSLPQHGVVGVRAARGDWRELLRNIGFL
                     RFLLFALVAHFLLHGPMWFFPLFVRSRGGDLDMIRGMWILMLLVEIPLVLSTGSGLKR
                     LGARGLLTVGVLVGGLRWLLSALITDHHLLFAVQALHGVTVVGLNLGSPLYLDTVAPE
                     KLRSTAQGILSMVALGIAGITSNTAAGWLVDQGGTDLLYLICGIGSLVLAVLAGWILP
                     APNHRSDGSLA"
     gene            3244..>3567
                     /locus_tag="VF182_22585"
     CDS             3244..>3567
                     /locus_tag="VF182_22585"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX6439922.1"
                     /translation="MNCESSQIVHTIGHSTRTIGAVIELLKAHRIDLVVDVRRWPASR
                     RLPHFNREALNNALKLEGIDYIWRGDLGGFRKPASDSPNTAWRVGAFRAYADFMLSPG
                     FQTIMH"
BASE COUNT          904 a         1057 c          919 g          687 t
ORIGIN      
        1 ctgcccgaag ccggcgacgg ctcggcgatg tgggaagtcc tttcggcgcg ctatgccaat
       61 gaagccttca tccaggtcga acccttgagc gatgccaacg ccgcggatga atttaccttc
      121 gatccgcaag cccataacga caccaaccga atttcccttc acgtgctgac acacccttcg
      181 ggccacgtga tcctgatggc gcggctcgac aatctcggca agggcgcggc tggagtggcc
      241 attcagaatc taaatctcat gctcggcgtt cctgagagaa ccggcttgcc cgtttaggaa
      301 aacccaggcg cttctccgcg gcgcgcgcat tgggaacaat tcgcaccggg cctggtatca
      361 acggcagcaa cagacggaac agcctggcaa acagccagaa aatgttttct ctacttcgat
      421 tccacttcaa gccatacgcg tcccgcaatc gctcgggcag taatcccgcc gttaccaccc
      481 gaaacaacgg cgctagcggc cgcaaaatcc acggttttgg atagacaata tcatgcgcca
      541 gggatcgggc caccggccca acagcaagcg tgtttccctg aagcatgtca ttgaagtacc
      601 gggcgaactc gcctaaggac tgaggaacca tatcgttagg aatgtcgaac aaatcggtaa
      661 gttttttcga atcttcatag tagcgggcct tttcctggag cgataacggc ttcacgaata
      721 agtcgtaggc gacgatcgcg gaatcaatca gcgtcgcatg aacccacatc aagagctgcg
      781 gatcccgggc atcatacgaa gtgtcgcgcg gtgtagcctc attcgcctga acgaagccgt
      841 gaacgtgctt atggatattg cccacccgct caagcgccgc ataggccttg gccgattcgt
      901 caaacccgat cgaccacatc gcggtcatgg tgcgctgcaa acgggctaaa ggattttgct
      961 ggaagcggct atactgggcg actccggcgg caactttggg gtgagcaagt tgcaacagca
     1021 atgcccggcc gccggctaac agcaaagcca tttcccggtc gacctgccaa attttgctgt
     1081 gaggtgaaaa aagctgaccg gtatgatttc gatttgcagc gtccgtctta ctgagtatag
     1141 gctctatagg catgaagcga aaattattgt cctgcacgcc gctctccaac gagagtcaaa
     1201 cgattagaga actcaccgtg acgtcttagc tctactattg ctcgcctcca acacctgatc
     1261 gatggctttt gcaaacgtct cgaaaggttg cgccccgact aaacgccatt cattaactag
     1321 aaacatcggc gtgccgcgcc cgcctagata agccgcggtc acggtttctt gttctacttt
     1381 ggatttgtat ttcccagttg tcaggcattc gtcgaacttc gcctgctgca ggcgcaactc
     1441 gccggcgtat tctttgagct tggattcgct aaacccaaac gccgcttgat tcgcaaagag
     1501 cttatcatga tattgccaga acttatcttg ctccgcggcg cattccgacg ccagggccgc
     1561 ctgctccgac tgtttaccta atatcgcaaa atggcgaaag acgaactgca ccgtgccctt
     1621 ctcaaaaaag gattttttaa gccttggcag ggtatccgcc caaaatttct tacagaagct
     1681 gcaacgaaaa tcggaaaact caatgatggt taccggcgcg ctccgcgatc ctaataccgg
     1741 gcctttttcc aaagaagcca aaatctcctt cagagaaggg ctttgcgccg ctccaactgg
     1801 accggtacag aggcagacca gcccagacac taaggcgatt atccacgacc gatagatgac
     1861 tctcgacata agactccgat gctctaacat tctactaatt taagatatta caaccttctt
     1921 gatcgaacct aaatgaagga tcctttaatt gcttcaagcc aagctcccgt ccgatcggtg
     1981 attcggcgcg ggcaaaatcc atcctgccag aaccgccaaa accaacgagc cgattccaca
     2041 gataagatac aacagatccg tgccgccttg atcgaccagc cagcccgccg ccgtgttaga
     2101 ggtgatgccg gcgataccca gtgctaccat cgagagaatt ccctgagccg tggagcgaag
     2161 cttttccggc gctaccgtgt ccaaataaag cgggctgccg aggttgagtc cgaccaccgt
     2221 cacgccgtga agcgcctgta ccgcaaacag cagatgatga tcagtgatca acgcggaaag
     2281 cagccagcgc aggcccccga ccaaaacgcc cacggtaagt aacccgcgcg cgcccaaacg
     2341 cttaagcccg ctgccggtgg acaagaccag cgggatttcg acgagcagca tcaagatcca
     2401 catgccgcgt atcatatcga ggtcgccacc gcgcgaacgg acaaaaagcg gaaagaacca
     2461 catagggccg tgcaaaagaa agtgagccac gagcgcgaac aataaaaatc ttagaaagcc
     2521 gatattgcgc aaaagctcgc gccagtcgcc gcgcgcggcg cgcaccccga ctaccccgtg
     2581 ttgcggcagg gagaatgcga tcagcgcagc ggcgaatacc aaggcagccg tcaccggaaa
     2641 cataacttcc agccccggct gcgagatccc cggcgtcgca gggtctacgc cataagtgat
     2701 ccgatagcgg gaaagaatcc atggaaaacc aaccaccaag ataaaaaaac cagctgtgcc
     2761 ccacacccgc accctgccga atgcatgccg gccggcatcg cgcagaaccg ccagcgtcac
     2821 cgacatcatc atcggaaaca gcgccgttcc aggaatcgat agtgttaccg tcgctagaag
     2881 tatcgtgtcg aaatctcgag cggatcccag ggccagataa ccggcagcgg ttccgaggct
     2941 gagaaacgcc agcacccggc ttcgtgctcc gctgcgatcg gcgacttgac cccaaaacgg
     3001 ctgcgcgacc atgccgatga gcggaggaac cgcgagcacc agccccagtt gggtgccgga
     3061 aagacccgcg ttctcccgca gatagagact gaagtaaggg aaataaattc ccagcgaggc
     3121 catgtaggtg aaccagaaaa gcgtaagcgg aaccgaatgc cacatcgcga aagaataaca
     3181 tccctgtggt tggtgaacag ccaagactcg gttaaattta gaaatagacc gcgagaaaag
     3241 tcagtgaact gcgaatcctc acaaatcgtt catacgattg gccattcgac ccgaaccatc
     3301 ggcgccgtca tcgagctgtt gaaagcacac cgtatcgatt tggttgtcga cgtgcggcgc
     3361 tggccggcat cgcggcgtct tcctcatttc aaccgtgaag cgttaaataa cgcgctgaag
     3421 cttgaaggca tcgactatat ctggcgcggc gatctggggg gatttcgcaa accggcgtcc
     3481 gattctccga acaccgcctg gagagtcggc gcttttcgcg cctatgccga tttcatgttg
     3541 agtcccggat tccagaccat catgcac
//
LOCUS       DASUVS010000429         3640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_846929, whole genome shotgun sequence.
ACCESSION   DASUVS010000429 DASUVS010000000
VERSION     DASUVS010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846929"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            524..1402
                     /locus_tag="VF182_22590"
     CDS             524..1402
                     /locus_tag="VF182_22590"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439923.1"
                     /translation="MPETGLHETQTVKGLRDGLKESGYKEGENIIVEVEDVKGVRSQL
                     QGALDELVKKRVDVIFTTGTRGTQAAMATTKDIPIVFRHPADPVMLGLVKNANRPGGN
                     VTGVAGFSLDANQKRLEVLKQIFPDLRRIHIFYDANNRFSPDNFAAAEKSARQLKLEV
                     SEHAVKTAKEFKSSLAQMEVNPGDAIFQIADDLIESQASALLEEAKKRRMATMFHSEV
                     WASRGSLAAYGPNYYEMGRQAADLVDKILKGAHPKDLAVAHANKFDLAINLRTANSIG
                     LKIPPEVLKKADKVIR"
     gene            1475..1981
                     /locus_tag="VF182_22595"
     CDS             1475..1981
                     /locus_tag="VF182_22595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEX6439924.1"
                     /translation="MSVAEHKAKGKKSIRCFVITVSDTRSETDDTSGQTIRSMLAAEE
                     HQIAGYRIVKDEPAQIEALLDEALAAIEVDAVIVNGGTGIAPRDGTYEVVSSLLEKKL
                     DGFGEIFRYLSYLDIGSAAIMSRSAAGSARGKVLISLPGSKGAVTLAMEKLILPEIRH
                     MVSQLQGT"
     gene            1978..2223
                     /gene="moaD"
                     /locus_tag="VF182_22600"
     CDS             1978..2223
                     /gene="moaD"
                     /locus_tag="VF182_22600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEX6439925.1"
                     /translation="MKIKVKFFAILRERAGAAEITRDLEDGSTVADLWQALQQDFPKL
                     NVAGIRLLYAVNQNYVSPDFKLSDQDEVVFIPPVSGG"
     gene            2229..2669
                     /locus_tag="VF182_22605"
     CDS             2229..2669
                     /locus_tag="VF182_22605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003354345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX6439926.1"
                     /translation="MFRVTHETIDLQELVRFVSDPEAGAIATFIGTTRNHNDGRKVIA
                     LDYDGYPEMAEKELARIGEAARKQWEISRMAIVHRLGPVQITESSVMIAVSSGHRAAA
                     FAACRFAIEEIKRTVPIWKKEVFEGGEIWIGTQTGEPLAQKDEG"
     gene            complement(2713..3363)
                     /locus_tag="VF182_22610"
     CDS             complement(2713..3363)
                     /locus_tag="VF182_22610"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX6439927.1"
                     /translation="MAALLNWIMESIRSYGAWSVFIGVIIESVIVPIPSPLIIMGAGF
                     ILISPQLSFFQAFAPIVLQIVLPGSVASTLGAYIGYGIGYFGGKTLVDRWKGFLGFNW
                     SDVEAMERRFQGGRVNTTIFLLRALPIFPLSVISAAAGLLRLPLKQFSLWTFYGAIPR
                     CLLLGYLGWGFGETYQGFAKGLDKAEGIVSAILIVTIIGAIIWLRARVRGKMLEKS"
BASE COUNT          940 a          938 c          992 g          770 t
ORIGIN      
        1 acttccccgg gttgagacca cattcacaat tttgggaggc atcgccttcc gcgcttgaag
       61 tgattatgcg atgggatgag cccctccaaa aagcgtgacc tagctttcac cattccgaac
      121 tcacgcttac tcataatttg aactttaaaa cactccgttc cggccagaat tcgagcaagg
      181 cgcaaaatgg cccagcaact cggctataat ttcaacattc cgcctcaacc atctcacagt
      241 ggccaggccg gatggggctg agttatcgcg cattgtgccg caagacgcgc tcgcgccagg
      301 tcggcaagat gatgacgtgc tctgtccggt acatcattcg gcaaattccg atcgaagccc
      361 gcctcttatg atatagtcgg tgtaacccag cgatggggct caggaaggta gaagcatgga
      421 taatttgcgc tgcgcacgct tggctttaat agctattttt gtttttggtt tgtcgagctc
      481 agaagcacag cagcccgctc gaactcctcg aataggaatt cttatgcccg aaaccggcct
      541 ccacgaaact cagacggtaa aagggttgcg agacggtctc aaggaatccg ggtacaaaga
      601 aggcgagaat atcattgtgg aagtggagga cgtcaaaggc gtgcgctcgc aattgcaagg
      661 cgcgctcgat gagctggtga agaagagagt agatgtaata tttacgacgg gcacccgggg
      721 gacacaagca gcgatggcga caacgaaaga catacctatc gtcttccgcc atccggctga
      781 tccggtgatg ctgggattgg tcaagaacgc aaaccgtccc ggtggcaatg tcactggcgt
      841 cgcgggattt tcgctggacg ccaaccaaaa acggttggag gttttaaagc agatttttcc
      901 agacctccgg cgcatacata tattctacga tgccaacaat aggttctccc cggataactt
      961 tgctgccgca gaaaaatcgg cgcggcagct aaaacttgag gtgagcgagc atgcggtcaa
     1021 aacggctaaa gaattcaaga gctcgctggc tcagatggag gtaaacccgg gtgatgccat
     1081 attccaaatc gccgatgatc tgatagaaag ccaagcgtct gcgctcttag aggaggcgaa
     1141 aaagcgaaga atggctacta tgtttcattc ggaagtatgg gccagcaggg gaagcttggc
     1201 ggcttatggg cccaactatt atgaaatggg ccggcaggcc gccgatctgg tcgataaaat
     1261 ccttaaaggc gcgcacccga aggatctagc cgtggcgcac gcaaataaat ttgatctggc
     1321 aatcaacctt agaacggcca acagtatcgg tttgaaaatc ccgccggaag tattaaagaa
     1381 ggccgataaa gtcattcgat aggtcaattg aatacgacgc cgacggtgtt atacgctctt
     1441 attaggatct aacattccct agaggtgaat gaacatgtca gttgctgaac acaaggcaaa
     1501 gggtaaaaag agcatccgct gttttgtcat caccgtcagc gatacgcgtt cggaaactga
     1561 tgataccagc ggccagacga ttagatcgat gctggcggcg gaagagcacc agattgccgg
     1621 ctaccgtatc gttaaagacg agcctgccca gatcgaagcg cttctcgacg aggcgcttgc
     1681 cgccatcgaa gtcgatgcgg tgatcgttaa tggcggaacc ggtattgcgc cccgggacgg
     1741 tacttacgag gtggtctcga gtttactcga aaaaaagctc gatggatttg gcgagatttt
     1801 tcgctacctg agctacctgg atatcggctc tgccgcgatc atgagccggt cggcagccgg
     1861 atccgctcgt ggtaaagtgc tgatctcact ccccggctcc aaaggcgcgg tcaccttggc
     1921 tatggagaag ctgatcctcc cagaaatccg ccacatggtt tctcaactgc aaggaacatg
     1981 aaaatcaaag ttaaattttt tgccatcttg cgcgagcgcg ccggagccgc ggagatcacg
     2041 agagatctcg aagacggaag cacggtcgcg gatctttggc aggcactgca acaagatttt
     2101 cccaaattga acgtcgcggg gatccgcctg ctgtatgcgg tcaatcagaa ttacgtcagc
     2161 ccagatttca agctcagcga tcaagacgag gtcgtgttta ttccgccagt cagcggagga
     2221 tgaggccgat gtttcgagtt acccacgaaa ccattgatct acaagagctc gtgcgattcg
     2281 tcagcgatcc cgaggccggc gccatcgcga cgtttatcgg caccacccgc aatcataacg
     2341 acggccgcaa ggttatcgcc ctcgattatg atggatatcc ggaaatggcg gagaaagaat
     2401 tagcgcgtat cggggaggcc gcgcgcaaac agtgggagat ttcccggatg gctatcgttc
     2461 atcgcctcgg tccggtgcag atcaccgaat cgagcgtgat gatcgccgtt tcttcgggtc
     2521 atcgggcggc ggcgtttgcc gcctgccgat ttgccatcga agagatcaag agaaccgtgc
     2581 cgatctggaa aaaagaagtt ttcgaaggtg gagaaatctg gatcggtacg caaaccggag
     2641 aaccactggc gcagaaagat gagggatgac gggtgggatt cagctcttgc gcgagtattt
     2701 cctatgcttt gttcagcttt tctcgagcat ctttccacgc actctcgcgc gcagccaaat
     2761 aatcgcgcca ataatggtca ctatcaagat cgctgagaca ataccttcgg ctttatcaag
     2821 gcctttagca aagccctggt atgtttcgcc gaagccccag ccaagatagc ccagcaataa
     2881 acagcgcggg atggccccgt aaaacgtcca taagctgaat tgcttgagcg gcaaacggag
     2941 caatcccgcc gctgcggaaa tcaccgagag cggaaaaatc ggcaatgccc gcaataagaa
     3001 aatcgtggtg ttgacacgac caccctgaaa gcgtcgctcc atcgcttcga catcgctcca
     3061 attaaatcca agaaatcctt tccagcgatc gaccagcgtt ttaccgccaa agtagccgat
     3121 cccgtaaccg atatacgcgc cgagggtcga cgcgacggat cccggcagaa cgatttgtaa
     3181 aacgatcggc gcaaaggctt ggaagaagct taactgcggg ctgatcaaaa tgaaacccgc
     3241 gcccatgatg atcaacggcg acgggatcgg cacgattacc gactcgatga tgacgccgat
     3301 gaagacgctc cacgctccgt aagagcgaat cgactccatg atccaattta aaagtgcggc
     3361 cacgatttat ttctccccgt caagtctgcg ccagcgtaac agcttcaccg atttaagtta
     3421 agggaaccga ttcgaccctg tttccgccag gtccgccgca gagatcatcg ttatctgacc
     3481 gtcggcaatg gtccggtgcg gaatatgttg agcaaacagg gatggacgca atgccatttc
     3541 gtggggcggc caaatttctc cgagttaatc gatacgatga accgcgtctt gcggcagaga
     3601 ggcgagcagg gttttaagcg cgttatcgag gtaattgaaa
//
LOCUS       DASUVS010000430         6242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1504161, whole genome shotgun sequence.
ACCESSION   DASUVS010000430 DASUVS010000000
VERSION     DASUVS010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6242
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504161"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..473
                     /locus_tag="VF182_22615"
     CDS             123..473
                     /locus_tag="VF182_22615"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6439928.1"
                     /translation="MLFLDEVGELPLELQAKLLRVVQEGEFERLGSSKTIKVRARVIA
                     ATNRDLGLAVEDGTFRDDLYYRLNVYPIELPPLRDRKEDIPELARTFLSEASRRLGRS
                     FNGLSRRVIDGFMR"
     gene            1921..2196
                     /locus_tag="VF182_22620"
     CDS             1921..2196
                     /locus_tag="VF182_22620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439929.1"
                     /translation="MEKKSIARSLMIASIVGTFSFGYVCGSLNQRDAAAQVPGLGDAL
                     QRAGQSGGAVGNLSQLGSSIVEMQEHVNGLQKNIDTLKKFQSSLTGK"
     gene            2257..3390
                     /locus_tag="VF182_22625"
     CDS             2257..3390
                     /locus_tag="VF182_22625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6439930.1"
                     /translation="MSESGQLALRWANDVTLVVELSGDWRLRGGLPSASSVKHELELS
                     SKVRDVTFEARDLTSWDTSVLTFLVEVSELCRARAINMDRTGLPAGVQRLLELAEAVP
                     EKKGARKEEAKAPFFERVGNSVVGVGSALTEMLRFLGEMTVTFFRLFGMKARFRTVDL
                     LLLIQQSGAQALPIVTLISFLVGVILAFVGAVQLKQFGAQIYVADLVGIAMIREMGAM
                     MTGIIMAGRTGAAFAAQLGTMKVTQELDAFTTMGFAPFEFLVLPRVLALILMMPLLCL
                     YSDFLGVLGGATVGLGMLDLSWTVYMRQTANAITLIDVFGGVFKSSVYGILIALSGCL
                     RGMQCGNSSSAVGDAATSAVVTGIVAIVVACGTFAVVFYVLGI"
     gene            3497..4276
                     /locus_tag="VF182_22630"
     CDS             3497..4276
                     /locus_tag="VF182_22630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6439931.1"
                     /translation="MAGALANGAGAHIAVRNLTMAYGSFILMRDLNFTINHGDVFIIM
                     GGSGSGKSTLLRHLIGLNEPAQGKIFYGNESFTDADPVKRQEMLRRMGVLFQSGALWS
                     SMTLAENVGLPLGEYTDLTAAQIRDVASLKLALVGLKGFEDYYPSQISGGMQKRAGLA
                     RAMALDPEILFFDEPSAGLDPISSRLLDDLILELRASLGATVVVVTHELASIFTIGNN
                     SVFLDAETRTMIAEGDPKELLAHEQNPTVHRFLTRGEENPL"
     gene            4273..5283
                     /locus_tag="VF182_22635"
     CDS             4273..5283
                     /locus_tag="VF182_22635"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX6439932.1"
                     /translation="MSAKISPTIIGSFVVGALVLLIIAVIAFGSGRLFRQSKEFVLYF
                     DSSVNGLRVGAPVKLKGVEIGSVKDIRLQLEKELQVDKIPVIIEIDLEKLTSRGADSI
                     AAGDREAFRRMIVVRGLRGQLLMESVVTGLLYVGLDLFPGTPINLVQKPEGNYKFTEL
                     PTLPTYLEQAQDTATKILAKLEEVDYQGLMNTVTAAIDGFTRLVDSPALKSSLNGLER
                     TIPKLEQAVLSIHKLAAGMDANTTALATNLERSSDVAREAMEQAAVAMKQTDAALKEA
                     QAVMINIREMSDPDSSTSYELNKSLREVSAAARSLRLLSGYVERNPRALLFGKPESGE
                     ER"
     gene            5280..5900
                     /locus_tag="VF182_22640"
     CDS             5280..5900
                     /locus_tag="VF182_22640"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HEX6439933.1"
                     /translation="MSRSIQAAIVLGFVILFFGGCSSFSARSDPSRFFTLSAIAVEDR
                     AASDATHREKISLGVGPVKFPGYLDRQEIVVRSAQNRFQVSEYERWAEPLDENFTRVL
                     AQNLAILLQTDWVVAYPWLSSRKPNFQIEIEVLRFEPNAARDVQLAARWLVIDGRNKQ
                     PLDLKESRVTRHVNQGSTEASVAALSEAVADLSREIANAVTAVMAR"
     gene            6018..>6242
                     /locus_tag="VF182_22645"
     CDS             6018..>6242
                     /locus_tag="VF182_22645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439934.1"
                     /translation="MSVFGVGNGEVQSRLNLASSQLVTERNKTPAHPLYGPILYHAPD
                     VDRHDPHDNLGCTGDLYSVASGEEIRDLLAL"
BASE COUNT         1398 a         1622 c         1785 g         1437 t
ORIGIN      
        1 acgcgagttc ctccgcatgg tggcaaagga aggctcttga accaagcgga tttcaaatga
       61 gctcgggagc atcaatgcgc cgcaaccaaa cgcaccggcc gctttgaatt ggccaatgag
      121 cgatactttt tcttgacgag gtcggcgaac tgcccttgga gcttcaagcc aagctgctgc
      181 gcgtggttca agagggcgaa ttcgagcgcc tgggtagctc gaagacgatc aaggtaagag
      241 cgcgagtgat tgcggctact aatcgcgatc tcggattagc ggtcgaggac ggaacgtttc
      301 gggatgattt gtattatcga cttaacgtgt atcctattga gctcccgcca ttgcgggatc
      361 gtaaagagga cataccggag cttgcccgga cgtttctcag cgaagccagt cgaaggcttg
      421 gtcgatcttt caacggccta tcgcgacgcg tgattgacgg cttcatgaga tagggcgact
      481 ggccgggtaa cgtccgtgag ttgcagaacg tgatagagcg tatggctgtc actacaaaac
      541 gaaaccagct aaaccttccg ggtgattgga tgagtcatgc gctaatcgaa atgacttctc
      601 gccgatctca ttctgctgtg gccatgggag gagaagctat aatagagaac gtcgaccgcg
      661 cccatatcct cagggtgctc gagcaaagta actggaaaat agaaggtccc gtgggcgctg
      721 ccgttatcct ggcgctaaaa ccaagtacat tgcgttcgcg gatgcgcaag ttaggtagat
      781 cgatcatccg cccaaaagta tcattaggtt cgccgttatg gtgcatctcg tccacttgtg
      841 catatcctag aacttctcag gcgcttgtcc tgagacaacg cgcggctgac gtgaactaga
      901 caagcaatca attaacggtt aaatccatgc tttcctaaac gagattcgct gtccccattc
      961 catttccatc gctcccaaat atagccacgg tatttagtgg ctctcacgac atgccgagga
     1021 tgatccccgg tgcgaggagt atcacggtcc ctaacaacgc cgattctcta ggagaattat
     1081 acatttatga aattgtggcc gccggactta ttgtttcttg gcgcgggcat tactcttgcg
     1141 ctagcagagt tcgaattttt gattcgtggg tgtgttcggt ttctgacaag tccagggtct
     1201 ttcaagcatc catggcggaa gcttttgtaa cggcgaaact gcacggggta tagtgcaact
     1261 gacccgagcc gtagatgcca aagtacgaaa acaaaccccc cggaagtagg gcaaagcatt
     1321 gcgtgaacaa tcgcgctcga tcaggatagc ggagaaatcc cgggacctct ggtaaaccgg
     1381 gaagaagtgt ttgttatttt ccggctcgta tatgcccgtg taagcgagcg ctcgcaaact
     1441 cgaacctaaa acgaggtcta aacatcatct caaagctcta attttcgcaa gcaagcaaaa
     1501 atcgccatca gtcttcgtct ccaccggagg ccattcaaaa agtacgggct cgacttcaag
     1561 aagctggtcg acgatacgcg ccgccgtttg gcccctgcag cacatcaaag aagaaaagga
     1621 ttctgttacg cagattgctt tcatgctcgg ctactcggac gcccgcgcgt tcaatcgcgc
     1681 cttcaagcgc tcgaccggca cagcaccgtt gtaatatcgt cgtcctcttg cgacgcaggc
     1741 ccggacattt atataaagcg aaagtggctt tggcggactg ccggttgagc cggtcgccgc
     1801 accaacctca gcctggaccg cgctgctgct gtccgttgga actaaaagtc atgccaattg
     1861 tagtcaatta gagacgtaga tcgagggcga aatatcgact aattaagcac ggaggatgtc
     1921 atggaaaaaa agagtatcgc tcgcagtctg atgatcgcca gcatcgttgg cactttttcg
     1981 tttggatatg tttgcggttc tctcaaccag agagacgccg ccgctcaggt gccgggtttg
     2041 ggtgatgcgc ttcagagagc aggacaaagc ggcggcgccg ttggaaattt aagccaactg
     2101 ggctcatcga tcgtagagat gcaagagcac gtaaacggac tgcagaaaaa catcgacacg
     2161 ttgaagaaat ttcaatcaag cctgaccggc aaatagcaac gtgtaagccc gtcacggacg
     2221 gcgagggctc gtccttgccg ttcattggct caaggtatgt ctgaatccgg ccagctcgcc
     2281 ctccgctggg ccaatgacgt cacgctggtg gtggagttat caggcgactg gcgcttgcgt
     2341 ggcggtcttc cctcggcgtc atcggtcaag cacgagctcg agctgtcgag caaagtgcga
     2401 gacgtcacgt tcgaagctcg ggatcttacg tcgtgggaca ccagtgttct aacttttctc
     2461 gttgaagtct ccgagctctg ccgcgcgcga gcgatcaata tggaccgcac cggcctcccg
     2521 gccggcgtgc agcgattgct ggagctcgca gaagcagtac cggagaaaaa aggcgcgcgc
     2581 aaagaggagg cgaaagcgcc tttctttgag cgggtcggca acagcgtcgt cggtgtcggt
     2641 tcggcactga cggagatgct gagatttctc ggcgaaatga cggtgacgtt ttttcgcctg
     2701 tttggcatga aggcccgctt ccgaaccgtg gacctgttgc ttctcatcca gcagtcgggc
     2761 gcgcaggctc ttccgatcgt cacgttgatc agtttcctgg tcggcgtcat cctcgctttt
     2821 gtcggagccg tgcaactcaa acaattcggc gcgcagatct atgttgccga tctcgtcggc
     2881 attgccatga tccgtgaaat gggcgccatg atgacgggta tcatcatggc cgggcgcacg
     2941 ggcgctgcct tcgccgctca actcggcaca atgaaagtga ctcaagagct cgatgctttc
     3001 accacgatgg gctttgcgcc gttcgagttt cttgtcctgc ctcgagttct tgctttgatt
     3061 ttgatgatgc cccttctctg tctttactcc gattttctcg gtgtcctcgg cggggcgaca
     3121 gtcggtctcg gaatgctcga tctctcttgg acggtctata tgcgccaaac cgccaatgcg
     3181 attacgctga tcgacgtgtt cggcggcgtt ttcaaaagtt cggtttacgg catcttgatt
     3241 gccctttccg gctgtttgcg cggcatgcaa tgcggcaaca gttcatcggc ggtaggcgat
     3301 gccgcgacat ccgcggtcgt cacaggaatc gtcgcgatcg tcgtcgcctg cggcaccttt
     3361 gccgtcgtgt tttacgtatt gggaatctga atcaaagctg aaagatgaaa gctgaaagat
     3421 gacaaaacga tgagcggccc gattttattt cagttttccg ctttcacctt ttcatatttt
     3481 cgaagcgttg aatgatatgg ctggcgcgct tgcaaatggg gccggggcgc acatcgcggt
     3541 gcgcaatctt accatggcct acggtagctt cattctgatg cgcgatctta acttcaccat
     3601 caaccatgga gacgttttta tcatcatggg cggaagcggt tccgggaaga gcaccctgct
     3661 caggcacttg attggtctca acgagccggc gcaggggaaa atcttctacg gcaatgaaag
     3721 ttttaccgat gccgatcccg tcaaacgcca ggagatgctc agacgtatgg gcgtgctgtt
     3781 tcaaagcggc gcgctgtgga gctcgatgac tctcgcagag aatgttggtt tgccattggg
     3841 cgagtatact gatttgaccg cggctcaaat tcgcgacgtg gcatcgctca agctcgcctt
     3901 ggtcggcctc aagggtttcg aagattacta tcccagccag ataagcggcg gcatgcagaa
     3961 gcgcgcgggg ctggcgcggg ccatggcgct tgatccggaa atcctgttct tcgacgaacc
     4021 ctcggcgggt ctcgatccga tcagctcgcg cttgctcgac gacttgatcc tggagctgcg
     4081 cgcgagtttg ggcgctactg tcgtggtagt gacgcacgag ctggcgagta tctttacgat
     4141 cggcaacaac agtgtatttc tcgatgccga aacgcgcacg atgattgccg agggcgatcc
     4201 gaaagagctg ctggctcacg agcaaaatcc tacggtgcat cgctttttga ctcgcggaga
     4261 ggagaatcca ctatgagcgc caagatcagt ccaacgatca taggaagctt tgtcgtcggc
     4321 gcgttggtgc tgctaattat cgccgtgatc gccttcggct ctggacggct atttcggcaa
     4381 agcaaggaat tcgtgctcta tttcgacagt tcggttaacg gactgcgcgt cggtgcgccg
     4441 gtaaagctca aaggagtcga gattggttcg gtgaaagaca tcaggttgca gctggaaaag
     4501 gagctgcaag tcgacaagat cccggtgatt atcgagatcg acctggagaa attgacgtcc
     4561 cgtggggcag actcgatcgc tgcgggggat cgcgaagcct ttcgcaggat gattgttgtt
     4621 cgcgggctga ggggacagct tctgatggaa agcgtggtta cgggattgct ttacgttggc
     4681 ctggatttgt ttccggggac gccgatcaac ctggttcaga agccggaggg taattacaaa
     4741 tttacagaac tgccgaccct gccgacttac cttgagcagg cccaggatac cgcaacgaaa
     4801 attttggcca agctcgaaga agtcgattac cagggtttga tgaatacggt cactgcagcc
     4861 atcgatggtt tcacgcggtt ggtggattcg ccggcactta agtcgtcgct taatggtctt
     4921 gaacgaacca ttcccaagct cgaacaagca gtccttagta ttcacaaatt ggccgccggc
     4981 atggatgcca ataccacagc tttagcgacg aatttggagc ggagctcgga cgttgcgcgc
     5041 gaagccatgg agcaagccgc ggtcgcgatg aagcagaccg acgccgctct caaagaagcc
     5101 caagcagtga tgatcaacat ccgcgagatg agtgatcccg attcttcgac ttcttacgag
     5161 ctcaacaaga gccttcgtga agtctccgcg gcagcgcgct cgttgcgtct cctgtcaggc
     5221 tacgttgaac gaaatcccag ggcgcttctt ttcggcaaac cggagtcagg ggaggagaga
     5281 tgagccgatc gatccaggcc gccatcgtgc tcggatttgt gattctattt tttggcggtt
     5341 gtagctcgtt ctcggcaaga tccgatccct cgagattctt caccttgtcg gcaattgcgg
     5401 tagaagacag agctgcatcg gatgcaaccc acagggaaaa aatctcactg ggcgtcgggc
     5461 cggtcaagtt tcccggttac ctggaccgtc aagagattgt ggtgcggagc gcgcaaaacc
     5521 gctttcaagt atccgagtac gaacgctggg ccgaaccgct ggacgaaaac tttacacgcg
     5581 tattggcgca aaacctggcg atcttgctgc agaccgattg ggtcgttgcc tatccctggc
     5641 tgagcagtag aaaaccgaac tttcaaattg aaatcgaagt gcttcgcttc gagcccaatg
     5701 ccgcgcggga tgttcagctg gcggcgcgct ggctggtgat cgacgggcgc aacaaacagc
     5761 cgctcgatct caaggagtcg cgcgttaccc ggcatgtcaa ccagggatcc accgaggctt
     5821 cggtggcggc gctgagcgaa gccgtcgcgg atctcagccg tgagattgct aatgcggtca
     5881 cggctgtgat ggcaagatga agtgatgcgg gttagctcga agcgcgcgat ggatttgctc
     5941 gttgggctga aatcgactgc cctgaataac tcaacgaccc gcgaagagag gagagtaatg
     6001 acgaaacaaa atcgatcttg tccgtctttg gcgtggggaa tggcgaagtt caatcccgac
     6061 taaacctggc aagcagccaa ttagtcaccg agcgcaataa aacacccgca caccccctgt
     6121 atggtccgat tctttatcat gctcctgacg tcgatcgtca tgatcctcat gacaatctgg
     6181 ggtgcactgg cgatttatat tccgtagcgt ccggcgaaga gatccgcgat ttgctggctt
     6241 tg
//
LOCUS       DASUVS010000431        17876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1064470, whole genome shotgun sequence.
ACCESSION   DASUVS010000431 DASUVS010000000
VERSION     DASUVS010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 17876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17876
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064470"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..639
                     /locus_tag="VF182_22650"
     CDS             121..639
                     /locus_tag="VF182_22650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6439935.1"
                     /translation="MAQPKLSLPLPFEEIMQRHQREIMRYLLRLSSNPEDAADLFQET
                     WLRAYRAYPRLDPDDSVRPWLYTIASNLWRNRARNSGRRARVLVPEEKQSSAADLFAK
                     NHRLNHENDGYAAVHLRELIAALPVKQQQALHLRYFAGLDYAAIADAIDCSEESARAN
                     VSQAMRKLKKRW"
     gene            645..1472
                     /locus_tag="VF182_22655"
     CDS             645..1472
                     /locus_tag="VF182_22655"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX6439936.1"
                     /translation="MKIETLMGSAAADTPEKLIEELLLRSSAKLDKALQRIRRPQAAV
                     GLVKTNLGNLLVAKSARGIVLNHYIEETTDVAAALSKLRLQFDPVEDQASVTAVGEEI
                     NRYLGGGAEALRHDADLALVSNPFQKKVLAKLQAVPRGALISYQGLGAAVGSASSARA
                     VGNALHNNPVPVYVPCHRVISSDGRIGGYGGGAGRKLQLLRSEGFALGDRAVRVPEDV
                     VWGHKTTRIYCRSRCRAAARAGRGRMLFFADHKAARQAGLRPCKICRPDEHFPETSG"
     gene            1522..2400
                     /locus_tag="VF182_22660"
     CDS             1522..2400
                     /locus_tag="VF182_22660"
                     /inference="COORDINATES: protein motif:HMM:NF017809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEX6439937.1"
                     /translation="MKKSFEITLPFKAPYDWPSIIQFYQSHSMPGVARVTGDSFARVF
                     RLGGEVGFVEIAPLVAESRLKVSIVPENPKTTSETLRRIRKMFDLDCDPGLVAKSFKR
                     IPLLTTLCRRFPGLRVARGWDCFETAICTILGQLVSAAHREHLIGQLVSNYGEQIIDP
                     STSKRAYLFPRAEILACSQLTAIGTTTARREAIRNFSRRVLSGAISLAEDQDPAAFRQ
                     ALLETKGLGPWSAEYIRLRTGDSDAFPKTDLILRRVLDLHSDLDLERIKPWGSYAAVY
                     LWKEFAQTLSRSRKRK"
     gene            2402..2902
                     /locus_tag="VF182_22665"
     CDS             2402..2902
                     /locus_tag="VF182_22665"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017131174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX6439938.1"
                     /translation="MMLFYKEMESPVGKLKLAASADALVAILWERESPNRIKLATLSY
                     DPQQPILIETERQLEEYFAGTRNEFNLPLEPAGTEFQKKVWRALREIPYGQTRSYLDL
                     AKRIGSAKAVRAVGAANGKNPLSIVVPCHRVVGANGALTGFAGGLEVKAKLLAHEGCR
                     RFSAQH"
     gene            2914..3630
                     /locus_tag="VF182_22670"
     CDS             2914..3630
                     /locus_tag="VF182_22670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX6439939.1"
                     /translation="MSVGGSNRAQTIDWERACAGLDERGFAVLHGALEGQICHEIIDY
                     YGDDYRFRSRIEMSRYSFGQGEYKYFNYPLPPIVQQLRTSIYPHLAALANRWIDRLGS
                     KLPRYPESLAEFLEQCHLAGQSRPTPLLLRYGPGDFNCLHQDLYGDVVFPFQLTFFLS
                     QHGRDFTGGEFVLVEQQPRRQSRVQVLYPEQGDAVIFSVHHRPVRGARGYYRANLRHG
                     VSTIQSGQRYTLGIIFHDAK"
     gene            complement(3812..4405)
                     /locus_tag="VF182_22675"
     CDS             complement(3812..4405)
                     /locus_tag="VF182_22675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX6439940.1"
                     /translation="MINPAGTICVFTGSRHGTHPEYADGARQLGRELVARNYGLVYGG
                     GNVGLMKVIADTVLELGGHVTGVIPDSLVTKEVAHRGLSDLRIVHSMHERKALMAELA
                     DGFIAMPGGIGTMEEFFEVLSWAQLGIHRKPCGLLNTAGYYDGLIDFLDHAVEHDFLK
                     PKHRSLLILANEPPEILRRLEEVIRTRNDQHFDSSKT"
     gene            complement(4483..4731)
                     /locus_tag="VF182_22680"
     CDS             complement(4483..4731)
                     /locus_tag="VF182_22680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439941.1"
                     /translation="MDWLDALQWPAMVVTVMAAWLIGSLRKIRRMIGFWCFLLSNALW
                     IVWGWHTRAWAMVILQIILAGMNIRGANKNETDNPAET"
     gene            complement(4837..5340)
                     /locus_tag="VF182_22685"
     CDS             complement(4837..5340)
                     /locus_tag="VF182_22685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX6439942.1"
                     /translation="MNVVWEELAAGFPDAETMARITIRLVVAMIFGAVIGIQRERAGK
                     PAGVRTHMLVSSGAAVFILASGEFGMNPDSISRVIQGLVTGIGFLGAGAILKLYDRRA
                     VEGLTTAAGIWMTAALGVAVGLGRFGLALVATLLAWMTLSLVRQLEHIMNRGDRKPED
                     DTISAED"
     gene            complement(5406..5837)
                     /locus_tag="VF182_22690"
     CDS             complement(5406..5837)
                     /locus_tag="VF182_22690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6439943.1"
                     /translation="MGNTINFEQLVQAAGDAIIAADPEGRILSWNPAAERIFGFAAGE
                     ALGQTLDLIIPERFRARHWDGYKQTMRTGQTKYGADVLRVPARHKDGRPLSIAFTVAL
                     LSRADGQIEAIAAIVRDETSRWNEERSMRERLRKLEEQQSK"
     gene            complement(5966..7183)
                     /locus_tag="VF182_22695"
     CDS             complement(5966..7183)
                     /locus_tag="VF182_22695"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6439944.1"
                     /translation="MPFSKLGLSTKVVDGVQAAGYTDPTPIQLRAIPVLMEGRDLIGS
                     AQTGTGKTAAFALPMISRLGQRGALRALILEPTRELAAQVETAIRDYGRFTNLRTVVL
                     YGGTGYGKQDQALRRGADIVVATPGRLLDQISRGMLRLDQVEILVLDEADRMLDMGFM
                     PDVRKIVEKCPRNRQTMLFSATVPPEIDQLCKWALNKPEKIEIGQRRSPAETVTHALY
                     PVDSEQKQELLEELLRRTDYDQVLIFCRTKNGADRVVRKLHQQGHAVAVLHSRRTQRE
                     REHALNGFRNGRYEVMVATDIAARGIDVAQISHVINFDVPHHPEDYVHRIGRTGRAQS
                     VGDAFTIMTGEDVQEIAAIERFIGQKIPRVKLENFNYSYTRLLDPNPKPIFGRGRGHG
                     APGKRSFGYGRRR"
     gene            complement(7570..7974)
                     /locus_tag="VF182_22700"
     CDS             complement(7570..7974)
                     /locus_tag="VF182_22700"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX6439945.1"
                     /translation="MPDQELNGSCLCRAVRYQMTPPFMRFSHCYCSRCRKATGGVRAT
                     NIAVPIDQFRWTQGEELIARFDLPEAKSFARQICTRCNCPVPHPSRDRQRVIVPAGTL
                     DDEPATKPTAHGHWSSRVSWVEIDESDLPCSD"
     gene            complement(8082..8819)
                     /gene="surE"
                     /locus_tag="VF182_22705"
     CDS             complement(8082..8819)
                     /gene="surE"
                     /locus_tag="VF182_22705"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX6439946.1"
                     /translation="MRILVANDDGIYSPGIAVLAEVASEFGEVRMVAPDVERSSAGHS
                     ITAMRPLSYRRTPIGNFEAYRVDGTPADCVALGIFNWDKVDVVLSGINLGSNLGNSIW
                     HSGTLAAAKQATLLGLRGIALSSTVTIDEPNFDLLKPSLKEVLALLLNDNKDLCLVNV
                     NFPDEKPKGLRWTRQSVRHYDGKIATGEDPFGRKHFWFSVVPLEETEEGTDRWAVEQG
                     FVALTPLRLDLTNEKELAAALTRYPLA"
     gene            8918..9835
                     /locus_tag="VF182_22710"
     CDS             8918..9835
                     /locus_tag="VF182_22710"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX6439947.1"
                     /translation="MKVTLIHNPDAGSDDLTCGEEPVELIRRAGHQLVYQSSKDSHWK
                     TVLNKPADIIAVAGGDGIVGAVAEVMIGKGTPIAVLPLGTANNIAKTLNLMDLSLAHL
                     INGWSAARRKKFDAGIATGPWGSAHFIEGLGIGLFTDMMTRLDARGNVDLAHLTEPDE
                     KITSVLEMLQARLLTCPEIELHVTVDGRDLSGEYILFEVMNIKSIGPNLFLAPDADPG
                     DGLFDVVCLTRGAQETLDRCLEESKEGNPSPTGLPVRRGKRIHIEWEGTPIHIDDQAW
                     PESGADFPPSLTAIDITVEPQSLEFLIPP"
     gene            complement(10262..10525)
                     /locus_tag="VF182_22715"
     CDS             complement(10262..10525)
                     /locus_tag="VF182_22715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439948.1"
                     /translation="MEVTIGLARSDYEKLLHDVSPQHSAYNVLRRSSELDRWADEKPF
                     SMCVVIECSEEEALSLYHAAKQHCPHVLRDIEYGLKSAAVRQP"
     gene            complement(10795..11382)
                     /locus_tag="VF182_22720"
     CDS             complement(10795..11382)
                     /locus_tag="VF182_22720"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6439949.1"
                     /translation="MNVVLRKYAAADLDDCVSVLADAFVSSPLHLSAFGKDRIEQNRR
                     FFRLALRNMFVGQAFVALVDGALCGYVHFRAWPYCLPAPEEIPNAAATLVKPLGEALP
                     KFIQWFARWCHLDPQEPHIHLGPIGVIPGSQRQGIGTALMKRYIDGLEEERSVGYLET
                     DRLENVQFYKKFGFVVKHEEVVIDTPTWYMSRPLQ"
     gene            11587..12498
                     /locus_tag="VF182_22725"
     CDS             11587..12498
                     /locus_tag="VF182_22725"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX6439950.1"
                     /translation="MKILRFDDDRIGVIKNDDKVVDVSDAISARKAKGPQRVIEEIIE
                     GWRKYRRRFEKIMNEQEGVPLSNVQVRSPLPRPSKCIAAFSNYIDRPERTADNFPNEY
                     FYKSPDLVGPDGSVELTDIPAAVVYQPEAELAFVIGKPAKNVSESKALDYVFGYVPFF
                     DISTRGMVRRTQFVPKGQDTHGCVGPWIITKDEIPDPHNIYVKSWTNGEPRQNYNTSA
                     MAHKIPNQIAWLSRFVKLNPGDIVATGTFHEGLKPMNSGDTIEIEFANMGRAKFQVKG
                     NSPRKDADWLPGKNQPQPPPGGGMHKV"
     gene            complement(12589..13362)
                     /locus_tag="VF182_22730"
     CDS             complement(12589..13362)
                     /locus_tag="VF182_22730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6439951.1"
                     /translation="MRASKLNGLLFFLGLLIVWELVAQAGLINPLIVPALSRILRVFS
                     DLVWSGQIPLQVAASMKRAAAGYVMAALVFIPLGILMGLSQSVYRLFEVIVEMLRPVP
                     PPVMIPVAMLFLGLEDGMKIFVIFFSCAWPILLNTLDGARNVDRVLLNTARIFGLSRW
                     KIIWNVILPAASPQIVTGLKVSLPITLILVVISEMVGSTDGIGYFILDSQRRFRVAHM
                     YAGMLTLAILGYALNQFFNLFYRLLMPWHIGWLRSKDGR"
     gene            complement(13364..14107)
                     /locus_tag="VF182_22735"
     CDS             complement(13364..14107)
                     /locus_tag="VF182_22735"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6439952.1"
                     /translation="MLFALLLLLWEFGSRLSPALQSYLPPVSRILRSLGELIISGQLV
                     RHLMITLSRFLQGYLLAASIAVILGIVLGYFRMAHSLFEAVIEFLRPMPSVAIIPVAV
                     LLLGIGDAMIVAVTVYASVWPVLINTIDGVRHLERTLIDTGRTFGLNRRKILWLVVLP
                     AAAPYIVTGLRISLSIALILVTTAEMIAGSRGLGFFILDQERSMNSSNMYAGVILVSA
                     LGYVLNRLFVLGEGWAMQWRRGMLARESI"
     gene            complement(14140..14919)
                     /locus_tag="VF182_22740"
     CDS             complement(14140..14919)
                     /locus_tag="VF182_22740"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005484729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6439953.1"
                     /translation="MALLEVQNLAKTYRQMGGDTTDAIGEISCKIEPGEFVSFVGPSG
                     CGKTTLLMSIAGLIAPSAGQVIIHGKEVSGPPPNLVLVFQDFNKSLFAWRSVLGNVRF
                     GLEARAHRSRRGEDKARSLIDLVGLKGFENHYPWELSGGMQQRVAIARALAYEPEVLL
                     MDEPFGSLDALTRLELEDTVLQLWEELRTTILFITHDIEEAVYLSDRILLLSQRPSRI
                     IEELPIDFARPRNQVITRSQPRFMEIRNRIYQRIRNESAPS"
     gene            complement(15128..16090)
                     /locus_tag="VF182_22745"
     CDS             complement(15128..16090)
                     /locus_tag="VF182_22745"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439954.1"
                     /translation="MIISRRHFLGKVVIGVAAAPWLGDLGWAQTKGKVGYMKIVDVAA
                     MFMAIEKGFFKSEGLELETVPMAGGAVIVQGVTSGDLQIGWTNVISLYQAYAEGFDFK
                     LVGPGASNGRGKNESHAILVTKASPIKSAKDLEGKTVAVNTLNNIVHLMAMAWIDKNG
                     GASNKVKFVEVPFPQMEATLSSGKLDAASIHEPFATAAIEKGGARVLAHPWGDVLARF
                     HIAGWFASEKWIEKNKQMTQSFIRAITRGADAIQADPEASRAAMIKWAGLNPALAGKI
                     GLPIFDKTLSEKDLQSTIDLTHKYKMISRAIKAREVISDLAPKA"
     gene            16411..17571
                     /locus_tag="VF182_22750"
     CDS             16411..17571
                     /locus_tag="VF182_22750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6439955.1"
                     /translation="MPEPEQTPSETWVKDFFFTWINRFSFFLAHHLTRPILPLYLVSF
                     GASSTVIGAVMAVFTITATLMRIPVGLLIDRIGRKPFLIGGVALFAAGNFGYMWAPSI
                     FLMLPFRMLHGVGWSGCTTAIATLAADIAPQKRRGELIGYAAMASSLGGAIGPVAGFA
                     LLQRFDFFGVFLGAAALLFVGLVLAALVKEPGHSLRSGRRELSWMDLLVIPETLLPAV
                     AVAFLSFGHGGILTFLPIHALKLGLENPGLWFGLYALCLLLSRPVAGPLSDRISRRAV
                     IIPGLILNLLGIGILALASSPAWLMAAAVVGGFGTGAAQPALMTVAVDQTSPQRRGQS
                     MAQYQCFYDLGIGLGSLTLGAFLDLVDQNFSLMYAATAIVALIGLSIYWKRS"
     gene            17578..>17876
                     /locus_tag="VF182_22755"
     CDS             17578..>17876
                     /locus_tag="VF182_22755"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX6439956.1"
                     /translation="MLIDAHVHLDKYGDLLDDALRQIERENIFTVATAMDVPSYDDLQ
                     EIGRRSTLVLPTFGIHPRRAPQYADRLRELGQYIETSPAIGEIGLDFHWVKDPSE"
BASE COUNT         4122 a         5030 c         4807 g         3917 t
ORIGIN      
        1 gaacttgctt gagcaagttc attcgtgatt ttttgctcga gagaaccaac cgagactggc
       61 catcttgata aagagcgtca ggccttttca cattcgttct tctgtggtgt tttaatacat
      121 atggcgcaac ctaaactatc cctaccgctt ccgtttgaag agatcatgca gcgccatcag
      181 cgggaaataa tgcgctacct gctgcgcctg tcgagcaatc ccgaagatgc ggccgaccta
      241 ttccaggaga cctggttgcg cgcgtaccgc gcttatccca ggctcgaccc cgacgacagc
      301 gtgcgcccgt ggttgtacac gattgcgagc aatctatggc gcaaccgcgc gcggaactcc
      361 gggcgacgcg cccgcgtgct cgtaccggaa gaaaagcaat cctccgcagc ggacttgttt
      421 gcaaagaatc atcgattgaa tcatgagaac gacgggtacg ctgcggttca tctgcgcgag
      481 ctaatcgctg ccctgccggt caaacagcaa caagcgcttc atttgcgcta cttcgccggc
      541 ctcgattatg cggcgatagc ggatgccatc gactgttcgg aagaaagcgc ccgggcaaat
      601 gtttcgcagg cgatgagaaa gctcaagaaa agatggtgaa tatcatgaaa atagaaactc
      661 tgatgggctc ggccgcggct gacactccgg aaaaactcat cgaagaattg ttgcttcgct
      721 cttctgctaa actcgataag gcacttcagc ggattcgcag accccaagcg gctgtcggtc
      781 ttgtgaaaac gaatcttggc aatctgctgg tagccaagag cgcccgcgga attgtcctca
      841 atcattacat cgaggaaaca accgatgtcg ccgcagctct ctcgaagctg cggttgcagt
      901 tcgatccggt cgaggatcaa gcgtctgtaa cggcggtcgg cgaggagatc aaccgctatc
      961 tcggcggcgg tgccgaagcg ctacgtcacg atgccgatct ggctctggta agcaatccgt
     1021 tccagaagaa ggtcctggcc aagctgcaag cggtcccccg tggagcgctg atcagctatc
     1081 aaggtttggg ggcagctgtg ggctctgcaa gcagcgcgcg ggcggtcggc aatgcgctgc
     1141 acaacaatcc cgttcccgtg tacgttccct gccaccgagt catttcatcg gacggccgga
     1201 tcgggggata cggcggcggc gccgggcgca agcttcaatt gcttcgcagc gaaggttttg
     1261 cgctcggaga tcgggcggtg agagttccgg aggatgtcgt atggggccat aagacaaccc
     1321 ggatttactg ccgaagcagg tgccgcgccg cggcgcgggc gggccgtgga cggatgcttt
     1381 ttttcgccga tcataaagct gcccggcagg cgggtttacg tccgtgtaaa atttgccgtc
     1441 cggacgagca ctttcctgaa acttctggat gaaatcgaca aatctcttaa tgagagcgcc
     1501 gtttagagaa gggcgggcca tttgaaaaag tccttcgaga tcacgttacc gtttaaagca
     1561 ccgtacgatt ggccctcgat catacaattc taccagtcac attctatgcc cggagtcgca
     1621 cgggtgacag gcgattcctt cgcccgagtg tttcggttag gcggcgaagt aggctttgtc
     1681 gagattgcac cgctcgtggc cgagtctcgc ctcaaggttt cgatcgtgcc tgaaaatccg
     1741 aagacaacat cagaaaccct gcgccggatt cgaaaaatgt tcgacttgga ctgcgatcct
     1801 gggctcgttg cgaagagctt caagcgaatt ccactgctga caaccctatg ccgccgcttt
     1861 cccggactca gagtggctcg tgggtgggac tgcttcgaga ccgccatctg caccatcttg
     1921 gggcaactcg tttccgctgc gcacagagaa catctaatcg gccaactggt gagcaactac
     1981 ggcgagcaga taatcgatcc ctcaacatcg aaaagagcat acttgtttcc acgagctgag
     2041 atcctggcgt gctcacaact aactgcgata ggcacgacca ccgctaggcg agaggcgatc
     2101 cggaacttca gccggcgcgt gctgagcgga gccatttctc ttgccgaaga ccaggatccc
     2161 gccgcgtttc gccaggcgct tctggaaaca aaagggctcg gtccctggtc ggctgaatat
     2221 atcaggcttc gaaccggcga cagcgatgct tttcctaaga ccgatttgat tctcaggagg
     2281 gttctcgatc ttcattccga tctggactta gaacggatca agccttgggg atcgtatgcc
     2341 gcggtttatc tgtggaagga atttgcgcaa acgctctcca ggagcagaaa aaggaaatag
     2401 catgatgctc ttctataaag agatggaatc gccggtgggc aaactcaagc tggcggcgag
     2461 cgctgatgct ctcgtcgcga ttctgtggga gcgggaatcg ccaaaccgga tcaagcttgc
     2521 gacgctgagc tacgatccgc aacagccgat tctgatcgag accgagcgac agctagagga
     2581 atatttcgcc ggcacaagaa acgagttcaa tcttccgctt gagccggctg gaaccgaatt
     2641 tcagaagaag gtctggcggg cgttgcggga aattccctat gggcaaacca ggagttatct
     2701 ggatttggcc aagcgcatcg gctcggccaa ggccgtccgc gcagtcggcg ccgctaatgg
     2761 taaaaatccg ttatcgatcg tcgtgccctg tcatcgcgtt gttggcgcaa atggcgcgct
     2821 gaccggcttc gccggcggac ttgaagtcaa ggcgaagctc ttggctcacg aaggatgccg
     2881 gcgcttttcc gcgcagcact agaataatcc aatatgagcg tcggaggatc aaatcgagcg
     2941 cagacaatcg attgggagcg agcatgcgcg ggtctcgacg aacggggctt cgcggtcctc
     3001 cacggtgcgc ttgagggaca aatctgccac gagatcatcg actactacgg cgacgattat
     3061 cgctttcgct cgcgaatcga aatgtcccgt tactctttcg gccagggtga gtacaaatac
     3121 ttcaattacc cgctaccgcc gattgttcaa caactcagga cttcgatcta tccgcatctc
     3181 gccgcgcttg ctaatcgatg gatcgatcgg cttggaagca aattgccgcg ctatcctgaa
     3241 agccttgcgg agttccttga gcagtgtcat ctcgcgggcc aaagccggcc aacgccattg
     3301 ctgctgagat atggccccgg tgatttcaat tgcctgcacc aggatcttta cggtgacgtg
     3361 gtatttcctt ttcaacttac ctttttcttg agccagcacg gacgcgactt caccggcggt
     3421 gaattcgtgc ttgtcgaaca gcagccgcgc cggcaatctc gggtccaagt gctctatccc
     3481 gagcagggtg acgcggtaat cttttcagtt catcatcgac ccgttcgagg cgcccgcggc
     3541 tactaccggg ccaaccttcg tcacggcgtc agcaccattc agtcgggcca gcgatatacc
     3601 ctgggaatca ttttccacga tgccaagtga gagtgatcgc tgctcgactt gatcgctgaa
     3661 gcgaactcac aaagacgcgc tggaaatatt cgagaccgct tctgtgtgct ttagctgatg
     3721 ccaagacttg cgccccatgg aaccgcaata gactggtgcc taacacaaac aaccgcaggc
     3781 attggcagtg ccaatgcggc ttcgttgtgg cctaggtttt actggaatcg aagtgttggt
     3841 cgttacgagt ccggatgact tcttcgagcc ggcgcagaat ctccggcggt tcgtttgcca
     3901 ggatcagcaa tgaacgatgt ttcggcttga gaaagtcatg ctccactgca tggtcgagaa
     3961 agtcgatgag accatcgtaa tagccggcgg tgttcaggag tccacaaggc ttgcggtgaa
     4021 tgccaagctg agcccagcta agcacttcga agaactcctc catcgtgcca atgccgccgg
     4081 gcatcgcgat gaagccgtca gctaactccg ccatcaaggc tttgcgttcg tgcatcgaat
     4141 gcacaatacg cagatccgag agaccacgat gcgccacctc tttagtgact agcgaatcgg
     4201 ggattacacc ggtgacgtgg cctcccagct ccagaacagt atcggcgatg actttcatca
     4261 aaccaacatt accgccgccg taaaccagac cataattgcg cgcgaccaac tcccggccca
     4321 gttgtcgggc tccgtctgca tattccggat gagttccatg cctggagccc gtgaatacgc
     4381 aaattgttcc tgctggattg atcatgtgct cagatgtggc cgatccttcc ggagcttctg
     4441 agacctaaca ctgcgacgga tgaatctctg attgtagtta aactatgtct ccgccggatt
     4501 atccgtttca ttcttgttgg cgcctctgat attcatgccg gcaaggatta tctggagtat
     4561 caccattgcc caagcccgcg tgtgccagcc ccagacgatc caaagcgcat tgctcaacaa
     4621 gaagcaccaa aaaccgatca tccggcgaat ctttcgcagg gagccaataa gccaggcagc
     4681 cataaccgtc acgaccatcg ccggccactg aagagcatct agccaatcca tctatactgt
     4741 ccatcacttg agaaaacgac gttcttgctg aacaccgtga gatagcaatg attgccggtg
     4801 ctacggcgac tgaactgcca cgattgtcaa tgcgccttaa tcttcggccg aaatcgtgtc
     4861 gtcctcgggc ttccgatccc ctctattcat tatgtgttcg agttgacgca ccagtgagag
     4921 cgtcatccat gccagaaggg ttgccacgag cgccaggccg aaacggccca aaccgactgc
     4981 cacgcccaac gccgcggtca tccaaatgcc cgcagccgtg gtgagtccct cgacggctct
     5041 cctgtcgtag agctttagaa tcgccccggc gccgagaaag ccaattccag tcaccagacc
     5101 ctggatgaca cgtgaaatac tgtctgggtt catgccgaac tcgccgctgg ccagaataaa
     5161 aaccgccgcg cctgaagata ccaacatgtg ggttctaaca ccggcgggtt ttccggcgcg
     5221 ctcgcgttgt atgccgatca cggcgccaaa gatcattgca acgaccagcc ggatggttat
     5281 gcgggccatc gtttccgcat ccggaaatcc ggctgctaat tcttcccaga caacgttcat
     5341 aggcgccagc tcgaatttaa gagctccgac catgcgcgaa gcagagctac cccggctagc
     5401 gaaaattatt tagattgctg ctcctccagc tttcgcagtc gctcgcgcat tgagcgctct
     5461 tcattccaac gggaggtctc gtctcgaacg atagcagcga tcgcttcgat ctgtccgtct
     5521 gcccgtgata aaagcgccac cgtgaaggcg attgataaag gccggccatc tttgtgccga
     5581 gccggcaccc gcaaaacgtc cgcgccatat ttggtctgcc ccgtgcgcat cgtttgtttg
     5641 tagccgtccc aatgacgcgc gcgaaaccgc tccggaatga tcaaatccag ggtttgtccc
     5701 agcgcttcgc ccgctgcaaa accgaagatc cgttccgctg cggggttcca tgagaggatc
     5761 cggccttccg gatcggcggc aatgatcgca tcgcccgcgg cctgaaccaa ttgttcaaaa
     5821 ttaattgtat tgcccatggc tgtctccaac agttaacccg gcagatccat cgctgtatga
     5881 acctcacaag tgcgggtgac aacgattttt cccagaaccg tagccgaata atagctactg
     5941 atcagcacgg gcctggcgcg gcatcttatc ttcgtctgcc gtagccgaat gaacgcttac
     6001 cgggtgcgcc atgaccgcgg ccacggccaa aaatgggctt cggattggga tcgagaagtc
     6061 gggtatagga gtagttgaag ttctccagtt tgacccgcgg gatcttctgg ccgatgaagc
     6121 gttcgattgc ggcaatctcc tggacgtctt ccccggtcat aatggtaaag gcatcaccga
     6181 cactctgcgc gcggccggtg cggccgatcc ggtgcacgta gtcctccgga tgatgcggca
     6241 catcgaagtt gataacatgg ctgatctggg ccacgtcgat gccgcgggca gcgatatcgg
     6301 tagcgaccat tacttcataa cggccattgc gaaagccatt caaggcgtgc tcgcgctcgc
     6361 gctgggttcg gcgcgaatgc agaaccgcca ccgcatgtcc ctgctgatgg agcttgcgca
     6421 ccacccggtc cgcgccgttt ttagtccggc aaaatatcag cacctgatcg taatcggtgc
     6481 gccggagcag ttcttccaaa agttcctgtt tttgttcgct gtctaccgga tagagcgcgt
     6541 gggtgacggt ttccgcagga gagcggcgct ggccgatctc gatcttttcc ggtttattca
     6601 atgcccattt gcaaagctgg tcgatctccg gcggaaccgt tgccgaaaac aacattgttt
     6661 gacgattacg cgggcatttt tccacgatct tacgcacgtc gggcatgaat cccatgtcca
     6721 acattctatc ggcttcgtcg agtactagga tttcaacttg gtccaggcgc aacatgccgc
     6781 ggctaatctg gtcgagcaag cgacccggcg ttgccacgac gatgtccgcg ccgcgccgca
     6841 gcgcctgatc ctgcttgccg taacccgtgc cgccgtacaa aactacggtt cggagattgg
     6901 taaaccttcc ataatcgcga attgcggtct ccacctgcgc tgccaattcg cgagtcggct
     6961 ctaagatgag cgcgcgcaga gcgccgcgtt gtcccagccg cgatatcatc ggtaacgcaa
     7021 acgcggcggt ctttccggtg ccggtttgag ccgaaccgat gaggtcgcgg ccctccatca
     7081 ggaccggtat cgcacgtaat tgaatgggag tgggatcggt gtagccggcg gcctgtacgc
     7141 cgtcgacgac cttcgttgaa agtccaagct tggaaaaagg catagtcctt tatcacatgc
     7201 tatcgaagag ttaacaatcg caagacaaaa atcggcggct gaccgacgac aagtagaaca
     7261 gccaacagcg agaccgcggt tatactgact agcttcttcg ccatgttttt ggcaacggtt
     7321 tgtgctccga gttcatcatc ctggctctac gatccgccat cagatccgga aatggataac
     7381 cgactgaacg gttgtgcaac cgcttgcgtc atctccagtc ggcgcggtta gctcaccctg
     7441 atctgccgcg ctctagcctg ttcccaattt aatcacgtaa attccaagat gccgtcgcga
     7501 taagtcgcgc gaattccatt ctgctgaacg gtttgaagcg gtagaacggc gcttaaaaat
     7561 atgccgtgtt caatccgagc agggcagatc ggattcgtcg atctcgaccc agcttacgcg
     7621 gcttgaccag tgaccatggg ctgtcggttt tgttgcgggt tcatcgtcca aggtaccggc
     7681 tggaacgatg acccgctggc gatctcgtga cgggtgaggc acaggacaat tgcaacgcgt
     7741 acagatctgc cgcgcgaagc tcttggcttc gggcaggtcg aaacgcgcga tcagctcctc
     7801 tccctgagtc caacgaaact gatcgatcgg aacggcaatg ttggtcgctc tcacaccgcc
     7861 agtggctttg cggcagcgcg aacaatagca gtgagaaaat ctcatgaatg gcggtgtcat
     7921 ctgatagcgc actgctcggc aaagacaact tccattgagc tcttgatcag gcatgctgcc
     7981 tccctatgat tccggtcttg ggcttcacca tatttcgaac tgaccgcgcg ctgcaaggcg
     8041 ggctcccgcc attgaggaca acggcttgcg caacgtgaag atcacgccag cggataacgc
     8101 gtcaaggccg cggccaattc cttttcgttg gtcaaatcaa gccgcaacgg ggtcagagcc
     8161 acgaaacctt gctcgacggc ccagcggtcg gttccctctt cggtttcctc gagcggaacc
     8221 acactgaacc agaagtgctt gcgtccaaac gggtcctcgc cggtagcgat tttcccatcg
     8281 taatgacgca ccgactgccg ggtccatcga agccctttgg gcttttcatc gggaaagttc
     8341 acattcacca aacacaaatc cttgttgtcg ttcaacaaaa gcgccaagac ttccttcagg
     8401 gaaggtttca agagatcaaa gttcggctcg tcaatagtca cggtcgaact caacgcgatg
     8461 ccgcgtaagc ccagcaacgt cgcctgttta gcggcagcca gggtaccgga atgccaaatg
     8521 gaattgccca gattactgcc gagattgatc cccgaaagaa ccacgtcaac tttatcccag
     8581 ttaaagatcc ccagcgcgac gcaatcggcc ggtgtgccat cgacccggta agcttcaaaa
     8641 ttgccgatcg gagtgcgtct gtaggaaagc ggccgcatag cggtgattga atgtccggcc
     8701 gaggatcgtt ccacgtcagg cgccaccata cgcacttcac caaactcgct tgccacttcg
     8761 gccaaaacgg caattcccgg gctgtaaatg ccgtcatcat tggcaaccag gatacgcatg
     8821 agccagcctc ctcctctgag ctgcaagtta caaagtcgtt cagcagtgtt aatatgagta
     8881 tttcacattt gcccgcttcg gcaagataag gaaattcatg aaagtcacac tgattcataa
     8941 cccggatgcc ggcagcgatg atctaacctg cggcgaagaa ccggtcgagt tgattcgccg
     9001 cgccggccac cagctcgttt atcaatcttc aaaggacagt cattggaaaa cggtcctgaa
     9061 caaaccggcg gatatcatcg cggtagcggg tggcgacggc atcgtcggcg cggttgccga
     9121 ggtcatgatc gggaaaggaa cgccgatcgc ggttttaccg ttagggactg ccaataacat
     9181 cgccaagaca ctcaacctca tggacctttc actggcccat ttgatcaacg gatggagcgc
     9241 ggcgcggcga aagaaattcg atgccgggat cgccactgga ccctggggtt cggcgcattt
     9301 catcgagggt ctgggcattg gcctattcac cgacatgatg acccgcctgg acgccagagg
     9361 gaatgtggat cttgctcatt tgacggagcc tgacgagaaa atcacttcgg ttttggaaat
     9421 gttgcaggcc cggctattga cgtgtccaga gatagaactc cacgttacag tggacggccg
     9481 cgatctctcc ggcgaataca ttctgttcga ggtgatgaac ataaaatcca tcggtccgaa
     9541 tttgtttttg gcccctgacg ccgatcccgg cgacgggctg ttcgatgtgg tttgcctgac
     9601 ccgaggggct caggagacac tggaccgctg tctcgaggag tctaaggaag gcaatccgtc
     9661 gcccaccggc ttgccggtcc gaagaggaaa gcggatacat atcgagtggg aaggaacgcc
     9721 gatccatatc gacgatcaag catggcccga aagcggcgca gattttccgc cttcgttgac
     9781 ggcgattgat ataaccgtcg aacctcaaag tttggaattt ctgataccgc cctaaagcat
     9841 ttgcggcctc gggaaggcct cggttactgc taagcgatac ctaccgattt aatcagcgag
     9901 tcggcaaaac aaacttatct gagattcagt ttttggaagc cttgccgccg atgtgcttcc
     9961 agcctgcttc accgaattgc cggttccaga aagcaggcaa ccaatagggc gcctgacagc
    10021 tacggacgag ggcttcagtg aaaacggaca ctagtttgtc cggggggata gtagaagttg
    10081 aataaatcgt agggccgtcg aggatctctg ttcaggttct cgatgctctc ttggggaaaa
    10141 taaatcggca agctttcaga ttggtaagcg tgggattcaa gatctcgatt tctgcacaaa
    10201 cgcgctatag ccatcatgat ggcagccgat gccaatagtg ttttcatcct gactctcttt
    10261 cttatggctg gcgtaccgcc gctgacttaa ggccgtattc tatgtcgcgg aggacatgcg
    10321 ggcagtgctg cttcgccgca tgataaaggc tcagtgcttc ctcttcgctg cattcgatca
    10381 cgacacacat cgagaacggc ttctcatcgg cccaacgatc aagctccgac gaacggcgca
    10441 gaacattata ggcggaatgc tgaggggaga cgtcatgcaa cagtttttca taatcggatc
    10501 gcgctagccc aatggtaact tccataggtg tttacaactc aggaccactg agggtgcacc
    10561 ggagagcaat tcacgagcca acgtatgagt ttggcgatgg tcagtaaaag ctacaaataa
    10621 tcccggcagg ctgaagatgc gggaatggtg tagcgcagat ctggacgaac cagacaggca
    10681 gtgcaacaca aatgcgcaga atcggcggct tgctggcaga cttggtaatg atttctacag
    10741 ggctgaattc ttcttgtcag aaatctcgag taaatgtttg atcatcaccg gcgctcactg
    10801 tagaggtctg gacatgtacc aggtgggcgt gtcaataact acttcctcgt gcttgaccac
    10861 gaagccgaat tttttataga actggacgtt ctcaagtcga tcggtctcga gataaccgac
    10921 cgatctttcc tcctccagcc catcgatata acgcttcatc aatgcggtgc cgattccctg
    10981 tcgctggctt cctgggatca caccgatggg tccgagatga atatgcggct cctggggatc
    11041 aagatggcac catcgagcga accactgaat gaatttcggc agcgcctcgc ccagaggctt
    11101 caccaaggtc gcggccgcgt taggaatttc ctcgggcgcc ggcaggcagt aaggccaggc
    11161 tctgaaatga acatagccgc acagcgcgcc gtcgaccaac gcgacgaagg cttgaccgac
    11221 gaacatgttg cgcagggcga gccgaaagaa acgccggttc tgttcgatac gatccttgcc
    11281 gaaagcggag aggtgcaggg gactcgaaac gaaagcatcg gcgaggaccg acacgcaatc
    11341 gtctaaatcg gcggcagcgt atttcctcaa cacaacattc atgggcgcga agatccctag
    11401 cgaaagttta ctacgaactt tttcttcgag aaaacgtcac ggctccgaaa tgattgtctc
    11461 tctcttaaat tggagattgc cgtcaaaagc cgctcttctc tccgacctaa aatcatctga
    11521 gcgagattga cattactctg cggttgtcgc aaatataggc gcaaattgca ctggagggaa
    11581 cacaccgtga agattctacg atttgatgac gacagaatcg gggtgatcaa gaacgatgat
    11641 aaagttgtcg acgtcagcga tgccatcagc gcccgcaagg ccaaggggcc gcaacgggta
    11701 atcgaggaaa tcatcgaggg ctggcgcaag taccggcgcc gctttgaaaa gattatgaac
    11761 gaacaggaag gtgttccgtt aagcaatgtc caagttcgct cgcccctgcc acgcccgagc
    11821 aagtgcattg ccgctttcag caattatatc gatcgtccgg agcgtaccgc cgataatttt
    11881 cccaatgaat atttctacaa gtctcccgat ctcgtcgggc ccgacggttc ggtggaatta
    11941 accgacatcc ccgccgcagt cgtttatcaa cccgaagccg aactcgcatt cgtaatcggc
    12001 aaacccgcta aaaatgtctc agagtcaaag gcattggatt acgttttcgg ctatgtgcct
    12061 ttcttcgata tttcgacccg gggcatggtg cgtcgcacgc aatttgttcc gaagggacag
    12121 gacacccatg gttgtgtcgg gccgtggatc atcaccaaag atgaaattcc cgatccgcac
    12181 aatatttatg taaaatcgtg gaccaacggc gaaccgcggc aaaactacaa taccagcgca
    12241 atggcgcaca agatccccaa tcagatcgcc tggctatcgc gtttcgtcaa gctgaatccg
    12301 ggagacatcg tcgccaccgg cacttttcat gaagggctca agccgatgaa ttcgggcgac
    12361 actatagaaa tcgaattcgc caacatgggc cgggcgaagt ttcaagtcaa aggcaacagc
    12421 ccgcgcaagg atgcggactg gttgcccgga aaaaaccagc cacaacctcc cccgggaggc
    12481 ggcatgcaca aggtttaaga gaatgggtga aatcccattg gcgtgatgga gtactggagt
    12541 ggcggattta tccgcaacca tcactccacc actccaatac tccattcttc accggccgtc
    12601 cttcgatctc agccagccta tatgccatgg catgagtagc ctgtagaaaa gattgaaaaa
    12661 ttggttgagc gcgtagccga gaatcgccag ggtaagcatc cccgcgtaca tgtgcgctac
    12721 tctgaatcga cgctgcgaat cgagaataaa gtagccgatc ccatcggtgc tcccgaccat
    12781 ctctgagatg accacgagga taagcgtgat cggcaggctg accttcaatc cggtaacgat
    12841 ctgcggcgat gctgccggca gaatgacatt ccagatgatt ttccaacggg ataatccaaa
    12901 aatacgggcc gtgttgagca gcacgcgatc gacattacgg gcgccgtcca gggtattcag
    12961 aaggatcggc caggcgcagg aaaagaagat aacgaaaatt ttcatcccat cttccaaccc
    13021 gagaaacagc atcgctaccg gaatcatcac cggcggcggc accggccgga gcatctccac
    13081 gatcacttca aagaggcggt aaacactttg cgaaagaccc atcagaattc ccagaggaat
    13141 aaacactagg gcagccatga catagcctgc cgccgcccgc ttcatgctgg ctgcaacttg
    13201 taacggaatc tgtccggacc atactaaatc ggagaaaact cgaagaatcc tgctcaacgc
    13261 cggcacgatc aacggattga tcaatccggc ttgggcaacc aattcccaaa cgatcagcag
    13321 gccgagaaag aatagcaaac cgttaagctt cgatgcgcgc atgtcagatg gactcccggg
    13381 cgagcatacc gcggcgccac tgcatcgccc agccttcgcc aaggacgaac aggcgattaa
    13441 gcacatatcc cagagctgaa actaaaatga caccggcgta catattgctg ctgttcatgg
    13501 agcgttcttg gtcgagaatg aagaacccca gccctctgct gccggcgatc atctcggcag
    13561 tggtgaccag gatcagcgca atcgacagac tgatgcgtaa tccggtgacg atatagggcg
    13621 ctgccgcggg tagcacgacc agccaaagaa tctttcggcg attaagtcca aaggttctgc
    13681 cggtgtcgat caacgtccgc tcaaggtgcc ggaccccatc gatggtattg atgagaaccg
    13741 gccagacgct ggcgtagacg gtgaccgcga caatcatcgc gtcgccaatg ccgagcaaca
    13801 aaaccgccac gggaatgatc gccacggaag gcatcgggcg caagaactcg atgacggcct
    13861 caaacaggct gtgcgccata cggaaatatc ccaggacgat ccccagaatg accgctatcg
    13921 aagcggcaag caaataaccc tgaagaaagc ggctcagcgt aatcatcaga tgcctaacca
    13981 gctggcccga aataattagc tcgcccaagc tcctgagaat ccggcttacg ggcggaaggt
    14041 aggattgcag cgccggacta agccgggaac cgaactccca caggagcaaa aggagggcga
    14101 acagcatcca gcccgcccaa ttaaaatctc gtccaacgtt cacgacggcg cgctttcgtt
    14161 gcggatccgt tggtaaatgc gatttctaat ttccataaag cgaggttgtg acctggttat
    14221 cacttgattg cgcggcctgg caaaatcaat cggcagttcc tcgataatcc gcgacggccg
    14281 ttggctgagc aacaaaatac gatccgagag gtaaacggcc tcttcgatgt catgggtgat
    14341 aaacaaaatg gtcgtgcgca actcctccca gagctgtaga accgtatcct ccaactccag
    14401 gcgggtcagc gcatcgaggg agccaaaagg ctcgtccatc agcaaaactt cgggttcgta
    14461 agcaagcgct cgtgcgatcg cgacccgctg ctgcatgccg cccgaaagct cccatggata
    14521 atgattctcg aaacctttga ggccgacgag gtcaatcagc gagcgcgctt tgtcctcccc
    14581 tcggcgcgaa cgatgtgccc gcgcctctaa gccgaagcgc acatttccaa gcacggagcg
    14641 ccacgcaaat agagatttat tgaaatcttg gaaaaccagg accaggttcg gaggcggccc
    14701 actcacctct ttaccatgaa ttatgacttg tccggcgctg ggagcgatca gtccggcaat
    14761 cgacatcaac aaagtggttt tgccacatcc cgacggcccg acgaaactga caaactcgcc
    14821 cggttcgatc ttgcacgaga tctctccgat cgcatccgtc gtgtcgccgc ccatctgtcg
    14881 ataggtcttg gcaaggtttt ggacttctag aagagccatg aaagccaatt aacgatgaag
    14941 tagataccga aatgcgcaag ccacgaacaa caagcaccgg ataaaaccgg ccgcaacatc
    15001 aaatagtcaa gccttaagtg ctcgtcacat ttcaacagcg atgccttggc cgatttcctt
    15061 ttctccggcg catagtcgcc gggccatcag gctggtatgc gctcaagtcg ttttcctgcc
    15121 gaatgtatta agctttgggt gccaggtcgc tgatcacttc ccgtgcttta atcgcgcgtg
    15181 agatcatttt gtatttgtgg gtcagatcga tggtcgactg cagatccttt tccgataatg
    15241 ttttatcgaa gatcggcaga ccgattttgc ccgccagcgc aggattcaat ccggcccatt
    15301 ttatcatcgc ggcgcggctg gcttcgggat cggcctgtat ggcgtcggcg ccgcgcgtaa
    15361 tggctcgaat gaacgattga gtcatttgct tattcttctc gatccacttc tcagacgcga
    15421 accagccggc gatatgaaat ctagcaagca cgtcgcccca gggatgggcg agaactcgcg
    15481 ccccaccttt ttcgatggct gcggtggcaa agggttcatg aatactggcg gcatcgagct
    15541 tgccgctgct tagggtcgct tccatctggg gaaacggcac ctccacgaat ttgaccttat
    15601 ttgaagcgcc gccgttcttg tcgatccaag ccatggccat aagatgaacg atattgttga
    15661 gcgtattgac ggcgacggtt ttcccctcca gatctttggc gcttttaatc ggcgacgcct
    15721 tagtcaccaa aatcgcatga ctctcgtttt tgccgcggcc gttgctcgcg ccaggcccca
    15781 caagcttgaa atcgaatccc tccgcatagg cttgataaag cgagatcacg ttggtccagc
    15841 cgatctgcaa atcacccgag gtaactcctt gcacgatgac ggcaccgcct gccatgggca
    15901 ccgtctcgag ttctagaccc tcggacttga aaaatccttt ctctatggcc atgaacatcg
    15961 cggcaacatc gacgattttc atgtagccga ccttgccttt agtctgcgcc cagccgagat
    16021 cgccaagcca gggcgctgcc gccacgccga taacgacttt gccgagaaaa tgtcgccgac
    16081 taataatcat taaagtcctc ctgatagcta gactgaatcg tcgtttttac cacgaagaac
    16141 acgaaggtca cggaaaattc tttcgtttca ttctgcacat gatgagacat gaccgtttcc
    16201 tgcgggctct tcgtgacctt cgtggttttg ttagactcgc actagattcc gccgcccgga
    16261 tttctaataa acagatgaat gtgacgaact gttttccgtt tcaacgaacg caattacata
    16321 atccgaaatg aacccatcct gcaacaacag attgactttg attcagccct gccgcaatga
    16381 tgggcttttt taaattaaat ggcggcaatt gtgccagagc cggagcaaac accatcggag
    16441 acttgggtaa aagatttctt cttcacctgg atcaatcgat ttagcttttt tctcgcgcat
    16501 catctgactc ggccgatcct gccactgtat cttgtcagct tcggcgcttc ttccacagtg
    16561 attggcgccg tcatggcggt cttcactatt accgccaccc tcatgcgaat tcccgtagga
    16621 ctactcatcg atagaatcgg cagaaagcca ttcctcatcg gcggcgtcgc gctcttcgcg
    16681 gccggcaact tcggctatat gtgggcccca agcatttttt tgatgctgcc gttccgcatg
    16741 cttcacggcg tcggctggtc cggctgcaca actgcgatag cgacgttggc cgccgacata
    16801 gcgccgcaga agcggcgcgg cgagttgatc ggttatgccg ccatggcaag tagtctgggc
    16861 ggagccatcg ggccggtggc cgggttcgca ttgttgcaac ggtttgactt cttcggagta
    16921 tttctcggag cggcggcgct gttgtttgtc ggtctggtcc ttgcggcttt ggtcaaggag
    16981 ccggggcata gtcttcgaag cggccggcgg gaactaagct ggatggacct gctggtgatt
    17041 cccgagactc tattacccgc tgtggcggtc gcctttctat cattcggcca tggcggcatt
    17101 ctcaccttct taccaatcca tgcgctgaaa ctcggccttg aaaatcccgg actttggttc
    17161 ggtctctatg cgctttgcct tttgctcagc aggcccgttg ccggaccgct ttcagaccgg
    17221 atcagccggc gcgccgtcat cattcccggc ttgattctga acctgctcgg gatcggcata
    17281 ctggcgttgg cctcttcacc ggcatggctc atggcggcgg cagtggttgg aggtttcggc
    17341 accggcgcag cgcagccggc actcatgacc gtcgcggtgg atcaaacttc gccgcagcgg
    17401 cgcggccaat ccatggcgca gtatcagtgc ttttacgatc tcggcatcgg cctcggctcg
    17461 ttgacactgg gagcgtttct cgatttagtc gaccagaatt tctctctgat gtatgcggcg
    17521 acggcaatag tggcgctcat cggtttgtcg atttactgga agcggagttg atccgttatg
    17581 ttgatcgatg ctcatgttca tctggacaaa tacggcgatt tactcgacga cgcgttgaga
    17641 caaatcgaac gggaaaacat ttttaccgtc gccacggcga tggatgtgcc gtcctatgac
    17701 gatcttcagg aaatcgggag gcgctcaacg ctggtgctgc cgacttttgg aattcatcct
    17761 cgacgcgctc cgcaatatgc cgaccggctg cgcgaactcg gtcaatatat cgagacaagt
    17821 cccgccatcg gcgaaattgg gcttgatttc cactgggtca aggatccctc cgagta
//
LOCUS       DASUVS010000432         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1286158, whole genome shotgun sequence.
ACCESSION   DASUVS010000432 DASUVS010000000
VERSION     DASUVS010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286158"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..803)
                     /locus_tag="VF182_22760"
     CDS             complement(165..803)
                     /locus_tag="VF182_22760"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX6439957.1"
                     /translation="MNIAILIYDGLTALDAVGPYEVLSCLPGAKVHFVSTELGPKRAH
                     TNFLSVMADYTLNDISDPEIIVVPGGTKGTMVAAQDPRILSWLRKAHETSKWTTSVCT
                     GSLILGAAGILKGLQATTHWYARDVLEKFGAQYLSERVVRQGKIITAAGVSSGIDMAL
                     HLAQEVSGPEMAKTIQLIIEYDPKPPFDSGSIAKAEPSIVELAKHAAQDTFK"
     gene            1442..1996
                     /locus_tag="VF182_22765"
     CDS             1442..1996
                     /locus_tag="VF182_22765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX6439958.1"
                     /translation="MAKWFFEPGHTAAEFSVRHMMVTLVRGHFKDIHGTLVFDPVNLG
                     ATSVDATIEAAGIWTGEAARDAHLKSEDFLDVEKHPKITFAGKQIELAGAAEAVLTGE
                     LTIRGITRTVPLRVHYLGQWQTPWWEDGVDKGPKSRAGFAATTTINRHDFGVSWNSAL
                     DRGGVVVGNMVEITIDAEAILQEG"
     gene            2215..>2844
                     /locus_tag="VF182_22770"
     CDS             2215..>2844
                     /locus_tag="VF182_22770"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439959.1"
                     /translation="MKFTNVLVLLACVFANLLGVRDLYAQAERLRVAMASISTSQVNL
                     WVPRDVGLFKKHGLDVDLVLISGAPIVNAALLSGEVAIAQGGPAPSIQTNLKGAGTFI
                     ILGNTNRFPYQLVATPNTKDITDLKGRRFAIARLGAADHTAVLFVLPRLGLQPEKDLS
                     LIAVGAVPSRFAALANGAVAATLLIPPETSKARELGFRVLSNFMDIDVEF"
BASE COUNT          653 a          715 c          788 g          688 t
ORIGIN      
        1 acaagaaatg gtcgagaaac attttggtgt tcaaagccat cagcctaaac acggctcgga
       61 taaactcggt ggtaattttc gtttaacctg aactttacaa ttctgcttat caggctaaaa
      121 ggaaccggct tgtcccatgc aattgcaaaa gatgcgataa atgttcactt gaacgtatcc
      181 tgcgctgcat gctttgctaa ttccacaatt gaaggctccg cttttgcgat ggatccggaa
      241 tcgaagggtg gttttggatc gtattcgata atcaattgaa tggtcttcgc catctccggt
      301 cctgagacct cctgcgcaag gtgaagagcc atatcgatgc ccgaggaaac gccagccgcg
      361 gtgatgattt tgccttgccg aaccactcgc tcactcaggt actgagctcc gaacttctcc
      421 agaacatctc gggcatacca atgcgttgtt gcctgtagcc cttttaaaat ccctgccgcg
      481 ccgaggatta gtgatcccgt gcagacagag gtagtccact tcgaagtttc gtgtgctttt
      541 cgaagccagg agagtatgcg cggatcttgg gccgcgacca tcgtcccctt ggttcccccc
      601 ggcaccacaa tgatttcagg atccgagata tcattcagcg tgtaatccgc catgacagaa
      661 agaaagttcg tgtgcgctcg cttcggaccg agttcggtag aaacaaaatg gacctttgct
      721 cccggtaaac aactcaggac ttcataagga ccgaccgcat cgagcgcggt caaaccatcg
      781 tagatcagaa tcgcaatgtt catgaatcct cctaaataaa accgttctac tgtaacagat
      841 gatactcagg attggattgt cgaaaaaccg actgggcaaa attaaaacac agcgctgctc
      901 ttgggtggta gcgtcgatga tccgggagat cacgcgctcg agattgttgg cgagggaccg
      961 cgtcagtcat gatgctttgc tcggctttca acagagcgtg cattttttaa ttgcttcgat
     1021 actgattgtg ccgggcgacg agtttcggga tgcgcgacga gccccgtcgg gtggctggca
     1081 actcagaaat cgccatgacg cgctgcgagt ggcgtcgaga gccgcgccta cagctatcac
     1141 aaatcgcgag ggaatttgga attcattgta attactgtag gacgaatcgt gcgccagccg
     1201 atgaagcgag ttagcgtagc ccgcgggaga acgtagcgag tggtcgattc gggctgtgat
     1261 aacgtgacca ccctacgacc gcgctcgcga agtgagcggg ttaaaaagca attttacgcc
     1321 gggatatttc ctgttgcgaa tgccttttgc ctcgaagacc ggctgacgcg attgaatccg
     1381 ctaatccagg ggttaacgta aagttgattc cagggaatat cttacttcga aggagcggac
     1441 tatggccaaa tggttttttg aaccggggca tacggcggcg gagttttctg tgcgtcacat
     1501 gatggtgact ttggtccgcg gccatttcaa agatatccat ggcacgcttg ttttcgatcc
     1561 ggtgaacctc ggcgcgactt ccgtcgatgc cacgatcgaa gctgctggaa tctggacagg
     1621 cgaagcggct cgcgacgctc atcttaagag tgaggatttt ctcgacgtag agaaacatcc
     1681 gaagattact ttcgcaggaa agcagatcga gcttgcgggc gcggccgaag cggttctgac
     1741 tggcgagttg acgattcggg gtatcacccg cacggtgccg ctgcgcgtcc attatctagg
     1801 tcagtggcaa actccatggt gggaagatgg agtggataag ggaccgaaga gcagggcggg
     1861 ctttgccgcc acgacgacga tcaatcgcca cgacttcggc gtgagctgga acagcgcatt
     1921 agataggggt ggggttgtcg tcggcaacat ggttgagatc accatcgatg ccgaagcgat
     1981 cctgcaagaa ggttgatgct gcacagcttc atcggacccg gaaaccaatt tgctaccatc
     2041 gaagcttttc gtcaccgctc cggagtcggt cggaatttcc atgaatttta cgggatggcg
     2101 gatggtagac cccccatgtc ccatctgtga ccccccattt gtatgagtta ttgttactga
     2161 ttcgaggaga tctttatagt tgatattctt ggcctttcgg aatagttcca aaccatgaaa
     2221 ttcaccaatg ttttagtttt gcttgcttgt gttttcgcaa atttactcgg cgttcgtgac
     2281 ttgtatgcac aagcggaacg tcttcgcgtg gcgatggcgt cgatcagtac aagccaagtg
     2341 aatctttggg tgccccggga tgtcggtctg tttaaaaaac atgggctcga tgtcgatctc
     2401 gttttgatca gcggcgcgcc gatcgtcaac gccgcgcttt tgtccggcga agtcgccatt
     2461 gcccagggag gtccggcgcc gtcgatccaa accaatctca aaggcgctgg cacctttatc
     2521 attctgggga atactaatag atttccctac caactggtgg caacgcccaa tacaaaagac
     2581 atcacggatt tgaaaggcag gcgctttgcg atcgcgcgac tcggggccgc cgatcacacc
     2641 gcggttctgt tcgtgttgcc gaggctcggc ctgcagccgg aaaaggacct tagtctgatc
     2701 gcggtgggcg ccgtgccgag ccgtttcgcg gcattggcca acggcgctgt cgcggcaact
     2761 ctgttgatac cgcccgaaac cagcaaggca agagaattgg gatttcgtgt cctgtcgaac
     2821 ttcatggata tcgatgtcga gttc
//
LOCUS       DASUVS010000433         6965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_191953, whole genome shotgun sequence.
ACCESSION   DASUVS010000433 DASUVS010000000
VERSION     DASUVS010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191953"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="VF182_22775"
     CDS             <1..1193
                     /locus_tag="VF182_22775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6439960.1"
                     /translation="PQLAQARVGSKGEFIFDVQGHFVDPTGAWVKSAPADAFKWSPKT
                     SCGLSAKPGARSYLNCLGPEEFVKDVFLDSDTDMMVLSFVPSRRDSEPLTIQAADAVR
                     RTIERMEGSHRLLLHGRVNPNQPGDLEAMDELKERWKISAWKTYTQWGVDGKGFFLSD
                     EIGTRFIEKARALGVKVICVHKGLPFGKQSYEHSQCSDIGVVAKRFPDVSFLIYHSGF
                     VTGVSERAFNGRGAGDGIDTLIRSLVENEVKPNSNVYAELGSTWRFLMRDPDGAAHAL
                     GKLVKYCGENNVLWGTDSIWYGSPQDQIEAFRAFQIAPELRDKHGYAEITPALRAKIF
                     GLNASRIYAISAEDVKKHTRADTIGKERLAYREHPEPHYLTYGPKTRREFLNLLRWNG
                     GSRA"
     gene            complement(1374..2420)
                     /locus_tag="VF182_22780"
     CDS             complement(1374..2420)
                     /locus_tag="VF182_22780"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6439961.1"
                     /translation="MRVSIIVLSFILMLAASASAQGNKPSTLAELAKYNGADRERILY
                     EGARKEGKLLWYTSLVPNKHIAKVFQAKYPGVAVDVYRAAGNTLATRLLEERRAKRYL
                     ADAIESTPPQLMLLRDAQALQPFLSPHLANYPEVAKESASGDLVFWATDRESLIGVGY
                     NTNVMPAALVPKKFDDLLNPALKGKMSTANNETGGRAIGAMLKAKGEAFVRKLRSQDI
                     RPHTTTAAGLTDLIISGEAPLSFTMVQTNLTQPAATKGAPVAWVPMEVVAVNAGSAAV
                     IANAPNPHAALLFVDFLLGPDGQKMYSEQLFYGSAAKVSGFERWYPEKGLTTEDYEKR
                     ADGWLQLLQEITRN"
     gene            complement(2538..3353)
                     /locus_tag="VF182_22785"
     CDS             complement(2538..3353)
                     /locus_tag="VF182_22785"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6439962.1"
                     /translation="MATYETLLVSTENGITTVTFNRPEKRNAMSPQLHREMFDLLTEI
                     RYDKETRVVVLTGAGDNFCAGQDLKQYSLEMESQPEQVRDEVREKVRRWRNEMLRTLP
                     QPVIARITGWCLGGALTVVAGCDIALAAEDALFGLPEVNFGHFPAGDTTSVMTEHLQP
                     KHGLYYALTGKMMTAREAERIGLISRAVSKSELDREVAELALSLREKSPLGLKAVKEA
                     WYYSSYAAPDVAYEISNLISQRTIRDHGGRPGLEQFVQKKLRPTSGVMQLNEK"
     gene            complement(3718..5100)
                     /locus_tag="VF182_22790"
     CDS             complement(3718..5100)
                     /locus_tag="VF182_22790"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX6439963.1"
                     /translation="MAFSSISIRKTSDRKHRRLADSALLLLLVWISSCSPALQLNQGS
                     AQSVSKKVSFAILEDYDKGEDLNEIAKDFQLMRELEIDTLRCSFGWDDFEPVRGQYDF
                     AWLKEFVKLAAQHGIKLRPYIGYTPRWAGTAGSADGADWNNPPADYQAWHDFVYNLVS
                     ALREYPNVLSYEIYNEENSPQWWDGSIDQYKETLRHGALAIRAANPAAQIILGGFVFP
                     DVEWLQAIAGDGYARYYEITAFHAYPETWTGSDVVVENYLDRHYRESFVVDNRTFGEA
                     EPIWINEMGFSVTPDKTEREQANWWARAVSTFLADPHVEHIGVYEIKDLPLGKETIGD
                     QKNHYLGITRADRGKKLAFYTVDLLTDLLDVGTLTTAPNELTVAVSAGEAKELYHHLF
                     KRPDGSQVLFIYDKSASPTVSATLKTQGRTAVKYELDGSSEKHTGFDGHTLTDIRLTA
                     GNVAIFRIDP"
     gene            complement(5265..6272)
                     /locus_tag="VF182_22795"
     CDS             complement(5265..6272)
                     /locus_tag="VF182_22795"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6439964.1"
                     /translation="MAAAEFIVDVHHHFMPAVLFDRLAAQAGGKRIVTNEISLTLNPS
                     RKDLEAHLKTMDDSGVTVSILTDQVQVMGADVARALNDGIAEVATKHPTRFRGAIHLP
                     IHEPEAAKRELERGINELGLRAVALLACHLDIQLDNPIMNPLFEIIQKNNLPIIIHPQ
                     SKPTGSETTYNLDRCVFRPLETTQAIVRVMCSVLPRYPELRFIMPHLGGATSSLKGRM
                     MAFFETDDADIPADMRGYLKTQNEQKKFGITERFEKLFQSLYFDTAGTGAWLPAMAAA
                     LNTTTADRIMFGSDYPLECKSAANIIESLDMIRQAPCSTTEKTAILGKTAAGLFNLKT
                     S"
     gene            complement(6363..>6965)
                     /locus_tag="VF182_22800"
     CDS             complement(6363..>6965)
                     /locus_tag="VF182_22800"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX6439965.1"
                     /translation="GGGEVGSKVVELLTDAGEETLLIDNQPRPGVDILGNVLDTQILK
                     QANIQDAQAVIVALSADTTTLFTTVIIKDLAPDVPVIARVNRAENVERIYAAGADFAL
                     SISQVSGQLLAWRLLGKETVDIDADLRVVKVSVRGMAGRHPDELGIREKTDCSAIAVE
                     RGNDLLVEFGQGFRFQDDDALYICGSAEATQKYAETFPQN"
BASE COUNT         1407 a         1969 c         2008 g         1581 t
ORIGIN      
        1 agccgcagct ggcgcaggcg cgagtcggga gcaaaggcga gttcatcttc gatgtgcagg
       61 gtcacttcgt tgaccctacc ggggcatggg tgaagtccgc gcccgccgat gctttcaaat
      121 ggtcgcccaa aacaagctgc ggcctgtctg ccaagcccgg agcgcgcagt tatctcaact
      181 gcctcggacc cgaggagttc gtcaaagacg tgtttctcga cagcgatacc gacatgatgg
      241 tgctctcgtt tgtgccgtcg cgccgtgact cggagccgct gacgatccag gccgccgatg
      301 cggtgcgccg taccatcgaa cggatggaag gctcgcaccg gctcttgctc catggacggg
      361 tcaatccgaa tcagcccggc gatctcgagg cgatggatga attgaaggag cgctggaaaa
      421 tcagcgcctg gaagacctac acgcagtggg gcgtggacgg caagggattt ttcctatccg
      481 atgagatcgg cacccgcttc atcgagaaag cgcgggcgct cggcgtgaag gtgatctgcg
      541 tgcacaaggg tctgccgttc ggcaagcagt cctacgagca cagccaatgc agcgacatcg
      601 gcgtggtcgc caaacgtttt ccggatgtaa gttttctcat ctaccattcg ggttttgtta
      661 ccggagtttc cgagcgcgcc ttcaatggcc gcggcgccgg cgacggtatc gacaccctca
      721 tccgctcgct agtcgaaaac gaagtcaaac ccaacagcaa cgtttacgcc gagctcggca
      781 gcacctggcg ctttctcatg cgcgacccgg acggcgcggc ccatgcgctg ggcaagctcg
      841 tcaaatattg cggcgagaac aacgtcttgt ggggcaccga ttcgatttgg tacggctcgc
      901 cccaggatca gatcgaagcg tttcgcgctt ttcagatagc gccggaactg cgcgacaagc
      961 acggctatgc cgagatcaca ccggcgctgc gcgccaagat tttcgggctg aacgcgagcc
     1021 ggatctacgc catcagcgcg gaggacgtga agaagcacac ccgcgccgac acgatcggca
     1081 aggaacgcct ggcgtaccgc gagcatcccg agccgcacta tctcacctac gggcccaaga
     1141 cccggcgcga gtttctaaac ttgcttcgct ggaacggcgg ctcgcgcgct tagcaggctg
     1201 ctcaaaacga cccatatgcc ttgggaaagg atgaattatg aaagctgaaa actgaattcc
     1261 gctttcagat ttccgctttc atccttgtgc ttcgcgcgcc tcgtctctgg actttttgcg
     1321 cagcctgcaa cctttctcgt aacgagtcgg cgaaataggg gaacagcgct gcgttagttg
     1381 cgcgtgatct cctgcaagag ctgcagccaa ccgtcagcgc gcttttcgta atcttcggtc
     1441 gtcaaaccct tttccggata ccagcgctcg aatcccgaaa ccttcgcggc gctgccgtag
     1501 aagagttgct ccgagtacat cttctgtcca tccggcccga gcaagaaatc gacaaacaac
     1561 aaagccgcat gaggattggg cgcattggcg atgaccgcgg cgctgcccgc gttgacggcc
     1621 accacttcca tgggcaccca ggcaaccggc gctcctttgg tggcggcggg ctgcgtcaga
     1681 ttggtttgga ccatcgtgaa cgacaacggc gcctcgccgg agatgatcag atcggtcagc
     1741 cccgcggccg tggtcgtatg gggccggata tcctgtgatc gcaactttct aacgaaggct
     1801 tcgcccttgg ccttgagcat cgcgccaatc gcccgccccc cggtttcgtt gtttgccgtt
     1861 gacatcttgc ctttcagtgc cggattgagc aggtcgtcga atttcttcgg caccaacgct
     1921 gcgggcatga cgttggtgtt gtaaccgacg ccgattagag attcccgatc ggttgcccaa
     1981 aacactaaat cgcccgatgc ggattccttg gcgacttccg gatagtttgc caggtgcggc
     2041 gacaagaacg gctgaagcgc ctgggcatcg cgcaacagca tgagctgagg cggggtcgat
     2101 tcgatcgcat ccgccaggta tcgtttggcg cggcgttctt ccaacaatcg ggtagccagc
     2161 gtattgccgg cggcgcggta aacatcgacg gcgacaccgg ggtatttggc ttgaaacact
     2221 ttggcaatgt gcttattcgg taccagggac gtataccaaa gtaatttgcc ctcttttcta
     2281 gcgccttcgt aaagaatccg ctcgcggtcc gcgccgttgt atttcgccaa ctcggcgagc
     2341 gtgctcggtt tgttgccctg cgccgatgcg ctcgccgcca gcatcaagat aaaagaaaga
     2401 actataatag aaactctcat cgctcttttg ctcctttgtc tgctttggta acacggccgg
     2461 ccgttgatca acaagatcga tctggttcat ccattgttat tggcgccgtt tgctttcaaa
     2521 tctgaatact cgttcgatca tttttcgttt agctgcatca cgcccgatgt cggtctgagc
     2581 tttttctgga caaactgctc taggccgggc cggccgccgt gatcgcgtat ggttcgctgc
     2641 gaaatcaagt tggaaatttc ataggcaacg tcgggcgccg cataggaact gtagtaccac
     2701 gcctctttga ccgcttttaa acccagcgga cttttttccc tgagggataa agcgagctcg
     2761 gcaacctctc ggtccaattc cgacttagaa acggccctcg agatgagccc gatccgttcg
     2821 gcctctcggg cggtcatcat cttgcccgtg agcgcgtagt agagcccgtg cttgggctga
     2881 agatgctcgg tcatcaccga ggtcgtatct ccggcaggaa aatggccgaa attgacttcc
     2941 ggtaaaccga acagcgcatc ttcggcggcc agcgcgatat cgcagccggc caccacggtg
     3001 agcgctccgc ccaggcacca gccggtgatg cgcgcgatga ccggctgcgg tagagtgcgc
     3061 agcatttcgt tgcgccagcg gcgcactttt tcccggactt cgtcgcgcac ctgctccggc
     3121 tggctttcca tctccagaga atactgcttc aggtcctgcc cggcgcaaaa gttgtcgccg
     3181 gctcctgtga ggacaaccac acgggtctct ttgtcgtagc gaatctccgt cagcaggtcg
     3241 aacatctcac gatgcaattg gggactcatc gcgttgcgct tttccgggcg gttaaacgta
     3301 accgtcgtga tgccgttctc cgtcgatacc agcagggttt cataggtcgc cattttcttt
     3361 tctcctttag tgtagattca aaccgaatgt atcgagaggt gattccgagc gccacctctt
     3421 cgtcgcagct tgtcgctcgt tctcagaccg gaaaaatgtt tattgaaagc ccggcaacac
     3481 taagctcagg tcccttgttg ccgcgtcgag cgagatgctc gcggcttagc aacagccagg
     3541 tcgacgccca ggattgctta cagtgtgctg aaaaagcgtc ttgcaggctg ctcaaaaaaa
     3601 tccagaggtg aggcacgcga agcacaagga tgaaagcgga aatctgaaag cggaattcag
     3661 ttttcagctt tcataattca tccttcccca ggcatatagg tgtttttcag cagcctgtta
     3721 gggatctatg cgaaagatgg cgacgttccc ggccgtcagg cgaatgtctg tgagagtatg
     3781 gccgtcgaat ccggtatgct tttcggagct cccatccaat tcatatttca cggcggttct
     3841 tccttgcgtc ttcaaggttg cgctgacggt tgggcttgcc gatttatcat agatgaacag
     3901 gacctggctg ccgtccggtc ttttgaaaag atggtggtac aactctttgg cttcacctgc
     3961 cgacaccgcc accgtcaact cgtttggggc ggtggtaaga gtgccgacgt ccaacagatc
     4021 ggtcagcaga tcgacggtgt agaaggcgag ttttttgcct cggtcggctc gagtgattcc
     4081 cagataatga tttttctgat cgccgatggt ctccttgccg agcggcagat ctttgatctc
     4141 atagacgccg atgtgttcta cgtgaggatc ggcaagaaag gtgctgaccg cccgcgccca
     4201 ccagttggct tgttcccgtt cggttttatc cggcgtaacg gaaaaaccca tctcattgat
     4261 ccagatcggc tccgcttcgc cgaaagttct attgtcgacc acgaaagact cgcggtaatg
     4321 gcggtccaaa taattttcga cgacgacgtc actgccggtc caggtctcgg gataggcatg
     4381 gaacgcagtt atttcgtagt aacgagcgta tccgtcgccg gcgattgcct gaagccactc
     4441 gacatcggga aacacaaacc cgcctagaat gatttgcgct gccgggttgg cggcccggat
     4501 cgccagcgcg ccgtggcgca gcgtctcttt gtattgatcg atggagccat cccaccactg
     4561 cggcgagttt tcctcgttat agatttcgta cgaaaggacg ttgggatatt cacgcaaggc
     4621 ggagacaaga ttgtaaacga aatcgtgcca ggcttgataa tcggcgggag gattgttcca
     4681 gtcggcgcca tcggcgctgc cggcagtgcc cgcccaacgc ggcgtgtagc cgatgtaggg
     4741 ccggagcttg atgccatgct gcgcggcgag cttcacgaat tctttcaacc aggcgaaatc
     4801 gtactggccg cgcaccggct caaaatcgtc ccagccgaag ctgcatcgca gcgtgtcgat
     4861 ttccaactcg cgcatgagtt gaaaatcctt ggcgatctcg ttcagatctt cgcctttgtc
     4921 gtaatcttcc aaaatcgcga aagagacttt tttgctgacg ctctgcgccg atccctggtt
     4981 gagttgaagc gccggactac aggacgagat ccagacgagg agcaggagca gggcagagtc
     5041 ggcgagcctt cgatgcttgc ggtccgacgt tttgcgaatt gagatcgagc taaacgccat
     5101 agctcctgtt ggttcgtctc cttagacggc ctgaacaccg cgggggcagg ttcaacttgg
     5161 cgagtggcgg tgaagaattt actcgatatg ttttccggcg ctgcttttgg aatcgcagag
     5221 ctaagtttcc ccagccgacc gtgcgcggct ccaggaaaca aaactcagga tgtctttaga
     5281 ttgaacaagc cggccgcggt tttgccgaga atcgcagtct tctccgttgt cgaacaggga
     5341 gcctggcgga tcatgtccaa ggactcgatg atatttgccg cgcttttgca ctccagcggg
     5401 taatcgctgc cgaacatgat gcgatcggcg gtggtggtat tgagcgcggc ggccatggcc
     5461 ggaagccagg cgccggtacc ggcggtgtcg aaataaagcg attgaaagag tttttcaaat
     5521 cgttcggtga tgccgaattt tttctgctcg ttttgggttt tcaggtagcc tctcatatcg
     5581 gctggaatat cggcgtcgtc ggtttcaaag aaagccatca tccgtccttt gagcgaggaa
     5641 gtggcgccgc ccaggtgagg catgatgaaa cgcagctcag gatagcgcgg cagcacgctg
     5701 cacatgaccc gcacgatggc ctgcgtggtc tcgagcgggc gaaacacgca gcgatcgaga
     5761 ttgtacgtcg tttccgagcc ggtgggcttg gactgcgggt gaatgatgat cggcagattg
     5821 tttttctgga tgatctcgaa gagcgggttc atgatcggat tgtcgagttg aatgtcgaga
     5881 tggcaggcga gcagcgccac cgcgcgcaat cccaactcgt tgatgccgcg ctcgagctcg
     5941 cgcttggccg cttcgggctc atgaatcggc aggtgaatcg cgccgcgaaa gcgagtcggg
     6001 tgtttcgtcg cgacctcggc gatgccgtcg ttcagagccc tggccacatc cgcgcccatt
     6061 acttgcacct gatcggtcag gatggacacg gtcacgccgg aatcgtccat ggttttaaga
     6121 tgggcttcga ggtccttgcg cgaaggattg agggtcaaac tgatctcgtt cgtcacgatc
     6181 cgtttgccgc cggcttgcgc cgccaggcgg tcaaagagca cagccggcat aaaatggtga
     6241 tgcacatcga cgataaattc tgctgcagcc atcttggatt tactcctttg cgcttgttta
     6301 acgaatgagc cagcgtacgt cactgccccg ttcggttcaa gcgatgtacg gggctggacc
     6361 gattagttct gaggaaacgt ttcggcgtat ttttgtgtgg cttcggcgct gccgcaaatg
     6421 tacaaggcgt catcgtcttg aaagcggaat ccctgaccaa actccaccag caggtcattg
     6481 ccgcgctcga ccgctatcgc tgagcaatcg gttttttctc gaatgccgag ctcatccgga
     6541 tgacggcctg ccatcccgcg caccgagacc ttgaccactc ttagatcggc gtcgatatcg
     6601 acggtctcct tgccgagcag acgccaagcc aacaattgcc ctgaaacctg gctgatggag
     6661 agcgcgaaat ccgcccccgc cgcataaatc cgctcaacgt tttccgcgcg attgacgcgc
     6721 gcgatgactg gtacgtcggg cgcaaggtct ttgatgatca cggttgtgaa aagagtcgtg
     6781 gtgtcggcgc tcaaggcaac gatcacggct tgcgcgtctt gtatattcgc ctgtttaaga
     6841 atttgggtat cgagtacgtt accaaggatg tcgacgccgg gcctcggctg attgtcaatg
     6901 aggagagtct cttcgccggc gtcggttaac agctcgacta ctttgctacc gacctcaccg
     6961 ccgcc
//
LOCUS       DASUVS010000434        12403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_630654, whole genome shotgun sequence.
ACCESSION   DASUVS010000434 DASUVS010000000
VERSION     DASUVS010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12403
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630654"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(419..1450)
                     /locus_tag="VF182_22805"
     CDS             complement(419..1450)
                     /locus_tag="VF182_22805"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6439966.1"
                     /translation="MRKLFKSCVLGIAVMAMLTLSPSMVAAQSQKLTVGWSAVSALNA
                     PYWVMKDAGFLNKEGLDANLVYIPSSSTIAQAQVSGNIDISTANSQVIVDADLRGGDL
                     VAIGAISNAVAFYVMANPAITSVTELKGRKVGVTRFGASTDFAVRKLLEKYHMEAISD
                     VPIVQIGGMPEIAAALSNGAIAAAPMSYPMAYVAQQNGMKLLANMAEEGIAFVHVGIT
                     TSRGFLGQRRSQAKAFLRAYARAVHYMHTNKEGFKKIITQYSKITDPGMLEGSVQYAY
                     DFVEKVPLVDRQAFRVTVDQIAEKRPRAKSAELDQFFDNSLVQELISEGFFKSLWGNQ
                     LNLEKFSNR"
     gene            1987..2412
                     /locus_tag="VF182_22810"
     CDS             1987..2412
                     /locus_tag="VF182_22810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439967.1"
                     /translation="MRLRTLAICATIVSISPALDSASAVTVLTSQQAVKALSFEKLDV
                     TPAKISGVIVNKTPHTIKDVQLMIQYHWLWTDERHPGQNSPGTTVIIPLNEQFEPGES
                     HSFSYTPQVALPSREDGAFMPEVDIAGFTTVVQQRPSAR"
     gene            2696..3106
                     /locus_tag="VF182_22815"
     CDS             2696..3106
                     /locus_tag="VF182_22815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439968.1"
                     /translation="MNRHDSAKLGKIMLVCSKPADEVEKCLRSVGGEIVTVNDGEAAI
                     TQAQRATFNLAVLVSTGKTMDLAETVFNLRDISASMRILIIDERHGARKSQADLIAAA
                     SPNTRTLTLGDLPAYLSVSSVHIPRRISGRRHQQ"
     gene            3338..3721
                     /locus_tag="VF182_22820"
     CDS             3338..3721
                     /locus_tag="VF182_22820"
                     /inference="COORDINATES: protein motif:HMM:NF028042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX6439969.1"
                     /translation="MHPRVSRSKTVIGAKGFSLVELLVVIAIIGLLAAIAIPQFVWYR
                     AGAVDSQMKSDLKNAALAMDSYFAENKEYPTSLAAIVGVGFNQTEGVALTIAVPTPSS
                     FTITATKPSGTQPSFTFNSVTGNIN"
     gene            complement(3776..4741)
                     /gene="corA"
                     /locus_tag="VF182_22825"
     CDS             complement(3776..4741)
                     /gene="corA"
                     /locus_tag="VF182_22825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX6439970.1"
                     /translation="MIINCAAYENGHKLADISVEEIPAYISRPACFVWIALKDPQTSE
                     LEKMQQVFGLHELAVEDAQHGHQRPKIEEYGESLFVVLHMIEFAGDELVVGETEIFVG
                     KQYVLSVRRQAQHGFVEVRQRCEREPDLMKHGTGYVLYALMDAVVDRYFPILDALETN
                     LERIEEQIFANQSSRANIEDLYELKRKLTVLKHATEPLIEAITRLFGGRVPQLCVSLQ
                     DYFRDVYDHLIRLNQSIDSLRDMVVTAISVNLSLISIQENEITKRLGSYAALITVPTL
                     IAGIYGMNFHNMPELDSPLGYPLALITMAVIDLYLFIRFRKAGWL"
     gene            complement(4905..5636)
                     /locus_tag="VF182_22830"
     CDS             complement(4905..5636)
                     /locus_tag="VF182_22830"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6439971.1"
                     /translation="MNEVIIVTGGAGGIGSDLCRGLAGDGLKVIIADYAKDAAEKVAA
                     EIRQSGGEASAIQVDVGNPQSVAAMMDQSIAKYGAVDYVFCGAGVMDRIAVVDMPEEE
                     WDRVMRINLKGVFLCSQAAARHMLPKKKGRILSIASGRGVAGQARAAHYAASKAGVIA
                     FTKSLAMELAPHDITVNAVCPGATDTPMSRIGFTAEQFKKREEVPPLLDGLTQKDEIV
                     GLVKYLLSDATRFVTGQTFFLRTPR"
     gene            complement(5973..6380)
                     /locus_tag="VF182_22835"
     CDS             complement(5973..6380)
                     /locus_tag="VF182_22835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEX6439972.1"
                     /translation="MRLVLLFIGLPLLELYILIKIGSYLGPFLTIMLIIFSALLGLLL
                     ARFEGLRTWHQIRQNLAQGILPAEEMLDSVLIFAGGVLFVIPGVITDVAALILLVPFT
                     RTIFKRWLRRRFDRMVERGNVRLQYHRSAPDDH"
     gene            complement(6383..7300)
                     /locus_tag="VF182_22840"
     CDS             complement(6383..7300)
                     /locus_tag="VF182_22840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX6439973.1"
                     /translation="MRICSLLPGATEIACALGLADEIVGVSHECDFPPAARSKPVMVR
                     SRIDPTKIGSGEIDRRVGDLLRSKQSLYTLDEKLFTDSQPDIILTQGLCDVCALDYND
                     VVQAARSLAREPRIISLDPHCLDDILNDVLRIGEATHRSLQAESLVQELRVRIEAVRH
                     QTARSASRPRVACLEWFDPLYIAGHWVPKMVQLAGGSDILGTAGEPSAKIEWGALTES
                     RPEILILMPCGFDLDRAVRESSLLKNRAGWQSLAAVRDKRVFAVSGADYFSRPGPRLV
                     DGLEILSQLFHPELFPVPISSDLAKRVNL"
     gene            7700..8425
                     /locus_tag="VF182_22845"
     CDS             7700..8425
                     /locus_tag="VF182_22845"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX6439974.1"
                     /translation="MSERIWDRLLSEQDRAHLAVSPRKHIGLGTKPALLLIDLYRWVF
                     GDKPEPLLEAIKTWPLSCGLAGWNALPHVQKLLAVAREIGIPVIHVVNMDGNGVEGRY
                     PNKGREDLSPEALERRRRKYEIVDELKPVPGETVLQKSSPSAFWGTPLIGHLNWLRVD
                     TILVCGESTSGCVRASVVDGCTNRFRMVVAEECVFDRHETAHAMNLFDMDRKYAAVLP
                     VDDVIKYLRGWQVEIGGQVKSVG"
     gene            complement(8435..9463)
                     /locus_tag="VF182_22850"
     CDS             complement(8435..9463)
                     /locus_tag="VF182_22850"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6439975.1"
                     /translation="MKMFYICLIALVLFISSSIGHAAAAETEKDRHDKLVEGAKKEGS
                     VVFYGSITASESETIRKVFESKYPFLKITQFRAGSDTLLQKVLTEARAGRREADVFNI
                     RSFEGNVLVERGLFAKYGSPHSKFYRDDFKDREGHWTSFYMNPATIGYNTKLVPRAEA
                     PKDWSDLLDPKWKGQMVMDREETEWFANMLKLMGRDKGLQYMRRLATQDLTFRAGHSL
                     MAQLVAAGEFKLGTVLYTPRMEVMKKSGAPIEWVRANPVIAYHYVVGVAAHAPHPNAA
                     RLFVDFLLSKEGQELLVKTSRVPVRSDVKAEPPTLVEGVNLMASDISLAPEYRKYYDE
                     YRQVFKVQ"
     gene            10012..10371
                     /locus_tag="VF182_22855"
     CDS             10012..10371
                     /locus_tag="VF182_22855"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX6439976.1"
                     /translation="MPRASRHFLPGYVWHITHRCHQREFLLKFARDRRRYLYWLFEAK
                     KRYGLSVLNYVAMSNHFHLMVKDTGANVIAASMQLIAGRTAQEYNQRKGRQHSGRIVS
                     RHGHRSRRAAASLSSVH"
     gene            10397..10810
                     /locus_tag="VF182_22860"
     CDS             10397..10810
                     /locus_tag="VF182_22860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439977.1"
                     /translation="MDDPEKWKESGFSEIQKPPKRYAIIDLQNLIELSGFADVRDYQT
                     AHRQWVEQGLENGLLMRDDRWTESIAVGSLAFIDQVKNELGFKADHRDVIEYDGSCVL
                     REAAEGYACKFAVENEALKSQNTFFWNEIVDEATI"
     gene            11053..11598
                     /locus_tag="VF182_22865"
     CDS             11053..11598
                     /locus_tag="VF182_22865"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6439978.1"
                     /translation="MGQLPSLTREQLSPHDRTVWDHVYSGRTLPPGGGPYAVLMHSPQ
                     MAQQFSNVENYYRNSGQLDPVDKELIILTIARAMNVRYPWGRHEIRARQVGMRSEALE
                     TLRANAPLDALNDHERLIVEMTRALLHERKLPEEMFARARAELGDQRLVEAVGLVGHY
                     NFISMVARTFDLDVPPGTMTF"
     gene            11762..12112
                     /locus_tag="VF182_22870"
     CDS             11762..12112
                     /locus_tag="VF182_22870"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX6439979.1"
                     /translation="MSCVFEAKKRFGLSVPNYMVTSNHVHLLVKDTGGDVIAQSMQLI
                     AGRSGQEYNRRKGHHGAAFWEDRYHATAVAVDEHLHRCLVFIDLNMVRAGVVKHPLSW
                     VYSGVCEVEQWLTR"
     gene            12193..>12403
                     /locus_tag="VF182_22875"
     CDS             12193..>12403
                     /locus_tag="VF182_22875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439980.1"
                     /translation="MDQALQGALPMRRAVDGSTCGWQSAFVEKVKEKLGPKALHRELD
                     RVDGTYALRESGEAYSSHFTVENEPL"
BASE COUNT         2891 a         3336 c         3331 g         2845 t
ORIGIN      
        1 cgacttttga gcagcgcttc gacctcggcc ttattttcga tgatgagaaa tcttgggacg
       61 gtcccggtat cgggcttggc ggaattcgaa ttggcaaacg atgatggagc aggcttcatg
      121 cgatcacgtg ttgatccggc ggtagcggtt tttcacggag cagctggcat gctcctgttt
      181 gttcgaaacc acagggcgaa acgaaatgct ttcggtgcac cccttgaggc tatactacaa
      241 agctaacgga tatcctaagg aaggcaaccc cgaatggatg cgtaggaccg gcctggtcaa
      301 ttggcttggt caagggccgg caatctattt ggcataaatc gccggccggg gatagacgcg
      361 ctacccgtgt aaagtttggg gctgggccgg tggagcggac gcgcagcaag acaccgcttt
      421 aacggttgga gaatttttcg agattaagct ggttacccca gagtgattta aagaagccct
      481 cgctgatcag ttcctgaacc agactgttat caaagaattg atcaagctcg gcgctcttgg
      541 cccgaggcct tttctcggca atctgatcaa ccgtgacccg gaacgcttgc cgatcgacta
      601 gaggaacctt ttccacgaaa tcgtaagcat attgcacgct gccttcgagc atcccaggat
      661 cggtgatctt ggagtactgc gtaatgattt tcttgaatcc ttctttattc gtatgcatgt
      721 aatgcacggc tctggcgtaa gcgcgcagaa aggccttcgc ttgactccgg cgctgtccca
      781 agaagccgcg cgaagtagtg atgccgacgt ggacaaaagc gatgccctcc tcggccatgt
      841 tcgccagaag tttcatgccg ttttgctggg caacatacgc catcggataa gacatcggcg
      901 cagcggcgat agcgccattg gaaagagccg cggcaatttc cggcattccg ccaatctgca
      961 caatcggcac gtcagatatc gcttccatat ggtatttttc gagcagcttg cgcacggcaa
     1021 aatcagtcga agcgccgaag cgggtaacgc cgactttccg gcccttcaat tccgtaacgc
     1081 tggtgattgc cgggttggcc atcacgtaaa atgcaacggc attgctgatg gcgccaatcg
     1141 ccaccaaatc gcctcctctg agatcggcat caacgatcac ttgactgttc gccgtcgaaa
     1201 tatcgatatt gcccgacact tgcgcttgag cgatggtcga tgagctcggg atgtagacca
     1261 gattggcatc caggccctct ttattcagaa accccgcatc tttcataacc cagtatggcg
     1321 cgttgagagc gcttaccgct gaccagccga ccgtcagttt ttgtgactgt gccgcaacca
     1381 tggaaggcga tagcgtcagc atcgccatga ccgcgatccc caacacacac gacttgaaga
     1441 gcttgcgcat cttaacctcg cttccgattg gcgcgtactg cggtaaattt ttgaagtccg
     1501 taatggactt caaacttggt tgtgcaactc gagtgccatg actgcagaga tccacgagcg
     1561 atcgaaggct caagccggcg agtctgtgca tgcgcaatca acgactgaca cgcgtccgga
     1621 aagctcattc aacgatcgac tggaatactc ttacgagtca tgatgagtga tccgacgacg
     1681 ctgccggaga gtttcgcagc tctcgacgag tgaagcgggt gtatacaatc gacgcatgtc
     1741 atctctcaga tgtgattgtt atgcacatag tccatgaaac tggcagccgc caaggattga
     1801 acggatgacg actcctggca gtttgttcgc tggcatgttg attgcgccct cgaaaaaatg
     1861 tcggagccca ccgcgagcga agcctgccgc gctgcgggtc taccgtagcg cctgacaggc
     1921 atcggctttc gcgggctcct gctgtgccga ttcgaagatg aagctcaatg gagggaggaa
     1981 cgtattatga ggcttcgtac gttggccatt tgcgccacaa tcgtttccat ttcgcccgcc
     2041 ctggattctg caagcgcagt gacggtgttg accagtcaac aagcggtcaa ggcgctcagc
     2101 ttcgagaagc tcgatgtaac cccggcgaag atttcgggag tgattgtcaa taagacgccg
     2161 catacgatca aggacgtcca gctcatgatt caataccact ggctgtggac agatgaacgg
     2221 catcccgggc aaaattcgcc cggcacgacg gttatcattc cgttaaatga gcaatttgag
     2281 ccgggtgaat cgcactcgtt cagctacaca ccgcaagttg cactaccgtc gcgagaggat
     2341 ggcgccttca tgccggaagt agacatcgcc ggtttcacca cagttgtcca acaacgaccc
     2401 tcagcccgtt gagttggaca acatgcaagc agcaattctc tgctactgaa gagggcgagt
     2461 tcactcatgg actttagccg gtccgccatc gttatagcgg cgcgccgggt tagattttct
     2521 cgcaaaaacc tctcacgggt cgggcggttc tcgtaaataa tcttcgttaa gaacactatc
     2581 cactgccgtt gtcaccccgg ggttagatcg tgccaaacaa aacgcttggt atttagattg
     2641 ctgccactct gaagtcgtaa agctccagag catgcgcgag caggaattgt aatcgatgaa
     2701 ccgacacgat tccgcgaaat tgggcaagat catgctggtt tgttccaagc cggcagatga
     2761 ggtggaaaaa tgcctgcgta gcgttggcgg cgagatagtc accgtcaacg atggcgaggc
     2821 cgcaatcact caggcgcagc gcgctacctt caacttggca gtgctggtct cgacaggtaa
     2881 aaccatggat ctggccgaga cagtttttaa tctcagagat atcagcgcat cgatgcggat
     2941 tcttattatc gacgagcggc atggcgcccg aaaatcgcag gcagatttga ttgctgccgc
     3001 ttcgcccaat acacggaccc tgactctagg tgacctgcct gcttatctga gcgttagcag
     3061 cgtacatatc ccgcggcgaa tttcgggcag gaggcatcaa cagtagagtg aaaattagcg
     3121 aaggatccta ggatctgtgt aaagttcatg cacatcttta ctttgaagat cttactctct
     3181 gcgttcttcc tctccaaatc atcgagttta tcaaccgaat ccgcactggc cttacccctg
     3241 ctccgggggg ctttacacgc caaataattg gtcttcctgg taagttgtgt gttaaactgc
     3301 cgcgtattta cttaaggcgg ctaacgctcc gactgctatg cacccgcgag tctctcgatc
     3361 taaaaccgtt atcggcgcca aaggctttag cctggtggag ctgctggtgg tcatcgccat
     3421 catcggtctg ttggcggcca ttgcgatccc gcaatttgtc tggtaccggg cgggcgcggt
     3481 ggatagccag atgaaaagcg atctcaagaa tgccgctctg gcaatggact cctactttgc
     3541 cgaaaacaaa gaatacccga catcgcttgc tgctatcgtc ggcgtcggct tcaatcaaac
     3601 cgaaggcgta gctctgacga ttgccgttcc cacgccgtct tcctttacaa tcactgcaac
     3661 taagcctagc ggaacccagc ctagtttcac tttcaacagt gtcaccggca acatcaactg
     3721 accctgctta gctggtttgt cgagatttct cacgaccacg actaatcgag ccccgctata
     3781 accaaccggc tttgcggaaa cggatgaaaa ggtaaaggtc gatcacggcc atggtgatca
     3841 gggccaacgg ataacccaac ggggaatcca gttccggcat gttgtgaaaa ttcatcccgt
     3901 agattccggc tatcaaggtc ggcacggtaa tcagcgccgc ataggagccc aaacgtttgg
     3961 taatctcatt ttcctggatt gagatgagcg acaagttgac cgagatcgcc gtgaccacca
     4021 tatcgcgcaa gctgtcgatg gactgattta agcgaatcag gtgatcgtaa acgtcgcgga
     4081 agtaatcctg caaactcaca catagttgcg gcacacggcc accgaacagc cgggtgatcg
     4141 cttcaatcag cggctcggtg gcatgtttca aaaccgttag cttgcgcttg agctcgtaga
     4201 ggtcctcgat attggcgcgg ctactttggt tagcgaagat ttgctcttcg atccgctcca
     4261 gattcgtctc cagggcatcc agaatcggga aatagcggtc taccaccgcg tccatcagcg
     4321 cgtagagcac atatccggtg ccgtgtttca ttagatcagg ctcgcgttcg caccgctggc
     4381 ggacctcgac aaagccgtgc tgtgcctgcc ggcgaaccga taagacgtat tgcttgccga
     4441 cgaagatctc tgtttcgccg acgacgagtt catccccggc aaactcgatc atgtgcagaa
     4501 cgacaaaaag cgactcgccg tactcctcga ttttgggccg ctgatgaccg tgttgcgcgt
     4561 cttcaacggc aagctcgtgc agtccaaaga cttgctgcat cttttcaagc tcgctcgtct
     4621 ggggatcttt caaggcgatc cagacaaagc acgccggccg gctgatatag gcgggtatct
     4681 cttcgacaga aatgtcggcg agcttatggc cgttctcata ggctgcgcag ttgatgatca
     4741 tagtgtctgg tttgctagtg ctctagagga cttgtgttgg ttcccgcggt gagagccaac
     4801 tgcgtgcttg actgcccacg gtttccggtc tcgccatcct cgctcactgc tgacaacgct
     4861 cgactattgt cggattaggg ccaagaaggt ccttgccgct gttttcacct gggagtgcgc
     4921 aagaaaaatg tctgtcctgt gacgaacctg gtcgcatcgc tcagcagata cttaaccagt
     4981 ccgacgatct cgtccttctg cgtgagcccg tccaggagcg ggggaacttc ttcgcgtttt
     5041 ttgaactgtt ccgctgtgaa accgattctc gacatcggag tatcggttgc tcccgggcac
     5101 accgcgttga cggtgatgtc gtgtggcgcc aattccatcg ctaatgattt ggtaaaggcg
     5161 atgacgccgg ctttggatgc cgcgtaatgc gcggctcgag cttgtccggc gacgccgcgc
     5221 ccggaagcga tgctcaagat gcgccctttt ttcttgggta gcatgtgcct ggcagcggct
     5281 tgcgaacaga gaaaaactcc cttgagattg attctcatga cccggtccca ttcttcttca
     5341 ggcatgtcga cgaccgcaat gcgatccatc acgccggcgc cgcagaaaac ataatcgacc
     5401 gcgccgtatt tggcgatgga ttgatccatc atggcggcga cgctctgcgg attgccgaca
     5461 tcgacctgga tggcgctcgc ttctccgcct gactggcgaa tttccgccgc gaccttttcc
     5521 gctgcgtctt tggcgtagtc ggcgataatc actttcaagc catcgccggc aagcccgcgg
     5581 cacaagtccg agccgatgcc gccggcgccg ccggttacga taatcacttc gttcatgtcg
     5641 tccttctcct gttcgaatcg ttgtgcggaa gctaccactt gtgcttacga cgggtcaatc
     5701 aggcttgata atttccgatc taaagagtgc tgatcttcgc tgcgccagcg gcttttcgcg
     5761 gttggcgagc ggaaacacga caagtgcaca ccgctcattg tgaaacggcc gcgaccgggc
     5821 gacagtttcc taaagaatct gactgcaggc tggtcaaaag atttcagaag ccaggcgcgc
     5881 gaatcacaag gatgaaagcg gaaatctgaa agcggaattc agctttcata attcatcttt
     5941 tcaaaagcat atgaggcttt ttcagcagcc tgttagtgat cgtctggagc gctgcggtga
     6001 tactgaagcc ggacgttgcc gcgctcgacc atgcggtcga agcgccgccg cagccagcgc
     6061 ttgaaaatgg tccgggtgaa agggaccaac aatatcaaag cggcgacatc cgtgatcacg
     6121 ccgggtatga caaaaagaac gcccccggca aagatcaaga cgctgtcgag catctcttcc
     6181 gccggcagaa taccttgcgc aaggttttgc ctgatctgat gccaggttct gaggccctcg
     6241 aagcgggcaa gcaacaatcc caagagcgcg ctgaagatta tcagcatgat cgttagaaat
     6301 gggccgagat agctgccgat tttaatcaga atatacaatt ccagaagcgg cagtccgata
     6361 aaaagaagaa cgagacgcat gcctatagat taactcgttt agctagatcg gacgatatcg
     6421 gaaccggaaa tagttccgga tggaaaagct ggctgaggat ttccaaacca tcgacgagac
     6481 gcggccctgg tcggctaaaa taatcggcgc cgctgacggc gaaaacgcgt ttatctctga
     6541 ccgcggctaa gctttgccag cccgcgcgat tcttcagcaa cgaactttct cgaaccgcgc
     6601 ggtccaggtc aaagccgcat ggcattagaa tcaaaatctc cggacgggat tcggtgaggg
     6661 cgccccattc gattttggcc gaaggctcgc ctgcagttcc cagaatatcg cttccgccgg
     6721 cgagctgaac catctttggc acccaatggc cggctatata gagcggatcg aaccactcca
     6781 gacaggccac tcgcggtcgt gaagccgatc gcgccgtttg atggcgtacc gcttctattc
     6841 gaactctgag ctcttgaacc aacgactcgg cttgcaaaga tcgatgggtc gcctcgccga
     6901 tacgcaagac atcgttcaga atatcgtcga gacaatgagg gtcgagggag ataatgcgtg
     6961 gctctcgcgc aagcgaccga gccgcttgga ccacgtcgtt gtaatccagc gcgcacacgt
     7021 cacataaacc ttgggtgaga atgatgtcgg gttgactgtc tgtgaataat ttctcatcga
     7081 gcgtgtaaag actttgcttc gagcgcagca gatcgcccac tcgtcgatcg atttcgccgc
     7141 tgccgatctt ggtcggatcg atccggcttc ttaccatcac cggtttgctt cgagctgccg
     7201 gcgggaaatc gcattcgtga ctgacgccga cgatctcgtc agccaatccg agagcgcacg
     7261 cgatctcggt ggctccagga agcaaggaac agatgcgcat tgcttgtgct tagcctatcg
     7321 atagcgcatt tgccacctga gccacgtgtg tcgcagccac tcggtcgcgc tacggatgtt
     7381 gacgcgattc gctgcgactt aatcaatttc aagcctcaaa acttcctttg ccgcggtcgt
     7441 tcttcgaaag agatcggtcc gaaaattccc aagtgggtag ttgcaccgga caagtcaata
     7501 actcacgccc gatcctcatg gaagtgccct agcatattcc aatgaacaca tctccgatgt
     7561 gattctcgga aattaaattc atcctcaaac gccatcacct aaactcactc tgatcataat
     7621 tgcccaggca ttcccttgac aatacgtagg agtcggaatg aaaatcactg cacgtaaagg
     7681 acattcgata ggagatcata tgagcgagcg aatttgggat cgactactga gcgagcaaga
     7741 ccgggcgcat ttggcggtaa gcccgcgcaa gcatattgga ttgggcacga agcccgcgct
     7801 acttctcatc gatctttacc gctgggtctt cggcgacaag ccggaaccat tgctcgaagc
     7861 gatcaaaaca tggcccttga gctgcgggct cgcgggttgg aacgcccttc cgcatgttca
     7921 gaaattattg gcagtcgctc gggagattgg cattccggta atccatgtcg tgaacatgga
     7981 cgggaacgga gtggagggca gatatccgaa taagggaaga gaagatttga gccccgaagc
     8041 gctcgagcgc cgaaggagga agtacgaaat tgtcgacgag ctaaaacctg ttcccggtga
     8101 aactgtcttg caaaaatctt ccccgagcgc tttttggggc acacctctaa ttggtcatct
     8161 caactggctg agagtcgaca cgatcctggt ttgcggcgaa agcacgagcg gctgcgttcg
     8221 cgccagcgta gtcgatggct gcactaatcg ttttcgcatg gtcgtggcag aggaatgcgt
     8281 cttcgaccgg cacgaaacag cacacgcaat gaatttgttc gatatggacc gaaagtacgc
     8341 cgccgtactg cctgtcgacg atgtcatcaa gtatctccgc gggtggcaag tggaaatagg
     8401 cggtcaggtt aaatcggtag gctagccgta ccggtcactg caccttaaac acctgccgat
     8461 attcgtcgta gtatttccgg tactccgggg ccaagctgat atcgctcgcc atcagattga
     8521 ctccttcgac aagcgtgggc ggttcggcct ttacatccga ccgcaccggt acccgagagg
     8581 ttttgaccaa taattcctga ccctccttgg aaagcaagaa atcgacgaaa agcctggcgg
     8641 cattcggatg gggtgcatga gcggcaacac caacaacata gtgatacgcg atcacgggat
     8701 ttgcccttac ccattcaatc ggtgcgccgc tcttcttcat gacttccatc ctgggagtat
     8761 acaatacggt gccgagcttg aactcgcccg cagccaccag ctgcgccatc aacgaatggc
     8821 ccgcgcgaaa agtaagatcc tgagtagcta gtcggcgcat atactgtagg cccttgtcgc
     8881 gccccattaa tttcaacatg ttggcaaacc attccgtctc ttctcgatcc atgaccatct
     8941 gtcccttcca cttcggatcc aagagatcgg accaatcctt cggggcttcg gcgcgaggaa
     9001 ccagcttcgt gttgtatcct atggttgcag gattcatgta aaaacttgtc cagtgtcctt
     9061 cccggtcctt gaaatcgtcg cgatagaact tcgaatgagg cgaaccatac ttcgcgaata
     9121 agcctctctc cacaagcacg ttgccctcaa aagaccggat attgaaaaca tcagcctcgc
     9181 gtcttcccgc ccgcgcctcc gtgagaactt tctgcagcag agtgtccgat ccagcacgaa
     9241 actgggttat cttcaagaac ggatacttgc tttcaaatac ttttctaatg gtctccgact
     9301 cacttgcggt aatcgaccca taaaatacca ccgacccttc cttctttgcc ccctcgacga
     9361 gcttatcatg cctgtccttt tcagtctccg cagccgcagc atggccaatc gaggacgaaa
     9421 tgaacagaac caaggcaatc aagcaaatgt aaaacatttt cattctttgc acctctgttg
     9481 tcgaactgag agcgccagta cagggcaatt ttcgcacgga gtcttagaga tatttgagga
     9541 tgtacccgac tgtcaacacg gagagcggtt ggggaccgaa tcgccgctta cccacgcgta
     9601 accgtaaggg gatttccaat cggcgctctt taggggtcat cggcgctctt tagcagtcgt
     9661 ctttaggggt cacccgttag ccggcaggtc aagtaggcac agaagttcct gcggagaaga
     9721 atttcttttt cttctcccgt tgattgaact cctcagtgtt tgatcgtccg ggttacgcgc
     9781 ccctgttttc aatcggtggg cgtcgatttg gtccgcaccg ttcacccatc aggatcaagg
     9841 catggagatt ttctcaaaga gagtttctcc cgcccctggc agaagttcac ctggtgccgg
     9901 gtcggaccaa ggcaacatct tgaattagca agctaagata gaatgaaagg catcgattga
     9961 gggcttagag gcagcttttt tgaatcaaaa acgcggcctc taagaacgaa catgccacga
    10021 gcaagccgtc actttctacc agggtacgtc tggcatatca cacaccgttg ccatcaaaga
    10081 gaatttctcc tcaagttcgc gcgtgatcgg cgacgctatt tatattggct gttcgaggca
    10141 aagaagcgtt acggcttgtc tgttctcaat tatgtagcta tgtcgaacca ttttcaccta
    10201 atggttaagg acacaggcgc aaacgttata gcggcgagca tgcagctcat cgccggccgc
    10261 accgctcaag agtacaacca gcgtaagggt aggcagcatt ctgggaggat cgtatcacgc
    10321 cacggccatc gaagcagacg tgcagctgca tcgctgtcta gcgtacattg atctcaacat
    10381 ggttcgcgct ggcgtagtgg acgatcccga gaaatggaag gaaagcggtt tttctgaaat
    10441 ccaaaagccg ccgaagcgtt atgccatcat cgacttacag aatttaatcg agctgagtgg
    10501 gtttgcagat gtgcgagatt atcaaactgc acatcgccag tgggttgagc aagggctgga
    10561 gaacggctta ttgatgcgcg atgatcgttg gacggaatcg attgcggtcg gaagcttggc
    10621 gtttattgac caggtgaaga acgagcttgg cttcaaagct gaccatcgcg atgtcatcga
    10681 atacgatgga agttgcgtgc tgcgggaggc agcggaaggc tacgcgtgca aatttgccgt
    10741 tgaaaatgag gctctaaagt ctcaaaacac gtttttttgg aatgaaatcg ttgatgaagc
    10801 aacgatatag cttggttcga cccgaaattg atcccattat ctgggaagat ggagccttgg
    10861 atgcatagaa gccgcgaccg accttgaggt ttcgaaactc tatttgacga cggttggttg
    10921 cttgacagtc tttccaggat tacgctatgc ggcgagtcgg accttgaatc ggccattgcc
    10981 gattccatga tcgcaagaat ccatgctgcc tttgccgctg gtttcgtggc gcgaaaaaac
    11041 cgcggaggaa atatgggaca actaccgagc ttaacgcgtg aacaactatc gcctcacgac
    11101 cggaccgtct gggatcacgt ctatagcggc cggactcttc cgcccggcgg cgggccttat
    11161 gccgtgctga tgcattcgcc gcagatggcc caacagtttt ccaacgtgga gaactactac
    11221 cgaaacagcg gtcagttgga tccggtcgat aaggaattga ttatcctcac gatcgcgcgg
    11281 gcaatgaatg tccgctatcc gtggggccgc catgagatac gcgcgcggca ggtcggcatg
    11341 cgttccgagg cgctcgagac actccgcgcc aacgctccgc tcgatgcttt gaacgaccac
    11401 gagcggctca tcgtcgaaat gacgcgcgcg ctcttgcacg agcgcaaatt acccgaggag
    11461 atgtttgccc gcgcaagagc tgaattggga gaccagcggc tggtcgaagc ggtcggcctg
    11521 gttgggcatt ataatttcat cagcatggtc gcgcgcacat tcgatttaga tgtgccgccg
    11581 ggcacaatga ccttctagaa tttaagccgt gcagatcgga ccaaggcaaa ttctcgattg
    11641 agcaacgtaa gatagcatca aaagcattcg tttaagcgct ggctacgcct gttcaaaatt
    11701 caggaaatta actttcggga cttctaacta tgggatctct cgattatcgc cgcgccggta
    11761 tttgtcttgc gttttcgaag caaagaaacg gttcggtctc tctgtaccga actacatggt
    11821 gacatccaac cacgtccatc tcttggtgaa agacacaggc ggagatgtca tagcccagag
    11881 catgcagtta atcgccggcc gcagtgggca agaatacaat cggcggaaag gacaccacgg
    11941 cgccgcattc tgggaagatc gctatcatgc tacggccgtc gccgttgacg agcacctgca
    12001 ccgttgtctt gttttcatcg acttgaacat ggtgcgcgcc ggcgtggtca agcacccgct
    12061 tagctgggtg tacagcgggg tctgtgaggt cgaacaatgg ctcacgcgtt agcggctgat
    12121 cgacttacca gccttgcttg cactgtgcgg cttttcgaat caggcagagt ttcaacaagt
    12181 cggcgccggt gggtggacca agctcttcaa ggcgcgttgc cgatgcgacg cgcggtggac
    12241 ggaagcactt gcggttggca gtcagcgttc gtcgagaagg ttaaagaaaa gttaggaccg
    12301 aaagcgttgc atcgcgaact cgacagggtg gatggaacat atgcgttacg agaatctggc
    12361 gaagcttaca gcagccattt taccgtcgaa aatgagcctc tta
//
LOCUS       DASUVS010000435        14271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1287330, whole genome shotgun sequence.
ACCESSION   DASUVS010000435 DASUVS010000000
VERSION     DASUVS010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14271
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287330"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VF182_22880"
     CDS             <1..320
                     /locus_tag="VF182_22880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439981.1"
                     /translation="NFVKSYIESVHRLKTDRAFGLKIAAKYLRLEDKELLQRTYESSV
                     DEKKLPAKQYPSVAGLKTIIDQIGATNPKAKTAKPEDFIDARFVEEFDRSGYIDSLYG
                     RQK"
     gene            complement(368..1399)
                     /locus_tag="VF182_22885"
     CDS             complement(368..1399)
                     /locus_tag="VF182_22885"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6439982.1"
                     /translation="MKKLISCLSLLAVIAGTGYAASDEFYKGKTIRLVIGNSTGGAMD
                     DWGRFVAQHLGRHIPGNPEILVQNMPGAGAIVAANYIYNIAKPDGLTLGLVNPANYTD
                     QLLGAKEVKFDWPKFSWIGSPERIDQVLFIRTDVPYRTLDEMRNAKDPPRCAALARAG
                     LGYLLPRLFEEALGIKLNMVLGYGGGGEMNLAIEKGEVHCRAGTVSAYVGREPTRTWI
                     KNGVVRAITQSGTKRYPKLPDVPTLYELMDAHKTPDATKRVARVLLSSGDHGRPFIAP
                     PAMPADRVKVLRDAFNKAMTDPALMADAQKRKWDLDPLTGEELEATAKEIMVQPPEVI
                     ERMKKLLEN"
     gene            complement(1578..2384)
                     /locus_tag="VF182_22890"
     CDS             complement(1578..2384)
                     /locus_tag="VF182_22890"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEX6439983.1"
                     /translation="MVEAVTVDIPYTPDTGEKLVNETFGPNNIHRRKTGADELKSMTV
                     RNGRPLAGRLSLDEQGFVFVEHKTKVADFFDAEQLQSVYYPEVEELIKSASGAARVVV
                     FDHTLRSGDQTEREAKLIREPVLSAHNDYTEWSGPNRLREVLPDEAESLLERRFAIIQ
                     VWRAINQPIQSNPLAVADAKSVALDDFMIAERRYPHRVGQTYRLKYNPSHRWFYFPEM
                     RRDEALVFKVYDSETDGRARFTPHTSFKDPASPVNARPRQSIEMRAFSFF"
     gene            complement(2556..3749)
                     /locus_tag="VF182_22895"
     CDS             complement(2556..3749)
                     /locus_tag="VF182_22895"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6439984.1"
                     /translation="MTTEKSASIWTRAFALLCLTQFLGYAQHFMLAPVLPLYVTDLGG
                     SAFVVGVVLACFAATSVIIRPLVGHWADRWSETGVLISGLLFQGASVFLCLIPLIEAV
                     MLANALRGLGWAGLNTGGYSLLALTAPESRRGEASGLYSGVQGAASILFPAVALWLLA
                     ASFGGFNLVFVTTALLSLLAAGVGAVMLRYAPRVIREPQLEQGRPWWREVFHFFEPEI
                     FLPSILLLWLNLALPSMTNFIVLYARELAVENFGIYFIICGITNLLARPGLGRVSDKI
                     GRGRSVAAGFMLQVLALSLITLASGLPGILIAGVLYMLGNAIGSATILALAVERADPR
                     RRGKAMATFSMAYPLSYGVGSLMIGSIVEVAGYVGMFLILAAVEAGGLIFVHLNQRTL
                     RSGTN"
     gene            4040..4732
                     /locus_tag="VF182_22900"
     CDS             4040..4732
                     /locus_tag="VF182_22900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX6439985.1"
                     /translation="MAEKRSGAKEIQFVVSETTGVVSGLLLKPEGAVSLLVFAHGAGA
                     GMRHRFIEDLSQKLARLGVATMRYQFPYMEKRVKRPDSEGVLTATVQAALGAADKLSD
                     GMPIFAGGKSMGGRMTSLALAKAPHDKVQGLIFFGFPLHAPGAPSAERGKHLTDIHVP
                     MFFVQGSRDALADLKLLKPLCAELGERAELFVVDGGDHSLHMSKRSGRSDAVVLDEVA
                     AKTLSWMAARNA"
     gene            4824..5312
                     /locus_tag="VF182_22905"
     CDS             4824..5312
                     /locus_tag="VF182_22905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6439986.1"
                     /translation="MFANEEEALRVLKDQDPLLAANAEAYLWRQWCQCGELEADRVFR
                     AAVDAMQKGELELAETLFGKVIENAPHFAEAWNKRATVRYARQNFAASISDCQETLIR
                     NPNHFGASAGQGLCHMALDEYREAAICFRRALEIHPHLHAVRQNLVLAQSEASVDGGY
                     LH"
     gene            5645..6673
                     /locus_tag="VF182_22910"
     CDS             5645..6673
                     /locus_tag="VF182_22910"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6439987.1"
                     /translation="MKKIFLALILVISSMAVAHAQAPLFQGKTITTIVGYLPGDGYDI
                     WARLLAAHMPKHIPGSPGMIVQNMPGAGSMIAANYIYNVAKPDGTTIGSIGPSLYFDQ
                     LVGKKEVSFDWAKFGWIGSTENTPWLLYMKADTPFKSIEDIRKASQAPKCSATSTGTS
                     GHFIPKLLEEAFGAKFDMVMGYKGGAEQDLALERGEVVCRSLSIPTFFAREPFITWRK
                     KNIVRILMQTGRKRDSRMPDVPTIYELMEEYKTPPQTRSLVTAILASGDLGRPFIAPP
                     NLPADRLKILRDGFQKTMTDPAFLADVKTRKLEADPDFGEQLETIAKEAVNQPREVVE
                     RMKKLLGD"
     gene            6875..7426
                     /locus_tag="VF182_22915"
     CDS             6875..7426
                     /locus_tag="VF182_22915"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX6439988.1"
                     /translation="MLQPLCPNCSRDYTKRVSRVGFKERLLSIIYVYPFRCQLCGYRF
                     RVMQRGVRYTRVEEDRREYNRMPTNFPVSFAYDGIEATGHVVEISLNGCSLEADTVIA
                     EGSIVRMALQMSEELAPISVEAALVRDINRNRIGIEFLRFQYHDRERLQIFIRRLLNG
                     IPEPSPRPSILLSALKRTRHIGA"
     gene            7763..8875
                     /locus_tag="VF182_22920"
     CDS             7763..8875
                     /locus_tag="VF182_22920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439989.1"
                     /translation="MFICLSPGGQSITQGEAPKEKLLVGTVDGIFAFQKRNGSWKGQG
                     TMVPGKHFSSIIFEPVTQTLFGGTYSGEIYASADQGQSWERRDNGIDDKEIYSIATQV
                     VDGQPRLYAGTQPAHLFYSDDLGKSWTELPGLRQVPGVEKWTFPGPPHLAHAKSITFH
                     PKDPNIIHVAIEVGGFLKSMDGGKTWSTVDNINPDAHRVLIPTNDPSKLYGTAPTTNC
                     GPETAAGFCVSEDGGENWKSMTPRDFRIGYVDPFFVHPSDPNLLFVAGAKTGPGTWRK
                     LHTADARIARSRDGGKTWDILTGGLPEHIRANIEGMAMDAWNGGYALFAGTTDGDIFY
                     SDDEGEHWQTIVSGIGPVSKSHHHINLSLDEQAAHH"
     gene            9349..9669
                     /locus_tag="VF182_22925"
     CDS             9349..9669
                     /locus_tag="VF182_22925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX6439990.1"
                     /translation="MSKFLMQVSFTREGTRGLTEEGGTKRRQKVEQFFGSVGGKLEAL
                     YFAFGETDVFAIVDFPDNVSAAAISLAANSTGAVHAKATVLITPEEMDQAAEKSEGLR
                     PPVS"
     gene            9843..10661
                     /locus_tag="VF182_22930"
     CDS             9843..10661
                     /locus_tag="VF182_22930"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HEX6439991.1"
                     /translation="MPIITIYQGASGEGQELAEAVAQALGYRCVGREVLLDASGRYRI
                     PEAKLNAVVEKGPHWWERLLQDLRPYRTALQASLCELAQNGKVVYHGHLGHELLSGIG
                     HVLKVLLTAPLEFRIEQVRARQNLRDAAARRYIEEVDKARSRRLMAMFGADWRDPNRY
                     DLILNVGRMSREGAKHVIVEAAKLEEYQPTPASTQAFKDLDLGSRVQATLFASPELRR
                     SSLEVRAQGGHVHVTGRVDYGLEDKVLMLVKNVPGVIRVTSEVYSPPPEGYFGP"
     gene            10826..12076
                     /locus_tag="VF182_22935"
     CDS             10826..12076
                     /locus_tag="VF182_22935"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX6439992.1"
                     /translation="MTGSTEHVETVIIGGGHAGLSMSYFLSQLGREHVILERGRVGER
                     WRSERWDSFCFQFPNWTIELPGYKYQCADPEAFAPGREVVRFIEDYATYIKAPTRCGV
                     EVASLARTEDSSRYLLTTNNGAIQATNVVIATGPYQLRVIPAISAHVPNNILQVHSSE
                     YRNPNQLPPGAVLVVGSGASGCQIADDLNQSGREVYLSVGRHSRMPRRYRGQDFAYWG
                     AALRRPEQIVDTVPVNLRKGSEVLLTGANGGYDVDLRAMAACGIVLLGHLHDIKDGNL
                     VLADDLAQNLAKGDDALENFTTAVDDYIAKNGLEVPQEQRSKLDPNIAKEIPAPTLNL
                     NLKDAGVSAIAWASGFRCNFDWVKLPIFDEAGEPVHRRGVTQLPGIYFLGLRWLYKRK
                     SAFLLRASGAEDATYLTEQIMARN"
     gene            12222..12380
                     /locus_tag="VF182_22940"
     CDS             12222..12380
                     /locus_tag="VF182_22940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439993.1"
                     /translation="MQTFSIVEAHYDPESYGASVIVITRDSALKHGMPAEAVAAWEQD
                     LRSHTAEV"
     gene            12820..14067
                     /locus_tag="VF182_22945"
     CDS             12820..14067
                     /locus_tag="VF182_22945"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX6439994.1"
                     /translation="MNEKIDTVIIGAGHAGLTMSYFLSERDLEHVIVERGRVGERWIS
                     ERWDSFHFQFPNWTIELPGYKYQTEDPDGFVPGREIVRFIQQYADFIKAPVRSGVAVT
                     ALENSSNSMPYLLRTNAGAIEATNVVIATGQREKGVIPPFSSDVPHDIHQLHSSAYRN
                     AGELPPGAVLVVGSGASGFQIAEDLHQNGRQVSVCMGRHRRLPRRYRGRDYAWWAMKL
                     GVFDRRRAEMSAISQPFALLTGVNGGYEADWRVLASKGIVLLGRLTAVKGTKLALAPD
                     LEENLAKGDEWLANFKKLVDDYVAKNKLDFPTDSSPRLEGGASAPILELDLSSAGITS
                     IIWATGFCNDYEWLKLPLLDDRREPVHERGVTQFPGIYFLGLRWLYKQKSGFLSFGGP
                     AEDAAYLAEQIAARHNGRPSRSS"
BASE COUNT         3246 a         3826 c         4084 g         3115 t
ORIGIN      
        1 gcaacttcgt caaatcgtat atcgagtcgg tgcaccggct caaaaccgat cgcgcatttg
       61 ggttgaagat cgccgccaaa tatctgcggc tcgaggacaa agagctgctg caaagaacct
      121 acgagagttc ggtcgacgag aagaagctgc ccgccaagca gtacccatcg gtggcggggc
      181 taaagacgat catagatcag atcggcgcca ccaatcctaa agcgaagacc gctaagccgg
      241 aggacttcat cgatgcccgt tttgtcgaag aattcgacag gagcggatat atcgacagtc
      301 tctacggccg ccagaagtag gatcgagctc tgctagtcaa ccatagaggc gcgctctatg
      361 gccggcgtca attctccaac aacttcttca tccgttcgat gacttccggc ggctgcacca
      421 tgatttcttt ggcggttgct tccaactctt cgcctgtcaa aggatctaga tcccattttc
      481 ttttctgcgc gtcggccatc agcgccggat cagtcatggc tttgttaaaa gcgtcgcgca
      541 aaaccttgac ccgatctgcc ggcatcgccg ggggcgcgat aaagggccgg ccgtgatcgc
      601 cggatgaaag caatacccgt gcgacccgct ttgtggcgtc gggcgtttta tgcgcgtcca
      661 tcaattcgta gagcgtcgga acatccggca gttttggata acgctttgtg ccgctttggg
      721 tgatagcgcg aacaactccg ttcttaatcc atgtccgggt tggttcccgg ccgacgtaag
      781 ccgaaaccgt tccggcacgg cagtgcactt cgcctttttc aatagccagg ttcatctcgc
      841 cgccgccgcc atagccgagc accatgttga gcttgatgcc gagtgcctcc tcgaacaggc
      901 ggggcagaag gtaacccaat ccggcgcgcg ccaatgccgc gcatctcggc ggatctttag
      961 cattgcgcat ctcgtccaag gttctatagg gcacgtcggt gcggataaaa agcacctggt
     1021 ctatgcgttc cggcgagccg atccaggaaa atttgggcca gtcaaacttc acttccttgg
     1081 cgccgagaag ctgatccgtg tagttggcgg gattgaccag cccgagcgtc agcccgtcgg
     1141 gcttggcaat gttgtaaata taatttgccg cgacgatcgc tcccgcgccc ggcatgtttt
     1201 gcacgaggat ttcagggttt ccgggaatgt gccggcccaa atgctgagcc acaaaccggc
     1261 cccagtcgtc catggcgccg ccggtcgagt tgccaatgac cagccggatg gttttgcctt
     1321 tgtaaaattc atccgaggcc gcataaccgg tgccggcgat cacggccaag agagacaagc
     1381 aagagatgag ttttttcata aggacccttc atgaaatcag gatgaaagct gaaagcggaa
     1441 agagaaacac gtggtgacat gatcgtggaa cctgttgtca ttttctatgt agactttcct
     1501 ctttccctga ttcggcgatc acgagcacgt tagcacgaag acgagagcag acagctttca
     1561 tcgctagccg gccagggcta gaaaaacgaa aacgcgcgca tctcaatgct ttgacgcggc
     1621 cgcgcattga ccggcgaagc cggatctttg aacgatgtgt gcggcgtaaa ccgcgcccgt
     1681 ccgtcggttt ccgaatcgta gaccttgaat accagcgctt cgtctcgcct catttcagga
     1741 aagtagaacc accgatgact aggattgtac tttagccggt aagtctgacc tactcgatgc
     1801 ggataacggc gttcggcgat cataaaatcg tcgagcgcga cactcttggc gtcggcaacg
     1861 gccaggggat ttgactgaat cggctgattg atggcgcgcc acacctgaat gatcgcgaag
     1921 cgacgctcaa gtaaggactc cgcctcatcg gggagaacct cgcgcagccg gtttgggccg
     1981 gaccattcgg tgtagtcgtt atgcgccgag agcaccggtt cgcggatgag ctttgcctcg
     2041 cgttcggtct gatcgccgga gcgcagagta tgatcgaaga ccaccacgcg cgcagcgccc
     2101 gaggcgcttt tgatcagctc ttcgacctcc gggtagtaaa ccgactgcaa ctgctcagcg
     2161 tcgaagaaat ccgcaacctt ggtcttgtgt tcgacgaaca cgaacccctg ctcatcgagc
     2221 gacagccgcc cagcgagcgg cctaccgttg cgcaccgtca tcgatttcaa ctcgtctgct
     2281 ccggttttgc gccggtggat gttgttcgga ccgaaggttt cgttgaccag cttctcgccg
     2341 gtatcgggtg tatagggaat atcaacagtc acggcttcaa ccataaaatt ccctccttga
     2401 ttgcgtcacg cttatcacac gggagctgcc gatgaaaagg tctcaatgaa agggcaggaa
     2461 gctgaagcag gtcaaaccga ttgagtgccc aaaccttttg agccggatta gccgatagca
     2521 atcgcatctg gacccatcaa tcggtgactg ccgattcaat tcgtgccgga tctcagggta
     2581 cgctggttca gatgaacgaa aatcaaaccg ccggcctcaa ccgcagcgag aatcaagaac
     2641 atcccaacat agccggcgac ttcgacgatg ctgccgatca tcagcgaacc gactccgtaa
     2701 ctcagcggat acgccatcga aaacgtcgcc atcgctttgc cgcggcggcg cgggtctgcg
     2761 cgttccacgg caagcgctaa aattgttgcg ctgccaatcg cgttgccgag catatataac
     2821 acgccggcaa tcagtatccc gggaaggccc gaggccagag tgatcaatga taaggcgagc
     2881 acttgcagca taaatcctgc tgcaacggag cggccgcggc cgattttatc cgacacccgg
     2941 cccaagcctg ggcgcgcgag caaattggtt atgccacaga tgatgaagta gatgccaaaa
     3001 ttttctacgg ccagttctcg ggcgtatagg acgatgaaat tcgtcatgct cggcaaggcg
     3061 aggtttagcc acaggagcaa aatcgatggc aggaagattt ccggttcgaa aaagtgaaaa
     3121 acctcgcgcc accacggccg tccttgttcg agttgcggct cccgaattac ccgcggcgca
     3181 tatcggagca tcaccgctcc aacgccagcg gccagcagcg agagcagcgc ggtcgtgaca
     3241 aagaccaggt tgaatccgcc gaaggaggct gccagcagcc agagcgcgac ggctgggaat
     3301 aaaatcgatg cggcgccctg gacgccgctg taaaggcccg atgcctcgcc ccgcctggac
     3361 tcgggagccg tgagcgccag caatgaatag ccgccggtgt tgagaccggc ccagcccaat
     3421 cctcgcagcg cattggctag catcaccgcc tcaatcagag ggatcaaaca gagaaataca
     3481 ctggcgcctt gaaacagcaa tccgctgatg agcacgccgg tctcgctcca gcgatccgcc
     3541 caatggccca cgagcgggcg gataataaca ctggtagcgg cgaaacaagc caggacgact
     3601 ccgacgacga acgccgatcc gccgagatcg gtcacgtaaa gcggcaagac cggcgccaac
     3661 atgaaatgct gcgcatatcc caagaattga gtcagacaga gcagcgcaaa cgcgcgcgtc
     3721 catatggagg cggatttttc ggtagtcacg gagttgaaaa ctctaactcg atttcgctcg
     3781 cgtaatgaca aactcaggcg cggattataa gaatctgtaa gctagcacga tcaaaacatt
     3841 tgatcgaggc gagcaggatt ggctcgatat cgcatgcaag ttgagcaatg atgctggcat
     3901 tttcttaaag gattttaatg gctgctccga tccgcgcgca gttatcgttg ccgtggtgct
     3961 gctttcgtta tagtttccga atggaacgat taaagaacta gccggcgtgc tcgaaattaa
     4021 aacttcttgg aggataagga tggctgaaaa gcgatccggg gctaaagaaa ttcaatttgt
     4081 cgtaagcgaa accaccgggg ttgtgtcggg actgctgttg aagcctgagg gcgctgtctc
     4141 tttgctggtg tttgcgcatg gagctggagc cggtatgcgc catcgcttta ttgaggatct
     4201 ctcgcaaaaa ttggctcgac tcggcgtcgc taccatgcgt tatcagtttc cctatatgga
     4261 aaaacgagtc aaacggcccg attccgaagg agttctcacc gccacggttc aagccgcgct
     4321 gggcgcggct gacaagcttt ccgacggcat gccgatcttc gccggcggca aatcgatggg
     4381 tggccgaatg acttctctcg cgcttgcgaa agcgccgcac gataaggtcc agggtctgat
     4441 tttttttggg tttccgctgc atgcgccggg agcgccatcg gcggaacgag gcaaacattt
     4501 aaccgatatc cacgtgccga tgttttttgt ccagggctcg cgcgatgctt tggctgatct
     4561 taagctgctg aagccactct gcgccgagtt gggggaacgt gctgagcttt tcgtcgttga
     4621 tggcggcgac cattcgcttc acatgtcgaa acgttccggc cggagcgatg ctgtagtcct
     4681 cgacgaagtc gcagcgaaga ctttgtcgtg gatggcggcc aggaatgctt gatgatttag
     4741 atgcatcatg ttagtcgcct ttgattgctg cggcaaggtc atattggcga gagatctcta
     4801 tgcttagctg agaggtggtt tcaatgtttg caaacgaaga agaagcgttg cgcgtcctaa
     4861 aagaccagga cccgctcttg gccgccaatg ccgaggcgta tctctggcgg cagtggtgcc
     4921 agtgtggcga gctggaggca gatcgagttt ttcgtgccgc tgtcgacgcg atgcaaaaag
     4981 gcgagctcga gttagcagag acgttgttcg gcaaggtgat cgagaacgcg ccgcacttcg
     5041 cagaggcatg gaacaagcgc gcaacggtgc gttatgcgcg gcagaatttc gccgcatcga
     5101 tcagtgattg tcaggaaact ttaatacgca atcccaacca cttcggcgcc tcagccggcc
     5161 aggggctgtg ccatatggcg ctggatgaat accgtgaggc ggctatctgc tttcgccgcg
     5221 ctctggaaat tcatccgcat ctgcacgccg tgcgacagaa ccttgtcctg gcgcaatccg
     5281 aagcatcggt tgatggagga tatcttcact gaagggaacg atcttgacag gttcagctct
     5341 gctgttgcga tgcttgcgcg agacagtgcc gaactgattc agtcgcttcc gatagtctct
     5401 atccgatcaa atagttcgcc tgcagtagcc aaaattcttc gcataacggc cggcgtcaga
     5461 cttcggatcg aatcaggtgc ttgtaggtcc ttttccgcct gagaccaacc acgttgaacg
     5521 ccaacaatta gtccgctata gctggattcc cgggcgatga tcgcaaactc tcgggggggt
     5581 agctctgtga gctgagcact cgcttgatct aagcattgca tgcgtgacaa atagaaaggc
     5641 gatgatgaag aaaattttct tagcgttgat attggtaatc tcgagtatgg cggtggcaca
     5701 cgcacaggct cccctctttc agggaaaaac gattacaaca attgtcggtt acctgcccgg
     5761 tgacggctac gacatctggg cgcgcctgct ggcggcgcat atgccgaagc acattcccgg
     5821 ctctcccggc atgatcgtgc agaacatgcc cggcgccggc tcgatgatcg ccgccaacta
     5881 catctacaac gtcgccaagc ccgatgggac gacgatcggt tccatcggcc cgtcactgta
     5941 tttcgaccag ctcgttggca agaaggaagt gagcttcgat tgggcaaagt ttggttggat
     6001 cggctccacc gaaaacacgc cgtggctgct ctatatgaaa gcagacacgc cgtttaagag
     6061 catcgaagac attcgcaagg ccagccaggc tcccaagtgt tcagctacca gtaccggcac
     6121 gagcggtcat ttcattccca agctgctcga ggaggcgttc ggcgccaaat tcgatatggt
     6181 gatgggctac aaaggcggcg ccgagcagga tctggcgttg gagcgaggcg aggtcgtttg
     6241 ccgctcgctg agcattccaa ctttttttgc ccgtgaacca tttatcacct ggcgcaagaa
     6301 gaatatcgtc cgaatactaa tgcaaaccgg ccgcaaacgt gattcgcgca tgcccgacgt
     6361 gccgactatt tatgaactca tggaagagta caaaacaccg ccgcaaaccc gatctctggt
     6421 gaccgcgatc ttggcatccg gagatttggg acgaccgttc atcgcgccgc cgaacctgcc
     6481 ggccgatcga ttgaagattc tccgcgacgg tttccagaag accatgaccg atccggcttt
     6541 tctggccgac gtgaaaaccc gaaaattgga ggccgatccg gatttcggcg aacaactgga
     6601 gaccatcgcc aaagaagcgg ttaatcaacc tcgtgaggtc gtcgaacgca tgaagaagct
     6661 acttggcgat tgagcaggcc gccgcgtgtt gttccccctg cggattgacc catcgttcaa
     6721 tccgttctta ggcgattgcg aacgcaattc cgccgaatcc ttgtgatcgt ttgccgtcgg
     6781 gccgcgttgt atctggcgga acatgacttg cattggcaag cccttaccct tgtgaaagcc
     6841 atcgctggca aggtcaagga cgggaaaaga gtcaatgctt caaccgctat gtccgaattg
     6901 ttcgcgcgat tacaccaaac gggtttcgcg ggtggggttc aaagaacgtc tgctgagcat
     6961 catttatgtc tatcctttca ggtgccagct gtgcggttac cgcttccgtg tcatgcagcg
     7021 gggagtgcgc tacacgcgag tcgaggaaga tcgtcgtgag tataaccgga tgcccacgaa
     7081 ttttccggtt tcgtttgcct atgatggcat agaggcgacg gggcacgtgg tggaaatttc
     7141 tctaaacggt tgctcgcttg aggctgacac cgtgatcgcc gaaggtagta tcgttcgtat
     7201 ggctttgcag atgtcggaag agctggcgcc aatcagtgtc gaggcggcac tggtgcgcga
     7261 tatcaacaga aatcgtatcg gcatcgagtt cttacgtttt cagtatcatg accgtgaacg
     7321 tctgcagatt tttattcgtc gtctgctcaa cggaattcct gaaccttctc caaggccgtc
     7381 aattctacta agtgccctga agcgcacccg tcacatcggt gcgtgatcga ttgttttcgc
     7441 cgccctgatt cgtatgcttg ccggtctgct tctggctagg cgcgggtaac tttctgcatc
     7501 ccaaccagat agtgacgacg atagctatgg ataagcatgc accgcgaggc agcaagtctt
     7561 atgcttgaat atcaactctt tactgagagt cggcttggga gattgcgatc agatgcccac
     7621 tgacttccat tcccgcgcgc cataactagc atgccgtcga ttgtttgtta tccgccttga
     7681 cgaagccgcg cgtggttcag tagagtggct tcagatcgaa caaaataaac ctactctccc
     7741 aactctataa ggaggccacg ctatgtttat ctgtctatca cccggagggc agtcgatcac
     7801 tcagggcgag gcgccaaagg aaaaattact cgtcggcaca gtggatggca ttttcgcttt
     7861 tcaaaagcgc aacggctcgt ggaaaggtca ggggaccatg gttccgggaa agcatttcag
     7921 ctcgattatc ttcgagccgg taacccaaac gctgttcggc ggtacttata gcggtgaaat
     7981 ctatgccagc gcggatcagg gccagagctg ggagcgccgt gacaacggaa tagacgataa
     8041 ggaaatttac tctattgcga cccaagtggt tgacggccag ccgcggctat acgcaggtac
     8101 tcagccggcg catctgtttt acagcgacga tctcggcaaa agttggacgg agctgccggg
     8161 gctgcgccaa gtgccaggtg tcgagaagtg gactttcccc ggcccaccgc acctggcgca
     8221 tgccaagagc atcacctttc atcccaagga tccaaacatc atccacgtgg ccatcgaagt
     8281 gggcggattt ctcaaaagca tggacggcgg taaaacctgg agcacggtcg acaacatcaa
     8341 ccccgacgcg catcgggtgc tgattccgac caacgatccg agcaagctct acggcaccgc
     8401 gccaacgacc aattgcggtc ctgaaaccgc tgcggggttt tgcgtcagcg aagatggcgg
     8461 cgagaactgg aaaagcatga caccgagaga ttttcgcatc ggttacgtcg acccgttctt
     8521 cgtccaccct tcggatccca atctgttgtt cgttgccggc gccaagaccg gcccaggcac
     8581 ctggcgcaaa ctgcacaccg ccgacgcgcg catcgcgcgc agccgcgacg gcggcaagac
     8641 ctgggatatt ctcaccggcg gcttaccgga acacattcgc gccaacatcg aaggaatggc
     8701 gatggatgcc tggaacgggg gctatgcttt atttgccggc accaccgacg gcgatatttt
     8761 ttacagcgat gacgaaggcg agcattggca gacaatcgtc agcggcattg gcccggtttc
     8821 caaatcgcac caccacatca atttatcgct agacgaacag gcggcgcatc actagtagtt
     8881 aaataaagcg ccttgcattg ggcgggagcg gcatgctgct cccgccgatc tttcttctcg
     8941 ttaactccgg ttttgatgca gtcgcacggg cgcacgccgt gccgtacata tgctaggcca
     9001 cgaactcgcg tttgagcctt gcaatacggc gagaaattgc ttgacagact ttcctgccgt
     9061 ggtagtttcc cgatcgtaaa agcctccccc atcgttgata aggcttgatc tgaggccgca
     9121 gaggaacgga cgtcgctcct cggaggggga atgaacaagt gtttatggac cagctgctcg
     9181 ggtgctaata gcgccagggt gtgctggttt tttattggtt gccttaagga aagttggtgc
     9241 cgcaacttgc gccaagtaac actcaccgag cacagcccat cggcgaacgt ggaccttctc
     9301 gacggacgag aggagcagga taacagagtt ccgtaacaag gaggttttat gtccaagttt
     9361 ctcatgcagg tatcatttac cagagaaggc accagaggac ttaccgaaga aggcggtacc
     9421 aagcgccgcc aaaaagtcga gcaattcttt ggtagcgttg gaggcaaatt ggaagcgtta
     9481 tatttcgcat ttggcgagac agatgttttt gcgatcgtcg atttcccgga caacgtgagc
     9541 gccgcggcga tttctctggc tgcaaattct accggagccg ttcatgcgaa agccacggta
     9601 ctcatcacgc ccgaagagat ggaccaggcg gcagagaaga gcgaaggcct tcgcccgcct
     9661 gtctcctgag cgattacgcc agggcaaaag tctgagaatc atactgggcg agttgaaaag
     9721 cgattcacgc atttgtgcga gagttttgcg ctggcgtttg ttaaaggcag tgtgaggttc
     9781 aggatcgttt tggatttctg gtggaggttc gatcatgttt aaaccttaga gaggtcgctg
     9841 ttatgccgat catcacaatc tatcagggtg cttccggtga aggtcaggaa ttggccgaag
     9901 ccgtggccca agcccttggt tatcgttgtg tcgggcgtga agtgctcctg gacgcgagtg
     9961 ggcgctatcg tataccggaa gctaaactca acgcggtggt cgaaaaagga ccgcactggt
    10021 gggagcgtct attgcaagat ctgcggccct atcggactgc gcttcaagca tcgctatgcg
    10081 agctggctca aaacggcaag gttgtttacc acggccactt gggtcatgag cttttgtcag
    10141 gcatcggcca cgtgctgaag gtgcttctta ccgcgccgct ggagtttcgt attgagcagg
    10201 tacgagctcg gcaaaatcta agggatgcgg cggcgcgccg atatatagaa gaggtcgaca
    10261 aagcgcgcag ccgtcggctc atggcgatgt tcggcgcgga ttggcgtgat cctaatcggt
    10321 acgatctgat tctcaatgtg ggcaggatga gccgagaggg ggcaaagcat gtgattgttg
    10381 aggcggcgaa actcgaggag taccaaccga cgcccgcatc cactcaggct ttcaaggatc
    10441 tcgatctcgg ttcacgggtg caggctacgt tgtttgcatc gcccgagcta cggaggtcgt
    10501 cgctcgaagt tcgcgcgcaa ggcggccatg ttcacgtcac cggtagagtc gattacggat
    10561 tggaagacaa agtcctaatg ctagtcaaaa atgtaccggg cgtgatcagg gtgacatccg
    10621 aggtttattc tccaccgccg gaagggtatt tcggaccctg agtgcagccg aactgctccg
    10681 gttgttcttg ccgtggcgag attgagcgat gactttctcc gctcatccag cagaaggaca
    10741 cacacggact gagcatcttt gcgttcagcg agctcagcga acaatggcat ccatgagctc
    10801 gagggaaaga gagaggaggt tatttatgac tggaagcacg gaacacgttg agacggtcat
    10861 catcggcggc ggtcacgccg gcctgagtat gagctatttt ctcagtcaac ttgggcgaga
    10921 gcacgtgatc cttgaacggg gccgagtagg tgaacgatgg cgcagcgaac gctgggattc
    10981 cttctgcttc caattcccaa actggaccat tgagttgcca gggtacaaat atcaatgcgc
    11041 cgatccggag gcttttgcgc cgggcaggga ggtcgtccgt ttcatagaag actacgcgac
    11101 ctatatcaag gctccgacgc gttgcggagt ggaagtcgct tcactcgcgc gaacagagga
    11161 ttccagccgc tacctgctca cgacaaataa cggagcgatt caagctacca atgtcgtgat
    11221 cgccacgggt ccgtatcaac ttcgtgtgat cccggccatc agcgcccatg ttccgaataa
    11281 tatccttcag gtacattcca gcgaataccg aaatccgaat cagttgccgc caggtgcggt
    11341 gctggttgtc ggcagcggag cttcaggttg tcagatcgcc gacgacctaa atcaaagcgg
    11401 ccgcgaggtc tatctctcgg tgggccgaca tagtcgtatg ccgagaagat accgtggtca
    11461 agactttgcg tattggggag cggcacttag aaggcccgaa cagatcgttg atacggtgcc
    11521 ggtaaacctc cggaagggat cagaggtttt gcttacgggt gccaacggtg gctacgatgt
    11581 cgatttacgt gctatggccg catgcggaat agttttgtta ggtcatttgc acgatatcaa
    11641 agatgggaat ttagtattag ctgatgatct tgcgcagaac ctcgccaaag gagacgacgc
    11701 actcgaaaat tttacgacgg ccgtagacga ttatattgcg aagaatgggt tggaagtgcc
    11761 acaagagcaa aggtcgaagt tagatccgaa catcgcgaag gaaataccag ctccgaccct
    11821 aaacctgaat ctcaaagatg cgggtgtcag tgcgattgcc tgggcaagcg ggttccgctg
    11881 caacttcgac tgggtgaagc tgcctatttt cgacgaggct ggcgagccgg tacatcgccg
    11941 cggtgtgact cagttgcccg gcatctattt tcttggatta cgttggctct ataagcgtaa
    12001 gtcagctttt ctgcttcgag cctcaggcgc cgaagacgcc acttacttga cggagcagat
    12061 catggctcgc aattgatgct agcatcgata atttagtgct gacgcgcgaa agcattccaa
    12121 ggagcgtcgc gccatgaacg caggcacacg ccttatcacg ctgaggacat cagctggccc
    12181 gccacaagta tttgcgctct tcgggcttga cgcttgccga tgtgcaaaca ttctcaatcg
    12241 tcgaagcaca ttatgatcct gagtcgtacg gcgccagcgt gatcgtcatt actcgcgact
    12301 cggcgctcaa gcatggtatg ccggccgaag ccgtcgccgc ctgggaacaa gatctacgct
    12361 cgcatacggc cgaagtttaa gccggcgcac ggttaccggt gcgtgagata gcatacgttt
    12421 caaccgcgta ggttattact gatgtgtcta tgattcggca atgtctcggt cgccttttac
    12481 atagaaccag agctggggta acctttttat taccgtcgca ttctcgctcg tagtctaaag
    12541 gttacccttc tttcagtgat cgtcctcatc ttgtgactgc cacagctgga tttcctcgac
    12601 agatcaagtg gttcggttat atgtgggccg gctagacttt atggcgcatg cgttgctcta
    12661 cgctttttgt acggttgaca ctgaagttcg tggtcgaata ggctcgccca aggccacgag
    12721 gggaaaaagt tggatatctc accgcagagg cacggagagc gcagagaaag ggttctggac
    12781 taaaatcatc tgaccttcgc ggcgtctagg gagatcgctg tgaacgaaaa gatcgatacg
    12841 gtcatcatcg gcgctggcca cgccggcctg acgatgagtt attttctcag tgaacgcgat
    12901 ctcgaacatg tgattgtcga gcggggacgg gtgggtgagc gttggatcag cgaacggtgg
    12961 gactcgttcc attttcaatt tcccaactgg accatcgagc tgcccggcta caaatatcaa
    13021 actgaagatc ccgacggctt tgtcccgggc cgggaaatcg ttcgcttcat tcaacaatac
    13081 gccgatttca taaaagcccc agtgaggagc ggcgtcgccg ttaccgcgtt ggagaactcg
    13141 tcaaattcga tgccctactt gctgcgcacg aacgcgggcg ccattgaagc caccaatgtg
    13201 gtgattgcca ccggacagcg cgagaagggt gttattccac cgttcagttc cgatgtgccg
    13261 cacgacatcc accagctgca ttcgagcgca taccgcaatg ccggcgaatt gccgccgggg
    13321 gccgtacttg tcgtggggag cggcgcgtcg ggctttcaga tcgccgagga tttgcaccaa
    13381 aatggccgac aggtctccgt ctgcatgggg cgccatcgtc gccttccgcg acgctaccgc
    13441 gggcgcgact acgcctggtg ggcgatgaaa ttgggcgtat tcgaccgtcg gcgcgcagag
    13501 atgtcggcga tctctcagcc gttcgcgttg ttgaccggcg tcaatggcgg ctacgaggcc
    13561 gactggcggg tgttggcttc aaaagggatc gttttgttgg gtcggttaac agccgtcaag
    13621 ggaactaaac tcgctttggc tcccgacttg gaagagaacc tcgccaaagg agacgagtgg
    13681 ctcgccaact tcaagaaatt ggtggatgac tacgtcgcca aaaacaaatt ggatttccca
    13741 acggactcat ccccccgtct ggaaggcggc gcatcggcgc cgattctgga attggatctc
    13801 agctccgcgg gaattacttc gatcatctgg gcaacggggt tctgcaacga ctacgaatgg
    13861 ttaaaactac cgcttctcga cgaccgccgg gaacctgtgc acgagcgtgg cgtcacgcaa
    13921 tttccgggaa tctattttct cggcttgcgc tggctctaca aacagaaatc aggctttcta
    13981 tcttttggcg gacctgcgga agacgcggcg tatctggcgg agcagatcgc cgcgagacac
    14041 aatggaagac cgagccggtc ttcttgattc tggattcggc aggttgtggc gacgaaaatc
    14101 aactcccaat gaaaagcaaa tctccggctg gatgttatgg cgagcaaaaa aggctctaag
    14161 agcaaaacag caaaaccgcc ccgcagcggc gtgaaaacgt ctccggttgc aaaggtacaa
    14221 actatggcgg agctgcgccg ccagctcgcc gactcagcca aagaggcgca g
//
LOCUS       DASUVS010000436        23941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1941933, whole genome shotgun sequence.
ACCESSION   DASUVS010000436 DASUVS010000000
VERSION     DASUVS010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 23941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23941
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1941933"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VF182_22950"
     CDS             <1..507
                     /locus_tag="VF182_22950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439995.1"
                     /translation="ANVKLVSGFKGTAEIRLAMKSGEVDSVITGWETLRVTNLQDFDS
                     GEWLILSQWVDEPLTDLPQKNVPLIYQFARNDEERQLFKLGLIKPNNYARPYVLPPGV
                     PQDRVKAIEAAFQKALRDPELLAEAERSKLNINPISGAELRSMILEGLSMPKSIKEKL
                     RPMLAPAS"
     gene            complement(602..1291)
                     /locus_tag="VF182_22955"
     CDS             complement(602..1291)
                     /locus_tag="VF182_22955"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX6439996.1"
                     /translation="MIRLRLFHLRQCPLIFLALGCVISLALAASGAPGLTARESGVIL
                     YSGHDTDSLQLTTLQKDETLTFVAEAVGNERWYMVRTRQGVIGWLRASDAILSESAKR
                     DYSQELLSTSSWTAHATQGRTYVGTWSVGAQSSTNLAGGTWTLQDAAGTTVLTGSWSA
                     EKFSTGWNGVWRAHSEEYKAEYSGSWSADIPHDSESRLPNLFETAARNAIRGVWTGGT
                     QSGTWSIRAAN"
     gene            1473..1934
                     /locus_tag="VF182_22960"
     CDS             1473..1934
                     /locus_tag="VF182_22960"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6439997.1"
                     /translation="MADGVMRDGNKTKTERKEMDKDRAALNLKVVENHFHSEALNEVE
                     AALEMFTDDIVWEAPAPNGLNRSFSGKEAVAKNYRELFASMREVRFQFLQRFATEDRV
                     VDDSMVTFEVARDGYWHFPLGSKVEMRLTHIFEMRDGKIAREIVFDMGRAV"
     gene            complement(2285..2524)
                     /locus_tag="VF182_22965"
     CDS             complement(2285..2524)
                     /locus_tag="VF182_22965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6439998.1"
                     /translation="MIATASNGEVEHEDSQREDITPNVVSHLDLDNQRKLLLIRAFFQ
                     HWLLKPFQKFAGMKGWKIYAGFQSGTMIYLHYVLQ"
     gene            2658..3014
                     /locus_tag="VF182_22970"
     CDS             2658..3014
                     /locus_tag="VF182_22970"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6439999.1"
                     /translation="MSRIRHIAIVTSNQEKLGNFYKTAFGMKEVKGIGSALYLSDGYM
                     NLALIKKTPEVKADEGLYHLGFEVDDVEPLKEVFKQVGASSEVQPRPKNRDAEFRVHD
                     PDGNAIDLVCSRKWPV"
     gene            3174..3599
                     /locus_tag="VF182_22975"
     CDS             3174..3599
                     /locus_tag="VF182_22975"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6440000.1"
                     /translation="MGVEIIDLVELARQEKRRKVVFNTPRCHAWVHYYPNPGERDDMH
                     CHNADQTFYVIDGECTMHFPDGGKAVMKPGMVATITGGSFYQLENSGDGPMIMMGNRS
                     GPSEAIQHINYELRKDIKKLSKEELERANIPHISTQGRN"
     gene            3638..4687
                     /locus_tag="VF182_22980"
     CDS             3638..4687
                     /locus_tag="VF182_22980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440001.1"
                     /translation="MKAMAMAWFGMFLGLIPNLSWAQPDFYRGKLITLIQDSTPGGVG
                     QLRTQALLPVLQKHIPGEPRIVMQFMPGAGGRTAANHLYNNSARDGLTIGRVSSGLVT
                     SAILGLPGVQYDINKLIYLGSGHNEVNNVFFSRSQLGLDNLNKLKAATGLRIGGQSVG
                     HSNYVIARLFAWLLDLKEPRFVVGFSGAEMDVALLQGELDSRANSAETVVQRTPQFIN
                     ERLMNFHGVAEIPHGYRYKHPAFSDLPALHTFGKTDKEKRVLEMFAAFGRFSQAFVLP
                     PGTPNDRVKTLKDAFRKSWKDPEFHESWKKMTRADPSPLMPEELEELVKNIPRDPEDV
                     KLYNRIAGAEPLPKR"
     gene            4737..5153
                     /locus_tag="VF182_22985"
     CDS             4737..5153
                     /locus_tag="VF182_22985"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6440002.1"
                     /translation="MIKLIDQMKDLINNALENGCPCVLATAAPDGEPDIGFKGSMMVF
                     DDETLAYWERTKRQHLKNITANPKVVVLFRDGKSRAAWRFHGLAEVHEAGAIRDQVMA
                     RTVPAELEKDPDRKGAAVVIKLDKVTNMAGQVLQSR"
     gene            5809..6030
                     /locus_tag="VF182_22990"
     CDS             5809..6030
                     /locus_tag="VF182_22990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440003.1"
                     /translation="MNQVAKRIFALGILTMLATGVAACDYNRDYYNDSYRDRDRYDRY
                     SDRDRDARYDRDRSWDWRNRDRRDWDRNR"
     gene            complement(6126..7136)
                     /locus_tag="VF182_22995"
     CDS             complement(6126..7136)
                     /locus_tag="VF182_22995"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HEX6440004.1"
                     /translation="MSAHTVSTPTHERCVTSYAARTQYKPGEVRPPHIKVKDAIDIHC
                     HAHEGQQNPYDLARRASLAEMKGILFKSIPGDDPAKAVRDVQEDVNRWAEKERVEPVR
                     CWSAYVCGRQMAPVTAEIVSEMIDRGVSAIWLPVFTHAITLSTVGTYQRFIDGNSAAG
                     WHGPVPWDKALAIGHYNLDDKGELKPAVKEIVRICADRNVALFFGHATHKEIYKLAEE
                     VEKVGLQRAVIDHPYSPFLNVSPEMMKELSPVGILFNFTWDELSPLLGVDPQIMYNTI
                     REIGPEHFTLSSDAGEPLFPDSVEAMRLVRGYMEAFGLNQDELHTVCTKNPAKVVGLA
                     LG"
     gene            7298..8293
                     /locus_tag="VF182_23000"
     CDS             7298..8293
                     /locus_tag="VF182_23000"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440005.1"
                     /translation="MKLRWIGLVYAIAAVVMDGATALAADKLPFIYTARVMSQSYPWI
                     AQEAGLFKKYDLEVPLIFVTAGAPAVATILAGDSEVTQQGAAALSRAFVQGNRDVVFI
                     GGIKSILTHSILAKPDIKTPADLRGKRIGVSRIGSNPHYFAVQALRHFGLESRDVSYV
                     QAGGAPETLAALLAQGIDAAVLTVPTDAQALKLGYHYVVYGPDLKIAYAATTFNTRRS
                     IMAKRGPVIGRFMRAMAEAAKIMHTDKEFTYKIMEKYLLVNDRKLLEASYNVEIKALE
                     PRLALKLEGFQSTLDEIAPIDPRAKTVKPQDMVDTRYLDEMEKSGFFDQIWAGKR"
     gene            complement(8598..9587)
                     /locus_tag="VF182_23005"
     CDS             complement(8598..9587)
                     /locus_tag="VF182_23005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HEX6440006.1"
                     /translation="MKISKKMTLMAFGLFAVIGLSPIALETYADARAGGGRSGGFRGS
                     RSYQAPSRPAQPAQQRRDATPPPQQPGQFAPQSGGFMRGLGTAVLGGFLGSMLFSGLA
                     NAGGFGGIGGLGGSGFGLFEILLIAGIGYFLYRKFRSPAAASGYGTMPYQNTNYQANT
                     DYQPSYGYRAPESGRLQEMPSGNEVDYRSLTLMDRSFDPNQFLKTAQDTFFKVQGAWN
                     KQDTATLNRLCGAELMQSWQEELSRLSARGQQNRMENIALSSSEITEAWTESGQDFIT
                     VRLNANLLDYTVDRSSGAVVSGSADEPIQFEEYWTFSRPVGPNAWKLTAVQQA"
     gene            9899..10963
                     /locus_tag="VF182_23010"
     CDS             9899..10963
                     /locus_tag="VF182_23010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440007.1"
                     /translation="MRGPIQFSSFFTLLLGFVSMPLFPNTVYSQAPYYQGKTITILRG
                     GEPGGSGDMQARALIPFLKKYIPGNPTIIVENVPGAAGMKAVNQVYSSAKPDGLTIAA
                     VGSGLASGAILGLPGARYDIDKLIYLGSTEHGDPYIFLSRKEAGFDSLDKLRAAPSVR
                     LGAQTVGHAIYVSGRIFAYVLGLKDPRMVVGFGGPELDTALVRGEIDARANSADTVVR
                     RNAEALAKGEYNVHATLTIPKGKFHPRFANVPDADSLATNDRERQLVNLFRAFMYPRW
                     PYVLPPGTPAATVKILRGAMASAFKDPGFSGEFQKLMGGEPSPLTGEDVETSIRELPR
                     DPEVIGLYKKMAEHGPLPSR"
     gene            complement(11176..12225)
                     /locus_tag="VF182_23015"
     CDS             complement(11176..12225)
                     /locus_tag="VF182_23015"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440008.1"
                     /translation="MSTLDQPLLIANVNYHVGHWVAIYVAEEQGFFKQEGLSRYEYER
                     SGLLPGPSESQTLGLAIKEHGVDIATAVDTESAIIQRVQGADVYIVGGWRYTPFLKWY
                     GSKHITDMSKLRGGKIGVRENSADGGGLVEFFIEEALKDAGVDPATEVEMVHDPVFGY
                     RNNPAHIDMLRSGKVNAITSSPPFSDDLEKEGYPVILDPNVVFPRRPGKVTVAPHRTI
                     EQRSEELKAYFRAITRTFWFMREVNNFQYLHDLEARLRKQTHNDDEQRLFIASAPDRV
                     DSWALPMDGGIERHALERIIQEMVKRGRLERPISVGEVFKDDAVQDAYRDISTRGELK
                     PAFNKAMAVLERYGF"
     gene            complement(12222..13214)
                     /locus_tag="VF182_23020"
     CDS             complement(12222..13214)
                     /locus_tag="VF182_23020"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440009.1"
                     /translation="MKLSPSPRYSRSIFLLFLTIGLAPARPSLALDEAKIHVAGTPNI
                     TSFVYHVGKEKGFYREEGIELQPIMAGMLQGIQGLIGGSFDYSQMLGQGGGAIMRGAP
                     LKIIMAFDTRPSWFLLGTKNMKRVQDLKGKQLAVSSFGAALDQMTRELLPRFGMEPMR
                     DVTLRAIEPPPNRLAALLSGAVDAAVLTHPETIVARRQGFKELLFFGDHIEFVSAGVV
                     ATERNISQRPDFVRRFIRGALKTFYWLKANEKEVAARLAKATKSSEDDGLELYRTGVK
                     LFSEDGAIPRPLQERMISLQRKVLNVEKDVPPENVYDFSFVRAGNKELGKGASS"
     gene            13460..14431
                     /locus_tag="VF182_23025"
     CDS             13460..14431
                     /locus_tag="VF182_23025"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440010.1"
                     /translation="MLSVLIFVLLIFSSASEAISAESQKLDRVRIGYSSISSSRIALW
                     VASDMGFFKKNGLAPEAIVTPGVQGTQALIAGELQFYLGGVDSAALAAARGSDLVVLA
                     TAEPIEYKLITQPNVKTVKEVRGKKIVVDRVGGTSYYISLQILEKVGLKSGEVELVQV
                     GGGGNQRVAAFKSGLVAGVVTSPDRFEQLKIPYHVLADAMELGIKVMGNSYLTTRSFR
                     DQNKDVVLRTVRALVQGRRWAKDPKNRQEVLRIYNRYLPSPDSAFMDHLYRKNVESMP
                     LYPYTNIEDLRIFLSYLSDANPNLRNVKLAEFVDNSYLKRIEQESGS"
     gene            14908..15945
                     /locus_tag="VF182_23030"
     CDS             14908..15945
                     /locus_tag="VF182_23030"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440011.1"
                     /translation="MSTTDQCLLIANSNYHVGQQVAVYVAQEQGYFQEEGLEKFDYDG
                     RGLIPAILERQGLGPAMVEHGVDIVTAVDVSAAIYQRSLGADVYVVGGWRYDPDLKWY
                     SRKDITDIRQLKGRRLGIREREGLVYGFMASLLVQAGIDPEKEVTWIFDPVFGYGNDP
                     RHADELRQGKVDAMPSFPPFSKQLESEGFPILMDPRIVFPRRPGKVTVATKRTIDSRA
                     EELRAYLRAMIRAFWFMRDTENFDYLRDLESRLRSQSHNDEERQLFIVTSVEKVDGWA
                     LPIDGGVSAESLNRIIQEMVAIGNLKEPINVQSLLHDSAVREAYQELVKRPELRTAHD
                     KVKLVVEKYGF"
     gene            complement(16014..16847)
                     /locus_tag="VF182_23035"
     CDS             complement(16014..16847)
                     /locus_tag="VF182_23035"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440012.1"
                     /translation="MGRIVLGLAAPHNPNITSRPDKMSPEVQNKLDSAFGHLKKGLSE
                     ASPDCLLILTSDHVTNFFYNNAPMFCLGVSDTCTGPAPKEMAELRIPRATLKVDTTMA
                     KGLLNYGIENGIDFSYSQEMILDHAFMVPLSLLTPRMDLPIVPLHINGMLPPRPTARR
                     CFRLGQQIGDYLRNHYNGNVAVLASGSFSGDIGGPKMGSIDTALDLEFLDLVKDAKAQ
                     EMVDKATAETLEHAGVANEILIWITLQGVLGKLKPSFTDYICGKDWSTAATLATWQPR
                     L"
     gene            complement(16851..17102)
                     /locus_tag="VF182_23040"
     CDS             complement(16851..17102)
                     /locus_tag="VF182_23040"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440013.1"
                     /translation="MSLYQVDKLLFTLFNDLELQEEYKQNPEAVLKRYDLEDFEAKAL
                     REVNAGELYRMGAHSFLLWQFSRLMDLKPAIYFKQVRGE"
     gene            complement(17099..18100)
                     /locus_tag="VF182_23045"
     CDS             complement(17099..18100)
                     /locus_tag="VF182_23045"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440014.1"
                     /translation="MTTRAKSIFRNPARNLLLISCLACTLGLARASFALDEVNLHISA
                     GISGNALLYKFTIEKGYYREEGLDVRAIQAGMLPGIQGLVGGSFHFSQILGQGAGAIL
                     RGVPLKIVMVFDTRPLNWLFARKEIKTVQDLKGGKQIAVSSFGAALDQMTRDLLPRRG
                     IDPQKDVVLRAIEPTPNRLAALMSGAVDAAVVNQMDRIIAKKHGLNELLFYGDDLEFV
                     TAGAVTTERTLAQRPELVQRFLRGTLKGFYWLKNNEKDVVARMVPIMKVSEAEATDVY
                     RAWLRVMSADGTIPRGLQEKMIAFQRKSLKVERDVGPEDVYDLSIVRSVNEEMRKSK"
     gene            complement(18284..19327)
                     /locus_tag="VF182_23050"
     CDS             complement(18284..19327)
                     /locus_tag="VF182_23050"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440015.1"
                     /translation="MSTLDQPILIANSNYHVGHQIAIFVAEEQGFFREEGLKEYEYDA
                     RGLIPGPLERDGLAMAITNHGVDIATAVDVEAAIYQRSLGADVYIAGGWRYTPFLRWY
                     GSKHITDMSKLRGGRIGMREKEGLVQVFITNALQEAGVDPEKEVEWVYDPVFGYRNNP
                     AHMEMLRSGKIDAITSQPPFSEQLEREGYPMILDPNTVFPRRPGKLTVATGQMIENRG
                     DELRAYFRAIIRAFWFMRDVKNFEYLRDLEKRLRKTNTHNEDERGVVAIVTAPDRVES
                     WAIPIDGGIASDAVQRIADEMVKSGKLSHSMSARDIVRDDAVKQALKEVSSRTELKTA
                     FRIALAAQEKYGF"
     gene            complement(19424..20374)
                     /locus_tag="VF182_23055"
     CDS             complement(19424..20374)
                     /locus_tag="VF182_23055"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440016.1"
                     /translation="MPDTHLTLSCGDYDRTRPLIDRSVPTPGLDLTVIPLPSAERHTR
                     FVRNLEFDVCELQIAQYLGLKSRGFPITAIPVFPHRRFNHSCVMVRIDSNILRPEDLR
                     GRRVGVHGHFNPIALWIRGLLQHEFDLPSSEIHWIADGSEDVPGWSPPSWLRIDHAPA
                     GRKMQDLLKAGELDAQILSDSGADASAINKIVRRLWPNYREVEVDYYRRTGIFPIRHL
                     VVVKDEVLQRGGEVASQLVRAFEDAKQQAYRYWADHRRSSLAWFGAEQEEERALLGPD
                     PWPYSVEQNRVVLETLLDYAFEQRLTERRLDIQEIFAPGT"
     gene            complement(20526..21512)
                     /locus_tag="VF182_23060"
     CDS             complement(20526..21512)
                     /locus_tag="VF182_23060"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440017.1"
                     /translation="MKRSYQLFGLILSMQMFVFVSASFAAGETQRLKIIYSSFTGGYT
                     PLWIAVDERLGRKYGLDLEAVYAGRARPHQLLLSGDAQYVISTGTGVVSSYAVGQKDL
                     VIIATFLNVTGSSLFSKPQINKPADLRGKVVGSGRPGAITDVMLHYILKNKLNLDPAR
                     DVKIVPLGDGPSILPALEKGVVDAAVLTTPTRLLAKRMGFREMLDFDEMGIPYPYVGV
                     STLKVNVKKNPEITARLVRTLMEAIQIFKTNKETSMAVMRKYLRGANDDILAETYSYF
                     SAKMQKSPYPTADAVMTALDMMSVQFPQAKTVDPNEIIDTSFVKQAESSLSQ"
     gene            complement(21694..22659)
                     /locus_tag="VF182_23065"
     CDS             complement(21694..22659)
                     /locus_tag="VF182_23065"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440018.1"
                     /translation="MVGSFPWLLPRIVMAEDVIQIGLFNKDPAIIAADAKGFFNKESI
                     RIQIDTVTDSPTLLRNLISGKYDLIMNNADNVIAWAEGQGEDPQKNDFVIFLGGSRGV
                     NQKLIVGSGINDYGDLKGTIFAVDAPTTGYAIVGVYILKKHGLELSKDYMFKAFGNTT
                     KRAEAMSRGEASAAMMSLADDEIQKRGFKVLAKAEDYVQHYARGLGATRRDWANKNED
                     LLVRFIRAMIRATDWVTESNHKDEVIAMLMPENRNNKARAQAMYEESISPRFGFSPRS
                     QIDMDGIQSVLQLREIAGLMKPPLPKPEKYVDERFYKKALASLDR"
     gene            complement(22909..>23941)
                     /locus_tag="VF182_23070"
     CDS             complement(22909..>23941)
                     /locus_tag="VF182_23070"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440019.1"
                     /translation="SEYRRSQILIICVYSGREIKGRNHLKHITCGFLLLASVFYSVSN
                     PALSTAEEVTLSYTASTLTFLPGRVAVDQGFFRQEGLDVKFIQMRTAALIPALANGHI
                     DYTMSFLPPIDSALRGVPMQMLAVFVDRSLHYLVTRPTVKSDADLRGKTFVLNTVDLS
                     GSTGLVFQAVLRHFGLDPLKDVKWIPAPDSPALFAMVEQGVADATFLVPPWPQKAREA
                     GLSVLFRAGDIYSAPLAGLSTHRRTIKENPNQVRRMLRALVRGSRFILQPSNEQAVGA
                     SIQQWLRMPAQEGIAALKDVTFAYSDGAPRNDKSFWDVVATRAQLLSSKVALSEVADF
                     SMVKEIRSK"
BASE COUNT         5392 a         6364 c         6577 g         5608 t
ORIGIN      
        1 gctaatgtta aactggttag cggctttaag ggaacggcgg agattcgttt ggcgatgaaa
       61 tccggcgagg tcgatagcgt gatcaccggt tgggagaccc tgcgcgttac caatttacaa
      121 gactttgaca gtggtgagtg gttgattctg tcacaatggg tggatgaacc gctgactgat
      181 ctgccgcaga agaatgtgcc gctgatctac caattcgcgc gcaatgacga ggagcgccag
      241 ctgtttaagc tcggcctcat caagcccaat aactatgccc ggccttatgt cttaccgccg
      301 ggagtgccgc aggatcgtgt gaaggctatc gaggcggcgt ttcagaaagc gttgcgcgat
      361 ccggaactgc ttgcggaggc cgagcgatcg aagctgaaca tcaacccaat ctccggcgcc
      421 gaactgcgta gcatgatctt ggaagggcta tcgatgccta aaagcatcaa ggagaagctc
      481 cggcccatgc tggcgccggc cagctgaggc tcgatgaccg ggtttataaa tgaccagtcc
      541 cgagtcttga ccttcactgc cagtcatttc ggaattgcac gtaagttgct actccacagc
      601 atcagtttgc cgctcggatc gaccacgttc cggattgagt tccgccggtc cagactccgc
      661 gaatggcgtt gcgcgcagca gtttcgaaca agttagggag gcgtgattct gaatcgtgcg
      721 gaatatccgc ggaccaactg ccgctgtatt ccgccttgta ttcctcgctg tgagcgcgcc
      781 atacgccatt ccatccggtc gaaaattttt ctgccgacca gctgccggtt aggaccgttg
      841 ttccggcggc atcctgcaat gtccatgtgc caccggccag gttagttgac gattgcgctc
      901 ccacagacca agtgcctacg tacgtccggc cttgcgtcgc atgggcagtc caactcgacg
      961 tagagagaag ctcttgcgaa taatctcttt ttgccgactc gctgaggatt gcgtcagagg
     1021 cgcgcagcca gccgatcacg ccttggcgcg ttcgcaccat gtaccacctt tcgtttccca
     1081 ccgcttcggc aacgaacgtc agcgtctcat ctttttgtag tgttgtgagt tgaagagaat
     1141 ctgtatcgtg ccctgaatac aaaattacac ctgattcgcg tgccgtaagg ccgggtgccc
     1201 cactcgccgc cagagcaaga gatatcacgc agccgagcgc gagaaatatt aacgggcatt
     1261 ggcgtaaatg gaagagacgg aggcggatca tggattctcc tgacccgaat tgtaagcttt
     1321 tatcgccgca gcggaaagct cggatgtcgg gagtgactcg taccttggca gtcgagtggt
     1381 gtaattttct ttgatccatg cgataaagtt tttattttgg gtttgacagt ggccccatac
     1441 ggctctcggg gagcggtttc agtggcgggg gcttggctga tggagttatg agagacggca
     1501 acaagaccaa gacggaaagg aaagagatgg ataaagatcg cgcggcgctg aatttgaagg
     1561 ttgtggagaa ccattttcat agcgaagcac tcaatgaggt cgaagcggcg ctggagatgt
     1621 ttaccgacga tatcgtttgg gaggccccgg cgccaaacgg cctaaatcgg tcgttttccg
     1681 gaaaagaagc ggtcgccaag aattatcggg agttattcgc gtccatgcgt gaggtgcgat
     1741 ttcagtttct tcaacgtttt gccaccgagg atcgtgtcgt cgatgatagc atggtcacgt
     1801 tcgaggttgc gcgggatggg tactggcatt ttcctctcgg cagcaaagtt gaaatgcgac
     1861 tgactcatat cttcgagatg cgagacggga agattgcacg ggagatcgtc tttgacatgg
     1921 ggcgcgctgt ttagtcttcg atccaatcgt cagcattggg aaatggcgac gcgcacgtga
     1981 ggggagttca gaaccgccag ttcgagaaca acactgatcg gcattgtctc tccccattac
     2041 cggccaattc ttcctgatcg gagtggaggg gcaagcgcgg gtcctgagca acatattgat
     2101 ccggtgagat aagattgcag aaaaggaagg gttcagatgc ttagccctgt ctttctgagg
     2161 gtgaaacgcg tgcactgaaa gaaatttatg ccccgcgtca accgccgttt tctgcacgga
     2221 cacgccttcc actgctacgg tgtttagcac gacgagaaag taactcagtc ccggtggcca
     2281 atcattactg cagaacataa tgcagataga tcatcgtgcc actctggaac ccggcgtaga
     2341 tcttccatcc tttcatgccg gcgaatttct gaaaaggttt caatagccaa tgttgaaaga
     2401 aagctcgaat aagtagaagc tttctttgat tgtccaaatc caaatgactg acaacgttcg
     2461 gtgtaatatc ttctcgttga gaatcttcat gctcgacttc tccatttgac gctgtagcga
     2521 tcatatggcc acgtagtcgc tacacccgcg tagccctgcc ttgacgaatc gtcccgatca
     2581 gcgatatagc aagtgcgcca cttgccctct tgccgcgatt taaggagacg cgaatcgaca
     2641 gaaataagga ggaaaatatg agccggatcc gtcacattgc aatcgttacg tcgaaccagg
     2701 aaaagctcgg aaacttttac aaaaccgcct tcgggatgaa ggaggtgaag ggtattgggt
     2761 cagcgcttta cctttctgac ggatatatga acctcgctct gattaagaag acgccagaag
     2821 tgaaggctga cgaggggctc taccatttgg gattcgaggt agacgatgtc gagccgctta
     2881 aagaggtttt caaacaggtt ggagcctcaa gtgaagtgca acctagacca aaaaatcgtg
     2941 acgctgaatt tagggttcac gatccagatg gaaacgccat cgatcttgta tgctccagga
     3001 agtggcctgt atagaagggt cgcagtcgat gaacgatgac tgcacacggc ctgcgcgtta
     3061 ggcgtgggct gatctggcca aagggctgcg gtggttttgc tttagaggcg agctcgcagc
     3121 agggatgagc cttagaaccg agacaatatg aactgaacgt aaggaggatt gacatgggcg
     3181 ttgaaatcat tgacctggtt gaactcgcac gccaggaaaa gcgtcgcaaa gttgtgttca
     3241 atacaccccg ttgccatgca tgggtccact actatccgaa ccccggcgag agggacgaca
     3301 tgcattgtca caacgcggat cagacatttt atgtcatcga cggcgaatgc acgatgcatt
     3361 tccctgatgg cggcaaggcg gtaatgaagc cgggaatggt tgccaccatt accggtgggt
     3421 ccttctacca attggaaaac agcggtgatg gcccaatgat aatgatgggc aatcgctccg
     3481 gaccttctga agcaatccaa cacatcaact atgagcttcg aaaggacatt aaaaaactca
     3541 gcaaagaaga attagagcgc gccaatattc ctcatatttc gacgcaggga agaaattaat
     3601 taatcccgct cgatgtcaga tgttccggag gtgtcccatg aaagcaatgg ctatggcttg
     3661 gtttgggatg ttcctcgggc ttatcccgaa tctatcatgg gcccagccag atttttatcg
     3721 cggcaaactt attacgctca tccaagacag cacgccgggt ggggtgggcc agctccggac
     3781 ccaggcatta cttcctgttc tgcagaagca cattcccggt gaaccaagga ttgtaatgca
     3841 gttcatgcca ggcgccggcg gcagaacggc cgccaatcat ctttacaaca actcggcgcg
     3901 tgacggctta accattggcc gagtgagcag tgggctcgtc acctctgcta ttctcggtct
     3961 gccgggcgtt caatacgaca tcaataagct tatctacctc ggctctggtc acaatgaggt
     4021 caacaacgtc tttttttcac gcagtcaact cggtctcgat aacctcaata aactcaaagc
     4081 agctacaggc ttgaggatcg gcggacagtc tgtaggccac tccaactatg tgatcgctag
     4141 actttttgcg tggctgcttg atttaaaaga acccaggttt gtggtgggct tttccggagc
     4201 tgaaatggat gtggctctcc tccagggcga gctagattct cgcgcgaaca gcgcagaaac
     4261 tgtggttcaa cgtactcctc aattcattaa cgagcggctt atgaacttcc atggtgtggc
     4321 tgagatcccg cacggatatc gctacaagca tcccgccttt agcgatctcc ccgcattgca
     4381 tactttcggc aagactgaca aggagaaaag agtgctggaa atgttcgctg ccttcgggcg
     4441 gttcagccag gcttttgttc taccgccagg cactcctaat gaccgagtga agactctcaa
     4501 agacgctttc aggaagtcat ggaaggaccc tgaatttcat gaaagttgga aaaagatgac
     4561 tcgtgcagat ccttcccctc ttatgcccga ggagttggag gagctggtta agaatattcc
     4621 aagagatccg gaagacgtta agctttacaa taggatcgct ggagcagagc ctttgccgaa
     4681 gcgataaagg cgccatgtct gttgacatcc gccttcggct tttctagatt cggcccatga
     4741 ttaaattaat cgatcagatg aaggatttga tcaacaacgc cttggagaat ggatgtcctt
     4801 gtgttctcgc cacggctgcg ccggacggcg agcctgatat cggtttcaaa ggcagcatga
     4861 tggtctttga tgacgaaacc ctggcatatt gggagcgcac caagcggcag cacctgaaga
     4921 acatcacggc gaatccaaaa gtggtcgtgt tgttccgcga cggcaaatcg agagctgcct
     4981 ggcgttttca cggactcgcc gaagttcacg aagccggagc aattagagac caagtgatgg
     5041 ctcggactgt gcccgcggag ttggagaagg acccggatcg caaaggcgcc gcggttgtca
     5101 tcaagctcga caaggtcact aatatggccg ggcaggttct acaaagccgc tagcttgcgc
     5161 gggcgctcct ctgttcgtca acgctccgac gtctctgcgc ccgatcgctc gagaaatcag
     5221 cacccgccca tttcttcgcc gatatcttgt tcatgtggca ggcagctcgc ttgagattaa
     5281 gtaaaatcga gcgttttcat cttcattaag cgtcggaagc cgatcgactg ctcgaggaga
     5341 gcgggctgga actggcattc tgacatggat taatcccgga tccgcagttc aaaaaaggca
     5401 cgcctaatca agcggcgagc cctccagagc gaatgcaatg ggccagtgag ttggagcgtc
     5461 ctcgaaatcg gcggtggtgc agcggactca ctggaaagga ttgaccccct ggaccaagca
     5521 ccgtagagag cattgaaatt attcgcttct ctgccttcgc tcctccggac tcgccccttg
     5581 atcagtacac atcgttaatc tctactgcgg attcgagtta atctgtaagg agaatgtccc
     5641 atttctttct tagctgcgat ttcctccgta atctgttcca gacctgcatc tcgcatcgag
     5701 gaatagcgac ctgccaattt gtcgaaaacg ggattgtgtt aacatgtaga tgccctgatc
     5761 gtccaaagag cgagtggtat agcttgcgca ctcagatcag gaggaatcat gaatcaggta
     5821 gcaaaaagaa tatttgctct cgggatttta acgatgttgg caactggcgt ggcggcatgt
     5881 gattacaata gagattatta caacgattct tatcgagatc gcgaccgcta cgatcgttac
     5941 tcggatcggg accgtgatgc gcgctatgat agggatcgaa gttgggattg gcgaaatcgc
     6001 gatcgccgcg attgggaccg taaccgatag cggattaaac cggtagcctg cggcatgtcc
     6061 ggtgctctgc ttggaagtga aatattaact tagaattttc agccgcgact gcattggcgc
     6121 tcggtttagc cgagcgccag tcccaccacc tttgccggat tcttggtgca tacggtatga
     6181 agctcgtcct ggtttaagcc gaatgcttcc atgtagcccc gcaccaggcg catcgcttcc
     6241 accgaatcag gaaagagagg ttcgccggcg tcgctcgaga gcgtgaaatg ctccgggccg
     6301 atttcgcgga tcgtgttgta catgatctgc gggtcgacgc ctagcagtgg cgatagctcg
     6361 tcccaggtaa aattgaacaa gatgccgacg ggcgaaagct ctttcatcat ctccggcgag
     6421 acgttaagaa acggactata agggtgatct atgacggcgc gctggagccc gactttttcg
     6481 acttcctcgg cgagcttata gatttccttg tgcgttgcgt ggccgaagaa aagcgccacg
     6541 ttgcgatcgg cgcagatgcg aacgatctct ttgacggcgg gcttcagttc gcctttgtca
     6601 tccaaattgt agtggccgat ggccaatgct ttgtcccacg gaaccggtcc gtgccaaccg
     6661 gctgcgctat tgccgtcgat aaagcgctgg taggtgccaa ccgtactcag ggtgatcgcg
     6721 tgggtgaaca cgggtagcca gatcgcgctc acgccccggt caatcatttc gctgacgatc
     6781 tctgcagtca ccggcgccat ttgacggccg cacacatagg ccgaccagca tcgaaccggc
     6841 tcgactcgtt ccttttccgc ccatcggttg acgtcttcct ggacatcacg aacagctttt
     6901 gccggatcat cacccggaat gctcttgaat aaaatcccct tcatctccgc gagcgatgcg
     6961 cgccgagcca aatcataggg attctgctgg ccctcgtgcg catggcaatg gatgtcgatg
     7021 gcatctttga ctttgatatg cggcggccga acttcgccgg gcttgtattg cgtgcgcgcg
     7081 gcgtaagatg tcacgcagcg ttcatgagtg ggagtagaga cggtatgtgc gctcatattt
     7141 cttctccaag cttgcctgag ttgtatcaat gaccgggtcg tttgcaaagc gaaattcatc
     7201 aaagacatct gcgatctgct tgaccctgga cctccaggta gctattttcg cgccatcaaa
     7261 cgcaagctct gacgagaagc ccgcaggagg ataaaacatg aaattgcgat ggattgggtt
     7321 agtttatgca attgccgctg tcgtgatgga cggcgcaact gcgcttgccg cggacaagct
     7381 gccgttcatc tatacggctc gcgtcatgtc gcaatcttat ccctggatcg cgcaagaagc
     7441 cggattgttc aaaaagtacg atctggaagt gccgcttatc tttgttacgg ccggcgctcc
     7501 ggcggtggcc acaatactgg ccggcgatag cgaggtgacg cagcaaggag cggcggcttt
     7561 aagccgcgcc ttcgttcagg gtaatcggga tgtcgtcttt atcggcggca ttaaaagcat
     7621 cctgacccat agtattctcg ccaagcccga catcaagacg ccggcggatc tgaggggcaa
     7681 aagaatcggc gtgtcacgga tcggcagcaa cccccactac tttgcggttc aggcgttgcg
     7741 ccactttggt ttagagagcc gggacgtcag ttacgttcag gcgggcggcg cacccgagac
     7801 attggcggcg ctgcttgccc aggggatcga cgccgcagtc ttaaccgtgc ccaccgacgc
     7861 tcaggcgctc aagctcggtt atcattatgt ggtatacggt cccgatctga aaatcgccta
     7921 cgccgccacc accttcaaca cgcgccgctc gattatggcc aaacgcggtc cggtgatcgg
     7981 ccgtttcatg cgcgcgatgg ctgaggctgc caagatcatg cataccgata aagaatttac
     8041 ctacaaaata atggaaaaat acttgctcgt gaacgataga aaattactcg aagccagcta
     8101 caacgttgag attaaagcgt tggagccgcg gcttgcgctc aagctcgaag gctttcaatc
     8161 gactctggat gaaatcgcgc ccatcgatcc acgggctaaa accgtgaagc ctcaagatat
     8221 ggtcgatacg cgctacctcg atgaaatgga aaagagcgga ttcttcgatc agatctgggc
     8281 gggcaagagg tagatataga gcgaattccg taattcaagg taacgcagga atcgcctgcg
     8341 agccctccga gtgaagacaa tagatccctg gctgatagcg agactgcaag ctccggtgct
     8401 ttaacgaacg ccctgagaca attgccatcc caggaccaat ggcattgaaa ctattcgctt
     8461 ctatgccttc gctcctccag ggtcactttc gaccctcaga ccgtgtgcat ctctactgcg
     8521 gaatccggga taaaaaaaac agcgaccagg accgaagtcc gtggtcgctg tgacagctgt
     8581 tatttggatg gtgaatgcta ggcctgctgt accgccgtta acttccaagc attcgggccg
     8641 acggggcggc tgaaggtcca gtactcttcg aactggatcg gctcatcagc gctgccggag
     8701 actaccgcgc cgctgctgcg atcgacggtg taatcgagga gattggcatt gagccgaacc
     8761 gtgatgaaat cttgacccga ttcggtccac gcctcggtga tctcgctgga gctcagcgca
     8821 atgttttcca tgcggttctg ttggccgcgg gcgcttagcc ggctcaactc ttcttgccag
     8881 ctctgcataa gttcggcgcc gcaaagtctg ttcagagtcg cagtgtcctg cttgttccag
     8941 gcgccctgga ccttgaagaa agtatcctgt gcggttttga ggaattgatt cggatcgaag
     9001 ctgcggtcca tcagcgtcag ggatctgtaa tcgacctcat tcccggatgg catttcttgc
     9061 agccgcccgc tttccggcgc tctatagcca tagcttggct gataatcggt gttcgcttga
     9121 taattcgtgt tttggtatgg catcgtgccg tacccgctcg cggctgccgg actgcgaaat
     9181 ttgcggtaaa ggaaatagcc gatgcctgct atcagcagga tttcgaataa accaaagccg
     9241 ctgccgccca agccgccgat accgccgaag ccaccggcat tagccagacc tgaaaagagc
     9301 atcgagccga gaaacccgcc gagaaccgca gtgccaagcc cgcgcataaa gccaccggat
     9361 tgaggggcga actgtccagg ttgttgtggt ggtggggtgg catctcttcg ctgctgagcc
     9421 ggttgcgcag gacgcgacgg agcctggtaa cttcgtgaac cgcgaaatcc gccgctccgg
     9481 ccgccgccgg cgcgcgcgtc ggcataagtt tctaacgcaa tcggcgataa cccgataacc
     9541 gcgaaaaggc caaaggccat caaggtcatt tttttcgaga tcttcattgg tcttttctcc
     9601 tcatggcaag gctgggatca tgaggagtct agtcgaaagc cgcagcacaa gcaatgcacg
     9661 atctcaaaaa gcgcagctcc ggttgcgcag cggatcattc ttgccgaggt ttgtggaaga
     9721 agcggccggc tgctaaagga tacctcgctg gctgtaacct tgacggaagg tatctccggc
     9781 agtaacctcg ccatactttg cagtctcgag ggccaggctg gcagttcggg attcttttat
     9841 tataggtgct gccctaaatc gttcgtaact caacattatc caaatgggag gtcatcgcat
     9901 gcgaggcccg attcaatttt cttccttctt tacgcttctg ctgggatttg tatccatgcc
     9961 gttgtttccc aataccgttt attctcaggc gccgtattat caaggtaaga cgatcacgat
    10021 cctccgaggc ggcgaacctg gcggttccgg agatatgcag gcgcgcgcgt tgattccgtt
    10081 tcttaaaaaa tacattcctg gaaatccgac gattatcgta gaaaacgtac ccggcgccgc
    10141 aggcatgaag gccgttaatc aggtttattc atccgccaag ccggacgggc tcacgatcgc
    10201 cgctgtcggc tcgggtctcg cttccggcgc gattctgggt ttacccggcg cccggtacga
    10261 catcgacaag ctgatttatt taggatcgac cgaacatggc gatccctata ttttcctcag
    10321 ccgtaaagag gccggattcg attcattaga caagcttcgt gcagcgccgt cggtgcgtct
    10381 cggggcgcag accgtggggc atgcaatcta cgtgagcgga cgcatctttg cctatgtgct
    10441 gggtctcaaa gatcctcgaa tggtggtcgg cttcggcggc cctgagctcg acaccgcact
    10501 agtccggggg gagattgatg ccagagcaaa cagcgccgac accgtggtgc gccgtaatgc
    10561 cgaggctctc gccaaaggcg aatataatgt tcatgcgacc ttgacgattc cgaaaggcaa
    10621 gttccatccg cgtttcgcca atgttccgga tgccgattcg ctcgcaacga atgacagaga
    10681 gcgtcagttg gtcaatctat tccgcgcctt tatgtatccg cgctggccct atgtgctgcc
    10741 tccgggaaca ccggcggcca cggtgaaaat cttacgtgga gcgatggcta gcgccttcaa
    10801 ggatccaggg ttcagcggcg aatttcaaaa gcttatgggc ggcgagccat cgcctttgac
    10861 cggggaagac gtcgagacct cgattcgaga attaccgcgc gatcccgagg tgatcggtct
    10921 gtataagaag atggccgaac acggtccgct gccgtcgcga tgaggctcgc ggtcgaggcc
    10981 ccaccttccc gcaactcaaa gccttgcaac taatgcaaac gatgactcct gcaaaaccgg
    11041 acgactgacg cctgggtgta ttcgtggttg gttatttttg gcggccgcta ttgcaatcaa
    11101 ttctgagtga gaagggtaga gcgcgtaggg gcgggtttta aacccgcccc tacgttgctg
    11161 ctcatgcatt gattctcaga atccatatct ctcgagaacg gccatggctt tgttgaacgc
    11221 cggtttcagc tctccacggg tgctgatgtc gcgataggca tcttgcaccg cgtcatcctt
    11281 gaatacttcg cccacggaaa tcggacgttc caacctgcct ctcttgacca tttcctgaat
    11341 gattcgttcg agcgcatgac gctcgatgcc gccgtccatc ggcagcgccc aactgtcaac
    11401 ccggtccggc gctgaagcaa taaacaggcg ctgttcgtcg tcgttatgag tttgctttct
    11461 caaccgcgct tcgagatcgt gcagatactg aaaattattt acctcgcgca tgaaccagaa
    11521 tgtccgagtg atcgctcgga agtaagcctt cagctcctcg ctgcgctgct cgatggttct
    11581 atgcggagcc acggtgactt tgcccggccg tcgaggaaag accacgttgg gatccaggat
    11641 caccgggtaa ccttctttct ccaggtcatc cgagaagggg ggtgatgacg tgatcgcgtt
    11701 tacttttccg gagcggagca tatcgatatg ggccgggttg ttgcgatagc cgaatactgg
    11761 atcatggacc atttctactt ccgttgccgg atcgacgccg gcatctttca gcgcctcctc
    11821 gatgaaaaat tccaccaggc cgccgccgtc cgccgagttt tcgcgcacgc cgatcttgcc
    11881 gccgcgcagc ttgctcatat cggtaatatg cttggagcca taccatttga gaaacggcgt
    11941 gtagcgccag cctcccacga tgtagacgtc agcgccttgc acgcgctgga tgatcgctga
    12001 ttcggtgtct accgcggtag cgatatcgac cccgtgttcc ttgatcgcca atcccagcgt
    12061 ttggctttcc gaaggaccgg gcaggaggcc gctgcgctcg tactcgtagc gcgagagacc
    12121 ttcctgtttg aagaacccct gttcttcggc gacatagata gcgacccagt ggccgacgtg
    12181 atagttgaca ttggcgatta gaagcggctg atccagagtg ctcatgacga tgctcctttt
    12241 cctaattcct tgttcccggc gcggacaaag ctgaagtcgt agacgttttc cggcgggaca
    12301 tctttttcta cattcaaaac ttttctctgg agcgagatca ttctttcctg cagtggccgg
    12361 gggatggctc cgtcctcaga aaaaagtttg acccccgttc tgtagagctc caggccgtcg
    12421 tcctcggacg atttcgtcgc tttggccagc cgcgcggcga cttccttctc gttggcctta
    12481 agccagtaga acgttttcag ggcgccgcgg ataaaacggc ggacaaaatc aggtctttga
    12541 gaaatatttc tttcggttgc caccacgcct gccgaaacaa attcgatatg gtcgccaaag
    12601 aacagcaatt ccttgaagcc ctgcctgcgc gccacgatcg tttcaggatg tgtcagcacc
    12661 gccgcatcaa ccgccccgct caacagcgca gccaatctat tgggcggcgg ctcgatggcc
    12721 cgcagagtga catcgcgcat cggttccatg ccgaatcgcg gcagcagctc gcgcgtcatc
    12781 tggtcgagag ccgcgccgaa gctggatacc gcgagctgtt tgcccttcag atcctgaacc
    12841 cgtttcatgt ttttcgtccc tagaagaaac cacgatggac gagtgtcgaa ggccatgatg
    12901 atcttaagcg gcgcgcccct cataatcgcg cctccacctt gcccgagcat ctggctgtag
    12961 tcgaagctgc caccgatcaa gccctgaatc ccctggagca tcccggccat gatcggttgc
    13021 agctcaatac cttcttctct gtaaaagcct ttctctttgc cgacatgata gacgaagctg
    13081 gtgatgttcg gcgttccagc tacgtggatc tttgcttcat ccaaagctaa tgatggtcgg
    13141 gcgggagcca agccaatggt caaaaacaaa agaaagatac ttcgagagta tcttgggctt
    13201 ggactaagct tcatcaagtc ctccgtggtt gcaaattact ggagtcacct ttttgttgaa
    13261 cgactcgatc actgttggat gacatgggcc ggtatcggca ataatgttta ctgcttagct
    13321 caacggccgt caaacagttt ggcgaatttt accgacgttt cgaattgcga gacagtcgac
    13381 agcatcgaac ttgggcgatt gcttgaccgc gtgcatgggc cgactatata agaacagaaa
    13441 acaacagcgg ggagtgtgta tgttatctgt tttgatcttc gtgcttttga tctttagcag
    13501 cgcgtcggaa gcgatttcgg ccgaaagtca gaagcttgat cgagttcgca tcggctattc
    13561 atcgatttcc agcagtcgca ttgcgctttg ggtggcgagc gacatgggct tctttaaaaa
    13621 aaatggtctc gcccccgagg ccattgttac gccgggcgtg cagggcaccc aggcgctaat
    13681 cgccggcgaa cttcagtttt atctcggcgg cgtcgattca gccgcgctgg ccgcggcgcg
    13741 aggatccgac ctggtggtct tggccaccgc agaacccatc gagtacaagc tgattactca
    13801 gccaaacgtc aaaactgtta aagaggtaag aggtaaaaag atcgttgtcg atcgggtcgg
    13861 cggcacgagc tactacatct cgctccagat cctggaaaag gtcggactga agagtggtga
    13921 ggtcgaattg gtgcaagtgg gcggcggtgg aaatcaaagg gtggcggcgt tcaagtccgg
    13981 gctggtcgcc ggtgttgtta catctccgga tcgattcgaa caattgaaaa ttccttatca
    14041 cgttttggcg gatgcgatgg agctcgggat caaagtgatg ggaaactcgt atctcacgac
    14101 ccgcagcttc cgcgatcaaa acaaggacgt ggttctgcga accgttagag cattggtaca
    14161 aggcaggcga tgggccaagg atccgaaaaa ccgccaggag gtcctgcgca tctacaatcg
    14221 ttatctgccc agcccggatt ctgcctttat ggatcatctc tatcgaaaaa atgtcgagtc
    14281 aatgccgctt tacccctata ccaatatcga agatctgcgt atttttctgt cctacttgag
    14341 cgacgccaat ccaaacctgc gcaacgtaaa attggcggag tttgtcgata acagctatct
    14401 caagcggatt gagcaggaaa gcggcagctg atcgccgcgc cgtccctaga tcgtcgtact
    14461 attatgcttg taaactttcg cagcctgcgc aaatttcttt cggaagacgc accgcagagg
    14521 cgcagagtac ggagagaaag aaatttacgt gttctctttg atcgaagttt ttgccggact
    14581 ctgcgacctc agcgcctcgg cggtaaatca tccgagttct atggttgcgg ctctgccggc
    14641 gctaggtcct acgtgcgctg ctcgaaaatt accttgaagg tgaagagacg actcccaaga
    14701 tcgtcacccc cgaatgtttt aatcgggggt ccagcggaat tccacctgga ttcccgctaa
    14761 aaagcatgcg ggaatgacag actcctccga gcattagcgc cttgaagaag acgtactcca
    14821 ttttaattct ctgtggccga gtgtaggttc atgacacctt cgtagtgaat cgctttcact
    14881 catatttttt ctttgggaga acatcacatg agcaccaccg accaatgctt gctgatagcc
    14941 aatagtaatt accacgtcgg tcaacaagtc gccgtctacg tcgcccagga acaaggctat
    15001 ttccaagagg agggcctcga gaagttcgac tacgatgggc gtggtttgat cccggcgatc
    15061 ctggagagac aaggcctcgg tccggccatg gtcgaacacg gtgtcgatat tgtaaccgca
    15121 gtggatgtct ccgcggcgat ctatcagaga tctctgggcg ctgatgtcta tgtagtcggc
    15181 ggctggcgtt acgatccgga tctgaagtgg tacagccgaa aggacataac cgatatccgg
    15241 cagctgaaag gccggcgact gggaattcgc gaaagggaag gcctggttta cggcttcatg
    15301 gcgagcttat tggtacaagc cggcatcgat ccggaaaagg aagtgacctg gatcttcgac
    15361 ccggtcttcg gttacggcaa cgatcccaga catgcggatg agttgcggca gggaaaggtc
    15421 gatgcaatgc cgtccttccc gccattttcc aaacagctcg aaagcgaagg ctttccgatt
    15481 ttgatggacc cgcggatcgt atttccgcgc cggcctggaa aagtgacggt cgcgacgaaa
    15541 agaacgatcg attcgagagc cgaagagctg cgcgcatatt tacgggcgat gatccgagcc
    15601 ttctggttca tgagagacac ggagaatttc gattatctca gagacctgga gagccgtctg
    15661 cgcagccaga gccataatga cgaagaacgg cagttgttta tcgtcaccag cgtcgaaaaa
    15721 gtcgacggct gggcgttgcc catcgacggc ggagttagcg ctgagagctt gaataggatc
    15781 atccaggaaa tggtcgccat aggaaattta aaagaaccca ttaacgtgca atcgttgctc
    15841 catgattccg cggtcagaga agcataccaa gaacttgtca agcggcccga acttcggacg
    15901 gcgcatgaca aagtcaaatt ggtcgttgag aagtatgggt tttgaactgc gtgcgcgtgg
    15961 tctgtttgat gggcgatcct tcgcatcacc cggggcaggt ttcagacctg cccctacagc
    16021 ctcggttgcc acgtcgcgag cgttgccgcc gtcgaccaat ccttgccgca aatgtaatcg
    16081 gtgaaagaag gtttcaattt tccgagcacc ccctgtagcg tgatccagat cagaatctca
    16141 ttggccactc ccgcgtgttc gagcgtttcc gccgtggctt tgtcgaccat ctcttgagcc
    16201 ttggcgtcct ttaccaagtc gaggaactcc aaatccaggg cggtatcgat ggagcccatc
    16261 ttgggtccgc cgatatctcc ggaaaagctg ccgctagcaa gcactgcgac gttgccattg
    16321 taatggttgc gcaggtaatc gccgatctgc tggccgagcc gaaaacatcg tcgggcggtg
    16381 ggccggggcg gtagcatgcc gttgatgtgt aatggcacga tcggcagatc catgcgcggc
    16441 gtcagcaggc tcagcggcac catgaaggcg tggtcgagga tcatctcttg cgaataactg
    16501 aagtcgatgc cgttttctat gccgtagtta agcagtccct tggccattgt cgtgtccact
    16561 ttcaaagtgg cgcgcggaat gcgcagctcc gccatttctt taggcgcggg tccggtgcag
    16621 gtatcggaga cgccaaggca aaacatcggc gcattgttat agaaaaagtt ggtgacgtga
    16681 tcactggtca aaatcagcag gcagtccgga gaagcttccg acaagccttt tttcagatga
    16741 ccaaaggccg agtcgagctt attctgaact tcgggggaca tcttgtcggg acgagatgtg
    16801 atattgggat tgtgtggcgc ggcaagccct agaacgatcc tacccataat ctattctcct
    16861 cgtacttgct taaagtaaat agccggtttc aaatccatca gccgggagaa ttgccagagc
    16921 agaaaactat gcgctcccat tcgataaagc tctcccgcat tgacctcccg gagcgctttg
    16981 gcctcgaaat cctccaagtc gtaacgtttg agaacggctt cgggattctg tttatattcc
    17041 tcttgcagct ccaaatcgtt gaacagggtg aacagaagtt tgtcgacttg gtacaggctc
    17101 atttgctttt cctcatctct tcgtttaccg accgcacgat cgacaaatca taaacgtctt
    17161 cgggcccgac atcgcgctcg actttgagcg attttctctg gaaggcgatc attttttcct
    17221 gaaggccgcg cgggattgtg ccatcggcgg acatcacccg caaccaagcc cgataaacgt
    17281 cagtggcttc agcttcggag accttcatga tcggcaccat gcgggcgact acgtccttct
    17341 cgttgttctt gagccagtaa aatcccttga gcgtgccgcg gagaaatctt tgaacgagtt
    17401 ccggccgctg ggccaacgtt ctttcggttg taaccgcgcc ggcggtgacg aattccaggt
    17461 cgtcgccgta aaagagcaat tcgttcaacc cgtgtttttt ggcgatgatt cgatccatct
    17521 gattgaccac cgccgcatcg acggcgccgc tcatgagcgc cgccaatcgg ttcggggtgg
    17581 gctcgatggc gcggagaacc acatcctttt gcggatcgat gccgcgcctg ggcagcagat
    17641 cgcgcgtcat ttggtccaat gccgcgccga agctcgacac agcgatttgt tttccgcctt
    17701 tcaggtcctg cacagtcttg atctctttgc gggcgaagag ccagttcagc gggcgggtat
    17761 cgaataccat cacgatcttc agcggcacgc ctctgagaat cgcgccggcg ccttggccga
    17821 gaatctgact gaaatggaag ctgccgccga ctagtccttg aattcccggc aacatccccg
    17881 cctgaatcgc tcggacgtcg agaccttctt cccggtaata gcctttctcg atggtaaact
    17941 tgtagagaag cgcattgccg ctgatccctg ccgagatgtg caggttgact tcgtccaggg
    18001 cgaacgaagc tcgcgccaaa ccaagagtac aagccagaca tgaaatcaac agcaggttcc
    18061 ttgctgggtt gcggaagatc gattttgctc ttgtggtcat gtgtgtccct ctagcctagc
    18121 agggtgctga aaaaagcctc ttgcaggctg ctcaaaaaga tccagatgcg aagcgcgcga
    18181 attacaagga tgaaagcgga aatctaaaag cggaattcag ttttcagctt tcataattca
    18241 tcttttcgaa accatatggg tctttttcag caggcctgct agcttagaat ccatatttct
    18301 cttgagccgc cagcgcaatc cgaaaagcag ttttcagctc ggtccggctg ctgacttctt
    18361 tgagagcctg cttgacggca tcgtcgcgga cgatgtctct ggcggacatg gaatgggaaa
    18421 gcttaccgga tttcaccatt tcgtcggcaa ttctttgtac cgcatcggac gcgatgccgc
    18481 cgtcgatcgg tatggcccag ctttcgaccc ggtcgggcgc ggtgacgatg gcgacgacgc
    18541 cgcgctcgtc ctcgttgtgc gtgttggttt ttctaagcct tttctcgaga tcgcggagat
    18601 actcaaaatt tttaacgtcg cgcatgaacc agaaggcgcg aatgatcgcg cgaaaataag
    18661 cgcgcagctc gtcgccgcga ttttcgatca tctgtccggt cgcgacggtg agtttgccag
    18721 ggcggcgcgg aaacacggtg ttgggatcga gaatcatcgg atatccttct ctctcgagct
    18781 gctcggaaaa cggcggctgg gaggtgatcg catcgatttt ccccgagcgg agcatttcca
    18841 tgtgagccgg attatttcga tagccgaaga cgggatcgta gacccactcc acttcttttt
    18901 ccggatcgac accggcttcc tgaagagcgt tggtgataaa aacctgaact aagccttcct
    18961 tctcgcgcat gccgatgcgg ccgccgcgga gcttgctcat atccgtgatg tgcttcgatc
    19021 cgtaccatcg gagaaaaggc gtgtaacgcc agcccccggc gatatagaca tcggcgccca
    19081 gcgaacgctg atagattgcc gcctcgacgt cgaccgcggt cgcgatatcc acgccatgat
    19141 tggtgatcgc cattgccagc ccgtcgcgct cgagcggccc gggaatcaag ccgcgggcat
    19201 cgtattcata ttccttcagt ccttcttcgc ggaaaaagcc ctgctcttca gcgacaaaga
    19261 tcgcgatctg gtgaccgacg tgatagttgc tattggcaat caaaatcggt tgatccaacg
    19321 tgctcattga gagtcccttc catgtagtgt tcgagtgctg gagtaaagga gtgctgcgcg
    19381 cagcgatatt ccgttactcc aatactctat tactccagca gtgtcatgtg ccgggcgcga
    19441 aaatttcttg gatgtcgagc cgtcgttcgg tgagtcgctg ttcaaacgcg taatccagca
    19501 aggtctccaa cactactcgg ttttgttcca ctgaataggg ccacgggtcc ggaccgagca
    19561 gcgcgcgctc ctcctcctgc tccgcgccga accaagccag agacgagcgc cgatgatcgg
    19621 cccaatatcg atacgcttgc tgcttggcgt cctcgaaagc gcggacgagc tgtgaagcca
    19681 cctcgccacc acgctggagc acttcatcct tgacgacaac caagtgacgg atcggaaaaa
    19741 tcccggtgcg gcgatagtag tccacttcga cttcgcgata gttgggccat agccgtctta
    19801 cgattttgtt gatagccgat gcatcggctc cactgtcgga cagtatttgc gcgtccaact
    19861 cgcccgcctt gagtagatcc tgcatttttc gacccgccgg cgcgtggtcg atgcgcagcc
    19921 aggaaggcgg agaccagccc ggcacatctt ccgagccatc ggcgatccag tgaatctcgc
    19981 ttgacggcag gtcgaactca tgttggagca gaccgcggat ccagagggca atgggattga
    20041 aatgcccatg aacgccgaca cggcgcccgc gcaaatcttc gggtcgaagg atgtttgaat
    20101 cgatgcgcac cataacgcaa ctgtgattga accggcgatg gggaaatacc ggaatcgcgg
    20161 ttatcggaaa tccccgactc ttaagtccga gatactgcgc aatctgcaac tcgcaaacat
    20221 cgaattcaag atttcgcacg aagcgggtgt ggcgctcggc ggaaggaagc ggaatcaccg
    20281 ttaaatccag cccgggtgta gggacactgc gatcgattag gggacgtgta cggtcgtagt
    20341 caccgcagga tagtgttagg tgagtgtcgg gcatgctacg aattgctatc ttctactttt
    20401 gtgaccgtga aagtaatctg agcataatgc attagctgca acggcttaag gatgcaacag
    20461 tgacgggccg cgcttaaccc tcaagatcac acccattcgc aatctataaa ttgtgagttt
    20521 acgcgctatt gcgacaggct gctttcggcc tgtttaacaa aagaagtatc gatgatctca
    20581 ttaggatcta ccgtctttgc ttgcggaaac tgcaccgaca tcatatcgag agcggtcatc
    20641 accgcatcag cggttggata aggcgacttc tgcatcttgg cgctgaaata gctataggtc
    20701 tcggcgagaa tatcgtcgtt tgctccccgt agatattttc gcatcacggc catgctggtt
    20761 tctttgttgg ttttaaatat ctgaatcgct tccatgaggg tcctgaccaa tctcgcggtg
    20821 atctccggat tctttttgac atttactttt aatgtcgaca caccaacata ggggtaggga
    20881 attcccattt cgtcgaagtc gagcatctcg cgaaaaccca ttcgcttcgc cagtagccgt
    20941 gtcggtgtcg tgagcacggc ggcatccacg actcctttct ccaacgccgg cagtatgctc
    21001 ggcccatcgc ccaacggaac gatcttgacg tcgcgtgcag ggtcgaggtt gagtttgttc
    21061 ttgagaatgt agtgcagcat gacgtcggta atcgcacctg gacgcccgga gcccaccact
    21121 tttccgcgca ggtctgccgg tttattgatc tgcggctttg agaacaggga agagccggtc
    21181 acgttcagaa acgttgcaat gatcaccaga tccttttggc cgacagcgta agaggatacg
    21241 acaccggttc cggtcgatat gacgtactgc gcatcgccgc taagcagcag ctggtgcggc
    21301 cgtgctcttc ccgcgtaaac cgcttccaga tccaagccgt attttcttcc caaacgttca
    21361 tccacggcga tccagagagg agtgtatccg ccggtaaacg aagaatagat gatcttcagg
    21421 cgctgcgtct cgccggcagc aaatgacgcc gaaacgaata cgaacatttg catggataaa
    21481 atcaatccaa agagctgata actgcgcttc attccgtttc tcctatgctg tgtgtagttc
    21541 tgtcgcgtga ttcgaacgac tttacaaccc agtatcttgt cgagaggccg gcgcaccgac
    21601 gggcggatca acattgcagc ttttaacgga gagacgactg cgcgctgatt tgacttttca
    21661 gccgagaagc gaccaacggc atcagagcgg gcatcaccgg tctaagcttg ccagggcctt
    21721 cttgtaaaac cgttcgtcga catatttttc gggtttgggc agtggcggtt tcatcagccc
    21781 ggcgatctct cgcagttgga gaaccgattg aatcccgtcc atgtcgattt ggctccttgg
    21841 actgaatcca aatcgagggc tgatgctttc ttcgtacatc gcttgagctc gagccttgtt
    21901 gttgcggttc tcaggcataa gcatggcaat aacttcgtcc ttgtgattcg actcagtgac
    21961 ccaatcggtg gcgcggatca tggcacggat gaaacggacc agcaggtctt cgttcttatt
    22021 ggcccaatcg cgacgagtcg cgccgagtcc tctggcatag tgctggacgt aatcttcggc
    22081 tttggcgagg actttaaagc cgcgcttttg aatttcgtca tcggcgagac tcatcatcgc
    22141 ggccgacgcc tcgccgcggc tcatcgcctc ggcgcgcttg gtggtattgc cgaaggcttt
    22201 gaacatatag tccttgctca actccaagcc gtgcttcttg agaatgtaga cgccgacaat
    22261 tgcatagccg gtggtcggcg cgtcgacggc aaagattgtg cctttgaggt cgccgtagtc
    22321 gttgatgcct gagccgacga tcagcttttg attaacgccg cggctacctc cgagaaaaat
    22381 gacgaaatca tttttttgag ggtcttcgcc ttggccttca gcccaggcga tcacgttatc
    22441 ggcattattc atgatgaggt cgtacttgcc gctgatgaga ttacgcagca gagtagggga
    22501 gtccgtgacc gtgtcgatct gtatccggat gctctctttg ttgaaaaatc ctttggcatc
    22561 ggccgcgatg atcgcgggat ccttattgaa caatccaatc tggatgacat cttcagccat
    22621 aactatgcgc ggcaggagcc atggaaagct cccgaccata tctccttgtg ttggttattt
    22681 ttagttctcc caaaacgcgc ggtaccgctg ttcgatccgt taatacagtt ctctgatcgt
    22741 tgtcaatgat agagacgcgc aagagtttac cactgagcac cgcaaagata tgcgctctgg
    22801 gaagtccgga atctaccgcc gaggcgctga gtgcagcgca gattccgccc agtgtgcttt
    22861 gtttctctcc gtgagaaaca tcagacggct caggacaaga caaagtgtct atttggatcg
    22921 gatttccttg accatagaga agtcggcgac ttcgctcaga gcgacctttg acgaaagcag
    22981 ctgggcgcgt gttgccacga catcccaaaa gctcttatcg ttgcgcggtg ctccatcact
    23041 atacgcaaat gtgacgtctt tcagcgccgc aattccttcc tgagctggca tcctcagcca
    23101 ttgttgaatg ctagctccga ccgcttgctc attactcggt tgcagaatga agcggcttcc
    23161 gcgcactaaa gcacgcagca tccgccgaac ttgattcggg ttttctttta tcgtgcgcct
    23221 atgtgtcgaa agtccagcca aaggagcgct atagatatcg ccggcacgga aaagcacgct
    23281 aaggccggcc tcgcgagctt tttgtggcca aggaggcacc aggaatgtag cgtctgcgac
    23341 accctgctcc accatcgcaa acagcgccgg ggaatcaggt gccgggatcc atttgacatc
    23401 tttaagggga tccaaaccaa agtgacgaag caccgcttga aaaaccaagc cggtggaccc
    23461 gctcagatcc acggtattga gcacaaaggt tttaccgcgg aggtccgcgt ccgacttgac
    23521 ggtgggccgg gttaccaaat aatggagcga acggtcgacg aagaccgcca acatctgcat
    23581 cggtacccct ctgagagcgc tgtctatggg aggaagaaag ctcattgtat agtcgatatg
    23641 gccgtttgcc agcgccggta tgagggcggc cgtgcgcatt tgaatgaatt ttacgtcgag
    23701 tccttcttga cggaaaaagc cctgatctac cgctaccctg ccggggagaa acgtcaacgt
    23761 ggaagcggtg taactcagag ttacctcctc ggccgtagag agagccgggt ttgagactga
    23821 gtagaacaca gaagccaaca gtaaaaagcc gcaggtgata tgtttcaaat ggttccttcc
    23881 tttgatttcc cgaccggagt aaacacagat tatcaggatc tgtgatcttc tgtattccga
    23941 a
//
LOCUS       DASUVS010000437         3533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_192632, whole genome shotgun sequence.
ACCESSION   DASUVS010000437 DASUVS010000000
VERSION     DASUVS010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192632"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..985)
                     /locus_tag="VF182_23075"
     CDS             complement(137..985)
                     /locus_tag="VF182_23075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6440020.1"
                     /translation="MSENPHAFWRRLAEAALQLCQAGTAGVSLLDLKDGEDVFRAEAV
                     AGVLSNRLESTMARASPCGAAIDRDATQLMHLPERFFSNMRFDPPIIEALIIPFRVQN
                     RPAGAVWFLAHDEVCKFNREDERIGTTLSSFAAAGWHLRGARADAESTIENHRSSRLQ
                     ESAASTEQIIEPQHMREELQQLTQSLEARASEKTAELIAGEGVEMPRDPAEEPQQSWL
                     DENATAQSEMGDFNRLLTIIQGYTTMMKADLGDPTKLKEDIEAMSEAASLVQTLINSH
                     WAQRLG"
     gene            complement(1681..3363)
                     /locus_tag="VF182_23080"
     CDS             complement(1681..3363)
                     /locus_tag="VF182_23080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEX6440021.1"
                     /translation="MSNGPQRPPIDDLWYKNAIIYCLDVEKYVDSNGDGIGDFGGLTR
                     RLDYLAGLGITCVWLQPFYPSPNRDNGYDVADYYGVHPKHGTLGEFVEFMNHAQQIGL
                     RVIVDLVINHTSDRHPWFQAARKDPRSDYRNWYVWSKTRPAEWNKGMVFPGVQKATWT
                     RDPVADEYYFHRFYEFQPDLNTLNPAVEREMMRIMGFWLQLGVSGFRMDAVPFMIEQK
                     GAGVTPRKDYEMLHLLRNFLQWRRRDAIFLAEANVPPNESMEYFGENGERIQMMLNFA
                     VNQRVFYALATGDIRPLVRALEATYNRPPAAQWVNFLRSHDELDLGRLTDTQRERVFQ
                     AFAPAKEMQLYNRGIRRRLAPMLDNDRRKLELAFSLVFTLPGTPMLQFGDEIGLGDDL
                     SLPERECARTPMQWSTDPHGGFTTGKRPVLPVISDPIYGYQRVNVEGQRRDPHSMLNW
                     MERKIRMRRECPEISWGDWKILKTDQPGVLIMRYEWDDHTLATLHNFTAKPCAVVLDR
                     AAINADSGAGQLVDLLAHNDSRADDNGRYVIELQPYDYRWLRAGGIDRNVAR"
BASE COUNT          772 a         1014 c          951 g          796 t
ORIGIN      
        1 gttgtagcgc tgtatcgatt ggggagcctg gcgcgcgagt gcggctctct ttcagagaag
       61 aatcctatag ccgacaaacc gacatgaatc cgtgccatca atgctaccac gaaggctgtt
      121 cgcgctcatt gaacggctag cccaaccgtt gcgcccagtg agaattgatc aacgtttgca
      181 ccaacgatgc cgcttcgctc attgcttcta tatcctcttt gagtttcgta gggtcgccta
      241 agtcggcttt catcatcgtg gtatatccct gaataattgt taggagccgg ttgaaatcac
      301 ccatttcgct ttgagcggtc gcattttcat ccagccatga ctgctgcggc tcttccgccg
      361 gatcccgcgg catttcgacc ccctcaccag cgatcaactc cgctgtcttc tccgaggcgc
      421 gggcttcaag gctttgggtc aactgctgaa gctcttcgcg catatgctgt ggttcgatga
      481 tctgctctgt acttgccgcg ctttcctgga gtctactgct tcgatgattc tcgatcgtgg
      541 actcggcgtc ggctctcgcc ccgcgtaagt gccaaccggc cgcagcgaag gacgacagag
      601 tcgttccaat ccgctcgtct tcgcgattaa atttacagac ctcatcgtga gcgaggaacc
      661 aaacagcgcc tgccggccta ttttgaacac gaaaaggaat aatcagcgct tcaatgattg
      721 gcggatcaaa tctcatattt gaaaaaaatc gctcaggtag atgcatgagc tgcgtcgcgt
      781 cacgatctat ggcggcgccg catgggctgg cccgggccat tgtgctttca aggcgattgc
      841 tgagaacacc cgcgacggct tctgctctga atacgtcttc tccatctttc agatctaaca
      901 ggctcacccc ggcggtgccg gcttgacaga gctgtaaagc agcttccgca agccggcgcc
      961 aaaaggcatg cggattctcg gacatagcat taaccaatat tttaaaagtc tgtatttcag
     1021 ctctgtaatc cggtgatcgc gagggccgga gggaaagctg cgacgtcacg atgacatcat
     1081 ccaaagacac tggtgattcg cccattgtgt tcctcacggc cggtcgcatc gagcagggtt
     1141 ctaatgcgcc gcaagaagtc gctgaggtga ccggggatac ctgcggggtt cgaaaaaaca
     1201 actgaaacga tctatcaatt cacagcacgc tctgtatatc gtgcgcgagc atttcaagtc
     1261 aacaataatc ggcgatacta gcgcttgtgg aacagagtcg ggaaagcagc gccgggtgat
     1321 cgtcgctgct ttaccgcggc ttcaatgacc tcgaccgccc ctgggcagct cttttttgcg
     1381 ttctcgagca aaatacgggc atctcgttca tcacaaacca attcgtaagt gtcatcgaac
     1441 atagggcgag ccgagcggac ctgagtggcg cgatccagta gggtacgaac tctcgacgcg
     1501 gccgataggc aggacagcag ggactctcga tgttcgggga tcaatgtgat ccgaactttc
     1561 gccatacaag aaagttaagt tgcgatggta cggatggcaa gggagaaata aaccgtcgca
     1621 tacgttttta ttgtcctaaa tcggctgccg gagccgttcc agattccaag agctgcgcct
     1681 tcatctcgcc acattgcgat cgatccctcc ggcgcggagc catcgatagt cgtagggctg
     1741 cagctcgatc acataccggc cattgtcgtc ggcacgactg tcattgtgcg caagcagatc
     1801 gacgagttgg ccagcgccgc tatcggcatt gatggcagcg cggtcgagca cgacggcgca
     1861 gggcttagcc gtgaagttgt gcagggtcgc cagcgtgtga tcgtcccatt cgtaacgcat
     1921 gatgagcaca ccgggttggt cggtcttcaa aattttccaa tcgccccagc tgatctccgg
     1981 gcactcccgg cgcatccgaa tcttgcgctc catccagtta agcattgaat gcggatcgcg
     2041 acgttgccct tcgacgttga cccgctgata gccatagatc ggatccgaga tcaccggcaa
     2101 aaccggccgc ttcccagtcg taaatccgcc atgcgggtcg gtggaccact gcatcggcgt
     2161 ccgcgcgcac tcacgttcgg gaagtgacaa atcatcgcca agcccaatct cgtcaccgaa
     2221 ctgcagcatc ggggtgcccg gcagtgtaaa caccaaacta aaggcgagct ccagcttccg
     2281 gcgatcgttg tcgagcatcg gcgcaaggcg gcgccggatg ccgcgattgt aaagctgcat
     2341 ctctttcgct ggcgcgaaag cctggaacac tcgctcgcgt tgcgtgtcgg ttaaccggcc
     2401 aagatcgagc tcatcatggg agcgcaggaa gttaacccat tgcgccgccg gcggccggtt
     2461 gtaagtagct tccagcgctc gcacaagcgg cctgatgtcc ccggtcgcca gggcgtaaaa
     2521 cacacgctga ttgacggcga agttcagcat catctgaatg cgctcgccgt tctcaccgaa
     2581 atactccatg ctctcgttag gcggcacgtt ggcctccgcc aggaaaatcg catcgcgccg
     2641 gcgccactgc agaaaattgc gcaacaaatg aagcatctcg taatccttcc gcggtgtaac
     2701 acctgcgcct ttctgctcga tcatgaaggg aacggcgtcc atccgaaaac cggagacgcc
     2761 aagctgcagc cagaatccca tgatgcgcat catttcgcgt tctacggcag gatttaacgt
     2821 gttcagatcc ggttgaaatt cgtagaaccg gtggaagtag tactcatctg cgaccggatc
     2881 acgggtccaa gtcgctttct gcacccccgg aaataccatt cccttgttcc actcggctgg
     2941 gcgcgtcttc gaccacacat accaattgcg gtaatccgac cttggatcct tgcgcgccgc
     3001 ttgaaaccag ggatggcggt cggacgtgtg gttgatcacc agatcaacga tcacacgtaa
     3061 accgatctgt tgcgcatgat tcataaactc gacgaactcg ccaagcgtgc cgtgctttgg
     3121 gtgcacgccg taataatcgg caacgtcgta gccattgtcg cggttcggag acgggtaaaa
     3181 cggctggagc cacacgcagg tgatgcccaa cccggcgaga tagtccagac gccgtgtcag
     3241 gccgccgaag tctccaatgc cgtcgccgtt tgagtcgacg tacttttcga cgtcgagaca
     3301 gtagatgatc gcgttcttgt accagaggtc atctatcgga ggacgttgcg ggccgttgct
     3361 catcgtgatg ttttcctttc ttcgtgtgat ccgccgctgc gatcggacgc aggagtctta
     3421 gccgtcagcc aggaatggct aaatgtatca ttatttcgtg aacactacct ctaaacgcct
     3481 ctcctactcg cttagcgtag agaggtttca atctagctca taaataactt tat
//
LOCUS       DASUVS010000438         6225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_414040, whole genome shotgun sequence.
ACCESSION   DASUVS010000438 DASUVS010000000
VERSION     DASUVS010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414040"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..370)
                     /locus_tag="VF182_23085"
     CDS             complement(179..370)
                     /locus_tag="VF182_23085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440022.1"
                     /translation="MHRTSARIPHLKLSEGHDGYIGYKGRRMLSILNKQKLERVLAYL
                     LDENEFLGPHGIRSLNDDD"
     gene            complement(502..900)
                     /locus_tag="VF182_23090"
     CDS             complement(502..900)
                     /locus_tag="VF182_23090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440023.1"
                     /translation="MNRSLSFKIILFLAFLQGVFGLVRAYDWVQIGSDLFGEGALLLP
                     FVGAMAVMRGLIISFVALLYMLSVFGALLAKPWAWWTGLIAVVLNLLLVLPGLAHGAS
                     VTEVLAWSAIPLILLFYLFSPRRSDALGRA"
     gene            complement(978..2276)
                     /locus_tag="VF182_23095"
     CDS             complement(978..2276)
                     /locus_tag="VF182_23095"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HEX6440024.1"
                     /translation="MNFLLAKEQLELSFGGALAQQTIDKIFASFPTKDSALGGEIQLS
                     ASLTNPVRVSAEGHLDGSNLWLPLGSEKVLLERFKIEASGESLRVRSADLRWSKSRLA
                     VSGKIAGARENLRLDLDVSGEQLDWQDLQRIFAGGGKQRQPENFGGVSFPAVEGMIRL
                     KTNRFAFERLNLTGVDTTANISASGIRAKIDQAVVCGINVTGLVDVVGSEIRIDVRLG
                     AKGAQLEPTTVCLTNRQNEVTGTYSLQAHVLARGERDQLSSALDGIFELSASDGEFIR
                     SPGIDATFDYLNTTGDFQVAFPDLDRQTFPYRFVGIKGRIEGKMLIGDEINVSSSLLN
                     LSGQGKVDLGRNQIDGKGLIAVLKPVDEVIKRIPGINLMLGGSLVGIPVRVTGELEHP
                     NVTYLSPADIGAELLNIPLRILGMPLGAMRLFTPGGNPGD"
     gene            complement(2337..4586)
                     /locus_tag="VF182_23100"
     CDS             complement(2337..4586)
                     /locus_tag="VF182_23100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440025.1"
                     /translation="MKLPRALIFVGSAAAIALAVGVASMLLAPWLIDSRLVKDKISSE
                     LAKIYGNVTFDKIALVWFPRPTIAIENAEISFADQTRASISSIKIYPSIYHLLTGRLI
                     VRQALLQEPRLRIRLPRSSQTSFDVEKWEEQIRSALVRLTKEWPAPHIELSQGSAEIG
                     IDDKPPILLQDVAARTGASPEEVQFTITARSNLWEQLRVEGSISPVNLESQLKVGMQQ
                     FKIRETLAVVAPQFAEYSTHGEASFDVKIASVGLRNVNSSIDGSVGPFVFAGPDGSAT
                     IEATTLRGEITYDGTVLQVNVDQLELASPRLQASGNFQIQSGSLSARVNVRDVEIAEL
                     SKLALIVDGSQAVKNVLRYIPAGTVAEMNFQSAGPSLTEMASSQNIALVASLRDCTIV
                     IPGYDLEFKNAGGSLRIADGVLEASGIAANLGRAKGWNGNLRLGLDGKSAPFHLDILA
                     QSAAPDLQAVLLKLVHDEALRGELLQLRNISGELSGRLVLGESLDAISPVVTISKLAI
                     SAIYAHMPFPIEIRGGRFSYDQRFMKLESAQGSVGRSIFDGLSVSLNHNGSRRVDVGS
                     ARISLDLQETEILLERFKKVGAHLAKLRSARGSIDFERLTLSGPYDDPAAWIFAFAGK
                     FQRVEITHADLPAPVTLGREQFTANQEKVDFSEIAMAIADASWLGSGSFEYVKQEPPF
                     VVIKGTGTIGAQMTQWLSHQIEWPEELKLRAPWKVAAERLAWREDRNISFNGQVTAAG
                     GPQLSVVAV"
     gene            complement(4974..5531)
                     /locus_tag="VF182_23105"
     CDS             complement(4974..5531)
                     /locus_tag="VF182_23105"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX6440026.1"
                     /translation="MKKVFLSLVSWALISSLAGVAIVAAAPRQGASELQVSGGFFHAE
                     DTDFGNLNLDLSYGYYLSPGWQLGFRQALNYLFVDDASDQWIATTAPFINYHFRVTDI
                     IVPYIGAFIGAAWNDRDITGTLGPQLGVKLFVHEQTFVNIGYRYEWFWDKFERINNES
                     DNGNHIFNIGVGFTWGGSGAGTRRP"
     gene            complement(5701..6225)
                     /locus_tag="VF182_23110"
                     /pseudo
     CDS             complement(5701..6225)
                     /locus_tag="VF182_23110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876728.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
BASE COUNT         1511 a         1713 c         1589 g         1412 t
ORIGIN      
        1 cggtaataaa tagtatcagc tcccaattta atgggtggtt accgccggtt cattaacgtc
       61 aggcgctacg tcctgctatt caaggcagta tcgacaccgc tggtcgtatt atctcgattc
      121 atactaagcc ttctccatat gttcctttcc cagccagtat gaacgatgtt tctataagtt
      181 aatcgtcatc gttcagagaa cgaatgccat gaggtccgag aaactcattc tcgtcgagca
      241 ggtaggcgag cacgcgctcg agcttctgct tgttgagaat cgacaacatg cgccgccctt
      301 tatagccgat gtagccgtca tgcccctcgc tcagttttaa gtgcggaatt cgggctgaag
      361 ttcggtgcat gaagatgtcc ttgtcgcgca tggaggttat ctcgaaagaa tcggcaggag
      421 aagtgtaatg tcgctgacca gcaaacggcg ggctcccgat gggctaatca aaatcagagc
      481 atgtcgctgc cgggaatgac gttacgccct cccgagtgcg tcgctccggc gcggagaaaa
      541 cagataaaac aacagtatca acggaatcgc cgaccaagcc aacacctccg tcacagaggc
      601 tccgtgggct agaccaggaa gaacgagcag caggttcagc accacggcga taagaccggt
      661 ccaccaggcc caaggcttcg ccagcaacgc gccgaaaacc gacagcatat acaagagcgc
      721 aacaaacgaa atgatcaggc cgcgcatcac tgccatggca ccgacgaacg gcaatagtaa
      781 cgctccctcg ccaaacaggt cgctgccgat ttgcacccaa tcgtaagcgc gcaccaagcc
      841 aaaaacgcct tgcaaaaacg ctaaaaacag gatgattttg aagctcaaag atctattcat
      901 aaagacctct ggtattgtgg attatctctg cgtggagctt tcacgatttt ttggctgaat
      961 ttatcggagt catgttctta atcacctgga ttgccgcctg gagtaaacag cctcatcgct
     1021 ccgaggggca taccgagtat tctcaatggg atatttaaca gctccgcgcc gatatcggcc
     1081 ggtgacaaat aggtaacatt ggggtgttcg agctctcccg taactcgcac gggaattcct
     1141 actagagaac caccaagcat caagttaatt cccgggatcc gcttgatcac ctcgtctacg
     1201 ggtttgagga cggcgatgag gcctttgccg tcgatctggt ttcgacccag gtcgaccttg
     1261 ccctggccgc tcaggttgag cagggatgag ctaacattga tctcatcgcc tatcagcatc
     1321 ttgccttcta tcctaccttt gatcccgaca aagcggtagg gaaacgtctg cctgtccaga
     1381 tccggaaatg cgacttgaaa gtcaccggtc gtgttgaggt aatcgaatgt agcatcaatt
     1441 cccggcgaac gaatgaactc accatcggag gcgctgagct caaaaatacc gtcgagcgcg
     1501 cttgacagct gatcgcgctc gccgcgcgcc aggacgtgcg cctgcaaaga ataggttccc
     1561 gttacctcgt tttgccggtt cgtgagacag actgtggtcg gctcgagttg agcgcctttg
     1621 gccccgagcc ggacatcgat tcggatctcg ctgccgacga cgtcaacgag tccggttaca
     1681 ttgatcccgc agacaaccgc ttgatcgatc ttggctctga ttccggacgc tgagatgttt
     1741 gccgttgtat ccactccggt caagttgagc cgttcgaatg cgaagcggtt cgtcttaaga
     1801 cgaatcattc cttccaccgc aggaaaagac acgcctccga agttttctgg ctgccgctgc
     1861 ttgccgccac cggcgaaaat acgctggagg tcttgccaat caagctgctc cccactaacg
     1921 tcgagatcca gccgcaggtt ctctctagct ccagcgattt tgccggaaac ggccaaacgg
     1981 ctcttactcc agcggagatc tgcggatcgg acccgcaggc tctccccgct cgcctcgatc
     2041 ttaaatcgtt ccagcagcac tttttcactt cccaaaggca gccataggtt gcttccgtct
     2101 aaatggcctt cggctgagac cctcacggga tttgtcagcg aagcgcttag ctggatctca
     2161 ccaccgagcg cgctatcttt ggtgggaaaa gatgcaaata ttttatcgat cgtttgctgc
     2221 gccaaggcac cgccgaaaga caactcgagc tgttccttag caagcagaaa attcacacgg
     2281 gcgtgctgat cgccatcctc aatagtaaga ttctgcaggg cgagccactg aggattttac
     2341 accgccacga ccgaaagctg cggaccaccc gcggcggtca cctgcccatt aaatgagatg
     2401 ttccggtcct cgcgccacgc cagacgttct gccgcgactt tccacggggc acgcagcttc
     2461 aactcttcgg gccattcgat ttgatggctg agccactgag tcatttgcgc gccaattgtt
     2521 ccggtcccct tgatcaccac aaagggaggt tcttgcttca cgtattcgaa tgaaccgctg
     2581 ccaagccagg aagcatcggc gatcgccatt gcaatctcgg agaaatcgac cttctcctgg
     2641 ttggcagtga attgttcgcg gccgagagta accggtgcgg ggagatctgc atgggttatc
     2701 tcaacccgtt gaaacttgcc cgcgaaggcg aagatccatg ccgcaggatc gtcataaggc
     2761 cctgaaaggg tgagtctctc gaaatcgatt gagccgcgcg cagatcgaag ctttgctaaa
     2821 tgtgctccca ccttcttgaa ccgctcaagc aaaatctctg tctcttgtaa gtccaaggaa
     2881 attcgcgccg agccgacgtc tacccggcgg cttccattat gatttaaact cacgctcagc
     2941 ccgtcgaaga tagaacgtcc caccgaccct tgggcgcttt caagcttcat gaatctttgg
     3001 tcatagctga accgcccgcc gcgaatttca ataggaaacg gcatgtgcgc gtatatggcg
     3061 cttatggcca atttcgaaat tgttacaacc ggcgaaatcg catccagtga ttcgcctaaa
     3121 accagtctcc cggacagctc gcctgaaata tttcgcagct gcagcagttc tccacgcaag
     3181 gcttcgtcgt gtacgagctt tagcaagacg gcctgcaggt ccggcgcggc gctctgcgcc
     3241 aagatgtcga ggtgaaaagg agcgctcttt ccatcaagcc caagccttag atttccgttc
     3301 cagcctttcg cccttccaag attcgccgcg atcccgctag cttccaaaac gccgtcggca
     3361 attcggagtg agccgccagc attcttgaac tccaagtcat acccagggat cacgatcgta
     3421 cagtcacgca gcgatgcgac cagggcaata ttctggctcg aggccatttc agtaagcgat
     3481 gggccagcac tttgaaaatt catttcagcc actgttccag ccgggatgta acggaggaca
     3541 ttcttgacgg cttggctccc gtccacgatc agcgccagct tgctcaactc ggcaatctcg
     3601 acatcgcgga cgttaacgcg agcggataaa gaaccggatt ggatctgaaa gttccctgaa
     3661 gcttgcagcc gtggcgacgc gagttctagc tgatccacgt tgacttggag aaccgtgccg
     3721 tcgtaagtga tctctcctct taatgtcgtt gcctcgatag tagcgctacc gtcgggtccg
     3781 gcaaaaacga aagggccgac ggatccgtca atcgacgagt tcacgtttcg tagaccgacg
     3841 gaggctattt tgacatcgaa gctcgcctcg ccatgtgttg agtattcggc aaactgcggc
     3901 gcaacaacag cgagagtctc tcttatcttg aactgctgca tgccgacttt taactgtgac
     3961 tccaggttta ctggagaaat gctcccttca acccgcaatt gctcccagag attggagcgc
     4021 gcggtgattg taaattgaac ctcttcaggc gaagcgcccg ttcgagccgc cacatcttgg
     4081 agaagtatcg gcggtttgtc atcgatgccg atttcagcgg atccttgaga gagctcgata
     4141 tgcggcgcgg gccactcttt cgtgagacga accaacgcag agcgaatttg ttcttcccat
     4201 ttttcgacgt cgaatgatgt ttgcgagctt cgcggcaaac gaattctcaa cctgggctcc
     4261 tgcaacagag cctggcgcac gatgagccgg ccggtgagca gatgataaat cgacggatag
     4321 atttttatgc tgctgataga agcgcgggtt tgatccgcaa aagaaatctc ggcgttctcg
     4381 atggcgatgg tgggccgggg aaaccacaca agggcgattt tatcgaaggt gacgttaccg
     4441 tatatttttg ccaactccga gctgattttg tccttaacca gccggctatc aataagccat
     4501 ggcgcaagca acatgctggc gacgcccacg gccagtgcga tcgcagcagc gctgccgaca
     4561 aatataagcg cgcgcggtag tttcatgcga ttagcggcgt cggcagcaat ctactgctag
     4621 attctgttct ctcgccacgg cgagatgaat aagccgcgct agacacttct cgcgcagctt
     4681 gccctgagcc ggagagctcg acgtcacgat tgatcagtgg gtcattccga accgacacgc
     4741 cctatgaaag tcggttgctg tttccttcgg gcatgattcg ataccggcta gctcaccaca
     4801 gtattacgtg ttccaagcaa cataagatga tgcatgatgc atgatgcacc gaaccatcat
     4861 gcgttttcag cgagacaagt taacccgcag cttctcaccc tcagtttccg atgaaggagg
     4921 cgtgaggatg agaagccaaa gactgcgata aatgcgtgga aacgaaggtc aggttacggt
     4981 cttctcgtgc cggcgccgga accgccccat gtaaagccaa caccgatatt gaatatgtgg
     5041 ttgccattat cggattcatt attgatgcgc tcgaacttat cccaaaacca ttcgtagcga
     5101 taaccgatat tcacaaaagt ttgttcatga acgaagagtt tgacacctaa ctgagggccc
     5161 agggttcccg tgatatcgcg atcgttccac gcggcaccga tgaatgcgcc gatataaggg
     5221 acaatgatat cggtcactcg aaaatggtag ttgataaaag gtgcggtcgt ggcgatccat
     5281 tgatcgctgg catcgtctac gaaaagataa ttgagcgcct gtcgaaagcc taattgccat
     5341 cccggcgaaa ggtaatagcc ataactcaaa tcgaggttta aattgccaaa gtcagtatct
     5401 tctgcatgga aaaatccgcc cgatacttga agttcacttg caccctgtcg gggtgcggca
     5461 gcgacaatcg caactccagc gagcgaactg atcaaagccc aactcactag agacaggaaa
     5521 accttcttca tagaacacct ccactgttaa attcgaagct tttgatgaac cgtgaaaatg
     5581 ccacactaca tgatcgcggc cgtcgcccgg gcaacatggt tgccagtggg cggaaggacg
     5641 caactaaata aaacggactg taagcgttca cgtgcatacg gcttgttcgg acagtttcca
     5701 ttacaggata aaatccgttg aaagaaatct ggagcttctc tccgtgacaa tgcattttag
     5761 gatccgtcta tttgcatcgg tctccttggc cgcgacaagc gtccgaatcg tgaagaccct
     5821 cagggcatca gaaacggaca gggtaccttc tgcggaatcc tttctgcctg tgaatggaaa
     5881 cgtatcaggg cctctttggc actggctgtt gatattgacc cggcagacct gattcactag
     5941 agggtcgatc agatcagcaa ttatctgcgg atcgcttcca aaaaggctaa cctgctgtcc
     6001 ataatccgac tgaatgacgt agttcatcgc ctcatcaaac tccgagaagg aagcgatcgg
     6061 aacaacaggc ccgaactgct cttcagaata caacctcatg gtaggatcaa cgggatacac
     6121 gatgcaggag acatgaaagt atgttcatcg cagagatagt gactttgctc catcggtgag
     6181 gcgatgcgcc acttctccgg tattgcattc tcggcaggaa aaatc
//
LOCUS       DASUVS010000439        13154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1943689, whole genome shotgun sequence.
ACCESSION   DASUVS010000439 DASUVS010000000
VERSION     DASUVS010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13154
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1943689"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VF182_23115"
     CDS             complement(<1..659)
                     /locus_tag="VF182_23115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(162..164),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6440027.1"
                     /translation="MGALTQSGCGPAVPPLKTRGATNTTTVCPFCGVGCGQVVSSRAG
                     KVIHIEGDPGHPISEGTLCSKGAAGIQVVNNSRRLQKVLYRKPNGAAWEEKSWDWALE
                     RIAARIKETRDKTFKTTIDGRVVNRTEAIACLGGSALDNEEAYLLVKLMRALGLVYVE
                     HQARLUHSSTVAGLAASFGRGAMTNHWVDMLNADVVLIMGGNPAEDHPIAMNWLARAK
                     E"
     gene            complement(1467..2888)
                     /locus_tag="VF182_23120"
     CDS             complement(1467..2888)
                     /locus_tag="VF182_23120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HEX6440028.1"
                     /translation="MAEARDLDASTRRRRRLFFIIVGAALLATIVGVGLLMNIFERKQ
                     EARNPFYRVVELTDETEDPAVWGKNFPLQYDDYKRTVDQVRTRYGGSEALPHTPTKAD
                     PRSVVAQSKIEEDPRLKTMWAGYAFAKDFREERGHAYMLDDQTLTERQKVVQQPGTCI
                     QCHGSVYVPYKKAGDGDLIKGFEKFNQMPFFEARKLVSHPVACIDCHDPGNMQLRVTR
                     PGFIEGIAAFKASQGIQNYDVNKQATRQEMRAYVCGQCHVEYYFKGPEKRLVYPWAKG
                     LKVENIQAFYDEAQFKDWTHADTGAPVLKAQHPEFEMWNQGIHARSGVTCADCHMPYK
                     REGALKISDHHVRSPVLNINRACQTCHKWPEEELKQRVETIQERTFKMRNVAMDALMD
                     LIKDLKAAKSAGKTDKELAAAQDFQRKAQFYLDFVEAENSTGFHAPQEAARILGESVD
                     FSRKGQIVLRDGKSEARAQTGKP"
     gene            complement(2928..3386)
                     /gene="nrfH"
                     /locus_tag="VF182_23125"
     CDS             complement(2928..3386)
                     /gene="nrfH"
                     /locus_tag="VF182_23125"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020477575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HEX6440029.1"
                     /translation="MKLAPIYAIVLGIIAGVAIGIGAYTFAYAKGWSYLTDDPAACAN
                     CHVMQEQFDGWIKSSHRSVATCNSCHTPHNFIGKYATKASNGFWHSFFFTTGGYEDNI
                     QIKPHSREITERACRGCHGEIVDAIEPSHLSSAGGQLACLSCHNSVGHLH"
     gene            complement(3569..4258)
                     /locus_tag="VF182_23130"
     CDS             complement(3569..4258)
                     /locus_tag="VF182_23130"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX6440030.1"
                     /translation="MSEVSAQRASWLRDFWRAYFHRRYTILFYTLFLTLVAAPLLSAF
                     EFKGVLIELFLAANLLAALIPVATVRSRRILFVATVLVWLARPATAWLDHSALSVMTL
                     GLWTVLALFGAAGALRFALDNEPIDAEHLYAALSAYMLAGIFFGLFYWVVEQTEPGSF
                     TAAADFTRMSGFYFSFVTLATLGYGDIVPRSDVVRGLAIVEGVGGQLFLAVMVARLVS
                     LYVKAEPKTKS"
     gene            complement(4255..5412)
                     /gene="cax"
                     /locus_tag="VF182_23135"
     CDS             complement(4255..5412)
                     /gene="cax"
                     /locus_tag="VF182_23135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEX6440031.1"
                     /translation="MEFHYSNTLKSFFPTEQRIYHPQEFTVRMNWLFIFVPVAVALEH
                     ASPQAHTWIFFAACVAIIPVASLIVNATEHIAVRTGDAVGGLLNATFGNAPELIIAVV
                     ALKAGLFEMVRASLIGAILANLLLALGVAFFLGGLRYHDQTYNPAAARVYSSMMLIAV
                     ISLVVPSAFSRLFSSDVIVRDEQLLNLGMVVVLLIAYALYLVFMLKTHPEFFSSSDTK
                     AEDHHVGQQWGLPRAIASLLAASALAAWMSEILVGAAEGTGKALGMSQMFIGIIFLAI
                     VGGAAESGSAISMGRKNKMDLAVSIALGSCIQIALFVAPALVLLSYAVAPQPLELSFG
                     RAELGSMFLAVLIGAIVSGDGRANWYKGVQLIIVYTIMAFLFYFMPEVMPG"
     gene            complement(5530..6927)
                     /locus_tag="VF182_23140"
     CDS             complement(5530..6927)
                     /locus_tag="VF182_23140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX6440032.1"
                     /translation="MRAVALAAILAFFIGGCTLGPDYTRPGVPIPDNHRGAVEPPKAE
                     SLADIPWWELFNDNVLQDLTREALRNNYDLRTAAARVEEARAQIGVTRSFLYPRFDAN
                     VGGSAQQVSGLTDPPESFGSRRSFQNWFGVFNMVWEIDVFGRIRRQTEAATAIYVATE
                     MDRRGVLIALIADVAQAYFNLRELDLELEVTRRTLQLNDETTQFYRKRLDGGVSNRLE
                     LNQALANRARTAAVVPDLERRIVTQENLINFLLGRNPGTIPRGPVLTAQYYPPTVPAG
                     LPAALLERRPDVQSAEQLLVAANANIGAARALYFPNFSLSAALGGTSHDLSDLADKRA
                     AIWSVSGGILQPIFQGWRIFWNYESTIARFDQLLAQYEKAAQNGFREVADALIAIEKF
                     KDVRAEQEAQVAALQDASSLSRLRYDTGLANYLEVLIADQDLFDAELELARTRGAELN
                     SVVQLYRALGGGWQP"
     gene            complement(6928..10083)
                     /locus_tag="VF182_23145"
     CDS             complement(6928..10083)
                     /locus_tag="VF182_23145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEX6440033.1"
                     /translation="MANFFIRRPIVAIVISIIMVLMGLFTLKGLSIEQYPFLAPPTIR
                     VTATYPGASAVAVEQSVATPIEQEVNGVDKMIYMKSSNTSDGRMQLDVNFEVGVDQDT
                     ANVLTQNRVSSAQARLPQEVVQQGVTVKKQSPSILMLISLYSPKESYDANFLINYSGI
                     NLRDQILRIPGIAQVDLFGGADYGMRIWLRPDRLARLGLTPADVISAIKEQNLQAPAG
                     RVGMAPSPKDQEFTWTVSAPGRLVNPEEFENIIIRESSTGAQIRVKDVGRAELGSQDY
                     NSFGRLDGKAAGTMAVYLLPGANQLQASEAIYQTMSNAKALFPPDMDYKIVYDTTPAV
                     KASIDGIVHTFIEAVVLVTLVVFIFLQNLRATFIPLLTVPVSLIGTFIFFPLLGFSVN
                     TLSMFGLVLAIGIVVDDAIVVVEAVMHHLEHGMNPKEATIQAMKEVSAPVIGIALILS
                     AVFIPVAFLGGLTGRMYQQFALTIAISVLLSAFNALSLSPALCAMFLRAPKPARGPLG
                     KFFSGFNWVFDRSTTGYVNTTRLLVRRSILTILIIAAVAVGAGLFGGALPAGFIPEED
                     QGIFGVNVTLPPAASLERTGKVLAQVEQILTKIEGVDSFTTIGGYGVVTSTYQPNFGT
                     IFVRLKPWDDRHSDALHVRGIMAGLQRQLANIPEAIIFPFNIPTISGFGASAGFNFLL
                     QDRSGSLSVQQLGDLSRQFLEAARKRPELANLFTSFDPRYPQIKVDLDREKARKLGVP
                     VNEVFQALSAVMGGNYVNDFNRFGRLFRVYVQADADYRRKPQDIGDIYVRSETTKGMI
                     PLSTLVTITPESGTEVTNRFNLFRSVEINGVPGRGFTSGQALATLEDVFKQTMPKEMG
                     FAYSSLSYQEKVAPPAGPTFILAIVFVFLLLAAMYESWRLPWAVLLGSPLVALGSFFG
                     VWLMGYDNNVYVQIGNIMLIGLAAKNAILIVEFAKAKHEEGMSLEEAALESARLRFRP
                     ILMTAFAFILGVVPLMIASGAGAGAQNVMGTAVFWGMLVATFLGVFLIPGNFAFVEGL
                     GRKKKLAPAAIPVVPSHEGAH"
     gene            complement(10089..11303)
                     /locus_tag="VF182_23150"
     CDS             complement(10089..11303)
                     /locus_tag="VF182_23150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX6440034.1"
                     /translation="MNRNIHGVTRTGSTAILAVLMTCLVLAAACSKKEAPPPPAPPEV
                     QVAEVVQKDVPIYIELVGATLGSEDVEIRARVEGYLVSMNFTEGTFVKKGQLLYKIDP
                     QPFEAALNEAKANLATSRARVDKTNNDVARLTPLAKQQAVSQQELDNALSAQEASQAQ
                     VAAYDAKVAKAKLDLAYTTITSPIDGLIGTTQKKVGSLISGGETLLNVVSQINPILFR
                     CAIAEAEYLRLARRGAGRDKTQNQKFGVELILADGSVHPHKGRLDSIERAVDPTTGTL
                     SGQFSFPNPERILRPSQYGRARIVTEVKQEAVLVPQRAVQEIQGLYSVMVVKPDETVE
                     QRMLKAGERIGNLWIIDSGVKPGEKVIVEGIQKVRPGVKVSAKLEKSEAADAFGQKPP
                     PMDGNPAETKGK"
     gene            complement(11520..>13154)
                     /locus_tag="VF182_23155"
     CDS             complement(11520..>13154)
                     /locus_tag="VF182_23155"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX6440035.1"
                     /translation="VGLTPYGIFAIAANTAGTLNLEQVARIQIYLITYMTIALLLGLW
                     VLPGLVSALTPFTYRDVLGPTRDALITAFIAADLFIVLPILIQACKGLLERYGLVNET
                     TKSLPDVIVPTSFNFPHSGKLLSISFILFAGWFADASVPLRQYPHLALMGVLTLFGST
                     TAAVPFLLDVFRIPADTFQLFLATSVVNVRFGALLAALHTIAVALLGSAAIVGALRFD
                     LRRIARYLVVTVALSSVALVGLRAVFSTVLKPTFSGEEIIFNMGPLLQLAETQRINEA
                     DLRDPVIRESDSTVQRIKRRGALRVGVVANRLPYVLADREGKLVGLDVEMAQVLGRDL
                     GVEVEFIELEGLTALLPLLSSGRIDLAMSGIAVTPERAGEMLFSDPYLDETLAFVVKD
                     HLRDQFGSWTEIRELGSFPVALPNLPYYIDKIKSRAPALKLAPLDSTNQIEDSLKRET
                     FEAVVMPAESGSVMSLLYPKFTVVVPEPEIVKIPLAYPVARRDQDWAHFINTWIELKR
                     RDGTIDALYGHWILGKQAGKRQPRWSIMRNVLHWGD"
BASE COUNT         2875 a         3825 c         3718 g         2736 t
ORIGIN      
        1 tgctccttgg cgcgcgccag ccagttcatc gcaatggggt gatcctccgc cggatttccg
       61 cccatgatca gaacgacatc ggcgttcagc atatccaccc aatggttggt catcgcccct
      121 ctgccgaatg aggccgccag accggcgacc gtggagctgt gtcagagccg ggcctggtgt
      181 tcgacataca ccaggccgag ggccctcatg agttttacga ggaggtaggc ctcctcgtta
      241 tccaatgccg agccgccgag acaggcgatg gcttcggtgc gatttaccac ccgcccgtcg
      301 atcgttgtct tgaaggtttt atcgcgcgtt tctttgatgc gcgcggcgat ccgctccagc
      361 gcccaatccc agctcttctc ttcccacgcc gcgccgttag gtttgcggta caggactttt
      421 tgcagccgcc gcgagttgtt caccacctgg atgcccgccg ctcccttgct gcacaaagtc
      481 ccttcgctga tcggatggcc agggtcgccc tcgatgtgga tgaccttgcc tgcccgcgaa
      541 gagacgacct ggccgcagcc gacaccacag aacgggcaca ccgtagtggt attggtagcg
      601 ccgcgggttt tgagcggcgg aaccgccggg ccgcagcccg actgcgtcag cgcgccgatg
      661 gctactgtgc cggtgccgag ccccgtgagg aattggcggc gcgttacttg tgcgggcgcg
      721 ctgcctgctc tatcatcctc gccgttccgt tccgggttat cttcattttc atttgtcatt
      781 tggccctcga tcccatgcgc gttgttgcgc ttgtttctaa tggatgcgcc cggcttatct
      841 ttgactatct tgctagcggc tgacgatctc gatccttttg cgatcgcaga ggagaaaact
      901 tcgcagctgt cgatctgcgc ccgtggatct cggtagtcgc agatcgctct actcgtgctt
      961 ggaatatcag aaggcagacc agtcaggtat tggaccttgg tccaataggg catttctgag
     1021 taatcgcagt tcaatgccgg tgaaaagccc tggagaacat cttaagttcg gttcaagacg
     1081 ttcaaccccc atctcgtttc gttcccgcga tgcggcggag gataatcccg gattccgcag
     1141 cagagatgga tagctgaaga gcgatcgagc ggcgggcgtg gaggagcgaa ggcatagaag
     1201 cgaaccgttt caatgctggt gcttggccct gggctacaaa ttttttcaag cgtccgctaa
     1261 agcactggag cttgtagggc cgctatcagc caagacctat gccttcgttc cggagggttc
     1321 gttctttgat cgctgtgtca ccctgaactg cgaaattcgg gataataaga gaggttgcac
     1381 cgaatcaaac atctgtaaag actcccgcat tgtcggtgac ctcccggccg ctgttcggtt
     1441 taattcccgc agttaccaag tgtgcttcac ggtttgcccg tttgagcgcg cgcttcgctt
     1501 ttgccgtctc gcaatacgat ttgacctttc ctggagaaat caacggactc gccgagaatc
     1561 cgcgccgcct cttgcggcgc gtgaaagccg gtcgagtttt ccgcctcgac gaaatcgaga
     1621 taaaattgcg ccttgcgctg gaagtcctgc gctgctgcaa gctccttgtc ggtcttgccc
     1681 gcgctttttg ccgctttgag gtctttgatc aaatccatca acgcatccat tgccacgttg
     1741 cgcatcttga atgtgcgctc ctgaatcgtc tcgacccgct gcttgagttc ttcctcgggc
     1801 cacttatgac acgtctggca ggcgcgattg atattgagca ccgggctgcg cacatgatgg
     1861 tcgctgatct tgagcgcgcc ttcgcgtttg tacggcatat ggcagtcggc acaggtcacg
     1921 ccggaacgcg cgtggatgcc ttgattccac atctcgaact caggatgctg cgcctttaac
     1981 accggcgcac cggtgtcggc atgcgtccag tctttgaatt gcgcttcgtc gtaaaaggct
     2041 tgaatgttct ctaccttgag tcctttcgcc cagggataaa cgaggcgctt ttccggccct
     2101 ttgaaataat attcgacatg acattggccg cagacgtacg cgcgcatctc ctggcgcgtc
     2161 gcctgcttgt tgacgtcgta gttctgaatg ccttgggacg ctttaaaggc agcaatgcct
     2221 tcgataaaac ctggccgtgt aacccgcagt tgcatgttgc ccggatcgtg gcagtcgatg
     2281 caggccaccg gatgcgagac cagtttgcgc gcttcgaaga acggcatttg attgaacttc
     2341 tcgaaacctt tgatgagatc gccgtccccc gccttcttgt aaggcacgta gaccgacccg
     2401 tggcattgaa tgcacgtgcc gggctgctgc accacttttt ggcgttcggt caaggtctgg
     2461 tcatcgagca tgtaagcgtg gccgcgctcc tcgcgaaaat ccttggcaaa ggcgtatccg
     2521 gcccacatcg tctttaaacg ggggtcttct tcgatcttcg actgggcgac caccgagcgc
     2581 ggatcggcct ttgtcggcgt atgcgggagc gcttcgctgc caccatagcg tgtgcgcacc
     2641 tgatccaccg ttcgtttgta gtcgtcgtac tgcagcggga agttttttcc ccagaccgcc
     2701 ggatcttccg tctcgtcggt caactcgaca acccgataaa atggattgcg cgcctcctgc
     2761 ttgcgctcaa agatattcat cagtaggccg accccgacga tggtcgcgag caaagccgcg
     2821 ccgacgatga tgaaaaataa acggcgccgc cgccgagtgg aggcatcgag atccctagcc
     2881 tcagccatgt ttcctcccta atgggtcact catcgaattt cggatcatca gtggagatga
     2941 cccacagagt tatgacaact caggcaagcg agttgcccgc ctgccgatga cagatgcgac
     3001 ggttcaattg catcgacgat ttctccatga cagccgcggc aagcccgctc ggtgatttcg
     3061 cggctgtgcg gttttatctg aatattgtcc tcgtagccac ccgtggtgaa gaaaaacgaa
     3121 tgccagaagc cgttcgatgc tttcgtcgca tatttgccga tgaagttgtg cggggtgtga
     3181 cagctattgc acgtcgcaac cgagcgatgc gatgacttga tccaaccatc gaattgctcc
     3241 tgcataacgt ggcaattggc gcacgccgcc ggatcatcag taaggtagga ccaacccttg
     3301 gcataggcga aggtgtatgc gccgatgcca atggcgacac ccgcaatgat tccgagcaca
     3361 atcgcgtaaa tcggggcaag cttcatctgc tgttacggca atactcttta cgctgacgct
     3421 aacgagctgc gcaaagcctg tctattggac tttggtccaa tcactcacac aggaaaaggc
     3481 tttttaagtt ggaaagtgat agaggaggca tttcggcaat cagtgtgtct ctggttggca
     3541 gcgtgaggga tgttatccgc cgctatgcct acgatttggt tttcggttcc gcttttacgt
     3601 acaagctcac cagacgagcg accatcaccg caagaaaaag ctgcccgccg acgccttcga
     3661 cgatcgccaa gccgcgcaca acgtcgctgc gcggcacgat gtcgccgtag ccaagcgtcg
     3721 ccagcgtgac aaaactaaaa tagaacccgc tcatgcgcgt gaagtcagcg gcggcggtga
     3781 agcttcccgg ttccgtttgc tcgactaccc agtagaacaa accgaaaaaa atccctgcaa
     3841 gcatataagc gcttagcgct gcgtaaagat gctctgcatc gatcggctcg ttgtccagcg
     3901 caaaccgcaa agcccccgcg gctccgaaca gcgccagcac ggtccacaaa ccgagcgtca
     3961 tgaccgacag cgcggaatga tcgagccaag ctgttgcggg ccgcgccagc caaaccagga
     4021 ctgtagcgac gaacagaatg cgacggctcc taaccgttgc gaccggaatt agcgcggcaa
     4081 gaaggttcgc cgccaggaac agttcaataa gaacgccttt gaattcgaac gcactcaaaa
     4141 ggggagcggc gacgagagtc agaaaaagag tgtagaacag aatcgtgtag cgccgatgaa
     4201 agtaggcgcg ccagaaatcg cgcagccagc ttgcgcgttg cgcgctcact tcgctcatcc
     4261 cggcatgact tcgggcataa agtaaaacaa gaacgccatg atggtgtaca cgatgatgag
     4321 ctgaacgccc ttgtaccagt tggcgcgccc gtcacctgaa acaatggcgc caatcaatac
     4381 cgcaagaaac atcgagccga gctcggctcg tccaaacgag agctcgagag gctgcggcgc
     4441 aactgcatag ctcaaaagca ccagcgcagg cgcgacaaag agcgcgattt ggatacaact
     4501 gccgagcgca atactcaccg ccagatccat cttgtttttg cgacccatac tgatcgctga
     4561 accgctctcg gcagcgccac cgacaatcgc caaaaaaata atcccgataa acatctgtga
     4621 catgccgaga gccttcccgg tgccttccgc ggcgccgacc aagatctcgc tcatccacgc
     4681 ggccagcgcc gatgccgcaa gcaaactggc gatcgctcgg ggcagtcccc actgttggcc
     4741 gacatgatgg tcttctgcct tggtgtctga gctggaaaaa aactcaggat gagtcttgag
     4801 catgaaaacc aggtaaagtg cgtaggcaat taaaagaacg accaccatac ctagattgag
     4861 aagctgctca tcgcgtacga tcacgtctga ggaaaacaaa cggctgaacg cgctcggtac
     4921 cacgagactg atcacagcga tcagcatcat cgagctgtag actcgcgccg ccgccgggtt
     4981 ataggtttga tcgtgataac gcagtccgcc cagaaagaat gccactccta aggcgagcag
     5041 gagattggcg agaatggctc cgatcagcga ggcccggacc atctcgaaca gcccggcctt
     5101 caaagcaacc accgcgatga tcagctcagg ggcattgccg aaagtcgcgt tcagcaaccc
     5161 tccaaccgca tcgccggtcc gcaccgcgat gtgctccgtc gcgttgacga tgaggcttgc
     5221 gaccggaata atcgccacgc aagcggcaaa aaagatccag gtgtgcgcct gaggcgacgc
     5281 atgctcgagc gccaccgcaa ctggcacaaa gataaaaagc caattcattc tcacggtgaa
     5341 ttcctgcgga tggtagatgc gctgctctgt cgggaaaaac gatttcagag tattggaata
     5401 gtgaaattcc aaatgcatcg gctccgtact acatcactca cctctccaat gctctgcgcc
     5461 attgacaacg ctcgcatgct ttgacggttt gaccttttcg gacggctccc aacccattgg
     5521 aacttattct tacggctgcc aaccgccgcc gagggctcgg tacagttgca ccacactatt
     5581 gagctcagcg ccgcgggtgc gtgcaagttc caattcggcg tcaaaaaggt cctggtcagc
     5641 gatcaaaact tcaagatagt tcgccagccc cgtgtcatag cgcaaccgcg agaggctgga
     5701 cgcatcctgc agcgccgcaa cctgcgcttc ctgctcggca cgcacgtcct tgaatttttc
     5761 aatcgctatc agcgcgtcgg caacttcccg gaaaccgttt tgcgccgcct tctcgtattg
     5821 agccaacaac tggtcaaaac gcgctatcgt cgattcgtag ttccagaata tccgccagcc
     5881 ctgaaagatc ggttgcagga tgccaccgga caccgaccag atcgccgcgc gcttgtcggc
     5941 gaggtcggag agatcgtgac tggtgccccc cagcgctgcg ctcagcgaga agttagggaa
     6001 gtagagagct ctcgccgcgc cgatgttggc attggcggca accaataact gttcggcgct
     6061 ttgcacgtcg ggtcggcgct cgagcaaggc tgccggcaag cccgccggga ccgttggcgg
     6121 ataatattga gctgttaaca ccgggccgcg cggaatcgtt ccagggttac gcccgagcag
     6181 aaaattgatg aggttttctt gggtaacaat gcggcgttcg agatccggga caaccgcggc
     6241 ggtgcgcgcg cgattggcaa gcgcctggtt gagttcgagc cggttggaga cgccgccatc
     6301 aagccgcttt cggtaaaatt gagtggtctc gtcgttaagc tggagcgtgc ggcgcgtcac
     6361 ctcgagctcg agatcgagct cacgcaaatt gaagtaagct tgcgcgacat cggcgatcag
     6421 cgcaatcaac acgccgcgcc ggtccatttc ggtggcgacg tagatggcgg tggccgcttc
     6481 ggtttggcgc cggatcctgc cgaatacgtc gatctcccaa accatgttga agaccccgaa
     6541 ccagttttga aatgatcgcc tgctgccaaa agattcgggg ggatcggtca gtcccgagac
     6601 ctgttgcgcg ctgccgccga cgtttgcgtc gaatcgagga taaagaaaag agcgggtcac
     6661 gccgatctgc gcccgcgctt cctctacgcg cgcagcggcg gtgcgcagat catagttgtt
     6721 gcgcagggcc tctcgggtca agtcctgaag cacgttgtcg ttgaaaagct cccaccaggg
     6781 aatgtccgcc aaagattcag ctttgggcgg ttccacggcg ccacgatggt tatccggaat
     6841 gggcactccc ggccgcgtat aatctggacc gagcgtgcac cctcctatga agaaggcgag
     6901 tatggcggca agcgcgactg ctctcattta gtgtgccccc tcatgggagg gcaccaccgg
     6961 aattgcggcc ggcgctaatt tcttctttct ccccagtcct tcgacgaacg caaagttgcc
     7021 ggggatcagg aacaccccca gaaaggtcgc taccagcatg ccccagaaga cggcggtgcc
     7081 catgacattc tgcgctccgg cgcctgcgcc gctggcgatc atcagcggca ccacgccgag
     7141 gataaacgca aacgcggtca ttaaaatagg ccggaagcgc agacgcgccg attccaaagc
     7201 ggcctcttcg agtgacatcc cttcttcatg ctttgccttg gcgaattcga cgatcagaat
     7261 cgcattcttc gccgccagcc cgatgagcat gatgttaccg atttgcacgt agacgttgtt
     7321 gtcgtagccc atcagccaca cgccgaagaa ggagccgaga gccacgaggg gcgagccgag
     7381 gagtactgcc caaggtagcc gccagctctc gtacatcgca gcgagcagca gaaagacgaa
     7441 aacaatggcg agaataaacg tggggcccgc gggcggcgcc accttttcct gataggaaag
     7501 actcgagtag gcgaagccca tctccttcgg catggtttgc ttgaaaacat cttccagcgt
     7561 cgccagcgcc tgtcccgaag tgaacccacg gccggggacg ccgttgattt cgaccgagcg
     7621 gaacaaattg aagcgattgg tgacttccgt gccactttcc ggggtgatgg tcaccagcgt
     7681 agatagcggg atcatgcctt tcgtggtctc gctacgcacg taaatatcgc cgatgtcttg
     7741 cggtttacgc cggtagtcgg catcggcctg gacataaacg cggaacaagc ggccgaaacg
     7801 attgaagtcg ttcacatagt tgccgcccat gaccgccgag agcgcctgga acacttcatt
     7861 gacgggaaca ccgagtttgc gcgctttttc tcggtcgaga tcgaccttga tttgcggata
     7921 gcgcgggtca aaggaggtga agagattggc cagctccgga cgtttgcgcg ccgcttcgag
     7981 aaattgtcgc gataagtcgc cgagctgctg cacgctgagc gatccgctcc ggtcctgcag
     8041 cagaaaattg aagcctgccg aagcgccgaa cccggagatc gtgggaatgt tgaaaggaaa
     8101 tatgatcgcc tcgggaatat tcgcgagctg ccgctgtaaa cccgccatga taccgcgcac
     8161 gtgcagcgcg tcggaatgac ggtcgtccca tggtttaaga cgaacgaaga tcgtgccgaa
     8221 attaggctga taggtgctgg tcacgacgcc gtatccaccg atcgtcgtga acgagtcgac
     8281 gccttctatc ttggtcaaaa tctgctcgac ctgcgcgagc acttttcctg tccgttcgag
     8341 agaagcggcg ggcgggaggg tcacgttgac gccgaagatt ccctggtcct cttccggaat
     8401 aaagccggcc gggagcgccc cgccgaagag gcccgccccg actgccactg ccgcaatgat
     8461 caggatcgtc aaaattgacc gccgcaccag tagccgggtg gtgttcacgt aaccagtggt
     8521 tgagcggtcg aaaacccagt tgaagccgct gaagaatttc cccagcgggc cgcgcgccgg
     8581 cttgggcgcg cgcagaaaca tggcgcagag cgccggcgac agcgacagcg cgttaaacgc
     8641 cgaaaggaga acggagatgg cgatagtaag agcgaactgc tggtacatgc gcccggtgag
     8701 accgcccagg aaagctaccg ggatgaacac agcggagaga ataagcgcga tgccgatgac
     8761 gggcgccgac acctctttca tcgcctgtat agttgcctcc ttcgggttca tgccgtgctc
     8821 caggtggtgc atcacggcct cgaccacgac gatggcgtca tcgacgacga taccgatcgc
     8881 gagcaccagg ccgaacatcg aaagggtgtt caccgagaag ccgagcagcg ggaaaaagat
     8941 gaacgtgccg atcaacgaca ccggcaccgt aagcagcgga ataaacgtcg cccgcaggtt
     9001 ctgcagaaag atgaagacaa ccagggtgac taacacgacc gcctctataa acgtatgaac
     9061 gatgccgtcg atggacgctt tgaccgccgg tgtcgtgtca tagacaatct tgtaatccat
     9121 atcgggcggg aacaacgctt tagcattgct catggtctgg taaattgcct cggatgcctg
     9181 gagctggttc gcgcccggta gcaagtacac cgccatggtg cccgccgcct tgccgtcaag
     9241 acggccaaac gagttgtagt cctgtgagcc gagctcggcc cgaccgacgt ctttcacgcg
     9301 aatttgcgcg ccggttgagg attcgcggat aatgatattt tcaaattcct cgggattcac
     9361 gagccgtccg ggcgcgctca cggtccaggt aaattcctgg tccttgggcg atggcgccat
     9421 accgacgcgt ccggccggcg cctgaagatt ctgctcttta atcgcggaga tgacatcggc
     9481 cggggtaaga ccaagtctgg cgagcctgtc cggtcgcagc cagatgcgca tgccgtagtc
     9541 ggcgccgccg aaaagatcca cctgcgcgat accgggaata cgcaggatct ggtcacgcag
     9601 gttgatacca gagtaattga ttaaaaagtt tgcatcatag ctttccttgg gcgagtacaa
     9661 agagatcagc atcaggatgc ttgggctctg cttcttgacc gtcacgcctt gctgcacgac
     9721 ttcctgcggc aaccgcgctt gcgctgatga gactcggttc tgagtcaaca cattggccgt
     9781 gtcctgatcg acgccgacct cgaagttaac gtcgagctgc atgcgcccgt cgctggtgtt
     9841 cgaggatttc atatagatca tcttgtcgac gccgttgact tcctgctcga tgggcgtcgc
     9901 caccgattgc tccaccgcta ccgccgaggc tccgggataa gtcgccgtaa cgcggatagt
     9961 cgggggcgcg agaaacgggt actgctcgat gctgagtccc ttgagcgtga aaagacccat
    10021 gagcaccatg atgatcgaaa tgacgatggc aacgatgggc ctacgaatga aaaaattagc
    10081 cattgcctct acttcccttt cgtttccgcg ggattgccat ccattggcgg cggcttctga
    10141 ccgaacgcat ctgcggcttc gctcttctcc agttttgcgc tgactttcac gccggggcgc
    10201 actttttgaa tgccttcgac gataaccttt tcacccggct tgactcccga atcgataatc
    10261 cacaagttgc cgatccgttc gccggctttc agcatacgct gctccaccgt ctcatccggc
    10321 ttcacaacca tcacgctgta gaggccttga atctcctgca cggcgcgttg cggcacgagc
    10381 acggcctctt gcttaacctc ggtgacgata cgcgcgcgtc cgtactggct cggacgcaga
    10441 attcgctcag gattgggaaa gctaaattgg ccgctcagag ttcccgtcgt tggatccacg
    10501 gcgcgctcga tcgagtccag acgtccttta tgagggtgca cgcttccatc ggcaagaatt
    10561 aactcaacgc cgaatttctg gttctgagtt ttgtcgcgac cggcgccgcg ccgagcgagt
    10621 cttaaatatt ccgcttcggc aatcgcgcag cgaaacagaa tgggattgat ctgtgaaaca
    10681 acattgagca aagtctcgcc tcccgaaatc aagctgccga ctttcttctg ggtcgtgccg
    10741 atcaggccgt cgatgggcga agtgattgtg gtgtaggcca ggtcgagctt agccttggcg
    10801 actttggcat cataggccgc gacctgagcc tgggaagcct cctgcgccga cagggcattg
    10861 tcgagctcct gttgactgac cgcctgttgc tttgcaaggg gcgttagacg agctacgtca
    10921 ttattggttt tgtcaactcg cgcgcgcgac gtcgcaaggt ttgccttggc ctcattgagg
    10981 gcagcttcaa atggctgggg atcgatctta tataagagct ggcccttctt gacgaatgtg
    11041 ccctcagtga agttcatgga aaccaggtaa ccttccacgc gcgcgcgaat ctcgacgtcc
    11101 tccgagccca gggtggcgcc gaccagctcg atgtaaatcg gcacgtcttt ttgtacgacc
    11161 tcggctactt ggacttcggg cggagccggc ggcggcggag cctccttctt cgagcaggca
    11221 gccgccagga caaggcaggt catgagaacg gccaaaattg ccgttgatcc cgttcgtgtc
    11281 acgccatgaa tatttcgatt cacgttatcc cccaagcttt gaacgcgatt tgcataatga
    11341 tttgcatgcc tcggcgcctc ttcttcccct gcgaagttag ttatgccagg aattagcagc
    11401 gctcccgacc ggcgatgatt cattatcgaa atcttctaac accgtatatt ggaccttagt
    11461 ccaatgatgg tgcaggagag agagatgacc tacgaacagg acccgcatct tcaatacaat
    11521 cagtcccccc agtgcaatac gttacgcata atcgaccagc gcggctggcg cttgccggcc
    11581 tgcttgccga gaatccagtg gccgtaaagc gcatcgatag tgccgtcgcg ccgcttcaac
    11641 tcgatccaag tattaatgaa atgcgcccag tcttgatcgc gccgggccac tggataagct
    11701 agcgggattt tgacaatctc cggttccggc acgaccactg tgaatttagg atagagcagg
    11761 ctcatgaccg agccgctttc ggccggcatg accaccgcct cgaaggtttc tcgtttcaaa
    11821 ctgtcctcga tctgattggt ggagtccaac ggcgcgagct tcagtgctgg cgcgcgcgat
    11881 ttgatcttgt cgatgtaata cggcaagttg ggcaaagcca ccggaaaact accgagttca
    11941 cgaatctccg tccagctccc aaattgatcg cgcaggtggt ccttcaccac gaacgcaagc
    12001 gtctcgtcaa gatagggatc ggaaaaaagc atttcgcctg cgcgctcggg cgtcacggcg
    12061 ataccgctca tggcgagatc gatgcggccg ctggagagca acggtaataa cgccgtaaga
    12121 ccttccagtt cgatgaattc aacctcgacc ccgagatcgc gcccgagaac ttgagccatc
    12181 tcgacatcga gcccgacaag tttgccctcc cggtccgcca gcacataggg aaggcgattg
    12241 gcgactacac cgacgcgtag cgcaccccgc cgcttgatcc tctgaaccgt tgaatctgat
    12301 tcccttatga ccggatcgcg caggtccgcc tcgttaatgc gctgcgtctc ggcaagttgc
    12361 agcagcggac ccatattaaa aatgatctct tcgccgctga atgtcggctt caacacggtg
    12421 ctaaagacgg ctcgcaggcc gacaagggct actgatgaca gagccaccgt aaccacgaga
    12481 tagcgcgcga tccgccggag atcgaagcgc agggcgccaa cgatggccgc gctgccaagt
    12541 agcgctaccg caatcgtgtg aagcgcagcc aacagagccc cgaagcggac attgacgacg
    12601 ctggtagcca aaaacaattg aaaagtgtca gcggggatgc gaaaaacgtc caacaagaac
    12661 ggcacggccg ccgtcgtgct gccgaataat gttaggacgc ccatgagcgc aagatgcgga
    12721 tattggcgca acggcaccga tgcatcggca aaccatccgg cgaatagaat aaaactgatc
    12781 gataaaagct ttccgctgtg cgggaaattg aacgaagtcg gcacgatcac atcgggtagg
    12841 cttttggttg tttcgttgac cagtccatag cgctcgagca gacccttgca tgcctggata
    12901 agaattggca gcacgatgaa cagatcggcg gctataaatg cggtgatcag cgcatcgcgg
    12961 gtcggcccga gcacatccct ataggtgaac ggggtgagcg ccgaaaccaa tccaggcagc
    13021 acccacaaac ccaggagcag agcgatggtc atgtaagtga tcagatagat ttggatacgc
    13081 gccacctgtt caaggttcaa tgtcccggcc gtattggcgg caatggcgaa gatgccgtaa
    13141 ggcgtcaagc cgac
//
LOCUS       DASUVS010000440         8459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1067842, whole genome shotgun sequence.
ACCESSION   DASUVS010000440 DASUVS010000000
VERSION     DASUVS010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8459
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067842"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..790
                     /gene="msrA"
                     /locus_tag="VF182_23160"
     CDS             230..790
                     /gene="msrA"
                     /locus_tag="VF182_23160"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX6440036.1"
                     /translation="MTQQKEVAVFGGGCFWCTEAVFDELWGVTSIVSGYAGGNTKNPT
                     YEQVCGGHTGHAEVIRIEFDPGQIAFADLLTVFFATHDPTTLNRQGNDVGTQYRSVIL
                     YADEEQKRQAESFIRELEAAGTFSRPIVTTLEPLTEFYEAEGYHQKYYANNPYQPYCQ
                     VMIPPKLSKLHKQFGELLKSHSKGGS"
     gene            1212..2981
                     /locus_tag="VF182_23165"
     CDS             1212..2981
                     /locus_tag="VF182_23165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440037.1"
                     /translation="MISGRKFFLGCAIAAAIALLVGRIVPLPVSLLAGEVFATILGLF
                     LFGSFRYQIHKNAITYGMALIIIATFCGLSSSQWHVEIAQRGWGDWAQEHLFSLHGLD
                     DLIHADTMLFILGLTFFVAVIAQTRLLEGLTFFLLRRYRGAVLPTALSVTAVVALASG
                     ILDGVSMIGLTIRTLVIILLLAAAPTAAIRYAVMVCTAVTTVCGIWLAYGEPPNLIMK
                     ANLHPYIDNRFFLRYCAPIAIASYLVIAWQLRRRLRGEHVNLETMDVLDANAQDVRFL
                     QAMRHGEVLTPIEFVENHAGELGDKTESVMERLREGESVGLALVREEITEVTRRELLG
                     RFVSEDLADPLDRHYVLQAAGDQAGAVAAQRQVDHTLAASARRRKIAQRVGALALIPF
                     VGMLVWHGLDHRVPLFLASFAGFFVALLGIAAIPKMRALSLTEARQEYAEYYFLFPLF
                     LSITLLTEAGFFDRIQYLIRHSIASLGRSHVAFAQFIGSSFLSAILDNNVVADFASRA
                     LHGLPIDVMHLFAMAQIAGYALGGCWTHIGSAQSVVAYAFIRRDIDEDYTPVQWIKEM
                     TPIIVQLLLLITVLIYVEGALFS"
     gene            3146..4219
                     /locus_tag="VF182_23170"
     CDS             3146..4219
                     /locus_tag="VF182_23170"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX6440038.1"
                     /translation="METDTQQIALVDHHKLTRFVATAFERLGVPSPDAAIAADVLVMA
                     DLRGVDTHGVIRFNPRSWYVKWLREGEMSATPKIQIVSEAGSSALIDGGRGIGMVIGH
                     RAMELAIEKAKLSGAGMVGVCNSRHFGMSAYYAMQALPYDMIGIAMTNAGRQVVPTFG
                     SEARFGTNPICFAAPAQNELPFVIDMATTTAAAGKLELAVRRGASIPLGWALDEKAEP
                     TSDPKIAQRARRLLPLGGSREGGSHKGYGLAILVEILCGVLTGTVTALNQNQDPRGHF
                     FAAIKIDSFRPVSEFKRDMDRLIHQLKATQPIAGQDRVYIAGEIEFETAKRRAQEGIP
                     LLGSVIKGLREVGEQLEITCDLE"
     gene            complement(4248..4793)
                     /locus_tag="VF182_23175"
     CDS             complement(4248..4793)
                     /locus_tag="VF182_23175"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6440039.1"
                     /translation="MAAQTTDKKIPVYATFKTSMGDIVVELFEDKAPKTVANFIGLAS
                     GTKEWTDPKTGEKVKRPLYNGTIFHRVIPGFMIQGGDPLGNGTGGPGYRFEDEFHPEL
                     KHNKSGILSMANAGPNTNGSQFFITHKATPWLDGKHTVFGEVIKGQDVLDAIAKVGRD
                     SRDRPTKEIVLQQVIISRGSY"
     gene            5189..5689
                     /locus_tag="VF182_23180"
     CDS             5189..5689
                     /locus_tag="VF182_23180"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX6440040.1"
                     /translation="MPTPSTLEPDPPPVAVTPKSKIESAVSPSEPRLVGHLHKGSRIT
                     GQLSFEGSVRIDGSVEGEIRCLETLTIGEGAEVRARISSRVVIVRGKVEGNVTAKDKV
                     ELLAPGRLFGNVSTPRLIITEGVVFDGDCSMGAAKEGGVVGSRTLNAEKVAVAQSSKL
                     QPDSNK"
     gene            5834..6262
                     /locus_tag="VF182_23185"
     CDS             5834..6262
                     /locus_tag="VF182_23185"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEX6440041.1"
                     /translation="MIALNISVLWQILLFLGLWLIVSKVLFRPYMTVLEQREQKTTGA
                     DDSSEHLEQQAERLRAQYEETIVNAAAAGNASKNAIVQQARQQREALLTSAREEAAGI
                     LERVRQEVQSQLAQEHELAVREADAVAQDMVSKILGRRVG"
     gene            6259..6804
                     /locus_tag="VF182_23190"
     CDS             6259..6804
                     /locus_tag="VF182_23190"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440042.1"
                     /translation="MNPRAWFLVAEAWAAGGGEHHPPEISGIIFPLLNFLIYAGIIYY
                     FAFPLIRSFLKARHDEVLATVTEAAARKRSAEALVADYESRLANIDQEIDSIQASLKA
                     EGERDKARLLREAEGLATKIKSDAQLLADQEVKAAKHQVLLELAERAKVSASDLIRRH
                     LSRADQGRLVEEFIDDLGQIR"
     gene            6801..7343
                     /gene="atpH"
                     /locus_tag="VF182_23195"
     CDS             6801..7343
                     /gene="atpH"
                     /locus_tag="VF182_23195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX6440043.1"
                     /translation="MIEDSLSRRYSKALFELARESGQEESIAEQLERFSSTSTESRLG
                     AVLSNPGFALDSRKKILLEVAKSLQLSSITEHFLSLLLDRDRLAFIPSIIASYRHLLN
                     QLKGRVDAKVTAAEPLGAIAIERLRDSLRRLSGKDVILQEEANPELIGGLVVEFQGKI
                     YDGSVRTQLETMKQRIARGF"
     gene            7354..>8459
                     /gene="atpA"
                     /locus_tag="VF182_23200"
     CDS             7354..>8459
                     /gene="atpA"
                     /locus_tag="VF182_23200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX6440044.1"
                     /translation="MEIGTAEISRIIKEQIRDYEKTVEVQEIGTVLSTGDGIARIYGL
                     DRVAAGELLEFPHNIFGLTLNLEEDNVGAALFGETHMIREGDTVKRTGRIAEVPVGKA
                     LVGRVVNALGEAIDGRGPIETSDRRRIEIKAPGIVARQPVKEPLQTGLKAIDAMIPIG
                     RGQRELIIGDRQTGKTAVAVDTIINQKGGNVICIYVAIGQKRSTVAQVVDRLREAGAM
                     DYSIVVAATASDSAPLQYIAPYSGCTIGEYIRDNGGHALVIYDDLSKHAVAYRQLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKMSDAMGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIFLESDLFYSGVRPAINVGISVSRVGG"
BASE COUNT         1923 a         2162 c         2363 g         2011 t
ORIGIN      
        1 tcggcacggc ccagaagagg tttaccgcac tcagtttata ccagtcatcg catcactgcc
       61 gtagtacatt ctaaaacgaa cgtggcagcg gaattgccga attgccgttc agaacggtat
      121 ggggcgttaa gcccgaagga gcaacgcagt gcgtgaagct tatcgtttcg cttgcttgcg
      181 aaagtgagtc ggccgctaca tacgatagta gcaagggagt tggggcacga tgacacagca
      241 aaaagaagtc gctgttttcg gcggcggatg tttctggtgc actgaagcgg tatttgacga
      301 gctctggggc gtgacttcga ttgtctccgg ctatgctggc ggaaacacga aaaatccgac
      361 ttacgagcag gtctgcggcg gtcataccgg acacgcggaa gtgatccgaa tcgagttcga
      421 tcccgggcag atcgctttcg cggatctgct gacggttttc tttgccactc atgacccaac
      481 gacacttaat cggcagggca atgatgtggg tacgcagtac cgctcagtca tactttacgc
      541 ggacgaagag cagaagcgac aggcggaaag ctttatcaga gagctagaag ccgcgggcac
      601 ctttagccga ccgatcgtga cgactttgga accgcttact gagttctatg aagctgaagg
      661 atatcaccag aagtactacg ccaataatcc gtaccaaccc tattgccagg taatgatccc
      721 tccgaaactg agcaagcttc ataaacaatt cggcgaattg ctcaaatcgc acagcaaagg
      781 cggaagctaa acgttcgagc tggaaggagt cgcgagtcgt catacagtcg agcagtacag
      841 gcgccacatt ccagcgctgt gattttcttc gcggcagatc ctcgtgaatc tgggtcttgt
      901 atcaaaccgc cgtatgttga tctttacgct tgtcgcttcg gcgaggcgtc ttcactcagg
      961 tgtggtaagt tccaaaatta aagcctacga tttcgcagcg atttgaatcg tgcagatcgc
     1021 ttcgtcgcac agaggtcatt acaaagccat ctcagcgtga cattcgtgaa tagacaacga
     1081 tgatcctgaa gatggttcga acgcggctct cgcagaagcc ggtctagctt gaaactaacc
     1141 gagcgtgcat cggtgaactc ggatcttaag ctgacaagag aattatgccg gtccaaagct
     1201 cagcagtaat aatgatctcc gggcgaaagt ttttcctcgg ctgcgctatt gcagcagcca
     1261 tcgctttgtt ggtcgggcgg attgttccgc tgccggtatc gctcttagcg ggggaggtct
     1321 ttgccaccat tctaggccta tttttattcg gctctttccg ctatcaaatt cacaaaaacg
     1381 caatcactta cggaatggct ttgattatca ttgcgacatt ttgcggtcta agcagctcac
     1441 aatggcatgt cgagatcgct cagcgaggct ggggggactg ggctcaagaa cacctctttt
     1501 cgttacatgg cttggatgat ttgatccatg ccgacacgat gttgtttatt ctcgggctaa
     1561 cgttttttgt cgcggtgatt gcgcaaacac gtctgctcga aggtctgact tttttcttgc
     1621 tacgtcggta tcgtggcgcg gtcctcccga ctgccttatc ggtcaccgca gttgtcgccc
     1681 tcgcctcggg catcctggac ggcgtctcga tgatcggttt aaccattcga acactggtga
     1741 tcattttgct gcttgcggcg gcgccgacgg ccgcgattcg ttacgcggta atggtttgca
     1801 ctgcggttac gacggtgtgc ggaatctggc tagcctatgg cgaaccgcct aacttaatca
     1861 tgaaggccaa tttacatccg tacatcgaca accgcttctt tctgcgctac tgcgcgccca
     1921 tcgcgattgc cagctacttg gtcatcgcct ggcagctgcg cagaagattg cgcggcgagc
     1981 acgtcaatct agaaactatg gatgtgctcg acgctaacgc ccaggacgtg cgttttctgc
     2041 aagccatgcg gcatggtgaa gtgttgacgc caattgaatt cgtcgagaat catgcgggcg
     2101 aattgggcga caagaccgag tcagtgatgg aacgcttgcg tgagggcgaa tcagtaggcc
     2161 tcgctctggt tcgtgaagag attaccgagg tgacgcgcag ggagctcctc ggtcgttttg
     2221 tcagcgaaga tcttgctgat ccactcgatc gccattatgt acttcaagct gcgggagacc
     2281 aggctggggc ggtcgcggcg cagcgccagg tggaccatac tctcgcggct tctgcgcgac
     2341 ggcgcaaaat tgcgcaacgg gtcggcgcat tggctttgat accctttgtc gggatgctgg
     2401 tttggcacgg actggatcat cgggtgccgc tatttctggc ttcgtttgcc ggctttttcg
     2461 tcgctttgct gggaattgca gccatcccga aaatgcgcgc cttgtcctta actgaagcca
     2521 gacaggaata tgctgaatac tattttctct tcccgctttt tctctccata accttgctga
     2581 ctgaggcagg ctttttcgat cggatccagt atctgattcg ccacagcatt gcgagtcttg
     2641 gtcgatcgca cgtggccttt gcgcagttta tcggaagctc tttcctgtca gccatactcg
     2701 acaacaacgt cgtcgccgat ttcgcctcgc gcgcactcca tggcttgccc attgatgtga
     2761 tgcacctgtt tgctatggca cagatagccg gctatgcgct gggtggttgc tggactcaca
     2821 tcggctcagc acagtcggtg gttgcttatg ccttcatccg ccgcgacatc gacgaggatt
     2881 atacgccggt gcagtggatc aaagagatga ctcctattat tgtccaactg ctgttattga
     2941 ttacggtact catctacgtc gagggagcat tgttctcgta gttgggttat ggagtcgcgg
     3001 ctcggagact ttatagcgat cagtgcgcgt gaccatggct cccgtttcac ggaagcgaac
     3061 gggattccga ttaagtcgta cttaaagttt gtaacggaag ggttgaactg cctcaagcaa
     3121 aaaagctgct atcattaagg tccttatgga aactgatacg caacagatcg cgcttgtgga
     3181 tcatcataag ctcacccgct ttgtcgccac tgcttttgaa agactaggcg tgccatcacc
     3241 cgacgccgca atcgctgcgg atgttctggt gatggctgat ctgcgaggcg tggatacgca
     3301 cggtgtcatc cgctttaacc caagaagctg gtatgtgaaa tggctgcggg agggcgaaat
     3361 gagcgcgaca cccaagattc agatcgtatc ggaggccggc tcatccgctt tgatagatgg
     3421 tggccgtgga atcggcatgg tgattggcca tcgcgcgatg gaattggcca tcgaaaaagc
     3481 caaactttcg ggtgcgggca tggtaggagt ttgcaacagc cggcatttcg gcatgtccgc
     3541 atattatgcg atgcaagcct taccctatga catgattggc atcgcgatga cgaatgcagg
     3601 gcgtcaagtg gtgccgacgt tcggcagcga agcaagattt ggaacaaatc ccatttgttt
     3661 tgccgcgccg gcacaaaacg agcttccttt tgtcatcgac atggcaacga ccaccgcagc
     3721 tgcgggcaaa ctggagttag cggtgcgccg cggcgcatcg attcctctgg gatgggcgct
     3781 agacgagaaa gctgaaccta cgagcgatcc gaagattgca cagagggctc gtcgtctttt
     3841 acctttgggc ggcagccgcg aaggcggcag tcacaaaggg tacggcttag cgattttggt
     3901 tgagattctc tgcggggttt tgaccggcac ggtgaccgcc ctgaaccaaa atcaggaccc
     3961 acgaggacac tttttcgcag ccatcaagat tgattcattt cgtcctgtca gcgaattcaa
     4021 gcgcgatatg gaccgtctaa tccatcaatt gaaggcgaca caacccatag caggccaaga
     4081 ccgagtctac atcgccggcg aaatagaatt cgagacggca aagcgcagag cccaggaagg
     4141 tattcctttg cttggctcgg taatcaaggg tctgcgtgaa gtcggcgaac agctcgagat
     4201 tacctgtgat ctcgagtagc gctcttgcct gactcgctct tttacatcta gtaagaaccg
     4261 cggctgataa tgacttgctg aagcacaatc tccttcgtgg gtctatcgcg agaatcgcgt
     4321 ccaactttgg cgattgcatc gagcacgtct tgccccttga taacctcgcc gaagaccgtg
     4381 tgcttgccgt ccagccacgg tgtcgcttta tgcgtgatga agaattggct cccattagta
     4441 ttggggccgg cattggccat cgaaagaatt ccacttttgt tgtgcttaag ttccgggtgg
     4501 aactcgtcct caaatcgata accagggccg ccggtgccgt tacccaatgg atcgccgcct
     4561 tgaatcatga atcctgggat gacgcggtga aagatagttc cattatacag cggccgcttt
     4621 actttctcgc cggtcttggg atccgtccac tccttggttc cgcttgcgag accgataaaa
     4681 ttagccaccg tcttgggcgc tttatcttcg aagagctcaa ccacgatatc gcccatgctg
     4741 gtcttaaacg ttgcgtatac gggtattttc ttatccgtcg tctgcgctgc cattgtaatt
     4801 gaactccagg ttatgagcac cgcgacaatt cgaaaaattc tcgacatttt ttgcctcacg
     4861 ttgtgatttt taagcgttcg cgcccggcgc gtgcgccttg atgctagatt cactgtagaa
     4921 gtattgggtc agcaatctaa gcaattcatg gctcagcgtc aagcaattac gaggtaagtc
     4981 atgacgtgca attttcggcc tcgaatcgct tgaacggccc gccgttgggg ttattatcca
     5041 gatcgcaatt ctacgttcag tgttttgaca atgatatttc gggatggtat agcaaatttg
     5101 ctcttaacag ggactcatta ttatggcgtg gtttgatcga aaccccggtg gaaagaaaat
     5161 ttcagacgga gaggcggaga atgcttccat gccaacgcca tccactttag aacccgaccc
     5221 gccgccggtg gcggtcacgc ccaaaagcaa aatcgaaagt gccgttagcc cttccgaacc
     5281 gcgattggtc ggtcaccttc ataaaggaag tcggatcacg ggtcagcttt cttttgaagg
     5341 gtcggtgcgc atcgatggaa gcgttgaagg agagatccgc tgtctcgaga cattgacgat
     5401 cggcgaaggt gctgaagtgc gggcgagaat atcaagtcga gtcgtcatcg tccgtggaaa
     5461 agtagaaggc aatgtaacag ccaaagataa agtcgagttg ctggcgccgg gtcggctgtt
     5521 tggcaatgtc tcgacccccc gtctaatcat cacagagggt gtggtgttcg acggcgactg
     5581 ctctatggga gcggcaaaag aaggtggagt cgtgggttct agaaccttaa atgctgaaaa
     5641 agttgcggta gctcagtcat cgaaactaca gcccgattcc aataagtaaa cctcgctagt
     5701 agccgtttgt cccatccggt tttttacgtt cagacataga cggatcgtcg caaactctgg
     5761 ctggttcgac tcagcctggg catggactaa ccggaccggg tcgccgaggt agacgcgaga
     5821 tttagggatt gtcatgatcg cattaaatat ttctgtactt tggcaaattt tattgtttct
     5881 ggggctttgg ctgatcgtca gcaaggtcct gtttcggccc tatatgaccg tgttggaaca
     5941 gcgagagcag aaaactacgg gcgcagatga tagttctgag cacctggaac agcaggcaga
     6001 gcgcctgcgt gcgcagtacg aagaaaccat agtcaacgcg gctgccgcgg gaaacgcaag
     6061 caagaatgca attgttcaac aagcccgcca gcagagggag gccctcttga cttccgcgcg
     6121 ggaagaagcc gctggtattc tggagagagt acgccaagag gttcaaagtc agttagccca
     6181 agaacatgaa ttggcagttc gagaggctga tgcggttgcg caagatatgg tcagcaagat
     6241 cctcggcagg agagtgggat gaatccgcgc gcatggtttc tcgttgctga agcctgggct
     6301 gccggtggcg gtgagcatca ccccccggaa atatccggca tcatttttcc tcttctgaat
     6361 tttctgattt atgccgggat catttattat ttcgcattcc cactcattcg tagctttctg
     6421 aaagctaggc acgatgaggt tctggccaca gtcacggaag ccgcggcacg gaagcggagc
     6481 gccgaagctc tagtcgcaga ttatgaaagc cgtctagcta atatcgacca ggagattgac
     6541 tcgatccagg cctcgctgaa agccgaaggc gaacgagata aggcgcgcct acttagagaa
     6601 gccgaaggtc tcgctacaaa gattaaaagc gatgctcagc ttctcgccga ccaggaggtc
     6661 aaggctgcca agcaccaagt gcttctcgaa ttagccgaaa gagccaaagt cagcgccagc
     6721 gatctgatcc ggcgccattt gtcgcgagcg gaccaaggca gattagtcga agagtttatc
     6781 gatgacctcg ggcagattag atgatcgaag atagcttaag tcgcagatac tccaaggcgc
     6841 tctttgagct ggctcgagag tccgggcagg aagagagcat tgccgagcaa ctagagcggt
     6901 tttcatccac atccactgag tcgcgtctcg gtgctgtgtt gagcaatccg ggcttcgctc
     6961 tggacagtcg aaagaaaatc ctgctggaag tggctaagag cctacagctc tcttctataa
     7021 cagaacattt tctatcgctc ctgctcgacc gcgatcgcct ggcgtttatt ccttcgatta
     7081 tcgccagcta tcggcatctt ctgaatcagc ttaagggacg tgtcgatgcc aaggtaaccg
     7141 ccgccgaacc tctgggagcg atcgcgatcg agcgtctgcg tgactctttg cgccggcttt
     7201 ccggaaaaga tgtgatcctc caagaggaag ccaatcccga gctaatcggc ggcctagtag
     7261 tcgagtttca gggcaaaatt tacgacggaa gtgtgcgcac gcagctcgaa acgatgaagc
     7321 aacgcatcgc gcgcggtttt tgaaggagtt taaatggaaa tcggaacagc ggagatcagc
     7381 cgaattataa aagagcagat tcgtgactat gaaaaaacgg ttgaggtcca agagatcggc
     7441 accgtgcttt cgaccggcga tggtatcgcc cgcatttacg gactcgatcg cgtcgctgcc
     7501 ggtgagttac tcgagttccc gcataatata ttcggcttga cccttaacct ggaagaagac
     7561 aacgtcggcg ccgccttgtt cggcgagact cacatgatcc gagaaggtga tactgtcaaa
     7621 cgcaccggcc gtatcgccga agtaccggtg ggcaaagcgt tggtcgggcg ggtggtcaat
     7681 gcactgggcg aagcgattga cggtcgcggc ccgatcgaga ccagtgatcg tagacggatc
     7741 gagatcaaag ctcccgggat tgttgcacgc cagccggtga aggaaccgct gcagactggc
     7801 ttaaaggcta ttgacgcgat gatacccatc ggcaggggtc agcgcgagct gatcatcggt
     7861 gaccgccaaa ccggcaagac cgcggttgcc gtcgacacga tcattaatca aaagggcggc
     7921 aacgtcatct gtatctacgt tgccataggt cagaagcgct ccacggtggc tcaggtcgtg
     7981 gacaggctgc gcgaggcggg cgctatggac tacagcattg tggtcgccgc taccgcttca
     8041 gactcggcgc cgcttcagta cattgctccc tacagcggct gtactatagg cgaatatatt
     8101 cgcgataatg gcggccacgc gctagtgatt tacgacgatc tgtcgaaaca cgccgtggcc
     8161 tatcggcagc tctctttgct attgcgccgg ccgcccggac gtgaagccta tcccggcgac
     8221 gttttctatc tgcactctcg gttgttggaa cgtgccgcaa agatgagcga cgccatgggt
     8281 ggcggctcat taacggccct accgattatt gaaacccaag cgggcgacgt ttcggcatac
     8341 atcccaacca acgttatttc catcaccgac ggccagatct ttctggaaag cgatcttttt
     8401 tattcgggcg ttcgaccagc gatcaacgtg ggaatttccg tatcgcgggt cggtggtaa
//
LOCUS       DASUVS010000441         5929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1067859, whole genome shotgun sequence.
ACCESSION   DASUVS010000441 DASUVS010000000
VERSION     DASUVS010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067859"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1880)
                     /locus_tag="VF182_23205"
     CDS             complement(<1..1880)
                     /locus_tag="VF182_23205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEX6440045.1"
                     /translation="MKSSLKSLTSAVSGCVSRTFRERGERYYFDGAVNIIEGSAWRVA
                     ATVQGTRRYEVIIVRARDFFQVSCSCPLYDRELVTCKHLWAVFLAAEQNGFLDGLGDP
                     APNEIREEIPGLFNYHQSERGRTARSTKVSWKEQLQQLSGSMAAQDRRFNLDAAPERY
                     FVYAIEMEGCVDSERLTIQVAQRSRKADGSWGKLKSQKIRLNEIPALPDPADRRILAL
                     LAGAREESNYGYSPYYDSVQSTFSLAEPSWDLLLPLMCATERCILGFTADPDPQPLLW
                     DGGDLWEFGVEMTIDDSSEAYSVRGVLRRGEVELDLKRPQLIVAGGLLVHDGRIARLR
                     DFGAFSWMALLRDHGPMSVLKTEGDEFVNEILRMPHRPRLKLPQEFSFEEITCRPNPA
                     LTISSRSERKLVNTRLLGNLSFEYDGTLVPHRYPGQAIHQQSRRRLIKRDFAVEDAAV
                     NRLKELGFRRPHWPGSDSEFEFSPDRLPDVVRALSGEGWRVEAEGKVYRPASALRLSV
                     KSGVDWFELEGGAQFDQVKLALPKLLRAIKRGEETVRLDDGTLGILPEDWLEKYRLLA
                     GLGSMHGDQLRFSRKQVGLLDALIAGEEQVAVDAGFERVRQELYSFAGLKAADPTADF
                     VG"
     gene            complement(2392..3399)
                     /locus_tag="VF182_23210"
     CDS             complement(2392..3399)
                     /locus_tag="VF182_23210"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440046.1"
                     /translation="MNHSGLILPCAVFLTLVLSCAGAAQEKKLDKIRLGGGSASATQM
                     TMWLAKEGNYYEKHGLDAETISIPGSSLALQAMLSGELPIIQLGGAASIQANFSGADT
                     VIIATIVRKFLFSIFARPGIDKMEDLRGKVFGATRFGTLSDLASRFALRAYGIEPERD
                     ITMVQTGGPAETVTAMAAGKVHAAAISPPATLQARRVKLKELLDMSKLDAEYHINGVV
                     TTRRYLKTNEDVVRRFLRAYIEGAARAQKDKNFALKAMGKYFRTDDRELLEESYNAVV
                     KSNFAIPPYPSISGIASLLQGLEKQNPKAKGAKPEDFSDSRLIRELDQSGFVKSVLSE
                     R"
     gene            complement(3502..4275)
                     /locus_tag="VF182_23215"
     CDS             complement(3502..4275)
                     /locus_tag="VF182_23215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6440047.1"
                     /translation="MTNLTSSRLHTDRPPGHCRIGTSGFHYKHWKGIFYPENLSSKQW
                     FTYYASRFDTVELNNTFYRLPPESYFDAWRASAPKGFCFAVKFSRYGSHVMKLKNAED
                     TIPRFTERAERLADHLAPILVQLPPKWDVNVERLRDFLAAAPRRHRWTVEFRDPRWLI
                     KEVYDILRRYRAALCIHDMIPEHPREITADWVYIRFHGGRYDGNYDDAALAVYAREIG
                     SYLESGLDAYVYFNNDLHGHALKNAASLKALLRKHHRVS"
     gene            complement(4360..5880)
                     /locus_tag="VF182_23220"
     CDS             complement(4360..5880)
                     /locus_tag="VF182_23220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010174591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HEX6440048.1"
                     /translation="MATKPRPRNFTAGLLDGIERWGNRLPHPFTLFLLFSLVVLIFSS
                     LAHALGVAVLHPVTGKRIAAVNLLDRQGIQNIITKAVSNFTGFAPLGTVLVAMIGVGV
                     AERSGLFSAALKGFVRSVPSWLITGALVFAGVNASLTVDAGYVILIPLGAVLFAQVGR
                     HPIAGLAAAFAGVSGGFSANLFLTPLDPLLAGLTQEAARLYDPKYVVPATANYYLMIG
                     STFLLTIIGAIVTNWIIEPRLGSYTTQTEPGAPSLAIKEKLGLKAAALTFAIELILLL
                     FLVAPENALLRDAKGTLDPLFQGMVVLMALGFLLPGLAYGLAAKTITSNHDAARMIEE
                     TLGTMGGYIALAFAASQFVAYFGWSNLGLIMAVSGADLLRASGLSGIPLIILFVFVCA
                     AIDLFVASASAKWAVVGPVFVPMLMIVGFSPELTQGAYRIGDSFTNIITPLMPYMPLI
                     IAFAQKYQPSIGLGTLLTLMLPYSVSFALGWTLLLIIWLLLGIPLGPGSGVVYPPT"
BASE COUNT         1356 a         1688 c         1641 g         1244 t
ORIGIN      
        1 tgaccgacga aatcggcggt cgggtcggca gcctttagtc cggcgaatga atagagttct
       61 tgccgtacgc gttcgaaacc ggcatccaca gcgacctgct cttcaccggc gattagcgca
      121 tcgagcaaac cgacttgctt ccggctaaat ctcaattgat cgccatgcat gctgcccagg
      181 ccggccagca gccggtattt ctccagccaa tcttccggca atatgccaag ggtgccgtca
      241 tcgagccgga cggtctcttc gccacgcttt atcgcgcgca gcagtttcgg cagcgccagt
      301 ttgacttgat caaattgagc gccgccctcg agctcaaacc aatcaactcc tgatttgaca
      361 ctgagacgca gggcgctcgc tgggcgataa acttttccct cggcttccac tcgccaaccc
      421 tcgccgctta gcgcgcgcac cacatccgga aggcgatccg gcgagaattc aaattcgcta
      481 tcggatccgg gccaatgcgg ccggcggaag ccaagctctt tcaagcggtt gaccgccgca
      541 tcttccaccg caaaatcgcg cttgatcaag cggcgacgac tttgttgatg gatcgcctgt
      601 ccggggtatc ggtgcggcac cagggtaccg tcatattcga aagagagatt gccgagcaga
      661 cgagtattta ccaacttgcg ctcactccta gagcttatcg tcagagccgg attggggcgg
      721 caagtgattt cttcgaagct aaactcctgc ggcaatttaa gccgcggacg atgcggcatg
      781 cgcaggatct cattgacgaa ttcatcgcct tctgtcttga gcacagacat gggtccgtga
      841 tctcgcagaa gcgccatcca gctaaacgcg ccaaaatcac gcagacgagc gatgcggccg
      901 tcatgaacga gcagaccgcc ggcaacgatg agctgcggac gtttgaggtc cagttctact
      961 tcgccgcgcc ttaacacgcc gcgcacagaa tacgcttctg aggagtcatc gatggtcatt
     1021 tccacgccga actcccaaag atcgcctccg tcccacagca atggctgcgg atccggatca
     1081 gccgtgaaac cgagtatgca tcgctctgtg gcgcacatca gcggcaacag cagatcccat
     1141 gaaggttcgg ctagactgaa ggttgactga actgaatcgt agtacggaga ataaccgtaa
     1201 ttgctttctt cgcgcgcccc agccaatagc gccagaatcc gtctatcggc cggatcaggg
     1261 agagcaggga tctcgttgag gcggattttt tggctcttga gcttacccca actgccatcg
     1321 gctttccggc tgcgttgcgc gacctggatc gtcaaccgct cgctgtcgac gcaaccttcc
     1381 atctcgatgg catagacgaa atagcgctcc ggagcggcat ccagattgaa gcggcgatcc
     1441 tgtgccgcca tagacccgct gagctgctgg agttgctctt tccaacttac cttagttgaa
     1501 cgcgcagtcc ggccccgctc ggattgatga taattgaaca aacccggtat ttcttctctg
     1561 atttcattgg gagcgggatc acctaaaccg tcgagaaagc cgttttgctc ggctgccaaa
     1621 aataccgccc ataggtgttt gcaagtgacg agctcgcggt catagagagg gcagctgcac
     1681 gagacttgga aaaaatcccg cgcccggacg atgatgactt cgtatcgtcg cgttccttgg
     1741 accgtcgccg ccacgcgcca ggcgcttcct tcgatgatgt tgacggcgcc gtcgaagtaa
     1801 taccgttcgc cgcgctcgcg aaacgtccga ctgacacagc ccgataccgc tgatgttaag
     1861 gattttaagg agcttttcac aattgcttat cgacttacgg ccctgctaaa aagctaatca
     1921 cgcttggggc gccggcttgg tcgtgagaga gttgcttgac cgcagctgtt atattctctt
     1981 tggacaacga tcttctgctg taccggcagt acgaatgttt cttcgctaca ccgctcgaac
     2041 caattgtcga aacccgcgct gatattacga ggtcacgcgt acaggcaaac tgacagtcta
     2101 acagccggaa gcacgcaacc acaactcccc attatcagcg aatggccact gcaccggatc
     2161 gggcgagcaa tccttgggac cgccgcggca ccggattctt tattttcctt gatgccgcac
     2221 cagcatgcgc tgctggctca agcagctttg tcctgcgcag gacagctgac atctggcgat
     2281 gatgaactga acgaggattg cctatgagct tcggattgcc tcacaggcgc gcggcagggc
     2341 gtaaatgctg tagcgattac taggaagcgg cagctgctcg caagcggcgt gtcaacgctc
     2401 tgacagaacc gatttcacaa agccgctttg atcgagctct cggatgagac ggctatcgga
     2461 aaagtcttcc ggctttgcgc ccttggcctt gggattttgt ttctccagcc cctgaagcaa
     2521 actggcgatg ccgctgatcg atggatacgg cggaatcgcg aagttggatt tcaccacggc
     2581 gttataactc tcttcgagga gttcccggtc gtccgttctg aaatacttac ccattgcctt
     2641 gagagcaaag ttcttgtcct tctgcgctcg cgccgctcct tcgatgtatg cccgcaaaaa
     2701 ccgtctcacc acatcttcgt tggttttcaa ataacgccgt gtcgtcacca cgccgttgat
     2761 gtgatattcc gcatccagct tggacatatc caagagctct ttaagcttga ccctgcgtgc
     2821 ctgcaacgtc gcgggagggc tgattgccgc ggcatggacc tttcctgccg ccatcgccgt
     2881 caccgtttct gccggtcctc ctgtctggac catagtaatg tcgcgctcgg gttcgattcc
     2941 ataggcgcgc agggcaaatc tggaagcgag gtcggaaagt gttccgaagc gggttgcgcc
     3001 aaaaaccttg cccctgagat cctccatctt atcgataccc ggccgggcga agatcgaaaa
     3061 taaaaactta cgcacgatcg tggcgataat cacagtgtcg gcaccggaga agttcgcttg
     3121 gatggaggcg gcgccgccca gctggataat cggcaactca ccggagagca ttgcctgaag
     3181 agccagcgaa ctgccgggaa tgctgatggt ctcggcgtcc aagccgtgct tctcataata
     3241 gtttccttcc ttggcaagcc acatggtcat ctgcgtcgcg gacgccgagc ctccgcccaa
     3301 ccttatcttg tcgagcttct tttcctgcgc cgcgccggcg caggacagga ctaaggtgag
     3361 gaatacggca caaggcaaaa tcaggccact atggttcata ctggttctcc ttttagcagc
     3421 tccgtgtcgt gccaaaatag agagccaact gcctaggtgt caagcgatcg agccgggtcg
     3481 gtgatgctca gcaggacaag gctatgagac ccggtgatgt ttccgtaaga gggcttttaa
     3541 gcttgcggcg ttttttagcg catggccatg caaatcattg ttgaaataaa cgtaagcgtc
     3601 cagtccggat tctagatagc ttccgatttc tcgggcgtaa acagcaaggg ctgcatcatc
     3661 gtaattgccg tcgtagcggc ccccgtgaaa gcggatgtaa acccaatcgg cggtgatctc
     3721 tcggggatgc tcggggatca tgtcgtggat gcacaaggcg gcgcggtaac gacgtaggat
     3781 atcgtacact tcttttatca accagcgtgg atcgcgaaac tccacggtcc agcgatgtct
     3841 ccgcggcgcg gcggcaagaa agtctcgcaa gcgttctaca ttgacatccc atttgggcgg
     3901 cagctggacg agaatcggag ccaagtgatc tgcgaggcgc tcggcgcgct cggtgaagcg
     3961 cgggatggtg tcctcggcat tttttagttt catcacgtgt gagccgtagc ggctgaattt
     4021 aacggcaaaa caaaatccct tcggagcgct ggctctccag gcatcgaagt atgattccgg
     4081 cggcagccga taaaaggtgt tgttgagctc aactgtgtcg aagcgcgacg cataataagt
     4141 aaaccattgc ttggaagaaa gattctcagg gtagaagatt cctttccagt gtttgtaatg
     4201 aaacccggaa gtgcctatgc ggcagtgccc gggaggccta tcggtgtgta atcggcttga
     4261 ggtgaggttt gtcatctaga tcgatgcgcc agcgaccgcg actctccaat gagcgtaacc
     4321 ttcgcaactg gcgagtcaac agcgaacttc cgaggttgat cacgttggcg ggtaaacaac
     4381 gccggagccg ggaccgagcg gaattcctag aagcagccag ataatcagca agagcgtcca
     4441 gcccagcgcg aagctgaccg aataaggcag catcagtgtc agcagagttc caagcccgat
     4501 ggatggctgg tacttttggg caaacgcgat aatcaacggc atgtacggca tgagaggcgt
     4561 gatgatattg gtaaaagaat cgccgattcg ataagcccct tgggtcaact caggcgagaa
     4621 acccacaatc atcagcatcg gcacgaacac cggtccgaca accgcccact ttgccgatgc
     4681 gctggcaacg aacagatcga tagccgcgca gacaaagaca aacaaaatga tcagcggaat
     4741 cccggacagt ccggatgcgc gcaacaggtc agcgccgctg accgccatta tcaaaccgag
     4801 attggaccag ccgaagtagg cgacgaattg gcttgccgca aacgccagcg cgatatagcc
     4861 ccccatggtt ccaagcgtct cttcgatcat gcgggcggca tcgtggttgc tggtgatcgt
     4921 ctttgccgcc aatccgtagg ccagccccgg tagcaagaag ccgagcgcca tcaaaaccac
     4981 catgccctga aacaatggat cgagagtgcc cttggcatcg cgcagtagcg cattctcggg
     5041 cgctaccagg aacagcagta gaattagctc gatagcgaaa gtgagcgccg cagctttcaa
     5101 gccgagcttt tctttgatcg ccaggcttgg cgcgccgggc tccgtttgcg ttgtatacga
     5161 acccagtctt ggctcgataa tccagttggt gacgatcgct ccgatgatcg tcagcaagaa
     5221 agtggagcca atcatcaaat agtaatttgc cgtagccgga accacgtact ttggatcgta
     5281 aagcctggct gcctcctggg tgagaccggc caggagggga tcgagcggcg tgagaaacag
     5341 gttggcgctg aagcctccgg atacaccggc aaaggcggca gccaacccgg ctataggatg
     5401 gcggccgact tgggcgaaca gcaccgcgcc aagcggtatc aagatgacgt atcccgcatc
     5461 caccgtcaga ctggcgttga caccggcgaa caccaacgcg ccggtgatca gccacgacgg
     5521 cacacttctg acgaagcctt tgagggccgc ggaaaaaagc ccgctccgct ccgccacgcc
     5581 gacaccgatc atcgccacca gcaccgtgcc taatggagca aagccggtaa agttacttac
     5641 cgccttggta atgatgttct ggataccttg gcgatcaagc agattaacgg cggcgatccg
     5701 ttttcctgtc acgggatgga gcacggcgac tcccagagca tgggcgaggg atgaaaaaat
     5761 tagaactaca agggaaaaga gtagaaataa agtgaacgga tgcggcagcc ggttacccca
     5821 gcgctcgatc ccatcgagca aacctgcagt aaagtttctc ggacgcggtt tcgtcgccat
     5881 cgcccttgaa taatactgtc ggcgtaaatc agtcaagcag acgcctccg
//
LOCUS       DASUVS010000442         4364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1067897, whole genome shotgun sequence.
ACCESSION   DASUVS010000442 DASUVS010000000
VERSION     DASUVS010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067897"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(320..2014)
                     /locus_tag="VF182_23225"
     CDS             complement(320..2014)
                     /locus_tag="VF182_23225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009544259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX6440049.1"
                     /translation="MSFNSSTYVNFVSENGAAQERLFLQYLGYRSDFDIIIIGSGIGG
                     GVLADDLAERVGKQKRILVLEAGSFLYPTHVYNVCRFPNASLAKHFGCGTFWQSGNEH
                     SQNYIGEQPQLNFGGRSLFWSGLIPSIQGWELEFFPERVRQDLQSGLLARAGETMNES
                     RSMGATAQAVVSKLRQSSLAQDFSIQETPRALHQPYLTSEGVPSDQFFTEPTGVFNTA
                     ELLINQAGLTPGVNHGDGPGLQLLLNHFVEDVQNADGNRFELVTRNTLTGQIRNFNAG
                     TVVLAGGSIESPKLLRRSSLFPWLPDGVKNLVGRGLTDHPTSNEITTFVTNIGNVSIP
                     KHSHAKIIFYSRGLRDSQNRIRYPFNVEMNINHEYWHLRENDPNERDSGDSTPNSNSG
                     ASRMDIKFSFGNCLDNTNEVLPAPPFGYVPEVNFRNLKWMEYLAQSRFPAVAGWQKSY
                     DEIFAVLNQVTYQIFSQFQKDGQEARPENESWYGMDGKGFGWGTVHHAAGSLRMPHRT
                     RYDGPIESQSVVDVDLRVVGTQHLYVCDMSVMPFSSAANPVRTLVALALRLSNHFG"
     gene            complement(3244..3828)
                     /locus_tag="VF182_23230"
     CDS             complement(3244..3828)
                     /locus_tag="VF182_23230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440050.1"
                     /translation="MELQEFSTQHIKAVEFVIAATEDYAACRCCLLNGLFSGLYLGAE
                     AVEKYLKAFLLFVDPTIDVRKEYSHGITKLAAKACSLKPGFDLSQFTKVIDRLEMHYN
                     NRYPDHPKFKRDASTDELLAIDELVLLTVDSIPISEVPKFRNFGYFFIVCCPWEPRMN
                     PLKDWLERQNLALQRARDSLEQRYRAMEKELGCR"
BASE COUNT         1103 a         1161 c         1065 g         1035 t
ORIGIN      
        1 ccaatctcta aacaaacctt cagaagtcgt ggcttcattt tttcaccttt caacatagaa
       61 cgtatttaaa ctagaccttc agctagagct ttccaacaaa cgcaccccgc gcgtcaaaat
      121 ccaggagtga tttcaaaaga caagacttag agcttctgcc ttgtgatcgt accctggctc
      181 atcaggaacc cgttattctg cgatcctgcg catcaatatt aattgcgctg agtgtcatta
      241 gttagcgcag gcatcatccc tgataaatga gccctgctga agaaacgacc ccgaaccgac
      301 aacagcttcg tgagactcac tatccaaaat gattcgacaa gcgcagggcg agcgccacca
      361 gcgttcgcac gggattggct gccgaactga atggcatcac cgacatatcg cagacataaa
      421 gatgctgtgt gccgacaact cgcaaatcca cgtccacaac tgattgcgac tcgatgggtc
      481 cgtcatacct cgttcggtgc ggcatgcgca ggctaccggc cgcgtggtgc accgtacccc
      541 agccgaatcc cttgccgtcc atgccatacc agctctcgtt ttccggccgg gcttcctgac
      601 cgtctttctg aaattgcgaa aaaatctgat acgtcacctg attgaggacc gcaaatattt
      661 catcgtagct cttctgccaa ccggcaaccg ctggaaaacg cgattgcgcc aggtactcca
      721 tccatttcag gtttcggaaa tttacttcgg gcacataccc gaacggcgga gccggcagaa
      781 cttcgttggt gttatccaga cagttgccga agctgaactt gatatccatt ctcgacgcgc
      841 cgctgttact gttaggcgtt gagtcgcccg aatccctctc gttgggatca ttctcgcgga
      901 ggtgccagta ttcgtggttg atgttcatct cgacattgaa cggataccga atccggtttt
      961 gcgagtcgcg caacccgcgc gaatagaaaa tgattttcgc atgcgaatgc ttcgggatcg
     1021 acacattgcc gatgttggtg acgaacgtgg taatctcatt gctggtgggg tggtccgtga
     1081 ggccacgtcc aaccagattt ttcacgccat ccggcagcca ggggaaaagg ctcgagcggc
     1141 gcaacagctt tggactctcg atcgagccgc cggccagcac cacggttccg gcattgaaat
     1201 ttcgtatttg gccggtcagg gtattccgcg ttacaagctc gaagcgattg ccatcggcat
     1261 tctgcacatc ctcgacgaaa tgattcagta gaagctggag gcccggacca tcgccgtgat
     1321 tgacacccgg cgtgagcccc gcttgattga tgagcagctc ggcggtgttg aacacgccgg
     1381 tcggttcggt aaaaaattga tcgctcggga cgccttccga cgtcaggtac ggctgatgca
     1441 gagcgcgggg cgtttcctga atcgagaagt cctgcgctaa cgagctctgc cgcagcttgc
     1501 tgacgactgc ctgcgctgtg gcacccatcg atcgcgactc gttcatcgtc tcgcccgctc
     1561 tcgcgagcag accgctctgc agatcctgcc ttacgcgctc agggaagaac tcgagctccc
     1621 atccctgaat cgacgggatc aagccggacc agaaaagcga acggccgcca aaattgagtt
     1681 gcggttgctc gccgatgtag ttctgtgaat gttcgtttcc ggactgccag aaggtgccgc
     1741 agccgaagtg cttggctaga ctcgcgttgg gaaaacgaca gacattgtaa acgtgggttg
     1801 gatacaggaa tgatcccgct tccaaaacca ggattcgttt ctgcttaccc actcgttctg
     1861 cgagatcatc cgccagcacg ccgccgccga ttcccgagcc gatgataatg atgtcgaagt
     1921 cggatcggta tcccaggtat tgcagaaaga gcctctcctg tgcggcacca ttttcagaaa
     1981 cgaaatttac gtaggtcgat gaattgaagc tcatcgcaac tcctcaaaga aataatcgga
     2041 ctacacttca cccgaaccga tcttaatcga agttttgcta accgaaaaga gtgtgaacct
     2101 ttcaaaagaa caaatcgaca tgaaaatttt ttttactaga attagcatcc ttgtctgcag
     2161 ctgaagcgaa attattgatt ctagtgaacg caaactggcc cgacagatca tcgccgagaa
     2221 cttcctcggc cgtcagcgcc cgtagcgcca gcacagagga aatcactagt tgaggagctc
     2281 gcccgcgcca tggagatggt tgagacgcag aaccacaacc cgaccactat gggagtccgc
     2341 tacaacaatt tgaaatgtga agtgagggag gaattatggc tcagtagaaa tgccgaatgc
     2401 ttcgccacat tcgcggcgac cctgaacgcg ctcggtcaac taactcgcaa ccgtttgctg
     2461 gccctgttct caacccagtt ttttgccgct tccagtccgg cgttttctgc gtcctcatag
     2521 cgtgagaatg gttcaaggca atcctggatc gaatgcagct ggcgcgggag ggctttgctc
     2581 tgcgcccagc taatcgaagc aacgcatttc cactttcccg tctcgtcatc gtacatgggc
     2641 gtactgacga ttgtatggtg gtgataaatc aggagagcca tggaaaggtc cccctaagtc
     2701 ccggccgtag ctaggccggt cggtcaaaat gcaaccgaaa agcggagcga aaattatgct
     2761 cagatggtag cctaaaggag cttaaaagaa aatagaagga atgttaacgc agatcgaata
     2821 atgcgtctta accgagccgc ctccgcttgc tggtcttttt ctttttttct tgagctgcgg
     2881 cgtttctatc gccgatctca cggcgaagct tctcaagctc taatttgttt ttctccagtt
     2941 caacactggc gtcgacactc tcgcgacgtt ctttccgcca tgtcataaca atcgtaacca
     3001 ggaatccaaa aaacgccgta atacatatta ctaatgacgc gaaaaggatg aattcttcgc
     3061 ttcgtagtag gccaggccag gcgttcgtcc atgccgcgaa tcctaataag aacgtcacta
     3121 gcagtgacgc gacagaaatc cacccgagcc aacgacggat cgtttttatt tgttcaaaag
     3181 gaagaggcat cgctttttac tcccgattcc gtttcgcaag atgcaggttg tgcgacgcta
     3241 cagttaacgg cacccaagct ctttttccat agctcggtat cgttgttcca aagaatctcg
     3301 cgcgcgctgc aatgcaagat tttgtcgctc caaccagtcc ttaagtggat tcattcgcgg
     3361 ctcccaagga cagcatacaa tgaagaaata cccgaaattg cggaatttcg ggacttctga
     3421 aatcggaata gaatctacgg taagcagtac gagttcgtct attgcaagca attcatcagt
     3481 gctcgcgtcg cgcttaaact taggatggtc cggatatctg ttattgtaat gcatctcaag
     3541 tcgatcaatc acttttgtga actgggagag atcaaagcca ggttttagag aacaagcttt
     3601 agcagccaac ttggtaatgc cgtggctata ctcttttcgt acatcgatcg ttggatcgac
     3661 gaataacagg aatgctttta agtacttctc tacagcttca gccccaagat aaagaccaga
     3721 aaatagaccg tttagcaaac aacagcgaca tgccgcataa tcctctgtcg ccgcaattac
     3781 gaactcgacg gccttaatat gttgtgtgct aaattcttga agttccacaa aattcatccc
     3841 ttccttttag cacaaggctc gtccacttcc cagattggct gactgattct cgagtgcagg
     3901 atggaacaga aaccaagtat cggacgcata gtggtctata acaatcctgc actgaggacg
     3961 ggacgtcccc gccaacacag tccccgacat ggagaaaatc gccaaatgcg aatctggcgg
     4021 aactcactac aggcaagacg gaaggctcgt taagaacgtc aataagaatg ggaccatgga
     4081 gttcggaaac ttccagatca atcagctggc gaagcgaaaa gtcgcttcca ttgtctacaa
     4141 catcaatctt ccggaagaaa acgatgtctt tgcgaactgg ctctatgaca catctggtac
     4201 aggagattgt cacagctaga aacatgttgg tctatataat gcaattcaca accgagtcgc
     4261 gcatcgacgc caggctgttt gtcgttgaca ttttgagagc tcatcgacta ttgaggatct
     4321 cacggaggaa agcgttcgga tcgccctttg tacgttaagt cgcg
//
LOCUS       DASUVS010000443         3186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1290003, whole genome shotgun sequence.
ACCESSION   DASUVS010000443 DASUVS010000000
VERSION     DASUVS010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290003"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VF182_23235"
     CDS             <1..909
                     /locus_tag="VF182_23235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440051.1"
                     /translation="KRFGFVPRVHLTPVTNPDSLLRFLTEAKVGRIDADLLYAGAADI
                     AGRPDRLALFEDIEAPLVKIFSPKFKGLEGVLRNVAPWKRPFSVDVTTLASGIIYNTK
                     KINANDLPKTYEEFAEGKGFADPKWKGNFAINTIGPASPLTDMGVGGHWDIEKQKKVL
                     QMLLANKPLIKRSSGDVRTAVALGEVAAATGNIGGTEGLRKEGYPIQSRLFEDVMAIG
                     SIGLTIPKGAKSPNLALLYLAFILEDGLPIVERVNGEGTFVDPRSQLAKSLKALPKAK
                     ILEWSPEEVLAGQRDKIRKALQPLMP"
     gene            924..2024
                     /locus_tag="VF182_23240"
     CDS             924..2024
                     /locus_tag="VF182_23240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6440052.1"
                     /translation="MLKVEGLTRTFVTGDGQVQAVHDVSFAVEKGEFFSLLGPSGCGK
                     TTTLRCIAGLETPDSGEIWIDDKPVFSSKKGIVVPVHEREIGMVFQSYAVWPHMTVGQ
                     NVGYPLVHGRRKLPRTETKKKVKEILHLVQLDDLEARPSSLLSGGQQQRVAVARALVA
                     EPQLLLMDEPLSNLDAKLRVEMRHELRDLFERLKLTALYVTHDQEEALVLSHRMAVMH
                     GGRVVQVGPPMDIYKEPVNLFVAGFVGTTNLLEGRVVDNETAGARGLVETAVGKLRCA
                     LPQQVVQGQRVSLMFRPEAVSIHTAACRSTVNVLPGQVRAVVFTGNRIEYDIDVGEYH
                     VRADTNPYVAPLERGQSVWLEMPADRIRVLAA"
     gene            2065..2832
                     /locus_tag="VF182_23245"
     CDS             2065..2832
                     /locus_tag="VF182_23245"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX6440053.1"
                     /translation="MNFVDLTMPLNHRWMPDEGLPTAIKFFLGPKNHQEKGMVVGSDS
                     GTSLALPALFADFRKTTRLDQVPVEKLFLRPAAIATINKGAGEEISKSEAQQALGAAR
                     PAKGDALLIVTGWGDKPRHQAAGNEYVLSSPHFSREAAQYLAQAMRDNDNDLLLVDTA
                     VLGLPQSYIIPEWFSMLPSPAAESAEARMYLHLYDGEKAKKDFAVDMEFARMGIMTVR
                     KLIGCGQIKSRRIKMIVSPLNVVRGVGSTCRVVAVEE"
     gene            2835..>3186
                     /locus_tag="VF182_23250"
     CDS             2835..>3186
                     /locus_tag="VF182_23250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440054.1"
                     /translation="MKMIDLTMPIWEGAGYGEILPFTNSSVRLWEYMYYDKHGLRMTR
                     MKLDGETGSPFMVPHQRMPFDPTPLQPDPKFSWTLDQIPLDRLILRETVILDIRAPEQ
                     HEITVEEMDKAMKGA"
BASE COUNT          717 a          815 c         1004 g          650 t
ORIGIN      
        1 aaacgtttcg ggtttgtgcc gcgggttcat cttacgccgg tcaccaatcc ggattctttg
       61 ctcaggttct tgaccgaagc gaaagtcggc cgcatcgatg ccgatttgct gtatgccggc
      121 gcggcggata tcgccggccg acccgatcgg ctggcgctgt tcgaggatat cgaggcgccg
      181 ctggttaaaa ttttcagccc gaaattcaaa ggcctcgaag gggttcttcg caatgtggcc
      241 ccatggaagc ggccgttttc ggttgacgtg accacgttgg cctcgggcat catttacaac
      301 actaagaaga tcaatgccaa cgacctgccc aagacctatg aggagtttgc cgaaggcaaa
      361 ggttttgccg atccgaaatg gaaaggcaat tttgccatta acacgatcgg tccagccagc
      421 ccgcttacgg atatgggcgt cggcggtcac tgggacatag aaaagcaaaa gaaggtgctg
      481 caaatgctgc tcgccaacaa gccgctgatc aagagaagca gcggtgatgt gcggacggcc
      541 gtggcgttag gcgaagtcgc tgcggctact ggaaatattg gcggaaccga aggtctgcgc
      601 aaggagggct acccgataca atccaggctc ttcgaagacg tgatggcgat cggctcgatc
      661 ggccttacca ttcccaaagg cgccaagagc cccaatctgg cgctactcta tctggccttc
      721 attctcgagg atggcttgcc catcgtcgaa cgggtcaatg gcgagggcac gtttgtcgat
      781 cccagatcgc agctcgccaa atcactgaag gccctgccca aagccaaaat attggaatgg
      841 agtcccgaag aggtgctcgc cggccaacgc gataagatca gaaaggcact gcaaccgttg
      901 atgccctagg gaagtcgtcg tcgatgttaa aggtagaagg actgacacga actttcgtta
      961 ctggtgatgg ccaggtgcag gccgtgcatg atgtcagttt cgccgtcgaa aaaggggaat
     1021 tcttttcgct gctgggaccc tccggctgcg gtaagacaac cactcttagg tgtatcgccg
     1081 gactggagac gccggattcc ggagaaatct ggatcgacga taagccggta ttttccagta
     1141 agaagggcat cgtggtgccg gtgcacgagc gcgaaatcgg catggtgttt cagtcctatg
     1201 cggtgtggcc gcatatgacg gttgggcaga atgtcggcta tccactggtt cacggccggc
     1261 gcaagctgcc gaggactgag acaaaaaaga aggtcaagga gattctgcat ctggttcaac
     1321 tcgatgatct ggaagcgcgg ccttcctcgt tgctgagcgg gggacagcag cagcgtgtcg
     1381 cggtagcgcg cgccttggtg gcggagccgc aacttcttct catggatgag ccgttgagta
     1441 atctggacgc caagcttcgg gtggagatgc gtcacgagct ccgcgatcta ttcgaaagat
     1501 tgaagctgac cgctttgtac gtgactcatg atcaggagga agctctggtg ttgtcgcacc
     1561 gcatggcggt gatgcacggt ggccgggttg tacaagtcgg tccgcccatg gacatctaca
     1621 aggagccggt caatctattc gtcgccggct tcgtcggcac gaccaacctg ctagagggcc
     1681 gggtcgtaga taacgagact gccggcgcgc gggggctcgt agaaaccgcg gtgggaaagc
     1741 tgcgctgcgc attgccgcaa caggtcgtcc aagggcaaag ggtctcgctg atgtttcgtc
     1801 ccgaggcggt atcgatccat acagcggcgt gcaggtcgac agtcaatgtg ttgccgggtc
     1861 aagtgcgcgc ggttgtgttt accggtaatc gtatcgagta cgacatcgac gtcggagagt
     1921 atcacgtgcg cgccgacacc aacccatacg ttgcgccgct ggaaagagga caaagcgttt
     1981 ggcttgagat gccggccgat agaatcagag tgttggcggc gtgacggatt tgccggagca
     2041 gcgccgaaga aaggtggagg aaatatgaat tttgtcgatt tgacaatgcc gttgaaccat
     2101 cgatggatgc ccgatgaggg tctgccgacg gcgatcaaat tttttttggg gcccaagaac
     2161 catcaagaaa aaggcatggt tgtggggagc gattcgggaa caagcctggc gttgccggcg
     2221 ctgttcgccg atttccgcaa gacgacccgg ctcgatcagg tgccggtcga aaagttgttc
     2281 ttgcggccgg cggcgattgc caccatcaac aagggcgctg gagaggaaat aagcaagtct
     2341 gaggcgcagc aggcgttggg tgccgcccgt ccggctaaag gggatgcgct tctgatcgtc
     2401 accggttggg gcgacaagcc gcgccatcag gcggcgggaa acgaatacgt cctgagcagc
     2461 ccgcatttct ctcgcgaagc ggcgcaatat ctggcgcaag cgatgcgcga caacgacaat
     2521 gacttgctgt tagtcgatac ggcagtgctc ggactgccgc aaagctatat catccccgaa
     2581 tggttctcga tgctgccgtc gccggccgcc gagtcggccg aggcgcgcat gtatttgcat
     2641 ctctatgacg gcgagaaggc caaaaaggat ttcgccgtag acatggaatt tgcccgaatg
     2701 ggcatcatga ccgtgcgcaa gctgatcggc tgtggccaga tcaaaagccg gcggatcaaa
     2761 atgattgtgt cgccattgaa cgttgtccgg ggcgtaggct cgacgtgccg agtcgtagcg
     2821 gtggaggaat gaccatgaaa atgatcgatt taaccatgcc gatttgggaa ggcgcgggat
     2881 acggagaaat tttgccgttt acgaattcct ccgtgaggtt gtgggagtac atgtattacg
     2941 acaagcacgg cctgaggatg acgcgaatga aactcgatgg cgaaaccggc agtccgttca
     3001 tggtgccgca tcagcgtatg ccgtttgatc cgacaccttt gcagccggac ccgaagtttt
     3061 catggacctt ggaccagatc ccgctcgatc gactaatcct tagagagacg gtgatcctgg
     3121 acatcagggc tccggaacag cacgagatca ccgtcgagga gatggacaag gcgatgaaag
     3181 gcgccg
//
LOCUS       DASUVS010000444        12567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_195759, whole genome shotgun sequence.
ACCESSION   DASUVS010000444 DASUVS010000000
VERSION     DASUVS010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12567
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195759"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            531..1017
                     /locus_tag="VF182_23255"
                     /pseudo
     CDS             531..1017
                     /locus_tag="VF182_23255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006621876.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methanogen output domain 1-containing protein"
     gene            2212..2787
                     /locus_tag="VF182_23260"
     CDS             2212..2787
                     /locus_tag="VF182_23260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440055.1"
                     /translation="MENQQSETVAGVSDGGAINKVKSEAAAVAQDVRRQGQEQFEARK
                     QTAADQTEKLAGAVERISEELKDQDQESLADYAGQLAGGMRNFADSLRQRNLDELVRD
                     TQQLARDNPTLFLMGSVAVGIALSRFLKASSQRSGASRHDESEFYGSSQSVVQPVTFE
                     SRPSVIRESATTTALSTADSPSFADDVDKGV"
     gene            2790..3227
                     /locus_tag="VF182_23265"
     CDS             2790..3227
                     /locus_tag="VF182_23265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX6440056.1"
                     /translation="MAKQPPFQATPAIESSRVDREPDSAAGLLSRLMGEVSTLFRKEI
                     ALAKAEVSEAASEAKAGAISLVAGGAIVFAAVLVLLGAIVLLLAERMEPWLAALIVAL
                     VVGVIGFVMIQSGKKKLDPLSFKPERTQDALRKDKEMVQRRAS"
     gene            3224..4054
                     /locus_tag="VF182_23270"
     CDS             3224..4054
                     /locus_tag="VF182_23270"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX6440057.1"
                     /translation="MSDLNNRSNVRDDARKDPATLEREIDQTRANMDRTLGALERKFS
                     PGQLLDQAMEFARENGGEFANNLGRSVKENPVPMLLTAVGIAWMAASSNRAKSPTADT
                     YDDRYARNDLSAVDFDDTGYEDDAGDEKEGLSDKAQRLKASAEGTLTEAGQRVKSAAE
                     RARQKLTGTKDTVSAGLRRTSGTAQVQTQRVRESFNSLLTEQPLLLGALGIAVGAAIG
                     AALPGTEKEDRLFGSASDKALAEVKQRGSETYEQVRDKVNAVGEEAKQSISNTVREST
                     "
     gene            4193..5095
                     /locus_tag="VF182_23275"
     CDS             4193..5095
                     /locus_tag="VF182_23275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX6440058.1"
                     /translation="MQRKSIWQFLKTTINEWTEAEPFQLSAALSYYTLFSLAPLLLIA
                     IGVAGLAFGREAAQNQIVETLQGMIGQDSAKAVQDMIQASNEKPKTGIVSTVIGFVVL
                     LFGAGGVVGQLQTSLNKIWEVTPKPGQGIWGFLRQRFFSFAMVLAIGFLLLVSLVVTA
                     VLSSFTAMLNSYLGNATFVAHAIDILVSFGFVMLLFALIYKYVPDVDIQWRDVWIGAA
                     ITSILFTVGKFLIGLYIGTSGVSSTFGAAGSLITVLVWVYYSSLIFFLGAEFTRVYAT
                     QYGSGVVPAENAQPMDAAPQSANA"
     gene            complement(5242..5418)
                     /locus_tag="VF182_23280"
                     /pseudo
     CDS             complement(5242..5418)
                     /locus_tag="VF182_23280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010794153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            complement(5451..5573)
                     /locus_tag="VF182_23285"
     CDS             complement(5451..5573)
                     /locus_tag="VF182_23285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440059.1"
                     /translation="MDGHRELENPLSLPQWLAGWAIILGTCVVFWLTVYRWALN"
     gene            complement(6936..7748)
                     /locus_tag="VF182_23290"
     CDS             complement(6936..7748)
                     /locus_tag="VF182_23290"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEX6440060.1"
                     /translation="MNRREFIEHGAITAGATVNSGCSLRVPPITDQTSHRPWPLPKSR
                     WMMFMRWHDLLFLHWPIRPELIRPLIPTILELDTFDRWSWVGIIPFHMSGVRPRYVPL
                     PLTLPELNVRTYVKSAGKSGVWFFSLDAASWIAVRAARWLGLPYFDARMKVESATDTI
                     RYDSVRIHKNSPRAEFRAEYGPSGAVFRSTAGTLDHWLTERYCLFAGLKPDHVVFGEI
                     HHPPWALQPAEVKLDVNTMTHSIGIDLPEQKPLAHFARFQEVVAWPVVPLET"
     gene            complement(7801..8559)
                     /locus_tag="VF182_23295"
     CDS             complement(7801..8559)
                     /locus_tag="VF182_23295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6440061.1"
                     /translation="MTIHDEPIEIAVDDQKIEGRLITGVSAGPAVLFVHGWGGNQRQY
                     LNRAQAVAELGFICLTFDLRGHARSDGQRELVSREDNLRDLLAAYDALASQPGADTSA
                     LGVVGSSYGAYLAAIMTRQRSVRWLALRAPALYNDAGWELPKRKLHEDPDFCAYRRRA
                     VAPEENRALRACAAFAGNVLIVESEQDDVVPHPVIANYLAAFARARSVTHRLIKDADH
                     GLSDAAWQRTSTSFLLQWIAQRIIGTSVQAEARL"
     gene            complement(8543..9691)
                     /locus_tag="VF182_23300"
     CDS             complement(8543..9691)
                     /locus_tag="VF182_23300"
                     /inference="COORDINATES: protein motif:HMM:NF022821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3182 family protein"
                     /protein_id="HEX6440062.1"
                     /translation="MIVIRNRVLNRALWALGAVVQYTDPSSHAPARHESVTRMLLARK
                     LAALKGYEFAGEYNPDTHYAGPLYFVPNDTLTKSELARKVDIRDHHDLFGGVVPYPFA
                     ATKTITHPLVSPHAFAPAGWSHEFARSVRDVVLFGFSAFAPDDLRRAAQRVLARGHAR
                     LKPARGIGGLGQTVIKSSDELDALLDTIDPAELAQYGAVIEQNFDHIETYSVGQTFVA
                     DLSATYYGRQLLTRDNRNENVYGGSDLTVVRGDYDDLLSLELPPQARSAINNARTYEA
                     AAINNFPGWLCSRRNYDVAQVVDDEGRQRCGVLEQSWRIGGASAAEIAALEAFRADPG
                     LRSVRAASIELYGDGQTPLDAVVHFRGVDDRVGPITKYSQVEAYDNPR"
     gene            10277..11389
                     /locus_tag="VF182_23305"
     CDS             10277..11389
                     /locus_tag="VF182_23305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6440063.1"
                     /translation="MSRILITGGAGFIGSHLADELLHSGHAVKIFDLLCSQVHGTENQ
                     PPEYLNPDVELVLGDVRDRAAVDRALKGIDAVYHFAAAVGVGQSMYEVEHYASINDIG
                     TAVLLEALIKRPVERLIVASSMSVYGEGLYRSRRGAIHCAVERSPTQLRSARWDPVDD
                     DGVALEPIPTPESKTPALSSVYALSKYVQERMCLMIGRAYNIPTVALRFFNVYGPRQA
                     LSNPYTGVLAIFASRLLNGKAPLIFEDGEQQRDFVNVRDVVQACRLSLDAASAPDRVF
                     NIGSGQAFTITELAERMADALGKNVEPQITGKYRVGDIRHCFADISLARSMLDYAPQI
                     SLEDGVLELADWLEGQIADDHVDSASAELAARGLTL"
     gene            11386..>12567
                     /locus_tag="VF182_23310"
     CDS             11386..>12567
                     /locus_tag="VF182_23310"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6440064.1"
                     /translation="MKKARSGRNGAAGIKPDVCKHPSLRAQIAEADSGAGVRIGVVES
                     FRIGDRAGVENVLADLRELRVADLRTTISWADWDSPEGEAWYSWLLPHLARQLNVIPC
                     VLYTPLSQAVAPRVSAPPWDPKAYGDFIDVLITRLGSCFEWIELWNEPDRLSEWDRTL
                     DPHWQTFCAMIGGAAYWAQRRGKRTVLAGLNAFDPNWLELMFRRGVMQYIDAVGVHGF
                     PGTSEANWESWPAVIQSVQQILTQHRSGAEIWISATGYSTWRHDEYEQLRAFARALEA
                     PVERLYWHAARDLDPEFSTRDGFHADDRDYHFGLRRSDGSEKLLARLWGEGGLDAVWG
                     ALEPHPGARGSAAKPTQPVVITGGAGFIGTNLADRLANEGERVLVFDNLSRPGVERNM
                     KW"
BASE COUNT         2827 a         3369 c         3575 g         2796 t
ORIGIN      
        1 tgatgccatc gccgagcgga ttgcgagtat tttggataac tgcggcgtgg tcgacatcga
       61 ggaagaggct gaccgggatc ttaaggaagg gcgcgctgtg ttcggaagcg agacggattc
      121 gtcgcggctg aaatgagcgg gggtagagcc ttcaggaatg atgaacttga tcagcccggt
      181 tccccgcctg ctgctcgacc tgccgattta gatcgagaca tcgagttccg cgcattctga
      241 cgtcagagct gacacgagta gaagatcgga tgaccttggc caagcggact ctgccacgat
      301 tcccgtcaag gaagaacaat tgattgtccg caaacacaag gtcgaaagcg cgcgggtgcg
      361 tatttacggg cgcgtggtcg agaaaccggt ggagcagtct gttcaatgca tgatgagaaa
      421 attgtcgtcg agctcgacga gctgctggat catgtgcgac agctcacatc tccctgatgg
      481 aaggagatga tgagactcga tgaaatgtac attccttaga acctgatctc ttgttgcgtt
      541 ctctaatccg ggagctgtcc gggcgttctt gaagatgtcg tcggcataaa agaaagcttc
      601 gggatttgtc agcgtcgtcg gaagatccat tggcgaatgg atgaacgggg aataccgcaa
      661 ggctctgcgc atttcgaatc tcagccgcga acaagtgccc caagtttgtg tcgatctcaa
      721 aaggcgtagt taagtaagtt tcacatcatc gaggaagacg aagataaaat catcttcggc
      781 aacacggcct gcccgtttgg cgataaggta ttggccggga atccatgtgc atgatgactt
      841 ccaatgtatt ttgccatatc gccgcggaaa acgttcgcta taccaaagtc gagtttcaat
      901 aaacgattgc ctgtggcaac aaaggatgct cgtgtcgtcg tctatcttca gcgggcctcc
      961 gcttcgagtg agggcaggga gtatttccgc atctcttcaa cgacacgctg gttttagaaa
     1021 taagcgatac cggcaaggga attcccgaag gcattgatgt tttcgaaata ctccgaacca
     1081 ccaacccgac ggtcggggtt tgggtctgcc tctggtcagc aagataattt ccgctccccg
     1141 cggcacggtt gattactgcg gccagccggg aaaatgtaca acttttagtc tgtcgttacc
     1201 tgtcagataa ctccgagcta ttagggtatc ccctattcag ccattggtga aaggtcgctc
     1261 gatctggccg atcaactcac tgccctgata ctcgccgtcc ttgagatctc cgtcttcctt
     1321 ctgcttttgt gatcctcgcc cgagctgatc cggcagcagc ggtaccgtaa gttttcatct
     1381 aatccaaatc tttcctcacg aaggttctat cacggccagc cggctctttt cgagtgaact
     1441 ctggatagag ctcaggtgcc ggcggacgtg gctcaaccat cgcgaccgat ttttggttac
     1501 ctttggggcg atcgatgaac gctcgactat gctctgccgg cgcagattgc agaagaaatg
     1561 ccgatgcctc catggggatg gtcgatccgt tcgcggtggt cacatgctca acaatttagt
     1621 tcgctagaac tgcgacataa tcagcgggaa atttccggca gcggatcgga taatgcggag
     1681 gtgtattccc gtgagccgat ggagttgtta agctgatctc atgacaaaaa cacgtaaagc
     1741 gctgcgcttc gatgcttact cggcacctac agagataacc caagtggatg gcagggctct
     1801 ctccccagaa gaagttaatc ttgcaaagct gataatcgat aagctcgcgc gcactgcaag
     1861 ggcaagcgtg atcgatttta gtcgggcttc gaaacgacgc ctgagacgat caaggtcacg
     1921 gaatcctggc cggtgagttt caccgcccgg caacggattt agcctcatag atttgacagc
     1981 ctcttcagcc gtcttccgca gagttgccac cctaagcact taggtttcgt ccccttgatg
     2041 cgcctttcat tcatcaatgt ttcgcagtca tgcgacattg atccacctgc tgcgagccaa
     2101 atgcccgagc tatgctagtg gcatagccat tgcgtttcct tcctgcattc ccaccgcgtt
     2161 aatccgagag tcagaactta gaacgactat ttcaagaagg agtgtttatt catggagaac
     2221 caacaaagcg aaacggtcgc tggcgtaagc gacggcggcg caatcaataa agtgaagagt
     2281 gaagccgcgg cagtggcgca ggatgtgagg cggcaggggc aggaacaatt cgaagcacgt
     2341 aaacagacgg cggcggatca aactgaaaag ctggctggcg cggtcgagcg catctcggaa
     2401 gaactcaagg atcaggatca agaatcgttg gctgattatg cgggtcagtt ggccggcggc
     2461 atgaggaatt tcgctgacag cttgaggcag cggaacttgg acgagctggt cagagatacc
     2521 caacaactgg cgcgcgataa tccgacgctt ttcttgatgg ggagtgtggc cgttggaatc
     2581 gcgctctcga ggtttctaaa agcatcgagc cagcgtagcg gcgccagccg acacgacgaa
     2641 tctgagttct acggctcgag ccaatcggtc gtgcaaccgg tcacttttga gtcgcggccg
     2701 tcggttataa gagagtcagc cactacaaca gcattgtcaa cggcggacag cccgtctttc
     2761 gctgacgatg tagacaaagg agtttgacca tggcaaaaca accgccattt caagctacgc
     2821 cggcaatcga gtcatctcgc gttgaccgcg aacccgattc ggcagccggc ctgctaagtc
     2881 gtctcatggg ggaagtctcg acgctgtttc gcaaagagat agcgctggca aaggcagaag
     2941 tatccgaagc cgcttcagaa gcaaaggccg gagccatcag cctggtagcc ggcggcgcaa
     3001 ttgtatttgc cgctgttctc gtcctgcttg gcgcgattgt actgctgcta gccgagagaa
     3061 tggagccttg gttggcggct cttatcgtcg ccctggtggt aggggtcatt ggctttgtga
     3121 tgatccaaag cggcaagaag aagttggatc cattgtcttt caagcctgag cggactcagg
     3181 atgcgctgcg caaagacaag gagatggttc aaaggagggc gtcgtgagcg acctaaataa
     3241 cagaagcaac gtcagagatg atgcgcgaaa agatccggca accctggaac gggagatcga
     3301 tcagacccgg gcaaacatgg accggacgct cggcgcattg gaacgcaagt tttcaccggg
     3361 acaattgctg gaccaggcga tggagttcgc tcgagagaac ggcggagagt tcgcaaacaa
     3421 tctcggccgt tcggttaaag aaaatccggt gccgatgctg ttaaccgcgg tgggaatcgc
     3481 ctggatggcc gcttcgagca atcgagccaa atcgccaacg gccgatactt acgacgaccg
     3541 ctatgcacgg aatgatttaa gcgccgtcga tttcgatgat acaggctacg aggatgatgc
     3601 aggcgacgag aaggaaggct tgagcgataa ggcccaacgc ctcaaagcct ctgccgaggg
     3661 aaccttgact gaagccggtc agcgggtgaa gtccgccgcg gagcgagctc gacagaagct
     3721 cacaggcact aaagatacag tgtcagcggg actgagaaga acttcaggca cggcccaagt
     3781 ccaaacgcaa cgagtacgcg aaagcttcaa cagtcttttg actgaacagc cactgctcct
     3841 gggcgctttg gggatcgccg taggagctgc cattggcgcg gctctgccgg ggacagagaa
     3901 ggaagatcgg ctgttcggtt ccgcgagcga caaagccctc gctgaggtca agcagcgcgg
     3961 gagcgagacg tatgagcagg tgcgcgacaa ggtgaacgct gttggcgaag aggcaaaaca
     4021 gtcaatctcc aatacagtcc gggaatccac ttgatcagta ttacgcatga ggaccaagcc
     4081 tcacgctcag agagtacgag gctccagctg attccaatgt cccgggggtt ggccactgag
     4141 gacattggtg actagccctc gatcgtctta gcttgtttta ggagtaggtt gtatgcaacg
     4201 taagtctatt tggcagtttt tgaaaactac tatcaacgaa tggaccgaag cagaaccgtt
     4261 ccagctgtca gcggctttat cgtattacac tctattttct ctcgctccac tgctgctgat
     4321 cgctattgga gtcgctggcc tcgccttcgg cagagaagcg gcgcagaatc agatcgtgga
     4381 gacgctccag gggatgatcg gacaagacag cgcgaaagcc gttcaggaca tgatccaagc
     4441 atcgaatgag aaaccgaaaa ccgggatagt gtccacggtc attggtttcg tcgtgttatt
     4501 gtttggcgca ggcggggtcg taggccagct gcaaacttcg ctgaacaaaa tatgggaagt
     4561 aactccgaag ccgggccaag gaatttgggg ttttttgcgg cagcgcttct tctcttttgc
     4621 gatggtgcta gcaattgggt tcttgctgct ggtatcgctc gtcgtgacgg cggttttgag
     4681 tagcttcacg gccatgctga atagctattt agggaacgct acgttcgttg ctcatgcgat
     4741 cgatatcctc gtatctttcg gatttgtcat gttgctgttt gcgctgattt acaagtacgt
     4801 gccggacgta gatatccaat ggcgggacgt gtggatcggc gccgcgatca catcaatact
     4861 gttcaccgta gggaagtttc ttatcggact ctatatcggg acgagcggcg tcagctcgac
     4921 cttcggcgcc gccggttcgc tgattacggt tcttgtttgg gtctattact cgtccttgat
     4981 cttttttctt ggcgccgaat ttaccagagt gtatgccacc caatatggat ccggcgtggt
     5041 ccctgctgaa aatgcccaac cgatggatgc tgcgcctcaa tccgcgaacg cgtgaaatga
     5101 atcgaataga gacgttcggc cgtagcagaa gtaaactcgg ctgatcaagc gacggcatca
     5161 gagcggcagc gaacaataaa taccgtccct tttccgggtc ggtagtgtaa accacgcttc
     5221 cgtgatgttc agaaacaatt tgctcgacaa tgatcgaaca ggcttacccg ctttgatgtc
     5281 ctccggccgg taaaagcaag agaaatgttt ccgatgattt cttcctttta tgcgttgcgc
     5341 gccggagttc caggtcgcaa ccaagccatc cgtattcaac atgtaaatgg cataatcacg
     5401 aacgctctcc accaacagcc taaattgctc ttcgggcgga ggtcaattgg ctagtttaat
     5461 gcccaacggt acaccgttag ccaaaagacc acgcacgttc ctagaataat agcccagccg
     5521 gcgagccact gcggaagtga aagcggattt tccaattcgc gatgtccgtc catggtagac
     5581 ctccactggt ggtgttggaa ggagcataca ccaagggcgt ataaacgccc ggaggttagc
     5641 ggctgcaatt tccgcgcctc aacgtatcga aaaaagaaaa ggaaagttgc gaacgatgcg
     5701 tattagtttt tcgcagaggt agagaagttc gccatgatgc gggaggttaa cgatttacct
     5761 ggcggtggtt cggctacctc ccgctgacgc tttgggtgga accccctgaa gatttcagga
     5821 ggctgatcag cggatcggga tcaggaatat tttcctggca tccctttgag catactgcct
     5881 tcctatcttc gggcagtgca cccttttttc ctttagctcg gcaatattcg gaggaggatc
     5941 cttattgaca aaggcttcct caatgctttg tttgctggct ggccctccat ttaagctaac
     6001 agtaacccca gtcgtatgca gatcaccaca ggcattgcac acggcacaga cgttatacat
     6061 agccatgctg gtaccctccc gttcgcaatt ccagctaagg acacaatcgc ctgcttgtcg
     6121 ccgcgtttag tctttgcttg ctaggccatc tacccccgtt tcagataaag ttcgtccaag
     6181 tctacttcgc gcgtctcgag cccaagttcc agcgcctttt tgttgcatgg gtgacacacg
     6241 agcatgttga gcagtatccc gcccgggtcc tcgctgatta ctcgatatcc tttgcttgta
     6301 agcgttcgct cacagtggtc gcaaaatagc tccctcggca tgtagttcga ttgagcaatg
     6361 cacatgccat tcagatgttt ctaaattctt aaacgagaga tcttgtttgt actcatgaac
     6421 tgtgagcgat ttagtttgct tagatcctgg cgtcgcttta ttgcgacttc attctctatt
     6481 ctggagtgta ggctcgagct tcaccagctc cgaaaaccaa tggtttgtcc tcttttccgg
     6541 tacgtgtgga gttggccgag acgcacatag aaacagacag aagtggccgt ttgtcaaacc
     6601 cgaattcgta gaaggagcca gagagttaga ggctagtagc ggtgggtcgc agcaatggaa
     6661 aaatcgatcg cagcgggtga gttgttcttc cgttaagttt cggtaaaaag cccggtcgat
     6721 cgacggattt taacaggcaa aaaactgtca acgaatcgtg aaacattttt gcgagtggca
     6781 gggtggtcct taacaataag gatttttgcg ccatcgttta agtcccttca gtgtcaactg
     6841 tcatccctcg gccaacgtac gtaggtcacc ataggtgagc agggttcaac agttttgcag
     6901 ctgagtgtcg ttgaccatga aagtttaagc ggctgttatg tttcgagagg caccactggc
     6961 caagcgacca cctcttggaa gcgcgcgaag tgagccagcg gtttctgttc cggcagatca
     7021 attccgatgg agtgcgtcat cgtgttcacg tccagcttca cttcggccgg ctgcagcgcc
     7081 catggtggat gatgaatttc gccaaacacc acatgatccg gcttcaaccc ggcgaacagg
     7141 caataccttt cagtgagcca atgatcgagc gtccccgcag tcgatcgaaa aaccgctccg
     7201 ctaggtccgt actcagcgcg gaactccgcg cgcggggaat tcttgtggat gcgtacgctg
     7261 tcataccgga tcgtatccgt cgcagattcg actttcatgc gcgcatcaaa atatggcagg
     7321 ccaagccatc gcgccgctct gaccgcgatc caactcgcag cgtcgaggct gaaaaaccag
     7381 acgccgcttt tccctgctga ttttacatag gtgcggacgt ttagttccgg caatgtcaga
     7441 ggcagcggga cgtaacgagg ccgcacgccg ctcatgtgga aaggaatgat tccaacccat
     7501 gaccatcgat caaaggtatc gagctcaagg atcgtgggaa ttagcggcct gatcaattca
     7561 ggccgaatcg gccaatggag aaacagcaga tcatgccagc gcatgaacat catccagcgg
     7621 gattttggca gtggccaagg ccggtgcgac gtttgatccg ttatcggcgg cacgcgcaaa
     7681 ctgcaacccg aatttactgt cgcacccgcg gtgattgcgc catgttcgat gaactcccgt
     7741 cgattcatag ctcggtacgc tcacagccat tgtcaagttg acgcgagcgc cccgtagtgt
     7801 tcacaagcgc gcctccgcct ggacacttgt gcctatgatc cgctgggcaa tccactgcaa
     7861 taggaatgat gtagaagtcc tctgccacgc cgcatccgac aagccgtgat ctgcgtcttt
     7921 gatcaaccgg tgcgttaccg atcgtgcccg agcgaaggca gccaagtaat tagcgattac
     7981 cggatgcggt acgacatcgt cctgctcgga ctcgacgatc agcacattac cggcaaacgc
     8041 cgcgcacgcg cgcagcgctc gattttcctc cggagctacg gcgcgcctgc ggtaggcgca
     8101 aaaatccgga tcttcatgaa gcttgcgctt gggcaactcc cagccggcat cgttgtagag
     8161 tgccggcgcg cgcagcgcta gccatcgcac tgatcgctgg cgcgtcatga tggcggcgag
     8221 atatgcgcca taactgctgc cgacgactcc tagggctgat gtgtcggcac ccggttggct
     8281 tgccagtgca tcgtaggcgg ccaggagatc tcttaaattg tcttcgcgcg aaaccagttc
     8341 gcgctgccca tcgctacgcg cgtgaccgcg cagatcgaac gttaaacaga taaaccccag
     8401 ctcggctacg gcctgcgccc gattgaggta ctgccgttga ttgccgcccc agccgtggac
     8461 aaatagcacg gcgggcccag cgctgacgcc ggtgatcagc cgaccctcga tcttttgatc
     8521 atcaaccgca atctcgattg gctcatcgtg gattgtcata cgcttcgacc tgagagtatt
     8581 tagtgatcgg tccgacccga tcgtcgacac cacggaaatg cacgaccgca tccaacggcg
     8641 tttgcccgtc gccgtaaagc tcgatcgacg ccgcgcgcac cgagcgaagg ccgggatcgg
     8701 cgcgaaaagc ttcgagtgcg gcaatctccg cagcgctggc gccgccgata cgccaggatt
     8761 gttcgagcac gccgcatcgc tggcgcccct catcatcgac aacctgcgca acatcgtagt
     8821 tgcgccggga gcataaccat cccgggaagt tgttaattgc cgcagcttcg tatgttcgcg
     8881 cgttgtttat cgccgaccgc gcctgtggcg ggagctcgag gctgagcaga tcatcgtagt
     8941 cgccccgcac cacggtcaag tccgagccgc cataaacatt ttcgtttcgg ttgtccctgg
     9001 tcagtaactg cctgccatag tacgtcgccg aaagatccgc cacaaaagtt tgcccgacac
     9061 tgtaggtttc gatgtgatcg aaattctgtt cgatcacggc cccgtactgc gcgagttccg
     9121 ctggatcgat ggtatcgaga agcgcatcga gctcatcact cgatttgatg accgtctgtc
     9181 ctaggccgcc aatgccgcgc gccggtttga gacgggcgtg gccgcgcgcc agtactcttt
     9241 gcgcggcgcg gcgcagatca tccggtgcga aggcggaaaa accaaacagg actacatccc
     9301 gaacactgcg cgcgaattca tgcgaccaac ctgccggagc gaaagcatgg ggcgatacca
     9361 gggggtgagt gatcgtcttg gtcgcagcga acgggtacgg aacaacgccg ccaaacaagt
     9421 catggtgatc gcgaatatca acttttcgag ccagctcgga tttagtgaga gtgtcgttcg
     9481 gaacgaagta aagtggaccg gcatagtggg tgtccgggtt atactctcca gcgaattcat
     9541 agcccttcaa tgccgcgagc ttccgggcaa gcagcattcg cgtgacgctc tcgtgtcgcg
     9601 ccggcgcatg ggatgatggg tcagtatact gaaccacggc gccaagcgcc cacagcgcgc
     9661 gattgagcac tcggtttctt atgacgatca tcccgcttct tcttgcagcg tttaacaatt
     9721 gttggcgatg ctcgcatgtt tcatcgtcgg tgtgagcagc gcccggacag aactgactaa
     9781 cgtagcgtcg cgcaaaagga gggccattgc actcacaaac gactggacca gcaagacagg
     9841 gtcgtcctcg aaaatggaac acgaggctga acgcgatcaa tcgcggaatt gaaacaacgc
     9901 tactcttcga tcgtatccgg tttgggacgg ccgattacaa cgatggcttt cgatccataa
     9961 actaaaacgc cagttgtcag tcatggtgca ccgcccgcat cagattgtcc gcgcagaaaa
    10021 cctttgaacc agtgtatgta cctcgaacaa acaaccctgg tgggacgtga gatgcactct
    10081 ctgaaatcct aaactgtcgg catcgccgcc gcccgttagt aatatgcctt ggtggcatat
    10141 ttgttgctga ccaagcttct aacgcgcact gaaacaaaca tcacgtcatt atcttttccg
    10201 cgctttcaaa aacttggttc tgtcccgaaa gttagccaca acagggataa gaagccgagg
    10261 ggaactgagt tgttgaatgt ctagaatcct aatcaccggc ggggctggat ttatcggatc
    10321 gcatttagcc gacgagcttc tgcacagtgg ccatgccgtc aaaatcttcg atctattgtg
    10381 ctcgcaagtg cacggcacgg aaaatcaacc gcctgaatat ttaaatcctg acgtcgaact
    10441 cgtcctcggc gatgttcgcg atcgcgctgc ggtcgaccga gcgttgaagg gaatcgatgc
    10501 agtttatcat ttcgctgcgg cggtgggcgt cggccagagc atgtatgagg tcgagcacta
    10561 cgccagcatc aatgatatcg gtacggcggt gctgctcgag gccctgatca aacgtcccgt
    10621 cgaacggctc atcgtcgcat cgagtatgag tgtttatggg gaagggcttt atcgctcgcg
    10681 ccgtggcgcc attcattgtg ccgtcgagcg cagtccaacg cagctgcgct cggcacgctg
    10741 ggatcccgtg gatgacgatg gcgtcgccct agaaccgatc ccaacgccgg agagcaagac
    10801 gccggcgctc agttcggttt acgcgctgtc gaaatatgtc caggaacgta tgtgtttgat
    10861 gatcggccgc gcctacaaca ttccgacggt cgcgctgcgg tttttcaatg tttacggacc
    10921 gcgacaagcc ttatcgaacc cgtacaccgg tgtgctggcg atttttgcct ctcgtttgct
    10981 caacggcaaa gcgccgctga tcttcgaaga tggcgaacaa caacgcgact tcgtcaatgt
    11041 tagagatgtg gtgcaggctt gccggttgtc tctcgatgca gcctcggcgc cggacagagt
    11101 tttcaacatc ggcagcggcc aagccttcac gattaccgaa ctagctgaac ggatggccga
    11161 tgccctcggc aagaatgtcg agccgcaaat aaccggcaaa tatcgcgtgg gcgatatccg
    11221 ccactgtttc gccgatatct cgcttgcccg atcgatgctc gattacgcgc cgcagatttc
    11281 gctggaggac ggtgttttgg aactcgcgga ttggctcgaa gggcagattg ccgacgatca
    11341 cgtcgatagc gccagcgccg agctggctgc ccgaggattg accctgtgaa gaaggcgaga
    11401 tccggccgaa acggcgcagc aggcatcaaa ccggatgtct gcaagcatcc gagcttacgc
    11461 gcccaaatcg ccgaggcgga cagcggcgca ggagtgcgga tcggagtcgt tgaaagcttt
    11521 cgcatcggtg atcgtgctgg ggttgaaaat gttctggccg acttgcgcga gctcagagtc
    11581 gccgatctgc gtaccacgat atcgtgggcc gattgggaca gccctgaagg cgaggcctgg
    11641 tacagttggc tcttgccgca tttagccagg caattgaatg taattccctg cgtcctctac
    11701 acaccgttat cgcaagccgt ggcgccgcgc gtatcagcgc cgccttggga tcccaaggcc
    11761 tacggtgatt ttatcgacgt actgatcacc cggctgggca gttgtttcga atggatcgag
    11821 ttatggaacg agcccgatcg gctaagcgag tgggaccgca ccctcgatcc ccattggcaa
    11881 actttctgcg ccatgatcgg cggcgcggct tactgggcgc aacgacgcgg caagcgcacg
    11941 gtgctggcgg gactcaatgc ctttgatccc aactggctcg agttgatgtt ccgtcgcggc
    12001 gtgatgcagt acatcgatgc cgtcggtgtt catggatttc caggcacctc ggaggccaat
    12061 tgggagagct ggcccgcggt gattcagagc gtgcagcaga ttctgacgca gcaccgttcc
    12121 ggcgccgaaa tctggatcag cgccacggga tattccacct ggcgccacga tgagtacgaa
    12181 caattgcgcg ccttcgcgcg ggctctcgag gcgccggtgg agcgacttta ctggcacgcg
    12241 gcgcgcgatc tcgatccgga attttcgacc cgtgacggtt ttcatgccga cgatcgcgac
    12301 tatcactttg gactgcgccg cagcgatggc agcgagaaat tattggcgcg gctgtggggc
    12361 gagggcggct tggacgccgt ctggggggcg ctcgagcctc atccgggcgc gcgcggtagt
    12421 gctgccaagc caacccagcc agtggtgatt accggcggcg ccggttttat cggcaccaat
    12481 ctggcggatc gtcttgccaa cgaaggcgag cgcgtgctgg tgttcgacaa tctgtcgcgg
    12541 cccggtgttg agcggaacat gaaatgg
//
LOCUS       DASUVS010000445        11494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_417498, whole genome shotgun sequence.
ACCESSION   DASUVS010000445 DASUVS010000000
VERSION     DASUVS010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11494
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417498"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..3067)
                     /locus_tag="VF182_23315"
     CDS             complement(305..3067)
                     /locus_tag="VF182_23315"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX6440065.1"
                     /translation="MMPQISEIPSLNPAQLKPKSGVKVSRYFTRAGINPYDEIDWEIR
                     SASITNEKGKVVFEQSNVEIPKAWSLMATNVVVSKYFRGPIGSPQREYSVRQMIGRVV
                     DTITRWGAKDGYFASDDDAQNFHDELTHILVHQKACFNSPVWFNCGIEEKPQCSACFI
                     LSVDDTMESILDWYRKEGIIFKGGSGSGVNLSKIRSAKEQLAGGGTASGPVSFMKAAD
                     ASAGVIKSGGKTRRAAKMVVLNVDHPDIEDFVKCKVEEEKKAWALIEAGYDSSLDGPA
                     YGSVFFQNANNSVRVTDEFMQAVQEDREWKTRFVKSGEVSDTYKACDLMRMIAESTYF
                     CGDPGMQYDTTINDWHTCSGTGRINASNPCSEYMHLDNSACNLASLNLLKFLREDGVF
                     DTESFRRAVDIIVLAQDILVDNSSYPTAEITANAHAFRELGLGYANLGALLMSLGLPY
                     DSDGGRGYASAVTALMSGEGYLQSARIAAATGAFAGYDVNRQPMLRVLNKHRSHAYKI
                     SSSHVPLDLLTAARESWDQTCAEAETHGVKNSQISVLAPTGTIAFMMDCDTTGVEPDI
                     ALIKYKKLVGGGLLKIVNNTVARALKRLGYDVKQIQDIVEYIEEHETIEGAPHLRDED
                     LPVFDCAFKPANGIRTINYMGHIRMMGAVQPFISGAISKTINMPNEATVEDIIQAYME
                     SWKLGLKAVAIYRDGSKRTQPLNTSKDKDETKTSAAENREARPLRRKLPDERRSITHK
                     FDIAGHEGYIIAGMYEDGQPGEIFITMSKEGSTISGLMDSFATAISIALQYGVPLRGL
                     VDKFSHMRFEPSGFTKNPDIPMAKSIMDYIFRWLATKFLDGDAQREIGVVKQETEDKE
                     IAVSKKVVSIAAQTPRGGAPVTSISSVTSLYQQDAPPCPDCGAIMIRSGACYKCLNCG
                     AVSGCS"
     gene            3295..3768
                     /locus_tag="VF182_23320"
     CDS             3295..3768
                     /locus_tag="VF182_23320"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX6440066.1"
                     /translation="MGKKTAQSIDRVGEVLSNSLKRLDLSFRLSEYGVWPVWDEVVGK
                     TIAANAQPEKIRNGTLFIKVSSSVWMQQLQFMKDMIAEKLNQRLNREVVKNIFFVVGR
                     IDAPGLAEKVAEASTPAVEEDPKIDPQFLDSVSDPELRQAFKKLLKSYSRRRPQE"
     gene            complement(3816..4649)
                     /locus_tag="VF182_23325"
     CDS             complement(3816..4649)
                     /locus_tag="VF182_23325"
                     /inference="COORDINATES: protein motif:HMM:NF017755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX6440067.1"
                     /translation="MKKIFAISEIDAESHPERLRRLSGETLSSAALAPDETLDALFGG
                     DLQLYQKKQGYRYSIDAILLADFATIRSAARVIDLGTGNGVLPLILAYRHPSILVTGV
                     EIQRQMAERAARNIRLNGYQDRIVIAQMDIASTMERFKPESFDSVICNPPYRQATSGR
                     LSLSSERQIARHELKATLDDFVRAAAFLLKNKGSFACIQLGDRTVDLVGAMRSAGLEP
                     KRLRTVHAFSDAKASLVLVEAVKAGRKNIDILPPLIIYDSAKNYTAELSAIAAGHKRP
                     F"
     gene            4720..5490
                     /locus_tag="VF182_23330"
     CDS             4720..5490
                     /locus_tag="VF182_23330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HEX6440068.1"
                     /translation="MVPFLGRIGRYAIDQIAGMGRMFLFLISAILQAFRAPAKLRLIV
                     YHIKTIGVDSLSVVALSGFFTGMVMGFQGYYSLRQFNAESWLGSATALSLLRELGPVL
                     SAFMVTGRTGSAMAAELGTMRATEQIDALYSMAINPIKYLVTPRIIASLIAMPMLTAI
                     FNVIGIFGSYAVGVGILGVSSGSFFSGMENSVVFHDVYSGMIKSLCFGLIITWICCYK
                     GFHAPPMATGVSQATTESVVLSFVFILVWDYFLTSVML"
     gene            5494..6240
                     /locus_tag="VF182_23335"
     CDS             5494..6240
                     /locus_tag="VF182_23335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6440069.1"
                     /translation="MIQVVDLYKAFGRQQVLRGVNLDFAKGKTTTIVGTSGCGKTVLL
                     KHLNALLLPDRGEVLIDGIDITKLGQKELYQIRAKFGVLFQGAALLDSMTVFDNVAFP
                     LREKTRMTEGEVKTKVADRLEQVGLGEMGHKLPAELSGGMRKRAGLARALIMDPEIVL
                     FDEPTTGLDPVLATSIHQLISRTQHDFGYTGIIVSHTIPQVFEITDYVVMLASGVVEE
                     VAPPEAFSVSESPIVQQFIHGRTEGPIDVL"
     gene            6250..6711
                     /locus_tag="VF182_23340"
     CDS             6250..6711
                     /locus_tag="VF182_23340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990531 - phospholipid-translocating
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005548 - phospholipid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEX6440070.1"
                     /translation="MNHSRTDIIVGIFVLAGAICLGYLAIRLGKVELFGTTGYVVYAD
                     FSSVAGLKVGDPAEIAGVRIGKVESLGLVDDRARVALRVDPDVELQDDVIASVRARGL
                     IGDKFVLITPGASDNIIAPGGKIRETESPQDITDLIGKFVGGDLTSKKDEQ"
     gene            6708..7322
                     /locus_tag="VF182_23345"
     CDS             6708..7322
                     /locus_tag="VF182_23345"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440071.1"
                     /translation="MTKSVRHLVVLSLLMVFAGPVASMAGPPTDQIKATVDNAVGLLR
                     ESRQNAGAPQDRRDQLRQILFTRFDFSEMARRALGAHWRRRTAAEQQEFVQLFTELLE
                     RQYAGIIESYTDEKIVYISERIDGAFAEVNSKIFTAKGQELSINYKAQLVGQEWKVYD
                     VIAEEISLVNNFRSQFNRVISSSSYDELIRRLKEKQADVIPVKN"
     gene            7492..8427
                     /locus_tag="VF182_23350"
     CDS             7492..8427
                     /locus_tag="VF182_23350"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6440072.1"
                     /translation="MAINIDWHSHHTALEVSDRIKDVTGKRPHIDALDSPDFSKRIRE
                     MDEAGVDLQLICQGAGVYADQMPPEQALEIVRMSNSVLAERLERYKDRLMGVTALSLK
                     NIAASVAEIERTAKQSFRAVLLYPHVEGKMLVDTPEMDPIFAKISELNLPIFLHGAGT
                     AQDPSLARLEDGGAGVAYAVLSDGEVAECCLRMIASGLFDRYPNLKIVIRSSGGGIPL
                     LLHRFFWKHKGPNGERRYSEIFLEHFLIDCASSDARTLSFLIDKMGENNMVFGSDYCG
                     GLDRLNRSFNAILAQPNPDHIKGFMEKNSRQLLHI"
     gene            8498..9727
                     /gene="lhgO"
                     /locus_tag="VF182_23355"
     CDS             8498..9727
                     /gene="lhgO"
                     /locus_tag="VF182_23355"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEX6440073.1"
                     /translation="MASLYDLTIIGGGILGLATALKITAAYPRLSLLLLEKEPELARH
                     QTGNNSGVIHSGLYYRPGSLKARTCVAGRKELIQFCDDNAVPYEICGKVVVATTREEL
                     PRLEELHRRGEANGLRGLELIGSERLKELEPHATGIKGLHVPETGIIDYKKVAAAYAS
                     NIRDFGGDLRLSHRVVGMIERADEIVLQTSGGDYRTKHLINCCGLQSDLIAQMARRAN
                     QDGGPEHRIIPFRGEYYKIAPERQFLVQNLIYPVPDPTFPFLGVHFTRMAKGGVEAGP
                     NAVLAYAREGYRHSQVNLKDLWQTVSFRGFWAMTGKYWQTGFGELYRSLSKAAFVRAL
                     QKLVPDIRESDLVPGGAGVRAQAVSANGALVDDFVIKEGRNAIHVLNAPSPGATASLA
                     IGRQICEMAEKNFDLRN"
     gene            9889..10416
                     /locus_tag="VF182_23360"
     CDS             9889..10416
                     /locus_tag="VF182_23360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX6440074.1"
                     /translation="MIIEYKGKKPSVSSKAFIAPTAVLIGDVSVGDDASIWWGAVLRS
                     DQGGYPIVIGPRTSIQDNCVMHSGEATTIVEEDCTVGHGAIMEGCVIKRGAIIGMNST
                     ILENTEIGEESLIAAGSVVVPHTKIPPHVLAAGTPAKVKKAIEGEALKWIKMGASTYV
                     ELCRSYLGGDYKIVG"
     gene            10443..11357
                     /locus_tag="VF182_23365"
     CDS             10443..11357
                     /locus_tag="VF182_23365"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX6440075.1"
                     /translation="MRIILIGQAAFAEKTLDKLVSKGEEVLAVFCFPDTPGGKIDPVK
                     QRALQLGIPVHQHKTLKTPEVIEQFVSLKADLAILAFVSQIIPPPVFNAPRFGSICYH
                     PSLLPKYRGRTAINWALIRGETVTGLTIFWLDKGIDTGPILLQKEVQVAPDDTTGTLY
                     FNKLFPLGVEAIGEAVDLIKAGNPPRIVQDESKATYDPPCGDEHAKIDWSKPAQEVYN
                     LIRGCDPQPGAHTAYQGKTLRLFDARLQKNLNGAEPGQVSGVGDEEITIAASGGTLTV
                     KRVRGEAGKISGAEFAKQVGLEVGDRLE"
BASE COUNT         2625 a         3114 c         3082 g         2673 t
ORIGIN      
        1 ggcggaatgg aagtgaaaaa tcttaccacg gcgattcacg atgcggtgcc cagcgcccag
       61 gtcgctggtc ttctcggtct caacttcctg agcaactttc gtctggatat agataccgag
      121 aagggcatac tccatttgga acagaaataa tcatcgccgt tgctccaagt ctaagtcgcg
      181 ctttcagaat ccctctccga ggtgagtctt cccatacatc ggttatggct tctggctcaa
      241 aaataaaggg gcggtggatt ttaccagcgc cccttgttcg tgccggaatt tgacgcaggt
      301 gatctcaaga gcaaccgctg acggcgccgc agttcagaca cttatagcag gcgccgctac
      361 ggatcatgat ggcgccgcag tcgggacagg gcggcgcatc ctgctggtaa aggctggtga
      421 cactgctaat gctggttacg ggcgccccgc ctcgtggggt ttgagcagca atcgacacga
      481 ctttcttaga gaccgcgatc tctttatctt ccgtctcctg cttcaccaca ccgatctccc
      541 gctgggcgtc gccgtcgaga aacttggtcg ccagccacct gaagatgtaa tccataatcg
      601 acttggccat gggaatgtcc ggattcttgg taaaccccga gggttcgaaa cgcatatggc
      661 tgaacttgtc caccaaccct cttaacggca caccgtactg cagcgctatc gaaatcgccg
      721 tagcaaaaga atccatcagc ccggaaatgg ttgagccttc cttggacatg gtgatgaaaa
      781 tctctccggg ctggccgtct tcgtacatgc ccgcaatgat atagccttca tgcccggcga
      841 tatcgaactt gtgagtaatc gagcgccgtt catcgggcag cttgcgccgc agcggccgcg
      901 cttccctgtt ttcagccgcg ctggtttttg tctcgtcctt gtccttcgac gtgttgagcg
      961 gctgcgttcg tttcgacccg tcacggtaaa tcgccaccgc tttcaatccg agcttccagg
     1021 actccatata cgcttgaatg atgtcttcta ccgttgcctc attgggcata ttgatggttt
     1081 tagagatcgc gccggagata aacggctgca ccgctcccat cattctgatg tgccccatgt
     1141 aattgatcgt gcgaatgccg ttggcgggct tgaaggcgca gtcgaagacc gggaggtcct
     1201 cgtctctgag gtgcggcgcg ccttctatgg tctcatgctc ttcgatgtac tcgacaatgt
     1261 cttggatttg tttgacgtca taccccaagc gcttgagggc acgagccact gtgttattga
     1321 cgatcttcaa gagaccgccg cctaccagct tcttgtattt gatcagagcg atatcgggtt
     1381 ccactccggt ggtgtcgcaa tccatcatga acgcgatggt gccggtgggc gcgagcaccg
     1441 agatctgcga atttttcacg ccgtgggttt cggcctctgc gcaggtctga tcccaggact
     1501 cgcgcgccgc cgtcagcaga tccaacggca cgtgcgacga cgaaatcttg taggcgtgag
     1561 agcgatgctt gttgagcacc cgcagcatcg gctgtcgatt gacatcgtat cccgcgaagg
     1621 ctcctgtggc tgcagcgatg cgcgccgatt gaagataccc ttctcccgac atcagcgcag
     1681 tcaccgcaga ggcatagccg cggccgccat cggaatcgta gggcagcccc agcgacatca
     1741 ggagcgcgcc aagattagca tagcccaaac ccagctcgcg aaaggcatgg gcattcgccg
     1801 ttatctccgc ggttggataa gaagaattat cgaccagaat atcttgcgcc agcacgatga
     1861 tgtcgacggc gcggcgaaaa gattcggtgt cgaaaacgcc gtcttcacgc aggaatttca
     1921 gcaggttcaa ggaagctaaa ttgcacgccg aattgtccag atgcatgtac tccgagcaag
     1981 gattggaggc gttgatccgg ccggtgcccg aacaggtatg ccaatcgtta atggtggtat
     2041 cgtactgcat tcccggatca ccgcaaaaat aagtgctctc ggcaatcatg cgcatcaaat
     2101 cacacgcctt gtaggtgtcg ctgacttcgc cgcttttgac aaagcgggtc ttccattcac
     2161 ggtcttcttg caccgcttgc ataaattcat cagtaacacg gacggagtta ttcgcatttt
     2221 ggaagaatac gctgccgtag gccggaccat ccaagctaga atcgtatccc gcttcgatca
     2281 aagcccaggc ttttttctcc tcttcaactt tgcacttaac aaagtcctcg atatccggat
     2341 ggtcgacatt gagaaccacc atcttcgcgg ctcgcctagt tttaccgcct gatttgatca
     2401 ctcccgccga ggcatccgcc gctttcatga aggagaccgg accggacgcg gtgccgccgc
     2461 cggcgagttg ctctttagcg gagcggattt tcgagagatt tactcccgaa ccggaaccgc
     2521 ctttgaaaat gattccttcc ttgcggtacc aatcgaggat agattccatg gtgtcatcga
     2581 ccgacagaat aaaacaggcc gagcactgcg gcttctcttc gatgccgcaa ttaaaccaca
     2641 cagggctgtt aaagcaggcc ttctgatgga ccaaaatatg cgttaattca tcgtgaaaat
     2701 tctgcgcatc gtcatcgctg gcgaaatagc cgtctttggc gccccaacga gtgatcgtat
     2761 cgaccactcg gccgatcatc tgccgcacac tgtattcccg ctgaggacta ccaatgggac
     2821 ctcgaaaata ttttgacacg acgacattgg tggccatgag cgaccaggct ttaggaattt
     2881 ctacgttgct ttgttcgaaa acgactttgc ccttttcgtt ggtgatcgac gcagaacgaa
     2941 tttcccaatc gatttcgtcg tacggattga tacctgctcg ggtaaaatac cgacttactt
     3001 taacgccgct tttgggcttg agctgggccg gatttaagct aggaatttcg ctgatttgtg
     3061 gcatcatggt cgccatggta gtatctccct tcggtcggta aatatcaata tgtagtgtga
     3121 aatgcggcgg aatgcactag attttgtata gttaaaattt ttggcaattt ttgtcaagat
     3181 ttttttttga gcgcttgttt tcactagaat ttatcgaact tctgtgacca tgacggaaat
     3241 aaaaacgcta gaatcgccgc tctacgtttg ctaaattcgc cgctgagtta cttgatgggg
     3301 aaaaaaacag cgcagtcgat agatcgggtc ggcgaggttt tgagcaactc tttaaagcgt
     3361 ctcgatctct cgtttcgatt gagtgaatac ggtgtctggc cggtgtggga cgaagtggtc
     3421 ggcaagacga tcgccgccaa cgcgcagccc gaaaagatcc gcaacggcac attgttcatc
     3481 aaagtgagca gttcggtatg gatgcagcag ttgcagttca tgaaagacat gatcgccgaa
     3541 aaacttaatc agcggctcaa ccgggaggtc gtcaagaata tttttttcgt tgtcggccgg
     3601 attgatgcac ccggactggc ggaaaaagtt gccgaggctt ctacaccggc tgtcgaagaa
     3661 gatccgaaaa tcgacccgca attcctcgac tcggtgtccg accccgagtt gcgtcaagcc
     3721 tttaaaaaat tgttaaagag ctattcgcgc cgtcgcccgc aggagtaatg atgcggcgcc
     3781 gaactcccgg cctgaataaa tccagattga ctagtttaaa agggacgttt atggccggcg
     3841 gcaatcgcgc tcagttccgc ggtataattc ttggcggaat cgtagatgat caacggcggt
     3901 aagatatcga tgttttttcg tccagccttg acggcttcga ccagaaccag cgatgccttg
     3961 gcatccgaaa aagcatgaac ggtgcgcaga cgtttcggtt cgagcccggc gcttcgcatc
     4021 gcgccaacta aatcgactgt gcgatctccc agctgaatac atgcaaaaga gcctttgttc
     4081 ttcagcaaga acgctgcggc cctgacaaaa tcatcgagag tggctttcag ctcatgacgg
     4141 gcaatttgcc tttcagaaga aaggctcagc cgcccgctgg tggcttgccg ataaggggga
     4201 ttacagatga ccgagtcgaa actttcaggt ttgaagcgct ccatcgtgga tgcgatatcc
     4261 atttgcgcga tcacgatacg atcttggtag ccattgagcc gaatatttct cgctgcgcgc
     4321 tccgccatct gccgctggat ctcaactccc gtaaccaaga tggacggatg acggtaggcc
     4381 aagatcagcg gcagcacgcc attgccggtg cccaaatcaa tgactctagc agcggaccga
     4441 atcgttgcga aatcggccag taaaatggca tcgatggaat accgatatcc ctgctttttc
     4501 tgatagagct gcaggtcacc accgaaaagc gcatccagtg tttcatcggg cgccaaagcc
     4561 gccgatgaaa gcgtctcacc agataatcgg cggagccgct caggatggct ttcggcatct
     4621 atctcgctga tcgcaaaaat ctttttcatg cttgcaaagc aaagcagagt tctatattag
     4681 tctgaatcca ctagcactgt tttttcctca acgaaaagca tggttccgtt cctaggccgt
     4741 atcggccgtt atgccattga tcagattgcc gggatgggaa gaatgtttct cttcctcatc
     4801 tcggccattc tgcaagcctt tcgcgcgccg gcaaaactgc ggctgattgt ttaccatatc
     4861 aagaccatcg gcgtggattc tctctcggtg gtcgcgcttt cgggtttttt taccggcatg
     4921 gtcatgggat ttcaaggcta ctacagtttg cggcagttca acgcggagag ctggctcggc
     4981 tccgccaccg cgctcagtct cctacgcgag ctcggtccgg ttctctcggc gttcatggtc
     5041 accgggagaa ccggctccgc catggccgcg gagttgggca ccatgcgcgc cacggagcag
     5101 atcgatgctc tttattcgat ggcgatcaat cccattaaat atctagttac tcctcggatc
     5161 atcgccagcc tcatcgccat gccgatgctc acggcgattt tcaatgtcat cggcatcttc
     5221 ggctcgtatg ccgtcggcgt cggaatcctc ggtgtcagct cgggcagttt tttttccggc
     5281 atggaaaata gcgtcgtctt tcacgatgtt tacagcggaa tgatcaagtc gctctgtttt
     5341 ggcctgatca ttacctggat ctgctgctac aaaggttttc atgctccgcc gatggctacc
     5401 ggtgttagcc aggcaacaac cgaatccgtc gtcctatctt ttgtcttcat cttggtttgg
     5461 gattatttcc tcacgtcagt catgttgtag gctatgatac aggtcgttga tctatacaaa
     5521 gccttcggca ggcaacaggt cctcagaggc gtaaacctcg atttcgccaa aggcaaaaca
     5581 acgacaatcg tcggcaccag cggttgtggc aaaaccgtgc tactgaagca cttgaatgca
     5641 ttactgttac cggaccgcgg cgaagttctc atcgatggaa tcgacatcac caagctcggc
     5701 caaaaagagt tataccagat ccgagccaaa ttcggcgtgc tttttcaagg cgccgcactg
     5761 ctcgattcga tgacggtctt cgataacgtc gcatttccat tgcgtgagaa aacaagaatg
     5821 accgaaggtg aagtaaagac caaagtcgcg gaccgactgg agcaggtcgg attaggcgaa
     5881 atgggacaca agttaccagc ggaactgagc ggcggaatgc gaaaacgcgc cggcttggcg
     5941 cgcgccctga tcatggatcc ggaaatcgtc ttgtttgacg agcctacaac cggtttggat
     6001 ccggtgttgg caaccagcat tcatcaactg atttcaagaa cccaacatga tttcggctac
     6061 accggaatca ttgtgagtca taccattcca caggtcttcg agattaccga ctatgtggtg
     6121 atgcttgcca gcggggttgt tgaagaggtc gcgcctccgg aagctttttc cgtgtcggag
     6181 agtccgattg tgcagcaatt tatccatgga agaactgaag gccccatcga cgttttgtag
     6241 gagatcgaag tgaaccattc tcgcactgac atcatcgtcg gtatttttgt gctcgcggga
     6301 gccatctgcc tcggctatct tgcgatacgc ctcggcaaag tagagttgtt cggcaccacc
     6361 ggttacgtcg tctacgccga cttttcttcg gtcgcaggcc taaaggtcgg cgatcccgca
     6421 gaaatcgccg gtgtcagaat cggcaaagta gaatcgctcg gtttggttga cgatcgggcc
     6481 cgtgtcgctc tcagggtgga cccggatgta gaacttcagg atgacgtgat tgcgtcggtg
     6541 cgcgcccgcg ggcttatcgg cgataaattt gtcttgatca cgccaggagc ttcggataac
     6601 atcatcgcgc ccgggggcaa gatcagagaa accgaatcgc cgcaagacat cacggatctg
     6661 atcgggaagt ttgtcggcgg cgatctcaca tccaaaaaag acgaacaatg acgaaatcag
     6721 tgcggcatct cgttgtgctt tcattgctaa tggtttttgc gggtccggtg gcatcgatgg
     6781 ccgggccgcc gaccgatcag atcaaggcga cggtggataa tgccgttggg ctactcaggg
     6841 aatcccggca gaatgcgggt gcgccccaag accgtcgcga tcagctgagg cagattcttt
     6901 ttacccgttt cgacttttcg gaaatggccc gacgcgcgtt gggcgctcat tggcgccggc
     6961 gcactgcggc cgagcaacag gaattcgtgc aactattcac cgagctgctg gaacgtcaat
     7021 atgctggcat catagaatcc tataccgacg agaaaatcgt ttacatcagc gaaagaatcg
     7081 atggcgcgtt tgcggaagta aacagcaaga tattcaccgc caaaggtcag gagttgtcga
     7141 ttaactacaa ggcgcagttg gtcggccaag agtggaaagt ctacgacgtc atcgccgaag
     7201 aaattagcct ggtcaacaat tttcgctccc aattcaatcg cgtaatctcc agttcctcat
     7261 atgacgaact gatccgacgg cttaaggaaa agcaggccga cgtgatcccg gtcaagaact
     7321 agccggacac gcgggctctt ttacctattt tctcaaagct gcagacggcc caaggctcaa
     7381 cttgcctgct ctctgcgcct ctttcaccga atcaccctat ccagtatgct ctagctgggt
     7441 ttattttttc ggagtagcag tttatagact gagctggaag ggagctgtcg catggcaatc
     7501 aatatcgatt ggcattccca tcacacggcg ctggaagtct ccgatcggat caaagatgta
     7561 accggcaagc gcccgcatat cgacgcgttg gattcgccgg acttttccaa acgcatcaga
     7621 gaaatggacg aagcgggagt cgatctccag ctcatttgtc agggcgccgg tgtttatgcc
     7681 gaccagatgc caccggagca agcgctcgaa atcgtgcgca tgtccaacag tgtgctggcc
     7741 gagcggctcg agaggtacaa agatcgattg atgggtgtga ccgctttgtc gctgaagaac
     7801 atcgccgcgt cagtggccga aatcgaacga acggcgaaac aaagttttcg cgccgtgctg
     7861 ctctatccgc atgtcgaagg caagatgctg gtggacacgc ccgaaatgga cccgatcttc
     7921 gcgaaaatct ccgagctcaa tctgccaatt tttctacacg gcgccgggac ggcgcaagac
     7981 ccctcgttgg cgcggcttga agacggcggt gcgggagttg cctatgccgt gctgtctgat
     8041 ggtgaagtcg cggagtgctg tctgcgcatg atcgccagcg gcttgttcga tcgttatcca
     8101 aacctaaaga tcgttatccg tagctccggc ggcggcattc cgctgttgct tcatcgtttt
     8161 ttctggaaac ataaaggacc taacggagag cggcgctact cggaaatttt tcttgaacat
     8221 ttcctcatcg attgcgccag ctcggatgct cgaacgctgt cgttcttgat agacaagatg
     8281 ggcgaaaaca acatggtttt cggttccgac tattgcggcg gcctcgaccg gctgaacaga
     8341 agcttcaacg cgatcctggc acaacccaat cccgatcaca tcaaaggctt catggaaaaa
     8401 aattcccgtc agctgcttca catctaattc gatcaggacg ctccgcggtg agttggcccg
     8461 agaggaatct gacggtggaa cttcacacta aatcctcatg gcatcccttt atgacttaac
     8521 gatcatcggc ggcggtattc tgggcctcgc caccgcgctg aaaatcaccg ccgcctatcc
     8581 ccgcctgagt cttttgctgt tggagaagga accggaactc gcgcgccatc agaccggcaa
     8641 caacagcggt gtgattcatt caggtctcta ttaccggcct ggctcgctaa aggcgcgcac
     8701 ctgcgtggcc ggacgcaaag agctgatcca attctgcgat gacaacgcgg tgccctatga
     8761 gatttgcggc aaggtcgtcg tcgccacgac ccgcgaagag ctgccgcggc tggaggaatt
     8821 acaccgccgc ggagaagcca atgggctgcg cgggctggaa ctaatcggca gcgagcgttt
     8881 gaaagagctt gagccgcacg ccaccggaat taaaggtttg cacgtgccgg agaccggcat
     8941 catcgactac aagaaggtcg ccgcggcata cgccagcaat attcgcgatt ttggcggcga
     9001 cctccggctc tcccacagag tcgtaggcat gatcgagcgc gccgacgaaa tcgtgctgca
     9061 gacctcgggt ggggattatc gcaccaagca tttgatcaat tgctgcggcc tgcaatcgga
     9121 cttgatcgca cagatggctc gccgcgctaa tcaagacggc gggccggagc atcgcatcat
     9181 tccctttcgt ggcgaatatt ataaaatcgc gcccgaacgg cagttcttgg ttcaaaatct
     9241 gatttatccg gttcccgatc cgacattccc gttcttgggc gttcatttca cgcgcatggc
     9301 caagggcggc gtagaggcag gacccaatgc ggtcctcgcc tatgctcggg aaggttacag
     9361 gcacagtcag gtcaacctga aggacctctg gcaaaccgtt tcgttcagag gattttgggc
     9421 gatgacgggg aaatactggc agaccggctt tggcgagctt tatcgctcgc tcagcaaagc
     9481 agctttcgtc cgtgccttgc aaaagttagt gcccgacatt cgcgaaagcg atctagttcc
     9541 cggcggcgcc ggcgtccgcg cccaagcggt gtcggccaac ggcgctttgg tcgatgactt
     9601 tgtcatcaag gaaggccgca acgctatcca cgtgctcaac gctccatctc ccggcgccac
     9661 ggcctcgctt gccatcggcc ggcagatttg cgagatggcg gaaaagaatt tcgacctgag
     9721 aaactagttc cggagtcgcg ttggccgctc atctacgggc atcccttgct aacattgctg
     9781 cttcatctca ttgcgccggc ttaaacgtct cgcgaaacac ctccaacctt gatttccacg
     9841 gccctgtcaa atatcgtggc gaaatattct gcggagggcc caatctgaat gatcattgaa
     9901 tacaaaggta aaaaaccgtc tgtttcgtcc aaagccttca tcgctcccac cgctgtgctg
     9961 atcggcgatg ttagcgtggg tgacgacgcc agcatttggt ggggcgccgt tctgcgctcc
    10021 gaccagggcg gttatccgat cgtgatcggt ccgcgaacca gcattcagga taactgcgtg
    10081 atgcattcag gcgaagcgac caccatcgtc gaggaagact gcaccgtcgg gcacggcgcg
    10141 attatggaag gatgcgtgat caagcgtggc gcgatcatcg gcatgaattc cacgattctt
    10201 gaaaataccg agatcggcga agaatcgctt atcgccgccg gcagtgttgt cgttccacat
    10261 accaaaatcc cgccgcatgt tcttgccgcc ggcacgccgg caaaagtaaa aaaagcaatc
    10321 gaaggcgaag ctctcaaatg gatcaagatg ggggcatcca cttacgtcga actttgccgc
    10381 agctatttag gcggcgatta caagatcgtt ggctagttaa gagcatcata cagggggaaa
    10441 gcgtgcgcat cattttgatt ggacaagcgg cgtttgcgga aaagactctc gacaaactcg
    10501 ttagtaaagg ggaagaggtg ctggcagttt tttgttttcc cgatacgccg ggcggaaaaa
    10561 tcgacccggt caagcagagg gctcttcagc tgggtattcc cgtgcaccaa cataaaacgc
    10621 tcaaaacccc ggaagtcatc gagcaatttg tttccctcaa ggcagactta gcgatcttgg
    10681 cgtttgtcag ccaaatcatt cccccgccgg tattcaatgc gccgcgcttt ggcagtattt
    10741 gctatcatcc ttcactgttg cccaaatacc gcggccggac cgcgatcaat tgggccctga
    10801 tcaggggaga aaccgtaacc ggtttgacga ttttttggtt ggacaagggc atcgataccg
    10861 gccccattct actgcaaaaa gaagtgcagg ttgccccgga cgataccacc ggcacattgt
    10921 atttcaacaa actttttcct ttgggagtcg aagcgatcgg cgaagcggta gacttgatca
    10981 aagcaggcaa ccctccgcgg attgtgcagg acgaatcaaa ggcgacttac gatccgccct
    11041 gtggcgacga acacgcgaaa atcgattggt ccaagcctgc tcaggaagtc tataatttga
    11101 tccgcggctg cgatccccaa cccggcgccc acactgccta tcagggcaaa accttgcgcc
    11161 tcttcgacgc ccgactacag aagaatttaa acggcgccga accaggccaa gttagcggtg
    11221 tcggtgatga agaaatcact atcgccgcca gcggcggcac cctgacggta aagagagtgc
    11281 gcggcgaagc cggcaaaatc agcggcgcgg aattcgccaa acaggttggt ctagaagtcg
    11341 gtgatcggct tgagtgattt cacatagatt tttcgcgcga gggttcacag gtttaactga
    11401 gttcatctca tcacgaagcg cacgaaggac acgaagctta cgaccgaaga cttactgttg
    11461 acttcatgct cttcgcgtcc ttcgtgataa gtcc
//
LOCUS       DASUVS010000446        10924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1947495, whole genome shotgun sequence.
ACCESSION   DASUVS010000446 DASUVS010000000
VERSION     DASUVS010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10924
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947495"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            426..1454
                     /locus_tag="VF182_23370"
     CDS             426..1454
                     /locus_tag="VF182_23370"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6440076.1"
                     /translation="MKSFAISLSLLLLGVTNLQAQTPFYEGKTIRIVVGLPAGDVYDL
                     YARMLAEHMGRHIPGNPNIIVQNMAGASSMITANYVYNVAKPDGLTFGSILPSLYFDQ
                     LVGRPEVKFDWPKFTWLGSFEKSNNLLYMRSDTPFKTIHDVTKANESPKCGSTGTGSP
                     SYYLVKLMNESIGAKFEIVTGYKGGQEIDLAVEKGEVDCRAFTVTTYFAREPFISWRK
                     KNFVRVLFQTGKTRDTRLPEVPTVQELIEQYKTPEGTKRMAALVLASGEFGRPIIATP
                     GIPADRVKMLREAFSKTLGDPALLADAQKRRLDIDPTSAEDLEALAKDVFTANRELIE
                     RMKSLLSK"
     gene            complement(1608..2531)
                     /locus_tag="VF182_23375"
     CDS             complement(1608..2531)
                     /locus_tag="VF182_23375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX6440077.1"
                     /translation="MKAVRIHRFGGLDVIRTEDIAKPVPNAGEVVVKVDAAGVGPWDA
                     LIRRGKSVLPQTLPLTLGSDFSGVIDSVGAGVARLKVGDEVFGVTNQRFTGACAEYAR
                     AKPSMIAPKPRALNYSHAASVPVVSVTAWQMVFDFAQLRAGQSVLIHGGAGNVGAYAV
                     QFAKQAGAMVITTAAANDVSYIRSLGGLGVIDYRASRFEDEVKDLDAVIDTVGGDILE
                     RSYGVVKHGGVIVSSSAVPSQEKAQQHGVRASFFLVEVTADRLQRIAELIDAGRLKTQ
                     VGEVLWLDEARHAHEMLEGAPHRRGKIILKT"
     gene            complement(2775..3692)
                     /locus_tag="VF182_23380"
     CDS             complement(2775..3692)
                     /locus_tag="VF182_23380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX6440078.1"
                     /translation="MSIDGELIGERYFHGARPTDPANLKSASKSIISTLIGIALDRGQ
                     LKSIKDPIAKFFPEHLGTTADPVKKTITTEDLLTMRSGLETTSNVNYGRWVTSGNWVG
                     HVLTRPMIDEPGGRMIYSTGNTHLLSAILTKATKMSTFEFARRYLAEPLGIAIRPWIR
                     DPQGIYLGGNDMHLTPRAMLRYGELYLNRGRLQEKQIVSANWVEESLKPRTQSSWSGR
                     DYGYGWWIDTLGGQTIHYAWGHGGQFIFTVPGLKLVVVTTAMPAPGEGRREHQGAIYD
                     LMERHLIPAAERKQLAAVSSTTSVEPQQN"
     gene            4226..5692
                     /locus_tag="VF182_23385"
     CDS             4226..5692
                     /locus_tag="VF182_23385"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEX6440079.1"
                     /translation="MLAPTSFAIAASAIREFRWAADPEGGAPFVEADPARPDRVVGFD
                     VEIAEIIARGLDRSATFMNIAFTSIDQSIERGDADIGLGGIEDTPARRRTMAPTVPYY
                     RFREVLSVRDADASRFRTLADLRGRRVGTLGGTIAYEILLRAERDFDIKAVSYDDDVH
                     PYTDLIIGRVDAVLLDNVLAERRRRTTSGFTIQPESVATGHYVGVLAARNAALRESVN
                     EVLRNAMRDGTLERIFRKWRVWNEDQPALFTNVLAGKPLAPVVGFDPIDGDAMLSKWR
                     TMRAYLPALFRAAAITMVLSCLSMALAVIIGVSIASGRIYGGKIARSLLTAYVEIIRG
                     TPILLQLFILYYGIVELIRLPAFIAALLGLGLNYAAYESEIYRGALEAVPGGQLDAAR
                     ILGFNELQVLTLVRGPQAFRLALAPMTNDFVALLKDSSLVSVLTVLELTKQTQIFATN
                     IGSWVVPGALCAALYMAMSLPLAALARRLEQRWRRHTA"
     gene            5671..6369
                     /locus_tag="VF182_23390"
     CDS             5671..6369
                     /locus_tag="VF182_23390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6440080.1"
                     /translation="MAQAHGMIANVLQLNDVRLRRAEREVLAGVTFSVPRGEIVALMG
                     PSGSGKTSVLRLIAGLEPFQSGSVQIEDLAMFANGGTPANTVRALRRKIGMVFQFHHL
                     FEHLPAIKNVWLAPVHAHGVPFHEAVRRAQELLALLGVEHRASALPAELSGGEAQRVA
                     IARALAVDPPLLLMDEPTASLDGARRSELRELLQRLVRQGRTLVLATHDEEFASVCAT
                     RILRMNNGRVSSQD"
     gene            complement(6591..7007)
                     /locus_tag="VF182_23395"
     CDS             complement(6591..7007)
                     /locus_tag="VF182_23395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440081.1"
                     /translation="MTNKNINRIGMTYARQSSNAPGASTLRTFIGKRTSIVVGLLLFP
                     MAAFSHPFHLTWTDMNADEDGTVIYREMSGVFHEVGFVGPNVTSFTDDPPGATGDTFC
                     WTVRAFRQGDISGSSNETCASVPAPSNPGQRRGKGE"
     gene            complement(7795..8121)
                     /locus_tag="VF182_23400"
     CDS             complement(7795..8121)
                     /locus_tag="VF182_23400"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX6440082.1"
                     /translation="MPDFNKAIANSELDEGSILAVRINGERIALYKVGGEVYATSEMC
                     SHSDCSLEDFGKIVDNDQVECICHGARFRIKTGEVTRLPAEIPLKTYSVKIDNDEIWV
                     RVSDPV"
     gene            complement(8248..9084)
                     /locus_tag="VF182_23405"
     CDS             complement(8248..9084)
                     /locus_tag="VF182_23405"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6440083.1"
                     /translation="MLKPKGLIAGHYECRSLQQTLPLLQDLLALEVVARTDSETVLKH
                     PNTDWKLIAHESGADAPDKPFQHHYGVRVATNREVDEAHQYLQSKKREYKIKIIQPRE
                     NHNAYSVHFFEPGGNFWEIESYEKADEAGMGKTTSPHWQTPINQEKFPGRGYVPQALT
                     HGTVQFHDIEATRRFYENVLGLEVVQLWPSSIYVKHPESPWYIVNLPFRAEPILPTRA
                     QRFVLALESASQTEEAHLWFSSSAQTLGLTTVDAIENANGSARFIFSDLNRNWWEVTS
                     TG"
     gene            9524..10600
                     /locus_tag="VF182_23410"
     CDS             9524..10600
                     /locus_tag="VF182_23410"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6440084.1"
                     /translation="MNHLRLTRVMMLFLLLATLTDYAEAQSGKTSSAGFDEKAVADFY
                     RGKTIRIVIGSGPGGTYDIYSRLISRHMSRFIPGNPTLLVQPRAGAGGLIAANAVYNV
                     EPKDGTVIGSFGETFPLRQAIGAPGIEFDSARYQWIGSAVNTAIACVGRTDSAVSSFN
                     DVTAGKPFTVGTMAPGSTIYDTPAIINAAFGTQMKLVRGFDGVANIVNAAESKEVDGY
                     CASWLAMLTTGRHLQLIHDGTLKPLLIMGDKTPDHALMKGVPAAETIAKTEEAKLLLR
                     AMHAPSEITNPYVVPAEVPKDRVEALRKAFSATFADPEFSSDSKKSKIEFSPSNGERV
                     TEVVQSILKTPPAILAKMKKILVQ"
BASE COUNT         2366 a         2889 c         3029 g         2640 t
ORIGIN      
        1 agcgtgtgtt tgtctttgtt ttgcttcaag tgagtccttc catcaaaccg ctgtgaggat
       61 tgtcttagtt actgtaagca gccaattttg tcaaatactt ttcggctcgg tgccggcgcg
      121 ttgatcgtgg tctgcgacag ttggccagcg attttgatct acccacctgc tgatcgcaaa
      181 tgcggtgtgc gtgtgactgc ccggctgagg cctgaattgg aatcgcccgc acggagcgat
      241 agagtcattt ggggcgtttt gattcctttc agttgacaca tcaatcccga atcgaataat
      301 cttggccgct cattaaggac aaacgttccg gttttgctct cctggtgctc cagaagatcg
      361 gcgaggtatc agccgaagga cgccgtaagt gcccaggatg gaagtagatt acgagggagt
      421 gaatcatgaa aagcttcgct attagtttgt cgcttctgct tttgggtgtt accaaccttc
      481 aggctcaaac tcctttttat gaagggaaaa cgatcagaat cgtcgtcggg cttcccgccg
      541 gtgacgtcta tgatctctat gctcgcatgt tggccgagca catgggtagg catattcccg
      601 gcaaccccaa catcattgtg caaaacatgg ccggcgcatc gagcatgatc acggcgaatt
      661 acgtctataa cgtcgccaag ccggacggct taacgtttgg gtcaattcta ccttcgcttt
      721 atttcgatca gctggttgga cgaccggaag taaagtttga ctggcccaaa ttcacctggt
      781 tggggagttt cgagaagtca aacaacttgc tctatatgcg cagtgatacg ccgttcaaaa
      841 cgattcacga cgtgaccaag gccaatgaat cccctaaatg cggttccacc ggcaccggct
      901 cgccatccta ctacctggta aagctgatga atgaaagcat tggcgcgaag ttcgaaatag
      961 ttaccggata taaaggcgga caggaaatcg atctggcggt ggaaaaaggc gaagtggatt
     1021 gccgcgcttt taccgtgacg acgtactttg cccgcgagcc tttcatcagc tggcgtaaga
     1081 agaattttgt gcgggtttta tttcagacgg gcaagacccg ggacacgcgc ttgcccgagg
     1141 tgcccaccgt tcaagagctc attgaacaat acaagactcc ggaagggacc aaacgcatgg
     1201 cagctttggt attagcatcc ggagaatttg gccgtcccat catagctaca cccggcatac
     1261 cggcagatcg tgtgaagatg ctccgagaag cgtttagcaa gactcttggc gatccagcgt
     1321 tgttggcaga tgcccaaaag agaaggctgg atatagatcc gaccagcgct gaggatctcg
     1381 aagctctggc caaggatgtt ttcaccgcca atcgtgagtt gattgagcga atgaaatcac
     1441 tgctcagtaa gtgacgaagc gtaggctagc ccgagatggc cgtgttcgtg gtagaggaat
     1501 ctattgtgca gaaacacaga aggttcgcgg atgatcctct tgtttcttcg aacgccctgt
     1561 aggtatcacc cgagcaggat cacggatgct taagccctgg gccgatttta cgtcttcagg
     1621 attattttcc cgcgcctgtg gggagcgcct tcgagcatct cgtgggcatg ccgtgcttcg
     1681 tcgagccaga gcacttcgcc gacttgagtc tttagcctgc cggcatcaat gagctcggca
     1741 attctttgca accgatcggc agtgacctca accaggaaga atgacgctcg caccccatgc
     1801 tgctgggctt tttcctggga aggcacagcc gatgaagaga cgatgacgcc gccatgtttc
     1861 acgacgccat aagatcgttc gaggatatcg ccgccgacgg tatcgatgac ggcgtcgagg
     1921 tctttcacct catcctcgaa gcggctggcc cggtaatcga tcacgccgag cccgcccaga
     1981 ctgcggatgt aggaaacgtc attggccgct gcggtggtta tcaccatggc acccgcttgt
     2041 ttggcgaatt gcactgcata ggcgccaaca ttaccggcgc cgccgtggat gagcacagat
     2101 tgtcccgcgc gcagttgggc aaaatcaaag accatctgcc atgcggtaac gctgacaacc
     2161 ggcactgacg cagcatggct gtaattgagt gctcgcggct tgggcgcgat catgctcggt
     2221 ttagcgcgag catattccgc acaagctcca gtgaaacgtt ggttcgtgac gccaaagact
     2281 tcgtcgccaa ccttgagccg cgctacaccg gcgccgacgg agtctatcac accggaaaag
     2341 tcagacccca gagttaacgg gagcgtttgc ggtagaacgc tcttgccgcg gcggatcaat
     2401 gcatcccatg gcccaacgcc ggcggcgtcc actttaacaa ccacctcgcc cgcgtttggc
     2461 acaggcttgg cgatatcttc tgtgcggatt acatcgagac caccaaatct gtgaattcga
     2521 acagccttca tttcaacgtt cagatcgaat atgcccgccg agtccgtcgt ccgaggagcg
     2581 gcgatgacgc gaaccgactc tcggtataca gaccctccgg cgaggtgaat gctagcgcac
     2641 ataaaaatgc atgatcagct gaactgcaag agtgaacgga aatcaactcg ccacaatatt
     2701 gaaatttgtg actgaagctc aggagtgtcg agattagctg gagcgacaac gcggtggaaa
     2761 tactgctgcg aacgtcaatt ctgttgaggt tcgacgcttg ttgtgctgct tactgctgca
     2821 agctgttttc tttccgcggc cggaataaga tgtcgttcca tgagatcgta gatagcgccc
     2881 tggtgctccc gtcgcccttc gccaggcgcc ggcatcgcgg tcgtaactac caccagcttg
     2941 agaccaggca cggtgaagat aaattggccg ccatgtcccc aggcataatg gatcgtctgc
     3001 ccgcccaaag tatcgatcca ccagccgtat ccgtagtcgc gaccgctcca gctcgactga
     3061 gtgcgcggct tgagagattc ttcgacccaa ttcgccgaga cgatctgttt ttcctgcagc
     3121 cggccacggt tgaggtagag ctcgccgtat cggagcatgg ctcgcggagt caaatgcatg
     3181 tcgttgccgc ccaggtaaat cccttgcggg tcgcggatcc aagggcggat cgcaatgcca
     3241 agcggttccg ctaagtagcg gcgagcaaac tcgaacgtgc tcatctttgt ggccttggtc
     3301 agtatcgccg agagcaggtg agtgttgccg gtgctgtaaa tcatcctacc gcccggttca
     3361 tcgatcatgg ggcgggtcaa aacatgacct acccagttgc cgctggttac ccatcgtcca
     3421 tagttgacgt tgctggtggt ctctaggccc gagcgcatcg tcaagagatc ttctgtcgta
     3481 atggtcttct ttacaggatc cgcggtggtg ccgagatgct ccgggaaaaa tttggcaatg
     3541 gggtctttga tagatttgag ctgtccccgg tccagcgcga tgccgatcag agtcgatata
     3601 atactcttgg aagccgattt cagattagcc gggtcggtag gacgcgcgcc atggaaatag
     3661 cgctctccga taagctctcc gtctatcgac atgagcaaac tcctgaggcg gggcagagaa
     3721 tttagggctt cctcgaacga aagctccgtc ttttcattgg tggatgacgc gccagtcgcc
     3781 ggcatcgtca taaccgccat cacggtaaag agaagtgcca gaaggttctt cgtacaacca
     3841 attttcagcc taatttgcat gattctgtcg tatctccaac tttgcgaggc gcggcgcaaa
     3901 acgttgagtg ttttgcgcga gatgaagatc acccaatcgc gtaggaataa taactgaatc
     3961 ggttagcgac agtaagagag atccttgcct gctcgccgat tgacaggaaa gcagcgggtc
     4021 ggttagtctc ccttccaaaa acaaaaatcg ctgcttccta tctcgtttag tattctcgtg
     4081 cattttttgg agtaagttgc tgagaacatt tttcgccggc tgccgcaact cggaccgcaa
     4141 tttcctatcg atgacttcgc gtggaattga tagcccgcag aattgccgtt ggttagcaag
     4201 gctggctttg tttgtgagtt tttttatgct tgctccgacg tcgttcgcga ttgcggcctc
     4261 ggcgattcgg gaattccgct gggccgccga ccctgagggc ggcgcgcctt ttgtcgaagc
     4321 ggatccggcg cgtcctgatc gagtcgtagg attcgacgtc gagatcgccg aaatcatcgc
     4381 gcgcggcctt gaccgctcgg ccactttcat gaacatcgcc tttacctcca tcgatcagtc
     4441 aattgagcgc ggcgacgctg acatcggtct tggagggatc gaagatacgc ccgcgcgccg
     4501 ccgtacaatg gcgccgacgg tgccctatta tagattccgt gaagtgttga gcgtacgcga
     4561 tgcggacgct tcacgctttc ggacactcgc cgatttgcgc gggcgacgtg tcggcacgct
     4621 gggagggacg atcgcctatg agattcttct ccgcgccgag cgcgacttcg acatcaaagc
     4681 cgtgtcctat gatgacgatg tgcatcccta tacggatttg atcatcggac gtgtggatgc
     4741 ggtgctgctc gataatgttc ttgccgagcg tcgccgccgg actacttcgg ggttcactat
     4801 tcagccggaa agcgttgcga ccggccacta cgtcggcgtc cttgctgctc ggaacgctgc
     4861 gttgcgcgaa tcggtcaacg aagttctacg caatgccatg cgcgacggca ccctagagcg
     4921 gatctttcgt aaatggcggg tatggaacga ggatcaaccg gcgttattca ccaacgtgct
     4981 cgcaggcaag cctctggctc cggtagttgg ttttgacccg atcgatggcg atgccatgct
     5041 ttccaaatgg cggacgatgc gcgcttacct gcccgcgctg tttcgcgccg ccgcgatcac
     5101 gatggtgctt tcatgtttgt cgatggcgct ggccgtcatc atcggcgtgt cgattgcgag
     5161 tggacgcatt tacggcggca aaatcgcgcg ctcgcttttg accgcttatg tcgaaatcat
     5221 ccgcggaacg ccgattcttc ttcaactttt tatcttgtac tacggtatcg tcgaactcat
     5281 tcgcctgccc gccttcatcg cggcacttct cggactcggt ctcaactatg ccgcctatga
     5341 aagtgaaatc taccgcggcg cgctcgaagc cgtacccggc ggtcaacttg atgctgcacg
     5401 gattctcggc ttcaatgagc ttcaggtgct aacattggtg cgcggaccgc aggcgtttcg
     5461 tttggcgttg gcgccgatga ccaatgattt cgttgccctg ctgaaggatt cttcactggt
     5521 ttcggtgctc acggtgctcg agctaaccaa gcaaacgcaa atatttgcga ccaacatcgg
     5581 cagctgggtt gttcccggag cattgtgcgc ggcgctctat atggcgatgt ctttgccgct
     5641 ggcggcattg gcgcgccgcc ttgagcaacg atggcgcagg cacacggcat gatcgcaaac
     5701 gtgctgcagc tcaatgacgt gcgccttcgt cgcgctgagc gcgaggtgct ggccggtgtc
     5761 acgttcagcg tgccgcgggg ggaaatcgtc gccttgatgg gaccttcagg ttccggcaaa
     5821 acttcggtgc tcagattgat tgccgggctg gagccgttcc aatcgggcag cgtgcagatt
     5881 gaagatctgg cgatgtttgc caacggcggc actcccgcga acactgtgcg tgcgttgcgt
     5941 cgtaaaatcg gtatggtgtt ccagtttcac cacctgttcg agcatctgcc ggcgatcaaa
     6001 aatgtctggc tcgcgccggt acatgcgcat ggtgtgccgt tccacgaagc cgttcgccgc
     6061 gcgcaggagc tgctcgcgct gctcggcgtc gaacatcgcg ccagcgcgct gccggccgag
     6121 ctgtcgggtg gcgaggcgca acgggtcgca atcgcgcgcg ctctcgctgt agatccgccg
     6181 ctgctgctga tggatgaacc gacagcatct ctggatggag cgcgccgcag cgagttgaga
     6241 gaattgcttc aaagattggt gcgtcaagga cggactttgg ttctcgccac tcacgatgag
     6301 gaattcgcga gcgtctgcgc cacgcggatt ttgcgcatga acaacggtag ggtttctagt
     6361 caggattgaa ctagatagtg ctggcacgcc gcttttgcca ttacgaacaa tcgagttgtg
     6421 ctgagatgcg gcaaatgtca ccgcaagcga tcatgccgaa atcgctcctg aatggttgta
     6481 atgcggcggg tccactgggg tcggcccgcg ctctggtcag cggctgccgt ttagtatgat
     6541 tctagcgagt aagcctcaac acccggcagc tcatcgaatt cagattcatg ttactctcct
     6601 ttccctctcc gctgtcctgg gtttgacggt gctggcacgc ttgcgcaggt ttcgttcgat
     6661 gagccggaga tgtcgccttg gcgaaaggcg cgtaccgtcc agcaaaatgt atcgccggtg
     6721 gctccgggtg gatcatccgt aaagctcgtc acgttgggac cgacgaagcc gacttcatga
     6781 aaaaccccgg acatctcacg ataaatgacc gtgccgtcct cgtccgcgtt catgtcggtc
     6841 caggtgaggt gaaacggatg gctaaacgcc gccataggaa ataataatag cccgactact
     6901 atcgacgtcc ttttaccgat gaatgtcctt aaggttgaag cgccaggcgc attcgacgat
     6961 tgtctggcgt atgtcatccc gattcgattg atattcttgt tagtcatagc tgctcctgga
     7021 tgttgcggct caccagtttg atttagactg agacacaaac acaagctacg tgggagtgtc
     7081 gcagtcgcgc gctgatgagc ggagttgtca cctggttaat ttttgttcgg gcgataaaga
     7141 cgtgcccgta ctccaatctt tcagaatacg gttaatcaat tacacgaatg cttgactgaa
     7201 ttctgtggag ctgaagtgga tcagcaccga ttcgcattgc gtcttctgat gcggaaaaat
     7261 ggagccgctg tatggaatct tcttgcgcag tttggatttc cacccagaac aagtcagtct
     7321 tgcgatgtcg cgtagccgtg gcacgcaagt gaggtgccat aaaccgccat agaaggctta
     7381 gatcctgtgg cgcgcgctgg tttacctatg gcgattcaag catctgggtc gtgagatgac
     7441 ggtttagttc aggatggatg gtgcgaagtc gaaggagttc tcctccagtg ctggcgtagt
     7501 ttatttcaaa cgagcatctt tgcggctttc ggtttgaagt aaacggcggt tgtagaatgg
     7561 aagtcttttt ggtgctcagg aaattctaac cgtcacgtgc ctgcggtcct atcgtagtta
     7621 tctaataagc agccgtagct tcttgcagcg attgtgctgc tcaaaacttt taccgtcgac
     7681 gaagccgtgt attcaatcga tagttcgccg gccgtttgct cataactgtc ataaacctct
     7741 tcggcactgg cgtatctgct cggtttagga gcgagatcgg aaaaagattc gttatcaaac
     7801 cgggtccgac actcggaccc atatttcgtc attatcgatc tttacggagt aggttttcag
     7861 tggaatctcg gccggtaatc tcgttacttc gccggttttg atgcggaacc ttgcgccgtg
     7921 acagatgcat tccacttgat cgttgtcgac gatcttgccg aaatcttcga gtgaacagtc
     7981 gctatgtgag cacatctcgc tggtggcata aacttcaccg ccaactttgt aaagcgcaat
     8041 gcgctcgccg ttgatcctga cggccagaat cgatccttca tcgagttccg agtttgcgat
     8101 agctttattg aagtcaggca ttctttcctc gtattgcctc agcccgcggc aaaaactttg
     8161 tcctgccagc gatgaggcca aacaggatac acggcggatg tgaatttctt gccggatctc
     8221 tttcgaagag agtaaaaagt tgggccgtta gcctgtgctg gtaacttccc accagttccg
     8281 attcaggtcc gagaaaataa atcgagcact cccgttcgca ttttcaatgg catcgactgt
     8341 agtcagtccc agcgtctggg cggagctcga aaaccataga tgcgcctctt cggtttgaga
     8401 tgctgattca agcgcaagaa caaaacgttg cgctcgcgtg ggcagaatcg gctcggcgcg
     8461 aaaaggcaga ttgacaatat accaaggact ttccggatgc ttcacataga tcgaggaggg
     8521 ccagagttgt acaacctcca aaccgagaac gttctcatag aacctacggg tggcttctat
     8581 gtcgtgaaac tggaccgtgc cgtgtgtcag cgcctgggga acgtagccgc gtcctggaaa
     8641 cttctcttgg ttgatgggag tttgccagtg tgggctcgta gttttaccca taccggcttc
     8701 atcggctttc tcgtaagact ctatttccca gaaattccct ccgggttcaa aaaaatggac
     8761 cgagtacgcg ttatggtttt ctctgggttg aatgatttta atcttgtatt ctctcttttt
     8821 gctttgcagg tattggtgcg cttcatcgac ctcacggtta gtcgcgacac gcacgccata
     8881 atgatgttgg aagggcttat ccggagcatc ggcacctgac tcatgcgcga tcagcttcca
     8941 gtccgtattg gggtgcttga gcacggtctc actatcggtt cgtgctacca cttcaagagc
     9001 gagaaggtcc tgcagaagag gcagggtttg ttgcagcgaa cggcattcat aatggcctgc
     9061 aataagccct ttcggtttta acatgatctt ttctcctttc tgcattggca gaaaacggtt
     9121 tctgcttggc acgtaacata attatgagag agatcgcaac gacgcacgac gcggtgtgag
     9181 tcccggccaa tccgctaacc tccaagatca atgcggcgag ttgatttgac ttgacgggcg
     9241 atgggttcct aaatattctg caaccgcttt gcaacggatt cgattctcac agggattcac
     9301 gtgtgaaagc tgagcgctga tgacggcccg ttggtcatga gcgaaggcgc ggtgcgccag
     9361 agctcagaag ttttcattgg ggaactttcg ctggcgctgg tgactgtgct ttctcacgga
     9421 ttttgaaaca cgttccgttt gaccgaagag gcgagcagaa atctatagcc ccagttttaa
     9481 aatgcagccc aaaattcact catatttaga tgcgaggatc tttatgaacc accttcgact
     9541 cacccgtgtg atgatgcttt ttctattgct ggctacgttg accgactacg cggaagcgca
     9601 atcgggcaag accagcagcg cgggtttcga tgagaaagcc gtggcagatt tctaccgggg
     9661 caaaaccatc agaatcgtca tcggttcggg gcctggcgga acctatgata tctattcgcg
     9721 tctaatctcg cggcacatgt ctcgatttat tccaggcaat cctactctgt tggttcagcc
     9781 cagggcggga gccggcggtc ttatagcggc aaatgcggtt tacaatgtcg agcccaagga
     9841 cggcaccgtg atcggctcgt ttggtgaaac ctttcctttg cggcaggcta ttggcgcgcc
     9901 cggcattgag ttcgattcgg caaggtacca atggatcggc agcgccgtga atactgcgat
     9961 tgcctgcgtt ggacgaaccg attccgcggt atcgtccttt aacgatgtta cagccggcaa
    10021 gccgttcacc gtcggcacaa tggctcccgg cagcaccatc tatgataccc cggcgatcat
    10081 taatgcggct tttggaacac agatgaagct ggtgcgggga ttcgatggag ttgccaacat
    10141 cgtcaatgcc gccgagtcca aggaagtaga tggttattgc gcaagttggc tggcaatgct
    10201 gaccaccggc cggcaccttc agttgatcca cgacgggaca ctcaagccgc ttctaatcat
    10261 gggcgacaaa acgccggatc atgcgcttat gaaaggcgtt cctgccgctg agacaatagc
    10321 taaaaccgag gaagcgaagt tgttgctgcg cgcgatgcac gcaccttcgg aaattaccaa
    10381 cccgtacgtg gtccccgccg aggttcccaa agaccgggtt gaagccttac gcaaggcgtt
    10441 ctcagcaact ttcgcggatc cggaatttag ctcggactca aagaagagca aaattgaatt
    10501 ttcgccgagc aacggcgaac gcgtcaccga agtcgttcag tccattctca aaaccccgcc
    10561 cgccatactc gctaaaatga agaaaatatt ggtgcagtga gcgagttgat ctatcgaaga
    10621 gcaaaggatc gctgacggtc tcaaattgac gctgtgttac gcagcaagga ggtccccgca
    10681 aacaaatcct cgattgcact ccgatgctga aatcttgatg cgcgccgggg atcgcggcag
    10741 cagggtcgac aaccgccaat gccgcacata ccaggaaatc gcaaaccatg gcgccgcttg
    10801 cttctacttg ccggaaagag tttccgcgct aaggatcgac ggttcaccct aaaacttaag
    10861 cattgtgatg aatgccagga tcaattattt gctgcttact acagctttgc tcggaacctt
    10921 cttc
//
LOCUS       DASUVS010000447        34678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1730192, whole genome shotgun sequence.
ACCESSION   DASUVS010000447 DASUVS010000000
VERSION     DASUVS010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 34678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34678
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730192"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VF182_23415"
     CDS             <1..466
                     /locus_tag="VF182_23415"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEX6440085.1"
                     /translation="GVTGSGKTTIGKLLAAEMHWKFYEGDDFHPPANIEKLRRGDPLD
                     DQDRLPWLTAIRDTIRAAIDRGEDAVVACSALKESYRRMLRISPDVIMVHLEADPELI
                     EKRLKHRSGHFMNPVLVQSQFETLEEPEGALQIYAGLAPAEIVGAIRSRLSL"
     gene            605..979
                     /locus_tag="VF182_23420"
     CDS             605..979
                     /locus_tag="VF182_23420"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX6440086.1"
                     /translation="MPYSFLLSVPGRRTGEMRSVPVNLLQRNGKLFLVATRGNTQWAR
                     NTRASRKIFLTRGSLRMEFFVRLIPNSEKPAVLQSYLDRFKWMSWRFFPLAPNSPPAM
                     FEPIAARYPVFELIRVPPEIHS"
     gene            1061..1429
                     /locus_tag="VF182_23425"
     CDS             1061..1429
                     /locus_tag="VF182_23425"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX6440087.1"
                     /translation="MAALVLLVLGHYRMISFSGALICFGAWMLKDLLFYPWLRSTYEV
                     STRTGSKTLIGYKGVAESPLAPEGLVRVRGELWRAVAIPRDLVIRTGIAVEIIDANGM
                     TVFVRPLSDDSAINDLTENE"
     gene            1573..2667
                     /locus_tag="VF182_23430"
     CDS             1573..2667
                     /locus_tag="VF182_23430"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6440088.1"
                     /translation="MSNEEIESFLSIEKCISALEKAYASWDEGKAINRPRTDLVMPSS
                     NGTGVYAFKSMEAGLYDPPIVAMRINSDIIRWQQQGSRVIKAKVPAAPGDKYVGLVML
                     FSTLNGEPLAIFPDGVAQRMRVASSSALAARYLARQDASTLAIFGSGWQAGSHLPAMC
                     AVRNITRVNVFSPTKANREAFAKEMAQKVSAEVRPVESAEEAVRDADIIAATTNSLTR
                     VIEPEWVKPGIHLTCVRVPELGDETIHRMDRLVIHARQHAPENYIAGFGDQAIESHDA
                     IDIIRKGPAQAREVKVEHPFWLTSPELRDLIIGRAQGRESAQESTCFLNNIGIGLQFA
                     AVGAAILAEAKNKGAGHEIPTDWFLESVHP"
     gene            2831..3784
                     /locus_tag="VF182_23435"
     CDS             2831..3784
                     /locus_tag="VF182_23435"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440089.1"
                     /translation="MAIFSFLVSPAMAQEKLRFYLGASSKTLGYSPLWVAGKKGFFEQ
                     QGLDVQLLLLRGTPMTVQALAGASLNVGSGSPEAFIEASERGLDLVIIGGVINGLTHY
                     IVGGKNYKTYQDLRGATLGASSLTSGTVTAFKQALKVKGLEYPRDYKILVVAGGSAAN
                     LAALQAGQIAATTVAIPLNYLAEEAGFNVIGRLIDAVPDFQLTVLAAKRSWVEKNRAS
                     MVRFMKGMAQANRWLYDNKEPAIEFLTREMQLKPNHAARGWEYYTQNRIWHPDGDLNL
                     EGMKYNLRIYEEQTGAKGPSDYAKYIDQSYLYDALKELKKK"
     gene            complement(3857..4429)
                     /locus_tag="VF182_23440"
     CDS             complement(3857..4429)
                     /locus_tag="VF182_23440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440090.1"
                     /translation="MKRLSKMILAGLTGVALMLPALPAPADAKDDDGYKHSRRDGDYR
                     RFDGPHGRSDAHRFNRRGNYRRGETRHARFDDSHFRRDRHPGKYQGNRGHHNKPVVRE
                     KFSNVRDARKDVQEGRRELRGDFKELRKDRAELRRDIRNGASKEEIFRDRQEIRDDYK
                     EIATDRRDLRQDQAELRDARGELKDALHKR"
     gene            complement(4515..4877)
                     /locus_tag="VF182_23445"
     CDS             complement(4515..4877)
                     /locus_tag="VF182_23445"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEX6440091.1"
                     /translation="MKQNLNQLRSFRWVIAVSFAALTAVGCAGGPLTTREKGAGIGAV
                     GGAAAGGLIGAAAGRPGLGAAIGGGVGLGAGALVGDYMQGQEEAQYQYNQQQIQQNQR
                     VIERNRNEVQTQRQRQEY"
     gene            complement(5453..6469)
                     /locus_tag="VF182_23450"
     CDS             complement(5453..6469)
                     /locus_tag="VF182_23450"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440092.1"
                     /translation="MSVTGRVASLLGAFLCISTMYLAMAAYAQENFRVSYGGYNETAA
                     PMWVGIDRGFFKKYGIDGSMIQVRSGSLSIAALVSREVEAVWPAQSTILSTVAGGVKL
                     GCIASATNKIPRQLVARKEIKTIEELRGRIIGVQSIGGGFWLQTMIILDHLGVEPDKY
                     GLKMRVIGDGPVIAQALLNGNIDAAALTYSLSEAPLKAGFRSLADAAELKAPYQGPSI
                     CALKDLIANRQDFFLRVTKGLVEATAYILDASNKPEVMKTLQKHLRLQKTDEAEASYK
                     VLRLMATLDIAPNPAAYKSVQRIVATVNPKIAQVDIEQIMDSSFARSLESSGFLAEQR
                     KKVR"
     gene            complement(6964..8868)
                     /locus_tag="VF182_23455"
     CDS             complement(6964..8868)
                     /locus_tag="VF182_23455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEX6440093.1"
                     /translation="MSSILSIAFFFLCFLPNTAGAQDSGNTYLEQLLQRAKAMALHEQ
                     RYWHLLLHYRSSLGGGVESEADEPGFFLAPDGKTDPEAELQATLTRFFSTDLVGRSPQ
                     PAHCAFIARYHWLKSTLAIDETRMPQQPCEQFQSWITELNPASITVIFPSAFMNNPSS
                     MFGHLLLRVDQKGQTERTSILAYTINYAADVTTDNGILFAVLGLTGGFKGYFSTHPYY
                     IKAKEYGDFENRDIWEYRLDLTEDQMQRLLMHAWEMGSATFDYFYFKENCAYQILALL
                     DVADPELQLAERFWFYTFPSDAIRMIADKPGLVNDVTFRASRSTRIRRGREALSAEER
                     NWLSKMVTDPKLSQSDSFTHLPPERQAAVLDVVSDYLLYKSATAENGGAFESRNRTIL
                     LARSKLKARPVPLDIAPASGAPDQGHKTMRAGLGFGWREGEFFNEANFRLAFHDLLDP
                     EYGYTPGAQIQALGLTLRHYARSDHTRLEKFTLLDILSLSPMDALFASPSWKINAALN
                     TIQHHNCRFCRTGNLNAGIGVARESAWLNREVYFGLAEFATEVGPVFHGNHRVGGGLT
                     VGALSDITDRWKFALSGGYLNFPLGEKSDEWRLSAQQRYTLTRNFTLRFDFNQRARTQ
                     ELVLNLQAFF"
     gene            complement(8913..9422)
                     /locus_tag="VF182_23460"
     CDS             complement(8913..9422)
                     /locus_tag="VF182_23460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HEX6440094.1"
                     /translation="MIKKQWLRLGVALLPLYAAQVWGAPNPDTGPGCGLGKLAWGEYG
                     NQKDIAPQVMQSTTNGTFGSQTFGISFGTSGCTNNGVIMSEHKTSVFAALNFENLSQD
                     MAKGDGEHLSSLAELMNIPQENRAEFYALAQLQYQSMIQSGESTPAALLKALTSAMSS
                     HPLLASSLK"
     gene            complement(9581..10639)
                     /locus_tag="VF182_23465"
     CDS             complement(9581..10639)
                     /locus_tag="VF182_23465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440095.1"
                     /translation="MITRNKFIRIASFYGLVFCICSNDALSQSNFYEGKTVTLIATTS
                     AGGTGDLRVKAMAPFLKKHIPGNPTVVIQYIDGGGGRKGTNSLYSNVRPDGLTIGAAS
                     GAIIGLAVMGEAGITYDIDKFIYLGSPESENHYIIYTRKELGLDNLEKLASKPGLRIG
                     AQTVGHVSYVAGRLFAYFLNLKDPRFIAGYTAHELDVALTQGELDSRANNASSVIRRN
                     SDWVEKGLMNFHAIMEVPKGDKHPRLGHLPEIGSFAKNDQENKLLTLWRAFRSVGSPY
                     ILPPGTPKERVAILEEAMHKIFKDPEFPPYFKKLVTDDASPMGAAELRKVVAEMPRDA
                     ETINMLKKFAGPGPLPPR"
     gene            10854..11534
                     /locus_tag="VF182_23470"
     CDS             10854..11534
                     /locus_tag="VF182_23470"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX6440096.1"
                     /translation="MKAMTMVVSADGGRALRGDIMQIELNYPDPVPIELDPSKTLLLV
                     VDMENEDAHPSGKRFAGQAVTRIIPNIARLRRKVRQSGGSVVHTQSVRKPDALEFTVF
                     NNAIRKIEGTWGAEFVDELRPEADEPIVVKYTHDCFYRTEMESVLEGFNLRPGDARII
                     VTGISSRSCVQCAVTGFSIRSYYVYVPIDCTGQREEEEKLQAFSLFASFAYRYNVTVT
                     RSDLIQFS"
     gene            11579..12862
                     /locus_tag="VF182_23475"
     CDS             11579..12862
                     /locus_tag="VF182_23475"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HEX6440097.1"
                     /translation="MGMTITEKICARAAAKDRVGAGDMIDAAIDKLYIKDLRFSKAED
                     PKGLYGIFKEILKDMGITKAWDPDKIIINFDEQPARSTRRAEGQERAKQFAGEHGATV
                     YEGYQGGIGHNVMVEKGHVVPGEFIVGADSHSCTYGALACFATGIGFTETVGVLATGR
                     IWLKVPPAIFIELTGKRPPWIWGKDIALRIMGAMGANGAVNKTIEYGGRAVAQISIES
                     RLSMCNMAVESLALNAVFPPDESAIDYVKQVSDASFAAVESDADAEYESRLRFDLTDM
                     EPFVAAPGVPSNGQPVHEFVGTKIDQAFIGTCSNSRIEDLRVAAKILKGRTVHPGVRM
                     IVTPASYAAYFQAREEGLLQIFEKSKVLLTASECGICTRPSLASGEVCISSGNRNFPG
                     RMGHRDAQIFLGSPATVAASAIAGEIVDPRELLPS"
     gene            13061..13543
                     /locus_tag="VF182_23480"
     CDS             13061..13543
                     /locus_tag="VF182_23480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HEX6440098.1"
                     /translation="MKRIFLGKVWKFGDCIDSGNIKGVMAGIDPQFHLKVKPGDFIIA
                     GINFGMGSSAEDAPRSLREAGIAAVLAESISNIYLRTLINLGLPAIECPGISTLVAQN
                     DEIEVDLRSGTVCNLTNGASLAFPPFSDHAIAILERGGLIPYLKSSFAKQDKSAPLNS
                     "
     gene            13552..14448
                     /locus_tag="VF182_23485"
     CDS             13552..14448
                     /locus_tag="VF182_23485"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HEX6440099.1"
                     /translation="MKATTKLRELLKGDDMIITPGVYDCLTARLAETTGFTAVKLLGN
                     VTCASLLGLPDLGLIGLNEMANHAKNVAAAVDIPVMVDADTGYSGGPLGIARTIKEFE
                     RAGIAGIGIEDQVTPKKCALIPGGTPVVSIKEQVRKLQAAVEAKEDSDFVISARTDAE
                     SVNGLDDALDRIKAYEEVGIDMVGVAMSWVRKEGMRQRTLDALKRIRDSVKVPVNCMF
                     MDEAIQVGNVTLAELKQIGFNMGGSNAVRYTVIKAVTEMLTILKNEGSTKNYAHRLAS
                     LKEYEALVRLPEFLEMELRYSI"
     gene            complement(14552..14989)
                     /locus_tag="VF182_23490"
     CDS             complement(14552..14989)
                     /locus_tag="VF182_23490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEX6440100.1"
                     /translation="MLEFSDVTVAHAIQLAVAPVFLLSGIGAMLAVMTNRLGRIIDRA
                     RYLEEQLVDVSPEFFSMLQTDLRLLSQRAKLIYSAIALCTTTALLVCAVIAFLFVSAF
                     LRFDASVVVALLFIAAMSTFVLGLLWFLREIYVGTISLRIGPR"
     gene            complement(15037..16020)
                     /locus_tag="VF182_23495"
     CDS             complement(15037..16020)
                     /locus_tag="VF182_23495"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6440101.1"
                     /translation="MYNGQTVVDVHGHMTTPAGFRQFLAEIVSQNTPGRRLEVTDEQL
                     ENAQQRHLKFMDDRNVDVQLIGPRPIAMWHWMRPFLQEFWSENTNNVIARIVKLHPDR
                     FRGMAQLPQCSTSKDTRNCLPEFERCVKELGFVGAYLNPDPGGDKQTPGVHEEYWFPL
                     YEKAVELDVPLMIHPSVTYDRRLEIIPANYQMNNYIEEFVAMQLLTHSKVFEIFPKLK
                     ITICHCGGGLNRFIPTDKHVGQGDFSNNLFYDSCVYDVHYLEAAIKQRGIDQILFGTE
                     SPGSGGAIRPETGMPSDDLIPVIDGLGFLSAEDKLKIFQKNPLKVFTKVKI"
     gene            complement(16253..16699)
                     /locus_tag="VF182_23500"
     CDS             complement(16253..16699)
                     /locus_tag="VF182_23500"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6440102.1"
                     /translation="MSGIRRILYATDYSKASGRAREKAVELAKDYDAELWVVHVIAPT
                     TAYLSEQEFGGAELYMRLEESAKQQAESSMKRLMATLEKAKIKAKSLLLRGSPHEQII
                     KAAKNKKADLIVIGTHGRTGLSKLFMGSVAGRVVSTATCPVLTVRQ"
     gene            complement(16729..17832)
                     /locus_tag="VF182_23505"
     CDS             complement(16729..17832)
                     /locus_tag="VF182_23505"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6440103.1"
                     /translation="MAIGNGHGQGINGVIISSDSHVMEPVDLWKKGVPEKYREDAPLF
                     PPHKVGEGFQQHAGGWDPRARIQEMEKDGLSGEVLYPTLMLDLFALDDPGMQEACFRV
                     YNDWLIDYCSVDTNRLIGIPAISVYNIENGIKELERCRNAGLKGAIIWQAPHPDLPFH
                     SNHYERFWAAAQDLETPVSLHILTGHSYNKDKNRNGVERYRGSVNLKLMDIANALFEL
                     IFYGVLERYPRLKMVSVENEAGWLPWITQQWDYYYRRFRKANPPPFSKDPSEYFRNQI
                     YSCFFNDSVCGHNLEWWGEDNIMWSNDFPHPNSTWPNSLKIIQRDLGHLPVEVQTKVL
                     ATNVCKLYNLDLSKVPAGIAKTALAAQAVQASA"
     gene            complement(17910..18584)
                     /locus_tag="VF182_23510"
     CDS             complement(17910..18584)
                     /locus_tag="VF182_23510"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007972914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6440104.1"
                     /translation="MALKRAGTKNELTKISSITLDHYNQHADSFWEGTRDHDVAQNRA
                     ALLEHMRGEGSFRILDFGCGPGRDLKAFKDLGHEVIGLDGAQRFVEIARDFSGCEVWR
                     QDFLRLKLPENFFDGIFANASLFHIPSQELPRVLKELWQTLKAQGVLFSSNPRGDNQE
                     GWNGKRYGAYYNLEQWRKFMTAAGFTEITHYYRPPGAPRAEQPWLASLWRKPETNSAI
                     VDIDSN"
     gene            complement(18758..19750)
                     /locus_tag="VF182_23515"
     CDS             complement(18758..19750)
                     /locus_tag="VF182_23515"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEX6440105.1"
                     /translation="MSKPETGRARIAILDDFEKISDTVPAFDKLKARADITLLRERLD
                     SSKKIVQTLRPFDIILLMRERTSFTGNEYRQLPNLKFISQTGRTSVHLDLPSATRHGI
                     AVSGTAADTGATTKELTLGLILSLMRKIPQVDRRMRTEDWPAIPGWTLEGKTVGVFGF
                     GRIGKEVARVMRTLNTRVLGYSRTLTPEKAAEVGAECVSFERLIRESDIITIHINSNA
                     QTRGIIGEKEFAMMKRGAFLVNTARGPIVSEPAMISALESGQLGGVGLDVFDLEPLPM
                     DHPLRKFENAVLLSHRGYATVEVLGERYEQAMANILDYLDGRPLKLLNPEVLPK"
     gene            complement(19823..20506)
                     /locus_tag="VF182_23520"
     CDS             complement(19823..20506)
                     /locus_tag="VF182_23520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440106.1"
                     /translation="MRKKFLTWLGKDFIELSGEAKPGASASNEASELFRRFDQELKTH
                     KLSLDNVVRTRLWGRDRESRNLGSDERVKVLSGQARSASSSYIAPDHFDSDAKIAVDL
                     LAMKPSRQNMQKRIVEYDPPIVPIRFLVYDSVVMLSGVTTVLPTLEEQLDNILPRITG
                     SLKDSRSSWERVARISFYLHRSQTIDNLNRLFNERIKASTAQREYCFVDGYSSVGKLC
                     EVEVTATTE"
     gene            complement(20763..22316)
                     /locus_tag="VF182_23525"
     CDS             complement(20763..22316)
                     /locus_tag="VF182_23525"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6440107.1"
                     /translation="MGRLGIVLVMLLIVNLKASDSSAQDQIRDLVVKIHAVHHTPDVL
                     RPWSRNTPQQIKGSGVVIDGNRILTNAHVVRYASQIYVQPNQSSQHLPARVDSITPSM
                     DLAILKLDDETFFEKRGSLAFAKELPRVKDNINVYGYPTGGTELSVTQGIVSRIEYTD
                     YYYQAAGLRIQVDAALNFGNSGGPAVSDGKLVGLVFSLIQNAQNIGYLIPVEEIQLFL
                     DDVKDGVYDGKPQTHDLIQTVENAALRQRLGLPKELSGVMVAQPYRDDDAYPLKEWDV
                     ITHIGGTPIDSDGKVAVRYDLRLSASYLTQKFAKNGVLPLTIYRDGKLAKIDLPVKTQ
                     RELVIPYLLNSSPRYFIFGPFVFSQTTQDYLERLGNQRPSSAGSFGRGASPLVTRRYD
                     RPAFDDEELVMVASPLFPHRITQGYDDPNRGVLSEVNNIRVRNLRHLVEILRDSRDQQ
                     ISFKFASAGVLTHETMVFNRSELIEATGKILEENGIRYPYSADLRTIWEAPEAQAPKS
                     AKMFCCSPG"
     gene            22599..22907
                     /locus_tag="VF182_23530"
     CDS             22599..22907
                     /locus_tag="VF182_23530"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX6440108.1"
                     /translation="MWYLVMRRPVKPREQWTVNLDQHLVWMKQQHDSGRILFSGPTPD
                     RKFGIYVIKAASRAEADRIAGSDPYTAAGFCEYDLYEWDVHQIMGAGPFSAAELRTHR
                     "
     gene            23817..24428
                     /locus_tag="VF182_23535"
     CDS             23817..24428
                     /locus_tag="VF182_23535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440109.1"
                     /translation="MKTASDWLLKQEGRTPGILARLIGAFMTPSNAVALNPDLKGWAW
                     VPRTFSWVEPTSYALIALKQTRTAVGTKILAERIQQGELMIYDRMCNGGGWNYGNSKV
                     FGEALWPYPDVTAVALIALQDHQASQANQESLTALRRMMRETDSGLALCWGTICLSIY
                     GGETSQWKRQIEKRYQDTGFLGEIKTLALSLIASVDDAKAFRV"
     gene            24564..25424
                     /locus_tag="VF182_23540"
     CDS             24564..25424
                     /locus_tag="VF182_23540"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX6440110.1"
                     /translation="MQALRYDLSLREIITKGVRLCGLEVKGKTIVLKPNLVEFDPGGV
                     INTNPLVIEAAIDCFRTLGARQVLVAEGPGHRRDNEYLLAASGIYAVIKEHRVAYVDL
                     NTDDIRPTRLLSSFTKLEQLYLPETLYNADLIVSMPKLKTHHWAGVTLSLKNMFGVVP
                     GSVYGWPKNILHWQGIHNSILDINSSLPVRQFAIVDGIVGMEGNGPLQGQAKESGVLI
                     FGKDLVAVDATAARLMKIEPKKIKYLAQAGEFLGNVNHEKIEQIGENLDRLQKDFQVI
                     EEFQDLKTLT"
     gene            complement(25591..27015)
                     /locus_tag="VF182_23545"
     CDS             complement(25591..27015)
                     /locus_tag="VF182_23545"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440111.1"
                     /translation="MLRLIKQAFTRALTISALCALPFGAVVGDASSAESVATQPLRKV
                     MIGYSAISPSQAPAWIAQDQGFFRKYGLDSQLLFVEGGSRTVQTLISGDVLAAQVAGS
                     SVLQSNLQGSGVVMVAGFLNTMDYKLIVSRDITRPDQLKGKIMAVSRIGSSSDFATRY
                     ALEKYGLTPDKDVAIVQIGSQPARFSALETGKIHGVMIAIPLTAKAKRMGLNTLADLQ
                     MLGLEYQHTGLAFSQDTIKKQPELVRNVLKAFVESIHFMKTRRQETIAILAKYLRSDD
                     TEALDEAYDAVLNSLIPEKPYPTIKGIQIMLREMGVKEPAARSARPEQFVDLTFMKEL
                     DSSGFVDQLYKTQSLAKATSRAKPTPVAVAVNEKPVTIESKIKPAAAESKASEIAKPA
                     TTASEKTPAPAATTAKPVKSGTPADQQYVVKVGDTLSKLAERFYNSTSKWEKIFEANK
                     ESLKNPNYIYVGMKLIIPADDQAS"
     gene            complement(27051..27833)
                     /locus_tag="VF182_23550"
     CDS             complement(27051..27833)
                     /locus_tag="VF182_23550"
                     /inference="COORDINATES: protein motif:HMM:NF028512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX6440112.1"
                     /translation="MSQESLSIETTKVNKPLNIAWQGASLRWKISVAFSGLILMLGVL
                     VIGIVYTMTDSALHRQVDLRAMAIATNLSDASAAYVSRQNTLELDVLIAKYGRLDGVA
                     YAYIQDPKGDIIATSLQPFPSELKDSSAVERRTAHSRVTNIRGKSIYDTRVPLLEGQL
                     GTVHVGLWADSIQTDVRGTVFTIIALIAGCLALGIVLSLLLAAKAIRPLMELKTSADE
                     ISRGQLDTPVTIRSTDEVGELARSLERMRASLKAAMVRLNRE"
     gene            complement(27861..28655)
                     /locus_tag="VF182_23555"
     CDS             complement(27861..28655)
                     /locus_tag="VF182_23555"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX6440113.1"
                     /translation="MQGNLSQISLNDILMLATGGKKSGVLKLSRGKETVEVYLVDGSI
                     IHATCPIGDGEKALLYPVTWGEGNFALLPTGTPPSATIKKSSSEILDEVKAMSREWEN
                     ILEVIPSGKTILRIADLGEEQNGPVTVPHVGWRVLSKIDGNRSVQEIAEALRIPYAYT
                     AKVIFNLQKAGLVEIVAPSSRPSTEMVPASVLGRVIDLLTEIMGPMASLVVRDQIEGL
                     GETQAQFPESKLHDLIEHVGKEIGDAKLRLKFEESAFQEISTLKRF"
     gene            complement(28682..29008)
                     /locus_tag="VF182_23560"
     CDS             complement(28682..29008)
                     /locus_tag="VF182_23560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440114.1"
                     /translation="MKNELQNLTEDISRLYEEITQKTESLGQIDSITRLYDELQTQLL
                     GISTEEVDLLQTQIKSTLEQMVGISKSLGVIKTLKVTLNSNEEVLESVKKAQTSTSGR
                     RTLEDR"
     gene            complement(29029..30189)
                     /locus_tag="VF182_23565"
     CDS             complement(29029..30189)
                     /locus_tag="VF182_23565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440115.1"
                     /translation="MEEIAAAENKSRTLLRGLVNLQSRVGSLVAIKRSMTVVYEAVEK
                     AKSEDINLSTIDALGKPIHLRDIIQASNLIKFPDLKRTLRPRPESTVPIAPSAGPIHQ
                     EAKDTPKSAKEVIRARSAAERRESTESPNEPAFVFDAAEIEAAPGIPSADFSWDKDSD
                     PDQNNIQNLRYEPEIQTTPLGLEPDKTTHRDVSTTEFVQEQVPEESNTSISTALVPIG
                     GDFDQRLLDDLIKNYGEFFSSSDSPKKVDSEARTDTNEIGNKPIFPEKVALESATNNT
                     ARALPLKKEGDIDRELKKIIKDYGQYDIYSQQNRTNVKYAALGVFLLLVALSSGFYFF
                     SSSKSQEAPSVPAAAEPVSATSDESTSGIRDLKRPSKANDGKTPNKSPTLKK"
     gene            complement(30729..32012)
                     /locus_tag="VF182_23570"
     CDS             complement(30729..32012)
                     /locus_tag="VF182_23570"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6440116.1"
                     /translation="MSRQLALLIYSIDSSLSGLAKLLEARDCFIHETRSLEEAEHAFN
                     ALPIEQLKFVFADLTLCRGNTWPRFLDTIYRLGTQVTLVCYHPRNTHSLYTLLGQPSP
                     LTSDSLVPNSIKPPLMIGETPQFYEVLNLANRYAMHDITVLITGETGTGKEVMAQYIH
                     SNGSRATKPFVGCNMTAIPETLVESELFGYVRGAFTGAERNKKGLIEAAEGGTLFLDE
                     IGELAPAIQLKLLRFLETRQYYRVGESTPKLSDVRIVAATNKELEEAIQGTGFRKDLY
                     FRLNSARIILPPLRARKEDIILMVANFVYQACNQFHKPLKTLSSSAKALFLDYPWPGN
                     IRELKSVIESAAMVSDGQYITIADLPMNLQHYATGHRQEIGTKAIKNIEEGERNLIED
                     ALRQTNGNKAKAAEALGISTRTLYRKIERFEILQE"
     gene            complement(32027..33478)
                     /locus_tag="VF182_23575"
     CDS             complement(32027..33478)
                     /locus_tag="VF182_23575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX6440117.1"
                     /translation="MAIGELLRAKGIINDSQLQQVLRQQKIAGGRLGDNLISLGYISR
                     ENLDAILHEPPPIPKTSEQTGLEGNFILNCLLRTMYVSALQTIPELSEHVKLTRGVVE
                     ELLTVAKKDALVEIRGPSEKNHNIMRYALTNAGKERASEALRLCEYIGPIPVPLQTYQ
                     LQVQKQTITNEIVSIDKLKKSMSHLVLSEEIVRRLGPASNSGRAILIYGAAGNGKTAM
                     AEALISAFEQPVYIPYSIEADGQIIKIFDPSVHVPFGADPRDVSSDKHPMFLPHLPYD
                     PRWVRCRRPYIISGGELTLAMLDLDFDVHSKYYEAPLQMKAIGGIFVIDDFGRQRVRP
                     HELLNRWIFPLERKLDYLTLHTGKKFAVLFDQLVIFATNFPPEELMDAAQLRRVHYKI
                     KISPPTADEYKEIFQRVCESYGLEFSEEIIAYLLNSFYIKHKVPFAGFHPKFIAEHII
                     AACNYMGTQPRITRQLLTDSLENMVVLPNPSSS"
     gene            33909..>34678
                     /locus_tag="VF182_23580"
     CDS             33909..>34678
                     /locus_tag="VF182_23580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440118.1"
                     /translation="MGFFTHSAVKTPDERFLEVVLGTGENIDVGEYSGKVARLFSAYG
                     RELLLILASDLEIYNDRELSGYGLNFTWRTAGSRITTERAIVYFPKEKVRAFLKEELG
                     ENTLLAEAVIFVMESEGQANRLSFRAPEPAPDVRAPIQEQVLAPRAIKEKADVEPLVA
                     RGDANSTKTIEPGTNADETSAREKKRPIPSSQMQTSPNPVTPRRNLATKEEIVPDPKA
                     GAFVGDKAEPVGRGPASESATESAGDLMPAVGVIERMET"
BASE COUNT         7936 a         8834 c         9397 g         8511 t
ORIGIN      
        1 gggagtaacc ggctccggca aaactaccat cgggaagctg ttggccgccg agatgcattg
       61 gaagttttac gagggcgatg attttcatcc gccggctaac atagaaaaac taagacgggg
      121 cgatccgctt gatgatcagg atcgtctccc atggctcaca gccatcaggg atacgattcg
      181 tgcagccatc gatcgaggtg aggatgcggt cgtagcttgt tccgccctga aggagtcgta
      241 ccgacgaatg ctccgaatca gtccggatgt gatcatggtg catctagagg cagatcccga
      301 actgatagaa aagcgattaa agcaccgcag cgggcatttc atgaatccgg tattggttca
      361 aagccaattc gagacgctcg aggaacccga gggtgcgctg cagatctatg cgggcttggc
      421 ccccgcagaa atcgtcggtg ccatcagatc tcgcctctcg ttgtaatcta gcgttttcgg
      481 tggttgctgc gtcatccgca agatgcctca aaaagttgga actccgccga gtttcatttc
      541 tccgggaccg attgcccgtg tgctgaatcg attgtacggc tcactaacca gggtcggatt
      601 gagcatgcct tatagtttct tattgtcggt acccggccgg agaaccggcg aaatgcgctc
      661 ggtgcccgtg aatcttttgc aacgcaacgg caagttgttt ttggtcgcga cccgcggaaa
      721 tacgcagtgg gcgcgcaaca cccgtgcgag ccggaagata ttcttgacgc gcggaagcct
      781 acgaatggag ttcttcgtgc gactgatccc caacagcgag aagcctgccg tcttgcaatc
      841 ttatttggat cgattcaaat ggatgtcctg gcgctttttt ccgctggccc ccaattcgcc
      901 gccggcgatg tttgaaccca tcgccgcgcg ttatccagtt ttcgaattga ttagagttcc
      961 cccggaaatt cattcctgag tgttcccact taatgcgtag cctgctcgct ccatgcccac
     1021 ctggcttaga tatctactgt ttcaaattcc cggatggtgc atggcggctt tggtgctgct
     1081 ggtccttgga cactaccgaa tgatttcgtt ttcgggtgcg ctgatttgtt tcggtgcctg
     1141 gatgctcaaa gatttgcttt tctatccatg gctcagaagt acctatgaag tttccacccg
     1201 cacaggatcc aagacgctga tagggtataa aggcgtggcc gaaagtcctc tcgcgccgga
     1261 aggattggtt cgcgttcgcg gcgagctctg gcgggcagtt gccatcccgc gcgacttagt
     1321 cattcgaacg ggaatcgcag tggaaataat cgatgccaac ggtatgacgg tttttgttcg
     1381 gccactcagt gacgattcag ccatcaacga tttgacggag aacgagtgag cggatgctgc
     1441 ctaaggagac gctgctttca atcgccaagt gttcgcccaa gccgcaccac tccatttgcg
     1501 tgcgtctgtt ggcgggagcg tctcttcctt gacctctttc tatcgcgttg atatgtcagc
     1561 aggatgttaa tattgagcaa cgaagagatc gaatcattcc tctcgattga gaagtgcatc
     1621 agcgcgctgg agaaagccta tgcaagttgg gacgaaggca aagctatcaa ccggccgcgc
     1681 accgatctgg tcatgccgtc gtcgaacggg accggggttt acgcttttaa atcgatggaa
     1741 gcgggccttt acgatccgcc gatcgttgcc atgcgcatca actcggatat catccgatgg
     1801 cagcagcagg gcagtcgagt gatcaaagcc aaagtgcccg cggcgcccgg ggacaagtat
     1861 gtagggctgg tgatgctgtt ctccactcta aacggcgagc ccctcgctat ctttccagac
     1921 ggcgtggctc agcggatgcg agtggcttcg agcagtgctt tggcggcgcg ctacctggcg
     1981 cgccaggatg cgagcaccct ggcaattttc ggatccggct ggcaggccgg cagccatttg
     2041 ccggcgatgt gcgcggtgcg aaatatcaca agagtaaacg tcttcagccc gaccaaagcg
     2101 aaccgcgagg cgttcgcgaa agaaatggcc caaaaggtga gcgccgaggt gcggccggtt
     2161 gagagcgccg aggaagcggt tcgtgacgcc gacattattg ccgcgaccac caactccttg
     2221 acccgagtta tcgaacctga atgggtaaag cccggcattc acctgacctg cgtgcgggtt
     2281 cccgagctcg gcgacgagac gattcacagg atggatcgac tcgtgattca tgcgcgccag
     2341 cacgcgccgg agaattacat tgccggattt ggtgatcagg ccattgaatc gcacgacgcc
     2401 atcgacatca ttcgtaaagg gccggcgcaa gctcgcgaag taaaagttga gcatccattc
     2461 tggttaacct cgccggaatt gagagatctc atcattggac gagctcaagg ccgtgaaagt
     2521 gcgcaggagt ccacttgctt tctcaacaat atcggcatcg gcttgcagtt tgccgccgtc
     2581 ggcgccgcga ttttggccga ggcaaagaat aagggcgccg gacatgagat tcccaccgac
     2641 tggtttttgg aaagcgtcca tccttgatgg ttgaaccgca tttgcggcaa cgcagatctg
     2701 acaaattgaa cgcgcaaaag ttgtgataga gaactgtgcc acggtgcatc tgcacggttt
     2761 tcccttgtga taggtttgag aatatcgata aaggtacaaa gtgagggtct tccttagaat
     2821 cgctgttatc atggcgatct tcagcttcct tgtctctccg gcaatggccc aggaaaagct
     2881 gcgcttttat cttggcgcat cgagcaagac cctgggttac agtccgcttt gggtcgcagg
     2941 gaaaaagggg ttcttcgagc agcaaggctt ggacgtgcag cttctcctgc tgcgcggcac
     3001 cccaatgact gtccaagcct tggcgggagc gtccctcaat gtcggttccg gcagtccgga
     3061 agcctttatc gaggcttcgg agcgcggctt ggatctggtg attattggcg gcgtcatcaa
     3121 cggtctgacg cattacatcg ttggtggtaa gaactacaaa acctatcagg acctgcgcgg
     3181 tgccacttta ggcgcctctt cgttgaccag cggtacggtg acggcattta agcaagcgct
     3241 taaggtcaaa ggacttgagt atccgagaga ttataagatc cttgtggttg ccggcggttc
     3301 ggcggccaat ctcgcagcgt tacaagcagg ccagatcgcc gccacgaccg tggcgattcc
     3361 gcttaattat ctcgcagagg aggcaggctt caatgtgatc ggcagactga tcgatgctgt
     3421 gcccgacttc caattgactg tactcgcggc caagcggtca tgggtagaaa agaaccgcgc
     3481 atcaatggtg cgtttcatga aaggcatggc gcaagcgaat cgttggttgt atgacaacaa
     3541 agagccggct atcgaattcc tcacccgcga aatgcaactg aaaccaaacc atgccgcgcg
     3601 cggctgggaa tactataccc agaatcgaat ctggcatccg gacggggatc tgaatcttga
     3661 gggtatgaaa tacaatctgc ggatttatga ggagcagacc ggagcaaaag gaccatctga
     3721 ttacgccaag tacattgacc aaagctacct ctacgatgca ttgaaagagt tgaagaagaa
     3781 ataaacctgg agagtattga tgtggatgga aagcctgacc gtctgcgccg tgcagacggt
     3841 caggccgtga gttgggttat cgtttgtgca gagcgtcctt tagctcgccg cgagcgtctc
     3901 gcagctcagc ttgatcctgt cgaagatctc ttctgtccgt ggcgatttct ttgtaatcat
     3961 cgcggatctc ttgccgatct cgaaagattt cctctttgct ggcgccgttg cggatatctc
     4021 ggcgaagctc ggctctatcc tttctcagct ctttgaagtc accccgtaat tcgcggcggc
     4081 cttcctgcac gtcttttcgg gcgtcgcgta cgttgctgaa tttctctcga accactggtt
     4141 tattgtgatg acctcgattt ccctgatatt ttccagggtg cctatcacgc cggaagtgtg
     4201 aatcatcaaa gcgggcatgt cgcgtttctc cgcgacggta attaccgcgg cgattgaagc
     4261 gatgagcgtc acttcttcca tgcggtccat cgaatctccg ataatcgccg tcgcgcctgc
     4321 tatgcttgta tccgtcatcg tccttggcgt cggcaggtgc cggtagcgcc ggaagcatca
     4381 gcgctacccc cgtaagtccc gctagaatca ttttacttaa acgtttcatc aaatcctcct
     4441 tttgttcaaa tcttgctgtt tagttcgcaa agaaaattta tgcgttaacc ttaactgcgc
     4501 ggtgcgatgc tcgattagta ttcctgtcgc tggcgctgcg tctggacctc attacgattg
     4561 cgttcgatga cccgctggtt ctgctggatc tgctgctggt tgtattgata ctgagcttct
     4621 tcctgtcctt gcatgtaatc tccgacgagc gcgcccgctc cgaggccaac tccgccacca
     4681 atggccgcgc ctaatcccgg acggcccgcg gcggcaccga tcagcccgcc cgcggcggcg
     4741 cctccaactg cgccgatgcc ggcgcctttt tcccgcgtgg tcagggggcc gccggcgcag
     4801 cccacggcag tgagcgcagc aaagctcacc gcaatcaccc atcgaaacga cctaagctga
     4861 tttagattct gtttcactct gcctccaatc ttttcttgct gactgcattt agatgtaggc
     4921 agctagagca acgcacatgc cactagagca ccagttgtaa gtagcggata ttgccacgct
     4981 ggctcggcag tggaacgcac ccgttcgtgt aataattacc gagcgggacc gaaattgttg
     5041 cgccgactca cggccacgga cgaacggctc agccggtgtg ctcagcgaca ggcagtgagg
     5101 taattttgat ggtcaatcga ggacaagatt gcatgaaaag tcctcgcaaa ctggacgcag
     5161 tcaatccgca cgaaaactaa ggcgtacaat tgcacggtgg ttcctctgcc cgctggcagg
     5221 gaccatttta gataaagctc cggtggtaag cttaaggggc tagccagctg tcgatgtcgc
     5281 gaccaagccg tcgaaatggc gcgctaggat ctctgggctg ggacgttctc agctgatgag
     5341 gcttaaggat tgcgccaggg caacaatcat cggcaagaag tcttgaggcg aaatcaatcg
     5401 cgcgctgatc gaatcccaag acgcgcaagc tcgctcaagc ctaacgacgg gatcagcgca
     5461 ccttctttcg ttgttcggcg agaaatccgc tgctctcgag actgcgcgcg aagctgctat
     5521 ccataatctg ctcgatgtcg acctgcgcga tcttgggatt tacagttgcg acaatcctct
     5581 gaaccgattt gtaggccgct ggattgggcg caatgtcgag ggtcgccatc aatcgtaaca
     5641 ctttgtaaga ggcttcggct tcgtccgtct tctgcaggcg aagatgcttt tgcaaggttt
     5701 tcataacctc gggcttgttg ctggcgtcga gaatgtaggc ggtcgcctcg actagcccct
     5761 tggtcacgcg caggaaaaag tcctgtcggt tggcaatgag gtctttgagc gcgcaaatgc
     5821 tcggtccttg atagggcgct ttcagttccg cggcatcggc cagcgatcta aaccccgctt
     5881 tgagcggcgc ttcacttaag ctgtaggtca gcgccgctgc atcgatgttt ccgttcaaca
     5941 atgcttgggc gatcaccggt ccatcaccga tgacgcgcat cttcaatccg tatttgtccg
     6001 gctcgacacc aagatgatcc aggatgatca tggtctgcag ccagaaccca ccgccgatgc
     6061 tctgtacccc aatgatgcgg ccacgtaatt cctcgatggt tttgatttct ttacgggcga
     6121 ctagctggcg cggaatttta ttggtggcgc tggcaatgca gcctagcttg accccgccgg
     6181 ccaccgtgct cagaattgtc gattgggcag gccatacggc ttcgacctcc ctcgatacaa
     6241 gcgcagcgat gctgagcgag ccgctgcgta cttgaatcat cgagccatcg atgccgtatt
     6301 tcttgaaaaa acccctatcg atgccgaccc acatcggcgc cgctgtttcg ttatagccac
     6361 cgtaggagac gcgaaaattt tcttgagcat aggcagccat agccaggtac atcgtagaga
     6421 tgcatagaaa cgcgccaagc agactagcaa ctcttccggt aacactcatc gagtactcct
     6481 gttattcaaa agcgcgatgc gcaagttgtt tcgacagcct caaacctcaa gcgttcgaat
     6541 agttggaaaa tggccgcgtc gttgtcgcat tatgctcggg tcccatgatc atgaatagct
     6601 ttcctcttat acgacttttc tttacaagaa aacgctcgct ccgagtttaa atactttagc
     6661 gtgtgtatgc gggcggagtt ttcgtgcgcg agcttcgggc gactacagcg atgatccgtt
     6721 atcgcagttt cgcttatcgc ttaagccggc gcgattagcc tgattgttat ttacgataat
     6781 ccggcatgac aatcggggga cccagtgatt gttacagcgg atgcaccgtc ccaaagcctt
     6841 cgaaaacacc ggttgagatg ctgcccgagg tcagttgtcg gatccgtggc agcttaatga
     6901 ctccaaggta cttgcttggc ggagactgct ccggccagtg atctggcgag agatggccgg
     6961 cgtttagaaa aatgcctgca ggtttaacac aagctcctgg gtacgggctc gctgattgaa
     7021 atcaaatcgt aaagtgaaat ttctagtcaa ggtatagcgc tgctgcgccg atagccgcca
     7081 ctcgtcagat ttctctccaa gcggaaaatt caggtagcca ccggaaagtg cgaatttcca
     7141 tcgatcggta atatcggata gtgcgccaac cgtaaggccg ccgcccaccc ggtgattgcc
     7201 gtggaagacc ggccccactt cggtagcaaa ttccgccagg ccgaaataga cctcgcggtt
     7261 taaccaggcg ctttccctcg ctacaccgat tccagcattt aggttgccgg tgcggcaaaa
     7321 gcgacagttg tgatgttgga tcgtgttgag cgccgcgttg atcttccatg acggactcgc
     7381 aaacagagca tccatgggcg acagagacaa aatgtcgagc agggtgaatt tttcgagcct
     7441 ggtatgatcg ctacgagcgt agtgccgcaa ggtcagcccc agcgcctgga tttgcgcacc
     7501 gggcgtatag ccgtattccg gatcaagcag gtcgtgaaaa gccaggcgaa aatttgcctc
     7561 gttgaagaac tcgccttcac gccagccgaa gcccagcccc gctctcatgg tcttatgccc
     7621 ttgatcaggc gcaccgctag cgggcgcaat gtctaaggga acgggtcttg cctttagctt
     7681 gctgcgagca agcaaaatgg tcctattacg cgattcgaag gccccgccgt tctccgccgt
     7741 agcgcttttg tacaaaagat aatccgatac aacatccaag actgccgctt gacgctccgg
     7801 tggcagatgg gtgaaggaat cagattgaga aagcttcgga tcggtgacca tcttcgacag
     7861 ccagttcctt tcttcggctg aaagagcctc ccgaccgcgg cgaatcctgg tgctccggga
     7921 ggcgcgaaag gtcacatcat taaccagacc gggcttgtcg gcgatcatgc ggatcgcatc
     7981 ggaagggaag gtatagaacc agaaccgttc tgctagttgc agctccggat cagcgacatc
     8041 cagcagagcc aatatctgat aggcgcaatt ttctttgaaa taaaagtaat cgaaagtggc
     8101 gctgcccatc tcccaggcgt gcatcagcag tcgttgcatc tgatcttccg tgagatcgag
     8161 ccggtactcc cagatgtccc ggttttcaaa atcgccgtat tccttcgctt tgatgtagta
     8221 gggatgtgtc gaaaagtatc ctttgaatcc tccagtcagt cccaacacgg cgaagaggat
     8281 gccgttgtcg gtggtgacat cggccgcata gttgatcgta tacgccagaa tgctggttcg
     8341 ctccgtttgg cccttttggt caacccgcaa gagcaggtgg ccgaacatcg aagacggatt
     8401 gttcataaac gccgagggaa agatcacggt gatcgaggcg ggattgagct cggtaatcca
     8461 tgattgaaat tgctcgcacg gttgctgcgg catcctggtc tcatcgatag ccagggtgct
     8521 cttcagccag tgataacgcg cgataaaagc gcaatgcgcc ggttgcggcg agcgcccgac
     8581 cagatcggtc gaaaaaaatc ttgtgagcgt agcctgcagt tcggcttccg gatcggtttt
     8641 tccgtccggc gctaaaaaga atcccggttc gtcggcttcg ctctcaaccc ccccgccaag
     8701 gctcgagcgg tagtgcagaa ggagatgcca gtagcgttgc tcgtgcagcg ccattgcttt
     8761 agcgcgctgg agcagttgtt cgaggtaagt gttaccgctg tcctgggcgc cggccgtgtt
     8821 cgggagaaaa cacaggaaga agaatgcaat gcttaggatc gaggacaaga gaaaaagaaa
     8881 gccccggcga ttggccgggg ctcgtatatg atttacttca gactgctggc gagaagcggg
     8941 tgcgatgaca tggcgctggt taaagccttg agcaacgccg ccggagtaga ttcgccggac
     9001 tgaatcatgc tctgatactg cagttgagca agcgcataaa actcggcgcg gttttcctgc
     9061 gggatgttca tcagttcagc cagtgagctc aggtgttcgc cgtcgccctt ggccatatcc
     9121 tgagaaaggt tctcaaaatt gagcgccgca aaaacgctag ttttgtgttc ggacatgatc
     9181 acgccgttat tggtgcaacc ggaagtgccg aagctgatgc cgaacgtctg gctgccgaac
     9241 gtcccattag tggtcgactg catcacctgc ggcgcgatgt ccttctgatt tccatattcc
     9301 ccccaagcga gtttaccgag accgcaaccc ggtccggtat ccgggtttgg tgctccccaa
     9361 acctgcgccg cgtacaaagg tagcagagcg acgcccaacc tgagccattg ttttttgatc
     9421 atagtttcct cctccttctg atgtgttcta taactttgaa tgtagcttga acaacatatt
     9481 cgagcaagga catttttcaa gtaatctgaa aaaactcccc cggtgtctgg acgatcgttg
     9541 acacgcatcg gtggcatcgg tatcgctgtc caagtaaacg ctagcgcggg ggtagaggac
     9601 ccggcccggc aaattttttg agcatgttta tggtctcggc gtcccggggc atctctgcaa
     9661 cgacctttct cagttccgcc gctcccattg gcgatgcatc atcggtgacc aattttttaa
     9721 agtagggagg aaactcaggg tctttgaaga tcttgtgcat tgcttcctcg agaatcgcaa
     9781 ctctctcttt gggggttccc ggaggaagta tgtaaggtga tcccaccgat cgaaaagcgc
     9841 gccagagtgt gagaagtttg ttttcctgat cgtttttggc gaagctaccg atctccggaa
     9901 ggtgacctaa ccgtgggtgc ttgtcacctt tcggaacctc cataatcgca tggaaattca
     9961 tcagcccttt ctccacccaa tcggagtttc gccggatcac cgagcttgcg ttattggccc
    10021 tactgtcgag ttcgccttgg gtcaaggcga cgtccaattc atgggcagtg taaccggcga
    10081 taaatctggg atcttttaag ttcagaaagt acgcgaagag tcttcctgct acatacgaca
    10141 catggccgac ggtttgcgct ccgattctca ggccaggctt ggatgcgagt ttctccagat
    10201 tgtcgagacc gagttctttg cgggtgtaaa tgatgtagtg gttttcgctt tcgggtgagc
    10261 caagataaat aaacttgtcg atgtcataag tgatgccggc ttcgcccatg acggccaggc
    10321 cgataatcgc tccgctcgcc gcgcctatgg tcaggccgtc gggacgaacg ttgctgtaca
    10381 gagaattcgt tcccttgcgg ccgccgcccc cgtcaatgta ctggatgacc acggtcgggt
    10441 ttcccggaat atgtttcttc aggaaaggcg ccatggcttt gacgcggaga tcgccggtgc
    10501 cgcctgccga ggtggtggcg atcaaggtga cggtcttacc ctcgtaaaag ttactttgcg
    10561 acagcgcatc attcgaacag atgcaaaaaa ctagtccata gaaggaagcg atccggatga
    10621 acttgttccg ggtaatcata gaatctccgt cttgctggta tgtctgataa ctgtcattcg
    10681 atagcacctc ttatctaaaa agtcgcgacc ttttacaagc ggattttaaa ttatcgccat
    10741 gcctgcagat gagggaccct gggggccgcc gctgtgcatt gaatttgacc gctagaacga
    10801 ggccgtgtta gaagccgagt tatgcaacgc agtagcctcc gcgacgagca cgaatgaagg
    10861 caatgacgat ggtggtttcg gccgatggtg ggcgagcgct acggggagac atcatgcaga
    10921 tcgagttgaa ctatcccgat ccggttccaa ttgaactaga cccgagtaaa acgttgctcc
    10981 tcgtcgtaga tatggagaac gaagacgctc atccttcggg caagcgcttc gcaggccaag
    11041 cggtgactcg catcatcccc aatatcgcaa gattgcggcg caaggtgcga caatcaggcg
    11101 gatccgtagt tcacactcaa tcggtgcgca aaccggatgc gctggaattt accgtgttca
    11161 ataacgcgat tcgaaaaatc gaaggtacct ggggtgcgga gttcgtggac gagttaaggc
    11221 ccgaagccga tgagccgatc gtggttaagt acacgcacga ttgtttctat cgcaccgaga
    11281 tggaatctgt cctggaggga ttcaatctgc ggccgggaga tgcgcgaatc attgtgaccg
    11341 gtatctcctc gagaagttgc gtgcagtgcg cggtgaccgg ttttagcatt cgtagctatt
    11401 atgtttatgt tccaatcgat tgtaccgggc agcgcgaaga ggaggagaag ctgcaagcat
    11461 tttctctttt tgccagcttt gcctatcgtt ataacgtgac cgtcacacga tccgacttga
    11521 ttcagtttag ctaacggcgc caaaaatcgt atccgatcct gtgtccacga ggcgaactat
    11581 gggaatgacc atcactgaaa aaatctgcgc gcgagccgcg gcaaaggatc gggtcggcgc
    11641 gggagacatg atcgatgcgg caatagacaa actctacatc aaggatttgc gattttcgaa
    11701 agcggaagac cctaaaggtc tgtacgggat tttcaaagag atccttaagg acatgggcat
    11761 cactaaagcg tgggatcctg acaagatcat tatcaatttc gacgagcaac cggcgcgcag
    11821 cacgagacgc gcggaaggtc aggagcgggc gaaacaattc gcgggagagc atggggcgac
    11881 ggtctatgaa ggttaccagg ggggcatcgg tcataatgtc atggtcgaaa aaggtcatgt
    11941 tgtgccgggc gagtttatcg tcggcgccga ttcgcattcg tgcacctatg gcgcactagc
    12001 ttgcttcgcc accggcatcg gttttactga aaccgtcgga gtgcttgcaa ccggaaggat
    12061 ctggcttaaa gtgccaccgg ccattttcat cgagcttacg ggcaagcgcc cgccctggat
    12121 ttggggaaag gatatcgcgt tgcgcattat gggcgcgatg ggagctaacg gcgcggttaa
    12181 caaaaccatc gaatatggcg gcagggcggt tgcccagatc agcatcgaaa gccgtctctc
    12241 gatgtgcaat atggcggttg aaagcctcgc cttgaacgcc gtttttccgc cggatgaaag
    12301 cgcgatcgat tacgtgaagc aagtcagcga tgcaagcttt gctgcggtcg aaagtgatgc
    12361 cgatgccgaa tatgaatcgc ggcttcgctt cgatttgacc gatatggagc cttttgttgc
    12421 cgcgccgggc gtgccctcca acggccagcc ggttcacgaa tttgtcggca ccaagatcga
    12481 tcaggccttc atcggaactt gctccaactc acgtatcgaa gacctacgcg tcgcagccaa
    12541 aattctcaaa ggcaggacgg tgcatcccgg agtgcgcatg atcgtcacgc cagcttccta
    12601 tgccgcctat ttccaggcac gcgaagaggg acttctgcaa attttcgaaa aatcaaaagt
    12661 tttgctcacc gcgtcggaat gcggcatctg cacgcgtccc tcgctggcca gcggtgaagt
    12721 ctgcatctcc tccggcaacc gaaactttcc cggccgcatg ggtcaccgcg atgcccagat
    12781 attcttgggg agcccggcga cggtggccgc cagcgctatt gcaggcgaga tcgtcgatcc
    12841 cagggagtta ctgccatcat gaaccgaatc ttttccggta aagtttggaa attcggcgac
    12901 tgtatcgact ccagcaacat ccgtgtgcat ctcgtccggc ggccgaaact ttcttggacg
    12961 catgggtcag gcgatgcgca aatatttcta gctagcccgc catgatggct gtcagcgctg
    13021 tagcaggtga aatcttcgat cccaaggagt tactgtcagc atgaagcgaa tttttctcgg
    13081 taaagtttgg aaattcggcg actgcatcga ctccggcaac atcaaaggtg tgatggccgg
    13141 catcgatccc cagtttcact taaaagtgaa gcccggagat ttcattatcg ccggaatcaa
    13201 tttcggcatg ggttcgagcg cggaagatgc gccccgatcg ctgcgcgagg ccggcatcgc
    13261 cgctgtgcta gcggaatcca tatcgaacat ttatctgcgt accttgatca acctcgggct
    13321 gccggcaatc gagtgccctg gtatatcgac gcttgtcgca cagaacgatg agatcgaagt
    13381 cgacttgaga tccggcacag tttgtaattt aacaaatggg gcttcactcg cgtttccgcc
    13441 tttttcagat catgcgattg ctattctgga gagaggcggt ttgattccgt acttgaaatc
    13501 ctcattcgcc aagcaagaca aaagcgcgcc gctgaattcg taggaggagc aatgaaagca
    13561 acgactaagc ttagagaact gctgaagggc gatgacatga tcatcactcc cggggtttat
    13621 gattgcctga cggcgcggct cgccgaaacg accgggttta ccgcagtcaa actgttaggc
    13681 aatgtcacct gcgccagctt gcttgggctt cccgatctgg gtcttatcgg acttaatgag
    13741 atggccaatc atgccaagaa tgtggccgct gccgtcgaca tcccagtcat ggtcgacgcc
    13801 gatacgggat attcgggcgg accgcttgga atcgcccgga cgatcaagga attcgagcgc
    13861 gccggtatcg ccggcatcgg catagaagat caggtgacgc ctaagaagtg cgctctcatt
    13921 ccgggaggaa ctccggttgt gtcgatcaaa gagcaagtga gaaagctcca ggccgcggtt
    13981 gaagccaagg aggattcgga ttttgtcatc agcgcccgga ccgatgcaga gtcggtgaac
    14041 gggttggatg atgcgctcga tcggatcaag gcctacgagg aggtcggtat cgatatggtc
    14101 ggtgttgcca tgtcttgggt gcgaaaggaa ggcatgaggc aacgaaccct ggacgcgttg
    14161 aagcgtatcc gcgattcggt gaaagtgccg gttaattgca tgttcatgga cgaggcgatt
    14221 caagtcggca acgttacgtt ggccgagctt aaacagatcg gtttcaacat gggcgggagt
    14281 aacgcggtgc gctacacggt gatcaaagcg gtcacggaga tgctgacgat tttaaaaaac
    14341 gagggttcga ctaagaacta tgcgcacagg ctggcttctc tcaaggagta tgaggccttg
    14401 gtacgcttgc ccgagttctt ggagatggaa ctgcgttact ccatctaacg gaccgccggc
    14461 tagaatccat ggtgctcatt cgatcatcgc agctgtttgc gatttcgcgc atatcgacta
    14521 tggattagtc gatgatgcgc ttgcggcctc tttatcgtgg cccgatacga aggctgatgg
    14581 tgcctacata gatctcgcgt aaaaaccaca gtagcccgag aacgaatgtc gacatggcgg
    14641 caatgaaaag cagggcgacg accacagagg cgtcgaaccg caaaaatgcg cttacgaaca
    14701 ggaatgcgat taccgcgcaa acgagcaacg ccgtcgtcgt gcaaagagcg atcgccgaat
    14761 agatgagctt ggcgcgctgc gaaagcagtc tgagatcggt ttgcaacatt gaaaagaatt
    14821 ccggagacac atcgactaac tgctcttcga gatatcgggc gcgatcaatg atgcggccta
    14881 atcggttcgt cataaccgct agcatcgcgc cgattcctga aagaagaaat accggcgcca
    14941 cggctagctg aatcgcgtgc gccacggtaa catcgctgaa ctcaagcacg atcacgttcc
    15001 ccagccagca tcacgctagc accgctccac aacgacctaa atttttactt tagtgaaaac
    15061 tttcaaagga tttttttgaa atattttcag cttgtcttca gcgctcaaga atccgaggcc
    15121 atctatcact ggaattagat cgtcggatgg catgccggtt tcgggacgaa tagcgccgcc
    15181 ggagcccgga gattcggtgc caaaaagaat ctgatcgatg ccgcgctgct tgatcgcggc
    15241 ttcgaggtag tgaacatcgt aaacgcagga gtcgtagaag agattgttgc tgaaatctcc
    15301 ctgtccgacg tgcttgtcgg tgggaataaa ccggttcagg ccgccgccgc agtggcagat
    15361 ggtaattttt aattttggaa agatctcaaa gactttactg tgggttagta gctgcatggc
    15421 gacaaactct tctatatagt tgttcatctg atagtttgcc ggaatgattt ccagtctccg
    15481 gtcgtaggtt actgacggat ggatcatcag aggcacatcg agctccacgg ctttttcgta
    15541 taacggaaac caatactcct cgtgcacgcc tggagtttgt ttgtcgcctc ccggatcggg
    15601 gttcagatat gccccgacga agcctaattc tttcacgcag cgctcgaatt ccggcagaca
    15661 gttgcgcgtg tccttgcttg tcgaacactg tggcaactgg gccatgccgc gaaaccggtc
    15721 ggggtggagc ttgacaatac gcgcgatcac attgttggtg ttttcgctcc agaattcctg
    15781 taggaatggc cgcatccagt gccacatggc gattggcctc ggtccgatga gctgaacgtc
    15841 gacattgcga tcgtccataa atttgagatg acgctgctgc gcgttctcca actgctcgtc
    15901 ggtgacctcc agtcttcgcc cgggcgtatt ttgcgaaaca atttccgcca aaaattgccg
    15961 aaaccctgcc ggggtggtca tgtggccgtg aacatcgacg acggtttgtc cgttgtacat
    16021 tagaactcct cagaagtagt cagtgttccc ttacataaca gagctgaacc tggctctgtc
    16081 aagcggttag aattcgggca agaggctgct ctccctggtg agccggcgat gaattaaggt
    16141 ggcggctgga acatcaagat aggttgctac atagcaatca gaacggcgat gactgctagg
    16201 aaagcggcgg gagagcgcct ctcactcgat tcggcgcctt ctccgcaggc ttctattggc
    16261 gcactgtgag caccgggcag gtcgccgtcg atacgactcg gccggcaacg ctgcccataa
    16321 acaatttgga tagcccggtg cggccatgcg tgccgatcac aatcaggtcg gcttttttgt
    16381 ttttggcggc tttgatgatc tgctcatgcg gcgatcctct cagcagcagg ctcttggctt
    16441 tgattttagc cttctccagt gtcgccatga gccgcttcat cgaactctct gcctgctgtt
    16501 tggcgctttc ttccagcctc atatagagct cggcgccgcc aaattcttgc tcggaaaggt
    16561 aagccgttgt cggcgcgatc acgtgcacca cccagagctc cgcgtcgtaa tctttggcaa
    16621 gctcgaccgc cttttctctc gcgcggcctg atgctttgga ataatccgtg gcgtataaaa
    16681 tcctgcgaat gccgctcacg atgtgtgctc ccttagttgc ttctggtttc aagccgaagc
    16741 ttgaaccgcc tgcgctgcga gggccgtctt ggcaatgccg gccggcacct tcgacaggtc
    16801 gaggttgtat aacttacaga cgttggtcgc cagcactttt gtttgaactt ccacaggcag
    16861 gtgcccaaga tcacgttgga taattttcaa cgaattaggc caagtcgaat taggatgagg
    16921 gaagtcgttg gaccacataa tattatcttc gccccaccat tcgagattgt ggccgcacac
    16981 cgagtcgttg aagaagcacg aatagatttg attgcgaaag tactcgctgg ggtccttgct
    17041 gaaaggcggg ggattggcct tgcgaaagcg ccggtaatag taatcccact gctgcgtgat
    17101 ccagggtaac caacccgctt cgttctccac cgaaaccatc ttgagccgcg gatagcgctc
    17161 cagcacgccg tagaaaatga gctcgaaaag cgcattggcg atgtccatca atttcaaatt
    17221 aacgctgccg cggtaccgct cgacgccgtt gcggttcttg tctttgttgt agctgtgccc
    17281 agttagaatg tgcaaactga ccggcgtttc gaggtcctgg gcggcggccc aaaatctctc
    17341 ataatggttg gaatgaaacg gcagatccgg atgcggtgct tgccaaatga tcgccccctt
    17401 gagtccggcg ttacggcaac gctcaagctc tttgataccg ttttcgatgt tgtagacgga
    17461 aatggcaggg atgccgatca gccggttagt atcaacgctg caataatcga ttaaccaatc
    17521 gttgtacacg cgaaaacagg cttcctgcat gcccggatcg tcaagcgcga acagatccaa
    17581 catcagtgtc ggatacagaa cctcgccgct gaggccgtcc ttttccatct cctgaattcg
    17641 tgcccggggg tcccagccgc cggcgtgttg ttgaaagcct tcgccaactt tgtgcggcgg
    17701 aaagagcgga gcgtcttcgc ggtatttttc cggaactccc tttttccaaa ggtccaccgg
    17761 ttccatgaca tgggaatccg atgagatgat gacgccattg ataccctgac cgtgaccgtt
    17821 tccgatagcc atctgtgagt cctccttaag gaatcattgc tcactgtact ttgcagtcga
    17881 ccgaacttat gcgataaccc gacgacatgt caattcgaat cgatgtcaac aatcgccgag
    17941 ttggtttccg gcttgcgcca gagcgaagcc agccacggct gctcggctcg cggcgctccg
    18001 ggcggccgat agtaatgagt gatctcagta aagccggctg cagtcatgaa tttacgccat
    18061 tgctcaagat tgtaataggc gccgtagcgt ttgccgttcc atccttcttg attatcaccc
    18121 cgtggattgg agctgaagag cacgccctgc gctttcaacg tctgccatag ctcctttaga
    18181 acgcgcggca gttcctgact ggggatatga aagagcgaag cattggcaaa aataccgtca
    18241 aaaaaatttt ccggcagttt cagtcttaga aaatcctggc gccagacttc gcacccgctg
    18301 aagtcgcgcg ctatttcgac aaaacgttgg gcgccatcga gcccgatcac ttcatgtcct
    18361 aggtctttga acgccttcaa gtcgcggcct ggaccgcaac cgaaatcgag aatgcgaaag
    18421 gaaccttcgc cccgcatatg ctcgaggagc gcggcgcggt tttgtgcgac gtcatgatcg
    18481 cgcgtgcctt cccagaaact atccgcgtgc tgattgtagt gatcgagggt gatacttgat
    18541 atcttggtaa gttcgttctt ggttcccgca cgcttaaggg ccatgttcgc tccgcatggc
    18601 atcatggcaa caggtcacgg aacattcaag catggggcat ctggagctct gaggtggttt
    18661 gagcagcgtg cacatcgaag taggaaggta ttttgataat ccagcaattc tccgcgaaac
    18721 gtttctgacg gtttgaaccg ctcgaacgtc tgaaccttta tttcggaagg acttccggat
    18781 tcaacagctt tagcggacgg ccgtcgagat agtcgagtat atttgccatt gcttgttcgt
    18841 agcgctcgcc gagaacctcg acagtggcgt agccgcgatg cgacaacaag acagcattct
    18901 cgaatttccg taagggatga tccatcggca ggggttcgag gtcaaaaacg tcaagcccaa
    18961 cgccgcccaa ttgaccgctt tcgagcgcgc tgatcattgc cggctctgag acgatcggtc
    19021 cccgcgccgt gttcactagg aaagcgccgc gcttcatcat ggcaaattct ttttccccga
    19081 taatccctcg agtttgggca ttagaattga tgtggatcgt aatgatgtca gactcacgaa
    19141 tcagcctttc aaagctgacg cactcggcgc cgacttccgc cgctttttcc ggcgttagag
    19201 ttcgagagta gcctaaaact cgagtattaa gagttctcat aacccgggct acttctttgc
    19261 cgatcctgcc aaagccaaat accccgaccg tttttccctc cagagtccag cccggaatcg
    19321 cgggccaatc ctcggtgcgc atgcgccggt cgacctgcgg gatcttgcgc atcaacgaga
    19381 gtatcagtcc cagggtcaac tcttttgtgg tggcgccggt gtcggctgcc gtgcccgata
    19441 cggcgatgcc atggcgggtg gccgagggca aatcgagatg cacgctggtg cgtccggttt
    19501 gtgagatgaa tttcaagttg ggcaactggc ggtattcatt gccggtaaat gaggtccgct
    19561 cgcgcatcag caggattatg tcgaaaggtc gcaacgtttg gacaattttt ttggaactgt
    19621 ccaatctttc tcgcaagagg gtgatgtcgg cacgggcctt aagtttgtca aaggcgggaa
    19681 cggtgtcaga gattttttcg aaatcatcga ggatagcgat acgggcgcgc ccagtctcag
    19741 gtttactcat ggtaactccc agcacaccgc gatactccac aaacgggatc tcggaagcgc
    19801 ttgtataaag gagcccgcgg tttcattctg tcgtcgccgt gacttcgacc tcgcatagtt
    19861 ttccgaccga cgagtaaccg tcgacaaagc agtattcccg ttgagctgtt gatgctttga
    19921 tgcgttcatt gaatagtcgg ttcaagttat cgatggtttg gcttcggtgc agatagaagg
    19981 aaatccgcgc aactctctcc cacgaactgc ggctgtcttt caaagaaccg gtgatccgcg
    20041 gcaggatatt gtccaattgt tcttcgagtg tcggcaggac agtcgtcact ccggagagca
    20101 tcaccacaga atcgtagacc aggaaccgaa tcggcacgat gggcgggtcg tactcgacga
    20161 tgcgcttttg catgttttgc ctggaaggtt tcatcgccag caggtccacg gcgatctttg
    20221 catcagaatc aaaatgatcg ggagcgatgt aactcgagct tgccgatcgc gcttggccgg
    20281 aaagcacttt aacccgctcg tcgctcccta ggtttcggct ttcacggtcg cgaccccaca
    20341 gccgagtacg tacgacgttg tctagtgaca acttatgcgt tttgagctcc tgatcgaaac
    20401 gacggaagag ttccgaggct tcgttgctcg cgcttgcccc aggtttggct tcacccgaga
    20461 gctcgataaa atctttgcca agccacgtaa gaaatttctt gcgcatgact gatctccttt
    20521 attctcacgg cgcgctgacc ccatgctata tcagccgatg cacagagttg tgaagaggcg
    20581 cgtgtgcgaa attgtgaagt tgcgatgaat atacgagaac ggcaggcaag ataattgtca
    20641 atcgagagct ctggagattt tcgcaacggg aagacactta gattgggaat ccgcgatcaa
    20701 tttggctaag cgatagtgcg agcgggacgt gatctcggct agggaatgct gaaattccgc
    20761 ggttaccccg gactacaaca aaacatttta gctgattttg gggcctgcgc ctccggagct
    20821 tcccagatag tgcgaagatc ggcagagtac ggatagcgga taccattctc ttcgaggatc
    20881 ttgccggtgg cttcgataag ctcggatcga ttaaacacca ttgtttcatg ggtcagcact
    20941 ccggcgctgg cgaacttgaa actgatttgt tggtcgcgcg aatcgcgcag tatctccact
    21001 aggtgtcgca gatttctgac gcgaatattg ttgacttcgc taagtacgcc ccgattggga
    21061 tcgtcatagc cttgagtaat tcgatgtgga aacaggggag aggcaaccat gacgagctct
    21121 tcgtcatcga atgctggacg atcatagcgc cgggtgacta gcgggctggc gccccgtcca
    21181 aaactgccgg ccgaggaggg acgttggtta ccaagccgtt caagatagtc ttgcgtagtc
    21241 tgggagaata caaaagggcc aaagatgaaa tagcggggac tcgaatttaa aaggtaagga
    21301 atgaccagct cacgctgtgt tttaacaggc aaatcgattt tagccagctt gccgtctcga
    21361 taaatcgtta gtggcaacac accgtttttg gcgaacttct gtgttagata agaggctgac
    21421 aaccggagat catagcgcac tgccaccttc ccatcgctgt cgatcggagt gccgccgatg
    21481 tgcgtgatga cgtcccactc cttcagaggg taagcatcgt catcccgata aggttgtgcc
    21541 accatgactc cgcttagctc cttaggcaag ccgagccttt gccggagcgc tgcattctct
    21601 actgtttgta tcagatcgtg cgtttgcggt ttgccgtcgt atacgccgtc cttcacatcg
    21661 tcgagaaata gttgaatctc ctccacaggg atcagatagc cgatgttctg cgcattttga
    21721 atcaaactaa acaccaaacc gacaagcttt ccgtccgaga cagcgggtcc accgctgttg
    21781 ccgaaattta gcgccgcatc gacctgaatt cgaagacctg ctgcctgata gtaataatcg
    21841 gtgtattcga tgcgggaaac aattccttgg gttacggaaa gctctgtgcc gccggtcgga
    21901 tagccgtata cattgatgtt gtccttgact cgaggcagct ctttggcaaa agcaagtgac
    21961 ccgcgcttct caaaaaaagt ttcatcgtcc aatttcaaaa tcgccaggtc catagatggg
    22021 gtaatcgaat caacccttgc cggtaaatgc tgcgacgact gattgggctg cacgtagatt
    22081 tggctggcgt agcgaaccac gtgcgcgttg gtgagaatgc ggttgccgtc tatgaccaca
    22141 cccgaaccct tgatctgttg cggcgtattt cggctccatg ggcgcaacac atcgggagtg
    22201 tgatgaaccg cgtggatctt gacgaccaaa tcacggattt ggtcctgcgc actcgagtct
    22261 gacgccttca ggttaacgat aagcagcatc accaaaacaa tgccgagtcg gcccataaac
    22321 tctcctgata atttaaccca aattcgccgt agtatacgcc aaagatcggc tgagttaaat
    22381 gatcgtttca accttgacct ttgaatctat agcctggtct agtacaccca aacagatcga
    22441 tttgtatgcc tgataagctc attgcattct gaccgctgtg tcacgccctt tcacggttag
    22501 caagtagttg ttgaccatct tgacatggtc aagtgcgacg acgtatccat cagttgggtg
    22561 cgaaacgtct ggagccgcaa ctgaataagg agaacgccat gtggtattta gtgatgcgtc
    22621 gcccggtaaa gccgcgggag cagtggacag tcaacctaga ccaacacctg gtctggatga
    22681 agcaacagca cgacagcggc cgtattcttt tttccgggcc tacgccggat agaaagttcg
    22741 gcatttatgt aattaaggca gcatccagag ccgaggccga ccggattgcc ggcagcgacc
    22801 cctatacggc cgcgggtttc tgcgagtacg atctctatga gtgggatgtt caccagatca
    22861 tgggcgcggg gccattcagc gcggcggaac tgcgtaccca ccgctaatta gcgatcaaga
    22921 gcgactttgt agaggcataa tccggaagct tcactcgatt caccggtttg ggacttccaa
    22981 acccttgatc gcaagcactt cgatgccgcc gtcctggtat acttttacag tctggttgct
    23041 agtgtccttg taagccgtcg caataattgt gtatcggacc tgtaaatatt ttatcgccct
    23101 ggaccgtcat gctcatcgcg ctgctcaccg gcaaaacttg acttgataat tagcgacgct
    23161 tcttgttcaa cagcgtagcc tctttgcgtt cctaatctga tgcccccgat cgaaatggca
    23221 tcgggttttt cggcagcaaa gatccccaaa ccagatccag ggttgcgatg aggcagtctc
    23281 ctggcagcaa gctttacatg aattataata agcgatggtg gagattagat aacgcctaaa
    23341 tactcgatga tcgtgtttat cgccgtttag tggatgtgat caggggctga tgtcaaaccc
    23401 ctgctgtcga tatataataa agaccagccg gctcatcgtc cgaaccaggc cgatcttgct
    23461 gccggtatcc ggtattatga accgagtcga ttagggagct gatgtactag cttaagtgga
    23521 agttctagat ggatgatctc gtcgagcgaa tcttaaatgg acagaatcag gacgggggct
    23581 gggggttttt gccgggcaag agtagtaata cagaatgtac ctctctctgc gtgctggctt
    23641 tggacagtct tggaacaaag cagccggtgg caagcacaaa acgaggtttg gactggctgc
    23701 ttcgacatca aagggccgag ggcagttggc cgctgaatga ttcatccgtt gatgcttcgt
    23761 ggacaaccgc tctggcgatg atagcgttga accgattccc gggacagcag ctacaaatga
    23821 agaccgcctc tgactggttg ttgaaacagg agggtaggac tccgggcata ttggctcggc
    23881 tgatcggggc atttatgact cctagtaatg ccgttgcttt gaatcccgat ctgaaaggct
    23941 gggcttgggt tccacgaaca ttcagttggg ttgagccgac cagttatgct cttattgctt
    24001 tgaaacaaac ccgaaccgcc gttggcacca agattttagc ggagagaatt caacagggcg
    24061 agttgatgat ttatgatcgc atgtgcaatg gcggtggctg gaattacggg aattcgaagg
    24121 tttttggcga ggcgctttgg ccttatccgg acgtgaccgc agtggcattg attgcgctgc
    24181 aagaccatca ggctagccaa gccaatcaag aaagtttgac ggccctgcgc aggatgatgc
    24241 gagaaaccga ttcgggttta gcgctatgtt ggggtactat ctgcttatcg atctacggcg
    24301 gcgagacgtc ccaatggaag cgccaaatcg aaaaacgcta tcaagatacg ggttttctcg
    24361 gggagatcaa aacacttgca ttatcactta tcgcctcggt cgatgatgcc aaggcttttc
    24421 gagtttagct catgattagc cgtcggagtt tcctcaaata ttcaaccctc gcttcagtca
    24481 taccgttcgt agacggctgt agcgagagtc aaaagtggcg cgcagaagca tatcgcaaac
    24541 agaggtcatc gcaggtggcg atcgtgcaag ccttgcgcta cgacttatca ttgcgcgaga
    24601 tcataaccaa aggcgtacgg ctctgcggct tggaggtaaa aggaaaaacg atcgttctca
    24661 agccgaatct ggtggaattt gatccaggcg gtgttatcaa tacaaatcca ctggtgatcg
    24721 aagccgcgat agattgcttt cgcacactcg gcgcgcggca agtgttggtc gctgaaggtc
    24781 cgggacatcg tcgagataac gagtatctcc ttgctgcgtc tggcatttat gcggtaatca
    24841 aagagcatcg cgtcgcttac gtcgatttga acaccgacga tatacgcccg acccgccttc
    24901 ttagcagttt cacgaaactg gagcagctct acttgccgga aacgctctac aacgccgatc
    24961 tcattgtatc tatgccgaag ctaaaaacac accattgggc gggcgttaca ctttcgttga
    25021 aaaacatgtt cggtgtggtt ccaggctctg tttatggctg gcccaaaaat atccttcact
    25081 ggcaggggat tcacaatagt attctggata ttaatagctc cctacctgtt cggcagtttg
    25141 cgattgttga tggcatagtt gggatggaag ggaatgggcc tctacaaggc caggctaaag
    25201 agagtggtgt tttgattttt gggaaggacc ttgtagcagt ggacgcaacg gcagctaggt
    25261 tgatgaaaat tgagccgaaa aaaattaagt atctcgcgca agcgggcgag tttctgggaa
    25321 atgtgaacca cgagaaaatt gaacagatcg gtgagaacct ggaccggctg caaaaggatt
    25381 tccaagtgat cgaggaattc caggatctaa aaacgttgac ctaacgggtc caactctcct
    25441 acggcctcgc aaacagttag ggacccgaaa ctacaggtgc gaagttttgt cgttctttca
    25501 gatcaacttc atcaaaccta gagcccaaaa cagaggggcc gaacggtatc gatccgttcg
    25561 gccccttctg ttcgatggct cttctgctga ttagctcgct tgatcgtcgg cagggattat
    25621 cagcttcatt ccgacataga tgtagttagg atttttcagg ctttctttat ttgcttcgaa
    25681 aatcttctcc catttagatg tagaattata gaagcgctcg gccaatttgc ttagggtatc
    25741 gcctacttta accacatatt gctgatctgc tggcgtaccg gatttcaccg gcttcgcagt
    25801 agtagctgct ggagctggag ttttttccga tgccgttgtg gccggtttag cgatctcact
    25861 agctttactt tctgcggctg ccggttttat cttactttca attgtaacag gcttctcgtt
    25921 tacggcaacg gccacgggcg taggtttagc tcgtgatgtg gctttagcga ggctttgtgt
    25981 cttataaagc tggtcaacaa agccagagct gtcgagttct ttcatgaacg tcagatcaac
    26041 gaactgctca gggcgagcac ttcgggcggc tggttctttg acacccattt cccggagcat
    26101 gatttgaatt cctttaattg tcggataggg cttttctgga atgagcgagt taaggacagc
    26161 gtcataggct tcatccaggg cttctgtatc gtcggacctc agatacttgg ccaggattgc
    26221 tatcgtctcc tggcggcgtg tcttcatgaa gtgaatactc tcgacaaaag ctttcaagac
    26281 attccggaca agctccggtt gctttttgat agtatcttgg ctgaaggcta aaccggtatg
    26341 ctggtattcc aatccgagca tttgtagatc cgccagagtg ttcaggccca tccttttggc
    26401 tttagcggtt aatgggatcg cgatcataac gccatgtatt ttgccggttt ccaacgcgga
    26461 gaagcgggct ggttggctac cgatctgaac aatggcaaca tccttatcag gcgtcagtcc
    26521 atacttctct agcgcatagc gagttgcgaa atcggaagaa gagccaatgc gactgactgc
    26581 cattatcttg cctttgagct gatccgggcg ggtaatgtca cgcgacacga tcaacttgta
    26641 atccatggtg ttgagaaatc cggcaaccat gacgacaccg gatccttgca gattgctttg
    26701 aagcacagat gagcctgcca cctgagcggc caggacatcc cctgatataa gcgtctgaac
    26761 ggttcggctc cctccctcaa caaaaagcag ttgtgagtcc aggccatact tgcggaaaaa
    26821 tccctgatcc tgagcgatcc aagccggcgc ctggctgggt gaaatagcgc tatagccgat
    26881 catcactttt ctcagcggct gggttgcaac actttctgcc gagctcgcat cgcccaccac
    26941 cgcgccgaac ggtaacgcac acaaagcact tattgtaagc gctcgggtaa aggcctgttt
    27001 gatcaatcgc agcattttga atcctcctat ctgatagagt ccttcgcgat ctattcgcga
    27061 ttcaatcgga ccatggcggc tttgaggctc gctctcatgc gttcgagtga tcgcgccaac
    27121 tccccaacct catcagtaga ccgaattgta accggcgtat ccagctggcc gcggctaatc
    27181 tcatccgcgc ttgtcttcaa ctccattagc ggccgaatcg cctttgcggc gagcagtagt
    27241 gacaggacga taccaagcgc aagacacccg gcgatcaaag cgattatggt gaacaccgtg
    27301 ccacgaacgt ctgtctgaat agaatcggcc cagaggccga cgtgaacagt gccgagctga
    27361 ccttccagaa gaggaactcg agtatcgtaa atggatttgc cacgaatgtt cgtcaccctg
    27421 gaatgggccg tgcgacgttc caccgcgctt gaatctttta gctccgatgg gaagggctgc
    27481 agactcgtgg caataatatc tcccttaggg tcttgaatat aggcgtacgc gacaccatcg
    27541 agtcgaccat atttggcaat cagaacgtcc aattcaagcg tgttctgacg ggaaacataa
    27601 gccgctgatg cgtcgcttaa attcgtggcg attgccatcg cacgaaggtc aacttggcgg
    27661 tgcaaggcgc tatcagtcat ggtgtagacg atccctatga ccagcactcc gagcatgaga
    27721 atcaacccgc taaaagcgac gctgatcttc caccttaggc tggctccctg ccaggctatg
    27781 tttaacggct tgttgacttt cgtcgtctcg attgaaaggg attcttgtga cattcttcct
    27841 ccggatcgct tgcaggattg ctaaaaccgt ttgagagtgg agatctcttg aaaggcggat
    27901 tcttcgaact tcagcctgag ttttgcgtcg ccaatttcct tgccgacatg ttcaatcagg
    27961 tcgtggagtt tggattccgg aaattgggcc tgagtttcac caagtccttc gatttgatca
    28021 cgcacgacta aagaggccat gggccccatg atttccgtta aaagatctat gacgcgaccg
    28081 agtaccgatg cggggaccat ttcggttgac gggcgggacg atggggcgac aatttcaacc
    28141 aggcctgctt tttgtaggtt gaagattacc ttggccgtat acgcataggg gattcgaagc
    28201 gcttccgcga tctcctgcac ggagcggttg ccatcgatct tgctgagcac tcgccagcca
    28261 acatgcggaa cggttacggg accgttttgt tcttccccca aatcggctat acgaagaatg
    28321 gttttaccag acgggataac ttcgagaatg ttttcccatt cacgactcat tgcctttact
    28381 tcgtcgagga tttctgatga cgacttcttt atagttgcag aaggtggcgt tccagtcggc
    28441 agtaatgcga agtttccttc tccccacgta accggataga gaagcgcctt ttcgccgtcc
    28501 ccgatcgggc atgtggcgtg tataatgctg ccatcgacga gataaacctc tactgtctcc
    28561 ttacctcgag agagtttaag cacgccggac ttcttgccgc cggtagcaag catcaaaata
    28621 tcattgagac tgatttgtga gaggtttcct tgcatctctt ttccggtatt aggctgacgg
    28681 tctaacgatc ttccaaagta cggcgcccgc tcgtgctggt ctgagccttc tttacactct
    28741 ccaacacttc ctcgttgcta ttcagtgtaa ccttgagcgt ctttatcacg ccaaggctct
    28801 tgctgattcc gaccatctgt tccaatgtcg atttgatctg tgtttgaagg agatcgactt
    28861 cctcagtcga gatgcctagt aattgtgttt gcagctcatc ataaaggcgg gtgatgctgt
    28921 cgatctgacc cagcgattcg gttttctggg tgatctcttc atagaggcgg gaaatatcct
    28981 cggtgagatt ctgcaattcg tttttcataa atgttcggcc ttctttcctt atttttttag
    29041 cgtcggagac ttattgggcg tcttcccatc gttcgcttta ctcggtcgtt tcaaatcgcg
    29101 gattccggaa gtgctttcat cgcttgtagc agacacgggt tcagcggccg cggggacgct
    29161 cggtgcctct tgtgatttgg atgatgagaa gaaataaaaa ccagaagaaa gtgcaactag
    29221 cagcaaaaat acaccgagcg cggcatactt tacgttggtt cgattctgct gtgagtagat
    29281 atcatattgg ccataatctt tgataatttt ttttaactcg cgatcgatgt cgccttcctt
    29341 tttcaagggt aaggcccgag ctgtattgtt cgttgcactt tcaagggcca ccttctctgg
    29401 gaagatgggt ttgttaccga tctcattagt gtcggttctt gcttccgagt cgaccttttt
    29461 aggtgaatcg gacgagctaa agaattcgcc gtaattcttg atgagatcat ctaaaagccg
    29521 ctgatcgaaa tcgccgccga ttggaacaag cgccgtcgat attgatgtat tcgattcttc
    29581 cggcacctgc tcctggacga actcggtagt gcttacatcc cggtgcgtag ttttatcggg
    29641 ttctaatcct agtggcgtgg tctggatctc cggttcataa cgaaggttct ggatattatt
    29701 ttgatcgggg tctgagtcct tgtcccatga aaaatccgcc gagggaattc ctggggctgc
    29761 ttcgatttca gccgcgtcga agacgaatgc cggctcattc ggagattccg tcgattcgcg
    29821 gcgctctgca gccgaacgtg ctctgatcac ttcttttgcg gattttggcg tatccttcgc
    29881 ttcctgatgt atcggaccag cgctgggcgc gataggtaca gtactttcgg ggcgcggacg
    29941 caaggttcgt ttgaggtccg gaaatttgat cagattgctc gcttgaatga tgtcgcgcag
    30001 gtggatgggt ttgcccaagg catcgatggt gcttagattt atatcttccg attttgcttt
    30061 ttcgacggct tcgtagacca cagtcatgga ccgcttaatt gcgaccagag atccaacacg
    30121 ggactgcaag ttcacgagac cgcgcagcaa agtgcgtgac ttattttcgg cggcggcgat
    30181 ctcttccaac gaatagtcgt tcagccgctg ctgaagtccc tgaatcgaat taactacttt
    30241 accaactgct ccaccgttcg gcgaagttat ccgcggggac gtggacccgg ctcggtttcc
    30301 atcttgtttt gctgacaaca ttttaactcg gaagatacaa attggccttc gcttagtcgt
    30361 agagaataag ctatcgaaag caaaagagtt gccatgagcg tcaagggtaa aatgttcgta
    30421 gtttcacgta gatcgagaca gtctttccgc aaaattgcta gataactccc ccagttctgt
    30481 tcacctaaac ctaattgtgt acagggttgt tctctatgta cttagtgctc ttggaaagtc
    30541 cattcgacgt ttgtcggcag ggaatagaaa tgatgccaat accttctgat cagaaggagc
    30601 ggagtcggct cgttccttaa ctacaatgca gaatgattga catacacctt tttgtcgtac
    30661 gtcagttgca gttgtattga actcagaaag atagtgagcg tctatagggg actttcggat
    30721 gtggccggtt attcttgcaa gatctcgaac cgttcgattt ttcgatacag tgtgcgggtc
    30781 gagatgccga gcgcttcggc tgctttggcc ttattgccgt tcgtttggcg gagggcatcc
    30841 tcgatgagat tcctctcacc ttcctcgata ttcttgattg ccttcgttcc gatttcctgc
    30901 cgatgtcccg tcgcatagtg ctgcaggttc atcggcagat cggcaatcgt aatgtattga
    30961 ccatcggata ccattgcggc actttcgatg acgcttttga gttcccgaat gttgccgggc
    31021 catgggtaat ctaggaataa agccttagcc gaactgctca gcgtttttag cggcttgtga
    31081 aactgattac aagcttgata gacgaagttt gcaaccatca agatgatgtc ttctttacgg
    31141 gcccgcaaag gcgggagaat gatgcgagcg ctgttgaggc gaaaatacaa atcctttcga
    31201 aatccagttc cctgaatcgc ctcttcaagc tctttgttgg tggcggcaac gattctcaca
    31261 tcgcttaatt ttggtgttga ttcgccgact cgatagtact gacgtgtttc caggaagcgc
    31321 aaaagtttca attggatcgc cggcgcgagt tccccaattt catccaaaaa cagcgttccg
    31381 ccttccgcag cttcaatgag tccctttttg ttccgctccg caccggtaaa cgcgccacgc
    31441 acgtaaccga agagttcact ttcgacgagt gtctcaggaa tggcggtcat gttacaaccg
    31501 acaaacggtt tcgtcgccct tgaaccgttg gagtgtatat actgagccat gacttctttg
    31561 ccggtcccag tttcaccggt gatgagaacc gtgatgtcat gcatggcata tcgattcgcg
    31621 aggtttagca cctcatagaa ttgaggcgtt tcgccgatca tgagcggtgg cttgatagag
    31681 ttcggaacca gcgaatcgct tgtaagcggg ctgggttgac ccaaaagtgt ataaagcgag
    31741 tgagtattcc gcggatgata acaaaccagt gtaacctgcg tgccgaggcg gtaaatcgta
    31801 tccaagaacc tcggccatgt gttacctcga caaagcgtca ggtcggcgaa aacaaacttc
    31861 agttgctcaa tcggcaacgc gttgaaggcg tgctcggctt cctcgagtga gcgggtttcg
    31921 tgaatgaaac agtcccgtgc ctccaaaagt ttggccaacc cggagagaga agagtcgatt
    31981 gaataaatca gtagagccag ctgtcgcgac atcagatgac ttactcttaa gaagaagagg
    32041 gattgggcag aaccaccatg ttttctaatg agtcggtaag cagctggcgc gtaatacgcg
    32101 gctgagttcc catatagttg caggcagcga tgatgtgctc ggcgatgaat ttgggatgaa
    32161 atcctgcaaa gggaaccttg tgtttgatgt aaaatgaatt tagcagatat gcgatgatct
    32221 cctccgaaaa ctccagtccg tacgattcac agacacgttg aaatatctct ttgtactcat
    32281 ccgctgtcgg aggactgatc tttatcttgt aatgtacgcg gcgtagttgt gccgcatcca
    32341 taagttcttc cgggggaaaa ttggtagcaa agattacaag ttgatcgaac aatacggcaa
    32401 attttttacc ggtatgaagc gtcaagtaat cgagtttacg ctcgagagga aaaatccatc
    32461 ggttcaagag ttcatgaggc cggacacgct ggcgcccgaa gtcatcaata acgaaaattc
    32521 ctccaatcgc cttcatctga aggggcgcct catagtactt cgaatgaaca tcgaagtcca
    32581 aatcgagcat ggctaatgtc aattcacctc ccgagattat gtagggacgt ctacagcgaa
    32641 cccagcgagg atcataggga aggtgaggta aaaacatcgg atgcttgtca ctgctcacat
    32701 cgcgaggatc ggcgccaaat gggacgtgaa cagagggatc gaatattttg ataatctggc
    32761 catctgcttc tatagagtag gggatgtaaa cgggttgctc gaaggcgctt attagagctt
    32821 cggccatagc agttttgccg ttccctgcag ctccatagat caagatggct ctacccgaat
    32881 tggatgcagg accgagtctt ctgacgattt cttccgacaa aaccaagtga gacatcgact
    32941 ttttaagttt gtctatgctg acgatttcgt ttgtgatcgt ctgtttttgc acctgcagct
    33001 gataagtctg cagcggcacc gggattggcc ctatatattc gcaaagtcgt aatgcctcac
    33061 tagcgcgctc cttaccggca tttgtaaggg cgtaccgcat aatgttgtga ttcttctccg
    33121 aagggccacg aatttctaca agagcatctt ttttggctac ggttagcagt tcctcgacca
    33181 cacctcgggt taacttgacg tgttcggaaa gctccgggat tgtttgcaat gctgacacgt
    33241 acatcgtccg caaaaggcaa ttaagtataa aattgccttc caatccggtt tgttcggagg
    33301 tttttggaat cggcgggggc tcgtgaagga tggcgtccaa gttttcgcgg ctaatgtatc
    33361 caagcgaaat aagattatcg ccgaggcgcc caccggcgat tttttgttgc cgcaacactt
    33421 gctgaagctg actgtcgttt atgatgcctt ttgctctaag taattcgccg attgccatgt
    33481 attattctcg ttgtgtcaaa aaagattgcc tttgcgctaa tttccaacta tcttgttgat
    33541 atattcttca taaaatcgtt tcattataaa ttaagaagga accctgtcaa gcaaagttct
    33601 gcgcggcctt ggtaggcggt cagaggagcc ctcaaatgtg ttttttaggc gtacgatgtg
    33661 catgggtcag ttttgtcata gtaacgataa tactggtagg tggatgtgcg attcaggaaa
    33721 gagatacaaa aggacaactg tctgctgccg ggctaatcgc tacaccgccg gctcattata
    33781 gcactcagaa agcgcggtat ctgggagaaa aataccaaca gaaactcaat cgattgatcg
    33841 agatgattat cgcaaaccca aaaacgtcaa agctgcaatt tgcgaataat cttggatctg
    33901 ccggaggaat ggggttcttt acgcattctg cggtcaagac ccctgatgaa agatttcttg
    33961 aagttgtact tggaaccgga gaaaacattg atgtgggaga gtacagtggt aaagtcgcgc
    34021 ggctgttttc tgcatatggc cgagagttgt tgctcatcct tgccagtgat ctcgagattt
    34081 ataatgaccg ggagctgtcc ggctacggct tgaactttac ctggcggacc gcgggttccc
    34141 gcatcactac ggagcgtgcg attgtctact ttccaaaaga gaaggtaagg gcatttttga
    34201 aggaagaact aggcgagaat acgcttctag ctgaggcggt tattttcgtc atggagtctg
    34261 agggccaggc gaatcgcttg tcgttccgcg cgccagagcc cgctcctgat gtccgagcgc
    34321 cgattcagga acaggtcctt gcaccaaggg ccatcaagga aaaagcggat gtcgagccgc
    34381 tagttgcacg gggagatgcc aacagtacga aaaccatcga gccgggaact aatgcggacg
    34441 agacctcagc cagggagaag aagagaccta tcccaagttc tcaaatgcag actagcccaa
    34501 atccagtaac tccgcggcgg aatctagcga caaaggaaga gatcgtcccc gacccaaagg
    34561 ccggtgcgtt tgttggtgac aaagcggagc cggttggacg aggtcctgcc tcagaatcag
    34621 cgaccgaatc tgctggggat ctgatgcccg cggtgggcgt aatcgaaaga atggagac
//
LOCUS       DASUVS010000448         9989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_198855, whole genome shotgun sequence.
ACCESSION   DASUVS010000448 DASUVS010000000
VERSION     DASUVS010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9989
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198855"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..811)
                     /locus_tag="VF182_23585"
     CDS             complement(293..811)
                     /locus_tag="VF182_23585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440119.1"
                     /translation="MSVRLSGTTCAHDLTRVRRPLVAQRRFLAGIPGPLLTRKPPDVL
                     VVARGKLLELPPPKSLGEALDLLASGVGLVIRRAEKMDDGIAGLATSVTQHIPGKVHV
                     QLIVTPAGTYRFGWHYDDEHLFIVQTNGSKDYFFRDNTVERERPSGTGPDFTRFGHEN
                     SPMGTARLLAGD"
     gene            complement(974..1291)
                     /locus_tag="VF182_23590"
     CDS             complement(974..1291)
                     /locus_tag="VF182_23590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX6440120.1"
                     /translation="MGSKLYVGGLPYSVTEGRLQEVFSTHGTVESANVISDKFTGQSR
                     GFGFVEMSSGSEAQSAISALNGTQLDGRTLTVNEAKPMAKRDNFGGGGGRNDRDGGGR
                     NRW"
     gene            1905..2159
                     /locus_tag="VF182_23595"
     CDS             1905..2159
                     /locus_tag="VF182_23595"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX6440121.1"
                     /translation="MKYPLVSRKAGILIAMALELPGSIVGGLFIGYLLDNYFNTSPWI
                     MMILTPLGFTAACIRLVRWVKYFSEKRNETASADREQPAD"
     gene            2298..2942
                     /locus_tag="VF182_23600"
     CDS             2298..2942
                     /locus_tag="VF182_23600"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEX6440122.1"
                     /translation="MKRLCQPNVLRILSLTTVIGLTLIGGYARAQTGSAGNTISVNGT
                     ISSITGSDMTVMSNGGPVIVKLGDKTIIRGEEPVKFSDITSGMYVGATAAKQPDGTFR
                     ASRLHIFSEDQRGTGEGHRPLTSAQQSGATMTNANVETVEDVAVQNVKGRMLTLKYKG
                     GEIKVLVPADTLVVRRVVGDRRLLTVGSTVSASGARADDGAIVASQITVRASAN"
     gene            3282..4619
                     /gene="gdhA"
                     /locus_tag="VF182_23605"
     CDS             3282..4619
                     /gene="gdhA"
                     /locus_tag="VF182_23605"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEX6440123.1"
                     /translation="MREIEEVLNKVRATSEHQPIFLQAIREVFESIEPAVSRHPQYIK
                     HGILERMVEPERTIIFRVPWVDEQGKIRVNRGFRIEMNSAIGPYKGGLRFHPSVNSGI
                     LKFLAFEQTFKNALTTLPMGGGKGGADFDPKGKSDMEVMRFCQSFMTELFRHIGPDTD
                     VPAGDIGVGGREIGYLFGQYKRIKNEFSGALTGKGPNWGGSLIRPEATGYGAVYFAQE
                     MLNGRGDSLSGKVCAVSGSGNVAQYTIEKLNHLGAKPVTLSDSDGFIYDRNGITPEKL
                     AWVIGLKEVRRGRIREYAEHFKAEFFPGKRPWGVACDCAFPSATQNELDSDDARILLR
                     NGCGLISEGANMPSEPDAVRQFIESKICYGPGKAANAGGVAISGLEMSQNSLHYSWTR
                     QEVERRLQEIMHSIYAAASSTAAEYGQPANLVMGANIAGFVKVADAMIAQGVV"
     gene            complement(5133..6515)
                     /locus_tag="VF182_23610"
     CDS             complement(5133..6515)
                     /locus_tag="VF182_23610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6440124.1"
                     /translation="MATQSKEALRNLGGAIKGRVLTPDDSGYDEARQIWNAMIDRRPA
                     VIVQPADGDDVAPAIKFARENSLEISVRGAGHNIAGNAICNGGLMIDFSKMKQVQVDA
                     ANKRAYVDAGATLGDLDSATQRHGLATPVGINSTTGIAGLTLGGGFGWLTRKYGMTVD
                     NLVSVEVITADGKKLRSSARDNQELFWALRGGGGNFGVVTQFEFALHPVGPEIIAGLL
                     VFPFAQAKQVLTQYRQFAKAAPEDLSVWVVLRQAPPLPFLSQDVHGKEVIVLPIFYTG
                     AAAAGQKLIGELRAFGNPHGEHVGAQPYAEWQKAFDPLLTPGARNYWKSHNFTEISDG
                     AIDSIIEYAGKLPSPQCEIFIGHIAGAANRVASEATAYAHRDAKFVLNVHGRWESAAD
                     DRRCIDWARAFFKASAPYASAGAYVNFMTEDEGERVAAAYGSNYDRLAQLKKRFDPEN
                     VFHLNQNIKA"
     gene            complement(6800..7807)
                     /locus_tag="VF182_23615"
     CDS             complement(6800..7807)
                     /locus_tag="VF182_23615"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6440125.1"
                     /translation="MPEWKMIDADGHIREVESDVFEYLPDYYKQRREALLYFPLLPHH
                     GWHRQAGRRSGAGSSFLIPSLQDWRTALEQGNIEAAVVYPTRFMHIGQVGMAEFAVEL
                     SRAYNDYLYDRFLCDEPRLRGVAVLPLQDIDAAVAELRRAVRDYGMVGGVLPADGLPR
                     PLGHPEFHPLYEEANRLGCMLAVHSQNSLRNNDLFMWRDEAATLAHVWPQMRQFTNLM
                     FSGVLGKYPDLRIAFLEAGCGWVPYLISKMEGRLGELPRPLKLIERGQVYFQCGEELT
                     TGRDLELLGDHCLFWASDFPHEGIVDMSKAVEEFLSRDDIPASAKKKISYDNAKKLYG
                     L"
     gene            complement(7917..8933)
                     /locus_tag="VF182_23620"
     CDS             complement(7917..8933)
                     /locus_tag="VF182_23620"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6440126.1"
                     /translation="MANYKVLDADGHVRESVAGMREFLEPRWHRRNLFPNDEWDRDLH
                     GKLGQKPEGPEDQLAAMDEDGIDVMVLYPTAGLHVSALHEKDYATGLTRAYNDWLFHF
                     CKKDSTRLKFVALLAPQDPAAAAEELKRAVTERGALAGVLPTYLPQMPDFGDDFYDPI
                     YATAQQLGVGIGFHTGTSADSLGGQRFRKFISAHTIDHPAEQMMALIATIIGGVFERF
                     PRLSIGFLEAGIGWVPYMMDRLDEEVEKRGAVEAPYLTKLPSEYVKSGRIYFGVECEE
                     KTIPDAMHWGLEDTILYSSDYPHWDGDWPHTVKAIRDRKDLADTTKRKMLHDNVARFY
                     RFNE"
     gene            complement(9025..9267)
                     /locus_tag="VF182_23625"
     CDS             complement(9025..9267)
                     /locus_tag="VF182_23625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440127.1"
                     /translation="MTARLRLPDDARAEVGYQDLIRGVEPKPYPSLEGLRNLQRLMRV
                     HNPKVGSFRVEEMVDDRILRKIIEGGFLDNVRIANR"
     gene            complement(9273..9611)
                     /locus_tag="VF182_23630"
     CDS             complement(9273..9611)
                     /locus_tag="VF182_23630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440128.1"
                     /translation="MGAILGLEYLGLEPSGDRISILVVGDQTNTGQALEAGSIDAALL
                     DGVFTRRLRQKGIPVIAEFAGANIPFAGQGMVTSGLYLIKQAAVIENFLKGLLEGIDF
                     SLAPATRRRC"
     gene            complement(9596..9880)
                     /locus_tag="VF182_23635"
     CDS             complement(9596..9880)
                     /locus_tag="VF182_23635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440129.1"
                     /translation="MRKNGLEVLLVLTRVSQPAIAALLAGEMQMVYGGSTAALGAISS
                     GADLKVIAALTNRLTYDLVARPGINRAEELCGKPLGVGSIGGHLSGWVRY"
BASE COUNT         2320 a         2799 c         2635 g         2235 t
ORIGIN      
        1 tctctgttcc tccgccccgt atcggccggc cgcagcttct tctcatcagc cggcgcgacc
       61 atatttagta acaatcctgt tcatcacgcc tacacaccga tccagtcacg cgaagcggct
      121 ggctcgctgt ccgaaaccca tacggtgcag tacacttagg gtcatccgta tggtcgatgt
      181 tctcatttat cgttactggc aagccacaac gagtcggggg aaatgcctag taaaatcgat
      241 agtgaatcct cgatgcattt tgccgcatgc caccccgcgc gggaatatat aactaatcac
      301 ctgccagcag ccgagccgtg cccatcggcg aattttcatg gccgaatcga gtgaaatccg
      361 gcccagttcc cgacggccgt tcacgctcga ccgtattgtc cctaaaaaaa tagtccttcg
      421 aaccattggt ctgtacgatg aaaaggtgtt catcgtcata atgccagcca aacctataag
      481 tgccggccgg tgtaacaatt aattgaacat gcactttacc gggaatgtgc tgggtgactg
      541 acgtcgcaag tccggcgatg ccgtcgtcca ttttctccgc gcgacgaatc accagcccaa
      601 ctccgctagc cagcaggtcg agtgcctcgc ctaaactctt cggcggaggt agctccagca
      661 acttaccccg agccacaacc aatacgtcgg gcggcttgcg ggttaacagt ggtccaggta
      721 tcccagccaa aaatcggcgt tgcgcgacta gcggacgacg gactcgcgta aggtcgtgcg
      781 cacaggtagt cccggataaa cgaactgaca ctcatccgcc tcagccactg atcgagcata
      841 gtctttattt aacaatttga atcttgccga gcgagcctac ctgttagagt ggacaatact
      901 gtttcgaaaa aggaagaggg gagcaacccg atctgttgct cccctcttct gtgttactta
      961 tttcagctaa agactaccag cgattacgtc caccgccgtc tcggtcatta cggcctccgc
     1021 cgccaccgaa gttatcccgc ttcgccatcg gcttggcctc gttgaccgtc agagtacggc
     1081 catcgagttg tgtcccgttt agcgccgaaa tggcactttg ggcctcactg cccgagctca
     1141 tttcgacaaa cccgaagccg cgtgattgac cggtaaactt atccgagatg acgtttgccg
     1201 attcgaccgt gccgtgcgtc gagaagactt cctgtaaacg cccttccgta accgaataag
     1261 gcaaaccacc gacatataat ttactaccca tgttctcctc ctatgagaga ctgttattgt
     1321 ctttgaccct gagaatccga ctgcaaatgg gaagaacatg gagcaacaaa atacttaaac
     1381 cacgccctga tcggatatca tttaaagaaa gcttcgcaag gatagagact ggaagaggcc
     1441 ttcaaatttg tgctttcccc agaaggccat agactgaaga agatacatca cactacgtgc
     1501 tgcctggagc aatgtcaacg tccatctctg atatttttaa aataagtgtg ctgttctagt
     1561 taagtgagct ggtcgtagtt gtaccaagca tattaggcat cgagcatacg cgcgtaaatc
     1621 ctcacgtcag agttcggcag atgtttcgtt tacccgggat taagttggat tgaagcggaa
     1681 tcgatttcga ctggttggat gagatcaata gttcctgcag ttagtgccca tgatctcacg
     1741 aaggtgatag acttcacggc gtggtctacc aacctaaatg gcatcaggct caccgtaccg
     1801 tgcgcctggc ttgttcctga cgacggggaa ctgctaatct cgatttgctt atggctggtt
     1861 cgaacaaacg cctatactcg cgccgccagg aaaaaactgg cgggatgaag tatcccctcg
     1921 tcagtcgcaa agccggtatt ctgatagcga tggccctgga gcttccggga agtattgtcg
     1981 gtgggttgtt catcggttat ctgcttgata actatttcaa tacttctccg tggatcatga
     2041 tgatactgac gccccttggc tttacggcag catgcatacg gctagtgcgg tgggtcaagt
     2101 atttttccga gaagcgtaac gaaactgcat cggcggaccg cgagcaacct gctgattgag
     2161 acgcgcgaac ggcgaagaat gaacggttta agaactctga tcatcaatgc aatgatctac
     2221 cgtgcaatac gttgcgcgga aatcacgaaa ttgacgacaa tgtggaacga aacgggatag
     2281 gattgaaagg aaaagcgatg aaaagattgt gtcaaccgaa tgttcttcgc attttgtcgt
     2341 tgacaacagt gatcggtcta acgctgatcg gcggctatgc acgcgctcag accggctcgg
     2401 ccggaaacac gatttcggtg aatggcacga tatcttcgat cacaggttcg gatatgacgg
     2461 tgatgtccaa tggcggtccc gtcatcgtga agctcggcga taagacgatt attcgtggcg
     2521 aagagccggt gaagttttcc gatattacct caggtatgta tgtcggggcc accgcggcaa
     2581 agcagcccga tggcactttc cgcgcgagcc gcttgcatat tttctccgag gaccagcgcg
     2641 gcacgggaga aggccaccgg ccgttaacct ccgcgcagca gagcggcgcg accatgacca
     2701 atgccaacgt tgaaactgtc gaagatgtgg cggtgcaaaa cgtcaaaggc cggatgctaa
     2761 cgctgaagta caagggcgga gagatcaagg tcctggtgcc ggcggacacg ctcgtcgtaa
     2821 ggcgtgttgt cggcgaccgg cgcctcctta cagtcgggag cactgtgtca gccagcggcg
     2881 ctcgagccga cgacggagct attgtcgcct cgcagattac cgtgcgcgct tcggccaatt
     2941 agctcgcaag agacattaga tgttagatcc gaatgcacgg ttgaatactc ccggttcaaa
     3001 ggctctagta agcctcttat tccgaatagc cttggaacgc gctcagtttt gtctaagtca
     3061 gacggcctaa catcccttcc gctcggccga gctaatctcg cggcgcggta tcatcttatc
     3121 tgctctcttc cttcctcata gaatcagaga ctccatccgt tcccatcttt gcgccgccag
     3181 tagaatccca tgcggttttt ggttagctaa aataaccgcg aaagagctaa ccggctcatt
     3241 gtaggcggcg atccgacaca gtaggaggca ccttttagat gatgcgcgag attgaagagg
     3301 ttttgaacaa agtccgggcc acgagcgaac atcagccgat ttttttgcag gcgatccggg
     3361 aagtgtttga atccatcgag ccggcggtga gccggcatcc tcagtacatc aagcatggta
     3421 tcttggaacg catggtcgag ccggagcgga cgatcatctt ccgagtgccc tgggttgacg
     3481 agcagggtaa gataagggtg aatcgcggct ttcgcatcga aatgaacagc gccatcggcc
     3541 cgtacaaagg cggcctgaga tttcatccaa gcgtcaattc cgggatcctg aagtttcttg
     3601 ctttcgaaca aacgttcaaa aacgcgttaa caaccttgcc aatgggcggc ggcaaaggcg
     3661 gcgcggattt cgatcccaag ggaaaaagcg acatggaagt gatgcgattt tgccagtcct
     3721 tcatgaccga gctatttcgc cacattggtc cggatacaga tgttccggcc ggggacatcg
     3781 gcgtcggcgg ccgcgagatt ggttatctgt tcggtcaata caaacgcatc aaaaatgaat
     3841 tctctggcgc gctaaccggc aaggggccca actggggagg ctcgttgata cgtcccgagg
     3901 ccaccggcta tggcgcggtt tactttgccc aggagatgct caatgggcgc ggcgacagtc
     3961 tcagcgggaa ggtttgcgct gtttccggtt caggcaacgt ggcgcagtat accatcgaga
     4021 aactcaatca tctcggcgct aaacccgtca ccctttccga ctcggatggt tttatttatg
     4081 accgtaacgg catcacaccg gaaaaactcg cctgggtgat tgggctgaaa gaagtgcgcc
     4141 gcggccgcat acgtgagtat gccgaacatt tcaaggcaga gttctttcca ggaaaacggc
     4201 cctggggcgt cgcgtgcgat tgcgccttcc ccagcgcgac gcaaaatgag ctggacagcg
     4261 atgatgcgag aatcttgctc cgaaacggct gcggtctgat ttcagaaggc gccaacatgc
     4321 cttctgagcc cgatgccgtg cgtcaattca tcgaaagtaa aatctgctac ggtcccggca
     4381 aagcagccaa tgccggaggc gtggcgatct cgggactgga gatgagtcaa aactctcttc
     4441 actacagctg gacacgacag gaagtcgagc gccgcttgca ggagatcatg cacagcatct
     4501 atgcggcagc ttcgagcacc gcggccgagt acgggcagcc ggccaatctg gtgatggggg
     4561 ccaacatcgc cggttttgtc aaagtcgctg atgcgatgat cgcgcagggc gttgtgtagg
     4621 gtgatcaatt ttttgaatca ggaaacttca cccatctgtt ttttgcattt gatcgttaaa
     4681 acctgacgtt caccccgacc gacgatgttc gtgccagcgt acgcagagat tcattacgtg
     4741 attcgcctgc cttctctgta ctgaaaagaa cacacttagc gcgaggaaac gggccggaac
     4801 tctaaaaatc aggacagatc tgggcccgtg ccgtcgtaga cgtgctggca cgttaactgc
     4861 ctcctacgag ggcggaggta attagccatg gctgaagaat cctgtgccca tcctaattgc
     4921 gattgcgaag ttgagtccgg cgccggcgta tccaaaggcg gaaagattat tgcagcgagt
     4981 tctgcgcggc cagcgaagac gcaggtctgg aagactgcga gtgcggccat tcagattgcg
     5041 agtgatcaga ccgcgttaag ctaattcgag cttgacctga ccggcgcggt taacagccgc
     5101 aataaattat caagtgaacg cgcgacgata ttttaggctt tgatattctg attcaaatga
     5161 aaaacattct ccggatcgaa gcgttttttt aattgtgcca gacgatcgta gtttgatccg
     5221 tacgcggcgg ccactcgctc tccttcatcc tcggtcataa aatttacgta agcgcctgcg
     5281 gaagcataag gcgcgcttgc tttgaaaaaa gcgcgtgccc agtcgatgca cctccgatcg
     5341 tctgccgcgc tttcccaacg gccgtgcacg ttcaaaacaa atttagcgtc tcggtgcgcg
     5401 taggcggtag cctcggacgc gacccgattg gcggcgccgg cgatatgacc gatgaaaatc
     5461 tcgcactgcg gagagggcag tttgccggca tactcgatta tcgaatcaat ggcgccgtcg
     5521 ctgatctccg tgaagttgtg cgatttccaa taatttcgcg cgcccggagt gagcagagga
     5581 tcgaaagctt tttgccattc tgcatagggc tgggctccga catgctcccc gtggggattt
     5641 ccgaaggcac gaagctcgcc gatcagcttc tggcctgcgg cggcggcgcc ggtgtaaaaa
     5701 atcggcaaaa caatgacctc tttgccgtga acatcctgcg aaagaaaagg gagcggcggc
     5761 gcttgtcgta gtacaaccca cacactgagg tcttccggcg ctgccttcgc aaactgtcga
     5821 tactgagtca ggacctgctt cgcctgcgcg aacgggaata caagcaagcc ggctatgatt
     5881 tcaggaccca ctggatgaag agcaaattcg aattgagtaa cgaccccgaa gttgccacca
     5941 cccccgcgca atgcccagaa tagctcttga ttatcccggg cgctggacct gagcttttta
     6001 ccgtccgcgg ttatcacctc aaccgaaacc agattgtcta cggtcatgcc atacttgcga
     6061 gttaaccagc cgaaaccgcc tcccagggtc aacccggcaa tcccggttgt cgagttgatg
     6121 cccaccggcg ttgccaaacc gtgccgctgc gtcgccgaat ccagatcacc gagagtcgcg
     6181 ccggcatcga cgtaagcccg tttgttcgcg gcatccactt ggacctgctt catctttgag
     6241 aaatcgatca tcaggccgcc gttgcagatg gcattaccgg cgatattatg tccggcacct
     6301 cgaacggaaa tctcaagcga gttctcccgc gcaaatttga tggccggcgc gacatcgtcg
     6361 ccatctgccg gctgcacgat aacggccgga cggcgatcga tcatagcatt ccaaatttga
     6421 cgcgcctcgt cgtaaccgga atcgtccgga gttaagaccc ggcctttgat cgcgccgccg
     6481 agatttctta gcgcctcttt tgattgcgtc gccataacgt ttacctccga ttttattatc
     6541 gattagttat tttcaactgt gacaactcca acctgagttg gatgacgtaa aagaaaaccc
     6601 acagccagtg tgtgtgcgga tggaaacaaa ctcgtagatg tcctccctcg cctattacct
     6661 tcactcattt tagtcaagat aattcggaga gaaatcggcc ggaaagaatt gggaaaccga
     6721 cagcgacgtc aagacgcagg aacaaattcg tcacacgagc gactgtggcg agattggctg
     6781 gcagcgccac aggtcgcatt tataaaccat acaatttctt ggcgttgtcg tagctgatct
     6841 ttttcttggc cgaggcaggt atatcgtcgc gggagagaaa ttcctcaacc gccttgctca
     6901 tatcgacgat tccttcgtga ggaaaatcgg aagcccagaa aagacagtga tcgccgagca
     6961 gttctaagtc gcggccggtg gtcagctctt cgccgcactg aaaataaact tgcccgcgtt
     7021 cgatcagttt caatggccgc ggcagctcgc ccaaccgccc ttccatcttg ctgatcagat
     7081 acggcaccca accgcaacct gcctccaaaa atgctatgcg caggtcgggg tattttccga
     7141 gcacgcctga aaacatcaga ttggtaaact ggcgcatctg cggccagacg tgggccaagg
     7201 tcgccgcttc atcgcgccac ataaagagat cgttgttgcg cagcgaattt tgtgagtgaa
     7261 ccgccagcat gcagcccaag cggttggcct cttcgtacag cgggtgaaac tccggatgac
     7321 ccagtggccg cggcaatcca tccgccggaa gcaccccgcc caccatccca tagtctcgga
     7381 ccgcccggcg caattccgcg accgccgcgt caatatcctg caggggtaga acggcaaccc
     7441 ccctcaagcg cggttcatca cacaaaaagc gatcataaag atagtcgttg taagcgcggc
     7501 tcagttcgac agcaaattcg gccatgccca cctgtccgat gtgcatgaag cgggtcggat
     7561 aaactactgc cgcctcgatg ttcccctgtt ctaaagccgt tcgccaatcc tgcagcgaag
     7621 gaatcaggaa tgacgatccg gcgccggatc gtcggcccgc ctgacgatgc cagccgtgat
     7681 gcggcaacag aggaaagtaa agtagcgcct cgcggcgctg cttataataa tccggcaagt
     7741 actcaaagac atcgctttcg acttcacgga tgtgtccatc tgcgtcgatc atcttccact
     7801 caggcatgta cagctctctt tctcttcatt ctcggccatt agttaatagt acctgcagta
     7861 cgagtttttg gagacatcgc accactccgt agggtgcgac gtttcctctt tcaggatcac
     7921 tcgttaaatc tgtaaaaccg ggcgacgttg tcgtgcagca tttttcgttt ggtagtatca
     7981 gcgaggtctt ttcgatcacg aatcgctttg accgtgtgcg gccagtcgcc atcccagtgc
     8041 ggatagtcgg aggaatagag aatcgtatct tccaatcccc aatgcatcgc atcgggaatt
     8101 gtcttctctt cgcactcgac gccaaaataa atccgcccgc tcttcacata ctcactcggc
     8161 agcttggtta gatacggcgc ttcgacggcg ccacgctttt ccacttcttc gtcgagccgg
     8221 tccatcatgt agggcaccca gccgataccg gcttcgagaa aaccgatgct gagtctggga
     8281 aagcgctcga acacgccacc gataatggtc gcgatcagcg ccatcatctg ctcagccgga
     8341 tgatcgatgg tgtgcgcaga gataaactta cgaaagcgct ggccgccgag ggagtccgcg
     8401 gatgtgccgg tgtgaaagcc gatgccgacg ccaagctgct gcgcggtggc gtaaatcgga
     8461 tcgtaaaagt catcgccaaa gtcgggcatc tgcggtaggt aggtcggcag cacgccagcc
     8521 agcgcgccgc gttctgtaac cgcgcgctta agctcttcgg cggcggcggc tgggtcttgc
     8581 ggcgccagca gcgcgacgaa tttcagccgg gtcgaatctt tcttgcagaa atgaaacagc
     8641 cagtcgttgt aggcgcgcgt gagtcccgtc gcgtaatctt tttcgtgcag cgcgctcacg
     8701 tgcaaacctg ccgtcggata aagaaccatg acgtcaatgc catcttcgtc catagcggcc
     8761 agttgatctt ccggtccttc cggtttttgt ccaagcttgc catgcagatc gcggtcccac
     8821 tcgtcgttgg gaaacaaatt gcggcgatgc cagcgcggtt ccaggaactc cctcatgccg
     8881 gccacagact cgcgcacgtg gccatccgca tcaagaactt tgtagttagc catagcctaa
     8941 tcctttctct gatgttggtt tcattgaacg cacccccatc cgcacgcctg caaccctgag
     9001 ataaggggta gcttctcctt ctatctaacg attcgcaatc cgaacattgt caagaaagcc
     9061 gccttcgata atttttcgca gaattcgatc atcgaccatc tcctccactc taaagcttcc
     9121 cactttggga ttgtgcactc gcatcaagcg ttgaagatta cgcagcccct ccaacgaagg
     9181 atacggtttg ggttcgacgc cgcgaatcag atcctgataa ccgacctcag ccctggcgtc
     9241 atctggaaga cgaaggcgcg ccgtcattgt ttttagcacc gacgccttgt tgccggagcc
     9301 aagctaaagt cgatgccctc cagcaaaccc ttaaggaaat tctcgattac cgcggcttgt
     9361 tttatgaggt ataaaccaga cgtcaccatt ccctgcccgg caaagggaat gttcgcgccg
     9421 gcaaactcgg cgataacggg aatacccttt tgtctgagtc gccgcgtgaa aaccccatcc
     9481 aaaagagcgg catcgatgct ccctgcttca agagcttggc cggtgttagt ctgatctccg
     9541 acaactagaa tcgagatccg atctcccgac ggctcaagac ccaggtactc gagtcctagt
     9601 atcgcaccca tccagacagg tgcccgccga tactgcccac tccaagcggc tttccgcaga
     9661 gttcctcggc ccggttgatg cctggtcttg caaccaagtc ataggtcaaa cggttcgtca
     9721 gtgccgcgat cactttgaga tccgcaccgc tggaaatagc accgagtgcg gcggtcgacc
     9781 caccgtagac catctgcatt tctcccgcca gtaatgcggc gatcgcgggt tgcgacactc
     9841 gagtcagcac cagcagcacc tcgaggccat tttttcgcaa agaatccccg ttcctgggcg
     9901 atcccacaag ggagttacgc gagaattcaa agtggaaaga ccgacataga tcttcgtctg
     9961 cggttgcgct gcgccggctt taaaccaaa
//
LOCUS       DASUVS010000449        11885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_637305, whole genome shotgun sequence.
ACCESSION   DASUVS010000449 DASUVS010000000
VERSION     DASUVS010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11885
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637305"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VF182_23640"
     CDS             complement(<1..933)
                     /locus_tag="VF182_23640"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6440130.1"
                     /translation="MPVESNSLESGALTGLRILDFTSRMGGYCGLLLANLGAEVILIE
                     PAGGDAMRREGPFKDENPDPEGSISFAAYHTNKRGIVLDLESDDDRAKLRRLLGHADA
                     LIEDRPAGFWQRLGLGYEDLRAINPALVLTSISGFGSTGPYRDFKAPNIVAFAMGGLM
                     NLCGDPKRAPLSGPCDLAYRLGSVHAAFGTLVALFNRGVSGPSNHLDISLQDVLVADP
                     FLRIITRYSVTGEIPQRTGHSQATTVAETYQCKDGFVRIFCNQPDHWRRLVEWLGNPP
                     ELIDPKFENVQNRLPLRPLLDRLIELRTLDYHVKS"
     gene            1200..1589
                     /locus_tag="VF182_23645"
     CDS             1200..1589
                     /locus_tag="VF182_23645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEX6440131.1"
                     /translation="MNPAQTVYPLLADLVVLVHLGFVVFVGLGGLLMMKWPGLIWIHL
                     PAVFWGIVIELSGWICPLTPLENWLRRKGGEENYQFDFVAHYLLPMLYPQGLTRRSQI
                     LLGVLVLLVNVAIYGWVRGKQKGSTVQ"
     gene            complement(1743..3248)
                     /locus_tag="VF182_23650"
     CDS             complement(1743..3248)
                     /locus_tag="VF182_23650"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX6440132.1"
                     /translation="MNHSLKSYGSLTAAISLAVGFLFLTAPLGAQDTAPSTPTSKVER
                     KNEAPVSSEVLRVKLPKPIEAKLKNGLTVLIAEDHRFPAVSVQLQISAAGALYEPENV
                     AGLASVTAQMLREGTKSRDSKQIAEEADKLGASFGGSAEFGSSVTTFSASGLSDNFDT
                     WFPLALEVLLHPSFPARELDNLKQRLSVQLRQQRAAPGFLASERFNRALFGNHPAAVV
                     TATAESLESLTPAVLAKWHRERYVPQNAILGFAGNVRAGELIPKLEKWLADWEATSLK
                     ETLPANAVPTTERKVYLVDRPNSVQTTVALGNIAIDRRSPDYVPLVVMNHILGGGAAA
                     RLFLNLREEKGYTYGVYSDLSALRYPGPWRAGGSMRTEVTAPAVAEFFNEIRRIRDEK
                     VSDSELADAKRAITARFALSLEQPTGVLAFAMVQKTYGFPPDYWDNYPGQIMAVTAED
                     LQRVARTYLDPGTMQLVAVGDADKIKPALEKYGPIEIYDSSGKLAPVSKTD"
     gene            complement(3250..4650)
                     /locus_tag="VF182_23655"
     CDS             complement(3250..4650)
                     /locus_tag="VF182_23655"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX6440133.1"
                     /translation="MSKAISYPLALLILSLVAAGAIEQSQLTPAHAAAQPADTIAVPF
                     KEYKLANGLRIILSEDQHAPTYSICVTYNVGSRDEKPGRTGFAHLFEHMMFQGSANVG
                     KGEHFILVLNNGGSANGTTNADRTNYFQTLPANQLELGLFLEADRMRSLNINQANLDN
                     QRHTVQEERRQGVDNRPYGRTYEAVIGTAYDNFAYKHSTIGSMEDLNSATLKDVAEFF
                     RTYYAPNNAVLSLVGAFKTEDAMALIKKYFENIPSQAPPPVPDATEPEQQAERRKTVE
                     DPFAQTPRLDIVYKVPPGNTPDSYALQVLGEVLSGGLSSRLYQKFVKDQELALSISAG
                     PDERRGPSLFWVSVMPRPGINLDEIEKLVYQELERAKTEAVMDWEIEKVRTQVRRQRS
                     QSFYSTRGRANSLGHYAVYYNDPGLINSVQEKIFKVSKDDLQRVANIYLKESNRTVVT
                     TLPKAKAAAPTATIHR"
     gene            4899..5951
                     /locus_tag="VF182_23660"
     CDS             4899..5951
                     /locus_tag="VF182_23660"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6440134.1"
                     /translation="MASPVKEFEVHPYVQFMMDRYSPYRKWIESEGVPIIGGAFVKDV
                     RTEKLEYWKRKECKGAICAFSDQMVADAYIAEVDPGKSTKPQRQLYEEIITVAAGRGA
                     TTVWYEGTAKRVFEWERGSTFAIPLNAWHQHHNASGSEPCRYFALTSQPVAFELFRDP
                     EFIFNTSYMFKDRFDPGDPEFFSKEGQYFTEYYGGILHSNFIPDIRKINLVPREKRGK
                     GTRNMYIHMSGSAMLAHVSQFPVGRYKKAHRHGPGAHVFLLDSTGYTLMWNEGEKPQR
                     YDWHEATCISPPAGTWHQHYNTGQEPCKFVALHANTAVQREERGVEQIEFEDEDDSMR
                     KLYEEECAKNGVRMDM"
     gene            6011..7081
                     /locus_tag="VF182_23665"
     CDS             6011..7081
                     /locus_tag="VF182_23665"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6440135.1"
                     /translation="MNQLRLASVFLIAYLVLALVNVFGASEARAASASNPTRESESQN
                     ESIDELYEKAKKEGGQLTLYIALSARSEEIILPLFKKRFPAIAVNHIDATSDKLVARI
                     LAEQRGGRIIGDVFGGTPGYLAQMTEQKLLAPLALPEAAAYPAMLKGAEWVATDTQYF
                     IAGWNTSLVQKVDEPKQLEDFADPKWKGKLIAEPRDFQLLMGLAKRKYKSDEKAAELL
                     KRIAGNGVEFHKGHSQLIELLVAGQAPVCLTCYSHHFPPRMKKGAPILPLLSEGVGEV
                     GGSVAILKGAPHPLAARLWARWAVSDEGQRAYAQAGETPAHPNVEPAEKVRPASIHML
                     TADEVKEFPKYEKLWKGIFQIR"
     gene            complement(7082..8077)
                     /locus_tag="VF182_23670"
     CDS             complement(7082..8077)
                     /locus_tag="VF182_23670"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6440136.1"
                     /translation="MEQKGILYLSNEDVKRVLDLGRAIEITEQALRDHSEGRVIWSTP
                     EDLAIKPEQGWQSWVTGCALSTEPVAGFRIRSIKAAGGSRDVSRAPRGPRRILILSDR
                     EGGEIRAIMDEDWCHAVRTAAAATVAMRLLARKNAAILAMLGAGDTARAAVPVMAEAF
                     QLKEIRVTSRTPQSRQNYAREIGAEFGLNIRPVESTEEALSGADLVVSATTTSTPFVK
                     DNWLGEGITVYSIGKNQEMESEFYKRADKFVVDSWAHCKNKSDMQRLLKEEFISEKDL
                     YAELPELLSGKKPTRQSEKERIFIRAIGLVNQDVALANHIYHCAAAKRIGTVLPF"
     gene            complement(8256..9392)
                     /locus_tag="VF182_23675"
     CDS             complement(8256..9392)
                     /locus_tag="VF182_23675"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX6440137.1"
                     /translation="MATEAPFADEEYLTRYRRAQTLMERDGLDALVVSEKNNYWYFSG
                     LISYQLDHIQRPQICVLPKTGKPLLLVYGNDKAKAKALPWIGEVRAYTDVPFPEEMIG
                     DCLKELGLGESKLGFELGDDQRLGFPVNYLSRLTEALPKAQIKDGTAALTEMRLIKSP
                     KEIDFMRTACAISVKAYDRCLPQLRPGMTRREVADRLYISMIEEGAHPRHPGFLMLNS
                     STRYDDRRYNQGDRMIADFGACYEGYYGDVTRMTIFGEASAEHKQDHETACDVIDLCF
                     ESMQPGTPIAEVSRVANRELVKRGYQAVESPKRIGHGIGMSRAEPPSLNEVETELYRP
                     GMVLALEPKVRSEKSAVHLEEDVLITEKGPVFLTTGCRKLDVIK"
     gene            complement(9429..10136)
                     /locus_tag="VF182_23680"
     CDS             complement(9429..10136)
                     /locus_tag="VF182_23680"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX6440138.1"
                     /translation="MREWEQIIPEEERKIYEKAGYKGKDSFGRNPALLVIDVITGFTG
                     TKPMHVLDAIDEFPTSCGQVAWDALPNIQQLLHACRDADVPVIYSTSDPDFKAAFGNA
                     TKRGVDATDFEKLAVAFPEMIKPEKGEFVVHKARASAFFGTHLITYLVRKNIDCLLVT
                     GTSTCGCVRGTVLDGYSYGYPVFVVEECVFDRARTSHLVNLFEMNAKYATVIQLTEAL
                     DYVGKLKRVEGRKAFAS"
     gene            complement(10304..11302)
                     /locus_tag="VF182_23685"
     CDS             complement(10304..11302)
                     /locus_tag="VF182_23685"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6440139.1"
                     /translation="MFTRPLWILVVWLCSVPFALATEPSVIEGAKKEGALVFYTTMDI
                     QNSKPLLDAFTKKYPFIRGDLVRLGGTAMVSRIMTEAQAGASKFDVALGISPSLTPMR
                     ERNLIAPYVTSEAANLYDDLYDPKGFWTTIYLNTLVLGYNTKAISRNDLPKTYDDLLK
                     PEYKQKFIIDIENHDVFVALSQEWGQEKALNYFRGLAKQEPVFLRGNTNRANFVSIGE
                     RSMTFVYAQVIERMKQSGAPVDWIPLEPVAVELNIAMMSAKAAHPNSSKLFIDYLVSK
                     DGQEFLKTFRRIGPRKDVKPDPPKLFEGFRRRVVTPENYKNLREITRLHNEALGVR"
     gene            complement(11354..>11885)
                     /locus_tag="VF182_23690"
     CDS             complement(11354..>11885)
                     /locus_tag="VF182_23690"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440140.1"
                     /translation="FAREMSASTGIEVLPVTSAEEAVRGSDIVSAATNTVDPVVQGRW
                     LEDGMHITSLVGGDGFLPRKELDDDVITRAALIVVGYKPQIFIDRQAEFHERLERGLV
                     KAEDLHELGDLLNGKCRRRIDDKEITLFKNNTGMGIQFAATARKMYEKAREKGIGSEL
                     PLDLFMTRRGDKTYSP"
BASE COUNT         2617 a         3240 c         3264 g         2764 t
ORIGIN      
        1 acttttcaca tgataatcca gagtgcgcaa ctcgatgagc cgatcgagga gcggccgcaa
       61 aggcaatcgg ttctgcacgt tctcgaattt cggatcgatc aactccggtg gatttccgag
      121 ccattcaacc agacgtcgcc aatgatccgg ttgattgcaa aagatgcgaa caaaaccgtc
      181 tttgcattga taagtttcag cgaccgttgt tgcctggctg tgtcccgtgc gctgcggtat
      241 ttcacctgtg acactgtagc gcgtgatgat gcggagaaag ggatcggcaa caagtacgtc
      301 ctgcagcgaa atatccagat gattgctagg gccagagaca ccgcggttga atagggctac
      361 cagtgtccca aatgccgcgt gcacggatcc caaacgatag gccagatcgc acgggccgct
      421 gagaggggcg cgcttgggat caccgcataa gttcatcaaa ccgcccatag caaatgcgac
      481 gatgttcggg gccttgaaat cgcgataagg gcccgtgctg ccaaagccgc tgatggacgt
      541 caggaccagc gctgggttga tggctcgcag atcttcgtaa ccaagaccga gccgttgcca
      601 aaatcccgct ggccgatctt cgatgagcgc gtcggcatgg ccaaggagac ggcgcagttt
      661 tgcacggtca tcatcggatt ctagatctag aacaatgcct cgtttgtttg tgtgataggc
      721 ggcaaaggag atgcttcctt cgggatccgg attctcatcc ttgaacggtc cttcacgccg
      781 catggcgtcc ccgccggctg gttcaattag aattacctcg gcgccaagat ttgccagaag
      841 cagaccgcag taacctccca ttcggctggt aaaatccaag atacgcagac cggttagcgc
      901 gcccgattcc aatgaattcg attcgacagg cattcgatag atcctcgaga agtcgcaaat
      961 agatttttca aaactcacag ctcagagaaa gttttctaat gtcattctgt ccacatgcac
     1021 agaaactcgg cagagccgag attgaccggg cagaaggtcg gattttacct tccgtacaac
     1081 ggatgcacac atgaccgtgg acccgtttgg gaatatattt gcagcggact ataaatgaca
     1141 agagacgctc gatgtttacc ctcgcgcgag tggtttgttg gtaaccgttg attctggcga
     1201 tgaatccagc gcaaacagtt tatccactgc ttgccgattt ggttgtcttg gtccatctag
     1261 gctttgtcgt cttcgtgggt ttagggggct tgttgatgat gaagtggccc gggctcatct
     1321 ggatccattt gccggcggtg ttctggggca tcgtgatcga actttccggc tggatttgtc
     1381 ctttgacgcc gctcgagaac tggctccggc gcaaaggagg agaagaaaac tatcagtttg
     1441 attttgtcgc gcattacctc ttgccgatgc tttatccgca agggctaacg cgtagatcgc
     1501 agattctcct gggcgttctc gtgctgctcg tgaatgtcgc gatctatggc tgggtccgtg
     1561 gaaaacagaa aggttcaaca gttcaatagt tcaaacgttc aacgacctac taagattcaa
     1621 ctccgaagtt aatcgtctgc gaacggatca acccgagtaa gtaatgaaag ctccaactcg
     1681 ttgagttgaa ctcgggagca ctggcttgaa cttttgaacc aactgttaaa cgcaactcgt
     1741 tcttaatccg tttttgaaac gggcgcgagc ttgccactgc tgtcgtaaat ctcgataggc
     1801 ccgtattttt ccaaagcagg tttgatctta tccgcatcgc ccaccgccac cagctgcatc
     1861 gtccccggat cgagataggt acgcgccacg cgctgcagat cttccgccgt tactgccata
     1921 atttggcccg ggtagttgtc ccagtaatct ggaggaaagc cataggtttt ctgcaccatg
     1981 gcaaaagcca gcacgcccgt cggctgctcc agtgatagcg caaaccgcgc tgtgatagcg
     2041 cgtttggcat cagccaattc actatcggat actttttcat cacgaatgcg acggatctcg
     2101 ttgaagaact cagccaccgc gggagccgtc acttccgtgc gcatactgcc gccggcgcgc
     2161 cagggtccgg ggtagcgaag cgcgctcaga tcgctgtaga ctccgtaggt gtaccctttt
     2221 tcctcgcgaa gattcaaaaa caatcgggcg gcggcaccgc cgcccagaat gtgattcatg
     2281 accaccagcg gcacatagtc cggactgcgc cggtcgatgg cgatgttgcc cagcgcgacg
     2341 gtcgtttgca ccgagttggg ccgatcaacc aggtagactt tccgctcggt ggttggaaca
     2401 gcattggccg gcaaggtttc tttcaaactg gtcgcttccc aatcggccag ccatttctca
     2461 agtttgggga tcagttcgcc agcgcgcaca ttgccggcaa atcccagaat cgcattctgc
     2521 ggcacatagc gctcgcgatg ccacttcgcc aagaccgccg gtgtcaaaga ttcgagcgat
     2581 tctgccgtcg ctgtaaccac tgctgccgga tgattgccaa agagcgcgcg gttgaaacgc
     2641 tcgctggcga gaaaaccggg cgccgctctc tgctggcgca attgcacgga cagccgttgc
     2701 ttgaggttat ctaattcgcg tgccgggaaa ctaggatgga gcaagacctc caacgccaga
     2761 ggaaaccagg tgtcaaagtt atcgcttagt ccggaagcgc taaaagttgt gactgatgag
     2821 ccgaactccg ccgatccgcc gaaacttgca cccaatttgt cggcctcttc ggctatctgt
     2881 ttgctgtcac gcgacttcgt cccttcacgt aacatctgcg ccgtgacgct cgccagcccg
     2941 gcaacattct cgggttcgta caaagctccc gcggcgctga tctgcagttg gaccgaaacc
     3001 gccggaaagc gatgatcttc ggcgatcagc accgtaaggc catttttcaa tttcgcttct
     3061 ataggtttgg gcagcttgac tcgaaggacc tcgcttgaca ccggcgcttc gttttttctt
     3121 tctactttgc tggtcggcgt gctcggcgcc gtatcttgag cgccaagcgg cgccgtcaga
     3181 aagagaaacc caaccgcaag gctgattgcc gccgttaaag acccataaga tttaagggag
     3241 tgattcatat cagcgatgga tcgtcgcggt gggtgcggct gctttggctt tcggcaacgt
     3301 cgtcacgacc gttcgattgc tctccttcaa atagatattg gcaacacgct gcaggtcgtc
     3361 ctttgaaacc ttgaagatct tctcctgaac tgaattgatc agccctggat cgttgtaata
     3421 aacggcataa tgacccaggc tattggcacg accgcgggtg ctatagaaac tctgcgagcg
     3481 ctggcgacgg acctgggtgc gcaccttttc tatttcccaa tccatgacag cttcggtctt
     3541 ggctcgctcg agctcttgat aaacgagctt ctcaatttca tcgaggttaa ttcccggcct
     3601 gggcattact gaaacccaaa acagagatgg cccgcgacgt tcgtccggcc cggcggaaat
     3661 acttagggca agctcttggt ccttgacgaa tttctggtaa agccgcgatg ataatccgcc
     3721 ggataggacc tcccccaaca cctgcaatgc gtaggaatcc ggagtgttcc ccggcggaac
     3781 cttgtaaaca atgtcgagcc ggggcgtttg cgcaaaggga tcctctacgg ttttgcggcg
     3841 ttcagcctgt tgctccggtt ctgtggcatc gggaacaggc ggcggcgctt gtgatggaat
     3901 gttctcgaaa tattttttga tcagggccat agcgtcttcg gtcttgaaag ccccgaccaa
     3961 tgagagcacc gcattgttgg gcgcataata ggtccgaaag aactccgcca cgtctttgag
     4021 agtcgcagag tttaagtcct ccatggatcc gatggtggag tgcttgtagg cgaaattgtc
     4081 ataggcggtg ccgatgactg cttcataggt tcgaccatag ggccggttgt cgactccctg
     4141 gcgccgctct tcctggaccg tatggcgctg gttgtccaga ttggcctgat taatattcaa
     4201 agagcgcatc cgatcggctt ccaaaaatag gccgagctca agctggtttg ccggcaaagt
     4261 ctggaagtag ttggtccgat ccgcattggt ggtgccgttg gcgctgccgc cgttgtttaa
     4321 aaccaaaata aagtgctcgc ccttaccgac gtttgccgag ccctgaaaca tcatatgttc
     4381 aaagaggtga gcaaatcccg tgcgacccgg cttctcgtca cgagagccga cgttataggt
     4441 gacgcagatc gaataggttg gcgcatgctg atcttccgag agaataatac gcaggccgtt
     4501 ggcgagctta tattctttga aggggacagc gatggtatcg gcaggctgag cggcggcgtg
     4561 cgccggcgtt aattgtgatt gttcaatcgc ccccgcagcg acaagcgaca ggatgagcag
     4621 cgcaagcgga taagaaatgg ctttagacaa gtcaggctcc aaaattgaaa atttagacta
     4681 gccaccgcca gcccctcaca ggcgatgcaa aacagtcagt ccactcagta tattgggact
     4741 acgcgtgagc gcaagcgcgc tgctcgggtt cgctgcatcg gccggctact tttccatcaa
     4801 gatcatccgt taagcaatct cgcgatcatt cgaatccggt tgacagctct tatttctcag
     4861 cgataacgtc tgccatcaag acgatgaggt gagtttatat ggccagcccg gttaaggaat
     4921 ttgaagtcca tccttatgtc cagttcatga tggacaggta tagtccctat agaaagtgga
     4981 tcgaatccga aggcgtgccg atcatcggcg gtgcgttcgt caaggacgtg cgcaccgaga
     5041 aacttgaata ttggaagcgt aaagagtgca aaggggctat ttgcgcgttt tccgatcaga
     5101 tggttgctga cgcctacatc gctgaagtcg atccggggaa gagcaccaaa cctcaacgcc
     5161 agctctacga ggaaatcatc accgtcgctg ccggccgcgg ggcaaccacc gtttggtacg
     5221 aaggtacggc caaacgagtt ttcgaatggg aaagaggcag caccttcgcg attccactca
     5281 acgcttggca ccagcatcat aatgccagcg gctccgagcc ctgccgctat tttgcgctca
     5341 ccagccagcc ggtggcgttc gagcttttcc gggaccccga gttcattttc aacacaagct
     5401 atatgttcaa agatcgtttc gatcccggcg atcccgaatt tttctccaaa gaaggccagt
     5461 acttcaccga atattacggc ggcattctcc attccaactt catccccgat atccggaaaa
     5521 tcaatctcgt gccgcgcgag aagcgcggca aaggcacgcg caacatgtat attcacatgt
     5581 ccggcagcgc gatgttggct cacgtctcgc agttccctgt agggcggtac aagaaggcgc
     5641 atcgccatgg tcctggtgct catgtgtttc tgctcgactc caccgggtac actttgatgt
     5701 ggaatgaggg tgaaaaacca caaaggtacg actggcacga agcgacctgc atctcaccgc
     5761 ctgccgggac ttggcatcaa cactacaata ccggccagga gccctgtaag tttgttgcgc
     5821 tgcatgccaa cacggcggtg caaagagaag agaggggcgt cgagcaaatc gaattcgagg
     5881 acgaagatga ctcgatgcga aagctctacg aggaagaatg cgccaagaat ggagtcagaa
     5941 tggatatgta ggagtcgttt gtgagaagaa gcggcttctt aaaatctgcg cctcgagcat
     6001 tggaggtttc atgaatcaac tgagactcgc gtcagtgttc ttgatcgctt acctggtact
     6061 cgcgctggtt aatgtcttcg gcgcgagcga agcccgcgcg gcatccgctt cgaatccaac
     6121 ccgcgaaagt gaatcccaaa atgaatccat cgacgagctt tatgaaaaag ccaagaaaga
     6181 aggcggccag ctgacgcttt acatagccct gtcggcccgg tcggaagaaa tcatcctgcc
     6241 gttgttcaag aaacgctttc ccgccattgc agtgaatcac atcgacgcaa cctccgataa
     6301 gctggtcgcg cgcatattag cggagcagcg cggcggaaga attatcggcg acgtattcgg
     6361 cggcactccg ggttatttgg cgcaaatgac cgagcagaag ctgctggcgc cgcttgcgtt
     6421 acccgaagcg gcggcctatc cggcaatgct caaaggagca gagtgggtgg caaccgacac
     6481 gcaatatttt atcgccggat ggaacaccag tctggtccag aaagtcgatg agccgaagca
     6541 acttgaagac ttcgccgatc caaagtggaa aggcaaattg atcgccgagc cgcgggattt
     6601 tcagctgttg atgggtttgg ccaaaagaaa atacaaaagt gacgagaagg cggccgagct
     6661 attaaagaga atcgccggga atggagtgga gttccacaaa ggccattcgc agctgatcga
     6721 actgttggtg gcgggccagg cgccggtttg tctcacctgt tattcgcacc attttccgcc
     6781 gcgaatgaaa aaaggcgcgc cgattctgcc gctgttgagc gaaggcgtcg gcgaggtcgg
     6841 cggcagcgtg gcgattctca aaggcgcgcc ccacccgctt gcggctcggc tgtgggcgcg
     6901 ttgggccgtc agtgatgaag gacagagagc ctacgcccag gccggtgaaa ctccggctca
     6961 tccgaacgtc gagccggcgg agaaggtgag gcccgcgagc attcacatgt taactgccga
     7021 cgaggtcaaa gagtttccga agtacgagaa gctctggaag ggaatttttc agatccgata
     7081 gctagaacgg caagaccgtt ccaatgcgtt tcgccgccgc gcaatgatag atgtggttcg
     7141 ctagcgcgac atcctgattg accaggccga tggcgcggat gaagatgcgc tccttctcgg
     7201 attggcgcgt tggctttttt cccgatagca gctccggcag ctcggcgtaa aggtcctttt
     7261 cgctgataaa ctcttctttc agcaggcgct gcatgtcgga tttgttcttg cagtgtgccc
     7321 agctgtcgac gacgaacttg tcggctcgct tgtagaactc gctctccatc tcctggttct
     7381 tgccgatcga gtaaacggtg atcccctcgc cgagccagtt gtctttgaca aacggtgttg
     7441 aagttgttgt cgcggagact accaggtccg cgccgctcaa cgcttcttcg gtcgattcga
     7501 cgggtcgaat gttgagacca aattcggcgc cgatctctct ggcatagttc tggcgcgact
     7561 ggggcgtgcg cgaagtcaca cggatttctt tgagctgaaa tgcttcggcc attaccggca
     7621 cggcggcgcg ggcggtgtcg ccggcgccca gcatcgcgag gatcgcggcg ttcttgcgcg
     7681 cgagcaagcg cattgcgacg gtcgccgccg ctgcagtccg cactgcatgg caccaatcct
     7741 cgtccatgat ggcacggatc tctccgcctt cccggtcgct gagaatcaga attcttctcg
     7801 gtccccttgg cgcgcgcgac acatcgcggc taccacccgc ggctttaatc gagcgaatac
     7861 gaaatcctgc gaccggctca gttgaaagcg cgcagccggt gacccatgac tgccagcctt
     7921 gctccggctt gatcgcgaga tcttccggcg tcgaccagat cactcgacct tcgctgtgat
     7981 cgcgcagcgc ctgctcagta atttcgatag cgcggccgag gtcgagaact cttttgacgt
     8041 cctcgttcga taaatagaga atgccttttt gttccatgga ttttcaacaa cacatcgcca
     8101 gtaggaatcc gaataccgga tccgcaacgg acgcaaagaa tgcaatccta accacgggtc
     8161 ccaaccggcc tgtttggagt ttccgatttt cgaagaatcg atgttgttgc gattttgagt
     8221 ttcggatttt tcactcagca atttccgcct gttggttact tgataacgtc taacttgcga
     8281 cagccggtgg tcaaaaatac aggacctttc tcggtaatga gcacgtcttc ttcgagatga
     8341 accgcgcttt tttcggagcg aaccttcggt tccaaggcga ggaccattcc gggacgatac
     8401 agctcggtct cgacttcgtt aagcgatggc ggttcggctc tcgacatgcc gatgccatga
     8461 ccgatacgtt ttgggctctc cactgcctga taaccgcgct tgaccaattc acgattagcc
     8521 acgcgagaga cctcggcgat gggcgtgccg ggctgcatcg actcgaaaca aaggtcaatc
     8581 acgtcgcagg cggtctcatg atcttgtttg tgttcggcgc tcgcttcgcc aaagatcgtc
     8641 attcgtgtga cgtcgccgta gtagccttca taacaggcgc cgaaatcggc gatcatgcgg
     8701 tcgccctgat tgtagcggcg gtcgtcgtaa cgggtcgagg aattgagcat gagaaaacca
     8761 ggatgtcgcg ggtgggcgcc ttcctcgatc atcgatatgt agagccgatc cgcgacttcg
     8821 cgccgggtca tacctggtct caattgaggc aagcagcgat cgtacgcttt gacggaaata
     8881 gcacaggccg tacgcatgaa gtcgatctcc ttagggctct tgatcagccg catttcggtt
     8941 agcgcagcag tgccgtcttt gatttgtgct tttggtaaag cttcggttaa tcgtgaaaga
     9001 tagtttaccg gaaatccaag acgctgatcg tccccgagct caaaccccaa cttagattca
     9061 ccgagaccta actctttgag acaatcgccg atcatttctt caggaaaggg cacgtccgta
     9121 taggcgcgca cttcaccgat ccatggcagc gctttggctt tagctttgtc attgccatag
     9181 acgagcagca gcggcttgcc ggttttcggt aaaacgcaga tctgcggtcg ctggatatga
     9241 tcgagctgat aactgatgag accgctgaag taccagtaat tgtttttttc agagacgacc
     9301 aatgcgtcaa gaccgtcgcg ctccatcagc gtttgcgcgc gtcgatagcg ggtcaaatat
     9361 tcttcgtccg cgaacggggc ttcggttgcc atacctgctg cctccttgac cttcagcgaa
     9421 gacgcttgct aactcgcgaa agcttttctg ccttctactc gtttgagctt gcccacataa
     9481 tccagtgcct ccgtgagttg gatcacggtt gcgtacttgg cgttcatctc gaacaggttg
     9541 accaggtgcg aggtgcgggc gcgatcgaag acacactcct cgaccacaaa gaccggatag
     9601 ccgtaggagt aaccgtcgag cacggtgccg cgcacacagc cgcaggtgct ggttccggtg
     9661 acgagcaggc aatcgatgtt cttgcgcacc aggtaagtga tcagatgggt gccgaagaag
     9721 gcgctggcgc gggctttgtg gaccacgaac tcgccttttt ccggtttgat catttcggga
     9781 aatgccacgg ccagcttttc gaaatcagtg gcatcgacac cgcgtttggt ggcgttgccg
     9841 aacgcggctt tgaaatcagg atcgctggtc gaatatatca ccggcacgtc ggcatcgcga
     9901 caggcatgaa gcagctgttg aatattcggc aatgcatccc aagcgacttg gccgcagctg
     9961 gtgggaaact cgtcgatggc atccaggacg tgcataggtt tggtgccggt aaaaccggta
    10021 atgacgtcga tcaccaatag cgccgggtta cggccgaaac tgtccttgcc tttatagccc
    10081 gccttctcgt aaatctttct ttcttcttcc ggaataatct gttcccattc gcgcatgatc
    10141 gcctcctttt aactaagtct aggaggttgc tgaaaaaaag actcatagac ttcgttgcgc
    10201 tcgcttgact cgctgaggcg tacgcctcag ctcatcgaac gtcgcgcgcc ttggctctga
    10261 gatctttttg agcagcctct aatcagaatc ttttatcaga ctgctagcgg acccccaatg
    10321 cttcattgtg caatcgggtg atctcgcgca gattcttata attctccggc gtcaccacgc
    10381 ggcggcgaaa gccttcgaaa agtttcggcg gatcgggctt gacgtctttg cgcgggccga
    10441 tccggcgaaa tgttttaaga aactcctgcc cgtctttcga aaccagatag tcgataaaaa
    10501 gcttggacga gttcggatga gccgccttgg ccgacatcat cgcgatgttc agctccaccg
    10561 cgaccggctc caacggaatc caatccaccg gcgcgccgct ttgcttcatg cgctcgatta
    10621 cttgggcata gacgaaagtc atggagcgct caccgatgct gacgaaattg gcgcgattgg
    10681 tgttgccgcg caaaaagacc ggctcctgct tcgccaggcc tctgaagtaa ttcagtgcct
    10741 tttcctggcc ccattcctgg ctcaaggcga caaaaacgtc gtgattctcg atgtcgatga
    10801 tgaatttttg cttgtattcc ggcttgagca gatcgtcgta ggttttcggc agatcattgc
    10861 gcgaaatggc tttggtgttg tagccgagga ccagcgtatt cagataaatc gtcgtccaga
    10921 aacccttggg atcatagagg tcatcgtaga gattcgcagc ctccgacgtt acatacgggg
    10981 caatcaaatt gcgctcgcgc atcggcgtga gtgaaggcga aatacccagg gccacgtcga
    11041 acttgcttgc tcctgcctgg gcttccgtca tgatacggct taccatcgca gtgccgccga
    11101 ggcggaccag gtcgccgcga atgaacggat actttttggt aaacgcgtca agcaacggtt
    11161 tactgttctg gatgtccatg gtcgtataaa acaccagagc cccttccttt ttcgctcctt
    11221 caatgacgga cggttccgtg gcaagcgcaa aagggaccga gcacagccat actactaaga
    11281 tccacagtgg tcgagtgaac atggtgcttc cttgcatcga gctttcaacg ctgtttagca
    11341 tttggttttc ggatcagggg gaataggttt tatcgccccg ccgggtcatg aataagtcca
    11401 acggcaattc ggagccgata cctttttcac gagctttttc atacatcttt ctagcggtag
    11461 ctgcgaattg aatgcccata ccggtattgt tcttgaacag cgtgatctct ttgtcgtcga
    11521 tacgcctgcg gcacttgccg tttagcagat cgccgagctc gtgcagatcc tccgctttga
    11581 cgaggccgcg ctccaagcgc tcgtgaaact ctgcctgccg atcgataaaa atttgcggct
    11641 tgtaaccgac gacaatcagg gcggccctgg taatcacgtc atcatccagc tctttgcgcg
    11701 gcagaaagcc gtcgccgcca accaggctgg tgatgtgcat gccatcttcc agccagcggc
    11761 cctgaaccac gggatccacg gtgttggttg cagcgctgac gatatcgctg ccgcgcaccg
    11821 cttcttcggc actcgttacc ggcaacacct cgatgcccgt gctcgcgctc atctctcggg
    11881 cgaac
//
LOCUS       DASUVS010000450        15282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_199962, whole genome shotgun sequence.
ACCESSION   DASUVS010000450 DASUVS010000000
VERSION     DASUVS010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 15282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15282
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199962"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..886
                     /locus_tag="VF182_23695"
     CDS             14..886
                     /locus_tag="VF182_23695"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX6440141.1"
                     /translation="MIEEAFPGLRLFNRGKVRDIYDLGENLLLVATDRISAFDVILPT
                     LIPGKGKILTALSEFWFGKIKDITPNHLITTCFDEFPAACLPYRDKLDGRSMLVKKSI
                     PAPVECIVRGYLAGSGWKDYQNTGSVCGIPLPQGLVEASRLEHPIFTPSTKAAIGAHD
                     VNISFDEMLTKLGKKRAEAMRDSSIAIYQRASELAEKKGIIIADTKFEFGLETDQVIV
                     IDEVLTPDSSRFWPADGYRPGKTPDSFDKQFVRDYLLNLPWDMKSRPPELPPEIVRKT
                     QDKYAEALRRLTAV"
     gene            complement(1159..1689)
                     /locus_tag="VF182_23700"
     CDS             complement(1159..1689)
                     /locus_tag="VF182_23700"
                     /inference="COORDINATES: protein motif:HMM:NF013624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440142.1"
                     /translation="MRMLVFGFGPYGEFRNNITEAIIKALPPLPGLRTAVFPVRFNRR
                     QFIDVLEQHSPDCVIGLGQCTRDRIELETRARNRKRPGNRGLAKPIRKSGTEFLKTNL
                     DIKLGSDVGRSDNAGDYVCNYSMYVMLEYISRSGRDIRFGFLHIPDNFDSGTGVALLM
                     QAIAQIQVDSKLASRK"
     gene            complement(1797..3752)
                     /gene="thiC"
                     /locus_tag="VF182_23705"
     CDS             complement(1797..3752)
                     /gene="thiC"
                     /locus_tag="VF182_23705"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX6440143.1"
                     /translation="MSEGNKQIGSNQNGITTQPLPASHKLYVQSPKFSDVRVAMRAVH
                     LSSSGNGHLSNGNGDYKNPPVIIYDTSGPYTDVNAETEIRTGLPPLRLDWIKSRDVEE
                     LASSSYRPLGGKNGIQGVQTERFPDASRRPILRAKPGCNVSQMHYARQGIVTPEMEYI
                     AIRENLGREYAVANGANGTGRIHRGQSFGASIPDFVTPEFVRDEVARGRAIIPANINH
                     PEAEPMIIGRNFLVKINANIGNSAVASSIEEEVEKMIWATRWGADTVMDLSTGADIHE
                     TREWILRNSPVPIGTVPIYQALEKVGGKAEELTWEIFRDTLIEQAEQGVSYFTIHAGV
                     LLRYVPLTAKRVTGIVSRGGSILAKWCLAHHKENFLYTRFEEICEIMKAYDVSFSLGD
                     GLRPGSIADANDEAQFGELETLGELTKIAWKHDVQVMIEGPGHVPMHMIHENMTKQLE
                     VCHEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWYGTAMLCYVTPKEHLGLPDKKDV
                     KDGVITYKIAAHAADLAKGHPGAQIRDNALSQARFEFRWEDQFNLSLDPETAREFHDE
                     TLPAQGAKVAHFCSMCGPHFCSMKITQDVREYAAQKGLEDQAALDAGMQEKAEEFKRT
                     GGKLYHEMAGDGARNTEAGYTATAAKE"
     gene            complement(3806..4375)
                     /locus_tag="VF182_23710"
     CDS             complement(3806..4375)
                     /locus_tag="VF182_23710"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEX6440144.1"
                     /translation="MKRIEEMNLWERLYFPEVIRGLTITGYRFWRNLIVHALQQLGLA
                     KDVQASVTVQYPEARSPYPDTFRGRHRLTLKDDNSVQCTACFLCATACPAECIYIEAG
                     EYPDKPVEKFPIRYEIDTLRCIYCGFCVEACPCDAIRMDSGTHPGNLGLSRKDFVEDK
                     ETLMQRSRDLAEKGQEGLYEEYVKPYRHV"
     gene            complement(4528..5178)
                     /locus_tag="VF182_23715"
     CDS             complement(4528..5178)
                     /locus_tag="VF182_23715"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX6440145.1"
                     /translation="MGLISAADKIYVEDGYLTTFLKFGLLDGCAQNVSSSGMNNNEIE
                     SIRQAIRDVRDFPKPGILFKDITPLLSSGPLFAKTIDIFADRYRDQRIDTVLGIESRG
                     FIIGAALAYTLNAGFSVVRKPGKLPYNTHSASYDLEFGTDSLEIHIDAMPAKARVVIA
                     DDLIATGGTAAATAKLVSKLGAKVVECAFVIELSFLKGRDKLKPYDVFSILRYDDE"
     gene            complement(5169..6317)
                     /locus_tag="VF182_23720"
     CDS             complement(5169..6317)
                     /locus_tag="VF182_23720"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX6440146.1"
                     /translation="MKELASMRNDVVGSLLRPARLKEARVSYDEGRLSAAELRAMEDQ
                     CIRDAVRLQEEAGLAIVTDGEYRRLNFQDSFGESVSGFDAGRPSLKFYESRVEGSSPL
                     QRWEIPEQGEHKSTAVAQRRPVVEKLRLSRNAPLDEYRFVSQVAQRPAKVTLIGPDRI
                     SQRFDWQNSSAVYTGMDEFVADVVRVEREMIGAVVAAGCRYIQIDAPGYTAYVDKPSL
                     DAMRARGENPDENFSRSLRADNAVLDGFDNVTFGIHLCRGNQRSMWHREGTYDEIAER
                     LLNELKHDRFLFEYDTPRAGGFEPLRFLPKGKIIVLGLVSTKVPQLETVEQLKRRIDQ
                     ASRYAPLEQIAISPQCGFSSDVVGNLVSEDDQKRKLAVVAETSRQVWG"
     gene            complement(6323..7087)
                     /locus_tag="VF182_23725"
     CDS             complement(6323..7087)
                     /locus_tag="VF182_23725"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6440147.1"
                     /translation="MARLSAGSPYRLLGLMLFWHIAGCATQTPVQPPARGGVYHVVKP
                     GETLYRIGKAYDLNYAELARLNRLLDPNQIRVGQKLFIPGAARQLPVEVITPADSIVT
                     IPSVSRSSDLADDAIVWPILGNINSHFGRRGASFHDGIDIAAQEGSPIRAIGKGEVIY
                     SDQLRGYGNLVILRHSGGLVSVYAHNQVNLVAAGEFVAQGDIIGKVGSTGRVTGPHLH
                     LEIRKNNIAQDPLLYLPQLCCRQSSDSVDAEKSITE"
     gene            complement(7075..7689)
                     /locus_tag="VF182_23730"
     CDS             complement(7075..7689)
                     /locus_tag="VF182_23730"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX6440148.1"
                     /translation="MVQDQLISRGIQDRRVIAAMRRIPRHLFVDPGLVNRAYDDSALP
                     IGEKQTLSQPYMAGKMTEALGLSGKEKVLEVGTGSGYQTALLAELCFNVFSVEKIRAL
                     SRKARALLDRLAFQNIALHVGDGTVGWSEHAPYDAIIVAASAPDLPRPLIEQLSVRGK
                     LIIPVGDDESQVLKLVHREPFGFHQEDLGDCRFVKLRGKFGWQD"
     gene            complement(8046..8966)
                     /locus_tag="VF182_23735"
     CDS             complement(8046..8966)
                     /locus_tag="VF182_23735"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX6440149.1"
                     /translation="MSFDSIIDDFTAVILAGGKSSRMGRPKALLPFGNEPLVAQLVRR
                     LEGKFKMVVIVAAPDQELPPLPAKVVRDEVAYQGPVGGIYYGLLAANGTGAFVTSCDA
                     AFLSLPLISFLTSQLSDYDVVVPYWQDRFQPLHAVYRRSVVPLLKEQLERRELRPIFL
                     YDRVRTRKVTEQEIRRYDPEGLSFFNMNAPDDYAQALRLWQQRESFTNLTGEASLSCT
                     VELFGVARLLAKTRIISLSLAPDATFAQVLSAVAQRLPVLVGRVINPNGTDLLPGFAC
                     NINGIDFVRDRETKIRQHDKIFILSADAGG"
     gene            complement(9691..9767)
                     /locus_tag="VF182_23740"
     tRNA            complement(9691..9767)
                     /locus_tag="VF182_23740"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9731..9733),aa:Pro,seq:ggg)
     gene            complement(10040..10393)
                     /locus_tag="VF182_23745"
     CDS             complement(10040..10393)
                     /locus_tag="VF182_23745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6440150.1"
                     /translation="MTTSLPDKIYFKIGEVSEIVGVEPYVLRYWETEFDLLKPSKAPS
                     RHRLYKKKDVELLLDIKRLLYTEGFTIEGARKKLKEVKKEEKNQLKLPLSDQKYKTAL
                     AKIKKDLESLRRLLS"
     gene            complement(10394..10708)
                     /locus_tag="VF182_23750"
     CDS             complement(10394..10708)
                     /locus_tag="VF182_23750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEX6440151.1"
                     /translation="MTKAEIITRIYEKVGFSKKDATDVVEATFDIIKGCLEQGQKVKI
                     SGFGNFVVNTKRPRKGRNPQTGDEIIIVGRKVLTFKASQILKKSLNGMSSPTESSPAE
                     AE"
     gene            complement(10738..12825)
                     /gene="pheT"
                     /locus_tag="VF182_23755"
     CDS             complement(10738..12825)
                     /gene="pheT"
                     /locus_tag="VF182_23755"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX6440152.1"
                     /translation="MKFTFNWLKELVEIDASANELSELLTMAGLEVESLAPLTGSEAD
                     ADDWVFEISVTPNRGDCLSILGLAREVAALTDKPLKNRRSDVQAKVTPYLGNVGIKIV
                     NPELCSRYSAFVIDNLRISQSPKWIQHRLEACGFRSVNNVVDVTNYVMVEVGQPLHAF
                     DLDRLTSDHIVIRQASGEREFVALDGVKRELADGDLLICDGNTPVALAGIMGGQNSEV
                     TAATRRVLLESAHFDPTTIRRTAKRLGLHSEASHRFERGVDPAGTVSAGNRAAQLISS
                     QAGGSVSSGVLDSYPNPPQPPSILLRQDRIKRVLGVEIALVEAERILTALGLNAKKAA
                     GEDSLMVVPPTSRGDLTREADVIEELARLFGYQKIPTTLPVSRSTAGKADYQLMWERR
                     LRSFLAGEGLVEVINLPFTNETLNQMFTGLWNGPTSPVAVLNPLVKDSAEMRLSLLPG
                     LIENLRLNLAQKAEGFFAYHLGKVFRLHANGEIEEKQCLGGLLFGSRRRKGLRSSDGL
                     RPVRFLDCKGIIEGILDLFHLRESILWSRANVTVLHPGRSAQLEHDSEMIGYVGESHP
                     DLNDQFGLPPLLAFELDFEKLLQYSPRQIRASSLPRFPAVERDFAIVVDQAVSSQKII
                     SCINELGQSLIKGIEIFDEYRGDSIPHGKKSLAYRIYYRADDRTLTDSEVNTMHQQVL
                     DHIGKLLQAHRRS"
     gene            complement(12864..13904)
                     /gene="pheS"
                     /locus_tag="VF182_23760"
     CDS             complement(12864..13904)
                     /gene="pheS"
                     /locus_tag="VF182_23760"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX6440153.1"
                     /translation="MIDSLDSLRDQILARIEKSGSEAEIEQIRVEALGRKGRLTLLLR
                     GLKDLPLDQRPSAGERLNALRQLLESRLQERLQVVKRQQKEKALREESIDVTLPGNRW
                     ESGRIHPLTLVIDEIIDVFGGMGFEIARGPDIEDDYHNFEALNIPKDHPARDMQDTFF
                     VSDGRLLRTHTSPVQIRTMESRQPPLQVIVPGAVYRHDDDATHSPMFHQVEGFMVDRD
                     IAFSDLKGVLTHFLQRMFSEKAGVRFRPSFFPFTEPSAEIDIQCVICGGDGYAAQQQH
                     CRVCKGSGWLEILGAGMIDPEVFKFVGYDPERVSGFAFGMGVERIAMLKYGIDDIRLF
                     FQNDMRFLRQFG"
     gene            complement(13924..14283)
                     /gene="rplT"
                     /locus_tag="VF182_23765"
     CDS             complement(13924..14283)
                     /gene="rplT"
                     /locus_tag="VF182_23765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX6440154.1"
                     /translation="MPRAKGGSKTRQRRKKVMKMAKGYVGGRGRLYRSARETVERALA
                     FAYRDRRVKKRTFRGLWITRIGAAAKLNGLSYSQLIKGLKQARVEIDRKVLAEIAVAD
                     MDGFNQLAQRAKSSLDA"
     gene            complement(14326..14523)
                     /gene="rpmI"
                     /locus_tag="VF182_23770"
     CDS             complement(14326..14523)
                     /gene="rpmI"
                     /locus_tag="VF182_23770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX6440155.1"
                     /translation="MPKIKTNRAAAKRFRVTASGKIKRNKGFKRHLLSSKGKKRKRQL
                     RSGTMVSAAEQRNIRELIPYK"
     gene            complement(14555..15058)
                     /gene="infC"
                     /locus_tag="VF182_23775"
     CDS             complement(14555..15058)
                     /gene="infC"
                     /locus_tag="VF182_23775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX6440156.1"
                     /translation="MARDARINHQIRAREVRVIGAQGEQLGILPLHEALRQVDALGLD
                     LVEVAPEAQPPVCRMMDYGKFRYQQRKKTHGPKKHTVVEIKEVKMGSRTDAHDVEHKV
                     RNIRQFIEKGQRVKVSVFFRGREITHPELGRQALRKVVEQIQDIAKVDIEPRLEGRNM
                     AMLLTPK"
     gene            complement(15094..>15282)
                     /locus_tag="VF182_23780"
     CDS             complement(15094..>15282)
                     /locus_tag="VF182_23780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX6440157.1"
                     /translation="LGYKIREAQLAKIPYAIVIGDSEVQAQTVAPRRRGGENLAPMSL
                     NEFMERLRSEVAQERGEA"
BASE COUNT         3527 a         4267 c         3820 g         3668 t
ORIGIN      
        1 tgagcctatt tcgatgatcg aagaagcctt tcctggtttg cggctcttca atagaggcaa
       61 agtacgggac atctatgacc taggtgagaa tctcttatta gtcgccaccg atcgcatctc
      121 tgcgttcgac gtcatattgc cgacgctcat tcccggcaaa ggcaagattc tgaccgccct
      181 ttcagaattc tggttcggca aaataaaaga catcactcct aatcatctca ttaccacttg
      241 tttcgatgag ttccccgccg cgtgcctgcc ctatcgcgat aaactggacg gccgatcgat
      301 gctggtcaag aagagcatac cggccccggt ggaatgtatt gtgcgcggtt acttggcagg
      361 atctggctgg aaagactacc agaataccgg gagcgtgtgc ggtatcccgc ttcctcaagg
      421 tttagtggaa gcgtcgcgcc tggagcaccc gatatttacg ccgtccacga aagctgcgat
      481 cggcgcgcat gatgtcaaca tcagcttcga cgagatgctg acgaaactcg gcaagaaaag
      541 agcggaggcg atgcgcgatt cgagcatcgc gatttatcag cgggcgagcg agttggccga
      601 gaaaaaagga atcattatcg ccgataccaa attcgaattc ggcctcgaaa ccgatcaggt
      661 catcgtaatc gacgaagtcc ttacgccgga ttcatctcgc ttttggccgg cggatggata
      721 ccgccccggc aaaactccgg atagttttga taaacaattt gtgcgcgatt acttgctcaa
      781 tctaccatgg gacatgaagt cgcggccccc ggaattaccg cctgaaatcg tcaggaaaac
      841 tcaggataag tatgccgagg ctttgagacg gcttactgca gtttgacccc aaacgaccgc
      901 atttcgaacc gctagggcac gattatcctc ctcgaatcct ttggctaccc acgatccgcc
      961 agcttgcgcg tgctgcaata gtcttttttg cacctagtct cgagttgtga tcttcttcgg
     1021 aagaatatcc gctgctcaag ttcaactctt tacgcggtgt ccggcagcgg tgcgcgattc
     1081 tcagggttgc gccgcgcttt tatcgcggag aggcgaaatc aatccgttat attgttcgcg
     1141 ccagcgagta gagcgaactt atttccggga tgcgagtttg gagtcgacct gaatttgggc
     1201 gatcgcctgc attagcagcg caacgcctgt tccggaatca aagttgtccg gaatatggag
     1261 aaacccaaac ctgatatctc gcccgctgcg gctaatgtat tcgagcatta cgtacatcga
     1321 atagttgcag acgtaatctc cggcgttatc ggaacgacca acatccgaac caagtttgat
     1381 gtcgagatta gtctttagaa attcggtgcc cgatttacga atcggtttgg ccaaccccct
     1441 gttgccgggc cgcttgcggt ttcttgctct ggtttcgagc tcaattcgat cacgcgtgca
     1501 ttgccccaag ccaatcacac aatcgggact gtgctgctcg agcacatcaa taaactgtcg
     1561 ccggttaaag cgcaccggaa agaccgccgt tctcagcccg ggaagaggcg gtagagcttt
     1621 aatgatcgcc tctgtaatgt tgttgcggaa ttctccataa ggtccgaagc caaagacgag
     1681 catacgcatc gcgcaatctt acaaggatgg tttcatagta gacccgtagc ttgaaagacc
     1741 aaaaaaaact ggccgacatt tgccggccag tttttcgcaa tcagttaata ttgcggctat
     1801 tccttggctg ccgtcgccgt gtagccggct tccgtgttcc tggcgccatc gccagccatc
     1861 tcatgataaa gtttgccgcc cgtccttttg aactcttcgg ctttttcctg catccctgca
     1921 tccaaagccg cctggtcttc caagcccttt tgtgccgcat actcgcgcac gtcctgagtg
     1981 atcttcatgg agcagaagtg aggaccgcac attgaacaaa aatgggccac tttcgcgcct
     2041 tgcgccggca aggtctcatc atgaaactca cgcgcagttt ctggatccag gcttaaatta
     2101 aactgatctt cccagcgaaa ttcaaagcgc gcctggctca atgcgttatc gcggatctgc
     2161 gcccccggat gtcctttggc caaatccgca gcatgagcgg cgattttgta agtaatgaca
     2221 ccgtctttaa cgtccttttt gtccggcagg cccagatgct ctttcggtgt gacatagcaa
     2281 agcatcgctg tgccgtacca cccgatcatc gccgcgccga taccgctggt aatatgatca
     2341 tagccggggg cgatatcggt cgtcaggggg cccaacgtat aaaaaggcgc ctcatggcaa
     2401 acctccagct gtttcgtcat gttttcgtgg atcatatgca tcggcacatg gccgggccct
     2461 tcgatcatca cttgcacgtc atgcttccag gcgattttcg tcaactctcc aagagtttcc
     2521 agttcgccga actgagcctc gtcattggcg tcggcaatag aacccgggcg tagcccgtcg
     2581 cctaaggaaa acgacacgtc atacgccttc atgatttcgc aaatctcttc gaaacgggta
     2641 tagaggaagt tctctttgtg atgcgccagg caccatttgg cgagaatcga tccgcctcgg
     2701 gaaacaatgc cggtcacccg ttttgccgtg agcggcacgt agcgcagcaa gacaccggca
     2761 tgaatggtga agtacgagac gccttgttcc gcctgctcga tcaatgtatc gcgaaagatt
     2821 tcccaggtga gttcctcggc tttgccgccg accttctcca aagcctgata aatcggcacg
     2881 gtgccaatcg gcaccggaga gttgcgtagg atccattccc gtgtctcgtg aatgtcggcg
     2941 ccggtggata aatccatcac cgtatcagcc ccccaacggg tagcccagat catcttttcg
     3001 acttcctctt cgattgaaga cgcaaccgca gagttgccga tattcgcatt gatcttaacc
     3061 agaaaattgc ggccaatgat catcggttcg gcctcgggat gattgatatt ggccggaatg
     3121 atggcgcgcc cgcgcgccac ctcatcgcga acaaattccg gcgtgacgaa gtcgggaatc
     3181 gatgcgccga aactttgacc gcggtggatg cgtccagttc cgttagcacc gttcgccacg
     3241 gcgtactcgc ggccgagatt ttcgcgaatg gcaatgtact ccatttccgg ggtgactatc
     3301 ccttgccgtg cataatgcat ctgcgacacg ttacaaccgg gtttagcgcg caaaatcggc
     3361 cgccgcgaag catcgggaaa tcgttctgtt tggacgccct ggataccgtt cttgccgcct
     3421 aacggacggt agctactgga cgccagttct tcgacatcgc gacttttgat ccagtccaat
     3481 cttaacggcg gaagacctgt tctaatctca gtctcggcgt tgacatcggt ataaggaccc
     3541 gaggtatcgt agatgataac aggcgggttc ttgtaatccc cgtttccatt gctcagatgg
     3601 ccgttgccgc tgctcgataa atgaaccgcg cgcatggcaa ctcgcacgtc cgaaaacttt
     3661 ggactctgga cataaagctt gtgcgatgcc ggcaatggct gagtggtgat tccattttgg
     3721 ttcgagccga tctgcttgtt tccctctgac atacgatacc tcctcggaac ctgcgctgcg
     3781 ctattagggt tgatggaatt ggttcttaga catggcggta tggctttacg tattcttcat
     3841 acaggccttc ctggcccttt tccgccaaat ctcgggaacg ctgcatcaag gtttctttgt
     3901 cttcgacaaa atccttccgg ctaagcccca agttgcccgg atgagtgccc gaatccatcc
     3961 tgattgcatc gcacggacag gcttcgacac agaagccgca ataaatgcac ctgagggtgt
     4021 cgatctcata gcgaatcgga aatttttcca ccggcttgtc aggatactct cctgcttcga
     4081 tgtagatgca ctcagcagga cacgcggtgg cgcagagaaa acaagccgtg cactgcaccg
     4141 agttgtcgtc tttcaaagta agacggtggc ggccccgaaa cgtatcaggg taaggcgatc
     4201 gcgcttcggg atattgcaca gtaacggacg cttgaacatc tttggctaac ccgagttgtt
     4261 gcagggcatg aacgatcaag ttacgccaga accgataacc ggttatcgtc aaacctcgaa
     4321 tcacttcggg gaagtacaag cgctcccaga gattcatttc ttcgatacgt ttcatcagta
     4381 ttcctagcta gagtgcaggt gaaccatgaa cccaagagat gcgcgaaggc agcatctcgg
     4441 ctccgctggc cgcgggtcga tttactctac cgatcttggc agacggttgt caaggaacga
     4501 aaccgcgcaa aaacaggcgc ttgggtgtta ttcgtcgtcg taccgcaaaa tcgaaaaaac
     4561 atcgtacggc ttgagcttgt ccctaccttt caaaaacgaa agttcgataa cgaatgcaca
     4621 ctccaccact ttggcgccaa gcttcgagac cagcttcgcg gtcgcagccg cggttccgcc
     4681 cgtggcgatc aaatcgtcgg caatcaccac gcgggccttg gccggcatcg cgtcgatgtg
     4741 gatctccagt gaatccgtgc caaattccaa gtcataactg gcgctgtgag tattgtaggg
     4801 caacttccca ggtttgcgaa cgacactaaa tccggcattt aatgtatagg caagcgccgc
     4861 accgattatg aacccgcgtg attcaatgcc taaaaccgta tcgattctct ggtcccgata
     4921 acgatctgcg aagatgtcga tcgtcttggc gaagagcggg ccactactta gcaatggcgt
     4981 aatgtccttg aataaaatcc ctggttttgg aaaatcgcgc acatcgcgaa tggcctgccg
     5041 aatactttcg atttcgttgt tgttcatgcc gcttgagctc acattctggg cacagccatc
     5101 aagaagcccg aattttaaaa atgttgtaag ataaccatct tcgacgtaga tcttatcggc
     5161 cgcagagatc agccccacac ctgccggctg gtttccgcca ccacggccaa ttttcgtttc
     5221 tggtcgtcct cgctgaccaa attgccgacc acgtcggatg aaaaaccaca ctgcgggcta
     5281 atggcaattt gctccagcgg cgcataacgg cttgcttggt cgatgcgcct tttcaactgt
     5341 tcaacggttt ctagttgcgg caccttggta ctgaccagtc ccaaaacgat gatcttgcct
     5401 ttgggtaaaa accgtagcgg ctcgaatcct ccggctctcg gcgtgtcata ttcgaacaga
     5461 aagcgatcgt gcttcaactc attgagaagc cgttcagcaa tttcatcata cgtgccttcc
     5521 cggtgccaca tgctgcgttg attgccgcgg caaagatgaa ttccgaaagt cacgttatcg
     5581 aaaccatcga gaaccgcatt gtctgccctc aatgagcggc taaagttctc atcgggattt
     5641 tcaccgcgcg cgcgcattgc atcgagcgac ggcttatcga cataggccgt atatccgggc
     5701 gcatcgatct gaatataccg gcaaccggca gccaccactg cgccgatcat ttctctctcg
     5761 accctcacga cgtcagccac gaactcatcc atgccagtat agaccgcgct agaattctgc
     5821 caatcgaagc gctgtgaaat gcgatcgggt ccaatgagcg tcaccttagc cggccgctgc
     5881 gccacctggc ttacgaaacg atattcatcc agcggagcgt tgcgcgagag ccgaagcttt
     5941 tccacaaccg ggcggcgctg ggcgacagcc gtagacttat gctcgccttg ctccggtatt
     6001 tcccagcgct gtaacggact acttccctcg acccggcttt cgtagaactt cagactcggc
     6061 cgacccgcgt cgaaaccgga tacggattcg ccgaagctat cttgaaagtt caagcgccgg
     6121 tattcaccgt cggtcacgat cgccagaccg gcttcttcct gcaatcgcac cgcatcgcgg
     6181 atgcattgat cttccatggc ccgcaactca gctgcgctta gcctaccctc gtcatagctt
     6241 acgcgcgctt ccttcaatct agcgggccgc aataaactac cgacaacatc gtttcgcata
     6301 ctggccagtt ccttcattta catcactccg taattgactt ttccgcgtcc acgctatcgg
     6361 atgactgacg gcagcacaac tgaggaaggt agaggagcgg atcctgcgcg atgttattct
     6421 tgcggatctc gagatgcagg tggggccccg tcactcttcc ggtgctacct actttcccga
     6481 tgatatcccc ttgagcgaca aactcccccg cggccacgag atttacctga ttatgcgcat
     6541 agaccgaaac caggccgccg gagtgccgta gaatcaccag gttgccataa ccgcgcagct
     6601 gatcactgta aatcacctcg cccttaccga tggcccggat cgggcttcct tcctgggcgg
     6661 caatatcgat accgtcatgg aaactggcgc cccgccggcc gaaatgggaa ttgatgtttc
     6721 ccaggatggg ccagacgatg gcatcgtccg ccagatcgct ggacctggag accgacggga
     6781 tggttacaat cgaatcagcc ggggtgatga cctctacggg caactggcgg gccgctcccg
     6841 gtatgaacaa tttttgtccg acacgaattt ggttcggatc taggagccga ttaagacgcg
     6901 ctagctccgc gtaattcaaa tcatatgctt tgccgatccg atataacgtc tcaccgggtt
     6961 taacaacgtg ataaacgcct cctctagcag gcggctgcac tggcgtctgg gttgcgcagc
     7021 cggcgatatg ccaaaacaac atcaagccga gtaaccggta aggagagccg gcgcttagtc
     7081 ttgccaaccg aattttcccc ggagtttaac aaaccggcag tcaccaagat cttcctgatg
     7141 aaagccgaac ggttcacgat gaaccagctt caacacctga gattcatcat cccccaccgg
     7201 tattatgagc ttaccccgca ccgatagttg ttcaatcaag ggccggggaa gatcgggagc
     7261 actggcagca acgataatgg catcgtacgg cgcatgctcg gaccagccta cggttccatc
     7321 tcctacgtgc agcgcgatgt tctgaaatgc gagccggtct agaagcgccc ttgccttgcg
     7381 cgaaagagcg cggatctttt ccaccgagaa aacgttgaaa cagagctcgg caagcagagc
     7441 agtttgatag cctgatccgg tgccgacttc taaaactttc tctttaccgc ttaggcccag
     7501 cgcttcggtc attttcccag ccatgtaggg ttgcgagagc gtctgcttct cgccgatggg
     7561 caacgcgctg tcatcatagg cgcggttgac taggccgggg tcgacaaaaa gatgtcttgg
     7621 aatgcgccgc atggccgcga taacacgccg gtcctgaatg ccacgagata taagttgatc
     7681 ttgaaccatt ctttctctgg cccaagcgta atccggaggg gtcgtatcgg taagcttcac
     7741 gcgtttttaa ctggtttcga tccccgcttg acgatctgag taaccgtcgt tagcgagcca
     7801 aggattttcc ttgcagatca caaaatgagt tgatgctcca agattcgttg cggagtgccg
     7861 gctggcggcc aaatccgcgg gtattttcta ccatagcaag aaacggtttt ctattagtcg
     7921 gtcgttggaa cgcatcgtcg cgtccagaga tggttgatac gcagctagcg gcggcgaata
     7981 atttttttcc attactcctc gattcttttt catgaaccaa ggtgtgctca tgatgtagat
     8041 cagtgctatc ctccagcatc cgcagagaga ataaaaatct tatcatgctg gcgaatcttg
     8101 gtctcccgat cgcgcacaaa atcgatgccg ttgatattgc aagcgaagcc gggaagtaaa
     8161 tccgtgccgt tcggattgat aacacggccg accaataccg gtaacctctg tgcaacagcc
     8221 gacaacacct gagcgaaagt agcgtctggc gctaaagaaa gagagattat tcgggtcttt
     8281 gcaagcaagc gggcaacgcc gaacagttca acggtgcaac taagggacgc ctctcccgtt
     8341 agattcgtaa aactctcgcg ctgctgccac agccttaaag cctgcgcata atcgtcaggc
     8401 gcgttcatgt tgaagaaact caagccttcg gggtcgtagc ggcgaatttc ctgctcggtg
     8461 actttacgcg tccgcactcg atcgtaaaga aatatcggcc ttagctcgcg ccgttcgagt
     8521 tgctctttca gcagcggtac tacgctcctt cggtaaactg cgtgaagagg ctgaaatcga
     8581 tcctgccagt atggaacgac cacgtcgtag tcggacagct gcgaggttaa aaaagaaatc
     8641 aatggaaggc ttaaaaatgc tgcatcgcac gaagttacaa aggcgcccgt tccgtttgcc
     8701 gccagcaaac cataataaat gccgcctacc gggccttggt aagcgacctc gtcccggacc
     8761 accttggcgg gcaacggcgg cagctcctgg tccggcgccg ccactataac gaccattttg
     8821 aatttgccct cgagccggcg cacgagttga gcaaccaagg gctcgttgcc aaacggcagc
     8881 aaagccttgg ggcggcccat acgtgagctt ttgccgccgg ccagaataac cgcggtaaaa
     8941 tcgtctatga tcgagtcaaa actcaactgc tactctccga tggcgattct tatcacgtct
     9001 tcagctctcg cgcagccgtt tggctcaatc cgagaaattt actcaagttt tgatacctca
     9061 ggaaggagat acaatcgtgc ttggctttcc ttaaataatt ggaacagccg tcgcatgctc
     9121 gcacaaagag atgaatcgct tcaggatgag gagtgttcga acgggaatcc gacagtcagt
     9181 tccaagtaac ctcgttgtga gtcaaggatt cgttcccgtc ttcatcgagg tccaggatcg
     9241 gatcgcacca acataattcc gaattaatat gagccaacgg gacagcatca gggacgtcgg
     9301 acggatatcg agaagccaga tgttgttttg agatcatggc agttcctccg gcacacctac
     9361 gtgcgcagca aatcatgtgc cggaaccaac ggccagaaca atgaatctag aggaaggatg
     9421 atcaaacagc taaaatgatg tctgctgacg tgtgctatag ctatggatgg agagattttg
     9481 atccaacaat ggttcatagc ttcagttaca atcatcgttt atgtcccaga acgcggaata
     9541 cataagcgac gcgcattcaa accggttaac cgcctcacga gaagctcacc tccgcggcgt
     9601 gagtcactcg gatacacaaa cgccaattca agacggagca actaacgtca atccggatgg
     9661 ttgtgctatg gcgatagaaa cagaacagaa tggtcggggc gaccggattt gaaccggtga
     9721 ccacacgcac cccaagcgtg tgcgctacca ggctgcgcta cgccccgaca agctgtttag
     9781 agtaggcgtg actctaacat ccgatattcg ccaaatcaat ttagtgcgcc gcggttagtt
     9841 aagttaatcg caagaaagcc tctagaaccg aacatcgcta attgcacgaa gacatcggcg
     9901 atatctaagt tgagcagggc gtctacgtct ctggggagat tgtctgaaaa gagatagttc
     9961 tgacggatgg ttgccaagct ggacaaccat tccaggacca gcctctgagc tacgtccgga
    10021 tcacggactt cctattgcat caagaaagta aacgtcgaag cgactcaaga tctttcttga
    10081 tcttggctag agcggttttg tacttctgat ccgagagcgg aagcttcagc tgattttttt
    10141 cctctttttt tacttctttc aatttcttgc gggcgccttc gatagtaaac ccttcggtat
    10201 agaggaggcg cttgatgtct agtagaagct cgacgtcttt cttcttgtag agacgatggc
    10261 gcgaaggcgc cttcgacggc ttgagcagat caaattcagt ctcccagtaa cgcaatacat
    10321 aaggctcgac gccgacaatt tcgctgactt ctccgatctt gaaatagatt ttatccggca
    10381 acgatgtcgt catctattca gcttcggctg gtgaagattc cgtcggcgat gacatgccat
    10441 taaggctctt cttgagaatc tgactagctt tgaatgttag gactttgcgt ccgacgatga
    10501 tgatttcatc gccggtttgc ggatttcttc ccttgcgggg gcgtttcgtg ttcacaacga
    10561 aattgccgaa gccggagatc ttaacttttt gaccctgttc gagacaacct ttgatgatgt
    10621 cgaaagtggc ctcaacgaca tcggtggcgt cctttttaga gaatccgact ttctcataga
    10681 ttcgcgtgat gatttccgcc ttggtcattg cagccatccc tttcctcgca gttcggttta
    10741 gcttcgcctg tgcgcttgca aaagcttgcc gatgtggtcg agcacctgtt gatgcatcgt
    10801 atttacctcg ctgtccgtta aagtccggtc gtcggcacga taatagattc tgtatgccaa
    10861 gctcttcttg ccatgcggaa tcgaatcgcc ccgatattcg tcgaaaatct ctattccttt
    10921 aatcagactt tggcccagtt cgtttataca actgatgatc ttttgagacg aaacagcctg
    10981 atcgacaact attgcaaaat cacgctcaac cgccggaaaa cgcggcaggg aactagccct
    11041 tatttgccgc ggagaatact gaagcaactt ttcaaagtca agttcgaatg ccagcagcgg
    11101 cggcaggccg aattgatcgt taagatcagg gtgactctcg cccacgtacc ctatcatctc
    11161 actatcgtgc tctagttgag cgctgcgtcc gggatggaga acggtgacgt tggcgcgtga
    11221 ccaaaggatc gattctcgta aatggaatag atcgagaatc ccttcaatga ttcctttaca
    11281 atcaagaaat ctaactggcc ggagtccgtc ggacgaccgc aatcctttgc gcctccgcga
    11341 cccgaagagc agccccccca agcattgctt ctcttcaatc tccccattgg cgtgcaatcg
    11401 aaaaaccttc ccgagatggt aggcgaagaa cccctccgct ttttgcgcga gattcaggcg
    11461 taagttctcg atcaatccgg gcagtaagct gaggcgcatc tcggcgctgt ctttgacgag
    11521 cggattcaaa accgccaccg gagaggttgg accgttccat aggcccgtaa acatttgatt
    11581 cagtgtctcg tttgtaaacg ggagattgat cacttcgacc aaaccttcac ccgccagaaa
    11641 cgagcggagc ctgcgttccc acatcagttg ataatcagcc ttgccggccg tcgaacgcga
    11701 aacgggcagc gtcgtcggaa ttttctggta gccaaaaagg cgagctagct cttctattac
    11761 atcggcttca cgggtcaaat cgccacggct cgtcggcggc acaaccatca aactatcctc
    11821 gcccgcggct ttcttggcgt taagaccgag agccgtcaga atcctttccg cctctaccag
    11881 tgcaatttca accccgagca ctcttttgat gcggtcctgc cgcagtaaaa tcgatggagg
    11941 ttgcggcggg ttggggtagc tgtccagaac cccggacgaa accgaaccgc cggcctgact
    12001 ggagataagt tgagccgcgc gattacccgc cgacacggtc cccgccggat cgacaccacg
    12061 ttcgaaccga tgcgatgctt cactatgcaa tcccaaacgt tttgctgtgc gccgaatcgt
    12121 agtcggatcg aaatgggcgc tctccagaag cacccggcga gtcgcggcag tgacctcgga
    12181 attttgcccg cccatgatgc cggccaaagc gacgggcgtg tttccatcgc agatcagaag
    12241 atcgccatca gcgagttctc gcttcacacc atcgagcgcg acgaactctc tttcgccgct
    12301 ggcctgccgg atcacgatgt gatcgctggt caatcggtca agatcgaaag catgcagcgg
    12361 ttgacccact tcgaccatca cgtaatttgt tacatctacg acgttattaa ccgagcgaaa
    12421 accgcaggcc tcgaggcgat gctgtatcca tttcggactt tgactgatgc ggagattatc
    12481 aattacaaag gcagagtagc gcgagcagag ttcgggatta acgattttga tcccaacgtt
    12541 tcctaggtaa ggcgtcactt tggcttgcac gtcgctgcgg cgattcttta gcggcttgtc
    12601 ggtgagcgcg gcaacttcac gcgctaggcc taagatgctc aggcaatcgc cgcgattggg
    12661 cgtcacactg atttcgaaga cccagtcgtc ggcatcggcc tccgatccag tcagcggagc
    12721 gagcgattcg acttcaagtc cggccatcgt cagcaattcg gaaagttcat tggcagaagc
    12781 atctatctcg accaactcct tcaaccaatt aaaagtgaat ttcatgagaa tcgcataacg
    12841 accggaatct ccgagccaat tcttcagccg aactgccgca ggaatctcat atcgttctgg
    12901 aagaataggc gaatgtcatc gatgccatat ttcagcattg cgatacgctc gactcccata
    12961 ccgaaagcga acccggatac tcgctctgga tcgtaaccga cgaacttgaa gacctccgga
    13021 tcgatcatac cggcacctaa gatctccagc cagccgctgc ctttacaaac tctgcagtgc
    13081 tgctgctgag cggcatatcc gtcaccgccg caaatcacgc attgaatatc gatctccgcg
    13141 ctaggctctg tgaaggggaa gaaactcggt cgaaaccgca caccggcttt ctcgctaaac
    13201 atacgctgca aaaaatgcgt cagcacccct ttgagatccg agaaggcgat atcgcgatcc
    13261 accataaagc cttctacctg atgaaacatc ggcgaatgag tagcgtcgtc gtcgtggcgg
    13321 taaaccgcgc ctggaacaat cacctggagc ggcggttggc ggctctccat ggtgcgaatc
    13381 tgcaccggtg aagtatgggt cctcagcagt cggccgtcag aaacaaaaaa agtatcctgc
    13441 atatctcgcg caggatgatc tttggggatg ttcagcgctt cgaaattgtg ataatcgtcc
    13501 tcgatatcag gaccgcgggc aatctcaaag cccattcccc caaaaacatc gataatctca
    13561 tcgatcacca atgtgagcgg atggatgcgc cccgattccc agcgatttcc cggcagcgtg
    13621 acatcgatgg attcttcccg tagcgctttt tctttctgct gccgcttgac gacctgaagc
    13681 ctctcctgaa gccggctttc gagtaattgc ctcagcgcgt tcaatcgctc gccggcgctc
    13741 ggcctttggt ccaagggaag atctttaagt ccccgtaaaa gcagggtaag tcttcccttg
    13801 cgccctaaag cttcaacacg gatctgttca atctccgcct cacttccgga cttctcgatg
    13861 cgcgccagta tctggtcacg aagtgaatct aaagaatcga tcatctagac agaagcgatg
    13921 ggtttaagca tcgaggctgg atttggctcg ctgagctagt tggttaaatc cgtccatgtc
    13981 ggcaactgcg atttcggcca ggacttttct gtcgatttcg acccgggcct gtttcaaacc
    14041 tttgatcaat tgactatagg atagcccatt gagctttgcc gccgctccga tacgcgtaat
    14101 ccacagaccg cgaaaagtcc gtttcttcac gcgccgatcc cgataagcga atgctaaagc
    14161 tctctcgact gtttcgcgcg ccgaccgata cagccggccg cggcctccca cataaccctt
    14221 ggccattttc atgacctttt ttcttctctg ccgggttttg ctcccgcctt tagctcgtgg
    14281 caattgctag ctcccttcta aaatcgtgtg gaattaaaga caaacctatt tgtacgggat
    14341 cagctcccga atgtttctct gttcagctgc cgaaaccatg gtaccgctgc gcagttgacg
    14401 cttacgcttc tttcccttgg aagaaagcaa gtgtctcttg aagcctttat tgcgtttgat
    14461 ttttccgctg gccgtcactc gaaacctttt ggccgcagct ctgttggtct tgattttggg
    14521 cacatcaatc tcctctatga attagttgga taaattactt tggcgtaagc agcatcgcca
    14581 tgttccgccc ttccaagcgc ggctcgatat ccactttggc aatgtcttgt atctgctcga
    14641 ccactttgcg caacgcctgc cggcccagct ccggatgagt aatctcgcgg ccccgaaaaa
    14701 atactgaaac tttgactcgt tggcccttct cgataaattg ccggatgtta cggaccttgt
    14761 gttcaacgtc gtgcgcgtcc gttcgcgatc ccattttaac ttctttaatt tcaaccacag
    14821 tgtgcttttt cggtccgtga gtcttcttcc tttgctggta ccggaacttc ccatagtcca
    14881 tcatacgaca gacaggcggt tgagcttctg gggcgacttc gaccagatct aaacctagag
    14941 catcgacttg ccttaatgcc tcgtgcagcg gcagaatgcc gagctgttct ccttgcgctc
    15001 cgatgacacg aacctcacgg gcccggattt gatgattaat tctcgcgtct ctagcgataa
    15061 gacacctcca tgatgtaaaa gccgctcata tccttatgcc tctccccttt cctgtgcaac
    15121 ttcagatctt agtctctcca tgaattcgtt caaagacatc ggcgctagat tttctccgcc
    15181 tctgcgccgc ggcgccaccg tctgggcttg aacctcgctg tcgccaatca caatcgcata
    15241 cgggattttg gcgagctggg cttcgcgaat cttataacca ag
//
LOCUS       DASUVS010000451        11397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1732293, whole genome shotgun sequence.
ACCESSION   DASUVS010000451 DASUVS010000000
VERSION     DASUVS010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11397
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732293"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1067)
                     /locus_tag="VF182_23785"
     CDS             complement(3..1067)
                     /locus_tag="VF182_23785"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6440158.1"
                     /translation="MHRLSKRFGANEILKDISFEVGDGEFCILLGPSGCGKSTLLRLI
                     AGLEKPSSGEILFDGQHIEHLPPRERNIAFVFQSYALYPHMTVFDNLAFSLKLRGEPA
                     TEIAAKVRDAARLLEIENLIERRPHSLSGGQRQRVALGRAIVRQPQIFLFDEPLSNLD
                     AALRATMRVELARLHRRLGTTIVYVTHDQAEALTLGEKIIVLHQGIIQQIGSPSDIYQ
                     RPVNTQVASFVGNPQMNLLHGTVDENGSVFQCGSLRLNLATIFGRSVPQLAGNSVAIG
                     IRPEDLQLAEAAGQSWISGDVEIIEDLGSDRFLHVKCGDIELIARTGRESTVKQGDRV
                     GLSVAPEQVHIFKDDKRFSP"
     gene            complement(1312..2127)
                     /locus_tag="VF182_23790"
     CDS             complement(1312..2127)
                     /locus_tag="VF182_23790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6440159.1"
                     /translation="MKRSLTLTLFALAIALYCLLPFLWFVLTSLKTPAELASIPPSLV
                     PSLHWGFYHSALFERGLLRYIGNSLIVAGAATVISISVGSMAAYAISRFQLPWTKFYL
                     LALLAISMFPQIAIAGPVWNILNHLDWLNTYHGLVTAYIALALPLAIWILTTFFREVP
                     VEIEEAALIDGCSRLQVLRKVVLPLAAPGILTAALLVFIQIWNEFFFALIIMTDPGVQ
                     TLPVGIALFPGEYTMPWGEIAAASTLATLPLILLTLFFQRGIVRGLSAGAVKG"
     gene            complement(2124..3011)
                     /locus_tag="VF182_23795"
     CDS             complement(2124..3011)
                     /locus_tag="VF182_23795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6440160.1"
                     /translation="MKSSHRLALLFIVPSLVFVCVFTLYPIVESFRLSFYRMILTLPW
                     LGQKMVGWENYVDLWTDPVAWQALRTTIIFVAITVPSELLLGLGMALVMNEAFRGRGV
                     LRAIVLVPWAIPTVVSSQMWRFIFNDRYGLFNFVLFGGDTARYLAPLADPRFAMFAIM
                     AAEIWKTAPFAALIILAGLQTISDEVYEAASIDGATAWQKFHHVTLPLLWPALLLALL
                     FRTIDALRVFDLVFVMTQGGPADATNVLQFYGYKKTFAEGMIGYGSTIAVTVFMISLV
                     LSLAYLRALKSERLQGAVR"
     gene            complement(3008..4300)
                     /locus_tag="VF182_23800"
     CDS             complement(3008..4300)
                     /locus_tag="VF182_23800"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440161.1"
                     /translation="MSRIFMRELFCIFVFVVFLLPQSVRAQATLLRIVISKTDTPQLW
                     DGAIADFEKQNPGVKVVTEIAPNSSTQFHDLLTQKLKNRDTRLDVFFMDVIWPSEFAS
                     AGWTLPLDGFFSTTEQGKFLEAPIAANRYRGEIFGVPLFIDAGLLYYRKDLLGKHRMA
                     PPRHWPELVEQAKAILAAERDPHLIGYSGQFKQYEGLVCNMMEFILGNGGALWDEQRL
                     VSALQSDRAKQAVRFVRDHIIGEISSRGVLAYEEPESVALFAQGRAIFHRNWPYAWRV
                     ANDPEQSRVVGQVGMMPLPPFRGGKGASTLGGWQLGISRFSKIPELAWRFVAFMTSHE
                     MQKRIAFSTGRAPTRKALYDDPELLAKIPWLESLLDTFKQAVPRPTTPVYVPLSNIMQ
                     RYFSAALVLPESKIDQRAALAARDMDRVLDLLRETAAR"
     gene            4604..4822
                     /locus_tag="VF182_23805"
     CDS             4604..4822
                     /locus_tag="VF182_23805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440162.1"
                     /translation="MNTVKIIGVVLIVAGVLGLAYGSFSFTKETHEAKIGPLELQVKD
                     KETVNIPAWAGVGAVVIGAVLLLVPLKR"
     gene            4924..5886
                     /locus_tag="VF182_23810"
     CDS             4924..5886
                     /locus_tag="VF182_23810"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440163.1"
                     /translation="MISRVTWFALGFLVVLHSVKLPQAAARIVVGVSTVNVAFLPIYI
                     TQDKGFFKDEGLDVLVVMFNAGATNLQAVIGGDVQVMAGGVPETVLARASGLDIKNFW
                     AISNVMPFQIYGSPKMKSLKDSKGKKFAISRFGSLSEFLTRSALRQAGIDPKDVTMLQ
                     IGATPARFTTLATGMVDATILWFPVTERAKSVGMNKLFDLKDLYPNWTNVGFIAREGW
                     LAKEKEQTAKFLRAYQRGVKHTRENRDDGIRALRKYVGLDAVEAGAGYDEYRDSFPLD
                     GHILDAGIVATVEQEMETGRLKGRISTSEMIDNSFINTIGKPPR"
     gene            6022..6903
                     /locus_tag="VF182_23815"
     CDS             6022..6903
                     /locus_tag="VF182_23815"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6440164.1"
                     /translation="MTIREITQVGVGVPDREKFEAFARDMLGLPVLRSPDGSISYVRS
                     DQYQHRIAASTAPRPVLRYVTFDVGNADQLGSWESRLSASGIAFRRGTAAECAERQVR
                     DFIEFKDPDGHGLGLAHGFEIDSSPVRYTRDLKVLRLGHVLLTVKDTQRSHDFYTGVL
                     GFRLSDWVYIDENIRLCFLRCNERHHSLAFAPCMPGKTPRLQHAMFEVESLDDVMRSY
                     HFLRNHKAPIGMGPGKHPNCQTVHVYAQTPGGFAVEFGWGQRRLDDATHQPVIYPIGS
                     PVDIWGGDIQSSEFELG"
     gene            6970..7701
                     /locus_tag="VF182_23820"
     CDS             6970..7701
                     /locus_tag="VF182_23820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440165.1"
                     /translation="MKDIEQDLIRTALPYWEAEATIVRRFFRKRPTREDHVFWLKAQL
                     WKELHPVDGYFTGLQKELNDLAASFPDIDKTIDRHQYHSLLEQLTQEFNHYVLMADIL
                     EYLLGHPVSAADTFQLPQEKKLGDIRRAFATSGSPVDKAAVLVTEGGGARMFREGKKL
                     KGGRLEKMIASAMEVIYQDEKNHYKEAASEAARMVQNKQDLMRMKEAVREVSLQRVYM
                     RNEMFKEPLTSEELESCVQSHTPSR"
     gene            complement(7989..8729)
                     /locus_tag="VF182_23825"
     CDS             complement(7989..8729)
                     /locus_tag="VF182_23825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX6440166.1"
                     /translation="MRRLFLVYLVFGVLAPGTNAVWAQTSPSAMPASRGPSFEEYIAA
                     FAAADREQTPPTGGILFVGSSSIRLWNDLEKQFGPSIVKRGLGGSRMSDCTRYLDRLV
                     FPYKPRHVVVYAGDNDLAEGIPPEDILISFQKFVDGVQRELPTTRISYISIKPSPARA
                     RLIPKIRETNHLIEKYTSENEKLDFIEVFTAMLDAKGQPRADLFRADELHLNQAGYTL
                     WRKIIAQHVRLSPADSSPVSTALSGSSN"
     gene            9155..10903
                     /locus_tag="VF182_23830"
     CDS             9155..10903
                     /locus_tag="VF182_23830"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6440167.1"
                     /translation="MFENRLIEPEQLLKLLETAVRQAKEAVLITSARLDPPGPQIVFV
                     NPAFSEMTGYNQEEILDKTPRILQGPKSDRAMLQRLREALARGEPFSGETVNYRKDGG
                     EYYVEWDITPIRDSGGQINHFLSIQRDVTERKSAENRLTELLCQIERSRNDLDSILNA
                     LLIGAVMADENGRVVFTNTAARHLFATDGLGVAWQDLFGLNSEDSHHFKLLTQKPAEK
                     RGRNPLQFDRSDGRRIWLEVDVKDDPRDVRGKIFFLYDVTEVHDLRRLLDHRAHFHDL
                     LGKSKAMQQVYQQIRDLAQVDATVLIEGETGTGKELVARAIHASSHRKDSPFIAVNCA
                     GLTESLLSSQLFGHRRGAFTGAIDDQQGFLEAANRGTLLLDEIGDIPIAVQNQLLRVL
                     QEREIVRLGESRPRKIDVRVLAATHRNLSDEAAKGNFRSDLFYRIRVARITLPPLRDR
                     REDIPLLTASFLAQLSAASGKRVTEVSDDALRLLTGYPWPGNVRELRSAIEFAVIRCR
                     GTVIQGDDLPPEIFEPANLGGVVRDDPSSDERARLLDALGRSRGNRARAARLLGISRA
                     TLYRRLSDLKISPEEK"
     gene            11119..>11397
                     /locus_tag="VF182_23835"
     CDS             11119..>11397
                     /locus_tag="VF182_23835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX6440168.1"
                     /translation="MVLALTLGVTDLAKAESIDKDIVDVAASAGSFKTLIAAVQAAGL
                     VDALKGQGPLTVFAPTDEAFAKLPAGTVENLLKPENKDKLTAVLTYHVV"
BASE COUNT         2683 a         3075 c         3259 g         2380 t
ORIGIN      
        1 ggttatggcg agaatcgctt gtcatcttta aagatatgca cctgctcggg cgcgacgctg
       61 agtccgaccc tatctccttg tttaaccgtc gactctctgc cggttcgagc gattagttcg
      121 atatctccgc atttcacatg aaggaaccgg tccgagccga gatcttcgat gatctcgacg
      181 tcaccactga tccagctctg tccagccgct tcggctagct gcaaatcttc cgggcgaata
      241 ccgatagcaa cagagttgcc ggccagttgc ggcacggatc tgccaaaaat tgtggcgagg
      301 ttcaggcgca ggctgccgca ctgaaaaacc gatccatttt catccacagt cccgtgtaac
      361 agattcatct gcgggttacc gacaaatgat gcgacctgcg tattaaccgg ccgttgataa
      421 atgtcagagg gcgagccgat ctgctggatg atcccttggt gcaggacgat aatcttttca
      481 cccagggtta gcgcctcggc ctggtcatgg gtgacgtaga caatcgtggt gccaaggcgc
      541 cggtgcaatc gcgccagctc gacgcgcatg gtggcgcgca acgcagcatc gagattcgac
      601 agcggttcgt caaacaaaaa aatctgcggc tggcgcacaa tcgcacgtcc cagcgcgact
      661 ctttgccgct gcccgccgct caaactatgg ggccggcgct cgatcaagtt ttcgatctcg
      721 agcaggcgcg cggcatctct gaccttggcg gcgatctccg tggcaggctc gccgcgtaac
      781 ttcagagaaa aagccagatt gtcgaaaacg gtcatgtggg gataaagcgc gtagctttga
      841 aacacgaagg cgatgttgcg ctcgcgcggc ggcagatgct cgatatgttg gccgtcgaaa
      901 agaatctctc cggagctggg tttttccaag ccggcgatca gccgcaggag agtagattta
      961 ccgcaccctg aaggtccgag cagaatgcaa aactcgccgt cgccgacctc aaaggagatg
     1021 tccttcagga tttcattggc gccgaaccgc ttcgagagcc gatgcacggt tagagctgcg
     1081 atagggattc ctccgattgt ggaactccgc cggcgcgaag gcgatgagcc ctccagccgt
     1141 gggcaatagg acactgcctc gaggaccacc ccgcaatccc cctcagggcg atgctccgta
     1201 tgttgggcct gccatcaacc tgtacaccca ggtgcccaag gcttccggcc tcaccgcctt
     1261 cgctcgctaa gtcagccgca ggttttgtcg gatcagggta cattttcgct ttcaaccttt
     1321 tacggcgccc gccgacaagc ccctgacgat cccacgctga aaaaacagcg taagcaatat
     1381 cagcggcaga gtcgccaagg tcgaagccgc ggcgatttct ccccagggca tggtgtattc
     1441 gccgggaaag agcgcgatgc ccaccggcaa cgtctgcaca cccgggtctg tcatgatgat
     1501 cagagcaaag aaaaactcat tccagatctg aataaagacc aacaacgccg ctgtcagaat
     1561 tcccggcgcc gctagaggca aaaccacttt gcgcaaaacc tgcaaacggc tgcaaccatc
     1621 gatcagcgcc gcctcctcta tttcaaccgg gacttcgcga aaaaatgtcg tgagaatcca
     1681 aatcgccagg ggcagagcaa gcgcgatgta agcggttacc agaccgtggt aagtattcag
     1741 ccagtcgaga tgattaagaa tgttccagac cgggccggca atggcgattt gcggaaacat
     1801 cgaaatcgcc aacagcgcca gcaggtagaa ttttgtccat ggcagttgaa aacggctgat
     1861 ggcataggcg gccatagagc ccacgctgat gctgatgacg gtggcagcgc cggcaacgat
     1921 cagactattg ccgatgtagc gaagcaagcc ccgctcgaac agcgctgagt ggtaaaatcc
     1981 ccaatggagc gatggcacga ggctgggcgg aattgacgcc agctcggccg gagtcttcag
     2041 cgacgtcagg acaaaccaga gaaacggaag caaacagtag agggcgatcg ctagagcaaa
     2101 gagcgtcaat gttaaagatc gtttcatcgc accgctcctt ggagtcgctc cgatttgagc
     2161 gcgcgcaagt aggccaacga caaaaccagc gagatcataa aaaccgtcac agcgatagtg
     2221 gagccgtaac cgatcatgcc ttcggcaaaa gttttcttat atccgtagaa ctgcagtacg
     2281 ttagtggcat cggcggggcc gccctgggtc atgacgaaca ccagatcgaa gacgcgcagg
     2341 gcgtcgatgg tgcggaaaag cagcgccagc aatagcgccg gccaaaggag cggcaaagta
     2401 acgtggtgaa atttctgcca agcggtggcg ccgtcgatgc tcgcggcttc gtaaacttcg
     2461 tcggaaattg tttgtagccc ggccagaata atcaaggccg cgaagggagc ggttttccag
     2521 atctccgcgg ccatgattgc aaacatggcg aatcgcgggt ccgccagcgg cgcaagataa
     2581 cgcgctgtat cgccgccgaa cagcacgaag ttgaacaggc cgtaacgatc attgaaaata
     2641 aagcgccaca tttgtgagga gaccacggtg ggaatagccc acggaacgag gacgatggcg
     2701 cgcaacacgc cgcgcccgcg gaacgcctcg ttcatcacca gcgccatgcc aagcccgagc
     2761 aacaactccg atggaactgt gatcgcgacg aagatgatcg tcgtccgcag cgcttgccat
     2821 gccaccgggt cggtccagag atcaacatag ttctcccagc cgaccatctt ttgcccgagc
     2881 cagggcaggg tcaagatcat tcgataaaag ctcagacgaa acgattcaac aattgggtag
     2941 agcgtaaaga cacagacaaa gaccagactg ggaacgataa atagaagcgc caagcgatgc
     3001 gagcttttca tcgggctgca gtttcgcgca ataagtcgag cacacgatcc atgtcgcgcg
     3061 ccgccaatgc cgcgcgttgg tcgatcttgc tttcgggcaa gaccaaggca gcgctaaagt
     3121 aacgttgcat aatatttgac aacggcacat agaccggcgt tgtcggccgc ggtaccgctt
     3181 gcttgaaggt atccagcaga gattcaagcc atggaatctt ggccagcagc tcggggtcgt
     3241 cgtaaagcgc ctttcgggtc ggcgcgcggc cggtggaaaa ggcgatccgc ttttgcatct
     3301 catgactggt catgaatgcg acaaagcgcc aagcgagctc gggtattttc gaaaaccggc
     3361 tgatacctag ttgccagccg ccgagtgtcg aagcaccctt gccaccgcga aagggcggca
     3421 atggcatcat gcccacttgc ccaaccactc tcgattgttc gggatcattg gcgacgcgcc
     3481 acgcataggg ccaattgcgg tgaaaaatcg cccgtccctg cgcgaacagc gcaacggatt
     3541 ccggctcttc gtaggccagc acaccgcgtg atgagatctc gccgatgata tgatcgcgca
     3601 cgaaccggac cgcctgcttg gcgcggtcgg attgcagcgc gcttaccaat cgctgttcat
     3661 cccataaagc gccgccgttg ccgaggataa attccatcat gttgcagacg agcccttcat
     3721 attgtttgaa ctgccccgaa tatccgatca ggtgcggatc gcgttcggca gcgaggatcg
     3781 ctttagcttg ctccacaagc tcgggccagt gacgcggcgg cgccatccga tgtttcccga
     3841 gaagatcttt tcggtaatag aggagtccgg catcgataaa aagcggcacc ccgaaaatct
     3901 caccgcggta gcgattggcc gctatcggcg cctcgagaaa tttgccttgc tcggtggtgg
     3961 aaaagaaacc atccagcggt aacgtccacc cggccgatgc aaactcgctc ggccagatga
     4021 catccatgaa gaagacgtcc aggcgggtat cgcggttctt caatttctgg gtgagcagat
     4081 catggaactg cgtcgaagaa tttggcgcga tctcggtgac gactttaacg ccagggtttt
     4141 gcttttcgaa atcggcaatc gccccgtccc aaagctgcgg cgtgtccgtt ttagagatga
     4201 cgatgcgaag gagtgttgcc tgcgcccgaa ctgattgcgg tagaagaaaa acgacgaaga
     4261 cgaagatgca aaaaagctcc cgcatgaaaa tccggctcat taattcatcc cggttcgtta
     4321 aatcgcgctc taattatgca ggggcagcct ataatctgat gtcggtcatg tccagcattt
     4381 gccggccgta aaataatctc tcggttctgt agtaggaagg gaactcggga accgcttttg
     4441 tagtatggga cgaggatctg gtgttttgaa aggttggcag ccgtgtgccg ccggccaatc
     4501 gagagtgtta agcggggatg ttgacaaatc tccgcagatt cgaatagaga gaatcgcacg
     4561 catcgctagc aacgatacac gagatcgttt taggaggcat gagatgaaca cagtcaaaat
     4621 aattggagtg gttttgatcg tggccggcgt tctcgggctg gcctatggca gtttcagctt
     4681 cacaaaagaa actcatgaag ccaagatcgg gccgctcgag cttcaggtga aagataagga
     4741 aacggtcaat attccagctt gggctggtgt gggagcagta gtgattggcg cagtcttgct
     4801 cctggtgccg ttgaagagat aattgggaat cgcacatacc ctccagcagt cagatgctag
     4861 agatgtcacg gaaccatcga tgatttgcga tgactaaccc aagagcgcag cggaggtagg
     4921 tttatgatct cgcgggtaac gtggtttgct ttagggtttc tggtcgtact gcatagcgtc
     4981 aagcttccgc aagccgcagc tcgcatcgtt gtcggcgttt cgacggtgaa cgtggcattc
     5041 ctgccgatct acatcactca ggacaaaggc ttcttcaagg acgaaggtct cgacgtgtta
     5101 gtggtgatgt ttaatgccgg cgctaccaat ctccaggcag tcatcggcgg cgacgtccag
     5161 gtgatggcgg gaggagtgcc ggagaccgtg ctcgccagag ccagcgggct ggacatcaag
     5221 aatttctggg cgatctccaa tgtcatgccg tttcaaatct atggcagtcc caagatgaaa
     5281 tcgctgaagg acagcaaagg gaaaaagttt gcgatcagcc ggtttggctc attgtctgaa
     5341 tttctcaccc ggtcggcgct gcgccaggcc ggtatcgatc ccaaggatgt cacgatgttg
     5401 cagatcggcg ccacgccggc gcgcttcacc acattggcca ccggcatggt cgatgcaacc
     5461 atattatggt ttccggttac cgaacgggcc aagtcggtcg gcatgaataa gcttttcgac
     5521 ctgaaggatc tctatcccaa ctggaccaac gtcggtttta ttgcgcgcga gggctggctc
     5581 gccaaggaga aagagcagac cgccaagttt ttacgcgctt atcaacgggg cgtgaagcat
     5641 acgcgcgaga accgcgacga cggcattcga gcgctcagaa agtatgtcgg gctcgatgcc
     5701 gtcgaggcgg gcgccggata cgacgagtac cgcgactcgt ttccgctcga cggccacata
     5761 ctcgacgcag gtatcgtcgc aaccgttgaa caggagatgg aaaccggcag actgaagggg
     5821 cgaatctcta ccagcgaaat gatcgacaat agcttcatca ataccatcgg caagccacct
     5881 cgctgattcg ataatttctt gacagcaatc tgcctggagc gatagtttcc ggttgctggc
     5941 ggaggatgag aggattcgtt ctgacctctc atgcgttaag gagcgggatc ttaagcgtcc
     6001 tctcactaaa ggaacctact aatgacaatc agagaaatta cccaggtcgg tgtcggtgtg
     6061 ccggatcggg aaaaattcga agcctttgcg cgcgatatgc tcggcttgcc ggttctcaga
     6121 tcaccggacg gaagtattag ctacgtccga tcggatcagt atcaacaccg gattgccgcc
     6181 agcacggctc cgcggccggt tctacgctat gtgacttttg acgtcggtaa cgccgatcag
     6241 cttggcagct gggagagccg gctcagcgca tccggcatcg cgttccgccg cggtacagcg
     6301 gcagagtgcg ccgagcgaca ggtgcgcgat tttatcgaat tcaaagatcc cgacggacac
     6361 gggttaggtt tggcgcatgg tttcgaaatc gattcttctc cagtgcgcta cactcgcgat
     6421 ctcaaggtgc tgcgcttggg gcacgtgctg ctgacggtaa aagacaccca gcgctcgcat
     6481 gacttctaca ccggtgttct gggctttcgc ctgagtgatt gggtttacat cgacgagaac
     6541 attcgtttgt gttttttgcg ttgcaatgag cgccatcaca gcttggcttt cgcgccgtgc
     6601 atgccaggca agaccccgag gttgcaacac gcgatgttcg aagtggagtc cctcgacgat
     6661 gtcatgcgtt cgtatcattt cctgcgcaat cataaggcgc cgatcggcat gggacctggc
     6721 aagcacccca attgccaaac cgtgcacgtt tacgcgcaga cgcctggcgg ttttgccgtg
     6781 gagtttggct ggggccagcg ccgtctcgac gatgccactc atcaaccggt gatctatccg
     6841 attggcagcc ctgtcgatat ctggggcggc gacattcaaa gttcggagtt cgagcttgga
     6901 tagaaccgcc aaatttcgat gattgaaagc cgtgagatgt ccatcgacca cgtgtcaaga
     6961 aagcgagaca tgaaagacat cgaacaagac ctcatccgca cggcgctacc ttactgggaa
     7021 gctgaggcta cgatcgtccg gcgatttttc aggaaacgac cgacgcgcga ggatcatgtt
     7081 ttttggctca aggcgcagct ctggaaggag ctgcatccgg tggacggtta ttttaccggg
     7141 ctgcagaagg aactgaacga tctggcggcg agttttcccg atatcgacaa aaccatcgac
     7201 cggcaccagt accactcgct tctcgaacag ctcacccaag agttcaatca ctacgtgctg
     7261 atggccgaca ttctcgaata tctgctgggc catccggttt cagcggcgga tacttttcag
     7321 ctgccacagg aaaaaaagct cggtgatata cgcagagctt tcgcgacttc gggctcacct
     7381 gtggataaag ctgcggtgct cgttaccgaa ggaggcggcg cccggatgtt tcgcgaagga
     7441 aaaaaactca aaggcggccg gctcgaaaaa atgatcgcca gtgcgatgga agtgatttat
     7501 caggatgaaa agaaccatta caaggaggcg gcaagcgaag cggcacggat ggtccagaat
     7561 aagcaggatt tgatgcggat gaaagaggcc gtgcgtgaag tgagcctcca acgagtgtac
     7621 atgcgcaatg aaatgttcaa ggaaccgctg accagcgaag aactcgaaag ctgcgtgcaa
     7681 tctcacactc cttcccgcta aagcggtccg aaatgcgcaa acgagggctg catttctgtt
     7741 ggcccccaag gatcatcgct atgacagcct gtctaacggt gccgtgcagc tcgagtcgat
     7801 cgctttacag atacctcggt tatagcaaaa ctaaccctcc gagacgtatg cgagaggctt
     7861 acatcaagcg cgttttgctc actgagttgt tgttctgctg gtgcttgcgc cattgacgaa
     7921 ggcgaagagt gtgaatgtcc cgtcgattgc cggaagcttc gcggcgctgt atggagtcgg
     7981 ccgagtcgtt aattcgatga gccacttaga gcggtagaaa cgggggagga atccgccggg
     8041 ctcaaccgca catgttgcgc gatgatcttt ctccacagcg tgtaaccggc ctggttcagg
     8101 tgcagctcat cggcgcgaaa gagatcggcg cgtggttgcc ccttggcatc gagcatcgcc
     8161 gtgaacactt cgatgaagtc cagcttttca ttctcggagg tgtatttttc gatcaaatgg
     8221 ttggtctccc ggatcttggg aatgagccgg gcccgggccg gactcggctt gattgagata
     8281 taggagattc ttgtggttgg gagctctctc tgtacgccgt cgacaaactt ctggaagctg
     8341 atcaggatgt cctccggcgg tattccctct gccaaatcat tatcgccggc gtaaaccact
     8401 acatggcgcg gcttgtaagg gaaaaccaat cgatctaggt aacgcgtgca atccgacatc
     8461 ctggagccac ccaaaccgcg cttaacaatc gacggcccaa attgcttttc gagatcgttc
     8521 cacagcctga tcgacgaact gccgacgaat agaattcctc ccgttggcgg ggtctgctcc
     8581 cggtctgcag ccgcgaaggc ggcaatatac tcttcaaacg atggccccct ggatgctggc
     8641 atggccgaag gcgaggtttg agcccaaacc gcgtttgtgc ccggtgcaag gacgccaaat
     8701 acaagataga ccaaaaacaa tcttcgcatg aagttatccg ttgcgccttt tcgaaccgat
     8761 cgaactcagc ctcggttaaa ctgtatcgct tccggaattg actggccatt taaaccctca
     8821 gctcgccatt cctaggtctg gaatatcctc aattctgccg cctaagtcaa tcaccgcagg
     8881 gcggccgatc ggccgagcag tccagcgtgc taaatggcaa gcaaaaggcc gccggcacta
     8941 gccgtttcgc atcaggtcca gccacatgaa ttcacgatca tagccaatca gtccccttgc
     9001 attttcgaca tccgcagaag ccgtgagcta agttggagat tgatccgctc ggaagttatt
     9061 gccagttgca gatcgaagat gttagttcga ggattagcca acgggcacga taagggtttg
     9121 agtcaaccga gaccaaagcg ctaggagaca atctgtgttt gaaaaccgtc tgatcgagcc
     9181 ggagcagctt ctgaagttgc ttgagaccgc cgttaggcag gccaaagagg cggtgctgat
     9241 cacgtcggcg aggttggatc cgccggggcc gcagattgtt ttcgttaatc cggccttcag
     9301 tgaaatgacc ggatacaatc aagaagaaat cctcgacaaa acaccgcgga ttcttcaggg
     9361 tccaaagtcc gatcgcgcca tgttgcaaag gttgcgcgag gcactggctc ggggcgagcc
     9421 gttttccggc gagacggtca attaccgtaa ggacggcgga gagtattatg tcgaatggga
     9481 tatcacaccg attcgcgact cgggcgggca gatcaatcat tttctttcga tacagagaga
     9541 tgtcaccgag cgcaaatcag ccgagaaccg gctcacagaa ttactttgcc aaatcgagag
     9601 aagccgcaat gatctggatt caatcctcaa tgcgctcctg atcggcgcgg tgatggccga
     9661 cgaaaacggc cgagtggtgt ttactaacac ggcagcccgg catctttttg ccaccgacgg
     9721 tctcggcgta gcttggcagg atctcttcgg cttgaactcc gaagatagcc atcatttcaa
     9781 gctgctgaca caaaaaccgg ccgagaagcg gggacggaat ccgcttcagt tcgaccgcag
     9841 cgacggccgg cgcatctggc tcgaagtaga cgtcaaagac gaccctcgag atgtccgcgg
     9901 aaaaatcttc tttctttatg atgtcacgga agttcatgac ctgcgtcgcc ttttagacca
     9961 ccgggcgcac tttcacgatt tattgggaaa gagcaaagcc atgcagcagg tttaccagca
    10021 gattcgcgat ttggcccagg tggacgcgac cgtgctgatt gagggtgaaa ccggaacggg
    10081 taaggagctc gtcgctcgcg cgattcatgc ctcgagtcat cgcaaagata gccctttcat
    10141 cgccgtgaac tgcgcgggcc ttacggaatc gcttttgagc agccagctct tcggccatag
    10201 gcgtggagct tttaccggcg ccatagacga tcagcaggga tttttggaag ccgccaatcg
    10261 cggcacgctg ttgttggatg aaattggcga cattcccatt gccgtgcaga accaactgct
    10321 gcgcgtgctg caggagcgcg agatcgtccg tcttggagag tcacgcccgc ggaaaatcga
    10381 tgttcgagtg ttggcggcga ctcaccgcaa tttgagcgat gaagcggcca aaggaaactt
    10441 ccgttccgac ttgttttacc gtatccgggt ggcgcgaatc actttgccgc cgctgcgcga
    10501 tcgtcgtgag gatatcccgt tgctgactgc ttcgtttctc gcccagctct ccgcagccag
    10561 tggaaagcgc gtgaccgaag tgagcgatga cgcgttgcgc ttacttacgg gttacccatg
    10621 gcccggcaac gtgcgcgagc tcagaagcgc aatcgagttt gccgtgattc gctgccgggg
    10681 cactgtgatt cagggcgatg atttgccgcc ggaaatcttc gagccagcga atttaggcgg
    10741 cgtggttcgc gatgatccgt cgagcgacga gagagcgcgt ttgctcgatg ccttgggtcg
    10801 ttcccgcggg aaccgtgccc gggcggcgcg ccttctgggc atcagccggg ccacgcttta
    10861 ccgccgttta tccgacctca aaatcagtcc cgaagaaaaa tagtctcacc tgtttcagtc
    10921 gagacatcgt tgagatcgcg ctgagacatt ttactgtcgc agagcatttt gcgatttcat
    10981 tttccatggc acaacgccag gctaaaccgc cgcaggccaa cgaaattcaa gtggcaccgc
    11041 ggttgctgat gtccagtttc gaagattcac aaaaaaataa aaaggaggaa aaaaatgaga
    11101 gtcttcaaaa ctgcattgat ggtattggca ttaacgttgg gagtcacgga tttggcgaaa
    11161 gctgaatcga tcgacaagga catcgtcgac gttgccgcgt cggcgggatc cttcaagact
    11221 ttgatcgctg cggtgcaagc cgcaggactg gtcgatgcgc taaaggggca gggaccgttg
    11281 acggtcttcg cgccgaccga tgaggcattt gcaaagctgc ccgcgggcac agtggaaaac
    11341 ctgctcaagc ccgaaaacaa agacaagctc acggcggtgc tgacctatca cgtggtc
//
LOCUS       DASUVS010000452        12941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_857577, whole genome shotgun sequence.
ACCESSION   DASUVS010000452 DASUVS010000000
VERSION     DASUVS010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12941
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857577"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1447)
                     /locus_tag="VF182_23840"
     CDS             complement(<1..1447)
                     /locus_tag="VF182_23840"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6440169.1"
                     /translation="MPLAETDKFRTAGWISGSDRSLAGNSQVQQSSASNSLIPNVLVV
                     DDDPTIRSQLVRLYSHNGYTVVPVSSAEEALAQLNEGNIDFVITDIKLPGLSGIDLIA
                     HMQENFPDVPVIAITGFKDIDTAVNVLKHGAADFVVKPFDLGAVQESTRVALEKTRVY
                     MEIRHLRRGLKNGSEFGGMLSKTAEMHRMFEIIRMVAPTEMTVSIEGETGTGKELVAS
                     AVHYHSGRRDKPFVTINCAGFPESLLESELFGYERGAFTGAVQAKAGKIELAHGGTLF
                     LDEIESISVVMQGKLLRVLEDQKVQRLGGSADIHVDMRVITATNVPLKQLVTEGKMRS
                     DFYYRVNVIPIHLIPLQQRKVDIPLLVQDFLHRHPVAARKGITSISKQGMRRLLDYSW
                     PGNIRELQNVLERAIVLNAGRTIEHVDLPEVSFEAHPSKGGPNFNAPLNQWLQEKEKE
                     YLAQKLDDFGGNVGLTAKSCRIGVRTLSRKMR"
     gene            complement(1451..2599)
                     /locus_tag="VF182_23845"
     CDS             complement(1451..2599)
                     /locus_tag="VF182_23845"
                     /inference="COORDINATES: protein motif:HMM:NF020036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX6440170.1"
                     /translation="MSKISTLSRTKMKQTGAHEGSAQPELASRLRAGLIDAGTAIELV
                     PSPAALWSLDRQSCFFNDDARELLGFCEHDFKQDAALWLERIHPDDRKEFLRAWKSLQ
                     AGETRVSSCYRFQPKNRTQVVCLKEVSFLYTPSGVEKPNVWTVYLEEVISDSFLHGAS
                     QMRNFVRGLTHEMGNNLQAIRGELDILRFSQAIPNDSAETIYRGINQVRRLVHEIQEY
                     LLPPSVEATTEDPASVLAEVIQSRAKEMAAHGIRTGMKLKESLPKVPLDLQFGRALTN
                     VIDFSRALLSKGGELNIEAGVCQRDGENYIELNIVSSSETSLQVDEGEVFRPFASVNG
                     HRAGLSMAVAQQILKRHFGKIVFRKEQSNRGVFSLLIRVPSNAEPSKK"
     gene            complement(3022..3516)
                     /locus_tag="VF182_23850"
     CDS             complement(3022..3516)
                     /locus_tag="VF182_23850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440171.1"
                     /translation="MKEPSIEELTAEAMQLLPMGLEELYMILGAQLLVSAKPTRVAGI
                     MTYLSAARKAREAKELYATLPLTPTPADWSAGLESIYNELIRDGIQYIEEVKQELHRG
                     VCNEDIFSLSDKMDSSGMQIVVMIVGAVLKMPPQLENVCAVLAAILYKIGLREFCRSP
                     LGDA"
     gene            complement(3622..4986)
                     /locus_tag="VF182_23855"
     CDS             complement(3622..4986)
                     /locus_tag="VF182_23855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009362447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX6440172.1"
                     /translation="MNYQVEAAIASKEQSITHRSQMGRELKKKIRDRSAKVGVVGLGY
                     VGLPLALEMAQAGFEVTGIDLVRERVDSINSGISYIPDVTGEALLSAVSANRLRATQS
                     LSAAEKVDTLNICVPTPLRKNKDPDLSYVIAAVEAIRTHIRPGQLIILESTTYPGTTR
                     EVVLPILEESGLNVGRDFFLAYSPERVDPGNQNFTTRNIPKVVGGVTPRCAEIAVLLY
                     EQFVEKALPVSSTDCAEMVKLLENTFRSVNIALANEMALTCHSLGIDVWEVIEAAKTK
                     PFGFMPFYPGPGLGGHCIPVDPYYLTWRAKMNGCEPRLIELAGHINSQMPGFTVLGIA
                     DALNERRKCLRGAKILGLGVTYKRDTNDVRESPALHVLEGLRKKGASISYSDPHVSSI
                     SVAGQTINSVELSAELLQSMDCVAVLTDHSSFDYSLIAQYSPLIFDTRNALKDFPSAN
                     SVLL"
     gene            complement(5032..6117)
                     /locus_tag="VF182_23860"
     CDS             complement(5032..6117)
                     /locus_tag="VF182_23860"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX6440173.1"
                     /translation="MSSSHPPLISLLEAISSDREDLALDKFEAITVAWAIRNGLGPLL
                     YRAVRRNPQSVASSHWSSLKSADLTARVVIGEHFQALREIGDACRSDLPPLTLLKGIS
                     ISEQCYPEPHLRLMRDIDFLVAKESLSAVTTALHQLGYRQLSKGGLRYEKHHHLEPFF
                     HQDKQVWVEVHHGLFSTERRAGSAKIFSRENLAAQTRPSRFQGIEVRRLSFELQLVYL
                     ASHWAQDFARVGGLVALVDTIYLLRQAGADFSWEWLLRAVNSSVPATYLYLLLSYIVR
                     YDLVKVAPEIMRELAFSQPSFGCLSLKTMHRMIDDYLVKATEFGPLLSERSVGIIWNT
                     LMLPGPTLSKFALIPVNLSLPRYCRIQ"
     gene            complement(6171..7028)
                     /locus_tag="VF182_23865"
     CDS             complement(6171..7028)
                     /locus_tag="VF182_23865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440174.1"
                     /translation="MNDYQKLVFRIVGCVVLIECRDQAAAELIRANYAAMQIYHCANP
                     DLVYAVWRRNGSQFCVARQGREPITAADEGELLFVLEKELTIELQKIRRELYFIHAAA
                     LSFAGQGFLIAAPSGTGKSTTTWALLHHGFDYLSDELAPVDLSSLRVQPYPHALCLKK
                     EPPLPYSLPAQSVRTCSTLHVPVARLPNSLLAKPALLSALFFLEHCPELKMPTVDAIT
                     KAEAAARLFVNALNPLAHEQDGLAGAAVIAQRVPSFHLKSAALGLTCELVKSILDKSS
                     AANLPESFR"
     gene            complement(7082..7270)
                     /locus_tag="VF182_23870"
     CDS             complement(7082..7270)
                     /locus_tag="VF182_23870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440175.1"
                     /translation="MSETKIKRREFVKRAAYVAPVILSLKAMPAFAASGSGRPRSSKG
                     DPSDSKKETKAPAKNENQ"
     gene            complement(7302..7715)
                     /locus_tag="VF182_23875"
     CDS             complement(7302..7715)
                     /locus_tag="VF182_23875"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX6440176.1"
                     /translation="MGACIWPVLEGWKRANNLDIVSVHARVSDSVNDAILVNLMPQAV
                     TSSKLNELRLRRRKGVQSRLVEGELIILDRDRDLIHQFNKTATFIWEHCDGQQSAAEI
                     ANAVCENFEVDESTAIKEVIETLERLRDLQLIGDD"
     gene            complement(7805..9733)
                     /locus_tag="VF182_23880"
     CDS             complement(7805..9733)
                     /locus_tag="VF182_23880"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX6440177.1"
                     /translation="MLKEITLKYRRIPIVAFHMALIVVANYLAFWIRFDGAIPPQEKE
                     IMLRMIPWLMLIRGLTFVPFQLYKGLWRYTGIWDLRNVIAGVLVSTGLFYITVHWILT
                     AKNYPLSIFIIDSLLLIFLMGGSRLTRRLVYGIGQAKEGRRLLIYGAGDAGEMIVRDI
                     RNNSSFYGYQPIGFVDDDSKKIGRRIHGVQVFGRREDLPRIITEEKPDELVIAIPSAK
                     PAMVREVVKILEPFKIPIRTLPRLSELRNGNVGVKQMRNLAIEDLLDRAPVGLDLEPV
                     HALVKGKRILVTGAGGSIGSELSRQIARYQPAQLVLLDNGESALYDINTELDRSEQSV
                     ERVAVLADIRDARAIEEIFASHAPQLVFHAAAYKHVPMMETHPNEAVLNNVVATHRLM
                     RFAIQYQVERFVQISTDKAVNPTNVMGATKRVAEMCIQALSKSCEQSQTVFTAVRFGN
                     VLGSNGSVVPLFLKQIEQGGPVTVTDPEISRYFMTIPEAVLLVLQAATLARGGEIFVL
                     EMGEQIKLMDMARHLIRLAGFVPEEDIPIHVVGLRPGEKLREELVGMDEMLIPAEVEK
                     IFRVQSAWLSDLQSLTDKIDQMEQMALKGQSRRALALLYETVPTFRPVDPKTANQIRQ
                     PGTNKEPTRAWKLASRSA"
     gene            complement(9979..10929)
                     /locus_tag="VF182_23885"
     CDS             complement(9979..10929)
                     /locus_tag="VF182_23885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEX6440178.1"
                     /translation="MKREVIESKQLPIVVTGGAGFLGSVVVKCLRSRGYEKIFMPRSK
                     EYDLRDQAAITRMYQDFQPRMVIHLAAVVGGIGANRDNPGRFFYDNAIMGIQLMEYAR
                     QFQVAKFVAVGTICAYPKFAPVPFKEEDLWNGYPEETNAPYGLAKKMMLVQAQSYRAQ
                     YGFNAIYLLPVNLYGPGDNFDLNTSHVIPALIRKCMEAKERNEKQIALWGDGSPTREF
                     LYVEDAAEGIVSASENYDGDEPVNLGTGKEILISELAQMIAEEVGFRGEIVWDSTKPN
                     GQPRRCLDTSRAERFFGFRAKFDLRQGIIQTVAWFNAHRL"
     gene            complement(10943..11977)
                     /gene="gmd"
                     /locus_tag="VF182_23890"
     CDS             complement(10943..11977)
                     /gene="gmd"
                     /locus_tag="VF182_23890"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006276645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX6440179.1"
                     /translation="MPKALITGITGQDGSYLAELLLDKGYEVHGVIRRASTFNTERID
                     HLYQDPHINGVRLFLHYGDIADSTNLIKLLYRIQPDEIYHLAAQSHVRVSFDIPEYTG
                     DVSGLGTIRILEAIRETGLKSKFYQASSSEMYGKVQAVPQRETTPFYPRSPYGAAKVY
                     AYWITVNYRESYGLFACNGILFNHESPRRGETFVTRKVTRTAARIKAGLEQKVYLGNL
                     EAKRDWGYAKEYVEAMWLMLQQEEPDDYVIATGQTHSVSELVEEAFSYAGLDWRKHLE
                     VDERYYRPAEVDLLMGDASKAKQKLRWQAKTTFKDLVRLMVDADMVSAKQEAHLNSYN
                     KSNVKAPERL"
     gene            complement(12084..>12941)
                     /locus_tag="VF182_23895"
     CDS             complement(12084..>12941)
                     /locus_tag="VF182_23895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6440180.1"
                     /translation="PLSRDGTVFNWRKKREIYRGSRIYISTPSQWLMSKVEKSMIAPA
                     IVESRVIPTGVDLAIYRSGDKSAARSALHLPQNAKILLFAANQVRRNVWKDYNTLEAA
                     ITLVGKRMELGNIHLVALGENSPDVKIGEAEVHFVPHQANPEAVARYYRAADVYVHAA
                     RADTFPRAVIEALACGTPVVATAVGGIPEQIKSLQSSDSISGSGYSAQEATGILTQAG
                     DAESMALGIERILGNDPLRHQLSRNAKVDAALRFDLQIQVKRYLEWYETILEKPFSTP
                     ENKARISMM"
BASE COUNT         3060 a         3548 c         3272 g         3061 t
ORIGIN      
        1 cgcgcatttt acgagagagg gtgcgcacgc cgattcggca gctcttagcg gtcaaaccga
       61 cgttgccgcc gaaatcgtcg agtttttgcg ccagatactc tttttccttt tcctgcagcc
      121 attggttcaa cggggcgttg aagttagggc cgcccttgct tggatgcgcc tcgaagctta
      181 cctcgggcaa atcgacgtgt tcgatggttc tgccggcgtt gagaacgatg gcgcgctcga
      241 gaacattttg cagctcgcga atgttgcccg gccaggagta gtccaacagc cggcgcatcc
      301 cttgcttgga aatcgaagta atccccttgc gcgcggccac cggatgacga tggagaaaat
      361 cctgcaccaa aagaggaata tccacttttc tctgctgcag cggtatcagg tggatcggaa
      421 tgacattgac ccggtaataa aaatccgagc gcattttgcc ttcggtcacc agctgcttga
      481 gcggcacatt ggtggcggtg atcacgcgca tgtcgacgtg aatatcggcg ctgccaccga
      541 gccgctgcac cttctgatct tccaagaccc ggagcaattt gccctgcatg accactgaaa
      601 tactctcgat ctcgtcgaga aataaagtgc cgccatgggc tagctcaatc tttcccgcct
      661 tggcttgtac cgcgccggta aaggcgcccc gctcataacc aaacagctcg ctctcgagca
      721 gcgactcggg aaacccggcg cagttaatcg tgacaaacgg tttgtcgcgc cggccgctat
      781 ggtagtggac cgcgctggcc accaactctt taccggtgcc ggtttctcct tcgatggaaa
      841 ccgtcatctc ggtgggcgct accatgcgga taatttcgaa catccggtgc atctcggccg
      901 tcttgctgag catgccgcca aactcggagc cgttcttaag accccgccgc aaatggcgga
      961 tctccatgta gacccgcgtc ttctccagcg ccacacgcgt cgattcctgc accgcgccca
     1021 aatcgaacgg cttgaccacg aaatcggctg cgccgtgctt cagcacgttc accgcggtat
     1081 cgatatcttt aaatccggtg atggcaatga ccggaacatc gggaaagttc tcctgcatgt
     1141 gggctatcag gtctatccca ctcaatcccg gcaacttgat atcggtgatg acaaaatcga
     1201 tgttgccttc gttgagctgc gccagcgcct cttcagcaga tgagaccggt acaaccgtgt
     1261 agccgttatg actataaagg cggaccaact gactgcggat ggtcggatca tcgtccacca
     1321 ccagcacgtt gggaatcaat gaattgctgg cgctgctttg ctggacttga gagttgccgg
     1381 ccagggatct atcagaacca cttatccatc cggcagttcg aaatttgtcg gtttcagcta
     1441 agggcataac ctacttcttg ctaggctcgg cgttactggg cacccgaatc aacaaagaaa
     1501 atacgccgcg gttcgattgt tctttgcgga agacgatttt accaaaatga cgcttaagaa
     1561 tctgttgcgc gaccgccata ctgagacctg cgcgatggcc attaacactg gcaaatgggc
     1621 gaaacacctc gccctcgtcc acttgcaatg aagtttccga ggagctaacg atgttcaact
     1681 ctatatagtt ttccccatcc cgctggcaaa caccggcttc aatattaagc tcaccgcctt
     1741 tagagagaag cgctcgcgaa aaatcgatga cgttggtgag agctctgccg aactgcagat
     1801 ccaggggtac tttcgggaga gactccttga gcttcatccc cgtgcgaatg ccgtgcgccg
     1861 ccatctcctt cgcgcggctc tgaatgactt cggctaatac cgacgccgga tcttcggtcg
     1921 tagcttcgac cgatggtggc aagagatatt cctggatctc gtgaactaac ctgcgaactt
     1981 ggtttattcc acgatagatt gtttctgcgc tgtcgtttgg aatcgcctgg gaaaatcgca
     2041 gaatgtccaa ctcaccccgg atcgcttgaa ggttattacc catctcatgg gttaaaccgc
     2101 gcacaaagtt gcgcatttgg ctggcaccgt gtaaaaagct atccgaaatg acctcttcaa
     2161 gatagaccgt ccaaacgtta ggtttttcga ctccgctggg tgtatataga aaggaaactt
     2221 ctttcaggca aactacttgc gttcggtttt taggttggaa acggtagcag gatgaaaccc
     2281 tggtctcgcc agcttgcaaa ctcttccacg cgcgcaagaa ctccttacga tcgtcaggat
     2341 gaattcgctc caaccagagc gcggcgtcct gcttaaagtc gtgttcgcaa aatcccaata
     2401 gttcccgggc atcatcatta aagaaacaag actggcgatc taaactccag agcgcggcgg
     2461 gagagggtac caattcaatc gcagttcctg catcgataag tccggctcgc aaccggctag
     2521 ctagttccgg ttgcgcgcta ccttcgtgcg cccccgtttg cttcatctta gtgcgtgaca
     2581 gggtgctgat cttgctcatg gacataatga gggaagaaga aatgatctcc tctacccaac
     2641 tttaccgcac aggggatcta tctgaaatgg gcgaaaaagt aaactagtta aaagcgatgt
     2701 ggcgaatatc ctgtttctta gtatcgaggc ctagcctgcg cgcctcaacc gaacacgaat
     2761 gacagacaat catgtctagc aaaagaatcc cgttctcttc gctcgtaaca cgataggcag
     2821 cgcctcgata tatttcgcca caatgctcgc agaccggacc ggttgatttc atctataaga
     2881 cgtacagcaa actgtatgcc tcttcactat aatcggcagg gaaacgtcta aggttcataa
     2941 tagcagagtt tgtattacat ttgggacacc tgccgcgata aacgttccag atgcggcacg
     3001 atgatattac tatgtaggaa attatgcatc ccctaaagga gaccggcaaa actcccgtag
     3061 cccgatcttg taaagaatgg cggccaatac cgcgcataca ttctccaatt gtggcggcat
     3121 cttcaaaact gcaccgacta tcatcaccac tatctgcata ccggagctgt ccattttatc
     3181 cgagagcgaa aagatgtctt cattacaaac accccgatga agctcttgtt tgacctcttc
     3241 gatgtattgg atgccgtccc ggattagctc attgtaaatg gactccaacc cggcactcca
     3301 atcggccggt gtgggcgtca gcggtaaggt agcgtatagc tccttggctt cccttgcctt
     3361 cctggcggca gaaagatagg tcatgatccc cgccactcga gtgggtttag ccgaaaccaa
     3421 caactgggct cccagaatca tgtatagctc ctcaagcccc atgggcagga gttgcatggc
     3481 ctcagccgtt aattcttcga tcgatggctc tttcatgatc gcctcacgtt caatggctgc
     3541 gattctacat ggcgcgggtc ggatagctaa ttcggtacga aaacaacaac agatggaggt
     3601 tttcgagtag cagacgatgg actataagag aaccgagttt gcgctaggga agtctttgag
     3661 cgcattgcgg gtatcgaaaa tgagcggact gtattgcgca atcagcgaat aatcaaacga
     3721 ggaatgatcg gtgagaacgg caacacagtc cattgactgc agcagttctg cgctcagttc
     3781 caccgagttg atcgtttgac cagcgacgga gattgacgac acgtgcggat cagaataact
     3841 gatggaagcg ccttttttac gcagcccttc gagcacatgc agggccggcg attccctgac
     3901 atcgttggta tcgcgcttgt aggttactcc gaggccaaga atctttgcgc ctcgcaaaca
     3961 cttacgccgc tcgtttagcg catcggcgat gcctaacacc gtgaatccgg gcatctggct
     4021 gttgatgtgc ccggccaatt caatcagtcg cggctcgcag ccattcatct tggctcgcca
     4081 ggtcaaatag tagggatcca ccggaataca atggccacct agtcctgggc caggataaaa
     4141 cggcatgaat ccgaaaggtt tggtcttggc cgcttcgatc acctcccaaa catcgatgcc
     4201 gagcgaatgg caggtcaaag ccatttcgtt agccaaagcg atattgacgc tgcggaaggt
     4261 gttctccaac aactttacca tctcagcgca atccgtggag gaaaccggca acgctttctc
     4321 gacgaattgc tcataaagca aaaccgctat ctcggcgcat cgcggagtca cgccaccgac
     4381 tactttgggg atgttacggg tggtaaaatt ttgattgccg ggatcgacac gctcaggaga
     4441 ataagcaaga aaaaaatcac gaccgacatt gagacccgat tcttcgagga tcggcaacac
     4501 cacctcccgg gttgtacccg gatacgtcgt actctcgagt ataatcagtt gtccgggccg
     4561 tatgtgagtc ctaatcgcct caaccgccgc gatcacgtag gacagatcgg gatccttgtt
     4621 ctttcgcaac ggcgtcggca cacaaatatt tagagtatcg actttctccg ccgccgacag
     4681 cgactgagtc gctcgcaacc tgttggccga aacggccgaa agcaaagctt cgccggtgac
     4741 atcgggaata taggaaattc cggagttaat cgaatcgact ctctctcgga cgagatcaat
     4801 gcccgtcact tcgaaccccg cctgcgccat ctcaagagcg agaggcaacc cgacataccc
     4861 aagaccgacg acgcccacct tcgcagatcg atcgcgaatc ttctttttta actcccggcc
     4921 catttgcgag cggtgagtga tagactgctc cttcgatgct atcgcagcct ctacctgata
     4981 attcatagaa cgtctcccct ccgaatacga ttttgaactc agctaacaag atcactgaat
     5041 gcgacaatat cgaggcaatg aaagatttac cgggatcaat gcaaacttcg acagagtcgg
     5101 gccgggcagc atcaaagtat tccagatgat gcccaccgac ctttcactca acaatggccc
     5161 gaattctgtt gctttcacga ggtagtcatc gatcatgcgg tgcatcgtct tcaggcttag
     5221 gcaaccgaac gatggctgac tgaatgccag ctcacgcata atctctggag cgactttgac
     5281 caaatcataa cggactatat aactgagcaa gagatacaga taagttgcgg ggaccgagct
     5341 attgacggct ctcagtagcc actcccatga gaagtccgcc cccgcctgcc ttaacaggta
     5401 aatagtgtcg acgagcgcaa ccaggccgcc gaccctggcg aagtcttgcg cccagtgaga
     5461 ggcgagatag accagctgca gctcgaaact taagcggcgg acctcaatgc cttgaaagcg
     5521 agatggccgg gtttgagccg cgagattctc ccgactgaag atcttggcgc tgccggctcg
     5581 gcgctcggtc gagaacaagc catgatggac ctcgacccac acctgcttgt cttgatgaaa
     5641 aaatggctca aggtgatgat gcttctcata gcggaggccg cccttcgaga gctgccggta
     5701 gccaagttga tgcagtgcag tagtgacagc cgacagtgat tcttttgcaa ccaaaaaatc
     5761 gatgtcgcgc atgagcctca gatgcggctc aggataacat tgttcactga ttgaaatgcc
     5821 cttcagcaag gtcaagggag gaagatctga tctgcaagca tcgccaattt ctcgcagcgc
     5881 ttgaaaatgt tctccgataa caacccgagc agtcagatcc gccgacttca atgaactcca
     5941 gtgagaactc gccacacttt gagggtttcg gcgtacggca cgatacaaca acggtccgag
     6001 gccgtttctt atcgcccaag caaccgtgat ggcttcgaac ttatcgaggg caagatcttc
     6061 gcgatcactc gagatcgcct cgagaagcga gatcagcggc gggtgactgc tggacattgg
     6121 attaagccca tcacccgatg acgctattct tcgggagtct gctaacagcg ttatcggaag
     6181 gattcgggca agtttgcagc gctggattta tctaagatgc ttttcactag ttcacacgtc
     6241 aacccaagcg ctgccgattt gaggtgaaac gatggcaccc tctgggcgat gacggcggca
     6301 ccggcaaggc cgtcttgctc atgagccagc gggttcaatg catttacaaa caaccgcgct
     6361 gccgcctcag ctttggtgat cgcatccacc gtcggcatct tcaattccgg gcaatgctca
     6421 agaaagaaaa gcgcgctcag cagcgccggc ttggcaagca gcgaattcgg caatcgcgcg
     6481 acgggcacat gtagagttga gcatgtgcgc acgctttgcg ccggcaggga gtaaggcagc
     6541 ggtggctctt tttttaagca gagcgcatgt ggataaggct gaacccgcaa agaactcaaa
     6601 tccacgggag ctaactcatc gctcaaataa tcaaatccgt gatgcaaaag cgcccaggtg
     6661 gtagtcgatt ttccggtccc ggaaggcgcc gcgatcagga agccttgacc ggcaaagctc
     6721 aacgccgccg catgtataaa atacagctca cggcggattt tttgtagttc aatggtcagc
     6781 tccttttcca atacaaaaag aagctcacct tcatccgccg cggttatcgg ctcgcggccc
     6841 tgccgagcca cgcaaaattg tgaaccattg cgccgccaga ccgcgtacac gagatcggga
     6901 ttagcgcaat ggtaaatttg catcgccgca tagttggcgc gtatcagctc agccgccgcc
     6961 tgatctcggc actcgatgag gacaacgcag cctactatcc ggaaaactag tttctggtaa
     7021 tcgttcacgg catagtagag cgcccagggc gagagattag cgtcacatcc gctcgccttc
     7081 tttattgatt ttcgttcttg gctggggctt ttgtctcctt tttgctgtcg gaaggatctc
     7141 ccttggagct cctgggcctg ccggaacccg acgcagcaaa tgccggcata gccttcaggc
     7201 tcaagatcac tggcgccaca taagccgctc tcttgacgaa ctctctacgc tttattttcg
     7261 tctcggacat aactaattct cctttcttgt tgtcaacctg tttaatcatc tccaatgagc
     7321 tgtagatctc gcaatctctc gagagtttca atgacttcct tgatagccgt cgactcatcg
     7381 acttcaaagt tttcacaaac ggcattggca atctcagctg ccgactgctg gccatcacaa
     7441 tgttcccaga tgaacgtcgc agtcttgttg aactggtgga tcaagtcgcg gtctctatcc
     7501 aggatgatca attcgccttc aacgagcctg ctctgaacgc ccttacgacg tcgaaggcgc
     7561 aattcattga gtttcgagct ggttactgct tgcggcataa gattcacgag tatcgcgtcg
     7621 ttaaccgaat cgctcacccg ggcatgcacc gacacgatgt ccaggttgtt tgctcttttc
     7681 catccctcaa gaaccggcca aatgcaagct cccaccacct acagatcctg aagaccctga
     7741 tccttgtatg atatagatga acgacttgcg ccaacccggg tgatcagtgc ctgggttgaa
     7801 tcgatcacgc cgagcggcta gccagtttcc aagcccgagt gggttctttg tttgtccctg
     7861 gttgacggat ttggtttgca gtcttgggat cgaccggccg gaatgttggc acagtctcgt
     7921 acagcaaggc taacgcccgc cgtgactggc ctttaagcgc catctgttcc atctggtcaa
     7981 ttttgtcggt gagggactgc agatccgata gccatgcaga ctgcacccga aatatttttt
     8041 caacttcggc ggggatgagc atctcatcca tgcctaccaa ctcttcacgg agtttttccc
     8101 cggggcgaag acccaccacg tgaatcggaa tgtcctcctc cggaacgaat ccagccagac
     8161 gaatgagatg gcgcgccatg tccattaact tgatctgctc gcccatttcc agaacgaaaa
     8221 tctcgccgcc tcgagcaagc gtcgccgctt gcagcaccag caataccgcc tcggggatgg
     8281 tcatgaagta gcggctgatc tccgggtccg tgacggttac aggcccgcct tgttcaattt
     8341 gcttcagaaa caagggcacc acactgccgt tgctacccaa aacattgccg aaacgaacgg
     8401 ccgtaaaaac cgtttggctc tgttcacagc tcttggacag cgcctgtatg cacatctcgg
     8461 caacccgttt ggtggctccc atcacgttgg tgggattgac cgccttatcg gtggaaatct
     8521 gcacgaacct ctcaacctgg tattgaatgg caaaccgcat tagccggtgc gttgcaacca
     8581 cattgttgag gaccgcttcg ttgggatggg tttccatcat cggcacgtgt ttgtaggcgg
     8641 ctgcatgaaa gacaagctgc ggagcatggc ttgcaaaaat ttcttcgatc gctcgtgcat
     8701 ctctgatatc ggcaagaacg gccacgcgct cgacgctctg ctctgagcga tcgagttcgg
     8761 tgttgatatc gtagagagcg ctttctccat tgtctaaaag aaccaactgc gccggttgat
     8821 atcgtgcaat ctgccggctc agctcggaac cgatggagcc gccggcgccg gtaacgagta
     8881 ttcgtttgcc tttcaccaac gcgtgcaccg gttcgagatc caaaccaact ggggctcgat
     8941 ccagtaaatc ctcaatcgct agattgcgca tctgcttgac gccgacattg ccgtttcgca
     9001 attcactgag gcgaggcaag gttctgatcg ggatcttgaa aggttcgagt attttaacta
     9061 cttcacggac catcgccggc ttggccgatg ggatggcgat cacaagctcg tcgggctttt
     9121 cctctgtgat gatccggggc agatcttctc tcctgccaaa gacctgaaca ccatgaattc
     9181 tacggccgat ctttttcgaa tcgtcatcga caaagccgat gggctgataa ccgtaaaacg
     9241 aagaattgtt ccttatatcc cgaactatca tttccccggc gtctccggcg ccatagatca
     9301 gcagccgcct cccttccttt gcttgcccga tgccgtaaac cagacggcgg gtcaaacggc
     9361 tgccacccat aagaaagatg agcagaagag agtcaatgat aaagattgag agaggatagt
     9421 tcttggccgt caatatccaa tgcacagtga tgtagaacaa tccggtgctc acgagcacgc
     9481 cggcgatgac attgcggagg tcccagattc cggtatagcg ccataggcct ttgtagagct
     9541 gaaatggcac gaaggtcagg ccgcggatca acatcaacca gggaatcatc cgcaacatga
     9601 tttccttttc ctgaggtgga atcgcgccgt cgaaccggat ccagaaggcc aggtaattgg
     9661 ctacaacgat caaagccata tggaaagcga caatcggaat ccgtcgatat ttcagggtga
     9721 tttctttcaa catctaattt tgcctcaagt gttgtcttcg agcgtggctg cgcgcccctt
     9781 ggcgattacg atagtggaaa gaatgcagct ggtaaaactg tgacctgctg aggtgaaact
     9841 cagcagtcaa gtaatctgct gaccgcaccc gtagaagcat caacaaagtc cacaactttc
     9901 gttaaccatc gacgggccgc aaaaatggag cggagtaaca ctatacccga ttccgttcag
     9961 gcggttgaaa acttcaactc agagtcggtg ggcgttaaac cacgcaactg tctgtatgat
    10021 gccttgacgc aggtcaaact tggcgcgaaa tccgaagaag cgctcggccc ggctggtatc
    10081 gagacaacgg cgcggttgtc cgttgggctt ggttgagtcc cacacaattt cgccgcgaaa
    10141 gccgacttcc tcggcaatca tttgggccag ttcgctgatc agaatttcct tgccggtccc
    10201 taaatttacg ggctcatcgc catcatagtt ttcgcttgct gagacgatcc cttcagcggc
    10261 atcctcgacg taaagaaatt cacgagtggg cgaaccgtct ccccataacg cgatttgttt
    10321 ttcgttacgc tctttggctt ccatacattt gcggatcagc gccggaatca cgtgagacgt
    10381 attcaagtcg aagttgtcgc cgggaccgta caagttgacc ggaagaaggt agatggcatt
    10441 aaaaccgtat tgcgcgcggt acgattgcgc ttgcacaagc atcatttttt ttgccagacc
    10501 atagggagca ttggtttcct cagggtaacc attccaaaga tcttcttcct taaacggcac
    10561 gggcgcaaat ttcggataag cgcagatcgt ccccacggca acaaattttg caacctggaa
    10621 ctgccgggca tattccataa gttggattcc catgatggca ttgtcgtaaa agaaccggcc
    10681 cggattgtcc cgattcgcgc cgatgccgcc gaccactgca gcaaggtgta tgaccatgcg
    10741 cggctggaag tcctgataca ttcgggtaat tgccgcctga tcgcgcaaat catattcttt
    10801 agagcgcggc ataaaaattt tttcgtaacc acgggatcgc aaacacttga ccaccaccga
    10861 gcccaagaat ccggcgccgc cagtgaccac gataggtagc tgcttcgatt ctataacttc
    10921 tcgtttcaac ggcgcccctt ccttaaagcc gctccggtgc tttgacgtta gacttgttgt
    10981 aactatttag atgagcctct tgtttcgcgc tgaccatatc agcatcgacc ataagccgca
    11041 ccagatcctt gaaggttgtc ttcgcttgcc accgaagctt ttgtttggcc ttggatgcgt
    11101 cacccatgag caaatccact tccgccgggc ggtagtagcg ttcgtcgact tctagatgct
    11161 ttcgccaatc aagcccggca tacgaaaagg cttcttcaac cagttcggaa accgaatgcg
    11221 tctgcccggt ggcgatcaca taatcgtccg gctcctcttg ttggagcatc agccacatag
    11281 cttcgacgta ttccttggcg tatccccaat cacgtttggc ttcgagattg ccgagataca
    11341 ctttctgctc cagcccggct ttgatccgcg ccgcggttcg ggtgacttta cgggtgacaa
    11401 aggtttctcc tcggcgcggc gactcgtgat taaagagaat gccgttgcac gcgaacagac
    11461 cgtagctttc acggtagttc acggttatcc agtatgcgta aacttttgcc gctccatacg
    11521 gactgcgcgg ataaaacgga gtggtttccc gctgcggtac ggcctgcacc ttgccataca
    11581 tttcggaact gctcgcctga tagaatttgc ttttcagccc ggtttcgcgg atggcttcga
    11641 ggattcggat cgtgcccaaa ccgctgacgt cgccggtgta ttcaggtatg tcaaagctga
    11701 ctcgtacatg gctctgggcg gcgaggtgat agatttcatc cggctgaata cgatagagca
    11761 gcttgatgag attggtagaa tcggcaatgt caccatagtg taaaaataac cgcactccgt
    11821 tgatgtgcgg atcttgataa agatgatcaa tccgttcggt gttgaaagtc gaggcacgcc
    11881 ggatgacgcc gtgaacctca taccctttgt ccagcaaaag ctcggcaaga tacgaaccgt
    11941 cctgaccggt aattccggta attagcgctt taggcaagtg agctccgtaa gattactgtt
    12001 tgaataggaa acttgggcta gctttgagtt acgatgtccg accagcatca gccacactcg
    12061 ctgccaatcg agcgtggtta tctttacatc atcgatatgc gggccttgtt ctcaggcgtt
    12121 gaaaatggct tttcgagtat agtttcatac cattcaagat aacgtttaac ttggatctgc
    12181 agatcgaatc gcaaagccgc atccaccttt gcattccttg agagttgatg acgcaaagga
    12241 tcgttgccta gtatccgttc gatcccgagc gccatagact ccgcatcgcc ggcttgggtg
    12301 agaattccag ttgcttcttg cgctgagtaa ccgctcccag agatcgaatc agagctctgt
    12361 agcgacttta tctgctctgg tattccaccg accgctgtag cgacaacggg agtgccgcac
    12421 gccaacgctt caataaccgc ccggggaaat gtatcagctc ttgccgcatg gacataaaca
    12481 tcggcggcgc gatagtaacg ggcaacggct tcgggatttg cctggtgtgg cacaaaatgc
    12541 acctcggctt caccgatttt cacgtcaggg ctattttcgc ctaaagctac aagatgtata
    12601 ttgcctagct ccattcgctt accgaccaga gtgattgcag cttccaaggt attgtaatct
    12661 ttccatacgt tgcgacgtac ttgattggcg gcaaaaagaa gaatcttagc attttgcggc
    12721 aaatgaagcg cgcttcgcgc cgcggatttg tctccagaac gataaatcgc cagatccact
    12781 ccagtaggaa tgactctaga ctcgacgatt gccggagcaa tcatcgattt ttcgactttg
    12841 ctcataagcc actggcttgg cgtagaaata taaattcggc tgccgcggta gatttcccgc
    12901 ttttttcgcc agttgaacac cgttccgtcg cggctgaggg g
//
LOCUS       DASUVS010000453         4122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1951270, whole genome shotgun sequence.
ACCESSION   DASUVS010000453 DASUVS010000000
VERSION     DASUVS010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951270"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(525..998)
                     /locus_tag="VF182_23900"
     CDS             complement(525..998)
                     /locus_tag="VF182_23900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02588 family protein"
                     /protein_id="HEX6440181.1"
                     /translation="MAARSDHTSPRRQGSKLPMLAQARSTSQKQQEKDFIPIWEWIVA
                     AVGLVLVAGVIGFLLYEAFSGERLPPDVKLSVESVVQTRNGFLVRITAVNEGGMTAEG
                     VIIEGELRSGSEVLERSRTTIDYLPPSSEKRGGLFFTRDPRRFDLQVRPLGYEEP"
     gene            complement(1012..1863)
                     /locus_tag="VF182_23905"
     CDS             complement(1012..1863)
                     /locus_tag="VF182_23905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02587 family membrane protein"
                     /protein_id="HEX6440182.1"
                     /translation="MGDQRSQSISPERIAAQSFFIGLARAFGGALIFSLPILMTMEMW
                     WLGVSIDEFRLALLLFLSIPLLIGLSHYMGFEDTFGFKDDALDAFVALAVGFVAGAVT
                     LSLFSVIGSEMSASEFIGKVSIQAVPGSIGAMFAQSELGGKEGKKEEKRRHAGYAGEI
                     FIMAAGALFLAFNVAPTEEMILIAHQMSIWQSIALILVSLLIMHAFVYALEFQGTPSL
                     PPGTPFWSVFLRFTTVGYAVALLMSLYMLWTFGRTEGLALHQTLSILIVLGLPAAVGA
                     AAARLIL"
     gene            2277..3218
                     /gene="coxB"
                     /locus_tag="VF182_23910"
     CDS             2277..3218
                     /gene="coxB"
                     /locus_tag="VF182_23910"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX6440183.1"
                     /translation="MACAGPQSALDPAGRAAERIAEIFWWMTITGTVIWIAVIVLAIW
                     AVGAPVGEAHSQRRSNFLIIGGGAIFPTIVLAALLVYGLAPLPTLLAPAPAGSLKIAV
                     TGEQWWWRVHYQTSDGASVILANEIRLPVGEPVEFQLESPDVIHSFWIPSLAGKIDMI
                     PGRMTRLAVLPTKTGVFRGACAEYCGTSHALMSFDVMVHEKEDFARWLAHQATRGLSP
                     SAPLGIRGEELFLANGCGACHTVRDTPANGVVGPDLTHVGSRLSIGAGTLPNDVEAFI
                     TWIARTEHTKPGVLMPQFGMLPEEELRAMAFYLKGLK"
     gene            3335..>4122
                     /locus_tag="VF182_23915"
     CDS             3335..>4122
                     /locus_tag="VF182_23915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX6440184.1"
                     /translation="MNQPDIEPLAVAPGDEIQRAQARRLEKTWETPSGFRYWSSVNNS
                     DVGLWYTGMTLLFLLFGGVLALLMRIQLAVPNNDFLSAELYNQAFTVHGSVMMFLFAI
                     PIFEAIAVLFLPQMLGARDLPFPRLSAFGFWCFLLGGVFLCGSIFFNAAPRGGWFMYP
                     PLTSTYQSDIGADIWLLGFSFIEIAAIAAAVEMVVGSLKTRPPGMRINLVPLYVWYVV
                     VAAAMIVFAFPPLIAGSMLLEVERAFHWPFFNPAGGGDPLLWQHL"
BASE COUNT          864 a         1173 c         1140 g          945 t
ORIGIN      
        1 ggtataattt gcagctcgga atcttccgga tggacgatcc cttctcctac accgatcgtg
       61 gcgcaacaat ttgtcgcaaa cgccatgcct aacggctctc gcggatagtg gccgagccgt
      121 caggcaggat gcacgtgcca aacgcaaata agtttgcgct gcccattgct gcgcgggaat
      181 tcttgcgatc tggttcaaac aaaggagatt ggccgaccgt cggagttccc gtgcagctcg
      241 tgctcgcacg ccgcctctcg tacgttaggc gcacactgcg cgtaggcttc cagtctcccg
      301 ctcttcgcaa attcgctgca ggcggagaga ggttatttat tgacgacttg atgatcttgt
      361 cttggcggct caggggctgg atcattctgc aaacacgagt gcttcgatgc gctgattcgc
      421 tgcgcgagac agtcgggtat gttgacagcg aagacgctgc tttactcatc cgaatggatt
      481 acaaccggag cgagaccgct tcgacgcgat gccacgtcga gagtttaagg ttcttcatac
      541 cctaaaggac gcacctgaag atcgaagcgc cgagggtctc gagtaaaaaa taggcctccc
      601 cgcttctcag agctgggcgg cagataatcg atggtggtcc ggcttcgctc caaaacctcg
      661 ctgccgcttc tgagctctcc ctcaatgatg actccctccg cagtcatacc gccctcgttg
      721 acagcggtaa tccgcactaa aaaaccgttg cgggtttgaa ccaccgactc gactgagagc
      781 ttcacatcgg gcggcaatcg ctcgccgctg aaagcctcat aaagcaaaaa gccgatcacg
      841 ccggccacca gcaccagacc aacggcggcg acgatccact cccagatcgg aatgaagtcc
      901 ttctcttgct gcttctgaga tgtggatcgg gcctgcgcca gcatcggcaa cttggaacct
      961 tgtcgccttg gggatgtatg atcgcttcgc gctgccatgg gtcaccttcg gctaaagtat
     1021 cagccttgcc gcggcggcgc cgacagccgc cggtaacccg agcacgatca aaatcgataa
     1081 ggtttgatga agggccaaac cttccgtgcg gccgaaggtc catagcatgt agaggctcat
     1141 cagcagcgcg accgcgtaac cgacggtggt aaagcgaaga aagacgctcc aaaagggcgt
     1201 tccgggcggt aacgacggtg ttccttgaaa ctccagcgcg taaacaaacg catgcatgat
     1261 caggagcgaa actaagatca gcgcgatcga ttgccagatg ctcatttgat gcgcgatcaa
     1321 aatcatctcc tcggtggggg caacattgaa agcgagaaat aaagcgcccg cagccatgat
     1381 aaatatttca ccagcatagc ctgcgtgtcg tcttttctct tccttctttc cttctttgcc
     1441 gcctagctcg ctttgcgcga acatcgcccc aatgctgccg ggcaccgctt ggatggaaac
     1501 ctttccaata aattcggagg ccgacatttc agatccgata acggagaaga ggctgagcgt
     1561 cactgcgccg gcgacgaagc ccacggctaa agcgacaaac gcatcaagcg cgtcatcttt
     1621 aaaaccaaag gtgtcctcaa agcccatgta atgagagagg ccaatcagga gcggaatgct
     1681 caagaacagc aacaaggcga gcctgaactc atcgatggaa acccccaacc accacatttc
     1741 catcgtcatc agaatcggta gcgaaaaaat cagtgcgccg ccaaaggcac gggccagtcc
     1801 gatgaagaac gactgggcgg caattctttc aggcgatatt gattggctcc gctggtcacc
     1861 cacgacatcg ctcctgttgt ttgctggcgc gcagagttcg tgtggccgct tggagcaatg
     1921 cttatgccgg tattctgtca taccacagaa tacatggccc gagtggcgaa gctggaacat
     1981 gtcttgctcg atgtatgctt caaaaagcgc ttgactacac gaatcaagcg gggcgtccga
     2041 tcgggttaga cgtgaaactc tcgttctaaa atggtcgtcc caaagctgaa ttttgcattg
     2101 aagacctttc tcgcttttgg cacaaagccg agttcataga tttgcagttt tgtaatggtc
     2161 catcaagcat aatcgaaagg catccacccg tcatcgactt acttaccact cgcatcagct
     2221 gcagtcggcc tcgttgcgtg cctcgaccta tcctcttgct atgtacgctt tcgctcgtgg
     2281 cttgcgccgg tccgcagtcc gcgctcgatc cggcaggacg cgccgcggag cgcattgcgg
     2341 aaatcttctg gtggatgacg atcaccggca cggtaatctg gatcgcggta atcgttttgg
     2401 cgatctgggc cgttggcgcc cccgtcggag aagcacatag tcagcgccgc tccaattttt
     2461 tgatcatcgg cggtggtgcg atttttccta ctatcgtctt ggccgcgcta cttgtctatg
     2521 gtttagcccc cttacccacc ttgttggcgc cggcgccggc gggcagcctg aaaattgccg
     2581 tcacgggcga gcaatggtgg tggcgggtgc actatcaaac atcggacggg gcgtccgtca
     2641 tcttggctaa tgagatccgc ctgccggtcg gagagccggt ggaatttcaa ctggaaagcc
     2701 cggacgtcat tcactccttc tggattccct cgttagctgg caaaatcgac atgattcctg
     2761 gacgcatgac acgcctcgct gtgctgccga ctaaaaccgg cgtattccgc ggtgcctgcg
     2821 ccgagtattg cggcacttcg cacgccttga tgagcttcga cgttatggtc cacgagaagg
     2881 aggacttcgc gcgctggctc gcgcatcagg caacgcgggg gctatcgccc agcgcaccgc
     2941 tggggattcg cggagaagag ctatttctcg ccaacggctg cggcgcttgc cataccgtta
     3001 gagacacgcc agccaacggc gtagtcggac ccgaccttac gcatgtcggc agccggctga
     3061 gcatcggcgc cggcactcta ccgaatgatg tcgaagcttt catcacgtgg atcgcccgca
     3121 cagaacacac gaaaccggga gtgctcatgc cgcaattcgg catgctgccg gaagaagaac
     3181 ttcgagccat ggcgttctac ttgaaaggac tcaagtgaaa aaggtttgtt caaatcgttc
     3241 acgcgttcaa accgtcttgg agttccggcg atttgaaccg agcaaagcgg ttgaacgact
     3301 tgaacgtgat ctgccttgac gtgatctgaa tcttatgaat caaccggaca ttgaacctct
     3361 cgctgtggca ccgggcgacg agatccagcg cgcgcaggcg cggcggctgg agaaaacgtg
     3421 ggaaacaccg tcaggttttc gctactggtc ctcggtgaat aactcggacg tcggcctttg
     3481 gtataccggc atgaccttgc tgtttctgct ttttggcggt gtgctggcgc tcctcatgcg
     3541 cattcaatta gcggttccca ataacgactt tctttcggcc gagctttata accaggcgtt
     3601 cacggtgcac ggctcggtga tgatgtttct tttcgcgata cccatcttcg aggcgatagc
     3661 cgtattgttc ctgccgcaga tgttgggcgc ccgcgacctg ccttttcccc gtctgtcggc
     3721 cttcggtttc tggtgcttcc tgttgggtgg agtatttttg tgcggttcga ttttttttaa
     3781 tgccgcgccg cgcggcggct ggttcatgta cccgccgctg acttcgactt atcaatcgga
     3841 catcggcgcg gatatctggt tattgggatt ttcgttcatc gaaattgccg cgattgccgc
     3901 ggccgtcgag atggtggtgg gctcgctcaa aacccgaccg cccggaatgc gcatcaacct
     3961 cgtcccgctc tatgtctggt acgttgtggt ggcggcagca atgatcgtct ttgcatttcc
     4021 gccgcttatt gccggcagca tgcttttgga agtggagcga gcgtttcatt ggcctttttt
     4081 taatcccgcc ggcggcggcg atccgctgtt gtggcagcat ct
//
LOCUS       DASUVS010000454         3826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1297060, whole genome shotgun sequence.
ACCESSION   DASUVS010000454 DASUVS010000000
VERSION     DASUVS010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297060"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(961..1779)
                     /locus_tag="VF182_23920"
     CDS             complement(961..1779)
                     /locus_tag="VF182_23920"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HEX6440185.1"
                     /translation="MAIVTISHAAFTGGSAIAEQVAAALNYRSVNREVLIEASQRYGI
                     PEAKFQEVLETQGHWWERWLESLRLYRITLQAAMCEVAQTGDLVYHGRAGQELFPGIR
                     HVLKVLIVASMDYRIDQVKEQRGLGGDNARQFLKDLDRVRGRRLRALFNIDWQDPTGY
                     DLILNTSRVTADMAVYLIAEAARRPEYRPTSESLQAFQDLTVAARVQAALITSPKTRN
                     VILNVTSDAGRVTISGILADPDLEKEIVRITEKVPGVKDVTTDIEPPPIEYMHP"
     gene            complement(1992..2372)
                     /locus_tag="VF182_23925"
     CDS             complement(1992..2372)
                     /locus_tag="VF182_23925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6440186.1"
                     /translation="MNVAAGKSKTVLLVGKQPTEIVYLTKHLSIAGYAILLARTGAGA
                     IKTAKEHTPDVVLLDIDLSDMDGIDVVSAIRENPVRAKVPIIAISAFPYMKARCLDKG
                     CNGFIQKPIKALELITQLRTLTKG"
     gene            2972..3112
                     /locus_tag="VF182_23930"
     CDS             2972..3112
                     /locus_tag="VF182_23930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440187.1"
                     /translation="MEKYGFDTEMVYVQGVQMVQVHVAGQLDFSTISSVVYLQASVEG
                     PT"
     gene            3118..3807
                     /locus_tag="VF182_23935"
     CDS             3118..3807
                     /locus_tag="VF182_23935"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440188.1"
                     /translation="MASSIDNQLVKLMVHPPIAKPEDLRGKTLAVTRFGSLTDLLIRP
                     VLKNWGLEPQKDVKLIQIGRMPDIATAIAQTSVDGGVISFPTSVQAEKNEYQNDAGFC
                     GVRFRFAGNHGGRKPALQQKQPRYGVEVSHGLYRGDPELFADRELGIRALRRHGGIND
                     REMLASTYDLFTSRYLKKIPYVNAKGVENSLSLIAESNPRAKNRKPQEFMDTSFMEEL
                     ESTGFISSVWK"
BASE COUNT          896 a          974 c          961 g          995 t
ORIGIN      
        1 acgctcccgg agagctgata cttgcgactg gcagcgttga ccgacaatgc tcccgattca
       61 atttcgctga cgatttgttg tttgaatccc gtttcgaatt tccgtctttg ctttgccatc
      121 gatcgcctcc tcatggcggc ccatcttacc gcctgttgag ccgatcgcac ctcctcaaat
      181 tctcagattt tctctccagt caatggggcg cagtccacta aaggatctca atgatccgca
      241 tcctcgctga gaatatgacg gaccgtctta cgcacaactg ccttcccaca cgatataggt
      301 atttggtgtg actgtataag cttcccaaat ttggccgttc ttttcagtca atcgtacaga
      361 gaaaaagggt tcgggcgctt tgccacggac ttcaactcca atgctcccat gggtaagtct
      421 tatattttcg aattgaaacg tcgatgattc ggtccaactg aaatgcccga tcacgattgt
      481 aggggcggca cccttgcccg cgagcggctc gtcaaggttg tccttttgaa gaaagctggg
      541 gacagttgga tcggtctggt ttttgcgctt agaatcgttt gtttatggag gaagacatgg
      601 caacggactc gatcctaaga gtagcaattg tttatgggtt agtagcctca atgcttctgt
      661 ggcttttctt ttagtgccgc tgcgctctcc gtccttgatt ctactacggc gctattcacg
      721 ataacgcggc tgaagcttgg ctgacccgtt ttcttcttgt cggaagccct gcagctcgat
      781 cgattatctc gcctgtttga aagataatcc tgagcattta gttcgggatt accttgtccc
      841 aactagatac tcacgtcggc aagttaataa agaatggcgg gcacaaatga atctgaccag
      901 gccgccgggt gatcggccgt aattttctag cggcttgatc gattatttag atatgaatcg
      961 ctaaggatgc atgtattcaa tcggtggtgg ttcgatatcg gttgtgacat ctttaacgcc
     1021 gggaactttt tcggtgatcc ggactatttc cttctccaga tcgggatcgg ctagaatccc
     1081 cgaaatggtt acacgtccag catcgctcgt gacattaaga atcacattgc gcgtcttcgg
     1141 cgaagtgatt agcgcggctt gaactcgtgc agcaacggta agatcttgaa acgcctgtag
     1201 ggattctgaa gtggggcgat attcaggcct gcgggcggcc tccgcaatca agtatacagc
     1261 catatccgcc gtgacccgcg aagtattcag aattagatcg tagccagtgg ggtcctgcca
     1321 atcgatattg aaaagcgcac gaagccggcg acccctgacg cgatccaagt cctttaaaaa
     1381 ttgccgcgca ttgtcacccc cgagtcctcg ttgttctttt acctgatcaa tccggtaatc
     1441 cattgaagca acgataagca cctttagaac atgcctgatc cccggaaaca gctcttgccc
     1501 cgctcgaccg tgatagacga gatcgcctgt ttgcgctacc tcacacatcg ccgcctgcaa
     1561 tgtgatccga taaagcctaa gactttctaa ccagcgctcc caccaatggc cctgggtttc
     1621 taaaacctct tgaaactttg cctccggtat tccgtacctt tggctcgctt caattaaaac
     1681 ctcgcggttg acggaccggt agttcagtgc ggctgcgacc tgctcagcaa tggcacttcc
     1741 acctgtgaat gcagcatgag aaatagttac gattgccatg gctctctaac ctccttccca
     1801 cacgctcgtg gaatgcacga aatagccttc gtcatcagat gtctaaatgg tcggtatccc
     1861 actttgtact gcaccaaaac aggcggcatt ctcccactcc tctgagtaag tagtcaaggc
     1921 catattagat acaggttgta gcgtaaaacg gctctggacc gtttgccggc tttggcgaag
     1981 agtacgctcg cttacccctt ggtgagcgtt cgcaattgcg tgatgagctc aagagctttg
     2041 atcggcttct ggataaaacc attacatcct ttatccaggc acctggcttt catatagggg
     2101 aacgcactga tcgcgatgat cggtactttt gctctcactg gattttctcg aattgcactc
     2161 acgacatcaa ttccatccat gtccgacaaa tcgatgtcaa ggagtacgac gtcgggagta
     2221 tgttcttttg cggtctttat agcgccggcg ccagttcgag caagtaaaat agcataaccc
     2281 gctatagata agtgctttgt tagataaact atttcggtcg gttgcttccc gaccaagaga
     2341 actgtttttg attttccggc cgccacgttc attttcgacc gctagacact caaccttggc
     2401 attgagtgac caaagcctta tcgtactatc tccgacatct tacctctgca caatggggcg
     2461 aagcgaactg gcgcagctgt gtaatacgag cggtgacctt cagttgtcga tagggctagg
     2521 cttacgcagg taagttcgag cctccgaagc tgaacgacac atgatcatac agaggacatg
     2581 atgatatcag atcttgacat tgggaaatag ccgcactaag gctgcggagg gatcattcag
     2641 agcaaaccac cgatcgaaga gtgaattggc cccgcgcctg gagcgctgct cggagatcaa
     2701 ataaacagac aatcattttt cttatagcgc tggcatagcg ggaacaatgt ttaaaagaca
     2761 ggtgcaaatg cttcgaccct cgaaaaaaca atggctaaaa caactccagt taccaagttg
     2821 ccgtacgtgc tcactggtat ggctattgtt ctgtccatgt ttttggccgc agcggcgagc
     2881 gaaggtgcaa ctctatcgcg cttgagagtc ggctatccat cccccagcgc cagctttttt
     2941 tccctctttt tgccataaag aagccgggct gttggaaaag tacggattcg acacagagat
     3001 ggtctatgtc caaggagtgc agatggtgca ggttcatgtt gccgggcagc tcgatttttc
     3061 gacgatttct tccgtggttt accttcaggc ttccgttgaa gggccgacct gattcaggtg
     3121 gcaagctcga ttgacaatca gctggtgaag ctcatggtcc atccgccgat cgccaagccg
     3181 gaggatctcc gagggaaaac tctcgcggtg acgcgatttg gatcgcttac cgaccttctg
     3241 attcgtccgg tgctgaagaa ctgggggctc gaaccgcaga aggacgtgaa gctgattcag
     3301 atcggtagaa tgccggatat agcgacggcg atcgctcaga catccgtcga tgggggtgtt
     3361 atctcttttc cgacatccgt ccaagcagaa aaaaatgagt atcaaaacga tgctggattt
     3421 tgcggagtcc ggtttcgatt tgccggcaac cacggtggtc gtaagccggc gttacagcaa
     3481 aagcaaccac gatacggtgt tgaggtttct catggcttat atcgagggga cccggagctt
     3541 ttcgcagacc gagagctggg aatccgcgcg ttgcgtcggc acggcggcat taacgatcgc
     3601 gagatgctgg cttcgactta cgatcttttt acgtcccgat acctcaagaa aattccctac
     3661 gtcaatgcaa agggagttga aaactcgctg agtctgattg ctgagagcaa tccgcgggcg
     3721 aagaaccgca agcctcagga gtttatggac acgagcttta tggaggaact ggagagtacc
     3781 ggctttatca gctcggtttg gaagtgatgc aattttgatc gaatct
//
LOCUS       DASUVS010000455        13309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_424581, whole genome shotgun sequence.
ACCESSION   DASUVS010000455 DASUVS010000000
VERSION     DASUVS010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13309
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424581"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..419
                     /locus_tag="VF182_23940"
     CDS             66..419
                     /locus_tag="VF182_23940"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6440189.1"
                     /translation="MPDRMQVRHIAIRSENPDQLSSFYEKAFGLKVVRRNPRGTKSSI
                     YMSDGFMHIAINAAKDDQPNGIWHFGFLVPSLDPINAITPVHQGETAPGRHAENYIID
                     PQGNRIDVVLDMWPT"
     gene            518..1471
                     /locus_tag="VF182_23945"
     CDS             518..1471
                     /locus_tag="VF182_23945"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440190.1"
                     /translation="MLSALRSWNRWLLISACVGLLWGEAVWGAEKIRLGLSTRNVVLM
                     PFYYAKDKKFFDKYGLEVEIIQIRSNLQLAGLVAGELDFITGIGTAIEGIGKGMPVKA
                     VAVLYRAPLFSLLSALPDPKSLEGKKISVSRIGSESHFNGAVMLEKSGADPKKVTWIQ
                     TGSTALNMLPLERGLVEGAVLSPPFTGVMARKGFKILKRSGDVIDASPFNGLVTTREK
                     IQKHPDRLRNTLRAMLEAIKSIRQDRKAVIAYIMQSFAVDQGVAEEAYDDISGVLLDD
                     MIMAESGLNKYLEMIYGRGETNKPLTVNEILDYSFLKAALK"
     gene            complement(1669..2373)
                     /locus_tag="VF182_23950"
     CDS             complement(1669..2373)
                     /locus_tag="VF182_23950"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX6440191.1"
                     /translation="MRVLLLLNGPRERYVGGADSARQLKWRDYCSPVTQLEIGYLPSI
                     GEGGGGANVYAFGTRDALTLGPLYPDRCATAERDGFDAVIIHCFIDPGLRAARDRVRI
                     PVVGPGEVTLSAGASLSDKIGMVTPSNETVDSHWEQVRGLGLEKRFVGIEAIEGPLLP
                     FPQQDPQTMKEALVAAGRKLIAKGAELICPSGLAYIPIRVSAGEVSRKLGIPVLDPAL
                     LSVRTAEMLVAALGYR"
     gene            complement(2601..3551)
                     /locus_tag="VF182_23955"
     CDS             complement(2601..3551)
                     /locus_tag="VF182_23955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440192.1"
                     /translation="MPNPKLHFYFREPTVDTNMPITDGTIKVEGFDVEIVAREEDADA
                     WDCGFAARVRAYSKGAPHLSIPAFPNRKFRLSYIFVNGSSGIEKPKDLEGKRVGIIQW
                     DNTAGVWARGALQHHYDVDLTRICWRAVQAKQDSVPPGIHIEKVTGDGDSDAILNELL
                     VEAKLDAVIGPNVLPSISRRDSRVRRLFRDYKAEEQDYFRKTGIFPISHVVTLKQEFI
                     ERYPNAPVVLLKAFRLARDEAFNRLEGPDPQVLVVSWIAAAVEEQRALMGEHYWPYNI
                     DDNRTALEAITQFAYEQGLSSEKVEYERFFHPEAAELLGT"
     gene            complement(3911..4537)
                     /locus_tag="VF182_23960"
     CDS             complement(3911..4537)
                     /locus_tag="VF182_23960"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6440193.1"
                     /translation="MVTRFALLKELSTIITDDVIVLTSIGNNSGFWGQLKDREANLFH
                     ITMGMCSPTALGLSLALPNRKIVAIDSDGNMILNLGVLGTIANEAPKNLTIVLMDNQN
                     YLGSHKHAPGMPTATAGKMNLEAVAKACGIDSACTVQDVNEFRSGFQAALSSIGPHFI
                     RARVEPLDRDRPERSKRVPDPRENKYQFAGYIQRTEGVEILGSGLGGG"
     gene            complement(4531..5028)
                     /locus_tag="VF182_23965"
     CDS             complement(4531..5028)
                     /locus_tag="VF182_23965"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6440194.1"
                     /translation="MRPEKAQLVLKGLKEAGVDFATGVPDAQFIEVYRMLAEDPEVHY
                     VGAANEAEATGIGFGAWFGGKKPALILATSGLLVATYHLARINLLHEVPVLVVIPYRG
                     DIGDPRWLGLYKKTTEPGLEAMDIPYRIVSRPEDVVRTIKECNESARAWLKPVAVLLN
                     QEALW"
     gene            complement(5040..6050)
                     /locus_tag="VF182_23970"
     CDS             complement(5040..6050)
                     /locus_tag="VF182_23970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440195.1"
                     /translation="MIFHAVAPAFSQTPFYRGKTITMIRGGAPGGVGDMRTRAVAKYL
                     TKHVPGNPTIIVEFMPGGGGQKAANHLYRGAPANGLVIGSAPGGMVFSAVLGEVGVRY
                     DLDKFIYLGSPNSASHYLFFTDRKLGLGSIDKLRAHSGLRIGAQTVGHPIYYTGRLFA
                     FMLGLKEPKFVVGYSSPELDVALLNGELDAVSGVASSLLKQNPEFLEKGLTDFHVVME
                     IPQGNQHPQFAHVPKIDSFAKTDKDRKLLTLHRAFRLAGSPFVLPPETPKEQADILRN
                     AMRRVYLDPEFLAEYEKLTGEEASPLLPDAHQTAMRELPRDADVIEFYKSLGGTKPLP
                     TR"
     gene            6758..7957
                     /locus_tag="VF182_23975"
     CDS             6758..7957
                     /locus_tag="VF182_23975"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6440196.1"
                     /translation="MEIRYKLISADSHVAFDRDAFTSRMSAAKWGNLIPQVVEVRHDG
                     YPVDRWSVYGKPAGDTICNCPALMGDPFPTYPQRWEEVPRTAFDPLERLKALDRDRVD
                     AEVLFPNPPGGTFFEFGDAEFELTVVQAYNDVLSDWARASDRYVPLAILPYLSEPTTI
                     AREITRAAAAGHRGINCLGETPKGLPHLCDPRWYPVWDVCQELELPIHIHGSAGLRAG
                     ASVRKWEGYTPRQAHSAMTATSAVTPAQVVPQLIFSGITERFPKLKFVFAEAGMGGLN
                     YVIAACDHEWECRHLWTEGISTRPSEVVRRQMYVNFWFEMEGIKLRHDIGIDNIMWES
                     DFPHVASYYPRSWDEVERVLEGVPGEDRRKILYENATRVYRIEAKISEESAGSLHHLV
                     SEAGNAA"
     gene            7982..9124
                     /locus_tag="VF182_23980"
     CDS             7982..9124
                     /locus_tag="VF182_23980"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6440197.1"
                     /translation="MKDFGVISVDDHIQEPPDLWSKRISGPRWQNQIPHLEHTPEGKE
                     RWIADGNVLLDGYSARAGALLPDRNRGLERWCDVPLAAYVPSERLKAMDAAGIDYSVL
                     YPTVAGLAGEAFGRFDDSDLELACVQAYNDWILEEWAAASERFIPQCIVPIWPIEATV
                     REIRRAVANGHRGVIFPSLPMHLRPVPHISGSEYDPLWAACEELNVPICLHAGASPEL
                     QYTPPSGLPPAIAAALDAVTKPVSSVFVISLLSFSRVLLRHPRLRLVLAESALSWGML
                     YMEWADHQFDHDGLAREGYDLKPSEMFRRGCYFTSWFDEIAPFANYVGADRILWCAKL
                     PLATSTWPHTRESIERCFRGASPEIQQQVLFENAARLYGLAKALPL"
     gene            complement(9365..10327)
                     /locus_tag="VF182_23985"
     CDS             complement(9365..10327)
                     /locus_tag="VF182_23985"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX6440198.1"
                     /translation="MLTASEIGGVLGMMPAFATPDAVDIRATQTIAVDHLTEGVDKII
                     KDGVNNIATTGTYGECYNLLFDEFKTLAIATIEAAKKRVPVFIGCTSPNPREVVQKMN
                     FVSETGADGVLLGVPYYEQLPVSDAIKFFRDIAELFPKLSIVIYHNPENHKFTMPVPA
                     FKELVKNPNIIGMKDSHRTTQAFMNLQKIVKGKISVFVNQTQLYPYFKLGAAGCWSTE
                     VWMGPWPVLYLFDVVKRADDERAMEVIDDIIGDGSGGRPVPGAGNKRPAEFADYCKVG
                     PTRVPFLHFPETELAKAMRRAEHWMKLNVKYKPLVESLKSRSAA"
     gene            10860..13094
                     /locus_tag="VF182_23990"
     CDS             10860..13094
                     /locus_tag="VF182_23990"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6440199.1"
                     /translation="MKVEREFSLIGTSVQRVDGADKVTGKARYAGDLVVPGMIEGKFL
                     RSPYAHARILSIDTRPAEALPGVIAVLTSKDFTDVSPYMGRGKNKDQPIIAVDRAIFA
                     GQPVAAVAAVDRATAEEALGRIAVEYEELPAMIEVEDALAAGAPRIHEFAEKNICFQT
                     ELVKGDVESGFAEADEIFEDTFEFPMIYHYSMEPHIAIAEVDSDGINIWTSTGHPFGV
                     RQEIAEIYHYPLSKIRVHVNFVGGAYGSKSGGKIEPLVVALARKAQRPVRVALNVAEA
                     MATCRRHAIQCKLKTGVKKDGTLVAKQAEIYLNTGAYAETGPIVTGRTLTRILGPYRY
                     PNLKINSYCVYTNTVSAASFRSIGGPQTAWATESQMDIIAQKLGHDPVELRLKNLVKK
                     GEEIRPKLKPLDADLAKGLKLVTDRLGWDGALSKTGRGRGVGFGTTDPGAPLASTSTV
                     HVLADGSVVLLCGTVELGQGAKTVMSQIVAEELSVPLERVTIRPIDTAFTPFDRSTGS
                     SRSTTVMGKAVELAGSDARRQILELAQEHFESPEEAITLKDGEAIAGGKKVSYGELIH
                     KHFAMQGGELVGRGYAHSGMAPTPANPLFWEIGIGAVELDVDQETGEVRVHKYVTAAD
                     AGKALHPLHCEGQDEGSAMMGFGHTFYEAYQFDSGQIINAGMVDYKVPSFDDVPDQFD
                     SILIEDANGPGPYGAKGLGEGGIIPVAPVIANAIAWCSGARIKTLPLTPEKVWRAIKA
                     TEEK"
     gene            13103..>13309
                     /locus_tag="VF182_23995"
     CDS             13103..>13309
                     /locus_tag="VF182_23995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440200.1"
                     /translation="MAGSNEFEKKSSPNVRGNPVIDSSTQRDKLNPNNSRHEFFACAR
                     CQHPEGTPSAAPRQSMRYELSDNTH"
BASE COUNT         3050 a         3613 c         3582 g         3064 t
ORIGIN      
        1 attgacagaa gagtgggttt gttgtctact ccgctcgaaa acgaccaatt cgaaaggaga
       61 attttatgcc ggatagaatg caagtccgac acatcgctat ccgctcggag aaccccgacc
      121 agctcagctc gttttacgag aaagccttcg gcttgaaggt tgtcaggaga aacccgcgcg
      181 gcaccaagag cagcatttac atgagcgacg gcttcatgca tatcgccatt aacgcagcca
      241 aagacgatca gccgaacggt atctggcact ttggctttct ggtaccatcc ctcgatccca
      301 tcaacgccat cactccggtt catcaagggg agacggcgcc aggccggcat gctgaaaact
      361 atattatcga tccccagggc aatcggattg acgtcgttct cgatatgtgg ccgacttgaa
      421 agacgcggtc gataactgcg ctttgccggt gtaagtcttc gccggcaaag agcgtatatc
      481 cgccgcgctt cttccgtaaa cggttggaga aggatttatg ctgtcagcgc tgcgaagttg
      541 gaataggtgg ctgcttattt cggcatgcgt cggattgctt tggggcgagg cggtgtgggg
      601 tgctgaaaag atccggcttg gactatcgac gagaaacgtc gtcttgatgc ctttctacta
      661 cgccaaagac aagaagtttt tcgacaagta cggcctggag gtcgaaatca ttcaaatcag
      721 gagcaatctt cagttggccg ggctggtagc gggtgagctg gatttcatca ccggcatcgg
      781 cacggccatc gaagggatcg gcaagggaat gccggtcaaa gccgtcgcgg tgctttaccg
      841 ggccccgctc ttctctctgc tcagcgcgct accggacccc aaatcgcttg aaggcaaaaa
      901 aatttcggtt tcgagaatcg gttctgaaag ccatttcaac ggagccgtta tgctcgaaaa
      961 aagcggcgcc gatcccaaga aggttacctg gatccaaacg ggaagcactg ccttgaacat
     1021 gcttccgctc gagcgaggtc tggtcgaagg cgctgttctc agcccgccgt tcaccggcgt
     1081 gatggcgcgt aagggtttta agattctcaa gcgaagcggg gatgtgatcg acgcctctcc
     1141 ctttaatggt ctcgtcacga ccagagaaaa aattcaaaag catcccgatc gcctgagaaa
     1201 cacgctcaga gccatgctcg aagcgatcaa gtcgattcgc caggaccgca aggccgtgat
     1261 tgcttatatc atgcagagtt tcgccgtcga tcagggtgtc gcagaagagg cctacgatga
     1321 tatcagcggc gtattgctgg acgatatgat tatggccgag agtggtttga acaagtacct
     1381 ggaaatgatt tacggtcgcg gcgaaacaaa caaacccttg acggtaaatg agatcctcga
     1441 ttattcattt ttgaaggcgg cgctgaagta gttcacaggg cggattgacc catcaagtgc
     1501 acagagcggc agagccgcaa agttcaccgg gttcaaagta tctttgattg aaaacttttt
     1561 actctgcggc ctcggcgcct cggcggtgaa atctctgaat cgtttcactg cagttctgac
     1621 ttcgcagtat tccttcgtgc tcttcgcggg tcttcgtggg accgattctc aacgataccc
     1681 gagagctgca accagcatct ccgcggttct caccgaaagg agcgccggat cgagaaccgg
     1741 aatccccagc ttgcgcgaaa cttcaccggc ggaaacccga atcggaatat aagcgagccc
     1801 tgaagggcag atcagttcgg cgccttttgc gatcagcttt cggccggcgg caaccagagc
     1861 ctccttcatc gtttgcgggt cttgttgcgg aaatggcagg agaggacctt cgatcgcttc
     1921 gattccgaca aatcgcttct cgaggccaag acccctcact tgctcccagt gcgaatccac
     1981 ggtctcattg ctcggtgtca ccatgccgat cttatcgctt agactcgcgc cggcgctgag
     2041 cgttacctct cccggcccga caaccgggat gcgcacgcgg tcgcgcgccg cgcgcaagcc
     2101 cggatctatg aagcagtgaa tgataaccgc atcgaatcca tcccgttccg ccgttgcgca
     2161 acgatccgga tataacggtc cgagagtcag cgcgtccctc gttccaaatg cgtacacgtt
     2221 ggcgccgccc ccgccttctc cgatgctagg caaatagccg atctccagtt gggtgacggg
     2281 cgaacagtag tcgcgccatt tgagttggcg cgcgctatcg gcgccgccga cgtagcgctc
     2341 gcgaggaccg ttaagcaaga gcagaactcg cattcatccc tccgggattc tctgttatga
     2401 taaatgcttt tgtgttgacg tttctacaga agatatcgtt tgcggcaaat cgggtgatac
     2461 ggtgatttcg aaaaaccctg tatcatcgcc cggacgaggt gaccgcgtta catggagaga
     2521 gataccggac ggcgatggca gcgcatccag ttttggatct tcaacgttac ctttcagttg
     2581 attcatagcg ttgttccctc ttacgttccc aagagctcgg ctgcttccgg atgaaaaaac
     2641 cgctcatact cgaccttttc tgaagagagc ccctgctcgt aggcgaactg tgtaatggct
     2701 tctagagcgg tccgattgtc atcaatgtta tagggccagt agtgttcgcc catcaatgcc
     2761 cgctgctctt caacagcggc cgctatccac gaaacaacca aaacctgcgg atcaggtccc
     2821 tcaagccgat tgaaggcctc gtcccgtgcc aagcggaatg ccttgagcag taccaccggc
     2881 gcattcgggt agcgctcgat gaactcttgc tttagcgtta caacgtgact aatggggaaa
     2941 atgccagttt ttctgaagta gtcttgttcc tcggctttgt agtcgcggaa aaggcggcgc
     3001 acgcgcgaat ctcttcgtga aattgacggt agtacgttgg gcccgatgac cgcgtcgagt
     3061 ttcgcttcga ctagcagctc attcaaaatc gcgtccgaat cgccatctcc agtcactttt
     3121 tcgatgtgaa taccgggagg gacgctgtct tgtttcgcct gtaccgcccg ccagcagata
     3181 cgcgtcagat cgacatcata gtgatgctgc agtgcgcccc gtgcccacac gccggcggtg
     3241 ttatcccatt gtatgatgcc tacgcgtttg ccttctaaat ccttcggctt ctctatgcca
     3301 gaggagccgt tgacgaagat gtatgaaaga cggaacttgc ggttggggaa cgccggaatt
     3361 gagaggtggg gggccccttt tgaatacgcg cgcacacgcg ctgcgaagcc gcagtcccag
     3421 gcgtcggcgt cttcttccct ggcaacgatc tcgacatcga aaccttcgac cttgatcgta
     3481 ccatctgtga tgggcatgtt ggtgtcgaca gtgggctctc ggaaataaaa gtgaagttta
     3541 ggattgggca tttgttgctc tctttgaatt agacttttgc acctttacgg tgccttttcc
     3601 tctttcatgt aaaacccacc ctttttcatc ccaggccaat ggtccaccga aaggagtatc
     3661 gtgtcatgat tgacgttgct ctcgtggtgg tgcctagtca cttgcgaaaa catacagtct
     3721 gttcgcatac ggaaatcgct acatcgcaga tcaccaaaat cggaaaatgt ttgaccgctt
     3781 cacagtattc atgctgtatg gtgcggttcc tcagcgatag ctatagcagg gaatccgaga
     3841 tctaaggctc agccgtcgtg ctatggagcc caagaagaat gaaaaaatga gttactgttt
     3901 ttcaattgtc tcaaccgcct cccaagccac ttccaagtat ctcaactcct tcagtcctct
     3961 gaatataacc ggcgaattga tacttatttt cgcgggggtc gggcacgcgt ttagacctct
     4021 ccgggcggtc cctatctaac ggttcgaccc gggcgcggat aaagtgcggc ccaatgctag
     4081 acagagcagc ttgaaacccg ctacgaaatt catttacatc ttggaccgtg caggcgctgt
     4141 cgattccaca agccttggca acagcttcca gattcatttt acccgcagtt gcagttggca
     4201 taccgggtgc gtgtttatga ctgccgagat aattttggtt atccatgagc acgatagtta
     4261 aattcttcgg cgcttcattg gcgatggtac cgagcacgcc gaggttcaaa atcatgttgc
     4321 cgtctgaatc aatagcgact atcttgcgat tgggaagcgc cagtgaaagt cccaatgccg
     4381 tggggctaca catgcccata gtgatatgaa atagattggc ttccctatct ttcagctggc
     4441 cccaaaatcc actgttatta ccgatactag tcagcacgat cacatcatcc gtgattatcg
     4501 tcgacagttc cttgagcaac gcgaatctgg ttaccataac gcctcctggt tcaacaacac
     4561 ggcgaccggc tttaaccacg cgcgtgcaga ttcattacat tccttgatcg tccttacgac
     4621 atcctctggt cggcttacga tgcggtatgg aatatccatc gcctcgagac ccggctcggt
     4681 ggtttttttg tagagaccca gccagcgtgg atcaccgatg tcgccgcgat acgggatcac
     4741 caccaaaacc gggacctcgt gcagcaaatt tattcgtgcc aaatgatacg tcgctaccaa
     4801 taagccggag gtcgctagaa ttaacgccgg cttcttaccc ccgaaccagg cgccgaaacc
     4861 gattccggtc gcctcagctt cattggcggc gcccacatag tgaacttcgg gatcttcggc
     4921 aagcattctg taaacttcaa tgaactgcgc atcgggaaca ccggtggcga agtccacacc
     4981 cgcttccttg agccctttaa ggactagttg cgctttttcc ggcctcatac tttcctccgt
     5041 taacgtgtcg gcagcggttt tgttccacct aatgatttgt agaattcaat gacgtcggca
     5101 tcacgaggca gctcccgcat tgccgtctga tgcgcatcgg gcaagagcgg tgatgcttct
     5161 tcgcccgtca atttctcgta ttcggcaaga aactctgggt ctaagtagac tcggcgcatg
     5221 gcattgcgca gaatatccgc ttgctcttta ggggtttccg ggggcaacac gaaaggcgat
     5281 cccgcaagcc tgaaagcacg atgaagggtc agcagtttcc ggtctttgtc tgtcttggcg
     5341 aaactatcga ttttcggtac gtgggcaaac tgagggtgct gattcccctg cggaatctcc
     5401 atcacgacat gaaaatccgt taagcccttc tctagaaact ccgggttttg cttcaaaagg
     5461 ctgcttgcga cgcccgacac agcatccaac tcgccgttca gtaaggccac atcaagctcg
     5521 ggcgacgaat aaccgacgac aaatttgggc tctttcaagc caagcataaa agcaaacaga
     5581 cgtcccgtgt aatagatcgg atgaccaaca gtctgagccc ctattctaag ccccgaatgt
     5641 gcccgcagct tgtcgatgct accaagtccc aatttcctat cggtaaaaaa caggtaatgt
     5701 gatgcactgt tgggcgagcc gagatatata aacttgtcta aatcgtatcg aacacctact
     5761 tccccaagta cggcggagaa caccatcccg ccaggggcgc tgccgatcac aagtccgttt
     5821 gcgggggcac ctcgataaag atggttggca gctttttgac caccgccgcc cggcataaac
     5881 tcgacgatga tcgttggatt accgggaaca tgcttcgtta gatacttcgc cactgcccgg
     5941 gtgcgcatat cgcccactcc gccgggcgcc ccgccgcgga tcatggtgat ggtctttccc
     6001 cggtaaaacg gagtctggga aaacgccggt gcaacagcat gaaaaatcaa ggaagccgtt
     6061 aaaacggtga ttttagctcg cgctaacatt cctatcacgc gtaacctcct acatcgcgcg
     6121 aaatcgcgcg gcaatgcttt ggacgcgccc catcaatcag gaaacagcat tcgtcaccgc
     6181 tgccatcaac tgcactgccg cgccgagtag ctggagcgcg taaattgcgc cgtctatgcc
     6241 atcatcgact gatctcgact tacgcgtagt tatgtaatcc gatcgatcgt gaggtgttta
     6301 ccgatcattc cgatggcaac cacaatcttg tcagagttga gcataaagct gctctcagtt
     6361 cctccggagc aaacagcaca ggccgagagc gtctcgtcta tcgatgggta cgcaacgtcg
     6421 catggattcc gatgagggcc cggaaactcc tgaatcatgc cggagttgtg cgatcgcaga
     6481 tatcacacgt gaattcatca gcaccacccg cgcgatcaac cccccgggcg cgaggacttc
     6541 aagtggtggc ccccgtcatc actgatgagc cgcgaccgct gcgacgactg taagcgaagg
     6601 gctatttaca acattgtcga tcgggagttt ttccttatcg ctggagaatt ctgttgtcaa
     6661 ggcacttatc gtcaccaatg ccgtagttta tttgtagtga ccgctcgatt aagttatatc
     6721 tgccgtacga catttgagag agagagggag agcggatatg gaaatcagat ataaattaat
     6781 cagtgcagac tctcacgtcg ccttcgaccg ggacgcgttt acctcacgca tgtcggccgc
     6841 gaaatggggg aatcttatcc cccaagtcgt tgaagtgaga catgacggct atccagtcga
     6901 tcgttggagc gtctacggca aaccggccgg cgacaccatc tgtaactgcc ccgctcttat
     6961 gggtgatccg ttccccacct atcctcagcg gtgggaagaa gttccgagga ccgcgtttga
     7021 tccattggag cgcttgaagg ctctggatcg cgatcgcgtg gacgcggaag tactgtttcc
     7081 caatcctcca ggaggtacct ttttcgaatt cggagacgcc gagttcgaac tgaccgtggt
     7141 acaagcgtac aatgatgtgt tgagcgattg ggcacgagcc agtgaccgat acgttccact
     7201 tgccattctt ccctatttaa gcgagccaac aacgattgcc cgagagatca cgcgggcagc
     7261 ggcagctgga caccgaggaa tcaattgcct aggagaaacg cctaaggggc ttccacatct
     7321 ttgcgatcct cgttggtatc cagtctggga tgtatgtcaa gaactcgagc ttcccattca
     7381 catccacggc tcagcgggat tgcgtgccgg cgcctcggta cggaagtggg agggatatac
     7441 gccccggcag gctcactcgg cgatgaccgc aacatcggcc gtcacaccag cccaggttgt
     7501 tccccagcta attttttctg gcatcaccga acgctttccc aaactgaagt tcgtgtttgc
     7561 cgaagccgga atgggagggc tcaattatgt aatcgctgcg tgcgatcacg agtgggagtg
     7621 ccgtcacctg tggaccgaag gcatcagtac gcgccctagc gaggttgtgc gtcgccagat
     7681 gtacgtgaac ttttggttcg agatggaagg cattaaactt aggcacgaca tcggcatcga
     7741 taacatcatg tgggagtcgg actttccaca cgtggcttct tattatccgc gatcatggga
     7801 cgaagtggag cgcgtcctcg aaggagtgcc gggagaagac cgccgtaaga tcctttacga
     7861 aaatgcaact cgcgtttatc gtatcgaagc caagatctcg gaagaatctg cgggaagctt
     7921 gcatcatctc gtttctgaag ccggaaatgc tgcttgactc acaaccgaga ggaacttcat
     7981 cgtgaaagac ttcggcgtaa ttagcgtcga cgatcatata caggagccac cggacctttg
     8041 gagtaagcgg atttccgggc ctcgctggca aaaccagatc ccccacctcg aacatactcc
     8101 cgagggtaag gagcgctgga ttgccgacgg taacgttttg ttggatggct atagcgcacg
     8161 agccggagct ttactcccgg accgaaaccg cgggcttgag cgttggtgcg atgtcccatt
     8221 ggcagcttac gtgccgtcgg agcgtcttaa ggctatggac gcggccggca tagattactc
     8281 cgttctctac cctactgtag caggtttagc gggcgaggcc ttcgggcggt tcgacgacag
     8341 cgatctcgag cttgcgtgcg tccaagcgta taacgattgg attttagagg aatgggctgc
     8401 tgctagcgag cgttttattc cacaatgcat tgttccaatc tggccgattg aagcaacagt
     8461 gcgtgagata cggcgagccg tagccaacgg ccatcggggt gtgatttttc cttcattgcc
     8521 catgcatcta cgtccggtgc cgcatattag cgggtccgaa tacgatcccc tttgggcagc
     8581 ctgtgaagaa ctcaacgttc ccatttgcct ccacgctgga gcttcgcccg agttgcagta
     8641 cacccctcca tccgggctgc cacccgcgat cgcggccgcc ttagacgcgg tgacgaagcc
     8701 ggtctctagt gtgttcgtta tctcgctgct ctcattctca cgggtattac tacgtcatcc
     8761 gagattgcgc ctggtgctag cggaaagcgc gctcagttgg ggtatgctct acatggaatg
     8821 ggccgatcac cagttcgatc acgacggtct cgctcgtgaa ggttacgact tgaagccctc
     8881 agagatgttc cgccgtgggt gctattttac ttcgtggttc gatgaaatcg cgccatttgc
     8941 gaattatgtc ggagctgatc gtattctgtg gtgtgccaaa ctaccgttag ccacctctac
     9001 ttggccgcat acaagggaaa gcattgagcg gtgtttccga ggcgcctccc cagagatcca
     9061 gcagcaggtg ctatttgaaa acgctgcccg gctttacggg ctcgctaagg cacttccgct
     9121 gtaaattcag caccgcgtga ccgacggccc tgtggtacgg ttggcgctta cgctcgaacg
     9181 caagaaccac tttttttatt gtacagcttc aaccgctaga aaacgtggat gactttgcac
     9241 attcgcctag gcttaacgtc agacctcccg aagacatagg agacccatta cttatagcct
     9301 tcatcgtcat acgcgtctga gataaggctt taacgagggg agtggcccac cacatcgcaa
     9361 ccgctcaagc tgcagaacgg gattttagcg actccacaag cggcttatat ttgacattga
     9421 gcttcatcca atgctctgct cgacgcatgg cttttgctag ttcggtttcc ggaaaatgaa
     9481 ggaacggaac ccgagtaggg ccaactttgc aatagtcagc gaactctgcc ggtcgcttgt
     9541 tgcctgcgcc gggaactggc cgtccaccac taccatcacc tattatgtcg tcaattacct
     9601 ccatcgctct ctcatcatcg gcgcgtttga cgacatcaaa caagtataaa accggccagg
     9661 gacccatcca gacctcagtc gaccaacatc cagcagcacc cagcttgaag taaggataca
     9721 attgagtttg atttacgaaa acgctgattt tccctttgac tatcttctgc agattcatga
     9781 aagcctgtgt ggttctgtgg ctgtccttca tgccaatgat gttgggattt tttactagtt
     9841 ctttgaaagc tggtaccggc atcgtaaatt tgtgattctc cgggttatga taaattacga
     9901 tgctgagctt tggaaatagc tcggcgatgt cgcggaaaaa tttaatcgca tcagacaccg
     9961 gtaactgttc ataatagggt acaccgagca aaacgccgtc cgctccggtt tcactgacaa
    10021 agttcatttt ctgcacgact tcgcggggat tgggactcgt acagccgatg aagacaggca
    10081 ctcttttttt tgccgcttca attgtcgcga tggccagggt tttaaattcg tcaaatagga
    10141 ggttgtagca ttcaccgtat gttcctgtcg tggcgatgtt gttgacgcca tccttgatga
    10201 tcttatcgac gccttctgtg agatggtcga cagcgatcgt ttgggttgcg cggatatcga
    10261 ccgcgtccgg agtggcgaag gcaggcatca tcccaaggac cccgccgatt tcactggcag
    10321 taagcattga gcacctccat atctttttct ttgacgatca atctaacacg agtggtcagc
    10381 gttcttcgat ctttagcagg caccgttgcg ctgtgtcaat ccaacttatt gggtcaccat
    10441 ctttgcacga agacggtttg tttcaatggg agtgaagact taagttccag gttagtgatg
    10501 tctttcagtg ctaaatcata tgagaatttg cagtgtagct gggcagagga gtgtttcgcg
    10561 ggcatcaatg aactttatca agccgtcctc cgattgtgac gggatgctta agatccgcgc
    10621 agaagatgaa agctgaactg taaaaatgaa gcgaaatcag agccggcgct ttttaaggca
    10681 ctgccgcctt tggcgcaatc catgatcgtt taaccggtcc aagagcttga cagggattta
    10741 tcgccaatat acgggaggat gtgtcgacag ctcgtctgtt ggcaaagcta ctgttccatc
    10801 gtccctcaaa ccgccgcgaa gacaattctg atggattctc ggcaaaagga agtttcaact
    10861 tgaaggttga gcgcgagttt tcgttaatcg gcacatcggt gcagcgcgtt gacggcgccg
    10921 acaaggttac cggcaaggcc aggtacgcgg gcgatctcgt tgttcccgga atgatcgaag
    10981 gaaaattcct gcgcagcccg tacgcgcacg cgcgcatcct ctccatcgat acacggccgg
    11041 cagaagcgtt gcccggcgtg attgccgtgc tcaccagcaa agacttcacg gatgtgagcc
    11101 cgtatatggg ccggggaaaa aacaaagacc agccgatcat cgcggtggat cgagcgatct
    11161 ttgccggaca acctgtggca gccgtcgctg ccgtggatcg cgcgaccgca gaagaggcgt
    11221 taggcaggat tgcggtcgaa tacgaggaac tgccggcgat gatcgaagtg gaggatgcgc
    11281 tagcagccgg cgcgccgcga attcacgaat ttgccgagaa aaatatctgc ttccagaccg
    11341 agcttgtcaa aggcgatgtc gaaagcggct ttgccgaagc tgacgagatc tttgaggata
    11401 ctttcgagtt cccgatgatc tatcactact cgatggagcc gcatatcgcg atcgccgaag
    11461 tcgatagcga cgggatcaat atttggacgt ccaccggtca cccgttcggc gtgcgccagg
    11521 agatcgcgga aatctatcat tacccattat ctaagatccg cgtccatgtg aattttgtcg
    11581 gcggcgccta tggcagcaaa tcgggaggaa agatcgagcc gctggtggtt gcgctggcgc
    11641 gcaaagcgca acggccggtt agggtggcgc tcaatgtcgc ggaagcgatg gccacgtgcc
    11701 gccgtcacgc gatccaatgc aagctcaaga ctggcgtgaa aaaagacggc accctggttg
    11761 ccaagcaggc cgagatctat ctcaataccg gtgcgtatgc tgaaaccggt ccgatcgtga
    11821 ccggtcgaac cttgacccgc atacttgggc cgtaccgtta tcccaatctg aaaatcaact
    11881 cctactgtgt ctataccaac accgtgtcgg ccgcttcctt ccgttccatc ggcgggccgc
    11941 agactgcctg ggcgaccgag tcgcagatgg acatcatcgc gcaaaagctc gggcacgatc
    12001 cggtagagct gcggctaaaa aatctcgtca aaaaaggcga agagattaga cccaaactca
    12061 agccgctgga tgccgatctc gccaaagggc ttaaactggt caccgacaga ttgggctggg
    12121 atggagcgct cagcaaaacc ggccggggtc gaggcgtggg attcggcacc accgatcccg
    12181 gagcgccgct agcctcgaca tcaacggtgc atgtgctcgc cgatggcagc gtggttctct
    12241 tatgtggaac agtggagctc gggcagggtg ccaaaacggt catgagtcag atcgtcgctg
    12301 aagaattatc agtgccgctc gagcgggtca cgatccgccc catcgacacc gctttcactc
    12361 cgtttgaccg ctccaccgga tcgagccgtt cgaccaccgt gatgggcaag gcggttgaat
    12421 tagccggaag cgatgcgcgc cgacaaatcc tcgagctggc ccaggaacat ttcgaatcgc
    12481 cggaggaagc gatcactctg aaagatggcg aagccatcgc cggaggaaaa aaagtttcct
    12541 acggcgaatt gattcacaag cattttgcca tgcagggagg cgagctggtc ggccgcggtt
    12601 acgcccatag cggcatggcg cccactccgg caaatccgct gttttgggaa atcggcatcg
    12661 gcgcggtgga gctcgatgtc gatcaggaga ccggagaagt gcgcgtgcat aaatacgtta
    12721 ccgcggcgga cgccggcaaa gcgctgcatc cgctacactg cgagggccag gacgagggtt
    12781 cggcgatgat gggatttgga cacacgttct atgaggccta tcaattcgac agcggccaga
    12841 tcattaatgc cggcatggtc gactacaagg tgccgagctt cgatgacgtg cccgaccagt
    12901 tcgattccat tctcatcgaa gatgctaacg gtcctggccc ttacggcgca aagggtctgg
    12961 gtgaaggcgg gattattcct gttgccccgg taattgccaa cgcgatcgcc tggtgctcgg
    13021 gcgcgcgcat caagacgctg cctttgacgc cggaaaaagt ctggcgcgca atcaaggcga
    13081 cggaggaaaa ataagaggca cgatggcggg cagcaatgaa tttgaaaaaa aaagttcgcc
    13141 caacgttcga ggcaatcccg tcatcgactc gtcaactcag agggataagt tgaatcccaa
    13201 caattctcgc cacgaatttt tcgcatgcgc gcgctgccaa catcccgaag ggacacccag
    13261 cgccgctcca agacaatcga tgcgctacga gctttccgac aacacccac
//
LOCUS       DASUVS010000456        21111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1735864, whole genome shotgun sequence.
ACCESSION   DASUVS010000456 DASUVS010000000
VERSION     DASUVS010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 21111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21111
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735864"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4127)
                     /locus_tag="VF182_24000"
     CDS             complement(<1..4127)
                     /locus_tag="VF182_24000"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6440201.1"
                     /translation="MFWGFPHALLFLAGAVPLIVLLHSLKPKGIKLDTTTLFLWERVL
                     NEQPLSTRLGWLLRKNLLLILQIVAATMLIAALADPALRHFGALSGDTVIVMDLSASM
                     NARGSSGPRFEAARREFSSLIDGLGSHQRIMVIGAGAQPRLLQPFTADKRRLRQLARN
                     ISATDAPGRIKEAILFAHAFLKRDTPDRVVVISDGAFEGAEEYSRPADHLRFIKIEGG
                     NNNIGIVGFELRRHPERRSQVEVMVHVKNFTASSARVPLTLAIGEKVVMKEFVELEPD
                     GRQILIYSLENSPTGTLVARLEIDDDFATDNVAYLAFNDEPRVRLLYVGPGNPFLANL
                     FRFFPYLQINTAREWDPNAASSQYDVVVFDNVPAPPLVQGNFILINTMASNLPLRVNG
                     AVRSPRIVSQATKHSITEGVSLRDLQVRRSLLLALSGEGTVLARSPESPLIVAIETGK
                     LRALVIGFDLIDSDLPLRVAFPVLVHNTLEWFQPQQLEFPARRVQSGNPISLPLPASE
                     GDIELTTPSGRKEILSPATNPLIFADTSEAGLYTYKKNGAESRFAVNLLDESESQIAP
                     HVNLGSSAAEAANQISQKLDASLPLWPALLILALLVLAVELALAFRSGMPLSPVVIRA
                     AALAALMLAVLNPKIFQSATALDVVLDVDLSRSVGQEAREKAQEVLEVIRRARTPETR
                     TGMVVFGGAPEWEFLPRENLPATDAALRLDREQTDIQAALQASLAQIGEGRQGKILLI
                     SDGNENRGEISRIIPLLRSNGTQVWTLPVSLSQGRNEVYLSDLASPRQVDSGEGFEIA
                     GKIESLRNAAARIKLLRDGVLIRDQEIRLRSGTNQVSFRESLLERGNHSYELLLESRD
                     DTLAENNLLQGVVEVKGAPRVLLLSANNDSQRFLAKVLQVQGYSVVQSVPEKTPLTLT
                     DLASFDLLVLDNVPAFQLTDAKMENIEKYVRDLGGGLLVIGGSQSYGAGGYYRTALER
                     VLPVDMRPPARLDLPHVALLFVLDKSGSMGAGQEGSTKLDLAKAAAVAAADIMNPTDQ
                     VGIIGFDAAWDWVLPFRPVGNGEWISEKLATLQSDGGTDLYKALVEAHRAIAAKQAAI
                     KHVIVLSDGLTDKADFQGLGQRMARDGITVSTVSVGNDADVQLMADIAKEGKGRGYVA
                     LDPQTIPQIFTAETLLISRDLLVDKSFTPNIVAPIGPLKGIGQSGLPPLRGYVLTYPK
                     PRSELLMTADKDPLLVSWRYGVGRVMAFTSDLSGRWGKEWVTWPGFPQWASQLARDTM
                     RKFLETRMRAELQPDGEAVKVVADLLSKEGQFLNHLKLKSSIATPSKRTLEQALQQSA
                     PGRYETKFVPSQRGIHLLTLYAEGEPGHAGQPVGTIPYIAPYPKEYRELKPNTALL"
     gene            complement(4133..5098)
                     /locus_tag="VF182_24005"
     CDS             complement(4133..5098)
                     /locus_tag="VF182_24005"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX6440202.1"
                     /translation="MRFSNVVFPTRAVEENTIRQPAQTAPQEPAATFFDAEFLKKIER
                     LRLVAKRLSWAGAKGEHAVSRKGFSLEFSDYRNYQPGDDLRYVDWNIYRRLERLLVKV
                     FTAEEEMNIYLLVDTSRSMAEGSPAKIDYAKRVAAALGYIGLKNLDRLGGASFANTLR
                     TPLSLGRGRKQILRLFNFLGGLSCSGATDLRLSVHSFCRLFPHAGLVIVVSDLFDPAG
                     WRAALQELALKKHQILIVHIIDEQESSPRASGDIILRDVEGGHERKIFLDAELARRYQ
                     EELRRYLGEIEAICAGRQIDYLRTTTQVPFEEFVLKTLRQASSVN"
     gene            complement(5176..6162)
                     /locus_tag="VF182_24010"
     CDS             complement(5176..6162)
                     /locus_tag="VF182_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX6440203.1"
                     /translation="MESARRSFDEVKNEMARVIVGHQDLIEDIFIALVCGGHCLLEGV
                     PGLGKTLLVRSLGEILSLSFSRIQFTPDLMPADITGTNMLTDDPSGRKIFEFQRGPVF
                     AHIVLADEINRATPKTQSALLEAMQEQNVTVGGKLHRLEHPFMVLATQNPIEMEGTYP
                     LPEAQVDRFFFKLVLEFPTHAELSRIAELTTAAPPGPLREIVDGPAILQMGQWIRDVP
                     LAEIVKDYAVRIVLSTHPQRQPGNGAAMAQKFVQYGSSPRGLQSLILAAKARALFAGR
                     PNVSFDDIRAMLLPSLRHRLILNLEADAEGIGAEKILQEIVAQVPEVPAKSV"
     gene            complement(6258..7697)
                     /locus_tag="VF182_24015"
     CDS             complement(6258..7697)
                     /locus_tag="VF182_24015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440204.1"
                     /translation="MNEAPEILHRRFLQLFRWEKRKRRREILISLVCYSLLAALLLLP
                     IYFLLPASFLRWFVPAAIFSILAPFLFISRRWRREDSARALVDLDKALHSDERAITAW
                     ELIESKEHRPAALLVLEQAAERLKKVDPKSLFHRSRRWQDYALVPLLLVWGGLLWFDV
                     DVRLRPEVHVPTQQGLAQKLRQFSRELQEKAGSEGLRQSLQVGRELEKTAQKNIDAKV
                     ADQQFKNELSGMSSKVEAMGKSIGNQPTFATSESSQSLKDLKAELEAAEELLNLPDSA
                     KGESTVGQASLDRLANLPQLKRQLDRQGEANRPLNQDELKSFLNSMEKQVTGELDRRT
                     LLDAQQFLEQLAKQEPGEKAEANARVAGRGEQDLPGDGEKAKSNSNRPGSEPGTKEEI
                     QPAPELPAAAATQLKGMIGQGDSSGVGLKGKPSAGKTEVPQEEVIATYLRQAEQDLDS
                     ERVPEALKETIRRYFLSLEAKQRGDDSGK"
     gene            complement(7694..8440)
                     /locus_tag="VF182_24020"
     CDS             complement(7694..8440)
                     /locus_tag="VF182_24020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440205.1"
                     /translation="MKSNPVLRESIDIFFVEGHGFAVYFYILVILAPVEFLSLYLPSL
                     DAQMWSGSASLFKVTAVTTLLLTIYFAMRVANQEFASWRFRTLKRWHRENGQPVAAIA
                     QAQLMFLSLHVALSMLLCAPLLIWAAGIARTPFSTVALTFGLLFFYAWGYAIWGLFAL
                     TLWERRAESRQVFIRSFFFTLTILSLLLYLPLNPVAFLLAVLSRQEYPALSIAGYQWH
                     AGTIHFFVHLLFIGLALLAHRWALRRELGQ"
     gene            complement(8437..10266)
                     /locus_tag="VF182_24025"
     CDS             complement(8437..10266)
                     /locus_tag="VF182_24025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440206.1"
                     /translation="MGFHGIFQLGRPFPLELEVSNSGRPADGFLEVQVWKGGAAKSGV
                     PYPLYYRRDLFVSGQSRKTIQLTVDPDFISRPLVISFISPAGNASRELDLRRYFSPTP
                     VMLLVSEASSLSISLGSALPNRLVAVSLSELPSDPRALLGVSHLFVYDQSLRELSRAQ
                     LFALESWLTAGGRMVIIGSLNYALYQETSLGRFLPVRVTGSQRISFSPQTIEDEPTTP
                     IAGVWAQVSSLLDGKVLLESAGIPLLVERSRGKGRITYFALDLGRPPLAQWEGLPKLL
                     RKLVIPAGEDALPPRTRWDDAIFSQIIVSPSFISTYVPTRSLLLALIGYFAALGMLAW
                     LWQKSRLPARKMVIGLAMVVAVAAAAGYPLFSRGGNVPDGVLLSSTMLDNVTDGYVEA
                     QLNMAMFSTQIRQYNLQLAPGWLEMSPVSIPARDRPQEAVLLQDGNGSSRFRLPLREW
                     DYRLFRMRFIDRFPMRAEFEIQGDKLLMKIDNQSSKDLVDCWLALPGGRHSLGEIARG
                     SRWSKVFPLNTTESSETNQSSRGDALSLRDLSFNDKTRDILFHSSFFPRDGETSRWNT
                     GTAIFFGWVKDSERRVNVDDPRIHARDYTLFRVIIPLPGPEDE"
     gene            complement(10507..10995)
                     /gene="greA"
                     /locus_tag="VF182_24030"
     CDS             complement(10507..10995)
                     /gene="greA"
                     /locus_tag="VF182_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX6440207.1"
                     /translation="MATQDERVPMTTRGYQSLTDELKRLKSVERPKNVREIEEARGHG
                     DLSENAEFHAAKERQSLLDIQIREIEDRLARAQVIDVSTLSGEKVVFGATVALADGDT
                     GDKVVYQIVGDHEAEPKNGKISISSPVARALIGKSEGDEVQVQTPTGVRSFEILNVEY
                     ID"
     gene            complement(11185..12162)
                     /locus_tag="VF182_24035"
     CDS             complement(11185..12162)
                     /locus_tag="VF182_24035"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX6440208.1"
                     /translation="MSLLEIRNLKKHFPVGQALFSRNKDVVKAVDGVNLNVEQGETLG
                     LVGESGCGKSTLGRAILRLIEPTSGEVYFDGKNLLAMSQRELRDMRRQMQIIFQDPYA
                     SLNPRMRVGDIVGEGLEIHKIAKGKAKRDRVIELLRQVGLREDHYGRYPHEFSGGQRQ
                     RIGIARALGVGPKFIVCDEPVSSLDVSIQAQIINLLQELQEKMHLTYLFISHDLRVVE
                     HISHRVAIMYLGKVVETAQSETIYRDAKHPYTRALLSSVPIPEVDGKKERVLLQGDVP
                     SPVNPPSGCAFHPRCAYRAAVCDQMEPPLDFDADHHGVACHVFGPVKRA"
     gene            complement(12210..13235)
                     /locus_tag="VF182_24040"
     CDS             complement(12210..13235)
                     /locus_tag="VF182_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6440209.1"
                     /translation="MAELIKVKDLHTSFFTPEGEVRAIDGISFEIDEGKTLGLVGESG
                     CGKSVTSLSIMRLIASPPGKIVGGEILYRGRDLLKFKKEEMRKIRGNEISMIFQEPMT
                     SLNPVFTVGNQIGEAIRLHQGLGKKETRQKTIDMLRLVKIADPESRVDAYPHQLSGGM
                     RQRVMIAMALSCNPSLLIADEPTTALDVTIQAQILDLMKELQQRLGMALLLITHDLGV
                     VAEQADEVAIMYGGKIVERASARAIFTRPFHPYTVGLLNSLPGTGRLKKKRLDAIPGV
                     VPNPLHLPSGCRFRDRCPRAEDLCARVEPQLLEKEPGHTVACHFPHTEWNPKFERETE
                     MQRQGSA"
     gene            complement(13579..14607)
                     /locus_tag="VF182_24045"
     CDS             complement(13579..14607)
                     /locus_tag="VF182_24045"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440210.1"
                     /translation="MRKSMRIMLVFVGINFFIALACVTLQAASAPRKFVIGHAAMNAR
                     VAPLWVAEDVGFFAKHGVSASAIFIRQAPVLVAALTAGDVHAAYTGGTTVLTAVAGGA
                     DLKIIGSLTNRLGYDMVAAPNIKTPIDLRGKRFGVQTLAGTVWLGAMLGLEHLGLEAK
                     RDNITFLVIGDQTVATQALEGGQIDVTVLDGVFSRRLKQKGFTILAELQKENIPFSSQ
                     GLVARGAFLQEQRETMESVMKGLLESIAFCLAPKNKNAVLATITKRLKLADPSVAEEG
                     YQDIINGVERKPYPTVEGLRNIQRLMKIRNPALDKVKVEDMIDDRILRRLDESGFIDQ
                     LAKTYPAK"
     gene            complement(14723..15493)
                     /locus_tag="VF182_24050"
     CDS             complement(14723..15493)
                     /locus_tag="VF182_24050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6440211.1"
                     /translation="MKLQNQVAVITGAGRNIGKAIAKLFADEGARIAVIEMHEGRGQA
                     VVQELENSGHEALLVLCDVANSSDVQQMVQKVVARFGGIDILINNAALTDHADIFTST
                     EDEFDKVIAVSLKGPYLVTKHIAAQMAKQGRGGKIVNFGSTSGMLGRTDGIAYAAAKG
                     GVINLTRAMAVQLSPYRIRVNCVVPNRSGSPVGYDDDVAGSRTFQNLAGRLGTAEDQA
                     KAALFLVSEDSDFIYGHPLVVDGGVVATANFPKEQFVK"
     gene            complement(15568..16116)
                     /locus_tag="VF182_24055"
     CDS             complement(15568..16116)
                     /locus_tag="VF182_24055"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6440212.1"
                     /translation="MARIAAIEKKEDLAPGDQSIYDSIAQSRGVVGGPFLALLHSPEL
                     AARTAHLGSYVRFESSLEPKIIELAALVSARELQCKHEWAAHVNHAQKAGISLDIIRA
                     VHQGKDAEDFTFEDAQIVSFVREVLRAHRVNETTFQALYARLGERGLVELTATVGYYA
                     MLACTLNTFDIATASAPDEFKI"
     gene            complement(16126..17253)
                     /locus_tag="VF182_24060"
     CDS             complement(16126..17253)
                     /locus_tag="VF182_24060"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6440213.1"
                     /translation="MAEKSLEEIKADILHRAGRINPFERVAREDVKQVLANLNSLDPD
                     LWGSEWGKLGAKYEALADEQETTGRSTQAGETYYLAYEYYRIGRYPVPSSPQKMACYR
                     GALRSFLKAAPTMNPPLRRIEIPFEGKKVVGYLQIPKELDRPPVVLHWGGVDGWKEDR
                     RTNNDILLKKGLACLTMDMPGTGENPCKANDPRAERTFSAAIDYLETRDEIDGKRVAV
                     MGGSFGGYWAAKLAHTEATRLRGAVNWGAGVHRTFQEEWLRPALTLTASQYLMGPASL
                     LDARSYIFGVKSLEEVLKIAPTLSLKTQGLLDQPCAPLLCINGKEDDQHPVDDIYLLL
                     EHGSPKDVRIIADAGHMGRKKGQSNDDVVRIVTTWLQDKLA"
     gene            complement(17405..18385)
                     /locus_tag="VF182_24065"
     CDS             complement(17405..18385)
                     /locus_tag="VF182_24065"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440214.1"
                     /translation="MKSSDIFALTFGIVLFSLPLQAQISKPVYVSLPGPGNVQHLAYI
                     VAKEKKFYEEMGLTNVNIVVLRGNAMNVQALMSGSVHYSSAFGPSMQAMFRGEPVRVL
                     LQIFNQIPFGLIVRPEINRLDDLKGMKVAVTFGGSTYSVLQALFAKYGFSDKFAEYLN
                     IPDNEGKAIALMQGRASAALMAPPTDRPLLKAGMKRLVYLGDEFKNVPFSALQAMAKH
                     VQSEPEMTERMVKAVMKALYWIRANREGSIDIIMKNGRLNERDIAASLYDLMRDAYIP
                     ALTPEGLYKRAELEFVLLKEKPNFKPEQFIDDRFFKAALKALANEPGTKS"
     gene            complement(18436..19500)
                     /locus_tag="VF182_24070"
     CDS             complement(18436..19500)
                     /locus_tag="VF182_24070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6440215.1"
                     /translation="MSALQSNQAAPEEIGSAESATSVGLWRDVWKRFKRNSIALTGFY
                     VVCILACMALSADFLANDKPYYFRYGGKAYFPILRSYMVAAGVGHWPPELLNVDFKKV
                     EGAQALFPPVPYRPTNINLLEPLEPPSGKHWLGTDKLGRDVMSGMIHGARISLSIGFV
                     AVGIAVIIGLVLGAIAGYFAGWVDLIISRLFEIMLSIPTFFLLITVAALLPPSIFLTM
                     AIIGATSWVGIARFTRNEFLRIRNQDYVTSAIALGVTDRNVMFKHILPNALAPVIVSV
                     VLGIAGAILVESSLSFLGIGVPAELVTWGSILQEASTTTFAWWLAVFPGFAIFITVLA
                     YYLVGEGLREVLDPRLQGLR"
     gene            complement(19497..20528)
                     /locus_tag="VF182_24075"
     CDS             complement(19497..20528)
                     /locus_tag="VF182_24075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6440216.1"
                     /translation="MTGYLIKRLLLMVPTFIGITLIGFLIMRLAPGDPAELRAAGGLG
                     AAAGAGISVEKRGSVDEAIAQWRAQYGLDKPLHVQYLVWLKNIGTLNFGESFKDNQSV
                     WSKIAERLPVTVKLNFWSLLVVYLVAIPLGVYSATHPNSFGDQVTTLAAFVLFAVPLV
                     WAATMAIVFICGGDFYYLFPPGGLESIDFSYNWPIWRKLQDRAWHIFLPVVLLSYDGF
                     AGLSRYMRASMLEVLAQDYVQVARAKGLPEKRVILKHVLRNSLIPQVTILASILPGLI
                     GGSVIIETIFSIPGLGQLGFESVLARDYPTVLALFTVSALLTLTGILISDLLLAAVDP
                     RIVFGRRQQ"
     gene            complement(20525..>21111)
                     /locus_tag="VF182_24080"
     CDS             complement(20525..>21111)
                     /locus_tag="VF182_24080"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440217.1"
                     /translation="EAGWKDTDGDGVLDKVIDGNRIPLRFEIKINAGNTVRESVALVL
                     MDELNKHGIAASVRQLDWTIFLNDVKNHQFDAVVLGWAMSTAEPDAYQVWHSSQAANK
                     GSNAISYKNPRVDEILEHYRQEFDPQKRIELYKEFQQILHDEQPYTFLHVRKTVSAVH
                     RRFQGVEVFPDGLKPIDWWVPLAQQKYANSIAPQ"
BASE COUNT         4763 a         6095 c         5681 g         4572 t
ORIGIN      
        1 aggagggccg tgttgggctt cagttcacgg tattccttcg gatagggagc gatgtaagga
       61 atcgttccca ccggctgacc tgcgtgtccc ggctctcctt cggcatagag cgtcagcaaa
      121 tggatgccgc gctgtgatgg cacgaacttg gtttcgtatc gtcccggcgc gctttgctga
      181 agcgcttgct cgagggttct tttacttggc gtcgcgatac tgctcttgag cttgaggtga
      241 tttagaaatt ggccttcttt ggacaacaaa tcggcaacga cttttaccgc ctcaccgtcc
      301 ggttgtaact cggcgcgcat tctggtttca agaaacttgc gcatggtatc ccgggccagc
      361 tggctagccc attgcggaaa gcccggccac gtaacccatt ctttgcccca acggccgctt
      421 aaatccgagg taaacgccat cacccgcccg acgccgtagc gccacgacac caggagcgga
      481 tctttatccg cagtcatgag cagttccgag cggggtttcg gataggtgag aacgtagccg
      541 cgcagcggcg gcaatccgct ctggccgatg cctttcagcg ggcctatcgg cgcgacgatg
      601 tttggcgtga aagatttgtc caccagcaga tcgcgcgaga tcagcaaggt ctccgcggta
      661 aagatctgcg gaatcgtttg cgggtctaac gccacatagc ctctgccttt gccttctttg
      721 gcaatatcgg ccatgagctg gacgtcggcg tcgttgccaa ctgaaacggt cgaaacggta
      781 atgccgtcgc gcgccatccg ctggccgagc ccctggaaat ccgctttgtc ggtaagtcca
      841 tccgagagaa caatcacgtg cttgatggcg gcttgcttgg cggcgatcgc gcgatgcgcc
      901 tccaccaagg ctttgtaaag gtcggtgccg ccgtcggact gcagcgttgc gagcttttcg
      961 gaaatccatt ctccattgcc taccggacga aacggtagca cccaatccca ggcggcatcg
     1021 aagccaataa tgcctacctg gtccgtcggg ttcatgatgt cggcagccgc aaccgctgcg
     1081 gccttggcta agtcaagctt ggtgctgccc tcctgtcccg cacccatgct gccggatttg
     1141 tcgagaacaa agagcagcgc gacatgaggc aaatcaagcc gcgcaggcgg gcgcatgtcg
     1201 accggcagaa cccgctccaa cgctgtccgg tagtagccgc cggcgccata actctgcgaa
     1261 ccgccgatga cgagcaagcc gccgcccaaa tcgcgcacgt acttttcaat gttttccatt
     1321 ttggcatccg tcaattgaaa cgctggcaca ttatcgagca ccaaaagatc gaaggaagcc
     1381 agatcagtga gagtcaacgg ggttttttcc ggcaccgatt gaacgacgga atagccttgg
     1441 acctgcagca ctttggcgag aaatcgctgg ctgtcattgt ttgccgaaag gagcaatacc
     1501 cttggcgcgc ccttcacttc aaccacgcct tgcaataaat tgttctcggc aagggtatcg
     1561 tcgcgtgact cgagcagcag ttcatagctg tggttgccgc gttccaacaa gctttcgcga
     1621 aagctcacct gattggttcc ggagcgtaat cgaatctctt ggtcacggat caagacgcca
     1681 tcacgcaaga gtttgatgcg cgcggccgca ttccgaaggc tttcgatctt gccggcgatt
     1741 tcgaatcctt cgccgctgtc tacctggcgt ggagaagcaa gatcgctgag atagacctca
     1801 ttcctcccct gcgacaggct tacgggaagg gtccagacct gagtgccgtt cgaacgcagg
     1861 aggggaataa tgcgcgagat ctcgccgcga ttttcattgc cgtcggagat gagaagaatc
     1921 ttgccctggc gcccctcgcc gatttgcgcc aacgaagcct gcagagctgc ctggatgtcg
     1981 gtctgttcgc gatccaaccg caatgccgcg tccgtggcag gtaaattctc gcgcggtaga
     2041 aactcccatt cgggcgcgcc gccaaacacg accatgcccg tacgcgtttc cggtgtcctc
     2101 gcgcgcctga tgacttcgag cacctcctga gccttttccc tggcctcctg tccaacactg
     2161 cgcgagagat cgacatcgag gacgacgtcc agagcggtgg ctgattgaaa aatctttgga
     2221 ttgagaaccg ccagcatcaa cgcggccaac gctgccgcgc gaataaccac cggtgataac
     2281 ggcatgccgc tgcgaaacgc cagagccaac tcaaccgcca gcacaagaag cgccaggatc
     2341 aagagcgccg gccataacgg taaactagcg tcgagcttct gactgatctg attggcagct
     2401 tcggcggcgc tgctgccgag gttgacatgc ggcgcgattt gcgactcact ttcatccaaa
     2461 agattgaccg cgaagcggct ttctgcgccg ttttttttat aagtgtaaag acccgcttcg
     2521 gaggtgtcgg caaatatcaa gggattggtt gccggactga gaatctcctt cctgcccgac
     2581 ggggtcgtta attcgatgtc gccctcgcta gccggaagcg gcagcgagat cggattcccc
     2641 gattgcacgc gccgtgcagg gaattccagt tgctggggct gaaaccactc cagggtatta
     2701 tgaaccagca ctggaaacgc cactctaagc ggcagatccg agtcgatgag atcgaagccg
     2761 atcaccaacg ctcggagttt gcccgtttcg attgccacga taagcgggct ctccggcgaa
     2821 cgggcgagaa cagttccttc gccgctgagc gccaagagga gtgatctgcg cacctgcaga
     2881 tcacgtagac ttacgccttc ggtaatggaa tgctttgtcg cttgagaaac aattcgcggg
     2941 ctcctgaccg ccccattgac ccgcaacggc agattcgacg ccattgtgtt gatcaagata
     3001 aagtttcctt gcaccaaggg cggcgcgggc acattgtcga aaaccacgac gtcgtactga
     3061 gatgacgctg cattcggatc ccactcgcgc gcggtgttga tttgcagata aggaaaaaag
     3121 cggaacagat tcgctaaaaa cggattacct gggccgacgt aaagaagtcg cacgcgcggc
     3181 tcatcgttga atgccagata agcgacattg tcggtggcaa aatcgtcatc gatctcgaga
     3241 cgagcgacca gcgttcccgt cggcgagttt tcaagagaat agatcaagat ctgccggcca
     3301 tcgggctcca gctcgacgaa ttctttcatc accacctttt cgccaatagc gagcgtcagg
     3361 ggaacacgcg ccgagcttgc ggtaaaattc ttgacgtgca ccatgacttc gacttgcgac
     3421 cggcgctcag gatgacggcg taattcgaac ccgacaatgc cgatgttgtt atttccgcct
     3481 tcgattttaa taaaacgaag atgatctgcc ggacggctat actcttcagc gccctcgaac
     3541 gcgccgtcac tgatcaccac gacccgatcc ggagtgtccc gcttgagaaa cgcgtgagca
     3601 aataaaatcg cttctttgat ccggccggga gcgtcggtgg cgcttatgtt tcgcgccagc
     3661 tggcgcagcc ttcgcttatc cgcagtaaac ggttggagaa gccgcggctg cgccccggca
     3721 ccgatcacca tgattctctg atgcgacccc aagccgtcga tcagtgacga aaactcgcgc
     3781 cgcgccgcct cgaaccgagg gcccgagctg cccctggcgt tcatactggc gctcaagtcc
     3841 atcactatca cagtgtctcc ggataatgcg ccgaaatggc gcaacgcggg atcggcaagc
     3901 gcggcgatga gcatcgttgc ggcaacgatt tgtaaaataa gcagcaggtt cttgcgcaac
     3961 agccatccca gccgggtcga cagaggctgc tcattgagca cacgttccca gagaaacagt
     4021 gtggtggtat cgagcttgat ccctttaggt ttaagactat gcaggagaac aatcagcggg
     4081 acagccccgg cgagaaacag taaggcgtgt ggaaaacccc agaacatctt ttttagttca
     4141 cgctgcttgc ctggcgcaac gttttcaaaa cgaattcttc aaacggcact tgcgtggtgg
     4201 tgcgcaggta atcgatttga cggccggcgc agatagcttc gatttcaccg agatagcggc
     4261 gcagttcctc ctggtagcgg cgcgccaact ccgcatccaa gaaaatttta cgctcatgcc
     4321 cgccttcgac atcgcgcaag atgatgtccc ccgaggccct gggactgctt tcttgttcgt
     4381 cgatgatgtg aacgatcaaa atctggtgtt ttttcaatgc gagttcctga agggcggcgc
     4441 gccagccggc cggatcgaac agatcactga cgacgatcac caagccggca tgaggaaaca
     4501 gacgacagaa cgagtgcacg gacaggcgca agtcggttgc gccgctgcag gacaggccgc
     4561 cgagaaagtt aaaaagacgc aggatctgct ttcgtcccct acccaaactc agcggcgtgc
     4621 gcaacgtatt ggcaaacgaa gcgccgccga gccgatccaa atttttcagg ccgatgtagc
     4681 ccaacgcggc ggcaactctt ttggcataat cgattttcgc cggcgatcct tcggccatcg
     4741 agcggctcgt atcgaccaag aggtagatat tcatttcttc ctctgcggtg aacactttca
     4801 ccaacaagcg ctcgagccgc cggtagatgt tccaatccac gtaacgcaaa tcgtcgcctg
     4861 gttgatagtt gcggtagtcg gaaaactcca ggctaaagcc ttttcgtgaa acagcgtgct
     4921 cgcccttggc acccgcccaa ctgagtcgct tggcgaccaa ccgcagccgt tcgatttttt
     4981 ttagaaattc cgcatcgaag aaagtcgctg cgggttcctg cggagcagtc tgagcgggct
     5041 gtctgatggt attttcttct actgcccggg tcggaaaaac cacattagag aaccgcatcg
     5101 acgggatcgc gtcccgtatc gatgcgcagg actgtttcaa gcgaattcga aaattttcaa
     5161 agcgaaatcc ggaattcata cacttttggc tggaacctcg ggaacctgcg cgacgatctc
     5221 ctggagaatc ttttcggcac cgatcccttc ggcatcggct tccaagttta atatgagtcg
     5281 atggcgcagg ctgggcagca acattgcgcg aatgtcatcg aaggagacat tgggccttcc
     5341 agcgaagagc gcccgagctt tggcggccaa tatcaagctc tgcaggccgc gcggactgga
     5401 tccgtactgg acgaattttt gcgccatggc agcgccgttg cctggctgtc tttgcggatg
     5461 ggtactgaga acaatccgca ccgcataatc ctttactatt tcggccaagg ggacgtccct
     5521 gatccactgc cccatttgca aaattgccgg gccatcgaca atctcgcgca gcggaccagg
     5581 cggcgcggcc gtggtgagtt cggcgatgcg gctaagttcc gcatgcgtcg ggaactccag
     5641 caccagcttg aagaaaaagc gatctacctg agcttcagga agcggatagg ttccttccat
     5701 ctcgattgga ttttgcgtcg ccaggaccat gaacggatgc tccaagcgat gcagtttgcc
     5761 gcccaccgtg acgttttgct cttgcatggc ttccaataaa gcggactgtg ttttgggagt
     5821 ggcgcgattg atctcgtcgg ccaggacaat gtgagcgaat accggaccgc gctgaaactc
     5881 aaaaattttt ctgcccgacg gatcgtcggt gagcatattg gtgccggtga tatcagccgg
     5941 catcaaatcg ggcgtgaact gaatgcgtga gaaactcagg cttaggatct cgccgagact
     6001 gcgcaccaat aatgtcttgc ccaatccggg gacaccttcg agtaaacaat ggccgccgca
     6061 aacgagcgcg ataaagatat cctcgatcag atcctgatgg ccgacgatca cccgcgccat
     6121 ctcgttcttg acttcatcga aagagcggcg ggcactctcc atacgctgtt caatttctgc
     6181 agaggattcc acgatcaact cctagcttca tgcgcccatg ccggcagctt aatcttgcct
     6241 gcatgctact aacttagcta cttccccgaa tcatcgccgc gctgcttggc ttccaaagac
     6301 aagaaatatc gtcttatagt ctccttcagc gcttcgggga cacgttcgct gtcgaggtcc
     6361 tgctcggcct gtcgcaggta agtggcgatc acctcctcct gagggacttc ggtcttcccg
     6421 gccgacggct ttcctttcaa tccgacaccg ctgctatcgc cctggccgat catacctttc
     6481 agctgagtcg cggcagccgc aggcagctct ggcgccggct gaatttcctc tttcgttccg
     6541 ggttcgcttc ccggccggtt gctgttgctc tttgcttttt caccgtcacc cggcagatcc
     6601 tgctcgcccc gtcccgcaac tcgggcgttg gcttcggcct tttcaccggg ttcctgttta
     6661 gctaattgtt cgaggaattg ttgagcatcc agtagagtgc gccggtccag ctcgccggtg
     6721 acctgtttct ccatggagtt gagaaatgat ttcagctcat cttggttgag tggccggttt
     6781 gcctcaccct gacgatcgag ttgccgcttg agctgcggca agttcgccaa tcggtcaagg
     6841 gatgcttgtc cgactgtgct ttctcccttg gcgctatcag gcaggttcag cagctcctca
     6901 gccgcctcca gttctgcttt aagatctttc aggctttggc tgctctccga cgtggcaaac
     6961 gttggctgat tgccgatgga ttttcccatc gcttcaacct tgctgctcat tccactaagt
     7021 tcatttttga attgttgatc ggcgactttc gcgtcgatat ttttctgcgc cgttttttca
     7081 agttcgcggc cgacctgcag gctttgtcgc agcccttcgc tgccggcctt ttcttgcagc
     7141 tcacgcgaga attgccgtag cttttgcgcc agcccctgct gcgtcggcac gtgcacctca
     7201 ggcctgagcc ggacatcgac atcgaaccag agaagaccgc cccaaacgag caagagcggg
     7261 actaacgcgt agtcctgcca gcgccgtgag cggtggaaca gtgatttggg atccactttc
     7321 ttcagacgct ccgccgcttg ctccagaacc agaagcgcgg cgggccgatg ctcctttgat
     7381 tcgatcagct cccaagcggt tatcgcccgc tcgtccgaat gcagggcctt atcgaggtcg
     7441 accagggcgc gcgccgaatc ttcccggcgc caacgacggg atatgaataa aaatggcgct
     7501 aagatcgaaa aaatcgccgc tggtacaaac cagcggagaa agcttgccgg aagcaggaaa
     7561 tagatcggca gtaacaacaa cgccgccaac agtgaatagc acaccaggct gatcaggatc
     7621 tcccgcctcc ttttgcgctt ttcccaacga aataattgga ggaatcttcg atggaggatc
     7681 tccggcgcct cgttcattgg cccaactccc tccgcagcgc ccaccggtgt gcgaggagcg
     7741 ccaaaccgat gaacagcaaa tggacgaaaa aatgaatggt tccggcgtgc cattgatatc
     7801 cggcgatact tagcgccgga tattcctgcc gggaaagaac ggccaagagg aacgccacgg
     7861 gattgagcgg caagtagagg agcagcgaga ggatcgtcaa ggtaaagaaa aaagagcgga
     7921 tgaatacttg acggctttcg gcgcggcgct cccacaatgt taatgcgaac agcccccaga
     7981 tcgcgtaacc ccaggcgtag aaaaacaaca gcccgaaggt aagcgccacg gtcgaaaacg
     8041 gcgtgcgcgc aataccggcc gcccagatca acaatggcgc gcacaggagc atcgaaagcg
     8101 cgacatgcaa cgatagaaac ataagctgcg cctgagcgat ggcggcgacg ggctggccgt
     8161 tttcgcgatg ccagcgcttg agtgtccgaa atcgccagga agcaaactcc tgattcgcca
     8221 cccgcattgc gaagtaaatg gtcagcaaca acgttgtcac cgcggtgacc ttgaacagac
     8281 tcgccgaacc gctccacatc tgggcatcca gcgagggcag atagagcgac agaaactcaa
     8341 ccggcgcaag aatcaccaaa atatagaaat aaaccgcaaa cccatgcccc tcgacaaaaa
     8401 aaatatcaat actttcgcgc aaaaccggat tgctcttcat tcatcctccg gtccaggcaa
     8461 tggaatgatc acccgaaaca gcgtgtagtc gcgggcgtgg atgcgcgggt catcgacgtt
     8521 gactcgccgt tccgaatctt ttacccagcc gaaaaagatc gccgtgccgg tgttccaccg
     8581 cgacgtctcg ccgtcgcgcg gaaaaaaaga agaatgaaac aagatgtcac gggttttgtc
     8641 gttaaacgac agatcgcgca gactaagggc atcgccgcgg ctcgattgat tcgtctcaga
     8701 cgactctgtc gtgttcaatg gaaaaacttt gctccatcgt gaaccgcgcg cgatttcccc
     8761 tagagaatga cgcccgccgg gcaatgccaa ccagcaatcc acgagatctt tgctgctttg
     8821 gttgtcgatc ttcatcagca gcttatcgcc ttgaatttca aattccgcgc gcattggaaa
     8881 gcgatcgata aagcgcatgc gaaagagccg ataatcccac tcgcgcagcg gtaagcgaaa
     8941 gcggctggaa ccgttgccat cctgcagcaa caccgcttct tgcggccgat ctctggccgg
     9001 gatcgatacc ggtgacattt cgagccaacc aggagctagt tgaaggttgt actggcggat
     9061 ttgagtcgaa aacatcgcca tgttcaattg agcctccacg tagccgtcgg taacattatc
     9121 gagcatggtg gaggagagca gcactccgtc agggacattc ccgccccggc taaacagcgg
     9181 atatcccgcc gccgcggcaa ccgcgacaac catcgccagg ccgatgacca ttttgcgcgc
     9241 cggcaaacga ctcttttgcc agagccaagc taacataccg agagccgcga aatagccgat
     9301 taaggccagc aacaaggaac gggtgggcac ataggtcgaa ataaatgacg gactgacgat
     9361 gatctgagaa aagatcgcat catcccaacg agttcgcggc ggcagcgcat cctccccggc
     9421 gggaatcaca agcttgcgca aaagcttggg caaaccttcc cattgagcga gtggaggtcg
     9481 tccaagatcg agggcaaaat aggtgattcg gcccttgccc cggcttctct caacgagaag
     9541 cggaattccc gctgattcca ggagaacttt gccatccagc aagcttgaaa cctgtgccca
     9601 gactccggcg atcggggttg tgggttcgtc ttcgatcgtt tgcggagaga aagagatcct
     9661 ctggctgccg gtgacccgca cagggagaaa gcgaccgagg gatgtctcct gatagagggc
     9721 atagttaagg ctgcctatga tgaccattct gccgccggcg gtgagccagc tttccaaggc
     9781 aaacagctgc gcccgcgaga gctcgcgcaa ggactggtca tacacaaaca gatgagaaac
     9841 cccgagcaga gctctcggat ccgacggtag ctcgctcagt gaaacagcaa ccaaccgatt
     9901 gggcagcgcc gagcccagag atatggagag cgaactggct tcgctcacca gcagcatgac
     9961 gggggttggc gagaagtagc gccgcagatc cagctcacgt gacgcgttcc ccgcggggct
    10021 gataaaagag atcaccagcg ggcggctgat aaaatcggga tcgacggtaa gttggatggt
    10081 tttccgcgac tggccggaga caaaaaggtc tcggcgataa taaagcggat aaggaacgcc
    10141 gctctttgca gcgccgcctt tccaaacctg aacttccaaa aagccgtcgg ccggacggcc
    10201 agagttgctg acttctagtt ccagcggaaa cggacgtccc agctgaaata tcccgtgaaa
    10261 gcccacacgc ggctcgatga gtatctcagc gtaactaaga gatgtgaaaa gaaatgttaa
    10321 caggaaggag caaagcagag cggttagtcg cgggcgggcc ggaattgatt gcgaaaagtt
    10381 gggcagcgcc aaaaccgccc ggtgaggcca atctgctatc tggccgcggt taaaaccttg
    10441 agctgccttt cggactgaaa aggacatgat ccctttccag aaaggcccgc cccaccacag
    10501 cagagatcaa tcgatatact cgacgtttaa gatctcgaat gaacgcacgc ccgtgggggt
    10561 ctggacctga acctcgtccc cctcgctttt gccgatcaaa gcacgcgcaa cgggagaact
    10621 aatagaaatt tttccgttct tcggctccgc ctcatgatca ccgacgatct ggtataccac
    10681 cttgtcgccg gtgtcgccat cggcgagagc gaccgtcgca ccgaaaacca ccttctcgcc
    10741 ggacagtgtt gagacatcga ttacctgagc ccgcgctagc cggtcctcga tctcgcgaat
    10801 ctgtatatcc agcaaggatt ggcgttcctt ggccgcatga aactcggcgt tttcagaaag
    10861 atcaccatgc cccctcgcct cttcgatttc gcgaacgttt ttaggccgct cgacactctt
    10921 caggcgttta agttcatccg tcaaggactg gtaaccccga gtagtcatcg ggacacgctc
    10981 atcctgtgtt gccatacatt aactcctata caaaacaaaa cctctctgga ttggctcctg
    11041 agaggctttc ggtcgatcgc tttaccttct tacccgtgaa gcgccgctac caaccggatg
    11101 gtaacatcct ccgcggttaa ttcaactctc tcgggaagca cttccaatgg accatcgagc
    11161 tggcgcaaga tagacgacta cggatcaggc gcgttttaca ggaccgaaaa catgacaggc
    11221 gacgccgtga tgatcggcgt cgaaatccaa cggcggctcc atctgatcac aaaccgccgc
    11281 gcggtaagcg caacgcggat gaaaagcaca ccccgacggg gggtttaccg ggctgggcac
    11341 atcaccttgc aacagcaccc gttccttttt cccgtccacc tcgggaatcg gcaccgacga
    11401 tagcagcgct cgcgtataag gatgcttggc atccctataa atggtctcgc tctgggctgt
    11461 ttccacgact ttgcccaaat acatgatcgc aacccggtgg ctgatatgct cgaccactct
    11521 cagatcgtgc gagataaaca gataggtcaa atgcatcttc tcttgcaatt cttgcaacag
    11581 attgatgatc tgcgcctgaa tcgaaacatc cagcgaagac accggctcat cgcaaacgat
    11641 aaatttggga ccgacaccca gcgcccgcgc gatgccgata cgttgccgtt gaccgccgct
    11701 gaactcatgc ggataacggc cataatggtc ttcgcgcaaa ccgacttggc gaagcagttc
    11761 gatcactcga tctcgtttgg ccttcccctt ggcgattttg tgaatttcca ggccttcgcc
    11821 gacgatatca ccgacgcgca tccgcggatt gagagacgca tagggatctt gaaagatgat
    11881 ttgcatctgc cgccgcatat cgcgcagctc gcgctgcgac atggccaata agttcttacc
    11941 gtcgaagtaa acttcaccgc tggtgggttc aatgagtcgc aagatggccc ggcctaacgt
    12001 tgacttgcca caaccagact ccccgaccaa acccagcgtc tcgccttgtt cgacgttaag
    12061 attaacgccg tccacggctt tgaccacgtc tttattgcgc gaaaataacg cttgccctac
    12121 tggaaaatgc tttttgagat ttcggatttc taacagactc atggtgatac cggattagat
    12181 cgaacagaga gaagtcgggt tggctttttt catgctgatc cctgtctctg catttctgtt
    12241 tcccgctcaa actttgggtt ccattcagtg tgcgggaaat ggcaggcaac ggtatgccct
    12301 ggttctttct ccaggagctg cggttcgacc cgggcgcata gatcttccgc cctaggacag
    12361 cggtcccgga aacggcagcc actcggcaga tgcaacggat tcggcaccac cccgggaatt
    12421 gcgtccaggc gctttttctt caaacggccg gtgccgggga gcgaatttaa tagccccacg
    12481 gtgtagggat gaaagggtcg ggtgaaaatc gcccgggcac tggcgcgctc gactatcttg
    12541 ccgccataca taatcgcgac ctcatccgct tgctccgcaa caacgcccag atcgtgggtg
    12601 atcagtagca gcgccatgcc cagccgttgt tgcaactcct tcatcaaatc cagaatctgc
    12661 gcttgaatgg tgacgtcgag cgccgtggtc ggctcgtcgg cgattaacaa gctcggattg
    12721 cacgatagcg ccattgcgat catgactctc tggcgcatcc cgccgctcaa ttggtgcgga
    12781 taggcatcga cccgggactc gggatcggcg atcttgacca ggcgcagcat gtcaatggtt
    12841 ttttgccggg tttccttctt tccgaggccc tgatgaagcc ggatggcttc gccgatctga
    12901 ttgccgacag tgaacaccgg attgagcgag gtcatcggct cctgaaagat catcgagatt
    12961 tcgttgccgc ggatcttgcg catctcctct ttcttaaact tgagtagatc gcggccccga
    13021 taaagaatct ccccacctac gatttttccc ggcggcgagg cgatcagtcg catgatggag
    13081 agcgaggtca cactcttgcc gcagccggac tctccgacca agcccagtgt cttgccttca
    13141 tcaatctcga agctgatgcc gtcaatcgcc cggacttcgc cttccggagt gaagaaagac
    13201 gtgtgcagat ctttaacttt aattagctcg gccatttatt cgggctcgcg caggcggatt
    13261 aagctatgaa agcgtaattg cgtccaaaca acatatcatc taaaccgcgc acggcgaaga
    13321 gtgcggcaac gacttgctcc agccggcaaa ataagcggat attacttggg ctggagccat
    13381 tgtcaagtta aggcatgaga ctagcgagac tgactgaatt ttacgcgctc acgtttaagc
    13441 acaaatctgc tcggacagaa ctgttacccg ataatggagt ttctcagaac actcgcgggc
    13501 caaggcaatt cttgacgaac agccgcgcgc agtctcgttc tcatctttag agccatcacc
    13561 atgccgctgc acgcttgtct atttggcagg gtaggtcttc gccagctgat cgataaaacc
    13621 gctttcgtcg agtcgccgca ggattcggtc gtcgatcata tcttctactt tgaccttatc
    13681 gagagcagga ttacggattt tcatgagccg ttgaatgttg cgcagccctt cgactgtcgg
    13741 atagggtttt cgttccacgc cgttgataat atcttgatag ccttcttcgg ctaccgacgg
    13801 atccgccagt ttaagacgct tggtaatcgt cgcgagaacc gcattcttgt ttttcggcgc
    13861 aagacaaaat gcgatgcttt ccaaaagtcc cttcatgacg ctttccatgg tttcgcgttg
    13921 ctcctgtaga aaagccccgc gagccacgag tccctgagag gaaaacggaa tattttcttt
    13981 ttgcagctcg gcgagaatcg taaagccctt ctgcttcaat cgccggctaa aaactccatc
    14041 cagcaccgtc acatcgattt gaccgccttc gagagcctgg gttgccacgg tttgatcgcc
    14101 aatgacgaga aaagtgatgt tgtccctttt ggcttctaag cccaagtgct ccagtccaag
    14161 catggcacct agccagacag ttcccgccag cgtctgcact ccgaagcgtt tgccgcgcag
    14221 atctatgggc gttttgatgt tcggggccgc gaccatgtcg tagcccaaac gattagtgag
    14281 ggacccgatg atctttaaat cggcgccgcc ggccaccgcg gtcagcacgg ttgtcccgcc
    14341 ggtgtaggcc gcgtgcacgt ctcccgccgt tagagctgcg acgaggaccg gggcctggcg
    14401 gatgaaaatg gcgctggccg aaacaccgtg cttggcaaaa aaccctacgt cttcggcaac
    14461 ccagagaggc gccaccctgg cattcatcgc cgcgtggccg atcacgaatt tgcgcggcgc
    14521 cgatgcggct tgcaatgtaa cgcaggcgag ggcgataaaa aagtttatcc cgacgaagac
    14581 aagcatgatg cgcattgatt ttctcatgca aggtttccct ttatacggtt gcgccgcgcg
    14641 tctacaattc tcggcggcgc tatccgtatt gattcttgtc ttgttagctt ttaacagcct
    14701 aacgtctgtg ccttccgctt ccctacttga cgaactgctc cttgggaaag ttcgcggtcg
    14761 cgacaacgcc accgtccacg actaacggat ggccatagat aaaatcagaa tcctcgctga
    14821 ccagaaacag cgcagccttg gcttgatcct ccgccgtccc caaccggccc gcgagattct
    14881 gaaacgttct gcttccggca acgtcgtcgt catagcctac cggcgaccct gaacggtttg
    14941 gcaccacgca attgacccga atcctgtatg gcgaaagctg caccgccatc gcgcgagtca
    15001 ggttgatgac gccgcccttg gccgccgcat aagcgatgcc gtcggtccga ccgagcatgc
    15061 cggaagtcga gccgaagttg acgatctttc cgccgcgccc ctgtttggcc atttgcgcgg
    15121 caatgtgctt cgtcacgaga tagggccctt tgagactcac cgcaatcact ttatcgaact
    15181 cgtcctcggt gctggtgaag atatcggcat ggtccgtgag cgcggcattg ttaatcagga
    15241 tgtcgatgcc gccaaaacgc gcgacaactt tttgaaccat ctgttgcaca tcactggaat
    15301 tggcgacatc gcacaataca agcagggctt catggccgct gttttcgagt tcctggacca
    15361 ccgcctggcc gcggccttcg tgcatctcta tcaccgcgat cctcgctccc tcatcagcaa
    15421 ataatttggc gatagctttg ccgatatttc ttccggcgcc ggtaatcacc gccacttggt
    15481 tctgcaattt cattcaattc tccttctttc gacctttcga cagacctcta gcgtcgctga
    15541 acaatctcga acccgtcgat ctcgtgttca gattttgaac tcgtcggggg cgctggctgt
    15601 tgcgatatcg aaagtattga gcgtgcacgc gagcatcgcg tagtaaccga cggtggcagt
    15661 gagctcgacc aggccgcgct cgccgagtcg agcatacagc gcttgaaagg tagtttcgtt
    15721 gacccgatgc gcgcgcaaca cttcgcggac gaaactgaca atctgcgcat cttcgaaggt
    15781 aaaatcctcg gcatctttgc cttggtgaac ggcgcggatg atatccaggg aaatacctgc
    15841 cttttgcgca tgatttacat gagcggccca ttcatgcttg cattgaagct cccgcgccga
    15901 taccagcgcg gccaactcta tgatcttcgg ctccaaactc gattcgaagc gcacgtaact
    15961 gcccagatgc gcagttcgag cggcaagctc cgggctgtgc agaagcgcca gaaagggtcc
    16021 gccgacgacc ccgcgacttt gcgcaattga gtcgtagatc gattggtctc ccggggccag
    16081 atcttctttc ttctctatgg cagcgattct agccatgttc cctcctcaag ccagtttgtc
    16141 ctgcagccaa gtggtcacga tgcgcacgac gtcgtcattg gattggcctt tttttcggcc
    16201 catgtgcccg gcatcggcaa tgatccgaac atcctttggg ctgccgtgct ccaacagcag
    16261 ataaatatca tccaccggat gctggtcgtc ctctttgccg ttgatacaaa gtaacggcgc
    16321 gcagggctga tccaacagtc cttgcgtttt tagcgacaga gtaggcgcga ttttcagaac
    16381 ctcttcgagc gatttcacac caaaaatgta cgagcgagcg tccaacaaac tcgccgggcc
    16441 catgagatac tgcgacgccg tcaatgtcaa agcagggcgc aaccattctt cctggaacgt
    16501 ccggtgaacg cctgctcccc aattgacggc gccccgcaat cttgtcgctt cggtatgcgc
    16561 gagcttcgcc gcccagtaac cgccgaagct tccgcccatc acggcgaccc gtttgccgtc
    16621 gatttcgtca cgcgtctcga gatagtcgat ggccgcggag aacgtgcgct ctgccctcgg
    16681 atcgttagcc ttacaagggt tctcgccggt accgggcata tccatggtca aacaggccag
    16741 ccccttcttg agcaaaatgt cattgttggt tcggcgatcc tccttccagc catcgacgcc
    16801 gccccaatgc agcactaccg gcgggcggtc gagttctttg gggatctgca ggtaaccgac
    16861 gaccttttta ccttcgaaag gaatttcgat tcggcgcagc ggcgggttca tggttggcgc
    16921 ggcttttaga aaagatcgca gcgcgcctct ataacaagcc atcttttgcg ggctgctcgg
    16981 aactggataa cggccgatac gataatattc gtaagccaag tagtacgttt caccggcttg
    17041 cgttgatcgg ccggttgtct cctgttcgtc tgcaagagct tcatatttag ctcccagctt
    17101 tccccattcg ctgccccaca gatcagggtc gaggctattc aagttggcga gcacctgctt
    17161 tacatcctcg cgcgcgaccc gttcgaacgg attgatccgc ccggcgcggt gcaaaatatc
    17221 ggctttgatt tcttcgagcg atttttccgc catgatctac ttctccacat tcggcccgtc
    17281 tggcgaatag tacgccaacg acgaagagca cgaaggacgc gaaggttagt taactttaca
    17341 cctgaagtta atgctctcgt gcgtacgttg caatgttctg tgcggctttc agccagccct
    17401 ataattatga tttagtcccg ggctcgttag ccaatgcttt gagcgccgct ttgaaaaacc
    17461 tatcgtcgat aaactgttcg ggtttgaaat ttggtttttc cttgagcagc acgaactcca
    17521 gttccgcgcg cttgtataga ccctcggggg tgagcgcggg aatgtaggcg tcacgcataa
    17581 gatcgtaaag cgacgcggcg atatcgcgct cgttgagccg gccattcttc atgatgatat
    17641 cgatgctgcc ctcgcggttg gctcgaatcc agtaaagcgc cttcatcacc gctttgacca
    17701 ttcgttccgt catctccggc tccgactgaa cgtgcttggc cattgcctgc agggcgctga
    17761 acggcacgtt cttgaactcg tcgccgagat agaccaaacg cttcatgccg gccttgagaa
    17821 gcggccggtc tgtgggtggc gccatcaacg cagccgaggc gcggccctgc atcaatgcga
    17881 tcgccttgcc ttcgttgtcg ggaatattga ggtactcggc aaatttgtcg ctgaaaccat
    17941 acttcgcgaa cagcgcctgc aagacgctat aggtcgagcc gccgaaggtt accgcgacct
    18001 tcatgccttt caaatcatcc agtcgattga tctccggacg cacgatcaaa ccgaaaggaa
    18061 tttgattgaa aatctgcagg agcacgcgaa cgggttcgcc gcgaaacatc gcctgcatcg
    18121 agggaccgaa ggcggacgaa tagtgcacgc ttccggacat caacgcctga acgttcatcg
    18181 cattgccgcg cagcacgacg atgttcacgt tcgtaagacc catctcttca taaaattttt
    18241 tttctttggc aacgatgtaa gccaaatgct gcacattgcc gggaccgggc agggaaacat
    18301 agacgggttt gctgatctgc gcctgcagcg ggagggaaaa aagcacgata ccgaaggtca
    18361 aagcgaagat atccgatgat ttcatcgaca acctcccgtg gctaagcttg gaccggctta
    18421 acagaaacgc cgcgcttatc gcaacccctg gagtctggga tcaagaactt cgcgcaaccc
    18481 ttccccgacc agatagtagg cgagcacagt gatgaaaatg gcgaagcccg gaaacaccgc
    18541 cagccaccag gcaaaggtgg tggtgctggc ttcctgtaaa atcgaacccc acgtgaccaa
    18601 ctcggccggc acaccgatac cgaggaagct aagactcgac tcgaccagaa tcgcaccggc
    18661 gataccgagc accaccgaga caatgacggg ggctaacgcg ttcggcagga tatgcttgaa
    18721 cattacgttt cggtcagtca ccccgagcgc gatcgccgat gtcacgtaat cctgatttct
    18781 gattctgaga aactcattgc gagtaaagcg cgcgatacca acccaactcg ttgctccgat
    18841 gatcgccatc gtcaggaaga tgctcggggg gagcagcgct gcgacagtga tcagaaggaa
    18901 gaatgtcggg atcgacagca tgatctcgaa taaacgtgaa atgattaagt ccacccaccc
    18961 ggcgaagtaa cctgcgatgg cgccaagaac gaggccgatg atcaccgcga tcccgaccgc
    19021 gacaaaacca atagaaagag atattcgcgc accatggatc attcctgaca tgacatcgcg
    19081 cccgagctta tcagtgccga gccaatgctt tcccgacggc ggctccagcg gctcgagtaa
    19141 attgatgttc gttggccgat atggaaccgg cggaaacagc gcctgagcgc cctcaacttt
    19201 cttgaaatcg acgttcagca attccggtgg ccaatggcca acgccagcgg cgaccatgta
    19261 gctgcggaga atcggaaagt aagccttgcc tccgtaacgg aagtaatagg gtttgtcgtt
    19321 agcgaggaaa tcagcggata acgccatgca agccaggatg caaactacgt aaaatcccgt
    19381 gagggcgatc gagttgcgct tgaaacgttt ccaaacgtcg cgccacaacc ccaccgacgt
    19441 ggcggactcg gcgctcccga tttcttctgg agctgcttga ttcgattgca gcgcgctcat
    19501 tgttgccgtc gaccaaagac gatgcgcggg tctactgctg caagaagcag atcggagatc
    19561 aggatgccgg tcagggtgag aagcgcgctc accgtaaaga gcgccagcac tgtgggatag
    19621 tctcgcgcta aaaccgattc aaacccaagc tgtccgagcc cgggaattga aaagatggtt
    19681 tcaataatga cgctgccgcc gatcagcccc ggcaggatgg atgctaatat cgtgacctgc
    19741 ggaatcaagg aattgcgcaa tacatgcttg agaatgaccc gcttctccgg aagtcctttg
    19801 gcgcgggcga cctggacata atcctgcgcg aggacttcca gcatgctcgc ccgcatataa
    19861 cgcgataatc cggcaaagcc atcgtacgag agcagaacaa ccggcaagaa gatgtgccag
    19921 gcgcgatctt ggagctttcg ccagataggc cagttatagg agaaatcaat cgactccagg
    19981 ccgccgggcg ggaacaaata gtagaagtca ccgccgcaaa taaaaacgat ggccatggtc
    20041 gcggcccata ccaacggcac cgcaaacaga acaaaagccg ccaaggtcgt gacctggtcg
    20101 ccaaaggaat ttggatgcgt cgccgaatag acgcccaacg gaatggcgac tagataaacc
    20161 accaataggg accagaaatt taatttaact gtgaccggca ggcgctcggc gatcttcgac
    20221 cagaccgact gattgtcttt aaacgattcg ccgaaattga gcgtgccgat attcttaagc
    20281 cacaccaagt attggacgtg aagcggttta tcgagtccgt actgcgcgcg ccattgcgca
    20341 atggcttcgt cgaccgagcc acgtttctcg accgatattc cagcccccgc tgccgcgccc
    20401 agaccgcctg ccgcgcgcag ttccgccgga tctccggggg ccagacgcat gatcagaaat
    20461 ccgatgaggg taatgccgat aaaggtaggt accatcagca acaggcgctt tatcagatag
    20521 ccggtcattg cggagcgatg ctgtttgcgt atttttgttg cgccaatggc acccaccaat
    20581 cgatgggctt gagcccgtcg ggaaacactt cgacaccttg gaagcgacga tgcaccgccg
    20641 aaacggtctt gcggacatga agaaaagtgt aaggctgttc gtcatgcagg atttgttgaa
    20701 attctttata gagctcgatg cgcttttgcg gatcaaactc ctgacgatag tgttctagaa
    20761 tctcatccac gcgcgggttt ttgtagctga tcgcgttcga gcctttgttg gcggcctgag
    20821 aggagtgcca gacctggtag gcatcgggtt cggccgtgga catcgcccaa cccagcacta
    20881 ccgcgtcaaa ctgatgattc ttgacgtcgt tgagaaatat ggtccagtcc aactgacgga
    20941 cgctggcggc gatgccgtgc ttgttgagtt cgtccatgag caccaacgcg acactttccc
    21001 gcaccgtatt cccggcgttg attttaattt caaaacgcaa gggaattctg ttgccgtcga
    21061 taaccttgtc aagcacgcca tcaccgtcag tgtctttcca gccggcttca t
//
LOCUS       DASUVS010000457         4187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1077855, whole genome shotgun sequence.
ACCESSION   DASUVS010000457 DASUVS010000000
VERSION     DASUVS010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077855"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VF182_24085"
     CDS             <1..233
                     /locus_tag="VF182_24085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440218.1"
                     /translation="VSDGHINLAILPARNRREGIYHFGIAVDDVKGAVETALAAGATG
                     GTAELPKDGRFAETFVLDPVGTRVDLSPGWKV"
     gene            509..1447
                     /locus_tag="VF182_24090"
     CDS             509..1447
                     /locus_tag="VF182_24090"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6440219.1"
                     /translation="MSDFYSAWLERSKSNQKKVDEAPRVAKSKDLKWVRTPQDYKAAL
                     MIAPETGFPTGGSCLMRAEIPVGWHTGKHFHGEEAIYVESGEGFLILDEKRYDFFPGS
                     ILHIPYRSPHQLFNTGKAPVGYLSGLAWHLEADVYMGKLEQLEECGENDPKILANFPK
                     EESQYWPEDGRRISMHQQQHEKSGESKHGATYFLMGRSGKQNGFRATGVAISSIFVDL
                     PHTKSHSHAHPEAYLYALEGDGYSEIGGKRYDWNQGDAVHVPPGMLHHQHFNPFDRDM
                     KELRFEFGVRYWFVDQWKKGYTTVDKHLKATTHMDD"
     gene            1793..2059
                     /locus_tag="VF182_24095"
     CDS             1793..2059
                     /locus_tag="VF182_24095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440220.1"
                     /translation="MTLRNPRNGAPKMMLGFIVRRCAADIGHTPSPDEFAAWANARRQ
                     DGHEYCLFGKPISPSIAKVMFQQPGRLVTVNGDTFEKHWRSPRG"
     gene            2363..3349
                     /locus_tag="VF182_24100"
     CDS             2363..3349
                     /locus_tag="VF182_24100"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440221.1"
                     /translation="MAASIVGVLSAFQAHAQKKPEKLRIGYAARAVAHSVPFVAKEAG
                     FFSEEGIDAEVVRTSGSIAPMALIANEVDLAIMSAYLMIPVSERNRDIVMLGGFSRYA
                     SMVLVARPEIKSAQDLKGKVVGIQRPGDAYEKSLRFGLRHLGLDADKDVTMLALGTND
                     VMWGALQTGRVAATILSPPGTLFARKAGMNFMVDLTDLKLEYQGSTIATRRSLVQKNP
                     ELATRALRAIVKGVHLFKTRKEDTLRILGKFLGTHDREALEESWEYAAKMPAKPYAVE
                     SAVQAVLDHLAEGQPKFAKYRPADFIDGRVLSEIDKSGYIDKLYGAQESKGR"
     gene            3382..>4187
                     /locus_tag="VF182_24105"
     CDS             3382..>4187
                     /locus_tag="VF182_24105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX6440222.1"
                     /translation="MLRDRQPGQFRIISGLCAQGDQPAAIEKLYSGIEQGKRHQVLLG
                     VTGSGKTFTIANVIEKVNKPTLVLAPNKTLAAQLYNEFKELFPDNAVRYFVSYYDYYQ
                     PEAYVPSTDTFIEKDSAINDEIDKLRHSATKALLERRDTLIVASVSCIYGLGSPEAYF
                     ELMVYLEEGARVERDQMLRKLVDIQYQRSDFDFHRGTFRVRGDCVEVFPAYEDKSALR
                     IEFFDDTVEGIYEIDPLRGKVQRRVDKAMVYPASHYATTESRLKQATRDIR"
BASE COUNT          998 a         1098 c         1165 g          926 t
ORIGIN      
        1 atgtatcaga cggccacatc aatctcgcca ttctgccggc gcgcaatcgc cgcgagggca
       61 tctatcattt cggcatcgca gtggacgatg tgaagggtgc ggtcgaaacc gccctggcgg
      121 cgggggctac aggcggcacc gccgagcttc caaaggatgg gcggttcgct gagacgtttg
      181 tgctcgaccc ggtcggcacc cgcgtcgatc tgtcgcccgg ctggaaagtg tagacgaaaa
      241 tccggtgata ctaagtacaa gtggcgtcag acatagggta ttatgcggct gaaaccgctc
      301 atcgtttcgt cgatcggcgt cgatactgac aactcttagt catcagccaa ctctcggact
      361 cctcgctctc tgtttcgcgg tcaatctctt gcgcgctcgc gcgtcagcgc tgcggcagtt
      421 catatcggcc gtcgcatgca atttgacaaa aaagtacccc ttcgttagtt agataaggat
      481 tacagacgag ttttgagcag gaggaacaat gagcgatttt tacagcgcat ggttagaacg
      541 gtccaaaagc aatcagaaga aggttgatga agcgccccgc gtcgcgaaat ccaaagacct
      601 aaaatgggtg cgcaccccgc aggactataa ggctgccttg atgattgctc cggagaccgg
      661 atttccaacc ggcggcagct gtctcatgcg agcggagatt ccggtaggct ggcatacggg
      721 gaagcacttt catggcgagg aagcaatcta tgtcgagagc ggcgaaggct ttctgattct
      781 ggacgaaaaa cgttacgact tctttcctgg aagtattttg cacattccat atcgttcacc
      841 tcatcagctc ttcaatacgg gaaaggcgcc cgtcggttac ctttcagggc tagcttggca
      901 cctggaagcc gacgtttaca tgggcaagtt ggagcaactc gaagaatgcg gcgagaacga
      961 tcctaagatt ttagctaact ttcctaaaga ggagtcgcag tactggcctg aagacggccg
     1021 gcgcatttca atgcaccaac aacaacacga gaagtcgggc gaatcaaagc acggcgcgac
     1081 ttattttttg atggggcgga gcggtaagca aaatggattc cgggcgaccg gcgtagcgat
     1141 tagctcgatc tttgtcgatc taccgcacac caagagtcac agccatgctc atccggaagc
     1201 ctacctgtat gcgctggaag gggacggcta ttccgagatc ggcggcaaac gttacgattg
     1261 gaatcagggc gacgccgtcc atgtgcctcc aggcatgttg catcatcaac atttcaatcc
     1321 cttcgaccga gatatgaaag agctccgctt cgagttcggc gttcgttact ggttcgtcga
     1381 tcaatggaag aaaggctaca ccacggtgga caaacacttg aaagcgacta cgcacatgga
     1441 cgactgacga taatcaaatg acagcactta acggcgggtt aacggaaatg ttgcccgccg
     1501 ttttgcttta acggaaatag aacggcacat ctttagatat gatttgcgct cacctggatg
     1561 cgcagttgaa cgggaagcag ggccaggagc aaaccatctg taaacgaagt cgcgggacca
     1621 ggcaaaaaaa ttagtttgaa actaaaccat ggctatcttc gatgcgcagg agtgcccctc
     1681 gaccattttc aaccgcggaa tttgctttcg gaagttatcg atcaactaaa aaattccgtg
     1741 cagggataaa actttcttgc aacaaacaac cagatctttt atagagtatt caatgacgtt
     1801 acggaatccc agaaacggcg cgccgaagat gatgctgggc ttcatcgtgc gccgttgcgc
     1861 agccgatatc ggccatacgc caagtcccga tgaattcgcg gcgtgggcca atgcgcgtcg
     1921 ccaagacgga catgagtatt gcctgtttgg caaaccgatt tcaccctcca tcgccaaagt
     1981 catgttccag cagccgggcc gtttagttac cgttaacggc gatacattcg agaaacattg
     2041 gcgcagcccg cgcggctaga tcttggacct cctgcagtta tgccaatttc gcccgcccgt
     2101 tcctgacaca actccccgga tctaaatcat taggcgctgg cggcgttgcc cgtgagtttg
     2161 acctgatcac gcagtcgaac gatttctttg agagtccatc atgatcgctg gcccattggc
     2221 atcccgcgtc acaggcccgg ctagtcgccg gatagccaaa gattgatcgg ctaaaacggc
     2281 ctgttgtcct ttgccgccta tgtcgtggcg cttttaaacc agatggaggg gattcacaat
     2341 gagaatgaaa cggatctttt tgatggctgc tagcattgtc ggggtcctat ccgctttcca
     2401 agctcacgct cagaaaaagc ccgaaaagct tcggatcggc tatgccgcgc gcgcggtggc
     2461 gcattcggtt cccttcgttg ccaaagaggc gggatttttt tccgaagaag gcatcgacgc
     2521 cgaggtggtc agaacgtcgg ggtcaatcgc gccgatggcg ctgattgcca acgaggtcga
     2581 tctcgcgatc atgtcggcct atttgatgat tccagtctcg gaacgcaatc gcgatatcgt
     2641 catgctcgga ggcttttcgc gctatgcctc aatggtgctg gtcgcccgtc ccgagatcaa
     2701 aagcgcgcag gatctaaagg gtaaggtcgt cggcatccag cgcccgggcg acgcctacga
     2761 aaagagcctg cgctttggct tgcgccattt gggtttggac gccgacaagg acgtgacgat
     2821 gctggctctc ggcaccaacg atgtcatgtg gggagcgctg cagaccggca gggtagcggc
     2881 aaccattctc tcgccgcccg gaactttgtt tgcgcgcaaa gccgggatga atttcatggt
     2941 cgatctcacc gatctcaaac tggaatacca aggttcgacg attgccacgc gccgttcgct
     3001 ggttcaaaaa aatccggagc tggctactcg ggctttgcgc gcgattgtca aaggcgttca
     3061 tctatttaag acccgcaagg aagatacttt gcgcatcctc gggaaatttc tgggcaccca
     3121 tgatcgcgag gcgctggaag aatcctggga atatgccgcc aagatgccgg ccaagcctta
     3181 tgccgtggag agcgccgttc aggccgtgct cgatcacctg gcggaaggac aacccaagtt
     3241 cgcgaagtat cgacctgctg atttcatcga tgggcgcgtc ctcagcgaaa tcgacaaaag
     3301 tggctatatc gataaactct acggcgctca agagagtaaa ggccggtagc agcggccggt
     3361 tgtaattgct attttgtttc gatgctaagg gacaggcaac caggacagtt ccgaatcatt
     3421 tcgggcttgt gcgcgcaggg cgatcaaccg gccgctatcg agaagctcta ttcgggcatt
     3481 gagcagggaa aaaggcatca ggttctatta ggggttaccg gatcgggaaa aacctttacc
     3541 atcgccaatg tcatcgagaa ggtcaacaaa ccgactctgg tgttagcgcc gaacaagaca
     3601 cttgccgccc agctgtacaa tgaattcaaa gaattgttcc ccgataatgc agtccgttat
     3661 ttcgtcagct attacgatta ttaccagccg gaggcttatg tgccttcgac ggataccttc
     3721 atcgagaagg attccgccat taacgacgag atcgacaagt tgcgccactc ggcgaccaag
     3781 gctttgctcg aacgacgcga cacgctgatt gttgccagtg tttcatgcat ctatgggctg
     3841 ggttcgccgg aggcctattt cgagttgatg gtttatctcg aagaaggcgc ccgagtagag
     3901 cgtgatcaaa tgctgcgtaa gctcgtcgac atacaatacc agcgcagcga ttttgatttc
     3961 caccgaggga ctttccgggt gcgcggcgac tgtgtcgaag tgttccccgc ttatgaagac
     4021 aagtcggcgc tacggatcga gtttttcgac gataccgtgg aaggcatcta tgaaatcgat
     4081 ccgctgcgcg gcaaagtgca acggcgggtg gataaagcga tggtgtatcc ggcgagccat
     4141 tacgcgacca cggaaagccg cttgaaacag gcgacccgcg atatccg
//
LOCUS       DASUVS010000458         4116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_204546, whole genome shotgun sequence.
ACCESSION   DASUVS010000458 DASUVS010000000
VERSION     DASUVS010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204546"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VF182_24110"
     CDS             complement(<1..864)
                     /locus_tag="VF182_24110"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6440223.1"
                     /translation="MTIRSFELLQPRSVDEAIELLGKHGDEARPIAGGTTLIILMKQR
                     ALHYPYLVDLQSIPGLSGISKENDGVRIGALATHRSVELSPLIREVFPAVAEAFSKIG
                     NVRVRQTASVGGNLAHADYRLDPPPPLLVLGSYVNAQGPNGSRSLPIKDFFRGLYETA
                     LEPGELIVDVKLPFMPPDSRAVYLRYSSLSGNDWPCLGVAALAIRQNRRCQELRLALG
                     GVAATPLLIERLDMAKDQSLDDSLIDKLLDIVDEQISPSSDMRGSEWYKRKMVRVFVK
                     KALLELDDRVSA"
     gene            1012..1824
                     /locus_tag="VF182_24115"
     CDS             1012..1824
                     /locus_tag="VF182_24115"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6440224.1"
                     /translation="MFASIRHIAVMTENWDRMAKFYQTIFDMKKITNGMTDETGTYNK
                     DRGHLSDGVIGLALLQRQPGFRSGLDHFGFAVDDVPLVRDRLKQHYPDVYVAQSQSHV
                     PFAGLRSHDPDGNQFDLSQKGMDNVREGYVEFGWEQPRWINHVCIRSGRPAHIAEYYQ
                     KVFDLRPVEGMSGDGSYYLSDGKVSLAIRPWDMVAYRGLMAGLDHFGFKVDDLSKTKK
                     DLEALDGVEPKSGARKIAIGRDGEVRQKNLEGCKLCKHAISDPDGVLLDLTD"
     gene            1990..3117
                     /locus_tag="VF182_24120"
     CDS             1990..3117
                     /locus_tag="VF182_24120"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX6440225.1"
                     /translation="MATSLKQLIDSYDPNLLLSEASTPPRSWYSDPRILDLEQRTVFS
                     HSWYVIGRTEQLRKTGDYITCEVAGEPILLVIGPDGVLRSFYNVCRHHAAAVMTERQG
                     RAEHLRCPYHGWTYNLEGALVIAPEFGGVCNFNKSGNGLAPVQTAIWQGWVFVKLVSN
                     GPALEEFLGEDLVEQIRRLKLEQLSWFERRSYTVQCNWKVFIDNYLDGGYHVPHIHGD
                     LNSVLDYSAYKIATGERFCVQSSPIITGKADVQTLETRQGQALYYWIYPNFMVNWYEG
                     VMDTNLVVPRGIDRTEVIFDYYFTDVSEQGREKNLASIAVSERIQDEDVGICESVQRG
                     LSSRSYSTGRLSVRREAGEHLFHRLLHADLKAGLANDARLI"
     gene            3432..>4116
                     /gene="argC"
                     /locus_tag="VF182_24125"
     CDS             3432..>4116
                     /gene="argC"
                     /locus_tag="VF182_24125"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX6440226.1"
                     /translation="MPHRIYIDGQAGTTALRIRDWLTGRDDLEVKFLPEELRKDPIAR
                     KKALRDAEIALLCLPDDAAKEATAWLADSPVRILDASTAHRIAEGWVYGLPELTSGQR
                     ERIAQAQRVANPGCYSSAVILLARPLVDAGLIAADAAFSVHALSGYSGGGRSMIERWE
                     NPTGPLKNLPYEAPYSVGKLHKHIPEIMRWTGLTNEPQFIPAVGPFRCGMRVQVMIHA
                     KALPEAGAGS"
BASE COUNT          962 a         1132 c         1129 g          893 t
ORIGIN      
        1 cgctgaaacg cgatcatcca attctaggag cgcctttttg acaaacacac gaaccatctt
       61 gcgcttgtac cactcggagc cgcgcatgtc ggaagaaggc gatatttgct catcgacaat
      121 gtcgagcaac ttgtcaatga ggctgtcatc gagtgactga tcttttgcca tatccaatct
      181 ttcgatgagc aggggtgtcg cagcaacgcc gcctaacgcc agcctgagct cctgacagcg
      241 gcggttttgc cggattgcca acgccgcaac gcccaagcac ggccaatcgt tgccggatag
      301 cgaactgtac ctgagataaa ccgctctgct atctggcggc atgaaaggaa gtttcacatc
      361 gacgataagc tcgccgggtt ccaatgcggt ctcgtaaaga ccgcggaaaa aatctttgat
      421 aggcagactt ctcgagccgt taggaccctg ggcattaacg taactcccga gaaccaacag
      481 cggcggcggt ggatcgagac gataatcggc atgagcgaga ttgccgccca ccgaagcggt
      541 ttgcctaacc cgaacattac ctattttgct gaacgcctcg gcgaccgccg ggaacacttc
      601 gcgaatcagc ggcgagagct cgacactgcg atgagtcgcc agcgcgccga ttctgacgcc
      661 gtcattctcc ttagatattc cacttaaccc cgggatcgat tgcaaatcca cgagataggg
      721 atagtgcagc gctcgctgct tcatcaatat gatcaaggtt gtgccgccag cgatggggcg
      781 ggcctcatcg ccgtgcttgc ccagcagttc aatggcttca tcaaccgagc gcggttgcaa
      841 taattcaaat gaacggatcg tcattaggat ttctcaatta cagtgatgtt tctagccttg
      901 gcgattccga taagtcaaga acacggcatg attcaccgca gaaggaatcc ggaagggttg
      961 acaggcccag tcaggtttga gagaactcgc tcaattagga gggccgtaat catgtttgca
     1021 agcatcaggc acattgctgt catgactgag aattgggacc gcatggcgaa attctaccag
     1081 acgattttcg acatgaagaa aatcaccaac ggcatgaccg acgaaaccgg cacttacaat
     1141 aaagatcggg gtcatctgag cgacggcgtt atcggcttgg ccctgttgca gcgccagccc
     1201 ggattccgat ccggcctcga tcatttcggc ttcgccgtcg atgacgtgcc attggtgcgc
     1261 gaccgtctaa agcagcatta tccggatgtc tatgtcgcgc aaagccagag ccacgtaccc
     1321 ttcgccggct tgcggagtca tgatcccgac ggcaatcaat ttgatctgtc gcaaaaagga
     1381 atggacaacg ttcgggaagg ctatgtcgaa ttcggttggg aacaaccgcg ctggatcaat
     1441 catgtgtgca tccgcagcgg ccgtccagct catattgccg agtactacca aaaggttttc
     1501 gacttgaggc cggtggaagg catgtccggc gatggcagct attatctgag cgatggcaag
     1561 gtgagcttgg cgattcgtcc gtgggacatg gtggcctatc gtgggttgat ggccggcctg
     1621 gatcattttg gtttcaaggt tgacgacctg agcaaaacta aaaaagatct tgaagcgctc
     1681 gacggtgtcg agcccaagtc cggcgcacgg aaaatcgcca tcggccgcga cggcgaggtg
     1741 cgccagaaaa atttggaagg ctgcaagctg tgcaagcatg ccatctcgga tcccgatgga
     1801 gtactgctgg atttgacgga ctgagttcat caagccggtg gatacatcca aggagctagc
     1861 cgaccccaaa gctgcgctgc attgagtcgc tataccgatg ccgcctggtg ccgtcataga
     1921 ccgaaggatc gcgatctaag attgtaacca tacaatgact cgccccgcgg gacatacttg
     1981 ctattcgctg tggcaacttc cttaaagcag ctcatcgata gctacgatcc caatctactg
     2041 ctgagcgaag cctccacgcc tcctcgttcc tggtactcag atccgcgtat tctcgaccta
     2101 gagcaacgaa cagtgttttc acactcatgg tacgtaatcg gccgcacgga gcaacttcgc
     2161 aaaacgggcg attacattac ctgcgaggtt gccggcgaac cgattttgct cgtcataggg
     2221 ccagacggcg ttctgcgaag cttctacaat gtctgccgtc atcatgccgc cgctgtaatg
     2281 acagagcgtc aaggccgggc cgagcatctt cgctgtccgt atcatggctg gacttataac
     2341 ctggagggcg cgctcgtcat cgcgccggaa ttcggtggcg tatgcaattt caataaatca
     2401 ggcaatgggc tggcgcccgt tcaaacggca atctggcaag gctgggtctt cgtaaaactc
     2461 gtctcaaacg gtccggcact tgaagagttc ctcggcgaag atcttgtcga acaaattcga
     2521 agattgaagc ttgagcaact ttcttggttc gaacgccgct cctatacggt ccaatgcaat
     2581 tggaaagttt ttatcgacaa ctatctcgat ggcggatatc atgtgccgca tattcacggc
     2641 gatttgaaca gcgttctcga ttacagcgca tacaagatcg caaccggcga acgcttctgc
     2701 gttcaatcga gtccgataat caccgggaag gccgatgttc agacattaga aacgcgccag
     2761 ggacaagctc tctactactg gatctatccc aacttcatgg tcaattggta tgaaggcgtg
     2821 atggacacga acctggtcgt gccacgcggc atcgaccgga ccgaagtcat tttcgattac
     2881 tacttcaccg atgtttccga acaaggccga gagaagaatc ttgccagcat cgccgtcagc
     2941 gagcgaatcc aagacgaaga cgtggggatc tgcgaatccg tgcagcgcgg cctttcatcg
     3001 cgctcgtatt caaccggacg gctatcggtc cggcgcgaag cgggcgaaca tctttttcac
     3061 aggctcttgc acgccgatct gaaagcagga ttggccaacg atgctagact aatctgaaaa
     3121 acagctatca agaaaagttg gttatccgtt aacgcgcacc gcgggcaagg tgaagtcaca
     3181 actggttcgt gggttcagga gttcatcagc aagcagccct tttgtattct gacaaccagc
     3241 aaagccaacc gcgcccgcga cgccgcgccc aaacgcggca cgccggtttg tcaaagtgct
     3301 caacgacaag caactttttt attcccgggg tgaagtagcg gcaaagcttg ctggtgcacg
     3361 atccattttg gcacttttgc taagcctgag gattcgtcgt aaacttgttc cgtttgatga
     3421 aggactttgc aatgccgcac cgcatatata ttgatggcca agccggaacg actgcgctac
     3481 ggattcgcga ttggcttacc ggccgcgatg atcttgaggt caagtttctt cccgaagaac
     3541 tgcgcaagga tccaattgca cgcaagaagg cgcttcgaga cgccgagatc gcgctgcttt
     3601 gtctccccga tgacgctgcc aaggaagcta cagcttggct ggccgactcc ccggtaagaa
     3661 tactcgacgc cagcacggcg catcgcatag cggagggttg ggtttacggc ttgccggaat
     3721 tgacgagcgg ccagcgcgaa cgcattgcgc aggcccagcg cgtcgccaat cccggttgtt
     3781 actcctcggc ggtgatctta ctggcgcgtc cgctggtcga tgccggcctg atagctgccg
     3841 atgcggcatt ttcggttcat gcgctttcgg gttattcggg cggcggtcgc tcgatgatcg
     3901 agcgctggga gaacccgact ggaccgttga aaaacctgcc ctacgaagcg ccttacagcg
     3961 tcggcaaact tcacaagcac atacccgaga tcatgcgttg gaccgggctc accaatgaac
     4021 cgcagttcat cccagcagtg ggaccttttc ggtgcggcat gcgggtgcag gtgatgatcc
     4081 acgccaaagc cctgcccgaa gccggcgccg gctcgg
//
LOCUS       DASUVS010000459         9315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1517925, whole genome shotgun sequence.
ACCESSION   DASUVS010000459 DASUVS010000000
VERSION     DASUVS010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9315
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517925"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VF182_24130"
     CDS             <1..622
                     /locus_tag="VF182_24130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440227.1"
                     /translation="NMISLPWEYADQTLYAAYEKTKAQGRSGFENFHELRASIAAGKP
                     KQQEHPIYRRLDPDSVTGGSWRERSRFLLEEPELHFWFLDEDWVTPYLSQLDEAKSSR
                     LVLNQVQKEERLAGIVRDAVKAFCAGEIGAVLQRRMEDMALYFLETGRDDAARLSLAV
                     AIQIGTGDPGPLDISFLTGLVQKSFGYYLSQQKNKEAEEPSFIVKP"
     gene            complement(629..1348)
                     /locus_tag="VF182_24135"
     CDS             complement(629..1348)
                     /locus_tag="VF182_24135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010887116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX6440228.1"
                     /translation="MAGKEMKFELDKLTEDSWRMFRIMGEFALGFDRMNQIGSPAVTV
                     FGSARTRITSRYYDQARKLGRALVEAGYAVVTGGGPGTMEAANRGACEAHGTSVGLNI
                     LLEHEQKPNPYQTISLQFEYFYVRKVMLAKYSAGFVVFPGGFGTLDEMAEILTLVQTQ
                     KIRPFPIYLVGSSYWSGLIQWFRNTLAKEEAIAKGDLDLFKVLDDVTEIPSDIRRYHT
                     SARSAGFKVPREKDRKLAVGR"
     gene            1607..4123
                     /locus_tag="VF182_24140"
     CDS             1607..4123
                     /locus_tag="VF182_24140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX6440229.1"
                     /translation="METSRRLLRAVKWLIDRLAAYFSPAFDSTVEVRLRVFSALKGLA
                     ALAALGLIMLCLYALVLIPFTPSIADLQKAKIDQPSMLISSDGKRIATYKPMNREWVR
                     LNRVSPHVINALIATEDHRFYQHHGVDVRRTLSGVFRTVLGDTEGGSTLTQQLARNLY
                     PEEIGRERTITRKIKETITAIKIEYAYTKKEILETYLNSMPFLYNAFGIEMAARTYFD
                     KSAQKLDVIESATLIAMLKGTSYYNPVLNPERALRRRNVVLSQMVKRGVLSRAEFERL
                     KTRPIRLDFERQPEAIGIAPHLAAHIRKWLIDWADRNDHNIYADGLRVHTTIDSRLQT
                     IAKHAVSRQAEALQAVADVEWGSSSPRLISTSAKAYVDMRRRVQPFAYFWKSKSGLLD
                     VFIRESAAYRAAIQSGAEPGAALALLQSNSEFMSKLRAAKTRLQAGFVAIDPATGEIK
                     AWVGSRDFKTDQYDHVAQARRQPGSTFKAFVYGAALEQGMSPNKRFTDSPVEIRLPDG
                     TIWRPSDLGEPSGRRMTAREGLIYSKNTITVQVMQEIGAKKTAELARKMGINRSKLDE
                     VPALALGTSPVTPLEMVSAYSTIAASGVYRQPTFITRITDNKGKVLAEFATQDKRAMT
                     EKTVGDLINMLRGAVDQGTGQAVRTQFGLRGDFAGKTGTTQNNSDGWFILMHPRLVAG
                     SWVGFNDPRVTMRSNYWGEGGHNALLLVGDFFQQTLASRLIDSRADFPFDRYYESIWD
                     PYFDMAKEWLGGMLRGWFGGEKPKPAPLPRTSPRRDLRFEAPGSEQSQNQKEPMVEQF
                     GGTRDRGEQRQLEREAEKDRLLERWRQKQQQREQETRVTD"
     gene            4270..4695
                     /locus_tag="VF182_24145"
     CDS             4270..4695
                     /locus_tag="VF182_24145"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6440230.1"
                     /translation="MAVEIIDMPRLAHEQKRRKVVFNTPRFHAWVHYYPNPGERDDMH
                     CHNADQTFYVIEGECTMHFPDGGKAIMKPGMAAMITGGSFYQLENSGDGAMIMMGNRS
                     GPSEAIQHINYELRKDIKKLSKEELERANIPYLSMQVRG"
     gene            5026..6021
                     /locus_tag="VF182_24150"
     CDS             5026..6021
                     /locus_tag="VF182_24150"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440231.1"
                     /translation="MRRALLGAFIGLMLLGAVTLQYAYAAQPLTKIVMTTGSFSEREA
                     AMYVAQDQGIFRRYGLDLSFVHVRNGPVGMAALAGGDTQLHEGSATGAVLGSAAEGTD
                     LVFVAGLINKLIGNIMASPKIKAPSDLKGKVIGVTSASGGSWMFTTLALEYWGLDAKR
                     DGINFRILGDESVRSQALINDSIAATHVGYTFAAPLKSRGFTNLADLAQLPIPFQSTG
                     VLTKRSFIKASPEVVESVLRSVVDAMAFIAQPENKQAVLKSIAKGLRLATAEQAIEGY
                     ESLPLLYDRRIYPTVDGIRNVIRLLGQTNEKIRRLKAEDLVDDRFVKKLEKEGRF"
     gene            complement(6167..8002)
                     /locus_tag="VF182_24155"
     CDS             complement(6167..8002)
                     /locus_tag="VF182_24155"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6440232.1"
                     /translation="MIYEPLEGFKSKALQDVLRAIRYFRDDLHLILLLLFLTACSTAL
                     GLLQAWPLAVLVDSALGTPTMDSWMHRLFLAPLPDDPISRIVGLGIMALVLRFAHELI
                     AMARRLLAPRIHYNGLLRVRGDLYRKLQGMHLDYHRSRPMSDSLFRLTTDTLGFQAVL
                     TVMINLLIAGITLCVIAGLLIGRNAALTLIALSIAPPLMWVNVIFGRRLSEKTRKSRE
                     SDSAFTTSIQRSMSAIVLTQAFGREEDEYHRFGSSARRCVRAWVGIHRQEVAYGLSVG
                     VILAVGASLILTYGGMLLHRHQLTPGELMIFMTYLGMIYDPLCQMSGFRFNLESGLAG
                     ARRVFEVLDRDPVVTDSAEATALSLQPRSLILEAVGFEYSADQKILQRISVNIEPGQS
                     VAFVGSSGAGKTTLLNLLLRFYDPTVGRVKLDHYDLRQIKLKDIRRHIALVLQDSVIL
                     PTTIAENIAYGCPAATYEQMREAARLAGATNFIEALPDQYQTALLDSGMNLSGGQRQR
                     IALARALLTKAPILVLDEPTSALDSEHEQIVIDTLNSLKTKHTVVLVSHHIGAVMNCD
                     CIYVLDQGMIVEHGSHQELLGQGGIYAAMAKQQLNPGFPVFPEAA"
     gene            complement(8442..9020)
                     /gene="pal"
                     /locus_tag="VF182_24160"
     CDS             complement(8442..9020)
                     /gene="pal"
                     /locus_tag="VF182_24160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX6440233.1"
                     /translation="MSYVRKLKSMIILSGALAFAGCSSTTAEPEPSAAAPASAEAKTK
                     VDSAKTTESTTKAPASSSLEALQRGESTATPASSPLKDVYFDFDRYDLREDAREILKA
                     NSAWLKANPAAQVQIEGHCDERGTTEYNVALGSRRAESAKDYLVTLGTSADHLSTISY
                     GEEVPVCREQTEECWQKNRRVRFVVQPGRPTT"
BASE COUNT         2170 a         2492 c         2628 g         2025 t
ORIGIN      
        1 caacatgatt tcactgccat gggagtacgc cgatcagacg ctttatgcgg cctatgaaaa
       61 aaccaaagcg caaggccgga gcggctttga aaattttcac gagttgcgcg ccagcatcgc
      121 cgcgggcaag cccaagcagc aggaacatcc gatctatcgc cggctcgatc ctgatagcgt
      181 caccggcggc agctggcgcg aacggtcgcg atttttgctg gaagagccgg aattgcactt
      241 ttggtttctg gatgaggatt gggtgacgcc ttatctgtcg cagctcgacg aagcgaagag
      301 cagccgtttg gtgctcaatc aggtgcagaa ggaagaacgc ctggctggga ttgtgcgcga
      361 tgcggtgaaa gccttttgcg ccggcgaaat cggcgccgtg ctgcaacgcc gtatggaaga
      421 catggcgctc tattttctcg aaaccggccg cgatgatgcg gcgcggctct cactggcagt
      481 ggctatccag atcggcaccg gggaccctgg gccgctggat atctcattcc tcaccgggct
      541 ggtgcagaag agttttggat actatctatc gcaacaaaag aacaaagagg ccgaggagcc
      601 ttcattcatc gtcaaaccct agcgttcatt aacgcccgac cgccaatttt cggtctttct
      661 cgcgcgggac cttgaagccc gcgcttcgag ccgatgtgtg atagcgccgg atgtcgctcg
      721 gaatctccgt gacatcgtcg agcactttga agagatcgag atcgccttta gcgatggcct
      781 cctccttggc cagagtattt cgaaaccatt gaattagccc agaccaatag ctggatccca
      841 ccagatagat cggaaacgga cgaatctttt gcgtctgcac cagagtcaaa atctcggcca
      901 tttcatccag ggtcccaaag ccgcccggga aaactacaaa accggcacta tatttggcca
      961 gcatcacctt gcgcacgtaa aagtattcga attgcagtga aattgtctgg tacggattgg
     1021 gcttttgttc atgctccaaa aggatattca acccgacgct tgtaccgtgg gcttcgcagg
     1081 cgccgcgatt cgccgcttcc atggttcctg gaccgccacc ggtgaccacc gcgtagcctg
     1141 cttcgaccag tgcgcgcccc aacttgcgcg cctgatcgta atagcggctg gtaattcgcg
     1201 tacgcgcgct gccgaaaacc gtaacggcgg gggatccgat ctgattcata cggtcaaacc
     1261 caagcgcaaa ttcgcccatg atccggaaca tcctccacga gtcctcggtt agtttgtcca
     1321 gctcgaactt catctcttta cctgccatct tttcacctcc aactgcaatc cgttgcgccg
     1381 ctcgccccgc gctgatgagt ttatccggcg tctgcgggac atcgtgcaat gatacaccac
     1441 ttttttttcc gggatgccgg tatctttagc ttgtacgggt ttcctcatgc cggtgttccg
     1501 ggatgcgccg ggatgaaccc gcttgttcga cctgctaaag tgccggtcta gtgctcatga
     1561 atgtacaatc gattgcaaaa ttcagccacg cgtcaagcag ggaaagatgg agacaagcag
     1621 aaggctattg agagccgtta aatggttgat cgaccggctt gcagcctatt tttcgccggc
     1681 attcgattcg accgtggagg tgcgacttag ggttttttcc gcgctcaaag gactggccgc
     1741 gttggctgcg ctcggactga taatgctgtg tctgtacgct ttagttttga taccgttcac
     1801 gccaagtatc gccgatctac aaaaagccaa gatcgaccaa ccgagcatgt tgatttcgtc
     1861 tgatggcaag cgcatcgcaa cgtataaacc gatgaaccgc gagtgggtcc gcttgaaccg
     1921 ggtttccccg catgtcatca acgcgctcat tgccacggaa gatcaccgct tttatcaaca
     1981 tcacggggtt gacgtgcgcc gcacgttatc gggtgttttc cgcaccgtgc tcggcgatac
     2041 cgagggcggt tccacgctga cccagcaact cgcgcgcaac ttatatcccg aggagatcgg
     2101 ccgtgagcgg acgatcacga gaaaaatcaa agaaaccatt accgcgatca aaatcgaata
     2161 cgcttataca aaaaaagaga ttttagagac ctatctgaac agtatgccgt ttctgtacaa
     2221 cgcgttcggc atcgaaatgg ctgcgcgcac ctacttcgac aaatcggcgc agaagctcga
     2281 cgtaatcgaa agcgcgactt tgatcgcgat gttgaaaggc accagctatt acaaccccgt
     2341 gctcaacccc gagcgcgcgc ttcgtcgccg caacgtcgta ttgtcgcaaa tggtcaagcg
     2401 cggcgtattg agccgggcag aatttgagcg gttaaaaacc aggccgatac gtctcgactt
     2461 tgagcgccag ccggaagcga tcggcatcgc tcctcacctt gccgcgcaca ttcgtaaatg
     2521 gctgatcgat tgggccgacc gcaatgacca caatatttat gccgacgggc tgagagtgca
     2581 caccaccatt gactcgcggt tgcagaccat tgccaagcat gcagtcagcc gccaggcgga
     2641 ggccctgcaa gccgtcgcag atgtggaatg gggatcgagc tcaccgcgac tgatctcgac
     2701 aagcgctaag gcttacgtcg atatgcgccg gcgcgtgcag cccttcgctt acttttggaa
     2761 aagcaaaagc ggcttgctcg acgtgtttat ccgcgaatcc gcggcgtacc gcgctgcgat
     2821 tcaatctggc gcggagcctg gagcggcgct ggcgctgctc cagagcaata gcgaattcat
     2881 gagcaagctg cgcgccgcaa aaacccgctt gcaagccggg tttgtggcga tcgatccggc
     2941 caccggggaa ataaaggcct gggttggcag ccgcgacttc aagaccgatc agtacgatca
     3001 tgtggcgcaa gcccggcggc aaccgggttc gacttttaag gcatttgttt atggtgcggc
     3061 gcttgagcaa ggaatgtcgc ccaacaaacg atttaccgat agtccagtgg agattcgcct
     3121 gccggacgga actatctggc gcccgtccga cctcggcgaa ccgagtggcc ggcgaatgac
     3181 ggcccgcgag ggccttattt attcgaaaaa taccatcacg gttcaggtca tgcaggaaat
     3241 cggtgcaaaa aaaactgcag aacttgcgcg caagatggga atcaatcgaa gcaagctcga
     3301 cgaggtcccg gcattagcgt tgggaaccag cccggtaacg ccgttggaaa tggtttctgc
     3361 ttacagcacc attgccgcca gcggcgttta tcgccagccg actttcatca cccgcatcac
     3421 ggacaacaaa ggaaaggtgc tcgccgaatt cgctactcag gacaagcgcg ccatgaccga
     3481 aaaaactgtc ggagatctga tcaacatgtt gcgcggcgca gtcgaccaag ggacggggca
     3541 agcggtgcgc acgcagttcg gtttgcgcgg cgatttcgcc ggtaaaaccg gcacgacaca
     3601 aaataatagc gatggttggt ttatattgat gcatccgcgg ctggtggccg ggtcctgggt
     3661 cggtttcaat gacccacgcg tcacgatgcg cagcaattat tggggcgagg gcggacacaa
     3721 cgcgctgttg ctggttggtg actttttcca gcaaacgctt gcgtcacggt tgatcgacag
     3781 ccgggccgat tttccatttg accgatatta cgagtcgatc tgggatccct atttcgacat
     3841 ggccaaagaa tggttgggtg gcatgctcag aggttggttc ggtggcgaga agcccaagcc
     3901 cgcgcctttg ccacggactt cgcctcgccg cgacttacga tttgaagcgc cgggctctga
     3961 acaatcgcaa aaccagaaag agccgatggt ggagcaattc gggggtacaa gagaccgagg
     4021 agagcagcgg cagcttgaaa gggaagcgga gaaggatcgg ctgttggagc ggtggaggca
     4081 gaagcaacaa caacgcgagc aggagacccg cgtcaccgat tgatacggag atcttcagga
     4141 acggtgatgc gagtcaataa tcgcacgcgc tgcgcgcgct gtgagctcgg tgttaatgat
     4201 atatcatgcg atcaggccgc gttgccacat tgactgttac attgtatgga tgctgaggag
     4261 ggatttaaaa tggctgtaga aattatcgat atgcctcggt tggctcatga acaaaaacgc
     4321 cgcaaggtgg tatttaacac gccgcgcttt catgcatggg tgcactatta cccaaatccc
     4381 ggcgagcggg atgacatgca ctgtcacaac gcggatcaga ctttttatgt catagaaggt
     4441 gaatgcacga tgcatttccc tgatggcggg aaggccataa tgaaacctgg aatggctgcg
     4501 atgatcacgg gaggatcttt ctaccagctg gaaaacagcg gtgatggcgc catgatcatg
     4561 atgggtaatc gctccgggcc ttccgaagcc atccagcaca ttaactatga gctcagaaaa
     4621 gacatcaaaa aactcagcaa agaggaattg gagcgcgcca atattcccta tctgtcaatg
     4681 caggtgagag gttgaaaaag cgcttgatgc ttctaatggc ctctgaacgg ccggcgatag
     4741 ctatttagaa tcgcgcgagg ttcagtcgga tctttcttgg aataagttac cgatcccaag
     4801 gcgcgctagg tagcaaatat ctgtaggatc tgccgcgcgg gacacctgtg ccagctcgac
     4861 cgtcggctcg atcctgcagc gccgtagccg atgtttgcca aacctctcga tgcattagta
     4921 ccgttgcttg atcagcgcga aaagaacccc gttgtgcttt tcgttgaagc cctggatcca
     4981 ttttctacta tcggagcggt ctgacgagga tagatgggag atgttatgag acgagcgctc
     5041 ttaggtgctt tcattgggct aatgttgttg ggcgcggtga ccctccaata cgcgtatgcg
     5101 gcccaaccgc taactaaaat cgtcatgacg acgggctcat ttagcgagcg agaggccgcc
     5161 atgtacgtgg cccaggatca agggatcttc cggcgctatg gtttggactt gtcgtttgtc
     5221 catgtacgta atggtcctgt cggcatggcg gcgctcgccg gcggtgacac ccagctccac
     5281 gaaggctccg cgactggcgc ggtgctcggc tctgccgctg aaggaaccga tctcgtgttc
     5341 gtggctggtt tgatcaacaa gctcatcggc aacatcatgg cgtcgcccaa aataaaagcg
     5401 ccgtctgatt tgaagggcaa agtcatcggt gtcaccagcg ccagcggcgg cagctggatg
     5461 tttacgacgc tggctctgga gtactggggt ctcgatgcca aacgtgatgg cattaacttt
     5521 cgtatcctgg gtgacgaatc ggtgcgcagc caagctctca tcaacgacag catcgccgcc
     5581 acccacgtag gctatacctt tgcggcgccg cttaagagcc gagggtttac caatctcgcc
     5641 gacctagcgc agctgccaat tccctttcaa agcaccggcg tactgaccaa gcgcagcttc
     5701 atcaaggcat cgccggaggt ggtagagagc gttctccgca gcgtggtcga tgccatggcc
     5761 ttcatcgctc aaccggaaaa taaacaggcg gtccttaaga gtatcgccaa gggactgcgc
     5821 ttggccacag cagagcaagc tattgaaggc tacgaaagtc tgccgctact gtatgaccgg
     5881 cgtatctatc ccacggttga tggcatacgc aacgtcatcc ggctcctggg tcaaaccaac
     5941 gagaaaattc gccgtttaaa ggccgaagat ttagtcgatg atcgctttgt caaaaagttg
     6001 gaaaaagaag gccgcttctg agcgcataat cgctagacat ctcatcattg gttgattcgt
     6061 gcgtcgattc gcgtccgaat tggcatccga tacaggtagg taaaacccat atcgcaggct
     6121 cggtgtgccg tatgcttatt tttacttggg caggcgacca tctatcttag gcggcttcgg
     6181 ggaaaactgg aaagcccgga tttagttgct gtttggccat agccgcataa attccgcctt
     6241 gcccgagcag ttcttgatgg ctgccgtgtt cgacgatcat tccttgatcc aagacgtaaa
     6301 tgcaatcgca gttcatcacg gcaccgatat gatggctcac cagaacgacc gtgtgcttgg
     6361 tcttcaaaga attcaaagtg tcgatgacga tttgttcgtg ttcggaatcg agagcgctgg
     6421 tcggttcgtc aaggacgagg ataggagctt tggtgaggag cgcccgtgcc agagcaattc
     6481 tttggcgctg cccgcccgaa aggttcatcc cggagtcgag cagtgctgtt tggtattggt
     6541 ccggcagggc ttctatgaaa ttggtcgcgc cggccagtcg cgccgcctcg cgcatctgtt
     6601 cgtatgtcgc cgccgggcaa ccgtaagcaa tgttctcggc gatggtcgtc ggtaggatca
     6661 cgctgtcctg caaaaccaag gctatatggc gacggatatc tttgagcttg atttgcctga
     6721 gatcgtaatg atcaagctta acccttccaa cggtcggatc gtaaaagcgt aataacaggt
     6781 tgagcagagt ggtcttgccg gcgccgctcg agccgacaaa tgcgacactt tgcccgggtt
     6841 cgatgttcac gctgatgcgc tggagaattt tctgatcggc ggagtattcg aatccgacgg
     6901 cctcaaggat caggctgcgc ggctggaggg aaagggcagt tgcttcagcc gagtcggtaa
     6961 cgaccggatc acgatcaagc acttcgaaaa ctcgccgagc tccggccagg ccgctctcga
     7021 gattgaatct gaaaccggac atctgacaaa gcgggtcgta gatcatcccg agataggtca
     7081 taaagatcat cagctcgccc ggcgtcaatt ggtgccggtg cagaagcatc ccgccgtaag
     7141 tcaaaatcag cgacgctccg acagcaagaa tgacgcccac gctcaaacca taagccactt
     7201 cttgccggtg gataccgacc caggcgcgca cgcaacgccg cgcgctgctg ccaaagcgat
     7261 gatattcatc ttcctcgcgc ccgaacgctt gggtaaggac aatggccgac atggaacgtt
     7321 gtatcgaagt tgtaaacgcg ctgtcggatt ctcgagattt acgcgtcttc tcacttagcc
     7381 ggcgcccgaa gatcacatta acccacatga gcggcggcgc tatcgacaaa gcgatcaagg
     7441 tcaaggccgc gtttcttccg atcaacagcc cggcgatcac acagagagtg atcccggcga
     7501 ttaacaggtt aatcatcacc gtaagcaccg cctgaaatcc caaggtgtcg gtagtcagcc
     7561 ggaatagcga gtcgctcatc ggccgtgaac gatgatagtc gagatgcatc ccttgaagct
     7621 tgcgatacaa gtcaccgcgt actcgcaaca aaccgttgta gtggatgcgc ggagcgagca
     7681 ggcggcgtgc catggcaatc aactcatgag caaaccgcaa caccaatgcc ataatcccca
     7741 ggccgacgat gcggctaatc ggatcatccg gcagcggcgc caaaaagagg cggtgcatcc
     7801 agctgtccat ggtcggcgtt ccgagagcgg aatctaccag caccgccaac ggccaggctt
     7861 gcagcagtcc gagagcggta gagcaggcgg tgagaaatag gagcaaaagg atcagatgca
     7921 gatcatcgcg aaaatagcgg atggcacgga gaacgtcctg gagtgctttt gatttgaagc
     7981 cctcgagggg ttcatagatc atcctggttg aaaattccgc ggaagtttca gattaatgct
     8041 ggaagccgaa tacagttgga agagaaactg ctattatcga gaggcgcttg caaaacttcg
     8101 atgaatctcc tggttagatc tgtgctgatg ggatgccact gattgccgtt tgaaaccgta
     8161 ttataagagg agagctgggc gctgagaaga aaaaatatcg gcgacgagtt tcactcggct
     8221 tagaaccgga tctgcccccc ggcaagcgtc agaggctatg aggtcggcag cgattgcgcg
     8281 cctgcgcgca tttagacaag ggcaggggaa aagaaaagat gactaaaaac agcgcttagc
     8341 ggacttctag tttctgtttt taccgtaagg gactggagca cgccactgct ggagcaggga
     8401 tatttttctg ctccagcggc gctgcgagat tacgattcat cctacgtggt agggcgccca
     8461 ggctgaacga cgaatcggac tcggcggttt ttctgccagc attcctcggt ttgttcccga
     8521 cagacaggaa cttcttcgcc gtagctaatg gtggagaggt ggtcggcgga agtcccgagc
     8581 gtaaccaaat aatccttcgc actctcggcc cgccgcgagc ccagagctac attgtactct
     8641 gtggttccac gctcgtcaca gtgcccctcg atctgaactt gcgccgcagg attggccttc
     8701 aaccaggccg agttcgcctt tagaatttca cgggcgtctt cgcggagatc gtaccggtcg
     8761 aaatcgaagt aaacatcctt caaaggactg gatgcaggag tcgcggttga ctcaccccgc
     8821 tgaagtgctt cgaggctcga tgatgcaggt gctttggtcg ttgattcagt ggttttagca
     8881 gaatcgactt tcgtcttggc ttcggccgag gccggagcgg cagcggaagg ttcaggctcg
     8941 gccgtggtgg acgagcatcc ggcaaatgcc aaggcgcccg agagaatgat catcgatttc
     9001 aattttcgta cgtagctcat acctttttac ctcctgcttt gacgttatca atttggccat
     9061 ctttttaggt gctgcatcga agttaccaat cctgtcgacg cgaactctgg attcgcgtaa
     9121 tcgtcggcaa ctattggggc ctccaagaca tcttttcggt ctgtaagcat cagaaaaagg
     9181 ctagtcaaga tcaatgtaca acatcgcacg tcaaatttca cgctacgctg cgacacgatt
     9241 ccgtaatcac gatagcgttc gcaagccagc ggtcggaacc gaaccctatg atgggcacct
     9301 ggcggacagg cgtat
//
LOCUS       DASUVS010000460         7151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_425911, whole genome shotgun sequence.
ACCESSION   DASUVS010000460 DASUVS010000000
VERSION     DASUVS010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7151
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425911"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VF182_24165"
     CDS             complement(<1..956)
                     /locus_tag="VF182_24165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6440234.1"
                     /translation="MKFCLQLNPQVSIDKPGAQLMPTLIEQVRIADAVGFDAFSMGDH
                     YNIPGLQRLNQVPALARLCAEAKRCAVGTAVMLLGLRHPVTVASELASLDVLNDGKSF
                     FAFGLGYRDDELNAFNLIKAQRFHRFVEGIEIIKRLWTENNVNYDGTAFKLKNVSVDP
                     KPLQKPRPPIWIAANSDAAVARAATIGDGWMIGPHSAIDELERQVDLCRQTWRAAGKP
                     GSPDIPIIRETFVAKTRKEAAEKARPCLEQLYRTIYVKWKQNEAMSDSSELSWAFDRL
                     SKGRFILGSPEECAEQIKEYGNRLGTDYILPRFDWTPGLPQE"
     gene            complement(972..1964)
                     /locus_tag="VF182_24170"
     CDS             complement(972..1964)
                     /locus_tag="VF182_24170"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440235.1"
                     /translation="MKRLIWLFSCCIVLGLAYPYPAPAEALRVGVSGLSAEFTPVWAA
                     SERGIFKKYGFESEVIAMQGGTQLAQAIIGGTIPIGVMGGAYLTAAVRGADLVMIATH
                     MDKFPYSLIVKPAIKKTEELKGTKLAISRFGSASDAGLRVSLQKLGLSPDKDVTILQV
                     GGQTSRFAALKAGTVDGTVVIPPLSGAAQRLGYNAVINMTKLGIPYPQEGVVVTRARL
                     ASRRDTVMRFLKAYLEGVRELKIDKEFAIFVMAKYLRMDPKKDREALEDSFQEVIVEQ
                     MLKVPQINLEATKVALDLLGKERPANASTDPRDYVDGSLMQELIKSGFTDNLYR"
     gene            complement(1990..3258)
                     /locus_tag="VF182_24175"
     CDS             complement(1990..3258)
                     /locus_tag="VF182_24175"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6440236.1"
                     /translation="MEQNPIFDKVSAIIERARAELIDLCLQLGNISSPHGQERDVGEA
                     VLEWLKQYQIHGQLQFITERSVNAVATMSGSGRGPSLIFNAHMDTGPELPADASEDAR
                     KLETAWVEGEMLFGKGMINDKAQLCAFMIAMSALRRAGVQLQGDLTLTAVASETGNPS
                     IDEFQGIDFPGEGFGTKWLVDRGAVADYALVGETSGFGIVQAECGAAWFKIQVKGREV
                     YTPRYERGASIQENPNAFAKAAHVIQAFEDWAVQYEKRETIEFAGGTIIPKAQIVEAR
                     GGAGVGRPNATCDIFTDVRIAPGKNPLTVQRELAAAIAKVNLDCEISLFQFSRGHIAK
                     NAEPLIDAVRSAHRYLFDAEPPQPPSAETSMWRDLNVFNEVGIPSICYGPPRQRERLS
                     GAQNRAMKIDDLVQATKVYALSAMILCGIE"
     gene            complement(3281..5545)
                     /locus_tag="VF182_24180"
     CDS             complement(3281..5545)
                     /locus_tag="VF182_24180"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6440237.1"
                     /translation="MVIGNKASRVDGFDKVTGEARYTGDLNLPGLLEAKVLRSPFPHA
                     RIQSIDTQKAAARSGVVAILTRDDVNDINPFYGNCLRDRPLVALDKVRFVGEPVAVVA
                     AEDPLIAEEALSLIDVRYQELPCLATVEDALAPGAPLVHESTSGTGEFHDIATVGAAI
                     KPNVCHHEFFERGDVIRGFAAADEILEETFEFPMIYQYAMEPHTSVARVTDGGITLWS
                     SSAHPFLVRAELAHMFGLVHAKVEVVVPYVGGAYGSKSYFKIEPLIVAMARKTAGRPV
                     RLAQSVPEAMLTTRRHSATVRIKTGFKRDGTLVARDAEVVMDTGAYADNGPRVAKRAI
                     SRMIGPYKLDDCKVEVAAVYTNTVPAGSMRSIGGPQTIWALESHMDSIAHRLGLDPLE
                     FRMRNLLRRGEELKTGATTIDADLRQGIKVAADYVSWGQRSKRIGKGRGVAVGVSDSE
                     AMPVSVALVRLLADGSILLLAGTTEVGQGARTILSQIVARELDVPIESVIMRGTDTLA
                     TPFDRSTGASRSTTVMGSAVKAAALDLRQQLIAAAAEALDSNTASIILRNGTANCGDR
                     SLTYGEVVSRYFGMPGGELIGRGTMRPGQGLGPKLPLFWETGMGAAEISLDIETGAIQ
                     IDRYITVADVGKAINLVQAEGQDEGAAIQGLGHTLFESLQYENGQPLNANLVDYRVPR
                     FTDMPALFDSALIENEDGPGPYGAKGMGESGIVSIAPAIGNAIFQATGARIRTLPLTP
                     ERVWRALNELKRGM"
     gene            complement(5653..6684)
                     /locus_tag="VF182_24185"
     CDS             complement(5653..6684)
                     /locus_tag="VF182_24185"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX6440238.1"
                     /translation="MNGKELLQKHVVVEGHRDVYEQLYRTSVGESMPIRDAIAPRLIR
                     DGINLSVYAISGDSYSHSQNTGRYLETALENIDQFLQEAPKSEGMISMVRTRGDLPER
                     VEPGTIKFLLHFEGCMPLRGSIQNLRNFYRLGLRSLQPVWNFRNELGDGVWENRTGGG
                     LTNFGVEVIKEANRLGMIVDLSHMNREGFFQSLEAATAPLIVSHANACGMLDSVRNLA
                     DDQIKAIADQGGVVGILALPERVGKGEVGIEDMLRHMDYMIKLVGVEHLALGMDFVKY
                     DGPRTLKDRHHPLHKDPLIKGFEEIEDLPNLIDGLQKHGYLENEIALILGGNYLRVLK
                     TILPEQSII"
     gene            6735..>7151
                     /locus_tag="VF182_24190"
     CDS             6735..>7151
                     /locus_tag="VF182_24190"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440239.1"
                     /translation="MHYLLASCPLILVIRKPQLLYSAVTLALLLLFVSCKTSPDSDSS
                     TSSSHFRVNLGTEPPSLDWSLATDGVSFNVIANLMVGLTEFTADLKPAPVIAKSWNLA
                     DGGKRIIFHLRDDVLWSDGQKVRAQDFEYSWKRLLNP"
BASE COUNT         1478 a         2124 c         1946 g         1603 t
ORIGIN      
        1 tcctcctgcg gcaatcccgg cgtccagtcg aagcgcggca agatgtagtc ggttcccaac
       61 cggtttccgt attctttgat ctgctcggcg cactcctcgg gcgagccgag gatgaaacgc
      121 cccttactta gacgatcgaa ggcccagctc aattcgctcg aatcgctcat cgcttcgttc
      181 tgcttccatt tgacgtatat cgtccggtag agctgttcca aacagggacg cgccttttcc
      241 gccgcctctt ttcttgtctt agcgacaaac gtctcacgaa tgatcggtat gtcgggcgaa
      301 cctggcttcc cggcagcgcg ccaagtttgg cggcagagat ccacttggcg ttccaattcg
      361 tcgatcgctg aatggggccc gatcatccag ccatcgccta tggtcgcggc gcgggccact
      421 gcggcatcgg aattggcggc gatccaaatc ggcggccgcg gcttctgcag cggtttggga
      481 tcgacgctga cgtttttaag cttgaatgct gtgccgtcat aattcacatt attctcggtc
      541 cagagccgtt tgataatctc aattccttcg acgaaccgat gaaagcgctg cgccttgatg
      601 agattaaaag cgttcaattc atcatcacgg tagcccaggc cgaaggcgaa gaaagatttg
      661 ccatcattga gcacgtcgag actcgccagc tcgctggcga cggtgaccgg atgacgcagg
      721 cccaatagca tcaccgcggt gccgacggcg caacgcttcg cctcggcgca gagccgcgcc
      781 aaggcaggaa cttgattgag ccgctgcaaa ccgggaatgt tgtaatgatc gcccatggag
      841 aaagcgtcga agccaaccgc atcggcgatg cggacctgct caatcaacgt cggcatcagt
      901 tgcgcgcccg gcttgtcgat cgatacttga gggtttagtt gcaggcagaa tttcatcagt
      961 tctccagaac tttatctata gagattgtcg gtgaaaccgc tcttgatcag ctcctgcatc
     1021 aggcttccat cgacataatc tctgggatcg gtcgaggcat tcgccggccg ttctttgccg
     1081 agcaagtcca gcgccacttt ggtggcttcc agattgattt gcggaacttt cagcatctgt
     1141 tccacgatga cctcttggaa actgtcttcc agcgcctcac gatctttttt cggatccata
     1201 cggagatatt tggccataac aaaaatggcg aattctttat cgatcttgag ctctctgacg
     1261 ccttccagat aagcttttag aaatctcatg accgtatcgc ggcggctcgc tagccgtgcc
     1321 cgggtcacca ccacaccctc ttgcggatag ggaataccca gcttggtcat attgatgact
     1381 gcgttatatc ccagacgctg cgccgccccg ctgagcggcg gaatcaccac ggtgccatct
     1441 acggtgccgg ctttgagcgc ggcaaatcgc gatgtctgcc cgcccacctg caaaatggtc
     1501 acatccttgt ccggactaag gccgagtttc tgtagcgaaa cgcgcagccc ggcatcgctg
     1561 gcagagccga accggctgat ggcgagtttg gttcctttca actcttcggt ttttttgatc
     1621 gccggcttga cgatgagcga atacggaaac ttgtccatgt gggtggcgat catcaccaga
     1681 tccgcgccac gcacggcagc ggtcaagtag gctccaccca tgacaccgat gggaatagtg
     1741 ccgccgataa ttgcttgggc taattgagtg ccgccctgca tcgcgatcac ttcgctttcg
     1801 aaaccatatt ttttgaaaat cccgcgctcg cttgccgccc aaacgggagt gaattcggcg
     1861 ctcaaaccgg agactccgac cctgagagct tcagccggtg ccggataggg atatgctaaa
     1921 ccgagcacga tgcagcaaga gaacaaccag atgagtcgct tcacaggcac ctctggcaat
     1981 acaggttcgt cattcgatgc cgcacaaaat catcgcgctc agcgcgtaga ccttggtcgc
     2041 ttgaacgagg tcgtcgatct tcatcgcccg gttctgggcg ccgctcagcc gctccctttg
     2101 ccggggcggc ccatagcaga tggacggaat accgacttcg ttgaaaacgt tcaggtcgcg
     2161 ccacatgctg gtttccgctg atggtggttg cggcggttcg gcatcgaaga gatagcggtg
     2221 cgccgatcta acggcatcga tcaacggctc ggcattcttg gcgatgtgcc cgcgggaaaa
     2281 ctgaaacaag gaaatctcac aatcgagatt taccttcgcg atagcagcgg caagctccct
     2341 ttgaaccgtg agtggattct tgccgggcgc aattctcacg tcggtgaaga tatcacaggt
     2401 ggcatttggc cggccgactc cggcgccgcc gcgcgcctcg acgatttgcg ctttgggtat
     2461 gatggtcccg ccggcaaact cgatagtttc tcgcttctca tactgcaccg cccagtcctc
     2521 gaaggcctga atcacatgag cagccttggc aaatgcgttg ggattctcct gaatagatgc
     2581 gcctcgctcg taacgcgggg tatagacctc tctgcctttg acttggatct tgaaccacgc
     2641 cgcgccacat tcggcctgga cgatgccgaa acctgaggtc tcgccgacca gggcataatc
     2701 ggcgacggcg ccgcgatcga cgagccattt ggtgccgaat ccctcgccgg gaaaatcgat
     2761 gccctgaaac tcatcgatcg atggattgcc ggtttcggac gccaccgccg tcagggtcag
     2821 atctccctgg agctgaactc cggcgcgcct gagcgcgctc atggcgatca tgaaggcgca
     2881 tagctgcgcc ttatcattga tcattccctt gccaaaaagc atctcgcctt caacccatgc
     2941 ggtctcgagc tttctggcat cctcgctggc gtcagccggc agctccggac cggtatccat
     3001 gtgcgcgttg aaaataagac tgggccctcg accgctgccc gacatagtcg caaccgcgtt
     3061 aacacttcgt tcggtgataa actgcagttg gccgtggatt tgatactgct tcaaccattc
     3121 gagcactgcc tctccgacat cgcgctcctg gccatgggga ctcgagatat tgcccaactg
     3181 caaacagaga tcgatcagct cggcgcgcgc tcgttcgatg attgcgctga ctttatcgaa
     3241 tatcggattc tgttccattt ccaatgcgaa ttcctttgct tcacataccg cgttttaatt
     3301 cattgagcgc gcgccagact ctttcagggg tcagcggcag agttcgaatc cgcgctccgg
     3361 tcgcttggaa gatcgcattg ccgattgccg gcgcgatcga gacgatgccc gattccccca
     3421 tccctttggc gccatagggg ccggggccgt cttcattttc gatcaaagcg gagtcgaaga
     3481 gcgccggcat atcggtgaac cgcggcacgc ggtaatcaac cagattggcg ttcaacggct
     3541 ggccgttctc gtactgcagt gactcaaaca aagtatgtcc cagtccctga atcgcagcgc
     3601 cttcatcctg accttcggct tgcacgagat tgatggcctt gccgacatcg gcaaccgtga
     3661 tgtagcgatc gatttggatc gcgccagtct cgatatccag tgaaatctcc gcggcgccca
     3721 tgccggtttc ccagaacagc ggcaacttcg gaccgagacc ctgaccgggc cgcatggtcc
     3781 cacggccgat gagttcgccg ccgggcatac cgaaatatct gctgaccact tcgccgtaag
     3841 tcagactacg atccccgcaa ttggccgtgc cgttgcgcaa aataattgaa gcagtattgc
     3901 tgtcgagcgc ttcggcggca gccgcaatca actgttgacg caaatcgaga gcagcagctt
     3961 tgacagcgct ccccatgact gtggttgagc ggctggcgcc ggtggaacga tcgaacgggg
     4021 ttgccagggt gtcggtcccg cgcatgatca cggattcgat cggaacatcc agctctcgag
     4081 cgacgatttg gctgaggatc gtgcgcgcgc cttgacccac ctccgtggtc ccggcaagca
     4141 agagaatgct gccgtccgca agaagccgca ccagcgcgac agaaaccggc atcgcttcgg
     4201 agtcggaaac cccaacggca acaccccggc ctttgccgat cctcttggag cgctggcccc
     4261 aagagacata atcagcggcg actttgattc cttgccgcag atcggcatca atcgtcgtgg
     4321 cgccggtttt taactcttca ccgcgcctga gcaaattgcg catgcgaaat tccaacgggt
     4381 ccaagccgag ccgatgagcg atgctgtcca tgtgcgattc caaggcccag atcgtctgtg
     4441 gcccgccgat ggagcgcatg gaaccagccg gaaccgtgtt ggtataaacc gctgcgactt
     4501 cgaccttgca atcgtccagt ttgtagggac caatcatgcg gctgatggcg cgcttggcga
     4561 cgcgcggccc gttgtcggca taggcgccgg tatccatcac cacctcggcg tcgcgcgcaa
     4621 ccagcgtccc gtcgcgtttg aaaccggtct tgatgcgcac tgtggcggag tggcgccgcg
     4681 tcgtcagcat cgcctccggc acgctttgag caagtcgcac aggccgaccg gcggtcttgc
     4741 gcgccatggc gacgatcaag ggttcgatct tgaaatagga tttgctgcca taagcgccgc
     4801 caacatacgg gacaaccact tcgaccttcg catgcaccaa gccgaacatg tgcgccagtt
     4861 cggcgcgaac caaaaacgga tgagccgaag agctccagag agtaatgccg ccgtcggtaa
     4921 cccgcgccac tgaggtgtgc ggctccatcg cgtactgata aatcatcggg aactcgaaag
     4981 tttcctcgag aatttcatcg gctgcggcaa aaccgcggat cacatcccct ctttcaaaga
     5041 attcgtgatg gcagacgttg ggctttatcg cggcgccgac ggtggcgatg tcatgaaatt
     5101 caccggtgcc ggacgtgctt tcgtgcacaa gcggtgcgcc tggcgcaagc gcatcttcaa
     5161 ccgttgcaag gcacggcagc tcttggtagc gcacgtcgat cagggatagc gcttcctcgg
     5221 cgatcaatgg atcttcagca gcgaccaccg ccactggctc gccgacaaac cgcaccttgt
     5281 ccaacgcgac cagcgggcga tcgcgcagac aattgccgta aaagggattg atgtcgttga
     5341 cgtcgtcgcg ggtcagaatc gccaccactc cggaacgagc ggctgctttc tgtgtgtcga
     5401 tcgattgaat gcgtgcgtga ggaaacgggc tgcgcaggac ttttgcctcc aacagtccgg
     5461 gcagattcag atcgccggtg tatctcgcct cgccggtaac tttgtcaaaa ccgtcgacgc
     5521 gagaagcctt attaccaatc accatcttga attaaactga ttgcgattca aaccagactg
     5581 ggtaacggtc gttgcgctcc tccggtcacg ccgttgatca tcacatggtc tccatctcga
     5641 ctgtgaaacg gattagatga tggactgctc aggaagaatc gtcttcaaga cccgcagata
     5701 gttgccgcca agaatcagag caatctcgtt ttcgagataa ccgtgcttct gcaggccgtc
     5761 aattagattg ggtaggtcct cgatctcttc gaatcctttg atcagcgggt ccttgtgcaa
     5821 cggatgatga cgatccttga gcgtgcgcgg cccgtcgtac ttgacgaaat ccatgcccag
     5881 agccagatgc tcgaccccaa cgagtttgat catgtagtcc atgtgcctca gcatgtcttc
     5941 gatgccgact tcgcctttgc cgacccgttc gggcaacgcc agaatgccta ccacgccgcc
     6001 ctgatcggca atcgctttga tctgatcgtc ggcaagattt ctcacgctgt cgagcatgcc
     6061 gcaggcattg gcatggctga cgatcagtgg cgcagtggcg gcctcgaggc tctggaaaaa
     6121 accctcccga ttcatgtgcg acaagtcgac gatcatgccc aggcgattgg cctccttaat
     6181 cacttcgacg ccgaagttcg tgagccctcc gccggttcga ttctcccaga cgccatcacc
     6241 gagctcgttg cgaaaattcc aaaccggctg cagcgagcgc agtcccaggc gataaaagtt
     6301 tctgagattc tggatactgc cgcgcagcgg catgcagcct tcgaagtgga gcaaaaattt
     6361 tattgtgccg ggctcgaccc tctccggcag atcgccgcgg gttctgacca tcgagatcat
     6421 gccctcggat ttgggcgctt cctgcagaaa ctgatcgatg ttttccagcg cggtttcgag
     6481 gtaacggccg gtattttgcg aatgggaata agaatcgccg ctgatagcat agacggacag
     6541 attgatgccg tcgcgaatca atcgaggcgc aatcgcatcg cggatcggca tcgattcgcc
     6601 gaccgacgtt ctgtagagct gctcataaac atcccgatgt ccttcgacca cgacgtgttt
     6661 ttgcagaagc tctttgccgt tcatagattc tcccatcttt tcttgaagat ctgcgcgcct
     6721 tcggatagta atagttgcac tatttattag caagctgtcc cttgattctc gtcatcagaa
     6781 aaccgcaatt gctctactcc gctgtcactc tggcgctgct gctgcttttc gtctcttgca
     6841 agacctcacc ggactccgat tcgagtactt ccagcagtca ttttcgcgtt aatttgggca
     6901 ccgagccgcc gagcctggat tggtccttgg ccaccgatgg agtttctttt aacgtcatcg
     6961 ccaacttgat ggttggcctc accgaattta ccgcagatct aaaacccgcg ccggtgatcg
     7021 ccaagtcttg gaatctagcc gatggcggca agcggatcat tttccatctg cgtgatgatg
     7081 tcttgtggag tgacggtcag aaagtccgcg ctcaagactt cgagtactct tggaagaggc
     7141 ttctcaaccc t
//
LOCUS       DASUVS010000461        15384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_205548, whole genome shotgun sequence.
ACCESSION   DASUVS010000461 DASUVS010000000
VERSION     DASUVS010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 15384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15384
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205548"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..673)
                     /locus_tag="VF182_24195"
     CDS             complement(119..673)
                     /locus_tag="VF182_24195"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440240.1"
                     /translation="MKLLRFKSLIPAVTLLSLLLISGMTSAADKLSLLYSAQSVSYSM
                     PWIAQDAGLFRKHDLDVKLVYIPSSGVATAALLGGDIEIALAGAVGIVRAFAQGAHDL
                     VFIGGFKNELTHNIVARPEIRRSQDLKGKVIGVTRLGSNSHYFAVQALPRFGLDPARD
                     VIFRQIGGVLPLSRRSSTALSMLW"
     gene            complement(791..2473)
                     /locus_tag="VF182_24200"
     CDS             complement(791..2473)
                     /locus_tag="VF182_24200"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6440241.1"
                     /translation="MTDLGQSLDCDVLIIGGGLAACMAAIEASKRGADVILVDKGRLG
                     RSGSSPTSGGVPQAAFGHADPNDSKEAHFRDTIIGGDYIPHQKIVRAVVSEITDRIVE
                     LEEIGLHFKKTADGKKFYQEKRLGSSFARSVPPIGGSVGMMGTLRKEVFNREVNVHQW
                     TMITKLFKDQDRVAGALGLNVQTGATRLYKAKAIIMAAGSAVALQKYTSANFQTTGDA
                     YVAAFDIGAELANLEFLEFTLIPAPKGITVPMAGLSPFTSKGGRFLNAAGERFLEKYD
                     PERLEGTTRAILVQATYKEMLAGHGPISIDPSFIPDEKWDDEIQYEYAPKLSAAGIDC
                     RRDRFEWVPALHTFLGGIFINERCESGVSGLFACGESSTGMHGSNRLSGNAIASCMVL
                     GARSGKFAALFAGANDFATLDQTEASEEIKRLESFHGESKLDPHDLELEIKTLAWESA
                     GVVRNETGLKAGVERFEEIRRDKIPLLKGNDQRGWIKALNCANLAWVGEMVTRSALER
                     RESRGQHFRDDYPTKEDGLPRWIKVSKKGPTLKCKAETIPFDKGDLMPGAKG"
     gene            complement(2470..2691)
                     /locus_tag="VF182_24205"
     CDS             complement(2470..2691)
                     /locus_tag="VF182_24205"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6440242.1"
                     /translation="MPIKVDPNKCDGCGICVYDCGADVFRFNEKKDKVFPYGNKYCVN
                     CFLCELVCPEDAIEVVLTTAKTNGKTSVP"
     gene            complement(2704..5481)
                     /locus_tag="VF182_24210"
     CDS             complement(2704..5481)
                     /locus_tag="VF182_24210"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6440243.1"
                     /translation="MADRSSIRPLRFELNAQWREATVEPQTLLADFLRDQLGLKGTRK
                     SCEVQICGACTVLVDGRPVSSCMTPAFEIHNKSVTTIEGLAQGDKLHPIQQAFWQEGG
                     FECGYCTPGMILTAYALLRENPDPSEEEIKHCLSGNICRCTGYVTIIAAIKRAACLMR
                     SDSTAIHSLSKSDDLRIDGPVKIRGEAIYTADLVRPGMLYGKILRSPLPHAIIKNIDA
                     IAAERLPGVVTVLTRDDLRDINPYFGPLVKDQTILAFDRVRYEGDPVAAVAAVSEEIA
                     AEALGLIHVAYEELKPLLSIDEALADDAPKIHEVTSEHGEKFPGYPSVDEEARKYRNV
                     SFHFGWNKGDIARGFAESHRVFDNSFYFSKVAHYSLEPHMVLAEWRDEAVTLWSSTQH
                     PFLVQQEIAEMFGIAKEKVRIIVPYVGGAYGNKNHTKFEPLATALSRKTRRPVYLSLS
                     TEDTFRTVSKPAMRVRIKTGVSQDGVLIARESVVHVDGGAYSDAGPRVTQKAGYRVHG
                     PYRIQNIKSDAYTVYTNTVPAGAFRGMGTPQVVWAYESQMDMIADELGLDPVEFRLKN
                     LLERGDDFSPGDTPLDCDLKEGLRRVAREIGWGEPLEPDCGIGVSCALKDGGGNYKIS
                     EARIEIDGEGKVTLFEGTVEIGQGSNTALRKIAAQELGLAAEKIELAPLDTSHTPLDF
                     GTYASSGTTVMGLAVQRAAQALKTQLIDGARSLTGRSAATFELNNGFLRVGEIAVSFQ
                     EVVRHVKGTIGTMSGEGRYESIRDKSLLLGAKAPFWEVSWGAAKVKVDRVTGQIKILK
                     FVTIADAGKAINPQQCYSQEIGAMVQGIGQAFFEQTLYENGIMLNPGLINYRVPGVYD
                     LPDELMTILFENGNGPGPYGVKGIGESGLLTVPSAIGNALFSAVGVRLLELPLTPERV
                     WRALHQGYFNQ"
     gene            complement(5517..6560)
                     /locus_tag="VF182_24215"
     CDS             complement(5517..6560)
                     /locus_tag="VF182_24215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440244.1"
                     /translation="MRIRPAYFAIIFLWLAALSDQSISPASAQEAFFQGKTIRIIVGF
                     SAGGGFDVYSRTIARHMGKHIPGSPSVLVENMPGAGSMIAANHLYNQAKPDGLTIGNW
                     IGGLILQQALGAKGIAFDAMKFEWIGAPVRINNACLLARKSGITSLQQWMQSKTLVKI
                     GAEAAGSTTSDIPRLLMKYTQLPLQLIEGYKGVADVRIATEAGEVAGFCSSWEGTKTS
                     WRSVLESKEASVILQAVPQPHADHPKAPLAIDQIKSKEGRELFKLVVHDIGGTINRLY
                     ALSPGTPKDRVKILQKAFQDTMKDPEFLAEAKKVRLDIDPLTGEEIDKTVAGVKNIPA
                     ASLATLRDILLPK"
     gene            complement(6686..6814)
                     /locus_tag="VF182_24220"
     CDS             complement(6686..6814)
                     /locus_tag="VF182_24220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440245.1"
                     /translation="MKLATANGDFLQRQMGINAKATIQNIFTATKVHVMAQPGEVF"
     gene            6947..7822
                     /locus_tag="VF182_24225"
     CDS             6947..7822
                     /locus_tag="VF182_24225"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6440246.1"
                     /translation="MLEEIKRARDFAWPAGIRLPVVLTFEHQAGEGALSMPGNRPNYR
                     SGDIMQYGARQGIWNILEALEKYSVKATFLFSGSTAQNYPETARAVIQVEHEIAGRGF
                     NFESIHTSSAERERSIVRRTAAVLEDVTGTRIQGWRCPDYRVSPQTHDVLADEGFLWD
                     SSLLNDDLPYLLACGTRRLVEIPFTTSTADKAFVGDPPPQRGGPHGLANAWDSEFALL
                     YEESQRAPRFLILSLQTWASGRPVVLRILTKFLERLSSLQGIWFARCCEIARLLEPNK
                     QRERELEFDVERKTS"
     gene            7800..8684
                     /locus_tag="VF182_24230"
     CDS             7800..8684
                     /locus_tag="VF182_24230"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6440247.1"
                     /translation="MWKEKHPERKARWPEGVRLPIMVSVHHQSEEAPCPLADGQPDPF
                     DFSERQYGGRRGAWRLLEILAKHEIKATWFICGATARRYPQISRAAKTAGHCIAGHGY
                     HHEFMCNLPPEAELRIIKRTVAAFQDVVGEELKGWRTCFPSHNTIDILLEHFHFDWDC
                     SRRNDDKPYILQGYGRDMVEIPFIYDADSSLSVRITNPQPPNSSNIWDCNPPEFTLRA
                     MKAWFDALYESGRERPLMLPLTVHDFITGRPSRAKALDDFLSYAQQFPGAIFTTHDEL
                     ASWWRSNYERHEPDEGTK"
     gene            8813..9826
                     /locus_tag="VF182_24235"
     CDS             8813..9826
                     /locus_tag="VF182_24235"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440248.1"
                     /translation="MKSAARLRFYSLLVISLSVELFLVPSLVQNPGAQNSMLTVGQTG
                     INPGTSLYFIAQKEKFYLKHGLDVNIIATNTTAAVQAMLGGTMQMSTGSGAAAFVTAT
                     AEGAPGFVLVSSWVNVFPYTVMVQQDIKTPQDLRGKVGHVGAPFGAIPDVALRFALTK
                     LGLDPEKDVRLVQIARPDPANIIAQMERGDVQFGPLAPPFDRIAEKRGFRPLISLPEL
                     GIPWQNNGEWVQRSYLKSNRDEVLKFTRAIADAMKFYFEQKEKSVAYLAEFLAIDRAD
                     AEYAYQAYAKWVDRVPRPKAEGIRTTIEAIKKTTPKAGAADAGSFIDASLVDQLVKEG
                     YIK"
     gene            complement(9904..10518)
                     /locus_tag="VF182_24240"
     CDS             complement(9904..10518)
                     /locus_tag="VF182_24240"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX6440249.1"
                     /translation="MEVEQLLQISPENSALIIVDMQAEGCERHGSTLKPVSKNIRALL
                     DRFRHVNGNIIHVQSVRAKDHPEFTVFGKEYSLIENSPSVEFVDELKPLQNEIVVQKF
                     SHDCFYRTTMEEELRRLDLRSCRDRIVVTGIGSNNCVYHAVIGFHIRNYFVIVPEDCI
                     HASRPEGQQFALSQFRSPAYNFNVTVARSEDIKIAGGVKKRALG"
     gene            complement(10548..11495)
                     /locus_tag="VF182_24245"
     CDS             complement(10548..11495)
                     /locus_tag="VF182_24245"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440250.1"
                     /translation="MKRKLLIFLALLFISAKLTNAAELQVITVALVSPSWSTGLPTAV
                     ARGAGFFRNEGLEVRPVTLASSGPIMMALLMSGQAEMVIAGGVAILRGIARGAPVVVI
                     GGHLSRMSYALISAKGLKTVDDLKGKAIGITGIGGIGEFAVVESLKRNGLIKDRDYSL
                     LNIEGGTAARMAALKAGKVHAVPVTPGQRVQAENDGFTILLDVRDSLADLPSNIVAST
                     KDFARSNPDKTIRFLRALGKAMDLIRQDKDKAIALGRTNGLRGDAAMERKALDYYVQD
                     LDIGLNKNNVEALLRLLDISDPAEKFFDDTYLKRAIPRP"
     gene            11704..12483
                     /locus_tag="VF182_24250"
     CDS             11704..12483
                     /locus_tag="VF182_24250"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6440251.1"
                     /translation="MSQIRHMANCTNNNRRLARFYQLILGMEEVWNEEQNSPYAFYVT
                     DGYFNLNCLQIHQTIAEVKRDVGINHFGFKVDSLNEIKTKLSQLDPPISLGSRPNDGR
                     YTEVRILDPDGNGVDVAERGWGTRDDKNLPGVRYVGIKTTNPERLAEFYKFIFSMKEV
                     DRMDNPRTESRAIYLSDGTINLGLIMNPPVPREGLQVLGFQVKSIREVEERLKQARGL
                     TYKGEAPLAIEPSLLAGPYKTVSLKDPDGTHLDLSEEGWTV"
     gene            12535..13530
                     /locus_tag="VF182_24255"
     CDS             12535..13530
                     /locus_tag="VF182_24255"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440252.1"
                     /translation="MQWVVFNLICVIGFFGAVSTAAGQALKVNVSYTGVGPTQLPVWV
                     AKETGIFSKNGLDVQLIRAQAATSVMALLSGELNLIQGAAPAVVQSNLRGFGTVYVAA
                     GYVGFEYWLMSQRDIKSAEQLKGGLLGVSGLTGASATATQLVLRKLGLVPGKDTSVIV
                     IGGTPERLIALRSGKIQATLLNPPTTFVAQREGFNVLADLGTLPFQNNGVVTTRKFIN
                     EKPEAVRRYVRSQIEAVHLMKTERATGLKVLAKYMGGTSDQELLEKSYDASVTEHTFP
                     AKQYPSQAGLKTVIDSLVADNPKAKEAKPEDFIDSQFIKELDERGFIDNLKNKKS"
     gene            13784..14179
                     /locus_tag="VF182_24260"
     CDS             13784..14179
                     /locus_tag="VF182_24260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440253.1"
                     /translation="MENTKTDPIVIEQPGMRMTIAHRSGLGGNDEGLTFDVTTVGSEN
                     EGKRILRFDCFYKNPHYHVGTSGGESAAYKMKEEGIEDAVRWTLEQLKTRFPAMVKQA
                     GYEEIAATIDQQAVTDQLSRLEREIVAKY"
     gene            14330..>15384
                     /locus_tag="VF182_24265"
     CDS             14330..>15384
                     /locus_tag="VF182_24265"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6440254.1"
                     /translation="MLLLTNNDVEQVLTMADCVDVLRRFFEEEGKGHVLTRQRTESWL
                     PHLTTDTFYQCKTMEGGVPYIGKYVIRIDSNVTRKKQLGLSSRVEHVPFGNGSWMGML
                     LVFSTETGELLGWMPDGYLQRTRVGALYALAADYLARPDAEEVGLIGSGWQAGGQILG
                     LRCVRDINKVRVFSSNAARRSRFAAELQASLGIEVRPIGAARDAFANANIVALATNAN
                     EKVMESSWIEPGQHLSSVRVLELDPRSYEECDRVVVNRIEPWIQNYYLGQLCPQDIKD
                     AKVPPAPLRAIEAGEFFRQRIKRTGAREKTLFPNEASNYQLGGQFAAVAGFVLDRARE
                     KGLGRDLPAEWFAQTLVP"
BASE COUNT         3482 a         4135 c         4187 g         3580 t
ORIGIN      
        1 gagctatcac cgatcgacgc gtagtcacgc tcgcagcggc gtacgggata tgcaggtcgc
       61 gacccgttga tgacgtagtg aaagccttgc gcaacggcgc tttgaccgta agtaagcatc
      121 accatagcat cgatagtgcc gttgatgagc gccgcgagag cggcaacaca ccgcctattt
      181 gacggaagat tacatcgcga gctggatcca agccgaagcg tggcagcgct tgcaccgcga
      241 agtagtggct gttgctgcca aggcgggtga caccgatgac tttgccttta aggtcctgcg
      301 atcgcctgat ttccggtcgc gcgacaatgt tgtgcgtgag ctcgttcttg aagccgccga
      361 tgaacaccaa atcgtgggcg ccctgagcga aggcgcgcac aatgccgacc gctccagcta
      421 aagctatttc aatgtcacca ccgaggagag ccgcggtggc cacgccggag gaggggatgt
      481 agaccaattt aacgtccagg tcgtgcttgc gaaataatcc ggcgtcctgc gcgatccagg
      541 gcatcgagta cgataccgac tgagcggaat agagcaagct gagcttgtcg gccgctgagg
      601 tcatgcctga gatcagcaga agagataaga gtgtaacggc gggtatcagc gatttgaaac
      661 gcaaaagctt cacattattc ggccgttcac gagcaagaag aaaatgtctc taagtaaaga
      721 cgtagccgga tgaccggacg ccaggatcat caacttctag gataaatgat tcctctccgg
      781 ccagcgtcag ttatccttta gccccaggca tcaggtcgcc tttgtcaaac gggattgtct
      841 ccgctttaca cttgagagtc ggcccttttt tggagacttt aatccacctc gggagaccgt
      901 cttctttggt agggtaatcg tcgcgaaagt gctggcctct agattcccgt cgttctaatg
      961 cgctccttgt gaccatttct ccaacccatg ccaggttcgc gcagttcaac gctttgatcc
     1021 agccgcgttg atcgttgccc tttaacagcg ggatcttgtc gcggcgaatc tcttcaaacc
     1081 gctcaacgcc tgccttgagc cccgtttcat ttctcacgac gccagccgac tcccaggcga
     1141 gggttttaat ctccagctct agatcgtgcg gatccaattt actctcgccg tgaaaacttt
     1201 cgagccgctt tatctcctct gatgcctccg tttggtcaag cgttgcaaaa tcgttggcgc
     1261 cggcgaaaag tgccgcaaat tttccggacc gagcgccgag aaccatgcaa cttgcgatgg
     1321 cattgccgga tagcctattc gatccatgca tgccggtgga tgactctccg caagcaaaca
     1381 gcccggagac tccggattcg catcgttcgt tgatgaaaat accgccgaga aacgtatgaa
     1441 gcgccggcac ccattcaaat cggtcgcggc ggcagtcgat gccggcggcc gacaatttgg
     1501 gcgcatactc atattggatc tcatcatccc atttctcgtc gggaatgaac gaggggtcga
     1561 tcgagatcgg cccatgcccg gcgagcatct ctttgtaagt ggcctgtacg agaatcgctc
     1621 gagttgtgcc ctcaagtctt tccggatcgt acttttccaa gaaacgctct ccggccgcat
     1681 tgagaaatcg cccgcctttg ctggtgaaag gcgacagtcc ggccatcgga acggtgattc
     1741 ctttgggagc cgggattagc gtgaattcta aaaactccag gtttgcgagc tcggcaccga
     1801 tatcgaaggc cgccacgtaa gcatcgccgg tagtctggaa gtttgcgctg gtatattttt
     1861 gtagagctac ggcgctgccg gccgccatta ttatagcttt ggctttgtac aggcgggttg
     1921 cgccggtctg aacatttagc ccgagcgcgc cggcaactcg atcctggtct ttgaatagct
     1981 tcgtgatcat cgtccactga tgaacgttga cctcgcgatt aaaaacttct ttcctgagag
     2041 tacccatcat gccgacgctg ccgccaatcg gcggaacgct gcgcgcgaac gagcttccca
     2101 gtcttttctc ctggtaaaac ttctttccat cggcggtttt tttgaagtga agaccgattt
     2161 cttcgagctc tacaatccga tcggtgattt ccgaaaccac tgcgcgaacg attttctgat
     2221 ggggaatgta gtcgccgccg atgatggtat cgcggaaatg cgcctcctta ctgtcattcg
     2281 ggtccgcatg gccgaaggcg gcctgcggca ctcctcccga tgtcgggctg gagccgctcc
     2341 tcccgagtct gcccttgtcg accagaatca catcggcgcc gcgctttgac gcttcgatgg
     2401 ccgccatgca ggcggctagg ccgccgccga tgatcaacac gtcacagtct aatgattggc
     2461 cgagatcggt catggaacgg aagtcttacc gtttgttttt gccgtcgtta ggactacttc
     2521 gatcgcgtct tcagggcaca ccagttcgca aagaaaacag ttgacgcagt acttgttacc
     2581 gtacgggaaa actttgtcct ttttttcgtt aaagcgaaaa acgtcagcgc cgcaatcgta
     2641 aacgcagatg ccgcagccat cacatttgtt ggggtctact ttgataggca atagaaatct
     2701 ccgtcactgg ttgaagtatc cttggtggag cgcgcgccag accctctccg gcgtcagagg
     2761 aagctctaag aggcgaaccc caaccgctga gaagagggca ttaccgatag ccgacggcac
     2821 ggtaagcaaa ccgctctcgc cgatgccctt tactccgtaa ggacccggtc cgttgccgtt
     2881 ctcgaacaag attgtcatca gctcatccgg caggtcataa actcccggga cgcggtagtt
     2941 gattaaaccg gggttcagca tgatgccgtt ttcatacagg gtctgctcaa aaaatgcttg
     3001 accgattcct tgtaccatcg cgccgatttc ctgcgagtaa cattgctgtg gattaatcgc
     3061 cttaccggca tcggcgatcg tgacgaattt caaaatttta atctgacccg tgacgcgatc
     3121 caccttgact ttggcggcgc cccagctgac ttcccagaac ggcgccttag cgcccaggag
     3181 aagactcttg tctctaatgc tttcgtagcg cccttcgccg ctcatggttc caatcgtgcc
     3241 cttaacgtgc cggaccacct cctggaagct gacagcaatt tcgccgacac gcaagaaacc
     3301 attattcagt tcgaaggttg cagcgctccg accggtcaag cttctggcgc cgtcgatgag
     3361 ctgagtcttg agcgcctgcg cagctctttg aacggcgagg cccataacgg tcgtaccgct
     3421 gcttgcgtag gtgccgaagt cgagcggtgt gtgtgaggta tccagaggag caagttcgat
     3481 tttctctgca gcaagaccga gttcttgggc ggcaattttc cgcagcgctg tgttcgatcc
     3541 ctgaccgatc tccaccgtac cctcgaaaag cgtcacttta ccttccccgt cgatttcgat
     3601 tcgtgcctcg gaaatcttgt agttgccgcc gccgtctttc agagcgcaac tcacgccgat
     3661 accgcagtcc ggctccaatg gctcacccca tcctatctct cgagcgacac gccgcaggcc
     3721 ttccttcaag tcgcaatcca gcggtgtgtc gccgggcgag aagtcgtcgc cgcgctccag
     3781 gagattcttg agacgaaatt ccacgggatc caaacccagc tcgtcggcga tcatgtccat
     3841 ttgtgactcg taggcccaga ctacttgcgg cgtgcccatg ccgcggaatg cgcccgcggg
     3901 tactgtgttg gtgtacacgg tataggcgtc ggatttgatg ttttgaatgc gataggggcc
     3961 gtgcacgcgg tagcccgcct tctgtgtgac tcgcggtccg gcatcagaat aggccccgcc
     4021 atcgacatgg acaacggact ctcgcgcaat tagaacgcca tcctgactca cgccggtttt
     4081 tatccgtaca cgcatggccg gtttgctcac ggtgcgaaag gtatcttccg tggaaagcga
     4141 gagatacacc ggtcgccgag tcttgcgcga cagcgcagtg gccagcggct cgaacttggt
     4201 gtggtttttg ttgccgtagg ccccgccgac ataggggacg ataatgcgga ccttctcttt
     4261 ggcaatgccg aacatttcgg caatttcctg ttgcaccagg aagggatgtt gtgtcgagct
     4321 ccagagggtc acagcctcgt cgcgccactc agccaaaacc atgtgcggtt ccagtgaata
     4381 gtgagcgact ttagaaaaat aaaaagagtt gtcaaacaca cggtgtgatt cggcaaaccc
     4441 tcgcgcaatg tcgcccttgt tccagccgaa atgaaaactt acgttgcgat atttcctggc
     4501 ttcttcatcg acactcgggt agccggggaa tttttccccg tgttcgctgg tgacttcatg
     4561 aatttttggc gcatcgtcag ccaaggcttc atcgatcgaa agtagaggtt tgagctcttc
     4621 gtaggcgacg tgaatgaggc caagggcttc tgcggcgatc tcctcactga cggccgcaac
     4681 cgccgcaacc gggtcacctt cgtaacgtac tctgtcaaac gcgagaatag tctggtcttt
     4741 gacgagcggg ccgaagtaag ggttgatgtc ccttagatcg tcgcgggtga gaacggtcac
     4801 gacgcccggc agcctctcgg cggctatcgc atcgatgttt ttgattatcg cgtgcggcaa
     4861 cggactgcgc aggattttac cgtacagcat accggggcga acgaggtcgg ccgtataaat
     4921 ggcctcgccg cggattttaa caggaccgtc gatgcgcaga tcgtctgatt tggacagtga
     4981 atgaattgct gtgctatcag accgcatcaa acaagctgcc cgtttgatag cggcaatgat
     5041 cgtcacatag cccgtacaac ggcagatatt gccgctcaag caatgtttaa tttcttcttc
     5101 gctgggatct gggttctcac ggagcaacgc atatgccgtc agaatcatgc ccggggtgca
     5161 gtaaccgcat tcaaatccgc cttcctgcca gaaggcttgt tgtatcggat ggagtttatc
     5221 gccttgagcc aagccttcga tggtcgtgac cgacttattg tggatctcaa atgcaggggt
     5281 catgcaggaa ctgacgggcc taccatcgac cagcacagta caggcgccgc aaatctgcac
     5341 ttcgcaactc ttcctcgttc ctttaagacc gagctgatcg cggagaaagt cggcaagtaa
     5401 agtctgaggt tcgacggtag cctcacgcca ctgagcattc aactcgaagc gtaatggtct
     5461 tatcgaagat ctatcggcca tcagcaggtt cgtaggtttc gttggagagc ttgtgtttat
     5521 tttggcagta ggatgtctct tagcgtggct aggcttgcgg cggggatgtt tttaactcca
     5581 gctacggttt tgtcgatttc ctccccggtc aagggatcga tatcgagcct gactttcttt
     5641 gcctcggcca ggaactcggg atccttcatg gtatcctgaa atgccttctg taaaatcttc
     5701 acacggtcct tcggggtgcc gggcgacaat gcgtagagcc gattgatggt tccgccgatg
     5761 tcgtgaacca ccagtttaaa aagttcgcgc ccttcttttg atttaatttg atcgatggcc
     5821 aggggcgcct tgggatggtc cgcatgcggt tgcgggacgg cctgcagtat caccgaagct
     5881 tccttgcttt ccagcacgct gcgccacgat gttttggttc cttcccaact tgagcaaaag
     5941 ccggcaacct caccggcctc ggtcgcaatg cgaacatcgg caactccttt gtaaccttcg
     6001 atcaattgta atgggagctg ggtgtacttc atcagaagcc gcggaatatc cgaggtggtg
     6061 gaacccgccg cctcggcgcc gattttcacc aatgttttcg attgcatcca ttgctgcaag
     6121 ctggtgattc cgctcttgcg cgccagcagg caggcattat tgatccgtac cggagcgccg
     6181 atccattcga acttcatggc gtcgaaggct attcctttgg cgccgagagc ttgctgaagg
     6241 atgagcccgc cgatccaatt tccaatggta agaccatccg gcttggcttg attgtacaga
     6301 tgattcgccg cgatcatact ccccgcgcct ggcatgtttt cgaccaggac agacggtgat
     6361 ccgggaatat gtttgcccat atggcgggcg atcgtcctgg agtaaacatc gaaaccgccg
     6421 ccggcggaaa aaccgacgat gatgcggatt gtcttgccct gaaagaaagc ttcctgcgcg
     6481 ctggcaggtg agatcgattg gtcgcttaat gcagcgagcc atagaaagat aatcgcgaag
     6541 tatgccggcc ttatgcgcat tttgaaaacc ccctgtgaaa tatgtcggta accatactga
     6601 cacccggatg atccgtcaat gcatgttcag gtatgacatc attatcgtaa agattactcc
     6661 tggtcttaga cacgcaggga gtcgtttaaa atacttcgcc aggctgcgcc atgacgtgaa
     6721 ctttcgtcgc agtaaaaatg ttctgtattg tcgctttggc attgatgccc atctgccgtt
     6781 gtagaaaatc gccgttagcc gttgcgagtt tcacaccgtc cgtataaagg ttcgttctaa
     6841 caatcttgaa acgggcaggt ttaaagttgg tgatcaatgg tgtaacccgc agacaaaccg
     6901 gaatgttacc tcgatacgac ggtggcgaaa ttaaccggag ggatgaatgt tagaagaaat
     6961 caagcgggcc cgggactttg catggcctgc gggcattcgt ctaccggtgg ttctaacttt
     7021 cgaacatcaa gctggtgagg gagccttatc gatgcccggc aatcggccta attatcgatc
     7081 tggcgatatt atgcagtacg gcgcgcgtca aggaatttgg aacatactcg aagcgttgga
     7141 aaaatattct gtcaaggcaa ccttcttgtt ctcgggttcg acggcgcaga attatcccga
     7201 gaccgcccgc gcggtgatcc aggtggagca tgaaatcgcc ggccgtggct ttaattttga
     7261 gagcattcac acctctagcg ccgaacgcga aaggtctata gtgcgtcgga ccgctgccgt
     7321 tctggaagat gtgaccggaa ctcgaattca gggttggcgt tgtccggatt atcgcgtaag
     7381 cccgcaaacg cacgacgttc tggccgacga aggattttta tgggacagca gtcttctgaa
     7441 cgatgattta ccctacctgc tagcgtgcgg cacccgtcgc ctagtcgaaa ttccatttac
     7501 cacttcgacc gccgataagg ctttcgtcgg cgaccctccg ccgcagcgcg gaggaccgca
     7561 tggactggcc aacgcatggg atagcgagtt cgctctcctg tatgaagaga gtcaacgcgc
     7621 tccaagattt ttaatattga gcttgcaaac ttgggctagc ggccgtccgg tagtgcttag
     7681 aattttgaca aaatttctcg agcggctgtc atcgctgcaa ggaatttggt ttgctcgctg
     7741 ttgcgagatc gcacggctgc tcgaaccaaa taaacaacga gagcgcgaac tagaattcga
     7801 tgtggaaaga aaaacatcct gagcgtaagg cgcgatggcc ggagggtgtt cgcttaccga
     7861 taatggtaag cgtacatcat caatcagaag aggcgccgtg cccgctggcc gatggccagc
     7921 cggacccctt cgactttagt gaacggcaat acggcggacg ccggggtgct tggcggcttc
     7981 ttgaaattct cgccaagcac gagatcaaag ccacctggtt tatctgcggc gcgactgccc
     8041 ggcgctatcc gcagatttca cgagccgcta aaacagcggg gcactgcatt gccgggcatg
     8101 gctatcatca tgaattcatg tgcaacctgc cgcctgaggc ggagcttcgg attatcaaaa
     8161 ggacagttgc ggcatttcag gatgtcgtcg gcgaagagtt aaagggttgg cggacgtgct
     8221 ttccgagcca caataccatc gatatacttc tcgaacattt tcatttcgac tgggattgca
     8281 gccgtcgcaa tgatgataag ccctatattc tccaagggta tggacgggac atggtcgaaa
     8341 ttcctttcat ctacgatgcc gattcgtcgc tgtcggtgcg catcacaaat ccgcagccgc
     8401 ctaactcctc aaatatctgg gattgcaatc cgcccgagtt taccttgcgg gctatgaagg
     8461 cgtggtttga tgcgctctac gagagcggcc gcgagagacc gctcatgctg ccgttgacgg
     8521 tgcatgattt catcaccggc cgtccctcga gagcgaaagc cctagacgac tttctgtcgt
     8581 acgcgcagca atttcccgga gcgatcttca cgactcacga cgagctagcc tcgtggtggc
     8641 gatccaacta cgaacggcac gaaccggacg aaggcactaa atgagtttgc cgcgctcgac
     8701 ccagacatat tgattttgtc gcttacccaa acaatcgatt atcgccgggg aaagattccc
     8761 aggaagaatg ccgccgaggt ttacagatca tgtgctctaa ggaggtttct caatgaagtc
     8821 cgcagcacgt ttgcgcttct attcactcct agtcatctcg ctttcggtcg aacttttttt
     8881 agttccgtct ctagtacaga atccaggcgc acaaaactcg atgctcaccg ttggacagac
     8941 tggcataaac ccggggacga gcttgtattt tattgctcaa aaggaaaaat tttacctcaa
     9001 acatgggctg gatgtgaaca tcattgcgac caatacaacc gccgctgttc aggccatgtt
     9061 gggcggcact atgcaaatgt caactggttc cggagcagct gcgtttgtca ccgcgacggc
     9121 ggaaggagct ccgggattcg ttctggtttc ttcctgggtc aatgtttttc cttatacggt
     9181 gatggtgcaa caggatatca aaactccgca ggatctcaga ggcaaagtcg gtcatgtggg
     9241 cgcgcccttc ggcgccatcc ctgatgtggc cctgcggttc gcgctcacaa agcttggcct
     9301 cgatccagag aaggacgtga ggctagttca aatcgccagg cccgatcccg ctaacatcat
     9361 tgcccagatg gaaagaggcg acgtgcagtt tggtccgctt gcgcctccct ttgatcgcat
     9421 tgcggagaaa cgcggttttc gtccattgat ttcactgccg gagctcggca ttccatggca
     9481 gaacaatggt gaatgggtgc aaagatctta tcttaaatca aaccgggatg aagtcttgaa
     9541 atttactcgc gctatcgctg acgcgatgaa attttatttt gagcagaaag aaaaaagcgt
     9601 cgcctattta gcagaatttc tggcaatcga tcgcgcagac gcggagtatg cctatcaggc
     9661 ttatgcgaaa tgggtcgatc gagttcccag gcccaaggct gaggggatcc gcactacgat
     9721 cgaagcaatc aagaagacga cgcccaaagc aggcgcagcc gatgccggat catttatcga
     9781 tgcgagtttg gtcgatcaac tggtaaagga aggctacatc aaatagttca ggccgacttg
     9841 aagcctatcc ttgaaaaatc gcagctgcga taggtgttaa tgacgccgct cgactcgaat
     9901 tggctatccc aaggctctct ttttgacgcc gccggcaatc ttgatatctt ccgaccgcgc
     9961 caccgtcaca ttgaagttgt aagcaggcga ccggaactgg ctcaaagcaa attgttggcc
    10021 ttcgggacgg ctggcgtgaa tgcaatcctc cgggacgatc acgaagtaat ttctaatatg
    10081 aaatccgatg acggcgtgat agacgcagtt gttggagccg ataccggtca ccacgattcg
    10141 gtctctgcaa gagcgaagat ccagccttcg aagctcctct tccatggtgg tccgataaaa
    10201 gcagtcatgc gagaacttct gaaccacaat ttcgttctgt agtggcttta attcgtcaac
    10261 gaactcgacc gacgggctgt tttcgatcaa agaatactcc ttgccgaata ccgtaaattc
    10321 cggatgatct ttggcccgaa ccgactgaac gtgaataatg tttccgttta catgccgaaa
    10381 tctatctaga agagctcgga tatttttgct aaccggcttt agagtcgatc catgccgttc
    10441 gcagccttcc gcttgcatgt cgacgatgat taaagcgctg ttttcgggag aaatttgcaa
    10501 aagctgttcg acttccatgg agacacctcc agcagaatat ttgtagtcta tggtcgagga
    10561 atggcccgtt tcagataagt atcatcaaag aatttttccg caggatccga gatgtccagc
    10621 aatcttaaca aagcttcgac attattcttg ttgagcccga tatcgaggtc ctgaacgtag
    10681 taatcgagcg cttttcgttc catcgctgca tctccacgta acccattagt cctgccgagc
    10741 gctatcgctt tatccttatc ttgccggatc aaatccatag ctttgccgag agccctaaga
    10801 aagcgaatgg ttttgtccgg gttggacctc gcaaagtcct tcgtcgacgc gacaatgttc
    10861 gacggaagat ccgcaagcga atctctcaca tcaagcagta tcgtaaatcc gtcgttctca
    10921 gcctgaactc gttggccggg tgtgaccggt accgcgtgca ccttgccggc cttgagcgct
    10981 gccattcttg ctgccgtgcc tccctcgata tttaatagac tgtagtcacg gtctttgatc
    11041 aaaccatttc gcttcagcga ttcgacaacg gcaaactctc caatgccgcc gatgccggta
    11101 attcctattg ccttgccctt gagatcgtcc acagtcttca aacccttggc gctgatcagc
    11161 gcgtagctca tcctgctcag atggccgcca atcacgacga caggcgcgcc gcgggcaatg
    11221 ccccgcaaaa tcgcgacgcc gccggcgatg accatttcag cctggccgct catcaataga
    11281 gccatcatga tcgggccact gctggcgagt gtcaccggcc gaacctctaa gccttcgttt
    11341 ctaaagaaac cggcgccacg agcgaccgcc gttggcagcc cggtgctcca actcggcgag
    11401 acgagcgcga cggtgatgac ctgcagttcg gcggcgttcg tgagtttggc agagataaaa
    11461 agcagcgcga gaaaaatgag cagttttcgt ttcattcgag cttcaccttc tacgcactgt
    11521 gctaatgaga taatgtcggc acggtcgttt gctcggccag gcagatcata gttccgagct
    11581 gttccaatag tcaacgcgct gtgtggcctt gacggcatcg ctcgcgctaa cataatgtcg
    11641 ggctgagatt aacgccgcca tgaattttac aaactcataa gccttgggtg atcggaggaa
    11701 aaaatgtccc aaataagaca tatggccaat tgcaccaaca ataaccgtcg ccttgcgcga
    11761 ttctatcaat tgatcttggg aatggaggag gtctggaacg aggaacaaaa cagcccctat
    11821 gctttctacg tcaccgatgg ttacttcaat ctcaactgtt tacagatcca tcaaaccatc
    11881 gccgaagtca aaagagacgt cggcatcaac cactttggtt tcaaggtcga tagcctcaat
    11941 gaaattaaaa ccaagcttag tcagctcgat ccgcccatca gcttgggttc caggccaaac
    12001 gatggccgtt ataccgaggt tcgcattctg gatccggatg gaaacggcgt cgatgtcgcc
    12061 gaacgcggct ggggcacgag agacgacaaa aatcttcccg gagtgagata cgtcggtatc
    12121 aaaacgacta atcccgagcg tctcgccgaa ttctataagt ttatcttctc aatgaaggaa
    12181 gtcgatagga tggataatcc gcgaacggag agcagggcga tctacctttc ggacggcacg
    12241 atcaatctgg ggctgatcat gaatccgccg gtgccgcgcg aaggccttca ggtgttggga
    12301 tttcaagtga agagcattcg ggaggtcgag gagcgattga aacaggcgcg cggcttgacc
    12361 tacaaaggcg aggcgccgct ggcgatcgag ccgagcctct tagcgggtcc ctacaagacc
    12421 gtctcgctaa aagaccctga tggaactcat ttggatttat cggaagaagg ttggaccgtg
    12481 tgattccgcc ggaccaatgt catttttggt cccctaaaac gaggagatgc aacggtgcag
    12541 tgggtcgtct ttaatttaat ctgtgtcatc ggcttctttg gtgccgtctc aacggctgcc
    12601 ggtcaagcgc tcaaggtcaa cgtcagctat accggcgtgg gtccaacgca acttccggtg
    12661 tgggttgcca aagaaaccgg gatctttagc aaaaatggtt tggacgttca gctgattcgc
    12721 gcccaggctg cgacttcggt gatggcgctg ctttcgggag agcttaattt gattcaaggc
    12781 gctgctccgg cagtcgtgca gagtaatctg agaggcttcg gcaccgtcta cgtcgccgcc
    12841 ggttatgtgg gcttcgaata ttggctcatg agccaacgcg atatcaaatc ggcagaacag
    12901 ttgaaaggcg ggctgttagg cgtttccgga cttaccgggg catcggccac agcgacccaa
    12961 ctcgttttga gaaaacttgg actcgtgcct ggtaaagaca cttcggtgat cgtcatcggc
    13021 ggcacgcctg aacgcttgat cgctcttagg agcggaaaaa tacaagcgac tttgctgaat
    13081 ccgcccacga cgttcgtggc tcaaagagaa ggtttcaatg tgttagccga cctgggcacc
    13141 ttaccgtttc agaataatgg cgtagtaacg acccggaagt tcatcaacga aaagcccgaa
    13201 gccgtaagac gatacgtcag gtcacaaatc gaggctgtac acctgatgaa aactgaacga
    13261 gccaccgggc tcaaggtcct ggcgaaatat atgggcggca cgtcggatca agaactgctc
    13321 gagaagagtt acgacgcctc ggtcacggag catacgtttc cggcgaagca gtatccgtca
    13381 caagccggcc tcaagaccgt aattgactcg ctggttgcag acaacccgaa agctaaagag
    13441 gcaaagccag aggatttcat cgacagccaa ttcatcaagg aactcgacga gcgcggcttc
    13501 atcgacaatc tgaaaaacaa aaagtcttaa cggtccttcg ctgtggatct gatcaagaag
    13561 ctgagatccc aagagtccgt gtcgggcttt gtttgtttga acttggtgat gggccccgtt
    13621 gcctgaggtg agaacgaagg ttcgcgggct catggagaat tacggagtcg gggccgccga
    13681 cattgctaat tgagaatata tcgaccctcg atttcgaaga tcctttcaag ggctttgtcc
    13741 aactagtcaa cagcgtcccc agaatacaga ggagggccga ataatggaga atacgaaaac
    13801 cgatccgatt gtcatcgagc aaccagggat gcgcatgacc attgcgcatc gcagcggcct
    13861 cggcggcaac gatgaaggat tgacgtttga tgtcaccacc gtcggttcag agaacgaggg
    13921 caaaagaatc ctgcgcttcg attgctttta caagaaccct cattatcacg tcggcacctc
    13981 cggcggtgaa agcgctgcct acaaaatgaa agaagaaggc atcgaggacg cggttcgttg
    14041 gacgttggag cagctcaaga cccgcttccc cgccatggtc aaacaagccg gctacgaaga
    14101 aatcgctgcg acgatcgatc agcaggcggt taccgatcag ctgagccgtt tagagcgaga
    14161 aatcgtcgcc aaatattagt cgctgttctg gctccgtagg cacagatttc atctgacttg
    14221 ccgagcctct ttgcgtgtgt cttatacgtg ttcatcgcgg agcgctgcct ccggtcggct
    14281 ccgcccgctt aattcacggt tgtgagcggc gattcgaaga ggtgagaact tgttgctgct
    14341 gaccaacaac gacgtggaac aagttttgac gatggctgac tgcgtcgatg tgttgcggcg
    14401 cttctttgaa gaggaaggca agggccatgt gttgacgcgg cagcgtacgg aaagctggct
    14461 gccccacctc acgaccgata cattctatca gtgcaagacg atggaagggg gagttcctta
    14521 catcggcaag tacgtcattc gaatcgattc taacgtaacc agaaaaaagc agctgggtct
    14581 gtcgagccgt gtcgagcatg ttcccttcgg caatgggagc tggatgggca tgctgctggt
    14641 tttttctacc gagacgggcg agcttcttgg ctggatgccc gacggctatc tgcagcgcac
    14701 ccgcgtggga gcgttgtatg cgcttgctgc cgattacctg gcgcgcccgg atgccgaaga
    14761 agtcgggctg atcggctccg gctggcaggc cggcgggcaa attctcggat tgcgttgcgt
    14821 gcgcgacatc aacaaggttc gggtgttcag ttccaatgcc gcgcgccgca gccgattcgc
    14881 cgcagagttg caggcgagct tgggcatcga agtgcgccct atcggcgccg cgcgggatgc
    14941 gttcgccaat gcaaatatcg tcgcactggc gacaaacgcc aacgagaagg ttatggagtc
    15001 ttcctggatc gaacccgggc agcacttgag ttccgtgcgg gttctcgagc tcgatccgcg
    15061 ctcgtacgaa gaatgcgacc gcgtggtggt caaccggatc gagccgtgga ttcaaaatta
    15121 ttatctcggc cagctctgtc cgcaggatat caaggatgcc aaggtcccgc ccgcaccgct
    15181 gcgcgcgatc gaggccggcg aatttttccg gcagcggatc aaaagaaccg gagcgcgcga
    15241 gaaaactttg ttccccaacg aagcgagcaa ttaccaactc ggcgggcagt tcgccgccgt
    15301 ggccggcttc gtcctcgatc gtgcacgcga aaaaggattg ggccgcgacc tgcctgcgga
    15361 atggtttgcc caaacactgg tgcc
//
LOCUS       DASUVS010000462         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_426645, whole genome shotgun sequence.
ACCESSION   DASUVS010000462 DASUVS010000000
VERSION     DASUVS010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426645"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1292
                     /locus_tag="VF182_24270"
     CDS             69..1292
                     /locus_tag="VF182_24270"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX6440255.1"
                     /translation="MSLLVQKYGGSSVATIERIKHVAEKICAAKRTGHDLIVVVSAMA
                     GETNRLLGLAHEISEMPDERERDVLLASGEQVSIALISLALKERGQASRSFLGHQVRI
                     ETDNAYGKARIRNIDSKRILQSLKAGEVVVVAGFQGIDEDDNITTLGRGGSDTTAVAL
                     AAFLNASACEIYTDVEGVFTTDPGICADAQKLPRVSYEEMIELASTGAKVLEIRSVEF
                     AKRFAVPVHVRSSFSDIEGTWVVKEDESMEDLLVSGVSYDKNEVKITILHVPDRPGLA
                     AQVFGPIADAHIVVDMIIQNASADGTTDLTFTVSKADYKKALSIVEKTAPAIAAKGVD
                     VDTDISKVSVVGVGMRTHAGVAAKMFRVLAEEGVNIEMISTSEIKISVVIAAKYTELA
                     VRVLHDAFIGKQGNV"
     gene            1289..>2135
                     /locus_tag="VF182_24275"
     CDS             1289..>2135
                     /locus_tag="VF182_24275"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX6440256.1"
                     /translation="MKDVLIYDTTLRDGCQAEDISFTLEDKLRITEKLEELGVDYIEG
                     GYPGSNTRDADFFKQVKKLNLKNAKIASFGTTRKPSAKAARDLSLKILLSADAPVVTL
                     VGKTWDLHVRDDLRISKKANLEVIADSIAYVKRRVDQVIFDAEHFFDGFRSNPEYALE
                     CLQAAQQGGADWIVLCDTNGGRLPGEIREAISRVNESVTAPLGIHCHNDGELAVANTM
                     AAVEMGARQVQGTINGFGERCGNLNLISLIPNLQLKMGKNCIKPNQLKKLREISHFFY
                     ELANIQ"
BASE COUNT          516 a          516 c          628 g          475 t
ORIGIN      
        1 tcggcgggta actcttagat ttctggtgct gaactcctaa tgtttaaatg attggcggcg
       61 ccttgaatat gtcattgctg gtgcagaaat atggcgggtc ttcagtcgca acgatagagc
      121 gcattaaaca tgtagccgag aaaatctgcg cggccaagcg caccggtcat gaccttatcg
      181 tggtggtctc ggccatggcc ggggaaacca accggctttt agggctggca catgaaattt
      241 cagagatgcc cgatgaacgt gaaagagacg tcttactggc ttctggagag caggtgtcga
      301 ttgccttgat cagcttggcg ttaaaagagc gtggacaggc gtcgcgctcg tttctaggtc
      361 atcaggtgcg aatcgaaacc gataacgcct acggcaaggc gcgcattcgc aacatcgatt
      421 ccaagagaat cttgcaatcg ttgaaggcag gagaagttgt ggttgtggcg ggattccagg
      481 gcatcgatga ggacgacaat atcaccacgc tcgggcgtgg cggatcggac accacggcgg
      541 tagcgctggc ggcgtttctc aatgccagcg catgcgagat ctatacagat gtcgaagggg
      601 ttttcaccac cgacccggga atttgcgcgg atgcccaaaa attgccgcga gtttcctatg
      661 aggaaatgat cgagctagcc agcaccggcg ccaaagtgct ggagatccgt tcagtcgagt
      721 tcgctaagag attcgctgtt ccggtgcacg tccgttctag ttttagcgat atcgaaggga
      781 cgtgggtcgt caaggaggac gagagcatgg aagatttgct ggtctccggc gtaagctacg
      841 ataagaacga ggtaaaaatt accatccttc atgtacccga ccggccgggt cttgcggccc
      901 aggtcttcgg gcccatcgcc gacgcccata tcgtcgttga catgattatt caaaatgcca
      961 gcgccgacgg cacgaccgat ttgactttta cggtttccaa agcggattat aaaaaggctc
     1021 tatccatcgt cgagaagacc gcgcctgcta tagcggccaa aggggttgat gtcgataccg
     1081 atatctccaa ggtctcggtg gtcggcgtgg gtatgcgcac acacgccgga gtggcggcga
     1141 agatgtttcg agttctggcc gaggagggag ttaacattga aatgatttcc acgtcggaaa
     1201 tcaagatctc ggtcgtcatc gcggccaagt acacggagtt ggccgtgcgg gtgctgcacg
     1261 acgcgttcat cggcaaacag gggaatgtat gaaggatgtt ttgatctacg acacaacgct
     1321 gcgggacggg tgtcaagcgg aagatatctc atttactttg gaggacaagc tccggatcac
     1381 ggagaagctc gaagaattgg gagtagacta tatagagggc ggctatcccg gatcgaatac
     1441 ccgagatgcg gactttttca aacaggtaaa gaagctcaat ctcaagaatg ccaagatcgc
     1501 ctccttcggc accactcgca agccgagcgc aaaagcagcc cgggatttga gcctcaaaat
     1561 cctcctgagc gccgatgctc cggtggtcac gttggtggga aaaacttggg acctccatgt
     1621 gcgcgatgat ttaagaatca gtaagaaagc taacttggaa gttatcgccg actccatcgc
     1681 ctacgtgaaa cgccgcgtcg atcaggtgat tttcgatgcc gaacattttt tcgacgggtt
     1741 tcgctcgaat ccggaatatg cgctggaatg tttgcaggcg gctcagcagg gcggcgcgga
     1801 ttggatcgtc ctgtgtgata ccaacggcgg ccgcttgccc ggcgagattc gcgaagccat
     1861 ttctagagtg aacgaatcgg tgacggcacc gctcggaatt cactgccata atgacggcga
     1921 gctcgctgtc gccaacacga tggcggcggt tgaaatgggg gcgcgtcagg ttcaagggac
     1981 tatcaatggc ttcggtgaac ggtgcggcaa tctcaatctg atttcgttga ttcccaatct
     2041 gcaattgaag atgggcaaga attgcatcaa gccaaaccag ttaaagaagc tccgtgagat
     2101 atcgcacttc ttttatgagc tcgcgaatat tcaac
//
LOCUS       DASUVS010000463        32628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_644522, whole genome shotgun sequence.
ACCESSION   DASUVS010000463 DASUVS010000000
VERSION     DASUVS010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 32628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32628
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644522"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VF182_24280"
     CDS             <1..507
                     /locus_tag="VF182_24280"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6440257.1"
                     /translation="AHPGYSGIIMTHVLALVNVYGWDYFRDLAKNKLHIVQSANDPAG
                     VVASGERPVGVNGAEYFYYKTLKQGNPIKIIYPKEGVPLVVSPVAIAKDAPHPNAAKL
                     FTEFIFSKESQQLLADKEGLYTGHPEVTYPADKPKLKDLKLLAADADELEKRNAEIKK
                     RFVEFFGA"
     gene            526..2253
                     /locus_tag="VF182_24285"
     CDS             526..2253
                     /locus_tag="VF182_24285"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6440258.1"
                     /translation="MPVSQPDTLGRLGQTGWSALRFKDPTFPVWIGAALLLVFLMLFP
                     LGAIFRASLWGDTGFTFSRYLEVFTNEQSLTAIWNTLIISTSVGVLAVIIGALLAWLV
                     TRTDLPGKKSIRALVMASFVTPPFLGAFAWTLLAGPNAGALNKLYRAVTGSEEAIFNI
                     FTMPGLIFVMTLYSFPFVFSMIANVCELISSDLEDAAEILGANKWRTAWTVTLPLALP
                     ALIGGFILAFLHSLSLFGAPAILGLPAGLHTITTQIWTLFQYPPRLDMAAAFSVPLLL
                     ATMVLIVAQKKILGRKGYSTVGGKGGQKRLIRLGWGRVPVLFCVLGILSLSVFLPYWI
                     LLKAALSKAWALPLTWDNFTLHNLSFTFFEYGDTQLAIYNTFKLGLITATVGTLLATL
                     IAYITNRNLFRGARYLSFFALAPLVIPGIVLAVGLFIAYTRPPLVLYGTIWILFVAFL
                     TKEMPIGFSQAESTFKSIHPELEDASRIIGANRLTTLKDITAPLARSGLIAAWSFIFI
                     GVIRELSAAILLFAPQSKVVSVVIFDLKEEGQIGVIAVLGILLLLVSFAVIITVQRLA
                     GREVVATRE"
     gene            2257..3351
                     /locus_tag="VF182_24290"
     CDS             2257..3351
                     /locus_tag="VF182_24290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6440259.1"
                     /translation="MAIVSIRGLTKKFTDAAAVDDLHLEIADGEFVSLLGPSGCGKTT
                     TLRLIAGFLQPDAGEICVGGAVISSPSHLVSPERRNMSMIFQSYAVWPHMTVAQNVAY
                     GLKFKKLTKQQIDAKVTKLLGVVHLSELKDRYSSELSGGQQQRVALARALVVEPQILL
                     MDEPLSNLDANLREEMRFEIRRLHEEFGITTIYVTHDQAEAMATSDRIAVLDRGQIIQ
                     VGRPAEIFDRPKTRFVAEFVGKANILSGRLDGGSRMQIGERLVIGVMPNPDLASTTGE
                     AAICLRPHNIVLTAVQSEAETLAREDYNLFSGVVQRRIYFGESADYTIDLAPHPFTLR
                     VVGSPSKLYDKGQRVFALARPEHCVVVSNH"
     gene            3569..4813
                     /locus_tag="VF182_24295"
     CDS             3569..4813
                     /locus_tag="VF182_24295"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6440260.1"
                     /translation="MADYVKEHYDFPLTQKSENKPLKGVRVIDAGNMVAAPFATVLLA
                     DFGADVIKIEHPKYGDGQRKLEPIMSGIPLWWKVVARNKRCITLDLGKPQGAEIFKQL
                     IKGKDVIVENYRPGTFEKWGIGPDILRGIDPRLIMLRISGFGQTGPYKNRAGFGRVAE
                     AMSGLTNLIGEPDGPPMSPGYPLGDLIAGIFGSLSIMMALYHRDLRGGQGQVIDLALF
                     EAVFRFLDFDPIQYDQMKTVHMRTGNRVAYVAPSSMFKTKDGKYLTLAASTQNVWERL
                     ADAIGRKDLITDPKFIDNPARVENSVECNGIVGAWIGQHTRDEVIEQFDKFGVAYSAV
                     FDMEDAFRDLQYRARAAMVRVPDPDLGEAIVQNVVPKFSATPGSVDFLGVKMGAHNEE
                     IYGKELGLSKEQLKELKDAAVI"
     gene            5039..5812
                     /locus_tag="VF182_24300"
     CDS             5039..5812
                     /locus_tag="VF182_24300"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6440261.1"
                     /translation="MNQLFDLLTQGTNLSPAGFLVLCGVSFLGSLIAGALGLGGGVLV
                     LATMANLLSPTVLVPIHGVVQLASNLSRAVLSWRQTLMSIVAPFLFGSIIGAALGAQF
                     VVALPKYLLQIVIAVFILASTWAPKFQSASTGRIKFFIVGVITTIVTMFVGGTGVLVG
                     AFVAPACRGRHQFVSTHAVVMTIQHGLKIVSFALLGFAFGPYVPLLVGLVLCSFIGSY
                     AGKLALNRLPERLFRIALKTTLTILSLQLLYTAVSQLIA"
     gene            complement(6034..7077)
                     /locus_tag="VF182_24305"
     CDS             complement(6034..7077)
                     /locus_tag="VF182_24305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019497364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6440262.1"
                     /translation="MKSALHLVSNENSNPNGSTNHPRMDDTALLDAYSEAVIEAAEKI
                     SPSVVNIEIQHKAADAVRNRSRGPNPLRGSGSGFIFTPDGFILTNSHVIHRADRIEVA
                     LSDGRRNPARLIGDDPDTDLAVIRIDAPNLSAAQLGDSNGIRVGQLAVAVGNPYGFQC
                     TVTAGVVSAMGRSLRSNSGRLIDDVIQTDAALNPGNSGGPLVSSRGEVIGVNTAIILP
                     AQGICFAIAINTAKFVAARLIRDGRVRRSFLGVAGQNVSIPRSLARQLNMATASGVLV
                     LSAEKDSPGQKAGLLQGDVIIAMDGEAIKGVDDLHTHLTDEKIGIKSDLTIIRRSAKI
                     TVPVVPAEIRTVA"
     gene            complement(7955..9133)
                     /locus_tag="VF182_24310"
     CDS             complement(7955..9133)
                     /locus_tag="VF182_24310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEX6440263.1"
                     /translation="MKANCWYSKHDVRVEEVSDPKILNRRDAIVKITSTAICGSDLHL
                     YNGFIPTMEKGDILGHEFMGEVVEVGSEVKNLSTGDRVVASFPISCGSCFFCQKQMFS
                     LCENSNPNAPIAQKLFGHSPAGIYGYSHMLGGFAGGQAEYARVPYADVGTIKIPEGLP
                     DEKVLFLSDIFPTGYMAAENCDIEEGDTVAVWGCGPVGQFAIASAKMLGAERVIGIDR
                     FPERLQMAGEKAGAAETINYEQADVYETLMEITGGMGPDACIDAVGLEAHMPGIIGAY
                     DRVKQAMMLESDRPHALRQAIMACRKGGTVSVPGVYGGFDDKIPLGSFVNKALTLKTG
                     QTHVQRYMKPLLERIEQGEIDPSFVITHRMSLSDAPKGYDIFTNKHDDCIKIVLKPDL
                     "
     gene            complement(9155..10213)
                     /locus_tag="VF182_24315"
     CDS             complement(9155..10213)
                     /locus_tag="VF182_24315"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6440264.1"
                     /translation="MADSDFQESEREQASPILTRQSEYDENRRFQRSGYGDLPRITTN
                     GSRSKSRAGREERLAHALGWFSIGLGLVEIAAPRALAKLIGVHGNRTLLRMLGMREIA
                     SGIGILIRRRPVGWLWSRVGGDIMDLAVLGTALTSPHANRRRIAAASAAVTGVTVLDL
                     RASRQLSSQPELQDNAVSVQVKKSITINRSPEELYQFWHEFQNFPHFMNDLESVQNIG
                     EKRSHWIAKGPAGKRIEWDAEITEDRPNELIAWRSLEGSQVKNSGSVRFEPARGKPGT
                     VLRVEIEYRPPAGLLGVTVAKLLGAEPKQQVHENLHRFRQLMETGEIITTEGQPAGRS
                     QSTSWKHDQAGRRLAASL"
     gene            10759..12363
                     /locus_tag="VF182_24320"
     CDS             10759..12363
                     /locus_tag="VF182_24320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6440265.1"
                     /translation="MRIFLIHIRDPQFYALPATTRAANGNIRIMGFPPIGIMSLSAVL
                     KRAGHECVMFDQANPETPNSIITEEIRRQRPSLIGLSFLSTTSYPYAKVLARQIRAMD
                     SCVRLAFGGVFATLNAEMVKAQCPEVDFICRGDGEQLILDLLERLDDPATVAGLTWAK
                     DGAIIHNPNRAMDRDLDQWPFPDRESLPLDFVESMPLDVPAVLSMERFTTMQTSRGCP
                     WPCVFCDIPIFNEGKWRSRSPQHVIDEFKHLQENGYGAVYFVDDHFLLRPNRIEAICK
                     GINEEGINIQWGCEGRVDSTAQHLFPAMAKANCRTLMFGIESGSQKVLDRLKKDQTLE
                     QVESAVDNAKAAGIEIVHGFFVVGSPDETVEDMRATFEFASRLKLDTFGFNRLCVYRG
                     TPLWKEYVARDLVDDATDWYKYFKCSEIDPTCLSGAVINDERSAGLRRLFRYKVTHYP
                     LQTFRLLRRFLRHMPLRDVVYLIVKPFMGKKQGPTKNEVFSRAVEHRTLKDNAAELTN
                     VPDALLKGVIEESENAATRRPRRVAL"
     gene            complement(12405..13169)
                     /locus_tag="VF182_24325"
     CDS             complement(12405..13169)
                     /locus_tag="VF182_24325"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6440266.1"
                     /translation="MSYQNILTEQIDKVALITLNRPEKLNAMSYELARELDAELTKIE
                     SDDTVSAVVLTGAGPRAFSAGGDIHQMAKSSDQELAARTEFRTQANWRIATFAKPIIG
                     AINGLAYGAGAQLTSMLDIRLGCEITEFRFLAARYGRVNSSWSLPLVVGIAKAKELLY
                     TGRVVQAVEAEKIGLLNQIVPRTELLAKAIDMAQLISENDPRMVQGIKRLLHENIGRD
                     WRGRYDNEQDARKSRLKANHPREGFKEFLSRKGIRN"
     gene            complement(13358..14140)
                     /locus_tag="VF182_24330"
     CDS             complement(13358..14140)
                     /locus_tag="VF182_24330"
                     /inference="COORDINATES: protein motif:HMM:NF004868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEX6440267.1"
                     /translation="MAEQQPKIVTGIGKAELHRILVRGYDLNKELVGKITFTDMTSLM
                     LRGRLPTPNEAKMLDALLVILVEHGMVSHVIAARLVYHCAPEAIQAAVAAALCGAGSV
                     HLGSSEWSAKMLTEALPNESENRDLEQIATNIFDDYSNRKQRIPGIGHRTHAEGDPRA
                     ETLFGIAKENGIYGSYCELLQKLSEVASQRRKRLIPVNVTGAIAAIALDMGFPWQITK
                     AFALIGRTLGVMGHIAEEIRNPMATQIDAAIKQAIVYEPYRT"
     gene            complement(14212..15156)
                     /locus_tag="VF182_24335"
     CDS             complement(14212..15156)
                     /locus_tag="VF182_24335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX6440268.1"
                     /translation="MSTHQRLLIFLIFVLALTSILSPWFAIAADWVTGQVLGVERYPF
                     SRIFNRTFMVSGMVLFFTWRRLLKIGNLTELGFVGLGQGAGDLLRGWSLAVASVAALI
                     LAMSLAQVFEPYLRLSLSRSLSRCFSALLSGVFAGTLEETFFRGILFKGLLQNGRIIR
                     AFVLSSLFYSALHFIRPGEAYFVDSFDPLAGFRHLFYTFTPFSEPLNLLPGMVGLFLL
                     GVVLSYAFFRTGNLYLSIGLHAGWVFGLKLVRVFGDYTRDELGWLFGTTDPKIVSGVA
                     TWIGITSVFLLVHWLTQNRCGLTRERPVRPDAYIPSPR"
     gene            15198..15947
                     /locus_tag="VF182_24340"
     CDS             15198..15947
                     /locus_tag="VF182_24340"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6440269.1"
                     /translation="MTSTPQKKLMKNVALITGATRGIGKAVAAAYVREGARVFICARD
                     RQEVAATVRELRDCSTEAQVDGCAGDVGKETDVCRIVDAAIERFETIDTLVNNASLLG
                     PRVTLVQYPFEQWEEVIRVNLNGVFLLTQQVVKRMIARRRGSIINVSSGVGRTGRARW
                     GAYAVSKFGLEGLTQVLADEVKEFGIRVNSVNPGPTRTEMRAEAYPEEDPLTLPTPEQ
                     ITPIFVHLASVESEDVTGAILEANDWLEHAN"
     gene            16209..17204
                     /locus_tag="VF182_24345"
     CDS             16209..17204
                     /locus_tag="VF182_24345"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440270.1"
                     /translation="MKAIPMVALLVIYALTSVATEVQAQDLKKIKIGYPAVSYNQIHI
                     WVAKEAGLFRKYGLDADVVFFRGGGMATQALVAGDPPIVNIGTVVLAGLQGYDVVLIG
                     SSESTYNYSVVARPVTTKVEQLKGKRLGVSGFGSASHTAALILLKKFNLEPNKDVMLV
                     VAGPTPDRLAAMESNRIDATILTPSETPRARKQGFIEIFDMLDLGIEVQGNGFATSRT
                     FIRNQRETALSALKGYVESIHYIHKNREETKKIVAQYLRTADAEALEATYTAFVKTVA
                     KRPYPTLKGIQYLLDDLAVKNPQAKTVKPEQFVDLSLLQQLEKEGFFAEMAKRYP"
     gene            17254..17754
                     /locus_tag="VF182_24350"
     CDS             17254..17754
                     /locus_tag="VF182_24350"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:17380..17382,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HEX6440271.1"
                     /translation="MNQHIGKQFPDFEMLDHDSQPVKLSQYAGKFPLILSFYRGYWUG
                     KCQVQLRHYVEFQKELVINYCKLVVVSVDPPEVNSAFRTGLGAEFPFLSDQDRKVVQA
                     LEMTETSKSRGVTAIPYTFSLMPDLTVHNFYCGYWYVGRPTLDELRMDLRAMMKKVRT
                     DFDPQA"
     gene            17813..18067
                     /locus_tag="VF182_24355"
     CDS             17813..18067
                     /locus_tag="VF182_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015189605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEX6440272.1"
                     /translation="MITPEQIKSTLEQALPGSTIEMQDLTGGGDHWQVFIVSPAFEGK
                     GLIEQHQMVYAALKDEMGDQRIHALALKTFTPEQWAKLGS"
     gene            18093..18416
                     /gene="grxD"
                     /locus_tag="VF182_24360"
     CDS             18093..18416
                     /gene="grxD"
                     /locus_tag="VF182_24360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017325000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX6440273.1"
                     /translation="MAEDVLKEIDSKVKNNKVMLFMKGTPDFPQCGFSAHTVEILRSY
                     GVPFATADVLADAAVRDGIKRYSNWPTIPQVYIDGKFVGGCDIVHELHERGELEPMLK
                     SALDK"
     gene            18449..18844
                     /locus_tag="VF182_24365"
     CDS             18449..18844
                     /locus_tag="VF182_24365"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6440274.1"
                     /translation="MGNAFAPIILTVAVALLSSCATLRSLTPGRDSFEAGMASFNQGQ
                     FGSAIEHFKQSTSENPNSAQAFLYLGRSYISAGRWRSAIQPLRTAFRLSPHEAKDEIM
                     NLIVDAVFAAATNYPGESGSSGDRYKDLL"
     gene            18919..19644
                     /locus_tag="VF182_24370"
     CDS             18919..19644
                     /locus_tag="VF182_24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HEX6440275.1"
                     /translation="MFKFIAACFFVLAMLTPAWAQFDRVLKGLGGITGAGNGGLNDAK
                     IGSGLQEALKVGTENAVLQTGSIDGFFKNQAIKILMPRPLQNIEQPLRLVGHGPQIDE
                     FVLSMNRAAEKAVPYAKEIFWDAIGQMTFDDASKILNGNDTAATDYFKGKTSNKLHSA
                     FRPTVKTVMADVGVTRQYNDLIGRYKAVPFAQSISFDIDQYVTEKTTDGLFFVVGQEE
                     KKIRTNPAARVTDLLRDVFGSKR"
     gene            20092..21051
                     /locus_tag="VF182_24375"
     CDS             20092..21051
                     /locus_tag="VF182_24375"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6440276.1"
                     /translation="MNSIYKYWKALKHVGVVTVTIVMIVGAIHFPYDIDAPLTEQELE
                     AARKYYTDAYRKPVEENKDKSEYETKYIRVAEAAAKTARIEERVSEFVRQFKLSDREV
                     LEIGSGRGYLQDIAEKYTGLDISPTVARFYHKKFVLGSATAMPFADDTFDGVWSIWVF
                     EHVPNPEQAFREARRVTRDNGVLFLLPAWNCTSWAAEGYEVRPYSDFNTVGKIIKASI
                     PLRSSPPFIAMSKLPNRLVRNLVAQFGPTKLRYRRLSPNYDQYWVPDSDAVNSIDRYE
                     AMLWFRSRGDECLNCDDAPLSLSHMPLIIRVHKSGTELQSRPD"
     gene            complement(21290..22015)
                     /locus_tag="VF182_24380"
     CDS             complement(21290..22015)
                     /locus_tag="VF182_24380"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440277.1"
                     /translation="MYGWRAKIGHVAPSRGDTLVYEFYQMMPKGFMILNSTGTIRQLV
                     DADFERQLQRIEEAAQDLVENKCDAIIIGGSPLFTKLGYGSDIEMEKKLTARFGVPTS
                     PGITGEIAALRSLNIKKLVVATPHEDSLNQRMKAFLEASGFKVLKIQGYGVRKNADLT
                     DMDIHAAYRIAKKLYEQASDADGIFVPCPRWPTITDVDLLEREIGKPVVTSCQAYIWH
                     ALKMAKVKEDVAGFGRLMASLAN"
     gene            complement(22198..22947)
                     /gene="fabG"
                     /locus_tag="VF182_24385"
     CDS             complement(22198..22947)
                     /gene="fabG"
                     /locus_tag="VF182_24385"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012938737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX6440278.1"
                     /translation="MAGIENKVAIITGGGGGIGGAIALRYAREGAKLALADIVADTAK
                     SHAVEINSMGCDALAIITDVTNKKSVQQMVQTTLERWGRIDILVNVAGGADRKSVLEM
                     IEADWDHIIDMNLKSVFLCCQAVLPVMLKQKYGRIVSISSIYGFTGNATRASYAAAKA
                     GVAVFTKSLALEVAKEGISVNAIAPGRISTPRVRGHYSDEQWAEAMTRIPAGRAGTPD
                     DVASTALFLVLDENRYITGQTIHVNGAWLNW"
     gene            23116..24351
                     /locus_tag="VF182_24390"
     CDS             23116..24351
                     /locus_tag="VF182_24390"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6440279.1"
                     /translation="MQQVLEQVDRQRIVEIACKLADTVSITGEEKEVAEYLGSEFERL
                     GMQVEYQEVEEGRPNVIGTLKGSGGGATLMFCAHMDHFDNPQETVVEDDRIYGRGLVN
                     MKAAFPCYIEAIAALQKAGVQLKGDLIISGVVGEIEKAQIGRFQGKNYRGAGVGARYM
                     MDHGVMADMCIIGEPTGLQLQIGNAGLLWARISVDGRATKFVLAACKVAQAIQDWEKE
                     YQKKNPHKFMLPTVQIGAIDGGNAFKPGTRPTTDIFVIVKMLPGAVPLAIKRDIEQLC
                     ERVRKREPDILDIRVDLYLTTPGYEISKREYVVKAMEQAHRTVFNQPVPYAKPIRYGI
                     TSDGSRIAAYGVPAITYGPGFGTHLVDPTETKAGPEWDAPSGVRRGVGIENMINCTKV
                     YAMAALDIVNQKKDDVAKT"
     gene            24362..25243
                     /locus_tag="VF182_24395"
     CDS             24362..25243
                     /locus_tag="VF182_24395"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440280.1"
                     /translation="MQITFAHYRNRFCMYRTYYALAKGIVKPDGFTMKVIELPDPPSH
                     EQEDALIRGEVQVANLYLPNFLRRKLAGAPIVGLCTEWKSTMKGNGMFVRKDGPVHDP
                     RDLAGRVIGSHQGPHAIHRYLLRHKFGIDDAGLQWVSYPQEQLLEAMKRGDADAVVLL
                     DQFFFHGEQDPEVQCLYTDGEIWHSLLGFSEIIKHMIAMEETLCREQPELKEKLQAAF
                     RASFAYSEEHLEEIAGEFVKKYPGDKQALLASARYPKIEFTLTETEQKLADAEMDMMV
                     EVGWIPRKAPVQSLFAI"
     gene            25284..26261
                     /locus_tag="VF182_24400"
     CDS             25284..26261
                     /locus_tag="VF182_24400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440281.1"
                     /translation="MLRPRNFLYSFERAVASIIFIFLASSCTLMGVPIRNETELLRDA
                     SATIQEIKAFEKDLGIEPTQALAQTSEQKTPTSMLWFWLQRAGTLALRTPIDVRLTLG
                     FAKSKEEVALDQVYRVDGYSVYYRQGNEFADPRSVTTKSFARAGAVRQVAVVIHEDLH
                     GEANFDLPWEIEESLITPLGSVAAVEFFKRKSDSANLESAQERLDEESQLSRELGQLV
                     TVAETIFSNQSVDSAKEKIVAMIPAYPVYHRYFQRQIQGQHPETALEAKLSHDLVYYR
                     YFDQIVALYETTGDLKALVRDLRKAPRDAQALAAYLRSLEKSYAIANCD"
     gene            26488..27102
                     /locus_tag="VF182_24405"
     CDS             26488..27102
                     /locus_tag="VF182_24405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440282.1"
                     /translation="MRIFNFFVLAAMLCFGGCASTPMLVSQWSNPAYESPYFKKIAVS
                     CVDGHISIRRNCEDEFVARLRAAGVNALPSYRNFSEDAKLDGAKLKQMAKEAGADAAL
                     IVRSVGVEEKTEVSPTYFPAPGFGIFGRNISATWYGWYGAPRVYRYEEYTSEATLNDL
                     IKNEVVWTGTLKTTASDNVDSAIKTYVDNVIKALNDRNLLGAKQ"
     gene            27442..28395
                     /locus_tag="VF182_24410"
     CDS             27442..28395
                     /locus_tag="VF182_24410"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6440283.1"
                     /translation="MKKILVGYDGSEHADAAIDDLRRAGLLQDVEAIVLTVADAFVPP
                     AVDAKDWGPSPLDEKIRKRSEEMRARALKARDEAASVAAKGAARIKELFPSWAVRAET
                     DVNWPGWGIILKAEEWHADMVVVGAGKHSFIERLQMGSILRKVVGACERSVRVGRPSS
                     RAQETSVRIIIGLDGSPDAELAMEAVAERVWPAGSEAHLMTVLDNRLSILTPSLIPRL
                     ARWSSAADSVDDQAWVERMMKAATDKLQTAGLKISCEVVKGAPSQRLLDAAVDWQADC
                     IVMGARGLTGIERFLIGSVSSDVAVRANCSVEVVRSTRSRP"
     gene            28564..28785
                     /gene="rpoZ"
                     /locus_tag="VF182_24415"
     CDS             28564..28785
                     /gene="rpoZ"
                     /locus_tag="VF182_24415"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX6440284.1"
                     /translation="MARITVEDCLSEIPSRFELVILAAKRAKQLLKGAKPLLASDNRA
                     IVVALREIAAGKVRRAAPEPEALPAPERT"
     gene            complement(29117..30121)
                     /locus_tag="VF182_24420"
     CDS             complement(29117..30121)
                     /locus_tag="VF182_24420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX6440285.1"
                     /translation="MANLPRIALILTGGTIDSLGKDRLDLAWYIEAGKRLGDGELLQQ
                     LPELKDIAQVQEIPFRRLPSHALVDKDWLDLVRTIHSIFDDNKADGIVITHGTNTIEE
                     TAYFLNLTLKTDKPVVVVGSMRPASAISADGYLNLVNAIKVSAEPSSRGRGCMLVMND
                     TIFNGRDVTKNATYRVEAFQSRDLGPLGFADGDGKIVYYHQPVRKHTVDTEFDVRNLQ
                     TLPRVDIVLSYVGADGTMIEAAADAGAKGIVSAGTGAGRPTPAEDAAFDKAFKEKGML
                     MCLCSRVASGRVVRSPGLAKRGFVAGDNLQPWKARLLLSLALTKTNNADEIQRMFDTY
                     "
     gene            complement(30320..31252)
                     /locus_tag="VF182_24425"
     CDS             complement(30320..31252)
                     /locus_tag="VF182_24425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX6440286.1"
                     /translation="MKYGVFLPVSGRAASRKTLMQAAQQAESLGYDSVWAADRLVIPW
                     KIETAYPYSKESTFIVPPDRPFFDTMTCLAFLAGCTEKIQLGMSVMVLPYRHPLHWAK
                     IATTIDQLSTGRLIMGVGVGWMEEEFAAMNAPFKERGTVSDEQLALLKQLWTEEHITF
                     KGKYYNVDDIAFNPKPFQKPRVPIWVGGEGKYAQRRAGRYGDAWFPYFVRITPLELAA
                     RFQYVRKIARESGRRPDELVLACCLPIELTAKDSHQEEDYLKGSIEQISDRLKMFQKV
                     GVTHIGLQFMIPHYPERQQQIEHFARDALPALRS"
     gene            complement(31442..32305)
                     /locus_tag="VF182_24430"
     CDS             complement(31442..32305)
                     /locus_tag="VF182_24430"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6440287.1"
                     /translation="MSPELLAWLTAVSFALSNVSVRDGLNYSTPFTATLVSLIIHTVA
                     LWIVVFLTMGIPRVALAAVVAIVVTGILQPIMRHCHYTGIHKLGTSRAVTLRNTFPLP
                     AVLIGVVFLGEPITPLGIAGTLSVVAGIVLTSWRIDKHLPSFHWTHLMYPIATALITA
                     VVHPLRRYALIQSHEPLFFTALVGPVSLIALAAYYLTPICKEKLVWDRRAFWPFFVSG
                     FFETLAVLLMLLAFSLGPVVVVSPIASTTPIWTLIISAIFLRQVERITIASAVGTLLV
                     VAGVVAIALTH"
BASE COUNT         7404 a         8612 c         9010 g         7602 t
ORIGIN      
        1 gctcatcccg gctacagcgg gattattatg acgcatgtgc tggcgctggt gaacgtctat
       61 ggctgggact attttcgcga tcttgcgaaa aacaaattgc atatcgttca atcagctaat
      121 gatccggcgg gcgtcgtcgc atcgggagag cggccagtag gtgtgaacgg ggcagaatat
      181 ttctattaca agacattgaa acagggcaat ccgatcaaga tcatctatcc caaagaggga
      241 gttccgttgg tggtttcacc ggtagctatt gccaaggatg cgcctcatcc caatgcggcg
      301 aaactcttca ccgaattcat tttttctaaa gagtcgcagc agcttttggc tgataaagaa
      361 ggtctgtata ccggccatcc cgaggtgacc tatccggcgg acaagcccaa gctcaaggat
      421 ctaaagctgc tggcggccga cgctgacgaa ctggaaaaac gcaatgccga gataaagaaa
      481 cgcttcgttg aatttttcgg cgcataacag cggggagaaa tattcatgcc cgtttctcaa
      541 cccgataccc ttggtcggct cggccagacc ggctggtccg cgttacgatt taaagatccg
      601 acgtttccgg tttggatcgg cgcggcgctt cttcttgttt tcctcatgct gttccccttg
      661 ggcgcgattt ttcgcgccag tttgtggggt gataccggct tcacattcag caggtatctt
      721 gaagtcttca ccaacgaaca atctctcact gcgatctgga acacgttgat catttcaacc
      781 tcggtcgggg tgctcgcagt gatcataggc gctttgctcg catggttggt gacacgcacg
      841 gacctacccg ggaaaaagtc gatccgcgcg ctcgtcatgg cctcgttcgt gaccccgccg
      901 ttcctgggag cgtttgcctg gacgctgctc gccggaccca acgcaggtgc gctgaacaag
      961 ctctatcgcg ccgtcaccgg ctccgaggag gcgatcttta acatatttac gatgcccggg
     1021 ctgatttttg tcatgacgct ctacagtttt cccttcgttt tttcgatgat cgccaatgtg
     1081 tgcgagctga tctcgtctga tctcgaagat gccgctgaga ttctcggagc gaacaaatgg
     1141 cgcaccgcat ggaccgttac cttgccgctg gcgctgccag cgctgatcgg cggcttcatc
     1201 cttgcttttc ttcattcgct gtcgctcttt ggcgcgccgg ccatactcgg cctgccggcg
     1261 ggcttgcaca cgataaccac acagatctgg acgctttttc aatacccgcc gcgcctggac
     1321 atggccgcgg ctttttccgt gccgctgctt ttggcgacga tggtgctgat tgtcgcgcaa
     1381 aaaaaaattc ttgggcgcaa aggctacagt accgtcggcg gcaaaggcgg acagaaacgg
     1441 ctgatccgcc tgggctgggg ccgggttccc gtgctgtttt gcgtgctggg aattctttcg
     1501 ctttcggttt tcttgcccta ttggatattg ctcaaggcgg cgctttccaa agcctgggcg
     1561 ctgcctttaa cgtgggataa ttttacgctc cacaatcttt cgtttacttt tttcgagtat
     1621 ggcgatactc agctggcgat ctacaatact tttaagttgg gactgatcac ggccaccgtc
     1681 ggcaccctat tggccacgct tatcgcttac attaccaacc gcaatctgtt tcgtggcgcg
     1741 cgctatttga gtttttttgc gctagcgccg ctggtcatcc caggcatcgt tctagctgtg
     1801 ggtctgttca tcgcctacac gcgcccgccg ctggtcctct acggcacgat atggattctt
     1861 ttcgtggctt ttttaaccaa ggaaatgccc atcggatttt cccaggcgga gtctaccttc
     1921 aagagcattc accccgagct cgaagatgcc agccggatta tcggcgcaaa ccggctgacc
     1981 acgcttaagg atattaccgc gccgctggcg cgctcgggat tgatcgccgc ctggtcattt
     2041 atctttatcg gcgtcatccg cgaactgagc gcagccattc tcttattcgc cccgcagtcg
     2101 aaagttgttt ccgtggttat cttcgacctc aaggaagagg gtcagatagg agtcattgcc
     2161 gttttaggaa ttctcttgct gttggtttcc ttcgcggtga tcatcaccgt ccagcggttg
     2221 gcgggacggg aggtggtggc gacgcgggaa tagttcatgg cgatcgtctc aatacgagga
     2281 ctcactaaaa aatttaccga cgcagccgcc gtcgacgatc tccatttgga aatcgccgac
     2341 ggcgaattcg tctcactgct cggtccctcc ggttgcggca aaaccacgac gcttcggtta
     2401 atcgccggat ttctgcagcc cgatgctggg gaaatttgtg ttggcggtgc agtcatctcg
     2461 tcgccatccc acctcgtgtc gccggagcgg cgcaacatgt cgatgatctt tcaaagctac
     2521 gccgtctggc cgcacatgac ggtggcgcaa aacgttgcct acggactcaa atttaaaaaa
     2581 ctcaccaagc agcagatcga cgcgaaagtc accaaattgc tcggcgtcgt gcacttgtcg
     2641 gagttgaaag accgctattc gtccgagctc tccggcgggc agcaacagcg tgtggcgctg
     2701 gcgcgggcgc tggtggttga gccgcaaatt ttgctcatgg acgaaccgtt gagcaatttg
     2761 gatgcaaacc tacgtgaaga gatgcgcttc gagatcaggc ggctgcacga agaattcggt
     2821 ataaccacga tctacgtgac tcatgatcaa gccgaggcca tggccacatc cgatcgcatc
     2881 gccgtgctcg accgcggtca aataatccag gtcgggcgac cggctgaaat cttcgatcgt
     2941 cccaaaaccc gctttgtggc ggaattcgtc ggcaaagcta atattctctc tggtcgactg
     3001 gatggtggca gtcgaatgca gataggcgag cgactggtaa ttggcgtaat gcccaacccg
     3061 gatctcgcct cgacgacggg agaagccgcg atttgtttgc gtcctcacaa cattgtgctg
     3121 acggccgtgc agtccgaagc agagacgctt gcgcgcgagg attataatct gttctcgggt
     3181 gtggtacagc gcagaatata cttcggcgag agcgcggact acaccatcga tctcgctccg
     3241 catcctttca cgcttcgggt cgtcggctcg ccgtctaaac tttatgacaa gggccaaagg
     3301 gtattcgcac tggcccggcc cgaacactgt gttgttgtaa gtaaccattg agctaagcga
     3361 gcagagacaa tccagagtgc ggttttccat tctcttgatc caattatttc cggctgttat
     3421 acgctacaat acgtcaccta ggcgattcga gtcctgccca gcatcatcgc ggcccggcta
     3481 tttagcagcg gtaatataag tgcattccgc gcggaccggt taacctgagg taaatcctag
     3541 gatcagtttt gacagtgagg cataccaaat ggctgactat gtaaaagagc attacgactt
     3601 tcccctgact cagaagagcg agaacaagcc gctaaaaggc gtgcgcgtga tcgatgcggg
     3661 caacatggtt gcggcgccgt tcgccaccgt cttgctcgcc gactttggcg ccgatgtcat
     3721 taagatcgag catcccaaat acggcgatgg ccagcgcaag ctcgaaccga tcatgagcgg
     3781 gattccgcta tggtggaagg tggtggcgcg caacaaacgc tgcatcaccc tggacctagg
     3841 caagccgcaa ggcgcggaaa tcttcaagca actgataaaa ggcaaggatg tcattgtcga
     3901 aaattatcgg cccgggactt tcgagaagtg ggggatcggt ccggatatcc tccgcggcat
     3961 cgacccgcgc cttattatgc tgcgcatctc cggattcggc cagacggggc cgtacaaaaa
     4021 tcgcgccgga ttcggccgag tcgccgaggc gatgagcggc ttgaccaatt tgattggcga
     4081 gccggacgga ccgccgatgt cgccgggcta tccgcttggc gatttaatcg ctgggatctt
     4141 cggatcgttg tcgatcatga tggcgcttta tcatcgcgat ctccgcggcg gccaaggcca
     4201 agtgatcgat ctcgcgcttt tcgaagcggt ctttcgcttt ctggatttcg atccgattca
     4261 gtacgaccag atgaagacgg tgcacatgcg cactggcaac cgagtcgcct atgttgcgcc
     4321 gagctcgatg ttcaagacca aagatggcaa atatctgacc ctagcggcga gcacgcaaaa
     4381 tgtctgggag cgtcttgccg atgccatcgg acgcaaggat ctgatcaccg accccaagtt
     4441 catcgacaat ccggcgcggg tggaaaactc ggtcgaatgc aacggcatcg tcggtgcgtg
     4501 gatcggacag cacacgcgcg acgaggttat cgagcaattc gataaatttg gggttgccta
     4561 ttcggctgtc ttcgacatgg aggatgcatt ccgtgacctc caatatcgcg cccgagcagc
     4621 tatggtccgg gtgccggacc cggatctagg cgaagcgatt gtgcagaacg tcgtgccaaa
     4681 gttttctgcg acgccgggtt cagtagactt cctcggcgtg aagatgggcg cacacaacga
     4741 agaaatttac ggcaaagagc ttggcctgtc caaagagcag ttgaaggaac tgaaagatgc
     4801 agcggttatt tgatgctcga tccgcagcag gcggatcgac gaagataacc ggtccttgta
     4861 aatggacgat cgtctatgcg aatcgcggtg gcttgggggc tgttcggttg gataggtatt
     4921 ccactctcat gatttgctga tactcagttc gccaaaactt gcgttagaga ttcagccggc
     4981 ggtttggctt tgcgctgcgc agccaattac cgcgccgcag agcgcgtcat tagaaatagt
     5041 gaatcaactc ttcgacctcc tcacccaggg taccaatctt tcgccggcgg ggtttctagt
     5101 tctttgcggg gtgtctttct tgggaagcct gattgccggt gcgctcggcc tgggcggcgg
     5161 cgtcttggtt cttgcaacta tggcgaatct cttgtcgccg actgtattgg tgccgattca
     5221 cggcgtggtg cagcttgctt cgaacttgag ccgcgccgtt ttaagctggc gtcaaacttt
     5281 gatgtcgatt gtggcgccgt ttttattcgg ctcgatcatc ggcgccgcgc tcggagctca
     5341 atttgtcgtc gccctaccga agtatttgct gcagatcgtc atcgcggtct ttattcttgc
     5401 ctcgacctgg gcgcccaagt tccaaagtgc ttccaccggc aggattaaat ttttcatcgt
     5461 tggcgtgatc accacgatcg tgacgatgtt cgtgggcgga accggtgtgt tggtgggagc
     5521 gtttgtcgct cccgcatgcc ggggtcgcca ccagttcgtc agcactcacg cggtggtcat
     5581 gacgattcag catggtctta agatcgtgag ttttgcgctg ctcggattcg ccttcggtcc
     5641 ttatgtcccg ctgctggtcg ggttggttct gtgcagtttt atcggtagct atgccggtaa
     5701 gctggcgctc aatcgcctgc cggagcggct ctttcgcatc gccctcaaga cgactctgac
     5761 gattctcagc ttgcagctgc tctataccgc ggtcagtcaa cttatcgcgt gatgaattga
     5821 ttgaagagca ggtgcaactt ataaccggca agcatgtttg ctgatgccag acccgcgccc
     5881 gcattcgtgt tgggcgataa cgcctatgag attgatgtac tggccaacca aaagatattt
     5941 accgccgagg cgcggagaat gccgactctc gtctctgcgc tctctgggcc tcggcggtta
     6001 gtctctgctg gacgcactct aagtgtagaa tgctcaagct accgttcgga tttctgcggg
     6061 aacgaccggc accgtgattt tcgcggaacg ccggatgatc gtcagatcgc ttttgatgcc
     6121 gatcttttcg tcggtcaagt gcgtgtgtaa atcgtcgacg cctttaatgg cttcgccgtc
     6181 catcgcaata atcacgtcgc cctgcagcag tcctgctttt tgaccggggc tatctttctc
     6241 ggccgagaga accagcacgc cgctggccgt cgccatgttt aactggcgcg cgagggaacg
     6301 cggaattgag acgttttgac cggcgactcc caaaaaactc cgccgtaccc gcccatcgcg
     6361 aatcaaacgc gcggcaacaa actttgcggt gttgatcgca atggcaaagc agattccctg
     6421 ggccggcaga atgatcgctg tattgacgcc gatcacttct ccccgggagc tcactagcgg
     6481 cccgccggaa ttgcctgggt taagggcggc gtcggtctgg atcacatcgt cgatcagccg
     6541 tcccgaattc gaacgcagcg atcgccccat cgcgctcacg accccggccg tgacggtgca
     6601 ttggaagccg taaggattgc ccacggcgac ggcaagctgg ccgactcgta tgccgttgga
     6661 atcgccgagt tgggcagccg aaagattcgg cgcgtcgatt cggatcacgg ccaaatcggt
     6721 atccggatca tcaccgatta gacgcgccgg attgcgccgc ccgtccgaga gcgctacttc
     6781 gatgcgatcc gcacgatgaa tcacgtggct gttggtcagg atgaatccat ccggcgtaaa
     6841 gataaaaccc gatccgctcc ctctcagcgg attcggaccg cgcgaacgat ttcgcaccgc
     6901 atcagcggcc ttatgttgga tctctatatt gactaccgac gggcttattt tctccgctgc
     6961 ttcgatcact gcttccgagt aggcatcgag caaagcggta tcatccatgc gcggatgatt
     7021 tgttgaacca ttcgggttgg aattttcatt ggacacaaga tgcaatgcag atttcatggc
     7081 ttcctttcgt ttacgaacaa gcaacagaat ttaagaactc aaaggagctt tgtaaaggcc
     7141 gacgtgagtg acagatcgag ttaggtcagg cctgcgggaa tgctcgctga cctcgccggt
     7201 ttcgcgatag ctccgacagt tctcggtgcg aacagcaggg ggtgtctgcg gctcaaattg
     7261 aagatcagtt tgagcgatga agctcgtcgg aaaccgattt cgatgtgaga ttcgcgctgc
     7321 cgcagggtca tcacgggtgt ctttcagtgc ttccacgtat tgtggatctc gcgtcatttg
     7381 atgtcgattt gtgggacttt gatgacactt ttcttgtgag cggatctttt tcttgacgct
     7441 tgcccttata tggggtacaa acaccgccag cttttttgag gattcgaagg agtcgtttga
     7501 tgtcgcgaaa aattgctcga atctgcccgc gctgcaatgg ttatttggga gttactttag
     7561 gagagcggtc gcgtaagtcg atgactcagt cagttaatgg atcatgcctt gtctgcagcc
     7621 ataagctaac ctggacctta gtccatggaa cgcgtcccag cacagccgat aactccggcc
     7681 ggattccgaa attgtttgac cgattgacaa ataaccctcg cattgtcccc accaataatc
     7741 agtgagtttt acgccaacga gaactgtttc ggcgctaact ttacaaagtg cgcgcgggta
     7801 gcatcgcact cggaattccg tcctttgggt gtgacgggct atgccctgcc ctgaggagtt
     7861 agagtcggag aactggttga cgatgtcagc cgcatcgccg tccggtttta gattgctcga
     7921 ttggaagttg ccatcgcgcc ccgttgaaga ccgcctacaa gtccggtttc aaaacaattt
     7981 taatgcagtc atcgtgctta ttggtgaaga tgtcgtaacc tttcggcgca tcactgagcg
     8041 acatccgatg agttatgaca aaactcggat cgatctcgcc ttgttcaatc cgttcaagca
     8101 acggtttcat gtaacgttga acatgcgtct gtccggtttt taacgtcagt gctttattca
     8161 cgaaagaccc gagggggatt ttatcgtcga agcctccata gacgccggga accgataccg
     8221 tgccgccctt gcggcatgcc atgatcgcct ggcgcagagc gtgtgggcga tcgctttcca
     8281 gcatcattgc ttgtttgacg cggtcatagg cgccgattat gccgggcata tgtgcttcca
     8341 acccgaccgc atcgatgcag gcgtcgggtc ccatgcctcc agttatttcc atcaaggtct
     8401 catacacatc agcctgctca tagttgatag tttctgcagc gccggccttt tcaccagcca
     8461 tctggagccg ctcgggaaac cgatcaatgc cgatcactcg ctcggcgcca agcatcttgg
     8521 cgcttgcgat ggcaaattga cctacaggac cgcaacccca caccgcgacg gtatcgcctt
     8581 cctcgatgtc gcagttttcc gccgccatat atccggtggg aaaaatatcg gagagaaaca
     8641 aaaccttttc atccggcaat ccttcgggaa tcttgatcgt gccgacgtcg gcatatggca
     8701 cacgcgcgta ttccgcctgc ccgccagcaa agccgccgag catatgcgag tagccgtaaa
     8761 taccggcggg ggagtggcca aatagttttt gcgcaatcgg ggcattcggg ttggaatttt
     8821 cgcacaaaga gaacatctgt ttttgacaga aaaagcagct gccgcaggag attggaaacg
     8881 aagccacaac ccgatcaccg gtgctcaagt ttttgacttc gctgccgacc tcaaccacct
     8941 cgcccatgaa ctcgtggccg aggatatcgc ccttttccat cgtcggaatg aatccgttgt
     9001 acagatgcaa gtcggagccg caaatcgctg tcgaggttat ctttacgatg gcgtcacggc
     9061 ggttaaggat ctttggatcg ctcacttctt caacccgcac atcgtgcttg ctgtaccagc
     9121 aattagcttt catcggatcc tcctgttaga tgggctaaag cgaagcggcg agacggcgtc
     9181 cggcttggtc atgtttccag gaggtgcttt gggagcgtcc cgctggctgg ccttcggtgg
     9241 taatgatctc gccggtttcc atcagctgtc tgaatcgatg caagttctca tgcacctgct
     9301 gtttaggttc tgcccctagt aatttggcaa ctgtcacgcc gagcaagccg gcaggcggac
     9361 ggtactctat ctcgacacga agcaccgttc ctggtttccc ccgtgccggc tcaaaccgta
     9421 ccgatcccga gtttttcacc tgagaacctt ctagcgagcg ccaggcgatt agctcattgg
     9481 gccgatcttc ggtaatttcg gcgtcccact cgattcgttt gccggccggg cccttggcga
     9541 tccagtgcga acgtttttct ccaatgttct gtacggactc tagatcattc atgaaatgtg
     9601 ggaaattctg aaattcatgc caaaattgat aaagctcttc gggagatcgg ttgatggtga
     9661 tgctcttttt tacctgaaca gaaacggcat tgtcctgaag ttccggctgg ctgctgagtt
     9721 gccggctcgc gcgtaggtca agaaccgtga cgccagtaac tgcggcagaa gccgccgcga
     9781 ttctgcggcg atttgcgtga ggcgaagtaa gagcggtgcc cagaaccgct aagtccatga
     9841 tgtcgccgcc aacccgtgac caaagccagc ctactggcct ccgacggata aggatgccga
     9901 tgccgctcgc tatttcgcgc attcccagca tacgcagcaa cgtgcggtta ccgtggacac
     9961 ctattagctt cgctagcgct cggggagccg cgatctcgac aaggcccagg ccgatgctga
    10021 accatccaag cgcgtgcgca agtctttcct cccgtcccgc tctcgatttg cttcggctgc
    10081 cgttagtggt gatgcgagga agatcgccat aaccgctccg ttgaaacctt ctattctcgt
    10141 cgtattcact ttgccgtgtt aggatcggcg atgcttgctc gcgttcggat tcttgaaaat
    10201 cggaatcagc catggttttc tccagataaa aattaaccaa gccagtacag tactccgcga
    10261 actcgggagc tagaatgtcg gtgcaagtga tgctttggga caaaaatcac ttgcgcaaat
    10321 catgtgccat cgacagcttc ttttagctca aagctgaact cgagagtgca attggaatag
    10381 cccggcagcc agcgcaaagg ctgcggcgaa tataggacaa tcagttagag atgacttgcc
    10441 aattgtggga cgaacgacga gtacatactg ttgcggcatc cgagcaaatt aagctcgagc
    10501 agcagcaaca cagaattatc cagatctgat cgtgcaatga agcgcgtctt tgtaccgtta
    10561 cctgtggcta gaatcgtaca gctgaaatct tgttcgttcg gaatttggtt gttaactcgg
    10621 ccatgctttg ctatggttta ctaggtcagg ctgactttca tgcactagtg ttatggacta
    10681 gtgttagaac cctacgatcg tctctcccga gtgtggttcc aaccttaggc tgtcacggcc
    10741 tgtcgaaatg agtactcaat gcgcatattt ctgattcaca ttcgcgatcc acagttctat
    10801 gccctgccgg ccacgacgcg cgcggccaac ggtaacatca gaatcatggg ttttccgccg
    10861 attggtatca tgtccctttc ggccgtgctg aaacgcgctg gacacgagtg cgttatgttt
    10921 gatcaagcca acccggaaac gcctaactcg atcattacag aggagattcg ccggcagcgg
    10981 ccgagtctta tcgggttgag cttcttgagc acgaccagct atccgtacgc taaggttctt
    11041 gcgcggcaga ttcgtgccat ggattcatgt gttcgattgg catttggcgg cgtgttcgcg
    11101 accttgaatg cggaaatggt caaggcgcag tgtcccgagg tagattttat ttgccggggc
    11161 gatggcgaac agttgatcct cgatttgctt gaacgtttgg acgatcccgc cacggttgcc
    11221 gggctcacct gggcgaaaga cggcgctatc atccataatc caaaccgcgc aatggaccgc
    11281 gatcttgatc aatggccttt tcctgatcgc gagagcttgc cgctcgactt tgtcgaatcg
    11341 atgccgcttg atgtcccggc ggtcctatcg atggagcgct tcacgacgat gcaaacctca
    11401 cggggctgcc catggccttg tgtcttctgt gatatcccaa ttttcaacga aggtaagtgg
    11461 cgctcgcgca gcccgcagca cgtcattgat gaatttaagc atttgcaaga aaatggctac
    11521 ggcgccgtat atttcgtcga cgaccatttt ctcctgcgcc ctaatcggat cgaagcgatc
    11581 tgcaaaggga ttaatgagga agggatcaac atccagtggg ggtgcgaagg gcgggtcgac
    11641 tcgactgctc agcatttgtt ccccgccatg gctaaagcca attgccgcac ccttatgttc
    11701 ggtattgaaa gcgggagcca aaaggttttg gaccggctaa agaaagatca gactctggag
    11761 caggtagaaa gcgcggtcga caatgccaaa gcagcaggca tcgagattgt tcacggtttc
    11821 tttgtcgtcg gcagtcccga cgagaccgtt gaagacatgc gcgcgacctt cgaatttgct
    11881 tcgcgattga aactcgatac ctttggtttc aatcgtctgt gcgtttaccg tggcacgccg
    11941 ctttggaagg agtatgtggc gcgcgatcta gttgacgatg ctaccgactg gtataaatat
    12001 tttaaatgtt ccgagatcga tcctacctgc ctgtcgggag cagtcatcaa cgacgagcgc
    12061 agcgccgggt tgcgtcggct gtttcgctac aaggtgacgc actacccgct gcaaaccttc
    12121 cgactcttac gccgcttttt gcgacatatg ccgctgcgcg acgttgtcta tttaatcgtc
    12181 aaaccgttca tgggaaagaa gcagggtccg accaagaacg aggtgttttc gcgcgccgtc
    12241 gaacaccgca cgctcaaaga caatgccgcg gagctcacga atgtccccga tgcgctgctc
    12301 aaaggggtca ttgaagaaag cgaaaacgcg gcgactcgcc ggcctcggcg agtcgctctc
    12361 taagttaacg agagaagcct gcgccagctt ttactgcatg cttgttaatt gcgaatccct
    12421 ttacggctga gaaactcctt gaacccctcg cgcgggtgat tcgctttgag ccggctcttt
    12481 cgggcatctt gctcattgtc gtagcgcccg cgccaatccc tgccaatatt ctcgtgcagc
    12541 agccgtttga ttccctgcac catgcgggga tcgttttcac taatcagttg agccatgtca
    12601 atcgctttag cgagcagttc cgtgcggggc acgatctgat tgagcagccc gatcttctca
    12661 gcctcaacgg cctgaaccac tcgtccggtg tagagcagct ctttcgcttt ggcgataccc
    12721 accaccagcg gcagcgacca agatgaattg acccgtccgt agcgcgctgc caggaagcga
    12781 aattcggtga tctcacaacc gagccggatg tcgagcatgg aggtgagttg ggctccggcg
    12841 ccgtaagcca aaccattgat ggcgccgatg atcggcttgg cgaaagtagc gatacgccag
    12901 ttggcctgag tgcggaattc ggtccgggcg gcaagctctt gatcgcttga tttggccatc
    12961 tgatgaatat cgccgccggc ggagaatgcg cgtggcccgg cgcccgtcaa aaccacggcg
    13021 ctaacagtat catcgctctc aatcttcgtc agctcggcat cgagctcccg ggccagttcg
    13081 tagctcatgg catttagttt ttccggacgg ttgagagtga tcagagcgac cttatcgatc
    13141 tgttctgtca agatgttctg gtaactcatc gggcctcctt caggcacagt gaattgtcat
    13201 cgtcggcgcc gcgattacca caaaagccga aagctgcgca atgtttcggc ggcatccagc
    13261 gcggttcggc ttaccggcaa ttctttgcgc aagagcgata gtcgtctgcg cagcaggatt
    13321 aaccgacgaa aatgatcccg gctgtatagt tctggcctca ggttctataa ggttcgtaaa
    13381 cgatggcctg tttgattgcg gcatcgattt gcgtcgccat cgggttccga atctcctcgg
    13441 caatgtggcc catcacgcca agcgtgcggc cgatcaacgc gaaggccttg gtgatttgcc
    13501 aaggaaagcc catgtccaga gcaatcgccg cgatggcgcc ggtgacattg accgggatta
    13561 accgtttcct tcgttggctg gccacttcgg aaagcttttg cagcagctcg caatagcttc
    13621 cataaattcc attttccttg gcgattccga agagggtctc ggcgcgagga tcgccttcgg
    13681 catgcgtgcg atggccaatc cccggaatgc gttgcttccg gttagagtag tcatcgaaga
    13741 tattcgtcgc gatctgttcg agatcacggt tctcgctctc gtttggcagc gcctcggtaa
    13801 gcattttggc tgaccattcc gacgatccaa gatggacgct gccggcgccg caaagcgccg
    13861 cggcgacggc agcttgaatc gcctcagggg cgcaatgata aacgagccgg gcggcaatga
    13921 catgcgatac cattccatgc tcgacaagga tcacgagtag cgcatcaagc attttcgctt
    13981 cgttgggggt gggcagccgc ccccgtaaca tgagcgacgt catatcggta aaagtgattt
    14041 taccgaccag ctctttgtta agatcgtaac cgcgaacgag aatacgatga agctcggcct
    14101 tgccgatacc tgtcacgatt ttgggttgtt gttccgccat ggatcctcca attgatttag
    14161 atgggcggat tctaggccac gcacttacaa gccgcaacgg ctaacctgaa tttatctagg
    14221 agatgggata taagcgtctg gacgcacggg acgctcgcgt gtcagcccgc agcggttttg
    14281 cgtcaaccag tggaccagca ggaatacgct agtgatccct atccacgtag cgactccgct
    14341 aacgatcttc ggatccgtgg tcccgaacaa ccagcctagc tcatcgcgag tatagtcacc
    14401 aaaaactcgc accaacttca atccaaagac ccaacccgca tgcaagccga ttgatagata
    14461 taagtttcct gttcgaaaaa atgcataact taaaactact ccaagcaaaa acagaccgac
    14521 catgccggga aggaggttta acggttctga aaacggcgtg aaggtgtaga atagatgacg
    14581 aaatccggcc agtggatcaa agctatcgac gaagtaggct tctccgggcc tgatgaagtg
    14641 cagcgcagaa taaaataagc ttgacagcac aaaggctcga atgatcctgc cgttctgaag
    14701 cagccctttg aataaaatgc cgcgaaaaaa ggtttcttct aatgttccgg cgaacactcc
    14761 cgacaagagc gcgctaaagc aacgcgatag agatcgcgac aacgacagcc tgagataagg
    14821 ctcaaagacc tgagcgagcg acattgctaa aattaacgcc gcaaccgatg ccaccgccaa
    14881 gctccagccg cgaagcagat cgccggcgcc ttgaccaagg cctacaaacc ctagttcggt
    14941 aagattgcca attttaagca ggcgccgcca ggtgaaaaat agcaccattc ccgaaaccat
    15001 gaatgtgcga ttaaagatgc gtgagaaagg ataacgttcc acgccgagca cttggccggt
    15061 cacccagtca gcggcgatgg caaaccacgg gcttaaaata ctcgtcagcg ccagcacaaa
    15121 aatgagaaaa ataagcagtc tctggtgtgt gctcatagat ttcatggact agttaactat
    15181 ggtatacgat cggaagcatg acaagcacgc cccagaaaaa gttgatgaag aatgttgccc
    15241 tgatcaccgg agccactcgc ggcatcggca aagccgtggc cgccgcgtac gtccgtgaag
    15301 gcgcccgcgt gtttatatgc gctcgcgacc ggcaggaggt tgcggcaacc gtgcgcgagt
    15361 tgcgcgattg cagcacggaa gcacaggtcg atggttgcgc aggcgacgtc ggcaaagaga
    15421 ccgatgtttg ccggatcgtc gatgccgcca ttgaaagatt tgaaacgatc gacacgttgg
    15481 tcaacaacgc cagcttgctc gggccgcggg taactctcgt tcaataccca tttgagcaat
    15541 gggaagaggt gattcgcgtc aacttgaacg gcgtatttct tctaactcag caggtggtca
    15601 aacgtatgat cgcccgccgt cgggggtcca tcatcaatgt gtcctccgga gtcggacgta
    15661 ccggtcgagc gcgctggggc gcctatgcag tttcgaagtt tggcttggag ggactgaccc
    15721 aggttttggc tgacgaggtt aaggagtttg gcattcgggt gaacagcgtc aatccagggc
    15781 ctacgcggac cgagatgcgc gccgaggctt atcctgaaga agacccactg acattaccga
    15841 caccggaaca gattacgccg atcttcgttc atttggcttc tgtcgagtca gaagatgtaa
    15901 cgggtgcaat attggaggct aatgactggt tagagcacgc gaattgacgc gtttcgcgag
    15961 tagaaagctg atattttgtt tcctggagtt catcccggaa caatgacagc ctacgggctt
    16021 tcaacgcagc gagattattg gcgatgacat cactctttct ttatccaccc gacaaaatgg
    16081 ggtgggacgg aacttcgact gacatcgtac cgagcgttat taagagcgga atgggctggt
    16141 gaggaacctc tccgcacatc aggatcttca acggacgaag aaatgtctca agggatggaa
    16201 gataagaaat gaaagcgatt ccaatggtcg cgcttctcgt tatctatgcg ctaacgtctg
    16261 tcgcaaccga ggtccaggcg caagatctaa aaaagatcaa aatcggttat cccgcggtct
    16321 cttacaatca aattcatatc tgggtagcca aagaggcggg cttgtttcgc aagtacggcc
    16381 tggatgccga cgtcgttttt ttccgtggcg gcgggatggc aactcaggcg ctggtggcgg
    16441 gggatccgcc gatcgttaat atcggcacag tcgtgttggc cggactgcag ggttacgatg
    16501 tcgttctcat tggctcctcc gagagtacct ataactactc ggtcgtggcg cgtcccgtta
    16561 ccacgaaggt agagcaactc aaaggtaaac gattgggtgt aagtggcttc gggtcagcat
    16621 cgcatactgc cgcgttgatc ctgcttaaga agtttaatct tgaacccaac aaagacgtca
    16681 tgctcgtggt ggcggggcca actccggacc gcttggcggc gatggagtcg aatcgcatcg
    16741 acgccacgat tttgactcca tcggaaacac cgagggcgcg caagcagggc ttcattgaaa
    16801 tctttgacat gctcgatcta ggaatcgagg tgcaaggcaa cggatttgcc acctcccgga
    16861 cgtttattcg caatcagcgg gaaaccgctc tgagcgcgtt gaagggctac gttgaatcga
    16921 ttcactatat ccacaagaac cgggaggaaa ccaagaaaat cgtcgcgcaa tatctgcgaa
    16981 cggcggatgc cgaggctctc gaagcaactt ataccgcgtt cgtgaaaact gttgccaagc
    17041 ggccctaccc taccctcaaa ggaattcagt atttgctcga tgacttagct gtgaaaaatc
    17101 cgcaggcaaa aacggtaaaa cccgagcaat tcgtcgacct gagtttgctt caacagttgg
    17161 agaaagaggg atttttcgcc gagatggcaa agcgctatcc atagttgtta tgatccgcac
    17221 gcactgactc cctaaactaa ggaagaataa ggtatgaacc aacatatcgg caagcaattc
    17281 ccggatttcg agatgctcga tcatgacagc cagccggtta aactctcgca gtatgctggc
    17341 aagttcccct tgatcctgtc gttctatcgc ggctattggt gaggcaaatg ccaggtgcag
    17401 ttgcgccatt atgtggaatt ccaaaaagag ctggtgatca actattgtaa gctggtggtg
    17461 gtgagcgtcg atccgccgga agtcaattcg gcctttcgaa ctggcttggg cgccgagttc
    17521 ccgtttctga gcgatcaaga tcgcaaagtc gttcaagccc tggagatgac cgaaacctca
    17581 aagtcgcgcg gcgtgacggc aatcccttat accttttcgt tgatgccgga tttgacggtt
    17641 cacaactttt actgtggcta ctggtacgtc ggccgtccca ctctcgatga gctgcgtatg
    17701 gatttgcgtg cgatgatgaa gaaagtgcgg accgacttcg atccccaagc gtaaaggcaa
    17761 aaccgcgcga cttgcgcttg aaaggggcga atcaagtcgg taagattcgc ctatgatcac
    17821 tccggaacaa atcaaaagca ctctggaaca agcgcttccc gggtccacca tcgaaatgca
    17881 ggatctcacc ggcggcggcg accactggca agtattcatc gtctcgccgg cgtttgaagg
    17941 taaggggttg atcgagcaac atcagatggt ttatgcggcg cttaaagatg agatggggga
    18001 tcagcgtatc catgcgctgg cgctcaaaac ctttacgccg gagcaatggg ccaagcttgg
    18061 ctcatagtct ccagatagat aaggagaaaa ttatggcaga agatgtcctg aaggaaatcg
    18121 attctaaggt caaaaacaac aaggtaatgc tgtttatgaa gggtacgccg gattttcccc
    18181 agtgtggctt ttcggctcac acggtggaga ttttaagatc ctacggcgtc ccgttcgcta
    18241 cggcagatgt cctcgccgat gccgccgtgc gcgatggcat caagaggtat tcgaactggc
    18301 ccaccattcc gcaagtttat atcgacggca agtttgtcgg cggctgcgat atcgtgcacg
    18361 agctgcatga gcgcggcgag ctggaaccga tgctcaagtc ggcattagac aaatagacaa
    18421 tcaagctcat tgggagctag ggcggcaaat gggcaacgcg ttcgcgccaa taatcctcac
    18481 cgtggcagtg gcgctgttga gttcgtgcgc aacgctcagg agcttgaccc ctgggcgcga
    18541 cagtttcgaa gccgggatgg cttcgttcaa tcaaggccag ttcggttccg cgattgagca
    18601 cttcaagcag tcgacttcag agaatcctaa ctccgcgcaa gccttcctct atttgggtag
    18661 atcctacatc agcgcaggtc gctggaggtc ggccatccaa ccgctgcgca ccgcgttcag
    18721 gctgtcaccg catgaggcca aagacgagat catgaatctt atcgtcgatg cggttttcgc
    18781 ggcggcgaca aactatccgg gagaaagcgg ttcatccggt gaccgctaca aagatttgct
    18841 ttagcatccg gcatttccct ggattattct tttcagctga cttctcccgc gtccctctcc
    18901 aaaaaggagc gtctggtcat gttcaagttc atagcggcct gcttttttgt cctcgctatg
    18961 ctcactccgg cgtgggccca attcgacaga gtcttgaaag gattgggcgg gataaccggc
    19021 gcaggaaatg gcggccttaa tgacgccaag atcggatcag gcctgcagga ggcgcttaag
    19081 gtcggcactg aaaacgcagt gctccaaact ggttcgattg acggtttctt caaaaatcag
    19141 gcgatcaaaa tcctgatgcc aaggccgctt caaaatatcg agcagccgct gcgtctggtc
    19201 ggtcatgggc cgcaaattga cgagttcgtt ctcagcatga atcgcgctgc tgaaaaggcg
    19261 gtaccatatg ccaaggaaat cttctgggat gcaattggac agatgacctt cgatgatgca
    19321 agcaaaattc taaacggcaa cgataccgct gctaccgatt acttcaaagg caaaacttct
    19381 aacaagctgc acagcgcgtt caggcccacg gtaaaaaccg tgatggccga tgtcggtgta
    19441 acccgccaat acaacgactt gatcggtcgg tataaagcag ttccgtttgc tcaaagcatc
    19501 tcctttgata ttgaccagta tgttactgaa aaaacaaccg acggtctgtt cttcgtcgtc
    19561 ggtcaagaag agaaaaagat tcgcacgaat ccggcggcgc gagtaactga tttgctcagg
    19621 gacgtgttcg gcagcaaaag atagcttacc ggctggtcac tatggcttgc ggcgcctggt
    19681 catcgatcgg ctgccgcgca ttttcttacc tcgttctcac ggtgttcgtt gcggttcgct
    19741 gcactcagga gggcaagatc ctggacacgc gcacgcggac acatccatcc tcacgcgtaa
    19801 tcggaccgag tcgcggccga aggacgactc aaaattctga acgccgcgag gcttctaatt
    19861 tccgagctac tcaagactta cagaacagcc agagtcggga tttaattgat cgatgatgag
    19921 ttctggtcga gccttgcatc tctaatcgca atcacccaca atggcgccgt tttaagcgga
    19981 cgaggcaacg aaagttacgc tcttcggcac cgccttccgc ggttttaccg ctcatccagc
    20041 ttaagattgt tccgctcgga gtgaagccaa gctgcgcgaa cccgaggttt tatgaatagc
    20101 atctataaat attggaaagc attgaagcac gtcggagtgg tcacggttac tatcgtgatg
    20161 atagtcggtg ctattcattt tccctatgac attgacgcac cccttaccga gcaggagctc
    20221 gaggcagctc gaaaatacta taccgacgcc tatcgaaagc cggtcgaaga aaacaaagac
    20281 aaatccgaat acgagacgaa atatattcgg gttgcggagg ccgcggctaa aactgcgcgt
    20341 attgaagaac gagtatctga gttcgttagg cagtttaagc taagtgacag ggaggtcctg
    20401 gaaattggat cgggccgagg ctatttgcag gacatagcag aaaaatatac cgggttggac
    20461 atttctccga ctgtagcgcg cttctatcat aagaagtttg ttcttggttc ggctaccgca
    20521 atgccatttg cggacgacac ttttgacggg gtttggtcaa tttgggtgtt cgagcatgtt
    20581 cctaatccag agcaggcttt tcgtgaggcg cggcgtgtca cacgagataa cggagtactt
    20641 ttcctattac cggcttggaa ctgcacctct tgggcggccg agggttacga ggtcagacct
    20701 tattctgatt tcaacacagt tggcaaaatc atcaaagcaa gcataccact acgctcctcg
    20761 ccccctttta tagcaatgag caaattgccg aaccggcttg ttcggaatct cgtcgctcag
    20821 ttcggcccga cgaaattgcg ataccgccgg ctaagcccga actatgacca atactgggta
    20881 cccgacagcg atgcagtgaa ctccattgat cgttatgaag caatgttatg gtttcgtagc
    20941 agaggtgatg aatgcttgaa ctgtgacgac gcaccgctat ctctatctca catgcctctg
    21001 atcatccgtg ttcataaatc aggcacagag cttcagagcc gacctgatta agcgttctcg
    21061 cgacctttgg ttcgaatccc accggatcag aagcacacgg agaaaacctg gcccgatgct
    21121 ctagctgagg ttgtgtttac ctgcttgtag aatagctaga taagcggcaa actgaaaacg
    21181 actcgtctac aatcatcagt tttgatcatc agcgccctcg tctagcgcac cctgccgatg
    21241 gttatgcttg gcggagtatt ggatctaatc tgatgccaag aatcgcagtt cagttggcca
    21301 acgaagccat caagcgcccg aagcctgcga catcttcttt gacctttgcc attttcaagg
    21361 catgccagat gtatgcttgg caactggtta caactggctt gccgatctcg cgttccagta
    21421 aatcgacgtc cgtaatagtc ggccagcgcg ggcaaggaac aaagatgccg tctgcatcgc
    21481 ttgcctgttc atagagtttt ttggcgattc gataggcggc atgaatatcc atatctgtaa
    21541 ggtcggcatt cttacgcact ccatacccct gaattttcag caccttgaag cctgacgcct
    21601 cgagaaacgc cttcattctt tggttgagcg agtcttcatg cggcgttgcg accacgagct
    21661 tctttatatt gagcgatcga agcgcggcga tttcgccggt tatccccgga gaagttggca
    21721 caccgaatct ggcggttaac ttcttctcca tttcgatgtc gctgccgtaa ccgagcttgg
    21781 taaacagcgg tgacccgccg atgatgatcg catcacattt gttttcgacg agatcctggg
    21841 ccgcctcttc aatgcgttgc aactgtcgtt caaagtctgc atccaccaat tgacggatcg
    21901 ttcccgtgct gttcagtatc atgaagcctt tcggcatcat ttgatagaat tcgtacacca
    21961 gagtatcgcc acgcgatggg gcgacatggc cgatctttgc gcgccagccg tacatatttt
    22021 cctccctccc agaccgttga aaaagcaccc atctgcttcg ttccgctcgt tcaccccgtt
    22081 ccgacgtaca tcaagagtac gctttcgctc gttgaacttc acgcgcctcg cagctgttac
    22141 tttttgaccg gtctggaaac tccgtttaca gaattcgatt cctctgacaa actcgactta
    22201 ccagttgagc caggcgccgt tcacatgaat tgtctgaccc gtaatgtagc gattctcgtc
    22261 cagaacgaga aagagggcag tgctggcgac gtcgtctggg gttccggcac ggccggcagg
    22321 aatcctcgtc attgcttctg cccactgctc atctgaatag tggccacgca ctctaggggt
    22381 ggaaatccgc ccgggagcga tggcgttcac gctaatacct tccttggcga cctcgagagc
    22441 gagggacttg gtaaacaccg caacacctgc cttagcggcc gcatagcttg cacgggtggc
    22501 gtttccggtg aaaccataaa tagacgagat gctaacgatt ctgccatatt tttgtttcaa
    22561 catcaccggt aagaccgctt gacagcagag gaatacggat ttcaggttca tatcgatgat
    22621 gtggtcccaa tcggcttcga tcatctcgag caccgacttg cgatcggcgc cgccggcgac
    22681 gttgactaga atgtcgattc gcccccaacg ctcgagagtt gtctgaacca tctgctgcac
    22741 cgactttttg ttggtcacgt cggtgataat ggccaacgcg tcacaaccca tcgagttgat
    22801 ttcaacggca tggcttttcg ccgtgtcggc aacgatgtcg gcgagagcga gtttggcgcc
    22861 ttctcgcgca tagcgcagcg cgatggcacc gccaattccg ccgccgccac cggtaatgat
    22921 cgctactttg ttttctatgc ctgccatcga gtataaagcc cttcatcgaa gatgatgaag
    22981 tctgcgaaac cagaagacct gacgaatcta ggcaaattgt cccggtccgt caatatcaat
    23041 cgttgtcagc aatgatgcca ttgtgttaca gacgttctgt ttatcagtga ctacaccgag
    23101 ggagaggaga aatgaatgca acaagtgctt gagcaggttg atcgccagcg aattgttgaa
    23161 atcgcctgca aactggctga tacggtgagc atcaccggcg aggaaaagga agtcgcagaa
    23221 tacctaggca gcgaatttga gagactcggg atgcaagtcg aatatcaaga agtcgaagag
    23281 ggaaggccca acgtcatcgg cacgctcaag ggcagcggcg gcggcgcgac tttgatgttt
    23341 tgcgctcaca tggaccattt cgacaacccc caggaaaccg tggtcgaaga tgaccgtatt
    23401 tacggccgcg gattggtcaa tatgaaggcg gcgtttccgt gctatatcga agcgatagcg
    23461 gcgctgcaaa aagcaggcgt tcaattgaaa ggtgacctga taatctccgg agttgttggt
    23521 gagatcgaga aagcccagat cggcagattt cagggtaaga attatcgcgg cgccggcgtc
    23581 ggcgcgcgtt atatgatgga tcatggcgtg atggctgata tgtgcatcat cggcgagcct
    23641 accggcctgc aattgcaaat cggcaacgcc gggctgttat gggcgcggat cagcgtcgat
    23701 gggcgcgcta ccaaattcgt tctcgcagcc tgcaaggtgg cgcaggcgat tcaggattgg
    23761 gaaaaggaat atcaaaagaa aaacccgcac aagtttatgt tgccgacggt gcaaatcggc
    23821 gccattgacg ggggtaacgc gttcaagcct ggaacacggc cgacgaccga tattttcgtc
    23881 atcgtcaaga tgctcccggg cgccgtgccg ctggcgatca agcgtgatat tgaacagctg
    23941 tgcgagagag tgcgcaaaag agaaccggat attctggata ttcgagtgga tctctattta
    24001 accacccccg gctatgagat ttccaagcgc gagtacgtcg tcaaggcgat ggaacaggcg
    24061 caccgaacgg tcttcaacca gccggtaccg tacgccaagc ctattcgtta tggtattacc
    24121 agcgatggct cgcgcatcgc cgcctatggc gtgcccgcga ttacctatgg tccggggttt
    24181 gggactcatc tggtcgatcc gaccgagacc aaagccggtc cggaatggga tgcgcccagc
    24241 ggagttcgcc gcggcgtcgg tatcgaaaac atgattaatt gcaccaaggt gtatgccatg
    24301 gccgcgctcg atatcgtcaa tcaaaagaag gatgatgtcg ccaaaacgtg aagggagccc
    24361 gatgcagatc acattcgccc attatcgtaa tcgtttctgc atgtatcgaa cctactacgc
    24421 gctggccaag gggatcgtca agcccgatgg ctttacgatg aaagtcatcg aattgccgga
    24481 cccgccgagt catgaacagg aggacgcgtt gattcgcggc gaggtgcaag ttgccaatct
    24541 ctacctgcct aacttcttgc gacgcaaatt agcgggggcg ccgatcgtag gcctatgcac
    24601 ggaatggaag tcgacgatga aaggaaacgg catgttcgtg cgcaaagacg gcccggtgca
    24661 cgatccgcgc gatctagcgg gacgtgtcat cggctcgcat caggggcccc atgccataca
    24721 ccgatatttg ctacgccaca aattcggcat cgatgatgcc ggtttgcagt gggtctccta
    24781 tccgcaggag caactgctgg aggcgatgaa gcggggagat gccgacgcag tggtgctgct
    24841 cgatcagttc ttcttccatg gcgaacaaga tccggaagtc cagtgcctgt acaccgatgg
    24901 cgagatttgg cactcactgt tgggcttttc ggaaatcatc aagcacatga tcgctatgga
    24961 agaaacgttg tgccgcgagc agcccgagtt gaaggaaaag ctgcaagccg cttttcgcgc
    25021 gtctttcgcc tatagcgagg aacatctgga ggaaattgcc ggcgagttcg ttaaaaaata
    25081 cccgggcgat aagcaggcgt tacttgcctc cgctcggtat ccgaaaatcg aattcacatt
    25141 gaccgaaacg gagcaaaagc tggccgacgc ggagatggac atgatggttg aagtcggttg
    25201 gattccgcgc aaggcgccgg tgcagtctct gtttgcgatt tagatgcaat actttttggc
    25261 tcccctcggt atggacgtag attttgcttc ggcccagaaa ttttctttat agcttcgagc
    25321 gagcagtcgc atccatcatt tttatttttc tcgcctcttc ctgtaccttg atgggggtgc
    25381 ccatccggaa cgaaacagag ttgcttcgcg atgcttcggc cacgatccag gagatcaaag
    25441 cattcgaaaa agatctggga atcgagccta ctcaagcgct ggcccaaact agcgagcaaa
    25501 aaactccaac ttcgatgttg tggttctggt tacaacgcgc cggtaccctg gctttgcgaa
    25561 ccccgatcga tgtccgtttg accttggggt tcgccaagtc aaaggaagag gttgcgctcg
    25621 atcaggttta ccgagtcgac ggttacagcg tttactatcg ccagggaaac gagttcgcag
    25681 atccccgatc ggtgacgacg aaaagctttg cgcgcgcggg tgccgtgcgc caggttgcgg
    25741 tggtgatcca tgaggatttg cacggtgagg ctaattttga cctgccatgg gagatcgaag
    25801 agtctctaat tacccccctc gggtcggtgg ccgcagtgga gttcttcaag cgcaagtcgg
    25861 attcagccaa tcttgagagc gcccaggaac gactggacga ggaaagccag ctatcacgag
    25921 aactcggcca attggtcacc gtggcggaaa caatctttag caatcaatct gtcgatagcg
    25981 ccaaggagaa aatcgtggcg atgattcccg cttatcccgt ttatcatcgc tatttccagc
    26041 gccaaatcca agggcagcat ccggagacgg cgctcgaagc taagctcagt cacgatttgg
    26101 tctactatcg ttatttcgat caaatcgtag ccctttacga gaccaccggg gacttaaagg
    26161 cgctggtgcg agatctgcgc aaggcgccgc gagacgccca agcactggcc gcttatcttc
    26221 gatccttgga aaaaagctat gcaattgcga actgcgattg attctaaagc gtgagcaatc
    26281 acgaaccacg cgcattccga atttctaact tcgcgagagc gcggatgaag tgcgagtgcg
    26341 ccaacaatga tcaggtgcaa atcaagcccg ctctataacc gtcgcttcga actcccgctt
    26401 caagtgttat catcttgata tcggtagaag gccgtgctgg gctttctatg acgattacgt
    26461 ttatctggag gcctatcgag gagaccgatg cgcattttta atttctttgt gctggcggca
    26521 atgctgtgct ttggcggctg cgcgagcacg ccaatgctcg taagccaatg gagcaatccc
    26581 gcttacgaat cgccttactt caaaaaaatc gcggtcagtt gtgtcgatgg gcacatcagc
    26641 atccggcgca actgtgaaga tgagtttgtc gcgcggctca gagccgcggg cgtcaatgcg
    26701 ctgccaagct atcgaaattt ttccgaagat gcaaaacttg atggagcgaa actgaaacag
    26761 atggccaaag aagccggcgc cgatgctgcc ctgattgttc gctccgtcgg agttgaagag
    26821 aagacagagg tcagtcctac ttactttccg gcacccgggt tcgggatttt cggccgaaat
    26881 atttctgcca catggtacgg ttggtacggc gcgccgcgag tttaccgata tgaggagtat
    26941 acgtcggaag cgactctgaa tgatctaatc aagaatgaag tggtctggac aggtaccctc
    27001 aagaccaccg catccgacaa tgtcgattcg gcgattaaga cttatgtgga caacgttatc
    27061 aaggcgttaa acgataggaa cttgcttggg gccaagcagt aaagcttttg ctgttgtgac
    27121 gaaccacctg acgcgtatga ccgtggaacg tgttgcgata ggttgcgtaa ggctatgtgt
    27181 ccttggacga ggcttgccgg ggacgcctga cgtccgaaca ggacatataa cctttgagcg
    27241 gcgcaactgt ggaaggggcg cagagtctca cgcgcgttga acagtgatcg gttcaacgtt
    27301 ataagcgggt gtacctgcgg taacggatct tacaacgcga gcaatgagta aacggcgccc
    27361 agtcgaagag gaagtcatgc caaagaagac cgctaagttt cattcaaaca ggagcggcgc
    27421 aagcagtgga aaggaaactc tatgaaaaag atattagttg gatacgacgg atcggagcat
    27481 gccgacgccg ctatcgacga tctgcggcgg gccggactac tgcaggatgt cgaggcgatc
    27541 gttctgaccg tggcagacgc attcgtgccg cccgccgtag acgcaaagga ttggggacct
    27601 tcgccgctgg atgagaagat caggaagaga agcgaagaaa tgcgagcgcg agctttgaaa
    27661 gcccgggacg aggctgcttc tgtagcggct aaaggcgccg cccgcattaa ggagttgttt
    27721 ccatcatggg cggtacgtgc cgaaacagat gtaaattggc ccggctgggg aatcattttg
    27781 aaggctgaag aatggcacgc cgacatggtt gtcgtcggcg ctggaaagca ttcgtttatc
    27841 gagcgattac agatgggcag tatactgcga aaagtcgtcg gcgcctgtga acgttctgtc
    27901 agagtggggc ggccttcttc cagggctcag gagacctcgg tgcggattat catcggtcta
    27961 gacggatcgc ccgacgccga gttggcaatg gaggcggtgg cggagcgcgt atggcccgcc
    28021 ggaagcgaag cccatttgat gacggtgctc gataacaggc tctcgattct tacgccgtcg
    28081 ctcatccctc gtttggcgcg ttggtcgagc gcggcagatt cggttgatga tcaagcatgg
    28141 gtggagcgga tgatgaaagc cgccaccgat aagctacaaa ctgcggggtt gaaaatttcc
    28201 tgcgaagtcg taaaaggcgc tccgagccaa cgccttcttg atgccgcggt agattggcaa
    28261 gcagactgca tcgtcatggg agcgcggggg ctaaccggga ttgaacgctt tcttatcggc
    28321 agcgtttcgt ccgatgtcgc tgtgcgcgcg aactgctcgg tagaagtcgt gcgctcgacg
    28381 cgatcgcgtc catgagccgc aatgatgcaa tcctgtgaaa cggcgattac tggttggcgg
    28441 tccatttctc catgcgagtc ggcacgagat actcaaaaat taaacgtctc actcgcgcgc
    28501 ttttaatgcc gcgcttgcgc cgatctcttg tttatcgcgc tcgggcgaga taacatgttc
    28561 gccatggcca gaattactgt cgaagactgt ctttcggaaa ttcccagccg tttcgaactt
    28621 gtcatactcg cggcaaaaag agcaaaacag ctcctcaagg gagcgaaacc tcttttggcc
    28681 tcggataacc gagctatcgt cgtagctctt cgcgagatcg cggcaggcaa ggtccgccgc
    28741 gcggcgccgg aacctgaagc gttaccagca cccgagcgga cataagcccg ccttcgcctg
    28801 tcctaatctc ttgttgcgag ccaattttgt tgccggaaga atctacgttg gtcgcagggc
    28861 agcgtagctt gcaggcagca tcgccgaaca tcgatgcgat ggcgccgaca atccacttat
    28921 tgccaaagta gattatttaa cgcgctggtg cggcgcgtcc ttgtccgaat cgacgtcgaa
    28981 caccgagatt tcgatagcca gcttggctca tctgaatgcg tcttatcgcc cacccgccgg
    29041 agaccgtgca gttccaccgt ggatgtgagt attctctagg ctacttgtgc tcagcatatt
    29101 tggacgtgag taggtgctaa tatgtatcga acatcctctg aatctcgtcg gcattgttgg
    29161 ttttagtaag cgccaaagag agcagcaggc gcgctttcca tggttgcagg ttatcgcccg
    29221 cgacgaaccc ccgcttggcc agaccggggc ttcgcactac ccggccggag gcgacccggc
    29281 tgcagaggca cataagcatt cctttctctt tgaaagcttt gtcgaaagcc gcgtcttccg
    29341 ccggtgtcgg ccgtccagcg ccggtgcctg cactgacgat gcctttggct cccgcgtcgg
    29401 ccgcggcttc gatcatagtg ccatcggctc ctacatagga gagcactatg tcaacgcgcg
    29461 gcaaggtttg caggttgcgc acatcgaact cagtgtcaac ggtgtgtttt cttacaggtt
    29521 ggtgatagta aacgatcttg ccgtcgccgt cggcaaatcc cagcggtccc aaatcgcgcg
    29581 actggaaagc ttcgacgcgg taggttgcgt ttttcgtgac atcgcgtcca ttgaaaatcg
    29641 tatcattcat caccagcatg cagccacggc cgcgcgaaga tggttcggcg gagactttga
    29701 tggcgttgac cagattcaaa tacccatcgg cgctaatcgc cgaggccggg cgcatcgagc
    29761 cgacgaccac caccggctta tctgttttta gagtcagatt cagaaagtag gcggtctctt
    29821 caatggtgtt ggtgccgtga gtgatgacaa tgccatcggc cttattatcg tcgaaaatcg
    29881 aatgaatagt tcgcaccaga tcgagccagt ctttatcgac tagagcatga ctcggtaaac
    29941 ggcgaaatgg aatttcttgc acctgggcga tatcttttag ctccggaagt tgctgcagaa
    30001 gctctccgtc accaagtctt tttcctgcct cgatatacca ggcaagatct aaacggtcct
    30061 tgcccagcga gtcgatggtt cctccggtca ggatcaatgc gatgcgcggt aaattggcca
    30121 tgttaactga ttcctttctg tgtcactggg aaatagtgat cttctgcgaa gcgtattgaa
    30181 ataattagct taaggacggg agaatttcta ctatctgcaa tccggtcgct attcttctgt
    30241 aatcgctgcc aaccattcgt gcagctctag ctacgggccg catacctgta tcgaggaatt
    30301 tctggtgttc gtcggacgat cagctgcgca aagccggcaa ggcgtctctc gcaaagtgct
    30361 cgatctgttg ctgacgttcc ggataatgcg gaatcatgaa ctgcaggcca atgtgcgtga
    30421 ctccaacctt ttggaacatt ttcaatcgat cgctgatttg ttcaatactg cccttcaagt
    30481 agtcttcttc ttgatgcgaa tctttggctg tcaattcgat gggcaaacag caggccagga
    30541 cgagttcatc cggccggcgc ccggattctc tggcgatttt tcggacgtat tggaagcgcg
    30601 ctgctagttc cagcggggtg attcgcacga agtaaggaaa ccaggcatcg ccgtaacgac
    30661 cggcccggcg ctgagcgtac ttgccttcgc cgccgaccca gatcggaacg cgcggctttt
    30721 gaaaaggctt ggggttgaaa gcgatatcgt cgacgttgta gtacttgccc ttaaaggtga
    30781 tgtgctcttc ggtccagagt tgcttgagca aagcgagctg ctcgtcggaa actgtgccac
    30841 gctctttgaa cggcgcgttc atggcggcaa actcttcttc catccagcct acgcctaccc
    30901 ccatgatcag tcttccagtt gagagttgat cgatggttgt cgcaatcttc gcccaatgaa
    30961 gcgggtgacg atagggcaga accatcacgc tcatacctaa ctgaattttt tccgtgcatc
    31021 cggccagaaa agcgagacaa gtcatggtgt cgaagaacgg ccgatcgggc ggtacgatga
    31081 acgtcgactc cttgctataa ggataagcag tctcgatttt ccatgggatg acgagacgat
    31141 ctgcggccca tactgaatcg taacccaggg attctgcctg ctgtgcggct tgcatcaggg
    31201 tcttacgact ggcagcgcgc ccggaaaccg gtaagaagac gccgtacttc attccgtacc
    31261 ctccacgaac attctagatt ctgctgaagt tagctttgtt cggcacagca atcatctagc
    31321 acaaagcagg ctcgatcgcg atttggaatg aattctaatg agccggactc ttggtagctc
    31381 catcttcggc gggttacgag actgatacgt aaaaggcaag cagcggtgtc gcagcgcatt
    31441 ttcaatgtgt caatgcgatt gcaactacgc cggcaacgac cagcagtgtg ccaacagcac
    31501 tggcgatggt gatcctttca acctggcgga gaaagattgc gctaatgatt agcgtccaga
    31561 tcggagtggt cgaggcgatc ggcgagacaa ccacgaccgg tcccagcgag aatgccagga
    31621 gcatgaggag aaccgccagc gtctcaaaaa atccggagac gaaaaacggc cagaacgctc
    31681 gtcgatccca gaccagtttc tctttgcaga taggcgtcag ataataggct gcgagcgcga
    31741 tgagagagac cggaccgacc aacgccgtga aaaacaacgg ctcatgcgat tggatcaatg
    31801 catagcgccg taacgggtgg actaccgcgg taatcagggc ggttgcgatg gggtacatga
    31861 gatgcgtcca atgaaaagat ggcaaatgct tgtcaatgcg ccaggaagtg agaacgatgc
    31921 ccgcgactac cgaaagcgtt ccggcgatgc cgagaggggt gattggttcg ccaaggaaca
    31981 cgacgccgat gagcactgcc ggcagtggga aagtattacg cagcgtaacg gcccgggatg
    32041 tgccgagctt gtggatgccg gtgtaatggc agtgccgcat gatcggctgg agaatgccgg
    32101 ttacgacaat cgcgacgacg gctgctaatg caacccgggg tatccccata gtcagaaaga
    32161 ccacaatcca gagagccacc gtatggatga tcaaggaaac taaagtggcc gtgaaaggag
    32221 tcgagtagtt cagcccgtca cgcaccgaaa cattggagag cgcgaatgaa acggcggtga
    32281 gccaagcaag aagttcggga gacataggat tgctggtaag tatgcaggca gacgtctggg
    32341 gacgtgccga ttcgtcggaa gtcgagagct tggaaacgat ttgaagcgcg tcaaagagtg
    32401 ctcggttgca agcttaggga cagcgtaggt tcacactccc tcgtagcgat cataactttc
    32461 cgaccttaca ggactcgggc tcttcctgga agagctgtac agaatcctaa cgcaactctg
    32521 gtgtcttgac gatgttttgc tgtctctgat ccttgaatat ttcactggct tgcaatagtg
    32581 cattcgacaa gagtagaagg agagtcaaca ttttgcccgg ttacggag
//
LOCUS       DASUVS010000464         8113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1737947, whole genome shotgun sequence.
ACCESSION   DASUVS010000464 DASUVS010000000
VERSION     DASUVS010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737947"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /locus_tag="VF182_24435"
     CDS             <1..1441
                     /locus_tag="VF182_24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEX6440288.1"
                     /translation="RSRASEFLALALIMILAPGAEWHRLAYAADTDGAVVATSLLDQE
                     AVSITVYNSNLGLVKDARRLRLPRGVIQLKFGEVAAKIMPQTVHSKSLSDPKRLQILE
                     QNYEYDLLTPQKLLEKFVGKEITILKDGVEVPITILSVNQGIVYKLGGRIFSGQPHNL
                     IFPSIPDTLISHPTLLWLLENHSTAPHQLEATYLTGGLSWRADYVAVLDSKDKLLDLS
                     GWVTLENQSGATYQNARLKLVAGDVNRVIERRGAADAMRELSELSAKPAAAPFNEESF
                     FEYHLYSLQRATTIKENQTKQVSLLSADRVPITKQYVYRGSQQYFRSRLGSPVSNQKI
                     GVYVEIANKKEQNLGMPLPKGVVRVYKADNDGSLQFIGEDRIDHTPKDETIKIKMGDA
                     FDIVGQRKQTDWRKLADNLYEAAFEISLRNHKEDAVTVSVIEPMLADWEILSSSHTHK
                     KTDAHTAHFDLPVAKNGETKLQYRVRYKF"
     gene            complement(1799..2470)
                     /gene="tenA"
                     /locus_tag="VF182_24440"
     CDS             complement(1799..2470)
                     /gene="tenA"
                     /locus_tag="VF182_24440"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEX6440289.1"
                     /translation="MAEKFSAVSRHRSGRIWRAIENHSFLRELHAGTLPMDRFTYFIL
                     QDYVYLLDFAQVLCQGAAKSPDLATLEMFARHALGAVEVERSFHASFGKTLGLSREQL
                     AAVKKGPITQAYIGHLQAVARSGSLSEIVAAVLPCYWIYGEAGRRLRKGAPSKPKVYR
                     EWIETYASEDFWKPVREQIQLMDILGAAATPREKKLLVDHFVLSSRYEFMFWEQAYRL
                     EQWPI"
     gene            complement(2602..3516)
                     /locus_tag="VF182_24445"
     CDS             complement(2602..3516)
                     /locus_tag="VF182_24445"
                     /inference="COORDINATES: protein motif:HMM:NF006015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX6440290.1"
                     /translation="MAKRSAGEANATDPSRLYLAKLGNEVRKLRQQRGITLKRLAQLS
                     GLSDRFLIEVEKGKANPSLKSVINLADALQTTLTNLLPGDETKTQNSSTSVKQVLTLL
                     KNRPDEQIARILSCLSSYLDDSEGPHISLIGMRGAGKTTVGSILARNLKVPFYELDAL
                     IVKDTGLSLREIFDLEGENYYRAVEERILERVLKKTSGVIAAGGGLVMNPNSLLRLKL
                     HTFVVWLQAAPETLIERAQTGKDKRRLSAHPDVRKQLKAILDRRTPHYAQADLVVNTT
                     DKSAEAISRTILNAFRSPANSKATQLSS"
     gene            complement(3516..4232)
                     /locus_tag="VF182_24450"
     CDS             complement(3516..4232)
                     /locus_tag="VF182_24450"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEX6440291.1"
                     /translation="MFNDKVTEKDVVKMDPEFQDLLKRVLAIQADCEIGGPHLYVKDI
                     LPNAPTKADQIMVAKTAAEEMDHFRKIARLAGDIGHDVSYVLSWPNQKRYVEAFRGKI
                     NTWEDFAIFGYLIDRVGRYQLEEFVGCSYQPLSRILPQIIKEELGHVGYGGSKAAELA
                     AKGDESRERLQKAVDFWYPKALDMFGRSDSKRSERYRYWGLKRRSNEQARNEYIKEVN
                     PLIVKLGLQIPGVEKNRLHQ"
     gene            4557..5639
                     /locus_tag="VF182_24455"
     CDS             4557..5639
                     /locus_tag="VF182_24455"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017654472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX6440292.1"
                     /translation="MKREISSYLQSQAQSNGPIRQAGINSNRWYMIAKSSDIQGQPAK
                     REIWRQAVVLFRTQQGEVRALEDRCAHRLVKLSYGRVKDDKIECAYHGWQFDGAGRCV
                     HIPHLGSRKTLPNCEVRSFPVVEKHGFVWIFPGDPALSDTVAPMEMNEWDDLNEIASF
                     VPLRTRAHFSYLIENLMDMYHGRLHAQYQVWTAESLKEVVEDENHVSATYQATTYYQV
                     KDLGSILQLFIPSLRKLHSVPLTVTYEYPNWKSALGEDFKIFCLICPVNERLTDAYLI
                     HYTSLAKFKGLNHAPLAVRRLLKRALSNVAKKLLANLVRQDVVMIEDEQAAFDQDPLR
                     QPFEVNRAIRRVQGLVRRQATEESLH"
     gene            5676..7190
                     /locus_tag="VF182_24460"
     CDS             5676..7190
                     /locus_tag="VF182_24460"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6440293.1"
                     /translation="MIKSFLRSKIKNKLSGYSIVINQPDPTKPKFVQSAKKVAVVGGG
                     LAGIGAASLLAERGFEVTLFERNHYLGGKVGCWDVSFENGNMTKVDHGFHAFFRHYYN
                     LRSFMEKIGAADHLKTIADYLVLAKDGRHFSFKNVEATPVLNILSLGRNKFFKFRDVL
                     FNPPSWRMGEFLEYDQTKTFAKYDSSSFAEFAKEASLPESLQLIFNTFARAFFAPADK
                     LSTAELMKSFHFFYLSHNHGLLYDYFDTDYQEALIQPATQHLNQHRVSIKLNKSVEET
                     GRDNDRFIVESEPFDYLILASDVVGTKSICANSPWIRTSSEKTYSELMSLKASGGYAV
                     YRIWLNKRTGEELPVFIITEKHEVLDSVTFYHQFDRSAAEWAQGSGGGVYELHCYALP
                     FRELSERDVKAAFLAELHFYFPELQDATIIYDHLQLRRDFTAFYTNLNKSRPEFTTAI
                     PNLYLAGDWVKTGIPAMLMEGAYTSGVLCANGILAKHGLQEEPVRSVPTRGIFA"
     gene            7216..7908
                     /locus_tag="VF182_24465"
     CDS             7216..7908
                     /locus_tag="VF182_24465"
                     /inference="COORDINATES: protein motif:HMM:NF039186.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HEX6440294.1"
                     /translation="MSLTYLVLNLCIIAVPVWYTPDKRTAYYRRLPALGFSIAVVSTS
                     YLLWDILVTARGEWSFNGKYLTGVQILNIPLEEILFFITVPYSCLFIYEVVLYATKAS
                     KFRLPASAIIALILALFAASLGVYSQGYTAKALASCGVFLTSALLLDRPLIESKQYWT
                     WLAICYVPFLIINTVLTALPVVEYNPKAIWGLRVGTIPLEDFFYNFAMLSFYLLFYRL
                     AKSWTARTVPLV"
BASE COUNT         1926 a         2212 c         2023 g         1952 t
ORIGIN      
        1 ccgcagccgc gcatcagagt tcctagcgct tgctttgatt atgattcttg ctcccggagc
       61 ggagtggcac aggcttgcct acgcagccga tactgacggc gcggtggtcg caaccagcct
      121 gcttgaccaa gaagcagttt cgattacggt ctacaacagc aatctcggct tggtcaaaga
      181 tgctcgccgg ctgcggttgc cgagaggcgt catccagcta aagtttggcg aagtagcggc
      241 taaaatcatg ccgcaaactg tgcatagcaa gtcgctgagc gatcccaagc gtttgcagat
      301 tctcgaacag aactacgagt acgatctgct gacgccgcaa aagctgctcg agaaattcgt
      361 cggcaaagag atcacaattc tcaaagacgg cgtcgaggtt ccgatcacga ttcttagcgt
      421 gaatcaggga atcgtttata aattgggcgg tcgaattttt tcgggccagc cgcacaatct
      481 gatctttccc agcattcccg atactctcat ttcccatccg actttgcttt ggctgttaga
      541 aaaccacagc accgcgccgc atcaactcga ggcaacttat cttaccggcg ggctcagttg
      601 gcgcgccgat tacgtcgccg ttctcgacag taaagataaa ctcctggact tgtccggttg
      661 ggtgaccctg gaaaatcaaa gcggcgcaac ctatcaaaac gcacgcctca agctcgtggc
      721 tggcgacgtc aaccgggtca tcgaacggcg cggagccgca gacgccatgc gcgaattatc
      781 ggaactgtcg gcaaagccgg cggcggctcc ttttaatgaa gaatcattct tcgaatacca
      841 cctttacagc ctgcagagag ccacgacgat caaggagaac caaacaaaac aggtgagcct
      901 attatcagcg gatcgcgtac ccatcaccaa gcagtatgtc tatcgtggct cgcagcaata
      961 cttccggagc aggctcgggt cgccggtcag caatcagaaa atcggcgtgt acgtggagat
     1021 cgccaacaag aaggagcaaa atctcggcat gccgctgccg aagggggtcg tgcgagttta
     1081 caaggctgat aacgacggca gcttgcaatt catcggcgaa gaccgcatcg atcacacacc
     1141 gaaagacgaa acgatcaaga tcaagatggg cgacgccttc gacatcgtcg gccagcgcaa
     1201 gcagaccgac tggcgcaaac tcgccgacaa cctttacgaa gccgcattcg agatcagcct
     1261 gcgcaatcac aaggaggacg ccgtcaccgt gagcgtcatc gagccgatgc tggcagattg
     1321 ggagattttg agcagtagtc atactcacaa gaaaaccgat gctcacacgg cgcacttcga
     1381 tttgccggtg gcgaaaaatg gtgagacaaa actgcagtat cgagtccgat ataagtttta
     1441 gcagcctgct aaaagtgctc catatacttc ctgatatttc gtccgatcgc tcaccgtctc
     1501 acaatcccaa aagctccaca tctgctccga caatccccct ccatagccat gtcgcgagct
     1561 tctgacacac cggcccgctg gtgcgtcaac cgacagcgat gatcaacgaa ctgcgcgcgg
     1621 tttacgccaa gagaaatatc gatccgcaag ccaagctgca gcgggtatag cccagtgtct
     1681 cgggggcgac gatctcagcg gctgtcgaca aactgtttca tgaaaggcct cctctaagat
     1741 tcgtgctacc tgtttatcac agcgcgtcgc gcgggggccc attccgttta tcagtctatt
     1801 agatcggcca ctgctctaat cgatatgcct gctcccaaaa cataaactca tatcggctgc
     1861 tcaggacgaa atgatccacg agaagttttt tctctctagg cgttgccgca gcccccagta
     1921 tgtccatcag ttggatttgc tcccgcacag gcttccaaaa atcttcagag gcataagttt
     1981 cgatccattc ccgatagacc ttcggcttgc ttggcgctcc cttgcgtaat cgccggccgg
     2041 cttccccata gatccagtag cacggaagaa cagccgcgac gatctcgctg agactgccgc
     2101 tacgcgccac cgcctgtaaa tgaccgatat acgcctgtgt gatcggcccc ttcttgaccg
     2161 cggccagctg ttcacgcgat aaccccagcg tcttgccgaa actcgcgtga aaactgcgct
     2221 caacctcgac cgctcccaat gcatgacggg caaacatttc tagagttgca aggtcgggcg
     2281 acttggccgc gccttgacaa agtacttgag cgaagtcgag caggtagacg taatcctgca
     2341 gaatgaaata ggtaaaccga tccatcggca aggttccggc gtgcaattca cgcaaaaaag
     2401 aatgattttc gatggcccgc cagattcgcc cactgcggtg tcttgatacc gcgctgaatt
     2461 tttccgccat tggccagggt tagcggaaaa ctgcttgcaa ggaaagaggg aggcttggct
     2521 caagcgatgt tatcgcccgc gcattcgttg gatttcatga cccgtggcag ataaacacga
     2581 cagcgtattg tcccgacgaa ttcaactcga cagttgagtt gctttggaat ttgccgggct
     2641 tctaaatgcg ttaaggatgg tcctgctgat tgcttctgcg gacttatcgg tagtattcac
     2701 aaccagatcg gcttgtgcgt agtgaggcgt tctgcgatcc agaattgcct tcagctgctt
     2761 ccgaacatca ggatgcgcgc tcagtcgcct tttgtccttt ccggtctgag cgcgttcgat
     2821 caacgtctcg ggcgcggcct ggagccagac cacaaaagta tgcagcttca ggcgaagcag
     2881 gctgttggga ttcatcacca gcccgccgcc ggctgcaatc accccggagg ttttcttgag
     2941 taccctctcg agaatccttt cttccaccgc acggtaatag ttctctccct ccaaatcgaa
     3001 aatttcccgg agcgaaagtc ctgtatcttt cacgatcaaa gcatcaagtt cgtagaacgg
     3061 cactttcagg ttccgcgcca aaatactgcc gacggtagtt tttccggcgc cgcgcattcc
     3121 gatgagggag atgtgaggac cctccgagtc gtctaaatag gatgagagac agctcaagat
     3181 tcgagcgatt tgctcgtccg gccggttttt caaaagagtc aaaacctgct ttacagacgt
     3241 gcttgagttc tgcgttttag tttcatcacc gggcaaaaga ttcgttaatg tagtctgcag
     3301 ggcatcggct agattgatga cgctctttaa cgaaggattg gctttgcctt tctctacctc
     3361 tataaggaag cgatcgctga gcccggagag ctgcgccaga cgcttgagag ttatgcctcg
     3421 ttgttggcgc aacttccgga cctcattgcc cagctttgcc aaataaaggc ggctaggatc
     3481 agttgcattt gcttcgccag cgctccgttt tgccattatt gatgcaatct gtttttctcc
     3541 acaccaggta tctggagtcc gagttttaca atgagtgggt tcacttcttt gatgtactcg
     3601 ttgcgcgcct gctcgttgct gcggcgcttg agcccccaat agcggtaccg ttccgaacgc
     3661 ttggagtcgg agcgtccgaa catgtcgagc gcttttggat accaaaaatc gaccgccttt
     3721 tgcagacgtt ccctgctctc atcccctttg gccgccagtt cggccgcctt acttcctcca
     3781 taacccacat ggcctagctc ctcttttatg atttgaggca gaatccgtga cagcggttga
     3841 taactgcagc cgacgaattc ctcaagctgg taacggccga cccgatcgat cagatagccg
     3901 aatatggcga aatcttccca ggtgttgatc ttcccccgaa acgcttctac atagcgtttc
     3961 tgattcggcc agctgagcac ataggaaaca tcgtgtccga tatcacccgc taatcgggca
     4021 atcttccgga agtgatccat ttcttccgca gcagttttag ctaccatgat ttggtccgcc
     4081 ttggtcggcg cgttcggcaa aatatctttg acatacaggt gcggcccgcc gatctcgcaa
     4141 tcggcctgga tcgctagaac tcgcttgagc aaatcctgaa actcagggtc catttttacg
     4201 acgtccttct cggtcacctt atcgttgaac atcttcacct ccattcactg acaatttaac
     4261 tgtaccaaag acgcacttta atgcaaccaa tttgcaaata ctgaagtgaa atataattct
     4321 gcttttatgt tgggcgtcat ctatggagct tcaggaaaag aaccgtgcca aaagtcgcga
     4381 gacttgtgct acgagggaac taggtcgggc tttcggaatc ccgctcgctg ctcgcctctg
     4441 catgcctgag tgtttttctg cgagcgccgg aaggtgaaac acattttcgc tacgttcgct
     4501 tacttgtgca ccatagcaat tctgtttatg tttcatgttt cagaggcggc taacacgtga
     4561 agcgcgaaat cagtagctac cttcaatcac aggctcaatc taacggccca atccggcaag
     4621 ccggcatcaa ttccaatcgc tggtacatga tcgccaagtc gtcggacatt caaggacagc
     4681 ccgccaagcg ggagatctgg agacaagcgg tcgtgctctt cagaactcaa cagggcgagg
     4741 tgcgcgcatt ggaagatcgc tgcgcgcacc ggcttgtgaa attaagctac ggccgggtga
     4801 aagacgacaa gatagagtgt gcttatcatg gctggcagtt cgatggcgcc ggtcgctgcg
     4861 tgcatattcc acacctgggc tccagaaaga cgttgccaaa ctgtgaagtt cgatcgtttc
     4921 cagtggtcga aaagcacggt ttcgtctgga tattcccggg cgacccggca ttgtctgata
     4981 ccgtggcacc gatggaaatg aacgaatggg atgatcttaa cgaaatcgcc agcttcgtgc
     5041 cgttgaggac ccgagctcat ttttcctacc tcatagaaaa tttgatggac atgtatcacg
     5101 gccgcttaca tgcgcagtat caggtatgga ccgccgagtc gctcaaggaa gtcgtggaag
     5161 acgaaaacca cgtctcagca acttaccaag ccacgactta ctaccaggta aaagatctcg
     5221 gctccattct ccagttgttc atcccttcac tgcgcaagct tcactcggtt ccgcttacgg
     5281 tcacctatga atatcccaac tggaagtcgg cgcttggaga agatttcaag atcttttgtc
     5341 tgatttgccc ggtgaatgaa cgattaaccg atgcctacct catccactac acatcgcttg
     5401 caaagttcaa aggcttgaat catgcgcctc tcgcggttcg acgccttttg aagcgcgctc
     5461 ttagcaatgt cgctaaaaaa ttgttggcaa atctagttcg tcaggacgtt gtaatgatcg
     5521 aggacgaaca agcagccttc gaccaggacc cgctgcgcca accgtttgaa gtcaaccgtg
     5581 ccatccgccg cgtacaaggt ttagttcgcc gccaggcaac tgaagaatcg ctccattgaa
     5641 gaattttcgg ggcgcgctct caggatttca catcgatgat taaaagcttt ctgcgctcga
     5701 agattaaaaa caaacttagc ggctatagca tcgttatcaa ccaacccgat ccgactaaac
     5761 ccaaattcgt ccaatcagcc aaaaaagttg ccgtggtagg cggcggtttg gcgggcatcg
     5821 gcgcggcctc cctgctcgcg gagcgaggat tcgaggtgac tttgttcgag cgaaatcatt
     5881 atctcggtgg gaaagtcggc tgctgggatg tttcgttcga aaacggcaac atgaccaagg
     5941 tggaccatgg cttccacgct tttttccggc actactacaa cttacgatcg ttcatggaaa
     6001 agatcggcgc agccgatcat ttgaaaacca tcgccgacta cctggttctg gccaaagacg
     6061 gaagacattt tagtttcaag aacgtcgagg ccacgccggt actaaatatt ctatcactgg
     6121 gcagaaacaa gtttttcaaa tttcgcgatg tcttgttcaa cccgccaagc tggagaatgg
     6181 gcgagtttct tgaatacgat caaacaaaaa cttttgctaa atatgactcc agcagtttcg
     6241 cagagttcgc aaaagaagcg agtttaccgg agtctctgca actgattttt aatacctttg
     6301 ccagggcttt cttcgccccg gccgacaagc tgagtacagc cgagttgatg aaaagctttc
     6361 attttttcta cctcagccac aatcatggcc tgctttatga ttatttcgac accgactatc
     6421 aagaggcatt aattcaaccg gcaacccagc atctcaatca acaccgcgtc tcgatcaaac
     6481 taaacaaaag tgtggaggaa acgggccgcg acaatgacag atttatcgta gaatcagagc
     6541 cgttcgacta cttgattcta gcgtccgatg tggtcggaac aaaaagtatt tgcgcaaatt
     6601 ctccgtggat caggacatct tcggaaaaga cctactcgga attgatgtcg ttgaaggctt
     6661 cgggcggcta cgccgtgtac aggatctggc taaacaaaag aaccggcgag gaattgccgg
     6721 tattcatcat caccgagaag catgaggttc tcgactcggt tactttctac caccaattcg
     6781 acagaagcgc ggccgaatgg gcgcagggtt ccggcggcgg cgtttacgag ctgcactgtt
     6841 atgcgctacc cttccgcgaa ctgtcggaga gagatgtgaa agccgccttt ttggccgaac
     6901 tccatttcta ttttccggaa cttcaagatg ccacgataat ctatgaccac cttcaactca
     6961 ggcgtgattt taccgcgttc tatacaaacc tgaacaagag cagaccggag ttcactaccg
     7021 caataccgaa cctctatctc gccggggatt gggtaaagac cggcattccg gcaatgctga
     7081 tggaaggagc ctacacctcc ggcgttctgt gcgccaacgg cattctggcc aagcacggcc
     7141 tccaagagga gccggtacga tcagtgccga ctcgcggaat ctttgcttag agcttcagct
     7201 aatcccggaa cttaaatgtc actgacctat ttggtgttaa atctctgcat catcgccgta
     7261 cctgtgtggt acacaccgga caagcgcacc gcctattatc gtcggttgcc ggcgctgggt
     7321 ttttccatcg cggtggtgag caccagctat ctgctgtggg atatattggt taccgcacgc
     7381 ggtgagtgga gcttcaacgg aaaatatcta acgggcgtgc agattttaaa cattcctctc
     7441 gaagaaattc tgttttttat cactgttccg tatagctgct tgttcatcta tgaggtcgtg
     7501 ctgtacgcaa cgaaagccag caaattcaga ctgccggcga gtgctatcat cgcattgatc
     7561 ctcgctttat tcgcggcgag tctcggcgtc tattcgcaag gatacaccgc caaggcatta
     7621 gcctcgtgcg gtgtttttct tacatccgca ctcttgcttg atcggccgct tatcgaaagc
     7681 aaacagtatt ggacctggct ggcgatctgt tacgtgccct tcctgatcat caatactgtg
     7741 ttgacggcgc ttcccgtagt cgagtacaac cccaaggcga tctggggatt gcgcgtcggc
     7801 acgattccgc tggaggattt cttttataac ttcgcaatgc tgtcctttta cctgctgttc
     7861 tatcgactgg caaaatcctg gacggctcga acggtgccgc tagtctgaag ccttatcgag
     7921 agggtcaacc gttccaagct tcggctcgca actatgttct tgtttttgat ttcaccagag
     7981 tccgggggcc gataatgcga atcgctcgtc attttagagc atgccgccca cgcagcttcc
     8041 gtcagcggca gctcattgta ggacggtaaa tctgatggat tgttgagccg ctactcccgc
     8101 cttgagccgt gcc
//
LOCUS       DASUVS010000465         3238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_862642, whole genome shotgun sequence.
ACCESSION   DASUVS010000465 DASUVS010000000
VERSION     DASUVS010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862642"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VF182_24470"
     CDS             <1..289
                     /locus_tag="VF182_24470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX6440295.1"
                     /translation="GELDDATIVTIEGLKIGPELHAIQKAFIENGGAQCGYCTPGMLM
                     MSKALLDENPAPTEEDIRFALSGNLCRCTGYAKIVQAVQDAAAMMRNKTVP"
     gene            376..2691
                     /locus_tag="VF182_24475"
     CDS             376..2691
                     /locus_tag="VF182_24475"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6440296.1"
                     /translation="MAELTVIGKPVTRVDANEKVTGEAVYGYDLVLPNMVYGKVLFSP
                     KAHARIKKINTEKAKSYPGVVAVVTGADAPWTHGESVKDKPFLAQDKVRYIGEPIAAV
                     AAEDEDTAQAAVKLIEVEYEDLPVYVDPEEAAKPDATAIHEDFPKYRKAEFIVPGANA
                     NVAEHFKLRTGDIEQGFKESDLVLEERYFVPMIQHAAMEPHSAHAQFDKESGRLTIWV
                     ANDAPFRALNELTEALEMPKEKIRLINPLQGGGFGSKGGLKVEPIAVALAFHTDGRPV
                     RVKFNREETFISTLTRHEAVVYCKSGVKKDGTLMAREMTIYWGAGAYAEKSPTVCIRG
                     SLPAPGPYRIPHVKVDGYAVYTNKPVAGSYRGYGIPQGAWAGEQQMDEIAKRLGMDPV
                     EIRMKNIFIEGDTSYWGEQLHAVGLKETLTKAIEAIDWGKKPQQTKPGVKVGKGFACI
                     QKPTRSPTTSAAGVIVDNKGDVTVLAGTVEIGQGCNTILSQVAAEELKVPMEKVHMHP
                     LDTDVIPYDASTTSSRSTYHMGNAVRGAAIHAREQIMEAASPMFEAKTDDLDCREGKV
                     FLKDQPQMALTLGEVVRRKFGAEGVVRGDSSYTYEIGKDLDLETGHSDHASAFYMYAT
                     QAAEVEVDEESGRIRILRMSAAHDVGKAINPLNCVAQIEGGVVMGIGSALHEEMVIDQ
                     TGKVRNPSFLDYHLVTSLDTPKMIPIIVECHEPEGPYGAKGLGEPGLAPTPAAIGNAV
                     ADALGVRIYDLPLKPENVYWAIQNKKKKDAA"
     gene            2879..>3238
                     /locus_tag="VF182_24480"
     CDS             2879..>3238
                     /locus_tag="VF182_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440297.1"
                     /translation="MAAQPPANGTPRGIQTVPGMPSVIDPNNIYSETRPDKLSAAVAK
                     HLSRVYVPNRSSNDVWVIDPTTDKVVDKFRVGVHPQHVVPSWDLKTLWVNNNAEGRDD
                     GSVTPIDPLTGKPGKNIP"
BASE COUNT          825 a          869 c          898 g          646 t
ORIGIN      
        1 cggcgaactc gacgatgcaa cgattgtcac aattgaaggc ctcaaaatcg gccccgagct
       61 tcacgccatt caaaaagcat tcattgaaaa tggcggcgcg caatgcggct attgcacgcc
      121 ggggatgtta atgatgtcca aagccctgct cgacgagaat cctgcaccga cggaagaaga
      181 cattcgcttc gctctctcgg gcaatctttg ccgttgcacg ggatacgcca agatcgttca
      241 agccgtgcaa gatgcggcag cgatgatgcg aaacaagact gttccatagc attcgagttg
      301 ttggtcgttc gaaggtgttg aaacggagca gcgattgaac ggtttgaatg tttagaactt
      361 ttaaggagaa gagaaatggc cgagcttaca gttattggga aaccagtcac ccgcgtcgac
      421 gcaaacgaaa aggtcacggg ggaagcggta tacggatatg atctggtcct gcccaatatg
      481 gtgtacggca aggtattatt cagtcccaag gcgcacgcca ggatcaagaa aatcaacacc
      541 gaaaaggcaa aaagctatcc tggtgtcgtc gccgtcgtca ccggtgcgga cgcgccgtgg
      601 acgcatggcg aaagcgtcaa ggataagccg ttcttagctc aggacaaagt tcggtacatc
      661 ggtgagccca tcgctgcggt tgctgcggaa gacgaggata ccgcgcaggc cgcagtaaag
      721 ctgatcgagg tggaatacga agacttgccg gtttacgtgg atccggagga agccgctaaa
      781 cctgatgcta ctgccattca cgaagatttt cccaaatacc gcaaagctga gtttatcgtt
      841 cccggcgcaa atgccaacgt cgctgaacac ttcaagcttc ggaccggcga tatcgagcaa
      901 ggcttcaaag aatcagatct cgtgttggaa gaaagatatt ttgtgccgat gatccaacat
      961 gccgccatgg aaccgcattc ggcccacgcg caatttgata aagaaagcgg tcgtctgacc
     1021 atttgggtcg ccaacgacgc gccctttcgc gcacttaatg aacttaccga agcgctggag
     1081 atgcccaaag aaaaaattcg ccttatcaac ccacttcagg gaggcggctt cggttccaaa
     1141 ggtggcttga aggtggaacc gatcgccgta gccctggcgt tccacaccga cggaagaccg
     1201 gtgcgggtga aatttaaccg cgaagaaact tttatctcga cgttaacccg gcacgaagca
     1261 gtcgtttact gcaagagcgg cgtcaaaaaa gacggcacac tgatggcgcg cgaaatgaca
     1321 atctactggg gcgctggtgc ctatgctgaa aagagcccga cggtttgcat ccgcggcagt
     1381 ttacccgcgc ccggtccgta ccgcatcccg cacgtcaaag tcgatggtta cgccgtttat
     1441 accaacaagc cggtggcagg ctcctaccgt ggctatggca tccctcaagg ggcctgggcg
     1501 ggcgagcaac agatggatga aattgccaaa cgcctcggca tggatccggt tgaaatccgc
     1561 atgaagaata tttttatcga aggcgacact tcgtactggg gcgagcagct ccatgcagtc
     1621 ggcttgaaag aaaccctgac caaagcgatt gaagccatcg attggggtaa gaagccacag
     1681 caaaccaagc cgggtgttaa agtcggcaag gggttcgcgt gcatccaaaa accgacgcgc
     1741 agcccaacga catcggcggc aggcgtcata gtggataaca aaggcgacgt cacggtgctt
     1801 gcgggcaccg tggagatcgg ccagggatgt aataccattc tttcacaagt agcggcggaa
     1861 gagctcaagg taccgatgga aaaagttcat atgcatccgc tcgacacaga tgtaattcct
     1921 tacgacgctt cgacgacctc cagtcgcagc acgtatcata tgggcaatgc cgtgcgtggc
     1981 gcggcgattc atgcgcgtga acagatcatg gaagccgcca gtccgatgtt cgaagcgaaa
     2041 acagatgatt tggattgccg agagggcaag gtgtttctca aggatcagcc gcaaatggct
     2101 ttgaccttgg gcgaagtggt acgccgcaaa ttcggcgccg aaggcgtcgt tcgcggcgac
     2161 tcttcttaca cttacgagat cggcaaggat ctcgatctcg aaaccggtca cagcgatcat
     2221 gcttcggctt tttacatgta cgccacgcaa gctgcggaag tcgaagtcga cgaagagagc
     2281 ggtcggataa gaattctccg aatgtccgcc gctcacgatg tcggcaaagc gatcaacccg
     2341 ctgaactgcg tcgctcagat cgaaggcggt gtggtcatgg gcatcggctc ggcgctgcac
     2401 gaggagatgg tcatcgatca aaccggaaag gtccgcaatc cttctttcct cgattatcac
     2461 ctcgttacat cactggacac tccgaaaatg attccgatca ttgttgaatg ccatgaaccc
     2521 gaaggacctt acggcgccaa agggttgggc gaacccgggc ttgcgcctac gcccgccgcc
     2581 atcggcaatg ctgtcgccga tgctttggga gtgcgcatct acgatctgcc gctcaaaccg
     2641 gagaacgtct actgggcaat ccagaataag aagaaaaaag acgccgcctg aagtctcaca
     2701 tcgtaatttt tttccttttc gtctaagata cggcattcca aggggcggga gtcatcagag
     2761 gctctcgtcc gttgactcac aactgccgga ggtcgctgtg atcggtacca aaaggaaaag
     2821 gctctacgcg atagttgcgc taacctttgc gctcttcccg tgcccctcat ttcatacgat
     2881 cgcggcgcaa ccacccgcaa acggaacccc gcgcgggatt caaactgtgc cgggaatgcc
     2941 ttcggtcatc gatccgaaca atatttacag cgaaacgcgg cctgacaagc tgagcgcggc
     3001 ggtggcaaaa cacctctcgc gcgtctatgt gccgaaccgc agttcaaacg acgtctgggt
     3061 aatcgatcca acgaccgata aagtcgtcga caaattccgc gtcggcgttc atccgcagca
     3121 cgttgttcct tcctgggatt tgaaaactct ctgggtcaat aataacgctg agggacgaga
     3181 cgacggcagc gtgacaccca tcgacccgtt aactggcaag ccgggaaaga acatcccc
//
LOCUS       DASUVS010000466         5745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1955976, whole genome shotgun sequence.
ACCESSION   DASUVS010000466 DASUVS010000000
VERSION     DASUVS010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1955976"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VF182_24485"
     CDS             complement(<1..264)
                     /locus_tag="VF182_24485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440298.1"
                     /translation="MREKIVFLRDTECVGFRVALSLCFLLILGGQTAQGAGGSGAATA
                     LTFSSEQNLGRIDKNPSTPFLRYSPDGRLHAIWSEDDETSWPRT"
     gene            complement(369..782)
                     /locus_tag="VF182_24490"
     CDS             complement(369..782)
                     /locus_tag="VF182_24490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440299.1"
                     /translation="MRRALLARRGRARLTLAAAFLFFLLYSAPHRVHHLFEELGVSRK
                     TSDDAAKATAPHDRPERNAEHDNPGHEHDHNSRGSAKADCTVQVVAQNFHVAPEQAIV
                     IGYRELESQTRPAVTLSWHYHFSPSPFSQRAPPRA"
     gene            complement(888..1628)
                     /locus_tag="VF182_24495"
     CDS             complement(888..1628)
                     /locus_tag="VF182_24495"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX6440300.1"
                     /translation="MGAKASKAGRPLVVDDAAPVAVGHVEVEIGLSHTRVHGGGRQQA
                     VPTLTTAYGIIDRLEIGLGIQRVNHDQGGDAPLRGFEDLHLLTKFTVIEETEVLPAAA
                     FSLDVSVPTANKAKGRSTGKSDQAFTFIMSKLHDLTGLHLNLGYLWVDSPRGAKLKNR
                     LRGGFAAEYGIHPGTALVGEVFGSSRASKDDQNEAAFQLGARYALTPGLVVDAAGGRS
                     LRSSGASVQVTLGLTWTIGIANYLKNGN"
     gene            complement(1761..2591)
                     /locus_tag="VF182_24500"
     CDS             complement(1761..2591)
                     /locus_tag="VF182_24500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX6440301.1"
                     /translation="MSLFIPFEYEFFRNGFIAAVLVGALCGFIGVYVVLRRMSYIGHG
                     LSHAIFGGAVLSYVMNINFFLGAGAWALLAGLLIHRIGRRRWVGADAAIGVVTTSSFA
                     LGVAIVSTYRSFTRNIEAALFGNILGITTRDLWIILAISLLVFCALFIMRRQLLFTTF
                     DPEVASAYGVSTDTIDRLFVVLLAVTVLASTQILGVTLVAAAMVTPPVIARYLTHKFH
                     TLLIVSPVLGAFCGAAGMFLSYYFDISSAATIVLTAATLFIAVELIIITRGRLSTVQR
                     "
     gene            complement(2687..3547)
                     /locus_tag="VF182_24505"
     CDS             complement(2687..3547)
                     /locus_tag="VF182_24505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX6440302.1"
                     /translation="MNPHDHRPGDPQSLHPPGVEERVAALSSTVPLIELVNVSSSYGL
                     TTALENVSLKIWPGQFMAIVGPNGGGKTTLLRTILHMLPVTSGKVFSRGRELKRSALQ
                     RIGYVPQLETIDWNFPITVEEVITMGFFNRQRWFGGTGENEKRKLRDIMERLNLTALG
                     RRHIRELSGGQQQAVFIGRALLGDPELILLDEPVSGLDMRSRDDVIHFLHEINHQGVA
                     IAITAHDLNWVAAHLPWVVCLNRRLIAEGQPQKIFNPTVLTETYSGEMVVFNHDGMVM
                     IGERPHAPHT"
     gene            complement(3560..4573)
                     /locus_tag="VF182_24510"
     CDS             complement(3560..4573)
                     /locus_tag="VF182_24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX6440303.1"
                     /translation="MRYKGKFPYLVVVVLACVITACSRQEDKQSVKPEKLNVVSTVAP
                     ITSIIENIGGDKIGLTGIIPEGINSHTFEPVPSDVKLVAQADLLVANGLDLEIPTLDL
                     ARANMKKGVALLLLGEKTISEKEYIYDFSFPKEHGHPNPHLWLNPQHAMRYATLIRDE
                     LIRLDSNNKSTYEANAAAFLKKVEALDSAIEESVRTIPESNRRLLTYHDSWPYFARRY
                     GFQIIGAVQPSDFSDPSPREVKRLIDQIRKEKVPAVFGSEVFPSPVLEQIAREGKTRY
                     IDKLRDDELPGRPGDARHSYLGMMIENLSIMFEALGGSAEAFKNVDTSPVAGSHGNYR
                     RKD"
     gene            complement(4949..5365)
                     /gene="nikR"
                     /locus_tag="VF182_24515"
     CDS             complement(4949..5365)
                     /gene="nikR"
                     /locus_tag="VF182_24515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HEX6440304.1"
                     /translation="MSTVSRFGVSLEEDLLAAFDLSIGGEGYQNRSEAIRDLIRDYLI
                     QKRVGQGDTEVIGVVTLLYDHRVHRLSDVLADMQHKAQVAVNASLHIHLDQHNCLEVI
                     VIRGRGDQVHEIAGKLIATKGVQNGKLVTTSPELKH"
     gene            5551..>5745
                     /locus_tag="VF182_24520"
     CDS             5551..>5745
                     /locus_tag="VF182_24520"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6440305.1"
                     /translation="MLPDIMLVILSHSTMETKAAGIQLENRLRDKRQALALSQKQLAE
                     MAGITRQAVSALEANQYSPAT"
BASE COUNT         1335 a         1568 c         1570 g         1272 t
ORIGIN      
        1 tgtccgcggc caggatgtct cgtcgtcttc gctccaaatt gcgtggagcc ggccgtcagg
       61 gctgtaacgt agaaatggag tggatgggtt tttgtcgatt cggcccaggt tttgttccga
      121 gctaaaggtt aaggctgtgg cggcacccga gccgccggct ccttgcgcag tttggccgcc
      181 aagaatgagc aaaaagcaca atgacaaggc gactcggaag ccaacgcact cggtgtcccg
      241 gagaaaaact atcttttctc tcatgtgacc tccacgttag acggaatacg ccagcaccac
      301 ggtattcccg agcgtgcggg attgaccgtc gtgatgaccg tcgactatcg gacgagagaa
      361 gctgcagtct aggcccgggg aggcgcgcgt tgggaaaaag gagaagggct gaaatgataa
      421 tgccacgaga gggtgacagc cggtcgggtt tgtgactcaa gttctcggta gccaattact
      481 atcgcttgct ccggcgcaac atgaaaattc tgagcgacca cctgcaccgt gcagtcagct
      541 ttggcggaac cgcgcgagtt atgatcgtgc tcatggccgg ggttgtcatg ctccgcgttt
      601 cgctcagggc gatcatgtgg cgcggttgcc ttagctgcat catcgctggt tttgcgtgaa
      661 acgccgagct cttcgaagag gtgatgcacc cgatgcggcg ccgaatagag taggaagaac
      721 aaaaaggcgg cggccagcgt aaggcgtgct cggccccgtc gggcgagtaa tgctcggcgc
      781 ataaaggctt gcatggctct tccacgccga accttaacgt attaacaatg ggtcaatcaa
      841 tcccgagctt gtggaggctc tgtgacgctg gcgcagtgcg ccctagttca gttcccgttt
      901 tttaggtaat tcgcaatgcc aatggtccag gttaggccca acgtgacttg aacgcttgcg
      961 cccgatgagc gcagacttcg accgccggcg gcatcgacca ccaaccctgg agtgagcgca
     1021 tatcgcgccc cgagctggaa ggccgcctcg ttttggtcgt ctttgcttgc gcgcgaagag
     1081 ccgaacactt caccgacgag ggcagtcccc ggatgaatgc cgtactcagc tgcgaacccg
     1141 ccgcgcagac gattcttgag ctttgcgccc cgtgggctat caacccaaag atatcccagg
     1201 ttcagatgca atcccgtcag atcatgaagt ttggacatga tgaaggtgaa ggcttgatct
     1261 gactttcccg ttgatcggcc tttagctttg tttgccgttg gtacactcac atctaatgaa
     1321 aatgcagcgg ccggtaaaac ttcagtttct tcgatgacgg tgaatttcgt caggagatga
     1381 agatcttcaa atcctcttag aggcgcgtct cctccttgat catgattgac ccgctgaatg
     1441 ccaagaccga tctccagcct gtctatgatg ccgtatgccg tcgttaacgt cggcaccgcc
     1501 tgttgacgcc cgcctccatg tacgcgggtg tgggataacc caatctccac ctcaacgtgc
     1561 cctacagcta ccggagccgc gtcgtccacc accaagggcc gacccgcctt agaagccttc
     1621 gcccccatca gaacacagct cgcgaccaag acaagtccaa gcaaccgtgg ccgtgctttg
     1681 cgcgcgcatc tccgggagat cgatgccgca cgcttgccgc atcggtcgtt tattcttgca
     1741 gtccagcttc ccaacatagc ttaccgttgt actgtcgata atcgtccacg cgtaatgatg
     1801 ataagttcca cggcaatgaa cagagtcgcc gccgtcaaca caatcgtggc tgccgaagaa
     1861 atatcgaagt agtaacttaa aaacatacct gcggcgccgc agaacgcgcc aagcaccggt
     1921 gacacaatga gcaatgtgtg gaacttgtgg gtgagatagc gggcgatcac ggggggagtg
     1981 accatagccg ccgcgaccag cgtaacaccg agtatttggg ttgacgctag aacggtgact
     2041 gcaagcagca ccacaaaaag ccggtcgatg gtgtccgtgg aaactccata ggctgaggcg
     2101 acttccggat cgaacgtcgt gaacagcagc tgacggcgca tgatgaaaag agcgcagaaa
     2161 acgagcagcg agatcgccag gatgatccag aggtcgcgcg ttgtaatgcc gaggatgttc
     2221 ccgaataacg cggcttcgat atttcgggtg aagctgcggt aggtcgaaac gatggcgacc
     2281 ccgagagcaa agcttgacgt tgtcaccaca ccgatagcgg cgtcagcgcc cacccaacgt
     2341 cgccggccga tgcgatgaat tagcaggccc gccaataacg cccaggctcc tgcgccgagg
     2401 aagaaattga tattcatgac ataactcagc acggcgccgc caaaaatcgc gtgggaaaga
     2461 ccgtggccga tatagctcat gcggcgcaac actacgtaaa ctccgataaa accacagagc
     2521 gcgccgacga ggaccgcggc tatgaagccg ttgcgaaaaa attcgtattc gaagggaatg
     2581 aataaagaca tgttccgatc gtttcaaagg tttctatctt ccagacgttg aattcttagg
     2641 acttgacgtc gttggaacgg ctggagcgtc ttgaatgggt cgttgattaa gtgtgcggcg
     2701 cgtgaggacg ttcgccgatc atgaccatgc cgtcatggtt gaacaccacc atctctccac
     2761 tgtatgtctc cgtcaaaacc gtggggttaa aaatcttctg cggctgccct tcagcaataa
     2821 gccggcgatt caggcacacc acccagggca ggtgagcggc aacccagttc agatcatgcg
     2881 cggtgatcgc aatggcgaca ccctggtgat tgatctcgtg cagaaagtga atcacatcgt
     2941 ctcgagaacg catatcgagg cctgagacgg gctcatcaag gaggatcaat tcgggatcac
     3001 cgaggagcgc gcgcccgata aacacagcct gctgctgccc gcccgatagc tcgcgaatat
     3061 ggcggcgacc gagggcagtc agattcagtc gttccataat gtcacgcagc ttgcgtttct
     3121 cgttctcccc cgtacctcca aaccaacgtt gccgattgaa gaaacccatc gtgataacct
     3181 cttcaaccgt gattggaaag ttccagtcaa ttgtttcaag ctgcggcaca tagcctatgc
     3241 gttgcagcgc cgaacgcttc aattcgcgac cgcggctgaa taccttcccg gacgtgaccg
     3301 gcagcatgtg caggatcgtc cgtaaaagag tcgttttgcc accgccgtta ggaccgacaa
     3361 tggccatgaa ttgtccaggc cagatcttga gcgagacgtt ttccagagcg gtagtcaacc
     3421 cataggagct gctgacattg accagttcaa tcaatggaac tgtgctggac aaggcagcaa
     3481 cgcgttcctc aacgcctgga ggatggagac tctgcggatc cccaggccgg tggtcgtgcg
     3541 ggttcatgtg gggactggtt taatcctttc tccggtaatt gccgtgggag cctgccaccg
     3601 gtgaggtatc gacgttcttg aacgcttcgg ctgaaccgcc gagcgcttcg aacataatgg
     3661 aaagattttc aatcatcata cctagataag aatgccgcgc atcgccaggc ctgccgggca
     3721 gttcatcgtc ccggagctta tcgatgtacc gggttttgcc ttcccgtgcg atctgttcga
     3781 gcacgggact gggaaaaact tccgagccga agacggccgg gactttttcc tttctgattt
     3841 gatcgataag cctctttact tccctaggac tgggatcgga gaaatccgac ggctgaacag
     3901 cgccgataat ctgaaagcca taacgccgcg cgaaatacgg ccacgaatcg tgatacgtga
     3961 gcaggcgccg gttactttcg ggtatcgtcc taaccgattc ctctattgcg ctgtcgagcg
     4021 cctcgacctt cttcaagaac gcagcggcat ttgcttcata ggtgctctta ttgttggaat
     4081 cgaggcgaat caactcgtcg cgaatcagag tagcgtaacg catggcgtgc tgagggttga
     4141 gccacaggtg cggatttgga tggccatgct ctttgggaaa actgaaatca taaatatatt
     4201 ctttttcgct gatcgtcttc tcgcccagaa gcagcagcgc gacgcctttt ttcatgttcg
     4261 ctcgcgctag atccagcgtc ggaatctcca gatctagtcc attggcgacg agcaggtccg
     4321 cctgcgcaac taacttgacg tccgatggca cgggctcaaa cgtatgagaa ttgattcctt
     4381 cgggaatgat tccggtgaga ccaattttat caccgccgat gttctcgatg atactggtga
     4441 tcggcgcgac ggtgctgacc acgtttagct tttcaggctt gacggattgt ttatcttcct
     4501 gtcggctgca ggcagtaata acgcacgcta agacgacaac aaccagataa gggaacttgc
     4561 ctttatatct catcgtcagc cttgtctatc tggagccgaa tcgtcaacat aggtgaactc
     4621 gcccgggaca ttcttgccgg cgttgtgagc aggctcgaaa cgggcctgct tcacccccct
     4681 cgagggcctc cgtcgaatgc ggtgccgagt gtcgcttctt tagtgatgcg acaacaacga
     4741 aacacgacgg gtgttgggcg gtccgtcgac cctggacata accgtccaga agaagaacgg
     4801 aaatcgctct gcgagccccg acaaaaatga agcaaactca cactggtgtt taccgacggc
     4861 gatgttgtga acatgctctc gtgatttcat ttcaaacgcc gtttcgtagc aacaaataca
     4921 aaaacgtgtc accttcttgt aaattttttt aatgcttcag ttccggcgaa gttgtgacca
     4981 gcttgccgtt ttgcacccct ttggtcgcta tcagtttacc cgcaatctcg tgcacctgat
     5041 cgccacgtcc gcggatgacg attacctcca gacaattatg ttggtccaaa tggatgtgca
     5101 ggctggcgtt gaccgcaacc tgcgccttgt gctgcatatc ggctaaaaca tcactcaacc
     5161 gatgcacgcg atggtcgtag agcagggtga ccacgccgat gacctccgtg tctccttggc
     5221 cgacgcgctt ctggatcaaa taatctcgaa tcaaatcgcg aattgcttcc gagcggtttt
     5281 gataaccctc gccgccgatg ctgagatcga aagcagcgag aagatcctcc tccaacgaaa
     5341 caccgaaacg ggagaccgtg ctcataactg gacttcgtaa cacgaagaaa gtttttgtgt
     5401 caacctggtg tgggcttggc tgaatctgcg gcgactagct aaaggtcgta aacaggagtg
     5461 ttgcttggcc gtcgctgatt gcgactaatg cgatttgttt gactgacttt tgggctcgta
     5521 agcgtcctcg attgacacac gaggcacgct atgttaccgg acataatgtt agttattctg
     5581 tcacatagca cgatggaaac caaagcagct ggcatccaat tggagaaccg cctgcgcgac
     5641 aaacgacagg cgttggctct ctcgcaaaaa caattagccg agatggcagg aataacgcgt
     5701 caagcggtta gcgctctaga ggcaaatcaa tactctcccg ctacc
//
LOCUS       DASUVS010000467         7512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_645266, whole genome shotgun sequence.
ACCESSION   DASUVS010000467 DASUVS010000000
VERSION     DASUVS010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7512
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645266"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VF182_24525"
     CDS             complement(<1..320)
                     /locus_tag="VF182_24525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6440306.1"
                     /translation="MTFFAKHLSVSIQRSAAEVYEFASDPKNLPQWAGGLSGSIKKID
                     DGWLAEAPMGTVKVKFAEINKFGVLDHEVTLASGIKFYNPMRILPNNDGSEVIFTLYR
                     QGDLS"
     gene            769..1185
                     /locus_tag="VF182_24530"
     CDS             769..1185
                     /locus_tag="VF182_24530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX6440307.1"
                     /translation="MSTQTKTECMLLFRGNDWHKGLSPEEIQEVVKQMYEWFERLTAQ
                     GKAKAGKPLFPEGKIVSQKKGRSVADGPFAESKEAIAGFFLLEVAGLEEAAEIAKDFP
                     GLNYGATVEVRPVAPECPLALSPREKSAAQPARASA"
     gene            1377..2747
                     /locus_tag="VF182_24535"
     CDS             1377..2747
                     /locus_tag="VF182_24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6440308.1"
                     /translation="MTTTRSDIKTPVPAPGEVTQVVEHCFRHESGKIISTLTRIFGIE
                     HINRAEDVVQETLARALQTWPYYGIPQNPAAWITQVAKNLALDLIRREKIFHNKEIEI
                     ARLLDQDSTDATAENAFKGEKEITDDRLRMMFVCCHPVLAQEAHVALALKTLCGFSPG
                     EIARAFLTSEAAIAKRLTRAKQKIRDARVPFEIPPGEELAQRLDGVLQTLYLLFNEGY
                     KASTGNQLIRETLCYEAIRLGGLLAEHPAGNRPRTHALLALMLLNGARLKARQDPEGN
                     ILRLQEQTRSSWDQAMIARGLYHLMQSMAADEISEYHLQAGIAACHCAAKDYESTDWP
                     QILSLYDRLLEMNDSPVILLNRAVALANVHGPKAGIEAVQTIQNRQELNSYYLLYAVL
                     GEFAADLNNKEAARDYFRKSLELATIKSEQIFMAKKLKQLDGGASNDTKPGRSRKGGG
                     VMTGKL"
     gene            complement(2776..3300)
                     /locus_tag="VF182_24540"
     CDS             complement(2776..3300)
                     /locus_tag="VF182_24540"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEX6440309.1"
                     /translation="MKCVRRENDQAHRTHRNLVRESGRTAMIGALLLMPLFSCAGKRP
                     TNIGIQASRLAACPSSPNCVSSDAADSAHSIAAFTLAIPPGEAWPALRRSVGGLRRTE
                     IIAETSDYLHAECTSAVFGFVDDLELHLRSAEGLIAVRSASRLGYSDLGVNRRRIEDL
                     RALLIKRGIVAALA"
     gene            complement(3308..3631)
                     /locus_tag="VF182_24545"
     CDS             complement(3308..3631)
                     /locus_tag="VF182_24545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HEX6440310.1"
                     /translation="MLRTSVGRLRMIGIVEGVSFLVLLGIAMPLKHFAQMPEAVRIVG
                     WMHGILFIGFVVALMLARDAMRWSFRWTGLVLLAALLPFGPFVIDGRLRKEDEGCGRA
                     EPPSI"
     gene            complement(3834..4721)
                     /locus_tag="VF182_24550"
     CDS             complement(3834..4721)
                     /locus_tag="VF182_24550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018502308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX6440311.1"
                     /translation="MKLGLQIPSFTWPTGTSGLASTLGRIGQAAEEAGFASLWVMDHF
                     FQIDMIGKPEEPMLEGYSALSFLAAVTRRIRLGTLVTGVIYREPGVLVKTITSLDVLS
                     GGRAYFGIGAGWYEREAVGLGVLFPPLKERFERLEETLQIALQMWSGKVGAHNGKHYR
                     LAETLNSPQPLSKPHPTILIGGAGEKKTLPLVAKYGDACNLYAFDNLELVRAKLEVLR
                     KNCDAIGRPYDEIERTAIGRLDVSSSRSVREAIEYCRSVNDAGIEHLIISVPNDYEIA
                     PIGLIGKQIIPAIADWPGR"
     gene            complement(4787..5920)
                     /locus_tag="VF182_24555"
     CDS             complement(4787..5920)
                     /locus_tag="VF182_24555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX6440312.1"
                     /translation="MLDHDTARINQLLGLMAQQSKEHAFVLLDANARVLWWSPGAGYI
                     FDRSSSEMVGEPLGILFTPEDRDRGIPDQEIQLAVNFGKAEDDRWQLRRDGSRFWATG
                     ILLALRDQHQNLVGFAKILRNRTDLREQVEALRNQVQALTGANQRKDNFLATFSHELR
                     NPMASICTAVELISRTASRNADLEGSLKLIEEQLEFVRRMLDDLLDVTRISTGKVRLN
                     MRPLVLQELIGRAVEAARPRIEERDQRIQVIGPSSSIVLQADPDRLQQVFSNLLSNAV
                     KYTPERGAICVEVTIEADEAVVRVSDTGVGIPHDMLPRIFELFTQVETQLSYGGLGIG
                     LALVKDFVALHGGSVQVRSEGVGKGSEFMVRLPLKVQGKNTEL"
     gene            6306..7295
                     /locus_tag="VF182_24560"
     CDS             6306..7295
                     /locus_tag="VF182_24560"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440313.1"
                     /translation="MIAKLAFIFGLLSSFVLMPGRQVCAASTSMRVLYPSFGGTWATP
                     WIAKEAGYFSAEGLDVELIRVGGSTRMVAAMLGGSAPIIQAGAAAAIAATAAGSDVVI
                     IAATGNVSPFRLMARPEIKQPTDLKGKRAGITTFGSTSDQVVRIALQKFNLEPNKDVA
                     ILSLGAQPEAFAALQSGAVQAAALSYPLYAKAAQLGMRELINFGDLGVEDVNGGVITT
                     RSYIAQHRDTATRFVRAYIRGAHRYRTDKEFSKRVLGKYGKITEDDLLEGTWQDYAPT
                     IQKVPRPSPKAIQFLIEFQFKGKTPLPKPENFVDMSIVDELEKNGFIDSVYKR"
BASE COUNT         1775 a         2101 c         2009 g         1627 t
ORIGIN      
        1 gacaggtcgc cttggcggta gagcgtaaag atcacttcgc tgccgtcgtt attcgggagg
       61 atacgcatgg gattataaaa cttgatccct gaggctaaag taacttcatg atccagaaca
      121 ccgaatttat ttatctcggc gaatttaact ttgactgtcc ccatcggcgc ttcggcgagc
      181 catccgtcgt cgatcttttt tatcgatccg ctgagtcctc cagcccattg cggaaggttc
      241 ttagggtcgg acgcaaactc gtaaacctca gccgcggagc gctggatcga cacactgaga
      301 tgcttggcaa agaacgtcat cagaatctcc tggggttaag cgaatgaagc cgatgttagc
      361 ttcgacggct taggttgacc gtcacatcgg ttcgcgtaat taatagatga tgagccaacc
      421 tgagcgcggt tttgcaagcc caatctcttt aacgtctgtt gaacaaaggt ttggctctgg
      481 cgggctcggc cctcggcgaa gtcacattta cctcttgggg tgcggggtcg caagacagat
      541 cacgtctttt ggactcggga tctgctcgaa gatagatgta tgaaagccgc gccagccgca
      601 actgactctt cacaaagaaa aaaagaagat tctagaatgc cattctggtt ttgtagcata
      661 gccgcgcgat gctgacctga tacatggaaa cgaaaaacgc ccaccattgt cctaaacgcc
      721 ctcgctcatt cgtcgactcg atgaaagcac aatctttagg aggaaattat gagcacacag
      781 accaaaacag aatgtatgtt gttattccgg ggcaacgact ggcacaaagg gctgtcaccc
      841 gaagagattc aagaagtggt caaacaaatg tacgaatggt tcgagcgctt gacggcgcag
      901 ggcaaggcca aagccggcaa accgctcttt ccagagggta aaatcgtttc gcagaaaaaa
      961 ggccgttccg tagccgatgg tccctttgcc gagtccaaag aagccatcgc cggattcttt
     1021 ctgctcgaag tcgccggttt agaggaagcg gcggagatcg ctaaggattt tccaggtttg
     1081 aattatggcg cgaccgtgga agtaaggccg gtggcgcctg aatgcccatt ggctctcagt
     1141 ccaagggaaa agagcgccgc acaaccggct cgcgccagcg cctgatcgca gcggtgtcga
     1201 gagggtttgt tgtacagtcc agctcagcgc gaccgtgagt tttgaagttc tttccgttcg
     1261 cggcgcgccg cagcagtcca tggaatcttc tcagcccggg tcgccttgaa tgcgcggttg
     1321 atccgaggtc tggcattggc catcgcggct gagacaatcg aacagatgca ccatctatga
     1381 caacgacgcg ttccgatatc aaaactccag tgccggcgcc tggcgaagtg acgcaggtgg
     1441 tggagcattg cttccggcat gagtcgggaa aaatcatctc gactttgacg agaatttttg
     1501 gcatcgaaca catcaaccga gccgaagacg tcgttcagga aacccttgcc agggccttac
     1561 agacctggcc gtattacggt atcccgcaga atcccgccgc ttggatcacg caggtagcga
     1621 aaaatctggc gctcgatctc atccgccgtg aaaagatttt tcataacaag gaaatcgaga
     1681 tcgcgcgctt gctggaccag gactcgacgg acgctacagc cgagaatgcc ttcaagggcg
     1741 aaaaggaaat caccgatgac cggctccgta tgatgtttgt ctgctgtcat cccgtgctgg
     1801 cgcaagaggc ccacgtagca ttggcgctca agactctttg cggctttagc cccggcgaga
     1861 ttgcccgggc atttcttacg tctgaagcgg cgattgccaa gcgcctgacc cgcgccaagc
     1921 aaaagatccg tgacgcgcgg gtgccgtttg aaattccccc gggggaggaa ctggcacagc
     1981 gcctcgacgg cgtcctgcag acgctctatc tgcttttcaa tgagggctac aaggcttcaa
     2041 caggtaatca actgatcaga gaaacgctct gttatgaagc gatccgcctg ggcgggttat
     2101 tggctgaaca tcccgcaggc aatcgtcctc gaactcacgc cctcctggcg ctgatgttat
     2161 tgaatggagc gcgattgaag gcgcggcagg acccggaggg aaacattctt cgactgcagg
     2221 agcagacccg ctcatcttgg gaccaggcga tgattgcccg ggggttgtat catctgatgc
     2281 aatccatggc cgccgacgaa atcagtgagt atcatcttca ggccgggatt gccgcctgcc
     2341 actgcgccgc caaggattac gaatccaccg attggccaca gatcctttcg ctttacgacc
     2401 ggctgcttga gatgaacgat tcgcccgtga ttctcctcaa tcgcgctgta gctctcgcta
     2461 acgtgcacgg tccgaaggca ggcatcgaag cggtgcaaac aattcaaaat cggcaagagt
     2521 tgaattctta ttatcttctt tatgcagttc tcggcgagtt cgcggcggat ctaaacaaca
     2581 aagaagcggc tcgcgattat ttccgtaagt ccctggaatt ggcgacgatc aagtcggaac
     2641 aaatcttcat ggcgaaaaaa ctaaagcagc ttgacggcgg agcctcgaac gataccaagc
     2701 ctgggcgaag ccgaaaaggc gggggcgtta tgaccggtaa actctagctg ccggctgatg
     2761 cgcgcaagcc atacgtcagg cgagcgcagc gacgatgcct cgctttatca atagcgcccg
     2821 cagatcttcg atccgccgcc gattgactcc tagatcactg tagccgagcc gcgatgccga
     2881 tctcaccgca ataagtcctt ccgcgctccg caagtgaagt tcgagatcgt cgacaaagcc
     2941 gaacacggca ctggtgcatt cggcgtgaag gtaatcggaa gtttcagcaa ttatttcagt
     3001 acgccgaaga cctccaacgg atcggcgtag cgcaggccat gcctcgcctg gcggaattgc
     3061 taaagtgaat gccgcaatag aatgcgccga atcagcggca tcgcttgaaa cgcaattggg
     3121 cgacgaagga caagccgcta agcgcgatgc ctggattccg atgttggtcg gtcgttttcc
     3181 ggcgcatgag aataacggca taagtaagag tgcgccgatc attgcagtac gaccgctctc
     3241 acggactaga ttcctgtgag tgcggtgggc ttgatcattt tctcgccgta cacacttcat
     3301 ttagagttca gatgcttggc ggctcagccc ggccgcatcc ttcgtcttct ttgcgcaagc
     3361 ggccatcgat cacgaacggc ccaaatggaa gcaaggccgc caggagtacg agacctgtcc
     3421 accgaaagct ccagcgcatg gcatcgcggg ccagcatcaa agcgacgaca aacccaataa
     3481 acagaatccc gtgcatccac ccgacgatac ggaccgcctc cggcatttga gcaaaatgct
     3541 ttaacggcat cgcgattccc agcagcacca ggaatgaaac gccctcgaca attccaatca
     3601 ttcgaagccg gcccacggat gttctcagca tactctgcgt tcctattcaa accgatgccg
     3661 ccgttctagc ggctcgatat ctttgtctat tctacgatgg tcgatattct caagtcaccg
     3721 tggtcttaaa tggaagagga agaagttgat tcatactgct gcggcccggc aaatcgcccg
     3781 cggagaaaat cgcgtgcatg gaagtgaagc tgcttgatgg gtctacttct ctcttatctg
     3841 ccgggccagt cagcgatcgc aggaatgatt tgcttgccga ttaaaccgat gggcgctatc
     3901 tcgtaatcgt taggcacgct gatgatcaaa tgctcgatgc cggcgtcatt gacggaccgg
     3961 cagtattcga tggcctcgcg cacgctgcgt gagctactga catcgagccg gccaatggcg
     4021 gtgcgttcga tctcatcata aggccgtccg attgcgtcgc aatttttgcg cagcacctcg
     4081 agcttggcgc gaacgagttc cagattgtca aacgcataga gattgcaagc atcgccgtat
     4141 tttgccacga gaggcagggt cttcttctca ccggcgccgc caatcaaaat agttggatgc
     4201 ggtttcgaaa gcggctgcgg cgagttaagt gtctcggcaa gccgataatg cttgccgtta
     4261 tgggcgccga cttttccaga ccacatctgc agagcgatct gcagggtttc ctctaaacgc
     4321 tcgaagcgct ccttcaacgg tggaaataaa actccaagtc cgacggcctc gcgttcgtac
     4381 caccccgcac cgatcccgaa atacgcgcgg cctccggaca ggacatcaag agaggtaatg
     4441 gttttgacga gaacgcccgg ctcgcggtaa atcaccccgg tcacgagtgt gccgagacgg
     4501 attcgccggg tcaccgcagc caaaaagctt agcgcgctat agccttctaa cattggctcc
     4561 tcgggcttgc cgatcatgtc gatttgaaag aaatgatcca tcacccagag acttgcaaag
     4621 cccgcttcct ccgctgcttg accgatccga cccaaagttg atgctaaacc cgaagttccc
     4681 gtgggccaag taaaagacgg tatctggagt cccaatttca tcatgccctc cgggcaaaag
     4741 ctgaaccggg ctcgcggcga ttatttcgcc agaacctcaa aggccttcac agctcggtgt
     4801 ttttgccctg aacttttagc ggtagccgca ccataaactc gctccctttg ccaacgccct
     4861 cgctgcgaac ctggaccgaa ccaccgtgga gcgccacaaa gtctttgacc aaggccaagc
     4921 cgatgccaag cccgccgtac gaaagctgag tttccacctg agtgaatagc tcgaatattc
     4981 tcggcaacat atcgtgcgga atgccgactc cggtgtcgct gacccggacc acagcctcat
     5041 ccgcctcgat agtcacctcg acacagatgg cgccgcgctc cggcgtgtac ttgaccgcat
     5101 tacttagtaa attcgagaag acctgctgca gtcgatcagg atcggcttgc agcacaatcg
     5161 acgacgacgg cccaatgacc tggattcgtt ggtcgcgttc ttcaatcctg ggccgcgccg
     5221 cctccacagc ccttccgata agttcctgaa gcaccaaagg tcgcatgttt aggcgaacct
     5281 tgccggtgct gatgcgcgtc acatccaata agtcatcaag catccggcgg acgaactcga
     5341 gttgctcctc gatgagcttg agcgaacctt ccagatcggc gttccgtgaa gccgtcctac
     5401 tgatgagttc taccgcggtg catatcgacg ccatcggatt acgaagctca tgcgaaaatg
     5461 tcgccaaaaa gttatccttt cgctgattcg cgccagtcag tgcctgcact tgattgcgca
     5521 atgcttcaac ttgctcgcgc agatccgttc ggttgcgcaa gatcttggca aagcccacga
     5581 gattctggtg ctgatcacgg agagctagta agatgccggt cgcccaaaat cgcgaaccgt
     5641 ctcggcgcaa ctgccatcga tcatcttccg ctttgccgaa attgaccgct agttggattt
     5701 cctgatcggg aattcccctg tccctatcct ccggggtaaa cagaattccg agaggctcgc
     5761 ctaccatctc gcttgagctg cgatcgaaga tatacccggc cccgggactc caccaaagca
     5821 cgcgcgcatt cgcatccaaa agcacaaagg catgctcttt cgactgctgc gccatgaggc
     5881 caagaagttg attgatccgc gcggtatcat ggtccaacat ttagtaggtt cctttgttga
     5941 agaagcgagc tggctttgtg gcggtgttgt caggagaccc gctgtgtaag atattactga
     6001 gagtataccg gagatctcac gcaactccag attcattgca aagaatctgc aagcgaccgg
     6061 ccagcccaat cctcgaaaaa tgcagctaac atccgttaaa acataccgct gcgatgtcaa
     6121 agatcgagta tcccgcgggc gcgaggatgg taccggttat cagaagaacc accggggagt
     6181 ttgcattgat tcccgccgaa ctgttaaatc gtcgctgtcg acaacccaca cacggaaaat
     6241 cgtcaagtaa aactcatacg cttcaactcg ttgagtgagt gaccattcct tggagataaa
     6301 tctatatgat agcgaagctc gcgtttatat tcggattgct gtcttcgttt gttttaatgc
     6361 caggcagaca agtttgtgcc gcatcgactt cgatgcgggt tctatacccc agctttggtg
     6421 gcacttgggc cacgccttgg atcgccaaag aggcaggcta tttttccgcc gaaggactcg
     6481 atgttgaact catccgggtc ggcggcagca ccaggatggt ggctgctatg ttgggcggga
     6541 gcgcgccgat tattcaagcg ggcgccgctg cggcgattgc cgcgacggcc gctggaagcg
     6601 atgtcgttat catcgccgcg accgggaacg tatcaccgtt tcgtttgatg gcgcgacccg
     6661 agatcaagca gcctacagat ctaaagggca aaagagccgg catcaccacg ttcggatcga
     6721 catccgatca ggtagtgcgc atagcgctac agaagttcaa tttggagcca aataaggatg
     6781 tggcgatttt atctttgggc gcccagccgg aagcgtttgc cgcattgcag agcggcgccg
     6841 tgcaagcggc ggcgctaagc tacccgctct acgccaaagc cgcccagtta ggaatgcgcg
     6901 agttgatcaa cttcggtgac cttggagtcg aagacgtcaa tggcggcgtg attaccaccc
     6961 gctcttatat tgcgcagcat cgtgataccg cgacccgctt cgtcagagct tacatacgcg
     7021 gcgcgcaccg ttatcgtacc gacaaggaat ttagcaaacg agttctaggc aagtacggca
     7081 agatcaccga agatgatctt ttggaaggca cgtggcagga ctacgcgccg actattcaaa
     7141 aagttccacg cccctcgccc aaggccattc agtttctgat tgaattccag ttcaagggca
     7201 agacaccgct gcccaaaccg gaaaactttg tcgatatgtc tatcgtggac gaactcgaga
     7261 agaatggttt tatagattca gtatacaaga gatagaacgg caaggagcca ttgccgcccg
     7321 agaaggcgac taactgacgc tcggcagaga caactaatcg gaagacgcgg ccccacgcac
     7381 caactcccgt gaggccggcc accgggtcgc tggcatccta acggttactt cttctgaacc
     7441 tggagattgt tggtaacttt ttttacgcca ccaacggccc gcgctacctc ttccgcccgg
     7501 gatctgtcct gc
//
LOCUS       DASUVS010000468         8967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_863517, whole genome shotgun sequence.
ACCESSION   DASUVS010000468 DASUVS010000000
VERSION     DASUVS010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8967
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863517"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VF182_24565"
     CDS             complement(<1..289)
                     /locus_tag="VF182_24565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEX6440314.1"
                     /translation="MPKNDLNGRLRRTAAILALTVIYFSAGIFGLSLASSNPNASAVW
                     PSSGIALAAILLWGFKLWPGILLGAFLVNFATQGSIWTAIGIAAGNTAEALL"
     gene            complement(428..1351)
                     /locus_tag="VF182_24570"
     CDS             complement(428..1351)
                     /locus_tag="VF182_24570"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6440315.1"
                     /translation="MAVDSAAQATQKNKGPLVWLDMDQKELDDAYNQSVYAPNQEQIA
                     KRRRLASEAAIKRLAPALRFSYGPTDIEKLDIYRTTRPNAPIMIFTHGGAWRNGRAKD
                     FAFPAEVFINAGAHYIVLDFAAVHDVGGNLMVMADQVRRAVAWVYKNAETFGGNRNRL
                     YVAGHSSGAHLTGVILVTDWRRDFDLPADIIKGGLCVSGMYDLKPVRLSARSKYVKFD
                     DLTEEALSSQRHLESLNCPITVGYGSQETPEFQRQGREFAAAVKAAGKPVQFFVGEGF
                     NHFEMQETFGNPYGLAGRAALELMDLQVRYS"
     gene            complement(1651..1989)
                     /locus_tag="VF182_24575"
     CDS             complement(1651..1989)
                     /locus_tag="VF182_24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440316.1"
                     /translation="MPEQITKYPDVTLQVLREAGAVCGGGAPQKILKQCPAESFCSLP
                     TGEMCIYGIKDIPRMTQIAPAELAQVVCPLTPKGASFAAGFSGLEAAMIGAVFVGGLA
                     LGKIWRSSRN"
     gene            complement(2295..3644)
                     /locus_tag="VF182_24580"
     CDS             complement(2295..3644)
                     /locus_tag="VF182_24580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX6440317.1"
                     /translation="MMKNLRLKSTIALSLALSACSLAPQYQRPQMTMPAGWTEVDGVG
                     VSDHPATVPFWQELGSEELNQIMKIALAQNLDLEAALHRIDQARAQTKIAGSALFPQI
                     EATGGASRSFQDPRNVSIARGTGSISYEADLWGRNRSNRTAANYRAEATAFDRDALQL
                     VVTSDTADLYSQVLSLNERIRIAESNLKNAEEVLRIIEARYKAGSVSGLEVSQQRVAV
                     NNFRASLASLNQQRSTTLNALAILLGQAPQNLQAPRETLASLKVPDVNLTPPATLLTA
                     RPDIERAEASLRAANADIGAARAAFFPSLNFGLDASIAAGFGGPAATVVSLASSALAP
                     IFTGGRLTGNLEDVTARQKELAAQYQKTVLVAFQEVEDALAAYKNSNEQSVIARESVK
                     ESQAAYDIAKARFDAGAIDYLNLLVTQRSLYEAQDNQIAVYQDQIASFVQLRKALGA"
     gene            complement(3647..5587)
                     /locus_tag="VF182_24585"
     CDS             complement(3647..5587)
                     /locus_tag="VF182_24585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEX6440318.1"
                     /translation="MNDVILDLTAIEKHYQNGDTIVRALDAVSLKIHTGEFIAIMGQS
                     GSGKSTLMNIIGCLDRPTSGSYRVLGKEAANLSPDELAALRRETFGFVFQRYNLLATA
                     TAGENVEIPSVYAGLPKQKRSQRAKGLLQRLGLGDRTDHRPAELSGGQQQRVAIARAL
                     VNDPPVILADEPTGALDSKSGDEVLSLLKQLHAEGRTIILITHAENVAQHASRIVRIQ
                     DGRILDDSGVVRKANVMPVDPADLNLRSAVTLAASMQEALVTAWRSLRVNLFRTVLTL
                     LGIIIGVAAVVAMLAVGEGSRQKVLDRISSFGTNLMLIRPGAAGIRNTGDIVTLIPED
                     AAAIKALPNIETALPERSGRMTVRFGNRDYQTSVQGTGEDFPQARDWKVAQGQFFNAD
                     DMKQYAPVVVLGRTVATTLFPDGGEAVGNYVLLKNVPFLVIGVMTEKGASPNGSDQDD
                     VIFTPISTGLVRLFGKSYLSSITIKVSDAGDIAATQERVENLLRERHRTEDFSVRNMA
                     SYLQAAIETQDTFTLLLGTVAAISLLVGGIGVMNIMLVSVVERTREIGIRMATGARMR
                     DILLQFNIEAAVVCAAGGLLGILVGVAAGMILRYSGMSVIFSLTPALLAFACASATGL
                     IFGYLPARKAARLDPVVALASE"
     gene            complement(5718..6938)
                     /locus_tag="VF182_24590"
     CDS             complement(5718..6938)
                     /locus_tag="VF182_24590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX6440319.1"
                     /translation="MKWMKTIKQRPKKLITLLAVSGLIGGGWYWYASAARQPEQNAQQ
                     PITITRGTIQEVVTAQGKLESKQYVDVGTQVSGQIKKLHADIGDTVKQGQLLAELDPR
                     VYQAQVEANEARLNSLRAQLNQQKAQLTLSEQNLKRNRDLFGVNAVSQQALQESETEA
                     AVARAQVASIQAQIQETESNIKGNQTNLSFTKIYAPMSGTVTTRPAREGQTLNANQSP
                     PTILQIADLDTMTVRAQVAEADVTKLKEGMPVYFTTLGNTERRWHGTVRQILPAPQVV
                     NEVVLYDVLIDVKNEGRRLMTGMTTQVFFIFGKAEDVAIAPAEVLTRPLPEQDNDSGK
                     AYRITVLTENGREQRVIHVGLQTRTQAEVTAGLNPGERILVSRPQGQGTATAQNSRPR
                     AGNNTPRLNRGPQL"
     gene            complement(7055..7489)
                     /locus_tag="VF182_24595"
     CDS             complement(7055..7489)
                     /locus_tag="VF182_24595"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEX6440320.1"
                     /translation="MNTKIKSAFIISLVLNVLLLGIFFGTLSPGFGRESSRRERLGAE
                     IEKLPEPARSRFRDKMAQLRSADDPLREQMRQARNQAIELLVAEPFDEAAYDGQLVKI
                     NQLRGQMSKRVADDLKQLMRGLPPDQRAAVGDVLKRPAPSPG"
     gene            complement(7486..7797)
                     /locus_tag="VF182_24600"
     CDS             complement(7486..7797)
                     /locus_tag="VF182_24600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440321.1"
                     /translation="MAPEEDKIIDEMLNARRIRPASPDLAQRIILKAQMTPQIQVFSL
                     ARWIKQTFAELHLPNPAYVLAGTLMMGFVLGLNFPSDTVTDNADAVQALSFVDPDEGL
                     L"
     gene            complement(7806..8363)
                     /locus_tag="VF182_24605"
     CDS             complement(7806..8363)
                     /locus_tag="VF182_24605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6440322.1"
                     /translation="MPVPWLEKDDEELLALIQHGSQEAFSVLVQRHTERFYRLAYRYL
                     QSKEAAEDVVQDAFVKLWEDPGRWQAERNNKFTTWFYRVVVNLCLDWRKKKKPLELGD
                     HEPAIADDRISPDIAMIHAQEQKILEQEIAALPERQRIALNLCFDEGLSNQEAAEVMG
                     LKLKALQSLVMRAKTTLKERLKNHL"
BASE COUNT         1949 a         2493 c         2480 g         2045 t
ORIGIN      
        1 ccagaagcgc ttcagcggta ttgcctgcgg cgatgccgat cgccgtccag atcgaccctt
       61 gggtggcgaa gtttacaaga aatgcgccca gtagaatgcc cggccaaagc ttgaaacccc
      121 agaggagaat cgctgccagc gcaataccgc tcgacggcca gaccgccgaa gcattgggat
      181 tactagaagc tagggataag ccaaagattc cggcagaaaa atagatgacg gtgagcgcga
      241 ggatcgcagc tgtccggcgt aatctgccat tgagatcgtt cttaggcatc gacggatctt
      301 actagcttcg gacctgagcc tatgtgggaa cttgggcttg cctatgatac cggatcagat
      361 ggttttaatg aacacctctt gcgaaaatat tggcgacgga agccatcccg gtgagcgaag
      421 atcgccgtta cgaataccgc acctgcaaat ccatcagttc cagcgcggca cggccagcca
      481 gtccataggg attgccgaac gtctcttgca tctcgaaatg attgaagcct tcgccgacga
      541 agaactgcac aggcttaccg gcagctttga ccgccgccgc aaactcgcgt ccctgacgct
      601 ggaactccgg tgtctcctgc gaaccgtagc caactgtaat cggacagttt aaagattcga
      661 gatgtcgttg agagctgagc gcttcttcgg tgaggtcgtc gaattttaca tatttgctac
      721 gcgctgacag ccgtaccggt tttaaatcat acatgccgct gacacataat ccgcctttga
      781 taatgtcagc cggtaaatcg aagtcccggc gccaatctgt caccaggatc acgccggtga
      841 gatgcgcgcc cgaggagtgg cctgctacat aaaggcggtt gcgattgccg ccgaaggttt
      901 cggcgttctt gtacacccat gcgacagcgc gccgcacctg atccgccatc accataagat
      961 tgccgccgac gtcgtgtact gcggcaaaat cgaggacgat gtaatgagca ccggcgttga
     1021 taaaaacctc ggctgggaaa gcaaagtctt ttgcccgtcc gttgcgccag gcgccgccgt
     1081 gagtaaagat catgatcggc gcgttgggcc gcgtggtcct gtagatgtca agtttttcga
     1141 tgtcagttgg gccgtaggag aaacgcaagg cgggcgcaag gcgtttgatt gcggcttcgc
     1201 ttgctaatcg ccgccgcttg gcaatctgct cctgattagg cgcatagacc gattggttgt
     1261 acgcgtcatc gagttctttc tggtccatgt caagccagac cagcggccct ttattttttt
     1321 gagtcgcttg agcggcggaa tcaacagcca tgcttaatcc tccaatcgga agttggtgct
     1381 gccgacgata ggacaaatct attcgccgtt caaatagttc aagcagctcg aatgttcaaa
     1441 tagccatacg gcacctgcaa gccgcaaggg cttagcagtc catatttggg caattgccaa
     1501 aacaacaggc gaaaaaactt gaaaggtggt gctgaaataa aggtagatct tgcaagcggc
     1561 acggattagc cgcggcaaag cacatccgag gttttctcgg tttgaacggg cttgaaccgc
     1621 ttgaacgatt ggaacagtca gggatcgcgc tcaattgcgc gagcttcgcc agatctttcc
     1681 aagcgcgagc ccgccaacga acacggcgcc gatcatcgcc gcttccagcc ctgaaaatcc
     1741 cgctgcaaag cttgcacctt tgggtgttag cggacaaaca acctgagcca gttcggccgg
     1801 ggcgatctgg gtcattcgcg gaatgtcttt gatgccatag atgcacatct ctcctgtcgg
     1861 cagggagcag aagctttcgg ctggacattg cttgagaatt ttctgcggcg cccctcctcc
     1921 gcaaacagca ccggcttctc ttaaaacctg caacgtcacg tcgggatact tcgtgatttg
     1981 ctccggcata cggacctcct tcgtcggatt ggttgcaaac gttcagagag ttcaaatcgt
     2041 tcagcccagt caagcgcaaa tgccatcaat cgcttggcct aagtaatcat accattgtcg
     2101 gctacgaagt tgagacagag gcgagattga agttcacgtt cgagtttgac accggtctgt
     2161 atgactttta gttcattatg tcgaatgaaa aactgcgaag actcgttgac aggaactgtc
     2221 ttcggcagtg agagctgaac gagtgcaaca tttggaacgg ttgaacgatt ggaacgaatt
     2281 ccgcgttgac gctgttatgc gcctaatgct tttctgagtt gcacaaacga agcgatctga
     2341 tcctgataaa cggcaatctg gttatcctgt gcttcataca agctgcgttg agttaccagc
     2401 aaattcaaat aatcaatggc gccggcatca aagcgtgctt tagcgatgtc gtaggccgct
     2461 tgggattctt tgacggattc gcgtgcgatc acggattgct cattggagtt cttgtaggcg
     2521 gcgagcgcgt cttcgacttc ttgaaacgcc accagcacgg ttttttgata ctgcgccgcc
     2581 aactctttct ggcgcgccgt gacgtcttcg agattgcccg tgagtcggcc gccggtgaag
     2641 atcggcgcaa gcgcgctgct ggccagcgat actaccgtag ccgcgggtcc gccaaatccc
     2701 gccgcgatgc tggcgtccaa gccaaagttc aaggagggaa agaaagccgc gcgagcggcg
     2761 ccgatgtcag cattagcagc ccgcaggctt gcttcggctc gctcgatatc cgggcgcgca
     2821 gtaaggagcg ttgccggcgg ggtcaggttt acgtctggga ctttgagcga tgcgagcgtc
     2881 tcccgaggtg cttggagatt ctgcggcgcc tgccccaata gtatagccaa agcgttcaag
     2941 gtcgtgctgc gctgttgatt gagcgaagcc agcgaagcgc ggaaattgtt cacggcaacc
     3001 cgctgctgtg agacctccaa gccagagacg ctgccggcct tatagcgcgc ttcgataatc
     3061 cgcaggactt cttccgcatt cttcaaattg ctttcggcga tgcgaatgcg ttcgttcaag
     3121 ctaagcacct ggctgtaaag atcggcagtg tccgaggtga cgacgagctg gagcgcatcg
     3181 cggtcgaatg cagtggcctc ggcccgataa ttcgcggctg ttctgttgct gcgattcctg
     3241 ccccagagat cggcttcata actgatactg ccggtgccgc gcgctatgct gacatttctc
     3301 gggtcttgaa aactgcgcga cgcgccgcca gtggcttcga tctgaggaaa gagcgccgag
     3361 cccgctatct tggtttgagc acgcgcctga tcgatacgat gaagcgctgc ctcgagatcc
     3421 agattctgcg ccagcgcaat tttcataatc tgattcagtt cttcactgcc aagctcctgc
     3481 caaaacggta cggtcgccgg gtgatcgctg accccaaccc cgtcaacctc ggtccagccc
     3541 gccggcatcg tcatctgcgg acgctggtat tgcggcgcca aactgcacgc cgacagagct
     3601 aacgaaagag cgattgtgct tttaagtcta agattcttca tcatactcac tccgaagcca
     3661 aagcgaccac cggatccagc cgcgccgcct tacgtgccgg caagtaaccg aagatcaagc
     3721 cggttgccga agcgcaggcg aaggcgagca gcgcgggtgt gagcgaaaaa atcacgctca
     3781 tgccgctgta acgcagaatc atgccggcag cgacgccaac gagaattcct aacaatccgc
     3841 cagcggcgca aactaccgcc gcttcgatgt tgaactgcag caggatgtcg cgcattcgcg
     3901 cgccggtcgc catccggatg ccgatctcgc gggtgcgctc aacaacgctg accagcatga
     3961 tgttcatgac tccgatgccg ccgactagca gtgaaatcgc cgccacggtg ccgaggagca
     4021 gcgtgaacgt atcctgagtc tcgatggcag cttgtagata cgacgccatg ttgcgcacac
     4081 tgaaatcctc ggtgcgatgg cgctcgcgca ataggttttc gacccgctct tgcgttgccg
     4141 cgatgtcgcc cgcatcagag actttaatgg taatgctgct caaataactc ttgccgaaca
     4201 atcgcaccaa accggtgctg atcggcgtga agataacgtc atcctgatcg ctgccgttgg
     4261 gagaggcgcc tttttcggtc atgacgccga tcaccagaaa gggcacgttc ttcaaaagaa
     4321 cgtaattccc aaccgcttca ccgccatccg gaaaaagcgt agtcgccacc gtgcggccga
     4381 gcacaaccac cggcgcatac tgcttcatat catcggcgtt gaagaattgc ccttgggcga
     4441 ccttccagtc gcgcgcttgc ggaaagtctt cgccggttcc ttggaccgat gtttgataat
     4501 cgcggttgcc gaatcgaacg gtcatgcgcc cgctgcgctc gggtagtgcg gtctcgatat
     4561 tcggcaatgc tttgatggcg gcggcatcct cgggaatcag agtgacgata tcgccggtgt
     4621 tacgaatgcc ggcagcacct gggcggatca gcatcagatt ggttccgaac gagctgatgc
     4681 ggtcgagcac cttttgccgg ctgccttcgc cgacggcaag catcgcgacg accgcggcga
     4741 cgccgataat gatgccgagc aacgtcaaca ccgtgcgaaa aagattgacc cgcaacgagc
     4801 gccaggcggt gaccaaagcc tcctgcatgc tcgccgccag agtgaccgcg ctcctaagat
     4861 tgagatcggc ggggtcaacc ggcatcacat tcgcttttct gacaaccccg ctgtcatcga
     4921 ggattcgacc atcttgaatg cgcacgatgc ggctggcgtg ctgcgctaca ttttcggcgt
     4981 gggtgatgag aatgatggta cggccttcgg catgcagctg tttgagcagc gacaaaactt
     5041 catcgccgct tttgctgtca agagccccgg tgggttcatc ggcgagaatc accggtgggt
     5101 cgtttaccag cgctcgcgcg atggcgaccc gttgctgctg gccgccggat agctccgccg
     5161 gccgatgatc ggtgcgatcg ccaagtccga gccgctgcag caaccccttg gcgcgctgcg
     5221 atcttttctg tttaggcaat cccgcgtaaa cggatggaat ctccacgttc tcgccggctg
     5281 tggcagtcgc cagcaagtta taacgttgaa atacgaagcc aaaggtttcg cgccgcaaag
     5341 ccgccagttc gtcgggcgag agattagcgg cctctttacc gagcacccga taactcccgg
     5401 aagtcggccg atcgagacaa ccgataatgt tcatcaaggt cgacttaccc gagccggact
     5461 ggcccatgat cgcgataaat tcgccggtgt ggattttcaa cgacacagcg tcgagagcgc
     5521 gaacgatggt atcgccgttc tgataatgtt tctcgattgc ggttaaatcc aaaatgacgt
     5581 cgttcatagt tgcggccctg tttgaaaatc tcgcaggtgt tgcgaccggc aaaagaatgc
     5641 agaatcgcat cgtcgattgc ctcgttgaaa aattgcatgt gccgcgagag gccaacaaaa
     5701 tgtcaaagtg agcatgttca tagttgcggc ccccgattaa gtctaggcgt attgttgccc
     5761 gctcgcggcc gactgttctg cgcggtagcg gtgccctgcc cttggggccg gctgaccagg
     5821 atacgttcgc ccggattcaa accggcggtg acttccgcct gggtgcgcgt ctgcagaccg
     5881 acgtgaatga cacgttgctc acggccgttc tcggtgagca cggtaatccg gtaagccttg
     5941 ccggaatcat tgtcctgctc ggggagcggg cgggtcagca cctccgcggg agctatcgcg
     6001 acgtcttcgg ctttgccgaa tatgaaaaag acctgtgtgg tcatgccggt catgagccgg
     6061 cggccttcgt tcttgacgtc gatcaaaaca tcgtaaagca caacttcatt gactacctgc
     6121 ggcgcaggaa gaatttgccg cactgtccca tgccatcgac gctcggtatt tcctagagtt
     6181 gtgaaataaa caggcatccc ttccttgagc tttgtgacat cggcttcggc gacttgtgcc
     6241 cgcacggtca tggtgtccag atcggcgatc tgcaaaatcg tcggcgggct ctggttggcg
     6301 ttcaaggtct ggccttcgcg cgccggtcga gtcgtcacgg tgccggacat gggggcatag
     6361 attttggtga agctaaggtt cgtctgattg cccttgatgt ttgattcggt ttcctggatt
     6421 tgtgcctgaa tcgaagcaac ttgcgctcta gccaccgccg cttctgtttc ggattcctga
     6481 agcgcctgct ggcttacggc attgacgccg aagagatcgc gattgcgctt gaggttctgc
     6541 tccgacaacg tgagctgagc tttctgctgg ttcagttgag ctctcaggct gttcagccgg
     6601 gcttcgttgg cttcaacttg tgcctgatac acccgcggat caagctccgc gagcagttgg
     6661 ccctgcttca ccgtatcacc gatgtctgcg tgaagttttt taatttgacc ggatacttgc
     6721 gtgccgacgt cgacgtattg ctttgattct agcttgcctt gcgccgtgac cacttcctga
     6781 atcgtcccgc gggttattgt gatcggctgt tgagcatttt gctccggctg gcgtgctgca
     6841 ctggcatacc aataccagcc cccgccgatc aaccccgata cagcaagcaa agtgatcagc
     6901 tttttcgggc gctgtttgat tgttttcatc catttcatag tggcgttgcc tttcgttgtc
     6961 cgtaagttga tacgaatcaa gatgcctttg tctccgccat tcaggcaaaa aaaacgccgg
     7021 tgagaccggc gctttctggc aatttaggaa gcggttaccc gggtgaaggc gcgggccgct
     7081 tgagcacgtc accgaccgcg gcacgctggt ccggcggcaa tcctctcatg agctgcttaa
     7141 ggtcgtcagc cactcgtttc gacatctgcc cacgcagttg attgatcttg accagttgac
     7201 cgtcgtaagc cgcctcatcg aacggttcgg caaccagaag ctcgatcgct tgattgcggg
     7261 cctgccgcat ctgctcgcgc aacggatcat ctgccgagcg aagctgagcc atcttatcgc
     7321 ggaatcggct gcgcgccggt tcaggcaatt tttcgatttc cgcaccgagc cgctctcgcc
     7381 gtgaactctc ccgcccgaag ccggggctta atgtgccgaa gaaaatgccc agcaacaaaa
     7441 cattcaagac cagcgagatg ataaaggcgc tttttatctt cgtattcata agagtccttc
     7501 atcgggatcg acgaagctca acgcctgaac ggcatcggcg ttgtcggtta cggtatcgga
     7561 ggggaaattc aagccgagaa caaatcccat cattaacgtg cccgccagga cataagccgg
     7621 attcggtaaa tgtagttcgg cgaaagtttg cttgatccat cgcgccaacg aaaatacctg
     7681 tatttgaggc gtcatctgcg ccttcaagat aatccgctgg gctaggtccg ggcttgcggg
     7741 ccggatgcgg cgcgcattaa gcatctcgtc gattattttg tcttcctcgg gggccatact
     7801 aaccctcaca aatggttttt caggcgttct ttaagcgtcg tcttggctcg cataaccagc
     7861 gattgcaaag ccttgagctt gagccccatc acttcggcgg cttcctgatt gctcaatcct
     7921 tcatcgaaac agagattcaa tgcaatccgc tgccgctctg gcagagccgc gatttcctgc
     7981 tcaagtattt tctgttcctg cgcgtgtatc atagcgatat cggggctgat ccggtcatca
     8041 gcgattgcag gctcgtgatc gccaagctcg agtggttttt tctttttccg ccagtcgaga
     8101 cacagattaa ccacgacccg gtaaaaccaa gtggtgaatt tgttatttcg ctcggcttgc
     8161 caccggcccg gatcctccca caatttaacg aacgcatcct gcacgacgtc ttcggcagcc
     8221 tccttacttt gtagatagcg gtaggcaagc cggtaaaatc tctcagtgtg ccgctgtacg
     8281 agaacactga acgcctcctg gctgccatgt tggatgagcg caaggagctc ttcatcgtct
     8341 ttttcgagcc atggaaccgg catagagttg ggattcgagt tgtctctcga acttcagtga
     8401 attgtaggaa tttctcaatc atacatggat ccaactgaga cccaaagtgc cgattaacat
     8461 tacgcctcaa ctgatgatac gaccgaggtg ccaagagtct tcggcggcgg tgagttttat
     8521 tagaaatggt ccagtgaatc gtagtttgct catgctgccc ccgtcaactc ggggcccgct
     8581 tcgaatgtcc agtcggcatt cccggttgcc tttaacgttc aaacacattc cgacctcgaa
     8641 gcaacctaaa atcttgggcg agcttgacag ccgccgaacc gatgccttag aagcagagaa
     8701 gcaacggttg cgtgagctat tgaagtctaa gggtcgttga tttgatagac caaaagcagc
     8761 ctgaaaacat atgaacagaa aaggagacaa atcatggcac gcatcgcaca cattgcgctt
     8821 ctgacaaaag ataccgagag attggcggag ttctataaga agtcgttcgg aatgaaagaa
     8881 gttgcacgca gcggcgaagg cggggagcat gggaaagcca tctatgtatc cgacggccac
     8941 atcaatctcg ccattctgcc ggcgcgc
//
LOCUS       DASUVS010000469        24376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1302399, whole genome shotgun sequence.
ACCESSION   DASUVS010000469 DASUVS010000000
VERSION     DASUVS010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 24376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24376
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302399"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(730..936)
                     /locus_tag="VF182_24610"
     CDS             complement(730..936)
                     /locus_tag="VF182_24610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440323.1"
                     /translation="MPGADNAPRQAEENQAEHRVAAGNVHEPPGLALRADPSNGKTYD
                     KLPVAFYHIGMRGFDTKPLLDLFN"
     gene            935..1888
                     /locus_tag="VF182_24615"
     CDS             935..1888
                     /locus_tag="VF182_24615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6440324.1"
                     /translation="MRASHLLCAAALVILRYARSLVTLRPQPYAGHNPLGALAIIVLL
                     GLVLITVATGHLVYNDTAGEWAEEMHEVAARRHARGGHRTHHLSDGNQLSAPRKSCRR
                     DAHRTQGCPSGSRNSMVVPMVGGGDAHPRAGVLVDAVAKRGSNRERSFAAGNERSIGL
                     KEGLTATRILLAEDDQILGSGLKAGLGQAGFQVDWVRDGQGAQKQIEDEEYDAVVLDL
                     GLLLKDGLDVLRQARKRGKALPVLVLTALVLSLGWTYFCRAARPYPGARTHARDQKPG
                     RTKQTRRKIIRQTAYRSNEDSDNDSANVGKEKIFESPEVNR"
     gene            complement(2187..3314)
                     /locus_tag="VF182_24620"
     CDS             complement(2187..3314)
                     /locus_tag="VF182_24620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440325.1"
                     /translation="MGRVYELVREVVQGTLTQTTPDTTVSRQAAGNEDSLCDATEELD
                     KIFADGIRRLKAAVSDNQAIALSKTKHAEEVIEGLKANISELEARVRETEDMVHSQNI
                     AGKKTEESLRTEIDNLQHALKEKEAALQSRTSEVNDLRSKIDVMAEQLSQSELTVRKA
                     RKEAASKVQEAEQVIEGLKANIIVLEATLKAQLSEEEQIVGGTDSSTKELDQERDAAI
                     DLNGHHQPQRNGMEVMAEAFAHLQTQAMSKVVGGESGAAVEEKSRTFQIAGVTPLLTE
                     APGKTVSAEIFVGIIAEFSKFANVIETIASLIVRDHVKTLGESLEKFPPKRLPELLES
                     LSNEISDSKRETAFRERFTVLVGGKESTSLIAPETSSETSH"
     gene            complement(3613..4956)
                     /locus_tag="VF182_24625"
     CDS             complement(3613..4956)
                     /locus_tag="VF182_24625"
                     /inference="COORDINATES: protein motif:HMM:NF015194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HEX6440326.1"
                     /translation="MTPRKRTDAAIISLALVLGLTTLAFIIAKTGRDALFFQGSGGLS
                     QLPLIYINIAGAALPLALLFVKAMRIWGARSARLGILLFAAAVLALGSPFLKPGESTL
                     LLAVFMFIPAIFGIIFASLWLLVSDIFENVEKRDAARAFSKIGAATLAGGMVGGLIAK
                     GLTPYLNAKWMIFFGAMIIAAVAVLVRNIHRRFPSNIVTKQGNQQNKQLGILVPLSNK
                     YTRTLLLIAMTGALAGLLIDFQFYAAATSANMGSKGNANFFANFYILLNLSSLLLQLF
                     LTPRIQDKVGLRGGLMVLPLALIGGAGFATAAATALSLSVLRVTEGGLRSSVHRSIWE
                     QAFIPLEPSQRSSVKLAVDGIAARIAEALGALAILIWLKRVAPDGNIPMPLDTRWITW
                     VLLVTVAVWLLVTQTLRAQIRKEQEESTSAPAIEIDCERFPDQCPCTTELGKGIA"
     gene            complement(5808..8456)
                     /locus_tag="VF182_24630"
     CDS             complement(5808..8456)
                     /locus_tag="VF182_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011319522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEX6440327.1"
                     /translation="MTQEEERLEEDRRRTGNWKRWGPYLSDRQWGTVREDYSPNGDAW
                     EYLPHDHARSRAYRWGEDGIGGISDRHQYICFALALWNGKDPILKERLFGLTGNEGNH
                     GEDVKEYYFHLDSTPTHSYMKFLYKYPQREFPYARLVEENRRRSKLEMEFELIDTGVF
                     DDDRYFDVFIEYAKAAPDDILIRIEAINRGPEAAPLHLLPTLWFRNTWAWALDPRRPR
                     LRRDAGAEIAAITLNHYYYGQRWLYCENDPELLFVENETNHTRLFNAENAARFVKDGI
                     NDYLIHKALNSVNPESSGTKAAAHYSMLLQPGASLTARLRFSNLLPTGSKTLDGKFDR
                     LVAQRRDEADEFYRSIIPQNTSQDRRNIMRQALAGLLWNKQFYYYDVDRWLKGDPSGP
                     DPARERLKGRNSEWRHLYNADVISMPDKWEYPWYAAWDLAFHCLALALVDADFAKQQL
                     LLMTREWYMHPNGQLPAYEWAFSNVNPPVHAWAAWRVYKLEKKRRGVADRRFLERMFH
                     KLLLNFTWWVNRKDAAGRNVFQGGFLGLDNIGVFDRSAALPTGGHIEQSDATSWMGMY
                     CLNMLAIALELARKNPVYEDVASKFFEHFVYICDAMNNVGGEQVELWDNKDGFYYDVL
                     QLPDGRRLYMTVRSMVGLIPLFAVETLDPSVVNTLPGFKRRMQWFIENRPALSEHIEI
                     ETTDEGPRRFLSLVNRHRLKRVLGYMLDENEFFSPYGIRALSRYHRDHPYLLAVNGTE
                     HRVDYEPAESSTGLFGGNSNWRGPVWFPVNFLLIESLQKFHFFLGDSYKVECPTGSGQ
                     TANLWQVAGDISQRLTGIFTRDQNGRRPVFGGTEKFQNDPYWRDLIPFNEYFHGDNGA
                     GIGASHQTGWTALVAKLIQQYGEQAD"
     gene            complement(8829..9365)
                     /locus_tag="VF182_24635"
     CDS             complement(8829..9365)
                     /locus_tag="VF182_24635"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6440328.1"
                     /translation="MAEKDHLILTAVGPDQVGLVEKLSQFISGHGCNIEDSKMAVFCG
                     EFAVILLITGEPGSLFKIANDYRTLEAQTALTISIKTPAKRKTAESYLPYRLTASCMD
                     HPGIVYQLSGVLSALGINIEAMETKTYAAPDSGTPIFRLEASISVPAKVNINALRERF
                     FEIQRQENIDLELSPMHH"
     gene            complement(9383..10675)
                     /locus_tag="VF182_24640"
     CDS             complement(9383..10675)
                     /locus_tag="VF182_24640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440329.1"
                     /translation="MSSQSAWEPKPVVAREEVLKLHHEVAAMPDFSINVREDIFRIDA
                     LEMAWDIGVVVYEPSDLTRFPSGPDGNRIGVFLLHGGVSDYKSVDGIARILAQKFGIK
                     VASMTFPGRFYLLDPSRDWPGDVERSDGSARTPLWSKETNITRDQYEIVQDASKRESY
                     GTLISLSAKQGTEFYHRMAAWPVAFEEAIKETARRHLPQGQFSIYIHGHSTGGPFAMM
                     ASQRIANIAGVIGYGTSPFGYMYPQLTGDNWEFPFNQLRLRTWRDTARYLYEGMKDKG
                     AGLPMLIELTFERWEKSKKRPNFKAEDFVHKNSVKSLEAAARAAATRLKMSAKDSEGL
                     VQRYRSYTRELSGPEVKPVPPFLSLHGINDDTVTLSRCDKSRSLFAKLGPAPKVRCIS
                     IGAGVHTWSHTQYDLPQGVATPVAKLWHDAIMNGFFST"
     gene            complement(10958..12814)
                     /locus_tag="VF182_24645"
     CDS             complement(10958..12814)
                     /locus_tag="VF182_24645"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6440330.1"
                     /translation="MAVAIPSAAHGQAAKAIVLAWDGTVSTFVDELARQGKLPNLAKL
                     IAGGAFADDVRPGFPSKTAPGFASLMTGAPPRITGISGNRVPREPRADYTILDSIAGF
                     SAAPLLAEPIWAAAQRAGKKVVISHIPSFAAENSEGTVRLYGYDTITGRDGIVTPRLS
                     KPEPATSWDKLPASDAPVLEISFGVAASKFFGLLVDDPADEQIGYDTLLVTRSRNGED
                     IEVKLKSQPAGSTGELFWSGPVEVKTTGERGATVYFRLFELRGDGSDFFLYFTRPARA
                     MVTPDEALEGAGETVRSFIGNGASILYQQGSFGRTIPNGGSGGAETRYLETVRFAQHQ
                     LMETNRWALAHLPWDLFFAYTPLPDESEHLWRGYLDPGLTTYRKEVADRLRPFLEQVY
                     KTSDDHLGLFLSNRPADTIVAVISDHGMQAADKRFAINRLLQQKGLIIIDEQGRIDLS
                     RTRVLYPSINNGYLLINSTDRKNGIVAPEEREELARQVKELLMDVVDGEQRVIQNVYD
                     AEIRGAEMGIGGPSGGDLYIELAPGYDFDPRTTPAPLITQVEPFGSHGANPEQSSMRT
                     IMLFHGPGIAVGQRLQGVRILDFAPTLAALLDFPAPKNSGGRVLQDVSAALH"
     gene            13062..15092
                     /locus_tag="VF182_24650"
     CDS             13062..15092
                     /locus_tag="VF182_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX6440331.1"
                     /translation="MAKKIPAELEKHGHLRVDNYYWLKDRDNPDVIAYLKAENEHSDR
                     IMGHVKPLEEKIFEEIRSRIAQTDMSVPYKRDGYFYYTRYEQGKEYPIFARRKGSLDQ
                     PEEIMLDANLLAQGHDFFSIGGWALSADQNLMAYAVDTQGRRIYTIYIKNLVTGELLP
                     DVMLDVTENLTWADDNHTLFYAKQDLKTLRAFQIYRHTLGTSAETDQLVFEESDETYV
                     AYVYKTKSKKYLMIVSAQTLSQEYRYLEADDPFGEFKIFLPRQREHEYSIDHYADRFV
                     IRTNDQAKNFRLMVTPVEQIGKEHWQEIVPNRQNVYLGDCEIFRDYLVLEERNRGLTK
                     IRVIPWSGGQGYYLPFDEPAYRVNVGANPEFDTVVLRYEYTSMKTPLSIYDYDMVTHE
                     RTLLKQEQVLGGFDASNYISERLHATAPDGIEVPISLVYRKGLERDGENPLLLYGYGS
                     YGASIDPAFSSPRLSLIDRGFVFAIAHIRGGQELERQWYDDGRVLKKKNTFTDFIACA
                     EYLVREKFTRPEKLFAMGRSAGGLLMGAVANMRPDLFKGIVAEVPFVDVITTMLDPSI
                     PLTTGEYDEWGDPNQEDVYRYMLSYSPYDNVEAKQYPNLLATGSLHDSQVQYWEPAKW
                     VAKLRDVKTDNNRVLLKTNMEAGHGGSSGRFRRHRETAFSYAFLLDLAGIKE"
     gene            complement(15235..16407)
                     /gene="argJ"
                     /locus_tag="VF182_24655"
     CDS             complement(15235..16407)
                     /gene="argJ"
                     /locus_tag="VF182_24655"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX6440332.1"
                     /translation="MQPVAPEQSNHPQGFLSLAKNVGIKDHTLDLTVIYSTVRARAAA
                     MFTRNRFPGAPVIVGRKHVASGFVQALVINSKNANVAMGKQGVANAIETCRIAGKELG
                     ISPYDVLPFSTGVIGRPLPMDKIRAGLKGMRQELKPDNLKLAAEAIMTTDMYPKYIFC
                     QVGSAVIAGIAKGAGMIEPNMATMLVYLMTDAELPSSTMKPMLRRIVERTFNCMSIDT
                     DTSTSDTVVFMANGLAGKVNLRHFEDALSHVCEFLTREIARNGEGATKLIIVDVTGSK
                     SGAQAKSVAKSIVNSPLVKTAVYGCDPNWGRVIMAVGKTFDPAIDPAKVTIGFGHTKV
                     FKKGSPVDCNLQGLRDYLGQSEVTISVDLGIGKTSARVWGCDLTEGYIKENAYYTT"
     gene            complement(16478..17527)
                     /locus_tag="VF182_24660"
     CDS             complement(16478..17527)
                     /locus_tag="VF182_24660"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6440333.1"
                     /translation="MKRRSVMITISVLACLLYFAGPRSGAAQEPYYKGKQIRIIVGLS
                     SGGGYDRAARMLARFMGKYIPGNPDVVVQNMPGAGSVTAANYVWGVAKPDGLTLLAPH
                     NNVYLSQLSGQKEVQFDLAKFQWIGSLENDDMMLFGRADAPYKSIGDIIKAKEQPKCG
                     STGVGSSDYVMSKVLEDTIGAKVTHVTGYPGSSEIAIALERGEVSCMGLTIATYFGRE
                     PFGTWYKEKYVRFLAQSGRKRDSRIAEAPTVYELMDEYKTPATKRRVAEAMLQGGEWA
                     RPLMAPPATPADRVAILRAAHEKASKDPELLAEAKKLRIEITQVRGEQLQSVAKEVMT
                     QPPEVIEQIKKLFVQ"
     gene            17862..19043
                     /locus_tag="VF182_24665"
     CDS             17862..19043
                     /locus_tag="VF182_24665"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6440334.1"
                     /translation="MIIDCDSHLMPRDAFDSVGGRFGSRKPILKFADNGLYADVEFPG
                     NAPQVPGTSPLGAPGSGAMFRSLWDLEARMSDYDERLGIEKHFVLPQFSGWWSYVIEP
                     ELAGRMARSHNESMLRIMEKYPGRILGVALVALQDVAGSICELEWAKERGFATAVVDK
                     IFPVNDHPYGEPLGSRRELWPFFRKAEELNMPLYLHNVQHGHRLSNLLPFQRDGLYVF
                     APQEGHMSLVSLFTSGLLDACPRLQFIYTEAGTAFIKPLVQRLDKILEAPPVDYDDQE
                     EAPLGNGNLSKTGEKLRRARAILPASEFLEKNKQPASHYFKNNFSFTIETEEPELAEA
                     IRFLGAERFLFATDYPHDDPGGRMKFQDVELLRNHPEISEEDKELIRWKNAVRLFHLE
                     S"
     gene            19278..19766
                     /locus_tag="VF182_24670"
     CDS             19278..19766
                     /locus_tag="VF182_24670"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX6440335.1"
                     /translation="MPIPERYNLGYNIGVGGAVVRNGELLLVRRASRHGRGNWQLPGG
                     FIEPDETIEQAVVREVQEEAGVTAEVEAVLGLRSRYDPGSGNGVYIILLLRPVSGEPT
                     ADNHEVDHAGYFTLEQIHGLNPLPPVNWEIAQRVLAPDRRLLYPKPLANLNGAQYTLF
                     VG"
     gene            complement(19963..20337)
                     /locus_tag="VF182_24675"
     CDS             complement(19963..20337)
                     /locus_tag="VF182_24675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440336.1"
                     /translation="MVIRISSAVLSLAGLLALISGLLFWTGSALHLISLHMLLGFLAV
                     GALWVIGVAQAFAPGGSWSIAACALILGALMIAIGMRQSSLLIGNLHWTIQVIHLVLG
                     ILTIGIGHMAAARYRKASVRNS"
     gene            complement(20501..21166)
                     /locus_tag="VF182_24680"
     CDS             complement(20501..21166)
                     /locus_tag="VF182_24680"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX6440337.1"
                     /translation="MTLRRFKDLLFDATRDSFKPLRTMPIIMAAAIMFAGPAAAQNSE
                     NDALKRQPFFSARLSGFNEVIFVAGQAGPPLVAPALRGAISTPAEGRFTAILDGPADV
                     IQYELSYEGLVAPVTQAHIHFGQRHTVGGIVVWLCQTSGTQAPESVRSLTPLCPSQGT
                     VTGTITPAQVLAQDAQGFKAGDFDELVRAIRNQAAYANVHSETFPSGEIRGHINDGRR
                     RNN"
     gene            21489..22577
                     /locus_tag="VF182_24685"
     CDS             21489..22577
                     /locus_tag="VF182_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6440338.1"
                     /translation="MLLINNHEVEKLFDMKACLAALEDAYDDLLIGDAVYRPRLDLWM
                     PCDRPDGYWRWGTMEGASRKIGVFAIRMKSDVVYWPNGDTEEKYCMQPGTWCGLVMVF
                     SVRNGEPLAIINDGLMQHMRVGGCAGLGVKYLAREDASDVGIFGSGGMARTYLEAFYE
                     VRKLKTVRVYSPTKANRESFAREMTGKLDLEVVAVDSPEKLVRGADIIATCTDSTRTV
                     FDEPEWLKPGAHITCVRACEVGPKVVKKCDLSVKLGKNTIEAMDEGMIRLHGNVGYIA
                     GQPEERSRIPNPTVDNYKGDYFKYFMDVRAGKVPGRISDQQLTFFINAGTQGLQFAAC
                     AGKVVQLAKQKKVGRELPTEWFLQDIRD"
     gene            22801..>24376
                     /locus_tag="VF182_24690"
     CDS             22801..>24376
                     /locus_tag="VF182_24690"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEX6440339.1"
                     /translation="MQTGEFFNLYNHGLIRVAVGIPEVQVADPAFNGSQTIELMKRAE
                     HDHAILALFPELGLSAYSCDDLFHQQALLDGSLGSLRSILKVSEALNLIAVVGMPLQV
                     DHLLFNCAVVLSRGRILGIVPKTFLPNYREYYEYRQFAPAAAACSTEVEIFDQPGIPF
                     GAHLLFQAERQREFTFCVEICEDLWVPIPPSSYAALAGATIILNLSASNITIAKDEYR
                     HNLVANQSARCIAAYLYTAAGTGESTTDLAWDGHAIVYDSGVLVSESERFRYDSQLVL
                     AELDLERLALERMRQNTFGQSMQLHREELRRFRKVQFDLELPKTGRLLCSRRIDRFPY
                     VPSDPAVRDRRCYEAYNIQVQGLAKRLRSSNISKIAMGISGGLDSTHALVVAARAMDV
                     LKLPRSNILAYTMPGFATSQHTLENANALMAAIGCESHELDIRPSCEQMLRDIGHPYA
                     AGQPVFDVTFENVQAGERTSHLFRLANLHRALVLGTSDLSELALGWCTYGVGDHMSHY
                     AINASVPKTLIQFLIRW"
BASE COUNT         5327 a         6570 c         6833 g         5646 t
ORIGIN      
        1 atcaagcgtc gtcaactctg cttgtcaaca tgagtgagaa cggctgagaa gccgttccgt
       61 ccgttcaaaa cattccaacg ttcaagacac ggaagcggcg cagccgccgc aacccgcacc
      121 atttgcacat ctttctgact ctaataaatg acgtcctttc cttgaatgta tttacgcatc
      181 ttcgtgcctg ggataaaagc cgagctttct ccgccgtgtt ttcccaagta ctctgagcct
      241 ccggagtgac gaggaatgcg acaaagagct gtccgacatt gggattaacc gaacctttaa
      301 ggacaccggc atgataagca gggcagacct cttaaatact ccttcaggca atcgtaaggc
      361 ttgacctaaa caatagagct gtgccaatgg aaacttgatt tcgctcgccg gtacactcca
      421 accacatcca tgcgccgcga tccaagccga cgaacatctc cagcgctgtc tgatacgtcg
      481 acctcactgt ggtgcgtgcc ggcgtgctga gtcatcctga gaatggaatg cgagcggatt
      541 ggacgaaacc aaaagccgca aaaggttaga ctattatccg atgtagtgag cttgagcgaa
      601 ttgtgagtgg gttgtggccg tgagaaattt tttaagagca cctccagagt ggggtgagta
      661 aggctgacga acgctcgata atgcgagacc atcgttggtc agagtccatg cggtggggag
      721 tttagccctt cagttgaaaa gatcaagaag cggcttggtg tcaaagccac gcatcccgat
      781 gtgatagaaa gccactggca gcttgtcgta tgttttgccg ttgcttggat cagcgcggag
      841 agcgagccct ggcggctcgt gcacattacc agcggctaca cgatgttcgg cctggttctc
      901 ttccgcctgg cgtggggcgt tgtcggcacc cggcatgcgc gcttcacatc ttttgtgcgc
      961 ggccgccctt gtcattctgc gctacgcgcg ctcgcttgtt acgctgcggc cgcagcctta
     1021 cgccggccat aatcctctgg gggctctcgc cattatcgtc ttgttggggc tggttctaat
     1081 cacggtcgcc acgggacatt tggtttataa cgacacggcc ggtgaatggg cggaggaaat
     1141 gcacgaagtc gcggccaggc gtcatgctcg cggtggtcat cgtacacatc atttgagtga
     1201 cggcaaccag ctttctgcac cgcgaaaatc ttgtcggcgc gatgctcacc ggacgcaagg
     1261 ctgcccctcg gggagccgca attcgatggt cgttcctatg gttggcggcg gtgatgctca
     1321 tcctcgtgct ggggttttgg tggatgcagt ggcaaagcgc ggctcaaatc gggaacgcag
     1381 cttcgccgct ggtaacgaac gatccatagg tttaaaagaa ggattaaccg cgacgagaat
     1441 cctgctcgcc gaagacgatc aaatcctcgg cagcggctta aaggcgggcc ttggccaagc
     1501 cggctttcag gtggactggg tccgcgacgg tcagggcgcg caaaaacaaa tcgaagacga
     1561 agaatacgat gctgtcgtcc tcgatctcgg cctactgctc aaagacgggc ttgacgtgct
     1621 gcgccaggcg cgcaagcggg ggaaagccct gccggtgttg gtgctaaccg ccctggtatt
     1681 atccttgggc tggacttact tctgccgagc agcaagacct tatccggggg ctcgaacgca
     1741 cgctcgggac cagaagccgg gaagaacgaa acagacgaga agaaagataa tcaggcaaac
     1801 ggcctatcga tcaaatgagg acagcgacaa tgactctgcc aacgtcggca aggagaaaat
     1861 ttttgaatct ccggaggtga accgatgaaa tcgaaatccc agaaccgcta aaaaagagca
     1921 tgaggagctg cccgccgagc tggtccgagg gactgaactc gggtggcagg acggacaggc
     1981 ggcgaaagcg gttgcaaaaa ttcttcacgc gcatttcctg aaagaggaag ggttcgcgct
     2041 tgcgctactc ggtttattgc ctctcccatc gcccggcaaa gttgattcca agatggaaaa
     2101 cgatcttgcg atggtttcgt gaaggggagg tctgttaaac catcatccgg aggtcactgc
     2161 accactgtca tggtgctcaa cgtcgctcag tgtgatgttt cggacgaagt ttccggtgct
     2221 atgagtgaag tagactcttt tcccccgact aacactgtaa atcgctcgcg aaaagccgtt
     2281 tcacgtttac tatcagatat ctcattgctt agagactcaa ggagttctgg aagccgcttc
     2341 ggcggaaact tctccaaaga ctcgcccaac gtctttacat gatcacggac aataagggat
     2401 gctatcgtct cgatgacatt ggcgaactta ctgaactcgg caattatgcc gacaaaaatc
     2461 tcggccgaga cggtcttgcc aggcgcctcc gttagaaggg gcgtgactcc cgcaatctga
     2521 aatgtgcgcg atttctcttc gaccgctgcc cctgactcgc ctccgacaac tttcgacata
     2581 gcttgggtct gaagatgtgc gaaagcttcc gccatgacct ccattccgtt tcgttggggc
     2641 tggtggtggc cgtttagatc gatcgccgcg tcgcgttcct gatccagctc cttcgtggac
     2701 gaatcggtgc cgccaacaat ctgctcttcc tcgctaagtt gagctttcag cgttgcctct
     2761 agcacaatga tgtttgcctt gagaccctca ataacttgct cggcttcttg gaccttactc
     2821 gctgcttctt tgcgtgcctt tcgaaccgtc aattccgact ggcttagctg ctctgccatg
     2881 acatctattt tagaccggag gtcgtttact tccgatgttc gactttgaag cgctgcttct
     2941 ttctccttca atgcatgttg taaattgtcg atctcggtcc taaggctttc ttccgtcttt
     3001 ttgccggcaa tgttctggct atgaaccatg tcctcggtct ctctaaccct agcctctagt
     3061 tcgctgatgt ttgccttgag accctcaata acttcctcgg catgtttggt cttgctcagt
     3121 gctatagctt gattgtcact gacggctgcc tttaacctac ggattccgtc cgcaaatatc
     3181 ttatccagct cctctgttgc atcgcagaga ctgtcttcgt tccctgcagc ctgacgggaa
     3241 accgtcgtat cgggtgtcgt ttgggttaaa gttccttgca cgacttctct gaccaattca
     3301 tagactctgc ccattatgtc cccccggcat tcttcaggcg ttaatttgtg cgcccaatac
     3361 gaaagatgag tatccgctgg tgattaaact aaggatgaag ctcgaatcga atgctgagtc
     3421 ctagggcggg tgtaaacgat tgtcaagaga aaaattcgtt gggaaaaata aattttcaga
     3481 gcgctgcgta ccgttgcaca gatatttgca tcagtgcacg tagaagcaag cgaggatcgc
     3541 cggcgcgagc tacggcccgt cgctcagcag agagtgagga agtgcatttg gcgaagcggc
     3601 aagctagtgc cgttacgcga tgcctttgcc gagctcggta gtgcacgggc attgatcggg
     3661 aaagcgctcg cagtcgatct cgatagccgg cgcggaggtg ctttcctcct gttctttccg
     3721 aatctgcgct ctcagagttt gggtgaccag caaccagacc gcgaccgtta ccagtaacac
     3781 ccaggtgatc cacctggtat cgagtggcat gggaatgttg ccgtcgggag ccacgcgttt
     3841 gagccagatg agaatagcca gcgcgcccag cgcttcggcg atcctcgcgg caatgccatc
     3901 cactgccagc ttcactgacg agcgttggga aggctccagc gggataaaag cctgttccca
     3961 aatcgaccgg tgcaccgagg agcgcagtcc cccttcagtg actcgcagca ccgacaaact
     4021 aagggccgtt gcggcggcgg tcgcgaaacc ggcgccgccg atcagcgcaa gcggcaggac
     4081 catgagcccg ccgcgcaatc cgactttgtc ctggatcctc ggcgtcaaaa aaagttgcaa
     4141 cagcagcgag cttaagttca acaggatata aaaattcgcg aagaagttcg cgtttccttt
     4201 cgatcccata ttggcgctgg ttgcggccgc atagaattgg aagtcgatga gcaagccggc
     4261 cagggctccg gtcatggcaa ttagtagaag agtgcgcgtg tacttgttcg acaacggaac
     4321 cagaatacca agttgcttat tctgctgatt gccttgcttc gtgacgatat tgctgggaaa
     4381 gcggcgatga atgttccgca ccaggacagc cacggcagcg atgatcatcg cgccgaaaaa
     4441 tatcatccat ttcgcattta gatagggagt gagtcctttg gcgatgagtc cacccaccat
     4501 gccgccggcg agtgtcgccg cgccgatctt gctgaaggcc cgcgccgcgt cacgtttttc
     4561 cacattctcg aagatgtccg agaccagtag ccacagactc gcaaagatga tcccgaagat
     4621 tgccgggatg aacatgaaga ccgctaacag cagcgtgctt tcgcccggct ttaaaaaagg
     4681 cgatcccaga gccagtaccg cagcggcaaa tagcagaatt ccaagccggg cagagcgcgc
     4741 gccccagatc ctcatagcct tgacgaacag cagcgccagg ggcaaggcgg cgccggcgat
     4801 gttgatataa atgagcggca gttgtgatag ccccccgctg ccttggaaga acagggcatc
     4861 gcgtccggtt ttcgcgatga taaacgccaa cgtggtcaat ccgagcacaa gcgcaagaga
     4921 gataatggct gcgtcggttc gttttcgagg ggtcataatc ttgctcgccc tagatgatgg
     4981 cgtgccggca gtgttcgatt cgtaactcgg gatcgaccag ccgttgccaa tatgcccaac
     5041 tcaaccgccc aagtcgaacg gtttagatac ttttatgata aagcagatga ttgtgcgctt
     5101 gtgagcgaga tcgacgtact gcgataaacc cggattccgc agttgcgatt gacagaagcg
     5161 aggcacatcc ttccgccgcg aagtcgatgg gtttctgcct gagcagatat cgctcagcag
     5221 tgcttcgagg gggatgttta agactcatag caacgacgtg cgagagcgga aaatcatttt
     5281 gaagcttcac agtcgacgcc ttcgctgctc caggattcca cctcgctttc ggcattttta
     5341 cttcgactgc acaatcctgg ataaataacg agttaatcac ggcgggttgg gtttcccgcg
     5401 cagcttcctg cgtcaagaac tattcccaga tcattagagt ccgtcattcc cgcatgtttt
     5461 tagcgggaat gcaggcgaga ccggaacccg accccgatca agacattcgg ggtggcgcct
     5521 tgggggaacc tctcgccgtc atgttctgac attcgtcagt ttgcttcggt taattcattg
     5581 acggcccaca ccggggatgc ctgcgcttgc tatgcggggt tgagcgctat cgtacgatta
     5641 aactccacta gcagggtgct taaagcctct tgcaggctgc tcaaaaagct tcaggtggga
     5701 gacgcgcgaa ttacaaggga tgaaagcgga aatctgaaag cggaattcag tcttcagctt
     5761 tcataattca tcttttcgaa accatatgaa ttcttttccg cagcctgcta gtcagcctgt
     5821 tcgccatact gttgaatcag ctttgccacc aaagcggtcc agccggtctg atggctggcg
     5881 cctatgcccg cgccgttatc gccgtggaaa tactcgttga agggaatcaa gtcgcgccag
     5941 tatggatcat tttgaaactt ctcggtgccg ccgaagaccg gccgcctgcc attctggtcg
     6001 cgcgtaaaga tcccggtcag tcgttgcgaa atatcgcctg cgacctgcca tagattggct
     6061 gtctgaccgg agccggtggg acattctacc ttgtagctat cgccgagaaa aaaatgaaat
     6121 ttctggagcg attcgataag caggaaattt actggaaacc agactggccc gcgccagttg
     6181 gaattgccgc cgaagagacc ggtgctcgat tccgccggct cgtaatcgac gcggtgctcc
     6241 gtgccgttga cggccagaag gtacggatga tcccgatgat agcgcgagag agcgcggatg
     6301 ccgtatgggg aaaagaactc gttttcatcg agcatgtacc cgagcacgcg cttgagccgg
     6361 tgacgattga ccagcgatag aaaacggcga ggcccttcgt cggtggtttc gatctcgatg
     6421 tgttcgctca aagccggacg attttcgata aaccactgca tgcgtcgctt gaacccgggc
     6481 agcgtgttga caacggaggg gtcgagagtt tccaccgcga acaacgggat cagaccgacc
     6541 attgaccgca ccgtcatata caaccgccgt ccgtcgggga gttgcaggac gtcataataa
     6601 aacccgtcct tattatccca caactcgact tgctcgccgc cgacattgtt catcgcgtca
     6661 caaatgtata cgaagtgctc gaagaacttg ctcgcgacgt cttcataaac gggattcttc
     6721 cgcgccagct ccaaggcgat cgccagcatg ttcaggcagt acatgcccat ccagcttgtt
     6781 gcatcggact gctcgatgtg gccgcctgtc ggtaatgccg cgctgcgatc aaaaacgccg
     6841 atgttgtcga gtccgagaaa gccgccctgg aaaacgtttc tgcctgcagc atcctttcgg
     6901 tttacccacc aggtgaagtt aagcagaagc ttgtgaaaca tgcgttcgag aaatctccgg
     6961 tcggcgacgc cgcgccgctt cttctcgagt ttgtaaacac gccaggccgc ccatgcgtgc
     7021 accggcggat tgacgttgct aaaggcccat tcgtaggcgg gtaactggcc gttgggatgc
     7081 atgtaccatt cccgggtcat cagcagcagt tgctgcttgg cgaaatccgc atcgaccaac
     7141 gcaagcgcaa ggcaatgaaa cgcgagatcc caggcggcat accacgggta ttcccatttg
     7201 tccggcatcg agatcacgtc ggcattgtaa agatgccgcc attccgaatt ccggcctttg
     7261 agacgttcgc gggcaggatc gggtccggac gggtcgcctt tgagccagcg gtcgacgtcg
     7321 tagtaataaa actgcttgtt ccacagcaga ccggctaatg cctgacgcat aatattgcgg
     7381 cggtcctggc tggtgttctg tggaatgatc gatcgataaa actcgtccgc ttcgtcgcgg
     7441 cgttgcgcca caagccgatc gaatttgcca tcgagcgtct tgctccctgt cggcaaaaga
     7501 ttgctgaaac ggagccgagc cgtgaggctt gctccgggct gtaacagcat tgaataatgc
     7561 gctgccgcct tggttcccga agactcggga ttcacactgt tcaaagcttt atgaatcaag
     7621 tagtcgttga tgccgtcttt cacaaaacga gcggcattct cggcgttgaa caaacgggtg
     7681 tgattggttt cattctcgac gaacagcaac tcgggatcgt tctcgcagta aagccagcgt
     7741 tggccgtagt agtagtgatt gagcgtaata gccgctattt ccgcgcccgc atcgcggcgc
     7801 aggcgcggcc tgcgcggatc gagggcccac gcccaggtgt tgcgaaacca cagggtggga
     7861 agtagatgga gcggcgcagc ctcgggtccg cggttgatcg cctcgatgcg aatcaaaata
     7921 tcatcaggcg ccgccttggc gtattcgatg aatacgtcga agtagcgatc gtcgtcgaaa
     7981 actccggtat cgatcagctc gaattccatc tctagctttg atcgcctgcg gttctcctcc
     8041 acgagtcgcg catagggaaa ttcgcgctga gggtacttgt aaagaaactt catgtacgag
     8101 tgggtgggtg tggaatcgag atgaaaatag tattctttaa cgtcttcgcc gtgattgcct
     8161 tcattacccg tgagcccgaa gagccgttcc ttgaggatcg gatctttgcc attccacaga
     8221 gccaaagcaa agcagatata ctggtggcga tcggagatgc caccgatgcc gtcttcgccc
     8281 catcggtagg cgcggctgcg cgcgtgatcg tgaggcaggt attcccaggc gtcgccgttc
     8341 gggctgtagt cctcgcgcac cgtgccccac tggcggtccg ataaataagg tccccaacgt
     8401 ttccagtttc ccgtgcgccg gcgatcttcc tctaatcttt cttcttcctg ggtcacgtta
     8461 cgttcccgag cgggttgccg aataacgttg cagtctgagc tcccagtgca cgtcaaacgc
     8521 gatgcctgac tcggtgcttt ctattgtatt gtaccggcta ctttttccat tcgttagatg
     8581 ttacttcgaa gccgttgggc ggcagtggct ccttgccatt cactgctctt cagccgcgct
     8641 gtagcggcca actgccaata ccccgggagc cgggcctgtc caaaggtgct tatagcctat
     8701 agcaggtgag acagggtacc agggctattc cggatgcttt gatttgaggc gtttgctatt
     8761 gcattgacca ggcttgggtg ctgcggctgc cgcattagct ctgaatcggt aattcagctg
     8821 atccttggct agtgatgcat cggcgagagt tcgagatcga tattttcttg ccgctgtatc
     8881 tcgaagaaac gttcacggag cgcgtttata ttcaccttgg ccggcaccga gatgctggct
     8941 tccaatcgga agatcggcgt gccgctgtcc ggggctgcat acgtcttggt ttccatggcc
     9001 tcgatgttga tgccaagagc gctcaaaacg ccgctcaact gatagacgat gccgggatgg
     9061 tccatgcacg acgccgtcag gcggtagggg agataggatt ccgcagtctt gcgtttggcg
     9121 ggtgtcttga tcgaaatcgt tagagcggtc tgcgcttcta gggtgcggta gtcattggca
     9181 atcttgaaca ggctgccggg ctctcccgtg atcaagagga ttacggcaaa ttcgccgcag
     9241 aagaccgcca tcttgctatc ttcgatgttg cagccgtggc ccgaaataaa ttgcgaaagc
     9301 ttttcgacca gacccacctg atcgggtccc accgcggtca gaataagatg atctttttca
     9361 gccataaata ctcttcattc agttacgtcg agaaaaaacc attcatgatg gcatcatgcc
     9421 agagtttggc caccggtgtg gcgactcctt ggggaagatc gtactgggta tgcgaccagg
     9481 tatgtactcc cgcaccgatg gatatgcagc gaactttggg agcgggaccg agcttggcaa
     9541 aaagcgaccg cgacttgtca cagcgggaca aagtgacggt atcatcgtta ataccgtgca
     9601 acgagaggaa aggcggcacc ggtttgactt cgggaccgga taattcccgg gtgtagctcc
     9661 gataccgttg cacgaggccc tcagaatctt tagccgacat tttcaaccgc gttgccgcag
     9721 cccgagcggc ggcctcgagc gatttaactg aattcttgtg cacgaaatct tcagctttaa
     9781 agttcggccg ttttttgctt ttctcccaac gctcgaatgt gagctcgatc agcatcggca
     9841 agccggcgcc tttgtctttc atcccttcgt agaggtaccg cgcggtatcg cgccatgtgc
     9901 gaagccgcag ctgattgaac gggaactccc agttatcgcc ggtgagctgc gggtacatgt
     9961 aaccgaatgg agaagtgccg taaccgatga cccctgcgat attggcgatt cgttggcttg
    10021 ccatcatggc gaacggcccg ccggtggagt ggccgtgaat atagatcgag aactggcctt
    10081 gcgggagatg gcgccgtgcg gtttctttga tggcttcctc gaaggccacc ggccaagccg
    10141 ccatacggtg gtaaaactca gtgccttgct tggccgagag cgaaatcaaa gtcccgtagc
    10201 tttcgcgctt ggaagcatct tgcacgatct cgtactgatc cctggttatg ttggtttctt
    10261 tactccacag cggcgtgcgc gccgagccat cggatcgctc aacgtcgccg ggccagtccc
    10321 tgcttggatc gagcaagtag aacctgccgg gaaaagtcat gctggcgact ttgataccaa
    10381 atttctgtgc caggattcga gcgatgccat cgacggactt ataatccgag acgccgccgt
    10441 gaaggaggaa gacgccgata cgattgccat cgggtccgga tggaaatcgc gtgagatcgg
    10501 agggctcgta aaccaccacg ccgatatccc aggccatttc caaggcatcg atgcggaaaa
    10561 tgtcttctcg gacgtttatc gagaagtccg gcatggccgc gacctcgtga tgaagcttga
    10621 ggacttcttc acgggcgacg accggtttcg gttcccaggc agattggctc gacattgtcg
    10681 aatgttcttt gaggcaccgg cgagcttgac gaatttgacc ctattcgaca ttactctaag
    10741 cccgcgacgc cagtgagccc tacagccgtg ggcagtggga ccgtccatcg gaggccgccc
    10801 cgcattcccc tgagggtgat gttgcgtacg tcgaggcctt atagccaact gcgcgttcgg
    10861 gtgcccacgg cttccggtct cgccgtcttt gctcaattct gacggtgcca agtgttgtcg
    10921 gattaggctt cagactatgc agaagccaac agctgtgtca atgcagagcg gcggaaacat
    10981 cctgcagcac gcggccgcct gagttctttg gagcaggaaa atcgagcaat gcggcaagcg
    11041 tcggagcgaa atcgagaatg cggacacctt gcaaccgttg cccgacggcg attcccgggc
    11101 catggaagag catgatagtg cgcattgaac tttgctccgg gttggctcca tggctaccga
    11161 acggttcgac ttgtgtgatc agcggcgccg gagtcgttcg tggatcaaaa tcgtagccgg
    11221 gggcaagctc gatatagagg tcaccacccg aaggaccgcc gatgcccatt tctgcgccgc
    11281 ggatttcagc gtcataaacg ttttggatta ctctttgttc accatcgaca acatccatga
    11341 gcagttcttt tacctggcgc gcgagctctt cacgctcttc gggagcgacg ataccgttct
    11401 tgcgatccgt cgaattgatc agcagatagc cattattgat cgacggatag agaaccctcg
    11461 ttctagacag atcgatgcga ccttgttcgt cgatgatgat cagccctttc tgttgtaaca
    11521 gccgattgat cgcaaaacgc ttgtcagctg cttgcatgcc gtggtcggaa ataaccgcca
    11581 cgatcgtatc ggccgggcga ttggataaga aaaggcctaa atgatcatcc gaggttttat
    11641 aaacctgctc caagaacggt cgcaggcgat cggcgacttc ttttcggtaa gtcgtgagcc
    11701 cggggtccag atagccgcgc caaagatgct cgctctcgtc cggaagtggg gtgtaggcaa
    11761 agaacaggtc ccagggaaga tgcgccaatg cccagcggtt ggtctccata agctgatgct
    11821 gcgcaaacct gaccgtttcg aggtatcttg tttcagcgcc gccgctgccg ccgttgggga
    11881 tggttcgacc gaagcttccc tgctgataga gaatgcttgc gccgttgccg atgaacgaac
    11941 gaaccgtctc tccggcgccc tccagcgcct cgtcgggagt gaccatggcg cgggcgggcc
    12001 gggtgaagta aagaaaaaaa tcgctgccat cgcctctcag ctcgaacagt ctgaaataaa
    12061 ccgtggcgcc ccgttcgccg gtggttttga cttctaccgg gccgctccag aagagttccc
    12121 ctgtgctgcc ggcaggctgg ctcttgagtt tcacttcaat gtcttcgccg ttgcggctgc
    12181 gggtcaccag cagggtgtcg tagccaatct gttcatccgc cggatcatcg acgagcagac
    12241 cgaaaaattt ggaagcggcg acgccaaaac tgatttccag caccggcgca tcgctggccg
    12301 gcagcttgtc ccaagaagtc gccggctcgg gtttgctcag gcgcggtgtg acgatgccat
    12361 cccgtccggt aattgtgtcg tagccgtaga gtcgtacggt accctccgaa ttttctgctg
    12421 caaaggaggg aatatgggag atcaccacct ttttgccggc gcgttgggca gcggcccaga
    12481 tcggctccgc gagcagaggc gccgccgaaa acccggcaat gctgtcaaga atcgtatagt
    12541 cggcccgggg ctcccgcggc actcgattgc cgctaatacc ggtaatgcgc ggtggcgcgc
    12601 cggtcatcag cgaggcaaaa ccgggcgccg tttttgaggg aaaaccgggc ctcacatcgt
    12661 cggcaaaagc accgccggca atgagcttgg ccaggttcgg cagctttcct tgccgcgcca
    12721 gctcatcgac aaaggttgaa accgtgccgt cccacgccag cacgatggct ttagctgcct
    12781 ggccgtgagc cgcgctggga atggcgacgg ccacgatcgc acaggctaaa agtgcgattt
    12841 tacgaagaga aacaaccatc gggatgacga gagtagccga tctgaaaaca agctgtcaat
    12901 gtgatgcgtc gagtcagctt gtcaaaagag cctgaaggcc gtttgcggct acgctgattt
    12961 tttcataatc aatgctggtg agtctcaggc gaaccgaatg cggagtgtct tgaggttgtt
    13021 cgattttgta ctaggttcgc aggaaatcgg aaaggcaatc aatggcgaaa aaaatccccg
    13081 ccgagcttga aaagcacggc cacttgcgtg tcgacaacta ctattggcta aaagatcggg
    13141 ataatcccga tgtgatcgct tacctgaaag ctgagaacga gcactccgat cggatcatgg
    13201 gtcatgtcaa gcctttggaa gagaagatct tcgaggaaat cagatcccgt attgcccaaa
    13261 ccgacatgtc ggtcccctac aaacgtgacg gttatttcta ttacacccgc tacgaacagg
    13321 ggaaagagta tccgattttc gcgcgcagga aaggttcgtt ggatcaacct gaagagatca
    13381 tgctcgatgc caacctgctg gcgcagggcc atgatttttt ttccatcggc ggctgggccc
    13441 tcagtgccga tcaaaatctg atggcctatg cggtggatac gcaagggaga aggatttata
    13501 cgatttacat caagaatctt gtcaccgggg aattgttgcc tgacgtcatg ctcgacgtta
    13561 cggaaaatct cacctgggcc gacgataacc acacgctttt ttatgccaag caggacctta
    13621 agacgttgcg cgcgtttcag atctaccgcc acacgcttgg cacctccgcc gagaccgatc
    13681 agctggtgtt cgaggaatcc gatgaaacct atgtggcgta tgtctacaag accaaatcga
    13741 aaaagtatct catgattgtc tcggcacaaa ccctcagcca ggaatatcgt tacctcgagg
    13801 ctgacgaccc gttcggtgaa ttcaaaatct ttttaccgcg gcagcgtgaa cacgaatatt
    13861 ccatcgatca ctacgccgat cgcttcgtga tacgcaccaa tgatcaggcg aagaattttc
    13921 gtctcatggt cacgccggtg gaacaaatcg gcaaagaaca ctggcaggag atcgttccaa
    13981 atcggcaaaa tgtctatctc ggcgactgcg agatctttag ggactatctg gttttggagg
    14041 agcgtaaccg gggtctgacc aagatccgcg tgataccctg gtccggcgga cagggctatt
    14101 acctcccgtt cgatgagccg gcgtatcgcg tcaatgtcgg agctaatccg gagtttgata
    14161 ccgtcgtatt gcgctatgaa tatacttcga tgaagacgcc gttatcgatt tatgattacg
    14221 acatggtgac gcatgaacgg acgctgctga aacaagaaca agtgttgggc ggttttgatg
    14281 caagtaacta tatttccgag cggctgcatg caaccgcgcc ggatgggatc gaagtcccga
    14341 tctctctggt ctatcgcaag ggattggagc gagatggtga gaatccgttg cttctctacg
    14401 gttacggctc ctacggcgcc agcatcgatc cggcattttc ttcgccgcgc ttgagcctga
    14461 tcgatcgtgg atttgttttt gccatcgctc atatccgtgg cggtcaagag ctggagcggc
    14521 agtggtatga tgacggcaga gttttgaaga agaaaaatac ttttaccgac tttattgctt
    14581 gcgccgaata tctcgtgcgc gagaaattca cccgtccgga aaagctgttt gccatgggtc
    14641 gcagcgcggg tggcctgctg atgggcgccg tcgccaatat gcggccggat ctgttcaagg
    14701 gaattgttgc cgaagtaccg tttgtcgatg tgatcacaac gatgctcgat cctagtatcc
    14761 cgctgacgac gggagaatac gacgaatggg gtgatcccaa tcaagaagat gtttatcgat
    14821 acatgctctc ctattccccc tacgacaacg tggaggccaa gcagtatccc aatctcctag
    14881 ctaccggcag cttgcacgat tcccaagtgc agtactggga gccggcgaag tgggtcgcca
    14941 agctgcggga tgtaaagacc gacaataacc gggtgctgct aaaaacgaat atggaggccg
    15001 gtcacggcgg ctctagtggc agattccggc gtcatcgcga gactgcgttt tcctatgcct
    15061 ttctcctcga tctggctggg atcaaggaat aatcgttttg gctcgaccgg gaggattccg
    15121 aagcgtcaga tctgtcaaag aaaccgaggc gggcgctact gcgctggtct ggttggatcg
    15181 ttgaaggtca cttgcctaac gatcgtttta tcacgccacg aattgtaacg acattcaggt
    15241 cgtgtaatag gcattttctt tgatgtatcc ttcggtcaga tcgcaacccc atacgcgcgc
    15301 tgaagttttg ccaatgccaa gatcgaccga aatcgtgact tcggattggc caagatagtc
    15361 gcgcaatcct tggagattac aatcgacggg agagcctttc ttgaacacct tggtgtggcc
    15421 gaaaccgatg gtgaccttgg caggatcgat ggcaggatca aaagttttgc ccaccgccat
    15481 gatcacacgg ccccaattgg gatcgcatcc atagacagcc gttttcacca atggcgagtt
    15541 cacaatcgac ttcgctaccg attttgcttg agcgccgctc ttcgaacccg tgacatcgac
    15601 aatgatgagt ttggtggccc cttcaccgtt ccgggcgatc tctcgggtga gaaattcgca
    15661 aacatgcgat agcgcgtctt cgaaatgccg cagattgact ttgcccgcca acccatttgc
    15721 catgaatacg acggtatcgc tggtgctggt atcggtatcg atgctcatgc aattgaaagt
    15781 tcgttcgaca atgcggcgca gcatgggctt catcgtcgag ctcggtagtt ccgcatcggt
    15841 catcagatag accagcatcg ttgccatatt cggctcgatc atgccggcgc ctttggcgat
    15901 accggcgatg actgccgaac ccacctggca gaaaatgtac ttcggataca tgtcggtagt
    15961 catgatggct tcggccgcca atttgagatt gtcaggcttt agttcttggc gcatgccttt
    16021 taatccggcg cgaattttgt ccatgggcag cggccggcca atgacgccgg tagaaaaagg
    16081 cagcacgtcg tagggactga tgccgagctc tttgccggca atccggcagg tttcgatcgc
    16141 attggcgact ccctgctttc ccatggcgac gttggcgttc ttgctgttga tcaccagcgc
    16201 ctggacgaag ccactggcga catgcttcct gccgacaatc accggcgcgc cagggaagcg
    16261 attgcgggta aacatcgctg cggcgcgagc gcgcaccgtg gaatagataa ccgtgagatc
    16321 cagcgtgtgg tctttgatgc cgacattttt tgccaaagag agaaatcctt ggggatgatt
    16381 agattgttcg ggcgcgaccg gttgcatgtg tcggcgattt tctcctgaag tttattggtt
    16441 gatggatcga gcgcagcgac aagcgcgccg cttgttctta ctgcacgaac agcttcttta
    16501 tttgttcgat cacctctggc ggttgggtca tgacttcttt cgctaccgat tgtagctgtt
    16561 cgccgcgcac ctgcgtgatc tcaatgcgca gtttcttcgc ctccgcgagc agctccggat
    16621 ctttgctggc cttttcatga gcggcgcgca gaatggcgac acgatccgcg ggcgttgccg
    16681 gcggcgccat caacgggcgc gcccactcgc ctccctgaag catcgcctcg gctacacggc
    16741 gtttggttgc cggtgtttta tattcatcca tcagttcgta gacggtcggc gcctcggcga
    16801 ttcggctgtc gcgtttgcgg ccgctctggg caaggaacct gacatacttc tccttgtacc
    16861 aggtgccaaa cggctcccgg ccgaaatagg tggcgatggt caatcccata caggaaactt
    16921 cgcctctttc cagggctatc gcaatttcgc tgctgccggg gtagccggta acatgagtca
    16981 ccttggcgcc gatggtgtcc tccagtacct tggacatcac atagtccgaa ctgcccaccc
    17041 cggtggagcc gcacttgggt tgttcttttg ctttgatgat gtcgcctatc gatttataag
    17101 gcgcgtcggc gcgaccgaac agcatcatat catcgttttc gagagagccg atccattgga
    17161 atttagccaa gtcgaattgg acttcttttt gtcccgagag ctggctcaag taaacgttgt
    17221 tgtgcggcgc cagcagggtc aggccgtccg gctttgccac tccccagacg taattcgccg
    17281 cagtgacgga gcccgcgccc ggcatatttt gaacgactac gtcggggttt cccggaatat
    17341 acttgcccat gaaacgtgcg agcatgcgcg ccgcgcggtc gtacccgccg ccgctgctca
    17401 gtccgacgat aatgcgtatt tgtttgccct tgtaatacgg ctcctgcgcc gcacccgacc
    17461 gcggcccggc gaaatataac agacacgcta aaaccgagat tgtaatcatc acactgcgcc
    17521 gcttcatgtg ttttcctcca atggtgtttt aatcactggt tcgaaataat tggatcgtaa
    17581 acttattgat ttgctttgca atacgcaagg aatgctcttg gtggtggcct actaaccgac
    17641 aggcgcgcaa atgtcgatcg gaaatagtat gagagtctcg gtctcatgct cacgttcatc
    17701 gatcatgacc ctgatcagaa ttttcggttt tactcctttt attcagcaat cttttgatgc
    17761 tgttcgcagc tgttggtaat ttgtccttca ttgcgccttc cttgacttca ttatcctgcg
    17821 cgccctataa gaaactagtt ctctagtaat gaggagtaat cgtgataatc gattgcgata
    17881 gtcatctgat gccccgggac gcttttgaca gtgtcggcgg ccgtttcggc tcgcgcaagc
    17941 cgatactaaa atttgccgac aatggcctgt acgccgatgt cgagtttccc ggcaatgcgc
    18001 ctcaagtgcc gggcaccagc ccgctcggcg cgcccggttc cggcgccatg ttcaggagct
    18061 tgtgggatct agaggcgcgc atgagtgact acgatgagcg tctcgggatc gaaaagcact
    18121 tcgtgttgcc gcaattttcc ggctggtggt cctatgtgat cgaacctgaa ctggccggcc
    18181 ggatggcccg gtcgcacaat gaatcgatgt tgcggatcat ggagaagtat cccggcagga
    18241 ttctcggcgt ggcgctggtc gcactgcagg acgttgccgg atcgatctgc gagctggagt
    18301 gggccaagga acgcggcttt gcgaccgctg tcgtcgataa gatttttccc gtgaacgatc
    18361 atccttacgg tgagcctctc ggcagccggc gcgagctctg gccgttcttc cgaaaggccg
    18421 aggagctgaa catgccgctt tacctgcaca atgttcagca tggccatcgc ctcagtaacc
    18481 tgctgccgtt ccagagagat gggctttacg tattcgcgcc gcaggaaggg cacatgagct
    18541 tagtttcatt gtttaccagc ggcttgctgg atgcttgccc cagattgcag ttcatttaca
    18601 ccgaagcagg gaccgcgttt atcaaacctt tggttcagcg tctcgataag attctcgaag
    18661 cgccgccggt ggattatgac gatcaggaag aagcgccgct gggcaacggc aatttgtcca
    18721 aaacaggcga gaagctgcgc cgggcgcggg cgatcttgcc ggcgagcgag ttccttgaaa
    18781 aaaataaaca gccggcgagc cattatttta aaaataactt ttcctttacc atcgagaccg
    18841 aggagccgga gcttgccgaa gccatccgct tcctcggcgc tgaacgcttt ctcttcgcca
    18901 ccgattaccc gcatgacgat ccaggcggaa gaatgaaatt ccaggacgtg gagctgctta
    18961 gaaaccatcc ggagatttca gaagaagata aagagctgat ccgctggaag aacgccgtcc
    19021 gtttgtttca tctggagtcg tgattgaact ctcatggctc agttgcgtcg gcatgctgcg
    19081 cagtgatgtt agtgactcag ctagagtccg tcattcccgc atgcttttag cgggaatcca
    19141 ggcgaatatc agactggacc cccgattaaa acattcgggg gtgacaactg ggggaagtta
    19201 tccaaagtcc tcaagtacgc ggacgcttgc tgcgaggttg ttgttgttca ttacagtcgg
    19261 ttaggatcac gcacgttatg ccaatacccg agcgctacaa tttaggttac aacatcggtg
    19321 tgggcggcgc ggtggtgcgc aatggcgagc tcttgttggt gcggcgcgct tcgagacatg
    19381 gccgcggcaa ctggcaactg ccgggcgggt tcatcgaacc cgatgaaacc atcgaacagg
    19441 cggttgttcg ggaagtacag gaagaggcgg gagtgacggc cgaagtcgaa gcagttttgg
    19501 ggttgcgtag tcgctatgat ccggggtcgg gaaatggcgt ctacatcatt ctcctattga
    19561 gaccggtgag cggcgaaccc accgccgata accacgaagt cgatcacgcc ggctatttca
    19621 cattggagca gattcacggt ttgaatccct tgccgccggt caactgggag atcgcccaac
    19681 gagtgctggc gccggaccgc cgattgctgt atcccaaacc gctggccaat ttaaacggcg
    19741 cgcagtacac gctgttcgtc ggctgaataa cagttctcaa gtctaggcgg gagcctcatc
    19801 tatttgcgag cttctgttct gttctgaaga ctgagatcag caatttgatc ctaataaaga
    19861 tcgagttttt gaaggagaaa tcagttcaat cgcacggtta acgcgatgct cacccgcatc
    19921 ctgagcagcc tgcaacacag tccacctagc catggcgaga ctctaactat tgcgaacgga
    19981 ggcttttcga tagcgcgcgg cggccatgtg gccaatgccg atcgtcaaga tcccgagcac
    20041 caagtggatg acttgaatgg tccagtgaag attaccaatc agaagcgatg attgcctcat
    20101 gccgatggcg atcatgagcg cgccaagaat tagcgcgcaa gctgcgatac tccaactgcc
    20161 acccggagca aatgcctgcg cgactccaat cacccagagc gcgcctacgg ccaagaaccc
    20221 gagaagcatg tgcaatgaaa ttagatgtag agcgctgccg gtccagaaca acaagcccga
    20281 gatcaaggcg agcaatccag ccaatgagag aacggccgat gagatacgaa tcaccattct
    20341 ataagccttt cctttggcac ccctcgtcac taaagattct caacgaggtg acgttgactt
    20401 ccgtccggct tgctgatgtc tgcgacgtca aaaactgaaa agccccatcg gcggggcttt
    20461 tcaagcgggc gggcgctcgg attccatttg ctatcgcagt ctaattgttt ctcctgcggc
    20521 catcgttgat gtgaccacgg atttcaccgc tgggaaacgt ttcagagtgg acgttggcgt
    20581 aagccgcttg attgcggatg gcgcgaacga gctcgtcaaa gtctccggct ttaaatccct
    20641 gcgcatcttg ggcaaggacc tgtgcaggag taatcgtccc ggtcacggta ccttgtgagg
    20701 gacagagtgg cgttaaagaa cggacggact ccggggcctg cgttccgctg gtttggcaca
    20761 gccacacgac gatgccgccg accgtatggc gctgaccgaa atggatgtga gcctgggtca
    20821 ccggcgctac gagcccctcg taactaagct cgtactggat cacgtccgcc ggcccatcga
    20881 ggatagcggt gaaccgcccc tcggcggggg tcgatatcgc accccggagg gcaggtgcta
    20941 ccagcggcgg tcctgcttga ccggcaacaa aaataacctc gttgaacccg gatagcctgg
    21001 ctgaaaaaaa aggttgtctt tttagcgcat cattttcgga gttttgagcc gctgccggtc
    21061 cagcaaacat tatggcggcg gccatgataa tcggcattgt tctcagcggc ttgaaggagt
    21121 cgcgagtcgc gtcgaacaaa agatctttga atcgtcttaa cgtcatcgtc cttccctcct
    21181 caaaacggtt acgagagctt tgtaaatcgc tctcacctat gattttgccg aagccgggcg
    21241 ccgccgaatc tcgccaacat aactaataat ctttaacctt ctcggtgaac ccgcgtcaat
    21301 cctccctaag aattatttgg aacttcaaga agcatgtgga aacatttctc cgggccccat
    21361 tagtttcgtc gtccaacggg actgaagccg aatcgccttt tacaccagag ctcttggtga
    21421 tcgccccatt ccgggttcgt cttttcaaat ctcacggatt catataagca accagcagga
    21481 ggacgctaat gctgctcatc aacaaccacg aagtggaaaa gctcttcgat atgaaggcgt
    21541 gtcttgcggc tctggaagat gcgtacgacg atctgctcat cggcgatgcg gtttatcggc
    21601 cgcgtttgga tctctggatg ccttgtgacc ggccggacgg ttattggcgt tggggaacga
    21661 tggaaggcgc cagccggaag atcggcgtgt ttgcgattcg gatgaaatcc gatgtcgttt
    21721 actggcccaa cggcgacacc gaagagaaat attgcatgca gccgggtacc tggtgcgggc
    21781 tggtgatggt tttcagcgtg cgcaacggcg agccgctggc gattatcaat gacggtttga
    21841 tgcagcatat gcgggtgggc gggtgcgctg gactgggcgt aaagtattta gctcgcgagg
    21901 atgcctccga cgtcggcatc ttcggttcag gcggcatggc gcgcacctat ctggaggcgt
    21961 tttatgaagt gcgtaaactg aaaacagtca gagtttacag tccgaccaaa gcaaatcgag
    22021 aaagcttcgc gcgggagatg actggcaaat tggatttgga agtggttgcc gtcgactcgc
    22081 cggaaaagct ggtgcgcggc gccgatatca tagcaacgtg cacggattca actcgaacgg
    22141 ttttcgatga gccggagtgg ttgaaaccag gagcgcatat tacttgcgtg cgcgcctgcg
    22201 aggtcgggcc aaaggtcgtc aaaaaatgcg atctgtcggt caagcttggc aagaacacga
    22261 tcgaagcgat ggatgaaggc atgatccgcc tgcatggcaa tgtcggctac atcgccggac
    22321 agcccgaaga gcgcagccgt atccccaatc ccaccgtaga caattataag ggcgactact
    22381 tcaaatactt catggatgtt cgggccggaa aggtgccggg acgaatcagc gatcagcaac
    22441 tgactttttt catcaacgcg ggcacccagg gacttcagtt cgccgcctgc gccggaaagg
    22501 tcgtccaatt agccaaacaa aagaaggtgg gccgtgagct acccaccgaa tggtttttgc
    22561 aggatattcg ggactaagcg ccgaacgatc ccggagttaa gacgctgttt gtaatgacgc
    22621 gggcagtgtc tcttttgcgc atgtaaccga tcatgcgctt tgatccggcc tgcacctgct
    22681 tgaatcaact gcagcaaatg cactgacctt taaacaatct gctgcctctc aaaccagcgg
    22741 cagtgatcgt ttgctcattg cttaaaaatc agacatcttc catcaccaga gtagatagcc
    22801 atgcaaaccg gcgaattctt caatctatat aatcacggtc tgatccgggt cgcggtcggt
    22861 attccggaag tacaagtcgc agatccggct ttcaacggca gccagaccat tgagcttatg
    22921 aaaagggcgg agcatgacca cgcgattctg gcgctctttc cggaactagg gctgtcggcc
    22981 tactcctgcg acgacttgtt tcatcagcag gcgctgctcg atggttcgtt ggggtccctg
    23041 cgctctatcc tcaaggtttc tgaagcgctg aacctcattg cagtggtggg tatgccgctg
    23101 caagtcgacc acctcttgtt caattgcgcg gttgtgctct ctcgcgggag gattcttgga
    23161 atcgttccta agacgttcct gcccaattat cgggagtatt acgagtaccg gcagtttgct
    23221 ccggcggctg cagcttgcag caccgaggtg gagattttcg atcaaccggg catccctttt
    23281 ggggcgcacc tgcttttcca ggccgagagg cagcgcgagt ttacgttctg cgtggaaatt
    23341 tgtgaggatc tctgggtgcc gattccgcct tcctcgtatg cggcgttagc gggcgccact
    23401 attatcttga atctctcagc ttcgaatatc accatcgcta aggacgagta ccggcataat
    23461 ttagtcgcca accagtcggc tcgctgtatc gccgcatacc tctataccgc ggcaggcacg
    23521 ggcgaatcga ctactgatct tgcatgggac gggcacgcaa tcgtttatga cagtggcgtc
    23581 ctagtcagcg aatccgaacg gttccgttac gactctcagc tggtgttggc ggaactcgat
    23641 ttggagcggc tggcgctcga gcgcatgcgt cagaacacct ttggccagag catgcagctg
    23701 caccgcgaag agcttcgtcg gtttcgcaag gttcagttcg atctcgagct gccgaaaact
    23761 ggccgcctgt tgtgcagccg aaggattgat cggttcccct acgttccttc ggatcccgcg
    23821 gtgcgcgacc ggcgctgcta cgaggcgtac aacattcaag tgcagggact cgctaagcgg
    23881 ctcaggagtt ctaacatttc taaaatcgcc atgggtattt ctggcgggct tgattcgact
    23941 cacgcgctcg tggtggctgc gcgagcaatg gatgtgctga aactgcctcg ttcgaatatt
    24001 ttggcctata ccatgcccgg cttcgccacc agccagcaca ctttggagaa cgccaatgcg
    24061 ctgatggccg ctatcggttg cgaatcgcac gaattggaca ttcggccgag ctgcgagcag
    24121 atgctgcgag atatcggcca tccctatgcc gccggacaac cggttttcga tgtcaccttc
    24181 gaaaacgttc aggcgggaga acgcaccagc catctttttc ggctcgctaa tcttcatcga
    24241 gcattggtgc tgggcaccag tgacttgagc gagctggcgc tgggttggtg cacctatggc
    24301 gtgggagatc acatgtccca ctacgccatc aatgcgagtg tgccgaaaac tctgattcag
    24361 tttctcattc gctggg
//
LOCUS       DASUVS010000470        12733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1739530, whole genome shotgun sequence.
ACCESSION   DASUVS010000470 DASUVS010000000
VERSION     DASUVS010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739530"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VF182_24695"
     CDS             <1..260
                     /locus_tag="VF182_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX6440340.1"
                     /translation="SEPAVLIQAHLHQARMLIIAVPDTVDVRRMVEMARTVNPRIAAV
                     VRTHRDEQLTSLENQIAGKVFLGEHELAVSMTRYVLEAYEK"
     gene            complement(521..970)
                     /locus_tag="VF182_24700"
     CDS             complement(521..970)
                     /locus_tag="VF182_24700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX6440341.1"
                     /translation="MKFQIPTSLKLEHEELHAELARATKAGGKTGEAAKAVAAVLHNH
                     FLKEEEFALPPIGLLAPVARGEIDKEMRAVLTMTDRLKAELPEMLREHQAVVAALEEL
                     SAAAQKEKLPEHARFAEKLMLHAKTEEEVLYPAAVLIGEYLKLKLKN"
     gene            complement(1030..1620)
                     /locus_tag="VF182_24705"
     CDS             complement(1030..1620)
                     /locus_tag="VF182_24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HEX6440342.1"
                     /translation="MNKTFTTPSIVSILLFTIALFLPKQVMAHCDGLDGPVVKAAQTA
                     LENGNVNPVLIWVQKKDEGEIKQAFQKTLAVRKLNLQAKELADMYFFETLVRIHRAGE
                     GEPYTGLKPAGRDLEPAIPAADKAIESGKLEPLLKILTETLQTEVREKFKAVSAKKNF
                     KPDNVDAGREYVEVYVPFVSYVERIYATAKGAAKGH"
     gene            complement(1764..2567)
                     /locus_tag="VF182_24710"
     CDS             complement(1764..2567)
                     /locus_tag="VF182_24710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6440343.1"
                     /translation="MTRTPSKTPLKKLSLQPGIIYGPVQSRRLGRSLGINLLPADYKL
                     CSFNCLYCQYGWTKNVTFTPGDRLKDLPSPDAVTTALENTLAEFSRGDKAIDAISICG
                     NGEPTLYPALGEVIAKIKKLRDRYQPDARTAILSNSSTVGDPAVRVALALLDLKIMKF
                     DAGSEEMFRQLNHPAAPVYMGEIISGLKELENILLQACFVQGRVTNADPDSVAMWLEK
                     LREIHPLGVQVYTLDREPADKRIERVSAATLSWIADAVRWRSRLKVEIF"
     gene            complement(2592..3743)
                     /gene="acd"
                     /locus_tag="VF182_24715"
     CDS             complement(2592..3743)
                     /gene="acd"
                     /locus_tag="VF182_24715"
                     /EC_number="1.3.99.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaryl-CoA dehydrogenase Acd"
                     /protein_id="HEX6440344.1"
                     /translation="MDFELPEELAEVQKLAHDFADKEIAPGAAQDDREHRFRKDLVGK
                     MGELGFYGCLIPEEFGGNGLGFLAHTLISEEIARVHSAIRVYLNMQAGPAVTLFEFGN
                     EEQKKKYLPGLLSGESIGLFAITEPDAGSDVAAMKTTAKRDGQHYLLNGTKIWITNAT
                     VADAGVIFACTDRSQKHRGISAFYADLNQPGVTRRDLDKLGAHASPTGELTFDNFAIP
                     AESLIGREGDGFKICMRHLNHTRLGCAAGAIGLAKAAREAAVNYANQREQFGEKIGQF
                     QMNQDLIAQMIVQEEAARLLVYRAACLADRGRPYNLEVSIAKYAAAEAAAFCADTALK
                     ILGAYGYSTEFPVERYYRDAKSYQIVEGSVNIQKLIIAQDALGYRRANR"
     gene            complement(3733..4893)
                     /locus_tag="VF182_24720"
     CDS             complement(3733..4893)
                     /locus_tag="VF182_24720"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6440345.1"
                     /translation="MEFLDDDQSRLLRQKVHSWTGKNPLDVEPSAETLERAACELAQG
                     LGREGLMAYAIPRPHGGMRETVQARDLCVIREELASHAALADVMFGVQALGSYPIVIA
                     GTEEQKKKYLPALASAERLAAFALTEPEAGSDAAAINAEAVRVSGGYRLHGVKRFISN
                     AGIAQVYIVFASTTPGAKAKGISAFIVPSDAPGFTVKEKTRLLSPHPIGVLAFDDCFV
                     PQEQRLGAEGDGLNIAYRTLDTLRSTVGAAAVGFARRALAEAISYSQSRRQFGRPIGD
                     FQGIQFKIAEMAADLEAARLLVYQAAWAHDTEDPELSLKSSIAKLFATEAAQRIVDHA
                     LQIHGGNGVVAGHIMERLYRDVRALRIYEGSSEIQKIVIAKKLMHKERPDGL"
     gene            complement(4903..5352)
                     /locus_tag="VF182_24725"
     CDS             complement(4903..5352)
                     /locus_tag="VF182_24725"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6440346.1"
                     /translation="MKKEIYVRNLEKRDLPAIVSMEERQTGIARPEYWEKRIEISEAI
                     RPHWASLVAEIDNRVVGFVLGRAGEFEFGLPGTIAWIEIIGVDPAYRAQGIAQELVGQ
                     FAESAEDHGIKTIFTLISNTQDEMQQFFSKQGFVHGKMLHYQKELSG"
     gene            complement(5376..6170)
                     /locus_tag="VF182_24730"
     CDS             complement(5376..6170)
                     /locus_tag="VF182_24730"
                     /inference="COORDINATES: protein motif:HMM:NF006107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEX6440347.1"
                     /translation="MSYSSFVFENNDGVATIRLNDPERLNALTFQTYADLEKITAELA
                     HDPTVKVVVLTGTGKGFCSGGRVDDIIGPLLKMKGDELYKFTRMTCNVIKNMRSLKKP
                     IIAAVNGIAAGAGAMLMLASDLRLFSDKARAAFLFVKVGLSGADMGALYLLPRIVGLG
                     KATELVYFGDTIDAQEAYRIGLANRVVPGDTLMDEAYKWANRLKDGPLYALGVTKELL
                     EYEANVDLETALQMEAMAQARCMETPDFIEGYNAFVEKRPLKFNRS"
     gene            complement(6203..6982)
                     /locus_tag="VF182_24735"
     CDS             complement(6203..6982)
                     /locus_tag="VF182_24735"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6440348.1"
                     /translation="MKDLAGKIVLITGAARGIGKAIALAFGAEQSRVVITGRTAATLE
                     EVAAVIRSTGAEVLARQCDVTQKAQVESLRQEIEKRFGAVEILVNNAGLAPAADFLEM
                     ADELWDEVMRVNVNGAYNCCKTFLAPMIGARWGRIINIASTVARVAYPGIAAYATSKH
                     ATLGLTRALAMETARSGVTVNAICPGYVNTELTRETARRLAEKRGQSLEQALAMLAAS
                     CPQRRLIEPEEVAYATLMLASDRARGITGQAIQVDGGAVMA"
     gene            complement(6986..7765)
                     /locus_tag="VF182_24740"
     CDS             complement(6986..7765)
                     /locus_tag="VF182_24740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6440349.1"
                     /translation="MHQTLHYREDEAVATIRLHRPEKKNSLNAEMRHEMASLLKELAR
                     KPAIRAVVITGGDDIFCAGADIGEIGGPADAESSYHHAREFQILFDQVEALPQPVIAA
                     VSGFALGGGCELAIAADFRIASETARFGLPEIKIGAFPGGGGTQRLQRLIGVAKAKEM
                     ILTGDPIGAQEALAAGLVMKVVPAGKCLEEAASLAARLAALPRLALQASKMLINRSQE
                     LDLATGLEFEARTFAAIAHTHDLAEGTRAFLEKRKANFTGN"
     gene            complement(7821..8066)
                     /locus_tag="VF182_24745"
     CDS             complement(7821..8066)
                     /locus_tag="VF182_24745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440350.1"
                     /translation="MCRFLSADFEYDLKCPVDHQNHTVRFQAYPELRGHALDVVECDG
                     KSRVDQFTCGKACHGLLESGEFWQRIYPESATFARSQ"
     gene            complement(8083..8940)
                     /locus_tag="VF182_24750"
     CDS             complement(8083..8940)
                     /locus_tag="VF182_24750"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6440351.1"
                     /translation="MRNDIKAIDFMYYVSTPEFIAKWTEAKKGELLCRMEKAIGGLPQ
                     YDSIEAMIAKMDQAGVEKVFITQCKMWSYRNKWMYMDTQLEEVAQYTKRYPDRFVGLA
                     SYNPFRIKESLQELDQAIKVYGFKGVYIHIYGFDIPLHDAKMYPLYAKCAELDVPVSM
                     QVGHVLEGMPSEHARPIYLDRIASDFPDLKIIGAHTGWPWVEELISVCYKWENVYFGV
                     DAWMPKYMKPEIIHYINSRMGQDRCLWGTNGLPWKESLDQWAEVGLKEATKKKLLRDN
                     AVELFGLSH"
     gene            complement(9021..9782)
                     /locus_tag="VF182_24755"
     CDS             complement(9021..9782)
                     /locus_tag="VF182_24755"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX6440352.1"
                     /translation="MKLQGKTALIAGGGGGIGRAVALAMAREGANSAVADIVKDNAEK
                     VCAEAKELGVEVLACQVDLTRKADVDRMVNDVLARFGSVDILVNCQGWDRLEPFVESH
                     EETWEKLLAINFKSVLYTAKAVLPQMISHGGGKIVNISSDAGRVGSSWEAVYAGAKGA
                     VIAFSKTIAREVARYKINVNVVCPGLTETPLLQAVRSQSEQTARIIEAVTKATPFRRP
                     AKPEEIAEAVLFFTSPAAEFITGQTLSVSGGLTMV"
     gene            complement(9779..10615)
                     /locus_tag="VF182_24760"
     CDS             complement(9779..10615)
                     /locus_tag="VF182_24760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEX6440353.1"
                     /translation="MTFVAGIDIGAKSTKAVIMDGDRRVCGKASVKTRPDFAAIAKEA
                     LDGALQQAGRKESDLNYIATTGFGRYNVPFRDVQITDITCVGRGAVFLFPKTRCVLDI
                     GAQSTRALRVSDTGKVREFRTNDKCAAGAGGFIERACKYLEVAIDQVGDLSIQANKPQ
                     AISSVCAVLAESEIINHVSQGESVENILRGVHNSLASRALALLKRSGMEDEVTFVGGV
                     ARQKGMVKALEETLKRKINVSDEPELVGALGAALLALRRLEKIREDKKTAVLGEEARA
                     AS"
     gene            complement(10650..11480)
                     /locus_tag="VF182_24765"
     CDS             complement(10650..11480)
                     /locus_tag="VF182_24765"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEX6440354.1"
                     /translation="MIYAAGIDVGSTQTKGIIVNDKLEIVARALTDTGAYVTRAAERC
                     FVEALRISQIKREDVGYVVGTGYGRYKVMFGDAQITEISCHARGASYLFPSTRTVIDM
                     GGQDAKGIKIGIDGDVKDFVMNDKCAAGTGRFLASAAEALGMTLGDIGDISLQAKNPV
                     RLTTVCTVFVESDIMSYLAQGKKTEDILGGVHSAIAARTISLVRRVGIEPEVTFTGGV
                     SLNIGMVRALEEKLGMKLNVSPDSHYVGALGASIFALERATKSVSESQKVAGGEAVGA
                     "
     gene            complement(11477..>12733)
                     /locus_tag="VF182_24770"
     CDS             complement(11477..>12733)
                     /locus_tag="VF182_24770"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HEX6440355.1"
                     /translation="MHLLQKELMGRYFERVTRAAEKGEGKAVYMLISGNPVELVQAFD
                     LIPVYPEIDALQLAVKKSALPFIQKAEELGYSMDNCAYVKADMGMFLGDRKTPFATIP
                     LPSLILCNYVGCNVYLQWFEHLAEYCEGQLYNLDIPFLRDPSGQPSPEDITYVVRQLE
                     EVIEICEKLTGKKLDYARLKDIVRRSSETGEMWSRIKGLTKNLPSPFDAYFDSVTMMA
                     PLYCLRGTEEGVRFFETALNEMEEKARMGTGPLPEERFRFIIEGPPPWPYLRTFRDMF
                     SKWGAVAVASTYSTVGGLWEFGFRHDPDRPLESIAAHMLQWNVTNRNFLQRYQQIRRY
                     VEEWHADALVIHSVKSCRLFSAGQGDMREYFTKQLNVPTLLIESDLEDPRYFSEAQLK
                     NRIDAFFESLEHKKMVRAGAEAGGVA"
BASE COUNT         2557 a         3743 c         3511 g         2922 t
ORIGIN      
        1 cctccgagcc cgccgtgctc attcaagcgc atctgcatca ggcgcgcatg ctgataatcg
       61 ccgtgcccga tacggtggac gtgcgccgca tggtcgagat ggcgcgcacc gtaaatccgc
      121 gcatcgcagc ggtagtgcgc acgcaccgcg atgaacagtt gacctcttta gaaaaccaga
      181 tcgccggaaa ggtgttcctg ggcgagcacg agcttgccgt gagcatgaca cgatatgtcc
      241 ttgaagcgta cgagaaatga ccgacggcga ggggggaggt cgacagagtg gatttaagta
      301 ggattctggt cgcgaccgat ttgtcgccgc gcgttgagaa gacaataacg cgcgcgttca
      361 ggtggccgat aagcacggag cagcacggac tatccttcat gtgttgaccg ccgcggcagg
      421 agttaagctc cgtcaccggc aggtcgctct acaagcaacc gctgcgaaag cgcccgcggc
      481 atcccgagat ggcaggatcc gttgcacgtt ggatattatt ctagttcttc aatttgagtt
      541 tcagatactc gccgattaaa acggcggcgg gataaagcac ctcctcttcc gtttttgcat
      601 gcagcatcaa cttttccgca aagcgggcat gctccggcag tttttctttt tgtgctgcgg
      661 cgctgagttc ttcaagcgcg gccactaccg cttgatgttc ccgaagcatt tccggcagct
      721 cggctttgag ccggtcggtc attgtcagaa cagcgcgcat ttctttgtcg atttcgccgc
      781 gcgcgaccgg cgccaaaaga ccgatgggtg gaagagcaaa ttcctcctcc ttcaagaaat
      841 ggttatgaag aactgcagcc acggctttcg ccgcctcgcc ggttttgccg ccggccttcg
      901 tcgcccgggc aagctccgcg tgtagctcct catgttccaa ctttaatgac gtgggtattt
      961 gaaatttcat gcgtagacct ccgggttttc ggtcccttct tcggccaatc ctgctttcgt
     1021 cgattcgtgt caatgcccct tggccgcgcc tttggcggtg gcgtagatcc gctcgacata
     1081 tgaaacgaaa ggaacgtaca cctccacata ctctcgccct gcatcgacat tatccggctt
     1141 gaagtttttc ttggctgaga ccgctttaaa tttttcgcgc acctctgttt gaagagtttc
     1201 ggtcaagatt ttgagcaatg gctccaactt gccgctttct atcgccttat ccgccgcagg
     1261 gattgccggt tcgaggtcgc gcccggcggg ctttaatccg gtatagggct ctccttcgcc
     1321 agcccggtga atacgcacaa gtgtctcgaa aaaatacata tctgctagtt ctttcgcctg
     1381 gagattgagc ttacgaacag caagcgtctt ctggaaggct tgttttatct cgccttcgtc
     1441 cttcttctgt acccaaataa ggaccgggtt cacgttccca ttttctagtg ccgtctgggc
     1501 ggccttaacc actgggccgt ccagtccgtc gcaatgcgcc ataacctgct tgggcaggaa
     1561 aagagcaatc gtgaaaagaa gaatcgaaac gatggatggg gtagtaaagg tcttattcat
     1621 cacaggtcct ccttcagaaa tgttccaccc ggataattgc tgtagctact tcattgagcc
     1681 ataacatcgt caagaaggca gccgctcacc agagccggaa gttgcgaagg tgacacgcat
     1741 aagtgaggga agttttgccg agctcaaaat atttccactt taaggcgcga gcgccagcgt
     1801 accgcgtcag cgatccaact cagagtggcc gcactgactc tttcgattct cttatcagcg
     1861 ggctcacgat ccaacgtgta gacttgaact cccagcggat gaatttcccg gagtttctcg
     1921 agccacatcg ccacggaatc cggatcggcg ttggtgaccc gcccctgcac gaaacaggcc
     1981 tgcagcaaga tattctcgag ttctttcagc ccggagataa tttcacccat gtaaaccggc
     2041 gccgccggat gattgagctg gcgaaacatc tcctcgctgc cggcatcgaa tttcataatc
     2101 ttaagatcga gtagcgcgag cgcgacgcga acggcgggat ctccgacggt cgaagaattc
     2161 gacaggatcg cagtccgggc atcgggttgg taacggtcac ggagcttttt tatcttagcg
     2221 atgacctcgc ccagggccgg atagagagtt ggctcgccgt tgccgcagat cgaaatggca
     2281 tcgatcgctt tatcgccgcg tgaaaactcg gccagggtgt tctccagcgc cgttgtcacg
     2341 gcatcgggcg acggtaagtc cttcagtcga tcgcccggcg tgaaggttac gttcttcgtc
     2401 cagccatact ggcagtaaag acaattaaac gagcacagtt tgtaatccgc ggggagcaaa
     2461 ttgatgccaa gggatctgcc caaacggcgg gactgcacgg ggccatagat gatccccggt
     2521 tgtaaggata attttttcag cggcgttttc gacggtgtac gggtcatatc gagtatgaca
     2581 actcgtggtg ttcagcgatt cgcccttcgg tagcccagcg cgtcttgcgc gatgatgagc
     2641 ttttggatgt tcacggatcc ttcgacgatc tgataagact tggcgtcgcg ataataacgc
     2701 tcgacgggaa attccgtcga gtatccatag gcgccaagaa tcttcagcgc cgtgtcggcg
     2761 caaaaagccg cggcttctgc ggccgcgtac ttggcgatgg aaacttcgag attgtaaggc
     2821 cggccgcggt cggccagaca ggcggcgcgg taaaccagca gacgggcggc ttcttcctgc
     2881 acgatcatct gcgcgatcag atcttgattc atttgaaatt ggccgatctt ctcaccgaac
     2941 tgctcgcgct gattggcata attcaccgcc gcctcgcgag cggccttggc cagtccgatg
     3001 gcgccggcgg cgcaaccgag ccgcgtgtga tttaagtggc gcatgcagat tttgaaacca
     3061 tcgccttccc ggccgatgag actctctgcc ggaatcgcaa agttatcgaa ggtcaattcc
     3121 cccgtcgggg aggcatgcgc gcccagcttg tcgaggtccc ggcgggtcac gccgggctga
     3181 ttcaaatcgg cgtagaaagc cgatatgccg cgatgtttct gcgaacgatc ggtacaggca
     3241 aagatgactc ccgcatcggc cactgtggcg ttggtgatcc agatcttggt gccgttgagc
     3301 agataatgtt gaccgtcgcg cttggcggtg gtcttcatcg ccgccacatc cgatccggcg
     3361 tccggttcgg tgatggcaaa taaacctatc gattcgccgc tcaacaggcc cggcaaatat
     3421 ttcttttttt gctcttcatt gccgaactcg aagagggtta cggcgggacc ggcctgcata
     3481 ttgagataaa cccggatggc gctgtggacc cgcgcgattt cttccgagat cagcgtgtgg
     3541 gcgagaaacc ctagaccgtt gccgccgaac tcttccggaa tcaagcaacc gtagaaaccc
     3601 agctcaccca ttttgccgac caagtctttg cgaaagcgat gttctcggtc atcttgcgcg
     3661 gcgcccggcg cgatttcctt gtcggcgaaa tcgtgcgcga gtttttgcac ctcggcgagt
     3721 tcttccggca actcaaagtc catcaggacg ctccttgtgc atcagctttt tggcaatcac
     3781 gattttttgt atctcggaag atccttcgta gatgcgcagg gcgcgcacat cacggtaaag
     3841 ccgttccatg atgtgccctg ccaccacgcc gttaccacca tgaatctgca atgcatggtc
     3901 gacgattctc tgggcggcct ccgttgcaaa gagcttggcg atggatgact tgagcgacag
     3961 ctccgggtcc tccgtgtcat gggcccaggc ggcttgatag acgagcaggc gcgccgcctc
     4021 caagtctgcc gccatctcgg cgatcttgaa ttgaattcct tggaaatcgc cgatcggtcg
     4081 gccgaattgg cgccggctct ggctataaga gatcgcttca gctaacgctc tgcgcgcgaa
     4141 acccacggca gcggcgccga ccgtcgagcg caaggtgtcc agagtgcgat aagcgatgtt
     4201 cagcccatcg ccttcggcgc cgagccgttg ctcttgcggc acaaaacagt cgtcaaaggc
     4261 caggaccccg atcggatgag gtgaaagcaa tcgggttttt tccttgaccg taaagccggg
     4321 agcatcggac ggcacgataa aggcgctgat gcctttggcc ttggcgcctg gtgtggttga
     4381 cgcgaacacg atgtaaacct gagcgatccc cgcgttcgag ataaagcgct tgacgccgtg
     4441 caagcggtaa ccgccgctga ctctgacggc ctcggcgtta atcgccgcag cgtctgatcc
     4501 cgcttcgggt tcggtcaagg cgaaggcagc gagcctttcc gcgctcgcca gagccggcaa
     4561 gtattttttc ttttgttctt ccgtgccggc gatgacaatg ggatagctgc cgagagcctg
     4621 aaccccgaac atcacgtccg ccagggctgc atgcgatgcc aattcctcgc ggatcacgca
     4681 cagatcgcgc gcctgcactg tttcgcgcat gcccccgtgg ggacggggaa tcgcatacgc
     4741 catcaagccc tcgcggccga gtccctgggc cagttcgcaa gcggcgcgct cgagcgtttc
     4801 cgcgctgggc tcgacatcaa gcggattctt gcccgtccag gaatggactt tttggcgcag
     4861 aagccgggat tggtcgtcgt ctagaaattc catgatcgct gtttaaccgg aaagctcctt
     4921 ttgatagtgc agcatcttgc catggacgaa accctgcttg ctgaaaaact gctgcatctc
     4981 gtcctgggtg ttgctgatca gggtaaaaat ggtcttgatg ccgtggtctt cagccgattc
     5041 agcgaattga ccgacgagtt cctgggcaat cccttgggcg cggtacgctg ggtcaacccc
     5101 gatgatttcg atccacgcta tggtgccggg taatccgaac tcaaattcac cggcgcgccc
     5161 caacacaaag ccgaccacgc ggttgtcgat ctcggcgacc aaagaggccc agtgcgggcg
     5221 aatcgcctcg gagatttcga ttctcttctc ccagtactcg ggacgcgcga tcccggtctg
     5281 gcgctcctcc atgctgacga tcgcaggtag atcgcgcttc tcaagatttc ttacatagat
     5341 ttcttttttc atatctgttc tccaactctc atgcttcagc tgcggttgaa ttttaacggc
     5401 cgcttttcga caaaggcgtt gtaaccctcg atgaaatccg gtgtttccat gcaccgcgcc
     5461 tgagccatgg cttccatctg gagcgcggtt tctaaatcca catttgcttc gtattcgagc
     5521 agctccttgg tgacacccag cgcatagagc ggcccgtcct ttaaccggtt agcccacttg
     5581 taggcttcgt ccatcaaggt gtcacccggc acgacccggt tggccaagcc gatgcgataa
     5641 gcttcctggg catcgatggt atcgccgaag tagaccagct cggtggcttt tcccagcccg
     5701 acgattctcg gcagcaaata gagcgcgccc atatcggcgc ccgatagtcc cacttttacg
     5761 aatagaaacg ccgcccgcgc tttgtccgaa aagaggcgca gatccgacgc cagcatgagc
     5821 attgcgccag cgccggcggc aatgccgttg accgccgcga tgatcggctt tttcaagctg
     5881 cgcatgtttt tgatcacatt gcaggtcatg cgggtgaatt tataaagctc gtctcctttc
     5941 atcttgagca gcggcccgat gatgtcgtcg acccggccgc cggaacaaaa acctttaccg
     6001 gttcccgtga gcacaacgac cttgaccgta gggtcatggg ctaattccgc ggtgattttt
     6061 tccaggtcgg cgtaagtctg aaaagtcagg gcattgagcc tttccggatc gttgagccgg
     6121 atggttgcga cgccgtcgtt attctcgaaa acaaaactgg aataactcat tcaatggttc
     6181 tccttgcagt tgtcgtttct gttcaagcca tgacggcgcc gccgtcgact tggatcgcct
     6241 ggccggtgat gcctcgggcg cgatccgagg cgagcatcaa ggtcgcatag gcaacttctt
     6301 ccggctcgat cagacgccgt tgcggacagc tcgccgccag catggcgagc gcctgctcca
     6361 gcgattggcc gcgtttctcg gcgagtcgcc gcgcggtttc ccgggtcaat tccgtattga
     6421 catagccagg gcatatcgcg ttcacggtga ctcccgatct cgcggtttcc atagccaacg
     6481 cgcgagtcag gccgagagtt gcgtgtttag atgtcgcata ggcggcaatc cctggataag
     6541 caacccgggc caccgttgaa gcgatgttga tgatgcggcc ccagcgggcg ccgatcatgg
     6601 gtgccagaaa cgttttgcag cagttgtagg cgccgtttac attgacacgc atgacttcat
     6661 cccacagctc gtctgccatc tccaagaaat ccgctgccgg cgcgaggccg gcattgttca
     6721 ccagaatttc aaccgcgcca aaacgttttt cgatctcctg ccgaagactt tccacttgcg
     6781 ctttttgcgt cacatcgcat tggcgcgcca atacttcggc gcctgtggag cgtatcactg
     6841 ccgcgacttc ttcaagggtc gctgccgttc tgccggtaat gactacccgg ctttgctcag
     6901 cgccaaacgc cagggcaatc gctttgccga tgccgcgtgc cgcgccggtg ataagcacga
     6961 tttttccagc gagatccttc atctgttaat tgccggtaaa attcgccttg cgcttctcca
     7021 aaaaggctct cgtcccctca gccaaatcat gcgtatgcgc gatcgcggca aatgtgcgcg
     7081 cttcgaattc gagtccggta gccaagtcca gctcctggct ccgattgatc agcatcttcg
     7141 atgcttgtaa cgcaaggcgg ggcagcgccg ccagcctggc tgccaggctg gcggcctctt
     7201 ccaggcactt gccggcaggc actaccttca tcaccaagcc tgcggcgagc gcttcctgcg
     7261 caccgatcgg atctccggtc aaaatcatct ctttggcttt ggcaacgcca atcaagcgct
     7321 gcaatcgttg cgtgccgccg ccgccgggaa acgcgccgat cttgatctcc ggcagtccaa
     7381 aacgcgccgt ctccgaagca atacgaaaat cggcggctat cgccaattcg cagccgccgc
     7441 ccagggcgaa tcctgagacc gccgcgatca ccggttgggg cagtgcctcg acttgatcga
     7501 agaggatctg aaactcgcgc gcatggtggt aagaagattc agcatcggcc ggccctccga
     7561 tctcgccgat gtccgcgccg gcgcaaaaaa tatcatcgcc gccggtgatc acgactgccc
     7621 gaatggcggg tttgcgcgcc agctctttca gcaagctcgc catttcgtgg cgcatctcgg
     7681 cattgagcga atttttcttt tcgggccggt gcaggcgaat cgtcgcaacc gcttcgtcct
     7741 ctcgatagtg taaggtttga tgcatacttg ggttggctcc aatcatgggc gtcttcgatc
     7801 ggtcactgac ttaaacatga ctactggcta cgcgcgaaag tagccgactc cggataaatt
     7861 ctttgccaaa actcgccgga ttcgagcagc ccgtggcagg ctttgccgca ggtaaattgg
     7921 tcgactcgag attttccgtc acattcgacc acatccaatg catggccgcg gagctccgga
     7981 taggcctgga atctaacggt gtggttttga tgatcgaccg ggcatttcaa atcgtactca
     8041 aaatcagcgg ataaaaatcg gcacatggcg gcctcctttg gatcaatggc tcaaaccgaa
     8101 gagttcaacg gcattatcgc gcaagagttt tttcttggtg gcttctttca gtccgacctc
     8161 cgcccactga tccagactct ctttccaagg caagccgttg gtcccccaca ggcaacgatc
     8221 ctgtcccatg cggctgttga tgtagtgaat gatctcgggc ttcatgtatt tgggcatcca
     8281 ggcatcgaca ccgaaataaa cgttttccca cttgtagcag acagaaatca gctcttcgac
     8341 ccaaggccag ccggtatgcg cgccaatgat tttgagatcc ggaaaatcac tggcgatacg
     8401 atccagataa atcggccggg catgctcact gggcattcct tccaggacat ggccgacttg
     8461 cattgatacc ggcacatcca gctccgcgca tttcgcgtag agcggataca ttttggcgtc
     8521 gtgcagcggg atatcgaagc cgtagatatg aatatagacg cccttgaatc catacacttt
     8581 gatcgcctga tcgagctcct gcagcgactc cttgatgcga aatggattgt agctggcaag
     8641 accgacgaag cgatccggat agcgtttcgt gtattgcgcc acttcctcga gctgggtgtc
     8701 catgtacatc cacttgttgc gatacgacca catcttgcat tgagtgatga agactttttc
     8761 tactcccgcc tgatccatct tggcaatcat ggcctcgatg ctgtcatact gcggcaggcc
     8821 gccgatggcc ttttccattc ggcacaggag ctcgcctttc ttcgcctcgg tccacttggc
     8881 gatgaattca ggcgttgaca cgtagtacat gaaatcaatg gccttgatgt cgtttcgcat
     8941 ctctttctcc tctcacccgc gctcgtcgtt gctcgtgctc acggcaaaca cgagacacga
     9001 tcacgaacac gccatggcgt tcaaaccatc gtcaatccgc cgctcacgct caaggtctgt
     9061 ccagtgataa actccgccgc cggagacgtg aagaacaaca cggcttcggc aatttcttcg
     9121 ggcttggccg gacggcgaaa gggtgtggcc ttggtgaccg cctcgatgat tctcgcggtc
     9181 tgttccgatt ggctcctgac ggcttgcagc agcggggtct ctgtcaaccc ggggcacacc
     9241 acattgacgt tgatcttata acgtgccact tcgcgggcga tggtttttga aaacgcaatc
     9301 accgctccct tagcgccggc atagaccgct tcccagctgg agccgacccg gccggcgtcc
     9361 gagctgatat tgacgatctt cccgccgccg tgagagatca tctggggcaa gaccgctttg
     9421 gcggtgtaaa gcacggattt gaagttgatc gccagcagtt tctcccacgt ctcttcatgg
     9481 ctttccacga acggctccaa tcgatcccag ccttggcagt tgacaaggat atcgaccgag
     9541 ccgaagcgcg cgagaacatc gttcaccatg cgatcgacgt ccgctttcct ggtcagatcg
     9601 acctggcagg cgagcacttc cacgccaagc tcctttgcct cggcgcagac tttctccgcg
     9661 ttgtccttga cgatatcggc gaccgcgctg tttgcgcctt cgcgtgccat ggccagcgcc
     9721 accgcgcggc caattcctcc accgcctcct gcgatcaggg cggtttttcc ttgaagcttc
     9781 atgacgctgc tcttgcctcc tctccgagca cggcagtttt cttatcctcg cgaatcttct
     9841 ccaggcgacg tagcgccagc agagccgcgc ccaaagcgcc gaccagctcc ggctcgtcgc
     9901 taacattgat tttgcgtttg agtgtctcct ccagcgcctt gaccatgcct ttctgccgcg
     9961 cgacaccgcc gacgaacgtg acctcgtctt ccatgcccga gcgtttgagc agggctagcg
    10021 cccgagatgc cagcgaattg tggacgccgc ggagaatgtt ttcgacggac tctccttgag
    10081 agacgtgatt gataatttcc gattccgcca ggacggcgca gacgctgctg atggcctgcg
    10141 gcttgtttgc ctgaatcgag agatcgccga cctgatcgat agcgacctcg agatatttgc
    10201 aagcgcgttc gatgaagccg ccggcgccgg cggcgcactt gtcgttagtg cgaaactcgc
    10261 ggactttacc ggtgtcgctc acacgcagcg cgcgcgtgct ttgcgcgccg atgtcgagca
    10321 cgcaacgggt cttgggaaag agaaaaactg cgccccgtcc gacgcaggta atatcggtga
    10381 tctggacatc gcggaaagga acgttgtagc ggccgaagcc ggtggtggcg atgtagttga
    10441 ggtcactctc tttgcgcccc gcctgctgca gcgctccatc cagcgcctcc ttggcgatcg
    10501 cggcgaagtc tggccgcgtt ttcacgcttg ctttgccgca aacgcgcctg tccccgtcca
    10561 tgatgactgc cttggtcgat ttggcgccga tatcaatgcc agcgacaaaa gtcatgatgc
    10621 ctccttaata ggccttaggg cattaggcac taagccccca ccgcctcgcc ccctgctact
    10681 ttttgcgatt cgcttaccga cttggttgcg cgttcgagag cgaagatgct cgccccgagg
    10741 gcgccgacat agtgggaatc cggacttacg ttgagcttca tgccgagttt ttcttcaagc
    10801 gcgcgcacca tgccgatgtt gagcgaaacc ccgcccgtga aagtcacttc gggttcgatg
    10861 ccgacgcggc gtaccagcga gatggtgcgg gcggcgatgg cgctgtgcac gccgccgagg
    10921 atgtcctcgg tctttttgcc ttgagcgaga taagacatga tgtccgactc gacgaacacg
    10981 gtgcatacgg tcgtcaggcg caccggattt ttcgcctgaa gcgagatatc gccgatatcg
    11041 ccgagggtca tgcccaaagc ttcggcggcg ctggcgagaa accttccagt gccggcggcg
    11101 cacttatcgt tcatgacgaa gtctttgacg tcgccgtcga tgccgatttt gattcctttg
    11161 gcgtcctgcc cgcccatatc gatcacggtg cgggtgctgg gaaagagata gctcgcgccg
    11221 cgcgcatgac agctgatctc ggtgatttgc gcgtcgccga acatgacttt gtatctgcca
    11281 taaccggtgc ccaccacata accgacgtct tcacgtttaa tttgggagat ccttagcgct
    11341 tcgacgaagc atctttccgc ggctctggtg acgtaggcgc cggtatcggt cagagctctg
    11401 gcaactatct cgagtttgtc attgacgata atacccttgg tctgagtgga gccaacatct
    11461 attcctgcgg cgtagatcat gccacacctc ctgcttccgc tcccgcacga accattttct
    11521 tgtgctccaa tgattcaaag aaagcgtcga tgcggttctt gagctgcgct tcggaaaagt
    11581 atcgcggatc ttcgaggtcc gattcgatca acagagtggg tacgttcaat tgcttggtga
    11641 agtattcacg catatctccc tgtccggcgg agaacagccg gcagctcttc acggaatgaa
    11701 tcaccagagc atccgcatgc cactcttcca cataacggcg gatctgctgg taacgctgca
    11761 ggaagttgcg gttggtgacg ttccactgga gcatatgcgc ggcaatcgac tccagcggcc
    11821 ggtcgggatc gtgcctgaag ccgaattccc acaaaccgcc gaccgtggaa tacgtcgaag
    11881 ccactgccac cgcgccccat ttggaaaaca tatcgcgaaa ggttcgcaga tagggccagg
    11941 gcggcggccc ttcgatgatg aagcggaagc gctcttcagg caaaggcccg gttcccatgc
    12001 gtgctttctc ttccatctca ttgagggcgg tctcgaaaaa cctgacccct tcttcggttc
    12061 ccctgaggca gtagagcgga gccatcatag ttacggaatc gaagtaggcg tcaaaaggag
    12121 aaggaaggtt cttggtcaaa cctttgatgc gcgaccacat ctcgccggtt tcactcgaac
    12181 ggcggacgat gtctttcagg cgagcgtagt cgagcttttt cccggtgagt ttttcgcaga
    12241 tctcgatcac ctcttcgagc tggcgcacga cgtaagtaat gtcctcgggg ctcggttgtc
    12301 cggagggatc gcgcaagaac gggatgtcga gattgtagag ttgcccctcg caatattcgg
    12361 ccagatgctc gaaccactgc aggtaaacat tgcagccgac gtagttgcag agaatcagcg
    12421 acggcaatgg gatggtcgcg aacggagtct tgcggtcgcc gagaaacatg cccatatcgg
    12481 ctttcacata agcgcagttg tccatcgagt aacccaactc ttcagctttc tggataaagg
    12541 gcagcgcgct cttcttaacc gctaattgta aggcatcgat ctccgggtag accggaatca
    12601 agtcgaaagc ttggaccagc tccactgggt tgccgctgat caacatatag acggctttgc
    12661 cctcgccctt ttccgccgcc cgggtaacgc gttcgaaata ccgccccatc agctcctttt
    12721 gcagcagatg cat
//
LOCUS       DASUVS010000471         6863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1740378, whole genome shotgun sequence.
ACCESSION   DASUVS010000471 DASUVS010000000
VERSION     DASUVS010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6863
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740378"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..461
                     /locus_tag="VF182_24775"
     CDS             198..461
                     /locus_tag="VF182_24775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440356.1"
                     /translation="MFEPDRKNPAQQRAAQTYLRLARASCPDGSATITREPLGMAFAT
                     NCCATIGVGEGIVHPEDSELQIIPPEFLAYVGFAKVIFFYSNA"
     gene            479..1426
                     /locus_tag="VF182_24780"
     CDS             479..1426
                     /locus_tag="VF182_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012408474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX6440357.1"
                     /translation="MAESKTAIYAAIAGNLAIAVTKFTAAAFTGSSAMISEGIHSLVD
                     TGNGGLLLLGIRMSKKPADASHPFGYGKELYFWTLIVAMLIFGVGGGVSIYEGILHLL
                     HPTPLNDPIWNYVVLGLAVVFEGVSLFIAYREFQVVKGQHSIWQAIRASKDPTTFTVL
                     FEDSAAMLGLIAAATGIFLGHRFENPYFDGAASIVIGLILAAVALFIGYESKGLLIGE
                     GADPVVLQSITRIAESDPKIARVERPLTMYFGPHTVLLTMDVQFRNGLSGADRDATVK
                     RLEEAIREKHRDINHIFIESKSRSPRAEPQQQGHESAAG"
     gene            1480..2478
                     /locus_tag="VF182_24785"
     CDS             1480..2478
                     /locus_tag="VF182_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6440358.1"
                     /translation="MDKAEVVVITGASAGVGRATARAFARRGAKIGLLARGRDGLEGA
                     RREIETAGGKALVLPTDVASPEQIEAAAEAVEENLGPIDIWINNAMVSVFSPIKEMTP
                     DEFKRVTDVTYLGAVYGTLSALRRMLPRDRGVIVEVGSALAYRGIPLQAAYCGAKHAV
                     QGFVDSLRTELLHDQSRVRVTMVQLPALNTPQFSWVKSRLPRKPQPVPPIFQPEVAAD
                     AIVWATLHDRPELYVGWPAVKAIVGNKIAPRFADWYLSRQGYEAQQTDEPVEAGRVDN
                     LWEPVPGDHGAHGDFGARSRSSSWQLRLAKHKTSFTIAALAVAAALMALAKDTRRA"
     gene            2521..3690
                     /locus_tag="VF182_24790"
     CDS             2521..3690
                     /locus_tag="VF182_24790"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEX6440359.1"
                     /translation="MKPILMISLLACFFSACEKQEAKAPAPQATPPAVEEKKTASAEK
                     PPPAAAPGSYADVVERAGPAVVTIRAAKRVRAPQQHPFLNDPRFRDFFGGIFPGAAES
                     GPRVELGLGSGVIVSSDGTILTNHHVVDGAQEIQVELTNHRLFSAKVIGSDAPSDLAV
                     LKVDGKDLAVLALADSDRVRVGDVALAIGNPLGVGQTVTAGIISAKGRATGLSDGSFE
                     DFLQTDAPINQGNSGGALINSRGELVGINSQILSPSGGNIGIGFAIPSNMARNVSDQL
                     IKGGKVRRGQLGIAIEPVTPELASKLGMQEVRGVMVGALSPNSPAARAGIREGDVILA
                     FNGTPVTDGNTLRNQVASTAPGTAVKVTVTRDGREQEIEVTLGEYQPPKVAQRRR"
     gene            complement(3802..4170)
                     /locus_tag="VF182_24795"
     CDS             complement(3802..4170)
                     /locus_tag="VF182_24795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6440360.1"
                     /translation="MNSGANLKILLVDDHQIACKSTALLLEMSGYKVRTAFNGCTAIE
                     IAVDFSPDFIILDIGLPDIDGYELLSRMKRFEKLRASKFIALTGFEEEMARSSQTGFD
                     FHHFLRKPVDTTQLKALLNA"
     gene            4450..5229
                     /locus_tag="VF182_24800"
     CDS             4450..5229
                     /locus_tag="VF182_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015203440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6440361.1"
                     /translation="MSIDSTDFDQYSSTSLDQDRRGARSQNMSETERWVSLGVGVGLL
                     ALGLKQGIRDGLMLVITGGALLYRGATGQCPLYKVAGIDTSSDYPRPGVSVPHGRGVK
                     VENTVLINKSPEELYRFWRQFENLPRFMENLVSVKTEGDQRSHWVVKAIGNAEISWDA
                     EIVNDVPNELIAWRTVENSDVDHAGSVRFEGDERGTRVKVTTEYRPPAGKIGVGLAKL
                     FGQEPRQMIESDLRRFKQLMESGEVLSVEGQPHGSSVDRSI"
     gene            complement(5407..>6863)
                     /locus_tag="VF182_24805"
     CDS             complement(5407..>6863)
                     /locus_tag="VF182_24805"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX6440362.1"
                     /translation="AATSTSHEQGLMMPVADICELNLTELSKLLATRELSSTEAVKAA
                     LTRLERLDGKLNAFITVPREQAIAEAKKADDEIAAGRYRGPLHGVPVSIKDLFETAGL
                     RTTAGSKIFADWIPETDSAVVQRLRSAGAVIIGKTNLDEFGHGGTSTLSHFGPVHNPW
                     NIEHIAGGSSGGSAAAVAAGIGPLSYGTETGSSVRRPASYCGIVGFKPTFGIISRTGS
                     FRGAWSQDHVGLFARSVRDITLGLDPVAGFDARDPASVHQDTAAYTARLDAHVKQLKI
                     GILRRFLEGVNDEVSQAFETAVQVFVRAGCEIVDLDVPEVSYAAMTSMMTSSAESAGI
                     NRRWFHERYQDFVPHVSRGIAVGMTITASEYLTVQRARHRIREALRKTYEQVSIIASP
                     TTARVAPLISDGVKGNVDDTRHASYNHSNLLRFPSMLGLPGCSIPCGINTAGLPIGMQ
                     LIGAWFADQMVLNAALAFQNATDWHSRRPAIASA"
BASE COUNT         1515 a         1829 c         2027 g         1492 t
ORIGIN      
        1 actcgtgttt gcagcatggt ccagcccctg agccacgaag acaagataac caagtcgtga
       61 ataaataacc gctcgtccgc agcgaatttt gcgaatggcg agagactgaa agcccatgcg
      121 cagcgtgcgc ctaacgtacg agaggcggcg tgcgaacacg agctgcacgg gtaactccga
      181 cggtcggcga atctcctttg tttgaaccag atcgcaagaa tcccgcgcag caacgggcag
      241 cgcaaactta tttgcgtttg gcacgtgcat cctgccctga cggctcggcc actatcacgc
      301 gagagccgtt aggcatggcg tttgcgacaa attgttgcgc cacgatcggt gtaggagaag
      361 ggatcgtcca tccggaagat tccgagctgc aaattatacc gccggagttt cttgcgtatg
      421 tcggattcgc caaggtcatt tttttctaca gcaatgcttg acgagactgc gatttaacat
      481 ggctgaatcc aagacagcga tctacgccgc gatcgccggt aatctcgcaa tcgcggtaac
      541 caaatttacc gcggccgcat ttaccggtag ttcggcgatg atatcggaag gaattcactc
      601 gctcgtcgat accggcaatg gcggattact gctactcggc atacgcatga gcaaaaaacc
      661 cgccgatgcc agtcatccat ttggttatgg caaagagctc tacttctgga ccttgatcgt
      721 ggcgatgctg attttcggcg tcggcggcgg cgtcagcatc tatgaaggca ttttgcatct
      781 gcttcatccg acgcctttga acgatccaat ctggaattac gttgtcttgg gtctggcggt
      841 ggtattcgaa ggcgttagct tgtttatcgc ttaccgagag tttcaggttg tcaaaggaca
      901 gcacagcatt tggcaggcga tccgcgcgag caaggatccg accactttca cggtgctttt
      961 cgaagactct gccgccatgt tgggtctgat tgccgcagcg acaggaatct ttctcgggca
     1021 tcgattcgaa aatccctatt tcgacggcgc cgcttcgatc gtcatcggac tgatactggc
     1081 agccgtggct cttttcatcg gctacgaaag taagggtctt ctaatcggtg aaggcgccga
     1141 tcccgtagtc ctgcaaagca taacgaggat cgcggaatcg gatccaaaga tagcaagggt
     1201 cgagcggccg ctgacaatgt atttcgggcc gcatacggtc ttattaacga tggacgtaca
     1261 atttcgcaat ggtttatcag gagccgatcg agatgcgacg gtgaaacggc tggaagaagc
     1321 tattcgcgaa aagcaccgcg acatcaatca tatcttcatc gagtcaaaat cgcggtcccc
     1381 tagagcagaa cctcagcaac aagggcacga gtcagccgca ggttaaaccc gcccgctggc
     1441 gccggcgttg cgaagaatta cacggtgaaa tgaggagata tggacaaagc tgaagtggtc
     1501 gtgattaccg gggcctcggc gggtgttggg cgggctaccg cgcgcgcgtt cgcccgccga
     1561 ggagcgaaaa tcgggttact cgcacgggga agagatggct tggaaggggc gcggcgtgag
     1621 atcgagacgg caggcggcaa ggctttggtg ttgcccaccg atgtggcgag ccccgagcag
     1681 atcgaagccg ctgctgaggc ggtggaagaa aaccttggac cgattgatat ctggatcaac
     1741 aacgccatgg tttcggtttt ttcgccgata aaggaaatga ctccggacga gtttaaacgg
     1801 gtgacggatg ttacttatct tggcgctgtc tatggaacgt tatccgcgct gcgacgcatg
     1861 ctgccgcgcg accgtggcgt catcgtcgaa gtcggttccg ctctggcgta tcggggtatc
     1921 ccgctgcagg ctgcctattg tggagccaag cacgccgtcc agggattcgt cgattctttg
     1981 cgtaccgaac tactgcacga tcaaagtcgt gtgcgggtga ccatggttca gctgccggcg
     2041 ctcaacacgc cgcaattttc gtgggtcaaa agccggctgc cgcgcaagcc gcaacccgtg
     2101 ccgccgattt ttcaacctga agttgccgct gatgcgatcg tctgggcaac gcttcatgat
     2161 cgtcccgagc tttacgtcgg ttggccggcg gtcaaggcca tcgtcggtaa caagattgcg
     2221 ccgcgctttg ccgattggta tctgtctcgg caaggctatg aagcgcaaca aaccgacgag
     2281 ccggtcgaag cagggcgtgt cgataattta tgggagccgg ttcccggcga tcatggcgcg
     2341 cacggagatt tcggcgctcg ctctcggtct tcaagttggc agctccggct cgccaagcat
     2401 aagaccagct tcaccatagc ggcgctggcg gttgccgctg cgctaatggc gcttgcgaaa
     2461 gacactcgcc gggcatgacc gataagatca aggcgtgccg cgcctaattt ggagagatct
     2521 atgaaaccca ttttaatgat tagcctactt gcttgcttct tctccgcttg cgaaaagcag
     2581 gaagctaaag cgccagcgcc gcaagccacg ccgccggcgg tcgaggagaa gaaaaccgcc
     2641 tctgctgaaa aaccgccgcc tgccgcggcg ccgggatcgt acgccgatgt ggtcgagcgt
     2701 gccgggcccg cggttgtcac gatccgggca gccaagcgtg tgcgggcgcc gcaacagcac
     2761 ccgtttttga acgatccccg ttttcgtgat ttttttggcg gcatttttcc aggggccgca
     2821 gagagcggcc cgagagtgga gctgggcttg ggttcgggag ttatcgtatc ctcggacggc
     2881 acgattctga ccaatcatca cgtggtcgac ggtgcccagg aaattcaggt tgaactgacc
     2941 aatcaccgcc tatttagcgc aaaagttatc ggctccgacg cgccgagtga tcttgcggtc
     3001 ttgaaagtcg atgggaagga tctcgctgtc ttggcattag ctgattcaga tcgcgtgcgt
     3061 gtgggcgatg tcgcgctcgc cataggtaat ccgctgggtg tgggccaaac ggttacggcg
     3121 ggaattatca gcgccaaagg gcgtgcgacc gggctgagcg atgggagttt cgaagatttc
     3181 ttgcaaaccg atgcgccaat caatcagggt aattcgggtg gcgcattgat caacagccga
     3241 ggcgagctgg tcggcataaa ttcgcagatc ttgtccccct cgggcggcaa catcggtatc
     3301 ggctttgcca ttccctcgaa tatggcccgc aacgtgtcgg accagttgat caagggggga
     3361 aaggttcgtc gcggccaact cggcattgca attgaaccgg taaccccgga gttggcctct
     3421 aagctcggta tgcaggaagt tcggggcgtc atggtcggcg cgctgagtcc aaacagcccg
     3481 gcggcgcgag cgggcattcg cgaaggcgac gttattctag cttttaacgg gaccccggtg
     3541 acggacggaa atacgctgcg aaatcaagtg gcgagcaccg cgccgggaac ggcggtgaaa
     3601 gtcacggtaa cacgggatgg tcgcgagcag gagatcgagg tcaccttggg cgagtatcag
     3661 ccgccaaagg tggcacaacg ccgcagatag ctggttcttt aactgaaaac tcaacgaagc
     3721 gggagtgtgg agaatataac tttaacggac atcttcgaac cacactaata tcggacaatt
     3781 agcgggtttg ccgttgccgg attaagcgtt gagaagagct ttcagttgag tagtgtccac
     3841 gggcttgcga agaaaatggt ggaaatcgaa tccagtctga cttgatcgag ccatctcttc
     3901 ttcgaacccg gtcaaggcaa taaacttaga ggcccgaagt ttctcgaacc gtttcattcg
     3961 actcagtagt tcatagccat cgatatccgg aagtccgata tcgagtatga tgaagtcagg
     4021 ggagaagtct accgcgattt ctatggctgt gcagccattg aaagcggttc gaaccttgta
     4081 gccggacatc tccagcaaga gcgctgtgga cttgcaggcg atttggtggt catcgactaa
     4141 aaggatcttt agattagccc cactattcat gctaaatgct tccactcacc atcgtgtctc
     4201 actagataga tcacaagcca ggctgcatgg agatcttatg cgcataatat gtgtcctatt
     4261 tttcggattg caagtcccat ccgtaaacat tttcttcaaa gcgtgcgcca ggcttccatg
     4321 catcagatga aatttagctt agttcgtcga gttataaaaa taacccgcgc tggcataacg
     4381 cttgctgaat gtaggcgcac cggcgccgga gtcaattgtg agtagccaat cccaaaatga
     4441 ggagaaccaa tgagcatcga tagcacagat ttcgatcagt actcgtccac atcgctggat
     4501 caggatcgga gaggcgctcg atcgcaaaat atgagtgaga ccgaacgttg ggtctcactc
     4561 ggagttggtg ttggactttt ggctttagga ttgaagcagg gaattcgcga cggcctgatg
     4621 ctcgtcatca ccggcggtgc cctactttat cgcggcgcga ccggacagtg tccgttgtat
     4681 aaagtggctg ggatcgatac gtcatccgat tatccgcgtc ctggtgttag cgtaccgcac
     4741 ggacgcgggg tgaaggtgga gaataccgtg ctcatcaaca agtcgccgga ggagttgtat
     4801 cgcttctggc gccaattcga gaacctaccg agattcatgg agaatctggt ttctgtaaaa
     4861 accgaggggg accagcgctc gcattgggtt gtcaaagcga ttggcaacgc ggagatctcc
     4921 tgggatgccg agattgtcaa cgatgtgccc aatgagctga tcgcctggcg tacagtagag
     4981 aattccgatg tcgatcacgc cggttcggtg cgctttgaag gagacgagcg cggcacccga
     5041 gtgaaagtca ccacggagta tcgcccgccg gcaggcaaga tcggtgtagg cctggctaag
     5101 cttttcggcc aagaaccgcg gcagatgatt gaaagtgatt tgcgccgttt caaacagctt
     5161 atggagtcag gtgaagtctt gtccgtcgaa ggacagcctc acgggtcgag cgtcgatcgc
     5221 agcatataag acgctgtcga tagccaaaat agcagattat taaactttga catcgtgccc
     5281 attaaatggt ccacagattc ctcatctgtt tctctggaac agatgagcgc ggccgtgacg
     5341 ccaccgcgaa ccgttaccca ttaacttgtt taactcctgc gcatcgttgc gcgtgatcga
     5401 cattcgctaa gcactagcga ttgcaggacg gcggctgtgc caatcggttg cattctgaaa
     5461 tgccaaggct gcgttcagta ccatctgatc agcgaaccaa gcgccgatca gttgcatgcc
     5521 gatgggcaac cccgcggtgt tgatgccgca cggaattgag cagcctggca ggccgagcat
     5581 gctcggaaag cgcagtagat tagaatgatt gtagctggcg tggcgcgtgt cgtcgacgtt
     5641 gcctttgaca ccatccgaaa ttaacggcgc gactctggct gtggtcggcg aggcgataat
     5701 actgacctgc tcataggttt tccggagcgc ttcacgaatt cggtgccggg cgcgctgcac
     5761 ggttagatat tccgaggcgg tgatcgtcat tcctactgcg atacctcggc tcacgtgcgg
     5821 cacaaagtcc tgataccgct cgtgaaacca gcgccggttg atcccagcgc tttcggccga
     5881 gctggtcatc atcgatgtca tcgccgcgta gcttacctcg ggaacatcca agtcgacgat
     5941 ctcgcatcct gcgcggacaa agacttgaac tgccgtctcg aatgcctggc tcacttcgtc
     6001 attgacgcct tcgagaaaac gccggaggat gccgatttta agttgcttga cgtgggcgtc
     6061 gagccgcgca gtatatgcgg cggtatcttg gtgaacgctg gccggatcac gcgcatcgaa
     6121 acccgccacc ggatccagac ccaacgtaat atctcttact gaacgcgcga acaggccgac
     6181 gtgatcctgg ctccaggcgc cgcggaacga gccggtccgg ctaatgatgc cgaaggtcgg
     6241 cttgaaaccg acgatgccgc agtacgatgc gggtcggcgc actgacgaac cggtctcggt
     6301 gccgtaggag agcgggccga ttccggccgc aaccgcagcc gccgagccgc cgctggatcc
     6361 accggcgata tgctcgatgt tccagggatt atgaaccggc ccaaaatggg acagcgtcga
     6421 cgtcccgccg tggccgaact catccaggtt ggttttcccg ataatgaccg ctccggctga
     6481 gcgcaggcgc tgcactacag ctgagtccgt ttcgggaatc caatccgcga aaattttcga
     6541 tcctgcggta gtgcgcaggc ccgcggtctc gaacaagtct ttgatactca ccggtacccc
     6601 gtgaagggga ccgcgatagc gcccggcagc gatctcgtca tcggctttct tggcttcggc
     6661 tatcgcctgc tcgcggggga ccgtgatgaa cgcgttgagc ttgccatcga gacgctccag
     6721 ccgagtcaga gccgccttga ccgcctccgt ggatgacagc tcgcgagtcg caagaagctt
     6781 ggaaagttcg gtcagattca attcgcagat atcagcgacc ggcatcatca agccttgctc
     6841 gtgactggtt gaagttgctg cga
//
LOCUS       DASUVS010000472         6667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_209429, whole genome shotgun sequence.
ACCESSION   DASUVS010000472 DASUVS010000000
VERSION     DASUVS010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209429"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..671)
                     /locus_tag="VF182_24810"
     CDS             complement(12..671)
                     /locus_tag="VF182_24810"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6440363.1"
                     /translation="MLYKEIVFLITVVLCSGCSSLQTAGEMQSGRQALLAGNMERALP
                     YFQEVANKNPNYLYEAVLFRESIWTYLGRSQYALGMLEPARASFERALSVYRDDNLAR
                     IYLGLTLARTGDRSAAVKELQSGMQGLHDWLDYMERSRPFTAYWDPLREIRAELEKNV
                     QMIAGKDIAWDQLISSGEWIGKRMEEEIDNVRRDERRQFERQDRDGRGSGTALGIGIG
                     F"
     gene            927..1400
                     /locus_tag="VF182_24815"
     CDS             927..1400
                     /locus_tag="VF182_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008985364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6440364.1"
                     /translation="MPQVKAIPDEYRGATPYLSVTGAAEAIEFYKKAFGARETVRMPA
                     PAGKVGHAEIRIGEAAIMLADESPEMNFRSPQTLGGSPVNILIYVNDVDAVVNRAVAA
                     GAKLLRPVADQFYGDRMGVLQDPFGHTWSFATHIEDLSAEELQKRAAQHAGCTER"
     gene            complement(1599..2732)
                     /locus_tag="VF182_24820"
     CDS             complement(1599..2732)
                     /locus_tag="VF182_24820"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6440365.1"
                     /translation="MKDGFKVIDADRHILEPSDLYQKYLPEKFKSRVRLEGPNQTVRL
                     VDGQPVSDSALRPGRVTEDFGYIFSSSKRWRECFADAYANKFDPASNVRDMDREGIDV
                     SVLFPTIGLYIMWRDDMDPELSAAICRAYNTWLAEYCDYDRKRLHGVMLIPLQDPARA
                     VEELKYAREKLGLVGIFWRPNKFCGRTLASSEYDSVYAAASDLGVTVCVHEGARTVLP
                     QAGSDRYSEFGRHIACHPLEQMLACLNFCADGVLEKFPKLKVAHLESGCGWVPFWLER
                     MDEHWEHESHGSAKLTKEKPSYYFKRQCWVSCEAGEELAPTFIEHVGDDYLMIATDYP
                     HSDAIDKFPDKTVGAISSNQEIPAESRRKILWDNPTKTFGLGA"
     gene            complement(2854..4674)
                     /locus_tag="VF182_24825"
     CDS             complement(2854..4674)
                     /locus_tag="VF182_24825"
                     /inference="COORDINATES: protein motif:HMM:NF022518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEX6440366.1"
                     /translation="MALSEDVAAKIVLIRSIEECDPRAFSEELRARAFVAAQDVEPGL
                     SSIERYAACLFEHLSPWYQSILQLAKIPGPWTLPVCFTAFLLGLATNLLGPARQIHVI
                     RNPIFILIAWNLLVFLAIFILFFRHKIKKPQLSKVVPAQKRAGADKLESLSSNLPNNP
                     SQAPWVVKYLLPGLWHFFHRMIFAVHEHKNLAHILRRFSQHWLSVAGALVVSRWRRLL
                     HLGSVFVATGAVAGMYFRGLFQGYRVVWDSTFIVKQEAVATFIRALFGPSLWLSNLFG
                     LDLANQISIGRLLTPAGDEADGWIHLFAISVLVAVVLPRLALSAWESTRIDRLSKNIG
                     LPLDRYYGEVIEGPIRLLIEKEVAAQAQRFSAEVADFVASKLYDEQIVPKLVSFRQAG
                     GRVSDLKAELTSMTVGFLPKIQSFITDGAVPKMQAALSQRLGEVLKSIGTSFIDVKDP
                     QIAFEEVSIFPAGRTELKISNEFSRAIAFSIGTSIALAVATVGGGIGEQLGVAIIATV
                     LGTTGPIGFLIGLIAGAVVAAGAWWFGKDKITETVEHVLLPAAVVRAALWQSRFQRLL
                     AQAREKCEEAVRAKVDEKLQTIIPQITDDILLRFRRLRQY"
     gene            complement(4775..>6667)
                     /locus_tag="VF182_24830"
     CDS             complement(4775..>6667)
                     /locus_tag="VF182_24830"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX6440367.1"
                     /translation="LRVWAVPGKKHLAKYAEQIGSFSLTHFAEYYGIPYPGDKLDLIA
                     IPDFASGAMENLGAITFRETALLVDSEKATRGELERVADVVSHENAHMWFGDLVTMKW
                     WNGLWLNEAFATFMEMLAVDAWKPEWRRWESFTVSRAAAMQIDGLKSTRPIEFKVEKP
                     EEAAGMFDVLTYEKGASVLRMLEQYLGAEPFRDGIRLYLKRHQYANAETTDLWDAIED
                     STHQPVRALMDTWIFQPGYPVITIAKRSGQIVLSQRLFRYLVDDEGSKQKWLVPIFLR
                     AGTKHGVVTRTVLLADQELVVDLPDEPDWVVVNAGGHGFYRARYSDELMSGLKQALQS
                     ILSSVERFGLVNDSWAATVAGLTPLFEYLNLLDLLRDERDINVWTTVIGSAHHLNRIL
                     EEAQRTELQGRLRAVFAPAVQRIGWTVRQGESELESQLRGDLIAAFGTVAADKSCQAR
                     ARELYSQYERSATAVDRNLVPALISIVAHTGTPADYEKFYGKFKNAQTPQEEQRYLFA
                     LAGFRQPDLIDHTCRLTINGEVRTQNSPYLMRNLLLNPDARSRGWDFMKEHWDEMLRQ
                     YPDNSIPRMCEGIIGLATPDLETDVKDFFARHPVKQGAKQMEQHLERLHISVACRERW
                     YKHGLI"
BASE COUNT         1543 a         1939 c         1772 g         1413 t
ORIGIN      
        1 aagctaaacc ttcagaagcc gatgccgatg ccaagcgccg tgcccgagcc gcggccatct
       61 cggtcctgac gctcgaactg gcggcgttca tcgcgccgca cattgtcaat ttcctcctcc
      121 atccttttac cgatccattc gccactggag attaactgat cccacgcgat atctttgccg
      181 gcaatcattt gaacattttt ttccaactca gcgcgaatct cacgcagcgg atcccaatag
      241 gctgtgaaag gacggctgcg ttccatgtag tccaaccaat cgtgcagtcc ctgcatgccg
      301 gactggagct ctttcactgc cgccgaccga tcgccggtgc gcgccaaggt gaggcccaga
      361 tatattctgg cgaggttgtc gtcccgataa accgaaagcg cccgctcaaa cgatgcccgg
      421 gcgggttcca gcattccaag agcatactgg cttcggccga gataagtcca gatggactcg
      481 cggaaaagca ccgcctcata cagataattc gggttcttat tggcaacctc ttggaagtac
      541 ggcagggccc gttccatatt gcccgcgagc aacgcttgcc ttcctgattg catctcccct
      601 gcagtttgaa gcgacgaaca gccggagcac aacactacgg ttatcaggaa aacaatttct
      661 ttatagagca tggggtgacc ttctattcaa cacgaacgac ggatcagaac agttcagagc
      721 tgcagcaatc ggcaaatcga acgacatagc cttcggcgat acccagtcaa accccatcta
      781 acagctcaac aaagaaggag tcaattaagg caccggcgcc tcggaagatc ggaaccgata
      841 acttgaagct cacctggatt gctatcacct cgtgaagata tatctttaaa aactagagta
      901 atacgaggga tcaaggaggg aagataatgc cgcaagtaaa agccattcct gatgagtacc
      961 ggggcgccac cccatatctg agcgtcaccg gcgcagccga ggcgatcgag ttctacaaaa
     1021 aggctttcgg agcacgtgag acggtgcgta tgcccgcgcc ggcgggaaag gtaggccacg
     1081 ccgaaatcag aatcggcgag gcggccatca tgcttgccga tgagtctccg gagatgaatt
     1141 tccgcagccc gcagacgctt ggcggcagtc cggtaaacat ccttatttat gtcaacgacg
     1201 tcgacgcggt ggtgaaccgg gcggtggcag caggcgcaaa gctgctacga ccggttgctg
     1261 atcaattcta cggcgaccgc atgggcgtct tgcaggaccc gttcgggcac acatggtctt
     1321 tcgccaccca catcgaggat ttatctgcag aggagctgca aaaacgcgct gcacagcacg
     1381 ccggctgcac cgaacgataa cagtaatgat gggcgccggt agacttaccc agcatcaaca
     1441 catcggcgtg attttgaggc gggaaaaatc tttctcggta gcgtaaaccg gtcatgtagc
     1501 gcgaaatccc ggacagctta agctggagag ctcatgacga gcgtcaagaa aaagcccggg
     1561 ttgtcgcccg ggcttttttt tcgataagtt ttcggatgct atgccccaag cccgaaagtc
     1621 ttagtcggat tgtcccagag tattttgcgc cgcgactcgg cggggatctc ctggttgctg
     1681 ctgatcgcac ccacggtttt gtccgggaat ttatcgatgg cgtcgctgtg tgggtagtcg
     1741 gtggcgatca tcaagtaatc gtcgccgaca tgttcaatga atgtgggcgc taactcttct
     1801 ccggcctcac agctcaccca gcactgccgt ttgaagtaat aactgggttt ctccttggtc
     1861 agcttggctg agccgtgcga ttcgtgctcc caatgttcat ccatgcgctc gagccagaac
     1921 ggtacccagc cgcagccgga ttcgaggtgg gcgactttga gtttgggaaa tttttccagc
     1981 acgccgtcgg cgcagaaatt aagacacgca agcatctgct ccagcgggtg gcaggcgata
     2041 tgtcggccaa actcgctgta acggtcggag ccggcttgcg gcagaaccgt gcgcgcacct
     2101 tcatggacac aaacggtaac acccaagtcg gacgccgccg catagacgga atcgtactcc
     2161 gagcttgcca gtgtgcgccc gcagaatttg ttcggccgcc agaagatgcc gacgagtccc
     2221 aatttttcgc gcgcgtattt taactcctcg accgctcgcg ccgggtcctg cagcgggatc
     2281 aacatgaccc catgcaggcg tttgcgatcg tagtcgcagt attcggccag ccaggtgtta
     2341 taagcgcggc aaatcgcagc gctcagttcc ggatccatat catcgcgcca catgatatag
     2401 agcccgatgg tcggaaacag gacgctgaca tcgatgccct cgcgatccat atcgcggacg
     2461 ttggaagccg gatcgaattt gttagcatag gcatcggcga aacattcgcg ccatcgtttt
     2521 gatgaggaaa aaatatagcc gaaatcctcc gtcacccgac cggggcgcag cgccgagtcg
     2581 gacaccggct gtccgtcgac caagcggacc gtctgattgg gcccttcgag ccgcacccgg
     2641 cttttgaact tctcgggcaa atacttttga tagaggtccg agggttcgag aatgtgccgg
     2701 tcggcatcga tgactttgaa accatccttc ataactgtct ccttcctgca ctgctgagga
     2761 tcttgataat actagtcagt actgcctttc ataaatcaat ccggtcgcgt cagcaagtgg
     2821 ccggccaagg tcggcgccgc aaatgtgtcg cgctcaatac tgccttaggc ggcggaacct
     2881 caacaaaatg tcatcggtaa tctgtggaat tatcgtctga agcttttcgt ctactttcgc
     2941 gcgcacggcc tcttcgcact tctcacgcgc ttgggccagc agtcgctgga accgggattg
     3001 ccatagggcc gcccgtacta ccgccgccgg cagcagaacg tgctcgaccg tttccgtgat
     3061 cttgtccttg ccgaaccacc acgctccggc agcgaccacg gcgcctgcta tcagcccgat
     3121 taaaaaaccg atgggtccag tggtgcccaa aaccgtggcg ataatagcga cgcccagttg
     3181 ttctccgatg ccgccgccaa ccgtggctac ggcgagcgca atcgatgtgc ctatagaaaa
     3241 cgcgatggcg cggctaaatt cattggaaat cttcagttca gttctgcccg cgggaaatat
     3301 cgacacctcc tcaaaagcta tctgcggatc tttgacatcg ataaagcttg tgccgatgct
     3361 ctttaacacc tcacccagtc gttgtgacaa cgccgcctgc atcttgggaa cggcaccatc
     3421 cgtgatgaag gattgaatct taggcaagaa gccgacggtc atactcgtca actccgcctt
     3481 gagatcggaa actcttccgc cggcttgacg aaacgagaca agctttggaa caatctgctc
     3541 atcgtaaagt ttcgatgcga cgaaatcagc cacctcggcg gaaaatcttt gtgcttgcgc
     3601 cgcgacctcc ttctcgatca ataaccgaat cggtccctcg ataacttcac cgtagtaacg
     3661 gtctaatggc agcccgatat ttttggacaa gcgatcgatc cgggttgact cccaagccga
     3721 caacgcaagt ctcgggagca ccacggcaac gagaaccgag atcgcgaaca gatgaatcca
     3781 gccatccgcc tcatcgccgg ccggagttaa gagccggccg atgctaattt gatttgccag
     3841 atcaagaccg aacaaattgg aaagccacag gctggggccg aacagcgcgc gaataaacgt
     3901 tgcgaccgct tcctgcttga cgatgaaagt actgtcccac accactcgat aaccttgaaa
     3961 cagcccgcga aaatacattc cggcaacggc tccggtcgcg acaaagaccg atccgagatg
     4021 aagcaagcgc cgccatcgtg aaaccacgag tgctccggca acggagagcc agtgctggct
     4081 gaaacgtctc aatatgtgcg ccaggttctt atgttcatga actgcaaaaa tcatccgatg
     4141 aaaaaaatgc cacaggccag gcaacaagta tttaactacc cagggcgctt gagatggatt
     4201 gttcggcaga ttactgctga ggctctcaag tttgtcggcg cccgcccgct tttgagcggg
     4261 cacgaccttc gagagttgcg gtttttttat cttatgacga aagaacagaa tgaaaatcgc
     4321 caggaacacc agcaggttcc aggcgatcaa aataaaaatt ggattgcgga tgacgtgaat
     4381 ttgtcgcgcc gggccaagca gattggtcgc caggcccaga aggaaagcgg taaaacacac
     4441 cggcagagtc caggggcctg gaattttcgc cagctgcagg atggattgat accaaggcga
     4501 gagatgttcg aacaagcacg cggcatatcg ctcgatggaa cttaacccgg gctccacgtc
     4561 ctgtgcggcg acaaatgctc gagctcgaag ttcctctgaa aacgcccgcg gatcacactc
     4621 ttcgatcgaa cggatcagta cgatcttggc ggcgacgtct tcgcttaaag ccatggttga
     4681 ctaggctcga gtcagaagca tctcagccga acccttggaa gtattgcaaa aaatatcaat
     4741 cgaacgagcc tgcgctgcat ctccgccatt ctgattagat taagccgtgc ttataccagc
     4801 gctcccggca ggcaaccgaa atgtgcagtc gctcaagatg ctgctccatc tgctttgccc
     4861 cttgctttac cggatggcgc gcgaaaaaat ccttcacatc cgtttcgagg tccggcgttg
     4921 ccaaaccgat aatcccttcg cacatgcgcg ggatcgagtt gtccgggtat tggcgcaaca
     4981 tctcgtccca gtgctctttc ataaagtccc agcccctcga gcgggcgtcg ggatttagca
     5041 ataggttgcg catcaagtac ggcgaattct gcgtccgcac ctcgccgttg atcgtcaggc
     5101 ggcacgtgtg atcgattaaa tccggctgcc ggaagccggc gagagcaaac agataacgtt
     5161 gctcttcctg cggcgtctgg gcgtttttga acttgccgta aaacttctcg tagtccgccg
     5221 gcgttccggt gtgggcgaca atcgagatga gcgccggcac taggttgcga tccaccgcgg
     5281 tagcgctccg ttcatattgg gagtagagct ctcgcgcccg cgcctggcag cttttgtccg
     5341 ccgccaccgt accgaaagcg gcgatcaaat ccccgcgcag ctgactttcc aattcgcttt
     5401 ccccttggcg caccgtccag ccgatgcgtt gcacagccgg cgcaaacacc gcccgcagcc
     5461 gtccttgcaa ttcggtacgc tgcgcttctt cgaggattcg gttcagatga tgagcggagc
     5521 caatcacggt tgtccataca ttgatgtcgc gctcgtcgcg gagcagatcg agcagattga
     5581 gatactcgaa aagcggcgtt aaacccgcga ctgtcgctgc ccagctatcg ttgaccaacc
     5641 cgaaccgttc gaccgaagac aagatgcttt gcagcgcttg tttcaggccg ctcatcaatt
     5701 catcgctgta gcgcgcacga taaaagccgt gtccgccggc attcaccacc acccaatccg
     5761 gttcatcggg caggtcaaca acaagctcct ggtcggcgag caggacggtc ctggtgacaa
     5821 caccgtgttt agtgccagcg cgcagaaaga tcggcaccag ccacttttgc ttcgatcctt
     5881 catcatcgac caggtagcgg aacagtcgct gcgacaggac gatttggccg gaacgtttgg
     5941 ctatggtaat caccggataa cccggctgaa atatccaggt gtccatcaac gcgcgcaccg
     6001 gctgatgcgt ggaatcttcg atggcgtccc ataaatcagt ggtctcggca ttggcatact
     6061 gatgccgttt taggtagagg cgaatgccgt cgcgaaacgg ctcggcgccc aggtactgtt
     6121 cgagcatgcg caaaaccgac gcgcccttct cataggtaag cacgtcgaac atgccggcag
     6181 cttcctccgg tttttccacc ttgaattcga tcggccgtgt gctcttgagg ccatcgatct
     6241 gcatcgcggc ggcgcgcgaa acggtaaaac tttcccagcg gcgccattcg ggtttccagg
     6301 catccaccgc caacatttcc atgaaagtgg cgaatgcttc attcaaccaa aggccgttcc
     6361 accacttcat ggtcaccaga tcgccaaacc acatgtgggc attctcgtgc gataccacgt
     6421 ccgcaactcg ctcgagctca ccgcgggttg ccttttccga gtcgaccagc aacgcggttt
     6481 cacgaaatgt gatcgcgccg agattttcca tcgctcccga tgcaaaatcg ggaatcgcga
     6541 tcaaatccaa tttatcgccg ggataaggga tgccgtaata ctctgcgaaa tgggttaatg
     6601 agaagctgcc gatctgctcg gcgtatttcg ccaaatgctt cttgcctggg accgcccaca
     6661 ccctaag
//
LOCUS       DASUVS010000473         9459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_209530, whole genome shotgun sequence.
ACCESSION   DASUVS010000473 DASUVS010000000
VERSION     DASUVS010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9459
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209530"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VF182_24835"
     CDS             complement(<1..902)
                     /locus_tag="VF182_24835"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HEX6440368.1"
                     /translation="MKTVIARDLQKNLKEWVGGLRQHQTLITRRGKPRGISVAINKGD
                     SIELMSIASPPATERDLRLDFFRGLALFCIFIDHIPNNILAKFTLQSLMFADAAEVFI
                     LISGYTAGMVYGRAMEREGFMISAVRIYHRVWQLYVAHVFLFMIFMATMAYTAGALNT
                     SLYAEEFGAANFLNEPGLAVVKALTLQFQPAFMDILPLYIVLLAVLPLVVTGFRSWPT
                     MVIFVSLAIWLAVQIDKRIALPAYPGPDQYWFFNPFAWQALFFLGAWFGWRANHGGVP
                     WLNRRWLFYLAVGLSLAGFLIRFS"
     gene            complement(1635..3011)
                     /locus_tag="VF182_24840"
     CDS             complement(1635..3011)
                     /locus_tag="VF182_24840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6440369.1"
                     /translation="MVISIAVAVVAALVILPRLGDQSLANWDEAIYGVLTRELLSDLG
                     LTLHYGGRPWFEKPPLAFWLMAASSSVFGLTEFALRLPSALFGIAAFVLQYLAGRRLA
                     NRTGGVLAVVLLLGVPQFIAYSRLAMTDVPLAALGMFSVVLLLYANGRSSLIMAAGAA
                     FGLAILTKSAAAFLFLPGLLAIAIAQHGLGFFRSREIWLAPTAAIAVALPWHVCLAWM
                     YGDSFVDQYFGLHILARFASPLEGHEGSSFYYLETYFQKAGFLAPVHAAGTALAVSLA
                     IVRRDRLLAAASILALAAFAIVSVQETKIGGYLTPVYPAAALAGALGIARVIQKPRPL
                     VALLAASTLLALPGIVIGRGPFAETLDLVHFSPEVRALRNTRLFANGRVPLLYSFGLS
                     EPALRFYLADDVQPIDEFELVRLFSKKQPFLCLTFNSEAAKFASKHPALGFEIIVSTE
                     SLALIGYP"
     gene            complement(3202..5175)
                     /locus_tag="VF182_24845"
     CDS             complement(3202..5175)
                     /locus_tag="VF182_24845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440370.1"
                     /translation="MIVRRWLVPSIGALLSGALMLACTAPRTIGSNRSPVICKELEEA
                     PDSRWKTITHDGVHWLVTPCGERFFSLGVNVMDGAVTQTASNDPSAYHWTRFYAALED
                     WAQTARQRVLAWGFNTAGGWSLDPTLLELPTTPNLELGRTARFHWFDPFDPATAERMR
                     AWAHRLVAPYKGNPYRIGYFIDNEIGWWNGALFIYYLKQPASNHTKQHLVARLREHYG
                     NDWKRFTADFVVPAGIASFEHLLQRTGRWPQLRPGGSGIHFIRRWTGIIAGLYYHLAH
                     DAIREADPEALILGDRLPIYYDPIAVRAMAPYVDVISTNYNVDSPDGWLARYFFAGLR
                     QLAPAKPILVSEWFFAAHENRSGNLNNGHLMTVPTQAERTDGAMAAAQRFAREPQLVG
                     LHWFQYHDHPRGGRDDGEDYNFGLVDINDRPYEQLVEAFSRVNPRLGGLHDQLPDPAQ
                     NNRDDPWQIPQAAIDARDRSLEEWPKERALLAPLITPRAEIPFGDFYVSWSPDGLHFA
                     MIAMDYYDPDLLAYGDEFPLQEAFRVDWGVDAGAGPRRFRLYIIPPRIFPESGTPKMR
                     PWLCDATPVSCKSVPDAVTTYFGSDQPRITVEVTLPWEALGLTEASSRENLRMELAAT
                     AWHRSRWMSWSGLPPLQAMQDPSQWRPVQLTGE"
     gene            complement(5172..5825)
                     /locus_tag="VF182_24850"
     CDS             complement(5172..5825)
                     /locus_tag="VF182_24850"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6440371.1"
                     /translation="MTERDLVDQKIRNFMEGLWARGDPWDLENSVYERDRCLQLVKMI
                     EGRRYHRVLEIGCGAGYLTRMLAAHADHIVALDISQTAIDRARSLSTSQTQVDFRTGN
                     VMEYKPQVDGPWDLIVFTETICYLGWLYPMFDVAWLAVQLFDATRSGGRLMLANSIDE
                     SEDWLLRPWIIRTYRDLFVNVGYTIESEKIHTATKRGIEFEVLMTLYCKPEEGKQDK"
     gene            complement(5846..6706)
                     /locus_tag="VF182_24855"
     CDS             complement(5846..6706)
                     /locus_tag="VF182_24855"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX6440372.1"
                     /translation="MQKLLPEHLKDFWQRAERHEHSTEDYNCEYERALAEYRQTWQRA
                     LILPGHDNLRESLVSELGSYTQCSDLTEVRVRCEQAVANLKREWANQVFSENRQAIEQ
                     FYDETPAMLYELMWWHALGDDLSPLAYVLALRFAQQHGCKSYLDFGAGVGAGGLLFAA
                     HGIQTTLADISSCLLRFSAWRLSLRKLPAQCLDLKHCHLPSESFDFITAMDVFEHLAD
                     PVETVEKLWNALKPRGFLYGRISADPDEDRPQHIVYDFEPTFDRMRALGFVRVWYDDW
                     LWGHQVFQKP"
     gene            complement(6765..7532)
                     /locus_tag="VF182_24860"
     CDS             complement(6765..7532)
                     /locus_tag="VF182_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEX6440373.1"
                     /translation="MLRESENHLRSEATAPAKYLIVNADDFGQSPGINRGIMTAHRDG
                     IVTSTSLMVRWPAAAEAATYAREHPSLSIGLHIDLGEQILRAGEWVPLYTVVPLESES
                     AVRTEISRQLELFDRLVGHAPTHLDSHQHVHLREPARPIVFELAQQLGIPLRCCSPEI
                     KYCGNFYGQAEDGTPLHDTISVDGLIRILERLPAGCTELACHPASVCDLQTLYSRERL
                     EELRVLCDPRVRAALTNMEIALRSFVEFALNRRRISD"
     gene            complement(7547..8713)
                     /locus_tag="VF182_24865"
     CDS             complement(7547..8713)
                     /locus_tag="VF182_24865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440374.1"
                     /translation="MQVGKSKVVVVLGIMGCLPVAGTGVAWNTIQHLIALRRLGYDVY
                     YVEATAVWPFNATTDDCTFPVTYISSLLNRFGFENKWAYVAAHSDNRCYGLSELQVND
                     LYARADAIVNLFGGTILRDEHMKCPIRIYLETDPVVHQLRIANGEQRYIDLVGAHTAL
                     FTWGANYGAPDCGVPVGSFTYKPTRSPVILDFWQSSYNPTTRYFTTIGHWDQSVKDLE
                     YNGERYSWSKHSEFLKILELPRRTPQELSLALAIDEPDAVQMLEGYGWHVEDAYQVSE
                     TLDSYRDYIWGSRGELTVAKDMNVRLRSGWFSERSACYLASGKPVVTQETGFSKSIPT
                     GVGLYAFQKLEDIPPAIAAINSDYRHHSQAAKEIAAEYFDAEKLLRQVMVDAGV"
     gene            complement(8829..>9459)
                     /locus_tag="VF182_24870"
     CDS             complement(8829..>9459)
                     /locus_tag="VF182_24870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX6440375.1"
                     /translation="PTRQPIVLSEWRPEKPVREVFTTVMNWTSYKPVVYGDQSYGQKD
                     LEFVRFQELPSLIAPSVVEIAVNAGKTRRTPRQLLARKGWRVVDPAEVCPDLESYRDY
                     IESSKAEWSVAKNGYVLGQPGWFSCRSACYLAAGRPVAVQDTGFGAVLPLGEGLLAFK
                     TLEEAVASIKEIETNYARHAKAAREIATEYFDATKVLTRLIEEAMNGRD"
BASE COUNT         2216 a         2658 c         2653 g         1932 t
ORIGIN      
        1 cagctaaacc ggattagaaa tccggccaac gagagaccca ccgctaggta gaacaaccag
       61 cgacggttaa gccagggaac tccaccatgg ttggcgcgcc agccgaacca ggcccccaag
      121 aaaaacagcg cctgccacgc aaacgggttg aaaaaccaat attggtccgg tcccggatat
      181 gccggcagcg caatccgttt atcgatctgc accgctagcc aaattgccag ggagacgaaa
      241 atcaccatgg ttggccaaga tcgaaatccg gtcaccacga gcggcaggac tgccaatagg
      301 acgatgtaga ggggcaggat gtccatgaac gcgggctgga actgtaacgt taaggccttc
      361 accaccgcta ggcccggttc gttgagaaaa ttggcggcgc cgaattcctc cgcgtagagc
      421 gaagtgttga gagcgccggc ggtgtacgcc attgtcgcca tgaatatcat gaacaaaaaa
      481 acgtgagcga catagagctg ccatactcgg tggtagatgc ggaccgcgga tatcataaag
      541 ccctcacgct ccatggcgcg accgtatacc atacctgcgg tatagccgga gatcaagata
      601 aacacttcgg ccgcgtcggc gaacatcagc gactggagcg taaatttcgc caggatgttg
      661 tttgggatat gatcgatgaa tatgcagaaa agggcgaggc cgcgaaaaaa atcaagccgg
      721 aggtcgcgtt cggtggccgg aggactcgca atactcatga gctctatact gtcaccttta
      781 ttgatggcca ccgagatgcc tctgggtttg cctcggcgtg tgatgagagt ctggtgctgt
      841 cgaaggccgc ctacccactc tttaaggttc ttctgtaaat cacgggcgat gacggtcttc
      901 ataaaatatc ttccgttctc tttcaaacag cgaaagtaac gagaatcgac gtcctgctta
      961 ggccgtaacg cgcgctctct tttacgccac actcatgctc gatcagcggc tccgcgagcc
     1021 atgtatgccg agccggacgc acgcgcctct gcgtcttgga ttccgatcct ttcaggccca
     1081 ttgagccgtg taaacagcgt cgatcggcaa atacatcgag tcgggccgat cttcacgatt
     1141 cgttcccatc gagtctcttc agaatcagtc cccttcggcg agcgcaatcg gctatatcat
     1201 ccgctgcttc atgctactac ttactatggt cgcagccaaa acagaattgt cgatgtaaca
     1261 acccgtgatc tcagcaagca aatactaggg gaagcggccg agtcatggtt gctcttggtt
     1321 gggatttcat cgatttatac ccgaatcgaa attcgcaaga attcaactaa cgaagcttgc
     1381 agattgagac gaaatctttt attggctcca gtgtctcagt tacgaattgc ccgcagcgaa
     1441 gtgggcgcct tcgtcgatct tttgcgagtc gctgtagatg accaagggat gcctgaacaa
     1501 tgaagcgaag caaaccatta gtattggtag aagaccggct gccgtgcggc atagcacgtt
     1561 aacgaaaacc ctgcggtcat gtcagattat tttgcctcac gacattggtt tcgtttcaga
     1621 aatattggcg ctcctcaggg atagcctatg agcgcgagag attccgtaga gacaatgatt
     1681 tcgaacccca aagccgggtg cttactcgca aacttggctg cctcagaatt gaacgtcagg
     1741 cagagaaaag gttgcttttt ggaaaatagc cgaaccagtt cgaactcgtc aatcggttgg
     1801 acgtcgtcag cgagatagaa gcgcaaggca ggttccgaca gcccaaacga ataaagcagg
     1861 ggaacacggc cattggcgaa cagtcgagta tttctgaggg cgcgaacttc cgggctgaaa
     1921 tgcacaaggt ccaaggtttc agcgaacgga ccgcggccaa tcacgatccc gggtagcgca
     1981 aggagagtac tggccgcgag cagagcgacc agtggccggg gtttctggat tactcgagcg
     2041 atcccgagag cccctgcaag agctgcagcg ggataaaccg gcgtaaggta cccgccgatt
     2101 ttggtctctt gcacgctcac gatggcgaag gcagctaacg ccagtatgct ggcggcggcg
     2161 agcaagcgat cgcgcctgac gattgcgaga cttacggcaa gcgctgttcc agccgcatga
     2221 accggagcga ggaacccggc cttctgaaaa taggtctcca gataataaaa agaactcccc
     2281 tcgtggcctt cgagcggcga agcgaaacga gccaaaatat ggagaccgaa gtattgatca
     2341 acgaacgaat caccgtacat ccatgccaag cagacgtgcc agggcagcgc aacagctatg
     2401 gccgcagttg gcgcgagcca gatctcccgc gaccgaaaaa aacccagacc gtgttgtgca
     2461 attgcgattg ccagcagccc cgggagaaag agaaaggcgg cggcggattt agtgagaatg
     2521 gcgaggccga aagctgcgcc ggctgccatg atcaaagagg atcggccgtt agcatacaat
     2581 aggagaacaa ccgaaaacat tcccaaggcc gcaagaggaa cgtcggtcat cgccaggcgg
     2641 ctatatgcga tgaactgagg gacaccaagg agtaagacca cggccaacac gcctcccgtg
     2701 cgattggcta agcgccgccc ggccaagtac tgaagcacga aagcagctat tccaaagagt
     2761 gcgctcggca ggcgcaaggc aaactctgtc aggccaaaga ctgaagagct tgcagccata
     2821 agccagaatg ccaaaggcgg tttctcgaac cagggccggc cgccatagtg caaggttaga
     2881 cctaaatcag agagtagttc gcgagtcagg accccgtaga tcgcttcatc ccaattggca
     2941 aggctttgat cgcctagtcg gggaaggatc actagcgcgg caacaacggc aacggcaatg
     3001 gatataacca agtcgcaatg atccaaaaca cttgattgta gctgggagtg gtcggccgcg
     3061 gatctcatgt tcgcggcttt aaatgctgtc tggtgtatca tccgcttcaa cgggttgaaa
     3121 tctaacaatc aggtttgaat cggtgtagag acgaccggcc gcgtggttag tcgggtgttt
     3181 ccacgcccgt aatcctcgat ctcactcgcc tgtcaattgt acaggacgcc attgcgaggg
     3241 atcttgcatt gcctgaaggg gcggaagtcc gctccacgac atccagcggg agcgatgcca
     3301 tgcggtggca gcaagctcca tccgtagatt ttcccgggaa ctcgcctcag tcaagcccag
     3361 agcttcccag ggaagcgtca cctcaacggt gatacgcggc tgatcgcttc cgaagtaggt
     3421 tgtcacggcg tcgggcacag acttgcacga gaccggagtc gcgtcgcaca gccagggccg
     3481 cattttcggc gtgccgcttt cgggaaaaat gcgtggcggg ataatataga gcctgaaacg
     3541 ccggggccca gcccctgcat ccacgcccca atccacgcgg aacgcttcct gcagcgggaa
     3601 ttcatcgcca taggcgagca gatcgggatc ataatagtcc atagcgatca tggcgaaatg
     3661 cagaccatcg ggactccatg acacatagaa atcaccgaag ggaatttcgg ctctaggggt
     3721 gatcagcggc gccagcagcg cccgctcctt cggccattct tcgagagagc gatcgcgcgc
     3781 atcgatggcc gcttgcggaa tctgccaagg gtcgtcgcga ttattctgag cggggtcggg
     3841 aagctggtca tggaggccgc cgagacgagg gttgacccga ctgaacgctt cgacgagttg
     3901 ctcataggga cgatcattta tgtccactaa tccaaagtta taatcctcgc cgtcatcacg
     3961 acctccccgt ggatgatcat gatattgaaa ccagtggagc ccgacgagct ggggctcgcg
     4021 ggcgaatcgc tgggccgccg ccatggcgcc atcagtgcgc tcagcttgcg tcggcacggt
     4081 catcaaatgg ccgttattaa gattgcctga gcgattttcg tgcgccgcga aaaaccattc
     4141 ggaaactagt atgggcttgg ccggggcgag ctgtcgcagc ccagcaaaaa aataccgggc
     4201 gagccatcca tcagggctat cgacgttgta gttggtggat atgacgtcga catacggcgc
     4261 cattgctcgc accgcgatgg gatcgtaata gatcggaagg cgatcgccca gtatcagggc
     4321 ctcaggatcg gcctcacgaa tagcgtcatg agctagatga taatagagtc cagcgatgat
     4381 ccccgtccaa cgccggataa aatggattcc cgaaccaccg ggtcgcaatt gcggccagcg
     4441 acccgtccgt tggagcaaat gctcaaaaga ggcgatccct gcagggacga cgaaatccgc
     4501 cgtgaaccgt ttccagtcat tgccgtaatg ctcgcgcagc cgagcgacaa gatgctgctt
     4561 ggtgtgattg ctggcaggct gcttcagata atatataaac agggcaccat tccaccagcc
     4621 gatctcattg tcgataaaat agccgatgcg gtacggattg cctttatagg gagcaaccag
     4681 gcggtgtgcc cacgcacgca tgcgctcggc agtggccggg tcgaacgggt caaaccaatg
     4741 aaagcgggcg gttcgcccca attcgagatt gggagtggtc ggtagctcca ataacgtcgg
     4801 atccagggac cacccaccgg cagtgttaaa gccccacgcc aagactcttt gacgtgccgt
     4861 ttgcgcccaa tcttccaatg cagcatagaa tcgagtccaa tggtaggcgc tggggtcgtt
     4921 ggaagcggtt tgcgtaaccg cgccgtccat gacattgacg ccgagcgaaa agaagcgctc
     4981 gccgcatggt gtcaccaacc aatgcacgcc atcgtgggtg atggtcttcc agcgcgaatc
     5041 aggcgcctct tccagctctt tgcaaatcac cggcgagcgg ttcgaaccga tcgtccgagg
     5101 ggctgtgcag gccagcatca gagcgcccga aagcagtgcg ccaatgctcg gcactagcca
     5161 gcgacgaact atcacttgtc ctgctttccc tcctcaggct tgcaatagag cgtcatgagt
     5221 acttcgaact caatgccgcg cttggtcgca gtatggattt tctcgctctc gatcgtgtag
     5281 cccacgttga caaatagatc tcggtacgtg cggatgatcc acggacggag gagccagtcc
     5341 tcactctcat cgatactatt cgcgagcatc aagcggccgc cgctgcgtgt cgcgtcaaaa
     5401 agctgcaccg caagccaagc cacgtcgaac atcggataga gccagcccag gtaacatatc
     5461 gtttcggtaa acacgatgag atcccagggt ccatccacct gcggcttgta ctccatgacg
     5521 ttacccgtgc ggaaatcgac ctgcgtttga ctcgtgctca gtgaacgcgc gcggtcaatc
     5581 gccgtttgcg agatgtcgag cgcaacaatg tggtccgcgt gagcagctag catacgcgtg
     5641 agatagccgg cgccgcatcc gatctcgagc acgcgatggt aacgccggcc ctcgatcatt
     5701 ttgaccagct gtaaacatcg atcccgctca tacaccgagt tttccaaatc ccatggatca
     5761 ccgcgtgccc aaagaccctc catgaaattg cgtatcttct gatcgacaag atcgcgctcc
     5821 gtcatagcgt cattactcct gtgcctcacg gtttttgaaa gacctgatgg ccccaaagcc
     5881 aatcatcgta ccagaccctg acaaagccaa gggcgcgcat gcggtcaaag gtgggctcga
     5941 aatcatatac gatgtgctga ggccgatcct cgtcagggtc tgccgagatc cgcccataga
     6001 gaaatccgcg tggtttgagg gcattccaga gcttctcgac cgtttccacc gggtcggcca
     6061 aatgttcgaa gacatccata gcggtaataa aatcgaacga ctcgcttgga aggtggcaat
     6121 gcttaagatc gaggcattgg gcaggcaatt tcctgagact aagtcgccag gcgctaaagc
     6181 gcagcaaaca ggagctaata tccgccagcg tggtttgtat cccgtgagcc gcgaagagaa
     6241 gaccgccggc tcctacaccc gcaccgaagt cgaggtaact cttgcagcca tgctgctggg
     6301 caaagcgcaa cgctagaaca taggcgaggg gcgaaagatc gtcgccgagg gcatgccacc
     6361 acatgagctc gtaaagcatg gccggagtct catcataaaa ctgttcgatg gcctgccgat
     6421 tctctgagaa gacttgattc gcccactcac gcttgaggtt ggccacagcc tgctcgcagc
     6481 gaacccggac ctcggtcaag tcggagcatt gcgtgtagct gccgagctcg gacacaagac
     6541 tctcacgtag attgtcgtgt cccggcaaga tcagggcgcg ctgccaagtt tgccggtatt
     6601 cggcaagcgc gcgttcgtat tcacagttgt aatcttcggt ggaatgctcg tgccgctcgg
     6661 ctctctgcca aaagtccttt aagtgctcgg gtaataattt ttgcatgatt ccttctgctt
     6721 cgctcgcatc atccgctggc tcaggaaatc ctaagggccg cgcgctagtc ggagatccgc
     6781 cggcgattca gcgcaaactc gacaaacgaa cggagagcga tttccatgtt cgttagcgcg
     6841 gctcgcactc gcggatcaca taacaccctc agttcctcga gacgttcccg agagtacaac
     6901 gtctgcaggt cgcagacgct cgcgggatga caggccagtt ccgtgcaacc cgccggcaac
     6961 ctttccaaaa tacgaatcag gccatcgacg ctgatcgtgt cgtgtaaggg tgtaccgtcc
     7021 tctgcttgcc catagaagtt gccacaatac ttgatctccg gcgagcaaca tcggagcgga
     7081 attccgagtt gctgagcaag ctcaaaaaca atggggcggg cgggctcgcg gagatgaacg
     7141 tgctggtgcg aatcgagatg agtgggagca tggcctacca ggcgatcgaa cagctcaagt
     7201 tggcgggaga tttcagttcg caccgcgctc tcactttcca gcgggacgac ggtgtagagg
     7261 ggcacccatt ctcccgcgcg taagatctgc tcgccgagat cgatatgcaa tccgatgctt
     7321 aggctcggat gctctcgggc ataagtcgcc gcctcggcgg cggccggcca gcggaccatg
     7381 agactggtac tggtgacaat gccgtcgcgg tgggcggtca tgatgccacg gttgatcccg
     7441 ggactctgcc caaagtcatc cgcattgacg atcaagtatt tggccggagc agttgcctcg
     7501 gagcgtaggt gattttcaga ttctctcagc atagcatcgc ttccgtctaa acgcctgcgt
     7561 cgaccatcac ttgcctcagc agtttttcgg cgtcgaagta ttccgcggca atttctttgg
     7621 cagcttggga atgatggcgg taatcgctgt tgatcgccgc aatggcaggt ggaatatcct
     7681 cgagtttctg aaatgcatac aggccgaccc cggtcggaat ggacttgctg aaacccgtct
     7741 cctgggtcac cactggcttg ccgctggcga gataacaggc gctccgttcg ctgaaccaac
     7801 cgctgcgcaa acgcacgttc atatctttgg cgactgtaag ctcaccgcgg gagccccaaa
     7861 tgtaatcgcg gtagctgtcc agggtctcgg aaacttgata ggcgtcttcg acatgccagc
     7921 cgtatccttc gagcatctgc acggcatcgg gttcatcgat tgccaaggcc aacgaaagct
     7981 cctgaggggt gcggcggggc agttcgagaa ttttcaaaaa ctcggagtgc ttgctccatg
     8041 aatagcgttc gccgttgtat tccagatctt tgaccgactg atcccaatgg ccaatggtcg
     8101 tgaagtaccg cgtcgtcgga ttatacgagg attgccaaaa atcgaggatc acaggggacc
     8161 gggtcggctt ataagtaaaa gatcccaccg ggacaccgca gtccggcgcc ccgtaattcg
     8221 ctccccaagt gaatagggcg gtgtgtgctc ctacgagatc gatgtacctt tgttcaccgt
     8281 tggcgatccg gagttgatgc actaccggat cggtttcaag atagatccgg atcggacatt
     8341 tcatgtgctc gtcgcgcagg atcgtccctc cgaacagatt gacgatggcg tcggcgcggg
     8401 cgtagagatc gtttacctgc agctctgata acccgtagca gcggttgtcg ctatgagcgg
     8461 caacataggc ccacttattt tcaaagccga agcggttcag cagactgcta atataggtca
     8521 ccggaaacgt gcagtcgtcg gtggtggcat taaacggcca tacagcagtc gcttccacat
     8581 agtagacatc gtatcccagc cgccgtagtg ctattagatg ctggatcgta ttccacgcta
     8641 cgccggttcc cgccacgggt agacagccca taatccctag cactacgacg acctttgatt
     8701 tgcccacctg catgcggctt ctcttcctat caatgctttg gaaaaaacgt tatccctggc
     8761 gatcgggcgt tcatgcgatc cagtggtcag cgtctcggac gagagaccgg cgctttgtga
     8821 gggaacggtc agtcgcgacc gttcatcgcc tcctctatga gacgcgtgag cactttcgtc
     8881 gcatcgaaat actccgtcgc gatctcacgg gcagccttgg catgccgcgc ataattcgtc
     8941 tcgatttctt taatcgacgc caccgcttcc tccagagtct tgaacgcgag aaggccttca
     9001 cctaggggaa gtaccgcgcc aaatcccgtg tcctggacgg ccaccggcct tcccgccgct
     9061 aaatagcacg ccgatcggca actgaaccaa ccaggctgcc ctagcacgta gccgttcttg
     9121 gcgacgctcc actcggcttt ggaagattcg atgtaatcgc gataactttc gagatccgga
     9181 cacacctcgg ccgggtcgac cacgcgccat cccttgcggg ctaacaattg acgtggagtc
     9241 cggcgagttt tcccggcatt gacggcgatc tccacaactg atggggcgat gagactcggt
     9301 aactcctgga agcgcacgaa ctccagatct ttttgtccgt agctttgatc gccgtacacc
     9361 acaggcttgt aactcgtcca attcatcacc gtggtgaaaa cctcgcgaac cggtttctca
     9421 ggacgccact ctgacagcac gattggctgc cgcgtgggg
//
LOCUS       DASUVS010000474        22772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_430475, whole genome shotgun sequence.
ACCESSION   DASUVS010000474 DASUVS010000000
VERSION     DASUVS010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 22772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22772
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430475"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..600)
                     /locus_tag="VF182_24875"
     CDS             complement(253..600)
                     /locus_tag="VF182_24875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015954778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6440376.1"
                     /translation="MAETSITKVNAKSSPKGSMGQKYLAAGVSMAMRLWEDEQPGEEK
                     PASERDYEAVGYVIKGRAELHLGPQLVLLEAGDSWVVPRGASHSYRILEAFTAIEATH
                     PPAFVHGRDEKKL"
     gene            822..1460
                     /locus_tag="VF182_24880"
     CDS             822..1460
                     /locus_tag="VF182_24880"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HEX6440377.1"
                     /translation="MAVLHSEVVPKNSGRAFIVKKGQRVRIAGKSIVDFVAFNLDDSS
                     ERFDQARTKTNQVKIFISTGDILFSKRNNPMLTVVEDTFTEGRHDLQKGMCSRKRFEM
                     VAQGKAKRVFAEGVDINPKAPDDIPDHGCFENLSDALRQWKIAPEDIPSPFNIFQCMR
                     IDPETGIMYDTMIRPKDEAHVDFRAEMDCLVAASACPESGRGQAIRVEIYDQ"
     gene            complement(1621..2442)
                     /locus_tag="VF182_24885"
     CDS             complement(1621..2442)
                     /locus_tag="VF182_24885"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HEX6440378.1"
                     /translation="MVIRELKDGRSYVSLQEDHAELAAQFAAHWGNERFSQLRPYKTM
                     VFATTYHDSGYREFEGNPPINVEKGRPYAHRENIPSFEAVELEAYAKNVDWLCSHDLY
                     AGLIVSMHRTGLWHNRYNVFTKPAGNVRERSQEVQNAKKELEAKQEQYKKTLAAENPA
                     FEDELWHNYRTLQIFDLLSLYFCCDGYASEKEFKEYTIAPIRIAYDSNEEAELKITPS
                     GPGSVKMTPYPFDESPMQFSVRTRLLTPAKGQSAEAGLEAYQKAPRQLLTFEISN"
     gene            complement(2730..3185)
                     /locus_tag="VF182_24890"
     CDS             complement(2730..3185)
                     /locus_tag="VF182_24890"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX6440379.1"
                     /translation="MQQTIFFDKAQIGDSIPPREHGPLTIVDTVRWAGLQENVQHLHF
                     DRDHVRQHNGLPTFIASGAYRQALLIRMLTDWIGPRGWLRKINVRHTYSTFEGDRMRF
                     FARITEKSSDSSDPWISCELEGKNQEDRQILTGHCTLILPLRSEDGRIT"
     gene            complement(3203..4318)
                     /locus_tag="VF182_24895"
     CDS             complement(3203..4318)
                     /locus_tag="VF182_24895"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6440380.1"
                     /translation="MTRIIDLTGIIGSYATRLFVEEGHEVIRVEETNGDDLRRLPPFL
                     ADHKDLEHSAYHQFLNAGKKSLTLDFDSPTGRKLFIDLLGRSDALVAERLPIEEQLCF
                     EANPKLVVTKIENDSPEICAMARSGLLSLTGQPNQAPMILGAHIPYLAVGAYVGVATA
                     AALLAARNCGRGIAATVSVRQSLESFAEQAMVEYSFSGTITERRGSKGAITAISGALP
                     CKNGHWVISQIHRPGRWDKFLDWIQDPDLSSNPSLAQEENQHKHRDFIMDRLHAWATR
                     FTKEELVEEAQRRHFPASPVATTLDLIDDPQLVARGFLREMNHPLLGQIIFPHGAIAT
                     VTGVQLAPAPRLGQHNNEILGELGNAPPPHENFNTGA"
     gene            complement(4319..4864)
                     /locus_tag="VF182_24900"
     CDS             complement(4319..4864)
                     /locus_tag="VF182_24900"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6440381.1"
                     /translation="MSQNSPSIITPELRAAIGRQSPARPLEVLSASDVRRYVDATGDA
                     NRLWLDDEFARAAGYRGRLLPPTLVGWVPFSFRESTERANADPMDLRRQLPLPPGYTN
                     VRNAGSETEWIAPVYLDEQLFTRSCIVDIVAREGRAGLGIYITQEEQVLNPDEQIVLR
                     RRHTVVIFPDKTYSDRADEHK"
     gene            complement(4861..6084)
                     /locus_tag="VF182_24905"
     CDS             complement(4861..6084)
                     /locus_tag="VF182_24905"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6440382.1"
                     /translation="MSDNAGGAKYPAVPAPLDKIRVLDFTHVLAGPFCTRLLADLGAD
                     VVRVESSKHPDHPWQSTYISDDQRHASYLNTNRNKRSIAIDLKNETGQKIALKLAAVA
                     DVVIENFSAGVMSRLKLDFATIEKLNPRIIYVSMSGYGHNGPRRDWTSMNMNLQGYSG
                     LMTVTGTEGDPPTAISNSWNDYIGGLHACFAILQAIGKRAGSGTHIDLSQFECSASMI
                     GPLLLSSAVNGKVPHRLGNRSDILAPQGVYRCTGADNWCALSIENDEQWEALAKVLGN
                     EKLRSDTRLSSAAGRMDHHDEIDRQIEAWTEQYSSSEVERRLKEAGIPAERVRRIDQV
                     IDEPDPAKVFSRMQERRIGSMLTTRLPFTLSPVSLPSPSTAPSLGEHNTDVMRRWLNL
                     SGEQFDEIETHKAFT"
     gene            complement(6074..7042)
                     /locus_tag="VF182_24910"
     CDS             complement(6074..7042)
                     /locus_tag="VF182_24910"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440383.1"
                     /translation="MTIKNATRLGLTLFALIVWPQIPARSQERVRIGISAASLGFLPT
                     VLAERKGFYSKHGINSEHVLVPCAIATNALLSGDLDSAVCTGPGIAGAIKGLPIKLVM
                     TTQDKLGYLLLVKPEVQKLTDLRGKTIGISTFGSQLYLTSVTLFRQAGMEPGKDVNLL
                     PGGDNTARLAAMDGGKIDAAFVSSPADIFGAKRGYKVLLWTRDHVPLTQNAMVVTDKS
                     LKQSPEQVKRTIKGTIEALRFIRDHQEESVAIASKWLKLDLATTRAAFDNYLPCYSVD
                     GGLSDQALRDLVRYELDRANLKKDIPVSQVASRDLLQQAQKELNVR"
     gene            complement(7039..8040)
                     /locus_tag="VF182_24915"
     CDS             complement(7039..8040)
                     /locus_tag="VF182_24915"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440384.1"
                     /translation="MFSRTAILLLALSFAGLGQAQERTLQPLVVSYSSFTGNRAPLWI
                     AKDLGLYEKYGLDVKLVSIAAGSVSLTALLAGNVHLTTDSSSGVVGAVARGAPIVTVS
                     TNGTLPYQLIAVPSIKSTAELKGKIVGSSRIGAGTDFLLRRILAKLSFIPGKDVSLVP
                     TGVSESDKRIQLMFQGKIDATIGESDKVFQFTELSGQKISVLGEPKDFGVPSPGSEIN
                     TTRQILKDNRPRLKAFFMAYSEAIAAGRKNKELAYQVFRKYMRVDNPKLLEFTYRVQF
                     LEALPGKPYPREDAIQASIEDLRESTPKLDGMKAAEFFDPSLIREIDQEGFFTRTDK"
     gene            8268..9125
                     /locus_tag="VF182_24920"
     CDS             8268..9125
                     /locus_tag="VF182_24920"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6440385.1"
                     /translation="MELQQHHSRGNVFWPGNNRIAVFLSFDFQGGEDVRPDKNGRVNH
                     EEYTQAEYGPNTGIWRILRILSAHDVKATFLTCGGIAERYPEALAAIVAQGHEVAGHG
                     YHHEVARDLSKDEEREVMKRTSEMLLNRAQKPPVGWRSCTQSPNSLELLMEHGYLWNS
                     NSFSFDLPFVWRNGDKQLVELPRQPFGDGRTYGHRDSGNPNDTLTVWKAMFDQFYEES
                     KIAPTFCPFQFHPYISSRPGRAKTLSDLIQYMKSHEGVWFATGSEVAKWWLDNGFSAE
                     AAAQVSKVA"
     gene            complement(9218..9769)
                     /locus_tag="VF182_24925"
     CDS             complement(9218..9769)
                     /locus_tag="VF182_24925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440386.1"
                     /translation="MIVAMPYGCAGSRNLSQASRIGLEKAIDWWQKSMQTSKHKTYFI
                     VAGYTDDGGEELSARREVIDKAGVDPELLNNLIEISARNEETLALKISRVRQLLPIET
                     LTVFVETRNAVSVRTIFKRKFGKTLQIRKFKARFEFNHQWITTSTSFAWFSRNWLLRV
                     WFAVKKRMGRRLRKRVRFLFRSY"
     gene            9777..9989
                     /locus_tag="VF182_24930"
     CDS             9777..9989
                     /locus_tag="VF182_24930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440387.1"
                     /translation="MVVVSFSVSLAAGKGQDRQIVTVTGLPPDREIERAFSVTSRSIC
                     FEQTCLAILPVIHLFLSADLNVAVMR"
     gene            complement(10074..11120)
                     /locus_tag="VF182_24935"
     CDS             complement(10074..11120)
                     /locus_tag="VF182_24935"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440388.1"
                     /translation="MDRENRINFLVYLRYLFGRIWLIIIFLSLSTSSGAEPLRIAYTS
                     IAVVYGPLWITQEAGIFKKHGIDPEFIYIAGGPPSLQALIAGDVAISFTAAGATVAAN
                     LSGSDVVLLGASIDSLPFELWSIPRIKQPAQLKGTKLGVSRIGATTDFVARYLLKKWG
                     MQADKDVGIFQAGSGPQVFAALKSGSVQSGVLSIGPETLRAESEGYTRLADVPSELVY
                     PFGPFAARQSFLKKEPELITRFMKAYVEGIHRFKTDKRTALATLEKYTKQKTTPAVEK
                     IYQVYATKHFKRVPEATQAGIQTILDELAATRPIPPGIEPDRFVESKFVRELASSGFV
                     DNLYKERQAARPPP"
     gene            11327..11716
                     /locus_tag="VF182_24940"
     CDS             11327..11716
                     /locus_tag="VF182_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020411785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440389.1"
                     /translation="MKKAIVIGFSVLALGSAVTASADDRSYGWRGDRINQRLDARGAR
                     INQHLDARGDRINDKLDARAARARADGKFKLAERLDRQGDRIERHLDKKGDRIEAYWD
                     RRGDRIDRRFDRRDHRDGRFAHNGYRR"
     gene            complement(11800..13065)
                     /locus_tag="VF182_24945"
     CDS             complement(11800..13065)
                     /locus_tag="VF182_24945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440390.1"
                     /translation="MSQDAMNRLVAALTHGAGLRDDFIYYMTFDDYKTLPDPFARTAF
                     TVEEVQQTIYRYANPEKPGELFRWDIHGRLFTPAKASLGPIGVVMIHGGAANEYEFIF
                     TPDGPEEYPDLTRIDPAMARVGVAQHIASLGIPVLALSLPGHYSRKPWLPIGERRPEF
                     VIGETPGDAELRNRLAVYTFRMCTEAIKALVERSLPGQDIFMWGHSTGGEYFYLMEQY
                     GLKNNLIGGLGFGTGMPAWVRKEWDLACAEKSAEERAAPFRNLTDLSRRSPGGYIKSG
                     YVGPNQPWGSAEKWFELENHRRPQFKPFLQDIEHSAHDILLPEIRRISGLPDEELFIT
                     YKSDLTCLRGKKMLHIVGDYDKGHWVEGGKKGLEFRREVYAFNRFAPHTKALRLLHVP
                     NLTHYGHVENYNERLANIMVTGFKEYFSG"
     gene            13239..14333
                     /locus_tag="VF182_24950"
     CDS             13239..14333
                     /locus_tag="VF182_24950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6440391.1"
                     /translation="MSGDSQKASYAKVDRKAEVMDTPYDRWIASQGIDVIRGFFVENL
                     YTIPLKWWDRLGGNGVYIMLDGTGYLDDAYVCGIPPGKSLNPQRHIYEALIYVLDGRG
                     ATTVWQTNGSKQSFEWQTGSLFAIPLNAWYQHFNGQGDRDARLLAVTTAPLVFNLFHC
                     EDFVINNPYSFTDRFKGEEGFFGGKGTLYSDRVVETNFVADTINIEPVAWTERGKGNA
                     TIFFEMSDSMMGSHVSRFPVGIYKKAHRHGPGAHVFILTGKGYSLLWPEGKEMTRVDW
                     KPGSIVVPPEGWFHQHFNSSAEPARYLALKILSRKYKLQPGKIQSDVPLAFGGWQIEY
                     EDEDPMIRKIFEEECAQSGAEVRMPAIARD"
     gene            14404..15231
                     /locus_tag="VF182_24955"
     CDS             14404..15231
                     /locus_tag="VF182_24955"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6440392.1"
                     /translation="MLKPIGLTEGHYECRSLDETLPIFTDLLALEVVERQDHGAVLKH
                     PNTAWRLVVHEGGPNAVNKPHTNHYGFRVSSSREIPKAYEYLTDHKDQYKIKSVTKPH
                     EAHFAYSVYFKEPGGNDLEIEYYNPAAVKQGRKIAAPHWTEPLPEEKFPGRGYVPQAM
                     THGTLNCDDKEVNDRFVKEVLGLEIIGGGRTSTYISHPSTPWYVVVLPTTRREYLTAA
                     NRFTLKLASAEEVADAHKEFTTKGQAIGINELWDLQENGRTHFIFSDISKNWWEVTS"
     gene            complement(15274..16089)
                     /locus_tag="VF182_24960"
     CDS             complement(15274..16089)
                     /locus_tag="VF182_24960"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440393.1"
                     /translation="MSIVFACAASHAPGMTAWTEAAPQEQRDKILGSYHKLGEMLAAS
                     KPDALVIVSVEHWANFFLNQMPTFCIGRASYYDGPVEEWLKIPKTRVPGDSALGVQLL
                     DACLDSGFDPAFSDELLFDHATILPLHFLNPGMTVPVVPVIINTLTTPMPAPKRCFEL
                     GRVLGKILEEHPKRIALVATGGLSHWPGEAKMGKINIAFDEQFLKTLINGRRARLAEY
                     SHSEIDKEAGSGGHEIRTWITVAGAVGERRATLLAYEPVHPWATGCGLVAFTG"
     gene            complement(16093..16347)
                     /locus_tag="VF182_24965"
     CDS             complement(16093..16347)
                     /locus_tag="VF182_24965"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440394.1"
                     /translation="MSLYQLQKLIYDVNRNPERRDAYRADNAGFLARYELTAEEQAAI
                     ANLDVRKLYTCGVHPLLLRPFTLLHRVSNEDYARALAGLE"
     gene            complement(16349..17443)
                     /locus_tag="VF182_24970"
     CDS             complement(16349..17443)
                     /locus_tag="VF182_24970"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6440395.1"
                     /translation="MNQPRSLEQIKNDIRARLGQRAPFLHADKAEAEEALGKMSSVQG
                     ETWAQAWNELGARWEEKARSAESAGNKQEAKESYLKSYGYYGIARHPFPSTPGKHHGY
                     VKTREMFLAASKYFEIPIERVSIPFHDKQIVGHLRLPKRVPAPMIMHWGGIDNWKEER
                     HSFAESFVKQGWGCFVMDSPGTGESPVLAGPDAHQVHTTALEYLRKRPEVDTTRIAVV
                     GASFGGHWSTKLAHLAPDKLRAAVNWGGGIHYFFQPQWQERSRNADSYLFDLIEARAH
                     LFGMNSFDELCRVMPALSLLDQGWLDKPCAPLLIVNGKEDKQVPLEDFYLLLEHGDPK
                     TIRLFPGGHMGAIPNIFQTVITWLHQRLDG"
     gene            17800..18660
                     /gene="folD"
                     /locus_tag="VF182_24975"
     CDS             17800..18660
                     /gene="folD"
                     /locus_tag="VF182_24975"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX6440396.1"
                     /translation="MASILDGKAVAKEVQKKIKEEVDGLERRWGMAPGLAVVFVGDDP
                     ASHIYVKNKEKACNEVGIMSFEHLLPATVSEKELLTLVHSLNKNKDVHGILVQLPLPA
                     HIRSERILQAVSPFKDVDGFHPMSQGLLVIGADGFRPCTPLGVMKLLEAADCALKGKN
                     AVVVGRSNIVGKPVALMLLEKHATVTICHSRTASLRAEVARADVLVVAIGKAGLVRGD
                     WIKPGAVVIDVGVNRLPSGKLTGDVEFEAAKERASAITPVPGGVGPMTICMLLHNTLK
                     AAKDSIQRER"
     gene            18689..19777
                     /gene="gcvT"
                     /locus_tag="VF182_24980"
     CDS             18689..19777
                     /gene="gcvT"
                     /locus_tag="VF182_24980"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX6440397.1"
                     /translation="MRPMNRTPLFAAHRRAGGRMVEFAGWEMPIQYSGIIAEHMAVRS
                     RAGIFDVSHMGEIAVRGAGAEAMCQRLSANDVARMQVSQAQYNLFLNDRGGVIDDVIL
                     YKRQPDDFLICVNASNSEKDFQWLRQHVTGEIQVENVSADYAQLALQGPLAEEILQPL
                     TAMALAEIQSFYFAESEVAGTRCLIARTGYTGEAGFELYCTPRDAEKLWQGLLDAGSP
                     AGLVPAGLGARDTLRLEKAYPLYGHELDDSTTPLEAGLQWLVKFSKGSFIGCEVLLRQ
                     KQAGVKRKLVGLELIEPGIARTDYRLLKDGRCIGRVTSGTRSPTLGQSIALGYVEAKE
                     ARAENILEVEIRNKPVRARIVPLPFYGR"
     gene            19809..20246
                     /gene="gcvH"
                     /locus_tag="VF182_24985"
     CDS             19809..20246
                     /gene="gcvH"
                     /locus_tag="VF182_24985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX6440398.1"
                     /translation="MEIPEGLRYSKEHEWVLVEGKTATIGITEYAQEELGDIVFVELP
                     EVGEKIVKDDPFGAVESVKAVSDIYAPVSGTVLEINDSLPDSPETINDDAYGDGWMIR
                     VEMQDMDDLKDLMTAEEYAEYIEQQKEEDDEDDETEDDDEEEE"
     gene            20256..21602
                     /gene="gcvPA"
                     /locus_tag="VF182_24990"
     CDS             20256..21602
                     /gene="gcvPA"
                     /locus_tag="VF182_24990"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX6440399.1"
                     /translation="MSYTPHTEGDKNQMLDAIGARGIEDLFRHIPEKFRSTAKIDLPD
                     GISELAVRQRLAALAAKNAAANDWELYLGGGIYHHFIPSAVDAIISRSEFSTSYTPYQ
                     PEVSQGTLQVLFEYQTLICQLTGMEVSNAGVYDGASATAEAILMARRIQPPTRRKVLV
                     SRALHPQYRAVIATYLRNLSGLTLEEIPFDGTGATDIDQVARQLNERTMCVLVGYPNF
                     FGVIENLAPLHELCRAAGAQLITATTEPLAFGVIKPPGAFGVDIAVGEGQSLGVPMAL
                     GGPGFGFFASQRKFMRNLPGRLVGETVDSEGRRGYVLTLATREQHIRREKATSNICTS
                     QTLCTTAATVYMAFLGRSGMRRLAEVNVARAHETHRRIIEGGKFEGCFPAPFFNEFVV
                     RGKQLDEIMHRCLARKIVPGIPLARWYPELPDCLLVCVTEMNDREGIDRLVRAISN"
     gene            21580..>22772
                     /gene="gcvPB"
                     /locus_tag="VF182_24995"
     CDS             21580..>22772
                     /gene="gcvPB"
                     /locus_tag="VF182_24995"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX6440400.1"
                     /translation="MSEPSRTDPRQPNQFSRLLFESGSRGRTGFSWTEKTPAVSDSIP
                     EAYLRRDISDFPELGELELLRHFTRLSQCNYAIESHFYPLGSCTMKYNPKINEVVARY
                     PGFSLIHPLAPPALNQGALELLFALERMLGEISGMDDVSLQPSAGAQGELTGLMLIRA
                     YLSAQGKPRKKIIVPDTAHGTNPASSTLCGYDVVQISSNEHGVIAAAAVADAMDDEVA
                     ALMITNPNTLGIFEKNIEAVADVVHGKGGLVYLDGANLNALMGIAKPGHMGVDVLHMN
                     LHKTFSTPHGGGGPGAGPVAVKTLLRDFLPTPRIVKTTDGYDLIEDMPASIGRVRSFF
                     GNFGVLVRAYAYILSLGANGLEETSRMALLNANYIRSKLEGHYQLAYNEPCMHECVFN
                     DRNQH"
BASE COUNT         5151 a         6053 c         6338 g         5230 t
ORIGIN      
        1 attgaagctc actgatttaa gagaaagctt gcaagtccgc gaagtgatct acgtgcccag
       61 gcgcgagctg ccggccgcgg caatcactta atgaatgagg caaagcatat tgagtagccg
      121 gctggaattc aacggcaagc ccgaaagatt aaaaattagt ggccgaaaac agctgggcgc
      181 gcttttaagc taacctcaaa gggagactgt aatcgcgatt ccgcaaagct cgctgaatcc
      241 taatcaagtt gattatagct tcttctcatc tcgcccgtgc acaaatgccg gtggatgagt
      301 cgcttcaatg gcagtaaaag cttcgagaat gcgatagcta tggctggcgc cacgcggcac
      361 cacccatgag tcgccggctt ccagcagcac gagctgtggg cccagatgga gctcggcccg
      421 tcctttgatg acatagccga cggcttcata gtcccgctcg cttgccggtt tctcctcacc
      481 cggctgttcg tcctcccata agcgcatcgc catgcttacg ccggcagcca aatatttctg
      541 ccccatcgaa cctttaggag aggatttggc gtttactttt gtgatgcttg tttcggccat
      601 tgtcgtcctc cggattggca gtttgtttgc tgtttgaatt atcggcaatc gacaggcccg
      661 agtccagtac ggccaaacga ttaattccgt gcgccatcga tgcgcgaggc tgatctagcc
      721 gcaatcgaac gaccagctga taacgctcat ttgacagcga gaagagctga tgaaagaatt
      781 cggcttgctt ggcaggcgtg tgtcgaaagg agagtaaccc cgtggcagtt ttgcacagtg
      841 aagttgttcc gaaaaattcc gggcgggcct ttatcgtcaa gaaaggacag agagtgcgca
      901 tcgccggaaa gagcatcgtt gattttgtcg cgttcaatct tgacgattcg agcgagcgat
      961 ttgatcaggc gcggaccaag acaaatcagg tcaaaatctt tatcagcacg ggcgacatcc
     1021 ttttttccaa gcgcaacaac ccgatgctga ccgttgttga agatacattc accgaagggc
     1081 gtcacgatct tcaaaaaggc atgtgcagcc gcaaacggtt cgaaatggtg gctcaaggga
     1141 aagctaaacg agtgtttgcc gaaggtgtcg atatcaatcc taaggcgccc gacgatatcc
     1201 cggaccatgg ctgtttcgaa aacctgagcg atgcgctgag gcagtggaag atagcgcccg
     1261 aggatatccc cagcccgttt aatatatttc aatgcatgcg catcgatcct gaaaccggaa
     1321 tcatgtacga cactatgatc aggcctaagg acgaggctca tgtggatttt cgcgccgaga
     1381 tggattgtct ggttgccgct agcgcttgtc cggagtcggg cagaggacag gcaatccggg
     1441 tggaaattta cgatcaatag gagatgggga gggagtacct aacaaaacat aaaaacaccg
     1501 cagagacaca gagagcgcag agaaacattt gatttcgtaa gagccaaacc tctgcgctct
     1561 gcgcctcggc gctgaaaagc aactgctttc ttcgcaaact tccccgactc cacccattcg
     1621 ctagttacta atctcgaagg tcaacaattg ccgcggcgct ttttgatagg cctcgagacc
     1681 cgcttcggcg gattggccct ttgccggcgt caagagacgc gtgcgcacgg aaaattgcat
     1741 cgggctttcg tcaaacggat aaggggtcat ctttaccgag cccggcccgc tcggtgtaat
     1801 ctttagctcg gcttcttcgt tgctgtcgta agcgatacga attggcgcaa tcgtatactc
     1861 tttgaactcc ttttcggaag cgtagccgtc gcagcagaaa taaagtgata gcaggtcgaa
     1921 aatctgcaag gtgcggtagt tgtgccagag ttcgtcttcg aaagcaggat tttccgccgc
     1981 cagagttttt ttgtattgct cctgtttggc ctctaactct tttttcgcat tttgaacttc
     2041 ctggcttctt tcgcgcacat tgccggcagg tttcgtaaag acgttataac gattgtgcca
     2101 caagccggtg cggtgcatcg acacgatcaa acccgcatag aggtcgtgcg aacaaagcca
     2161 atcgacgttt ttggcatagg cttctagttc gacagcttca aacgagggga tattttctcg
     2221 atgggcgtac ggccggcctt tttcgacgtt gatgggcgga ttgccttcga attcacgata
     2281 gccgctgtcg tgataggtgg tggcaaagac cattgtctta taaggccgga gctgcgagaa
     2341 gcgctcgtta ccccagtgag ccgcgaattg cgccgctagt tcggcatggt cttcctgtag
     2401 gctgacgtaa gaacggccgt ctttgagttc gcgtataacc atcgtcgaag ctccttctaa
     2461 tcattgtttt gcggcattct ttaagcacaa ctctgccgcg agttgctgca atcgaggcgg
     2521 cgcgggtcgt cgttctcgag gacgtccgtt gtccatatgt tcgctgcgtc gtggaccgaa
     2581 gagacaaaga tcccgcgagt caaacaacct tcgttccact accggttcat agctcatttt
     2641 agtttgccgg agaagcggtt gcgattgttg ccgatgcccg gttacttgca ccgacagaaa
     2701 aatgcgatcc cgctgtcacg ggttgattgt caggtaatcc gaccatcctc cgaacgcaaa
     2761 ggaagaatca atgtacagtg tcccgtcagg atttgtcgat cctcctgatt cttgccttcg
     2821 agctcacagc taatccatgg atcagaagaa tcactcgact tctcggtaat ccgcgcgaaa
     2881 aaacgcatcc tatcgccttc gaatgtcgaa taggtgtggc gcacgttaat ttttcgcagc
     2941 cagccgcgcg ggccgatcca atccgttaac atccggatca gcagcgcctg gcgataggcg
     3001 ccgctcgcga taaacgttgg cagtccattg tgctggcgca cgtggtcacg atcaaagtgc
     3061 aggtgctgaa cgttttcttg caacccggcc caccgtacag tgtcaacgat cgtgagtggg
     3121 ccgtgttccc gcgggggaat cgaatcacca atttgggcct tatcaaagaa aatcgtttgt
     3181 tgcatgtcgc aagctttgct acttaagctc ccgtattaaa gttctcatgc ggcgggggag
     3241 cgttgccgag ttcacctaga atctcgttgt tgtgctggcc tagtcgtggc gccggagcaa
     3301 gctggacgcc ggttaccgtg gcgatggctc cgtgagggaa tataatttgt ccaagcagcg
     3361 gatggttcat ctctcttaaa aagccgcgcg cgacgagctg gggatcgtcg atgaggtcga
     3421 gcgtcgtcgc taccggcgaa gcaggaaagt gtcgccgctg cgcttcttcg accagttctt
     3481 ctttggtgaa acgcgtcgcc catgcatgga gccgatccat aatgaagtcg cgatgcttgt
     3541 gttgattttc ttcttgggcc aaagacggat tcgagctgag gtccggatcc tgtatccaat
     3601 cgaggaattt gtcccagcga cccggccggt gtatctggct gataacccaa tggccgttct
     3661 tacaaggcaa ggctccggaa atcgcagtga tggccccctt gctgccgcgg cgctcggtga
     3721 tcgttccaga aaaactgtac tctaccatgg cctgctcggc gaaagattcc aacgattggc
     3781 gcaccgacac ggtagcggcg atcccacgtc cgcaatttct agctgccagc aaagcggcgg
     3841 cggtggcgac gccgacatac gcccccaccg ctaaataagg gatgtgagcg cctaaaatca
     3901 tcggagcctg attcggctga ccggtcagtg atagcagtcc ggatcgcgcc atggcgcaga
     3961 tctccgggga gtcattttca atcttcgtaa ccaccaattt tggattggcc tcaaagcaaa
     4021 gttgctcttc aatcggcaac cgctcagcaa ccaaagcgtc acttcggccg agcaaatcaa
     4081 taaacagctt tcggccggtg ggcgaatcga agtcgagggt aaggctcttc ttacctgcgt
     4141 ttaaaaactg atgatacgcg ctgtgctcca aatccttgtg gtcggcgaga aaaggcggca
     4201 ggcggcgcaa atcgtcgccg ttggtctctt cgactcgaat gacctcgtgg ccttcttcga
     4261 caaaaagccg ggtggcataa gatcctataa ttccggtcag gtcgattatc cgcgtcatct
     4321 atttgtgctc gtcggcgcgg tcggaataag tcttgtcagg aaaaatcaca actgtatggc
     4381 gccggcgcaa gacgatttgc tcgtccggat tcaaaacttg ttcttcctgg gtaatgtaga
     4441 tgccgagtcc ggcgcggccc tcgcgggcga cgatgtcgac gatgcagctt cgggtgaaaa
     4501 gctgctcgtc gagatagacc ggcgcgatcc actcggtctc ggatccggca ttgcgcacgt
     4561 tggtataccc cggcggcaag ggtagctggc gccgcagatc catcggatcg gcgttagctc
     4621 tttcggtgct ctctctgaaa ctgaacggca cccaaccgac cagcgtcggt ggaagcagcc
     4681 gccctcgata gccggcggcc cgggcaaact catcatcgag ccacaaccgg tttgcatcgc
     4741 cggtggcgtc gacataacgc ctgacatcgg aggcgctgag aacttccagg ggccgcgccg
     4801 gactttgccg cccaattgcg gcgcgcagtt caggagtgat gattgaagga ctattctggc
     4861 tcacgtgaaa gccttatgtg tttctatctc gtcgaactgc tcgccggata gatttagcca
     4921 ccggcgcata acgtctgtat tgtgctcgcc caggctgggc gcggtggatg gcgacggcaa
     4981 tgaaacgggt gacagagtga agggcaagcg ggtcgtcagc atcgaaccga tgcgtcgctc
     5041 ttgcatgcgt gaaaaaactt tcgccggatc cggttcatcg atcacctgat cgatgcgccg
     5101 cacccgttca gcagggatgc cggcttcttt cagacgccgt tcgacttcag agctggaata
     5161 ctgctcagtc caggcttcaa tttggcgatc gatctcgtcg tgatgatcca tgcggcccgc
     5221 ggcgctgctt aagcgagtgt ccgaccttag tttttcattg cccagtactt tggccagtgc
     5281 ttcccactgt tcgtcgttct cgatgcttag ggcgcaccaa ttgtccgctc cggtgcagcg
     5341 ataaactcct tgaggagcaa ggatgtccga acgattgccg agccgatgtg gaaccttgcc
     5401 gtttaccgcg ctgctcagca acagcggccc gatcatcgac gcgctgcact cgaactggct
     5461 taaatcgata tgagtgccgc tgccggctcg cttgccaatt gcctgcaaaa tcgcgaaaca
     5521 ggcatgcagc ccgccaatgt aatcattcca cgagttggaa atcgcagtgg gcgggtcgcc
     5581 ttccgtgccg gtcaccgtca tcagaccgct gtacccttgc aggttcatgt tcatgctggt
     5641 ccaatcgcgg cgggggccgt tgtgaccata accggacatg ctgacataaa taattcttgg
     5701 attgagcttt tcaattgttg caaaatcgag cttgagcctg ctcatgacgc cggcgctgaa
     5761 gttttcgatc accacatccg caacggctgc taacttaaga gcgatttttt gcccggtttc
     5821 gttcttgagg tctatggcaa tggagcgttt gttgcggttg gtatttaaat aggaagcgtg
     5881 acgctggtca tcactgatat aggtggattg ccagggatgg tcgggatgct tgctgctttc
     5941 gacccgaacc acgtcggcgc ctaagtcagc aagcaggcgg gtgcagaacg ggcccgccag
     6001 gacgtgagtg aagtcgagca cgcggatttt gtcgagcggc gcgggaacag cgggatattt
     6061 agcaccaccg gcgttatcgg acattgagct ccttttgtgc ttgttgaagc agatcgcggc
     6121 tggctacctg gcttaccggg atgtctttct ttaggtttgc tcggtcgagt tcgtagcgta
     6181 ctagatccct gagcgcctga tcggacaatc cgccatcgac gctgtagcag ggcaggtaat
     6241 tgtcaaaagc cgcgcgtgtg gtggcgagat cgagcttcaa ccacttggaa gcgatggcga
     6301 cgctttcttc ctggtgatct ctgataaacc taagagcctc gatggttccc ttgattgtgc
     6361 gctttacctg ttcaggcgat tgcttcaggc tcttgtctgt tacgaccatg gcgttttgcg
     6421 tcagcggaac atggtcacgc gtccagagca gaactttgta gcctctcttg gcgccgaata
     6481 tatctgccgg cgatgagacg aaagcggcat caatttttcc cccatccatc gcggccagtc
     6541 gggcggtatt atctcctccc ggtaaaagat tgacgtcctt accgggttcc atcccggctt
     6601 gacgaaatag ggttacgctc gtcagataca actggctgcc aaaggtgctg ataccgatgg
     6661 ttttcccgcg cagatcggtt aacttctgca cctccggttt gaccaacagc aaatagccga
     6721 gtttgtcctg tgtggtcatc accagcttga tcggcagtcc ttttatcgca ccggcaatcc
     6781 ctggtccggt gcacactgcg gaatccaggt cgccggagag aagagcgttg gtggcaatcg
     6841 cgcaagggac caatacatgc tcggagttga ttccatgctt actgtagaat cctttccttt
     6901 ccgcgaggac cgtgggcagg aagccaagcg aggcggccga aattccaatg cgaactcgct
     6961 cctgagagcg cgccggaatt tgcggccaaa caataagcgc gaacagagtt aaaccgagtc
     7021 gggtcgcatt tttgatagtc atttgtctgt tctcgtgaaa aaaccttcct gatctatttc
     7081 tctgatcaac gacggatcga aaaactctgc cgccttcatc ccgtcgagtt tgggagtcga
     7141 ttccctgaga tcctcgatgc ttgcctgaat ggcatcctcc cgcggatacg gcttgccggg
     7201 caacgcctcc agaaactgaa ctctgtaggt aaactccagc agcttcgggt tatcaacccg
     7261 catgtatttg cggaatacct gataggcgag ttctttgttt tttctgccgg ccgcaatcgc
     7321 ctcactgtat gccatgaaaa aggccttgag ccgcggccga ttgtctttca gaatctgtcg
     7381 ggtggtattg atctcgcttc ccggtgaagg aacgccaaag tccttcggtt cgccgagcac
     7441 tgagatcttt tgcccgctca attcggtaaa ttgaaagact ttgtcggatt cgccgatggt
     7501 ggcgtcgatc ttaccctgga acatgagttg tattcttttg tccgattcgc tcacgccggt
     7561 gggaaccagc gacacgtctt tacctgggat gaagcttagc ttggcaagga ttcggcgcag
     7621 gagaaaatcg gtgccggcgc cgatgcggct gctgccgacg attttacctt tcagctctgc
     7681 cgtggatttg atcgaaggca ctgcgatgag ctggtacggc aatgtgccgt tggtggaaac
     7741 cgttacaatc ggcgcgccgc gggcgactgc gcccacgacg cccgagctgg aatcagtggt
     7801 taaatgaaca ttgccggcga gtagggcagt cagggatacg ctgcctgcgg caatgctgac
     7861 aagttttaca tcgagcccat atttttcgta gagtccgaga tctttagcaa tccacaaagg
     7921 cgcgcggttg ccggtgaacg aactatagga gacaaccagc ggttgaagtg tcctttcttg
     7981 cgcctggcct aagcctgcaa acgatagggc aagcaacaga atcgcagtac gcgaaaacat
     8041 cgaacctccg gtatgaaatc caaaccgtag agtgatgcct ctgtttattc atcacccggc
     8101 catgcgtcct tctatacatt ctttttccca gggcaacaac gtggcgcctt caggttgcaa
     8161 ggctaacgcg ggtggattaa aaggagcttt agtgaatgca aacatgcatc ggtgcagacc
     8221 gtgagaagtc attaatcaat caggatgggc gaacgaggag gttcgaaatg gagctgcaac
     8281 aacatcattc tcgtggcaac gtattctggc ccggcaacaa tcgcatcgcg gtttttctaa
     8341 gctttgattt tcaaggtggc gaagatgttc ggcccgacaa aaacggcagg gtcaatcatg
     8401 aagagtacac ccaagctgaa tacggtccca ataccggaat ctggcgaatc ctacgaatcc
     8461 tttcggccca tgatgtgaag gcaacgtttt tgacctgcgg tgggatcgcg gaaagatatc
     8521 cggaagcgct ggcggcgatc gtcgcccaag gtcacgaagt ggcggggcac ggctatcatc
     8581 atgaagttgc ccgcgatctg agcaaggatg aggagcgaga ggtgatgaag cgaaccagcg
     8641 agatgctact taaccgcgcg caaaaaccgc cggtcggatg gcgctcctgc acgcaaagcc
     8701 cgaacagttt ggaattgctg atggaacacg gttacttgtg gaacagcaac tcgttttcgt
     8761 ttgatttacc gttcgtttgg cgaaacggag acaagcagct cgtcgaatta ccgcgacagc
     8821 cgtttggcga cggcaggacc tacggccatc gcgattcagg caatccaaac gataccttaa
     8881 cggtttggaa agctatgttc gaccagttct atgaagaatc gaaaatagcg ccgacgttct
     8941 gcccgttcca atttcaccct tatatttcta gccggcccgg tcgagccaag acgctgtctg
     9001 atttgattca atacatgaag tcacacgaag gtgtctggtt cgccaccggt agtgaagttg
     9061 caaaatggtg gttggataac ggcttttcag ccgaggcagc agcgcaggta tccaaggtag
     9121 cttaaagcta atgagcttgt ggcgcttgcc caagcgccac aagccaacac gacttgaagg
     9181 tcgaatacag atcgtccttg cacaaatccg gtccttatca gtaggatcgg aataaaaatc
     9241 tcactctctt ccgcagccgc ctccccatcc gctttttcac tgcgaaccaa acccgcaaca
     9301 gccaattcct ggagaaccag gcaaacgagg tggatgtcgt aatccactga tgattgaatt
     9361 caaagcgcgc tttgaatttt ctgatttgca gggttttgcc gaattttctt ttgaagatcg
     9421 ttctgacgct gaccgcgttg cgcgtttcaa cgaaaacagt cagcgtttcg atcggcaaca
     9481 gttggcgcac gcgcgaaatc tttagcgcca gcgtctcttc attgcgagcg ctgatctcga
     9541 ttagattgtt caacagttcc ggatctacac cagctttgtc gatgacttcc ctgcgcgccg
     9601 aaagttcttc gcccccatca tcggtgtaac ccgcgacgat aaaataggtc ttgtgcttgg
     9661 aagtctgcat cgacttctgc caccagtcga tggctttctc aagaccgatg cgtgatgctt
     9721 gtgaaagatt ccttgaaccg gcgcagccgt agggcattgc tacgatcaca atcgaaatgg
     9781 tcgtggtctc cttttctgtt agcttagcgg cgggtaaagg tcaggatcgg caaatcgtca
     9841 cggttaccgg cttgccgcct gaccgcgaga tcgaacgcgc gttcagtgtc accagcagat
     9901 ccatctgctt cgagcaaacc tgcctggcta ttttgccggt gattcattta ttcttatctg
     9961 ctgatttgaa tgttgccgtg atgcggtaat gctttcgcca tggtttcctt cgccgccaac
    10021 cggtgtcgtg aggcatatgc tcttcaagat ttgttttcac gttttaagcc attttacgga
    10081 ggtgggcgtg cggcctgacg ctccttataa agattatcga cgaaaccgct ggaggccagt
    10141 tcacgaacga atttcgattc cacgaacctg tcgggctcaa tcccgggcgg tatcggccgc
    10201 gttgcagcga gctcgtctaa gatcgtttga attcccgcct gcgtagcttc cgggactctc
    10261 ttgaaatgtt tcgtagcgta gacttggtaa attttctcga cggcaggggt ggttttctgc
    10321 ttggtatact tctcgagtgt cgcgagcgca gttcttttgt cggttttgaa gcgatgaatg
    10381 ccctcgacat aagccttcat aaagcgcgtg atcagttcgg gttctttctt gagaaacgac
    10441 tggcgcgcgg cgaagggccc aaacggataa accaattcag atggaacatc ggccagacga
    10501 gtgtatcctt cgctttcggc ccgcagtgtc tcgggaccga tcgaaagcac gccgctctga
    10561 accgagccgc tcttcagagc ggcaaagact tgcggcccgg aaccggcttg aaaaatgccg
    10621 acgtctttgt cggcttgcat accccatttc ttcaacagat agcgcgccac aaaatccgta
    10681 gtcgcgccga tgcggctgac cccaagcttt gtgcctttga gctgcgccgg ttgtttgatg
    10741 cggggaatcg accagagttc gaagggcagc gaatcaatgc tagcgccgag cagcacgaca
    10801 tcggaacccg agagattggc cgccacggtg gcacccgcag cggtgaagga gatggcgaca
    10861 tcgccggcaa tcaaggcttg caacgacggc ggtccgccgg cgatgtagat gaattccgga
    10921 tcgatgccgt gcttcttgaa gattcccgcc tcttgggtga tccagagcgg gccgtaaacc
    10981 acggcaatgg aggtgtaggc gattcgtaga ggctcggcgc cggaacttgt tgagaggctt
    11041 agaaaaataa tgatcagcca gattcggccg aataaatatc gtaaataaac aagaaaattt
    11101 atccgatttt ctctatccat tcgtgggtgc tcgtgagcaa tctgtaagtt agattaccct
    11161 cttctgtcaa gtaattgaag ctgtggtcga gcgggcggtc aacttaggat cgtgctttcc
    11221 agaactcgga taggcagcgc tttctgtcag cgccgctaac tgtaaacatt tacggctctg
    11281 ccgcgtccaa cactctgaaa gacaatccat tcgagaaagg atagacatga agaaagcaat
    11341 tgtgatcggt ttcagcgttt tagctcttgg cagcgcagta acggcttccg ctgacgatcg
    11401 ctcctatggc tggcgcggcg accgaatcaa ccaacgtctg gacgctcgcg gagcgcgcat
    11461 caatcagcat ctcgatgcga ggggcgaccg cataaacgac aaattggatg cgagagcggc
    11521 aagggctaga gccgatggca agttcaaatt agccgaacga ctcgatcgcc aaggcgaccg
    11581 gatcgagcgc cacctggaca agaaaggcga ccggattgaa gcttattggg atcgccgggg
    11641 tgatcgtatc gatcgtagat tcgaccggcg tgatcaccga gatggccgct tcgcccataa
    11701 cggctatcgc cgctgagcat gagcactggc aagagagttc cgggaaaact tcccggggct
    11761 ctcttgcctc gtgtgttggc cgaggtgatc gcgccgcgct tatcccgaaa aatattcttt
    11821 aaagccggtc accataatgt ttgccaatct ctcattgtaa ttctcgacgt gtccgtagtg
    11881 cgtgaggttg gggacgtgaa gcaatcgcag cgccttggta tggggagcaa aacgattgaa
    11941 ggcataaacc tctcggcgaa actccaggcc cttcttgccg ccctcgaccc aatgaccttt
    12001 atcgtagtcg ccgactatgt ggagcatttt cttgccgcgc aggcaagtca agtcggattt
    12061 gtaagtgatg aagagttcct catccggcag gcccgagatc ctgcgaatct ccgggagcaa
    12121 aatatcgtgg gcgctgtgtt cgatatcttg caaaaacggc ttgaactggg gccggcgatg
    12181 gttctccaac tcgaaccact tttcagcgct gccccagggt tgattgggtc cgacataccc
    12241 gctcttgatg tagccgcccg gagagcggcg gcttagatcg gtcaaatttc gaaacggcgc
    12301 ggcgcgctct tccgcagatt tctccgcaca cgcgagatcc cattccttgc gcacccatgc
    12361 cggcatgccg gtgccgaaac ccaagccgcc gatcaagttg tttttaagtc catactgttc
    12421 catcaagtaa aaatattcac cgccggtgga atgcccccac atgaaaatat cttgaccagg
    12481 caaacttcgc tccaccagcg ccttgatcgc ttcggtgcac atgcgaaagg tataaaccgc
    12541 cagccgattc ctgagttctg cgtccccggg agtttcgccg atgacaaact cggggcgtcg
    12601 ctcgccaatc ggcaaccaag gttttcggct atagtggccc ggcagcgaga gggccagcac
    12661 cgggataccc agagaggcga tatgctgcgc aacgccgacc cgtgccatgg ccggatcgat
    12721 ccgggtcaga tcgggatatt cttcgggacc atccggggtg aagataaact cgtactcgtt
    12781 tgccgcgccg ccgtgaatca tgaccacgcc gatagggccc aaagatgcct tggccggcgt
    12841 gaacaggcgg ccgtggatgt cccaccgaaa gagctctccc ggtttctcgg gattggcgta
    12901 ccgataaatc gtctgttgca cttcttcgac ggtaaacgca gtccgcgcga aaggatcggg
    12961 gagtgtctta taatcatcaa aggtcatgta gtagataaag tcgtcgcgta acccggcgcc
    13021 atgcgttagc gcagcaacca gcctgttcat tgcgtcttgg ctcatttatc ctccttaacc
    13081 ggcacactag cgtgttattt cgacccctac atatgttgtc acctgctgcc tatgtcaacc
    13141 gcagccccag ttcagatcct ttcatcctgg attgccctcc ttgatggatc ctggagaact
    13201 aggataaaga acaggaaata ggccaaggag gctatcgaat gagcggagat tcccaaaaag
    13261 catcatacgc aaaagtagac cgtaaagccg aggtgatgga caccccttat gatcgttgga
    13321 tcgcgtcgca gggcattgat gtaattcgag ggttctttgt ggaaaacctc tatacgattc
    13381 cgctcaaatg gtgggaccgc ctgggcggca acggcgttta tatcatgttg gacggcactg
    13441 ggtatctcga tgatgcttat gtttgcggca tccccccggg taagagcctc aatccccaac
    13501 gacatattta cgaagcgctc atttacgtcc tcgatggccg gggagcgacc accgtttggc
    13561 agaccaatgg gagcaagcaa agttttgaat ggcagacggg tagtcttttc gcgattccgt
    13621 tgaatgcctg gtaccagcat ttcaacggcc agggtgaccg cgatgcccgc ttgcttgcgg
    13681 tgaccacggc gccgctggtt tttaatttat ttcactgcga agattttgtt atcaacaatc
    13741 catattcctt taccgatcgg ttcaagggcg aagagggatt tttcggcggc aagggcacgc
    13801 tctacagcga ccgcgtggtc gagaccaatt tcgttgccga taccatcaat atcgagccgg
    13861 tggcgtggac cgaacgcggc aagggcaacg cgacgatttt cttcgagatg tcggacagta
    13921 tgatgggctc gcacgtttcg cgctttccgg tgggtatcta caagaaggcg caccgtcatg
    13981 ggccgggtgc gcatgtattc attctcaccg gtaagggcta ctcattactt tggcccgaag
    14041 gcaaagagat gacccgggtt gactggaagc caggttccat cgtcgtgccg ccggaaggct
    14101 ggtttcatca gcatttcaac tccagcgccg agccggcgcg atatctagct ctgaaaatcc
    14161 tcagccgaaa atacaagctg cagccgggaa aaatccagtc cgatgtgccg ctggcgttcg
    14221 gcggttggca gatcgagtat gaggacgagg atccgatgat ccgcaaaata tttgaggagg
    14281 aatgcgccca gtcgggtgcg gaagttcgta tgcccgcaat tgcccgtgac tgatgttgcc
    14341 cgaggctatg gttttgctaa taattggata aaataaccac aagttggact acaggagaaa
    14401 acgatgttga agcccatcgg attgaccgaa ggacattatg agtgccgctc gttggatgaa
    14461 acgctgccga ttttcaccga tctgctggct ctcgaagtcg tcgagcgcca agaccacgga
    14521 gcggtactga agcatccgaa tacggcgtgg cgtctggtgg tccatgaagg cggaccgaac
    14581 gcggttaaca agccgcacac caaccattac ggatttcggg tttcaagctc cagggaaatt
    14641 cccaaggcct acgaatacct caccgaccac aaagaccagt acaaaatcaa aagcgtgacc
    14701 aaacctcatg aagctcattt cgcctattcg gtttacttca aggagccggg tggtaacgat
    14761 ctcgaaatcg aatactacaa tcccgcggcc gtcaagcagg gccgcaaaat cgccgcgccg
    14821 cattggactg agccgctgcc ggaagagaaa tttcccggaa gggggtacgt gccccaggcg
    14881 atgacccatg gaactttgaa ttgcgatgac aaggaggtca atgatcgctt tgtcaaagaa
    14941 gttctcggct tggagattat cggcggcggc cgtacttcta cttacatcag tcatccaagc
    15001 acgccttggt atgtcgttgt tctgccgacc actcgacggg agtacttgac ggcagcgaat
    15061 cggtttacct taaaactggc atcggccgaa gaggttgccg atgcgcacaa ggaatttacg
    15121 accaaaggtc aagcgatcgg catcaatgag ctatgggacc tgcaagaaaa cggacgcact
    15181 cattttatct tcagcgacat cagcaaaaat tggtgggaag ttacttcgta actgcaaatc
    15241 gccgtttaac ccgtcggcaa tccggcggga gttctatcca gtgaaggcga ccagcccgca
    15301 gcccgtcgcc cacgggtgaa ccggctcata ggcgagcaag gttgcccgcc gctccccgac
    15361 ggctcctgcc acggttatcc aggtcctgat ttcgtggccg ccggaaccgg cctctttatc
    15421 gatttctgaa tgagagtatt cggccagacg ggcgcgcctg ccgttgatta aagttttgag
    15481 gaattgctcg tcaaaagcga tattgatttt gcccatcttc gcttccccgg gccagtgaga
    15541 gagaccgccg gtggcgacca aggcaattcg tttgggatgt tcctcgagga ttttaccgag
    15601 cacccggcct aattcaaaac agcgcttggg agcgggcatc ggagtcgtca aggtgttgat
    15661 aatcaccggc accaccggca cggtcatgcc aggatttaaa aaatgcagcg gcaagatcgt
    15721 cgcatgatcg aacaatagtt catcggaaaa agccggatca aagccgctat cgagacatgc
    15781 atccaatagc tggacgccca aagcagagtc gccgggcacc ctcgtcttgg gtattttcag
    15841 ccattcttcc accggcccat cgtagtagct ggcgcggccg atgcagaagg tcggcatctg
    15901 gttcaagaaa aaatttgccc agtgttcgac gctaacgatg accagagcgt cgggtttcga
    15961 agccgcgagc atctcgccga gtttgtgata gctgccgaga attttatccc tttgttcttg
    16021 gggcgcggcc tcggtccaag cagtcattcc cggggcatga ctcgcagcgc aggcaaatac
    16081 gatggacata gtttactcca atccggccag cgccctggcg taatcttcat tggagacacg
    16141 gtgcagtaac gtgaacggcc gcaacagcag cggatgaacg ccgcaggtgt agagtttgcg
    16201 cacatccaga tttgctatcg ccgcttgctc ctccgccgtc agttcgtagc gagcgagaaa
    16261 tccggcattg tcggctcggt aagcatcgcg ccgctcggga ttgcgattta cgtcgtagat
    16321 gagtttctgt agttgataga gactcatatc aaccgtccaa tctttggtgc agccaagtga
    16381 ttaccgtttg aaaaatattc gggatagcgc ccatatgacc gccgggaaat agccgaatcg
    16441 ttttcgggtc gccatgctcc aggagtagat agaaatcctc aagcggcact tgcttatctt
    16501 ctttaccgtt gacgattagc aatggtgcgc acggtttgtc caaccagcct tggtcgagca
    16561 gcgagagcgc cggcataact cggcagagtt cgtcgaagct gttcatgccg aagagatggg
    16621 cgcgcgcttc gatcagatca aagagataag agtcggcgtt gcgcgatcgt tcttgccatt
    16681 ggggctgaaa gaagtagtgg atgcctccgc cccagttcac cgccgctcgt aatttgtcgg
    16741 gcgcgagatg ggcaagtttg gtcgaccagt gaccgccgaa gctggcgccg accacggcaa
    16801 tgcgcgtcgt atccacttcc ggtctttttc tcagatactc caacgccgtt gtgtgaacct
    16861 ggtgcgcatc gggtccggcg agcaccgggc tctcgccggt tcccggacta tccatcacga
    16921 agcaacccca gccttgtttc acaaacgatt cggcaaagga gtggcgttcc tccttccaat
    16981 tgtcgatgcc gccccaatgc ataatcatgg gagcgggcac tcttttaggc agccgcagat
    17041 gtcccacgat ctgtttgtca tgaaacggaa tggacacacg ctcgatggga atttcaaaat
    17101 acttcgaggc agctagaaac atctcgcggg tttttacata accgtgatgt ttacccggag
    17161 tgctggggaa gggatggcgc gcaatgccgt agtagccgta ggatttcaga taactttcct
    17221 tggcttcttg tttattgccg gcgctctcgg ccgaccttgc tttctcttcc cagcgcgcgc
    17281 ccaattcatt ccacgcctgc gcccatgtct cgccttgcac cgaactcatc ttaccgaggg
    17341 cttcttccgc ttcggcttta tcggcatgta gaaacggcgc tcgctggccc aggcgagcgc
    17401 gaatgtcatt cttgatctgt tccaggctac gtggctgatt catacactcc tcatcttcgc
    17461 atcataaagt atgatcgaaa acatagatcg gctgggggca taaatgcaac gctgcagccc
    17521 atttgcgcca caagggccgg gcaggcgtta tttcgtggtt gcatcttcga atgttctatt
    17581 cgctcaaatc catctctaaa gtcttttttg catggcaatc aagaggctat ccgtcgacta
    17641 acggaaaccc ggaaaaatca aattttataa tcagtttgca ccggcaacta gcggcgatta
    17701 gggttgacgt ttagcttagt gcgtggcatg ccagtaggaa cgtcgcttct ctagaaatgt
    17761 tttttcttcc atcacacttg caatcctcaa gaggtaggca tggcatcaat cctcgacggc
    17821 aaagccgtgg ccaaagaagt tcaaaagaaa atcaaagagg aagttgacgg gctggagcgc
    17881 cgttggggca tggcgccggg tttagctgtt gtattcgttg gcgatgatcc ggcgtcccac
    17941 atttatgtaa agaacaaaga aaaagcctgt aacgaagtgg gaatcatgtc ttttgagcat
    18001 ctccttcccg cgactgtttc ggaaaaggaa ctgcttaccc tcgttcacag tttgaacaag
    18061 aacaaagatg ttcacggcat tctggttcaa ctgccgcttc ctgctcacat ccgctcggaa
    18121 agaattctcc aggctgtatc tccgttcaaa gatgtcgatg ggttccatcc tatgagccag
    18181 ggattgctgg taatcggcgc ggatggcttt cgaccgtgca cgccattggg agttatgaaa
    18241 ctacttgagg ccgcggattg tgctctcaaa ggcaagaatg ccgtggtggt cggccggagc
    18301 aatatcgttg gcaaaccagt agcgctgatg ctgcttgaaa agcatgccac ggtaacaatt
    18361 tgccactctc gcaccgccag cctgcgcgct gaggtagcca gggcggatgt tttagtcgtg
    18421 gccatcggca aagcaggatt ggtccgaggg gactggatca agccgggcgc cgtggtcatc
    18481 gacgtcggcg tcaatcgatt accgagtggc aaactcaccg gggacgttga atttgaagcc
    18541 gctaaggaac gagcatctgc aatcacgccc gtgcccggcg gcgtcggtcc catgaccatt
    18601 tgcatgctgc tgcataatac tcttaaggcg gccaaggact cgatccagcg ggaacgttag
    18661 ccgatccagc ttcggcttca gcttcgtgtt gcgacccatg aacagaaccc cgttattcgc
    18721 cgctcatcgc cgcgccggcg ggcgcatggt tgagtttgcc ggctgggaga tgcccatcca
    18781 atacagcggg atcatcgccg agcatatggc ggtgcgcagc cgggccggaa ttttcgacgt
    18841 cagccatatg ggcgagatcg cggtgcgcgg cgccggcgcc gaagcgatgt gccagaggct
    18901 tagcgccaat gacgtcgcgc gcatgcaagt atcccaagct cagtacaacc tgtttttgaa
    18961 tgaccgcggc ggggtcattg atgatgtcat actgtataag cggcagccgg acgattttct
    19021 tatttgcgtc aatgcctcga acagtgaaaa agatttccaa tggctccggc agcacgtaac
    19081 cggagaaatt caagtggaaa atgtcagcgc ggactatgcg caattggcat tacagggtcc
    19141 gttggcagaa gaaatattgc agccgttaac agcgatggct ctcgccgaga ttcaatcttt
    19201 ctattttgcc gagagcgaag ttgccggcac gcgctgcttg atcgcgcgca cgggttacac
    19261 cggcgaagcg gggttcgagc tctactgtac tccccgagac gcggaaaagc tgtggcaagg
    19321 tctactcgac gcaggctcgc cggcgggttt agtgccggcc gggctcggcg cgcgcgatac
    19381 tctgcggttg gagaaagctt atccgctcta tggtcacgaa ctcgacgaca gcaccacgcc
    19441 gttggaagca ggactacaat ggctggtgaa attttccaaa ggctctttca tcggttgtga
    19501 agtcttgcta aggcagaaac aagcaggcgt caaacgcaag ctcgtcggct tggagctgat
    19561 cgaacccggc atcgcgcgca ctgattaccg gttgttgaaa gatggacggt gtatcggcag
    19621 agtaaccagc ggcacccgtt ccccgacact cgggcagtcc atcgcgcttg gatatgtcga
    19681 ggcgaaagaa gcccgcgccg aaaatatact tgaagtcgaa atccgcaaca aaccagttcg
    19741 cgccaggata gtgccattgc cgttttacgg gcgctgacac ggcgttagca ggccaaggag
    19801 gaaagatcat ggagattcct gaggggctca gatattcaaa agaacacgaa tgggttctgg
    19861 tcgagggtaa gacagcaacc atcggtatca ccgaatacgc gcaggaggaa ctgggcgaca
    19921 tcgttttcgt cgagttgcca gaagtcggtg aaaagatcgt aaaagatgat ccgttcggcg
    19981 ccgtggaatc ggtcaaagcg gtgtcggata tctatgcgcc tgtaagcgga acggtattgg
    20041 aaatcaacga ctcgcttccg gacagcccgg aaaccattaa cgatgatgct tatggagatg
    20101 gctggatgat tcgggtcgag atgcaggaca tggacgatct taaagatttg atgaccgctg
    20161 aagagtacgc cgaatatatc gagcagcaga aggaagaaga cgatgaggac gacgagaccg
    20221 aagacgatga cgaggaagag gaatagtctc atcgaatgag ctacacgccg cataccgagg
    20281 gcgacaaaaa tcaaatgctt gacgcgatcg gcgcgcgcgg catagaagat ctgttccgcc
    20341 atattccgga gaaatttcgt tcaacggcaa aaatcgatct gccggacggc atcagtgaat
    20401 tagcagtccg gcaacggctt gccgctctcg ccgccaagaa cgctgcagcc aacgattggg
    20461 agctctatct cggcggcggc atctatcatc acttcatccc aagcgccgtc gacgcgatta
    20521 tttcacgctc cgagttctcg acctcctata ctccttacca gccggaggtg agccagggta
    20581 ctttgcaagt tcttttcgaa tatcagacct tgatctgcca gctcaccggt atggaagtct
    20641 ccaatgctgg cgtttatgac ggcgcgtcgg caaccgccga agcgattctt atggcgcggc
    20701 gcattcaacc gccaacccgg cgcaaggttt tggtgtcgcg cgcgcttcac ccgcagtatc
    20761 gcgctgtgat tgctacttat ctccgcaacc tttccggcct gaccctcgag gaaattcctt
    20821 ttgacggcac cggcgcaacg gatatcgacc aggtcgcgcg gcagttaaac gaacggacga
    20881 tgtgcgtgct cgtaggttat ccaaattttt tcggcgtgat tgagaatctt gcgccgctgc
    20941 atgagctctg tcgcgctgcc ggcgctcaac tgattaccgc caccacggag cccttggctt
    21001 ttggggtgat caagccgccc ggagcatttg gcgtcgacat tgccgtgggc gaaggacaaa
    21061 gcctgggagt gccgatggcg ctgggcgggc cagggttcgg gttctttgcc agccagcgaa
    21121 aattcatgcg caatctgccg ggcaggcttg ttggcgagac tgtcgatagc gaaggccggc
    21181 gcggctatgt tttgacgctg gccacacgcg agcaacacat tcgccgcgaa aaggcgacat
    21241 cgaatatctg caccagccag acgctatgca cgacggctgc aactgtttat atggccttct
    21301 tgggacgcag cggcatgcgt cgtttagctg aggtcaatgt agcacgtgcc catgaaacgc
    21361 atcggcgcat aatcgaaggc ggcaaattcg aaggctgctt tcccgcgccg ttcttcaatg
    21421 agttcgtggt caggggaaaa cagctcgatg agatcatgca tcgctgttta gcgcgaaaaa
    21481 tcgtccccgg tattccattg gcgagatggt atcccgagct accggattgc ttgcttgtgt
    21541 gcgtcaccga gatgaacgac agagaaggga tcgatcgcct tgtccgagcc atctcgaact
    21601 gatccgcgac agcccaatca gttttcgcgt ctgctgttcg agagcggctc acgcggaaga
    21661 accggctttt cgtggaccga gaaaacaccg gcggtttcgg acagtattcc cgaagcatat
    21721 ttgcgtcggg atatctccga ttttccagag ctgggagagc tcgagctgct gcgccacttc
    21781 actcgtttat cgcaatgcaa ttacgccatc gaaagccatt tttatccgct cggctcgtgt
    21841 accatgaagt acaatcccaa gatcaatgaa gtggtggcac gatatcccgg cttctcgctt
    21901 attcatccgc tcgcgccgcc ggcgctgaac cagggcgcgc tggaattgct tttcgctctc
    21961 gaacggatgc taggcgaaat cagcggtatg gatgatgtca gcctgcagcc ctcggcgggg
    22021 gcgcagggtg aattaaccgg cttgatgttg atccgcgcct atctttcagc gcagggaaag
    22081 ccgcgcaaga agatcattgt ccccgatacc gctcacggga caaacccggc gagctcgacg
    22141 ttgtgcggct atgatgtagt gcaaatatca tccaatgagc acggcgtgat cgctgccgcc
    22201 gcagtcgccg atgcgatgga cgacgaggtc gcggcattga tgatcactaa cccgaacacc
    22261 ctggggatat tcgagaagaa tattgaagcc gtcgccgacg ttgtgcacgg caaaggcggt
    22321 ttggtttact tggacggggc taatttaaac gctttgatgg gaatcgccaa accgggccat
    22381 atgggtgtcg atgtgcttca tatgaacttg cacaaaacct tttccacccc gcacggcggc
    22441 ggcggacccg gagccgggcc ggtcgcggta aaaacgttat tacgagattt cttgccgacg
    22501 ccccgaatcg taaaaacgac tgatggttac gacttgatcg aggacatgcc ggcatcgatc
    22561 gggcgcgtgc ggtccttttt cggcaatttc ggcgtgctgg tccgggctta tgcctatatt
    22621 ctatcgctgg gcgccaatgg gttggaagaa accagccgaa tggcgctgct caacgccaat
    22681 tacatcagga gcaagttgga agggcattat cagctcgcgt acaacgagcc ctgcatgcat
    22741 gaatgtgtct tcaacgaccg caaccaacat aa
//
LOCUS       DASUVS010000475         3470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_430582, whole genome shotgun sequence.
ACCESSION   DASUVS010000475 DASUVS010000000
VERSION     DASUVS010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430582"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VF182_25000"
     CDS             <1..309
                     /locus_tag="VF182_25000"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX6440401.1"
                     /translation="SALFSSHQKRIASLATKWRIPTIYTRGESVDHGGLMSYGADQDE
                     SVKRIAMMIDKILKGAKPADLPVEQSTKFDLVINLKAAKQIGLTIPPSVLARADKVVQ
                     "
     gene            367..1410
                     /locus_tag="VF182_25005"
     CDS             367..1410
                     /locus_tag="VF182_25005"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440402.1"
                     /translation="MKALSLKSLSESPKSAIQIRKWAGLAAIAVALTLYGAMAEAQQP
                     KKVYRIGYLLPGDRTSESADSDAVRLALRELGYIEGQNIALEYRYADGKRDRFPELAA
                     DLVRLKVDIIVVSGGNTPVWAVRNATRTIPIVMASGSDPIQAGLVESLAHPGGNVTGL
                     TNLRRELGGKRLELLKEAVPNLGRVAVLYYPANPNSVREAKEVLPSAARALGLTIQPW
                     EVRDANGFDKVFAAFNKQRPDGLYVPGGTLVTTNQKRIVSLASKSLLPAMYNSRDAVE
                     AGGLMYYGADLSDSHRRVAYYVDRILKGARPADLPVEQPSKFELIINLKAANQIGLTI
                     PSRVLERANKVIK"
     gene            1547..2479
                     /locus_tag="VF182_25010"
     CDS             1547..2479
                     /locus_tag="VF182_25010"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440403.1"
                     /translation="MGAIAEAQQQAKIPKIGWLGIRAAASDPARESFRREFRALGYIE
                     GKNIVFEYRSADDKIERLPALADELVRLKVDVLLAWLTPAAVAAKNATRTIPIVFNGG
                     FDPVAAGLVDSLARPGGNITGFSGIAPLLAGKRLELLKEISPKLSRIAVIWDPQNPGS
                     AQQWKESQLPARELEVQLHSIEVSSVDEFEAAFKAAIKSRSDALALMASPFFYSNQKQ
                     FVELATKNQLPTIYPREEFITAGGLISYGSDGKESFKRAASLVDKILKGAKPADLPVE
                     QPTKFELVINLQAAKQIGLTIPQKVLARADRVIK"
     gene            complement(2494..2880)
                     /locus_tag="VF182_25015"
     CDS             complement(2494..2880)
                     /locus_tag="VF182_25015"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX6440404.1"
                     /translation="MIYLDTSVALAHLLAEDRAPPDRLWQEPLISSRLIEYELWTRIH
                     ARKLSRSHADEVRALLSRLALIELAAPVLARALEPFPKLVRTLDALHLASMEFLRSQG
                     QYVTLASYDERLTNCARALRFPLYKL"
     gene            complement(2877..3167)
                     /locus_tag="VF182_25020"
     CDS             complement(2877..3167)
                     /locus_tag="VF182_25020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX6440405.1"
                     /translation="MKAVGLKILKNKLSEYVRLAEGGETVLVTDRDRVVAEIVPPETT
                     RSPVLADALLAEAVRKGWITPPALAGSTPPPRNPVTSFRKLQKELEQDRSDR"
BASE COUNT          820 a          910 c         1013 g          727 t
ORIGIN      
        1 agcgctttgt tcagctcaca tcaaaagcgc atcgcgtcac tagcgacgaa atggcggatt
       61 ccgacgattt acactcgggg cgaatctgtc gaccacggcg gcttaatgtc atacggggcg
      121 gatcaagatg aatccgtaaa gcgcatcgcc atgatgatcg acaagattct caaaggcgca
      181 aagcccgcgg acctacccgt cgagcagtcg accaagtttg acttggttat caacctcaaa
      241 gctgccaagc agatcggcct cacgattccg ccgagcgtgt tggcgcgggc ggacaaggtg
      301 gttcaatgaa acaatttcgg atttttggat ttttgatttt ggattgaaag cgagaagaac
      361 aaaccgatga aggcgctttc tttgaagtcc ctttccgaga gtccaaaatc ggcaatccaa
      421 attcgaaaat gggcggggct cgctgcaatc gctgtcgcgc tcacgttgta tggggcgatg
      481 gcagaggcgc agcagccgaa gaaagtctat cggataggat atctattgcc gggcgatcgc
      541 accagtgagt cggccgattc cgacgcagtt cggctcgctc tgcgcgagct tggctatata
      601 gaaggacaga acatcgcatt ggaataccga tatgcggacg ggaagcgcga tcggtttccc
      661 gagcttgcgg ccgacttggt gcgtctcaag gttgatatca tcgtggtgtc gggagggaac
      721 acaccagtct gggcggtaag gaatgcgacc aggacgattc ccattgtgat ggcgagtgga
      781 tccgatccta tccaggcagg tctcgttgaa agccttgctc atcctggcgg caacgtcacc
      841 ggcctgacga accttcgcag agaactaggc gggaagcgtc tggagctgct caaagaagcc
      901 gttcccaacc ttggccgtgt cgcggttctc tattatccgg ccaatcctaa cagtgtacgg
      961 gaggcgaaag aggttctccc atccgcagcg cgcgcgctgg ggttgacgat tcagccttgg
     1021 gaagtacgcg atgcaaacgg cttcgataag gtattcgccg cattcaacaa gcagcggccg
     1081 gatggactct acgtccccgg gggaacgctc gtgacgacta accaaaagcg gattgttagc
     1141 ttggcgtcaa agagcctgtt accggcgatg tacaacagca gagacgctgt agaggctggc
     1201 gggcttatgt actacggcgc ggatctctca gacagccacc gtcgcgtcgc ctactatgtg
     1261 gacaggattc taaagggagc caggcctgcc gacctaccgg tggagcagcc gtccaagttc
     1321 gagctgatca tcaacctcaa ggccgcaaac cagattggtc tgacaattcc aagccgcgtg
     1381 ctcgagcggg cgaataaagt gatcaaatga cagtgctgag taacgaattc tgaggactga
     1441 gattcagatg aggaagaacc aggactaata gctcagcagt cggttttggc ggggacaagc
     1501 gataacaaag atgctttgcc tctctctaac aattacgatg ctggttgtgg gggctatcgc
     1561 tgaggcgcag caacaggcaa agatacctaa gattggatgg ctcggaattc gtgccgctgc
     1621 gtcagacccg gcacgcgaat cattccgtcg agagttccgt gcactcggct atattgaggg
     1681 gaagaacatt gtgttcgaat atcgatccgc tgacgataaa atcgagcggc tccccgcgtt
     1741 ggccgatgaa ttggtccgtc ttaaagttga tgtgctgctc gcgtggctga cgcctgctgc
     1801 cgtagccgcg aagaacgcga cccgtactat tccgatcgtt tttaacggtg gctttgatcc
     1861 cgtcgcggct gggctggtcg acagcctggc gcggcctgga gggaacatca cagggtttag
     1921 tggcatcgcg ccgcttttgg ccggcaaacg actcgagttg ctcaaggaaa tcagtcccaa
     1981 gctctcccgt atcgcggtta tttgggatcc gcagaatccg ggctctgcac aacaatggaa
     2041 agaaagccaa ctcccggcac gagaactgga agtgcaactc cattcgatag aagtaagcag
     2101 cgtcgatgag ttcgaggctg cgttcaaagc ggcgattaaa tcgcgcagtg acgctctcgc
     2161 cctaatggcg agcccgtttt tctattctaa tcagaaacag ttcgtggaac tggcgaccaa
     2221 aaatcaactg ccaacgatct atcctcggga agaatttatc actgccggcg gcttgatctc
     2281 ctacggatcc gacgggaagg aatcctttaa gcgcgccgcg tcattagtcg acaagattct
     2341 aaaaggcgca aagcccgccg atcttcccgt cgagcagccg acgaagtttg agttggtgat
     2401 caacttgcag gcggcgaagc agatcggtct cacgattccg cagaaggtgc tggcgagagc
     2461 ggatcgggtg atcaagtgaa acaggaagtc cggctagagc ttgtagagcg gaaaacgaag
     2521 ggctcgggcg caattagtca gcctttcatc atagctggcg agggtgacgt actgtccttg
     2581 gctgcggaga aactccatcg aggccaaatg aagggcgtcc agagttcgta ccagtttggg
     2641 aaatggttcc aacgctcgcg cgagcacagg cgcagccagt tctatcaagg ccaagcgact
     2701 caacagcgcg cgcacttcgt cggcatgcga tcgcgacagc ttgcgcgcat gaatccgagt
     2761 ccagagctcg tactcgataa gccggctaga aatcagcggc tcttgccaga ggcgatcagg
     2821 cggcgcgcgg tcttccgcca agagatgagc gagcgcgacc gaagtatcga gataaatcaa
     2881 cgatcgctcc ggtcttgttc aagctccttt tgtagcttgc ggaaagatgt gacgggattg
     2941 cgcggcggag gagtggatcc tgcaagcgcc ggaggcgtga tccagccttt gcgaaccgct
     3001 tcggccagca aggcgtcggc cagcaccggg cttctcgtgg tttcgggggg aacgatttcc
     3061 gcaacgaccc gatcccggtc ggtgaccagc accgtctcgc cgccttcggc gagccgaaca
     3121 tactcgctga gtttattctt taagatcttt aaaccgaccg ccttcatacg aatcaatgta
     3181 gcttccggta gctaccgatg tcaaggacgg atgtttaacc cagcccgttg cgttggaacc
     3241 tgtctttttc ggttccgccg aagcgcggag aagatgatca attgaggcga gaaagtgatg
     3301 agtgcagagc ggcaagagca gagaaaaaca tgaagaagga actcactatt tttatttttt
     3361 gcaccatgct ctttgcgctt tgcttgtcgg cgttggcgca acgcgcacgc ctggctcagc
     3421 tcgccacaat ccaccgaata ccagccattt actatcacga agcatttacc
//
LOCUS       DASUVS010000476         4953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_430781, whole genome shotgun sequence.
ACCESSION   DASUVS010000476 DASUVS010000000
VERSION     DASUVS010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430781"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VF182_25025"
     CDS             <1..823
                     /locus_tag="VF182_25025"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6440406.1"
                     /translation="LPSHRNYAKRQLHPHLLCSPFAYRTYHKPLGYAGDYEMVNMILR
                     DPNEGSTLFSKIINAWFLSQTPAGAHRNRIGYLTELLKHETLRVQGNDKTAKIFNLGC
                     GPAQEIQNFLVQDELCNHAEFVLLDFNEETIQHTGRVLENLKTKYNRRTKIEVVKKSV
                     HHVLKQAHRPTPAYDMVYCAGIFDYLSDRICKQLMNIFYELLAPGGLLVATNVESSNP
                     SRNGMEYLLEWHLIYRTPVQLKSLIPDRAPTRSFTVKTDDTGINVFLEVRKPDGL"
     gene            813..3500
                     /locus_tag="VF182_25030"
     CDS             813..3500
                     /locus_tag="VF182_25030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6440407.1"
                     /translation="MASEVPTQHEKEFEQAFISRERQDRIRSGKVACALVMFLMPAGV
                     ILDLWVYPEQVREFFQLRLICSVLAAAWWTLHQTSFGLRHYRLLGVPIAILPGFFIAW
                     MISVTEGPASPYYAGLILVLLAVNAIVHWSTAESLLALAVLYLFYLVACARWPIAEEQ
                     GIFFNNIYFLVLTGIIVVIGNYLYNQLQFREFVLRFELSQNRRTLEQANQKLIELDRV
                     KSQFFANVSHELRTPLTLLLTPLQSLIQEHKTELASPTRDLLLIMQSNGMRLLKLIND
                     LLDLVRLESGKMEVNAEPVDIDRLVHGVANAVKTTAQDRAIEVKVAIDSSVGTIVGDS
                     DKLERILLNLLFNAIKFTPAGGSVEIAVVRAERELVLVISDTGAGISQEQLPFIFDRF
                     WQADTSAQRKNQGLGIGLALVRELVEIQGGQVTVTSQLGTGTRFTIRLPYDEIDRRST
                     HGAKETAQSNDNESSPFSPYSNSNANKLGGIDRQAELYPSMALLRASLRPVGHDLDSQ
                     KPRVLIADDEPDMLRYLKSQLSLNFQVIEAVDGKQAVEKASQFLPDVIVCDMMMPEKS
                     GLEVCREIRERTHTKSTPILMLTARADEETKLTALAAGANDFITKPFSTTELRVRLKN
                     LADTFYLQQKLARQNQMLESTIEQLKDTEVQLVHSEKLASLGRMSAGIIHEINNPLNY
                     AKTGLYVLRNTIESPSTDQKDASLEVLQDIQEGIDRIDRIVSDLRIFTHPNLTQIERV
                     QVSELVTSALRLLSHELHDKVIVQNEIGSNQTIWANRNQITHVLVNLLQNAIYALGKK
                     TNAENPPTIWLRASEDYENNLLVIRDNGEGILPEILPKIFDPFFTSKDVGEGMGLGLS
                     ICYRIMQQHGGSITVRSERGVYSEFTLRFPRAANGMAAA"
     gene            3511..4770
                     /locus_tag="VF182_25035"
     CDS             3511..4770
                     /locus_tag="VF182_25035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX6440408.1"
                     /translation="MESAYDYRKCTVLYVDDEEMALKYFARAFDNRFPILTAVNAVDG
                     YRLLEQNRERIGLVITDQRMPGEKGVQFLERARQLHPRAIRILTTAYSDLHVAIEAVN
                     SGAIYKYVTKPWDIPQLEVTLRRAAEFFLVQRERDLLLKEKLSVLQRMMITDRVLTLG
                     ILAGRLGHYLRNSLVAVQTFIDLAPEKLLEEKISAEQIRNPSFWKEFYEQAQIQMRRI
                     TELLTDLVSASETSGSPPLEQVDLKQTVIRSLQRFNGSPLHQGITFVNQIPEGLPLIS
                     AEQGQLARLFELLIKDEIMSLPAGSHVFLNATPCRETQDIEIEIRDDGPGLPNEAFLS
                     VFDPFFFRNNNSQEFGINLMACYFIVYHHGGKITVQNQPGQGVTFTLTFPLQPKKVSP
                     AQEEEAFISKVLMNERFWERLISDQIV"
BASE COUNT         1263 a         1258 c         1230 g         1202 t
ORIGIN      
        1 tttaccgagc catagaaact atgctaagcg gcagcttcat ccccatctac tatgctcccc
       61 cttcgcctac cgcacctacc acaagccctt aggttatgcc ggcgattatg agatggtgaa
      121 catgatcctg agagatccaa atgaaggaag cactctgttt tctaagatca tcaacgcttg
      181 gttccttagt caaactccgg cgggagcaca ccgcaatcgt attggttatc tcacggagct
      241 tttgaaacat gaaaccctac gggtgcaggg taacgataaa acggccaaaa ttttcaatct
      301 tgggtgtgga ccagcccaag aaattcagaa tttcctcgtt caggatgaat tatgcaatca
      361 cgcggaattt gtcctgctcg actttaatga agaaactatt cagcacacag gcagggttct
      421 tgaaaatttg aagactaaat acaaccgtag aaccaagatc gaggttgtaa agaagtccgt
      481 tcaccacgtc ctcaaacaag cgcatcgacc gacacctgcc tacgatatgg tgtattgcgc
      541 aggaattttt gactatctct cggatagaat atgcaagcag cttatgaata tcttttatga
      601 gctgcttgca cctggaggac tgctcgttgc caccaatgtg gaatcttcaa acccgtcacg
      661 aaacggcatg gagtatcttc tcgaatggca tcttatctat cgcactccgg ttcagcttaa
      721 aagcttaatc ccggaccgag cccccacgcg aagtttcact gttaagaccg atgataccgg
      781 gatcaatgtt tttctcgagg taagaaagcc cgatggcctc tgaggttccg acgcagcacg
      841 aaaaggagtt tgagcaggcg tttatctcgc gggaacgtca agaccgcatc cgcagcggaa
      901 aagtcgcctg cgccttagta atgtttctta tgcccgccgg agtcatcctc gacctgtggg
      961 tctatccgga gcaagtgcgt gaatttttcc agctccgcct catatgttca gtattggcgg
     1021 cggcttggtg gacgcttcac cagacctctt tcggcctgcg acattaccgt ttgctcggtg
     1081 ttccgattgc tatccttccg ggcttcttta tcgcttggat gatctcagtc accgaaggtc
     1141 cggcctcgcc ctattacgcc ggactcattc tcgtgcttct tgctgtcaac gcgatcgtcc
     1201 actggagtac cgccgaatct ctgctcgctc tggcggttct ttatcttttt tatctggtag
     1261 cttgtgcccg ctggccaatc gcggaagagc agggaatctt ttttaacaac atttatttcc
     1321 tggtgcttac cgggattatc gttgttatcg ggaactatct ctacaatcag ctgcaatttc
     1381 gcgagttcgt gctgcgtttc gagttgagcc aaaacaggcg aacacttgaa caagcgaatc
     1441 agaagcttat tgagttggac cgagtcaaaa gccaattttt cgcaaatgtg agccatgagt
     1501 tgcgtacgcc actcacgttg ttgttgacgc cgctgcaaag tctcattcag gagcataaaa
     1561 cggaattagc ttcgccgacc cgagacctgc tgctcatcat gcaaagcaac ggcatgcgat
     1621 tgttgaagct gatcaacgat ttgctggacc tagttcgcct ggagtccggc aaaatggagg
     1681 ttaatgccga gccggtcgac atcgatcggc ttgttcacgg tgttgccaat gcagtaaaaa
     1741 ccactgccca ggatcgcgcc atcgaggtta aagtcgctat cgactctagc gtcggcacga
     1801 tagtcggcga cagcgataaa ctggagagga tcctgctcaa tctgctgttt aatgcaatca
     1861 aattcacgcc agccggtgga agcgttgaaa tcgccgttgt gcgcgccgaa cgcgaattag
     1921 tgctggtcat ttccgacacc ggcgccggca tcagccagga acagctgccg tttattttcg
     1981 accgcttctg gcaagccgat acctcggctc aacgaaagaa ccagggctta ggtatcggac
     2041 tcgcactggt gcgagaacta gttgaaattc aaggcggcca ggtgactgtc acgagccaac
     2101 ttggcacagg aacgcgattt acgatccggt tgccctatga cgagatagat cgccgatcca
     2161 cccacggtgc aaaggaaact gctcaatcca acgacaacga atcgtcccca ttttcaccgt
     2221 actcgaatag caatgccaac aagctggggg gaatcgatcg acaggccgag ctttacccgt
     2281 ccatggcact gctcagagca agccttagac cggtggggca cgatcttgac agccaaaaac
     2341 cgcgcgttct gattgccgat gatgaaccgg acatgttgcg gtatttgaag tcacaactga
     2401 gcctaaactt ccaagtaatc gaggccgtgg acgggaaaca ggccgtcgag aaagcgagtc
     2461 aatttttacc ggatgtgatc gtctgcgaca tgatgatgcc tgaaaagagc gggcttgagg
     2521 tttgccgcga gatcagggag cgcactcaca cgaaatcaac tcccattctg atgctcactg
     2581 cccgcgccga cgaggaaact aagcttacgg cactagcggc aggagcaaac gatttcatca
     2641 ccaagccgtt ttccactacc gagttacggg ttcgactgaa gaacctggct gacacgtttt
     2701 acttgcaaca gaaattagcg cggcaaaatc aaatgctgga atcgaccatc gaacaattaa
     2761 aagatactga ggtacaactg gttcattcag agaagttagc ttcgctcggt cgaatgagcg
     2821 ccggtattat ccatgaaatc aataatccgc tcaattatgc aaaaaccggc ctctatgtac
     2881 ttcgcaatac gatcgagtcg ccaagtaccg atcagaagga cgcttcatta gaggtgctcc
     2941 aagatataca agaaggaatc gatcgaatcg acaggatcgt atcagatctg cgcattttca
     3001 ctcatccgaa cctgacgcag atcgaaaggg ttcaagtttc tgagctggtg acatctgcct
     3061 tacgtttgct aagccacgaa ctacacgaca aggtcatagt tcagaacgag atcggcagta
     3121 accagacgat ctgggccaat cgcaaccaaa ttacccacgt gctggtaaat ctgcttcaaa
     3181 acgctatcta tgcgttggga aagaagacga atgctgaaaa tccgccgact atttggctca
     3241 gagcttccga agactacgag aacaatctac tggttattcg ggacaacggc gagggaattt
     3301 tgccggagat ccttccgaag attttcgatc cattcttcac aagtaaggat gtcggggaag
     3361 gtatgggact tggtctgagc atctgctatc gtatcatgca gcagcacgga ggatccatca
     3421 ctgttcggag cgagcgcggt gtctactcag agtttaccct gcgctttcca cgtgcggcaa
     3481 atggcatggc agctgcgtaa tggagagtct atggaatcgg cttacgatta tcggaagtgt
     3541 accgttcttt atgtcgacga tgaggagatg gctctaaaat attttgcccg cgccttcgac
     3601 aacagatttc caattctaac ggcggttaat gctgttgatg gttacaggct tctggaacaa
     3661 aatcgcgagc gaatcggcct tgtcattacg gatcaacgaa tgcctgggga aaaaggtgtg
     3721 cagtttttag aacgggcccg gcagctgcac cctagggcga tacgtatttt aactacggct
     3781 tactcggacc tacatgtagc tatcgaagcg gttaactcag gcgctatcta caaatatgta
     3841 acgaaacctt gggatatacc ccaactcgag gtaaccctca gacgcgcggc agagttcttc
     3901 ttggtccagc gggagcgtga tctgcttctc aaagagaaat tgtccgtgct gcagcggatg
     3961 atgattaccg atcgagtgct tactctggga attttggccg gccgactagg gcattatttg
     4021 cgcaactctc ttgtagccgt gcagaccttc attgatcttg cgccggaaaa acttctcgag
     4081 gaaaagatta gcgcggaaca gattcgcaac ccaagtttct ggaaggaatt ctatgaacaa
     4141 gctcagatcc agatgcgccg cataactgag ctcctgaccg atctggtaag cgccagcgaa
     4201 accagcggct cgccgcctct ggaacaggtt gacctgaagc aaactgtcat acgaagtttg
     4261 cagcggttca atggtagccc gctgcatcaa gggattactt ttgtaaatca aattccggaa
     4321 gggctccctc taatttccgc tgaacaagga cagcttgccc gcctttttga actattgata
     4381 aaagacgaga tcatgagctt acccgcgggc agccatgttt tccttaatgc gactccttgt
     4441 agggaaactc aggatataga gatcgagatc agggatgatg gtcccggcct gcccaacgaa
     4501 gcctttcttt ccgtattcga cccgtttttc ttccgcaaca acaattccca agaattcggt
     4561 atcaatttga tggcgtgcta tttcatcgtt tatcatcatg gcggaaagat cacggtccag
     4621 aaccaacctg gtcagggcgt caccttcacg ttgacctttc ccctgcaacc taaaaaggtt
     4681 tcacccgcgc aagaggaaga ggcattcatc tccaaggtac tgatgaacga gcggttttgg
     4741 gaacgtctga tttcggatca gatcgtctga ggcacggctt tgataaacaa cgtttctgtt
     4801 gactgctcct cgatcggctg ctcgacttag gctcgctgat taaactccgc cgcaccggga
     4861 gcttccggaa gaactagtcc tgggcaagat tgggtttaac taagttgttg ggcgctaaaa
     4921 tggagtgact atacccgttc ttgccaaagc gcg
//
LOCUS       DASUVS010000477         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_868464, whole genome shotgun sequence.
ACCESSION   DASUVS010000477 DASUVS010000000
VERSION     DASUVS010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868464"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(360..679)
                     /locus_tag="VF182_25040"
                     /pseudo
     CDS             complement(360..679)
                     /locus_tag="VF182_25040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010364340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
     gene            complement(735..923)
                     /locus_tag="VF182_25045"
     CDS             complement(735..923)
                     /locus_tag="VF182_25045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440409.1"
                     /translation="MKTNLKRKKYYLDENKNSPVKDLGVKTETEAIDAALNLVVFRKE
                     ILKSLDKVAGKGGVQKVL"
     gene            1101..2384
                     /locus_tag="VF182_25050"
     CDS             1101..2384
                     /locus_tag="VF182_25050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX6440410.1"
                     /translation="MADLFRLTASEAAARIGDGKLTSEALVRSCLERIDAREAQVKAW
                     VHLDRDFALAQARECDKSGSRGPLHGIPFAAKDIMDSADLPTEYGSPIYKGNRPKADA
                     ACVALSRAAGGVLLGKTVTTEFASRFPFGKTTNPHNPEHTPGGSSSGSAAAIGDFMVP
                     LGFGTQTVGSVIRPAAFCGCIGYKPSFGEFSTQGVKQNTASFDTVGLFARAVEDLALF
                     RAALTGFPTKSLADVPVNNLKIGFCRLFWDRAEGYTKKFLEAAASALAKAGAKVSDVD
                     LGKAFEQFETMGRRINDFEFSRGLTWERQNHWDLLSQFQRDKFAGWLNISYEQYREAE
                     EALEKCRNHLADTMKDLDLLLTPSAPGEAPAGLASTGDTSFNVLSTWTYTPCLTLPVF
                     TGPSGLPVGIQLIGHRNQDHHLLEGAQAVYRLFAK"
     gene            2526..>2726
                     /locus_tag="VF182_25055"
     CDS             2526..>2726
                     /locus_tag="VF182_25055"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6440411.1"
                     /translation="MQNVDPDIIVIGAGGCGLMAALVAAKKGARVLLLEKTDKPGGGT
                     AFSSKGIRAAGSRRQRELNVEDN"
BASE COUNT          575 a          787 c          729 g          635 t
ORIGIN      
        1 cggcgcgcct tgcggaatcc gatcgcccca cttgggatcc atatatctca gatacagatc
       61 atgcggctcg tacacatgca tgtcgctgtc gagaattttg taaccgcttc gtgccatgat
      121 cattcccttc tagaaatcac ccgggtataa gaaatctatg tgatcgctat ctcaaatccc
      181 cgtccgcgcc cgatcatcgt tcttatgact gcgtcaatct attgttgctt agattgattg
      241 cgtgataaca aatgttcgcc taccaaaacc tcggcccatc cgtgatataa gtgccatcta
      301 tcgcggttcc tgctcctcac gcaagcgtaa actgcctgtt tcctctgtcc attcccgtgt
      361 catgccagca ccctccgatt gtcttcgatc ctctttctca ccgtcgcagg cagccgcttc
      421 tcagcggtac gcaagtcata ggtcatttgc aggttcatcc agaattccgc cgacgttttg
      481 aaaaacgttc ctagccggat tgcggtgtcc cccgtgatgc ctctttgatc cttcagaatc
      541 gccgtaatcc gattggcggg cacccgatag ccttcgcaag cgcgttggcc gtcaggccca
      601 acggtttcaa aaattcctcg tcgagaattt caccctggga atcggtcgca ttccgttctt
      661 tttcattttg tactcctatg attaaccgat cacgatcaat gaaaaagacc gttggcgctg
      721 caggtgcttg gccgctaaag aaccttctgt acaccgccct tgccagcaac tttatccaat
      781 gatttcagaa tttctttcct gaaaacaacc aaatttagtg cggcatcaat cgcctcggtc
      841 tcggtcttca cacccaggtc cttaaccggc gaattcttat tctcatcaag ataatacttt
      901 tttcgtttga gattggtttt cataaacctc catacatacg actccctatt ttatacgtat
      961 gcgggtggac ttccagtcgc agtctactct gacgacgact gcgcttattg cagtcaccac
     1021 gcctgctcgg ttatgttcgg aaaagtttcg gattcatcgt agagttgaat tcctctttac
     1081 aatctttgaa ggagaatcac atggcagatt tatttcgctt aacggcgagt gaagcggcgg
     1141 cgcgtatcgg cgacggcaaa ctcacctccg aagctctggt gcgctcttgc ctcgagcgaa
     1201 tcgatgcacg ggaggcgcaa gtgaaagcgt gggtccatct ggaccgcgat ttcgcgctgg
     1261 cacaggcgcg ggagtgcgac aagagcggaa gccgaggtcc gctccacgga attccgttcg
     1321 ctgcaaaaga catcatggat agcgccgatc tgcccaccga gtatggatct cccatctaca
     1381 aaggcaatcg tccgaaggcg gacgcggctt gcgtcgcgct cagccgcgcg gccggcgggg
     1441 tgcttttggg caagacggta acaacagagt ttgccagtcg atttcctttt ggcaagacga
     1501 ccaatccgca taatcccgag catacgcccg gcggttcctc gagcggctcg gcggccgcga
     1561 tcggcgactt catggtgccg ctggggttcg gcactcagac ggtcggctcg gtgatccgtc
     1621 cggcggcgtt ctgcggctgc atcggataca agccgagctt cggcgaattc agcacgcagg
     1681 gcgtgaagca gaataccgcg tcgttcgaca ccgttggatt gttcgctcgc gctgtcgaag
     1741 atctcgcgct gttccgagcc gcgctgacag gatttccgac gaaatctttg gccgacgttc
     1801 cggtgaataa tctcaagatc ggcttttgcc gattgttctg ggaccgcgcg gagggataca
     1861 cgaagaaatt ccttgaagcg gccgcaagcg cgctggccaa ggccggagcg aaagtctccg
     1921 acgtcgatct cggcaaggcg ttcgaacagt tcgagaccat gggccggcgc atcaatgatt
     1981 tcgagttctc gcgcggcctt acctgggaac ggcagaacca ttgggatctc ctgagccagt
     2041 tccagcgcga caagttcgcc ggctggctca atatctccta cgaacaatac cgcgaagccg
     2101 aggaggcgct ggagaagtgc cgcaatcatc tcgccgatac tatgaaagat ttagacctgc
     2161 tgctcacacc gagcgcgccc ggcgaggctc cggcgggact ggccagcacg ggcgacacgt
     2221 ctttcaatgt tctatcgacg tggacctata ctccctgcct caccttgccc gtgtttacgg
     2281 gaccatccgg attgccagtg ggcattcagc tcatcggcca ccgcaaccag gatcaccatc
     2341 tgttggaagg agctcaggct gtgtaccggc tatttgcgaa atgaaaatga tttccacgtc
     2401 gtcaagcctg gaccctcagc ttagccgtct gtcggaagtg cgaggagctt caagattacg
     2461 ggcatgccgc caccgcatgc tccctgaata gacggcttca ttccaatcgg attctcggag
     2521 gctccatgca gaacgtagat ccagatatca tcgtcatcgg cgccggtgga tgcgggctca
     2581 tggcggcgct ggtggcagcg aagaaggggg cgcgggtgct gttgctcgag aaaacggata
     2641 agcccggcgg cgggacagcg ttttcgtcca agggcattcg cgccgccgga agccgccgac
     2701 agcgtgagtt gaacgtcgaa gataac
//
LOCUS       DASUVS010000478        27355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_211827, whole genome shotgun sequence.
ACCESSION   DASUVS010000478 DASUVS010000000
VERSION     DASUVS010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 27355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27355
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211827"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VF182_25060"
     CDS             complement(<1..524)
                     /locus_tag="VF182_25060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI"
                     /protein_id="HEX6440412.1"
                     /translation="MARKNTKSHSDVVEKKLVGVADSVITAAQRSQDPTLSIPVRSLA
                     NVNFNEKRGIIEMGRRKQARSFFNVGMAKKFMQTVLVADALAELQRANLTTSLREIYY
                     RTKHTIKNSHENTFDGQNESDPLIEDLEVTLEALREELHVRAENSGTVVGPLVLVDDG
                     DRVDCTRLGKGGYSV"
     gene            complement(533..2554)
                     /locus_tag="VF182_25065"
     CDS             complement(533..2554)
                     /locus_tag="VF182_25065"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HEX6440413.1"
                     /translation="MSKAQAKSTDLIEVPPAAPKILLSAVEMGARQREISVSEFFTKN
                     RHLLGFDNPRKALLTCVKEAVDNALDACEEAGILPEVIVKLEVAANGDAAPPPSQANR
                     FRITVIDYGPGIVRQQIPKIFAKLLYGSKFHRLRMSRGQQGIGISAAGMYAQLTTGKP
                     VQITSRISAKSPAHYFEVQIDTKKNEPRILENKKIDWENHRGTQVTLEIEGRYQKGRA
                     SVDEYLEQTAIANPHVKLVYQTPEGETKEYPRTIEELPPQPREIKPHPYGIEFGILLR
                     MLHDTKSQSVGGFLSSDFSRVSSNLAQEICKAAKLSPGIRPRNVHGSAAEALYKAIQS
                     TKIMAPPSNCISPIGERAILHGLYKQIKGEFYTAVTRPPSVYRGNPFLIEAGLAYGKA
                     PEQAPEKPEQPRVALAEGEQQDNDSELARVIRYANRVPLLYQQSACSTFKAVLDTTWR
                     NYGVPQSRGALPAGPMVIFIHMASVWVPFTSESKEAIADYDEIRKEIKLALQECGRRL
                     GVFLRRREHAKSEFRRRNIFELYIEEVVEACNRLKGGKLPKEKLKEQLQKIAAKRTGG
                     QKTDEALGRNVGPEGLPFSIIVTPEGTEGDAPVIAGDDRSELAPPEEAREDGPLAGAV
                     ELDATTESFTKIKKKTAQTKQQISSKVESRSKAKAAAKKFRTRSKRSKK"
     gene            complement(2903..3907)
                     /locus_tag="VF182_25070"
     CDS             complement(2903..3907)
                     /locus_tag="VF182_25070"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6440414.1"
                     /translation="MNTIDADGHIVEKDADVRKHLPEPHSKRGRPLLPSDGMDTGLGG
                     LVGGLEDCDVATRLKDMDKEGIDTSVLFPTSSFAVNGLIERNYAVAYARAYNDFIGEV
                     CRQSPRLKGVALLPFQHPAAAVEEANRAVTKLGLSAIAVATQGMKEHLGSQTFWPIYD
                     ELQRLNVPLCVHNRRAGPAGEIRFDSFMFMHTIGRPVETVIQFAGLIYGGVPEHFSKL
                     RIAFLECGVGWVPYWMERMDEEWEKRGKVEAPACKQKPSEYVKHGNLFFATEPEEETL
                     PYVLERIGDDKVLFASDYPHWDGMFPYVVSTIRGRKDISGEAKKRILGENAKRFYGWQ
                     "
     gene            complement(3950..4240)
                     /locus_tag="VF182_25075"
     CDS             complement(3950..4240)
                     /locus_tag="VF182_25075"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEX6440415.1"
                     /translation="MNTSAGRFKIGDAVVVATEMAHRNNPRTPTYVRGKRGVIGLVHG
                     ILENPKDHRGLYDPLYTVRFDLSAVSPCQDSDSVWVDVHEEWLMPLNDEGTD"
     gene            complement(4249..5322)
                     /locus_tag="VF182_25080"
     CDS             complement(4249..5322)
                     /locus_tag="VF182_25080"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEX6440416.1"
                     /translation="MSGKHGHKPFEGHMHDYEAHVKAVEEDLEYYRAKRLEPRIIYLI
                     RRGVVTFYDLLKARAALKRSNQFFKPRKAQGINVEARVRFLEEWLDEYVKGIALVSSR
                     SVEGMDMVIEDNRKERGDYDDFFRIKKKDHHGTLEERMLNLEQDLKDYHDLLEVFVQA
                     LIARKWATRQELEQRWQQLHEERPWAGSVIVAKAWTDPEFKQALLTTGREALREMGIH
                     QGKIGKLRVVENTDAVHNVVVCTLCSCYPYDILGDTPWWYKHDVYKERIVQTPRVVLK
                     EMFSLELAPNQEVRVWDSTSDVRYFVLPKRPAGTEGMSAEELATLVTVDSLIGAGLPM
                     EPSQWNEARIAGAAPETPRVRPD"
     gene            complement(5437..7155)
                     /locus_tag="VF182_25085"
     CDS             complement(5437..7155)
                     /locus_tag="VF182_25085"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014537808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HEX6440417.1"
                     /translation="MADPNKTGKKPRIAVFSGPTATIQNSEPLVTSNKARAKYGLSPR
                     NQPDGTPLRFDVLRPQRLAAPVTVYVEQFSAHPLERDSAELYAPPDGYVDAAGAFHRE
                     RTSAADKPVYEIMLTPQDGLYPLPYMARQGNGQPWELDGTEKDAPAELCRVPFFPDGS
                     RLFEEIDRLGVSDEGVGCLLSGKAEFDFYRPLPSGGYAKGRSAGERTDIGDGEIAPEI
                     RGTDFFPYRPPYLRQEPPMSALARVTNIVQGALNSGNYSGAIWLEGSPFVEETIYWLN
                     LLIDTTVPIVGNSSQRPHGAVGNDGDRNIVDSVDYISSRIWADESGKDCIGAVAILDE
                     QIFTSRDVQKADARPGGYVATGGHGGIIGRIGHPGMPQFSFKTIKRHTHNSLVNTAHL
                     PNQVQGSRMQGSEVVLIAVPIKEATGNLLPAAIPKVTLLKHARYLPENTAGGAEQEVD
                     ILARMDKNLRDAPLAGFIAEGSAPFGAMSNSVDAALKRATFSGMPVVKVGRGNASGFV
                     DAKRDPLSIAGNNLTATKARLLLMACLLKFGCLPPAVDAAKPSAAEVDATKDRLKQYQ
                     EIFDTH"
     gene            complement(7279..7872)
                     /locus_tag="VF182_25090"
     CDS             complement(7279..7872)
                     /locus_tag="VF182_25090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440418.1"
                     /translation="MTSSSQSAVERFITSARELFALENDPAERWKKMTPLLEALIGDR
                     SIKEQSKDWPACRQGDERAQNLLFYEDPEYKFVINGLIKSPKTRTQIHDHAHNWTLYG
                     VLDGHESIERYERVDDGAKSDYADIRQKEKVNVGPGKVDLVGPFQIHAEESGQERTVA
                     IIVRAEKAGGFLQGRYDPATKRYWQGYGPEQIPYELK"
     gene            complement(8015..9274)
                     /gene="ahcY"
                     /locus_tag="VF182_25095"
     CDS             complement(8015..9274)
                     /gene="ahcY"
                     /locus_tag="VF182_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX6440419.1"
                     /translation="MKYDIKDIGLAQNGRKRILWAGQDMPVLQRVRDRFQKEKPFQGM
                     RFSACLHVTAETANLVQVLKAGGADVLLCASNPLSTQDDVAASLVQHDRIPTYAIKGE
                     DNKSYYSHLRAALDHKPIITMDDGADLVSLLHTDYSHLASGLTASMEETTTGVIRLRA
                     LEKDGALKIPVIAVNDAATKHLFDNRYGTGQSTIDGVIRATDMLIAGKRVVVAGYGWC
                     GKGVAARARGMGAKVIVTEVDPVRALEAAMDGFDAMTMKEAARIGDLFITLTGDMHVI
                     SADHLKLMKDGAVICNSGHFDIEIDLGALKKLSTKVEKNIRNSVDAYTLPGGKRIFLL
                     GEGRLVNLAAAEGHPASVMDMSFATQALASEWAVKNSHNLKPQVYEVPKAVEDWVAKL
                     KLTSMGIKIDSLTKEQEKYLSSWSHGT"
     gene            complement(9313..10491)
                     /gene="metK"
                     /locus_tag="VF182_25100"
     CDS             complement(9313..10491)
                     /gene="metK"
                     /locus_tag="VF182_25100"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX6440420.1"
                     /translation="MAGRDFLFTSESVSEGHPDKLCDQISDAVLDAHLARDPDSRVAC
                     ESLAKTGMIVIAGEITSKAQVSYAEIAREVVREIGYTDSAMGFDGNTCAILTAVEQQS
                     PDISQGVTEGEGLHKEQGAGDQGMMFGYACDETPEFMPLPIQMAHSLVKYLAKIRKGG
                     EAYFLRPDSKSQVTVEYRGGKPVKVKSVVISSQHSPEVEYSKLRETIIDGVIKAVIPS
                     EYLSKDTVFHVNPTGRFVVGGPQGDCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVD
                     RSAAYMARYVAKNIVAAELAQRCEVQLAYCIGVAQPVSVLVDTFGTGTIAETTIAKIV
                     QEVFTLTPKGIIQTLDLKRPIYRSTASYGHFGRTPEDGHFTWEKTDRVEDLKKAAACA
                     "
     gene            complement(10559..10834)
                     /locus_tag="VF182_25105"
     CDS             complement(10559..10834)
                     /locus_tag="VF182_25105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018901147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX6440421.1"
                     /translation="MVKVVKKMEIKNKLGVHARAAALLVQTVNRFSAQVTISKDGQTT
                     DGRSIMGVLMLAATQGSTIEVEAAGRDAEQAVKALEKLVERRFNENE"
     gene            complement(10838..11704)
                     /gene="rapZ"
                     /locus_tag="VF182_25110"
     CDS             complement(10838..11704)
                     /gene="rapZ"
                     /locus_tag="VF182_25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX6440422.1"
                     /translation="MTDDLEIIIITGLSGSGKSVAIRALEDNGFFCIDNLPAPLIPKF
                     IDLCVGYSEGITRIALGVDLRAGQFLQSLPQVLTDMRNAGHHVQMLFFDASDEVLLRR
                     FSETRRPHPLAGEEPIQEGISRERKALEAMRELVDKVIDTSDLNVHELKREMEERFCQ
                     APYSRTMNVFLTSFGYKYGIPHDTDMILDVRFLPNPYFVSELRAHDGLKREVEEYVLK
                     NEETRTFLDRLYSLLEFTLPLYEREGKSSLTVALGCTGGRHRSVVLVEELHKRLAVGK
                     FRIHVKHRDIEK"
     gene            complement(11711..12172)
                     /locus_tag="VF182_25115"
     CDS             complement(11711..12172)
                     /locus_tag="VF182_25115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX6440423.1"
                     /translation="MKITDFLTLDRVIADLSGKEKSAVLEEMANQLADRPAGLDPAQV
                     LRVLQDRERISTTAIGEGVAIPHGKLPTVEKISGLFARSLQGVDFASLDGEPTFLFFT
                     LIAPQEAAADHLKALARISRLLKDPDLRHRLLAAGNSQDLYDIIATEDQKL"
     gene            complement(12169..12489)
                     /gene="raiA"
                     /locus_tag="VF182_25120"
     CDS             complement(12169..12489)
                     /gene="raiA"
                     /locus_tag="VF182_25120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX6440424.1"
                     /translation="MSDVKVSVTFRHTESTDALKQYVEEKMHRLGKYFSQSLDAHVVL
                     SVESKERQTAEVELHAHGGMIHAKEQTEDLYAAIDLVTDNLKRQLRKQKDKIKLEPRR
                     KPPE"
     gene            complement(12512..13981)
                     /gene="rpoN"
                     /locus_tag="VF182_25125"
     CDS             complement(12512..13981)
                     /gene="rpoN"
                     /locus_tag="VF182_25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX6440425.1"
                     /translation="MALEIRQVQKLAQQLVMTPQLQQAIKLLQLSRVELEDMVTKELQ
                     ENPALEEGPSEESESGDQPRSENTGETGQTEPVVNRELSAVDKIGTYDWQEYLDTHSN
                     SMHGSLTAEAGSDDGDAPPSWENSLTKKTSLEDHLLWQLRLSKISERESMIGQYIIQN
                     LSENGYLALTLEEVCAATQTDPEEAEKVLKRIQFFDPVGVAARNLRECLLVQLQNLEL
                     GDSLAARIVSDYLSFLESKRYEKLARDLGTTIDEIADAAHLITSLEPKPSRGFEQEDV
                     RTVLPDVFVEKIAGEYVIYLNDDGVPRLRVSPLYRRMVGQEGVAEEQARQYLQEKVRA
                     ATWLIKSIQQRQQTLYRVTQSIFKFQLDFLDHGVSQLRPMVLRDVAEDIHMHESTVSR
                     ATANKYVHTPQGLFELKFFFQSGLRGGNGEDVASESVKEKIRGIISGEDPRKPFSDQH
                     IAALLSDCSIDIARRTVAKYREAMGILPSSKRRQPFFRK"
     gene            complement(13983..14705)
                     /gene="lptB"
                     /locus_tag="VF182_25130"
     CDS             complement(13983..14705)
                     /gene="lptB"
                     /locus_tag="VF182_25130"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX6440426.1"
                     /translation="MSVLRAEAVAKSYNGRRVVNGINLDVQGGEVVGLLGPNGAGKTT
                     TFHMMVGLVHPDQGKVLLNDKDLTAAPIYQRARAGISYLPQESSVFRRLTVEDNLQAI
                     LQTLDLNSVERQERCETLLNMLGVGHLSKNKAFTLSGGERRRVEIARALVLSPQFVLL
                     DEPFTGVDPIAVAEIQKIIRELITSGIGILITDHNVRETLGICDRAYILNEGAILEQG
                     TPEVISESPKARKFYLGEGFKF"
     gene            complement(14702..15283)
                     /gene="lptA"
                     /locus_tag="VF182_25135"
     CDS             complement(14702..15283)
                     /gene="lptA"
                     /locus_tag="VF182_25135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEX6440427.1"
                     /translation="MTMKFPSLGFALAVFLLAGFIVGPAGGASKEDGKSKSDESVFEF
                     NKKEPIYITSDWMEVDQKKNTITYKGRVVTIQSEMTMRSETLTAVFDPEMKQMKQIIA
                     EGKVNAVQGNRMATGDKAIFDDKAKTVTLTGNPVMRQGNSQVTGSRIIYYIDQDRAVA
                     EGKDSVRVQVTIFPEELQKKDNAKSEAGSSTAK"
     gene            complement(15289..15900)
                     /gene="lptC"
                     /locus_tag="VF182_25140"
     CDS             complement(15289..15900)
                     /gene="lptC"
                     /locus_tag="VF182_25140"
                     /inference="COORDINATES: protein motif:HMM:NF018537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX6440428.1"
                     /translation="MRKKRLLLLVAIIASLGAVGYKVTAEIWANKLRDIRQNPIKLLD
                     ELPDSALNIKDFRRAKVENGRTVWELLGEEASYDKDQRQAVIHKPRFLYYDKNGEVAE
                     ASANIAHVYLNEKELEKMQLEGGIEISYSGYLLNSQEATYLPAKEQIVMPQRTTLTGE
                     GLELQGASMEVDLGESKVRLVRDVKTKLEPEKLAKQKAKSEKS"
     gene            complement(15994..16527)
                     /locus_tag="VF182_25145"
     CDS             complement(15994..16527)
                     /locus_tag="VF182_25145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEX6440429.1"
                     /translation="MKRISTAVRNKAKAIKLLLLDVDGVLTDGRIILDDRGVETKHFD
                     VRDGQGIVLLMGAGIEVGLISARSSKAVRYRAKELDLQIVHQGTQNKGRTYAEIRRQS
                     GLAETEIAYVGDDIADLSILHRVGLAVTVNDACQELFRSVDYVTEKPGGRGAVREIAE
                     LLLKAQNKWGFIVKKIS"
     gene            complement(16536..17504)
                     /locus_tag="VF182_25150"
     CDS             complement(16536..17504)
                     /locus_tag="VF182_25150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX6440430.1"
                     /translation="MNQKAILGRAREVLNIEIQGLLSLVDRLDEHFCSAIKLLRGCNG
                     KVVVTGMGKSGLICRKIAATLASTGTPAFFLHSSDALHGDLGMVMKGDVILAVSNSGE
                     TEEILKIIPHFKRHALKMIVMTGNPESTLAKAADVILDVSVREEACPMGLAPTASTTA
                     TLAMGDALAVVLLETKGFNQEDFAMRHPGGILGRRLFLRVEDLMHRGDKLPLVSEATP
                     IKDALFEITSKRLGITGVVDGEGKLTGVITDGDLRRGLESKGDIFEYNAKQLMTRNAK
                     SIGADCLAAEAVAAMEQHSITCLFVLHENGTPMGAIHLHDLLKAGL"
     gene            complement(17501..18331)
                     /gene="kdsA"
                     /locus_tag="VF182_25155"
     CDS             complement(17501..18331)
                     /gene="kdsA"
                     /locus_tag="VF182_25155"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX6440431.1"
                     /translation="MKPVQIGNFKIGAGQPLAVIAGPCVIESQESALRHASLLKQAAD
                     RAGVPYIFKSSYDKANRSSGDSFRGPGLEQGLEILAEVKRKVGVPILTDVHESAQVGP
                     VQQIADVLQIPAFLCRQTDFVIAVAKSGKVVNIKKGQFLAPWDIGNVVEKILSTGNDR
                     VLLTERGVSFGYNNLVSDMRSLVWMRELGYPVIFDATHSLQLPGGLGHASGGERKYIP
                     ALARAAVAVGIDALFMEVHEEPDQALSDGPNSLALKEFEKLIRAVKQLDELVKGSSIS
                     "
     gene            complement(18360..20006)
                     /locus_tag="VF182_25160"
     CDS             complement(18360..20006)
                     /locus_tag="VF182_25160"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX6440432.1"
                     /translation="MATVKTKFIFVTGGVVSSLGKGLASASIGALLESRGLKVTMLKM
                     DPYINVDPGTMSPFQHGEVFVTDDGAEADLDLGHYERYVSTLMGRKNNCTTGQIYETV
                     IAKERRGDYLGGTVQVIPHITDEIKRRILAAADGFDIAIGEIGGTVGDIEGLPFLEAV
                     RQFAWDWGRENVLYIHLTLVPFITGGGELKTKPTQHSVKELTGLGIQPDILLCRTDRF
                     LDPKIKSKIARFCNVAENSVITAKDVESIYEVPLVFHEEGLDERIVEKLNIWTGAPNL
                     KKWQHVADVLNNSKDSVRIAVVGKYMSLKESYKSLLEALVHGGIANEARVDLDCIEAE
                     KIEQEGVQAVLKDVDGLLIPGGFGDRGSEGKIAAIRYARENQIPFFGICLGMQMAVVE
                     FARNVCGLDGANSSEFDLQTPHPVIHLMEEQKSIDTKGGTMRLGNYPCVLQNGTLAAK
                     LYGREKISERHRHRYEFNNTYRAEFGAKGMVLSGLSPDSNLVEIIELKNQPWFLGCQF
                     HPEFKSHPMDCHPLFRGFIQAALEKRQALSELPRMKVVRT"
     gene            complement(20131..20904)
                     /gene="kdsB"
                     /locus_tag="VF182_25165"
     CDS             complement(20131..20904)
                     /gene="kdsB"
                     /locus_tag="VF182_25165"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX6440433.1"
                     /translation="MSVVAIIPARYGSTRLPGKPLAWIGGKPMIQHVYESTSRATELD
                     RVVVATDDLRIEEAVRKFGGEVIMTARDHPSGTDRLAEVARKVRADLLVNVQGDLPFI
                     QAETITRAVRPMKRDPSIPMGTVCTAILDQDEWLNPNIVKVVIDRKGFALYFSRAAIP
                     YSRNDAIDLNGGKVRRSVKRRLWGYRHLGLYVYQREFLLKFARLRPTALEQIESLEQL
                     RALAYGYRIRVVQVDERSVEVDTPEDLQRAQRYLKGRNR"
     gene            21188..22120
                     /locus_tag="VF182_25170"
     CDS             21188..22120
                     /locus_tag="VF182_25170"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440434.1"
                     /translation="MGNLIKIDAGFATIGQGAGPMMVTWKAGLFNKHGLEVDKPRIMG
                     GAKGVVRGLMAGEIQFGNLAAPAPLRSNVKGEADLIFLTGGINQQFIMGRPGIASREQ
                     LTGKKMGFVGDGGLNDALVNFIIEKLSDAGIKGIDKEPIPAGGAEQMAALIGGKCDAM
                     VITPPESIDARRKGCNYLIDLAEYGLNYALGGIAARRDYVEKNPEITHKFIKAYVEGM
                     HRYRIDRDFTVEVQAEYSGIADRTVAEETYDLTRPGMPPIPYPVVASLQVLLDFMSKE
                     IPEAKNVDARRFVDDHFIRELEENGFIASLAGVK"
     gene            22187..22957
                     /locus_tag="VF182_25175"
     CDS             22187..22957
                     /locus_tag="VF182_25175"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX6440435.1"
                     /translation="MRVMFLNHSFKRHSAELETHIERLLKSYASPETTFELAYPEDLG
                     GGEVLSLLEQGKALSGLHHILETPALVKKAIEAEQLGFDAVIQSNTFDPGVEASRLAV
                     RIPVIGLLRASLHFAATICDKFGLIVPLPAHMPHTMRLVQSYGMNRFVTGIKTVDLYD
                     TGDLSGYHDLVIERTLVMGRELIEQGAQAIIPLGGKIYPYVVTPEELAPNLGVPIINT
                     KAVGVSYAELMARSRITHSQRAYPWSPGLNPDKVSMRR"
     gene            23111..24205
                     /locus_tag="VF182_25180"
     CDS             23111..24205
                     /locus_tag="VF182_25180"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6440436.1"
                     /translation="MIIDADGHFFETEEIFDRYMEPDLRNYRPRLLSDEDGYNYWVVD
                     GQTSYRRPSVKGAGAPGTAAPPGKKIQAARRASAGSQTLTNIRERLDDLDKEGIDIQY
                     LYPSFLLHINALADGILAMGVCRAYNSWLAEACRKAADRLKGIGVVSLQDSQGAAKEL
                     SRIKDLGMTAVMINGTAGSKRLDHPDHDPFFAEADRLNLPIAVHFSLQFPTVDKLFEH
                     HFPNRVLAGIFPIMAGLTSVLCSGLLDRFKNIKFAFLEGGISWVPAHVERMDDHFENP
                     RYGARDLISHPPSEYLKSGRIFFGCEGNEPFLPRVIADLGEELFMFSSDYPHADRTEG
                     TAGYLKTRSDIGMNVRHKLLADNAQRFYGK"
     gene            24471..24764
                     /locus_tag="VF182_25185"
     CDS             24471..24764
                     /locus_tag="VF182_25185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440437.1"
                     /translation="MEKSTKAKSGTATGEVTSVDAKSGQLTVKAKDKDLNLMAESKSA
                     KNALDKIKVGDNVRVTYTDKDGKMVVSSVKADSKGMSKSEERKSSTRAEKKQP"
     gene            24954..25136
                     /locus_tag="VF182_25190"
     CDS             24954..25136
                     /locus_tag="VF182_25190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440438.1"
                     /translation="MDLPKCQKCNNGILIPLSDYGQDGASVMFKAWACTNPDCGFSLR
                     VDKGEVTYGKRIEPKH"
     gene            complement(25176..25832)
                     /locus_tag="VF182_25195"
     CDS             complement(25176..25832)
                     /locus_tag="VF182_25195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440439.1"
                     /translation="MEALKAEEFLTGLVNYREAFWEKMNAERKSYGAEKPLTPAIVLR
                     RLQFGVVMERKAAEVTARWVGDIPDLDLQQPMSEYVVNELKHTSILRKRIGELNGDPD
                     ALWNQPLKELKELWDYHASLGSLCELIASVQFGHEEFFPRTSKSFIERVQSIDPQTAS
                     VYRDTLLADEAGHEWIAPEILRRYATDGETQKRCLEALKIGCELFGKAIQSFYQSLPA
                     "
     gene            25974..26729
                     /locus_tag="VF182_25200"
     CDS             25974..26729
                     /locus_tag="VF182_25200"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6440440.1"
                     /translation="MESQEAKNLVDNLYKELYEHWDRKINQGPFMSRLRDGTLPLPCL
                     RRFFKDWGLFSIEVVALNAVSYYVHLPFFVRNFDLLPAFSDKIAEELVAPKPPGHTLI
                     LLETANALGLSKEELFEQPASAPGRAISDYSRRVFQDGSIIELWGAHVYEETLGHWAQ
                     QWGEALVSHYGLSQRQAIYFTAHAEADLVEHEEHMGHGPLNRMILQRILEQGLTATKL
                     GYDPKYCGFTMVDLHALMERHALENPYSSENRS"
     gene            26806..>27355
                     /locus_tag="VF182_25205"
     CDS             26806..>27355
                     /locus_tag="VF182_25205"
                     /inference="COORDINATES: protein motif:HMM:NF025330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX6440441.1"
                     /translation="MIRAGAVDVGVLVFLLSWCLFSQAGAEPPGNWQIRAPLPSSRTE
                     VAAAGIAGKIYVIGGYGKNGNLVEAYDPQTDRWERRASLPKRLHHSGAAAVNGKLYII
                     GGYISGIGPIDTVYEYDPAADRWTAKKPMPIARGALAVGVIAGKIFAAGGVGPDGKNT
                     AAVQAYDPFQDRWTDHAPMPTPR"
BASE COUNT         6071 a         7632 c         6995 g         6657 t
ORIGIN      
        1 accgaatacc cgcccttgcc aagcctggtg cagtcgacgc gatcgccgtc atcgaccagc
       61 accagcggcc ccactaccgt tcccgagttc tcggcgcgca cgtggagctc ctcacgaagc
      121 gcttctagag tgacttcaag atcctcgatc aacgggtcgg attcgttctg cccatcgaat
      181 gtattttcgt gcgagttctt gatcgtatgt ttggtgcggt aatagatttc ccgaagcgac
      241 gtggtcaggt tggctcgctg cagctcggcc aaggcatcgg caacgagcac tgtctgcatg
      301 aattttttcg ccatcccgac attgaagaac gagcgcgcct gcttgcgccg gcccatttcg
      361 ataatcccgc gcttctcatt gaaattgacg ttggcgagac tgcgcacggg tattgaaagg
      421 gtcggatctt gactgcgttg cgccgccgta atcaccgagt cagccacccc aaccagcttc
      481 ttctctacaa cgtcgctatg actttttgtg tttttacgcg ccatgttttt tcctactttt
      541 tgctgcgctt cgatctagtc cggaactttt tagcggcggc tttagctttg ctacggcttt
      601 ccactttgga agatatctgc tgcttggttt gtgccgtctt tttctttatc ttcgtgaaag
      661 actcagtagt agcatccaac tcgactgctc ctgccaacgg tccgtcttcg cgcgcttctt
      721 ccggcggcgc gagttcgctc ctatcgtcgc cggcaatcac cggggcatct ccttccgtgc
      781 cttccggagt aacaataatc gaaaaaggaa gaccttcggg gccgacgttg cggcccagag
      841 cttcgtctgt tttctggcca ccagtgcgtt tggcggcgat tttttgcaat tgctctttga
      901 gtttttcttt gggcagcttt ccgcccttaa gacgattaca cgcttcgaca acttcttcga
      961 tataaagttc gaatatattg cgtcggcgga actcactctt ggcgtgctcg cgtcgtctaa
     1021 gaaatactcc aagccgccga ccacactctt gcagagccag cttgatttcc tttcggatct
     1081 catcgtaatc ggcgatcgct tccttggatt cactggtgaa gggcacccaa actgaagcca
     1141 tatgaatgaa gatcaccatg ggaccagcgg gcagcgcgcc tcgcgattga ggaacgccat
     1201 agtttcgcca ggtggtatcg agcaccgcct tgaatgtgga gcaggcggat tgctgataca
     1261 agagtggcac ccgattggca taacgaatca cgcgcgccag ctcgctatca ttgtcttgct
     1321 gctccccttc agcgagcgca accctaggct gttcgggctt ttcgggcgcc tgttccggtg
     1381 ccttaccata cgccaggccg gcctcgatca agaatgggtt gcccctataa acagagggcg
     1441 gccgggtgac agccgtgtag aactctcctt tgatctgctt gtagagacca tgaagaatcg
     1501 ctctttcacc gatcggggag atacagttgc tgggcggcgc catgatcttg gtcgattgaa
     1561 tcgctttgta aagggcctcg gcagctgatc catgaacatt tcgcgggcgg atgcccggcg
     1621 acagcttggc tgccttgcag atctcctgag cgagattgct cgagacccgg ctgaaatcac
     1681 tgctcaaaaa tccaccgacg gattggcttt ttgtgtcgtg cagcatcctg agtaaaattc
     1741 cgaactcgat gccgtagggg tgcggtttga tttcacgcgg ttgcggcggc agttcctcga
     1801 ttgtccgcgg atactctttg gtttcgccct ccggggtttg ataaacgagt ttcacgtgcg
     1861 gattggcgat ggcggtctgc tccaaatatt catccaccga agcccgccct ttttgatagc
     1921 gcccttctat ttcgagagtg acctgagtgc cgcgatggtt ctcccagtcg atcttcttgt
     1981 tctcaagaat ccgcggctca tttttcttgg tatcgatctg cacctcgaaa tagtgagcag
     2041 gggacttggc gctgatgcga gaagtgatct gcaccggttt gccggtagtg agttgcgcat
     2101 acatgccggc agccgatatg ccaatgccct gctgcccgcg gctcatgcgc aggcgatgaa
     2161 atttcgaccc atagagaagc ttggcaaaga tcttcgggat ctgctggcgg acgatacccg
     2221 gcccgtagtc gatcaccgtg attcgaaaac gatttgcctg gctcggtggc ggcgctgcat
     2281 ccccattcgc ggcgacttcg agtttgacga tgacttcggg caaaattccg gcttcttcgc
     2341 aggcatcgag cgcattatcg acagcttctt tgacgcaggt gagcagcgcc tttcttggat
     2401 tgtcgaaacc caataagtga cggtttttgg taaagaactc ggaaaccgaa atttctctct
     2461 gacgcgcgcc catttctact gccgatagta agatcttcgg cgccgcaggt ggaacttcga
     2521 ttaagtcagt ggactttgct tgggccttgg acattttgat tatttgctcc gtgaatcaga
     2581 atcagtggta gctgattgag tctgagagtt gactacgttg aaagcacttc gctcatcgaa
     2641 cgtagcgtta cgaatttatg gatacgggaa gctatgttca gtgattgagc ttgctagtat
     2701 accagacgcg agattttttt tgtactagcg ggactggaaa caacgacttc gatgagcttg
     2761 ttttggttca gagatcgaag gcaaccggat gaaaaggaag ttttatgcgg caggaataaa
     2821 ttgcgaggca aagctcgccg ggcgtagctt tggtgatgct ttcatttaga taggcacaag
     2881 aatagatcgg ctgtccgcct ctttactgcc agccgtaaaa acgttttgcg ttctctccca
     2941 aaattctttt ctttgcctca ccggagatgt ccttacggcc gcgaatcgtt gatacgacgt
     3001 acggaaacat gccgtcccag tgcggataat cggaagcaaa aaggacctta tcatcgccga
     3061 ttcgttcgag cacataggga agggtctctt cctccggctc ggtggcgaaa aacaggttgc
     3121 cgtgctttac gtactcgcta ggtttttgct tgcaggcagg cgcttccacc ttgccgcgct
     3181 tctcccattc ctcatccatt cgttccatcc aataaggaac ccagccgacg ccacattcaa
     3241 gaaatgcgat ccgcaacttg gaaaagtgtt ccggcacgcc accataaatc agaccggcaa
     3301 actgaatcac cgtttctacc ggccggccga tggtatgcat gaacatgaaa ctgtcgaagc
     3361 gaatttctcc agccggtccc gcgcgccggt tgtgcacgca gagcggcacg ttgagccgct
     3421 gaagctcatc atagatcggc cagaaagttt gcgagccgag gtgttctttc attccttgag
     3481 tcgcgaccgc aatagccgat agtccgagct tagtgaccgc ccgatttgcc tcttcgacag
     3541 ccgctgctgg atgttgaaac ggcagcagcg cgacgccttt taaacgcggc gattgccggc
     3601 acacttcgcc gatgaaatca ttgtatgcgc gggcataagc aaccgcatag ttacgctcga
     3661 tcaaaccgtt gaccgcaaaa ctgctcgtcg gaaacaagac tgaggtgtca atgccttcct
     3721 tgtccatatc tttaagccga gttgcaacat cacaatcctc taatccgccg accaggcctc
     3781 ccagaccggt atccataccg tccgatggca gaagaggacg accgcgcttg ctatgcggct
     3841 cgggtaaatg tttccgcaca tcggcatcct tctcgacgat gtggccatcg gcatcaatcg
     3901 tattcataca acctccttgt tccagccact gaagtttaac aaatgttgat taatctgttc
     3961 cctcgtcatt cagcggcatt aaccactctt cgtgaacatc gacccaaaca gaatccgaat
     4021 cttggcatgg cgaaaccgcg cttagatcga acctgacagt gtacagcggg tcatagagtc
     4081 cgcgatgatc tttgggattc tctaaaatac cgtgcaccag accgatgaca ccgcgcttgc
     4141 cgcgcacata cgtcggcgtt cgtgggttat tgcgatgggc catctcagta gcgacaacga
     4201 ccgcatcacc gatcttgaat ctgccagcac tcgtgttcat agggttaatc aatcgggccg
     4261 cactctaggc gtctccggcg ctgctcccgc aatccgagcc tcattccact gagaaggttc
     4321 catcggcaag ccggctccga tcaagctgtc gacggtgaca agcgtagcca gctcctccgc
     4381 cgacattcct tcggtgccgg cgggtcgctt tggcaaaaca aagtagcgca catccgacgt
     4441 gctatcccac acccgcacct cttgattggg agcaagctcc agtgaaaaca tctccttgag
     4501 cactacgcgt ggggtctgca caattcgttc tttatagacg tcatgcttat accaccaagg
     4561 cgtgtcgccg agaatgtcat agggataaca cgagcataag gtgcagacga cgacattatg
     4621 aaccgcatcg gtgttctcca cgactcgcaa tttgccgatc tttccttgat ggatacccat
     4681 ttctctaagc gcttcccggc cggttgtcag cagggcctgt ttaaattccg gatcggtcca
     4741 tgctttcgcg acgatgacgc tgccggccca agggcgctct tcatgaagtt gctgccagcg
     4801 ctgttctagc tcttgtcgcg ttgcccattt gcgcgcgatc agggcttgaa cgaaaacttc
     4861 gagcaaatcg tggtagtcct tcaagtcctg ctccagattg agcatccgtt cttcgagcgt
     4921 accgtgatgg tccttttttt tgattctgaa aaagtcatcg taatcgccgc gctctttacg
     4981 gttatcttcg atcaccatgt ccatgccttc gacactgcgc gaagaaacaa gcgcaatccc
     5041 cttgacgtac tcgtcgagcc attcttccag gaaacgcacc cgggcctcga cgtttattcc
     5101 ctgagctttc ctcggcttaa agaattggtt gctacgtttg agcgcagccc gcgctttgag
     5161 caaatcgtaa aaagtgacta ctccgcgacg gatcaaatag atgattcgtg gctccaagcg
     5221 tttcgcgcga tagtattcca agtcctcttc aacggccttc acatgagctt cgtagtcgtg
     5281 catgtgccct tcaaaaggct tgtgtccatg ttttcctgac atatcaatct ccaagcttgc
     5341 cgcttgaccc tccccgggcg cttatcgctt gctgaagatc ctcgttttcc cgccgattct
     5401 ttgcctgtga accattaaca atcattcctc cagagatcag tgagtatcga aaatctcttg
     5461 gtactgcttc agcctgtctt tcgtggcatc gacctccgcg gcgctcggct ttgccgcatc
     5521 cactgccggc ggcaagcatc cgaatttcag gagacaggcc atcaacaaca gccgcgcttt
     5581 ggtagcggta agattattgc cggcgatgga caaagggtct cgtttggcgt cgacaaagcc
     5641 gctggcattg cccctgccga ctttaaccac cggcatgccg ctaaaagtgg ctcgtttgag
     5701 ggccgcatca acggaattgc tcatcgcacc gaaaggcgct gagccttcgg cgataaaacc
     5761 agccagcggc gcatcgcgga ggttcttgtc cattcgcgcg agaatatcga cttcttgctc
     5821 ggcgccgccg gcagtgtttt cgggtaggta gcgagcgtgc ttgagtagag tgacctttgg
     5881 aattgcagcc ggcaatagat ttcccgttgc ttccttgatc ggcaccgcaa tgaggacgac
     5941 ttctgatcct tgcattctgg aaccttgaac ttgattcggc agatgcgctg tattcaccag
     6001 agagttgtgc gtatgccgct tgatcgtttt aaacgagaat tgcggcatgc cgggatggcc
     6061 aatccggccg ataatgccgc cgtgacctcc cgtggcaacg taaccaccag gccgcgcgtc
     6121 ggctttttgt acgtcgcgcg aagtgaaaat ctgttcgtcg aggatcgcca cggcgccgat
     6181 gcaatctttt cccgactcgt cagcccagat tcgcgagctg atgtaatcca ccgaatcgac
     6241 gatgttgcga tctccgtcat tgccgacggc gccatgcggt ctttgtgagg aatttccaac
     6301 aatcggcacc gtcgtatcga tcaatagatt gagccaataa atcgtctctt cgacaaatgg
     6361 gctgccttcg agccagatag cgccggaata gtttccgctg ttcagcgcgc cttgaacgat
     6421 gttggtgacc cgcgcgaggg ccgacatcgg cggctcttga cgcaagtacg gcggtcgata
     6481 gggaaaaaaa tccgtgccgc ggatttccgg cgcaatctcg ccgtctccga tatcggtgcg
     6541 ttcgccggca cttcgtcctt tggcgtatcc gccggaaggg agcggacgat aaaaatcgaa
     6601 ctcggctttc ccgctaagta aacaaccaac gccctcgtcc gatacgccca gacgatctat
     6661 ttcttcaaac agcctggagc cgtcggggaa aaatggcacc cggcagagtt ctgccggcgc
     6721 atctttctcg gttccgtcca gctcccaggg ctggccgttg ccttgtcgcg ccatataggg
     6781 caatggatac aggccatcct gcggcgtgag catgatttcg taaaccggtt tatcagccgc
     6841 actcgtgcgc tctcggtgaa atgcgccagc ggcatcgaca taaccatccg gcggagcgta
     6901 gagttccgcc gagtcgcgct ccagaggatg agcgctgaat tgttcgacat aaaccgtgac
     6961 tggcgcagcc aggcgttgcg gcctgagaac gtcgaaacga aggggagtac catcgggctg
     7021 attccgcggc gataaaccat atttcgcgcg cgccttgttg ctcgtcacta gtggctcgct
     7081 gttttggatg gtcgcggtcg gtcccgagaa aaccgcgata cgaggctttt taccagtctt
     7141 atttgggtct gccacgcgag tctccgctgc tggtgaatgt tgaaaaatga aactgtatgg
     7201 tcacttgttc cgaagtgggg ttaaagccga tcgcgagact tcaatcaagc gcgccgtgac
     7261 acggatccgc ccaggaaatt atttgagctc gtacggaatc tgctccggcc cgtagccttg
     7321 ccaatatctt tttgtcgccg gatcatagcg gccttgcaag aaaccgccgg ctttttccgc
     7381 ccgaacgata atcgcaaccg tacgctcttg gccgctctcc tcggcatgaa tctgaaaagg
     7441 cccaaccagg tctacttttc ccggacctac gtttactttc tccttctgcc ggatatcggc
     7501 ataatcggat tttgcgccat catcaacccg ctcataccgt tcaatcgact catgaccgtc
     7561 gagcacccca tagagggtcc agttgtgcgc atgatcgtgg atctgtgtcc gcgtctttgg
     7621 gctcttgatc aagccgttga tgacaaactt atattccgga tcttcgtaaa aaagcagatt
     7681 ttgcgcgcgc tcatcgcctt gccggcaagc aggccaatcc ttcgactgtt cttttatcga
     7741 gcgatcgccg atcaaagcct ccagcagcgg cgtcattttt ttccatcgct cagcaggatc
     7801 gttttccaga gcgaaaagct cgcgtgccga tgtgatgaag cgctcaactg cagattgcga
     7861 actggaagtc ataagtcctc ctgtgcccca gtaaccctca agtaattatt agtcagactg
     7921 gccgttctag gtcaagaaga atggcgcata aatcaagggg aggccgctcg gccgcccttg
     7981 tccggagtat gaatagcgaa cttgcggagc gagtttaagt tccgtgagac caggatgaga
     8041 ggtacttctc ttgctctttt gtgagtgaat cgatctttat tcccatgctc gttaatttca
     8101 atttcgcgac ccaatcttcg acggctttgg ggacttcata tacctgcggc ttaagattat
     8161 ggctattctt gaccgcccat tcggaagcaa gcgcctgggt cgcgaagctc atgtccatca
     8221 ccgacgccgg atgtccctcg gccgccgcca agttaaccag ccgcccctcg cccaggagaa
     8281 aaattctctt gccgccgggg agcgtataag catcgacgga gttacggata tttttctcga
     8341 ctttggtgga gagctttttc agcgcgccaa gatcgatctc gatatcgaag tgaccggaat
     8401 tgcagatcac cgcgccgtct ttcataagct tcaaatgatc tgcggagatg acatgcatgt
     8461 cgccggtcag tgtgatgaat agatcgccga tacgggcggc ttctttcatg gtcatggcgt
     8521 caaagccgtc catggcggcc tcgagagcgc gaaccggatc gacttccgtg acgatgacct
     8581 tggcgcccat gccgcgcgcc cttgccgcca cgcccttgcc gcaccaacca tagccggcaa
     8641 cgacaacgcg cttgccggcg atcaacatgt cggtagctct aattacgcca tcgatggttg
     8701 actgcccggt gccgtagcgg ttatcgaaaa gatgcttggt cgcggcatcg ttaaccgcga
     8761 tgaccgggat ctttagcgcg ccgtcttttt ccaaggcgcg taagcgaatc acaccggttg
     8821 tcgtctcttc catgcttgcc gtcaaaccag aagccaaatg ggagtaatcg gtatgtagta
     8881 aagagactaa atcagcgccg tcatccatcg tgatgatcgg tttgtgatcc agcgcggctc
     8941 ggaggtggct ataataactc ttattgtcct cgcccttgat ggcatacgtg ggaatccgat
     9001 cgtgttgcac caaggaagcg gccacgtcgt cttgtgtcga caacggattc gaggcgcata
     9061 gcagcacatc ggcgccgccg gctttgagca cctgaacgag gtttgcagtc tccgccgtaa
     9121 catgaaggca ggccgaaaaa cgcatgccct gaaatggctt ttctttttga aatctatccc
     9181 gaactcgttg taggacaggc atatcctgtc ctgcccacag gatccgcttc ctgccgttct
     9241 gcgctaaacc gatatctttg atgtcgtatt tcatcgatgc catgctgcca acttcctccc
     9301 gccgcaccgc gcttatgcgc acgccgccgc ttttttaaga tcttcaactc gatcggtttt
     9361 ttcccaagtg aagtgaccat cttccggggt gcggccaaaa tgtccgtagg atgctgtgga
     9421 gcgatagata ggccttttca aatcgagcgt ttgaatgatg cccttgggag tcaaggtaaa
     9481 aacttcttga acgatcttgg caatggtcgt ctctgcaatg gtgccggtgc cgaaggtatc
     9541 gacgagtacg gacacaggtt gagcaactcc gatgcagtag gcgagttgaa cctcgcaacg
     9601 ctgcgccaac tctgctgcga cgatgttttt ggcgacatag cgcgccatgt aggcggcgct
     9661 gcgatcgact ttggacggat ccttcccgga gaatgcgcca ccgccatgcc ggcccattcc
     9721 accataggta tcaacaatga ttttcctgcc tgtcagaccg cagtctccct gcggcccgcc
     9781 aacaacaaaa cgtccggtcg ggttgacgtg aaacacggtg tccttgctca ggtactccga
     9841 cggaatcacc gcctttatga cgccgtcgat gatcgtttct ctaagcttcg agtattcgac
     9901 ttcggggcta tgctggctgg aaatgacgac gcttttaacc ttgaccggct tgccgccgcg
     9961 atattccacg gtcacctggg acttgctgtc gggccgcaag aagtacgctt cgccgccttt
    10021 gcggattttt gccagatact tcaccagact gtgagccatt tgaatcggta gcggcatgaa
    10081 ttcaggagtt tcatcacagg catagccaaa catcatccct tgatcgccgg cgccctgctc
    10141 tttatgcagg ccctcacctt cagtcacacc ttgagagatg tcgggagact gctgctcgac
    10201 ggcggtgaga atagcgcaag tattgccatc gaaccccatg gcagaatcgg tgtagccgat
    10261 ctcacggact acctctctag caatctcggc atagctcact tgggctttgc tggtgatttc
    10321 tccggcgatc acaatcatgc cggtcttagc caggctctca caggctacac ggctatccgg
    10381 atcgcgcgct agatgcgcat ccagcactgc atcggaaatc tgatcacata gtttgtcggg
    10441 atgcccttca gacacagatt ccgatgtgaa tagaaaatct ctcccagcca tggcgaacct
    10501 ccagttcttg gaactgatta atccctgtca tatagccaat aagagggggc tattcaacct
    10561 actcgttctc gttgaatctt ctttcgacca gcttttcgag cgctttgacc gcctgttcag
    10621 cgtctcgacc ggctgcttcc acttcgatgg tgcttccctg ggtggccgcc aacatcagca
    10681 cgcccatgat gctgcggccg tcggttgttt gaccatcctt cgagatcgtg acctgcgctg
    10741 aaaagcggtt cacagtttga accagcaggg ctgcggcccg cgcatgaacc ccgagtttgt
    10801 ttttgatctc cattttttta acgaccttga ccatggatca tttttcgatg tcacgatgct
    10861 tgacatgaat gcggaatttg ccgacagcca atcttttatg caactcttcc accagaacca
    10921 ccgaacgatg ccgtccgccg gtgcagccca aggcgactgt caggctgctc ttaccctcgc
    10981 gctcgtagag cggcaacgta aactccagca aagaatacag ccgatcgaga aaagtccgag
    11041 tctcttcatt cttcaacacg tattcttcaa cctcgcgctt caaaccatca tgggcgcgca
    11101 attcgctcac aaagtaagga ttcggcaaga accgcacgtc caagatcata tccgtatcat
    11161 gtggaatgcc gtacttataa ccgaaggacg tcagaaacac gttcattgtg cgcgaatacg
    11221 gcgcctggca aaaacgttct tccatctccc ttttgagctc atgcacgttc aggtcgctgg
    11281 tgtcgatcac tttatcgacc aattcacgca ttgcctcgag cgccttgcgc tcacgcgaaa
    11341 tcccttcctg tatgggctcc tcgcctgcga gcggatgagg tcgccgggtc tcactgaacc
    11401 gcctcaacaa gacttcatcc gaagcgtcga aaaagagcat ctgaacgtgg tgtcccgcgt
    11461 tgcgcatatc ggtcaaaact tgcggcaacg attgtaagaa ctgcccggct ctcaagtcta
    11521 cccctagagc gatccgagtg attccttcgc tgtaaccgac gcagaggtca atgaattttg
    11581 ggatgagcgg ggcgggtaga ttgtcgatgc agaaaaagcc gttatcttcg agcgcccgga
    11641 tcgcaacact tttaccggat cccgaaagac ctgtgatgat gatgatttct agatcatccg
    11701 tcataacaga tcaaagcttc tggtcttcgg ttgcgatgat gtcgtaaaga tcctggctgt
    11761 tcccggcagc cagaagccgg tgccgcaagt cgggatcttt cagcaaacgc gagattctcg
    11821 ccagcgcctt cagatggtcg gccgctgctt cttgaggagc gatcagagta aagaaaagaa
    11881 aggtcggttc gccgtcaagc gatgcaaaat ctaccccttg taaactgcgt gcaaaaaggc
    11941 cggatatctt ttcaaccgtc ggcagcttgc cgtgcgggat tgccacgcct tcaccgatcg
    12001 ccgtagtact aattctttcc cgatcctgca atacgcgcag gacctgagca ggatcgagac
    12061 ccgccggacg atcggcaagt tgatttgcca tctcctcgag gaccgccgat ttctcttttc
    12121 cgctgagatc agcaatcacc cgatcgaggg ttaaaaaatc agtaatcttc actctggcgg
    12181 ctttcttctg ggctcgagct ttatcttatc cttttgtttt cgcaattgtc gttttaagtt
    12241 gtcggtcacc agatcgatgg ctgcatagag atcttcagtt tgttccttag catggatcat
    12301 cccgccgtgg gcgtgcagct ccacttcggc tgtctggcgt tctttggact caactgaaag
    12361 aactacatga gcgtctaaag attgcgagaa atatttgccc agacgatgca ttttttcttc
    12421 aacgtattgc ttcaatgcat cagtggactc ggtgtgtcga aaggtaaccg acactttcac
    12481 gtcgctcatc gtgctgtgtt tcctttgatt tttacttgcg aaaaaacggc tggcgccgtt
    12541 ttgaggatgg aagaataccc atcgcctcgc ggtacttggc aacggtgcgg cgcgctatgt
    12601 cgatggaaca atccgacagc agcgcagcta tgtgctgatc gctgaacggt ttgcgcggat
    12661 cttcgccgct aatgatgccg cggatctttt ctttaacgct ctcgctcgcg acatcctccc
    12721 cgtttccgcc tctcaggccg ctctggaaga aaaatttaag ctcgaacaat ccctgcggcg
    12781 tgtggacata tttattggcc gtcgcccggc tcacggtcga ttcgtgcata tggatatctt
    12841 cggccacatc acgcagcacc atcggtcgca gttggctcac gccatggtcg aggaagtcca
    12901 gctggaactt gaagatactt tgagtcaccc ggtagagcgt ttgttggcgc tgctggatgc
    12961 tcttgatcag ccatgtggcg gcacgcacct tttcctggag atactgccgc gcctgctctt
    13021 ccgccacgcc ctcttggccg accatgcggc ggtaaagggg gctcacgcgt aaacgaggaa
    13081 ctccatcgtc gttcaaatag atgacatact cgccggcgat cttttcaacg aaaacatcgg
    13141 gtagcacagt acgcacatcc tcttgttcaa aaccgcgcga aggtttcggc tcaagggaag
    13201 taatcaggtg ggccgcatcg gcaatctcgt caatggtggt gccgagatcg cgcgcaagct
    13261 tctcatacct tttcgattcc aagaagctta gataatcaga gacgatccga gctgctaaac
    13321 tgtcgccgag ctccaggttt tgcaattgaa ccaacaaaca ttcgcgcaga ttgcgcgccg
    13381 caacgccaac aggatcgaag aattggatgc gctttaaaac cttttccgct tcctccggat
    13441 cggtttgggt agcggcgcaa acctcttcga gcgttaacgc caaataacca ttctccgaga
    13501 ggttctggat gatgtattgc ccgatcatcg actcgcgttc gctaatcttc gacaggcgga
    13561 gctgccaaag cagatgatct tcgagggagg tttttttggt gagactgttc tcccatgagg
    13621 gaggcgcatc gccgtcgtcc gaaccggcct cggcggtcag ggaaccgtgc atggaattgg
    13681 aatgcgtgtc gagatattcc tgccaatcat aggtgccgat cttatcgacc gccgaaagct
    13741 cccgattgac caccggctcg gtttgcccag tctcgcccgt gttttcgctt cgcggctgat
    13801 cgccactctc gctttcttca cttggcccct cttcgagcgc aggattttcc tgtagctctt
    13861 tcgtgaccat atcttccagt tcgacgcgtg acagctgcaa cagcttgatt gcctgctgca
    13921 attgcggcgt catgactaac tgttgggcga gcttttgaac ttgccgaatt tccaaagcca
    13981 tactagaact taaacccctc ccccaagtaa aattttctcg ccttggggct ctccgaaatg
    14041 acttccggcg tcccttgttc gagaatcgcg ccttcgttca agatgtaggc gcgatcgcaa
    14101 atccccaacg tttccctgac gttgtgatcg gtgatcaaga tgccgatacc gcttgtaatt
    14161 aactcgcgga taattttctg gatctccgcc accgcgatcg gatcaacccc ggtgaaaggc
    14221 tcatcgagca agacgaactg gggcgacagg accagcgccc gagcaatttc gactctgcgc
    14281 cgctcgccgc cggagagcgt gaacgctttg tttttggaca gatgacctac cccaagcata
    14341 ttcagtaatg tttcacagcg ttcttgacgc tcgacgctat tgaggtcgag agtctgtaga
    14401 attgcctgca aattgtcttc aacggtcaaa cggcgaaata ccgaagactc ctgcggcaga
    14461 tagctaatgc cggcgcgcgc cctctgataa atcggcgccg cagtcaagtc tttatcgttc
    14521 agcagcactt tcccctggtc cggatgaact agacccacca tcatgtgaaa ggtggtcgtc
    14581 ttgcctgctc cgttcgggcc cagcaagccc actacttccc cgccttgaac gtctaaattg
    14641 atcccattga cgacgcggcg accattatag ctcttcgcca ccgcctccgc cctcaaaaca
    14701 ctcattttgc cgtgctcgag ccggcctcag acttagcatt atctttcttt tgcaattcct
    14761 ccgggaaaat cgtcacctgc actctgaccg agtccttgcc ttcagctaca gcgcggtctt
    14821 gatcgatata gtagatgatg cgcgaacccg tgacttggct gttgccctgt cgcatcactg
    14881 gatttccggt taatgtaacc gtcttggcct tgtcatcgaa gatagcttta tctccggttg
    14941 ccatccgatt gccctgcacc gcgtttacct tcccctccgc gatgatctgt ttcatttgtt
    15001 tcatctcagg atcaaagacc gcggttaagg tttcgctgcg catagtcatt tcggactgga
    15061 tcgttacgac ccgacccttg taagtgatcg tattcttctt ctgatccact tccatccaat
    15121 ccgaagtgat atagatcggc tccttcttgt tgaactcgaa aacgctctca tcacttttgc
    15181 tcttaccatc ctccttggat gcgccgccag caggaccgac gataaagccg gccagcaaaa
    15241 atactgcaag tgcaaaaccc aagctcggga atttcatggt catcgcaact agcttttctc
    15301 ggacttagct ttttgcttag cgagcttctc cggttccaat ttggtcttta catcacgcac
    15361 caagcgtact ttgctctctc ccaggtcaac ctccatactc gcgccttgca attccaaacc
    15421 ttcaccggtc agcgtggtcc gctgcggcat cacaatttgt tcctttgccg gcagataagt
    15481 cgcctcctgt gagttcaaca aataaccgct gtaagagatt tcaatgccac cctctagttg
    15541 catcttctct agctcttttt cgttgagata gacgtgagcg atgttagccg aggcttcggc
    15601 tacttctcca tttttatcgt agtacaaaaa gcgcggctta tggataactg cttgtctctg
    15661 atctttgtcg taactcgctt cctcgcccaa cagttcccac acagttcggc cattttcaac
    15721 tttggcgcgg cgaaaatctt tgatgttgag agccgagtcc ggtaactcgt ccaacaattt
    15781 gataggattc tgtctaatgt cacgcagctt attagcccaa atctcggcgg taaccttgta
    15841 gccaacggct ccaagcgaag caatgattgc caccaataat aataatcttt ttttacgcat
    15901 gggttgatcg atgaattacg accgtaccat tctttgatac ttctgtaaag taataggcat
    15961 gaaaattgct gggcaactta caaagtccta atttcacgag atttttttga cgatgaaccc
    16021 ccatttattc tgagccttca gcaacagctc cgcgatctcg cgaaccgcgc ctctgccacc
    16081 cggtttttcg gtcacgtagt cgacagaccg gaataactcc tggcaggcgt catttacggt
    16141 tactgccaag ccgacgcgat gcagtatcga caggtccgcg atatcatcgc cgacataggc
    16201 gatctctgtc tccgccaaac ccgattgccg ccggatctct gcataggtcc tgcctttgtt
    16261 ttgggttccc tggtgcacga tttgaaggtc cagttctttg gctcgatacc gcacggcttt
    16321 ggaacttcgt gcgctgatca gaccgacctc gataccggcc cccatgagta acacaatgcc
    16381 ttgtccatct cgcacgtcga agtgcttggt ttccaccccg cgatcgtcga ggatgatgcg
    16441 gccgtcggtc aatacgccat cgacatcgag cagaagcaat tttatagctt tggccttgtt
    16501 tcggaccgct gtagaaatgc gcttcacgga tggggttaaa ggccggcttt gaggagatcg
    16561 tggagatgaa tcgcgcccat gggcgtgcca ttctcatgga gcacaaacag gcaagtaata
    16621 ctgtgttgct ccatcgcggc gaccgcttcg gcggcaagac aatcggcccc tatgcttttg
    16681 gcattccgcg tcatcagctg tttggcgtta tattcaaaaa tatcaccttt ggattccaag
    16741 ccgcgccgca ggtcgccgtc ggtgatgacc cccgtcagtt ttccctctcc atcgacaact
    16801 ccggtgattc caagccgctt cgaggtgatc tcgaaaagag cgtccttgat gggcgtggcc
    16861 tcgctaacca gaggaagttt atcgccacga tgcataagat cttcgacccg caagaaaagc
    16921 cgtctgccta aaataccgcc agggtgacgc atggcgaaat cttcttggtt gaagcctttg
    16981 gtctcgagaa gtaccacggc caaagcatcg cccatggcta gagttgccgt agtgctcgcc
    17041 gtgggcgcca aacccatggg acaggcttct tctctgacgc tgacgtcgag aatgacgtca
    17101 gccgcttttg ccagagttga ctccggattc ccggtcatga cgatcatctt aagagcgtgg
    17161 cgcttgaaat gaggaataat tttgaggatt tcttcggttt caccactgtt ggaaacggca
    17221 agaatcacat cgcccttcat gaccatgccc aaatcgccgt gaagcgcatc gctcgagtgg
    17281 agaaaaaagg ccggcgtacc cgtgctcgcc agcgttgccg caatcttgcg acaaatcaac
    17341 cctgatttac ccataccagt tacaacgacc ttgccgttac agccacggag caacttgatg
    17401 gcactgcaaa aatgctcatc gaggcgatcc accagggata gcagtccctg aatctcgatg
    17461 ttcaacacct ctctagcgcg ccccaaaata gctttttggt tcacgaaatc gaactacctt
    17521 tcaccagctc gtctaactgt ttcacggctc tgattaactt ttcaaactcc tttaaggcta
    17581 aagagttcgg accgtcactc aacgcctgat ccggttcttc atgcacctcc atgaatagcg
    17641 catcgatccc caccgctacc gcggcgcggg ccaacgccgg aatatacttg cgctcgccgc
    17701 cggaggcgtg gccgaggccg cctggcagct gcaagctgtg agtggcatca aaaatcactg
    17761 gatatccgag ctcacgcatc cacaccagcg agcgcatgtc ggaaacaaga ttgttataac
    17821 cgaacgagac tccacgttcc gtcaacaaaa cgcgatcgtt gccggttgaa agtatttttt
    17881 cgacaacatt gccgatgtcc caaggcgcga gaaattgtcc ttttttgatg ttaacgactt
    17941 tcccggattt cgccaccgca atgacaaagt cggtctgcct gcaaagaaac gccggaattt
    18001 gcaacacgtc tgcaatctgc tgaaccgggc ctacctgagc cgactcatga acatcggtta
    18061 agatcggcac gccgactttg cgtttgacct cggcgaggat ctccaatccc tgctccagcc
    18121 ctggtccgcg aaatgaatcg cccgaagagc gattcgcctt gtcgtaagac gacttaaaaa
    18181 tgtaagggac gccggctcga tccgccgctt gtttcagcag cgaggcatgc cgcagcgcgg
    18241 attcttgact ctcgataaca catggccccg cgatcaccgc cagcggttga cccgcgccga
    18301 ttttgaaatt gcctatctga accggcttca tagttcgatt ttaaatggca cagagatggt
    18361 tatgtgcgga ccactttcat gcgcgggagt tcggaaagcg cctgtcgctt ttccagtgcc
    18421 gcctggataa agcctctgaa gagcggatgg cagtccatcg ggtgcgactt aaattccgga
    18481 tgaaactgac aacccaaaaa ccacggttgg ttctttagct caatgatttc aaccaggttg
    18541 ctatccggcg acaagccgct cagaaccatg cctttagcgc cgaactcggc gcgataagtg
    18601 ttgttgaact cgtatcggtg gcggtgccgt tctgagattt tctcgcgtcc gtacaacttt
    18661 gccgccaacg tgccattttg tagcacgcac ggataattgc ccaagcgcat cgtgccgcct
    18721 ttggtgtcga tgcttttctg ctcttccatg agatgaatta ccggatgcgg agtctgcaga
    18781 tcgaattcac tagagttggc gccgtccaga ccgcagacgt ttcgcgcaaa ttcaaccacc
    18841 gccatctgca tgccgagaca gatcccaaag aagggaatct ggttttcccg ggcataacgg
    18901 atcgcggcaa tttttccctc actgccgcga tcaccgaacc cgccggggat caatagtcca
    18961 tcgacatcct tgagaacggc ctggacacct tcctgctcga tcttttccgc ttcgatgcaa
    19021 tccagatcga cccgagcttc attggcgatg ccgccgtgga caagcgcttc cagcaggctc
    19081 ttgtaagact ctttcaggct catgtatttg ccgacgaccg cgatccgcac cgaatccttg
    19141 gaattattta agacgtcagc gacgtgttgc cattttttca gattgggggc gccggtccag
    19201 atgttgagct tctcgacaat gcgctcgtcc agcccttctt catgaaagac cagcggcacc
    19261 tcgtaaatcg actcgacatc ctttgccgtg attacggagt tctcggcaac attacagaag
    19321 cgcgcgattt tggatttgat cttcggatcc aggaaacgat cggtccggca gagcaggata
    19381 tcgggctgga tgccaagacc ggtaagctct ttgacgctgt gctgtgtcgg tttggtcttc
    19441 agttcgccgc cgccggtaat gaaaggaacc agggttagat gaatatagag gacattctca
    19501 cggccccaat cccaggcaaa ttgccgcacc gcttccaaaa atggcaggcc ctcaatgtcg
    19561 ccgacggtgc cgccgatctc gccgatggcg atatcgaatc cgtccgctgc cgctaggatc
    19621 cgccgtttga tctcatcggt gatgtgagga ataacttgaa cggtgccgcc gagataatcg
    19681 ccgcggcgtt ctttggcgat aacggtctcg tagatctgac cggtagtgca gttgttcttg
    19741 cgccccatca aggtcgagac ataacgctcg taatggccaa gatcgaggtc cgcctcggcg
    19801 ccgtcatccg tgacaaaaac ctcgccatgc tgaaatgggc tcatggtgcc ggggtccaca
    19861 ttgatgtacg gatccatctt caacatcgtc actttaagac cgcggctctc caacaacgcg
    19921 ccgatagaag cagaggccag tcccttgccc aacgatgaca ccacgccgcc ggtgacgaaa
    19981 atgaatttgg tttttacagt cgccatgggt tcctttcagt tggtacgatc tctaaacaat
    20041 aataatccgc gctctgaaga atcgatttgc ggcgccgcca ctccgcaaac gattgatgca
    20101 tttagttttt agtgcagccg agtcttcctt tcatctgtta cggcctttta gatagcgctg
    20161 cgctctctgc agatcttccg gagtatccac ttctacggat ctctcatcca cctgcaccac
    20221 acgaatccgg taaccatagg ccaatgcgcg cagttgttcc aaactttcga tctgctccaa
    20281 cgcggtcgga cgcaaacggg caaacttcag tagaaactcg cgctggtata cgtaaagccc
    20341 caagtgccgg taaccccata atcgcctctt tacgctgcgc cgtactttac cgccgttgag
    20401 atcgattgca tcattgcgcg agtaaggaat ggccgcccgg gaaaaataca aagcaaatcc
    20461 cttgcggtcg atgacgacct tgacgatgtt gggattgagc cattcgtcct gatcaagaat
    20521 cgcggtgcaa accgtcccca tgggaatcga tggatcgcgt ttcatcggac ggaccgcgcg
    20581 agtgatcgtt tctgcctgta taaacggcaa atcaccctga acatttacca gcagatcggc
    20641 tctgactttg cgcgccacct cggctagccg atccgttcca ctcgggtgat ctctggcggt
    20701 cattatgact tcgccgccga attttctgac ggcttcttcg atgcgcagat cgtcggtggc
    20761 gaccacaact cgatccaatt ccgttgccct ggaggtactt tcgtaaacgt gttggatcat
    20821 cggcttgccg ccgatccagg ctaacggctt tcccggaaga cgggtggaac catagcgcgc
    20881 cgggataatc gccacaactg acataatgtg cgcttcagag gtgcgatgac tgcgaaagaa
    20941 aggtcacggt tgcgttctcg tcgttgaaat aaaaaaccgc aatgggcttt cagcgaaagc
    21001 gcatcacgga attcaataat acgcccacgc caaaagggtg tcaatgtgat ttgctgcagc
    21061 gagtctttcc ccgcctcatg ttcggcatca aatcgacgcg tgcggttttc gagaagcggg
    21121 gccagccaat ttgacttatc gcggtcatcc tatgatagga gttatgagga agatcgggag
    21181 gaagaggatg ggcaacttga taaaaatcga tgcggggttc gccaccatcg ggcagggtgc
    21241 gggccctatg atggtaacct ggaaggccgg cttatttaac aagcacggcc tcgaggtcga
    21301 caagccgcgc attatgggcg gcgccaaagg agttgtccgc ggcctgatgg caggcgaaat
    21361 ccagttcggc aatttggcgg cgccagcgcc gttgaggtcc aatgtcaaag gcgaagcgga
    21421 tttgattttt ttgaccggcg gcatcaatca acaattcatc atgggcagac cgggcatagc
    21481 cagccgcgag caactcaccg gcaagaaaat gggtttcgta ggcgacggcg gtctcaatga
    21541 cgcgctggta aatttcatta tcgagaagtt gtcggacgcc ggcatcaaag gcatcgacaa
    21601 ggaacctatt cctgccggcg gcgctgagca aatggcagca ctgattggcg gaaagtgcga
    21661 tgccatggtt atcactccgc cggaatccat tgatgcccgg cgaaaaggct gtaactacct
    21721 gatcgatctc gccgaatacg gtcttaatta cgcgctgggc gggattgccg cgcgccgcga
    21781 ttatgtggag aagaatccgg agattacgca caaatttatc aaagcatacg tcgaagggat
    21841 gcaccgctac cggattgacc gcgatttcac tgtcgaggtc caggctgagt atagcggcat
    21901 tgcggaccgc acagtggcgg aagaaactta cgatctcacc cgcccgggta tgccgccgat
    21961 tccttacccg gttgttgctt cgctgcaagt tttactcgat tttatgtcca aggaaattcc
    22021 tgaggcgaag aatgtcgatg cgcggcgctt tgtcgatgat cactttatcc gcgagctcga
    22081 ggagaacgga tttattgctt cacttgcagg tgttaaatga gttcggggca ggctcccatg
    22141 atcggacgct cgatgtcgcg caccgcagat ctttaaggag aaaaccatgc gcgtgatgtt
    22201 tctgaaccac tctttcaagc gccactctgc cgagctcgaa actcacatcg agcgacttct
    22261 gaaaagttac gcctcgcccg agaccacgtt tgagcttgcc tatccggagg atcttggcgg
    22321 cggcgaagtc ctgagcctgc ttgaacaagg aaaagcatta tccggtctac accacattct
    22381 ggaaacgccc gctttggtca aaaaagctat tgaagccgag caactaggat tcgatgcggt
    22441 tattcagagc aatactttcg atcccggtgt tgaagccagc cggcttgcgg tgcgcatacc
    22501 ggtcatcggc ctactgcgcg cttccttgca ttttgccgcg actatttgcg acaagttcgg
    22561 tttgatcgtg ccgctgccgg cgcacatgcc tcataccatg agattggtgc aatcctacgg
    22621 catgaatcgc ttcgtaaccg gcatcaaaac cgtagacctc tacgacaccg gtgacttgtc
    22681 cggttatcac gatctcgtca tcgagcgaac ccttgtcatg ggccgcgagc tcattgaaca
    22741 aggagcacaa gcgattattc cactcggagg caagatatac ccgtacgttg tgacaccaga
    22801 agagctggcg cccaacctcg gagttccgat catcaatacc aaagctgtgg gcgtaagtta
    22861 tgccgagctg atggccagat cccgaatcac acacagccaa cgagcctatc catggtcgcc
    22921 gggcttaaat ccggacaaag tgtcaatgcg ccgttgagag ctactttaaa tcatgctgcc
    22981 agtgacgcgc agcatttttg ccgctggcag agttaacaat tcacaatacg aacttggctc
    23041 gcgtgaaatc accacccatg gcgaagaaca ccgcatcttg gggtatcggt atcgctgacg
    23101 gagacagact atgattattg acgctgatgg gcattttttt gagactgaag agattttcga
    23161 cagatacatg gaaccagacc tgcgaaatta ccggcctcgc cttctgagcg acgaagatgg
    23221 ctacaattac tgggtcgttg acgggcaaac gtcgtaccgg cggccatcgg tgaagggagc
    23281 cggcgctcct ggcaccgcag cacctcccgg aaaaaagatc caggcggcgc gccgcgcttc
    23341 cgccggcagc cagaccttga ccaatataag ggagcggctc gatgatttag ataaagaggg
    23401 tatcgatatc caatatctct acccgagttt tttgctccac atcaatgcat tagccgatgg
    23461 catcctagca atgggcgtgt gccgtgctta taacagctgg ctggctgagg cttgccggaa
    23521 agcggcggat cggctgaaag ggatcggcgt ggtttctcta caggattctc aaggcgcggc
    23581 aaaagaatta tcgcgcatca aagacctcgg catgaccgcg gtcatgatta acggcactgc
    23641 cgggagcaaa cggctggacc acccggacca cgatccattc tttgccgaag cggaccggct
    23701 gaacctgccc atcgccgttc acttcagtct gcaatttcca acggtcgaca agctcttcga
    23761 gcatcatttt cccaaccgag tattggcggg tatttttccg atcatggcgg gacttaccag
    23821 cgtgctgtgt tctggattgc tggaccggtt taaaaacatc aagttcgcgt ttcttgaagg
    23881 cggcatcagc tgggtgcctg cccatgtcga gcgtatggat gatcactttg aaaacccgcg
    23941 ctacggggcg cgagacctaa ttagtcatcc accgagcgaa tacttgaaat cgggacggat
    24001 attcttcggc tgcgaaggga acgaaccctt tttgcctcgg gttatagccg atctaggtga
    24061 agagctattt atgttttctt ccgattatcc ccacgctgac cgcaccgaag gtaccgctgg
    24121 gtatctaaaa acccggagcg atatcggtat gaacgtgcgg cataaattac tggccgataa
    24181 tgctcagcgt ttttacggca aatgagctag atgggcgcgt ttagatttcg cgcttcaata
    24241 attaattgat aattccgaca ccggtagcat cccgcacttc aaaaacctct aagttggatt
    24301 gaagcgaatt ggaaccggcg cgtctaatca gttaagggag gagaacatat gaaaaaaaac
    24361 atagttaaat tggtcgtagt tgccttcact gccttggtct ttggtgggtc gccactgcgc
    24421 aagatacgaa agccgataaa atgggcaagt cgccggcgga ttctccaaag atggagaagt
    24481 cgacaaaggc gaaatcgggc acagcaaccg gcgaggttac ctctgtggac gccaagtcgg
    24541 gccagctcac cgtcaaggca aaggacaagg accttaattt gatggccgag tccaaatccg
    24601 ccaagaacgc gctggataag ataaaggttg gcgataatgt ccgagtaacc tacaccgata
    24661 aagacggtaa gatggttgtc agctcggtga aagcagactc caagggtatg agcaaatcgg
    24721 aagagagaaa gtccagcaca agggctgaaa agaaacagcc ttaattcgtt ttatcgccac
    24781 ttcttgaggg gtagctgaaa gcatcggcta cccctttttt gttccttgca accctttatc
    24841 gatcaactta agaggctatg cctgctggga agcttattgc tttcaaggtt cttgtctact
    24901 agactttttg ttatgaggca gtagctgttt gtgtctgaaa aggaggagtg gagatggatc
    24961 tgccgaaatg ccaaaagtgc aacaacggga ttttaatccc gttatccgat tatgggcagg
    25021 atggtgcttc ggtgatgttt aaagcttggg cctgtaccaa tcccgactgc gggttctctt
    25081 tacgagtcga taagggcgaa gtgacttacg gcaagagaat cgagccgaag cattaaccac
    25141 cgatcccacc acgcaagctg tcaccaagcg gcatctcaag ccggcaagct ttgataaaaa
    25201 ctctgaatcg ccttaccaaa aagttcgcag cctatcttta aagcttccaa acatctcttt
    25261 tgagtttcac catctgtggc ataacgtctg agaatttcag gcgcaatcca ttcatgcccc
    25321 gcttcgtcgg ctagcaaagt atcgcggtaa accgacgccg tctgcgggtc gatggattgg
    25381 acgcgttcaa taaacgattt tgaggttctc ggaaaaaact cctcgtgacc aaattgaaca
    25441 ctggcgatca actcacaaag cgaacccaat gaagcatgat aatcccagag ttcctttagt
    25501 tctttcagcg gctgattcca gagcgcgtct gggtcgccat ttaattcacc gattcttttg
    25561 cgcaagatcg acgtgtgttt taattcattg accacatatt cggacatcgg ctgttgcaga
    25621 tcgaggtcgg gaatgtctcc gacccaacgg gcagtgactt cggcggcctt gcgctccatg
    25681 accacgccga actgcaagcg gcgcaacaca atcgccgggg tgagcggttt ttccgcgccg
    25741 taactcttgc gttcggcatt catcttttcc cagaacgcct cccggtaatt gactaatccg
    25801 gtaagaaact cttccgcttt gagagcttcc atttctcctc caatcatctg agcacgcgcg
    25861 cgctgatttg acacgcagcg ccttttgggt tttaacttac aggaaattcc gctgctgtaa
    25921 aggagtattc tgccagtcaa atcttgctca gcttacaacc gaacttaaac ttgatggaat
    25981 cccaagaagc aaaaaatctc gtcgacaact tgtataagga actctacgaa cactgggatc
    26041 gcaagatcaa tcaaggccct tttatgagcc gactgcgaga cgggaccttg ccattgccct
    26101 gtttgcggcg gtttttcaaa gattggggcc ttttctcaat cgaagtagtg gcattgaacg
    26161 ccgtctccta ttacgtgcat ttgccattct tcgtgcgcaa ctttgatttg ctgccggcat
    26221 tttcagacaa gattgccgag gagctggttg ctcccaaacc cccgggccat acactgattc
    26281 tgcttgaaac cgccaatgcg ttaggcctgt ccaaggaaga actgttcgag caacccgcgt
    26341 cggcccccgg gcgggcgatc agcgattatt cacggcgcgt atttcaggat ggctcgatta
    26401 ttgagttgtg gggcgcccat gtatacgaag aaacgctcgg gcactgggcc cagcaatggg
    26461 gcgaggcctt ggtatcgcat tacggcctga gccaacgaca agcgatctac tttaccgcgc
    26521 atgccgaggc cgatctggta gagcacgaag aacacatggg acatgggccg ctcaatcgga
    26581 tgatcctgca gcggatcctg gaacaaggat tgactgcaac caaactcggc tatgatccca
    26641 aatactgcgg tttcactatg gtcgatttgc acgcgctgat ggaacggcac gctttggaaa
    26701 acccatattc gtcagagaat cgctcctgaa actctcatca tctgtgccac gccctcctcc
    26761 gcgacattca cgtccggccg attttcacta cagaaagctg ggttcgtgat ccgcgccggc
    26821 gcagtagacg ttggggtcct tgtcttttta ttgtcctggt gtcttttttc tcaagccggc
    26881 gcagagccgc ccggcaactg gcaaatccgc gcaccgctgc cgtcatctcg caccgaagtc
    26941 gccgccgccg gaatcgccgg caaaatctac gtgatcggtg gctacggcaa gaatggcaac
    27001 ttggttgaag catacgatcc acaaaccgat cgctgggagc ggcgcgcatc gctgcccaaa
    27061 cggctgcacc atagcggcgc cgcggcggtt aatggaaagc tctatattat cggcggctac
    27121 atctccggca tcggtccgat tgacacagtt tacgagtacg atcccgccgc agatcgctgg
    27181 acggcaaaaa agcctatgcc gatcgcccgg ggagcgctcg cagtcggcgt aatcgccgga
    27241 aaaatttttg cggcgggagg cgtcggcccg gacgggaaaa acactgctgc cgttcaagcg
    27301 tatgatccct ttcaagatcg ctggaccgac cacgctccga tgccaactcc tcgag
//
LOCUS       DASUVS010000479         3760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_211920, whole genome shotgun sequence.
ACCESSION   DASUVS010000479 DASUVS010000000
VERSION     DASUVS010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211920"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1877)
                     /gene="cysN"
                     /locus_tag="VF182_25210"
     CDS             complement(45..1877)
                     /gene="cysN"
                     /locus_tag="VF182_25210"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEX6440442.1"
                     /translation="MDTLRFITCGSVDDGKSTLIGRLLYDTKLILKDQLEALTTDSKK
                     YGTQGRRLDFALLVDGLQAEREQGITIDVAYRFFATGKRRFIVADTPGHEQYTRNMIT
                     GASTAQAAVLLIDARKGVLVQTKRHSRIISLLGIRHVALAINKMDLVRWSQSVCDDIA
                     LSYTGFAKDLGFDTITVIPVSALEGHNIVEADKVAMSWYEGPSLLQWLETIEPEDTNE
                     RNPFLMPVQWVNRPNLDFRGFSGRIASGIVRPGDSVRIIPSGVVSRIKEIVIWGGPQP
                     AATAGQSVTLTLKDEIDASRGDVIATASHPVECADQFEADLLWLSDHPMLSGRPYGAL
                     LHTKLATVTITQIKHLLDVNSGAFLAAKTVGLNEIATVNLSFDRLVPFASYCENKRMG
                     AFILIDKVSNETVGAGMIHFALRRSLNVHWHALEVTKTARAEMKHQVARCLWFTGLSG
                     SGKSTIANLLEKRLHAEGKHTYILDGDNVRHGLNRDLGFTEADRVENIRRVAEVAKLL
                     VDAGLIVIVAFISPYRAERQLARSLFDPGEFIEVFVDTPLEECERRDTKGLYAKARRG
                     ELKNFTGIDSDYEPPETPEVRLRTIDMKPESCVEMIVRSAFGSQ"
     gene            complement(1877..2776)
                     /gene="cysD"
                     /locus_tag="VF182_25215"
     CDS             complement(1877..2776)
                     /gene="cysD"
                     /locus_tag="VF182_25215"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX6440443.1"
                     /translation="MRQSLLDELESESVEIIREVAATAAKPVMLYSVGKDSSVMLRLA
                     QKAFFPGRIPFPLMHIDTGWKFREMIAFRNRIAIEVGFELITYTNPRGAEEGIGPVSH
                     GSAYHTDVMKTEALRQALDLYGFDAAFGGARRDEEKSRAKERIFSFRMPGHRWEPKAQ
                     RPELWNLYNTRINPGETIRVFPLSNWTEADIWTYIRQENIPIVPLYFAAERPVVKRYG
                     QWIMVDDSRLPLEIGEKPEIRRVRFRTLGCYPLTAAVESNATTLDEIVAETLAATSSE
                     RQGRVIDHDGAGSMERKKQEGYF"
     gene            complement(3183..>3760)
                     /locus_tag="VF182_25220"
     CDS             complement(3183..>3760)
                     /locus_tag="VF182_25220"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX6440444.1"
                     /translation="KIGFKANGIFLMDGSKRSVHSNAYFTGIGKAKHIVLFDTLVEQM
                     TIDQGLAVLAHEMGHYKMKHIHRMMVVQCVILCVGLYILSRLVNYEPLFTAFGLQASN
                     HAALVLFSLLSGPFTFYLAPLMNFLSRHHEYEADRFAATTLANGKPMEEALIKLTVKN
                     LSNLTPHPWYSAYHYSHPTPAERIGAIRQLS"
BASE COUNT          885 a         1029 c          961 g          885 t
ORIGIN      
        1 aagcgttcgt gtcggcggat gcttatttaa ccgatcaaca tccattattg actgccgaat
       61 gccgaacgca cgatcatctc cacgcaagac tcaggtttca tgtcaatcgt acggagacgc
      121 acttcggggg tttccggtgg ctcatagtcg ctatcgatac cggtaaagtt cttcaactcg
      181 ccgcgccggg ctttcgcata gaggcctttc gtatcgcggc gttcacattc ctccaaagga
      241 gtatcgacaa acacttcgat gaattctccg ggatcgaaga ggctgcgcgc caactggcgc
      301 tccgcgcggt agggtgagat gaaggcgaca atcacgatca gccccgcatc gaccaacaat
      361 ttagcgactt ccgcgacgcg acgaatgttt tccactcggt cagcttcggt gaagcccaaa
      421 tcgcgattga gtccgtgacg cacattgtcc ccgtccaaga tataagtgtg cttgccttcc
      481 gcatgcagac gcttctccag caaattggca atcgttgact tgcctgaccc ggaaaggcca
      541 gtaaaccaca ggcatcgtgc cacttgatgt ttcatctcgg cccgagcggt ttttgtgacc
      601 tccagcgcat gccagtgaac gttcagggac cgccgcagcg caaaatgaat catccccgcg
      661 ccgactgtct cattgcttac tttgtcgatt aggataaaag cgcccattct tttattttca
      721 caataggacg caaatggcac cagccgatcg aaactcaaat tgacggtggc gatttcattg
      781 agaccgactg ttttggctgc tagaaatgcg ccgctgttaa catccaagag gtgcttgatc
      841 tgagtgatgg taaccgtcgc gagctttgtg tgtaacaagg cgccataggg acgcccggac
      901 agcatggggt gatcgctcag ccaaagaaga tccgcctcaa actggtcggc gcactctacc
      961 gggtgtgaag ccgtcgcaat gacgtctccg cggctcgcat caatctcatc cttaagcgtc
     1021 agagtcaccg actgcccagc cgtcgctgcg ggttgcggtc cgccccagat gacgatttcc
     1081 ttgatccggc ttactacgcc cgagggaatg atccgcacgc tgtctccagg cctgacgatc
     1141 ccacttgcga tgcgccccga aaacccgcgg aagtcgaggt tgggccggtt aacccactgc
     1201 acgggcatca ggaaaggatt acgctcgtta gtatcttccg gctcaattgt ttcgagccac
     1261 tgcaacagac tcggcccttc gtaccaggac atcgcgacct tgtccgcttc aacgatgtta
     1321 tgaccttcga gtgctgagac tggaattacc gtgatcgtat cgaagccaag atccttggca
     1381 aatccggtat aagaaagcgc gatatcgtca cacactgact gggaccacct gacgagatcc
     1441 atcttgttaa tagcaagggc gacgtgacga atgccaagca gcgagatgat gcggctgtgg
     1501 cgtttggtct ggactagcac ccctttgcga gcgtcgatca gcagcacagc ggcttgcgct
     1561 gtggacgcgc ccgtgatcat gtttcgggta tactgctcat ggcctggcgt gtcggccacg
     1621 atgaagcgac gcttgccagt tgcgaaaaag cgataggcaa catcaatggt gatgccctgc
     1681 tcgcgttcgg cctgcaagcc atcaaccagc agtgcaaagt ccaaacgccg gccttgagtg
     1741 ccatatttct tggaatccgt tgtcaacgct tccaactgat ctttaagtat cagcttagtg
     1801 tcgtaaagta agcgaccaat gagcgttgac ttgccgtcgt ctacgctccc gcaggtgatg
     1861 aatcgcaaag tatccatcaa aaataacctt cctgcttttt acgttccatg gagccggcgc
     1921 catcgtgatc aatgacgcgt ccttgtcgct cggaacttgt cgccgccaga gtttcagcga
     1981 cgatctcgtc gagcgtggtt gcattacttt caactgcggc ggtcagagga taacatccga
     2041 gagtccggaa tcgcaccctt cggatctccg gcttttcacc gatctcgagc gggagccggc
     2101 tatcgtcgac catgatccat tggccgtagc gcttaactac aggtcgttcg gcagcaaagt
     2161 acaaaggaac gatcggaatg ttctcttgac gaatgtaggt ccaaatgtcg gcttcagtcc
     2221 aattggaaag tggaaatact cttatggttt cgcccgggtt gattcgcgtg ttgtaaagat
     2281 tccacagttc cggtcgctga gcttttggct cccaacgatg acccggcatt cgaaacgaaa
     2341 aaatgcgctc cttcgctctc gacttttcct cgtctcgtct cgccccaccg aacgcagcat
     2401 caaacccgta caaatcaagc gcttgcctga gcgcttcagt cttcatcacg tccgtgtggt
     2461 acgccgagcc atgggagact ggcccgatac cctcctcagc gcctcgcgga tttgtgtaag
     2521 taatcagctc aaaaccgact tcgatcgcga tgcgatttct aaatgcaatc atctcgcgaa
     2581 acttccatcc ggtatcgata tgcatcaatg gaaaggggat tcggcccgga aaaaaagcct
     2641 tctgcgccag ccgaagcatt accgatgagt ctttaccgac cgagtacagc atcaccggtt
     2701 tagccgctgt agctgctact tcgcggataa tttcgacaga ctcagattca agctcatcta
     2761 acaggctttg tctcatcgtt ggatactagt ggtcttggta tgtgccccga tggagaacgt
     2821 cccccatttg aaaataggcc tctgggggct ctgtagccta ccgaggggtt ggaataggaa
     2881 agcagtctcc actcgttcat ccctggggga aaaacaaatc attcttcagc gtttccacgg
     2941 gtgtacaccc gaaattcaag ctcgctggta aatcgcacta gtgtaaaaaa ggacattggg
     3001 taccggcggg tcgcaattag cgccaacaac agccgtatag gcgctaacgg tacacgaaag
     3061 tatctttccc agtcaaatat tttgggtgtt agattagcgt ctcaaatgct cgggcatcac
     3121 aacgaaccgg gaagctcgct tgaagctatg tctctcatgt gcttcgtgct cacgcaaagc
     3181 tactaactga gttggcgaat cgcgccaatt cgctccgctg gcgtggggtg cgagtagtga
     3241 taggccgaat accatggatg cggggtcagg ttcgacaaat ttttcactgt taatttgatc
     3301 agggcttctt ccatcggctt gccgttggcc aacgtagttg ccgcgaaacg atcggcctca
     3361 tactcgtggt ggcgcgataa gaaattcatc aacggcgcta aataaaacgt gaatggtcct
     3421 gaaagcaggc tgaagagaac taaggctgca tgatttgacg cctgcaagcc aaaagcagta
     3481 aagagtggtt cgtaattcac cagccgactg aggatataca acccgacgca cagaattaca
     3541 cactgcacga ccatcatccg gtgaatatgt ttcattttgt aatgccccat ctcatgcgcc
     3601 aacaccgcta gtccctggtc gatggtcatc tgttcgacaa gcgtatcgaa aagcacaatg
     3661 tgtttggctt tgccgattcc ggtgaagtag gcattggagt gaactgagcg cttcgaaccg
     3721 tccatcaaga aaatgccgtt ggccttaaaa ccgatcttct
//
LOCUS       DASUVS010000480         5017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_433105, whole genome shotgun sequence.
ACCESSION   DASUVS010000480 DASUVS010000000
VERSION     DASUVS010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433105"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VF182_25225"
     CDS             complement(<1..780)
                     /locus_tag="VF182_25225"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6440445.1"
                     /translation="MNRRCNCPISALASIWLGALLLLGTAHAQESLDQLHKKALQEGG
                     SLTFYGTLAQINAEKVLPAFEKRFPGLKVNHIDATADKLIARAITEARGGKTFVDVFQ
                     LNLENLGQANDQGLLVDRVPPEAAAYPANLKGSYYVATDLQFIIAAWNTNLVKKDEEP
                     KQFEDFVDARWKGRLIAEPRDVELLVGLKHKYNSEEKAVALVRQIAANNVEFHKGHSE
                     LAEFLVAGQGAMCITCYAHHFPTRMKRGAPVNFMLTEGMGNV"
     gene            complement(965..1816)
                     /locus_tag="VF182_25230"
     CDS             complement(965..1816)
                     /locus_tag="VF182_25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HEX6440446.1"
                     /translation="MTNKTVALLHPGNMGTTIGVAAATSGARLVWVSEGRSKASQDRA
                     REAGLRDVKTLTNAVNESDVILSVCPPDAALDLARDVAGHRFKGIYVDANAVSRSTAQ
                     RICEIVTSTGANFVDGGIIGSPVKRAGTTRMYLSGEHAPEIAALFTDSMLDAKTIGAE
                     PGAASALKVAYAAWTKCTDALLLAIRTFAAAEGVDQALLQEWSISQPDLQRRSQHAAA
                     VATPKAWRYVGEMKEIAATFEAAGLPAGFHNAAAELWGRLAPFKDQTDPLPTITDVTN
                     SLGVNGK"
     gene            2080..3060
                     /locus_tag="VF182_25235"
     CDS             2080..3060
                     /locus_tag="VF182_25235"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440447.1"
                     /translation="MVFHFRIALLAIVVAASTAPAQEKTRIYLGASSKTLGYSPLWVA
                     TKKGFFERQGLDVQLVLLRGMPMTVQALAAGSLHFGSGGPEPYIEAAERGLDFVITGG
                     VINGTAQFIIAGKNYKSYEDLRGATFGTASLTGGTITALREALKVRGLEYPRDYKLLI
                     VAGGSSANLAALQSGQIAATTVAAPLNYAAEEAGFNIIGRIIDGVPYFQTNVIATRRS
                     WAEKNRPVTVRFMRGVVQALRWLHENKEPAIEFLSREIQLKPTHARKGWEFYTQNGLW
                     PADGAVTLEGMKYNIRIYAEQTAMKGPLPAPAKFVDQSYLSEAVKELTTQ"
     gene            complement(3158..3802)
                     /locus_tag="VF182_25240"
     CDS             complement(3158..3802)
                     /locus_tag="VF182_25240"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX6440448.1"
                     /translation="MITSIIFDLDGLLADTERLHCMAYQDALRAYGAGITDHEYAEHW
                     VRNGRGITEWISERGLTLDPIALRAHKSRCYLDLLISELRPMEGTLDVLERLHNRKKL
                     ALASSSYPDAVEGVLAGLGIAHYFQVIVTGLDVPNVKPAPDIFLAAARRLGAIPSQCV
                     VLEDAEKGVIAANLAGMRCIAIPNHHTRHHDFSKATRVCSSLNDVTLELLDTLE"
     gene            4204..4722
                     /locus_tag="VF182_25245"
     CDS             4204..4722
                     /locus_tag="VF182_25245"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX6440449.1"
                     /translation="MRKKNSALTAAVIFGVGLAGTPAFSQSAPGEKSVPPSIERTPTT
                     PGREQQLPPSGREIPKGTEGMSASDIKQVQQALKAKGHDAGTSGVMDDKTREAIRAFQ
                     KAEGLTMTGTVDEKTAQALGVSSKSSSGSGASGSRGSSGATGSSGSSGSNGSGAGRSS
                     GSSSSTDKSPSR"
BASE COUNT         1146 a         1345 c         1387 g         1139 t
ORIGIN      
        1 gacatttccc atgccttcgg tgagcatgaa atttaccggc gcgccgcgtt tcattctggt
       61 cgggaaatga tgggcatagc aggtgataca cattgcgcct tggccggcca ccaaaaactc
      121 ggccagttcc gagtgaccct tatgaaattc gacgttgtta gcggcgatct gcctgacgag
      181 agccacggcc ttttcttcgc tgttgtattt atgcttcaga ccgacgagaa gctccacgtc
      241 ccgcggctcg gcgatcagcc gtcctttcca tcgcgcatcg acgaagtctt caaactgttt
      301 gggctcttcg tcctttttta ctagattggt attccatgcg gcgatgatga actgtagatc
      361 agtagctacg tagtacgatc cttttaagtt ggccggataa gctgccgctt caggcggcac
      421 tcgatctacg agtaaaccct gatcattggc ctgccctaag ttctccagat tcagctggaa
      481 gacatcgaca aatgtttttc cgccgcgagc ttcggtaatc gcgcgggcaa tcagcttgtc
      541 cgcggtcgcg tcgatatgat tgaccttcaa acccgggaag cgcttttcaa atgcaggaag
      601 aaccttctcg gcattgatct gcgccaacgt cccgtagaaa gtcagcgaac cgccctcctg
      661 aagcgctttc ttatgcagct gatcaaggct ctcttgcgca tgagccgttc caagcagcaa
      721 aagcgcgccg agccaaatgc ttgcaagcgc tgagattgga caattgcatc tacgattcat
      781 gttcgacctc ccgttgatgt gaagtaaccc atacgttgcc attctctcgg ctgcgcttca
      841 gcccgccgtt ttttacaata atgcgaccaa aaactgccgc accctaacct tttgcgttaa
      901 agctcgtcaa cacaacctta acaccatcca aatacgacat ggactagggc aggataacga
      961 gtgttcattt accgttaacg cccaagctgt tggtcacgtc cgtgattgtc ggaagcgggt
     1021 cggtctgatc tttgaagggc gcaagccgac cccagagctc agcggcggcg ttgtgaaaac
     1081 ccgcaggcag accggcggct tcgaaggttg cggcgatttc tttcatctca ccgacgtagc
     1141 gccatgcctt cggggttgct acggcggcgg cgtgttggct acggcgttgg aggtcgggct
     1201 gagagatgga ccactcctgc agtagtgctt gatcaacgcc ttcggccgcc gcaaaagttc
     1261 ggatcgcaag cagcagcgca tcggtgcatt ttgtccaggc ggcgtatgcc actttcaagg
     1321 cggatgcagc tcccggctct gcgccgatgg ttttggcgtc gagcatactg tcggtaaaca
     1381 gggcggcaat ctccggcgcg tgctcgccgg atagatacat tcgcgttgtt cccgcacgtt
     1441 tgaccggcga accgatgatg ccgccatcga cgaaattggc gccggtgctg gtgacaatct
     1501 cacagatccg ctgagctgta gagcgagaaa ccgcattggc gtcaacatag attcccttga
     1561 accgatggcc cgcgacatcg cgggcgagat cgagagcggc atccggcggg caaacagaaa
     1621 gaatgacgtc actttcattg acggcgttgg ttaatgtttt gacatcacgc aggcctgcct
     1681 cgcgcgcccg atcctggctt gctttgctac ggccttccga cacccatacc aggcgagcac
     1741 cgctggtagc tgcggccacg ccgatggttg ttcccatatt gccgggatga agtagcgcaa
     1801 cggttttatt ggtcatgatt attcccccag atcagccggc aaaaattgcc ggccaatcaa
     1861 tcggtcatct agctgcgttc tgatccgctg ccatgataat ctcggataag gctaacctat
     1921 tcgactgcga gcatgcgagt caataatagc gcgacatttg tacccgcgat cgcggcgccc
     1981 cttgctctga tcctgccgtt cgttgtaaac gaggatcggt ccggctgagc ctgtcataag
     2041 gctcgggact ctcttcgata actcccttag ggaggtcgca tggtattcca ttttcgtatt
     2101 gcactgctgg cgattgtggt agcggcttca accgcgccgg ctcaagagaa aaccagaatt
     2161 tatcttggcg catcgagcaa gaccctgggt tacagtcctc tctgggtcgc cacgaagaaa
     2221 ggcttcttcg agcggcaagg tctcgatgtt caactcgttc ttttgcgcgg aatgccgatg
     2281 acagtgcaag ccttggccgc gggttcgttg cacttcggct cgggagggcc ggaaccttac
     2341 atcgaagcgg ccgagcgcgg gctcgatttc gttatcaccg ggggcgtgat caacggcact
     2401 gctcaattca ttatcgctgg caaaaattac aaaagctatg aagatttacg cggagcgact
     2461 ttcggcaccg cgtctttgac gggaggtacc atcaccgcgt tgcgcgaggc gctgaaagtc
     2521 agggggcttg agtatccacg tgattataag ttgctgatcg ttgccggcgg ttcttcggca
     2581 aaccttgcgg cactgcagtc cgggcagatc gccgcgacca cggttgccgc gccgttgaac
     2641 tacgccgcgg aggaggcggg tttcaatatc atcggtcgga ttatcgatgg ggttccctat
     2701 ttccagacca acgtgatcgc aaccagacgg tcatgggcgg aaaagaaccg gccagtgaca
     2761 gtccgcttca tgaggggagt cgttcaagcg ctgcgctggc tgcacgagaa taaagaaccc
     2821 gcgattgaat tcttgtcgcg cgaaattcag ctcaaaccga ctcatgcgcg caaaggctgg
     2881 gagttctata cgcaaaatgg tctctggccg gccgatggcg cggtgactct cgaaggaatg
     2941 aaatacaata tccgcatcta tgccgagcaa acggctatga aagggccgct gcctgctccc
     3001 gccaagtttg tcgatcaaag ctatttgtcc gaagcagtga aggaattaac cacgcaataa
     3061 acctcgagat gcccgcgccg atgacgatga ctctggtcct gcttgtggtc ttgagaaaat
     3121 aaagctgaat ctctcgccac cacgtgtcac aatcaaatca ttctaatgtg tccagtaatt
     3181 ccaaggtgac atcattgaga gaactgcaga cccgagtggc tttagagaag tcgtgatggc
     3241 gagtatgatg gttgggtatg gcgatgcagc gcatgccggc aaggttcgcc gcaatgactc
     3301 ccttctcggc gtcctccaat acgacgcatt gcgacggtat cgcgccaagc cgtcgggccg
     3361 ccgcgagaaa tatatccggc gctggtttga cattcggcac atccaaaccg gtaacgatca
     3421 cctggaagta atgagcgata ccgagaccgg ccaagacacc ctctaccgca tccggatatg
     3481 acgatgaggc gagcgcgagt tttttccggt tatgcaatcg ctctaaaacg tccaacgtcc
     3541 cttccatcgg gcgcagctcc gatatcaata gatcgagata gcaacgagat ttgtgggcgc
     3601 gcaacgcaat cggatccaga gttaatccac gctcacttat ccactcggta attcctctgc
     3661 cgttgcgaac ccaatgctca gcatactcat gatcggtgat gccggcgccg taagcgcgta
     3721 gcgcgtcctg gtatgccata caatgcaaac gctcggtatc ggccaggagg ccatcaagat
     3781 caaaaataat gctcgttatc atctttattt gacgcggtaa ctaaagttta tcaggctcat
     3841 caaatcgttt gaagctcgga ctgataggtg ttcggcatca taacccccac aaaaaagaac
     3901 gcgaatgaat cgcccttcat tcagcggtgc atgaaatatg cgacagggcg atctggtccc
     3961 gtgtctcgat taaggctcac aatcggaatt gctgtgtctc gattgtgaaa tctttccaat
     4021 aatctgtcgc aggtttagca tggcagctca gaacggcggc tattaactgc gtgacaagtc
     4081 agaaggattc accaatacag cggatatcaa cccggtgaat gtgccggccc gtcgctggca
     4141 tagcggttgc gtttctcagg gtgggcgcaa cgttgttaat tggttagtgc aaaggaggta
     4201 actatgagaa agaaaaattc agctttgaca gcagcggtaa tttttggagt tggcctggct
     4261 gggacaccgg cattctcgca gagcgcaccg ggggaaaaaa gcgtaccgcc gagtatcgag
     4321 cgcactccga ccacaccggg tcgagaacag caattgcctc ccagcggacg ggagataccg
     4381 aagggcacag aagggatgtc cgcttcggac atcaagcagg tacagcaggc gctgaaggcg
     4441 aagggtcatg acgcgggaac atcgggtgtg atggatgata agacgcgtga ggctattcgg
     4501 gcttttcaga aggcagaagg cttgaccatg accggaacgg ttgatgaaaa gacggcccag
     4561 gcgttgggcg tgagcagcaa gtcatcgagc gggtcaggtg cgagcgggtc gcgcggttca
     4621 tcgggagcga cgggttcatc agggtcatcc ggatcaaacg gtagtggagc ggggagatcc
     4681 tcaggttctt ccagttctac ggataaatcg cctagccggt aaacaccggt aaacagtaga
     4741 tggtttaaac gttgcgtttg cgcaattgct cgcttgaatg gagaggccgg gtttagcccg
     4801 gcctctgctc atttctgctc attaatgcct tcgtgccgcg tctatcaaat cgcatgcttc
     4861 cacaaaacag cgactaaaaa aaatcctact attccgcacg tctctacaag gatcggtgtc
     4921 actgatcctt gcctgtgatt tcgaattgcc tcggttcatc accagatgct acgatcggat
     4981 cgacatgata attgtgatca ccggggtaac cggctcc
//
LOCUS       DASUVS010000481        14802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_869302, whole genome shotgun sequence.
ACCESSION   DASUVS010000481 DASUVS010000000
VERSION     DASUVS010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14802
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869302"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..506
                     /locus_tag="VF182_25250"
     CDS             198..506
                     /locus_tag="VF182_25250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX6440450.1"
                     /translation="MPRYILLSTLTPEGRQTMHNNPDRLEEVNKEVDAFGCKIIAQYA
                     VLGGFDFVSIVEAPDNETVAHMSVDLGSRGTIGITTLPAIPTKEFIAKLKGPKQMGHK
                     "
     gene            677..3124
                     /locus_tag="VF182_25255"
     CDS             677..3124
                     /locus_tag="VF182_25255"
                     /inference="COORDINATES: protein motif:HMM:NF012676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX6440451.1"
                     /translation="MNKVTRVDSRRDTEAIGWVKELGDTENWFQSRRFRQITRLHTAY
                     EVVALRGSAHDDYTIARQAADKMYDYLQGLFKAKKQEITYGPFSSTGAVRAVMEGIKI
                     LYLGGWSTSARGSDSEDPGADLANYALDRVPKEGAAWVRALMHQDEVQRSNRMRMTSA
                     QRKKITAIEFSPPMIIPDGDTGHGGEHHVRNLVKKFVENKIGAIHIEDQRPGCKVCGH
                     QGQKVLVSTTEIIARLNTARLQFDVLGVPGVIVARTDSNDATAIDSLEDERDHPFVLG
                     ATNAEIAPFKNVSLAVIKKFYDRGFKEINGHLLYRISDKAYRDAQEWLRKQHLASRVD
                     EAIARIDKEISSLKKLRQQTRREGKGRRDARERLAALEVTARRVAEETLDHVLALIRE
                     AWAERAGLKTYGETVAESMEARLRSQGKLPMSIERWRKFAAQASFCEARERAKSMGIE
                     VFWDYDVPRTPEGFYPITGGRDMAIARGLAMAPFCDLLWRETAKPDLDDDRIWAEAMH
                     EVFPDKLLAYNLSPSWNWDAWGFTDGQIRSFAAELGKMGYVFNFITYAGHQTEALMNG
                     RLARALKEEGVLGFVRLIQRSLRLANDPAQFPQTFVGGPWADRFRRAARGPSLTTSSM
                     GGKSTEAQHRKPVEVPTSVLERWLRMWTEHWSKQGLYKGGELSVEVKERWAGSEEMML
                     NVFDEARDKIAEIIYRVDRDRAGRKFLAVKDQNTVKKYRARRLMTLMHFFLLHRYKTD
                     LVHYVTPTDDNRLSVSRMIQNGVFRAARTDDPNIIALGVDTARAQKIFANDDSLKRFI
                     TNEFRASRGQALMRAVG"
     gene            3276..5459
                     /locus_tag="VF182_25260"
     CDS             3276..5459
                     /locus_tag="VF182_25260"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEX6440452.1"
                     /translation="MANSVTIGGLKVDQELYGLVRDEIAPGTGVDPQSFWVSLGQIVA
                     DLGPRNRQLLARRKELQNQIDDWCRTKRGRPLDEKEYRAFLIDIGYLVREGENFQVTT
                     TNVDREIAEVSGPQLVVPLDNARYALNAANARWGSLYDALYGTDVIPEADGAEKGEAY
                     NPRRGAKVIARTRAFLDQAVGLDRGRFADVTELTIKQSNGATLLIAKLKDGRETGLMD
                     PRKFAGFVKKDTELASLLLKNNGLHIEIQIDRDHPIGKSDPAGIKDVFLEAAVTTIQD
                     CEDSVAAVDAADKARVYRNWNGIMKGTLEATFERNGRIIMRSLNPDKIFTAPDGESAT
                     LPGRSLLLVRNVGLHMYTDAVTTADGEEIPEGFLDAMMTGLAALHDLKGNSKYINSRT
                     GSVYIVKPKLHGPEEVAATVELFARVENALGLERDTLKIGIMDEERRTTVNLKEAIRA
                     ARERVVFINTGFLDRTGDEIHTSMELGPMLPKMEIKNQRWLGAYEDQNVDIGIETGLV
                     GKAQIGKGMWTMPEEMRKMVETKMAHPEAGANTAWVPSPTAATLHALHYHQVDVAGKQ
                     AELVRRGRASIEEILTPPLLDRTLTPEEIQKELDNNAQGILGYVVRWVDQGVGCSKVP
                     DINNVGLMEDRATLRISSQHMANWLHHDIVTEQQVRKTFERMALVVDRQNQADPNYRA
                     MATIFDKSIAFQAAMDLVFKGREADNGYTEPVLHPRRRQTKGAER"
     gene            5494..6090
                     /locus_tag="VF182_25265"
     CDS             5494..6090
                     /locus_tag="VF182_25265"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017323504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6440453.1"
                     /translation="MKKNGNLTAKRIRTLLCGIILAASMAGIGWDEQPISATTSLREP
                     DVHYEPTSPDVVQVMFRLADVKAGEVVYDLGCGDGRIVITAVREFGARGVCIDIDPQR
                     IAESRENSRAAGVTDKVRFLNQDLLETDVSDASVVMLFLSPELNLKLRPKLLRELQPG
                     ARIVSHWHDMGDWKPQKAARVKSGGRERSVYLWTIPPR"
     gene            complement(6189..7016)
                     /locus_tag="VF182_25270"
     CDS             complement(6189..7016)
                     /locus_tag="VF182_25270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6440454.1"
                     /translation="MELTTETKLKLNDGHMIPQLGLGVWQISSAKTSDVVRAALEAGY
                     RHIDTASAYGNEESVGAAIRMSRIPRESIFVTTKLWNSDHGNPERALDTSLRKLKMDY
                     VDLYLIHFPVRQRRQSWRALETLQRKGKTRSIGVSNFTIAHLSELLAGSETVPAVNQV
                     ELHPYLYQQELLDFCRAKGIVIEAYSPLTHGERLKDPKLVAIARKYSSSKTKSTAQIL
                     IRWALQHGLVVIPKSSNRRRIFENADVFDFQISDDDMRLLDSFNENLRTCWDPSEAP"
     gene            complement(7218..8852)
                     /locus_tag="VF182_25275"
     CDS             complement(7218..8852)
                     /locus_tag="VF182_25275"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6440455.1"
                     /translation="MADEILVVQGGKLIDGTGRTPIDNSTILIHEGKFTAVGRNGEVE
                     IPAGAQVIDVSGKTVLPGFIDGHGHLEDFHGELYLHLGITTCANIELYQDGPWTRAQK
                     QGTDLGKIRGPRIWMSGRAIGGVGTGHDAFGSRTFRDNIIVTTTDEVRRAVQRKKELG
                     CDILKVNEFLSLDLVKVAVEEAHRLEMAVAAHSWDVVGYCDAGVDAIEHIWSVGYSSI
                     PYAPARRKLAEDRLGGVIDQELAGAYYQTENFDEVIGAMVKHQVAWTPTIAKWLRPLS
                     PSAHRFREREKQLLDDPNADLPPAVRVVTDNSYDKLLKRYTPEQRERARIGYDKANEF
                     IRRFVQAGGILKEGSDPPRGMAALLMHQALVMDVEAGVSPMAAIQAATLNVARTFGRD
                     KDYGSVEPGKVADLSIVDGDPLQDIWLTQNVKMVVMDGKVIDIGFKRYKNPIPSFYSF
                     QSLPLDLEISPLFLIEGSGPTVLKVRGAGGMWPFHQVMLNGEPLPTSFVSKDEVKAMI
                     SPEAIANAGTYIVTLKCEGEPFPESHRAHLVVGFKP"
     gene            9235..9681
                     /locus_tag="VF182_25280"
     CDS             9235..9681
                     /locus_tag="VF182_25280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010872620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6440456.1"
                     /translation="MKLYVRFHALALLLFLPAVVFAIDENPAVKVTQLLKTTSAWDGK
                     QIVYPEGQAEMTALLIEIVPGESTGWHQHPVPSFAFLLEGTLEITLTDGRVKRMQPGE
                     ALSEVTDAMHIGRVLSKDPVKIVVFYAGSVGKPLTVNQSSEQKTPQ"
     gene            10107..11384
                     /locus_tag="VF182_25285"
     CDS             10107..11384
                     /locus_tag="VF182_25285"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6440457.1"
                     /translation="MSELPGSQEHKAMLHSHFGFREMPFGVTPDPRFFYSHPHYLEGL
                     AALVHGIEAKKGFMLVTGEVGTGKTILLRKLMRHLDGRVQFIFVSNSHLTSYGLTELI
                     VQNLAISNKEKSRLEMLQDLNSYLVQQLRADRTVALLIDEAQKLSDEALEGLCDLSNL
                     ETDEEKLLQIVLVGQPEIAIKLNKPSLRRIKQRITLHHRLYSLQTPTEVDHYIRHRLE
                     MVGYSGPEIFTREAVEAIWQYSGGTPRLINILCDNTLATACLAAKKKVSAYMIMKSAG
                     GLLLEPGIETPKASADTGLLRMKTAAPRISQRRAETNGGEIGVNFRAVTPLIPLRESD
                     RVKLSRIATKGPAVSMQFFDRMTRAATEAMGPMANRIVVDQISALGESRQTFPQGKLA
                     ELIMRVSVEILNESMRDSFQRTMVREIASLKSM"
     gene            11500..11796
                     /locus_tag="VF182_25290"
     CDS             11500..11796
                     /locus_tag="VF182_25290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440458.1"
                     /translation="MSENSKKLEEPFDHLLRALQEGEAWRISQTIQDIDHRLLDCRKS
                     LEEYSRLRSTLRTINNKLSRLGAEPLKVADELATQDLSEIIKSRIDHFKSSGRI"
     gene            complement(11862..12890)
                     /locus_tag="VF182_25295"
     CDS             complement(11862..12890)
                     /locus_tag="VF182_25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HEX6440459.1"
                     /translation="MTYFRKSFLVTLGGLALGAYLGWRTNHSWTGALATMFIVAVLAT
                     LEVSLSFDNAVVNAAVLTKMSPLWRRRFITWGIAIAVFGMRMVFPLVIVAIIARIGPV
                     SALVLAATDPDNYSRVLTSANVSVSAFGGAFLGMVGLRHFSNREKSVHWISVIERPLT
                     RLGRIEAVELGLVLLLMYFVSTQLAGSEQFQFLAAGIFGLVTYIAVDGISALLKVEST
                     LTGEVVQSGAASFLYLEVLDASFSFDGVIGAFALSNNLFIIAIGLGVGAMFVRSLTIM
                     LVERGTLTSYRYLEHGAFYAIIALAVMMFINAVQHISDAVTGLVGAGFIIAAFLDSIR
                     FNRRVQRA"
     gene            complement(13109..13873)
                     /locus_tag="VF182_25300"
     CDS             complement(13109..13873)
                     /locus_tag="VF182_25300"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX6440460.1"
                     /translation="MTSNHSIHALMVGMSENWWRAGIMPCVRSDLERDKPVAYIFWLV
                     DGPQGPVAVDTGYHPDYVAPEWAQGKNFIEPPALLAQLGVKAEDIKTVIVTHFHQDHF
                     TGLDYFPNAKFVIQRAELEFWTGPLMRHDIFAKQIRPRVRPALERLKQQGRIELIEGD
                     VELYPGLELLKAGGHTPGSQMVALETARGKAVLCGDIAYTLKNIRDHIPVGWYYNLAD
                     TVVALERALTTAARPDLAFPNHDAEIMQGKRLARII"
     gene            complement(14071..14532)
                     /locus_tag="VF182_25305"
     CDS             complement(14071..14532)
                     /locus_tag="VF182_25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX6440461.1"
                     /translation="MVLALALGVTDLATAESMDKDIVEVAASAGSFKTLIAAAQAAGL
                     VEALKGPGPLTVFAPTDEAFAKLPAGTVENLLKPENKDKLTAVLTYHVVPGRVTAGEV
                     VNLSSAKTINGRALQISSMDGKVMIDNARVTATDIMASNGVIHVIDSVVLP"
BASE COUNT         3590 a         4018 c         4051 g         3143 t
ORIGIN      
        1 tttatctcct actctgaatg ggatagccag gaaagcatcg acaagtatcg aaacagcgaa
       61 gcacacgcgg agattcaaag acactcacgg ggactgcaag gcggtcaggc tatcgtgaag
      121 cgatatgccg tgaagtaatc cccaccactg tggaactccg gcacgcaagg aagttgataa
      181 tcgcgaagga gaactttatg ccaagatata ttcttcttag cacgctgacc cccgaagggc
      241 ggcaaacaat gcacaataat cccgatcggc tggaagaggt caacaaggaa gtggatgcat
      301 tcggttgtaa gatcatagcc caatatgcgg tgctgggtgg ttttgatttc gtcagcatcg
      361 tcgaagcgcc cgacaacgag acggtggctc atatgtcggt ggatctgggt tcaagaggga
      421 cgattggcat tactacgctg ccggcgattc ctaccaagga atttatcgca aaactgaagg
      481 ggcctaagca gatgggacat aagtgaaagc tgaccgaatg cgaacctgaa caaatacgat
      541 actttcggca gctttcgagc atttgccgaa tggagaatta acacgccgga gtttcgtcac
      601 actggcggtt ccagatttgc cgcagacggc ataaccggtt cgccgttact gaatagaaca
      661 ttcaggaggt cggacaatga ataaagttac ccgggttgat agtcgacgtg ataccgaagc
      721 gatcggatgg gtaaaggaac tgggtgacac cgagaattgg tttcaaagcc gacggttccg
      781 acaaatcact cggcttcata cggcctacga ggtagtcgcg ctgcgcggca gtgcgcacga
      841 tgactacacg atcgccaggc aggcggcgga taaaatgtac gattacctgc aagggttgtt
      901 caaagcgaaa aagcaggaaa ttacctacgg gcctttctca tcgactgggg cggtgcgcgc
      961 ggtcatggag ggcattaaga ttctctatct cggcggatgg tccacctcgg cccgcggttc
     1021 cgactcggaa gatcctggcg ccgacttggc gaactatgcg ctcgatcggg ttcccaagga
     1081 gggagccgca tgggtgcgcg ccttaatgca ccaggatgaa gtgcagaggt ccaatcgcat
     1141 gagaatgacc tcagcgcagc gtaaaaagat cactgctatc gagttcagtc caccgatgat
     1201 cattcccgac ggcgacaccg gacatggtgg cgagcatcat gttcgcaact tggtcaagaa
     1261 gtttgtcgag aacaagatcg gcgccattca catcgaagat caaagaccgg gttgcaaggt
     1321 ttgcggccat caggggcaga aggtattggt ctccacgact gaaattattg cgcggctcaa
     1381 tacagcaagg ctgcagttcg acgtcttggg cgttcccggc gtgatcgttg cccggacgga
     1441 ttctaatgac gcaaccgcga tagacagctt ggaagacgaa cgcgatcatc cttttgtatt
     1501 gggcgcgaca aacgcagaga tcgctccttt taagaacgtc agcctggcgg tcatcaagaa
     1561 gttctacgat cgcggtttca aagagatcaa cggacattta ctctacagga tctccgacaa
     1621 agcttatcgg gatgcgcagg aatggctaag aaaacaacat ctcgcttctc gagtcgatga
     1681 agcgatagcc cgcatcgata aagagatctc gtcgctaaaa aaacttcgcc aacaaacccg
     1741 tcgcgagggc aagggccgga gagatgcgcg tgagcggctc gcggcgcttg aagtgacagc
     1801 cagaagggtc gctgaagaaa ctctcgatca tgtattggcc ctgatccgcg aggcgtgggc
     1861 ggagagagca ggcttaaaga cttatgggga gaccgtcgct gagtccatgg aagcgcggct
     1921 tcgcagccag ggcaagcttc ccatgtccat cgaacgctgg aggaaatttg ccgcccaagc
     1981 gtccttctgc gaagcccgtg agcgggccaa gtccatgggt atcgaagtct tctgggatta
     2041 cgacgtgccg aggacaccgg aaggctttta tccaatcacg ggcgggcgtg acatggcgat
     2101 tgcccgagga ttggctatgg cgccgttctg cgatttgctt tggcgcgaaa cggccaagcc
     2161 ggatctggac gatgacagga tttgggccga agccatgcac gaggtcttcc ctgacaagct
     2221 gttagcctac aacttgtcgc cttcgtggaa ttgggacgcc tggggtttta ccgacggcca
     2281 gatccgaagc tttgccgccg agctgggcaa aatgggctac gtttttaatt tcataaccta
     2341 tgcgggccat caaaccgaag ccctgatgaa tgggcgactg gcccgggcgc tgaaagaaga
     2401 gggggtgctc ggtttcgtcc ggctcatcca gcgctcgctt cgtctcgcca atgatccagc
     2461 gcaattcccg cagacgttcg tgggcggccc ctgggcggat cgattccggc gcgcggcacg
     2521 cgggccgtcg ctgaccacat catcgatggg tggcaagtcg accgaagctc agcacagaaa
     2581 acccgtcgag gtgcccacca gcgtcctgga acgatggcta cgcatgtgga ccgaacattg
     2641 gagcaagcaa ggtctgtata aaggcggcga actcagcgtc gaagttaaag aacgctgggc
     2701 tggctccgag gaaatgatgc tcaacgtttt cgatgaggct cgcgacaaga ttgctgagat
     2761 catttatcgc gtcgatagag acagagccgg tcgaaaattt cttgcggtga aggatcagaa
     2821 taccgtgaag aagtatcgcg cccgccgact gatgacgtta atgcattttt ttctgcttca
     2881 tcgttacaaa acagatctgg tgcattacgt gaccccgacc gacgataacc ggttgtcggt
     2941 aagccgaatg atccagaacg gcgttttccg tgcagcgcgg accgatgatc cgaacattat
     3001 cgctcttgga gtagataccg cgcgggcaca aaagatattc gccaacgatg attcgttgaa
     3061 gcgctttata accaacgagt ttagggcctc cagaggccag gcgcttatga gagccgttgg
     3121 ttaaaccggg gatgctcagg aaggagacgt aagctgcgcc gatctcgaaa atcttggaat
     3181 tcacttcgac ggccaggctg atttcgaaca ctgaaccgct acgatcgaga tccgcccggt
     3241 gtttatgact cagagacatt tgtgagggaa aagccatggc aaactctgtg accatcggcg
     3301 gccttaaagt tgaccaagaa ttgtatggac tggtgcgaga tgagatcgcg ccaggcacgg
     3361 gcgtcgatcc tcaatcgttc tgggtgagct tgggccaaat cgttgcggat ttgggtccga
     3421 ggaatcggca gcttctggcc cggcgtaaag agctgcagaa tcagatcgac gattggtgcc
     3481 gtacaaaacg cggccggcca ttggatgaaa aggaataccg agcgtttctg atcgacatcg
     3541 gctacctcgt tcgagaaggc gagaacttcc aggtgacgac gacaaacgtc gatcgtgaga
     3601 tcgcggaagt ttcgggtcct cagctggtcg tccccctcga taacgcccgc tatgcgctca
     3661 acgccgcaaa tgctcgctgg ggcagtttgt atgacgctct ctacggcacg gatgtaattc
     3721 cggaagcgga tggcgcggaa aaaggcgaag cctacaatcc gcggcgtggc gccaaggtga
     3781 tcgctcgaac tcgggccttc ttggatcagg ctgtcggcct ggatcgagga aggttcgccg
     3841 atgtcacgga gctcacgatc aaacagtcca acggcgcgac cctgctgatc gcgaagttga
     3901 aagacggccg cgagacggga ctgatggacc cgcgcaaatt tgccggcttt gtaaagaaag
     3961 ataccgagtt ggcgagcctc ctccttaaga ataacgggtt acacattgaa attcagatcg
     4021 atcgtgatca tcccatcggt aaatccgatc ccgcgggtat caaggacgtg tttctcgagg
     4081 ctgcggttac cactatacaa gactgcgaag actccgttgc cgctgtcgat gcggcggata
     4141 aggcaagagt ttaccgcaac tggaatggca tcatgaaggg aacgctcgaa gcgacattcg
     4201 aaagaaacgg ccgtatcatc atgcgctcgc ttaatcccga caagattttt actgcgccag
     4261 acggagaaag cgcgacattg ccgggaagaa gtctattgct cgtgcgcaat gtcggacttc
     4321 atatgtacac cgatgccgta accaccgccg atggcgagga gattcccgag ggattcctcg
     4381 atgccatgat gactggtctg gctgcactgc acgatctcaa aggcaactcg aagtacatta
     4441 acagccggac cggcagcgtg tatatcgtca agcccaaatt gcacggccct gaagaggtcg
     4501 ccgcgaccgt cgagctgttc gcaagggtgg agaatgctct gggcctcgag cgcgacacgc
     4561 tcaagatcgg cattatggac gaggaacgac gaacgacggt caatcttaaa gaggcgatcc
     4621 gggcagcgag ggaacgggtg gtattcatca ataccggctt tctcgatcgc accggcgatg
     4681 aaattcacac cagcatggaa ctcggtccta tgttgccgaa aatggaaatt aaaaatcagc
     4741 gatggctggg cgcctatgaa gaccagaacg tcgatatcgg catcgaaacc gggctcgtcg
     4801 gtaaggcaca gatcggcaag gggatgtgga ccatgcccga agagatgcgc aaaatggtcg
     4861 aaaccaaaat ggcccaccct gaagctgggg caaacaccgc atgggtacct tcgcccacgg
     4921 cggcaacgct gcacgcgctg cattaccatc aagtcgatgt cgccgggaaa caggccgagc
     4981 tagttcggcg cggccgcgcg agcatcgagg agatactcac tccgccgctg ctcgatcgaa
     5041 cgttgacacc ggaggagatt caaaaggaac tcgacaataa cgcgcaaggt attctcggct
     5101 atgtcgtccg ctgggtggat caaggagtcg gctgctcgaa agtgcctgac atcaataacg
     5161 tcggcctgat ggaagaccgc gccaccttgc gtatatcgag tcaacatatg gctaactggc
     5221 tgcaccacga tatcgttacc gaacaacagg taagaaagac ctttgaacgg atggcgctgg
     5281 tggtggatcg gcaaaaccag gccgacccaa actaccgagc gatggccacc atattcgaca
     5341 aaagcattgc gttccaggcg gccatggatc ttgtcttcaa agggagagag gccgacaacg
     5401 gctacaccga gccggtgctt catccgcgac gtcgccagac aaagggcgcg gagcgttaaa
     5461 ctgcttcgtc gtaactctag taaagacgag cgcatgaaaa agaatgggaa tttaactgca
     5521 aagcgaattc gaacactcct atgtgggatc atcctggcgg cttccatggc cgggattggc
     5581 tgggacgagc agcccatctc ggcaacaact tctctacgcg aaccggatgt acactatgaa
     5641 cccacatcac cggacgtggt ccaggtgatg tttcgcttgg ccgacgtcaa agccggtgag
     5701 gtggtctacg atcttggatg cggagacggt cgcatcgtca tcaccgcagt gcgagagttc
     5761 ggcgcgcgtg gcgtctgcat cgatatcgat ccccaacgca tcgccgaaag ccgggaaaac
     5821 tcccgcgcgg cgggcgttac tgataaggtc cgtttcctca atcaggatct tttagagaca
     5881 gacgtgagcg acgcatcggt ggtcatgctc ttcctctcgc ccgagctcaa tttaaaactg
     5941 cggccaaaac tgctgcgcga gctccagcca ggcgctcgca tcgtctcgca ttggcatgac
     6001 atgggcgatt ggaagccaca aaaagccgcg cgcgtcaaaa gcggcggtcg cgagcgctcc
     6061 gtctatctct ggaccatccc gccacgctga caagcctcgc acatgcaaca gtgcgggccg
     6121 gccagccttc ttagaacgcc cgttagaatt tcttcggtgg gatacaggtg agctcgcgaa
     6181 tctaccgctc acggcgcttc gcttggatcc cagcacgttc gcaggttttc attgaaacta
     6241 tccagcaggc gcatgtcatc atcggatatc tggaaatcga aaacatcggc attttcgaaa
     6301 atacggcggc ggttggacga tttaggaatc accactaaac cgtgctgcag cgcccaacgg
     6361 attaggattt gcgcggtcga tttggttttc gatgaagaat attttctggc aattgccaca
     6421 agcttgggat ccttcagccg ctctccatga gtcagcgggc tataagcttc gatgacaatc
     6481 cctttggccc ggcagaaatc cagcaactct tgctgataca aataggggtg aagctccact
     6541 tggttgactg cgggcacggt ttcgctcccg gcgagcagct cgcttaggtg cgcaatcgtg
     6601 aaattgctga cgccgatcga acgtgtcttg ccctttctct gcagcgtttc cagagcgcgc
     6661 cagctttgcc ggcgttgtcg cacgggaaaa tgaatcaagt acaaatcgac ataatccatt
     6721 ttgagcttcc gcaggctggt gtcgagcgcc cgctccggat taccatgatc gctgttccac
     6781 aatttggtcg tgacaaaaat gctctcccgg ggaatgcggc tcattcgaat cgcggcgccg
     6841 acgctctctt cgttgccgta ggctgacgca gtgtcgatat gccggtaccc tgcttccagc
     6901 gcggcccgca ccacgtcgga cgtcttagcc gagctaatct gccacacgcc caatccgagc
     6961 tgagggatca tatgaccatc gttaagcttt aacttcgtct cggtcgtaag ttccataact
     7021 gggcgctcta agttaaagtc ttgcacctcg agctcggtcg tttcaagaaa ggagctgatt
     7081 taacgttccg cttagacacg acacagaaag ctgctttcaa gctcacactc gaagctcttc
     7141 agtctcattg gcgtgccgtg aaatgacgga taattcattg ttcgatcaca atacgaattc
     7201 ctcctgagac aacagcgcta cggtttgaag ccgaccacga gatgggctcg atgggattcg
     7261 ggaaagggct caccctcaca cttgagagtg acgatgtacg tcccggcgtt tgcgatcgcc
     7321 tccggtgaga tcattgcctt gacctcgtct ttggacacga agctcgttgg caacggttcg
     7381 ccgttgagca tgacctggtg gaatggccac attccgccgg cacctcggac tttcaatacc
     7441 gtcggaccgg acccctcgat gagaaatagc ggtgagatct cgagatccag cggcaagctc
     7501 tgaaatgagt agaatgacgg tatcggattc ttgtatctct taaatccgat atcgatcact
     7561 ttcccgtcca tgacgaccat tttgacgttc tgggtgagcc agatatcttg gagcggatca
     7621 ccgtcgacga tcgagagatc ggcgaccttg ccgggctcga cgctgccata gtccttgtcc
     7681 cttccgaaag ttctagcgac gttgagagtg gcagcttgga tcgccgccat cggcgacaca
     7741 cccgcctcga catccatcac cagcgcctga tgcatcaaca acgccgccat cccgcgcggc
     7801 gggtcggaac cctctttgag gataccgccg gcttggacga aacggcggat gaactcattg
     7861 gctttgtcat agccgatcct ggcgcgctcg cgctgctcgg gcgtgtaacg cttgagcaac
     7921 ttgtcataag aattatcggt cacgacgcga accgcagggg gaagatcggc gttggggtcg
     7981 tccaagagtt gtttttctct ttccctgaaa cgatgagcgc tcggcgacag cggtcgcagc
     8041 catttggcga tggtcggcgt ccatgcgacc tgatgcttaa ccattgcgcc gatcacttcg
     8101 tcgaaattct cggtttgata ataagcgccg gcaagctcct gatcgatcac gccgccgagg
     8161 cgatcctcgg ccagcttgcg cctggccggc gcatagggga tcgagctgta acccacggac
     8221 cagatgtgct cgatggcgtc aacgcctgca tcgcagtagc cgaccacgtc ccaactatgg
     8281 gcggcaactg ccatctccaa ccgatgagcc tcctcgaccg cgactttcac cagatccaga
     8341 gaaagaaact cattgacctt gaggatgtcg cacccgagtt cttttttgcg ctgcaccgcg
     8401 cggcgtactt catcggtggt ggtgacgatg atgttgtcgc gaaacgttct cgagccgaag
     8461 gcatcgtgcc cggtgccaac cccgccgatt gcccggcctg acatccagat ccgcggcccg
     8521 cgaattttac ctaagtcggt gccctgcttt tgagcccggg tccatggccc atcctggtaa
     8581 agctcgatat tggcgcaggt agtgatgccg agatggagat aaagttctcc atgaaaatcc
     8641 tccaaatggc cgtgaccatc gatgaatccc ggcagaacgg ttttaccgct gacgtcgatc
     8701 acctgggctc ccgccgggat ttcgacttcg ccgtttcggc ccacggcggt gaatttgcct
     8761 tcgtgaataa ggatcgtcga attatcgata ggcgtccggc cggtgccatc gatcagtttc
     8821 ccgccctgta caacgagtat ttcgtctgcc atctctcttc ctccaaatct ttacaaatcg
     8881 acctttgccg agccatgccc agctcggcaa caagttccat cacgcgatga attctctaag
     8941 gacacctatt gatgaaccgc ttcagtgtca atgcaaatcc ttaacagtcc cgaagattcg
     9001 aacgagataa atcgccgttt gaaatttaat cgcgatcgaa aactcaaaac cagcgaacga
     9061 cgtcgaagca gcaaccctcg aaatcgtagt aacccaaagc tgcggttacg acattgaatt
     9121 ccgcggccta cgaccgcttg cgcgctactg caatccgcat tacaaaaacg cacaccgcga
     9181 tagccaaatc gcggcgccgg gattcacgcc gggagtggag ggaggttagg tacgatgaaa
     9241 ctctatgtcc gatttcacgc gctagcgctg ctgttgtttc tgccagcggt cgtattcgca
     9301 atcgacgaaa accccgcagt gaaagtcacc cagcttctca aaaccaccag cgcttgggac
     9361 ggcaaacaaa ttgtttatcc ggaaggacag gcggagatga cagcgttgct catcgaaatt
     9421 gttccgggag aaagcaccgg ttggcatcag cacccggtgc cttcattcgc cttcctgctc
     9481 gaagggacgc tggagattac gctcacggat ggccgggtta aacgcatgca gccgggggaa
     9541 gcgttgtctg aagtgaccga cgcaatgcac atcggccgcg tgctgagcaa ggatccggtt
     9601 aagatcgtgg ttttttatgc cggttcggtg ggcaagccgt tgacggttaa tcagtcgagc
     9661 gaacaaaaga cgcctcaatg atcgccgtga gcggcacttt tgattcagct cccgagcttc
     9721 gagatcagcc cgccggatgt aactgctgcg cgcgaaatga tctgcggcgg ccatcctgga
     9781 aaaatattcc aaaaaaatct tccatcgatc agaagaatta tccacagctg tggataattt
     9841 tagttttcca gccacgatct atccttaaga agaacatcca cagaatatga acagatgcag
     9901 ttcggtcagc agcgaagatt ttcgtttctg cggcgcgaat ttctgcttga caacttcggc
     9961 tctaaaaatt tacagtcgcg cctcatcgcg ccatcattgg ccacagagtt cttacaattg
    10021 ccaaattttc tcttcgtgtt ttcgttatga cgagcgcaaa tgaaaagttg ttgaacacag
    10081 accgcgttga taaggagagt acttagatga gcgaacttcc ggggtcccaa gaacacaaag
    10141 ccatgcttca cagccatttt ggttttcgcg aaatgccgtt cggagtaacg cccgatccgc
    10201 gattttttta tagccacccg cactacctcg aaggcttggc cgcactggtt catggaatcg
    10261 aagctaaaaa aggtttcatg ctcgtcaccg gagaagtggg aacggggaaa acgattctgt
    10321 tgcgcaagct catgcgccat ctcgacggcc gcgtccaatt catctttgtc tccaacagcc
    10381 atctgacgtc gtatggcctg actgagttga tcgtgcagaa tttggcaatc agcaacaaag
    10441 aaaaaagccg cttggagatg cttcaggatc taaatagcta tctggttcag cagctcagag
    10501 ccgatcgcac ggtggctttg ctgattgacg aagcgcaaaa gcttagcgat gaagcgctcg
    10561 aaggtctatg cgatctttcg aatctggaaa ccgacgagga aaaattgctg cagatcgtgc
    10621 tggtcggaca accggagatt gcgatcaaat tgaacaaacc atctctgcgg cgcatcaaac
    10681 agagaatcac cttgcaccac cgtctctact ctttgcagac gcccactgag gtggatcatt
    10741 acattcgcca tcggctagag atggtcggct attccggccc cgagattttc accagggaag
    10801 cggtcgaggc gatctggcag tattccggcg gaacgccccg gttgatcaat attctttgcg
    10861 ataacacact cgcaaccgct tgtctggcgg ctaaaaagaa agtttctgcc tacatgatca
    10921 tgaaatccgc cggcgggttg ctcttggaac ctggaataga aacgccgaaa gcttccgccg
    10981 acaccggatt actcaggatg aaaacggcgg cgccgcgaat cagccaacgg cgcgctgaaa
    11041 ccaacggcgg tgagatcggc gtcaattttc gagcggtaac gcctctaatc cctctccgtg
    11101 agtccgatcg cgtcaaactt tcccggattg ccaccaaagg ccctgccgtc tcgatgcagt
    11161 tttttgaccg tatgacccgt gccgccacgg aagccatggg acctatggcc aatcgcatcg
    11221 tcgtcgatca gatctcggct ctcggcgaat cccgccagac attcccccaa ggaaagctgg
    11281 ccgagcttat aatgcgcgtt agcgttgaaa ttttaaacga gagcatgcgg gacagttttc
    11341 aaagaaccat ggtgcgggaa attgccagtc tcaaaagcat gtgaaaacca ttgggtagga
    11401 gggatggttc ggcaatagag aggaaccgca gtgtcatcac gtaccccgat aggcgcaatt
    11461 acaagttaga ccaagtctct accggaagga aagattgaaa tgtcagagaa ctctaaaaag
    11521 ttagaagaac cgttcgatca tctgctgcgc gcgctacagg aaggggaagc ctggagaatc
    11581 agtcaaacca ttcaagatat cgatcacagg cttctcgatt gtcgaaagtc gttggaagag
    11641 tatagccgtc tccgctcgac tctccgcacc atcaacaata agctctcgcg attaggcgcc
    11701 gagccgttaa aagtcgctga cgaattggca actcaggatt taagcgagat catcaagagc
    11761 cgcatcgatc attttaaatc cagcggtagg atctaggcga tctcaggatt tgccggcgct
    11821 ttcacggtcg ctggatcgaa gccaagaccg tttgccgatc gtcacgcgcg ttggacgcgg
    11881 cggttgaagc ggatcgaatc gagaaaggcg gcaatgatga atcctgctcc gaccaggccc
    11941 gtgacagcat cagaaatatg ttgcaccgcg ttgataaaca tcatcactgc caaggcgatg
    12001 atggcataga atgcgccgtg ctccagataa cggtaagacg tgagcgtccc gcgctccact
    12061 agcatgatcg tcaggctgcg gacgaacatc gcgccgacgc ccaagccgat ggcaatgata
    12121 aatagattgt tcgacagcgc gaaggcgccg atcacgccgt caaagctgaa actggcatcg
    12181 agcacttcga ggtaaaggaa cgacgctgcg cctgactgca ccacttctcc agtcagtgtc
    12241 gattcgacct tcaacagcgc cgagatgcca tctactgcaa tgtaggtgac cagcccgaag
    12301 atccccgcgg ccagaaactg gaactgctcg gagccggcga gctgggtgga aacgaagtac
    12361 ataaggagca agacaagccc tagttccacc gcctcgatgc gcccgagccg cgttagaggt
    12421 cgctcaatga cggaaatcca gtgcacgctt ttttcccggt tggaaaagtg tcggagtccg
    12481 accatcccca ggaacgcgcc accgaaagcc gaaaccgaga catttgcgct agttagcacc
    12541 cgcgaatagt tgtccggatc ggtggccgcg agcaccagcg ccgaaacggg accgattcgc
    12601 gcgatgattg caacaataac caatggaaaa accatgcgca tgccaaagac agcgatggca
    12661 atgccccagg tgatgaaacg gcgccgccag agtggcgaca ttttcgttaa gacggcggca
    12721 ttaacgaccg cattatcgaa cgacagcgat acttcgaggg tggcgagcac ggcgacgatg
    12781 aacatcgtcg ccagcgcacc ggtccagctg tgattggttc tccagccaag gtatgccccg
    12841 agggcaagtc ccccgagtgt gaccaagaac gatttgcgaa agtaagtcac ggtcgctgtg
    12901 caatagcgta aaccgcggaa actttccagt ccttttggta ctttaggagt cagcgaccca
    12961 agatttaaac aaggttatcg ggagccgcat ggatcatttc acatccacgg taagattagc
    13021 ctactgtcac ggcgtttaga atccccatcc ccggtcaaac agggcgaaag gaatagcatt
    13081 gaattctaac ggaccagacg ccgaactatt agataatccg ggccagtcgt tttccctgca
    13141 tgatttcggc gtcgtggttg ggaaaagcca gatcaggccg ggccgccgtc gtcagggccc
    13201 gctcaagcgc aaccaccgta tcagccaaat tgtaatacca gccgacggga atgtgatcac
    13261 gaatattttt caacgtataa gcgatatcgc cgcaaagcac cgccttgccc cgtgctgtct
    13321 cgagcgccac catctgcgat cctggcgtat ggccgccggc tttaagcagc tccaatcccg
    13381 gataaagctc cacatcgcct tcgatcagtt cgattcgacc ttgctgtttt agcctttcca
    13441 gcgcgggacg caccctcggg cgaatctgct tggcaaagat atcgtgacgc atgagcggtc
    13501 ctgtccaaaa ctccaattcg gcgcgctgga tgacgaattt agcgttggga aaataatcca
    13561 agccggtgaa atggtcctgg tggaaatgag tcacgatgac tgtctttata tcctcggctt
    13621 taaccccaag ctgcgctagc aatgccggcg gctcaataaa attttttcct tgagcccatt
    13681 ccggcgcgac ataatcagga tgatatccgg tatcaacggc cacaggtcct tggggaccgt
    13741 caaccaacca gaaaatataa gcaaccggtt tatcccgctc gagatcgctg cgcacgcacg
    13801 gcataattcc cgcacgccac cagttttcac tcatcccgac catcaacgca tgaattgaat
    13861 gatttgatgt catagaactc ccttggtctc tatgaaagcc agattgctga catcactgga
    13921 cggctacctt cagctggatc gtagcacatc catctcgtct gtgacttttc ttgcagcacg
    13981 cttttctatc acggttcgga attttgtcgt agcaataaaa aaccgcggcc ggagcatccg
    14041 gccgcggttt gccgcaacgg actaacccgc ttacggcagg acaacagaat cgatcacgtg
    14101 gatgacgccg ttgctcgcca taatatctgt cgccgtcaca cgagcgttat ctatcatcac
    14161 tttgccgtcc atgctgctga tctgaagcgc gcgcccgttg attgtcttcg cgctgcttaa
    14221 gtttaccact tcaccagcgg ttactcggcc ggggaccacg tgataggtca gcaccgccgt
    14281 gagcttgtct ttgttttcgg gcttgagcag gttttccact gtgcccgcgg gcagctttgc
    14341 aaatgcctca tcggtcggcg cgaataccgt caacgggccc ggccccttca gcgcttcgac
    14401 cagtccagcg gcttgcgccg cggcaatcaa cgtcttaaac gatcccgccg aagcggcgac
    14461 ctcgacgatg tccttgtcca tcgattcagc cgtcgccaaa tcggtgactc ccaacgccag
    14521 cgccaatacc atcaatgcag ttttgaagac tctcattttt ttcctccttt ttatttttta
    14581 cgaatcttcg aattctgaca tgagcaaccg aggtgccaag gtaaagtatc attgctctgc
    14641 ggctctttag gccgtagtag tgacaagtag ataaagaagc gcgacctcgc tgagacaaca
    14701 acctgtctca gcgggtctca acgatgtctc gactgaaaca ggtgagacta tttttcttcg
    14761 ggactgattt tgaggtcgga taaacggcgg taaagcgtgg cc
//
LOCUS       DASUVS010000482        13908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1308127, whole genome shotgun sequence.
ACCESSION   DASUVS010000482 DASUVS010000000
VERSION     DASUVS010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13908
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308127"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..598)
                     /locus_tag="VF182_25310"
     CDS             complement(41..598)
                     /locus_tag="VF182_25310"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX6440462.1"
                     /translation="MKKVFLALVSWAVISSLAGVATVAAAPRDGAHEVQVSGGFFHAE
                     DTDFGTLNLDLSYGYYLTPGWQLGFRQALNYLFVDDASDQWTATTAPFINYHFRVTDI
                     IVPYLGAFIGAAWNDRDITGTLGPQLGVKFFVHEQTFLNLGYRYEWFWDKFERINNES
                     DNGNHIFNIGVGFTWGGSGAGTRRP"
     gene            complement(861..1010)
                     /locus_tag="VF182_25315"
     CDS             complement(861..1010)
                     /locus_tag="VF182_25315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX6440463.1"
                     /translation="MLWTIFVVLLVLWALGFVSGYAIGGFIHLLLVIAVVVLLIQLIQ
                     GRSSI"
     gene            complement(1094..2536)
                     /locus_tag="VF182_25320"
     CDS             complement(1094..2536)
                     /locus_tag="VF182_25320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX6440464.1"
                     /translation="MLRPEVIFGGVVGAAQLSDTEAAHRGLVTTAYFLPPLLLYVSDT
                     SLGGASIALRRSGKSVKLRAFLRVFKPLRIRVSQFAHLLFLKYYENMLGLRAASLTYA
                     TLLSLVPLLAVTFSVLKAFGVQQLIGPFLTQALAPLGPNRIEITRRLIEFVDNTRVDV
                     LGAVGVAGLFYSVIYLVSTVEEALNQIWRAQPSRTWTRRYGEYMGLLLVGPVVVFAAF
                     AVIASAQSYWLVQRLVDTTVFGLRFALLTRFLPFLFLSAAFAFLYKFMPSTHVALISA
                     LVGAALAALLWQLLSIVFTAFVVNSVSYAAIYSGFAILVIFLVWLYAAWLVILVGAQV
                     GYLHQHSSVYLPGAAAGKQGHLFEEHVALSLLCEIGQRFITGKPPATEKNLSRALGVP
                     PDQLESLLEKFIGRGLLLRSAEPAGVSLARPPEQISATEILDVLHGAANSDRDNGDAV
                     AEFLRRREAIVHESLAAVTLKDLADQHRSA"
     gene            complement(2658..8234)
                     /locus_tag="VF182_25325"
     CDS             complement(2658..8234)
                     /locus_tag="VF182_25325"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6440465.1"
                     /translation="MTESLAYTIVNVDDDDGGRYAKSRILRWAGYRVLEASTGADGLG
                     LVAEVKPQLVVLDINLPDMSGLDICRIIKGSPLTSDTLVLQISAAQITAEDRMFGLEA
                     GADAYLTGPVQPDELVATSRALLRLYNRERENRELLAALRDSEAQFRASFDLAGVGKT
                     QIDVATGKFIRVNNYYCELVGYTESELLGLKPSDLIHPDDRGREAELIAALLRGDTEE
                     LIGEKRLLRKDGRILWVSITAKLIRDASGAPERTISVVVDISEGKRAEERARRQNERL
                     ALLAQVSEQLLTAHDPALMIRQVFETVRQHLALDTYFNYRMDGSGSALVLESRGDADP
                     DVLPASQPLHISKALDDVPAHLRHGIILEALRKVDLQQARSAQARGFSAYAGHPLLVA
                     ERLIGTLCFASKRRDGFDNEELDFMRTVCHYVAMAQERLRLLAESRQQVERLEQSESR
                     LRLAVDGAGMGIWDVDLATDTLVWNRELYSLLGYAPDAGPVTRQMFRSRIYPEDFPGV
                     AQAIELAKSSRSLYCVEHRIIRADNGEIRWLAPFGRFLYDSAGAAVRFLGIVFDFTDR
                     KRAEDAFHETAERLRLAQVSAHLGIFDWNARTNDLAWTPEQEMVYGVPARGMTHYEHW
                     RKLVHPDDLARVEAERAQAIRQRQPFTLEYRILHPSGKTHWVASKGQGWYNAGGELVR
                     ILGVNIDITETKRAEEELRHQKQLLETITDNAPSMLFMTDPTGCVTFANPAATHLTGY
                     DEQELIGQILHEKIHHSRPDGSPYPMSDCRLHSVLPFEERITGHEEMFVRKDGTFFSV
                     RCAASPIIQDGMPVGTVIEAQDITKEKKAERALRDSEERMRMLSNHVPVFVWACRADG
                     WCYSLNDRWYEYSGFTPEQALGVGWLNAIHPEDRELAVSNWDQAVARGDGYEMELRCR
                     RHDGEYRWFIAKALPVKDASGLITDWIGTSLDIHDRKSWEQSLAHLAAIVTSSQDAVI
                     SFSRDSEIVTWNRAAEKLFGWSAEEALGQSAAIYVPPERSAESAQIAESLLHGVAIDQ
                     LETVRQRKDGSRVDVSLTISPIKFDQEVIGVSVTARDVTERKRIEQQREQQSRLLDLS
                     LDAISVWSVTQGIEYWNEGAEKLYGYSAEEAIGRSMQDLLKTSQSVSLQEIEDQIRRE
                     GEWEGRITHVSKGGGQVAVLSRQQRIARPDGEVILEVNRDITILEEAEQAVAEAAAHL
                     KAIVETAVDGIITIDENGFVASVNPAVEKMFGYSARELIGRNLSMLMAEPYRSEHEEY
                     LSHYGRTAEPKLIGTGREVRGRRKDGSEFPVDLGISETMIGARRFFTGLMRDATNRKQ
                     VEQALIDAKEAADTASYAKSEFLANMSHEIRSPLTGVLGYAEILLSRLKDANDLECVR
                     TIKQSGQYLLQIINDILDLAKIEAQGVELDRETIYLPAFLSEVYALMEVNAKEKSLPL
                     FLKYEGFIPQTIESDSKRLRQILINLLSNAIKFTDHGKVELAMRFLRAESQLQFEVSD
                     SGIGMTEEQQRTLFHPFTQGDSSVTKVYGGTGLGLAITKRLVGALGGVIAVESVSGRG
                     STFRVTIPIQLVSGSSYRSSMVQESRGVAHLFAGKMPARVLICEDQPEIRRLMGWLIE
                     EAGGRATLIDSGEAAVDVVQQAADDFDVILLDIQMPRLDGYEVARRIRQLGFRKPVIA
                     VTAAAMPTDREKCLQAGCDDYLPKPIEVDKLLSMIARYTAKLASETQASPETPPAEES
                     LPVSSGPTNWRILVVDDRPVALNATSRLLEMEGHTVRSASTGHAAIRIAREFAPDLVL
                     LDISLPDISGYEVFRRLKASDSLARTVFVALSGHGYEERIRARQVGFDDYLMKPFDIK
                     EVDRLIAVNLVQERQL"
     gene            complement(8276..10504)
                     /locus_tag="VF182_25330"
     CDS             complement(8276..10504)
                     /locus_tag="VF182_25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6440466.1"
                     /translation="MSYSIINVRIEYEHDVVLARQRAREIAALLGFDIQDQTRIATAV
                     SELARNAFVYAGRGNVEFEIEGRTVPQVLVIRVGDSGPGIANLGEILEGRYASRTGMG
                     LGLIGARRLVDQCEIHSDPKKGTKVTLRKIMPRHAPLVTTLSLRALVDQLAQRRSPGP
                     LEEIQQQNRELVRALAELRERQEELARINRELEDTNRGVVALYAELDEKASHLRRADE
                     MKSRFLSNMSHEFRTPLNSILALSGLLLDGADGDLTGEQDKQVGFIRKGAETLLELVN
                     DLLDLAKIEAGKVEVQPVDFEVSNLFSALRGMLRPLLVTQSVGLVFEDPGEIPCLYTD
                     EGKLSQILRNFISNALKFTERGVISVSAMFNKADQTVTFAVSDTGMGIPAEDQAKIFE
                     EFVQLDNPAQRKIKGTGLGLPLCRKLATLLYGKIDLVSQIGIGSTFSVTIPLYYSQAP
                     ESQTVREVMPAASWQLDETRIPVLVLEDAPETRLLYEKFLRQSPFQLIPANNLGEARR
                     ILGQVRPRAIVMDILLRGEDAWTWLVELKAESATREIPVLVITSVDDERKGLSLGAAA
                     YCLKPIGRATLIEQLQALTNSEISGAERSLQTGNAQPCVLIIDDEAVDRYILVRLLEQ
                     QPCIIEQAASGLEGLKILREIKPDLIFLDLNMPGFSGYEVLNQIKADPPTRNIPVAVV
                     TSAELGAPQRKELEGRVYALINKVDLSAKAVDDLLAAARENGGRAGTASPGADFEANS
                     NG"
     gene            complement(10618..11646)
                     /locus_tag="VF182_25335"
     CDS             complement(10618..11646)
                     /locus_tag="VF182_25335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015205500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding SpoIIE family protein phosphatase"
                     /protein_id="HEX6440467.1"
                     /translation="MIPVWERQKTFEISEPSHVAEVRRFTSQLAGATGFNEAQAGAVA
                     LVTTELATNLLKHASSGEIVVRPIVERGIAGVELLALDQGSGIANLAESLRDGYSTAG
                     SPGTGLGAITRLAAEFDIQTQPGKGTAAMARLWSAKLDGPDDNIAFGAICKPMRGETR
                     CGDGWGCELLADRCILALVDGLGHGTGAAAAAELAMASVRQHRAKAPASIVQYAHDAL
                     RATRGAALAVAEINWAQQSVRYCGIGNISATIIAGDTLRHLVSYNGTAGHEARKIVEF
                     TYPWNANALLVMHSDGLMTRWNLQAYPGLLQRHPSLIAGVLYRDFVRGRDDVTVLAAK
                     GAVPRSVG"
     gene            complement(11643..12053)
                     /locus_tag="VF182_25340"
     CDS             complement(11643..12053)
                     /locus_tag="VF182_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012605601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEX6440468.1"
                     /translation="MVVLKDETAPIRSSDDIVRVRRIVREWAVSLGFSLVDQTKVVTA
                     ASELARNVIDHGGGGDFLIESLNNDARRGLRLTFSDEGPGILDMDQAMKDGFTTGGGL
                     GLGLGGAKRLVNEFLIDSKPGRGTTVVVIRWKNL"
     gene            complement(12034..12423)
                     /locus_tag="VF182_25345"
     CDS             complement(12034..12423)
                     /locus_tag="VF182_25345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6440469.1"
                     /translation="MERIPILKMGRFLLVTIQVDMHDRLAMALQEDLTERIVKDHARG
                     VLLDISALEIVDSFIGRMIGHIAGVSRVLDAKTVVVGMRPAVAITLVELGMTLEGVST
                     ALNVDSGMAMLSASLEEREQLNGGPQR"
     gene            complement(12432..13292)
                     /locus_tag="VF182_25350"
     CDS             complement(12432..13292)
                     /locus_tag="VF182_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6440470.1"
                     /translation="MTEQQSRVAQLLRKHERDVLQQWLSYQISATTMRADLIKESDLR
                     EQSRRFLQLFTDVVQQNGHDENTGSWKSVKDMLGEVSRGRVAQGFSPSETATFVFSLK
                     QPLFDMLRRELRDAEELSREIWITTALLDKLGLYTTEAYLQSRESIISRQQQELMELS
                     TPVVQLWDDILALPLIGTLDSERTQVVMESLLEKVVETGASIAIIDITGVPTVDTLVA
                     QHLLKTVAAARLMGADCIISGIRPQIAQTIVHLGINLADIITKATLADAFAIALQRTG
                     AKVTRLTGKE"
     gene            complement(13688..>13908)
                     /locus_tag="VF182_25355"
     CDS             complement(13688..>13908)
                     /locus_tag="VF182_25355"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6440471.1"
                     /translation="NAPLNQWLQEKEKEYLAQKLDDFGGNVGLTAKSCRIGVRTLSRK
                     MRAYGLDKKLFKQKDDAAKAISPKTGQA"
BASE COUNT         3125 a         4059 c         3688 g         3036 t
ORIGIN      
        1 ccttttcaga ttgacgccgg gttttggttt tggaagaagg ttaaggtctt ctcgtgcccg
       61 cgcccgaacc accccacgta aatccaacac cgatgttgaa tatgtgattg ccgttgtcgg
      121 attcattatt aattcgctcg aatttatccc aaaaccattc gtagcggtag ccaagattca
      181 agaaagtctg ttcatgaacg aagaatttga cgcccaactg agggcccagg gttcccgtga
      241 tatcgcgatc gttccacgcg gcaccgatga aagcgccgag gtacgggacg atgatatcag
      301 tcactcgaaa atggtagtta ataaagggtg ccgtagtggc ggtccactga tcgctggcat
      361 cgtctacgaa gagataattg agcgcctgtc gaaaccctaa ttgccatccc ggggtcaagt
      421 aataaccata actcaaatcc aagttcaaag tgccgaagtc agtatcttct gcatgaaaaa
      481 acccgcccga aacttgaacc tcatgggccc cgtcccgcgg tgccgcagca actgtcgcga
      541 ctccagcgag cgaactaatc accgcccaac tcacaagagc caggaaaact ttcttcatag
      601 gaatacctcc actaataaat ttgaagcttt tgtgagcaat gaagatgcca actgcgcgaa
      661 atacaagcgc gttgtaaata aacaattatt ggcactatag actttggggt gtccggcaaa
      721 cgctactctg ttgtcctacg cctgtagcgt cagcgctaca ccccaaccgg gtaaattcct
      781 gctctgacaa aatctaaata atttgaacct caggcgcagc gtgcgtatcc agatagtaac
      841 ggctacgtca ggtgatcgtc tcagattgag ctgcgccctt gaatcaactg aatcaacaac
      901 accactacgg cgattaccaa tagcaggtga atgaatccgc caatagcgta gccgctgaca
      961 aatcctaacg cccataaaac cagaagtacc acgaatatag tccaaagcat gatcggctcc
     1021 ttgattgtag tctctgttgc atcctcgacc gcaacagtgc acacgacatg ccaatcgacc
     1081 gtcgcgacac cagttatgct gaccggtgtt gatccgctaa gtctttaaga gtgaccgcag
     1141 ccaggctttc gtgaacgatt gcttcgcgcc ggcgcagaaa ttctgcgact gcgtcgccgt
     1201 tatctctgtc ggagtttgct gcgccatgta gaacgtcgag tatttccgtt gccgatattt
     1261 gttcgggcgg tctggctaga ctgacccccg cgggttcggc cgatctaaga agcagaccgc
     1321 gaccgatgaa tttctccagc aaactctcga gctgatccgg cggcaccccg agggcgcggc
     1381 ttagattttt ttctgttgcg ggcggcttgc cggtaatgaa tcgctggcca atctcacata
     1441 gcagagataa tgctacgtgc tcctcgaaca ggtgaccttg ctttcccgcg gcagcgccgg
     1501 gaaggtaaac cgaggagtgt tgatgcaggt atccgacttg ggcgccgacc aagatcacca
     1561 gccaggccgc gtacagccac accagaaaga taactaagat tgcaaaaccc gaatagatcg
     1621 ctgcatagct gaccgaatta actacgaatg cagtaaaaac aatgctcaac agttgccaga
     1681 gaagtgctgc cagcgcggca ccgactagag ctgaaatcaa agccacatgc gtgctgggca
     1741 taaatttata aagaaatgcg aatgccgccg aaagaaacaa gaatggcaga aagcgcgtta
     1801 gcagcgcgaa tctgagtcca aagacggtgg tatcaactaa tcgttgaacc agccagtaac
     1861 tctgcgccga ggcgatcacg gcaaaagcgg caaagacaac aaccgggccg accagcagca
     1921 gccccatgta ttcaccgtac cggcgcgtcc aagtgcgcga gggctgtgct cgccagatct
     1981 gattaagtgc ttcttcaaca gtgctgacca gatagatcac tgaataaaac aggcccgcga
     2041 cgccaaccgc gcccaaaaca tcgactcgcg tgttgtcaac gaattcgatc aagcgccggg
     2101 tgatttcgat tcgattcggt ccgagaggag caagcgcctg agtcagaaat ggcccgatca
     2161 gctgctgcac gccgaaggct ttgagcaccg aaaacgtcac cgcaagaagc ggcaccagcg
     2221 atagcaacgt tgcatacgtt aagcttgccg cccgcaagcc caacatattc tcgtagtact
     2281 tcaggaacag cagatgggca aattgcgaga cacgtatccg aagcggttta aatacccgca
     2341 aaaaagcgcg cagtttcacg cttttgcccg atctacgtag ggcaatcgag gcgccgccca
     2401 gcgaagtgtc agaaacataa agcaacagag gcggcaaaaa atatgccgtt gttactaatc
     2461 cgcggtgtgc agcttcagtg tcgctaagtt gagcggcgcc aactactccg ccgaagatga
     2521 cttcgggcct gagcaatcgg cctcaagagc gtcgctcgca cgatggggat tgatctgtcg
     2581 accaggagag ggcgaatggc gggtcaagcg aactgcagga aagatatgag gaatgagatc
     2641 gatttaacag tctggcttta caattgacgc tcctggacta agttgacggc aatcagcctg
     2701 tcaacttctt tgatatcgaa aggtttcata agatagtcat caaagccgac ttggcgagcg
     2761 cgaatacgct cttcgtaacc gtgacccgat aaggcgacga acacggttcg cgcgagggag
     2821 tcactcgctt ttaaccttcg aaaaacctca tacccgctaa tatccggcag gctgatatcc
     2881 aacagtacaa gatccggcgc aaactctcgt gcgatgcgaa tcgcggcatg cccggtcgac
     2941 gcgctgcgaa ccgtatgacc ttccatttca agcaagcggc tagtcgcgtt aagcgccact
     3001 ggacgatcat cgaccactag gatgcgccag ttagttggcc ccgaagaaac cggtagcgac
     3061 tcctcggccg ggggagtctc cggagacgcc tgcgtttcac tggccagttt ggctgtgtag
     3121 cgcgcgatca tcgataacaa tttgtcgact tcaatgggct tcggcaggta gtcgtcgcaa
     3181 ccggcctgta agcacttctc gcgatccgtt ggcatggcag ccgccgtcac cgcgataact
     3241 ggtttgcgaa atcccaattg ccggattcgc cgtgcaacct cataaccgtc gaggcgaggc
     3301 atttgaatgt ctaataggat gacatcgaaa tcgtcggcgg cttgctgaac cacgtccact
     3361 gccgcttcgc cgctatcgat taaggtcgcc cgaccaccgg cctcttcgat cagccagccc
     3421 atgagacgac ggatctccgg ttggtcttcg cagatcaata cccgcgcagg catcttgccg
     3481 gcaaacaagt gcgccacacc ccggctctcc tggaccatgg aagagcgata ggatgaaccg
     3541 gagaccagtt ggattggaat cgtcacccgg aaggtgctgc cgcgaccgga tacactctca
     3601 accgcgatca caccgccgag cgcgccgacc aaacgtttgg taattgccaa tcctaggccc
     3661 gtaccgccat agactttggt taccgaacta tcgccttgtg tgaaggggtg aaacagggtt
     3721 cgctgctgtt cttcggtcat accaatgcca gagtcgctga cctcaaactg cagctgcgac
     3781 tccgcgcgca gaaaacgcat cgcaagttcg accttgccgt gatcggtaaa tttgatcgca
     3841 ttgctcaaca agttgatcag aatttggcgc agtctttttg aatcgctctc gattgtttgt
     3901 ggaataaatc cttcgtattt caggaacaga ggcaacgact tctctttggc gttgacttcc
     3961 atgagcgcgt aaacttcgct taagaaagcc ggcagataaa tcgtctctcg atcaagttct
     4021 acgccctggg cctcaatttt ggcgagatcg agaatgtcat tgataatttg caacaggtac
     4081 tgaccgctct gcttgatcgt gcgcacacat tcgagatcgt tggcatcctt gaggcggctg
     4141 agtaagattt cagcgtaccc taaaactccg gtcagcgggc tgcgaatctc atgactcatg
     4201 ttggcgagga attcactttt ggcatagctt gccgtgtcgg ccgcttcttt ggcgtctatc
     4261 aacgcctgtt ccacctgttt ccggtttgtg gcatcacgca tcagtccggt gaagaaccga
     4321 cgcgcgccga tcatcgtctc gctgatcccc aaatcaaccg gaaactcgct accgtccttg
     4381 cgccggcccc gcacctcgcg gccagttcca atcagtttgg gttccgccgt tcggccatag
     4441 tgactcagat actcttcgtg ttcggacctg tagggctcgg ccatgagcat tgagagattg
     4501 cgcccgatca gttcgcgcgc cgaatacccg aacatcttct cgaccgccgg attaaccgag
     4561 gccacgaagc cgttctcatc gatggtaatg atgccatcga cagcagtctc aacgatcgcc
     4621 tttagatgag cggccgcctc agctaccgcc tgctccgctt cctcaagaat ggtgatgtct
     4681 cggttaacct cgagaatcac ttcgccatcc ggccgcgcga tccgctgttg acggctgagg
     4741 accgcgacct ggccgccgcc tttgctaaca tgggtgatcc gcccttccca ttcgccctca
     4801 cggcgaatct gatcttcgat ctcctgcagg gacaccgact ggctggtttt taacagatcc
     4861 tgcattgacc ggccgatggc ctcctcggcg gaatagccgt agagtttttc agccccttcg
     4921 ttccagtact cgataccttg ggttacgctc cagacgctga tagcatccaa cgatagatct
     4981 aacagccgcg attgctgctc gcgctgttgt tcgattctct tgcgctcggt gacgtcgcgc
     5041 gcagtcacag acacaccgat gacctcctga tcgaatttga tcggtgagat ggttaaagag
     5101 acgtcaaccc ggctgccgtc cttgcgttga cgtaccgtct ccaactgatc gatggctacg
     5161 ccgtggagca agctctcggc tatttgcgca gattcagccg aacgttcggg gggcacataa
     5221 atcgcggcgc tttgacccaa tgcttcctcg gccgaccagc caaaaagttt ctctgccgcc
     5281 cggttccatg taacgatctc tgaatcgcgt gaaaacgaga tgacggcatc ctgcgatgag
     5341 gtgacgatgg cggcgagatg agcaaggctc tgctcccatg atttgcggtc atgaatatcc
     5401 aggctagttc cgatccaatc cgtgatcagg ccgcttgcat ccttgaccgg aagagctttt
     5461 gcgataaacc agcgatattc gccatcgtgg cgccggcaac gcaactccat ctcgtatcca
     5521 tcccctcgag caacagcctg atcccagttg gagaccgcca gctcccgatc ctctggatgt
     5581 atcgcgttca accagcctac accaagcgcc tgttccggag tgaagccgga gtattcgtac
     5641 cagcggtcat tcagcgaata gcaccagcca tcggcgcggc aagcccatac gaatacaggc
     5701 acatgattgg acagcatgcg catccgttct tcactgtcac gcagcgcacg ctcggctttt
     5761 ttctctttag tgatgtcctg ggcttcgatc acggtgccga cgggcatgcc gtcttgaata
     5821 atcgggctgg cggcgcaacg cacagaaaaa aatgtgccat ccttgcgcac gaacatctct
     5881 tcatgaccgg taattcgctc ctcaaacggc aacacgctat gaagccggca atcgctcatt
     5941 gggtacggcg agccgtcggg ccggctgtga tgaatttttt catgcagaat ctggccgatc
     6001 agctcttgct catcataacc ggtcagatgg gttgccgcgg gattggcaaa ggtaacgcaa
     6061 cccgtcgggt cggtcataaa caacatcgat ggcgcattat ccgtaatcgt ctctagcagt
     6121 tgcttttgat ggcgcagctc ttcttcggct cgcttggttt cggtgatgtc gatgttaacg
     6181 ccaagaatgc gaactagctc gccgccggcg ttgtaccaac cctggccctt ggaagcgacc
     6241 cagtgagtct ttccggaagg atgcagaatt ctatattcca gagtgaaggg ctggcgctgc
     6301 cgaatggcct gggcgcgttc agcctccact cgcgcaagat cgtccgggtg aaccagcttt
     6361 cgccagtgct cgtaatgggt catgccgcgc gccgggactc catagaccat ctcctgctcg
     6421 ggcgtccatg ctaaatcgtt ggttcgcgca ttccagtcga agattccaag atgggcgctt
     6481 acctgcgcca aacgaagccg ctcagcagtc tcatggaagg catcctcggc gcgtttgcgg
     6541 tcggtgaagt cgaagacgat gccaaggaaa cgaaccgccg cgcccgcgct atcgtagaga
     6601 aaccgaccga aaggcgccag ccagcgaatc tcaccattgt cggcgcgtat gatgcggtgt
     6661 tcaacgcagt atagtgaccg ggacgactta gccagctcga tcgcttgcgc cacaccgggg
     6721 aaatcctcag ggtaaatgcg cgagcggaac atttggcggg tgacgggtcc tgcatccgga
     6781 gcatatccca gaagtgaata cagctcccgg ttccaaacca aggtatccgt tgccaaatcg
     6841 acatcccaga ttcccatccc ggcgccgtcg actgccagcc ggagcctgct ttcgctttgc
     6901 tccaggcgct ccacctgctg ccgtgactcc gccagcaggc gcaagcgttc ttgcgccatc
     6961 gccacgtaat ggcaaaccgt gcgcatgaag tcgagttctt cgttatcgaa gccgtcgcgt
     7021 ctcttgctcg cgaaacaaag cgtcccgatc aagcgttccg caaccaacag agggtgaccg
     7081 gcataggccg aaaagccgcg cgcttgggca gacctcgcct gttgaagatc gaccttcctg
     7141 agcgcttcca ggatgatccc gtggcgtaaa tgagcaggaa cgtcatcgag cgctttgctg
     7201 atatggagtg gctgagaggc aggcaataca tcagggtccg catcgccccg ggattccaag
     7261 accagggcag agccggagcc gtccatccga taattgaagt aagtatccaa tgctaggtgc
     7321 tgccgcacag tctcgaaaac ttgtcggatc attagagcgg gatcatgcgc ggtaagcagt
     7381 tgctccgaca cctgcgcaag caacgccagg cgttcgttct gccggcgcgc tcgctcttca
     7441 gcgcgcttgc cttcgctgat gtcgacgacg accgaaattg tccgttcggg cgcgccgctg
     7501 gcatcgcgga tcaatttggc agtgatcgag acccagagaa tgcgcccgtc tttgcgcagc
     7561 agacgtttct cgccgatcag ttcctcggtg tcgccgcgca ataatgccgc aatcaattcg
     7621 gcttcgcggc cgcgatcgtc gggatgaatc aggtcggagg gtttcaagcc cagcagctct
     7681 gactcggtat aaccaaccag ctcacagtaa tagttattga cgcggatgaa ttttccggtc
     7741 gctacgtcga tctgcgtctt gccgacgccg gcgagatcga aactggcgcg aaactgcgct
     7801 tcgctgtccc gtaacgccgc gagcagttcg cggttttctc gttcacggtt gtacagacgc
     7861 agcagcgccc gcgaggttgc gaccaactcg tcgggttgta ccggcccggt caggtaagca
     7921 tcggcgccgg cttccaaacc aaacatgcgg tcttctgccg taatctgcgc agctgaaatc
     7981 tgcaaaacga gagtatcgga ggttaaaggg ctgcccttaa tgatgcggca aatatccagt
     8041 ccgctcatat ccggcaggtt gatgtccaga acgaccagct gcggttttac ctcggccacc
     8101 aaacccagac catcggcccc ggtggaagct tccaaaaccc ggtagccggc ccagcgcagt
     8161 atccggctct tggcataacg cccgccgtca tcgtcgtcta cgttgacgat cgtataagcc
     8221 aacgattcag tcatgagcta tctcacgcca aggtggctag gtatcggcgc gattttcagc
     8281 cgttgctgtt tgcctcaaaa tcggcgcctg gagaagccgt gcctgcacgg ccgccgttct
     8341 cccttgctgc cgcaagcaaa tcatcgacgg ctttggccga gaggtcaacc ttatttatca
     8401 gcgcataaac ccttccttcg agttctttgc gctgaggagc gccaagctcc gccgatgtca
     8461 ccacggcgac gggaatattg cgcgttgggg gatcggcctt gatctgattc aaaacctcgt
     8521 agcctgagaa ccctggcatg ttgagatcca aaaaaatcaa atccggcttg atttctcgca
     8581 gtatctttaa accttccaac ccgcttgccg cctgctcaat gatgcatggc tgctgctcga
     8641 gtagccgcac gagtatatag cggtccactg cctcgtcatc gataatcaag acgcagggct
     8701 gcgcatttcc agtttgcaaa gatcgctctg ctcccgagat ttccgaattt gtaagagcct
     8761 gaagctgctc gatcaaagtg gcgcgtccga tcggcttcag acagtaagct gcggcgccca
     8821 gggataagcc cttgcgttcg tcatcgacac tggtgatgac cagcaccggg atctcccgcg
     8881 tcgcgctttc ggctttgagt tcgaccaacc aggtccatgc gtcttcaccg cgcagaagaa
     8941 tatccatcac aatggcgcgc ggccgcacct ggcccaggat acggcgggct tcgcccaagt
     9001 tgttcgcggg gatgagttga aatggcgatt gacgaagaaa tttttcatac aggaggcgag
     9061 tctccggagc gtcctctagg accaacactg gaattcgcgt ttcatcaagc tgccatgacg
     9121 ccgccggcat cacttcgcgc acggtttggc tctccggcgc ctgagaatag taaagcggta
     9181 tcgttactga aaacgttgaa ccgattccga tctgactcac cagatcgatt tttccataca
     9241 gcagtgtagc cagcttgcgg cataacggaa gacccaggcc tgtacccttg atcttccgtt
     9301 gggccggatt gtcgagctgg acaaattctt caaagatctt cgcttggtcc tcggccggga
     9361 tacccatgcc ggtatccgag accgcaaatg tcaccgtctg atcggcctta ttgaacattg
     9421 ctgaaaccga gatcactccc ctttccgtga attttaacgc gtttgaaata aaattgcgca
     9481 aaatttgcga aagcttgcct tcatcggtat ataagcaagg gatttcgccg ggatcctcga
     9541 acaccaagcc aacgctctgg gttaccagaa gcgggcgcaa catgccgcgc agtgcgctga
     9601 aaagattgga cacttcgaag tcgaccggct ggacttcgac cttgccggct tcgatcttag
     9661 ccaaatccaa aagatcgttt accaattcca gcagcgtctc cgcgcctttt cgaatgaagc
     9721 cgacttgttt gtcttgctca cccgtcaaat ccccgtcggc accgtccagc agcaatcccg
     9781 atagcgccaa gatcgaattc agcggagtgc gaaactcgtg gctcatgttg gagagaaatc
     9841 gtgacttcat ttcgtcggcg cgccgcaagt gactcgcctt ttcatctagc tcggcataaa
     9901 gcgcgaccac gccacggttc gtgtcctcga gctcccgatt gattcgcgcc aactcttcct
     9961 gccgctcgcg caactctgct aaagcgcgca ccagctcccg attttgctgt tggatctcct
    10021 ccagcggacc cggcgatcgc cgctgcgcca attgatccac caaagcgcgc aacgacagcg
    10081 ttgtcaccag cggagcatga cgcggcatga tttttcgaag ggtgaccttg gttccttttt
    10141 tgggatcgct gtgaatctcg cattgatcga ccaagcgtcg ggcgccgatc agccctaacc
    10201 ccattccggt gcgcgatgca taacgccctt ccaaaatctc cccgagattt gcgataccag
    10261 gaccgctgtc gccgacgcgg ataaccaaaa cctggggcac ggtccggcct tcaatctcga
    10321 actccacgtt gccgcggccg gcataaacaa aagcgttgcg tgccagctcg gaaaccgcag
    10381 tggcaatccg cgtctgatct tgaatatcga agccaagcag cgcggcgatc tcgcgcgccc
    10441 gctgccgcgc caagacaacg tcgtgctcgt attcgattct aacgttaata atcgaatagc
    10501 tcatagcaat cctgtttgta gttttcgtcg cagttcttag ctcgattcct tgaacaagac
    10561 gcgagtaaac agctccaggt tccgacactt aagtccaggg gcgagctgaa aacattttta
    10621 cccgacactc cgcggcacag ctcctttggc ggccaggacg gtgacgtcat cgcggccgcg
    10681 gacaaagtcg cgatagagga caccggcgat aaggcttgga tgtcgctgaa gcaggcccgg
    10741 ataagcctgc agattccagc gcgtcatcag tccgtcggag tgcatgacca atagcgcgtt
    10801 tgcgttccat ggataagtga attcgacaat cttgcgcgct tcgtgaccgg cggtgccgtt
    10861 gtaagaaacc agatggcgca gggtgtctcc agcgataatc gtggcagaaa tgtttcctat
    10921 gccgcaataa cgaacagatt gctgagccca attgatctcg gccaccgcga gagccgcacc
    10981 gcgcgtggcg cgtagcgcgt cgtgggcata ttgaacgatg ctcgccggag ctttggcacg
    11041 gtgctggcgg acgctggcca tggccagttc ggctgccgcc gcagcacccg ttccgtgacc
    11101 caggccatcc accagcgcca aaatgcatcg atccgcgagg agctcgcagc cccatccgtc
    11161 accgcaacgc gtctcgccac gcatcggctt gcagatcgcg ccgaacgcga tgttatcgtc
    11221 cgggccgtca agcttggcac tccagagccg ggccatggct gccgtgccct tccccggttg
    11281 cgtttgaatg tcgaattcgg cagccagccg ggtgatggcg ccgagaccgg tgcccggaga
    11341 gccggccgtg gagtagccgt ctcgcagaga ttcggcaaga ttcgcaatcc ccgacccttg
    11401 gtccagggcc agtagttcga cacccgcgat gccgcgctca acgatcggcc ggacgacgat
    11461 ctcaccgctg ctcgcgtgct tcagcaggtt ggttgccagt tcggtagtga cgagcgcaac
    11521 ggcgcctgcc tgtgcttcat tgaagcccgt tgctccggcc aactggctag taaaacgtcg
    11581 cacctcggca acatgactgg gttcgcttat ttcaaaggtc ttttgccttt cccaaacagg
    11641 gatcatagat ttttccagcg gatgacgacc accgtcgtgc ctcggccggg tttcgaatca
    11701 atcaggaatt cgttaaccag gcgctttgcg ccgcccagcc ccagacccaa cccgccgccg
    11761 gtggtaaacc catccttcat tgcctgatcc atatcaagaa tacccggtcc ttcatcggaa
    11821 aacgtcagcc gcagcccgcg cctggcatca ttgttgagcg attcgataag gaagtcgcca
    11881 ccaccgccgt gatcgatgac attacgggcc aactcgctgg ctgccgtgac gaccttggtc
    11941 tgatccacca agctgaaccc cagcgatact gcccactccc ggacaatacg gcgcaccctg
    12001 acgatgtcgt cagaactccg gatcggagca gtctcatctt tgaggaccac cattaagttg
    12061 ttccctctct tccagcgatg cgctcaacat cgccataccg ctatcgacat ttaatgccgt
    12121 gctgactccc tcgagcgtca tgcccagttc taccagggta atggcaaccg caggcctcat
    12181 gcctaccacc acggttttgg catcgagcac gcgcgagacg ccggcaatgt gaccgatcat
    12241 ccggccgata aatgaatcga cgatttccag agcggaaata tcgagcaaca cgccgcgggc
    12301 gtggtctttg acgatgcgtt cggtcaagtc ctcctgaagc gccatcgcta gacggtcgtg
    12361 catatctacc tgaatagtga caagcagaaa gcggcccatt ttcaggatcg gaatcctttc
    12421 catcaatcac ctcattcttt gccggtcagg cgcgtcacct tggcgccggt tcgctgcagg
    12481 gcaattgcaa atgcatcagc caaggtcgct tttgtgatga tatcggccaa gtttatgcca
    12541 aggtgaacaa tggtttgagc gatctgcgga cgaataccgc tgatgatgca atcggctccc
    12601 atgagccggg cggcggcgac agtctttagc agatgttgcg caaccagtgt gtccaccgtg
    12661 ggcacgccgg tgatatcgat gatcgctata gaggcgccgg tttcgacgac tttctcgagc
    12721 aggctctcca tgactacttg cgtgcgttcg ctgtcaagag taccgatcag tggcagcgcc
    12781 aggatatcgt cccagagctg gaccaccgga gtggataact ccattaactc ttgctgctgg
    12841 cgcgagatga tcgattcgcg gctctgcagg taggcttccg tggtgtacag cccgagcttg
    12901 tcgagcagcg ccgtggttat ccagatctcg cgggacagtt cttcagcgtc ccgcaattcc
    12961 cgacgcaaca tgtcaaaaag cggctgcttg agtgaaaaaa cgaacgtcgc ggtttcagac
    13021 gggctgaaac cctgggcaac ccggccgcgc gatacttcgc cgagcatgtc tttgaccgat
    13081 ttccaggagc cggtgttttc atcatggcca ttctgttgca caacatcggt aaagagttgc
    13141 agaaaccgcc gcgattgctc gcgcaaatcg ctctccttaa ttaaatccgc tcgcatggtt
    13201 gtcgccgaga tctggtacga caaccactgc tgtaagacat cgcgttcatg ctttctaaga
    13261 agctgcgcca ctctcgattg ctgttctgtc atttgttcct ctctctttca tcttgacgtc
    13321 ggtttcaacc ttagcgagcg atcggtcaag cacctcccca gaaactcgat agttgaattc
    13381 cccacgcggg ttcaccatct ggttgagcgg ccaggtgggt ccttcccatg atgccctggc
    13441 gcagcttatc agcgaggaga agcgctttcg tgaaggcttc tgtcttggaa tggagcgatt
    13501 cagtcaatcc gggtgtaaca gaaagacctt ggccttatgt ctagcaaatt acgctggagg
    13561 tatacaaaca aataatcagc aaaggggcat agccagccgt ccgcacctcc ggccttaacg
    13621 aaagaccgat aaaagagatt tgtcggaact agaaggaaag aaggaacggt gagctaaact
    13681 gtggggatca ggcctggcct gttttgggcg agatagcctt tgcggcgtcg tctttttgtt
    13741 tgaacaactt cttatcgaga ccgtaagcgc gcattttacg agagagggtg cgcacgccga
    13801 ttcggcagct cttagcggtc aaaccgacgt tgccgccgaa atcgtcgagt ttttgcgcca
    13861 gatactcttt ttccttttcc tgcagccatt ggttcaacgg ggcgttga
//
LOCUS       DASUVS010000483         7727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_213148, whole genome shotgun sequence.
ACCESSION   DASUVS010000483 DASUVS010000000
VERSION     DASUVS010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7727
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213148"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..3666
                     /locus_tag="VF182_25360"
     CDS             322..3666
                     /locus_tag="VF182_25360"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6440472.1"
                     /translation="MHCLRCRHENPPGVKFCGECGARFEALCPSCKASNPPTNKFCGQ
                     CGATLGQVAAGSSFTSPKSYTPKHLAEKILTSKDVLEGERKQVTVLFADMKGSMELLA
                     DRDPEDARKLLDPILEHMIDAVHRYEGTVNQVMGDGIMALFGAPLAHEDHAVRACYAA
                     LRMQESVKHYAEGVRRTEGVPIQIRIGLNSGEVVVRSIGSDLKMDYTAVGQTTHLAAR
                     MEQMAMPGSILMTAHVLSLAESYVQVKPLGPVNVKGMSESVDLYEVTGAGIARTRLQA
                     AAGRGLTRFIGRDVETEQLRKALEQAREGHGQVVAVMGEPGVGKSRLFFEFTRSHRAE
                     GCLIIESGSVSYGKATPYLPVADLLKAYFKIHDRDTQRDVREKVTGKLLTLDKTLEPA
                     LPALLSLLDVPIDDPQWQNLDPLQRRQSTLDAIRGLLLRESHVQPLLLVFEDLHWIDS
                     ETESFLDSLIDSLPTAPLLLLVNYRPEYRHSWGNKTYYSQLRIDPLPPESAQQLLLAT
                     LGDDPSLQPLKQLLIERTEGNPFFIEESIRTLIETKVLAGDRGKYRLSGSSVITHVPA
                     TVHAVLAARIDRLALEQKRLLQSAAVVGKDVPFPLLQGIAELPEDELRCHLGHLQAAE
                     FLYETRLFPDLEYTFKHALTHEVAYGSVLQDRRRALHAGIVEAIERLYPDRLIEQVEL
                     LAHHAFQGELWEKAVTNLRQAGGKAADRSAHPEAVSYFERVLEALKHLPESRQKIEQA
                     IDVRIEMRSSLLPLGEQLKLFERMCEAEVLAETLHDQQRLGQVSAYLSGYFIQAGDDP
                     IRAVEKGQRALEISSSTKNFALQVQANHFLASAYHYLGDYDRALTHFRRNVDTLAGDK
                     IYERFGLAFLPSVGSRYQLVILLAERGQFDEAAIHGTEAVRIAETANHPFSLSTAYYA
                     VGYLYISKGAIDQAIGLLERELEFCRSWNLHQNLRRVGAALGYAYAAAGLDLKALPLL
                     DVAYERLRLASARAQLAQAYLLIGRREQADLIASQALDTSRSQGERGREAEALYVLGE
                     IGLSAIPVDIAGVQDHYRQAIALADEIGLRPLVAHCHVGLGKLYQRTGDLQRAREHLT
                     NGVAMMREMEMGLWLDRARAELEKLG"
     gene            3718..4716
                     /locus_tag="VF182_25365"
     CDS             3718..4716
                     /locus_tag="VF182_25365"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440473.1"
                     /translation="MTLGFDKLRLLFVIVLTIISPVSATAASAPHKAIFTFGGLNERS
                     GVLFVARDAGLFHKHGLDATVVNVRNAAVGMSALASGETQFHVGSATGSSIGAMAGGV
                     DLAFIAGLINKLDGTFVVAPKIRAPEDLKGKRIGVQSIGGGVWMFTMLAFEKWGLNPE
                     RDNIQFRVLGDQSVIAQAMTQGIVDGAYLGYTFGAQMERQGYRILADLAKIGVPYQGL
                     GVMARRNLVDRTPEVAEKALRAMVESISFINNPANKGLVLKSLSKELRLSKPEDAQAG
                     YEVMRTLYDRRIYPNEEGLRNVIRLLGNSSEQLRKVKVENIVDDRIVRRLEKEGLF"
     gene            4928..5989
                     /locus_tag="VF182_25370"
     CDS             4928..5989
                     /locus_tag="VF182_25370"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6440474.1"
                     /translation="MAANGDNAGFEVIISSDSHVMEPSEILVERVPKSFRDQAPRFPQ
                     LKLGESFQTHPGGSDPTHRITEMETDGVSAEILYPTYLLPHFAMDDAKLQATCFRAYN
                     DWLIDYCSTSPKRLLGIAAISVYDIDEAVKELERCAKAGVKGSIIWQAPHPDLPFHSE
                     HYNKFWEASQALDLPVHLHILTGHGYHKESVFGNKRTGVEAYRGSVNLKLKEIIDAFF
                     ELIFYGVLERYPKLKFVSVENEIGWMPFMLQQWDYYYRRFRGVNPLPITKDPSEFFKT
                     QIFGTFFRDTAAGHSFEWWGQDNCMWSNDYPHGNSTWPESRKYIDRDLGHLPPDVRKK
                     LVYSNVKNLYDIDIAHPVQ"
     gene            6221..>7727
                     /locus_tag="VF182_25375"
     CDS             6221..>7727
                     /locus_tag="VF182_25375"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6440475.1"
                     /translation="MPARYGSDLVVDLLKVLGIEYVALNPGSSFRGIHDSLVNYESGR
                     TPNPEMILCCHEEIAVAVAHGYAKATGKIMPAIVHNVVGLQHASMAIYNAWCDHTPVM
                     VMGGTGPMNSSKRRPWIDWIHTAQVQGNLVRDFVKWDDQPMGVEAFPSSIMRGYRMAM
                     SDPGGPVYLCFDVTDQEAAIEKEIPLPDPKRFRPAAPLQADYDAIKEAAQLLATAQNP
                     VILADYVGRNNEAVMSLVALADLLSIPVIDFGARLNFPNTHSLNLTGRNRELIANADV
                     IMGLDMTDLYGALVRSDPNTHANVPATKPGCKVINVTLKDIVVRSWTSDFQELAPVDV
                     PIVANTRVFLPALAEEIKKQGKFSKSVVDDRRKAMAGQHEEVHARWQADLKKRWDERP
                     IAPPRLAWEIWEALKKEDWLLVAGAFRGWPSRLWTWDKPGLFLGGYGGGGLGYGPAAS
                     VGASLPYRGTGKICVNLQRDGEMLYTPSALWTAANTGVPLLTVMTNNRVYYN"
BASE COUNT         1722 a         2089 c         2200 g         1716 t
ORIGIN      
        1 cagcgttatt cctgcgcctg taagcgtcgc ccattagcac atctcacaga tggcgcgagc
       61 acaatgctta cgcgtatggc caccatccag ccgagctccg agccacctat ccagattcat
      121 atttcttaat cttccttgca aatcgtgctg ctgcgaggat agaagtaagc acaactacgg
      181 aggaccatac atcggctttt cttggccgcc tcgtcgctgt gcttctttca tgacgctgct
      241 gctgccgact gagctctaag gaaaccgcgc cttgagtaaa cttgtaagtc gcccataatc
      301 aacggttaac gagccatcgc aatgcactgc ctccgttgcc ggcatgaaaa tcctcccggg
      361 gtgaagttct gcggcgaatg cggcgcgcga tttgaggcgc tatgtccttc ctgtaaggct
      421 tcaaatccgc caacaaacaa attctgcggc cagtgtggag cgacgctcgg tcaagtcgcc
      481 gccggatcga gttttacctc gcctaagtct tacacgccca aacatctcgc cgaaaagatt
      541 ctcacatcaa aagatgttct cgaaggcgag cgcaagcagg ttacggtgct cttcgccgat
      601 atgaagggct cgatggaact gctcgcggac cgtgatcctg aggacgcgcg caagcttctt
      661 gatcccattc ttgagcatat gatcgacgcc gtccatcgtt atgaaggaac cgtcaaccaa
      721 gtcatgggcg acggcatcat ggctctgttc ggcgcgccgc tggcccatga ggatcacgcg
      781 gtacgcgctt gttatgcggc gctcaggatg caggagtcgg tgaagcatta cgctgaagga
      841 gtcagacgga ccgagggagt acccatccaa ataagaattg gtttgaactc tggagaggta
      901 gtcgtgcgct ccatcggaag tgatctcaag atggattaca cggcagtggg ccagacgacc
      961 catctggctg cacgcatgga acagatggcg atgccgggat cgatcttgat gactgcccat
     1021 gttctcagtt tggcggaaag ctatgtacag gtgaagcctc tgggtccggt taatgtaaag
     1081 gggatgagcg aatccgtgga cctctatgag gtcacgggag caggaatcgc ccggacgcga
     1141 ctccaggccg cggctgggcg aggtcttact cgatttattg ggcgcgatgt cgagaccgag
     1201 cagcttcgca aagccctgga gcaggcgcgg gaggggcatg gtcaagtcgt tgcagtaatg
     1261 ggcgagccgg gagtcggaaa gtcgcggctg tttttcgagt tcactcgctc ccatcgcgca
     1321 gagggatgtt taatcataga atcgggatct gtctcttacg gcaaggcgac tccgtacctg
     1381 cctgttgccg atctgctaaa agcgtatttc aaaattcatg accgtgacac tcagcgcgat
     1441 gtgcgtgaga aagtcaccgg caagctactg acactggaca agactctcga acctgcgctg
     1501 ccggcattgc tgtcgctttt agatgtcccg atagacgatc cgcaatggca aaacttggat
     1561 ccgctccagc gccgccagag caccttggat gcgataaggg gcttgctttt gcgggagagc
     1621 cacgttcaac ctttgctcct ggtctttgag gatctgcatt ggatcgattc ggagaccgaa
     1681 tcgtttctgg atagcttgat cgatagcctg cccacggcgc cacttcttct cctcgttaac
     1741 taccgcccgg aataccgtca tagctggggc aacaaaacat actatagtca attgcgaatc
     1801 gatcccctgc ctcccgaaag cgcgcagcaa ttgctgttgg ctactctggg tgacgatccg
     1861 agtttgcagc cgctcaagca gcttctgatt gagcgcaccg agggcaatcc gtttttcatc
     1921 gaagagagca tccggactct gattgagaca aaagttctag cgggcgaccg cggcaaatat
     1981 cgtcttagtg gatcgtcggt gatcacgcac gtcccagcga cggtccatgc ggtattggcg
     2041 gcccgcatcg atcggcttgc gctcgaacaa aaacgtctgc ttcaatcggc ggcagtagtc
     2101 ggcaaggatg tgccgtttcc gctactgcaa gggattgccg aattaccgga agacgaactc
     2161 cgttgccatc tgggtcatct tcaagcggcg gagtttcttt atgagacgcg cctgttccct
     2221 gatctggagt acactttcaa gcacgcgttg acccacgaag ttgcctacgg cagtgtctta
     2281 caagatcgtc gccgggcgct gcacgcggga atcgtcgaag ctatcgaacg gctctatcct
     2341 gatcgcttga tcgagcaggt cgagttgctc gcgcaccatg ctttccaagg cgagctgtgg
     2401 gagaaagcgg tcactaacct acggcaagct ggcggtaagg cggccgatcg ttctgcccac
     2461 cctgaagcgg tcagttactt cgagcgagtg ctcgaagcgc tcaagcatct gcccgaaagc
     2521 cgtcaaaaaa tcgagcaagc catcgatgtt cgcatcgaga tgcgtagctc gcttctaccg
     2581 ctcggcgaac agctcaagtt gttcgagcgc atgtgcgagg cggaagtgct cgccgagact
     2641 ttgcatgacc aacaaaggct cgggcaggtc tcagcctacc tgagcggtta ctttattcaa
     2701 gcgggtgatg accctattcg ggccgtagag aaaggacaac gggctctcga aatttcctcc
     2761 tcaactaaaa attttgctct tcaggtacag gccaatcatt tcctcgcctc ggcgtaccat
     2821 tacctcggcg actatgatcg cgcgctcacg catttcagaa ggaacgtcga tacactggcg
     2881 ggcgataaga tctacgagcg attcggctta gcttttcttc cttccgtggg ttcccgctat
     2941 cagttggtga tacttttggc ggagcgcggc caatttgacg aagcagccat tcatgggact
     3001 gaagccgttc gcattgccga gaccgctaat catccgttta gcctcagcac ggcatactat
     3061 gctgttggct atctgtatat cagcaaaggc gcgatcgacc aggcgatcgg cctgctcgaa
     3121 cgcgagctgg aattttgccg gtcatggaac cttcatcaga atcttcggcg tgtgggggct
     3181 gctctcggat atgcctatgc tgcggctgga ctagatctta aggccctgcc tctcctggat
     3241 gttgcatacg agcggttaag gcttgcctcg gcgcgtgcac agttggcgca ggcatatcta
     3301 ttgattggca ggcgagaaca agctgactta atcgccagtc aggcccttga tacttcccga
     3361 agtcaggggg aacgaggccg cgaagcagaa gcactttacg tgctcggtga gattggtttg
     3421 tccgctattc cggtggatat cgcgggagtc caagatcatt atcgccaggc aatcgctcta
     3481 gccgatgaaa tcggtttgcg cccgctcgtc gcgcactgcc atgtgggcct tgggaaattg
     3541 tatcagcgca ccggcgattt acagcgagcg agggagcatc ttacgaatgg cgttgcgatg
     3601 atgcgcgaga tggaaatggg attgtggttg gatagagcac gcgctgagtt ggagaaattg
     3661 ggttaacgct tagaaccgat tagtatccta cttcatggca tttcaggagg agacaatatg
     3721 acacttggtt tcgataagct gcggctgctc tttgttattg tcctgacaat tatatcgccc
     3781 gtttcggcaa ccgccgcgtc cgcgcctcac aaagccatct ttaccttcgg cggtctcaac
     3841 gaacgcagcg gtgtgctgtt cgtcgcccgc gacgcgggcc tatttcacaa acatgggctc
     3901 gatgccactg tagttaatgt tcgcaatgcc gccgttggca tgtcggctct ggcctcgggg
     3961 gaaacacagt ttcacgtggg ttccgccacc ggctcttcga tcggcgccat ggccggcgga
     4021 gtcgatttgg cgttcatcgc cggtctcatc aacaaactcg acggcacatt cgttgtcgcg
     4081 ccgaagattc gcgcgccgga ggatctcaaa ggcaagcgaa tcggcgtgca gagcatcggc
     4141 ggcggtgtct ggatgtttac gatgctggcg ttcgaaaaat ggggcctcaa ccccgagcgg
     4201 gataacattc agtttcgagt gcttggtgat cagtcggtga ttgcccaggc gatgacccaa
     4261 ggaatcgtcg acggcgctta tctgggatac acgtttggcg cgcagatgga acgccagggc
     4321 taccggatat tggccgatct ggcgaaaatc ggcgtgccgt atcaggggct tggcgtcatg
     4381 gcgaggcgca atctcgtcga tcgcacgccg gaagttgccg agaaagccct gcgagcaatg
     4441 gtggagtcca tcagctttat caataatcct gccaataagg gattagtatt aaaaagcttg
     4501 agcaaggaac tgcgcctgtc gaaaccggag gatgcccagg ccggctacga ggtgatgcgc
     4561 acgctctatg accggcgcat ctatcccaat gaggaagggc tgcggaatgt aatccgcttg
     4621 ctgggaaaca gcagcgagca actgaggaag gtaaaagtgg aaaatatcgt cgacgatcgg
     4681 atcgtccgca gacttgaaaa agagggattg ttctaaatcg cagcggcaaa gatggtcttc
     4741 gtgatctgct aaacctctgc gtactctgtg tctctgtgtg taaacgtccg gattattcac
     4801 cgccgaggcg cagagggcgc ggagtcggaa aatttagcaa tcttgacggt tcaaggggcg
     4861 cagtgataca gcttcaaata gacctttaaa tggattacca atcaggtgac acaaaaggag
     4921 gaacgctatg gctgccaatg gtgataatgc gggcttcgaa gtaatcatct cttccgactc
     4981 ccatgtaatg gagccgtctg agattttggt tgagcgggtg ccgaagagtt ttagagacca
     5041 ggcgccgcgt ttccctcagt tgaaactcgg tgagagcttt caaactcatc ccggaggttc
     5101 ggatccgaca caccgaatca ccgagatgga aaccgacggc gtcagcgccg aaatcctgta
     5161 tccgacttat ctcctgccgc acttcgccat ggacgatgcc aagctgcagg ccacctgttt
     5221 ccgcgcctac aacgactggt taattgacta ttgcagcaca tcgccgaagc gtttgctcgg
     5281 catcgccgcc atttcggttt acgacatcga cgaagcggtg aaagagcttg aacgctgcgc
     5341 caaggccggt gtgaagggtt cgatcatctg gcaagcgccg cacccggacc tgccgtttca
     5401 ttccgagcat tacaacaaat tctgggaagc gtctcaggca ttggatctgc cggttcattt
     5461 gcacattctc accgggcacg gctaccacaa ggagtcggtg ttcggcaaca aaagaaccgg
     5521 agtcgaggcc tatcgcggca gcgtcaattt aaagctcaaa gagattatcg acgccttctt
     5581 tgaattgatc ttctacggcg tgctggagcg ttatcccaag ctcaaattcg tcagcgtaga
     5641 aaatgaaatc ggctggatgc ccttcatgct gcagcagtgg gattattatt acaggagatt
     5701 ccgcggtgtg aatcccctgc cgatcaccaa agatccgagc gaatttttca agactcagat
     5761 cttcggcact ttcttccgcg acactgccgc cggtcacagt ttcgaatggt ggggccagga
     5821 caactgcatg tggtcgaacg actatccgca tggcaattca acctggccgg agtcgcgcaa
     5881 gtatatcgac cgcgatctcg gtcatttgcc gccagacgtc cgtaaaaagc tggtttattc
     5941 gaacgttaaa aatctctatg acatcgacat agcccatccg gtgcagtagg agcggcgatt
     6001 tgctgctatg aacgatcggc gcgctggcgg actgaaaacg tccgcggcgc gctttttttt
     6061 cagcaaatac acaatgattt tctccgggta aagaggcaaa gctcgcagat gtagcggtcg
     6121 ggaaattatt tttttgcgcc ttctgtctta gcgcgattaa tcctcgaccg tttcgttttc
     6181 ttgaagaggt aaaaataaaa atctagccat cggaggtttt atgccggcac ggtatggttc
     6241 cgatctggtc gtcgatctac tcaaagttct cggcatcgaa tacgtcgcgc tcaatcccgg
     6301 atcgagcttt cgcggtatcc atgattctct ggtgaattac gaaagcggcc gcacgccgaa
     6361 cccggaaatg attctgtgct gtcacgaaga gattgccgtg gcggtggcgc acggctatgc
     6421 caaggcaacg ggcaagatca tgccggccat cgtccacaac gtcgtcggcc tgcagcacgc
     6481 ttcaatggcg atttacaatg cgtggtgcga ccacacgccg gtaatggtca tgggcggcac
     6541 cgggccgatg aactcgagca agcgacggcc ttggatcgac tggattcaca ccgctcaggt
     6601 gcaaggcaat ctggtgcgcg acttcgtcaa atgggacgac caaccgatgg gagtcgaagc
     6661 ttttccgtcg tcgatcatgc gcggctatcg gatggccatg agcgatcccg gcggtccggt
     6721 ctatctctgc ttcgatgtga ctgatcagga ggccgcgatc gaaaaagaaa tcccgttgcc
     6781 tgatcccaaa cgatttcgtc cagcggcccc cctgcaagcg gactatgacg ccatcaaaga
     6841 agccgcgcaa cttttggcca cggcgcaaaa cccggtgatt cttgccgact atgtcgggcg
     6901 caataacgaa gcggtgatga gcctggtggc gctggccgac ctgctgtcga ttccggtcat
     6961 cgactttggc gcccgattga actttcccaa cacacattct ttaaatctca ccggcaggaa
     7021 tcgtgaactg atcgccaacg ccgatgtgat catgggcctc gacatgaccg acctttacgg
     7081 cgcgctggtg cgctcggatc ccaacacgca tgcgaacgtt cccgcgacca aacccggctg
     7141 caaagtgatc aatgtcacgc tcaaagatat cgtagttcgc agctggacca gcgactttca
     7201 ggaactagcg ccggtggacg tgccgatcgt cgctaatacc agagtctttc tccccgctct
     7261 cgccgaagaa atcaaaaaac aaggaaaatt ttccaagagc gtggtcgatg accggcgcaa
     7321 agcgatggcg gggcaacatg aagaggtgca cgcgcgctgg caggccgact tgaagaagcg
     7381 ttgggacgag cggcccatcg cgccgccgcg tcttgcgtgg gaaatttggg aagcgctgaa
     7441 aaaagaagac tggctactgg tcgccggcgc ttttcgcgga tggccaagcc ggctgtggac
     7501 ctgggacaag cccggactgt ttcttggcgg ctatggcggc ggcggcttgg gatatgggcc
     7561 ggctgcatcc gtcggcgcat ctctgcctta ccgcggcacg ggcaagatct gcgtcaacct
     7621 gcagcgcgac ggcgagatgc tctacacgcc tagcgccctg tggaccgccg ccaacaccgg
     7681 tgtgccgctg ctgacggtaa tgaccaataa ccgggtttac tacaacg
//
LOCUS       DASUVS010000484         7288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1087274, whole genome shotgun sequence.
ACCESSION   DASUVS010000484 DASUVS010000000
VERSION     DASUVS010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087274"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1838)
                     /locus_tag="VF182_25380"
     CDS             complement(60..1838)
                     /locus_tag="VF182_25380"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6440476.1"
                     /translation="MSKAQKIEKPRAEWGSDVVVDLLKAFEFEYIAINPGATFRGLHD
                     SLVNYGGNHAPEIILCTHEEIAVALAHGYARAKGRPMAAAVHNVVGLQHASMAIYNAW
                     ADRLPVIVLGGTGPMDTANRRPWIDWVHTALVQGNLVRDFVKWDDQPASVEAVPDSFI
                     RAYRLATTEPQAPVYICYDGDVQEKQLEGEVSFPSLDRYPAPLPLQAPQEGFDKTIRS
                     LLEAKKPVIITDWVGRKEAGFQALGDLAEILGAPVLDQFGRFNFPVQHPLNLTGQSDK
                     VIPQADLILALDVPDLEGATSRRAQEKGNRRPTPLMQAGAKIINVGLDDLLVRAWATD
                     FNKLREADLMITADTSVFLPELVRRLKSEKTLNDSKDEIGRRRADWGKIYEDKKTALE
                     KELKTKWDEKPISMTRIYAEMIEALKGEDWVLTNGSSQGKENLYLPAAKFNQILGKYK
                     GGGLGYGLPASLGAALALKGSGKFCVNIQPDGDLLFTVSALWTAVHHQIPLLSIVCSN
                     RSYFNDEEHQERVAHQRNRPVENKTIGIRIEDPNVDFGAMARTYGCWGAGPISEPKDL
                     IKTLREAVKVVKEGKPALVDVVCQMR"
     gene            complement(1945..2562)
                     /locus_tag="VF182_25385"
     CDS             complement(1945..2562)
                     /locus_tag="VF182_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6440477.1"
                     /translation="MIQLYTWATPNGKKVSVMLEELELPYEVHPINIAKGDQLRPEYL
                     AINPNNKIPAIIDSDGPGGKPLKLFESGAILMYLADKTDRFLPREMAKRYEVIQWLMF
                     QMGGVGPMFGQANYFYRLEEKVPFAIERYYKEAIRLYKVLEQTLEQRDYLAGEYSIAD
                     IATYPWVGRHDGHNVKLEDFPNVKRWFDAISQRPAVKRGMAIPKI"
     gene            complement(2559..3803)
                     /locus_tag="VF182_25390"
     CDS             complement(2559..3803)
                     /locus_tag="VF182_25390"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6440478.1"
                     /translation="MLSLSALSKKYGLIALAFLHIAVSRGLHGTFGVFFVAMVSAFGW
                     SRATTAGAISLAIVFEGACLPFAGALTDRIGGRKTLMLGGLVLAVGLGFASTISSIWH
                     FYFWVGIVSAFGIALIGMVPHVAILSREFPQRRGTALGIAWAGGGVGIVLLVPLAQLL
                     IDKWGWSVAYIGIAMVTALLVIPPVQLFLPRVSTISPEPPSTTAKSAAKHNETDWTVR
                     RALTNPAFWLLFIARTLASMGNQVIVTHQIAHAVDVGYATVFAASIFGLMGVISIGGR
                     ILFGYLADVMNRQMVFTWVQAISAVGIVALLMLHDATSPWLLYTYAVCYGLGQGSRAL
                     VLSAISADIFHGKHFGAIFGYFTFSIGLGGAIGAWLGGFLFDLNHSYKIAFWVSLACL
                     IISVFIVLASAKVVKAQKGSYS"
     gene            complement(3852..5279)
                     /locus_tag="VF182_25395"
     CDS             complement(3852..5279)
                     /locus_tag="VF182_25395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6440479.1"
                     /translation="MDSRINWLLLINAALGNFMAGTASRIFTVSLPTVANSLETSIIA
                     ISWAVIAYQISQISLSLVFGRVGDIYGRHTLFGVGLVISSLGALMCGLSQNVTQLIVF
                     RFFQGVGASMTQSQGRALAMESVPKDSSGRAQGYMTTAFHSGVLIGPSVGGLIIDYIH
                     WRAVFFFLVPIGLSGILLTLFNRRTGNISTRGTPPSSQRAIDYLGAILLVAATIALIA
                     SIDHRIMEAAGAAMRFVFVSGFVLLFGGFIYREMTCASPILDLSLFRIRMFSLSSLTL
                     LLVGVAQVMIGFMLPFYLQDILRLTPSFIGLLFISAPIFTVTLSPFVGWAVDKVGPRL
                     PATVGISFLVSAALLGSFLRSDSHWSWAVAVLALWGLATALFFTSNHTAMITSVPAEH
                     RGVATGAIYVMFGLGSTFGISLGTLLLTAAFRYYSGDPAATPTPANATVFVQAMNFSF
                     FTGGIMGLVAMVCSSMRGKGRAAQQ"
     gene            complement(5514..5915)
                     /locus_tag="VF182_25400"
     CDS             complement(5514..5915)
                     /locus_tag="VF182_25400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440480.1"
                     /translation="MSFDPQAVFANLAEKERIKGHHSPEGRAIRMLSRALNGYSSGNL
                     SGRDVAVLCDQAIEDWLKAKCKFSPWSMRSLPELLVMAVQARWISRLEAVRLQKLHNT
                     RESINQQRADVPGLEVKSTLKLCIELVEKHW"
     gene            6284..7246
                     /locus_tag="VF182_25405"
     CDS             6284..7246
                     /locus_tag="VF182_25405"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX6440481.1"
                     /translation="MEPLPRGANFQRAKMLWEIGLHIAGDPNTPYYGPRDMCISVGSG
                     SNENFRPWLRMSTGSPILAHAVCHNELEMAMINPSGLLTQAYRGTGLFSQPLPIRIIA
                     VYPSLDHFVYLIHPRTGLKSLAEIKEKKFPLRLSIREDATHSTRVLVDQTLAAYGMTL
                     KDIESWGGSFQLNGGPGDQRRQDAMSAGTVDAVFDEGLPLWFDDALAAGLRPITLEDF
                     TFKYLVDLGWRKYTIRPGRFKNLKEDHTCIDYSGWPLYTRASLPDDDAYKVCEALSAR
                     KDEIYWEDSYTGIGQLGENTDATPRDVPLHPGAERWYREHGFKV"
BASE COUNT         1749 a         2125 c         1886 g         1528 t
ORIGIN      
        1 gtcagagaga acctcaagca agttgattga ggctctctca tgtttgtgcg ctagtaaact
       61 caccgcatct ggcaaaccac atcgaccaat gcaggcttgc cttccttcac taccttaacg
      121 gcttcacgca gcgtcttgat gagatcttta ggctcgctaa tcggtccggc gccccagcac
      181 ccataggtcc gcgccatagc gccgaaatcg acattcggat cttcgatacg gatgccgatg
      241 gttttgttct ccaccggacg attgcgctgg tgcgcaacgc gctcctggtg ttcctcgtcg
      301 ttgaaataag agcgattgct gcaaacgatg ctcaggagcg ggatctgatg atggaccgcg
      361 gtccataatg cgctgacggt gaagaggaga tcgccgtcgg gctggatgtt gacgcagaat
      421 tttcctgagc ccttgagcgc cagcgcggcg cccaacgatg ccggcaagcc gtagcctaac
      481 ccaccgcctt tgtatttgcc caggatctga ttgaacttgg ctgcgggcag gtagaggttt
      541 tcctttcctt gcgaactgcc gttggtcagc acccagtcct cgcctttgag cgcctcgatc
      601 atttcggcgt aaattcgcgt catcgaaatc ggcttctcgt cccattttgt ttttaattct
      661 ttttccagcg cggttttttt atcctcgtag atcttgcccc aatcggcgcg acgccggccg
      721 atctcgtcct tcgaatcgtt cagtgtcttc tccgacttca agcgccgcac caactcgggc
      781 aagaataccg atgtatccgc ggtgatcatc aagtcggcct cgcgcagttt gttgaaatcg
      841 gttgcccagg cgcgcacgag caagtcatcg aggccgacat tgatgatctt ggcgccggcc
      901 tgcatcaacg gcgtcggccg ccgattgccc ttttcctggg cccggcggct cgtcgcgcct
      961 tcgaggtcgg gaacatccaa agccaggatc agatcggcct gcgggatcac cttgtcgctc
     1021 tgcccggtta ggttgagcgg atgctgcacg ggaaaattga atcggccaaa ttgatcgagc
     1081 acaggcgcgc cgagaatctc ggccaaatca ccgagcgcct ggaagccggc ttctttcctg
     1141 ccgacccaat cagtgataat gaccggcttc ttggcctcga gcagcgagcg aatcgtcttg
     1201 tcaaaacctt cctgcggcgc ttgcaacggc aaaggcgcgg gataacgatc cagcgacggg
     1261 aagctcactt cgccttcgag ttgcttctct tgaacgtcgc cgtcatagca gatgtaaacc
     1321 ggcgcctgcg gctcggtcgt cgccaatcga taagcgcgga tgaaagaatc ggggaccgct
     1381 tcgacactgg ccggctgatc gtcccacttg acgaagtcgc ggacgagatt gccttgcacc
     1441 aatgcggtgt gcacccagtc gatccacggc cgccgattgg ctgtatccat cggcccggtg
     1501 ccgcccagca cgatcaccgg cagccggtct gcccacgcgt tgtaaatcgc catcgaggca
     1561 tgctgcaggc cgacgacgtt gtgcaccgcc gcggccatcg gccggccctt ggcgcgcgca
     1621 tagccgtgcg ccagagcgac cgcgatttcc tcatgagtac agagaatgat ctccggcgca
     1681 tgattgccgc cataattgac cagcgaatca tgcaggccgc ggaaggtcgc gcccgggttg
     1741 attgcgatat actcgaactc gaatgctttc aacagatcta cgaccacgtc cgaaccccac
     1801 tctgcacgcg gcttctcgat tttttgtgct ttgctcatat tctgatcctt tcatgccatg
     1861 aggtcgctag ccgaataatc accctgattg cgttcgaacg acctgcacga acacgtttca
     1921 cgaacacgat tcccagacac ggctttatat tttcggtatt gccatccccc gctttaccgc
     1981 cggccgttgc gaaatcgcat cgaaccaacg tttaacgttg ggaaaatctt ccagcttgac
     2041 attatggcca tcgtggcggc ccacccaagg gtaggttgca atgtcggcga ttgagtattc
     2101 gcccgccaga taatctcttt gctccaaggt ttgctcgagt actttgtaca ggcgaatcgc
     2161 ctctttatag taccgctcga tggcgaaggg aaccttttct tccagccgat aaaaataatt
     2221 agcttggcca aacatcggtc ccactccgcc catctgaaac atcagccact gaatcacttc
     2281 gtagcgcttg gccatctccc gcggcaaaaa cctatccgtc ttgtccgcca gatacatcag
     2341 gatcgcaccg gactcgaaga gtttcagcgg cttgccgccg ggtccatcag aatcgatgat
     2401 cgcaggaatt ttattgttgg gattgatcgc cagatattcg ggcctgagtt gatcgccctt
     2461 ggcgatgttg atggggtgaa cttcataggg aagctccaac tcttcgagca tgaccgaaac
     2521 tttttttccg ttgggcgtgg cccaggtata gagttgaatc atgagtaact tcctttctga
     2581 gctttgacga ctttagcgct cgccagaacg ataaacacag aaataattaa acacgcaaga
     2641 gaaacccaga atgcaatctt ataactatga ttgagatcga aaaggaagcc gccaagccat
     2701 gcgccgatgg cgccgccgag cccgatactg aaggtgaagt atccgaagat cgcgccgaag
     2761 tgcttgccat gaaaaatatc cgccgagatc gccgaaagca ccaatgcgcg cgagccctgg
     2821 cccagcccgt agcaaaccgc ataggtgtag agcaaccagg gcgacgtcgc atcgtgcaac
     2881 atcaatagag cgacaattcc aacagcggag attgcctgga cccaggtaaa caccatttgc
     2941 cgattcataa catcggcgag gtaaccgaag agaattctgc cgccgatgct aatcactccc
     3001 atcaaaccga agatcgacgc ggcaaagacc gtagcgtaac cgacgtcgac tgcatgggcg
     3061 atctggtgcg tcacaatcac ctgattaccc atgctcgcca acgttctggc gataaacaga
     3121 agccaaaatg ccggattcgt caaagcgcgc ctgaccgtcc aatccgtttc gttatgcttt
     3181 gctgctgatt tagccgtcgt gctcggcggc tctggactga tcgtggagac gcgcggcaga
     3241 aagagttgca ccggcggaat taccagcaac gccgtgacca tcgctatacc gatataggcg
     3301 acggaccagc cccatttatc gatcagcaat tgcgccaacg ggactaacaa tacgatgccc
     3361 acaccaccac cggcccaagc gatgcccagc gcggtgccgc ggcgttgcgg aaactcacgt
     3421 gacaaaatgg caacgtgagg caccatgccg atcaaggcga tgccgaaggc cgagacaatc
     3481 ccaacccaga aataaaaatg ccagatcgaa gaaatcgtcg aagcaaatcc taatccgacg
     3541 gccagcacca aaccaccgag catcagagtc ttgcggccgc cgatgcgatc cgttaaggcc
     3601 cccgcaaaag gcaaacaggc gccctcgaag acaatcgcca aggagattgc acccgccgtc
     3661 gttgctcgcg accagccgaa tgcgctgacc atcgcaacga agaaaacacc gaaagtgccg
     3721 tgaagaccgc ggctgactgc gatgtgcaaa aacgccagcg cgatcaggcc gtattttttt
     3781 gacagtgctg ataatgatag catcgcctga gttatttcag ctttccgttt tcatctttca
     3841 tctgttctgc atcattgctg cgccgcccgc cccttgcctc tcattgacga acaaaccatt
     3901 gcgactaagc ccataatgcc gccggtgaaa aaactgaaat tcattgcctg cacaaacacc
     3961 gtcgcgtttg ccggcgtcgg cgttgccgcc ggatcaccgc tgtaataacg aaatgccgcg
     4021 gtcaggagca gcgtgcccag ggatataccg aatgtcgatc ccagcccgaa catgacatag
     4081 atcgcgccgg tggctacgcc gcgatgttcc gccggcaccg acgtgatcat tgcggtatga
     4141 ttggatgtga agaacagcgc cgtagccaat ccccacaagg cgagcaccgc aaccgcccac
     4201 gaccagtgcg aatcgcttcg caggaaggaa cccagcaacg cggcagaaac tagaaaagaa
     4261 atgccgacgg tcgccggcaa gcgcggcccc accttatcca ccgcccaacc gacaaacggc
     4321 gacagcgtca cggtaaaaat gggcgcgctg atgaacaaca ggccgataaa cgacggcgtt
     4381 aggcgcagaa tgtcttgcag gtagaacggc aacatgaagc cgatcatcac ttgggcaacg
     4441 ccgaccaaaa gcaaagttaa actgctcaga gaaaacatcc ggatgcgaaa cagcgagaga
     4501 tcgagaatcg gactagcaca ggtcatctcg cgataaataa agccgccgaa gagcaagaca
     4561 aagccgctga caaatacaaa ccgcatcgcc gctccggcag cttccatgat ccgatgatcg
     4621 atgctcgcga tcagcgctat ggtcgccgcg acaaggagga ttgcgccgag ataatctatg
     4681 gcgcgctgcg atgaaggcgg tgtcccgcgc gtcgagatgt ttcctgttcg acgattgaag
     4741 agtgtcaaca gaataccgct gagcccgatg ggaaccagaa aaaagaacac cgcgcgccaa
     4801 tgaatatagt cgatgatcaa gccgccgaca ctgggaccga tgagcacgcc tgagtgaaat
     4861 gccgtggtca tatacccctg cgctcttccc gatgagtcct tgggcacgga ttccatggct
     4921 aatgcgcgtc cctgagattg ggtcatggaa gcgccgacac cttgaaagaa tcgaaacaca
     4981 atcagctggg ttacattctg agacaggccg cacatcaacg ctcccagcga cgaaatcacc
     5041 aagccaacgc caaagagtgt gtgacggccg tagatatcac cgactcggcc aaagaccagc
     5101 gataaactaa tctgcgaaat ctggtaagca atgaccgccc aggagatcgc gatgatgctc
     5161 gtttcaagac tgttggcgac cgtcggcaac gaaacggtga aaatccggct tgccgttccc
     5221 gccatgaaat tgccgagcgc cgcgttgatc agcagtaacc agttgatgcg tgaatccatg
     5281 tctaactagg ttaaggggaa tggatagaat gtattgcggc gagagattct tttgctaagg
     5341 cagcttatcg ttgcagcgtt acaacgtttc ctcgcaaatc aacttaaacc acgaagcgca
     5401 cgaagaccac gaagaattca tacatttatc tttttacttc gtgctcttcg tgtccttcgt
     5461 gctttgatag tcttttcaac ccgattgctc acgaaacaca tcacagcttt cggtcaccag
     5521 tgtttctcga cgagttcgat gcaaagcttc aatgtggact tcacttccag acctggcaca
     5581 tctgcgcgct gctgattaat cgactcccga gtattgtgaa gcttttgtag acgcacggct
     5641 tctaagcggc tgatccagcg cgcctgcacc gccatcacga gcaattcggg caagctgcgc
     5701 atcgaccatg gtgagaattt acacttggcc ttgagccaat cttcgatcgc ctgatcacac
     5761 aaaacggcaa cgtcgcggcc ggagagattc ccggacgaat agccgttcaa ggcgcggctc
     5821 aacatccgga tcgcgcgtcc ttccggcgaa tggtgcccct tgatacgctc tttctccgca
     5881 agattggcaa agacggcttg tggatcgaaa ctcatgatta cgaaagctcc cgctaaatcc
     5941 aatggatccg gaaccgacgg actggacgcc gactatatgc cgattccgtc attcccgaat
     6001 gctcctatcg ggaatccatc ttgctttatt cttcctctcg cacccgcctg gatgcccgcc
     6061 ttcgcgagca tgacggactt tcactacgcg aggcaaagta tcttagtgag tacgatcaaa
     6121 atactttacc acgaacttta taacgaatac gaaagtacag agctgtgatt taaatacagt
     6181 ttcgtgacga gtcattctca ttcatcaaaa acgatgcact gttgacaccg acaagccgaa
     6241 atcactaagg tagcgccgga caatcacttt actggaggta ctaatggaac cccttccgcg
     6301 tggcgcaaat tttcagagag ccaaaatgct ttgggaaatc ggcttgcata tcgccggcga
     6361 tccgaacact ccttattacg gcccgcgtga catgtgcatt tccgtcggca gcggatccaa
     6421 cgaaaacttt cgtccctggt tgcgcatgtc caccggctcg ccgattctgg cgcacgcggt
     6481 ctgtcacaac gaattggaaa tggcgatgat caacccgtcc ggcctgctca cccaagctta
     6541 tcgtggaaca ggcttattct cacagccgct gccgatcaga atcatcgcgg tttatccatc
     6601 cctggaccat ttcgtctatc tcatccatcc gagaactggc ttgaagtcgt tggccgagat
     6661 caaagagaaa aagtttcctc tgcgcctctc gatccgggaa gacgcaacgc actccacacg
     6721 cgtgcttgtc gaccaaactc tggcggcgta cggcatgacc ttgaaggaca tcgaatcctg
     6781 gggcggcagt ttccaactga acggcggccc cggagatcag cgacggcagg atgccatgag
     6841 cgctggaacg gtcgatgcgg ttttcgacga ggggttgccg ctttggttcg acgacgcgct
     6901 cgccgccggt ctgaggccga tcacgttaga ggattttacc tttaaatatc tggtcgatct
     6961 cggctggcgc aaatacacta ttcgccccgg acgattcaag aacctcaaag aggaccacac
     7021 ctgcatcgac tacagcggct ggccgcttta tactcgcgcc tcgctgccgg atgacgacgc
     7081 ctataaagtc tgcgaggcgc tctcggcccg caaggacgag atttattggg aagactctta
     7141 cacgggcatc ggccagctcg gtgaaaacac cgatgccact ccgcgcgacg tcccgctcca
     7201 tcctggcgcc gagaggtggt accgagagca cggctttaag gtgtgacaaa cagcgtgaag
     7261 gagctgatat gaagacgttc ttctacat
//
LOCUS       DASUVS010000485         4775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_434968, whole genome shotgun sequence.
ACCESSION   DASUVS010000485 DASUVS010000000
VERSION     DASUVS010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434968"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..695
                     /locus_tag="VF182_25410"
     CDS             240..695
                     /locus_tag="VF182_25410"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6440482.1"
                     /translation="MKRFKPRTIRLIGRFHAWLWTLTHGRFVDAAKKAPFLLLTTKGR
                     KTGRLRTTPVLYLEDGRDFIVIASFGGNDMHPAWYLNLKEHPEAEVVIRGERRRIIAR
                     EISIDQKKLIWPRLVKLYPQFEIYQQRTSREIPLLRLTLRTREIKTIWP"
     gene            1302..1523
                     /locus_tag="VF182_25415"
     CDS             1302..1523
                     /locus_tag="VF182_25415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440483.1"
                     /translation="MPASEYKKYYLVSRPHFNEKIRVWLPYASVTSAGDGREFYYHQF
                     KELNSTFATEEQALSFGFIVARAWVDEQL"
     gene            3270..4610
                     /locus_tag="VF182_25420"
     CDS             3270..4610
                     /locus_tag="VF182_25420"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6440484.1"
                     /translation="MEENVRRTVIDALEPNEAVDLVKRLVDIPSPEGEELECARFLYD
                     YMRQAGVEAHVQEVEEGRANIIAVVRGAGDGPTLMLNGHLDTSYTGDFWEDYAALGIP
                     GPNHRPAAYEIGDSIYGLGANNMKGGVAAAFSTLIALKRAGVKLRGHVMASGVAGESE
                     KAPVRGAMRDYQGRRYRGSGHGARYLLTHCEPIDYAIVGEPSGLYVVNGQAGYLFVKI
                     VIWGKFAYLTTRGHRGSGISAIEEAAEVVRALSEWGPRYTERHTFDTGMGVVEPAVTL
                     GAIDSGWPFAPSSVPGVCHIYVNLRTTPALSGQQALAELDARLRELATERPNLKYDLE
                     VYLSNEPSLVTPVDSVLVRTAVEVMEGRMGYPTRPFARGEGNASNDTNTFRRHGIPAI
                     KCGPNARTEPNGAEMNKLHGTHVAKEDIAQAARFYLHMACELSGRTRTELNEKR"
     gene            complement(4594..>4775)
                     /locus_tag="VF182_25425"
     CDS             complement(4594..>4775)
                     /locus_tag="VF182_25425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440485.1"
                     /translation="AIQTPVPAAPMEEKPNEILLGSAARTSIIDNDANKKPRSTRLNN
                     GIANLLNLRIITASR"
BASE COUNT         1180 a         1212 c         1275 g         1108 t
ORIGIN      
        1 agagccgggt ttgagactga gtagaacaca gaagccaaca gtaaaaagcc gcaggtgata
       61 tgtttcaaat ggttccttcc tttgatttcc cgaccggagt aaacacagat tatcaggatc
      121 tgtgatcttc tgtattccga atttttcaat aattcaaaag tattgcgcca acacgggccg
      181 gagttcgaat ccatctatgc gcaggttgaa cacgatctag ttctgaagcg caaacacgaa
      241 tgaaacgctt caaacctcga acaattcgac tgatcggacg attccatgca tggttgtgga
      301 cgctaactca cggcaggttt gtcgatgccg ctaaaaaagc gccgtttctt ttgctgacta
      361 ccaagggccg taaaaccggg cggttgcgaa cgacgccggt gctctacctt gaagatggcc
      421 gcgatttcat cgtaatcgct tccttcggcg gcaatgacat gcatcccgcc tggtacctca
      481 atcttaaaga acatccggaa gctgaagtcg tgatcagagg cgagcgccga aggataatcg
      541 cgcgcgagat aagtattgac cagaaaaaac tcatttggcc gcgcttggtg aagttatatc
      601 cgcagttcga gatctatcaa caacgcacga gccgcgagat tccgctgtta aggttgacgc
      661 tccggactcg cgaaatcaaa acaatatggc cttgacagga atcaggcgcg tatttagcga
      721 aaaactggtg agcgccgaat ttgttgccga gagaatcgga gttgccccaa ctatgcggtt
      781 aaaaagttta gtttgaaagt cgaattctac taaccaaata taaaaaaatg acagcaaata
      841 aaacatggac agccctgata agttctgtga tctttcctta tctcgcgaac tggggataga
      901 ccagacaccg cggttgaaca actaatcacc atcgttgtcg ccgcagcttt caaagcagtc
      961 ctcgctactt cccgaacaca cccgttaccg aataaccatg cgccgccttg tgcgctcact
     1021 tccatatgct ctggctgtat gttttgttcc gcgcagtcgt cgtatattag acagtgggtt
     1081 tctgaagcag tctttctgaa gcagtctaag gttatacgaa aagaccatgc gggcgtttcg
     1141 tatataggtg aggcgcgaga agtttcttca tcttgtggac tttgccgaca tatcgggata
     1201 ggttgtattc catctcagca aactaatcgc gtttttgatc tacatctcga caggtcagcc
     1261 agggccggaa cgcctgcaca gtatcggagg agttccgcgc catgcctgca tcagaataca
     1321 aaaaatacta cttagtttcc cgaccacatt tcaacgagaa gattcgcgtc tggcttccat
     1381 atgcaagcgt tacatccgct ggagatggac gcgagtttta ctatcaccag tttaaggaat
     1441 tgaattcgac gtttgccaca gaagaacagg cactgtcctt cggcttcatc gtagctcgcg
     1501 cttgggttga cgagcaactg tgatcccgag aaataggcca tgtagggtcg ggcggatcag
     1561 ctcgcaagcg ctttcttgaa accgctctct acccgatttt ttcccgcttc gaaccccgct
     1621 tgctctgcgt cctcagatcg acggaatagt tggaaagaga cccgtggaaa aacgggctcc
     1681 atggaggttt ccaatatccg gccaggtcac ggatgtattg gatttccata ttccggtatt
     1741 atcgtcgtaa accggcgtag acggcgatag gaagacccct aaattgggtc ccggcctaga
     1801 atggcccggc tgacaaaatt cacttgaaat gctgaaggac cttcaaccta atcttaatag
     1861 atttcataag taaacagaaa agtttgataa cgcttataga cccggcttga caagtttgct
     1921 gcagctgcaa cgctaatctt taggggcatt tctgggatga agaagaaact tcccgaaaca
     1981 gtgaggagac agatcgccta ttcacatgaa ctcttgaaca ggtcaaacac cttactctcc
     2041 aagttggcag atacagtgga acgaagtcga aacttacttg atgaaagcta taggatcata
     2101 gccaaagtta aagttcggtc gctttttaaa gatgatcctg aacgttagtg cgaagctcag
     2161 gagcagaatt caatcctagc gcctaatttt taggcccgtt ggtgagagcc gcacccagag
     2221 cggagcggag caaattcaac agcgatgaat gaacaggttg taaacaccta aggcaggacc
     2281 gatgaggata gctttttatc aatccaagtc tttgagtact caataccagc ggtttctgcc
     2341 tcttcaaaac gagcgaatct cagtagcggg ttcttgagaa actgaattcg gtcgccgctc
     2401 gcctgccagc taacgcaagc gttcactttc cattctccgg ttatatcatc gtaaacagca
     2461 gatgacacga tggtgtgatc tcggtaaaga acataggcca cgggaaatcc ccccgttcgg
     2521 gttcgggcct tgtttggccc ggtgattaag atccagttaa ctggcggaat gggacataca
     2581 ttgtctgctg aggagggaaa tatgtcaacg tcgcaaaatg caggtgcaaa atgcaggtcg
     2641 tgcgacctat ttagtcacat ttagtcacgc agcatagttg aatcctgccg acgacactgc
     2701 cgagagtgga aaccataact gtttctatcg agcagtaaag tcaccccatc atcgaaggtc
     2761 aaaccgcacc ttcgtcactc gggggctgat gagcagcaaa cgctagcgtg tggatctctc
     2821 ctgtcgagct cttttccggt cagctgatag gtaacacgtt ctgggttgta tcccagtata
     2881 tacgtttggc gcatgtgact ctgatgctag tcaatctacc cttacactca agcctatcga
     2941 agcttacaga aaagggattc cccttcatag cgaccgctaa cacctccaac caaaaagaga
     3001 tgttatttct ccattcttta acttacccga gctggcgtaa agcgtcggtc gtaatatatg
     3061 tcaatcgttt taccgcactt ccacctcatg tatcagcgct ccacttgctt gccgcatccg
     3121 gccggtgtag gtgtcgacga gcgcgacttg ggaggctcac tacggttgtt aagtgagacg
     3181 catggggccc taaagcttgg caggtgaaag tcgaacagct actagggttg cagcggtagc
     3241 gctgtaaaca gaatagttca ggagttcaaa tggaagaaaa tgttcgaaga acggtcattg
     3301 acgccctcga gccgaatgag gccgtcgatc tagtcaagcg tctagtggat atccctagtc
     3361 cggaaggcga agagcttgag tgcgcgcgtt ttctctatga ctacatgcgc caggccggcg
     3421 ttgaagcaca cgttcaagaa gtcgaagaag gaagagccaa tatcatcgcg gtcgtgcgcg
     3481 gcgccgggga cggcccgacc ttgatgctca atggtcatct cgatacatct tataccggcg
     3541 acttttggga ggactacgcc gcgcttggca tacccggtcc gaaccatcgc ccggcagctt
     3601 acgagatcgg cgatagcatc tatggcttgg gagccaacaa tatgaagggc ggtgtggccg
     3661 cggcgttttc gacgctgatc gccttgaaac gggcgggggt aaagttgcgc ggccatgtca
     3721 tggccagcgg cgttgccggc gagagcgaga aggcgccggt tcgcggcgcc atgcgcgatt
     3781 atcaaggccg gcgctatcgc ggcagtggcc atggggcgcg ctatcttctg acccattgtg
     3841 agcccatcga ttatgccatc gtcggcgagc catccgggct gtatgtggtc aatgggcagg
     3901 ccggttatct gtttgtcaaa atcgttatct ggggaaagtt cgcgtacctg acgacacgcg
     3961 ggcatcgcgg ctctgggatt agcgccattg aggaagcggc ggaggtcgtg cgggctttga
     4021 gtgaatgggg tccccgctac accgaacgcc acaccttcga caccggcatg ggcgtggtcg
     4081 aaccggctgt gacccttggc gccattgaca gcggctggcc gttcgcgccc agcagcgtgc
     4141 cgggcgtgtg ccacatctat gtgaatctgc gcaccacgcc ggcattgtcc ggccaacaag
     4201 cattggccga actggacgcg cgtttgcgcg agcttgcgac cgagcgcccg aatttgaagt
     4261 acgatctgga ggtctatctt tccaacgagc ccagtctcgt cacgcccgtc gattcggtgc
     4321 tggtccgcac ggcagtcgag gtgatggaag ggcgcatggg ttatccgacg cggcccttcg
     4381 cgcgcggcga gggcaatgca tccaatgaca ccaacacgtt tcgtcgccac ggcattccag
     4441 ccatcaaatg cgggcccaac gcacgaaccg aacccaatgg cgcagagatg aataaactgc
     4501 acggaaccca tgttgcgaaa gaggatatcg cgcaagcggc gcgcttctac ttgcacatgg
     4561 cgtgcgagct tagcggacgt acccggaccg aacttaacga gaagcggtga tgatgcgcag
     4621 atttaggaga ttggcaatgc cattgttaag acgagtgctt ctgggttttt tgttagcgtc
     4681 attgtcgata atcgatgtgc gcgccgcgga accgagcaga atctcgttcg gcttttcttc
     4741 catcggcgcg gctggaaccg gcgtttggat ggcca
//
LOCUS       DASUVS010000486         3205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1309177, whole genome shotgun sequence.
ACCESSION   DASUVS010000486 DASUVS010000000
VERSION     DASUVS010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309177"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VF182_25430"
     CDS             <1..496
                     /locus_tag="VF182_25430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6440486.1"
                     /translation="AYTGADIVAGCTDSSVPIIAGKWLEEGTHVTCVGGKPDEATLNR
                     IDISLRLGNAPHPWGLPEFGLADEYITYAAKPDHNADFQMKRAGKRAHGIIAEDRAVL
                     LEELLTGKKKGRTSDKQISYSERGNIQGAQFFAVAGKVYELAKARGLGREIPTEWLLQ
                     DIRD"
     gene            567..1658
                     /locus_tag="VF182_25435"
     CDS             567..1658
                     /locus_tag="VF182_25435"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6440487.1"
                     /translation="MPLFLNNEEVEQLLTIKTCMDALEVLYRELGEGAAVAASRTDIH
                     SPTSAAHSGEAPMAHYLKSMSGASPHFGTAALRFSSDIVAWRQAGEGMRREKIPALPG
                     NRWLGIVLLFSSASGELLAIMNDGVIQRMRVGGTNGVASRYLARADAQTVGLIGSGWQ
                     AGTQVMAVCEARAIKKIKVYSPTRANREKFAVETAPLVGVEIVPVSSQEEAVRDVDII
                     VTSTNARKPFLGPEALAQGVHLSCMQRDEANDEALARCRPLVVHTNLTENNSTSSQLK
                     KMANPGFEFRDHPMQRGIDWRTLPTLPDLITGRIKGRENAEQITGFVNNIGLGVQFAA
                     VGAKVFEAAKEKGLGREVPLDWFTQDVHP"
     gene            1706..2698
                     /locus_tag="VF182_25440"
     CDS             1706..2698
                     /locus_tag="VF182_25440"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440488.1"
                     /translation="MTKHLRFLFLLALLLATREAQAQQNFLMGYSSFSANQTPIWVAK
                     EEGFFKRFGTEPDLILIEGGTRGAQALISGDIPIMGMAGQPVISARARGADLTMIAGT
                     VNKMNYVLVGAPNMKKPEDLKGKRIGAAQAGTASYHAVLLGLKHWGLEARRDRITILQ
                     IGNQGARVASLQSGGSDAIIVNPGLGPSLKERGNNIIADFTELPIAYPQQTISVRERT
                     LKSDSEFVEKVMKGVVAGNSFSLDAKNKERVKQVIAKYLRLERVAQAEEHYQAALKVM
                     APKPYVDVPSIASMIEFIAESDPLVAKVKPEMVINHSILKKLDDSGFFEQVSKR"
     gene            2896..>3205
                     /locus_tag="VF182_25445"
     CDS             2896..>3205
                     /locus_tag="VF182_25445"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6440489.1"
                     /translation="MYEDAEAYEAMMGRWSRQLAPLFINFVGVREADKVLDVGCGTGS
                     LTTTLARVTGASQIVGIEPSDGFVEYARAQNVDPRVAFEVGDAQKLPYLDASFDRCMA
                     L"
BASE COUNT          746 a          845 c          968 g          646 t
ORIGIN      
        1 cgcctacaca ggcgcggata tcgtcgcggg ttgcaccgac tcgtcggtgc cgatcatcgc
       61 cggcaaatgg ttggaagaag gaacgcatgt cacctgcgtc ggtggcaagc cggatgaagc
      121 cacgttgaac cgcatcgata tctcattgcg gctcggcaat gcgccccatc catgggggct
      181 gccggagttc gggcttgccg acgaatacat cacctacgcg gcgaaacccg atcacaatgc
      241 ggattttcag atgaagcggg cgggtaaaag ggcgcacggg attattgccg aggatcgcgc
      301 tgttctgctg gaggaacttc taaccggaaa gaaaaaggga cggacctcgg acaagcagat
      361 tagctactcg gagcggggca atattcaggg cgcgcagttt tttgccgtcg caggtaaagt
      421 ttatgagctc gcgaaggcgc gaggtttagg gcgggagatt cccacggagt ggctgctaca
      481 ggatatcagg gactagcaca gatgcttgta ttactccggc agggtcaaac gttcagataa
      541 acatccagca ggagcacgag gaagttatgc cgctattttt gaacaatgaa gaagtcgagc
      601 agctcctgac gatcaagacc tgcatggatg cgctggaggt cctttaccgc gagctcggcg
      661 aaggcgcagc cgtggcggcg tcacgcacgg atatccattc accgacttcg gcagcacata
      721 gcggcgaggc gcccatggcg cattatctca agagcatgag cggcgcatcg ccgcattttg
      781 gcaccgcggc cttgagattt tcttcagaca tcgtcgcgtg gcgccaagca ggggagggca
      841 tgcgccggga aaaaataccg gctttgccgg gcaatcgctg gctgggcatc gtcttacttt
      901 tcagctcggc aagcggcgaa cttctagcga ttatgaacga tggtgtcatt caacgcatgc
      961 gggtgggtgg caccaatgga gtcgcttcca gatacctggc gcgtgcggat gcgcaaaccg
     1021 tcggcctgat cggctccggc tggcaggcgg gaacgcaagt gatggccgtt tgcgaagcgc
     1081 gcgcaatcaa gaagatcaaa gtgtatagcc cgacccgagc caatcgagaa aagtttgccg
     1141 tcgaaacggc gccgctggtc ggtgtggaga tcgtgccggt gagttctcaa gaagaggctg
     1201 ttcgcgatgt cgatatcatc gtgacctcga ctaatgcgcg taaaccgttt ctcggacccg
     1261 aggcgctcgc ccaaggcgtg catctcagct gcatgcaacg cgatgaagcc aatgatgagg
     1321 cgcttgcccg ttgccggccg ttggtggtcc acacgaacct gacggaaaac aacagtacct
     1381 cttcgcaact gaagaagatg gcgaaccccg gctttgagtt tcgcgatcat ccgatgcaaa
     1441 gaggcatcga ttggagaacc ctgccgactt tgcccgacct gattaccggg cgcatcaaag
     1501 gacgggagaa cgccgagcag attaccggat tcgtcaacaa catcggtctc ggtgtgcagt
     1561 ttgccgccgt tggcgccaag gtgttcgaag ccgccaagga aaagggtttg gggcgcgaag
     1621 tgccgctcga ttggtttacc caggatgtgc atccatagac gatggtgaaa acgtcaacga
     1681 atacatgata caagcgagag gggtcgtgac gaaacatctt cgatttcttt ttctcctcgc
     1741 attgctgctc gccacccgag aagcgcaggc ccaacagaat ttcttaatgg gctactcttc
     1801 gtttagcgca aatcagactc ctatttgggt cgcgaaagag gaaggtttct tcaagcgttt
     1861 cggcaccgag ccggatctaa tcctcattga aggcggcacg cgcggcgctc aggcgctcat
     1921 ttcaggggac attccgatta tgggaatggc gggtcaaccg gtaatcagcg cccgcgcgcg
     1981 cggcgccgat ctgacgatga ttgcgggcac ggtcaacaaa atgaattacg ttctggtcgg
     2041 cgcgcccaac atgaaaaagc cggaggatct caagggcaaa cgcatcggcg cggcccaggc
     2101 tggaaccgct tcctatcacg ccgtcttgct ggggcttaaa cactggggac tcgaggcgcg
     2161 gcgggatcga attacgatct tgcaaattgg caatcaaggc gcgcgggtcg cgtcgctgca
     2221 gtcgggaggc agcgatgcga tcatcgtcaa ccccggcctt ggcccatcgt tgaaggaacg
     2281 cggcaataac attatcgccg attttaccga gctgccgatt gcctatccgc aacaaaccat
     2341 cagcgtgcgg gagcgcacgt tgaagagtga tagtgagttc gtcgagaaag taatgaaagg
     2401 tgtcgttgcc ggcaacagct tcagcttgga tgcaaaaaat aaagagcgtg ttaagcaggt
     2461 catcgccaag tacctacgtc ttgagcgagt ggcacaagct gaggaacact atcaggcagc
     2521 gctcaaggtg atggcgccaa aaccgtacgt cgacgtcccc agcatcgctt cgatgatcga
     2581 attcatcgcc gaatccgatc cgttggttgc caaagtcaaa ccggaaatgg tcatcaatca
     2641 ttccatcctc aaaaaactcg acgacagcgg ttttttcgag caggtgtcga aaaggtgagc
     2701 gcgactgctt cgcgtgtcgt tgctctcggg ccggtgaatt acatgcatga gagcgtcaac
     2761 accagctgat tgcttatgag accggagggc gagccgaaat agcccgccct cgaccccgca
     2821 atgacgggtc ggcttcatgc cgtggcctct ggcggctcac tcattagtta ggcatattgc
     2881 cgaaagtagg aatttatgta cgaagacgcc gaagcatacg aagcgatgat ggggcgatgg
     2941 agccgccagc tagcgccatt gtttataaat ttcgtggggg ttcgggaagc tgacaaggtg
     3001 cttgacgtcg gttgtggcac cggctcatta accacaacat tggcgagagt cacaggagcc
     3061 tcgcaaattg tcggcatcga accttcggat ggctttgtgg aatatgcccg agcgcaaaat
     3121 gttgatcccc gggtggcctt cgaggtcggc gacgcgcaaa agcttcctta cctggatgca
     3181 tctttcgacc gatgcatggc gctac
//
LOCUS       DASUVS010000487        11868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1309315, whole genome shotgun sequence.
ACCESSION   DASUVS010000487 DASUVS010000000
VERSION     DASUVS010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11868
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309315"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VF182_25450"
     CDS             <1..914
                     /locus_tag="VF182_25450"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440490.1"
                     /translation="QELKPIRVSLSLGSAQLPLWAAREAGLFSKQGLNAELLGLQSAA
                     RQVQLILAGDTLAASLSGTTPIRARIEGADTMIMMGLLNSFTLSVVTVPEIKNAADLK
                     GKFIGVGSLGGSPTLLTQRLLKKWGLESDVKFLATGGYIESVAALEKRRVHAAVLDPP
                     RAYIGRKKFGFNELANLGQEFKYATTVIVARESQLRKERETFSQFAKAVIQGIHRVKT
                     DREFAIKILGKMLRSSDREILDETYRVFAALYEKVPYPAPEGIQPILDEISLQYPKAK
                     NYKPDDFIDTSIIRQLEGSGFVESVYR"
     gene            complement(991..2523)
                     /locus_tag="VF182_25455"
     CDS             complement(991..2523)
                     /locus_tag="VF182_25455"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440491.1"
                     /translation="MNSKLEGTSRRYFLKQGALGGLAAIFGDSLLKVSLAASKERITL
                     LSSVGLDTLHPYAYTSGPQYGIWLHMIEPLVEVDYAKKQYRGVLAESWEFQGQKWIFR
                     LRKDVRFHDGTPFTAKDVIHSINRIKNDKQSLQKENFRDLTEMQAPDDHTVVFTTEIP
                     NAVFLDRLDNRFMISKAAADKHGDQADQHPLGTGPYKFVSWQRDGNLVMTRNDQYWRA
                     PAAIKEVVLKRVKEDAGRVAGLLSGQGDVINNVPVEELSRFANNHKVRSEKAEGVRMY
                     FLAMNLTHKPFDNKQVRQAFNYAVDPSTIIKHIYEGNGYVMNGPLGSNVIGFDPKIKR
                     YPYDPKKAKELLAGAGFANGLDVKLYFSPDRYPKAREVCQVIADQLAKAGVKAELVSQ
                     EFVIFWGKEGVNGGKLPFYYVGRPAIDADTVYDQYYRSGVSKRVEYKNPEFDKLIDEE
                     QKTGDQKKRVELLQQAGRILMEDAPLVPLYTLAEIYGVARNVVWKARPDERVLSVEMK
                     IK"
     gene            complement(2574..4106)
                     /locus_tag="VF182_25460"
     CDS             complement(2574..4106)
                     /locus_tag="VF182_25460"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440492.1"
                     /translation="MSSNIKGIKRRDFLKGGALAGLSAALGGSLIRVSYAASRDRVTA
                     LSSIGLDSLHPYAHSSSPQYGIWHNMIEPLVEVDYDKKDYYGVLAESWEFQGQKWVFR
                     LRKGVRFHDGTPFGSKDVIHSINRMKNDKLSLQKENFRDVTEMQAPDDLTVVFTTEKP
                     NAVFLDRLQNRFIISKAVADAKGEQADQTPIGTGPYKFVSWQRDGNLVMTRNDNYWRT
                     KVAVKEIVLRRVKEDSGRVAGLLSGQGDVINNVPVEELSRFENNPRVRAEKVEGVRMY
                     FLAMNVTHKPFDNKQVRQAFNYAIDPRAIIKHIYEGNGYLMNGPLGSNVIGFDPKMKR
                     YPYDPKKAKELLSAAGHPDGLEVKLYFSPDRYPKAREVCQVIADQLAKAGVNAELVSQ
                     EFVIFWGKDGVNGGKLPFYYVGRPAADADTVYDQYFRSGVSPRIQYKNPEFDKLIDEE
                     QRTGDPKKRVALLHQAGRILMEDAPLVPLYTLAEIYGAARNVVWKARPDEKILYAEMK
                     IK"
     gene            complement(4160..5692)
                     /locus_tag="VF182_25465"
     CDS             complement(4160..5692)
                     /locus_tag="VF182_25465"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440493.1"
                     /translation="MRWNEPGIKRRTFLKQSAVAGLGVLAGTGWPRLVLAASRERLMI
                     LSSIGLDALHPYAHSSGPQYGIWQHMIEPLVEINYARKDYYGVLAESWKFEGNSWVFQ
                     LRKGVRFHDGSPFSAHDVVHSVNRIKNDKRSLQKSNFDDLTEIRALDDYTVVFTTAQP
                     NAVLLDRLQNRFIVSKTAAEKYGEEVDQHAIGTGPYKFVSWQRDGNLVMTRNDEYWGT
                     KAAIKEIVLKKVGEDAGRMAGLLAGQGDVVNNVPVEEIARLDKHPRVRAEKVEGLRMY
                     FLAMNVTHKPFDNKLVRQAFNYAVDPTAVIKHIYEGNGYVMSGPLGANVIGYDPKIKR
                     YPYDPKRAKELLTQAGLADGLEVRLHFSPDRYPKAKEVCQVIADQLAKAGVKTELVSQ
                     EFAIFWGSDGVNGGKIPFYYVGRPAIDADTVYDQYYRSGRTKRVGFQNAEVDRLIDEQ
                     HKTGDQKKRIALLQQIGRVLMEEAALVPLYTLAEIYGVARNVIWKAHPEEKVLAFDMK
                     IR"
     gene            complement(5761..7299)
                     /locus_tag="VF182_25470"
     CDS             complement(5761..7299)
                     /locus_tag="VF182_25470"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6440494.1"
                     /translation="MARKKTDISRRNFLKKSAALGVGAAVWPGLLRRASAASRDRVVI
                     YHSSVADSIHPYNHSSSPIYGNWQHVIEPLVELDYDKKEYVGVLAESWDFQGKQWVFK
                     LRQGVKFHNGAPLTSKDVAFSIEKMRDEKGGSLQAPNFKDVTEVQTPDDQTVVFVTKQ
                     PLAIFLDRLENRFILSKVAGDKFGDQLYQNPIGTGPYKFVSYQRGGNMVFTRNDDYWG
                     GKAAIKEVVFRKVTEEAARLAALESGQADFINNVPVHEVARFQKHPRVRIDQLEGLRM
                     FLLAMNVAHKPFDNKLVRQAVNYSVDAPAIVKNIFDGIGYPLNGPVGSNVIGADPKHK
                     RYPYDPKKARELLAQAGFKDGCDVQLYYSPGRYPKDREVCQVVAAQMVKGGFRVELIS
                     QEWALFWDKTGVNGGKLPFYYIGRGSLTDADTLYDQYFRTGTTKRTNYSNPELDKIII
                     EQQRTADPKKRVALLQQAGTIIMEEAPMVPLYNLADIYGSARNLIWKKRPDEKVLAWD
                     MKIK"
     gene            complement(7325..8164)
                     /locus_tag="VF182_25475"
     CDS             complement(7325..8164)
                     /locus_tag="VF182_25475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009297088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6440495.1"
                     /translation="MIGLKYLKIWVGGIILAALFVCGVASPLLAPHDPQAQTLEDRLR
                     PPRWAENGSPAYLLGTDNLGRDILSRIIYGSRISLLVGAATVIFAGLLGCTLGAVAGY
                     FGKVADDIVSKISEIFLAFPFLLIAIAIMAFLGQGVVNLIMALMLSRWVQYCRVVRGE
                     VLSIKERDFVTAIKALGGRDGYVLLRHVVPNTIASITVIATFAMAIVIISEASLSFLG
                     LGVPAHIPTWGSMLSEGRSYINRAPWLTIFPGLAIFITVFGINLLGDGLRDIFDPKLR
                     RQR"
     gene            complement(8164..9162)
                     /locus_tag="VF182_25480"
     CDS             complement(8164..9162)
                     /locus_tag="VF182_25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6440496.1"
                     /translation="MNTLSRRILGAIPVLLGISFLVFMLMHIAPGDPVSLLLGDDATP
                     ADIEQTRRELGLDKPLMVQYWDFLTRALTGDFGRSLKFNEPVMKLVFERLPATLELAV
                     ISLIVAIIIAVPLGVYSAIKHNSLFDHAGMSVALIGVSLPNFWLGIMLIYFLGGQLNL
                     LPVAGRIEYGIEVKPITHLYLVDSLITGNLTAFWSALQHLLMPAFTLGTGLAAIVTRI
                     SRSSVLEVMRQDFITTARAKGLSEKTVIWRHILRNAMITIVTILGLQLGALLNGSVIT
                     ETVFSYPGIGELLIQSISARDYKLTQVLILFFAVIYFVVNLLTDFLYSWIDPRIKF"
     gene            complement(9212..10498)
                     /locus_tag="VF182_25485"
     CDS             complement(9212..10498)
                     /locus_tag="VF182_25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX6440497.1"
                     /translation="MNKQEILDKQKKYLFSCVATYYDQPLVVDHAKDHYVYDADGKEF
                     LDFFGGILTVSVGHCNERVTKAIDEQSHKLQHMSTLYANEPQVQLAEKLAQITPGRLE
                     KSFFTNSGTEANETAVLLAQIHTKCQDVIALRHSYSGRSYLALSLSAQSSWRLTPNVV
                     PGVHHIPNAYCYRCPFGLTYPSCNIKCATDIEEAIQTMTSQGRIAAFLAEPIQGVGGF
                     ITPPKEYFKEIVGIVKKYGGLFICDEVQTGWGRTGGKMFGIEHWGVEPDIMTFAKGMA
                     NGVPIGATIATPEVADSMQGNTISTFGGNPVTCTAAHATISVIEEDNLVENARVMGNR
                     LREGLNALKDKYPIIGDVRGMGLMQGMELVGEKKKPDAESTKRLMELTKNHGLLLGKG
                     GTYGNVVRVSPALNVNKDQIDHALKVLDQSFSHLGL"
     gene            complement(10713..11567)
                     /locus_tag="VF182_25490"
     CDS             complement(10713..11567)
                     /locus_tag="VF182_25490"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6440498.1"
                     /translation="MDEIKPVRSGKVNVDGVNLYYELYGQGDPLVLVAGTGISLAPWR
                     IAQVPEFSKHYQVLIYDHRGLGRSDKPDVHYSTRLFAKDCAGLMDALGIKKAHVMGHS
                     MGGRVAQWLALDYPEKIRSLVLSGSGSGKYSDLLEDYPRGVPLDAALEMIEKGYEKYQ
                     HDHWGPGFMFSEQFMKEHPDVVQKYQELIVDEVPPLKCYLRHVVARQCHETTDIVDRI
                     KAPTLVIVGSKDTQEGGTGSHVDSAKALADKIAGSEFVLVEGGRHGYLREMPEKGHPP
                     IIDFLRRH"
BASE COUNT         2623 a         3359 c         3086 g         2800 t
ORIGIN      
        1 ctcaagaact taaaccgatc cgtgtctctc tgagtctcgg ctccgctcag ctacctctgt
       61 gggccgcacg cgaggcgggg ctgttctcga agcagggact taacgccgag cttctcggcc
      121 tgcaatcggc agcccggcaa gtgcaactga tcttggccgg agatacgctg gcggcaagct
      181 tatccggcac cactccaatt cgagcgcgca tcgagggagc cgataccatg atcatgatgg
      241 gactcctcaa ctcgttcacg ctttctgtcg tcaccgtacc ggaaatcaag aatgccgcgg
      301 atctcaaggg caaatttatc ggcgtcggca gcttgggagg ctcgccaacg ctcttgacgc
      361 aacgcctgtt aaaaaagtgg ggcttggaat cggacgtcaa attcctggca acgggcggct
      421 atatcgaaag cgtcgctgcg ctggaaaaac ggcgcgttca cgccgccgtg ctcgatccgc
      481 cgcgggctta catcggtaga aagaagtttg gttttaacga actggccaat ctaggtcagg
      541 agttcaaata cgccaccacc gttatcgttg ctcgcgaaag ccaattacgg aaggagcgcg
      601 agaccttttc gcaattcgca aaggcagtga tccagggaat tcaccgggta aaaaccgacc
      661 gggaatttgc catcaagatt ttaggaaaga tgctcagatc gagcgatcga gaaattctcg
      721 acgagaccta ccgagtcttt gcggcgcttt acgaaaaggt tccttatccc gcccccgaag
      781 ggattcagcc gatcttggat gaaatctctt tacagtatcc gaaagccaag aattacaagc
      841 ccgacgactt catcgatacg agtatcatca ggcaacttga agggagcggt tttgtcgaaa
      901 gcgtctaccg ctaagatttt cacgttgctg cattcgtcgt catctgtggc acgcgataac
      961 actggagaag ccaatcgcta cccggcatgg tcatttgatt ttcatctcga cactgagaac
     1021 tctttcatcc ggcctcgcct tccagacgac gttgcgggcg acgccgtaga tttcagctag
     1081 tgtatacagg ggcaccaacg gcgcatcctc catcaagatg cgtcccgctt gttgcaacaa
     1141 ttcgacacgc tttttctgat cgccggtctt ttgctcttcg tcgatcaact tgtcgaattc
     1201 cggatttttg tattctacgc gtttgctgac tccggaacgg tagtactgat cgtaaaccgt
     1261 atcggcatcg atggccggcc ggcccacgta ataaaagggc agcttgccgc cgttgactcc
     1321 ttctttgccc cagaagatga cgaattcttg agaaacaagc tcggccttga cgcctgcctt
     1381 tgcgagttga tcggcgatca cctggcatac ttcgcgtgct ttggggtaac ggtcaggcga
     1441 aaaataaagt ttgacgtcca agccattggc aaaaccggcg ccggcgagaa gctccttggc
     1501 cttctttgga tcgtacggat aacgctttat tttgggatcg aaaccgatca cgttggagcc
     1561 aagcggccca ttcatcacgt agccgttgcc ctcgtagatg tgcttgatga tcgtcgatgg
     1621 atcgactgca tagttaaacg cctggcgcac ctgcttattg tcgaagggct tgtgcgtgag
     1681 gttcatggcg agaaaataca ttcttacgcc ttcggccttt tcagagcgca ccttatgatt
     1741 gttggcgaaa cgcgaaagct cctccaccgg cacgttgttg atgacatcgc cctggccgga
     1801 caataacccg gcgacccggc cggcatcctc tttaactcgc tttaagacga cttccttgat
     1861 cgccgccggc gcgcgccagt attggtcatt gcgtgtcatc accagattgc cgtcgcgctg
     1921 ccaactgacg aacttgtacg gcccggtacc cagcggatgc tgatccgcct ggtcgccatg
     1981 cttatccgcc gccgctttgc tgatcatgaa gcgattgtcc aaacgatcga gaaaaaccgc
     2041 gttcggtatc tcggtggtaa acaccaccgt atgatcatcc ggcgcctgca tctcggtcag
     2101 gtctctaaaa ttctcttttt gcaggctctg cttatcgttt ttgattcggt tgatcgaatg
     2161 gatgacatcc ttggctgtaa atggtgtgcc gtcgtgaaaa cgcacgtcct tgcgcaatcg
     2221 gaaaatccat ttctggccct gaaattccca tgattcggcg agcacgccgc gatattgttt
     2281 cttggcgtaa tcgacttcga cgagaggctc gatcatatgc agccaaatgc cgtactgcgg
     2341 tcccgatgtg taggcgtagg gatgaagtgt atccaggccg acgctgctca agagggttat
     2401 tcgctctttg ctggcggcca aactcacctt cagcagagaa tctccgaaga tggcagctag
     2461 ccccccgaga gctccctgct tcaggaagta tctccgactg gttccttcta attttgaatt
     2521 cattaccacc tcttcggata acaccggccg cgctgccgga acgattcctc gaatcacttg
     2581 attttcatct cggcgtaaag aatcttttcg tccggtcgcg ctttccacac gacgttgcgc
     2641 gctgcaccat agatttccgc caaggtgtag agcggcacca atggcgcatc ctccatcaag
     2701 atacggcccg cctgatgtaa cagcgcgacc cgctttttcg gatcgccggt cctctgctcc
     2761 tcatcgatga gcttgtcgaa ttcggggttc ttatactgga tccgcgggct gacgccggaa
     2821 cgaaaatact gatcgtagac ggtatccgca tccgcggcag gtcgtccgac gtagtaaaac
     2881 ggcagcttgc ctccgttgac cccgtccttg ccccagaaaa taacgaactc ctgtgacacc
     2941 agctcggcat tgacgccggc tttcgcaagc tgatcggcta tgacctggca tacttcccgc
     3001 gctttgggat aacggtcggg tgaaaaatag agcttcacct ccaaaccatc tgggtggccg
     3061 gccgccgata aaagttcctt ggccttcttg ggatcgtaag gatagcgctt catcttggga
     3121 tcaaagccga tcacattgga gccgagaggt ccgttcatca aatagccgtt gccctcatag
     3181 atgtgtttga tgatcgctct gggatcgata gcgtagttga aggcttgccg cacctgtttg
     3241 ttgtcgaacg gtttgtgcgt cacattcatc gcgagaaaat acattctgac gccttcgacc
     3301 ttctcggccc gtactcgagg attgttttca aatcgggaga gctcttccac cggaacattg
     3361 ttgatgacgt cgccctgccc ggagagcagg ccggcgaccc gtccagagtc ttctttcact
     3421 ctgcgcaaga cgatctcttt gaccgcgact ttggtgcgcc agtaattatc gtttcgggtc
     3481 ataaccagat tgccgtcgcg ttgccagctg acgaacttgt aaggtccggt gccgatcggc
     3541 gtttgatcgg cttgttctcc tttggcatcg gccaccgctt tgctgattat gaagcgattc
     3601 tgcaatcggt ccaggaagac ggcattgggt ttttcggttg tgaaaaccac cgtaagatcg
     3661 tcgggtgcct gcatctcggt gacatcgcga aaattttcct tctgcaaact cagcttgtcg
     3721 tttttcatcc ggttgatcga atggatcaca tctttggagc cgaacggagt gccgtcgtga
     3781 aaacgcacgc ccttgcgcag gcggaaaacc catttctgcc cctggaactc ccatgactcg
     3841 gcaaggaccc cgtagtaatc ttttttgtcg taatccactt ccaccagcgg ctcgatcata
     3901 ttgtgccaaa tgccgtactg aggactcgag ctgtgagcgt acggatgcag cgaatccaag
     3961 ccaatgctgc tgagtgccgt cacccgatct ctcgaagcgg cataactcac cctgatcaac
     4021 gaaccgccca gtgccgcgct caagccggcc aacgcccctc ctttgagaaa atccctgcgc
     4081 ttaatgcctt taatgtttga actcattcgt cctccagtta gcaattgtat tgttttaccc
     4141 gcgttgcgtg attattttct cagcgaattt tcatatcgaa ggccaacact ttctcttccg
     4201 gatgcgcctt ccaaatcacg ttgcgcgcca caccgtatat ttcggccaac gtgtaaagcg
     4261 gcaccaacgc cgcctcttcc atcaggacgc ggcctatttg ctgcagcaac gcgatccgct
     4321 ttttttgatc gcccgtcttg tgctgctcgt cgatcaaccg gtcgacctcg gcgttttgaa
     4381 aaccgacccg cttggttctt cccgatcggt aatattgatc gtagaccgta tccgcatcga
     4441 tcgccggccg gcccacgtag taaaaaggaa ttttgccgcc gttgactccg tccgaccccc
     4501 agaagatggc aaattcttga gagacgagct cggtcttgac acctgccttg gctaattgat
     4561 cggcgataac ctggcaaact tctttcgcct taggataacg gtccggcgaa aaatggagtc
     4621 taacctccaa accatcggcg aggcctgctt gcgtgagtag ctccttggcc cgtttcggat
     4681 cgtagggata gcgcttgatc ttgggatcgt aaccgatgac gttagcgccg agcggtccgc
     4741 tcattacgta accattgcct tcgtagatgt gtttaatgac tgcagtcggg tcgacagcat
     4801 agttgaacgc ttggcgcacg agcttgttgt cgaagggttt gtgcgtgacg ttcatagcca
     4861 agaaatacat tctgagcccc tcgacttttt cggcgcgcac ccgcggatgt ttgtccaagc
     4921 gagcgatctc ttccaccgga acattgttga cgacatcacc ttgtccggcg agcagtcccg
     4981 ccattctccc ggcatcctcg ccaactttct ttaggacgat ttctttaatc gctgccttag
     5041 tgccccagta ctcatcgttg cgcgtcatga ccagattgcc gtcgcgttgc caactgacga
     5101 atttataagg ccccgtgccg atggcatgtt gatcgacttc ctcgccatat ttctccgcgg
     5161 cggtcttact gacaatgaag cgattctgta accgatccag aagaaccgcg ttgggctgcg
     5221 ccgtggtaaa cacgacggtg taatcgtcga gcgctcgtat ttccgtcaag tcgtcgaagt
     5281 tcgacttttg caagctcctc ttgtcatttt tgatgcgatt gaccgagtgg accacgtcgt
     5341 gggcgctaaa cggcgagccg tcgtgaaaac gaactccttt cctgagctga aacacccaac
     5401 tgttgccttc gaatttccat gactcggcga gcaccccgta ataatccttc cgcgcgtagt
     5461 tgatctccac cagcggctcg atcatgtgct gccagatgcc atactgggga ccggagctgt
     5521 gcgcataagg gtgaagggcg tccaagccga tgctgctcag aatcatcagc cgttctcttg
     5581 aagctgccaa aacgagcctc ggccagccgg tgcctgcaag aacgcctaga ccggcgacgg
     5641 cactttgctt gagaaacgtt cttcgtttga tcccaggttc gttccaacgc atcgcgcctc
     5701 ccgtttggcg cactagcaga ccggccaaga gccgctcaga gtttgaaata tgggacaggt
     5761 ttatttgatc ttcatgtccc aagcgagaac cttttcatcc ggccgctttt tccaaatcag
     5821 gtttctggcg ctgccataga tatcggccag attataaagc ggcaccatgg gcgcctcttc
     5881 catgatgatc gtccccgctt gctgcaacag cgcgacacgt tttttcggat ccgccgtcct
     5941 ttgctgttcg atgatgatct tgtccagttc cggattgctg taattcgtgc gcttcgtcgt
     6001 gcccgttcgg aagtactgat cgtagagcgt atccgcgtcc gtgagcgaac cgcggccgat
     6061 gtagtagaac ggcaacttgc cgccgttgac ccccgtcttg tcccagaaaa gcgcccactc
     6121 ttgcgagatc aactcgactc tgaaaccgcc cttcaccatc tgcgccgcaa ccacctgaca
     6181 aacttcgcgg tctttgggat aacgcccggg cgagtaatag agctgcacat cgcaaccatc
     6241 tttaaatccc gcttgagcaa gaagttctct cgctttttta ggatcgtacg gatagcgctt
     6301 gtgcttcgga tcggcgccga taacgttgga tccgaccggt ccattgagcg ggtagccgat
     6361 accgtcgaaa atattcttga cgattgccgg cgcatcgacg gaatagttga cggcctggcg
     6421 gaccagtttg ttatcaaacg gcttatgcgc aacattcatc gccagcaaga acattcttaa
     6481 cccttcgagc tggtcgattc tcacgcgcgg gtgtttttga aatcgggcaa cctcatgaac
     6541 cggcacgttg ttaatgaagt cggcctgacc ggactccaat gctgctaagc gcgccgcttc
     6601 ttccgtcact ttgcgaaaca cgacttcttt gatcgctgct ttgccgcccc aataatcgtc
     6661 gttgcgggta aacaccatgt tgccgccgcg ctggtagctg acgaatttgt agggaccggt
     6721 gccgatcggg ttttgataca gctgatcgcc gaacttgtct ccggcgactt tgctaagaat
     6781 gaaccggttc tccaagcgat ccaagaaaat cgccagaggt tgtttggtca cgaacacgac
     6841 ggtttggtcg tccggcgtct gcacctcggt gacatccttg aagttgggcg cctgcaagct
     6901 gccgcccttc tcatcgcgca tcttctcaat cgagaaagcc acatccttgg aggtcagtgg
     6961 agcgccgttg tgaaatttga cgccttggcg cagtttgaac acccactgct tgccctggaa
     7021 gtcccaggac tcggcgagaa cgccgacgta ctctttctta tcgtagtcca gctccaccag
     7081 cggctcgatg acatgctgcc agttaccgta aatcgggctg gagctatgat tataaggatg
     7141 aatcgagtcg gccacgctgc tgtgatagat cacaacccga tcccgtgaag ccgccgatgc
     7201 gcggcgcaac aatcccggcc acaccgctgc cccgacaccc aaggcggcgc ttttctttaa
     7261 aaagtttcgg cgactgatat cggttttttt tctagccatc gatcctccta aatcgggact
     7321 gttgttatct ctgcctgcgc aatttcgggt cgaagatatc cctcaaaccg tctcctagaa
     7381 gattgatgcc gaacaccgtg ataaaaatcg ccagcccagg gaaaatcgtc agccagggag
     7441 cgcgattgat ataactcctg ccctcgctca gcatcgagcc ccaggtcggg atatgggcgg
     7501 gaacgccgag gccgagaaag ctcaaactcg cttcggatat aataacgatc gccatcgcga
     7561 atgttgcgat caccgtgatg ctggcgatcg tattcggcac cacgtggcgc aacagaacat
     7621 aaccgtcccg cccccccaaa gccttaatcg ccgtgacaaa atcgcgttcc ttgatagaca
     7681 ggacctcgcc gcggacgacg cggcagtatt ggacccagcg gctcagcatg agcgccataa
     7741 tgagattgac tacgccttgc cctagaaacg ccataatggc tatggcgatc agcagaaatg
     7801 gaaaggcaag aaagatctcc gagatcttac tgacgatatc gtcggcaact tttccgaaat
     7861 agccggcgac tgctcccaac gtgcaaccga gcagacccgc aaagatgacg gtggcggcgc
     7921 cgaccagtaa agagatccgg gatccataaa tgatccggct caggatatcc cggccgaggt
     7981 tatctgtccc cagcagatag gccggcgaac cattctccgc ccagcgcggc ggccgcaatc
     8041 gatcctctag ggtttgcgcc tgtggatcat gcggcgccaa cagaggcgag gcgacaccgc
     8101 aaacgaaaag agccgccaaa ataattccgc ccacccagat cttcaaatac tttaatccga
     8161 tcatcagaat ttgatcctcg gatcgatcca gctatagagg aaatcggtca gtaaattcac
     8221 cacgaagtaa ataaccgcga agaacaaaat cagcacctgc gtcaacttgt aatctcgcgc
     8281 tgagatcgat tgaatcaata actcgccaat gcccggatat gagaaaaccg tctcggtgat
     8341 caccgaacca tttagcagcg ctcctagctg gagtccgaga atggtgacaa tggtgatcat
     8401 cgcattgcgc aagatgtgcc gccagatcac ggtcttttca ctcaaccctt ttgccctcgc
     8461 cgtggtaata aaatcctgcc gcatcacttc gaggacgctg gaacgcgaga tacgggtaac
     8521 aatcgcggcc aagcccgtgc ccagcgtaaa agccggcatc agcaagtgct ggagcgcgct
     8581 ccaaaacgcg gtcaaatttc cggtgatcag gctgtccact agatagaggt gggtaatcgg
     8641 tttaacctca atgccatact cgatccggcc ggcgacgggc aggagattga gttgtcctcc
     8701 caggaaatag atcagcataa ttcccagcca gaaattcggc aaagaaacgc cgatgagcgc
     8761 gacgctcatg ccggcatgat cgaagagcga gttatgcttg atagcggaat agacgccgag
     8821 gggtaccgca atgatgatgg cgacgatcaa actgataact gcaagctcta gtgtggcggg
     8881 cagccgctca aaaaccaact tcatgacagg ttcgttaaac ttgagggacc tgccaaagtc
     8941 ccctgtgagc gcgcgcgtca aaaaatccca gtactgcacc atgaggggct tgtcgaggcc
     9001 taactcgcgc cgggtctgct cgatatcggc cggcgtggca tcgtcgccca ggagcaagga
     9061 tacgggatct ccgggagcga tatgcatgag cataaaaacc agaaacgaaa tacccagtaa
     9121 gaccggtatc gcgccaagta ttctgcggct cagggtgttc attctttaga acattgagaa
     9181 ccagagcgtt ggggaattca ccggcaggcg atcaaagccc cagatgggaa aaagattggt
     9241 ccagcacttt gagggcatga tcgatctggt ccttattgac gttcagggcc ggtgataccc
     9301 gtacgacatt gccataggtt ccgcctttgc cgagcaacag gccatggttc ttggtcaact
     9361 ccatgagccg cttcgtgctc tcggcatccg gttttttctt ctcgccgaca agctccattc
     9421 cctgcatcaa gcccatgccg cgcacatcgc cgatgatcgg gtatttgtcc ttgagcgcat
     9481 ttaaaccttc gcgcaaacgg ttgcccatca cccgggcatt ctccaccagg ttgtcctctt
     9541 cgatgacgct gatcgtggca tgtgcggcgg tgcatgtgac cggattgccg ccaaaagtcg
     9601 aaatcgtgtt gccctgcatg ctgtcggcga cttccggcgt ggcaatcgtc gccccgatcg
     9661 gtacgccgtt ggccatgcct ttggcgaagg tcatgatgtc gggttcgacg ccccaatgtt
     9721 cgatgccgaa catcttgccg ccggtgcggc cccagccggt ctggacctcg tcgcagataa
     9781 aaagcccgcc gtatttcttg acgatgccga cgatctcctt gaaatattct ttgggagggg
     9841 tgataaaacc gccgacgcct tgaatcggtt cggccaaaaa agcggcgatc cgcccctgcg
     9901 atgtcatcgt ctgaatcgcc tcttcgatgt ccgtggcgca tttgatattg cagctcgggt
     9961 aggtcagtcc gaagggacaa cggtaacagt aggcgttggg aatgtggtgc acgccgggca
    10021 cgacgttggg cgttaggcgc caactcgatt gcgccgacag actcagcgcc agatacgaac
    10081 ggccgctata actatggcgc agcgcgatga cgtcctggca cttggtatgg atctgcgcca
    10141 gcaatacggc ggtctcatta gcttccgtgc cgctgttggt gaaaaatgat ttttctaacc
    10201 tgcccggagt gatttgcgcc agcttctcgg ccagctgaac ttgcggctcg ttggcgtaca
    10261 aagtcgacat atgctgtagc ttgtggcttt gttcatcgat cgctttggtg accctttcgt
    10321 tacaatggcc gacgctaacg gtgagaatgc cgccaaaaaa atccaagaat tctttgccgt
    10381 cggcatcata aacgtagtga tctttggcgt gatccaccac caaaggctgg tcgtaatagg
    10441 tggcgacaca ggaaaaaaga tattttttct gtttgtcgag gatttcttgt ttgttcattc
    10501 tttcaaccaa ctctcatcat gttatctaca ttccgaaaga tgtctctgcg gagcagcctt
    10561 gttgccgctc gtaggagttt ggcacaggtt gatcaaaaag gtctcagagg ctaggcgcgc
    10621 gatgactcga cgaacggagg cgtactgttt cagtacgttg acgtgaggcg atcgagcgca
    10681 acgaagcata tgagatcttt ttggtcaggc tgttagtggc gtcgcaggaa atcgatgatc
    10741 ggcggatgcc ccttttccgg catctctctt agataaccgt gccttccacc ttcgaccaga
    10801 acaaactcgg agccggcaat cttgtccgcc agcgctttgg ccgaatcaac gtggctgccg
    10861 gtgccgcctt cctgagtatc tttgctgccg acaatgacca gagtcggcgc cttgattctg
    10921 tcgacgatgt cggtggtctc atgacactgc cgcgcgacta catgccgcaa atagcacttc
    10981 aagggcggga cttcatcgac gatcaactcc tgatattttt gcacgacgtc cgggtgctct
    11041 ttcataaact gttcgctgaa cataaagccg ggaccccagt ggtcatgctg gtatttctca
    11101 taaccctttt caatcatttc cagggcggca tccagcggca cgccgcgcgg atagtcttcg
    11161 agcaggtcgc tgtactttcc cgagccgcta ccggatagca caaggctgcg gattttttcc
    11221 ggataatcca gagccagcca ctgggctacg cggccgccca tggaatgacc cataacgtgc
    11281 gcttttttaa tgcccagcgc atccatcaaa ccggcgcagt ctttggcaaa cagacgggta
    11341 gagtagtgaa cgtccggctt atcgctgcgg cccaggccgc gatgatcgta aatcagaact
    11401 tgataatgct tggagaactc cggcacctga gcgatgcgcc acggcgctag gctgatgccg
    11461 gtgccggcaa ccagcaccag cggatctccc tggccataaa gctcatagta aaggttgacg
    11521 ccgtcgacat tcaccttgcc gctcctgacc ggcttgattt catccatgct ggaactcctt
    11581 ccgcttaccg cccgtataca gaatagccac gcaaacgagc atcgagacgc tatccaataa
    11641 agaaattaga gtcaacaaag caccgacggt acgagcggct gaatcgaagc ggatgatggt
    11701 ccacctgtgc gtagctcttt gacacagaaa gtttgcgcca ggcccatgag attgacagcg
    11761 aatcacccct tgagataatc cggagccgcg ggcgcgagca ttggcgaatc actaaggaga
    11821 gcttgatgaa gcggattatc atcttgatcc tgtgccttaa ctcgatcg
//
LOCUS       DASUVS010000488        20024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1087961, whole genome shotgun sequence.
ACCESSION   DASUVS010000488 DASUVS010000000
VERSION     DASUVS010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 20024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20024
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087961"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1684
                     /locus_tag="VF182_25495"
     CDS             122..1684
                     /locus_tag="VF182_25495"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEX6440499.1"
                     /translation="MQNFHTEAAWAYHDATKHSYQSIRAKPHYLDWDNQPLAFKIYSN
                     LPAISLPQNLSSSGMPALSAISTAADVATSEVRVNLQTLAEILYFSAGITRQRKYAGG
                     EMLFRAAACTGALYHIDLYLVCGELDGLDAGVYHFSPQDFSLRQLRQGDYQSALVEAS
                     GEERAIAQASCILVSASTFWRNAWKYQARAYRHCYWDNGTILANLLAIAAARDIPAKL
                     VLGFIDATVSQLLALDPQREAPLSLVALGRWSKKKITLSPVIEPLVFETIPLSKSEIE
                     YSDMRRMHEASSLQSGEEVAAWRRRQYGNDKTTEDQGKSEARLFPLQSLSDQGLPKES
                     IETVITRRGSTREFSRESITFVQLSSMLDRATRGVSTDLLGAEETLNDLYLIVNAVEG
                     LPSGAYVFRRQRRALELLKPGDFRGDAGYLGLGQQIPADCSVNIYFLADLHRILERYG
                     NRGYRAAQLEAAIIGGKIYLGAYAQRLGASGLTFFDDDVTEFFSPDATGKSVMFLVAV
                     GKSVKRKNPSAT"
     gene            complement(1755..2573)
                     /locus_tag="VF182_25500"
     CDS             complement(1755..2573)
                     /locus_tag="VF182_25500"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6440500.1"
                     /translation="MKRFCEQRISRIIFAAAVLISTLLGLTRSTHSSCRDPEVAGSFG
                     SFAYAANVDATIQYFGHNFFLITTSKGTRIVTDPLGPGWYPNPNVVGDVVTVGKEQFN
                     HNAVQIVLGNPVILRGLKTYGAGWNTISTSIKDAFIYNVPIHQNAEFIQGIHGSAFVF
                     DLGALCIAHLGDLSQKLNDQQVKAFGKVDVALTPIGGRRTMDPELAREVLAQLKPKIA
                     IPMHHRDNPSLVQQFSAGMRMQVLPGDTLMLSKSSLPQATEIKVLQPRGAMSYQ"
     gene            3007..3990
                     /locus_tag="VF182_25505"
     CDS             3007..3990
                     /locus_tag="VF182_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6440501.1"
                     /translation="MKFTRGLGIVISTAFIIQLLLGQSIAIAWEPTKPIEFVIPAGTG
                     GGADQMARLMAGISDKHKLSPRPFIVVNKSGGAGAEGFLHVKEKKGDAHTIIITLSNL
                     FTTPLATGTPFSWRDFTPVARMALDRFILWVNAETPYKSAKDYIGAVKQKPGMKMGGT
                     GSKQEDQIITVLLEQSQGVKFTYVPFKGGGEVCTNLVGNHIDSTVNNPAECVSNWKAK
                     RVRPLAVFDPERIPDKEWSEIATIKEALGANISYNMLRGIFGPADMPKEALEWHIGLL
                     KKIFETKDFQDYLHKGALKPAFATGSEYVKWVEENDKLHKELMTKGGLVKK"
     gene            3992..4471
                     /locus_tag="VF182_25510"
     CDS             3992..4471
                     /locus_tag="VF182_25510"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX6440502.1"
                     /translation="MRAVDSMTAALLMLLSAAVIYQALRLGAGWGSEGPESGFFPFWL
                     AILLAMVSIVLFVQALRIRSQEPFVTRKQLVPVLKVLGPLAGFVLITDPPGPWSGLGL
                     YVAAGLYLGFYMRWVGGHDWRTVVALATLIPVLTFLIFETWFLVPMPKGPVESWFGY"
     gene            4475..5992
                     /locus_tag="VF182_25515"
     CDS             4475..5992
                     /locus_tag="VF182_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6440503.1"
                     /translation="MGLENLLLGFQVAVTPYNLFIAVVGILLGTIIGVLPGLGGANGV
                     AILLPLTFTMPPTSAIILLTCIYWGALFGGAITSVLFNIPGEPWSVATTFDGYPLAKD
                     GKSGEALTAAFTSSFVGAFFSIVLITFFAPLLAEIALKFGPPEFFAIQLLTFSSFVGL
                     GGGNPLKSLATILIGFILSTVGLDIVTGQLRLTFGFTDLMKGFDFIIAVIGLFGIGEI
                     LLSVEEGLRFQGAKTDMNMRVVLDTWKVLPRFYRTFVRSSIIGFWMGFKPGGATPASF
                     MSYAFAKRFSKHPETFGKGEIEGIVAPETAAHAAGVAAMLPMITLGIPGSPTAAVMLG
                     GLIIWGLQPGPMLFVDNPDFVWGLIASMYTGNVIGVLIVLAFVPFFAAILRIPFAILT
                     PLIVVICAVGSYAVHNSMIDIWYMVIFGVIGYLFKKLDYPLAPLVLALVLGDMAENAL
                     RQSLIMSQGSLMIFLIRPISGVITAVAIFFFILPLLTPWWRKMRGLPQQRKPSLD"
     gene            complement(6162..8324)
                     /gene="fdhF"
                     /locus_tag="VF182_25520"
     CDS             complement(6162..8324)
                     /gene="fdhF"
                     /locus_tag="VF182_25520"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX6440504.1"
                     /translation="MITPDRIVPTTCPYCGVGCNLELHIKDDFVYKVTSPFEAVVNHG
                     NLCVKGRFGYDFIYNKERITTPLIRKTAQEPGRRTQAFDRDQWREVSWDEALDYAAGR
                     LVEIYRRDGADAMAVYCCAKATNEDNYLLQKLFRALFRSNNVDHCTRLCHAGSVVALQ
                     MALGSSAMSNTAAEVIHSDVFLITGSNTAETHPIIALQMKAAVANHGAKLIVVDPRRV
                     EMVNWATLWLPEKPGTDVPLFSAMAHVIIKEQLYNRDFIERRTEGFDEFVRSMEKFTP
                     AYAEAISGVDRNLIIEAARMYATAKNAAIYWALGIPEHSHGTDNAMSLIHLALLTGQI
                     GRKGTGLNPLRGQNNVQGASDSGAMPWHYPGYQRVDDEAAANKFERIWNSEPGSLNRK
                     LGLTTTEIMSAVGPGGVRALHIMGENPMMSEPNLNHTRHKMEELEFLVTQDLFINESG
                     AYADVFLPAASWAEKEGTFTNTDRRVQRVRQALEPRGQARPDWQIICDLAARIEQKLG
                     RAQTAFWDYESPAEVLEEMGSVVPEYAGVKYRRIENQGLQTPVWDDNHPGTPYLFAET
                     FPSGKGRFHPLEYVPCVEMPDDEYPFILTTGRLLEHWHGGTLTRHSKLDELYPEARIE
                     INPADAARLKIEDGQTVRVSSRRGSIVLRAWVTPRATVGVVFIPMHFAEAAANLLTID
                     ALDPLAKIPEYKACAVRVVPATEDQLARPDERIQRGRY"
     gene            complement(8321..9022)
                     /locus_tag="VF182_25525"
     CDS             complement(8321..9022)
                     /locus_tag="VF182_25525"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX6440505.1"
                     /translation="MADITLTIDGESVTVPDGTTILKAAEKAGRQIPTICYHDHCTAN
                     GLCRICVVEVDGVRPLVPACLSRASEGMKVTTSSARAERSRRTILEMLASAVDLSEAP
                     EIQKFIDAYRADTARFPDAERREYPVLDDNPMYLRDYSKCILCWRCVQVCAEDAQYTY
                     AINFKGRGFETGIGTFFDKPMPETTCVFCGQCVGVCPTGALKPKRESLLEQGLSLDEI
                     MQASRGGKKRRRKSP"
     gene            complement(9104..10828)
                     /locus_tag="VF182_25530"
     CDS             complement(9104..10828)
                     /locus_tag="VF182_25530"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX6440506.1"
                     /translation="MSDADFSRMQSILIGLAPQGRAALLPALHLAQQIHGWLPEAVAA
                     EVGRVLGVPLADVHGVIDFYEMLSRQPIGRTVARVCAAPVCALAGGDGVADALCSYFK
                     VEPGQVSADGEFTVEHAPCLGLCDHAPALLLGESAIGRAAPEQPDKISARSIERPISF
                     VGGDIRMLTSNCGQGQCTSLADYSSGGGYAGLKKALTMAPQDVIAEVKSAGLVGRGGA
                     AFPTGIKWEGAFKASGQPKYIVCNADESEPGTFKDRILMEEDPHRTLEGMIIAAHAVG
                     ARHGYMYIRGEYPYAFKIMSEAVDEARQAGIIGKNIFGSGLDFDIEMRLGAGAYICGE
                     ETALLESIEGKRGFPRIKPPFPTTHGLFGKPTVINNVETFCNIPLILEGGAVEYRKIG
                     TERSPGPKLFCVSGQIQKPGLYEVPFGVTLRHLLFDLAGGLNQGHKLHAVLMGGAAGA
                     FATEKDLDVILSFENLSATGLPLGSGAVMVFDDTADLRDVLKRLAHFFADESCGKCYP
                     CQLGTQRQFEILDRAAAGAAAAGDRECLSDVGGTMIDASLCGLGQTAATAVLSAMKVW
                     PELFERKK"
     gene            11065..11829
                     /locus_tag="VF182_25535"
     CDS             11065..11829
                     /locus_tag="VF182_25535"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX6440507.1"
                     /translation="MLRAIKRIRIHEEVFKQIRQLIEEGRFRARDQLPSERELAETFK
                     VSRTSVREALRTLETQGLIVSRTGTGNFVADLPVESLIGPLARLLIDEKKALADMFEM
                     RKLIEPHIAALAAERATRNDVAQLKRILAKQSTAVSRGETGVEADAELHFFISRTTRN
                     HALQKLVSGLMELLSRSREESLQTDNRRATSIAAHRRIIAAIGRHDKAKARAEMLRHI
                     EQVEESVLSTSRLLKNDRQNSTRDRGVLREQASRIG"
     gene            11931..13643
                     /locus_tag="VF182_25540"
     CDS             11931..13643
                     /locus_tag="VF182_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6440508.1"
                     /translation="MAENSESKKEATGPVISGGHLVAKALKNEGVEVIWTLCGGHIID
                     IYDGCIDEGIKIIDVRHEQVAAHAADGYSRQSGKIGCVVTTAGPGTTNAMTGIANAFR
                     AESPLLMIGGQGALSQHKMGSLQDLPHVDFMSPITKFAASVPHTERIADMVSMAFRES
                     VNGAPGPSYLEIPRDVLDAKVPLERARVPKPGAYRASTKSVGDPVDIERLADILVKAE
                     RPAALFGTQVWTCRGHDAAVEFCRALNIPGYMNGSSRGIFAPGDPHGFNRTRRLAFDK
                     ADVILIVGSPFDFRLGYGRRLRADATVVQIDLDYRNVGKNRDISLGLVGDCGAILKAV
                     EQASSSRASKGNAQRAEWMKELRRAEAKAYEKLLPSFKSESTPINPYRVAYEINEFLT
                     DNTVYIGDGGDVVTITATAVEPRKAGNWMDPGPLGTLGVGTSFAMATKFVHPKKEVLC
                     YYGDGSFGMTSWDMETAQRFGLPYLAVIGNNSAMNQIRCGQIGKYGPERGCVGNKLGD
                     VDFEKFADCFGGWGIAVREPGKIRPALEKARERVAGGQCAIVNIWVDINEYAPGTKAQ
                     TMYK"
     gene            13780..15021
                     /gene="frc"
                     /locus_tag="VF182_25545"
     CDS             13780..15021
                     /gene="frc"
                     /locus_tag="VF182_25545"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEX6440509.1"
                     /translation="MSKALDGIRILDMTHVQSGPTCTQILAWFGADVIKVELPGRGDI
                     TRGQLRDKPNVDSLYFTMLNCNKRSITLNTKNEKGKEIFRKLIKACDVMVENFAPGAL
                     DRQGFTWEVIRELNPRMIYASVKGFGPGPYEDCKVYENVAQCTGGAASTTGFDDGPPL
                     VTGAQIGDSGTGIHLVAGILAALFQREKTGKGQRVTCAMQDTVLNLCRVKLRDQQRLA
                     AGPLMEYPQYPNGVFGDAVPRAGNASGGGQPGWAVRTKGGGPNDYIYVIIQPPGWEPL
                     MKLCGRPELITDPEWATPEKRLPKLKRCFEIIEQWAMTLDKLEVMRKLNEFDVPCGPI
                     LDMREIAAEQSLRDTGTVVEVDHPKRGKFLTVGNPIKLSESRTEVRASPLLGEHTDEI
                     LKSVVGWSETEIAAARKEGAI"
     gene            15258..17378
                     /locus_tag="VF182_25550"
     CDS             15258..17378
                     /locus_tag="VF182_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX6440510.1"
                     /translation="MKNENLRATINSAFAAGRRALSAPEAQQLCEAYGISTPRQALAT
                     TASEAAEVAAKLGFPVVLKIVSDDILHKTEAGGVIVGLENAADVRAGFERLVSNAKQY
                     KSNASIQGVQVQQMLKGGQEVMVGAVTDATFGKMIAFGLGGILVEVMKDITFRMAPVS
                     ETEALAMLDSIAAAEVLRGVRGGKGVNRAALADIIAKVSKLVSDFPEINEVDLNPIFA
                     TETGATAVDVRIVLGEPPKARQRYTQSEILAAMQRIMNPRSVAVIGASAEDGKIGNSV
                     MKNLINGGYQGTILPIHPKASEILGRKAYPSVLKVGEEIDVAVFAIPAKFCAAAMEEV
                     GQKKIPGAVMIPSGFAEIGEQALQDELIGVARKHNVRVMGPNIYGFYYTPQNLCATFC
                     TPYDVKGKVALSSQSGGVGMSIIGFSRSTKMGVSAIVGLGNKSDLDEDDLLTYFEQDD
                     NTQVIAMHCEDLKDGRSFAEVASRVSKTKPVVVLKAGRTALGARAAASHTGALAGNDK
                     IYDDVLRQCGVVRAKSLNDMLQFARGLQVLPNPKGENVVIITGAGGSGVLLSDACVDN
                     GLSLMKMPPDLDAAFRKFIPPFGAAGNPVDITGGEPPTTYANTIRYALGEPRIHSLIL
                     GYWHTIITPPMVFAKLCAEVVQEARDQGIDKPVVASLVGDVQVEEASEYLYERGIVAY
                     PYTTELPVAVLGAKYKWARGAGVL"
     gene            complement(17602..18876)
                     /locus_tag="VF182_25555"
     CDS             complement(17602..18876)
                     /locus_tag="VF182_25555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6440511.1"
                     /translation="MRLNFSASSPLWRSLRHRNYRLYLTGQLVSVCGTWMQQVALSWL
                     VYRLTGSATLLGVVGFASQIPILVLGPIGGVINDRYSTHRVAVWTQCGALAQALLLAL
                     LALTGWIQPVHIILLGMALGIVYAFDMPARQALVHRLVDAEDLPNAVALNSSMINAAR
                     IVGPALAGLVVARFGEGICFVINAASYIATITALLKMKFIEPPCRDATVTSIGRSLIE
                     GYRYTTATTPIRDLLMLLGVVGVMGMPYMTLMPVFAAQVHGSGAGTLGIMFGAVGFGA
                     LIGALFLAQRKNIIGLGRVIVFATLGFGMGLVCFTLAKIFWLSLLLLVAVGYGWMVLI
                     AASNTVLQTLADNQMRGRVMSLFSMMLVGMAPFGSLLAGWAADQVGAPMVVAIGGGFC
                     AIAGIVFARRLPGLREAAKPILAARGIILEAK"
     gene            complement(19055..19366)
                     /locus_tag="VF182_25560"
     CDS             complement(19055..19366)
                     /locus_tag="VF182_25560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhR family protein"
                     /protein_id="HEX6440512.1"
                     /translation="MVTAVVQFKIPTPMSREQAKEIFTSTAPKYQAARGLIRKYYLLS
                     EDGGTAGGVYLWQSREDAESLYTKEWKSFVKEKYGAEPAIVYFQTPVVVDNLTKEIIE
                     D"
     gene            19774..19974
                     /locus_tag="VF182_25565"
     CDS             19774..19974
                     /locus_tag="VF182_25565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015225751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX6440513.1"
                     /translation="MKLKVVLEPSDEGGFIVYVPSLPGCVSEGESETEALENIREAVE
                     LYLEPVDDDLAGVGKEVREIVL"
BASE COUNT         4284 a         5585 c         5780 g         4375 t
ORIGIN      
        1 gcggcaaacc ggacgctgat cgctggcgcc gcatcgatac acttgctacg agtaaagaga
       61 tcgagctact cggggaagtg gaccggatgt ccgctgatga ctaatcgttg aaggagtttc
      121 gatgcaaaat tttcacactg aagccgcatg ggcttatcat gacgcgacca aacactcata
      181 tcagagtatc cgggccaagc cacattactt ggattgggac aaccagcccc tggcttttaa
      241 aatttactcc aatctgccgg ctatttctct gccgcaaaac ttatcatcct ccgggatgcc
      301 cgcgctttcg gcgatttcaa ccgccgcaga cgtagcgacc agcgaagttc gagtgaatct
      361 acaaactctg gcggaaatcc tttatttctc cgcgggcatc accagacaga ggaagtatgc
      421 cggaggagaa atgttgtttc gtgcggcggc atgcacgggc gcgctctatc atatcgattt
      481 gtatcttgtt tgcggcgagc tggacggcct ggatgcgggc gtctaccact ttagcccgca
      541 ggatttttca ctacggcaat tgcgccaagg ggattatcaa tcggccctgg tggaagccag
      601 cggcgaggaa cgggcaattg cacaagcctc ctgcatcctc gtgagcgcct cgaccttctg
      661 gcgcaacgcc tggaaatatc aggctcgtgc gtatcggcac tgttattggg acaatggaac
      721 catactggcc aatctgcttg ccattgcggc ggctcgcgat attccggcaa agctcgtcct
      781 gggtttcatc gatgctacag tgagccaact cttggccttg gatccgcagc gggaggcgcc
      841 gctttcgttg gtggcgttag gccgttggtc caagaagaag atcactctct ctccagttat
      901 cgagccgctg gtttttgaaa cgattcccct gtcgaagtcg gagatcgagt attctgacat
      961 gcggcggatg catgaggcgt cctcgttaca aagcggggag gaggttgcag cctggcgtag
     1021 gcgtcaatac gggaatgaca aaacgacaga ggatcaaggc aagagcgaag ctcggctttt
     1081 cccgcttcag tcactcagtg atcagggact acccaaagaa tccatcgaga cggtgataac
     1141 ccggcgcggg tcgacgcgcg aattttcgcg cgagtcgatt acgtttgttc aactttcgag
     1201 catgctcgat cgcgcgacac gcggtgtatc gaccgattta ctcggcgcgg aagaaactct
     1261 taatgatctt tacctgattg ttaatgccgt ggagggtctg ccttccggcg cctatgtctt
     1321 tcgtcgccag cggcgcgcgc tcgaattatt gaagccgggc gattttcgcg gcgatgccgg
     1381 ttatctcggc ctcggccagc aaatcccggc ggattgtagc gtaaatattt actttctggc
     1441 tgatttgcat cgcatcctcg agcgttacgg caatcgcggt tatcgcgccg cgcagctcga
     1501 ggccgctatc atcggcggga aaatttatct tggcgcttac gcgcaacgcc tcggcgcctc
     1561 cgggctgacg ttcttcgacg acgatgtgac cgaatttttc tcgcccgatg ccaccggtaa
     1621 gagcgtgatg ttcttggttg ccgttggaaa atcagtcaag cgaaagaatc cgagcgcgac
     1681 ttaacgtgaa atcaggtgat gtccaccgcg ctgcgttcgc atcccacgaa cacgatcact
     1741 tcttagacga gagattactg atagctcatt gcgccgcgcg gttgcagcac tttgatttcc
     1801 gtcgcctgcg gaagcgagct tttgctcagc atcagcgtgt cgcccggcaa tacctgcatt
     1861 ctcatgcccg cgctaaactg ttgtacgaga gatggattgt cgcggtgatg catcggaatg
     1921 gcgatctttg gtttgagctg agccaggact tcgcgagcga gttccggatc catcgttcgc
     1981 cggccgccaa tgggtgtgag cgccacatcc actttgccga acgctttgac ctgctgatcg
     2041 tttagctttt ggcttaaatc accgaggtgc gcaatacaca aagctcccaa atcaaaaacg
     2101 aaggccgagc catgaatgcc ctggataaat tcagcgttct ggtgaattgg cacattgtaa
     2161 atgaaggcgt ctttaatgct cgtgctgatg gtattccagc ccgccccata cgttttcagt
     2221 ccgcgcaaaa tcaccggatt gccgagaacg atctgaaccg cattgtgatt aaactgctct
     2281 ttaccgacgg tgacaacgtc tccgacaacg ttcgggttgg gataccaacc gggtcccaga
     2341 gggtcggtga cgatgcgcgt tcccttgctg gtggtgatga gaaagaaatt gtggccgaag
     2401 tattggatcg tagcgtcgac attcgccgca taagcgaaag atccgaagct gcctgcaact
     2461 tccgggtcgc gacaagaaga atgagtggaa cgggtaagac ctagcagggt gctgatgaga
     2521 accgccgcag cgaagataat gcgtgaaatt ctttgttcac aaaagcgctt catgagatac
     2581 catagctcgt ttatgcagtc gcgcgtaaca ggacgttatt ggatcgaagc agaaaaaaca
     2641 atcgtcggac cgcttcatct tgatgctcgc gcaaatcggc ctgagcctgc gagcgtttcg
     2701 attgtgaggt ggcgttaatc gggaaccggg ttcagcctgc aatacctcta aacaaagaag
     2761 agaatcggct cggtaccagg gaacattgga atcgtgtttt ggtaagtatc cttgagcctc
     2821 atcggcgggt gttggctctc tcttatgttg tgtaatgccc gtgatccgtc atcagatcgc
     2881 cttggcctgt cttgacacta gctcaaattc tgattacccc tactcaccaa acacggcgag
     2941 atgtggcagc tttgaagcag gaacctgagt ctaattggat caaagctgaa ccagaaaggg
     3001 ggttctatga agtttacaag aggcttgggt attgtgatct ccacggcttt catcattcag
     3061 ctccttcttg gacaatccat agcaatcgct tgggagccca ccaagccaat tgaatttgtg
     3121 ataccggccg gcaccggtgg cggcgccgat caaatggcgc gtttgatggc cggcatcagc
     3181 gataaacaca agctctcgcc gcgacctttc atcgtggtca ataaatccgg cggcgccggc
     3241 gccgaaggct ttttgcacgt gaaggaaaag aaaggcgacg cgcacacgat catcattacc
     3301 ttgtcaaacc ttttcaccac accgctggcc accgggacgc cgtttagctg gcgcgatttt
     3361 acgccggttg cccgcatggc gcttgaccgt ttcattcttt gggtgaatgc cgaaacaccc
     3421 tacaagagcg ccaaagacta cataggcgct gtgaagcaga aaccgggcat gaagatgggc
     3481 ggcacgggat ccaaacagga agatcagatc atcacggtcc tgctggagca aagccagggg
     3541 gtgaagttca cttacgtgcc cttcaaaggt ggcggtgagg tttgcaccaa tctggtcggc
     3601 aatcatatcg actcaacggt caataacccg gccgagtgcg ttagcaactg gaaggcaaaa
     3661 cgtgtccggc cgctcgcggt gttcgatccg gagcgtatcc ctgacaaaga atggagcgaa
     3721 atcgcgacaa tcaaagaggc actcggcgcc aatatctcgt acaatatgct ccggggcatc
     3781 ttcggccctg ccgatatgcc caaggaagcg cttgaatggc acatcggcct tctgaagaaa
     3841 atttttgaga ccaaagactt tcaggattac ttacacaagg gggctcttaa accggccttt
     3901 gccaccggta gcgagtatgt caaatgggta gaagagaatg acaaactcca caaggaactg
     3961 atgacgaaag gcggtttggt caagaagtag aatgcgcgct gtcgatagca tgaccgccgc
     4021 gctgctgatg cttttgagcg cagcagtgat ttaccaagct ctgcggctcg gcgccggttg
     4081 gggcagtgaa ggccctgaaa gcggtttttt cccgttttgg ttggccatcc tactggccat
     4141 ggtcagcatc gttttgttcg tgcaggccct gcgcatccgg tcgcaagaac ctttcgttac
     4201 gcggaagcag ttggttccgg ttctcaaagt tctcggacct ctggccggat tcgttctcat
     4261 aaccgatccg ccgggcccat ggtccgggct cggactgtat gtcgcggcgg gtctttatct
     4321 cggtttttac atgcgctggg tcggcggtca cgattggcga acggtggttg cgctcgccac
     4381 attgatacct gtcctcacct tcctgatttt cgagacctgg tttctggtgc ctatgcccaa
     4441 aggaccggtc gagagttggt ttgggtacta attcgtggga ctcgagaatc ttcttcttgg
     4501 cttccaggtt gccgtaacac cttataatct gtttatcgcc gtggtgggga tactccttgg
     4561 caccatcatc ggtgtactgc ctggactcgg aggcgccaac ggcgtggcga tcttgctgcc
     4621 gcttactttc acgatgcctc ccacttctgc gatcatcctg ctcacctgta tttattgggg
     4681 cgctttgttt ggcggcgcga tcacctcggt gcttttcaat attccgggcg agccgtggtc
     4741 ggtggcgacg acgttcgacg gctatccgtt ggctaaggac ggcaagagcg gcgaggcgct
     4801 tacagccgca ttcacttcat cgtttgtcgg tgccttcttt tcgatcgtgc tgatcacgtt
     4861 ttttgcgccg cttctcgccg agattgcgct gaagttcggc cctcccgagt ttttcgccat
     4921 ccagcttctg acgttttcaa gtttcgtcgg gctcggcggg ggtaatccgc tcaagtcgtt
     4981 agcgacgatt ctcatcggct tcatactttc cacggtgggg ctcgatatcg tgaccggcca
     5041 gctcaggctt accttcggtt tcaccgatct gatgaagggg tttgacttca tcattgccgt
     5101 catcggtctg ttcggcatcg gcgagatttt gctcagcgtg gaagaggggc tgcgctttca
     5161 aggcgccaag accgatatga acatgcgcgt cgtgctcgat acctggaagg tcttgccgcg
     5221 cttctaccgc acgtttgtac gcagctccat catcggtttc tggatgggtt tcaagccggg
     5281 tggcgcaacc ccggcctcct tcatgagcta tgcctttgcc aagcgcttct caaagcatcc
     5341 agagacattc ggcaagggcg agatcgaggg cattgtcgct cctgaaaccg ccgcccacgc
     5401 ggcgggcgtt gcagcgatgc tgccgatgat cactcttggc atcccgggtt cgccaaccgc
     5461 agcggtcatg ctcgggggat tgatcatttg gggcctccag cctggcccga tgcttttcgt
     5521 cgataacccc gactttgtct ggggcctgat tgccagcatg tacacgggaa acgtaatcgg
     5581 cgtcctgatc gtgctggcgt tcgtgccgtt cttcgcggcc atcctgcgca ttccgtttgc
     5641 gatcctcacg ccgctcattg ttgtcatctg tgcggtgggc tcttatgccg tccacaacag
     5701 catgatcgac atctggtaca tggtgatttt cggcgtgatc ggctatcttt tcaagaaact
     5761 cgattatccg ctggcgccgc tggtactggc gctggttctc ggcgatatgg cggaaaacgc
     5821 gttgcggcag agtctgatta tgtcgcaagg ctcactgatg atttttttga tccggccgat
     5881 atcgggcgtg atcacggccg tcgccatttt ctttttcatt ttgccgctat tgacaccctg
     5941 gtggcgtaag atgcgagggc tgcctcaaca gcgtaagcct tcactggact agtcataaag
     6001 agcaagaagt tcaagagttt ttgacttcaa cggcataact cttcgtggcc ttcgtgtgaa
     6061 tcgtggtgag acgtatccgc tctacgcagt aatcagcgcc tagcctgaga aaatgtactc
     6121 acagactgtg tagattctga tttggcgacg aacacttgcc gttagtatct ccccctttga
     6181 attctctcgt ccggcctggc taattggtct tcggtggcgg gtacgacgcg cactgcgcag
     6241 gctttatatt cgggaatctt ggcgagggga tccaaagcgt cgatggtcag tagattggcg
     6301 gcagcttcgg cgaaatgcat cgggatgaag accacaccca ccgtcgcccg cggtgtcacc
     6361 caggcgcgta gaacgatgga accgcgccgg gacgagaccc gcactgtctg tccatcttcg
     6421 atcttgaggc gggctgcgtc cgcgggattg atctcgatgc gcgcttccgg atacagctcg
     6481 tcgagcttcg aatggcgcgt cagcgtgccg ccgtgccagt gctcgagtag gcgtccggtg
     6541 gtgaggatga acgggtattc atcatccggc atttccacac acggcacata ctcaagcggg
     6601 tggaacctac ctttgccgct ggggaaagtc tcggcgaata gatagggggt gccgggatga
     6661 ttgtcatccc ataccggcgt ctgcaagccc tgattttcga tgcgccgata ttttacgccg
     6721 gcgtattcag gaaccacact ccccatctct tcaagcactt cggcggggct ctcgtagtcc
     6781 caaaaggctg tctgcgcgcg gccgagcttc tgttcgatgc gcgcagcaag atcacaaatg
     6841 atctgccaat cgggtcgggc ttgcccgcgc ggttccagcg cctggcgcac gcgctggacg
     6901 cgccggtcgg tgttggtaaa cgttccttcc ttctccgccc atgaagccgc gggcaaaaag
     6961 acatcggcat aggcgccgga ctcattgatg aacaagtctt gggtgaccag gaactccaac
     7021 tcttccattt tatgacgcgt gtggttcagg ttgggctcgg acatcatcgg attctcgccc
     7081 atgatgtgaa gcgcccgcac accgccgggt cccaccgcgc tcatgatttc cgtggtagtc
     7141 aaccccagct tgcggtttag gctgcccggt tcgctgttcc aaatgcgctc aaatttattt
     7201 gccgccgctt cgtcatcgac tctctgataa ccgggataat gccatggcat ggcgccggaa
     7261 tccgatgcgc cctgcacgtt gttctgcccg cgcaagggat tgaggccggt gcctttgcgg
     7321 ccgatctggc cggtgagcag cgcgagatga atcagcgaca tcgcattgtc ggtgccgtgg
     7381 gaatgctcgg ggatgcccag cgcccaatag attgccgcgt tttttgccgt cgcatacatc
     7441 cgggcggcct cgatgatgag attgcggtcc accccggaaa tcgcttcggc gtatgccggc
     7501 gtgaattttt ccatggacct gacgaactcg tcgaaacctt cggtgcgccg ctcgatgaaa
     7561 tcgcggttat aaagctgttc tttgatgatg acatgcgcca tcgcggaaaa gagcggcacg
     7621 tccgtgccgg gtttttccgg cagccagagc gttgcccagt tgaccatctc aacccgccgc
     7681 ggatcgacga cgatgagctt ggcgccatgg ttggccaccg ccgctttcat ttgtagcgca
     7741 atgatcggat gggtctctgc ggtgttggag ccggtaatga gaaaaacgtc gctgtgaatg
     7801 acttccgccg cggtgttgct catggcagag gagcccagcg ccatttgcag cgccactacc
     7861 gagcccgcat ggcacaagcg tgtgcagtga tcgacattat tgctgcggaa taaggcgcga
     7921 aacagttttt gcaggagata gttgtcctcg ttggttgcct tggcgcagca gtagaccgcc
     7981 atcgcatcgg cgccgtcgcg ccggtaaatc tccaccagcc ggcctgcggc gtaatcaagc
     8041 gcctcatccc acgatacctc gcgccactga tcacggtcga aggcctgagt gcgcctgccg
     8101 ggctcctgcg cagtcttgcg aatgagcggc gtggttatcc gttccttgtt gtaaatgaag
     8161 tcgtagccga agcgtccttt gacgcacaga ttgccgtggt tcaccactgc ttcgaacggc
     8221 gaggtcactt tgtaaacgaa atcgtccttg atgtgcagct ccagattgca tcccacgccg
     8281 cagtaggggc aggtggtcgg gacgatgcga tccggtgtaa tcatggtgat ttcctccggc
     8341 gtttttttcc gcctctggac gcctgcatga tttcatccaa cgaaaggccc tgctcgagca
     8401 gcgactcgcg cttgggcttg agcgcgccgg tggggcagac gccgacgcac tggccgcaaa
     8461 atacgcaggt cgtctcaggc atcggcttgt caaagaaggt gccgatgccg gtttcgaaac
     8521 cgcgcccctt gaagttgatc gcataggtat actgcgcatc ctcggcgcat acctgaacgc
     8581 agcgccagca caggatgcat ttggagtagt cgcggagata catcggattg tcatcgagca
     8641 ccggatactc gcgccgctcg gcatcgggaa agcgcgccgt atcggcgcga tacgcatcga
     8701 taaacttttg aatctccggc gcctcagaaa gatccacggc cgaggcgagc atctccagta
     8761 ttgtccggcg gctgcgctcg gctcgcgcgc tcgaggtcgt gactttcatg ccttcgctcg
     8821 cccggctcag acacgccggc acaagcggcc ttaccccgtc gacttcgacc acgcagatgc
     8881 ggcacaggcc gtttgcggta cagtggtcgt gatagcagat cgtggggatc tgccgcccgg
     8941 ccttttctgc ggccttgagg atcgtcgtgc cgtcaggaac tgtgacggac tcgccgtcga
     9001 tcgttagcgt gatatcagcc atagtgcggt tcctgtaaaa ccggaaagct gaaatctgaa
     9061 agctgagacg aaaatttccg ctttcctgtt tccgttttca tctttacttt tttctctcaa
     9121 acaactccgg ccatactttc atcgcgctca acaccgcggt cgcggccgtt tgacccaggc
     9181 cgcacagcga agcgtctatc atcgtgccgc caacatccga taagcattcc cggtcgccgg
     9241 cagcggcagc tccagcagca gcgcgatcga gaatttcaaa ttgtcgctgc gtgccgagct
     9301 ggcagggata gcatttgccg cagctttcgt cggcaaagaa gtgcgccaat cgcttgagca
     9361 catcgcgcag atcggcagta tcgtcgaaga ccatcaccgc gcccgagccc agcggcaggc
     9421 ctgtagcgct cagattctcg aacgacaaga tcacatccaa gtctttctcc gtggcgaacg
     9481 cgccagcagc gccacccatg agcacggcat gaagtttgtg cccttggttc aagccaccgg
     9541 ccagatcaaa caacagatgg cgcaaagtga cgccaaacgg cacctcgtac aatccgggtt
     9601 tctgtatttg ccccgatacg caaaacagct tggggccggg tgagcgttcc gtccctatct
     9661 ttcggtactc tacggcgccg ccttcgagaa tcagcgggat gttgcagaag gtttccacat
     9721 tgttgatcac ggtgggttta ccaaacaatc catgggtggt cggaaacggc ggcttgatgc
     9781 ggggaaatcc gcgtttgccc tcgatcgatt ccagcagcgc ggtttcttcg ccacaaatat
     9841 aggcgccggc gcctagccgc atttcaatat cgaagtccaa tcccgagccg aaaatgtttt
     9901 tgccgatgat gccggcctgc cgcgcttcat caacggcttc gctcattatt ttgaatgcgt
     9961 aaggatattc tccgcgaatg tacatgtagc cgtggcgcgc acccacggca tgggccgcga
    10021 taatcatgcc ctcgagggta cggtgcgggt cctcctccat tagaatgcgg tctttgaagg
    10081 tcccgggttc ggattcgtcg gcattgcaca cgatgtactt gggttgtcct gacgccttga
    10141 acgctccttc ccatttgatg ccggttggaa atgccgcgcc gccgcgaccc actaagcccg
    10201 ccgacttcac ctccgcaatc acgtcttgtg gcgccatcgt caaagctttc ttcaatcccg
    10261 cataaccgcc gccgctgctg taatcggcaa gcgaggtaca ctggccctga ccacaattgc
    10321 tggtaagcat gcggatgtcg ccgccgacaa agctaatcgg ccgttctatg ctgcgcgcac
    10381 tgattttgtc cggctgttcg ggagcggcac gtccaatcgc ggattcgcct agcagcagtg
    10441 ccggtgcgtg atcgcacaaa cccaagcaag gcgcatgctc caccgtgaac tctccgtcgg
    10501 cgctgacctg gcctggttca actttgaaat agctgcacag cgcatcagcc accccatccc
    10561 ctccggcgag cgcgcacacc ggcgcggcgc aaacgcgcgc gaccgttcgg ccgatcggct
    10621 gccgcgaaag catctcgtaa aaatcgataa caccatgaac atcggccagc ggcacgccta
    10681 aaacgcggcc aacttccgcg gccactgctt cggggagcca accgtggatt tgctgcgcca
    10741 ggtgaagggc cggcaggagc gcagcgcgtc cctgcggcgc caatccgatg aggatggatt
    10801 gcatgcgact gaaatcggca tcagacatct gattatatct ctcggagcga ttgtagtttc
    10861 accgctatca tctgactttc aacatcgcgg ctctggctat gcatagtagt ccagactggc
    10921 aaggtcaagg gttcgctggt cctacacagg ttccggcttt ccgaaaagag atcgattgat
    10981 tgtggctgtc gcatgagctt aggataactt gacatgcact ttgggattcg ataactggtc
    11041 agaccagtag gccggtggta cttgatgctg cgcgcaatca aaagaatcag gattcacgag
    11101 gaagtcttca agcaaatccg tcaactgatc gaggagggca gattcagggc ccgtgatcag
    11161 ctgccctcgg agcgggagct agcagaaaca ttcaaagtca gccgaacttc ggtgcgtgag
    11221 gcgcttcgaa cgcttgaaac ccaaggactt atcgtcagcc gaaccggcac gggcaatttt
    11281 gttgccgacc tgccggttga atctctaatc gggccgctcg cgcggctatt gatcgacgag
    11341 aaaaaggctc tcgcggacat gttcgaaatg cgtaagttga tcgagccgca cattgccgct
    11401 ttggcggctg agcgggccac ccggaatgac gttgcgcaac tcaaaagaat tctcgccaag
    11461 cagagtacgg cggtaagccg cggcgagacc ggcgttgagg ccgatgctga gctgcatttc
    11521 tttattagcc gtaccacccg gaatcacgct ttgcagaaac tggtctcggg attgatggag
    11581 ctgttgagcc gcagccgtga ggagtcattg cagaccgaca atcgcagagc aacctcgatc
    11641 gcagcgcatc gaagaatcat cgccgcgatc ggaaggcacg acaaggctaa agctcgagcg
    11701 gaaatgttgc gccacatcga acaggtagaa gaaagtgtgc tgtcgacaag ccggttgtta
    11761 aaaaacgaca ggcagaatag tacgcgagat agaggcgtac tgcgtgagca ggcgagtaga
    11821 atcggatagc cgccggtatt cgaacgagtg gttgcgcgcg gagatccgcg cagaaccaca
    11881 gcatcgaagc ggcaagatca caatttaacc cgagagcaag cgaggtaatg atggcagaga
    11941 attctgaatc caaaaaggaa gccaccggac cggtcatctc cggcggccat ctagtcgcca
    12001 aggcgcttaa gaatgaaggc gtcgaggtga tctggacgct atgtggcggt catatcatcg
    12061 atatctatga cggctgcatc gacgagggca tcaagattat cgacgtgcgc catgaacagg
    12121 tggcggcgca cgccgccgat ggttattcgc gccagagcgg caaaatcggt tgcgtggtta
    12181 cgactgcggg gcccggcacc accaacgcga tgaccggcat tgccaatgcg tttcgcgctg
    12241 aaagcccgct tctcatgatt ggcgggcagg gcgcgctgag ccagcacaag atgggttcgc
    12301 tgcaggattt gcctcacgtc gatttcatgt cgccgataac caagtttgct gccagcgtgc
    12361 cgcacacaga acgcatcgcc gatatggtgt cgatggcctt ccgggaatcc gtcaatggcg
    12421 cgccggggcc gagctatttg gaaattcccc gtgatgtcct cgatgccaag gtgccgctgg
    12481 agcgggcgcg ggttcccaag cccggcgcgt accgcgcatc gactaaatcg gtgggcgatc
    12541 ccgtcgatat cgaacggctc gccgatattc tagttaaagc cgagcgtccg gccgcgttat
    12601 tcggcacaca agtttggact tgccgaggac atgacgcggc ggtggagttt tgccgcgcgc
    12661 tcaatatccc cggttatatg aacggttcgt cgcgcggtat tttcgctccc ggcgatccgc
    12721 atgggttcaa ccgaacgcgt cggctggcgt tcgacaaggc cgacgtgatc cttatcgtcg
    12781 gcagcccctt cgattttcgt ctcggttatg gccgccgctt gcgcgccgat gcgacggtcg
    12841 tgcaaatcga tttggactac cgcaatgtcg gcaaaaatcg ggatatctcg ctggggctcg
    12901 tcggtgattg cggcgcgatt ctcaaggcgg tcgagcaagc gtcatcgagc cgtgcatcga
    12961 agggcaatgc gcaacgcgct gagtggatga aagaattgcg ccgcgcggaa gccaaggcgt
    13021 acgagaaact gctgccgtcg ttcaaatcgg aatcaacgcc gatcaaccca tatcgggttg
    13081 cctatgagat caacgagttt ctaaccgaca acaccgttta catcggcgat ggcggcgatg
    13141 tcgttaccat cacggccacc gcggtcgaac cgcgcaaagc cggcaattgg atggatcccg
    13201 ggccgctggg tacgctcgga gtcggcactt cgttcgcgat ggcgaccaag ttcgtgcatc
    13261 ccaagaaaga agtgctgtgc tactacggcg acggctcctt cggaatgacc tcgtgggaca
    13321 tggagacggc gcagcgtttc ggcctgcctt atcttgccgt gatcggcaac aactcggcga
    13381 tgaatcaaat ccgctgcggc cagatcggca agtacggtcc cgagcgcggc tgtgtcggca
    13441 acaaactcgg cgacgtcgat ttcgagaaat tcgccgattg cttcggcggg tggggtattg
    13501 ccgtgcgcga gcccggcaag atccgccctg cactggaaaa agcgcgcgaa cgcgttgccg
    13561 gcggtcaatg cgccatcgtc aacatctggg tcgatatcaa tgaatacgcg ccagggacca
    13621 aggcgcagac gatgtacaag taagacacag ccggacgtaa agagaggtct caccacgaag
    13681 atcacgaagg acacgaaggg ttcaacttat cagatctctg cacttcgtga tcttcgtgtc
    13741 cttagtggtg aaagcaaagt tttcgcagaa aggtaagcga tgagcaaagc gctcgacgga
    13801 attcgtattc tcgatatgac gcacgtgcag tcggggccga cctgcacgca gattttggcc
    13861 tggttcggcg ccgatgtcat caaagtggaa ttgccgggac gcggcgacat tacccgcggc
    13921 caattgcgcg acaagcccaa tgtcgacagc ctctatttca ccatgctcaa ctgtaacaag
    13981 cgcagtatca cgctcaacac caagaatgag aagggaaaag agattttccg caaactgata
    14041 aaggcttgcg acgtgatggt ggaaaacttt gctcctggcg cgctcgatcg tcaagggttt
    14101 acctgggaag tcattcgcga gctcaaccca cgcatgatct atgcttccgt gaaaggcttc
    14161 gggccaggcc cctacgaaga ttgcaaggtc tatgagaatg tcgcccaatg caccggcggc
    14221 gcggcgagca cgaccggctt cgacgatggc ccgccgctgg tgaccggagc gcagatcggt
    14281 gactcgggca ccggcatcca tctggttgcc gggatcctcg cggcgctttt ccagcgtgaa
    14341 aaaaccggta aaggacagcg ggtgacatgc gccatgcagg acaccgtgct caatctttgc
    14401 cgggtcaagc tgcgcgatca gcaacggctc gcggcaggac cgctgatgga atacccgcag
    14461 tatcccaacg gagtctttgg tgatgccgtg ccgcgcgcag ggaatgcgtc gggaggcgga
    14521 caaccaggct gggccgtgcg cactaaaggg ggcggaccga acgactatat ctacgtcatc
    14581 attcaaccgc ccggatggga acccttgatg aagctctgcg gccggcctga gctgatcacc
    14641 gatcccgaat gggcgactcc ggagaagcgc ctgccgaaac tgaagagatg ttttgagatc
    14701 atcgagcagt gggcgatgac tctcgataaa ttggaagtca tgcgcaagct caacgaattc
    14761 gacgtgccct gcgggccgat tctcgacatg cgcgaaattg ccgcggaaca gtcgctgcgc
    14821 gacaccggca ccgttgtcga agtggatcat cccaagcgcg gcaaatttct taccgtcggc
    14881 aatccgatca agctgtccga gtcgcggacg gaggtcagag cgtcgccgtt gttgggcgag
    14941 cacacggatg aaatcttgaa atcggtcgtc ggctggagtg aaacggaaat cgcggcagcg
    15001 cgcaaggaag gcgcaatttg atcttgctgt gcgcgtaggt gtgcgagcgc cacgaattca
    15061 gccggcagac atccgtcatc cgtcacgttc cgggttcaag ccctgagtga cgagttcaca
    15121 tgcctatgag caccgggagg ctttcttcac ttgcgccggc aggttccctt gcatgaacta
    15181 gcttagcttt tcagcaatgg cggaattctc gtacctgttc gcattccgat taatcgacct
    15241 aagcttcgga ggttccgatg aagaatgaaa atctgcgggc caccatcaac tctgctttcg
    15301 ctgcaggacg tcgagccttg agcgcgcccg aggcgcagca actttgcgag gcatacggaa
    15361 tctcgacgcc acggcaagcg ctggcaacga cggcgtcaga agcggcggag gtagcggcga
    15421 agttggggtt cccggttgta ctgaaaattg tctcggacga tatcctccac aaaaccgagg
    15481 cgggtggcgt gatcgtcggc ttggagaacg cagccgatgt tcgagcaggg ttcgaacggc
    15541 ttgtaagcaa tgccaagcag tacaagagca acgccagtat acagggtgtg caggtgcagc
    15601 aaatgctcaa aggcggtcag gaagtcatgg ttggcgccgt gaccgatgcc accttcggca
    15661 aaatgattgc cttcggtctt ggcggcattc tggttgaagt gatgaaagac atcacctttc
    15721 gcatggcgcc agtgagcgag accgaggctc tggcaatgct cgattctatc gcagccgctg
    15781 aagtgctgcg cggcgtgcgc ggcggtaaag gcgtcaatcg cgcggcgctg gccgatatca
    15841 tcgccaaagt atccaagctc gtgagtgatt ttcccgaaat caacgaggtg gatttgaatc
    15901 cgatctttgc cacggagacc ggggccactg ccgtggacgt gcggattgtt ctgggggagc
    15961 cgccaaaggc gcggcagcgt tacactcaga gtgaaattct cgcggctatg cagcggatca
    16021 tgaatcctcg ttcggtcgcg gtgatcggcg cctcggcgga agacggcaaa atcggcaact
    16081 ccgtgatgaa aaatcttatt aacggcggct atcaaggaac catcttgccg attcatccca
    16141 aggcgagtga aatcctcgga cgcaaggcct acccaagcgt gctcaaagtg ggcgaggaaa
    16201 tcgacgtcgc ggtgtttgcg attccggcca aattctgcgc cgccgcgatg gaggaggtcg
    16261 ggcaaaagaa aattcccggt gccgtgatga ttccgtccgg ctttgccgag atcggcgagc
    16321 aggcgctgca ggacgaacta atcggagtcg cgcgcaaaca caacgtgcgg gtcatgggcc
    16381 cgaatattta cggcttttac tatacgccgc aaaacctctg cgccactttt tgcacacctt
    16441 acgacgtcaa gggaaaagtc gctctgagct cgcagtcggg cggcgtcggc atgtcgatta
    16501 tcggattttc gcgctccacg aagatgggag tatcggccat cgtcggcctg ggcaacaagt
    16561 cggacctcga cgaagacgat ctcctgacct atttcgaaca ggatgacaac acccaagtca
    16621 tcgccatgca ttgtgaagat ttgaaagacg gccgttcgtt cgccgaggtg gcgagccggg
    16681 tatcaaaaac gaaacctgtg gtcgtgctca aagcagggcg gaccgcgctg ggcgcccgcg
    16741 ccgcggcgtc ccataccggg gcattggcgg gaaacgataa gatttatgat gacgtgctgc
    16801 ggcaatgcgg cgttgtcagg gcgaaaagtt taaacgacat gctgcaattc gctcgcggcc
    16861 tgcaggtatt gccgaatccc aaaggtgaaa atgtggtgat cattaccggc gccggcggat
    16921 cgggcgtgtt gttgtcggat gcctgcgtcg ataacggctt gtccctgatg aaaatgccgc
    16981 cggatctcga cgctgcgttt cgcaaattca ttccgccgtt cggcgccgcc ggcaatcccg
    17041 ttgacatcac cggcggcgag ccgccgacaa cctatgcgaa taccattcgc tacgcgctgg
    17101 gagaaccgcg catccactcg ctcatcctcg gatactggca tacgatcatc acgccgccga
    17161 tggtatttgc caaactgtgc gccgaagttg tccaggaggc gcgcgatcaa ggcatcgaca
    17221 agcctgtcgt cgcgtcgctg gtcggcgacg tgcaagtcga agaagcgagc gagtacctct
    17281 atgaacgcgg catcgtggct tatccgtata cgacggaatt acccgtcgca gtgctgggcg
    17341 ccaagtacaa gtgggcgcgg ggagcgggtg tgctctagca gcctgctgaa aaaaactcat
    17401 atccttcgtt gccctcaatc gcctccacgt caacgtgctc gctcagtacg tctccgctca
    17461 tcgatttgtc gcgcccctcg gctctgagat ctttttgagc agcctgcaat ggacagctgc
    17521 ttcatgcaaa atagggaggt gtatcatcag gcgggttgga tgttccttgc acgaagaacg
    17581 tcgaatgtcc gagagactcc ttcactttgc ttccagaatg atcccccggg cggccaagat
    17641 cggttttgcc gcttctcgca acccgggtaa tcgcctggcg aaaacgattc cggcgatggc
    17701 gcaaaagccg ccgccgatcg cgactaccat gggagcgccc acctggtcag cagcccatcc
    17761 tgccagcaag ctgccgaatg gcgccatgcc gaccagcatc atcgagaata ggctcatgac
    17821 ccggccgcgc atctgattat cggcgagggt ttgcagcacc gtattgctgg cggcgatcaa
    17881 gaccatccaa ccatagccaa cggcgacgag aagcagcagg gacagccaga atatcttggc
    17941 caacgtgaaa caaacgagtc ccattccgaa tcccagggtg gcgaagacga tcacccggcc
    18001 aagtccgata atgttttttc tttgcgcgag aaagagcgcg ccgatgagcg cgccaaaacc
    18061 caccgctccg aacatgatcc caagggtgcc tgcgccgctc ccatgcactt gcgccgcaaa
    18121 caccggcatc agtgtcatgt agggcatgcc catgacgccg accacgccca aaagcatgag
    18181 cagatcgcgt atcggcgtgg tcgccgtcgt atagcggtaa ccttcaatca acgagcgacc
    18241 gatcgaagtt accgtcgcat cgcgacatgg tggctcgatg aatttcatct tgagcaatgc
    18301 cgtaatggtc gctatgtaac tcgccgcatt gataacaaaa cagatgcctt cgccgaaccg
    18361 ggccaccacc agcccggcaa gcgcgggtcc gacgattcgc gcggcgttga tcatcgaaga
    18421 attgagcgcg accgcgttgg gcaagtcttc cgcatcgacc aatcgatgca ccaatgcctg
    18481 gcgcgcgggc atatcgaagg catagacgat tcccagcgcc atccccaaaa ggattatgtg
    18541 gactggctgt atccagccgg tcagtgccag caatgccagc agcagcgctt gagcgagtgc
    18601 cccgcattgc gtccataccg cgacccggtg cgtcgagtag cgatcgttga taacgccgcc
    18661 gataggaccc aaaaccaaga tcgggatttg cgacgcgaag ccaacgacac cgagcagggt
    18721 tgccgatccg gtgaggcgat acaccagcca gctcagcgcc acctgctgca tccaggtgcc
    18781 gcagaccgag accagctggc ctgtaagata gagccggtaa ttgcgatggc gcagcgagcg
    18841 ccatagcggc gatgaagcgg aaaaatttaa tcgcattgga gtgagtagga gcagactgga
    18901 acttagctgc attcgcgaaa ttgtctgatt atcccactca agggcgggaa gatgaagggc
    18961 ttacgagaag ttgagaattc gaatccacta aaaagttttt gcgctaacta tggatgtcct
    19021 tagcggacgc gctactccgg ggatccgtac acggctaatc ttcgatgatt tcttttgtca
    19081 gattgtcgac gacaaccggg gtctgaaagt agacaatggc cggttcggcg ccatattttt
    19141 ctttcacgaa gctcttccat tcctttgtat agaggctctc ggcatcttct cttgattgcc
    19201 ataagtacac cccaccggcc gtgccgccat cttcggagag aagatagtac ttgcgaatca
    19261 acccccgtgc agcctgatac ttcggcgcgg tgctcgtaaa tatctctttg gcttgctccc
    19321 ggctcatagg cgtcggtatt ttgaattgaa cgacggctgt caccataggt cctccatctt
    19381 aaacttcgac gatcagccaa tcatatcgcc gagccaatct cgaacggtat ttgagtcttc
    19441 ttcgaaatat tcagcaaatc atccacgacg ggtttcaaca gcggaatgcc ttctttgctg
    19501 cgaatcgcct cggcttctct ctcgggatcg cccgggatca acgggccact ggtgcccgga
    19561 gcgggtttgg ttttgcgaaa gacgcgaatc cattcatcga tctgcttttg aattggtctt
    19621 tatcgatgaa gccgtcgatc tgcatggcgc cgaaaaagtg cccgatgcct ttcccggtct
    19681 gttaacgtaa aggtatctga gcagtgccgc attgccaagc ttgttttgac tcagctttcc
    19741 gaagggccta tgattctgtc gaacggagga tacatgaaac taaaggttgt cttagaacct
    19801 tccgacgaag gcggttttat tgtttatgtt ccttcccttc ccggatgcgt gtccgaaggg
    19861 gaaagcgaaa ccgaggcgct tgagaacatc cgtgaggcgg ttgagctata tcttgagccc
    19921 gtggacgatg accttgccgg agtcggaaaa gaggttcggg aaatcgtttt gtgagcgagt
    19981 tgccgagcct atcttacgac cggatgtgaa agcgcttcaa tgcg
//
LOCUS       DASUVS010000489        10908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_215587, whole genome shotgun sequence.
ACCESSION   DASUVS010000489 DASUVS010000000
VERSION     DASUVS010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10908
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215587"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..683)
                     /locus_tag="VF182_25570"
     CDS             complement(249..683)
                     /locus_tag="VF182_25570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6440514.1"
                     /translation="MKPRTIVKKISSEKEWQQALRIRVRVFVREQGVPAELELDDDDR
                     KALHLLAFIGTRAIGTARIVMRPRQAKIGRMAVLKSHRRKKVGRKLLARAIATAKKQG
                     ARKIYLHAQVPVVEFYESAGFRCVGPVFEEAGIAHRKMVLKD"
     gene            864..1982
                     /locus_tag="VF182_25575"
     CDS             864..1982
                     /locus_tag="VF182_25575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440515.1"
                     /translation="MGFDSGAADRDSILQRGLCYVLFAYDAARSIDLDAAERRIHEAT
                     QRQTIQHKRRAPSYLEYQPPPLRLSQEIQPIKIGEYTSHRMVDLVLYDFGAVSVIYSI
                     PIEGPFAALLALSEDLYDNEILLTESRMRVEQIIGALGDAAINVHSASLVEDYVIFRI
                     TALEPATSPAAFCANHAPDIARILRAERQRLSVEEVEDALSARTSFGTDDLTIIDWNA
                     ALLVDREGDDVQAVLEFANVELLEMRYLDQKLDRALFEAYETLSKRSWSLPKFLGYYS
                     TDLRRVAELQVDNALLFEGVNNTLKLVGDQYLARVYRLVNRRLHLADWDSSIIRKLQT
                     LESIYEKIADQAATRRLEILEWVIIFLIAFSILLEFIH"
     gene            2654..2992
                     /locus_tag="VF182_25580"
     CDS             2654..2992
                     /locus_tag="VF182_25580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440516.1"
                     /translation="MRDYADIGAGAALALAAFLALGLILHWFFFVCALPAFGLFVFTE
                     WATARQQARSPGYRYNFDEDGNIEAIQCENCGRASRSQHDIEAKFCSFCGRYHKRHEN
                     SKKTAGNFPS"
     gene            3079..3564
                     /locus_tag="VF182_25585"
     CDS             3079..3564
                     /locus_tag="VF182_25585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440517.1"
                     /translation="MTSCKIIPFLVLWGALSAIATPALASEVPANYSKLSNKELKSRA
                     LTVVKAIRQLVNSYNEKDRDLTSDFEAEYLATRTTERPLLQDQWRKKIDAAQGASLGN
                     YRQNFAPNAALLREELQRRLPKRLQSRDMPKLYSTPANVLSLEVVADDLELLAKSLPE
                     T"
     gene            4590..4760
                     /locus_tag="VF182_25590"
     CDS             4590..4760
                     /locus_tag="VF182_25590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440518.1"
                     /translation="MGPQFYTLLALTDTIVGELRWGMKKKRRSAKVADPLIAEPRQLR
                     KRLSGKLKIERL"
     gene            complement(5742..6755)
                     /locus_tag="VF182_25595"
     CDS             complement(5742..6755)
                     /locus_tag="VF182_25595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440519.1"
                     /translation="MRLFLRKVLLFMLVQCLIWTGVVALYIRSEATKGDGFAKGYLGA
                     TIDKHNRLAQQPSPRIVFVGGSNLAFGLDSAAVERSLGYHPVNMGLDISLGLDFMLRE
                     IEPFLARGDVVVISPEYEEFIDMYPGKSITLFPEATIYPRAMRFFSYRHFTLLLDEGL
                     ILIGEITRAAVRFLTVGVSNEVRSDNPYVRSAFNEYGDITRHHNLKSRHISIRQFGPS
                     SPESITRVIDRLNQFNDHCRSDGISVFYSYPPIFSGQLEAYTEMIHDIASNLTRRLHF
                     PILNTPDDMSFPANYMFDHEYHLTLSGKQIRTNQLITTLRKKLTAPENTLDLTEPGIL
                     SAH"
     gene            complement(6798..8261)
                     /locus_tag="VF182_25600"
     CDS             complement(6798..8261)
                     /locus_tag="VF182_25600"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012162674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEX6440520.1"
                     /translation="MTFTSLTFVFFLGLVFISYWSLTNRFHQNVLLLCVSYIFYGWWD
                     YRFCILMFGTSILDYGIGLLIYRTAELRWRRFLLILNIACNLGVLGFFKYFNFFADNF
                     QSLAASLGWQVHPVTLRILLPAGISFYTFQAMSYTIDIYRGKMVPTRHPIEYLAFISF
                     FPHLVAGPIMRAAHLLPQFLRRRQFDYSRSVDGCRQILWGFFKKMVLADNLAVIVDKI
                     YADPASMTGPQLAAATVCFAFQIYYDFSAYSDIAIGTAKLFGFDLMRNFAYPYFSQTM
                     SEFWRRWHISLSTWFRDYVYISMGGGRVSRLRRACNVLVTFLLSGFWHGAAWNFALWG
                     GLNGITLIPEQLQSTKAALKAVDTPGGERRLPTLVTLLRIFRTFALTCALWIFFRARS
                     LQEALSIFRKIAVDAPHLSTYGSVNVVRGISPIGGKVFLMAAALICLEWWQRAHPHPL
                     VLSRWPGSARWIAYTGLFWAVIYWGTHSPVQFIYFQF"
     gene            8683..8777
                     /locus_tag="VF182_25605"
     tRNA            8683..8777
                     /locus_tag="VF182_25605"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8717..8719,aa:Sec,seq:tca)
     gene            complement(8798..10447)
                     /gene="pgm"
                     /locus_tag="VF182_25610"
     CDS             complement(8798..10447)
                     /gene="pgm"
                     /locus_tag="VF182_25610"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007950682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HEX6440521.1"
                     /translation="MSIHPLAGKPAPKEVLINVGELETAYYDRIPDIDNPLQRVGFGT
                     SGHRGSPLESSLNEAHILAMTQAICDYRALQKITGPLFMGKDTHAVSGPAQRTAIEVL
                     AANRIETVIQQDDGFTPTPSISRAILNHNRNKKNTLADGIVVTPSHNPPADGGFKYNP
                     PNGGPADTDVTDWIQQRANDLLKNGNREVKRLSYESALRATNIHKQDLLTPYVDDLLE
                     VIDMEAISAGLVKIGIDPLGGAAVSYWQAIGERYRLDLTVVNTQIDPTFKFMTLDHDG
                     KIRMDCSSPYAMASLVNLKDQYQIAMGNDADADRHGIVTPGVGLMNPNHYLSAAIDYL
                     LVDRSDWNPRAAVGKTLVSSSLIDRVVELRGRKLFEAPVGFKWFSQGLLDGTICFGGE
                     ESAGASFLCRDGSVWSTDKDGIILCLLAAEIIARTGKDPAQRYEEMVRDLGRPYYART
                     DVPATAAQKAALKKLSPEALSARKLAGEIITAKLTRASGNNAEIGGLKVASQNGWFAV
                     RPSGTEDLCKIYAESMRSEEHLKQIQDEAWQIMQAVFKASA"
BASE COUNT         2651 a         2880 c         2815 g         2562 t
ORIGIN      
        1 tgctccttta cttcaggttc tgctgatttg attgcatcgc gcgcgaccac tcatgcggcg
       61 cgagtcgaga taacaggcgc cagcacaaga tcgcccagtg aagactctgc tacttactcc
      121 gccgccaaat gcgttcgcgc ataaagcgat ctgctttact agtcaccggc tcttaatgta
      181 gcgcatgaaa agcgcgctta gataatcgga ctcgtattcg caacttctgt aaggataacc
      241 ttgcctgttt aatctttcag aaccattttt cgatgagcaa tgccggcttc ctcgaaaacc
      301 ggaccgacgc agcggaatcc cgcggattcg tagaattcga ctaccggcac ctgggcgtga
      361 agatagattt tccgcgcgcc ctgctttttt gcggtcgcaa tcgcgcgggc caataatttc
      421 ctgccaacct tttttcgccg gtgacttttg agaaccgcca tgcggccgat tttggcttgg
      481 cgcgggcgca tcactatccg agctgtgcca atcgctctcg ttccaataaa ggcgagcaga
      541 tgaagggctt ttctatcgtc gtcgtccaac tcaagctctg cgggcacgcc ctgctcccgg
      601 acaaacacgc ggacgcgaat tctcaaggcc tgctgccatt ctttttcaga agaaattttt
      661 tttactatag tccgtggttt cattgtctgc gcatgacatc gatcggctgc ggtggatgag
      721 accaattgtt ctgaaatccg cgaggtatag cgactaagct ctcatatcga atggaacatg
      781 tacgggttct cttcttaccg gtcgggccta ggccaggtaa acttgcggtg ctatgcggtg
      841 ccgcttcgtg ctagattaga cacatgggct tcgattccgg cgcggcagac agggattcga
      901 tacttcaaag gggcctctgc tatgtgctct tcgcctacga tgcggcgcgc tccatcgacc
      961 ttgatgccgc cgaacgtcgc attcatgaag cgacgcagcg ccagacgatc cagcacaaac
     1021 ggcgcgctcc aagctatctc gaataccagc cgccaccgct gagattaagc caggagatcc
     1081 agccgatcaa gatcggcgaa tataccagcc atcgaatggt ggacctggtt ctctacgact
     1141 tcggcgccgt ctccgtcatc tatagtattc cgattgaggg gccatttgcg gccttgctgg
     1201 cgctcagcga agatctttac gacaacgaga ttttgctgac cgagtcgcgc atgcgggtgg
     1261 aacagatcat cggcgcgctc ggtgatgcgg ccataaatgt gcatagcgcc agccttgtgg
     1321 aagattacgt catctttcgg atcactgctc tggagccggc cacgagtcca gcggcttttt
     1381 gcgccaacca tgcgcccgat attgcaagaa tcctgcgcgc cgaacgccag cgtctttcgg
     1441 tcgaggaagt cgaagacgcg ctgagcgctc ggacttcttt tggcaccgac gacctaacga
     1501 ttatcgattg gaacgccgcg ctgctggtcg accgcgaagg cgacgacgtt caggcggttt
     1561 tagagtttgc caatgtagag ttgttggaaa tgcgctacct cgatcagaaa ctcgatcgcg
     1621 cattgtttga agcctacgaa acactctcta agcggtcgtg gagcttgccg aagtttctcg
     1681 gctattacag cactgatttg cgccgtgtgg ccgagcttca agttgataat gcgctcttat
     1741 tcgaaggcgt caacaacacg cttaagcttg tcggcgatca atatttggcg cgcgtctacc
     1801 ggttggtgaa tcggagattg cacctcgctg attgggattc gagcatcatt cgcaagctgc
     1861 aaacgctcga aagcatctat gaaaaaatcg ccgatcaggc cgccactcgc cgtctggaaa
     1921 ttcttgagtg ggtgatcatt tttctgatcg ctttttccat tttgctggag tttatccact
     1981 gagcggattg caccggcgcc ggaacaagat gtaaggtgtt gccgatcgcg gaacttctag
     2041 cgcttcctta tgttgagcaa agtctacaac ccgagtcttc gtgattgcgc gcgatgaatt
     2101 tcatttttcc ttttggcgcc atgcggcaag atcgtcgagc cccgcacctc tacttctcgg
     2161 gtgcgttttg cgctaagttc aatttagctg tctagaaatg cgcccgatca atcacaagaa
     2221 tcatcgaata accgttcacc caaaatggga tcccgtaagc cgatcctggg cctgcgacgc
     2281 cacagtttac atacctcaag caggtcgatc agaagctgtt cctttttttg acccggtgcg
     2341 ccattataag atcaaagcgt cggcggtaac tgccattcta aacgcgatta gatcttggat
     2401 cgaccacggc aaacccgaac agttcgcggt gcaataagcg aaggcggcgg ccgcgctccg
     2461 gacctggcgt tcgccctgtc atttttccct gtaccttgta tcgggcacgg cacggcggat
     2521 gggtgacttc aaaatcaccc atcgatgtta aaaattcgtc aaaacgcgct agactagatg
     2581 taggttggcc gcgggttacc gacggatgga ttgatagcga gccgaacctc tttagtgaga
     2641 tggcgtagct aacgtgagag actatgctga catcggggcg ggagcagctt tagcgctggc
     2701 ggcgttcctg gcgcttgggc tgatccttca ctggttcttt tttgtctgcg ctcttccggc
     2761 gtttggtctt ttcgttttta ctgaatgggc aaccgctcgg caacaggcgc gcagcccggg
     2821 gtatcgttac aacttcgatg aggacggcaa catcgaagcc attcagtgtg aaaactgcgg
     2881 gagagcaagt cgcagccaac atgatatcga ggcgaaattc tgcagtttct gcggccgtta
     2941 tcacaagcga catgagaact cgaaaaaaac cgcgggcaat tttccatcat gacgcttcgg
     3001 ccgacattta gtaaccctgc tttcatcgtc agagttgtgc gggccggctc cgctataata
     3061 atgacttcgt aggttgttgt gaccagttgt aagatcattc catttctggt tctttggggc
     3121 gcgctgagcg cgatagccac accggcttta gcttccgagg ttccagcgaa ctattccaaa
     3181 ctctcgaaca aagagcttaa gagccgagct ttgaccgtag tcaaagccat taggcaactg
     3241 gttaattcgt ataatgaaaa agatcgtgat ctgactagtg attttgaagc ggagtacttg
     3301 gcgacccgaa ccaccgaacg accattgctg caagatcaat ggcgcaaaaa gattgacgct
     3361 gcgcagggtg cctcgctcgg gaattaccgg caaaactttg cgcccaacgc tgcactgtta
     3421 agagaggaac tgcagaggag actgcccaaa cgcctgcaaa gccgagacat gccgaagctg
     3481 tattcaactc cggcgaatgt gctatccctt gaagtagtcg ccgatgactt ggagctactt
     3541 gctaaatcac ttcctgagac ttgatcatca accggaatga gcccacatcc gattagctaa
     3601 cttgaccttc ttacctgccg acgtggaccg gcggctttca ccaacgttga ccggataaaa
     3661 taagggtcaa cttctatgag ctgacaagcc cataagaagc taccgacttc ctcgttggaa
     3721 gacgcaagcc aatgcctagc ataacgtctg acggctggag ctttcgataa agctggctcc
     3781 tccgagtccc ggatcgcttg aatgataacc tgagtccata gctgtgcttc aggcaacata
     3841 atcaactcca ttaaaagatt tagccgtcga aatggctgag aatagacatt cgcttcttta
     3901 ggcatacacg aggcgtcata gcgggatcgc cacaccagaa ccaatcatcc aagggcatct
     3961 cgacatcgat agcagggcga ttattcgctg gcatttcgag caaaccgtat acgaacctat
     4021 ccacgtcagc caataatcca ctgatgggag agatttgctg agattgcaca atctcagccc
     4081 ggtttgacct tatgcccgga gtattccggc gtaatgaagc ccatttcgct cgggccagca
     4141 aggaatcctc cggcaatctt ggttctgaat acgagccgcc ttctagcggt cggagcccat
     4201 tctggcgcca ataagtgatg ggttggaatt cagtgacttc agtcattcga tccctctctt
     4261 ccctgaatgg atgtcagtgg cgaattacgg gttcattcag caaccaggag accagggacg
     4321 tgtgggaaca tggaatcagt tgcgagaatt cttgagaagc ctgatagtcc cgcttccagg
     4381 aatgcttcaa tcgcggctgt cgcgtaaacg aaacagacca gtagagctct gggccgagta
     4441 tttgaaacga gacgatctca tgcccgagat cattttgccg cgtctatgcc aacgttctat
     4501 ggcaccatac ttcgtaattt tacttgcgat tgagatgagg cctgaactat tattatccca
     4561 tggtcaaaaa actgaagaag cccatatcta tgggaccgca gttctatacg cttctcgcac
     4621 ttacggacac tattgttggt gagctgcgtt gggggatgaa gaaaaaacgc cgatcggcaa
     4681 aggtagccga tccgttgatt gcagaaccgc gacaactaag aaaacgtttg agcggcaagc
     4741 tgaagataga gcgcctttga tcagtctacc gtgagtgaaa aagccaaacc gtctagatat
     4801 gtgagccgag accgacaagt agtcgctgag acagaactgc ataatatata gcaccaacaa
     4861 caacccacct atcagcatct actgcaaaca acgatagcct atatcggccg cccgcaactc
     4921 acgtgagaat ggcgagccgc gtttgatatt tttgaaaaac caccctgcag gctacataac
     4981 acggtagccc acctgcctta ctcgccgacg atcaggattt tcttggcgac gacgcttcct
     5041 cgtggaacca ctcgcattta ccgtcacgat gcttctgaat acaactattg caaaagctat
     5101 gcggcggatc ggtactctct cgttgctcgt agcccgatct cggccagtgc tcacagtttc
     5161 tgcaaaagtg ccatacgttt tcgctgcgca actttcggta aaaagttttt gtcacgggat
     5221 cacctcgaag tccatcacga tgtgtcgatc tcgcagggtt taaagaactc acgggccgag
     5281 tggttctgaa tttcgctaca ttgcgggaaa acgaactcgt acagctagat accagaggat
     5341 ttatatcagc cagccccctt accatgtttt cgccacctcg ccgacaatta ccgtgtaaaa
     5401 acgcgggggt catacacctt aggatacggc tgtgtcaacc ggaccggcgc tccgaagaac
     5461 aatataacag ggggaccgtc tttgaagttt aaatctagag agctgttaca ttctggcggt
     5521 tattcaatat cctcaagggt ttgtagccat atgagcgctt caagcttttg cactccccag
     5581 gaccaagagt cccctttttg ggtgtttggc agggttcaac cgtcgctcca gaggtcgagc
     5641 ggatctggcc gatattaagg atcttgtaag atttactgga tatgactgtt cgcggcggac
     5701 ggctgcaact ccgtgtcctg gtactttgct tgagccgata cttaatgcgc gctcagaatg
     5761 ccgggttcgg taagatctaa agtattctca ggagccgtca acttttttcg gagcgtcgta
     5821 ataagctgat tggtgcggat ttgcttaccc gatagagtta ggtggtactc gtgatcgaac
     5881 atgtagtttg ccgggaagct catgtcgtca ggagtattca ggataggaaa atggagccga
     5941 cgcgtgagat tcgaagcgat gtcatgaatc atctctgtgt aagcttctaa ctgccctgaa
     6001 aaaatcggcg ggtaggaata aaagaccgaa atcccatcgc ttcgacagtg gtcgttgaat
     6061 tggttgaggc gatcgatcac tcgcgtgata ctctcaggag aggacgggcc aaattgtcta
     6121 attgaaatat gtcgcgattt taaattatgg tgccgcgtga tatcgccata ctcattgaac
     6181 gcgctacgga cgtatgggtt gtctgatcga acctcgttgc ttacccctac agttaaaaac
     6241 cgcacggctg ctctcgtgat ttcaccgatc aatatcaaac cctcatctag aagcagcgtg
     6301 aaatgcctgt aactgaaaaa gcgcatcgcc cgcggataaa ttgtcgcttc gggaaacaaa
     6361 gtgatcgact ttcccggata catgtcgatg aactcttcgt attccggaga gataaccacc
     6421 acatcgccgc gcgccaaaaa tggctcgatc tctcttaaca tgaagtccag accgaggctt
     6481 atgtccaatc ccatattcac cggatgataa cctaaggacc tttctactgc cgcggagtcg
     6541 aggccgaacg ccagattgga cccgccgaca aacacaatcc gaggagacgg ttgttgtgca
     6601 agccggttat gcttatcgat cgtagctccg aggtatcctt ttgcaaagcc gtcgcctttt
     6661 gttgcctccg atctaatgta cagtgctact actcccgtcc agattaagca ttggacgagc
     6721 atgaagagca acactttacg cagaaataat cgcatacacg gggcgctata tttattgata
     6781 ggacaacacc tcagcaatca aaattgaaag tagatgaact gaaccggact atgtgtcccc
     6841 cagtaaatga ctgcccagaa caggccggta taggcgatcc atcgggcgga ccccggccac
     6901 ctacttagca ccagcggatg tggatgcgct cgttgccacc actccaagca gattagagca
     6961 gccgccatca gaaacacttt gccgccgatg ggagaaatac ctcgtacaac attgacagag
     7021 ccgtaggttg aaaggtgtgg cgcatccacg gctatttttc tgaagattga aagggcctct
     7081 tgaaggctac gggcgcgaaa aaaaatccat agcgcacatg tcagcgcaaa agttctaaag
     7141 atccgtaaca gcgtcaccaa cgttggcagt cgacgctctc cgccgggggt atcgacggcc
     7201 ttaagagcag ccttggtcga ttgcaactgt tccggaatca gagtgatacc gttgagcccg
     7261 ccccatagag caaaattcca ggctgcccca tgccagaatc cactcagcaa aaaggtgact
     7321 agaacattgc acgctcggcg cagacgggag acacgacctc ctcccattga tatgtagacg
     7381 taatcgcgga accaagttga gagcgagata tgccaccttc gccagaactc ggacatcgtt
     7441 tgcgagaagt acgggtaagc aaaattacgc ataagatcga agccaaaaag cttagccgtc
     7501 ccaatggcaa tgtcggaata ggcggagaaa tcataataaa tctggaacgc aaaacagacg
     7561 gttgcggccg caagttgcgg ccctgtcatc gacgccgggt cagcatatat tttgtcgacg
     7621 attacggcga gattgtccgc caacaccatt ttcttaaaaa aaccccagag aatctgtcgg
     7681 cagccgtcca cggaccgcga ataatcgaat tgtctccgcc tgagaaactg tggcagcaga
     7741 tgtgccgcgc gcataatcgg acctgcgaca agatgcggga aaaatgagat gaacgccaag
     7801 tattcgatag gatggcgagt gggaaccatc ttcccccgat aaatgtcgat ggtgtagctc
     7861 atcgcctgaa aagtatagaa gctgattcca gccggcagca gaatccgcag cgtcacagga
     7921 tgcacctgcc accccaagga cgctgcaagg ctttgaaaat tatctgcgaa gaaattgaaa
     7981 tacttgaaaa agccaaggac accgagattg caagcgatat tcagaatcag taagaagcgg
     8041 cgccaccgaa gctctgctgt tcgatatatc aataagccga tgccgtagtc caaaatactg
     8101 gtgccaaaca tgaggatgca aaatcggtaa tcccaccacc cgtaaaagat gtaactgacg
     8161 cacagcaaca gcacattctg gtgaaagcgg ttggtaagac tccagtatga aataaatacg
     8221 agaccgagga agaaaacaaa ggtgaggcta gtaaacgtca tagcagcctt ttagctcatg
     8281 cacgtagtca gaaacctgca tgtcacaccc ggtgtatgtg gcgcaggtat atatccctgg
     8341 acaagttgca agtcaaacga gcgcgatcgg actttcccga cagtcccaga ctgcttcttt
     8401 tttgttgatc aggcgaggag atgtcggggc gcgatgggtt cccggcctga gttgagccgc
     8461 gtgttcacgc gccgttagcc ggccgtgcct acaggctcga gtcagcggaa gaagtaagga
     8521 gggaggatca cgaccggaag cgagggtcaa tgcgtatcga taacgatgtc tgataagtca
     8581 aagctgacta gtgaaaagtt cggcttgatt gggacgtaaa gcgcgtcgcc atagccagct
     8641 ttaccgggac tgtaggacat gctaaccaaa tgaatttgcg atggagaagg aaggtgcccg
     8701 gtggtgccgc tggtcttcaa aaccagtctg ggggccgtaa ggttcccgga gggttcgact
     8761 ccctcccttc tccgccagat aaatagattg cccttcatca tgcgctcgct ttgaaaaccg
     8821 cttgcatgat ctgccaggct tcgtcttgaa tttgcttgag atgttcctca ctgcgcatgc
     8881 tttctgcgta gattttgcac aaatcctcag ttccggacgg gcgcacggca aaccagccgt
     8941 tttgtgaagc gactttgagc ccgccgattt cagcattgtt tcccgacgct cgcgtcagct
     9001 tggcagtgat gatttcgcca gccagtttcc gagccgacaa agcctcagga ctgagctttt
     9061 tgagcgctgc tttctgtgcc gccgtggcgg ggacatcggt gcgcgcatag taaggtctac
     9121 cgagatctcg aaccatctct tcataccgtt gggcgggatc ttttccggtg cgggcgatga
     9181 tctccgccgc caataggcag agaataattc catccttatc ggtgctccag accgacccgt
     9241 cgcggcatag aaaactggcc ccggcgcttt cctcgccgcc aaagcaaatc gtgccgtcaa
     9301 gcaacccttg tgaaaaccat ttaaaaccca ccggcgcctc gaaaagcttg cgcccgcgta
     9361 actccacgac ccgatcgatc agcgaactgc tgaccagagt tttgccgacc gccgcccgag
     9421 gattccagtc ggaccgatcg accagcaggt aatcaatcgc tgccgacagg tagtgattcg
     9481 gattcattag ccccacgccc ggggtgacaa tgccgtgacg atcggcgtcg gcatcattac
     9541 ccatcgctat ctgatattga tcttttaggt tcaccaggct cgccatggcg taagggctcg
     9601 aacaatccat ccggatttta ccgtcgtgat caagcgtcat gaacttaaaa gtaggatcaa
     9661 tttgggtgtt gaccacagtc agatccaacc gatatcgctc gccgatggct tgccagtagg
     9721 aaaccgccgc gccgccaagc gggtcaatac cgattttcac gagaccggcg ctgattgctt
     9781 ccatgtcgat gacttcgagc agatcatcga catacggcgt cagcaaatcc tgtttatgaa
     9841 tattggttgc ccgcagtgcg ctttcatagg acagccgttt cacttcccga ttgccattct
     9901 ttaacagatc gttggcccgt tgctgaatcc agtcggtcac gtcagtgtcg gcgggaccgc
     9961 cgttaggcgg attgtattta aagccgccat ccgccggcgg gttatgcgac ggtgtgacga
    10021 cgataccgtc ggcaagagta tttttcttgt tgcgattgtg attcagaatc gctcgggaaa
    10081 tcgatggcgt cggcgtaaag ccatcgtcct gctggatcac ggtttcgatt ctattggcgg
    10141 cgagaacttc aatggcggtg cgctgcgccg ggccggaaac cgcgtgagtg tccttgccca
    10201 taaacagcgg tccggttatt ttctgcaagg cgcgataatc acagattgcc tgggtcatcg
    10261 ccaggatatg cgcctcatta agcgagcttt ccagcgggct cccgcgatga ccgctggtgc
    10321 cgaagccgac tctctgcaac ggattatcaa tgtcaggaat tcgatcgtag taggcggtct
    10381 cgagttcgcc gacattgatc aagacttctt tgggcgcggg tttccccgcc aaaggatgta
    10441 tgctcatgag tctgctcctt ttgacgccgg atgggttggc accccgttac gaacaatcta
    10501 atctaaactt ttcgcggcgt gtgctccaga caggttgata ttttttatcc gggaagccgc
    10561 ccgcccgcca gcgttaacct gcgtctgttg ccgcaaaaac ctatggtctc acaactcgat
    10621 tcaaggctcg cgacacagaa gccttttcgg tcagcggaac ggcccgtttc cagtgcgcaa
    10681 tcaaagcgga gcgcagccgt cctcggcaac ttggactcgt gccggcaaca acccagcata
    10741 gcttccaact attcgcgcag gttaaaaaat gttcgacgag ctcgggccgg cattattttt
    10801 tttcgatctt ttgcaggttt accctcgaac cttatctaga ctcggcgtta tgattccgct
    10861 acagtccgaa gcgtcgggcc gcctaggagc agaatcatgg acacgctg
//
LOCUS       DASUVS010000490         6424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_1089487, whole genome shotgun sequence.
ACCESSION   DASUVS010000490 DASUVS010000000
VERSION     DASUVS010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6424
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089487"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..96)
                     /locus_tag="VF182_25615"
     CDS             complement(<1..96)
                     /locus_tag="VF182_25615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX6440522.1"
                     /translation="MRYGFVIDQNRCIGCHACTVACKEEHNIAVGV"
     gene            complement(299..1165)
                     /locus_tag="VF182_25620"
     CDS             complement(299..1165)
                     /locus_tag="VF182_25620"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6440523.1"
                     /translation="MLPIFFGILTSVCFAIASLLAQRGYHLAAAPWGAWITIAGNTVF
                     LLSAHFLLDPFAPILVNDNLIFVAVGLLVPGVTRVLSFRGIRTMGSSVTSTIINTTPM
                     FSTVLAILLLGERPGPWVIAGVFVTVCGLITVSWTGATSSYHKIELIFPFLCALIFAW
                     KDVTVRWGLGDGGGQPILAAGIAALTSTVEIFLITRYLHGEKFRLPAANAARWFVWSG
                     IFTGGSFLCMYVALSMERVTIIAPLINSYAVFVLMLTPFMARQIESVTRRKIAGAAMV
                     VAGIFMVSLGKH"
     gene            1243..2307
                     /gene="mutY"
                     /locus_tag="VF182_25625"
     CDS             1243..2307
                     /gene="mutY"
                     /locus_tag="VF182_25625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX6440524.1"
                     /translation="MLPTNLALLRRRLLGWYEKNRRDLPWRRTSDPYAIWISETMLQQ
                     TQVKTVVPYYERFLKAFPTIAALDHAPHRQVLRAWSGLGYYRRAENLKKSASELMRRH
                     GGMMPQDYESLRALPGIGDYTAGAILSIAFQKAYPAVDGNARRVLGRLFNLAGAKQVR
                     AAAEKLTPKSNPGNFNQAVMELGATICLHAEPLCSVCPLVKHCRLHSGGRSSKKLASR
                     KKLKVRNVTWPMAIIRRGEKILLRRRGKTGLLAGMWELPGGEESSNHGVLRTLSRHMQ
                     GIGPVLPIPLSIGEIRHSITNRRIRSPIFLFDLAVNEPIGLPGSGWRWVTPAALDRYP
                     ASSMTLKAARVLASYEKSAS"
     gene            2288..3046
                     /gene="mtgA"
                     /locus_tag="VF182_25630"
     CDS             2288..3046
                     /gene="mtgA"
                     /locus_tag="VF182_25630"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX6440525.1"
                     /translation="MKRVLLKSLLLSAIIVSLVHFYLALPNVSLLKTRNPRSSALMEL
                     RDQENREKKLRVSRQQIWVPYGRISNHLKKAVLLSEDASFFSHNGVDLDELKESLKRD
                     LTTFSFKRGGSTITMQLARNLFLNPAKNPLRKIKEIIIAWQLEQALSKIRIFEIYLNV
                     VEWGRNIYGAEAGSRHYFGKAAATLDPLEAATLVALLPNPRTTREKSILQRRNLVLAR
                     LAEVGHISKEEYDRVKQIPLFHTVKQEAPGLLQD"
     gene            3203..3409
                     /locus_tag="VF182_25635"
     CDS             3203..3409
                     /locus_tag="VF182_25635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440526.1"
                     /translation="MEKGTKKLDTTLGELIAAASEVAFEYSDNDRDAYKLAQAALIEI
                     LRKASHAGSLDDFGEPATKKTYLH"
     gene            3753..4718
                     /gene="msrP"
                     /locus_tag="VF182_25640"
     CDS             3753..4718
                     /gene="msrP"
                     /locus_tag="VF182_25640"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEX6440527.1"
                     /translation="MLIRRPEDIKSSEITPKELYFNRRQFMLAASGAAAAAGAALAGF
                     DVFRRSGHALAAEQIANVKKSSYSTSEKQNSFKDITNYNNFYELGPDKEDPAANAKYF
                     TPTRPWTIAVEGEVKNPKTYDIDALTKLSPLEERVYRLRCVEAWSMVIPWIGFPLSGL
                     IKLAEPTGNAKFIQFVTLHDPKRMPGQKLPIMEWPYVEGLRMDEAMHPLTILSVGLYG
                     QVLPAPNGAPIRVVVPWKYGFKSIKSIVKIRFVEKQPPASWNMMQPQEYGFYSNVNPE
                     VDHPRWTQATERRIGEFLRRKTLMFNGYGDQVAQLYSGMDLKKLY"
     gene            4835..5482
                     /locus_tag="VF182_25645"
     CDS             4835..5482
                     /locus_tag="VF182_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HEX6440528.1"
                     /translation="MRSWIKPALFTACLIPLAQLAYGFYTDDLTANPIEYITRFTGSW
                     ALILLITSLAITPLRKIFGWNLIRYRRMLGLFAFFYALLHFSTYIVLDHFFDFQAIAK
                     DIYKRPYVTAGFTAFVLMVPLALTSTAAMIRRLGKRWQQLHRLAYVAAAAGVLHFYWL
                     VKSDIRRPVQYGAVLALLLAVRLAYALLPMPSKRITGKAKIIAPQTDRSRLAGRP"
     gene            complement(5581..>6424)
                     /locus_tag="VF182_25650"
     CDS             complement(5581..>6424)
                     /locus_tag="VF182_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX6440529.1"
                     /translation="DDLMQTDSQGRGIINVLVADQLINAPKLYATFLLWLLSELFERL
                     PEVGDPEKPKLIFFFDEAHLLFNDAPAVLLEKIEQVIRLIRSKGIGVYFVTQNPLDVP
                     DVVLGQLGHRVQHALRAFTPRDQKAVKAAAQTFRQNSAIDVEAAITELAVGEALVSFL
                     DEKGRPNIVDRALIYPPQSQLPPLSASERETVVKRSTIFGHYEKMVDRESAYEMLKAR
                     APLPEEPPAKTASGSWQSEAGGLFDAMGKSAARAIGSQVGREIIRGVLGSLFGAGATR
                     KRRY"
BASE COUNT         1570 a         1780 c         1658 g         1416 t
ORIGIN      
        1 cactccgacg gcaatattgt gttcttcctt gcatgccacc gtgcaggcat gacacccgat
       61 acaacggttc tgatcgatga caaatccgta gcgcattgag aacctttcgt cttaatagtg
      121 ccaagcagcg aaaataattc ttaggtcata gcaaaggcgg cgcgaaactc caaccatggt
      181 tccgtccaaa ggatgaatta tgaaggatga gatctgaaac aaactcgttc cggaagcctc
      241 attttcagct ttccgctttc atcctttcat ctttgcagag ggttttctcg acagcctgct
      301 aatgttttcc caatgagacc atgaagattc cggccaccac catagcggcg ccggcaatct
      361 tgcgccgggt gacgctctct atctgtcgcg ccatgaaagg cgtcaacatc agcacaaaga
      421 cggcgtaact attgatcagc ggagcgataa tcgtgacccg ctccatactg agggcaacat
      481 acatacatag gaacgacccg ccggtaaaaa tccccgacca gacaaaccag cgcgcggcat
      541 tggcggcagg caggcggaat ttttcgccgt gaagatatcg ggtgattaga aaaatttcga
      601 ccgtcgaggt gagcgcggcg atccccgccg ccaggatcgg ctgtccgccg ccgtcgccga
      661 ggccccaacg gaccgtgacg tccttccatg cgaagatcaa cgcgcacagg aagggaaaga
      721 tcaactcgat tttatgatag gagctcgtcg ctccggtcca cgacacggtg atcaggccgc
      781 agacggttac gaaaaccccg gcgatcaccc atgggcccgg cctctcgcca aggaggagta
      841 tcgccagaac cgtggaaaac atcggcgttg tattgatgat cgtcgatgta accgagctgc
      901 ccatcgttcg aatgccgcgg aaactcagca cacgggttac gcccggcacc aacaaaccta
      961 cggcaacaaa aattagattg tcattaacca agattggcgc gaagggatca agtaagaaat
     1021 gcgccgataa tagaaacacg gtattgccgg caatggtaat ccaggcgccc cacggcgccg
     1081 cagcgaggtg atagccgcgc tgcgccaaga ggctggcgat ggcaaagcaa accgaagtca
     1141 aaatcccaaa gaagatcggc aacacggagc tttacaagtt tccttcgaaa acgatttcgt
     1201 ctagagtgcc cgttatagcc gaattttttt agaatcgcat tcatgctgcc aaccaatctt
     1261 gccctcctcc gccgcagact gctcggttgg tacgaaaaga acaggagaga tcttccctgg
     1321 cgcaggacat cggatcccta tgcgatctgg atttcggaaa ccatgctgca gcagacgcag
     1381 gtaaagacgg tcgttcctta ttatgaaagg tttctgaaag cgtttccgac catcgcagca
     1441 ttggatcacg cgccgcatcg acaggtgctg cgtgcctggt ccggtctagg atattatcgc
     1501 cgagcggaga acctaaagaa gtccgcctcg gagctgatgc ggcgacatgg cggaatgatg
     1561 ccgcaggatt acgagagttt gcgtgcgctg cccggaatcg gcgattacac cgccggcgct
     1621 attctcagca tcgccttcca gaaagcgtac ccggctgttg acggcaatgc gcggcgagtt
     1681 ttgggccgtt tgttcaactt ggccggggcg aagcaggtgc gcgccgcggc agaaaagctc
     1741 accccgaagt caaacccggg caatttcaac caggcggtta tggagctcgg cgcaaccatt
     1801 tgtcttcacg ccgagccgct ctgttcagtt tgtccgctgg tcaagcactg ccggctacac
     1861 agcggcggcc gaagctccaa gaagctggcc tctcggaaga aactcaaagt gagaaatgtt
     1921 acttggccaa tggcgattat tcgccgcggt gaaaaaattc tactacggcg ccgcggcaaa
     1981 accggcttgc tcgccggaat gtgggagctt cccggcggcg aggagtcatc caaccatggg
     2041 gttctgagaa ctttaagccg ccatatgcag ggaatcggcc ctgttttacc tatccctctg
     2101 agcatcggcg aaatccgtca tagcattaca aatcgtcgca ttcgctcccc cattttcctg
     2161 ttcgatctcg ccgtgaacga acccatcggt ttgcccggct ccggctggcg ctgggtaacc
     2221 ccggccgcgc tggaccgcta tccggcatcc tcgatgactc tcaaagcggc aagggttcta
     2281 gccagctatg aaaagagtgc ttcttaaaag cctgctcctt tcggcgataa tcgtatccct
     2341 ggttcatttt taccttgctt tgcccaacgt ttcgctcttg aagaccagaa atcctcgaag
     2401 ctcggcgctt atggaactac gcgaccagga gaacagagag aagaaattgc gggtgtcgcg
     2461 gcagcaaatt tgggtgcctt acggacggat ttcaaatcat ctaaagaaag ctgttctgct
     2521 cagcgaagac gcttctttct tcagtcataa cggagtcgat cttgacgagc tcaaggaatc
     2581 attgaaaaga gatttaacaa cgttcagctt caaacgcggc ggcagcacca tcacaatgca
     2641 gctagcgaga aacttatttt taaacccggc taagaatccc ctgcggaaaa tcaaggaaat
     2701 catcatcgcc tggcagctcg agcaggctct ttctaagatt cggattttcg agatttatct
     2761 aaacgtcgtc gagtggggcc gcaatattta cggcgctgaa gcagggtccc gtcactattt
     2821 tggcaaagcc gccgccactt tggatccttt ggaagccgcg actctagtgg cgttacttcc
     2881 caacccccga acaactcgag aaaaaagcat tcttcaaagg cgaaacctgg tgttagcaag
     2941 attggccgaa gtaggccata tcagtaagga ggaatacgat cgagtcaagc aaatcccgct
     3001 attccacaca gtaaagcaag aagctcccgg tctgctgcaa gattaaaatc cgggcggata
     3061 cccccaaata cccttgattc acagtacatc ctttctcttt ccatggcgtc taaactatcg
     3121 aggcgcaatc tccggcgaat gatagcctca acctcatcgc cagaacgtaa tcgacattcg
     3181 cctgaaaaaa gaaaggaaac gaatggaaaa agggacaaaa aagctggaca ctacccttgg
     3241 cgagctcatt gccgcggcca gcgaagttgc ttttgaatat tccgataacg acagagatgc
     3301 ctataagctg gctcaggcag cactgattga aattctcagg aaagcgtccc atgctggcag
     3361 cctagatgat tttggtgaac cggcgacaaa aaaaacttat cttcactaac cccaccatcg
     3421 aattgggggc ggatttccgc cccctaagtc cctcccggcg caggattcca tcgcagcctg
     3481 aagtgccaac aacgattgta aatggttcga ccaagttgca accaaacttg ctattcgatc
     3541 atctaaaact agagcactcg ttaaatccgc ggcttgaaga acatacggcc atgaatgagg
     3601 gggtgccgtg ctcagcagga tgagtacgtg gttgaattag gctaaatcta gttggacaat
     3661 tgacccatga tccgctacag acatatcgcc gggtcgccga ggactcctag tagaagtctt
     3721 agggacctgc gaaacagact aagcgaggac tcatgctgat tagaagacca gaggatatca
     3781 agtcctcaga gattaccccc aaggagctgt attttaatcg tcgacaattc atgcttgcag
     3841 cctcgggtgc tgccgccgcg gcaggcgcgg ctctcgctgg attcgacgtt ttcagacgtt
     3901 caggccacgc tcttgccgcc gaacagattg ccaatgtcaa gaagagctcc tacagcacta
     3961 gtgagaagca aaactcattt aaggacatca ccaactataa taacttctac gagcttgggc
     4021 cggacaaaga agatcccgcg gcaaacgcga agtatttcac tccaactcgt ccctggacga
     4081 tagccgtcga aggtgaggtc aaaaatccta agacgtacga catcgatgcc ttgacgaagc
     4141 taagtccgct cgaggagaga gtttatcgat tacggtgtgt cgaagcttgg tcgatggtga
     4201 tcccgtggat cggctttcct ttaagtgggc ttatcaaact cgccgaaccc accggcaacg
     4261 caaagtttat ccagttcgtc actttgcacg atcccaagcg catgcccggc caaaaattgc
     4321 cgattatgga gtggccgtac gtcgaaggct tgcgtatgga cgaagccatg cacccattga
     4381 ctatattatc ggtgggcctg tatggccagg tactgccggc gccgaacggc gcgccgattc
     4441 gggtggtggt gccgtggaag tacggtttca aaagcatcaa atccatcgtc aaaatccgat
     4501 tcgttgaaaa acaaccacca gcctcgtgga atatgatgca gccgcaggag tacggctttt
     4561 attccaatgt caatccagag gtcgatcatc cgcgttggac gcaggcaacg gagcgccgca
     4621 taggggagtt tctccggcgc aagacgctca tgttcaatgg ttacggcgat caggttgcgc
     4681 agctatacag cggcatggac ctgaagaaac tttattaagc atccgcggtt gggatataag
     4741 ttgctctcca tttttttcga ggtttgattc cgctcctacg gcaatgccgg tggtgcattt
     4801 ctaggcgcgg ttttgtctcc tttgtagtta accgatgcgc tcctggataa aacctgctct
     4861 atttaccgcc tgcttgatcc cgctagcaca gctcgcgtat ggattttata ccgacgatct
     4921 aaccgccaat ccgatcgaat acatcacccg cttcaccggc tcgtgggccc tgatcctttt
     4981 gattaccagc ctggcgatca ccccgctgcg caaaatattc ggctggaacc tgatcagata
     5041 tcgccgcatg ctgggcctgt ttgccttctt ctatgcgctc ctgcatttct caacctatat
     5101 tgtcctggac catttcttcg attttcaggc aattgccaag gacatttaca agagacctta
     5161 tgtcaccgct ggattcactg catttgtctt gatggttccg ctggcgctca cttcgacggc
     5221 ggcaatgatc cggcgcctgg gcaaacgttg gcaacagttg caccggctgg cctacgttgc
     5281 cgcggccgct ggagttcttc atttttactg gctggtaaaa tccgatattc gccgccctgt
     5341 acaatacggc gccgtgttag cattgctcct ggctgtgcgg cttgcgtatg cattactacc
     5401 catgccatca aagcgtatta ccggtaaagc taaaatcatc gcgccgcaaa ccgaccgctc
     5461 tcggttagca ggcaggcctt aaaggatcat tcgatcggac ggggctcgcc cctctccggc
     5521 ggatttacca cggtgccgat tattggcccc tccaggagat tgaccggcca aacccgaatt
     5581 ttagtaccgc ctcttgcgcg tcgcgccggc accgaagagt gagccgagga cgccgcgaat
     5641 tatctcccga ccgacctggc tgccgatggc gcgggccgcg cttttaccca tggcatcgaa
     5701 caaacctccc gcttccgatt gccacgagcc gctcgccgtc ttggcaggcg gctcttcggg
     5761 aagcggcgct cgagctttga gcatctcgta cgccgactca cggtcgacca ttttttcata
     5821 gtggccgaaa atcgtcgatc gtttcacgac agtttcgcgt tcggacgccg agagcggcgg
     5881 cagttggctc tgcggcgggt aaatcaaggc gcgatcaacg atgttaggcc ggcctttctc
     5941 gtccaggaag gaaaccagcg cttcaccgac agctagttca gtaatcgccg cttcaacgtc
     6001 gatggccgag ttctgacgaa atgtctgggc ggcggctttg acagcctttt gatcgcgcgg
     6061 cgtaaacgcg cgcaacgcgt gctggacacg atgacccaat tgaccgagca cgacatcggg
     6121 aacatccaat ggattttgcg tgacgaaata cacgccgatg cccttggagc gaatcagccg
     6181 gatcacctgc tcgatttttt cgagcaacac ggccggcgcg tcgttgaaaa gtaaatgcgc
     6241 ttcatcgaaa aaaaatatca gcttgggctt ctccggatcg cccacttccg gcagccgctc
     6301 gaacagctcc gacaacagcc aaagtaaaaa agtcgcatat agcttgggag cgttaatgag
     6361 ctgatcggca acaaggacat tgataatgcc ccgtccttga ctgtcggttt gcatcagatc
     6421 atcg
//
LOCUS       DASUVS010000491         4339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1821
            k141_217548, whole genome shotgun sequence.
ACCESSION   DASUVS010000491 DASUVS010000000
VERSION     DASUVS010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961154
            Sequence Read Archive: SRR25158387
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:39:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,088
            Genes (coding)                    :: 5,053
            CDSs (with protein)               :: 5,053
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 23 of 35
            Pseudo Genes (internal stop)      :: 4 of 35
            Pseudo Genes (multiple problems)  :: 4 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217548"
                     /isolate="SMAG_U1821"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="25.58666667 N 100.32 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VF182_25655"
     CDS             complement(<1..204)
                     /locus_tag="VF182_25655"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX6440530.1"
                     /translation="MRRFEILEPKSIAEACKILAAEEDVKLIAGGTALLILIKQGIFV
                     PKTLINLKKIKGAGEITYNSTDGL"
     gene            complement(491..1576)
                     /locus_tag="VF182_25660"
     CDS             complement(491..1576)
                     /locus_tag="VF182_25660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6440531.1"
                     /translation="MAEAYRLYAGTQAGMILFRGVADIWTSMGGSREFEPYFKDRVVD
                     SVYGCKNSPEIVFAGVTFDGLYRTKDAGRHWQRTLEGDIRWVTVDPTDDRVIYAGTEP
                     VHLYRSEDGGDTWEELKSLTGMPAEVQYKWYFPHPPHQGHVRHIYIDPEDSNCIYLAL
                     EHGGIVRSFDRGKTWEDVSEGLEYPDIHMIVRAPGNKTKYFTATSRGFFRADPPEKGW
                     KRAEKGMESDYFHDVINLPGDRPAMFLAAGHGSPQYWDRPGGVDGRIYRSEDGGETWR
                     QVGAGLPVKHTAMCWQLAYNPYDTNSIFAAFGDVARGHTAGPPGEGAIYVTYDRGDTW
                     EEVGLQKRGTKYLRPPAIRVLWAAADE"
     gene            complement(1704..2447)
                     /locus_tag="VF182_25665"
     CDS             complement(1704..2447)
                     /locus_tag="VF182_25665"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6440532.1"
                     /translation="MANKIAVVTGGAGGIGQAVVARLAQSGFGVCILDQNDKAGQEFV
                     EQLRKDKYEAEFYRVELRRAPEIKEVFETVYAKQRCIDVLVNLAGGTLHKHPIHEFPL
                     SEWREVIDVNLKATFLCCQAVSKRMKEAKSGAIINTSSNFGITGSAGRTAYSAAKAAI
                     IAFSKSLALEMAPYGVRVNTVAPGLTATARVLAVHTDAEWAKLSAGMPLGRAGEPRDI
                     AEGVHFLASEDSAFMTGQLLHVNGGMVFN"
     gene            complement(2481..3230)
                     /locus_tag="VF182_25670"
     CDS             complement(2481..3230)
                     /locus_tag="VF182_25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6440533.1"
                     /translation="MERLKNRVAIVTGAGQGIGRALALGFARAGAKVVIADINEESAA
                     SVKSEIEALGGTASAACTDVSNEDSVQTMVDKSLGEFGKLDILVNNAGIFPVSSVEEM
                     KEAEWDRVIGTNLVGAFLCSRAVAGKFLQQGSGRIISMTSGRAFQGAKNAAHYASSKA
                     GIIGFTKSLALELAPRQITVNVICPGITDTAQPRGHQSEEQIYAQAQKIPLGRIGQPE
                     DLVGTAVFLASDAAGFITGQTVIVNGGSIMW"
     gene            complement(3231..4019)
                     /locus_tag="VF182_25675"
     CDS             complement(3231..4019)
                     /locus_tag="VF182_25675"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX6440534.1"
                     /translation="MTKAIDLLEELSRARKPLALKDLARNLNLDKATAYRLLNSLRQK
                     GFVQRIDDGGSYGLGPKLLVLAEDYRRCFTMRDTVVPYLEQLVQVTGETAIYCERFQY
                     DSCVTIERRESPHHTRTVIETGVPRPLYVGSSACAILAVLPQEEILSILNVKKIEKFT
                     PFTITSPKQMLKKIEDIKRRGYAVSIQERYSYTAGVAAPCFLSRDLVGSIAVIGPAER
                     IRANGIEKTGKTVKKFAERLSSTFDYSKGPQSSGASGKRLRMVK"
BASE COUNT          954 a         1276 c         1081 g         1028 t
ORIGIN      
        1 caaaccgtct gtggagttgt aggtgatttc tccggcgcct ttaatctttt tcaaattgat
       61 taaagttttc ggaacgaaga tgccctgctt gatcaagatc agcagagcag tgccgccggc
      121 aatgagcttg acgtcctctt cggctgcaag aattttgcag gcttcggcaa tcgattttgg
      181 ttccaggatt tcaaatcgtc gcatgtgata cctagcccgc tgaccatggt gcagcgcgac
      241 cggcgaatgt gcatcggcta gtgccgtgtc tcacgctgtt agtagtttat ccggagcaca
      301 gcaggactcg gcccagcgca atcagccgca acccaacgaa gtattctccc gccaagacgc
      361 aaaggccgca aagcaagttg attctgaact cggcgatctt ggcgctttgc cgcgaggaaa
      421 ttcggtctca aagctcgaaa aataagtgcg ttcatttaag agccactaca ctaattgtgc
      481 ttccattaat ctattcgtcg gcagccgccc aaagaacccg gatcgccggc ggccgcaaat
      541 acttcgtgcc gcgcttttgc aagccgactt cttcccaggt atcgccgcgg tcataggtga
      601 catagatcgc gccttcgccc ggagggcctg ccgtatgacc gcgcgcgaca tcgccgaacg
      661 cggcgaaaat agaattggta tcgtacgggt tatacgcaag ctgccagcac atcgcggtat
      721 gcttcaccgg caatcctgcg cccacctgcc gccaggtctc accgccatct tcactgcgat
      781 agatgcggcc gtcgactccg cccggccgat cccaatactg cggtgagcca tgcccggcgg
      841 ccaaaaacat tgccggccgg tcgccgggca gattgatcac gtcatgaaag tagtcgctct
      901 ccatgccttt ttcggcgcgt ttccagcctt tctccggcgg atcggcacgg aaaaagccgc
      961 gcgatgtcgc ggtgaagtat tttgttttgt tgcccggtgc tctgacgatc atgtgtatgt
     1021 cgggatactc gagcccttca ctgacatcct cccaggtctt gccccggtca aagcttctaa
     1081 cgatgccgcc atgttcgaga gctaggtaaa tacagttcga atcctccgga tcgatgtaaa
     1141 tatgacgcac gtgcccctgg tgcggcggat gcggaaaata ccatttatac tgaacttcgg
     1201 ccggcatccc ggtaagcgat ttaagctcct cccaggtatc gccgccgtcc tcactgcgat
     1261 agagatgaac cggttcggtt ccggcgtaaa tgactcggtc atcagtcgga tcgacggtga
     1321 cccagcgaat atcgccctcg agggtgcgct gccaatgccg gccggcatct ttggtgcgat
     1381 acaaaccgtc gaaggttacg cccgcaaaga caatctccgg agaattttta catccataaa
     1441 cggaatccac cactcgatct ttgaagtacg gctcaaattc cctcgaaccg cccatcgaag
     1501 tccaaatatc agcgaccccc cgaaataaaa tcatccccgc ctgagtaccc gcataaagtc
     1561 ggtaggcttc agccattttc gtcccttccg aaatttagtt tgtttccaga ctagcgagag
     1621 gcgcaaagag caaggagcta ggaccaaaca gagggcgcca gccctttgcc cgcgcgctta
     1681 gcccttcgcg atcagccacg atcttagttg aaaaccattc cgccatttac atgcagcaac
     1741 tgacccgtca taaaggcgct atcctcactg gcgagaaaat gaacaccttc agcgatatcc
     1801 ctcggttctc cggctcggcc aagcggcatg ccggcgctca acttcgccca ttcggcatcg
     1861 gtatgaaccg ccaacacccg cgctgtggcg gtgagacccg gggcaacggt attgacgcgc
     1921 acaccgtaag gcgccatttc cagggccaat gatttagaaa aagcaatgat ggccgctttg
     1981 gcggccgagt aagcggtcct cccggcgcta ccggtgatgc cgaaattgga ggacgtgttg
     2041 ataatggcgc cgcttttggc ttccttcata cgcttgctca cggcctgaca acagagaaac
     2101 gtcgctttca gattgacatc gatcacttcg cgccactccg agagcggaaa ctcatgaatc
     2161 ggatgcttgt gcagcgttcc gccggctaga ttcaccagca cgtcgatgca ccgctgcttt
     2221 gcatagaccg tttcgaatac ttctttgatt tcaggggcgc gtctcagctc gactcgatag
     2281 aattcggcct catacttgtc tttgcgcaac tgctcgacaa actcctgccc ggctttgtcg
     2341 ttttgatcga ggatgcagac gccgaagcca ctttgcgcca gccgagccac tacagcctgg
     2401 ccgattccac ccgcgccccc agtcactact gcaatcttat ttgccatggt ttcactctgc
     2461 tgtcgaaacg gacctttcga ttaccacatg atgctgccgc cgttgacgat caccgtctgc
     2521 ccggtgatga agccggcggc atcgcttgcc aagaacaccg cagtgcctac caggtcttcg
     2581 ggctggccga ttcggcccag cggaatcttt tgggcttgag cataaatttg ctcctcgctc
     2641 tgatggccgc gcggttgcgc cgtatccgtg attccggggc aaatcacgtt caccgtgatc
     2701 tgacgcggcg ccagctctaa cgccaatgat tttgtaaaac cgatgatgcc ggcctttgac
     2761 gatgcgtagt gagcggcatt tttggcgccc tggaatgccc gtcccgaagt catgctgatg
     2821 attctgccac ttccctgctg caaaaatttg cccgccacgg ctctcgaaca gagaaatgcc
     2881 cccaccaggt ttgtaccgat gacacgatcc cactccgctt ctttcatttc ttcgacggag
     2941 ctgaccggga aaatgcctgc gttgttgact aaaatatcaa gcttgccgaa ctctcctaac
     3001 gacttatcga ccatcgtttg gacggagtcc tcattcgaga catcggtaca cgctgccgag
     3061 gctgttccgc ctaaggcttc gatctcgctt ttgaccgatg cggcgctttc ctcattgata
     3121 tcggcaatga cgaccttcgc gcccgcgcga gcgaacccga gcgccagcgc tctgccgatt
     3181 ccctgccccg cccccgtaac tatagcaacc cgatttttta gtcgctccat ttacttgacc
     3241 atccgcaatc gtttcccact cgcacctgag ctttgtgggc ctttagaata gtcaaacgtg
     3301 ctggatagac gttcggcaaa cttcttgact gtcttccccg ttttctcaat tccatttgcc
     3361 cgaattcgct ctgcaggtcc tataacggct atgctgccga ctaggtctct gctaaggaaa
     3421 catggagcag caacaccggc ggtgtacgag tatctttctt gaatgctcac cgcatagccg
     3481 cgtcgtttga tgtcctcgat ctttttcagc atctgcttcg gcgaggttat cgtgaagggt
     3541 gtgaatttct caattttttt cacgttgagt atcgatagaa tctcttcctg cggcagcact
     3601 gccagaatcg cgcatgcgga cgagcccaca tagagagggc gcggaactcc ggtttcgatg
     3661 accgtgcgcg tgtgatgcgg actttcacgc cgttctatcg tcacacacga gtcgtactga
     3721 aatcgttcac aataaatcgc cgtctctccc gtcacctgaa ccagttgttc caggtaggga
     3781 acgactgtat cccgcatggt aaagcatctg cgataatcct cggcaagcac taacagcttc
     3841 ggtccaagac cataagaacc gccgtcgtcg attctttgga caaagccctt ttgtctcaag
     3901 gaattcagaa ggcggtaggc cgttgcttta tccaaattga gattccgcgc aagatccttc
     3961 aacgcgagcg gcttcctcgc ccggctcaac tcctctaaaa ggtcgatcgc tttggtcact
     4021 gaagcattca ttttaataat tgaaatgacg ttttggcggc atcaccttcg aaatatatta
     4081 ccgacaaaca gctgtcaaca acatcgaaag atgttttaga acatggagca agagcaaccg
     4141 gtagaaaacc aggttcatca ctggcgcgat ctgcaactag cttcgggcga ttgtgcggac
     4201 taccgtctga ggttcgggag cgattccgta cgcgtgctca gattcgattt gacggacgtg
     4261 gactctatca gcaaatcctt cacgtaaatc gctgagcgac tcgtggtact ccgcgtgaag
     4321 tgaaaactaa atggaatgc
//
